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PMC:2664230 / 8811-10800 JSONTXT 17 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4475 0-8 NN denotes SDS-PAGE
T4476 9-12 CC denotes and
T4477 13-27 NN denotes Immunoblotting
T4478 28-29 -LRB- denotes (
T4479 29-34 NN denotes I-κBα
T4480 34-35 -COMMA- denotes ,
T4481 36-41 NN denotes NF-κB
T4482 42-45 NN denotes p65
T4483 45-46 -COMMA- denotes ,
T4484 47-50 CC denotes and
T4485 51-55 NN denotes CREB
T4486 55-56 -RRB- denotes )
T4487 57-66 VB denotes Following
T4488 67-78 NN denotes stimulation
T4489 78-79 -COMMA- denotes ,
T4490 80-85 NN denotes PBMCs
T4491 86-90 VB denotes were
T4492 91-97 VB denotes washed
T4493 98-102 IN denotes with
T4494 103-111 JJ denotes ice-cold
T4495 112-115 NN denotes PBS
T4496 116-119 CC denotes and
T4497 120-131 VB denotes centrifuged
T4498 132-134 TO denotes to
T4499 135-142 VB denotes collect
T4500 143-144 DT denotes a
T4501 145-149 NN denotes cell
T4502 150-156 NN denotes pellet
T4503 158-161 DT denotes The
T4504 162-168 NN denotes pellet
T4505 169-172 VB denotes was
T4506 173-184 VB denotes resuspended
T4507 185-187 IN denotes in
T4508 188-196 JJ denotes ice-cold
T4509 197-200 NN denotes SDS
T4510 201-207 NN denotes sample
T4511 208-214 NN denotes buffer
T4512 215-227 VB denotes supplemented
T4513 228-232 IN denotes with
T4514 233-236 CD denotes 100
T4515 237-239 NN denotes mM
T4516 240-254 NN denotes dithiothreitol
T4517 256-267 JJ denotes Cytoplasmic
T4518 268-271 CC denotes and
T4519 272-279 JJ denotes nuclear
T4520 280-288 NN denotes extracts
T4521 289-293 VB denotes were
T4522 294-302 VB denotes isolated
T4523 303-307 IN denotes with
T4524 308-314 NN denotes NE-PER
T4525 315-322 JJ denotes nuclear
T4526 323-326 CC denotes and
T4527 327-338 JJ denotes cytoplasmic
T4528 339-349 NN denotes extraction
T4529 350-358 NN denotes reagents
T4530 359-363 IN denotes with
T4531 364-366 NN denotes 1x
T4532 367-371 NNP denotes Halt
T4533 372-380 NNP denotes Protease
T4534 381-390 NNP denotes Inhibitor
T4535 391-399 NNP denotes Cocktail
T4536 400-409 VB denotes according
T4537 410-412 TO denotes to
T4538 413-416 DT denotes the
T4539 417-431 NN denotes manufacturer’s
T4540 432-444 NN denotes instructions
T4541 446-453 NN denotes Protein
T4542 454-468 NN denotes concentrations
T4543 469-473 VB denotes were
T4544 474-484 VB denotes determined
T4545 485-489 IN denotes with
T4546 490-493 DT denotes the
T4547 494-497 NN denotes BCA
T4548 498-505 NN denotes protein
T4549 506-513 NN denotes reagent
T4550 514-517 NN denotes kit
T4551 518-521 IN denotes for
T4552 522-526 DT denotes each
T4553 527-533 NN denotes sample
T4554 534-543 VB denotes according
T4555 544-546 TO denotes to
T4556 547-548 DT denotes a
T4557 549-561 JJ denotes standardized
T4558 562-567 NN denotes curve
T4559 568-571 IN denotes for
T4560 572-579 NN denotes albumin
T4561 581-585 NN denotes Cell
T4562 586-593 NN denotes lysates
T4563 594-598 VB denotes were
T4564 599-608 VB denotes collected
T4565 609-611 IN denotes by
T4566 612-619 VB denotes boiling
T4567 620-623 DT denotes the
T4568 624-631 NN denotes samples
T4569 632-634 IN denotes in
T4570 635-636 DT denotes a
T4571 637-642 NN denotes 100ºC
T4572 643-648 NN denotes water
T4573 649-653 NN denotes bath
T4574 654-657 IN denotes for
T4575 658-659 CD denotes 5
T4576 660-667 NN denotes minutes
T4577 669-672 CD denotes Ten
T4578 673-675 NN denotes μg
T4579 676-678 IN denotes of
T4580 679-686 NN denotes protein
T4581 687-690 IN denotes per
T4582 691-697 NN denotes sample
T4583 698-701 VB denotes was
T4584 702-711 VB denotes separated
T4585 712-714 IN denotes by
T4586 715-733 NN denotes SDS-polyacrylamide
T4587 734-737 NN denotes gel
T4588 738-753 NN denotes electrophoresis
T4589 754-761 IN denotes through
T4590 762-766 CD denotes 8–16
T4591 766-767 NN denotes %
T4592 768-780 NN denotes tris-glycine
T4593 781-795 NN denotes polyacrylamide
T4594 796-804 NN denotes gradient
T4595 805-809 NN denotes gels
T4596 810-813 CC denotes and
T4597 814-825 VB denotes transferred
T4598 826-828 TO denotes to
T4599 829-843 VB denotes nitrocellulose
T4600 844-853 NN denotes membranes
T4601 855-858 DT denotes The
T4602 859-868 NN denotes membranes
T4603 869-873 VB denotes were
T4604 874-881 VB denotes blocked
T4605 882-886 IN denotes with
T4606 887-888 CD denotes 5
T4607 888-889 NN denotes %
T4608 890-894 NN denotes milk
T4609 895-897 IN denotes in
T4610 898-911 JJ denotes Tris-buffered
T4611 912-924 NN denotes saline/Tween
T4612 925-927 CD denotes 20
T4613 928-929 -LRB- denotes (
T4614 929-936 NNP denotes Fischer
T4615 937-947 NNP denotes Scientific
T4616 947-948 -COMMA- denotes ,
T4617 949-959 NNP denotes Pittsburgh
T4618 959-960 -COMMA- denotes ,
T4619 961-963 NNP denotes PA
T4620 963-964 -RRB- denotes )
T4621 965-968 IN denotes for
T4622 969-970 CD denotes 1
T4623 971-975 NN denotes hour
T4624 977-988 JJ denotes Cytoplasmic
T4625 989-997 NN denotes extracts
T4626 998-1002 VB denotes were
T4627 1003-1012 VB denotes incubated
T4628 1013-1017 IN denotes with
T4629 1018-1032 VB denotes phosphorylated
T4630 1033-1038 JJ denotes I-κBα
T4631 1039-1047 NN denotes antibody
T4632 1048-1049 -LRB- denotes (
T4633 1049-1050 CD denotes 1
T4634 1050-1051 -COLON- denotes :
T4635 1051-1054 CD denotes 200
T4636 1054-1055 -RRB- denotes )
T4637 1055-1056 -COMMA- denotes ,
T4638 1057-1062 IN denotes while
T4639 1063-1070 JJ denotes nuclear
T4640 1071-1079 NN denotes extracts
T4641 1080-1084 VB denotes were
T4642 1085-1094 VB denotes incubated
T4643 1095-1099 IN denotes with
T4644 1100-1106 CC denotes either
T4645 1107-1121 VB denotes phosphorylated
T4646 1122-1127 NN denotes NF-κB
T4647 1128-1131 NN denotes p65
T4648 1132-1140 NN denotes antibody
T4649 1141-1142 -LRB- denotes (
T4650 1142-1143 CD denotes 1
T4651 1143-1144 -COLON- denotes :
T4652 1144-1147 CD denotes 500
T4653 1147-1148 -RRB- denotes )
T4654 1149-1151 CC denotes or
T4655 1152-1166 VB denotes phosphorylated
T4656 1167-1171 NN denotes CREB
T4657 1172-1180 NN denotes antibody
T4658 1181-1182 -LRB- denotes (
T4659 1182-1183 CD denotes 1
T4660 1183-1184 -COLON- denotes :
T4661 1184-1187 CD denotes 500
T4662 1187-1188 -RRB- denotes )
T4663 1189-1198 RB denotes overnight
T4664 1199-1201 IN denotes at
T4665 1202-1205 NN denotes 4°C
T4666 1206-1208 IN denotes in
T4667 1209-1217 JJ denotes separate
T4668 1218-1229 NN denotes experiments
T4669 1231-1234 DT denotes The
T4670 1235-1244 NN denotes membranes
T4671 1245-1249 VB denotes were
T4672 1250-1256 VB denotes washed
T4673 1257-1261 IN denotes with
T4674 1262-1275 JJ denotes Tris-buffered
T4675 1276-1288 NN denotes saline/Tween
T4676 1289-1291 CD denotes 20
T4677 1292-1295 CC denotes and
T4678 1296-1305 VB denotes incubated
T4679 1306-1309 IN denotes for
T4680 1310-1311 CD denotes 1
T4681 1312-1316 NN denotes hour
T4682 1317-1319 IN denotes at
T4683 1320-1324 NN denotes room
T4684 1325-1336 NN denotes temperature
T4685 1337-1341 IN denotes with
T4686 1342-1345 DT denotes the
T4687 1346-1355 JJ denotes secondary
T4688 1356-1364 NN denotes antibody
T4689 1364-1365 -COMMA- denotes ,
T4690 1366-1377 JJ denotes horseradish
T4691 1378-1395 JJ denotes peroxidase-linked
T4692 1396-1407 JJ denotes anti-rabbit
T4693 1408-1411 NN denotes IgG
T4694 1412-1419 VB denotes diluted
T4695 1420-1421 CD denotes 1
T4696 1421-1422 -COLON- denotes :
T4697 1422-1426 CD denotes 2000
T4698 1427-1429 IN denotes in
T4699 1430-1438 VB denotes blocking
T4700 1439-1447 NN denotes solution
T4701 1449-1454 IN denotes After
T4702 1455-1463 VB denotes repeated
T4703 1464-1471 NN denotes washing
T4704 1472-1474 IN denotes of
T4705 1475-1478 DT denotes the
T4706 1479-1487 NN denotes membrane
T4707 1487-1488 -COMMA- denotes ,
T4708 1489-1492 DT denotes the
T4709 1493-1504 NNP denotes Supersignal
T4710 1505-1509 NNP denotes West
T4711 1510-1514 NNP denotes Pico
T4712 1515-1531 NNP denotes Chemiluminescent
T4713 1532-1535 NNP denotes Kit
T4714 1536-1539 VB denotes was
T4715 1540-1547 VB denotes applied
T4716 1548-1551 IN denotes for
T4717 1552-1560 NN denotes antibody
T4718 1561-1570 NN denotes detection
T4719 1571-1574 IN denotes per
T4720 1575-1578 DT denotes the
T4721 1579-1593 NN denotes manufacturer’s
T4722 1594-1606 NN denotes instructions
T4723 1608-1615 JJ denotes Western
T4724 1616-1620 NN denotes blot
T4725 1621-1625 NN denotes data
T4726 1626-1628 VB denotes is
T4727 1629-1638 VB denotes presented
T4728 1639-1641 IN denotes as
T4729 1642-1643 DT denotes a
T4730 1644-1654 NN denotes percentage
T4731 1655-1657 IN denotes of
T4732 1658-1661 NN denotes LPS
T4733 1662-1673 NN denotes stimulation
T4734 1675-1678 DT denotes The
T4735 1679-1689 NN denotes percentage
T4736 1690-1692 IN denotes of
T4737 1693-1696 NN denotes LPS
T4738 1697-1708 NN denotes stimulation
T4739 1709-1712 VB denotes was
T4740 1713-1723 VB denotes calculated
T4741 1724-1726 IN denotes by
T4742 1727-1735 VB denotes dividing
T4743 1736-1739 DT denotes the
T4744 1740-1744 JJ denotes mean
T4745 1745-1749 NN denotes band
T4746 1750-1755 NN denotes pixel
T4747 1756-1761 NN denotes total
T4748 1762-1765 IN denotes for
T4749 1766-1770 DT denotes each
T4750 1771-1780 NN denotes treatment
T4751 1781-1784 NN denotes arm
T4752 1785-1792 VB denotes divided
T4753 1793-1795 IN denotes by
T4754 1796-1799 DT denotes the
T4755 1800-1804 JJ denotes mean
T4756 1805-1810 NN denotes pixel
T4757 1811-1816 NN denotes total
T4758 1817-1819 IN denotes of
T4759 1820-1827 NN denotes samples
T4760 1828-1838 VB denotes stimulated
T4761 1839-1843 IN denotes with
T4762 1844-1847 NN denotes LPS
T4763 1848-1851 CC denotes and
T4764 1852-1863 VB denotes multiplying
T4765 1864-1866 IN denotes by
T4766 1867-1870 CD denotes 100
T4767 1872-1876 RB denotes Thus
T4768 1876-1877 -COMMA- denotes ,
T4769 1878-1881 NN denotes LPS
T4770 1882-1893 NN denotes stimulation
T4771 1894-1896 VB denotes is
T4772 1897-1905 VB denotes reported
T4773 1906-1908 IN denotes as
T4774 1909-1912 CD denotes 100
T4775 1912-1913 NN denotes %
T4776 1914-1917 CC denotes and
T4777 1918-1922 DT denotes each
T4778 1923-1925 IN denotes of
T4779 1926-1929 DT denotes the
T4780 1930-1939 NN denotes treatment
T4781 1940-1944 NN denotes arms
T4782 1945-1947 VB denotes is
T4783 1948-1956 VB denotes reported
T4784 1957-1959 IN denotes as
T4785 1960-1961 DT denotes a
T4786 1962-1969 NN denotes percent
T4787 1970-1972 IN denotes of
T4788 1973-1976 NN denotes LPS
T4789 1977-1988 NN denotes stimulation
R3696 T4475 T4476 arg1Of SDS-PAGE,and
R3697 T4476 T4478 arg1Of and,(
R3698 T4477 T4476 arg2Of Immunoblotting,and
R3699 T4479 T4480 arg1Of I-κBα,","
R3700 T4480 T4484 arg1Of ",",and
R3701 T4482 T4480 arg2Of p65,","
R3702 T4482 T4481 arg1Of p65,NF-κB
R3703 T4484 T4478 arg2Of and,(
R3704 T4484 T4483 arg1Of and,","
R3705 T4485 T4484 arg2Of CREB,and
R3706 T4486 T4478 arg3Of ),(
R3707 T4488 T4487 arg2Of stimulation,Following
R3708 T4490 T4491 arg1Of PBMCs,were
R3709 T4490 T4492 arg2Of PBMCs,washed
R3710 T4490 T4497 arg2Of PBMCs,centrifuged
R3711 T4490 T4499 arg1Of PBMCs,collect
R3712 T4492 T4493 arg1Of washed,with
R3713 T4492 T4496 arg1Of washed,and
R3714 T4495 T4493 arg2Of PBS,with
R3715 T4495 T4494 arg1Of PBS,ice-cold
R3716 T4496 T4487 arg1Of and,Following
R3717 T4496 T4489 arg1Of and,","
R3718 T4496 T4491 arg2Of and,were
R3719 T4497 T4496 arg2Of centrifuged,and
R3720 T4499 T4497 arg3Of collect,centrifuged
R3721 T4499 T4498 arg1Of collect,to
R3722 T4502 T4499 arg2Of pellet,collect
R3723 T4502 T4500 arg1Of pellet,a
R3724 T4502 T4501 arg1Of pellet,cell
R3725 T4504 T4503 arg1Of pellet,The
R3726 T4504 T4505 arg1Of pellet,was
R3727 T4504 T4506 arg2Of pellet,resuspended
R3728 T4506 T4505 arg2Of resuspended,was
R3729 T4506 T4507 arg1Of resuspended,in
R3730 T4511 T4507 arg2Of buffer,in
R3731 T4511 T4508 arg1Of buffer,ice-cold
R3732 T4511 T4509 arg1Of buffer,SDS
R3733 T4511 T4510 arg1Of buffer,sample
R3734 T4511 T4512 arg2Of buffer,supplemented
R3735 T4512 T4513 arg1Of supplemented,with
R3736 T4514 T4515 arg1Of 100,mM
R3737 T4516 T4513 arg2Of dithiothreitol,with
R3738 T4516 T4514 arg1Of dithiothreitol,100
R3739 T4517 T4518 arg1Of Cytoplasmic,and
R3740 T4519 T4518 arg2Of nuclear,and
R3741 T4520 T4517 arg1Of extracts,Cytoplasmic
R3742 T4520 T4519 arg1Of extracts,nuclear
R3743 T4520 T4521 arg1Of extracts,were
R3744 T4520 T4522 arg2Of extracts,isolated
R3745 T4522 T4521 arg2Of isolated,were
R3746 T4522 T4523 arg1Of isolated,with
R3747 T4522 T4536 arg1Of isolated,according
R3748 T4525 T4526 arg1Of nuclear,and
R3749 T4527 T4526 arg2Of cytoplasmic,and
R3750 T4529 T4523 arg2Of reagents,with
R3751 T4529 T4524 arg1Of reagents,NE-PER
R3752 T4529 T4525 arg1Of reagents,nuclear
R3753 T4529 T4527 arg1Of reagents,cytoplasmic
R3754 T4529 T4528 arg1Of reagents,extraction
R3755 T4529 T4530 arg1Of reagents,with
R3756 T4535 T4530 arg2Of Cocktail,with
R3757 T4535 T4531 arg1Of Cocktail,1x
R3758 T4535 T4532 arg1Of Cocktail,Halt
R3759 T4535 T4533 arg1Of Cocktail,Protease
R3760 T4535 T4534 arg1Of Cocktail,Inhibitor
R3761 T4537 T4536 arg2Of to,according
R3762 T4540 T4537 arg2Of instructions,to
R3763 T4540 T4538 arg1Of instructions,the
R3764 T4540 T4539 arg1Of instructions,manufacturer’s
R3765 T4542 T4541 arg1Of concentrations,Protein
R3766 T4542 T4543 arg1Of concentrations,were
R3767 T4542 T4544 arg2Of concentrations,determined
R3768 T4544 T4543 arg2Of determined,were
R3769 T4544 T4545 arg1Of determined,with
R3770 T4544 T4554 arg1Of determined,according
R3771 T4550 T4545 arg2Of kit,with
R3772 T4550 T4546 arg1Of kit,the
R3773 T4550 T4547 arg1Of kit,BCA
R3774 T4550 T4548 arg1Of kit,protein
R3775 T4550 T4549 arg1Of kit,reagent
R3776 T4550 T4551 arg1Of kit,for
R3777 T4553 T4551 arg2Of sample,for
R3778 T4553 T4552 arg1Of sample,each
R3779 T4555 T4554 arg2Of to,according
R3780 T4558 T4555 arg2Of curve,to
R3781 T4558 T4556 arg1Of curve,a
R3782 T4558 T4557 arg1Of curve,standardized
R3783 T4558 T4559 arg1Of curve,for
R3784 T4560 T4559 arg2Of albumin,for
R3785 T4562 T4561 arg1Of lysates,Cell
R3786 T4562 T4563 arg1Of lysates,were
R3787 T4562 T4564 arg2Of lysates,collected
R3788 T4564 T4563 arg2Of collected,were
R3789 T4564 T4565 arg1Of collected,by
R3790 T4566 T4565 arg2Of boiling,by
R3791 T4566 T4569 arg1Of boiling,in
R3792 T4568 T4566 arg2Of samples,boiling
R3793 T4568 T4567 arg1Of samples,the
R3794 T4573 T4569 arg2Of bath,in
R3795 T4573 T4570 arg1Of bath,a
R3796 T4573 T4571 arg1Of bath,100ºC
R3797 T4573 T4572 arg1Of bath,water
R3798 T4573 T4574 arg1Of bath,for
R3799 T4576 T4574 arg2Of minutes,for
R3800 T4576 T4575 arg1Of minutes,5
R3801 T4578 T4577 arg1Of μg,Ten
R3802 T4578 T4579 arg1Of μg,of
R3803 T4578 T4581 arg1Of μg,per
R3804 T4578 T4583 arg1Of μg,was
R3805 T4578 T4584 arg2Of μg,separated
R3806 T4578 T4597 arg2Of μg,transferred
R3807 T4580 T4579 arg2Of protein,of
R3808 T4582 T4581 arg2Of sample,per
R3809 T4584 T4589 arg1Of separated,through
R3810 T4584 T4596 arg1Of separated,and
R3811 T4588 T4584 arg1Of electrophoresis,separated
R3812 T4588 T4585 arg2Of electrophoresis,by
R3813 T4588 T4586 arg1Of electrophoresis,SDS-polyacrylamide
R3814 T4588 T4587 arg1Of electrophoresis,gel
R3815 T4590 T4591 arg1Of 8–16,%
R3816 T4595 T4589 arg2Of gels,through
R3817 T4595 T4590 arg1Of gels,8–16
R3818 T4595 T4592 arg1Of gels,tris-glycine
R3819 T4595 T4593 arg1Of gels,polyacrylamide
R3820 T4595 T4594 arg1Of gels,gradient
R3821 T4596 T4583 arg2Of and,was
R3822 T4597 T4596 arg2Of transferred,and
R3823 T4597 T4598 modOf transferred,to
R3824 T4599 T4598 arg1Of nitrocellulose,to
R3825 T4600 T4599 arg2Of membranes,nitrocellulose
R3826 T4602 T4601 arg1Of membranes,The
R3827 T4602 T4603 arg1Of membranes,were
R3828 T4602 T4604 arg2Of membranes,blocked
R3829 T4604 T4603 arg2Of blocked,were
R3830 T4604 T4605 arg1Of blocked,with
R3831 T4606 T4607 arg1Of 5,%
R3832 T4608 T4605 arg2Of milk,with
R3833 T4608 T4606 arg1Of milk,5
R3834 T4608 T4609 arg1Of milk,in
R3835 T4611 T4609 arg2Of saline/Tween,in
R3836 T4611 T4610 arg1Of saline/Tween,Tris-buffered
R3837 T4611 T4612 arg1Of saline/Tween,20
R3838 T4611 T4613 arg1Of saline/Tween,(
R3839 T4611 T4621 arg1Of saline/Tween,for
R3840 T4615 T4613 arg2Of Scientific,(
R3841 T4615 T4614 arg1Of Scientific,Fischer
R3842 T4615 T4616 arg1Of Scientific,","
R3843 T4617 T4616 arg2Of Pittsburgh,","
R3844 T4617 T4618 arg1Of Pittsburgh,","
R3845 T4619 T4618 arg2Of PA,","
R3846 T4620 T4613 arg3Of ),(
R3847 T4623 T4621 arg2Of hour,for
R3848 T4623 T4622 arg1Of hour,1
R3849 T4625 T4624 arg1Of extracts,Cytoplasmic
R3850 T4625 T4626 arg1Of extracts,were
R3851 T4625 T4627 arg2Of extracts,incubated
R3852 T4627 T4626 arg2Of incubated,were
R3853 T4627 T4628 arg1Of incubated,with
R3854 T4627 T4637 arg1Of incubated,","
R3855 T4627 T4638 arg1Of incubated,while
R3856 T4631 T4628 arg2Of antibody,with
R3857 T4631 T4629 arg2Of antibody,phosphorylated
R3858 T4631 T4630 arg1Of antibody,I-κBα
R3859 T4631 T4632 arg1Of antibody,(
R3860 T4633 T4634 arg1Of 1,:
R3861 T4634 T4632 arg2Of :,(
R3862 T4635 T4634 arg2Of 200,:
R3863 T4636 T4632 arg3Of ),(
R3864 T4640 T4639 arg1Of extracts,nuclear
R3865 T4640 T4641 arg1Of extracts,were
R3866 T4640 T4642 arg2Of extracts,incubated
R3867 T4642 T4638 arg2Of incubated,while
R3868 T4642 T4641 arg2Of incubated,were
R3869 T4642 T4643 arg1Of incubated,with
R3870 T4642 T4664 arg1Of incubated,at
R3871 T4648 T4644 arg1Of antibody,either
R3872 T4648 T4645 arg2Of antibody,phosphorylated
R3873 T4648 T4646 arg1Of antibody,NF-κB
R3874 T4648 T4647 arg1Of antibody,p65
R3875 T4648 T4649 arg1Of antibody,(
R3876 T4648 T4654 arg1Of antibody,or
R3877 T4650 T4651 arg1Of 1,:
R3878 T4651 T4649 arg2Of :,(
R3879 T4652 T4651 arg2Of 500,:
R3880 T4653 T4649 arg3Of ),(
R3881 T4654 T4643 arg2Of or,with
R3882 T4657 T4654 arg2Of antibody,or
R3883 T4657 T4655 arg2Of antibody,phosphorylated
R3884 T4657 T4656 arg1Of antibody,CREB
R3885 T4657 T4658 arg1Of antibody,(
R3886 T4657 T4663 arg1Of antibody,overnight
R3887 T4659 T4660 arg1Of 1,:
R3888 T4660 T4658 arg2Of :,(
R3889 T4661 T4660 arg2Of 500,:
R3890 T4662 T4658 arg3Of ),(
R3891 T4665 T4664 arg2Of 4°C,at
R3892 T4665 T4666 arg1Of 4°C,in
R3893 T4668 T4666 arg2Of experiments,in
R3894 T4668 T4667 arg1Of experiments,separate
R3895 T4670 T4669 arg1Of membranes,The
R3896 T4670 T4671 arg1Of membranes,were
R3897 T4670 T4672 arg2Of membranes,washed
R3898 T4670 T4678 arg2Of membranes,incubated
R3899 T4672 T4673 arg1Of washed,with
R3900 T4672 T4677 arg1Of washed,and
R3901 T4675 T4673 arg2Of saline/Tween,with
R3902 T4675 T4674 arg1Of saline/Tween,Tris-buffered
R3903 T4675 T4676 arg1Of saline/Tween,20
R3904 T4677 T4671 arg2Of and,were
R3905 T4678 T4677 arg2Of incubated,and
R3906 T4678 T4679 arg1Of incubated,for
R3907 T4678 T4682 arg1Of incubated,at
R3908 T4681 T4679 arg2Of hour,for
R3909 T4681 T4680 arg1Of hour,1
R3910 T4684 T4682 arg2Of temperature,at
R3911 T4684 T4683 arg1Of temperature,room
R3912 T4684 T4685 arg1Of temperature,with
R3913 T4688 T4685 arg2Of antibody,with
R3914 T4688 T4686 arg1Of antibody,the
R3915 T4688 T4687 arg1Of antibody,secondary
R3916 T4693 T4690 arg1Of IgG,horseradish
R3917 T4693 T4691 arg1Of IgG,peroxidase-linked
R3918 T4693 T4692 arg1Of IgG,anti-rabbit
R3919 T4693 T4694 arg1Of IgG,diluted
R3920 T4694 T4671 modOf diluted,were
R3921 T4694 T4689 arg1Of diluted,","
R3922 T4694 T4696 arg1Of diluted,:
R3923 T4695 T4694 arg2Of 1,diluted
R3924 T4697 T4694 arg3Of 2000,diluted
R3925 T4697 T4698 arg1Of 2000,in
R3926 T4699 T4698 arg2Of blocking,in
R3927 T4700 T4699 arg2Of solution,blocking
R3928 T4703 T4701 arg2Of washing,After
R3929 T4703 T4702 arg2Of washing,repeated
R3930 T4703 T4704 arg1Of washing,of
R3931 T4706 T4704 arg2Of membrane,of
R3932 T4706 T4705 arg1Of membrane,the
R3933 T4713 T4708 arg1Of Kit,the
R3934 T4713 T4709 arg1Of Kit,Supersignal
R3935 T4713 T4710 arg1Of Kit,West
R3936 T4713 T4711 arg1Of Kit,Pico
R3937 T4713 T4712 arg1Of Kit,Chemiluminescent
R3938 T4713 T4714 arg1Of Kit,was
R3939 T4713 T4715 arg2Of Kit,applied
R3940 T4715 T4701 arg1Of applied,After
R3941 T4715 T4707 arg1Of applied,","
R3942 T4715 T4714 arg2Of applied,was
R3943 T4715 T4716 arg1Of applied,for
R3944 T4718 T4716 arg2Of detection,for
R3945 T4718 T4717 arg1Of detection,antibody
R3946 T4718 T4719 arg1Of detection,per
R3947 T4722 T4719 arg2Of instructions,per
R3948 T4722 T4720 arg1Of instructions,the
R3949 T4722 T4721 arg1Of instructions,manufacturer’s
R3950 T4725 T4723 arg1Of data,Western
R3951 T4725 T4724 arg1Of data,blot
R3952 T4725 T4726 arg1Of data,is
R3953 T4725 T4727 arg2Of data,presented
R3954 T4727 T4726 arg2Of presented,is
R3955 T4727 T4728 arg1Of presented,as
R3956 T4730 T4728 arg2Of percentage,as
R3957 T4730 T4729 arg1Of percentage,a
R3958 T4730 T4731 arg1Of percentage,of
R3959 T4733 T4731 arg2Of stimulation,of
R3960 T4733 T4732 arg1Of stimulation,LPS
R3961 T4735 T4734 arg1Of percentage,The
R3962 T4735 T4736 arg1Of percentage,of
R3963 T4735 T4739 arg1Of percentage,was
R3964 T4735 T4740 arg2Of percentage,calculated
R3965 T4738 T4736 arg2Of stimulation,of
R3966 T4738 T4737 arg1Of stimulation,LPS
R3967 T4740 T4739 arg2Of calculated,was
R3968 T4740 T4741 arg1Of calculated,by
R3969 T4742 T4741 arg2Of dividing,by
R3970 T4747 T4742 arg2Of total,dividing
R3971 T4747 T4743 arg1Of total,the
R3972 T4747 T4744 arg1Of total,mean
R3973 T4747 T4745 arg1Of total,band
R3974 T4747 T4746 arg1Of total,pixel
R3975 T4747 T4748 arg1Of total,for
R3976 T4751 T4748 arg2Of arm,for
R3977 T4751 T4749 arg1Of arm,each
R3978 T4751 T4750 arg1Of arm,treatment
R3979 T4751 T4752 arg2Of arm,divided
R3980 T4757 T4752 arg1Of total,divided
R3981 T4757 T4753 arg2Of total,by
R3982 T4757 T4754 arg1Of total,the
R3983 T4757 T4755 arg1Of total,mean
R3984 T4757 T4756 arg1Of total,pixel
R3985 T4757 T4758 arg1Of total,of
R3986 T4759 T4758 arg2Of samples,of
R3987 T4759 T4760 arg2Of samples,stimulated
R3988 T4759 T4764 arg1Of samples,multiplying
R3989 T4760 T4761 arg1Of stimulated,with
R3990 T4760 T4763 arg1Of stimulated,and
R3991 T4762 T4761 arg2Of LPS,with
R3992 T4764 T4763 arg2Of multiplying,and
R3993 T4764 T4765 arg1Of multiplying,by
R3994 T4766 T4765 arg2Of 100,by
R3995 T4770 T4769 arg1Of stimulation,LPS
R3996 T4770 T4771 arg1Of stimulation,is
R3997 T4770 T4772 arg2Of stimulation,reported
R3998 T4772 T4771 arg2Of reported,is
R3999 T4772 T4773 arg1Of reported,as
R4000 T4772 T4776 arg1Of reported,and
R4001 T4775 T4773 arg2Of %,as
R4002 T4775 T4774 arg1Of %,100
R4003 T4776 T4767 arg1Of and,Thus
R4004 T4776 T4768 arg1Of and,","
R4005 T4777 T4778 arg1Of each,of
R4006 T4777 T4782 arg1Of each,is
R4007 T4777 T4783 arg2Of each,reported
R4008 T4781 T4778 arg2Of arms,of
R4009 T4781 T4779 arg1Of arms,the
R4010 T4781 T4780 arg1Of arms,treatment
R4011 T4783 T4776 arg2Of reported,and
R4012 T4783 T4782 arg2Of reported,is
R4013 T4783 T4784 arg1Of reported,as
R4014 T4786 T4784 arg2Of percent,as
R4015 T4786 T4785 arg1Of percent,a
R4016 T4786 T4787 arg1Of percent,of
R4017 T4789 T4787 arg2Of stimulation,of
R4018 T4789 T4788 arg1Of stimulation,LPS