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PMC:2664230 / 4921-13845 JSONTXT 19 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2794 37-45 VB denotes approved
T2795 46-48 IN denotes by
T2796 49-52 DT denotes the
T2797 53-58 NNP denotes Human
T2798 59-67 NNP denotes Research
T2799 68-79 NNP denotes Protections
T2800 80-87 NNP denotes Program
T2801 88-91 CC denotes and
T2802 92-95 DT denotes the
T2803 96-109 NNP denotes Institutional
T2804 110-116 NNP denotes Review
T2805 117-123 NNP denotes Board.
T2806 124-131 NNP denotes Written
T2807 132-139 NN denotes consent
T2808 140-142 TO denotes to
T2809 143-154 VB denotes participate
T2810 155-157 IN denotes in
T2811 158-161 DT denotes the
T2812 162-167 NN denotes study
T2813 168-171 VB denotes was
T2814 172-180 VB denotes obtained
T2815 181-185 IN denotes from
T2816 186-189 DT denotes all
T2817 190-200 NN denotes volunteers
T2818 201-206 RB denotes prior
T2819 207-209 TO denotes to
T2820 210-215 NN denotes blood
T2821 216-237 JJ denotes donation. Heparinized
T2822 238-249 NN denotes Vacutainers
T2823 250-254 VB denotes were
T2824 255-264 VB denotes purchased
T2825 265-269 IN denotes from
T2826 270-276 NNP denotes Becton
T2827 277-286 NNP denotes Dickinson
T2828 287-288 -LRB- denotes (
T2829 288-291 NNP denotes San
T2830 292-296 NNP denotes Jose
T2831 296-297 -COMMA- denotes ,
T2832 298-300 NNP denotes CA
T2833 300-301 -RRB- denotes )
T2834 303-309 NN denotes 1.5-mL
T2835 310-319 NNP denotes Eppendorf
T2836 320-330 NN denotes centrifuge
T2837 331-336 NN denotes tubes
T2838 337-341 VB denotes were
T2839 342-351 VB denotes purchased
T2840 352-356 IN denotes from
T2841 357-363 NNP denotes Fisher
T2842 364-374 NNP denotes Scientific
T2843 375-376 -LRB- denotes (
T2844 376-385 NNP denotes Pittsburg
T2845 385-386 -COMMA- denotes ,
T2846 387-389 NNP denotes PA
T2847 389-390 -RRB- denotes )
T2848 392-398 NN denotes Hank’s
T2849 399-407 JJ denotes balanced
T2850 408-412 NN denotes salt
T2851 413-421 NN denotes solution
T2852 422-423 -LRB- denotes (
T2853 423-427 NN denotes HBSS
T2854 427-428 -RRB- denotes )
T2855 429-432 CC denotes and
T2856 433-437 NN denotes RPMI
T2857 438-442 CD denotes 1640
T2858 443-447 VB denotes were
T2859 448-456 VB denotes obtained
T2860 457-461 IN denotes from
T2861 462-468 NNP denotes Irvine
T2862 469-479 NNP denotes Scientific
T2863 480-481 -LRB- denotes (
T2864 481-486 NNP denotes Santa
T2865 487-490 NNP denotes Ana
T2866 490-491 -COMMA- denotes ,
T2867 492-494 NNP denotes CA
T2868 494-495 -RRB- denotes )
T2869 497-500 NN denotes LPS
T2870 501-517 NN denotes from Escherichia
T2871 518-531 NN denotes coli serotype
T2872 532-536 CD denotes 0111
T2873 536-537 -COLON- denotes :
T2874 537-539 NN denotes B4
T2875 540-543 CC denotes and
T2876 544-547 NN denotes PTX
T2877 548-552 VB denotes were
T2878 553-562 VB denotes purchased
T2879 563-567 IN denotes from
T2880 568-573 NN denotes Sigma
T2881 574-575 -LRB- denotes (
T2882 575-578 NNP denotes St.
T2883 579-584 NNP denotes Louis
T2884 584-585 -COMMA- denotes ,
T2885 586-588 NN denotes MO
T2886 588-589 -RRB- denotes )
T2887 591-594 DT denotes The
T2888 595-598 NN denotes PKA
T2889 599-608 NN denotes inhibitor
T2890 608-609 -COMMA- denotes ,
T2891 610-612 NN denotes N-
T2892 612-613 -LRB- denotes [
T2893 613-615 CD denotes 2-
T2894 615-616 -LRB- denotes (
T2895 616-617 -LRB- denotes (
T2896 617-632 NN denotes p-bromocinnamyl
T2897 632-633 -RRB- denotes )
T2898 633-638 NN denotes amino
T2899 638-639 -RRB- denotes )
T2900 639-644 NN denotes ethyl
T2901 644-645 -RRB- denotes ]
T2902 645-676 NN denotes -5-isoguinolinesulfonamide-2HCl
T2903 676-677 -RRB- denotes ]
T2904 678-679 -LRB- denotes (
T2905 679-682 NN denotes H89
T2906 682-683 -RRB- denotes )
T2907 683-684 -COMMA- denotes ,
T2908 685-688 VB denotes was
T2909 689-698 VB denotes purchased
T2910 699-703 IN denotes from
T2911 704-714 NN denotes Calbiochem
T2912 715-716 -LRB- denotes (
T2913 716-718 NNP denotes La
T2914 719-724 NNP denotes Jolla
T2915 724-725 -COMMA- denotes ,
T2916 726-728 NNP denotes CA
T2917 728-729 -RRB- denotes )
T2918 731-741 NN denotes Antibodies
T2919 742-745 IN denotes for
T2920 746-760 VB denotes phosphorylated
T2921 761-765 NN denotes CREB
T2922 766-767 -LRB- denotes (
T2923 767-773 NN denotes serine
T2924 774-777 CD denotes 133
T2925 777-778 -RRB- denotes )
T2926 778-779 -COMMA- denotes ,
T2927 780-794 VB denotes phosphorylated
T2928 795-800 NN denotes I-κBα
T2929 801-802 -LRB- denotes (
T2930 802-809 NN denotes serines
T2931 810-812 CD denotes 32
T2932 813-816 CC denotes and
T2933 817-819 CD denotes 36
T2934 819-820 -RRB- denotes )
T2935 820-821 -COMMA- denotes ,
T2936 822-836 VB denotes phosphorylated
T2937 837-842 NN denotes NF-κB
T2938 843-846 NN denotes p65
T2939 847-854 NN denotes subunit
T2940 855-856 -LRB- denotes (
T2941 856-862 NN denotes serine
T2942 863-866 CD denotes 276
T2943 866-867 -RRB- denotes )
T2944 867-868 -COMMA- denotes ,
T2945 869-872 CC denotes and
T2946 873-882 JJ denotes secondary
T2947 883-893 NN denotes antibodies
T2948 894-898 VB denotes were
T2949 899-908 VB denotes purchased
T2950 909-913 IN denotes from
T2951 914-918 NN denotes Cell
T2952 919-928 NN denotes Signaling
T2953 929-930 -LRB- denotes (
T2954 930-937 NNP denotes Beverly
T2955 937-938 -COMMA- denotes ,
T2956 939-941 JJ denotes MA
T2957 941-942 -RRB- denotes )
T2958 942-951 NN denotes . Dextran
T2959 952-956 NN denotes T500
T2960 957-960 CC denotes and
T2961 961-968 NN denotes Percoll
T2962 969-973 VB denotes were
T2963 974-982 VB denotes received
T2964 983-987 IN denotes from
T2965 988-990 NNP denotes GE
T2966 991-1010 NNP denotes Healthcare/Amersham
T2967 1011-1022 NNP denotes Biosciences
T2968 1023-1024 -LRB- denotes (
T2969 1024-1034 NNP denotes Piscataway
T2970 1034-1035 -COMMA- denotes ,
T2971 1036-1038 NNP denotes NJ
T2972 1038-1039 -RRB- denotes )
T2973 1041-1044 DT denotes The
T2974 1045-1058 JJ denotes enzyme-linked
T2975 1059-1072 JJ denotes immunosorbent
T2976 1073-1078 NN denotes assay
T2977 1079-1080 -LRB- denotes (
T2978 1080-1085 NN denotes ELISA
T2979 1085-1086 -RRB- denotes )
T2980 1087-1090 IN denotes for
T2981 1091-1096 NN denotes TNF-α
T2982 1097-1100 VB denotes was
T2983 1101-1109 VB denotes obtained
T2984 1110-1114 IN denotes from
T2985 1115-1125 NNP denotes Quantikine
T2986 1126-1127 NNP denotes R
T2987 1127-1128 CC denotes &
T2988 1128-1129 NNP denotes D
T2989 1130-1137 NNP denotes Systems
T2990 1138-1139 -LRB- denotes (
T2991 1139-1150 NNP denotes Minneapolis
T2992 1150-1151 -COMMA- denotes ,
T2993 1152-1154 NN denotes MN
T2994 1154-1155 -RRB- denotes )
T2995 1155-1169 NN denotes . Tris-glycine
T2996 1170-1173 CC denotes and
T2997 1174-1177 NN denotes DNA
T2998 1178-1189 NN denotes retardation
T2999 1190-1194 NN denotes gels
T3000 1194-1195 -COMMA- denotes ,
T3001 1196-1210 NN denotes nitrocellulose
T3002 1211-1220 NN denotes membranes
T3003 1220-1221 -COMMA- denotes ,
T3004 1222-1225 CC denotes and
T3005 1226-1233 JJ denotes western
T3006 1234-1238 NN denotes blot
T3007 1239-1246 VB denotes running
T3008 1247-1254 NN denotes buffers
T3009 1255-1259 VB denotes were
T3010 1260-1268 VB denotes obtained
T3011 1269-1273 IN denotes from
T3012 1274-1284 NN denotes Invitrogen
T3013 1285-1286 -LRB- denotes (
T3014 1286-1294 NNP denotes Carlsbad
T3015 1294-1295 -COMMA- denotes ,
T3016 1296-1298 NN denotes CA
T3017 1298-1299 -RRB- denotes )
T3018 1301-1316 JJ denotes NE-PER® nuclear
T3019 1317-1320 CC denotes and
T3020 1321-1332 JJ denotes cytoplasmic
T3021 1333-1343 NN denotes extraction
T3022 1344-1352 NN denotes reagents
T3023 1352-1353 -COMMA- denotes ,
T3024 1354-1357 DT denotes the
T3025 1358-1361 NN denotes BCA
T3026 1362-1369 NN denotes protein
T3027 1370-1377 NN denotes reagent
T3028 1378-1381 NN denotes kit
T3029 1381-1382 -COMMA- denotes ,
T3030 1383-1386 DT denotes the
T3031 1387-1398 NNP denotes Supersignal
T3032 1399-1403 NNP denotes West
T3033 1404-1408 NNP denotes Pico
T3034 1409-1425 NNP denotes Chemiluminescent
T3035 1426-1429 NNP denotes Kit
T3036 1429-1430 -COMMA- denotes ,
T3037 1431-1434 CC denotes and
T3038 1435-1438 DT denotes the
T3039 1439-1449 NNP denotes LightShift
T3040 1450-1466 NNP denotes Chemiluminescent
T3041 1467-1471 NNP denotes EMSA
T3042 1472-1475 NN denotes Kit
T3043 1476-1480 VB denotes were
T3044 1481-1490 VB denotes purchased
T3045 1491-1495 IN denotes from
T3046 1496-1502 NNP denotes Pierce
T3047 1503-1504 -LRB- denotes (
T3048 1504-1512 NNP denotes Rockland
T3049 1512-1513 -COMMA- denotes ,
T3050 1514-1516 NN denotes IL
T3051 1516-1517 -RRB- denotes )
T3052 1519-1523 NN denotes CREB
T3053 1524-1527 CC denotes and
T3054 1528-1533 NN denotes NF-κB
T3055 1534-1537 NN denotes DNA
T3056 1538-1553 NN denotes oligonucleotide
T3057 1554-1560 NN denotes probes
T3058 1561-1565 VB denotes were
T3059 1566-1577 VB denotes synthesized
T3060 1578-1580 IN denotes by
T3061 1581-1584 NN denotes IDT
T3062 1585-1586 -LRB- denotes (
T3063 1586-1596 NNP denotes Coralville
T3064 1596-1597 -COMMA- denotes ,
T3065 1598-1600 NNP denotes IA
T3066 1600-1601 -RRB- denotes )
T3067 1603-1608 NN denotes Nylon
T3068 1609-1618 NN denotes membranes
T3069 1619-1623 VB denotes were
T3070 1624-1633 VB denotes purchased
T3071 1634-1638 IN denotes from
T3072 1639-1644 NNP denotes Roche
T3073 1645-1652 NNP denotes Applied
T3074 1653-1661 NNP denotes Sciences
T3075 1662-1663 -LRB- denotes (
T3076 1663-1675 NNP denotes Indianapolis
T3077 1675-1676 -COMMA- denotes ,
T3078 1677-1679 NNP denotes IN
T3079 1679-1680 -RRB- denotes )
T3080 1682-1689 NN denotes Western
T3081 1690-1694 NN denotes blot
T3082 1695-1699 NN denotes band
T3083 1700-1714 NN denotes quantification
T3084 1715-1718 VB denotes was
T3085 1719-1728 VB denotes performed
T3086 1729-1733 IN denotes with
T3087 1734-1737 DT denotes the
T3088 1738-1748 NNP denotes UN-SCAN-IT
T3089 1749-1752 NNP denotes Gel
T3090 1753-1763 NNP denotes Digitizing
T3091 1764-1772 NN denotes software
T3092 1773-1774 -LRB- denotes (
T3093 1774-1778 NNP denotes Silk
T3094 1779-1789 NNP denotes Scientific
T3095 1789-1790 -COMMA- denotes ,
T3096 1791-1795 NNP denotes Orem
T3097 1795-1796 -COMMA- denotes ,
T3098 1797-1799 NN denotes UT
T3099 1799-1800 -RRB- denotes )
T3577 1803-1808 JJ denotes Human
T3578 1809-1819 NNP denotes Peripheral
T3579 1820-1825 NNP denotes Blood
T3580 1826-1837 NNP denotes Mononuclear
T3581 1838-1842 NNP denotes Cell
T3582 1843-1852 NN denotes Isolation
T3583 1853-1856 CC denotes and
T3584 1857-1868 NN denotes Stimulation
T3585 1869-1874 JJ denotes Human
T3586 1875-1886 JJ denotes mononuclear
T3587 1887-1892 NN denotes cells
T3588 1893-1897 VB denotes were
T3589 1898-1906 VB denotes isolated
T3590 1907-1911 IN denotes from
T3591 1912-1915 DT denotes the
T3592 1916-1926 JJ denotes peripheral
T3593 1927-1932 NN denotes blood
T3594 1933-1935 IN denotes of
T3595 1936-1940 CD denotes four
T3596 1941-1948 JJ denotes healthy
T3597 1949-1954 JJ denotes human
T3598 1955-1965 NN denotes volunteers
T3599 1967-1970 DT denotes The
T3600 1971-1977 NN denotes sample
T3601 1978-1982 NN denotes size
T3602 1983-1986 VB denotes was
T3603 1987-1993 VB denotes chosen
T3604 1994-1998 IN denotes from
T3605 1999-2002 PRP-DOLLAR- denotes our
T3606 2003-2013 RB denotes previously
T3607 2014-2023 VB denotes published
T3608 2024-2028 NN denotes work
T3609 2028-2029 -COMMA- denotes ,
T3610 2030-2035 WDT denotes which
T3611 2036-2048 VB denotes demonstrated
T3612 2049-2057 JJ denotes reliable
T3613 2058-2068 NN denotes results.10
T3614 2069-2073 NN denotes Cell
T3615 2074-2083 NN denotes isolation
T3616 2084-2087 CC denotes and
T3617 2088-2091 DT denotes all
T3618 2092-2102 JJ denotes subsequent
T3619 2103-2114 NN denotes experiments
T3620 2115-2119 VB denotes were
T3621 2120-2129 VB denotes conducted
T3622 2130-2135 IN denotes under
T3623 2136-2143 JJ denotes sterile
T3624 2144-2147 CC denotes and
T3625 2148-2160 JJ denotes pyrogen-free
T3626 2161-2171 NN denotes conditions
T3627 2173-2178 NN denotes Blood
T3628 2179-2188 VB denotes collected
T3629 2189-2191 IN denotes in
T3630 2192-2199 NN denotes heparin
T3631 2200-2205 NN denotes tubes
T3632 2206-2209 VB denotes was
T3633 2210-2219 VB denotes incubated
T3634 2220-2224 IN denotes with
T3635 2225-2232 NN denotes Dextran
T3636 2233-2237 NN denotes T500
T3637 2238-2241 CC denotes and
T3638 2242-2245 DT denotes the
T3639 2246-2249 JJ denotes red
T3640 2250-2255 NN denotes cells
T3641 2256-2260 VB denotes were
T3642 2261-2271 VB denotes sedimented
T3643 2272-2275 IN denotes for
T3644 2276-2278 CD denotes 40
T3645 2279-2286 NN denotes minutes
T3646 2287-2289 IN denotes at
T3647 2290-2294 NN denotes room
T3648 2295-2306 NN denotes temperature
T3649 2308-2311 DT denotes The
T3650 2312-2321 JJ denotes resultant
T3651 2322-2327 NN denotes serum
T3652 2328-2331 VB denotes was
T3653 2332-2336 RB denotes then
T3654 2337-2343 VB denotes washed
T3655 2344-2348 IN denotes with
T3656 2349-2353 NN denotes HBSS
T3657 2354-2357 CC denotes and
T3658 2358-2367 VB denotes separated
T3659 2368-2370 IN denotes by
T3660 2371-2378 NNP denotes Percoll
T3661 2379-2387 NN denotes gradient
T3662 2388-2402 NN denotes centrifugation
T3663 2403-2412 VB denotes according
T3664 2413-2415 TO denotes to
T3665 2416-2419 DT denotes the
T3666 2420-2434 NN denotes manufacturer’s
T3667 2435-2447 NN denotes instructions
T3668 2449-2453 NN denotes Cell
T3669 2454-2463 NN denotes viability
T3670 2464-2467 VB denotes was
T3671 2468-2476 VB denotes assessed
T3672 2477-2479 IN denotes by
T3673 2480-2486 NN denotes trypan
T3674 2487-2491 JJ denotes blue
T3675 2492-2495 NN denotes dye
T3676 2496-2505 NN denotes exclusion
T3677 2505-2506 -COMMA- denotes ,
T3678 2507-2511 IN denotes with
T3679 2512-2518 NN denotes purity
T3680 2519-2521 IN denotes of
T3681 2522-2529 JJ denotes greater
T3682 2530-2534 IN denotes than
T3683 2535-2537 CD denotes 95
T3684 2537-2538 NN denotes %
T3685 2540-2548 VB denotes Isolated
T3686 2549-2554 NN denotes cells
T3687 2555-2559 VB denotes were
T3688 2560-2571 VB denotes resuspended
T3689 2572-2574 IN denotes in
T3690 2575-2579 NN denotes RPMI
T3691 2580-2584 CD denotes 1640
T3692 2585-2597 VB denotes supplemented
T3693 2598-2602 IN denotes with
T3694 2603-2605 CD denotes 10
T3695 2605-2606 NN denotes %
T3696 2607-2610 NN denotes FBS
T3697 2611-2614 CC denotes and
T3698 2615-2616 CD denotes 5
T3699 2617-2619 NN denotes mM
T3700 2620-2625 NN denotes HEPES
T3701 2626-2628 IN denotes at
T3702 2629-2630 DT denotes a
T3703 2631-2644 NN denotes concentration
T3704 2645-2647 IN denotes of
T3705 2648-2649 CD denotes 1
T3706 2650-2651 SYM denotes x
T3707 2652-2663 NN denotes 107cells/mL
T3708 2665-2669 DT denotes Each
T3709 2670-2680 JJ denotes subsequent
T3710 2681-2691 NN denotes experiment
T3711 2692-2698 VB denotes listed
T3712 2699-2704 IN denotes below
T3713 2705-2708 VB denotes was
T3714 2709-2718 VB denotes conducted
T3715 2719-2721 IN denotes on
T3716 2722-2729 NN denotes samples
T3717 2730-2732 IN denotes of
T3718 2733-2734 CD denotes 5
T3719 2735-2736 NN denotes x
T3720 2737-2740 CD denotes 106
T3721 2741-2746 NN denotes cells
T3722 2747-2750 IN denotes per
T3723 2751-2760 NN denotes treatment
T3724 2761-2766 NN denotes group
T3725 2768-2776 VB denotes Isolated
T3726 2777-2782 NN denotes cells
T3727 2783-2787 VB denotes were
T3728 2788-2798 VB denotes stimulated
T3729 2799-2803 IN denotes with
T3730 2804-2810 CC denotes either
T3731 2811-2815 NN denotes HBSS
T3732 2816-2818 IN denotes as
T3733 2819-2820 DT denotes a
T3734 2821-2829 JJ denotes negative
T3735 2830-2837 NN denotes control
T3736 2837-2838 -COMMA- denotes ,
T3737 2839-2842 NN denotes LPS
T3738 2843-2844 -LRB- denotes (
T3739 2844-2845 CD denotes 1
T3740 2846-2851 NN denotes μg/mL
T3741 2851-2852 -RRB- denotes )
T3742 2852-2853 -COMMA- denotes ,
T3743 2854-2857 NN denotes PTX
T3744 2858-2859 -LRB- denotes (
T3745 2859-2861 CD denotes 20
T3746 2862-2864 NN denotes mM
T3747 2864-2865 -RRB- denotes )
T3748 2865-2866 -COMMA- denotes ,
T3749 2867-2869 CC denotes or
T3750 2870-2881 JJ denotes concomitant
T3751 2882-2885 NN denotes LPS
T3752 2886-2887 SYM denotes /
T3753 2888-2891 NN denotes PTX
T3754 2891-2892 -COMMA- denotes ,
T3755 2893-2895 IN denotes at
T3756 2896-2899 DT denotes the
T3757 2900-2915 JJ denotes above-mentioned
T3758 2916-2930 NN denotes concentrations
T3759 2930-2931 -COMMA- denotes ,
T3760 2932-2935 IN denotes for
T3761 2936-2938 CD denotes 30
T3762 2939-2946 NN denotes minutes
T3763 2947-2949 IN denotes at
T3764 2950-2954 NN denotes 37ºC
T3765 2956-2961 NN denotes Cells
T3766 2962-2966 VB denotes were
T3767 2967-2973 VB denotes placed
T3768 2974-2976 IN denotes on
T3769 2977-2980 NN denotes ice
T3770 2981-2984 IN denotes for
T3771 2985-2987 CD denotes 10
T3772 2988-2995 NN denotes minutes
T3773 2996-2998 TO denotes to
T3774 2999-3003 VB denotes stop
T3775 3004-3007 DT denotes the
T3776 3008-3016 NN denotes reaction
T3777 3017-3020 CC denotes and
T3778 3021-3024 DT denotes the
T3779 3025-3032 NN denotes samples
T3780 3033-3037 VB denotes were
T3781 3038-3042 RB denotes then
T3782 3043-3049 VB denotes stored
T3783 3050-3052 IN denotes at
T3784 3053-3058 NN denotes −70°C
T3785 3059-3062 IN denotes for
T3786 3063-3070 JJ denotes further
T3787 3071-3079 NN denotes analysis
T3788 3081-3084 DT denotes The
T3789 3085-3096 NN denotes stimulation
T3790 3097-3102 NN denotes times
T3791 3103-3106 CC denotes and
T3792 3107-3121 NN denotes concentrations
T3793 3122-3124 IN denotes of
T3794 3125-3128 NN denotes LPS
T3795 3129-3132 CC denotes and
T3796 3133-3136 NN denotes PTX
T3797 3137-3145 VB denotes utilized
T3798 3146-3148 IN denotes in
T3799 3149-3153 DT denotes this
T3800 3154-3159 NN denotes study
T3801 3160-3164 VB denotes were
T3802 3165-3175 VB denotes determined
T3803 3176-3178 IN denotes in
T3804 3179-3187 JJ denotes previous
T3805 3188-3193 NN denotes pilot
T3806 3194-3201 NN denotes studies
T3807 3202-3206 VB denotes done
T3808 3207-3209 IN denotes in
T3809 3210-3213 PRP-DOLLAR- denotes our
T3810 3214-3224 NN denotes laboratory
T3811 3224-3225 -COMMA- denotes ,
T3812 3226-3231 WDT denotes which
T3813 3232-3240 VB denotes examined
T3814 3241-3244 DT denotes the
T3815 3245-3252 NN denotes effects
T3816 3253-3255 IN denotes of
T3817 3256-3266 VB denotes increasing
T3818 3267-3281 NN denotes concentrations
T3819 3282-3284 IN denotes of
T3820 3285-3288 NN denotes PTX
T3821 3289-3291 IN denotes on
T3822 3292-3303 JJ denotes LPS-induced
T3823 3304-3309 NN denotes TNF-α
T3824 3310-3320 NN denotes production
T3825 3322-3325 NN denotes LPS
T3826 3326-3328 IN denotes at
T3827 3329-3330 DT denotes a
T3828 3331-3344 NN denotes concentration
T3829 3345-3347 IN denotes of
T3830 3348-3349 CD denotes 1
T3831 3350-3355 NN denotes μg/mL
T3832 3356-3359 CC denotes and
T3833 3360-3363 NN denotes PTX
T3834 3364-3366 IN denotes at
T3835 3367-3368 DT denotes a
T3836 3369-3382 NN denotes concentration
T3837 3383-3385 IN denotes of
T3838 3386-3388 CD denotes 20
T3839 3389-3391 NN denotes mM
T3840 3392-3396 VB denotes were
T3841 3397-3400 DT denotes the
T3842 3401-3408 JJ denotes minimum
T3843 3409-3423 NN denotes concentrations
T3844 3424-3433 JJ denotes necessary
T3845 3434-3436 TO denotes to
T3846 3437-3444 VB denotes produce
T3847 3445-3446 DT denotes a
T3848 3447-3459 JJ denotes reproducible
T3849 3460-3463 CC denotes and
T3850 3464-3472 JJ denotes reliable
T3851 3473-3486 NN denotes up-regulation
T3852 3487-3490 CC denotes and
T3853 3491-3506 NN denotes down-regulation
T3854 3507-3509 IN denotes in
T3855 3510-3515 NN denotes TNF-α
T3856 3516-3526 NN denotes production
T3857 3526-3527 -COMMA- denotes ,
T3858 3528-3540 RB denotes respectively
T3859 3540-3541 -COMMA- denotes ,
T3860 3542-3544 IN denotes in
T3861 3545-3557 JJ denotes quantitative
T3862 3558-3564 NN denotes assays
T3863 3565-3566 -LRB- denotes (
T3864 3566-3570 NN denotes data
T3865 3571-3574 RB denotes not
T3866 3575-3580 VB denotes shown
T3867 3580-3581 -RRB- denotes )
T4023 3584-3590 NN denotes Effect
T4024 3591-3593 IN denotes of
T4025 3594-3597 NN denotes PTX
T4026 3598-3600 IN denotes on
T4027 3601-3612 JJ denotes LPS-induced
T4028 3613-3618 NN denotes TNF-α
T4029 3619-3629 NN denotes production
T4030 3630-3632 IN denotes by
T4031 3633-3644 JJ denotes mononuclear
T4032 3645-3650 NN denotes cells
T4033 3651-3654 DT denotes The
T4034 3655-3668 NN denotes concentration
T4035 3669-3671 IN denotes of
T4036 3672-3677 NN denotes TNF-α
T4037 3678-3681 VB denotes was
T4038 3682-3696 RB denotes quantitatively
T4039 3697-3707 VB denotes determined
T4040 3708-3710 IN denotes by
T4041 3711-3716 NN denotes ELISA
T4042 3717-3719 IN denotes in
T4043 3720-3723 DT denotes the
T4044 3724-3729 NN denotes media
T4045 3730-3732 IN denotes of
T4046 3733-3738 NN denotes cells
T4047 3739-3746 VB denotes exposed
T4048 3747-3749 TO denotes to
T4049 3750-3753 DT denotes the
T4050 3754-3764 NN denotes treatments
T4051 3765-3774 VB denotes described
T4052 3775-3780 IN denotes above
T4053 3781-3786 IN denotes after
T4054 3787-3798 NN denotes stimulation
T4055 3799-3802 IN denotes for
T4056 3803-3805 RB denotes up
T4057 3806-3808 TO denotes to
T4058 3809-3810 DT denotes a
T4059 3811-3818 NN denotes maximum
T4060 3819-3821 IN denotes of
T4061 3822-3824 CD denotes 18
T4062 3825-3830 NN denotes hours
T4063 3830-3831 -COMMA- denotes ,
T4064 3832-3834 IN denotes at
T4065 3835-3841 JJ denotes 3-hour
T4066 3842-3851 NN denotes intervals
T4067 3853-3856 DT denotes The
T4068 3857-3864 NN denotes results
T4069 3865-3868 VB denotes are
T4070 3869-3878 VB denotes expressed
T4071 3879-3881 IN denotes in
T4072 3882-3887 NN denotes pg/mL
T4475 3890-3898 NN denotes SDS-PAGE
T4476 3899-3902 CC denotes and
T4477 3903-3917 NN denotes Immunoblotting
T4478 3918-3919 -LRB- denotes (
T4479 3919-3924 NN denotes I-κBα
T4480 3924-3925 -COMMA- denotes ,
T4481 3926-3931 NN denotes NF-κB
T4482 3932-3935 NN denotes p65
T4483 3935-3936 -COMMA- denotes ,
T4484 3937-3940 CC denotes and
T4485 3941-3945 NN denotes CREB
T4486 3945-3946 -RRB- denotes )
T4487 3947-3956 VB denotes Following
T4488 3957-3968 NN denotes stimulation
T4489 3968-3969 -COMMA- denotes ,
T4490 3970-3975 NN denotes PBMCs
T4491 3976-3980 VB denotes were
T4492 3981-3987 VB denotes washed
T4493 3988-3992 IN denotes with
T4494 3993-4001 JJ denotes ice-cold
T4495 4002-4005 NN denotes PBS
T4496 4006-4009 CC denotes and
T4497 4010-4021 VB denotes centrifuged
T4498 4022-4024 TO denotes to
T4499 4025-4032 VB denotes collect
T4500 4033-4034 DT denotes a
T4501 4035-4039 NN denotes cell
T4502 4040-4046 NN denotes pellet
T4503 4048-4051 DT denotes The
T4504 4052-4058 NN denotes pellet
T4505 4059-4062 VB denotes was
T4506 4063-4074 VB denotes resuspended
T4507 4075-4077 IN denotes in
T4508 4078-4086 JJ denotes ice-cold
T4509 4087-4090 NN denotes SDS
T4510 4091-4097 NN denotes sample
T4511 4098-4104 NN denotes buffer
T4512 4105-4117 VB denotes supplemented
T4513 4118-4122 IN denotes with
T4514 4123-4126 CD denotes 100
T4515 4127-4129 NN denotes mM
T4516 4130-4144 NN denotes dithiothreitol
T4517 4146-4157 JJ denotes Cytoplasmic
T4518 4158-4161 CC denotes and
T4519 4162-4169 JJ denotes nuclear
T4520 4170-4178 NN denotes extracts
T4521 4179-4183 VB denotes were
T4522 4184-4192 VB denotes isolated
T4523 4193-4197 IN denotes with
T4524 4198-4204 NN denotes NE-PER
T4525 4205-4212 JJ denotes nuclear
T4526 4213-4216 CC denotes and
T4527 4217-4228 JJ denotes cytoplasmic
T4528 4229-4239 NN denotes extraction
T4529 4240-4248 NN denotes reagents
T4530 4249-4253 IN denotes with
T4531 4254-4256 NN denotes 1x
T4532 4257-4261 NNP denotes Halt
T4533 4262-4270 NNP denotes Protease
T4534 4271-4280 NNP denotes Inhibitor
T4535 4281-4289 NNP denotes Cocktail
T4536 4290-4299 VB denotes according
T4537 4300-4302 TO denotes to
T4538 4303-4306 DT denotes the
T4539 4307-4321 NN denotes manufacturer’s
T4540 4322-4334 NN denotes instructions
T4541 4336-4343 NN denotes Protein
T4542 4344-4358 NN denotes concentrations
T4543 4359-4363 VB denotes were
T4544 4364-4374 VB denotes determined
T4545 4375-4379 IN denotes with
T4546 4380-4383 DT denotes the
T4547 4384-4387 NN denotes BCA
T4548 4388-4395 NN denotes protein
T4549 4396-4403 NN denotes reagent
T4550 4404-4407 NN denotes kit
T4551 4408-4411 IN denotes for
T4552 4412-4416 DT denotes each
T4553 4417-4423 NN denotes sample
T4554 4424-4433 VB denotes according
T4555 4434-4436 TO denotes to
T4556 4437-4438 DT denotes a
T4557 4439-4451 JJ denotes standardized
T4558 4452-4457 NN denotes curve
T4559 4458-4461 IN denotes for
T4560 4462-4469 NN denotes albumin
T4561 4471-4475 NN denotes Cell
T4562 4476-4483 NN denotes lysates
T4563 4484-4488 VB denotes were
T4564 4489-4498 VB denotes collected
T4565 4499-4501 IN denotes by
T4566 4502-4509 VB denotes boiling
T4567 4510-4513 DT denotes the
T4568 4514-4521 NN denotes samples
T4569 4522-4524 IN denotes in
T4570 4525-4526 DT denotes a
T4571 4527-4532 NN denotes 100ºC
T4572 4533-4538 NN denotes water
T4573 4539-4543 NN denotes bath
T4574 4544-4547 IN denotes for
T4575 4548-4549 CD denotes 5
T4576 4550-4557 NN denotes minutes
T4577 4559-4562 CD denotes Ten
T4578 4563-4565 NN denotes μg
T4579 4566-4568 IN denotes of
T4580 4569-4576 NN denotes protein
T4581 4577-4580 IN denotes per
T4582 4581-4587 NN denotes sample
T4583 4588-4591 VB denotes was
T4584 4592-4601 VB denotes separated
T4585 4602-4604 IN denotes by
T4586 4605-4623 NN denotes SDS-polyacrylamide
T4587 4624-4627 NN denotes gel
T4588 4628-4643 NN denotes electrophoresis
T4589 4644-4651 IN denotes through
T4590 4652-4656 CD denotes 8–16
T4591 4656-4657 NN denotes %
T4592 4658-4670 NN denotes tris-glycine
T4593 4671-4685 NN denotes polyacrylamide
T4594 4686-4694 NN denotes gradient
T4595 4695-4699 NN denotes gels
T4596 4700-4703 CC denotes and
T4597 4704-4715 VB denotes transferred
T4598 4716-4718 TO denotes to
T4599 4719-4733 VB denotes nitrocellulose
T4600 4734-4743 NN denotes membranes
T4601 4745-4748 DT denotes The
T4602 4749-4758 NN denotes membranes
T4603 4759-4763 VB denotes were
T4604 4764-4771 VB denotes blocked
T4605 4772-4776 IN denotes with
T4606 4777-4778 CD denotes 5
T4607 4778-4779 NN denotes %
T4608 4780-4784 NN denotes milk
T4609 4785-4787 IN denotes in
T4610 4788-4801 JJ denotes Tris-buffered
T4611 4802-4814 NN denotes saline/Tween
T4612 4815-4817 CD denotes 20
T4613 4818-4819 -LRB- denotes (
T4614 4819-4826 NNP denotes Fischer
T4615 4827-4837 NNP denotes Scientific
T4616 4837-4838 -COMMA- denotes ,
T4617 4839-4849 NNP denotes Pittsburgh
T4618 4849-4850 -COMMA- denotes ,
T4619 4851-4853 NNP denotes PA
T4620 4853-4854 -RRB- denotes )
T4621 4855-4858 IN denotes for
T4622 4859-4860 CD denotes 1
T4623 4861-4865 NN denotes hour
T4624 4867-4878 JJ denotes Cytoplasmic
T4625 4879-4887 NN denotes extracts
T4626 4888-4892 VB denotes were
T4627 4893-4902 VB denotes incubated
T4628 4903-4907 IN denotes with
T4629 4908-4922 VB denotes phosphorylated
T4630 4923-4928 JJ denotes I-κBα
T4631 4929-4937 NN denotes antibody
T4632 4938-4939 -LRB- denotes (
T4633 4939-4940 CD denotes 1
T4634 4940-4941 -COLON- denotes :
T4635 4941-4944 CD denotes 200
T4636 4944-4945 -RRB- denotes )
T4637 4945-4946 -COMMA- denotes ,
T4638 4947-4952 IN denotes while
T4639 4953-4960 JJ denotes nuclear
T4640 4961-4969 NN denotes extracts
T4641 4970-4974 VB denotes were
T4642 4975-4984 VB denotes incubated
T4643 4985-4989 IN denotes with
T4644 4990-4996 CC denotes either
T4645 4997-5011 VB denotes phosphorylated
T4646 5012-5017 NN denotes NF-κB
T4647 5018-5021 NN denotes p65
T4648 5022-5030 NN denotes antibody
T4649 5031-5032 -LRB- denotes (
T4650 5032-5033 CD denotes 1
T4651 5033-5034 -COLON- denotes :
T4652 5034-5037 CD denotes 500
T4653 5037-5038 -RRB- denotes )
T4654 5039-5041 CC denotes or
T4655 5042-5056 VB denotes phosphorylated
T4656 5057-5061 NN denotes CREB
T4657 5062-5070 NN denotes antibody
T4658 5071-5072 -LRB- denotes (
T4659 5072-5073 CD denotes 1
T4660 5073-5074 -COLON- denotes :
T4661 5074-5077 CD denotes 500
T4662 5077-5078 -RRB- denotes )
T4663 5079-5088 RB denotes overnight
T4664 5089-5091 IN denotes at
T4665 5092-5095 NN denotes 4°C
T4666 5096-5098 IN denotes in
T4667 5099-5107 JJ denotes separate
T4668 5108-5119 NN denotes experiments
T4669 5121-5124 DT denotes The
T4670 5125-5134 NN denotes membranes
T4671 5135-5139 VB denotes were
T4672 5140-5146 VB denotes washed
T4673 5147-5151 IN denotes with
T4674 5152-5165 JJ denotes Tris-buffered
T4675 5166-5178 NN denotes saline/Tween
T4676 5179-5181 CD denotes 20
T4677 5182-5185 CC denotes and
T4678 5186-5195 VB denotes incubated
T4679 5196-5199 IN denotes for
T4680 5200-5201 CD denotes 1
T4681 5202-5206 NN denotes hour
T4682 5207-5209 IN denotes at
T4683 5210-5214 NN denotes room
T4684 5215-5226 NN denotes temperature
T4685 5227-5231 IN denotes with
T4686 5232-5235 DT denotes the
T4687 5236-5245 JJ denotes secondary
T4688 5246-5254 NN denotes antibody
T4689 5254-5255 -COMMA- denotes ,
T4690 5256-5267 JJ denotes horseradish
T4691 5268-5285 JJ denotes peroxidase-linked
T4692 5286-5297 JJ denotes anti-rabbit
T4693 5298-5301 NN denotes IgG
T4694 5302-5309 VB denotes diluted
T4695 5310-5311 CD denotes 1
T4696 5311-5312 -COLON- denotes :
T4697 5312-5316 CD denotes 2000
T4698 5317-5319 IN denotes in
T4699 5320-5328 VB denotes blocking
T4700 5329-5337 NN denotes solution
T4701 5339-5344 IN denotes After
T4702 5345-5353 VB denotes repeated
T4703 5354-5361 NN denotes washing
T4704 5362-5364 IN denotes of
T4705 5365-5368 DT denotes the
T4706 5369-5377 NN denotes membrane
T4707 5377-5378 -COMMA- denotes ,
T4708 5379-5382 DT denotes the
T4709 5383-5394 NNP denotes Supersignal
T4710 5395-5399 NNP denotes West
T4711 5400-5404 NNP denotes Pico
T4712 5405-5421 NNP denotes Chemiluminescent
T4713 5422-5425 NNP denotes Kit
T4714 5426-5429 VB denotes was
T4715 5430-5437 VB denotes applied
T4716 5438-5441 IN denotes for
T4717 5442-5450 NN denotes antibody
T4718 5451-5460 NN denotes detection
T4719 5461-5464 IN denotes per
T4720 5465-5468 DT denotes the
T4721 5469-5483 NN denotes manufacturer’s
T4722 5484-5496 NN denotes instructions
T4723 5498-5505 JJ denotes Western
T4724 5506-5510 NN denotes blot
T4725 5511-5515 NN denotes data
T4726 5516-5518 VB denotes is
T4727 5519-5528 VB denotes presented
T4728 5529-5531 IN denotes as
T4729 5532-5533 DT denotes a
T4730 5534-5544 NN denotes percentage
T4731 5545-5547 IN denotes of
T4732 5548-5551 NN denotes LPS
T4733 5552-5563 NN denotes stimulation
T4734 5565-5568 DT denotes The
T4735 5569-5579 NN denotes percentage
T4736 5580-5582 IN denotes of
T4737 5583-5586 NN denotes LPS
T4738 5587-5598 NN denotes stimulation
T4739 5599-5602 VB denotes was
T4740 5603-5613 VB denotes calculated
T4741 5614-5616 IN denotes by
T4742 5617-5625 VB denotes dividing
T4743 5626-5629 DT denotes the
T4744 5630-5634 JJ denotes mean
T4745 5635-5639 NN denotes band
T4746 5640-5645 NN denotes pixel
T4747 5646-5651 NN denotes total
T4748 5652-5655 IN denotes for
T4749 5656-5660 DT denotes each
T4750 5661-5670 NN denotes treatment
T4751 5671-5674 NN denotes arm
T4752 5675-5682 VB denotes divided
T4753 5683-5685 IN denotes by
T4754 5686-5689 DT denotes the
T4755 5690-5694 JJ denotes mean
T4756 5695-5700 NN denotes pixel
T4757 5701-5706 NN denotes total
T4758 5707-5709 IN denotes of
T4759 5710-5717 NN denotes samples
T4760 5718-5728 VB denotes stimulated
T4761 5729-5733 IN denotes with
T4762 5734-5737 NN denotes LPS
T4763 5738-5741 CC denotes and
T4764 5742-5753 VB denotes multiplying
T4765 5754-5756 IN denotes by
T4766 5757-5760 CD denotes 100
T4767 5762-5766 RB denotes Thus
T4768 5766-5767 -COMMA- denotes ,
T4769 5768-5771 NN denotes LPS
T4770 5772-5783 NN denotes stimulation
T4771 5784-5786 VB denotes is
T4772 5787-5795 VB denotes reported
T4773 5796-5798 IN denotes as
T4774 5799-5802 CD denotes 100
T4775 5802-5803 NN denotes %
T4776 5804-5807 CC denotes and
T4777 5808-5812 DT denotes each
T4778 5813-5815 IN denotes of
T4779 5816-5819 DT denotes the
T4780 5820-5829 NN denotes treatment
T4781 5830-5834 NN denotes arms
T4782 5835-5837 VB denotes is
T4783 5838-5846 VB denotes reported
T4784 5847-5849 IN denotes as
T4785 5850-5851 DT denotes a
T4786 5852-5859 NN denotes percent
T4787 5860-5862 IN denotes of
T4788 5863-5866 NN denotes LPS
T4789 5867-5878 NN denotes stimulation
T5231 5881-5886 NN denotes NF-κB
T5232 5887-5890 CC denotes and
T5233 5891-5895 NN denotes CREB
T5234 5896-5911 JJ denotes Electrophoretic
T5235 5912-5920 NN denotes Mobility
T5236 5921-5926 NN denotes Shift
T5237 5927-5932 NN denotes Assay
T5238 5933-5934 -LRB- denotes (
T5239 5934-5938 NN denotes EMSA
T5240 5938-5939 -RRB- denotes )
T5241 5940-5943 DT denotes The
T5242 5944-5959 JJ denotes non-radioactive
T5243 5960-5970 NNP denotes LightShift
T5244 5971-5987 NNP denotes Chemiluminescent
T5245 5988-5992 NNP denotes EMSA
T5246 5993-5996 NN denotes Kit
T5247 5997-6000 VB denotes was
T5248 6001-6005 VB denotes used
T5249 6006-6008 TO denotes to
T5250 6009-6015 VB denotes detect
T5251 6016-6027 JJ denotes DNA-protein
T5252 6028-6040 NN denotes interactions
T5253 6042-6045 DT denotes The
T5254 6046-6051 NN denotes NF-κB
T5255 6052-6054 NN denotes 3′
T5256 6055-6061 NN denotes biotin
T5257 6062-6073 JJ denotes end-labeled
T5258 6074-6077 NN denotes DNA
T5259 6078-6093 NN denotes oligonucleotide
T5260 6094-6098 VB denotes used
T5261 6099-6101 IN denotes as
T5262 6102-6103 DT denotes a
T5263 6104-6109 NN denotes probe
T5264 6110-6113 IN denotes for
T5265 6114-6117 DT denotes the
T5266 6118-6122 NN denotes EMSA
T5267 6123-6126 VB denotes was
T5268 6127-6128 DT denotes a
T5269 6129-6134 JJ denotes 42-bp
T5270 6135-6141 JJ denotes double
T5271 6142-6150 JJ denotes stranded
T5272 6151-6160 NN denotes construct
T5273 6161-6162 -LRB- denotes (
T5274 6162-6208 NN denotes 5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
T5275 6209-6210 NN denotes –
T5276 6211-6213 NN denotes 3′
T5277 6213-6214 -RRB- denotes )
T5278 6215-6225 VB denotes containing
T5279 6226-6229 CD denotes two
T5280 6230-6238 RB denotes tandemly
T5281 6239-6247 VB denotes repeated
T5282 6248-6253 NN denotes NF-κB
T5283 6254-6261 NN denotes binding
T5284 6262-6267 NN denotes sites
T5285 6268-6269 -LRB- denotes (
T5286 6269-6279 VB denotes underlined
T5287 6279-6280 -RRB- denotes )
T5288 6282-6293 NN denotes Specificity
T5289 6294-6297 VB denotes was
T5290 6298-6308 VB denotes determined
T5291 6309-6311 IN denotes by
T5292 6312-6313 DT denotes a
T5293 6314-6325 NN denotes competition
T5294 6326-6331 NN denotes assay
T5295 6332-6336 IN denotes with
T5296 6337-6340 DT denotes the
T5297 6341-6349 NN denotes addition
T5298 6350-6352 IN denotes of
T5299 6353-6356 CD denotes 200
T5300 6357-6362 JJ denotes molar
T5301 6363-6369 NN denotes excess
T5302 6370-6372 IN denotes of
T5303 6373-6382 JJ denotes unlabeled
T5304 6383-6389 JJ denotes double
T5305 6390-6398 JJ denotes stranded
T5306 6399-6404 NN denotes NF-κB
T5307 6405-6420 NN denotes oligonucleotide
T5308 6422-6425 DT denotes The
T5309 6426-6430 NN denotes CREB
T5310 6431-6433 NN denotes 3′
T5311 6434-6440 NN denotes biotin
T5312 6441-6452 JJ denotes end-labeled
T5313 6453-6456 NN denotes DNA
T5314 6457-6472 NN denotes oligonucleotide
T5315 6473-6477 VB denotes used
T5316 6478-6480 IN denotes as
T5317 6481-6482 DT denotes a
T5318 6483-6488 NN denotes probe
T5319 6489-6492 IN denotes for
T5320 6493-6496 DT denotes the
T5321 6497-6501 NN denotes EMSA
T5322 6502-6505 VB denotes was
T5323 6506-6507 DT denotes a
T5324 6508-6513 JJ denotes 23-bp
T5325 6514-6520 JJ denotes double
T5326 6521-6529 JJ denotes stranded
T5327 6530-6539 NN denotes construct
T5328 6540-6541 -LRB- denotes (
T5329 6541-6547 NN denotes 5′-TTT
T5330 6548-6551 NN denotes TCG
T5331 6552-6555 NN denotes AGC
T5332 6556-6571 NN denotes TCTGACGTCAGA-GC
T5333 6572-6573 NN denotes –
T5334 6574-6576 NN denotes 3′
T5335 6576-6577 -RRB- denotes )
T5336 6578-6588 VB denotes containing
T5337 6589-6592 DT denotes the
T5338 6593-6596 NN denotes CRE
T5339 6597-6606 NN denotes consensus
T5340 6607-6615 NN denotes sequence
T5341 6616-6617 -LRB- denotes (
T5342 6617-6627 VB denotes underlined
T5343 6627-6628 -RRB- denotes )
T5344 6630-6641 NN denotes Specificity
T5345 6642-6645 VB denotes was
T5346 6646-6656 VB denotes determined
T5347 6657-6659 IN denotes by
T5348 6660-6661 DT denotes a
T5349 6662-6673 NN denotes competition
T5350 6674-6679 NN denotes assay
T5351 6680-6684 IN denotes with
T5352 6685-6688 DT denotes the
T5353 6689-6697 NN denotes addition
T5354 6698-6700 IN denotes of
T5355 6701-6704 CD denotes 200
T5356 6705-6710 JJ denotes molar
T5357 6711-6717 NN denotes excess
T5358 6718-6720 IN denotes of
T5359 6721-6730 JJ denotes unlabeled
T5360 6731-6737 JJ denotes double
T5361 6738-6746 JJ denotes stranded
T5362 6747-6751 NN denotes CREB
T5363 6752-6767 NN denotes oligonucleotide
T5364 6769-6776 JJ denotes Nuclear
T5365 6777-6785 NN denotes extracts
T5366 6786-6787 -LRB- denotes (
T5367 6787-6789 CD denotes 10
T5368 6790-6792 NN denotes μg
T5369 6792-6793 -RRB- denotes )
T5370 6794-6798 VB denotes were
T5371 6799-6808 VB denotes incubated
T5372 6809-6813 IN denotes with
T5373 6814-6815 CD denotes 5
T5374 6816-6818 NN denotes nM
T5375 6819-6824 NN denotes NF-κB
T5376 6825-6827 CC denotes or
T5377 6828-6832 NN denotes CREB
T5378 6833-6838 NN denotes probe
T5379 6839-6840 -LRB- denotes (
T5380 6840-6845 NN denotes NF-kB
T5381 6845-6846 -COLON- denotes :
T5382 6847-6849 NN denotes 1x
T5383 6850-6857 NN denotes binding
T5384 6858-6864 NN denotes buffer
T5385 6864-6865 -COMMA- denotes ,
T5386 6866-6868 CD denotes 50
T5387 6869-6871 NN denotes mM
T5388 6872-6875 NN denotes KCl
T5389 6875-6876 -COMMA- denotes ,
T5390 6877-6878 CD denotes 1
T5391 6879-6881 NN denotes mM
T5392 6882-6886 NN denotes EDTA
T5393 6886-6887 -COMMA- denotes ,
T5394 6888-6889 CD denotes 1
T5395 6890-6892 NN denotes mM
T5396 6893-6896 NN denotes DTT
T5397 6896-6897 -COMMA- denotes ,
T5398 6898-6901 CD denotes 0.1
T5399 6901-6902 NN denotes %
T5400 6903-6907 NN denotes NP40
T5401 6907-6908 -COMMA- denotes ,
T5402 6909-6911 CD denotes 10
T5403 6911-6912 NN denotes %
T5404 6913-6921 NN denotes glycerol
T5405 6921-6922 -COMMA- denotes ,
T5406 6923-6926 CC denotes and
T5407 6927-6929 CD denotes 50
T5408 6930-6935 NN denotes ng/μl
T5409 6936-6940 NN denotes poly
T5410 6941-6946 NN denotes dI-dC
T5411 6946-6947 -COMMA- denotes ,
T5412 6948-6952 NN denotes CREB
T5413 6952-6953 -COLON- denotes :
T5414 6954-6956 NN denotes 1x
T5415 6957-6964 NN denotes binding
T5416 6965-6971 NN denotes buffer
T5417 6971-6972 -COMMA- denotes ,
T5418 6973-6975 CD denotes 20
T5419 6976-6978 NN denotes mM
T5420 6979-6983 NN denotes Tris
T5421 6983-6984 -COMMA- denotes ,
T5422 6985-6987 NN denotes pH
T5423 6988-6991 CD denotes 7.5
T5424 6991-6992 -COMMA- denotes ,
T5425 6993-6995 CD denotes 50
T5426 6996-6998 NN denotes mM
T5427 6999-7002 NN denotes KCl
T5428 7002-7003 -COMMA- denotes ,
T5429 7004-7005 CD denotes 1
T5430 7006-7008 NN denotes mM
T5431 7009-7013 NN denotes EDTA
T5432 7013-7014 -COMMA- denotes ,
T5433 7015-7016 CD denotes 1
T5434 7017-7019 NN denotes mM
T5435 7020-7023 NN denotes DTT
T5436 7023-7024 -COMMA- denotes ,
T5437 7025-7029 CD denotes 0.10
T5438 7029-7030 NN denotes %
T5439 7031-7035 NN denotes NP40
T5440 7035-7036 -COMMA- denotes ,
T5441 7037-7038 CD denotes 6
T5442 7038-7039 NN denotes %
T5443 7040-7048 NN denotes glycerol
T5444 7048-7049 -COMMA- denotes ,
T5445 7050-7053 CD denotes 0.1
T5446 7054-7059 NN denotes mg/mL
T5447 7060-7063 NN denotes BSA
T5448 7064-7067 CC denotes and
T5449 7068-7070 CD denotes 50
T5450 7071-7076 NN denotes ng/μl
T5451 7077-7081 NN denotes poly
T5452 7082-7087 NN denotes dI-dC
T5453 7087-7088 -RRB- denotes )
T5454 7089-7092 CC denotes and
T5455 7093-7097 VB denotes were
T5456 7098-7102 RB denotes then
T5457 7103-7118 VB denotes electrophoresed
T5458 7119-7126 IN denotes through
T5459 7127-7128 DT denotes a
T5460 7129-7130 CD denotes 6
T5461 7130-7131 NN denotes %
T5462 7132-7135 NN denotes DNA
T5463 7136-7147 NN denotes retardation
T5464 7148-7151 NN denotes gel
T5465 7152-7154 IN denotes at
T5466 7155-7159 NN denotes 100V
T5467 7160-7163 IN denotes for
T5468 7164-7166 CD denotes 90
T5469 7167-7174 NN denotes minutes
T5470 7176-7179 DT denotes The
T5471 7180-7184 NN denotes gels
T5472 7185-7189 VB denotes were
T5473 7190-7209 RB denotes electrophoretically
T5474 7210-7221 VB denotes transferred
T5475 7222-7224 IN denotes at
T5476 7225-7230 NN denotes 380mA
T5477 7231-7234 IN denotes for
T5478 7235-7236 CD denotes 1
T5479 7237-7241 NN denotes hour
T5480 7242-7244 IN denotes on
T5481 7245-7248 NN denotes ice
T5482 7249-7251 TO denotes to
T5483 7252-7253 DT denotes a
T5484 7254-7264 RB denotes positively
T5485 7265-7272 VB denotes charged
T5486 7273-7278 NN denotes nylon
T5487 7279-7287 NN denotes membrane
T5488 7288-7291 CC denotes and
T5489 7292-7303 RB denotes immediately
T5490 7304-7316 JJ denotes cross-linked
T5491 7317-7320 IN denotes for
T5492 7321-7323 CD denotes 15
T5493 7324-7331 NN denotes minutes
T5494 7332-7336 IN denotes with
T5495 7337-7338 DT denotes a
T5496 7339-7341 NN denotes UV
T5497 7342-7358 NN denotes transilluminator
T5498 7359-7367 VB denotes equipped
T5499 7368-7372 IN denotes with
T5500 7373-7374 DT denotes a
T5501 7375-7378 CD denotes 312
T5502 7379-7381 NN denotes nm
T5503 7382-7386 NN denotes bulb
T5504 7388-7412 JJ denotes Streptavidin-horseradish
T5505 7413-7423 NN denotes peroxidase
T5506 7424-7433 NN denotes conjugate
T5507 7434-7437 CC denotes and
T5508 7438-7441 DT denotes the
T5509 7442-7452 NNP denotes LightShift
T5510 7453-7469 NNP denotes Chemiluminescent
T5511 7470-7479 NNP denotes Substrate
T5512 7480-7484 VB denotes were
T5513 7485-7489 VB denotes used
T5514 7490-7492 TO denotes to
T5515 7493-7499 VB denotes detect
T5516 7500-7503 DT denotes the
T5517 7504-7510 NN denotes biotin
T5518 7511-7522 JJ denotes end-labeled
T5519 7523-7526 NN denotes DNA
T5520 7528-7531 DT denotes The
T5521 7532-7537 NN denotes nylon
T5522 7538-7547 NN denotes membranes
T5523 7548-7552 VB denotes were
T5524 7553-7560 VB denotes exposed
T5525 7561-7563 TO denotes to
T5526 7564-7569 NN denotes x-ray
T5527 7570-7574 NN denotes film
T5528 7575-7578 IN denotes for
T5529 7579-7582 CD denotes 1–3
T5530 7583-7590 NN denotes minutes
T5531 7591-7594 IN denotes for
T5532 7595-7604 NN denotes detection
T5799 7607-7611 NN denotes Role
T5800 7612-7614 IN denotes of
T5801 7615-7618 NN denotes PKA
T5802 7619-7621 IN denotes on
T5803 7622-7633 JJ denotes LPS-induced
T5804 7634-7645 JJ denotes mononuclear
T5805 7646-7650 NN denotes cell
T5806 7651-7656 NN denotes TNF-α
T5807 7657-7667 NN denotes production
T5808 7668-7670 TO denotes To
T5809 7671-7679 VB denotes evaluate
T5810 7680-7683 DT denotes the
T5811 7684-7688 NN denotes role
T5812 7689-7691 IN denotes of
T5813 7692-7695 NN denotes PKA
T5814 7696-7698 IN denotes on
T5815 7699-7704 NN denotes TNF-α
T5816 7705-7715 NN denotes production
T5817 7716-7718 IN denotes in
T5818 7719-7730 JJ denotes mononuclear
T5819 7731-7736 NN denotes cells
T5820 7737-7740 CC denotes and
T5821 7741-7744 PRP-DOLLAR- denotes its
T5822 7745-7756 NN denotes involvement
T5823 7757-7759 IN denotes in
T5824 7760-7763 DT denotes the
T5825 7764-7775 NN denotes attenuation
T5826 7776-7778 IN denotes of
T5827 7779-7790 JJ denotes LPS-induced
T5828 7791-7796 NN denotes TNF-α
T5829 7797-7807 NN denotes production
T5830 7808-7816 VB denotes observed
T5831 7817-7826 VB denotes following
T5832 7827-7830 NN denotes PTX
T5833 7831-7840 NN denotes treatment
T5834 7840-7841 -COMMA- denotes ,
T5835 7842-7845 NN denotes H89
T5836 7845-7846 -COMMA- denotes ,
T5837 7847-7848 DT denotes a
T5838 7849-7857 JJ denotes specific
T5839 7858-7861 NN denotes PKA
T5840 7862-7871 NN denotes inhibitor
T5841 7871-7872 -COMMA- denotes ,
T5842 7873-7876 VB denotes was
T5843 7877-7885 VB denotes utilized
T5844 7887-7895 VB denotes Isolated
T5845 7896-7907 JJ denotes mononuclear
T5846 7908-7913 NN denotes cells
T5847 7914-7918 VB denotes were
T5848 7919-7928 VB denotes incubated
T5849 7929-7938 VB denotes according
T5850 7939-7941 TO denotes to
T5851 7942-7945 DT denotes the
T5852 7946-7955 NN denotes treatment
T5853 7956-7962 NN denotes groups
T5854 7963-7972 VB denotes described
T5855 7973-7978 RB denotes above
T5856 7979-7981 IN denotes in
T5857 7982-7985 DT denotes the
T5858 7986-7994 NN denotes presence
T5859 7995-7998 CC denotes and
T5860 7999-8006 NN denotes absence
T5861 8007-8009 IN denotes of
T5862 8010-8022 NN denotes pretreatment
T5863 8023-8027 IN denotes with
T5864 8028-8031 NN denotes H89
T5865 8032-8033 -LRB- denotes (
T5866 8033-8035 CD denotes 10
T5867 8036-8038 NN denotes μM
T5868 8038-8039 -RRB- denotes )
T5869 8040-8043 IN denotes for
T5870 8044-8045 CD denotes 1
T5871 8046-8050 NN denotes hour
T5872 8051-8053 IN denotes at
T5873 8054-8058 NN denotes 37ºC
T5874 8060-8063 DT denotes The
T5875 8064-8068 NN denotes dose
T5876 8069-8071 IN denotes of
T5877 8072-8075 NN denotes H89
T5878 8076-8079 VB denotes was
T5879 8080-8086 VB denotes chosen
T5880 8087-8092 VB denotes based
T5881 8093-8095 IN denotes on
T5882 8096-8104 JJ denotes previous
T5883 8105-8109 NN denotes work
T5884 8110-8115 WDT denotes which
T5885 8116-8128 VB denotes demonstrated
T5886 8129-8137 JJ denotes specific
T5887 8138-8141 CC denotes and
T5888 8142-8150 JJ denotes complete
T5889 8151-8161 NN denotes inhibition
T5890 8162-8164 IN denotes of
T5891 8165-8168 NN denotes PKA
T5892 8169-8171 IN denotes at
T5893 8172-8176 DT denotes this
T5894 8177-8193 NN denotes concentration.10
T5895 8194-8199 IN denotes Since
T5896 8200-8203 DT denotes the
T5897 8204-8212 NN denotes activity
T5898 8213-8215 IN denotes of
T5899 8216-8220 DT denotes this
T5900 8221-8230 NN denotes inhibitor
T5901 8231-8233 VB denotes is
T5902 8234-8238 CC denotes both
T5903 8239-8247 JJ denotes specific
T5904 8248-8251 CC denotes and
T5905 8252-8262 JJ denotes consistent
T5906 8263-8265 IN denotes at
T5907 8266-8270 DT denotes this
T5908 8271-8280 VB denotes specified
T5909 8281-8285 NN denotes dose
T5910 8285-8286 -COMMA- denotes ,
T5911 8287-8289 PRP denotes we
T5912 8290-8293 VB denotes did
T5913 8294-8297 RB denotes not
T5914 8298-8305 VB denotes include
T5915 8306-8317 NN denotes experiments
T5916 8318-8322 IN denotes with
T5917 8323-8333 JJ denotes additional
T5918 8334-8337 NN denotes PKA
T5919 8338-8348 NN denotes inhibitors
T5920 8349-8353 IN denotes with
T5921 8354-8358 DT denotes this
T5922 8359-8362 NN denotes set
T5923 8363-8365 IN denotes of
T5924 8366-8377 NN denotes experiments
T5925 8379-8382 DT denotes The
T5926 8383-8388 NN denotes TNF-α
T5927 8389-8402 NN denotes concentration
T5928 8403-8405 IN denotes in
T5929 8406-8409 DT denotes the
T5930 8410-8421 NN denotes supernatant
T5931 8422-8425 VB denotes was
T5932 8426-8434 VB denotes measured
T5933 8435-8449 RB denotes quantitatively
T5934 8450-8452 IN denotes by
T5935 8453-8458 NN denotes ELISA
T5936 8460-8463 DT denotes The
T5937 8464-8471 NN denotes results
T5938 8472-8475 VB denotes are
T5939 8476-8485 VB denotes expressed
T5940 8486-8488 IN denotes in
T5941 8489-8494 NN denotes pg/mL
T6140 8497-8508 JJ denotes Statistical
T6141 8509-8517 NN denotes Analysis
T6142 8518-8521 DT denotes The
T6143 8522-8534 JJ denotes experimental
T6144 8535-8542 NN denotes results
T6145 8543-8551 VB denotes obtained
T6146 8552-8554 IN denotes in
T6147 8555-8559 DT denotes this
T6148 8560-8565 NN denotes study
T6149 8566-8570 VB denotes were
T6150 8571-8578 VB denotes derived
T6151 8579-8583 IN denotes from
T6152 8584-8588 CD denotes four
T6153 8589-8597 JJ denotes separate
T6154 8598-8609 NN denotes experiments
T6155 8610-8614 IN denotes with
T6156 8615-8622 JJ denotes healthy
T6157 8623-8632 NN denotes volunteer
T6158 8633-8639 NN denotes donors
T6159 8641-8645 DT denotes Each
T6160 8646-8651 NN denotes assay
T6161 8652-8655 VB denotes was
T6162 8656-8665 VB denotes performed
T6163 8666-8668 IN denotes in
T6164 8669-8678 NN denotes duplicate
T6165 8679-8681 CC denotes or
T6166 8682-8692 VB denotes triplicate
T6167 8693-8698 WRB denotes where
T6168 8699-8710 JJ denotes appropriate
T6169 8712-8716 NN denotes Data
T6170 8717-8719 VB denotes is
T6171 8720-8729 VB denotes presented
T6172 8730-8732 IN denotes as
T6173 8733-8736 DT denotes the
T6174 8737-8741 JJ denotes mean
T6175 8742-8743 NN denotes ±
T6176 8744-8747 NN denotes SEM
T6177 8749-8760 JJ denotes Statistical
T6178 8761-8772 NN denotes differences
T6179 8773-8780 IN denotes between
T6180 8781-8787 NN denotes groups
T6181 8788-8792 VB denotes were
T6182 8793-8803 VB denotes determined
T6183 8804-8806 IN denotes by
T6184 8807-8814 JJ denotes one-way
T6185 8815-8823 NN denotes analysis
T6186 8824-8826 IN denotes of
T6187 8827-8835 NN denotes variance
T6188 8836-8837 -LRB- denotes (
T6189 8837-8842 NN denotes ANOVA
T6190 8842-8843 -RRB- denotes )
T6191 8844-8848 IN denotes with
T6192 8849-8850 DT denotes a
T6193 8851-8861 NNP denotes Bonferroni
T6194 8862-8872 NN denotes correction
T6195 8874-8885 JJ denotes Statistical
T6196 8886-8898 NN denotes significance
T6197 8899-8902 VB denotes was
T6198 8903-8910 VB denotes defined
T6199 8911-8913 IN denotes as
T6200 8914-8915 NN denotes P
T6201 8916-8917 SYM denotes <
T6202 8918-8922 CD denotes 0.05
R2222 T2794 T2810 arg1Of approved,in
R2223 T2800 T2796 arg1Of Program,the
R2224 T2800 T2797 arg1Of Program,Human
R2225 T2800 T2798 arg1Of Program,Research
R2226 T2800 T2799 arg1Of Program,Protections
R2227 T2800 T2801 arg1Of Program,and
R2228 T2801 T2794 arg1Of and,approved
R2229 T2801 T2795 arg2Of and,by
R2230 T2806 T2803 arg1Of Written,Institutional
R2231 T2806 T2804 arg1Of Written,Review
R2232 T2806 T2805 arg1Of Written,Board.
R2233 T2807 T2801 arg2Of consent,and
R2234 T2807 T2802 arg1Of consent,the
R2235 T2807 T2806 arg1Of consent,Written
R2236 T2807 T2808 modOf consent,to
R2237 T2809 T2808 arg1Of participate,to
R2238 T2812 T2811 arg1Of study,the
R2239 T2812 T2813 arg1Of study,was
R2240 T2812 T2814 arg2Of study,obtained
R2241 T2814 T2813 arg2Of obtained,was
R2242 T2814 T2815 arg1Of obtained,from
R2243 T2814 T2818 arg1Of obtained,prior
R2244 T2817 T2815 arg2Of volunteers,from
R2245 T2817 T2816 arg1Of volunteers,all
R2246 T2818 T2819 arg1Of prior,to
R2247 T2820 T2819 arg2Of blood,to
R2248 T2822 T2821 arg1Of Vacutainers,"donation. Heparinized"
R2249 T2822 T2823 arg1Of Vacutainers,were
R2250 T2822 T2824 arg2Of Vacutainers,purchased
R2251 T2824 T2810 arg2Of purchased,in
R2252 T2824 T2813 modOf purchased,was
R2253 T2824 T2823 arg2Of purchased,were
R2254 T2824 T2825 arg1Of purchased,from
R2255 T2827 T2825 arg2Of Dickinson,from
R2256 T2827 T2826 arg1Of Dickinson,Becton
R2257 T2827 T2828 arg1Of Dickinson,(
R2258 T2830 T2828 arg2Of Jose,(
R2259 T2830 T2829 arg1Of Jose,San
R2260 T2830 T2831 arg1Of Jose,","
R2261 T2832 T2831 arg2Of CA,","
R2262 T2833 T2828 arg3Of ),(
R2263 T2837 T2834 arg1Of tubes,1.5-mL
R2264 T2837 T2835 arg1Of tubes,Eppendorf
R2265 T2837 T2836 arg1Of tubes,centrifuge
R2266 T2837 T2838 arg1Of tubes,were
R2267 T2837 T2839 arg2Of tubes,purchased
R2268 T2839 T2838 arg2Of purchased,were
R2269 T2839 T2840 arg1Of purchased,from
R2270 T2842 T2840 arg2Of Scientific,from
R2271 T2842 T2841 arg1Of Scientific,Fisher
R2272 T2842 T2843 arg1Of Scientific,(
R2273 T2844 T2843 arg2Of Pittsburg,(
R2274 T2844 T2845 arg1Of Pittsburg,","
R2275 T2844 T2846 arg1Of Pittsburg,PA
R2276 T2847 T2843 arg3Of ),(
R2277 T2851 T2848 arg1Of solution,Hank’s
R2278 T2851 T2849 arg1Of solution,balanced
R2279 T2851 T2850 arg1Of solution,salt
R2280 T2851 T2852 arg1Of solution,(
R2281 T2851 T2855 arg1Of solution,and
R2282 T2853 T2852 arg2Of HBSS,(
R2283 T2854 T2852 arg3Of ),(
R2284 T2855 T2858 arg1Of and,were
R2285 T2855 T2859 arg2Of and,obtained
R2286 T2856 T2855 arg2Of RPMI,and
R2287 T2856 T2857 arg1Of RPMI,1640
R2288 T2859 T2858 arg2Of obtained,were
R2289 T2859 T2860 arg1Of obtained,from
R2290 T2862 T2860 arg2Of Scientific,from
R2291 T2862 T2861 arg1Of Scientific,Irvine
R2292 T2862 T2863 arg1Of Scientific,(
R2293 T2865 T2863 arg2Of Ana,(
R2294 T2865 T2864 arg1Of Ana,Santa
R2295 T2865 T2866 arg1Of Ana,","
R2296 T2867 T2866 arg2Of CA,","
R2297 T2868 T2863 arg3Of ),(
R2298 T2871 T2869 arg1Of coli serotype,LPS
R2299 T2871 T2870 arg1Of coli serotype,from Escherichia
R2300 T2871 T2872 arg1Of coli serotype,0111
R2301 T2871 T2873 arg1Of coli serotype,:
R2302 T2871 T2877 arg1Of coli serotype,were
R2303 T2871 T2878 arg2Of coli serotype,purchased
R2304 T2874 T2875 arg1Of B4,and
R2305 T2875 T2873 arg2Of and,:
R2306 T2876 T2875 arg2Of PTX,and
R2307 T2878 T2877 arg2Of purchased,were
R2308 T2878 T2879 arg1Of purchased,from
R2309 T2880 T2879 arg2Of Sigma,from
R2310 T2880 T2881 arg1Of Sigma,(
R2311 T2883 T2881 arg2Of Louis,(
R2312 T2883 T2882 arg1Of Louis,St.
R2313 T2883 T2884 arg1Of Louis,","
R2314 T2885 T2884 arg2Of MO,","
R2315 T2886 T2881 arg3Of ),(
R2316 T2889 T2887 arg1Of inhibitor,The
R2317 T2889 T2888 arg1Of inhibitor,PKA
R2318 T2889 T2890 arg1Of inhibitor,","
R2319 T2891 T2890 arg2Of N-,","
R2320 T2891 T2892 arg1Of N-,[
R2321 T2893 T2892 arg2Of 2-,[
R2322 T2893 T2894 arg1Of 2-,(
R2323 T2893 T2900 arg1Of 2-,ethyl
R2324 T2896 T2895 arg2Of p-bromocinnamyl,(
R2325 T2897 T2895 arg3Of ),(
R2326 T2898 T2894 arg2Of amino,(
R2327 T2898 T2895 arg1Of amino,(
R2328 T2899 T2894 arg3Of ),(
R2329 T2901 T2892 arg3Of ],[
R2330 T2902 T2889 arg1Of -5-isoguinolinesulfonamide-2HCl,inhibitor
R2331 T2902 T2903 arg1Of -5-isoguinolinesulfonamide-2HCl,]
R2332 T2902 T2904 arg1Of -5-isoguinolinesulfonamide-2HCl,(
R2333 T2902 T2908 arg1Of -5-isoguinolinesulfonamide-2HCl,was
R2334 T2902 T2909 arg2Of -5-isoguinolinesulfonamide-2HCl,purchased
R2335 T2905 T2904 arg2Of H89,(
R2336 T2906 T2904 arg3Of ),(
R2337 T2909 T2907 arg1Of purchased,","
R2338 T2909 T2908 arg2Of purchased,was
R2339 T2909 T2910 arg1Of purchased,from
R2340 T2911 T2910 arg2Of Calbiochem,from
R2341 T2911 T2912 arg1Of Calbiochem,(
R2342 T2914 T2912 arg2Of Jolla,(
R2343 T2914 T2913 arg1Of Jolla,La
R2344 T2914 T2915 arg1Of Jolla,","
R2345 T2916 T2915 arg2Of CA,","
R2346 T2917 T2912 arg3Of ),(
R2347 T2918 T2919 arg1Of Antibodies,for
R2348 T2918 T2945 arg1Of Antibodies,and
R2349 T2921 T2920 arg2Of CREB,phosphorylated
R2350 T2921 T2922 arg1Of CREB,(
R2351 T2921 T2926 arg1Of CREB,","
R2352 T2923 T2922 arg2Of serine,(
R2353 T2923 T2924 arg1Of serine,133
R2354 T2925 T2922 arg3Of ),(
R2355 T2926 T2935 arg1Of ",",","
R2356 T2928 T2926 arg2Of I-κBα,","
R2357 T2928 T2927 arg2Of I-κBα,phosphorylated
R2358 T2928 T2929 arg1Of I-κBα,(
R2359 T2931 T2932 arg1Of 32,and
R2360 T2932 T2929 arg2Of and,(
R2361 T2932 T2930 arg1Of and,serines
R2362 T2933 T2932 arg2Of 36,and
R2363 T2934 T2929 arg3Of ),(
R2364 T2935 T2919 arg2Of ",",for
R2365 T2939 T2935 arg2Of subunit,","
R2366 T2939 T2936 arg2Of subunit,phosphorylated
R2367 T2939 T2937 arg1Of subunit,NF-κB
R2368 T2939 T2938 arg1Of subunit,p65
R2369 T2939 T2940 arg1Of subunit,(
R2370 T2941 T2940 arg2Of serine,(
R2371 T2941 T2942 arg1Of serine,276
R2372 T2943 T2940 arg3Of ),(
R2373 T2945 T2944 arg1Of and,","
R2374 T2945 T2948 arg1Of and,were
R2375 T2945 T2949 arg2Of and,purchased
R2376 T2947 T2945 arg2Of antibodies,and
R2377 T2947 T2946 arg1Of antibodies,secondary
R2378 T2949 T2948 arg2Of purchased,were
R2379 T2949 T2950 arg1Of purchased,from
R2380 T2949 T2953 arg1Of purchased,(
R2381 T2952 T2950 arg2Of Signaling,from
R2382 T2952 T2951 arg1Of Signaling,Cell
R2383 T2954 T2953 arg2Of Beverly,(
R2384 T2954 T2955 arg1Of Beverly,","
R2385 T2954 T2956 arg1Of Beverly,MA
R2386 T2957 T2953 arg3Of ),(
R2387 T2959 T2960 arg1Of T500,and
R2388 T2960 T2958 arg1Of and,". Dextran"
R2389 T2960 T2962 arg1Of and,were
R2390 T2960 T2963 arg2Of and,received
R2391 T2961 T2960 arg2Of Percoll,and
R2392 T2963 T2948 modOf received,were
R2393 T2963 T2962 arg2Of received,were
R2394 T2963 T2964 arg1Of received,from
R2395 T2967 T2964 arg2Of Biosciences,from
R2396 T2967 T2965 arg1Of Biosciences,GE
R2397 T2967 T2966 arg1Of Biosciences,Healthcare/Amersham
R2398 T2967 T2968 arg1Of Biosciences,(
R2399 T2969 T2968 arg2Of Piscataway,(
R2400 T2969 T2970 arg1Of Piscataway,","
R2401 T2969 T2971 arg1Of Piscataway,NJ
R2402 T2972 T2968 arg3Of ),(
R2403 T2976 T2973 arg1Of assay,The
R2404 T2976 T2974 arg1Of assay,enzyme-linked
R2405 T2976 T2975 arg1Of assay,immunosorbent
R2406 T2976 T2977 arg1Of assay,(
R2407 T2976 T2980 arg1Of assay,for
R2408 T2976 T2982 arg1Of assay,was
R2409 T2976 T2983 arg2Of assay,obtained
R2410 T2978 T2977 arg2Of ELISA,(
R2411 T2979 T2977 arg3Of ),(
R2412 T2981 T2980 arg2Of TNF-α,for
R2413 T2983 T2982 arg2Of obtained,was
R2414 T2983 T2984 arg1Of obtained,from
R2415 T2983 T3004 arg1Of obtained,and
R2416 T2989 T2985 arg1Of Systems,Quantikine
R2417 T2989 T2986 arg1Of Systems,R
R2418 T2989 T2987 arg1Of Systems,&
R2419 T2989 T2988 arg1Of Systems,D
R2420 T2989 T2990 arg1Of Systems,(
R2421 T2991 T2990 arg2Of Minneapolis,(
R2422 T2991 T2992 arg1Of Minneapolis,","
R2423 T2991 T2993 arg1Of Minneapolis,MN
R2424 T2994 T2990 arg3Of ),(
R2425 T2995 T2989 arg1Of ". Tris-glycine",Systems
R2426 T2995 T2996 arg1Of ". Tris-glycine",and
R2427 T2996 T3000 arg1Of and,","
R2428 T2999 T2996 arg2Of gels,and
R2429 T2999 T2997 arg1Of gels,DNA
R2430 T2999 T2998 arg1Of gels,retardation
R2431 T3000 T2984 arg2Of ",",from
R2432 T3002 T3000 arg2Of membranes,","
R2433 T3002 T3001 arg1Of membranes,nitrocellulose
R2434 T3004 T3003 arg1Of and,","
R2435 T3008 T3005 arg1Of buffers,western
R2436 T3008 T3006 arg1Of buffers,blot
R2437 T3008 T3007 arg1Of buffers,running
R2438 T3008 T3009 arg1Of buffers,were
R2439 T3008 T3010 arg2Of buffers,obtained
R2440 T3010 T3004 arg2Of obtained,and
R2441 T3010 T3009 arg2Of obtained,were
R2442 T3010 T3011 arg1Of obtained,from
R2443 T3010 T3013 arg1Of obtained,(
R2444 T3012 T3011 arg2Of Invitrogen,from
R2445 T3014 T3013 arg2Of Carlsbad,(
R2446 T3014 T3015 arg1Of Carlsbad,","
R2447 T3014 T3016 arg1Of Carlsbad,CA
R2448 T3017 T3013 arg3Of ),(
R2449 T3018 T3019 arg1Of NE-PER® nuclear,and
R2450 T3020 T3019 arg2Of cytoplasmic,and
R2451 T3022 T3018 arg1Of reagents,NE-PER® nuclear
R2452 T3022 T3020 arg1Of reagents,cytoplasmic
R2453 T3022 T3021 arg1Of reagents,extraction
R2454 T3022 T3023 arg1Of reagents,","
R2455 T3023 T3029 arg1Of ",",","
R2456 T3023 T3037 arg1Of ",",and
R2457 T3028 T3023 arg2Of kit,","
R2458 T3028 T3024 arg1Of kit,the
R2459 T3028 T3025 arg1Of kit,BCA
R2460 T3028 T3026 arg1Of kit,protein
R2461 T3028 T3027 arg1Of kit,reagent
R2462 T3035 T3029 arg2Of Kit,","
R2463 T3035 T3030 arg1Of Kit,the
R2464 T3035 T3031 arg1Of Kit,Supersignal
R2465 T3035 T3032 arg1Of Kit,West
R2466 T3035 T3033 arg1Of Kit,Pico
R2467 T3035 T3034 arg1Of Kit,Chemiluminescent
R2468 T3037 T3036 arg1Of and,","
R2469 T3037 T3043 arg1Of and,were
R2470 T3037 T3044 arg2Of and,purchased
R2471 T3041 T3039 arg1Of EMSA,LightShift
R2472 T3041 T3040 arg1Of EMSA,Chemiluminescent
R2473 T3042 T3037 arg2Of Kit,and
R2474 T3042 T3038 arg1Of Kit,the
R2475 T3042 T3041 arg1Of Kit,EMSA
R2476 T3044 T3043 arg2Of purchased,were
R2477 T3044 T3045 arg1Of purchased,from
R2478 T3046 T3045 arg2Of Pierce,from
R2479 T3046 T3047 arg1Of Pierce,(
R2480 T3048 T3047 arg2Of Rockland,(
R2481 T3048 T3049 arg1Of Rockland,","
R2482 T3050 T3049 arg2Of IL,","
R2483 T3051 T3047 arg3Of ),(
R2484 T3052 T3053 arg1Of CREB,and
R2485 T3054 T3053 arg2Of NF-κB,and
R2486 T3057 T3052 arg1Of probes,CREB
R2487 T3057 T3054 arg1Of probes,NF-κB
R2488 T3057 T3055 arg1Of probes,DNA
R2489 T3057 T3056 arg1Of probes,oligonucleotide
R2490 T3057 T3058 arg1Of probes,were
R2491 T3057 T3059 arg2Of probes,synthesized
R2492 T3059 T3058 arg2Of synthesized,were
R2493 T3059 T3062 arg1Of synthesized,(
R2494 T3061 T3059 arg1Of IDT,synthesized
R2495 T3061 T3060 arg2Of IDT,by
R2496 T3063 T3062 arg2Of Coralville,(
R2497 T3063 T3064 arg1Of Coralville,","
R2498 T3063 T3065 arg1Of Coralville,IA
R2499 T3066 T3062 arg3Of ),(
R2500 T3068 T3067 arg1Of membranes,Nylon
R2501 T3068 T3069 arg1Of membranes,were
R2502 T3068 T3070 arg2Of membranes,purchased
R2503 T3070 T3069 arg2Of purchased,were
R2504 T3070 T3071 arg1Of purchased,from
R2505 T3074 T3071 arg2Of Sciences,from
R2506 T3074 T3072 arg1Of Sciences,Roche
R2507 T3074 T3073 arg1Of Sciences,Applied
R2508 T3074 T3075 arg1Of Sciences,(
R2509 T3076 T3075 arg2Of Indianapolis,(
R2510 T3076 T3077 arg1Of Indianapolis,","
R2511 T3076 T3078 arg1Of Indianapolis,IN
R2512 T3079 T3075 arg3Of ),(
R2513 T3083 T3080 arg1Of quantification,Western
R2514 T3083 T3081 arg1Of quantification,blot
R2515 T3083 T3082 arg1Of quantification,band
R2516 T3083 T3084 arg1Of quantification,was
R2517 T3083 T3085 arg2Of quantification,performed
R2518 T3085 T3084 arg2Of performed,was
R2519 T3085 T3086 arg1Of performed,with
R2520 T3090 T3088 arg1Of Digitizing,UN-SCAN-IT
R2521 T3090 T3089 arg1Of Digitizing,Gel
R2522 T3091 T3086 arg2Of software,with
R2523 T3091 T3087 arg1Of software,the
R2524 T3091 T3090 arg1Of software,Digitizing
R2525 T3091 T3092 arg1Of software,(
R2526 T3094 T3093 arg1Of Scientific,Silk
R2527 T3094 T3095 arg1Of Scientific,","
R2528 T3095 T3092 arg2Of ",",(
R2529 T3095 T3097 arg1Of ",",","
R2530 T3096 T3095 arg2Of Orem,","
R2531 T3098 T3097 arg2Of UT,","
R2532 T3099 T3092 arg3Of ),(
R2910 T3581 T3578 arg1Of Cell,Peripheral
R2911 T3581 T3579 arg1Of Cell,Blood
R2912 T3581 T3580 arg1Of Cell,Mononuclear
R2913 T3582 T3583 arg1Of Isolation,and
R2914 T3583 T3577 arg1Of and,Human
R2915 T3583 T3581 arg1Of and,Cell
R2916 T3584 T3583 arg2Of Stimulation,and
R2917 T3587 T3585 arg1Of cells,Human
R2918 T3587 T3586 arg1Of cells,mononuclear
R2919 T3587 T3588 arg1Of cells,were
R2920 T3587 T3589 arg2Of cells,isolated
R2921 T3589 T3588 arg2Of isolated,were
R2922 T3589 T3590 arg1Of isolated,from
R2923 T3593 T3590 arg2Of blood,from
R2924 T3593 T3591 arg1Of blood,the
R2925 T3593 T3592 arg1Of blood,peripheral
R2926 T3593 T3594 arg1Of blood,of
R2927 T3598 T3594 arg2Of volunteers,of
R2928 T3598 T3595 arg1Of volunteers,four
R2929 T3598 T3596 arg1Of volunteers,healthy
R2930 T3598 T3597 arg1Of volunteers,human
R2931 T3601 T3599 arg1Of size,The
R2932 T3601 T3600 arg1Of size,sample
R2933 T3601 T3602 arg1Of size,was
R2934 T3601 T3603 arg2Of size,chosen
R2935 T3603 T3602 arg2Of chosen,was
R2936 T3603 T3604 arg1Of chosen,from
R2937 T3603 T3616 arg1Of chosen,and
R2938 T3607 T3606 arg1Of published,previously
R2939 T3608 T3604 arg2Of work,from
R2940 T3608 T3605 arg1Of work,our
R2941 T3608 T3607 arg1Of work,published
R2942 T3608 T3609 arg1Of work,","
R2943 T3608 T3610 arg1Of work,which
R2944 T3608 T3611 arg1Of work,demonstrated
R2945 T3615 T3611 arg2Of isolation,demonstrated
R2946 T3615 T3612 arg1Of isolation,reliable
R2947 T3615 T3613 arg1Of isolation,results.10
R2948 T3615 T3614 arg1Of isolation,Cell
R2949 T3619 T3617 arg1Of experiments,all
R2950 T3619 T3618 arg1Of experiments,subsequent
R2951 T3619 T3620 arg1Of experiments,were
R2952 T3619 T3621 arg2Of experiments,conducted
R2953 T3621 T3616 arg2Of conducted,and
R2954 T3621 T3620 arg2Of conducted,were
R2955 T3621 T3622 arg1Of conducted,under
R2956 T3623 T3624 arg1Of sterile,and
R2957 T3625 T3624 arg2Of pyrogen-free,and
R2958 T3626 T3622 arg2Of conditions,under
R2959 T3626 T3623 arg1Of conditions,sterile
R2960 T3626 T3625 arg1Of conditions,pyrogen-free
R2961 T3627 T3628 arg2Of Blood,collected
R2962 T3627 T3632 arg1Of Blood,was
R2963 T3627 T3633 arg2Of Blood,incubated
R2964 T3628 T3629 arg1Of collected,in
R2965 T3631 T3629 arg2Of tubes,in
R2966 T3631 T3630 arg1Of tubes,heparin
R2967 T3633 T3632 arg2Of incubated,was
R2968 T3633 T3634 arg1Of incubated,with
R2969 T3633 T3637 arg1Of incubated,and
R2970 T3636 T3634 arg2Of T500,with
R2971 T3636 T3635 arg1Of T500,Dextran
R2972 T3640 T3638 arg1Of cells,the
R2973 T3640 T3639 arg1Of cells,red
R2974 T3640 T3641 arg1Of cells,were
R2975 T3640 T3642 arg2Of cells,sedimented
R2976 T3642 T3637 arg2Of sedimented,and
R2977 T3642 T3641 arg2Of sedimented,were
R2978 T3642 T3643 arg1Of sedimented,for
R2979 T3645 T3643 arg2Of minutes,for
R2980 T3645 T3644 arg1Of minutes,40
R2981 T3645 T3646 arg1Of minutes,at
R2982 T3648 T3646 arg2Of temperature,at
R2983 T3648 T3647 arg1Of temperature,room
R2984 T3651 T3649 arg1Of serum,The
R2985 T3651 T3650 arg1Of serum,resultant
R2986 T3651 T3652 arg1Of serum,was
R2987 T3651 T3654 arg2Of serum,washed
R2988 T3651 T3658 arg2Of serum,separated
R2989 T3654 T3655 arg1Of washed,with
R2990 T3654 T3657 arg1Of washed,and
R2991 T3656 T3655 arg2Of HBSS,with
R2992 T3657 T3652 arg2Of and,was
R2993 T3657 T3653 arg1Of and,then
R2994 T3658 T3657 arg2Of separated,and
R2995 T3658 T3663 arg1Of separated,according
R2996 T3662 T3658 arg1Of centrifugation,separated
R2997 T3662 T3659 arg2Of centrifugation,by
R2998 T3662 T3660 arg1Of centrifugation,Percoll
R2999 T3662 T3661 arg1Of centrifugation,gradient
R3000 T3664 T3663 arg2Of to,according
R3001 T3667 T3664 arg2Of instructions,to
R3002 T3667 T3665 arg1Of instructions,the
R3003 T3667 T3666 arg1Of instructions,manufacturer’s
R3004 T3669 T3668 arg1Of viability,Cell
R3005 T3669 T3670 arg1Of viability,was
R3006 T3669 T3671 arg2Of viability,assessed
R3007 T3671 T3670 arg2Of assessed,was
R3008 T3671 T3677 arg1Of assessed,","
R3009 T3671 T3678 arg1Of assessed,with
R3010 T3676 T3671 arg1Of exclusion,assessed
R3011 T3676 T3672 arg2Of exclusion,by
R3012 T3676 T3673 arg1Of exclusion,trypan
R3013 T3676 T3674 arg1Of exclusion,blue
R3014 T3676 T3675 arg1Of exclusion,dye
R3015 T3679 T3678 arg2Of purity,with
R3016 T3679 T3680 arg1Of purity,of
R3017 T3681 T3682 arg1Of greater,than
R3018 T3683 T3682 arg2Of 95,than
R3019 T3684 T3680 arg2Of %,of
R3020 T3684 T3681 arg1Of %,greater
R3021 T3686 T3685 arg2Of cells,Isolated
R3022 T3686 T3687 arg1Of cells,were
R3023 T3686 T3688 arg2Of cells,resuspended
R3024 T3688 T3687 arg2Of resuspended,were
R3025 T3688 T3689 arg1Of resuspended,in
R3026 T3688 T3701 arg1Of resuspended,at
R3027 T3690 T3689 arg2Of RPMI,in
R3028 T3690 T3691 arg1Of RPMI,1640
R3029 T3690 T3692 arg2Of RPMI,supplemented
R3030 T3692 T3693 arg1Of supplemented,with
R3031 T3696 T3694 arg1Of FBS,10
R3032 T3696 T3695 arg1Of FBS,%
R3033 T3696 T3697 arg1Of FBS,and
R3034 T3697 T3693 arg2Of and,with
R3035 T3698 T3699 arg1Of 5,mM
R3036 T3700 T3697 arg2Of HEPES,and
R3037 T3700 T3698 arg1Of HEPES,5
R3038 T3703 T3701 arg2Of concentration,at
R3039 T3703 T3702 arg1Of concentration,a
R3040 T3703 T3704 arg1Of concentration,of
R3041 T3707 T3704 arg2Of 107cells/mL,of
R3042 T3707 T3705 arg1Of 107cells/mL,1
R3043 T3707 T3706 arg1Of 107cells/mL,x
R3044 T3710 T3708 arg1Of experiment,Each
R3045 T3710 T3709 arg1Of experiment,subsequent
R3046 T3710 T3711 arg2Of experiment,listed
R3047 T3710 T3713 arg1Of experiment,was
R3048 T3710 T3714 arg2Of experiment,conducted
R3049 T3711 T3712 arg1Of listed,below
R3050 T3714 T3713 arg2Of conducted,was
R3051 T3714 T3715 arg1Of conducted,on
R3052 T3716 T3715 arg2Of samples,on
R3053 T3716 T3717 arg1Of samples,of
R3054 T3721 T3717 arg2Of cells,of
R3055 T3721 T3718 arg1Of cells,5
R3056 T3721 T3719 arg1Of cells,x
R3057 T3721 T3720 arg1Of cells,106
R3058 T3721 T3722 arg1Of cells,per
R3059 T3724 T3722 arg2Of group,per
R3060 T3724 T3723 arg1Of group,treatment
R3061 T3726 T3725 arg2Of cells,Isolated
R3062 T3726 T3727 arg1Of cells,were
R3063 T3726 T3728 arg2Of cells,stimulated
R3064 T3728 T3727 arg2Of stimulated,were
R3065 T3728 T3729 arg1Of stimulated,with
R3066 T3728 T3732 arg1Of stimulated,as
R3067 T3731 T3729 arg2Of HBSS,with
R3068 T3731 T3730 arg1Of HBSS,either
R3069 T3735 T3732 arg2Of control,as
R3070 T3735 T3733 arg1Of control,a
R3071 T3735 T3734 arg1Of control,negative
R3072 T3735 T3736 arg1Of control,","
R3073 T3737 T3738 arg1Of LPS,(
R3074 T3737 T3742 arg1Of LPS,","
R3075 T3740 T3738 arg2Of μg/mL,(
R3076 T3740 T3739 arg1Of μg/mL,1
R3077 T3741 T3738 arg3Of ),(
R3078 T3742 T3749 arg1Of ",",or
R3079 T3743 T3742 arg2Of PTX,","
R3080 T3743 T3744 arg1Of PTX,(
R3081 T3746 T3744 arg2Of mM,(
R3082 T3746 T3745 arg1Of mM,20
R3083 T3747 T3744 arg3Of ),(
R3084 T3749 T3736 arg2Of or,","
R3085 T3749 T3748 arg1Of or,","
R3086 T3749 T3754 arg1Of or,","
R3087 T3749 T3755 arg1Of or,at
R3088 T3749 T3759 arg1Of or,","
R3089 T3749 T3760 arg1Of or,for
R3090 T3753 T3749 arg2Of PTX,or
R3091 T3753 T3750 arg1Of PTX,concomitant
R3092 T3753 T3751 arg1Of PTX,LPS
R3093 T3753 T3752 arg1Of PTX,/
R3094 T3758 T3755 arg2Of concentrations,at
R3095 T3758 T3756 arg1Of concentrations,the
R3096 T3758 T3757 arg1Of concentrations,above-mentioned
R3097 T3762 T3760 arg2Of minutes,for
R3098 T3762 T3761 arg1Of minutes,30
R3099 T3762 T3763 arg1Of minutes,at
R3100 T3764 T3763 arg2Of 37ºC,at
R3101 T3765 T3766 arg1Of Cells,were
R3102 T3765 T3767 arg2Of Cells,placed
R3103 T3767 T3766 arg2Of placed,were
R3104 T3767 T3768 arg1Of placed,on
R3105 T3767 T3777 arg1Of placed,and
R3106 T3769 T3768 arg2Of ice,on
R3107 T3769 T3770 arg1Of ice,for
R3108 T3772 T3770 arg2Of minutes,for
R3109 T3772 T3771 arg1Of minutes,10
R3110 T3772 T3774 arg1Of minutes,stop
R3111 T3774 T3770 arg3Of stop,for
R3112 T3774 T3773 arg1Of stop,to
R3113 T3776 T3774 arg2Of reaction,stop
R3114 T3776 T3775 arg1Of reaction,the
R3115 T3779 T3778 arg1Of samples,the
R3116 T3779 T3780 arg1Of samples,were
R3117 T3779 T3782 arg2Of samples,stored
R3118 T3782 T3777 arg2Of stored,and
R3119 T3782 T3780 arg2Of stored,were
R3120 T3782 T3781 arg1Of stored,then
R3121 T3782 T3783 arg1Of stored,at
R3122 T3784 T3783 arg2Of −70°C,at
R3123 T3784 T3785 arg1Of −70°C,for
R3124 T3787 T3785 arg2Of analysis,for
R3125 T3787 T3786 arg1Of analysis,further
R3126 T3790 T3789 arg1Of times,stimulation
R3127 T3790 T3791 arg1Of times,and
R3128 T3791 T3788 arg1Of and,The
R3129 T3791 T3801 arg1Of and,were
R3130 T3791 T3802 arg2Of and,determined
R3131 T3792 T3791 arg2Of concentrations,and
R3132 T3792 T3793 arg1Of concentrations,of
R3133 T3794 T3795 arg1Of LPS,and
R3134 T3795 T3793 arg2Of and,of
R3135 T3795 T3797 arg2Of and,utilized
R3136 T3796 T3795 arg2Of PTX,and
R3137 T3797 T3798 arg1Of utilized,in
R3138 T3800 T3798 arg2Of study,in
R3139 T3800 T3799 arg1Of study,this
R3140 T3802 T3801 arg2Of determined,were
R3141 T3802 T3803 arg1Of determined,in
R3142 T3806 T3803 arg2Of studies,in
R3143 T3806 T3804 arg1Of studies,previous
R3144 T3806 T3805 arg1Of studies,pilot
R3145 T3806 T3807 arg2Of studies,done
R3146 T3807 T3808 arg1Of done,in
R3147 T3810 T3808 arg2Of laboratory,in
R3148 T3810 T3809 arg1Of laboratory,our
R3149 T3810 T3811 arg1Of laboratory,","
R3150 T3810 T3812 arg1Of laboratory,which
R3151 T3810 T3813 arg1Of laboratory,examined
R3152 T3815 T3813 arg2Of effects,examined
R3153 T3815 T3814 arg1Of effects,the
R3154 T3815 T3816 arg1Of effects,of
R3155 T3815 T3821 arg1Of effects,on
R3156 T3818 T3816 arg2Of concentrations,of
R3157 T3818 T3817 arg1Of concentrations,increasing
R3158 T3818 T3819 arg1Of concentrations,of
R3159 T3820 T3819 arg2Of PTX,of
R3160 T3824 T3821 arg2Of production,on
R3161 T3824 T3822 arg1Of production,LPS-induced
R3162 T3824 T3823 arg1Of production,TNF-α
R3163 T3825 T3826 arg1Of LPS,at
R3164 T3825 T3834 arg1Of LPS,at
R3165 T3825 T3840 arg1Of LPS,were
R3166 T3828 T3826 arg2Of concentration,at
R3167 T3828 T3827 arg1Of concentration,a
R3168 T3828 T3829 arg1Of concentration,of
R3169 T3831 T3832 arg1Of μg/mL,and
R3170 T3832 T3829 arg2Of and,of
R3171 T3832 T3830 arg1Of and,1
R3172 T3833 T3832 arg2Of PTX,and
R3173 T3836 T3834 arg2Of concentration,at
R3174 T3836 T3835 arg1Of concentration,a
R3175 T3836 T3837 arg1Of concentration,of
R3176 T3839 T3837 arg2Of mM,of
R3177 T3839 T3838 arg1Of mM,20
R3178 T3843 T3840 arg2Of concentrations,were
R3179 T3843 T3841 arg1Of concentrations,the
R3180 T3843 T3842 arg1Of concentrations,minimum
R3181 T3843 T3844 arg1Of concentrations,necessary
R3182 T3846 T3844 arg2Of produce,necessary
R3183 T3846 T3845 arg1Of produce,to
R3184 T3846 T3857 arg1Of produce,","
R3185 T3846 T3858 arg1Of produce,respectively
R3186 T3846 T3859 arg1Of produce,","
R3187 T3846 T3860 arg1Of produce,in
R3188 T3848 T3849 arg1Of reproducible,and
R3189 T3850 T3849 arg2Of reliable,and
R3190 T3851 T3852 arg1Of up-regulation,and
R3191 T3852 T3846 arg2Of and,produce
R3192 T3852 T3847 arg1Of and,a
R3193 T3852 T3848 arg1Of and,reproducible
R3194 T3852 T3850 arg1Of and,reliable
R3195 T3852 T3854 arg1Of and,in
R3196 T3853 T3852 arg2Of down-regulation,and
R3197 T3856 T3854 arg2Of production,in
R3198 T3856 T3855 arg1Of production,TNF-α
R3199 T3862 T3860 arg2Of assays,in
R3200 T3862 T3861 arg1Of assays,quantitative
R3201 T3862 T3863 arg1Of assays,(
R3202 T3864 T3863 arg2Of data,(
R3203 T3864 T3866 arg2Of data,shown
R3204 T3866 T3865 arg1Of shown,not
R3205 T3867 T3863 arg3Of ),(
R3302 T4023 T4024 arg1Of Effect,of
R3303 T4023 T4026 arg1Of Effect,on
R3304 T4023 T4030 arg1Of Effect,by
R3305 T4025 T4024 arg2Of PTX,of
R3306 T4029 T4026 arg2Of production,on
R3307 T4029 T4027 arg1Of production,LPS-induced
R3308 T4029 T4028 arg1Of production,TNF-α
R3309 T4032 T4030 arg2Of cells,by
R3310 T4032 T4031 arg1Of cells,mononuclear
R3311 T4034 T4033 arg1Of concentration,The
R3312 T4034 T4035 arg1Of concentration,of
R3313 T4034 T4037 arg1Of concentration,was
R3314 T4034 T4039 arg2Of concentration,determined
R3315 T4036 T4035 arg2Of TNF-α,of
R3316 T4039 T4037 arg2Of determined,was
R3317 T4039 T4038 arg1Of determined,quantitatively
R3318 T4041 T4039 arg1Of ELISA,determined
R3319 T4041 T4040 arg2Of ELISA,by
R3320 T4041 T4042 arg1Of ELISA,in
R3321 T4044 T4042 arg2Of media,in
R3322 T4044 T4043 arg1Of media,the
R3323 T4044 T4045 arg1Of media,of
R3324 T4044 T4063 arg1Of media,","
R3325 T4044 T4064 arg1Of media,at
R3326 T4046 T4045 arg2Of cells,of
R3327 T4046 T4047 arg2Of cells,exposed
R3328 T4047 T4048 arg1Of exposed,to
R3329 T4050 T4048 arg2Of treatments,to
R3330 T4050 T4049 arg1Of treatments,the
R3331 T4050 T4051 arg2Of treatments,described
R3332 T4051 T4052 arg1Of described,above
R3333 T4051 T4053 arg1Of described,after
R3334 T4051 T4055 arg1Of described,for
R3335 T4054 T4053 arg2Of stimulation,after
R3336 T4056 T4055 arg2Of up,for
R3337 T4056 T4057 arg1Of up,to
R3338 T4059 T4057 arg2Of maximum,to
R3339 T4059 T4058 arg1Of maximum,a
R3340 T4059 T4060 arg1Of maximum,of
R3341 T4062 T4060 arg2Of hours,of
R3342 T4062 T4061 arg1Of hours,18
R3343 T4066 T4064 arg2Of intervals,at
R3344 T4066 T4065 arg1Of intervals,3-hour
R3345 T4068 T4067 arg1Of results,The
R3346 T4068 T4069 arg1Of results,are
R3347 T4068 T4070 arg2Of results,expressed
R3348 T4070 T4069 arg2Of expressed,are
R3349 T4070 T4071 arg1Of expressed,in
R3350 T4072 T4071 arg2Of pg/mL,in
R3696 T4475 T4476 arg1Of SDS-PAGE,and
R3697 T4476 T4478 arg1Of and,(
R3698 T4477 T4476 arg2Of Immunoblotting,and
R3699 T4479 T4480 arg1Of I-κBα,","
R3700 T4480 T4484 arg1Of ",",and
R3701 T4482 T4480 arg2Of p65,","
R3702 T4482 T4481 arg1Of p65,NF-κB
R3703 T4484 T4478 arg2Of and,(
R3704 T4484 T4483 arg1Of and,","
R3705 T4485 T4484 arg2Of CREB,and
R3706 T4486 T4478 arg3Of ),(
R3707 T4488 T4487 arg2Of stimulation,Following
R3708 T4490 T4491 arg1Of PBMCs,were
R3709 T4490 T4492 arg2Of PBMCs,washed
R3710 T4490 T4497 arg2Of PBMCs,centrifuged
R3711 T4490 T4499 arg1Of PBMCs,collect
R3712 T4492 T4493 arg1Of washed,with
R3713 T4492 T4496 arg1Of washed,and
R3714 T4495 T4493 arg2Of PBS,with
R3715 T4495 T4494 arg1Of PBS,ice-cold
R3716 T4496 T4487 arg1Of and,Following
R3717 T4496 T4489 arg1Of and,","
R3718 T4496 T4491 arg2Of and,were
R3719 T4497 T4496 arg2Of centrifuged,and
R3720 T4499 T4497 arg3Of collect,centrifuged
R3721 T4499 T4498 arg1Of collect,to
R3722 T4502 T4499 arg2Of pellet,collect
R3723 T4502 T4500 arg1Of pellet,a
R3724 T4502 T4501 arg1Of pellet,cell
R3725 T4504 T4503 arg1Of pellet,The
R3726 T4504 T4505 arg1Of pellet,was
R3727 T4504 T4506 arg2Of pellet,resuspended
R3728 T4506 T4505 arg2Of resuspended,was
R3729 T4506 T4507 arg1Of resuspended,in
R3730 T4511 T4507 arg2Of buffer,in
R3731 T4511 T4508 arg1Of buffer,ice-cold
R3732 T4511 T4509 arg1Of buffer,SDS
R3733 T4511 T4510 arg1Of buffer,sample
R3734 T4511 T4512 arg2Of buffer,supplemented
R3735 T4512 T4513 arg1Of supplemented,with
R3736 T4514 T4515 arg1Of 100,mM
R3737 T4516 T4513 arg2Of dithiothreitol,with
R3738 T4516 T4514 arg1Of dithiothreitol,100
R3739 T4517 T4518 arg1Of Cytoplasmic,and
R3740 T4519 T4518 arg2Of nuclear,and
R3741 T4520 T4517 arg1Of extracts,Cytoplasmic
R3742 T4520 T4519 arg1Of extracts,nuclear
R3743 T4520 T4521 arg1Of extracts,were
R3744 T4520 T4522 arg2Of extracts,isolated
R3745 T4522 T4521 arg2Of isolated,were
R3746 T4522 T4523 arg1Of isolated,with
R3747 T4522 T4536 arg1Of isolated,according
R3748 T4525 T4526 arg1Of nuclear,and
R3749 T4527 T4526 arg2Of cytoplasmic,and
R3750 T4529 T4523 arg2Of reagents,with
R3751 T4529 T4524 arg1Of reagents,NE-PER
R3752 T4529 T4525 arg1Of reagents,nuclear
R3753 T4529 T4527 arg1Of reagents,cytoplasmic
R3754 T4529 T4528 arg1Of reagents,extraction
R3755 T4529 T4530 arg1Of reagents,with
R3756 T4535 T4530 arg2Of Cocktail,with
R3757 T4535 T4531 arg1Of Cocktail,1x
R3758 T4535 T4532 arg1Of Cocktail,Halt
R3759 T4535 T4533 arg1Of Cocktail,Protease
R3760 T4535 T4534 arg1Of Cocktail,Inhibitor
R3761 T4537 T4536 arg2Of to,according
R3762 T4540 T4537 arg2Of instructions,to
R3763 T4540 T4538 arg1Of instructions,the
R3764 T4540 T4539 arg1Of instructions,manufacturer’s
R3765 T4542 T4541 arg1Of concentrations,Protein
R3766 T4542 T4543 arg1Of concentrations,were
R3767 T4542 T4544 arg2Of concentrations,determined
R3768 T4544 T4543 arg2Of determined,were
R3769 T4544 T4545 arg1Of determined,with
R3770 T4544 T4554 arg1Of determined,according
R3771 T4550 T4545 arg2Of kit,with
R3772 T4550 T4546 arg1Of kit,the
R3773 T4550 T4547 arg1Of kit,BCA
R3774 T4550 T4548 arg1Of kit,protein
R3775 T4550 T4549 arg1Of kit,reagent
R3776 T4550 T4551 arg1Of kit,for
R3777 T4553 T4551 arg2Of sample,for
R3778 T4553 T4552 arg1Of sample,each
R3779 T4555 T4554 arg2Of to,according
R3780 T4558 T4555 arg2Of curve,to
R3781 T4558 T4556 arg1Of curve,a
R3782 T4558 T4557 arg1Of curve,standardized
R3783 T4558 T4559 arg1Of curve,for
R3784 T4560 T4559 arg2Of albumin,for
R3785 T4562 T4561 arg1Of lysates,Cell
R3786 T4562 T4563 arg1Of lysates,were
R3787 T4562 T4564 arg2Of lysates,collected
R3788 T4564 T4563 arg2Of collected,were
R3789 T4564 T4565 arg1Of collected,by
R3790 T4566 T4565 arg2Of boiling,by
R3791 T4566 T4569 arg1Of boiling,in
R3792 T4568 T4566 arg2Of samples,boiling
R3793 T4568 T4567 arg1Of samples,the
R3794 T4573 T4569 arg2Of bath,in
R3795 T4573 T4570 arg1Of bath,a
R3796 T4573 T4571 arg1Of bath,100ºC
R3797 T4573 T4572 arg1Of bath,water
R3798 T4573 T4574 arg1Of bath,for
R3799 T4576 T4574 arg2Of minutes,for
R3800 T4576 T4575 arg1Of minutes,5
R3801 T4578 T4577 arg1Of μg,Ten
R3802 T4578 T4579 arg1Of μg,of
R3803 T4578 T4581 arg1Of μg,per
R3804 T4578 T4583 arg1Of μg,was
R3805 T4578 T4584 arg2Of μg,separated
R3806 T4578 T4597 arg2Of μg,transferred
R3807 T4580 T4579 arg2Of protein,of
R3808 T4582 T4581 arg2Of sample,per
R3809 T4584 T4589 arg1Of separated,through
R3810 T4584 T4596 arg1Of separated,and
R3811 T4588 T4584 arg1Of electrophoresis,separated
R3812 T4588 T4585 arg2Of electrophoresis,by
R3813 T4588 T4586 arg1Of electrophoresis,SDS-polyacrylamide
R3814 T4588 T4587 arg1Of electrophoresis,gel
R3815 T4590 T4591 arg1Of 8–16,%
R3816 T4595 T4589 arg2Of gels,through
R3817 T4595 T4590 arg1Of gels,8–16
R3818 T4595 T4592 arg1Of gels,tris-glycine
R3819 T4595 T4593 arg1Of gels,polyacrylamide
R3820 T4595 T4594 arg1Of gels,gradient
R3821 T4596 T4583 arg2Of and,was
R3822 T4597 T4596 arg2Of transferred,and
R3823 T4597 T4598 modOf transferred,to
R3824 T4599 T4598 arg1Of nitrocellulose,to
R3825 T4600 T4599 arg2Of membranes,nitrocellulose
R3826 T4602 T4601 arg1Of membranes,The
R3827 T4602 T4603 arg1Of membranes,were
R3828 T4602 T4604 arg2Of membranes,blocked
R3829 T4604 T4603 arg2Of blocked,were
R3830 T4604 T4605 arg1Of blocked,with
R3831 T4606 T4607 arg1Of 5,%
R3832 T4608 T4605 arg2Of milk,with
R3833 T4608 T4606 arg1Of milk,5
R3834 T4608 T4609 arg1Of milk,in
R3835 T4611 T4609 arg2Of saline/Tween,in
R3836 T4611 T4610 arg1Of saline/Tween,Tris-buffered
R3837 T4611 T4612 arg1Of saline/Tween,20
R3838 T4611 T4613 arg1Of saline/Tween,(
R3839 T4611 T4621 arg1Of saline/Tween,for
R3840 T4615 T4613 arg2Of Scientific,(
R3841 T4615 T4614 arg1Of Scientific,Fischer
R3842 T4615 T4616 arg1Of Scientific,","
R3843 T4617 T4616 arg2Of Pittsburgh,","
R3844 T4617 T4618 arg1Of Pittsburgh,","
R3845 T4619 T4618 arg2Of PA,","
R3846 T4620 T4613 arg3Of ),(
R3847 T4623 T4621 arg2Of hour,for
R3848 T4623 T4622 arg1Of hour,1
R3849 T4625 T4624 arg1Of extracts,Cytoplasmic
R3850 T4625 T4626 arg1Of extracts,were
R3851 T4625 T4627 arg2Of extracts,incubated
R3852 T4627 T4626 arg2Of incubated,were
R3853 T4627 T4628 arg1Of incubated,with
R3854 T4627 T4637 arg1Of incubated,","
R3855 T4627 T4638 arg1Of incubated,while
R3856 T4631 T4628 arg2Of antibody,with
R3857 T4631 T4629 arg2Of antibody,phosphorylated
R3858 T4631 T4630 arg1Of antibody,I-κBα
R3859 T4631 T4632 arg1Of antibody,(
R3860 T4633 T4634 arg1Of 1,:
R3861 T4634 T4632 arg2Of :,(
R3862 T4635 T4634 arg2Of 200,:
R3863 T4636 T4632 arg3Of ),(
R3864 T4640 T4639 arg1Of extracts,nuclear
R3865 T4640 T4641 arg1Of extracts,were
R3866 T4640 T4642 arg2Of extracts,incubated
R3867 T4642 T4638 arg2Of incubated,while
R3868 T4642 T4641 arg2Of incubated,were
R3869 T4642 T4643 arg1Of incubated,with
R3870 T4642 T4664 arg1Of incubated,at
R3871 T4648 T4644 arg1Of antibody,either
R3872 T4648 T4645 arg2Of antibody,phosphorylated
R3873 T4648 T4646 arg1Of antibody,NF-κB
R3874 T4648 T4647 arg1Of antibody,p65
R3875 T4648 T4649 arg1Of antibody,(
R3876 T4648 T4654 arg1Of antibody,or
R3877 T4650 T4651 arg1Of 1,:
R3878 T4651 T4649 arg2Of :,(
R3879 T4652 T4651 arg2Of 500,:
R3880 T4653 T4649 arg3Of ),(
R3881 T4654 T4643 arg2Of or,with
R3882 T4657 T4654 arg2Of antibody,or
R3883 T4657 T4655 arg2Of antibody,phosphorylated
R3884 T4657 T4656 arg1Of antibody,CREB
R3885 T4657 T4658 arg1Of antibody,(
R3886 T4657 T4663 arg1Of antibody,overnight
R3887 T4659 T4660 arg1Of 1,:
R3888 T4660 T4658 arg2Of :,(
R3889 T4661 T4660 arg2Of 500,:
R3890 T4662 T4658 arg3Of ),(
R3891 T4665 T4664 arg2Of 4°C,at
R3892 T4665 T4666 arg1Of 4°C,in
R3893 T4668 T4666 arg2Of experiments,in
R3894 T4668 T4667 arg1Of experiments,separate
R3895 T4670 T4669 arg1Of membranes,The
R3896 T4670 T4671 arg1Of membranes,were
R3897 T4670 T4672 arg2Of membranes,washed
R3898 T4670 T4678 arg2Of membranes,incubated
R3899 T4672 T4673 arg1Of washed,with
R3900 T4672 T4677 arg1Of washed,and
R3901 T4675 T4673 arg2Of saline/Tween,with
R3902 T4675 T4674 arg1Of saline/Tween,Tris-buffered
R3903 T4675 T4676 arg1Of saline/Tween,20
R3904 T4677 T4671 arg2Of and,were
R3905 T4678 T4677 arg2Of incubated,and
R3906 T4678 T4679 arg1Of incubated,for
R3907 T4678 T4682 arg1Of incubated,at
R3908 T4681 T4679 arg2Of hour,for
R3909 T4681 T4680 arg1Of hour,1
R3910 T4684 T4682 arg2Of temperature,at
R3911 T4684 T4683 arg1Of temperature,room
R3912 T4684 T4685 arg1Of temperature,with
R3913 T4688 T4685 arg2Of antibody,with
R3914 T4688 T4686 arg1Of antibody,the
R3915 T4688 T4687 arg1Of antibody,secondary
R3916 T4693 T4690 arg1Of IgG,horseradish
R3917 T4693 T4691 arg1Of IgG,peroxidase-linked
R3918 T4693 T4692 arg1Of IgG,anti-rabbit
R3919 T4693 T4694 arg1Of IgG,diluted
R3920 T4694 T4671 modOf diluted,were
R3921 T4694 T4689 arg1Of diluted,","
R3922 T4694 T4696 arg1Of diluted,:
R3923 T4695 T4694 arg2Of 1,diluted
R3924 T4697 T4694 arg3Of 2000,diluted
R3925 T4697 T4698 arg1Of 2000,in
R3926 T4699 T4698 arg2Of blocking,in
R3927 T4700 T4699 arg2Of solution,blocking
R3928 T4703 T4701 arg2Of washing,After
R3929 T4703 T4702 arg2Of washing,repeated
R3930 T4703 T4704 arg1Of washing,of
R3931 T4706 T4704 arg2Of membrane,of
R3932 T4706 T4705 arg1Of membrane,the
R3933 T4713 T4708 arg1Of Kit,the
R3934 T4713 T4709 arg1Of Kit,Supersignal
R3935 T4713 T4710 arg1Of Kit,West
R3936 T4713 T4711 arg1Of Kit,Pico
R3937 T4713 T4712 arg1Of Kit,Chemiluminescent
R3938 T4713 T4714 arg1Of Kit,was
R3939 T4713 T4715 arg2Of Kit,applied
R3940 T4715 T4701 arg1Of applied,After
R3941 T4715 T4707 arg1Of applied,","
R3942 T4715 T4714 arg2Of applied,was
R3943 T4715 T4716 arg1Of applied,for
R3944 T4718 T4716 arg2Of detection,for
R3945 T4718 T4717 arg1Of detection,antibody
R3946 T4718 T4719 arg1Of detection,per
R3947 T4722 T4719 arg2Of instructions,per
R3948 T4722 T4720 arg1Of instructions,the
R3949 T4722 T4721 arg1Of instructions,manufacturer’s
R3950 T4725 T4723 arg1Of data,Western
R3951 T4725 T4724 arg1Of data,blot
R3952 T4725 T4726 arg1Of data,is
R3953 T4725 T4727 arg2Of data,presented
R3954 T4727 T4726 arg2Of presented,is
R3955 T4727 T4728 arg1Of presented,as
R3956 T4730 T4728 arg2Of percentage,as
R3957 T4730 T4729 arg1Of percentage,a
R3958 T4730 T4731 arg1Of percentage,of
R3959 T4733 T4731 arg2Of stimulation,of
R3960 T4733 T4732 arg1Of stimulation,LPS
R3961 T4735 T4734 arg1Of percentage,The
R3962 T4735 T4736 arg1Of percentage,of
R3963 T4735 T4739 arg1Of percentage,was
R3964 T4735 T4740 arg2Of percentage,calculated
R3965 T4738 T4736 arg2Of stimulation,of
R3966 T4738 T4737 arg1Of stimulation,LPS
R3967 T4740 T4739 arg2Of calculated,was
R3968 T4740 T4741 arg1Of calculated,by
R3969 T4742 T4741 arg2Of dividing,by
R3970 T4747 T4742 arg2Of total,dividing
R3971 T4747 T4743 arg1Of total,the
R3972 T4747 T4744 arg1Of total,mean
R3973 T4747 T4745 arg1Of total,band
R3974 T4747 T4746 arg1Of total,pixel
R3975 T4747 T4748 arg1Of total,for
R3976 T4751 T4748 arg2Of arm,for
R3977 T4751 T4749 arg1Of arm,each
R3978 T4751 T4750 arg1Of arm,treatment
R3979 T4751 T4752 arg2Of arm,divided
R3980 T4757 T4752 arg1Of total,divided
R3981 T4757 T4753 arg2Of total,by
R3982 T4757 T4754 arg1Of total,the
R3983 T4757 T4755 arg1Of total,mean
R3984 T4757 T4756 arg1Of total,pixel
R3985 T4757 T4758 arg1Of total,of
R3986 T4759 T4758 arg2Of samples,of
R3987 T4759 T4760 arg2Of samples,stimulated
R3988 T4759 T4764 arg1Of samples,multiplying
R3989 T4760 T4761 arg1Of stimulated,with
R3990 T4760 T4763 arg1Of stimulated,and
R3991 T4762 T4761 arg2Of LPS,with
R3992 T4764 T4763 arg2Of multiplying,and
R3993 T4764 T4765 arg1Of multiplying,by
R3994 T4766 T4765 arg2Of 100,by
R3995 T4770 T4769 arg1Of stimulation,LPS
R3996 T4770 T4771 arg1Of stimulation,is
R3997 T4770 T4772 arg2Of stimulation,reported
R3998 T4772 T4771 arg2Of reported,is
R3999 T4772 T4773 arg1Of reported,as
R4000 T4772 T4776 arg1Of reported,and
R4001 T4775 T4773 arg2Of %,as
R4002 T4775 T4774 arg1Of %,100
R4003 T4776 T4767 arg1Of and,Thus
R4004 T4776 T4768 arg1Of and,","
R4005 T4777 T4778 arg1Of each,of
R4006 T4777 T4782 arg1Of each,is
R4007 T4777 T4783 arg2Of each,reported
R4008 T4781 T4778 arg2Of arms,of
R4009 T4781 T4779 arg1Of arms,the
R4010 T4781 T4780 arg1Of arms,treatment
R4011 T4783 T4776 arg2Of reported,and
R4012 T4783 T4782 arg2Of reported,is
R4013 T4783 T4784 arg1Of reported,as
R4014 T4786 T4784 arg2Of percent,as
R4015 T4786 T4785 arg1Of percent,a
R4016 T4786 T4787 arg1Of percent,of
R4017 T4789 T4787 arg2Of stimulation,of
R4018 T4789 T4788 arg1Of stimulation,LPS
R4358 T5231 T5232 arg1Of NF-κB,and
R4359 T5233 T5232 arg2Of CREB,and
R4360 T5237 T5231 arg1Of Assay,NF-κB
R4361 T5237 T5233 arg1Of Assay,CREB
R4362 T5237 T5234 arg1Of Assay,Electrophoretic
R4363 T5237 T5235 arg1Of Assay,Mobility
R4364 T5237 T5236 arg1Of Assay,Shift
R4365 T5237 T5238 arg1Of Assay,(
R4366 T5239 T5238 arg2Of EMSA,(
R4367 T5240 T5238 arg3Of ),(
R4368 T5245 T5243 arg1Of EMSA,LightShift
R4369 T5245 T5244 arg1Of EMSA,Chemiluminescent
R4370 T5246 T5241 arg1Of Kit,The
R4371 T5246 T5242 arg1Of Kit,non-radioactive
R4372 T5246 T5245 arg1Of Kit,EMSA
R4373 T5246 T5247 arg1Of Kit,was
R4374 T5246 T5248 arg2Of Kit,used
R4375 T5248 T5247 arg2Of used,was
R4376 T5248 T5249 modOf used,to
R4377 T5250 T5249 arg1Of detect,to
R4378 T5252 T5250 arg2Of interactions,detect
R4379 T5252 T5251 arg1Of interactions,DNA-protein
R4380 T5259 T5253 arg1Of oligonucleotide,The
R4381 T5259 T5254 arg1Of oligonucleotide,NF-κB
R4382 T5259 T5255 arg1Of oligonucleotide,3′
R4383 T5259 T5256 arg1Of oligonucleotide,biotin
R4384 T5259 T5257 arg1Of oligonucleotide,end-labeled
R4385 T5259 T5258 arg1Of oligonucleotide,DNA
R4386 T5259 T5260 arg2Of oligonucleotide,used
R4387 T5259 T5267 arg1Of oligonucleotide,was
R4388 T5260 T5261 arg1Of used,as
R4389 T5263 T5261 arg2Of probe,as
R4390 T5263 T5262 arg1Of probe,a
R4391 T5263 T5264 arg1Of probe,for
R4392 T5266 T5264 arg2Of EMSA,for
R4393 T5266 T5265 arg1Of EMSA,the
R4394 T5272 T5267 arg2Of construct,was
R4395 T5272 T5268 arg1Of construct,a
R4396 T5272 T5269 arg1Of construct,42-bp
R4397 T5272 T5270 arg1Of construct,double
R4398 T5272 T5271 arg1Of construct,stranded
R4399 T5272 T5273 arg1Of construct,(
R4400 T5272 T5278 arg1Of construct,containing
R4401 T5276 T5273 arg2Of 3′,(
R4402 T5276 T5274 arg1Of 3′,5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4403 T5276 T5275 arg1Of 3′,–
R4404 T5277 T5273 arg3Of ),(
R4405 T5284 T5278 arg2Of sites,containing
R4406 T5284 T5279 arg1Of sites,two
R4407 T5284 T5280 arg1Of sites,tandemly
R4408 T5284 T5281 arg2Of sites,repeated
R4409 T5284 T5282 arg1Of sites,NF-κB
R4410 T5284 T5283 arg1Of sites,binding
R4411 T5284 T5285 arg1Of sites,(
R4412 T5286 T5285 arg2Of underlined,(
R4413 T5287 T5285 arg3Of ),(
R4414 T5288 T5289 arg1Of Specificity,was
R4415 T5288 T5290 arg2Of Specificity,determined
R4416 T5290 T5289 arg2Of determined,was
R4417 T5294 T5290 arg1Of assay,determined
R4418 T5294 T5291 arg2Of assay,by
R4419 T5294 T5292 arg1Of assay,a
R4420 T5294 T5293 arg1Of assay,competition
R4421 T5294 T5295 arg1Of assay,with
R4422 T5297 T5295 arg2Of addition,with
R4423 T5297 T5296 arg1Of addition,the
R4424 T5297 T5298 arg1Of addition,of
R4425 T5300 T5299 arg1Of molar,200
R4426 T5301 T5298 arg2Of excess,of
R4427 T5301 T5300 arg1Of excess,molar
R4428 T5301 T5302 arg1Of excess,of
R4429 T5305 T5303 arg1Of stranded,unlabeled
R4430 T5305 T5304 arg1Of stranded,double
R4431 T5307 T5302 arg2Of oligonucleotide,of
R4432 T5307 T5305 arg1Of oligonucleotide,stranded
R4433 T5307 T5306 arg1Of oligonucleotide,NF-κB
R4434 T5314 T5308 arg1Of oligonucleotide,The
R4435 T5314 T5309 arg1Of oligonucleotide,CREB
R4436 T5314 T5310 arg1Of oligonucleotide,3′
R4437 T5314 T5311 arg1Of oligonucleotide,biotin
R4438 T5314 T5312 arg1Of oligonucleotide,end-labeled
R4439 T5314 T5313 arg1Of oligonucleotide,DNA
R4440 T5314 T5315 arg2Of oligonucleotide,used
R4441 T5314 T5322 arg1Of oligonucleotide,was
R4442 T5315 T5316 arg1Of used,as
R4443 T5318 T5316 arg2Of probe,as
R4444 T5318 T5317 arg1Of probe,a
R4445 T5318 T5319 arg1Of probe,for
R4446 T5321 T5319 arg2Of EMSA,for
R4447 T5321 T5320 arg1Of EMSA,the
R4448 T5327 T5322 arg2Of construct,was
R4449 T5327 T5323 arg1Of construct,a
R4450 T5327 T5324 arg1Of construct,23-bp
R4451 T5327 T5325 arg1Of construct,double
R4452 T5327 T5326 arg1Of construct,stranded
R4453 T5327 T5328 arg1Of construct,(
R4454 T5327 T5336 arg1Of construct,containing
R4455 T5334 T5328 arg2Of 3′,(
R4456 T5334 T5329 arg1Of 3′,5′-TTT
R4457 T5334 T5330 arg1Of 3′,TCG
R4458 T5334 T5331 arg1Of 3′,AGC
R4459 T5334 T5332 arg1Of 3′,TCTGACGTCAGA-GC
R4460 T5334 T5333 arg1Of 3′,–
R4461 T5335 T5328 arg3Of ),(
R4462 T5340 T5336 arg2Of sequence,containing
R4463 T5340 T5337 arg1Of sequence,the
R4464 T5340 T5338 arg1Of sequence,CRE
R4465 T5340 T5339 arg1Of sequence,consensus
R4466 T5340 T5341 arg1Of sequence,(
R4467 T5342 T5341 arg2Of underlined,(
R4468 T5343 T5341 arg3Of ),(
R4469 T5344 T5345 arg1Of Specificity,was
R4470 T5344 T5346 arg2Of Specificity,determined
R4471 T5346 T5345 arg2Of determined,was
R4472 T5350 T5346 arg1Of assay,determined
R4473 T5350 T5347 arg2Of assay,by
R4474 T5350 T5348 arg1Of assay,a
R4475 T5350 T5349 arg1Of assay,competition
R4476 T5350 T5351 arg1Of assay,with
R4477 T5353 T5351 arg2Of addition,with
R4478 T5353 T5352 arg1Of addition,the
R4479 T5353 T5354 arg1Of addition,of
R4480 T5356 T5355 arg1Of molar,200
R4481 T5357 T5354 arg2Of excess,of
R4482 T5357 T5356 arg1Of excess,molar
R4483 T5357 T5358 arg1Of excess,of
R4484 T5361 T5359 arg1Of stranded,unlabeled
R4485 T5361 T5360 arg1Of stranded,double
R4486 T5363 T5358 arg2Of oligonucleotide,of
R4487 T5363 T5361 arg1Of oligonucleotide,stranded
R4488 T5363 T5362 arg1Of oligonucleotide,CREB
R4489 T5365 T5364 arg1Of extracts,Nuclear
R4490 T5365 T5366 arg1Of extracts,(
R4491 T5365 T5370 arg1Of extracts,were
R4492 T5365 T5371 arg2Of extracts,incubated
R4493 T5368 T5366 arg2Of μg,(
R4494 T5368 T5367 arg1Of μg,10
R4495 T5369 T5366 arg3Of ),(
R4496 T5371 T5370 arg2Of incubated,were
R4497 T5371 T5372 arg1Of incubated,with
R4498 T5373 T5374 arg1Of 5,nM
R4499 T5375 T5373 arg1Of NF-κB,5
R4500 T5375 T5376 arg1Of NF-κB,or
R4501 T5376 T5372 arg2Of or,with
R4502 T5378 T5376 arg2Of probe,or
R4503 T5378 T5377 arg1Of probe,CREB
R4504 T5378 T5379 arg1Of probe,(
R4505 T5380 T5379 arg2Of NF-kB,(
R4506 T5381 T5379 arg3Of :,(
R4507 T5384 T5382 arg1Of buffer,1x
R4508 T5384 T5383 arg1Of buffer,binding
R4509 T5384 T5385 arg1Of buffer,","
R4510 T5385 T5389 arg1Of ",",","
R4511 T5386 T5387 arg1Of 50,mM
R4512 T5388 T5385 arg2Of KCl,","
R4513 T5388 T5386 arg1Of KCl,50
R4514 T5389 T5393 arg1Of ",",","
R4515 T5390 T5391 arg1Of 1,mM
R4516 T5392 T5389 arg2Of EDTA,","
R4517 T5392 T5390 arg1Of EDTA,1
R4518 T5393 T5397 arg1Of ",",","
R4519 T5394 T5395 arg1Of 1,mM
R4520 T5396 T5393 arg2Of DTT,","
R4521 T5396 T5394 arg1Of DTT,1
R4522 T5397 T5401 arg1Of ",",","
R4523 T5398 T5399 arg1Of 0.1,%
R4524 T5400 T5397 arg2Of NP40,","
R4525 T5400 T5398 arg1Of NP40,0.1
R4526 T5401 T5406 arg1Of ",",and
R4527 T5403 T5402 arg1Of %,10
R4528 T5404 T5401 arg2Of glycerol,","
R4529 T5404 T5403 arg1Of glycerol,%
R4530 T5406 T5405 arg1Of and,","
R4531 T5410 T5406 arg2Of dI-dC,and
R4532 T5410 T5407 arg1Of dI-dC,50
R4533 T5410 T5408 arg1Of dI-dC,ng/μl
R4534 T5410 T5409 arg1Of dI-dC,poly
R4535 T5410 T5411 arg1Of dI-dC,","
R4536 T5412 T5411 arg2Of CREB,","
R4537 T5412 T5413 arg1Of CREB,:
R4538 T5416 T5414 arg1Of buffer,1x
R4539 T5416 T5415 arg1Of buffer,binding
R4540 T5416 T5417 arg1Of buffer,","
R4541 T5418 T5419 arg1Of 20,mM
R4542 T5420 T5417 arg2Of Tris,","
R4543 T5420 T5418 arg1Of Tris,20
R4544 T5420 T5421 arg1Of Tris,","
R4545 T5422 T5421 arg2Of pH,","
R4546 T5422 T5423 arg1Of pH,7.5
R4547 T5425 T5426 arg1Of 50,mM
R4548 T5427 T5425 arg1Of KCl,50
R4549 T5427 T5428 arg1Of KCl,","
R4550 T5428 T5432 arg1Of ",",","
R4551 T5429 T5430 arg1Of 1,mM
R4552 T5431 T5428 arg2Of EDTA,","
R4553 T5431 T5429 arg1Of EDTA,1
R4554 T5433 T5434 arg1Of 1,mM
R4555 T5435 T5432 arg2Of DTT,","
R4556 T5435 T5433 arg1Of DTT,1
R4557 T5437 T5438 arg1Of 0.10,%
R4558 T5439 T5437 arg1Of NP40,0.10
R4559 T5439 T5440 arg1Of NP40,","
R4560 T5440 T5444 arg1Of ",",","
R4561 T5441 T5442 arg1Of 6,%
R4562 T5443 T5440 arg2Of glycerol,","
R4563 T5443 T5441 arg1Of glycerol,6
R4564 T5444 T5448 arg1Of ",",and
R4565 T5445 T5446 arg1Of 0.1,mg/mL
R4566 T5447 T5444 arg2Of BSA,","
R4567 T5447 T5445 arg1Of BSA,0.1
R4568 T5452 T5448 arg2Of dI-dC,and
R4569 T5452 T5449 arg1Of dI-dC,50
R4570 T5452 T5450 arg1Of dI-dC,ng/μl
R4571 T5452 T5451 arg1Of dI-dC,poly
R4572 T5457 T5454 arg2Of electrophoresed,and
R4573 T5457 T5455 arg2Of electrophoresed,were
R4574 T5457 T5456 arg1Of electrophoresed,then
R4575 T5457 T5458 arg1Of electrophoresed,through
R4576 T5460 T5461 arg1Of 6,%
R4577 T5464 T5458 arg2Of gel,through
R4578 T5464 T5459 arg1Of gel,a
R4579 T5464 T5460 arg1Of gel,6
R4580 T5464 T5462 arg1Of gel,DNA
R4581 T5464 T5463 arg1Of gel,retardation
R4582 T5464 T5465 arg1Of gel,at
R4583 T5466 T5465 arg2Of 100V,at
R4584 T5466 T5467 arg1Of 100V,for
R4585 T5469 T5467 arg2Of minutes,for
R4586 T5469 T5468 arg1Of minutes,90
R4587 T5471 T5470 arg1Of gels,The
R4588 T5471 T5472 arg1Of gels,were
R4589 T5471 T5474 arg2Of gels,transferred
R4590 T5474 T5472 arg2Of transferred,were
R4591 T5474 T5473 arg1Of transferred,electrophoretically
R4592 T5474 T5475 arg1Of transferred,at
R4593 T5476 T5475 arg2Of 380mA,at
R4594 T5476 T5477 arg1Of 380mA,for
R4595 T5476 T5489 arg1Of 380mA,immediately
R4596 T5477 T5488 arg1Of for,and
R4597 T5479 T5477 arg2Of hour,for
R4598 T5479 T5478 arg1Of hour,1
R4599 T5479 T5480 arg1Of hour,on
R4600 T5479 T5482 arg1Of hour,to
R4601 T5481 T5480 arg2Of ice,on
R4602 T5485 T5484 arg1Of charged,positively
R4603 T5487 T5482 arg2Of membrane,to
R4604 T5487 T5483 arg1Of membrane,a
R4605 T5487 T5485 arg1Of membrane,charged
R4606 T5487 T5486 arg1Of membrane,nylon
R4607 T5489 T5488 arg2Of immediately,and
R4608 T5489 T5490 arg1Of immediately,cross-linked
R4609 T5490 T5491 arg1Of cross-linked,for
R4610 T5493 T5491 arg2Of minutes,for
R4611 T5493 T5492 arg1Of minutes,15
R4612 T5493 T5494 arg1Of minutes,with
R4613 T5497 T5494 arg2Of transilluminator,with
R4614 T5497 T5495 arg1Of transilluminator,a
R4615 T5497 T5496 arg1Of transilluminator,UV
R4616 T5497 T5498 arg2Of transilluminator,equipped
R4617 T5498 T5499 arg1Of equipped,with
R4618 T5501 T5502 arg1Of 312,nm
R4619 T5503 T5499 arg2Of bulb,with
R4620 T5503 T5500 arg1Of bulb,a
R4621 T5503 T5501 arg1Of bulb,312
R4622 T5506 T5504 arg1Of conjugate,Streptavidin-horseradish
R4623 T5506 T5505 arg1Of conjugate,peroxidase
R4624 T5506 T5507 arg1Of conjugate,and
R4625 T5507 T5512 arg1Of and,were
R4626 T5507 T5513 arg2Of and,used
R4627 T5511 T5507 arg2Of Substrate,and
R4628 T5511 T5508 arg1Of Substrate,the
R4629 T5511 T5509 arg1Of Substrate,LightShift
R4630 T5511 T5510 arg1Of Substrate,Chemiluminescent
R4631 T5513 T5512 arg2Of used,were
R4632 T5515 T5513 arg3Of detect,used
R4633 T5515 T5514 arg1Of detect,to
R4634 T5519 T5515 arg2Of DNA,detect
R4635 T5519 T5516 arg1Of DNA,the
R4636 T5519 T5517 arg1Of DNA,biotin
R4637 T5519 T5518 arg1Of DNA,end-labeled
R4638 T5522 T5520 arg1Of membranes,The
R4639 T5522 T5521 arg1Of membranes,nylon
R4640 T5522 T5523 arg1Of membranes,were
R4641 T5522 T5524 arg2Of membranes,exposed
R4642 T5524 T5523 arg2Of exposed,were
R4643 T5524 T5525 arg1Of exposed,to
R4644 T5524 T5528 arg1Of exposed,for
R4645 T5527 T5525 arg2Of film,to
R4646 T5527 T5526 arg1Of film,x-ray
R4647 T5530 T5528 arg2Of minutes,for
R4648 T5530 T5529 arg1Of minutes,1–3
R4649 T5530 T5531 arg1Of minutes,for
R4650 T5532 T5531 arg2Of detection,for
R4831 T5799 T5800 arg1Of Role,of
R4832 T5799 T5802 arg1Of Role,on
R4833 T5801 T5800 arg2Of PKA,of
R4834 T5807 T5802 arg2Of production,on
R4835 T5807 T5803 arg1Of production,LPS-induced
R4836 T5807 T5804 arg1Of production,mononuclear
R4837 T5807 T5805 arg1Of production,cell
R4838 T5807 T5806 arg1Of production,TNF-α
R4839 T5809 T5808 arg1Of evaluate,To
R4840 T5809 T5842 arg1Of evaluate,was
R4841 T5809 T5843 arg2Of evaluate,utilized
R4842 T5811 T5810 arg1Of role,the
R4843 T5811 T5812 arg1Of role,of
R4844 T5811 T5814 arg1Of role,on
R4845 T5811 T5817 arg1Of role,in
R4846 T5811 T5820 arg1Of role,and
R4847 T5813 T5812 arg2Of PKA,of
R4848 T5816 T5814 arg2Of production,on
R4849 T5816 T5815 arg1Of production,TNF-α
R4850 T5819 T5817 arg2Of cells,in
R4851 T5819 T5818 arg1Of cells,mononuclear
R4852 T5820 T5809 arg2Of and,evaluate
R4853 T5822 T5820 arg2Of involvement,and
R4854 T5822 T5821 arg1Of involvement,its
R4855 T5822 T5823 arg1Of involvement,in
R4856 T5825 T5823 arg2Of attenuation,in
R4857 T5825 T5824 arg1Of attenuation,the
R4858 T5825 T5826 arg1Of attenuation,of
R4859 T5829 T5826 arg2Of production,of
R4860 T5829 T5827 arg1Of production,LPS-induced
R4861 T5829 T5828 arg1Of production,TNF-α
R4862 T5829 T5830 arg2Of production,observed
R4863 T5830 T5831 arg1Of observed,following
R4864 T5833 T5831 arg2Of treatment,following
R4865 T5833 T5832 arg1Of treatment,PTX
R4866 T5833 T5834 arg1Of treatment,","
R4867 T5833 T5836 arg1Of treatment,","
R4868 T5835 T5834 arg2Of H89,","
R4869 T5840 T5836 arg2Of inhibitor,","
R4870 T5840 T5837 arg1Of inhibitor,a
R4871 T5840 T5838 arg1Of inhibitor,specific
R4872 T5840 T5839 arg1Of inhibitor,PKA
R4873 T5843 T5841 arg1Of utilized,","
R4874 T5843 T5842 arg2Of utilized,was
R4875 T5846 T5844 arg2Of cells,Isolated
R4876 T5846 T5845 arg1Of cells,mononuclear
R4877 T5846 T5847 arg1Of cells,were
R4878 T5846 T5848 arg2Of cells,incubated
R4879 T5848 T5847 arg2Of incubated,were
R4880 T5848 T5849 arg1Of incubated,according
R4881 T5850 T5849 arg2Of to,according
R4882 T5853 T5850 arg2Of groups,to
R4883 T5853 T5851 arg1Of groups,the
R4884 T5853 T5852 arg1Of groups,treatment
R4885 T5853 T5854 arg2Of groups,described
R4886 T5854 T5855 arg1Of described,above
R4887 T5854 T5856 arg1Of described,in
R4888 T5858 T5859 arg1Of presence,and
R4889 T5859 T5856 arg2Of and,in
R4890 T5859 T5857 arg1Of and,the
R4891 T5859 T5861 arg1Of and,of
R4892 T5859 T5863 arg1Of and,with
R4893 T5860 T5859 arg2Of absence,and
R4894 T5862 T5861 arg2Of pretreatment,of
R4895 T5864 T5863 arg2Of H89,with
R4896 T5864 T5865 arg1Of H89,(
R4897 T5864 T5869 arg1Of H89,for
R4898 T5867 T5865 arg2Of μM,(
R4899 T5867 T5866 arg1Of μM,10
R4900 T5868 T5865 arg3Of ),(
R4901 T5871 T5869 arg2Of hour,for
R4902 T5871 T5870 arg1Of hour,1
R4903 T5871 T5872 arg1Of hour,at
R4904 T5873 T5872 arg2Of 37ºC,at
R4905 T5875 T5874 arg1Of dose,The
R4906 T5875 T5876 arg1Of dose,of
R4907 T5875 T5878 arg1Of dose,was
R4908 T5875 T5879 arg2Of dose,chosen
R4909 T5877 T5876 arg2Of H89,of
R4910 T5879 T5878 arg2Of chosen,was
R4911 T5879 T5880 modOf chosen,based
R4912 T5879 T5881 arg1Of chosen,on
R4913 T5883 T5881 arg2Of work,on
R4914 T5883 T5882 arg1Of work,previous
R4915 T5883 T5884 arg1Of work,which
R4916 T5883 T5885 arg1Of work,demonstrated
R4917 T5886 T5887 arg1Of specific,and
R4918 T5888 T5887 arg2Of complete,and
R4919 T5889 T5885 arg2Of inhibition,demonstrated
R4920 T5889 T5886 arg1Of inhibition,specific
R4921 T5889 T5888 arg1Of inhibition,complete
R4922 T5889 T5890 arg1Of inhibition,of
R4923 T5891 T5890 arg2Of PKA,of
R4924 T5894 T5892 arg2Of concentration.10,at
R4925 T5894 T5893 arg1Of concentration.10,this
R4926 T5894 T5895 arg1Of concentration.10,Since
R4927 T5897 T5896 arg1Of activity,the
R4928 T5897 T5898 arg1Of activity,of
R4929 T5897 T5901 arg1Of activity,is
R4930 T5897 T5903 arg1Of activity,specific
R4931 T5897 T5905 arg1Of activity,consistent
R4932 T5900 T5898 arg2Of inhibitor,of
R4933 T5900 T5899 arg1Of inhibitor,this
R4934 T5901 T5895 arg2Of is,Since
R4935 T5903 T5904 arg1Of specific,and
R4936 T5904 T5901 arg2Of and,is
R4937 T5904 T5902 arg1Of and,both
R4938 T5904 T5906 arg1Of and,at
R4939 T5905 T5904 arg2Of consistent,and
R4940 T5909 T5906 arg2Of dose,at
R4941 T5909 T5907 arg1Of dose,this
R4942 T5909 T5908 arg2Of dose,specified
R4943 T5911 T5912 arg1Of we,did
R4944 T5911 T5914 arg1Of we,include
R4945 T5914 T5885 arg3Of include,demonstrated
R4946 T5914 T5892 arg1Of include,at
R4947 T5914 T5910 arg1Of include,","
R4948 T5914 T5912 arg2Of include,did
R4949 T5914 T5913 arg1Of include,not
R4950 T5915 T5914 arg2Of experiments,include
R4951 T5915 T5916 arg1Of experiments,with
R4952 T5919 T5916 arg2Of inhibitors,with
R4953 T5919 T5917 arg1Of inhibitors,additional
R4954 T5919 T5918 arg1Of inhibitors,PKA
R4955 T5919 T5920 arg1Of inhibitors,with
R4956 T5922 T5920 arg2Of set,with
R4957 T5922 T5921 arg1Of set,this
R4958 T5922 T5923 arg1Of set,of
R4959 T5924 T5923 arg2Of experiments,of
R4960 T5927 T5925 arg1Of concentration,The
R4961 T5927 T5926 arg1Of concentration,TNF-α
R4962 T5927 T5928 arg1Of concentration,in
R4963 T5927 T5931 arg1Of concentration,was
R4964 T5927 T5932 arg2Of concentration,measured
R4965 T5930 T5928 arg2Of supernatant,in
R4966 T5930 T5929 arg1Of supernatant,the
R4967 T5932 T5931 arg2Of measured,was
R4968 T5932 T5933 arg1Of measured,quantitatively
R4969 T5935 T5932 arg1Of ELISA,measured
R4970 T5935 T5934 arg2Of ELISA,by
R4971 T5937 T5936 arg1Of results,The
R4972 T5937 T5938 arg1Of results,are
R4973 T5937 T5939 arg2Of results,expressed
R4974 T5939 T5938 arg2Of expressed,are
R4975 T5939 T5940 arg1Of expressed,in
R4976 T5941 T5940 arg2Of pg/mL,in
R5103 T6141 T6140 arg1Of Analysis,Statistical
R5104 T6144 T6142 arg1Of results,The
R5105 T6144 T6143 arg1Of results,experimental
R5106 T6144 T6145 arg2Of results,obtained
R5107 T6144 T6149 arg1Of results,were
R5108 T6144 T6150 arg2Of results,derived
R5109 T6145 T6146 arg1Of obtained,in
R5110 T6148 T6146 arg2Of study,in
R5111 T6148 T6147 arg1Of study,this
R5112 T6150 T6149 arg2Of derived,were
R5113 T6150 T6151 arg1Of derived,from
R5114 T6154 T6151 arg2Of experiments,from
R5115 T6154 T6152 arg1Of experiments,four
R5116 T6154 T6153 arg1Of experiments,separate
R5117 T6154 T6155 arg1Of experiments,with
R5118 T6158 T6155 arg2Of donors,with
R5119 T6158 T6156 arg1Of donors,healthy
R5120 T6158 T6157 arg1Of donors,volunteer
R5121 T6160 T6159 arg1Of assay,Each
R5122 T6160 T6161 arg1Of assay,was
R5123 T6160 T6162 arg2Of assay,performed
R5124 T6160 T6166 arg1Of assay,triplicate
R5125 T6162 T6161 arg2Of performed,was
R5126 T6162 T6163 arg1Of performed,in
R5127 T6162 T6165 arg1Of performed,or
R5128 T6164 T6163 arg2Of duplicate,in
R5129 T6166 T6165 arg2Of triplicate,or
R5130 T6166 T6167 arg1Of triplicate,where
R5131 T6168 T6167 arg2Of appropriate,where
R5132 T6169 T6170 arg1Of Data,is
R5133 T6169 T6171 arg2Of Data,presented
R5134 T6171 T6170 arg2Of presented,is
R5135 T6171 T6172 arg1Of presented,as
R5136 T6176 T6172 arg2Of SEM,as
R5137 T6176 T6173 arg1Of SEM,the
R5138 T6176 T6174 arg1Of SEM,mean
R5139 T6176 T6175 arg1Of SEM,±
R5140 T6178 T6177 arg1Of differences,Statistical
R5141 T6178 T6179 arg1Of differences,between
R5142 T6178 T6181 arg1Of differences,were
R5143 T6178 T6182 arg2Of differences,determined
R5144 T6180 T6179 arg2Of groups,between
R5145 T6182 T6181 arg2Of determined,were
R5146 T6185 T6182 arg1Of analysis,determined
R5147 T6185 T6183 arg2Of analysis,by
R5148 T6185 T6184 arg1Of analysis,one-way
R5149 T6185 T6186 arg1Of analysis,of
R5150 T6185 T6191 arg1Of analysis,with
R5151 T6187 T6186 arg2Of variance,of
R5152 T6187 T6188 arg1Of variance,(
R5153 T6189 T6188 arg2Of ANOVA,(
R5154 T6190 T6188 arg3Of ),(
R5155 T6194 T6191 arg2Of correction,with
R5156 T6194 T6192 arg1Of correction,a
R5157 T6194 T6193 arg1Of correction,Bonferroni
R5158 T6196 T6195 arg1Of significance,Statistical
R5159 T6196 T6197 arg1Of significance,was
R5160 T6196 T6198 arg2Of significance,defined
R5161 T6198 T6197 arg2Of defined,was
R5162 T6198 T6199 arg1Of defined,as
R5163 T6202 T6199 arg2Of 0.05,as
R5164 T6202 T6200 arg1Of 0.05,P
R5165 T6202 T6201 arg1Of 0.05,<