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PMC:2664230 / 10802-12526 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T5231 0-5 NN denotes NF-κB
T5232 6-9 CC denotes and
T5233 10-14 NN denotes CREB
T5234 15-30 JJ denotes Electrophoretic
T5235 31-39 NN denotes Mobility
T5236 40-45 NN denotes Shift
T5237 46-51 NN denotes Assay
T5238 52-53 -LRB- denotes (
T5239 53-57 NN denotes EMSA
T5240 57-58 -RRB- denotes )
T5241 59-62 DT denotes The
T5242 63-78 JJ denotes non-radioactive
T5243 79-89 NNP denotes LightShift
T5244 90-106 NNP denotes Chemiluminescent
T5245 107-111 NNP denotes EMSA
T5246 112-115 NN denotes Kit
T5247 116-119 VB denotes was
T5248 120-124 VB denotes used
T5249 125-127 TO denotes to
T5250 128-134 VB denotes detect
T5251 135-146 JJ denotes DNA-protein
T5252 147-159 NN denotes interactions
T5253 161-164 DT denotes The
T5254 165-170 NN denotes NF-κB
T5255 171-173 NN denotes 3′
T5256 174-180 NN denotes biotin
T5257 181-192 JJ denotes end-labeled
T5258 193-196 NN denotes DNA
T5259 197-212 NN denotes oligonucleotide
T5260 213-217 VB denotes used
T5261 218-220 IN denotes as
T5262 221-222 DT denotes a
T5263 223-228 NN denotes probe
T5264 229-232 IN denotes for
T5265 233-236 DT denotes the
T5266 237-241 NN denotes EMSA
T5267 242-245 VB denotes was
T5268 246-247 DT denotes a
T5269 248-253 JJ denotes 42-bp
T5270 254-260 JJ denotes double
T5271 261-269 JJ denotes stranded
T5272 270-279 NN denotes construct
T5273 280-281 -LRB- denotes (
T5274 281-327 NN denotes 5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
T5275 328-329 NN denotes –
T5276 330-332 NN denotes 3′
T5277 332-333 -RRB- denotes )
T5278 334-344 VB denotes containing
T5279 345-348 CD denotes two
T5280 349-357 RB denotes tandemly
T5281 358-366 VB denotes repeated
T5282 367-372 NN denotes NF-κB
T5283 373-380 NN denotes binding
T5284 381-386 NN denotes sites
T5285 387-388 -LRB- denotes (
T5286 388-398 VB denotes underlined
T5287 398-399 -RRB- denotes )
T5288 401-412 NN denotes Specificity
T5289 413-416 VB denotes was
T5290 417-427 VB denotes determined
T5291 428-430 IN denotes by
T5292 431-432 DT denotes a
T5293 433-444 NN denotes competition
T5294 445-450 NN denotes assay
T5295 451-455 IN denotes with
T5296 456-459 DT denotes the
T5297 460-468 NN denotes addition
T5298 469-471 IN denotes of
T5299 472-475 CD denotes 200
T5300 476-481 JJ denotes molar
T5301 482-488 NN denotes excess
T5302 489-491 IN denotes of
T5303 492-501 JJ denotes unlabeled
T5304 502-508 JJ denotes double
T5305 509-517 JJ denotes stranded
T5306 518-523 NN denotes NF-κB
T5307 524-539 NN denotes oligonucleotide
T5308 541-544 DT denotes The
T5309 545-549 NN denotes CREB
T5310 550-552 NN denotes 3′
T5311 553-559 NN denotes biotin
T5312 560-571 JJ denotes end-labeled
T5313 572-575 NN denotes DNA
T5314 576-591 NN denotes oligonucleotide
T5315 592-596 VB denotes used
T5316 597-599 IN denotes as
T5317 600-601 DT denotes a
T5318 602-607 NN denotes probe
T5319 608-611 IN denotes for
T5320 612-615 DT denotes the
T5321 616-620 NN denotes EMSA
T5322 621-624 VB denotes was
T5323 625-626 DT denotes a
T5324 627-632 JJ denotes 23-bp
T5325 633-639 JJ denotes double
T5326 640-648 JJ denotes stranded
T5327 649-658 NN denotes construct
T5328 659-660 -LRB- denotes (
T5329 660-666 NN denotes 5′-TTT
T5330 667-670 NN denotes TCG
T5331 671-674 NN denotes AGC
T5332 675-690 NN denotes TCTGACGTCAGA-GC
T5333 691-692 NN denotes –
T5334 693-695 NN denotes 3′
T5335 695-696 -RRB- denotes )
T5336 697-707 VB denotes containing
T5337 708-711 DT denotes the
T5338 712-715 NN denotes CRE
T5339 716-725 NN denotes consensus
T5340 726-734 NN denotes sequence
T5341 735-736 -LRB- denotes (
T5342 736-746 VB denotes underlined
T5343 746-747 -RRB- denotes )
T5344 749-760 NN denotes Specificity
T5345 761-764 VB denotes was
T5346 765-775 VB denotes determined
T5347 776-778 IN denotes by
T5348 779-780 DT denotes a
T5349 781-792 NN denotes competition
T5350 793-798 NN denotes assay
T5351 799-803 IN denotes with
T5352 804-807 DT denotes the
T5353 808-816 NN denotes addition
T5354 817-819 IN denotes of
T5355 820-823 CD denotes 200
T5356 824-829 JJ denotes molar
T5357 830-836 NN denotes excess
T5358 837-839 IN denotes of
T5359 840-849 JJ denotes unlabeled
T5360 850-856 JJ denotes double
T5361 857-865 JJ denotes stranded
T5362 866-870 NN denotes CREB
T5363 871-886 NN denotes oligonucleotide
T5364 888-895 JJ denotes Nuclear
T5365 896-904 NN denotes extracts
T5366 905-906 -LRB- denotes (
T5367 906-908 CD denotes 10
T5368 909-911 NN denotes μg
T5369 911-912 -RRB- denotes )
T5370 913-917 VB denotes were
T5371 918-927 VB denotes incubated
T5372 928-932 IN denotes with
T5373 933-934 CD denotes 5
T5374 935-937 NN denotes nM
T5375 938-943 NN denotes NF-κB
T5376 944-946 CC denotes or
T5377 947-951 NN denotes CREB
T5378 952-957 NN denotes probe
T5379 958-959 -LRB- denotes (
T5380 959-964 NN denotes NF-kB
T5381 964-965 -COLON- denotes :
T5382 966-968 NN denotes 1x
T5383 969-976 NN denotes binding
T5384 977-983 NN denotes buffer
T5385 983-984 -COMMA- denotes ,
T5386 985-987 CD denotes 50
T5387 988-990 NN denotes mM
T5388 991-994 NN denotes KCl
T5389 994-995 -COMMA- denotes ,
T5390 996-997 CD denotes 1
T5391 998-1000 NN denotes mM
T5392 1001-1005 NN denotes EDTA
T5393 1005-1006 -COMMA- denotes ,
T5394 1007-1008 CD denotes 1
T5395 1009-1011 NN denotes mM
T5396 1012-1015 NN denotes DTT
T5397 1015-1016 -COMMA- denotes ,
T5398 1017-1020 CD denotes 0.1
T5399 1020-1021 NN denotes %
T5400 1022-1026 NN denotes NP40
T5401 1026-1027 -COMMA- denotes ,
T5402 1028-1030 CD denotes 10
T5403 1030-1031 NN denotes %
T5404 1032-1040 NN denotes glycerol
T5405 1040-1041 -COMMA- denotes ,
T5406 1042-1045 CC denotes and
T5407 1046-1048 CD denotes 50
T5408 1049-1054 NN denotes ng/μl
T5409 1055-1059 NN denotes poly
T5410 1060-1065 NN denotes dI-dC
T5411 1065-1066 -COMMA- denotes ,
T5412 1067-1071 NN denotes CREB
T5413 1071-1072 -COLON- denotes :
T5414 1073-1075 NN denotes 1x
T5415 1076-1083 NN denotes binding
T5416 1084-1090 NN denotes buffer
T5417 1090-1091 -COMMA- denotes ,
T5418 1092-1094 CD denotes 20
T5419 1095-1097 NN denotes mM
T5420 1098-1102 NN denotes Tris
T5421 1102-1103 -COMMA- denotes ,
T5422 1104-1106 NN denotes pH
T5423 1107-1110 CD denotes 7.5
T5424 1110-1111 -COMMA- denotes ,
T5425 1112-1114 CD denotes 50
T5426 1115-1117 NN denotes mM
T5427 1118-1121 NN denotes KCl
T5428 1121-1122 -COMMA- denotes ,
T5429 1123-1124 CD denotes 1
T5430 1125-1127 NN denotes mM
T5431 1128-1132 NN denotes EDTA
T5432 1132-1133 -COMMA- denotes ,
T5433 1134-1135 CD denotes 1
T5434 1136-1138 NN denotes mM
T5435 1139-1142 NN denotes DTT
T5436 1142-1143 -COMMA- denotes ,
T5437 1144-1148 CD denotes 0.10
T5438 1148-1149 NN denotes %
T5439 1150-1154 NN denotes NP40
T5440 1154-1155 -COMMA- denotes ,
T5441 1156-1157 CD denotes 6
T5442 1157-1158 NN denotes %
T5443 1159-1167 NN denotes glycerol
T5444 1167-1168 -COMMA- denotes ,
T5445 1169-1172 CD denotes 0.1
T5446 1173-1178 NN denotes mg/mL
T5447 1179-1182 NN denotes BSA
T5448 1183-1186 CC denotes and
T5449 1187-1189 CD denotes 50
T5450 1190-1195 NN denotes ng/μl
T5451 1196-1200 NN denotes poly
T5452 1201-1206 NN denotes dI-dC
T5453 1206-1207 -RRB- denotes )
T5454 1208-1211 CC denotes and
T5455 1212-1216 VB denotes were
T5456 1217-1221 RB denotes then
T5457 1222-1237 VB denotes electrophoresed
T5458 1238-1245 IN denotes through
T5459 1246-1247 DT denotes a
T5460 1248-1249 CD denotes 6
T5461 1249-1250 NN denotes %
T5462 1251-1254 NN denotes DNA
T5463 1255-1266 NN denotes retardation
T5464 1267-1270 NN denotes gel
T5465 1271-1273 IN denotes at
T5466 1274-1278 NN denotes 100V
T5467 1279-1282 IN denotes for
T5468 1283-1285 CD denotes 90
T5469 1286-1293 NN denotes minutes
T5470 1295-1298 DT denotes The
T5471 1299-1303 NN denotes gels
T5472 1304-1308 VB denotes were
T5473 1309-1328 RB denotes electrophoretically
T5474 1329-1340 VB denotes transferred
T5475 1341-1343 IN denotes at
T5476 1344-1349 NN denotes 380mA
T5477 1350-1353 IN denotes for
T5478 1354-1355 CD denotes 1
T5479 1356-1360 NN denotes hour
T5480 1361-1363 IN denotes on
T5481 1364-1367 NN denotes ice
T5482 1368-1370 TO denotes to
T5483 1371-1372 DT denotes a
T5484 1373-1383 RB denotes positively
T5485 1384-1391 VB denotes charged
T5486 1392-1397 NN denotes nylon
T5487 1398-1406 NN denotes membrane
T5488 1407-1410 CC denotes and
T5489 1411-1422 RB denotes immediately
T5490 1423-1435 JJ denotes cross-linked
T5491 1436-1439 IN denotes for
T5492 1440-1442 CD denotes 15
T5493 1443-1450 NN denotes minutes
T5494 1451-1455 IN denotes with
T5495 1456-1457 DT denotes a
T5496 1458-1460 NN denotes UV
T5497 1461-1477 NN denotes transilluminator
T5498 1478-1486 VB denotes equipped
T5499 1487-1491 IN denotes with
T5500 1492-1493 DT denotes a
T5501 1494-1497 CD denotes 312
T5502 1498-1500 NN denotes nm
T5503 1501-1505 NN denotes bulb
T5504 1507-1531 JJ denotes Streptavidin-horseradish
T5505 1532-1542 NN denotes peroxidase
T5506 1543-1552 NN denotes conjugate
T5507 1553-1556 CC denotes and
T5508 1557-1560 DT denotes the
T5509 1561-1571 NNP denotes LightShift
T5510 1572-1588 NNP denotes Chemiluminescent
T5511 1589-1598 NNP denotes Substrate
T5512 1599-1603 VB denotes were
T5513 1604-1608 VB denotes used
T5514 1609-1611 TO denotes to
T5515 1612-1618 VB denotes detect
T5516 1619-1622 DT denotes the
T5517 1623-1629 NN denotes biotin
T5518 1630-1641 JJ denotes end-labeled
T5519 1642-1645 NN denotes DNA
T5520 1647-1650 DT denotes The
T5521 1651-1656 NN denotes nylon
T5522 1657-1666 NN denotes membranes
T5523 1667-1671 VB denotes were
T5524 1672-1679 VB denotes exposed
T5525 1680-1682 TO denotes to
T5526 1683-1688 NN denotes x-ray
T5527 1689-1693 NN denotes film
T5528 1694-1697 IN denotes for
T5529 1698-1701 CD denotes 1–3
T5530 1702-1709 NN denotes minutes
T5531 1710-1713 IN denotes for
T5532 1714-1723 NN denotes detection
R4358 T5231 T5232 arg1Of NF-κB,and
R4359 T5233 T5232 arg2Of CREB,and
R4360 T5237 T5231 arg1Of Assay,NF-κB
R4361 T5237 T5233 arg1Of Assay,CREB
R4362 T5237 T5234 arg1Of Assay,Electrophoretic
R4363 T5237 T5235 arg1Of Assay,Mobility
R4364 T5237 T5236 arg1Of Assay,Shift
R4365 T5237 T5238 arg1Of Assay,(
R4366 T5239 T5238 arg2Of EMSA,(
R4367 T5240 T5238 arg3Of ),(
R4368 T5245 T5243 arg1Of EMSA,LightShift
R4369 T5245 T5244 arg1Of EMSA,Chemiluminescent
R4370 T5246 T5241 arg1Of Kit,The
R4371 T5246 T5242 arg1Of Kit,non-radioactive
R4372 T5246 T5245 arg1Of Kit,EMSA
R4373 T5246 T5247 arg1Of Kit,was
R4374 T5246 T5248 arg2Of Kit,used
R4375 T5248 T5247 arg2Of used,was
R4376 T5248 T5249 modOf used,to
R4377 T5250 T5249 arg1Of detect,to
R4378 T5252 T5250 arg2Of interactions,detect
R4379 T5252 T5251 arg1Of interactions,DNA-protein
R4380 T5259 T5253 arg1Of oligonucleotide,The
R4381 T5259 T5254 arg1Of oligonucleotide,NF-κB
R4382 T5259 T5255 arg1Of oligonucleotide,3′
R4383 T5259 T5256 arg1Of oligonucleotide,biotin
R4384 T5259 T5257 arg1Of oligonucleotide,end-labeled
R4385 T5259 T5258 arg1Of oligonucleotide,DNA
R4386 T5259 T5260 arg2Of oligonucleotide,used
R4387 T5259 T5267 arg1Of oligonucleotide,was
R4388 T5260 T5261 arg1Of used,as
R4389 T5263 T5261 arg2Of probe,as
R4390 T5263 T5262 arg1Of probe,a
R4391 T5263 T5264 arg1Of probe,for
R4392 T5266 T5264 arg2Of EMSA,for
R4393 T5266 T5265 arg1Of EMSA,the
R4394 T5272 T5267 arg2Of construct,was
R4395 T5272 T5268 arg1Of construct,a
R4396 T5272 T5269 arg1Of construct,42-bp
R4397 T5272 T5270 arg1Of construct,double
R4398 T5272 T5271 arg1Of construct,stranded
R4399 T5272 T5273 arg1Of construct,(
R4400 T5272 T5278 arg1Of construct,containing
R4401 T5276 T5273 arg2Of 3′,(
R4402 T5276 T5274 arg1Of 3′,5′-TTGTTACAA-GGGGACTTTCCGCTGGGGACTTTCCAGGGAGGC
R4403 T5276 T5275 arg1Of 3′,–
R4404 T5277 T5273 arg3Of ),(
R4405 T5284 T5278 arg2Of sites,containing
R4406 T5284 T5279 arg1Of sites,two
R4407 T5284 T5280 arg1Of sites,tandemly
R4408 T5284 T5281 arg2Of sites,repeated
R4409 T5284 T5282 arg1Of sites,NF-κB
R4410 T5284 T5283 arg1Of sites,binding
R4411 T5284 T5285 arg1Of sites,(
R4412 T5286 T5285 arg2Of underlined,(
R4413 T5287 T5285 arg3Of ),(
R4414 T5288 T5289 arg1Of Specificity,was
R4415 T5288 T5290 arg2Of Specificity,determined
R4416 T5290 T5289 arg2Of determined,was
R4417 T5294 T5290 arg1Of assay,determined
R4418 T5294 T5291 arg2Of assay,by
R4419 T5294 T5292 arg1Of assay,a
R4420 T5294 T5293 arg1Of assay,competition
R4421 T5294 T5295 arg1Of assay,with
R4422 T5297 T5295 arg2Of addition,with
R4423 T5297 T5296 arg1Of addition,the
R4424 T5297 T5298 arg1Of addition,of
R4425 T5300 T5299 arg1Of molar,200
R4426 T5301 T5298 arg2Of excess,of
R4427 T5301 T5300 arg1Of excess,molar
R4428 T5301 T5302 arg1Of excess,of
R4429 T5305 T5303 arg1Of stranded,unlabeled
R4430 T5305 T5304 arg1Of stranded,double
R4431 T5307 T5302 arg2Of oligonucleotide,of
R4432 T5307 T5305 arg1Of oligonucleotide,stranded
R4433 T5307 T5306 arg1Of oligonucleotide,NF-κB
R4434 T5314 T5308 arg1Of oligonucleotide,The
R4435 T5314 T5309 arg1Of oligonucleotide,CREB
R4436 T5314 T5310 arg1Of oligonucleotide,3′
R4437 T5314 T5311 arg1Of oligonucleotide,biotin
R4438 T5314 T5312 arg1Of oligonucleotide,end-labeled
R4439 T5314 T5313 arg1Of oligonucleotide,DNA
R4440 T5314 T5315 arg2Of oligonucleotide,used
R4441 T5314 T5322 arg1Of oligonucleotide,was
R4442 T5315 T5316 arg1Of used,as
R4443 T5318 T5316 arg2Of probe,as
R4444 T5318 T5317 arg1Of probe,a
R4445 T5318 T5319 arg1Of probe,for
R4446 T5321 T5319 arg2Of EMSA,for
R4447 T5321 T5320 arg1Of EMSA,the
R4448 T5327 T5322 arg2Of construct,was
R4449 T5327 T5323 arg1Of construct,a
R4450 T5327 T5324 arg1Of construct,23-bp
R4451 T5327 T5325 arg1Of construct,double
R4452 T5327 T5326 arg1Of construct,stranded
R4453 T5327 T5328 arg1Of construct,(
R4454 T5327 T5336 arg1Of construct,containing
R4455 T5334 T5328 arg2Of 3′,(
R4456 T5334 T5329 arg1Of 3′,5′-TTT
R4457 T5334 T5330 arg1Of 3′,TCG
R4458 T5334 T5331 arg1Of 3′,AGC
R4459 T5334 T5332 arg1Of 3′,TCTGACGTCAGA-GC
R4460 T5334 T5333 arg1Of 3′,–
R4461 T5335 T5328 arg3Of ),(
R4462 T5340 T5336 arg2Of sequence,containing
R4463 T5340 T5337 arg1Of sequence,the
R4464 T5340 T5338 arg1Of sequence,CRE
R4465 T5340 T5339 arg1Of sequence,consensus
R4466 T5340 T5341 arg1Of sequence,(
R4467 T5342 T5341 arg2Of underlined,(
R4468 T5343 T5341 arg3Of ),(
R4469 T5344 T5345 arg1Of Specificity,was
R4470 T5344 T5346 arg2Of Specificity,determined
R4471 T5346 T5345 arg2Of determined,was
R4472 T5350 T5346 arg1Of assay,determined
R4473 T5350 T5347 arg2Of assay,by
R4474 T5350 T5348 arg1Of assay,a
R4475 T5350 T5349 arg1Of assay,competition
R4476 T5350 T5351 arg1Of assay,with
R4477 T5353 T5351 arg2Of addition,with
R4478 T5353 T5352 arg1Of addition,the
R4479 T5353 T5354 arg1Of addition,of
R4480 T5356 T5355 arg1Of molar,200
R4481 T5357 T5354 arg2Of excess,of
R4482 T5357 T5356 arg1Of excess,molar
R4483 T5357 T5358 arg1Of excess,of
R4484 T5361 T5359 arg1Of stranded,unlabeled
R4485 T5361 T5360 arg1Of stranded,double
R4486 T5363 T5358 arg2Of oligonucleotide,of
R4487 T5363 T5361 arg1Of oligonucleotide,stranded
R4488 T5363 T5362 arg1Of oligonucleotide,CREB
R4489 T5365 T5364 arg1Of extracts,Nuclear
R4490 T5365 T5366 arg1Of extracts,(
R4491 T5365 T5370 arg1Of extracts,were
R4492 T5365 T5371 arg2Of extracts,incubated
R4493 T5368 T5366 arg2Of μg,(
R4494 T5368 T5367 arg1Of μg,10
R4495 T5369 T5366 arg3Of ),(
R4496 T5371 T5370 arg2Of incubated,were
R4497 T5371 T5372 arg1Of incubated,with
R4498 T5373 T5374 arg1Of 5,nM
R4499 T5375 T5373 arg1Of NF-κB,5
R4500 T5375 T5376 arg1Of NF-κB,or
R4501 T5376 T5372 arg2Of or,with
R4502 T5378 T5376 arg2Of probe,or
R4503 T5378 T5377 arg1Of probe,CREB
R4504 T5378 T5379 arg1Of probe,(
R4505 T5380 T5379 arg2Of NF-kB,(
R4506 T5381 T5379 arg3Of :,(
R4507 T5384 T5382 arg1Of buffer,1x
R4508 T5384 T5383 arg1Of buffer,binding
R4509 T5384 T5385 arg1Of buffer,","
R4510 T5385 T5389 arg1Of ",",","
R4511 T5386 T5387 arg1Of 50,mM
R4512 T5388 T5385 arg2Of KCl,","
R4513 T5388 T5386 arg1Of KCl,50
R4514 T5389 T5393 arg1Of ",",","
R4515 T5390 T5391 arg1Of 1,mM
R4516 T5392 T5389 arg2Of EDTA,","
R4517 T5392 T5390 arg1Of EDTA,1
R4518 T5393 T5397 arg1Of ",",","
R4519 T5394 T5395 arg1Of 1,mM
R4520 T5396 T5393 arg2Of DTT,","
R4521 T5396 T5394 arg1Of DTT,1
R4522 T5397 T5401 arg1Of ",",","
R4523 T5398 T5399 arg1Of 0.1,%
R4524 T5400 T5397 arg2Of NP40,","
R4525 T5400 T5398 arg1Of NP40,0.1
R4526 T5401 T5406 arg1Of ",",and
R4527 T5403 T5402 arg1Of %,10
R4528 T5404 T5401 arg2Of glycerol,","
R4529 T5404 T5403 arg1Of glycerol,%
R4530 T5406 T5405 arg1Of and,","
R4531 T5410 T5406 arg2Of dI-dC,and
R4532 T5410 T5407 arg1Of dI-dC,50
R4533 T5410 T5408 arg1Of dI-dC,ng/μl
R4534 T5410 T5409 arg1Of dI-dC,poly
R4535 T5410 T5411 arg1Of dI-dC,","
R4536 T5412 T5411 arg2Of CREB,","
R4537 T5412 T5413 arg1Of CREB,:
R4538 T5416 T5414 arg1Of buffer,1x
R4539 T5416 T5415 arg1Of buffer,binding
R4540 T5416 T5417 arg1Of buffer,","
R4541 T5418 T5419 arg1Of 20,mM
R4542 T5420 T5417 arg2Of Tris,","
R4543 T5420 T5418 arg1Of Tris,20
R4544 T5420 T5421 arg1Of Tris,","
R4545 T5422 T5421 arg2Of pH,","
R4546 T5422 T5423 arg1Of pH,7.5
R4547 T5425 T5426 arg1Of 50,mM
R4548 T5427 T5425 arg1Of KCl,50
R4549 T5427 T5428 arg1Of KCl,","
R4550 T5428 T5432 arg1Of ",",","
R4551 T5429 T5430 arg1Of 1,mM
R4552 T5431 T5428 arg2Of EDTA,","
R4553 T5431 T5429 arg1Of EDTA,1
R4554 T5433 T5434 arg1Of 1,mM
R4555 T5435 T5432 arg2Of DTT,","
R4556 T5435 T5433 arg1Of DTT,1
R4557 T5437 T5438 arg1Of 0.10,%
R4558 T5439 T5437 arg1Of NP40,0.10
R4559 T5439 T5440 arg1Of NP40,","
R4560 T5440 T5444 arg1Of ",",","
R4561 T5441 T5442 arg1Of 6,%
R4562 T5443 T5440 arg2Of glycerol,","
R4563 T5443 T5441 arg1Of glycerol,6
R4564 T5444 T5448 arg1Of ",",and
R4565 T5445 T5446 arg1Of 0.1,mg/mL
R4566 T5447 T5444 arg2Of BSA,","
R4567 T5447 T5445 arg1Of BSA,0.1
R4568 T5452 T5448 arg2Of dI-dC,and
R4569 T5452 T5449 arg1Of dI-dC,50
R4570 T5452 T5450 arg1Of dI-dC,ng/μl
R4571 T5452 T5451 arg1Of dI-dC,poly
R4572 T5457 T5454 arg2Of electrophoresed,and
R4573 T5457 T5455 arg2Of electrophoresed,were
R4574 T5457 T5456 arg1Of electrophoresed,then
R4575 T5457 T5458 arg1Of electrophoresed,through
R4576 T5460 T5461 arg1Of 6,%
R4577 T5464 T5458 arg2Of gel,through
R4578 T5464 T5459 arg1Of gel,a
R4579 T5464 T5460 arg1Of gel,6
R4580 T5464 T5462 arg1Of gel,DNA
R4581 T5464 T5463 arg1Of gel,retardation
R4582 T5464 T5465 arg1Of gel,at
R4583 T5466 T5465 arg2Of 100V,at
R4584 T5466 T5467 arg1Of 100V,for
R4585 T5469 T5467 arg2Of minutes,for
R4586 T5469 T5468 arg1Of minutes,90
R4587 T5471 T5470 arg1Of gels,The
R4588 T5471 T5472 arg1Of gels,were
R4589 T5471 T5474 arg2Of gels,transferred
R4590 T5474 T5472 arg2Of transferred,were
R4591 T5474 T5473 arg1Of transferred,electrophoretically
R4592 T5474 T5475 arg1Of transferred,at
R4593 T5476 T5475 arg2Of 380mA,at
R4594 T5476 T5477 arg1Of 380mA,for
R4595 T5476 T5489 arg1Of 380mA,immediately
R4596 T5477 T5488 arg1Of for,and
R4597 T5479 T5477 arg2Of hour,for
R4598 T5479 T5478 arg1Of hour,1
R4599 T5479 T5480 arg1Of hour,on
R4600 T5479 T5482 arg1Of hour,to
R4601 T5481 T5480 arg2Of ice,on
R4602 T5485 T5484 arg1Of charged,positively
R4603 T5487 T5482 arg2Of membrane,to
R4604 T5487 T5483 arg1Of membrane,a
R4605 T5487 T5485 arg1Of membrane,charged
R4606 T5487 T5486 arg1Of membrane,nylon
R4607 T5489 T5488 arg2Of immediately,and
R4608 T5489 T5490 arg1Of immediately,cross-linked
R4609 T5490 T5491 arg1Of cross-linked,for
R4610 T5493 T5491 arg2Of minutes,for
R4611 T5493 T5492 arg1Of minutes,15
R4612 T5493 T5494 arg1Of minutes,with
R4613 T5497 T5494 arg2Of transilluminator,with
R4614 T5497 T5495 arg1Of transilluminator,a
R4615 T5497 T5496 arg1Of transilluminator,UV
R4616 T5497 T5498 arg2Of transilluminator,equipped
R4617 T5498 T5499 arg1Of equipped,with
R4618 T5501 T5502 arg1Of 312,nm
R4619 T5503 T5499 arg2Of bulb,with
R4620 T5503 T5500 arg1Of bulb,a
R4621 T5503 T5501 arg1Of bulb,312
R4622 T5506 T5504 arg1Of conjugate,Streptavidin-horseradish
R4623 T5506 T5505 arg1Of conjugate,peroxidase
R4624 T5506 T5507 arg1Of conjugate,and
R4625 T5507 T5512 arg1Of and,were
R4626 T5507 T5513 arg2Of and,used
R4627 T5511 T5507 arg2Of Substrate,and
R4628 T5511 T5508 arg1Of Substrate,the
R4629 T5511 T5509 arg1Of Substrate,LightShift
R4630 T5511 T5510 arg1Of Substrate,Chemiluminescent
R4631 T5513 T5512 arg2Of used,were
R4632 T5515 T5513 arg3Of detect,used
R4633 T5515 T5514 arg1Of detect,to
R4634 T5519 T5515 arg2Of DNA,detect
R4635 T5519 T5516 arg1Of DNA,the
R4636 T5519 T5517 arg1Of DNA,biotin
R4637 T5519 T5518 arg1Of DNA,end-labeled
R4638 T5522 T5520 arg1Of membranes,The
R4639 T5522 T5521 arg1Of membranes,nylon
R4640 T5522 T5523 arg1Of membranes,were
R4641 T5522 T5524 arg2Of membranes,exposed
R4642 T5524 T5523 arg2Of exposed,were
R4643 T5524 T5525 arg1Of exposed,to
R4644 T5524 T5528 arg1Of exposed,for
R4645 T5527 T5525 arg2Of film,to
R4646 T5527 T5526 arg1Of film,x-ray
R4647 T5530 T5528 arg2Of minutes,for
R4648 T5530 T5529 arg1Of minutes,1–3
R4649 T5530 T5531 arg1Of minutes,for
R4650 T5532 T5531 arg2Of detection,for