PMC:2626671 JSON TXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T270	0-5	NN	denotes	Runx3
T271	6-9	CC	denotes	and
T272	10-15	NN	denotes	T-box
T273	16-24	NN	denotes	proteins
T274	25-34	VB	denotes	cooperate
T275	35-37	TO	denotes	to
T276	38-47	VB	denotes	establish
T277	48-51	DT	denotes	the
T278	52-67	JJ	denotes	transcriptional
T279	68-75	NN	denotes	program
T280	76-78	IN	denotes	of
T281	79-87	NN	denotes	effector
T282	88-92	NN	denotes	CTLs
T283	104-114	NN	denotes	Activation
T284	115-117	IN	denotes	of
T285	118-123	JJ	denotes	naive
T286	124-128	JJ	denotes	CD8+
T287	129-130	NN	denotes	T
T288	131-136	NN	denotes	cells
T289	137-141	IN	denotes	with
T290	142-149	NN	denotes	antigen
T291	150-157	VB	denotes	induces
T292	158-163	PRP-DOLLAR-	denotes	their
T293	164-179	NN	denotes	differentiation
T294	180-184	IN	denotes	into
T295	185-193	NN	denotes	effector
T296	194-203	JJ	denotes	cytolytic
T297	204-205	NN	denotes	T
T298	206-217	NN	denotes	lymphocytes
T299	218-219	-LRB-	denotes	(
T300	219-223	NN	denotes	CTLs
T301	223-224	-RRB-	denotes	)
T302	226-230	NN	denotes	CTLs
T303	231-235	VB	denotes	lyse
T304	236-244	JJ	denotes	infected
T305	245-247	CC	denotes	or
T306	248-256	JJ	denotes	aberrant
T307	257-263	NN	denotes	target
T308	264-269	NN	denotes	cells
T309	270-272	IN	denotes	by
T310	273-283	NN	denotes	exocytosis
T311	284-286	IN	denotes	of
T312	287-292	JJ	denotes	lytic
T313	293-301	NN	denotes	granules
T314	302-312	VB	denotes	containing
T315	313-316	DT	denotes	the
T316	317-329	JJ	denotes	pore-forming
T317	330-337	NN	denotes	protein
T318	338-346	NN	denotes	perforin
T319	347-350	CC	denotes	and
T320	351-352	DT	denotes	a
T321	353-359	NN	denotes	family
T322	360-362	IN	denotes	of
T323	363-372	NN	denotes	proteases
T324	373-379	VB	denotes	termed
T325	380-389	NN	denotes	granzymes
T326	391-393	PRP	denotes	We
T327	394-398	VB	denotes	show
T328	399-403	IN	denotes	that
T329	404-412	NN	denotes	effector
T330	413-416	NN	denotes	CTL
T331	417-432	NN	denotes	differentiation
T332	433-439	VB	denotes	occurs
T333	440-442	IN	denotes	in
T334	443-446	CD	denotes	two
T335	447-457	JJ	denotes	sequential
T336	458-464	NN	denotes	phases
T337	465-467	FW	denotes	in
T338	468-473	FW	denotes	vitro
T339	473-474	-COMMA-	denotes	,
T340	475-488	VB	denotes	characterized
T341	489-491	IN	denotes	by
T342	492-497	JJ	denotes	early
T343	498-507	NN	denotes	induction
T344	508-510	IN	denotes	of
T345	511-516	NN	denotes	T-bet
T346	517-520	CC	denotes	and
T347	521-525	JJ	denotes	late
T348	526-535	NN	denotes	induction
T349	536-538	IN	denotes	of
T350	539-551	NN	denotes	Eomesodermin
T351	552-553	-LRB-	denotes	(
T352	553-558	NN	denotes	Eomes
T353	558-559	-RRB-	denotes	)
T354	559-560	-COMMA-	denotes	,
T355	561-566	NN	denotes	T-box
T356	567-580	NN	denotes	transcription
T357	581-588	NN	denotes	factors
T358	589-593	WDT	denotes	that
T359	594-602	VB	denotes	regulate
T360	603-606	DT	denotes	the
T361	607-612	JJ	denotes	early
T362	613-616	CC	denotes	and
T363	617-621	JJ	denotes	late
T364	622-628	NN	denotes	phases
T365	629-631	IN	denotes	of
T366	632-642	NN	denotes	interferon
T367	643-644	-LRB-	denotes	(
T368	644-647	NN	denotes	IFN
T369	647-648	-RRB-	denotes	)
T370	649-650	NN	denotes	γ
T371	651-661	NN	denotes	expression
T372	661-662	-COMMA-	denotes	,
T373	663-675	RB	denotes	respectively
T374	677-679	IN	denotes	In
T375	680-688	NN	denotes	addition
T376	688-689	-COMMA-	denotes	,
T377	690-692	PRP	denotes	we
T378	693-704	VB	denotes	demonstrate
T379	705-706	DT	denotes	a
T380	707-715	JJ	denotes	critical
T381	716-720	NN	denotes	role
T382	721-724	IN	denotes	for
T383	725-728	DT	denotes	the
T384	729-742	NN	denotes	transcription
T385	743-749	NN	denotes	factor
T386	750-755	NN	denotes	Runx3
T387	756-758	IN	denotes	in
T388	759-762	NN	denotes	CTL
T389	763-778	NN	denotes	differentiation
T390	780-785	NN	denotes	Runx3
T391	786-795	VB	denotes	regulates
T392	796-801	NN	denotes	Eomes
T393	802-812	NN	denotes	expression
T394	813-815	RB	denotes	as
T395	816-820	RB	denotes	well
T396	821-823	IN	denotes	as
T397	824-834	NN	denotes	expression
T398	835-837	IN	denotes	of
T399	838-843	CD	denotes	three
T400	844-852	JJ	denotes	cardinal
T401	853-860	NN	denotes	markers
T402	861-863	IN	denotes	of
T403	864-867	DT	denotes	the
T404	868-876	NN	denotes	effector
T405	877-880	NN	denotes	CTL
T406	881-888	NN	denotes	program
T407	888-889	-COLON-	denotes	:
T408	890-895	NN	denotes	IFN-γ
T409	895-896	-COMMA-	denotes	,
T410	897-905	NN	denotes	perforin
T411	905-906	-COMMA-	denotes	,
T412	907-910	CC	denotes	and
T413	911-919	NN	denotes	granzyme
T414	920-922	NNP	denotes	B.
T415	923-926	PRP-DOLLAR-	denotes	Our
T416	927-931	NN	denotes	data
T417	932-937	VB	denotes	point
T418	938-940	TO	denotes	to
T419	941-944	DT	denotes	the
T420	945-954	NN	denotes	existence
T421	955-957	IN	denotes	of
T422	958-960	DT	denotes	an
T423	961-970	JJ	denotes	elaborate
T424	971-986	JJ	denotes	transcriptional
T425	987-994	NN	denotes	network
T426	995-997	IN	denotes	in
T427	998-1003	WDT	denotes	which
T428	1004-1009	NN	denotes	Runx3
T429	1010-1019	RB	denotes	initially
T430	1020-1027	VB	denotes	induces
T431	1028-1031	CC	denotes	and
T432	1032-1036	RB	denotes	then
T433	1037-1047	VB	denotes	cooperates
T434	1048-1052	IN	denotes	with
T435	1053-1058	NN	denotes	T-box
T436	1059-1072	NN	denotes	transcription
T437	1073-1080	NN	denotes	factors
T438	1081-1083	TO	denotes	to
T439	1084-1092	VB	denotes	regulate
T440	1093-1097	NN	denotes	gene
T441	1098-1111	NN	denotes	transcription
T442	1112-1114	IN	denotes	in
T443	1115-1130	VB	denotes	differentiating
T444	1131-1135	NN	denotes	CTLs
T1503	1138-1143	JJ	denotes	Naive
T1504	1144-1150	NN	denotes	CD8+ T
T1505	1151-1156	NN	denotes	cells
T1506	1157-1170	VB	denotes	differentiate
T1507	1171-1175	IN	denotes	into
T1508	1176-1184	NN	denotes	effector
T1509	1185-1189	NN	denotes	CTLs
T1510	1190-1194	IN	denotes	with
T1511	1195-1198	DT	denotes	the
T1512	1199-1206	NN	denotes	ability
T1513	1207-1209	TO	denotes	to
T1514	1210-1214	VB	denotes	lyse
T1515	1215-1230	JJ	denotes	antigen-bearing
T1516	1231-1237	NN	denotes	target
T1517	1238-1243	NN	denotes	cells
T1518	1244-1246	IN	denotes	by
T1519	1247-1257	NN	denotes	exocytosis
T1520	1258-1260	IN	denotes	of
T1521	1261-1266	JJ	denotes	lytic
T1522	1267-1275	NN	denotes	granules
T1523	1276-1286	VB	denotes	containing
T1524	1287-1295	NN	denotes	perforin
T1525	1296-1299	CC	denotes	and
T1526	1300-1309	NN	denotes	granzymes
T1527	1309-1310	-COMMA-	denotes	,
T1528	1311-1314	CC	denotes	and
T1529	1315-1317	TO	denotes	to
T1530	1318-1325	VB	denotes	produce
T1531	1326-1338	JJ	denotes	inflammatory
T1532	1339-1348	NN	denotes	cytokines
T1533	1349-1353	JJ	denotes	such
T1534	1354-1356	IN	denotes	as
T1535	1357-1362	NN	denotes	IFN-γ
T1536	1363-1366	CC	denotes	and
T1537	1367-1370	NN	denotes	TNF
T1538	1371-1375	IN	denotes	upon
T1539	1376-1389	NN	denotes	restimulation
T1540	1390-1397	IN	denotes	through
T1541	1398-1401	DT	denotes	the
T1542	1402-1405	NN	denotes	TCR
T1543	1406-1407	-LRB-	denotes	(
T1544	1407-1411	CD	denotes	1, 2
T1545	1411-1412	-RRB-	denotes	)
T1546	1414-1416	FW	denotes	In
T1547	1417-1421	FW	denotes	vivo
T1548	1422-1433	NN	denotes	experiments
T1549	1434-1438	VB	denotes	have
T1550	1439-1449	VB	denotes	elucidated
T1551	1450-1454	JJ	denotes	many
T1552	1455-1463	JJ	denotes	critical
T1553	1464-1474	NN	denotes	parameters
T1554	1475-1484	VB	denotes	governing
T1555	1485-1488	DT	denotes	the
T1556	1489-1500	NN	denotes	development
T1557	1501-1504	CC	denotes	and
T1558	1505-1514	NN	denotes	evolution
T1559	1515-1517	IN	denotes	of
T1560	1518-1525	JJ	denotes	primary
T1561	1526-1529	NN	denotes	CTL
T1562	1530-1539	NN	denotes	responses
T1563	1540-1541	-LRB-	denotes	(
T1564	1541-1545	CD	denotes	3, 4
T1565	1545-1546	-RRB-	denotes	)
T1566	1548-1550	IN	denotes	In
T1567	1551-1555	DT	denotes	this
T1568	1556-1561	NN	denotes	study
T1569	1561-1562	-COMMA-	denotes	,
T1570	1563-1565	PRP	denotes	we
T1571	1566-1570	VB	denotes	have
T1572	1571-1575	VB	denotes	used
T1573	1576-1578	FW	denotes	in
T1574	1579-1584	FW	denotes	vitro
T1575	1585-1592	NN	denotes	systems
T1576	1593-1597	JJ	denotes	such
T1577	1598-1600	IN	denotes	as
T1578	1601-1606	DT	denotes	those
T1579	1607-1616	VB	denotes	developed
T1580	1617-1619	TO	denotes	to
T1581	1620-1625	VB	denotes	study
T1582	1626-1632	NN	denotes	CD4+ T
T1583	1633-1637	NN	denotes	cell
T1584	1638-1653	NN	denotes	differentiation
T1585	1654-1656	TO	denotes	to
T1586	1657-1663	VB	denotes	define
T1587	1664-1667	DT	denotes	the
T1588	1668-1677	JJ	denotes	molecular
T1589	1678-1683	NN	denotes	basis
T1590	1684-1686	IN	denotes	of
T1591	1687-1695	NN	denotes	effector
T1592	1696-1699	NN	denotes	CTL
T1593	1700-1715	NN	denotes	differentiation
T1594	1716-1717	-LRB-	denotes	(
T1595	1717-1721	NN	denotes	5, 6
T1596	1721-1722	-RRB-	denotes	)
T1597	1722-1727	NN	denotes	. The
T1598	1728-1733	NN	denotes	T-box
T1599	1734-1747	NN	denotes	transcription
T1600	1748-1755	NN	denotes	factors
T1601	1756-1768	NN	denotes	Eomesodermin
T1602	1769-1770	-LRB-	denotes	(
T1603	1770-1775	NN	denotes	Eomes
T1604	1775-1776	-RRB-	denotes	)
T1605	1777-1780	CC	denotes	and
T1606	1781-1786	NN	denotes	T-bet
T1607	1787-1790	VB	denotes	are
T1608	1791-1797	VB	denotes	needed
T1609	1798-1801	IN	denotes	for
T1610	1802-1811	JJ	denotes	important
T1611	1812-1819	NN	denotes	aspects
T1612	1820-1822	IN	denotes	of
T1613	1823-1831	NN	denotes	effector
T1614	1832-1835	CC	denotes	and
T1615	1836-1842	NN	denotes	memory
T1616	1843-1846	NN	denotes	CTL
T1617	1847-1862	NN	denotes	differentiation
T1618	1863-1864	-LRB-	denotes	(
T1619	1864-1865	CD	denotes	7
T1620	1865-1866	-RRB-	denotes	)
T1621	1868-1870	IN	denotes	In
T1622	1871-1881	JJ	denotes	uninfected
T1623	1882-1886	NN	denotes	mice
T1624	1886-1887	-COMMA-	denotes	,
T1625	1888-1896	NN	denotes	compound
T1626	1897-1905	NN	denotes	deletion
T1627	1906-1908	IN	denotes	of
T1628	1909-1918	NN	denotes	the Tbx21
T1629	1919-1920	-LRB-	denotes	(
T1630	1920-1928	VB	denotes	encoding
T1631	1929-1934	NN	denotes	T-bet
T1632	1934-1935	-RRB-	denotes	)
T1633	1936-1958	NN	denotes	and eomesodermin genes
T1634	1959-1961	VB	denotes	is
T1635	1962-1972	VB	denotes	associated
T1636	1973-1977	IN	denotes	with
T1637	1978-1979	DT	denotes	a
T1638	1980-1989	JJ	denotes	selective
T1639	1990-1994	NN	denotes	loss
T1640	1995-1997	IN	denotes	of
T1641	1998-2004	NN	denotes	CD8+ T
T1642	2005-2010	NN	denotes	cells
T1643	2011-2015	IN	denotes	with
T1644	2016-2018	DT	denotes	an
T1645	2019-2030	NN	denotes	IL-2Rβ–high
T1646	2030-2031	-COMMA-	denotes	,
T1647	2032-2038	NN	denotes	memory
T1648	2039-2048	NN	denotes	phenotype
T1649	2049-2050	-LRB-	denotes	(
T1650	2050-2051	CD	denotes	8
T1651	2051-2052	-RRB-	denotes	)
T1652	2054-2058	NN	denotes	Mice
T1653	2059-2068	JJ	denotes	deficient
T1654	2069-2072	IN	denotes	for
T1655	2073-2077	CC	denotes	both
T1656	2078-2083	NN	denotes	T-bet
T1657	2084-2087	CC	denotes	and
T1658	2088-2093	NN	denotes	Eomes
T1659	2094-2096	IN	denotes	in
T1660	2097-2098	NN	denotes	T
T1661	2099-2104	NN	denotes	cells
T1662	2105-2109	VB	denotes	have
T1663	2110-2118	VB	denotes	impaired
T1664	2119-2129	NN	denotes	expression
T1665	2130-2132	IN	denotes	of
T1666	2133-2142	JJ	denotes	cytolytic
T1667	2143-2152	NN	denotes	mediators
T1668	2152-2153	-COMMA-	denotes	,
T1669	2154-2162	VB	denotes	manifest
T1670	2163-2167	JJ	denotes	poor
T1671	2168-2177	JJ	denotes	cytolytic
T1672	2178-2186	NN	denotes	activity
T1673	2186-2187	-COMMA-	denotes	,
T1674	2188-2191	CC	denotes	and
T1675	2192-2196	VB	denotes	fail
T1676	2197-2199	TO	denotes	to
T1677	2200-2207	VB	denotes	control
T1678	2208-2213	JJ	denotes	acute
T1679	2214-2225	JJ	denotes	lymphocytic
T1680	2226-2242	NN	denotes	choriomeningitis
T1681	2243-2248	NN	denotes	virus
T1682	2249-2258	NN	denotes	infection
T1683	2259-2260	-LRB-	denotes	(
T1684	2260-2261	CD	denotes	9
T1685	2261-2262	-RRB-	denotes	)
T1686	2264-2276	RB	denotes	Nevertheless
T1687	2276-2277	-COMMA-	denotes	,
T1688	2278-2281	DT	denotes	the
T1689	2282-2290	JJ	denotes	specific
T1690	2291-2296	NN	denotes	roles
T1691	2297-2299	IN	denotes	of
T1692	2300-2305	NN	denotes	T-bet
T1693	2306-2309	CC	denotes	and
T1694	2310-2315	NN	denotes	Eomes
T1695	2316-2318	IN	denotes	in
T1696	2319-2325	JJ	denotes	clonal
T1697	2326-2335	NN	denotes	expansion
T1698	2336-2339	CC	denotes	and
T1699	2340-2343	NN	denotes	CTL
T1700	2344-2359	NN	denotes	differentiation
T1701	2360-2364	VB	denotes	have
T1702	2365-2368	RB	denotes	not
T1703	2369-2372	RB	denotes	yet
T1704	2373-2377	VB	denotes	been
T1705	2378-2386	VB	denotes	resolved
T1706	2386-2387	-COLON-	denotes	:
T1707	2388-2390	IN	denotes	in
T1708	2391-2401	JJ	denotes	particular
T1709	2401-2402	-COMMA-	denotes	,
T1710	2403-2405	PRP	denotes	it
T1711	2406-2408	VB	denotes	is
T1712	2409-2412	RB	denotes	not
T1713	2413-2418	VB	denotes	known
T1714	2419-2426	IN	denotes	whether
T1715	2427-2432	DT	denotes	these
T1716	2433-2446	NN	denotes	transcription
T1717	2447-2454	NN	denotes	factors
T1718	2455-2463	VB	denotes	function
T1719	2464-2475	RB	denotes	redundantly
T1720	2476-2478	TO	denotes	to
T1721	2479-2486	VB	denotes	control
T1722	2487-2495	NN	denotes	effector
T1723	2496-2502	NN	denotes	CD8+ T
T1724	2503-2507	NN	denotes	cell
T1725	2508-2523	NN	denotes	differentiation
T1726	2523-2524	-COMMA-	denotes	,
T1727	2525-2528	CC	denotes	and
T1728	2529-2536	IN	denotes	whether
T1729	2537-2541	PRP	denotes	they
T1730	2542-2544	VB	denotes	do
T1731	2545-2547	RB	denotes	so
T1732	2548-2556	RB	denotes	directly
T1733	2557-2559	IN	denotes	by
T1734	2560-2569	VB	denotes	targeting
T1735	2570-2578	JJ	denotes	specific
T1736	2579-2587	NN	denotes	effector
T1737	2588-2596	NN	denotes	cytokine
T1738	2597-2600	CC	denotes	and
T1739	2601-2610	JJ	denotes	cytolytic
T1740	2611-2622	NN	denotes	genes. Runx
T1741	2623-2631	NN	denotes	proteins
T1742	2631-2632	-COMMA-	denotes	,
T1743	2633-2634	DT	denotes	a
T1744	2635-2641	NN	denotes	family
T1745	2642-2644	IN	denotes	of
T1746	2645-2650	CD	denotes	three
T1747	2651-2662	JJ	denotes	DNA-binding
T1748	2663-2676	NN	denotes	transcription
T1749	2677-2684	NN	denotes	factors
T1750	2684-2685	-COMMA-	denotes	,
T1751	2686-2693	NN	denotes	control
T1752	2694-2703	NN	denotes	thymocyte
T1753	2704-2719	NN	denotes	differentiation
T1754	2720-2723	CC	denotes	and
T1755	2724-2727	DT	denotes	the
T1756	2728-2735	NN	denotes	CD4/CD8
T1757	2736-2743	NN	denotes	lineage
T1758	2744-2752	NN	denotes	decision
T1759	2753-2754	-LRB-	denotes	(
T1760	2754-2759	CD	denotes	10–13
T1761	2759-2760	-RRB-	denotes	)
T1762	2762-2767	NN	denotes	Runx3
T1763	2768-2771	CC	denotes	and
T1764	2772-2780	NN	denotes	perforin
T1765	2781-2785	NN	denotes	mRNA
T1766	2786-2789	VB	denotes	are
T1767	2790-2799	VB	denotes	expressed
T1768	2800-2802	IN	denotes	by
T1769	2803-2818	JJ	denotes	double-positive
T1770	2819-2820	-LRB-	denotes	(
T1771	2820-2822	NN	denotes	DP
T1772	2822-2823	-RRB-	denotes	)
T1773	2824-2834	NN	denotes	thymocytes
T1774	2835-2838	CC	denotes	and
T1775	2839-2859	JJ	denotes	CD8+ single-positive
T1776	2860-2861	-LRB-	denotes	(
T1777	2861-2863	NN	denotes	SP
T1778	2863-2864	-RRB-	denotes	)
T1779	2865-2875	NN	denotes	thymocytes
T1780	2876-2879	CC	denotes	but
T1781	2880-2883	RB	denotes	not
T1782	2884-2886	IN	denotes	in
T1783	2887-2894	NN	denotes	CD4+ SP
T1784	2895-2900	NN	denotes	cells
T1785	2901-2902	-LRB-	denotes	(
T1786	2902-2904	CD	denotes	14
T1787	2904-2905	-RRB-	denotes	)
T1788	2907-2915	IN	denotes	Although
T1789	2916-2921	NN	denotes	Runx3
T1790	2922-2924	VB	denotes	is
T1791	2925-2928	RB	denotes	not
T1792	2929-2938	VB	denotes	expressed
T1793	2939-2941	IN	denotes	in
T1794	2942-2947	JJ	denotes	naive
T1795	2948-2954	NN	denotes	CD4+ T
T1796	2955-2960	NN	denotes	cells
T1797	2960-2961	-COMMA-	denotes	,
T1798	2962-2965	PRP-DOLLAR-	denotes	its
T1799	2966-2976	NN	denotes	expression
T1800	2977-2979	VB	denotes	is
T1801	2980-2992	VB	denotes	up-regulated
T1802	2993-2999	IN	denotes	during
T1803	3000-3003	NN	denotes	Th1
T1804	3004-3008	NN	denotes	cell
T1805	3009-3024	NN	denotes	differentiation
T1806	3024-3025	-COMMA-	denotes	,
T1807	3026-3029	CC	denotes	and
T1808	3030-3035	NN	denotes	Runx3
T1809	3036-3046	VB	denotes	influences
T1810	3047-3050	NN	denotes	Th1
T1811	3051-3055	NN	denotes	cell
T1812	3056-3071	NN	denotes	differentiation
T1813	3072-3075	CC	denotes	and
T1814	3076-3084	NN	denotes	function
T1815	3085-3092	IN	denotes	through
T1816	3093-3099	JJ	denotes	direct
T1817	3100-3110	NN	denotes	regulation
T1818	3111-3113	IN	denotes	of
T1819	3114-3139	NN	denotes	the Il4 and Ifng cytokine
T1820	3140-3145	NN	denotes	genes
T1821	3146-3147	-LRB-	denotes	(
T1822	3147-3153	CD	denotes	15, 16
T1823	3153-3154	-RRB-	denotes	)
T1824	3156-3158	IN	denotes	In
T1825	3159-3167	NN	denotes	contrast
T1826	3167-3168	-COMMA-	denotes	,
T1827	3169-3172	DT	denotes	all
T1828	3173-3178	CD	denotes	three
T1829	3179-3183	NN	denotes	Runx
T1830	3184-3192	NN	denotes	proteins
T1831	3193-3196	VB	denotes	are
T1832	3197-3206	VB	denotes	expressed
T1833	3207-3209	IN	denotes	in
T1834	3210-3216	JJ	denotes	mature
T1835	3217-3223	NN	denotes	CD8+ T
T1836	3224-3229	NN	denotes	cells
T1837	3230-3231	-LRB-	denotes	(
T1838	3231-3237	CD	denotes	10, 12
T1839	3237-3238	-RRB-	denotes	)
T1840	3238-3239	-COMMA-	denotes	,
T1841	3240-3243	CC	denotes	and
T1842	3244-3259	JJ	denotes	Runx3-deficient
T1843	3260-3266	NN	denotes	CD8+ T
T1844	3267-3272	NN	denotes	cells
T1845	3273-3277	VB	denotes	show
T1846	3278-3285	VB	denotes	reduced
T1847	3286-3295	JJ	denotes	cytolytic
T1848	3296-3304	NN	denotes	activity
T1849	3305-3306	-LRB-	denotes	(
T1850	3306-3312	CD	denotes	12, 13
T1851	3312-3313	-RRB-	denotes	)
T1852	3315-3317	PRP	denotes	We
T1853	3318-3327	RB	denotes	therefore
T1854	3328-3334	VB	denotes	tested
T1855	3335-3342	IN	denotes	whether
T1856	3343-3348	NN	denotes	Runx3
T1857	3349-3359	VB	denotes	influenced
T1858	3360-3369	JJ	denotes	cytolytic
T1859	3370-3371	NN	denotes	T
T1860	3372-3376	NN	denotes	cell
T1861	3377-3396	NN	denotes	differentiation. In
T1862	3397-3401	DT	denotes	this
T1863	3402-3408	NN	denotes	report
T1864	3408-3409	-COMMA-	denotes	,
T1865	3410-3412	PRP	denotes	we
T1866	3413-3417	VB	denotes	show
T1867	3418-3422	IN	denotes	that
T1868	3423-3428	NN	denotes	Runx3
T1869	3429-3432	CC	denotes	and
T1870	3433-3438	NN	denotes	T-box
T1871	3439-3446	NN	denotes	factors
T1872	3447-3462	RB	denotes	synergistically
T1873	3463-3471	VB	denotes	regulate
T1874	3472-3475	NN	denotes	CTL
T1875	3476-3491	NN	denotes	differentiation
T1876	3492-3495	CC	denotes	and
T1877	3496-3504	NN	denotes	function
T1878	3506-3511	NN	denotes	T-bet
T1879	3512-3514	VB	denotes	is
T1880	3515-3522	VB	denotes	induced
T1881	3523-3530	RB	denotes	quickly
T1882	3531-3535	IN	denotes	upon
T1883	3536-3539	NN	denotes	TCR
T1884	3540-3551	NN	denotes	stimulation
T1885	3552-3555	CC	denotes	and
T1886	3556-3558	VB	denotes	is
T1887	3559-3567	VB	denotes	required
T1888	3568-3571	IN	denotes	for
T1889	3572-3577	JJ	denotes	early
T1890	3578-3589	NN	denotes	programming
T1891	3590-3592	IN	denotes	of
T1892	3593-3601	NN	denotes	cytokine
T1893	3602-3612	NN	denotes	production
T1894	3613-3614	-LRB-	denotes	(
T1895	3614-3616	CD	denotes	17
T1896	3616-3617	-RRB-	denotes	)
T1897	3617-3618	-COMMA-	denotes	,
T1898	3619-3626	IN	denotes	whereas
T1899	3627-3632	NN	denotes	Eomes
T1900	3633-3635	VB	denotes	is
T1901	3636-3643	VB	denotes	induced
T1902	3644-3649	RB	denotes	later
T1903	3650-3656	IN	denotes	during
T1904	3657-3672	NN	denotes	differentiation
T1905	3673-3676	CC	denotes	and
T1906	3677-3685	VB	denotes	sustains
T1907	3686-3691	NN	denotes	IFN-γ
T1908	3692-3702	NN	denotes	expression
T1909	3704-3709	NN	denotes	Runx3
T1910	3710-3712	VB	denotes	is
T1911	3713-3721	VB	denotes	required
T1912	3722-3725	IN	denotes	for
T1913	3726-3731	NN	denotes	Eomes
T1914	3732-3735	CC	denotes	and
T1915	3736-3744	NN	denotes	perforin
T1916	3745-3755	NN	denotes	expression
T1917	3755-3756	-COMMA-	denotes	,
T1918	3757-3760	CC	denotes	and
T1919	3761-3765	DT	denotes	both
T1920	3766-3771	NN	denotes	Eomes
T1921	3772-3775	CC	denotes	and
T1922	3776-3781	NN	denotes	Runx3
T1923	3782-3786	VB	denotes	bind
T1924	3787-3789	IN	denotes	at
T1925	3790-3804	NN	denotes	the Prf1 locus
T1926	3804-3805	-COLON-	denotes	;
T1927	3806-3808	IN	denotes	in
T1928	3809-3817	NN	denotes	contrast
T1929	3817-3818	-COMMA-	denotes	,
T1930	3819-3827	NN	denotes	perforin
T1931	3828-3838	NN	denotes	expression
T1932	3839-3841	VB	denotes	is
T1933	3842-3852	JJ	denotes	unaffected
T1934	3853-3855	IN	denotes	in
T1935	3856-3871	JJ	denotes	T-bet–deficient
T1936	3872-3877	NN	denotes	cells
T1937	3879-3880	NN	denotes	T
T1938	3881-3886	NN	denotes	cells
T1939	3887-3894	VB	denotes	lacking
T1940	3895-3900	NN	denotes	Runx3
T1941	3901-3905	NN	denotes	show
T1942	3906-3915	VB	denotes	decreased
T1943	3916-3926	NN	denotes	expression
T1944	3927-3929	IN	denotes	of
T1945	3930-3935	NN	denotes	IFN-γ
T1946	3936-3939	CC	denotes	and
T1947	3940-3948	NN	denotes	granzyme
T1948	3949-3950	NN	denotes	B
T1949	3950-3951	-COMMA-	denotes	,
T1950	3952-3955	CC	denotes	and
T1951	3956-3961	NN	denotes	Runx3
T1952	3962-3966	RB	denotes	also
T1953	3967-3972	VB	denotes	binds
T1954	3973-3976	DT	denotes	the
T1955	3977-3985	NN	denotes	promoter
T1956	3986-3993	NN	denotes	regions
T1957	3994-3996	IN	denotes	of
T1958	3997-4020	NN	denotes	the Ifng and Gzmb genes
T1959	4022-4034	RB	denotes	Collectively
T1960	4034-4035	-COMMA-	denotes	,
T1961	4036-4041	DT	denotes	these
T1962	4042-4049	NN	denotes	results
T1963	4050-4057	VB	denotes	provide
T1964	4058-4066	NN	denotes	evidence
T1965	4067-4070	IN	denotes	for
T1966	4071-4072	DT	denotes	a
T1967	4073-4080	JJ	denotes	complex
T1968	4081-4096	JJ	denotes	transcriptional
T1969	4097-4104	NN	denotes	network
T1970	4105-4107	IN	denotes	in
T1971	4108-4113	WDT	denotes	which
T1972	4114-4119	NN	denotes	Runx3
T1973	4120-4122	VB	denotes	is
T1974	4123-4124	DT	denotes	a
T1975	4125-4132	JJ	denotes	primary
T1976	4133-4142	NN	denotes	regulator
T1977	4143-4161	NN	denotes	of Gzmb expression
T1978	4162-4165	CC	denotes	but
T1979	4166-4176	VB	denotes	synergizes
T1980	4177-4181	IN	denotes	with
T1981	4182-4187	NN	denotes	T-bet
T1982	4188-4191	CC	denotes	and
T1983	4192-4197	NN	denotes	Eomes
T1984	4197-4198	-COMMA-	denotes	,
T1985	4199-4211	RB	denotes	respectively
T1986	4211-4212	-COMMA-	denotes	,
T1987	4213-4215	TO	denotes	to
T1988	4216-4223	VB	denotes	promote
T1989	4224-4237	NN	denotes	transcription
T1990	4238-4240	IN	denotes	of
T1991	4241-4264	NN	denotes	the Ifng and Prf1 genes
T2913	4291-4292	DT	denotes	A
T2914	4293-4297	NN	denotes	cell
T2915	4298-4305	NN	denotes	culture
T2916	4306-4312	NN	denotes	system
T2917	4313-4315	TO	denotes	to
T2918	4316-4323	VB	denotes	monitor
T2919	4324-4332	NN	denotes	effector
T2920	4333-4336	NN	denotes	CTL
T2921	4337-4352	NN	denotes	differentiation
T2922	4353-4355	PRP	denotes	We
T2923	4356-4360	VB	denotes	used
T2924	4361-4362	DT	denotes	a
T2925	4363-4369	JJ	denotes	simple
T2926	4370-4374	NN	denotes	cell
T2927	4375-4382	NN	denotes	culture
T2928	4383-4389	NN	denotes	system
T2929	4390-4392	TO	denotes	to
T2930	4393-4400	VB	denotes	examine
T2931	4401-4404	DT	denotes	the
T2932	4405-4413	NN	denotes	kinetics
T2933	4414-4416	IN	denotes	of
T2934	4417-4425	NN	denotes	effector
T2935	4426-4430	NN	denotes	gene
T2936	4431-4441	NN	denotes	expression
T2937	4442-4448	IN	denotes	during
T2938	4449-4453	JJ	denotes	CD8+
T2939	4454-4455	NN	denotes	T
T2940	4456-4460	NN	denotes	cell
T2941	4461-4476	NN	denotes	differentiation
T2942	4478-4483	JJ	denotes	Naive
T2943	4484-4488	JJ	denotes	CD8+
T2944	4489-4490	NN	denotes	T
T2945	4491-4496	NN	denotes	cells
T2946	4497-4501	IN	denotes	from
T2947	4502-4505	NN	denotes	P14
T2948	4506-4509	NN	denotes	TCR
T2949	4510-4520	JJ	denotes	transgenic
T2950	4521-4525	NN	denotes	mice
T2951	4526-4530	VB	denotes	were
T2952	4531-4540	VB	denotes	activated
T2953	4541-4544	IN	denotes	for
T2954	4545-4546	CD	denotes	2
T2955	4547-4548	NN	denotes	d
T2956	4549-4553	IN	denotes	with
T2957	4554-4562	NN	denotes	anti-CD3
T2958	4563-4566	CC	denotes	and
T2959	4567-4576	NN	denotes	anti-CD28
T2960	4577-4579	CC	denotes	or
T2961	4580-4584	IN	denotes	with
T2962	4585-4592	JJ	denotes	splenic
T2963	4593-4597	NN	denotes	APCs
T2964	4598-4600	IN	denotes	in
T2965	4601-4604	DT	denotes	the
T2966	4605-4613	NN	denotes	presence
T2967	4614-4616	IN	denotes	of
T2968	4617-4621	NN	denotes	Gp33
T2969	4622-4629	NN	denotes	peptide
T2970	4629-4630	-COMMA-	denotes	,
T2971	4631-4634	CC	denotes	and
T2972	4635-4639	VB	denotes	were
T2973	4640-4648	VB	denotes	cultured
T2974	4649-4651	IN	denotes	in
T2975	4652-4657	NN	denotes	media
T2976	4658-4668	VB	denotes	containing
T2977	4669-4672	CD	denotes	100
T2978	4673-4677	NN	denotes	U/ml
T2979	4678-4680	IN	denotes	of
T2980	4681-4692	JJ	denotes	recombinant
T2981	4693-4698	JJ	denotes	human
T2982	4699-4703	NN	denotes	IL-2
T2983	4704-4705	-LRB-	denotes	(
T2984	4705-4711	NN	denotes	rhIL-2
T2985	4711-4712	-RRB-	denotes	)
T2986	4714-4716	PRP	denotes	We
T2987	4717-4721	VB	denotes	used
T2988	4722-4725	NN	denotes	TCR
T2989	4726-4736	JJ	denotes	transgenic
T2990	4737-4741	NN	denotes	mice
T2991	4742-4745	IN	denotes	for
T2992	4746-4751	DT	denotes	these
T2993	4752-4763	NN	denotes	experiments
T2994	4764-4771	IN	denotes	because
T2995	4772-4776	PRP	denotes	they
T2996	4777-4784	VB	denotes	provide
T2997	4785-4786	DT	denotes	a
T2998	4787-4795	JJ	denotes	reliable
T2999	4796-4802	NN	denotes	source
T3000	4803-4805	IN	denotes	of
T3001	4806-4810	JJ	denotes	CD8+
T3002	4811-4812	NN	denotes	T
T3003	4813-4818	NN	denotes	cells
T3004	4819-4823	WDT	denotes	that
T3005	4824-4827	VB	denotes	are
T3006	4828-4833	RB	denotes	truly
T3007	4834-4839	JJ	denotes	naive
T3008	4839-4840	-COLON-	denotes	;
T3009	4841-4848	RB	denotes	however
T3010	4848-4849	-COMMA-	denotes	,
T3011	4850-4852	PRP	denotes	we
T3012	4853-4858	VB	denotes	chose
T3013	4859-4862	RB	denotes	not
T3014	4863-4865	TO	denotes	to
T3015	4866-4875	VB	denotes	stimulate
T3016	4876-4881	NN	denotes	cells
T3017	4882-4886	IN	denotes	with
T3018	4887-4894	NN	denotes	antigen
T3019	4895-4897	IN	denotes	in
T3020	4898-4902	JJ	denotes	most
T3021	4903-4914	NN	denotes	experiments
T3022	4915-4917	RB	denotes	so
T3023	4918-4920	IN	denotes	as
T3024	4921-4923	TO	denotes	to
T3025	4924-4929	VB	denotes	avoid
T3026	4930-4943	NN	denotes	contamination
T3027	4944-4948	IN	denotes	with
T3028	4949-4957	NN	denotes	proteins
T3029	4958-4961	CC	denotes	and
T3030	4962-4969	JJ	denotes	nucleic
T3031	4970-4975	NN	denotes	acids
T3032	4976-4983	VB	denotes	derived
T3033	4984-4988	IN	denotes	from
T3034	4989-4993	NN	denotes	APCs
T3035	4995-5000	EX	denotes	There
T3036	5001-5005	VB	denotes	were
T3037	5006-5010	RB	denotes	only
T3038	5011-5016	JJ	denotes	minor
T3039	5017-5028	NN	denotes	differences
T3040	5029-5031	IN	denotes	in
T3041	5032-5036	NN	denotes	gene
T3042	5037-5047	NN	denotes	expression
T3043	5048-5054	IN	denotes	during
T3044	5055-5070	NN	denotes	differentiation
T3045	5071-5078	VB	denotes	induced
T3046	5079-5081	IN	denotes	by
T3047	5082-5093	NN	denotes	antigen/APC
T3048	5094-5100	IN	denotes	versus
T3049	5101-5119	JJ	denotes	anti-CD3/anti-CD28
T3050	5119-5120	-COMMA-	denotes	,
T3051	5121-5124	CC	denotes	and
T3052	5125-5128	DT	denotes	the
T3053	5129-5134	JJ	denotes	major
T3054	5135-5146	NN	denotes	conclusions
T3055	5147-5156	VB	denotes	presented
T3056	5157-5159	IN	denotes	in
T3057	5160-5164	DT	denotes	this
T3058	5165-5171	NN	denotes	report
T3059	5172-5175	VB	denotes	are
T3060	5176-5179	DT	denotes	the
T3061	5180-5184	JJ	denotes	same
T3062	5185-5188	IN	denotes	for
T3063	5189-5193	DT	denotes	both
T3064	5194-5204	VB	denotes	activating
T3065	5205-5215	NN	denotes	conditions
T3066	5217-5222	IN	denotes	Under
T3067	5223-5226	PRP-DOLLAR-	denotes	our
T3068	5227-5234	NN	denotes	culture
T3069	5235-5245	NN	denotes	conditions
T3070	5245-5246	-COMMA-	denotes	,
T3071	5247-5256	VB	denotes	activated
T3072	5257-5261	JJ	denotes	CD8+
T3073	5262-5263	NN	denotes	T
T3074	5264-5269	NN	denotes	cells
T3075	5270-5278	VB	denotes	expanded
T3076	5279-5292	RB	denotes	exponentially
T3077	5293-5296	CC	denotes	and
T3078	5297-5308	VB	denotes	accumulated
T3079	5309-5312	IN	denotes	for
T3080	5313-5314	JJ	denotes	>
T3081	5314-5315	CD	denotes	8
T3082	5316-5317	NN	denotes	d
T3083	5319-5321	PRP	denotes	We
T3084	5322-5329	VB	denotes	limited
T3085	5330-5333	PRP-DOLLAR-	denotes	our
T3086	5334-5342	NN	denotes	analysis
T3087	5343-5345	TO	denotes	to
T3088	5346-5349	DT	denotes	the
T3089	5350-5355	JJ	denotes	first
T3090	5356-5359	CD	denotes	6–8
T3091	5360-5361	NN	denotes	d
T3092	5362-5367	IN	denotes	after
T3093	5368-5378	NN	denotes	activation
T3094	5378-5379	-COMMA-	denotes	,
T3095	5380-5381	DT	denotes	a
T3096	5382-5388	NN	denotes	period
T3097	5389-5393	WDT	denotes	that
T3098	5394-5403	VB	denotes	coincides
T3099	5404-5408	IN	denotes	with
T3100	5409-5415	JJ	denotes	clonal
T3101	5416-5425	NN	denotes	expansion
T3102	5426-5428	IN	denotes	of
T3103	5429-5433	JJ	denotes	CD8+
T3104	5434-5435	NN	denotes	T
T3105	5436-5441	NN	denotes	cells
T3106	5442-5447	IN	denotes	after
T3107	5448-5458	NN	denotes	activation
T3108	5459-5461	FW	denotes	in
T3109	5462-5466	FW	denotes	vivo
T3825	5469-5477	JJ	denotes	Distinct
T3826	5478-5488	NN	denotes	expression
T3827	5489-5497	NN	denotes	kinetics
T3828	5498-5500	IN	denotes	of
T3829	5501-5509	NN	denotes	perforin
T3830	5510-5513	CC	denotes	and
T3831	5514-5522	NN	denotes	granzyme
T3832	5523-5524	NN	denotes	B
T3833	5525-5531	IN	denotes	during
T3834	5532-5535	NN	denotes	CTL
T3835	5536-5547	NN	denotes	development
T3836	5548-5550	IN	denotes	in
T3837	5551-5558	NN	denotes	culture
T3838	5559-5562	PRP-DOLLAR-	denotes	Our
T3839	5563-5574	NN	denotes	experiments
T3840	5575-5583	VB	denotes	revealed
T3841	5584-5589	JJ	denotes	clear
T3842	5590-5601	NN	denotes	differences
T3843	5602-5604	IN	denotes	in
T3844	5605-5608	DT	denotes	the
T3845	5609-5617	NN	denotes	kinetics
T3846	5618-5620	IN	denotes	of
T3847	5621-5629	NN	denotes	perforin
T3848	5629-5630	-COMMA-	denotes	,
T3849	5631-5639	NN	denotes	granzyme
T3850	5640-5641	NN	denotes	B
T3851	5641-5642	-COMMA-	denotes	,
T3852	5643-5646	CC	denotes	and
T3853	5647-5655	NN	denotes	cytokine
T3854	5656-5666	NN	denotes	expression
T3855	5667-5673	IN	denotes	during
T3856	5674-5678	JJ	denotes	CD8+
T3857	5679-5680	NN	denotes	T
T3858	5681-5685	NN	denotes	cell
T3859	5686-5696	NN	denotes	activation
T3860	5697-5698	-LRB-	denotes	(
T3861	5698-5702	NN	denotes	Fig.
T3862	5703-5704	CD	denotes	1
T3863	5704-5705	-RRB-	denotes	)
T3864	5707-5712	JJ	denotes	Naive
T3865	5713-5714	NN	denotes	T
T3866	5715-5720	NN	denotes	cells
T3867	5721-5727	VB	denotes	showed
T3868	5728-5738	JJ	denotes	detectable
T3869	5739-5749	NN	denotes	expression
T3870	5750-5752	IN	denotes	of
T3871	5753-5761	NN	denotes	perforin
T3872	5762-5766	NN	denotes	mRNA
T3873	5767-5769	RB	denotes	as
T3874	5770-5774	RB	denotes	well
T3875	5775-5777	IN	denotes	as
T3876	5778-5786	NN	denotes	perforin
T3877	5787-5794	NN	denotes	protein
T3878	5795-5796	-LRB-	denotes	(
T3879	5796-5800	NN	denotes	Fig.
T3880	5801-5802	CD	denotes	1
T3881	5802-5803	-COMMA-	denotes	,
T3882	5804-5807	NN	denotes	A–D
T3883	5807-5808	-RRB-	denotes	)
T3884	5810-5818	JJ	denotes	Relative
T3885	5819-5821	TO	denotes	to
T3886	5822-5825	PRP-DOLLAR-	denotes	its
T3887	5826-5836	NN	denotes	expression
T3888	5837-5839	IN	denotes	in
T3889	5840-5845	JJ	denotes	naive
T3890	5846-5847	NN	denotes	T
T3891	5848-5853	NN	denotes	cells
T3892	5853-5854	-COMMA-	denotes	,
T3893	5855-5863	NN	denotes	perforin
T3894	5864-5865	-LRB-	denotes	(
T3895	5865-5869	NN	denotes	Prf1
T3896	5869-5870	-RRB-	denotes	)
T3897	5871-5875	NN	denotes	mRNA
T3898	5876-5886	NN	denotes	expression
T3899	5887-5890	VB	denotes	did
T3900	5891-5894	RB	denotes	not
T3901	5895-5903	VB	denotes	increase
T3902	5904-5915	RB	denotes	appreciably
T3903	5916-5918	IN	denotes	at
T3904	5919-5922	NN	denotes	day
T3905	5923-5924	CD	denotes	2
T3906	5925-5928	CC	denotes	but
T3907	5929-5935	VB	denotes	showed
T3908	5936-5937	DT	denotes	a
T3909	5938-5950	JJ	denotes	reproducible
T3910	5951-5959	NN	denotes	decrease
T3911	5960-5962	IN	denotes	at
T3912	5963-5966	NN	denotes	day
T3913	5967-5968	CD	denotes	4
T3914	5968-5969	-COMMA-	denotes	,
T3915	5970-5978	VB	denotes	followed
T3916	5979-5981	IN	denotes	by
T3917	5982-5988	JJ	denotes	robust
T3918	5989-6001	NN	denotes	reexpression
T3919	6002-6009	IN	denotes	between
T3920	6010-6014	NN	denotes	days
T3921	6015-6016	CD	denotes	4
T3922	6017-6020	CC	denotes	and
T3923	6021-6022	CD	denotes	8
T3924	6023-6024	-LRB-	denotes	(
T3925	6024-6028	NN	denotes	Fig.
T3926	6029-6030	CD	denotes	1
T3927	6030-6031	-COMMA-	denotes	,
T3928	6032-6035	NN	denotes	A–D
T3929	6035-6036	-RRB-	denotes	)
T3930	6038-6040	IN	denotes	In
T3931	6041-6049	NN	denotes	contrast
T3932	6049-6050	-COMMA-	denotes	,
T3933	6051-6059	NN	denotes	granzyme
T3934	6060-6061	NN	denotes	B
T3935	6062-6063	-LRB-	denotes	(
T3936	6063-6067	NN	denotes	Gzmb
T3937	6067-6068	-RRB-	denotes	)
T3938	6069-6073	NN	denotes	mRNA
T3939	6074-6077	VB	denotes	was
T3940	6078-6081	JJ	denotes	low
T3941	6082-6084	CC	denotes	or
T3942	6085-6097	JJ	denotes	undetectable
T3943	6098-6100	IN	denotes	in
T3944	6101-6106	JJ	denotes	naive
T3945	6107-6108	NN	denotes	T
T3946	6109-6114	NN	denotes	cells
T3947	6115-6118	CC	denotes	but
T3948	6119-6122	VB	denotes	was
T3949	6123-6131	RB	denotes	strongly
T3950	6132-6144	VB	denotes	up-regulated
T3951	6145-6147	IN	denotes	by
T3952	6148-6151	NN	denotes	day
T3953	6152-6153	CD	denotes	2
T3954	6154-6159	IN	denotes	after
T3955	6160-6171	NN	denotes	stimulation
T3956	6172-6175	CC	denotes	and
T3957	6176-6185	VB	denotes	increased
T3958	6186-6199	RB	denotes	progressively
T3959	6200-6205	IN	denotes	until
T3960	6206-6209	NN	denotes	day
T3961	6210-6211	CD	denotes	6
T3962	6212-6213	-LRB-	denotes	(
T3963	6213-6217	NN	denotes	Fig.
T3964	6218-6219	CD	denotes	1
T3965	6219-6220	-COMMA-	denotes	,
T3966	6221-6222	NN	denotes	A
T3967	6223-6226	CC	denotes	and
T3968	6227-6228	NN	denotes	B
T3969	6228-6229	-RRB-	denotes	)
T3970	6229-6230	-COLON-	denotes	;
T3971	6231-6240	RB	denotes	similarly
T3972	6240-6241	-COMMA-	denotes	,
T3973	6242-6250	NN	denotes	granzyme
T3974	6251-6252	NN	denotes	B
T3975	6253-6260	NN	denotes	protein
T3976	6261-6264	VB	denotes	was
T3977	6265-6274	VB	denotes	expressed
T3978	6275-6277	IN	denotes	by
T3979	6278-6281	NN	denotes	day
T3980	6282-6283	CD	denotes	4
T3981	6284-6287	CC	denotes	and
T3982	6288-6296	VB	denotes	remained
T3983	6297-6301	JJ	denotes	high
T3984	6302-6307	IN	denotes	until
T3985	6308-6311	NN	denotes	day
T3986	6312-6313	CD	denotes	6
T3987	6314-6315	-LRB-	denotes	(
T3988	6315-6319	NN	denotes	Fig.
T3989	6320-6321	CD	denotes	1
T3990	6322-6323	NN	denotes	E
T3991	6323-6324	-RRB-	denotes	)
T3992	6326-6328	IN	denotes	As
T3993	6329-6337	VB	denotes	expected
T3994	6337-6338	-COMMA-	denotes	,
T3995	6339-6340	DT	denotes	a
T3996	6341-6346	JJ	denotes	small
T3997	6347-6355	NN	denotes	fraction
T3998	6356-6358	IN	denotes	of
T3999	6359-6364	JJ	denotes	naive
T4000	6365-6366	NN	denotes	T
T4001	6367-6372	NN	denotes	cells
T4002	6373-6382	VB	denotes	expressed
T4003	6383-6386	DT	denotes	the
T4004	6387-6396	NN	denotes	cytokines
T4005	6397-6402	NN	denotes	IFN-γ
T4006	6403-6406	CC	denotes	and
T4007	6407-6410	NN	denotes	TNF
T4008	6411-6413	IN	denotes	in
T4009	6414-6422	NN	denotes	response
T4010	6423-6425	TO	denotes	to
T4011	6426-6437	NN	denotes	stimulation
T4012	6437-6438	-COMMA-	denotes	,
T4013	6439-6442	CC	denotes	and
T4014	6443-6447	DT	denotes	this
T4015	6448-6456	NN	denotes	capacity
T4016	6457-6466	VB	denotes	increased
T4017	6467-6480	RB	denotes	significantly
T4018	6481-6483	IN	denotes	in
T4019	6484-6498	VB	denotes	differentiated
T4020	6499-6504	NN	denotes	cells
T4021	6505-6506	-LRB-	denotes	(
T4022	6506-6510	NN	denotes	Fig.
T4023	6511-6512	CD	denotes	1
T4024	6513-6514	NN	denotes	E
T4025	6514-6515	-COLON-	denotes	;
T4026	6516-6519	VB	denotes	see
T4027	6520-6524	RB	denotes	also
T4028	6525-6529	NNP	denotes	Fig.
T4029	6530-6531	CD	denotes	2
T4030	6532-6533	NN	denotes	A
T4031	6533-6534	-RRB-	denotes	)
T18624	6546-6554	NN	denotes	Kinetics
T18625	6555-6557	IN	denotes	of
T18626	6558-6562	NN	denotes	gene
T18627	6563-6573	NN	denotes	expression
T18628	6574-6580	IN	denotes	during
T18629	6581-6585	JJ	denotes	CD8+
T18630	6586-6587	NN	denotes	T
T18631	6588-6592	NN	denotes	cell
T18632	6593-6609	NN	denotes	differentiation.
T18633	6610-6611	-LRB-	denotes	(
T18634	6611-6612	NN	denotes	A
T18635	6612-6613	-RRB-	denotes	)
T18636	6614-6622	NN	denotes	Kinetics
T18637	6623-6625	IN	denotes	of
T18638	6626-6630	NN	denotes	Prf1
T18639	6630-6631	-COMMA-	denotes	,
T18640	6632-6636	NN	denotes	Gzmb
T18641	6636-6637	-COMMA-	denotes	,
T18642	6638-6643	NN	denotes	Tbx21
T18643	6644-6645	-LRB-	denotes	(
T18644	6645-6650	NN	denotes	T-bet
T18645	6650-6651	-RRB-	denotes	)
T18646	6651-6652	-COMMA-	denotes	,
T18647	6653-6656	CC	denotes	and
T18648	6657-6662	NN	denotes	Eomes
T18649	6663-6667	NN	denotes	mRNA
T18650	6668-6678	NN	denotes	expression
T18651	6679-6681	IN	denotes	in
T18652	6682-6697	VB	denotes	differentiating
T18653	6698-6701	NN	denotes	P14
T18654	6702-6706	JJ	denotes	CD8+
T18655	6707-6708	NN	denotes	T
T18656	6709-6714	NN	denotes	cells
T18657	6715-6723	VB	denotes	analyzed
T18658	6724-6726	IN	denotes	by
T18659	6727-6735	JJ	denotes	Northern
T18660	6736-6744	NN	denotes	blotting
T18661	6746-6749	NN	denotes	RNA
T18662	6750-6754	IN	denotes	from
T18663	6755-6758	NN	denotes	day
T18664	6759-6760	CD	denotes	7
T18665	6761-6764	NN	denotes	Th1
T18666	6765-6770	NN	denotes	cells
T18667	6771-6774	VB	denotes	was
T18668	6775-6779	VB	denotes	used
T18669	6780-6782	IN	denotes	as
T18670	6783-6784	DT	denotes	a
T18671	6785-6792	NN	denotes	control
T18672	6794-6799	NN	denotes	Sizes
T18673	6800-6802	IN	denotes	of
T18674	6803-6807	NN	denotes	mRNA
T18675	6808-6819	NN	denotes	transcripts
T18676	6820-6823	VB	denotes	are
T18677	6824-6834	VB	denotes	indicated.
T18678	6835-6836	-LRB-	denotes	(
T18679	6836-6837	NN	denotes	B
T18680	6837-6838	-RRB-	denotes	)
T18681	6839-6853	NN	denotes	Quantification
T18682	6854-6856	IN	denotes	of
T18683	6857-6865	JJ	denotes	relative
T18684	6866-6870	NN	denotes	mRNA
T18685	6871-6878	NN	denotes	amounts
T18686	6879-6881	IN	denotes	by
T18687	6882-6896	NN	denotes	phosphorimager
T18688	6897-6906	NN	denotes	analysis.
T18689	6907-6908	-LRB-	denotes	(
T18690	6908-6909	NN	denotes	C
T18691	6909-6910	-RRB-	denotes	)
T18692	6911-6919	NN	denotes	Kinetics
T18693	6920-6922	IN	denotes	of
T18694	6923-6930	NN	denotes	protein
T18695	6931-6941	NN	denotes	expression
T18696	6942-6944	IN	denotes	in
T18697	6945-6960	VB	denotes	differentiating
T18698	6961-6964	NN	denotes	P14
T18699	6965-6969	JJ	denotes	CD8+
T18700	6970-6971	NN	denotes	T
T18701	6972-6977	NN	denotes	cells
T18702	6978-6986	VB	denotes	analyzed
T18703	6987-6989	IN	denotes	by
T18704	6990-7004	NN	denotes	immunoblotting
T18705	7006-7011	NN	denotes	Sizes
T18706	7012-7014	IN	denotes	of
T18707	7015-7022	NN	denotes	protein
T18708	7023-7028	NN	denotes	bands
T18709	7029-7032	VB	denotes	are
T18710	7033-7043	VB	denotes	indicated.
T18711	7044-7045	-LRB-	denotes	(
T18712	7045-7046	NN	denotes	D
T18713	7046-7047	-RRB-	denotes	)
T18714	7048-7056	JJ	denotes	Relative
T18715	7057-7064	NN	denotes	protein
T18716	7065-7072	NN	denotes	amounts
T18717	7073-7083	VB	denotes	quantified
T18718	7084-7088	IN	denotes	from
T18719	7089-7092	DT	denotes	the
T18720	7093-7100	JJ	denotes	Western
T18721	7101-7107	NN	denotes	blots.
T18722	7108-7109	-LRB-	denotes	(
T18723	7109-7110	NN	denotes	E
T18724	7110-7111	-RRB-	denotes	)
T18725	7112-7125	JJ	denotes	Intracellular
T18726	7126-7134	NN	denotes	staining
T18727	7135-7138	IN	denotes	for
T18728	7139-7147	NN	denotes	granzyme
T18729	7148-7149	NN	denotes	B
T18730	7149-7150	-COMMA-	denotes	,
T18731	7151-7156	NN	denotes	IFN-γ
T18732	7156-7157	-COMMA-	denotes	,
T18733	7158-7161	CC	denotes	and
T18734	7162-7165	NN	denotes	TNF
T18735	7167-7175	NN	denotes	Granzyme
T18736	7176-7177	NN	denotes	B
T18737	7178-7186	NN	denotes	staining
T18738	7187-7190	VB	denotes	was
T18739	7191-7199	JJ	denotes	specific
T18740	7200-7208	JJ	denotes	relative
T18741	7209-7211	TO	denotes	to
T18742	7212-7214	DT	denotes	an
T18743	7215-7222	NN	denotes	isotype
T18744	7223-7230	NN	denotes	control
T18745	7231-7232	-LRB-	denotes	(
T18746	7232-7235	RB	denotes	not
T18747	7236-7244	VB	denotes	depicted
T18748	7244-7245	-RRB-	denotes	)
T18749	7247-7252	NN	denotes	Cells
T18750	7253-7257	VB	denotes	were
T18751	7258-7270	VB	denotes	restimulated
T18752	7271-7275	IN	denotes	with
T18753	7276-7279	NN	denotes	PMA
T18754	7280-7283	CC	denotes	and
T18755	7284-7293	NN	denotes	ionomycin
T18756	7294-7297	IN	denotes	for
T18757	7298-7299	CD	denotes	4
T18758	7300-7302	NN	denotes	h.
T18759	7303-7304	-LRB-	denotes	(
T18760	7304-7305	NN	denotes	F
T18761	7305-7306	-RRB-	denotes	)
T18762	7307-7317	JJ	denotes	FACS-based
T18763	7318-7323	NN	denotes	assay
T18764	7324-7326	TO	denotes	to
T18765	7327-7334	VB	denotes	measure
T18766	7335-7344	JJ	denotes	cytolytic
T18767	7345-7353	NN	denotes	activity
T18768	7354-7356	IN	denotes	of
T18769	7357-7360	NN	denotes	P14
T18770	7361-7365	JJ	denotes	CD8+
T18771	7366-7367	NN	denotes	T
T18772	7368-7373	NN	denotes	cells
T18773	7374-7381	IN	denotes	against
T18774	7382-7385	NN	denotes	EL4
T18775	7386-7393	NN	denotes	targets
T18776	7394-7400	VB	denotes	loaded
T18777	7401-7405	IN	denotes	with
T18778	7406-7407	CD	denotes	0
T18779	7408-7409	-LRB-	denotes	(
T18780	7409-7410	JJ\|RB	denotes	−
T18781	7410-7411	-RRB-	denotes	)
T18782	7412-7414	CC	denotes	or
T18783	7415-7416	CD	denotes	1
T18784	7417-7418	-LRB-	denotes	(
T18785	7418-7419	SYM	denotes	+
T18786	7419-7420	-RRB-	denotes	)
T18787	7421-7423	NN	denotes	μM
T18788	7424-7428	NN	denotes	Gp33
T18789	7429-7436	NN	denotes	peptide
T18790	7437-7438	-LRB-	denotes	(
T18791	7438-7456	JJ	denotes	effector-to-target
T18792	7457-7462	NN	denotes	ratio
T18793	7463-7464	SYM	denotes	=
T18794	7465-7466	CD	denotes	5
T18795	7466-7467	SYM	denotes	:
T18796	7467-7468	CD	denotes	1
T18797	7468-7469	-RRB-	denotes	)
T18798	7471-7481	NN	denotes	Percentage
T18799	7482-7484	IN	denotes	of
T18800	7485-7492	NN	denotes	Annexin
T18801	7493-7495	JJ	denotes	V+
T18802	7496-7497	-LRB-	denotes	(
T18803	7497-7506	JJ	denotes	apoptotic
T18804	7506-7507	-RRB-	denotes	)
T18805	7508-7514	NN	denotes	target
T18806	7515-7520	NN	denotes	cells
T18807	7521-7523	IN	denotes	in
T18808	7524-7527	DT	denotes	the
T18809	7528-7540	JJ	denotes	CD8-negative
T18810	7541-7544	NN	denotes	EL4
T18811	7545-7551	NN	denotes	target
T18812	7552-7562	NN	denotes	population
T18813	7563-7564	-LRB-	denotes	(
T18814	7564-7567	NN	denotes	dot
T18815	7568-7573	NN	denotes	plots
T18816	7573-7574	-RRB-	denotes	)
T18817	7575-7578	VB	denotes	was
T18818	7579-7589	VB	denotes	determined
T18819	7590-7591	-LRB-	denotes	(
T18820	7591-7601	NN	denotes	histograms
T18821	7601-7602	-RRB-	denotes	)
T18822	7604-7613	JJ	denotes	Cytolytic
T18823	7614-7622	NN	denotes	activity
T18824	7623-7626	VB	denotes	was
T18825	7627-7634	VB	denotes	blocked
T18826	7635-7637	IN	denotes	by
T18827	7638-7648	NN	denotes	incubation
T18828	7649-7653	IN	denotes	with
T18829	7654-7655	CD	denotes	2
T18830	7656-7658	NN	denotes	mM
T18831	7659-7663	NN	denotes	EGTA
T18832	7664-7665	-LRB-	denotes	(
T18833	7665-7668	RB	denotes	not
T18834	7669-7677	VB	denotes	depicted
T18835	7677-7678	-RRB-	denotes	)
T18836	7678-7679	-COMMA-	denotes	,
T18837	7680-7690	VB	denotes	confirming
T18838	7691-7702	NN	denotes	involvement
T18839	7703-7705	IN	denotes	of
T18840	7706-7709	DT	denotes	the
T18841	7710-7717	NN	denotes	granule
T18842	7718-7728	NN	denotes	exocytosis
T18843	7729-7730	-LRB-	denotes	(
T18844	7730-7747	NN	denotes	perforin–granzyme
T18845	7748-7749	NN	denotes	B
T18846	7749-7750	-RRB-	denotes	)
T18847	7751-7758	NN	denotes	pathway
T18848	7760-7764	NN	denotes	Data
T18849	7765-7768	VB	denotes	are
T18850	7769-7783	JJ	denotes	representative
T18851	7784-7786	IN	denotes	of
T18852	7787-7789	IN	denotes	at
T18853	7790-7795	JJ	denotes	least
T18854	7796-7800	CD	denotes	five
T18855	7801-7802	-LRB-	denotes	(
T18856	7802-7805	NN	denotes	A–E
T18857	7805-7806	-RRB-	denotes	)
T18858	7807-7809	CC	denotes	or
T18859	7810-7815	CD	denotes	three
T18860	7816-7817	-LRB-	denotes	(
T18861	7817-7818	NN	denotes	F
T18862	7818-7819	-RRB-	denotes	)
T18863	7820-7831	JJ	denotes	independent
T18864	7832-7843	NN	denotes	experiments
T4032	7844-7846	PRP	denotes	We
T4033	7847-7856	VB	denotes	evaluated
T4034	7857-7874	JJ	denotes	antigen-dependent
T4035	7875-7884	JJ	denotes	cytolytic
T4036	7885-7893	NN	denotes	function
T4037	7894-7896	IN	denotes	in
T4038	7897-7898	DT	denotes	a
T4039	7899-7909	JJ	denotes	short-term
T4040	7910-7915	NN	denotes	assay
T4041	7916-7918	IN	denotes	in
T4042	7919-7924	WDT	denotes	which
T4043	7925-7931	NN	denotes	target
T4044	7932-7936	NN	denotes	cell
T4045	7937-7942	NN	denotes	death
T4046	7943-7946	VB	denotes	was
T4047	7947-7955	VB	denotes	measured
T4048	7956-7962	IN	denotes	within
T4049	7963-7964	CD	denotes	2
T4050	7965-7966	NN	denotes	h
T4051	7967-7968	-LRB-	denotes	(
T4052	7968-7972	NN	denotes	Fig.
T4053	7973-7974	CD	denotes	1
T4054	7975-7976	NN	denotes	F
T4055	7976-7977	-RRB-	denotes	)
T4056	7979-7981	IN	denotes	By
T4057	7982-7990	VB	denotes	limiting
T4058	7991-7994	DT	denotes	the
T4059	7995-8003	NN	denotes	duration
T4060	8004-8006	IN	denotes	of
T4061	8007-8010	NN	denotes	TCR
T4062	8011-8022	NN	denotes	stimulation
T4063	8022-8023	-COMMA-	denotes	,
T4064	8024-8028	DT	denotes	this
T4065	8029-8037	NN	denotes	strategy
T4066	8038-8047	VB	denotes	minimizes
T4067	8048-8057	NN	denotes	cytolysis
T4068	8058-8067	JJ	denotes	secondary
T4069	8068-8070	TO	denotes	to
T4070	8071-8074	JJ	denotes	new
T4071	8075-8079	NN	denotes	gene
T4072	8080-8090	NN	denotes	expression
T4073	8091-8097	IN	denotes	during
T4074	8098-8101	DT	denotes	the
T4075	8102-8108	NN	denotes	period
T4076	8109-8111	IN	denotes	of
T4077	8112-8115	DT	denotes	the
T4078	8116-8121	NN	denotes	assay
T4079	8123-8128	JJ	denotes	Naive
T4080	8129-8130	NN	denotes	T
T4081	8131-8136	NN	denotes	cells
T4082	8137-8140	VB	denotes	did
T4083	8141-8144	RB	denotes	not
T4084	8145-8152	VB	denotes	display
T4085	8153-8164	JJ	denotes	significant
T4086	8165-8174	JJ	denotes	cytolytic
T4087	8175-8183	NN	denotes	function
T4088	8184-8186	IN	denotes	in
T4089	8187-8191	DT	denotes	this
T4090	8192-8202	JJ	denotes	short-term
T4091	8203-8208	NN	denotes	assay
T4092	8209-8210	-LRB-	denotes	(
T4093	8210-8221	JJ	denotes	unpublished
T4094	8222-8226	NN	denotes	data
T4095	8226-8227	-RRB-	denotes	)
T4096	8227-8228	-COMMA-	denotes	,
T4097	8229-8233	RB	denotes	most
T4098	8234-8240	RB	denotes	likely
T4099	8241-8248	IN	denotes	because
T4100	8249-8253	PRP	denotes	they
T4101	8254-8261	VB	denotes	express
T4102	8262-8270	JJ	denotes	immature
T4103	8271-8272	-LRB-	denotes	(
T4104	8272-8283	JJ	denotes	unprocessed
T4105	8283-8284	-RRB-	denotes	)
T4106	8285-8290	NN	denotes	forms
T4107	8291-8293	IN	denotes	of
T4108	8294-8302	NN	denotes	perforin
T4109	8303-8306	CC	denotes	and
T4110	8307-8311	VB	denotes	lack
T4111	8312-8315	DT	denotes	the
T4112	8316-8324	NN	denotes	capacity
T4113	8325-8327	TO	denotes	to
T4114	8328-8339	VB	denotes	degranulate
T4115	8340-8341	-LRB-	denotes	(
T4116	8341-8343	CD	denotes	18
T4117	8343-8344	-COMMA-	denotes	,
T4118	8345-8347	CD	denotes	19
T4119	8347-8348	-RRB-	denotes	)
T4120	8350-8354	RB	denotes	Even
T4121	8355-8360	IN	denotes	after
T4122	8361-8371	NN	denotes	activation
T4123	8372-8375	IN	denotes	for
T4124	8376-8377	CD	denotes	2
T4125	8378-8380	CC	denotes	or
T4126	8381-8382	CD	denotes	4
T4127	8383-8384	NN	denotes	d
T4128	8384-8385	-COMMA-	denotes	,
T4129	8386-8389	DT	denotes	the
T4130	8390-8395	NN	denotes	cells
T4131	8396-8402	VB	denotes	showed
T4132	8403-8407	JJ	denotes	poor
T4133	8408-8417	JJ	denotes	cytolytic
T4134	8418-8426	NN	denotes	activity
T4135	8427-8428	-LRB-	denotes	(
T4136	8428-8432	NN	denotes	Fig.
T4137	8433-8434	CD	denotes	1
T4138	8435-8436	NN	denotes	F
T4139	8436-8437	-RRB-	denotes	)
T4140	8437-8438	-COMMA-	denotes	,
T4141	8439-8441	IN	denotes	in
T4142	8442-8450	JJ	denotes	striking
T4143	8451-8459	NN	denotes	contrast
T4144	8460-8462	TO	denotes	to
T4145	8463-8468	PRP-DOLLAR-	denotes	their
T4146	8469-8477	NN	denotes	capacity
T4147	8478-8481	IN	denotes	for
T4148	8482-8491	JJ	denotes	efficient
T4149	8492-8500	NN	denotes	cytokine
T4150	8501-8511	NN	denotes	production
T4151	8512-8513	-LRB-	denotes	(
T4152	8513-8517	NN	denotes	Fig.
T4153	8518-8519	CD	denotes	1
T4154	8520-8521	NN	denotes	E
T4155	8521-8522	-RRB-	denotes	)
T4156	8524-8528	JJ	denotes	Only
T4157	8529-8534	NN	denotes	cells
T4158	8535-8543	VB	denotes	cultured
T4159	8544-8549	IN	denotes	until
T4160	8550-8553	NN	denotes	day
T4161	8554-8555	CD	denotes	6
T4162	8556-8565	VB	denotes	displayed
T4163	8566-8572	JJ	denotes	robust
T4164	8573-8585	NN	denotes	cytotoxicity
T4165	8585-8586	-COMMA-	denotes	,
T4166	8587-8589	IN	denotes	as
T4167	8590-8596	VB	denotes	judged
T4168	8597-8599	IN	denotes	by
T4169	8600-8605	PRP-DOLLAR-	denotes	their
T4170	8606-8613	NN	denotes	ability
T4171	8614-8616	TO	denotes	to
T4172	8617-8623	VB	denotes	induce
T4173	8624-8633	NN	denotes	apoptosis
T4174	8634-8636	IN	denotes	in
T4175	8637-8638	DT	denotes	a
T4176	8639-8644	JJ	denotes	large
T4177	8645-8651	NN	denotes	number
T4178	8652-8654	IN	denotes	of
T4179	8655-8661	NN	denotes	target
T4180	8662-8667	NN	denotes	cells
T4181	8668-8669	-LRB-	denotes	(
T4182	8669-8673	NN	denotes	Fig.
T4183	8674-8675	CD	denotes	1
T4184	8676-8677	NN	denotes	F
T4185	8677-8678	-RRB-	denotes	)
T4186	8680-8685	DT	denotes	These
T4187	8686-8693	NN	denotes	results
T4188	8694-8698	VB	denotes	show
T4189	8699-8703	IN	denotes	that
T4190	8704-8709	IN	denotes	after
T4191	8710-8711	DT	denotes	a
T4192	8712-8718	JJ	denotes	strong
T4193	8719-8726	NN	denotes	priming
T4194	8727-8735	NN	denotes	stimulus
T4195	8736-8743	IN	denotes	through
T4196	8744-8748	NN	denotes	TCRs
T4197	8749-8752	CC	denotes	and
T4198	8753-8767	JJ	denotes	co-stimulatory
T4199	8768-8777	NN	denotes	receptors
T4200	8778-8780	FW	denotes	in
T4201	8781-8786	FW	denotes	vitro
T4202	8786-8787	-COMMA-	denotes	,
T4203	8788-8796	NN	denotes	granzyme
T4204	8797-8798	NN	denotes	B
T4205	8799-8809	NN	denotes	expression
T4206	8810-8813	CC	denotes	and
T4207	8814-8817	DT	denotes	the
T4208	8818-8825	NN	denotes	ability
T4209	8826-8828	TO	denotes	to
T4210	8829-8836	VB	denotes	produce
T4211	8837-8845	NN	denotes	effector
T4212	8846-8855	NN	denotes	cytokines
T4213	8856-8859	VB	denotes	are
T4214	8860-8870	VB	denotes	programmed
T4215	8871-8876	RB	denotes	early
T4216	8876-8877	-COMMA-	denotes	,
T4217	8878-8885	IN	denotes	whereas
T4218	8886-8894	NN	denotes	perforin
T4219	8895-8905	NN	denotes	expression
T4220	8906-8909	CC	denotes	and
T4221	8910-8919	JJ	denotes	cytolytic
T4222	8920-8928	NN	denotes	function
T4223	8929-8932	VB	denotes	are
T4224	8933-8940	VB	denotes	induced
T4225	8941-8946	RB	denotes	later
T4226	8946-8947	-COMMA-	denotes	,
T4227	8948-8954	IN	denotes	during
T4228	8955-8958	DT	denotes	the
T4229	8959-8964	NN	denotes	phase
T4230	8965-8967	IN	denotes	of
T4231	8968-8974	JJ	denotes	clonal
T4232	8975-8984	NN	denotes	expansion
T4233	8985-8987	IN	denotes	in
T4234	8988-8992	NN	denotes	IL-2
T4235	8994-9003	RB	denotes	Therefore
T4236	9003-9004	-COMMA-	denotes	,
T4237	9005-9008	DT	denotes	the
T4238	9009-9012	CD	denotes	two
T4239	9013-9018	JJ	denotes	major
T4240	9019-9027	NN	denotes	effector
T4241	9028-9037	NN	denotes	functions
T4242	9038-9040	IN	denotes	of
T4243	9041-9044	NN	denotes	CTL
T4244	9044-9045	-COMMA-	denotes	,
T4245	9046-9054	NN	denotes	cytokine
T4246	9055-9065	NN	denotes	production
T4247	9066-9069	CC	denotes	and
T4248	9070-9079	JJ	denotes	cytolytic
T4249	9080-9088	NN	denotes	activity
T4250	9088-9089	-COMMA-	denotes	,
T4251	9090-9093	VB	denotes	are
T4252	9094-9097	RB	denotes	not
T4253	9098-9111	RB	denotes	intrinsically
T4254	9112-9123	VB	denotes	coregulated
T5120	9126-9134	JJ	denotes	Distinct
T5121	9135-9143	NN	denotes	kinetics
T5122	9144-9146	IN	denotes	of
T5123	9147-9152	NN	denotes	T-bet
T5124	9153-9156	CC	denotes	and
T5125	9157-9162	NN	denotes	Eomes
T5126	9163-9173	NN	denotes	expression
T5127	9174-9180	IN	denotes	during
T5128	9181-9184	NN	denotes	CTL
T5129	9185-9200	NN	denotes	differentiation
T5130	9201-9204	DT	denotes	The
T5131	9205-9210	NN	denotes	T-box
T5132	9211-9224	NN	denotes	transcription
T5133	9225-9232	NN	denotes	factors
T5134	9233-9238	NN	denotes	T-bet
T5135	9239-9242	CC	denotes	and
T5136	9243-9248	NN	denotes	Eomes
T5137	9249-9253	VB	denotes	have
T5138	9254-9258	VB	denotes	been
T5139	9259-9265	VB	denotes	linked
T5140	9266-9268	TO	denotes	to
T5141	9269-9272	DT	denotes	the
T5142	9273-9283	NN	denotes	regulation
T5143	9284-9286	IN	denotes	of
T5144	9287-9292	NN	denotes	genes
T5145	9293-9301	VB	denotes	encoding
T5146	9302-9310	NN	denotes	effector
T5147	9311-9320	NN	denotes	cytokines
T5148	9321-9322	-LRB-	denotes	(
T5149	9322-9326	FW	denotes	e.g.
T5150	9326-9327	-COMMA-	denotes	,
T5151	9328-9332	NN	denotes	Ifng
T5152	9332-9333	-RRB-	denotes	)
T5153	9334-9337	CC	denotes	and
T5154	9338-9343	NN	denotes	genes
T5155	9344-9353	JJ	denotes	important
T5156	9354-9357	IN	denotes	for
T5157	9358-9367	JJ	denotes	cytolytic
T5158	9368-9376	NN	denotes	function
T5159	9377-9378	-LRB-	denotes	(
T5160	9378-9382	FW	denotes	e.g.
T5161	9382-9383	-COMMA-	denotes	,
T5162	9384-9388	NN	denotes	Prf1
T5163	9389-9392	CC	denotes	and
T5164	9393-9397	NN	denotes	GzmB
T5165	9397-9398	-RRB-	denotes	)
T5166	9399-9400	-LRB-	denotes	(
T5167	9400-9402	CD	denotes	20
T5168	9402-9403	-RRB-	denotes	)
T5169	9405-9407	PRP	denotes	We
T5170	9408-9420	VB	denotes	investigated
T5171	9421-9424	DT	denotes	the
T5172	9425-9433	NN	denotes	kinetics
T5173	9434-9436	IN	denotes	of
T5174	9437-9447	NN	denotes	expression
T5175	9448-9450	IN	denotes	of
T5176	9451-9456	DT	denotes	these
T5177	9457-9470	NN	denotes	transcription
T5178	9471-9478	NN	denotes	factors
T5179	9479-9481	IN	denotes	in
T5180	9482-9485	PRP-DOLLAR-	denotes	our
T5181	9486-9488	FW	denotes	in
T5182	9489-9494	FW	denotes	vitro
T5183	9495-9503	NN	denotes	cultures
T5184	9504-9505	-LRB-	denotes	(
T5185	9505-9509	NNP	denotes	Fig.
T5186	9510-9511	CD	denotes	1
T5187	9511-9512	-COMMA-	denotes	,
T5188	9513-9516	NN	denotes	A–D
T5189	9516-9517	-RRB-	denotes	)
T5190	9519-9524	NN	denotes	T-bet
T5191	9525-9529	NN	denotes	mRNA
T5192	9530-9533	CC	denotes	and
T5193	9534-9541	NN	denotes	protein
T5194	9542-9546	VB	denotes	were
T5195	9547-9550	RB	denotes	not
T5196	9551-9561	JJ	denotes	detectable
T5197	9562-9564	IN	denotes	in
T5198	9565-9570	JJ	denotes	naive
T5199	9571-9575	JJ	denotes	CD8+
T5200	9576-9577	NN	denotes	T
T5201	9578-9583	NN	denotes	cells
T5202	9583-9584	-COMMA-	denotes	,
T5203	9585-9588	CC	denotes	but
T5204	9589-9593	VB	denotes	were
T5205	9594-9602	RB	denotes	strongly
T5206	9603-9610	VB	denotes	induced
T5207	9611-9615	IN	denotes	upon
T5208	9616-9619	NN	denotes	TCR
T5209	9620-9627	NN	denotes	priming
T5210	9628-9629	-LRB-	denotes	(
T5211	9629-9632	NN	denotes	day
T5212	9633-9634	CD	denotes	2
T5213	9634-9635	-RRB-	denotes	)
T5214	9636-9639	CC	denotes	and
T5215	9640-9648	VB	denotes	remained
T5216	9649-9658	VB	denotes	expressed
T5217	9659-9666	IN	denotes	through
T5218	9667-9670	NN	denotes	day
T5219	9671-9672	CD	denotes	6
T5220	9673-9675	IN	denotes	of
T5221	9676-9691	NN	denotes	differentiation
T5222	9692-9693	-LRB-	denotes	(
T5223	9693-9697	NN	denotes	Fig.
T5224	9698-9699	CD	denotes	1
T5225	9699-9700	-COMMA-	denotes	,
T5226	9701-9702	NN	denotes	A
T5227	9703-9706	CC	denotes	and
T5228	9707-9708	NN	denotes	C
T5229	9708-9709	-COLON-	denotes	;
T5230	9710-9720	VB	denotes	quantified
T5231	9721-9723	IN	denotes	in
T5232	9724-9728	NNP	denotes	Fig.
T5233	9729-9730	CD	denotes	1
T5234	9730-9731	-COMMA-	denotes	,
T5235	9732-9733	NN	denotes	B
T5236	9734-9737	CC	denotes	and
T5237	9738-9739	NN	denotes	D
T5238	9739-9740	-RRB-	denotes	)
T5239	9742-9744	IN	denotes	In
T5240	9745-9753	NN	denotes	contrast
T5241	9753-9754	-COMMA-	denotes	,
T5242	9755-9760	NN	denotes	Eomes
T5243	9761-9771	NN	denotes	expression
T5244	9772-9775	VB	denotes	was
T5245	9776-9779	JJ	denotes	low
T5246	9780-9782	CC	denotes	or
T5247	9783-9795	JJ	denotes	undetectable
T5248	9796-9798	IN	denotes	at
T5249	9799-9803	CC	denotes	both
T5250	9804-9807	DT	denotes	the
T5251	9808-9812	NN	denotes	mRNA
T5252	9813-9816	CC	denotes	and
T5253	9817-9824	NN	denotes	protein
T5254	9825-9831	NN	denotes	levels
T5255	9832-9834	IN	denotes	in
T5256	9835-9840	JJ	denotes	naive
T5257	9841-9845	JJ	denotes	CD8+
T5258	9846-9847	NN	denotes	T
T5259	9848-9853	NN	denotes	cells
T5260	9853-9854	-COMMA-	denotes	,
T5261	9855-9858	CC	denotes	and
T5262	9859-9862	NN	denotes	TCR
T5263	9863-9870	NN	denotes	priming
T5264	9871-9873	IN	denotes	in
T5265	9874-9881	NN	denotes	culture
T5266	9882-9885	VB	denotes	had
T5267	9886-9890	RB	denotes	only
T5268	9891-9892	DT	denotes	a
T5269	9893-9899	JJ	denotes	modest
T5270	9900-9906	NN	denotes	effect
T5271	9907-9909	IN	denotes	on
T5272	9910-9913	PRP-DOLLAR-	denotes	its
T5273	9914-9924	NN	denotes	expression
T5274	9925-9927	IN	denotes	at
T5275	9928-9931	NN	denotes	day
T5276	9932-9933	CD	denotes	2
T5277	9934-9935	-LRB-	denotes	(
T5278	9935-9939	NN	denotes	Fig.
T5279	9940-9941	CD	denotes	1
T5280	9941-9942	-COMMA-	denotes	,
T5281	9943-9944	NN	denotes	A
T5282	9945-9948	CC	denotes	and
T5283	9949-9950	NN	denotes	C
T5284	9950-9951	-RRB-	denotes	)
T5285	9953-9959	JJ	denotes	Strong
T5286	9960-9969	NN	denotes	induction
T5287	9970-9972	IN	denotes	of
T5288	9973-9978	NN	denotes	Eomes
T5289	9979-9983	NN	denotes	mRNA
T5290	9984-9987	CC	denotes	and
T5291	9988-9995	NN	denotes	protein
T5292	9996-9999	VB	denotes	was
T5293	10000-10004	RB	denotes	only
T5294	10005-10013	VB	denotes	observed
T5295	10014-10016	IN	denotes	at
T5296	10017-10020	NN	denotes	day
T5297	10021-10022	CD	denotes	4
T5298	10023-10026	CC	denotes	and
T5299	10027-10032	RB	denotes	later
T5300	10033-10034	-LRB-	denotes	(
T5301	10034-10038	NNP	denotes	Fig.
T5302	10039-10040	CD	denotes	1
T5303	10040-10041	-COMMA-	denotes	,
T5304	10042-10043	NN	denotes	A
T5305	10044-10047	CC	denotes	and
T5306	10048-10049	NN	denotes	C
T5307	10049-10050	-RRB-	denotes	)
T5308	10052-10057	NN	denotes	T-bet
T5309	10058-10062	NN	denotes	mRNA
T5310	10063-10073	NN	denotes	expression
T5311	10074-10082	RB	denotes	slightly
T5312	10083-10091	VB	denotes	preceded
T5313	10092-10095	DT	denotes	the
T5314	10096-10106	NN	denotes	expression
T5315	10107-10109	IN	denotes	of
T5316	10110-10114	NN	denotes	GzmB
T5317	10115-10119	NN	denotes	mRNA
T5318	10119-10120	-COLON-	denotes	;
T5319	10121-10130	RB	denotes	similarly
T5320	10130-10131	-COMMA-	denotes	,
T5321	10132-10137	NN	denotes	Eomes
T5322	10138-10142	NN	denotes	mRNA
T5323	10143-10146	CC	denotes	and
T5324	10147-10154	NN	denotes	protein
T5325	10155-10159	VB	denotes	were
T5326	10160-10169	VB	denotes	expressed
T5327	10170-10172	CD	denotes	∼1
T5328	10173-10174	NN	denotes	d
T5329	10175-10180	RB	denotes	ahead
T5330	10181-10183	IN	denotes	of
T5331	10184-10187	DT	denotes	the
T5332	10188-10200	NN	denotes	reexpression
T5333	10201-10203	IN	denotes	of
T5334	10204-10212	NN	denotes	perforin
T5335	10213-10217	NN	denotes	mRNA
T5336	10218-10221	CC	denotes	and
T5337	10222-10229	NN	denotes	protein
T5338	10229-10230	-COMMA-	denotes	,
T5339	10231-10243	RB	denotes	respectively
T5340	10244-10245	-LRB-	denotes	(
T5341	10245-10249	NNP	denotes	Fig.
T5342	10250-10251	CD	denotes	1
T5343	10251-10252	-COMMA-	denotes	,
T5344	10253-10254	NN	denotes	B
T5345	10255-10258	CC	denotes	and
T5346	10259-10260	NN	denotes	D
T5347	10260-10261	-RRB-	denotes	)
T5348	10263-10267	DT	denotes	This
T5349	10268-10276	JJ	denotes	detailed
T5350	10277-10284	JJ	denotes	kinetic
T5351	10285-10293	NN	denotes	analysis
T5352	10294-10303	VB	denotes	suggested
T5353	10304-10308	IN	denotes	that
T5354	10308-10309	-COMMA-	denotes	,
T5355	10310-10315	IN	denotes	under
T5356	10316-10319	PRP-DOLLAR-	denotes	our
T5357	10320-10327	NN	denotes	culture
T5358	10328-10338	NN	denotes	conditions
T5359	10338-10339	-COMMA-	denotes	,
T5360	10340-10345	NN	denotes	T-bet
T5361	10346-10349	CC	denotes	and
T5362	10350-10355	NN	denotes	Eomes
T5363	10356-10366	VB	denotes	contribute
T5364	10367-10369	TO	denotes	to
T5365	10370-10378	JJ	denotes	distinct
T5366	10379-10386	NN	denotes	aspects
T5367	10387-10389	IN	denotes	of
T5368	10390-10394	NN	denotes	gene
T5369	10395-10408	NN	denotes	transcription
T5370	10409-10415	IN	denotes	during
T5371	10416-10419	NN	denotes	CTL
T5372	10420-10435	NN	denotes	differentiation
T5373	10437-10442	NN	denotes	T-bet
T5374	10443-10445	VB	denotes	is
T5375	10446-10454	VB	denotes	required
T5376	10455-10460	RB	denotes	early
T5377	10461-10464	IN	denotes	for
T5378	10465-10470	NN	denotes	IFN-γ
T5379	10471-10481	NN	denotes	production
T5380	10481-10482	-COMMA-	denotes	,
T5381	10483-10486	CC	denotes	and
T5382	10487-10490	PRP-DOLLAR-	denotes	our
T5383	10491-10495	NN	denotes	data
T5384	10496-10505	VB	denotes	suggested
T5385	10506-10510	IN	denotes	that
T5386	10511-10516	NN	denotes	Eomes
T5387	10517-10522	MD	denotes	might
T5388	10523-10526	RB	denotes	not
T5389	10527-10535	VB	denotes	function
T5390	10536-10542	IN	denotes	during
T5391	10543-10547	DT	denotes	this
T5392	10548-10553	JJ	denotes	early
T5393	10554-10560	NN	denotes	period
T5394	10561-10564	CC	denotes	but
T5395	10565-10571	RB	denotes	rather
T5396	10572-10577	MD	denotes	might
T5397	10578-10588	VB	denotes	contribute
T5398	10589-10594	RB	denotes	later
T5399	10595-10597	TO	denotes	to
T5400	10598-10601	DT	denotes	the
T5401	10602-10609	NN	denotes	control
T5402	10610-10612	IN	denotes	of
T5403	10613-10621	NN	denotes	perforin
T5404	10622-10632	NN	denotes	expression
T5405	10634-10637	PRP-DOLLAR-	denotes	Our
T5406	10638-10642	NN	denotes	data
T5407	10643-10649	VB	denotes	seemed
T5408	10650-10654	RB	denotes	most
T5409	10655-10665	JJ	denotes	consistent
T5410	10666-10670	IN	denotes	with
T5411	10671-10672	DT	denotes	a
T5412	10673-10678	NN	denotes	model
T5413	10679-10681	IN	denotes	in
T5414	10682-10687	WDT	denotes	which
T5415	10688-10691	NN	denotes	TCR
T5416	10692-10699	NN	denotes	signals
T5417	10700-10706	VB	denotes	induce
T5418	10707-10712	NN	denotes	T-bet
T5419	10712-10713	-COMMA-	denotes	,
T5420	10714-10719	WDT	denotes	which
T5421	10720-10722	IN	denotes	in
T5422	10723-10727	NN	denotes	turn
T5423	10728-10735	VB	denotes	induces
T5424	10736-10741	NN	denotes	IFN-γ
T5425	10742-10743	-LRB-	denotes	(
T5426	10743-10745	CD	denotes	17
T5427	10745-10746	-RRB-	denotes	)
T5428	10747-10750	CC	denotes	and
T5429	10751-10759	RB	denotes	possibly
T5430	10760-10768	NN	denotes	granzyme
T5431	10769-10770	NN	denotes	B
T5432	10770-10771	-COLON-	denotes	;
T5433	10772-10784	RB	denotes	subsequently
T5434	10784-10785	-COMMA-	denotes	,
T5435	10786-10791	NN	denotes	Eomes
T5436	10792-10794	VB	denotes	is
T5437	10795-10802	VB	denotes	induced
T5438	10803-10809	IN	denotes	during
T5439	10810-10813	DT	denotes	the
T5440	10814-10820	NN	denotes	period
T5441	10821-10823	IN	denotes	of
T5442	10824-10830	JJ	denotes	clonal
T5443	10831-10840	NN	denotes	expansion
T5444	10841-10843	IN	denotes	in
T5445	10844-10848	NN	denotes	IL-2
T5446	10849-10852	CC	denotes	and
T5447	10853-10862	VB	denotes	activates
T5448	10863-10871	NN	denotes	perforin
T5449	10872-10882	NN	denotes	expression
T6493	10885-10893	NN	denotes	Perforin
T6494	10894-10897	CC	denotes	and
T6495	10898-10906	NN	denotes	granzyme
T6496	10907-10908	NN	denotes	B
T6497	10909-10919	NN	denotes	expression
T6498	10920-10923	VB	denotes	are
T6499	10924-10927	RB	denotes	not
T6500	10928-10939	RB	denotes	appreciably
T6501	10940-10949	VB	denotes	regulated
T6502	10950-10952	IN	denotes	by
T6503	10953-10958	NN	denotes	T-bet
T6504	10959-10961	TO	denotes	To
T6505	10962-10966	VB	denotes	test
T6506	10967-10970	DT	denotes	the
T6507	10971-10976	NN	denotes	model
T6508	10977-10985	VB	denotes	outlined
T6509	10986-10988	IN	denotes	in
T6510	10989-10992	DT	denotes	the
T6511	10993-11001	JJ	denotes	previous
T6512	11002-11011	NN	denotes	paragraph
T6513	11012-11020	RB	denotes	directly
T6514	11020-11021	-COMMA-	denotes	,
T6515	11022-11024	PRP	denotes	we
T6516	11025-11033	VB	denotes	compared
T6517	11034-11037	DT	denotes	the
T6518	11038-11048	NN	denotes	expression
T6519	11049-11051	IN	denotes	of
T6520	11052-11057	NN	denotes	IFN-γ
T6521	11057-11058	-COMMA-	denotes	,
T6522	11059-11067	NN	denotes	perforin
T6523	11067-11068	-COMMA-	denotes	,
T6524	11069-11072	CC	denotes	and
T6525	11073-11081	NN	denotes	granzyme
T6526	11082-11083	NN	denotes	B
T6527	11084-11086	IN	denotes	in
T6528	11087-11091	JJ	denotes	CD8+
T6529	11092-11093	NN	denotes	T
T6530	11094-11099	NN	denotes	cells
T6531	11100-11104	IN	denotes	from
T6532	11105-11107	NN	denotes	WT
T6533	11108-11111	CC	denotes	and
T6534	11112-11117	NN	denotes	Tbx21
T6535	11118-11119	-LRB-	denotes	(
T6536	11119-11124	NN	denotes	T-bet
T6537	11124-11125	-RRB-	denotes	)
T6538	11125-11135	JJ	denotes	-deficient
T6539	11136-11140	NN	denotes	mice
T6540	11142-11144	IN	denotes	As
T6541	11145-11153	VB	denotes	expected
T6542	11154-11155	-LRB-	denotes	(
T6543	11155-11157	CD	denotes	17
T6544	11157-11158	-COMMA-	denotes	,
T6545	11159-11161	CD	denotes	21
T6546	11161-11162	-RRB-	denotes	)
T6547	11162-11163	-COMMA-	denotes	,
T6548	11164-11169	JJ	denotes	naive
T6549	11170-11178	NN	denotes	Tbx21−/−
T6550	11179-11183	JJ	denotes	CD8+
T6551	11184-11185	NN	denotes	T
T6552	11186-11191	NN	denotes	cells
T6553	11192-11200	VB	denotes	produced
T6554	11201-11206	NN	denotes	IFN-γ
T6555	11207-11213	RB	denotes	poorly
T6556	11214-11218	IN	denotes	upon
T6557	11219-11229	NN	denotes	activation
T6558	11230-11231	-LRB-	denotes	(
T6559	11231-11235	NN	denotes	Fig.
T6560	11236-11237	CD	denotes	2
T6561	11238-11239	NN	denotes	A
T6562	11239-11240	-RRB-	denotes	)
T6563	11242-11249	RB	denotes	Notably
T6564	11249-11250	-COMMA-	denotes	,
T6565	11251-11255	DT	denotes	this
T6566	11256-11267	JJ	denotes	deleterious
T6567	11268-11274	NN	denotes	effect
T6568	11275-11277	IN	denotes	of
T6569	11278-11283	NN	denotes	T-bet
T6570	11284-11294	NN	denotes	deficiency
T6571	11295-11298	VB	denotes	was
T6572	11299-11303	RB	denotes	only
T6573	11304-11312	VB	denotes	observed
T6574	11313-11315	IN	denotes	in
T6575	11316-11331	VB	denotes	differentiating
T6576	11332-11336	JJ	denotes	CD8+
T6577	11337-11338	NN	denotes	T
T6578	11339-11344	NN	denotes	cells
T6579	11345-11350	IN	denotes	until
T6580	11351-11354	NN	denotes	day
T6581	11355-11356	CD	denotes	4
T6582	11357-11359	IN	denotes	of
T6583	11360-11367	NN	denotes	culture
T6584	11368-11371	CC	denotes	but
T6585	11372-11375	VB	denotes	was
T6586	11376-11382	RB	denotes	almost
T6587	11383-11393	RB	denotes	completely
T6588	11394-11403	VB	denotes	mitigated
T6589	11404-11406	IN	denotes	by
T6590	11407-11410	NN	denotes	day
T6591	11411-11412	CD	denotes	6
T6592	11413-11414	-LRB-	denotes	(
T6593	11414-11418	NN	denotes	Fig.
T6594	11419-11420	CD	denotes	2
T6595	11421-11422	NN	denotes	A
T6596	11422-11423	-RRB-	denotes	)
T6597	11425-11429	DT	denotes	This
T6598	11430-11434	RB	denotes	most
T6599	11435-11441	JJ	denotes	likely
T6600	11442-11451	VB	denotes	reflected
T6601	11452-11464	NN	denotes	compensation
T6602	11465-11467	IN	denotes	by
T6603	11468-11473	NN	denotes	Eomes
T6604	11473-11474	-COMMA-	denotes	,
T6605	11475-11480	WDT	denotes	which
T6606	11481-11484	VB	denotes	was
T6607	11485-11493	RB	denotes	strongly
T6608	11494-11501	VB	denotes	induced
T6609	11502-11509	IN	denotes	between
T6610	11510-11514	NN	denotes	days
T6611	11515-11516	CD	denotes	4
T6612	11517-11520	CC	denotes	and
T6613	11521-11522	CD	denotes	6
T6614	11523-11524	-LRB-	denotes	(
T6615	11524-11528	NN	denotes	Fig.
T6616	11529-11530	CD	denotes	1
T6617	11530-11531	-RRB-	denotes	)
T6618	11533-11535	IN	denotes	In
T6619	11536-11544	NN	denotes	contrast
T6620	11544-11545	-COMMA-	denotes	,
T6621	11546-11561	JJ	denotes	T-bet–deficient
T6622	11562-11563	NN	denotes	T
T6623	11564-11569	NN	denotes	cells
T6624	11570-11578	VB	denotes	cultured
T6625	11579-11582	IN	denotes	for
T6626	11583-11584	CD	denotes	6
T6627	11585-11586	NN	denotes	d
T6628	11587-11593	VB	denotes	showed
T6629	11594-11596	DT	denotes	no
T6630	11597-11603	NN	denotes	defect
T6631	11604-11606	IN	denotes	in
T6632	11607-11615	NN	denotes	perforin
T6633	11616-11620	NN	denotes	mRNA
T6634	11621-11631	NN	denotes	expression
T6635	11632-11633	-LRB-	denotes	(
T6636	11633-11637	NN	denotes	Fig.
T6637	11638-11639	CD	denotes	2
T6638	11640-11641	NN	denotes	B
T6639	11641-11642	-COMMA-	denotes	,
T6640	11643-11650	VB	denotes	compare
T6641	11651-11656	NN	denotes	lanes
T6642	11657-11658	CD	denotes	1
T6643	11659-11662	CC	denotes	and
T6644	11663-11664	CD	denotes	4
T6645	11664-11665	-RRB-	denotes	)
T6646	11667-11669	PRP	denotes	We
T6647	11670-11682	RB	denotes	consistently
T6648	11683-11691	VB	denotes	observed
T6649	11692-11693	DT	denotes	a
T6650	11694-11700	JJ	denotes	modest
T6651	11701-11710	NN	denotes	reduction
T6652	11711-11713	IN	denotes	in
T6653	11714-11718	NN	denotes	GzmB
T6654	11719-11723	NN	denotes	mRNA
T6655	11724-11726	IN	denotes	in
T6656	11727-11742	JJ	denotes	T-bet–deficient
T6657	11743-11744	NN	denotes	T
T6658	11745-11750	NN	denotes	cells
T6659	11751-11752	-LRB-	denotes	(
T6660	11752-11756	NN	denotes	Fig.
T6661	11757-11758	CD	denotes	2
T6662	11759-11760	NN	denotes	B
T6663	11760-11761	-COMMA-	denotes	,
T6664	11762-11769	VB	denotes	compare
T6665	11770-11775	NN	denotes	lanes
T6666	11776-11777	CD	denotes	1
T6667	11778-11781	CC	denotes	and
T6668	11782-11783	CD	denotes	4
T6669	11783-11784	-RRB-	denotes	)
T6670	11784-11785	-COMMA-	denotes	,
T6671	11786-11791	WDT	denotes	which
T6672	11792-11795	VB	denotes	did
T6673	11796-11799	RB	denotes	not
T6674	11800-11809	VB	denotes	translate
T6675	11810-11814	IN	denotes	into
T6676	11815-11816	DT	denotes	a
T6677	11817-11825	NN	denotes	decrease
T6678	11826-11828	IN	denotes	in
T6679	11829-11839	NN	denotes	expression
T6680	11840-11842	IN	denotes	of
T6681	11843-11851	NN	denotes	granzyme
T6682	11852-11853	NN	denotes	B
T6683	11854-11861	NN	denotes	protein
T6684	11862-11863	-LRB-	denotes	(
T6685	11863-11867	NN	denotes	Fig.
T6686	11868-11869	CD	denotes	2
T6687	11870-11871	NN	denotes	C
T6688	11871-11872	-RRB-	denotes	)
T19474	11884-11894	NN	denotes	Regulation
T19475	11895-11897	IN	denotes	of
T19476	11898-11906	NN	denotes	perforin
T19477	11906-11907	-COMMA-	denotes	,
T19478	11908-11916	NN	denotes	granzyme
T19479	11917-11918	NN	denotes	B
T19480	11918-11919	-COMMA-	denotes	,
T19481	11920-11923	CC	denotes	and
T19482	11924-11929	NN	denotes	IFN-γ
T19483	11930-11940	NN	denotes	expression
T19484	11941-11943	IN	denotes	by
T19485	11944-11949	NN	denotes	T-bet
T19486	11950-11953	CC	denotes	and
T19487	11954-11959	NN	denotes	Eomes
T19488	11960-11962	IN	denotes	in
T19489	11963-11978	VB	denotes	differentiating
T19490	11979-11984	NN	denotes	CTLs.
T19491	11985-11986	-LRB-	denotes	(
T19492	11986-11987	NN	denotes	A
T19493	11987-11988	-RRB-	denotes	)
T19494	11989-11994	NN	denotes	IFN-γ
T19495	11995-12005	NN	denotes	expression
T19496	12006-12008	IN	denotes	by
T19497	12009-12011	NN	denotes	WT
T19498	12012-12013	-LRB-	denotes	(
T19499	12013-12021	NN	denotes	Tbx21+/+
T19500	12021-12022	-RRB-	denotes	)
T19501	12023-12026	CC	denotes	and
T19502	12027-12042	JJ	denotes	T-bet–deficient
T19503	12043-12044	-LRB-	denotes	(
T19504	12044-12052	NN	denotes	Tbx21−/−
T19505	12052-12053	-RRB-	denotes	)
T19506	12054-12055	NN	denotes	T
T19507	12056-12061	NN	denotes	cells
T19508	12063-12068	JJ	denotes	Naive
T19509	12069-12073	JJ	denotes	CD8+
T19510	12074-12075	NN	denotes	T
T19511	12076-12081	NN	denotes	cells
T19512	12081-12082	-COMMA-	denotes	,
T19513	12083-12085	CC	denotes	or
T19514	12086-12091	NN	denotes	cells
T19515	12092-12101	VB	denotes	activated
T19516	12102-12105	CC	denotes	and
T19517	12106-12114	VB	denotes	cultured
T19518	12115-12118	IN	denotes	for
T19519	12119-12120	CD	denotes	4
T19520	12121-12123	CC	denotes	or
T19521	12124-12125	CD	denotes	6
T19522	12126-12127	NN	denotes	d
T19523	12127-12128	-COMMA-	denotes	,
T19524	12129-12133	VB	denotes	were
T19525	12134-12146	VB	denotes	restimulated
T19526	12147-12151	IN	denotes	with
T19527	12152-12155	NN	denotes	PMA
T19528	12156-12159	CC	denotes	and
T19529	12160-12169	NN	denotes	ionomycin
T19530	12170-12173	IN	denotes	for
T19531	12174-12175	CD	denotes	6
T19532	12176-12177	NN	denotes	h
T19533	12177-12178	-COMMA-	denotes	,
T19534	12179-12182	CC	denotes	and
T19535	12183-12188	NN	denotes	IFN-γ
T19536	12189-12199	NN	denotes	expression
T19537	12200-12203	VB	denotes	was
T19538	12204-12212	VB	denotes	assessed
T19539	12213-12215	IN	denotes	by
T19540	12216-12229	JJ	denotes	intracellular
T19541	12230-12238	NN	denotes	staining
T19542	12240-12247	NN	denotes	Numbers
T19543	12248-12252	VB	denotes	show
T19544	12253-12256	DT	denotes	the
T19545	12257-12267	NN	denotes	percentage
T19546	12268-12270	IN	denotes	of
T19547	12271-12277	JJ	denotes	IFN-γ+
T19548	12278-12284	NN	denotes	cells.
T19549	12285-12286	-LRB-	denotes	(
T19550	12286-12287	NN	denotes	B
T19551	12287-12288	-RRB-	denotes	)
T19552	12289-12297	JJ	denotes	Northern
T19553	12298-12302	NN	denotes	blot
T19554	12303-12311	NN	denotes	analysis
T19555	12312-12314	IN	denotes	of
T19556	12315-12319	NN	denotes	Prf1
T19557	12320-12323	CC	denotes	and
T19558	12324-12328	NN	denotes	GzmB
T19559	12329-12333	NN	denotes	mRNA
T19560	12334-12344	NN	denotes	expression
T19561	12345-12347	IN	denotes	in
T19562	12348-12350	NN	denotes	WT
T19563	12351-12353	CC	denotes	or
T19564	12354-12369	JJ	denotes	T-bet–deficient
T19565	12370-12374	JJ	denotes	CD8+
T19566	12375-12376	NN	denotes	T
T19567	12377-12382	NN	denotes	cells
T19568	12383-12392	VB	denotes	activated
T19569	12393-12396	CC	denotes	and
T19570	12397-12403	CC	denotes	either
T19571	12404-12408	JJ	denotes	left
T19572	12409-12419	JJ	denotes	uninfected
T19573	12420-12421	-LRB-	denotes	(
T19574	12421-12426	JJ	denotes	uninf
T19575	12426-12427	-RRB-	denotes	)
T19576	12428-12430	CC	denotes	or
T19577	12431-12441	VB	denotes	transduced
T19578	12442-12446	IN	denotes	with
T19579	12447-12459	NN	denotes	retroviruses
T19580	12460-12470	VB	denotes	expressing
T19581	12471-12481	NN	denotes	Eomes-VP16
T19582	12482-12483	-LRB-	denotes	(
T19583	12483-12490	NN	denotes	Eo-VP16
T19584	12490-12491	-RRB-	denotes	)
T19585	12492-12494	CC	denotes	or
T19586	12495-12497	DT	denotes	an
T19587	12498-12503	JJ	denotes	empty
T19588	12504-12512	NN	denotes	IRES-GFP
T19589	12513-12521	NN	denotes	cassette
T19590	12522-12523	-LRB-	denotes	(
T19591	12523-12526	NN	denotes	GFP
T19592	12526-12527	-RRB-	denotes	)
T19593	12529-12534	JJ	denotes	Total
T19594	12535-12543	JJ	denotes	cellular
T19595	12544-12547	NN	denotes	RNA
T19596	12548-12551	VB	denotes	was
T19597	12552-12560	VB	denotes	analyzed
T19598	12561-12563	IN	denotes	on
T19599	12564-12567	NN	denotes	day
T19600	12568-12569	CD	denotes	6
T19601	12570-12572	IN	denotes	of
T19602	12573-12580	NN	denotes	culture
T19603	12582-12585	DT	denotes	The
T19604	12586-12595	NN	denotes	frequency
T19605	12596-12598	IN	denotes	of
T19606	12599-12609	VB	denotes	transduced
T19607	12610-12615	NN	denotes	cells
T19608	12616-12618	IN	denotes	in
T19609	12619-12622	DT	denotes	the
T19610	12623-12631	NN	denotes	cultures
T19611	12632-12635	VB	denotes	was
T19612	12636-12646	JJ	denotes	equivalent
T19613	12647-12650	IN	denotes	for
T19614	12651-12655	DT	denotes	both
T19615	12656-12666	NN	denotes	constructs
T19616	12667-12668	-LRB-	denotes	(
T19617	12668-12674	CD	denotes	∼65–70
T19618	12674-12675	NN	denotes	%
T19619	12676-12680	NN	denotes	GFP+
T19620	12681-12686	NN	denotes	cells
T19621	12686-12687	-COLON-	denotes	;
T19622	12688-12691	RB	denotes	not
T19623	12692-12700	VB	denotes	depicted
T19624	12700-12701	-RRB-	denotes	)
T19625	12701-12702	-LRB-	denotes	.
T19626	12703-12704	NN	denotes	(
T19627	12704-12705	-RRB-	denotes	C
T19628	12705-12713	NN	denotes	) Granzy
T19629	12713-12714	NN	denotes	m
T19630	12714-12717	CC	denotes	e B
T19631	12718-12723	NN	denotes	and I
T19632	12723-12733	NN	denotes	FN-γ expre
T19633	12733-12735	IN	denotes	ss
T19634	12735-12743	NN	denotes	ion by T
T19635	12743-12746	CC	denotes	bx2
T19636	12746-12754	NN	denotes	1+/+ and
T19637	12755-12756	NN	denotes	T
T19638	12756-12761	NN	denotes	bx21−
T19639	12761-12769	VB	denotes	/− T cel
T19640	12769-12771	IN	denotes	ls
T19641	12772-12784	VB	denotes	analyzed in
T19642	12784-12789	NN	denotes	resti
T19643	12789-12793	WDT	denotes	mula
T19644	12793-12796	VB	denotes	ted
T19645	12797-12801	VB	denotes	cell
T19646	12801-12809	VB	denotes	s that h
T19647	12809-12812	IN	denotes	ad
T19648	12812-12813	CD	denotes	b
T19649	12813-12815	NN	denotes	ee
T19650	12815-12816	-LRB-	denotes	n
T19651	12817-12818	NN	denotes	c
T19652	12818-12819	-RRB-	denotes	u
T19653	12819-12824	NN	denotes	lture
T19654	12824-12834	NN	denotes	d for 5 d.
T19655	12835-12837	IN	denotes	(D
T19656	12837-12842	NN	denotes	) IFN
T19657	12842-12852	VB	denotes	-γ product
T19658	12852-12856	IN	denotes	ion
T19659	12856-12863	NN	denotes	by cell
T19660	12863-12865	CC	denotes	s
T19661	12865-12872	NN	denotes	transdu
T19662	12872-12873	-LRB-	denotes	c
T19663	12873-12876	NN	denotes	ed
T19664	12876-12877	-RRB-	denotes	w
T19665	12877-12889	NN	denotes	ith Eo-VP16
T19666	12889-12890	-LRB-	denotes	o
T19667	12890-12892	NN	denotes	r
T19668	12892-12893	-RRB-	denotes	c
T19669	12893-12901	VB	denotes	ontrol (
T19670	12901-12903	IN	denotes	GF
T19671	12903-12906	NN	denotes	P)
T19672	12906-12907	CD	denotes	r
T19673	12907-12912	IN	denotes	etrov
T19674	12912-12913	CD	denotes	i
T19675	12913-12914	NN	denotes	r
T19676	12914-12916	IN	denotes	us
T19677	12916-12929	NN	denotes	es (RV) measu
T19678	12929-12933	IN	denotes	red
T19679	12933-12936	NN	denotes	on
T19680	12936-12939	CC	denotes	day
T19681	12940-12949	NN	denotes	4 after 6
T19682	12993-13000	NN	denotes	Numbers
T19683	13001-13005	VB	denotes	show
T19684	13006-13009	DT	denotes	the
T19685	13010-13020	NN	denotes	percentage
T19686	13021-13023	IN	denotes	of
T19687	13024-13028	JJ	denotes	GFP+
T19688	13029-13035	JJ	denotes	IFN-γ+
T19689	13036-13041	NN	denotes	cells
T19690	13043-13050	NN	denotes	Results
T19691	13051-13054	VB	denotes	are
T19692	13055-13069	JJ	denotes	representative
T19693	13070-13072	IN	denotes	of
T19694	13073-13078	CD	denotes	three
T19695	13079-13080	-LRB-	denotes	(
T19696	13080-13081	NN	denotes	A
T19697	13082-13085	CC	denotes	and
T19698	13086-13087	NN	denotes	C
T19699	13087-13088	-RRB-	denotes	)
T19700	13089-13091	CC	denotes	or
T19701	13092-13095	CD	denotes	two
T19702	13096-13097	-LRB-	denotes	(
T19703	13097-13098	NN	denotes	B
T19704	13099-13102	CC	denotes	and
T19705	13103-13104	NN	denotes	D
T19706	13104-13105	-RRB-	denotes	)
T19707	13106-13117	JJ	denotes	independent
T19708	13118-13129	NN	denotes	experiments
T6689	13130-13132	TO	denotes	To
T6690	13133-13140	VB	denotes	examine
T6691	13141-13144	DT	denotes	the
T6692	13145-13149	NN	denotes	role
T6693	13150-13152	IN	denotes	of
T6694	13153-13158	NN	denotes	Eomes
T6695	13158-13159	-COMMA-	denotes	,
T6696	13160-13162	PRP	denotes	we
T6697	13163-13173	VB	denotes	transduced
T6698	13174-13179	JJ	denotes	naive
T6699	13180-13184	JJ	denotes	CD8+
T6700	13185-13186	NN	denotes	T
T6701	13187-13192	NN	denotes	cells
T6702	13193-13197	IN	denotes	from
T6703	13198-13200	NN	denotes	WT
T6704	13201-13204	CC	denotes	and
T6705	13205-13213	NN	denotes	Tbx21−/−
T6706	13214-13218	NN	denotes	mice
T6707	13219-13223	IN	denotes	with
T6708	13224-13236	NN	denotes	retroviruses
T6709	13237-13247	VB	denotes	containing
T6710	13248-13256	JJ	denotes	internal
T6711	13257-13265	NN	denotes	ribosome
T6712	13266-13271	NN	denotes	entry
T6713	13272-13276	NN	denotes	site
T6714	13277-13278	-LRB-	denotes	(
T6715	13278-13282	NN	denotes	IRES
T6716	13282-13283	-RRB-	denotes	)
T6717	13283-13287	NN	denotes	–GFP
T6718	13288-13292	WDT	denotes	that
T6719	13293-13297	VB	denotes	were
T6720	13298-13304	CC	denotes	either
T6721	13305-13310	JJ	denotes	empty
T6722	13311-13313	CC	denotes	or
T6723	13314-13321	VB	denotes	encoded
T6724	13322-13323	DT	denotes	a
T6725	13324-13332	RB	denotes	strongly
T6726	13333-13348	VB	denotes	transactivating
T6727	13349-13356	NN	denotes	version
T6728	13357-13359	IN	denotes	of
T6729	13360-13365	NN	denotes	Eomes
T6730	13366-13367	-LRB-	denotes	(
T6731	13367-13374	NN	denotes	Eo-VP16
T6732	13374-13375	-RRB-	denotes	)
T6733	13376-13377	-LRB-	denotes	(
T6734	13377-13378	CD	denotes	8
T6735	13378-13379	-RRB-	denotes	)
T6736	13379-13380	-COMMA-	denotes	,
T6737	13381-13384	CC	denotes	and
T6738	13385-13393	VB	denotes	expanded
T6739	13394-13398	PRP	denotes	them
T6740	13399-13402	IN	denotes	for
T6741	13403-13404	CD	denotes	6
T6742	13405-13406	NN	denotes	d
T6743	13407-13412	IN	denotes	under
T6744	13413-13416	PRP-DOLLAR-	denotes	our
T6745	13417-13424	NN	denotes	culture
T6746	13425-13435	NN	denotes	conditions
T6747	13437-13444	NN	denotes	Eo-VP16
T6748	13444-13445	-COMMA-	denotes	,
T6749	13446-13449	CC	denotes	but
T6750	13450-13453	RB	denotes	not
T6751	13454-13457	DT	denotes	the
T6752	13458-13463	JJ	denotes	empty
T6753	13464-13467	NN	denotes	GFP
T6754	13468-13478	NN	denotes	retrovirus
T6755	13478-13479	-COMMA-	denotes	,
T6756	13480-13489	VB	denotes	increased
T6757	13490-13498	NN	denotes	perforin
T6758	13499-13509	NN	denotes	expression
T6759	13510-13512	IN	denotes	in
T6760	13513-13517	CC	denotes	both
T6761	13518-13520	NN	denotes	WT
T6762	13521-13524	CC	denotes	and
T6763	13525-13540	JJ	denotes	T-bet–deficient
T6764	13541-13545	JJ	denotes	CD8+
T6765	13546-13547	NN	denotes	T
T6766	13548-13553	NN	denotes	cells
T6767	13554-13555	-LRB-	denotes	(
T6768	13555-13559	NN	denotes	Fig.
T6769	13560-13561	CD	denotes	2
T6770	13562-13563	NN	denotes	B
T6771	13563-13564	-COMMA-	denotes	,
T6772	13565-13570	NN	denotes	lanes
T6773	13571-13572	CD	denotes	2
T6774	13572-13573	-COMMA-	denotes	,
T6775	13574-13575	CD	denotes	3
T6776	13575-13576	-COMMA-	denotes	,
T6777	13577-13578	CD	denotes	5
T6778	13578-13579	-COMMA-	denotes	,
T6779	13580-13583	CC	denotes	and
T6780	13584-13585	CD	denotes	6
T6781	13585-13586	-RRB-	denotes	)
T6782	13588-13590	IN	denotes	As
T6783	13591-13599	VB	denotes	expected
T6784	13599-13600	-COMMA-	denotes	,
T6785	13601-13608	NN	denotes	Eo-VP16
T6786	13609-13613	RB	denotes	also
T6787	13614-13621	VB	denotes	rescued
T6788	13622-13625	DT	denotes	the
T6789	13626-13631	JJ	denotes	early
T6790	13632-13638	NN	denotes	defect
T6791	13639-13641	IN	denotes	in
T6792	13642-13647	NN	denotes	IFN-γ
T6793	13648-13658	NN	denotes	production
T6794	13659-13667	VB	denotes	observed
T6795	13668-13670	IN	denotes	in
T6796	13671-13686	JJ	denotes	T-bet–deficient
T6797	13687-13691	JJ	denotes	CD8+
T6798	13692-13693	NN	denotes	T
T6799	13694-13699	NN	denotes	cells
T6800	13700-13701	-LRB-	denotes	(
T6801	13701-13705	NN	denotes	Fig.
T6802	13706-13707	CD	denotes	2
T6803	13708-13709	NN	denotes	D
T6804	13709-13710	-RRB-	denotes	)
T6805	13712-13719	RB	denotes	However
T6806	13719-13720	-COMMA-	denotes	,
T6807	13721-13728	NN	denotes	Eo-VP16
T6808	13729-13732	VB	denotes	did
T6809	13733-13736	RB	denotes	not
T6810	13737-13743	VB	denotes	induce
T6811	13744-13748	NN	denotes	GzmB
T6812	13749-13753	NN	denotes	mRNA
T6813	13754-13764	NN	denotes	expression
T6814	13765-13767	IN	denotes	in
T6815	13768-13774	CC	denotes	either
T6816	13775-13777	NN	denotes	WT
T6817	13778-13780	CC	denotes	or
T6818	13781-13796	JJ	denotes	T-bet–deficient
T6819	13797-13802	NN	denotes	cells
T6820	13802-13803	-COLON-	denotes	;
T6821	13804-13808	RB	denotes	thus
T6822	13808-13809	-COMMA-	denotes	,
T6823	13810-13813	DT	denotes	the
T6824	13814-13821	JJ	denotes	partial
T6825	13822-13827	NN	denotes	T-bet
T6826	13828-13838	NN	denotes	dependence
T6827	13839-13841	IN	denotes	of
T6828	13842-13846	NN	denotes	GzmB
T6829	13847-13851	NN	denotes	mRNA
T6830	13852-13862	NN	denotes	expression
T6831	13863-13866	MD	denotes	can
T6832	13866-13869	RB	denotes	not
T6833	13870-13872	VB	denotes	be
T6834	13873-13884	VB	denotes	compensated
T6835	13885-13888	IN	denotes	for
T6836	13889-13891	IN	denotes	by
T6837	13892-13899	NN	denotes	Eo-VP16
T8977	13902-13907	NN	denotes	Runx3
T8978	13908-13916	VB	denotes	controls
T8979	13917-13925	JJ	denotes	multiple
T8980	13926-13933	NN	denotes	aspects
T8981	13934-13936	IN	denotes	of
T8982	13937-13940	DT	denotes	the
T8983	13941-13944	NN	denotes	CTL
T8984	13945-13960	NN	denotes	differentiation
T8985	13961-13968	NN	denotes	program
T8986	13968-13969	-COMMA-	denotes	,
T8987	13970-13972	IN	denotes	in
T8988	13973-13977	NN	denotes	part
T8989	13978-13985	IN	denotes	through
T8990	13986-13995	NN	denotes	induction
T8991	13996-13998	IN	denotes	of
T8992	13999-14004	NN	denotes	Eomes
T8993	14005-14012	IN	denotes	Because
T8994	14013-14018	NN	denotes	Runx3
T8995	14019-14021	VB	denotes	is
T8996	14022-14028	RB	denotes	highly
T8997	14029-14038	VB	denotes	expressed
T8998	14039-14041	IN	denotes	in
T8999	14042-14052	JJ	denotes	peripheral
T9000	14053-14057	JJ	denotes	CD8+
T9001	14058-14059	NN	denotes	T
T9002	14060-14065	NN	denotes	cells
T9003	14065-14066	-COMMA-	denotes	,
T9004	14067-14070	CC	denotes	and
T9005	14071-14078	IN	denotes	because
T9006	14079-14081	IN	denotes	of
T9007	14082-14085	DT	denotes	the
T9008	14086-14097	NN	denotes	T-bet-Runx3
T9009	14098-14109	NN	denotes	cooperation
T9010	14110-14112	PRP	denotes	we
T9011	14113-14121	VB	denotes	observed
T9012	14122-14129	RB	denotes	earlier
T9013	14130-14132	IN	denotes	in
T9014	14133-14137	JJ	denotes	CD4+
T9015	14138-14139	NN	denotes	T
T9016	14140-14145	NN	denotes	cells
T9017	14146-14147	-LRB-	denotes	(
T9018	14147-14149	CD	denotes	15
T9019	14149-14150	-RRB-	denotes	)
T9020	14150-14151	-COMMA-	denotes	,
T9021	14152-14154	PRP	denotes	we
T9022	14155-14163	VB	denotes	examined
T9023	14164-14167	DT	denotes	the
T9024	14168-14172	NN	denotes	role
T9025	14173-14175	IN	denotes	of
T9026	14176-14181	NN	denotes	Runx3
T9027	14182-14184	IN	denotes	in
T9028	14185-14193	NN	denotes	effector
T9029	14194-14197	NN	denotes	CTL
T9030	14198-14213	NN	denotes	differentiation
T9031	14215-14217	PRP	denotes	We
T9032	14218-14226	VB	denotes	isolated
T9033	14227-14231	JJ	denotes	CD8+
T9034	14232-14233	NN	denotes	T
T9035	14234-14239	NN	denotes	cells
T9036	14240-14244	IN	denotes	from
T9037	14245-14253	NN	denotes	Runx3−/−
T9038	14254-14255	-LRB-	denotes	(
T9039	14255-14257	NN	denotes	KO
T9040	14257-14258	-RRB-	denotes	)
T9041	14259-14263	NN	denotes	mice
T9042	14264-14266	IN	denotes	of
T9043	14267-14270	DT	denotes	the
T9044	14271-14278	JJ	denotes	outbred
T9045	14279-14282	NN	denotes	ICR
T9046	14283-14293	NN	denotes	background
T9047	14294-14297	CC	denotes	and
T9048	14298-14303	PRP-DOLLAR-	denotes	their
T9049	14304-14306	JJ	denotes	WT
T9050	14307-14315	NN	denotes	Runx3+/+
T9051	14316-14327	NN	denotes	littermates
T9052	14328-14330	IN	denotes	by
T9053	14331-14339	JJ	denotes	positive
T9054	14340-14349	NN	denotes	selection
T9055	14350-14354	IN	denotes	with
T9056	14355-14363	JJ	denotes	anti-CD8
T9057	14364-14372	JJ	denotes	magnetic
T9058	14373-14378	NN	denotes	beads
T9059	14379-14380	-LRB-	denotes	(
T9060	14380-14385	NN	denotes	Figs.
T9061	14386-14388	NN	denotes	S1
T9062	14389-14392	CC	denotes	and
T9063	14393-14395	NN	denotes	S2
T9064	14395-14396	-COMMA-	denotes	,
T9065	14397-14406	JJ	denotes	available
T9066	14407-14409	IN	denotes	at
T9067	14410-14414	NN	denotes	http
T9068	14414-14415	-COLON-	denotes	:
T9069	14415-14462	NN	denotes	//www.jem.org/cgi/content/full/jem.20081242/DC1
T9070	14462-14463	-RRB-	denotes	)
T9071	14465-14475	RB	denotes	Strikingly
T9072	14475-14476	-COMMA-	denotes	,
T9073	14477-14485	NN	denotes	Runx3−/−
T9074	14486-14490	JJ	denotes	CD8+
T9075	14491-14492	NN	denotes	T
T9076	14493-14498	NN	denotes	cells
T9077	14499-14503	VB	denotes	were
T9078	14504-14512	RB	denotes	strongly
T9079	14513-14521	JJ	denotes	impaired
T9080	14522-14524	IN	denotes	in
T9081	14525-14530	PRP-DOLLAR-	denotes	their
T9082	14531-14538	NN	denotes	ability
T9083	14539-14541	TO	denotes	to
T9084	14542-14555	VB	denotes	differentiate
T9085	14556-14560	IN	denotes	into
T9086	14561-14569	NN	denotes	effector
T9087	14570-14574	NN	denotes	CTLs
T9088	14574-14575	-COMMA-	denotes	,
T9089	14576-14578	IN	denotes	as
T9090	14579-14585	VB	denotes	judged
T9091	14586-14588	IN	denotes	by
T9092	14589-14599	NN	denotes	expression
T9093	14600-14602	IN	denotes	of
T9094	14603-14611	NN	denotes	perforin
T9095	14611-14612	-COMMA-	denotes	,
T9096	14613-14621	NN	denotes	granzyme
T9097	14622-14623	NN	denotes	B
T9098	14623-14624	-COMMA-	denotes	,
T9099	14625-14628	CC	denotes	and
T9100	14629-14634	NN	denotes	IFN-γ
T9101	14635-14636	-LRB-	denotes	(
T9102	14636-14640	NN	denotes	Fig.
T9103	14641-14642	CD	denotes	3
T9104	14642-14643	-RRB-	denotes	)
T9105	14645-14653	VB	denotes	Compared
T9106	14654-14658	IN	denotes	with
T9107	14659-14661	JJ	denotes	WT
T9108	14662-14663	NN	denotes	T
T9109	14664-14669	NN	denotes	cells
T9110	14669-14670	-COMMA-	denotes	,
T9111	14671-14679	NN	denotes	perforin
T9112	14680-14684	NN	denotes	mRNA
T9113	14685-14688	CC	denotes	and
T9114	14689-14696	NN	denotes	protein
T9115	14697-14707	NN	denotes	expression
T9116	14708-14712	VB	denotes	were
T9117	14713-14724	RB	denotes	essentially
T9118	14725-14737	JJ	denotes	undetectable
T9119	14738-14740	IN	denotes	in
T9120	14741-14749	NN	denotes	Runx3−/−
T9121	14750-14751	NN	denotes	T
T9122	14752-14757	NN	denotes	cells
T9123	14758-14760	IN	denotes	at
T9124	14761-14764	NN	denotes	day
T9125	14765-14766	CD	denotes	6
T9126	14767-14769	IN	denotes	of
T9127	14770-14777	NN	denotes	culture
T9128	14778-14779	-LRB-	denotes	(
T9129	14779-14783	NNP	denotes	Fig.
T9130	14784-14785	CD	denotes	3
T9131	14785-14786	-COMMA-	denotes	,
T9132	14787-14788	NN	denotes	A
T9133	14789-14792	CC	denotes	and
T9134	14793-14794	NN	denotes	B
T9135	14794-14795	-RRB-	denotes	)
T9136	14797-14805	NN	denotes	Runx3−/−
T9137	14806-14807	NN	denotes	T
T9138	14808-14813	NN	denotes	cells
T9139	14814-14818	RB	denotes	also
T9140	14819-14822	VB	denotes	had
T9141	14823-14825	DT	denotes	no
T9142	14826-14836	JJ	denotes	detectable
T9143	14837-14842	NN	denotes	Eomes
T9144	14843-14853	NN	denotes	expression
T9145	14853-14854	-COLON-	denotes	;
T9146	14855-14857	IN	denotes	in
T9147	14858-14866	NN	denotes	contrast
T9148	14866-14867	-COMMA-	denotes	,
T9149	14868-14873	NN	denotes	T-bet
T9150	14874-14884	NN	denotes	expression
T9151	14885-14888	VB	denotes	was
T9152	14889-14899	JJ	denotes	unimpaired
T9153	14900-14901	-LRB-	denotes	(
T9154	14901-14905	NN	denotes	Fig.
T9155	14906-14907	CD	denotes	3
T9156	14908-14909	NN	denotes	A
T9157	14909-14910	-RRB-	denotes	)
T9158	14912-14923	RB	denotes	Furthermore
T9159	14923-14924	-COMMA-	denotes	,
T9160	14925-14930	NN	denotes	Runx3
T9161	14931-14934	VB	denotes	was
T9162	14935-14943	VB	denotes	required
T9163	14944-14947	IN	denotes	for
T9164	14948-14955	JJ	denotes	maximal
T9165	14956-14966	NN	denotes	production
T9166	14967-14969	IN	denotes	of
T9167	14970-14975	NN	denotes	IFN-γ
T9168	14975-14976	-COMMA-	denotes	,
T9169	14977-14980	CC	denotes	but
T9170	14981-14984	RB	denotes	not
T9171	14985-14988	NN	denotes	TNF
T9172	14989-14991	CC	denotes	or
T9173	14992-14996	NN	denotes	IL-2
T9174	14996-14997	-COMMA-	denotes	,
T9175	14998-15000	IN	denotes	by
T9176	15001-15005	JJ	denotes	CD8+
T9177	15006-15007	NN	denotes	T
T9178	15008-15013	NN	denotes	cells
T9179	15014-15026	VB	denotes	restimulated
T9180	15027-15029	IN	denotes	at
T9181	15030-15033	NN	denotes	day
T9182	15034-15035	CD	denotes	6
T9183	15036-15037	-LRB-	denotes	(
T9184	15037-15041	NN	denotes	Fig.
T9185	15042-15043	CD	denotes	3
T9186	15044-15045	NN	denotes	C
T9187	15045-15046	-RRB-	denotes	)
T9188	16186-16188	PRP	denotes	We
T9189	16189-16199	RB	denotes	previously
T9190	16200-16208	VB	denotes	reported
T9191	16209-16213	IN	denotes	that
T9192	16214-16217	NN	denotes	Th1
T9193	16218-16222	NN	denotes	cell
T9194	16223-16238	NN	denotes	differentiation
T9195	16239-16242	VB	denotes	was
T9196	16243-16252	VB	denotes	regulated
T9197	16253-16260	IN	denotes	through
T9198	16261-16262	DT	denotes	a
T9199	16263-16275	JJ	denotes	feed-forward
T9200	16276-16280	NN	denotes	loop
T9201	16281-16283	IN	denotes	in
T9202	16284-16289	WDT	denotes	which
T9203	16290-16295	NN	denotes	T-bet
T9204	16296-16298	VB	denotes	is
T9205	16299-16311	VB	denotes	up-regulated
T9206	16312-16317	RB	denotes	early
T9207	16318-16321	CC	denotes	and
T9208	16322-16329	VB	denotes	induces
T9209	16330-16335	NN	denotes	Runx3
T9210	16335-16336	-COMMA-	denotes	,
T9211	16337-16342	IN	denotes	after
T9212	16343-16348	WDT	denotes	which
T9213	16349-16354	NN	denotes	T-bet
T9214	16355-16358	CC	denotes	and
T9215	16359-16364	NN	denotes	Runx3
T9216	16365-16374	VB	denotes	cooperate
T9217	16375-16377	TO	denotes	to
T9218	16378-16384	VB	denotes	induce
T9219	16385-16390	NN	denotes	IFN-γ
T9220	16391-16394	CC	denotes	and
T9221	16395-16402	NN	denotes	silence
T9222	16403-16407	NN	denotes	IL-4
T9223	16407-16408	-COMMA-	denotes	,
T9224	16409-16413	RB	denotes	thus
T9225	16414-16423	VB	denotes	promoting
T9226	16424-16430	JJ	denotes	stable
T9227	16431-16446	NN	denotes	differentiation
T9228	16447-16453	IN	denotes	toward
T9229	16454-16457	DT	denotes	the
T9230	16458-16461	NN	denotes	Th1
T9231	16462-16469	NN	denotes	lineage
T9232	16470-16471	-LRB-	denotes	(
T9233	16471-16473	CD	denotes	15
T9234	16473-16474	-COMMA-	denotes	,
T9235	16475-16477	CD	denotes	22
T9236	16477-16478	-RRB-	denotes	)
T9237	16480-16487	IN	denotes	Because
T9238	16488-16489	-LRB-	denotes	(
T9239	16489-16490	DT	denotes	a
T9240	16490-16491	-RRB-	denotes	)
T9241	16492-16497	NN	denotes	Runx3
T9242	16498-16506	VB	denotes	appeared
T9243	16507-16516	JJ	denotes	necessary
T9244	16517-16520	IN	denotes	for
T9245	16521-16526	NN	denotes	Eomes
T9246	16527-16536	NN	denotes	induction
T9247	16537-16538	-LRB-	denotes	(
T9248	16538-16542	NN	denotes	Fig.
T9249	16543-16544	CD	denotes	3
T9250	16545-16546	NN	denotes	A
T9251	16546-16547	-RRB-	denotes	)
T9252	16547-16548	-COMMA-	denotes	,
T9253	16549-16550	-LRB-	denotes	(
T9254	16550-16551	NN	denotes	b
T9255	16551-16552	-RRB-	denotes	)
T9256	16553-16556	DT	denotes	the
T9257	16557-16565	NN	denotes	kinetics
T9258	16566-16568	IN	denotes	of
T9259	16569-16574	NN	denotes	Eomes
T9260	16575-16585	NN	denotes	expression
T9261	16586-16596	VB	denotes	paralleled
T9262	16597-16602	DT	denotes	those
T9263	16603-16605	IN	denotes	of
T9264	16606-16614	NN	denotes	perforin
T9265	16615-16625	NN	denotes	expression
T9266	16626-16627	-LRB-	denotes	(
T9267	16627-16631	NN	denotes	Fig.
T9268	16632-16633	CD	denotes	2
T9269	16633-16634	-RRB-	denotes	)
T9270	16634-16635	-COMMA-	denotes	,
T9271	16636-16639	CC	denotes	and
T9272	16640-16641	-LRB-	denotes	(
T9273	16641-16642	NN	denotes	c
T9274	16642-16643	-RRB-	denotes	)
T9275	16644-16658	NN	denotes	overexpression
T9276	16659-16661	IN	denotes	of
T9277	16662-16669	NN	denotes	Eo-VP16
T9278	16670-16672	IN	denotes	in
T9279	16673-16679	CC	denotes	either
T9280	16680-16682	NN	denotes	WT
T9281	16683-16685	CC	denotes	or
T9282	16686-16701	JJ	denotes	T-bet–deficient
T9283	16702-16703	NN	denotes	T
T9284	16704-16709	NN	denotes	cells
T9285	16710-16713	VB	denotes	led
T9286	16714-16716	TO	denotes	to
T9287	16717-16719	DT	denotes	an
T9288	16720-16728	NN	denotes	increase
T9289	16729-16731	IN	denotes	in
T9290	16732-16736	CC	denotes	both
T9291	16737-16745	NN	denotes	perforin
T9292	16746-16749	CC	denotes	and
T9293	16750-16755	NN	denotes	IFN-γ
T9294	16756-16766	NN	denotes	expression
T9295	16767-16768	-LRB-	denotes	(
T9296	16768-16772	NN	denotes	Fig.
T9297	16773-16774	CD	denotes	2
T9298	16774-16775	-COMMA-	denotes	,
T9299	16776-16777	NN	denotes	B
T9300	16778-16781	CC	denotes	and
T9301	16782-16783	NN	denotes	D
T9302	16783-16784	-RRB-	denotes	)
T9303	16784-16785	-COMMA-	denotes	,
T9304	16786-16788	PRP	denotes	we
T9305	16789-16794	VB	denotes	asked
T9306	16795-16802	IN	denotes	whether
T9307	16803-16806	NN	denotes	CTL
T9308	16807-16822	NN	denotes	differentiation
T9309	16823-16826	VB	denotes	was
T9310	16827-16831	RB	denotes	also
T9311	16832-16843	RB	denotes	potentially
T9312	16844-16853	VB	denotes	regulated
T9313	16854-16856	IN	denotes	by
T9314	16857-16858	DT	denotes	a
T9315	16859-16871	JJ	denotes	feed-forward
T9316	16872-16876	NN	denotes	loop
T9317	16877-16886	VB	denotes	involving
T9318	16887-16892	DT	denotes	these
T9319	16893-16897	JJ	denotes	same
T9320	16898-16901	CD	denotes	two
T9321	16902-16909	NN	denotes	classes
T9322	16910-16912	IN	denotes	of
T9323	16913-16917	NN	denotes	Runx
T9324	16918-16921	CC	denotes	and
T9325	16922-16927	NN	denotes	T-box
T9326	16928-16941	NN	denotes	transcription
T9327	16942-16949	NN	denotes	factors
T9328	16951-16963	RB	denotes	Specifically
T9329	16963-16964	-COMMA-	denotes	,
T9330	16965-16967	PRP	denotes	we
T9331	16968-16973	VB	denotes	asked
T9332	16974-16981	IN	denotes	whether
T9333	16982-16987	NN	denotes	Runx3
T9334	16987-16988	-COMMA-	denotes	,
T9335	16989-16994	WDT	denotes	which
T9336	16995-16998	VB	denotes	was
T9337	16999-17008	JJ	denotes	necessary
T9338	17009-17012	IN	denotes	for
T9339	17013-17018	NN	denotes	Eomes
T9340	17019-17028	NN	denotes	induction
T9341	17028-17029	-COMMA-	denotes	,
T9342	17030-17034	RB	denotes	then
T9343	17035-17045	VB	denotes	cooperated
T9344	17046-17050	IN	denotes	with
T9345	17051-17056	NN	denotes	Eomes
T9346	17057-17059	TO	denotes	to
T9347	17060-17068	VB	denotes	regulate
T9348	17069-17082	NN	denotes	transcription
T9349	17083-17085	IN	denotes	of
T9350	17086-17089	DT	denotes	the
T9351	17090-17098	NN	denotes	effector
T9352	17099-17102	NN	denotes	CTL
T9353	17103-17110	NN	denotes	markers
T9354	17111-17119	NN	denotes	perforin
T9355	17119-17120	-COMMA-	denotes	,
T9356	17121-17126	NN	denotes	IFN-γ
T9357	17126-17127	-COMMA-	denotes	,
T9358	17128-17131	CC	denotes	and
T9359	17132-17140	NN	denotes	granzyme
T9360	17141-17142	NN	denotes	B
T9361	17144-17146	TO	denotes	To
T9362	17147-17151	VB	denotes	test
T9363	17152-17156	DT	denotes	this
T9364	17157-17167	NN	denotes	hypothesis
T9365	17167-17168	-COMMA-	denotes	,
T9366	17169-17171	PRP	denotes	we
T9367	17172-17176	VB	denotes	used
T9368	17177-17186	NN	denotes	chromatin
T9369	17187-17206	NN	denotes	immunoprecipitation
T9370	17207-17208	-LRB-	denotes	(
T9371	17208-17212	NN	denotes	ChIP
T9372	17212-17213	-RRB-	denotes	)
T9373	17214-17220	NN	denotes	assays
T9374	17221-17223	TO	denotes	to
T9375	17224-17227	VB	denotes	ask
T9376	17228-17235	IN	denotes	whether
T9377	17236-17241	NN	denotes	Eomes
T9378	17242-17245	CC	denotes	and
T9379	17246-17251	NN	denotes	Runx3
T9380	17252-17257	VB	denotes	bound
T9381	17258-17268	JJ	denotes	regulatory
T9382	17269-17276	NN	denotes	regions
T9383	17277-17279	IN	denotes	of
T9384	17280-17283	DT	denotes	the
T9385	17284-17288	NN	denotes	Prf1
T9386	17288-17289	-COMMA-	denotes	,
T9387	17290-17294	NN	denotes	Ifng
T9388	17294-17295	-COMMA-	denotes	,
T9389	17296-17299	CC	denotes	and
T9390	17300-17304	NN	denotes	Gzmb
T9391	17305-17310	NN	denotes	genes
T9392	17311-17312	-LRB-	denotes	(
T9393	17312-17316	NN	denotes	Fig.
T9394	17317-17318	CD	denotes	3
T9395	17319-17320	NN	denotes	D
T9396	17320-17321	-RRB-	denotes	)
T9397	17323-17327	DT	denotes	Both
T9398	17328-17336	NN	denotes	proteins
T9399	17337-17347	VB	denotes	associated
T9400	17348-17352	IN	denotes	with
T9401	17353-17357	NN	denotes	gene
T9402	17358-17368	JJ	denotes	regulatory
T9403	17369-17376	NN	denotes	regions
T9404	17377-17379	IN	denotes	in
T9405	17380-17394	VB	denotes	differentiated
T9406	17395-17399	NN	denotes	CTLs
T9407	17401-17406	NN	denotes	Runx3
T9408	17407-17412	VB	denotes	bound
T9409	17413-17415	TO	denotes	to
T9410	17416-17419	DT	denotes	the
T9411	17420-17424	NN	denotes	Prf1
T9412	17425-17428	CC	denotes	and
T9413	17429-17433	NN	denotes	Gzmb
T9414	17434-17447	NN	denotes	transcription
T9415	17448-17453	NN	denotes	start
T9416	17454-17459	NN	denotes	sites
T9417	17460-17461	-LRB-	denotes	(
T9418	17461-17464	NN	denotes	TSS
T9419	17464-17465	-RRB-	denotes	)
T9420	17465-17466	-COLON-	denotes	;
T9421	17467-17469	TO	denotes	to
T9422	17470-17471	DT	denotes	a
T9423	17472-17477	VB	denotes	known
T9424	17478-17482	NN	denotes	IL-2
T9425	17483-17493	JJ	denotes	responsive
T9426	17494-17502	NN	denotes	enhancer
T9427	17503-17510	JJ	denotes	located
T9428	17511-17515	IN	denotes	near
T9429	17516-17518	NN	denotes	−1
T9430	17519-17521	NN	denotes	kb
T9431	17522-17524	IN	denotes	of
T9432	17525-17528	DT	denotes	the
T9433	17529-17533	NN	denotes	Prf1
T9434	17534-17538	NN	denotes	gene
T9435	17539-17540	-LRB-	denotes	(
T9436	17540-17542	CD	denotes	23
T9437	17542-17543	-RRB-	denotes	)
T9438	17543-17544	-COLON-	denotes	;
T9439	17545-17547	TO	denotes	to
T9440	17548-17551	DT	denotes	the
T9441	17552-17558	JJ	denotes	distal
T9442	17559-17571	JJ	denotes	CTL-specific
T9443	17572-17577	NN	denotes	DNase
T9444	17578-17579	CD	denotes	I
T9445	17580-17594	JJ	denotes	hypersensitive
T9446	17595-17599	NN	denotes	site
T9447	17600-17601	CD	denotes	9
T9448	17602-17604	IN	denotes	in
T9449	17605-17608	DT	denotes	the
T9450	17609-17613	NN	denotes	Prf1
T9451	17614-17619	NN	denotes	locus
T9452	17620-17621	-LRB-	denotes	(
T9453	17621-17623	CD	denotes	24
T9454	17623-17624	-RRB-	denotes	)
T9455	17624-17625	-COLON-	denotes	;
T9456	17626-17628	TO	denotes	to
T9457	17629-17632	DT	denotes	the
T9458	17633-17637	NN	denotes	Ifng
T9459	17638-17646	NN	denotes	promoter
T9460	17647-17651	IN	denotes	near
T9461	17652-17655	DT	denotes	the
T9462	17656-17659	NN	denotes	TSS
T9463	17659-17660	-COMMA-	denotes	,
T9464	17661-17663	IN	denotes	as
T9465	17664-17674	RB	denotes	previously
T9466	17675-17683	VB	denotes	reported
T9467	17684-17687	IN	denotes	for
T9468	17688-17691	NN	denotes	Th1
T9469	17692-17697	NN	denotes	cells
T9470	17698-17699	-LRB-	denotes	(
T9471	17699-17701	CD	denotes	10
T9472	17701-17702	-RRB-	denotes	)
T9473	17702-17703	-COLON-	denotes	;
T9474	17704-17707	CC	denotes	and
T9475	17708-17710	TO	denotes	to
T9476	17711-17718	JJ	denotes	several
T9477	17719-17724	NN	denotes	DNase
T9478	17725-17726	CD	denotes	I
T9479	17727-17741	JJ	denotes	hypersensitive
T9480	17742-17747	NN	denotes	sites
T9481	17748-17750	IN	denotes	in
T9482	17751-17754	DT	denotes	the
T9483	17755-17759	NN	denotes	Ifng
T9484	17760-17765	NN	denotes	locus
T9485	17766-17767	-LRB-	denotes	(
T9486	17767-17771	NN	denotes	Fig.
T9487	17772-17773	CD	denotes	3
T9488	17774-17775	NN	denotes	D
T9489	17776-17779	CC	denotes	and
T9490	17780-17783	RB	denotes	not
T9491	17784-17792	VB	denotes	depicted
T9492	17792-17793	-RRB-	denotes	)
T9493	17794-17795	-LRB-	denotes	(
T9494	17795-17797	CD	denotes	25
T9495	17797-17798	-RRB-	denotes	)
T9496	17800-17805	NN	denotes	Eomes
T9497	17806-17811	VB	denotes	bound
T9498	17812-17821	RB	denotes	primarily
T9499	17822-17824	TO	denotes	to
T9500	17825-17828	DT	denotes	the
T9501	17829-17833	NN	denotes	Prf1
T9502	17834-17837	NN	denotes	TSS
T9503	17838-17841	CC	denotes	and
T9504	17842-17845	DT	denotes	the
T9505	17846-17848	NN	denotes	−1
T9506	17849-17851	NN	denotes	kb
T9507	17852-17860	NN	denotes	enhancer
T9508	17860-17861	-COLON-	denotes	;
T9509	17862-17866	DT	denotes	this
T9510	17867-17874	NN	denotes	binding
T9511	17875-17878	VB	denotes	was
T9512	17879-17892	RB	denotes	substantially
T9513	17893-17900	JJ	denotes	greater
T9514	17901-17905	IN	denotes	than
T9515	17906-17910	DT	denotes	that
T9516	17911-17919	VB	denotes	observed
T9517	17920-17922	IN	denotes	at
T9518	17923-17926	DT	denotes	the
T9519	17927-17935	NN	denotes	promoter
T9520	17936-17938	IN	denotes	of
T9521	17939-17942	DT	denotes	the
T9522	17943-17948	NN	denotes	Il2rb
T9523	17949-17953	NN	denotes	gene
T9524	17953-17954	-COMMA-	denotes	,
T9525	17955-17956	DT	denotes	a
T9526	17957-17962	JJ	denotes	known
T9527	17963-17969	JJ	denotes	direct
T9528	17970-17976	NN	denotes	target
T9529	17977-17979	IN	denotes	of
T9530	17980-17985	NN	denotes	Eomes
T9531	17986-17987	-LRB-	denotes	(
T9532	17987-17988	CD	denotes	8
T9533	17988-17989	-RRB-	denotes	)
T9534	17989-17990	-COMMA-	denotes	,
T9535	17991-17994	CC	denotes	and
T9536	17995-18005	JJ	denotes	comparable
T9537	18006-18008	TO	denotes	to
T9538	18009-18013	DT	denotes	that
T9539	18014-18022	VB	denotes	observed
T9540	18023-18025	IN	denotes	at
T9541	18026-18029	DT	denotes	the
T9542	18030-18034	NNP	denotes	Ifng
T9543	18035-18038	NN	denotes	TSS
T9544	18038-18039	-COMMA-	denotes	,
T9545	18040-18041	DT	denotes	a
T9546	18042-18047	VB	denotes	known
T9547	18048-18054	NN	denotes	target
T9548	18055-18057	IN	denotes	of
T9549	18058-18063	NN	denotes	T-box
T9550	18064-18072	NN	denotes	proteins
T9551	18073-18075	IN	denotes	in
T9552	18076-18080	CC	denotes	both
T9553	18081-18084	NN	denotes	Th1
T9554	18085-18088	CC	denotes	and
T9555	18089-18093	JJ	denotes	CD8+
T9556	18094-18095	NN	denotes	T
T9557	18096-18101	NN	denotes	cells
T9558	18102-18103	-LRB-	denotes	(
T9559	18103-18107	NN	denotes	Fig.
T9560	18108-18109	CD	denotes	3
T9561	18110-18111	NN	denotes	D
T9562	18111-18112	-RRB-	denotes	)
T9563	18113-18114	-LRB-	denotes	(
T9564	18114-18116	CD	denotes	17
T9565	18116-18117	-RRB-	denotes	)
T9566	18119-18121	TO	denotes	To
T9567	18122-18131	VB	denotes	determine
T9568	18132-18139	IN	denotes	whether
T9569	18140-18145	NN	denotes	Runx3
T9570	18146-18156	VB	denotes	controlled
T9571	18157-18160	DT	denotes	the
T9572	18161-18171	NN	denotes	expression
T9573	18172-18174	IN	denotes	of
T9574	18175-18178	NN	denotes	CTL
T9575	18179-18187	NN	denotes	effector
T9576	18188-18193	NN	denotes	genes
T9577	18194-18201	IN	denotes	through
T9578	18202-18205	PRP-DOLLAR-	denotes	its
T9579	18206-18215	NN	denotes	induction
T9580	18216-18218	IN	denotes	of
T9581	18219-18224	NN	denotes	Eomes
T9582	18224-18225	-COMMA-	denotes	,
T9583	18226-18228	PRP	denotes	we
T9584	18229-18241	RB	denotes	retrovirally
T9585	18242-18251	VB	denotes	expressed
T9586	18252-18257	NN	denotes	Runx3
T9587	18258-18261	CC	denotes	and
T9588	18262-18269	NN	denotes	Eo-VP16
T9589	18270-18272	IN	denotes	in
T9590	18273-18277	JJ	denotes	CD8+
T9591	18278-18279	NN	denotes	T
T9592	18280-18285	NN	denotes	cells
T9593	18286-18290	IN	denotes	from
T9594	18291-18299	NN	denotes	Runx3−/−
T9595	18300-18304	NN	denotes	mice
T9596	18306-18313	IN	denotes	Because
T9597	18314-18316	IN	denotes	of
T9598	18317-18320	DT	denotes	the
T9599	18321-18328	JJ	denotes	limited
T9600	18329-18335	NN	denotes	number
T9601	18336-18338	IN	denotes	of
T9602	18339-18343	JJ	denotes	CD8+
T9603	18344-18345	NN	denotes	T
T9604	18346-18351	NN	denotes	cells
T9605	18352-18354	IN	denotes	in
T9606	18355-18360	DT	denotes	these
T9607	18361-18365	NN	denotes	mice
T9608	18365-18366	-COMMA-	denotes	,
T9609	18367-18370	CC	denotes	and
T9610	18371-18378	IN	denotes	because
T9611	18379-18381	PRP	denotes	we
T9612	18382-18385	VB	denotes	saw
T9613	18386-18388	DT	denotes	no
T9614	18389-18399	NN	denotes	difference
T9615	18400-18407	IN	denotes	between
T9616	18408-18416	NN	denotes	Runx3−/−
T9617	18417-18425	NN	denotes	CD8+CD4−
T9618	18426-18428	NN	denotes	SP
T9619	18429-18432	CC	denotes	and
T9620	18433-18441	JJ	denotes	CD8+CD4+
T9621	18442-18444	NN	denotes	DP
T9622	18445-18450	NN	denotes	cells
T9623	18451-18453	IN	denotes	in
T9624	18454-18457	PRP-DOLLAR-	denotes	our
T9625	18458-18466	JJ	denotes	previous
T9626	18467-18478	NN	denotes	experiments
T9627	18478-18479	-COMMA-	denotes	,
T9628	18480-18482	PRP	denotes	we
T9629	18483-18487	VB	denotes	used
T9630	18488-18493	JJ	denotes	total
T9631	18494-18502	NN	denotes	Runx3−/−
T9632	18503-18507	JJ	denotes	CD8+
T9633	18508-18509	NN	denotes	T
T9634	18510-18515	NN	denotes	cells
T9635	18516-18523	IN	denotes	without
T9636	18524-18531	JJ	denotes	further
T9637	18532-18545	NN	denotes	fractionation
T9638	18546-18548	IN	denotes	as
T9639	18549-18559	NN	denotes	recipients
T9640	18560-18563	IN	denotes	for
T9641	18564-18574	JJ	denotes	retroviral
T9642	18575-18587	NN	denotes	transduction
T9643	18589-18603	NN	denotes	Reconstitution
T9644	18604-18606	IN	denotes	of
T9645	18607-18615	NN	denotes	Runx3−/−
T9646	18616-18620	JJ	denotes	CD8+
T9647	18621-18622	NN	denotes	T
T9648	18623-18628	NN	denotes	cells
T9649	18629-18633	IN	denotes	with
T9650	18634-18639	NN	denotes	Runx3
T9651	18640-18648	VB	denotes	restored
T9652	18649-18659	NN	denotes	expression
T9653	18660-18662	IN	denotes	of
T9654	18663-18668	NN	denotes	Eomes
T9655	18669-18671	RB	denotes	as
T9656	18672-18676	RB	denotes	well
T9657	18677-18679	IN	denotes	as
T9658	18680-18688	NN	denotes	perforin
T9659	18688-18689	-COMMA-	denotes	,
T9660	18690-18698	NN	denotes	granzyme
T9661	18699-18700	NN	denotes	B
T9662	18700-18701	-COMMA-	denotes	,
T9663	18702-18705	CC	denotes	and
T9664	18706-18711	NN	denotes	IFN-γ
T9665	18712-18713	-LRB-	denotes	(
T9666	18713-18717	NN	denotes	Fig.
T9667	18718-18719	CD	denotes	4
T9668	18719-18720	-COMMA-	denotes	,
T9669	18721-18722	NN	denotes	A
T9670	18723-18726	CC	denotes	and
T9671	18727-18728	NN	denotes	B
T9672	18728-18729	-RRB-	denotes	)
T9673	18731-18733	IN	denotes	In
T9674	18734-18742	NN	denotes	addition
T9675	18742-18743	-COMMA-	denotes	,
T9676	18744-18752	NN	denotes	Runx3−/−
T9677	18753-18754	NN	denotes	T
T9678	18755-18760	NN	denotes	cells
T9679	18761-18767	VB	denotes	showed
T9680	18768-18769	DT	denotes	a
T9681	18770-18782	JJ	denotes	compensatory
T9682	18783-18796	NN	denotes	up-regulation
T9683	18797-18799	IN	denotes	of
T9684	18800-18805	NN	denotes	Runx1
T9685	18805-18806	-COMMA-	denotes	,
T9686	18807-18812	WDT	denotes	which
T9687	18813-18816	VB	denotes	was
T9688	18817-18827	VB	denotes	suppressed
T9689	18828-18832	IN	denotes	upon
T9690	18833-18847	NN	denotes	reconstitution
T9691	18848-18852	IN	denotes	with
T9692	18853-18858	NN	denotes	Runx3
T9693	18858-18859	-COMMA-	denotes	,
T9694	18860-18870	VB	denotes	indicating
T9695	18871-18875	IN	denotes	that
T9696	18876-18881	NN	denotes	Runx1
T9697	18882-18884	VB	denotes	is
T9698	18885-18886	DT	denotes	a
T9699	18887-18893	NN	denotes	target
T9700	18894-18896	IN	denotes	of
T9701	18897-18907	NN	denotes	repression
T9702	18908-18910	IN	denotes	by
T9703	18911-18916	NN	denotes	Runx3
T9704	18918-18925	RB	denotes	Notably
T9705	18925-18926	-COMMA-	denotes	,
T9706	18927-18934	NN	denotes	Eo-VP16
T9707	18935-18938	VB	denotes	did
T9708	18939-18942	RB	denotes	not
T9709	18943-18954	VB	denotes	up-regulate
T9710	18955-18963	NN	denotes	perforin
T9711	18964-18974	NN	denotes	expression
T9712	18975-18979	WRB	denotes	when
T9713	18980-18989	VB	denotes	expressed
T9714	18990-18992	IN	denotes	in
T9715	18993-19001	NN	denotes	Runx3−/−
T9716	19002-19007	NN	denotes	cells
T9717	19007-19008	-COMMA-	denotes	,
T9718	19009-19013	RB	denotes	even
T9719	19014-19020	IN	denotes	though
T9720	19021-19023	PRP	denotes	it
T9721	19024-19032	VB	denotes	restored
T9722	19033-19036	DT	denotes	the
T9723	19037-19045	NN	denotes	capacity
T9724	19046-19048	TO	denotes	to
T9725	19049-19055	VB	denotes	induce
T9726	19056-19061	NN	denotes	IFN-γ
T9727	19062-19072	NN	denotes	expression
T9728	19073-19077	IN	denotes	upon
T9729	19078-19081	NN	denotes	TCR
T9730	19082-19095	NN	denotes	restimulation
T9731	19096-19097	-LRB-	denotes	(
T9732	19097-19101	NN	denotes	Fig.
T9733	19102-19103	CD	denotes	4
T9734	19103-19104	-COMMA-	denotes	,
T9735	19105-19106	NN	denotes	A
T9736	19107-19110	CC	denotes	and
T9737	19111-19112	NN	denotes	B
T9738	19112-19113	-RRB-	denotes	)
T9739	19115-19119	DT	denotes	This
T9740	19120-19126	NN	denotes	result
T9741	19127-19135	VB	denotes	suggests
T9742	19136-19144	RB	denotes	strongly
T9743	19145-19149	IN	denotes	that
T9744	19150-19158	NN	denotes	perforin
T9745	19159-19169	NN	denotes	expression
T9746	19170-19178	VB	denotes	requires
T9747	19179-19184	NN	denotes	Runx3
T9748	19185-19188	CC	denotes	and
T9749	19189-19194	NN	denotes	Eomes
T21280	19206-19211	NN	denotes	Runx3
T21281	19212-19220	VB	denotes	controls
T21282	19221-19226	NN	denotes	Eomes
T21283	19226-19227	-COMMA-	denotes	,
T21284	19228-19236	NN	denotes	perforin
T21285	19236-19237	-COMMA-	denotes	,
T21286	19238-19246	NN	denotes	granzyme
T21287	19247-19248	NN	denotes	B
T21288	19248-19249	-COMMA-	denotes	,
T21289	19250-19253	CC	denotes	and
T21290	19254-19259	NN	denotes	IFN-γ
T21291	19260-19270	NN	denotes	expression
T21292	19271-19273	IN	denotes	in
T21293	19274-19282	NN	denotes	effector
T21294	19283-19287	NN	denotes	CTLs
T21295	19289-19297	NN	denotes	Runx3+/+
T21296	19298-19300	CC	denotes	or
T21297	19301-19309	NN	denotes	Runx3−/−
T21298	19310-19314	JJ	denotes	CD8+
T21299	19315-19316	NN	denotes	T
T21300	19317-19322	NN	denotes	cells
T21301	19323-19327	VB	denotes	were
T21302	19328-19337	VB	denotes	activated
T21303	19338-19341	CC	denotes	and
T21304	19342-19352	VB	denotes	transduced
T21305	19353-19357	IN	denotes	with
T21306	19358-19370	NN	denotes	retroviruses
T21307	19371-19378	VB	denotes	bearing
T21308	19379-19381	DT	denotes	an
T21309	19382-19387	JJ	denotes	empty
T21310	19388-19396	NN	denotes	IRES-GFP
T21311	19397-19405	NN	denotes	cassette
T21312	19406-19407	-LRB-	denotes	(
T21313	19407-19410	NN	denotes	GFP
T21314	19410-19411	-RRB-	denotes	)
T21315	19412-19414	CC	denotes	or
T21316	19415-19419	RB	denotes	also
T21317	19420-19428	VB	denotes	encoding
T21318	19429-19439	NN	denotes	Eomes-VP16
T21319	19440-19441	-LRB-	denotes	(
T21320	19441-19448	NN	denotes	Eo-VP16
T21321	19448-19449	-RRB-	denotes	)
T21322	19450-19452	CC	denotes	or
T21323	19453-19462	NN	denotes	Myc-Runx3
T21324	19463-19464	-LRB-	denotes	(
T21325	19464-19469	NN	denotes	Runx3
T21326	19469-19470	-RRB-	denotes	)
T21327	19472-19475	DT	denotes	The
T21328	19476-19485	NN	denotes	frequency
T21329	19486-19488	IN	denotes	of
T21330	19489-19499	VB	denotes	transduced
T21331	19500-19505	NN	denotes	cells
T21332	19506-19508	IN	denotes	in
T21333	19509-19512	DT	denotes	the
T21334	19513-19521	NN	denotes	cultures
T21335	19522-19525	VB	denotes	was
T21336	19526-19536	JJ	denotes	equivalent
T21337	19537-19540	IN	denotes	for
T21338	19541-19544	DT	denotes	all
T21339	19545-19555	NN	denotes	constructs
T21340	19556-19557	-LRB-	denotes	(
T21341	19557-19563	CD	denotes	∼75–90
T21342	19563-19564	NN	denotes	%
T21343	19565-19569	NN	denotes	GFP+
T21344	19570-19575	NN	denotes	cells
T21345	19575-19576	-COLON-	denotes	;
T21346	19577-19580	RB	denotes	not
T21347	19581-19589	VB	denotes	depicted
T21348	19589-19590	-RRB-	denotes	)
T21349	19590-19591	-LRB-	denotes	.
T21350	19592-19593	NN	denotes	(
T21351	19593-19594	-RRB-	denotes	A
T21352	19594-19601	NN	denotes	) Prote
T21353	19601-19611	NN	denotes	in express
T21354	19611-19613	IN	denotes	io
T21355	19613-19623	JJ	denotes	n in whole
T21356	19623-19631	NN	denotes	-cell ex
T21357	19631-19632	-LRB-	denotes	t
T21358	19632-19635	NN	denotes	rac
T21359	19635-19636	CD	denotes	t
T21360	19636-19637	-RRB-	denotes	s
T21361	19638-19641	VB	denotes	(da
T21362	19641-19649	VB	denotes	y 6) was
T21363	19650-19652	IN	denotes	an
T21364	19652-19666	NN	denotes	alyzed by immu
T21365	19678-19692	NN	denotes	Overexpression
T21366	19693-19695	IN	denotes	of
T21367	19696-19706	NN	denotes	Eomes-VP16
T21368	19707-19710	MD	denotes	can
T21369	19710-19713	RB	denotes	not
T21370	19714-19716	VB	denotes	be
T21371	19717-19725	VB	denotes	detected
T21372	19726-19730	IN	denotes	with
T21373	19731-19734	DT	denotes	the
T21374	19735-19740	NN	denotes	Eomes
T21375	19741-19749	NN	denotes	antibody
T21376	19749-19750	-COMMA-	denotes	,
T21377	19751-19753	IN	denotes	as
T21378	19754-19757	DT	denotes	the
T21379	19758-19768	JJ	denotes	C-terminal
T21380	19769-19776	NN	denotes	epitope
T21381	19777-19779	VB	denotes	is
T21382	19780-19786	IN	denotes	within
T21383	19787-19790	DT	denotes	the
T21384	19791-19797	NN	denotes	region
T21385	19798-19802	WDT	denotes	that
T21386	19803-19806	VB	denotes	has
T21387	19807-19811	VB	denotes	been
T21388	19812-19820	VB	denotes	replaced
T21389	19821-19825	IN	denotes	with
T21390	19826-19829	DT	denotes	the
T21391	19830-19834	NN	denotes	VP16
T21392	19835-19850	NN	denotes	transactivation
T21393	19851-19858	NN	denotes	domain.
T21394	19859-19860	-LRB-	denotes	(
T21395	19860-19861	NN	denotes	B
T21396	19861-19862	-RRB-	denotes	)
T21397	19863-19873	NN	denotes	Expression
T21398	19874-19876	IN	denotes	of
T21399	19877-19885	NN	denotes	granzyme
T21400	19886-19887	NN	denotes	B
T21401	19888-19891	CC	denotes	and
T21402	19892-19897	NN	denotes	IFN-γ
T21403	19898-19903	IN	denotes	after
T21404	19904-19911	NN	denotes	culture
T21405	19912-19915	IN	denotes	for
T21406	19916-19917	CD	denotes	6
T21407	19918-19919	NN	denotes	d
T21408	19920-19923	CC	denotes	and
T21409	19924-19937	NN	denotes	restimulation
T21410	19938-19941	IN	denotes	for
T21411	19942-19943	CD	denotes	4
T21412	19944-19945	NN	denotes	h
T21413	19946-19950	IN	denotes	with
T21414	19951-19954	NN	denotes	PMA
T21415	19955-19958	CC	denotes	and
T21416	19959-19968	NN	denotes	ionomycin
T21417	19969-19972	VB	denotes	was
T21418	19973-19983	VB	denotes	determined
T21419	19984-19986	IN	denotes	by
T21420	19987-20000	JJ	denotes	intracellular
T21421	20001-20009	NN	denotes	staining
T21422	20011-20014	DT	denotes	The
T21423	20015-20025	NN	denotes	percentage
T21424	20026-20028	IN	denotes	of
T21425	20029-20039	RB	denotes	positively
T21426	20040-20047	VB	denotes	stained
T21427	20048-20053	NN	denotes	cells
T21428	20054-20056	VB	denotes	is
T21429	20057-20062	VB	denotes	shown
T21430	20063-20068	IN	denotes	above
T21431	20069-20072	DT	denotes	the
T21432	20073-20077	NN	denotes	gate
T21433	20077-20078	-COLON-	denotes	;
T21434	20079-20082	DT	denotes	the
T21435	20083-20087	JJ	denotes	mean
T21436	20088-20100	NN	denotes	fluorescence
T21437	20101-20110	NN	denotes	intensity
T21438	20111-20112	-LRB-	denotes	(
T21439	20112-20115	NN	denotes	MFI
T21440	20115-20116	-RRB-	denotes	)
T21441	20117-20119	IN	denotes	of
T21442	20120-20128	NN	denotes	granzyme
T21443	20129-20130	NN	denotes	B
T21444	20131-20139	NN	denotes	staining
T21445	20140-20143	IN	denotes	for
T21446	20144-20147	DT	denotes	the
T21447	20148-20153	JJ	denotes	total
T21448	20154-20164	NN	denotes	population
T21449	20165-20167	VB	denotes	is
T21450	20168-20173	VB	denotes	shown
T21451	20174-20179	IN	denotes	below
T21452	20180-20183	DT	denotes	the
T21453	20184-20188	NN	denotes	gate
T21454	20190-20193	DT	denotes	The
T21455	20194-20202	JJ	denotes	vertical
T21456	20203-20207	JJ	denotes	gray
T21457	20208-20213	NN	denotes	lines
T21458	20214-20222	VB	denotes	indicate
T21459	20223-20226	DT	denotes	the
T21460	20227-20230	NN	denotes	MFI
T21461	20231-20234	IN	denotes	for
T21462	20235-20237	JJ	denotes	WT
T21463	20238-20242	NN	denotes	GFP+
T21464	20243-20248	NN	denotes	cells
T21465	20250-20257	NN	denotes	Results
T21466	20258-20261	VB	denotes	are
T21467	20262-20276	JJ	denotes	representative
T21468	20277-20279	IN	denotes	of
T21469	20280-20282	IN	denotes	at
T21470	20283-20288	JJ	denotes	least
T21471	20289-20292	CD	denotes	two
T21472	20293-20304	JJ	denotes	independent
T21473	20305-20317	NN	denotes	experiments.
T21474	20318-20319	-LRB-	denotes	(
T21475	20319-20320	NN	denotes	C
T21476	20320-20321	-RRB-	denotes	)
T21477	20322-20331	JJ	denotes	Schematic
T21478	20332-20339	NN	denotes	diagram
T21479	20340-20342	IN	denotes	of
T21480	20343-20346	DT	denotes	the
T21481	20347-20362	JJ	denotes	transcriptional
T21482	20363-20370	NN	denotes	network
T21483	20371-20380	VB	denotes	involving
T21484	20381-20386	NN	denotes	Runx3
T21485	20387-20390	CC	denotes	and
T21486	20391-20396	NN	denotes	T-box
T21487	20397-20404	NN	denotes	factors
T21488	20406-20411	NN	denotes	T-bet
T21489	20412-20414	VB	denotes	is
T21490	20415-20422	VB	denotes	induced
T21491	20423-20425	IN	denotes	by
T21492	20426-20429	NN	denotes	TCR
T21493	20430-20437	NN	denotes	signals
T21494	20438-20441	CC	denotes	and
T21495	20442-20444	VB	denotes	is
T21496	20445-20454	JJ	denotes	essential
T21497	20455-20458	IN	denotes	for
T21498	20459-20464	JJ	denotes	early
T21499	20465-20470	NN	denotes	IFN-γ
T21500	20471-20481	NN	denotes	expression
T21501	20483-20488	NN	denotes	Runx3
T21502	20489-20491	VB	denotes	is
T21503	20492-20499	JJ	denotes	present
T21504	20500-20502	IN	denotes	in
T21505	20503-20508	JJ	denotes	naive
T21506	20509-20513	JJ	denotes	CD8+
T21507	20514-20515	NN	denotes	T
T21508	20516-20521	NN	denotes	cells
T21509	20522-20525	CC	denotes	and
T21510	20526-20535	VB	denotes	represses
T21511	20536-20541	NN	denotes	Runx1
T21512	20542-20545	CC	denotes	and
T21513	20546-20553	VB	denotes	induces
T21514	20554-20559	NN	denotes	Eomes
T21515	20559-20560	-COMMA-	denotes	,
T21516	20561-20569	NN	denotes	perforin
T21517	20569-20570	-COMMA-	denotes	,
T21518	20571-20579	NN	denotes	granzyme
T21519	20580-20581	NN	denotes	B
T21520	20581-20582	-COMMA-	denotes	,
T21521	20583-20586	CC	denotes	and
T21522	20587-20592	NN	denotes	IFN-γ
T21523	20593-20603	NN	denotes	expression
T21524	20605-20610	NN	denotes	Eomes
T21525	20611-20614	MD	denotes	may
T21526	20615-20626	VB	denotes	participate
T21527	20627-20629	IN	denotes	in
T21528	20630-20640	VB	denotes	sustaining
T21529	20641-20645	JJ	denotes	late
T21530	20646-20651	NN	denotes	IFN-γ
T21531	20652-20662	NN	denotes	expression
T21532	20662-20663	-COMMA-	denotes	,
T21533	20664-20671	IN	denotes	whereas
T21534	20672-20677	NN	denotes	Runx3
T21535	20678-20681	CC	denotes	and
T21536	20682-20687	NN	denotes	Eomes
T21537	20688-20689	-LRB-	denotes	(
T21538	20689-20692	CC	denotes	but
T21539	20693-20696	RB	denotes	not
T21540	20697-20702	NN	denotes	T-bet
T21541	20702-20703	-RRB-	denotes	)
T21542	20704-20707	MD	denotes	may
T21543	20708-20717	VB	denotes	cooperate
T21544	20718-20720	TO	denotes	to
T21545	20721-20729	VB	denotes	activate
T21546	20730-20738	NN	denotes	perforin
T21547	20739-20749	NN	denotes	expression
T21548	20751-20754	DT	denotes	The
T21549	20755-20761	JJ	denotes	dotted
T21550	20762-20766	NN	denotes	line
T21551	20767-20776	VB	denotes	indicates
T21552	20777-20780	DT	denotes	the
T21553	20781-20788	JJ	denotes	partial
T21554	20789-20795	NN	denotes	effect
T21555	20796-20798	IN	denotes	of
T21556	20799-20804	JJ	denotes	T-bet
T21557	20805-20815	NN	denotes	deficiency
T21558	20816-20818	IN	denotes	on
T21559	20819-20823	NN	denotes	Gzmb
T21560	20824-20828	NN	denotes	mRNA
T21561	20829-20832	CC	denotes	but
T21562	20833-20836	RB	denotes	not
T21563	20837-20845	NN	denotes	granzyme
T21564	20846-20847	NN	denotes	B
T21565	20848-20855	NN	denotes	protein
T21566	20856-20866	NN	denotes	expression
T9750	20867-20869	IN	denotes	As
T9751	20870-20878	VB	denotes	expected
T9752	20879-20883	IN	denotes	from
T9753	20884-20889	PRP-DOLLAR-	denotes	their
T9754	20890-20896	NN	denotes	defect
T9755	20897-20899	IN	denotes	in
T9756	20900-20908	NN	denotes	perforin
T9757	20909-20912	CC	denotes	and
T9758	20913-20921	NN	denotes	granzyme
T9759	20922-20923	NN	denotes	B
T9760	20924-20934	NN	denotes	expression
T9761	20934-20935	-COMMA-	denotes	,
T9762	20936-20944	NN	denotes	Runx3−/−
T9763	20945-20949	JJ	denotes	CD8+
T9764	20950-20951	NN	denotes	T
T9765	20952-20957	NN	denotes	cells
T9766	20958-20964	VB	denotes	showed
T9767	20965-20974	JJ	denotes	defective
T9768	20975-20984	JJ	denotes	cytolytic
T9769	20985-20993	NN	denotes	activity
T9770	20994-20996	IN	denotes	in
T9771	20997-20998	DT	denotes	a
T9772	20999-21004	JJ	denotes	mixed
T9773	21005-21015	NN	denotes	lymphocyte
T9774	21016-21024	NN	denotes	reaction
T9775	21025-21026	-LRB-	denotes	(
T9776	21026-21028	CD	denotes	12
T9777	21028-21029	-RRB-	denotes	)
T9778	21031-21038	RB	denotes	However
T9779	21038-21039	-COMMA-	denotes	,
T9780	21040-21054	JJ	denotes	TCR-stimulated
T9781	21055-21063	NN	denotes	Runx3−/−
T9782	21064-21068	JJ	denotes	CD8+
T9783	21069-21074	NN	denotes	cells
T9784	21075-21079	VB	denotes	were
T9785	21080-21082	RB	denotes	as
T9786	21083-21092	JJ	denotes	effective
T9787	21093-21095	IN	denotes	as
T9788	21096-21098	JJ	denotes	WT
T9789	21099-21104	NN	denotes	cells
T9790	21105-21107	IN	denotes	in
T9791	21108-21115	VB	denotes	killing
T9792	21116-21121	NN	denotes	tumor
T9793	21122-21127	NN	denotes	cells
T9794	21128-21130	IN	denotes	in
T9795	21131-21132	DT	denotes	a
T9796	21133-21143	JJ	denotes	redirected
T9797	21144-21147	NN	denotes	CTL
T9798	21148-21153	NN	denotes	assay
T9799	21154-21155	-LRB-	denotes	(
T9800	21155-21157	CD	denotes	12
T9801	21157-21158	-RRB-	denotes	)
T9802	21160-21171	RB	denotes	Furthermore
T9803	21171-21172	-COMMA-	denotes	,
T9804	21173-21177	JJ	denotes	CD8+
T9805	21178-21183	NN	denotes	cells
T9806	21184-21188	IN	denotes	from
T9807	21189-21192	DT	denotes	the
T9808	21193-21203	JJ	denotes	peritoneal
T9809	21204-21210	NN	denotes	cavity
T9810	21211-21213	IN	denotes	of
T9811	21214-21222	NN	denotes	Runx3−/−
T9812	21223-21227	NN	denotes	mice
T9813	21228-21237	VB	denotes	immunized
T9814	21238-21242	IN	denotes	with
T9815	21243-21250	JJ	denotes	certain
T9816	21251-21256	NN	denotes	tumor
T9817	21257-21262	NN	denotes	cells
T9818	21263-21274	RB	denotes	effectively
T9819	21275-21281	VB	denotes	killed
T9820	21282-21287	DT	denotes	these
T9821	21288-21295	NN	denotes	targets
T9822	21296-21297	-LRB-	denotes	(
T9823	21297-21299	CD	denotes	13
T9824	21299-21300	-RRB-	denotes	)
T9825	21302-21311	RB	denotes	Therefore
T9826	21311-21312	-COMMA-	denotes	,
T9827	21313-21321	IN	denotes	although
T9828	21322-21332	NN	denotes	activation
T9829	21333-21335	IN	denotes	of
T9830	21336-21339	DT	denotes	the
T9831	21340-21357	NN	denotes	perforin/granzyme
T9832	21358-21359	NN	denotes	B
T9833	21360-21369	NN	denotes	machinery
T9834	21370-21372	VB	denotes	is
T9835	21373-21382	JJ	denotes	defective
T9836	21383-21385	IN	denotes	in
T9837	21386-21394	NN	denotes	Runx3−/−
T9838	21395-21399	JJ	denotes	CD8+
T9839	21400-21405	NN	denotes	cells
T9840	21405-21406	-COMMA-	denotes	,
T9841	21407-21412	DT	denotes	these
T9842	21413-21418	NN	denotes	cells
T9843	21419-21422	VB	denotes	are
T9844	21423-21426	RB	denotes	not
T9845	21427-21435	RB	denotes	entirely
T9846	21436-21442	JJ	denotes	devoid
T9847	21443-21445	IN	denotes	of
T9848	21446-21455	JJ	denotes	cytolytic
T9849	21456-21464	NN	denotes	activity
T9850	21465-21468	CC	denotes	and
T9851	21469-21474	MD	denotes	could
T9852	21475-21480	RB	denotes	still
T9853	21481-21492	RB	denotes	effectively
T9854	21493-21497	VB	denotes	kill
T9855	21498-21505	NN	denotes	targets
T9856	21505-21506	-COMMA-	denotes	,
T9857	21507-21515	RB	denotes	possibly
T9858	21516-21518	IN	denotes	by
T9859	21519-21530	JJ	denotes	alternative
T9860	21531-21541	NN	denotes	mechanisms
T9861	21542-21546	JJ	denotes	such
T9862	21547-21549	IN	denotes	as
T9863	21550-21553	DT	denotes	the
T9864	21554-21561	NN	denotes	Fas–Fas
T9865	21562-21568	NN	denotes	ligand
T9866	21569-21576	NN	denotes	pathway
T14019	25300-25310	NN	denotes	Antibodies
T14020	25311-25314	CC	denotes	and
T14021	25315-25323	NN	denotes	reagents
T14022	25325-25328	DT	denotes	The
T14023	25329-25338	JJ	denotes	following
T14024	25339-25349	NN	denotes	antibodies
T14025	25350-25354	VB	denotes	used
T14026	25355-25358	IN	denotes	for
T14027	25359-25372	JJ	denotes	intracellular
T14028	25373-25375	CC	denotes	or
T14029	25376-25383	NN	denotes	surface
T14030	25384-25390	NN	denotes	stains
T14031	25391-25395	VB	denotes	were
T14032	25396-25404	VB	denotes	obtained
T14033	25405-25409	IN	denotes	from
T14034	25410-25421	NN	denotes	eBioscience
T14035	25421-25422	-COLON-	denotes	:
T14036	25423-25432	NN	denotes	anti–IL-2
T14037	25432-25433	-COMMA-	denotes	,
T14038	25434-25444	JJ	denotes	anti–IFN-γ
T14039	25444-25445	-COMMA-	denotes	,
T14040	25446-25454	JJ	denotes	anti-TNF
T14041	25454-25455	-COMMA-	denotes	,
T14042	25456-25469	NN	denotes	anti–granzyme
T14043	25470-25471	NN	denotes	B
T14044	25471-25472	-COMMA-	denotes	,
T14045	25473-25481	NN	denotes	anti-CD8
T14046	25481-25482	-COMMA-	denotes	,
T14047	25483-25492	NN	denotes	anti-CD25
T14048	25492-25493	-COMMA-	denotes	,
T14049	25494-25497	CC	denotes	and
T14050	25498-25507	NN	denotes	anti-CD44
T14051	25509-25518	NN	denotes	Anti-CD69
T14052	25519-25522	VB	denotes	was
T14053	25523-25532	VB	denotes	purchased
T14054	25533-25537	IN	denotes	from
T14055	25538-25540	NN	denotes	BD
T14056	25542-25545	IN	denotes	For
T14057	25546-25550	NN	denotes	ChIP
T14058	25551-25562	NN	denotes	experiments
T14059	25562-25563	-COMMA-	denotes	,
T14060	25564-25567	DT	denotes	the
T14061	25568-25578	JJ	denotes	anti-Eomes
T14062	25579-25587	NN	denotes	antibody
T14063	25588-25591	VB	denotes	was
T14064	25592-25600	VB	denotes	obtained
T14065	25601-25605	IN	denotes	from
T14066	25606-25611	NN	denotes	Abcam
T14067	25612-25615	CC	denotes	and
T14068	25616-25619	DT	denotes	the
T14069	25620-25630	JJ	denotes	anti-Runx3
T14070	25631-25639	NN	denotes	antibody
T14071	25640-25643	VB	denotes	was
T14072	25644-25652	VB	denotes	produced
T14073	25653-25655	IN	denotes	by
T14074	25656-25659	DT	denotes	the
T14075	25660-25666	NNP	denotes	Groner
T14076	25667-25677	NN	denotes	laboratory
T14077	25679-25682	DT	denotes	The
T14078	25683-25692	VB	denotes	following
T14079	25693-25703	NN	denotes	antibodies
T14080	25704-25708	VB	denotes	were
T14081	25709-25713	VB	denotes	used
T14082	25714-25717	IN	denotes	for
T14083	25718-25732	NN	denotes	immunoblotting
T14084	25732-25733	-COLON-	denotes	:
T14085	25734-25746	NN	denotes	antiperforin
T14086	25747-25748	-LRB-	denotes	(
T14087	25748-25753	NN	denotes	Abcam
T14088	25753-25754	-RRB-	denotes	)
T14089	25754-25755	-COMMA-	denotes	,
T14090	25756-25766	JJ	denotes	anti-Eomes
T14091	25767-25768	-LRB-	denotes	(
T14092	25768-25773	NN	denotes	Abcam
T14093	25773-25774	-RRB-	denotes	)
T14094	25774-25775	-COMMA-	denotes	,
T14095	25776-25779	CC	denotes	and
T14096	25780-25791	NN	denotes	anti–Pol-II
T14097	25792-25793	-LRB-	denotes	(
T14098	25793-25798	NNP	denotes	Santa
T14099	25799-25803	NNP	denotes	Cruz
T14100	25804-25817	NNP	denotes	Biotechnology
T14101	25817-25818	-COMMA-	denotes	,
T14102	25819-25823	NNP	denotes	Inc.
T14103	25823-25824	-RRB-	denotes	)
T14104	25826-25829	DT	denotes	The
T14105	25830-25835	JJ	denotes	T-bet
T14106	25836-25844	NN	denotes	antibody
T14107	25845-25848	VB	denotes	was
T14108	25849-25857	VB	denotes	provided
T14109	25858-25860	IN	denotes	by
T14110	25861-25863	NNP	denotes	L.
T14111	25864-25872	NNP	denotes	Glimcher
T14112	25873-25874	-LRB-	denotes	(
T14113	25874-25881	NNP	denotes	Harvard
T14114	25882-25888	NNP	denotes	School
T14115	25889-25891	IN	denotes	of
T14116	25892-25898	NNP	denotes	Public
T14117	25899-25905	NNP	denotes	Health
T14118	25905-25906	-COMMA-	denotes	,
T14119	25907-25913	NNP	denotes	Boston
T14120	25913-25914	-COMMA-	denotes	,
T14121	25915-25917	NNP	denotes	MA
T14122	25917-25918	-RRB-	denotes	)
T14123	25920-25923	DT	denotes	The
T14124	25924-25933	VB	denotes	following
T14125	25934-25942	NN	denotes	reagents
T14126	25943-25947	VB	denotes	were
T14127	25948-25952	VB	denotes	used
T14128	25953-25956	IN	denotes	for
T14129	25957-25960	DT	denotes	the
T14130	25961-25972	NN	denotes	experiments
T14131	25973-25982	VB	denotes	presented
T14132	25983-25985	IN	denotes	in
T14133	25986-25990	DT	denotes	this
T14134	25991-25997	NN	denotes	report
T14135	25997-25998	-COLON-	denotes	:
T14136	25999-26006	NN	denotes	Annexin
T14137	26007-26013	NNP	denotes	V–FITC
T14138	26014-26023	NNP	denotes	Apoptosis
T14139	26024-26033	NN	denotes	Detection
T14140	26034-26037	NN	denotes	Kit
T14141	26038-26039	-LRB-	denotes	(
T14142	26039-26041	NN	denotes	BD
T14143	26041-26042	-RRB-	denotes	)
T14144	26042-26043	-COMMA-	denotes	,
T14145	26044-26047	NN	denotes	CD8
T14146	26048-26056	JJ	denotes	Negative
T14147	26057-26066	NN	denotes	Isolation
T14148	26067-26070	NN	denotes	Kit
T14149	26071-26072	-LRB-	denotes	(
T14150	26072-26082	NN	denotes	Invitrogen
T14151	26082-26083	-RRB-	denotes	)
T14152	26083-26084	-COMMA-	denotes	,
T14153	26085-26088	NN	denotes	CD8
T14154	26089-26099	NN	denotes	MicroBeads
T14155	26100-26101	-LRB-	denotes	(
T14156	26101-26109	NNP	denotes	Miltenyi
T14157	26110-26116	NNP	denotes	Biotec
T14158	26116-26117	-RRB-	denotes	)
T14159	26117-26118	-COMMA-	denotes	,
T14160	26119-26122	CC	denotes	and
T14161	26123-26127	NNP	denotes	SYBR
T14162	26128-26133	NNP	denotes	Green
T14163	26134-26137	NN	denotes	PCR
T14164	26138-26142	NNP	denotes	Core
T14165	26143-26151	NNP	denotes	Reagents
T14166	26152-26153	-LRB-	denotes	(
T14167	26153-26160	NNP	denotes	Applied
T14168	26161-26171	NNP	denotes	Biosystems
T14169	26171-26172	-RRB-	denotes	)
T14170	26174-26177	DT	denotes	The
T14171	26178-26182	NN	denotes	Gp33
T14172	26183-26190	NN	denotes	peptide
T14173	26191-26192	-LRB-	denotes	(
T14174	26192-26201	NN	denotes	KAVYNFATC
T14175	26201-26202	-RRB-	denotes	)
T14176	26203-26206	VB	denotes	was
T14177	26207-26218	VB	denotes	synthesized
T14178	26219-26221	IN	denotes	by
T14179	26222-26225	DT	denotes	the
T14180	26226-26231	NNP	denotes	Tufts
T14181	26232-26242	NNP	denotes	University
T14182	26243-26247	NNP	denotes	Core
T14183	26248-26256	NNP	denotes	Facility
T14184	26256-26257	-COMMA-	denotes	,
T14185	26258-26261	CC	denotes	and
T14186	26262-26264	CD	denotes	10
T14187	26265-26267	NN	denotes	mM
T14188	26268-26270	IN	denotes	of
T14189	26271-26276	NN	denotes	stock
T14190	26277-26286	NN	denotes	solutions
T14191	26287-26290	VB	denotes	was
T14192	26291-26299	VB	denotes	prepared
T14193	26300-26302	IN	denotes	in
T14194	26303-26307	NN	denotes	DMSO
T14607	26310-26319	NN	denotes	Isolation
T14608	26320-26323	CC	denotes	and
T14609	26324-26331	NN	denotes	culture
T14610	26332-26334	IN	denotes	of
T14611	26335-26342	JJ	denotes	primary
T14612	26343-26347	JJ	denotes	CD8+
T14613	26348-26349	NN	denotes	T
T14614	26350-26355	NN	denotes	cells
T14615	26357-26361	JJ	denotes	CD8+
T14616	26362-26363	NN	denotes	T
T14617	26364-26369	NN	denotes	cells
T14618	26370-26374	IN	denotes	from
T14619	26375-26385	JJ	denotes	4–8-wk-old
T14620	26386-26393	NN	denotes	Tcra−/−
T14621	26394-26395	NN	denotes	×
T14622	26396-26399	NN	denotes	P14
T14623	26400-26403	NN	denotes	TCR
T14624	26404-26414	JJ	denotes	transgenic
T14625	26415-26416	-LRB-	denotes	(
T14626	26416-26423	JJ	denotes	Taconic
T14627	26423-26424	-RRB-	denotes	)
T14628	26424-26425	-COMMA-	denotes	,
T14629	26426-26434	NN	denotes	C57BL/6J
T14630	26435-26437	NNP	denotes	WT
T14631	26437-26438	-COMMA-	denotes	,
T14632	26439-26441	CC	denotes	or
T14633	26442-26450	NN	denotes	Tbx21−/−
T14634	26451-26452	-LRB-	denotes	(
T14635	26452-26455	DT	denotes	The
T14636	26456-26463	NNP	denotes	Jackson
T14637	26464-26474	NNP	denotes	Laboratory
T14638	26474-26475	-RRB-	denotes	)
T14639	26476-26480	NN	denotes	mice
T14640	26481-26485	VB	denotes	were
T14641	26486-26494	VB	denotes	purified
T14642	26495-26496	-LRB-	denotes	(
T14643	26496-26497	SYM	denotes	>
T14644	26497-26499	CD	denotes	95
T14645	26499-26500	NN	denotes	%
T14646	26501-26507	NN	denotes	purity
T14647	26507-26508	-RRB-	denotes	)
T14648	26509-26511	IN	denotes	by
T14649	26512-26520	JJ	denotes	negative
T14650	26521-26530	NN	denotes	selection
T14651	26531-26532	-LRB-	denotes	(
T14652	26532-26542	NN	denotes	Invitrogen
T14653	26542-26543	-RRB-	denotes	)
T14654	26544-26548	IN	denotes	from
T14655	26549-26555	VB	denotes	pooled
T14656	26556-26562	NN	denotes	spleen
T14657	26563-26566	CC	denotes	and
T14658	26567-26572	NN	denotes	lymph
T14659	26573-26577	NN	denotes	node
T14660	26578-26583	NN	denotes	cells
T14661	26585-26589	JJ	denotes	CD8+
T14662	26590-26591	NN	denotes	T
T14663	26592-26597	NN	denotes	cells
T14664	26598-26602	IN	denotes	from
T14665	26603-26611	NN	denotes	Runx3−/−
T14666	26612-26616	NN	denotes	mice
T14667	26617-26619	IN	denotes	on
T14668	26620-26623	DT	denotes	the
T14669	26624-26627	NN	denotes	ICR
T14670	26628-26638	NN	denotes	background
T14671	26639-26643	VB	denotes	were
T14672	26644-26652	VB	denotes	purified
T14673	26653-26655	IN	denotes	by
T14674	26656-26664	JJ	denotes	positive
T14675	26665-26674	NN	denotes	selection
T14676	26675-26676	-LRB-	denotes	(
T14677	26676-26684	NNP	denotes	Miltenyi
T14678	26685-26691	NNP	denotes	Biotec
T14679	26691-26692	-RRB-	denotes	)
T14680	26694-26697	DT	denotes	All
T14681	26698-26702	NN	denotes	mice
T14682	26703-26707	VB	denotes	were
T14683	26708-26718	VB	denotes	maintained
T14684	26719-26721	IN	denotes	in
T14685	26722-26730	JJ	denotes	specific
T14686	26731-26744	JJ	denotes	pathogen-free
T14687	26745-26752	NN	denotes	barrier
T14688	26753-26763	NN	denotes	facilities
T14689	26764-26767	CC	denotes	and
T14690	26768-26772	VB	denotes	used
T14691	26773-26782	VB	denotes	according
T14692	26783-26785	TO	denotes	to
T14693	26786-26795	NN	denotes	protocols
T14694	26796-26804	VB	denotes	approved
T14695	26805-26807	IN	denotes	by
T14696	26808-26811	DT	denotes	the
T14697	26812-26818	NNP	denotes	Immune
T14698	26819-26826	NNP	denotes	Disease
T14699	26827-26836	NNP	denotes	Institute
T14700	26837-26840	CC	denotes	and
T14701	26841-26844	DT	denotes	the
T14702	26845-26852	NNP	denotes	Harvard
T14703	26853-26860	NNP	denotes	Medical
T14704	26861-26867	NNP	denotes	School
T14705	26868-26874	NNP	denotes	Animal
T14706	26875-26879	NNP	denotes	Care
T14707	26880-26883	CC	denotes	and
T14708	26884-26887	NNP	denotes	Use
T14709	26888-26899	NNP	denotes	Committees.
T14710	26900-26903	IN	denotes	For
T14711	26904-26915	NN	denotes	stimulation
T14712	26915-26916	-COMMA-	denotes	,
T14713	26917-26925	VB	denotes	purified
T14714	26926-26930	JJ	denotes	CD8+
T14715	26931-26932	NN	denotes	T
T14716	26933-26938	NN	denotes	cells
T14717	26939-26943	VB	denotes	were
T14718	26944-26952	VB	denotes	cultured
T14719	26953-26955	IN	denotes	at
T14720	26956-26959	CD	denotes	106
T14721	26960-26968	NN	denotes	cells/ml
T14722	26969-26970	-LRB-	denotes	(
T14723	26970-26972	CD	denotes	10
T14724	26973-26975	NN	denotes	ml
T14725	26975-26976	-RRB-	denotes	)
T14726	26977-26979	IN	denotes	in
T14727	26980-26983	NN	denotes	T25
T14728	26984-26990	NN	denotes	flasks
T14729	26991-26997	VB	denotes	coated
T14730	26998-27002	IN	denotes	with
T14731	27003-27004	CD	denotes	1
T14732	27005-27010	NN	denotes	μg/ml
T14733	27011-27015	DT	denotes	each
T14734	27016-27018	IN	denotes	of
T14735	27019-27027	NN	denotes	anti-CD3
T14736	27028-27029	-LRB-	denotes	(
T14737	27029-27034	NN	denotes	clone
T14738	27035-27039	NN	denotes	2C11
T14739	27039-27040	-RRB-	denotes	)
T14740	27041-27044	CC	denotes	and
T14741	27045-27054	JJ	denotes	anti-CD28
T14742	27055-27056	-LRB-	denotes	(
T14743	27056-27061	NN	denotes	clone
T14744	27062-27067	CD	denotes	37.51
T14745	27067-27068	-RRB-	denotes	)
T14746	27069-27071	IN	denotes	by
T14747	27072-27084	NN	denotes	pretreatment
T14748	27085-27089	IN	denotes	with
T14749	27090-27093	CD	denotes	300
T14750	27094-27099	NN	denotes	μg/ml
T14751	27100-27104	NN	denotes	goat
T14752	27105-27117	NN	denotes	anti–hamster
T14753	27118-27121	NN	denotes	IgG
T14754	27123-27128	IN	denotes	After
T14755	27129-27131	CD	denotes	48
T14756	27132-27133	NN	denotes	h
T14757	27133-27134	-COMMA-	denotes	,
T14758	27135-27140	NN	denotes	cells
T14759	27141-27145	VB	denotes	were
T14760	27146-27153	VB	denotes	removed
T14761	27154-27158	IN	denotes	from
T14762	27159-27162	DT	denotes	the
T14763	27163-27166	NN	denotes	TCR
T14764	27167-27178	NN	denotes	stimulation
T14765	27179-27182	CC	denotes	and
T14766	27183-27193	VB	denotes	recultured
T14767	27194-27196	IN	denotes	at
T14768	27197-27198	DT	denotes	a
T14769	27199-27212	NN	denotes	concentration
T14770	27213-27215	IN	denotes	of
T14771	27216-27217	CD	denotes	5
T14772	27218-27219	IN	denotes	×
T14773	27220-27223	CD	denotes	105
T14774	27224-27232	NN	denotes	cells/ml
T14775	27233-27235	IN	denotes	in
T14776	27236-27241	NN	denotes	media
T14777	27242-27254	VB	denotes	supplemented
T14778	27255-27259	IN	denotes	with
T14779	27260-27263	CD	denotes	100
T14780	27264-27268	NN	denotes	U/ml
T14781	27269-27275	NN	denotes	rhIL-2
T14782	27277-27282	DT	denotes	Every
T14783	27283-27285	CD	denotes	24
T14784	27286-27287	NN	denotes	h
T14785	27287-27288	-COMMA-	denotes	,
T14786	27289-27295	JJ	denotes	viable
T14787	27296-27301	NN	denotes	cells
T14788	27302-27306	VB	denotes	were
T14789	27307-27314	VB	denotes	counted
T14790	27315-27318	CC	denotes	and
T14791	27319-27329	VB	denotes	readjusted
T14792	27330-27332	TO	denotes	to
T14793	27333-27334	CD	denotes	5
T14794	27335-27336	IN	denotes	×
T14795	27337-27340	CD	denotes	105
T14796	27341-27349	NN	denotes	cells/ml
T14797	27350-27354	IN	denotes	with
T14798	27355-27360	JJ	denotes	fresh
T14799	27361-27366	NN	denotes	media
T14800	27367-27377	VB	denotes	containing
T14801	27378-27381	DT	denotes	the
T14802	27382-27395	VB	denotes	corresponding
T14803	27396-27402	NN	denotes	amount
T14804	27403-27405	IN	denotes	of
T14805	27406-27412	NN	denotes	rhIL-2
T15411	27415-27424	NN	denotes	Isolation
T15412	27425-27427	IN	denotes	of
T15413	27428-27432	JJ	denotes	CD8+
T15414	27433-27434	NN	denotes	T
T15415	27435-27440	NN	denotes	cells
T15416	27441-27445	IN	denotes	from
T15417	27446-27454	NN	denotes	Runx3−/−
T15418	27455-27459	NN	denotes	mice
T15419	27461-27476	JJ	denotes	Runx3-deficient
T15420	27477-27478	NN	denotes	T
T15421	27479-27484	NN	denotes	cells
T15422	27485-27489	VB	denotes	fail
T15423	27490-27492	TO	denotes	to
T15424	27493-27500	NN	denotes	silence
T15425	27501-27504	NN	denotes	CD4
T15426	27505-27515	NN	denotes	expression
T15427	27516-27524	RB	denotes	normally
T15428	27525-27526	-LRB-	denotes	(
T15429	27526-27530	NNP	denotes	Fig.
T15430	27531-27533	NN	denotes	S1
T15431	27533-27534	-RRB-	denotes	)
T15432	27535-27536	-LRB-	denotes	(
T15433	27536-27538	CD	denotes	12
T15434	27538-27539	-COMMA-	denotes	,
T15435	27540-27542	CD	denotes	13
T15436	27542-27543	-RRB-	denotes	)
T15437	27545-27547	PRP	denotes	We
T15438	27548-27557	RB	denotes	therefore
T15439	27558-27565	RB	denotes	further
T15440	27566-27578	VB	denotes	fractionated
T15441	27579-27582	DT	denotes	the
T15442	27583-27593	RB	denotes	positively
T15443	27594-27602	VB	denotes	selected
T15444	27603-27607	JJ	denotes	CD8+
T15445	27608-27609	NN	denotes	T
T15446	27610-27615	NN	denotes	cells
T15447	27616-27620	IN	denotes	from
T15448	27621-27626	NN	denotes	Runx3
T15449	27627-27629	NN	denotes	KO
T15450	27630-27634	NN	denotes	mice
T15451	27635-27639	IN	denotes	into
T15452	27640-27648	NN	denotes	CD8+CD4−
T15453	27649-27651	NN	denotes	SP
T15454	27652-27654	CC	denotes	or
T15455	27655-27663	JJ	denotes	CD8+CD4+
T15456	27664-27666	NN	denotes	DP
T15457	27667-27672	NN	denotes	cells
T15458	27673-27675	IN	denotes	by
T15459	27676-27686	NN	denotes	separation
T15460	27687-27692	VB	denotes	using
T15461	27693-27701	JJ	denotes	anti-CD4
T15462	27702-27710	JJ	denotes	magnetic
T15463	27711-27716	NN	denotes	beads
T15464	27718-27722	DT	denotes	This
T15465	27723-27730	VB	denotes	yielded
T15466	27731-27732	DT	denotes	a
T15467	27733-27738	NN	denotes	Runx3
T15468	27739-27741	NN	denotes	KO
T15469	27742-27744	NN	denotes	SP
T15470	27745-27755	VB	denotes	“enriched”
T15471	27756-27766	NN	denotes	population
T15472	27767-27771	WDT	denotes	that
T15473	27772-27781	VB	denotes	contained
T15474	27782-27784	CD	denotes	75
T15475	27784-27785	NN	denotes	%
T15476	27786-27794	NN	denotes	CD8+CD4−
T15477	27795-27800	NN	denotes	cells
T15478	27801-27804	CC	denotes	and
T15479	27805-27806	DT	denotes	a
T15480	27807-27809	NN	denotes	KO
T15481	27810-27812	NN	denotes	DP
T15482	27813-27821	VB	denotes	enriched
T15483	27822-27832	NN	denotes	population
T15484	27833-27837	WDT	denotes	that
T15485	27838-27847	VB	denotes	contained
T15486	27848-27850	CD	denotes	85
T15487	27850-27851	NN	denotes	%
T15488	27852-27860	JJ	denotes	CD8+CD4+
T15489	27861-27866	NN	denotes	cells
T15490	27867-27868	-LRB-	denotes	(
T15491	27868-27872	NN	denotes	Fig.
T15492	27873-27875	NN	denotes	S1
T15493	27875-27876	-RRB-	denotes	)
T15494	27878-27881	DT	denotes	The
T15495	27882-27887	NN	denotes	cells
T15496	27888-27892	VB	denotes	were
T15497	27893-27903	VB	denotes	stimulated
T15498	27904-27908	IN	denotes	with
T15499	27909-27918	JJ	denotes	anti-CD3+
T15500	27919-27928	NN	denotes	anti-CD28
T15501	27929-27932	IN	denotes	for
T15502	27933-27934	CD	denotes	2
T15503	27935-27936	NN	denotes	d
T15504	27937-27943	IN	denotes	before
T15505	27944-27952	VB	denotes	removing
T15506	27953-27957	PRP	denotes	them
T15507	27958-27962	IN	denotes	from
T15508	27963-27966	DT	denotes	the
T15509	27967-27970	NN	denotes	TCR
T15510	27971-27979	NN	denotes	stimulus
T15511	27980-27983	CC	denotes	and
T15512	27984-27993	VB	denotes	culturing
T15513	27994-27998	PRP	denotes	them
T15514	27999-28001	IN	denotes	in
T15515	28002-28007	NN	denotes	media
T15516	28008-28018	VB	denotes	containing
T15517	28019-28022	CD	denotes	100
T15518	28023-28027	NN	denotes	U/ml
T15519	28028-28032	NN	denotes	IL-2
T15520	28034-28036	IN	denotes	As
T15521	28037-28047	RB	denotes	previously
T15522	28048-28056	VB	denotes	reported
T15523	28056-28057	-COMMA-	denotes	,
T15524	28058-28069	JJ	denotes	TCR-induced
T15525	28070-28083	NN	denotes	proliferation
T15526	28084-28086	IN	denotes	of
T15527	28087-28095	NN	denotes	Runx3−/−
T15528	28096-28100	JJ	denotes	CD8+
T15529	28101-28102	NN	denotes	T
T15530	28103-28108	NN	denotes	cells
T15531	28109-28112	VB	denotes	was
T15532	28113-28121	RB	denotes	severely
T15533	28122-28130	JJ	denotes	impaired
T15534	28130-28131	-COMMA-	denotes	,
T15535	28132-28144	JJ	denotes	irrespective
T15536	28145-28147	IN	denotes	of
T15537	28148-28151	NN	denotes	CD4
T15538	28152-28162	NN	denotes	expression
T15539	28163-28164	-LRB-	denotes	(
T15540	28164-28168	NN	denotes	Fig.
T15541	28169-28171	NN	denotes	S1
T15542	28171-28172	-RRB-	denotes	)
T15543	28173-28174	-LRB-	denotes	(
T15544	28174-28176	CD	denotes	12
T15545	28176-28177	-COMMA-	denotes	,
T15546	28178-28180	CD	denotes	13
T15547	28180-28181	-RRB-	denotes	)
T15548	28183-28190	RB	denotes	However
T15549	28190-28191	-COMMA-	denotes	,
T15550	28192-28195	DT	denotes	the
T15551	28196-28204	NN	denotes	Runx3−/−
T15552	28205-28210	NN	denotes	cells
T15553	28211-28217	VB	denotes	showed
T15554	28218-28230	JJ	denotes	cell-surface
T15555	28231-28241	NN	denotes	expression
T15556	28242-28250	NN	denotes	patterns
T15557	28251-28261	JJ	denotes	indicative
T15558	28262-28264	IN	denotes	of
T15559	28265-28274	VB	denotes	activated
T15560	28275-28280	NN	denotes	cells
T15561	28280-28281	-COMMA-	denotes	,
T15562	28282-28291	VB	denotes	including
T15563	28292-28305	NN	denotes	up-regulation
T15564	28306-28308	IN	denotes	of
T15565	28309-28313	NN	denotes	CD25
T15566	28314-28317	CC	denotes	and
T15567	28318-28322	NN	denotes	CD69
T15568	28323-28324	-LRB-	denotes	(
T15569	28324-28328	NN	denotes	Fig.
T15570	28329-28331	NN	denotes	S1
T15571	28331-28332	-RRB-	denotes	)
T15572	28334-28336	IN	denotes	As
T15573	28337-28345	VB	denotes	expected
T15574	28346-28350	IN	denotes	from
T15575	28351-28356	PRP-DOLLAR-	denotes	their
T15576	28357-28364	NN	denotes	ability
T15577	28365-28367	TO	denotes	to
T15578	28368-28379	VB	denotes	up-regulate
T15579	28380-28384	NN	denotes	CD25
T15580	28384-28385	-COMMA-	denotes	,
T15581	28386-28394	NN	denotes	Runx3−/−
T15582	28395-28399	JJ	denotes	CD8+
T15583	28400-28401	NN	denotes	T
T15584	28402-28407	NN	denotes	cells
T15585	28408-28417	VB	denotes	responded
T15586	28418-28420	TO	denotes	to
T15587	28421-28425	NN	denotes	IL-2
T15588	28426-28441	NN	denotes	supplementation
T15589	28442-28447	IN	denotes	after
T15590	28448-28451	NN	denotes	day
T15591	28452-28453	CD	denotes	2
T15592	28454-28457	CC	denotes	and
T15593	28458-28469	RB	denotes	efficiently
T15594	28470-28478	VB	denotes	expanded
T15595	28479-28484	IN	denotes	until
T15596	28485-28488	NN	denotes	day
T15597	28489-28490	CD	denotes	6
T15598	28491-28493	IN	denotes	of
T15599	28494-28497	DT	denotes	the
T15600	28498-28505	NN	denotes	culture
T15601	28506-28512	NN	denotes	period
T15602	28512-28513	-COMMA-	denotes	,
T15603	28514-28520	IN	denotes	albeit
T15604	28521-28523	IN	denotes	at
T15605	28524-28530	JJ	denotes	slower
T15606	28531-28536	NN	denotes	rates
T15607	28537-28545	VB	denotes	compared
T15608	28546-28550	IN	denotes	with
T15609	28551-28553	JJ	denotes	WT
T15610	28554-28559	NN	denotes	cells
T15611	28560-28561	-LRB-	denotes	(
T15612	28561-28565	NN	denotes	Fig.
T15613	28566-28568	NN	denotes	S1
T15614	28568-28569	-RRB-	denotes	)
T15615	28571-28579	IN	denotes	Although
T15616	28580-28581	DT	denotes	a
T15617	28582-28590	NN	denotes	fraction
T15618	28591-28593	IN	denotes	of
T15619	28594-28597	DT	denotes	the
T15620	28598-28600	NN	denotes	KO
T15621	28601-28603	NN	denotes	DP
T15622	28604-28609	NN	denotes	cells
T15623	28610-28618	VB	denotes	silenced
T15624	28619-28622	NN	denotes	CD4
T15625	28623-28633	NN	denotes	expression
T15626	28634-28639	IN	denotes	after
T15627	28640-28650	NN	denotes	activation
T15628	28650-28651	-COMMA-	denotes	,
T15629	28652-28655	DT	denotes	the
T15630	28656-28661	NN	denotes	ratio
T15631	28662-28664	IN	denotes	of
T15632	28665-28670	NN	denotes	SP/DP
T15633	28671-28676	NN	denotes	cells
T15634	28677-28679	IN	denotes	in
T15635	28680-28684	DT	denotes	each
T15636	28685-28693	VB	denotes	enriched
T15637	28694-28704	NN	denotes	population
T15638	28705-28713	VB	denotes	remained
T15639	28714-28722	JJ	denotes	constant
T15640	28723-28733	RB	denotes	thereafter
T15641	28733-28734	-COMMA-	denotes	,
T15642	28735-28738	CC	denotes	and
T15643	28739-28741	PRP	denotes	we
T15644	28742-28745	VB	denotes	did
T15645	28746-28749	RB	denotes	not
T15646	28750-28757	VB	denotes	observe
T15647	28758-28761	DT	denotes	any
T15648	28762-28767	JJ	denotes	major
T15649	28768-28779	NN	denotes	differences
T15650	28780-28787	IN	denotes	between
T15651	28788-28793	DT	denotes	these
T15652	28794-28797	CD	denotes	two
T15653	28798-28809	NN	denotes	populations
T15654	28810-28820	IN	denotes	throughout
T15655	28821-28824	DT	denotes	the
T15656	28825-28832	NN	denotes	culture
T15657	28833-28839	NN	denotes	period
T15658	28839-28840	-COMMA-	denotes	,
T15659	28841-28851	VB	denotes	indicating
T15660	28852-28856	IN	denotes	that
T15661	28857-28859	IN	denotes	in
T15662	28860-28865	NN	denotes	terms
T15663	28866-28868	IN	denotes	of
T15664	28869-28877	NN	denotes	effector
T15665	28878-28881	NN	denotes	CTL
T15666	28882-28897	NN	denotes	differentiation
T15667	28898-28901	CC	denotes	and
T15668	28902-28907	IN	denotes	under
T15669	28908-28911	PRP-DOLLAR-	denotes	our
T15670	28912-28919	NN	denotes	culture
T15671	28920-28930	NN	denotes	conditions
T15672	28930-28931	-COMMA-	denotes	,
T15673	28932-28940	NN	denotes	Runx3−/−
T15674	28941-28945	JJ	denotes	CD8+
T15675	28946-28947	NN	denotes	T
T15676	28948-28953	NN	denotes	cells
T15677	28954-28958	WDT	denotes	that
T15678	28959-28963	RB	denotes	also
T15679	28964-28973	VB	denotes	coexpress
T15680	28974-28977	NN	denotes	CD4
T15681	28978-28981	VB	denotes	are
T15682	28982-28999	JJ	denotes	indistinguishable
T15683	29000-29004	IN	denotes	from
T15684	29005-29010	DT	denotes	those
T15685	29011-29015	WDT	denotes	that
T15686	29016-29018	VB	denotes	do
T15687	29019-29022	RB	denotes	not
T15688	29024-29027	DT	denotes	The
T15689	29028-29032	NN	denotes	data
T15690	29033-29042	VB	denotes	presented
T15691	29043-29045	IN	denotes	in
T15692	29046-29050	NNP	denotes	Fig.
T15693	29051-29053	NN	denotes	S2
T15694	29054-29057	VB	denotes	are
T15695	29058-29062	IN	denotes	from
T15696	29063-29068	NN	denotes	Runx3
T15697	29069-29071	NN	denotes	KO
T15698	29072-29074	NN	denotes	SP
T15699	29075-29080	NN	denotes	cells
T15700	29080-29081	-COMMA-	denotes	,
T15701	29082-29089	IN	denotes	whereas
T15702	29090-29095	DT	denotes	those
T15703	29096-29101	VB	denotes	shown
T15704	29102-29104	IN	denotes	in
T15705	29105-29110	NNP	denotes	Figs.
T15706	29111-29112	CD	denotes	3
T15707	29113-29116	CC	denotes	and
T15708	29117-29118	CD	denotes	4
T15709	29119-29122	VB	denotes	are
T15710	29123-29127	IN	denotes	from
T15711	29128-29133	JJ	denotes	total
T15712	29134-29139	NN	denotes	Runx3
T15713	29140-29142	NN	denotes	KO
T15714	29143-29146	NN	denotes	CD8
T15715	29147-29152	NN	denotes	cells
T16229	29155-29165	JJ	denotes	FACS-based
T16230	29166-29178	NN	denotes	cytotoxicity
T16231	29179-29184	NN	denotes	assay
T16232	29186-29188	TO	denotes	To
T16233	29189-29196	VB	denotes	measure
T16234	29197-29209	NN	denotes	cytotoxicity
T16235	29209-29210	-COMMA-	denotes	,
T16236	29211-29214	NN	denotes	EL4
T16237	29215-29222	NN	denotes	thymoma
T16238	29223-29229	NN	denotes	target
T16239	29230-29235	NN	denotes	cells
T16240	29236-29240	VB	denotes	were
T16241	29241-29247	VB	denotes	loaded
T16242	29248-29252	IN	denotes	with
T16243	29253-29254	CD	denotes	0
T16244	29255-29257	CC	denotes	or
T16245	29258-29259	CD	denotes	1
T16246	29260-29262	NN	denotes	μM
T16247	29263-29267	NN	denotes	Gp33
T16248	29268-29275	NN	denotes	peptide
T16249	29276-29279	IN	denotes	for
T16250	29280-29281	CD	denotes	2
T16251	29282-29283	NN	denotes	h
T16252	29284-29290	IN	denotes	before
T16253	29291-29292	DT	denotes	a
T16254	29293-29296	JJ	denotes	2-h
T16255	29297-29309	NN	denotes	coincubation
T16256	29310-29314	IN	denotes	with
T16257	29315-29318	NN	denotes	P14
T16258	29319-29323	JJ	denotes	CD8+
T16259	29324-29325	NN	denotes	T
T16260	29326-29331	NN	denotes	cells
T16261	29332-29334	IN	denotes	at
T16262	29335-29338	DT	denotes	the
T16263	29339-29357	JJ	denotes	effector-to-target
T16264	29358-29364	NN	denotes	ratios
T16265	29365-29374	VB	denotes	indicated
T16266	29375-29377	IN	denotes	in
T16267	29378-29381	DT	denotes	the
T16268	29382-29389	NN	denotes	figures
T16269	29390-29392	IN	denotes	in
T16270	29393-29400	JJ	denotes	96-well
T16271	29401-29413	JJ	denotes	round-bottom
T16272	29414-29420	NN	denotes	plates
T16273	29422-29427	IN	denotes	After
T16274	29428-29431	DT	denotes	the
T16275	29432-29444	NN	denotes	coincubation
T16276	29445-29451	NN	denotes	period
T16277	29451-29452	-COMMA-	denotes	,
T16278	29453-29458	NN	denotes	cells
T16279	29459-29463	VB	denotes	were
T16280	29464-29471	VB	denotes	stained
T16281	29472-29476	IN	denotes	with
T16282	29477-29484	NN	denotes	Annexin
T16283	29485-29491	NN	denotes	V–FITC
T16284	29492-29495	CC	denotes	and
T16285	29496-29520	NN	denotes	anti-CD8–allophycocyanin
T16286	29522-29526	NN	denotes	Data
T16287	29527-29535	NN	denotes	analysis
T16288	29536-29539	VB	denotes	was
T16289	29540-29549	VB	denotes	performed
T16290	29550-29554	IN	denotes	with
T16291	29555-29561	NN	denotes	FlowJo
T16292	29562-29570	NN	denotes	software
T16293	29571-29572	-LRB-	denotes	(
T16294	29572-29576	NNP	denotes	Tree
T16295	29577-29581	NNP	denotes	Star
T16296	29581-29582	-COMMA-	denotes	,
T16297	29583-29587	NNP	denotes	Inc.
T16298	29587-29588	-RRB-	denotes	)
T16299	29588-29589	-COLON-	denotes	;
T16300	29590-29593	NN	denotes	EL4
T16301	29594-29600	NN	denotes	target
T16302	29601-29606	NN	denotes	cells
T16303	29607-29608	-LRB-	denotes	(
T16304	29608-29620	JJ	denotes	CD8-negative
T16305	29621-29627	NN	denotes	events
T16306	29627-29628	-RRB-	denotes	)
T16307	29629-29633	VB	denotes	were
T16308	29634-29639	VB	denotes	gated
T16309	29639-29640	-COMMA-	denotes	,
T16310	29641-29644	CC	denotes	and
T16311	29645-29648	DT	denotes	the
T16312	29649-29659	NN	denotes	percentage
T16313	29660-29662	IN	denotes	of
T16314	29663-29670	NN	denotes	Annexin
T16315	29671-29673	JJ	denotes	V+
T16316	29674-29680	NN	denotes	target
T16317	29681-29686	NN	denotes	cells
T16318	29687-29690	VB	denotes	was
T16319	29691-29701	VB	denotes	determined
T16510	29704-29712	NN	denotes	Cytokine
T16511	29713-29716	CC	denotes	and
T16512	29717-29724	NN	denotes	surface
T16513	29725-29731	NN	denotes	marker
T16514	29732-29740	NN	denotes	staining
T16515	29742-29744	TO	denotes	To
T16516	29745-29751	VB	denotes	assess
T16517	29752-29760	NN	denotes	cytokine
T16518	29761-29771	NN	denotes	production
T16519	29771-29772	-COMMA-	denotes	,
T16520	29773-29778	NN	denotes	cells
T16521	29779-29783	VB	denotes	were
T16522	29784-29796	VB	denotes	restimulated
T16523	29797-29801	IN	denotes	with
T16524	29802-29804	CD	denotes	10
T16525	29805-29807	NN	denotes	nM
T16526	29808-29811	NN	denotes	PMA
T16527	29812-29813	SYM	denotes	+
T16528	29814-29815	CD	denotes	1
T16529	29816-29818	NN	denotes	μM
T16530	29819-29828	NN	denotes	ionomycin
T16531	29829-29832	IN	denotes	for
T16532	29833-29834	CD	denotes	6
T16533	29835-29836	NN	denotes	h
T16534	29837-29838	-LRB-	denotes	(
T16535	29838-29844	IN	denotes	unless
T16536	29845-29854	VB	denotes	indicated
T16537	29855-29864	RB	denotes	otherwise
T16538	29865-29867	IN	denotes	in
T16539	29868-29871	DT	denotes	the
T16540	29872-29879	NN	denotes	figures
T16541	29879-29880	-RRB-	denotes	)
T16542	29880-29881	-COMMA-	denotes	,
T16543	29882-29885	CC	denotes	and
T16544	29886-29899	JJ	denotes	intracellular
T16545	29900-29908	NN	denotes	cytokine
T16546	29909-29915	NN	denotes	stains
T16547	29916-29920	VB	denotes	were
T16548	29921-29930	VB	denotes	performed
T16549	29931-29933	IN	denotes	as
T16550	29934-29944	RB	denotes	previously
T16551	29945-29954	VB	denotes	described
T16552	29955-29956	-LRB-	denotes	(
T16553	29956-29958	CD	denotes	28
T16554	29958-29959	-RRB-	denotes	)
T16555	29961-29963	TO	denotes	To
T16556	29964-29970	VB	denotes	detect
T16557	29971-29981	NN	denotes	expression
T16558	29982-29984	IN	denotes	of
T16559	29985-29992	NN	denotes	surface
T16560	29993-30002	NN	denotes	molecules
T16561	30002-30003	-COMMA-	denotes	,
T16562	30004-30009	NN	denotes	cells
T16563	30010-30014	VB	denotes	were
T16564	30015-30021	VB	denotes	washed
T16565	30022-30024	IN	denotes	in
T16566	30025-30028	NNP	denotes	PBS
T16567	30028-30029	-COMMA-	denotes	,
T16568	30030-30041	VB	denotes	resuspended
T16569	30042-30044	IN	denotes	in
T16570	30045-30049	NN	denotes	FACS
T16571	30050-30054	NN	denotes	wash
T16572	30055-30061	NN	denotes	buffer
T16573	30062-30063	-LRB-	denotes	(
T16574	30063-30064	CD	denotes	3
T16575	30064-30065	NN	denotes	%
T16576	30066-30069	NN	denotes	FBS
T16577	30069-30070	-COMMA-	denotes	,
T16578	30071-30074	CD	denotes	0.1
T16579	30074-30075	NN	denotes	%
T16580	30076-30082	NN	denotes	sodium
T16581	30083-30088	NN	denotes	azide
T16582	30088-30089	-COMMA-	denotes	,
T16583	30090-30092	CD	denotes	30
T16584	30093-30095	NN	denotes	mM
T16585	30096-30101	NNP	denotes	Hepes
T16586	30101-30102	-COMMA-	denotes	,
T16587	30103-30105	CD	denotes	1×
T16588	30106-30109	NNP	denotes	PBS
T16589	30109-30110	-RRB-	denotes	)
T16590	30111-30121	VB	denotes	containing
T16591	30122-30125	DT	denotes	the
T16592	30126-30136	NN	denotes	antibodies
T16593	30137-30146	VB	denotes	indicated
T16594	30147-30149	IN	denotes	in
T16595	30150-30153	DT	denotes	the
T16596	30154-30161	NN	denotes	figures
T16597	30162-30164	IN	denotes	at
T16598	30165-30175	RB	denotes	previously
T16599	30176-30185	VB	denotes	optimized
T16600	30186-30200	NN	denotes	concentrations
T16601	30200-30201	-COMMA-	denotes	,
T16602	30202-30211	VB	denotes	incubated
T16603	30212-30215	IN	denotes	for
T16604	30216-30218	CD	denotes	15
T16605	30219-30222	NN	denotes	min
T16606	30223-30225	IN	denotes	at
T16607	30226-30230	NN	denotes	room
T16608	30231-30242	NN	denotes	temperature
T16609	30243-30244	-LRB-	denotes	(
T16610	30244-30246	NN	denotes	RT
T16611	30246-30247	-RRB-	denotes	)
T16612	30247-30248	-COMMA-	denotes	,
T16613	30249-30255	VB	denotes	washed
T16614	30255-30256	-COMMA-	denotes	,
T16615	30257-30260	CC	denotes	and
T16616	30261-30272	VB	denotes	resuspended
T16617	30273-30275	IN	denotes	in
T16618	30276-30277	CD	denotes	2
T16619	30277-30278	NN	denotes	%
T16620	30279-30291	NN	denotes	formaldehyde
T16621	30292-30300	JJ	denotes	fixative
T16622	30301-30309	NN	denotes	solution
T16623	30310-30316	IN	denotes	before
T16624	30317-30328	NN	denotes	acquisition
T16625	30329-30331	IN	denotes	on
T16626	30332-30333	DT	denotes	a
T16627	30334-30345	NN	denotes	FACSCalibur
T16628	30346-30347	-LRB-	denotes	(
T16629	30347-30349	NN	denotes	BD
T16630	30349-30350	-RRB-	denotes	)
T16785	30353-30363	JJ	denotes	Retroviral
T16786	30364-30376	NN	denotes	transduction
T16787	30377-30379	IN	denotes	of
T16788	30380-30387	JJ	denotes	primary
T16789	30388-30392	JJ	denotes	CD8+
T16790	30393-30394	NN	denotes	T
T16791	30395-30400	NN	denotes	cells
T16792	30402-30405	IN	denotes	For
T16793	30406-30418	NN	denotes	transduction
T16794	30419-30430	NN	denotes	experiments
T16795	30430-30431	-COMMA-	denotes	,
T16796	30432-30437	JJ	denotes	viral
T16797	30438-30450	NN	denotes	supernatants
T16798	30451-30455	VB	denotes	were
T16799	30456-30465	VB	denotes	generated
T16800	30466-30468	IN	denotes	by
T16801	30469-30476	NN	denotes	calcium
T16802	30477-30486	NN	denotes	phosphate
T16803	30487-30499	NN	denotes	transfection
T16804	30500-30502	IN	denotes	of
T16805	30503-30510	NN	denotes	Phoenix
T16806	30511-30516	NN	denotes	cells
T16807	30517-30520	CC	denotes	and
T16808	30521-30534	NN	denotes	concentration
T16809	30535-30537	IN	denotes	by
T16810	30538-30547	JJ	denotes	overnight
T16811	30548-30562	NN	denotes	centrifugation
T16812	30563-30565	IN	denotes	at
T16813	30566-30571	CD	denotes	6,000
T16814	30572-30573	NN	denotes	g
T16815	30575-30577	IN	denotes	At
T16816	30578-30581	CD	denotes	∼42
T16817	30582-30583	NN	denotes	h
T16818	30584-30589	IN	denotes	after
T16819	30590-30593	DT	denotes	the
T16820	30594-30601	JJ	denotes	initial
T16821	30602-30605	NN	denotes	TCR
T16822	30606-30616	NN	denotes	activation
T16823	30617-30619	IN	denotes	of
T16824	30620-30623	CD	denotes	106
T16825	30624-30628	JJ	denotes	CD8+
T16826	30629-30630	NN	denotes	T
T16827	30631-30636	NN	denotes	cells
T16828	30637-30640	IN	denotes	per
T16829	30641-30645	RB	denotes	well
T16830	30646-30648	IN	denotes	in
T16831	30649-30656	JJ	denotes	12-well
T16832	30657-30663	NN	denotes	plates
T16833	30663-30664	-COMMA-	denotes	,
T16834	30665-30668	DT	denotes	the
T16835	30669-30676	NN	denotes	culture
T16836	30677-30682	NN	denotes	media
T16837	30683-30686	VB	denotes	was
T16838	30687-30694	VB	denotes	removed
T16839	30695-30698	CC	denotes	and
T16840	30699-30707	VB	denotes	replaced
T16841	30708-30712	IN	denotes	with
T16842	30713-30721	JJ	denotes	complete
T16843	30722-30727	NN	denotes	media
T16844	30728-30740	VB	denotes	supplemented
T16845	30741-30745	IN	denotes	with
T16846	30746-30747	CD	denotes	8
T16847	30748-30753	NN	denotes	μg/ml
T16848	30754-30763	NN	denotes	polybrene
T16849	30764-30774	VB	denotes	containing
T16850	30775-30780	JJ	denotes	fresh
T16851	30781-30785	CC	denotes	plus
T16852	30786-30798	JJ	denotes	concentrated
T16853	30799-30804	NN	denotes	virus
T16854	30806-30809	DT	denotes	The
T16855	30810-30816	NN	denotes	plates
T16856	30817-30821	VB	denotes	were
T16857	30822-30833	VB	denotes	centrifuged
T16858	30834-30836	IN	denotes	at
T16859	30837-30840	CD	denotes	700
T16860	30841-30842	NN	denotes	g
T16861	30843-30846	IN	denotes	for
T16862	30847-30848	CD	denotes	1
T16863	30849-30850	NN	denotes	h
T16864	30851-30853	IN	denotes	at
T16865	30854-30856	NN	denotes	RT
T16866	30857-30863	IN	denotes	before
T16867	30864-30873	VB	denotes	returning
T16868	30874-30876	TO	denotes	to
T16869	30877-30881	NN	denotes	37°C
T16870	30882-30885	IN	denotes	for
T16871	30886-30888	DT	denotes	an
T16872	30889-30899	JJ	denotes	additional
T16873	30900-30901	CD	denotes	5
T16874	30902-30903	NN	denotes	h
T16875	30905-30915	JJ	denotes	Retroviral
T16876	30916-30926	NN	denotes	constructs
T16877	30927-30930	IN	denotes	for
T16878	30931-30941	NN	denotes	Eomes-VP16
T16879	30942-30945	CC	denotes	and
T16880	30946-30949	DT	denotes	the
T16881	30950-30953	NN	denotes	MIG
T16882	30954-30961	NN	denotes	control
T16883	30962-30967	JJ	denotes	empty
T16884	30968-30974	NN	denotes	vector
T16885	30975-30979	VB	denotes	were
T16886	30980-30981	DT	denotes	a
T16887	30982-30986	NN	denotes	gift
T16888	30987-30991	IN	denotes	from
T16889	30992-30996	NNP	denotes	S.L.
T16890	30997-31003	NNP	denotes	Reiner
T16891	31004-31005	-LRB-	denotes	(
T16892	31005-31015	NNP	denotes	University
T16893	31016-31018	IN	denotes	of
T16894	31019-31031	NNP	denotes	Pennsylvania
T16895	31031-31032	-COMMA-	denotes	,
T16896	31033-31045	NNP	denotes	Philadelphia
T16897	31045-31046	-COMMA-	denotes	,
T16898	31047-31049	NNP	denotes	PA
T16899	31049-31050	-RRB-	denotes	)
T16900	31051-31052	-LRB-	denotes	(
T16901	31052-31053	CD	denotes	8
T16902	31053-31054	-RRB-	denotes	)
T16963	31057-31061	NN	denotes	ChIP
T16964	31062-31065	CC	denotes	and
T16965	31066-31075	JJ	denotes	real-time
T16966	31076-31079	NN	denotes	PCR
T16967	31080-31088	NN	denotes	analysis
T16968	31090-31092	CD	denotes	20
T16969	31093-31094	CC	denotes	×
T16970	31095-31098	CD	denotes	106
T16971	31099-31103	JJ	denotes	CD8+
T16972	31104-31105	NN	denotes	T
T16973	31106-31111	NN	denotes	cells
T16974	31112-31115	IN	denotes	per
T16975	31116-31135	NN	denotes	immunoprecipitation
T16976	31136-31140	VB	denotes	were
T16977	31141-31146	VB	denotes	fixed
T16978	31147-31149	IN	denotes	by
T16979	31150-31156	VB	denotes	adding
T16980	31157-31158	DT	denotes	a
T16981	31159-31165	JJ	denotes	1/10th
T16982	31166-31172	NN	denotes	volume
T16983	31173-31175	IN	denotes	of
T16984	31176-31184	NN	denotes	fixation
T16985	31185-31193	NN	denotes	solution
T16986	31194-31195	-LRB-	denotes	(
T16987	31195-31199	CD	denotes	11.1
T16988	31199-31200	NN	denotes	%
T16989	31201-31213	NN	denotes	formaldehyde
T16990	31213-31214	-COMMA-	denotes	,
T16991	31215-31218	CD	denotes	100
T16992	31219-31221	NN	denotes	mM
T16993	31222-31226	NN	denotes	NaCl
T16994	31226-31227	-COMMA-	denotes	,
T16995	31228-31229	CD	denotes	1
T16996	31230-31232	NN	denotes	mM
T16997	31233-31237	NN	denotes	EDTA
T16998	31237-31238	-COMMA-	denotes	,
T16999	31239-31242	CD	denotes	0.5
T17000	31243-31245	NN	denotes	mM
T17001	31246-31250	NN	denotes	EGTA
T17002	31250-31251	-COMMA-	denotes	,
T17003	31252-31254	CD	denotes	50
T17004	31255-31257	NN	denotes	mM
T17005	31258-31263	NN	denotes	Hepes
T17006	31263-31264	-RRB-	denotes	)
T17007	31265-31267	TO	denotes	to
T17008	31268-31269	CD	denotes	1
T17009	31270-31276	NN	denotes	volume
T17010	31277-31279	IN	denotes	of
T17011	31280-31287	NN	denotes	culture
T17012	31288-31293	NN	denotes	media
T17013	31294-31297	CC	denotes	and
T17014	31298-31302	VB	denotes	were
T17015	31303-31312	VB	denotes	incubated
T17016	31313-31316	IN	denotes	for
T17017	31317-31319	CD	denotes	10
T17018	31320-31322	CC	denotes	or
T17019	31323-31325	CD	denotes	30
T17020	31326-31329	NN	denotes	min
T17021	31330-31332	IN	denotes	at
T17022	31333-31335	NN	denotes	RT
T17023	31337-31345	NN	denotes	Fixation
T17024	31346-31349	VB	denotes	was
T17025	31350-31357	VB	denotes	stopped
T17026	31358-31362	IN	denotes	with
T17027	31363-31366	CD	denotes	120
T17028	31367-31369	NN	denotes	mM
T17029	31370-31377	NN	denotes	glycine
T17030	31378-31380	IN	denotes	on
T17031	31381-31384	NN	denotes	ice
T17032	31385-31388	IN	denotes	for
T17033	31389-31390	CD	denotes	5
T17034	31391-31394	NN	denotes	min
T17035	31396-31401	VB	denotes	Fixed
T17036	31402-31407	NN	denotes	cells
T17037	31408-31412	VB	denotes	were
T17038	31413-31419	VB	denotes	washed
T17039	31420-31422	CD	denotes	2×
T17040	31423-31427	IN	denotes	with
T17041	31428-31432	JJ	denotes	cold
T17042	31433-31436	NNP	denotes	PBS
T17043	31436-31437	-COMMA-	denotes	,
T17044	31438-31440	CD	denotes	1×
T17045	31441-31445	IN	denotes	with
T17046	31446-31450	JJ	denotes	cold
T17047	31451-31459	NN	denotes	solution
T17048	31460-31461	CD	denotes	I
T17049	31462-31463	-LRB-	denotes	(
T17050	31463-31465	CD	denotes	10
T17051	31466-31468	NN	denotes	mM
T17052	31469-31473	NN	denotes	Tris
T17053	31474-31475	-LRB-	denotes	[
T17054	31475-31477	NN	denotes	pH
T17055	31478-31481	CD	denotes	7.5
T17056	31481-31482	-RRB-	denotes	]
T17057	31482-31483	-COMMA-	denotes	,
T17058	31484-31486	CD	denotes	10
T17059	31487-31489	NN	denotes	mM
T17060	31490-31494	NN	denotes	EDTA
T17061	31494-31495	-COMMA-	denotes	,
T17062	31496-31499	CD	denotes	0.5
T17063	31500-31502	NN	denotes	mM
T17064	31503-31507	NN	denotes	EGTA
T17065	31507-31508	-COMMA-	denotes	,
T17066	31509-31510	CD	denotes	1
T17067	31510-31511	NN	denotes	%
T17068	31512-31518	NN	denotes	Triton
T17069	31519-31524	NN	denotes	X-100
T17070	31524-31525	-RRB-	denotes	)
T17071	31525-31526	-COMMA-	denotes	,
T17072	31527-31530	CC	denotes	and
T17073	31531-31533	CD	denotes	1×
T17074	31534-31538	IN	denotes	with
T17075	31539-31543	JJ	denotes	cold
T17076	31544-31552	NN	denotes	solution
T17077	31553-31555	CD	denotes	II
T17078	31556-31557	-LRB-	denotes	(
T17079	31557-31559	CD	denotes	10
T17080	31560-31562	NN	denotes	mM
T17081	31563-31567	NN	denotes	Tris
T17082	31568-31569	-LRB-	denotes	[
T17083	31569-31571	NN	denotes	pH
T17084	31572-31575	CD	denotes	7.5
T17085	31575-31576	-RRB-	denotes	]
T17086	31576-31577	-COMMA-	denotes	,
T17087	31578-31579	CD	denotes	1
T17088	31580-31582	NN	denotes	mM
T17089	31583-31587	NN	denotes	EDTA
T17090	31587-31588	-COMMA-	denotes	,
T17091	31589-31592	CD	denotes	0.5
T17092	31593-31595	NN	denotes	mM
T17093	31596-31600	NN	denotes	EGTA
T17094	31600-31601	-COMMA-	denotes	,
T17095	31602-31605	CD	denotes	200
T17096	31606-31608	NN	denotes	mM
T17097	31609-31613	NN	denotes	NaCl
T17098	31613-31614	-RRB-	denotes	)
T17099	31616-31621	IN	denotes	After
T17100	31622-31628	NN	denotes	washes
T17101	31628-31629	-COMMA-	denotes	,
T17102	31630-31634	NN	denotes	cell
T17103	31635-31642	NN	denotes	pellets
T17104	31643-31647	VB	denotes	were
T17105	31648-31659	VB	denotes	resuspended
T17106	31660-31662	IN	denotes	at
T17107	31663-31665	CD	denotes	40
T17108	31666-31667	CC	denotes	×
T17109	31668-31671	CD	denotes	106
T17110	31672-31680	NN	denotes	cells/ml
T17111	31681-31683	IN	denotes	in
T17112	31684-31688	NN	denotes	ChIP
T17113	31689-31694	NN	denotes	lysis
T17114	31695-31701	NN	denotes	buffer
T17115	31702-31703	-LRB-	denotes	(
T17116	31703-31706	CD	denotes	150
T17117	31707-31709	NN	denotes	mM
T17118	31710-31714	NN	denotes	NaCl
T17119	31714-31715	-COMMA-	denotes	,
T17120	31716-31718	CD	denotes	25
T17121	31719-31721	NN	denotes	mM
T17122	31722-31726	NN	denotes	Tris
T17123	31727-31728	-LRB-	denotes	[
T17124	31728-31730	NN	denotes	pH
T17125	31731-31734	CD	denotes	7.5
T17126	31734-31735	-RRB-	denotes	]
T17127	31735-31736	-COMMA-	denotes	,
T17128	31737-31738	CD	denotes	1
T17129	31738-31739	NN	denotes	%
T17130	31740-31746	NN	denotes	Triton
T17131	31747-31752	NN	denotes	X-100
T17132	31752-31753	-COMMA-	denotes	,
T17133	31754-31757	CD	denotes	0.1
T17134	31757-31758	NN	denotes	%
T17135	31759-31762	NN	denotes	SDS
T17136	31762-31763	-COMMA-	denotes	,
T17137	31764-31767	CD	denotes	0.5
T17138	31767-31768	NN	denotes	%
T17139	31769-31781	NN	denotes	deoxycholate
T17140	31782-31786	CC	denotes	plus
T17141	31787-31795	NN	denotes	protease
T17142	31796-31799	CC	denotes	and
T17143	31800-31811	NN	denotes	phosphatase
T17144	31812-31822	NN	denotes	inhibitors
T17145	31822-31823	-RRB-	denotes	)
T17146	31823-31824	-COMMA-	denotes	,
T17147	31825-31828	CC	denotes	and
T17148	31829-31838	NN	denotes	chromatin
T17149	31839-31842	VB	denotes	was
T17150	31843-31850	VB	denotes	sheared
T17151	31851-31855	IN	denotes	with
T17152	31856-31857	DT	denotes	a
T17153	31858-31867	NN	denotes	sonicator
T17154	31868-31870	TO	denotes	to
T17155	31871-31876	VB	denotes	yield
T17156	31877-31885	JJ	denotes	0.5–1-kb
T17157	31886-31889	NN	denotes	DNA
T17158	31890-31899	NN	denotes	fragments
T17159	31901-31906	IN	denotes	After
T17160	31907-31918	VB	denotes	preclearing
T17161	31919-31922	DT	denotes	the
T17162	31923-31930	JJ	denotes	sheared
T17163	31931-31940	NN	denotes	chromatin
T17164	31941-31945	IN	denotes	with
T17165	31946-31953	NN	denotes	protein
T17166	31954-31965	NN	denotes	A–sepharose
T17167	31966-31971	NN	denotes	beads
T17168	31972-31975	CC	denotes	and
T17169	31976-31984	VB	denotes	removing
T17170	31985-31986	CD	denotes	5
T17171	31986-31987	NN	denotes	%
T17172	31988-31990	IN	denotes	as
T17173	31991-31996	NN	denotes	input
T17174	31997-32006	NN	denotes	chromatin
T17175	32006-32007	-COMMA-	denotes	,
T17176	32008-32027	NN	denotes	immunoprecipitation
T17177	32028-32031	VB	denotes	was
T17178	32032-32041	VB	denotes	performed
T17179	32042-32044	IN	denotes	by
T17180	32045-32051	VB	denotes	adding
T17181	32052-32061	VB	denotes	optimized
T17182	32062-32070	NN	denotes	antibody
T17183	32071-32078	NN	denotes	amounts
T17184	32079-32080	-LRB-	denotes	(
T17185	32080-32083	IN	denotes	per
T17186	32084-32086	CD	denotes	20
T17187	32087-32088	-COLON-	denotes	×
T17188	32089-32092	CD	denotes	106
T17189	32093-32097	NN	denotes	cell
T17190	32098-32109	NN	denotes	equivalents
T17191	32109-32110	-COLON-	denotes	:
T17192	32111-32114	CD	denotes	2.5
T17193	32115-32117	NN	denotes	μg
T17194	32118-32128	NN	denotes	anti-Eomes
T17195	32128-32129	-COMMA-	denotes	,
T17196	32130-32131	CD	denotes	1
T17197	32131-32132	SYM	denotes	:
T17198	32132-32135	CD	denotes	100
T17199	32136-32144	NN	denotes	dilution
T17200	32145-32155	NN	denotes	anti-Runx3
T17201	32155-32156	-RRB-	denotes	)
T17202	32156-32157	-COMMA-	denotes	,
T17203	32158-32166	VB	denotes	followed
T17204	32167-32169	IN	denotes	by
T17205	32170-32179	JJ	denotes	overnight
T17206	32180-32190	NN	denotes	incubation
T17207	32191-32193	IN	denotes	at
T17208	32194-32197	NN	denotes	4°C
T17209	32197-32198	-COLON-	denotes	;
T17210	32199-32206	NN	denotes	protein
T17211	32207-32218	NN	denotes	A–sepharose
T17212	32219-32224	NN	denotes	beads
T17213	32225-32229	VB	denotes	were
T17214	32230-32235	VB	denotes	added
T17215	32236-32239	IN	denotes	for
T17216	32240-32243	DT	denotes	the
T17217	32244-32248	JJ	denotes	last
T17218	32249-32250	CD	denotes	3
T17219	32251-32252	NN	denotes	h
T17220	32253-32255	IN	denotes	of
T17221	32256-32259	DT	denotes	the
T17222	32260-32270	NN	denotes	incubation
T17223	32271-32277	NN	denotes	period
T17224	32279-32284	NN	denotes	Beads
T17225	32285-32289	VB	denotes	were
T17226	32290-32296	VB	denotes	washed
T17227	32297-32299	CD	denotes	2×
T17228	32300-32304	IN	denotes	with
T17229	32305-32309	NN	denotes	RIPA
T17230	32310-32316	NN	denotes	buffer
T17231	32317-32318	-LRB-	denotes	(
T17232	32318-32320	CD	denotes	50
T17233	32321-32323	NN	denotes	mM
T17234	32324-32328	NN	denotes	Tris
T17235	32329-32330	-LRB-	denotes	[
T17236	32330-32332	NN	denotes	pH
T17237	32333-32334	CD	denotes	8
T17238	32334-32335	-RRB-	denotes	]
T17239	32335-32336	-COMMA-	denotes	,
T17240	32337-32340	CD	denotes	150
T17241	32341-32343	NN	denotes	mM
T17242	32344-32348	NN	denotes	NaCl
T17243	32348-32349	-COMMA-	denotes	,
T17244	32350-32351	CD	denotes	1
T17245	32352-32354	NN	denotes	mM
T17246	32355-32359	NN	denotes	EDTA
T17247	32359-32360	-COMMA-	denotes	,
T17248	32361-32362	CD	denotes	1
T17249	32362-32363	NN	denotes	%
T17250	32364-32369	NN	denotes	NP-40
T17251	32369-32370	-COMMA-	denotes	,
T17252	32371-32374	CD	denotes	0.1
T17253	32374-32375	NN	denotes	%
T17254	32376-32379	NN	denotes	SDS
T17255	32379-32380	-COMMA-	denotes	,
T17256	32381-32384	CD	denotes	0.5
T17257	32384-32385	NN	denotes	%
T17258	32386-32398	NN	denotes	deoxycholate
T17259	32398-32399	-RRB-	denotes	)
T17260	32399-32400	-COMMA-	denotes	,
T17261	32401-32403	CD	denotes	1×
T17262	32404-32408	IN	denotes	with
T17263	32409-32413	JJ	denotes	high
T17264	32414-32418	NN	denotes	salt
T17265	32419-32425	NN	denotes	buffer
T17266	32426-32427	-LRB-	denotes	(
T17267	32427-32429	CD	denotes	50
T17268	32430-32432	NN	denotes	mM
T17269	32433-32437	NN	denotes	Tris
T17270	32438-32439	-LRB-	denotes	[
T17271	32439-32441	NN	denotes	pH
T17272	32442-32443	CD	denotes	8
T17273	32443-32444	-RRB-	denotes	]
T17274	32444-32445	-COMMA-	denotes	,
T17275	32446-32449	CD	denotes	500
T17276	32450-32452	NN	denotes	mM
T17277	32453-32457	NN	denotes	NaCl
T17278	32457-32458	-COMMA-	denotes	,
T17279	32459-32460	CD	denotes	1
T17280	32461-32463	NN	denotes	mM
T17281	32464-32468	NN	denotes	EDTA
T17282	32468-32469	-COMMA-	denotes	,
T17283	32470-32471	CD	denotes	1
T17284	32471-32472	NN	denotes	%
T17285	32473-32478	NN	denotes	NP-40
T17286	32478-32479	-COMMA-	denotes	,
T17287	32480-32483	CD	denotes	0.1
T17288	32483-32484	NN	denotes	%
T17289	32485-32488	NN	denotes	SDS
T17290	32488-32489	-RRB-	denotes	)
T17291	32489-32490	-COMMA-	denotes	,
T17292	32491-32494	CC	denotes	and
T17293	32495-32497	NN	denotes	1×
T17294	32498-32502	IN	denotes	with
T17295	32503-32505	NN	denotes	TE
T17296	32506-32512	NN	denotes	buffer
T17297	32514-32519	IN	denotes	After
T17298	32520-32523	DT	denotes	the
T17299	32524-32528	JJ	denotes	last
T17300	32529-32533	NN	denotes	wash
T17301	32533-32534	-COMMA-	denotes	,
T17302	32535-32538	NN	denotes	DNA
T17303	32539-32542	VB	denotes	was
T17304	32543-32549	VB	denotes	eluted
T17305	32550-32552	IN	denotes	by
T17306	32553-32565	VB	denotes	resuspending
T17307	32566-32569	DT	denotes	the
T17308	32570-32575	NN	denotes	beads
T17309	32576-32578	IN	denotes	in
T17310	32579-32586	NN	denotes	elution
T17311	32587-32593	NN	denotes	buffer
T17312	32594-32595	-LRB-	denotes	(
T17313	32595-32596	CD	denotes	1
T17314	32596-32597	NN	denotes	%
T17315	32598-32601	NN	denotes	SDS
T17316	32601-32602	-COMMA-	denotes	,
T17317	32603-32606	CD	denotes	100
T17318	32607-32609	NN	denotes	mM
T17319	32610-32616	NN	denotes	NaHCO3
T17320	32616-32617	-RRB-	denotes	)
T17321	32619-32623	CC	denotes	Both
T17322	32624-32629	NN	denotes	input
T17323	32630-32633	CC	denotes	and
T17324	32634-32638	NN	denotes	ChIP
T17325	32639-32648	NN	denotes	chromatin
T17326	32649-32653	VB	denotes	were
T17327	32654-32658	RB	denotes	then
T17328	32659-32666	VB	denotes	treated
T17329	32667-32671	IN	denotes	with
T17330	32672-32677	NN	denotes	RNase
T17331	32678-32679	NN	denotes	A
T17332	32680-32681	-LRB-	denotes	(
T17333	32681-32682	CD	denotes	5
T17334	32683-32685	NN	denotes	μg
T17335	32686-32691	NN	denotes	total
T17336	32691-32692	-RRB-	denotes	)
T17337	32693-32696	IN	denotes	for
T17338	32697-32698	CD	denotes	1
T17339	32699-32700	NN	denotes	h
T17340	32701-32703	IN	denotes	at
T17341	32704-32708	NN	denotes	37°C
T17342	32708-32709	-COMMA-	denotes	,
T17343	32710-32718	VB	denotes	followed
T17344	32719-32721	IN	denotes	by
T17345	32722-32725	DT	denotes	the
T17346	32726-32734	NN	denotes	addition
T17347	32735-32737	IN	denotes	of
T17348	32738-32748	NN	denotes	proteinase
T17349	32749-32750	NN	denotes	K
T17350	32751-32752	-LRB-	denotes	(
T17351	32752-32755	CD	denotes	100
T17352	32756-32758	NN	denotes	μg
T17353	32759-32764	NN	denotes	total
T17354	32764-32765	-RRB-	denotes	)
T17355	32766-32769	CC	denotes	and
T17356	32770-32779	JJ	denotes	overnight
T17357	32780-32790	NN	denotes	incubation
T17358	32791-32793	IN	denotes	at
T17359	32794-32798	NN	denotes	65°C
T17360	32799-32801	TO	denotes	to
T17361	32802-32809	VB	denotes	reverse
T17362	32810-32823	NN	denotes	cross-linking
T17363	32825-32828	NN	denotes	DNA
T17364	32829-32832	VB	denotes	was
T17365	32833-32837	RB	denotes	then
T17366	32838-32846	VB	denotes	purified
T17367	32847-32851	IN	denotes	with
T17368	32852-32860	JJ	denotes	QIAquick
T17369	32861-32868	NN	denotes	columns
T17370	32869-32870	-LRB-	denotes	(
T17371	32870-32873	NN	denotes	Gel
T17372	32874-32884	NN	denotes	Extraction
T17373	32885-32888	NN	denotes	Kit
T17374	32888-32889	-COLON-	denotes	;
T17375	32890-32896	NN	denotes	QIAGEN
T17376	32896-32897	-RRB-	denotes	)
T17377	32898-32907	VB	denotes	according
T17378	32908-32910	TO	denotes	to
T17379	32911-32914	DT	denotes	the
T17380	32915-32927	NN	denotes	manufacturer
T17381	32927-32929	POS	denotes	's
T17382	32930-32942	NN	denotes	instructions
T17383	32943-32946	CC	denotes	and
T17384	32947-32958	VB	denotes	resuspended
T17385	32959-32961	IN	denotes	in
T17386	32962-32963	DT	denotes	a
T17387	32964-32969	JJ	denotes	50-μl
T17388	32970-32976	NN	denotes	volume
T17389	32978-32981	IN	denotes	For
T17390	32982-32991	JJ	denotes	real-time
T17391	32992-32995	NN	denotes	PCR
T17392	32996-33005	NN	denotes	detection
T17393	33006-33008	IN	denotes	of
T17394	33009-33027	JJ	denotes	immunoprecipitated
T17395	33028-33035	NN	denotes	targets
T17396	33036-33041	VB	denotes	using
T17397	33042-33045	DT	denotes	the
T17398	33046-33050	NNP	denotes	SYBR
T17399	33051-33056	NNP	denotes	Green
T17400	33057-33060	NN	denotes	PCR
T17401	33061-33064	NN	denotes	Kit
T17402	33064-33065	-COMMA-	denotes	,
T17403	33066-33067	DT	denotes	a
T17404	33068-33076	JJ	denotes	standard
T17405	33077-33082	NN	denotes	curve
T17406	33083-33086	VB	denotes	was
T17407	33087-33095	VB	denotes	obtained
T17408	33096-33100	IN	denotes	with
T17409	33101-33107	JJ	denotes	serial
T17410	33108-33117	NN	denotes	dilutions
T17411	33118-33120	IN	denotes	of
T17412	33121-33126	NN	denotes	input
T17413	33127-33130	NN	denotes	DNA
T17414	33131-33134	IN	denotes	for
T17415	33135-33139	DT	denotes	each
T17416	33140-33146	NN	denotes	sample
T17417	33146-33147	-COMMA-	denotes	,
T17418	33148-33151	CC	denotes	and
T17419	33152-33153	CD	denotes	1
T17420	33154-33156	NN	denotes	μl
T17421	33157-33161	NN	denotes	ChIP
T17422	33162-33165	NN	denotes	DNA
T17423	33166-33169	VB	denotes	was
T17424	33170-33174	VB	denotes	used
T17425	33175-33178	IN	denotes	per
T17426	33179-33182	NN	denotes	PCR
T17427	33183-33191	NN	denotes	reaction
T17428	33192-33193	-LRB-	denotes	(
T17429	33193-33202	VB	denotes	performed
T17430	33203-33205	IN	denotes	in
T17431	33206-33216	NN	denotes	duplicates
T17432	33216-33217	-RRB-	denotes	)
T17433	33219-33223	NN	denotes	Melt
T17434	33224-33230	NN	denotes	curves
T17435	33231-33234	CC	denotes	and
T17436	33235-33242	NN	denotes	agarose
T17437	33243-33247	NN	denotes	gels
T17438	33248-33252	VB	denotes	were
T17439	33253-33261	VB	denotes	analyzed
T17440	33262-33264	TO	denotes	to
T17441	33265-33271	VB	denotes	ensure
T17442	33272-33285	NN	denotes	amplification
T17443	33286-33288	IN	denotes	of
T17444	33289-33297	JJ	denotes	specific
T17445	33298-33304	NN	denotes	target
T17446	33305-33314	NN	denotes	sequences
T17447	33316-33321	VB	denotes	Refer
T17448	33322-33324	TO	denotes	to
T17449	33325-33330	JJ	denotes	Table
T17450	33331-33333	NN	denotes	S1
T17451	33334-33335	-LRB-	denotes	(
T17452	33335-33344	JJ	denotes	available
T17453	33345-33347	IN	denotes	at
T17454	33348-33352	NN	denotes	http
T17455	33352-33353	-COLON-	denotes	:
T17456	33353-33400	NN	denotes	//www.jem.org/cgi/content/full/jem.20081242/DC1
T17457	33400-33401	-RRB-	denotes	)
T17458	33402-33405	IN	denotes	for
T17459	33406-33407	DT	denotes	a
T17460	33408-33412	NN	denotes	list
T17461	33413-33415	IN	denotes	of
T17462	33416-33422	NN	denotes	primer
T17463	33423-33427	NN	denotes	sets
T17464	33429-33432	DT	denotes	The
T17465	33433-33437	NN	denotes	data
T17466	33438-33441	VB	denotes	are
T17467	33442-33451	VB	denotes	presented
T17468	33452-33454	IN	denotes	as
T17469	33455-33458	DT	denotes	the
T17470	33459-33465	NN	denotes	number
T17471	33466-33468	IN	denotes	of
T17472	33469-33487	VB	denotes	immunoprecipitated
T17473	33488-33494	NN	denotes	target
T17474	33495-33504	NN	denotes	sequences
T17475	33505-33513	JJ	denotes	relative
T17476	33514-33516	TO	denotes	to
T17477	33517-33522	NN	denotes	input
T17478	33523-33532	NN	denotes	chromatin
T17479	33532-33533	-COMMA-	denotes	,
T17480	33534-33542	VB	denotes	assuming
T17481	33543-33546	CD	denotes	two
T17482	33547-33553	NN	denotes	copies
T17483	33554-33556	IN	denotes	of
T17484	33557-33563	NN	denotes	target
T17485	33564-33572	NN	denotes	sequence
T17486	33573-33576	IN	denotes	per
T17487	33577-33581	NN	denotes	cell
T17488	33582-33592	JJ	denotes	equivalent
T17489	33593-33597	VB	denotes	used
T17490	33598-33601	IN	denotes	for
T17491	33602-33605	DT	denotes	the
T17492	33606-33610	NN	denotes	ChIP
T17829	33613-33621	JJ	denotes	Northern
T17830	33622-33625	CC	denotes	and
T17831	33626-33633	JJ	denotes	Western
T17832	33634-33638	NN	denotes	blot
T17833	33639-33647	NN	denotes	analyses
T17834	33649-33652	NN	denotes	RNA
T17835	33653-33662	NN	denotes	isolation
T17836	33663-33666	CC	denotes	and
T17837	33667-33675	JJ	denotes	Northern
T17838	33676-33680	NN	denotes	blot
T17839	33681-33689	NN	denotes	analysis
T17840	33690-33693	VB	denotes	was
T17841	33694-33703	VB	denotes	performed
T17842	33704-33706	IN	denotes	as
T17843	33707-33717	RB	denotes	previously
T17844	33718-33727	VB	denotes	described
T17845	33728-33729	-LRB-	denotes	(
T17846	33729-33731	CD	denotes	29
T17847	33731-33732	-RRB-	denotes	)
T17848	33734-33736	IN	denotes	In
T17849	33737-33742	NN	denotes	brief
T17850	33742-33743	-COMMA-	denotes	,
T17851	33744-33746	CD	denotes	10
T17852	33747-33749	NN	denotes	μg
T17853	33750-33752	IN	denotes	of
T17854	33753-33758	JJ	denotes	total
T17855	33759-33762	NN	denotes	RNA
T17856	33763-33766	VB	denotes	was
T17857	33767-33773	VB	denotes	loaded
T17858	33774-33777	IN	denotes	per
T17859	33778-33782	NN	denotes	lane
T17860	33783-33786	CC	denotes	and
T17861	33787-33798	VB	denotes	transferred
T17862	33799-33801	TO	denotes	to
T17863	33802-33812	RB	denotes	positively
T17864	33813-33820	VB	denotes	charged
T17865	33821-33826	NN	denotes	nylon
T17866	33827-33836	NN	denotes	membranes
T17867	33837-33838	-LRB-	denotes	(
T17868	33838-33847	JJ	denotes	Hybond-N+
T17869	33847-33848	-COLON-	denotes	;
T17870	33849-33851	NNP	denotes	GE
T17871	33852-33862	NNP	denotes	Healthcare
T17872	33862-33863	-RRB-	denotes	)
T17873	33863-33864	-COMMA-	denotes	,
T17874	33865-33870	WDT	denotes	which
T17875	33871-33874	VB	denotes	was
T17876	33875-33884	VB	denotes	confirmed
T17877	33885-33887	IN	denotes	by
T17878	33888-33896	NN	denotes	ethidium
T17879	33897-33904	NN	denotes	bromide
T17880	33905-33913	NN	denotes	staining
T17881	33914-33916	IN	denotes	of
T17882	33917-33926	JJ	denotes	ribosomal
T17883	33927-33930	NN	denotes	RNA
T17884	33931-33938	NN	denotes	species
T17885	33939-33941	IN	denotes	on
T17886	33942-33945	DT	denotes	the
T17887	33946-33954	NN	denotes	membrane
T17888	33956-33965	NN	denotes	Membranes
T17889	33966-33970	VB	denotes	were
T17890	33971-33981	VB	denotes	hybridized
T17891	33982-33986	IN	denotes	with
T17892	33987-33988	CD	denotes	1
T17893	33989-33994	NN	denotes	ng/ml
T17894	33995-33997	SYM	denotes	α-
T17895	33997-33998	-LRB-	denotes	[
T17896	33998-34001	NN	denotes	32P
T17897	34001-34002	-RRB-	denotes	]
T17898	34002-34014	VB	denotes	dCTP–labeled
T17899	34015-34030	JJ	denotes	trichloroacetic
T17900	34031-34035	NN	denotes	acid
T17901	34036-34048	JJ	denotes	precipitable
T17902	34049-34054	NN	denotes	probe
T17903	34055-34057	IN	denotes	in
T17904	34058-34068	NN	denotes	ExpressHyb
T17905	34069-34082	NN	denotes	hybridization
T17906	34083-34089	NN	denotes	buffer
T17907	34090-34091	-LRB-	denotes	(
T17908	34091-34099	NNP	denotes	Clontech
T17909	34100-34112	NNP	denotes	Laboratories
T17910	34112-34113	-COMMA-	denotes	,
T17911	34114-34118	NNP	denotes	Inc.
T17912	34118-34119	-RRB-	denotes	)
T17913	34121-34124	DT	denotes	All
T17914	34125-34129	NN	denotes	cDNA
T17915	34130-34136	NN	denotes	probes
T17916	34137-34141	VB	denotes	were
T17917	34142-34151	VB	denotes	confirmed
T17918	34152-34154	TO	denotes	to
T17919	34155-34159	VB	denotes	have
T17920	34160-34163	DT	denotes	the
T17921	34164-34175	JJ	denotes	appropriate
T17922	34176-34187	JJ	denotes	single-copy
T17923	34188-34199	NN	denotes	specificity
T17924	34200-34205	IN	denotes	under
T17925	34206-34211	DT	denotes	these
T17926	34212-34222	NN	denotes	conditions
T17927	34223-34228	VB	denotes	using
T17928	34229-34236	JJ	denotes	genomic
T17929	34237-34245	JJ	denotes	Southern
T17930	34246-34250	NN	denotes	blot
T17931	34251-34259	NN	denotes	analysis
T17932	34261-34265	NN	denotes	Band
T17933	34266-34277	NN	denotes	intensities
T17934	34278-34282	VB	denotes	were
T17935	34283-34291	VB	denotes	acquired
T17936	34292-34294	IN	denotes	by
T17937	34295-34310	VB	denotes	phosphorimaging
T17938	34311-34319	NN	denotes	analysis
T17939	34321-34324	IN	denotes	For
T17940	34325-34332	JJ	denotes	Western
T17941	34333-34341	NN	denotes	analysis
T17942	34341-34342	-COMMA-	denotes	,
T17943	34343-34353	JJ	denotes	whole-cell
T17944	34354-34361	NN	denotes	protein
T17945	34362-34369	NN	denotes	lysates
T17946	34370-34374	VB	denotes	were
T17947	34375-34383	VB	denotes	obtained
T17948	34384-34388	IN	denotes	from
T17949	34389-34393	JJ	denotes	CD8+
T17950	34394-34395	NN	denotes	T
T17951	34396-34401	NN	denotes	cells
T17952	34402-34404	IN	denotes	at
T17953	34405-34408	DT	denotes	the
T17954	34409-34413	NN	denotes	time
T17955	34414-34420	NN	denotes	points
T17956	34421-34430	VB	denotes	indicated
T17957	34431-34433	IN	denotes	in
T17958	34434-34437	DT	denotes	the
T17959	34438-34445	NN	denotes	figures
T17960	34446-34452	IN	denotes	during
T17961	34453-34459	JJ	denotes	clonal
T17962	34460-34469	NN	denotes	expansion
T17963	34470-34472	IN	denotes	in
T17964	34473-34476	CD	denotes	100
T17965	34477-34481	NN	denotes	U/ml
T17966	34482-34486	NN	denotes	IL-2
T17967	34487-34491	IN	denotes	with
T17968	34492-34497	NN	denotes	lysis
T17969	34498-34504	NN	denotes	buffer
T17970	34505-34506	-LRB-	denotes	(
T17971	34506-34508	CD	denotes	50
T17972	34509-34511	NN	denotes	mM
T17973	34512-34516	NN	denotes	Tris
T17974	34517-34518	-LRB-	denotes	[
T17975	34518-34520	NN	denotes	pH
T17976	34521-34524	CD	denotes	7.5
T17977	34524-34525	-RRB-	denotes	]
T17978	34525-34526	-COMMA-	denotes	,
T17979	34527-34530	CD	denotes	150
T17980	34531-34533	NN	denotes	mM
T17981	34534-34538	NN	denotes	NaCl
T17982	34538-34539	-COMMA-	denotes	,
T17983	34540-34542	CD	denotes	10
T17984	34542-34543	NN	denotes	%
T17985	34544-34552	NN	denotes	glycerol
T17986	34552-34553	-COMMA-	denotes	,
T17987	34554-34555	CD	denotes	5
T17988	34556-34558	NN	denotes	mM
T17989	34559-34563	NN	denotes	EDTA
T17990	34563-34564	-COMMA-	denotes	,
T17991	34565-34566	CD	denotes	1
T17992	34566-34567	NN	denotes	%
T17993	34568-34573	NN	denotes	NP-40
T17994	34573-34574	-RRB-	denotes	)
T17995	34575-34577	IN	denotes	by
T17996	34578-34590	VB	denotes	resuspending
T17997	34591-34598	NN	denotes	samples
T17998	34599-34601	IN	denotes	in
T17999	34602-34604	CD	denotes	10
T18000	34605-34607	NN	denotes	μl
T18001	34608-34611	IN	denotes	per
T18002	34612-34615	CD	denotes	106
T18003	34616-34621	NN	denotes	cells
T18004	34622-34625	CC	denotes	and
T18005	34626-34636	VB	denotes	incubating
T18006	34637-34639	IN	denotes	on
T18007	34640-34643	NN	denotes	ice
T18008	34644-34647	IN	denotes	for
T18009	34648-34650	CD	denotes	30
T18010	34651-34654	NN	denotes	min
T18011	34655-34657	IN	denotes	in
T18012	34658-34661	DT	denotes	the
T18013	34662-34670	NN	denotes	presence
T18014	34671-34673	IN	denotes	of
T18015	34674-34682	NN	denotes	protease
T18016	34683-34693	NN	denotes	inhibitors
T18017	34695-34705	NN	denotes	Immunoblot
T18018	34706-34714	NN	denotes	analysis
T18019	34715-34718	VB	denotes	was
T18020	34719-34728	VB	denotes	performed
T18021	34729-34733	IN	denotes	with
T18022	34734-34737	DT	denotes	the
T18023	34738-34748	NN	denotes	antibodies
T18024	34749-34758	VB	denotes	indicated
T18025	34759-34761	IN	denotes	in
T18026	34762-34765	DT	denotes	the
T18027	34766-34773	NN	denotes	figures
T18028	34774-34779	IN	denotes	after
T18029	34780-34788	NN	denotes	SDS-PAGE
T18030	34789-34790	-LRB-	denotes	(
T18031	34790-34795	CD	denotes	10–30
T18032	34796-34798	NN	denotes	μg
T18033	34799-34801	IN	denotes	of
T18034	34802-34807	JJ	denotes	total
T18035	34808-34815	NN	denotes	protein
T18036	34816-34819	VB	denotes	was
T18037	34820-34826	VB	denotes	loaded
T18038	34827-34830	IN	denotes	per
T18039	34831-34835	RB	denotes	well
T18040	34835-34836	-RRB-	denotes	)
T18041	34838-34852	NN	denotes	Quantification
T18042	34853-34855	IN	denotes	of
T18043	34856-34864	VB	denotes	detected
T18044	34865-34872	NN	denotes	protein
T18045	34873-34876	VB	denotes	was
T18046	34877-34886	VB	denotes	performed
T18047	34887-34891	IN	denotes	with
T18048	34892-34894	DT	denotes	an
T18049	34895-34906	NNP	denotes	Intelligent
T18050	34907-34911	NNP	denotes	Dark
T18051	34912-34915	NNP	denotes	Box
T18052	34916-34920	NN	denotes	unit
T18053	34921-34922	-LRB-	denotes	(
T18054	34922-34930	NN	denotes	LAS-3000
T18055	34930-34931	-COLON-	denotes	;
T18056	34932-34940	NNP	denotes	Fujifilm
T18057	34940-34941	-RRB-	denotes	)
T18058	34942-34945	CC	denotes	and
T18059	34946-34956	VB	denotes	normalized
T18060	34957-34960	IN	denotes	for
T18061	34961-34968	VB	denotes	loading
T18062	34969-34973	IN	denotes	with
T18063	34974-34977	DT	denotes	the
T18064	34978-34984	NN	denotes	amount
T18065	34985-34987	IN	denotes	of
T18066	34988-34991	NN	denotes	RNA
T18067	34992-34998	NN	denotes	Pol-II
T18068	34999-35007	VB	denotes	detected
T18069	35008-35010	IN	denotes	in
T18070	35011-35015	DT	denotes	each
T18071	35016-35020	NN	denotes	lane
T18190	35023-35029	NN	denotes	Online
T18191	35030-35042	JJ	denotes	supplemental
T18192	35043-35051	NN	denotes	material
T18193	35053-35057	NN	denotes	Fig.
T18194	35058-35060	NN	denotes	S1
T18195	35061-35066	VB	denotes	shows
T18196	35067-35070	DT	denotes	the
T18197	35071-35087	NN	denotes	characterization
T18198	35088-35090	IN	denotes	of
T18199	35091-35101	JJ	denotes	peripheral
T18200	35102-35106	JJ	denotes	CD8+
T18201	35107-35108	NN	denotes	T
T18202	35109-35114	NN	denotes	cells
T18203	35115-35119	IN	denotes	from
T18204	35120-35128	NN	denotes	Runx3−/−
T18205	35129-35133	NN	denotes	mice
T18206	35135-35139	NN	denotes	Fig.
T18207	35140-35142	NN	denotes	S2
T18208	35143-35148	VB	denotes	shows
T18209	35149-35157	NN	denotes	effector
T18210	35158-35165	NN	denotes	protein
T18211	35166-35176	NN	denotes	expression
T18212	35177-35179	IN	denotes	by
T18213	35180-35185	NN	denotes	Runx3
T18214	35186-35188	NN	denotes	WT
T18215	35189-35192	CC	denotes	and
T18216	35193-35195	NN	denotes	KO
T18217	35196-35201	NN	denotes	cells
T18218	35202-35204	IN	denotes	at
T18219	35205-35208	NN	denotes	day
T18220	35209-35210	CD	denotes	4
T18221	35211-35213	IN	denotes	of
T18222	35214-35216	FW	denotes	in
T18223	35217-35222	FW	denotes	vitro
T18224	35223-35230	NN	denotes	culture
T18225	35232-35238	NN	denotes	Primer
T18226	35239-35248	NN	denotes	sequences
T18227	35249-35253	VB	denotes	used
T18228	35254-35257	IN	denotes	for
T18229	35258-35262	NN	denotes	ChIP
T18230	35263-35274	NN	denotes	experiments
T18231	35275-35278	VB	denotes	are
T18232	35279-35284	VB	denotes	shown
T18233	35285-35287	IN	denotes	in
T18234	35288-35293	JJ	denotes	Table
T18235	35294-35296	NN	denotes	S1
T18236	35298-35304	NN	denotes	Online
T18237	35305-35317	JJ	denotes	supplemental
T18238	35318-35326	NN	denotes	material
T18239	35327-35329	VB	denotes	is
T18240	35330-35339	JJ	denotes	available
T18241	35340-35342	IN	denotes	at
T18242	35343-35347	NN	denotes	http
T18243	35347-35348	-COLON-	denotes	:
T18244	35348-35395	NN	denotes	//www.jem.org/cgi/content/full/jem.20081242/DC1
R10973	T14019	T14020	arg1Of	Antibodies,and
R10974	T14021	T14020	arg2Of	reagents,and
R10975	T14024	T14022	arg1Of	antibodies,The
R10976	T14024	T14023	arg1Of	antibodies,following
R10977	T14024	T14025	arg2Of	antibodies,used
R10978	T14024	T14031	arg1Of	antibodies,were
R10979	T14024	T14032	arg2Of	antibodies,obtained
R10980	T14025	T14026	arg1Of	used,for
R10981	T14030	T14026	arg2Of	stains,for
R10982	T14030	T14027	arg1Of	stains,intracellular
R10983	T14030	T14028	arg1Of	stains,or
R10984	T14030	T14029	arg1Of	stains,surface
R10985	T14032	T14031	arg2Of	obtained,were
R10986	T14032	T14033	arg1Of	obtained,from
R10987	T14032	T14035	arg1Of	obtained,:
R10988	T14032	T14036	arg1Of	obtained,anti–IL-2
R10989	T14034	T14033	arg2Of	eBioscience,from
R10990	T14036	T14037	arg1Of	anti–IL-2,","
R10991	T14038	T14039	arg1Of	anti–IFN-γ,","
R10992	T14039	T14041	arg1Of	",",","
R10993	T14040	T14039	arg2Of	anti-TNF,","
R10994	T14042	T14041	arg2Of	anti–granzyme,","
R10995	T14043	T14044	arg1Of	B,","
R10996	T14044	T14046	arg1Of	",",","
R10997	T14045	T14044	arg2Of	anti-CD8,","
R10998	T14046	T14049	arg1Of	",",and
R10999	T14047	T14046	arg2Of	anti-CD25,","
R11000	T14049	T14037	arg2Of	and,","
R11001	T14049	T14038	arg1Of	and,anti–IFN-γ
R11002	T14049	T14040	arg1Of	and,anti-TNF
R11003	T14049	T14042	arg1Of	and,anti–granzyme
R11004	T14049	T14048	arg1Of	and,","
R11005	T14050	T14049	arg2Of	anti-CD44,and
R11006	T14051	T14052	arg1Of	Anti-CD69,was
R11007	T14051	T14053	arg2Of	Anti-CD69,purchased
R11008	T14053	T14052	arg2Of	purchased,was
R11009	T14053	T14054	arg1Of	purchased,from
R11010	T14055	T14054	arg2Of	BD,from
R11011	T14058	T14056	arg2Of	experiments,For
R11012	T14058	T14057	arg1Of	experiments,ChIP
R11013	T14062	T14060	arg1Of	antibody,the
R11014	T14062	T14061	arg1Of	antibody,anti-Eomes
R11015	T14062	T14063	arg1Of	antibody,was
R11016	T14062	T14064	arg2Of	antibody,obtained
R11017	T14064	T14063	arg2Of	obtained,was
R11018	T14064	T14065	arg1Of	obtained,from
R11019	T14064	T14067	arg1Of	obtained,and
R11020	T14066	T14065	arg2Of	Abcam,from
R11021	T14067	T14056	arg1Of	and,For
R11022	T14067	T14059	arg1Of	and,","
R11023	T14070	T14068	arg1Of	antibody,the
R11024	T14070	T14069	arg1Of	antibody,anti-Runx3
R11025	T14070	T14071	arg1Of	antibody,was
R11026	T14070	T14072	arg2Of	antibody,produced
R11027	T14072	T14067	arg2Of	produced,and
R11028	T14072	T14071	arg2Of	produced,was
R11029	T14076	T14072	arg1Of	laboratory,produced
R11030	T14076	T14073	arg2Of	laboratory,by
R11031	T14076	T14074	arg1Of	laboratory,the
R11032	T14076	T14075	arg1Of	laboratory,Groner
R11033	T14079	T14077	arg1Of	antibodies,The
R11034	T14079	T14078	arg1Of	antibodies,following
R11035	T14079	T14080	arg1Of	antibodies,were
R11036	T14079	T14081	arg2Of	antibodies,used
R11037	T14081	T14080	arg2Of	used,were
R11038	T14081	T14082	arg1Of	used,for
R11039	T14083	T14082	arg2Of	immunoblotting,for
R11040	T14083	T14084	arg1Of	immunoblotting,:
R11041	T14085	T14086	arg1Of	antiperforin,(
R11042	T14085	T14089	arg1Of	antiperforin,","
R11043	T14087	T14086	arg2Of	Abcam,(
R11044	T14088	T14086	arg3Of	),(
R11045	T14089	T14095	arg1Of	",",and
R11046	T14090	T14089	arg2Of	anti-Eomes,","
R11047	T14090	T14091	arg1Of	anti-Eomes,(
R11048	T14092	T14091	arg2Of	Abcam,(
R11049	T14093	T14091	arg3Of	),(
R11050	T14095	T14084	arg2Of	and,:
R11051	T14095	T14094	arg1Of	and,","
R11052	T14096	T14095	arg2Of	anti–Pol-II,and
R11053	T14096	T14097	arg1Of	anti–Pol-II,(
R11054	T14100	T14097	arg2Of	Biotechnology,(
R11055	T14100	T14098	arg1Of	Biotechnology,Santa
R11056	T14100	T14099	arg1Of	Biotechnology,Cruz
R11057	T14100	T14101	arg1Of	Biotechnology,","
R11058	T14102	T14101	arg2Of	Inc.,","
R11059	T14103	T14097	arg3Of	),(
R11060	T14106	T14104	arg1Of	antibody,The
R11061	T14106	T14105	arg1Of	antibody,T-bet
R11062	T14106	T14107	arg1Of	antibody,was
R11063	T14106	T14108	arg2Of	antibody,provided
R11064	T14108	T14107	arg2Of	provided,was
R11065	T14111	T14108	arg1Of	Glimcher,provided
R11066	T14111	T14109	arg2Of	Glimcher,by
R11067	T14111	T14110	arg1Of	Glimcher,L.
R11068	T14111	T14112	arg1Of	Glimcher,(
R11069	T14114	T14113	arg1Of	School,Harvard
R11070	T14114	T14115	arg1Of	School,of
R11071	T14114	T14118	arg1Of	School,","
R11072	T14117	T14115	arg2Of	Health,of
R11073	T14117	T14116	arg1Of	Health,Public
R11074	T14118	T14112	arg2Of	",",(
R11075	T14118	T14120	arg1Of	",",","
R11076	T14119	T14118	arg2Of	Boston,","
R11077	T14121	T14120	arg2Of	MA,","
R11078	T14122	T14112	arg3Of	),(
R11079	T14125	T14123	arg1Of	reagents,The
R11080	T14125	T14124	arg1Of	reagents,following
R11081	T14125	T14126	arg1Of	reagents,were
R11082	T14125	T14127	arg2Of	reagents,used
R11083	T14127	T14126	arg2Of	used,were
R11084	T14127	T14128	arg1Of	used,for
R11085	T14127	T14135	arg1Of	used,:
R11086	T14130	T14128	arg2Of	experiments,for
R11087	T14130	T14129	arg1Of	experiments,the
R11088	T14130	T14131	arg2Of	experiments,presented
R11089	T14131	T14132	arg1Of	presented,in
R11090	T14134	T14132	arg2Of	report,in
R11091	T14134	T14133	arg1Of	report,this
R11092	T14138	T14137	arg1Of	Apoptosis,V–FITC
R11093	T14140	T14136	arg1Of	Kit,Annexin
R11094	T14140	T14138	arg1Of	Kit,Apoptosis
R11095	T14140	T14139	arg1Of	Kit,Detection
R11096	T14140	T14141	arg1Of	Kit,(
R11097	T14140	T14144	arg1Of	Kit,","
R11098	T14142	T14141	arg2Of	BD,(
R11099	T14143	T14141	arg3Of	),(
R11100	T14145	T14144	arg2Of	CD8,","
R11101	T14148	T14140	arg1Of	Kit,Kit
R11102	T14148	T14145	arg1Of	Kit,CD8
R11103	T14148	T14146	arg1Of	Kit,Negative
R11104	T14148	T14147	arg1Of	Kit,Isolation
R11105	T14148	T14149	arg1Of	Kit,(
R11106	T14148	T14152	arg1Of	Kit,","
R11107	T14150	T14149	arg2Of	Invitrogen,(
R11108	T14151	T14149	arg3Of	),(
R11109	T14152	T14160	arg1Of	",",and
R11110	T14154	T14152	arg2Of	MicroBeads,","
R11111	T14154	T14153	arg1Of	MicroBeads,CD8
R11112	T14154	T14155	arg1Of	MicroBeads,(
R11113	T14157	T14155	arg2Of	Biotec,(
R11114	T14157	T14156	arg1Of	Biotec,Miltenyi
R11115	T14158	T14155	arg3Of	),(
R11116	T14160	T14159	arg1Of	and,","
R11117	T14165	T14160	arg2Of	Reagents,and
R11118	T14165	T14161	arg1Of	Reagents,SYBR
R11119	T14165	T14162	arg1Of	Reagents,Green
R11120	T14165	T14163	arg1Of	Reagents,PCR
R11121	T14165	T14164	arg1Of	Reagents,Core
R11122	T14165	T14166	arg1Of	Reagents,(
R11123	T14168	T14166	arg2Of	Biosystems,(
R11124	T14168	T14167	arg1Of	Biosystems,Applied
R11125	T14169	T14166	arg3Of	),(
R11126	T14172	T14170	arg1Of	peptide,The
R11127	T14172	T14171	arg1Of	peptide,Gp33
R11128	T14172	T14173	arg1Of	peptide,(
R11129	T14172	T14176	arg1Of	peptide,was
R11130	T14172	T14177	arg2Of	peptide,synthesized
R11131	T14174	T14173	arg2Of	KAVYNFATC,(
R11132	T14175	T14173	arg3Of	),(
R11133	T14177	T14176	arg2Of	synthesized,was
R11134	T14177	T14185	arg1Of	synthesized,and
R11135	T14183	T14177	arg1Of	Facility,synthesized
R11136	T14183	T14178	arg2Of	Facility,by
R11137	T14183	T14179	arg1Of	Facility,the
R11138	T14183	T14180	arg1Of	Facility,Tufts
R11139	T14183	T14181	arg1Of	Facility,University
R11140	T14183	T14182	arg1Of	Facility,Core
R11141	T14185	T14184	arg1Of	and,","
R11142	T14187	T14186	arg1Of	mM,10
R11143	T14187	T14188	arg1Of	mM,of
R11144	T14187	T14191	arg1Of	mM,was
R11145	T14187	T14192	arg2Of	mM,prepared
R11146	T14190	T14188	arg2Of	solutions,of
R11147	T14190	T14189	arg1Of	solutions,stock
R11148	T14192	T14185	arg2Of	prepared,and
R11149	T14192	T14191	arg2Of	prepared,was
R11150	T14192	T14193	arg1Of	prepared,in
R11151	T14194	T14193	arg2Of	DMSO,in
R11380	T14607	T14608	arg1Of	Isolation,and
R11381	T14608	T14610	arg1Of	and,of
R11382	T14609	T14608	arg2Of	culture,and
R11383	T14614	T14610	arg2Of	cells,of
R11384	T14614	T14611	arg1Of	cells,primary
R11385	T14614	T14612	arg1Of	cells,CD8+
R11386	T14614	T14613	arg1Of	cells,T
R11387	T14617	T14615	arg1Of	cells,CD8+
R11388	T14617	T14616	arg1Of	cells,T
R11389	T14617	T14618	arg1Of	cells,from
R11390	T14617	T14624	arg1Of	cells,transgenic
R11391	T14617	T14628	arg1Of	cells,","
R11392	T14623	T14618	arg2Of	TCR,from
R11393	T14623	T14619	arg1Of	TCR,4–8-wk-old
R11394	T14623	T14620	arg1Of	TCR,Tcra−/−
R11395	T14623	T14621	arg1Of	TCR,×
R11396	T14623	T14622	arg1Of	TCR,P14
R11397	T14624	T14625	arg1Of	transgenic,(
R11398	T14626	T14625	arg2Of	Taconic,(
R11399	T14627	T14625	arg3Of	),(
R11400	T14628	T14632	arg1Of	",",or
R11401	T14630	T14628	arg2Of	WT,","
R11402	T14630	T14629	arg1Of	WT,C57BL/6J
R11403	T14632	T14631	arg1Of	or,","
R11404	T14632	T14640	arg1Of	or,were
R11405	T14632	T14641	arg2Of	or,purified
R11406	T14633	T14634	arg1Of	Tbx21−/−,(
R11407	T14637	T14634	arg2Of	Laboratory,(
R11408	T14637	T14635	arg1Of	Laboratory,The
R11409	T14637	T14636	arg1Of	Laboratory,Jackson
R11410	T14638	T14634	arg3Of	),(
R11411	T14639	T14632	arg2Of	mice,or
R11412	T14639	T14633	arg1Of	mice,Tbx21−/−
R11413	T14641	T14640	arg2Of	purified,were
R11414	T14641	T14642	arg1Of	purified,(
R11415	T14641	T14648	arg1Of	purified,by
R11416	T14641	T14654	arg1Of	purified,from
R11417	T14644	T14643	arg1Of	95,>
R11418	T14644	T14645	arg1Of	95,%
R11419	T14646	T14642	arg2Of	purity,(
R11420	T14646	T14644	arg1Of	purity,95
R11421	T14647	T14642	arg3Of	),(
R11422	T14650	T14648	arg2Of	selection,by
R11423	T14650	T14649	arg1Of	selection,negative
R11424	T14650	T14651	arg1Of	selection,(
R11425	T14652	T14651	arg2Of	Invitrogen,(
R11426	T14653	T14651	arg3Of	),(
R11427	T14656	T14657	arg1Of	spleen,and
R11428	T14658	T14657	arg2Of	lymph,and
R11429	T14660	T14654	arg2Of	cells,from
R11430	T14660	T14655	arg2Of	cells,pooled
R11431	T14660	T14656	arg1Of	cells,spleen
R11432	T14660	T14658	arg1Of	cells,lymph
R11433	T14660	T14659	arg1Of	cells,node
R11434	T14663	T14661	arg1Of	cells,CD8+
R11435	T14663	T14662	arg1Of	cells,T
R11436	T14663	T14664	arg1Of	cells,from
R11437	T14663	T14671	arg1Of	cells,were
R11438	T14663	T14672	arg2Of	cells,purified
R11439	T14666	T14664	arg2Of	mice,from
R11440	T14666	T14665	arg1Of	mice,Runx3−/−
R11441	T14666	T14667	arg1Of	mice,on
R11442	T14670	T14667	arg2Of	background,on
R11443	T14670	T14668	arg1Of	background,the
R11444	T14670	T14669	arg1Of	background,ICR
R11445	T14672	T14671	arg2Of	purified,were
R11446	T14675	T14672	arg1Of	selection,purified
R11447	T14675	T14673	arg2Of	selection,by
R11448	T14675	T14674	arg1Of	selection,positive
R11449	T14675	T14676	arg1Of	selection,(
R11450	T14678	T14676	arg2Of	Biotec,(
R11451	T14678	T14677	arg1Of	Biotec,Miltenyi
R11452	T14679	T14676	arg3Of	),(
R11453	T14681	T14680	arg1Of	mice,All
R11454	T14681	T14682	arg1Of	mice,were
R11455	T14681	T14683	arg2Of	mice,maintained
R11456	T14681	T14690	arg2Of	mice,used
R11457	T14683	T14684	arg1Of	maintained,in
R11458	T14683	T14689	arg1Of	maintained,and
R11459	T14688	T14684	arg2Of	facilities,in
R11460	T14688	T14685	arg1Of	facilities,specific
R11461	T14688	T14686	arg1Of	facilities,pathogen-free
R11462	T14688	T14687	arg1Of	facilities,barrier
R11463	T14689	T14682	arg2Of	and,were
R11464	T14689	T14712	arg1Of	and,","
R11465	T14690	T14689	arg2Of	used,and
R11466	T14690	T14691	arg1Of	used,according
R11467	T14690	T14710	arg1Of	used,For
R11468	T14692	T14691	arg2Of	to,according
R11469	T14693	T14694	arg2Of	protocols,approved
R11470	T14693	T14700	arg1Of	protocols,and
R11471	T14699	T14694	arg1Of	Institute,approved
R11472	T14699	T14695	arg2Of	Institute,by
R11473	T14699	T14696	arg1Of	Institute,the
R11474	T14699	T14697	arg1Of	Institute,Immune
R11475	T14699	T14698	arg1Of	Institute,Disease
R11476	T14700	T14692	arg2Of	and,to
R11477	T14706	T14702	arg1Of	Care,Harvard
R11478	T14706	T14703	arg1Of	Care,Medical
R11479	T14706	T14704	arg1Of	Care,School
R11480	T14706	T14705	arg1Of	Care,Animal
R11481	T14706	T14707	arg1Of	Care,and
R11482	T14708	T14707	arg2Of	Use,and
R11483	T14709	T14700	arg2Of	Committees.,and
R11484	T14709	T14701	arg1Of	Committees.,the
R11485	T14709	T14706	arg1Of	Committees.,Care
R11486	T14709	T14708	arg1Of	Committees.,Use
R11487	T14711	T14710	arg2Of	stimulation,For
R11488	T14716	T14713	arg2Of	cells,purified
R11489	T14716	T14714	arg1Of	cells,CD8+
R11490	T14716	T14715	arg1Of	cells,T
R11491	T14716	T14717	arg1Of	cells,were
R11492	T14716	T14718	arg2Of	cells,cultured
R11493	T14718	T14712	arg2Of	cultured,","
R11494	T14718	T14717	arg2Of	cultured,were
R11495	T14718	T14719	arg1Of	cultured,at
R11496	T14721	T14719	arg2Of	cells/ml,at
R11497	T14721	T14720	arg1Of	cells/ml,106
R11498	T14721	T14722	arg1Of	cells/ml,(
R11499	T14721	T14726	arg1Of	cells/ml,in
R11500	T14724	T14722	arg2Of	ml,(
R11501	T14724	T14723	arg1Of	ml,10
R11502	T14725	T14722	arg3Of	),(
R11503	T14728	T14726	arg2Of	flasks,in
R11504	T14728	T14727	arg1Of	flasks,T25
R11505	T14728	T14729	arg2Of	flasks,coated
R11506	T14729	T14730	arg1Of	coated,with
R11507	T14732	T14730	arg2Of	μg/ml,with
R11508	T14732	T14731	arg1Of	μg/ml,1
R11509	T14732	T14733	arg1Of	μg/ml,each
R11510	T14732	T14734	arg1Of	μg/ml,of
R11511	T14735	T14736	arg1Of	anti-CD3,(
R11512	T14735	T14740	arg1Of	anti-CD3,and
R11513	T14738	T14736	arg2Of	2C11,(
R11514	T14738	T14737	arg1Of	2C11,clone
R11515	T14739	T14736	arg3Of	),(
R11516	T14740	T14734	arg2Of	and,of
R11517	T14740	T14746	arg1Of	and,by
R11518	T14743	T14740	arg2Of	clone,and
R11519	T14743	T14741	arg1Of	clone,anti-CD28
R11520	T14743	T14742	arg2Of	clone,(
R11521	T14743	T14744	arg1Of	clone,37.51
R11522	T14745	T14742	arg3Of	),(
R11523	T14747	T14746	arg2Of	pretreatment,by
R11524	T14747	T14748	arg1Of	pretreatment,with
R11525	T14753	T14748	arg2Of	IgG,with
R11526	T14753	T14749	arg1Of	IgG,300
R11527	T14753	T14750	arg1Of	IgG,μg/ml
R11528	T14753	T14751	arg1Of	IgG,goat
R11529	T14753	T14752	arg1Of	IgG,anti–hamster
R11530	T14756	T14754	arg2Of	h,After
R11531	T14756	T14755	arg1Of	h,48
R11532	T14758	T14759	arg1Of	cells,were
R11533	T14758	T14760	arg2Of	cells,removed
R11534	T14758	T14766	arg2Of	cells,recultured
R11535	T14760	T14761	arg1Of	removed,from
R11536	T14760	T14765	arg1Of	removed,and
R11537	T14764	T14761	arg2Of	stimulation,from
R11538	T14764	T14762	arg1Of	stimulation,the
R11539	T14764	T14763	arg1Of	stimulation,TCR
R11540	T14765	T14754	arg1Of	and,After
R11541	T14765	T14757	arg1Of	and,","
R11542	T14765	T14759	arg2Of	and,were
R11543	T14766	T14765	arg2Of	recultured,and
R11544	T14766	T14767	arg1Of	recultured,at
R11545	T14769	T14767	arg2Of	concentration,at
R11546	T14769	T14768	arg1Of	concentration,a
R11547	T14769	T14770	arg1Of	concentration,of
R11548	T14769	T14775	arg1Of	concentration,in
R11549	T14773	T14772	arg1Of	105,×
R11550	T14774	T14770	arg2Of	cells/ml,of
R11551	T14774	T14771	arg1Of	cells/ml,5
R11552	T14774	T14773	arg1Of	cells/ml,105
R11553	T14776	T14775	arg2Of	media,in
R11554	T14776	T14777	arg2Of	media,supplemented
R11555	T14777	T14778	arg1Of	supplemented,with
R11556	T14781	T14778	arg2Of	rhIL-2,with
R11557	T14781	T14779	arg1Of	rhIL-2,100
R11558	T14781	T14780	arg1Of	rhIL-2,U/ml
R11559	T14784	T14782	arg1Of	h,Every
R11560	T14784	T14783	arg1Of	h,24
R11561	T14787	T14786	arg1Of	cells,viable
R11562	T14787	T14788	arg1Of	cells,were
R11563	T14787	T14789	arg2Of	cells,counted
R11564	T14787	T14791	arg2Of	cells,readjusted
R11565	T14789	T14790	arg1Of	counted,and
R11566	T14790	T14784	arg1Of	and,h
R11567	T14790	T14785	arg1Of	and,","
R11568	T14790	T14788	arg2Of	and,were
R11569	T14791	T14790	arg2Of	readjusted,and
R11570	T14791	T14792	arg1Of	readjusted,to
R11571	T14795	T14794	arg1Of	105,×
R11572	T14796	T14792	arg2Of	cells/ml,to
R11573	T14796	T14793	arg1Of	cells/ml,5
R11574	T14796	T14795	arg1Of	cells/ml,105
R11575	T14796	T14797	arg1Of	cells/ml,with
R11576	T14799	T14797	arg2Of	media,with
R11577	T14799	T14798	arg1Of	media,fresh
R11578	T14799	T14800	arg1Of	media,containing
R11579	T14803	T14800	arg2Of	amount,containing
R11580	T14803	T14801	arg1Of	amount,the
R11581	T14803	T14802	arg1Of	amount,corresponding
R11582	T14803	T14804	arg1Of	amount,of
R11583	T14805	T14804	arg2Of	rhIL-2,of
R11973	T15411	T15412	arg1Of	Isolation,of
R11974	T15415	T15412	arg2Of	cells,of
R11975	T15415	T15413	arg1Of	cells,CD8+
R11976	T15415	T15414	arg1Of	cells,T
R11977	T15415	T15416	arg1Of	cells,from
R11978	T15418	T15416	arg2Of	mice,from
R11979	T15418	T15417	arg1Of	mice,Runx3−/−
R11980	T15421	T15419	arg1Of	cells,Runx3-deficient
R11981	T15421	T15420	arg1Of	cells,T
R11982	T15421	T15422	arg1Of	cells,fail
R11983	T15422	T15423	arg1Of	fail,to
R11984	T15422	T15432	arg1Of	fail,(
R11985	T15426	T15423	arg2Of	expression,to
R11986	T15426	T15424	arg1Of	expression,silence
R11987	T15426	T15425	arg1Of	expression,CD4
R11988	T15426	T15427	arg1Of	expression,normally
R11989	T15426	T15428	arg1Of	expression,(
R11990	T15430	T15428	arg2Of	S1,(
R11991	T15430	T15429	arg1Of	S1,Fig.
R11992	T15431	T15428	arg3Of	),(
R11993	T15433	T15432	arg2Of	12,(
R11994	T15433	T15434	arg1Of	12,","
R11995	T15435	T15434	arg2Of	13,","
R11996	T15436	T15432	arg3Of	),(
R11997	T15437	T15440	arg1Of	We,fractionated
R11998	T15440	T15438	arg1Of	fractionated,therefore
R11999	T15440	T15439	arg1Of	fractionated,further
R12000	T15440	T15451	arg1Of	fractionated,into
R12001	T15440	T15460	modOf	fractionated,using
R12002	T15443	T15442	arg1Of	selected,positively
R12003	T15446	T15440	arg2Of	cells,fractionated
R12004	T15446	T15441	arg1Of	cells,the
R12005	T15446	T15443	arg1Of	cells,selected
R12006	T15446	T15444	arg1Of	cells,CD8+
R12007	T15446	T15445	arg1Of	cells,T
R12008	T15446	T15447	arg1Of	cells,from
R12009	T15450	T15447	arg2Of	mice,from
R12010	T15450	T15448	arg1Of	mice,Runx3
R12011	T15450	T15449	arg1Of	mice,KO
R12012	T15453	T15452	arg1Of	SP,CD8+CD4−
R12013	T15453	T15454	arg1Of	SP,or
R12014	T15454	T15451	arg2Of	or,into
R12015	T15454	T15458	arg1Of	or,by
R12016	T15457	T15454	arg2Of	cells,or
R12017	T15457	T15455	arg1Of	cells,CD8+CD4+
R12018	T15457	T15456	arg1Of	cells,DP
R12019	T15459	T15458	arg2Of	separation,by
R12020	T15462	T15461	arg1Of	magnetic,anti-CD4
R12021	T15463	T15460	arg2Of	beads,using
R12022	T15463	T15462	arg1Of	beads,magnetic
R12023	T15464	T15465	arg1Of	This,yielded
R12024	T15465	T15478	arg1Of	yielded,and
R12025	T15469	T15466	arg1Of	SP,a
R12026	T15469	T15467	arg1Of	SP,Runx3
R12027	T15469	T15468	arg1Of	SP,KO
R12028	T15469	T15470	arg1Of	SP,“enriched”
R12029	T15471	T15470	arg2Of	population,“enriched”
R12030	T15472	T15465	arg2Of	that,yielded
R12031	T15472	T15473	arg1Of	that,contained
R12032	T15473	T15470	arg3Of	contained,“enriched”
R12033	T15474	T15475	arg1Of	75,%
R12034	T15477	T15473	arg2Of	cells,contained
R12035	T15477	T15474	arg1Of	cells,75
R12036	T15477	T15476	arg1Of	cells,CD8+CD4−
R12037	T15481	T15479	arg1Of	DP,a
R12038	T15481	T15480	arg1Of	DP,KO
R12039	T15481	T15482	arg1Of	DP,enriched
R12040	T15482	T15478	arg2Of	enriched,and
R12041	T15483	T15482	arg2Of	population,enriched
R12042	T15483	T15484	arg1Of	population,that
R12043	T15483	T15485	arg1Of	population,contained
R12044	T15486	T15487	arg1Of	85,%
R12045	T15489	T15485	arg2Of	cells,contained
R12046	T15489	T15486	arg1Of	cells,85
R12047	T15489	T15488	arg1Of	cells,CD8+CD4+
R12048	T15489	T15490	arg1Of	cells,(
R12049	T15492	T15490	arg2Of	S1,(
R12050	T15492	T15491	arg1Of	S1,Fig.
R12051	T15493	T15490	arg3Of	),(
R12052	T15495	T15494	arg1Of	cells,The
R12053	T15495	T15496	arg1Of	cells,were
R12054	T15495	T15497	arg2Of	cells,stimulated
R12055	T15495	T15505	arg1Of	cells,removing
R12056	T15495	T15512	arg1Of	cells,culturing
R12057	T15497	T15496	arg2Of	stimulated,were
R12058	T15497	T15498	arg1Of	stimulated,with
R12059	T15497	T15501	arg1Of	stimulated,for
R12060	T15497	T15504	arg1Of	stimulated,before
R12061	T15500	T15498	arg2Of	anti-CD28,with
R12062	T15500	T15499	arg1Of	anti-CD28,anti-CD3+
R12063	T15503	T15501	arg2Of	d,for
R12064	T15503	T15502	arg1Of	d,2
R12065	T15505	T15507	arg1Of	removing,from
R12066	T15505	T15511	arg1Of	removing,and
R12067	T15506	T15505	arg2Of	them,removing
R12068	T15510	T15507	arg2Of	stimulus,from
R12069	T15510	T15508	arg1Of	stimulus,the
R12070	T15510	T15509	arg1Of	stimulus,TCR
R12071	T15511	T15504	arg2Of	and,before
R12072	T15512	T15511	arg2Of	culturing,and
R12073	T15512	T15514	arg1Of	culturing,in
R12074	T15513	T15512	arg2Of	them,culturing
R12075	T15515	T15514	arg2Of	media,in
R12076	T15515	T15516	arg1Of	media,containing
R12077	T15519	T15516	arg2Of	IL-2,containing
R12078	T15519	T15517	arg1Of	IL-2,100
R12079	T15519	T15518	arg1Of	IL-2,U/ml
R12080	T15522	T15520	arg2Of	reported,As
R12081	T15522	T15521	arg1Of	reported,previously
R12082	T15525	T15524	arg1Of	proliferation,TCR-induced
R12083	T15525	T15526	arg1Of	proliferation,of
R12084	T15525	T15531	arg1Of	proliferation,was
R12085	T15525	T15533	arg1Of	proliferation,impaired
R12086	T15525	T15535	arg1Of	proliferation,irrespective
R12087	T15530	T15526	arg2Of	cells,of
R12088	T15530	T15527	arg1Of	cells,Runx3−/−
R12089	T15530	T15528	arg1Of	cells,CD8+
R12090	T15530	T15529	arg1Of	cells,T
R12091	T15531	T15520	arg1Of	was,As
R12092	T15531	T15523	arg1Of	was,","
R12093	T15531	T15543	arg1Of	was,(
R12094	T15533	T15534	arg1Of	impaired,","
R12095	T15534	T15531	arg2Of	",",was
R12096	T15534	T15532	arg1Of	",",severely
R12097	T15535	T15534	arg2Of	irrespective,","
R12098	T15535	T15536	arg1Of	irrespective,of
R12099	T15538	T15536	arg2Of	expression,of
R12100	T15538	T15537	arg1Of	expression,CD4
R12101	T15538	T15539	arg1Of	expression,(
R12102	T15541	T15539	arg2Of	S1,(
R12103	T15541	T15540	arg1Of	S1,Fig.
R12104	T15542	T15539	arg3Of	),(
R12105	T15544	T15543	arg2Of	12,(
R12106	T15544	T15545	arg1Of	12,","
R12107	T15546	T15545	arg2Of	13,","
R12108	T15547	T15543	arg3Of	),(
R12109	T15552	T15550	arg1Of	cells,the
R12110	T15552	T15551	arg1Of	cells,Runx3−/−
R12111	T15552	T15553	arg1Of	cells,showed
R12112	T15553	T15548	arg1Of	showed,However
R12113	T15553	T15549	arg1Of	showed,","
R12114	T15556	T15553	arg2Of	patterns,showed
R12115	T15556	T15554	arg1Of	patterns,cell-surface
R12116	T15556	T15555	arg1Of	patterns,expression
R12117	T15556	T15557	arg1Of	patterns,indicative
R12118	T15556	T15561	arg1Of	patterns,","
R12119	T15556	T15562	arg1Of	patterns,including
R12120	T15557	T15558	arg1Of	indicative,of
R12121	T15560	T15558	arg2Of	cells,of
R12122	T15560	T15559	arg2Of	cells,activated
R12123	T15563	T15564	arg1Of	up-regulation,of
R12124	T15563	T15566	arg1Of	up-regulation,and
R12125	T15565	T15564	arg2Of	CD25,of
R12126	T15566	T15562	arg2Of	and,including
R12127	T15567	T15566	arg2Of	CD69,and
R12128	T15567	T15568	arg1Of	CD69,(
R12129	T15570	T15568	arg2Of	S1,(
R12130	T15570	T15569	arg1Of	S1,Fig.
R12131	T15571	T15568	arg3Of	),(
R12132	T15573	T15572	arg2Of	expected,As
R12133	T15573	T15574	arg1Of	expected,from
R12134	T15576	T15574	arg2Of	ability,from
R12135	T15576	T15575	arg1Of	ability,their
R12136	T15576	T15577	modOf	ability,to
R12137	T15578	T15577	arg1Of	up-regulate,to
R12138	T15579	T15578	arg2Of	CD25,up-regulate
R12139	T15584	T15581	arg1Of	cells,Runx3−/−
R12140	T15584	T15582	arg1Of	cells,CD8+
R12141	T15584	T15583	arg1Of	cells,T
R12142	T15584	T15585	arg1Of	cells,responded
R12143	T15584	T15594	arg2Of	cells,expanded
R12144	T15585	T15586	arg1Of	responded,to
R12145	T15585	T15589	arg1Of	responded,after
R12146	T15585	T15592	arg1Of	responded,and
R12147	T15588	T15586	arg2Of	supplementation,to
R12148	T15588	T15587	arg1Of	supplementation,IL-2
R12149	T15590	T15589	arg2Of	day,after
R12150	T15590	T15591	arg1Of	day,2
R12151	T15592	T15572	arg1Of	and,As
R12152	T15592	T15580	arg1Of	and,","
R12153	T15594	T15592	arg2Of	expanded,and
R12154	T15594	T15593	arg1Of	expanded,efficiently
R12155	T15594	T15595	arg1Of	expanded,until
R12156	T15594	T15602	arg1Of	expanded,","
R12157	T15594	T15604	arg1Of	expanded,at
R12158	T15596	T15595	arg2Of	day,until
R12159	T15596	T15597	arg1Of	day,6
R12160	T15596	T15598	arg1Of	day,of
R12161	T15601	T15598	arg2Of	period,of
R12162	T15601	T15599	arg1Of	period,the
R12163	T15601	T15600	arg1Of	period,culture
R12164	T15604	T15603	arg1Of	at,albeit
R12165	T15606	T15604	arg2Of	rates,at
R12166	T15606	T15605	arg1Of	rates,slower
R12167	T15606	T15607	arg1Of	rates,compared
R12168	T15608	T15607	arg2Of	with,compared
R12169	T15610	T15608	arg2Of	cells,with
R12170	T15610	T15609	arg1Of	cells,WT
R12171	T15610	T15611	arg1Of	cells,(
R12172	T15613	T15611	arg2Of	S1,(
R12173	T15613	T15612	arg1Of	S1,Fig.
R12174	T15614	T15611	arg3Of	),(
R12175	T15617	T15616	arg1Of	fraction,a
R12176	T15617	T15618	arg1Of	fraction,of
R12177	T15617	T15623	arg1Of	fraction,silenced
R12178	T15622	T15618	arg2Of	cells,of
R12179	T15622	T15619	arg1Of	cells,the
R12180	T15622	T15620	arg1Of	cells,KO
R12181	T15622	T15621	arg1Of	cells,DP
R12182	T15623	T15615	arg2Of	silenced,Although
R12183	T15623	T15626	arg1Of	silenced,after
R12184	T15625	T15623	arg2Of	expression,silenced
R12185	T15625	T15624	arg1Of	expression,CD4
R12186	T15627	T15626	arg2Of	activation,after
R12187	T15630	T15629	arg1Of	ratio,the
R12188	T15630	T15631	arg1Of	ratio,of
R12189	T15630	T15634	arg1Of	ratio,in
R12190	T15630	T15638	arg1Of	ratio,remained
R12191	T15630	T15639	arg1Of	ratio,constant
R12192	T15633	T15631	arg2Of	cells,of
R12193	T15633	T15632	arg1Of	cells,SP/DP
R12194	T15637	T15634	arg2Of	population,in
R12195	T15637	T15635	arg1Of	population,each
R12196	T15637	T15636	arg2Of	population,enriched
R12197	T15638	T15615	arg1Of	remained,Although
R12198	T15638	T15628	arg1Of	remained,","
R12199	T15638	T15640	arg1Of	remained,thereafter
R12200	T15638	T15642	arg1Of	remained,and
R12201	T15639	T15638	arg2Of	constant,remained
R12202	T15642	T15641	arg1Of	and,","
R12203	T15643	T15644	arg1Of	we,did
R12204	T15643	T15646	arg1Of	we,observe
R12205	T15646	T15642	arg2Of	observe,and
R12206	T15646	T15644	arg2Of	observe,did
R12207	T15646	T15645	arg1Of	observe,not
R12208	T15646	T15658	arg1Of	observe,","
R12209	T15646	T15659	modOf	observe,indicating
R12210	T15649	T15646	arg2Of	differences,observe
R12211	T15649	T15647	arg1Of	differences,any
R12212	T15649	T15648	arg1Of	differences,major
R12213	T15649	T15650	arg1Of	differences,between
R12214	T15653	T15650	arg2Of	populations,between
R12215	T15653	T15651	arg1Of	populations,these
R12216	T15653	T15652	arg1Of	populations,two
R12217	T15653	T15654	arg1Of	populations,throughout
R12218	T15657	T15654	arg2Of	period,throughout
R12219	T15657	T15655	arg1Of	period,the
R12220	T15657	T15656	arg1Of	period,culture
R12221	T15661	T15667	arg1Of	in,and
R12222	T15662	T15661	arg2Of	terms,in
R12223	T15662	T15663	arg1Of	terms,of
R12224	T15666	T15663	arg2Of	differentiation,of
R12225	T15666	T15664	arg1Of	differentiation,effector
R12226	T15666	T15665	arg1Of	differentiation,CTL
R12227	T15668	T15667	arg2Of	under,and
R12228	T15671	T15668	arg2Of	conditions,under
R12229	T15671	T15669	arg1Of	conditions,our
R12230	T15671	T15670	arg1Of	conditions,culture
R12231	T15676	T15673	arg1Of	cells,Runx3−/−
R12232	T15676	T15674	arg1Of	cells,CD8+
R12233	T15676	T15675	arg1Of	cells,T
R12234	T15676	T15677	arg1Of	cells,that
R12235	T15676	T15679	arg1Of	cells,coexpress
R12236	T15676	T15681	arg1Of	cells,are
R12237	T15676	T15682	arg1Of	cells,indistinguishable
R12238	T15679	T15678	arg1Of	coexpress,also
R12239	T15680	T15679	arg2Of	CD4,coexpress
R12240	T15681	T15659	arg2Of	are,indicating
R12241	T15681	T15660	arg1Of	are,that
R12242	T15681	T15661	arg1Of	are,in
R12243	T15681	T15668	arg1Of	are,under
R12244	T15681	T15672	arg1Of	are,","
R12245	T15682	T15681	arg2Of	indistinguishable,are
R12246	T15682	T15683	arg1Of	indistinguishable,from
R12247	T15684	T15683	arg2Of	those,from
R12248	T15684	T15685	arg1Of	those,that
R12249	T15684	T15686	arg1Of	those,do
R12250	T15686	T15687	arg1Of	do,not
R12251	T15689	T15688	arg1Of	data,The
R12252	T15689	T15690	arg2Of	data,presented
R12253	T15689	T15694	arg1Of	data,are
R12254	T15689	T15709	arg1Of	data,are
R12255	T15689	T15710	arg1Of	data,from
R12256	T15690	T15691	arg1Of	presented,in
R12257	T15693	T15691	arg2Of	S2,in
R12258	T15693	T15692	arg1Of	S2,Fig.
R12259	T15694	T15695	arg1Of	are,from
R12260	T15694	T15700	arg1Of	are,","
R12261	T15694	T15701	arg1Of	are,whereas
R12262	T15699	T15695	arg2Of	cells,from
R12263	T15699	T15696	arg1Of	cells,Runx3
R12264	T15699	T15697	arg1Of	cells,KO
R12265	T15699	T15698	arg1Of	cells,SP
R12266	T15702	T15703	arg2Of	those,shown
R12267	T15703	T15704	arg1Of	shown,in
R12268	T15705	T15706	arg1Of	Figs.,3
R12269	T15705	T15707	arg1Of	Figs.,and
R12270	T15707	T15704	arg2Of	and,in
R12271	T15708	T15707	arg2Of	4,and
R12272	T15709	T15701	arg2Of	are,whereas
R12273	T15709	T15703	modOf	are,shown
R12274	T15710	T15694	arg2Of	from,are
R12275	T15710	T15709	arg2Of	from,are
R12276	T15715	T15710	arg2Of	cells,from
R12277	T15715	T15711	arg1Of	cells,total
R12278	T15715	T15712	arg1Of	cells,Runx3
R12279	T15715	T15713	arg1Of	cells,KO
R12280	T15715	T15714	arg1Of	cells,CD8
R1230	T1505	T1503	arg1Of	cells,Naive
R1231	T1505	T1504	arg1Of	cells,CD8+ T
R1232	T1505	T1506	arg1Of	cells,differentiate
R1233	T1506	T1507	arg1Of	differentiate,into
R1234	T1506	T1510	arg1Of	differentiate,with
R1235	T1509	T1507	arg2Of	CTLs,into
R1236	T1509	T1508	arg1Of	CTLs,effector
R1237	T1512	T1510	arg2Of	ability,with
R1238	T1512	T1511	arg1Of	ability,the
R1239	T1512	T1513	modOf	ability,to
R1240	T1512	T1529	modOf	ability,to
R1241	T1514	T1513	arg1Of	lyse,to
R1242	T1514	T1518	arg1Of	lyse,by
R1243	T1514	T1528	arg1Of	lyse,and
R1244	T1517	T1514	arg2Of	cells,lyse
R1245	T1517	T1515	arg1Of	cells,antigen-bearing
R12451	T16231	T16229	arg1Of	assay,FACS-based
R12452	T16231	T16230	arg1Of	assay,cytotoxicity
R12453	T16233	T16232	arg1Of	measure,To
R12454	T16234	T16233	arg2Of	cytotoxicity,measure
R12455	T16239	T16233	arg1Of	cells,measure
R12456	T16239	T16236	arg1Of	cells,EL4
R12457	T16239	T16237	arg1Of	cells,thymoma
R12458	T16239	T16238	arg1Of	cells,target
R12459	T16239	T16240	arg1Of	cells,were
R1246	T1517	T1516	arg1Of	cells,target
R12460	T16239	T16241	arg2Of	cells,loaded
R12461	T16241	T16232	modOf	loaded,To
R12462	T16241	T16235	arg1Of	loaded,","
R12463	T16241	T16240	arg2Of	loaded,were
R12464	T16241	T16242	arg1Of	loaded,with
R12465	T16241	T16252	arg1Of	loaded,before
R12466	T16241	T16261	arg1Of	loaded,at
R12467	T16243	T16244	arg1Of	0,or
R12468	T16244	T16246	arg1Of	or,μM
R12469	T16245	T16244	arg2Of	1,or
R1247	T1519	T1518	arg2Of	exocytosis,by
R12470	T16248	T16242	arg2Of	peptide,with
R12471	T16248	T16243	arg1Of	peptide,0
R12472	T16248	T16245	arg1Of	peptide,1
R12473	T16248	T16247	arg1Of	peptide,Gp33
R12474	T16248	T16249	arg1Of	peptide,for
R12475	T16251	T16249	arg2Of	h,for
R12476	T16251	T16250	arg1Of	h,2
R12477	T16255	T16252	arg2Of	coincubation,before
R12478	T16255	T16253	arg1Of	coincubation,a
R12479	T16255	T16254	arg1Of	coincubation,2-h
R1248	T1519	T1520	arg1Of	exocytosis,of
R12480	T16255	T16256	arg1Of	coincubation,with
R12481	T16260	T16256	arg2Of	cells,with
R12482	T16260	T16257	arg1Of	cells,P14
R12483	T16260	T16258	arg1Of	cells,CD8+
R12484	T16260	T16259	arg1Of	cells,T
R12485	T16264	T16261	arg2Of	ratios,at
R12486	T16264	T16262	arg1Of	ratios,the
R12487	T16264	T16263	arg1Of	ratios,effector-to-target
R12488	T16264	T16265	arg2Of	ratios,indicated
R12489	T16265	T16266	arg1Of	indicated,in
R1249	T1522	T1520	arg2Of	granules,of
R12490	T16268	T16266	arg2Of	figures,in
R12491	T16268	T16267	arg1Of	figures,the
R12492	T16268	T16269	arg1Of	figures,in
R12493	T16272	T16269	arg2Of	plates,in
R12494	T16272	T16270	arg1Of	plates,96-well
R12495	T16272	T16271	arg1Of	plates,round-bottom
R12496	T16276	T16273	arg2Of	period,After
R12497	T16276	T16274	arg1Of	period,the
R12498	T16276	T16275	arg1Of	period,coincubation
R12499	T16278	T16279	arg1Of	cells,were
R1250	T1522	T1521	arg1Of	granules,lytic
R12500	T16278	T16280	arg2Of	cells,stained
R12501	T16280	T16273	arg1Of	stained,After
R12502	T16280	T16277	arg1Of	stained,","
R12503	T16280	T16279	arg2Of	stained,were
R12504	T16280	T16281	arg1Of	stained,with
R12505	T16283	T16284	arg1Of	V–FITC,and
R12506	T16284	T16281	arg2Of	and,with
R12507	T16284	T16282	arg1Of	and,Annexin
R12508	T16285	T16284	arg2Of	anti-CD8–allophycocyanin,and
R12509	T16287	T16286	arg1Of	analysis,Data
R1251	T1522	T1523	arg1Of	granules,containing
R12510	T16287	T16288	arg1Of	analysis,was
R12511	T16287	T16289	arg2Of	analysis,performed
R12512	T16289	T16288	arg2Of	performed,was
R12513	T16289	T16290	arg1Of	performed,with
R12514	T16289	T16293	arg1Of	performed,(
R12515	T16289	T16299	arg1Of	performed,;
R12516	T16292	T16290	arg2Of	software,with
R12517	T16292	T16291	arg1Of	software,FlowJo
R12518	T16295	T16293	arg2Of	Star,(
R12519	T16295	T16294	arg1Of	Star,Tree
R1252	T1524	T1525	arg1Of	perforin,and
R12520	T16295	T16296	arg1Of	Star,","
R12521	T16297	T16296	arg2Of	Inc.,","
R12522	T16298	T16293	arg3Of	),(
R12523	T16302	T16300	arg1Of	cells,EL4
R12524	T16302	T16301	arg1Of	cells,target
R12525	T16302	T16303	arg1Of	cells,(
R12526	T16302	T16307	arg1Of	cells,were
R12527	T16302	T16308	arg2Of	cells,gated
R12528	T16305	T16303	arg2Of	events,(
R12529	T16305	T16304	arg1Of	events,CD8-negative
R1253	T1525	T1523	arg2Of	and,containing
R12530	T16306	T16303	arg3Of	),(
R12531	T16308	T16307	arg2Of	gated,were
R12532	T16308	T16310	arg1Of	gated,and
R12533	T16310	T16299	arg2Of	and,;
R12534	T16310	T16309	arg1Of	and,","
R12535	T16312	T16311	arg1Of	percentage,the
R12536	T16312	T16313	arg1Of	percentage,of
R12537	T16312	T16318	arg1Of	percentage,was
R12538	T16312	T16319	arg2Of	percentage,determined
R12539	T16317	T16313	arg2Of	cells,of
R1254	T1526	T1525	arg2Of	granzymes,and
R12540	T16317	T16314	arg1Of	cells,Annexin
R12541	T16317	T16315	arg1Of	cells,V+
R12542	T16317	T16316	arg1Of	cells,target
R12543	T16319	T16310	arg2Of	determined,and
R12544	T16319	T16318	arg2Of	determined,was
R1255	T1528	T1527	arg1Of	and,","
R1256	T1530	T1528	arg2Of	produce,and
R1257	T1530	T1529	arg1Of	produce,to
R1258	T1532	T1530	arg2Of	cytokines,produce
R1259	T1532	T1531	arg1Of	cytokines,inflammatory
R1260	T1532	T1534	arg1Of	cytokines,as
R1261	T1534	T1533	arg1Of	as,such
R1262	T1535	T1536	arg1Of	IFN-γ,and
R1263	T1536	T1534	arg2Of	and,as
R1264	T1536	T1538	arg1Of	and,upon
R1265	T1536	T1540	arg1Of	and,through
R1266	T1537	T1536	arg2Of	TNF,and
R1267	T1539	T1538	arg2Of	restimulation,upon
R12670	T16510	T16511	arg1Of	Cytokine,and
R12671	T16512	T16511	arg2Of	surface,and
R12672	T16514	T16510	arg1Of	staining,Cytokine
R12673	T16514	T16512	arg1Of	staining,surface
R12674	T16514	T16513	arg1Of	staining,marker
R12675	T16516	T16515	arg1Of	assess,To
R12676	T16518	T16516	arg2Of	production,assess
R12677	T16518	T16517	arg1Of	production,cytokine
R12678	T16520	T16516	arg1Of	cells,assess
R12679	T16520	T16521	arg1Of	cells,were
R1268	T1542	T1540	arg2Of	TCR,through
R12680	T16520	T16522	arg2Of	cells,restimulated
R12681	T16520	T16536	arg2Of	cells,indicated
R12682	T16522	T16521	arg2Of	restimulated,were
R12683	T16522	T16523	arg1Of	restimulated,with
R12684	T16522	T16535	arg1Of	restimulated,unless
R12685	T16522	T16543	arg1Of	restimulated,and
R12686	T16524	T16525	arg1Of	10,nM
R12687	T16528	T16527	arg1Of	1,+
R12688	T16530	T16523	arg2Of	ionomycin,with
R12689	T16530	T16524	arg1Of	ionomycin,10
R1269	T1542	T1541	arg1Of	TCR,the
R12690	T16530	T16526	arg1Of	ionomycin,PMA
R12691	T16530	T16528	arg1Of	ionomycin,1
R12692	T16530	T16529	arg1Of	ionomycin,μM
R12693	T16530	T16531	arg1Of	ionomycin,for
R12694	T16533	T16531	arg2Of	h,for
R12695	T16533	T16532	arg1Of	h,6
R12696	T16533	T16534	arg1Of	h,(
R12697	T16536	T16535	arg2Of	indicated,unless
R12698	T16536	T16537	arg1Of	indicated,otherwise
R12699	T16536	T16538	arg1Of	indicated,in
R1270	T1542	T1543	arg1Of	TCR,(
R12700	T16540	T16538	arg2Of	figures,in
R12701	T16540	T16539	arg1Of	figures,the
R12702	T16540	T16541	arg1Of	figures,)
R12703	T16543	T16515	modOf	and,To
R12704	T16543	T16519	arg1Of	and,","
R12705	T16543	T16542	arg1Of	and,","
R12706	T16546	T16544	arg1Of	stains,intracellular
R12707	T16546	T16545	arg1Of	stains,cytokine
R12708	T16546	T16547	arg1Of	stains,were
R12709	T16546	T16548	arg2Of	stains,performed
R1271	T1544	T1543	arg2Of	"1, 2",(
R12710	T16548	T16543	arg2Of	performed,and
R12711	T16548	T16547	arg2Of	performed,were
R12712	T16548	T16549	arg1Of	performed,as
R12713	T16551	T16549	arg2Of	described,as
R12714	T16551	T16550	arg1Of	described,previously
R12715	T16551	T16552	arg1Of	described,(
R12716	T16553	T16552	arg2Of	28,(
R12717	T16554	T16552	arg3Of	),(
R12718	T16556	T16555	arg1Of	detect,To
R12719	T16557	T16556	arg2Of	expression,detect
R1272	T1545	T1543	arg3Of	),(
R12720	T16557	T16558	arg1Of	expression,of
R12721	T16560	T16558	arg2Of	molecules,of
R12722	T16560	T16559	arg1Of	molecules,surface
R12723	T16562	T16556	arg1Of	cells,detect
R12724	T16562	T16563	arg1Of	cells,were
R12725	T16562	T16564	arg2Of	cells,washed
R12726	T16562	T16568	arg1Of	cells,resuspended
R12727	T16562	T16602	arg2Of	cells,incubated
R12728	T16562	T16613	arg1Of	cells,washed
R12729	T16562	T16616	arg1Of	cells,resuspended
R1273	T1547	T1546	arg1Of	vivo,In
R12730	T16564	T16563	arg2Of	washed,were
R12731	T16564	T16565	arg1Of	washed,in
R12732	T16564	T16567	arg1Of	washed,","
R12733	T16566	T16565	arg2Of	PBS,in
R12734	T16567	T16602	modOf	",",incubated
R12735	T16567	T16612	arg1Of	",",","
R12736	T16568	T16567	arg2Of	resuspended,","
R12737	T16568	T16569	arg1Of	resuspended,in
R12738	T16572	T16570	arg1Of	buffer,FACS
R12739	T16572	T16571	arg1Of	buffer,wash
R1274	T1548	T1547	arg1Of	experiments,vivo
R12740	T16572	T16573	arg1Of	buffer,(
R12741	T16572	T16590	arg1Of	buffer,containing
R12742	T16572	T16593	arg1Of	buffer,indicated
R12743	T16574	T16575	arg1Of	3,%
R12744	T16576	T16573	arg2Of	FBS,(
R12745	T16576	T16574	arg1Of	FBS,3
R12746	T16576	T16577	arg1Of	FBS,","
R12747	T16576	T16582	arg1Of	FBS,","
R12748	T16576	T16586	arg1Of	FBS,","
R12749	T16578	T16579	arg1Of	0.1,%
R1275	T1548	T1549	arg1Of	experiments,have
R12750	T16581	T16577	arg2Of	azide,","
R12751	T16581	T16578	arg1Of	azide,0.1
R12752	T16581	T16580	arg1Of	azide,sodium
R12753	T16584	T16583	arg1Of	mM,30
R12754	T16585	T16582	arg2Of	Hepes,","
R12755	T16585	T16584	arg1Of	Hepes,mM
R12756	T16588	T16586	arg2Of	PBS,","
R12757	T16588	T16587	arg1Of	PBS,1×
R12758	T16589	T16573	arg3Of	),(
R12759	T16592	T16590	arg2Of	antibodies,containing
R1276	T1548	T1550	arg1Of	experiments,elucidated
R12760	T16592	T16591	arg1Of	antibodies,the
R12761	T16593	T16568	arg2Of	indicated,resuspended
R12762	T16593	T16594	arg1Of	indicated,in
R12763	T16593	T16597	arg1Of	indicated,at
R12764	T16593	T16601	arg1Of	indicated,","
R12765	T16596	T16594	arg2Of	figures,in
R12766	T16596	T16595	arg1Of	figures,the
R12767	T16599	T16598	arg1Of	optimized,previously
R12768	T16600	T16597	arg2Of	concentrations,at
R12769	T16600	T16599	arg1Of	concentrations,optimized
R1277	T1550	T1549	arg2Of	elucidated,have
R12770	T16602	T16603	arg1Of	incubated,for
R12771	T16605	T16603	arg2Of	min,for
R12772	T16605	T16604	arg1Of	min,15
R12773	T16605	T16606	arg1Of	min,at
R12774	T16608	T16606	arg2Of	temperature,at
R12775	T16608	T16607	arg1Of	temperature,room
R12776	T16608	T16609	arg1Of	temperature,(
R12777	T16610	T16609	arg2Of	RT,(
R12778	T16611	T16609	arg3Of	),(
R12779	T16612	T16615	arg1Of	",",and
R1278	T1553	T1550	arg2Of	parameters,elucidated
R12780	T16613	T16612	arg2Of	washed,","
R12781	T16615	T16555	modOf	and,To
R12782	T16615	T16561	arg1Of	and,","
R12783	T16615	T16614	arg1Of	and,","
R12784	T16616	T16615	arg2Of	resuspended,and
R12785	T16616	T16617	arg1Of	resuspended,in
R12786	T16616	T16623	arg1Of	resuspended,before
R12787	T16616	T16625	arg1Of	resuspended,on
R12788	T16618	T16619	arg1Of	2,%
R12789	T16622	T16617	arg2Of	solution,in
R1279	T1553	T1551	arg1Of	parameters,many
R12790	T16622	T16618	arg1Of	solution,2
R12791	T16622	T16620	arg1Of	solution,formaldehyde
R12792	T16622	T16621	arg1Of	solution,fixative
R12793	T16624	T16623	arg2Of	acquisition,before
R12794	T16627	T16625	arg2Of	FACSCalibur,on
R12795	T16627	T16626	arg1Of	FACSCalibur,a
R12796	T16627	T16628	arg1Of	FACSCalibur,(
R12797	T16629	T16628	arg2Of	BD,(
R12798	T16630	T16628	arg3Of	),(
R1280	T1553	T1552	arg1Of	parameters,critical
R1281	T1553	T1554	arg1Of	parameters,governing
R1282	T1556	T1557	arg1Of	development,and
R1283	T1557	T1554	arg2Of	and,governing
R1284	T1557	T1555	arg1Of	and,the
R1285	T1557	T1559	arg1Of	and,of
R1286	T1558	T1557	arg2Of	evolution,and
R1287	T1562	T1559	arg2Of	responses,of
R1288	T1562	T1560	arg1Of	responses,primary
R1289	T1562	T1561	arg1Of	responses,CTL
R1290	T1562	T1563	arg1Of	responses,(
R1291	T1564	T1563	arg2Of	"3, 4",(
R1292	T1565	T1563	arg3Of	),(
R12928	T16786	T16785	arg1Of	transduction,Retroviral
R12929	T16786	T16787	arg1Of	transduction,of
R1293	T1568	T1566	arg2Of	study,In
R12930	T16791	T16787	arg2Of	cells,of
R12931	T16791	T16788	arg1Of	cells,primary
R12932	T16791	T16789	arg1Of	cells,CD8+
R12933	T16791	T16790	arg1Of	cells,T
R12934	T16794	T16793	arg1Of	experiments,transduction
R12935	T16794	T16792	arg2Of	experiments,For
R12936	T16797	T16796	arg1Of	supernatants,viral
R12937	T16797	T16798	arg1Of	supernatants,were
R12938	T16797	T16799	arg2Of	supernatants,generated
R12939	T16799	T16795	arg1Of	generated,","
R1294	T1568	T1567	arg1Of	study,this
R12940	T16799	T16792	arg1Of	generated,For
R12941	T16799	T16798	arg2Of	generated,were
R12942	T16799	T16800	arg1Of	generated,by
R12943	T16803	T16801	arg1Of	transfection,calcium
R12944	T16803	T16802	arg1Of	transfection,phosphate
R12945	T16803	T16804	arg1Of	transfection,of
R12946	T16803	T16807	arg1Of	transfection,and
R12947	T16806	T16804	arg2Of	cells,of
R12948	T16806	T16805	arg1Of	cells,Phoenix
R12949	T16807	T16800	arg2Of	and,by
R1295	T1570	T1571	arg1Of	we,have
R12950	T16808	T16807	arg2Of	concentration,and
R12951	T16808	T16809	arg1Of	concentration,by
R12952	T16808	T16812	arg1Of	concentration,at
R12953	T16811	T16809	arg2Of	centrifugation,by
R12954	T16811	T16810	arg1Of	centrifugation,overnight
R12955	T16814	T16812	arg2Of	g,at
R12956	T16814	T16813	arg1Of	g,"6,000"
R12957	T16817	T16815	arg2Of	h,At
R12958	T16817	T16816	arg1Of	h,∼42
R12959	T16817	T16818	arg1Of	h,after
R1296	T1570	T1572	arg1Of	we,used
R12960	T16822	T16818	arg2Of	activation,after
R12961	T16822	T16819	arg1Of	activation,the
R12962	T16822	T16820	arg1Of	activation,initial
R12963	T16822	T16821	arg1Of	activation,TCR
R12964	T16822	T16823	arg1Of	activation,of
R12965	T16822	T16828	arg1Of	activation,per
R12966	T16827	T16823	arg2Of	cells,of
R12967	T16827	T16824	arg1Of	cells,106
R12968	T16827	T16825	arg1Of	cells,CD8+
R12969	T16827	T16826	arg1Of	cells,T
R1297	T1570	T1586	arg1Of	we,define
R12970	T16829	T16828	arg2Of	well,per
R12971	T16829	T16830	arg1Of	well,in
R12972	T16832	T16830	arg2Of	plates,in
R12973	T16832	T16831	arg1Of	plates,12-well
R12974	T16836	T16834	arg1Of	media,the
R12975	T16836	T16835	arg1Of	media,culture
R12976	T16836	T16837	arg1Of	media,was
R12977	T16836	T16838	arg2Of	media,removed
R12978	T16836	T16840	arg2Of	media,replaced
R12979	T16838	T16839	arg1Of	removed,and
R1298	T1572	T1571	arg2Of	used,have
R12980	T16839	T16815	arg1Of	and,At
R12981	T16839	T16833	arg1Of	and,","
R12982	T16839	T16837	arg2Of	and,was
R12983	T16840	T16839	arg2Of	replaced,and
R12984	T16840	T16841	arg1Of	replaced,with
R12985	T16843	T16841	arg2Of	media,with
R12986	T16843	T16842	arg1Of	media,complete
R12987	T16843	T16844	arg2Of	media,supplemented
R12988	T16844	T16845	arg1Of	supplemented,with
R12989	T16848	T16845	arg2Of	polybrene,with
R1299	T1572	T1605	arg1Of	used,and
R12990	T16848	T16846	arg1Of	polybrene,8
R12991	T16848	T16847	arg1Of	polybrene,μg/ml
R12992	T16848	T16849	arg1Of	polybrene,containing
R12993	T16850	T16851	arg1Of	fresh,plus
R12994	T16852	T16851	arg2Of	concentrated,plus
R12995	T16853	T16849	arg2Of	virus,containing
R12996	T16853	T16850	arg1Of	virus,fresh
R12997	T16853	T16852	arg1Of	virus,concentrated
R12998	T16855	T16854	arg1Of	plates,The
R12999	T16855	T16856	arg1Of	plates,were
R1300	T1574	T1573	arg1Of	vitro,in
R13000	T16855	T16857	arg2Of	plates,centrifuged
R13001	T16855	T16867	arg1Of	plates,returning
R13002	T16857	T16856	arg2Of	centrifuged,were
R13003	T16857	T16858	arg1Of	centrifuged,at
R13004	T16857	T16861	arg1Of	centrifuged,for
R13005	T16857	T16864	arg1Of	centrifuged,at
R13006	T16857	T16866	arg1Of	centrifuged,before
R13007	T16860	T16858	arg2Of	g,at
R13008	T16860	T16859	arg1Of	g,700
R13009	T16863	T16861	arg2Of	h,for
R1301	T1575	T1572	arg2Of	systems,used
R13010	T16863	T16862	arg1Of	h,1
R13011	T16865	T16864	arg2Of	RT,at
R13012	T16867	T16866	arg2Of	returning,before
R13013	T16867	T16868	arg1Of	returning,to
R13014	T16867	T16870	arg1Of	returning,for
R13015	T16869	T16868	arg2Of	37°C,to
R13016	T16874	T16870	arg2Of	h,for
R13017	T16874	T16871	arg1Of	h,an
R13018	T16874	T16872	arg1Of	h,additional
R13019	T16874	T16873	arg1Of	h,5
R1302	T1575	T1574	arg1Of	systems,vitro
R13020	T16876	T16875	arg1Of	constructs,Retroviral
R13021	T16876	T16877	arg1Of	constructs,for
R13022	T16876	T16885	arg1Of	constructs,were
R13023	T16878	T16879	arg1Of	Eomes-VP16,and
R13024	T16879	T16877	arg2Of	and,for
R13025	T16884	T16879	arg2Of	vector,and
R13026	T16884	T16880	arg1Of	vector,the
R13027	T16884	T16881	arg1Of	vector,MIG
R13028	T16884	T16882	arg1Of	vector,control
R13029	T16884	T16883	arg1Of	vector,empty
R1303	T1575	T1577	arg1Of	systems,as
R13030	T16887	T16885	arg2Of	gift,were
R13031	T16887	T16886	arg1Of	gift,a
R13032	T16887	T16888	arg1Of	gift,from
R13033	T16890	T16888	arg2Of	Reiner,from
R13034	T16890	T16889	arg1Of	Reiner,S.L.
R13035	T16890	T16891	arg1Of	Reiner,(
R13036	T16890	T16900	arg1Of	Reiner,(
R13037	T16892	T16891	arg2Of	University,(
R13038	T16892	T16893	arg1Of	University,of
R13039	T16892	T16895	arg1Of	University,","
R1304	T1575	T1579	arg2Of	systems,developed
R13040	T16894	T16893	arg2Of	Pennsylvania,of
R13041	T16896	T16895	arg2Of	Philadelphia,","
R13042	T16896	T16897	arg1Of	Philadelphia,","
R13043	T16898	T16897	arg2Of	PA,","
R13044	T16899	T16891	arg3Of	),(
R13045	T16901	T16900	arg2Of	8,(
R13046	T16902	T16900	arg3Of	),(
R13047	T16967	T16963	arg1Of	analysis,ChIP
R13048	T16967	T16964	arg1Of	analysis,and
R13049	T16967	T16965	arg1Of	analysis,real-time
R1305	T1577	T1576	arg1Of	as,such
R13050	T16967	T16966	arg1Of	analysis,PCR
R13051	T16968	T16969	arg1Of	20,×
R13052	T16970	T16969	arg2Of	106,×
R13053	T16973	T16968	arg1Of	cells,20
R13054	T16973	T16970	arg1Of	cells,106
R13055	T16973	T16971	arg1Of	cells,CD8+
R13056	T16973	T16972	arg1Of	cells,T
R13057	T16973	T16974	arg1Of	cells,per
R13058	T16973	T16976	arg1Of	cells,were
R13059	T16973	T16977	arg2Of	cells,fixed
R1306	T1578	T1577	arg2Of	those,as
R13060	T16973	T17014	arg1Of	cells,were
R13061	T16973	T17015	arg2Of	cells,incubated
R13062	T16975	T16974	arg2Of	immunoprecipitation,per
R13063	T16977	T16976	arg2Of	fixed,were
R13064	T16977	T16978	arg1Of	fixed,by
R13065	T16977	T17013	arg1Of	fixed,and
R13066	T16979	T16978	arg2Of	adding,by
R13067	T16982	T16979	arg2Of	volume,adding
R13068	T16982	T16980	arg1Of	volume,a
R13069	T16982	T16981	arg1Of	volume,1/10th
R1307	T1579	T1580	modOf	developed,to
R13070	T16982	T16983	arg1Of	volume,of
R13071	T16982	T16986	arg1Of	volume,(
R13072	T16982	T17007	arg1Of	volume,to
R13073	T16985	T16983	arg2Of	solution,of
R13074	T16985	T16984	arg1Of	solution,fixation
R13075	T16987	T16988	arg1Of	11.1,%
R13076	T16989	T16987	arg1Of	formaldehyde,11.1
R13077	T16989	T16990	arg1Of	formaldehyde,","
R13078	T16990	T16994	arg1Of	",",","
R13079	T16991	T16992	arg1Of	100,mM
R1308	T1581	T1580	arg1Of	study,to
R13080	T16993	T16990	arg2Of	NaCl,","
R13081	T16993	T16991	arg1Of	NaCl,100
R13082	T16994	T16998	arg1Of	",",","
R13083	T16995	T16996	arg1Of	1,mM
R13084	T16997	T16994	arg2Of	EDTA,","
R13085	T16997	T16995	arg1Of	EDTA,1
R13086	T16998	T17002	arg1Of	",",","
R13087	T16999	T17000	arg1Of	0.5,mM
R13088	T17001	T16998	arg2Of	EGTA,","
R13089	T17001	T16999	arg1Of	EGTA,0.5
R1309	T1584	T1581	arg2Of	differentiation,study
R13090	T17002	T16986	arg2Of	",",(
R13091	T17003	T17004	arg1Of	50,mM
R13092	T17005	T17002	arg2Of	Hepes,","
R13093	T17005	T17003	arg1Of	Hepes,50
R13094	T17006	T16986	arg3Of	),(
R13095	T17009	T17007	arg2Of	volume,to
R13096	T17009	T17008	arg1Of	volume,1
R13097	T17009	T17010	arg1Of	volume,of
R13098	T17012	T17010	arg2Of	media,of
R13099	T17012	T17011	arg1Of	media,culture
R1310	T1584	T1582	arg1Of	differentiation,CD4+ T
R13100	T17015	T17013	arg2Of	incubated,and
R13101	T17015	T17014	arg2Of	incubated,were
R13102	T17015	T17016	arg1Of	incubated,for
R13103	T17017	T17018	arg1Of	10,or
R13104	T17019	T17018	arg2Of	30,or
R13105	T17020	T17016	arg2Of	min,for
R13106	T17020	T17017	arg1Of	min,10
R13107	T17020	T17019	arg1Of	min,30
R13108	T17020	T17021	arg1Of	min,at
R13109	T17022	T17021	arg2Of	RT,at
R1311	T1584	T1583	arg1Of	differentiation,cell
R13110	T17023	T17024	arg1Of	Fixation,was
R13111	T17023	T17025	arg2Of	Fixation,stopped
R13112	T17025	T17024	arg2Of	stopped,was
R13113	T17025	T17026	arg1Of	stopped,with
R13114	T17025	T17032	arg1Of	stopped,for
R13115	T17027	T17028	arg1Of	120,mM
R13116	T17029	T17026	arg2Of	glycine,with
R13117	T17029	T17027	arg1Of	glycine,120
R13118	T17029	T17030	arg1Of	glycine,on
R13119	T17031	T17030	arg2Of	ice,on
R1312	T1586	T1572	arg3Of	define,used
R13120	T17034	T17032	arg2Of	min,for
R13121	T17034	T17033	arg1Of	min,5
R13122	T17036	T17035	arg2Of	cells,Fixed
R13123	T17036	T17037	arg1Of	cells,were
R13124	T17036	T17038	arg2Of	cells,washed
R13125	T17038	T17037	arg2Of	washed,were
R13126	T17039	T17040	arg1Of	2×,with
R13127	T17039	T17072	arg1Of	2×,and
R13128	T17042	T17040	arg2Of	PBS,with
R13129	T17042	T17041	arg1Of	PBS,cold
R1313	T1586	T1585	arg1Of	define,to
R13130	T17042	T17043	arg1Of	PBS,","
R13131	T17044	T17043	arg2Of	1×,","
R13132	T17044	T17045	arg1Of	1×,with
R13133	T17047	T17045	arg2Of	solution,with
R13134	T17047	T17046	arg1Of	solution,cold
R13135	T17047	T17048	arg1Of	solution,I
R13136	T17047	T17049	arg1Of	solution,(
R13137	T17050	T17051	arg1Of	10,mM
R13138	T17052	T17050	arg1Of	Tris,10
R13139	T17052	T17053	arg1Of	Tris,[
R1314	T1589	T1586	arg2Of	basis,define
R13140	T17052	T17057	arg1Of	Tris,","
R13141	T17054	T17053	arg2Of	pH,[
R13142	T17054	T17055	arg1Of	pH,7.5
R13143	T17056	T17053	arg3Of	],[
R13144	T17057	T17061	arg1Of	",",","
R13145	T17058	T17059	arg1Of	10,mM
R13146	T17060	T17057	arg2Of	EDTA,","
R13147	T17060	T17058	arg1Of	EDTA,10
R13148	T17061	T17065	arg1Of	",",","
R13149	T17062	T17063	arg1Of	0.5,mM
R1315	T1589	T1587	arg1Of	basis,the
R13150	T17064	T17061	arg2Of	EGTA,","
R13151	T17064	T17062	arg1Of	EGTA,0.5
R13152	T17065	T17049	arg2Of	",",(
R13153	T17066	T17067	arg1Of	1,%
R13154	T17069	T17065	arg2Of	X-100,","
R13155	T17069	T17066	arg1Of	X-100,1
R13156	T17069	T17068	arg1Of	X-100,Triton
R13157	T17070	T17049	arg3Of	),(
R13158	T17072	T17038	arg3Of	and,washed
R13159	T17072	T17071	arg1Of	and,","
R1316	T1589	T1588	arg1Of	basis,molecular
R13160	T17073	T17072	arg2Of	1×,and
R13161	T17073	T17074	arg1Of	1×,with
R13162	T17076	T17074	arg2Of	solution,with
R13163	T17076	T17075	arg1Of	solution,cold
R13164	T17076	T17077	arg1Of	solution,II
R13165	T17076	T17078	arg1Of	solution,(
R13166	T17079	T17080	arg1Of	10,mM
R13167	T17081	T17079	arg1Of	Tris,10
R13168	T17081	T17082	arg1Of	Tris,[
R13169	T17081	T17086	arg1Of	Tris,","
R1317	T1589	T1590	arg1Of	basis,of
R13170	T17083	T17082	arg2Of	pH,[
R13171	T17083	T17084	arg1Of	pH,7.5
R13172	T17085	T17082	arg3Of	],[
R13173	T17086	T17090	arg1Of	",",","
R13174	T17087	T17088	arg1Of	1,mM
R13175	T17089	T17086	arg2Of	EDTA,","
R13176	T17089	T17087	arg1Of	EDTA,1
R13177	T17090	T17094	arg1Of	",",","
R13178	T17091	T17092	arg1Of	0.5,mM
R13179	T17093	T17090	arg2Of	EGTA,","
R1318	T1593	T1591	arg1Of	differentiation,effector
R13180	T17093	T17091	arg1Of	EGTA,0.5
R13181	T17094	T17078	arg2Of	",",(
R13182	T17095	T17096	arg1Of	200,mM
R13183	T17097	T17094	arg2Of	NaCl,","
R13184	T17097	T17095	arg1Of	NaCl,200
R13185	T17098	T17078	arg3Of	),(
R13186	T17100	T17099	arg2Of	washes,After
R13187	T17103	T17102	arg1Of	pellets,cell
R13188	T17103	T17104	arg1Of	pellets,were
R13189	T17103	T17105	arg2Of	pellets,resuspended
R1319	T1593	T1592	arg1Of	differentiation,CTL
R13190	T17105	T17099	arg1Of	resuspended,After
R13191	T17105	T17101	arg1Of	resuspended,","
R13192	T17105	T17104	arg2Of	resuspended,were
R13193	T17105	T17106	arg1Of	resuspended,at
R13194	T17105	T17111	arg1Of	resuspended,in
R13195	T17105	T17147	arg1Of	resuspended,and
R13196	T17107	T17108	arg1Of	40,×
R13197	T17109	T17108	arg2Of	106,×
R13198	T17110	T17106	arg2Of	cells/ml,at
R13199	T17110	T17107	arg1Of	cells/ml,40
R1320	T1593	T1594	arg1Of	differentiation,(
R13200	T17110	T17109	arg1Of	cells/ml,106
R13201	T17114	T17111	arg2Of	buffer,in
R13202	T17114	T17112	arg1Of	buffer,ChIP
R13203	T17114	T17113	arg1Of	buffer,lysis
R13204	T17114	T17115	arg1Of	buffer,(
R13205	T17118	T17116	arg1Of	NaCl,150
R13206	T17118	T17117	arg1Of	NaCl,mM
R13207	T17118	T17119	arg1Of	NaCl,","
R13208	T17119	T17127	arg1Of	",",","
R13209	T17120	T17121	arg1Of	25,mM
R1321	T1595	T1594	arg2Of	"5, 6",(
R13210	T17122	T17119	arg2Of	Tris,","
R13211	T17122	T17120	arg1Of	Tris,25
R13212	T17122	T17123	arg1Of	Tris,[
R13213	T17124	T17123	arg2Of	pH,[
R13214	T17124	T17125	arg1Of	pH,7.5
R13215	T17126	T17123	arg3Of	],[
R13216	T17127	T17132	arg1Of	",",","
R13217	T17128	T17129	arg1Of	1,%
R13218	T17131	T17127	arg2Of	X-100,","
R13219	T17131	T17128	arg1Of	X-100,1
R1322	T1596	T1594	arg3Of	),(
R13220	T17131	T17130	arg1Of	X-100,Triton
R13221	T17132	T17136	arg1Of	",",","
R13222	T17133	T17134	arg1Of	0.1,%
R13223	T17135	T17132	arg2Of	SDS,","
R13224	T17135	T17133	arg1Of	SDS,0.1
R13225	T17136	T17142	arg1Of	",",and
R13226	T17137	T17138	arg1Of	0.5,%
R13227	T17139	T17140	arg1Of	deoxycholate,plus
R13228	T17140	T17136	arg2Of	plus,","
R13229	T17140	T17137	arg1Of	plus,0.5
R1323	T1601	T1590	arg2Of	Eomesodermin,of
R13230	T17141	T17140	arg2Of	protease,plus
R13231	T17142	T17115	arg2Of	and,(
R13232	T17144	T17142	arg2Of	inhibitors,and
R13233	T17144	T17143	arg1Of	inhibitors,phosphatase
R13234	T17145	T17115	arg3Of	),(
R13235	T17147	T17146	arg1Of	and,","
R13236	T17148	T17149	arg1Of	chromatin,was
R13237	T17148	T17150	arg2Of	chromatin,sheared
R13238	T17150	T17147	arg2Of	sheared,and
R13239	T17150	T17149	arg2Of	sheared,was
R1324	T1604	T1602	arg3Of	),(
R13240	T17150	T17151	arg1Of	sheared,with
R13241	T17153	T17151	arg2Of	sonicator,with
R13242	T17153	T17152	arg1Of	sonicator,a
R13243	T17153	T17154	modOf	sonicator,to
R13244	T17155	T17154	arg1Of	yield,to
R13245	T17158	T17155	arg2Of	fragments,yield
R13246	T17158	T17156	arg1Of	fragments,0.5–1-kb
R13247	T17158	T17157	arg1Of	fragments,DNA
R13248	T17160	T17168	arg1Of	preclearing,and
R13249	T17163	T17160	arg2Of	chromatin,preclearing
R1325	T1601	T1593	arg1Of	Eomesodermin,differentiation
R13250	T17163	T17161	arg1Of	chromatin,the
R13251	T17163	T17162	arg1Of	chromatin,sheared
R13252	T17163	T17164	arg1Of	chromatin,with
R13253	T17167	T17164	arg2Of	beads,with
R13254	T17167	T17165	arg1Of	beads,protein
R13255	T17167	T17166	arg1Of	beads,A–sepharose
R13256	T17168	T17159	arg2Of	and,After
R13257	T17169	T17168	arg2Of	removing,and
R13258	T17169	T17172	arg1Of	removing,as
R13259	T17171	T17169	arg2Of	%,removing
R1326	T1601	T1597	arg1Of	Eomesodermin,". The"
R13260	T17171	T17170	arg1Of	%,5
R13261	T17174	T17172	arg2Of	chromatin,as
R13262	T17174	T17173	arg1Of	chromatin,input
R13263	T17176	T17160	arg1Of	immunoprecipitation,preclearing
R13264	T17176	T17169	arg1Of	immunoprecipitation,removing
R13265	T17176	T17177	arg1Of	immunoprecipitation,was
R13266	T17176	T17178	arg2Of	immunoprecipitation,performed
R13267	T17178	T17159	arg1Of	performed,After
R13268	T17178	T17175	arg1Of	performed,","
R13269	T17178	T17177	arg2Of	performed,was
R1327	T1601	T1598	arg1Of	Eomesodermin,T-box
R13270	T17178	T17179	arg1Of	performed,by
R13271	T17180	T17179	arg2Of	adding,by
R13272	T17183	T17180	arg2Of	amounts,adding
R13273	T17183	T17181	arg2Of	amounts,optimized
R13274	T17183	T17182	arg1Of	amounts,antibody
R13275	T17183	T17184	arg1Of	amounts,(
R13276	T17185	T17184	arg2Of	per,(
R13277	T17190	T17185	arg2Of	equivalents,per
R13278	T17190	T17186	arg1Of	equivalents,20
R13279	T17190	T17187	arg1Of	equivalents,×
R1328	T1605	T1566	arg1Of	and,In
R13280	T17190	T17188	arg1Of	equivalents,106
R13281	T17190	T17189	arg1Of	equivalents,cell
R13282	T17191	T17184	arg3Of	:,(
R13283	T17194	T17192	arg1Of	anti-Eomes,2.5
R13284	T17194	T17193	arg1Of	anti-Eomes,μg
R13285	T17194	T17195	arg1Of	anti-Eomes,","
R13286	T17195	T17202	arg1Of	",",","
R13287	T17195	T17203	arg2Of	",",followed
R13288	T17198	T17197	arg1Of	100,:
R13289	T17200	T17195	arg2Of	anti-Runx3,","
R1329	T1605	T1569	arg1Of	and,","
R13290	T17200	T17196	arg1Of	anti-Runx3,1
R13291	T17200	T17198	arg1Of	anti-Runx3,100
R13292	T17200	T17199	arg1Of	anti-Runx3,dilution
R13293	T17200	T17201	arg1Of	anti-Runx3,)
R13294	T17203	T17209	arg1Of	followed,;
R13295	T17206	T17203	arg1Of	incubation,followed
R13296	T17206	T17204	arg2Of	incubation,by
R13297	T17206	T17205	arg1Of	incubation,overnight
R13298	T17206	T17207	arg1Of	incubation,at
R13299	T17208	T17207	arg2Of	4°C,at
R1330	T1601	T1599	arg1Of	Eomesodermin,transcription
R13300	T17212	T17210	arg1Of	beads,protein
R13301	T17212	T17211	arg1Of	beads,A–sepharose
R13302	T17212	T17213	arg1Of	beads,were
R13303	T17212	T17214	arg2Of	beads,added
R13304	T17214	T17209	arg2Of	added,;
R13305	T17214	T17213	arg2Of	added,were
R13306	T17214	T17215	arg1Of	added,for
R13307	T17219	T17215	arg2Of	h,for
R13308	T17219	T17216	arg1Of	h,the
R13309	T17219	T17217	arg1Of	h,last
R1331	T1601	T1600	arg1Of	Eomesodermin,factors
R13310	T17219	T17218	arg1Of	h,3
R13311	T17219	T17220	arg1Of	h,of
R13312	T17223	T17220	arg2Of	period,of
R13313	T17223	T17221	arg1Of	period,the
R13314	T17223	T17222	arg1Of	period,incubation
R13315	T17224	T17225	arg1Of	Beads,were
R13316	T17224	T17226	arg2Of	Beads,washed
R13317	T17226	T17225	arg2Of	washed,were
R13318	T17227	T17228	arg1Of	2×,with
R13319	T17227	T17260	arg1Of	2×,","
R1332	T1606	T1607	arg1Of	T-bet,are
R13320	T17230	T17228	arg2Of	buffer,with
R13321	T17230	T17229	arg1Of	buffer,RIPA
R13322	T17230	T17231	arg1Of	buffer,(
R13323	T17232	T17233	arg1Of	50,mM
R13324	T17234	T17232	arg1Of	Tris,50
R13325	T17234	T17235	arg1Of	Tris,[
R13326	T17234	T17239	arg1Of	Tris,","
R13327	T17236	T17235	arg2Of	pH,[
R13328	T17236	T17237	arg1Of	pH,8
R13329	T17238	T17235	arg3Of	],[
R1333	T1606	T1608	arg2Of	T-bet,needed
R13330	T17239	T17243	arg1Of	",",","
R13331	T17240	T17241	arg1Of	150,mM
R13332	T17242	T17239	arg2Of	NaCl,","
R13333	T17242	T17240	arg1Of	NaCl,150
R13334	T17243	T17247	arg1Of	",",","
R13335	T17244	T17245	arg1Of	1,mM
R13336	T17246	T17243	arg2Of	EDTA,","
R13337	T17246	T17244	arg1Of	EDTA,1
R13338	T17247	T17231	arg2Of	",",(
R13339	T17247	T17251	arg1Of	",",","
R1334	T1601	T1602	arg1Of	Eomesodermin,(
R13340	T17248	T17249	arg1Of	1,%
R13341	T17250	T17247	arg2Of	NP-40,","
R13342	T17250	T17248	arg1Of	NP-40,1
R13343	T17252	T17253	arg1Of	0.1,%
R13344	T17254	T17251	arg2Of	SDS,","
R13345	T17254	T17252	arg1Of	SDS,0.1
R13346	T17254	T17255	arg1Of	SDS,","
R13347	T17256	T17257	arg1Of	0.5,%
R13348	T17258	T17255	arg2Of	deoxycholate,","
R13349	T17258	T17256	arg1Of	deoxycholate,0.5
R1335	T1603	T1602	arg2Of	Eomes,(
R13350	T17259	T17231	arg3Of	),(
R13351	T17260	T17292	arg1Of	",",and
R13352	T17261	T17260	arg2Of	1×,","
R13353	T17261	T17262	arg1Of	1×,with
R13354	T17265	T17262	arg2Of	buffer,with
R13355	T17265	T17263	arg1Of	buffer,high
R13356	T17265	T17264	arg1Of	buffer,salt
R13357	T17265	T17266	arg1Of	buffer,(
R13358	T17267	T17268	arg1Of	50,mM
R13359	T17269	T17267	arg1Of	Tris,50
R1336	T1608	T1605	arg2Of	needed,and
R13360	T17269	T17270	arg1Of	Tris,[
R13361	T17269	T17274	arg1Of	Tris,","
R13362	T17271	T17270	arg2Of	pH,[
R13363	T17271	T17272	arg1Of	pH,8
R13364	T17273	T17270	arg3Of	],[
R13365	T17274	T17278	arg1Of	",",","
R13366	T17275	T17276	arg1Of	500,mM
R13367	T17277	T17274	arg2Of	NaCl,","
R13368	T17277	T17275	arg1Of	NaCl,500
R13369	T17278	T17282	arg1Of	",",","
R1337	T1608	T1607	arg2Of	needed,are
R13370	T17279	T17280	arg1Of	1,mM
R13371	T17281	T17278	arg2Of	EDTA,","
R13372	T17281	T17279	arg1Of	EDTA,1
R13373	T17282	T17266	arg2Of	",",(
R13374	T17282	T17286	arg1Of	",",","
R13375	T17283	T17284	arg1Of	1,%
R13376	T17285	T17282	arg2Of	NP-40,","
R13377	T17285	T17283	arg1Of	NP-40,1
R13378	T17287	T17288	arg1Of	0.1,%
R13379	T17289	T17286	arg2Of	SDS,","
R1338	T1608	T1609	arg1Of	needed,for
R13380	T17289	T17287	arg1Of	SDS,0.1
R13381	T17290	T17266	arg3Of	),(
R13382	T17292	T17226	arg3Of	and,washed
R13383	T17292	T17291	arg1Of	and,","
R13384	T17293	T17292	arg2Of	1×,and
R13385	T17293	T17294	arg1Of	1×,with
R13386	T17296	T17294	arg2Of	buffer,with
R13387	T17296	T17295	arg1Of	buffer,TE
R13388	T17300	T17297	arg2Of	wash,After
R13389	T17300	T17298	arg1Of	wash,the
R1339	T1611	T1610	arg1Of	aspects,important
R13390	T17300	T17299	arg1Of	wash,last
R13391	T17302	T17303	arg1Of	DNA,was
R13392	T17302	T17304	arg2Of	DNA,eluted
R13393	T17304	T17297	arg1Of	eluted,After
R13394	T17304	T17301	arg1Of	eluted,","
R13395	T17304	T17303	arg2Of	eluted,was
R13396	T17304	T17305	arg1Of	eluted,by
R13397	T17306	T17305	arg2Of	resuspending,by
R13398	T17308	T17306	arg2Of	beads,resuspending
R13399	T17308	T17307	arg1Of	beads,the
R1340	T1611	T1612	arg1Of	aspects,of
R13400	T17308	T17309	arg1Of	beads,in
R13401	T17311	T17309	arg2Of	buffer,in
R13402	T17311	T17310	arg1Of	buffer,elution
R13403	T17311	T17312	arg1Of	buffer,(
R13404	T17313	T17314	arg1Of	1,%
R13405	T17315	T17312	arg2Of	SDS,(
R13406	T17315	T17313	arg1Of	SDS,1
R13407	T17315	T17316	arg1Of	SDS,","
R13408	T17317	T17318	arg1Of	100,mM
R13409	T17319	T17316	arg2Of	NaHCO3,","
R1341	T1611	T1614	arg1Of	aspects,and
R13410	T17319	T17317	arg1Of	NaHCO3,100
R13411	T17320	T17312	arg3Of	),(
R13412	T17322	T17323	arg1Of	input,and
R13413	T17323	T17321	arg1Of	and,Both
R13414	T17323	T17326	arg1Of	and,were
R13415	T17323	T17328	arg2Of	and,treated
R13416	T17325	T17323	arg2Of	chromatin,and
R13417	T17325	T17324	arg1Of	chromatin,ChIP
R13418	T17328	T17326	arg2Of	treated,were
R13419	T17328	T17327	arg1Of	treated,then
R1342	T1613	T1612	arg2Of	effector,of
R13420	T17328	T17329	arg1Of	treated,with
R13421	T17328	T17337	arg1Of	treated,for
R13422	T17328	T17342	arg1Of	treated,","
R13423	T17328	T17343	modOf	treated,followed
R13424	T17331	T17329	arg2Of	A,with
R13425	T17331	T17330	arg1Of	A,RNase
R13426	T17331	T17332	arg1Of	A,(
R13427	T17335	T17332	arg2Of	total,(
R13428	T17335	T17333	arg1Of	total,5
R13429	T17335	T17334	arg1Of	total,μg
R1343	T1705	T1686	arg1Of	resolved,Nevertheless
R13430	T17336	T17332	arg3Of	),(
R13431	T17339	T17337	arg2Of	h,for
R13432	T17339	T17338	arg1Of	h,1
R13433	T17339	T17340	arg1Of	h,at
R13434	T17341	T17340	arg2Of	37°C,at
R13435	T17346	T17343	arg1Of	addition,followed
R13436	T17346	T17344	arg2Of	addition,by
R13437	T17346	T17345	arg1Of	addition,the
R13438	T17346	T17347	arg1Of	addition,of
R13439	T17349	T17348	arg1Of	K,proteinase
R1344	T1614	T1609	arg2Of	and,for
R13440	T17349	T17350	arg1Of	K,(
R13441	T17349	T17355	arg1Of	K,and
R13442	T17353	T17350	arg2Of	total,(
R13443	T17353	T17351	arg1Of	total,100
R13444	T17353	T17352	arg1Of	total,μg
R13445	T17354	T17350	arg3Of	),(
R13446	T17355	T17347	arg2Of	and,of
R13447	T17357	T17355	arg2Of	incubation,and
R13448	T17357	T17356	arg1Of	incubation,overnight
R13449	T17357	T17358	arg1Of	incubation,at
R1345	T1617	T1614	arg2Of	differentiation,and
R13450	T17357	T17360	modOf	incubation,to
R13451	T17359	T17358	arg2Of	65°C,at
R13452	T17361	T17360	arg1Of	reverse,to
R13453	T17362	T17361	arg2Of	cross-linking,reverse
R13454	T17363	T17364	arg1Of	DNA,was
R13455	T17363	T17366	arg2Of	DNA,purified
R13456	T17363	T17384	arg1Of	DNA,resuspended
R13457	T17366	T17367	arg1Of	purified,with
R13458	T17366	T17377	arg1Of	purified,according
R13459	T17366	T17383	arg1Of	purified,and
R1346	T1617	T1615	arg1Of	differentiation,memory
R13460	T17369	T17367	arg2Of	columns,with
R13461	T17369	T17368	arg1Of	columns,QIAquick
R13462	T17369	T17370	arg1Of	columns,(
R13463	T17373	T17370	arg2Of	Kit,(
R13464	T17373	T17371	arg1Of	Kit,Gel
R13465	T17373	T17372	arg1Of	Kit,Extraction
R13466	T17373	T17374	arg1Of	Kit,;
R13467	T17375	T17374	arg2Of	QIAGEN,;
R13468	T17376	T17370	arg3Of	),(
R13469	T17378	T17377	arg2Of	to,according
R1347	T1617	T1616	arg1Of	differentiation,CTL
R13470	T17380	T17379	arg1Of	manufacturer,the
R13471	T17380	T17381	arg2Of	manufacturer,'s
R13472	T17382	T17378	arg2Of	instructions,to
R13473	T17382	T17381	arg1Of	instructions,'s
R13474	T17383	T17364	arg2Of	and,was
R13475	T17383	T17365	arg1Of	and,then
R13476	T17384	T17383	arg2Of	resuspended,and
R13477	T17384	T17385	arg1Of	resuspended,in
R13478	T17388	T17385	arg2Of	volume,in
R13479	T17388	T17386	arg1Of	volume,a
R1348	T1617	T1618	arg1Of	differentiation,(
R13480	T17388	T17387	arg1Of	volume,50-μl
R13481	T17392	T17389	arg2Of	detection,For
R13482	T17392	T17390	arg1Of	detection,real-time
R13483	T17392	T17391	arg1Of	detection,PCR
R13484	T17392	T17393	arg1Of	detection,of
R13485	T17395	T17393	arg2Of	targets,of
R13486	T17395	T17394	arg1Of	targets,immunoprecipitated
R13487	T17395	T17396	arg1Of	targets,using
R13488	T17399	T17398	arg1Of	Green,SYBR
R13489	T17401	T17396	arg2Of	Kit,using
R1349	T1619	T1618	arg2Of	7,(
R13490	T17401	T17397	arg1Of	Kit,the
R13491	T17401	T17399	arg1Of	Kit,Green
R13492	T17401	T17400	arg1Of	Kit,PCR
R13493	T17405	T17403	arg1Of	curve,a
R13494	T17405	T17404	arg1Of	curve,standard
R13495	T17405	T17406	arg1Of	curve,was
R13496	T17405	T17407	arg2Of	curve,obtained
R13497	T17407	T17406	arg2Of	obtained,was
R13498	T17407	T17408	arg1Of	obtained,with
R13499	T17407	T17418	arg1Of	obtained,and
R1350	T1620	T1618	arg3Of	),(
R13500	T17410	T17408	arg2Of	dilutions,with
R13501	T17410	T17409	arg1Of	dilutions,serial
R13502	T17410	T17411	arg1Of	dilutions,of
R13503	T17413	T17411	arg2Of	DNA,of
R13504	T17413	T17412	arg1Of	DNA,input
R13505	T17413	T17414	arg1Of	DNA,for
R13506	T17416	T17414	arg2Of	sample,for
R13507	T17416	T17415	arg1Of	sample,each
R13508	T17418	T17389	arg1Of	and,For
R13509	T17418	T17402	arg1Of	and,","
R1351	T1623	T1621	arg2Of	mice,In
R13510	T17418	T17417	arg1Of	and,","
R13511	T17422	T17419	arg1Of	DNA,1
R13512	T17422	T17420	arg1Of	DNA,μl
R13513	T17422	T17421	arg1Of	DNA,ChIP
R13514	T17422	T17423	arg1Of	DNA,was
R13515	T17422	T17424	arg2Of	DNA,used
R13516	T17424	T17418	arg2Of	used,and
R13517	T17424	T17423	arg2Of	used,was
R13518	T17424	T17425	arg1Of	used,per
R13519	T17427	T17425	arg2Of	reaction,per
R1352	T1623	T1622	arg1Of	mice,uninfected
R13520	T17427	T17426	arg1Of	reaction,PCR
R13521	T17427	T17428	arg1Of	reaction,(
R13522	T17429	T17428	arg2Of	performed,(
R13523	T17429	T17430	arg1Of	performed,in
R13524	T17431	T17430	arg2Of	duplicates,in
R13525	T17432	T17428	arg3Of	),(
R13526	T17434	T17433	arg1Of	curves,Melt
R13527	T17434	T17435	arg1Of	curves,and
R13528	T17435	T17438	arg1Of	and,were
R13529	T17435	T17439	arg2Of	and,analyzed
R1353	T1705	T1687	arg1Of	resolved,","
R13530	T17437	T17435	arg2Of	gels,and
R13531	T17437	T17436	arg1Of	gels,agarose
R13532	T17439	T17438	arg2Of	analyzed,were
R13533	T17439	T17440	modOf	analyzed,to
R13534	T17441	T17440	arg1Of	ensure,to
R13535	T17442	T17441	arg2Of	amplification,ensure
R13536	T17442	T17443	arg1Of	amplification,of
R13537	T17446	T17443	arg2Of	sequences,of
R13538	T17446	T17444	arg1Of	sequences,specific
R13539	T17446	T17445	arg1Of	sequences,target
R1354	T1705	T1701	arg2Of	resolved,have
R13540	T17447	T17458	arg1Of	Refer,for
R13541	T17450	T17447	arg2Of	S1,Refer
R13542	T17450	T17448	arg1Of	S1,to
R13543	T17450	T17449	arg1Of	S1,Table
R13544	T17450	T17451	arg1Of	S1,(
R13545	T17452	T17451	arg2Of	available,(
R13546	T17452	T17453	arg1Of	available,at
R13547	T17454	T17453	arg2Of	http,at
R13548	T17454	T17455	arg1Of	http,:
R13549	T17456	T17455	arg2Of	//www.jem.org/cgi/content/full/jem.20081242/DC1,:
R1355	T1626	T1625	arg1Of	deletion,compound
R13550	T17457	T17451	arg3Of	),(
R13551	T17460	T17458	arg2Of	list,for
R13552	T17460	T17459	arg1Of	list,a
R13553	T17460	T17461	arg1Of	list,of
R13554	T17463	T17461	arg2Of	sets,of
R13555	T17463	T17462	arg1Of	sets,primer
R13556	T17465	T17464	arg1Of	data,The
R13557	T17465	T17466	arg1Of	data,are
R13558	T17465	T17467	arg2Of	data,presented
R13559	T17467	T17466	arg2Of	presented,are
R1356	T1626	T1627	arg1Of	deletion,of
R13560	T17467	T17468	arg1Of	presented,as
R13561	T17467	T17479	arg1Of	presented,","
R13562	T17467	T17480	modOf	presented,assuming
R13563	T17470	T17468	arg2Of	number,as
R13564	T17470	T17469	arg1Of	number,the
R13565	T17470	T17471	arg1Of	number,of
R13566	T17474	T17471	arg2Of	sequences,of
R13567	T17474	T17472	arg2Of	sequences,immunoprecipitated
R13568	T17474	T17473	arg1Of	sequences,target
R13569	T17474	T17475	arg1Of	sequences,relative
R1357	T1626	T1634	arg1Of	deletion,is
R13570	T17475	T17476	arg1Of	relative,to
R13571	T17478	T17476	arg2Of	chromatin,to
R13572	T17478	T17477	arg1Of	chromatin,input
R13573	T17482	T17480	arg2Of	copies,assuming
R13574	T17482	T17481	arg1Of	copies,two
R13575	T17482	T17483	arg1Of	copies,of
R13576	T17482	T17486	arg1Of	copies,per
R13577	T17485	T17483	arg2Of	sequence,of
R13578	T17485	T17484	arg1Of	sequence,target
R13579	T17487	T17486	arg2Of	cell,per
R1358	T1626	T1635	arg2Of	deletion,associated
R13580	T17487	T17488	arg1Of	cell,equivalent
R13581	T17487	T17489	arg2Of	cell,used
R13582	T17489	T17490	arg1Of	used,for
R13583	T17492	T17490	arg2Of	ChIP,for
R13584	T17492	T17491	arg1Of	ChIP,the
R1359	T1705	T1702	arg1Of	resolved,not
R1360	T1705	T1703	arg1Of	resolved,yet
R1361	T1705	T1704	arg2Of	resolved,been
R1362	T1705	T1706	arg1Of	resolved,:
R1363	T1631	T1629	arg2Of	T-bet,(
R1364	T1631	T1630	arg1Of	T-bet,encoding
R1365	T1632	T1629	arg3Of	),(
R1366	T1708	T1707	arg2Of	particular,in
R1367	T1633	T1627	arg2Of	and eomesodermin genes,of
R1368	T1633	T1628	arg1Of	and eomesodermin genes,the Tbx21
R1369	T1633	T1629	arg1Of	and eomesodermin genes,(
R1370	T1710	T1711	arg1Of	it,is
R1371	T1635	T1621	arg1Of	associated,In
R1372	T1635	T1624	arg1Of	associated,","
R1373	T1635	T1634	arg2Of	associated,is
R1374	T1635	T1636	arg1Of	associated,with
R1375	T1639	T1636	arg2Of	loss,with
R1376	T1710	T1713	arg2Of	it,known
R1377	T1713	T1706	arg2Of	known,:
R1378	T1639	T1637	arg1Of	loss,a
R1379	T1639	T1638	arg1Of	loss,selective
R1380	T1639	T1640	arg1Of	loss,of
R1381	T1639	T1643	arg1Of	loss,with
R1382	T1642	T1640	arg2Of	cells,of
R1383	T1642	T1641	arg1Of	cells,CD8+ T
R1384	T1713	T1707	arg1Of	known,in
R13842	T17829	T17830	arg1Of	Northern,and
R13843	T17831	T17830	arg2Of	Western,and
R13844	T17833	T17829	arg1Of	analyses,Northern
R13845	T17833	T17831	arg1Of	analyses,Western
R13846	T17833	T17832	arg1Of	analyses,blot
R13847	T17835	T17834	arg1Of	isolation,RNA
R13848	T17835	T17844	arg2Of	isolation,described
R13849	T17839	T17836	arg1Of	analysis,and
R1385	T1713	T1709	arg1Of	known,","
R13850	T17839	T17837	arg1Of	analysis,Northern
R13851	T17839	T17838	arg1Of	analysis,blot
R13852	T17839	T17840	arg1Of	analysis,was
R13853	T17839	T17841	arg2Of	analysis,performed
R13854	T17841	T17840	arg2Of	performed,was
R13855	T17841	T17842	arg1Of	performed,as
R13856	T17842	T17843	arg1Of	as,previously
R13857	T17844	T17840	modOf	described,was
R13858	T17844	T17845	arg1Of	described,(
R13859	T17846	T17845	arg2Of	29,(
R1386	T1645	T1643	arg2Of	IL-2Rβ–high,with
R13860	T17847	T17845	arg3Of	),(
R13861	T17849	T17848	arg2Of	brief,In
R13862	T17852	T17851	arg1Of	μg,10
R13863	T17852	T17853	arg1Of	μg,of
R13864	T17852	T17856	arg1Of	μg,was
R13865	T17852	T17857	arg2Of	μg,loaded
R13866	T17852	T17861	arg2Of	μg,transferred
R13867	T17855	T17853	arg2Of	RNA,of
R13868	T17855	T17854	arg1Of	RNA,total
R13869	T17857	T17858	arg1Of	loaded,per
R1387	T1645	T1644	arg1Of	IL-2Rβ–high,an
R13870	T17857	T17860	arg1Of	loaded,and
R13871	T17859	T17858	arg2Of	lane,per
R13872	T17860	T17848	arg1Of	and,In
R13873	T17860	T17850	arg1Of	and,","
R13874	T17860	T17856	arg2Of	and,was
R13875	T17861	T17860	arg2Of	transferred,and
R13876	T17861	T17862	arg1Of	transferred,to
R13877	T17864	T17863	arg1Of	charged,positively
R13878	T17866	T17862	arg2Of	membranes,to
R13879	T17866	T17864	arg1Of	membranes,charged
R1388	T1645	T1646	arg1Of	IL-2Rβ–high,","
R13880	T17866	T17865	arg1Of	membranes,nylon
R13881	T17866	T17867	arg1Of	membranes,(
R13882	T17866	T17873	arg1Of	membranes,","
R13883	T17866	T17874	arg1Of	membranes,which
R13884	T17866	T17875	arg1Of	membranes,was
R13885	T17866	T17876	arg2Of	membranes,confirmed
R13886	T17868	T17869	arg1Of	Hybond-N+,;
R13887	T17869	T17867	arg2Of	;,(
R13888	T17871	T17869	arg2Of	Healthcare,;
R13889	T17871	T17870	arg1Of	Healthcare,GE
R1389	T1713	T1711	arg2Of	known,is
R13890	T17872	T17867	arg3Of	),(
R13891	T17876	T17875	arg2Of	confirmed,was
R13892	T17880	T17876	arg1Of	staining,confirmed
R13893	T17880	T17877	arg2Of	staining,by
R13894	T17880	T17878	arg1Of	staining,ethidium
R13895	T17880	T17879	arg1Of	staining,bromide
R13896	T17880	T17881	arg1Of	staining,of
R13897	T17880	T17885	arg1Of	staining,on
R13898	T17884	T17881	arg2Of	species,of
R13899	T17884	T17882	arg1Of	species,ribosomal
R1390	T1713	T1712	arg1Of	known,not
R13900	T17884	T17883	arg1Of	species,RNA
R13901	T17887	T17885	arg2Of	membrane,on
R13902	T17887	T17886	arg1Of	membrane,the
R13903	T17888	T17889	arg1Of	Membranes,were
R13904	T17888	T17890	arg2Of	Membranes,hybridized
R13905	T17890	T17889	arg2Of	hybridized,were
R13906	T17890	T17891	arg1Of	hybridized,with
R13907	T17892	T17893	arg1Of	1,ng/ml
R13908	T17894	T17895	arg1Of	α-,[
R13909	T17896	T17895	arg2Of	32P,[
R1391	T1717	T1715	arg1Of	factors,these
R13910	T17897	T17895	arg3Of	],[
R13911	T17898	T17894	arg1Of	dCTP–labeled,α-
R13912	T17902	T17891	arg2Of	probe,with
R13913	T17902	T17892	arg1Of	probe,1
R13914	T17902	T17898	arg1Of	probe,dCTP–labeled
R13915	T17902	T17899	arg1Of	probe,trichloroacetic
R13916	T17902	T17900	arg1Of	probe,acid
R13917	T17902	T17901	arg1Of	probe,precipitable
R13918	T17902	T17903	arg1Of	probe,in
R13919	T17906	T17903	arg2Of	buffer,in
R1392	T1648	T1646	arg2Of	phenotype,","
R13920	T17906	T17904	arg1Of	buffer,ExpressHyb
R13921	T17906	T17905	arg1Of	buffer,hybridization
R13922	T17906	T17907	arg1Of	buffer,(
R13923	T17909	T17907	arg2Of	Laboratories,(
R13924	T17909	T17908	arg1Of	Laboratories,Clontech
R13925	T17909	T17910	arg1Of	Laboratories,","
R13926	T17911	T17910	arg2Of	Inc.,","
R13927	T17912	T17907	arg3Of	),(
R13928	T17915	T17913	arg1Of	probes,All
R13929	T17915	T17914	arg1Of	probes,cDNA
R1393	T1648	T1647	arg1Of	phenotype,memory
R13930	T17915	T17916	arg1Of	probes,were
R13931	T17915	T17917	arg2Of	probes,confirmed
R13932	T17915	T17919	arg1Of	probes,have
R13933	T17917	T17916	arg2Of	confirmed,were
R13934	T17919	T17917	arg3Of	have,confirmed
R13935	T17919	T17918	arg1Of	have,to
R13936	T17919	T17924	arg1Of	have,under
R13937	T17923	T17919	arg2Of	specificity,have
R13938	T17923	T17920	arg1Of	specificity,the
R13939	T17923	T17921	arg1Of	specificity,appropriate
R1394	T1648	T1649	arg1Of	phenotype,(
R13940	T17923	T17922	arg1Of	specificity,single-copy
R13941	T17926	T17924	arg2Of	conditions,under
R13942	T17926	T17925	arg1Of	conditions,these
R13943	T17926	T17927	arg1Of	conditions,using
R13944	T17931	T17927	arg2Of	analysis,using
R13945	T17931	T17928	arg1Of	analysis,genomic
R13946	T17931	T17929	arg1Of	analysis,Southern
R13947	T17931	T17930	arg1Of	analysis,blot
R13948	T17933	T17932	arg1Of	intensities,Band
R13949	T17933	T17934	arg1Of	intensities,were
R1395	T1650	T1649	arg2Of	8,(
R13950	T17933	T17935	arg2Of	intensities,acquired
R13951	T17935	T17934	arg2Of	acquired,were
R13952	T17935	T17936	arg1Of	acquired,by
R13953	T17937	T17936	arg2Of	phosphorimaging,by
R13954	T17938	T17937	arg2Of	analysis,phosphorimaging
R13955	T17941	T17939	arg2Of	analysis,For
R13956	T17941	T17940	arg1Of	analysis,Western
R13957	T17945	T17943	arg1Of	lysates,whole-cell
R13958	T17945	T17944	arg1Of	lysates,protein
R13959	T17945	T17946	arg1Of	lysates,were
R1396	T1651	T1649	arg3Of	),(
R13960	T17945	T17947	arg2Of	lysates,obtained
R13961	T17945	T17996	arg1Of	lysates,resuspending
R13962	T17945	T18005	arg1Of	lysates,incubating
R13963	T17947	T17939	arg1Of	obtained,For
R13964	T17947	T17942	arg1Of	obtained,","
R13965	T17947	T17946	arg2Of	obtained,were
R13966	T17947	T17948	arg1Of	obtained,from
R13967	T17947	T17952	arg1Of	obtained,at
R13968	T17947	T17960	arg1Of	obtained,during
R13969	T17947	T17995	arg1Of	obtained,by
R1397	T1652	T1653	arg1Of	Mice,deficient
R13970	T17951	T17948	arg2Of	cells,from
R13971	T17951	T17949	arg1Of	cells,CD8+
R13972	T17951	T17950	arg1Of	cells,T
R13973	T17954	T17952	arg2Of	time,at
R13974	T17954	T17953	arg1Of	time,the
R13975	T17955	T17956	arg1Of	points,indicated
R13976	T17956	T17954	arg2Of	indicated,time
R13977	T17956	T17957	arg1Of	indicated,in
R13978	T17959	T17957	arg2Of	figures,in
R13979	T17959	T17958	arg1Of	figures,the
R1398	T1652	T1662	arg1Of	Mice,have
R13980	T17962	T17960	arg2Of	expansion,during
R13981	T17962	T17961	arg1Of	expansion,clonal
R13982	T17962	T17963	arg1Of	expansion,in
R13983	T17962	T17967	arg1Of	expansion,with
R13984	T17964	T17965	arg1Of	100,U/ml
R13985	T17966	T17963	arg2Of	IL-2,in
R13986	T17966	T17964	arg1Of	IL-2,100
R13987	T17969	T17967	arg2Of	buffer,with
R13988	T17969	T17968	arg1Of	buffer,lysis
R13989	T17969	T17970	arg1Of	buffer,(
R1399	T1652	T1663	arg1Of	Mice,impaired
R13990	T17971	T17972	arg1Of	50,mM
R13991	T17973	T17971	arg1Of	Tris,50
R13992	T17973	T17974	arg1Of	Tris,[
R13993	T17973	T17978	arg1Of	Tris,","
R13994	T17975	T17974	arg2Of	pH,[
R13995	T17975	T17976	arg1Of	pH,7.5
R13996	T17977	T17974	arg3Of	],[
R13997	T17978	T17970	arg2Of	",",(
R13998	T17979	T17980	arg1Of	150,mM
R13999	T17981	T17979	arg1Of	NaCl,150
R1400	T1652	T1669	arg1Of	Mice,manifest
R14000	T17981	T17982	arg1Of	NaCl,","
R14001	T17982	T17986	arg1Of	",",","
R14002	T17983	T17984	arg1Of	10,%
R14003	T17985	T17982	arg2Of	glycerol,","
R14004	T17985	T17983	arg1Of	glycerol,10
R14005	T17986	T17990	arg1Of	",",","
R14006	T17987	T17988	arg1Of	5,mM
R14007	T17989	T17986	arg2Of	EDTA,","
R14008	T17989	T17987	arg1Of	EDTA,5
R14009	T17990	T17978	arg2Of	",",","
R1401	T1652	T1675	arg1Of	Mice,fail
R14010	T17991	T17992	arg1Of	1,%
R14011	T17993	T17990	arg2Of	NP-40,","
R14012	T17993	T17991	arg1Of	NP-40,1
R14013	T17994	T17970	arg3Of	),(
R14014	T17996	T17998	arg1Of	resuspending,in
R14015	T17996	T18004	arg1Of	resuspending,and
R14016	T17997	T17996	arg2Of	samples,resuspending
R14017	T18000	T17998	arg2Of	μl,in
R14018	T18000	T17999	arg1Of	μl,10
R14019	T18000	T18001	arg1Of	μl,per
R1402	T1652	T1677	arg1Of	Mice,control
R14020	T18003	T18001	arg2Of	cells,per
R14021	T18003	T18002	arg1Of	cells,106
R14022	T18004	T17995	arg2Of	and,by
R14023	T18005	T18004	arg2Of	incubating,and
R14024	T18005	T18006	arg1Of	incubating,on
R14025	T18005	T18008	arg1Of	incubating,for
R14026	T18005	T18011	arg1Of	incubating,in
R14027	T18007	T18006	arg2Of	ice,on
R14028	T18010	T18008	arg2Of	min,for
R14029	T18010	T18009	arg1Of	min,30
R1403	T1653	T1654	arg1Of	deficient,for
R14030	T18013	T18011	arg2Of	presence,in
R14031	T18013	T18012	arg1Of	presence,the
R14032	T18013	T18014	arg1Of	presence,of
R14033	T18016	T18014	arg2Of	inhibitors,of
R14034	T18016	T18015	arg1Of	inhibitors,protease
R14035	T18018	T18017	arg1Of	analysis,Immunoblot
R14036	T18018	T18019	arg1Of	analysis,was
R14037	T18018	T18020	arg2Of	analysis,performed
R14038	T18020	T18019	arg2Of	performed,was
R14039	T18020	T18021	arg1Of	performed,with
R1404	T1656	T1657	arg1Of	T-bet,and
R14040	T18023	T18021	arg2Of	antibodies,with
R14041	T18023	T18022	arg1Of	antibodies,the
R14042	T18023	T18024	arg2Of	antibodies,indicated
R14043	T18024	T18025	arg1Of	indicated,in
R14044	T18024	T18028	arg1Of	indicated,after
R14045	T18027	T18025	arg2Of	figures,in
R14046	T18027	T18026	arg1Of	figures,the
R14047	T18029	T18028	arg2Of	SDS-PAGE,after
R14048	T18029	T18030	arg1Of	SDS-PAGE,(
R14049	T18032	T18031	arg1Of	μg,10–30
R1405	T1657	T1654	arg2Of	and,for
R14050	T18032	T18033	arg1Of	μg,of
R14051	T18032	T18036	arg1Of	μg,was
R14052	T18032	T18037	arg2Of	μg,loaded
R14053	T18035	T18033	arg2Of	protein,of
R14054	T18035	T18034	arg1Of	protein,total
R14055	T18037	T18030	arg2Of	loaded,(
R14056	T18037	T18036	arg2Of	loaded,was
R14057	T18037	T18038	arg1Of	loaded,per
R14058	T18039	T18038	arg2Of	well,per
R14059	T18040	T18030	arg3Of	),(
R1406	T1657	T1655	arg1Of	and,both
R14060	T18041	T18042	arg1Of	Quantification,of
R14061	T18041	T18045	arg1Of	Quantification,was
R14062	T18041	T18046	arg2Of	Quantification,performed
R14063	T18041	T18059	arg2Of	Quantification,normalized
R14064	T18041	T18061	arg1Of	Quantification,loading
R14065	T18044	T18042	arg2Of	protein,of
R14066	T18044	T18043	arg2Of	protein,detected
R14067	T18046	T18047	arg1Of	performed,with
R14068	T18046	T18058	arg1Of	performed,and
R14069	T18051	T18049	arg1Of	Box,Intelligent
R1407	T1657	T1659	arg1Of	and,in
R14070	T18051	T18050	arg1Of	Box,Dark
R14071	T18052	T18047	arg2Of	unit,with
R14072	T18052	T18048	arg1Of	unit,an
R14073	T18052	T18051	arg1Of	unit,Box
R14074	T18052	T18053	arg1Of	unit,(
R14075	T18054	T18053	arg2Of	LAS-3000,(
R14076	T18054	T18055	arg1Of	LAS-3000,;
R14077	T18056	T18055	arg2Of	Fujifilm,;
R14078	T18057	T18053	arg3Of	),(
R14079	T18058	T18045	arg2Of	and,was
R1408	T1658	T1657	arg2Of	Eomes,and
R14080	T18059	T18058	arg2Of	normalized,and
R14081	T18059	T18060	arg1Of	normalized,for
R14082	T18061	T18060	arg2Of	loading,for
R14083	T18061	T18062	arg1Of	loading,with
R14084	T18064	T18062	arg2Of	amount,with
R14085	T18064	T18063	arg1Of	amount,the
R14086	T18064	T18065	arg1Of	amount,of
R14087	T18067	T18065	arg2Of	Pol-II,of
R14088	T18067	T18066	arg1Of	Pol-II,RNA
R14089	T18067	T18068	arg2Of	Pol-II,detected
R1409	T1717	T1716	arg1Of	factors,transcription
R14090	T18068	T18069	arg1Of	detected,in
R14091	T18071	T18069	arg2Of	lane,in
R14092	T18071	T18070	arg1Of	lane,each
R1410	T1717	T1718	arg1Of	factors,function
R1411	T1661	T1659	arg2Of	cells,in
R1412	T1661	T1660	arg1Of	cells,T
R1413	T1717	T1721	arg1Of	factors,control
R1414	T1718	T1714	arg1Of	function,whether
R1415	T1663	T1662	arg2Of	impaired,have
R14158	T18192	T18190	arg1Of	material,Online
R14159	T18192	T18191	arg1Of	material,supplemental
R1416	T1663	T1668	arg1Of	impaired,","
R14160	T18194	T18193	arg1Of	S1,Fig.
R14161	T18194	T18195	arg1Of	S1,shows
R14162	T18197	T18195	arg2Of	characterization,shows
R14163	T18197	T18196	arg1Of	characterization,the
R14164	T18197	T18198	arg1Of	characterization,of
R14165	T18202	T18198	arg2Of	cells,of
R14166	T18202	T18199	arg1Of	cells,peripheral
R14167	T18202	T18200	arg1Of	cells,CD8+
R14168	T18202	T18201	arg1Of	cells,T
R14169	T18202	T18203	arg1Of	cells,from
R1417	T1664	T1663	arg2Of	expression,impaired
R14170	T18205	T18203	arg2Of	mice,from
R14171	T18205	T18204	arg1Of	mice,Runx3−/−
R14172	T18207	T18206	arg1Of	S2,Fig.
R14173	T18207	T18208	arg1Of	S2,shows
R14174	T18211	T18208	arg2Of	expression,shows
R14175	T18211	T18209	arg1Of	expression,effector
R14176	T18211	T18210	arg1Of	expression,protein
R14177	T18211	T18212	arg1Of	expression,by
R14178	T18214	T18213	arg1Of	WT,Runx3
R14179	T18214	T18215	arg1Of	WT,and
R1418	T1664	T1665	arg1Of	expression,of
R14180	T18215	T18212	arg2Of	and,by
R14181	T18215	T18218	arg1Of	and,at
R14182	T18217	T18215	arg2Of	cells,and
R14183	T18217	T18216	arg1Of	cells,KO
R14184	T18219	T18218	arg2Of	day,at
R14185	T18219	T18220	arg1Of	day,4
R14186	T18219	T18221	arg1Of	day,of
R14187	T18223	T18222	arg1Of	vitro,in
R14188	T18224	T18221	arg2Of	culture,of
R14189	T18224	T18223	arg1Of	culture,vitro
R1419	T1718	T1719	arg1Of	function,redundantly
R14190	T18226	T18225	arg1Of	sequences,Primer
R14191	T18226	T18227	arg2Of	sequences,used
R14192	T18226	T18231	arg1Of	sequences,are
R14193	T18226	T18232	arg2Of	sequences,shown
R14194	T18227	T18228	arg1Of	used,for
R14195	T18230	T18228	arg2Of	experiments,for
R14196	T18230	T18229	arg1Of	experiments,ChIP
R14197	T18232	T18231	arg2Of	shown,are
R14198	T18232	T18233	arg1Of	shown,in
R14199	T18235	T18233	arg2Of	S1,in
R1420	T1667	T1665	arg2Of	mediators,of
R14200	T18235	T18234	arg1Of	S1,Table
R14201	T18236	T18239	modOf	Online,is
R14202	T18236	T18243	arg1Of	Online,:
R14203	T18238	T18237	arg1Of	material,supplemental
R14204	T18238	T18239	arg1Of	material,is
R14205	T18238	T18240	arg1Of	material,available
R14206	T18240	T18239	arg2Of	available,is
R14207	T18240	T18241	arg1Of	available,at
R14208	T18242	T18241	arg2Of	http,at
R14209	T18244	T18236	arg1Of	//www.jem.org/cgi/content/full/jem.20081242/DC1,Online
R1421	T1667	T1666	arg1Of	mediators,cytolytic
R1422	T1718	T1720	modOf	function,to
R1423	T1668	T1674	arg1Of	",",and
R1424	T1718	T1727	arg1Of	function,and
R1425	T1669	T1668	arg2Of	manifest,","
R1426	T1721	T1720	arg1Of	control,to
R1427	T1672	T1669	arg2Of	activity,manifest
R1428	T1672	T1670	arg1Of	activity,poor
R1429	T1672	T1671	arg1Of	activity,cytolytic
R1430	T1674	T1673	arg1Of	and,","
R1431	T1675	T1674	arg2Of	fail,and
R1432	T1725	T1721	arg2Of	differentiation,control
R1433	T1677	T1675	arg2Of	control,fail
R1434	T1677	T1676	arg1Of	control,to
R1435	T1682	T1677	arg2Of	infection,control
R1436	T1682	T1678	arg1Of	infection,acute
R1437	T1682	T1679	arg1Of	infection,lymphocytic
R1438	T1682	T1680	arg1Of	infection,choriomeningitis
R1439	T1682	T1681	arg1Of	infection,virus
R1440	T1682	T1683	arg1Of	infection,(
R1441	T1684	T1683	arg2Of	9,(
R1442	T1725	T1722	arg1Of	differentiation,effector
R1443	T1685	T1683	arg3Of	),(
R1444	T1725	T1723	arg1Of	differentiation,CD8+ T
R1445	T1725	T1724	arg1Of	differentiation,cell
R1446	T1727	T1713	arg3Of	and,known
R1447	T1690	T1688	arg1Of	roles,the
R1448	T1690	T1689	arg1Of	roles,specific
R1449	T1690	T1691	arg1Of	roles,of
R14496	T18624	T18625	arg1Of	Kinetics,of
R14497	T18624	T18628	arg1Of	Kinetics,during
R14498	T18627	T18625	arg2Of	expression,of
R14499	T18627	T18626	arg1Of	expression,gene
R1450	T1690	T1695	arg1Of	roles,in
R14500	T18632	T18630	arg1Of	differentiation.,T
R14501	T18632	T18631	arg1Of	differentiation.,cell
R14502	T18632	T18633	arg1Of	differentiation.,(
R14503	T18634	T18633	arg2Of	A,(
R14504	T18635	T18633	arg3Of	),(
R14505	T18636	T18628	arg2Of	Kinetics,during
R14506	T18636	T18629	arg1Of	Kinetics,CD8+
R14507	T18636	T18632	arg1Of	Kinetics,differentiation.
R14508	T18636	T18637	arg1Of	Kinetics,of
R14509	T18638	T18639	arg1Of	Prf1,","
R1451	T1690	T1701	arg1Of	roles,have
R14510	T18639	T18641	arg1Of	",",","
R14511	T18640	T18639	arg2Of	Gzmb,","
R14512	T18641	T18647	arg1Of	",",and
R14513	T18642	T18641	arg2Of	Tbx21,","
R14514	T18642	T18643	arg1Of	Tbx21,(
R14515	T18644	T18643	arg2Of	T-bet,(
R14516	T18645	T18643	arg3Of	),(
R14517	T18647	T18637	arg2Of	and,of
R14518	T18647	T18646	arg1Of	and,","
R14519	T18647	T18651	arg1Of	and,in
R1452	T1690	T1704	arg1Of	roles,been
R14520	T18650	T18647	arg2Of	expression,and
R14521	T18650	T18648	arg1Of	expression,Eomes
R14522	T18650	T18649	arg1Of	expression,mRNA
R14523	T18656	T18651	arg2Of	cells,in
R14524	T18656	T18652	arg1Of	cells,differentiating
R14525	T18656	T18653	arg1Of	cells,P14
R14526	T18656	T18654	arg1Of	cells,CD8+
R14527	T18656	T18655	arg1Of	cells,T
R14528	T18656	T18657	arg2Of	cells,analyzed
R14529	T18660	T18657	arg1Of	blotting,analyzed
R1453	T1690	T1705	arg2Of	roles,resolved
R14530	T18660	T18658	arg2Of	blotting,by
R14531	T18660	T18659	arg1Of	blotting,Northern
R14532	T18661	T18662	arg1Of	RNA,from
R14533	T18661	T18667	arg1Of	RNA,was
R14534	T18661	T18668	arg2Of	RNA,used
R14535	T18666	T18662	arg2Of	cells,from
R14536	T18666	T18663	arg1Of	cells,day
R14537	T18666	T18664	arg1Of	cells,7
R14538	T18666	T18665	arg1Of	cells,Th1
R14539	T18668	T18667	arg2Of	used,was
R1454	T1692	T1693	arg1Of	T-bet,and
R14540	T18668	T18669	arg1Of	used,as
R14541	T18671	T18669	arg2Of	control,as
R14542	T18671	T18670	arg1Of	control,a
R14543	T18672	T18673	arg1Of	Sizes,of
R14544	T18672	T18676	arg1Of	Sizes,are
R14545	T18672	T18677	arg2Of	Sizes,indicated.
R14546	T18675	T18673	arg2Of	transcripts,of
R14547	T18675	T18674	arg1Of	transcripts,mRNA
R14548	T18677	T18676	arg2Of	indicated.,are
R14549	T18679	T18678	arg2Of	B,(
R1455	T1693	T1691	arg2Of	and,of
R14550	T18680	T18678	arg3Of	),(
R14551	T18681	T18677	arg3Of	Quantification,indicated.
R14552	T18681	T18678	arg1Of	Quantification,(
R14553	T18681	T18682	arg1Of	Quantification,of
R14554	T18681	T18686	arg1Of	Quantification,by
R14555	T18685	T18682	arg2Of	amounts,of
R14556	T18685	T18683	arg1Of	amounts,relative
R14557	T18685	T18684	arg1Of	amounts,mRNA
R14558	T18688	T18687	arg1Of	analysis.,phosphorimager
R14559	T18688	T18689	arg1Of	analysis.,(
R1456	T1694	T1693	arg2Of	Eomes,and
R14560	T18690	T18689	arg2Of	C,(
R14561	T18691	T18689	arg3Of	),(
R14562	T18692	T18686	arg2Of	Kinetics,by
R14563	T18692	T18688	arg1Of	Kinetics,analysis.
R14564	T18692	T18693	arg1Of	Kinetics,of
R14565	T18695	T18693	arg2Of	expression,of
R14566	T18695	T18694	arg1Of	expression,protein
R14567	T18695	T18696	arg1Of	expression,in
R14568	T18701	T18696	arg2Of	cells,in
R14569	T18701	T18697	arg1Of	cells,differentiating
R1457	T1727	T1726	arg1Of	and,","
R14570	T18701	T18698	arg1Of	cells,P14
R14571	T18701	T18699	arg1Of	cells,CD8+
R14572	T18701	T18700	arg1Of	cells,T
R14573	T18701	T18702	arg2Of	cells,analyzed
R14574	T18704	T18702	arg1Of	immunoblotting,analyzed
R14575	T18704	T18703	arg2Of	immunoblotting,by
R14576	T18705	T18706	arg1Of	Sizes,of
R14577	T18705	T18709	arg1Of	Sizes,are
R14578	T18705	T18710	arg2Of	Sizes,indicated.
R14579	T18708	T18706	arg2Of	bands,of
R1458	T1697	T1696	arg1Of	expansion,clonal
R14580	T18708	T18707	arg1Of	bands,protein
R14581	T18710	T18709	arg2Of	indicated.,are
R14582	T18712	T18711	arg2Of	D,(
R14583	T18713	T18711	arg3Of	),(
R14584	T18716	T18710	arg3Of	amounts,indicated.
R14585	T18716	T18711	arg1Of	amounts,(
R14586	T18716	T18714	arg1Of	amounts,Relative
R14587	T18716	T18715	arg1Of	amounts,protein
R14588	T18716	T18717	arg2Of	amounts,quantified
R14589	T18716	T18727	arg1Of	amounts,for
R1459	T1697	T1698	arg1Of	expansion,and
R14590	T18717	T18718	arg1Of	quantified,from
R14591	T18723	T18722	arg2Of	E,(
R14592	T18724	T18722	arg3Of	),(
R14593	T18726	T18718	arg2Of	staining,from
R14594	T18726	T18719	arg1Of	staining,the
R14595	T18726	T18720	arg1Of	staining,Western
R14596	T18726	T18721	arg1Of	staining,blots.
R14597	T18726	T18722	arg1Of	staining,(
R14598	T18726	T18725	arg1Of	staining,Intracellular
R14599	T18729	T18728	arg1Of	B,granzyme
R1460	T1728	T1727	arg2Of	whether,and
R14600	T18729	T18730	arg1Of	B,","
R14601	T18730	T18733	arg1Of	",",and
R14602	T18731	T18730	arg2Of	IFN-γ,","
R14603	T18733	T18727	arg2Of	and,for
R14604	T18733	T18732	arg1Of	and,","
R14605	T18734	T18733	arg2Of	TNF,and
R14606	T18737	T18735	arg1Of	staining,Granzyme
R14607	T18737	T18736	arg1Of	staining,B
R14608	T18737	T18738	arg1Of	staining,was
R14609	T18737	T18739	arg1Of	staining,specific
R1461	T1698	T1695	arg2Of	and,in
R14610	T18739	T18738	arg2Of	specific,was
R14611	T18739	T18741	arg1Of	specific,to
R14612	T18741	T18740	arg1Of	to,relative
R14613	T18744	T18741	arg2Of	control,to
R14614	T18744	T18742	arg1Of	control,an
R14615	T18744	T18743	arg1Of	control,isotype
R14616	T18744	T18745	arg1Of	control,(
R14617	T18747	T18745	arg2Of	depicted,(
R14618	T18747	T18746	arg1Of	depicted,not
R14619	T18748	T18745	arg3Of	),(
R1462	T1700	T1698	arg2Of	differentiation,and
R14620	T18749	T18750	arg1Of	Cells,were
R14621	T18749	T18751	arg2Of	Cells,restimulated
R14622	T18751	T18750	arg2Of	restimulated,were
R14623	T18751	T18752	arg1Of	restimulated,with
R14624	T18753	T18754	arg1Of	PMA,and
R14625	T18754	T18752	arg2Of	and,with
R14626	T18754	T18756	arg1Of	and,for
R14627	T18755	T18754	arg2Of	ionomycin,and
R14628	T18758	T18757	arg1Of	h.,4
R14629	T18758	T18759	arg1Of	h.,(
R1463	T1729	T1730	arg1Of	they,do
R14630	T18760	T18759	arg2Of	F,(
R14631	T18761	T18759	arg3Of	),(
R14632	T18762	T18758	arg1Of	FACS-based,h.
R14633	T18763	T18756	arg2Of	assay,for
R14634	T18763	T18762	arg1Of	assay,FACS-based
R14635	T18763	T18764	modOf	assay,to
R14636	T18765	T18764	arg1Of	measure,to
R14637	T18765	T18773	arg1Of	measure,against
R14638	T18767	T18765	arg2Of	activity,measure
R14639	T18767	T18766	arg1Of	activity,cytolytic
R1464	T1700	T1699	arg1Of	differentiation,CTL
R14640	T18767	T18768	arg1Of	activity,of
R14641	T18772	T18768	arg2Of	cells,of
R14642	T18772	T18769	arg1Of	cells,P14
R14643	T18772	T18770	arg1Of	cells,CD8+
R14644	T18772	T18771	arg1Of	cells,T
R14645	T18775	T18773	arg2Of	targets,against
R14646	T18775	T18774	arg1Of	targets,EL4
R14647	T18775	T18776	arg2Of	targets,loaded
R14648	T18776	T18777	arg1Of	loaded,with
R14649	T18778	T18779	arg1Of	0,(
R1465	T1730	T1728	arg2Of	do,whether
R14650	T18778	T18782	arg1Of	0,or
R14651	T18780	T18779	arg2Of	−,(
R14652	T18781	T18779	arg3Of	),(
R14653	T18782	T18777	arg2Of	or,with
R14654	T18783	T18782	arg2Of	1,or
R14655	T18783	T18784	arg1Of	1,(
R14656	T18785	T18784	arg2Of	+,(
R14657	T18786	T18784	arg3Of	),(
R14658	T18789	T18765	arg3Of	peptide,measure
R14659	T18789	T18787	arg1Of	peptide,μM
R1466	T1730	T1731	arg1Of	do,so
R14660	T18789	T18788	arg1Of	peptide,Gp33
R14661	T18789	T18790	arg1Of	peptide,(
R14662	T18792	T18790	arg2Of	ratio,(
R14663	T18792	T18791	arg1Of	ratio,effector-to-target
R14664	T18792	T18795	arg1Of	ratio,:
R14665	T18795	T18793	arg1Of	:,=
R14666	T18795	T18794	arg1Of	:,5
R14667	T18795	T18796	arg1Of	:,1
R14668	T18797	T18790	arg3Of	),(
R14669	T18798	T18799	arg1Of	Percentage,of
R1467	T1730	T1732	arg1Of	do,directly
R14670	T18798	T18807	arg1Of	Percentage,in
R14671	T18798	T18817	arg1Of	Percentage,was
R14672	T18798	T18818	arg2Of	Percentage,determined
R14673	T18806	T18799	arg2Of	cells,of
R14674	T18806	T18800	arg1Of	cells,Annexin
R14675	T18806	T18801	arg1Of	cells,V+
R14676	T18806	T18802	arg1Of	cells,(
R14677	T18806	T18803	arg1Of	cells,apoptotic
R14678	T18806	T18804	arg1Of	cells,)
R14679	T18806	T18805	arg1Of	cells,target
R1468	T1730	T1733	arg1Of	do,by
R14680	T18812	T18807	arg2Of	population,in
R14681	T18812	T18808	arg1Of	population,the
R14682	T18812	T18809	arg1Of	population,CD8-negative
R14683	T18812	T18810	arg1Of	population,EL4
R14684	T18812	T18811	arg1Of	population,target
R14685	T18812	T18813	arg1Of	population,(
R14686	T18815	T18813	arg2Of	plots,(
R14687	T18815	T18814	arg1Of	plots,dot
R14688	T18816	T18813	arg3Of	),(
R14689	T18818	T18817	arg2Of	determined,was
R1469	T1734	T1733	arg2Of	targeting,by
R14690	T18818	T18819	arg1Of	determined,(
R14691	T18820	T18819	arg2Of	histograms,(
R14692	T18821	T18819	arg3Of	),(
R14693	T18823	T18822	arg1Of	activity,Cytolytic
R14694	T18823	T18824	arg1Of	activity,was
R14695	T18823	T18825	arg2Of	activity,blocked
R14696	T18825	T18824	arg2Of	blocked,was
R14697	T18825	T18826	arg1Of	blocked,by
R14698	T18825	T18836	arg1Of	blocked,","
R14699	T18825	T18837	modOf	blocked,confirming
R1470	T1737	T1735	arg1Of	cytokine,specific
R14700	T18827	T18826	arg2Of	incubation,by
R14701	T18827	T18828	arg1Of	incubation,with
R14702	T18829	T18830	arg1Of	2,mM
R14703	T18831	T18828	arg2Of	EGTA,with
R14704	T18831	T18829	arg1Of	EGTA,2
R14705	T18831	T18832	arg1Of	EGTA,(
R14706	T18834	T18832	arg2Of	depicted,(
R14707	T18834	T18833	arg1Of	depicted,not
R14708	T18835	T18832	arg3Of	),(
R14709	T18838	T18837	arg2Of	involvement,confirming
R1471	T1799	T1798	arg1Of	expression,its
R14710	T18838	T18839	arg1Of	involvement,of
R14711	T18842	T18841	arg1Of	exocytosis,granule
R14712	T18842	T18843	arg1Of	exocytosis,(
R14713	T18845	T18843	arg2Of	B,(
R14714	T18845	T18844	arg1Of	B,perforin–granzyme
R14715	T18846	T18843	arg3Of	),(
R14716	T18847	T18839	arg2Of	pathway,of
R14717	T18847	T18840	arg1Of	pathway,the
R14718	T18847	T18842	arg1Of	pathway,exocytosis
R14719	T18848	T18849	arg1Of	Data,are
R1472	T1799	T1800	arg1Of	expression,is
R14720	T18848	T18850	arg1Of	Data,representative
R14721	T18850	T18849	arg2Of	representative,are
R14722	T18850	T18851	arg1Of	representative,of
R14723	T18854	T18852	arg1Of	five,at
R14724	T18854	T18853	arg1Of	five,least
R14725	T18856	T18855	arg2Of	A–E,(
R14726	T18856	T18858	arg1Of	A–E,or
R14727	T18857	T18855	arg3Of	),(
R14728	T18859	T18858	arg2Of	three,or
R14729	T18861	T18860	arg2Of	F,(
R1473	T1799	T1801	arg2Of	expression,up-regulated
R14730	T18862	T18860	arg3Of	),(
R14731	T18864	T18851	arg2Of	experiments,of
R14732	T18864	T18854	arg1Of	experiments,five
R14733	T18864	T18855	arg1Of	experiments,(
R14734	T18864	T18859	arg1Of	experiments,three
R14735	T18864	T18860	arg1Of	experiments,(
R14736	T18864	T18863	arg1Of	experiments,independent
R1474	T1801	T1800	arg2Of	up-regulated,is
R1475	T1801	T1802	arg1Of	up-regulated,during
R1476	T1801	T1807	arg1Of	up-regulated,and
R1477	T1737	T1736	arg1Of	cytokine,effector
R1478	T1737	T1738	arg1Of	cytokine,and
R1479	T1804	T1803	arg1Of	cell,Th1
R1480	T1738	T1734	arg2Of	and,targeting
R1481	T1805	T1802	arg2Of	differentiation,during
R1482	T1805	T1804	arg1Of	differentiation,cell
R1483	T1738	T1742	arg1Of	and,","
R1484	T1807	T1788	arg1Of	and,Although
R1485	T1807	T1797	arg1Of	and,","
R1486	T1807	T1806	arg1Of	and,","
R1487	T1808	T1809	arg1Of	Runx3,influences
R1488	T1738	T1750	arg1Of	and,","
R1489	T1809	T1807	arg2Of	influences,and
R1490	T1809	T1815	arg1Of	influences,through
R1491	T1741	T1738	arg2Of	proteins,and
R1492	T1812	T1813	arg1Of	differentiation,and
R1493	T1813	T1809	arg2Of	and,influences
R1494	T1813	T1810	arg1Of	and,Th1
R1495	T1813	T1811	arg1Of	and,cell
R1496	T1814	T1813	arg2Of	function,and
R1497	T1741	T1739	arg1Of	proteins,cytolytic
R1498	T1817	T1815	arg2Of	regulation,through
R1499	T1817	T1816	arg1Of	regulation,direct
R1500	T1817	T1818	arg1Of	regulation,of
R1501	T1820	T1818	arg2Of	genes,of
R1502	T1820	T1819	arg1Of	genes,the Il4 and Ifng cytokine
R1503	T1820	T1821	arg1Of	genes,(
R1504	T1741	T1740	arg1Of	proteins,"genes. Runx"
R1505	T1744	T1742	arg2Of	family,","
R1506	T1822	T1821	arg2Of	"15, 16",(
R1507	T1823	T1821	arg3Of	),(
R1508	T1825	T1824	arg2Of	contrast,In
R1509	T1744	T1743	arg1Of	family,a
R1510	T1744	T1745	arg1Of	family,of
R1511	T1749	T1745	arg2Of	factors,of
R1512	T1830	T1827	arg1Of	proteins,all
R15127	T19474	T19475	arg1Of	Regulation,of
R15128	T19474	T19488	arg1Of	Regulation,in
R15129	T19476	T19477	arg1Of	perforin,","
R1513	T1830	T1828	arg1Of	proteins,three
R15130	T19477	T19481	arg1Of	",",and
R15131	T19479	T19477	arg2Of	B,","
R15132	T19479	T19478	arg1Of	B,granzyme
R15133	T19481	T19475	arg2Of	and,of
R15134	T19481	T19480	arg1Of	and,","
R15135	T19481	T19484	arg1Of	and,by
R15136	T19483	T19481	arg2Of	expression,and
R15137	T19483	T19482	arg1Of	expression,IFN-γ
R15138	T19485	T19486	arg1Of	T-bet,and
R15139	T19486	T19484	arg2Of	and,by
R1514	T1830	T1829	arg1Of	proteins,Runx
R15140	T19487	T19486	arg2Of	Eomes,and
R15141	T19489	T19488	arg2Of	differentiating,in
R15142	T19489	T19496	arg1Of	differentiating,by
R15143	T19490	T19491	arg1Of	CTLs.,(
R15144	T19492	T19491	arg2Of	A,(
R15145	T19493	T19491	arg3Of	),(
R15146	T19495	T19489	arg2Of	expression,differentiating
R15147	T19495	T19490	arg1Of	expression,CTLs.
R15148	T19495	T19494	arg1Of	expression,IFN-γ
R15149	T19497	T19498	arg1Of	WT,(
R1515	T1830	T1831	arg1Of	proteins,are
R15150	T19497	T19501	arg1Of	WT,and
R15151	T19499	T19498	arg2Of	Tbx21+/+,(
R15152	T19500	T19498	arg3Of	),(
R15153	T19501	T19496	arg2Of	and,by
R15154	T19504	T19503	arg2Of	Tbx21−/−,(
R15155	T19505	T19503	arg3Of	),(
R15156	T19507	T19501	arg2Of	cells,and
R15157	T19507	T19502	arg1Of	cells,T-bet–deficient
R15158	T19507	T19503	arg1Of	cells,(
R15159	T19507	T19506	arg1Of	cells,T
R1516	T1830	T1832	arg2Of	proteins,expressed
R15160	T19511	T19508	arg1Of	cells,Naive
R15161	T19511	T19509	arg1Of	cells,CD8+
R15162	T19511	T19510	arg1Of	cells,T
R15163	T19511	T19513	arg1Of	cells,or
R15164	T19513	T19512	arg1Of	or,","
R15165	T19513	T19524	arg1Of	or,were
R15166	T19513	T19525	arg2Of	or,restimulated
R15167	T19514	T19513	arg2Of	cells,or
R15168	T19514	T19515	arg2Of	cells,activated
R15169	T19514	T19517	arg2Of	cells,cultured
R1517	T1832	T1824	arg1Of	expressed,In
R15170	T19515	T19516	arg1Of	activated,and
R15171	T19517	T19516	arg2Of	cultured,and
R15172	T19517	T19518	arg1Of	cultured,for
R15173	T19519	T19520	arg1Of	4,or
R15174	T19521	T19520	arg2Of	6,or
R15175	T19522	T19518	arg2Of	d,for
R15176	T19522	T19519	arg1Of	d,4
R15177	T19522	T19521	arg1Of	d,6
R15178	T19525	T19523	arg1Of	restimulated,","
R15179	T19525	T19524	arg2Of	restimulated,were
R1518	T1832	T1826	arg1Of	expressed,","
R15180	T19525	T19526	arg1Of	restimulated,with
R15181	T19525	T19530	arg1Of	restimulated,for
R15182	T19525	T19534	arg1Of	restimulated,and
R15183	T19527	T19528	arg1Of	PMA,and
R15184	T19528	T19526	arg2Of	and,with
R15185	T19529	T19528	arg2Of	ionomycin,and
R15186	T19532	T19530	arg2Of	h,for
R15187	T19532	T19531	arg1Of	h,6
R15188	T19534	T19533	arg1Of	and,","
R15189	T19536	T19535	arg1Of	expression,IFN-γ
R1519	T1832	T1831	arg2Of	expressed,are
R15190	T19536	T19537	arg1Of	expression,was
R15191	T19536	T19538	arg2Of	expression,assessed
R15192	T19538	T19534	arg2Of	assessed,and
R15193	T19538	T19537	arg2Of	assessed,was
R15194	T19541	T19538	arg1Of	staining,assessed
R15195	T19541	T19539	arg2Of	staining,by
R15196	T19541	T19540	arg1Of	staining,intracellular
R15197	T19542	T19543	arg1Of	Numbers,show
R15198	T19545	T19543	arg2Of	percentage,show
R15199	T19545	T19544	arg1Of	percentage,the
R1520	T1832	T1833	arg1Of	expressed,in
R15200	T19545	T19546	arg1Of	percentage,of
R15201	T19545	T19549	arg1Of	percentage,(
R15202	T19548	T19546	arg2Of	cells.,of
R15203	T19548	T19547	arg1Of	cells.,IFN-γ+
R15204	T19550	T19549	arg2Of	B,(
R15205	T19551	T19549	arg3Of	),(
R15206	T19554	T19543	arg3Of	analysis,show
R15207	T19554	T19552	arg1Of	analysis,Northern
R15208	T19554	T19553	arg1Of	analysis,blot
R15209	T19554	T19555	arg1Of	analysis,of
R1521	T1832	T1841	arg1Of	expressed,and
R15210	T19556	T19557	arg1Of	Prf1,and
R15211	T19558	T19557	arg2Of	GzmB,and
R15212	T19560	T19555	arg2Of	expression,of
R15213	T19560	T19556	arg1Of	expression,Prf1
R15214	T19560	T19558	arg1Of	expression,GzmB
R15215	T19560	T19559	arg1Of	expression,mRNA
R15216	T19560	T19561	arg1Of	expression,in
R15217	T19562	T19563	arg1Of	WT,or
R15218	T19564	T19563	arg2Of	T-bet–deficient,or
R15219	T19567	T19561	arg2Of	cells,in
R1522	T1836	T1833	arg2Of	cells,in
R15220	T19567	T19562	arg1Of	cells,WT
R15221	T19567	T19564	arg1Of	cells,T-bet–deficient
R15222	T19567	T19565	arg1Of	cells,CD8+
R15223	T19567	T19566	arg1Of	cells,T
R15224	T19567	T19568	arg2Of	cells,activated
R15225	T19567	T19572	arg1Of	cells,uninfected
R15226	T19567	T19577	arg2Of	cells,transduced
R15227	T19568	T19569	arg1Of	activated,and
R15228	T19569	T19576	arg1Of	and,or
R15229	T19572	T19569	arg2Of	uninfected,and
R1523	T1836	T1834	arg1Of	cells,mature
R15230	T19572	T19570	arg1Of	uninfected,either
R15231	T19572	T19571	arg1Of	uninfected,left
R15232	T19572	T19573	arg1Of	uninfected,(
R15233	T19574	T19573	arg2Of	uninf,(
R15234	T19575	T19573	arg3Of	),(
R15235	T19577	T19576	arg2Of	transduced,or
R15236	T19577	T19578	arg1Of	transduced,with
R15237	T19579	T19578	arg2Of	retroviruses,with
R15238	T19579	T19580	arg1Of	retroviruses,expressing
R15239	T19581	T19582	arg1Of	Eomes-VP16,(
R1524	T1836	T1835	arg1Of	cells,CD8+ T
R15240	T19581	T19585	arg1Of	Eomes-VP16,or
R15241	T19583	T19582	arg2Of	Eo-VP16,(
R15242	T19584	T19582	arg3Of	),(
R15243	T19585	T19580	arg2Of	or,expressing
R15244	T19589	T19585	arg2Of	cassette,or
R15245	T19589	T19586	arg1Of	cassette,an
R15246	T19589	T19587	arg1Of	cassette,empty
R15247	T19589	T19588	arg1Of	cassette,IRES-GFP
R15248	T19589	T19590	arg1Of	cassette,(
R15249	T19591	T19590	arg2Of	GFP,(
R1525	T1836	T1837	arg1Of	cells,(
R15250	T19592	T19590	arg3Of	),(
R15251	T19595	T19593	arg1Of	RNA,Total
R15252	T19595	T19594	arg1Of	RNA,cellular
R15253	T19595	T19596	arg1Of	RNA,was
R15254	T19595	T19597	arg2Of	RNA,analyzed
R15255	T19597	T19596	arg2Of	analyzed,was
R15256	T19597	T19598	arg1Of	analyzed,on
R15257	T19599	T19598	arg2Of	day,on
R15258	T19599	T19600	arg1Of	day,6
R15259	T19599	T19601	arg1Of	day,of
R1526	T1838	T1837	arg2Of	"10, 12",(
R15260	T19602	T19601	arg2Of	culture,of
R15261	T19604	T19603	arg1Of	frequency,The
R15262	T19604	T19605	arg1Of	frequency,of
R15263	T19604	T19608	arg1Of	frequency,in
R15264	T19604	T19611	arg1Of	frequency,was
R15265	T19604	T19612	arg1Of	frequency,equivalent
R15266	T19607	T19605	arg2Of	cells,of
R15267	T19607	T19606	arg2Of	cells,transduced
R15268	T19610	T19608	arg2Of	cultures,in
R15269	T19610	T19609	arg1Of	cultures,the
R1527	T1749	T1746	arg1Of	factors,three
R15270	T19612	T19611	arg2Of	equivalent,was
R15271	T19612	T19613	arg1Of	equivalent,for
R15272	T19615	T19613	arg2Of	constructs,for
R15273	T19615	T19614	arg1Of	constructs,both
R15274	T19617	T19618	arg1Of	∼65–70,%
R15275	T19620	T19616	arg2Of	cells,(
R15276	T19620	T19617	arg1Of	cells,∼65–70
R15277	T19620	T19619	arg1Of	cells,GFP+
R15278	T19626	T19625	arg2Of	(,.
R15279	T19627	T19625	arg3Of	C,.
R1528	T1839	T1837	arg3Of	),(
R15280	T19629	T19628	arg1Of	m,) Granzy
R15281	T19629	T19630	arg1Of	m,e B
R15282	T19630	T19625	arg1Of	e B,.
R15283	T19632	T19630	arg2Of	FN-γ expre,e B
R15284	T19632	T19631	arg1Of	FN-γ expre,and I
R15285	T19634	T19635	arg1Of	ion by T,bx2
R15286	T19636	T19635	arg2Of	1+/+ and,bx2
R15287	T19638	T19633	arg2Of	bx21−,ss
R15288	T19638	T19634	arg1Of	bx21−,ion by T
R15289	T19638	T19636	arg1Of	bx21−,1+/+ and
R1529	T1749	T1747	arg1Of	factors,DNA-binding
R15290	T19638	T19637	arg1Of	bx21−,T
R15291	T19638	T19639	arg2Of	bx21−,/− T cel
R15292	T19638	T19643	arg1Of	bx21−,mula
R15293	T19638	T19644	arg1Of	bx21−,ted
R15294	T19638	T19645	arg1Of	bx21−,cell
R15295	T19638	T19646	arg2Of	bx21−,s that h
R15296	T19639	T19640	arg1Of	/− T cel,ls
R15297	T19642	T19640	arg2Of	resti,ls
R15298	T19642	T19641	arg2Of	resti,analyzed in
R15299	T19646	T19644	arg2Of	s that h,ted
R1530	T1749	T1748	arg1Of	factors,transcription
R15300	T19646	T19645	arg2Of	s that h,cell
R15301	T19646	T19647	arg1Of	s that h,ad
R15302	T19649	T19648	arg1Of	ee,b
R15303	T19649	T19650	arg1Of	ee,n
R15304	T19651	T19650	arg2Of	c,n
R15305	T19652	T19650	arg3Of	u,n
R15306	T19654	T19647	arg2Of	d for 5 d.,ad
R15307	T19654	T19649	arg1Of	d for 5 d.,ee
R15308	T19654	T19653	arg1Of	d for 5 d.,lture
R15309	T19656	T19655	arg2Of	) IFN,(D
R1531	T1841	T1840	arg1Of	and,","
R15310	T19656	T19657	arg2Of	) IFN,-γ product
R15311	T19657	T19658	arg1Of	-γ product,ion
R15312	T19659	T19660	arg1Of	by cell,s
R15313	T19660	T19658	arg2Of	s ,ion
R15314	T19663	T19662	arg2Of	ed ,c
R15315	T19664	T19662	arg3Of	w,c
R15316	T19665	T19660	arg2Of	ith Eo-VP16 ,s
R15317	T19665	T19661	arg1Of	ith Eo-VP16 ,transdu
R15318	T19665	T19662	arg1Of	ith Eo-VP16 ,c
R15319	T19665	T19666	arg1Of	ith Eo-VP16 ,o
R1532	T1753	T1751	arg1Of	differentiation,control
R15320	T19665	T19669	arg2Of	ith Eo-VP16 ,ontrol (
R15321	T19667	T19666	arg2Of	r ,o
R15322	T19668	T19666	arg3Of	c,o
R15323	T19669	T19670	arg1Of	ontrol (,GF
R15324	T19669	T19673	arg1Of	ontrol (,etrov
R15325	T19671	T19670	arg2Of	P) ,GF
R15326	T19671	T19672	arg1Of	P) ,r
R15327	T19675	T19673	arg2Of	r,etrov
R15328	T19675	T19674	arg1Of	r,i
R15329	T19675	T19676	arg1Of	r,us
R1533	T1844	T1842	arg1Of	cells,Runx3-deficient
R15330	T19677	T19676	arg2Of	es (RV) measu,us
R15331	T19677	T19678	arg1Of	es (RV) measu,red
R15332	T19679	T19680	arg1Of	on ,day
R15333	T19680	T19678	arg2Of	day,red
R15334	T19681	T19680	arg2Of	4 after 6,day
R15335	T19682	T19683	arg1Of	Numbers,show
R15336	T19685	T19683	arg2Of	percentage,show
R15337	T19685	T19684	arg1Of	percentage,the
R15338	T19685	T19686	arg1Of	percentage,of
R15339	T19689	T19686	arg2Of	cells,of
R1534	T1844	T1843	arg1Of	cells,CD8+ T
R15340	T19689	T19687	arg1Of	cells,GFP+
R15341	T19689	T19688	arg1Of	cells,IFN-γ+
R15342	T19690	T19691	arg1Of	Results,are
R15343	T19690	T19692	arg1Of	Results,representative
R15344	T19692	T19691	arg2Of	representative,are
R15345	T19692	T19693	arg1Of	representative,of
R15346	T19694	T19695	arg1Of	three,(
R15347	T19694	T19700	arg1Of	three,or
R15348	T19696	T19697	arg1Of	A,and
R15349	T19697	T19695	arg2Of	and,(
R1535	T1844	T1845	arg1Of	cells,show
R15350	T19698	T19697	arg2Of	C,and
R15351	T19699	T19695	arg3Of	),(
R15352	T19700	T19702	arg1Of	or,(
R15353	T19701	T19700	arg2Of	two,or
R15354	T19703	T19704	arg1Of	B,and
R15355	T19704	T19702	arg2Of	and,(
R15356	T19705	T19704	arg2Of	D,and
R15357	T19706	T19702	arg3Of	),(
R15358	T19708	T19693	arg2Of	experiments,of
R15359	T19708	T19694	arg1Of	experiments,three
R1536	T1845	T1841	arg2Of	show,and
R15360	T19708	T19701	arg1Of	experiments,two
R15361	T19708	T19707	arg1Of	experiments,independent
R1537	T1753	T1752	arg1Of	differentiation,thymocyte
R1538	T1848	T1845	arg2Of	activity,show
R1539	T1848	T1846	arg2Of	activity,reduced
R1540	T1848	T1847	arg1Of	activity,cytolytic
R1541	T1848	T1849	arg1Of	activity,(
R1542	T1753	T1754	arg1Of	differentiation,and
R1543	T1850	T1849	arg2Of	"12, 13",(
R1544	T1754	T1750	arg2Of	and,","
R1545	T1851	T1849	arg3Of	),(
R1546	T1852	T1854	arg1Of	We,tested
R1547	T1758	T1754	arg2Of	decision,and
R1548	T1854	T1853	arg1Of	tested,therefore
R1549	T1856	T1857	arg1Of	Runx3,influenced
R1550	T1857	T1854	arg2Of	influenced,tested
R1551	T1857	T1855	arg1Of	influenced,whether
R1552	T1857	T1863	arg1Of	influenced,report
R1553	T1758	T1755	arg1Of	decision,the
R1554	T1758	T1756	arg1Of	decision,CD4/CD8
R1555	T1758	T1757	arg1Of	decision,lineage
R1556	T1861	T1857	arg2Of	"differentiation. In",influenced
R1557	T1861	T1858	arg1Of	"differentiation. In",cytolytic
R1558	T1861	T1859	arg1Of	"differentiation. In",T
R1559	T1861	T1860	arg1Of	"differentiation. In",cell
R1560	T1758	T1759	arg1Of	decision,(
R1561	T1863	T1862	arg1Of	report,this
R1562	T1863	T1866	modOf	report,show
R1563	T1760	T1759	arg2Of	10–13,(
R1564	T1865	T1866	arg1Of	we,show
R1565	T1866	T1864	arg1Of	show,","
R1566	T1761	T1759	arg3Of	),(
R1567	T1868	T1869	arg1Of	Runx3,and
R1568	T1870	T1869	arg2Of	T-box,and
R1569	T1762	T1763	arg1Of	Runx3,and
R1570	T1871	T1868	arg1Of	factors,Runx3
R1571	T1871	T1870	arg1Of	factors,T-box
R1572	T1871	T1873	arg1Of	factors,regulate
R1573	T1873	T1866	arg2Of	regulate,show
R1574	T1873	T1867	arg1Of	regulate,that
R1575	T1873	T1872	arg1Of	regulate,synergistically
R1576	T1763	T1766	arg1Of	and,are
R1577	T1875	T1876	arg1Of	differentiation,and
R1578	T1876	T1873	arg2Of	and,regulate
R1579	T1876	T1874	arg1Of	and,CTL
R1580	T1763	T1767	arg2Of	and,expressed
R1581	T1877	T1876	arg2Of	function,and
R1582	T1765	T1763	arg2Of	mRNA,and
R1583	T1878	T1879	arg1Of	T-bet,is
R1584	T1878	T1880	arg2Of	T-bet,induced
R1585	T1878	T1886	arg1Of	T-bet,is
R1586	T1878	T1887	arg2Of	T-bet,required
R1587	T1880	T1879	arg2Of	induced,is
R1588	T1880	T1881	arg1Of	induced,quickly
R1589	T1880	T1882	arg1Of	induced,upon
R1590	T1880	T1885	arg1Of	induced,and
R1591	T1765	T1764	arg1Of	mRNA,perforin
R1592	T1884	T1882	arg2Of	stimulation,upon
R1593	T1767	T1766	arg2Of	expressed,are
R1594	T1884	T1883	arg1Of	stimulation,TCR
R1595	T1885	T1898	arg1Of	and,whereas
R1596	T1767	T1768	arg1Of	expressed,by
R1597	T1767	T1782	arg1Of	expressed,in
R1598	T1887	T1885	arg2Of	required,and
R1599	T1887	T1886	arg2Of	required,is
R1600	T1887	T1888	arg1Of	required,for
R1601	T1890	T1888	arg2Of	programming,for
R1602	T1890	T1889	arg1Of	programming,early
R1603	T1890	T1891	arg1Of	programming,of
R1604	T1893	T1891	arg2Of	production,of
R1605	T1893	T1892	arg1Of	production,cytokine
R1606	T1893	T1894	arg1Of	production,(
R1607	T1768	T1780	arg1Of	by,but
R1608	T1771	T1770	arg2Of	DP,(
R1609	T1772	T1770	arg3Of	),(
R1610	T1895	T1894	arg2Of	17,(
R1611	T1896	T1894	arg3Of	),(
R1612	T1773	T1769	arg1Of	thymocytes,double-positive
R1613	T1898	T1897	arg1Of	whereas,","
R1614	T1899	T1900	arg1Of	Eomes,is
R1615	T1899	T1901	arg2Of	Eomes,induced
R1616	T1899	T1906	arg1Of	Eomes,sustains
R1617	T1773	T1770	arg1Of	thymocytes,(
R1618	T1773	T1774	arg1Of	thymocytes,and
R1619	T1901	T1900	arg2Of	induced,is
R1620	T1901	T1902	arg1Of	induced,later
R1621	T1901	T1903	arg1Of	induced,during
R1622	T1901	T1905	arg1Of	induced,and
R1623	T1774	T1768	arg2Of	and,by
R1624	T1904	T1903	arg2Of	differentiation,during
R1625	T1905	T1898	arg2Of	and,whereas
R1626	T1777	T1776	arg2Of	SP,(
R1627	T1906	T1905	arg2Of	sustains,and
R1628	T1908	T1906	arg2Of	expression,sustains
R1629	T1908	T1907	arg1Of	expression,IFN-γ
R1630	T1909	T1910	arg1Of	Runx3,is
R1631	T1909	T1911	arg2Of	Runx3,required
R1632	T1778	T1776	arg3Of	),(
R1633	T1911	T1910	arg2Of	required,is
R16331	T21280	T21281	arg1Of	Runx3,controls
R16332	T21282	T21281	arg2Of	Eomes,controls
R16333	T21282	T21283	arg1Of	Eomes,","
R16334	T21284	T21285	arg1Of	perforin,","
R16335	T21285	T21289	arg1Of	",",and
R16336	T21287	T21285	arg2Of	B,","
R16337	T21287	T21286	arg1Of	B,granzyme
R16338	T21289	T21283	arg2Of	and,","
R16339	T21289	T21288	arg1Of	and,","
R1634	T1911	T1912	arg1Of	required,for
R16340	T21289	T21292	arg1Of	and,in
R16341	T21291	T21289	arg2Of	expression,and
R16342	T21291	T21290	arg1Of	expression,IFN-γ
R16343	T21294	T21292	arg2Of	CTLs,in
R16344	T21294	T21293	arg1Of	CTLs,effector
R16345	T21295	T21296	arg1Of	Runx3+/+,or
R16346	T21297	T21296	arg2Of	Runx3−/−,or
R16347	T21300	T21295	arg1Of	cells,Runx3+/+
R16348	T21300	T21297	arg1Of	cells,Runx3−/−
R16349	T21300	T21298	arg1Of	cells,CD8+
R1635	T1911	T1918	arg1Of	required,and
R16350	T21300	T21299	arg1Of	cells,T
R16351	T21300	T21301	arg1Of	cells,were
R16352	T21300	T21302	arg2Of	cells,activated
R16353	T21300	T21304	arg2Of	cells,transduced
R16354	T21302	T21303	arg1Of	activated,and
R16355	T21303	T21301	arg2Of	and,were
R16356	T21304	T21303	arg2Of	transduced,and
R16357	T21304	T21305	arg1Of	transduced,with
R16358	T21306	T21305	arg2Of	retroviruses,with
R16359	T21306	T21307	arg1Of	retroviruses,bearing
R1636	T1913	T1914	arg1Of	Eomes,and
R16360	T21306	T21317	arg1Of	retroviruses,encoding
R16361	T21307	T21315	arg1Of	bearing,or
R16362	T21311	T21307	arg2Of	cassette,bearing
R16363	T21311	T21308	arg1Of	cassette,an
R16364	T21311	T21309	arg1Of	cassette,empty
R16365	T21311	T21310	arg1Of	cassette,IRES-GFP
R16366	T21311	T21312	arg1Of	cassette,(
R16367	T21313	T21312	arg2Of	GFP,(
R16368	T21314	T21312	arg3Of	),(
R16369	T21317	T21315	arg2Of	encoding,or
R1637	T1779	T1774	arg2Of	thymocytes,and
R16370	T21317	T21316	arg1Of	encoding,also
R16371	T21318	T21319	arg1Of	Eomes-VP16,(
R16372	T21318	T21322	arg1Of	Eomes-VP16,or
R16373	T21320	T21319	arg2Of	Eo-VP16,(
R16374	T21321	T21319	arg3Of	),(
R16375	T21322	T21317	arg2Of	or,encoding
R16376	T21323	T21322	arg2Of	Myc-Runx3,or
R16377	T21323	T21324	arg1Of	Myc-Runx3,(
R16378	T21325	T21324	arg2Of	Runx3,(
R16379	T21326	T21324	arg3Of	),(
R1638	T1914	T1912	arg2Of	and,for
R16380	T21328	T21327	arg1Of	frequency,The
R16381	T21328	T21329	arg1Of	frequency,of
R16382	T21328	T21332	arg1Of	frequency,in
R16383	T21328	T21335	arg1Of	frequency,was
R16384	T21328	T21336	arg1Of	frequency,equivalent
R16385	T21331	T21329	arg2Of	cells,of
R16386	T21331	T21330	arg2Of	cells,transduced
R16387	T21334	T21332	arg2Of	cultures,in
R16388	T21334	T21333	arg1Of	cultures,the
R16389	T21336	T21335	arg2Of	equivalent,was
R1639	T1916	T1914	arg2Of	expression,and
R16390	T21336	T21337	arg1Of	equivalent,for
R16391	T21339	T21337	arg2Of	constructs,for
R16392	T21339	T21338	arg1Of	constructs,all
R16393	T21339	T21340	arg1Of	constructs,(
R16394	T21341	T21342	arg1Of	∼75–90,%
R16395	T21344	T21340	arg2Of	cells,(
R16396	T21344	T21341	arg1Of	cells,∼75–90
R16397	T21344	T21343	arg1Of	cells,GFP+
R16398	T21344	T21345	arg1Of	cells,;
R16399	T21344	T21347	arg2Of	cells,depicted
R1640	T1916	T1915	arg1Of	expression,perforin
R16400	T21347	T21346	arg1Of	depicted,not
R16401	T21348	T21340	arg3Of	),(
R16402	T21350	T21349	arg2Of	(,.
R16403	T21351	T21349	arg3Of	A,.
R16404	T21353	T21349	arg1Of	in express,.
R16405	T21353	T21352	arg1Of	in express,) Prote
R16406	T21353	T21354	arg1Of	in express,io
R16407	T21353	T21361	arg1Of	in express,(da
R16408	T21353	T21362	arg2Of	in express,y 6) was
R16409	T21356	T21354	arg2Of	-cell ex,io
R1641	T1779	T1775	arg1Of	thymocytes,CD8+ single-positive
R16410	T21356	T21355	arg1Of	-cell ex,n in whole
R16411	T21356	T21357	arg1Of	-cell ex,t
R16412	T21358	T21357	arg2Of	rac,t
R16413	T21358	T21359	arg1Of	rac,t
R16414	T21360	T21357	arg3Of	s,t
R16415	T21362	T21335	arg3Of	y 6) was,was
R16416	T21362	T21361	arg2Of	y 6) was,(da
R16417	T21364	T21362	arg1Of	alyzed by immu,y 6) was
R16418	T21364	T21363	arg2Of	alyzed by immu,an
R16419	T21365	T21366	arg1Of	Overexpression,of
R1642	T1918	T1917	arg1Of	and,","
R16420	T21365	T21368	arg1Of	Overexpression,can
R16421	T21365	T21370	arg1Of	Overexpression,be
R16422	T21365	T21371	arg2Of	Overexpression,detected
R16423	T21367	T21366	arg2Of	Eomes-VP16,of
R16424	T21371	T21368	arg2Of	detected,can
R16425	T21371	T21369	arg1Of	detected,not
R16426	T21371	T21370	arg2Of	detected,be
R16427	T21371	T21372	arg1Of	detected,with
R16428	T21371	T21376	arg1Of	detected,","
R16429	T21371	T21377	arg1Of	detected,as
R1643	T1779	T1776	arg1Of	thymocytes,(
R16430	T21374	T21373	arg1Of	Eomes,the
R16431	T21375	T21372	arg2Of	antibody,with
R16432	T21375	T21374	arg1Of	antibody,Eomes
R16433	T21380	T21378	arg1Of	epitope,the
R16434	T21380	T21379	arg1Of	epitope,C-terminal
R16435	T21380	T21381	arg1Of	epitope,is
R16436	T21380	T21382	arg1Of	epitope,within
R16437	T21381	T21408	arg1Of	is,and
R16438	T21382	T21381	arg2Of	within,is
R16439	T21384	T21382	arg2Of	region,within
R1644	T1782	T1780	arg2Of	in,but
R16440	T21384	T21383	arg1Of	region,the
R16441	T21384	T21385	arg1Of	region,that
R16442	T21384	T21386	arg1Of	region,has
R16443	T21384	T21387	arg1Of	region,been
R16444	T21384	T21388	arg2Of	region,replaced
R16445	T21388	T21386	arg2Of	replaced,has
R16446	T21388	T21387	arg2Of	replaced,been
R16447	T21388	T21389	arg1Of	replaced,with
R16448	T21388	T21403	arg1Of	replaced,after
R16449	T21393	T21391	arg1Of	domain.,VP16
R1645	T1918	T1926	arg1Of	and,;
R16450	T21393	T21392	arg1Of	domain.,transactivation
R16451	T21393	T21394	arg1Of	domain.,(
R16452	T21395	T21394	arg2Of	B,(
R16453	T21396	T21394	arg3Of	),(
R16454	T21397	T21389	arg2Of	Expression,with
R16455	T21397	T21390	arg1Of	Expression,the
R16456	T21397	T21393	arg1Of	Expression,domain.
R16457	T21397	T21398	arg1Of	Expression,of
R16458	T21400	T21399	arg1Of	B,granzyme
R16459	T21400	T21401	arg1Of	B,and
R1646	T1920	T1921	arg1Of	Eomes,and
R16460	T21401	T21398	arg2Of	and,of
R16461	T21402	T21401	arg2Of	IFN-γ,and
R16462	T21404	T21403	arg2Of	culture,after
R16463	T21404	T21405	arg1Of	culture,for
R16464	T21407	T21405	arg2Of	d,for
R16465	T21407	T21406	arg1Of	d,6
R16466	T21408	T21377	arg2Of	and,as
R16467	T21409	T21410	arg1Of	restimulation,for
R16468	T21409	T21417	arg1Of	restimulation,was
R16469	T21409	T21418	arg2Of	restimulation,determined
R1647	T1921	T1919	arg1Of	and,both
R16470	T21412	T21410	arg2Of	h,for
R16471	T21412	T21411	arg1Of	h,4
R16472	T21412	T21413	arg1Of	h,with
R16473	T21414	T21415	arg1Of	PMA,and
R16474	T21415	T21413	arg2Of	and,with
R16475	T21416	T21415	arg2Of	ionomycin,and
R16476	T21418	T21408	arg2Of	determined,and
R16477	T21418	T21417	arg2Of	determined,was
R16478	T21421	T21418	arg1Of	staining,determined
R16479	T21421	T21419	arg2Of	staining,by
R1648	T1921	T1923	arg1Of	and,bind
R16480	T21421	T21420	arg1Of	staining,intracellular
R16481	T21423	T21422	arg1Of	percentage,The
R16482	T21423	T21424	arg1Of	percentage,of
R16483	T21423	T21428	arg1Of	percentage,is
R16484	T21423	T21429	arg2Of	percentage,shown
R16485	T21426	T21425	arg1Of	stained,positively
R16486	T21427	T21424	arg2Of	cells,of
R16487	T21427	T21426	arg1Of	cells,stained
R16488	T21429	T21428	arg2Of	shown,is
R16489	T21429	T21430	arg1Of	shown,above
R1649	T1922	T1921	arg2Of	Runx3,and
R16490	T21429	T21433	arg1Of	shown,;
R16491	T21432	T21430	arg2Of	gate,above
R16492	T21432	T21431	arg1Of	gate,the
R16493	T21437	T21434	arg1Of	intensity,the
R16494	T21437	T21435	arg1Of	intensity,mean
R16495	T21437	T21436	arg1Of	intensity,fluorescence
R16496	T21437	T21438	arg1Of	intensity,(
R16497	T21437	T21441	arg1Of	intensity,of
R16498	T21437	T21449	arg1Of	intensity,is
R16499	T21437	T21450	arg2Of	intensity,shown
R1650	T1923	T1918	arg2Of	bind,and
R16500	T21439	T21438	arg2Of	MFI,(
R16501	T21440	T21438	arg3Of	),(
R16502	T21444	T21441	arg2Of	staining,of
R16503	T21444	T21442	arg1Of	staining,granzyme
R16504	T21444	T21443	arg1Of	staining,B
R16505	T21444	T21445	arg1Of	staining,for
R16506	T21448	T21445	arg2Of	population,for
R16507	T21448	T21446	arg1Of	population,the
R16508	T21448	T21447	arg1Of	population,total
R16509	T21450	T21433	arg2Of	shown,;
R1651	T1923	T1924	arg1Of	bind,at
R16510	T21450	T21449	arg2Of	shown,is
R16511	T21450	T21451	arg1Of	shown,below
R16512	T21453	T21451	arg2Of	gate,below
R16513	T21453	T21452	arg1Of	gate,the
R16514	T21457	T21454	arg1Of	lines,The
R16515	T21457	T21455	arg1Of	lines,vertical
R16516	T21457	T21456	arg1Of	lines,gray
R16517	T21457	T21458	arg1Of	lines,indicate
R16518	T21460	T21458	arg2Of	MFI,indicate
R16519	T21460	T21459	arg1Of	MFI,the
R1652	T1925	T1924	arg2Of	the Prf1 locus,at
R16520	T21460	T21461	arg1Of	MFI,for
R16521	T21464	T21461	arg2Of	cells,for
R16522	T21464	T21462	arg1Of	cells,WT
R16523	T21464	T21463	arg1Of	cells,GFP+
R16524	T21465	T21466	arg1Of	Results,are
R16525	T21465	T21467	arg1Of	Results,representative
R16526	T21467	T21466	arg2Of	representative,are
R16527	T21467	T21468	arg1Of	representative,of
R16528	T21471	T21469	arg1Of	two,at
R16529	T21471	T21470	arg1Of	two,least
R1653	T1782	T1781	arg1Of	in,not
R16530	T21473	T21468	arg2Of	experiments.,of
R16531	T21473	T21471	arg1Of	experiments.,two
R16532	T21473	T21472	arg1Of	experiments.,independent
R16533	T21473	T21474	arg1Of	experiments.,(
R16534	T21473	T21478	arg1Of	experiments.,diagram
R16535	T21475	T21474	arg2Of	C,(
R16536	T21476	T21474	arg3Of	),(
R16537	T21478	T21477	arg1Of	diagram,Schematic
R16538	T21478	T21479	arg1Of	diagram,of
R16539	T21482	T21479	arg2Of	network,of
R1654	T1784	T1782	arg2Of	cells,in
R16540	T21482	T21480	arg1Of	network,the
R16541	T21482	T21481	arg1Of	network,transcriptional
R16542	T21482	T21483	arg1Of	network,involving
R16543	T21484	T21485	arg1Of	Runx3,and
R16544	T21485	T21483	arg2Of	and,involving
R16545	T21487	T21485	arg2Of	factors,and
R16546	T21487	T21486	arg1Of	factors,T-box
R16547	T21488	T21489	arg1Of	T-bet,is
R16548	T21488	T21490	arg2Of	T-bet,induced
R16549	T21488	T21495	arg1Of	T-bet,is
R1655	T1928	T1927	arg2Of	contrast,in
R16550	T21488	T21496	arg1Of	T-bet,essential
R16551	T21490	T21489	arg2Of	induced,is
R16552	T21490	T21494	arg1Of	induced,and
R16553	T21493	T21490	arg1Of	signals,induced
R16554	T21493	T21491	arg2Of	signals,by
R16555	T21493	T21492	arg1Of	signals,TCR
R16556	T21495	T21494	arg2Of	is,and
R16557	T21496	T21495	arg2Of	essential,is
R16558	T21496	T21497	arg1Of	essential,for
R16559	T21500	T21497	arg2Of	expression,for
R1656	T1784	T1783	arg1Of	cells,CD4+ SP
R16560	T21500	T21498	arg1Of	expression,early
R16561	T21500	T21499	arg1Of	expression,IFN-γ
R16562	T21501	T21502	arg1Of	Runx3,is
R16563	T21501	T21503	arg1Of	Runx3,present
R16564	T21501	T21510	arg1Of	Runx3,represses
R16565	T21501	T21513	arg1Of	Runx3,induces
R16566	T21502	T21509	arg1Of	is,and
R16567	T21503	T21502	arg2Of	present,is
R16568	T21503	T21504	arg1Of	present,in
R16569	T21508	T21504	arg2Of	cells,in
R1657	T1931	T1930	arg1Of	expression,perforin
R16570	T21508	T21505	arg1Of	cells,naive
R16571	T21508	T21506	arg1Of	cells,CD8+
R16572	T21508	T21507	arg1Of	cells,T
R16573	T21510	T21512	arg1Of	represses,and
R16574	T21511	T21510	arg2Of	Runx1,represses
R16575	T21512	T21509	arg2Of	and,and
R16576	T21513	T21512	arg2Of	induces,and
R16577	T21514	T21513	arg2Of	Eomes,induces
R16578	T21514	T21515	arg1Of	Eomes,","
R16579	T21516	T21517	arg1Of	perforin,","
R1658	T1931	T1932	arg1Of	expression,is
R16580	T21517	T21521	arg1Of	",",and
R16581	T21519	T21517	arg2Of	B,","
R16582	T21519	T21518	arg1Of	B,granzyme
R16583	T21521	T21515	arg2Of	and,","
R16584	T21521	T21520	arg1Of	and,","
R16585	T21523	T21521	arg2Of	expression,and
R16586	T21523	T21522	arg1Of	expression,IFN-γ
R16587	T21524	T21525	arg1Of	Eomes,may
R16588	T21524	T21526	arg1Of	Eomes,participate
R16589	T21526	T21525	arg2Of	participate,may
R1659	T1931	T1933	arg1Of	expression,unaffected
R16590	T21526	T21527	arg1Of	participate,in
R16591	T21526	T21533	arg1Of	participate,whereas
R16592	T21528	T21527	arg2Of	sustaining,in
R16593	T21531	T21528	arg2Of	expression,sustaining
R16594	T21531	T21529	arg1Of	expression,late
R16595	T21531	T21530	arg1Of	expression,IFN-γ
R16596	T21533	T21532	arg1Of	whereas,","
R16597	T21534	T21535	arg1Of	Runx3,and
R16598	T21535	T21537	arg1Of	and,(
R16599	T21535	T21542	arg1Of	and,may
R1660	T1784	T1785	arg1Of	cells,(
R16600	T21535	T21543	arg1Of	and,cooperate
R16601	T21535	T21545	arg1Of	and,activate
R16602	T21536	T21535	arg2Of	Eomes,and
R16603	T21538	T21539	arg1Of	but,not
R16604	T21540	T21537	arg2Of	T-bet,(
R16605	T21540	T21538	arg1Of	T-bet,but
R16606	T21541	T21537	arg3Of	),(
R16607	T21543	T21533	arg2Of	cooperate,whereas
R16608	T21543	T21542	arg2Of	cooperate,may
R16609	T21545	T21543	arg2Of	activate,cooperate
R1661	T1932	T1926	arg2Of	is,;
R16610	T21545	T21544	arg1Of	activate,to
R16611	T21547	T21545	arg2Of	expression,activate
R16612	T21547	T21546	arg1Of	expression,perforin
R16613	T21550	T21548	arg1Of	line,The
R16614	T21550	T21549	arg1Of	line,dotted
R16615	T21550	T21551	arg1Of	line,indicates
R16616	T21554	T21551	arg2Of	effect,indicates
R16617	T21554	T21552	arg1Of	effect,the
R16618	T21554	T21553	arg1Of	effect,partial
R16619	T21554	T21555	arg1Of	effect,of
R1662	T1932	T1927	arg1Of	is,in
R16620	T21554	T21558	arg1Of	effect,on
R16621	T21557	T21555	arg2Of	deficiency,of
R16622	T21557	T21556	arg1Of	deficiency,T-bet
R16623	T21560	T21559	arg1Of	mRNA,Gzmb
R16624	T21560	T21561	arg1Of	mRNA,but
R16625	T21561	T21558	arg2Of	but,on
R16626	T21566	T21561	arg2Of	expression,but
R16627	T21566	T21562	arg1Of	expression,not
R16628	T21566	T21563	arg1Of	expression,granzyme
R16629	T21566	T21564	arg1Of	expression,B
R1663	T1932	T1929	arg1Of	is,","
R16630	T21566	T21565	arg1Of	expression,protein
R1664	T1932	T1934	arg1Of	is,in
R1665	T1933	T1932	arg2Of	unaffected,is
R1666	T1786	T1785	arg2Of	14,(
R1667	T1936	T1934	arg2Of	cells,in
R1668	T1787	T1785	arg3Of	),(
R1669	T1936	T1935	arg1Of	cells,T-bet–deficient
R1670	T1938	T1937	arg1Of	cells,T
R1671	T1938	T1939	arg1Of	cells,lacking
R1672	T1938	T1953	arg1Of	cells,binds
R1673	T1789	T1790	arg1Of	Runx3,is
R1674	T1941	T1939	arg2Of	show,lacking
R1675	T1941	T1940	arg1Of	show,Runx3
R1676	T1941	T1942	arg2Of	show,decreased
R1677	T1943	T1942	arg3Of	expression,decreased
R1678	T1943	T1944	arg1Of	expression,of
R1679	T1789	T1792	arg2Of	Runx3,expressed
R1680	T1945	T1946	arg1Of	IFN-γ,and
R1681	T1792	T1788	arg2Of	expressed,Although
R1682	T1946	T1950	arg1Of	and,and
R1683	T1948	T1946	arg2Of	B,and
R1684	T1948	T1947	arg1Of	B,granzyme
R1685	T1950	T1944	arg2Of	and,of
R1686	T1950	T1949	arg1Of	and,","
R1687	T1951	T1950	arg2Of	Runx3,and
R1688	T1792	T1790	arg2Of	expressed,is
R1689	T1953	T1952	arg1Of	binds,also
R1690	T1792	T1791	arg1Of	expressed,not
R1691	T1792	T1793	arg1Of	expressed,in
R1692	T1796	T1793	arg2Of	cells,in
R1693	T1956	T1953	arg2Of	regions,binds
R1694	T1956	T1954	arg1Of	regions,the
R1695	T1956	T1955	arg1Of	regions,promoter
R1696	T1956	T1957	arg1Of	regions,of
R1697	T1958	T1957	arg2Of	the Ifng and Gzmb genes,of
R1698	T1962	T1961	arg1Of	results,these
R1699	T1962	T1963	arg1Of	results,provide
R1700	T1963	T1959	arg1Of	provide,Collectively
R1701	T1963	T1960	arg1Of	provide,","
R1702	T1964	T1963	arg2Of	evidence,provide
R1703	T1964	T1965	arg1Of	evidence,for
R1704	T1796	T1794	arg1Of	cells,naive
R1705	T1796	T1795	arg1Of	cells,CD4+ T
R1707	T1969	T1965	arg2Of	network,for
R1708	T1969	T1966	arg1Of	network,a
R1709	T1969	T1967	arg1Of	network,complex
R1710	T1969	T1968	arg1Of	network,transcriptional
R1711	T1969	T1970	arg2Of	network,in
R1712	T1969	T1971	arg1Of	network,which
R1713	T1972	T1973	arg1Of	Runx3,is
R1714	T1972	T1979	arg1Of	Runx3,synergizes
R1715	T1973	T1978	arg1Of	is,but
R1718	T1977	T1973	arg2Of	of Gzmb expression,is
R1719	T1977	T1974	arg1Of	of Gzmb expression,a
R1720	T1977	T1975	arg1Of	of Gzmb expression,primary
R1721	T1977	T1976	arg1Of	of Gzmb expression,regulator
R1722	T1978	T1970	arg1Of	but,in
R1723	T1978	T1986	arg1Of	but,","
R1724	T1978	T1987	modOf	but,to
R1726	T1979	T1978	arg2Of	synergizes,but
R1727	T1979	T1980	arg1Of	synergizes,with
R1728	T1979	T1984	arg1Of	synergizes,","
R1729	T1979	T1985	arg1Of	synergizes,respectively
R1731	T1981	T1982	arg1Of	T-bet,and
R1732	T1982	T1980	arg2Of	and,with
R1733	T1983	T1982	arg2Of	Eomes,and
R1735	T1988	T1987	arg1Of	promote,to
R1736	T1989	T1988	arg2Of	transcription,promote
R1737	T1989	T1990	arg1Of	transcription,of
R1738	T1991	T1990	arg2Of	the Ifng and Prf1 genes,of
R201	T270	T271	arg1Of	Runx3,and
R202	T272	T271	arg2Of	T-box,and
R203	T273	T270	arg1Of	proteins,Runx3
R204	T273	T272	arg1Of	proteins,T-box
R205	T273	T274	arg1Of	proteins,cooperate
R206	T273	T276	arg1Of	proteins,establish
R207	T276	T274	arg2Of	establish,cooperate
R208	T276	T275	arg1Of	establish,to
R209	T279	T276	arg2Of	program,establish
R210	T279	T277	arg1Of	program,the
R211	T279	T278	arg1Of	program,transcriptional
R212	T279	T280	arg1Of	program,of
R213	T282	T280	arg2Of	CTLs,of
R214	T282	T281	arg1Of	CTLs,effector
R215	T283	T284	arg1Of	Activation,of
R216	T283	T289	arg1Of	Activation,with
R217	T283	T291	arg1Of	Activation,induces
R218	T288	T284	arg2Of	cells,of
R219	T288	T285	arg1Of	cells,naive
R220	T288	T286	arg1Of	cells,CD8+
R221	T288	T287	arg1Of	cells,T
R222	T290	T289	arg2Of	antigen,with
R223	T293	T291	arg2Of	differentiation,induces
R224	T293	T292	arg1Of	differentiation,their
R225	T293	T294	arg1Of	differentiation,into
R226	T298	T294	arg2Of	lymphocytes,into
R227	T298	T295	arg1Of	lymphocytes,effector
R228	T298	T296	arg1Of	lymphocytes,cytolytic
R2286	T2916	T2913	arg1Of	system,A
R2287	T2916	T2914	arg1Of	system,cell
R2288	T2916	T2915	arg1Of	system,culture
R2289	T2916	T2917	modOf	system,to
R229	T298	T297	arg1Of	lymphocytes,T
R2290	T2918	T2917	arg1Of	monitor,to
R2291	T2921	T2918	arg2Of	differentiation,monitor
R2292	T2921	T2919	arg1Of	differentiation,effector
R2293	T2921	T2920	arg1Of	differentiation,CTL
R2294	T2922	T2923	arg1Of	We,used
R2295	T2922	T2930	arg1Of	We,examine
R2296	T2928	T2923	arg2Of	system,used
R2297	T2928	T2924	arg1Of	system,a
R2298	T2928	T2925	arg1Of	system,simple
R2299	T2928	T2926	arg1Of	system,cell
R230	T298	T299	arg1Of	lymphocytes,(
R2300	T2928	T2927	arg1Of	system,culture
R2301	T2930	T2923	arg3Of	examine,used
R2302	T2930	T2929	arg1Of	examine,to
R2303	T2932	T2930	arg2Of	kinetics,examine
R2304	T2932	T2931	arg1Of	kinetics,the
R2305	T2932	T2933	arg1Of	kinetics,of
R2306	T2932	T2937	arg1Of	kinetics,during
R2307	T2936	T2933	arg2Of	expression,of
R2308	T2936	T2934	arg1Of	expression,effector
R2309	T2936	T2935	arg1Of	expression,gene
R231	T300	T299	arg2Of	CTLs,(
R2310	T2941	T2937	arg2Of	differentiation,during
R2311	T2941	T2938	arg1Of	differentiation,CD8+
R2312	T2941	T2939	arg1Of	differentiation,T
R2313	T2941	T2940	arg1Of	differentiation,cell
R2314	T2945	T2942	arg1Of	cells,Naive
R2315	T2945	T2943	arg1Of	cells,CD8+
R2316	T2945	T2944	arg1Of	cells,T
R2317	T2945	T2946	arg1Of	cells,from
R2318	T2945	T2951	arg1Of	cells,were
R2319	T2945	T2952	arg2Of	cells,activated
R232	T301	T299	arg3Of	),(
R2320	T2945	T2972	arg1Of	cells,were
R2321	T2945	T2973	arg2Of	cells,cultured
R2322	T2950	T2946	arg2Of	mice,from
R2323	T2950	T2947	arg1Of	mice,P14
R2324	T2950	T2948	arg1Of	mice,TCR
R2325	T2950	T2949	arg1Of	mice,transgenic
R2326	T2952	T2951	arg2Of	activated,were
R2327	T2952	T2953	arg1Of	activated,for
R2328	T2952	T2956	arg1Of	activated,with
R2329	T2952	T2961	arg1Of	activated,with
R233	T302	T303	arg1Of	CTLs,lyse
R2330	T2952	T2964	arg1Of	activated,in
R2331	T2952	T2971	arg1Of	activated,and
R2332	T2955	T2953	arg2Of	d,for
R2333	T2955	T2954	arg1Of	d,2
R2334	T2956	T2960	arg1Of	with,or
R2335	T2957	T2958	arg1Of	anti-CD3,and
R2336	T2958	T2956	arg2Of	and,with
R2337	T2959	T2958	arg2Of	anti-CD28,and
R2338	T2961	T2960	arg2Of	with,or
R2339	T2963	T2961	arg2Of	APCs,with
R234	T303	T309	arg1Of	lyse,by
R2340	T2963	T2962	arg1Of	APCs,splenic
R2341	T2966	T2964	arg2Of	presence,in
R2342	T2966	T2965	arg1Of	presence,the
R2343	T2966	T2967	arg1Of	presence,of
R2344	T2969	T2967	arg2Of	peptide,of
R2345	T2969	T2968	arg1Of	peptide,Gp33
R2346	T2971	T2970	arg1Of	and,","
R2347	T2973	T2971	arg2Of	cultured,and
R2348	T2973	T2972	arg2Of	cultured,were
R2349	T2973	T2974	arg1Of	cultured,in
R235	T304	T305	arg1Of	infected,or
R2350	T2975	T2974	arg2Of	media,in
R2351	T2975	T2976	arg1Of	media,containing
R2352	T2978	T2976	arg2Of	U/ml,containing
R2353	T2978	T2977	arg1Of	U/ml,100
R2354	T2978	T2979	arg1Of	U/ml,of
R2355	T2982	T2979	arg2Of	IL-2,of
R2356	T2982	T2980	arg1Of	IL-2,recombinant
R2357	T2982	T2981	arg1Of	IL-2,human
R2358	T2982	T2983	arg1Of	IL-2,(
R2359	T2984	T2983	arg2Of	rhIL-2,(
R236	T306	T305	arg2Of	aberrant,or
R2360	T2985	T2983	arg3Of	),(
R2361	T2986	T2987	arg1Of	We,used
R2362	T2987	T2994	arg1Of	used,because
R2363	T2987	T3008	arg1Of	used,;
R2364	T2990	T2987	arg2Of	mice,used
R2365	T2990	T2988	arg1Of	mice,TCR
R2366	T2990	T2989	arg1Of	mice,transgenic
R2367	T2990	T2991	arg1Of	mice,for
R2368	T2993	T2991	arg2Of	experiments,for
R2369	T2993	T2992	arg1Of	experiments,these
R237	T308	T303	arg2Of	cells,lyse
R2370	T2995	T2996	arg1Of	they,provide
R2371	T2996	T2994	arg2Of	provide,because
R2372	T2999	T2996	arg2Of	source,provide
R2373	T2999	T2997	arg1Of	source,a
R2374	T2999	T2998	arg1Of	source,reliable
R2375	T2999	T3000	arg1Of	source,of
R2376	T3003	T3000	arg2Of	cells,of
R2377	T3003	T3001	arg1Of	cells,CD8+
R2378	T3003	T3002	arg1Of	cells,T
R2379	T3003	T3004	arg1Of	cells,that
R238	T308	T304	arg1Of	cells,infected
R2380	T3003	T3005	arg1Of	cells,are
R2381	T3003	T3007	arg1Of	cells,naive
R2382	T3007	T3005	arg2Of	naive,are
R2383	T3007	T3006	arg1Of	naive,truly
R2384	T3008	T3009	arg1Of	;,however
R2385	T3011	T3012	arg1Of	we,chose
R2386	T3011	T3015	arg1Of	we,stimulate
R2387	T3012	T3008	arg2Of	chose,;
R2388	T3012	T3010	arg1Of	chose,","
R2389	T3012	T3023	arg1Of	chose,as
R239	T308	T306	arg1Of	cells,aberrant
R2390	T3015	T3012	arg2Of	stimulate,chose
R2391	T3015	T3013	arg1Of	stimulate,not
R2392	T3015	T3014	arg1Of	stimulate,to
R2393	T3015	T3019	arg1Of	stimulate,in
R2394	T3016	T3015	arg2Of	cells,stimulate
R2395	T3016	T3017	arg1Of	cells,with
R2396	T3018	T3017	arg2Of	antigen,with
R2397	T3021	T3019	arg2Of	experiments,in
R2398	T3021	T3020	arg1Of	experiments,most
R2399	T3023	T3022	arg1Of	as,so
R240	T308	T307	arg1Of	cells,target
R2400	T3025	T3023	arg2Of	avoid,as
R2401	T3025	T3024	arg1Of	avoid,to
R2402	T3026	T3025	arg2Of	contamination,avoid
R2403	T3026	T3027	arg1Of	contamination,with
R2404	T3028	T3029	arg1Of	proteins,and
R2405	T3029	T3027	arg2Of	and,with
R2406	T3029	T3032	arg2Of	and,derived
R2407	T3031	T3029	arg2Of	acids,and
R2408	T3031	T3030	arg1Of	acids,nucleic
R2409	T3032	T3033	arg1Of	derived,from
R241	T310	T309	arg2Of	exocytosis,by
R2410	T3034	T3033	arg2Of	APCs,from
R2411	T3035	T3036	arg1Of	There,were
R2412	T3036	T3043	arg1Of	were,during
R2413	T3036	T3051	arg1Of	were,and
R2414	T3038	T3037	arg1Of	minor,only
R2415	T3039	T3036	arg2Of	differences,were
R2416	T3039	T3038	arg1Of	differences,minor
R2417	T3039	T3040	arg1Of	differences,in
R2418	T3042	T3040	arg2Of	expression,in
R2419	T3042	T3041	arg1Of	expression,gene
R242	T310	T311	arg1Of	exocytosis,of
R2420	T3044	T3043	arg2Of	differentiation,during
R2421	T3044	T3045	arg2Of	differentiation,induced
R2422	T3047	T3045	arg1Of	antigen/APC,induced
R2423	T3047	T3046	arg2Of	antigen/APC,by
R2424	T3047	T3048	arg1Of	antigen/APC,versus
R2425	T3049	T3048	arg2Of	anti-CD3/anti-CD28,versus
R2426	T3051	T3050	arg1Of	and,","
R2427	T3054	T3052	arg1Of	conclusions,the
R2428	T3054	T3053	arg1Of	conclusions,major
R2429	T3054	T3055	arg2Of	conclusions,presented
R243	T313	T311	arg2Of	granules,of
R2430	T3054	T3059	arg1Of	conclusions,are
R2431	T3055	T3056	arg1Of	presented,in
R2432	T3058	T3056	arg2Of	report,in
R2433	T3058	T3057	arg1Of	report,this
R2434	T3059	T3051	arg2Of	are,and
R2435	T3059	T3062	arg1Of	are,for
R2436	T3061	T3059	arg2Of	same,are
R2437	T3061	T3060	arg1Of	same,the
R2438	T3065	T3062	arg2Of	conditions,for
R2439	T3065	T3063	arg1Of	conditions,both
R244	T313	T312	arg1Of	granules,lytic
R2440	T3065	T3064	arg1Of	conditions,activating
R2441	T3069	T3066	arg2Of	conditions,Under
R2442	T3069	T3067	arg1Of	conditions,our
R2443	T3069	T3068	arg1Of	conditions,culture
R2444	T3074	T3071	arg2Of	cells,activated
R2445	T3074	T3072	arg1Of	cells,CD8+
R2446	T3074	T3073	arg1Of	cells,T
R2447	T3074	T3075	arg1Of	cells,expanded
R2448	T3074	T3078	arg2Of	cells,accumulated
R2449	T3075	T3076	arg1Of	expanded,exponentially
R245	T313	T314	arg1Of	granules,containing
R2450	T3075	T3077	arg1Of	expanded,and
R2451	T3077	T3066	arg1Of	and,Under
R2452	T3077	T3070	arg1Of	and,","
R2453	T3078	T3077	arg2Of	accumulated,and
R2454	T3078	T3079	arg1Of	accumulated,for
R2455	T3081	T3080	arg1Of	8,>
R2456	T3082	T3079	arg2Of	d,for
R2457	T3082	T3081	arg1Of	d,8
R2458	T3083	T3084	arg1Of	We,limited
R2459	T3084	T3087	arg1Of	limited,to
R246	T318	T315	arg1Of	perforin,the
R2460	T3084	T3092	arg1Of	limited,after
R2461	T3086	T3084	arg2Of	analysis,limited
R2462	T3086	T3085	arg1Of	analysis,our
R2463	T3091	T3087	arg2Of	d,to
R2464	T3091	T3088	arg1Of	d,the
R2465	T3091	T3089	arg1Of	d,first
R2466	T3091	T3090	arg1Of	d,6–8
R2467	T3093	T3092	arg2Of	activation,after
R2468	T3093	T3094	arg1Of	activation,","
R2469	T3096	T3094	arg2Of	period,","
R247	T318	T316	arg1Of	perforin,pore-forming
R2470	T3096	T3095	arg1Of	period,a
R2471	T3096	T3097	arg1Of	period,that
R2472	T3096	T3098	arg1Of	period,coincides
R2473	T3098	T3099	arg1Of	coincides,with
R2474	T3098	T3106	arg1Of	coincides,after
R2475	T3101	T3099	arg2Of	expansion,with
R2476	T3101	T3100	arg1Of	expansion,clonal
R2477	T3101	T3102	arg1Of	expansion,of
R2478	T3105	T3102	arg2Of	cells,of
R2479	T3105	T3103	arg1Of	cells,CD8+
R248	T318	T317	arg1Of	perforin,protein
R2480	T3105	T3104	arg1Of	cells,T
R2481	T3107	T3106	arg2Of	activation,after
R2482	T3107	T3109	arg1Of	activation,vivo
R2483	T3109	T3108	arg1Of	vivo,in
R249	T318	T319	arg1Of	perforin,and
R250	T319	T314	arg2Of	and,containing
R251	T321	T319	arg2Of	family,and
R252	T321	T320	arg1Of	family,a
R253	T321	T322	arg1Of	family,of
R254	T323	T322	arg2Of	proteases,of
R255	T323	T324	arg2Of	proteases,termed
R256	T325	T324	arg3Of	granzymes,termed
R257	T326	T327	arg1Of	We,show
R258	T331	T329	arg1Of	differentiation,effector
R259	T331	T330	arg1Of	differentiation,CTL
R260	T331	T332	arg1Of	differentiation,occurs
R261	T332	T327	arg2Of	occurs,show
R262	T332	T328	arg1Of	occurs,that
R263	T332	T333	arg1Of	occurs,in
R264	T336	T334	arg1Of	phases,two
R265	T336	T335	arg1Of	phases,sequential
R266	T336	T338	arg1Of	phases,vitro
R267	T336	T339	arg1Of	phases,","
R268	T336	T340	arg2Of	phases,characterized
R269	T336	T354	arg1Of	phases,","
R270	T338	T337	arg1Of	vitro,in
R271	T343	T340	arg1Of	induction,characterized
R272	T343	T341	arg2Of	induction,by
R273	T343	T342	arg1Of	induction,early
R274	T343	T344	arg1Of	induction,of
R275	T345	T346	arg1Of	T-bet,and
R276	T347	T346	arg2Of	late,and
R277	T348	T344	arg2Of	induction,of
R278	T348	T345	arg1Of	induction,T-bet
R279	T348	T347	arg1Of	induction,late
R280	T348	T349	arg1Of	induction,of
R281	T350	T349	arg2Of	Eomesodermin,of
R282	T350	T351	arg1Of	Eomesodermin,(
R283	T352	T351	arg2Of	Eomes,(
R284	T353	T351	arg3Of	),(
R285	T354	T333	arg2Of	",",in
R286	T354	T358	arg1Of	",",that
R287	T354	T359	arg1Of	",",regulate
R288	T357	T354	arg2Of	factors,","
R289	T357	T355	arg1Of	factors,T-box
R290	T357	T356	arg1Of	factors,transcription
R291	T359	T372	arg1Of	regulate,","
R292	T359	T373	arg1Of	regulate,respectively
R293	T361	T362	arg1Of	early,and
R294	T363	T362	arg2Of	late,and
R295	T364	T359	arg2Of	phases,regulate
R296	T364	T360	arg1Of	phases,the
R297	T364	T361	arg1Of	phases,early
R298	T364	T363	arg1Of	phases,late
R299	T364	T365	arg1Of	phases,of
R300	T368	T367	arg2Of	IFN,(
R3009	T3827	T3825	arg1Of	kinetics,Distinct
R301	T369	T367	arg3Of	),(
R3010	T3827	T3826	arg1Of	kinetics,expression
R3011	T3827	T3828	arg1Of	kinetics,of
R3012	T3829	T3830	arg1Of	perforin,and
R3013	T3830	T3828	arg2Of	and,of
R3014	T3830	T3833	arg1Of	and,during
R3015	T3832	T3830	arg2Of	B,and
R3016	T3832	T3831	arg1Of	B,granzyme
R3017	T3835	T3833	arg2Of	development,during
R3018	T3835	T3834	arg1Of	development,CTL
R3019	T3835	T3836	arg1Of	development,in
R302	T371	T365	arg2Of	expression,of
R3020	T3837	T3836	arg2Of	culture,in
R3021	T3839	T3838	arg1Of	experiments,Our
R3022	T3839	T3840	arg1Of	experiments,revealed
R3023	T3842	T3840	arg2Of	differences,revealed
R3024	T3842	T3841	arg1Of	differences,clear
R3025	T3842	T3843	arg1Of	differences,in
R3026	T3842	T3855	arg1Of	differences,during
R3027	T3845	T3843	arg2Of	kinetics,in
R3028	T3845	T3844	arg1Of	kinetics,the
R3029	T3845	T3846	arg1Of	kinetics,of
R303	T371	T366	arg1Of	expression,interferon
R3030	T3847	T3848	arg1Of	perforin,","
R3031	T3848	T3852	arg1Of	",",and
R3032	T3850	T3848	arg2Of	B,","
R3033	T3850	T3849	arg1Of	B,granzyme
R3034	T3852	T3846	arg2Of	and,of
R3035	T3852	T3851	arg1Of	and,","
R3036	T3854	T3852	arg2Of	expression,and
R3037	T3854	T3853	arg1Of	expression,cytokine
R3038	T3859	T3855	arg2Of	activation,during
R3039	T3859	T3856	arg1Of	activation,CD8+
R304	T371	T367	arg1Of	expression,(
R3040	T3859	T3857	arg1Of	activation,T
R3041	T3859	T3858	arg1Of	activation,cell
R3042	T3859	T3860	arg1Of	activation,(
R3043	T3861	T3860	arg2Of	Fig.,(
R3044	T3861	T3862	arg1Of	Fig.,1
R3045	T3863	T3860	arg3Of	),(
R3046	T3866	T3864	arg1Of	cells,Naive
R3047	T3866	T3865	arg1Of	cells,T
R3048	T3866	T3867	arg1Of	cells,showed
R3049	T3869	T3868	arg1Of	expression,detectable
R305	T371	T370	arg1Of	expression,γ
R3050	T3869	T3870	arg1Of	expression,of
R3051	T3869	T3875	arg1Of	expression,as
R3052	T3872	T3870	arg2Of	mRNA,of
R3053	T3872	T3871	arg1Of	mRNA,perforin
R3054	T3875	T3867	arg2Of	as,showed
R3055	T3875	T3873	arg1Of	as,as
R3056	T3875	T3874	arg1Of	as,well
R3057	T3877	T3875	arg2Of	protein,as
R3058	T3877	T3876	arg1Of	protein,perforin
R3059	T3877	T3878	arg1Of	protein,(
R306	T375	T374	arg2Of	addition,In
R3060	T3879	T3878	arg2Of	Fig.,(
R3061	T3879	T3880	arg1Of	Fig.,1
R3062	T3879	T3881	arg1Of	Fig.,","
R3063	T3882	T3881	arg2Of	A–D,","
R3064	T3883	T3878	arg3Of	),(
R3065	T3885	T3884	arg1Of	to,Relative
R3066	T3887	T3885	arg2Of	expression,to
R3067	T3887	T3886	arg1Of	expression,its
R3068	T3887	T3888	arg1Of	expression,in
R3069	T3891	T3888	arg2Of	cells,in
R307	T377	T378	arg1Of	we,demonstrate
R3070	T3891	T3889	arg1Of	cells,naive
R3071	T3891	T3890	arg1Of	cells,T
R3072	T3895	T3894	arg2Of	Prf1,(
R3073	T3896	T3894	arg3Of	),(
R3074	T3898	T3893	arg1Of	expression,perforin
R3075	T3898	T3894	arg1Of	expression,(
R3076	T3898	T3897	arg1Of	expression,mRNA
R3077	T3898	T3899	arg1Of	expression,did
R3078	T3898	T3901	arg1Of	expression,increase
R3079	T3898	T3907	arg1Of	expression,showed
R308	T378	T374	arg1Of	demonstrate,In
R3080	T3901	T3899	arg2Of	increase,did
R3081	T3901	T3900	arg1Of	increase,not
R3082	T3901	T3902	arg1Of	increase,appreciably
R3083	T3901	T3903	arg1Of	increase,at
R3084	T3901	T3906	arg1Of	increase,but
R3085	T3904	T3903	arg2Of	day,at
R3086	T3904	T3905	arg1Of	day,2
R3087	T3906	T3885	arg1Of	but,to
R3088	T3906	T3892	arg1Of	but,","
R3089	T3906	T3914	arg1Of	but,","
R309	T378	T376	arg1Of	demonstrate,","
R3090	T3906	T3915	modOf	but,followed
R3091	T3907	T3906	arg2Of	showed,but
R3092	T3910	T3907	arg2Of	decrease,showed
R3093	T3910	T3908	arg1Of	decrease,a
R3094	T3910	T3909	arg1Of	decrease,reproducible
R3095	T3910	T3911	arg1Of	decrease,at
R3096	T3912	T3911	arg2Of	day,at
R3097	T3912	T3913	arg1Of	day,4
R3098	T3918	T3915	arg1Of	reexpression,followed
R3099	T3918	T3916	arg2Of	reexpression,by
R310	T381	T378	arg2Of	role,demonstrate
R3100	T3918	T3917	arg1Of	reexpression,robust
R3101	T3918	T3919	arg1Of	reexpression,between
R3102	T3921	T3922	arg1Of	4,and
R3103	T3922	T3919	arg2Of	and,between
R3104	T3922	T3920	arg1Of	and,days
R3105	T3922	T3924	arg1Of	and,(
R3106	T3923	T3922	arg2Of	8,and
R3107	T3925	T3924	arg2Of	Fig.,(
R3108	T3925	T3926	arg1Of	Fig.,1
R3109	T3925	T3927	arg1Of	Fig.,","
R311	T381	T379	arg1Of	role,a
R3110	T3928	T3927	arg2Of	A–D,","
R3111	T3929	T3924	arg3Of	),(
R3112	T3931	T3930	arg2Of	contrast,In
R3113	T3936	T3935	arg2Of	Gzmb,(
R3114	T3937	T3935	arg3Of	),(
R3115	T3938	T3933	arg1Of	mRNA,granzyme
R3116	T3938	T3934	arg1Of	mRNA,B
R3117	T3938	T3935	arg1Of	mRNA,(
R3118	T3938	T3939	arg1Of	mRNA,was
R3119	T3938	T3940	arg1Of	mRNA,low
R312	T381	T380	arg1Of	role,critical
R3120	T3938	T3942	arg1Of	mRNA,undetectable
R3121	T3938	T3948	arg1Of	mRNA,was
R3122	T3938	T3950	arg2Of	mRNA,up-regulated
R3123	T3938	T3957	arg2Of	mRNA,increased
R3124	T3939	T3947	arg1Of	was,but
R3125	T3940	T3941	arg1Of	low,or
R3126	T3941	T3939	arg2Of	or,was
R3127	T3941	T3943	arg1Of	or,in
R3128	T3942	T3941	arg2Of	undetectable,or
R3129	T3946	T3943	arg2Of	cells,in
R313	T381	T382	arg1Of	role,for
R3130	T3946	T3944	arg1Of	cells,naive
R3131	T3946	T3945	arg1Of	cells,T
R3132	T3947	T3930	arg1Of	but,In
R3133	T3947	T3932	arg1Of	but,","
R3134	T3950	T3951	arg1Of	up-regulated,by
R3135	T3950	T3956	arg1Of	up-regulated,and
R3136	T3952	T3951	arg2Of	day,by
R3137	T3952	T3953	arg1Of	day,2
R3138	T3952	T3954	arg1Of	day,after
R3139	T3955	T3954	arg2Of	stimulation,after
R314	T381	T387	arg1Of	role,in
R3140	T3956	T3947	arg2Of	and,but
R3141	T3956	T3948	arg2Of	and,was
R3142	T3956	T3949	arg1Of	and,strongly
R3143	T3957	T3956	arg2Of	increased,and
R3144	T3957	T3958	arg1Of	increased,progressively
R3145	T3957	T3959	arg1Of	increased,until
R3146	T3957	T3970	arg1Of	increased,;
R3147	T3960	T3959	arg2Of	day,until
R3148	T3960	T3961	arg1Of	day,6
R3149	T3960	T3962	arg1Of	day,(
R315	T386	T382	arg2Of	Runx3,for
R3150	T3963	T3962	arg2Of	Fig.,(
R3151	T3963	T3964	arg1Of	Fig.,1
R3152	T3963	T3965	arg1Of	Fig.,","
R3153	T3966	T3967	arg1Of	A,and
R3154	T3967	T3965	arg2Of	and,","
R3155	T3968	T3967	arg2Of	B,and
R3156	T3969	T3962	arg3Of	),(
R3157	T3975	T3973	arg1Of	protein,granzyme
R3158	T3975	T3974	arg1Of	protein,B
R3159	T3975	T3976	arg1Of	protein,was
R316	T386	T383	arg1Of	Runx3,the
R3160	T3975	T3977	arg2Of	protein,expressed
R3161	T3975	T3982	arg1Of	protein,remained
R3162	T3975	T3983	arg1Of	protein,high
R3163	T3977	T3976	arg2Of	expressed,was
R3164	T3977	T3978	arg1Of	expressed,by
R3165	T3977	T3981	arg1Of	expressed,and
R3166	T3979	T3978	arg2Of	day,by
R3167	T3979	T3980	arg1Of	day,4
R3168	T3981	T3970	arg2Of	and,;
R3169	T3981	T3971	arg1Of	and,similarly
R317	T386	T384	arg1Of	Runx3,transcription
R3170	T3981	T3972	arg1Of	and,","
R3171	T3982	T3981	arg2Of	remained,and
R3172	T3983	T3982	arg2Of	high,remained
R3173	T3983	T3984	arg1Of	high,until
R3174	T3985	T3984	arg2Of	day,until
R3175	T3985	T3986	arg1Of	day,6
R3176	T3985	T3987	arg1Of	day,(
R3177	T3990	T3987	arg2Of	E,(
R3178	T3990	T3988	arg1Of	E,Fig.
R3179	T3990	T3989	arg1Of	E,1
R318	T386	T385	arg1Of	Runx3,factor
R3180	T3991	T3987	arg3Of	),(
R3181	T3993	T3992	arg2Of	expected,As
R3182	T3997	T3995	arg1Of	fraction,a
R3183	T3997	T3996	arg1Of	fraction,small
R3184	T3997	T3998	arg1Of	fraction,of
R3185	T3997	T4002	arg1Of	fraction,expressed
R3186	T4001	T3998	arg2Of	cells,of
R3187	T4001	T3999	arg1Of	cells,naive
R3188	T4001	T4000	arg1Of	cells,T
R3189	T4002	T4008	arg1Of	expressed,in
R319	T389	T387	arg2Of	differentiation,in
R3190	T4002	T4013	arg1Of	expressed,and
R3191	T4005	T4006	arg1Of	IFN-γ,and
R3192	T4006	T4002	arg2Of	and,expressed
R3193	T4006	T4003	arg1Of	and,the
R3194	T4006	T4004	arg1Of	and,cytokines
R3195	T4007	T4006	arg2Of	TNF,and
R3196	T4008	T4010	arg1Of	in,to
R3197	T4009	T4008	arg2Of	response,in
R3198	T4011	T4008	arg3Of	stimulation,in
R3199	T4013	T3992	arg1Of	and,As
R320	T389	T388	arg1Of	differentiation,CTL
R3200	T4013	T3994	arg1Of	and,","
R3201	T4013	T4012	arg1Of	and,","
R3202	T4015	T4014	arg1Of	capacity,this
R3203	T4015	T4016	arg1Of	capacity,increased
R3204	T4016	T4013	arg2Of	increased,and
R3205	T4016	T4017	arg1Of	increased,significantly
R3206	T4016	T4018	arg1Of	increased,in
R3207	T4016	T4021	arg1Of	increased,(
R3208	T4020	T4018	arg2Of	cells,in
R3209	T4020	T4019	arg2Of	cells,differentiated
R321	T390	T391	arg1Of	Runx3,regulates
R3210	T4024	T4021	arg2Of	E,(
R3211	T4024	T4022	arg1Of	E,Fig.
R3212	T4024	T4023	arg1Of	E,1
R3213	T4024	T4026	modOf	E,see
R3214	T4026	T4025	arg1Of	see,;
R3215	T4026	T4027	arg1Of	see,also
R3216	T4028	T4029	arg1Of	Fig.,2
R3217	T4030	T4026	arg2Of	A,see
R3218	T4030	T4028	arg1Of	A,Fig.
R3219	T4031	T4021	arg3Of	),(
R322	T393	T396	arg1Of	expression,as
R3220	T4032	T4033	arg1Of	We,evaluated
R3221	T4033	T4037	arg1Of	evaluated,in
R3222	T4036	T4033	arg2Of	function,evaluated
R3223	T4036	T4034	arg1Of	function,antigen-dependent
R3224	T4036	T4035	arg1Of	function,cytolytic
R3225	T4040	T4037	arg2Of	assay,in
R3226	T4040	T4038	arg1Of	assay,a
R3227	T4040	T4039	arg1Of	assay,short-term
R3228	T4040	T4041	arg2Of	assay,in
R3229	T4040	T4042	arg1Of	assay,which
R323	T396	T391	arg2Of	as,regulates
R3230	T4045	T4043	arg1Of	death,target
R3231	T4045	T4044	arg1Of	death,cell
R3232	T4045	T4046	arg1Of	death,was
R3233	T4045	T4047	arg2Of	death,measured
R3234	T4047	T4041	arg1Of	measured,in
R3235	T4047	T4046	arg2Of	measured,was
R3236	T4047	T4048	arg1Of	measured,within
R3237	T4050	T4048	arg2Of	h,within
R3238	T4050	T4049	arg1Of	h,2
R3239	T4050	T4051	arg1Of	h,(
R324	T396	T392	arg1Of	as,Eomes
R3240	T4054	T4051	arg2Of	F,(
R3241	T4054	T4052	arg1Of	F,Fig.
R3242	T4054	T4053	arg1Of	F,1
R3243	T4055	T4051	arg3Of	),(
R3244	T4057	T4056	arg2Of	limiting,By
R3245	T4059	T4057	arg2Of	duration,limiting
R3246	T4059	T4058	arg1Of	duration,the
R3247	T4059	T4060	arg1Of	duration,of
R3248	T4062	T4060	arg2Of	stimulation,of
R3249	T4062	T4061	arg1Of	stimulation,TCR
R325	T396	T394	arg1Of	as,as
R3250	T4065	T4057	arg1Of	strategy,limiting
R3251	T4065	T4064	arg1Of	strategy,this
R3252	T4065	T4066	arg1Of	strategy,minimizes
R3253	T4066	T4056	arg1Of	minimizes,By
R3254	T4066	T4063	arg1Of	minimizes,","
R3255	T4066	T4073	arg1Of	minimizes,during
R3256	T4067	T4066	arg2Of	cytolysis,minimizes
R3257	T4067	T4068	arg1Of	cytolysis,secondary
R3258	T4068	T4069	arg1Of	secondary,to
R3259	T4072	T4069	arg2Of	expression,to
R326	T396	T395	arg1Of	as,well
R3260	T4072	T4070	arg1Of	expression,new
R3261	T4072	T4071	arg1Of	expression,gene
R3262	T4075	T4073	arg2Of	period,during
R3263	T4075	T4074	arg1Of	period,the
R3264	T4075	T4076	arg1Of	period,of
R3265	T4078	T4076	arg2Of	assay,of
R3266	T4078	T4077	arg1Of	assay,the
R3267	T4081	T4079	arg1Of	cells,Naive
R3268	T4081	T4080	arg1Of	cells,T
R3269	T4081	T4082	arg1Of	cells,did
R327	T397	T396	arg2Of	expression,as
R3270	T4081	T4084	arg1Of	cells,display
R3271	T4084	T4082	arg2Of	display,did
R3272	T4084	T4083	arg1Of	display,not
R3273	T4084	T4088	arg1Of	display,in
R3274	T4084	T4096	arg1Of	display,","
R3275	T4084	T4099	arg1Of	display,because
R3276	T4086	T4085	arg1Of	cytolytic,significant
R3277	T4087	T4084	arg2Of	function,display
R3278	T4087	T4086	arg1Of	function,cytolytic
R3279	T4091	T4088	arg2Of	assay,in
R328	T397	T398	arg1Of	expression,of
R3280	T4091	T4089	arg1Of	assay,this
R3281	T4091	T4090	arg1Of	assay,short-term
R3282	T4091	T4092	arg1Of	assay,(
R3283	T4094	T4092	arg2Of	data,(
R3284	T4094	T4093	arg1Of	data,unpublished
R3285	T4095	T4092	arg3Of	),(
R3286	T4098	T4097	arg1Of	likely,most
R3287	T4099	T4098	arg1Of	because,likely
R3288	T4100	T4101	arg1Of	they,express
R3289	T4100	T4110	arg1Of	they,lack
R329	T401	T398	arg2Of	markers,of
R3290	T4101	T4109	arg1Of	express,and
R3291	T4102	T4103	arg1Of	immature,(
R3292	T4104	T4103	arg2Of	unprocessed,(
R3293	T4105	T4103	arg3Of	),(
R3294	T4106	T4101	arg2Of	forms,express
R3295	T4106	T4102	arg1Of	forms,immature
R3296	T4106	T4107	arg1Of	forms,of
R3297	T4108	T4107	arg2Of	perforin,of
R3298	T4109	T4099	arg2Of	and,because
R3299	T4110	T4109	arg2Of	lack,and
R330	T401	T399	arg1Of	markers,three
R3300	T4110	T4115	arg1Of	lack,(
R3301	T4112	T4110	arg2Of	capacity,lack
R3302	T4112	T4111	arg1Of	capacity,the
R3303	T4112	T4113	modOf	capacity,to
R3304	T4114	T4113	arg1Of	degranulate,to
R3305	T4116	T4115	arg2Of	18,(
R3306	T4116	T4117	arg1Of	18,","
R3307	T4118	T4117	arg2Of	19,","
R3308	T4119	T4115	arg3Of	),(
R3309	T4121	T4120	arg1Of	after,Even
R331	T401	T400	arg1Of	markers,cardinal
R3310	T4122	T4121	arg2Of	activation,after
R3311	T4122	T4123	arg1Of	activation,for
R3312	T4124	T4125	arg1Of	2,or
R3313	T4126	T4125	arg2Of	4,or
R3314	T4127	T4123	arg2Of	d,for
R3315	T4127	T4124	arg1Of	d,2
R3316	T4127	T4126	arg1Of	d,4
R3317	T4130	T4129	arg1Of	cells,the
R3318	T4130	T4131	arg1Of	cells,showed
R3319	T4131	T4121	arg1Of	showed,after
R332	T401	T402	arg1Of	markers,of
R3320	T4131	T4128	arg1Of	showed,","
R3321	T4133	T4132	arg1Of	cytolytic,poor
R3322	T4134	T4131	arg2Of	activity,showed
R3323	T4134	T4133	arg1Of	activity,cytolytic
R3324	T4134	T4135	arg1Of	activity,(
R3325	T4134	T4140	arg1Of	activity,","
R3326	T4134	T4141	arg1Of	activity,in
R3327	T4138	T4135	arg2Of	F,(
R3328	T4138	T4136	arg1Of	F,Fig.
R3329	T4138	T4137	arg1Of	F,1
R333	T401	T407	arg1Of	markers,:
R3330	T4139	T4135	arg3Of	),(
R3331	T4143	T4141	arg2Of	contrast,in
R3332	T4143	T4142	arg1Of	contrast,striking
R3333	T4143	T4144	arg1Of	contrast,to
R3334	T4146	T4144	arg2Of	capacity,to
R3335	T4146	T4145	arg1Of	capacity,their
R3336	T4146	T4147	arg1Of	capacity,for
R3337	T4150	T4147	arg2Of	production,for
R3338	T4150	T4148	arg1Of	production,efficient
R3339	T4150	T4149	arg1Of	production,cytokine
R334	T406	T402	arg2Of	program,of
R3340	T4150	T4151	arg1Of	production,(
R3341	T4154	T4151	arg2Of	E,(
R3342	T4154	T4152	arg1Of	E,Fig.
R3343	T4154	T4153	arg1Of	E,1
R3344	T4155	T4151	arg3Of	),(
R3345	T4157	T4156	arg1Of	cells,Only
R3346	T4157	T4158	arg2Of	cells,cultured
R3347	T4157	T4162	arg1Of	cells,displayed
R3348	T4158	T4159	arg1Of	cultured,until
R3349	T4160	T4159	arg2Of	day,until
R335	T406	T403	arg1Of	program,the
R3350	T4160	T4161	arg1Of	day,6
R3351	T4162	T4165	arg1Of	displayed,","
R3352	T4162	T4166	arg1Of	displayed,as
R3353	T4164	T4162	arg2Of	cytotoxicity,displayed
R3354	T4164	T4163	arg1Of	cytotoxicity,robust
R3355	T4167	T4166	arg2Of	judged,as
R3356	T4170	T4167	arg1Of	ability,judged
R3357	T4170	T4168	arg2Of	ability,by
R3358	T4170	T4169	arg1Of	ability,their
R3359	T4170	T4171	modOf	ability,to
R336	T406	T404	arg1Of	program,effector
R3360	T4170	T4181	arg1Of	ability,(
R3361	T4172	T4171	arg1Of	induce,to
R3362	T4172	T4174	arg1Of	induce,in
R3363	T4173	T4172	arg2Of	apoptosis,induce
R3364	T4177	T4174	arg2Of	number,in
R3365	T4177	T4175	arg1Of	number,a
R3366	T4177	T4176	arg1Of	number,large
R3367	T4177	T4178	arg1Of	number,of
R3368	T4180	T4178	arg2Of	cells,of
R3369	T4180	T4179	arg1Of	cells,target
R337	T406	T405	arg1Of	program,CTL
R3370	T4184	T4181	arg2Of	F,(
R3371	T4184	T4182	arg1Of	F,Fig.
R3372	T4184	T4183	arg1Of	F,1
R3373	T4185	T4181	arg3Of	),(
R3374	T4187	T4186	arg1Of	results,These
R3375	T4187	T4188	arg1Of	results,show
R3376	T4194	T4190	arg2Of	stimulus,after
R3377	T4194	T4191	arg1Of	stimulus,a
R3378	T4194	T4192	arg1Of	stimulus,strong
R3379	T4194	T4193	arg1Of	stimulus,priming
R338	T408	T409	arg1Of	IFN-γ,","
R3380	T4194	T4195	arg1Of	stimulus,through
R3381	T4196	T4197	arg1Of	TCRs,and
R3382	T4197	T4195	arg2Of	and,through
R3383	T4197	T4201	arg1Of	and,vitro
R3384	T4199	T4197	arg2Of	receptors,and
R3385	T4199	T4198	arg1Of	receptors,co-stimulatory
R3386	T4201	T4200	arg1Of	vitro,in
R3387	T4205	T4203	arg1Of	expression,granzyme
R3388	T4205	T4204	arg1Of	expression,B
R3389	T4205	T4206	arg1Of	expression,and
R339	T409	T412	arg1Of	",",and
R3390	T4206	T4213	arg1Of	and,are
R3391	T4206	T4214	arg2Of	and,programmed
R3392	T4208	T4206	arg2Of	ability,and
R3393	T4208	T4207	arg1Of	ability,the
R3394	T4208	T4209	modOf	ability,to
R3395	T4210	T4209	arg1Of	produce,to
R3396	T4212	T4210	arg2Of	cytokines,produce
R3397	T4212	T4211	arg1Of	cytokines,effector
R3398	T4214	T4188	arg2Of	programmed,show
R3399	T4214	T4189	arg1Of	programmed,that
R340	T410	T409	arg2Of	perforin,","
R3400	T4214	T4190	arg1Of	programmed,after
R3401	T4214	T4202	arg1Of	programmed,","
R3402	T4214	T4213	arg2Of	programmed,are
R3403	T4214	T4215	arg1Of	programmed,early
R3404	T4214	T4216	arg1Of	programmed,","
R3405	T4214	T4217	arg1Of	programmed,whereas
R3406	T4214	T4226	arg1Of	programmed,","
R3407	T4214	T4227	arg1Of	programmed,during
R3408	T4219	T4218	arg1Of	expression,perforin
R3409	T4219	T4220	arg1Of	expression,and
R341	T412	T407	arg2Of	and,:
R3410	T4220	T4223	arg1Of	and,are
R3411	T4220	T4224	arg2Of	and,induced
R3412	T4222	T4220	arg2Of	function,and
R3413	T4222	T4221	arg1Of	function,cytolytic
R3414	T4224	T4217	arg2Of	induced,whereas
R3415	T4224	T4223	arg2Of	induced,are
R3416	T4224	T4225	arg1Of	induced,later
R3417	T4229	T4227	arg2Of	phase,during
R3418	T4229	T4228	arg1Of	phase,the
R3419	T4229	T4230	arg1Of	phase,of
R342	T412	T411	arg1Of	and,","
R3420	T4232	T4230	arg2Of	expansion,of
R3421	T4232	T4231	arg1Of	expansion,clonal
R3422	T4232	T4233	arg1Of	expansion,in
R3423	T4234	T4233	arg2Of	IL-2,in
R3424	T4241	T4237	arg1Of	functions,the
R3425	T4241	T4238	arg1Of	functions,two
R3426	T4241	T4239	arg1Of	functions,major
R3427	T4241	T4240	arg1Of	functions,effector
R3428	T4241	T4242	arg1Of	functions,of
R3429	T4241	T4251	arg1Of	functions,are
R343	T414	T412	arg2Of	B.,and
R3430	T4241	T4254	arg2Of	functions,coregulated
R3431	T4243	T4244	arg1Of	CTL,","
R3432	T4244	T4242	arg2Of	",",of
R3433	T4246	T4245	arg1Of	production,cytokine
R3434	T4246	T4247	arg1Of	production,and
R3435	T4247	T4244	arg2Of	and,","
R3436	T4249	T4247	arg2Of	activity,and
R3437	T4249	T4248	arg1Of	activity,cytolytic
R3438	T4254	T4235	arg1Of	coregulated,Therefore
R3439	T4254	T4236	arg1Of	coregulated,","
R344	T414	T413	arg1Of	B.,granzyme
R3440	T4254	T4250	arg1Of	coregulated,","
R3441	T4254	T4251	arg2Of	coregulated,are
R3442	T4254	T4252	arg1Of	coregulated,not
R3443	T4254	T4253	arg1Of	coregulated,intrinsically
R345	T416	T415	arg1Of	data,Our
R346	T416	T417	arg1Of	data,point
R347	T417	T391	arg3Of	point,regulates
R348	T417	T418	arg1Of	point,to
R349	T420	T418	arg2Of	existence,to
R350	T420	T419	arg1Of	existence,the
R351	T420	T421	arg1Of	existence,of
R352	T420	T426	arg2Of	existence,in
R353	T420	T427	arg1Of	existence,which
R354	T425	T421	arg2Of	network,of
R355	T425	T422	arg1Of	network,an
R356	T425	T423	arg1Of	network,elaborate
R357	T425	T424	arg1Of	network,transcriptional
R358	T428	T430	arg1Of	Runx3,induces
R359	T428	T433	arg1Of	Runx3,cooperates
R360	T428	T439	arg1Of	Runx3,regulate
R361	T430	T431	arg1Of	induces,and
R362	T431	T426	arg1Of	and,in
R363	T431	T429	arg1Of	and,initially
R364	T433	T431	arg2Of	cooperates,and
R365	T433	T432	arg1Of	cooperates,then
R366	T433	T434	arg1Of	cooperates,with
R367	T433	T438	modOf	cooperates,to
R368	T437	T434	arg2Of	factors,with
R369	T437	T435	arg1Of	factors,T-box
R370	T437	T436	arg1Of	factors,transcription
R371	T439	T438	arg1Of	regulate,to
R372	T441	T439	arg2Of	transcription,regulate
R373	T441	T440	arg1Of	transcription,gene
R374	T441	T442	arg1Of	transcription,in
R375	T444	T442	arg2Of	CTLs,in
R376	T444	T443	arg1Of	CTLs,differentiating
R4074	T5121	T5120	arg1Of	kinetics,Distinct
R4075	T5121	T5122	arg1Of	kinetics,of
R4076	T5121	T5127	arg1Of	kinetics,during
R4077	T5123	T5124	arg1Of	T-bet,and
R4078	T5125	T5124	arg2Of	Eomes,and
R4079	T5126	T5122	arg2Of	expression,of
R4080	T5126	T5123	arg1Of	expression,T-bet
R4081	T5126	T5125	arg1Of	expression,Eomes
R4082	T5129	T5127	arg2Of	differentiation,during
R4083	T5129	T5128	arg1Of	differentiation,CTL
R4084	T5133	T5131	arg1Of	factors,T-box
R4085	T5133	T5132	arg1Of	factors,transcription
R4086	T5134	T5135	arg1Of	T-bet,and
R4087	T5135	T5130	arg1Of	and,The
R4088	T5135	T5133	arg1Of	and,factors
R4089	T5135	T5137	arg1Of	and,have
R4090	T5135	T5138	arg1Of	and,been
R4091	T5135	T5139	arg2Of	and,linked
R4092	T5136	T5135	arg2Of	Eomes,and
R4093	T5139	T5137	arg2Of	linked,have
R4094	T5139	T5138	arg2Of	linked,been
R4095	T5139	T5140	arg1Of	linked,to
R4096	T5142	T5140	arg2Of	regulation,to
R4097	T5142	T5141	arg1Of	regulation,the
R4098	T5142	T5143	arg1Of	regulation,of
R4099	T5144	T5143	arg2Of	genes,of
R4100	T5144	T5145	arg1Of	genes,encoding
R4101	T5147	T5146	arg1Of	cytokines,effector
R4102	T5147	T5148	arg1Of	cytokines,(
R4103	T5147	T5153	arg1Of	cytokines,and
R4104	T5151	T5148	arg2Of	Ifng,(
R4105	T5151	T5149	arg1Of	Ifng,e.g.
R4106	T5151	T5150	arg1Of	Ifng,","
R4107	T5152	T5148	arg3Of	),(
R4108	T5153	T5145	arg2Of	and,encoding
R4109	T5153	T5159	arg1Of	and,(
R4110	T5153	T5166	arg1Of	and,(
R4111	T5154	T5153	arg2Of	genes,and
R4112	T5154	T5155	arg1Of	genes,important
R4113	T5155	T5156	arg1Of	important,for
R4114	T5158	T5156	arg2Of	function,for
R4115	T5158	T5157	arg1Of	function,cytolytic
R4116	T5162	T5163	arg1Of	Prf1,and
R4117	T5163	T5159	arg2Of	and,(
R4118	T5163	T5160	arg1Of	and,e.g.
R4119	T5163	T5161	arg1Of	and,","
R4120	T5164	T5163	arg2Of	GzmB,and
R4121	T5165	T5159	arg3Of	),(
R4122	T5167	T5166	arg2Of	20,(
R4123	T5168	T5166	arg3Of	),(
R4124	T5169	T5170	arg1Of	We,investigated
R4125	T5172	T5170	arg2Of	kinetics,investigated
R4126	T5172	T5171	arg1Of	kinetics,the
R4127	T5172	T5173	arg1Of	kinetics,of
R4128	T5174	T5173	arg2Of	expression,of
R4129	T5174	T5175	arg1Of	expression,of
R4130	T5178	T5175	arg2Of	factors,of
R4131	T5178	T5176	arg1Of	factors,these
R4132	T5178	T5177	arg1Of	factors,transcription
R4133	T5178	T5179	arg1Of	factors,in
R4134	T5182	T5181	arg1Of	vitro,in
R4135	T5183	T5179	arg2Of	cultures,in
R4136	T5183	T5180	arg1Of	cultures,our
R4137	T5183	T5182	arg1Of	cultures,vitro
R4138	T5183	T5184	arg1Of	cultures,(
R4139	T5185	T5184	arg2Of	Fig.,(
R4140	T5185	T5186	arg1Of	Fig.,1
R4141	T5185	T5187	arg1Of	Fig.,","
R4142	T5188	T5187	arg2Of	A–D,","
R4143	T5189	T5184	arg3Of	),(
R4144	T5191	T5190	arg1Of	mRNA,T-bet
R4145	T5191	T5192	arg1Of	mRNA,and
R4146	T5192	T5194	arg1Of	and,were
R4147	T5192	T5196	arg1Of	and,detectable
R4148	T5192	T5204	arg1Of	and,were
R4149	T5192	T5206	arg2Of	and,induced
R4150	T5192	T5215	arg1Of	and,remained
R4151	T5192	T5216	arg2Of	and,expressed
R4152	T5193	T5192	arg2Of	protein,and
R4153	T5194	T5195	arg1Of	were,not
R4154	T5194	T5203	arg1Of	were,but
R4155	T5196	T5194	arg2Of	detectable,were
R4156	T5196	T5197	arg1Of	detectable,in
R4157	T5201	T5197	arg2Of	cells,in
R4158	T5201	T5198	arg1Of	cells,naive
R4159	T5201	T5199	arg1Of	cells,CD8+
R4160	T5201	T5200	arg1Of	cells,T
R4161	T5203	T5202	arg1Of	but,","
R4162	T5203	T5214	arg1Of	but,and
R4163	T5206	T5203	arg2Of	induced,but
R4164	T5206	T5204	arg2Of	induced,were
R4165	T5206	T5205	arg1Of	induced,strongly
R4166	T5206	T5207	arg1Of	induced,upon
R4167	T5209	T5207	arg2Of	priming,upon
R4168	T5209	T5208	arg1Of	priming,TCR
R4169	T5209	T5210	arg1Of	priming,(
R4170	T5211	T5210	arg2Of	day,(
R4171	T5211	T5212	arg1Of	day,2
R4172	T5213	T5210	arg3Of	),(
R4173	T5216	T5214	arg2Of	expressed,and
R4174	T5216	T5215	arg2Of	expressed,remained
R4175	T5216	T5217	arg1Of	expressed,through
R4176	T5218	T5217	arg2Of	day,through
R4177	T5218	T5219	arg1Of	day,6
R4178	T5218	T5220	arg1Of	day,of
R4179	T5221	T5220	arg2Of	differentiation,of
R4180	T5221	T5222	arg1Of	differentiation,(
R4181	T5223	T5222	arg2Of	Fig.,(
R4182	T5223	T5224	arg1Of	Fig.,1
R4183	T5223	T5225	arg1Of	Fig.,","
R4184	T5223	T5234	arg1Of	Fig.,","
R4185	T5226	T5227	arg1Of	A,and
R4186	T5227	T5225	arg2Of	and,","
R4187	T5227	T5230	arg2Of	and,quantified
R4188	T5228	T5227	arg2Of	C,and
R4189	T5230	T5229	arg1Of	quantified,;
R4190	T5230	T5231	arg1Of	quantified,in
R4191	T5232	T5231	arg2Of	Fig.,in
R4192	T5232	T5233	arg1Of	Fig.,1
R4193	T5235	T5236	arg1Of	B,and
R4194	T5236	T5234	arg2Of	and,","
R4195	T5237	T5236	arg2Of	D,and
R4196	T5238	T5222	arg3Of	),(
R4197	T5240	T5239	arg2Of	contrast,In
R4198	T5243	T5242	arg1Of	expression,Eomes
R4199	T5243	T5244	arg1Of	expression,was
R4200	T5243	T5245	arg1Of	expression,low
R4201	T5243	T5247	arg1Of	expression,undetectable
R4202	T5244	T5239	arg1Of	was,In
R4203	T5244	T5241	arg1Of	was,","
R4204	T5244	T5248	arg1Of	was,at
R4205	T5244	T5261	arg1Of	was,and
R4206	T5245	T5246	arg1Of	low,or
R4207	T5246	T5244	arg2Of	or,was
R4208	T5247	T5246	arg2Of	undetectable,or
R4209	T5251	T5252	arg1Of	mRNA,and
R4210	T5253	T5252	arg2Of	protein,and
R4211	T5254	T5248	arg2Of	levels,at
R4212	T5254	T5249	arg1Of	levels,both
R4213	T5254	T5250	arg1Of	levels,the
R4214	T5254	T5251	arg1Of	levels,mRNA
R4215	T5254	T5253	arg1Of	levels,protein
R4216	T5254	T5255	arg1Of	levels,in
R4217	T5259	T5255	arg2Of	cells,in
R4218	T5259	T5256	arg1Of	cells,naive
R4219	T5259	T5257	arg1Of	cells,CD8+
R4220	T5259	T5258	arg1Of	cells,T
R4221	T5261	T5260	arg1Of	and,","
R4222	T5263	T5262	arg1Of	priming,TCR
R4223	T5263	T5264	arg1Of	priming,in
R4224	T5263	T5266	arg1Of	priming,had
R4225	T5265	T5264	arg2Of	culture,in
R4226	T5266	T5261	arg2Of	had,and
R4227	T5266	T5271	arg1Of	had,on
R4228	T5270	T5266	arg2Of	effect,had
R4229	T5270	T5267	arg1Of	effect,only
R4230	T5270	T5268	arg1Of	effect,a
R4231	T5270	T5269	arg1Of	effect,modest
R4232	T5273	T5271	arg2Of	expression,on
R4233	T5273	T5272	arg1Of	expression,its
R4234	T5273	T5274	arg1Of	expression,at
R4235	T5275	T5274	arg2Of	day,at
R4236	T5275	T5276	arg1Of	day,2
R4237	T5275	T5277	arg1Of	day,(
R4238	T5278	T5277	arg2Of	Fig.,(
R4239	T5278	T5279	arg1Of	Fig.,1
R4240	T5278	T5280	arg1Of	Fig.,","
R4241	T5281	T5282	arg1Of	A,and
R4242	T5282	T5280	arg2Of	and,","
R4243	T5283	T5282	arg2Of	C,and
R4244	T5284	T5277	arg3Of	),(
R4245	T5286	T5285	arg1Of	induction,Strong
R4246	T5286	T5287	arg1Of	induction,of
R4247	T5286	T5292	arg1Of	induction,was
R4248	T5286	T5294	arg2Of	induction,observed
R4249	T5289	T5290	arg1Of	mRNA,and
R4250	T5290	T5287	arg2Of	and,of
R4251	T5290	T5288	arg1Of	and,Eomes
R4252	T5291	T5290	arg2Of	protein,and
R4253	T5294	T5292	arg2Of	observed,was
R4254	T5294	T5293	arg1Of	observed,only
R4255	T5294	T5295	arg1Of	observed,at
R4256	T5294	T5300	arg1Of	observed,(
R4257	T5296	T5297	arg1Of	day,4
R4258	T5296	T5298	arg1Of	day,and
R4259	T5299	T5295	arg2Of	later,at
R4260	T5299	T5296	arg1Of	later,day
R4261	T5301	T5300	arg2Of	Fig.,(
R4262	T5301	T5302	arg1Of	Fig.,1
R4263	T5301	T5303	arg1Of	Fig.,","
R4264	T5304	T5305	arg1Of	A,and
R4265	T5305	T5303	arg2Of	and,","
R4266	T5306	T5305	arg2Of	C,and
R4267	T5307	T5300	arg3Of	),(
R4268	T5310	T5308	arg1Of	expression,T-bet
R4269	T5310	T5309	arg1Of	expression,mRNA
R4270	T5310	T5312	arg1Of	expression,preceded
R4271	T5312	T5311	arg1Of	preceded,slightly
R4272	T5312	T5318	arg1Of	preceded,;
R4273	T5314	T5312	arg2Of	expression,preceded
R4274	T5314	T5313	arg1Of	expression,the
R4275	T5314	T5315	arg1Of	expression,of
R4276	T5317	T5315	arg2Of	mRNA,of
R4277	T5317	T5316	arg1Of	mRNA,GzmB
R4278	T5322	T5323	arg1Of	mRNA,and
R4279	T5323	T5321	arg1Of	and,Eomes
R4280	T5323	T5325	arg1Of	and,were
R4281	T5323	T5326	arg2Of	and,expressed
R4282	T5324	T5323	arg2Of	protein,and
R4283	T5326	T5318	arg2Of	expressed,;
R4284	T5326	T5319	arg1Of	expressed,similarly
R4285	T5326	T5320	arg1Of	expressed,","
R4286	T5326	T5325	arg2Of	expressed,were
R4287	T5326	T5327	arg1Of	expressed,∼1
R4288	T5326	T5340	arg1Of	expressed,(
R4289	T5327	T5328	arg1Of	∼1,d
R4290	T5327	T5329	arg1Of	∼1,ahead
R4291	T5327	T5338	arg1Of	∼1,","
R4292	T5327	T5339	arg1Of	∼1,respectively
R4293	T5329	T5330	arg1Of	ahead,of
R4294	T5332	T5330	arg2Of	reexpression,of
R4295	T5332	T5331	arg1Of	reexpression,the
R4296	T5332	T5333	arg1Of	reexpression,of
R4297	T5335	T5334	arg1Of	mRNA,perforin
R4298	T5335	T5336	arg1Of	mRNA,and
R4299	T5336	T5333	arg2Of	and,of
R4300	T5337	T5336	arg2Of	protein,and
R4301	T5341	T5342	arg1Of	Fig.,1
R4302	T5341	T5343	arg1Of	Fig.,","
R4303	T5343	T5345	arg1Of	",",and
R4304	T5344	T5343	arg2Of	B,","
R4305	T5345	T5340	arg2Of	and,(
R4306	T5346	T5345	arg2Of	D,and
R4307	T5347	T5340	arg3Of	),(
R4308	T5351	T5348	arg1Of	analysis,This
R4309	T5351	T5349	arg1Of	analysis,detailed
R4310	T5351	T5350	arg1Of	analysis,kinetic
R4311	T5351	T5352	arg1Of	analysis,suggested
R4312	T5358	T5355	arg2Of	conditions,under
R4313	T5358	T5356	arg1Of	conditions,our
R4314	T5358	T5357	arg1Of	conditions,culture
R4315	T5360	T5361	arg1Of	T-bet,and
R4316	T5361	T5363	arg1Of	and,contribute
R4317	T5362	T5361	arg2Of	Eomes,and
R4318	T5363	T5352	arg2Of	contribute,suggested
R4319	T5363	T5353	arg1Of	contribute,that
R4320	T5363	T5354	arg1Of	contribute,","
R4321	T5363	T5355	arg1Of	contribute,under
R4322	T5363	T5359	arg1Of	contribute,","
R4323	T5363	T5364	arg1Of	contribute,to
R4324	T5363	T5370	arg1Of	contribute,during
R4325	T5366	T5364	arg2Of	aspects,to
R4326	T5366	T5365	arg1Of	aspects,distinct
R4327	T5366	T5367	arg1Of	aspects,of
R4328	T5369	T5367	arg2Of	transcription,of
R4329	T5369	T5368	arg1Of	transcription,gene
R4330	T5372	T5370	arg2Of	differentiation,during
R4331	T5372	T5371	arg1Of	differentiation,CTL
R4332	T5373	T5374	arg1Of	T-bet,is
R4333	T5373	T5375	arg2Of	T-bet,required
R4334	T5375	T5374	arg2Of	required,is
R4335	T5375	T5376	arg1Of	required,early
R4336	T5375	T5377	arg1Of	required,for
R4337	T5375	T5381	arg1Of	required,and
R4338	T5379	T5377	arg2Of	production,for
R4339	T5379	T5378	arg1Of	production,IFN-γ
R4340	T5381	T5380	arg1Of	and,","
R4341	T5383	T5382	arg1Of	data,our
R4342	T5383	T5384	arg1Of	data,suggested
R4343	T5384	T5381	arg2Of	suggested,and
R4344	T5386	T5387	arg1Of	Eomes,might
R4345	T5386	T5389	arg1Of	Eomes,function
R4346	T5386	T5396	arg1Of	Eomes,might
R4347	T5386	T5397	arg1Of	Eomes,contribute
R4348	T5389	T5387	arg2Of	function,might
R4349	T5389	T5388	arg1Of	function,not
R4350	T5389	T5390	arg1Of	function,during
R4351	T5389	T5394	arg1Of	function,but
R4352	T5393	T5390	arg2Of	period,during
R4353	T5393	T5391	arg1Of	period,this
R4354	T5393	T5392	arg1Of	period,early
R4355	T5394	T5384	arg2Of	but,suggested
R4356	T5394	T5385	arg1Of	but,that
R4357	T5397	T5394	arg2Of	contribute,but
R4358	T5397	T5395	arg1Of	contribute,rather
R4359	T5397	T5396	arg2Of	contribute,might
R4360	T5397	T5399	arg1Of	contribute,to
R4361	T5399	T5398	arg1Of	to,later
R4362	T5401	T5399	arg2Of	control,to
R4363	T5401	T5400	arg1Of	control,the
R4364	T5401	T5402	arg1Of	control,of
R4365	T5404	T5402	arg2Of	expression,of
R4366	T5404	T5403	arg1Of	expression,perforin
R4367	T5406	T5405	arg1Of	data,Our
R4368	T5406	T5407	arg1Of	data,seemed
R4369	T5406	T5409	arg1Of	data,consistent
R4370	T5407	T5432	arg1Of	seemed,;
R4371	T5409	T5407	arg2Of	consistent,seemed
R4372	T5409	T5408	arg1Of	consistent,most
R4373	T5409	T5410	arg1Of	consistent,with
R4374	T5412	T5410	arg2Of	model,with
R4375	T5412	T5411	arg1Of	model,a
R4376	T5412	T5413	arg2Of	model,in
R4377	T5412	T5414	arg1Of	model,which
R4378	T5412	T5419	arg1Of	model,","
R4379	T5412	T5420	arg1Of	model,which
R4380	T5412	T5423	arg1Of	model,induces
R4381	T5416	T5415	arg1Of	signals,TCR
R4382	T5416	T5417	arg1Of	signals,induce
R4383	T5417	T5413	arg1Of	induce,in
R4384	T5418	T5417	arg2Of	T-bet,induce
R4385	T5422	T5421	arg2Of	turn,in
R4386	T5423	T5421	arg1Of	induces,in
R4387	T5424	T5425	arg1Of	IFN-γ,(
R4388	T5424	T5428	arg1Of	IFN-γ,and
R4389	T5426	T5425	arg2Of	17,(
R4390	T5427	T5425	arg3Of	),(
R4391	T5428	T5423	arg2Of	and,induces
R4392	T5431	T5428	arg2Of	B,and
R4393	T5431	T5429	arg1Of	B,possibly
R4394	T5431	T5430	arg1Of	B,granzyme
R4395	T5435	T5436	arg1Of	Eomes,is
R4396	T5435	T5437	arg2Of	Eomes,induced
R4397	T5435	T5447	arg1Of	Eomes,activates
R4398	T5437	T5436	arg2Of	induced,is
R4399	T5437	T5438	arg1Of	induced,during
R4400	T5437	T5446	arg1Of	induced,and
R4401	T5440	T5438	arg2Of	period,during
R4402	T5440	T5439	arg1Of	period,the
R4403	T5440	T5441	arg1Of	period,of
R4404	T5443	T5441	arg2Of	expansion,of
R4405	T5443	T5442	arg1Of	expansion,clonal
R4406	T5443	T5444	arg1Of	expansion,in
R4407	T5445	T5444	arg2Of	IL-2,in
R4408	T5446	T5432	arg2Of	and,;
R4409	T5446	T5433	arg1Of	and,subsequently
R4410	T5446	T5434	arg1Of	and,","
R4411	T5447	T5446	arg2Of	activates,and
R4412	T5449	T5447	arg2Of	expression,activates
R4413	T5449	T5448	arg1Of	expression,perforin
R5177	T6493	T6494	arg1Of	Perforin,and
R5178	T6494	T6498	arg1Of	and,are
R5179	T6494	T6501	arg2Of	and,regulated
R5180	T6497	T6494	arg2Of	expression,and
R5181	T6497	T6495	arg1Of	expression,granzyme
R5182	T6497	T6496	arg1Of	expression,B
R5183	T6501	T6498	arg2Of	regulated,are
R5184	T6501	T6499	arg1Of	regulated,not
R5185	T6501	T6500	arg1Of	regulated,appreciably
R5186	T6503	T6501	arg1Of	T-bet,regulated
R5187	T6503	T6502	arg2Of	T-bet,by
R5188	T6505	T6504	arg1Of	test,To
R5189	T6507	T6505	arg2Of	model,test
R5190	T6507	T6506	arg1Of	model,the
R5191	T6507	T6508	arg2Of	model,outlined
R5192	T6508	T6509	arg1Of	outlined,in
R5193	T6508	T6513	arg1Of	outlined,directly
R5194	T6512	T6509	arg2Of	paragraph,in
R5195	T6512	T6510	arg1Of	paragraph,the
R5196	T6512	T6511	arg1Of	paragraph,previous
R5197	T6515	T6505	arg1Of	we,test
R5198	T6515	T6516	arg1Of	we,compared
R5199	T6516	T6504	modOf	compared,To
R5200	T6516	T6514	arg1Of	compared,","
R5201	T6518	T6516	arg2Of	expression,compared
R5202	T6518	T6517	arg1Of	expression,the
R5203	T6518	T6519	arg1Of	expression,of
R5204	T6518	T6527	arg1Of	expression,in
R5205	T6520	T6521	arg1Of	IFN-γ,","
R5206	T6521	T6524	arg1Of	",",and
R5207	T6522	T6521	arg2Of	perforin,","
R5208	T6524	T6519	arg2Of	and,of
R5209	T6524	T6523	arg1Of	and,","
R5210	T6526	T6524	arg2Of	B,and
R5211	T6526	T6525	arg1Of	B,granzyme
R5212	T6530	T6527	arg2Of	cells,in
R5213	T6530	T6528	arg1Of	cells,CD8+
R5214	T6530	T6529	arg1Of	cells,T
R5215	T6530	T6531	arg1Of	cells,from
R5216	T6532	T6533	arg1Of	WT,and
R5217	T6534	T6533	arg2Of	Tbx21,and
R5218	T6536	T6535	arg2Of	T-bet,(
R5219	T6537	T6535	arg3Of	),(
R5220	T6539	T6531	arg2Of	mice,from
R5221	T6539	T6532	arg1Of	mice,WT
R5222	T6539	T6534	arg1Of	mice,Tbx21
R5223	T6539	T6535	arg1Of	mice,(
R5224	T6539	T6538	arg1Of	mice,-deficient
R5225	T6541	T6540	arg2Of	expected,As
R5226	T6541	T6542	arg1Of	expected,(
R5227	T6543	T6544	arg1Of	17,","
R5228	T6544	T6542	arg2Of	",",(
R5229	T6545	T6544	arg2Of	21,","
R5230	T6546	T6542	arg3Of	),(
R5231	T6552	T6548	arg1Of	cells,naive
R5232	T6552	T6549	arg1Of	cells,Tbx21−/−
R5233	T6552	T6550	arg1Of	cells,CD8+
R5234	T6552	T6551	arg1Of	cells,T
R5235	T6552	T6553	arg1Of	cells,produced
R5236	T6553	T6540	arg1Of	produced,As
R5237	T6553	T6547	arg1Of	produced,","
R5238	T6553	T6555	arg1Of	produced,poorly
R5239	T6553	T6556	arg1Of	produced,upon
R5240	T6554	T6553	arg2Of	IFN-γ,produced
R5241	T6557	T6556	arg2Of	activation,upon
R5242	T6557	T6558	arg1Of	activation,(
R5243	T6561	T6558	arg2Of	A,(
R5244	T6561	T6559	arg1Of	A,Fig.
R5245	T6561	T6560	arg1Of	A,2
R5246	T6562	T6558	arg3Of	),(
R5247	T6567	T6565	arg1Of	effect,this
R5248	T6567	T6566	arg1Of	effect,deleterious
R5249	T6567	T6568	arg1Of	effect,of
R5250	T6567	T6571	arg1Of	effect,was
R5251	T6567	T6573	arg2Of	effect,observed
R5252	T6567	T6585	arg1Of	effect,was
R5253	T6567	T6588	arg2Of	effect,mitigated
R5254	T6570	T6568	arg2Of	deficiency,of
R5255	T6570	T6569	arg1Of	deficiency,T-bet
R5256	T6573	T6571	arg2Of	observed,was
R5257	T6573	T6572	arg1Of	observed,only
R5258	T6573	T6574	arg1Of	observed,in
R5259	T6573	T6579	arg1Of	observed,until
R5260	T6573	T6584	arg1Of	observed,but
R5261	T6578	T6574	arg2Of	cells,in
R5262	T6578	T6575	arg1Of	cells,differentiating
R5263	T6578	T6576	arg1Of	cells,CD8+
R5264	T6578	T6577	arg1Of	cells,T
R5265	T6580	T6579	arg2Of	day,until
R5266	T6580	T6581	arg1Of	day,4
R5267	T6580	T6582	arg1Of	day,of
R5268	T6583	T6582	arg2Of	culture,of
R5269	T6584	T6563	arg1Of	but,Notably
R5270	T6584	T6564	arg1Of	but,","
R5271	T6587	T6586	arg1Of	completely,almost
R5272	T6588	T6584	arg2Of	mitigated,but
R5273	T6588	T6585	arg2Of	mitigated,was
R5274	T6588	T6587	arg1Of	mitigated,completely
R5275	T6588	T6589	arg1Of	mitigated,by
R5276	T6590	T6589	arg2Of	day,by
R5277	T6590	T6591	arg1Of	day,6
R5278	T6590	T6592	arg1Of	day,(
R5279	T6595	T6592	arg2Of	A,(
R5280	T6595	T6593	arg1Of	A,Fig.
R5281	T6595	T6594	arg1Of	A,2
R5282	T6596	T6592	arg3Of	),(
R5283	T6599	T6598	arg1Of	likely,most
R5284	T6601	T6597	arg1Of	compensation,This
R5285	T6601	T6599	arg1Of	compensation,likely
R5286	T6601	T6600	arg2Of	compensation,reflected
R5287	T6601	T6602	arg1Of	compensation,by
R5288	T6601	T6604	arg1Of	compensation,","
R5289	T6601	T6605	arg1Of	compensation,which
R5290	T6601	T6606	arg1Of	compensation,was
R5291	T6601	T6608	arg2Of	compensation,induced
R5292	T6603	T6602	arg2Of	Eomes,by
R5293	T6608	T6606	arg2Of	induced,was
R5294	T6608	T6607	arg1Of	induced,strongly
R5295	T6608	T6609	arg1Of	induced,between
R5296	T6611	T6612	arg1Of	4,and
R5297	T6612	T6609	arg2Of	and,between
R5298	T6612	T6610	arg1Of	and,days
R5299	T6612	T6614	arg1Of	and,(
R5300	T6613	T6612	arg2Of	6,and
R5301	T6615	T6614	arg2Of	Fig.,(
R5302	T6615	T6616	arg1Of	Fig.,1
R5303	T6617	T6614	arg3Of	),(
R5304	T6619	T6618	arg2Of	contrast,In
R5305	T6623	T6621	arg1Of	cells,T-bet–deficient
R5306	T6623	T6622	arg1Of	cells,T
R5307	T6623	T6624	arg2Of	cells,cultured
R5308	T6623	T6628	arg1Of	cells,showed
R5309	T6624	T6625	arg1Of	cultured,for
R5310	T6627	T6625	arg2Of	d,for
R5311	T6627	T6626	arg1Of	d,6
R5312	T6628	T6618	arg1Of	showed,In
R5313	T6628	T6620	arg1Of	showed,","
R5314	T6628	T6631	arg1Of	showed,in
R5315	T6630	T6628	arg2Of	defect,showed
R5316	T6630	T6629	arg1Of	defect,no
R5317	T6634	T6631	arg2Of	expression,in
R5318	T6634	T6632	arg1Of	expression,perforin
R5319	T6634	T6633	arg1Of	expression,mRNA
R5320	T6634	T6635	arg1Of	expression,(
R5321	T6638	T6636	arg1Of	B,Fig.
R5322	T6638	T6637	arg1Of	B,2
R5323	T6638	T6640	arg1Of	B,compare
R5324	T6640	T6635	arg2Of	compare,(
R5325	T6640	T6639	arg1Of	compare,","
R5326	T6642	T6643	arg1Of	1,and
R5327	T6643	T6640	arg2Of	and,compare
R5328	T6643	T6641	arg1Of	and,lanes
R5329	T6644	T6643	arg2Of	4,and
R5330	T6645	T6635	arg3Of	),(
R5331	T6646	T6648	arg1Of	We,observed
R5332	T6648	T6647	arg1Of	observed,consistently
R5333	T6648	T6659	arg1Of	observed,(
R5334	T6648	T6670	arg1Of	observed,","
R5335	T6648	T6671	arg1Of	observed,which
R5336	T6648	T6672	arg1Of	observed,did
R5337	T6648	T6674	arg1Of	observed,translate
R5338	T6651	T6648	arg2Of	reduction,observed
R5339	T6651	T6649	arg1Of	reduction,a
R5340	T6651	T6650	arg1Of	reduction,modest
R5341	T6651	T6652	arg1Of	reduction,in
R5342	T6651	T6655	arg1Of	reduction,in
R5343	T6654	T6652	arg2Of	mRNA,in
R5344	T6654	T6653	arg1Of	mRNA,GzmB
R5345	T6658	T6655	arg2Of	cells,in
R5346	T6658	T6656	arg1Of	cells,T-bet–deficient
R5347	T6658	T6657	arg1Of	cells,T
R5348	T6662	T6660	arg1Of	B,Fig.
R5349	T6662	T6661	arg1Of	B,2
R5350	T6662	T6664	arg1Of	B,compare
R5351	T6664	T6659	arg2Of	compare,(
R5352	T6664	T6663	arg1Of	compare,","
R5353	T6666	T6667	arg1Of	1,and
R5354	T6667	T6664	arg2Of	and,compare
R5355	T6667	T6665	arg1Of	and,lanes
R5356	T6668	T6667	arg2Of	4,and
R5357	T6669	T6659	arg3Of	),(
R5358	T6674	T6672	arg2Of	translate,did
R5359	T6674	T6673	arg1Of	translate,not
R5360	T6674	T6675	arg1Of	translate,into
R5361	T6677	T6675	arg2Of	decrease,into
R5362	T6677	T6676	arg1Of	decrease,a
R5363	T6677	T6678	arg1Of	decrease,in
R5364	T6679	T6678	arg2Of	expression,in
R5365	T6679	T6680	arg1Of	expression,of
R5366	T6683	T6680	arg2Of	protein,of
R5367	T6683	T6681	arg1Of	protein,granzyme
R5368	T6683	T6682	arg1Of	protein,B
R5369	T6683	T6684	arg1Of	protein,(
R5370	T6687	T6684	arg2Of	C,(
R5371	T6687	T6685	arg1Of	C,Fig.
R5372	T6687	T6686	arg1Of	C,2
R5373	T6688	T6684	arg3Of	),(
R5374	T6690	T6689	arg1Of	examine,To
R5375	T6692	T6690	arg2Of	role,examine
R5376	T6692	T6691	arg1Of	role,the
R5377	T6692	T6693	arg1Of	role,of
R5378	T6694	T6693	arg2Of	Eomes,of
R5379	T6696	T6697	arg1Of	we,transduced
R5380	T6697	T6689	modOf	transduced,To
R5381	T6697	T6695	arg1Of	transduced,","
R5382	T6701	T6697	arg2Of	cells,transduced
R5383	T6701	T6698	arg1Of	cells,naive
R5384	T6701	T6699	arg1Of	cells,CD8+
R5385	T6701	T6700	arg1Of	cells,T
R5386	T6701	T6702	arg1Of	cells,from
R5387	T6701	T6707	arg1Of	cells,with
R5388	T6703	T6704	arg1Of	WT,and
R5389	T6705	T6704	arg2Of	Tbx21−/−,and
R5390	T6706	T6702	arg2Of	mice,from
R5391	T6706	T6703	arg1Of	mice,WT
R5392	T6706	T6705	arg1Of	mice,Tbx21−/−
R5393	T6708	T6707	arg2Of	retroviruses,with
R5394	T6708	T6709	arg1Of	retroviruses,containing
R5395	T6713	T6710	arg1Of	site,internal
R5396	T6713	T6711	arg1Of	site,ribosome
R5397	T6713	T6712	arg1Of	site,entry
R5398	T6713	T6714	arg1Of	site,(
R5399	T6715	T6714	arg2Of	IRES,(
R5400	T6716	T6714	arg3Of	),(
R5401	T6717	T6709	arg2Of	–GFP,containing
R5402	T6717	T6713	arg1Of	–GFP,site
R5403	T6717	T6718	arg1Of	–GFP,that
R5404	T6717	T6719	arg1Of	–GFP,were
R5405	T6717	T6721	arg1Of	–GFP,empty
R5406	T6717	T6723	arg1Of	–GFP,encoded
R5407	T6717	T6738	arg1Of	–GFP,expanded
R5408	T6719	T6722	arg1Of	were,or
R5409	T6721	T6719	arg2Of	empty,were
R5410	T6721	T6720	arg1Of	empty,either
R5411	T6722	T6737	arg1Of	or,and
R5412	T6723	T6722	arg2Of	encoded,or
R5413	T6726	T6725	arg1Of	transactivating,strongly
R5414	T6727	T6723	arg2Of	version,encoded
R5415	T6727	T6724	arg1Of	version,a
R5416	T6727	T6726	arg1Of	version,transactivating
R5417	T6727	T6728	arg1Of	version,of
R5418	T6729	T6728	arg2Of	Eomes,of
R5419	T6729	T6730	arg1Of	Eomes,(
R5420	T6729	T6733	arg1Of	Eomes,(
R5421	T6731	T6730	arg2Of	Eo-VP16,(
R5422	T6732	T6730	arg3Of	),(
R5423	T6734	T6733	arg2Of	8,(
R5424	T6735	T6733	arg3Of	),(
R5425	T6737	T6736	arg1Of	and,","
R5426	T6738	T6737	arg2Of	expanded,and
R5427	T6738	T6740	arg1Of	expanded,for
R5428	T6738	T6743	arg1Of	expanded,under
R5429	T6739	T6738	arg2Of	them,expanded
R5430	T6742	T6740	arg2Of	d,for
R5431	T6742	T6741	arg1Of	d,6
R5432	T6746	T6743	arg2Of	conditions,under
R5433	T6746	T6744	arg1Of	conditions,our
R5434	T6746	T6745	arg1Of	conditions,culture
R5435	T6747	T6749	arg1Of	Eo-VP16,but
R5436	T6749	T6748	arg1Of	but,","
R5437	T6749	T6750	arg1Of	but,not
R5438	T6749	T6756	arg1Of	but,increased
R5439	T6754	T6749	arg2Of	retrovirus,but
R5440	T6754	T6751	arg1Of	retrovirus,the
R5441	T6754	T6752	arg1Of	retrovirus,empty
R5442	T6754	T6753	arg1Of	retrovirus,GFP
R5443	T6756	T6755	arg1Of	increased,","
R5444	T6758	T6756	arg2Of	expression,increased
R5445	T6758	T6757	arg1Of	expression,perforin
R5446	T6758	T6759	arg1Of	expression,in
R5447	T6766	T6759	arg2Of	cells,in
R5448	T6766	T6760	arg1Of	cells,both
R5449	T6766	T6761	arg1Of	cells,WT
R5450	T6766	T6762	arg1Of	cells,and
R5451	T6766	T6763	arg1Of	cells,T-bet–deficient
R5452	T6766	T6764	arg1Of	cells,CD8+
R5453	T6766	T6765	arg1Of	cells,T
R5454	T6766	T6767	arg1Of	cells,(
R5455	T6770	T6767	arg2Of	B,(
R5456	T6770	T6768	arg1Of	B,Fig.
R5457	T6770	T6769	arg1Of	B,2
R5458	T6770	T6771	arg1Of	B,","
R5459	T6773	T6774	arg1Of	2,","
R5460	T6774	T6776	arg1Of	",",","
R5461	T6775	T6774	arg2Of	3,","
R5462	T6776	T6772	arg1Of	",",lanes
R5463	T6776	T6779	arg1Of	",",and
R5464	T6777	T6776	arg2Of	5,","
R5465	T6779	T6771	arg2Of	and,","
R5466	T6779	T6778	arg1Of	and,","
R5467	T6780	T6779	arg2Of	6,and
R5468	T6781	T6767	arg3Of	),(
R5469	T6783	T6782	arg2Of	expected,As
R5470	T6785	T6787	arg1Of	Eo-VP16,rescued
R5471	T6787	T6782	arg1Of	rescued,As
R5472	T6787	T6784	arg1Of	rescued,","
R5473	T6787	T6786	arg1Of	rescued,also
R5474	T6790	T6787	arg2Of	defect,rescued
R5475	T6790	T6788	arg1Of	defect,the
R5476	T6790	T6789	arg1Of	defect,early
R5477	T6790	T6791	arg1Of	defect,in
R5478	T6793	T6791	arg2Of	production,in
R5479	T6793	T6792	arg1Of	production,IFN-γ
R5480	T6793	T6794	arg2Of	production,observed
R5481	T6794	T6795	arg1Of	observed,in
R5482	T6799	T6795	arg2Of	cells,in
R5483	T6799	T6796	arg1Of	cells,T-bet–deficient
R5484	T6799	T6797	arg1Of	cells,CD8+
R5485	T6799	T6798	arg1Of	cells,T
R5486	T6799	T6800	arg1Of	cells,(
R5487	T6803	T6800	arg2Of	D,(
R5488	T6803	T6801	arg1Of	D,Fig.
R5489	T6803	T6802	arg1Of	D,2
R5490	T6804	T6800	arg3Of	),(
R5491	T6807	T6808	arg1Of	Eo-VP16,did
R5492	T6807	T6810	arg1Of	Eo-VP16,induce
R5493	T6810	T6805	arg1Of	induce,However
R5494	T6810	T6806	arg1Of	induce,","
R5495	T6810	T6808	arg2Of	induce,did
R5496	T6810	T6809	arg1Of	induce,not
R5497	T6810	T6820	arg1Of	induce,;
R5498	T6813	T6810	arg2Of	expression,induce
R5499	T6813	T6811	arg1Of	expression,GzmB
R5500	T6813	T6812	arg1Of	expression,mRNA
R5501	T6813	T6814	arg1Of	expression,in
R5502	T6816	T6817	arg1Of	WT,or
R5503	T6817	T6814	arg2Of	or,in
R5504	T6817	T6815	arg1Of	or,either
R5505	T6819	T6817	arg2Of	cells,or
R5506	T6819	T6818	arg1Of	cells,T-bet–deficient
R5507	T6826	T6823	arg1Of	dependence,the
R5508	T6826	T6824	arg1Of	dependence,partial
R5509	T6826	T6825	arg1Of	dependence,T-bet
R5510	T6826	T6827	arg1Of	dependence,of
R5511	T6826	T6831	arg1Of	dependence,can
R5512	T6826	T6833	arg1Of	dependence,be
R5513	T6826	T6834	arg2Of	dependence,compensated
R5514	T6830	T6827	arg2Of	expression,of
R5515	T6830	T6828	arg1Of	expression,GzmB
R5516	T6830	T6829	arg1Of	expression,mRNA
R5517	T6834	T6820	arg2Of	compensated,;
R5518	T6834	T6821	arg1Of	compensated,thus
R5519	T6834	T6822	arg1Of	compensated,","
R5520	T6834	T6831	arg2Of	compensated,can
R5521	T6834	T6832	arg1Of	compensated,not
R5522	T6834	T6833	arg2Of	compensated,be
R5523	T6834	T6835	arg1Of	compensated,for
R5524	T6837	T6834	arg1Of	Eo-VP16,compensated
R5525	T6837	T6836	arg2Of	Eo-VP16,by
R7154	T8977	T8978	arg1Of	Runx3,controls
R7155	T8980	T8978	arg2Of	aspects,controls
R7156	T8980	T8979	arg1Of	aspects,multiple
R7157	T8980	T8981	arg1Of	aspects,of
R7158	T8980	T8986	arg1Of	aspects,","
R7159	T8980	T8989	arg1Of	aspects,through
R7160	T8985	T8981	arg2Of	program,of
R7161	T8985	T8982	arg1Of	program,the
R7162	T8985	T8983	arg1Of	program,CTL
R7163	T8985	T8984	arg1Of	program,differentiation
R7164	T8988	T8987	arg2Of	part,in
R7165	T8989	T8987	arg1Of	through,in
R7166	T8990	T8989	arg2Of	induction,through
R7167	T8990	T8991	arg1Of	induction,of
R7168	T8992	T8991	arg2Of	Eomes,of
R7169	T8994	T8995	arg1Of	Runx3,is
R7170	T8994	T8997	arg2Of	Runx3,expressed
R7171	T8997	T8993	arg2Of	expressed,Because
R7172	T8997	T8995	arg2Of	expressed,is
R7173	T8997	T8996	arg1Of	expressed,highly
R7174	T8997	T8998	arg1Of	expressed,in
R7175	T8997	T9005	arg1Of	expressed,because
R7176	T8998	T9004	arg1Of	in,and
R7177	T9002	T8998	arg2Of	cells,in
R7178	T9002	T8999	arg1Of	cells,peripheral
R7179	T9002	T9000	arg1Of	cells,CD8+
R7180	T9002	T9001	arg1Of	cells,T
R7181	T9004	T9003	arg1Of	and,","
R7182	T9005	T9004	arg2Of	because,and
R7183	T9005	T9006	arg1Of	because,of
R7184	T9009	T9005	arg2Of	cooperation,because
R7185	T9009	T9007	arg1Of	cooperation,the
R7186	T9009	T9008	arg1Of	cooperation,T-bet-Runx3
R7187	T9009	T9011	arg2Of	cooperation,observed
R7188	T9010	T9011	arg1Of	we,observed
R7189	T9011	T9012	arg1Of	observed,earlier
R7190	T9012	T9013	arg1Of	earlier,in
R7191	T9016	T9013	arg2Of	cells,in
R7192	T9016	T9014	arg1Of	cells,CD4+
R7193	T9016	T9015	arg1Of	cells,T
R7194	T9016	T9017	arg1Of	cells,(
R7195	T9018	T9017	arg2Of	15,(
R7196	T9019	T9017	arg3Of	),(
R7197	T9021	T9022	arg1Of	we,examined
R7198	T9022	T8993	arg1Of	examined,Because
R7199	T9022	T9020	arg1Of	examined,","
R7200	T9024	T9022	arg2Of	role,examined
R7201	T9024	T9023	arg1Of	role,the
R7202	T9024	T9025	arg1Of	role,of
R7203	T9024	T9027	arg1Of	role,in
R7204	T9026	T9025	arg2Of	Runx3,of
R7205	T9030	T9027	arg2Of	differentiation,in
R7206	T9030	T9028	arg1Of	differentiation,effector
R7207	T9030	T9029	arg1Of	differentiation,CTL
R7208	T9031	T9032	arg1Of	We,isolated
R7209	T9032	T9052	arg1Of	isolated,by
R7210	T9035	T9033	arg1Of	cells,CD8+
R7211	T9035	T9034	arg1Of	cells,T
R7212	T9035	T9036	arg1Of	cells,from
R7213	T9035	T9047	arg1Of	cells,and
R7214	T9039	T9038	arg2Of	KO,(
R7215	T9040	T9038	arg3Of	),(
R7216	T9041	T9036	arg2Of	mice,from
R7217	T9041	T9037	arg1Of	mice,Runx3−/−
R7218	T9041	T9038	arg1Of	mice,(
R7219	T9041	T9042	arg1Of	mice,of
R7220	T9046	T9042	arg2Of	background,of
R7221	T9046	T9043	arg1Of	background,the
R7222	T9046	T9044	arg1Of	background,outbred
R7223	T9046	T9045	arg1Of	background,ICR
R7224	T9047	T9032	arg2Of	and,isolated
R7225	T9051	T9047	arg2Of	littermates,and
R7226	T9051	T9048	arg1Of	littermates,their
R7227	T9051	T9049	arg1Of	littermates,WT
R7228	T9051	T9050	arg1Of	littermates,Runx3+/+
R7229	T9054	T9052	arg2Of	selection,by
R7230	T9054	T9053	arg1Of	selection,positive
R7231	T9054	T9055	arg1Of	selection,with
R7232	T9058	T9055	arg2Of	beads,with
R7233	T9058	T9056	arg1Of	beads,anti-CD8
R7234	T9058	T9057	arg1Of	beads,magnetic
R7235	T9058	T9059	arg1Of	beads,(
R7236	T9061	T9062	arg1Of	S1,and
R7237	T9062	T9059	arg2Of	and,(
R7238	T9062	T9060	arg1Of	and,Figs.
R7239	T9062	T9064	arg1Of	and,","
R7240	T9062	T9065	arg1Of	and,available
R7241	T9062	T9068	arg1Of	and,:
R7242	T9063	T9062	arg2Of	S2,and
R7243	T9065	T9066	arg1Of	available,at
R7244	T9067	T9066	arg2Of	http,at
R7245	T9069	T9068	arg2Of	//www.jem.org/cgi/content/full/jem.20081242/DC1,:
R7246	T9070	T9059	arg3Of	),(
R7247	T9076	T9073	arg1Of	cells,Runx3−/−
R7248	T9076	T9074	arg1Of	cells,CD8+
R7249	T9076	T9075	arg1Of	cells,T
R7250	T9076	T9077	arg1Of	cells,were
R7251	T9076	T9079	arg1Of	cells,impaired
R7252	T9077	T9071	arg1Of	were,Strikingly
R7253	T9077	T9072	arg1Of	were,","
R7254	T9077	T9088	arg1Of	were,","
R7255	T9079	T9077	arg2Of	impaired,were
R7256	T9079	T9078	arg1Of	impaired,strongly
R7257	T9079	T9080	arg1Of	impaired,in
R7258	T9082	T9080	arg2Of	ability,in
R7259	T9082	T9081	arg1Of	ability,their
R7260	T9082	T9083	modOf	ability,to
R7261	T9084	T9083	arg1Of	differentiate,to
R7262	T9084	T9085	arg1Of	differentiate,into
R7263	T9087	T9085	arg2Of	CTLs,into
R7264	T9087	T9086	arg1Of	CTLs,effector
R7265	T9089	T9077	arg3Of	as,were
R7266	T9090	T9089	arg2Of	judged,as
R7267	T9092	T9090	arg1Of	expression,judged
R7268	T9092	T9091	arg2Of	expression,by
R7269	T9092	T9093	arg1Of	expression,of
R7270	T9094	T9095	arg1Of	perforin,","
R7271	T9095	T9099	arg1Of	",",and
R7272	T9097	T9095	arg2Of	B,","
R7273	T9097	T9096	arg1Of	B,granzyme
R7274	T9099	T9093	arg2Of	and,of
R7275	T9099	T9098	arg1Of	and,","
R7276	T9100	T9099	arg2Of	IFN-γ,and
R7277	T9100	T9101	arg1Of	IFN-γ,(
R7278	T9102	T9101	arg2Of	Fig.,(
R7279	T9102	T9103	arg1Of	Fig.,3
R7280	T9104	T9101	arg3Of	),(
R7281	T9106	T9105	arg2Of	with,Compared
R7282	T9109	T9106	arg2Of	cells,with
R7283	T9135	T9128	arg3Of	),(
R7284	T9109	T9107	arg1Of	cells,WT
R7285	T9109	T9108	arg1Of	cells,T
R7286	T9138	T9136	arg1Of	cells,Runx3−/−
R7287	T9138	T9137	arg1Of	cells,T
R7288	T9138	T9140	arg1Of	cells,had
R7289	T9112	T9113	arg1Of	mRNA,and
R7290	T9140	T9139	arg1Of	had,also
R7291	T9140	T9145	arg1Of	had,;
R7292	T9143	T9141	arg1Of	Eomes,no
R7293	T9143	T9142	arg1Of	Eomes,detectable
R7294	T9144	T9140	arg2Of	expression,had
R7295	T9144	T9143	arg1Of	expression,Eomes
R7296	T9114	T9113	arg2Of	protein,and
R7297	T9147	T9146	arg2Of	contrast,in
R7298	T9115	T9111	arg1Of	expression,perforin
R7299	T9150	T9149	arg1Of	expression,T-bet
R7300	T9150	T9151	arg1Of	expression,was
R7301	T9150	T9152	arg1Of	expression,unimpaired
R7302	T9151	T9145	arg2Of	was,;
R7303	T9151	T9146	arg1Of	was,in
R7304	T9151	T9148	arg1Of	was,","
R7305	T9152	T9151	arg2Of	unimpaired,was
R7306	T9152	T9153	arg1Of	unimpaired,(
R7307	T9115	T9112	arg1Of	expression,mRNA
R7308	T9115	T9114	arg1Of	expression,protein
R7309	T9115	T9116	arg1Of	expression,were
R7310	T9115	T9118	arg1Of	expression,undetectable
R7311	T9156	T9153	arg2Of	A,(
R7312	T9156	T9154	arg1Of	A,Fig.
R7313	T9156	T9155	arg1Of	A,3
R7314	T9157	T9153	arg3Of	),(
R7315	T9116	T9105	arg1Of	were,Compared
R7316	T9160	T9161	arg1Of	Runx3,was
R7317	T9160	T9162	arg2Of	Runx3,required
R7318	T9162	T9158	arg1Of	required,Furthermore
R7319	T9162	T9159	arg1Of	required,","
R7320	T9162	T9161	arg2Of	required,was
R7321	T9162	T9163	arg1Of	required,for
R7322	T9162	T9174	arg1Of	required,","
R7323	T9162	T9175	arg1Of	required,by
R7324	T9116	T9110	arg1Of	were,","
R7325	T9165	T9163	arg2Of	production,for
R7326	T9165	T9164	arg1Of	production,maximal
R7327	T9165	T9166	arg1Of	production,of
R7328	T9118	T9116	arg2Of	undetectable,were
R7329	T9167	T9169	arg1Of	IFN-γ,but
R7330	T9169	T9166	arg2Of	but,of
R7331	T9169	T9168	arg1Of	but,","
R7332	T9169	T9170	arg1Of	but,not
R7333	T9171	T9172	arg1Of	TNF,or
R7334	T9118	T9117	arg1Of	undetectable,essentially
R7335	T9172	T9169	arg2Of	or,but
R7336	T9173	T9172	arg2Of	IL-2,or
R7337	T9118	T9119	arg1Of	undetectable,in
R7338	T9122	T9119	arg2Of	cells,in
R7339	T9178	T9175	arg2Of	cells,by
R7340	T9178	T9176	arg1Of	cells,CD8+
R7341	T9178	T9177	arg1Of	cells,T
R7342	T9178	T9179	arg2Of	cells,restimulated
R7343	T9179	T9180	arg1Of	restimulated,at
R7344	T9122	T9120	arg1Of	cells,Runx3−/−
R7345	T9181	T9180	arg2Of	day,at
R7346	T9181	T9182	arg1Of	day,6
R7347	T9181	T9183	arg1Of	day,(
R7348	T9122	T9121	arg1Of	cells,T
R7349	T9122	T9123	arg1Of	cells,at
R7350	T9124	T9123	arg2Of	day,at
R7351	T9186	T9183	arg2Of	C,(
R7352	T9186	T9184	arg1Of	C,Fig.
R7353	T9186	T9185	arg1Of	C,3
R7354	T9187	T9183	arg3Of	),(
R7355	T9188	T9190	arg1Of	We,reported
R7356	T9124	T9125	arg1Of	day,6
R7357	T9124	T9126	arg1Of	day,of
R7358	T9127	T9126	arg2Of	culture,of
R7359	T9188	T9208	arg1Of	We,induces
R7360	T9188	T9225	arg1Of	We,promoting
R7361	T9190	T9207	arg1Of	reported,and
R7362	T9127	T9128	arg1Of	culture,(
R7363	T9129	T9128	arg2Of	Fig.,(
R7364	T9194	T9192	arg1Of	differentiation,Th1
R7365	T9194	T9193	arg1Of	differentiation,cell
R7366	T9194	T9195	arg1Of	differentiation,was
R7367	T9194	T9196	arg2Of	differentiation,regulated
R7368	T9196	T9190	arg2Of	regulated,reported
R7369	T9196	T9191	arg1Of	regulated,that
R7370	T9196	T9195	arg2Of	regulated,was
R7371	T9196	T9197	arg1Of	regulated,through
R7372	T9200	T9197	arg2Of	loop,through
R7373	T9200	T9198	arg1Of	loop,a
R7374	T9200	T9199	arg1Of	loop,feed-forward
R7375	T9200	T9201	arg2Of	loop,in
R7376	T9200	T9202	arg1Of	loop,which
R7377	T9129	T9130	arg1Of	Fig.,3
R7378	T9203	T9204	arg1Of	T-bet,is
R7379	T9203	T9205	arg2Of	T-bet,up-regulated
R7380	T9129	T9131	arg1Of	Fig.,","
R7381	T9132	T9133	arg1Of	A,and
R7382	T9205	T9201	arg1Of	up-regulated,in
R7383	T9205	T9204	arg2Of	up-regulated,is
R7384	T9205	T9206	arg1Of	up-regulated,early
R7385	T9207	T9189	arg1Of	and,previously
R7386	T9208	T9207	arg2Of	induces,and
R7387	T9208	T9223	arg1Of	induces,","
R7388	T9208	T9225	modOf	induces,promoting
R7389	T9209	T9208	arg2Of	Runx3,induces
R7390	T9209	T9210	arg1Of	Runx3,","
R7391	T9209	T9211	arg2Of	Runx3,after
R7392	T9209	T9212	arg1Of	Runx3,which
R7393	T9133	T9131	arg2Of	and,","
R7394	T9213	T9214	arg1Of	T-bet,and
R7395	T9214	T9216	arg1Of	and,cooperate
R7396	T9214	T9218	arg1Of	and,induce
R7397	T9134	T9133	arg2Of	B,and
R7398	T9215	T9214	arg2Of	Runx3,and
R7399	T9216	T9211	arg1Of	cooperate,after
R7400	T9233	T9232	arg2Of	15,(
R7401	T9218	T9216	arg2Of	induce,cooperate
R7402	T9218	T9217	arg1Of	induce,to
R7403	T9219	T9220	arg1Of	IFN-γ,and
R7404	T9221	T9220	arg2Of	silence,and
R7405	T9222	T9218	arg2Of	IL-4,induce
R7406	T9222	T9219	arg1Of	IL-4,IFN-γ
R7407	T9222	T9221	arg1Of	IL-4,silence
R7408	T9233	T9234	arg1Of	15,","
R7409	T9235	T9234	arg2Of	22,","
R7410	T9225	T9224	arg1Of	promoting,thus
R7411	T9227	T9225	arg2Of	differentiation,promoting
R7412	T9227	T9226	arg1Of	differentiation,stable
R7413	T9227	T9228	arg1Of	differentiation,toward
R7414	T9227	T9232	arg1Of	differentiation,(
R7415	T9236	T9232	arg3Of	),(
R7416	T9241	T9239	arg1Of	Runx3,a
R7417	T9231	T9228	arg2Of	lineage,toward
R7418	T9231	T9229	arg1Of	lineage,the
R7419	T9231	T9230	arg1Of	lineage,Th1
R7420	T9241	T9240	arg1Of	Runx3,)
R7421	T9241	T9242	arg1Of	Runx3,appeared
R7422	T9241	T9243	arg1Of	Runx3,necessary
R7423	T9242	T9252	arg1Of	appeared,","
R7424	T9243	T9242	arg2Of	necessary,appeared
R7425	T9243	T9244	arg1Of	necessary,for
R7426	T9330	T9331	arg1Of	we,asked
R7427	T9246	T9244	arg2Of	induction,for
R7428	T9331	T9328	arg1Of	asked,Specifically
R7429	T9331	T9329	arg1Of	asked,","
R7430	T9333	T9334	arg1Of	Runx3,","
R7431	T9333	T9335	arg1Of	Runx3,which
R7432	T9333	T9336	arg1Of	Runx3,was
R7433	T9333	T9337	arg1Of	Runx3,necessary
R7434	T9246	T9245	arg1Of	induction,Eomes
R7435	T9333	T9343	arg1Of	Runx3,cooperated
R7436	T9246	T9247	arg1Of	induction,(
R7437	T9333	T9347	arg1Of	Runx3,regulate
R7438	T9250	T9247	arg2Of	A,(
R7439	T9337	T9336	arg2Of	necessary,was
R7440	T9337	T9338	arg1Of	necessary,for
R7441	T9250	T9248	arg1Of	A,Fig.
R7442	T9340	T9338	arg2Of	induction,for
R7443	T9340	T9339	arg1Of	induction,Eomes
R7444	T9250	T9249	arg1Of	A,3
R7445	T9251	T9247	arg3Of	),(
R7446	T9343	T9331	arg2Of	cooperated,asked
R7447	T9343	T9332	arg1Of	cooperated,whether
R7448	T9343	T9341	arg1Of	cooperated,","
R7449	T9343	T9342	arg1Of	cooperated,then
R7450	T9343	T9344	arg1Of	cooperated,with
R7451	T9343	T9346	modOf	cooperated,to
R7452	T9252	T9237	arg2Of	",",Because
R7453	T9345	T9344	arg2Of	Eomes,with
R7454	T9252	T9238	arg1Of	",",(
R7455	T9347	T9346	arg1Of	regulate,to
R7456	T9254	T9253	arg2Of	b,(
R7457	T9348	T9347	arg2Of	transcription,regulate
R7458	T9348	T9349	arg1Of	transcription,of
R7459	T9255	T9253	arg3Of	),(
R7460	T9354	T9355	arg1Of	perforin,","
R7461	T9355	T9358	arg1Of	",",and
R7462	T9257	T9256	arg1Of	kinetics,the
R7463	T9356	T9355	arg2Of	IFN-γ,","
R7464	T9358	T9349	arg2Of	and,of
R7465	T9358	T9350	arg1Of	and,the
R7466	T9358	T9351	arg1Of	and,effector
R7467	T9358	T9352	arg1Of	and,CTL
R7468	T9358	T9353	arg1Of	and,markers
R7469	T9358	T9357	arg1Of	and,","
R7470	T9360	T9358	arg2Of	B,and
R7471	T9360	T9359	arg1Of	B,granzyme
R7472	T9362	T9361	arg1Of	test,To
R7473	T9257	T9258	arg1Of	kinetics,of
R7474	T9364	T9362	arg2Of	hypothesis,test
R7475	T9364	T9363	arg1Of	hypothesis,this
R7476	T9366	T9362	arg1Of	we,test
R7477	T9366	T9367	arg1Of	we,used
R7478	T9366	T9375	arg1Of	we,ask
R7479	T9367	T9361	modOf	used,To
R7480	T9367	T9365	arg1Of	used,","
R7481	T9257	T9261	arg1Of	kinetics,paralleled
R7482	T9260	T9258	arg2Of	expression,of
R7483	T9371	T9370	arg2Of	ChIP,(
R7484	T9372	T9370	arg3Of	),(
R7485	T9373	T9367	arg2Of	assays,used
R7486	T9373	T9368	arg1Of	assays,chromatin
R7487	T9373	T9369	arg1Of	assays,immunoprecipitation
R7488	T9373	T9370	arg1Of	assays,(
R7489	T9260	T9259	arg1Of	expression,Eomes
R7490	T9375	T9367	arg3Of	ask,used
R7491	T9375	T9374	arg1Of	ask,to
R7492	T9261	T9271	arg1Of	paralleled,and
R7493	T9377	T9378	arg1Of	Eomes,and
R7494	T9378	T9380	arg1Of	and,bound
R7495	T9379	T9378	arg2Of	Runx3,and
R7496	T9380	T9375	arg2Of	bound,ask
R7497	T9380	T9376	arg1Of	bound,whether
R7498	T9262	T9261	arg2Of	those,paralleled
R7499	T9382	T9380	arg2Of	regions,bound
R7500	T9382	T9381	arg1Of	regions,regulatory
R7501	T9382	T9383	arg1Of	regions,of
R7502	T9262	T9263	arg1Of	those,of
R7503	T9265	T9263	arg2Of	expression,of
R7504	T9385	T9386	arg1Of	Prf1,","
R7505	T9386	T9389	arg1Of	",",and
R7506	T9387	T9386	arg2Of	Ifng,","
R7507	T9389	T9383	arg2Of	and,of
R7508	T9389	T9384	arg1Of	and,the
R7509	T9389	T9388	arg1Of	and,","
R7510	T9265	T9264	arg1Of	expression,perforin
R7511	T9391	T9389	arg2Of	genes,and
R7512	T9391	T9390	arg1Of	genes,Gzmb
R7513	T9391	T9392	arg1Of	genes,(
R7514	T9265	T9266	arg1Of	expression,(
R7515	T9267	T9266	arg2Of	Fig.,(
R7516	T9395	T9392	arg2Of	D,(
R7517	T9395	T9393	arg1Of	D,Fig.
R7518	T9395	T9394	arg1Of	D,3
R7519	T9396	T9392	arg3Of	),(
R7520	T9398	T9397	arg1Of	proteins,Both
R7521	T9398	T9399	arg2Of	proteins,associated
R7522	T9399	T9400	arg1Of	associated,with
R7523	T9267	T9268	arg1Of	Fig.,2
R7524	T9269	T9266	arg3Of	),(
R7525	T9403	T9400	arg2Of	regions,with
R7526	T9403	T9401	arg1Of	regions,gene
R7527	T9403	T9402	arg1Of	regions,regulatory
R7528	T9403	T9404	arg1Of	regions,in
R7529	T9406	T9404	arg2Of	CTLs,in
R7530	T9406	T9405	arg2Of	CTLs,differentiated
R7531	T9407	T9408	arg1Of	Runx3,bound
R7532	T9271	T9252	arg2Of	and,","
R7533	T9408	T9409	arg1Of	bound,to
R7534	T9271	T9253	arg1Of	and,(
R7535	T9408	T9420	arg1Of	bound,;
R7536	T9408	T9421	arg1Of	bound,to
R7537	T9408	T9438	arg1Of	bound,;
R7538	T9408	T9439	arg1Of	bound,to
R7539	T9408	T9455	arg1Of	bound,;
R7540	T9408	T9456	arg1Of	bound,to
R7541	T9271	T9270	arg1Of	and,","
R7542	T9273	T9272	arg2Of	c,(
R7543	T9411	T9412	arg1Of	Prf1,and
R7544	T9413	T9412	arg2Of	Gzmb,and
R7545	T9274	T9272	arg3Of	),(
R7546	T9416	T9409	arg2Of	sites,to
R7547	T9416	T9410	arg1Of	sites,the
R7548	T9416	T9411	arg1Of	sites,Prf1
R7549	T9416	T9413	arg1Of	sites,Gzmb
R7550	T9416	T9414	arg1Of	sites,transcription
R7551	T9416	T9415	arg1Of	sites,start
R7552	T9416	T9417	arg1Of	sites,(
R7553	T9418	T9417	arg2Of	TSS,(
R7554	T9275	T9272	arg1Of	overexpression,(
R7555	T9419	T9417	arg3Of	),(
R7556	T9275	T9276	arg1Of	overexpression,of
R7557	T9275	T9278	arg1Of	overexpression,in
R7558	T9275	T9285	arg1Of	overexpression,led
R7559	T9277	T9276	arg2Of	Eo-VP16,of
R7560	T9280	T9281	arg1Of	WT,or
R7561	T9281	T9278	arg2Of	or,in
R7562	T9426	T9421	arg2Of	enhancer,to
R7563	T9426	T9422	arg1Of	enhancer,a
R7564	T9426	T9423	arg2Of	enhancer,known
R7565	T9426	T9424	arg1Of	enhancer,IL-2
R7566	T9426	T9425	arg1Of	enhancer,responsive
R7567	T9426	T9427	arg1Of	enhancer,located
R7568	T9427	T9428	arg1Of	located,near
R7569	T9281	T9279	arg1Of	or,either
R7570	T9430	T9428	arg2Of	kb,near
R7571	T9430	T9429	arg1Of	kb,−1
R7572	T9430	T9431	arg1Of	kb,of
R7573	T9284	T9281	arg2Of	cells,or
R7574	T9434	T9431	arg2Of	gene,of
R7575	T9434	T9432	arg1Of	gene,the
R7576	T9434	T9433	arg1Of	gene,Prf1
R7577	T9434	T9435	arg1Of	gene,(
R7578	T9436	T9435	arg2Of	23,(
R7579	T9437	T9435	arg3Of	),(
R7580	T9284	T9282	arg1Of	cells,T-bet–deficient
R7581	T9284	T9283	arg1Of	cells,T
R7582	T9285	T9271	arg2Of	led,and
R7583	T9285	T9286	arg1Of	led,to
R7584	T9288	T9286	arg2Of	increase,to
R7585	T9446	T9439	arg2Of	site,to
R7586	T9446	T9440	arg1Of	site,the
R7587	T9446	T9441	arg1Of	site,distal
R7588	T9446	T9442	arg1Of	site,CTL-specific
R7589	T9446	T9443	arg1Of	site,DNase
R7590	T9446	T9444	arg1Of	site,I
R7591	T9446	T9445	arg1Of	site,hypersensitive
R7592	T9446	T9447	arg1Of	site,9
R7593	T9446	T9448	arg1Of	site,in
R7594	T9451	T9448	arg2Of	locus,in
R7595	T9451	T9449	arg1Of	locus,the
R7596	T9451	T9450	arg1Of	locus,Prf1
R7597	T9451	T9452	arg1Of	locus,(
R7598	T9288	T9287	arg1Of	increase,an
R7599	T9453	T9452	arg2Of	24,(
R7600	T9288	T9289	arg1Of	increase,in
R7601	T9454	T9452	arg3Of	),(
R7602	T9291	T9292	arg1Of	perforin,and
R7603	T9459	T9456	arg2Of	promoter,to
R7604	T9459	T9457	arg1Of	promoter,the
R7605	T9459	T9458	arg1Of	promoter,Ifng
R7606	T9459	T9460	arg1Of	promoter,near
R7607	T9459	T9466	arg2Of	promoter,reported
R7608	T9292	T9290	arg1Of	and,both
R7609	T9462	T9460	arg2Of	TSS,near
R7610	T9462	T9461	arg1Of	TSS,the
R7611	T9293	T9292	arg2Of	IFN-γ,and
R7612	T9465	T9464	arg1Of	previously,as
R7613	T9466	T9463	arg1Of	reported,","
R7614	T9466	T9465	arg1Of	reported,previously
R7615	T9466	T9467	arg1Of	reported,for
R7616	T9466	T9475	arg1Of	reported,to
R7617	T9467	T9474	arg1Of	for,and
R7618	T9469	T9467	arg2Of	cells,for
R7619	T9469	T9468	arg1Of	cells,Th1
R7620	T9469	T9470	arg1Of	cells,(
R7621	T9294	T9289	arg2Of	expression,in
R7622	T9471	T9470	arg2Of	10,(
R7623	T9472	T9470	arg3Of	),(
R7624	T9294	T9291	arg1Of	expression,perforin
R7625	T9474	T9473	arg1Of	and,;
R7626	T9475	T9474	arg2Of	to,and
R7627	T9294	T9293	arg1Of	expression,IFN-γ
R7628	T9294	T9295	arg1Of	expression,(
R7629	T9296	T9297	arg1Of	Fig.,2
R7630	T9480	T9475	arg2Of	sites,to
R7631	T9480	T9476	arg1Of	sites,several
R7632	T9480	T9477	arg1Of	sites,DNase
R7633	T9480	T9478	arg1Of	sites,I
R7634	T9480	T9479	arg1Of	sites,hypersensitive
R7635	T9480	T9481	arg1Of	sites,in
R7636	T9296	T9298	arg1Of	Fig.,","
R7637	T9480	T9493	arg1Of	sites,(
R7638	T9298	T9300	arg1Of	",",and
R7639	T9299	T9298	arg2Of	B,","
R7640	T9484	T9481	arg2Of	locus,in
R7641	T9484	T9482	arg1Of	locus,the
R7642	T9484	T9483	arg1Of	locus,Ifng
R7643	T9484	T9485	arg1Of	locus,(
R7644	T9300	T9295	arg2Of	and,(
R7645	T9488	T9485	arg2Of	D,(
R7646	T9488	T9486	arg1Of	D,Fig.
R7647	T9488	T9487	arg1Of	D,3
R7648	T9488	T9491	arg1Of	D,depicted
R7649	T9491	T9489	arg1Of	depicted,and
R7650	T9491	T9490	arg1Of	depicted,not
R7651	T9301	T9300	arg2Of	D,and
R7652	T9492	T9485	arg3Of	),(
R7653	T9494	T9493	arg2Of	25,(
R7654	T9302	T9295	arg3Of	),(
R7655	T9495	T9493	arg3Of	),(
R7656	T9496	T9497	arg1Of	Eomes,bound
R7657	T9304	T9305	arg1Of	we,asked
R7658	T9497	T9499	arg1Of	bound,to
R7659	T9497	T9508	arg1Of	bound,;
R7660	T9499	T9498	arg1Of	to,primarily
R7661	T9305	T9237	arg1Of	asked,Because
R7662	T9502	T9500	arg1Of	TSS,the
R7663	T9502	T9501	arg1Of	TSS,Prf1
R7664	T9502	T9503	arg1Of	TSS,and
R7665	T9503	T9499	arg2Of	and,to
R7666	T9305	T9303	arg1Of	asked,","
R7667	T9308	T9307	arg1Of	differentiation,CTL
R7668	T9507	T9503	arg2Of	enhancer,and
R7669	T9507	T9504	arg1Of	enhancer,the
R7670	T9507	T9505	arg1Of	enhancer,−1
R7671	T9507	T9506	arg1Of	enhancer,kb
R7672	T9510	T9509	arg1Of	binding,this
R7673	T9510	T9511	arg1Of	binding,was
R7674	T9510	T9513	arg1Of	binding,greater
R7675	T9510	T9536	arg1Of	binding,comparable
R7676	T9511	T9508	arg2Of	was,;
R7677	T9513	T9512	arg1Of	greater,substantially
R7678	T9513	T9514	arg1Of	greater,than
R7679	T9513	T9535	arg1Of	greater,and
R7680	T9515	T9514	arg2Of	that,than
R7681	T9515	T9516	arg2Of	that,observed
R7682	T9515	T9524	arg1Of	that,","
R7683	T9308	T9309	arg1Of	differentiation,was
R7684	T9516	T9517	arg1Of	observed,at
R7685	T9308	T9312	arg2Of	differentiation,regulated
R7686	T9312	T9305	arg2Of	regulated,asked
R7687	T9519	T9517	arg2Of	promoter,at
R7688	T9519	T9518	arg1Of	promoter,the
R7689	T9519	T9520	arg1Of	promoter,of
R7690	T9312	T9306	arg1Of	regulated,whether
R7691	T9312	T9309	arg2Of	regulated,was
R7692	T9312	T9310	arg1Of	regulated,also
R7693	T9312	T9311	arg1Of	regulated,potentially
R7694	T9316	T9312	arg1Of	loop,regulated
R7695	T9523	T9520	arg2Of	gene,of
R7696	T9523	T9521	arg1Of	gene,the
R7697	T9523	T9522	arg1Of	gene,Il2rb
R7698	T9316	T9313	arg2Of	loop,by
R7699	T9316	T9314	arg1Of	loop,a
R7700	T9528	T9524	arg2Of	target,","
R7701	T9528	T9525	arg1Of	target,a
R7702	T9528	T9526	arg1Of	target,known
R7703	T9528	T9527	arg1Of	target,direct
R7704	T9528	T9529	arg1Of	target,of
R7705	T9316	T9315	arg1Of	loop,feed-forward
R7706	T9530	T9529	arg2Of	Eomes,of
R7707	T9530	T9531	arg1Of	Eomes,(
R7708	T9316	T9317	arg1Of	loop,involving
R7709	T9532	T9531	arg2Of	8,(
R7710	T9321	T9317	arg2Of	classes,involving
R7711	T9533	T9531	arg3Of	),(
R7712	T9535	T9511	arg2Of	and,was
R7713	T9535	T9534	arg1Of	and,","
R7714	T9536	T9535	arg2Of	comparable,and
R7715	T9536	T9537	arg1Of	comparable,to
R7716	T9538	T9537	arg2Of	that,to
R7717	T9538	T9539	arg2Of	that,observed
R7718	T9538	T9544	arg1Of	that,","
R7719	T9539	T9540	arg1Of	observed,at
R7720	T9321	T9318	arg1Of	classes,these
R7721	T9321	T9319	arg1Of	classes,same
R7722	T9543	T9540	arg2Of	TSS,at
R7723	T9543	T9541	arg1Of	TSS,the
R7724	T9543	T9542	arg1Of	TSS,Ifng
R7725	T9321	T9320	arg1Of	classes,two
R7726	T9321	T9322	arg1Of	classes,of
R7727	T9547	T9544	arg2Of	target,","
R7728	T9547	T9545	arg1Of	target,a
R7729	T9547	T9546	arg2Of	target,known
R7730	T9547	T9548	arg1Of	target,of
R7731	T9547	T9551	arg1Of	target,in
R7732	T9323	T9324	arg1Of	Runx,and
R7733	T9547	T9563	arg1Of	target,(
R7734	T9550	T9548	arg2Of	proteins,of
R7735	T9550	T9549	arg1Of	proteins,T-box
R7736	T9325	T9324	arg2Of	T-box,and
R7737	T9327	T9322	arg2Of	factors,of
R7738	T9557	T9551	arg2Of	cells,in
R7739	T9557	T9552	arg1Of	cells,both
R7740	T9557	T9553	arg1Of	cells,Th1
R7741	T9557	T9554	arg1Of	cells,and
R7742	T9557	T9555	arg1Of	cells,CD8+
R7743	T9557	T9556	arg1Of	cells,T
R7744	T9557	T9558	arg1Of	cells,(
R7745	T9327	T9323	arg1Of	factors,Runx
R7746	T9327	T9325	arg1Of	factors,T-box
R7747	T9561	T9558	arg2Of	D,(
R7748	T9561	T9559	arg1Of	D,Fig.
R7749	T9561	T9560	arg1Of	D,3
R7750	T9562	T9558	arg3Of	),(
R7751	T9327	T9326	arg1Of	factors,transcription
R7752	T9622	T9619	arg2Of	cells,and
R7753	T9564	T9563	arg2Of	17,(
R7754	T9565	T9563	arg3Of	),(
R7755	T9567	T9566	arg1Of	determine,To
R7756	T9569	T9570	arg1Of	Runx3,controlled
R7757	T9570	T9567	arg2Of	controlled,determine
R7758	T9570	T9568	arg1Of	controlled,whether
R7759	T9570	T9577	arg1Of	controlled,through
R7760	T9622	T9620	arg1Of	cells,CD8+CD4+
R7761	T9572	T9570	arg2Of	expression,controlled
R7762	T9572	T9571	arg1Of	expression,the
R7763	T9572	T9573	arg1Of	expression,of
R7764	T9622	T9621	arg1Of	cells,DP
R7765	T9626	T9623	arg2Of	experiments,in
R7766	T9576	T9573	arg2Of	genes,of
R7767	T9576	T9574	arg1Of	genes,CTL
R7768	T9576	T9575	arg1Of	genes,effector
R7769	T9579	T9577	arg2Of	induction,through
R7770	T9579	T9578	arg1Of	induction,its
R7771	T9579	T9580	arg1Of	induction,of
R7772	T9581	T9580	arg2Of	Eomes,of
R7773	T9583	T9567	arg1Of	we,determine
R7774	T9583	T9585	arg1Of	we,expressed
R7775	T9585	T9566	modOf	expressed,To
R7776	T9585	T9582	arg1Of	expressed,","
R7777	T9585	T9584	arg1Of	expressed,retrovirally
R7778	T9585	T9589	arg1Of	expressed,in
R7779	T9586	T9587	arg1Of	Runx3,and
R7780	T9626	T9624	arg1Of	experiments,our
R7781	T9587	T9585	arg2Of	and,expressed
R7782	T9626	T9625	arg1Of	experiments,previous
R7783	T9588	T9587	arg2Of	Eo-VP16,and
R7784	T9628	T9629	arg1Of	we,used
R7785	T9592	T9589	arg2Of	cells,in
R7786	T9592	T9590	arg1Of	cells,CD8+
R7787	T9592	T9591	arg1Of	cells,T
R7788	T9592	T9593	arg1Of	cells,from
R7789	T9629	T9596	arg1Of	used,Because
R7790	T9595	T9593	arg2Of	mice,from
R7791	T9595	T9594	arg1Of	mice,Runx3−/−
R7792	T9596	T9597	arg1Of	Because,of
R7793	T9596	T9609	arg1Of	Because,and
R7794	T9629	T9610	arg1Of	used,because
R7795	T9629	T9627	arg1Of	used,","
R7796	T9600	T9596	arg2Of	number,Because
R7797	T9600	T9598	arg1Of	number,the
R7798	T9600	T9599	arg1Of	number,limited
R7799	T9600	T9601	arg1Of	number,of
R7800	T9600	T9605	arg1Of	number,in
R7801	T9629	T9635	arg1Of	used,without
R7802	T9629	T9638	arg1Of	used,as
R7803	T9604	T9601	arg2Of	cells,of
R7804	T9604	T9602	arg1Of	cells,CD8+
R7805	T9604	T9603	arg1Of	cells,T
R7806	T9634	T9629	arg2Of	cells,used
R7807	T9607	T9605	arg2Of	mice,in
R7808	T9607	T9606	arg1Of	mice,these
R7809	T9609	T9608	arg1Of	and,","
R7810	T9610	T9609	arg2Of	because,and
R7811	T9611	T9612	arg1Of	we,saw
R7812	T9612	T9610	arg2Of	saw,because
R7813	T9612	T9623	arg1Of	saw,in
R7814	T9634	T9630	arg1Of	cells,total
R7815	T9614	T9612	arg2Of	difference,saw
R7816	T9634	T9631	arg1Of	cells,Runx3−/−
R7817	T9634	T9632	arg1Of	cells,CD8+
R7818	T9634	T9633	arg1Of	cells,T
R7819	T9637	T9635	arg2Of	fractionation,without
R7820	T9614	T9613	arg1Of	difference,no
R7821	T9614	T9615	arg1Of	difference,between
R7822	T9618	T9616	arg1Of	SP,Runx3−/−
R7823	T9618	T9617	arg1Of	SP,CD8+CD4−
R7824	T9618	T9619	arg1Of	SP,and
R7825	T9619	T9615	arg2Of	and,between
R7826	T9637	T9636	arg1Of	fractionation,further
R7827	T9639	T9638	arg2Of	recipients,as
R7828	T9639	T9640	arg1Of	recipients,for
R7829	T9642	T9640	arg2Of	transduction,for
R7830	T9719	T9718	arg1Of	though,even
R7831	T9642	T9641	arg1Of	transduction,retroviral
R7832	T9643	T9644	arg1Of	Reconstitution,of
R7833	T9720	T9721	arg1Of	it,restored
R7834	T9721	T9719	arg2Of	restored,though
R7835	T9643	T9649	arg1Of	Reconstitution,with
R7836	T9643	T9651	arg1Of	Reconstitution,restored
R7837	T9723	T9721	arg2Of	capacity,restored
R7838	T9723	T9722	arg1Of	capacity,the
R7839	T9723	T9724	modOf	capacity,to
R7840	T9648	T9644	arg2Of	cells,of
R7841	T9725	T9724	arg1Of	induce,to
R7842	T9725	T9728	arg1Of	induce,upon
R7843	T9727	T9725	arg2Of	expression,induce
R7844	T9727	T9726	arg1Of	expression,IFN-γ
R7845	T9730	T9728	arg2Of	restimulation,upon
R7846	T9730	T9729	arg1Of	restimulation,TCR
R7847	T9730	T9731	arg1Of	restimulation,(
R7848	T9732	T9731	arg2Of	Fig.,(
R7849	T9732	T9733	arg1Of	Fig.,4
R7850	T9732	T9734	arg1Of	Fig.,","
R7851	T9648	T9645	arg1Of	cells,Runx3−/−
R7852	T9648	T9646	arg1Of	cells,CD8+
R7853	T9735	T9736	arg1Of	A,and
R7854	T9648	T9647	arg1Of	cells,T
R7855	T9736	T9734	arg2Of	and,","
R7856	T9650	T9649	arg2Of	Runx3,with
R7857	T9737	T9736	arg2Of	B,and
R7858	T9738	T9731	arg3Of	),(
R7859	T9740	T9739	arg1Of	result,This
R7860	T9740	T9741	arg1Of	result,suggests
R7861	T9741	T9742	arg1Of	suggests,strongly
R7862	T9652	T9651	arg2Of	expression,restored
R7863	T9652	T9653	arg1Of	expression,of
R7864	T9745	T9744	arg1Of	expression,perforin
R7865	T9745	T9746	arg1Of	expression,requires
R7866	T9746	T9741	arg2Of	requires,suggests
R7867	T9746	T9743	arg1Of	requires,that
R7868	T9652	T9665	arg1Of	expression,(
R7869	T9747	T9748	arg1Of	Runx3,and
R7870	T9748	T9746	arg2Of	and,requires
R7871	T9749	T9748	arg2Of	Eomes,and
R7872	T9654	T9657	arg1Of	Eomes,as
R7873	T9751	T9750	arg2Of	expected,As
R7874	T9751	T9752	arg1Of	expected,from
R7875	T9657	T9653	arg2Of	as,of
R7876	T9754	T9752	arg2Of	defect,from
R7877	T9754	T9753	arg1Of	defect,their
R7878	T9754	T9755	arg1Of	defect,in
R7879	T9756	T9757	arg1Of	perforin,and
R7880	T9758	T9757	arg2Of	granzyme,and
R7881	T9760	T9755	arg2Of	expression,in
R7882	T9760	T9756	arg1Of	expression,perforin
R7883	T9760	T9758	arg1Of	expression,granzyme
R7884	T9760	T9759	arg1Of	expression,B
R7885	T9657	T9655	arg1Of	as,as
R7886	T9657	T9656	arg1Of	as,well
R7887	T9658	T9659	arg1Of	perforin,","
R7888	T9765	T9762	arg1Of	cells,Runx3−/−
R7889	T9765	T9763	arg1Of	cells,CD8+
R7890	T9765	T9764	arg1Of	cells,T
R7891	T9765	T9766	arg1Of	cells,showed
R7892	T9766	T9750	arg1Of	showed,As
R7893	T9766	T9761	arg1Of	showed,","
R7894	T9766	T9770	arg1Of	showed,in
R7895	T9768	T9767	arg1Of	cytolytic,defective
R7896	T9659	T9663	arg1Of	",",and
R7897	T9769	T9766	arg2Of	activity,showed
R7898	T9769	T9768	arg1Of	activity,cytolytic
R7899	T9661	T9659	arg2Of	B,","
R7900	T9774	T9770	arg2Of	reaction,in
R7901	T9774	T9771	arg1Of	reaction,a
R7902	T9774	T9772	arg1Of	reaction,mixed
R7903	T9774	T9773	arg1Of	reaction,lymphocyte
R7904	T9774	T9775	arg1Of	reaction,(
R7905	T9776	T9775	arg2Of	12,(
R7906	T9661	T9660	arg1Of	B,granzyme
R7907	T9777	T9775	arg3Of	),(
R7908	T9663	T9657	arg2Of	and,as
R7909	T9663	T9662	arg1Of	and,","
R7910	T9664	T9663	arg2Of	IFN-γ,and
R7911	T9783	T9780	arg1Of	cells,TCR-stimulated
R7912	T9783	T9781	arg1Of	cells,Runx3−/−
R7913	T9783	T9782	arg1Of	cells,CD8+
R7914	T9783	T9784	arg1Of	cells,were
R7915	T9783	T9786	arg1Of	cells,effective
R7916	T9784	T9778	arg1Of	were,However
R7917	T9784	T9779	arg1Of	were,","
R7918	T9786	T9784	arg2Of	effective,were
R7919	T9786	T9785	arg1Of	effective,as
R7920	T9786	T9787	arg1Of	effective,as
R7921	T9789	T9787	arg2Of	cells,as
R7922	T9666	T9665	arg2Of	Fig.,(
R7923	T9789	T9788	arg1Of	cells,WT
R7924	T9789	T9790	arg1Of	cells,in
R7925	T9666	T9667	arg1Of	Fig.,4
R7926	T9791	T9790	arg2Of	killing,in
R7927	T9791	T9794	arg1Of	killing,in
R7928	T9666	T9668	arg1Of	Fig.,","
R7929	T9669	T9670	arg1Of	A,and
R7930	T9793	T9791	arg2Of	cells,killing
R7931	T9793	T9792	arg1Of	cells,tumor
R7932	T9670	T9668	arg2Of	and,","
R7933	T9798	T9794	arg2Of	assay,in
R7934	T9798	T9795	arg1Of	assay,a
R7935	T9798	T9796	arg1Of	assay,redirected
R7936	T9798	T9797	arg1Of	assay,CTL
R7937	T9798	T9799	arg1Of	assay,(
R7938	T9800	T9799	arg2Of	12,(
R7939	T9671	T9670	arg2Of	B,and
R7940	T9801	T9799	arg3Of	),(
R7941	T9672	T9665	arg3Of	),(
R7942	T9805	T9804	arg1Of	cells,CD8+
R7943	T9805	T9806	arg1Of	cells,from
R7944	T9805	T9813	arg2Of	cells,immunized
R7945	T9805	T9819	arg1Of	cells,killed
R7946	T9674	T9673	arg2Of	addition,In
R7947	T9809	T9806	arg2Of	cavity,from
R7948	T9809	T9807	arg1Of	cavity,the
R7949	T9809	T9808	arg1Of	cavity,peritoneal
R7950	T9809	T9810	arg1Of	cavity,of
R7951	T9678	T9676	arg1Of	cells,Runx3−/−
R7952	T9812	T9810	arg2Of	mice,of
R7953	T9812	T9811	arg1Of	mice,Runx3−/−
R7954	T9813	T9814	arg1Of	immunized,with
R7955	T9678	T9677	arg1Of	cells,T
R7956	T9678	T9679	arg1Of	cells,showed
R7957	T9679	T9673	arg1Of	showed,In
R7958	T9679	T9675	arg1Of	showed,","
R7959	T9679	T9693	arg1Of	showed,","
R7960	T9679	T9694	modOf	showed,indicating
R7961	T9817	T9814	arg2Of	cells,with
R7962	T9817	T9815	arg1Of	cells,certain
R7963	T9817	T9816	arg1Of	cells,tumor
R7964	T9682	T9679	arg2Of	up-regulation,showed
R7965	T9819	T9802	arg1Of	killed,Furthermore
R7966	T9819	T9803	arg1Of	killed,","
R7967	T9819	T9818	arg1Of	killed,effectively
R7968	T9821	T9819	arg2Of	targets,killed
R7969	T9821	T9820	arg1Of	targets,these
R7970	T9821	T9822	arg1Of	targets,(
R7971	T9823	T9822	arg2Of	13,(
R7972	T9824	T9822	arg3Of	),(
R7973	T9682	T9680	arg1Of	up-regulation,a
R7974	T9682	T9681	arg1Of	up-regulation,compensatory
R7975	T9828	T9829	arg1Of	activation,of
R7976	T9682	T9683	arg1Of	up-regulation,of
R7977	T9828	T9834	arg1Of	activation,is
R7978	T9828	T9835	arg1Of	activation,defective
R7979	T9682	T9685	arg1Of	up-regulation,","
R7980	T9682	T9686	arg1Of	up-regulation,which
R7981	T9682	T9687	arg1Of	up-regulation,was
R7982	T9833	T9829	arg2Of	machinery,of
R7983	T9833	T9830	arg1Of	machinery,the
R7984	T9833	T9831	arg1Of	machinery,perforin/granzyme
R7985	T9833	T9832	arg1Of	machinery,B
R7986	T9834	T9827	arg2Of	is,although
R7987	T9682	T9688	arg2Of	up-regulation,suppressed
R7988	T9835	T9834	arg2Of	defective,is
R7989	T9835	T9836	arg1Of	defective,in
R7990	T9684	T9683	arg2Of	Runx1,of
R7991	T9839	T9836	arg2Of	cells,in
R7992	T9839	T9837	arg1Of	cells,Runx3−/−
R7993	T9839	T9838	arg1Of	cells,CD8+
R7994	T9688	T9687	arg2Of	suppressed,was
R7995	T9842	T9841	arg1Of	cells,these
R7996	T9842	T9843	arg1Of	cells,are
R7997	T9842	T9846	arg1Of	cells,devoid
R7998	T9842	T9851	arg1Of	cells,could
R7999	T9842	T9854	arg1Of	cells,kill
R8000	T9843	T9844	arg1Of	are,not
R8001	T9843	T9850	arg1Of	are,and
R8002	T9846	T9843	arg2Of	devoid,are
R8003	T9846	T9845	arg1Of	devoid,entirely
R8004	T9846	T9847	arg1Of	devoid,of
R8005	T9688	T9689	arg1Of	suppressed,upon
R8006	T9849	T9847	arg2Of	activity,of
R8007	T9849	T9848	arg1Of	activity,cytolytic
R8008	T9850	T9825	arg1Of	and,Therefore
R8009	T9850	T9826	arg1Of	and,","
R8010	T9850	T9827	arg1Of	and,although
R8011	T9850	T9840	arg1Of	and,","
R8012	T9850	T9856	arg1Of	and,","
R8013	T9850	T9858	arg1Of	and,by
R8014	T9690	T9689	arg2Of	reconstitution,upon
R8015	T9854	T9850	arg2Of	kill,and
R8016	T9854	T9851	arg2Of	kill,could
R8017	T9854	T9852	arg1Of	kill,still
R8018	T9854	T9853	arg1Of	kill,effectively
R8019	T9855	T9854	arg2Of	targets,kill
R8020	T9690	T9691	arg1Of	reconstitution,with
R8021	T9858	T9857	arg1Of	by,possibly
R8022	T9860	T9858	arg2Of	mechanisms,by
R8023	T9860	T9859	arg1Of	mechanisms,alternative
R8024	T9692	T9691	arg2Of	Runx3,with
R8025	T9860	T9862	arg1Of	mechanisms,as
R8026	T9696	T9697	arg1Of	Runx1,is
R8027	T9862	T9861	arg1Of	as,such
R8028	T9866	T9862	arg2Of	pathway,as
R8029	T9866	T9863	arg1Of	pathway,the
R8030	T9866	T9864	arg1Of	pathway,Fas–Fas
R8031	T9866	T9865	arg1Of	pathway,ligand
R8032	T9697	T9694	arg2Of	is,indicating
R8033	T9697	T9695	arg1Of	is,that
R8034	T9699	T9697	arg2Of	target,is
R8035	T9699	T9698	arg1Of	target,a
R8036	T9699	T9700	arg1Of	target,of
R8037	T9699	T9702	arg1Of	target,by
R8038	T9701	T9700	arg2Of	repression,of
R8039	T9703	T9702	arg2Of	Runx3,by
R8040	T9706	T9707	arg1Of	Eo-VP16,did
R8043	T9706	T9709	arg1Of	Eo-VP16,up-regulate
R8044	T9709	T9704	arg1Of	up-regulate,Notably
R8047	T9709	T9705	arg1Of	up-regulate,","
R8050	T9709	T9707	arg2Of	up-regulate,did
R8051	T9709	T9708	arg1Of	up-regulate,not
R8052	T9709	T9712	arg1Of	up-regulate,when
R8053	T9709	T9717	arg1Of	up-regulate,","
R8054	T9709	T9719	arg1Of	up-regulate,though
R8055	T9711	T9709	arg2Of	expression,up-regulate
R8056	T9711	T9710	arg1Of	expression,perforin
R8057	T9713	T9712	arg2Of	expressed,when
R8058	T9713	T9714	arg1Of	expressed,in
R8059	T9716	T9714	arg2Of	cells,in
R8060	T9716	T9715	arg1Of	cells,Runx3−/−

PMC:2626671 JSONTXT 24 Projects

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PMC:2626671 JSON TXT 24 Projects