PMC:1942070 / 3700-4529 JSONTXT 19 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1675 36-43 NN denotes culture
T1676 43-44 -COMMA- denotes ,
T1677 45-54 JJ denotes transient
T1678 55-68 NN denotes transfections
T1679 69-72 CC denotes and
T1680 73-77 NN denotes cell
T1681 78-89 NN denotes stimulation
T1682 90-93 DT denotes The
T1683 94-104 NN denotes generation
T1684 104-105 -COMMA- denotes ,
T1685 106-113 NN denotes culture
T1686 114-117 CC denotes and
T1687 118-128 NN denotes activation
T1688 129-131 IN denotes of
T1689 132-139 NN denotes PKD1−/−
T1690 139-140 -COMMA- denotes ,
T1691 141-148 NN denotes PKD3−/−
T1692 149-152 CC denotes and
T1693 153-162 NN denotes PKD1/3−/−
T1694 163-171 NN denotes knockout
T1695 172-176 NN denotes DT40
T1696 177-178 NN denotes B
T1697 179-183 NN denotes cell
T1698 184-189 NN denotes lines
T1699 190-194 VB denotes have
T1700 195-199 VB denotes been
T1701 200-209 VB denotes described
T1702 210-220 RB denotes previously
T1703 221-222 -LRB- denotes [
T1704 222-223 CD denotes 1
T1705 223-224 -RRB- denotes ]
T1706 226-231 NN denotes Cells
T1707 232-236 VB denotes were
T1708 237-242 VB denotes lysed
T1709 243-246 CC denotes and
T1710 247-254 NN denotes protein
T1711 255-263 NN denotes extracts
T1712 264-268 VB denotes were
T1713 269-277 VB denotes analysed
T1714 278-280 IN denotes in
T1715 281-288 JJ denotes Western
T1716 289-297 NN denotes blotting
T1717 298-309 NN denotes experiments
T1718 310-312 IN denotes as
T1719 313-323 RB denotes previously
T1720 324-333 VB denotes described
T1721 334-335 -LRB- denotes [
T1722 335-336 CD denotes 1
T1723 336-337 -RRB- denotes ]
T1724 339-354 NN denotes Chloramphenicol
T1725 355-361 NN denotes acetyl
T1726 362-373 NN denotes transferase
T1727 374-380 NN denotes assays
T1728 381-385 VB denotes have
T1729 386-390 VB denotes been
T1730 391-400 VB denotes described
T1731 401-411 RB denotes previously
T1732 412-413 -LRB- denotes [
T1733 413-415 CD denotes 29
T1734 415-416 -RRB- denotes ]
T1869 429-437 NN denotes staining
T1870 438-442 NN denotes DT40
T1871 443-444 NN denotes B
T1872 445-450 NN denotes cells
T1873 451-452 -LRB- denotes (
T1874 452-459 CD denotes 2 × 106
T1875 460-465 NN denotes cells
T1876 466-469 IN denotes per
T1877 470-475 NN denotes point
T1878 475-476 -RRB- denotes )
T1879 477-481 VB denotes were
T1880 482-493 VB denotes resuspended
T1881 494-496 IN denotes in
T1882 497-503 NN denotes 200 μl
T1883 504-510 NN denotes buffer
T1884 511-512 -LRB- denotes (
T1885 512-516 NN denotes RPMI
T1886 517-521 CD denotes 1640
T1887 522-527 NN denotes media
T1888 527-528 -COMMA- denotes ,
T1889 529-530 CD denotes 1
T1890 530-531 NN denotes %
T1891 532-538 JJ denotes foetal
T1892 539-543 NN denotes calf
T1893 544-549 NN denotes serum
T1894 549-550 -RRB- denotes )
T1895 551-561 VB denotes containing
T1896 562-574 JJ denotes anti-chicken
T1897 575-577 NN denotes M1
T1898 578-588 JJ denotes monoclonal
T1899 589-597 NN denotes antibody
T1900 598-608 VB denotes conjugated
T1901 609-611 TO denotes to
T1902 612-616 NN denotes FITC
T1903 617-620 IN denotes for
T1904 621-627 NN denotes 20 min
T1905 628-630 IN denotes on
T1906 631-634 NN denotes ice
T1907 636-639 DT denotes The
T1908 640-645 NN denotes cells
T1909 646-650 VB denotes were
T1910 651-657 VB denotes washed
T1911 658-663 RB denotes twice
T1912 664-667 CC denotes and
T1913 668-679 JJ denotes fluorescent
T1914 680-689 NN denotes intensity
T1915 690-693 VB denotes was
T1916 694-702 VB denotes analysed
T1917 703-705 IN denotes by
T1918 706-710 NN denotes flow
T1919 711-720 NN denotes cytometry
T1920 722-725 DT denotes All
T1921 726-733 NN denotes results
T1922 734-739 VB denotes shown
T1923 740-743 VB denotes are
T1924 744-758 JJ denotes representative
T1925 759-761 IN denotes of
T1926 762-764 IN denotes at
T1927 765-768 CD denotes two
T1928 769-771 TO denotes to
T1929 772-776 CD denotes four
T1930 777-788 JJ denotes independent
T1931 789-800 NN denotes experiments
T1932 801-807 IN denotes unless
T1933 808-817 RB denotes otherwise
T1934 818-827 VB denotes indicated
R1438 T1675 T1676 arg1Of culture,","
R1439 T1676 T1679 arg1Of ",",and
R1440 T1678 T1676 arg2Of transfections,","
R1441 T1678 T1677 arg1Of transfections,transient
R1442 T1681 T1679 arg2Of stimulation,and
R1443 T1681 T1680 arg1Of stimulation,cell
R1444 T1683 T1684 arg1Of generation,","
R1445 T1684 T1686 arg1Of ",",and
R1446 T1685 T1684 arg2Of culture,","
R1447 T1686 T1682 arg1Of and,The
R1448 T1686 T1688 arg1Of and,of
R1449 T1686 T1699 arg1Of and,have
R1450 T1686 T1700 arg1Of and,been
R1451 T1686 T1701 arg2Of and,described
R1452 T1687 T1686 arg2Of activation,and
R1453 T1689 T1690 arg1Of PKD1−/−,","
R1454 T1690 T1692 arg1Of ",",and
R1455 T1691 T1690 arg2Of PKD3−/−,","
R1456 T1693 T1692 arg2Of PKD1/3−/−,and
R1457 T1698 T1688 arg2Of lines,of
R1458 T1698 T1689 arg1Of lines,PKD1−/−
R1459 T1698 T1691 arg1Of lines,PKD3−/−
R1460 T1698 T1693 arg1Of lines,PKD1/3−/−
R1461 T1698 T1694 arg1Of lines,knockout
R1462 T1698 T1695 arg1Of lines,DT40
R1463 T1698 T1696 arg1Of lines,B
R1464 T1698 T1697 arg1Of lines,cell
R1465 T1701 T1699 arg2Of described,have
R1466 T1701 T1700 arg2Of described,been
R1467 T1701 T1702 arg1Of described,previously
R1468 T1701 T1703 arg1Of described,[
R1469 T1704 T1703 arg2Of 1,[
R1470 T1705 T1703 arg3Of ],[
R1471 T1706 T1707 arg1Of Cells,were
R1472 T1706 T1708 arg2Of Cells,lysed
R1473 T1708 T1707 arg2Of lysed,were
R1474 T1708 T1709 arg1Of lysed,and
R1475 T1711 T1710 arg1Of extracts,protein
R1476 T1711 T1712 arg1Of extracts,were
R1477 T1711 T1713 arg2Of extracts,analysed
R1478 T1713 T1709 arg2Of analysed,and
R1479 T1713 T1712 arg2Of analysed,were
R1480 T1713 T1714 arg1Of analysed,in
R1481 T1713 T1718 arg1Of analysed,as
R1482 T1717 T1714 arg2Of experiments,in
R1483 T1717 T1715 arg1Of experiments,Western
R1484 T1717 T1716 arg1Of experiments,blotting
R1485 T1720 T1719 arg1Of described,previously
R1486 T1722 T1718 arg2Of 1,as
R1487 T1722 T1720 arg1Of 1,described
R1488 T1722 T1721 arg2Of 1,[
R1489 T1723 T1721 arg3Of ],[
R1490 T1727 T1724 arg1Of assays,Chloramphenicol
R1491 T1727 T1725 arg1Of assays,acetyl
R1492 T1727 T1726 arg1Of assays,transferase
R1493 T1727 T1728 arg1Of assays,have
R1494 T1727 T1729 arg1Of assays,been
R1495 T1727 T1730 arg2Of assays,described
R1496 T1730 T1728 arg2Of described,have
R1497 T1730 T1729 arg2Of described,been
R1498 T1730 T1731 arg1Of described,previously
R1499 T1730 T1732 arg1Of described,[
R1500 T1733 T1732 arg2Of 29,[
R1501 T1734 T1732 arg3Of ],[
R1597 T1872 T1879 arg1Of cells,were
R1598 T1872 T1870 arg1Of cells,DT40
R1599 T1872 T1871 arg1Of cells,B
R1600 T1872 T1873 arg1Of cells,(
R1601 T1872 T1880 arg2Of cells,resuspended
R1602 T1875 T1874 arg1Of cells,2 × 106
R1603 T1875 T1876 arg1Of cells,per
R1604 T1875 T1873 arg2Of cells,(
R1605 T1877 T1876 arg2Of point,per
R1606 T1878 T1873 arg3Of ),(
R1607 T1880 T1879 arg2Of resuspended,were
R1608 T1880 T1881 arg1Of resuspended,in
R1609 T1883 T1881 arg2Of buffer,in
R1610 T1883 T1882 arg1Of buffer,200 μl
R1611 T1883 T1884 arg1Of buffer,(
R1612 T1883 T1895 arg1Of buffer,containing
R1613 T1887 T1884 arg2Of media,(
R1614 T1887 T1885 arg1Of media,RPMI
R1615 T1887 T1886 arg1Of media,1640
R1616 T1887 T1888 arg1Of media,","
R1617 T1889 T1890 arg1Of 1,%
R1618 T1893 T1888 arg2Of serum,","
R1619 T1893 T1889 arg1Of serum,1
R1620 T1893 T1891 arg1Of serum,foetal
R1621 T1893 T1892 arg1Of serum,calf
R1622 T1894 T1884 arg3Of ),(
R1623 T1899 T1895 arg2Of antibody,containing
R1624 T1899 T1896 arg1Of antibody,anti-chicken
R1625 T1899 T1897 arg1Of antibody,M1
R1626 T1899 T1898 arg1Of antibody,monoclonal
R1627 T1899 T1900 arg2Of antibody,conjugated
R1628 T1900 T1901 arg1Of conjugated,to
R1629 T1902 T1901 arg2Of FITC,to
R1630 T1902 T1903 arg1Of FITC,for
R1631 T1904 T1903 arg2Of 20 min,for
R1632 T1904 T1905 arg1Of 20 min,on
R1633 T1906 T1905 arg2Of ice,on
R1634 T1908 T1907 arg1Of cells,The
R1635 T1908 T1909 arg1Of cells,were
R1636 T1908 T1910 arg2Of cells,washed
R1637 T1910 T1909 arg2Of washed,were
R1638 T1910 T1911 arg1Of washed,twice
R1639 T1910 T1912 arg1Of washed,and
R1640 T1914 T1913 arg1Of intensity,fluorescent
R1641 T1914 T1915 arg1Of intensity,was
R1642 T1914 T1916 arg2Of intensity,analysed
R1643 T1916 T1912 arg2Of analysed,and
R1644 T1916 T1915 arg2Of analysed,was
R1645 T1919 T1916 arg1Of cytometry,analysed
R1646 T1919 T1917 arg2Of cytometry,by
R1647 T1919 T1918 arg1Of cytometry,flow
R1648 T1921 T1920 arg1Of results,All
R1649 T1921 T1922 arg2Of results,shown
R1650 T1921 T1923 arg1Of results,are
R1651 T1921 T1924 arg1Of results,representative
R1652 T1923 T1932 arg1Of are,unless
R1653 T1924 T1923 arg2Of representative,are
R1654 T1924 T1926 arg1Of representative,at
R1655 T1926 T1925 arg1Of at,of
R1656 T1927 T1928 arg1Of two,to
R1657 T1929 T1928 arg2Of four,to
R1658 T1931 T1926 arg2Of experiments,at
R1659 T1931 T1927 arg1Of experiments,two
R1660 T1931 T1930 arg1Of experiments,independent
R1661 T1934 T1932 arg2Of indicated,unless
R1662 T1934 T1933 arg1Of indicated,otherwise