Id |
Subject |
Object |
Predicate |
Lexical cue |
T1675 |
36-43 |
NN |
denotes |
culture |
T1676 |
43-44 |
-COMMA- |
denotes |
, |
T1677 |
45-54 |
JJ |
denotes |
transient |
T1678 |
55-68 |
NN |
denotes |
transfections |
T1679 |
69-72 |
CC |
denotes |
and |
T1680 |
73-77 |
NN |
denotes |
cell |
T1681 |
78-89 |
NN |
denotes |
stimulation |
T1682 |
90-93 |
DT |
denotes |
The |
T1683 |
94-104 |
NN |
denotes |
generation |
T1684 |
104-105 |
-COMMA- |
denotes |
, |
T1685 |
106-113 |
NN |
denotes |
culture |
T1686 |
114-117 |
CC |
denotes |
and |
T1687 |
118-128 |
NN |
denotes |
activation |
T1688 |
129-131 |
IN |
denotes |
of |
T1689 |
132-139 |
NN |
denotes |
PKD1−/− |
T1690 |
139-140 |
-COMMA- |
denotes |
, |
T1691 |
141-148 |
NN |
denotes |
PKD3−/− |
T1692 |
149-152 |
CC |
denotes |
and |
T1693 |
153-162 |
NN |
denotes |
PKD1/3−/− |
T1694 |
163-171 |
NN |
denotes |
knockout |
T1695 |
172-176 |
NN |
denotes |
DT40 |
T1696 |
177-178 |
NN |
denotes |
B |
T1697 |
179-183 |
NN |
denotes |
cell |
T1698 |
184-189 |
NN |
denotes |
lines |
T1699 |
190-194 |
VB |
denotes |
have |
T1700 |
195-199 |
VB |
denotes |
been |
T1701 |
200-209 |
VB |
denotes |
described |
T1702 |
210-220 |
RB |
denotes |
previously |
T1703 |
221-222 |
-LRB- |
denotes |
[ |
T1704 |
222-223 |
CD |
denotes |
1 |
T1705 |
223-224 |
-RRB- |
denotes |
] |
T1706 |
226-231 |
NN |
denotes |
Cells |
T1707 |
232-236 |
VB |
denotes |
were |
T1708 |
237-242 |
VB |
denotes |
lysed |
T1709 |
243-246 |
CC |
denotes |
and |
T1710 |
247-254 |
NN |
denotes |
protein |
T1711 |
255-263 |
NN |
denotes |
extracts |
T1712 |
264-268 |
VB |
denotes |
were |
T1713 |
269-277 |
VB |
denotes |
analysed |
T1714 |
278-280 |
IN |
denotes |
in |
T1715 |
281-288 |
JJ |
denotes |
Western |
T1716 |
289-297 |
NN |
denotes |
blotting |
T1717 |
298-309 |
NN |
denotes |
experiments |
T1718 |
310-312 |
IN |
denotes |
as |
T1719 |
313-323 |
RB |
denotes |
previously |
T1720 |
324-333 |
VB |
denotes |
described |
T1721 |
334-335 |
-LRB- |
denotes |
[ |
T1722 |
335-336 |
CD |
denotes |
1 |
T1723 |
336-337 |
-RRB- |
denotes |
] |
T1724 |
339-354 |
NN |
denotes |
Chloramphenicol |
T1725 |
355-361 |
NN |
denotes |
acetyl |
T1726 |
362-373 |
NN |
denotes |
transferase |
T1727 |
374-380 |
NN |
denotes |
assays |
T1728 |
381-385 |
VB |
denotes |
have |
T1729 |
386-390 |
VB |
denotes |
been |
T1730 |
391-400 |
VB |
denotes |
described |
T1731 |
401-411 |
RB |
denotes |
previously |
T1732 |
412-413 |
-LRB- |
denotes |
[ |
T1733 |
413-415 |
CD |
denotes |
29 |
T1734 |
415-416 |
-RRB- |
denotes |
] |
T1869 |
429-437 |
NN |
denotes |
staining |
T1870 |
438-442 |
NN |
denotes |
DT40 |
T1871 |
443-444 |
NN |
denotes |
B |
T1872 |
445-450 |
NN |
denotes |
cells |
T1873 |
451-452 |
-LRB- |
denotes |
( |
T1874 |
452-459 |
CD |
denotes |
2 × 106 |
T1875 |
460-465 |
NN |
denotes |
cells |
T1876 |
466-469 |
IN |
denotes |
per |
T1877 |
470-475 |
NN |
denotes |
point |
T1878 |
475-476 |
-RRB- |
denotes |
) |
T1879 |
477-481 |
VB |
denotes |
were |
T1880 |
482-493 |
VB |
denotes |
resuspended |
T1881 |
494-496 |
IN |
denotes |
in |
T1882 |
497-503 |
NN |
denotes |
200 μl |
T1883 |
504-510 |
NN |
denotes |
buffer |
T1884 |
511-512 |
-LRB- |
denotes |
( |
T1885 |
512-516 |
NN |
denotes |
RPMI |
T1886 |
517-521 |
CD |
denotes |
1640 |
T1887 |
522-527 |
NN |
denotes |
media |
T1888 |
527-528 |
-COMMA- |
denotes |
, |
T1889 |
529-530 |
CD |
denotes |
1 |
T1890 |
530-531 |
NN |
denotes |
% |
T1891 |
532-538 |
JJ |
denotes |
foetal |
T1892 |
539-543 |
NN |
denotes |
calf |
T1893 |
544-549 |
NN |
denotes |
serum |
T1894 |
549-550 |
-RRB- |
denotes |
) |
T1895 |
551-561 |
VB |
denotes |
containing |
T1896 |
562-574 |
JJ |
denotes |
anti-chicken |
T1897 |
575-577 |
NN |
denotes |
M1 |
T1898 |
578-588 |
JJ |
denotes |
monoclonal |
T1899 |
589-597 |
NN |
denotes |
antibody |
T1900 |
598-608 |
VB |
denotes |
conjugated |
T1901 |
609-611 |
TO |
denotes |
to |
T1902 |
612-616 |
NN |
denotes |
FITC |
T1903 |
617-620 |
IN |
denotes |
for |
T1904 |
621-627 |
NN |
denotes |
20 min |
T1905 |
628-630 |
IN |
denotes |
on |
T1906 |
631-634 |
NN |
denotes |
ice |
T1907 |
636-639 |
DT |
denotes |
The |
T1908 |
640-645 |
NN |
denotes |
cells |
T1909 |
646-650 |
VB |
denotes |
were |
T1910 |
651-657 |
VB |
denotes |
washed |
T1911 |
658-663 |
RB |
denotes |
twice |
T1912 |
664-667 |
CC |
denotes |
and |
T1913 |
668-679 |
JJ |
denotes |
fluorescent |
T1914 |
680-689 |
NN |
denotes |
intensity |
T1915 |
690-693 |
VB |
denotes |
was |
T1916 |
694-702 |
VB |
denotes |
analysed |
T1917 |
703-705 |
IN |
denotes |
by |
T1918 |
706-710 |
NN |
denotes |
flow |
T1919 |
711-720 |
NN |
denotes |
cytometry |
T1920 |
722-725 |
DT |
denotes |
All |
T1921 |
726-733 |
NN |
denotes |
results |
T1922 |
734-739 |
VB |
denotes |
shown |
T1923 |
740-743 |
VB |
denotes |
are |
T1924 |
744-758 |
JJ |
denotes |
representative |
T1925 |
759-761 |
IN |
denotes |
of |
T1926 |
762-764 |
IN |
denotes |
at |
T1927 |
765-768 |
CD |
denotes |
two |
T1928 |
769-771 |
TO |
denotes |
to |
T1929 |
772-776 |
CD |
denotes |
four |
T1930 |
777-788 |
JJ |
denotes |
independent |
T1931 |
789-800 |
NN |
denotes |
experiments |
T1932 |
801-807 |
IN |
denotes |
unless |
T1933 |
808-817 |
RB |
denotes |
otherwise |
T1934 |
818-827 |
VB |
denotes |
indicated |
R1438 |
T1675 |
T1676 |
arg1Of |
culture,"," |
R1439 |
T1676 |
T1679 |
arg1Of |
",",and |
R1440 |
T1678 |
T1676 |
arg2Of |
transfections,"," |
R1441 |
T1678 |
T1677 |
arg1Of |
transfections,transient |
R1442 |
T1681 |
T1679 |
arg2Of |
stimulation,and |
R1443 |
T1681 |
T1680 |
arg1Of |
stimulation,cell |
R1444 |
T1683 |
T1684 |
arg1Of |
generation,"," |
R1445 |
T1684 |
T1686 |
arg1Of |
",",and |
R1446 |
T1685 |
T1684 |
arg2Of |
culture,"," |
R1447 |
T1686 |
T1682 |
arg1Of |
and,The |
R1448 |
T1686 |
T1688 |
arg1Of |
and,of |
R1449 |
T1686 |
T1699 |
arg1Of |
and,have |
R1450 |
T1686 |
T1700 |
arg1Of |
and,been |
R1451 |
T1686 |
T1701 |
arg2Of |
and,described |
R1452 |
T1687 |
T1686 |
arg2Of |
activation,and |
R1453 |
T1689 |
T1690 |
arg1Of |
PKD1−/−,"," |
R1454 |
T1690 |
T1692 |
arg1Of |
",",and |
R1455 |
T1691 |
T1690 |
arg2Of |
PKD3−/−,"," |
R1456 |
T1693 |
T1692 |
arg2Of |
PKD1/3−/−,and |
R1457 |
T1698 |
T1688 |
arg2Of |
lines,of |
R1458 |
T1698 |
T1689 |
arg1Of |
lines,PKD1−/− |
R1459 |
T1698 |
T1691 |
arg1Of |
lines,PKD3−/− |
R1460 |
T1698 |
T1693 |
arg1Of |
lines,PKD1/3−/− |
R1461 |
T1698 |
T1694 |
arg1Of |
lines,knockout |
R1462 |
T1698 |
T1695 |
arg1Of |
lines,DT40 |
R1463 |
T1698 |
T1696 |
arg1Of |
lines,B |
R1464 |
T1698 |
T1697 |
arg1Of |
lines,cell |
R1465 |
T1701 |
T1699 |
arg2Of |
described,have |
R1466 |
T1701 |
T1700 |
arg2Of |
described,been |
R1467 |
T1701 |
T1702 |
arg1Of |
described,previously |
R1468 |
T1701 |
T1703 |
arg1Of |
described,[ |
R1469 |
T1704 |
T1703 |
arg2Of |
1,[ |
R1470 |
T1705 |
T1703 |
arg3Of |
],[ |
R1471 |
T1706 |
T1707 |
arg1Of |
Cells,were |
R1472 |
T1706 |
T1708 |
arg2Of |
Cells,lysed |
R1473 |
T1708 |
T1707 |
arg2Of |
lysed,were |
R1474 |
T1708 |
T1709 |
arg1Of |
lysed,and |
R1475 |
T1711 |
T1710 |
arg1Of |
extracts,protein |
R1476 |
T1711 |
T1712 |
arg1Of |
extracts,were |
R1477 |
T1711 |
T1713 |
arg2Of |
extracts,analysed |
R1478 |
T1713 |
T1709 |
arg2Of |
analysed,and |
R1479 |
T1713 |
T1712 |
arg2Of |
analysed,were |
R1480 |
T1713 |
T1714 |
arg1Of |
analysed,in |
R1481 |
T1713 |
T1718 |
arg1Of |
analysed,as |
R1482 |
T1717 |
T1714 |
arg2Of |
experiments,in |
R1483 |
T1717 |
T1715 |
arg1Of |
experiments,Western |
R1484 |
T1717 |
T1716 |
arg1Of |
experiments,blotting |
R1485 |
T1720 |
T1719 |
arg1Of |
described,previously |
R1486 |
T1722 |
T1718 |
arg2Of |
1,as |
R1487 |
T1722 |
T1720 |
arg1Of |
1,described |
R1488 |
T1722 |
T1721 |
arg2Of |
1,[ |
R1489 |
T1723 |
T1721 |
arg3Of |
],[ |
R1490 |
T1727 |
T1724 |
arg1Of |
assays,Chloramphenicol |
R1491 |
T1727 |
T1725 |
arg1Of |
assays,acetyl |
R1492 |
T1727 |
T1726 |
arg1Of |
assays,transferase |
R1493 |
T1727 |
T1728 |
arg1Of |
assays,have |
R1494 |
T1727 |
T1729 |
arg1Of |
assays,been |
R1495 |
T1727 |
T1730 |
arg2Of |
assays,described |
R1496 |
T1730 |
T1728 |
arg2Of |
described,have |
R1497 |
T1730 |
T1729 |
arg2Of |
described,been |
R1498 |
T1730 |
T1731 |
arg1Of |
described,previously |
R1499 |
T1730 |
T1732 |
arg1Of |
described,[ |
R1500 |
T1733 |
T1732 |
arg2Of |
29,[ |
R1501 |
T1734 |
T1732 |
arg3Of |
],[ |
R1597 |
T1872 |
T1879 |
arg1Of |
cells,were |
R1598 |
T1872 |
T1870 |
arg1Of |
cells,DT40 |
R1599 |
T1872 |
T1871 |
arg1Of |
cells,B |
R1600 |
T1872 |
T1873 |
arg1Of |
cells,( |
R1601 |
T1872 |
T1880 |
arg2Of |
cells,resuspended |
R1602 |
T1875 |
T1874 |
arg1Of |
cells,2 × 106 |
R1603 |
T1875 |
T1876 |
arg1Of |
cells,per |
R1604 |
T1875 |
T1873 |
arg2Of |
cells,( |
R1605 |
T1877 |
T1876 |
arg2Of |
point,per |
R1606 |
T1878 |
T1873 |
arg3Of |
),( |
R1607 |
T1880 |
T1879 |
arg2Of |
resuspended,were |
R1608 |
T1880 |
T1881 |
arg1Of |
resuspended,in |
R1609 |
T1883 |
T1881 |
arg2Of |
buffer,in |
R1610 |
T1883 |
T1882 |
arg1Of |
buffer,200 μl |
R1611 |
T1883 |
T1884 |
arg1Of |
buffer,( |
R1612 |
T1883 |
T1895 |
arg1Of |
buffer,containing |
R1613 |
T1887 |
T1884 |
arg2Of |
media,( |
R1614 |
T1887 |
T1885 |
arg1Of |
media,RPMI |
R1615 |
T1887 |
T1886 |
arg1Of |
media,1640 |
R1616 |
T1887 |
T1888 |
arg1Of |
media,"," |
R1617 |
T1889 |
T1890 |
arg1Of |
1,% |
R1618 |
T1893 |
T1888 |
arg2Of |
serum,"," |
R1619 |
T1893 |
T1889 |
arg1Of |
serum,1 |
R1620 |
T1893 |
T1891 |
arg1Of |
serum,foetal |
R1621 |
T1893 |
T1892 |
arg1Of |
serum,calf |
R1622 |
T1894 |
T1884 |
arg3Of |
),( |
R1623 |
T1899 |
T1895 |
arg2Of |
antibody,containing |
R1624 |
T1899 |
T1896 |
arg1Of |
antibody,anti-chicken |
R1625 |
T1899 |
T1897 |
arg1Of |
antibody,M1 |
R1626 |
T1899 |
T1898 |
arg1Of |
antibody,monoclonal |
R1627 |
T1899 |
T1900 |
arg2Of |
antibody,conjugated |
R1628 |
T1900 |
T1901 |
arg1Of |
conjugated,to |
R1629 |
T1902 |
T1901 |
arg2Of |
FITC,to |
R1630 |
T1902 |
T1903 |
arg1Of |
FITC,for |
R1631 |
T1904 |
T1903 |
arg2Of |
20 min,for |
R1632 |
T1904 |
T1905 |
arg1Of |
20 min,on |
R1633 |
T1906 |
T1905 |
arg2Of |
ice,on |
R1634 |
T1908 |
T1907 |
arg1Of |
cells,The |
R1635 |
T1908 |
T1909 |
arg1Of |
cells,were |
R1636 |
T1908 |
T1910 |
arg2Of |
cells,washed |
R1637 |
T1910 |
T1909 |
arg2Of |
washed,were |
R1638 |
T1910 |
T1911 |
arg1Of |
washed,twice |
R1639 |
T1910 |
T1912 |
arg1Of |
washed,and |
R1640 |
T1914 |
T1913 |
arg1Of |
intensity,fluorescent |
R1641 |
T1914 |
T1915 |
arg1Of |
intensity,was |
R1642 |
T1914 |
T1916 |
arg2Of |
intensity,analysed |
R1643 |
T1916 |
T1912 |
arg2Of |
analysed,and |
R1644 |
T1916 |
T1915 |
arg2Of |
analysed,was |
R1645 |
T1919 |
T1916 |
arg1Of |
cytometry,analysed |
R1646 |
T1919 |
T1917 |
arg2Of |
cytometry,by |
R1647 |
T1919 |
T1918 |
arg1Of |
cytometry,flow |
R1648 |
T1921 |
T1920 |
arg1Of |
results,All |
R1649 |
T1921 |
T1922 |
arg2Of |
results,shown |
R1650 |
T1921 |
T1923 |
arg1Of |
results,are |
R1651 |
T1921 |
T1924 |
arg1Of |
results,representative |
R1652 |
T1923 |
T1932 |
arg1Of |
are,unless |
R1653 |
T1924 |
T1923 |
arg2Of |
representative,are |
R1654 |
T1924 |
T1926 |
arg1Of |
representative,at |
R1655 |
T1926 |
T1925 |
arg1Of |
at,of |
R1656 |
T1927 |
T1928 |
arg1Of |
two,to |
R1657 |
T1929 |
T1928 |
arg2Of |
four,to |
R1658 |
T1931 |
T1926 |
arg2Of |
experiments,at |
R1659 |
T1931 |
T1927 |
arg1Of |
experiments,two |
R1660 |
T1931 |
T1930 |
arg1Of |
experiments,independent |
R1661 |
T1934 |
T1932 |
arg2Of |
indicated,unless |
R1662 |
T1934 |
T1933 |
arg1Of |
indicated,otherwise |