Id |
Subject |
Object |
Predicate |
Lexical cue |
T4371 |
0-20 |
JJ |
denotes |
Methylation-specific |
T4372 |
21-42 |
NN |
denotes |
restriction-PCR-assay |
T4373 |
43-46 |
NN |
denotes |
DNA |
T4374 |
47-50 |
VB |
denotes |
was |
T4375 |
51-60 |
VB |
denotes |
extracted |
T4376 |
61-65 |
IN |
denotes |
with |
T4377 |
66-67 |
DT |
denotes |
a |
T4378 |
68-78 |
JJ |
denotes |
commercial |
T4379 |
79-82 |
NN |
denotes |
kit |
T4380 |
83-84 |
-LRB- |
denotes |
( |
T4381 |
84-90 |
NN |
denotes |
Qiagen |
T4382 |
90-91 |
-RRB- |
denotes |
) |
T4383 |
92-94 |
IN |
denotes |
as |
T4384 |
95-106 |
VB |
denotes |
recommended |
T4385 |
108-113 |
IN |
denotes |
Since |
T4386 |
114-117 |
DT |
denotes |
the |
T4387 |
118-129 |
NN |
denotes |
restriction |
T4388 |
130-137 |
NN |
denotes |
ability |
T4389 |
138-140 |
IN |
denotes |
of |
T4390 |
141-148 |
JJ |
denotes |
several |
T4391 |
149-162 |
NN |
denotes |
endonucleases |
T4392 |
163-165 |
VB |
denotes |
is |
T4393 |
166-175 |
VB |
denotes |
inhibited |
T4394 |
176-178 |
IN |
denotes |
by |
T4395 |
179-190 |
NN |
denotes |
methylation |
T4396 |
191-193 |
IN |
denotes |
of |
T4397 |
194-199 |
PRP-DOLLAR- |
denotes |
their |
T4398 |
200-206 |
NN |
denotes |
target |
T4399 |
207-215 |
NN |
denotes |
sequence |
T4400 |
215-216 |
-COMMA- |
denotes |
, |
T4401 |
217-219 |
PRP |
denotes |
we |
T4402 |
220-224 |
VB |
denotes |
used |
T4403 |
225-246 |
JJ |
denotes |
methylation-sensitive |
T4404 |
247-254 |
NN |
denotes |
enzymes |
T4405 |
255-260 |
NN |
denotes |
HpaII |
T4406 |
261-264 |
CC |
denotes |
and |
T4407 |
265-282 |
NN |
denotes |
HaeII-isochizomer |
T4408 |
283-291 |
NN |
denotes |
Bsp143II |
T4409 |
292-295 |
CC |
denotes |
and |
T4410 |
296-304 |
NN |
denotes |
Bsh1236I |
T4411 |
305-306 |
-LRB- |
denotes |
( |
T4412 |
306-309 |
NNP |
denotes |
MBI |
T4413 |
310-319 |
NNP |
denotes |
Fermentas |
T4414 |
319-320 |
-COMMA- |
denotes |
, |
T4415 |
321-323 |
NNP |
denotes |
St |
T4416 |
324-332 |
NNP |
denotes |
Leon-Rot |
T4417 |
332-333 |
-COMMA- |
denotes |
, |
T4418 |
334-341 |
NNP |
denotes |
Germany |
T4419 |
341-342 |
-RRB- |
denotes |
) |
T4420 |
343-344 |
-LRB- |
denotes |
( |
T4421 |
344-349 |
CD |
denotes |
20,24 |
T4422 |
349-350 |
-RRB- |
denotes |
) |
T4423 |
352-354 |
IN |
denotes |
As |
T4424 |
355-362 |
VB |
denotes |
control |
T4425 |
363-366 |
DT |
denotes |
the |
T4426 |
367-388 |
JJ |
denotes |
methylation-resistant |
T4427 |
389-395 |
NN |
denotes |
enzyme |
T4428 |
396-400 |
NN |
denotes |
MspI |
T4429 |
401-404 |
CC |
denotes |
and |
T4430 |
405-407 |
DT |
denotes |
an |
T4431 |
408-414 |
NN |
denotes |
enzyme |
T4432 |
415-419 |
IN |
denotes |
with |
T4433 |
420-422 |
DT |
denotes |
no |
T4434 |
423-434 |
NN |
denotes |
recognition |
T4435 |
435-439 |
NN |
denotes |
site |
T4436 |
440-442 |
IN |
denotes |
in |
T4437 |
443-446 |
DT |
denotes |
the |
T4438 |
447-453 |
NN |
denotes |
target |
T4439 |
454-462 |
NN |
denotes |
promoter |
T4440 |
462-463 |
-COMMA- |
denotes |
, |
T4441 |
464-469 |
NN |
denotes |
EcoRI |
T4442 |
469-470 |
-COMMA- |
denotes |
, |
T4443 |
471-475 |
VB |
denotes |
were |
T4444 |
476-480 |
VB |
denotes |
used |
T4445 |
482-485 |
NN |
denotes |
DNA |
T4446 |
486-487 |
-LRB- |
denotes |
( |
T4447 |
487-490 |
CD |
denotes |
0.8 |
T4448 |
491-493 |
NN |
denotes |
µg |
T4449 |
493-494 |
-RRB- |
denotes |
) |
T4450 |
495-498 |
VB |
denotes |
was |
T4451 |
499-507 |
VB |
denotes |
digested |
T4452 |
508-510 |
IN |
denotes |
by |
T4453 |
511-513 |
CD |
denotes |
40 |
T4454 |
514-515 |
NN |
denotes |
U |
T4455 |
516-519 |
DT |
denotes |
the |
T4456 |
520-530 |
JJ |
denotes |
respective |
T4457 |
531-537 |
NN |
denotes |
enzyme |
T4458 |
538-541 |
IN |
denotes |
for |
T4459 |
542-543 |
CD |
denotes |
6 |
T4460 |
544-545 |
NN |
denotes |
h |
T4461 |
546-549 |
CC |
denotes |
and |
T4462 |
549-550 |
-COMMA- |
denotes |
, |
T4463 |
551-553 |
TO |
denotes |
to |
T4464 |
554-560 |
VB |
denotes |
ensure |
T4465 |
561-569 |
JJ |
denotes |
complete |
T4466 |
570-578 |
NN |
denotes |
cleavage |
T4467 |
578-579 |
-COMMA- |
denotes |
, |
T4468 |
580-590 |
JJ |
denotes |
additional |
T4469 |
591-593 |
CD |
denotes |
20 |
T4470 |
594-595 |
NN |
denotes |
U |
T4471 |
596-599 |
IN |
denotes |
for |
T4472 |
600-602 |
CD |
denotes |
16 |
T4473 |
603-604 |
NN |
denotes |
h |
T4474 |
606-616 |
RB |
denotes |
Thereafter |
T4475 |
617-620 |
CD |
denotes |
100 |
T4476 |
621-623 |
NN |
denotes |
ng |
T4477 |
624-626 |
IN |
denotes |
of |
T4478 |
627-635 |
JJ |
denotes |
digested |
T4479 |
636-639 |
NN |
denotes |
DNA |
T4480 |
640-643 |
VB |
denotes |
was |
T4481 |
644-648 |
VB |
denotes |
used |
T4482 |
649-651 |
TO |
denotes |
to |
T4483 |
652-653 |
DT |
denotes |
a |
T4484 |
654-657 |
NN |
denotes |
PCR |
T4485 |
658-671 |
NN |
denotes |
amplification |
T4486 |
672-674 |
IN |
denotes |
of |
T4487 |
675-678 |
CD |
denotes |
two |
T4488 |
679-688 |
NN |
denotes |
fragments |
T4489 |
689-690 |
-LRB- |
denotes |
( |
T4490 |
690-692 |
NN |
denotes |
F1 |
T4491 |
693-696 |
CC |
denotes |
and |
T4492 |
697-699 |
NN |
denotes |
F2 |
T4493 |
699-700 |
-RRB- |
denotes |
) |
T4494 |
701-709 |
VB |
denotes |
spanning |
T4495 |
710-714 |
NN |
denotes |
part |
T4496 |
715-717 |
IN |
denotes |
of |
T4497 |
718-721 |
DT |
denotes |
the |
T4498 |
722-727 |
NN |
denotes |
IRF-4 |
T4499 |
728-736 |
NN |
denotes |
promoter |
T4500 |
737-738 |
-LRB- |
denotes |
( |
T4501 |
738-740 |
CD |
denotes |
30 |
T4502 |
740-741 |
-RRB- |
denotes |
) |
T4503 |
742-743 |
-LRB- |
denotes |
( |
T4504 |
743-750 |
NN |
denotes |
GenBank |
T4505 |
751-757 |
NN |
denotes |
U52683 |
T4506 |
757-758 |
-COLON- |
denotes |
; |
T4507 |
759-762 |
VB |
denotes |
see |
T4508 |
763-769 |
NN |
denotes |
Figure |
T4509 |
770-772 |
NN |
denotes |
3A |
T4510 |
772-773 |
-RRB- |
denotes |
) |
T4511 |
775-778 |
DT |
denotes |
The |
T4512 |
779-788 |
NN |
denotes |
sequences |
T4513 |
789-791 |
IN |
denotes |
of |
T4514 |
792-795 |
DT |
denotes |
the |
T4515 |
796-803 |
NN |
denotes |
primers |
T4516 |
804-808 |
VB |
denotes |
were |
T4517 |
809-819 |
JJ |
denotes |
F1-forward |
T4518 |
819-820 |
-COLON- |
denotes |
: |
T4519 |
821-849 |
JJ |
denotes |
5′-TTGAGATGGAGTCTTGCTCTGT-3′ |
T4520 |
849-850 |
-COMMA- |
denotes |
, |
T4521 |
851-861 |
JJ |
denotes |
F1-reverse |
T4522 |
861-862 |
-COLON- |
denotes |
: |
T4523 |
863-891 |
NN |
denotes |
ATCACTTCCAGACTTCAGTTCACCT-3′ |
T4524 |
892-893 |
-LRB- |
denotes |
( |
T4525 |
893-896 |
CD |
denotes |
341 |
T4526 |
897-899 |
NN |
denotes |
bp |
T4527 |
899-900 |
-RRB- |
denotes |
) |
T4528 |
900-901 |
-COLON- |
denotes |
; |
T4529 |
902-912 |
JJ |
denotes |
F2-forward |
T4530 |
912-913 |
-COLON- |
denotes |
: |
T4531 |
914-945 |
JJ |
denotes |
5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′ |
T4532 |
945-946 |
-COMMA- |
denotes |
, |
T4533 |
947-957 |
JJ |
denotes |
F2-reverse |
T4534 |
957-958 |
-COLON- |
denotes |
: |
T4535 |
959-988 |
NN |
denotes |
5′-CCAGGACCTCAGGAGGCCAGTCA-3′ |
T4536 |
989-990 |
-LRB- |
denotes |
( |
T4537 |
990-993 |
CD |
denotes |
474 |
T4538 |
994-996 |
NN |
denotes |
bp |
T4539 |
996-997 |
-RRB- |
denotes |
) |
T4540 |
999-1002 |
DT |
denotes |
The |
T4541 |
1003-1006 |
NN |
denotes |
PCR |
T4542 |
1007-1017 |
NN |
denotes |
conditions |
T4543 |
1018-1022 |
VB |
denotes |
were |
T4544 |
1023-1032 |
VB |
denotes |
described |
T4545 |
1033-1042 |
RB |
denotes |
elsewhere |
T4546 |
1043-1044 |
-LRB- |
denotes |
( |
T4547 |
1044-1045 |
CD |
denotes |
3 |
T4548 |
1045-1046 |
-RRB- |
denotes |
) |
T4549 |
1048-1051 |
NN |
denotes |
PCR |
T4550 |
1052-1055 |
VB |
denotes |
was |
T4551 |
1056-1065 |
VB |
denotes |
performed |
T4552 |
1066-1070 |
IN |
denotes |
with |
T4553 |
1071-1073 |
DT |
denotes |
an |
T4554 |
1074-1083 |
JJ |
denotes |
annealing |
T4555 |
1084-1095 |
NN |
denotes |
temperature |
T4556 |
1096-1098 |
IN |
denotes |
of |
T4557 |
1099-1103 |
NN |
denotes |
62°C |
T4558 |
1104-1107 |
CC |
denotes |
and |
T4559 |
1108-1110 |
CD |
denotes |
35 |
T4560 |
1111-1117 |
NN |
denotes |
cycles |
T4561 |
1119-1123 |
WRB |
denotes |
When |
T4562 |
1124-1127 |
NN |
denotes |
DNA |
T4563 |
1128-1131 |
VB |
denotes |
was |
T4564 |
1132-1142 |
VB |
denotes |
methylated |
T4565 |
1143-1145 |
IN |
denotes |
at |
T4566 |
1146-1154 |
JJ |
denotes |
specific |
T4567 |
1155-1160 |
NN |
denotes |
sites |
T4568 |
1160-1161 |
-COMMA- |
denotes |
, |
T4569 |
1162-1165 |
DT |
denotes |
the |
T4570 |
1166-1175 |
JJ |
denotes |
sensitive |
T4571 |
1176-1183 |
NN |
denotes |
enzymes |
T4572 |
1184-1188 |
VB |
denotes |
were |
T4573 |
1189-1192 |
RB |
denotes |
not |
T4574 |
1193-1197 |
JJ |
denotes |
able |
T4575 |
1198-1200 |
TO |
denotes |
to |
T4576 |
1201-1207 |
VB |
denotes |
digest |
T4577 |
1208-1211 |
DT |
denotes |
the |
T4578 |
1212-1215 |
NN |
denotes |
DNA |
T4579 |
1216-1219 |
CC |
denotes |
and |
T4580 |
1220-1233 |
NN |
denotes |
amplification |
T4581 |
1234-1238 |
VB |
denotes |
took |
T4582 |
1239-1244 |
NN |
denotes |
place |
T4583 |
1244-1245 |
-COLON- |
denotes |
; |
T4584 |
1246-1248 |
IN |
denotes |
in |
T4585 |
1249-1253 |
NN |
denotes |
case |
T4586 |
1254-1256 |
IN |
denotes |
of |
T4587 |
1257-1259 |
DT |
denotes |
no |
T4588 |
1260-1271 |
NN |
denotes |
methylation |
T4589 |
1271-1272 |
-COMMA- |
denotes |
, |
T4590 |
1273-1276 |
NN |
denotes |
DNA |
T4591 |
1277-1280 |
VB |
denotes |
was |
T4592 |
1281-1289 |
VB |
denotes |
digested |
T4593 |
1290-1293 |
CC |
denotes |
and |
T4594 |
1294-1296 |
DT |
denotes |
no |
T4595 |
1297-1304 |
NN |
denotes |
product |
T4596 |
1305-1308 |
VB |
denotes |
was |
T4597 |
1309-1318 |
VB |
denotes |
generated |
T4598 |
1320-1323 |
DT |
denotes |
The |
T4599 |
1324-1327 |
NN |
denotes |
PCR |
T4600 |
1328-1336 |
NN |
denotes |
products |
T4601 |
1337-1341 |
VB |
denotes |
were |
T4602 |
1342-1357 |
VB |
denotes |
electrophoresed |
T4603 |
1358-1360 |
IN |
denotes |
on |
T4604 |
1361-1362 |
DT |
denotes |
a |
T4605 |
1363-1364 |
CD |
denotes |
3 |
T4606 |
1364-1365 |
NN |
denotes |
% |
T4607 |
1366-1373 |
NN |
denotes |
agarose |
T4608 |
1374-1377 |
NN |
denotes |
gel |
T4609 |
1377-1378 |
-COMMA- |
denotes |
, |
T4610 |
1379-1383 |
VB |
denotes |
were |
T4611 |
1384-1391 |
VB |
denotes |
stained |
T4612 |
1392-1396 |
IN |
denotes |
with |
T4613 |
1397-1405 |
NN |
denotes |
ethidium |
T4614 |
1406-1413 |
NN |
denotes |
bromide |
T4615 |
1414-1417 |
CC |
denotes |
and |
T4616 |
1418-1430 |
VB |
denotes |
photographed |
T4617 |
1432-1435 |
NN |
denotes |
PCR |
T4618 |
1436-1444 |
NN |
denotes |
products |
T4619 |
1445-1449 |
VB |
denotes |
were |
T4620 |
1450-1458 |
VB |
denotes |
verified |
T4621 |
1459-1461 |
IN |
denotes |
by |
T4622 |
1462-1471 |
VB |
denotes |
automated |
T4623 |
1472-1482 |
NN |
denotes |
sequencing |
R3523 |
T4372 |
T4371 |
arg1Of |
restriction-PCR-assay,Methylation-specific |
R3524 |
T4373 |
T4374 |
arg1Of |
DNA,was |
R3525 |
T4373 |
T4375 |
arg2Of |
DNA,extracted |
R3526 |
T4375 |
T4374 |
arg2Of |
extracted,was |
R3527 |
T4375 |
T4376 |
arg1Of |
extracted,with |
R3528 |
T4375 |
T4383 |
arg1Of |
extracted,as |
R3529 |
T4379 |
T4376 |
arg2Of |
kit,with |
R3530 |
T4379 |
T4377 |
arg1Of |
kit,a |
R3531 |
T4379 |
T4378 |
arg1Of |
kit,commercial |
R3532 |
T4379 |
T4380 |
arg1Of |
kit,( |
R3533 |
T4381 |
T4380 |
arg2Of |
Qiagen,( |
R3534 |
T4382 |
T4380 |
arg3Of |
),( |
R3535 |
T4384 |
T4383 |
arg2Of |
recommended,as |
R3536 |
T4388 |
T4386 |
arg1Of |
ability,the |
R3537 |
T4388 |
T4387 |
arg1Of |
ability,restriction |
R3538 |
T4388 |
T4389 |
arg1Of |
ability,of |
R3539 |
T4388 |
T4392 |
arg1Of |
ability,is |
R3540 |
T4388 |
T4393 |
arg2Of |
ability,inhibited |
R3541 |
T4391 |
T4389 |
arg2Of |
endonucleases,of |
R3542 |
T4391 |
T4390 |
arg1Of |
endonucleases,several |
R3543 |
T4393 |
T4385 |
arg2Of |
inhibited,Since |
R3544 |
T4393 |
T4392 |
arg2Of |
inhibited,is |
R3545 |
T4395 |
T4393 |
arg1Of |
methylation,inhibited |
R3546 |
T4395 |
T4394 |
arg2Of |
methylation,by |
R3547 |
T4395 |
T4396 |
arg1Of |
methylation,of |
R3548 |
T4399 |
T4396 |
arg2Of |
sequence,of |
R3549 |
T4399 |
T4397 |
arg1Of |
sequence,their |
R3550 |
T4399 |
T4398 |
arg1Of |
sequence,target |
R3551 |
T4401 |
T4402 |
arg1Of |
we,used |
R3552 |
T4402 |
T4385 |
arg1Of |
used,Since |
R3553 |
T4402 |
T4400 |
arg1Of |
used,"," |
R3554 |
T4405 |
T4403 |
arg1Of |
HpaII,methylation-sensitive |
R3555 |
T4405 |
T4404 |
arg1Of |
HpaII,enzymes |
R3556 |
T4405 |
T4406 |
arg1Of |
HpaII,and |
R3557 |
T4406 |
T4409 |
arg1Of |
and,and |
R3558 |
T4408 |
T4406 |
arg2Of |
Bsp143II,and |
R3559 |
T4408 |
T4407 |
arg1Of |
Bsp143II,HaeII-isochizomer |
R3560 |
T4409 |
T4402 |
arg2Of |
and,used |
R3561 |
T4409 |
T4420 |
arg1Of |
and,( |
R3562 |
T4410 |
T4409 |
arg2Of |
Bsh1236I,and |
R3563 |
T4410 |
T4411 |
arg1Of |
Bsh1236I,( |
R3564 |
T4413 |
T4411 |
arg2Of |
Fermentas,( |
R3565 |
T4413 |
T4412 |
arg1Of |
Fermentas,MBI |
R3566 |
T4413 |
T4414 |
arg1Of |
Fermentas,"," |
R3567 |
T4416 |
T4414 |
arg2Of |
Leon-Rot,"," |
R3568 |
T4416 |
T4415 |
arg1Of |
Leon-Rot,St |
R3569 |
T4416 |
T4417 |
arg1Of |
Leon-Rot,"," |
R3570 |
T4418 |
T4417 |
arg2Of |
Germany,"," |
R3571 |
T4419 |
T4411 |
arg3Of |
),( |
R3572 |
T4421 |
T4420 |
arg2Of |
"20,24",( |
R3573 |
T4422 |
T4420 |
arg3Of |
),( |
R3574 |
T4424 |
T4423 |
arg2Of |
control,As |
R3575 |
T4424 |
T4432 |
arg1Of |
control,with |
R3576 |
T4428 |
T4425 |
arg1Of |
MspI,the |
R3577 |
T4428 |
T4426 |
arg1Of |
MspI,methylation-resistant |
R3578 |
T4428 |
T4427 |
arg1Of |
MspI,enzyme |
R3579 |
T4428 |
T4429 |
arg1Of |
MspI,and |
R3580 |
T4429 |
T4424 |
arg2Of |
and,control |
R3581 |
T4431 |
T4429 |
arg2Of |
enzyme,and |
R3582 |
T4431 |
T4430 |
arg1Of |
enzyme,an |
R3583 |
T4435 |
T4432 |
arg2Of |
site,with |
R3584 |
T4435 |
T4433 |
arg1Of |
site,no |
R3585 |
T4435 |
T4434 |
arg1Of |
site,recognition |
R3586 |
T4435 |
T4436 |
arg1Of |
site,in |
R3587 |
T4439 |
T4436 |
arg2Of |
promoter,in |
R3588 |
T4439 |
T4437 |
arg1Of |
promoter,the |
R3589 |
T4439 |
T4438 |
arg1Of |
promoter,target |
R3590 |
T4441 |
T4443 |
arg1Of |
EcoRI,were |
R3591 |
T4441 |
T4444 |
arg2Of |
EcoRI,used |
R3592 |
T4444 |
T4423 |
arg1Of |
used,As |
R3593 |
T4444 |
T4440 |
arg1Of |
used,"," |
R3594 |
T4444 |
T4442 |
arg1Of |
used,"," |
R3595 |
T4444 |
T4443 |
arg2Of |
used,were |
R3596 |
T4445 |
T4446 |
arg1Of |
DNA,( |
R3597 |
T4445 |
T4450 |
arg1Of |
DNA,was |
R3598 |
T4445 |
T4451 |
arg2Of |
DNA,digested |
R3599 |
T4448 |
T4446 |
arg2Of |
µg,( |
R3600 |
T4448 |
T4447 |
arg1Of |
µg,0.8 |
R3601 |
T4449 |
T4446 |
arg3Of |
),( |
R3602 |
T4451 |
T4450 |
arg2Of |
digested,was |
R3603 |
T4451 |
T4462 |
arg1Of |
digested,"," |
R3604 |
T4451 |
T4463 |
modOf |
digested,to |
R3605 |
T4451 |
T4467 |
arg1Of |
digested,"," |
R3606 |
T4451 |
T4471 |
arg1Of |
digested,for |
R3607 |
T4454 |
T4453 |
arg1Of |
U,40 |
R3608 |
T4457 |
T4451 |
arg1Of |
enzyme,digested |
R3609 |
T4457 |
T4452 |
arg2Of |
enzyme,by |
R3610 |
T4457 |
T4454 |
arg1Of |
enzyme,U |
R3611 |
T4457 |
T4455 |
arg1Of |
enzyme,the |
R3612 |
T4457 |
T4456 |
arg1Of |
enzyme,respective |
R3613 |
T4457 |
T4458 |
arg1Of |
enzyme,for |
R3614 |
T4457 |
T4461 |
arg1Of |
enzyme,and |
R3615 |
T4460 |
T4458 |
arg2Of |
h,for |
R3616 |
T4460 |
T4459 |
arg1Of |
h,6 |
R3617 |
T4464 |
T4463 |
arg1Of |
ensure,to |
R3618 |
T4466 |
T4464 |
arg2Of |
cleavage,ensure |
R3619 |
T4466 |
T4465 |
arg1Of |
cleavage,complete |
R3620 |
T4470 |
T4468 |
arg1Of |
U,additional |
R3621 |
T4470 |
T4469 |
arg1Of |
U,20 |
R3622 |
T4471 |
T4470 |
arg1Of |
for,U |
R3623 |
T4473 |
T4471 |
arg2Of |
h,for |
R3624 |
T4473 |
T4472 |
arg1Of |
h,16 |
R3625 |
T4475 |
T4474 |
arg1Of |
100,Thereafter |
R3626 |
T4476 |
T4475 |
arg1Of |
ng,100 |
R3627 |
T4476 |
T4477 |
arg1Of |
ng,of |
R3628 |
T4476 |
T4480 |
arg1Of |
ng,was |
R3629 |
T4476 |
T4481 |
arg2Of |
ng,used |
R3630 |
T4479 |
T4477 |
arg2Of |
DNA,of |
R3631 |
T4479 |
T4478 |
arg1Of |
DNA,digested |
R3632 |
T4481 |
T4480 |
arg2Of |
used,was |
R3633 |
T4481 |
T4482 |
arg1Of |
used,to |
R3634 |
T4481 |
T4510 |
arg1Of |
used,) |
R3635 |
T4485 |
T4482 |
arg2Of |
amplification,to |
R3636 |
T4485 |
T4483 |
arg1Of |
amplification,a |
R3637 |
T4485 |
T4484 |
arg1Of |
amplification,PCR |
R3638 |
T4485 |
T4486 |
arg1Of |
amplification,of |
R3639 |
T4485 |
T4489 |
arg1Of |
amplification,( |
R3640 |
T4485 |
T4494 |
arg1Of |
amplification,spanning |
R3641 |
T4485 |
T4507 |
modOf |
amplification,see |
R3642 |
T4488 |
T4486 |
arg2Of |
fragments,of |
R3643 |
T4488 |
T4487 |
arg1Of |
fragments,two |
R3644 |
T4490 |
T4491 |
arg1Of |
F1,and |
R3645 |
T4491 |
T4489 |
arg2Of |
and,( |
R3646 |
T4492 |
T4491 |
arg2Of |
F2,and |
R3647 |
T4493 |
T4489 |
arg3Of |
),( |
R3648 |
T4494 |
T4506 |
arg1Of |
spanning,; |
R3649 |
T4495 |
T4494 |
arg2Of |
part,spanning |
R3650 |
T4495 |
T4496 |
arg1Of |
part,of |
R3651 |
T4501 |
T4500 |
arg2Of |
30,( |
R3652 |
T4502 |
T4500 |
arg3Of |
),( |
R3653 |
T4505 |
T4496 |
arg2Of |
U52683,of |
R3654 |
T4505 |
T4497 |
arg1Of |
U52683,the |
R3655 |
T4505 |
T4498 |
arg1Of |
U52683,IRF-4 |
R3656 |
T4505 |
T4499 |
arg1Of |
U52683,promoter |
R3657 |
T4505 |
T4500 |
arg1Of |
U52683,( |
R3658 |
T4505 |
T4503 |
arg1Of |
U52683,( |
R3659 |
T4505 |
T4504 |
arg1Of |
U52683,GenBank |
R3660 |
T4509 |
T4507 |
arg2Of |
3A,see |
R3661 |
T4509 |
T4508 |
arg1Of |
3A,Figure |
R3662 |
T4512 |
T4511 |
arg1Of |
sequences,The |
R3663 |
T4512 |
T4513 |
arg1Of |
sequences,of |
R3664 |
T4512 |
T4516 |
arg1Of |
sequences,were |
R3665 |
T4512 |
T4517 |
arg1Of |
sequences,F1-forward |
R3666 |
T4515 |
T4513 |
arg2Of |
primers,of |
R3667 |
T4515 |
T4514 |
arg1Of |
primers,the |
R3668 |
T4516 |
T4518 |
arg1Of |
were,: |
R3669 |
T4517 |
T4516 |
arg2Of |
F1-forward,were |
R3670 |
T4519 |
T4520 |
arg1Of |
5′-TTGAGATGGAGTCTTGCTCTGT-3′,"," |
R3671 |
T4520 |
T4522 |
arg1Of |
",",: |
R3672 |
T4521 |
T4520 |
arg2Of |
F1-reverse,"," |
R3673 |
T4523 |
T4519 |
arg1Of |
ATCACTTCCAGACTTCAGTTCACCT-3′,5′-TTGAGATGGAGTCTTGCTCTGT-3′ |
R3674 |
T4523 |
T4521 |
arg1Of |
ATCACTTCCAGACTTCAGTTCACCT-3′,F1-reverse |
R3675 |
T4523 |
T4524 |
arg1Of |
ATCACTTCCAGACTTCAGTTCACCT-3′,( |
R3676 |
T4523 |
T4528 |
arg1Of |
ATCACTTCCAGACTTCAGTTCACCT-3′,; |
R3677 |
T4526 |
T4524 |
arg2Of |
bp,( |
R3678 |
T4526 |
T4525 |
arg1Of |
bp,341 |
R3679 |
T4527 |
T4524 |
arg3Of |
),( |
R3680 |
T4529 |
T4528 |
arg2Of |
F2-forward,; |
R3681 |
T4529 |
T4530 |
arg1Of |
F2-forward,: |
R3682 |
T4531 |
T4532 |
arg1Of |
5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′,"," |
R3683 |
T4532 |
T4534 |
arg1Of |
",",: |
R3684 |
T4533 |
T4532 |
arg2Of |
F2-reverse,"," |
R3685 |
T4535 |
T4536 |
arg1Of |
5′-CCAGGACCTCAGGAGGCCAGTCA-3′,( |
R3686 |
T4538 |
T4536 |
arg2Of |
bp,( |
R3687 |
T4538 |
T4537 |
arg1Of |
bp,474 |
R3688 |
T4539 |
T4536 |
arg3Of |
),( |
R3689 |
T4542 |
T4540 |
arg1Of |
conditions,The |
R3690 |
T4542 |
T4541 |
arg1Of |
conditions,PCR |
R3691 |
T4542 |
T4543 |
arg1Of |
conditions,were |
R3692 |
T4542 |
T4544 |
arg2Of |
conditions,described |
R3693 |
T4544 |
T4543 |
arg2Of |
described,were |
R3694 |
T4544 |
T4545 |
arg1Of |
described,elsewhere |
R3695 |
T4545 |
T4546 |
arg1Of |
elsewhere,( |
R3696 |
T4547 |
T4546 |
arg2Of |
3,( |
R3697 |
T4548 |
T4546 |
arg3Of |
),( |
R3698 |
T4549 |
T4550 |
arg1Of |
PCR,was |
R3699 |
T4549 |
T4551 |
arg2Of |
PCR,performed |
R3700 |
T4551 |
T4550 |
arg2Of |
performed,was |
R3701 |
T4551 |
T4552 |
arg1Of |
performed,with |
R3702 |
T4555 |
T4552 |
arg2Of |
temperature,with |
R3703 |
T4555 |
T4553 |
arg1Of |
temperature,an |
R3704 |
T4555 |
T4554 |
arg1Of |
temperature,annealing |
R3705 |
T4555 |
T4556 |
arg1Of |
temperature,of |
R3706 |
T4557 |
T4558 |
arg1Of |
62°C,and |
R3707 |
T4558 |
T4556 |
arg2Of |
and,of |
R3708 |
T4560 |
T4558 |
arg2Of |
cycles,and |
R3709 |
T4560 |
T4559 |
arg1Of |
cycles,35 |
R3710 |
T4562 |
T4563 |
arg1Of |
DNA,was |
R3711 |
T4562 |
T4564 |
arg2Of |
DNA,methylated |
R3712 |
T4564 |
T4561 |
arg2Of |
methylated,When |
R3713 |
T4564 |
T4563 |
arg2Of |
methylated,was |
R3714 |
T4564 |
T4565 |
arg1Of |
methylated,at |
R3715 |
T4567 |
T4565 |
arg2Of |
sites,at |
R3716 |
T4567 |
T4566 |
arg1Of |
sites,specific |
R3717 |
T4571 |
T4569 |
arg1Of |
enzymes,the |
R3718 |
T4571 |
T4570 |
arg1Of |
enzymes,sensitive |
R3719 |
T4571 |
T4572 |
arg1Of |
enzymes,were |
R3720 |
T4571 |
T4574 |
arg1Of |
enzymes,able |
R3721 |
T4571 |
T4576 |
arg1Of |
enzymes,digest |
R3722 |
T4572 |
T4573 |
arg1Of |
were,not |
R3723 |
T4572 |
T4579 |
arg1Of |
were,and |
R3724 |
T4574 |
T4572 |
arg2Of |
able,were |
R3725 |
T4576 |
T4574 |
arg2Of |
digest,able |
R3726 |
T4576 |
T4575 |
arg1Of |
digest,to |
R3727 |
T4578 |
T4576 |
arg2Of |
DNA,digest |
R3728 |
T4578 |
T4577 |
arg1Of |
DNA,the |
R3729 |
T4579 |
T4561 |
arg1Of |
and,When |
R3730 |
T4579 |
T4568 |
arg1Of |
and,"," |
R3731 |
T4579 |
T4583 |
arg1Of |
and,; |
R3732 |
T4580 |
T4581 |
arg1Of |
amplification,took |
R3733 |
T4581 |
T4579 |
arg2Of |
took,and |
R3734 |
T4582 |
T4581 |
arg2Of |
place,took |
R3735 |
T4585 |
T4584 |
arg2Of |
case,in |
R3736 |
T4585 |
T4586 |
arg1Of |
case,of |
R3737 |
T4588 |
T4586 |
arg2Of |
methylation,of |
R3738 |
T4588 |
T4587 |
arg1Of |
methylation,no |
R3739 |
T4590 |
T4591 |
arg1Of |
DNA,was |
R3740 |
T4590 |
T4592 |
arg2Of |
DNA,digested |
R3741 |
T4592 |
T4591 |
arg2Of |
digested,was |
R3742 |
T4592 |
T4593 |
arg1Of |
digested,and |
R3743 |
T4593 |
T4583 |
arg2Of |
and,; |
R3744 |
T4593 |
T4584 |
arg1Of |
and,in |
R3745 |
T4593 |
T4589 |
arg1Of |
and,"," |
R3746 |
T4595 |
T4594 |
arg1Of |
product,no |
R3747 |
T4595 |
T4596 |
arg1Of |
product,was |
R3748 |
T4595 |
T4597 |
arg2Of |
product,generated |
R3749 |
T4597 |
T4593 |
arg2Of |
generated,and |
R3750 |
T4597 |
T4596 |
arg2Of |
generated,was |
R3751 |
T4600 |
T4598 |
arg1Of |
products,The |
R3752 |
T4600 |
T4599 |
arg1Of |
products,PCR |
R3753 |
T4600 |
T4601 |
arg1Of |
products,were |
R3754 |
T4600 |
T4602 |
arg2Of |
products,electrophoresed |
R3755 |
T4600 |
T4610 |
arg1Of |
products,were |
R3756 |
T4600 |
T4611 |
arg2Of |
products,stained |
R3757 |
T4600 |
T4616 |
arg2Of |
products,photographed |
R3758 |
T4602 |
T4601 |
arg2Of |
electrophoresed,were |
R3759 |
T4602 |
T4603 |
arg1Of |
electrophoresed,on |
R3760 |
T4602 |
T4609 |
arg1Of |
electrophoresed,"," |
R3761 |
T4605 |
T4606 |
arg1Of |
3,% |
R3762 |
T4608 |
T4603 |
arg2Of |
gel,on |
R3763 |
T4608 |
T4604 |
arg1Of |
gel,a |
R3764 |
T4608 |
T4605 |
arg1Of |
gel,3 |
R3765 |
T4608 |
T4607 |
arg1Of |
gel,agarose |
R3766 |
T4611 |
T4612 |
arg1Of |
stained,with |
R3767 |
T4611 |
T4615 |
arg1Of |
stained,and |
R3768 |
T4614 |
T4612 |
arg2Of |
bromide,with |
R3769 |
T4614 |
T4613 |
arg1Of |
bromide,ethidium |
R3770 |
T4615 |
T4609 |
arg2Of |
and,"," |
R3771 |
T4615 |
T4610 |
arg2Of |
and,were |
R3772 |
T4616 |
T4615 |
arg2Of |
photographed,and |
R3773 |
T4618 |
T4617 |
arg1Of |
products,PCR |
R3774 |
T4618 |
T4619 |
arg1Of |
products,were |
R3775 |
T4618 |
T4620 |
arg2Of |
products,verified |
R3776 |
T4620 |
T4619 |
arg2Of |
verified,were |
R3777 |
T4623 |
T4620 |
arg1Of |
sequencing,verified |
R3778 |
T4623 |
T4621 |
arg2Of |
sequencing,by |
R3779 |
T4623 |
T4622 |
arg2Of |
sequencing,automated |