PMC:1310901 / 8718-10201 JSONTXT 14 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4371 0-20 JJ denotes Methylation-specific
T4372 21-42 NN denotes restriction-PCR-assay
T4373 43-46 NN denotes DNA
T4374 47-50 VB denotes was
T4375 51-60 VB denotes extracted
T4376 61-65 IN denotes with
T4377 66-67 DT denotes a
T4378 68-78 JJ denotes commercial
T4379 79-82 NN denotes kit
T4380 83-84 -LRB- denotes (
T4381 84-90 NN denotes Qiagen
T4382 90-91 -RRB- denotes )
T4383 92-94 IN denotes as
T4384 95-106 VB denotes recommended
T4385 108-113 IN denotes Since
T4386 114-117 DT denotes the
T4387 118-129 NN denotes restriction
T4388 130-137 NN denotes ability
T4389 138-140 IN denotes of
T4390 141-148 JJ denotes several
T4391 149-162 NN denotes endonucleases
T4392 163-165 VB denotes is
T4393 166-175 VB denotes inhibited
T4394 176-178 IN denotes by
T4395 179-190 NN denotes methylation
T4396 191-193 IN denotes of
T4397 194-199 PRP-DOLLAR- denotes their
T4398 200-206 NN denotes target
T4399 207-215 NN denotes sequence
T4400 215-216 -COMMA- denotes ,
T4401 217-219 PRP denotes we
T4402 220-224 VB denotes used
T4403 225-246 JJ denotes methylation-sensitive
T4404 247-254 NN denotes enzymes
T4405 255-260 NN denotes HpaII
T4406 261-264 CC denotes and
T4407 265-282 NN denotes HaeII-isochizomer
T4408 283-291 NN denotes Bsp143II
T4409 292-295 CC denotes and
T4410 296-304 NN denotes Bsh1236I
T4411 305-306 -LRB- denotes (
T4412 306-309 NNP denotes MBI
T4413 310-319 NNP denotes Fermentas
T4414 319-320 -COMMA- denotes ,
T4415 321-323 NNP denotes St
T4416 324-332 NNP denotes Leon-Rot
T4417 332-333 -COMMA- denotes ,
T4418 334-341 NNP denotes Germany
T4419 341-342 -RRB- denotes )
T4420 343-344 -LRB- denotes (
T4421 344-349 CD denotes 20,24
T4422 349-350 -RRB- denotes )
T4423 352-354 IN denotes As
T4424 355-362 VB denotes control
T4425 363-366 DT denotes the
T4426 367-388 JJ denotes methylation-resistant
T4427 389-395 NN denotes enzyme
T4428 396-400 NN denotes MspI
T4429 401-404 CC denotes and
T4430 405-407 DT denotes an
T4431 408-414 NN denotes enzyme
T4432 415-419 IN denotes with
T4433 420-422 DT denotes no
T4434 423-434 NN denotes recognition
T4435 435-439 NN denotes site
T4436 440-442 IN denotes in
T4437 443-446 DT denotes the
T4438 447-453 NN denotes target
T4439 454-462 NN denotes promoter
T4440 462-463 -COMMA- denotes ,
T4441 464-469 NN denotes EcoRI
T4442 469-470 -COMMA- denotes ,
T4443 471-475 VB denotes were
T4444 476-480 VB denotes used
T4445 482-485 NN denotes DNA
T4446 486-487 -LRB- denotes (
T4447 487-490 CD denotes 0.8
T4448 491-493 NN denotes µg
T4449 493-494 -RRB- denotes )
T4450 495-498 VB denotes was
T4451 499-507 VB denotes digested
T4452 508-510 IN denotes by
T4453 511-513 CD denotes 40
T4454 514-515 NN denotes U
T4455 516-519 DT denotes the
T4456 520-530 JJ denotes respective
T4457 531-537 NN denotes enzyme
T4458 538-541 IN denotes for
T4459 542-543 CD denotes 6
T4460 544-545 NN denotes h
T4461 546-549 CC denotes and
T4462 549-550 -COMMA- denotes ,
T4463 551-553 TO denotes to
T4464 554-560 VB denotes ensure
T4465 561-569 JJ denotes complete
T4466 570-578 NN denotes cleavage
T4467 578-579 -COMMA- denotes ,
T4468 580-590 JJ denotes additional
T4469 591-593 CD denotes 20
T4470 594-595 NN denotes U
T4471 596-599 IN denotes for
T4472 600-602 CD denotes 16
T4473 603-604 NN denotes h
T4474 606-616 RB denotes Thereafter
T4475 617-620 CD denotes 100
T4476 621-623 NN denotes ng
T4477 624-626 IN denotes of
T4478 627-635 JJ denotes digested
T4479 636-639 NN denotes DNA
T4480 640-643 VB denotes was
T4481 644-648 VB denotes used
T4482 649-651 TO denotes to
T4483 652-653 DT denotes a
T4484 654-657 NN denotes PCR
T4485 658-671 NN denotes amplification
T4486 672-674 IN denotes of
T4487 675-678 CD denotes two
T4488 679-688 NN denotes fragments
T4489 689-690 -LRB- denotes (
T4490 690-692 NN denotes F1
T4491 693-696 CC denotes and
T4492 697-699 NN denotes F2
T4493 699-700 -RRB- denotes )
T4494 701-709 VB denotes spanning
T4495 710-714 NN denotes part
T4496 715-717 IN denotes of
T4497 718-721 DT denotes the
T4498 722-727 NN denotes IRF-4
T4499 728-736 NN denotes promoter
T4500 737-738 -LRB- denotes (
T4501 738-740 CD denotes 30
T4502 740-741 -RRB- denotes )
T4503 742-743 -LRB- denotes (
T4504 743-750 NN denotes GenBank
T4505 751-757 NN denotes U52683
T4506 757-758 -COLON- denotes ;
T4507 759-762 VB denotes see
T4508 763-769 NN denotes Figure
T4509 770-772 NN denotes 3A
T4510 772-773 -RRB- denotes )
T4511 775-778 DT denotes The
T4512 779-788 NN denotes sequences
T4513 789-791 IN denotes of
T4514 792-795 DT denotes the
T4515 796-803 NN denotes primers
T4516 804-808 VB denotes were
T4517 809-819 JJ denotes F1-forward
T4518 819-820 -COLON- denotes :
T4519 821-849 JJ denotes 5′-TTGAGATGGAGTCTTGCTCTGT-3′
T4520 849-850 -COMMA- denotes ,
T4521 851-861 JJ denotes F1-reverse
T4522 861-862 -COLON- denotes :
T4523 863-891 NN denotes ATCACTTCCAGACTTCAGTTCACCT-3′
T4524 892-893 -LRB- denotes (
T4525 893-896 CD denotes 341
T4526 897-899 NN denotes bp
T4527 899-900 -RRB- denotes )
T4528 900-901 -COLON- denotes ;
T4529 902-912 JJ denotes F2-forward
T4530 912-913 -COLON- denotes :
T4531 914-945 JJ denotes 5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′
T4532 945-946 -COMMA- denotes ,
T4533 947-957 JJ denotes F2-reverse
T4534 957-958 -COLON- denotes :
T4535 959-988 NN denotes 5′-CCAGGACCTCAGGAGGCCAGTCA-3′
T4536 989-990 -LRB- denotes (
T4537 990-993 CD denotes 474
T4538 994-996 NN denotes bp
T4539 996-997 -RRB- denotes )
T4540 999-1002 DT denotes The
T4541 1003-1006 NN denotes PCR
T4542 1007-1017 NN denotes conditions
T4543 1018-1022 VB denotes were
T4544 1023-1032 VB denotes described
T4545 1033-1042 RB denotes elsewhere
T4546 1043-1044 -LRB- denotes (
T4547 1044-1045 CD denotes 3
T4548 1045-1046 -RRB- denotes )
T4549 1048-1051 NN denotes PCR
T4550 1052-1055 VB denotes was
T4551 1056-1065 VB denotes performed
T4552 1066-1070 IN denotes with
T4553 1071-1073 DT denotes an
T4554 1074-1083 JJ denotes annealing
T4555 1084-1095 NN denotes temperature
T4556 1096-1098 IN denotes of
T4557 1099-1103 NN denotes 62°C
T4558 1104-1107 CC denotes and
T4559 1108-1110 CD denotes 35
T4560 1111-1117 NN denotes cycles
T4561 1119-1123 WRB denotes When
T4562 1124-1127 NN denotes DNA
T4563 1128-1131 VB denotes was
T4564 1132-1142 VB denotes methylated
T4565 1143-1145 IN denotes at
T4566 1146-1154 JJ denotes specific
T4567 1155-1160 NN denotes sites
T4568 1160-1161 -COMMA- denotes ,
T4569 1162-1165 DT denotes the
T4570 1166-1175 JJ denotes sensitive
T4571 1176-1183 NN denotes enzymes
T4572 1184-1188 VB denotes were
T4573 1189-1192 RB denotes not
T4574 1193-1197 JJ denotes able
T4575 1198-1200 TO denotes to
T4576 1201-1207 VB denotes digest
T4577 1208-1211 DT denotes the
T4578 1212-1215 NN denotes DNA
T4579 1216-1219 CC denotes and
T4580 1220-1233 NN denotes amplification
T4581 1234-1238 VB denotes took
T4582 1239-1244 NN denotes place
T4583 1244-1245 -COLON- denotes ;
T4584 1246-1248 IN denotes in
T4585 1249-1253 NN denotes case
T4586 1254-1256 IN denotes of
T4587 1257-1259 DT denotes no
T4588 1260-1271 NN denotes methylation
T4589 1271-1272 -COMMA- denotes ,
T4590 1273-1276 NN denotes DNA
T4591 1277-1280 VB denotes was
T4592 1281-1289 VB denotes digested
T4593 1290-1293 CC denotes and
T4594 1294-1296 DT denotes no
T4595 1297-1304 NN denotes product
T4596 1305-1308 VB denotes was
T4597 1309-1318 VB denotes generated
T4598 1320-1323 DT denotes The
T4599 1324-1327 NN denotes PCR
T4600 1328-1336 NN denotes products
T4601 1337-1341 VB denotes were
T4602 1342-1357 VB denotes electrophoresed
T4603 1358-1360 IN denotes on
T4604 1361-1362 DT denotes a
T4605 1363-1364 CD denotes 3
T4606 1364-1365 NN denotes %
T4607 1366-1373 NN denotes agarose
T4608 1374-1377 NN denotes gel
T4609 1377-1378 -COMMA- denotes ,
T4610 1379-1383 VB denotes were
T4611 1384-1391 VB denotes stained
T4612 1392-1396 IN denotes with
T4613 1397-1405 NN denotes ethidium
T4614 1406-1413 NN denotes bromide
T4615 1414-1417 CC denotes and
T4616 1418-1430 VB denotes photographed
T4617 1432-1435 NN denotes PCR
T4618 1436-1444 NN denotes products
T4619 1445-1449 VB denotes were
T4620 1450-1458 VB denotes verified
T4621 1459-1461 IN denotes by
T4622 1462-1471 VB denotes automated
T4623 1472-1482 NN denotes sequencing
R3523 T4372 T4371 arg1Of restriction-PCR-assay,Methylation-specific
R3524 T4373 T4374 arg1Of DNA,was
R3525 T4373 T4375 arg2Of DNA,extracted
R3526 T4375 T4374 arg2Of extracted,was
R3527 T4375 T4376 arg1Of extracted,with
R3528 T4375 T4383 arg1Of extracted,as
R3529 T4379 T4376 arg2Of kit,with
R3530 T4379 T4377 arg1Of kit,a
R3531 T4379 T4378 arg1Of kit,commercial
R3532 T4379 T4380 arg1Of kit,(
R3533 T4381 T4380 arg2Of Qiagen,(
R3534 T4382 T4380 arg3Of ),(
R3535 T4384 T4383 arg2Of recommended,as
R3536 T4388 T4386 arg1Of ability,the
R3537 T4388 T4387 arg1Of ability,restriction
R3538 T4388 T4389 arg1Of ability,of
R3539 T4388 T4392 arg1Of ability,is
R3540 T4388 T4393 arg2Of ability,inhibited
R3541 T4391 T4389 arg2Of endonucleases,of
R3542 T4391 T4390 arg1Of endonucleases,several
R3543 T4393 T4385 arg2Of inhibited,Since
R3544 T4393 T4392 arg2Of inhibited,is
R3545 T4395 T4393 arg1Of methylation,inhibited
R3546 T4395 T4394 arg2Of methylation,by
R3547 T4395 T4396 arg1Of methylation,of
R3548 T4399 T4396 arg2Of sequence,of
R3549 T4399 T4397 arg1Of sequence,their
R3550 T4399 T4398 arg1Of sequence,target
R3551 T4401 T4402 arg1Of we,used
R3552 T4402 T4385 arg1Of used,Since
R3553 T4402 T4400 arg1Of used,","
R3554 T4405 T4403 arg1Of HpaII,methylation-sensitive
R3555 T4405 T4404 arg1Of HpaII,enzymes
R3556 T4405 T4406 arg1Of HpaII,and
R3557 T4406 T4409 arg1Of and,and
R3558 T4408 T4406 arg2Of Bsp143II,and
R3559 T4408 T4407 arg1Of Bsp143II,HaeII-isochizomer
R3560 T4409 T4402 arg2Of and,used
R3561 T4409 T4420 arg1Of and,(
R3562 T4410 T4409 arg2Of Bsh1236I,and
R3563 T4410 T4411 arg1Of Bsh1236I,(
R3564 T4413 T4411 arg2Of Fermentas,(
R3565 T4413 T4412 arg1Of Fermentas,MBI
R3566 T4413 T4414 arg1Of Fermentas,","
R3567 T4416 T4414 arg2Of Leon-Rot,","
R3568 T4416 T4415 arg1Of Leon-Rot,St
R3569 T4416 T4417 arg1Of Leon-Rot,","
R3570 T4418 T4417 arg2Of Germany,","
R3571 T4419 T4411 arg3Of ),(
R3572 T4421 T4420 arg2Of "20,24",(
R3573 T4422 T4420 arg3Of ),(
R3574 T4424 T4423 arg2Of control,As
R3575 T4424 T4432 arg1Of control,with
R3576 T4428 T4425 arg1Of MspI,the
R3577 T4428 T4426 arg1Of MspI,methylation-resistant
R3578 T4428 T4427 arg1Of MspI,enzyme
R3579 T4428 T4429 arg1Of MspI,and
R3580 T4429 T4424 arg2Of and,control
R3581 T4431 T4429 arg2Of enzyme,and
R3582 T4431 T4430 arg1Of enzyme,an
R3583 T4435 T4432 arg2Of site,with
R3584 T4435 T4433 arg1Of site,no
R3585 T4435 T4434 arg1Of site,recognition
R3586 T4435 T4436 arg1Of site,in
R3587 T4439 T4436 arg2Of promoter,in
R3588 T4439 T4437 arg1Of promoter,the
R3589 T4439 T4438 arg1Of promoter,target
R3590 T4441 T4443 arg1Of EcoRI,were
R3591 T4441 T4444 arg2Of EcoRI,used
R3592 T4444 T4423 arg1Of used,As
R3593 T4444 T4440 arg1Of used,","
R3594 T4444 T4442 arg1Of used,","
R3595 T4444 T4443 arg2Of used,were
R3596 T4445 T4446 arg1Of DNA,(
R3597 T4445 T4450 arg1Of DNA,was
R3598 T4445 T4451 arg2Of DNA,digested
R3599 T4448 T4446 arg2Of µg,(
R3600 T4448 T4447 arg1Of µg,0.8
R3601 T4449 T4446 arg3Of ),(
R3602 T4451 T4450 arg2Of digested,was
R3603 T4451 T4462 arg1Of digested,","
R3604 T4451 T4463 modOf digested,to
R3605 T4451 T4467 arg1Of digested,","
R3606 T4451 T4471 arg1Of digested,for
R3607 T4454 T4453 arg1Of U,40
R3608 T4457 T4451 arg1Of enzyme,digested
R3609 T4457 T4452 arg2Of enzyme,by
R3610 T4457 T4454 arg1Of enzyme,U
R3611 T4457 T4455 arg1Of enzyme,the
R3612 T4457 T4456 arg1Of enzyme,respective
R3613 T4457 T4458 arg1Of enzyme,for
R3614 T4457 T4461 arg1Of enzyme,and
R3615 T4460 T4458 arg2Of h,for
R3616 T4460 T4459 arg1Of h,6
R3617 T4464 T4463 arg1Of ensure,to
R3618 T4466 T4464 arg2Of cleavage,ensure
R3619 T4466 T4465 arg1Of cleavage,complete
R3620 T4470 T4468 arg1Of U,additional
R3621 T4470 T4469 arg1Of U,20
R3622 T4471 T4470 arg1Of for,U
R3623 T4473 T4471 arg2Of h,for
R3624 T4473 T4472 arg1Of h,16
R3625 T4475 T4474 arg1Of 100,Thereafter
R3626 T4476 T4475 arg1Of ng,100
R3627 T4476 T4477 arg1Of ng,of
R3628 T4476 T4480 arg1Of ng,was
R3629 T4476 T4481 arg2Of ng,used
R3630 T4479 T4477 arg2Of DNA,of
R3631 T4479 T4478 arg1Of DNA,digested
R3632 T4481 T4480 arg2Of used,was
R3633 T4481 T4482 arg1Of used,to
R3634 T4481 T4510 arg1Of used,)
R3635 T4485 T4482 arg2Of amplification,to
R3636 T4485 T4483 arg1Of amplification,a
R3637 T4485 T4484 arg1Of amplification,PCR
R3638 T4485 T4486 arg1Of amplification,of
R3639 T4485 T4489 arg1Of amplification,(
R3640 T4485 T4494 arg1Of amplification,spanning
R3641 T4485 T4507 modOf amplification,see
R3642 T4488 T4486 arg2Of fragments,of
R3643 T4488 T4487 arg1Of fragments,two
R3644 T4490 T4491 arg1Of F1,and
R3645 T4491 T4489 arg2Of and,(
R3646 T4492 T4491 arg2Of F2,and
R3647 T4493 T4489 arg3Of ),(
R3648 T4494 T4506 arg1Of spanning,;
R3649 T4495 T4494 arg2Of part,spanning
R3650 T4495 T4496 arg1Of part,of
R3651 T4501 T4500 arg2Of 30,(
R3652 T4502 T4500 arg3Of ),(
R3653 T4505 T4496 arg2Of U52683,of
R3654 T4505 T4497 arg1Of U52683,the
R3655 T4505 T4498 arg1Of U52683,IRF-4
R3656 T4505 T4499 arg1Of U52683,promoter
R3657 T4505 T4500 arg1Of U52683,(
R3658 T4505 T4503 arg1Of U52683,(
R3659 T4505 T4504 arg1Of U52683,GenBank
R3660 T4509 T4507 arg2Of 3A,see
R3661 T4509 T4508 arg1Of 3A,Figure
R3662 T4512 T4511 arg1Of sequences,The
R3663 T4512 T4513 arg1Of sequences,of
R3664 T4512 T4516 arg1Of sequences,were
R3665 T4512 T4517 arg1Of sequences,F1-forward
R3666 T4515 T4513 arg2Of primers,of
R3667 T4515 T4514 arg1Of primers,the
R3668 T4516 T4518 arg1Of were,:
R3669 T4517 T4516 arg2Of F1-forward,were
R3670 T4519 T4520 arg1Of 5′-TTGAGATGGAGTCTTGCTCTGT-3′,","
R3671 T4520 T4522 arg1Of ",",:
R3672 T4521 T4520 arg2Of F1-reverse,","
R3673 T4523 T4519 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,5′-TTGAGATGGAGTCTTGCTCTGT-3′
R3674 T4523 T4521 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,F1-reverse
R3675 T4523 T4524 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,(
R3676 T4523 T4528 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,;
R3677 T4526 T4524 arg2Of bp,(
R3678 T4526 T4525 arg1Of bp,341
R3679 T4527 T4524 arg3Of ),(
R3680 T4529 T4528 arg2Of F2-forward,;
R3681 T4529 T4530 arg1Of F2-forward,:
R3682 T4531 T4532 arg1Of 5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′,","
R3683 T4532 T4534 arg1Of ",",:
R3684 T4533 T4532 arg2Of F2-reverse,","
R3685 T4535 T4536 arg1Of 5′-CCAGGACCTCAGGAGGCCAGTCA-3′,(
R3686 T4538 T4536 arg2Of bp,(
R3687 T4538 T4537 arg1Of bp,474
R3688 T4539 T4536 arg3Of ),(
R3689 T4542 T4540 arg1Of conditions,The
R3690 T4542 T4541 arg1Of conditions,PCR
R3691 T4542 T4543 arg1Of conditions,were
R3692 T4542 T4544 arg2Of conditions,described
R3693 T4544 T4543 arg2Of described,were
R3694 T4544 T4545 arg1Of described,elsewhere
R3695 T4545 T4546 arg1Of elsewhere,(
R3696 T4547 T4546 arg2Of 3,(
R3697 T4548 T4546 arg3Of ),(
R3698 T4549 T4550 arg1Of PCR,was
R3699 T4549 T4551 arg2Of PCR,performed
R3700 T4551 T4550 arg2Of performed,was
R3701 T4551 T4552 arg1Of performed,with
R3702 T4555 T4552 arg2Of temperature,with
R3703 T4555 T4553 arg1Of temperature,an
R3704 T4555 T4554 arg1Of temperature,annealing
R3705 T4555 T4556 arg1Of temperature,of
R3706 T4557 T4558 arg1Of 62°C,and
R3707 T4558 T4556 arg2Of and,of
R3708 T4560 T4558 arg2Of cycles,and
R3709 T4560 T4559 arg1Of cycles,35
R3710 T4562 T4563 arg1Of DNA,was
R3711 T4562 T4564 arg2Of DNA,methylated
R3712 T4564 T4561 arg2Of methylated,When
R3713 T4564 T4563 arg2Of methylated,was
R3714 T4564 T4565 arg1Of methylated,at
R3715 T4567 T4565 arg2Of sites,at
R3716 T4567 T4566 arg1Of sites,specific
R3717 T4571 T4569 arg1Of enzymes,the
R3718 T4571 T4570 arg1Of enzymes,sensitive
R3719 T4571 T4572 arg1Of enzymes,were
R3720 T4571 T4574 arg1Of enzymes,able
R3721 T4571 T4576 arg1Of enzymes,digest
R3722 T4572 T4573 arg1Of were,not
R3723 T4572 T4579 arg1Of were,and
R3724 T4574 T4572 arg2Of able,were
R3725 T4576 T4574 arg2Of digest,able
R3726 T4576 T4575 arg1Of digest,to
R3727 T4578 T4576 arg2Of DNA,digest
R3728 T4578 T4577 arg1Of DNA,the
R3729 T4579 T4561 arg1Of and,When
R3730 T4579 T4568 arg1Of and,","
R3731 T4579 T4583 arg1Of and,;
R3732 T4580 T4581 arg1Of amplification,took
R3733 T4581 T4579 arg2Of took,and
R3734 T4582 T4581 arg2Of place,took
R3735 T4585 T4584 arg2Of case,in
R3736 T4585 T4586 arg1Of case,of
R3737 T4588 T4586 arg2Of methylation,of
R3738 T4588 T4587 arg1Of methylation,no
R3739 T4590 T4591 arg1Of DNA,was
R3740 T4590 T4592 arg2Of DNA,digested
R3741 T4592 T4591 arg2Of digested,was
R3742 T4592 T4593 arg1Of digested,and
R3743 T4593 T4583 arg2Of and,;
R3744 T4593 T4584 arg1Of and,in
R3745 T4593 T4589 arg1Of and,","
R3746 T4595 T4594 arg1Of product,no
R3747 T4595 T4596 arg1Of product,was
R3748 T4595 T4597 arg2Of product,generated
R3749 T4597 T4593 arg2Of generated,and
R3750 T4597 T4596 arg2Of generated,was
R3751 T4600 T4598 arg1Of products,The
R3752 T4600 T4599 arg1Of products,PCR
R3753 T4600 T4601 arg1Of products,were
R3754 T4600 T4602 arg2Of products,electrophoresed
R3755 T4600 T4610 arg1Of products,were
R3756 T4600 T4611 arg2Of products,stained
R3757 T4600 T4616 arg2Of products,photographed
R3758 T4602 T4601 arg2Of electrophoresed,were
R3759 T4602 T4603 arg1Of electrophoresed,on
R3760 T4602 T4609 arg1Of electrophoresed,","
R3761 T4605 T4606 arg1Of 3,%
R3762 T4608 T4603 arg2Of gel,on
R3763 T4608 T4604 arg1Of gel,a
R3764 T4608 T4605 arg1Of gel,3
R3765 T4608 T4607 arg1Of gel,agarose
R3766 T4611 T4612 arg1Of stained,with
R3767 T4611 T4615 arg1Of stained,and
R3768 T4614 T4612 arg2Of bromide,with
R3769 T4614 T4613 arg1Of bromide,ethidium
R3770 T4615 T4609 arg2Of and,","
R3771 T4615 T4610 arg2Of and,were
R3772 T4616 T4615 arg2Of photographed,and
R3773 T4618 T4617 arg1Of products,PCR
R3774 T4618 T4619 arg1Of products,were
R3775 T4618 T4620 arg2Of products,verified
R3776 T4620 T4619 arg2Of verified,were
R3777 T4623 T4620 arg1Of sequencing,verified
R3778 T4623 T4621 arg2Of sequencing,by
R3779 T4623 T4622 arg2Of sequencing,automated