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PMC:1310901 / 5129-13048 JSONTXT 18 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2710 23-27 NN denotes Cell
T2711 28-33 NN denotes lines
T2712 34-39 NN denotes K-562
T2713 39-40 -COMMA- denotes ,
T2714 41-47 NN denotes Jurkat
T2715 48-51 CC denotes and
T2716 52-57 NN denotes U-937
T2717 58-62 VB denotes were
T2718 63-71 VB denotes obtained
T2719 72-76 IN denotes from
T2720 77-80 DT denotes the
T2721 81-85 NN denotes ATCC
T2722 86-87 -LRB- denotes (
T2723 87-95 JJ denotes American
T2724 96-100 NN denotes Type
T2725 101-108 NNP denotes Culture
T2726 109-119 NNP denotes Collection
T2727 119-120 -COMMA- denotes ,
T2728 121-130 NNP denotes Rockville
T2729 130-131 -COMMA- denotes ,
T2730 132-135 NNP denotes USA
T2731 135-136 -RRB- denotes )
T2732 137-140 CC denotes and
T2733 141-145 NN denotes EM-2
T2734 145-146 -COMMA- denotes ,
T2735 147-154 NN denotes LAMA-84
T2736 154-155 -COMMA- denotes ,
T2737 156-162 NN denotes CML-T1
T2738 162-163 -COMMA- denotes ,
T2739 164-170 NN denotes BV-173
T2740 170-171 -COMMA- denotes ,
T2741 172-176 NN denotes SD-1
T2742 177-180 CC denotes and
T2743 181-190 NN denotes RPMI-8226
T2744 191-195 IN denotes from
T2745 196-199 DT denotes the
T2746 200-204 NN denotes DSMZ
T2747 205-206 -LRB- denotes (
T2748 206-214 NNP denotes Deutsche
T2749 215-223 NNP denotes Sammlung
T2750 224-227 NNP denotes von
T2751 228-243 NNP denotes Mikroorganismen
T2752 244-247 VB denotes und
T2753 248-260 NNP denotes Zellkulturen
T2754 261-265 NNP denotes GmbH
T2755 265-266 -COMMA- denotes ,
T2756 267-279 NNP denotes Braunschweig
T2757 279-280 -COMMA- denotes ,
T2758 281-288 NNP denotes Germany
T2759 288-289 -RRB- denotes )
T2760 291-294 DT denotes All
T2761 295-299 NN denotes cell
T2762 300-305 NN denotes lines
T2763 305-306 -COMMA- denotes ,
T2764 307-313 IN denotes except
T2765 314-320 NN denotes BV-173
T2766 320-321 -COMMA- denotes ,
T2767 322-326 NN denotes SD-1
T2768 327-330 CC denotes and
T2769 331-340 NN denotes RPMI-8226
T2770 340-341 -COMMA- denotes ,
T2771 342-346 VB denotes were
T2772 347-361 JJ denotes IRF-4-negative
T2862 364-368 NN denotes Cell
T2863 369-376 NN denotes culture
T2864 377-380 CC denotes and
T2865 381-392 NN denotes stimulation
T2866 393-396 DT denotes All
T2867 397-401 NN denotes cell
T2868 402-407 NN denotes lines
T2869 408-412 VB denotes were
T2870 413-423 VB denotes maintained
T2871 424-426 IN denotes at
T2872 427-428 CD denotes 5
T2873 428-429 NN denotes %
T2874 430-433 NN denotes CO2
T2875 434-436 IN denotes in
T2876 437-441 NN denotes RPMI
T2877 442-446 CD denotes 1640
T2878 447-453 NN denotes medium
T2879 454-458 IN denotes with
T2880 459-460 CD denotes 1
T2881 460-461 NN denotes %
T2882 462-471 NN denotes glutamine
T2883 472-473 -LRB- denotes (
T2884 473-482 NNP denotes Gibco/BRL
T2885 483-493 NNP denotes Eggenstein
T2886 493-494 -COMMA- denotes ,
T2887 495-502 NNP denotes Germany
T2888 502-503 -RRB- denotes )
T2889 504-516 VB denotes supplemented
T2890 517-521 IN denotes with
T2891 522-524 CD denotes 10
T2892 524-525 NN denotes %
T2893 526-531 JJ denotes fetal
T2894 532-536 NN denotes calf
T2895 537-542 NN denotes serum
T2896 543-544 -LRB- denotes (
T2897 544-553 NN denotes Gibco/BRL
T2898 553-554 -RRB- denotes )
T2899 554-555 -COMMA- denotes ,
T2900 556-557 CD denotes 1
T2901 557-558 NN denotes %
T2902 559-582 NN denotes penicillin/streptomycin
T2903 583-584 -LRB- denotes (
T2904 584-592 NNP denotes Biochrom
T2905 592-593 -COMMA- denotes ,
T2906 594-600 NNP denotes Berlin
T2907 600-601 -COMMA- denotes ,
T2908 602-609 NNP denotes Germany
T2909 609-610 -RRB- denotes )
T2910 612-616 WRB denotes When
T2911 617-626 VB denotes indicated
T2912 626-627 -COMMA- denotes ,
T2913 628-633 NN denotes cells
T2914 634-638 VB denotes were
T2915 639-646 VB denotes treated
T2916 647-651 IN denotes with
T2917 652-673 NN denotes 5-aza-2-deoxycytidine
T2918 674-675 -LRB- denotes (
T2919 675-680 NN denotes AzadC
T2920 680-681 -RRB- denotes )
T2921 682-684 CC denotes or
T2922 685-698 NN denotes 5-azacytidine
T2923 699-700 -LRB- denotes (
T2924 700-704 NN denotes AzaC
T2925 704-705 -RRB- denotes )
T2926 706-707 -LRB- denotes (
T2927 707-712 NNP denotes Sigma
T2928 712-713 -COMMA- denotes ,
T2929 714-725 NNP denotes Taufkirchen
T2930 725-726 -COMMA- denotes ,
T2931 727-734 NNP denotes Germany
T2932 734-735 -RRB- denotes )
T2933 736-739 IN denotes for
T2934 740-749 JJ denotes different
T2935 750-754 NN denotes time
T2936 755-762 NN denotes periods
T2937 764-769 VB denotes Owing
T2938 770-772 TO denotes to
T2939 773-778 PRP-DOLLAR- denotes their
T2940 779-787 JJ denotes chemical
T2941 788-799 NN denotes instability
T2942 800-805 JJ denotes fresh
T2943 806-816 NN denotes substances
T2944 817-821 VB denotes were
T2945 822-830 JJ denotes re-added
T2946 831-836 DT denotes every
T2947 837-839 CD denotes 24
T2948 840-841 NN denotes h
T3062 844-854 NN denotes Sequencing
T3063 855-857 IN denotes of
T3064 858-861 DT denotes the
T3065 862-867 NN denotes IRF-4
T3066 868-876 NN denotes promoter
T3067 877-880 IN denotes For
T3068 881-889 NN denotes analysis
T3069 890-892 IN denotes of
T3070 893-896 DT denotes the
T3071 897-902 NN denotes IRF-4
T3072 903-911 NN denotes promoter
T3073 912-918 NN denotes region
T3074 919-922 IN denotes for
T3075 923-932 JJ denotes permanent
T3076 933-944 NN denotes aberrations
T3077 945-949 JJ denotes such
T3078 950-952 IN denotes as
T3079 953-973 NN denotes insertions/deletions
T3080 974-976 CC denotes or
T3081 977-985 NN denotes mutation
T3082 985-986 -COMMA- denotes ,
T3083 987-989 PRP denotes we
T3084 990-1003 VB denotes PCR-amplified
T3085 1004-1007 CD denotes two
T3086 1008-1017 NN denotes fragments
T3087 1018-1022 IN denotes from
T3088 1023-1030 JJ denotes genomic
T3089 1031-1034 NN denotes DNA
T3090 1034-1035 -COMMA- denotes ,
T3091 1036-1041 WDT denotes which
T3092 1042-1045 VB denotes was
T3093 1046-1055 VB denotes extracted
T3094 1056-1060 IN denotes from
T3095 1061-1069 VB denotes depicted
T3096 1070-1074 NN denotes cell
T3097 1075-1080 NN denotes lines
T3098 1081-1085 IN denotes with
T3099 1086-1087 DT denotes a
T3100 1088-1098 JJ denotes commercial
T3101 1099-1102 NN denotes kit
T3102 1103-1104 -LRB- denotes (
T3103 1104-1110 NN denotes Qiagen
T3104 1110-1111 -COMMA- denotes ,
T3105 1112-1117 NNP denotes Hilde
T3106 1117-1118 -COMMA- denotes ,
T3107 1119-1126 NNP denotes Germany
T3108 1126-1127 -RRB- denotes )
T3109 1128-1130 IN denotes as
T3110 1131-1142 VB denotes recommended
T3111 1144-1147 DT denotes The
T3112 1148-1155 NN denotes primers
T3113 1156-1160 VB denotes were
T3114 1161-1170 JJ denotes 1-forward
T3115 1170-1171 -COLON- denotes :
T3116 1172-1200 JJ denotes 5′-TTGAGATGGAGTCTTGCTCTGT-3′
T3117 1200-1201 -COMMA- denotes ,
T3118 1202-1211 JJ denotes 1-reverse
T3119 1211-1212 -COLON- denotes :
T3120 1213-1242 NN denotes 5′-CCAGGACCTCAGGAGGCCAGTCA-3′
T3121 1242-1243 -COLON- denotes ;
T3122 1244-1253 CD denotes 2-forward
T3123 1253-1254 -COLON- denotes :
T3124 1255-1286 JJ denotes 5′-AGCGGTGAAACTGAGAGTGCGAGGT-3′
T3125 1286-1287 -COMMA- denotes ,
T3126 1288-1297 JJ denotes 2-reverse
T3127 1297-1298 -COLON- denotes :
T3128 1299-1325 NN denotes 5′-GCCACATCGCTGCAGTTTAG-3′
T3129 1327-1330 DT denotes The
T3130 1331-1339 NN denotes products
T3131 1340-1344 VB denotes were
T3132 1345-1351 VB denotes cloned
T3133 1352-1356 IN denotes with
T3134 1357-1360 DT denotes the
T3135 1361-1366 NN denotes ‘TOPO
T3136 1367-1369 NN denotes TA
T3137 1370-1377 NN denotes cloning
T3138 1378-1382 NN denotes kit’
T3139 1383-1384 -LRB- denotes (
T3140 1384-1394 NN denotes Invitrogen
T3141 1394-1395 -COMMA- denotes ,
T3142 1396-1405 NN denotes Groningen
T3143 1405-1406 -COMMA- denotes ,
T3144 1407-1410 DT denotes The
T3145 1411-1422 NNP denotes Netherlands
T3146 1422-1423 -RRB- denotes )
T3147 1425-1430 IN denotes After
T3148 1431-1440 JJ denotes bacterial
T3149 1441-1454 NN denotes amplification
T3150 1455-1457 IN denotes of
T3151 1458-1461 DT denotes the
T3152 1462-1468 VB denotes cloned
T3153 1469-1472 NN denotes PCR
T3154 1473-1482 NN denotes fragments
T3155 1483-1485 IN denotes by
T3156 1486-1494 JJ denotes standard
T3157 1495-1505 NN denotes procedures
T3158 1505-1506 -COMMA- denotes ,
T3159 1507-1509 IN denotes at
T3160 1510-1515 JJ denotes least
T3161 1516-1521 CD denotes three
T3162 1522-1528 NN denotes clones
T3163 1529-1533 IN denotes from
T3164 1534-1538 DT denotes each
T3165 1539-1545 NN denotes sample
T3166 1546-1550 VB denotes were
T3167 1551-1560 VB denotes sequenced
T3168 1561-1565 IN denotes with
T3169 1566-1568 DT denotes an
T3170 1569-1578 VB denotes automated
T3171 1579-1588 NN denotes sequencer
T3172 1589-1590 -LRB- denotes (
T3173 1590-1593 NNP denotes ABI
T3174 1594-1599 NNP denotes Prism
T3175 1600-1603 CD denotes 377
T3176 1603-1604 -COMMA- denotes ,
T3177 1605-1612 NNP denotes Applied
T3178 1613-1624 NNP denotes Bio-systems
T3179 1624-1625 -COMMA- denotes ,
T3180 1626-1632 NNP denotes Foster
T3181 1633-1637 NNP denotes City
T3182 1637-1638 -COMMA- denotes ,
T3183 1639-1642 NNP denotes USA
T3184 1642-1643 -RRB- denotes )
T3185 1644-1646 IN denotes as
T3186 1647-1658 VB denotes recommended
T3187 1659-1661 IN denotes by
T3188 1662-1665 DT denotes the
T3189 1666-1678 NN denotes manufacturer
T3450 1681-1691 NN denotes Expression
T3451 1692-1700 NN denotes analysis
T3452 1701-1703 TO denotes To
T3453 1704-1711 VB denotes analyze
T3454 1712-1715 DT denotes the
T3455 1716-1721 NN denotes IRF-4
T3456 1722-1737 JJ denotes transcriptional
T3457 1738-1743 NN denotes level
T3458 1743-1744 -COMMA- denotes ,
T3459 1745-1748 NN denotes RNA
T3460 1749-1752 VB denotes was
T3461 1753-1762 VB denotes extracted
T3462 1763-1767 IN denotes from
T3463 1768-1773 NN denotes cells
T3464 1774-1779 VB denotes using
T3465 1780-1783 DT denotes the
T3466 1784-1794 JJ denotes commercial
T3467 1795-1805 NN denotes RNAzol-kit
T3468 1806-1807 -LRB- denotes (
T3469 1807-1813 NNP denotes Paesel
T3470 1813-1814 -COMMA- denotes ,
T3471 1815-1824 NNP denotes Frankfurt
T3472 1824-1825 -COMMA- denotes ,
T3473 1826-1833 NNP denotes Germany
T3474 1833-1834 -RRB- denotes )
T3475 1836-1838 DT denotes An
T3476 1839-1846 NN denotes aliquot
T3477 1847-1849 IN denotes of
T3478 1850-1851 CD denotes 1
T3479 1852-1854 NN denotes μg
T3480 1855-1860 JJ denotes total
T3481 1861-1864 NN denotes RNA
T3482 1865-1868 VB denotes was
T3483 1869-1873 VB denotes used
T3484 1874-1877 IN denotes for
T3485 1878-1882 NN denotes cDNA
T3486 1883-1892 NN denotes synthesis
T3487 1893-1895 IN denotes as
T3488 1896-1905 VB denotes described
T3489 1906-1916 RB denotes previously
T3490 1917-1918 -LRB- denotes (
T3491 1918-1920 CD denotes 27
T3492 1920-1921 -RRB- denotes )
T3493 1923-1926 NN denotes RNA
T3494 1927-1937 NN denotes expression
T3495 1938-1946 NN denotes analysis
T3496 1947-1950 IN denotes for
T3497 1951-1956 NN denotes IRF-4
T3498 1957-1960 CC denotes and
T3499 1961-1964 DT denotes the
T3500 1965-1974 NN denotes reference
T3501 1975-1979 NN denotes gene
T3502 1980-1987 NN denotes β-actin
T3503 1988-1991 VB denotes was
T3504 1992-1999 VB denotes carried
T3505 2000-2003 RP denotes out
T3506 2004-2006 IN denotes by
T3507 2007-2024 JJ denotes semi-quantitative
T3508 2025-2028 NN denotes PCR
T3509 2029-2031 IN denotes as
T3510 2032-2041 VB denotes described
T3511 2042-2052 RB denotes previously
T3512 2053-2054 -LRB- denotes (
T3513 2054-2058 CD denotes 3,27
T3514 2058-2059 -RRB- denotes )
T3515 2061-2064 NN denotes PCR
T3516 2065-2073 NN denotes products
T3517 2074-2078 VB denotes were
T3518 2079-2087 VB denotes verified
T3519 2088-2090 IN denotes by
T3520 2091-2100 VB denotes automated
T3521 2101-2111 NN denotes sequencing
T3522 2113-2116 NN denotes PCR
T3523 2117-2124 NN denotes primers
T3524 2125-2128 CC denotes and
T3525 2129-2139 NN denotes conditions
T3526 2140-2143 IN denotes for
T3527 2144-2154 NN denotes expression
T3528 2155-2163 NN denotes analysis
T3529 2164-2166 IN denotes of
T3530 2167-2171 NN denotes DNMT
T3531 2172-2174 CC denotes or
T3532 2175-2178 NN denotes MBP
T3533 2179-2180 -LRB- denotes (
T3534 2180-2185 NN denotes DNMT1
T3535 2186-2192 NN denotes DNMT3A
T3536 2192-2193 -COMMA- denotes ,
T3537 2194-2200 NN denotes DNMT3B
T3538 2200-2201 -COMMA- denotes ,
T3539 2202-2206 NN denotes MeCP
T3540 2206-2207 -COMMA- denotes ,
T3541 2208-2212 NN denotes MBD1
T3542 2212-2213 -COMMA- denotes ,
T3543 2214-2218 NN denotes MBD2
T3544 2219-2222 CC denotes and
T3545 2223-2227 NN denotes MBD4
T3546 2227-2228 -RRB- denotes )
T3547 2229-2233 VB denotes were
T3548 2234-2243 VB denotes published
T3549 2244-2253 RB denotes elsewhere
T3550 2254-2255 -LRB- denotes (
T3551 2255-2257 CD denotes 28
T3552 2257-2258 -RRB- denotes )
T3553 2260-2263 IN denotes For
T3554 2264-2272 NN denotes analysis
T3555 2273-2275 IN denotes of
T3556 2276-2281 NN denotes IRF-4
T3557 2282-2289 NN denotes protein
T3558 2290-2300 NN denotes expression
T3559 2300-2301 -COMMA- denotes ,
T3560 2302-2303 DT denotes a
T3561 2304-2312 JJ denotes standard
T3562 2313-2327 NN denotes immunoblotting
T3563 2328-2333 NN denotes assay
T3564 2334-2337 VB denotes was
T3565 2338-2347 VB denotes performed
T3566 2348-2350 IN denotes as
T3567 2351-2360 VB denotes described
T3568 2361-2371 RB denotes previously
T3569 2372-2373 -LRB- denotes (
T3570 2373-2375 CD denotes 29
T3571 2375-2376 -RRB- denotes )
T3572 2378-2385 RB denotes Briefly
T3573 2385-2386 -COMMA- denotes ,
T3574 2387-2394 NN denotes protein
T3575 2395-2402 NN denotes lysates
T3576 2403-2407 VB denotes were
T3577 2408-2417 VB denotes generated
T3578 2418-2420 IN denotes by
T3579 2421-2431 VB denotes incubating
T3580 2432-2433 CD denotes 1
T3581 2434-2435 NN denotes ×
T3582 2436-2439 CD denotes 106
T3583 2440-2445 NN denotes cells
T3584 2446-2448 IN denotes in
T3585 2449-2452 CD denotes 100
T3586 2453-2455 NN denotes µl
T3587 2456-2460 NN denotes RIPA
T3588 2461-2467 NN denotes buffer
T3589 2468-2469 -LRB- denotes (
T3590 2469-2470 CD denotes 1
T3591 2470-2471 NN denotes %
T3592 2472-2477 NN denotes NP-40
T3593 2477-2478 -COMMA- denotes ,
T3594 2479-2482 CD denotes 0.5
T3595 2482-2483 NN denotes %
T3596 2484-2503 NN denotes sodiumdesoxycholate
T3597 2503-2504 -COMMA- denotes ,
T3598 2505-2508 CD denotes 0.1
T3599 2508-2509 NN denotes %
T3600 2510-2513 NN denotes SDS
T3601 2513-2514 -COMMA- denotes ,
T3602 2515-2518 CD denotes 100
T3603 2519-2524 NN denotes µg/ml
T3604 2525-2545 NN denotes phenylmethylsulfonyl
T3605 2546-2554 NN denotes fluoride
T3606 2554-2555 -COMMA- denotes ,
T3607 2556-2558 CD denotes 10
T3608 2559-2564 NN denotes µl/ml
T3609 2565-2588 NN denotes protease-inhibitory-mix
T3610 2588-2589 -COMMA- denotes ,
T3611 2590-2591 CD denotes 1
T3612 2592-2599 NN denotes µmol/ml
T3613 2600-2619 NN denotes sodiumorthovanadate
T3614 2620-2622 IN denotes in
T3615 2623-2641 JJ denotes phosphate-buffered
T3616 2642-2648 NN denotes saline
T3617 2648-2649 -RRB- denotes )
T3618 2650-2653 IN denotes for
T3619 2654-2656 CD denotes 30
T3620 2657-2660 NN denotes min
T3621 2661-2663 IN denotes on
T3622 2664-2667 NN denotes ice
T3623 2669-2674 IN denotes After
T3624 2675-2689 NN denotes centrifugation
T3625 2689-2690 -COMMA- denotes ,
T3626 2691-2698 NN denotes protein
T3627 2699-2712 NN denotes concentration
T3628 2713-2715 IN denotes of
T3629 2716-2719 DT denotes the
T3630 2720-2731 NN denotes supernatant
T3631 2732-2735 VB denotes was
T3632 2736-2746 VB denotes determined
T3633 2747-2749 IN denotes by
T3634 2750-2760 NN denotes BCA-method
T3635 2761-2762 -LRB- denotes (
T3636 2762-2768 NNP denotes Pierce
T3637 2768-2769 -COMMA- denotes ,
T3638 2770-2778 NNP denotes Rockford
T3639 2778-2779 -COMMA- denotes ,
T3640 2780-2782 NN denotes IL
T3641 2782-2783 -RRB- denotes )
T3642 2784-2786 IN denotes as
T3643 2787-2798 VB denotes recommended
T3644 2800-2807 NN denotes Protein
T3645 2808-2815 NN denotes lysates
T3646 2816-2817 -LRB- denotes (
T3647 2817-2823 CD denotes 70–100
T3648 2824-2826 NN denotes µg
T3649 2826-2827 -RRB- denotes )
T3650 2828-2832 VB denotes were
T3651 2833-2848 VB denotes electrophoresed
T3652 2849-2851 IN denotes on
T3653 2852-2866 NN denotes polyacrylamide
T3654 2867-2871 NN denotes gels
T3655 2872-2875 CC denotes and
T3656 2876-2887 VB denotes transferred
T3657 2888-2890 TO denotes to
T3658 2891-2892 DT denotes a
T3659 2893-2906 NN denotes PVDF-membrane
T3660 2907-2908 -LRB- denotes (
T3661 2908-2917 NNP denotes Immobilon
T3662 2918-2919 NNP denotes P
T3663 2919-2920 -COMMA- denotes ,
T3664 2921-2925 CD denotes 0.45
T3665 2926-2928 NN denotes µm
T3666 2928-2929 -COLON- denotes ;
T3667 2930-2939 NNP denotes Millipore
T3668 2939-2940 -COMMA- denotes ,
T3669 2941-2949 NNP denotes Eschborn
T3670 2949-2950 -COMMA- denotes ,
T3671 2951-2958 NNP denotes Germany
T3672 2958-2959 -RRB- denotes )
T3673 2961-2970 NN denotes Membranes
T3674 2971-2975 VB denotes were
T3675 2976-2983 VB denotes blocked
T3676 2984-2988 IN denotes with
T3677 2989-2992 CD denotes 2.5
T3678 2992-2993 NN denotes %
T3679 2994-3002 VB denotes blocking
T3680 3003-3010 NN denotes reagent
T3681 3011-3012 -LRB- denotes (
T3682 3012-3022 NNP denotes Boehringer
T3683 3023-3031 NNP denotes Mannheim
T3684 3031-3032 -COMMA- denotes ,
T3685 3033-3040 NNP denotes Germany
T3686 3040-3041 -RRB- denotes )
T3687 3042-3044 IN denotes in
T3688 3045-3049 NN denotes TBST
T3689 3050-3056 NN denotes buffer
T3690 3057-3058 -LRB- denotes (
T3691 3058-3062 CD denotes 4.44
T3692 3063-3066 NN denotes g/l
T3693 3067-3075 NN denotes Tris–HCL
T3694 3075-3076 -COMMA- denotes ,
T3695 3077-3081 CD denotes 2.65
T3696 3082-3085 NN denotes g/l
T3697 3086-3092 NN denotes TrisOH
T3698 3092-3093 -COMMA- denotes ,
T3699 3094-3098 CD denotes 8.07
T3700 3099-3102 NN denotes g/l
T3701 3103-3107 NN denotes NaCl
T3702 3107-3108 -COMMA- denotes ,
T3703 3109-3112 CD denotes 0.2
T3704 3113-3116 NN denotes g/l
T3705 3117-3120 NN denotes KCl
T3706 3121-3124 CC denotes and
T3707 3125-3128 CD denotes 500
T3708 3129-3133 VB denotes µl/l
T3709 3134-3142 CD denotes Tween-20
T3710 3143-3145 IN denotes in
T3711 3146-3149 NN denotes H2O
T3712 3149-3150 -RRB- denotes )
T3713 3151-3154 CC denotes and
T3714 3155-3167 RB denotes subsequently
T3715 3168-3177 VB denotes incubated
T3716 3178-3182 IN denotes with
T3717 3183-3190 JJ denotes primary
T3718 3191-3199 NN denotes antibody
T3719 3200-3202 IN denotes as
T3720 3203-3212 VB denotes indicated
T3721 3213-3216 CC denotes and
T3722 3217-3228 JJ denotes horseradish
T3723 3229-3250 JJ denotes peroxidase-conjugated
T3724 3251-3260 JJ denotes secondary
T3725 3261-3269 NN denotes antibody
T3726 3269-3270 -COMMA- denotes ,
T3727 3271-3281 JJ denotes anti-mouse
T3728 3282-3284 CC denotes or
T3729 3285-3294 JJ denotes anti-goat
T3730 3295-3298 NN denotes IgG
T3731 3299-3300 -LRB- denotes (
T3732 3300-3304 NNP denotes DAKO
T3733 3304-3305 -COMMA- denotes ,
T3734 3306-3313 NNP denotes Hamburg
T3735 3313-3314 -COMMA- denotes ,
T3736 3315-3322 NNP denotes Germany
T3737 3322-3323 -RRB- denotes )
T3738 3323-3324 -COMMA- denotes ,
T3739 3325-3337 RB denotes respectively
T3740 3339-3342 DT denotes The
T3741 3343-3352 NN denotes membranes
T3742 3353-3357 VB denotes were
T3743 3358-3362 RB denotes then
T3744 3363-3372 VB denotes developed
T3745 3373-3377 IN denotes with
T3746 3378-3380 DT denotes an
T3747 3381-3384 NN denotes ECL
T3748 3385-3394 NN denotes detection
T3749 3395-3398 NN denotes kit
T3750 3399-3400 -LRB- denotes (
T3751 3400-3408 NNP denotes Amersham
T3752 3409-3418 NNP denotes Pharmacia
T3753 3419-3426 NNP denotes Biotech
T3754 3426-3427 -COMMA- denotes ,
T3755 3428-3436 NNP denotes Freiburg
T3756 3436-3437 -COMMA- denotes ,
T3757 3438-3445 NNP denotes Germany
T3758 3445-3446 -RRB- denotes )
T3759 3448-3451 DT denotes The
T3760 3452-3459 JJ denotes primary
T3761 3460-3470 NN denotes antibodies
T3762 3471-3475 VB denotes were
T3763 3476-3480 NN denotes goat
T3764 3481-3497 NN denotes anti-IRF-4/ICSAT
T3765 3498-3499 -LRB- denotes (
T3766 3499-3503 NN denotes M-17
T3767 3503-3504 -RRB- denotes )
T3768 3505-3506 -LRB- denotes (
T3769 3506-3511 NNP denotes Santa
T3770 3512-3516 NNP denotes Cruz
T3771 3517-3530 NNP denotes Biotechnology
T3772 3530-3531 -COMMA- denotes ,
T3773 3532-3537 NNP denotes Santa
T3774 3538-3542 NNP denotes Cruz
T3775 3542-3543 -COMMA- denotes ,
T3776 3544-3546 NN denotes CA
T3777 3546-3547 -RRB- denotes )
T3778 3548-3551 CC denotes and
T3779 3552-3557 NN denotes mouse
T3780 3558-3570 JJ denotes anti-β-actin
T3781 3571-3572 -LRB- denotes (
T3782 3572-3577 NN denotes AC-74
T3783 3577-3578 -RRB- denotes )
T3784 3579-3580 -LRB- denotes (
T3785 3580-3585 NN denotes Sigma
T3786 3585-3586 -RRB- denotes )
T4371 3589-3609 JJ denotes Methylation-specific
T4372 3610-3631 NN denotes restriction-PCR-assay
T4373 3632-3635 NN denotes DNA
T4374 3636-3639 VB denotes was
T4375 3640-3649 VB denotes extracted
T4376 3650-3654 IN denotes with
T4377 3655-3656 DT denotes a
T4378 3657-3667 JJ denotes commercial
T4379 3668-3671 NN denotes kit
T4380 3672-3673 -LRB- denotes (
T4381 3673-3679 NN denotes Qiagen
T4382 3679-3680 -RRB- denotes )
T4383 3681-3683 IN denotes as
T4384 3684-3695 VB denotes recommended
T4385 3697-3702 IN denotes Since
T4386 3703-3706 DT denotes the
T4387 3707-3718 NN denotes restriction
T4388 3719-3726 NN denotes ability
T4389 3727-3729 IN denotes of
T4390 3730-3737 JJ denotes several
T4391 3738-3751 NN denotes endonucleases
T4392 3752-3754 VB denotes is
T4393 3755-3764 VB denotes inhibited
T4394 3765-3767 IN denotes by
T4395 3768-3779 NN denotes methylation
T4396 3780-3782 IN denotes of
T4397 3783-3788 PRP-DOLLAR- denotes their
T4398 3789-3795 NN denotes target
T4399 3796-3804 NN denotes sequence
T4400 3804-3805 -COMMA- denotes ,
T4401 3806-3808 PRP denotes we
T4402 3809-3813 VB denotes used
T4403 3814-3835 JJ denotes methylation-sensitive
T4404 3836-3843 NN denotes enzymes
T4405 3844-3849 NN denotes HpaII
T4406 3850-3853 CC denotes and
T4407 3854-3871 NN denotes HaeII-isochizomer
T4408 3872-3880 NN denotes Bsp143II
T4409 3881-3884 CC denotes and
T4410 3885-3893 NN denotes Bsh1236I
T4411 3894-3895 -LRB- denotes (
T4412 3895-3898 NNP denotes MBI
T4413 3899-3908 NNP denotes Fermentas
T4414 3908-3909 -COMMA- denotes ,
T4415 3910-3912 NNP denotes St
T4416 3913-3921 NNP denotes Leon-Rot
T4417 3921-3922 -COMMA- denotes ,
T4418 3923-3930 NNP denotes Germany
T4419 3930-3931 -RRB- denotes )
T4420 3932-3933 -LRB- denotes (
T4421 3933-3938 CD denotes 20,24
T4422 3938-3939 -RRB- denotes )
T4423 3941-3943 IN denotes As
T4424 3944-3951 VB denotes control
T4425 3952-3955 DT denotes the
T4426 3956-3977 JJ denotes methylation-resistant
T4427 3978-3984 NN denotes enzyme
T4428 3985-3989 NN denotes MspI
T4429 3990-3993 CC denotes and
T4430 3994-3996 DT denotes an
T4431 3997-4003 NN denotes enzyme
T4432 4004-4008 IN denotes with
T4433 4009-4011 DT denotes no
T4434 4012-4023 NN denotes recognition
T4435 4024-4028 NN denotes site
T4436 4029-4031 IN denotes in
T4437 4032-4035 DT denotes the
T4438 4036-4042 NN denotes target
T4439 4043-4051 NN denotes promoter
T4440 4051-4052 -COMMA- denotes ,
T4441 4053-4058 NN denotes EcoRI
T4442 4058-4059 -COMMA- denotes ,
T4443 4060-4064 VB denotes were
T4444 4065-4069 VB denotes used
T4445 4071-4074 NN denotes DNA
T4446 4075-4076 -LRB- denotes (
T4447 4076-4079 CD denotes 0.8
T4448 4080-4082 NN denotes µg
T4449 4082-4083 -RRB- denotes )
T4450 4084-4087 VB denotes was
T4451 4088-4096 VB denotes digested
T4452 4097-4099 IN denotes by
T4453 4100-4102 CD denotes 40
T4454 4103-4104 NN denotes U
T4455 4105-4108 DT denotes the
T4456 4109-4119 JJ denotes respective
T4457 4120-4126 NN denotes enzyme
T4458 4127-4130 IN denotes for
T4459 4131-4132 CD denotes 6
T4460 4133-4134 NN denotes h
T4461 4135-4138 CC denotes and
T4462 4138-4139 -COMMA- denotes ,
T4463 4140-4142 TO denotes to
T4464 4143-4149 VB denotes ensure
T4465 4150-4158 JJ denotes complete
T4466 4159-4167 NN denotes cleavage
T4467 4167-4168 -COMMA- denotes ,
T4468 4169-4179 JJ denotes additional
T4469 4180-4182 CD denotes 20
T4470 4183-4184 NN denotes U
T4471 4185-4188 IN denotes for
T4472 4189-4191 CD denotes 16
T4473 4192-4193 NN denotes h
T4474 4195-4205 RB denotes Thereafter
T4475 4206-4209 CD denotes 100
T4476 4210-4212 NN denotes ng
T4477 4213-4215 IN denotes of
T4478 4216-4224 JJ denotes digested
T4479 4225-4228 NN denotes DNA
T4480 4229-4232 VB denotes was
T4481 4233-4237 VB denotes used
T4482 4238-4240 TO denotes to
T4483 4241-4242 DT denotes a
T4484 4243-4246 NN denotes PCR
T4485 4247-4260 NN denotes amplification
T4486 4261-4263 IN denotes of
T4487 4264-4267 CD denotes two
T4488 4268-4277 NN denotes fragments
T4489 4278-4279 -LRB- denotes (
T4490 4279-4281 NN denotes F1
T4491 4282-4285 CC denotes and
T4492 4286-4288 NN denotes F2
T4493 4288-4289 -RRB- denotes )
T4494 4290-4298 VB denotes spanning
T4495 4299-4303 NN denotes part
T4496 4304-4306 IN denotes of
T4497 4307-4310 DT denotes the
T4498 4311-4316 NN denotes IRF-4
T4499 4317-4325 NN denotes promoter
T4500 4326-4327 -LRB- denotes (
T4501 4327-4329 CD denotes 30
T4502 4329-4330 -RRB- denotes )
T4503 4331-4332 -LRB- denotes (
T4504 4332-4339 NN denotes GenBank
T4505 4340-4346 NN denotes U52683
T4506 4346-4347 -COLON- denotes ;
T4507 4348-4351 VB denotes see
T4508 4352-4358 NN denotes Figure
T4509 4359-4361 NN denotes 3A
T4510 4361-4362 -RRB- denotes )
T4511 4364-4367 DT denotes The
T4512 4368-4377 NN denotes sequences
T4513 4378-4380 IN denotes of
T4514 4381-4384 DT denotes the
T4515 4385-4392 NN denotes primers
T4516 4393-4397 VB denotes were
T4517 4398-4408 JJ denotes F1-forward
T4518 4408-4409 -COLON- denotes :
T4519 4410-4438 JJ denotes 5′-TTGAGATGGAGTCTTGCTCTGT-3′
T4520 4438-4439 -COMMA- denotes ,
T4521 4440-4450 JJ denotes F1-reverse
T4522 4450-4451 -COLON- denotes :
T4523 4452-4480 NN denotes ATCACTTCCAGACTTCAGTTCACCT-3′
T4524 4481-4482 -LRB- denotes (
T4525 4482-4485 CD denotes 341
T4526 4486-4488 NN denotes bp
T4527 4488-4489 -RRB- denotes )
T4528 4489-4490 -COLON- denotes ;
T4529 4491-4501 JJ denotes F2-forward
T4530 4501-4502 -COLON- denotes :
T4531 4503-4534 JJ denotes 5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′
T4532 4534-4535 -COMMA- denotes ,
T4533 4536-4546 JJ denotes F2-reverse
T4534 4546-4547 -COLON- denotes :
T4535 4548-4577 NN denotes 5′-CCAGGACCTCAGGAGGCCAGTCA-3′
T4536 4578-4579 -LRB- denotes (
T4537 4579-4582 CD denotes 474
T4538 4583-4585 NN denotes bp
T4539 4585-4586 -RRB- denotes )
T4540 4588-4591 DT denotes The
T4541 4592-4595 NN denotes PCR
T4542 4596-4606 NN denotes conditions
T4543 4607-4611 VB denotes were
T4544 4612-4621 VB denotes described
T4545 4622-4631 RB denotes elsewhere
T4546 4632-4633 -LRB- denotes (
T4547 4633-4634 CD denotes 3
T4548 4634-4635 -RRB- denotes )
T4549 4637-4640 NN denotes PCR
T4550 4641-4644 VB denotes was
T4551 4645-4654 VB denotes performed
T4552 4655-4659 IN denotes with
T4553 4660-4662 DT denotes an
T4554 4663-4672 JJ denotes annealing
T4555 4673-4684 NN denotes temperature
T4556 4685-4687 IN denotes of
T4557 4688-4692 NN denotes 62°C
T4558 4693-4696 CC denotes and
T4559 4697-4699 CD denotes 35
T4560 4700-4706 NN denotes cycles
T4561 4708-4712 WRB denotes When
T4562 4713-4716 NN denotes DNA
T4563 4717-4720 VB denotes was
T4564 4721-4731 VB denotes methylated
T4565 4732-4734 IN denotes at
T4566 4735-4743 JJ denotes specific
T4567 4744-4749 NN denotes sites
T4568 4749-4750 -COMMA- denotes ,
T4569 4751-4754 DT denotes the
T4570 4755-4764 JJ denotes sensitive
T4571 4765-4772 NN denotes enzymes
T4572 4773-4777 VB denotes were
T4573 4778-4781 RB denotes not
T4574 4782-4786 JJ denotes able
T4575 4787-4789 TO denotes to
T4576 4790-4796 VB denotes digest
T4577 4797-4800 DT denotes the
T4578 4801-4804 NN denotes DNA
T4579 4805-4808 CC denotes and
T4580 4809-4822 NN denotes amplification
T4581 4823-4827 VB denotes took
T4582 4828-4833 NN denotes place
T4583 4833-4834 -COLON- denotes ;
T4584 4835-4837 IN denotes in
T4585 4838-4842 NN denotes case
T4586 4843-4845 IN denotes of
T4587 4846-4848 DT denotes no
T4588 4849-4860 NN denotes methylation
T4589 4860-4861 -COMMA- denotes ,
T4590 4862-4865 NN denotes DNA
T4591 4866-4869 VB denotes was
T4592 4870-4878 VB denotes digested
T4593 4879-4882 CC denotes and
T4594 4883-4885 DT denotes no
T4595 4886-4893 NN denotes product
T4596 4894-4897 VB denotes was
T4597 4898-4907 VB denotes generated
T4598 4909-4912 DT denotes The
T4599 4913-4916 NN denotes PCR
T4600 4917-4925 NN denotes products
T4601 4926-4930 VB denotes were
T4602 4931-4946 VB denotes electrophoresed
T4603 4947-4949 IN denotes on
T4604 4950-4951 DT denotes a
T4605 4952-4953 CD denotes 3
T4606 4953-4954 NN denotes %
T4607 4955-4962 NN denotes agarose
T4608 4963-4966 NN denotes gel
T4609 4966-4967 -COMMA- denotes ,
T4610 4968-4972 VB denotes were
T4611 4973-4980 VB denotes stained
T4612 4981-4985 IN denotes with
T4613 4986-4994 NN denotes ethidium
T4614 4995-5002 NN denotes bromide
T4615 5003-5006 CC denotes and
T4616 5007-5019 VB denotes photographed
T4617 5021-5024 NN denotes PCR
T4618 5025-5033 NN denotes products
T4619 5034-5038 VB denotes were
T4620 5039-5047 VB denotes verified
T4621 5048-5050 IN denotes by
T4622 5051-5060 VB denotes automated
T4623 5061-5071 NN denotes sequencing
T4943 5074-5083 NN denotes Bisulfite
T4944 5084-5093 NN denotes treatment
T4945 5094-5097 NN denotes DNA
T4946 5098-5101 VB denotes was
T4947 5102-5111 VB denotes extracted
T4948 5112-5114 IN denotes as
T4949 5115-5124 VB denotes described
T4950 5125-5130 RB denotes above
T4951 5132-5141 NN denotes Bisulfite
T4952 5142-5151 NN denotes treatment
T4953 5152-5154 IN denotes of
T4954 5155-5158 NN denotes DNA
T4955 5158-5159 -COMMA- denotes ,
T4956 5160-5167 VB denotes leading
T4957 5168-5170 TO denotes to
T4958 5171-5181 NN denotes conversion
T4959 5182-5184 IN denotes of
T4960 5185-5197 JJ denotes unmethylated
T4961 5198-5206 NN denotes cytosine
T4962 5207-5209 TO denotes to
T4963 5210-5216 NN denotes uracil
T4964 5217-5225 NN denotes residues
T4965 5226-5229 CC denotes and
T4966 5230-5232 DT denotes no
T4967 5233-5239 NN denotes change
T4968 5240-5242 IN denotes of
T4969 5243-5253 VB denotes methylated
T4970 5254-5262 NN denotes cytosine
T4971 5263-5271 NN denotes residues
T4972 5271-5272 -COMMA- denotes ,
T4973 5273-5276 VB denotes was
T4974 5277-5286 VB denotes performed
T4975 5287-5289 IN denotes as
T4976 5290-5299 VB denotes described
T4977 5300-5302 IN denotes as
T4978 5303-5310 VB denotes follows
T4979 5312-5319 RB denotes Briefly
T4980 5319-5320 -COMMA- denotes ,
T4981 5321-5322 CD denotes 1
T4982 5323-5325 NN denotes µg
T4983 5326-5328 IN denotes of
T4984 5329-5332 NN denotes DNA
T4985 5333-5336 CC denotes and
T4986 5337-5338 CD denotes 2
T4987 5339-5341 NN denotes µg
T4988 5342-5344 IN denotes of
T4989 5345-5349 NN denotes poly
T4990 5349-5350 -LRB- denotes (
T4991 5350-5355 NN denotes dA–dT
T4992 5355-5356 -RRB- denotes )
T4993 5356-5357 -LRB- denotes (
T4994 5357-5361 NN denotes poly
T4995 5361-5362 -LRB- denotes (
T4996 5362-5367 NN denotes dA–dT
T4997 5367-5368 -RRB- denotes )
T4998 5369-5379 NN denotes copolymers
T4999 5380-5381 -LRB- denotes (
T5000 5381-5389 NNP denotes Amersham
T5001 5390-5399 NNP denotes Pharmacia
T5002 5400-5407 NNP denotes Biotech
T5003 5407-5408 -RRB- denotes )
T5004 5409-5413 VB denotes were
T5005 5414-5425 VB denotes denaturated
T5006 5426-5429 IN denotes for
T5007 5430-5432 CD denotes 20
T5008 5433-5436 NN denotes min
T5009 5437-5439 IN denotes at
T5010 5440-5444 NN denotes 42°C
T5011 5445-5447 IN denotes in
T5012 5448-5451 CD denotes 0.3
T5013 5452-5453 NN denotes M
T5014 5454-5458 NN denotes NaOH
T5015 5459-5461 IN denotes in
T5016 5462-5463 DT denotes a
T5017 5464-5470 NN denotes volume
T5018 5471-5473 IN denotes of
T5019 5474-5476 CD denotes 50
T5020 5477-5479 NN denotes µl
T5021 5481-5486 JJ denotes Fresh
T5022 5487-5496 NN denotes solutions
T5023 5497-5499 IN denotes of
T5024 5500-5502 CD denotes 30
T5025 5503-5505 NN denotes µl
T5026 5506-5508 IN denotes of
T5027 5509-5511 CD denotes 10
T5028 5512-5514 NN denotes mM
T5029 5515-5526 NN denotes hydrochinon
T5030 5527-5528 -LRB- denotes (
T5031 5528-5533 NN denotes Sigma
T5032 5533-5534 -RRB- denotes )
T5033 5535-5538 CC denotes and
T5034 5539-5542 CD denotes 530
T5035 5543-5545 NN denotes µl
T5036 5546-5548 IN denotes of
T5037 5549-5550 CD denotes 3
T5038 5551-5552 NN denotes M
T5039 5553-5559 NN denotes sodium
T5040 5560-5569 NN denotes bisulfite
T5041 5570-5571 -LRB- denotes (
T5042 5571-5573 NN denotes pH
T5043 5574-5577 CD denotes 5.0
T5044 5577-5578 -COLON- denotes ;
T5045 5579-5584 NNP denotes Sigma
T5046 5584-5585 -RRB- denotes )
T5047 5586-5590 VB denotes were
T5048 5591-5596 VB denotes added
T5049 5596-5597 -COMMA- denotes ,
T5050 5598-5601 DT denotes the
T5051 5602-5610 NN denotes solution
T5052 5611-5614 VB denotes was
T5053 5615-5621 RB denotes gently
T5054 5622-5627 VB denotes mixed
T5055 5627-5628 -COMMA- denotes ,
T5056 5629-5638 VB denotes overlayed
T5057 5639-5643 IN denotes with
T5058 5644-5651 NN denotes mineral
T5059 5652-5655 NN denotes oil
T5060 5656-5659 CC denotes and
T5061 5660-5669 VB denotes incubated
T5062 5670-5672 IN denotes in
T5063 5673-5676 DT denotes the
T5064 5677-5681 NN denotes dark
T5065 5682-5685 IN denotes for
T5066 5686-5691 CD denotes 12–13
T5067 5692-5693 NN denotes h
T5068 5694-5696 IN denotes at
T5069 5697-5702 NNP denotes 50°C.
T5070 5703-5706 DT denotes The
T5071 5707-5714 JJ denotes aqueous
T5072 5715-5720 NN denotes phase
T5073 5721-5724 VB denotes was
T5074 5725-5734 VB denotes recovered
T5075 5735-5740 VB denotes using
T5076 5741-5744 DT denotes the
T5077 5745-5752 NNP denotes ‘Wizard
T5078 5753-5756 NN denotes DNA
T5079 5757-5765 NN denotes clean-up
T5080 5766-5773 NN denotes system’
T5081 5774-5775 -LRB- denotes (
T5082 5775-5782 NNP denotes Promega
T5083 5782-5783 -COMMA- denotes ,
T5084 5784-5792 NNP denotes Mannheim
T5085 5792-5793 -COMMA- denotes ,
T5086 5794-5801 NNP denotes Germany
T5087 5801-5802 -RRB- denotes )
T5088 5804-5807 DT denotes The
T5089 5808-5816 VB denotes purified
T5090 5817-5820 NN denotes DNA
T5091 5821-5824 VB denotes was
T5092 5825-5837 RB denotes subsequently
T5093 5838-5843 VB denotes mixed
T5094 5844-5848 IN denotes with
T5095 5849-5850 CD denotes 1
T5096 5851-5852 NN denotes M
T5097 5853-5857 NN denotes NaOH
T5098 5858-5860 TO denotes to
T5099 5861-5862 DT denotes a
T5100 5863-5868 JJ denotes final
T5101 5869-5882 NN denotes concentration
T5102 5883-5885 IN denotes of
T5103 5886-5889 CD denotes 0.3
T5104 5890-5891 NN denotes M
T5105 5892-5895 CC denotes and
T5106 5896-5905 VB denotes incubated
T5107 5906-5909 IN denotes for
T5108 5910-5912 CD denotes 20
T5109 5913-5916 NN denotes min
T5110 5917-5919 IN denotes at
T5111 5920-5924 NN denotes 37°C
T5112 5925-5927 TO denotes to
T5113 5928-5934 VB denotes ensure
T5114 5935-5943 JJ denotes complete
T5115 5944-5959 NN denotes desulfonisation
T5116 5961-5964 NN denotes DNA
T5117 5965-5968 VB denotes was
T5118 5969-5976 NN denotes ethanol
T5119 5977-5989 VB denotes precipitated
T5120 5990-5992 IN denotes in
T5121 5993-5996 DT denotes the
T5122 5997-6005 NN denotes presence
T5123 6006-6008 IN denotes of
T5124 6009-6013 CD denotes 1/10
T5125 6014-6017 NN denotes vol
T5126 6018-6020 IN denotes of
T5127 6021-6022 CD denotes 3
T5128 6023-6024 NN denotes M
T5129 6025-6031 NN denotes sodium
T5130 6032-6039 NN denotes acetate
T5131 6039-6040 -COMMA- denotes ,
T5132 6041-6047 VB denotes washed
T5133 6048-6052 IN denotes with
T5134 6053-6055 CD denotes 70
T5135 6055-6056 NN denotes %
T5136 6057-6064 NN denotes ethanol
T5137 6065-6068 CC denotes and
T5138 6069-6080 VB denotes resuspended
T5139 6081-6083 IN denotes in
T5140 6084-6086 CD denotes 50
T5141 6087-6089 NN denotes µl
T5142 6090-6093 NN denotes H2O
T5143 6095-6105 JJ denotes Subsequent
T5144 6106-6109 NN denotes PCR
T5145 6110-6123 NN denotes amplification
T5146 6124-6126 IN denotes of
T5147 6127-6128 CD denotes 4
T5148 6129-6131 NN denotes µl
T5149 6132-6149 JJ denotes bisulfite-treated
T5150 6150-6153 NN denotes DNA
T5151 6154-6157 VB denotes was
T5152 6158-6162 VB denotes used
T5153 6163-6166 IN denotes for
T5154 6167-6174 NN denotes cloning
T5155 6175-6177 IN denotes of
T5156 6178-6181 CD denotes two
T5157 6182-6191 NN denotes fragments
T5158 6192-6194 IN denotes of
T5159 6195-6198 DT denotes the
T5160 6199-6204 NN denotes IRF-4
T5161 6205-6213 NN denotes promoter
T5162 6214-6215 -LRB- denotes (
T5163 6215-6219 NN denotes BS-I
T5164 6220-6223 CC denotes and
T5165 6224-6229 NN denotes BS-II
T5166 6229-6230 -RRB- denotes )
T5167 6231-6235 IN denotes into
T5168 6236-6242 NN denotes pCR2.1
T5169 6243-6249 NN denotes vector
T5170 6250-6254 IN denotes with
T5171 6255-6258 DT denotes the
T5172 6259-6264 NN denotes ‘TOPO
T5173 6265-6267 NN denotes TA
T5174 6268-6275 NN denotes cloning
T5175 6276-6280 NN denotes kit’
T5176 6281-6282 -LRB- denotes (
T5177 6282-6292 NN denotes Invitrogen
T5178 6292-6293 -RRB- denotes )
T5179 6294-6295 -LRB- denotes (
T5180 6295-6298 VB denotes see
T5181 6299-6305 NN denotes Figure
T5182 6306-6308 NN denotes 3A
T5183 6308-6309 -RRB- denotes )
T5184 6311-6314 DT denotes The
T5185 6315-6322 NN denotes primers
T5186 6323-6327 VB denotes used
T5187 6328-6331 IN denotes for
T5188 6332-6335 NN denotes PCR
T5189 6336-6349 NN denotes amplification
T5190 6350-6352 IN denotes of
T5191 6353-6356 DT denotes the
T5192 6357-6361 NN denotes BS-I
T5193 6362-6365 CC denotes and
T5194 6366-6371 NN denotes BS-II
T5195 6372-6381 NN denotes fragments
T5196 6382-6389 VB denotes contain
T5197 6390-6393 DT denotes the
T5198 6394-6402 JJ denotes putative
T5199 6403-6410 JJ denotes altered
T5200 6411-6419 NN denotes sequence
T5201 6420-6422 IN denotes of
T5202 6423-6426 DT denotes the
T5203 6427-6432 NN denotes sense
T5204 6433-6439 NN denotes strand
T5205 6440-6443 JJ denotes due
T5206 6444-6446 TO denotes to
T5207 6447-6456 VB denotes bisulfite
T5208 6457-6466 NN denotes treatment
T5209 6467-6468 -LRB- denotes (
T5210 6468-6477 VB denotes converted
T5211 6478-6486 NN denotes cytosine
T5212 6487-6495 NN denotes residues
T5213 6496-6499 VB denotes are
T5214 6500-6507 VB denotes written
T5215 6508-6510 IN denotes in
T5216 6511-6515 JJ denotes bold
T5217 6516-6523 NN denotes letters
T5218 6523-6524 -RRB- denotes )
T5219 6524-6525 -COLON- denotes :
T5220 6526-6538 JJ denotes BS-I-forward
T5221 6539-6572 NN denotes 5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′
T5222 6572-6573 -COMMA- denotes ,
T5223 6574-6586 JJ denotes BS-I-reverse
T5224 6587-6619 NN denotes 5′-ACCCAACTCCCTTAAACTATTAAACT-3′
T5225 6620-6621 -LRB- denotes (
T5226 6621-6624 CD denotes 187
T5227 6625-6627 NN denotes bp
T5228 6627-6628 -RRB- denotes )
T5229 6628-6629 -COLON- denotes ;
T5230 6630-6643 JJ denotes BS-II-forward
T5231 6644-6676 NN denotes 5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′
T5232 6676-6677 -COMMA- denotes ,
T5233 6678-6691 JJ denotes BS-II-reverse
T5234 6692-6724 NN denotes 5′-CTCACCCTAAACTCAAAACTAAAAAC-3′
T5235 6725-6726 -LRB- denotes (
T5236 6726-6729 CD denotes 674
T5237 6730-6732 NN denotes bp
T5238 6732-6733 -RRB- denotes )
T5239 6735-6740 IN denotes After
T5240 6741-6750 JJ denotes bacterial
T5241 6751-6764 NN denotes amplification
T5242 6765-6767 IN denotes of
T5243 6768-6771 DT denotes the
T5244 6772-6778 VB denotes cloned
T5245 6779-6782 NN denotes PCR
T5246 6783-6792 NN denotes fragments
T5247 6793-6795 IN denotes by
T5248 6796-6804 JJ denotes standard
T5249 6805-6815 NN denotes procedures
T5250 6815-6816 -COMMA- denotes ,
T5251 6817-6822 CD denotes eight
T5252 6823-6829 NN denotes clones
T5253 6830-6834 IN denotes from
T5254 6835-6839 DT denotes each
T5255 6840-6846 NN denotes sample
T5256 6847-6851 VB denotes were
T5257 6852-6861 VB denotes sequenced
T5258 6862-6866 IN denotes with
T5259 6867-6869 DT denotes an
T5260 6870-6879 VB denotes automated
T5261 6880-6889 NN denotes sequencer
T5262 6890-6891 -LRB- denotes (
T5263 6891-6894 NNP denotes ABI
T5264 6895-6900 NNP denotes Prism
T5265 6901-6904 CD denotes 377
T5266 6904-6905 -COMMA- denotes ,
T5267 6906-6913 NNP denotes Applied
T5268 6914-6924 NNP denotes Biosystems
T5269 6924-6925 -RRB- denotes )
T5701 6928-6930 FW denotes In
T5702 6931-6936 FW denotes vitro
T5703 6937-6948 NN denotes methylation
T5704 6949-6952 CC denotes and
T5705 6953-6961 NN denotes reporter
T5706 6962-6966 NN denotes gene
T5707 6967-6973 NN denotes assays
T5708 6974-6977 DT denotes The
T5709 6978-6983 NN denotes IRF-4
T5710 6984-7001 JJ denotes promoter-reporter
T5711 7002-7006 NN denotes gene
T5712 7007-7016 NN denotes construct
T5713 7017-7020 VB denotes was
T5714 7021-7031 RB denotes generously
T5715 7032-7040 VB denotes provided
T5716 7041-7043 IN denotes by
T5717 7044-7046 NNP denotes J.
T5718 7047-7054 NNP denotes Hiscott
T5719 7055-7056 -LRB- denotes (
T5720 7056-7058 CD denotes 31
T5721 7058-7059 -RRB- denotes )
T5722 7061-7071 NN denotes Constructs
T5723 7072-7076 VB denotes were
T5724 7077-7087 VB denotes methylated
T5725 7088-7090 FW denotes in
T5726 7091-7096 FW denotes vitro
T5727 7097-7101 IN denotes with
T5728 7102-7105 NN denotes CpG
T5729 7106-7115 NN denotes Methylase
T5730 7116-7117 -LRB- denotes (
T5731 7117-7122 NN denotes M.Sss
T5732 7123-7124 CD denotes I
T5733 7124-7125 -RRB- denotes )
T5734 7126-7128 IN denotes as
T5735 7129-7140 VB denotes recommended
T5736 7141-7143 IN denotes by
T5737 7144-7147 DT denotes the
T5738 7148-7160 NN denotes manufacturer
T5739 7161-7162 -LRB- denotes (
T5740 7162-7164 NN denotes NE
T5741 7165-7172 NN denotes Biolabs
T5742 7172-7173 -RRB- denotes )
T5743 7174-7177 CC denotes and
T5744 7178-7186 JJ denotes complete
T5745 7187-7198 NN denotes methylation
T5746 7199-7202 VB denotes was
T5747 7203-7210 VB denotes checked
T5748 7211-7214 IN denotes via
T5749 7215-7226 NN denotes restriction
T5750 7227-7235 NN denotes analysis
T5751 7236-7237 -LRB- denotes (
T5752 7237-7243 NN denotes Figure
T5753 7244-7246 NN denotes 5A
T5754 7246-7247 -RRB- denotes )
T5755 7249-7257 NN denotes Reporter
T5756 7258-7262 NN denotes gene
T5757 7263-7269 NN denotes assays
T5758 7270-7275 VB denotes using
T5759 7276-7279 DT denotes the
T5760 7280-7284 JJ denotes dual
T5761 7285-7295 NN denotes luciferase
T5762 7296-7301 NN denotes assay
T5763 7302-7303 -LRB- denotes (
T5764 7303-7310 NN denotes Promega
T5765 7310-7311 -RRB- denotes )
T5766 7312-7316 VB denotes were
T5767 7317-7326 VB denotes performed
T5768 7327-7334 JJ denotes similar
T5769 7335-7337 TO denotes to
T5770 7338-7346 JJ denotes previous
T5771 7347-7354 NN denotes reports
T5772 7355-7356 -LRB- denotes (
T5773 7356-7358 CD denotes 29
T5774 7358-7359 -RRB- denotes )
T5775 7361-7368 RB denotes Briefly
T5776 7368-7369 -COMMA- denotes ,
T5777 7370-7371 CD denotes 5
T5778 7372-7374 NN denotes nM
T5779 7375-7377 IN denotes of
T5780 7378-7381 DT denotes the
T5781 7382-7390 NN denotes reporter
T5782 7391-7400 NN denotes construct
T5783 7401-7404 CC denotes and
T5784 7405-7408 DT denotes the
T5785 7409-7421 NN denotes transfection
T5786 7422-7429 NN denotes control
T5787 7430-7439 NN denotes construct
T5788 7440-7450 VB denotes expressing
T5789 7451-7454 DT denotes the
T5790 7455-7462 NN denotes renilla
T5791 7463-7473 NN denotes luciferase
T5792 7474-7478 NN denotes gene
T5793 7479-7483 VB denotes were
T5794 7484-7507 VB denotes transientlyco-expressed
T5795 7508-7511 IN denotes via
T5796 7512-7527 NN denotes electroporation
T5797 7529-7532 DT denotes The
T5798 7533-7540 NN denotes control
T5799 7541-7550 NN denotes construct
T5800 7551-7557 VB denotes served
T5801 7558-7560 IN denotes as
T5802 7561-7563 DT denotes an
T5803 7564-7572 JJ denotes internal
T5804 7573-7582 NN denotes reference
T5805 7583-7586 IN denotes for
T5806 7587-7599 NN denotes transfection
T5807 7600-7610 NN denotes efficiency
T5808 7612-7623 CD denotes Forty-eight
T5809 7624-7629 NN denotes hours
T5810 7630-7635 IN denotes after
T5811 7636-7648 NN denotes transfection
T5812 7648-7649 -COMMA- denotes ,
T5813 7650-7660 NN denotes luciferase
T5814 7661-7669 NN denotes activity
T5815 7670-7673 VB denotes was
T5816 7674-7682 VB denotes measured
T5817 7683-7687 IN denotes with
T5818 7688-7689 DT denotes a
T5819 7690-7692 NN denotes LB
T5820 7693-7695 CD denotes 96
T5821 7696-7697 NN denotes P
T5822 7698-7708 NN denotes microlumat
T5823 7709-7710 -LRB- denotes (
T5824 7710-7712 NNP denotes EG
T5825 7712-7713 CC denotes &
T5826 7713-7714 NNP denotes G
T5827 7715-7723 NNP denotes Berthold
T5828 7723-7724 -COMMA- denotes ,
T5829 7725-7728 NNP denotes Bad
T5830 7729-7736 NNP denotes Wildbad
T5831 7736-7737 -COMMA- denotes ,
T5832 7738-7745 NNP denotes Germany
T5833 7745-7746 -RRB- denotes )
T5834 7748-7753 NN denotes IRF-4
T5835 7754-7762 NN denotes promoter
T5836 7763-7773 NN denotes activation
T5837 7774-7777 VB denotes was
T5838 7778-7788 VB denotes quantified
T5839 7789-7791 IN denotes as
T5840 7792-7793 DT denotes a
T5841 7794-7799 NN denotes ratio
T5842 7800-7802 IN denotes of
T5843 7803-7811 VB denotes measured
T5844 7812-7819 RB denotes firefly
T5845 7820-7825 JJ denotes light
T5846 7826-7831 NN denotes units
T5847 7832-7833 -LRB- denotes (
T5848 7833-7836 NN denotes flu
T5849 7836-7837 -RRB- denotes )
T5850 7838-7846 JJ denotes relative
T5851 7847-7849 TO denotes to
T5852 7850-7857 NN denotes renilla
T5853 7858-7859 -LRB- denotes (
T5854 7859-7862 NN denotes rlu
T5855 7862-7863 -RRB- denotes )
T5856 7865-7869 DT denotes Each
T5857 7870-7880 NN denotes experiment
T5858 7881-7884 VB denotes was
T5859 7885-7892 VB denotes carried
T5860 7893-7896 RP denotes out
T5861 7897-7899 IN denotes at
T5862 7900-7905 JJ denotes least
T5863 7906-7911 CD denotes three
T5864 7912-7917 NN denotes times
R2131 T2711 T2710 arg1Of lines,Cell
R2132 T2712 T2713 arg1Of K-562,","
R2133 T2713 T2715 arg1Of ",",and
R2134 T2714 T2713 arg2Of Jurkat,","
R2135 T2715 T2717 arg1Of and,were
R2136 T2715 T2718 arg2Of and,obtained
R2137 T2716 T2715 arg2Of U-937,and
R2138 T2718 T2717 arg2Of obtained,were
R2139 T2718 T2719 arg1Of obtained,from
R2140 T2718 T2742 arg1Of obtained,and
R2141 T2721 T2722 arg1Of ATCC,(
R2142 T2721 T2732 arg1Of ATCC,and
R2143 T2726 T2722 arg2Of Collection,(
R2144 T2726 T2723 arg1Of Collection,American
R2145 T2726 T2724 arg1Of Collection,Type
R2146 T2726 T2725 arg1Of Collection,Culture
R2147 T2726 T2727 arg1Of Collection,","
R2148 T2728 T2727 arg2Of Rockville,","
R2149 T2728 T2729 arg1Of Rockville,","
R2150 T2730 T2729 arg2Of USA,","
R2151 T2731 T2722 arg3Of ),(
R2152 T2732 T2719 arg2Of and,from
R2153 T2732 T2720 arg1Of and,the
R2154 T2733 T2734 arg1Of EM-2,","
R2155 T2734 T2736 arg1Of ",",","
R2156 T2735 T2734 arg2Of LAMA-84,","
R2157 T2736 T2738 arg1Of ",",","
R2158 T2737 T2736 arg2Of CML-T1,","
R2159 T2738 T2740 arg1Of ",",","
R2160 T2739 T2738 arg2Of BV-173,","
R2161 T2740 T2732 arg2Of ",",and
R2162 T2741 T2740 arg2Of SD-1,","
R2163 T2743 T2744 arg1Of RPMI-8226,from
R2164 T2743 T2752 arg1Of RPMI-8226,und
R2165 T2751 T2744 arg2Of Mikroorganismen,from
R2166 T2751 T2745 arg1Of Mikroorganismen,the
R2167 T2751 T2746 arg1Of Mikroorganismen,DSMZ
R2168 T2751 T2747 arg1Of Mikroorganismen,(
R2169 T2751 T2748 arg1Of Mikroorganismen,Deutsche
R2170 T2751 T2749 arg1Of Mikroorganismen,Sammlung
R2171 T2751 T2750 arg1Of Mikroorganismen,von
R2172 T2752 T2742 arg2Of und,and
R2173 T2754 T2753 arg1Of GmbH,Zellkulturen
R2174 T2754 T2755 arg1Of GmbH,","
R2175 T2755 T2752 arg2Of ",",und
R2176 T2755 T2757 arg1Of ",",","
R2177 T2756 T2755 arg2Of Braunschweig,","
R2178 T2758 T2757 arg2Of Germany,","
R2179 T2758 T2759 arg1Of Germany,)
R2180 T2762 T2760 arg1Of lines,All
R2181 T2762 T2761 arg1Of lines,cell
R2182 T2762 T2771 arg1Of lines,were
R2183 T2762 T2772 arg1Of lines,IRF-4-negative
R2184 T2765 T2766 arg1Of BV-173,","
R2185 T2766 T2768 arg1Of ",",and
R2186 T2767 T2766 arg2Of SD-1,","
R2187 T2768 T2764 arg2Of and,except
R2188 T2769 T2768 arg2Of RPMI-8226,and
R2189 T2771 T2763 arg1Of were,","
R2190 T2771 T2764 arg1Of were,except
R2191 T2771 T2770 arg1Of were,","
R2192 T2772 T2771 arg2Of IRF-4-negative,were
R2263 T2863 T2864 arg1Of culture,and
R2264 T2864 T2862 arg1Of and,Cell
R2265 T2865 T2864 arg2Of stimulation,and
R2266 T2868 T2866 arg1Of lines,All
R2267 T2868 T2867 arg1Of lines,cell
R2268 T2868 T2869 arg1Of lines,were
R2269 T2868 T2870 arg2Of lines,maintained
R2270 T2870 T2869 arg2Of maintained,were
R2271 T2870 T2871 arg1Of maintained,at
R2272 T2872 T2873 arg1Of 5,%
R2273 T2874 T2871 arg2Of CO2,at
R2274 T2874 T2872 arg1Of CO2,5
R2275 T2874 T2875 arg1Of CO2,in
R2276 T2874 T2879 arg1Of CO2,with
R2277 T2874 T2889 arg2Of CO2,supplemented
R2278 T2878 T2875 arg2Of medium,in
R2279 T2878 T2876 arg1Of medium,RPMI
R2280 T2878 T2877 arg1Of medium,1640
R2281 T2880 T2881 arg1Of 1,%
R2282 T2882 T2879 arg2Of glutamine,with
R2283 T2882 T2880 arg1Of glutamine,1
R2284 T2882 T2883 arg1Of glutamine,(
R2285 T2885 T2883 arg2Of Eggenstein,(
R2286 T2885 T2884 arg1Of Eggenstein,Gibco/BRL
R2287 T2885 T2886 arg1Of Eggenstein,","
R2288 T2887 T2886 arg2Of Germany,","
R2289 T2888 T2883 arg3Of ),(
R2290 T2889 T2890 arg1Of supplemented,with
R2291 T2889 T2903 arg1Of supplemented,(
R2292 T2891 T2892 arg1Of 10,%
R2293 T2895 T2891 arg1Of serum,10
R2294 T2895 T2893 arg1Of serum,fetal
R2295 T2895 T2894 arg1Of serum,calf
R2296 T2895 T2896 arg1Of serum,(
R2297 T2895 T2899 arg1Of serum,","
R2298 T2897 T2896 arg2Of Gibco/BRL,(
R2299 T2898 T2896 arg3Of ),(
R2300 T2899 T2890 arg2Of ",",with
R2301 T2900 T2901 arg1Of 1,%
R2302 T2902 T2899 arg2Of penicillin/streptomycin,","
R2303 T2902 T2900 arg1Of penicillin/streptomycin,1
R2304 T2904 T2905 arg1Of Biochrom,","
R2305 T2905 T2903 arg2Of ",",(
R2306 T2905 T2907 arg1Of ",",","
R2307 T2906 T2905 arg2Of Berlin,","
R2308 T2908 T2907 arg2Of Germany,","
R2309 T2909 T2903 arg3Of ),(
R2310 T2913 T2911 arg2Of cells,indicated
R2311 T2913 T2914 arg1Of cells,were
R2312 T2913 T2915 arg2Of cells,treated
R2313 T2915 T2910 arg1Of treated,When
R2314 T2915 T2911 modOf treated,indicated
R2315 T2915 T2912 arg1Of treated,","
R2316 T2915 T2914 arg2Of treated,were
R2317 T2915 T2916 arg1Of treated,with
R2318 T2915 T2926 arg1Of treated,(
R2319 T2915 T2933 arg1Of treated,for
R2320 T2917 T2918 arg1Of 5-aza-2-deoxycytidine,(
R2321 T2917 T2921 arg1Of 5-aza-2-deoxycytidine,or
R2322 T2919 T2918 arg2Of AzadC,(
R2323 T2920 T2918 arg3Of ),(
R2324 T2921 T2916 arg2Of or,with
R2325 T2922 T2921 arg2Of 5-azacytidine,or
R2326 T2922 T2923 arg1Of 5-azacytidine,(
R2327 T2924 T2923 arg2Of AzaC,(
R2328 T2925 T2923 arg3Of ),(
R2329 T2927 T2928 arg1Of Sigma,","
R2330 T2928 T2926 arg2Of ",",(
R2331 T2928 T2930 arg1Of ",",","
R2332 T2929 T2928 arg2Of Taufkirchen,","
R2333 T2931 T2930 arg2Of Germany,","
R2334 T2932 T2926 arg3Of ),(
R2335 T2936 T2933 arg2Of periods,for
R2336 T2936 T2934 arg1Of periods,different
R2337 T2936 T2935 arg1Of periods,time
R2338 T2937 T2938 arg1Of Owing,to
R2339 T2937 T2945 arg1Of Owing,re-added
R2340 T2940 T2938 arg2Of chemical,to
R2341 T2940 T2939 arg1Of chemical,their
R2342 T2941 T2937 arg2Of instability,Owing
R2343 T2941 T2944 arg2Of instability,were
R2344 T2943 T2942 arg1Of substances,fresh
R2345 T2943 T2944 arg1Of substances,were
R2346 T2948 T2945 arg2Of h,re-added
R2347 T2948 T2946 arg1Of h,every
R2348 T2948 T2947 arg1Of h,24
R2438 T3062 T3063 arg1Of Sequencing,of
R2439 T3066 T3063 arg2Of promoter,of
R2440 T3066 T3064 arg1Of promoter,the
R2441 T3066 T3065 arg1Of promoter,IRF-4
R2442 T3068 T3067 arg2Of analysis,For
R2443 T3068 T3069 arg1Of analysis,of
R2444 T3073 T3069 arg2Of region,of
R2445 T3073 T3070 arg1Of region,the
R2446 T3073 T3071 arg1Of region,IRF-4
R2447 T3073 T3072 arg1Of region,promoter
R2448 T3073 T3074 arg1Of region,for
R2449 T3076 T3074 arg2Of aberrations,for
R2450 T3076 T3075 arg1Of aberrations,permanent
R2451 T3076 T3078 arg1Of aberrations,as
R2452 T3078 T3077 arg1Of as,such
R2453 T3079 T3080 arg1Of insertions/deletions,or
R2454 T3080 T3078 arg2Of or,as
R2455 T3081 T3080 arg2Of mutation,or
R2456 T3083 T3084 arg1Of we,PCR-amplified
R2457 T3084 T3067 arg1Of PCR-amplified,For
R2458 T3084 T3082 arg1Of PCR-amplified,","
R2459 T3086 T3084 arg2Of fragments,PCR-amplified
R2460 T3086 T3085 arg1Of fragments,two
R2461 T3086 T3087 arg1Of fragments,from
R2462 T3089 T3087 arg2Of DNA,from
R2463 T3089 T3088 arg1Of DNA,genomic
R2464 T3089 T3090 arg1Of DNA,","
R2465 T3089 T3091 arg1Of DNA,which
R2466 T3089 T3092 arg1Of DNA,was
R2467 T3089 T3093 arg2Of DNA,extracted
R2468 T3093 T3092 arg2Of extracted,was
R2469 T3093 T3094 arg1Of extracted,from
R2470 T3093 T3109 arg1Of extracted,as
R2471 T3097 T3094 arg2Of lines,from
R2472 T3097 T3095 arg2Of lines,depicted
R2473 T3097 T3096 arg1Of lines,cell
R2474 T3097 T3098 arg1Of lines,with
R2475 T3097 T3102 arg1Of lines,(
R2476 T3101 T3098 arg2Of kit,with
R2477 T3101 T3099 arg1Of kit,a
R2478 T3101 T3100 arg1Of kit,commercial
R2479 T3103 T3102 arg2Of Qiagen,(
R2480 T3103 T3104 arg1Of Qiagen,","
R2481 T3103 T3106 arg1Of Qiagen,","
R2482 T3105 T3104 arg2Of Hilde,","
R2483 T3107 T3106 arg2Of Germany,","
R2484 T3108 T3102 arg3Of ),(
R2485 T3110 T3109 arg2Of recommended,as
R2486 T3112 T3111 arg1Of primers,The
R2487 T3112 T3113 arg1Of primers,were
R2488 T3112 T3114 arg1Of primers,1-forward
R2489 T3113 T3115 arg1Of were,:
R2490 T3114 T3113 arg2Of 1-forward,were
R2491 T3116 T3117 arg1Of 5′-TTGAGATGGAGTCTTGCTCTGT-3′,","
R2492 T3117 T3119 arg1Of ",",:
R2493 T3118 T3117 arg2Of 1-reverse,","
R2494 T3120 T3121 arg1Of 5′-CCAGGACCTCAGGAGGCCAGTCA-3′,;
R2495 T3121 T3123 arg1Of ;,:
R2496 T3122 T3121 arg2Of 2-forward,;
R2497 T3124 T3125 arg1Of 5′-AGCGGTGAAACTGAGAGTGCGAGGT-3′,","
R2498 T3125 T3127 arg1Of ",",:
R2499 T3126 T3125 arg2Of 2-reverse,","
R2500 T3130 T3129 arg1Of products,The
R2501 T3130 T3131 arg1Of products,were
R2502 T3130 T3132 arg2Of products,cloned
R2503 T3132 T3131 arg2Of cloned,were
R2504 T3132 T3133 arg1Of cloned,with
R2505 T3138 T3133 arg2Of kit’,with
R2506 T3138 T3134 arg1Of kit’,the
R2507 T3138 T3135 arg1Of kit’,‘TOPO
R2508 T3138 T3136 arg1Of kit’,TA
R2509 T3138 T3137 arg1Of kit’,cloning
R2510 T3138 T3139 arg1Of kit’,(
R2511 T3140 T3139 arg2Of Invitrogen,(
R2512 T3140 T3141 arg1Of Invitrogen,","
R2513 T3140 T3143 arg1Of Invitrogen,","
R2514 T3142 T3141 arg2Of Groningen,","
R2515 T3145 T3143 arg2Of Netherlands,","
R2516 T3145 T3144 arg1Of Netherlands,The
R2517 T3146 T3139 arg3Of ),(
R2518 T3149 T3147 arg2Of amplification,After
R2519 T3149 T3148 arg1Of amplification,bacterial
R2520 T3149 T3150 arg1Of amplification,of
R2521 T3149 T3155 arg1Of amplification,by
R2522 T3154 T3150 arg2Of fragments,of
R2523 T3154 T3151 arg1Of fragments,the
R2524 T3154 T3152 arg2Of fragments,cloned
R2525 T3154 T3153 arg1Of fragments,PCR
R2526 T3157 T3155 arg2Of procedures,by
R2527 T3157 T3156 arg1Of procedures,standard
R2528 T3161 T3159 arg1Of three,at
R2529 T3161 T3160 arg1Of three,least
R2530 T3162 T3161 arg1Of clones,three
R2531 T3162 T3163 arg1Of clones,from
R2532 T3162 T3166 arg1Of clones,were
R2533 T3162 T3167 arg2Of clones,sequenced
R2534 T3165 T3163 arg2Of sample,from
R2535 T3165 T3164 arg1Of sample,each
R2536 T3167 T3147 arg1Of sequenced,After
R2537 T3167 T3158 arg1Of sequenced,","
R2538 T3167 T3166 arg2Of sequenced,were
R2539 T3167 T3168 arg1Of sequenced,with
R2540 T3167 T3172 arg1Of sequenced,(
R2541 T3167 T3185 arg1Of sequenced,as
R2542 T3171 T3168 arg2Of sequencer,with
R2543 T3171 T3169 arg1Of sequencer,an
R2544 T3171 T3170 arg2Of sequencer,automated
R2545 T3174 T3173 arg1Of Prism,ABI
R2546 T3174 T3175 arg1Of Prism,377
R2547 T3174 T3176 arg1Of Prism,","
R2548 T3174 T3179 arg1Of Prism,","
R2549 T3178 T3176 arg2Of Bio-systems,","
R2550 T3178 T3177 arg1Of Bio-systems,Applied
R2551 T3179 T3172 arg2Of ",",(
R2552 T3179 T3182 arg1Of ",",","
R2553 T3179 T3183 arg1Of ",",USA
R2554 T3181 T3179 arg2Of City,","
R2555 T3181 T3180 arg1Of City,Foster
R2556 T3184 T3172 arg3Of ),(
R2557 T3186 T3185 arg2Of recommended,as
R2558 T3189 T3186 arg1Of manufacturer,recommended
R2559 T3189 T3187 arg2Of manufacturer,by
R2560 T3189 T3188 arg1Of manufacturer,the
R2749 T3451 T3450 arg1Of analysis,Expression
R2750 T3453 T3452 arg1Of analyze,To
R2751 T3457 T3453 arg2Of level,analyze
R2752 T3457 T3454 arg1Of level,the
R2753 T3457 T3455 arg1Of level,IRF-4
R2754 T3457 T3456 arg1Of level,transcriptional
R2755 T3459 T3453 arg1Of RNA,analyze
R2756 T3459 T3460 arg1Of RNA,was
R2757 T3459 T3461 arg2Of RNA,extracted
R2758 T3461 T3452 modOf extracted,To
R2759 T3461 T3458 arg1Of extracted,","
R2760 T3461 T3460 arg2Of extracted,was
R2761 T3461 T3462 arg1Of extracted,from
R2762 T3463 T3462 arg2Of cells,from
R2763 T3463 T3464 arg1Of cells,using
R2764 T3467 T3464 arg2Of RNAzol-kit,using
R2765 T3467 T3465 arg1Of RNAzol-kit,the
R2766 T3467 T3466 arg1Of RNAzol-kit,commercial
R2767 T3467 T3468 arg1Of RNAzol-kit,(
R2768 T3469 T3470 arg1Of Paesel,","
R2769 T3470 T3468 arg2Of ",",(
R2770 T3470 T3472 arg1Of ",",","
R2771 T3471 T3470 arg2Of Frankfurt,","
R2772 T3473 T3472 arg2Of Germany,","
R2773 T3474 T3468 arg3Of ),(
R2774 T3476 T3475 arg1Of aliquot,An
R2775 T3476 T3477 arg1Of aliquot,of
R2776 T3476 T3482 arg1Of aliquot,was
R2777 T3476 T3483 arg2Of aliquot,used
R2778 T3481 T3477 arg2Of RNA,of
R2779 T3481 T3478 arg1Of RNA,1
R2780 T3481 T3479 arg1Of RNA,μg
R2781 T3481 T3480 arg1Of RNA,total
R2782 T3483 T3482 arg2Of used,was
R2783 T3483 T3484 arg1Of used,for
R2784 T3486 T3484 arg2Of synthesis,for
R2785 T3486 T3485 arg1Of synthesis,cDNA
R2786 T3487 T3483 arg3Of as,used
R2787 T3488 T3487 arg2Of described,as
R2788 T3488 T3489 arg1Of described,previously
R2789 T3489 T3490 arg1Of previously,(
R2790 T3491 T3490 arg2Of 27,(
R2791 T3492 T3490 arg3Of ),(
R2792 T3495 T3493 arg1Of analysis,RNA
R2793 T3495 T3494 arg1Of analysis,expression
R2794 T3495 T3496 arg1Of analysis,for
R2795 T3495 T3498 arg1Of analysis,and
R2796 T3497 T3496 arg2Of IRF-4,for
R2797 T3498 T3503 arg1Of and,was
R2798 T3498 T3504 arg2Of and,carried
R2799 T3502 T3498 arg2Of β-actin,and
R2800 T3502 T3499 arg1Of β-actin,the
R2801 T3502 T3500 arg1Of β-actin,reference
R2802 T3502 T3501 arg1Of β-actin,gene
R2803 T3504 T3503 arg2Of carried,was
R2804 T3504 T3505 arg1Of carried,out
R2805 T3504 T3509 arg1Of carried,as
R2806 T3508 T3504 arg1Of PCR,carried
R2807 T3508 T3506 arg2Of PCR,by
R2808 T3508 T3507 arg1Of PCR,semi-quantitative
R2809 T3510 T3509 arg2Of described,as
R2810 T3510 T3511 arg1Of described,previously
R2811 T3511 T3512 arg1Of previously,(
R2812 T3513 T3512 arg2Of "3,27",(
R2813 T3514 T3512 arg3Of ),(
R2814 T3516 T3515 arg1Of products,PCR
R2815 T3516 T3517 arg1Of products,were
R2816 T3516 T3518 arg2Of products,verified
R2817 T3518 T3517 arg2Of verified,were
R2818 T3521 T3518 arg1Of sequencing,verified
R2819 T3521 T3519 arg2Of sequencing,by
R2820 T3521 T3520 arg2Of sequencing,automated
R2821 T3523 T3522 arg1Of primers,PCR
R2822 T3523 T3524 arg1Of primers,and
R2823 T3524 T3526 arg1Of and,for
R2824 T3524 T3547 arg1Of and,were
R2825 T3524 T3548 arg2Of and,published
R2826 T3525 T3524 arg2Of conditions,and
R2827 T3528 T3526 arg2Of analysis,for
R2828 T3528 T3527 arg1Of analysis,expression
R2829 T3528 T3529 arg1Of analysis,of
R2830 T3530 T3531 arg1Of DNMT,or
R2831 T3531 T3529 arg2Of or,of
R2832 T3531 T3533 arg1Of or,(
R2833 T3532 T3531 arg2Of MBP,or
R2834 T3535 T3534 arg1Of DNMT3A,DNMT1
R2835 T3535 T3536 arg1Of DNMT3A,","
R2836 T3536 T3538 arg1Of ",",","
R2837 T3537 T3536 arg2Of DNMT3B,","
R2838 T3538 T3540 arg1Of ",",","
R2839 T3539 T3538 arg2Of MeCP,","
R2840 T3540 T3542 arg1Of ",",","
R2841 T3541 T3540 arg2Of MBD1,","
R2842 T3542 T3544 arg1Of ",",and
R2843 T3543 T3542 arg2Of MBD2,","
R2844 T3544 T3533 arg2Of and,(
R2845 T3545 T3544 arg2Of MBD4,and
R2846 T3546 T3533 arg3Of ),(
R2847 T3548 T3547 arg2Of published,were
R2848 T3548 T3549 arg1Of published,elsewhere
R2849 T3549 T3550 arg1Of elsewhere,(
R2850 T3551 T3550 arg2Of 28,(
R2851 T3552 T3550 arg3Of ),(
R2852 T3554 T3553 arg2Of analysis,For
R2853 T3554 T3555 arg1Of analysis,of
R2854 T3558 T3555 arg2Of expression,of
R2855 T3558 T3556 arg1Of expression,IRF-4
R2856 T3558 T3557 arg1Of expression,protein
R2857 T3563 T3560 arg1Of assay,a
R2858 T3563 T3561 arg1Of assay,standard
R2859 T3563 T3562 arg1Of assay,immunoblotting
R2860 T3563 T3564 arg1Of assay,was
R2861 T3563 T3565 arg2Of assay,performed
R2862 T3565 T3553 arg1Of performed,For
R2863 T3565 T3559 arg1Of performed,","
R2864 T3565 T3564 arg2Of performed,was
R2865 T3565 T3566 arg1Of performed,as
R2866 T3567 T3566 arg2Of described,as
R2867 T3567 T3568 arg1Of described,previously
R2868 T3568 T3569 arg1Of previously,(
R2869 T3570 T3569 arg2Of 29,(
R2870 T3571 T3569 arg3Of ),(
R2871 T3575 T3574 arg1Of lysates,protein
R2872 T3575 T3576 arg1Of lysates,were
R2873 T3575 T3577 arg2Of lysates,generated
R2874 T3577 T3572 arg1Of generated,Briefly
R2875 T3577 T3573 arg1Of generated,","
R2876 T3577 T3576 arg2Of generated,were
R2877 T3577 T3578 arg1Of generated,by
R2878 T3579 T3578 arg2Of incubating,by
R2879 T3579 T3618 arg1Of incubating,for
R2880 T3583 T3579 arg2Of cells,incubating
R2881 T3583 T3580 arg1Of cells,1
R2882 T3583 T3581 arg1Of cells,×
R2883 T3583 T3582 arg1Of cells,106
R2884 T3583 T3584 arg1Of cells,in
R2885 T3583 T3589 arg1Of cells,(
R2886 T3588 T3584 arg2Of buffer,in
R2887 T3588 T3585 arg1Of buffer,100
R2888 T3588 T3586 arg1Of buffer,µl
R2889 T3588 T3587 arg1Of buffer,RIPA
R2890 T3590 T3591 arg1Of 1,%
R2891 T3592 T3590 arg1Of NP-40,1
R2892 T3592 T3593 arg1Of NP-40,","
R2893 T3593 T3597 arg1Of ",",","
R2894 T3594 T3595 arg1Of 0.5,%
R2895 T3596 T3593 arg2Of sodiumdesoxycholate,","
R2896 T3596 T3594 arg1Of sodiumdesoxycholate,0.5
R2897 T3597 T3601 arg1Of ",",","
R2898 T3598 T3599 arg1Of 0.1,%
R2899 T3600 T3597 arg2Of SDS,","
R2900 T3600 T3598 arg1Of SDS,0.1
R2901 T3601 T3606 arg1Of ",",","
R2902 T3605 T3601 arg2Of fluoride,","
R2903 T3605 T3602 arg1Of fluoride,100
R2904 T3605 T3603 arg1Of fluoride,µg/ml
R2905 T3605 T3604 arg1Of fluoride,phenylmethylsulfonyl
R2906 T3606 T3610 arg1Of ",",","
R2907 T3609 T3606 arg2Of protease-inhibitory-mix,","
R2908 T3609 T3607 arg1Of protease-inhibitory-mix,10
R2909 T3609 T3608 arg1Of protease-inhibitory-mix,µl/ml
R2910 T3610 T3589 arg2Of ",",(
R2911 T3613 T3610 arg2Of sodiumorthovanadate,","
R2912 T3613 T3611 arg1Of sodiumorthovanadate,1
R2913 T3613 T3612 arg1Of sodiumorthovanadate,µmol/ml
R2914 T3613 T3614 arg1Of sodiumorthovanadate,in
R2915 T3616 T3614 arg2Of saline,in
R2916 T3616 T3615 arg1Of saline,phosphate-buffered
R2917 T3617 T3589 arg3Of ),(
R2918 T3620 T3618 arg2Of min,for
R2919 T3620 T3619 arg1Of min,30
R2920 T3620 T3621 arg1Of min,on
R2921 T3622 T3621 arg2Of ice,on
R2922 T3624 T3623 arg2Of centrifugation,After
R2923 T3627 T3626 arg1Of concentration,protein
R2924 T3627 T3628 arg1Of concentration,of
R2925 T3627 T3631 arg1Of concentration,was
R2926 T3627 T3632 arg2Of concentration,determined
R2927 T3630 T3628 arg2Of supernatant,of
R2928 T3630 T3629 arg1Of supernatant,the
R2929 T3632 T3623 arg1Of determined,After
R2930 T3632 T3625 arg1Of determined,","
R2931 T3632 T3631 arg2Of determined,was
R2932 T3632 T3635 arg1Of determined,(
R2933 T3632 T3642 arg1Of determined,as
R2934 T3634 T3632 arg1Of BCA-method,determined
R2935 T3634 T3633 arg2Of BCA-method,by
R2936 T3636 T3637 arg1Of Pierce,","
R2937 T3637 T3635 arg2Of ",",(
R2938 T3637 T3639 arg1Of ",",","
R2939 T3638 T3637 arg2Of Rockford,","
R2940 T3640 T3639 arg2Of IL,","
R2941 T3641 T3635 arg3Of ),(
R2942 T3643 T3642 arg2Of recommended,as
R2943 T3645 T3644 arg1Of lysates,Protein
R2944 T3645 T3646 arg1Of lysates,(
R2945 T3645 T3650 arg1Of lysates,were
R2946 T3645 T3651 arg2Of lysates,electrophoresed
R2947 T3645 T3656 arg2Of lysates,transferred
R2948 T3648 T3646 arg2Of µg,(
R2949 T3648 T3647 arg1Of µg,70–100
R2950 T3649 T3646 arg3Of ),(
R2951 T3651 T3652 arg1Of electrophoresed,on
R2952 T3651 T3655 arg1Of electrophoresed,and
R2953 T3654 T3652 arg2Of gels,on
R2954 T3654 T3653 arg1Of gels,polyacrylamide
R2955 T3655 T3650 arg2Of and,were
R2956 T3656 T3655 arg2Of transferred,and
R2957 T3656 T3657 arg1Of transferred,to
R2958 T3659 T3657 arg2Of PVDF-membrane,to
R2959 T3659 T3658 arg1Of PVDF-membrane,a
R2960 T3659 T3660 arg1Of PVDF-membrane,(
R2961 T3662 T3660 arg2Of P,(
R2962 T3662 T3661 arg1Of P,Immobilon
R2963 T3662 T3663 arg1Of P,","
R2964 T3662 T3666 arg1Of P,;
R2965 T3665 T3663 arg2Of µm,","
R2966 T3665 T3664 arg1Of µm,0.45
R2967 T3667 T3666 arg2Of Millipore,;
R2968 T3667 T3668 arg1Of Millipore,","
R2969 T3669 T3668 arg2Of Eschborn,","
R2970 T3669 T3670 arg1Of Eschborn,","
R2971 T3671 T3670 arg2Of Germany,","
R2972 T3672 T3660 arg3Of ),(
R2973 T3673 T3674 arg1Of Membranes,were
R2974 T3673 T3675 arg2Of Membranes,blocked
R2975 T3673 T3715 arg2Of Membranes,incubated
R2976 T3675 T3676 arg1Of blocked,with
R2977 T3675 T3713 arg1Of blocked,and
R2978 T3678 T3676 arg2Of %,with
R2979 T3678 T3677 arg1Of %,2.5
R2980 T3678 T3679 arg1Of %,blocking
R2981 T3678 T3690 arg1Of %,(
R2982 T3680 T3679 arg2Of reagent,blocking
R2983 T3680 T3681 arg1Of reagent,(
R2984 T3680 T3687 arg1Of reagent,in
R2985 T3683 T3681 arg2Of Mannheim,(
R2986 T3683 T3682 arg1Of Mannheim,Boehringer
R2987 T3683 T3684 arg1Of Mannheim,","
R2988 T3685 T3684 arg2Of Germany,","
R2989 T3686 T3681 arg3Of ),(
R2990 T3689 T3687 arg2Of buffer,in
R2991 T3689 T3688 arg1Of buffer,TBST
R2992 T3693 T3691 arg1Of Tris–HCL,4.44
R2993 T3693 T3692 arg1Of Tris–HCL,g/l
R2994 T3693 T3694 arg1Of Tris–HCL,","
R2995 T3694 T3698 arg1Of ",",","
R2996 T3697 T3694 arg2Of TrisOH,","
R2997 T3697 T3695 arg1Of TrisOH,2.65
R2998 T3697 T3696 arg1Of TrisOH,g/l
R2999 T3698 T3702 arg1Of ",",","
R3000 T3701 T3698 arg2Of NaCl,","
R3001 T3701 T3699 arg1Of NaCl,8.07
R3002 T3701 T3700 arg1Of NaCl,g/l
R3003 T3702 T3706 arg1Of ",",and
R3004 T3703 T3704 arg1Of 0.2,g/l
R3005 T3705 T3702 arg2Of KCl,","
R3006 T3705 T3703 arg1Of KCl,0.2
R3007 T3706 T3708 arg1Of and,µl/l
R3008 T3707 T3706 arg2Of 500,and
R3009 T3708 T3690 arg2Of µl/l,(
R3010 T3708 T3710 arg1Of µl/l,in
R3011 T3709 T3708 arg2Of Tween-20,µl/l
R3012 T3711 T3710 arg2Of H2O,in
R3013 T3712 T3690 arg3Of ),(
R3014 T3713 T3674 arg2Of and,were
R3015 T3713 T3739 arg1Of and,respectively
R3016 T3715 T3713 arg2Of incubated,and
R3017 T3715 T3714 arg1Of incubated,subsequently
R3018 T3715 T3716 arg1Of incubated,with
R3019 T3715 T3738 arg1Of incubated,","
R3020 T3718 T3716 arg2Of antibody,with
R3021 T3718 T3717 arg1Of antibody,primary
R3022 T3718 T3719 arg1Of antibody,as
R3023 T3723 T3721 arg1Of peroxidase-conjugated,and
R3024 T3723 T3722 arg1Of peroxidase-conjugated,horseradish
R3025 T3725 T3720 arg2Of antibody,indicated
R3026 T3725 T3723 arg1Of antibody,peroxidase-conjugated
R3027 T3725 T3724 arg1Of antibody,secondary
R3028 T3725 T3726 arg1Of antibody,","
R3029 T3726 T3719 arg2Of ",",as
R3030 T3726 T3731 arg1Of ",",(
R3031 T3727 T3728 arg1Of anti-mouse,or
R3032 T3729 T3728 arg2Of anti-goat,or
R3033 T3730 T3726 arg2Of IgG,","
R3034 T3730 T3727 arg1Of IgG,anti-mouse
R3035 T3730 T3729 arg1Of IgG,anti-goat
R3036 T3732 T3733 arg1Of DAKO,","
R3037 T3733 T3735 arg1Of ",",","
R3038 T3734 T3733 arg2Of Hamburg,","
R3039 T3735 T3731 arg2Of ",",(
R3040 T3736 T3735 arg2Of Germany,","
R3041 T3737 T3731 arg3Of ),(
R3042 T3741 T3740 arg1Of membranes,The
R3043 T3741 T3742 arg1Of membranes,were
R3044 T3741 T3744 arg2Of membranes,developed
R3045 T3744 T3742 arg2Of developed,were
R3046 T3744 T3743 arg1Of developed,then
R3047 T3744 T3745 arg1Of developed,with
R3048 T3749 T3745 arg2Of kit,with
R3049 T3749 T3746 arg1Of kit,an
R3050 T3749 T3747 arg1Of kit,ECL
R3051 T3749 T3748 arg1Of kit,detection
R3052 T3749 T3750 arg1Of kit,(
R3053 T3753 T3750 arg2Of Biotech,(
R3054 T3753 T3751 arg1Of Biotech,Amersham
R3055 T3753 T3752 arg1Of Biotech,Pharmacia
R3056 T3753 T3754 arg1Of Biotech,","
R3057 T3755 T3754 arg2Of Freiburg,","
R3058 T3755 T3756 arg1Of Freiburg,","
R3059 T3757 T3756 arg2Of Germany,","
R3060 T3758 T3750 arg3Of ),(
R3061 T3761 T3759 arg1Of antibodies,The
R3062 T3761 T3760 arg1Of antibodies,primary
R3063 T3761 T3762 arg1Of antibodies,were
R3064 T3764 T3763 arg1Of anti-IRF-4/ICSAT,goat
R3065 T3764 T3765 arg1Of anti-IRF-4/ICSAT,(
R3066 T3764 T3768 arg1Of anti-IRF-4/ICSAT,(
R3067 T3764 T3778 arg1Of anti-IRF-4/ICSAT,and
R3068 T3766 T3765 arg2Of M-17,(
R3069 T3767 T3765 arg3Of ),(
R3070 T3771 T3768 arg2Of Biotechnology,(
R3071 T3771 T3769 arg1Of Biotechnology,Santa
R3072 T3771 T3770 arg1Of Biotechnology,Cruz
R3073 T3771 T3772 arg1Of Biotechnology,","
R3074 T3774 T3772 arg2Of Cruz,","
R3075 T3774 T3773 arg1Of Cruz,Santa
R3076 T3774 T3775 arg1Of Cruz,","
R3077 T3776 T3775 arg2Of CA,","
R3078 T3777 T3768 arg3Of ),(
R3079 T3778 T3762 arg2Of and,were
R3080 T3778 T3784 arg1Of and,(
R3081 T3782 T3778 arg2Of AC-74,and
R3082 T3782 T3779 arg1Of AC-74,mouse
R3083 T3782 T3780 arg1Of AC-74,anti-β-actin
R3084 T3782 T3781 arg2Of AC-74,(
R3085 T3783 T3781 arg3Of ),(
R3086 T3785 T3784 arg2Of Sigma,(
R3087 T3786 T3784 arg3Of ),(
R3523 T4372 T4371 arg1Of restriction-PCR-assay,Methylation-specific
R3524 T4373 T4374 arg1Of DNA,was
R3525 T4373 T4375 arg2Of DNA,extracted
R3526 T4375 T4374 arg2Of extracted,was
R3527 T4375 T4376 arg1Of extracted,with
R3528 T4375 T4383 arg1Of extracted,as
R3529 T4379 T4376 arg2Of kit,with
R3530 T4379 T4377 arg1Of kit,a
R3531 T4379 T4378 arg1Of kit,commercial
R3532 T4379 T4380 arg1Of kit,(
R3533 T4381 T4380 arg2Of Qiagen,(
R3534 T4382 T4380 arg3Of ),(
R3535 T4384 T4383 arg2Of recommended,as
R3536 T4388 T4386 arg1Of ability,the
R3537 T4388 T4387 arg1Of ability,restriction
R3538 T4388 T4389 arg1Of ability,of
R3539 T4388 T4392 arg1Of ability,is
R3540 T4388 T4393 arg2Of ability,inhibited
R3541 T4391 T4389 arg2Of endonucleases,of
R3542 T4391 T4390 arg1Of endonucleases,several
R3543 T4393 T4385 arg2Of inhibited,Since
R3544 T4393 T4392 arg2Of inhibited,is
R3545 T4395 T4393 arg1Of methylation,inhibited
R3546 T4395 T4394 arg2Of methylation,by
R3547 T4395 T4396 arg1Of methylation,of
R3548 T4399 T4396 arg2Of sequence,of
R3549 T4399 T4397 arg1Of sequence,their
R3550 T4399 T4398 arg1Of sequence,target
R3551 T4401 T4402 arg1Of we,used
R3552 T4402 T4385 arg1Of used,Since
R3553 T4402 T4400 arg1Of used,","
R3554 T4405 T4403 arg1Of HpaII,methylation-sensitive
R3555 T4405 T4404 arg1Of HpaII,enzymes
R3556 T4405 T4406 arg1Of HpaII,and
R3557 T4406 T4409 arg1Of and,and
R3558 T4408 T4406 arg2Of Bsp143II,and
R3559 T4408 T4407 arg1Of Bsp143II,HaeII-isochizomer
R3560 T4409 T4402 arg2Of and,used
R3561 T4409 T4420 arg1Of and,(
R3562 T4410 T4409 arg2Of Bsh1236I,and
R3563 T4410 T4411 arg1Of Bsh1236I,(
R3564 T4413 T4411 arg2Of Fermentas,(
R3565 T4413 T4412 arg1Of Fermentas,MBI
R3566 T4413 T4414 arg1Of Fermentas,","
R3567 T4416 T4414 arg2Of Leon-Rot,","
R3568 T4416 T4415 arg1Of Leon-Rot,St
R3569 T4416 T4417 arg1Of Leon-Rot,","
R3570 T4418 T4417 arg2Of Germany,","
R3571 T4419 T4411 arg3Of ),(
R3572 T4421 T4420 arg2Of "20,24",(
R3573 T4422 T4420 arg3Of ),(
R3574 T4424 T4423 arg2Of control,As
R3575 T4424 T4432 arg1Of control,with
R3576 T4428 T4425 arg1Of MspI,the
R3577 T4428 T4426 arg1Of MspI,methylation-resistant
R3578 T4428 T4427 arg1Of MspI,enzyme
R3579 T4428 T4429 arg1Of MspI,and
R3580 T4429 T4424 arg2Of and,control
R3581 T4431 T4429 arg2Of enzyme,and
R3582 T4431 T4430 arg1Of enzyme,an
R3583 T4435 T4432 arg2Of site,with
R3584 T4435 T4433 arg1Of site,no
R3585 T4435 T4434 arg1Of site,recognition
R3586 T4435 T4436 arg1Of site,in
R3587 T4439 T4436 arg2Of promoter,in
R3588 T4439 T4437 arg1Of promoter,the
R3589 T4439 T4438 arg1Of promoter,target
R3590 T4441 T4443 arg1Of EcoRI,were
R3591 T4441 T4444 arg2Of EcoRI,used
R3592 T4444 T4423 arg1Of used,As
R3593 T4444 T4440 arg1Of used,","
R3594 T4444 T4442 arg1Of used,","
R3595 T4444 T4443 arg2Of used,were
R3596 T4445 T4446 arg1Of DNA,(
R3597 T4445 T4450 arg1Of DNA,was
R3598 T4445 T4451 arg2Of DNA,digested
R3599 T4448 T4446 arg2Of µg,(
R3600 T4448 T4447 arg1Of µg,0.8
R3601 T4449 T4446 arg3Of ),(
R3602 T4451 T4450 arg2Of digested,was
R3603 T4451 T4462 arg1Of digested,","
R3604 T4451 T4463 modOf digested,to
R3605 T4451 T4467 arg1Of digested,","
R3606 T4451 T4471 arg1Of digested,for
R3607 T4454 T4453 arg1Of U,40
R3608 T4457 T4451 arg1Of enzyme,digested
R3609 T4457 T4452 arg2Of enzyme,by
R3610 T4457 T4454 arg1Of enzyme,U
R3611 T4457 T4455 arg1Of enzyme,the
R3612 T4457 T4456 arg1Of enzyme,respective
R3613 T4457 T4458 arg1Of enzyme,for
R3614 T4457 T4461 arg1Of enzyme,and
R3615 T4460 T4458 arg2Of h,for
R3616 T4460 T4459 arg1Of h,6
R3617 T4464 T4463 arg1Of ensure,to
R3618 T4466 T4464 arg2Of cleavage,ensure
R3619 T4466 T4465 arg1Of cleavage,complete
R3620 T4470 T4468 arg1Of U,additional
R3621 T4470 T4469 arg1Of U,20
R3622 T4471 T4470 arg1Of for,U
R3623 T4473 T4471 arg2Of h,for
R3624 T4473 T4472 arg1Of h,16
R3625 T4475 T4474 arg1Of 100,Thereafter
R3626 T4476 T4475 arg1Of ng,100
R3627 T4476 T4477 arg1Of ng,of
R3628 T4476 T4480 arg1Of ng,was
R3629 T4476 T4481 arg2Of ng,used
R3630 T4479 T4477 arg2Of DNA,of
R3631 T4479 T4478 arg1Of DNA,digested
R3632 T4481 T4480 arg2Of used,was
R3633 T4481 T4482 arg1Of used,to
R3634 T4481 T4510 arg1Of used,)
R3635 T4485 T4482 arg2Of amplification,to
R3636 T4485 T4483 arg1Of amplification,a
R3637 T4485 T4484 arg1Of amplification,PCR
R3638 T4485 T4486 arg1Of amplification,of
R3639 T4485 T4489 arg1Of amplification,(
R3640 T4485 T4494 arg1Of amplification,spanning
R3641 T4485 T4507 modOf amplification,see
R3642 T4488 T4486 arg2Of fragments,of
R3643 T4488 T4487 arg1Of fragments,two
R3644 T4490 T4491 arg1Of F1,and
R3645 T4491 T4489 arg2Of and,(
R3646 T4492 T4491 arg2Of F2,and
R3647 T4493 T4489 arg3Of ),(
R3648 T4494 T4506 arg1Of spanning,;
R3649 T4495 T4494 arg2Of part,spanning
R3650 T4495 T4496 arg1Of part,of
R3651 T4501 T4500 arg2Of 30,(
R3652 T4502 T4500 arg3Of ),(
R3653 T4505 T4496 arg2Of U52683,of
R3654 T4505 T4497 arg1Of U52683,the
R3655 T4505 T4498 arg1Of U52683,IRF-4
R3656 T4505 T4499 arg1Of U52683,promoter
R3657 T4505 T4500 arg1Of U52683,(
R3658 T4505 T4503 arg1Of U52683,(
R3659 T4505 T4504 arg1Of U52683,GenBank
R3660 T4509 T4507 arg2Of 3A,see
R3661 T4509 T4508 arg1Of 3A,Figure
R3662 T4512 T4511 arg1Of sequences,The
R3663 T4512 T4513 arg1Of sequences,of
R3664 T4512 T4516 arg1Of sequences,were
R3665 T4512 T4517 arg1Of sequences,F1-forward
R3666 T4515 T4513 arg2Of primers,of
R3667 T4515 T4514 arg1Of primers,the
R3668 T4516 T4518 arg1Of were,:
R3669 T4517 T4516 arg2Of F1-forward,were
R3670 T4519 T4520 arg1Of 5′-TTGAGATGGAGTCTTGCTCTGT-3′,","
R3671 T4520 T4522 arg1Of ",",:
R3672 T4521 T4520 arg2Of F1-reverse,","
R3673 T4523 T4519 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,5′-TTGAGATGGAGTCTTGCTCTGT-3′
R3674 T4523 T4521 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,F1-reverse
R3675 T4523 T4524 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,(
R3676 T4523 T4528 arg1Of ATCACTTCCAGACTTCAGTTCACCT-3′,;
R3677 T4526 T4524 arg2Of bp,(
R3678 T4526 T4525 arg1Of bp,341
R3679 T4527 T4524 arg3Of ),(
R3680 T4529 T4528 arg2Of F2-forward,;
R3681 T4529 T4530 arg1Of F2-forward,:
R3682 T4531 T4532 arg1Of 5′-AAGGTGAACTGAAGTCTGGAAGTGA-3′,","
R3683 T4532 T4534 arg1Of ",",:
R3684 T4533 T4532 arg2Of F2-reverse,","
R3685 T4535 T4536 arg1Of 5′-CCAGGACCTCAGGAGGCCAGTCA-3′,(
R3686 T4538 T4536 arg2Of bp,(
R3687 T4538 T4537 arg1Of bp,474
R3688 T4539 T4536 arg3Of ),(
R3689 T4542 T4540 arg1Of conditions,The
R3690 T4542 T4541 arg1Of conditions,PCR
R3691 T4542 T4543 arg1Of conditions,were
R3692 T4542 T4544 arg2Of conditions,described
R3693 T4544 T4543 arg2Of described,were
R3694 T4544 T4545 arg1Of described,elsewhere
R3695 T4545 T4546 arg1Of elsewhere,(
R3696 T4547 T4546 arg2Of 3,(
R3697 T4548 T4546 arg3Of ),(
R3698 T4549 T4550 arg1Of PCR,was
R3699 T4549 T4551 arg2Of PCR,performed
R3700 T4551 T4550 arg2Of performed,was
R3701 T4551 T4552 arg1Of performed,with
R3702 T4555 T4552 arg2Of temperature,with
R3703 T4555 T4553 arg1Of temperature,an
R3704 T4555 T4554 arg1Of temperature,annealing
R3705 T4555 T4556 arg1Of temperature,of
R3706 T4557 T4558 arg1Of 62°C,and
R3707 T4558 T4556 arg2Of and,of
R3708 T4560 T4558 arg2Of cycles,and
R3709 T4560 T4559 arg1Of cycles,35
R3710 T4562 T4563 arg1Of DNA,was
R3711 T4562 T4564 arg2Of DNA,methylated
R3712 T4564 T4561 arg2Of methylated,When
R3713 T4564 T4563 arg2Of methylated,was
R3714 T4564 T4565 arg1Of methylated,at
R3715 T4567 T4565 arg2Of sites,at
R3716 T4567 T4566 arg1Of sites,specific
R3717 T4571 T4569 arg1Of enzymes,the
R3718 T4571 T4570 arg1Of enzymes,sensitive
R3719 T4571 T4572 arg1Of enzymes,were
R3720 T4571 T4574 arg1Of enzymes,able
R3721 T4571 T4576 arg1Of enzymes,digest
R3722 T4572 T4573 arg1Of were,not
R3723 T4572 T4579 arg1Of were,and
R3724 T4574 T4572 arg2Of able,were
R3725 T4576 T4574 arg2Of digest,able
R3726 T4576 T4575 arg1Of digest,to
R3727 T4578 T4576 arg2Of DNA,digest
R3728 T4578 T4577 arg1Of DNA,the
R3729 T4579 T4561 arg1Of and,When
R3730 T4579 T4568 arg1Of and,","
R3731 T4579 T4583 arg1Of and,;
R3732 T4580 T4581 arg1Of amplification,took
R3733 T4581 T4579 arg2Of took,and
R3734 T4582 T4581 arg2Of place,took
R3735 T4585 T4584 arg2Of case,in
R3736 T4585 T4586 arg1Of case,of
R3737 T4588 T4586 arg2Of methylation,of
R3738 T4588 T4587 arg1Of methylation,no
R3739 T4590 T4591 arg1Of DNA,was
R3740 T4590 T4592 arg2Of DNA,digested
R3741 T4592 T4591 arg2Of digested,was
R3742 T4592 T4593 arg1Of digested,and
R3743 T4593 T4583 arg2Of and,;
R3744 T4593 T4584 arg1Of and,in
R3745 T4593 T4589 arg1Of and,","
R3746 T4595 T4594 arg1Of product,no
R3747 T4595 T4596 arg1Of product,was
R3748 T4595 T4597 arg2Of product,generated
R3749 T4597 T4593 arg2Of generated,and
R3750 T4597 T4596 arg2Of generated,was
R3751 T4600 T4598 arg1Of products,The
R3752 T4600 T4599 arg1Of products,PCR
R3753 T4600 T4601 arg1Of products,were
R3754 T4600 T4602 arg2Of products,electrophoresed
R3755 T4600 T4610 arg1Of products,were
R3756 T4600 T4611 arg2Of products,stained
R3757 T4600 T4616 arg2Of products,photographed
R3758 T4602 T4601 arg2Of electrophoresed,were
R3759 T4602 T4603 arg1Of electrophoresed,on
R3760 T4602 T4609 arg1Of electrophoresed,","
R3761 T4605 T4606 arg1Of 3,%
R3762 T4608 T4603 arg2Of gel,on
R3763 T4608 T4604 arg1Of gel,a
R3764 T4608 T4605 arg1Of gel,3
R3765 T4608 T4607 arg1Of gel,agarose
R3766 T4611 T4612 arg1Of stained,with
R3767 T4611 T4615 arg1Of stained,and
R3768 T4614 T4612 arg2Of bromide,with
R3769 T4614 T4613 arg1Of bromide,ethidium
R3770 T4615 T4609 arg2Of and,","
R3771 T4615 T4610 arg2Of and,were
R3772 T4616 T4615 arg2Of photographed,and
R3773 T4618 T4617 arg1Of products,PCR
R3774 T4618 T4619 arg1Of products,were
R3775 T4618 T4620 arg2Of products,verified
R3776 T4620 T4619 arg2Of verified,were
R3777 T4623 T4620 arg1Of sequencing,verified
R3778 T4623 T4621 arg2Of sequencing,by
R3779 T4623 T4622 arg2Of sequencing,automated
R4058 T4944 T4943 arg1Of treatment,Bisulfite
R4059 T4945 T4946 arg1Of DNA,was
R4060 T4945 T4947 arg2Of DNA,extracted
R4061 T4947 T4946 arg2Of extracted,was
R4062 T4947 T4948 arg1Of extracted,as
R4063 T4949 T4948 arg2Of described,as
R4064 T4949 T4950 arg1Of described,above
R4065 T4952 T4951 arg1Of treatment,Bisulfite
R4066 T4952 T4953 arg1Of treatment,of
R4067 T4952 T4955 arg1Of treatment,","
R4068 T4952 T4956 arg1Of treatment,leading
R4069 T4952 T4962 modOf treatment,to
R4070 T4952 T4978 arg1Of treatment,follows
R4071 T4954 T4953 arg2Of DNA,of
R4072 T4956 T4957 arg1Of leading,to
R4073 T4958 T4957 arg2Of conversion,to
R4074 T4958 T4959 arg1Of conversion,of
R4075 T4961 T4959 arg2Of cytosine,of
R4076 T4961 T4960 arg1Of cytosine,unmethylated
R4077 T4963 T4972 arg1Of uracil,","
R4078 T4964 T4965 arg1Of residues,and
R4079 T4965 T4963 arg2Of and,uracil
R4080 T4967 T4965 arg2Of change,and
R4081 T4967 T4966 arg1Of change,no
R4082 T4967 T4968 arg1Of change,of
R4083 T4971 T4968 arg2Of residues,of
R4084 T4971 T4969 arg2Of residues,methylated
R4085 T4971 T4970 arg1Of residues,cytosine
R4086 T4972 T4962 arg1Of ",",to
R4087 T4974 T4972 arg2Of performed,","
R4088 T4974 T4973 arg2Of performed,was
R4089 T4974 T4975 arg1Of performed,as
R4090 T4976 T4975 arg2Of described,as
R4091 T4976 T4977 arg1Of described,as
R4092 T4982 T4981 arg1Of µg,1
R4093 T4982 T4983 arg1Of µg,of
R4094 T4982 T4988 arg1Of µg,of
R4095 T4982 T5004 arg1Of µg,were
R4096 T4982 T5005 arg2Of µg,denaturated
R4097 T4984 T4985 arg1Of DNA,and
R4098 T4985 T4983 arg2Of and,of
R4099 T4987 T4985 arg2Of µg,and
R4100 T4987 T4986 arg1Of µg,2
R4101 T4989 T4990 arg1Of poly,(
R4102 T4991 T4990 arg2Of dA–dT,(
R4103 T4992 T4990 arg3Of ),(
R4104 T4996 T4995 arg2Of dA–dT,(
R4105 T4997 T4995 arg3Of ),(
R4106 T4998 T4988 arg2Of copolymers,of
R4107 T4998 T4989 arg1Of copolymers,poly
R4108 T4998 T4993 arg1Of copolymers,(
R4109 T4998 T4994 arg1Of copolymers,poly
R4110 T4998 T4995 arg1Of copolymers,(
R4111 T4998 T4999 arg1Of copolymers,(
R4112 T5002 T4999 arg2Of Biotech,(
R4113 T5002 T5000 arg1Of Biotech,Amersham
R4114 T5002 T5001 arg1Of Biotech,Pharmacia
R4115 T5003 T4999 arg3Of ),(
R4116 T5005 T4979 arg1Of denaturated,Briefly
R4117 T5005 T4980 arg1Of denaturated,","
R4118 T5005 T5004 arg2Of denaturated,were
R4119 T5005 T5006 arg1Of denaturated,for
R4120 T5005 T5009 arg1Of denaturated,at
R4121 T5005 T5011 arg1Of denaturated,in
R4122 T5005 T5015 arg1Of denaturated,in
R4123 T5008 T5006 arg2Of min,for
R4124 T5008 T5007 arg1Of min,20
R4125 T5010 T5009 arg2Of 42°C,at
R4126 T5012 T5013 arg1Of 0.3,M
R4127 T5014 T5011 arg2Of NaOH,in
R4128 T5014 T5012 arg1Of NaOH,0.3
R4129 T5017 T5015 arg2Of volume,in
R4130 T5017 T5016 arg1Of volume,a
R4131 T5017 T5018 arg1Of volume,of
R4132 T5020 T5018 arg2Of µl,of
R4133 T5020 T5019 arg1Of µl,50
R4134 T5022 T5021 arg1Of solutions,Fresh
R4135 T5022 T5023 arg1Of solutions,of
R4136 T5022 T5047 arg1Of solutions,were
R4137 T5022 T5048 arg2Of solutions,added
R4138 T5025 T5024 arg1Of µl,30
R4139 T5025 T5026 arg1Of µl,of
R4140 T5025 T5033 arg1Of µl,and
R4141 T5027 T5028 arg1Of 10,mM
R4142 T5029 T5026 arg2Of hydrochinon,of
R4143 T5029 T5027 arg1Of hydrochinon,10
R4144 T5029 T5030 arg1Of hydrochinon,(
R4145 T5031 T5030 arg2Of Sigma,(
R4146 T5032 T5030 arg3Of ),(
R4147 T5033 T5023 arg2Of and,of
R4148 T5035 T5033 arg2Of µl,and
R4149 T5035 T5034 arg1Of µl,530
R4150 T5035 T5036 arg1Of µl,of
R4151 T5037 T5038 arg1Of 3,M
R4152 T5040 T5036 arg2Of bisulfite,of
R4153 T5040 T5037 arg1Of bisulfite,3
R4154 T5040 T5039 arg1Of bisulfite,sodium
R4155 T5040 T5041 arg1Of bisulfite,(
R4156 T5042 T5041 arg2Of pH,(
R4157 T5042 T5043 arg1Of pH,5.0
R4158 T5042 T5044 arg1Of pH,;
R4159 T5045 T5044 arg2Of Sigma,;
R4160 T5046 T5041 arg3Of ),(
R4161 T5048 T5047 arg2Of added,were
R4162 T5051 T5050 arg1Of solution,the
R4163 T5051 T5052 arg1Of solution,was
R4164 T5051 T5054 arg2Of solution,mixed
R4165 T5051 T5056 arg2Of solution,overlayed
R4166 T5051 T5061 arg2Of solution,incubated
R4167 T5054 T5055 arg1Of mixed,","
R4168 T5055 T5060 arg1Of ",",and
R4169 T5056 T5055 arg2Of overlayed,","
R4170 T5056 T5057 arg1Of overlayed,with
R4171 T5059 T5057 arg2Of oil,with
R4172 T5059 T5058 arg1Of oil,mineral
R4173 T5060 T5049 arg1Of and,","
R4174 T5060 T5052 arg2Of and,was
R4175 T5060 T5053 arg1Of and,gently
R4176 T5061 T5060 arg2Of incubated,and
R4177 T5061 T5062 arg1Of incubated,in
R4178 T5064 T5062 arg2Of dark,in
R4179 T5064 T5063 arg1Of dark,the
R4180 T5067 T5066 arg1Of h,12–13
R4181 T5067 T5068 arg1Of h,at
R4182 T5067 T5073 arg1Of h,was
R4183 T5067 T5074 arg2Of h,recovered
R4184 T5072 T5068 arg2Of phase,at
R4185 T5072 T5069 arg1Of phase,50°C.
R4186 T5072 T5070 arg1Of phase,The
R4187 T5072 T5071 arg1Of phase,aqueous
R4188 T5074 T5073 arg2Of recovered,was
R4189 T5074 T5075 modOf recovered,using
R4190 T5074 T5081 arg1Of recovered,(
R4191 T5080 T5075 arg2Of system’,using
R4192 T5080 T5076 arg1Of system’,the
R4193 T5080 T5077 arg1Of system’,‘Wizard
R4194 T5080 T5078 arg1Of system’,DNA
R4195 T5080 T5079 arg1Of system’,clean-up
R4196 T5082 T5083 arg1Of Promega,","
R4197 T5083 T5081 arg2Of ",",(
R4198 T5083 T5085 arg1Of ",",","
R4199 T5084 T5083 arg2Of Mannheim,","
R4200 T5086 T5085 arg2Of Germany,","
R4201 T5087 T5081 arg3Of ),(
R4202 T5090 T5088 arg1Of DNA,The
R4203 T5090 T5089 arg2Of DNA,purified
R4204 T5090 T5091 arg1Of DNA,was
R4205 T5090 T5093 arg2Of DNA,mixed
R4206 T5090 T5106 arg2Of DNA,incubated
R4207 T5093 T5094 arg1Of mixed,with
R4208 T5093 T5105 arg1Of mixed,and
R4209 T5095 T5096 arg1Of 1,M
R4210 T5097 T5094 arg2Of NaOH,with
R4211 T5097 T5095 arg1Of NaOH,1
R4212 T5097 T5098 arg1Of NaOH,to
R4213 T5101 T5098 arg2Of concentration,to
R4214 T5101 T5099 arg1Of concentration,a
R4215 T5101 T5100 arg1Of concentration,final
R4216 T5101 T5102 arg1Of concentration,of
R4217 T5104 T5102 arg2Of M,of
R4218 T5104 T5103 arg1Of M,0.3
R4219 T5105 T5091 arg2Of and,was
R4220 T5105 T5092 arg1Of and,subsequently
R4221 T5106 T5105 arg2Of incubated,and
R4222 T5106 T5107 arg1Of incubated,for
R4223 T5106 T5110 arg1Of incubated,at
R4224 T5106 T5112 modOf incubated,to
R4225 T5109 T5107 arg2Of min,for
R4226 T5109 T5108 arg1Of min,20
R4227 T5111 T5110 arg2Of 37°C,at
R4228 T5113 T5112 arg1Of ensure,to
R4229 T5115 T5113 arg2Of desulfonisation,ensure
R4230 T5115 T5114 arg1Of desulfonisation,complete
R4231 T5116 T5117 arg1Of DNA,was
R4232 T5116 T5138 arg1Of DNA,resuspended
R4233 T5117 T5137 arg1Of was,and
R4234 T5118 T5117 arg2Of ethanol,was
R4235 T5118 T5119 arg2Of ethanol,precipitated
R4236 T5119 T5120 arg1Of precipitated,in
R4237 T5122 T5120 arg2Of presence,in
R4238 T5122 T5121 arg1Of presence,the
R4239 T5122 T5123 arg1Of presence,of
R4240 T5122 T5131 arg1Of presence,","
R4241 T5122 T5132 arg2Of presence,washed
R4242 T5125 T5123 arg2Of vol,of
R4243 T5125 T5124 arg1Of vol,1/10
R4244 T5125 T5126 arg1Of vol,of
R4245 T5127 T5128 arg1Of 3,M
R4246 T5130 T5126 arg2Of acetate,of
R4247 T5130 T5127 arg1Of acetate,3
R4248 T5130 T5129 arg1Of acetate,sodium
R4249 T5132 T5133 arg1Of washed,with
R4250 T5134 T5135 arg1Of 70,%
R4251 T5136 T5133 arg2Of ethanol,with
R4252 T5136 T5134 arg1Of ethanol,70
R4253 T5138 T5137 arg2Of resuspended,and
R4254 T5138 T5139 arg1Of resuspended,in
R4255 T5142 T5139 arg2Of H2O,in
R4256 T5142 T5140 arg1Of H2O,50
R4257 T5142 T5141 arg1Of H2O,µl
R4258 T5145 T5143 arg1Of amplification,Subsequent
R4259 T5145 T5144 arg1Of amplification,PCR
R4260 T5145 T5146 arg1Of amplification,of
R4261 T5145 T5151 arg1Of amplification,was
R4262 T5145 T5152 arg2Of amplification,used
R4263 T5150 T5146 arg2Of DNA,of
R4264 T5150 T5147 arg1Of DNA,4
R4265 T5150 T5148 arg1Of DNA,µl
R4266 T5150 T5149 arg1Of DNA,bisulfite-treated
R4267 T5152 T5151 arg2Of used,was
R4268 T5152 T5153 arg1Of used,for
R4269 T5152 T5167 arg1Of used,into
R4270 T5152 T5179 arg1Of used,(
R4271 T5154 T5153 arg2Of cloning,for
R4272 T5154 T5155 arg1Of cloning,of
R4273 T5157 T5155 arg2Of fragments,of
R4274 T5157 T5156 arg1Of fragments,two
R4275 T5157 T5158 arg1Of fragments,of
R4276 T5157 T5162 arg1Of fragments,(
R4277 T5161 T5158 arg2Of promoter,of
R4278 T5161 T5159 arg1Of promoter,the
R4279 T5161 T5160 arg1Of promoter,IRF-4
R4280 T5163 T5164 arg1Of BS-I,and
R4281 T5164 T5162 arg2Of and,(
R4282 T5165 T5164 arg2Of BS-II,and
R4283 T5166 T5162 arg3Of ),(
R4284 T5169 T5167 arg2Of vector,into
R4285 T5169 T5168 arg1Of vector,pCR2.1
R4286 T5169 T5170 arg1Of vector,with
R4287 T5175 T5170 arg2Of kit’,with
R4288 T5175 T5171 arg1Of kit’,the
R4289 T5175 T5172 arg1Of kit’,‘TOPO
R4290 T5175 T5173 arg1Of kit’,TA
R4291 T5175 T5174 arg1Of kit’,cloning
R4292 T5175 T5176 arg1Of kit’,(
R4293 T5177 T5176 arg2Of Invitrogen,(
R4294 T5178 T5176 arg3Of ),(
R4295 T5180 T5179 arg2Of see,(
R4296 T5182 T5180 arg2Of 3A,see
R4297 T5182 T5181 arg1Of 3A,Figure
R4298 T5183 T5179 arg3Of ),(
R4299 T5185 T5184 arg1Of primers,The
R4300 T5185 T5186 arg2Of primers,used
R4301 T5185 T5196 arg1Of primers,contain
R4302 T5186 T5187 arg1Of used,for
R4303 T5189 T5187 arg2Of amplification,for
R4304 T5189 T5188 arg1Of amplification,PCR
R4305 T5189 T5190 arg1Of amplification,of
R4306 T5192 T5193 arg1Of BS-I,and
R4307 T5194 T5193 arg2Of BS-II,and
R4308 T5195 T5190 arg2Of fragments,of
R4309 T5195 T5191 arg1Of fragments,the
R4310 T5195 T5192 arg1Of fragments,BS-I
R4311 T5195 T5194 arg1Of fragments,BS-II
R4312 T5196 T5219 arg1Of contain,:
R4313 T5196 T5221 arg1Of contain,5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′
R4314 T5196 T5229 arg1Of contain,;
R4315 T5196 T5231 arg1Of contain,5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′
R4316 T5200 T5196 arg2Of sequence,contain
R4317 T5200 T5197 arg1Of sequence,the
R4318 T5200 T5198 arg1Of sequence,putative
R4319 T5200 T5199 arg1Of sequence,altered
R4320 T5200 T5201 arg1Of sequence,of
R4321 T5204 T5201 arg2Of strand,of
R4322 T5204 T5202 arg1Of strand,the
R4323 T5204 T5203 arg1Of strand,sense
R4324 T5204 T5205 arg1Of strand,due
R4325 T5207 T5205 arg2Of bisulfite,due
R4326 T5207 T5206 arg1Of bisulfite,to
R4327 T5208 T5207 arg2Of treatment,bisulfite
R4328 T5208 T5209 arg1Of treatment,(
R4329 T5212 T5210 arg2Of residues,converted
R4330 T5212 T5211 arg1Of residues,cytosine
R4331 T5212 T5213 arg1Of residues,are
R4332 T5212 T5214 arg2Of residues,written
R4333 T5214 T5209 arg2Of written,(
R4334 T5214 T5213 arg2Of written,are
R4335 T5214 T5215 arg1Of written,in
R4336 T5217 T5215 arg2Of letters,in
R4337 T5217 T5216 arg1Of letters,bold
R4338 T5218 T5209 arg3Of ),(
R4339 T5221 T5220 arg1Of 5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′,BS-I-forward
R4340 T5221 T5222 arg1Of 5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′,","
R4341 T5224 T5222 arg2Of 5′-ACCCAACTCCCTTAAACTATTAAACT-3′,","
R4342 T5224 T5223 arg1Of 5′-ACCCAACTCCCTTAAACTATTAAACT-3′,BS-I-reverse
R4343 T5224 T5225 arg1Of 5′-ACCCAACTCCCTTAAACTATTAAACT-3′,(
R4344 T5227 T5225 arg2Of bp,(
R4345 T5227 T5226 arg1Of bp,187
R4346 T5228 T5225 arg3Of ),(
R4347 T5231 T5230 arg1Of 5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′,BS-II-forward
R4348 T5231 T5232 arg1Of 5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′,","
R4349 T5234 T5232 arg2Of 5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,","
R4350 T5234 T5233 arg1Of 5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,BS-II-reverse
R4351 T5234 T5235 arg1Of 5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,(
R4352 T5237 T5235 arg2Of bp,(
R4353 T5237 T5236 arg1Of bp,674
R4354 T5238 T5235 arg3Of ),(
R4355 T5241 T5239 arg2Of amplification,After
R4356 T5241 T5240 arg1Of amplification,bacterial
R4357 T5241 T5242 arg1Of amplification,of
R4358 T5241 T5247 arg1Of amplification,by
R4359 T5246 T5242 arg2Of fragments,of
R4360 T5246 T5243 arg1Of fragments,the
R4361 T5246 T5244 arg2Of fragments,cloned
R4362 T5246 T5245 arg1Of fragments,PCR
R4363 T5249 T5247 arg2Of procedures,by
R4364 T5249 T5248 arg1Of procedures,standard
R4365 T5252 T5251 arg1Of clones,eight
R4366 T5252 T5253 arg1Of clones,from
R4367 T5252 T5256 arg1Of clones,were
R4368 T5252 T5257 arg2Of clones,sequenced
R4369 T5255 T5253 arg2Of sample,from
R4370 T5255 T5254 arg1Of sample,each
R4371 T5257 T5239 arg1Of sequenced,After
R4372 T5257 T5250 arg1Of sequenced,","
R4373 T5257 T5256 arg2Of sequenced,were
R4374 T5257 T5258 arg1Of sequenced,with
R4375 T5257 T5262 arg1Of sequenced,(
R4376 T5261 T5258 arg2Of sequencer,with
R4377 T5261 T5259 arg1Of sequencer,an
R4378 T5261 T5260 arg2Of sequencer,automated
R4379 T5264 T5262 arg2Of Prism,(
R4380 T5264 T5263 arg1Of Prism,ABI
R4381 T5264 T5265 arg1Of Prism,377
R4382 T5264 T5266 arg1Of Prism,","
R4383 T5268 T5266 arg2Of Biosystems,","
R4384 T5268 T5267 arg1Of Biosystems,Applied
R4385 T5269 T5262 arg3Of ),(
R4762 T5702 T5701 arg1Of vitro,In
R4763 T5703 T5704 arg1Of methylation,and
R4764 T5705 T5704 arg2Of reporter,and
R4765 T5707 T5702 arg1Of assays,vitro
R4766 T5707 T5703 arg1Of assays,methylation
R4767 T5707 T5705 arg1Of assays,reporter
R4768 T5707 T5706 arg1Of assays,gene
R4769 T5712 T5708 arg1Of construct,The
R4770 T5712 T5709 arg1Of construct,IRF-4
R4771 T5712 T5710 arg1Of construct,promoter-reporter
R4772 T5712 T5711 arg1Of construct,gene
R4773 T5712 T5713 arg1Of construct,was
R4774 T5712 T5715 arg2Of construct,provided
R4775 T5715 T5713 arg2Of provided,was
R4776 T5715 T5714 arg1Of provided,generously
R4777 T5718 T5715 arg1Of Hiscott,provided
R4778 T5718 T5716 arg2Of Hiscott,by
R4779 T5718 T5717 arg1Of Hiscott,J.
R4780 T5718 T5719 arg1Of Hiscott,(
R4781 T5720 T5719 arg2Of 31,(
R4782 T5721 T5719 arg3Of ),(
R4783 T5722 T5723 arg1Of Constructs,were
R4784 T5722 T5724 arg2Of Constructs,methylated
R4785 T5724 T5723 arg2Of methylated,were
R4786 T5724 T5726 arg1Of methylated,vitro
R4787 T5724 T5727 arg1Of methylated,with
R4788 T5724 T5734 arg1Of methylated,as
R4789 T5724 T5743 arg1Of methylated,and
R4790 T5726 T5725 arg1Of vitro,in
R4791 T5729 T5727 arg2Of Methylase,with
R4792 T5729 T5728 arg1Of Methylase,CpG
R4793 T5729 T5730 arg1Of Methylase,(
R4794 T5731 T5730 arg2Of M.Sss,(
R4795 T5731 T5732 arg1Of M.Sss,I
R4796 T5733 T5730 arg3Of ),(
R4797 T5735 T5734 arg2Of recommended,as
R4798 T5738 T5735 arg1Of manufacturer,recommended
R4799 T5738 T5736 arg2Of manufacturer,by
R4800 T5738 T5737 arg1Of manufacturer,the
R4801 T5738 T5739 arg1Of manufacturer,(
R4802 T5741 T5739 arg2Of Biolabs,(
R4803 T5741 T5740 arg1Of Biolabs,NE
R4804 T5742 T5739 arg3Of ),(
R4805 T5745 T5744 arg1Of methylation,complete
R4806 T5745 T5746 arg1Of methylation,was
R4807 T5745 T5747 arg2Of methylation,checked
R4808 T5747 T5743 arg2Of checked,and
R4809 T5747 T5746 arg2Of checked,was
R4810 T5747 T5748 arg1Of checked,via
R4811 T5750 T5748 arg2Of analysis,via
R4812 T5750 T5749 arg1Of analysis,restriction
R4813 T5750 T5751 arg1Of analysis,(
R4814 T5753 T5751 arg2Of 5A,(
R4815 T5753 T5752 arg1Of 5A,Figure
R4816 T5754 T5751 arg3Of ),(
R4817 T5756 T5755 arg1Of gene,Reporter
R4818 T5756 T5766 modOf gene,were
R4819 T5756 T5768 arg1Of gene,similar
R4820 T5757 T5758 arg1Of assays,using
R4821 T5757 T5766 arg1Of assays,were
R4822 T5757 T5767 arg2Of assays,performed
R4823 T5762 T5758 arg2Of assay,using
R4824 T5762 T5759 arg1Of assay,the
R4825 T5762 T5760 arg1Of assay,dual
R4826 T5762 T5761 arg1Of assay,luciferase
R4827 T5762 T5763 arg1Of assay,(
R4828 T5764 T5763 arg2Of Promega,(
R4829 T5765 T5763 arg3Of ),(
R4830 T5767 T5766 arg2Of performed,were
R4831 T5768 T5769 arg1Of similar,to
R4832 T5771 T5756 arg1Of reports,gene
R4833 T5771 T5770 arg1Of reports,previous
R4834 T5771 T5772 arg1Of reports,(
R4835 T5773 T5772 arg2Of 29,(
R4836 T5774 T5772 arg3Of ),(
R4837 T5778 T5777 arg1Of nM,5
R4838 T5778 T5779 arg1Of nM,of
R4839 T5778 T5783 arg1Of nM,and
R4840 T5782 T5779 arg2Of construct,of
R4841 T5782 T5780 arg1Of construct,the
R4842 T5782 T5781 arg1Of construct,reporter
R4843 T5783 T5793 arg1Of and,were
R4844 T5783 T5794 arg2Of and,transientlyco-expressed
R4845 T5787 T5783 arg2Of construct,and
R4846 T5787 T5784 arg1Of construct,the
R4847 T5787 T5785 arg1Of construct,transfection
R4848 T5787 T5786 arg1Of construct,control
R4849 T5787 T5788 arg1Of construct,expressing
R4850 T5792 T5788 arg2Of gene,expressing
R4851 T5792 T5789 arg1Of gene,the
R4852 T5792 T5790 arg1Of gene,renilla
R4853 T5792 T5791 arg1Of gene,luciferase
R4854 T5794 T5775 arg1Of transientlyco-expressed,Briefly
R4855 T5794 T5776 arg1Of transientlyco-expressed,","
R4856 T5794 T5793 arg2Of transientlyco-expressed,were
R4857 T5794 T5795 arg1Of transientlyco-expressed,via
R4858 T5796 T5795 arg2Of electroporation,via
R4859 T5799 T5797 arg1Of construct,The
R4860 T5799 T5798 arg1Of construct,control
R4861 T5799 T5800 arg1Of construct,served
R4862 T5800 T5801 arg1Of served,as
R4863 T5804 T5801 arg2Of reference,as
R4864 T5804 T5802 arg1Of reference,an
R4865 T5804 T5803 arg1Of reference,internal
R4866 T5804 T5805 arg1Of reference,for
R4867 T5807 T5805 arg2Of efficiency,for
R4868 T5807 T5806 arg1Of efficiency,transfection
R4869 T5809 T5808 arg1Of hours,Forty-eight
R4870 T5810 T5809 arg1Of after,hours
R4871 T5811 T5810 arg2Of transfection,after
R4872 T5814 T5813 arg1Of activity,luciferase
R4873 T5814 T5815 arg1Of activity,was
R4874 T5814 T5816 arg2Of activity,measured
R4875 T5816 T5810 arg1Of measured,after
R4876 T5816 T5812 arg1Of measured,","
R4877 T5816 T5815 arg2Of measured,was
R4878 T5816 T5817 arg1Of measured,with
R4879 T5816 T5823 arg1Of measured,(
R4880 T5822 T5817 arg2Of microlumat,with
R4881 T5822 T5818 arg1Of microlumat,a
R4882 T5822 T5819 arg1Of microlumat,LB
R4883 T5822 T5820 arg1Of microlumat,96
R4884 T5822 T5821 arg1Of microlumat,P
R4885 T5827 T5823 arg2Of Berthold,(
R4886 T5827 T5824 arg1Of Berthold,EG
R4887 T5827 T5825 arg1Of Berthold,&
R4888 T5827 T5826 arg1Of Berthold,G
R4889 T5827 T5828 arg1Of Berthold,","
R4890 T5830 T5828 arg2Of Wildbad,","
R4891 T5830 T5829 arg1Of Wildbad,Bad
R4892 T5830 T5831 arg1Of Wildbad,","
R4893 T5832 T5831 arg2Of Germany,","
R4894 T5833 T5823 arg3Of ),(
R4895 T5836 T5834 arg1Of activation,IRF-4
R4896 T5836 T5835 arg1Of activation,promoter
R4897 T5836 T5837 arg1Of activation,was
R4898 T5836 T5838 arg2Of activation,quantified
R4899 T5838 T5837 arg2Of quantified,was
R4900 T5838 T5839 arg1Of quantified,as
R4901 T5841 T5839 arg2Of ratio,as
R4902 T5841 T5840 arg1Of ratio,a
R4903 T5841 T5842 arg1Of ratio,of
R4904 T5845 T5844 arg1Of light,firefly
R4905 T5846 T5842 arg2Of units,of
R4906 T5846 T5843 arg2Of units,measured
R4907 T5846 T5845 arg1Of units,light
R4908 T5846 T5847 arg1Of units,(
R4909 T5846 T5850 arg1Of units,relative
R4910 T5848 T5847 arg2Of flu,(
R4911 T5849 T5847 arg3Of ),(
R4912 T5850 T5851 arg1Of relative,to
R4913 T5852 T5851 arg2Of renilla,to
R4914 T5852 T5853 arg1Of renilla,(
R4915 T5854 T5853 arg2Of rlu,(
R4916 T5855 T5853 arg3Of ),(
R4917 T5857 T5856 arg1Of experiment,Each
R4918 T5857 T5858 arg1Of experiment,was
R4919 T5857 T5859 arg2Of experiment,carried
R4920 T5859 T5858 arg2Of carried,was
R4921 T5859 T5860 arg1Of carried,out
R4922 T5859 T5864 arg1Of carried,times
R4923 T5863 T5861 arg1Of three,at
R4924 T5863 T5862 arg1Of three,least
R4925 T5864 T5863 arg1Of times,three