Id |
Subject |
Object |
Predicate |
Lexical cue |
T4943 |
0-9 |
NN |
denotes |
Bisulfite |
T4944 |
10-19 |
NN |
denotes |
treatment |
T4945 |
20-23 |
NN |
denotes |
DNA |
T4946 |
24-27 |
VB |
denotes |
was |
T4947 |
28-37 |
VB |
denotes |
extracted |
T4948 |
38-40 |
IN |
denotes |
as |
T4949 |
41-50 |
VB |
denotes |
described |
T4950 |
51-56 |
RB |
denotes |
above |
T4951 |
58-67 |
NN |
denotes |
Bisulfite |
T4952 |
68-77 |
NN |
denotes |
treatment |
T4953 |
78-80 |
IN |
denotes |
of |
T4954 |
81-84 |
NN |
denotes |
DNA |
T4955 |
84-85 |
-COMMA- |
denotes |
, |
T4956 |
86-93 |
VB |
denotes |
leading |
T4957 |
94-96 |
TO |
denotes |
to |
T4958 |
97-107 |
NN |
denotes |
conversion |
T4959 |
108-110 |
IN |
denotes |
of |
T4960 |
111-123 |
JJ |
denotes |
unmethylated |
T4961 |
124-132 |
NN |
denotes |
cytosine |
T4962 |
133-135 |
TO |
denotes |
to |
T4963 |
136-142 |
NN |
denotes |
uracil |
T4964 |
143-151 |
NN |
denotes |
residues |
T4965 |
152-155 |
CC |
denotes |
and |
T4966 |
156-158 |
DT |
denotes |
no |
T4967 |
159-165 |
NN |
denotes |
change |
T4968 |
166-168 |
IN |
denotes |
of |
T4969 |
169-179 |
VB |
denotes |
methylated |
T4970 |
180-188 |
NN |
denotes |
cytosine |
T4971 |
189-197 |
NN |
denotes |
residues |
T4972 |
197-198 |
-COMMA- |
denotes |
, |
T4973 |
199-202 |
VB |
denotes |
was |
T4974 |
203-212 |
VB |
denotes |
performed |
T4975 |
213-215 |
IN |
denotes |
as |
T4976 |
216-225 |
VB |
denotes |
described |
T4977 |
226-228 |
IN |
denotes |
as |
T4978 |
229-236 |
VB |
denotes |
follows |
T4979 |
238-245 |
RB |
denotes |
Briefly |
T4980 |
245-246 |
-COMMA- |
denotes |
, |
T4981 |
247-248 |
CD |
denotes |
1 |
T4982 |
249-251 |
NN |
denotes |
µg |
T4983 |
252-254 |
IN |
denotes |
of |
T4984 |
255-258 |
NN |
denotes |
DNA |
T4985 |
259-262 |
CC |
denotes |
and |
T4986 |
263-264 |
CD |
denotes |
2 |
T4987 |
265-267 |
NN |
denotes |
µg |
T4988 |
268-270 |
IN |
denotes |
of |
T4989 |
271-275 |
NN |
denotes |
poly |
T4990 |
275-276 |
-LRB- |
denotes |
( |
T4991 |
276-281 |
NN |
denotes |
dA–dT |
T4992 |
281-282 |
-RRB- |
denotes |
) |
T4993 |
282-283 |
-LRB- |
denotes |
( |
T4994 |
283-287 |
NN |
denotes |
poly |
T4995 |
287-288 |
-LRB- |
denotes |
( |
T4996 |
288-293 |
NN |
denotes |
dA–dT |
T4997 |
293-294 |
-RRB- |
denotes |
) |
T4998 |
295-305 |
NN |
denotes |
copolymers |
T4999 |
306-307 |
-LRB- |
denotes |
( |
T5000 |
307-315 |
NNP |
denotes |
Amersham |
T5001 |
316-325 |
NNP |
denotes |
Pharmacia |
T5002 |
326-333 |
NNP |
denotes |
Biotech |
T5003 |
333-334 |
-RRB- |
denotes |
) |
T5004 |
335-339 |
VB |
denotes |
were |
T5005 |
340-351 |
VB |
denotes |
denaturated |
T5006 |
352-355 |
IN |
denotes |
for |
T5007 |
356-358 |
CD |
denotes |
20 |
T5008 |
359-362 |
NN |
denotes |
min |
T5009 |
363-365 |
IN |
denotes |
at |
T5010 |
366-370 |
NN |
denotes |
42°C |
T5011 |
371-373 |
IN |
denotes |
in |
T5012 |
374-377 |
CD |
denotes |
0.3 |
T5013 |
378-379 |
NN |
denotes |
M |
T5014 |
380-384 |
NN |
denotes |
NaOH |
T5015 |
385-387 |
IN |
denotes |
in |
T5016 |
388-389 |
DT |
denotes |
a |
T5017 |
390-396 |
NN |
denotes |
volume |
T5018 |
397-399 |
IN |
denotes |
of |
T5019 |
400-402 |
CD |
denotes |
50 |
T5020 |
403-405 |
NN |
denotes |
µl |
T5021 |
407-412 |
JJ |
denotes |
Fresh |
T5022 |
413-422 |
NN |
denotes |
solutions |
T5023 |
423-425 |
IN |
denotes |
of |
T5024 |
426-428 |
CD |
denotes |
30 |
T5025 |
429-431 |
NN |
denotes |
µl |
T5026 |
432-434 |
IN |
denotes |
of |
T5027 |
435-437 |
CD |
denotes |
10 |
T5028 |
438-440 |
NN |
denotes |
mM |
T5029 |
441-452 |
NN |
denotes |
hydrochinon |
T5030 |
453-454 |
-LRB- |
denotes |
( |
T5031 |
454-459 |
NN |
denotes |
Sigma |
T5032 |
459-460 |
-RRB- |
denotes |
) |
T5033 |
461-464 |
CC |
denotes |
and |
T5034 |
465-468 |
CD |
denotes |
530 |
T5035 |
469-471 |
NN |
denotes |
µl |
T5036 |
472-474 |
IN |
denotes |
of |
T5037 |
475-476 |
CD |
denotes |
3 |
T5038 |
477-478 |
NN |
denotes |
M |
T5039 |
479-485 |
NN |
denotes |
sodium |
T5040 |
486-495 |
NN |
denotes |
bisulfite |
T5041 |
496-497 |
-LRB- |
denotes |
( |
T5042 |
497-499 |
NN |
denotes |
pH |
T5043 |
500-503 |
CD |
denotes |
5.0 |
T5044 |
503-504 |
-COLON- |
denotes |
; |
T5045 |
505-510 |
NNP |
denotes |
Sigma |
T5046 |
510-511 |
-RRB- |
denotes |
) |
T5047 |
512-516 |
VB |
denotes |
were |
T5048 |
517-522 |
VB |
denotes |
added |
T5049 |
522-523 |
-COMMA- |
denotes |
, |
T5050 |
524-527 |
DT |
denotes |
the |
T5051 |
528-536 |
NN |
denotes |
solution |
T5052 |
537-540 |
VB |
denotes |
was |
T5053 |
541-547 |
RB |
denotes |
gently |
T5054 |
548-553 |
VB |
denotes |
mixed |
T5055 |
553-554 |
-COMMA- |
denotes |
, |
T5056 |
555-564 |
VB |
denotes |
overlayed |
T5057 |
565-569 |
IN |
denotes |
with |
T5058 |
570-577 |
NN |
denotes |
mineral |
T5059 |
578-581 |
NN |
denotes |
oil |
T5060 |
582-585 |
CC |
denotes |
and |
T5061 |
586-595 |
VB |
denotes |
incubated |
T5062 |
596-598 |
IN |
denotes |
in |
T5063 |
599-602 |
DT |
denotes |
the |
T5064 |
603-607 |
NN |
denotes |
dark |
T5065 |
608-611 |
IN |
denotes |
for |
T5066 |
612-617 |
CD |
denotes |
12–13 |
T5067 |
618-619 |
NN |
denotes |
h |
T5068 |
620-622 |
IN |
denotes |
at |
T5069 |
623-628 |
NNP |
denotes |
50°C. |
T5070 |
629-632 |
DT |
denotes |
The |
T5071 |
633-640 |
JJ |
denotes |
aqueous |
T5072 |
641-646 |
NN |
denotes |
phase |
T5073 |
647-650 |
VB |
denotes |
was |
T5074 |
651-660 |
VB |
denotes |
recovered |
T5075 |
661-666 |
VB |
denotes |
using |
T5076 |
667-670 |
DT |
denotes |
the |
T5077 |
671-678 |
NNP |
denotes |
‘Wizard |
T5078 |
679-682 |
NN |
denotes |
DNA |
T5079 |
683-691 |
NN |
denotes |
clean-up |
T5080 |
692-699 |
NN |
denotes |
system’ |
T5081 |
700-701 |
-LRB- |
denotes |
( |
T5082 |
701-708 |
NNP |
denotes |
Promega |
T5083 |
708-709 |
-COMMA- |
denotes |
, |
T5084 |
710-718 |
NNP |
denotes |
Mannheim |
T5085 |
718-719 |
-COMMA- |
denotes |
, |
T5086 |
720-727 |
NNP |
denotes |
Germany |
T5087 |
727-728 |
-RRB- |
denotes |
) |
T5088 |
730-733 |
DT |
denotes |
The |
T5089 |
734-742 |
VB |
denotes |
purified |
T5090 |
743-746 |
NN |
denotes |
DNA |
T5091 |
747-750 |
VB |
denotes |
was |
T5092 |
751-763 |
RB |
denotes |
subsequently |
T5093 |
764-769 |
VB |
denotes |
mixed |
T5094 |
770-774 |
IN |
denotes |
with |
T5095 |
775-776 |
CD |
denotes |
1 |
T5096 |
777-778 |
NN |
denotes |
M |
T5097 |
779-783 |
NN |
denotes |
NaOH |
T5098 |
784-786 |
TO |
denotes |
to |
T5099 |
787-788 |
DT |
denotes |
a |
T5100 |
789-794 |
JJ |
denotes |
final |
T5101 |
795-808 |
NN |
denotes |
concentration |
T5102 |
809-811 |
IN |
denotes |
of |
T5103 |
812-815 |
CD |
denotes |
0.3 |
T5104 |
816-817 |
NN |
denotes |
M |
T5105 |
818-821 |
CC |
denotes |
and |
T5106 |
822-831 |
VB |
denotes |
incubated |
T5107 |
832-835 |
IN |
denotes |
for |
T5108 |
836-838 |
CD |
denotes |
20 |
T5109 |
839-842 |
NN |
denotes |
min |
T5110 |
843-845 |
IN |
denotes |
at |
T5111 |
846-850 |
NN |
denotes |
37°C |
T5112 |
851-853 |
TO |
denotes |
to |
T5113 |
854-860 |
VB |
denotes |
ensure |
T5114 |
861-869 |
JJ |
denotes |
complete |
T5115 |
870-885 |
NN |
denotes |
desulfonisation |
T5116 |
887-890 |
NN |
denotes |
DNA |
T5117 |
891-894 |
VB |
denotes |
was |
T5118 |
895-902 |
NN |
denotes |
ethanol |
T5119 |
903-915 |
VB |
denotes |
precipitated |
T5120 |
916-918 |
IN |
denotes |
in |
T5121 |
919-922 |
DT |
denotes |
the |
T5122 |
923-931 |
NN |
denotes |
presence |
T5123 |
932-934 |
IN |
denotes |
of |
T5124 |
935-939 |
CD |
denotes |
1/10 |
T5125 |
940-943 |
NN |
denotes |
vol |
T5126 |
944-946 |
IN |
denotes |
of |
T5127 |
947-948 |
CD |
denotes |
3 |
T5128 |
949-950 |
NN |
denotes |
M |
T5129 |
951-957 |
NN |
denotes |
sodium |
T5130 |
958-965 |
NN |
denotes |
acetate |
T5131 |
965-966 |
-COMMA- |
denotes |
, |
T5132 |
967-973 |
VB |
denotes |
washed |
T5133 |
974-978 |
IN |
denotes |
with |
T5134 |
979-981 |
CD |
denotes |
70 |
T5135 |
981-982 |
NN |
denotes |
% |
T5136 |
983-990 |
NN |
denotes |
ethanol |
T5137 |
991-994 |
CC |
denotes |
and |
T5138 |
995-1006 |
VB |
denotes |
resuspended |
T5139 |
1007-1009 |
IN |
denotes |
in |
T5140 |
1010-1012 |
CD |
denotes |
50 |
T5141 |
1013-1015 |
NN |
denotes |
µl |
T5142 |
1016-1019 |
NN |
denotes |
H2O |
T5143 |
1021-1031 |
JJ |
denotes |
Subsequent |
T5144 |
1032-1035 |
NN |
denotes |
PCR |
T5145 |
1036-1049 |
NN |
denotes |
amplification |
T5146 |
1050-1052 |
IN |
denotes |
of |
T5147 |
1053-1054 |
CD |
denotes |
4 |
T5148 |
1055-1057 |
NN |
denotes |
µl |
T5149 |
1058-1075 |
JJ |
denotes |
bisulfite-treated |
T5150 |
1076-1079 |
NN |
denotes |
DNA |
T5151 |
1080-1083 |
VB |
denotes |
was |
T5152 |
1084-1088 |
VB |
denotes |
used |
T5153 |
1089-1092 |
IN |
denotes |
for |
T5154 |
1093-1100 |
NN |
denotes |
cloning |
T5155 |
1101-1103 |
IN |
denotes |
of |
T5156 |
1104-1107 |
CD |
denotes |
two |
T5157 |
1108-1117 |
NN |
denotes |
fragments |
T5158 |
1118-1120 |
IN |
denotes |
of |
T5159 |
1121-1124 |
DT |
denotes |
the |
T5160 |
1125-1130 |
NN |
denotes |
IRF-4 |
T5161 |
1131-1139 |
NN |
denotes |
promoter |
T5162 |
1140-1141 |
-LRB- |
denotes |
( |
T5163 |
1141-1145 |
NN |
denotes |
BS-I |
T5164 |
1146-1149 |
CC |
denotes |
and |
T5165 |
1150-1155 |
NN |
denotes |
BS-II |
T5166 |
1155-1156 |
-RRB- |
denotes |
) |
T5167 |
1157-1161 |
IN |
denotes |
into |
T5168 |
1162-1168 |
NN |
denotes |
pCR2.1 |
T5169 |
1169-1175 |
NN |
denotes |
vector |
T5170 |
1176-1180 |
IN |
denotes |
with |
T5171 |
1181-1184 |
DT |
denotes |
the |
T5172 |
1185-1190 |
NN |
denotes |
‘TOPO |
T5173 |
1191-1193 |
NN |
denotes |
TA |
T5174 |
1194-1201 |
NN |
denotes |
cloning |
T5175 |
1202-1206 |
NN |
denotes |
kit’ |
T5176 |
1207-1208 |
-LRB- |
denotes |
( |
T5177 |
1208-1218 |
NN |
denotes |
Invitrogen |
T5178 |
1218-1219 |
-RRB- |
denotes |
) |
T5179 |
1220-1221 |
-LRB- |
denotes |
( |
T5180 |
1221-1224 |
VB |
denotes |
see |
T5181 |
1225-1231 |
NN |
denotes |
Figure |
T5182 |
1232-1234 |
NN |
denotes |
3A |
T5183 |
1234-1235 |
-RRB- |
denotes |
) |
T5184 |
1237-1240 |
DT |
denotes |
The |
T5185 |
1241-1248 |
NN |
denotes |
primers |
T5186 |
1249-1253 |
VB |
denotes |
used |
T5187 |
1254-1257 |
IN |
denotes |
for |
T5188 |
1258-1261 |
NN |
denotes |
PCR |
T5189 |
1262-1275 |
NN |
denotes |
amplification |
T5190 |
1276-1278 |
IN |
denotes |
of |
T5191 |
1279-1282 |
DT |
denotes |
the |
T5192 |
1283-1287 |
NN |
denotes |
BS-I |
T5193 |
1288-1291 |
CC |
denotes |
and |
T5194 |
1292-1297 |
NN |
denotes |
BS-II |
T5195 |
1298-1307 |
NN |
denotes |
fragments |
T5196 |
1308-1315 |
VB |
denotes |
contain |
T5197 |
1316-1319 |
DT |
denotes |
the |
T5198 |
1320-1328 |
JJ |
denotes |
putative |
T5199 |
1329-1336 |
JJ |
denotes |
altered |
T5200 |
1337-1345 |
NN |
denotes |
sequence |
T5201 |
1346-1348 |
IN |
denotes |
of |
T5202 |
1349-1352 |
DT |
denotes |
the |
T5203 |
1353-1358 |
NN |
denotes |
sense |
T5204 |
1359-1365 |
NN |
denotes |
strand |
T5205 |
1366-1369 |
JJ |
denotes |
due |
T5206 |
1370-1372 |
TO |
denotes |
to |
T5207 |
1373-1382 |
VB |
denotes |
bisulfite |
T5208 |
1383-1392 |
NN |
denotes |
treatment |
T5209 |
1393-1394 |
-LRB- |
denotes |
( |
T5210 |
1394-1403 |
VB |
denotes |
converted |
T5211 |
1404-1412 |
NN |
denotes |
cytosine |
T5212 |
1413-1421 |
NN |
denotes |
residues |
T5213 |
1422-1425 |
VB |
denotes |
are |
T5214 |
1426-1433 |
VB |
denotes |
written |
T5215 |
1434-1436 |
IN |
denotes |
in |
T5216 |
1437-1441 |
JJ |
denotes |
bold |
T5217 |
1442-1449 |
NN |
denotes |
letters |
T5218 |
1449-1450 |
-RRB- |
denotes |
) |
T5219 |
1450-1451 |
-COLON- |
denotes |
: |
T5220 |
1452-1464 |
JJ |
denotes |
BS-I-forward |
T5221 |
1465-1498 |
NN |
denotes |
5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′ |
T5222 |
1498-1499 |
-COMMA- |
denotes |
, |
T5223 |
1500-1512 |
JJ |
denotes |
BS-I-reverse |
T5224 |
1513-1545 |
NN |
denotes |
5′-ACCCAACTCCCTTAAACTATTAAACT-3′ |
T5225 |
1546-1547 |
-LRB- |
denotes |
( |
T5226 |
1547-1550 |
CD |
denotes |
187 |
T5227 |
1551-1553 |
NN |
denotes |
bp |
T5228 |
1553-1554 |
-RRB- |
denotes |
) |
T5229 |
1554-1555 |
-COLON- |
denotes |
; |
T5230 |
1556-1569 |
JJ |
denotes |
BS-II-forward |
T5231 |
1570-1602 |
NN |
denotes |
5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′ |
T5232 |
1602-1603 |
-COMMA- |
denotes |
, |
T5233 |
1604-1617 |
JJ |
denotes |
BS-II-reverse |
T5234 |
1618-1650 |
NN |
denotes |
5′-CTCACCCTAAACTCAAAACTAAAAAC-3′ |
T5235 |
1651-1652 |
-LRB- |
denotes |
( |
T5236 |
1652-1655 |
CD |
denotes |
674 |
T5237 |
1656-1658 |
NN |
denotes |
bp |
T5238 |
1658-1659 |
-RRB- |
denotes |
) |
T5239 |
1661-1666 |
IN |
denotes |
After |
T5240 |
1667-1676 |
JJ |
denotes |
bacterial |
T5241 |
1677-1690 |
NN |
denotes |
amplification |
T5242 |
1691-1693 |
IN |
denotes |
of |
T5243 |
1694-1697 |
DT |
denotes |
the |
T5244 |
1698-1704 |
VB |
denotes |
cloned |
T5245 |
1705-1708 |
NN |
denotes |
PCR |
T5246 |
1709-1718 |
NN |
denotes |
fragments |
T5247 |
1719-1721 |
IN |
denotes |
by |
T5248 |
1722-1730 |
JJ |
denotes |
standard |
T5249 |
1731-1741 |
NN |
denotes |
procedures |
T5250 |
1741-1742 |
-COMMA- |
denotes |
, |
T5251 |
1743-1748 |
CD |
denotes |
eight |
T5252 |
1749-1755 |
NN |
denotes |
clones |
T5253 |
1756-1760 |
IN |
denotes |
from |
T5254 |
1761-1765 |
DT |
denotes |
each |
T5255 |
1766-1772 |
NN |
denotes |
sample |
T5256 |
1773-1777 |
VB |
denotes |
were |
T5257 |
1778-1787 |
VB |
denotes |
sequenced |
T5258 |
1788-1792 |
IN |
denotes |
with |
T5259 |
1793-1795 |
DT |
denotes |
an |
T5260 |
1796-1805 |
VB |
denotes |
automated |
T5261 |
1806-1815 |
NN |
denotes |
sequencer |
T5262 |
1816-1817 |
-LRB- |
denotes |
( |
T5263 |
1817-1820 |
NNP |
denotes |
ABI |
T5264 |
1821-1826 |
NNP |
denotes |
Prism |
T5265 |
1827-1830 |
CD |
denotes |
377 |
T5266 |
1830-1831 |
-COMMA- |
denotes |
, |
T5267 |
1832-1839 |
NNP |
denotes |
Applied |
T5268 |
1840-1850 |
NNP |
denotes |
Biosystems |
T5269 |
1850-1851 |
-RRB- |
denotes |
) |
R4058 |
T4944 |
T4943 |
arg1Of |
treatment,Bisulfite |
R4059 |
T4945 |
T4946 |
arg1Of |
DNA,was |
R4060 |
T4945 |
T4947 |
arg2Of |
DNA,extracted |
R4061 |
T4947 |
T4946 |
arg2Of |
extracted,was |
R4062 |
T4947 |
T4948 |
arg1Of |
extracted,as |
R4063 |
T4949 |
T4948 |
arg2Of |
described,as |
R4064 |
T4949 |
T4950 |
arg1Of |
described,above |
R4065 |
T4952 |
T4951 |
arg1Of |
treatment,Bisulfite |
R4066 |
T4952 |
T4953 |
arg1Of |
treatment,of |
R4067 |
T4952 |
T4955 |
arg1Of |
treatment,"," |
R4068 |
T4952 |
T4956 |
arg1Of |
treatment,leading |
R4069 |
T4952 |
T4962 |
modOf |
treatment,to |
R4070 |
T4952 |
T4978 |
arg1Of |
treatment,follows |
R4071 |
T4954 |
T4953 |
arg2Of |
DNA,of |
R4072 |
T4956 |
T4957 |
arg1Of |
leading,to |
R4073 |
T4958 |
T4957 |
arg2Of |
conversion,to |
R4074 |
T4958 |
T4959 |
arg1Of |
conversion,of |
R4075 |
T4961 |
T4959 |
arg2Of |
cytosine,of |
R4076 |
T4961 |
T4960 |
arg1Of |
cytosine,unmethylated |
R4077 |
T4963 |
T4972 |
arg1Of |
uracil,"," |
R4078 |
T4964 |
T4965 |
arg1Of |
residues,and |
R4079 |
T4965 |
T4963 |
arg2Of |
and,uracil |
R4080 |
T4967 |
T4965 |
arg2Of |
change,and |
R4081 |
T4967 |
T4966 |
arg1Of |
change,no |
R4082 |
T4967 |
T4968 |
arg1Of |
change,of |
R4083 |
T4971 |
T4968 |
arg2Of |
residues,of |
R4084 |
T4971 |
T4969 |
arg2Of |
residues,methylated |
R4085 |
T4971 |
T4970 |
arg1Of |
residues,cytosine |
R4086 |
T4972 |
T4962 |
arg1Of |
",",to |
R4087 |
T4974 |
T4972 |
arg2Of |
performed,"," |
R4088 |
T4974 |
T4973 |
arg2Of |
performed,was |
R4089 |
T4974 |
T4975 |
arg1Of |
performed,as |
R4090 |
T4976 |
T4975 |
arg2Of |
described,as |
R4091 |
T4976 |
T4977 |
arg1Of |
described,as |
R4092 |
T4982 |
T4981 |
arg1Of |
µg,1 |
R4093 |
T4982 |
T4983 |
arg1Of |
µg,of |
R4094 |
T4982 |
T4988 |
arg1Of |
µg,of |
R4095 |
T4982 |
T5004 |
arg1Of |
µg,were |
R4096 |
T4982 |
T5005 |
arg2Of |
µg,denaturated |
R4097 |
T4984 |
T4985 |
arg1Of |
DNA,and |
R4098 |
T4985 |
T4983 |
arg2Of |
and,of |
R4099 |
T4987 |
T4985 |
arg2Of |
µg,and |
R4100 |
T4987 |
T4986 |
arg1Of |
µg,2 |
R4101 |
T4989 |
T4990 |
arg1Of |
poly,( |
R4102 |
T4991 |
T4990 |
arg2Of |
dA–dT,( |
R4103 |
T4992 |
T4990 |
arg3Of |
),( |
R4104 |
T4996 |
T4995 |
arg2Of |
dA–dT,( |
R4105 |
T4997 |
T4995 |
arg3Of |
),( |
R4106 |
T4998 |
T4988 |
arg2Of |
copolymers,of |
R4107 |
T4998 |
T4989 |
arg1Of |
copolymers,poly |
R4108 |
T4998 |
T4993 |
arg1Of |
copolymers,( |
R4109 |
T4998 |
T4994 |
arg1Of |
copolymers,poly |
R4110 |
T4998 |
T4995 |
arg1Of |
copolymers,( |
R4111 |
T4998 |
T4999 |
arg1Of |
copolymers,( |
R4112 |
T5002 |
T4999 |
arg2Of |
Biotech,( |
R4113 |
T5002 |
T5000 |
arg1Of |
Biotech,Amersham |
R4114 |
T5002 |
T5001 |
arg1Of |
Biotech,Pharmacia |
R4115 |
T5003 |
T4999 |
arg3Of |
),( |
R4116 |
T5005 |
T4979 |
arg1Of |
denaturated,Briefly |
R4117 |
T5005 |
T4980 |
arg1Of |
denaturated,"," |
R4118 |
T5005 |
T5004 |
arg2Of |
denaturated,were |
R4119 |
T5005 |
T5006 |
arg1Of |
denaturated,for |
R4120 |
T5005 |
T5009 |
arg1Of |
denaturated,at |
R4121 |
T5005 |
T5011 |
arg1Of |
denaturated,in |
R4122 |
T5005 |
T5015 |
arg1Of |
denaturated,in |
R4123 |
T5008 |
T5006 |
arg2Of |
min,for |
R4124 |
T5008 |
T5007 |
arg1Of |
min,20 |
R4125 |
T5010 |
T5009 |
arg2Of |
42°C,at |
R4126 |
T5012 |
T5013 |
arg1Of |
0.3,M |
R4127 |
T5014 |
T5011 |
arg2Of |
NaOH,in |
R4128 |
T5014 |
T5012 |
arg1Of |
NaOH,0.3 |
R4129 |
T5017 |
T5015 |
arg2Of |
volume,in |
R4130 |
T5017 |
T5016 |
arg1Of |
volume,a |
R4131 |
T5017 |
T5018 |
arg1Of |
volume,of |
R4132 |
T5020 |
T5018 |
arg2Of |
µl,of |
R4133 |
T5020 |
T5019 |
arg1Of |
µl,50 |
R4134 |
T5022 |
T5021 |
arg1Of |
solutions,Fresh |
R4135 |
T5022 |
T5023 |
arg1Of |
solutions,of |
R4136 |
T5022 |
T5047 |
arg1Of |
solutions,were |
R4137 |
T5022 |
T5048 |
arg2Of |
solutions,added |
R4138 |
T5025 |
T5024 |
arg1Of |
µl,30 |
R4139 |
T5025 |
T5026 |
arg1Of |
µl,of |
R4140 |
T5025 |
T5033 |
arg1Of |
µl,and |
R4141 |
T5027 |
T5028 |
arg1Of |
10,mM |
R4142 |
T5029 |
T5026 |
arg2Of |
hydrochinon,of |
R4143 |
T5029 |
T5027 |
arg1Of |
hydrochinon,10 |
R4144 |
T5029 |
T5030 |
arg1Of |
hydrochinon,( |
R4145 |
T5031 |
T5030 |
arg2Of |
Sigma,( |
R4146 |
T5032 |
T5030 |
arg3Of |
),( |
R4147 |
T5033 |
T5023 |
arg2Of |
and,of |
R4148 |
T5035 |
T5033 |
arg2Of |
µl,and |
R4149 |
T5035 |
T5034 |
arg1Of |
µl,530 |
R4150 |
T5035 |
T5036 |
arg1Of |
µl,of |
R4151 |
T5037 |
T5038 |
arg1Of |
3,M |
R4152 |
T5040 |
T5036 |
arg2Of |
bisulfite,of |
R4153 |
T5040 |
T5037 |
arg1Of |
bisulfite,3 |
R4154 |
T5040 |
T5039 |
arg1Of |
bisulfite,sodium |
R4155 |
T5040 |
T5041 |
arg1Of |
bisulfite,( |
R4156 |
T5042 |
T5041 |
arg2Of |
pH,( |
R4157 |
T5042 |
T5043 |
arg1Of |
pH,5.0 |
R4158 |
T5042 |
T5044 |
arg1Of |
pH,; |
R4159 |
T5045 |
T5044 |
arg2Of |
Sigma,; |
R4160 |
T5046 |
T5041 |
arg3Of |
),( |
R4161 |
T5048 |
T5047 |
arg2Of |
added,were |
R4162 |
T5051 |
T5050 |
arg1Of |
solution,the |
R4163 |
T5051 |
T5052 |
arg1Of |
solution,was |
R4164 |
T5051 |
T5054 |
arg2Of |
solution,mixed |
R4165 |
T5051 |
T5056 |
arg2Of |
solution,overlayed |
R4166 |
T5051 |
T5061 |
arg2Of |
solution,incubated |
R4167 |
T5054 |
T5055 |
arg1Of |
mixed,"," |
R4168 |
T5055 |
T5060 |
arg1Of |
",",and |
R4169 |
T5056 |
T5055 |
arg2Of |
overlayed,"," |
R4170 |
T5056 |
T5057 |
arg1Of |
overlayed,with |
R4171 |
T5059 |
T5057 |
arg2Of |
oil,with |
R4172 |
T5059 |
T5058 |
arg1Of |
oil,mineral |
R4173 |
T5060 |
T5049 |
arg1Of |
and,"," |
R4174 |
T5060 |
T5052 |
arg2Of |
and,was |
R4175 |
T5060 |
T5053 |
arg1Of |
and,gently |
R4176 |
T5061 |
T5060 |
arg2Of |
incubated,and |
R4177 |
T5061 |
T5062 |
arg1Of |
incubated,in |
R4178 |
T5064 |
T5062 |
arg2Of |
dark,in |
R4179 |
T5064 |
T5063 |
arg1Of |
dark,the |
R4180 |
T5067 |
T5066 |
arg1Of |
h,12–13 |
R4181 |
T5067 |
T5068 |
arg1Of |
h,at |
R4182 |
T5067 |
T5073 |
arg1Of |
h,was |
R4183 |
T5067 |
T5074 |
arg2Of |
h,recovered |
R4184 |
T5072 |
T5068 |
arg2Of |
phase,at |
R4185 |
T5072 |
T5069 |
arg1Of |
phase,50°C. |
R4186 |
T5072 |
T5070 |
arg1Of |
phase,The |
R4187 |
T5072 |
T5071 |
arg1Of |
phase,aqueous |
R4188 |
T5074 |
T5073 |
arg2Of |
recovered,was |
R4189 |
T5074 |
T5075 |
modOf |
recovered,using |
R4190 |
T5074 |
T5081 |
arg1Of |
recovered,( |
R4191 |
T5080 |
T5075 |
arg2Of |
system’,using |
R4192 |
T5080 |
T5076 |
arg1Of |
system’,the |
R4193 |
T5080 |
T5077 |
arg1Of |
system’,‘Wizard |
R4194 |
T5080 |
T5078 |
arg1Of |
system’,DNA |
R4195 |
T5080 |
T5079 |
arg1Of |
system’,clean-up |
R4196 |
T5082 |
T5083 |
arg1Of |
Promega,"," |
R4197 |
T5083 |
T5081 |
arg2Of |
",",( |
R4198 |
T5083 |
T5085 |
arg1Of |
",","," |
R4199 |
T5084 |
T5083 |
arg2Of |
Mannheim,"," |
R4200 |
T5086 |
T5085 |
arg2Of |
Germany,"," |
R4201 |
T5087 |
T5081 |
arg3Of |
),( |
R4202 |
T5090 |
T5088 |
arg1Of |
DNA,The |
R4203 |
T5090 |
T5089 |
arg2Of |
DNA,purified |
R4204 |
T5090 |
T5091 |
arg1Of |
DNA,was |
R4205 |
T5090 |
T5093 |
arg2Of |
DNA,mixed |
R4206 |
T5090 |
T5106 |
arg2Of |
DNA,incubated |
R4207 |
T5093 |
T5094 |
arg1Of |
mixed,with |
R4208 |
T5093 |
T5105 |
arg1Of |
mixed,and |
R4209 |
T5095 |
T5096 |
arg1Of |
1,M |
R4210 |
T5097 |
T5094 |
arg2Of |
NaOH,with |
R4211 |
T5097 |
T5095 |
arg1Of |
NaOH,1 |
R4212 |
T5097 |
T5098 |
arg1Of |
NaOH,to |
R4213 |
T5101 |
T5098 |
arg2Of |
concentration,to |
R4214 |
T5101 |
T5099 |
arg1Of |
concentration,a |
R4215 |
T5101 |
T5100 |
arg1Of |
concentration,final |
R4216 |
T5101 |
T5102 |
arg1Of |
concentration,of |
R4217 |
T5104 |
T5102 |
arg2Of |
M,of |
R4218 |
T5104 |
T5103 |
arg1Of |
M,0.3 |
R4219 |
T5105 |
T5091 |
arg2Of |
and,was |
R4220 |
T5105 |
T5092 |
arg1Of |
and,subsequently |
R4221 |
T5106 |
T5105 |
arg2Of |
incubated,and |
R4222 |
T5106 |
T5107 |
arg1Of |
incubated,for |
R4223 |
T5106 |
T5110 |
arg1Of |
incubated,at |
R4224 |
T5106 |
T5112 |
modOf |
incubated,to |
R4225 |
T5109 |
T5107 |
arg2Of |
min,for |
R4226 |
T5109 |
T5108 |
arg1Of |
min,20 |
R4227 |
T5111 |
T5110 |
arg2Of |
37°C,at |
R4228 |
T5113 |
T5112 |
arg1Of |
ensure,to |
R4229 |
T5115 |
T5113 |
arg2Of |
desulfonisation,ensure |
R4230 |
T5115 |
T5114 |
arg1Of |
desulfonisation,complete |
R4231 |
T5116 |
T5117 |
arg1Of |
DNA,was |
R4232 |
T5116 |
T5138 |
arg1Of |
DNA,resuspended |
R4233 |
T5117 |
T5137 |
arg1Of |
was,and |
R4234 |
T5118 |
T5117 |
arg2Of |
ethanol,was |
R4235 |
T5118 |
T5119 |
arg2Of |
ethanol,precipitated |
R4236 |
T5119 |
T5120 |
arg1Of |
precipitated,in |
R4237 |
T5122 |
T5120 |
arg2Of |
presence,in |
R4238 |
T5122 |
T5121 |
arg1Of |
presence,the |
R4239 |
T5122 |
T5123 |
arg1Of |
presence,of |
R4240 |
T5122 |
T5131 |
arg1Of |
presence,"," |
R4241 |
T5122 |
T5132 |
arg2Of |
presence,washed |
R4242 |
T5125 |
T5123 |
arg2Of |
vol,of |
R4243 |
T5125 |
T5124 |
arg1Of |
vol,1/10 |
R4244 |
T5125 |
T5126 |
arg1Of |
vol,of |
R4245 |
T5127 |
T5128 |
arg1Of |
3,M |
R4246 |
T5130 |
T5126 |
arg2Of |
acetate,of |
R4247 |
T5130 |
T5127 |
arg1Of |
acetate,3 |
R4248 |
T5130 |
T5129 |
arg1Of |
acetate,sodium |
R4249 |
T5132 |
T5133 |
arg1Of |
washed,with |
R4250 |
T5134 |
T5135 |
arg1Of |
70,% |
R4251 |
T5136 |
T5133 |
arg2Of |
ethanol,with |
R4252 |
T5136 |
T5134 |
arg1Of |
ethanol,70 |
R4253 |
T5138 |
T5137 |
arg2Of |
resuspended,and |
R4254 |
T5138 |
T5139 |
arg1Of |
resuspended,in |
R4255 |
T5142 |
T5139 |
arg2Of |
H2O,in |
R4256 |
T5142 |
T5140 |
arg1Of |
H2O,50 |
R4257 |
T5142 |
T5141 |
arg1Of |
H2O,µl |
R4258 |
T5145 |
T5143 |
arg1Of |
amplification,Subsequent |
R4259 |
T5145 |
T5144 |
arg1Of |
amplification,PCR |
R4260 |
T5145 |
T5146 |
arg1Of |
amplification,of |
R4261 |
T5145 |
T5151 |
arg1Of |
amplification,was |
R4262 |
T5145 |
T5152 |
arg2Of |
amplification,used |
R4263 |
T5150 |
T5146 |
arg2Of |
DNA,of |
R4264 |
T5150 |
T5147 |
arg1Of |
DNA,4 |
R4265 |
T5150 |
T5148 |
arg1Of |
DNA,µl |
R4266 |
T5150 |
T5149 |
arg1Of |
DNA,bisulfite-treated |
R4267 |
T5152 |
T5151 |
arg2Of |
used,was |
R4268 |
T5152 |
T5153 |
arg1Of |
used,for |
R4269 |
T5152 |
T5167 |
arg1Of |
used,into |
R4270 |
T5152 |
T5179 |
arg1Of |
used,( |
R4271 |
T5154 |
T5153 |
arg2Of |
cloning,for |
R4272 |
T5154 |
T5155 |
arg1Of |
cloning,of |
R4273 |
T5157 |
T5155 |
arg2Of |
fragments,of |
R4274 |
T5157 |
T5156 |
arg1Of |
fragments,two |
R4275 |
T5157 |
T5158 |
arg1Of |
fragments,of |
R4276 |
T5157 |
T5162 |
arg1Of |
fragments,( |
R4277 |
T5161 |
T5158 |
arg2Of |
promoter,of |
R4278 |
T5161 |
T5159 |
arg1Of |
promoter,the |
R4279 |
T5161 |
T5160 |
arg1Of |
promoter,IRF-4 |
R4280 |
T5163 |
T5164 |
arg1Of |
BS-I,and |
R4281 |
T5164 |
T5162 |
arg2Of |
and,( |
R4282 |
T5165 |
T5164 |
arg2Of |
BS-II,and |
R4283 |
T5166 |
T5162 |
arg3Of |
),( |
R4284 |
T5169 |
T5167 |
arg2Of |
vector,into |
R4285 |
T5169 |
T5168 |
arg1Of |
vector,pCR2.1 |
R4286 |
T5169 |
T5170 |
arg1Of |
vector,with |
R4287 |
T5175 |
T5170 |
arg2Of |
kit’,with |
R4288 |
T5175 |
T5171 |
arg1Of |
kit’,the |
R4289 |
T5175 |
T5172 |
arg1Of |
kit’,‘TOPO |
R4290 |
T5175 |
T5173 |
arg1Of |
kit’,TA |
R4291 |
T5175 |
T5174 |
arg1Of |
kit’,cloning |
R4292 |
T5175 |
T5176 |
arg1Of |
kit’,( |
R4293 |
T5177 |
T5176 |
arg2Of |
Invitrogen,( |
R4294 |
T5178 |
T5176 |
arg3Of |
),( |
R4295 |
T5180 |
T5179 |
arg2Of |
see,( |
R4296 |
T5182 |
T5180 |
arg2Of |
3A,see |
R4297 |
T5182 |
T5181 |
arg1Of |
3A,Figure |
R4298 |
T5183 |
T5179 |
arg3Of |
),( |
R4299 |
T5185 |
T5184 |
arg1Of |
primers,The |
R4300 |
T5185 |
T5186 |
arg2Of |
primers,used |
R4301 |
T5185 |
T5196 |
arg1Of |
primers,contain |
R4302 |
T5186 |
T5187 |
arg1Of |
used,for |
R4303 |
T5189 |
T5187 |
arg2Of |
amplification,for |
R4304 |
T5189 |
T5188 |
arg1Of |
amplification,PCR |
R4305 |
T5189 |
T5190 |
arg1Of |
amplification,of |
R4306 |
T5192 |
T5193 |
arg1Of |
BS-I,and |
R4307 |
T5194 |
T5193 |
arg2Of |
BS-II,and |
R4308 |
T5195 |
T5190 |
arg2Of |
fragments,of |
R4309 |
T5195 |
T5191 |
arg1Of |
fragments,the |
R4310 |
T5195 |
T5192 |
arg1Of |
fragments,BS-I |
R4311 |
T5195 |
T5194 |
arg1Of |
fragments,BS-II |
R4312 |
T5196 |
T5219 |
arg1Of |
contain,: |
R4313 |
T5196 |
T5221 |
arg1Of |
contain,5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′ |
R4314 |
T5196 |
T5229 |
arg1Of |
contain,; |
R4315 |
T5196 |
T5231 |
arg1Of |
contain,5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′ |
R4316 |
T5200 |
T5196 |
arg2Of |
sequence,contain |
R4317 |
T5200 |
T5197 |
arg1Of |
sequence,the |
R4318 |
T5200 |
T5198 |
arg1Of |
sequence,putative |
R4319 |
T5200 |
T5199 |
arg1Of |
sequence,altered |
R4320 |
T5200 |
T5201 |
arg1Of |
sequence,of |
R4321 |
T5204 |
T5201 |
arg2Of |
strand,of |
R4322 |
T5204 |
T5202 |
arg1Of |
strand,the |
R4323 |
T5204 |
T5203 |
arg1Of |
strand,sense |
R4324 |
T5204 |
T5205 |
arg1Of |
strand,due |
R4325 |
T5207 |
T5205 |
arg2Of |
bisulfite,due |
R4326 |
T5207 |
T5206 |
arg1Of |
bisulfite,to |
R4327 |
T5208 |
T5207 |
arg2Of |
treatment,bisulfite |
R4328 |
T5208 |
T5209 |
arg1Of |
treatment,( |
R4329 |
T5212 |
T5210 |
arg2Of |
residues,converted |
R4330 |
T5212 |
T5211 |
arg1Of |
residues,cytosine |
R4331 |
T5212 |
T5213 |
arg1Of |
residues,are |
R4332 |
T5212 |
T5214 |
arg2Of |
residues,written |
R4333 |
T5214 |
T5209 |
arg2Of |
written,( |
R4334 |
T5214 |
T5213 |
arg2Of |
written,are |
R4335 |
T5214 |
T5215 |
arg1Of |
written,in |
R4336 |
T5217 |
T5215 |
arg2Of |
letters,in |
R4337 |
T5217 |
T5216 |
arg1Of |
letters,bold |
R4338 |
T5218 |
T5209 |
arg3Of |
),( |
R4339 |
T5221 |
T5220 |
arg1Of |
5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′,BS-I-forward |
R4340 |
T5221 |
T5222 |
arg1Of |
5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′,"," |
R4341 |
T5224 |
T5222 |
arg2Of |
5′-ACCCAACTCCCTTAAACTATTAAACT-3′,"," |
R4342 |
T5224 |
T5223 |
arg1Of |
5′-ACCCAACTCCCTTAAACTATTAAACT-3′,BS-I-reverse |
R4343 |
T5224 |
T5225 |
arg1Of |
5′-ACCCAACTCCCTTAAACTATTAAACT-3′,( |
R4344 |
T5227 |
T5225 |
arg2Of |
bp,( |
R4345 |
T5227 |
T5226 |
arg1Of |
bp,187 |
R4346 |
T5228 |
T5225 |
arg3Of |
),( |
R4347 |
T5231 |
T5230 |
arg1Of |
5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′,BS-II-forward |
R4348 |
T5231 |
T5232 |
arg1Of |
5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′,"," |
R4349 |
T5234 |
T5232 |
arg2Of |
5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,"," |
R4350 |
T5234 |
T5233 |
arg1Of |
5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,BS-II-reverse |
R4351 |
T5234 |
T5235 |
arg1Of |
5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,( |
R4352 |
T5237 |
T5235 |
arg2Of |
bp,( |
R4353 |
T5237 |
T5236 |
arg1Of |
bp,674 |
R4354 |
T5238 |
T5235 |
arg3Of |
),( |
R4355 |
T5241 |
T5239 |
arg2Of |
amplification,After |
R4356 |
T5241 |
T5240 |
arg1Of |
amplification,bacterial |
R4357 |
T5241 |
T5242 |
arg1Of |
amplification,of |
R4358 |
T5241 |
T5247 |
arg1Of |
amplification,by |
R4359 |
T5246 |
T5242 |
arg2Of |
fragments,of |
R4360 |
T5246 |
T5243 |
arg1Of |
fragments,the |
R4361 |
T5246 |
T5244 |
arg2Of |
fragments,cloned |
R4362 |
T5246 |
T5245 |
arg1Of |
fragments,PCR |
R4363 |
T5249 |
T5247 |
arg2Of |
procedures,by |
R4364 |
T5249 |
T5248 |
arg1Of |
procedures,standard |
R4365 |
T5252 |
T5251 |
arg1Of |
clones,eight |
R4366 |
T5252 |
T5253 |
arg1Of |
clones,from |
R4367 |
T5252 |
T5256 |
arg1Of |
clones,were |
R4368 |
T5252 |
T5257 |
arg2Of |
clones,sequenced |
R4369 |
T5255 |
T5253 |
arg2Of |
sample,from |
R4370 |
T5255 |
T5254 |
arg1Of |
sample,each |
R4371 |
T5257 |
T5239 |
arg1Of |
sequenced,After |
R4372 |
T5257 |
T5250 |
arg1Of |
sequenced,"," |
R4373 |
T5257 |
T5256 |
arg2Of |
sequenced,were |
R4374 |
T5257 |
T5258 |
arg1Of |
sequenced,with |
R4375 |
T5257 |
T5262 |
arg1Of |
sequenced,( |
R4376 |
T5261 |
T5258 |
arg2Of |
sequencer,with |
R4377 |
T5261 |
T5259 |
arg1Of |
sequencer,an |
R4378 |
T5261 |
T5260 |
arg2Of |
sequencer,automated |
R4379 |
T5264 |
T5262 |
arg2Of |
Prism,( |
R4380 |
T5264 |
T5263 |
arg1Of |
Prism,ABI |
R4381 |
T5264 |
T5265 |
arg1Of |
Prism,377 |
R4382 |
T5264 |
T5266 |
arg1Of |
Prism,"," |
R4383 |
T5268 |
T5266 |
arg2Of |
Biosystems,"," |
R4384 |
T5268 |
T5267 |
arg1Of |
Biosystems,Applied |
R4385 |
T5269 |
T5262 |
arg3Of |
),( |