PMC:1310901 / 10203-12055 JSONTXT 14 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4943 0-9 NN denotes Bisulfite
T4944 10-19 NN denotes treatment
T4945 20-23 NN denotes DNA
T4946 24-27 VB denotes was
T4947 28-37 VB denotes extracted
T4948 38-40 IN denotes as
T4949 41-50 VB denotes described
T4950 51-56 RB denotes above
T4951 58-67 NN denotes Bisulfite
T4952 68-77 NN denotes treatment
T4953 78-80 IN denotes of
T4954 81-84 NN denotes DNA
T4955 84-85 -COMMA- denotes ,
T4956 86-93 VB denotes leading
T4957 94-96 TO denotes to
T4958 97-107 NN denotes conversion
T4959 108-110 IN denotes of
T4960 111-123 JJ denotes unmethylated
T4961 124-132 NN denotes cytosine
T4962 133-135 TO denotes to
T4963 136-142 NN denotes uracil
T4964 143-151 NN denotes residues
T4965 152-155 CC denotes and
T4966 156-158 DT denotes no
T4967 159-165 NN denotes change
T4968 166-168 IN denotes of
T4969 169-179 VB denotes methylated
T4970 180-188 NN denotes cytosine
T4971 189-197 NN denotes residues
T4972 197-198 -COMMA- denotes ,
T4973 199-202 VB denotes was
T4974 203-212 VB denotes performed
T4975 213-215 IN denotes as
T4976 216-225 VB denotes described
T4977 226-228 IN denotes as
T4978 229-236 VB denotes follows
T4979 238-245 RB denotes Briefly
T4980 245-246 -COMMA- denotes ,
T4981 247-248 CD denotes 1
T4982 249-251 NN denotes µg
T4983 252-254 IN denotes of
T4984 255-258 NN denotes DNA
T4985 259-262 CC denotes and
T4986 263-264 CD denotes 2
T4987 265-267 NN denotes µg
T4988 268-270 IN denotes of
T4989 271-275 NN denotes poly
T4990 275-276 -LRB- denotes (
T4991 276-281 NN denotes dA–dT
T4992 281-282 -RRB- denotes )
T4993 282-283 -LRB- denotes (
T4994 283-287 NN denotes poly
T4995 287-288 -LRB- denotes (
T4996 288-293 NN denotes dA–dT
T4997 293-294 -RRB- denotes )
T4998 295-305 NN denotes copolymers
T4999 306-307 -LRB- denotes (
T5000 307-315 NNP denotes Amersham
T5001 316-325 NNP denotes Pharmacia
T5002 326-333 NNP denotes Biotech
T5003 333-334 -RRB- denotes )
T5004 335-339 VB denotes were
T5005 340-351 VB denotes denaturated
T5006 352-355 IN denotes for
T5007 356-358 CD denotes 20
T5008 359-362 NN denotes min
T5009 363-365 IN denotes at
T5010 366-370 NN denotes 42°C
T5011 371-373 IN denotes in
T5012 374-377 CD denotes 0.3
T5013 378-379 NN denotes M
T5014 380-384 NN denotes NaOH
T5015 385-387 IN denotes in
T5016 388-389 DT denotes a
T5017 390-396 NN denotes volume
T5018 397-399 IN denotes of
T5019 400-402 CD denotes 50
T5020 403-405 NN denotes µl
T5021 407-412 JJ denotes Fresh
T5022 413-422 NN denotes solutions
T5023 423-425 IN denotes of
T5024 426-428 CD denotes 30
T5025 429-431 NN denotes µl
T5026 432-434 IN denotes of
T5027 435-437 CD denotes 10
T5028 438-440 NN denotes mM
T5029 441-452 NN denotes hydrochinon
T5030 453-454 -LRB- denotes (
T5031 454-459 NN denotes Sigma
T5032 459-460 -RRB- denotes )
T5033 461-464 CC denotes and
T5034 465-468 CD denotes 530
T5035 469-471 NN denotes µl
T5036 472-474 IN denotes of
T5037 475-476 CD denotes 3
T5038 477-478 NN denotes M
T5039 479-485 NN denotes sodium
T5040 486-495 NN denotes bisulfite
T5041 496-497 -LRB- denotes (
T5042 497-499 NN denotes pH
T5043 500-503 CD denotes 5.0
T5044 503-504 -COLON- denotes ;
T5045 505-510 NNP denotes Sigma
T5046 510-511 -RRB- denotes )
T5047 512-516 VB denotes were
T5048 517-522 VB denotes added
T5049 522-523 -COMMA- denotes ,
T5050 524-527 DT denotes the
T5051 528-536 NN denotes solution
T5052 537-540 VB denotes was
T5053 541-547 RB denotes gently
T5054 548-553 VB denotes mixed
T5055 553-554 -COMMA- denotes ,
T5056 555-564 VB denotes overlayed
T5057 565-569 IN denotes with
T5058 570-577 NN denotes mineral
T5059 578-581 NN denotes oil
T5060 582-585 CC denotes and
T5061 586-595 VB denotes incubated
T5062 596-598 IN denotes in
T5063 599-602 DT denotes the
T5064 603-607 NN denotes dark
T5065 608-611 IN denotes for
T5066 612-617 CD denotes 12–13
T5067 618-619 NN denotes h
T5068 620-622 IN denotes at
T5069 623-628 NNP denotes 50°C.
T5070 629-632 DT denotes The
T5071 633-640 JJ denotes aqueous
T5072 641-646 NN denotes phase
T5073 647-650 VB denotes was
T5074 651-660 VB denotes recovered
T5075 661-666 VB denotes using
T5076 667-670 DT denotes the
T5077 671-678 NNP denotes ‘Wizard
T5078 679-682 NN denotes DNA
T5079 683-691 NN denotes clean-up
T5080 692-699 NN denotes system’
T5081 700-701 -LRB- denotes (
T5082 701-708 NNP denotes Promega
T5083 708-709 -COMMA- denotes ,
T5084 710-718 NNP denotes Mannheim
T5085 718-719 -COMMA- denotes ,
T5086 720-727 NNP denotes Germany
T5087 727-728 -RRB- denotes )
T5088 730-733 DT denotes The
T5089 734-742 VB denotes purified
T5090 743-746 NN denotes DNA
T5091 747-750 VB denotes was
T5092 751-763 RB denotes subsequently
T5093 764-769 VB denotes mixed
T5094 770-774 IN denotes with
T5095 775-776 CD denotes 1
T5096 777-778 NN denotes M
T5097 779-783 NN denotes NaOH
T5098 784-786 TO denotes to
T5099 787-788 DT denotes a
T5100 789-794 JJ denotes final
T5101 795-808 NN denotes concentration
T5102 809-811 IN denotes of
T5103 812-815 CD denotes 0.3
T5104 816-817 NN denotes M
T5105 818-821 CC denotes and
T5106 822-831 VB denotes incubated
T5107 832-835 IN denotes for
T5108 836-838 CD denotes 20
T5109 839-842 NN denotes min
T5110 843-845 IN denotes at
T5111 846-850 NN denotes 37°C
T5112 851-853 TO denotes to
T5113 854-860 VB denotes ensure
T5114 861-869 JJ denotes complete
T5115 870-885 NN denotes desulfonisation
T5116 887-890 NN denotes DNA
T5117 891-894 VB denotes was
T5118 895-902 NN denotes ethanol
T5119 903-915 VB denotes precipitated
T5120 916-918 IN denotes in
T5121 919-922 DT denotes the
T5122 923-931 NN denotes presence
T5123 932-934 IN denotes of
T5124 935-939 CD denotes 1/10
T5125 940-943 NN denotes vol
T5126 944-946 IN denotes of
T5127 947-948 CD denotes 3
T5128 949-950 NN denotes M
T5129 951-957 NN denotes sodium
T5130 958-965 NN denotes acetate
T5131 965-966 -COMMA- denotes ,
T5132 967-973 VB denotes washed
T5133 974-978 IN denotes with
T5134 979-981 CD denotes 70
T5135 981-982 NN denotes %
T5136 983-990 NN denotes ethanol
T5137 991-994 CC denotes and
T5138 995-1006 VB denotes resuspended
T5139 1007-1009 IN denotes in
T5140 1010-1012 CD denotes 50
T5141 1013-1015 NN denotes µl
T5142 1016-1019 NN denotes H2O
T5143 1021-1031 JJ denotes Subsequent
T5144 1032-1035 NN denotes PCR
T5145 1036-1049 NN denotes amplification
T5146 1050-1052 IN denotes of
T5147 1053-1054 CD denotes 4
T5148 1055-1057 NN denotes µl
T5149 1058-1075 JJ denotes bisulfite-treated
T5150 1076-1079 NN denotes DNA
T5151 1080-1083 VB denotes was
T5152 1084-1088 VB denotes used
T5153 1089-1092 IN denotes for
T5154 1093-1100 NN denotes cloning
T5155 1101-1103 IN denotes of
T5156 1104-1107 CD denotes two
T5157 1108-1117 NN denotes fragments
T5158 1118-1120 IN denotes of
T5159 1121-1124 DT denotes the
T5160 1125-1130 NN denotes IRF-4
T5161 1131-1139 NN denotes promoter
T5162 1140-1141 -LRB- denotes (
T5163 1141-1145 NN denotes BS-I
T5164 1146-1149 CC denotes and
T5165 1150-1155 NN denotes BS-II
T5166 1155-1156 -RRB- denotes )
T5167 1157-1161 IN denotes into
T5168 1162-1168 NN denotes pCR2.1
T5169 1169-1175 NN denotes vector
T5170 1176-1180 IN denotes with
T5171 1181-1184 DT denotes the
T5172 1185-1190 NN denotes ‘TOPO
T5173 1191-1193 NN denotes TA
T5174 1194-1201 NN denotes cloning
T5175 1202-1206 NN denotes kit’
T5176 1207-1208 -LRB- denotes (
T5177 1208-1218 NN denotes Invitrogen
T5178 1218-1219 -RRB- denotes )
T5179 1220-1221 -LRB- denotes (
T5180 1221-1224 VB denotes see
T5181 1225-1231 NN denotes Figure
T5182 1232-1234 NN denotes 3A
T5183 1234-1235 -RRB- denotes )
T5184 1237-1240 DT denotes The
T5185 1241-1248 NN denotes primers
T5186 1249-1253 VB denotes used
T5187 1254-1257 IN denotes for
T5188 1258-1261 NN denotes PCR
T5189 1262-1275 NN denotes amplification
T5190 1276-1278 IN denotes of
T5191 1279-1282 DT denotes the
T5192 1283-1287 NN denotes BS-I
T5193 1288-1291 CC denotes and
T5194 1292-1297 NN denotes BS-II
T5195 1298-1307 NN denotes fragments
T5196 1308-1315 VB denotes contain
T5197 1316-1319 DT denotes the
T5198 1320-1328 JJ denotes putative
T5199 1329-1336 JJ denotes altered
T5200 1337-1345 NN denotes sequence
T5201 1346-1348 IN denotes of
T5202 1349-1352 DT denotes the
T5203 1353-1358 NN denotes sense
T5204 1359-1365 NN denotes strand
T5205 1366-1369 JJ denotes due
T5206 1370-1372 TO denotes to
T5207 1373-1382 VB denotes bisulfite
T5208 1383-1392 NN denotes treatment
T5209 1393-1394 -LRB- denotes (
T5210 1394-1403 VB denotes converted
T5211 1404-1412 NN denotes cytosine
T5212 1413-1421 NN denotes residues
T5213 1422-1425 VB denotes are
T5214 1426-1433 VB denotes written
T5215 1434-1436 IN denotes in
T5216 1437-1441 JJ denotes bold
T5217 1442-1449 NN denotes letters
T5218 1449-1450 -RRB- denotes )
T5219 1450-1451 -COLON- denotes :
T5220 1452-1464 JJ denotes BS-I-forward
T5221 1465-1498 NN denotes 5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′
T5222 1498-1499 -COMMA- denotes ,
T5223 1500-1512 JJ denotes BS-I-reverse
T5224 1513-1545 NN denotes 5′-ACCCAACTCCCTTAAACTATTAAACT-3′
T5225 1546-1547 -LRB- denotes (
T5226 1547-1550 CD denotes 187
T5227 1551-1553 NN denotes bp
T5228 1553-1554 -RRB- denotes )
T5229 1554-1555 -COLON- denotes ;
T5230 1556-1569 JJ denotes BS-II-forward
T5231 1570-1602 NN denotes 5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′
T5232 1602-1603 -COMMA- denotes ,
T5233 1604-1617 JJ denotes BS-II-reverse
T5234 1618-1650 NN denotes 5′-CTCACCCTAAACTCAAAACTAAAAAC-3′
T5235 1651-1652 -LRB- denotes (
T5236 1652-1655 CD denotes 674
T5237 1656-1658 NN denotes bp
T5238 1658-1659 -RRB- denotes )
T5239 1661-1666 IN denotes After
T5240 1667-1676 JJ denotes bacterial
T5241 1677-1690 NN denotes amplification
T5242 1691-1693 IN denotes of
T5243 1694-1697 DT denotes the
T5244 1698-1704 VB denotes cloned
T5245 1705-1708 NN denotes PCR
T5246 1709-1718 NN denotes fragments
T5247 1719-1721 IN denotes by
T5248 1722-1730 JJ denotes standard
T5249 1731-1741 NN denotes procedures
T5250 1741-1742 -COMMA- denotes ,
T5251 1743-1748 CD denotes eight
T5252 1749-1755 NN denotes clones
T5253 1756-1760 IN denotes from
T5254 1761-1765 DT denotes each
T5255 1766-1772 NN denotes sample
T5256 1773-1777 VB denotes were
T5257 1778-1787 VB denotes sequenced
T5258 1788-1792 IN denotes with
T5259 1793-1795 DT denotes an
T5260 1796-1805 VB denotes automated
T5261 1806-1815 NN denotes sequencer
T5262 1816-1817 -LRB- denotes (
T5263 1817-1820 NNP denotes ABI
T5264 1821-1826 NNP denotes Prism
T5265 1827-1830 CD denotes 377
T5266 1830-1831 -COMMA- denotes ,
T5267 1832-1839 NNP denotes Applied
T5268 1840-1850 NNP denotes Biosystems
T5269 1850-1851 -RRB- denotes )
R4058 T4944 T4943 arg1Of treatment,Bisulfite
R4059 T4945 T4946 arg1Of DNA,was
R4060 T4945 T4947 arg2Of DNA,extracted
R4061 T4947 T4946 arg2Of extracted,was
R4062 T4947 T4948 arg1Of extracted,as
R4063 T4949 T4948 arg2Of described,as
R4064 T4949 T4950 arg1Of described,above
R4065 T4952 T4951 arg1Of treatment,Bisulfite
R4066 T4952 T4953 arg1Of treatment,of
R4067 T4952 T4955 arg1Of treatment,","
R4068 T4952 T4956 arg1Of treatment,leading
R4069 T4952 T4962 modOf treatment,to
R4070 T4952 T4978 arg1Of treatment,follows
R4071 T4954 T4953 arg2Of DNA,of
R4072 T4956 T4957 arg1Of leading,to
R4073 T4958 T4957 arg2Of conversion,to
R4074 T4958 T4959 arg1Of conversion,of
R4075 T4961 T4959 arg2Of cytosine,of
R4076 T4961 T4960 arg1Of cytosine,unmethylated
R4077 T4963 T4972 arg1Of uracil,","
R4078 T4964 T4965 arg1Of residues,and
R4079 T4965 T4963 arg2Of and,uracil
R4080 T4967 T4965 arg2Of change,and
R4081 T4967 T4966 arg1Of change,no
R4082 T4967 T4968 arg1Of change,of
R4083 T4971 T4968 arg2Of residues,of
R4084 T4971 T4969 arg2Of residues,methylated
R4085 T4971 T4970 arg1Of residues,cytosine
R4086 T4972 T4962 arg1Of ",",to
R4087 T4974 T4972 arg2Of performed,","
R4088 T4974 T4973 arg2Of performed,was
R4089 T4974 T4975 arg1Of performed,as
R4090 T4976 T4975 arg2Of described,as
R4091 T4976 T4977 arg1Of described,as
R4092 T4982 T4981 arg1Of µg,1
R4093 T4982 T4983 arg1Of µg,of
R4094 T4982 T4988 arg1Of µg,of
R4095 T4982 T5004 arg1Of µg,were
R4096 T4982 T5005 arg2Of µg,denaturated
R4097 T4984 T4985 arg1Of DNA,and
R4098 T4985 T4983 arg2Of and,of
R4099 T4987 T4985 arg2Of µg,and
R4100 T4987 T4986 arg1Of µg,2
R4101 T4989 T4990 arg1Of poly,(
R4102 T4991 T4990 arg2Of dA–dT,(
R4103 T4992 T4990 arg3Of ),(
R4104 T4996 T4995 arg2Of dA–dT,(
R4105 T4997 T4995 arg3Of ),(
R4106 T4998 T4988 arg2Of copolymers,of
R4107 T4998 T4989 arg1Of copolymers,poly
R4108 T4998 T4993 arg1Of copolymers,(
R4109 T4998 T4994 arg1Of copolymers,poly
R4110 T4998 T4995 arg1Of copolymers,(
R4111 T4998 T4999 arg1Of copolymers,(
R4112 T5002 T4999 arg2Of Biotech,(
R4113 T5002 T5000 arg1Of Biotech,Amersham
R4114 T5002 T5001 arg1Of Biotech,Pharmacia
R4115 T5003 T4999 arg3Of ),(
R4116 T5005 T4979 arg1Of denaturated,Briefly
R4117 T5005 T4980 arg1Of denaturated,","
R4118 T5005 T5004 arg2Of denaturated,were
R4119 T5005 T5006 arg1Of denaturated,for
R4120 T5005 T5009 arg1Of denaturated,at
R4121 T5005 T5011 arg1Of denaturated,in
R4122 T5005 T5015 arg1Of denaturated,in
R4123 T5008 T5006 arg2Of min,for
R4124 T5008 T5007 arg1Of min,20
R4125 T5010 T5009 arg2Of 42°C,at
R4126 T5012 T5013 arg1Of 0.3,M
R4127 T5014 T5011 arg2Of NaOH,in
R4128 T5014 T5012 arg1Of NaOH,0.3
R4129 T5017 T5015 arg2Of volume,in
R4130 T5017 T5016 arg1Of volume,a
R4131 T5017 T5018 arg1Of volume,of
R4132 T5020 T5018 arg2Of µl,of
R4133 T5020 T5019 arg1Of µl,50
R4134 T5022 T5021 arg1Of solutions,Fresh
R4135 T5022 T5023 arg1Of solutions,of
R4136 T5022 T5047 arg1Of solutions,were
R4137 T5022 T5048 arg2Of solutions,added
R4138 T5025 T5024 arg1Of µl,30
R4139 T5025 T5026 arg1Of µl,of
R4140 T5025 T5033 arg1Of µl,and
R4141 T5027 T5028 arg1Of 10,mM
R4142 T5029 T5026 arg2Of hydrochinon,of
R4143 T5029 T5027 arg1Of hydrochinon,10
R4144 T5029 T5030 arg1Of hydrochinon,(
R4145 T5031 T5030 arg2Of Sigma,(
R4146 T5032 T5030 arg3Of ),(
R4147 T5033 T5023 arg2Of and,of
R4148 T5035 T5033 arg2Of µl,and
R4149 T5035 T5034 arg1Of µl,530
R4150 T5035 T5036 arg1Of µl,of
R4151 T5037 T5038 arg1Of 3,M
R4152 T5040 T5036 arg2Of bisulfite,of
R4153 T5040 T5037 arg1Of bisulfite,3
R4154 T5040 T5039 arg1Of bisulfite,sodium
R4155 T5040 T5041 arg1Of bisulfite,(
R4156 T5042 T5041 arg2Of pH,(
R4157 T5042 T5043 arg1Of pH,5.0
R4158 T5042 T5044 arg1Of pH,;
R4159 T5045 T5044 arg2Of Sigma,;
R4160 T5046 T5041 arg3Of ),(
R4161 T5048 T5047 arg2Of added,were
R4162 T5051 T5050 arg1Of solution,the
R4163 T5051 T5052 arg1Of solution,was
R4164 T5051 T5054 arg2Of solution,mixed
R4165 T5051 T5056 arg2Of solution,overlayed
R4166 T5051 T5061 arg2Of solution,incubated
R4167 T5054 T5055 arg1Of mixed,","
R4168 T5055 T5060 arg1Of ",",and
R4169 T5056 T5055 arg2Of overlayed,","
R4170 T5056 T5057 arg1Of overlayed,with
R4171 T5059 T5057 arg2Of oil,with
R4172 T5059 T5058 arg1Of oil,mineral
R4173 T5060 T5049 arg1Of and,","
R4174 T5060 T5052 arg2Of and,was
R4175 T5060 T5053 arg1Of and,gently
R4176 T5061 T5060 arg2Of incubated,and
R4177 T5061 T5062 arg1Of incubated,in
R4178 T5064 T5062 arg2Of dark,in
R4179 T5064 T5063 arg1Of dark,the
R4180 T5067 T5066 arg1Of h,12–13
R4181 T5067 T5068 arg1Of h,at
R4182 T5067 T5073 arg1Of h,was
R4183 T5067 T5074 arg2Of h,recovered
R4184 T5072 T5068 arg2Of phase,at
R4185 T5072 T5069 arg1Of phase,50°C.
R4186 T5072 T5070 arg1Of phase,The
R4187 T5072 T5071 arg1Of phase,aqueous
R4188 T5074 T5073 arg2Of recovered,was
R4189 T5074 T5075 modOf recovered,using
R4190 T5074 T5081 arg1Of recovered,(
R4191 T5080 T5075 arg2Of system’,using
R4192 T5080 T5076 arg1Of system’,the
R4193 T5080 T5077 arg1Of system’,‘Wizard
R4194 T5080 T5078 arg1Of system’,DNA
R4195 T5080 T5079 arg1Of system’,clean-up
R4196 T5082 T5083 arg1Of Promega,","
R4197 T5083 T5081 arg2Of ",",(
R4198 T5083 T5085 arg1Of ",",","
R4199 T5084 T5083 arg2Of Mannheim,","
R4200 T5086 T5085 arg2Of Germany,","
R4201 T5087 T5081 arg3Of ),(
R4202 T5090 T5088 arg1Of DNA,The
R4203 T5090 T5089 arg2Of DNA,purified
R4204 T5090 T5091 arg1Of DNA,was
R4205 T5090 T5093 arg2Of DNA,mixed
R4206 T5090 T5106 arg2Of DNA,incubated
R4207 T5093 T5094 arg1Of mixed,with
R4208 T5093 T5105 arg1Of mixed,and
R4209 T5095 T5096 arg1Of 1,M
R4210 T5097 T5094 arg2Of NaOH,with
R4211 T5097 T5095 arg1Of NaOH,1
R4212 T5097 T5098 arg1Of NaOH,to
R4213 T5101 T5098 arg2Of concentration,to
R4214 T5101 T5099 arg1Of concentration,a
R4215 T5101 T5100 arg1Of concentration,final
R4216 T5101 T5102 arg1Of concentration,of
R4217 T5104 T5102 arg2Of M,of
R4218 T5104 T5103 arg1Of M,0.3
R4219 T5105 T5091 arg2Of and,was
R4220 T5105 T5092 arg1Of and,subsequently
R4221 T5106 T5105 arg2Of incubated,and
R4222 T5106 T5107 arg1Of incubated,for
R4223 T5106 T5110 arg1Of incubated,at
R4224 T5106 T5112 modOf incubated,to
R4225 T5109 T5107 arg2Of min,for
R4226 T5109 T5108 arg1Of min,20
R4227 T5111 T5110 arg2Of 37°C,at
R4228 T5113 T5112 arg1Of ensure,to
R4229 T5115 T5113 arg2Of desulfonisation,ensure
R4230 T5115 T5114 arg1Of desulfonisation,complete
R4231 T5116 T5117 arg1Of DNA,was
R4232 T5116 T5138 arg1Of DNA,resuspended
R4233 T5117 T5137 arg1Of was,and
R4234 T5118 T5117 arg2Of ethanol,was
R4235 T5118 T5119 arg2Of ethanol,precipitated
R4236 T5119 T5120 arg1Of precipitated,in
R4237 T5122 T5120 arg2Of presence,in
R4238 T5122 T5121 arg1Of presence,the
R4239 T5122 T5123 arg1Of presence,of
R4240 T5122 T5131 arg1Of presence,","
R4241 T5122 T5132 arg2Of presence,washed
R4242 T5125 T5123 arg2Of vol,of
R4243 T5125 T5124 arg1Of vol,1/10
R4244 T5125 T5126 arg1Of vol,of
R4245 T5127 T5128 arg1Of 3,M
R4246 T5130 T5126 arg2Of acetate,of
R4247 T5130 T5127 arg1Of acetate,3
R4248 T5130 T5129 arg1Of acetate,sodium
R4249 T5132 T5133 arg1Of washed,with
R4250 T5134 T5135 arg1Of 70,%
R4251 T5136 T5133 arg2Of ethanol,with
R4252 T5136 T5134 arg1Of ethanol,70
R4253 T5138 T5137 arg2Of resuspended,and
R4254 T5138 T5139 arg1Of resuspended,in
R4255 T5142 T5139 arg2Of H2O,in
R4256 T5142 T5140 arg1Of H2O,50
R4257 T5142 T5141 arg1Of H2O,µl
R4258 T5145 T5143 arg1Of amplification,Subsequent
R4259 T5145 T5144 arg1Of amplification,PCR
R4260 T5145 T5146 arg1Of amplification,of
R4261 T5145 T5151 arg1Of amplification,was
R4262 T5145 T5152 arg2Of amplification,used
R4263 T5150 T5146 arg2Of DNA,of
R4264 T5150 T5147 arg1Of DNA,4
R4265 T5150 T5148 arg1Of DNA,µl
R4266 T5150 T5149 arg1Of DNA,bisulfite-treated
R4267 T5152 T5151 arg2Of used,was
R4268 T5152 T5153 arg1Of used,for
R4269 T5152 T5167 arg1Of used,into
R4270 T5152 T5179 arg1Of used,(
R4271 T5154 T5153 arg2Of cloning,for
R4272 T5154 T5155 arg1Of cloning,of
R4273 T5157 T5155 arg2Of fragments,of
R4274 T5157 T5156 arg1Of fragments,two
R4275 T5157 T5158 arg1Of fragments,of
R4276 T5157 T5162 arg1Of fragments,(
R4277 T5161 T5158 arg2Of promoter,of
R4278 T5161 T5159 arg1Of promoter,the
R4279 T5161 T5160 arg1Of promoter,IRF-4
R4280 T5163 T5164 arg1Of BS-I,and
R4281 T5164 T5162 arg2Of and,(
R4282 T5165 T5164 arg2Of BS-II,and
R4283 T5166 T5162 arg3Of ),(
R4284 T5169 T5167 arg2Of vector,into
R4285 T5169 T5168 arg1Of vector,pCR2.1
R4286 T5169 T5170 arg1Of vector,with
R4287 T5175 T5170 arg2Of kit’,with
R4288 T5175 T5171 arg1Of kit’,the
R4289 T5175 T5172 arg1Of kit’,‘TOPO
R4290 T5175 T5173 arg1Of kit’,TA
R4291 T5175 T5174 arg1Of kit’,cloning
R4292 T5175 T5176 arg1Of kit’,(
R4293 T5177 T5176 arg2Of Invitrogen,(
R4294 T5178 T5176 arg3Of ),(
R4295 T5180 T5179 arg2Of see,(
R4296 T5182 T5180 arg2Of 3A,see
R4297 T5182 T5181 arg1Of 3A,Figure
R4298 T5183 T5179 arg3Of ),(
R4299 T5185 T5184 arg1Of primers,The
R4300 T5185 T5186 arg2Of primers,used
R4301 T5185 T5196 arg1Of primers,contain
R4302 T5186 T5187 arg1Of used,for
R4303 T5189 T5187 arg2Of amplification,for
R4304 T5189 T5188 arg1Of amplification,PCR
R4305 T5189 T5190 arg1Of amplification,of
R4306 T5192 T5193 arg1Of BS-I,and
R4307 T5194 T5193 arg2Of BS-II,and
R4308 T5195 T5190 arg2Of fragments,of
R4309 T5195 T5191 arg1Of fragments,the
R4310 T5195 T5192 arg1Of fragments,BS-I
R4311 T5195 T5194 arg1Of fragments,BS-II
R4312 T5196 T5219 arg1Of contain,:
R4313 T5196 T5221 arg1Of contain,5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′
R4314 T5196 T5229 arg1Of contain,;
R4315 T5196 T5231 arg1Of contain,5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′
R4316 T5200 T5196 arg2Of sequence,contain
R4317 T5200 T5197 arg1Of sequence,the
R4318 T5200 T5198 arg1Of sequence,putative
R4319 T5200 T5199 arg1Of sequence,altered
R4320 T5200 T5201 arg1Of sequence,of
R4321 T5204 T5201 arg2Of strand,of
R4322 T5204 T5202 arg1Of strand,the
R4323 T5204 T5203 arg1Of strand,sense
R4324 T5204 T5205 arg1Of strand,due
R4325 T5207 T5205 arg2Of bisulfite,due
R4326 T5207 T5206 arg1Of bisulfite,to
R4327 T5208 T5207 arg2Of treatment,bisulfite
R4328 T5208 T5209 arg1Of treatment,(
R4329 T5212 T5210 arg2Of residues,converted
R4330 T5212 T5211 arg1Of residues,cytosine
R4331 T5212 T5213 arg1Of residues,are
R4332 T5212 T5214 arg2Of residues,written
R4333 T5214 T5209 arg2Of written,(
R4334 T5214 T5213 arg2Of written,are
R4335 T5214 T5215 arg1Of written,in
R4336 T5217 T5215 arg2Of letters,in
R4337 T5217 T5216 arg1Of letters,bold
R4338 T5218 T5209 arg3Of ),(
R4339 T5221 T5220 arg1Of 5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′,BS-I-forward
R4340 T5221 T5222 arg1Of 5′-TATTTGGATTTTTAGGGAGTTTTTTTT-3′,","
R4341 T5224 T5222 arg2Of 5′-ACCCAACTCCCTTAAACTATTAAACT-3′,","
R4342 T5224 T5223 arg1Of 5′-ACCCAACTCCCTTAAACTATTAAACT-3′,BS-I-reverse
R4343 T5224 T5225 arg1Of 5′-ACCCAACTCCCTTAAACTATTAAACT-3′,(
R4344 T5227 T5225 arg2Of bp,(
R4345 T5227 T5226 arg1Of bp,187
R4346 T5228 T5225 arg3Of ),(
R4347 T5231 T5230 arg1Of 5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′,BS-II-forward
R4348 T5231 T5232 arg1Of 5′-AGTTTAATAGTTTAAGGGAGTTGGGT-3′,","
R4349 T5234 T5232 arg2Of 5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,","
R4350 T5234 T5233 arg1Of 5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,BS-II-reverse
R4351 T5234 T5235 arg1Of 5′-CTCACCCTAAACTCAAAACTAAAAAC-3′,(
R4352 T5237 T5235 arg2Of bp,(
R4353 T5237 T5236 arg1Of bp,674
R4354 T5238 T5235 arg3Of ),(
R4355 T5241 T5239 arg2Of amplification,After
R4356 T5241 T5240 arg1Of amplification,bacterial
R4357 T5241 T5242 arg1Of amplification,of
R4358 T5241 T5247 arg1Of amplification,by
R4359 T5246 T5242 arg2Of fragments,of
R4360 T5246 T5243 arg1Of fragments,the
R4361 T5246 T5244 arg2Of fragments,cloned
R4362 T5246 T5245 arg1Of fragments,PCR
R4363 T5249 T5247 arg2Of procedures,by
R4364 T5249 T5248 arg1Of procedures,standard
R4365 T5252 T5251 arg1Of clones,eight
R4366 T5252 T5253 arg1Of clones,from
R4367 T5252 T5256 arg1Of clones,were
R4368 T5252 T5257 arg2Of clones,sequenced
R4369 T5255 T5253 arg2Of sample,from
R4370 T5255 T5254 arg1Of sample,each
R4371 T5257 T5239 arg1Of sequenced,After
R4372 T5257 T5250 arg1Of sequenced,","
R4373 T5257 T5256 arg2Of sequenced,were
R4374 T5257 T5258 arg1Of sequenced,with
R4375 T5257 T5262 arg1Of sequenced,(
R4376 T5261 T5258 arg2Of sequencer,with
R4377 T5261 T5259 arg1Of sequencer,an
R4378 T5261 T5260 arg2Of sequencer,automated
R4379 T5264 T5262 arg2Of Prism,(
R4380 T5264 T5263 arg1Of Prism,ABI
R4381 T5264 T5265 arg1Of Prism,377
R4382 T5264 T5266 arg1Of Prism,","
R4383 T5268 T5266 arg2Of Biosystems,","
R4384 T5268 T5267 arg1Of Biosystems,Applied
R4385 T5269 T5262 arg3Of ),(