PMC:1134658 / 22781-29525 JSONTXT 17 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T13708 9-13 NN denotes Cell
T13709 14-21 NN denotes culture
T13710 22-24 IN denotes If
T13711 25-28 RB denotes not
T13712 29-38 VB denotes specified
T13713 38-39 -COMMA- denotes ,
T13714 40-43 DT denotes all
T13715 44-49 NN denotes cells
T13716 50-54 VB denotes were
T13717 55-63 VB denotes cultured
T13718 64-66 IN denotes in
T13719 67-73 NN denotes X-VIVO
T13720 74-77 CD denotes 15™
T13721 78-79 -LRB- denotes (
T13722 79-91 NNP denotes BioWhittaker
T13723 91-92 -COMMA- denotes ,
T13724 93-101 NNP denotes Verviers
T13725 101-102 -COMMA- denotes ,
T13726 103-110 NNP denotes Belgium
T13727 110-111 -RRB- denotes )
T13728 112-122 JJ denotes serum-free
T13729 123-129 NN denotes medium
T13730 130-132 IN denotes at
T13731 133-137 NN denotes 37°C
T13732 138-141 CC denotes and
T13733 142-143 CD denotes 5
T13734 143-144 NN denotes %
T13735 145-148 NN denotes CO2
T13736 149-151 IN denotes in
T13737 152-155 NN denotes air
T13738 157-167 JJ denotes Peripheral
T13739 168-173 NN denotes blood
T13740 174-177 VB denotes was
T13741 178-186 VB denotes provided
T13742 187-189 IN denotes by
T13743 190-193 DT denotes the
T13744 194-199 NNP denotes Blood
T13745 200-204 NNP denotes Bank
T13746 205-207 IN denotes at
T13747 208-216 NNP denotes Buskerud
T13748 217-225 NNP denotes Regional
T13749 226-234 NNP denotes Hospital
T13750 235-239 IN denotes with
T13751 240-246 JJ denotes formal
T13752 247-256 NN denotes agreement
T13753 257-259 IN denotes by
T13754 260-263 DT denotes the
T13755 264-272 NN denotes patients
T13756 272-273 -COMMA- denotes ,
T13757 274-277 CC denotes and
T13758 278-286 NN denotes approval
T13759 287-289 IN denotes by
T13760 290-293 DT denotes the
T13761 294-302 JJ denotes regional
T13762 303-309 NN denotes ethics
T13763 310-319 NN denotes committee
T13764 321-327 RB denotes Highly
T13765 328-336 VB denotes purified
T13766 337-344 VB denotes resting
T13767 345-350 JJ denotes human
T13768 351-364 NN denotes B-lymphocytes
T13769 365-366 -LRB- denotes (
T13770 366-371 JJ denotes CD19+
T13771 372-377 NN denotes cells
T13772 377-378 -RRB- denotes )
T13773 379-383 VB denotes were
T13774 384-392 VB denotes isolated
T13775 393-397 IN denotes from
T13776 398-401 DT denotes the
T13777 402-412 JJ denotes peripheral
T13778 413-418 NN denotes blood
T13779 419-421 IN denotes by
T13780 422-431 VB denotes rosetting
T13781 432-436 IN denotes with
T13782 437-451 JJ denotes immunomagnetic
T13783 452-457 NN denotes beads
T13784 458-459 -LRB- denotes (
T13785 459-468 NN denotes Dynabeads
T13786 469-473 NN denotes M450
T13787 473-474 -COLON- denotes ;
T13788 475-480 NNP denotes Dynal
T13789 480-481 -COMMA- denotes ,
T13790 482-486 NNP denotes Oslo
T13791 486-487 -COMMA- denotes ,
T13792 488-494 NNP denotes Norway
T13793 494-495 -RRB- denotes )
T13794 496-498 IN denotes as
T13795 499-508 VB denotes described
T13796 509-510 -LRB- denotes [
T13797 510-512 CD denotes 55
T13798 512-513 -RRB- denotes ]
T13799 515-519 DT denotes This
T13800 520-529 NN denotes procedure
T13801 530-536 VB denotes yields
T13802 537-541 JJ denotes less
T13803 542-546 IN denotes than
T13804 547-550 CD denotes 0.5
T13805 550-551 NN denotes %
T13806 552-553 NN denotes T
T13807 554-559 NN denotes cells
T13808 559-560 -COMMA- denotes ,
T13809 561-564 CD denotes 0.1
T13810 564-565 NN denotes %
T13811 566-568 NN denotes NK
T13812 569-574 NN denotes cells
T13813 574-575 -COMMA- denotes ,
T13814 576-579 CC denotes and
T13815 580-583 CD denotes 0.5
T13816 583-584 NN denotes %
T13817 585-594 NN denotes monocytes
T13818 595-597 IN denotes as
T13819 598-604 VB denotes judged
T13820 605-607 IN denotes by
T13821 608-616 JJ denotes indirect
T13822 617-635 NN denotes immunofluorescence
T13823 636-644 NN denotes staining
T13824 646-649 DT denotes The
T13825 650-659 VB denotes following
T13826 660-664 NN denotes cell
T13827 665-670 NN denotes lines
T13828 671-675 IN denotes from
T13829 676-681 JJ denotes human
T13830 682-690 JJ denotes lymphoid
T13831 691-703 NN denotes malignancies
T13832 704-708 VB denotes were
T13833 709-719 VB denotes maintained
T13834 720-722 IN denotes in
T13835 723-727 NN denotes RPMI
T13836 728-732 CD denotes 1640
T13837 733-734 -LRB- denotes (
T13838 734-737 NN denotes PAA
T13839 738-750 NNP denotes Laboratories
T13840 751-755 NNP denotes GmbH
T13841 755-756 -COMMA- denotes ,
T13842 757-765 NNP denotes Pasching
T13843 765-766 -COMMA- denotes ,
T13844 767-774 NNP denotes Austria
T13845 774-775 -RRB- denotes )
T13846 776-788 VB denotes supplemented
T13847 789-793 IN denotes with
T13848 794-796 CD denotes 10
T13849 796-797 NN denotes %
T13850 798-804 JJ denotes foetal
T13851 805-811 JJ denotes bovine
T13852 812-817 NN denotes serum
T13853 818-819 -LRB- denotes (
T13854 819-822 NN denotes FCS
T13855 822-823 -RRB- denotes )
T13856 823-824 -COMMA- denotes ,
T13857 825-828 CD denotes 100
T13858 829-837 NN denotes units/ml
T13859 838-848 NN denotes penicillin
T13860 849-850 NN denotes G
T13861 850-851 -COMMA- denotes ,
T13862 852-855 CC denotes and
T13863 856-859 CD denotes 100
T13864 860-868 NN denotes units/ml
T13865 869-871 IN denotes of
T13866 872-884 NN denotes streptomycin
T13867 885-893 NN denotes sulphate
T13868 893-894 -COMMA- denotes ,
T13869 895-898 CC denotes but
T13870 899-912 JJ denotes serum-starved
T13871 913-916 IN denotes for
T13872 917-919 IN denotes at
T13873 920-925 JJ denotes least
T13874 926-930 CD denotes four
T13875 931-936 NN denotes hours
T13876 937-940 CC denotes and
T13877 941-949 VB denotes cultured
T13878 950-952 IN denotes in
T13879 953-959 NN denotes X-VIVO
T13880 960-963 CD denotes 15™
T13881 964-968 WRB denotes when
T13882 969-977 VB denotes included
T13883 978-980 IN denotes in
T13884 981-992 NN denotes experiments
T13885 992-993 -COLON- denotes :
T13886 994-1006 JJ denotes EBV-negative
T13887 1007-1009 NN denotes BL
T13888 1010-1014 NN denotes cell
T13889 1015-1020 NN denotes lines
T13890 1021-1026 NN denotes Ramos
T13891 1027-1028 -LRB- denotes (
T13892 1028-1033 NN denotes ECACC
T13893 1034-1042 CD denotes 85030802
T13894 1042-1043 -RRB- denotes )
T13895 1043-1044 -COMMA- denotes ,
T13896 1045-1049 NN denotes HL60
T13897 1050-1051 -LRB- denotes (
T13898 1051-1059 NN denotes JCRB0085
T13899 1059-1060 -RRB- denotes )
T14428 1606-1616 NN denotes Antibodies
T14429 1617-1621 VB denotes used
T14430 1622-1625 IN denotes for
T14431 1626-1630 NN denotes flow
T14432 1631-1641 JJ denotes cytometric
T14433 1642-1650 NN denotes analysis
T14434 1651-1654 CC denotes and
T14435 1655-1665 NN denotes immunoblot
T14436 1666-1674 NN denotes analysis
T14437 1675-1685 NN denotes Antibodies
T14438 1686-1693 IN denotes against
T14439 1694-1697 DT denotes the
T14440 1698-1703 JJ denotes human
T14441 1704-1717 NN denotes BMP-receptors
T14442 1718-1725 NN denotes Act-RIA
T14443 1725-1726 -COMMA- denotes ,
T14444 1727-1734 NN denotes BMP-RIB
T14445 1734-1735 -COMMA- denotes ,
T14446 1736-1743 NN denotes BMPR-II
T14447 1743-1744 -COMMA- denotes ,
T14448 1745-1753 NN denotes Act-RIIA
T14449 1754-1757 CC denotes and
T14450 1758-1766 NN denotes Act-RIIb
T14451 1767-1771 VB denotes were
T14452 1772-1781 VB denotes purchased
T14453 1782-1786 IN denotes from
T14454 1787-1788 NNP denotes R
T14455 1789-1790 CC denotes &
T14456 1791-1792 NNP denotes D
T14457 1793-1800 NNP denotes Systems
T14458 1801-1802 -LRB- denotes (
T14459 1802-1810 NNP denotes Abingdon
T14460 1810-1811 -COMMA- denotes ,
T14461 1812-1814 NNP denotes UK
T14462 1814-1815 -RRB- denotes )
T14463 1817-1826 NN denotes Detection
T14464 1827-1829 IN denotes of
T14465 1830-1833 DT denotes the
T14466 1834-1847 NN denotes BMP-6-protein
T14467 1848-1851 VB denotes has
T14468 1852-1856 VB denotes been
T14469 1857-1862 VB denotes tried
T14470 1863-1867 IN denotes with
T14471 1868-1871 DT denotes the
T14472 1872-1881 VB denotes following
T14473 1882-1892 NN denotes antibodies
T14474 1892-1893 -COLON- denotes :
T14475 1894-1898 NN denotes goat
T14476 1899-1909 JJ denotes polyclonal
T14477 1910-1920 NN denotes anti-BMP-6
T14478 1921-1922 -LRB- denotes (
T14479 1922-1927 NNP denotes Santa
T14480 1928-1932 NNP denotes Cruz
T14481 1932-1933 -COMMA- denotes ,
T14482 1934-1937 NNP denotes San
T14483 1938-1943 NNP denotes Diego
T14484 1943-1944 -COMMA- denotes ,
T14485 1945-1947 NNP denotes CA
T14486 1947-1948 -COMMA- denotes ,
T14487 1949-1952 NNP denotes USA
T14488 1952-1953 -RRB- denotes )
T14489 1953-1954 -COMMA- denotes ,
T14490 1955-1965 JJ denotes monoclonal
T14491 1966-1971 NN denotes mouse
T14492 1972-1982 NN denotes anti-BMP-6
T14493 1983-1986 CC denotes and
T14494 1987-1997 JJ denotes polyclonal
T14495 1998-2002 NN denotes goat
T14496 2003-2013 NN denotes anti-BMP-6
T14497 2014-2018 IN denotes from
T14498 2019-2020 NNP denotes R
T14499 2021-2022 CC denotes &
T14500 2023-2024 NNP denotes D
T14501 2025-2032 NNP denotes Systems
T14502 2033-2034 -LRB- denotes (
T14503 2034-2042 NNP denotes Abingdon
T14504 2042-2043 -COMMA- denotes ,
T14505 2044-2046 NNP denotes UK
T14506 2046-2047 -RRB- denotes )
T14507 2047-2048 -COMMA- denotes ,
T14508 2049-2052 CC denotes and
T14509 2053-2058 NN denotes mouse
T14510 2059-2069 JJ denotes monoclonal
T14511 2070-2080 JJ denotes anti-BMP-6
T14512 2081-2082 -LRB- denotes (
T14513 2082-2090 NNP denotes Chemicon
T14514 2091-2104 NNP denotes International
T14515 2105-2108 NNP denotes Inc
T14516 2108-2109 -COMMA- denotes ,
T14517 2110-2118 NNP denotes Temecula
T14518 2118-2119 -COMMA- denotes ,
T14519 2120-2122 NNP denotes CA
T14520 2122-2123 -COMMA- denotes ,
T14521 2124-2127 NNP denotes USA
T14522 2127-2128 -RRB- denotes )
T14523 2130-2146 NN denotes Characterisation
T14524 2147-2149 IN denotes of
T14525 2150-2164 JJ denotes BMP-signalling
T14526 2165-2173 NN denotes pathways
T14527 2174-2177 VB denotes was
T14528 2178-2182 VB denotes done
T14529 2183-2185 IN denotes by
T14530 2186-2189 NN denotes use
T14531 2190-2192 IN denotes of
T14532 2193-2211 NN denotes anti-phospho-Smad1
T14533 2211-2212 -COMMA- denotes ,
T14534 2213-2215 CD denotes -5
T14535 2215-2216 -COMMA- denotes ,
T14536 2217-2221 JJ denotes -and
T14537 2222-2223 CD denotes 8
T14538 2224-2234 JJ denotes polyclonal
T14539 2235-2243 NN denotes antibody
T14540 2244-2245 -LRB- denotes (
T14541 2245-2253 NNP denotes Chemicon
T14542 2253-2254 -COMMA- denotes ,
T14543 2255-2263 NNP denotes Temecula
T14544 2263-2264 -COMMA- denotes ,
T14545 2265-2267 NNP denotes CA
T14546 2267-2268 -COMMA- denotes ,
T14547 2269-2272 NNP denotes USA
T14548 2272-2273 -RRB- denotes )
T14549 2275-2285 NN denotes Expression
T14550 2286-2292 NN denotes levels
T14551 2293-2295 IN denotes of
T14552 2296-2301 NN denotes Id1–3
T14553 2302-2310 NN denotes proteins
T14554 2311-2315 VB denotes were
T14555 2316-2324 VB denotes detected
T14556 2325-2329 IN denotes with
T14557 2330-2340 JJ denotes polyclonal
T14558 2341-2347 NN denotes rabbit
T14559 2348-2356 NN denotes antibody
T14560 2357-2360 CC denotes and
T14561 2361-2370 NN denotes detection
T14562 2371-2374 VB denotes was
T14563 2375-2382 VB denotes blocked
T14564 2383-2387 IN denotes with
T14565 2388-2396 VB denotes blocking
T14566 2397-2404 NN denotes peptide
T14567 2405-2409 IN denotes from
T14568 2410-2415 NNP denotes Santa
T14569 2416-2420 NNP denotes Cruz
T14570 2421-2434 NNP denotes Biotechnology
T14571 2435-2436 -LRB- denotes (
T14572 2436-2441 NNP denotes Santa
T14573 2442-2446 NNP denotes Cruz
T14574 2446-2447 -COMMA- denotes ,
T14575 2448-2451 NNP denotes San
T14576 2452-2457 NNP denotes Diego
T14577 2457-2458 -COMMA- denotes ,
T14578 2459-2461 NNP denotes CA
T14579 2461-2462 -COMMA- denotes ,
T14580 2463-2466 NNP denotes USA
T14581 2466-2467 -RRB- denotes )
T14582 2469-2471 IN denotes As
T14583 2472-2481 JJ denotes secondary
T14584 2482-2492 NN denotes antibodies
T14585 2493-2499 VB denotes served
T14586 2500-2510 JJ denotes anti-mouse
T14587 2510-2511 -COMMA- denotes ,
T14588 2512-2521 JJ denotes anti-goat
T14589 2522-2524 CC denotes or
T14590 2525-2536 JJ denotes anti-rabbit
T14591 2537-2541 NN denotes IgG-
T14592 2542-2553 NN denotes horseradish
T14593 2554-2564 NN denotes peroxidase
T14594 2565-2566 -LRB- denotes (
T14595 2566-2569 NN denotes HRP
T14596 2569-2570 -RRB- denotes )
T14597 2571-2575 IN denotes from
T14598 2576-2590 NN denotes Dakocytomation
T14599 2591-2593 NN denotes AS
T14600 2594-2595 -LRB- denotes (
T14601 2595-2605 NN denotes Copenhagen
T14602 2605-2606 -COMMA- denotes ,
T14603 2607-2614 NNP denotes Denmark
T14604 2614-2615 -RRB- denotes )
T14605 2616-2619 IN denotes for
T14606 2620-2630 NN denotes immunoblot
T14607 2631-2639 NN denotes analysis
T14608 2641-2653 NN denotes Anti-β-actin
T14609 2654-2657 VB denotes was
T14610 2658-2662 IN denotes from
T14611 2663-2674 NNP denotes Santa-Cruz.
T14612 2675-2679 NNP denotes From
T14613 2680-2686 NNP denotes Becton
T14614 2687-2696 NNP denotes Dickinson
T14615 2697-2698 -LRB- denotes (
T14616 2698-2701 NNP denotes San
T14617 2702-2706 NNP denotes Jose
T14618 2706-2707 -COMMA- denotes ,
T14619 2708-2710 NNP denotes CA
T14620 2710-2711 -RRB- denotes )
T14621 2711-2712 -COMMA- denotes ,
T14622 2713-2715 PRP denotes we
T14623 2716-2725 VB denotes purchased
T14624 2726-2738 JJ denotes anti-CD19-PE
T14625 2738-2739 -COMMA- denotes ,
T14626 2740-2754 NN denotes anti-CD19-FITC
T14627 2756-2759 DT denotes The
T14628 2760-2770 NN denotes antibodies
T14629 2771-2775 VB denotes used
T14630 2776-2779 IN denotes for
T14631 2780-2784 NN denotes cell
T14632 2785-2792 NN denotes sorting
T14633 2793-2797 VB denotes were
T14634 2798-2807 JJ denotes anti-CD19
T14635 2808-2811 NN denotes PC5
T14636 2812-2816 IN denotes from
T14637 2817-2827 NNP denotes Immunotech
T14638 2828-2830 NNP denotes SA
T14639 2831-2832 -LRB- denotes (
T14640 2832-2841 NNP denotes Marseille
T14641 2841-2842 -COMMA- denotes ,
T14642 2843-2849 NNP denotes France
T14643 2849-2850 -RRB- denotes )
T14644 2851-2854 CC denotes and
T14645 2855-2864 JJ denotes anti-CD27
T14646 2865-2867 NN denotes PE
T14647 2868-2872 IN denotes from
T14648 2873-2879 NNP denotes Becton
T14649 2880-2889 NNP denotes Dickinson
T14650 2889-2890 -COMMA- denotes ,
T14651 2891-2902 NNP denotes Biosciences
T14652 2903-2913 NNP denotes Pharmingen
T14653 2914-2915 -LRB- denotes (
T14654 2915-2918 NNP denotes San
T14655 2919-2924 NNP denotes Diego
T14656 2924-2925 -COMMA- denotes ,
T14657 2926-2928 NNP denotes CA
T14658 2928-2929 -COMMA- denotes ,
T14659 2930-2933 NNP denotes USA
T14660 2933-2934 -RRB- denotes )
T15102 2937-2941 NN denotes Cell
T15103 2942-2949 NN denotes sorting
T15104 2950-2956 RB denotes Highly
T15105 2957-2965 VB denotes purified
T15106 2966-2976 JJ denotes CD19+CD27-
T15107 2977-2979 CC denotes or
T15108 2980-2990 JJ denotes CD19+CD27+
T15109 2991-2996 NN denotes cells
T15110 2997-3001 VB denotes were
T15111 3002-3010 VB denotes obtained
T15112 3011-3013 IN denotes by
T15113 3014-3022 VB denotes staining
T15114 3023-3028 JJ denotes CD19+
T15115 3029-3034 NN denotes cells
T15116 3035-3039 IN denotes with
T15117 3040-3049 JJ denotes anti-CD27
T15118 3050-3052 NN denotes PE
T15119 3053-3056 CC denotes and
T15120 3057-3061 NN denotes CD19
T15121 3062-3065 NN denotes PC5
T15122 3066-3070 NN denotes mAbs
T15123 3071-3074 IN denotes for
T15124 3075-3077 CD denotes 30
T15125 3078-3085 NN denotes minutes
T15126 3086-3088 IN denotes at
T15127 3089-3092 NN denotes 4°C
T15128 3092-3093 -COMMA- denotes ,
T15129 3094-3102 VB denotes followed
T15130 3103-3105 IN denotes by
T15131 3106-3113 VB denotes washing
T15132 3114-3118 IN denotes with
T15133 3119-3122 NNP denotes PBS
T15134 3123-3126 CC denotes and
T15135 3127-3134 VB denotes sorting
T15136 3135-3137 IN denotes on
T15137 3138-3142 NNP denotes FACS
T15138 3143-3147 NNP denotes DiVa
T15139 3148-3152 IN denotes from
T15140 3153-3159 NNP denotes Becton
T15141 3160-3169 NNP denotes Dickinson
T15269 3172-3184 JJ denotes Western-blot
T15270 3185-3193 NN denotes analysis
T15271 3194-3195 NN denotes B
T15272 3196-3201 NN denotes cells
T15273 3202-3206 IN denotes from
T15274 3207-3217 JJ denotes peripheral
T15275 3218-3223 NN denotes blood
T15276 3224-3226 CC denotes or
T15277 3227-3235 VB denotes cultured
T15278 3236-3246 NN denotes cell-lines
T15279 3247-3251 VB denotes were
T15280 3252-3257 VB denotes lysed
T15281 3258-3260 IN denotes in
T15282 3261-3266 NN denotes lysis
T15283 3267-3273 NN denotes buffer
T15284 3274-3275 -LRB- denotes (
T15285 3275-3283 NN denotes glycerol
T15286 3284-3286 CD denotes 10
T15287 3286-3287 NN denotes %
T15288 3287-3288 -COMMA- denotes ,
T15289 3289-3306 NN denotes β-mercaptoethanol
T15290 3307-3308 CD denotes 5
T15291 3308-3309 NN denotes %
T15292 3309-3310 -COMMA- denotes ,
T15293 3311-3317 CD denotes 0.0625
T15294 3318-3319 NN denotes M
T15295 3320-3328 NN denotes Tris-HCL
T15296 3329-3330 -LRB- denotes [
T15297 3330-3332 NN denotes pH
T15298 3333-3336 CD denotes 6.8
T15299 3336-3337 -RRB- denotes ]
T15300 3337-3338 -COMMA- denotes ,
T15301 3339-3345 NN denotes sodium
T15302 3346-3353 NN denotes dodecyl
T15303 3354-3362 NN denotes sulphate
T15304 3363-3364 -LRB- denotes [
T15305 3364-3367 NN denotes SDS
T15306 3367-3368 -RRB- denotes ]
T15307 3369-3372 CD denotes 2.5
T15308 3372-3373 NN denotes %
T15309 3373-3378 NN denotes w/vol
T15310 3378-3379 -RRB- denotes )
T15311 3381-3386 JJ denotes Total
T15312 3387-3394 NN denotes protein
T15313 3395-3396 -LRB- denotes (
T15314 3396-3402 CD denotes 30–100
T15315 3403-3405 NN denotes μg
T15316 3405-3406 -RRB- denotes )
T15317 3407-3411 IN denotes from
T15318 3412-3416 DT denotes each
T15319 3417-3423 NN denotes sample
T15320 3424-3427 VB denotes was
T15321 3428-3431 VB denotes run
T15322 3432-3434 IN denotes on
T15323 3435-3437 CD denotes 10
T15324 3437-3438 NN denotes %
T15325 3439-3441 CC denotes or
T15326 3442-3444 CD denotes 12
T15327 3444-3445 NN denotes %
T15328 3446-3464 NN denotes SDS/polyacrylamide
T15329 3465-3466 -LRB- denotes (
T15330 3466-3474 NN denotes SDS/PAGE
T15331 3474-3475 -RRB- denotes )
T15332 3476-3480 NN denotes gels
T15333 3481-3484 CC denotes and
T15334 3485-3492 VB denotes blotted
T15335 3493-3497 IN denotes onto
T15336 3498-3512 NN denotes nitrocellulose
T15337 3513-3520 NN denotes filters
T15338 3521-3522 -LRB- denotes (
T15339 3522-3529 NNP denotes Protran
T15340 3529-3530 -COLON- denotes ;
T15341 3531-3541 NNP denotes Schleicher
T15342 3542-3543 CC denotes &
T15343 3543-3550 NNP denotes Schuell
T15344 3551-3555 NNP denotes GmbH
T15345 3555-3556 -COMMA- denotes ,
T15346 3557-3563 NNP denotes Dassel
T15347 3563-3564 -COMMA- denotes ,
T15348 3565-3572 NNP denotes Germany
T15349 3572-3573 -RRB- denotes )
T15350 3575-3583 NN denotes Blocking
T15351 3583-3584 -COMMA- denotes ,
T15352 3585-3592 NN denotes washing
T15353 3593-3596 CC denotes and
T15354 3597-3607 NN denotes incubation
T15355 3608-3610 IN denotes of
T15356 3611-3614 DT denotes the
T15357 3615-3622 NN denotes filters
T15358 3623-3627 IN denotes with
T15359 3628-3635 JJ denotes primary
T15360 3636-3646 NN denotes antibodies
T15361 3647-3651 VB denotes were
T15362 3652-3656 VB denotes done
T15363 3657-3666 VB denotes according
T15364 3667-3669 TO denotes to
T15365 3670-3673 DT denotes the
T15366 3674-3686 NN denotes manufacturer
T15367 3686-3688 POS denotes 's
T15368 3689-3698 NN denotes protocols
T15369 3699-3701 IN denotes at
T15370 3702-3706 NN denotes room
T15371 3707-3718 NN denotes temperature
T15372 3719-3720 -LRB- denotes (
T15373 3720-3722 NN denotes RT
T15374 3722-3723 -RRB- denotes )
T15375 3725-3730 IN denotes After
T15376 3731-3738 VB denotes washing
T15377 3739-3743 IN denotes with
T15378 3744-3751 NN denotes TBS/0.1
T15379 3751-3752 NN denotes %
T15380 3753-3761 CD denotes Tween-20
T15381 3762-3763 -LRB- denotes (
T15382 3763-3768 NN denotes TBS-T
T15383 3768-3769 -RRB- denotes )
T15384 3769-3770 -COMMA- denotes ,
T15385 3771-3774 DT denotes the
T15386 3775-3782 NN denotes filters
T15387 3783-3787 VB denotes were
T15388 3788-3797 VB denotes incubated
T15389 3798-3802 IN denotes with
T15390 3803-3814 NN denotes horseradish
T15391 3815-3825 NN denotes peroxidase
T15392 3826-3827 -LRB- denotes (
T15393 3827-3830 NN denotes HRP
T15394 3830-3831 -RRB- denotes )
T15395 3832-3839 VB denotes coupled
T15396 3840-3842 TO denotes to
T15397 3843-3851 JJ denotes relevant
T15398 3852-3861 JJ denotes secondary
T15399 3862-3872 NN denotes antibodies
T15400 3873-3874 -LRB- denotes (
T15401 3874-3877 VB denotes see
T15402 3878-3883 IN denotes above
T15403 3883-3884 -RRB- denotes )
T15404 3885-3888 IN denotes for
T15405 3889-3891 CD denotes 60
T15406 3892-3899 NN denotes minutes
T15407 3900-3902 IN denotes at
T15408 3903-3905 NN denotes RT
T15409 3907-3913 NN denotes Enzyme
T15410 3914-3922 NN denotes activity
T15411 3923-3926 VB denotes was
T15412 3927-3937 VB denotes visualised
T15413 3938-3940 IN denotes by
T15414 3941-3944 DT denotes the
T15415 3945-3953 VB denotes enhanced
T15416 3954-3971 NN denotes chemiluminescence
T15417 3972-3978 NN denotes system
T15418 3978-3979 -COMMA- denotes ,
T15419 3980-3988 NN denotes ECL+PLUS
T15420 3989-3990 -LRB- denotes (
T15421 3990-3998 NNP denotes Amersham
T15422 3998-3999 -COMMA- denotes ,
T15423 4000-4015 NNP denotes Buckinghamshire
T15424 4015-4016 -COMMA- denotes ,
T15425 4017-4019 NNP denotes UK
T15426 4019-4020 -RRB- denotes )
T15427 4022-4035 JJ denotes Densitometric
T15428 4036-4044 NN denotes analysis
T15429 4045-4048 VB denotes was
T15430 4049-4058 VB denotes performed
T15431 4059-4061 IN denotes by
T15432 4062-4070 VB denotes scanning
T15433 4071-4081 NN denotes hyperfilms
T15434 4082-4084 IN denotes on
T15435 4085-4086 DT denotes a
T15436 4087-4095 NNP denotes Personal
T15437 4096-4108 NNP denotes Densitometer
T15438 4109-4111 NNP denotes SI
T15439 4112-4113 -LRB- denotes (
T15440 4113-4122 NNP denotes Molecular
T15441 4123-4131 NNP denotes Dynamics
T15442 4131-4132 -COMMA- denotes ,
T15443 4133-4142 NNP denotes Sunnyvale
T15444 4142-4143 -COMMA- denotes ,
T15445 4144-4146 NNP denotes CA
T15446 4146-4147 -RRB- denotes )
T15447 4149-4163 NN denotes Quantification
T15448 4164-4166 IN denotes of
T15449 4167-4170 NN denotes Id1
T15450 4170-4171 -COMMA- denotes ,
T15451 4172-4175 NN denotes Id2
T15452 4176-4179 CC denotes and
T15453 4180-4183 NN denotes Id3
T15454 4184-4191 NN denotes protein
T15455 4192-4195 VB denotes was
T15456 4196-4206 VB denotes calculated
T15457 4207-4209 IN denotes by
T15458 4210-4221 VB denotes normalizing
T15459 4222-4225 DT denotes the
T15460 4226-4234 JJ denotes specific
T15461 4235-4242 NN denotes protein
T15462 4243-4248 NN denotes bands
T15463 4249-4251 TO denotes to
T15464 4252-4259 NN denotes β-actin
T15465 4260-4265 VB denotes using
T15466 4266-4271 NN denotes Image
T15467 4272-4277 JJ denotes Quant
T15468 4278-4281 CD denotes 5.5
T15469 4282-4290 NN denotes software
T15470 4291-4292 -LRB- denotes (
T15471 4292-4301 NNP denotes Molecular
T15472 4302-4310 NNP denotes Dynamics
T15473 4310-4311 -RRB- denotes )
T15608 4314-4322 NN denotes Analysis
T15609 4323-4325 IN denotes of
T15610 4326-4331 NN denotes BMP-6
T15611 4332-4341 NN denotes messenger
T15612 4342-4345 NN denotes RNA
T15613 4346-4347 -LRB- denotes (
T15614 4347-4351 NN denotes mRNA
T15615 4351-4352 -RRB- denotes )
T15616 4353-4363 NN denotes expression
T15617 4364-4374 JJ denotes Endogenous
T15618 4375-4385 NN denotes expression
T15619 4386-4388 IN denotes of
T15620 4389-4392 DT denotes the
T15621 4393-4398 NN denotes BMP-6
T15622 4399-4403 NN denotes gene
T15623 4404-4407 VB denotes was
T15624 4408-4416 VB denotes examined
T15625 4417-4419 IN denotes by
T15626 4420-4427 JJ denotes reverse
T15627 4428-4452 NN denotes transcription-polymerase
T15628 4453-4458 NN denotes chain
T15629 4459-4467 NN denotes reaction
T15630 4469-4474 JJ denotes Total
T15631 4475-4478 NN denotes RNA
T15632 4479-4482 VB denotes was
T15633 4483-4491 VB denotes isolated
T15634 4492-4497 VB denotes using
T15635 4498-4508 RB denotes Absolutely
T15636 4509-4513 NNP denotes RNA™
T15637 4514-4520 NN denotes RT-PCR
T15638 4521-4529 NNP denotes Miniprep
T15639 4530-4533 NN denotes Kit
T15640 4534-4535 -LRB- denotes (
T15641 4535-4545 NNP denotes Stratagene
T15642 4546-4552 NNP denotes Europe
T15643 4552-4553 -COMMA- denotes ,
T15644 4554-4563 NNP denotes Amsterdam
T15645 4563-4564 -COMMA- denotes ,
T15646 4565-4575 NNP denotes Netherland
T15647 4575-4576 -RRB- denotes )
T15648 4577-4586 VB denotes according
T15649 4587-4589 TO denotes to
T15650 4590-4593 DT denotes the
T15651 4594-4607 NN denotes manufacturers
T15652 4608-4620 NN denotes instructions
T15653 4622-4636 NN denotes Quantification
T15654 4637-4639 IN denotes of
T15655 4640-4643 DT denotes the
T15656 4644-4652 VB denotes isolated
T15657 4653-4658 JJ denotes total
T15658 4659-4662 NN denotes RNA
T15659 4663-4666 VB denotes was
T15660 4667-4675 VB denotes achieved
T15661 4676-4678 IN denotes by
T15662 4679-4684 VB denotes using
T15663 4685-4703 JJ denotes spectrophotometric
T15664 4704-4709 NN denotes OD260
T15665 4710-4722 NN denotes measurements
T15666 4724-4729 JJ denotes Equal
T15667 4730-4737 NN denotes amounts
T15668 4738-4740 IN denotes of
T15669 4741-4744 NN denotes RNA
T15670 4745-4749 VB denotes were
T15671 4750-4754 RB denotes then
T15672 4755-4762 VB denotes reverse
T15673 4763-4774 VB denotes transcribed
T15674 4775-4777 TO denotes to
T15675 4778-4782 NN denotes cDNA
T15676 4783-4787 IN denotes with
T15677 4788-4795 NNP denotes TaqMan®
T15678 4796-4803 NNP denotes Reverse
T15679 4804-4817 NN denotes Transcription
T15680 4818-4826 NN denotes Reagents
T15681 4827-4828 -LRB- denotes (
T15682 4828-4835 NNP denotes Applied
T15683 4836-4846 NNP denotes Biosystems
T15684 4846-4847 -COMMA- denotes ,
T15685 4848-4854 NNP denotes Foster
T15686 4855-4859 NNP denotes City
T15687 4859-4860 -COMMA- denotes ,
T15688 4861-4863 NNP denotes CA
T15689 4863-4864 -COMMA- denotes ,
T15690 4865-4870 NN denotes U.S.A
T15691 4870-4871 -RRB- denotes )
T15692 4873-4875 TO denotes To
T15693 4876-4883 VB denotes measure
T15694 4884-4888 NN denotes mRNA
T15695 4889-4899 NN denotes expression
T15696 4900-4902 IN denotes of
T15697 4903-4907 NN denotes BMP6
T15698 4907-4908 -COMMA- denotes ,
T15699 4909-4916 NN denotes Id1–Id4
T15700 4917-4920 CC denotes and
T15701 4921-4925 NN denotes PGK1
T15702 4926-4929 NN denotes PCR
T15703 4930-4934 VB denotes were
T15704 4935-4942 VB denotes carried
T15705 4943-4946 RP denotes out
T15706 4947-4951 IN denotes with
T15707 4952-4959 NNP denotes TaqMan®
T15708 4960-4969 JJ denotes universal
T15709 4970-4976 NN denotes master
T15710 4977-4980 NN denotes mix
T15711 4982-4989 NN denotes Primers
T15712 4990-4993 CC denotes and
T15713 4994-5000 NN denotes probes
T15714 5001-5005 VB denotes were
T15715 5006-5014 VB denotes provided
T15716 5015-5017 IN denotes by
T15717 5018-5033 NN denotes Assay-on-Demand
T15718 5034-5035 -LRB- denotes (
T15719 5035-5042 NNP denotes Applied
T15720 5043-5053 NNP denotes Biosystems
T15721 5053-5054 -RRB- denotes )
T15722 5056-5059 NN denotes PCR
T15723 5060-5069 NN denotes reactions
T15724 5070-5074 VB denotes were
T15725 5075-5082 VB denotes carried
T15726 5083-5086 RP denotes out
T15727 5087-5089 IN denotes in
T15728 5090-5091 DT denotes a
T15729 5092-5097 JJ denotes final
T15730 5098-5104 NN denotes volume
T15731 5105-5107 IN denotes of
T15732 5108-5110 CD denotes 25
T15733 5111-5113 NN denotes μl
T15734 5114-5115 -LRB- denotes (
T15735 5115-5120 NN denotes BMP-6
T15736 5120-5121 -RRB- denotes )
T15737 5122-5124 CC denotes or
T15738 5125-5127 CD denotes 20
T15739 5128-5130 NN denotes μl
T15740 5131-5132 -LRB- denotes (
T15741 5132-5135 NN denotes ID1
T15742 5135-5136 -RRB- denotes )
T15743 5138-5141 DT denotes The
T15744 5142-5146 NN denotes cDNA
T15745 5147-5152 VB denotes added
T15746 5153-5155 TO denotes to
T15747 5156-5160 DT denotes each
T15748 5161-5169 NN denotes reaction
T15749 5170-5173 VB denotes was
T15750 5174-5184 JJ denotes equivalent
T15751 5185-5187 TO denotes to
T15752 5188-5191 DT denotes the
T15753 5192-5197 NN denotes input
T15754 5198-5200 IN denotes of
T15755 5201-5203 CD denotes 20
T15756 5204-5206 NN denotes ng
T15757 5207-5209 IN denotes of
T15758 5210-5215 JJ denotes total
T15759 5216-5219 NN denotes RNA
T15760 5221-5224 DT denotes The
T15761 5225-5229 NN denotes gene
T15762 5230-5240 NN denotes expression
T15763 5241-5244 VB denotes was
T15764 5245-5255 VB denotes quantified
T15765 5256-5261 VB denotes using
T15766 5262-5265 DT denotes the
T15767 5266-5274 JJ denotes standard
T15768 5275-5280 NN denotes curve
T15769 5281-5287 NN denotes method
T15770 5288-5289 -LRB- denotes (
T15771 5289-5293 NN denotes BMP6
T15772 5293-5294 -RRB- denotes )
T15773 5294-5295 -COMMA- denotes ,
T15774 5296-5298 CC denotes or
T15775 5299-5302 DT denotes the
T15776 5303-5314 JJ denotes comparative
T15777 5315-5317 NN denotes CT
T15778 5318-5324 NN denotes method
T15779 5325-5326 -LRB- denotes (
T15780 5326-5329 NN denotes Id1
T15781 5329-5330 -RRB- denotes )
T15782 5331-5333 IN denotes as
T15783 5334-5343 VB denotes described
T15784 5344-5346 IN denotes in
T15785 5347-5354 NNP denotes ABI7700
T15786 5355-5359 NNP denotes User
T15787 5360-5368 NNP denotes Bulletin
T15788 5369-5370 CD denotes 2
T15789 5371-5372 -LRB- denotes (
T15790 5372-5379 NNP denotes Applied
T15791 5380-5390 NNP denotes Biosystems
T15792 5390-5391 -RRB- denotes )
T15793 5393-5396 DT denotes The
T15794 5397-5407 NN denotes expression
T15795 5408-5411 VB denotes was
T15796 5412-5416 RB denotes then
T15797 5417-5427 VB denotes normalized
T15798 5428-5430 TO denotes to
T15799 5431-5434 DT denotes the
T15800 5435-5445 NN denotes expression
T15801 5446-5451 NN denotes level
T15802 5452-5454 IN denotes of
T15803 5455-5459 NN denotes PGK1
T15804 5461-5465 NN denotes PGK1
T15805 5466-5469 VB denotes was
T15806 5470-5476 VB denotes chosen
T15807 5476-5477 -COMMA- denotes ,
T15808 5478-5485 IN denotes because
T15809 5486-5488 PRP denotes it
T15810 5489-5492 VB denotes has
T15811 5493-5497 VB denotes been
T15812 5498-5503 VB denotes shown
T15813 5504-5506 TO denotes to
T15814 5507-5511 VB denotes have
T15815 5512-5515 JJ denotes low
T15816 5516-5526 NN denotes expression
T15817 5527-5538 NN denotes variability
T15818 5539-5544 IN denotes among
T15819 5545-5555 NN denotes lymphocyte
T15820 5556-5565 NN denotes specimens
T15821 5566-5567 -LRB- denotes [
T15822 5567-5569 CD denotes 56
T15823 5569-5570 -RRB- denotes ]
T15824 5572-5582 NN denotes Expression
T15825 5583-5589 NN denotes levels
T15826 5590-5592 IN denotes in
T15827 5593-5594 NN denotes B
T15828 5595-5600 NN denotes cells
T15829 5601-5605 VB denotes were
T15830 5606-5610 RB denotes then
T15831 5611-5618 VB denotes related
T15832 5619-5621 TO denotes to
T15833 5622-5625 DT denotes the
T15834 5626-5636 NN denotes expression
T15835 5637-5643 NN denotes levels
T15836 5644-5646 IN denotes in
T15837 5647-5652 NN denotes Ramos
T15838 5653-5658 NN denotes cells
T16237 5661-5665 NN denotes Cell
T16238 5666-5679 NN denotes proliferation
T16239 5680-5683 IN denotes For
T16240 5684-5694 NN denotes estimation
T16241 5695-5697 IN denotes of
T16242 5698-5701 NN denotes DNA
T16243 5702-5711 NN denotes synthesis
T16244 5711-5712 -COMMA- denotes ,
T16245 5713-5718 JJ denotes CD19+
T16246 5719-5724 NN denotes cells
T16247 5725-5726 -LRB- denotes (
T16248 5726-5729 CD denotes 7.5
T16249 5730-5731 CC denotes ×
T16250 5732-5735 CD denotes 104
T16251 5736-5745 NN denotes cells/0.2
T16252 5746-5748 NN denotes ml
T16253 5748-5749 -RRB- denotes )
T16254 5750-5752 CC denotes or
T16255 5753-5758 NN denotes Ramos
T16256 5759-5764 NN denotes cells
T16257 5765-5766 -LRB- denotes (
T16258 5766-5767 CD denotes 1
T16259 5768-5769 NN denotes ×
T16260 5770-5773 CD denotes 104
T16261 5774-5783 NN denotes cells/0.2
T16262 5784-5786 NN denotes ml
T16263 5786-5787 -RRB- denotes )
T16264 5788-5792 VB denotes were
T16265 5793-5801 VB denotes cultured
T16266 5802-5804 IN denotes in
T16267 5805-5815 NN denotes triplicate
T16268 5816-5818 IN denotes in
T16269 5819-5829 NN denotes microtiter
T16270 5830-5835 NN denotes wells
T16271 5837-5840 DT denotes The
T16272 5841-5846 NN denotes cells
T16273 5847-5851 VB denotes were
T16274 5852-5858 VB denotes pulsed
T16275 5859-5863 IN denotes with
T16276 5864-5867 CD denotes 3.7
T16277 5868-5869 CC denotes ×
T16278 5870-5875 NN denotes 104Bq
T16279 5876-5877 -LRB- denotes [
T16280 5877-5879 NN denotes 3H
T16281 5879-5880 -RRB- denotes ]
T16282 5880-5889 NN denotes thymidine
T16283 5890-5891 -LRB- denotes (
T16284 5891-5899 NNP denotes Amersham
T16285 5899-5900 -COMMA- denotes ,
T16286 5901-5916 NNP denotes Buckinghamshire
T16287 5916-5917 -COMMA- denotes ,
T16288 5918-5920 NNP denotes UK
T16289 5920-5921 -RRB- denotes )
T16290 5922-5925 IN denotes for
T16291 5926-5929 DT denotes the
T16292 5930-5934 JJ denotes last
T16293 5935-5937 CD denotes 16
T16294 5938-5939 NN denotes h
T16295 5940-5942 IN denotes of
T16296 5943-5944 DT denotes a
T16297 5945-5949 JJ denotes 72-h
T16298 5950-5960 NN denotes incubation
T16299 5962-5965 DT denotes The
T16300 5966-5971 NN denotes cells
T16301 5972-5976 VB denotes were
T16302 5977-5986 VB denotes harvested
T16303 5987-5992 VB denotes using
T16304 5993-5995 DT denotes an
T16305 5996-6005 VB denotes automated
T16306 6006-6010 NN denotes cell
T16307 6011-6020 NN denotes harvester
T16308 6021-6022 -LRB- denotes (
T16309 6022-6029 NNP denotes Packard
T16310 6030-6040 NNP denotes Instrument
T16311 6041-6048 NNP denotes Company
T16312 6048-6049 -COMMA- denotes ,
T16313 6050-6057 NNP denotes Meriden
T16314 6057-6058 -COMMA- denotes ,
T16315 6059-6061 NNP denotes CT
T16316 6061-6062 -COMMA- denotes ,
T16317 6063-6066 NNP denotes USA
T16318 6066-6067 -RRB- denotes )
T16319 6068-6071 CC denotes and
T16320 6072-6073 -LRB- denotes [
T16321 6073-6075 NN denotes 3H
T16322 6075-6076 -RRB- denotes ]
T16323 6076-6085 NN denotes thymidine
T16324 6086-6099 NN denotes incorporation
T16325 6100-6103 VB denotes was
T16326 6104-6114 VB denotes determined
T16327 6115-6117 IN denotes in
T16328 6118-6119 DT denotes a
T16329 6120-6133 NN denotes scintillation
T16330 6134-6141 NN denotes counter
T16331 6142-6143 -LRB- denotes (
T16332 6143-6151 NNP denotes TopCount
T16333 6151-6152 -COMMA- denotes ,
T16334 6153-6160 NNP denotes Packard
T16335 6161-6171 NNP denotes Instrument
T16336 6172-6179 NNP denotes Company
T16337 6180-6184 NNP denotes Inc.
T16338 6184-6185 -COMMA- denotes ,
T16339 6186-6193 NNP denotes Meriden
T16340 6193-6194 -COMMA- denotes ,
T16341 6195-6197 NN denotes CT
T16342 6197-6198 -RRB- denotes )
T16481 6201-6214 NN denotes Determination
T16482 6215-6217 IN denotes of
T16483 6218-6222 NN denotes cell
T16484 6223-6228 NN denotes death
T16485 6229-6233 NN denotes Cell
T16486 6234-6239 NN denotes death
T16487 6240-6243 VB denotes was
T16488 6244-6252 VB denotes measured
T16489 6253-6255 IN denotes by
T16490 6256-6261 JJ denotes vital
T16491 6262-6265 NN denotes dye
T16492 6266-6275 NN denotes exclusion
T16493 6276-6280 NN denotes test
T16494 6281-6283 IN denotes by
T16495 6284-6292 VB denotes staining
T16496 6293-6298 NN denotes cells
T16497 6299-6303 IN denotes with
T16498 6304-6305 CD denotes 5
T16499 6306-6311 NN denotes μg/ml
T16500 6312-6321 NN denotes propidium
T16501 6322-6328 NN denotes iodide
T16502 6329-6330 -LRB- denotes (
T16503 6330-6331 -LRB- denotes [
T16504 6331-6333 NNP denotes PI
T16505 6333-6334 -RRB- denotes ]
T16506 6334-6335 -COLON- denotes ;
T16507 6336-6346 NNP denotes Calbiochem
T16508 6347-6352 NNP denotes Corp.
T16509 6352-6353 -COLON- denotes ;
T16510 6354-6356 NNP denotes La
T16511 6357-6362 NNP denotes Jolla
T16512 6362-6363 -COMMA- denotes ,
T16513 6364-6366 NNP denotes CA
T16514 6366-6367 -COLON- denotes ;
T16515 6368-6369 CD denotes 5
T16516 6370-6375 NN denotes mg/ml
T16517 6375-6376 -RRB- denotes )
T16518 6377-6380 IN denotes for
T16519 6381-6384 CD denotes one
T16520 6385-6391 NN denotes minute
T16521 6392-6394 IN denotes on
T16522 6395-6398 NN denotes ice
T16523 6400-6402 IN denotes At
T16524 6403-6408 JJ denotes least
T16525 6409-6414 CD denotes 1,000
T16526 6415-6420 NN denotes cells
T16527 6421-6424 IN denotes per
T16528 6425-6431 NN denotes sample
T16529 6432-6436 VB denotes were
T16530 6437-6440 VB denotes run
T16531 6441-6443 IN denotes on
T16532 6444-6445 DT denotes a
T16533 6446-6448 NN denotes BD
T16534 6449-6460 NN denotes FACSCalibur
T16535 6461-6465 NN denotes flow
T16536 6466-6475 NN denotes cytometer
T16609 6478-6489 JJ denotes Statistical
T16610 6490-6498 NN denotes analysis
T16611 6499-6502 DT denotes The
T16612 6503-6514 JJ denotes statistical
T16613 6515-6527 NN denotes significance
T16614 6528-6530 IN denotes of
T16615 6531-6542 NN denotes differences
T16616 6543-6550 IN denotes between
T16617 6551-6557 NN denotes groups
T16618 6558-6561 VB denotes was
T16619 6562-6572 VB denotes determined
T16620 6573-6578 VB denotes using
T16621 6579-6582 DT denotes the
T16622 6583-6589 JJ denotes paired
T16623 6590-6600 JJ denotes two-tailed
T16624 6601-6609 NNP denotes Wilcoxon
T16625 6610-6623 JJ denotes nonparametric
T16626 6624-6628 NN denotes test
T16627 6628-6629 -COMMA- denotes ,
T16628 6630-6632 IN denotes by
T16629 6633-6641 VB denotes applying
T16630 6642-6650 NN denotes SPSS10.1
T16631 6651-6659 NN denotes software
T16632 6660-6661 -LRB- denotes (
T16633 6661-6665 NNP denotes SPSS
T16634 6666-6670 NNP denotes Inc.
T16635 6670-6671 -COMMA- denotes ,
T16636 6672-6679 NNP denotes Chicago
T16637 6679-6680 -COMMA- denotes ,
T16638 6681-6683 NNP denotes IL
T16639 6683-6684 -COMMA- denotes ,
T16640 6685-6688 NNP denotes USA
T16641 6688-6689 -RRB- denotes )
T16642 6691-6692 NN denotes P
T16643 6693-6699 NN denotes values
T16644 6700-6704 JJ denotes less
T16645 6705-6709 IN denotes than
T16646 6710-6714 CD denotes 0.05
T16647 6715-6719 VB denotes were
T16648 6720-6730 VB denotes considered
T16649 6731-6742 JJ denotes significant
R10633 T13709 T13708 arg1Of culture,Cell
R10634 T13712 T13710 arg2Of specified,If
R10635 T13712 T13711 arg1Of specified,not
R10636 T13715 T13712 arg2Of cells,specified
R10637 T13715 T13714 arg1Of cells,all
R10638 T13715 T13716 arg1Of cells,were
R10639 T13715 T13717 arg2Of cells,cultured
R10640 T13717 T13710 arg1Of cultured,If
R10641 T13717 T13713 arg1Of cultured,","
R10642 T13717 T13716 arg2Of cultured,were
R10643 T13717 T13718 arg1Of cultured,in
R10644 T13719 T13720 arg1Of X-VIVO,15™
R10645 T13719 T13721 arg1Of X-VIVO,(
R10646 T13722 T13723 arg1Of BioWhittaker,","
R10647 T13723 T13721 arg2Of ",",(
R10648 T13723 T13725 arg1Of ",",","
R10649 T13723 T13726 arg1Of ",",Belgium
R10650 T13724 T13723 arg2Of Verviers,","
R10651 T13727 T13721 arg3Of ),(
R10652 T13729 T13718 arg2Of medium,in
R10653 T13729 T13719 arg1Of medium,X-VIVO
R10654 T13729 T13728 arg1Of medium,serum-free
R10655 T13729 T13730 arg1Of medium,at
R10656 T13731 T13732 arg1Of 37°C,and
R10657 T13732 T13730 arg2Of and,at
R10658 T13732 T13736 arg1Of and,in
R10659 T13733 T13734 arg1Of 5,%
R10660 T13735 T13732 arg2Of CO2,and
R10661 T13735 T13733 arg1Of CO2,5
R10662 T13737 T13736 arg2Of air,in
R10663 T13739 T13738 arg1Of blood,Peripheral
R10664 T13739 T13740 arg1Of blood,was
R10665 T13739 T13741 arg2Of blood,provided
R10666 T13741 T13740 arg2Of provided,was
R10667 T13741 T13750 arg1Of provided,with
R10668 T13745 T13741 arg1Of Bank,provided
R10669 T13745 T13742 arg2Of Bank,by
R10670 T13745 T13743 arg1Of Bank,the
R10671 T13745 T13744 arg1Of Bank,Blood
R10672 T13745 T13746 arg1Of Bank,at
R10673 T13749 T13746 arg2Of Hospital,at
R10674 T13749 T13747 arg1Of Hospital,Buskerud
R10675 T13749 T13748 arg1Of Hospital,Regional
R10676 T13752 T13750 arg2Of agreement,with
R10677 T13752 T13751 arg1Of agreement,formal
R10678 T13752 T13753 arg1Of agreement,by
R10679 T13755 T13757 arg1Of patients,and
R10680 T13757 T13753 arg2Of and,by
R10681 T13757 T13754 arg1Of and,the
R10682 T13757 T13756 arg1Of and,","
R10683 T13757 T13759 arg1Of and,by
R10684 T13758 T13757 arg2Of approval,and
R10685 T13763 T13759 arg2Of committee,by
R10686 T13763 T13760 arg1Of committee,the
R10687 T13763 T13761 arg1Of committee,regional
R10688 T13763 T13762 arg1Of committee,ethics
R10689 T13765 T13764 arg1Of purified,Highly
R10690 T13768 T13765 arg1Of B-lymphocytes,purified
R10691 T13768 T13766 arg1Of B-lymphocytes,resting
R10692 T13768 T13767 arg1Of B-lymphocytes,human
R10693 T13768 T13769 arg1Of B-lymphocytes,(
R10694 T13768 T13773 arg1Of B-lymphocytes,were
R10695 T13768 T13774 arg2Of B-lymphocytes,isolated
R10696 T13768 T13780 arg1Of B-lymphocytes,rosetting
R10697 T13771 T13769 arg2Of cells,(
R10698 T13771 T13770 arg1Of cells,CD19+
R10699 T13772 T13769 arg3Of ),(
R10700 T13774 T13773 arg2Of isolated,were
R10701 T13774 T13775 arg1Of isolated,from
R10702 T13774 T13779 arg1Of isolated,by
R10703 T13778 T13775 arg2Of blood,from
R10704 T13778 T13776 arg1Of blood,the
R10705 T13778 T13777 arg1Of blood,peripheral
R10706 T13780 T13779 arg2Of rosetting,by
R10707 T13780 T13781 arg1Of rosetting,with
R10708 T13780 T13794 arg1Of rosetting,as
R10709 T13780 T13796 arg1Of rosetting,[
R10710 T13783 T13781 arg2Of beads,with
R10711 T13783 T13782 arg1Of beads,immunomagnetic
R10712 T13783 T13784 arg1Of beads,(
R10713 T13786 T13787 arg1Of M450,;
R10714 T13787 T13784 arg2Of ;,(
R10715 T13787 T13785 arg1Of ;,Dynabeads
R10716 T13787 T13791 arg1Of ;,","
R10717 T13787 T13792 arg1Of ;,Norway
R10718 T13788 T13789 arg1Of Dynal,","
R10719 T13789 T13787 arg2Of ",",;
R10720 T13790 T13789 arg2Of Oslo,","
R10721 T13793 T13784 arg3Of ),(
R10722 T13795 T13794 arg2Of described,as
R10723 T13797 T13796 arg2Of 55,[
R10724 T13798 T13796 arg3Of ],[
R10725 T13800 T13799 arg1Of procedure,This
R10726 T13800 T13801 arg1Of procedure,yields
R10727 T13801 T13818 arg1Of yields,as
R10728 T13802 T13803 arg1Of less,than
R10729 T13802 T13805 arg1Of less,%
R10730 T13804 T13803 arg2Of 0.5,than
R10731 T13807 T13802 arg1Of cells,less
R10732 T13807 T13806 arg1Of cells,T
R10733 T13807 T13808 arg1Of cells,","
R10734 T13808 T13814 arg1Of ",",and
R10735 T13809 T13810 arg1Of 0.1,%
R10736 T13812 T13808 arg2Of cells,","
R10737 T13812 T13809 arg1Of cells,0.1
R10738 T13812 T13811 arg1Of cells,NK
R10739 T13814 T13801 arg2Of and,yields
R10740 T13814 T13813 arg1Of and,","
R10741 T13815 T13816 arg1Of 0.5,%
R10742 T13817 T13814 arg2Of monocytes,and
R10743 T13817 T13815 arg1Of monocytes,0.5
R10744 T13819 T13818 arg2Of judged,as
R10745 T13823 T13819 arg1Of staining,judged
R10746 T13823 T13820 arg2Of staining,by
R10747 T13823 T13821 arg1Of staining,indirect
R10748 T13823 T13822 arg1Of staining,immunofluorescence
R10749 T13827 T13824 arg1Of lines,The
R10750 T13827 T13825 arg1Of lines,following
R10751 T13827 T13826 arg1Of lines,cell
R10752 T13827 T13828 arg1Of lines,from
R10753 T13827 T13832 arg1Of lines,were
R10754 T13827 T13833 arg2Of lines,maintained
R10755 T13827 T13870 arg1Of lines,serum-starved
R10756 T13827 T13877 arg2Of lines,cultured
R10757 T13827 T13882 arg1Of lines,included
R10758 T13831 T13828 arg2Of malignancies,from
R10759 T13831 T13829 arg1Of malignancies,human
R10760 T13831 T13830 arg1Of malignancies,lymphoid
R10761 T13833 T13834 arg1Of maintained,in
R10762 T13833 T13869 arg1Of maintained,but
R10763 T13835 T13834 arg2Of RPMI,in
R10764 T13835 T13836 arg1Of RPMI,1640
R10765 T13835 T13837 arg1Of RPMI,(
R10766 T13835 T13846 arg2Of RPMI,supplemented
R10767 T13840 T13837 arg2Of GmbH,(
R10768 T13840 T13838 arg1Of GmbH,PAA
R10769 T13840 T13839 arg1Of GmbH,Laboratories
R10770 T13840 T13841 arg1Of GmbH,","
R10771 T13842 T13841 arg2Of Pasching,","
R10772 T13842 T13843 arg1Of Pasching,","
R10773 T13842 T13844 arg1Of Pasching,Austria
R10774 T13845 T13837 arg3Of ),(
R10775 T13846 T13847 arg1Of supplemented,with
R10776 T13848 T13849 arg1Of 10,%
R10777 T13852 T13848 arg1Of serum,10
R10778 T13852 T13850 arg1Of serum,foetal
R10779 T13852 T13851 arg1Of serum,bovine
R10780 T13852 T13853 arg1Of serum,(
R10781 T13852 T13856 arg1Of serum,","
R10782 T13854 T13853 arg2Of FCS,(
R10783 T13855 T13853 arg3Of ),(
R10784 T13856 T13862 arg1Of ",",and
R10785 T13860 T13856 arg2Of G,","
R10786 T13860 T13857 arg1Of G,100
R10787 T13860 T13858 arg1Of G,units/ml
R10788 T13860 T13859 arg1Of G,penicillin
R10789 T13862 T13847 arg2Of and,with
R10790 T13862 T13861 arg1Of and,","
R10791 T13864 T13862 arg2Of units/ml,and
R10792 T13864 T13863 arg1Of units/ml,100
R10793 T13864 T13865 arg1Of units/ml,of
R10794 T13867 T13865 arg2Of sulphate,of
R10795 T13867 T13866 arg1Of sulphate,streptomycin
R10796 T13869 T13868 arg1Of but,","
R10797 T13869 T13871 arg1Of but,for
R10798 T13869 T13876 arg1Of but,and
R10799 T13870 T13869 arg2Of serum-starved,but
R10800 T13874 T13872 arg1Of four,at
R10801 T13874 T13873 arg1Of four,least
R10802 T13875 T13871 arg2Of hours,for
R10803 T13875 T13874 arg1Of hours,four
R10804 T13876 T13832 arg2Of and,were
R10805 T13876 T13881 arg1Of and,when
R10806 T13877 T13876 arg2Of cultured,and
R10807 T13877 T13878 arg1Of cultured,in
R10808 T13879 T13878 arg2Of X-VIVO,in
R10809 T13879 T13880 arg1Of X-VIVO,15™
R10810 T13882 T13881 arg2Of included,when
R10811 T13882 T13883 arg1Of included,in
R10812 T13884 T13883 arg2Of experiments,in
R10813 T13884 T13885 arg1Of experiments,:
R10814 T13890 T13886 arg1Of Ramos,EBV-negative
R10815 T13890 T13887 arg1Of Ramos,BL
R10816 T13890 T13888 arg1Of Ramos,cell
R10817 T13890 T13889 arg1Of Ramos,lines
R10818 T13890 T13891 arg1Of Ramos,(
R10819 T13890 T13895 arg1Of Ramos,","
R10820 T13892 T13891 arg2Of ECACC,(
R10821 T13892 T13893 arg1Of ECACC,85030802
R10822 T13894 T13891 arg3Of ),(
R10823 T13895 T13882 arg2Of ",",included
R10824 T13896 T13895 arg2Of HL60,","
R10825 T13896 T13897 arg1Of HL60,(
R10826 T13898 T13897 arg2Of JCRB0085,(
R10827 T13899 T13897 arg3Of ),(
R11164 T14428 T14429 arg2Of Antibodies,used
R11165 T14429 T14430 arg1Of used,for
R11166 T14433 T14431 arg1Of analysis,flow
R11167 T14433 T14432 arg1Of analysis,cytometric
R11168 T14433 T14434 arg1Of analysis,and
R11169 T14434 T14430 arg2Of and,for
R11170 T14436 T14434 arg2Of analysis,and
R11171 T14436 T14435 arg1Of analysis,immunoblot
R11172 T14437 T14438 arg1Of Antibodies,against
R11173 T14437 T14451 arg1Of Antibodies,were
R11174 T14437 T14452 arg2Of Antibodies,purchased
R11175 T14441 T14439 arg1Of BMP-receptors,the
R11176 T14441 T14440 arg1Of BMP-receptors,human
R11177 T14442 T14443 arg1Of Act-RIA,","
R11178 T14443 T14445 arg1Of ",",","
R11179 T14444 T14443 arg2Of BMP-RIB,","
R11180 T14445 T14447 arg1Of ",",","
R11181 T14446 T14445 arg2Of BMPR-II,","
R11182 T14447 T14449 arg1Of ",",and
R11183 T14448 T14447 arg2Of Act-RIIA,","
R11184 T14449 T14438 arg2Of and,against
R11185 T14449 T14441 arg1Of and,BMP-receptors
R11186 T14450 T14449 arg2Of Act-RIIb,and
R11187 T14452 T14451 arg2Of purchased,were
R11188 T14452 T14453 arg1Of purchased,from
R11189 T14457 T14453 arg2Of Systems,from
R11190 T14457 T14454 arg1Of Systems,R
R11191 T14457 T14455 arg1Of Systems,&
R11192 T14457 T14456 arg1Of Systems,D
R11193 T14457 T14458 arg1Of Systems,(
R11194 T14459 T14458 arg2Of Abingdon,(
R11195 T14459 T14460 arg1Of Abingdon,","
R11196 T14459 T14461 arg1Of Abingdon,UK
R11197 T14462 T14458 arg3Of ),(
R11198 T14463 T14464 arg1Of Detection,of
R11199 T14463 T14467 arg1Of Detection,has
R11200 T14463 T14468 arg1Of Detection,been
R11201 T14463 T14469 arg2Of Detection,tried
R11202 T14466 T14464 arg2Of BMP-6-protein,of
R11203 T14466 T14465 arg1Of BMP-6-protein,the
R11204 T14469 T14467 arg2Of tried,has
R11205 T14469 T14468 arg2Of tried,been
R11206 T14469 T14470 arg1Of tried,with
R11207 T14473 T14470 arg2Of antibodies,with
R11208 T14473 T14471 arg1Of antibodies,the
R11209 T14473 T14472 arg1Of antibodies,following
R11210 T14473 T14474 arg1Of antibodies,:
R11211 T14477 T14475 arg1Of anti-BMP-6,goat
R11212 T14477 T14476 arg1Of anti-BMP-6,polyclonal
R11213 T14477 T14478 arg1Of anti-BMP-6,(
R11214 T14477 T14489 arg1Of anti-BMP-6,","
R11215 T14480 T14478 arg2Of Cruz,(
R11216 T14480 T14479 arg1Of Cruz,Santa
R11217 T14480 T14481 arg1Of Cruz,","
R11218 T14480 T14486 arg1Of Cruz,","
R11219 T14480 T14487 arg1Of Cruz,USA
R11220 T14483 T14481 arg2Of Diego,","
R11221 T14483 T14482 arg1Of Diego,San
R11222 T14483 T14484 arg1Of Diego,","
R11223 T14485 T14484 arg2Of CA,","
R11224 T14488 T14478 arg3Of ),(
R11225 T14489 T14474 arg2Of ",",:
R11226 T14489 T14497 arg1Of ",",from
R11227 T14492 T14490 arg1Of anti-BMP-6,monoclonal
R11228 T14492 T14491 arg1Of anti-BMP-6,mouse
R11229 T14492 T14493 arg1Of anti-BMP-6,and
R11230 T14493 T14489 arg2Of and,","
R11231 T14496 T14493 arg2Of anti-BMP-6,and
R11232 T14496 T14494 arg1Of anti-BMP-6,polyclonal
R11233 T14496 T14495 arg1Of anti-BMP-6,goat
R11234 T14501 T14498 arg1Of Systems,R
R11235 T14501 T14499 arg1Of Systems,&
R11236 T14501 T14500 arg1Of Systems,D
R11237 T14501 T14502 arg1Of Systems,(
R11238 T14501 T14508 arg1Of Systems,and
R11239 T14503 T14502 arg2Of Abingdon,(
R11240 T14503 T14504 arg1Of Abingdon,","
R11241 T14503 T14505 arg1Of Abingdon,UK
R11242 T14506 T14502 arg3Of ),(
R11243 T14508 T14497 arg2Of and,from
R11244 T14508 T14507 arg1Of and,","
R11245 T14511 T14508 arg2Of anti-BMP-6,and
R11246 T14511 T14509 arg1Of anti-BMP-6,mouse
R11247 T14511 T14510 arg1Of anti-BMP-6,monoclonal
R11248 T14511 T14512 arg1Of anti-BMP-6,(
R11249 T14515 T14512 arg2Of Inc,(
R11250 T14515 T14513 arg1Of Inc,Chemicon
R11251 T14515 T14514 arg1Of Inc,International
R11252 T14515 T14516 arg1Of Inc,","
R11253 T14515 T14520 arg1Of Inc,","
R11254 T14515 T14521 arg1Of Inc,USA
R11255 T14517 T14518 arg1Of Temecula,","
R11256 T14518 T14516 arg2Of ",",","
R11257 T14519 T14518 arg2Of CA,","
R11258 T14522 T14512 arg3Of ),(
R11259 T14523 T14524 arg1Of Characterisation,of
R11260 T14523 T14527 arg1Of Characterisation,was
R11261 T14523 T14528 arg2Of Characterisation,done
R11262 T14526 T14524 arg2Of pathways,of
R11263 T14526 T14525 arg1Of pathways,BMP-signalling
R11264 T14528 T14527 arg2Of done,was
R11265 T14528 T14540 arg1Of done,(
R11266 T14530 T14528 arg1Of use,done
R11267 T14530 T14529 arg2Of use,by
R11268 T14530 T14531 arg1Of use,of
R11269 T14532 T14531 arg2Of anti-phospho-Smad1,of
R11270 T14532 T14533 arg1Of anti-phospho-Smad1,","
R11271 T14532 T14535 arg1Of anti-phospho-Smad1,","
R11272 T14534 T14533 arg2Of -5,","
R11273 T14538 T14536 arg1Of polyclonal,-and
R11274 T14538 T14537 arg1Of polyclonal,8
R11275 T14539 T14535 arg2Of antibody,","
R11276 T14539 T14538 arg1Of antibody,polyclonal
R11277 T14541 T14542 arg1Of Chemicon,","
R11278 T14542 T14544 arg1Of ",",","
R11279 T14543 T14542 arg2Of Temecula,","
R11280 T14544 T14540 arg2Of ",",(
R11281 T14544 T14546 arg1Of ",",","
R11282 T14544 T14547 arg1Of ",",USA
R11283 T14545 T14544 arg2Of CA,","
R11284 T14548 T14540 arg3Of ),(
R11285 T14550 T14549 arg1Of levels,Expression
R11286 T14550 T14551 arg1Of levels,of
R11287 T14550 T14554 arg1Of levels,were
R11288 T14550 T14555 arg2Of levels,detected
R11289 T14553 T14551 arg2Of proteins,of
R11290 T14553 T14552 arg1Of proteins,Id1–3
R11291 T14555 T14554 arg2Of detected,were
R11292 T14555 T14556 arg1Of detected,with
R11293 T14555 T14560 arg1Of detected,and
R11294 T14559 T14556 arg2Of antibody,with
R11295 T14559 T14557 arg1Of antibody,polyclonal
R11296 T14559 T14558 arg1Of antibody,rabbit
R11297 T14561 T14562 arg1Of detection,was
R11298 T14561 T14563 arg2Of detection,blocked
R11299 T14563 T14560 arg2Of blocked,and
R11300 T14563 T14562 arg2Of blocked,was
R11301 T14563 T14564 arg1Of blocked,with
R11302 T14565 T14564 arg2Of blocking,with
R11303 T14566 T14565 arg2Of peptide,blocking
R11304 T14566 T14567 arg1Of peptide,from
R11305 T14570 T14567 arg2Of Biotechnology,from
R11306 T14570 T14568 arg1Of Biotechnology,Santa
R11307 T14570 T14569 arg1Of Biotechnology,Cruz
R11308 T14570 T14571 arg1Of Biotechnology,(
R11309 T14573 T14571 arg2Of Cruz,(
R11310 T14573 T14572 arg1Of Cruz,Santa
R11311 T14573 T14574 arg1Of Cruz,","
R11312 T14573 T14579 arg1Of Cruz,","
R11313 T14576 T14574 arg2Of Diego,","
R11314 T14576 T14575 arg1Of Diego,San
R11315 T14576 T14577 arg1Of Diego,","
R11316 T14578 T14577 arg2Of CA,","
R11317 T14580 T14579 arg2Of USA,","
R11318 T14581 T14571 arg3Of ),(
R11319 T14584 T14582 arg1Of antibodies,As
R11320 T14584 T14583 arg1Of antibodies,secondary
R11321 T14584 T14585 arg1Of antibodies,served
R11322 T14586 T14587 arg1Of anti-mouse,","
R11323 T14587 T14589 arg1Of ",",or
R11324 T14588 T14587 arg2Of anti-goat,","
R11325 T14590 T14589 arg2Of anti-rabbit,or
R11326 T14593 T14585 arg2Of peroxidase,served
R11327 T14593 T14586 arg1Of peroxidase,anti-mouse
R11328 T14593 T14588 arg1Of peroxidase,anti-goat
R11329 T14593 T14590 arg1Of peroxidase,anti-rabbit
R11330 T14593 T14591 arg1Of peroxidase,IgG-
R11331 T14593 T14592 arg1Of peroxidase,horseradish
R11332 T14593 T14594 arg1Of peroxidase,(
R11333 T14593 T14597 arg1Of peroxidase,from
R11334 T14593 T14600 arg1Of peroxidase,(
R11335 T14593 T14605 arg1Of peroxidase,for
R11336 T14595 T14594 arg2Of HRP,(
R11337 T14596 T14594 arg3Of ),(
R11338 T14599 T14597 arg2Of AS,from
R11339 T14599 T14598 arg1Of AS,Dakocytomation
R11340 T14601 T14600 arg2Of Copenhagen,(
R11341 T14601 T14602 arg1Of Copenhagen,","
R11342 T14603 T14602 arg2Of Denmark,","
R11343 T14604 T14600 arg3Of ),(
R11344 T14607 T14605 arg2Of analysis,for
R11345 T14607 T14606 arg1Of analysis,immunoblot
R11346 T14608 T14609 arg1Of Anti-β-actin,was
R11347 T14608 T14610 arg1Of Anti-β-actin,from
R11348 T14609 T14626 arg1Of was,anti-CD19-FITC
R11349 T14610 T14609 arg2Of from,was
R11350 T14614 T14610 arg2Of Dickinson,from
R11351 T14614 T14611 arg1Of Dickinson,Santa-Cruz.
R11352 T14614 T14612 arg1Of Dickinson,From
R11353 T14614 T14613 arg1Of Dickinson,Becton
R11354 T14614 T14615 arg1Of Dickinson,(
R11355 T14614 T14623 arg2Of Dickinson,purchased
R11356 T14617 T14615 arg2Of Jose,(
R11357 T14617 T14616 arg1Of Jose,San
R11358 T14617 T14618 arg1Of Jose,","
R11359 T14619 T14618 arg2Of CA,","
R11360 T14620 T14615 arg3Of ),(
R11361 T14622 T14623 arg1Of we,purchased
R11362 T14623 T14621 arg1Of purchased,","
R11363 T14626 T14624 arg1Of anti-CD19-FITC,anti-CD19-PE
R11364 T14626 T14625 arg1Of anti-CD19-FITC,","
R11365 T14628 T14627 arg1Of antibodies,The
R11366 T14628 T14629 arg2Of antibodies,used
R11367 T14628 T14633 arg1Of antibodies,were
R11368 T14629 T14630 arg1Of used,for
R11369 T14632 T14630 arg2Of sorting,for
R11370 T14632 T14631 arg1Of sorting,cell
R11371 T14635 T14634 arg1Of PC5,anti-CD19
R11372 T14635 T14636 arg1Of PC5,from
R11373 T14635 T14639 arg1Of PC5,(
R11374 T14635 T14644 arg1Of PC5,and
R11375 T14638 T14636 arg2Of SA,from
R11376 T14638 T14637 arg1Of SA,Immunotech
R11377 T14640 T14639 arg2Of Marseille,(
R11378 T14640 T14641 arg1Of Marseille,","
R11379 T14642 T14641 arg2Of France,","
R11380 T14643 T14639 arg3Of ),(
R11381 T14644 T14633 arg2Of and,were
R11382 T14646 T14644 arg2Of PE,and
R11383 T14646 T14645 arg1Of PE,anti-CD27
R11384 T14646 T14647 arg1Of PE,from
R11385 T14649 T14647 arg2Of Dickinson,from
R11386 T14649 T14648 arg1Of Dickinson,Becton
R11387 T14649 T14650 arg1Of Dickinson,","
R11388 T14652 T14650 arg2Of Pharmingen,","
R11389 T14652 T14651 arg1Of Pharmingen,Biosciences
R11390 T14652 T14653 arg1Of Pharmingen,(
R11391 T14655 T14653 arg2Of Diego,(
R11392 T14655 T14654 arg1Of Diego,San
R11393 T14655 T14656 arg1Of Diego,","
R11394 T14657 T14656 arg2Of CA,","
R11395 T14657 T14658 arg1Of CA,","
R11396 T14659 T14658 arg2Of USA,","
R11397 T14660 T14653 arg3Of ),(
R11658 T15103 T15102 arg1Of sorting,Cell
R11659 T15105 T15104 arg1Of purified,Highly
R11660 T15106 T15107 arg1Of CD19+CD27-,or
R11661 T15108 T15107 arg2Of CD19+CD27+,or
R11662 T15109 T15105 arg1Of cells,purified
R11663 T15109 T15106 arg1Of cells,CD19+CD27-
R11664 T15109 T15108 arg1Of cells,CD19+CD27+
R11665 T15109 T15110 arg1Of cells,were
R11666 T15109 T15111 arg2Of cells,obtained
R11667 T15111 T15110 arg2Of obtained,were
R11668 T15111 T15112 arg1Of obtained,by
R11669 T15111 T15128 arg1Of obtained,","
R11670 T15111 T15129 modOf obtained,followed
R11671 T15113 T15112 arg2Of staining,by
R11672 T15113 T15116 arg1Of staining,with
R11673 T15115 T15113 arg2Of cells,staining
R11674 T15115 T15114 arg1Of cells,CD19+
R11675 T15118 T15119 arg1Of PE,and
R11676 T15120 T15119 arg2Of CD19,and
R11677 T15122 T15116 arg2Of mAbs,with
R11678 T15122 T15117 arg1Of mAbs,anti-CD27
R11679 T15122 T15118 arg1Of mAbs,PE
R11680 T15122 T15120 arg1Of mAbs,CD19
R11681 T15122 T15121 arg1Of mAbs,PC5
R11682 T15122 T15123 arg1Of mAbs,for
R11683 T15125 T15123 arg2Of minutes,for
R11684 T15125 T15124 arg1Of minutes,30
R11685 T15125 T15126 arg1Of minutes,at
R11686 T15127 T15126 arg2Of 4°C,at
R11687 T15129 T15130 arg1Of followed,by
R11688 T15131 T15132 arg1Of washing,with
R11689 T15131 T15134 arg1Of washing,and
R11690 T15133 T15132 arg2Of PBS,with
R11691 T15134 T15130 arg2Of and,by
R11692 T15135 T15134 arg2Of sorting,and
R11693 T15135 T15136 arg1Of sorting,on
R11694 T15138 T15136 arg2Of DiVa,on
R11695 T15138 T15137 arg1Of DiVa,FACS
R11696 T15138 T15139 arg1Of DiVa,from
R11697 T15141 T15139 arg2Of Dickinson,from
R11698 T15141 T15140 arg1Of Dickinson,Becton
R11752 T15270 T15269 arg1Of analysis,Western-blot
R11753 T15272 T15271 arg1Of cells,B
R11754 T15272 T15273 arg1Of cells,from
R11755 T15272 T15279 arg1Of cells,were
R11756 T15272 T15280 arg2Of cells,lysed
R11757 T15275 T15274 arg1Of blood,peripheral
R11758 T15275 T15276 arg1Of blood,or
R11759 T15276 T15273 arg2Of or,from
R11760 T15278 T15276 arg2Of cell-lines,or
R11761 T15278 T15277 arg2Of cell-lines,cultured
R11762 T15280 T15279 arg2Of lysed,were
R11763 T15280 T15281 arg1Of lysed,in
R11764 T15283 T15281 arg2Of buffer,in
R11765 T15283 T15282 arg1Of buffer,lysis
R11766 T15283 T15284 arg1Of buffer,(
R11767 T15287 T15285 arg1Of %,glycerol
R11768 T15287 T15286 arg1Of %,10
R11769 T15287 T15288 arg1Of %,","
R11770 T15288 T15292 arg1Of ",",","
R11771 T15291 T15288 arg2Of %,","
R11772 T15291 T15289 arg1Of %,β-mercaptoethanol
R11773 T15291 T15290 arg1Of %,5
R11774 T15292 T15300 arg1Of ",",","
R11775 T15293 T15294 arg1Of 0.0625,M
R11776 T15295 T15292 arg2Of Tris-HCL,","
R11777 T15295 T15293 arg1Of Tris-HCL,0.0625
R11778 T15295 T15296 arg1Of Tris-HCL,[
R11779 T15297 T15296 arg2Of pH,[
R11780 T15297 T15298 arg1Of pH,6.8
R11781 T15299 T15296 arg3Of ],[
R11782 T15300 T15284 arg2Of ",",(
R11783 T15305 T15304 arg2Of SDS,[
R11784 T15306 T15304 arg3Of ],[
R11785 T15307 T15308 arg1Of 2.5,%
R11786 T15309 T15300 arg2Of w/vol,","
R11787 T15309 T15301 arg1Of w/vol,sodium
R11788 T15309 T15302 arg1Of w/vol,dodecyl
R11789 T15309 T15303 arg1Of w/vol,sulphate
R11790 T15309 T15304 arg1Of w/vol,[
R11791 T15309 T15307 arg1Of w/vol,2.5
R11792 T15310 T15284 arg3Of ),(
R11793 T15312 T15311 arg1Of protein,Total
R11794 T15312 T15313 arg1Of protein,(
R11795 T15312 T15317 arg1Of protein,from
R11796 T15312 T15320 arg1Of protein,was
R11797 T15312 T15321 arg2Of protein,run
R11798 T15312 T15334 arg1Of protein,blotted
R11799 T15315 T15313 arg2Of μg,(
R11800 T15315 T15314 arg1Of μg,30–100
R11801 T15316 T15313 arg3Of ),(
R11802 T15319 T15317 arg2Of sample,from
R11803 T15319 T15318 arg1Of sample,each
R11804 T15321 T15320 arg2Of run,was
R11805 T15321 T15322 arg1Of run,on
R11806 T15321 T15333 arg1Of run,and
R11807 T15324 T15323 arg1Of %,10
R11808 T15324 T15325 arg1Of %,or
R11809 T15325 T15327 arg1Of or,%
R11810 T15326 T15325 arg2Of 12,or
R11811 T15330 T15329 arg2Of SDS/PAGE,(
R11812 T15331 T15329 arg3Of ),(
R11813 T15332 T15322 arg2Of gels,on
R11814 T15332 T15324 arg1Of gels,%
R11815 T15332 T15326 arg1Of gels,12
R11816 T15332 T15328 arg1Of gels,SDS/polyacrylamide
R11817 T15332 T15329 arg1Of gels,(
R11818 T15333 T15338 arg1Of and,(
R11819 T15334 T15333 arg2Of blotted,and
R11820 T15334 T15335 arg1Of blotted,onto
R11821 T15337 T15335 arg2Of filters,onto
R11822 T15337 T15336 arg1Of filters,nitrocellulose
R11823 T15339 T15340 arg1Of Protran,;
R11824 T15340 T15338 arg2Of ;,(
R11825 T15344 T15340 arg2Of GmbH,;
R11826 T15344 T15341 arg1Of GmbH,Schleicher
R11827 T15344 T15342 arg1Of GmbH,&
R11828 T15344 T15343 arg1Of GmbH,Schuell
R11829 T15344 T15345 arg1Of GmbH,","
R11830 T15346 T15345 arg2Of Dassel,","
R11831 T15346 T15347 arg1Of Dassel,","
R11832 T15348 T15347 arg2Of Germany,","
R11833 T15349 T15338 arg3Of ),(
R11834 T15350 T15351 arg1Of Blocking,","
R11835 T15351 T15353 arg1Of ",",and
R11836 T15352 T15351 arg2Of washing,","
R11837 T15353 T15355 arg1Of and,of
R11838 T15353 T15361 arg1Of and,were
R11839 T15353 T15362 arg2Of and,done
R11840 T15354 T15353 arg2Of incubation,and
R11841 T15357 T15355 arg2Of filters,of
R11842 T15357 T15356 arg1Of filters,the
R11843 T15357 T15358 arg1Of filters,with
R11844 T15360 T15358 arg2Of antibodies,with
R11845 T15360 T15359 arg1Of antibodies,primary
R11846 T15362 T15361 arg2Of done,were
R11847 T15362 T15363 arg1Of done,according
R11848 T15362 T15369 arg1Of done,at
R11849 T15364 T15363 arg2Of to,according
R11850 T15366 T15365 arg1Of manufacturer,the
R11851 T15366 T15367 arg2Of manufacturer,'s
R11852 T15368 T15364 arg2Of protocols,to
R11853 T15368 T15367 arg1Of protocols,'s
R11854 T15371 T15369 arg2Of temperature,at
R11855 T15371 T15370 arg1Of temperature,room
R11856 T15371 T15372 arg1Of temperature,(
R11857 T15373 T15372 arg2Of RT,(
R11858 T15374 T15372 arg3Of ),(
R11859 T15376 T15375 arg2Of washing,After
R11860 T15376 T15377 arg1Of washing,with
R11861 T15379 T15377 arg2Of %,with
R11862 T15379 T15378 arg1Of %,TBS/0.1
R11863 T15379 T15380 arg1Of %,Tween-20
R11864 T15379 T15381 arg1Of %,(
R11865 T15382 T15381 arg2Of TBS-T,(
R11866 T15383 T15381 arg3Of ),(
R11867 T15386 T15385 arg1Of filters,the
R11868 T15386 T15387 arg1Of filters,were
R11869 T15386 T15388 arg2Of filters,incubated
R11870 T15388 T15376 arg2Of incubated,washing
R11871 T15388 T15384 arg1Of incubated,","
R11872 T15388 T15387 arg2Of incubated,were
R11873 T15388 T15389 arg1Of incubated,with
R11874 T15391 T15389 arg2Of peroxidase,with
R11875 T15391 T15390 arg1Of peroxidase,horseradish
R11876 T15391 T15392 arg1Of peroxidase,(
R11877 T15391 T15395 arg2Of peroxidase,coupled
R11878 T15393 T15392 arg2Of HRP,(
R11879 T15394 T15392 arg3Of ),(
R11880 T15395 T15396 arg1Of coupled,to
R11881 T15399 T15396 arg2Of antibodies,to
R11882 T15399 T15397 arg1Of antibodies,relevant
R11883 T15399 T15398 arg1Of antibodies,secondary
R11884 T15401 T15375 arg1Of see,After
R11885 T15401 T15400 arg1Of see,(
R11886 T15401 T15402 arg1Of see,above
R11887 T15401 T15403 arg1Of see,)
R11888 T15401 T15404 arg1Of see,for
R11889 T15406 T15404 arg2Of minutes,for
R11890 T15406 T15405 arg1Of minutes,60
R11891 T15406 T15407 arg1Of minutes,at
R11892 T15408 T15401 arg2Of RT,see
R11893 T15410 T15409 arg1Of activity,Enzyme
R11894 T15410 T15411 arg1Of activity,was
R11895 T15410 T15412 arg2Of activity,visualised
R11896 T15412 T15411 arg2Of visualised,was
R11897 T15412 T15420 arg1Of visualised,(
R11898 T15417 T15412 arg1Of system,visualised
R11899 T15417 T15413 arg2Of system,by
R11900 T15417 T15414 arg1Of system,the
R11901 T15417 T15415 arg2Of system,enhanced
R11902 T15417 T15416 arg1Of system,chemiluminescence
R11903 T15417 T15418 arg1Of system,","
R11904 T15419 T15418 arg2Of ECL+PLUS,","
R11905 T15421 T15422 arg1Of Amersham,","
R11906 T15422 T15420 arg2Of ",",(
R11907 T15422 T15424 arg1Of ",",","
R11908 T15423 T15422 arg2Of Buckinghamshire,","
R11909 T15425 T15424 arg2Of UK,","
R11910 T15426 T15420 arg3Of ),(
R11911 T15428 T15427 arg1Of analysis,Densitometric
R11912 T15428 T15429 arg1Of analysis,was
R11913 T15428 T15430 arg2Of analysis,performed
R11914 T15430 T15429 arg2Of performed,was
R11915 T15433 T15430 arg1Of hyperfilms,performed
R11916 T15433 T15431 arg2Of hyperfilms,by
R11917 T15433 T15432 arg1Of hyperfilms,scanning
R11918 T15433 T15434 arg1Of hyperfilms,on
R11919 T15438 T15434 arg2Of SI,on
R11920 T15438 T15435 arg1Of SI,a
R11921 T15438 T15436 arg1Of SI,Personal
R11922 T15438 T15437 arg1Of SI,Densitometer
R11923 T15438 T15439 arg1Of SI,(
R11924 T15441 T15439 arg2Of Dynamics,(
R11925 T15441 T15440 arg1Of Dynamics,Molecular
R11926 T15441 T15442 arg1Of Dynamics,","
R11927 T15443 T15442 arg2Of Sunnyvale,","
R11928 T15443 T15444 arg1Of Sunnyvale,","
R11929 T15445 T15444 arg2Of CA,","
R11930 T15446 T15439 arg3Of ),(
R11931 T15447 T15448 arg1Of Quantification,of
R11932 T15447 T15455 arg1Of Quantification,was
R11933 T15447 T15456 arg2Of Quantification,calculated
R11934 T15449 T15450 arg1Of Id1,","
R11935 T15450 T15452 arg1Of ",",and
R11936 T15451 T15450 arg2Of Id2,","
R11937 T15453 T15452 arg2Of Id3,and
R11938 T15454 T15448 arg2Of protein,of
R11939 T15454 T15449 arg1Of protein,Id1
R11940 T15454 T15451 arg1Of protein,Id2
R11941 T15454 T15453 arg1Of protein,Id3
R11942 T15456 T15455 arg2Of calculated,was
R11943 T15456 T15457 arg1Of calculated,by
R11944 T15458 T15457 arg2Of normalizing,by
R11945 T15462 T15458 arg2Of bands,normalizing
R11946 T15462 T15459 arg1Of bands,the
R11947 T15462 T15460 arg1Of bands,specific
R11948 T15462 T15461 arg1Of bands,protein
R11949 T15462 T15463 arg1Of bands,to
R11950 T15462 T15465 arg1Of bands,using
R11951 T15464 T15463 arg2Of β-actin,to
R11952 T15469 T15465 arg2Of software,using
R11953 T15469 T15466 arg1Of software,Image
R11954 T15469 T15467 arg1Of software,Quant
R11955 T15469 T15468 arg1Of software,5.5
R11956 T15469 T15470 arg1Of software,(
R11957 T15472 T15470 arg2Of Dynamics,(
R11958 T15472 T15471 arg1Of Dynamics,Molecular
R11959 T15473 T15470 arg3Of ),(
R11992 T15608 T15609 arg1Of Analysis,of
R11993 T15612 T15609 arg2Of RNA,of
R11994 T15612 T15610 arg1Of RNA,BMP-6
R11995 T15612 T15611 arg1Of RNA,messenger
R11996 T15612 T15613 arg1Of RNA,(
R11997 T15614 T15613 arg2Of mRNA,(
R11998 T15615 T15613 arg3Of ),(
R11999 T15616 T15608 arg1Of expression,Analysis
R12000 T15618 T15617 arg1Of expression,Endogenous
R12001 T15618 T15619 arg1Of expression,of
R12002 T15618 T15623 arg1Of expression,was
R12003 T15618 T15624 arg2Of expression,examined
R12004 T15622 T15619 arg2Of gene,of
R12005 T15622 T15620 arg1Of gene,the
R12006 T15622 T15621 arg1Of gene,BMP-6
R12007 T15624 T15623 arg2Of examined,was
R12008 T15629 T15624 arg1Of reaction,examined
R12009 T15629 T15625 arg2Of reaction,by
R12010 T15629 T15626 arg1Of reaction,reverse
R12011 T15629 T15627 arg1Of reaction,transcription-polymerase
R12012 T15629 T15628 arg1Of reaction,chain
R12013 T15631 T15630 arg1Of RNA,Total
R12014 T15631 T15632 arg1Of RNA,was
R12015 T15631 T15633 arg2Of RNA,isolated
R12016 T15633 T15632 arg2Of isolated,was
R12017 T15634 T15633 arg3Of using,isolated
R12018 T15634 T15635 arg1Of using,Absolutely
R12019 T15634 T15648 arg1Of using,according
R12020 T15638 T15636 arg1Of Miniprep,RNA™
R12021 T15638 T15637 arg1Of Miniprep,RT-PCR
R12022 T15639 T15634 arg2Of Kit,using
R12023 T15639 T15638 arg1Of Kit,Miniprep
R12024 T15639 T15640 arg1Of Kit,(
R12025 T15642 T15641 arg1Of Europe,Stratagene
R12026 T15642 T15643 arg1Of Europe,","
R12027 T15643 T15640 arg2Of ",",(
R12028 T15643 T15645 arg1Of ",",","
R12029 T15643 T15646 arg1Of ",",Netherland
R12030 T15644 T15643 arg2Of Amsterdam,","
R12031 T15647 T15640 arg3Of ),(
R12032 T15649 T15648 arg2Of to,according
R12033 T15652 T15649 arg2Of instructions,to
R12034 T15652 T15650 arg1Of instructions,the
R12035 T15652 T15651 arg1Of instructions,manufacturers
R12036 T15653 T15654 arg1Of Quantification,of
R12037 T15653 T15659 arg1Of Quantification,was
R12038 T15653 T15660 arg2Of Quantification,achieved
R12039 T15658 T15654 arg2Of RNA,of
R12040 T15658 T15655 arg1Of RNA,the
R12041 T15658 T15656 arg2Of RNA,isolated
R12042 T15658 T15657 arg1Of RNA,total
R12043 T15660 T15659 arg2Of achieved,was
R12044 T15660 T15661 arg1Of achieved,by
R12045 T15662 T15661 arg2Of using,by
R12046 T15665 T15662 arg2Of measurements,using
R12047 T15665 T15663 arg1Of measurements,spectrophotometric
R12048 T15665 T15664 arg1Of measurements,OD260
R12049 T15667 T15666 arg1Of amounts,Equal
R12050 T15667 T15668 arg1Of amounts,of
R12051 T15667 T15670 arg1Of amounts,were
R12052 T15669 T15668 arg2Of RNA,of
R12053 T15670 T15671 arg1Of were,then
R12054 T15672 T15670 arg2Of reverse,were
R12055 T15672 T15673 modOf reverse,transcribed
R12056 T15673 T15674 arg1Of transcribed,to
R12057 T15675 T15674 arg2Of cDNA,to
R12058 T15675 T15676 arg1Of cDNA,with
R12059 T15678 T15677 arg1Of Reverse,TaqMan®
R12060 T15680 T15676 arg2Of Reagents,with
R12061 T15680 T15678 arg1Of Reagents,Reverse
R12062 T15680 T15679 arg1Of Reagents,Transcription
R12063 T15680 T15681 arg1Of Reagents,(
R12064 T15683 T15682 arg1Of Biosystems,Applied
R12065 T15683 T15684 arg1Of Biosystems,","
R12066 T15684 T15687 arg1Of ",",","
R12067 T15686 T15684 arg2Of City,","
R12068 T15686 T15685 arg1Of City,Foster
R12069 T15687 T15681 arg2Of ",",(
R12070 T15687 T15689 arg1Of ",",","
R12071 T15688 T15687 arg2Of CA,","
R12072 T15690 T15689 arg2Of U.S.A,","
R12073 T15691 T15681 arg3Of ),(
R12074 T15693 T15692 arg1Of measure,To
R12075 T15695 T15693 arg2Of expression,measure
R12076 T15695 T15694 arg1Of expression,mRNA
R12077 T15695 T15696 arg1Of expression,of
R12078 T15697 T15696 arg2Of BMP6,of
R12079 T15699 T15700 arg1Of Id1–Id4,and
R12080 T15701 T15700 arg2Of PGK1,and
R12081 T15702 T15693 arg1Of PCR,measure
R12082 T15702 T15699 arg1Of PCR,Id1–Id4
R12083 T15702 T15701 arg1Of PCR,PGK1
R12084 T15702 T15703 arg1Of PCR,were
R12085 T15702 T15704 arg2Of PCR,carried
R12086 T15704 T15692 modOf carried,To
R12087 T15704 T15698 arg1Of carried,","
R12088 T15704 T15703 arg2Of carried,were
R12089 T15704 T15705 arg1Of carried,out
R12090 T15704 T15706 arg1Of carried,with
R12091 T15710 T15706 arg2Of mix,with
R12092 T15710 T15707 arg1Of mix,TaqMan®
R12093 T15710 T15708 arg1Of mix,universal
R12094 T15710 T15709 arg1Of mix,master
R12095 T15711 T15712 arg1Of Primers,and
R12096 T15712 T15714 arg1Of and,were
R12097 T15712 T15715 arg2Of and,provided
R12098 T15713 T15712 arg2Of probes,and
R12099 T15715 T15714 arg2Of provided,were
R12100 T15717 T15715 arg1Of Assay-on-Demand,provided
R12101 T15717 T15716 arg2Of Assay-on-Demand,by
R12102 T15717 T15718 arg1Of Assay-on-Demand,(
R12103 T15720 T15718 arg2Of Biosystems,(
R12104 T15720 T15719 arg1Of Biosystems,Applied
R12105 T15721 T15718 arg3Of ),(
R12106 T15723 T15722 arg1Of reactions,PCR
R12107 T15723 T15724 arg1Of reactions,were
R12108 T15723 T15725 arg2Of reactions,carried
R12109 T15725 T15724 arg2Of carried,were
R12110 T15725 T15726 arg1Of carried,out
R12111 T15725 T15727 arg1Of carried,in
R12112 T15730 T15727 arg2Of volume,in
R12113 T15730 T15728 arg1Of volume,a
R12114 T15730 T15729 arg1Of volume,final
R12115 T15730 T15731 arg1Of volume,of
R12116 T15733 T15732 arg1Of μl,25
R12117 T15733 T15734 arg1Of μl,(
R12118 T15733 T15737 arg1Of μl,or
R12119 T15735 T15734 arg2Of BMP-6,(
R12120 T15736 T15734 arg3Of ),(
R12121 T15737 T15731 arg2Of or,of
R12122 T15739 T15737 arg2Of μl,or
R12123 T15739 T15738 arg1Of μl,20
R12124 T15739 T15740 arg1Of μl,(
R12125 T15741 T15740 arg2Of ID1,(
R12126 T15742 T15740 arg3Of ),(
R12127 T15744 T15743 arg1Of cDNA,The
R12128 T15744 T15745 arg2Of cDNA,added
R12129 T15744 T15749 arg1Of cDNA,was
R12130 T15744 T15750 arg1Of cDNA,equivalent
R12131 T15745 T15746 arg1Of added,to
R12132 T15748 T15746 arg2Of reaction,to
R12133 T15748 T15747 arg1Of reaction,each
R12134 T15750 T15749 arg2Of equivalent,was
R12135 T15750 T15751 arg1Of equivalent,to
R12136 T15753 T15751 arg2Of input,to
R12137 T15753 T15752 arg1Of input,the
R12138 T15753 T15754 arg1Of input,of
R12139 T15756 T15754 arg2Of ng,of
R12140 T15756 T15755 arg1Of ng,20
R12141 T15756 T15757 arg1Of ng,of
R12142 T15759 T15757 arg2Of RNA,of
R12143 T15759 T15758 arg1Of RNA,total
R12144 T15762 T15760 arg1Of expression,The
R12145 T15762 T15761 arg1Of expression,gene
R12146 T15762 T15763 arg1Of expression,was
R12147 T15762 T15764 arg2Of expression,quantified
R12148 T15762 T15765 arg1Of expression,using
R12149 T15764 T15763 arg2Of quantified,was
R12150 T15765 T15764 arg3Of using,quantified
R12151 T15765 T15782 arg1Of using,as
R12152 T15769 T15766 arg1Of method,the
R12153 T15769 T15767 arg1Of method,standard
R12154 T15769 T15768 arg1Of method,curve
R12155 T15769 T15770 arg1Of method,(
R12156 T15769 T15774 arg1Of method,or
R12157 T15771 T15770 arg2Of BMP6,(
R12158 T15772 T15770 arg3Of ),(
R12159 T15774 T15765 arg2Of or,using
R12160 T15774 T15773 arg1Of or,","
R12161 T15778 T15774 arg2Of method,or
R12162 T15778 T15775 arg1Of method,the
R12163 T15778 T15776 arg1Of method,comparative
R12164 T15778 T15777 arg1Of method,CT
R12165 T15778 T15779 arg1Of method,(
R12166 T15780 T15779 arg2Of Id1,(
R12167 T15781 T15779 arg3Of ),(
R12168 T15783 T15782 arg2Of described,as
R12169 T15783 T15784 arg1Of described,in
R12170 T15787 T15784 arg2Of Bulletin,in
R12171 T15787 T15785 arg1Of Bulletin,ABI7700
R12172 T15787 T15786 arg1Of Bulletin,User
R12173 T15787 T15788 arg1Of Bulletin,2
R12174 T15787 T15789 arg1Of Bulletin,(
R12175 T15791 T15789 arg2Of Biosystems,(
R12176 T15791 T15790 arg1Of Biosystems,Applied
R12177 T15792 T15789 arg3Of ),(
R12178 T15794 T15793 arg1Of expression,The
R12179 T15794 T15795 arg1Of expression,was
R12180 T15794 T15797 arg2Of expression,normalized
R12181 T15797 T15795 arg2Of normalized,was
R12182 T15797 T15796 arg1Of normalized,then
R12183 T15797 T15798 arg1Of normalized,to
R12184 T15801 T15798 arg2Of level,to
R12185 T15801 T15799 arg1Of level,the
R12186 T15801 T15800 arg1Of level,expression
R12187 T15801 T15802 arg1Of level,of
R12188 T15803 T15802 arg2Of PGK1,of
R12189 T15804 T15805 arg1Of PGK1,was
R12190 T15804 T15806 arg2Of PGK1,chosen
R12191 T15806 T15805 arg2Of chosen,was
R12192 T15806 T15807 arg1Of chosen,","
R12193 T15806 T15808 arg1Of chosen,because
R12194 T15809 T15810 arg1Of it,has
R12195 T15809 T15811 arg1Of it,been
R12196 T15809 T15812 arg2Of it,shown
R12197 T15809 T15814 arg1Of it,have
R12198 T15812 T15808 arg2Of shown,because
R12199 T15812 T15810 arg2Of shown,has
R12200 T15812 T15811 arg2Of shown,been
R12201 T15812 T15821 arg1Of shown,[
R12202 T15814 T15812 arg3Of have,shown
R12203 T15814 T15813 arg1Of have,to
R12204 T15817 T15814 arg2Of variability,have
R12205 T15817 T15815 arg1Of variability,low
R12206 T15817 T15816 arg1Of variability,expression
R12207 T15817 T15818 arg1Of variability,among
R12208 T15820 T15818 arg2Of specimens,among
R12209 T15820 T15819 arg1Of specimens,lymphocyte
R12210 T15822 T15821 arg2Of 56,[
R12211 T15823 T15821 arg3Of ],[
R12212 T15825 T15824 arg1Of levels,Expression
R12213 T15825 T15826 arg1Of levels,in
R12214 T15825 T15829 arg1Of levels,were
R12215 T15825 T15831 arg2Of levels,related
R12216 T15828 T15826 arg2Of cells,in
R12217 T15828 T15827 arg1Of cells,B
R12218 T15831 T15829 arg2Of related,were
R12219 T15831 T15830 arg1Of related,then
R12220 T15831 T15832 arg1Of related,to
R12221 T15835 T15832 arg2Of levels,to
R12222 T15835 T15833 arg1Of levels,the
R12223 T15835 T15834 arg1Of levels,expression
R12224 T15835 T15836 arg1Of levels,in
R12225 T15838 T15836 arg2Of cells,in
R12226 T15838 T15837 arg1Of cells,Ramos
R12526 T16238 T16237 arg1Of proliferation,Cell
R12527 T16240 T16241 arg1Of estimation,of
R12528 T16240 T16239 arg2Of estimation,For
R12529 T16243 T16241 arg2Of synthesis,of
R12530 T16243 T16242 arg1Of synthesis,DNA
R12531 T16246 T16245 arg1Of cells,CD19+
R12532 T16246 T16247 arg1Of cells,(
R12533 T16246 T16254 arg1Of cells,or
R12534 T16248 T16249 arg1Of 7.5,×
R12535 T16250 T16249 arg2Of 104,×
R12536 T16252 T16247 arg2Of ml,(
R12537 T16252 T16248 arg1Of ml,7.5
R12538 T16252 T16250 arg1Of ml,104
R12539 T16252 T16251 arg1Of ml,cells/0.2
R12540 T16253 T16247 arg3Of ),(
R12541 T16254 T16264 arg1Of or,were
R12542 T16254 T16265 arg2Of or,cultured
R12543 T16256 T16254 arg2Of cells,or
R12544 T16256 T16255 arg1Of cells,Ramos
R12545 T16256 T16257 arg1Of cells,(
R12546 T16262 T16257 arg2Of ml,(
R12547 T16262 T16258 arg1Of ml,1
R12548 T16262 T16259 arg1Of ml,×
R12549 T16262 T16260 arg1Of ml,104
R12550 T16262 T16261 arg1Of ml,cells/0.2
R12551 T16263 T16257 arg3Of ),(
R12552 T16265 T16244 arg1Of cultured,","
R12553 T16265 T16239 arg1Of cultured,For
R12554 T16265 T16264 arg2Of cultured,were
R12555 T16265 T16266 arg1Of cultured,in
R12556 T16267 T16266 arg2Of triplicate,in
R12557 T16267 T16268 arg1Of triplicate,in
R12558 T16270 T16268 arg2Of wells,in
R12559 T16270 T16269 arg1Of wells,microtiter
R12560 T16272 T16271 arg1Of cells,The
R12561 T16272 T16273 arg1Of cells,were
R12562 T16272 T16274 arg2Of cells,pulsed
R12563 T16274 T16273 arg2Of pulsed,were
R12564 T16274 T16275 arg1Of pulsed,with
R12565 T16276 T16277 arg1Of 3.7,×
R12566 T16277 T16275 arg2Of ×,with
R12567 T16280 T16279 arg2Of 3H,[
R12568 T16281 T16279 arg3Of ],[
R12569 T16282 T16277 arg2Of thymidine,×
R12570 T16282 T16278 arg1Of thymidine,104Bq
R12571 T16282 T16279 arg1Of thymidine,[
R12572 T16282 T16283 arg1Of thymidine,(
R12573 T16282 T16290 arg1Of thymidine,for
R12574 T16284 T16285 arg1Of Amersham,","
R12575 T16285 T16283 arg2Of ",",(
R12576 T16285 T16287 arg1Of ",",","
R12577 T16286 T16285 arg2Of Buckinghamshire,","
R12578 T16288 T16287 arg2Of UK,","
R12579 T16289 T16283 arg3Of ),(
R12580 T16294 T16290 arg2Of h,for
R12581 T16294 T16291 arg1Of h,the
R12582 T16294 T16292 arg1Of h,last
R12583 T16294 T16293 arg1Of h,16
R12584 T16294 T16295 arg1Of h,of
R12585 T16298 T16295 arg2Of incubation,of
R12586 T16298 T16296 arg1Of incubation,a
R12587 T16298 T16297 arg1Of incubation,72-h
R12588 T16300 T16299 arg1Of cells,The
R12589 T16300 T16301 arg1Of cells,were
R12590 T16300 T16302 arg2Of cells,harvested
R12591 T16302 T16301 arg2Of harvested,were
R12592 T16302 T16319 arg1Of harvested,and
R12593 T16303 T16302 arg3Of using,harvested
R12594 T16303 T16308 arg1Of using,(
R12595 T16307 T16303 arg2Of harvester,using
R12596 T16307 T16304 arg1Of harvester,an
R12597 T16307 T16305 arg2Of harvester,automated
R12598 T16307 T16306 arg1Of harvester,cell
R12599 T16311 T16308 arg2Of Company,(
R12600 T16311 T16309 arg1Of Company,Packard
R12601 T16311 T16310 arg1Of Company,Instrument
R12602 T16311 T16312 arg1Of Company,","
R12603 T16311 T16316 arg1Of Company,","
R12604 T16311 T16317 arg1Of Company,USA
R12605 T16313 T16314 arg1Of Meriden,","
R12606 T16314 T16312 arg2Of ",",","
R12607 T16315 T16314 arg2Of CT,","
R12608 T16318 T16308 arg3Of ),(
R12609 T16321 T16320 arg2Of 3H,[
R12610 T16322 T16320 arg3Of ],[
R12611 T16324 T16320 arg1Of incorporation,[
R12612 T16324 T16323 arg1Of incorporation,thymidine
R12613 T16324 T16325 arg1Of incorporation,was
R12614 T16324 T16326 arg2Of incorporation,determined
R12615 T16326 T16319 arg2Of determined,and
R12616 T16326 T16325 arg2Of determined,was
R12617 T16326 T16327 arg1Of determined,in
R12618 T16326 T16331 arg1Of determined,(
R12619 T16330 T16327 arg2Of counter,in
R12620 T16330 T16328 arg1Of counter,a
R12621 T16330 T16329 arg1Of counter,scintillation
R12622 T16332 T16333 arg1Of TopCount,","
R12623 T16333 T16338 arg1Of ",",","
R12624 T16337 T16333 arg2Of Inc.,","
R12625 T16337 T16334 arg1Of Inc.,Packard
R12626 T16337 T16335 arg1Of Inc.,Instrument
R12627 T16337 T16336 arg1Of Inc.,Company
R12628 T16338 T16331 arg2Of ",",(
R12629 T16338 T16340 arg1Of ",",","
R12630 T16339 T16338 arg2Of Meriden,","
R12631 T16341 T16340 arg2Of CT,","
R12632 T16342 T16331 arg3Of ),(
R12745 T16481 T16482 arg1Of Determination,of
R12746 T16484 T16482 arg2Of death,of
R12747 T16484 T16483 arg1Of death,cell
R12748 T16486 T16485 arg1Of death,Cell
R12749 T16486 T16487 arg1Of death,was
R12750 T16486 T16488 arg2Of death,measured
R12751 T16486 T16495 arg1Of death,staining
R12752 T16488 T16487 arg2Of measured,was
R12753 T16488 T16494 arg1Of measured,by
R12754 T16488 T16518 arg1Of measured,for
R12755 T16493 T16488 arg1Of test,measured
R12756 T16493 T16489 arg2Of test,by
R12757 T16493 T16490 arg1Of test,vital
R12758 T16493 T16491 arg1Of test,dye
R12759 T16493 T16492 arg1Of test,exclusion
R12760 T16495 T16494 arg2Of staining,by
R12761 T16495 T16497 arg1Of staining,with
R12762 T16496 T16495 arg2Of cells,staining
R12763 T16501 T16497 arg2Of iodide,with
R12764 T16501 T16498 arg1Of iodide,5
R12765 T16501 T16499 arg1Of iodide,μg/ml
R12766 T16501 T16500 arg1Of iodide,propidium
R12767 T16501 T16502 arg1Of iodide,(
R12768 T16504 T16503 arg2Of PI,[
R12769 T16504 T16506 arg1Of PI,;
R12770 T16505 T16503 arg3Of ],[
R12771 T16506 T16509 arg1Of ;,;
R12772 T16508 T16506 arg2Of Corp.,;
R12773 T16508 T16507 arg1Of Corp.,Calbiochem
R12774 T16509 T16514 arg1Of ;,;
R12775 T16511 T16509 arg2Of Jolla,;
R12776 T16511 T16510 arg1Of Jolla,La
R12777 T16511 T16512 arg1Of Jolla,","
R12778 T16513 T16512 arg2Of CA,","
R12779 T16514 T16502 arg2Of ;,(
R12780 T16516 T16514 arg2Of mg/ml,;
R12781 T16516 T16515 arg1Of mg/ml,5
R12782 T16517 T16502 arg3Of ),(
R12783 T16520 T16518 arg2Of minute,for
R12784 T16520 T16519 arg1Of minute,one
R12785 T16520 T16521 arg1Of minute,on
R12786 T16522 T16521 arg2Of ice,on
R12787 T16525 T16523 arg1Of "1,000",At
R12788 T16525 T16524 arg1Of "1,000",least
R12789 T16526 T16525 arg1Of cells,"1,000"
R12790 T16526 T16527 arg1Of cells,per
R12791 T16526 T16529 arg1Of cells,were
R12792 T16526 T16530 arg2Of cells,run
R12793 T16528 T16527 arg2Of sample,per
R12794 T16530 T16529 arg2Of run,were
R12795 T16530 T16531 arg1Of run,on
R12796 T16536 T16531 arg2Of cytometer,on
R12797 T16536 T16532 arg1Of cytometer,a
R12798 T16536 T16533 arg1Of cytometer,BD
R12799 T16536 T16534 arg1Of cytometer,FACSCalibur
R12800 T16536 T16535 arg1Of cytometer,flow
R12861 T16610 T16609 arg1Of analysis,Statistical
R12862 T16613 T16611 arg1Of significance,The
R12863 T16613 T16612 arg1Of significance,statistical
R12864 T16613 T16614 arg1Of significance,of
R12865 T16613 T16616 arg1Of significance,between
R12866 T16613 T16618 arg1Of significance,was
R12867 T16613 T16619 arg2Of significance,determined
R12868 T16613 T16629 arg1Of significance,applying
R12869 T16615 T16614 arg2Of differences,of
R12870 T16617 T16616 arg2Of groups,between
R12871 T16619 T16618 arg2Of determined,was
R12872 T16619 T16627 arg1Of determined,","
R12873 T16619 T16628 arg1Of determined,by
R12874 T16620 T16619 arg3Of using,determined
R12875 T16626 T16620 arg2Of test,using
R12876 T16626 T16621 arg1Of test,the
R12877 T16626 T16622 arg1Of test,paired
R12878 T16626 T16623 arg1Of test,two-tailed
R12879 T16626 T16624 arg1Of test,Wilcoxon
R12880 T16626 T16625 arg1Of test,nonparametric
R12881 T16629 T16628 arg2Of applying,by
R12882 T16631 T16629 arg2Of software,applying
R12883 T16631 T16630 arg1Of software,SPSS10.1
R12884 T16631 T16632 arg1Of software,(
R12885 T16634 T16632 arg2Of Inc.,(
R12886 T16634 T16633 arg1Of Inc.,SPSS
R12887 T16634 T16635 arg1Of Inc.,","
R12888 T16634 T16639 arg1Of Inc.,","
R12889 T16634 T16640 arg1Of Inc.,USA
R12890 T16636 T16637 arg1Of Chicago,","
R12891 T16637 T16635 arg2Of ",",","
R12892 T16638 T16637 arg2Of IL,","
R12893 T16641 T16632 arg3Of ),(
R12894 T16643 T16642 arg1Of values,P
R12895 T16643 T16644 arg1Of values,less
R12896 T16643 T16647 arg1Of values,were
R12897 T16643 T16648 arg2Of values,considered
R12898 T16643 T16649 arg1Of values,significant
R12899 T16644 T16645 arg1Of less,than
R12900 T16646 T16645 arg2Of 0.05,than
R12901 T16648 T16647 arg2Of considered,were
R12902 T16649 T16648 arg3Of significant,considered