pmc-enju-pas Home  

PMC:1064895 / 12555-13752 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T9391 0-4 NN denotes PBMC
T9392 5-9 VB denotes were
T9393 10-19 VB denotes incubated
T9394 20-24 IN denotes with
T9395 25-33 NN denotes anti-CD3
T9396 34-35 -LRB- denotes (
T9397 35-37 CD denotes 10
T9398 38-43 NN denotes μg/ml
T9399 43-44 -RRB- denotes )
T9400 45-47 IN denotes in
T9401 48-51 DT denotes the
T9402 52-60 NN denotes presence
T9403 61-63 CC denotes or
T9404 64-71 NN denotes absence
T9405 72-74 IN denotes of
T9406 75-83 NN denotes LY294002
T9407 84-85 -LRB- denotes (
T9408 85-87 CD denotes 20
T9409 88-90 NN denotes μM
T9410 90-91 -RRB- denotes )
T9411 93-98 IN denotes After
T9412 99-109 NN denotes incubation
T9413 110-113 IN denotes for
T9414 114-115 CD denotes 1
T9415 116-120 NN denotes hour
T9416 120-121 -COMMA- denotes ,
T9417 122-127 JJ denotes whole
T9418 128-132 NN denotes cell
T9419 133-140 NN denotes lysates
T9420 141-145 VB denotes were
T9421 146-154 VB denotes prepared
T9422 155-159 IN denotes from
T9423 160-165 IN denotes about
T9424 166-169 CD denotes 107
T9425 170-175 NN denotes cells
T9426 176-178 IN denotes by
T9427 179-193 NN denotes homogenization
T9428 194-196 IN denotes in
T9429 197-200 DT denotes the
T9430 201-206 NN denotes lysis
T9431 207-213 NN denotes buffer
T9432 213-214 -COMMA- denotes ,
T9433 215-218 CC denotes and
T9434 219-230 VB denotes centrifuged
T9435 231-233 IN denotes at
T9436 234-240 CD denotes 14,000
T9437 241-247 NN denotes r.p.m.
T9438 248-249 -LRB- denotes (
T9439 249-255 CD denotes 19,000
T9440 256-257 NN denotes g
T9441 257-258 -RRB- denotes )
T9442 259-262 IN denotes for
T9443 263-265 CD denotes 15
T9444 266-269 NN denotes min
T9445 271-278 NN denotes Protein
T9446 279-293 NN denotes concentrations
T9447 294-296 IN denotes in
T9448 297-300 DT denotes the
T9449 301-313 NN denotes supernatants
T9450 314-318 VB denotes were
T9451 319-329 VB denotes determined
T9452 330-334 IN denotes with
T9453 335-338 DT denotes the
T9454 339-347 NNP denotes Bradford
T9455 348-354 NN denotes method
T9456 355-356 -LRB- denotes (
T9457 356-363 NNP denotes Bio-Rad
T9458 363-364 -COMMA- denotes ,
T9459 365-373 NNP denotes Hercules
T9460 373-374 -COMMA- denotes ,
T9461 375-377 NNP denotes CA
T9462 377-378 -COMMA- denotes ,
T9463 379-382 NNP denotes USA
T9464 382-383 -RRB- denotes )
T9465 385-392 NN denotes Protein
T9466 393-400 NN denotes samples
T9467 401-405 VB denotes were
T9468 406-415 VB denotes separated
T9469 416-418 IN denotes by
T9470 419-421 CD denotes 10
T9471 421-422 NN denotes %
T9472 423-431 NN denotes SDS–PAGE
T9473 432-435 CC denotes and
T9474 436-447 VB denotes transferred
T9475 448-450 TO denotes to
T9476 451-452 DT denotes a
T9477 453-467 NN denotes nitrocellulose
T9478 468-476 NN denotes membrane
T9479 477-478 -LRB- denotes (
T9480 478-486 NNP denotes Amersham
T9481 487-496 NNP denotes Pharmacia
T9482 497-504 NNP denotes Biotech
T9483 504-505 -COMMA- denotes ,
T9484 506-513 NNP denotes Uppsala
T9485 513-514 -COMMA- denotes ,
T9486 515-521 NNP denotes Sweden
T9487 521-522 -RRB- denotes )
T9488 524-527 IN denotes For
T9489 528-535 JJ denotes western
T9490 536-549 NN denotes hybridization
T9491 549-550 -COMMA- denotes ,
T9492 551-559 NN denotes membrane
T9493 560-563 VB denotes was
T9494 564-576 VB denotes preincubated
T9495 577-581 IN denotes with
T9496 582-585 CD denotes 0.1
T9497 585-586 NN denotes %
T9498 587-594 VB denotes skimmed
T9499 595-599 NN denotes milk
T9500 600-602 IN denotes in
T9501 603-608 NN denotes TBS-T
T9502 609-615 NN denotes buffer
T9503 616-617 -LRB- denotes (
T9504 617-620 CD denotes 0.1
T9505 620-621 NN denotes %
T9506 622-627 CD denotes Tween
T9507 628-630 CD denotes 20
T9508 631-633 IN denotes in
T9509 634-647 JJ denotes Tris-buffered
T9510 648-654 NN denotes saline
T9511 654-655 -RRB- denotes )
T9512 656-658 IN denotes at
T9513 659-663 NN denotes room
T9514 664-675 NN denotes temperature
T9515 676-679 IN denotes for
T9516 680-681 CD denotes 2
T9517 682-687 NN denotes hours
T9518 687-688 -COMMA- denotes ,
T9519 689-693 RB denotes then
T9520 694-701 JJ denotes primary
T9521 702-712 NN denotes antibodies
T9522 713-720 IN denotes against
T9523 721-724 NNP denotes Akt
T9524 724-725 -COMMA- denotes ,
T9525 726-740 VB denotes phosphorylated
T9526 741-744 NN denotes Akt
T9527 745-748 CC denotes and
T9528 749-754 NN denotes IκB-α
T9529 755-756 -LRB- denotes (
T9530 756-760 NNP denotes Cell
T9531 761-770 NNP denotes Signaling
T9532 771-781 NNP denotes Technology
T9533 782-786 NNP denotes Inc.
T9534 786-787 -COMMA- denotes ,
T9535 788-795 NNP denotes Beverly
T9536 795-796 -COMMA- denotes ,
T9537 797-799 NNP denotes MA
T9538 799-800 -COMMA- denotes ,
T9539 801-804 NNP denotes USA
T9540 804-805 -RRB- denotes )
T9541 805-806 -COMMA- denotes ,
T9542 807-814 VB denotes diluted
T9543 815-816 CD denotes 1
T9544 816-817 -COLON- denotes :
T9545 817-821 CD denotes 1000
T9546 822-824 IN denotes in
T9547 825-826 CD denotes 5
T9548 826-827 NN denotes %
T9549 828-837 NN denotes BSA/TBS-T
T9550 837-838 -COMMA- denotes ,
T9551 839-843 VB denotes were
T9552 844-849 VB denotes added
T9553 850-853 CC denotes and
T9554 854-863 VB denotes incubated
T9555 864-873 RB denotes overnight
T9556 874-876 IN denotes at
T9557 877-880 NN denotes 4°C
T9558 882-887 IN denotes After
T9559 888-895 VB denotes washing
T9560 896-900 CD denotes four
T9561 901-906 NN denotes times
T9562 907-911 IN denotes with
T9563 912-917 NN denotes TBS-T
T9564 917-918 -COMMA- denotes ,
T9565 919-930 JJ denotes horseradish
T9566 931-952 JJ denotes peroxidase-conjugated
T9567 953-962 JJ denotes secondary
T9568 963-973 NN denotes antibodies
T9569 974-978 VB denotes were
T9570 979-984 VB denotes added
T9571 985-988 CC denotes and
T9572 989-996 VB denotes allowed
T9573 997-999 TO denotes to
T9574 1000-1008 VB denotes incubate
T9575 1009-1012 IN denotes for
T9576 1013-1014 CD denotes 1
T9577 1015-1019 NN denotes hour
T9578 1020-1022 IN denotes at
T9579 1023-1027 NN denotes room
T9580 1028-1039 NN denotes temperature
T9581 1041-1046 IN denotes After
T9582 1047-1052 NN denotes TBS-T
T9583 1053-1060 NN denotes washing
T9584 1060-1061 -COMMA- denotes ,
T9585 1062-1072 VB denotes hybridized
T9586 1073-1078 NN denotes bands
T9587 1079-1083 VB denotes were
T9588 1084-1092 VB denotes detected
T9589 1093-1097 IN denotes with
T9590 1098-1101 DT denotes the
T9591 1102-1110 VB denotes enhanced
T9592 1111-1128 NN denotes chemiluminescence
T9593 1129-1130 -LRB- denotes (
T9594 1130-1133 NN denotes ECL
T9595 1133-1134 -RRB- denotes )
T9596 1135-1144 NN denotes detection
T9597 1145-1148 NN denotes kit
T9598 1149-1152 CC denotes and
T9599 1153-1166 NN denotes Hyperfilm-ECL
T9600 1167-1175 NN denotes reagents
T9601 1176-1177 -LRB- denotes (
T9602 1177-1185 NNP denotes Amersham
T9603 1186-1195 NNP denotes Pharmacia
T9604 1195-1196 -RRB- denotes )
R4843 T9391 T9392 arg1Of PBMC,were
R4844 T9391 T9393 arg2Of PBMC,incubated
R4845 T9393 T9392 arg2Of incubated,were
R4846 T9393 T9394 arg1Of incubated,with
R4847 T9393 T9400 arg1Of incubated,in
R4848 T9395 T9394 arg2Of anti-CD3,with
R4849 T9395 T9396 arg1Of anti-CD3,(
R4850 T9398 T9396 arg2Of μg/ml,(
R4851 T9398 T9397 arg1Of μg/ml,10
R4852 T9399 T9396 arg3Of ),(
R4853 T9402 T9403 arg1Of presence,or
R4854 T9403 T9400 arg2Of or,in
R4855 T9403 T9401 arg1Of or,the
R4856 T9403 T9405 arg1Of or,of
R4857 T9404 T9403 arg2Of absence,or
R4858 T9406 T9405 arg2Of LY294002,of
R4859 T9406 T9407 arg1Of LY294002,(
R4860 T9409 T9407 arg2Of μM,(
R4861 T9409 T9408 arg1Of μM,20
R4862 T9410 T9407 arg3Of ),(
R4863 T9412 T9411 arg2Of incubation,After
R4864 T9412 T9413 arg1Of incubation,for
R4865 T9415 T9413 arg2Of hour,for
R4866 T9415 T9414 arg1Of hour,1
R4867 T9419 T9417 arg1Of lysates,whole
R4868 T9419 T9418 arg1Of lysates,cell
R4869 T9419 T9420 arg1Of lysates,were
R4870 T9419 T9421 arg2Of lysates,prepared
R4871 T9419 T9434 arg2Of lysates,centrifuged
R4872 T9421 T9422 arg1Of prepared,from
R4873 T9421 T9433 arg1Of prepared,and
R4874 T9424 T9423 arg1Of 107,about
R4875 T9425 T9422 arg2Of cells,from
R4876 T9425 T9424 arg1Of cells,107
R4877 T9427 T9421 arg1Of homogenization,prepared
R4878 T9427 T9426 arg2Of homogenization,by
R4879 T9427 T9428 arg1Of homogenization,in
R4880 T9431 T9428 arg2Of buffer,in
R4881 T9431 T9429 arg1Of buffer,the
R4882 T9431 T9430 arg1Of buffer,lysis
R4883 T9433 T9411 arg1Of and,After
R4884 T9433 T9416 arg1Of and,","
R4885 T9433 T9420 arg2Of and,were
R4886 T9433 T9432 arg1Of and,","
R4887 T9434 T9433 arg2Of centrifuged,and
R4888 T9434 T9435 arg1Of centrifuged,at
R4889 T9434 T9442 arg1Of centrifuged,for
R4890 T9437 T9435 arg2Of r.p.m.,at
R4891 T9437 T9436 arg1Of r.p.m.,"14,000"
R4892 T9437 T9438 arg1Of r.p.m.,(
R4893 T9440 T9438 arg2Of g,(
R4894 T9440 T9439 arg1Of g,"19,000"
R4895 T9441 T9438 arg3Of ),(
R4896 T9444 T9442 arg2Of min,for
R4897 T9444 T9443 arg1Of min,15
R4898 T9446 T9445 arg1Of concentrations,Protein
R4899 T9446 T9447 arg1Of concentrations,in
R4900 T9446 T9450 arg1Of concentrations,were
R4901 T9446 T9451 arg2Of concentrations,determined
R4902 T9449 T9447 arg2Of supernatants,in
R4903 T9449 T9448 arg1Of supernatants,the
R4904 T9451 T9450 arg2Of determined,were
R4905 T9451 T9452 arg1Of determined,with
R4906 T9451 T9456 arg1Of determined,(
R4907 T9455 T9452 arg2Of method,with
R4908 T9455 T9453 arg1Of method,the
R4909 T9455 T9454 arg1Of method,Bradford
R4910 T9457 T9458 arg1Of Bio-Rad,","
R4911 T9458 T9460 arg1Of ",",","
R4912 T9459 T9458 arg2Of Hercules,","
R4913 T9460 T9456 arg2Of ",",(
R4914 T9460 T9462 arg1Of ",",","
R4915 T9460 T9463 arg1Of ",",USA
R4916 T9461 T9460 arg2Of CA,","
R4917 T9464 T9456 arg3Of ),(
R4918 T9466 T9465 arg1Of samples,Protein
R4919 T9466 T9467 arg1Of samples,were
R4920 T9466 T9468 arg2Of samples,separated
R4921 T9466 T9474 arg2Of samples,transferred
R4922 T9468 T9473 arg1Of separated,and
R4923 T9470 T9471 arg1Of 10,%
R4924 T9472 T9468 arg1Of SDS–PAGE,separated
R4925 T9472 T9469 arg2Of SDS–PAGE,by
R4926 T9472 T9470 arg1Of SDS–PAGE,10
R4927 T9473 T9467 arg2Of and,were
R4928 T9473 T9479 arg1Of and,(
R4929 T9474 T9473 arg2Of transferred,and
R4930 T9474 T9475 arg1Of transferred,to
R4931 T9478 T9475 arg2Of membrane,to
R4932 T9478 T9476 arg1Of membrane,a
R4933 T9478 T9477 arg1Of membrane,nitrocellulose
R4934 T9482 T9479 arg2Of Biotech,(
R4935 T9482 T9480 arg1Of Biotech,Amersham
R4936 T9482 T9481 arg1Of Biotech,Pharmacia
R4937 T9482 T9483 arg1Of Biotech,","
R4938 T9484 T9483 arg2Of Uppsala,","
R4939 T9484 T9485 arg1Of Uppsala,","
R4940 T9486 T9485 arg2Of Sweden,","
R4941 T9487 T9479 arg3Of ),(
R4942 T9490 T9488 arg2Of hybridization,For
R4943 T9490 T9489 arg1Of hybridization,western
R4944 T9492 T9493 arg1Of membrane,was
R4945 T9492 T9494 arg2Of membrane,preincubated
R4946 T9494 T9493 arg2Of preincubated,was
R4947 T9494 T9495 arg1Of preincubated,with
R4948 T9494 T9515 arg1Of preincubated,for
R4949 T9494 T9524 arg1Of preincubated,","
R4950 T9497 T9496 arg1Of %,0.1
R4951 T9499 T9495 arg2Of milk,with
R4952 T9499 T9497 arg1Of milk,%
R4953 T9499 T9498 arg2Of milk,skimmed
R4954 T9499 T9500 arg1Of milk,in
R4955 T9502 T9500 arg2Of buffer,in
R4956 T9502 T9501 arg1Of buffer,TBS-T
R4957 T9502 T9503 arg1Of buffer,(
R4958 T9502 T9512 arg1Of buffer,at
R4959 T9505 T9504 arg1Of %,0.1
R4960 T9507 T9503 arg2Of 20,(
R4961 T9507 T9505 arg1Of 20,%
R4962 T9507 T9506 arg1Of 20,Tween
R4963 T9507 T9508 arg1Of 20,in
R4964 T9510 T9508 arg2Of saline,in
R4965 T9510 T9509 arg1Of saline,Tris-buffered
R4966 T9511 T9503 arg3Of ),(
R4967 T9514 T9512 arg2Of temperature,at
R4968 T9514 T9513 arg1Of temperature,room
R4969 T9517 T9515 arg2Of hours,for
R4970 T9517 T9516 arg1Of hours,2
R4971 T9517 T9518 arg1Of hours,","
R4972 T9521 T9518 arg2Of antibodies,","
R4973 T9521 T9519 arg1Of antibodies,then
R4974 T9521 T9520 arg1Of antibodies,primary
R4975 T9521 T9522 arg1Of antibodies,against
R4976 T9523 T9522 arg2Of Akt,against
R4977 T9524 T9488 arg1Of ",",For
R4978 T9524 T9491 arg1Of ",",","
R4979 T9524 T9544 arg1Of ",",:
R4980 T9526 T9527 arg1Of Akt,and
R4981 T9527 T9525 arg2Of and,phosphorylated
R4982 T9527 T9529 arg1Of and,(
R4983 T9527 T9542 arg1Of and,diluted
R4984 T9528 T9527 arg2Of IκB-α,and
R4985 T9533 T9529 arg2Of Inc.,(
R4986 T9533 T9530 arg1Of Inc.,Cell
R4987 T9533 T9531 arg1Of Inc.,Signaling
R4988 T9533 T9532 arg1Of Inc.,Technology
R4989 T9533 T9534 arg1Of Inc.,","
R4990 T9533 T9538 arg1Of Inc.,","
R4991 T9533 T9539 arg1Of Inc.,USA
R4992 T9535 T9536 arg1Of Beverly,","
R4993 T9536 T9534 arg2Of ",",","
R4994 T9537 T9536 arg2Of MA,","
R4995 T9540 T9529 arg3Of ),(
R4996 T9542 T9524 arg2Of diluted,","
R4997 T9542 T9541 arg1Of diluted,","
R4998 T9543 T9542 arg2Of 1,diluted
R4999 T9545 T9546 arg1Of 1000,in
R5000 T9545 T9551 arg1Of 1000,were
R5001 T9545 T9552 arg2Of 1000,added
R5002 T9545 T9554 arg2Of 1000,incubated
R5003 T9547 T9548 arg1Of 5,%
R5004 T9549 T9546 arg2Of BSA/TBS-T,in
R5005 T9549 T9547 arg1Of BSA/TBS-T,5
R5006 T9552 T9553 arg1Of added,and
R5007 T9553 T9544 arg2Of and,:
R5008 T9553 T9550 arg1Of and,","
R5009 T9553 T9551 arg2Of and,were
R5010 T9553 T9556 arg1Of and,at
R5011 T9554 T9553 arg2Of incubated,and
R5012 T9554 T9555 arg1Of incubated,overnight
R5013 T9557 T9556 arg2Of 4°C,at
R5014 T9559 T9558 arg2Of washing,After
R5015 T9559 T9562 arg1Of washing,with
R5016 T9561 T9559 arg2Of times,washing
R5017 T9561 T9560 arg1Of times,four
R5018 T9563 T9562 arg2Of TBS-T,with
R5019 T9568 T9559 arg1Of antibodies,washing
R5020 T9568 T9565 arg1Of antibodies,horseradish
R5021 T9568 T9566 arg1Of antibodies,peroxidase-conjugated
R5022 T9568 T9567 arg1Of antibodies,secondary
R5023 T9568 T9569 arg1Of antibodies,were
R5024 T9568 T9570 arg2Of antibodies,added
R5025 T9568 T9572 arg2Of antibodies,allowed
R5026 T9568 T9574 arg1Of antibodies,incubate
R5027 T9570 T9571 arg1Of added,and
R5028 T9571 T9558 arg1Of and,After
R5029 T9571 T9564 arg1Of and,","
R5030 T9571 T9569 arg2Of and,were
R5031 T9572 T9571 arg2Of allowed,and
R5032 T9574 T9572 arg3Of incubate,allowed
R5033 T9574 T9573 arg1Of incubate,to
R5034 T9574 T9575 arg1Of incubate,for
R5035 T9574 T9578 arg1Of incubate,at
R5036 T9577 T9575 arg2Of hour,for
R5037 T9577 T9576 arg1Of hour,1
R5038 T9580 T9578 arg2Of temperature,at
R5039 T9580 T9579 arg1Of temperature,room
R5040 T9583 T9581 arg2Of washing,After
R5041 T9583 T9582 arg1Of washing,TBS-T
R5042 T9586 T9585 arg2Of bands,hybridized
R5043 T9586 T9587 arg1Of bands,were
R5044 T9586 T9588 arg2Of bands,detected
R5045 T9588 T9581 arg1Of detected,After
R5046 T9588 T9584 arg1Of detected,","
R5047 T9588 T9587 arg2Of detected,were
R5048 T9588 T9589 arg1Of detected,with
R5049 T9594 T9593 arg2Of ECL,(
R5050 T9595 T9593 arg3Of ),(
R5051 T9597 T9592 arg1Of kit,chemiluminescence
R5052 T9597 T9593 arg1Of kit,(
R5053 T9597 T9596 arg1Of kit,detection
R5054 T9597 T9598 arg1Of kit,and
R5055 T9598 T9589 arg2Of and,with
R5056 T9598 T9590 arg1Of and,the
R5057 T9598 T9591 arg2Of and,enhanced
R5058 T9600 T9598 arg2Of reagents,and
R5059 T9600 T9599 arg1Of reagents,Hyperfilm-ECL
R5060 T9600 T9601 arg1Of reagents,(
R5061 T9603 T9601 arg2Of Pharmacia,(
R5062 T9603 T9602 arg1Of Pharmacia,Amersham
R5063 T9604 T9601 arg3Of ),(