Id |
Subject |
Object |
Predicate |
Lexical cue |
T1674 |
0-10 |
NN |
denotes |
Expression |
T1675 |
11-19 |
NN |
denotes |
analysis |
T1676 |
20-22 |
IN |
denotes |
of |
T1677 |
23-29 |
NN |
denotes |
Mcoln1 |
T1678 |
29-305 |
sentence |
denotes |
Mouse adult multiple tissue and embryonic Northern blots were hybridized using a probe generated from mouse exon 2 (probe 1, Fig. 1B), yielding a band of approximately 2.4 kb (isoform 1), as expected, and a less abundant and unexpected 4.4 kb band (isoform 2) (Figs. 3A &3B). |
T1679 |
30-35 |
NN |
denotes |
Mouse |
T1681 |
36-41 |
JJ |
denotes |
adult |
T1682 |
42-50 |
JJ |
denotes |
multiple |
T1680 |
51-57 |
NN |
denotes |
tissue |
T1684 |
58-61 |
CC |
denotes |
and |
T1685 |
62-71 |
JJ |
denotes |
embryonic |
T1687 |
72-80 |
NNP |
denotes |
Northern |
T1686 |
81-86 |
NNS |
denotes |
blots |
T1688 |
87-91 |
VBD |
denotes |
were |
T1683 |
92-102 |
VBN |
denotes |
hybridized |
T1689 |
103-108 |
VBG |
denotes |
using |
T1690 |
109-110 |
DT |
denotes |
a |
T1691 |
111-116 |
NN |
denotes |
probe |
T1692 |
117-126 |
VBN |
denotes |
generated |
T1693 |
127-131 |
IN |
denotes |
from |
T1694 |
132-137 |
NN |
denotes |
mouse |
T1695 |
138-142 |
NN |
denotes |
exon |
T1696 |
143-144 |
CD |
denotes |
2 |
T1697 |
145-146 |
-LRB- |
denotes |
( |
T1699 |
146-151 |
NN |
denotes |
probe |
T1700 |
152-153 |
CD |
denotes |
1 |
T1701 |
153-155 |
, |
denotes |
, |
T1702 |
155-159 |
NN |
denotes |
Fig. |
T1698 |
160-162 |
NN |
denotes |
1B |
T1703 |
162-163 |
-RRB- |
denotes |
) |
T1704 |
163-165 |
, |
denotes |
, |
T1705 |
165-173 |
VBG |
denotes |
yielding |
T1706 |
174-175 |
DT |
denotes |
a |
T1707 |
176-180 |
NN |
denotes |
band |
T1708 |
181-183 |
IN |
denotes |
of |
T1709 |
184-197 |
RB |
denotes |
approximately |
T1710 |
198-201 |
CD |
denotes |
2.4 |
T1711 |
202-204 |
NNS |
denotes |
kb |
T1712 |
205-206 |
-LRB- |
denotes |
( |
T1713 |
206-213 |
NN |
denotes |
isoform |
T1714 |
214-215 |
CD |
denotes |
1 |
T1715 |
215-216 |
-RRB- |
denotes |
) |
T1716 |
216-218 |
, |
denotes |
, |
T1717 |
218-220 |
IN |
denotes |
as |
T1718 |
221-229 |
VBN |
denotes |
expected |
T1719 |
229-231 |
, |
denotes |
, |
T1720 |
231-234 |
CC |
denotes |
and |
T1721 |
235-236 |
DT |
denotes |
a |
T1723 |
237-241 |
RBR |
denotes |
less |
T1724 |
242-250 |
JJ |
denotes |
abundant |
T1725 |
251-254 |
CC |
denotes |
and |
T1726 |
255-265 |
JJ |
denotes |
unexpected |
T1727 |
266-269 |
CD |
denotes |
4.4 |
T1728 |
270-272 |
NN |
denotes |
kb |
T1722 |
273-277 |
NN |
denotes |
band |
T1729 |
278-279 |
-LRB- |
denotes |
( |
T1730 |
279-286 |
NN |
denotes |
isoform |
T1731 |
287-288 |
CD |
denotes |
2 |
T1732 |
288-289 |
-RRB- |
denotes |
) |
T1733 |
290-291 |
-LRB- |
denotes |
( |
T1735 |
291-296 |
NNS |
denotes |
Figs. |
T1734 |
297-299 |
NN |
denotes |
3A |
T1736 |
300-301 |
CC |
denotes |
& |
T1737 |
301-303 |
NN |
denotes |
3B |
T1738 |
303-304 |
-RRB- |
denotes |
) |
T1739 |
304-305 |
. |
denotes |
. |
T1740 |
305-419 |
sentence |
denotes |
The 2.4 kb band shows ubiquitous but variable expression, with the highest expression in brain, liver and kidney. |
T1741 |
306-309 |
DT |
denotes |
The |
T1743 |
310-313 |
CD |
denotes |
2.4 |
T1744 |
314-316 |
NN |
denotes |
kb |
T1742 |
317-321 |
NN |
denotes |
band |
T1745 |
322-327 |
VBZ |
denotes |
shows |
T1746 |
328-338 |
JJ |
denotes |
ubiquitous |
T1748 |
339-342 |
CC |
denotes |
but |
T1749 |
343-351 |
JJ |
denotes |
variable |
T1747 |
352-362 |
NN |
denotes |
expression |
T1750 |
362-364 |
, |
denotes |
, |
T1751 |
364-368 |
IN |
denotes |
with |
T1753 |
369-372 |
DT |
denotes |
the |
T1754 |
373-380 |
JJS |
denotes |
highest |
T1752 |
381-391 |
NN |
denotes |
expression |
T1755 |
392-394 |
IN |
denotes |
in |
T1756 |
395-400 |
NN |
denotes |
brain |
T1757 |
400-402 |
, |
denotes |
, |
T1758 |
402-407 |
NN |
denotes |
liver |
T1759 |
408-411 |
CC |
denotes |
and |
T1760 |
412-418 |
NN |
denotes |
kidney |
T1761 |
418-419 |
. |
denotes |
. |
T1762 |
419-520 |
sentence |
denotes |
The fetal tissue blot shows decreasing levels of the 2.4 kb message with increasing gestational age. |
T1763 |
420-423 |
DT |
denotes |
The |
T1765 |
424-429 |
JJ |
denotes |
fetal |
T1766 |
430-436 |
NN |
denotes |
tissue |
T1764 |
437-441 |
NN |
denotes |
blot |
T1767 |
442-447 |
VBZ |
denotes |
shows |
T1768 |
448-458 |
VBG |
denotes |
decreasing |
T1769 |
459-465 |
NNS |
denotes |
levels |
T1770 |
466-468 |
IN |
denotes |
of |
T1771 |
469-472 |
DT |
denotes |
the |
T1773 |
473-476 |
CD |
denotes |
2.4 |
T1774 |
477-479 |
NN |
denotes |
kb |
T1772 |
480-487 |
NN |
denotes |
message |
T1775 |
488-492 |
IN |
denotes |
with |
T1776 |
493-503 |
VBG |
denotes |
increasing |
T1778 |
504-515 |
JJ |
denotes |
gestational |
T1777 |
516-519 |
NN |
denotes |
age |
T1779 |
519-520 |
. |
denotes |
. |
T1780 |
520-784 |
sentence |
denotes |
Since the human MCOLN1 gene encodes a single transcript [7-9], we carried out additional hybridizations with probes generated from various regions of the coding sequence and 3' UTR, and all probes identified the same two transcripts in the mouse (data not shown). |
T1781 |
521-526 |
IN |
denotes |
Since |
T1783 |
527-530 |
DT |
denotes |
the |
T1785 |
531-536 |
JJ |
denotes |
human |
T1786 |
537-543 |
NN |
denotes |
MCOLN1 |
T1784 |
544-548 |
NN |
denotes |
gene |
T1782 |
549-556 |
VBZ |
denotes |
encodes |
T1788 |
557-558 |
DT |
denotes |
a |
T1790 |
559-565 |
JJ |
denotes |
single |
T1789 |
566-576 |
NN |
denotes |
transcript |
T1791 |
577-578 |
-LRB- |
denotes |
[ |
T1792 |
578-579 |
CD |
denotes |
7 |
T1793 |
579-580 |
SYM |
denotes |
- |
T1794 |
580-581 |
CD |
denotes |
9 |
T1795 |
581-582 |
-RRB- |
denotes |
] |
T1796 |
582-584 |
, |
denotes |
, |
T1797 |
584-586 |
PRP |
denotes |
we |
T1787 |
587-594 |
VBD |
denotes |
carried |
T1798 |
595-598 |
RP |
denotes |
out |
T1799 |
599-609 |
JJ |
denotes |
additional |
T1800 |
610-624 |
NNS |
denotes |
hybridizations |
T1801 |
625-629 |
IN |
denotes |
with |
T1802 |
630-636 |
NNS |
denotes |
probes |
T1803 |
637-646 |
VBN |
denotes |
generated |
T1804 |
647-651 |
IN |
denotes |
from |
T1805 |
652-659 |
JJ |
denotes |
various |
T1806 |
660-667 |
NNS |
denotes |
regions |
T1807 |
668-670 |
IN |
denotes |
of |
T1808 |
671-674 |
DT |
denotes |
the |
T1810 |
675-681 |
VBG |
denotes |
coding |
T1809 |
682-690 |
NN |
denotes |
sequence |
T1811 |
691-694 |
CC |
denotes |
and |
T1812 |
695-696 |
CD |
denotes |
3 |
T1814 |
696-697 |
SYM |
denotes |
' |
T1813 |
698-701 |
NN |
denotes |
UTR |
T1815 |
701-703 |
, |
denotes |
, |
T1816 |
703-706 |
CC |
denotes |
and |
T1817 |
707-710 |
DT |
denotes |
all |
T1818 |
711-717 |
NNS |
denotes |
probes |
T1819 |
718-728 |
VBD |
denotes |
identified |
T1820 |
729-732 |
DT |
denotes |
the |
T1822 |
733-737 |
JJ |
denotes |
same |
T1823 |
738-741 |
CD |
denotes |
two |
T1821 |
742-753 |
NNS |
denotes |
transcripts |
T1824 |
754-756 |
IN |
denotes |
in |
T1825 |
757-760 |
DT |
denotes |
the |
T1826 |
761-766 |
NN |
denotes |
mouse |
T1827 |
767-768 |
-LRB- |
denotes |
( |
T1829 |
768-772 |
NNS |
denotes |
data |
T1830 |
773-776 |
RB |
denotes |
not |
T1828 |
777-782 |
VBN |
denotes |
shown |
T1831 |
782-783 |
-RRB- |
denotes |
) |
T1832 |
783-784 |
. |
denotes |
. |
T1833 |
784-900 |
sentence |
denotes |
In order to verify the presence of a single mouse locus, we hybridized a mouse Southern blot with the exon 2 probe. |
T1834 |
785-787 |
IN |
denotes |
In |
T1836 |
788-793 |
NN |
denotes |
order |
T1837 |
794-796 |
TO |
denotes |
to |
T1838 |
797-803 |
VB |
denotes |
verify |
T1839 |
804-807 |
DT |
denotes |
the |
T1840 |
808-816 |
NN |
denotes |
presence |
T1841 |
817-819 |
IN |
denotes |
of |
T1842 |
820-821 |
DT |
denotes |
a |
T1844 |
822-828 |
JJ |
denotes |
single |
T1845 |
829-834 |
NN |
denotes |
mouse |
T1843 |
835-840 |
NN |
denotes |
locus |
T1846 |
840-842 |
, |
denotes |
, |
T1847 |
842-844 |
PRP |
denotes |
we |
T1835 |
845-855 |
VBD |
denotes |
hybridized |
T1848 |
856-857 |
DT |
denotes |
a |
T1850 |
858-863 |
NN |
denotes |
mouse |
T1851 |
864-872 |
NN |
denotes |
Southern |
T1849 |
873-877 |
NN |
denotes |
blot |
T1852 |
878-882 |
IN |
denotes |
with |
T1853 |
883-886 |
DT |
denotes |
the |
T1855 |
887-891 |
NN |
denotes |
exon |
T1856 |
892-893 |
CD |
denotes |
2 |
T1854 |
894-899 |
NN |
denotes |
probe |
T1857 |
899-900 |
. |
denotes |
. |
T1858 |
900-1038 |
sentence |
denotes |
Four different restriction enzymes were used, and only the expected size bands for the chromosome 8 locus were detected (data not shown). |
T1859 |
901-905 |
CD |
denotes |
Four |
T1861 |
906-915 |
JJ |
denotes |
different |
T1862 |
916-927 |
NN |
denotes |
restriction |
T1860 |
928-935 |
NNS |
denotes |
enzymes |
T1864 |
936-940 |
VBD |
denotes |
were |
T1863 |
941-945 |
VBN |
denotes |
used |
T1865 |
945-947 |
, |
denotes |
, |
T1866 |
947-950 |
CC |
denotes |
and |
T1867 |
951-955 |
RB |
denotes |
only |
T1869 |
956-959 |
DT |
denotes |
the |
T1870 |
960-968 |
VBN |
denotes |
expected |
T1871 |
969-973 |
NN |
denotes |
size |
T1868 |
974-979 |
NNS |
denotes |
bands |
T1873 |
980-983 |
IN |
denotes |
for |
T1874 |
984-987 |
DT |
denotes |
the |
T1876 |
988-998 |
NN |
denotes |
chromosome |
T1877 |
999-1000 |
CD |
denotes |
8 |
T1875 |
1001-1006 |
NN |
denotes |
locus |
T1878 |
1007-1011 |
VBD |
denotes |
were |
T1872 |
1012-1020 |
VBN |
denotes |
detected |
T1879 |
1021-1022 |
-LRB- |
denotes |
( |
T1881 |
1022-1026 |
NNS |
denotes |
data |
T1882 |
1027-1030 |
RB |
denotes |
not |
T1880 |
1031-1036 |
VBN |
denotes |
shown |
T1883 |
1036-1037 |
-RRB- |
denotes |
) |
T1884 |
1037-1038 |
. |
denotes |
. |
T4522 |
1049-1057 |
NNP |
denotes |
Northern |
T4523 |
1058-1066 |
NN |
denotes |
analysis |
T4524 |
1067-1069 |
IN |
denotes |
of |
T4525 |
1070-1076 |
NN |
denotes |
Mcoln1 |
T4526 |
1076-1077 |
. |
denotes |
. |
T4527 |
1077-1231 |
sentence |
denotes |
(A) Mouse fetal tissue Northern and (B) mouse multiple tissue Northern blots (Clontech) hybridized with a probe generated from exon 2 (probe 1, Fig. 1B). |
T4528 |
1078-1079 |
-LRB- |
denotes |
( |
T4529 |
1079-1080 |
LS |
denotes |
A |
T4531 |
1080-1081 |
-RRB- |
denotes |
) |
T4532 |
1082-1087 |
NN |
denotes |
Mouse |
T4534 |
1088-1093 |
JJ |
denotes |
fetal |
T4533 |
1094-1100 |
NN |
denotes |
tissue |
T4530 |
1101-1109 |
NN |
denotes |
Northern |
T4536 |
1110-1113 |
CC |
denotes |
and |
T4537 |
1114-1115 |
-LRB- |
denotes |
( |
T4538 |
1115-1116 |
LS |
denotes |
B |
T4540 |
1116-1117 |
-RRB- |
denotes |
) |
T4541 |
1118-1123 |
NN |
denotes |
mouse |
T4543 |
1124-1132 |
JJ |
denotes |
multiple |
T4542 |
1133-1139 |
NN |
denotes |
tissue |
T4539 |
1140-1148 |
NN |
denotes |
Northern |
T4535 |
1149-1154 |
NNS |
denotes |
blots |
T4545 |
1155-1156 |
-LRB- |
denotes |
( |
T4546 |
1156-1164 |
NN |
denotes |
Clontech |
T4547 |
1164-1165 |
-RRB- |
denotes |
) |
T4544 |
1166-1176 |
VBD |
denotes |
hybridized |
T4548 |
1177-1181 |
IN |
denotes |
with |
T4549 |
1182-1183 |
DT |
denotes |
a |
T4550 |
1184-1189 |
NN |
denotes |
probe |
T4551 |
1190-1199 |
VBN |
denotes |
generated |
T4552 |
1200-1204 |
IN |
denotes |
from |
T4553 |
1205-1209 |
NN |
denotes |
exon |
T4554 |
1210-1211 |
CD |
denotes |
2 |
T4555 |
1212-1213 |
-LRB- |
denotes |
( |
T4557 |
1213-1218 |
NN |
denotes |
probe |
T4558 |
1219-1220 |
CD |
denotes |
1 |
T4559 |
1220-1222 |
, |
denotes |
, |
T4560 |
1222-1226 |
NN |
denotes |
Fig. |
T4556 |
1227-1229 |
NN |
denotes |
1B |
T4561 |
1229-1230 |
-RRB- |
denotes |
) |
T4562 |
1230-1231 |
. |
denotes |
. |
T4563 |
1231-1360 |
sentence |
denotes |
(C) Hybridization of the multiple tissue blot with a probe from the alternatively spliced segment of exon 13 (probe 2, Fig. 1B). |
T4564 |
1232-1233 |
-LRB- |
denotes |
( |
T4565 |
1233-1234 |
LS |
denotes |
C |
T4567 |
1234-1235 |
-RRB- |
denotes |
) |
T4566 |
1236-1249 |
NN |
denotes |
Hybridization |
T4568 |
1250-1252 |
IN |
denotes |
of |
T4569 |
1253-1256 |
DT |
denotes |
the |
T4571 |
1257-1265 |
JJ |
denotes |
multiple |
T4572 |
1266-1272 |
NN |
denotes |
tissue |
T4570 |
1273-1277 |
NN |
denotes |
blot |
T4573 |
1278-1282 |
IN |
denotes |
with |
T4574 |
1283-1284 |
DT |
denotes |
a |
T4575 |
1285-1290 |
NN |
denotes |
probe |
T4576 |
1291-1295 |
IN |
denotes |
from |
T4577 |
1296-1299 |
DT |
denotes |
the |
T4579 |
1300-1313 |
RB |
denotes |
alternatively |
T4580 |
1314-1321 |
VBN |
denotes |
spliced |
T4578 |
1322-1329 |
NN |
denotes |
segment |
T4581 |
1330-1332 |
IN |
denotes |
of |
T4582 |
1333-1337 |
NN |
denotes |
exon |
T4583 |
1338-1340 |
CD |
denotes |
13 |
T4584 |
1341-1342 |
-LRB- |
denotes |
( |
T4586 |
1342-1347 |
NN |
denotes |
probe |
T4587 |
1348-1349 |
CD |
denotes |
2 |
T4588 |
1349-1351 |
, |
denotes |
, |
T4589 |
1351-1355 |
NN |
denotes |
Fig. |
T4585 |
1356-1358 |
NN |
denotes |
1B |
T4590 |
1358-1359 |
-RRB- |
denotes |
) |
T4591 |
1359-1360 |
. |
denotes |
. |
T4592 |
1360-1416 |
sentence |
denotes |
β-Actin control hybridization is shown below each blot. |
T4593 |
1361-1362 |
NN |
denotes |
β |
T4595 |
1362-1363 |
HYPH |
denotes |
- |
T4594 |
1363-1368 |
NN |
denotes |
Actin |
T4597 |
1369-1376 |
NN |
denotes |
control |
T4596 |
1377-1390 |
NN |
denotes |
hybridization |
T4599 |
1391-1393 |
VBZ |
denotes |
is |
T4598 |
1394-1399 |
VBN |
denotes |
shown |
T4600 |
1400-1405 |
IN |
denotes |
below |
T4601 |
1406-1410 |
DT |
denotes |
each |
T4602 |
1411-1415 |
NN |
denotes |
blot |
T4603 |
1415-1416 |
. |
denotes |
. |
R1000 |
T1679 |
T1680 |
nmod |
Mouse,tissue |
R1001 |
T1680 |
T1683 |
nsubjpass |
tissue,hybridized |
R1002 |
T1681 |
T1680 |
amod |
adult,tissue |
R1003 |
T1682 |
T1680 |
amod |
multiple,tissue |
R1004 |
T1684 |
T1680 |
cc |
and,tissue |
R1005 |
T1685 |
T1686 |
amod |
embryonic,blots |
R1006 |
T1686 |
T1680 |
conj |
blots,tissue |
R1007 |
T1687 |
T1686 |
compound |
Northern,blots |
R1008 |
T1688 |
T1683 |
auxpass |
were,hybridized |
R1009 |
T1689 |
T1683 |
advcl |
using,hybridized |
R1010 |
T1690 |
T1691 |
det |
a,probe |
R1011 |
T1691 |
T1689 |
dobj |
probe,using |
R1012 |
T1692 |
T1691 |
acl |
generated,probe |
R1013 |
T1693 |
T1692 |
prep |
from,generated |
R1014 |
T1694 |
T1695 |
compound |
mouse,exon |
R1015 |
T1695 |
T1693 |
pobj |
exon,from |
R1016 |
T1696 |
T1695 |
nummod |
2,exon |
R1017 |
T1697 |
T1698 |
punct |
(,1B |
R1018 |
T1698 |
T1691 |
parataxis |
1B,probe |
R1019 |
T1699 |
T1698 |
dep |
probe,1B |
R1020 |
T1700 |
T1699 |
nummod |
1,probe |
R1021 |
T1701 |
T1698 |
punct |
", ",1B |
R1022 |
T1702 |
T1698 |
compound |
Fig.,1B |
R1023 |
T1703 |
T1698 |
punct |
),1B |
R1024 |
T1704 |
T1683 |
punct |
", ",hybridized |
R1025 |
T1705 |
T1683 |
advcl |
yielding,hybridized |
R1026 |
T1706 |
T1707 |
det |
a,band |
R1027 |
T1707 |
T1705 |
dobj |
band,yielding |
R1028 |
T1708 |
T1707 |
prep |
of,band |
R1029 |
T1709 |
T1710 |
advmod |
approximately,2.4 |
R1030 |
T1710 |
T1711 |
nummod |
2.4,kb |
R1031 |
T1711 |
T1708 |
pobj |
kb,of |
R1032 |
T1712 |
T1707 |
punct |
(,band |
R1033 |
T1713 |
T1707 |
appos |
isoform,band |
R1034 |
T1714 |
T1713 |
nummod |
1,isoform |
R1035 |
T1715 |
T1707 |
punct |
),band |
R1036 |
T1716 |
T1707 |
punct |
", ",band |
R1037 |
T1717 |
T1718 |
mark |
as,expected |
R1038 |
T1718 |
T1707 |
advcl |
expected,band |
R1039 |
T1719 |
T1707 |
punct |
", ",band |
R1040 |
T1720 |
T1707 |
cc |
and,band |
R1041 |
T1721 |
T1722 |
det |
a,band |
R1042 |
T1722 |
T1707 |
conj |
band,band |
R1043 |
T1723 |
T1724 |
advmod |
less,abundant |
R1044 |
T1724 |
T1722 |
amod |
abundant,band |
R1045 |
T1725 |
T1724 |
cc |
and,abundant |
R1046 |
T1726 |
T1724 |
conj |
unexpected,abundant |
R1047 |
T1727 |
T1728 |
nummod |
4.4,kb |
R1048 |
T1728 |
T1722 |
compound |
kb,band |
R1049 |
T1729 |
T1722 |
punct |
(,band |
R1050 |
T1730 |
T1722 |
appos |
isoform,band |
R1051 |
T1731 |
T1730 |
nummod |
2,isoform |
R1052 |
T1732 |
T1705 |
punct |
),yielding |
R1053 |
T1733 |
T1734 |
punct |
(,3A |
R1054 |
T1734 |
T1705 |
parataxis |
3A,yielding |
R1055 |
T1735 |
T1734 |
compound |
Figs.,3A |
R1056 |
T1736 |
T1734 |
cc |
&,3A |
R1057 |
T1737 |
T1734 |
conj |
3B,3A |
R1058 |
T1738 |
T1734 |
punct |
),3A |
R1059 |
T1739 |
T1683 |
punct |
.,hybridized |
R1060 |
T1741 |
T1742 |
det |
The,band |
R1061 |
T1742 |
T1745 |
nsubj |
band,shows |
R1062 |
T1743 |
T1744 |
nummod |
2.4,kb |
R1063 |
T1744 |
T1742 |
compound |
kb,band |
R1064 |
T1746 |
T1747 |
amod |
ubiquitous,expression |
R1065 |
T1747 |
T1745 |
dobj |
expression,shows |
R1066 |
T1748 |
T1746 |
cc |
but,ubiquitous |
R1067 |
T1749 |
T1746 |
conj |
variable,ubiquitous |
R1068 |
T1750 |
T1745 |
punct |
", ",shows |
R1069 |
T1751 |
T1752 |
mark |
with,expression |
R1070 |
T1752 |
T1745 |
advcl |
expression,shows |
R1071 |
T1753 |
T1752 |
det |
the,expression |
R1072 |
T1754 |
T1752 |
amod |
highest,expression |
R1073 |
T1755 |
T1752 |
prep |
in,expression |
R1074 |
T1756 |
T1755 |
pobj |
brain,in |
R1075 |
T1757 |
T1756 |
punct |
", ",brain |
R1076 |
T1758 |
T1756 |
conj |
liver,brain |
R1077 |
T1759 |
T1758 |
cc |
and,liver |
R1078 |
T1760 |
T1758 |
conj |
kidney,liver |
R1079 |
T1761 |
T1745 |
punct |
.,shows |
R1080 |
T1763 |
T1764 |
det |
The,blot |
R1081 |
T1764 |
T1767 |
nsubj |
blot,shows |
R1082 |
T1765 |
T1766 |
amod |
fetal,tissue |
R1083 |
T1766 |
T1764 |
compound |
tissue,blot |
R1084 |
T1768 |
T1769 |
amod |
decreasing,levels |
R1085 |
T1769 |
T1767 |
dobj |
levels,shows |
R1086 |
T1770 |
T1769 |
prep |
of,levels |
R1087 |
T1771 |
T1772 |
det |
the,message |
R1088 |
T1772 |
T1770 |
pobj |
message,of |
R1089 |
T1773 |
T1774 |
nummod |
2.4,kb |
R1090 |
T1774 |
T1772 |
compound |
kb,message |
R1091 |
T1775 |
T1769 |
prep |
with,levels |
R1092 |
T1776 |
T1777 |
amod |
increasing,age |
R1093 |
T1777 |
T1775 |
pobj |
age,with |
R1094 |
T1778 |
T1777 |
amod |
gestational,age |
R1095 |
T1779 |
T1767 |
punct |
.,shows |
R1096 |
T1781 |
T1782 |
mark |
Since,encodes |
R1097 |
T1782 |
T1787 |
advcl |
encodes,carried |
R1098 |
T1783 |
T1784 |
det |
the,gene |
R1099 |
T1784 |
T1782 |
nsubj |
gene,encodes |
R1100 |
T1785 |
T1784 |
amod |
human,gene |
R1101 |
T1786 |
T1784 |
compound |
MCOLN1,gene |
R1102 |
T1788 |
T1789 |
det |
a,transcript |
R1103 |
T1789 |
T1782 |
dobj |
transcript,encodes |
R1104 |
T1790 |
T1789 |
amod |
single,transcript |
R1105 |
T1791 |
T1792 |
punct |
[,7 |
R1106 |
T1792 |
T1782 |
parataxis |
7,encodes |
R1107 |
T1793 |
T1794 |
punct |
-,9 |
R1108 |
T1794 |
T1792 |
prep |
9,7 |
R1109 |
T1795 |
T1792 |
punct |
],7 |
R1110 |
T1796 |
T1787 |
punct |
", ",carried |
R1111 |
T1797 |
T1787 |
nsubj |
we,carried |
R1112 |
T1798 |
T1787 |
prt |
out,carried |
R1113 |
T1799 |
T1800 |
amod |
additional,hybridizations |
R1114 |
T1800 |
T1787 |
dobj |
hybridizations,carried |
R1115 |
T1801 |
T1787 |
prep |
with,carried |
R1116 |
T1802 |
T1801 |
pobj |
probes,with |
R1117 |
T1803 |
T1802 |
acl |
generated,probes |
R1118 |
T1804 |
T1803 |
prep |
from,generated |
R1119 |
T1805 |
T1806 |
amod |
various,regions |
R1120 |
T1806 |
T1804 |
pobj |
regions,from |
R1121 |
T1807 |
T1806 |
prep |
of,regions |
R1122 |
T1808 |
T1809 |
det |
the,sequence |
R1123 |
T1809 |
T1807 |
pobj |
sequence,of |
R1124 |
T1810 |
T1809 |
amod |
coding,sequence |
R1125 |
T1811 |
T1809 |
cc |
and,sequence |
R1126 |
T1812 |
T1813 |
nummod |
3,UTR |
R1127 |
T1813 |
T1809 |
conj |
UTR,sequence |
R1128 |
T1814 |
T1812 |
punct |
',3 |
R1129 |
T1815 |
T1787 |
punct |
", ",carried |
R1130 |
T1816 |
T1787 |
cc |
and,carried |
R1131 |
T1817 |
T1818 |
det |
all,probes |
R1132 |
T1818 |
T1819 |
nsubj |
probes,identified |
R1133 |
T1819 |
T1787 |
conj |
identified,carried |
R1134 |
T1820 |
T1821 |
det |
the,transcripts |
R1135 |
T1821 |
T1819 |
dobj |
transcripts,identified |
R1136 |
T1822 |
T1821 |
amod |
same,transcripts |
R1137 |
T1823 |
T1821 |
nummod |
two,transcripts |
R1138 |
T1824 |
T1819 |
prep |
in,identified |
R1139 |
T1825 |
T1826 |
det |
the,mouse |
R1140 |
T1826 |
T1824 |
pobj |
mouse,in |
R1141 |
T1827 |
T1828 |
punct |
(,shown |
R1142 |
T1828 |
T1819 |
parataxis |
shown,identified |
R1143 |
T1829 |
T1828 |
nsubj |
data,shown |
R1144 |
T1830 |
T1828 |
neg |
not,shown |
R1145 |
T1831 |
T1828 |
punct |
),shown |
R1146 |
T1832 |
T1819 |
punct |
.,identified |
R1147 |
T1834 |
T1835 |
prep |
In,hybridized |
R1148 |
T1836 |
T1834 |
pobj |
order,In |
R1149 |
T1837 |
T1838 |
aux |
to,verify |
R1150 |
T1838 |
T1836 |
acl |
verify,order |
R1151 |
T1839 |
T1840 |
det |
the,presence |
R1152 |
T1840 |
T1838 |
dobj |
presence,verify |
R1153 |
T1841 |
T1840 |
prep |
of,presence |
R1154 |
T1842 |
T1843 |
det |
a,locus |
R1155 |
T1843 |
T1841 |
pobj |
locus,of |
R1156 |
T1844 |
T1843 |
amod |
single,locus |
R1157 |
T1845 |
T1843 |
compound |
mouse,locus |
R1158 |
T1846 |
T1835 |
punct |
", ",hybridized |
R1159 |
T1847 |
T1835 |
nsubj |
we,hybridized |
R1160 |
T1848 |
T1849 |
det |
a,blot |
R1161 |
T1849 |
T1835 |
dobj |
blot,hybridized |
R1162 |
T1850 |
T1849 |
compound |
mouse,blot |
R1163 |
T1851 |
T1849 |
compound |
Southern,blot |
R1164 |
T1852 |
T1835 |
prep |
with,hybridized |
R1165 |
T1853 |
T1854 |
det |
the,probe |
R1166 |
T1854 |
T1852 |
pobj |
probe,with |
R1167 |
T1855 |
T1854 |
nmod |
exon,probe |
R1168 |
T1856 |
T1855 |
nummod |
2,exon |
R1169 |
T1857 |
T1835 |
punct |
.,hybridized |
R1170 |
T1859 |
T1860 |
nummod |
Four,enzymes |
R1171 |
T1860 |
T1863 |
nsubjpass |
enzymes,used |
R1172 |
T1861 |
T1860 |
amod |
different,enzymes |
R1173 |
T1862 |
T1860 |
compound |
restriction,enzymes |
R1174 |
T1864 |
T1863 |
auxpass |
were,used |
R1175 |
T1865 |
T1863 |
punct |
", ",used |
R1176 |
T1866 |
T1863 |
cc |
and,used |
R1177 |
T1867 |
T1868 |
advmod |
only,bands |
R1178 |
T1868 |
T1872 |
nsubjpass |
bands,detected |
R1179 |
T1869 |
T1868 |
det |
the,bands |
R1180 |
T1870 |
T1871 |
amod |
expected,size |
R1181 |
T1871 |
T1868 |
compound |
size,bands |
R1182 |
T1872 |
T1863 |
conj |
detected,used |
R1183 |
T1873 |
T1868 |
prep |
for,bands |
R1184 |
T1874 |
T1875 |
det |
the,locus |
R1185 |
T1875 |
T1873 |
pobj |
locus,for |
R1186 |
T1876 |
T1875 |
nmod |
chromosome,locus |
R1187 |
T1877 |
T1876 |
nummod |
8,chromosome |
R1188 |
T1878 |
T1872 |
auxpass |
were,detected |
R1189 |
T1879 |
T1880 |
punct |
(,shown |
R1190 |
T1880 |
T1872 |
parataxis |
shown,detected |
R1191 |
T1881 |
T1880 |
nsubj |
data,shown |
R1192 |
T1882 |
T1880 |
neg |
not,shown |
R1193 |
T1883 |
T1880 |
punct |
),shown |
R1194 |
T1884 |
T1872 |
punct |
.,detected |
R2809 |
T4522 |
T4523 |
compound |
Northern,analysis |
R2810 |
T4524 |
T4523 |
prep |
of,analysis |
R2811 |
T4525 |
T4524 |
pobj |
Mcoln1,of |
R2812 |
T4526 |
T4523 |
punct |
.,analysis |
R2813 |
T4528 |
T4529 |
punct |
(,A |
R2814 |
T4529 |
T4530 |
meta |
A,Northern |
R2815 |
T4530 |
T4535 |
nmod |
Northern,blots |
R2816 |
T4531 |
T4529 |
punct |
),A |
R2817 |
T4532 |
T4533 |
nmod |
Mouse,tissue |
R2818 |
T4533 |
T4530 |
nmod |
tissue,Northern |
R2819 |
T4534 |
T4533 |
amod |
fetal,tissue |
R2820 |
T4535 |
T4544 |
nsubj |
blots,hybridized |
R2821 |
T4536 |
T4530 |
cc |
and,Northern |
R2822 |
T4537 |
T4538 |
punct |
(,B |
R2823 |
T4538 |
T4539 |
meta |
B,Northern |
R2824 |
T4539 |
T4530 |
conj |
Northern,Northern |
R2825 |
T4540 |
T4538 |
punct |
),B |
R2826 |
T4541 |
T4542 |
nmod |
mouse,tissue |
R2827 |
T4542 |
T4539 |
compound |
tissue,Northern |
R2828 |
T4543 |
T4542 |
amod |
multiple,tissue |
R2829 |
T4545 |
T4546 |
punct |
(,Clontech |
R2830 |
T4546 |
T4535 |
parataxis |
Clontech,blots |
R2831 |
T4547 |
T4546 |
punct |
),Clontech |
R2832 |
T4548 |
T4544 |
prep |
with,hybridized |
R2833 |
T4549 |
T4550 |
det |
a,probe |
R2834 |
T4550 |
T4548 |
pobj |
probe,with |
R2835 |
T4551 |
T4550 |
acl |
generated,probe |
R2836 |
T4552 |
T4551 |
prep |
from,generated |
R2837 |
T4553 |
T4552 |
pobj |
exon,from |
R2838 |
T4554 |
T4553 |
nummod |
2,exon |
R2839 |
T4555 |
T4556 |
punct |
(,1B |
R2840 |
T4556 |
T4544 |
parataxis |
1B,hybridized |
R2841 |
T4557 |
T4556 |
dep |
probe,1B |
R2842 |
T4558 |
T4557 |
nummod |
1,probe |
R2843 |
T4559 |
T4556 |
punct |
", ",1B |
R2844 |
T4560 |
T4556 |
compound |
Fig.,1B |
R2845 |
T4561 |
T4556 |
punct |
),1B |
R2846 |
T4562 |
T4544 |
punct |
.,hybridized |
R2847 |
T4564 |
T4565 |
punct |
(,C |
R2848 |
T4565 |
T4566 |
meta |
C,Hybridization |
R2849 |
T4567 |
T4565 |
punct |
),C |
R2850 |
T4568 |
T4566 |
prep |
of,Hybridization |
R2851 |
T4569 |
T4570 |
det |
the,blot |
R2852 |
T4570 |
T4568 |
pobj |
blot,of |
R2853 |
T4571 |
T4572 |
amod |
multiple,tissue |
R2854 |
T4572 |
T4570 |
compound |
tissue,blot |
R2855 |
T4573 |
T4566 |
prep |
with,Hybridization |
R2856 |
T4574 |
T4575 |
det |
a,probe |
R2857 |
T4575 |
T4573 |
pobj |
probe,with |
R2858 |
T4576 |
T4575 |
prep |
from,probe |
R2859 |
T4577 |
T4578 |
det |
the,segment |
R2860 |
T4578 |
T4576 |
pobj |
segment,from |
R2861 |
T4579 |
T4580 |
advmod |
alternatively,spliced |
R2862 |
T4580 |
T4578 |
amod |
spliced,segment |
R2863 |
T4581 |
T4578 |
prep |
of,segment |
R2864 |
T4582 |
T4581 |
pobj |
exon,of |
R2865 |
T4583 |
T4582 |
nummod |
13,exon |
R2866 |
T4584 |
T4585 |
punct |
(,1B |
R2867 |
T4585 |
T4566 |
parataxis |
1B,Hybridization |
R2868 |
T4586 |
T4585 |
dep |
probe,1B |
R2869 |
T4587 |
T4586 |
nummod |
2,probe |
R2870 |
T4588 |
T4585 |
punct |
", ",1B |
R2871 |
T4589 |
T4585 |
compound |
Fig.,1B |
R2872 |
T4590 |
T4585 |
punct |
),1B |
R2873 |
T4591 |
T4566 |
punct |
.,Hybridization |
R2874 |
T4593 |
T4594 |
compound |
β,Actin |
R2875 |
T4594 |
T4596 |
compound |
Actin,hybridization |
R2876 |
T4595 |
T4594 |
punct |
-,Actin |
R2877 |
T4596 |
T4598 |
nsubjpass |
hybridization,shown |
R2878 |
T4597 |
T4596 |
compound |
control,hybridization |
R2879 |
T4599 |
T4598 |
auxpass |
is,shown |
R2880 |
T4600 |
T4598 |
prep |
below,shown |
R2881 |
T4601 |
T4602 |
det |
each,blot |
R2882 |
T4602 |
T4600 |
pobj |
blot,below |
R2883 |
T4603 |
T4598 |
punct |
.,shown |
R997 |
T1674 |
T1675 |
compound |
Expression,analysis |
R998 |
T1676 |
T1675 |
prep |
of,analysis |
R999 |
T1677 |
T1676 |
pobj |
Mcoln1,of |