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PMC:88885 / 14986-15548 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T3835 0-8 NNP denotes Southern
T3836 9-13 NN denotes Blot
T3837 14-22 NN denotes Analysis
T3838 22-95 sentence denotes Total mouse genomic DNA was digested using EcoRI, BamHI, PstI, and XbaI.
T3839 23-28 JJ denotes Total
T3841 29-34 NN denotes mouse
T3842 35-42 JJ denotes genomic
T3840 43-46 NN denotes DNA
T3844 47-50 VBD denotes was
T3843 51-59 VBN denotes digested
T3845 60-65 VBG denotes using
T3846 66-71 NN denotes EcoRI
T3847 71-73 , denotes ,
T3848 73-78 NN denotes BamHI
T3849 78-80 , denotes ,
T3850 80-84 NN denotes PstI
T3851 84-86 , denotes ,
T3852 86-89 CC denotes and
T3853 90-94 NN denotes XbaI
T3854 94-95 . denotes .
T3855 95-262 sentence denotes The digests were electrophoresed on a 1% agarose gel at 60V overnight and were transferred onto a Hybond N+ membrane from Amersham Pharmacia Biotech (Piscataway, NJ).
T3856 96-99 DT denotes The
T3857 100-107 NNS denotes digests
T3859 108-112 VBD denotes were
T3858 113-128 VBN denotes electrophoresed
T3860 129-131 IN denotes on
T3861 132-133 DT denotes a
T3863 134-135 CD denotes 1
T3864 135-136 NN denotes %
T3865 137-144 NN denotes agarose
T3862 145-148 NN denotes gel
T3866 149-151 IN denotes at
T3867 152-155 NN denotes 60V
T3868 156-165 RB denotes overnight
T3869 166-169 CC denotes and
T3870 170-174 VBD denotes were
T3871 175-186 VBN denotes transferred
T3872 187-191 IN denotes onto
T3873 192-193 DT denotes a
T3875 194-200 NN denotes Hybond
T3876 201-203 JJ denotes N+
T3874 204-212 NN denotes membrane
T3877 213-217 IN denotes from
T3878 218-226 NNP denotes Amersham
T3880 227-236 NNP denotes Pharmacia
T3879 237-244 NNP denotes Biotech
T3881 245-246 -LRB- denotes (
T3882 246-256 NNP denotes Piscataway
T3883 256-258 , denotes ,
T3884 258-260 NNP denotes NJ
T3885 260-261 -RRB- denotes )
T3886 261-262 . denotes .
T3887 262-491 sentence denotes A 32P-dATP labeled PCR fragment of the Mcoln1 coding region corresponding to exon 2 was used as a probe (primers 5'-CCCCACAGAAGAGGAAGAC-3' (forward) and 5'-AGATCTTGACCACCTGCAG-3' (reverse) with an annealing temperature of 59°C).
T3888 263-264 DT denotes A
T3890 265-268 NN denotes 32P
T3892 268-269 HYPH denotes -
T3891 269-273 NN denotes dATP
T3893 274-281 JJ denotes labeled
T3894 282-285 NN denotes PCR
T3889 286-294 NN denotes fragment
T3896 295-297 IN denotes of
T3897 298-301 DT denotes the
T3899 302-308 NN denotes Mcoln1
T3900 309-315 NN denotes coding
T3898 316-322 NN denotes region
T3901 323-336 VBG denotes corresponding
T3902 337-339 IN denotes to
T3903 340-344 NN denotes exon
T3904 345-346 CD denotes 2
T3905 347-350 VBD denotes was
T3895 351-355 VBN denotes used
T3906 356-358 IN denotes as
T3907 359-360 DT denotes a
T3908 361-366 NN denotes probe
T3909 367-368 -LRB- denotes (
T3911 368-375 NNS denotes primers
T3912 376-377 CD denotes 5
T3913 377-378 SYM denotes '
T3914 378-379 HYPH denotes -
T3915 379-398 NN denotes CCCCACAGAAGAGGAAGAC
T3916 398-399 HYPH denotes -
T3910 399-400 NN denotes 3
T3917 400-401 SYM denotes '
T3918 402-403 -LRB- denotes (
T3919 403-410 RB denotes forward
T3920 410-411 -RRB- denotes )
T3921 412-415 CC denotes and
T3922 416-417 CD denotes 5
T3924 417-418 SYM denotes '
T3925 418-419 HYPH denotes -
T3926 419-438 NN denotes AGATCTTGACCACCTGCAG
T3927 438-439 HYPH denotes -
T3923 439-440 NN denotes 3
T3928 440-441 SYM denotes '
T3929 442-443 -LRB- denotes (
T3930 443-450 RB denotes reverse
T3931 450-451 -RRB- denotes )
T3932 452-456 IN denotes with
T3933 457-459 DT denotes an
T3935 460-469 JJ denotes annealing
T3934 470-481 NN denotes temperature
T3936 482-484 IN denotes of
T3937 485-487 CD denotes 59
T3938 487-489 NNS denotes °C
T3939 489-490 -RRB- denotes )
T3940 490-491 . denotes .
T3941 491-562 sentence denotes Hybridization and washes were carried out in standard conditions [16].
T3942 492-505 NN denotes Hybridization
T3944 506-509 CC denotes and
T3945 510-516 NNS denotes washes
T3946 517-521 VBD denotes were
T3943 522-529 VBN denotes carried
T3947 530-533 RP denotes out
T3948 534-536 IN denotes in
T3949 537-545 JJ denotes standard
T3950 546-556 NNS denotes conditions
T3951 557-558 -LRB- denotes [
T3952 558-560 CD denotes 16
T3953 560-561 -RRB- denotes ]
T3954 561-562 . denotes .
R2366 T3835 T3836 compound Southern,Blot
R2367 T3836 T3837 compound Blot,Analysis
R2368 T3839 T3840 amod Total,DNA
R2369 T3840 T3843 nsubjpass DNA,digested
R2370 T3841 T3840 nmod mouse,DNA
R2371 T3842 T3840 amod genomic,DNA
R2372 T3844 T3843 auxpass was,digested
R2373 T3845 T3843 advcl using,digested
R2374 T3846 T3845 dobj EcoRI,using
R2375 T3847 T3846 punct ", ",EcoRI
R2376 T3848 T3846 conj BamHI,EcoRI
R2377 T3849 T3848 punct ", ",BamHI
R2378 T3850 T3848 conj PstI,BamHI
R2379 T3851 T3850 punct ", ",PstI
R2380 T3852 T3850 cc and,PstI
R2381 T3853 T3850 conj XbaI,PstI
R2382 T3854 T3843 punct .,digested
R2383 T3856 T3857 det The,digests
R2384 T3857 T3858 nsubjpass digests,electrophoresed
R2385 T3859 T3858 auxpass were,electrophoresed
R2386 T3860 T3858 prep on,electrophoresed
R2387 T3861 T3862 det a,gel
R2388 T3862 T3860 pobj gel,on
R2389 T3863 T3864 nummod 1,%
R2390 T3864 T3862 compound %,gel
R2391 T3865 T3862 compound agarose,gel
R2392 T3866 T3858 prep at,electrophoresed
R2393 T3867 T3866 pobj 60V,at
R2394 T3868 T3858 advmod overnight,electrophoresed
R2395 T3869 T3858 cc and,electrophoresed
R2396 T3870 T3871 auxpass were,transferred
R2397 T3871 T3858 conj transferred,electrophoresed
R2398 T3872 T3871 prep onto,transferred
R2399 T3873 T3874 det a,membrane
R2400 T3874 T3872 pobj membrane,onto
R2401 T3875 T3874 nmod Hybond,membrane
R2402 T3876 T3875 amod N+,Hybond
R2403 T3877 T3871 prep from,transferred
R2404 T3878 T3879 compound Amersham,Biotech
R2405 T3879 T3877 pobj Biotech,from
R2406 T3880 T3879 compound Pharmacia,Biotech
R2407 T3881 T3882 punct (,Piscataway
R2408 T3882 T3879 parataxis Piscataway,Biotech
R2409 T3883 T3882 punct ", ",Piscataway
R2410 T3884 T3882 npadvmod NJ,Piscataway
R2411 T3885 T3882 punct ),Piscataway
R2412 T3886 T3858 punct .,electrophoresed
R2413 T3888 T3889 det A,fragment
R2414 T3889 T3895 nsubjpass fragment,used
R2415 T3890 T3891 compound 32P,dATP
R2416 T3891 T3893 npadvmod dATP,labeled
R2417 T3892 T3891 punct -,dATP
R2418 T3893 T3889 amod labeled,fragment
R2419 T3894 T3889 compound PCR,fragment
R2420 T3896 T3889 prep of,fragment
R2421 T3897 T3898 det the,region
R2422 T3898 T3896 pobj region,of
R2423 T3899 T3898 compound Mcoln1,region
R2424 T3900 T3898 compound coding,region
R2425 T3901 T3898 acl corresponding,region
R2426 T3902 T3901 prep to,corresponding
R2427 T3903 T3902 pobj exon,to
R2428 T3904 T3903 nummod 2,exon
R2429 T3905 T3895 auxpass was,used
R2430 T3906 T3895 prep as,used
R2431 T3907 T3908 det a,probe
R2432 T3908 T3906 pobj probe,as
R2433 T3909 T3910 punct (,3
R2434 T3910 T3895 parataxis 3,used
R2435 T3911 T3910 nmod primers,3
R2436 T3912 T3910 nummod 5,3
R2437 T3913 T3912 punct ',5
R2438 T3914 T3910 punct -,3
R2439 T3915 T3910 compound CCCCACAGAAGAGGAAGAC,3
R2440 T3916 T3910 punct -,3
R2441 T3917 T3910 punct ',3
R2442 T3918 T3919 punct (,forward
R2443 T3919 T3910 parataxis forward,3
R2444 T3920 T3919 punct ),forward
R2445 T3921 T3910 cc and,3
R2446 T3922 T3923 nummod 5,3
R2447 T3923 T3910 conj 3,3
R2448 T3924 T3922 punct ',5
R2449 T3925 T3923 punct -,3
R2450 T3926 T3923 compound AGATCTTGACCACCTGCAG,3
R2451 T3927 T3923 punct -,3
R2452 T3928 T3923 punct ',3
R2453 T3929 T3930 punct (,reverse
R2454 T3930 T3923 parataxis reverse,3
R2455 T3931 T3930 punct ),reverse
R2456 T3932 T3910 prep with,3
R2457 T3933 T3934 det an,temperature
R2458 T3934 T3932 pobj temperature,with
R2459 T3935 T3934 amod annealing,temperature
R2460 T3936 T3934 prep of,temperature
R2461 T3937 T3938 nummod 59,°C
R2462 T3938 T3936 pobj °C,of
R2463 T3939 T3910 punct ),3
R2464 T3940 T3895 punct .,used
R2465 T3942 T3943 nsubjpass Hybridization,carried
R2466 T3944 T3942 cc and,Hybridization
R2467 T3945 T3942 conj washes,Hybridization
R2468 T3946 T3943 auxpass were,carried
R2469 T3947 T3943 prt out,carried
R2470 T3948 T3943 prep in,carried
R2471 T3949 T3950 amod standard,conditions
R2472 T3950 T3948 pobj conditions,in
R2473 T3951 T3952 punct [,16
R2474 T3952 T3943 parataxis 16,carried
R2475 T3953 T3952 punct ],16
R2476 T3954 T3943 punct .,carried