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PMC:88885 / 14545-14984 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3694 0-4 NN denotes BALB
T3696 4-5 HYPH denotes /
T3695 5-6 NN denotes c
T3697 7-12 NN denotes mouse
T3698 13-18 NN denotes brain
T3700 19-24 JJ denotes total
T3699 25-28 NN denotes RNA
T3702 29-32 VBD denotes was
T3701 33-42 VBN denotes purchased
T3703 43-47 IN denotes from
T3704 48-56 NNP denotes Clontech
T3705 57-69 NNP denotes Laboratories
T3706 70-71 -LRB- denotes (
T3708 71-75 NNP denotes Palo
T3707 76-80 NNP denotes Alto
T3709 80-82 , denotes ,
T3710 82-84 NNP denotes CA
T3711 84-85 -RRB- denotes )
T3712 86-89 CC denotes and
T3713 90-94 VBN denotes made
T3714 95-97 IN denotes to
T3715 98-99 DT denotes a
T3717 100-101 CD denotes 1
T3718 102-104 NN denotes μg
T3719 105-106 SYM denotes /
T3720 107-109 NN denotes ml
T3716 110-123 NN denotes concentration
T3721 123-124 . denotes .
T3722 124-225 sentence denotes This RNA was used as a template to create cDNA via RT-PCR using random hexamers and oligo dT primer.
T3723 125-129 DT denotes This
T3724 130-133 NN denotes RNA
T3726 134-137 VBD denotes was
T3725 138-142 VBN denotes used
T3727 143-145 IN denotes as
T3728 146-147 DT denotes a
T3729 148-156 NN denotes template
T3730 157-159 TO denotes to
T3731 160-166 VB denotes create
T3732 167-171 NN denotes cDNA
T3733 172-175 IN denotes via
T3734 176-178 NN denotes RT
T3736 178-179 HYPH denotes -
T3735 179-182 NN denotes PCR
T3737 183-188 VBG denotes using
T3738 189-195 JJ denotes random
T3739 196-204 NNS denotes hexamers
T3740 205-208 CC denotes and
T3741 209-214 NN denotes oligo
T3743 215-217 NN denotes dT
T3742 218-224 NN denotes primer
T3744 224-225 . denotes .
T3745 225-439 sentence denotes The RT product was used to confirm the alternatively spliced form using primers: 5'-CATCTACCTGGGCTATTGC-3' (forward) and 5'-GCTCTCAGGTGGTGGACAC-3' (reverse) in a PCR reaction with an annealing temperature of 61°C.
T3746 226-229 DT denotes The
T3748 230-232 NN denotes RT
T3747 233-240 NN denotes product
T3750 241-244 VBD denotes was
T3749 245-249 VBN denotes used
T3751 250-252 TO denotes to
T3752 253-260 VB denotes confirm
T3753 261-264 DT denotes the
T3755 265-278 RB denotes alternatively
T3756 279-286 VBN denotes spliced
T3754 287-291 NN denotes form
T3757 292-297 VBG denotes using
T3758 298-305 NNS denotes primers
T3759 305-307 : denotes :
T3760 307-308 CD denotes 5
T3762 308-309 SYM denotes '
T3763 309-310 HYPH denotes -
T3764 310-329 NN denotes CATCTACCTGGGCTATTGC
T3765 329-330 HYPH denotes -
T3761 330-331 NN denotes 3
T3766 331-332 SYM denotes '
T3767 333-334 -LRB- denotes (
T3768 334-341 RB denotes forward
T3769 341-342 -RRB- denotes )
T3770 343-346 CC denotes and
T3771 347-348 CD denotes 5
T3773 348-349 SYM denotes '
T3774 349-350 HYPH denotes -
T3775 350-369 NN denotes GCTCTCAGGTGGTGGACAC
T3776 369-370 HYPH denotes -
T3772 370-371 NN denotes 3
T3777 371-372 SYM denotes '
T3778 373-374 -LRB- denotes (
T3779 374-381 RB denotes reverse
T3780 381-382 -RRB- denotes )
T3781 383-385 IN denotes in
T3782 386-387 DT denotes a
T3784 388-391 NN denotes PCR
T3783 392-400 NN denotes reaction
T3785 401-405 IN denotes with
T3786 406-408 DT denotes an
T3788 409-418 JJ denotes annealing
T3787 419-430 NN denotes temperature
T3789 431-433 IN denotes of
T3790 434-436 CD denotes 61
T3791 436-438 NNS denotes °C
T3792 438-439 . denotes .
R2271 T3694 T3695 nmod BALB,c
R2272 T3695 T3697 nmod c,mouse
R2273 T3696 T3695 punct /,c
R2274 T3697 T3698 nmod mouse,brain
R2275 T3698 T3699 nmod brain,RNA
R2276 T3699 T3701 nsubjpass RNA,purchased
R2277 T3700 T3699 amod total,RNA
R2278 T3702 T3701 auxpass was,purchased
R2279 T3703 T3701 prep from,purchased
R2280 T3704 T3705 compound Clontech,Laboratories
R2281 T3705 T3703 pobj Laboratories,from
R2282 T3706 T3707 punct (,Alto
R2283 T3707 T3705 parataxis Alto,Laboratories
R2284 T3708 T3707 compound Palo,Alto
R2285 T3709 T3707 punct ", ",Alto
R2286 T3710 T3707 npadvmod CA,Alto
R2287 T3711 T3707 punct ),Alto
R2288 T3712 T3701 cc and,purchased
R2289 T3713 T3701 conj made,purchased
R2290 T3714 T3713 prep to,made
R2291 T3715 T3716 det a,concentration
R2292 T3716 T3714 pobj concentration,to
R2293 T3717 T3718 nummod 1,μg
R2294 T3718 T3716 nmod μg,concentration
R2295 T3719 T3720 punct /,ml
R2296 T3720 T3718 prep ml,μg
R2297 T3721 T3701 punct .,purchased
R2298 T3723 T3724 det This,RNA
R2299 T3724 T3725 nsubjpass RNA,used
R2300 T3726 T3725 auxpass was,used
R2301 T3727 T3725 prep as,used
R2302 T3728 T3729 det a,template
R2303 T3729 T3727 pobj template,as
R2304 T3730 T3731 aux to,create
R2305 T3731 T3725 advcl create,used
R2306 T3732 T3731 dobj cDNA,create
R2307 T3733 T3731 prep via,create
R2308 T3734 T3735 compound RT,PCR
R2309 T3735 T3733 pobj PCR,via
R2310 T3736 T3735 punct -,PCR
R2311 T3737 T3731 advcl using,create
R2312 T3738 T3739 amod random,hexamers
R2313 T3739 T3737 dobj hexamers,using
R2314 T3740 T3739 cc and,hexamers
R2315 T3741 T3742 compound oligo,primer
R2316 T3742 T3739 conj primer,hexamers
R2317 T3743 T3742 compound dT,primer
R2318 T3744 T3725 punct .,used
R2319 T3746 T3747 det The,product
R2320 T3747 T3749 nsubjpass product,used
R2321 T3748 T3747 compound RT,product
R2322 T3750 T3749 auxpass was,used
R2323 T3751 T3752 aux to,confirm
R2324 T3752 T3749 advcl confirm,used
R2325 T3753 T3754 det the,form
R2326 T3754 T3752 dobj form,confirm
R2327 T3755 T3756 advmod alternatively,spliced
R2328 T3756 T3754 amod spliced,form
R2329 T3757 T3752 advcl using,confirm
R2330 T3758 T3757 dobj primers,using
R2331 T3759 T3758 punct : ,primers
R2332 T3760 T3761 nummod 5,3
R2333 T3761 T3758 appos 3,primers
R2334 T3762 T3760 punct ',5
R2335 T3763 T3761 punct -,3
R2336 T3764 T3761 compound CATCTACCTGGGCTATTGC,3
R2337 T3765 T3761 punct -,3
R2338 T3766 T3761 punct ',3
R2339 T3767 T3768 punct (,forward
R2340 T3768 T3761 parataxis forward,3
R2341 T3769 T3768 punct ),forward
R2342 T3770 T3761 cc and,3
R2343 T3771 T3772 nummod 5,3
R2344 T3772 T3761 conj 3,3
R2345 T3773 T3771 punct ',5
R2346 T3774 T3772 punct -,3
R2347 T3775 T3772 compound GCTCTCAGGTGGTGGACAC,3
R2348 T3776 T3772 punct -,3
R2349 T3777 T3772 punct ',3
R2350 T3778 T3779 punct (,reverse
R2351 T3779 T3772 parataxis reverse,3
R2352 T3780 T3779 punct ),reverse
R2353 T3781 T3757 prep in,using
R2354 T3782 T3783 det a,reaction
R2355 T3783 T3781 pobj reaction,in
R2356 T3784 T3783 compound PCR,reaction
R2357 T3785 T3757 prep with,using
R2358 T3786 T3787 det an,temperature
R2359 T3787 T3785 pobj temperature,with
R2360 T3788 T3787 amod annealing,temperature
R2361 T3789 T3787 prep of,temperature
R2362 T3790 T3791 nummod 61,°C
R2363 T3791 T3789 pobj °C,of
R2364 T3792 T3749 punct .,used