| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T3689 |
0-2 |
NN |
denotes |
RT |
| T3691 |
2-3 |
HYPH |
denotes |
- |
| T3690 |
3-6 |
NN |
denotes |
PCR |
| T3692 |
7-15 |
NN |
denotes |
Reaction |
| T3693 |
15-140 |
sentence |
denotes |
BALB/c mouse brain total RNA was purchased from Clontech Laboratories (Palo Alto, CA) and made to a 1 μg / ml concentration. |
| T3694 |
16-20 |
NN |
denotes |
BALB |
| T3696 |
20-21 |
HYPH |
denotes |
/ |
| T3695 |
21-22 |
NN |
denotes |
c |
| T3697 |
23-28 |
NN |
denotes |
mouse |
| T3698 |
29-34 |
NN |
denotes |
brain |
| T3700 |
35-40 |
JJ |
denotes |
total |
| T3699 |
41-44 |
NN |
denotes |
RNA |
| T3702 |
45-48 |
VBD |
denotes |
was |
| T3701 |
49-58 |
VBN |
denotes |
purchased |
| T3703 |
59-63 |
IN |
denotes |
from |
| T3704 |
64-72 |
NNP |
denotes |
Clontech |
| T3705 |
73-85 |
NNP |
denotes |
Laboratories |
| T3706 |
86-87 |
-LRB- |
denotes |
( |
| T3708 |
87-91 |
NNP |
denotes |
Palo |
| T3707 |
92-96 |
NNP |
denotes |
Alto |
| T3709 |
96-98 |
, |
denotes |
, |
| T3710 |
98-100 |
NNP |
denotes |
CA |
| T3711 |
100-101 |
-RRB- |
denotes |
) |
| T3712 |
102-105 |
CC |
denotes |
and |
| T3713 |
106-110 |
VBN |
denotes |
made |
| T3714 |
111-113 |
IN |
denotes |
to |
| T3715 |
114-115 |
DT |
denotes |
a |
| T3717 |
116-117 |
CD |
denotes |
1 |
| T3718 |
118-120 |
NN |
denotes |
μg |
| T3719 |
121-122 |
SYM |
denotes |
/ |
| T3720 |
123-125 |
NN |
denotes |
ml |
| T3716 |
126-139 |
NN |
denotes |
concentration |
| T3721 |
139-140 |
. |
denotes |
. |
| T3722 |
140-241 |
sentence |
denotes |
This RNA was used as a template to create cDNA via RT-PCR using random hexamers and oligo dT primer. |
| T3723 |
141-145 |
DT |
denotes |
This |
| T3724 |
146-149 |
NN |
denotes |
RNA |
| T3726 |
150-153 |
VBD |
denotes |
was |
| T3725 |
154-158 |
VBN |
denotes |
used |
| T3727 |
159-161 |
IN |
denotes |
as |
| T3728 |
162-163 |
DT |
denotes |
a |
| T3729 |
164-172 |
NN |
denotes |
template |
| T3730 |
173-175 |
TO |
denotes |
to |
| T3731 |
176-182 |
VB |
denotes |
create |
| T3732 |
183-187 |
NN |
denotes |
cDNA |
| T3733 |
188-191 |
IN |
denotes |
via |
| T3734 |
192-194 |
NN |
denotes |
RT |
| T3736 |
194-195 |
HYPH |
denotes |
- |
| T3735 |
195-198 |
NN |
denotes |
PCR |
| T3737 |
199-204 |
VBG |
denotes |
using |
| T3738 |
205-211 |
JJ |
denotes |
random |
| T3739 |
212-220 |
NNS |
denotes |
hexamers |
| T3740 |
221-224 |
CC |
denotes |
and |
| T3741 |
225-230 |
NN |
denotes |
oligo |
| T3743 |
231-233 |
NN |
denotes |
dT |
| T3742 |
234-240 |
NN |
denotes |
primer |
| T3744 |
240-241 |
. |
denotes |
. |
| T3745 |
241-455 |
sentence |
denotes |
The RT product was used to confirm the alternatively spliced form using primers: 5'-CATCTACCTGGGCTATTGC-3' (forward) and 5'-GCTCTCAGGTGGTGGACAC-3' (reverse) in a PCR reaction with an annealing temperature of 61°C. |
| T3746 |
242-245 |
DT |
denotes |
The |
| T3748 |
246-248 |
NN |
denotes |
RT |
| T3747 |
249-256 |
NN |
denotes |
product |
| T3750 |
257-260 |
VBD |
denotes |
was |
| T3749 |
261-265 |
VBN |
denotes |
used |
| T3751 |
266-268 |
TO |
denotes |
to |
| T3752 |
269-276 |
VB |
denotes |
confirm |
| T3753 |
277-280 |
DT |
denotes |
the |
| T3755 |
281-294 |
RB |
denotes |
alternatively |
| T3756 |
295-302 |
VBN |
denotes |
spliced |
| T3754 |
303-307 |
NN |
denotes |
form |
| T3757 |
308-313 |
VBG |
denotes |
using |
| T3758 |
314-321 |
NNS |
denotes |
primers |
| T3759 |
321-323 |
: |
denotes |
: |
| T3760 |
323-324 |
CD |
denotes |
5 |
| T3762 |
324-325 |
SYM |
denotes |
' |
| T3763 |
325-326 |
HYPH |
denotes |
- |
| T3764 |
326-345 |
NN |
denotes |
CATCTACCTGGGCTATTGC |
| T3765 |
345-346 |
HYPH |
denotes |
- |
| T3761 |
346-347 |
NN |
denotes |
3 |
| T3766 |
347-348 |
SYM |
denotes |
' |
| T3767 |
349-350 |
-LRB- |
denotes |
( |
| T3768 |
350-357 |
RB |
denotes |
forward |
| T3769 |
357-358 |
-RRB- |
denotes |
) |
| T3770 |
359-362 |
CC |
denotes |
and |
| T3771 |
363-364 |
CD |
denotes |
5 |
| T3773 |
364-365 |
SYM |
denotes |
' |
| T3774 |
365-366 |
HYPH |
denotes |
- |
| T3775 |
366-385 |
NN |
denotes |
GCTCTCAGGTGGTGGACAC |
| T3776 |
385-386 |
HYPH |
denotes |
- |
| T3772 |
386-387 |
NN |
denotes |
3 |
| T3777 |
387-388 |
SYM |
denotes |
' |
| T3778 |
389-390 |
-LRB- |
denotes |
( |
| T3779 |
390-397 |
RB |
denotes |
reverse |
| T3780 |
397-398 |
-RRB- |
denotes |
) |
| T3781 |
399-401 |
IN |
denotes |
in |
| T3782 |
402-403 |
DT |
denotes |
a |
| T3784 |
404-407 |
NN |
denotes |
PCR |
| T3783 |
408-416 |
NN |
denotes |
reaction |
| T3785 |
417-421 |
IN |
denotes |
with |
| T3786 |
422-424 |
DT |
denotes |
an |
| T3788 |
425-434 |
JJ |
denotes |
annealing |
| T3787 |
435-446 |
NN |
denotes |
temperature |
| T3789 |
447-449 |
IN |
denotes |
of |
| T3790 |
450-452 |
CD |
denotes |
61 |
| T3791 |
452-454 |
NNS |
denotes |
°C |
| T3792 |
454-455 |
. |
denotes |
. |
| R2268 |
T3689 |
T3690 |
compound |
RT,PCR |
| R2269 |
T3690 |
T3692 |
compound |
PCR,Reaction |
| R2270 |
T3691 |
T3690 |
punct |
-,PCR |
| R2271 |
T3694 |
T3695 |
nmod |
BALB,c |
| R2272 |
T3695 |
T3697 |
nmod |
c,mouse |
| R2273 |
T3696 |
T3695 |
punct |
/,c |
| R2274 |
T3697 |
T3698 |
nmod |
mouse,brain |
| R2275 |
T3698 |
T3699 |
nmod |
brain,RNA |
| R2276 |
T3699 |
T3701 |
nsubjpass |
RNA,purchased |
| R2277 |
T3700 |
T3699 |
amod |
total,RNA |
| R2278 |
T3702 |
T3701 |
auxpass |
was,purchased |
| R2279 |
T3703 |
T3701 |
prep |
from,purchased |
| R2280 |
T3704 |
T3705 |
compound |
Clontech,Laboratories |
| R2281 |
T3705 |
T3703 |
pobj |
Laboratories,from |
| R2282 |
T3706 |
T3707 |
punct |
(,Alto |
| R2283 |
T3707 |
T3705 |
parataxis |
Alto,Laboratories |
| R2284 |
T3708 |
T3707 |
compound |
Palo,Alto |
| R2285 |
T3709 |
T3707 |
punct |
", ",Alto |
| R2286 |
T3710 |
T3707 |
npadvmod |
CA,Alto |
| R2287 |
T3711 |
T3707 |
punct |
),Alto |
| R2288 |
T3712 |
T3701 |
cc |
and,purchased |
| R2289 |
T3713 |
T3701 |
conj |
made,purchased |
| R2290 |
T3714 |
T3713 |
prep |
to,made |
| R2291 |
T3715 |
T3716 |
det |
a,concentration |
| R2292 |
T3716 |
T3714 |
pobj |
concentration,to |
| R2293 |
T3717 |
T3718 |
nummod |
1,μg |
| R2294 |
T3718 |
T3716 |
nmod |
μg,concentration |
| R2295 |
T3719 |
T3720 |
punct |
/,ml |
| R2296 |
T3720 |
T3718 |
prep |
ml,μg |
| R2297 |
T3721 |
T3701 |
punct |
.,purchased |
| R2298 |
T3723 |
T3724 |
det |
This,RNA |
| R2299 |
T3724 |
T3725 |
nsubjpass |
RNA,used |
| R2300 |
T3726 |
T3725 |
auxpass |
was,used |
| R2301 |
T3727 |
T3725 |
prep |
as,used |
| R2302 |
T3728 |
T3729 |
det |
a,template |
| R2303 |
T3729 |
T3727 |
pobj |
template,as |
| R2304 |
T3730 |
T3731 |
aux |
to,create |
| R2305 |
T3731 |
T3725 |
advcl |
create,used |
| R2306 |
T3732 |
T3731 |
dobj |
cDNA,create |
| R2307 |
T3733 |
T3731 |
prep |
via,create |
| R2308 |
T3734 |
T3735 |
compound |
RT,PCR |
| R2309 |
T3735 |
T3733 |
pobj |
PCR,via |
| R2310 |
T3736 |
T3735 |
punct |
-,PCR |
| R2311 |
T3737 |
T3731 |
advcl |
using,create |
| R2312 |
T3738 |
T3739 |
amod |
random,hexamers |
| R2313 |
T3739 |
T3737 |
dobj |
hexamers,using |
| R2314 |
T3740 |
T3739 |
cc |
and,hexamers |
| R2315 |
T3741 |
T3742 |
compound |
oligo,primer |
| R2316 |
T3742 |
T3739 |
conj |
primer,hexamers |
| R2317 |
T3743 |
T3742 |
compound |
dT,primer |
| R2318 |
T3744 |
T3725 |
punct |
.,used |
| R2319 |
T3746 |
T3747 |
det |
The,product |
| R2320 |
T3747 |
T3749 |
nsubjpass |
product,used |
| R2321 |
T3748 |
T3747 |
compound |
RT,product |
| R2322 |
T3750 |
T3749 |
auxpass |
was,used |
| R2323 |
T3751 |
T3752 |
aux |
to,confirm |
| R2324 |
T3752 |
T3749 |
advcl |
confirm,used |
| R2325 |
T3753 |
T3754 |
det |
the,form |
| R2326 |
T3754 |
T3752 |
dobj |
form,confirm |
| R2327 |
T3755 |
T3756 |
advmod |
alternatively,spliced |
| R2328 |
T3756 |
T3754 |
amod |
spliced,form |
| R2329 |
T3757 |
T3752 |
advcl |
using,confirm |
| R2330 |
T3758 |
T3757 |
dobj |
primers,using |
| R2331 |
T3759 |
T3758 |
punct |
: ,primers |
| R2332 |
T3760 |
T3761 |
nummod |
5,3 |
| R2333 |
T3761 |
T3758 |
appos |
3,primers |
| R2334 |
T3762 |
T3760 |
punct |
',5 |
| R2335 |
T3763 |
T3761 |
punct |
-,3 |
| R2336 |
T3764 |
T3761 |
compound |
CATCTACCTGGGCTATTGC,3 |
| R2337 |
T3765 |
T3761 |
punct |
-,3 |
| R2338 |
T3766 |
T3761 |
punct |
',3 |
| R2339 |
T3767 |
T3768 |
punct |
(,forward |
| R2340 |
T3768 |
T3761 |
parataxis |
forward,3 |
| R2341 |
T3769 |
T3768 |
punct |
),forward |
| R2342 |
T3770 |
T3761 |
cc |
and,3 |
| R2343 |
T3771 |
T3772 |
nummod |
5,3 |
| R2344 |
T3772 |
T3761 |
conj |
3,3 |
| R2345 |
T3773 |
T3771 |
punct |
',5 |
| R2346 |
T3774 |
T3772 |
punct |
-,3 |
| R2347 |
T3775 |
T3772 |
compound |
GCTCTCAGGTGGTGGACAC,3 |
| R2348 |
T3776 |
T3772 |
punct |
-,3 |
| R2349 |
T3777 |
T3772 |
punct |
',3 |
| R2350 |
T3778 |
T3779 |
punct |
(,reverse |
| R2351 |
T3779 |
T3772 |
parataxis |
reverse,3 |
| R2352 |
T3780 |
T3779 |
punct |
),reverse |
| R2353 |
T3781 |
T3757 |
prep |
in,using |
| R2354 |
T3782 |
T3783 |
det |
a,reaction |
| R2355 |
T3783 |
T3781 |
pobj |
reaction,in |
| R2356 |
T3784 |
T3783 |
compound |
PCR,reaction |
| R2357 |
T3785 |
T3757 |
prep |
with,using |
| R2358 |
T3786 |
T3787 |
det |
an,temperature |
| R2359 |
T3787 |
T3785 |
pobj |
temperature,with |
| R2360 |
T3788 |
T3787 |
amod |
annealing,temperature |
| R2361 |
T3789 |
T3787 |
prep |
of,temperature |
| R2362 |
T3790 |
T3791 |
nummod |
61,°C |
| R2363 |
T3791 |
T3789 |
pobj |
°C,of |
| R2364 |
T3792 |
T3749 |
punct |
.,used |