PMC:88885 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T115	0-7	NN	denotes	Cloning
T1035	0-7	NN	denotes	Cloning
T117	0-116	sentence	denotes	Cloning and characterization of the mouse Mcoln1 gene reveals an alternatively spliced transcript not seen in humans
T118	8-11	CC	denotes	and
T1036	8-11	CC	denotes	and
T119	12-28	NN	denotes	characterization
T120	29-31	IN	denotes	of
T121	32-35	DT	denotes	the
T123	36-41	NN	denotes	mouse
T124	42-48	NN	denotes	Mcoln1
T122	49-53	NN	denotes	gene
T116	54-61	VBZ	denotes	reveals
T125	62-64	DT	denotes	an
T127	65-78	RB	denotes	alternatively
T128	79-86	VBN	denotes	spliced
T126	87-97	NN	denotes	transcript
T129	98-101	RB	denotes	not
T130	102-106	VBN	denotes	seen
T131	107-109	IN	denotes	in
T132	110-116	NNS	denotes	humans
T133	116-117	sentence	denotes
T134	127-137	sentence	denotes	Background
T135	127-137	NN	denotes	Background
T136	137-288	sentence	denotes	Mucolipidosis type IV (MLIV) is an autosomal recessive lysosomal storage disorder characterized by severe neurologic and ophthalmologic abnormalities.
T137	138-151	NN	denotes	Mucolipidosis
T138	152-156	NN	denotes	type
T140	157-159	CD	denotes	IV
T141	160-161	-LRB-	denotes	(
T142	161-165	NN	denotes	MLIV
T143	165-166	-RRB-	denotes	)
T139	167-169	VBZ	denotes	is
T144	170-172	DT	denotes	an
T146	173-182	JJ	denotes	autosomal
T147	183-192	JJ	denotes	recessive
T148	193-202	JJ	denotes	lysosomal
T149	203-210	NN	denotes	storage
T145	211-219	NN	denotes	disorder
T150	220-233	VBN	denotes	characterized
T151	234-236	IN	denotes	by
T152	237-243	JJ	denotes	severe
T154	244-254	JJ	denotes	neurologic
T155	255-258	CC	denotes	and
T156	259-273	JJ	denotes	ophthalmologic
T153	274-287	NNS	denotes	abnormalities
T157	287-288	.	denotes	.
T158	288-426	sentence	denotes	Recently the MLIV gene, MCOLN1, has been identified as a new member of the transient receptor potential (TRP) cation channel superfamily.
T159	289-297	RB	denotes	Recently
T161	298-301	DT	denotes	the
T163	302-306	NN	denotes	MLIV
T162	307-311	NN	denotes	gene
T164	311-313	,	denotes	,
T165	313-319	NN	denotes	MCOLN1
T166	319-321	,	denotes	,
T167	321-324	VBZ	denotes	has
T168	325-329	VBN	denotes	been
T160	330-340	VBN	denotes	identified
T169	341-343	IN	denotes	as
T170	344-345	DT	denotes	a
T172	346-349	JJ	denotes	new
T171	350-356	NN	denotes	member
T173	357-359	IN	denotes	of
T174	360-363	DT	denotes	the
T176	364-373	JJ	denotes	transient
T178	374-382	NN	denotes	receptor
T177	383-392	NN	denotes	potential
T179	393-394	-LRB-	denotes	(
T180	394-397	NN	denotes	TRP
T181	397-398	-RRB-	denotes	)
T182	399-405	NN	denotes	cation
T183	406-413	NN	denotes	channel
T175	414-425	NN	denotes	superfamily
T184	425-426	.	denotes	.
T185	426-568	sentence	denotes	Here we report the cloning and characterization of the mouse homologue, Mcoln1, and report a novel splice variant that is not seen in humans.
T186	427-431	RB	denotes	Here
T188	432-434	PRP	denotes	we
T187	435-441	VBP	denotes	report
T189	442-445	DT	denotes	the
T190	446-453	NN	denotes	cloning
T191	454-457	CC	denotes	and
T192	458-474	NN	denotes	characterization
T193	475-477	IN	denotes	of
T194	478-481	DT	denotes	the
T196	482-487	NN	denotes	mouse
T195	488-497	NN	denotes	homologue
T197	497-499	,	denotes	,
T198	499-505	NN	denotes	Mcoln1
T199	505-507	,	denotes	,
T200	507-510	CC	denotes	and
T201	511-517	VBP	denotes	report
T202	518-519	DT	denotes	a
T204	520-525	JJ	denotes	novel
T205	526-532	NN	denotes	splice
T203	533-540	NN	denotes	variant
T206	541-545	WDT	denotes	that
T208	546-548	VBZ	denotes	is
T209	549-552	RB	denotes	not
T207	553-557	VBN	denotes	seen
T210	558-560	IN	denotes	in
T211	561-567	NNS	denotes	humans
T212	567-568	.	denotes	.
T213	568-577	sentence	denotes	Results
T214	570-577	NNS	denotes	Results
T215	577-637	sentence	denotes	The human and mouse genes display a high degree of synteny.
T216	578-581	DT	denotes	The
T218	582-587	JJ	denotes	human
T220	588-591	CC	denotes	and
T219	592-597	NN	denotes	mouse
T217	598-603	NNS	denotes	genes
T221	604-611	VBP	denotes	display
T222	612-613	DT	denotes	a
T224	614-618	JJ	denotes	high
T223	619-625	NN	denotes	degree
T225	626-628	IN	denotes	of
T226	629-636	NN	denotes	synteny
T227	636-637	.	denotes	.
T228	637-704	sentence	denotes	Mcoln1 shows 91% amino acid and 86% nucleotide identity to MCOLN1.
T229	638-644	NN	denotes	Mcoln1
T230	645-650	VBZ	denotes	shows
T231	651-653	CD	denotes	91
T232	653-654	NN	denotes	%
T234	655-660	NN	denotes	amino
T233	661-665	NN	denotes	acid
T236	666-669	CC	denotes	and
T237	670-672	CD	denotes	86
T238	672-673	NN	denotes	%
T239	674-684	NN	denotes	nucleotide
T235	685-693	NN	denotes	identity
T240	694-696	IN	denotes	to
T241	697-703	NN	denotes	MCOLN1
T242	703-704	.	denotes	.
T243	704-895	sentence	denotes	Also, Mcoln1 maps to chromosome 8 and contains an open reading frame of 580 amino acids, with a transcript length of approximately 2 kb encoded by 14 exons, similar to its human counterpart.
T244	705-709	RB	denotes	Also
T246	709-711	,	denotes	,
T247	711-717	NN	denotes	Mcoln1
T245	718-722	VBZ	denotes	maps
T248	723-725	IN	denotes	to
T249	726-736	NN	denotes	chromosome
T250	737-738	CD	denotes	8
T251	739-742	CC	denotes	and
T252	743-751	VBZ	denotes	contains
T253	752-754	DT	denotes	an
T255	755-759	JJ	denotes	open
T256	760-767	NN	denotes	reading
T254	768-773	NN	denotes	frame
T257	774-776	IN	denotes	of
T258	777-780	CD	denotes	580
T260	781-786	NN	denotes	amino
T259	787-792	NNS	denotes	acids
T261	792-794	,	denotes	,
T262	794-798	IN	denotes	with
T263	799-800	DT	denotes	a
T265	801-811	NN	denotes	transcript
T264	812-818	NN	denotes	length
T266	819-821	IN	denotes	of
T267	822-835	RB	denotes	approximately
T268	836-837	CD	denotes	2
T269	838-840	NNS	denotes	kb
T270	841-848	VBN	denotes	encoded
T271	849-851	IN	denotes	by
T272	852-854	CD	denotes	14
T273	855-860	NNS	denotes	exons
T274	860-862	,	denotes	,
T275	862-869	JJ	denotes	similar
T276	870-872	IN	denotes	to
T277	873-876	PRP$	denotes	its
T279	877-882	JJ	denotes	human
T278	883-894	NN	denotes	counterpart
T280	894-895	.	denotes	.
T281	895-1030	sentence	denotes	The transcript that results from murine specific alternative splicing encodes a 611 amino acid protein that differs at the c-terminus.
T282	896-899	DT	denotes	The
T283	900-910	NN	denotes	transcript
T285	911-915	WDT	denotes	that
T286	916-923	VBZ	denotes	results
T287	924-928	IN	denotes	from
T288	929-935	NN	denotes	murine
T289	936-944	JJ	denotes	specific
T291	945-956	JJ	denotes	alternative
T290	957-965	NN	denotes	splicing
T284	966-973	VBZ	denotes	encodes
T292	974-975	DT	denotes	a
T294	976-979	CD	denotes	611
T296	980-985	NN	denotes	amino
T295	986-990	NN	denotes	acid
T293	991-998	NN	denotes	protein
T297	999-1003	WDT	denotes	that
T298	1004-1011	VBZ	denotes	differs
T299	1012-1014	IN	denotes	at
T300	1015-1018	DT	denotes	the
T302	1019-1020	NN	denotes	c
T303	1020-1021	HYPH	denotes	-
T301	1021-1029	NN	denotes	terminus
T304	1029-1030	.	denotes	.
T305	1030-1043	sentence	denotes	Conclusions
T306	1032-1043	NNS	denotes	Conclusions
T307	1043-1140	sentence	denotes	Mcoln1 is highly similar to MCOLN1, especially in the transmembrane domains and ion pore region.
T308	1044-1050	NN	denotes	Mcoln1
T309	1051-1053	VBZ	denotes	is
T310	1054-1060	RB	denotes	highly
T311	1061-1068	JJ	denotes	similar
T312	1069-1071	IN	denotes	to
T313	1072-1078	NN	denotes	MCOLN1
T314	1078-1080	,	denotes	,
T315	1080-1090	RB	denotes	especially
T316	1091-1093	IN	denotes	in
T317	1094-1097	DT	denotes	the
T319	1098-1111	NN	denotes	transmembrane
T318	1112-1119	NNS	denotes	domains
T320	1120-1123	CC	denotes	and
T321	1124-1127	NN	denotes	ion
T322	1128-1132	NN	denotes	pore
T323	1133-1139	NN	denotes	region
T324	1139-1140	.	denotes	.
T325	1140-1290	sentence	denotes	Also, the late endosomal/lysosomal targeting signal is conserved, supporting the hypothesis that the protein is localized to these vesicle membranes.
T326	1141-1145	RB	denotes	Also
T328	1145-1147	,	denotes	,
T329	1147-1150	DT	denotes	the
T331	1151-1155	JJ	denotes	late
T332	1156-1165	JJ	denotes	endosomal
T334	1165-1166	HYPH	denotes	/
T333	1166-1175	JJ	denotes	lysosomal
T335	1176-1185	NN	denotes	targeting
T330	1186-1192	NN	denotes	signal
T336	1193-1195	VBZ	denotes	is
T327	1196-1205	VBN	denotes	conserved
T337	1205-1207	,	denotes	,
T338	1207-1217	VBG	denotes	supporting
T339	1218-1221	DT	denotes	the
T340	1222-1232	NN	denotes	hypothesis
T341	1233-1237	IN	denotes	that
T343	1238-1241	DT	denotes	the
T344	1242-1249	NN	denotes	protein
T345	1250-1252	VBZ	denotes	is
T342	1253-1262	VBN	denotes	localized
T346	1263-1265	IN	denotes	to
T347	1266-1271	DT	denotes	these
T349	1272-1279	NN	denotes	vesicle
T348	1280-1289	NNS	denotes	membranes
T350	1289-1290	.	denotes	.
T351	1290-1371	sentence	denotes	To date, there are very few reports describing species-specific splice variants.
T352	1291-1293	IN	denotes	To
T354	1294-1298	NN	denotes	date
T355	1298-1300	,	denotes	,
T356	1300-1305	EX	denotes	there
T353	1306-1309	VBP	denotes	are
T357	1310-1314	RB	denotes	very
T358	1315-1318	JJ	denotes	few
T359	1319-1326	NNS	denotes	reports
T360	1327-1337	VBG	denotes	describing
T361	1338-1345	NN	denotes	species
T363	1345-1346	HYPH	denotes	-
T362	1346-1354	JJ	denotes	specific
T365	1355-1361	NN	denotes	splice
T364	1362-1370	NNS	denotes	variants
T366	1370-1371	.	denotes	.
T367	1371-1606	sentence	denotes	While identification of Mcoln1 is crucial to the development of mouse models for MLIV, the fact that there are two transcripts in mice suggests an additional or alternate function of the gene that may complicate phenotypic assessment.
T368	1372-1377	IN	denotes	While
T370	1378-1392	NN	denotes	identification
T371	1393-1395	IN	denotes	of
T372	1396-1402	NN	denotes	Mcoln1
T369	1403-1405	VBZ	denotes	is
T374	1406-1413	JJ	denotes	crucial
T375	1414-1416	IN	denotes	to
T376	1417-1420	DT	denotes	the
T377	1421-1432	NN	denotes	development
T378	1433-1435	IN	denotes	of
T379	1436-1441	NN	denotes	mouse
T380	1442-1448	NNS	denotes	models
T381	1449-1452	IN	denotes	for
T382	1453-1457	NN	denotes	MLIV
T383	1457-1459	,	denotes	,
T384	1459-1462	DT	denotes	the
T385	1463-1467	NN	denotes	fact
T386	1468-1472	IN	denotes	that
T388	1473-1478	EX	denotes	there
T387	1479-1482	VBP	denotes	are
T389	1483-1486	CD	denotes	two
T390	1487-1498	NNS	denotes	transcripts
T391	1499-1501	IN	denotes	in
T392	1502-1506	NNS	denotes	mice
T373	1507-1515	VBZ	denotes	suggests
T393	1516-1518	DT	denotes	an
T395	1519-1529	JJ	denotes	additional
T396	1530-1532	CC	denotes	or
T397	1533-1542	JJ	denotes	alternate
T394	1543-1551	NN	denotes	function
T398	1552-1554	IN	denotes	of
T399	1555-1558	DT	denotes	the
T400	1559-1563	NN	denotes	gene
T401	1564-1568	WDT	denotes	that
T403	1569-1572	MD	denotes	may
T402	1573-1583	VB	denotes	complicate
T404	1584-1594	JJ	denotes	phenotypic
T405	1595-1605	NN	denotes	assessment
T406	1605-1606	.	denotes	.
T560	1619-1632	NN	denotes	Mucolipidosis
T561	1633-1637	NN	denotes	type
T563	1638-1640	CD	denotes	IV
T564	1641-1642	-LRB-	denotes	(
T565	1642-1646	NN	denotes	MLIV
T566	1646-1647	:	denotes	;
T567	1648-1651	NN	denotes	MIM
T568	1652-1658	CD	denotes	252650
T569	1658-1659	-RRB-	denotes	)
T562	1660-1662	VBZ	denotes	is
T570	1663-1665	DT	denotes	an
T572	1666-1675	JJ	denotes	autosomal
T573	1676-1685	JJ	denotes	recessive
T574	1686-1695	JJ	denotes	lysosomal
T575	1696-1703	NN	denotes	storage
T571	1704-1712	NN	denotes	disorder
T576	1713-1717	WDT	denotes	that
T578	1718-1720	VBZ	denotes	is
T577	1721-1734	VBN	denotes	characterized
T579	1735-1737	IN	denotes	by
T580	1738-1745	JJ	denotes	corneal
T581	1746-1754	NN	denotes	clouding
T582	1754-1756	,	denotes	,
T583	1756-1763	JJ	denotes	delayed
T585	1764-1775	NN	denotes	psychomotor
T584	1776-1787	NN	denotes	development
T586	1787-1789	,	denotes	,
T587	1789-1792	CC	denotes	and
T588	1793-1799	JJ	denotes	mental
T589	1800-1811	NN	denotes	retardation
T590	1812-1816	WDT	denotes	that
T592	1817-1824	RB	denotes	usually
T591	1825-1833	VBZ	denotes	presents
T593	1834-1840	IN	denotes	during
T594	1841-1844	DT	denotes	the
T596	1845-1850	JJ	denotes	first
T595	1851-1855	NN	denotes	year
T597	1856-1858	IN	denotes	of
T598	1859-1863	NN	denotes	life
T599	1864-1865	-LRB-	denotes	[
T600	1865-1866	CD	denotes	1
T601	1866-1867	-RRB-	denotes	]
T602	1867-1868	.	denotes	.
T603	1868-1997	sentence	denotes	Another interesting clinical characteristic is that patients are constitutively achlorhydric with associated hypergastremia [2].
T604	1869-1876	DT	denotes	Another
T606	1877-1888	JJ	denotes	interesting
T607	1889-1897	JJ	denotes	clinical
T605	1898-1912	NN	denotes	characteristic
T608	1913-1915	VBZ	denotes	is
T609	1916-1920	IN	denotes	that
T611	1921-1929	NNS	denotes	patients
T610	1930-1933	VBP	denotes	are
T612	1934-1948	RB	denotes	constitutively
T613	1949-1961	JJ	denotes	achlorhydric
T614	1962-1966	IN	denotes	with
T615	1967-1977	VBN	denotes	associated
T616	1978-1992	NN	denotes	hypergastremia
T617	1993-1994	-LRB-	denotes	[
T618	1994-1995	CD	denotes	2
T619	1995-1996	-RRB-	denotes	]
T620	1996-1997	.	denotes	.
T621	1997-2122	sentence	denotes	Patients with MLIV do not show mucopolysaccharide excretion, skeletal changes, or organomegaly like the other mucolipidoses.
T622	1998-2006	NNS	denotes	Patients
T624	2007-2011	IN	denotes	with
T625	2012-2016	NN	denotes	MLIV
T626	2017-2019	VBP	denotes	do
T627	2020-2023	RB	denotes	not
T623	2024-2028	VB	denotes	show
T628	2029-2047	NN	denotes	mucopolysaccharide
T629	2048-2057	NN	denotes	excretion
T630	2057-2059	,	denotes	,
T631	2059-2067	JJ	denotes	skeletal
T632	2068-2075	NNS	denotes	changes
T633	2075-2077	,	denotes	,
T634	2077-2079	CC	denotes	or
T635	2080-2092	RB	denotes	organomegaly
T636	2093-2097	IN	denotes	like
T637	2098-2101	DT	denotes	the
T639	2102-2107	JJ	denotes	other
T638	2108-2121	NNS	denotes	mucolipidoses
T640	2121-2122	.	denotes	.
T641	2122-2330	sentence	denotes	Abnormal lysosomal storage bodies and large vacuoles have been found in skin and conjuctival biopsies using electron-microscopy and, prior to gene identification, served as the only means of diagnosis [3-5].
T642	2123-2131	JJ	denotes	Abnormal
T644	2132-2141	JJ	denotes	lysosomal
T645	2142-2149	NN	denotes	storage
T643	2150-2156	NNS	denotes	bodies
T647	2157-2160	CC	denotes	and
T648	2161-2166	JJ	denotes	large
T649	2167-2175	NNS	denotes	vacuoles
T650	2176-2180	VBP	denotes	have
T651	2181-2185	VBN	denotes	been
T646	2186-2191	VBN	denotes	found
T652	2192-2194	IN	denotes	in
T653	2195-2199	NN	denotes	skin
T655	2200-2203	CC	denotes	and
T656	2204-2215	JJ	denotes	conjuctival
T654	2216-2224	NNS	denotes	biopsies
T657	2225-2230	VBG	denotes	using
T658	2231-2239	NN	denotes	electron
T660	2239-2240	HYPH	denotes	-
T659	2240-2250	NN	denotes	microscopy
T661	2251-2254	CC	denotes	and
T662	2254-2256	,	denotes	,
T663	2256-2261	JJ	denotes	prior
T664	2262-2264	IN	denotes	to
T665	2265-2269	NN	denotes	gene
T666	2270-2284	NN	denotes	identification
T667	2284-2286	,	denotes	,
T668	2286-2292	VBD	denotes	served
T669	2293-2295	IN	denotes	as
T670	2296-2299	DT	denotes	the
T672	2300-2304	JJ	denotes	only
T671	2305-2310	NN	denotes	means
T673	2311-2313	IN	denotes	of
T674	2314-2323	NN	denotes	diagnosis
T675	2324-2325	-LRB-	denotes	[
T676	2325-2326	CD	denotes	3
T677	2326-2327	SYM	denotes	-
T678	2327-2328	CD	denotes	5
T679	2328-2329	-RRB-	denotes	]
T680	2329-2330	.	denotes	.
T681	2330-2513	sentence	denotes	A recent report estimates that the carrier frequency of MLIV in the Ashkenazi Jewish population is 1 in 100, and mutations have been reported in Jewish and non-Jewish families [6-9].
T682	2331-2332	DT	denotes	A
T684	2333-2339	JJ	denotes	recent
T683	2340-2346	NN	denotes	report
T685	2347-2356	VBZ	denotes	estimates
T686	2357-2361	IN	denotes	that
T688	2362-2365	DT	denotes	the
T690	2366-2373	NN	denotes	carrier
T689	2374-2383	NN	denotes	frequency
T691	2384-2386	IN	denotes	of
T692	2387-2391	NN	denotes	MLIV
T693	2392-2394	IN	denotes	in
T694	2395-2398	DT	denotes	the
T696	2399-2408	NNP	denotes	Ashkenazi
T697	2409-2415	JJ	denotes	Jewish
T695	2416-2426	NN	denotes	population
T687	2427-2429	VBZ	denotes	is
T698	2430-2431	CD	denotes	1
T699	2432-2434	IN	denotes	in
T700	2435-2438	CD	denotes	100
T701	2438-2440	,	denotes	,
T702	2440-2443	CC	denotes	and
T703	2444-2453	NNS	denotes	mutations
T705	2454-2458	VBP	denotes	have
T706	2459-2463	VBN	denotes	been
T704	2464-2472	VBN	denotes	reported
T707	2473-2475	IN	denotes	in
T708	2476-2482	JJ	denotes	Jewish
T710	2483-2486	CC	denotes	and
T711	2487-2497	JJ	denotes	non-Jewish
T709	2498-2506	NNS	denotes	families
T712	2507-2508	-LRB-	denotes	[
T713	2508-2509	CD	denotes	6
T714	2509-2510	SYM	denotes	-
T715	2510-2511	CD	denotes	9
T716	2511-2512	-RRB-	denotes	]
T717	2512-2513	.	denotes	.
T718	2513-2714	sentence	denotes	The human gene MCOLN1 (GenBank #AF287270) maps to chromosome 19p13.2-13.3 and encodes a novel protein that is a member of the transient receptor potential (TRP) cation channel gene superfamily [7-10].
T719	2514-2517	DT	denotes	The
T721	2518-2523	JJ	denotes	human
T720	2524-2528	NN	denotes	gene
T723	2529-2535	NN	denotes	MCOLN1
T724	2536-2537	-LRB-	denotes	(
T726	2537-2544	NNP	denotes	GenBank
T727	2545-2546	NN	denotes	#
T725	2546-2554	NN	denotes	AF287270
T728	2554-2555	-RRB-	denotes	)
T722	2556-2560	VBZ	denotes	maps
T729	2561-2563	IN	denotes	to
T730	2564-2574	NN	denotes	chromosome
T731	2575-2582	CD	denotes	19p13.2
T732	2582-2583	SYM	denotes	-
T733	2583-2587	CD	denotes	13.3
T734	2588-2591	CC	denotes	and
T735	2592-2599	VBZ	denotes	encodes
T736	2600-2601	DT	denotes	a
T738	2602-2607	JJ	denotes	novel
T737	2608-2615	NN	denotes	protein
T739	2616-2620	WDT	denotes	that
T740	2621-2623	VBZ	denotes	is
T741	2624-2625	DT	denotes	a
T742	2626-2632	NN	denotes	member
T743	2633-2635	IN	denotes	of
T744	2636-2639	DT	denotes	the
T746	2640-2649	JJ	denotes	transient
T748	2650-2658	NN	denotes	receptor
T747	2659-2668	NN	denotes	potential
T749	2669-2670	-LRB-	denotes	(
T750	2670-2673	NN	denotes	TRP
T751	2673-2674	-RRB-	denotes	)
T752	2675-2681	NN	denotes	cation
T753	2682-2689	NN	denotes	channel
T754	2690-2694	NN	denotes	gene
T745	2695-2706	NN	denotes	superfamily
T755	2707-2708	-LRB-	denotes	[
T756	2708-2709	CD	denotes	7
T757	2709-2710	SYM	denotes	-
T758	2710-2712	CD	denotes	10
T759	2712-2713	-RRB-	denotes	]
T760	2713-2714	.	denotes	.
T761	2714-2923	sentence	denotes	Protein trafficking studies suggest that MLIV is the result of a defect in the late endocytic pathway, contrary to the other mucolipidoses which are typically caused by defective lysosomal hydrolases [11,12].
T762	2715-2722	NN	denotes	Protein
T763	2723-2734	NN	denotes	trafficking
T764	2735-2742	NNS	denotes	studies
T765	2743-2750	VBP	denotes	suggest
T766	2751-2755	IN	denotes	that
T768	2756-2760	NN	denotes	MLIV
T767	2761-2763	VBZ	denotes	is
T769	2764-2767	DT	denotes	the
T770	2768-2774	NN	denotes	result
T771	2775-2777	IN	denotes	of
T772	2778-2779	DT	denotes	a
T773	2780-2786	NN	denotes	defect
T774	2787-2789	IN	denotes	in
T775	2790-2793	DT	denotes	the
T777	2794-2798	JJ	denotes	late
T778	2799-2808	JJ	denotes	endocytic
T776	2809-2816	NN	denotes	pathway
T779	2816-2818	,	denotes	,
T780	2818-2826	JJ	denotes	contrary
T781	2827-2829	IN	denotes	to
T782	2830-2833	DT	denotes	the
T784	2834-2839	JJ	denotes	other
T783	2840-2853	NNS	denotes	mucolipidoses
T785	2854-2859	WDT	denotes	which
T787	2860-2863	VBP	denotes	are
T788	2864-2873	RB	denotes	typically
T786	2874-2880	VBN	denotes	caused
T789	2881-2883	IN	denotes	by
T790	2884-2893	JJ	denotes	defective
T792	2894-2903	JJ	denotes	lysosomal
T791	2904-2914	NNS	denotes	hydrolases
T793	2915-2916	-LRB-	denotes	[
T795	2916-2918	CD	denotes	11
T796	2918-2919	,	denotes	,
T794	2919-2921	CD	denotes	12
T797	2921-2922	-RRB-	denotes	]
T798	2922-2923	.	denotes	.
T799	2923-2987	sentence	denotes	Recent work in Caenorhabditis elegans supports this hypothesis.
T800	2924-2930	JJ	denotes	Recent
T801	2931-2935	NN	denotes	work
T803	2936-2938	IN	denotes	in
T804	2939-2953	NNP	denotes	Caenorhabditis
T805	2954-2961	NNP	denotes	elegans
T802	2962-2970	VBZ	denotes	supports
T806	2971-2975	DT	denotes	this
T807	2976-2986	NN	denotes	hypothesis
T808	2986-2987	.	denotes	.
T809	2987-3250	sentence	denotes	Loss of function mutants of the MCOLN1 C. elegans homologue, cup-5, result in an increased rate of endocytosis, accumulation of large vacuoles, and a decreased rate of endocytosed protein breakdown; while over-expression of this gene reverses the phenotype [13].
T810	2988-2992	NN	denotes	Loss
T812	2993-2995	IN	denotes	of
T813	2996-3004	NN	denotes	function
T811	3005-3012	NNS	denotes	mutants
T815	3013-3015	IN	denotes	of
T816	3016-3019	DT	denotes	the
T818	3020-3026	NN	denotes	MCOLN1
T820	3027-3029	NNP	denotes	C.
T819	3030-3037	NNP	denotes	elegans
T817	3038-3047	NN	denotes	homologue
T821	3047-3049	,	denotes	,
T822	3049-3052	NN	denotes	cup
T823	3052-3053	HYPH	denotes	-
T824	3053-3054	CD	denotes	5
T825	3054-3056	,	denotes	,
T814	3056-3062	VBP	denotes	result
T826	3063-3065	IN	denotes	in
T827	3066-3068	DT	denotes	an
T829	3069-3078	VBN	denotes	increased
T828	3079-3083	NN	denotes	rate
T830	3084-3086	IN	denotes	of
T831	3087-3098	NN	denotes	endocytosis
T832	3098-3100	,	denotes	,
T833	3100-3112	NN	denotes	accumulation
T834	3113-3115	IN	denotes	of
T835	3116-3121	JJ	denotes	large
T836	3122-3130	NNS	denotes	vacuoles
T837	3130-3132	,	denotes	,
T838	3132-3135	CC	denotes	and
T839	3136-3137	DT	denotes	a
T841	3138-3147	VBN	denotes	decreased
T840	3148-3152	NN	denotes	rate
T842	3153-3155	IN	denotes	of
T843	3156-3167	VBN	denotes	endocytosed
T844	3168-3175	NN	denotes	protein
T845	3176-3185	NN	denotes	breakdown
T846	3185-3186	:	denotes	;
T847	3187-3192	IN	denotes	while
T849	3193-3208	NN	denotes	over-expression
T850	3209-3211	IN	denotes	of
T851	3212-3216	DT	denotes	this
T852	3217-3221	NN	denotes	gene
T848	3222-3230	VBZ	denotes	reverses
T853	3231-3234	DT	denotes	the
T854	3235-3244	NN	denotes	phenotype
T855	3245-3246	-LRB-	denotes	[
T856	3246-3248	CD	denotes	13
T857	3248-3249	-RRB-	denotes	]
T858	3249-3250	.	denotes	.
T859	3250-3399	sentence	denotes	Cloning and characterization of the mouse homologue of MCOLN1 is crucial for the development of mouse models of MLIV to further study this disorder.
T860	3251-3258	NN	denotes	Cloning
T862	3259-3262	CC	denotes	and
T863	3263-3279	NN	denotes	characterization
T864	3280-3282	IN	denotes	of
T865	3283-3286	DT	denotes	the
T867	3287-3292	NN	denotes	mouse
T866	3293-3302	NN	denotes	homologue
T868	3303-3305	IN	denotes	of
T869	3306-3312	NN	denotes	MCOLN1
T861	3313-3315	VBZ	denotes	is
T870	3316-3323	JJ	denotes	crucial
T871	3324-3327	IN	denotes	for
T872	3328-3331	DT	denotes	the
T873	3332-3343	NN	denotes	development
T874	3344-3346	IN	denotes	of
T875	3347-3352	NN	denotes	mouse
T876	3353-3359	NNS	denotes	models
T877	3360-3362	IN	denotes	of
T878	3363-3367	NN	denotes	MLIV
T879	3368-3370	TO	denotes	to
T881	3371-3378	RB	denotes	further
T880	3379-3384	VB	denotes	study
T882	3385-3389	DT	denotes	this
T883	3390-3398	NN	denotes	disorder
T884	3398-3399	.	denotes	.
T1037	3437-3444	NN	denotes	mapping
T1038	3445-3447	IN	denotes	of
T1039	3448-3451	DT	denotes	the
T1041	3452-3457	NN	denotes	mouse
T1040	3458-3467	NN	denotes	homologue
T1042	3468-3474	NN	denotes	Mcoln1
T1043	3474-3709	sentence	denotes	In order to clone the mouse homologue of MCOLN1, the human amino acid sequence was compared to the high throughput genomic sequence (HTGS) database using TBLASTN, which identified the mouse BAC clone RPCI-23_387H4 (GB No. AC079544.1).
T1044	3475-3477	IN	denotes	In
T1046	3478-3483	NN	denotes	order
T1047	3484-3486	TO	denotes	to
T1048	3487-3492	VB	denotes	clone
T1049	3493-3496	DT	denotes	the
T1051	3497-3502	NN	denotes	mouse
T1050	3503-3512	NN	denotes	homologue
T1052	3513-3515	IN	denotes	of
T1053	3516-3522	NN	denotes	MCOLN1
T1054	3522-3524	,	denotes	,
T1055	3524-3527	DT	denotes	the
T1057	3528-3533	JJ	denotes	human
T1058	3534-3539	NN	denotes	amino
T1059	3540-3544	NN	denotes	acid
T1056	3545-3553	NN	denotes	sequence
T1060	3554-3557	VBD	denotes	was
T1045	3558-3566	VBN	denotes	compared
T1061	3567-3569	IN	denotes	to
T1062	3570-3573	DT	denotes	the
T1064	3574-3578	JJ	denotes	high
T1065	3579-3589	NN	denotes	throughput
T1067	3590-3597	JJ	denotes	genomic
T1066	3598-3606	NN	denotes	sequence
T1068	3607-3608	-LRB-	denotes	(
T1069	3608-3612	NN	denotes	HTGS
T1070	3612-3613	-RRB-	denotes	)
T1063	3614-3622	NN	denotes	database
T1071	3623-3628	VBG	denotes	using
T1072	3629-3636	NN	denotes	TBLASTN
T1073	3636-3638	,	denotes	,
T1074	3638-3643	WDT	denotes	which
T1075	3644-3654	VBD	denotes	identified
T1076	3655-3658	DT	denotes	the
T1078	3659-3664	NN	denotes	mouse
T1079	3665-3668	NN	denotes	BAC
T1077	3669-3674	NN	denotes	clone
T1080	3675-3679	NN	denotes	RPCI
T1082	3679-3680	HYPH	denotes	-
T1081	3680-3688	NN	denotes	23_387H4
T1083	3689-3690	-LRB-	denotes	(
T1085	3690-3692	NN	denotes	GB
T1086	3693-3696	NN	denotes	No.
T1084	3697-3707	NN	denotes	AC079544.1
T1087	3707-3708	-RRB-	denotes	)
T1088	3708-3709	.	denotes	.
T1089	3709-3961	sentence	denotes	Correspondence with the Joint Genome Institute and the Lawrence Livermore National Laboratory (LLNL) Human Genome Center confirmed the location of this BAC to mouse chromosome 8 and allowed us to construct a physical map of this region [14] (Fig. 1A).
T1090	3710-3724	NN	denotes	Correspondence
T1092	3725-3729	IN	denotes	with
T1093	3730-3733	DT	denotes	the
T1095	3734-3739	NNP	denotes	Joint
T1096	3740-3746	NNP	denotes	Genome
T1094	3747-3756	NNP	denotes	Institute
T1097	3757-3760	CC	denotes	and
T1098	3761-3764	DT	denotes	the
T1100	3765-3773	NNP	denotes	Lawrence
T1101	3774-3783	NNP	denotes	Livermore
T1102	3784-3792	NNP	denotes	National
T1099	3793-3803	NNP	denotes	Laboratory
T1104	3804-3805	-LRB-	denotes	(
T1105	3805-3809	NNP	denotes	LLNL
T1106	3809-3810	-RRB-	denotes	)
T1107	3811-3816	NNP	denotes	Human
T1108	3817-3823	NNP	denotes	Genome
T1103	3824-3830	NNP	denotes	Center
T1091	3831-3840	VBD	denotes	confirmed
T1109	3841-3844	DT	denotes	the
T1110	3845-3853	NN	denotes	location
T1111	3854-3856	IN	denotes	of
T1112	3857-3861	DT	denotes	this
T1113	3862-3865	NN	denotes	BAC
T1114	3866-3868	IN	denotes	to
T1115	3869-3874	NN	denotes	mouse
T1116	3875-3885	NN	denotes	chromosome
T1117	3886-3887	CD	denotes	8
T1118	3888-3891	CC	denotes	and
T1119	3892-3899	VBD	denotes	allowed
T1120	3900-3902	PRP	denotes	us
T1122	3903-3905	TO	denotes	to
T1121	3906-3915	VB	denotes	construct
T1123	3916-3917	DT	denotes	a
T1125	3918-3926	JJ	denotes	physical
T1124	3927-3930	NN	denotes	map
T1126	3931-3933	IN	denotes	of
T1127	3934-3938	DT	denotes	this
T1128	3939-3945	NN	denotes	region
T1129	3946-3947	-LRB-	denotes	[
T1130	3947-3949	CD	denotes	14
T1131	3949-3950	-RRB-	denotes	]
T1132	3951-3952	-LRB-	denotes	(
T1134	3952-3956	NN	denotes	Fig.
T1133	3957-3959	NN	denotes	1A
T1135	3959-3960	-RRB-	denotes	)
T1136	3960-3961	.	denotes	.
T1137	3961-4113	sentence	denotes	The BAC sequence was then compared to the mouse EST database using BLASTN, and multiple ESTs and their corresponding I.M.A.G.E. clones were identified.
T1138	3962-3965	DT	denotes	The
T1140	3966-3969	NN	denotes	BAC
T1139	3970-3978	NN	denotes	sequence
T1142	3979-3982	VBD	denotes	was
T1143	3983-3987	RB	denotes	then
T1141	3988-3996	VBN	denotes	compared
T1144	3997-3999	IN	denotes	to
T1145	4000-4003	DT	denotes	the
T1147	4004-4009	NN	denotes	mouse
T1148	4010-4013	NN	denotes	EST
T1146	4014-4022	NN	denotes	database
T1149	4023-4028	VBG	denotes	using
T1150	4029-4035	NN	denotes	BLASTN
T1151	4035-4037	,	denotes	,
T1152	4037-4040	CC	denotes	and
T1153	4041-4049	JJ	denotes	multiple
T1154	4050-4054	NNS	denotes	ESTs
T1156	4055-4058	CC	denotes	and
T1157	4059-4064	PRP$	denotes	their
T1159	4065-4078	VBG	denotes	corresponding
T1160	4079-4089	NNP	denotes	I.M.A.G.E.
T1158	4090-4096	NNS	denotes	clones
T1161	4097-4101	VBD	denotes	were
T1155	4102-4112	VBN	denotes	identified
T1162	4112-4113	.	denotes	.
T1163	4113-5069	sentence	denotes	Figure 1 Physical and transcript map of the Mcoln1 gene region. (A) Physical map showing a 590 kb segment of mouse chromosome 8 syntenous to human chromosome 19, anchored by the genes Insr and Nte. The region is covered by three BACs: RPCI-23_334D24 (AC087153), RPCI-23_312B8 (AC087150), and RPCI-23_387H4 (AC079544). (B) The transcript map of Mcoln1 shows 14 exons, and the locations of the probes used in this study are illustrated. The map also shows the expanded exon 13 in the alternatively spliced transcript (hashed box). UniGene cluster Mm.39099 is the homologue of the human zinc finger gene (AC001252). It should be noted that the scale of the transcript map is in reference to the 201 kb scale of BAC RPCI-23_387H4. Three clones (ID Nos. 604971, 1228665, 1247566) from the UniGene cluster Mm. 8356 were obtained, sequenced, and assembled into a 2 kb transcript with an open reading frame of 580 amino acids designated Mcoln1 (GB No. AF302010).
T4216	4124-4132	JJ	denotes	Physical
T4218	4133-4136	CC	denotes	and
T4219	4137-4147	NN	denotes	transcript
T4217	4148-4151	NN	denotes	map
T4220	4152-4154	IN	denotes	of
T4221	4155-4158	DT	denotes	the
T4223	4159-4165	NN	denotes	Mcoln1
T4224	4166-4170	NN	denotes	gene
T4222	4171-4177	NN	denotes	region
T4225	4177-4178	.	denotes	.
T4226	4178-4312	sentence	denotes	(A) Physical map showing a 590 kb segment of mouse chromosome 8 syntenous to human chromosome 19, anchored by the genes Insr and Nte.
T4227	4179-4180	-LRB-	denotes	(
T4228	4180-4181	LS	denotes	A
T4230	4181-4182	-RRB-	denotes	)
T4231	4183-4191	JJ	denotes	Physical
T4229	4192-4195	NN	denotes	map
T4232	4196-4203	VBG	denotes	showing
T4233	4204-4205	DT	denotes	a
T4235	4206-4209	CD	denotes	590
T4236	4210-4212	NN	denotes	kb
T4234	4213-4220	NN	denotes	segment
T4237	4221-4223	IN	denotes	of
T4238	4224-4229	NN	denotes	mouse
T4239	4230-4240	NN	denotes	chromosome
T4240	4241-4242	CD	denotes	8
T4241	4243-4252	JJ	denotes	syntenous
T4242	4253-4255	IN	denotes	to
T4243	4256-4261	JJ	denotes	human
T4244	4262-4272	NN	denotes	chromosome
T4245	4273-4275	CD	denotes	19
T4246	4275-4277	,	denotes	,
T4247	4277-4285	VBN	denotes	anchored
T4248	4286-4288	IN	denotes	by
T4249	4289-4292	DT	denotes	the
T4251	4293-4298	NNS	denotes	genes
T4250	4299-4303	NNP	denotes	Insr
T4252	4304-4307	CC	denotes	and
T4253	4308-4311	NNP	denotes	Nte
T4254	4311-4312	.	denotes	.
T4255	4312-4432	sentence	denotes	The region is covered by three BACs: RPCI-23_334D24 (AC087153), RPCI-23_312B8 (AC087150), and RPCI-23_387H4 (AC079544).
T4256	4313-4316	DT	denotes	The
T4257	4317-4323	NN	denotes	region
T4259	4324-4326	VBZ	denotes	is
T4258	4327-4334	VBN	denotes	covered
T4260	4335-4337	IN	denotes	by
T4261	4338-4343	CD	denotes	three
T4262	4344-4348	NNS	denotes	BACs
T4263	4348-4350	:	denotes	:
T4264	4350-4354	NN	denotes	RPCI
T4266	4354-4355	HYPH	denotes	-
T4265	4355-4364	NN	denotes	23_334D24
T4267	4365-4366	-LRB-	denotes	(
T4268	4366-4374	NN	denotes	AC087153
T4269	4374-4375	-RRB-	denotes	)
T4270	4375-4377	,	denotes	,
T4271	4377-4381	NN	denotes	RPCI
T4273	4381-4382	HYPH	denotes	-
T4272	4382-4390	NN	denotes	23_312B8
T4274	4391-4392	-LRB-	denotes	(
T4275	4392-4400	NN	denotes	AC087150
T4276	4400-4401	-RRB-	denotes	)
T4277	4401-4403	,	denotes	,
T4278	4403-4406	CC	denotes	and
T4279	4407-4411	NN	denotes	RPCI
T4281	4411-4412	HYPH	denotes	-
T4280	4412-4420	NN	denotes	23_387H4
T4282	4421-4422	-LRB-	denotes	(
T4283	4422-4430	NN	denotes	AC079544
T4284	4430-4431	-RRB-	denotes	)
T4285	4431-4432	.	denotes	.
T4286	4432-4549	sentence	denotes	(B) The transcript map of Mcoln1 shows 14 exons, and the locations of the probes used in this study are illustrated.
T4287	4433-4434	-LRB-	denotes	(
T4288	4434-4435	LS	denotes	B
T4290	4435-4436	-RRB-	denotes	)
T4291	4437-4440	DT	denotes	The
T4293	4441-4451	NN	denotes	transcript
T4292	4452-4455	NN	denotes	map
T4294	4456-4458	IN	denotes	of
T4295	4459-4465	NN	denotes	Mcoln1
T4289	4466-4471	VBZ	denotes	shows
T4296	4472-4474	CD	denotes	14
T4297	4475-4480	NNS	denotes	exons
T4298	4480-4482	,	denotes	,
T4299	4482-4485	CC	denotes	and
T4300	4486-4489	DT	denotes	the
T4301	4490-4499	NNS	denotes	locations
T4303	4500-4502	IN	denotes	of
T4304	4503-4506	DT	denotes	the
T4305	4507-4513	NNS	denotes	probes
T4306	4514-4518	VBN	denotes	used
T4307	4519-4521	IN	denotes	in
T4308	4522-4526	DT	denotes	this
T4309	4527-4532	NN	denotes	study
T4310	4533-4536	VBP	denotes	are
T4302	4537-4548	VBN	denotes	illustrated
T4311	4548-4549	.	denotes	.
T4312	4549-4643	sentence	denotes	The map also shows the expanded exon 13 in the alternatively spliced transcript (hashed box).
T4313	4550-4553	DT	denotes	The
T4314	4554-4557	NN	denotes	map
T4316	4558-4562	RB	denotes	also
T4315	4563-4568	VBZ	denotes	shows
T4317	4569-4572	DT	denotes	the
T4319	4573-4581	VBN	denotes	expanded
T4318	4582-4586	NN	denotes	exon
T4320	4587-4589	CD	denotes	13
T4321	4590-4592	IN	denotes	in
T4322	4593-4596	DT	denotes	the
T4324	4597-4610	RB	denotes	alternatively
T4325	4611-4618	VBN	denotes	spliced
T4323	4619-4629	NN	denotes	transcript
T4326	4630-4631	-LRB-	denotes	(
T4328	4631-4637	VBN	denotes	hashed
T4327	4638-4641	NN	denotes	box
T4329	4641-4642	-RRB-	denotes	)
T4330	4642-4643	.	denotes	.
T4331	4643-4727	sentence	denotes	UniGene cluster Mm.39099 is the homologue of the human zinc finger gene (AC001252).
T4332	4644-4651	NN	denotes	UniGene
T4334	4652-4659	NN	denotes	cluster
T4333	4660-4668	NN	denotes	Mm.39099
T4335	4669-4671	VBZ	denotes	is
T4336	4672-4675	DT	denotes	the
T4337	4676-4685	NN	denotes	homologue
T4338	4686-4688	IN	denotes	of
T4339	4689-4692	DT	denotes	the
T4341	4693-4698	JJ	denotes	human
T4342	4699-4703	NN	denotes	zinc
T4343	4704-4710	NN	denotes	finger
T4340	4711-4715	NN	denotes	gene
T4344	4716-4717	-LRB-	denotes	(
T4345	4717-4725	NN	denotes	AC001252
T4346	4725-4726	-RRB-	denotes	)
T4347	4726-4727	.	denotes	.
T4348	4727-4841	sentence	denotes	It should be noted that the scale of the transcript map is in reference to the 201 kb scale of BAC RPCI-23_387H4.
T4349	4728-4730	PRP	denotes	It
T4351	4731-4737	MD	denotes	should
T4352	4738-4740	VB	denotes	be
T4350	4741-4746	VBN	denotes	noted
T4353	4747-4751	IN	denotes	that
T4355	4752-4755	DT	denotes	the
T4356	4756-4761	NN	denotes	scale
T4357	4762-4764	IN	denotes	of
T4358	4765-4768	DT	denotes	the
T4360	4769-4779	NN	denotes	transcript
T4359	4780-4783	NN	denotes	map
T4354	4784-4786	VBZ	denotes	is
T4361	4787-4789	IN	denotes	in
T4362	4790-4799	NN	denotes	reference
T4363	4800-4802	IN	denotes	to
T4364	4803-4806	DT	denotes	the
T4366	4807-4810	CD	denotes	201
T4367	4811-4813	NN	denotes	kb
T4365	4814-4819	NN	denotes	scale
T4368	4820-4822	IN	denotes	of
T4369	4823-4826	NN	denotes	BAC
T4371	4827-4831	NN	denotes	RPCI
T4372	4831-4832	HYPH	denotes	-
T4370	4832-4840	NN	denotes	23_387H4
T4373	4840-4841	.	denotes	.
T1164	4842-4847	CD	denotes	Three
T1165	4848-4854	NNS	denotes	clones
T1167	4855-4856	-LRB-	denotes	(
T1169	4856-4858	NNP	denotes	ID
T1170	4859-4863	NNP	denotes	Nos.
T1171	4864-4870	CD	denotes	604971
T1172	4870-4872	,	denotes	,
T1173	4872-4879	CD	denotes	1228665
T1174	4879-4881	,	denotes	,
T1168	4881-4888	CD	denotes	1247566
T1175	4888-4889	-RRB-	denotes	)
T1176	4890-4894	IN	denotes	from
T1177	4895-4898	DT	denotes	the
T1179	4899-4906	NNP	denotes	UniGene
T1178	4907-4914	NNP	denotes	cluster
T1180	4915-4918	NNP	denotes	Mm.
T1181	4919-4923	CD	denotes	8356
T1182	4924-4928	VBD	denotes	were
T1166	4929-4937	VBN	denotes	obtained
T1183	4937-4939	,	denotes	,
T1184	4939-4948	VBN	denotes	sequenced
T1185	4948-4950	,	denotes	,
T1186	4950-4953	CC	denotes	and
T1187	4954-4963	VBD	denotes	assembled
T1188	4964-4968	IN	denotes	into
T1189	4969-4970	DT	denotes	a
T1191	4971-4972	CD	denotes	2
T1192	4973-4975	NN	denotes	kb
T1190	4976-4986	NN	denotes	transcript
T1193	4987-4991	IN	denotes	with
T1194	4992-4994	DT	denotes	an
T1196	4995-4999	JJ	denotes	open
T1197	5000-5007	NN	denotes	reading
T1195	5008-5013	NN	denotes	frame
T1198	5014-5016	IN	denotes	of
T1199	5017-5020	CD	denotes	580
T1201	5021-5026	NN	denotes	amino
T1200	5027-5032	NNS	denotes	acids
T1202	5033-5043	VBN	denotes	designated
T1203	5044-5050	NN	denotes	Mcoln1
T1204	5051-5052	-LRB-	denotes	(
T1206	5052-5054	NN	denotes	GB
T1207	5055-5058	NN	denotes	No.
T1205	5059-5067	NN	denotes	AF302010
T1208	5067-5068	-RRB-	denotes	)
T1209	5068-5069	.	denotes	.
T1210	5069-5161	sentence	denotes	Comparison of the cDNA sequence to the BAC clone showed that the gene consists of 14 exons.
T1211	5070-5080	NN	denotes	Comparison
T1213	5081-5083	IN	denotes	of
T1214	5084-5087	DT	denotes	the
T1216	5088-5092	NN	denotes	cDNA
T1215	5093-5101	NN	denotes	sequence
T1217	5102-5104	IN	denotes	to
T1218	5105-5108	DT	denotes	the
T1220	5109-5112	NN	denotes	BAC
T1219	5113-5118	NN	denotes	clone
T1212	5119-5125	VBD	denotes	showed
T1221	5126-5130	IN	denotes	that
T1223	5131-5134	DT	denotes	the
T1224	5135-5139	NN	denotes	gene
T1222	5140-5148	VBZ	denotes	consists
T1225	5149-5151	IN	denotes	of
T1226	5152-5154	CD	denotes	14
T1227	5155-5160	NNS	denotes	exons
T1228	5160-5161	.	denotes	.
T1229	5161-5577	sentence	denotes	We then created a transcript map of the Mcoln1 region of mouse chromosome 8 (Fig. 1B), noting the presence of the UniGene Cluster Mm. 39099, a homologue of the human zinc finger gene (GB No. AC001252) that terminates approximately 1.8 kb before the start of Mcoln1; and Nte, the mouse homologue of the human neuropathy target esterase gene (NTE) beginning 130 base pairs after the polyadenylation signal for Mcoln1.
T1230	5162-5164	PRP	denotes	We
T1232	5165-5169	RB	denotes	then
T1231	5170-5177	VBD	denotes	created
T1233	5178-5179	DT	denotes	a
T1235	5180-5190	NN	denotes	transcript
T1234	5191-5194	NN	denotes	map
T1236	5195-5197	IN	denotes	of
T1237	5198-5201	DT	denotes	the
T1239	5202-5208	NN	denotes	Mcoln1
T1238	5209-5215	NN	denotes	region
T1240	5216-5218	IN	denotes	of
T1241	5219-5224	NN	denotes	mouse
T1242	5225-5235	NN	denotes	chromosome
T1243	5236-5237	CD	denotes	8
T1244	5238-5239	-LRB-	denotes	(
T1246	5239-5243	NN	denotes	Fig.
T1245	5244-5246	NN	denotes	1B
T1247	5246-5247	-RRB-	denotes	)
T1248	5247-5249	,	denotes	,
T1249	5249-5255	VBG	denotes	noting
T1250	5256-5259	DT	denotes	the
T1251	5260-5268	NN	denotes	presence
T1252	5269-5271	IN	denotes	of
T1253	5272-5275	DT	denotes	the
T1255	5276-5283	NNP	denotes	UniGene
T1254	5284-5291	NNP	denotes	Cluster
T1256	5292-5295	NNP	denotes	Mm.
T1257	5296-5301	CD	denotes	39099
T1258	5301-5303	,	denotes	,
T1259	5303-5304	DT	denotes	a
T1260	5305-5314	NN	denotes	homologue
T1261	5315-5317	IN	denotes	of
T1262	5318-5321	DT	denotes	the
T1264	5322-5327	JJ	denotes	human
T1265	5328-5332	NN	denotes	zinc
T1266	5333-5339	NN	denotes	finger
T1263	5340-5344	NN	denotes	gene
T1267	5345-5346	-LRB-	denotes	(
T1269	5346-5348	NN	denotes	GB
T1270	5349-5352	NN	denotes	No.
T1268	5353-5361	NN	denotes	AC001252
T1271	5361-5362	-RRB-	denotes	)
T1272	5363-5367	WDT	denotes	that
T1273	5368-5378	VBZ	denotes	terminates
T1274	5379-5392	RB	denotes	approximately
T1275	5393-5396	CD	denotes	1.8
T1276	5397-5399	NN	denotes	kb
T1277	5400-5406	IN	denotes	before
T1278	5407-5410	DT	denotes	the
T1279	5411-5416	NN	denotes	start
T1280	5417-5419	IN	denotes	of
T1281	5420-5426	NN	denotes	Mcoln1
T1282	5426-5427	:	denotes	;
T1283	5428-5431	CC	denotes	and
T1284	5432-5435	NNP	denotes	Nte
T1285	5435-5437	,	denotes	,
T1286	5437-5440	DT	denotes	the
T1288	5441-5446	NN	denotes	mouse
T1287	5447-5456	NN	denotes	homologue
T1289	5457-5459	IN	denotes	of
T1290	5460-5463	DT	denotes	the
T1292	5464-5469	JJ	denotes	human
T1293	5470-5480	NN	denotes	neuropathy
T1295	5481-5487	NN	denotes	target
T1294	5488-5496	NN	denotes	esterase
T1291	5497-5501	NN	denotes	gene
T1296	5502-5503	-LRB-	denotes	(
T1297	5503-5506	NN	denotes	NTE
T1298	5506-5507	-RRB-	denotes	)
T1299	5508-5517	VBG	denotes	beginning
T1300	5518-5521	CD	denotes	130
T1302	5522-5526	NN	denotes	base
T1301	5527-5532	NNS	denotes	pairs
T1303	5533-5538	IN	denotes	after
T1304	5539-5542	DT	denotes	the
T1306	5543-5558	NN	denotes	polyadenylation
T1305	5559-5565	NN	denotes	signal
T1307	5566-5569	IN	denotes	for
T1308	5570-5576	NN	denotes	Mcoln1
T1309	5576-5577	.	denotes	.
T1310	5577-5856	sentence	denotes	The transcript map of the region surrounding Mcoln1 is similar to the corresponding region of the homologous human MCOLN1 region [15], and the presence of the zinc finger gene and Nte confirms and extends the region of synteny between human chromosome 19 and mouse chromosome 8.
T1311	5578-5581	DT	denotes	The
T1313	5582-5592	NN	denotes	transcript
T1312	5593-5596	NN	denotes	map
T1315	5597-5599	IN	denotes	of
T1316	5600-5603	DT	denotes	the
T1317	5604-5610	NN	denotes	region
T1318	5611-5622	VBG	denotes	surrounding
T1319	5623-5629	NN	denotes	Mcoln1
T1314	5630-5632	VBZ	denotes	is
T1320	5633-5640	JJ	denotes	similar
T1321	5641-5643	IN	denotes	to
T1322	5644-5647	DT	denotes	the
T1324	5648-5661	JJ	denotes	corresponding
T1323	5662-5668	NN	denotes	region
T1325	5669-5671	IN	denotes	of
T1326	5672-5675	DT	denotes	the
T1328	5676-5686	JJ	denotes	homologous
T1329	5687-5692	JJ	denotes	human
T1330	5693-5699	NN	denotes	MCOLN1
T1327	5700-5706	NN	denotes	region
T1331	5707-5708	-LRB-	denotes	[
T1332	5708-5710	CD	denotes	15
T1333	5710-5711	-RRB-	denotes	]
T1334	5711-5713	,	denotes	,
T1335	5713-5716	CC	denotes	and
T1336	5717-5720	DT	denotes	the
T1337	5721-5729	NN	denotes	presence
T1339	5730-5732	IN	denotes	of
T1340	5733-5736	DT	denotes	the
T1342	5737-5741	NN	denotes	zinc
T1343	5742-5748	NN	denotes	finger
T1341	5749-5753	NN	denotes	gene
T1344	5754-5757	CC	denotes	and
T1345	5758-5761	NN	denotes	Nte
T1346	5762-5770	NNS	denotes	confirms
T1347	5771-5774	CC	denotes	and
T1338	5775-5782	VBZ	denotes	extends
T1348	5783-5786	DT	denotes	the
T1349	5787-5793	NN	denotes	region
T1350	5794-5796	IN	denotes	of
T1351	5797-5804	NN	denotes	synteny
T1352	5805-5812	IN	denotes	between
T1353	5813-5818	JJ	denotes	human
T1354	5819-5829	NN	denotes	chromosome
T1355	5830-5832	CD	denotes	19
T1356	5833-5836	CC	denotes	and
T1357	5837-5842	NN	denotes	mouse
T1358	5843-5853	NN	denotes	chromosome
T1359	5854-5855	CD	denotes	8
T1360	5855-5856	.	denotes	.
T1482	5858-5874	NN	denotes	Characterization
T1483	5875-5877	IN	denotes	of
T1484	5878-5884	NN	denotes	Mcoln1
T1485	5884-5966	sentence	denotes	Comparison of the mouse and human peptide sequences showed 91% identity (Fig. 2).
T1486	5885-5895	NN	denotes	Comparison
T1488	5896-5898	IN	denotes	of
T1489	5899-5902	DT	denotes	the
T1491	5903-5908	NN	denotes	mouse
T1492	5909-5912	CC	denotes	and
T1493	5913-5918	JJ	denotes	human
T1494	5919-5926	NN	denotes	peptide
T1490	5927-5936	NNS	denotes	sequences
T1487	5937-5943	VBD	denotes	showed
T1495	5944-5946	CD	denotes	91
T1496	5946-5947	NN	denotes	%
T1497	5948-5956	NN	denotes	identity
T1498	5957-5958	-LRB-	denotes	(
T1499	5958-5962	NN	denotes	Fig.
T1500	5963-5964	CD	denotes	2
T1501	5964-5965	-RRB-	denotes	)
T1502	5965-5966	.	denotes	.
T1503	5966-6148	sentence	denotes	The C. elegans homologue cup-5 shows 34% identity with Mcoln1 and BLASTP analysis of Mcoln1 identified a putative Drosophila melanogaster homologue that shows 38% identity (Fig. 2).
T1504	5967-5970	DT	denotes	The
T1506	5971-5973	NNP	denotes	C.
T1507	5974-5981	NNP	denotes	elegans
T1505	5982-5991	NN	denotes	homologue
T1509	5992-5995	NN	denotes	cup
T1510	5995-5996	HYPH	denotes	-
T1511	5996-5997	CD	denotes	5
T1508	5998-6003	VBZ	denotes	shows
T1512	6004-6006	CD	denotes	34
T1513	6006-6007	NN	denotes	%
T1514	6008-6016	NN	denotes	identity
T1515	6017-6021	IN	denotes	with
T1516	6022-6028	NN	denotes	Mcoln1
T1517	6029-6032	CC	denotes	and
T1518	6033-6039	NN	denotes	BLASTP
T1519	6040-6048	NN	denotes	analysis
T1521	6049-6051	IN	denotes	of
T1522	6052-6058	NN	denotes	Mcoln1
T1520	6059-6069	VBD	denotes	identified
T1523	6070-6071	DT	denotes	a
T1525	6072-6080	JJ	denotes	putative
T1526	6081-6091	NNP	denotes	Drosophila
T1527	6092-6104	NN	denotes	melanogaster
T1524	6105-6114	NN	denotes	homologue
T1528	6115-6119	WDT	denotes	that
T1529	6120-6125	VBZ	denotes	shows
T1530	6126-6128	CD	denotes	38
T1531	6128-6129	NN	denotes	%
T1532	6130-6138	NN	denotes	identity
T1533	6139-6140	-LRB-	denotes	(
T1534	6140-6144	NN	denotes	Fig.
T1535	6145-6146	CD	denotes	2
T1536	6146-6147	-RRB-	denotes	)
T1537	6147-6148	.	denotes	.
T1538	6148-6251	sentence	denotes	Interestingly, two MCOLN1 amino acid substitutions that result in MLIV occur at conserved amino acids.
T1539	6149-6162	RB	denotes	Interestingly
T1541	6162-6164	,	denotes	,
T1542	6164-6167	CD	denotes	two
T1544	6168-6174	NN	denotes	MCOLN1
T1545	6175-6180	NN	denotes	amino
T1546	6181-6185	NN	denotes	acid
T1543	6186-6199	NNS	denotes	substitutions
T1547	6200-6204	WDT	denotes	that
T1548	6205-6211	VBP	denotes	result
T1549	6212-6214	IN	denotes	in
T1550	6215-6219	NN	denotes	MLIV
T1540	6220-6225	VBP	denotes	occur
T1551	6226-6228	IN	denotes	at
T1552	6229-6238	VBN	denotes	conserved
T1554	6239-6244	NN	denotes	amino
T1553	6245-6250	NNS	denotes	acids
T1555	6250-6251	.	denotes	.
T1556	6251-6434	sentence	denotes	TMPred analysis predicts a protein structure that is nearly identical to MCOLN1, containing 6 transmembrane domains with the N- and C-termini residing in the cytoplasm (Fig. 2) [9].
T1557	6252-6258	VBN	denotes	TMPred
T1558	6259-6267	NN	denotes	analysis
T1559	6269-6277	VBZ	denotes	predicts
T1560	6278-6279	DT	denotes	a
T1562	6280-6287	NN	denotes	protein
T1561	6288-6297	NN	denotes	structure
T1563	6298-6302	WDT	denotes	that
T1564	6303-6305	VBZ	denotes	is
T1565	6306-6312	RB	denotes	nearly
T1566	6313-6322	JJ	denotes	identical
T1567	6323-6325	IN	denotes	to
T1568	6326-6332	NN	denotes	MCOLN1
T1569	6332-6334	,	denotes	,
T1570	6334-6344	VBG	denotes	containing
T1571	6345-6346	CD	denotes	6
T1573	6347-6360	NN	denotes	transmembrane
T1572	6361-6368	NNS	denotes	domains
T1574	6369-6373	IN	denotes	with
T1575	6374-6377	DT	denotes	the
T1577	6378-6379	NN	denotes	N
T1578	6379-6380	HYPH	denotes	-
T1579	6381-6384	CC	denotes	and
T1580	6385-6386	NN	denotes	C
T1581	6386-6387	HYPH	denotes	-
T1576	6387-6394	NNS	denotes	termini
T1582	6395-6403	VBG	denotes	residing
T1583	6404-6406	IN	denotes	in
T1584	6407-6410	DT	denotes	the
T1585	6411-6420	NN	denotes	cytoplasm
T1586	6421-6422	-LRB-	denotes	(
T1587	6422-6426	NN	denotes	Fig.
T1588	6427-6428	CD	denotes	2
T1589	6428-6429	-RRB-	denotes	)
T1590	6430-6431	-LRB-	denotes	[
T1591	6431-6432	CD	denotes	9
T1592	6432-6433	-RRB-	denotes	]
T1593	6433-6434	.	denotes	.
T4431	6445-6452	NN	denotes	Peptide
T4432	6453-6461	NN	denotes	sequence
T4433	6462-6472	NN	denotes	comparison
T4434	6473-6475	IN	denotes	of
T4435	6476-6482	NN	denotes	Mcoln1
T4436	6483-6485	IN	denotes	to
T4437	6486-6491	JJ	denotes	human
T4439	6491-6493	,	denotes	,
T4440	6493-6495	NNP	denotes	D.
T4441	6496-6508	NNP	denotes	melanogaster
T4442	6508-6510	,	denotes	,
T4443	6510-6513	CC	denotes	and
T4444	6514-6516	NNP	denotes	C.
T4445	6517-6524	NNP	denotes	elegans
T4446	6525-6526	-LRB-	denotes	(
T4447	6526-6529	NN	denotes	cup
T4449	6529-6530	HYPH	denotes	-
T4448	6530-6531	NN	denotes	5
T4450	6531-6532	-RRB-	denotes	)
T4438	6533-6543	NNS	denotes	homologues
T4451	6543-6544	.	denotes	.
T4452	6544-6717	sentence	denotes	Blue lines indicate transmembrane domains, the red box surrounds the putative channel pore, the orange box surrounds the putative late endosomal/lysosomal targeting signal.
T4453	6545-6549	JJ	denotes	Blue
T4454	6550-6555	NNS	denotes	lines
T4455	6556-6564	VBP	denotes	indicate
T4457	6565-6578	NN	denotes	transmembrane
T4458	6579-6586	NNS	denotes	domains
T4459	6586-6588	,	denotes	,
T4460	6588-6591	DT	denotes	the
T4462	6592-6595	JJ	denotes	red
T4461	6596-6599	NN	denotes	box
T4463	6600-6609	VBZ	denotes	surrounds
T4464	6610-6613	DT	denotes	the
T4466	6614-6622	JJ	denotes	putative
T4467	6623-6630	NN	denotes	channel
T4465	6631-6635	NN	denotes	pore
T4468	6635-6637	,	denotes	,
T4469	6637-6640	DT	denotes	the
T4471	6641-6647	NN	denotes	orange
T4470	6648-6651	NN	denotes	box
T4456	6652-6661	VBZ	denotes	surrounds
T4472	6662-6665	DT	denotes	the
T4474	6666-6674	JJ	denotes	putative
T4475	6675-6679	JJ	denotes	late
T4477	6680-6689	JJ	denotes	endosomal
T4479	6689-6690	HYPH	denotes	/
T4478	6690-6699	JJ	denotes	lysosomal
T4476	6700-6709	NN	denotes	targeting
T4473	6710-6716	NN	denotes	signal
T4480	6716-6717	.	denotes	.
T1674	6719-6729	NN	denotes	Expression
T1675	6730-6738	NN	denotes	analysis
T1676	6739-6741	IN	denotes	of
T1677	6742-6748	NN	denotes	Mcoln1
T1678	6748-7024	sentence	denotes	Mouse adult multiple tissue and embryonic Northern blots were hybridized using a probe generated from mouse exon 2 (probe 1, Fig. 1B), yielding a band of approximately 2.4 kb (isoform 1), as expected, and a less abundant and unexpected 4.4 kb band (isoform 2) (Figs. 3A &3B).
T1679	6749-6754	NN	denotes	Mouse
T1681	6755-6760	JJ	denotes	adult
T1682	6761-6769	JJ	denotes	multiple
T1680	6770-6776	NN	denotes	tissue
T1684	6777-6780	CC	denotes	and
T1685	6781-6790	JJ	denotes	embryonic
T1687	6791-6799	NNP	denotes	Northern
T1686	6800-6805	NNS	denotes	blots
T1688	6806-6810	VBD	denotes	were
T1683	6811-6821	VBN	denotes	hybridized
T1689	6822-6827	VBG	denotes	using
T1690	6828-6829	DT	denotes	a
T1691	6830-6835	NN	denotes	probe
T1692	6836-6845	VBN	denotes	generated
T1693	6846-6850	IN	denotes	from
T1694	6851-6856	NN	denotes	mouse
T1695	6857-6861	NN	denotes	exon
T1696	6862-6863	CD	denotes	2
T1697	6864-6865	-LRB-	denotes	(
T1699	6865-6870	NN	denotes	probe
T1700	6871-6872	CD	denotes	1
T1701	6872-6874	,	denotes	,
T1702	6874-6878	NN	denotes	Fig.
T1698	6879-6881	NN	denotes	1B
T1703	6881-6882	-RRB-	denotes	)
T1704	6882-6884	,	denotes	,
T1705	6884-6892	VBG	denotes	yielding
T1706	6893-6894	DT	denotes	a
T1707	6895-6899	NN	denotes	band
T1708	6900-6902	IN	denotes	of
T1709	6903-6916	RB	denotes	approximately
T1710	6917-6920	CD	denotes	2.4
T1711	6921-6923	NNS	denotes	kb
T1712	6924-6925	-LRB-	denotes	(
T1713	6925-6932	NN	denotes	isoform
T1714	6933-6934	CD	denotes	1
T1715	6934-6935	-RRB-	denotes	)
T1716	6935-6937	,	denotes	,
T1717	6937-6939	IN	denotes	as
T1718	6940-6948	VBN	denotes	expected
T1719	6948-6950	,	denotes	,
T1720	6950-6953	CC	denotes	and
T1721	6954-6955	DT	denotes	a
T1723	6956-6960	RBR	denotes	less
T1724	6961-6969	JJ	denotes	abundant
T1725	6970-6973	CC	denotes	and
T1726	6974-6984	JJ	denotes	unexpected
T1727	6985-6988	CD	denotes	4.4
T1728	6989-6991	NN	denotes	kb
T1722	6992-6996	NN	denotes	band
T1729	6997-6998	-LRB-	denotes	(
T1730	6998-7005	NN	denotes	isoform
T1731	7006-7007	CD	denotes	2
T1732	7007-7008	-RRB-	denotes	)
T1733	7009-7010	-LRB-	denotes	(
T1735	7010-7015	NNS	denotes	Figs.
T1734	7016-7018	NN	denotes	3A
T1736	7019-7020	CC	denotes	&
T1737	7020-7022	NN	denotes	3B
T1738	7022-7023	-RRB-	denotes	)
T1739	7023-7024	.	denotes	.
T1740	7024-7138	sentence	denotes	The 2.4 kb band shows ubiquitous but variable expression, with the highest expression in brain, liver and kidney.
T1741	7025-7028	DT	denotes	The
T1743	7029-7032	CD	denotes	2.4
T1744	7033-7035	NN	denotes	kb
T1742	7036-7040	NN	denotes	band
T1745	7041-7046	VBZ	denotes	shows
T1746	7047-7057	JJ	denotes	ubiquitous
T1748	7058-7061	CC	denotes	but
T1749	7062-7070	JJ	denotes	variable
T1747	7071-7081	NN	denotes	expression
T1750	7081-7083	,	denotes	,
T1751	7083-7087	IN	denotes	with
T1753	7088-7091	DT	denotes	the
T1754	7092-7099	JJS	denotes	highest
T1752	7100-7110	NN	denotes	expression
T1755	7111-7113	IN	denotes	in
T1756	7114-7119	NN	denotes	brain
T1757	7119-7121	,	denotes	,
T1758	7121-7126	NN	denotes	liver
T1759	7127-7130	CC	denotes	and
T1760	7131-7137	NN	denotes	kidney
T1761	7137-7138	.	denotes	.
T1762	7138-7239	sentence	denotes	The fetal tissue blot shows decreasing levels of the 2.4 kb message with increasing gestational age.
T1763	7139-7142	DT	denotes	The
T1765	7143-7148	JJ	denotes	fetal
T1766	7149-7155	NN	denotes	tissue
T1764	7156-7160	NN	denotes	blot
T1767	7161-7166	VBZ	denotes	shows
T1768	7167-7177	VBG	denotes	decreasing
T1769	7178-7184	NNS	denotes	levels
T1770	7185-7187	IN	denotes	of
T1771	7188-7191	DT	denotes	the
T1773	7192-7195	CD	denotes	2.4
T1774	7196-7198	NN	denotes	kb
T1772	7199-7206	NN	denotes	message
T1775	7207-7211	IN	denotes	with
T1776	7212-7222	VBG	denotes	increasing
T1778	7223-7234	JJ	denotes	gestational
T1777	7235-7238	NN	denotes	age
T1779	7238-7239	.	denotes	.
T1780	7239-7503	sentence	denotes	Since the human MCOLN1 gene encodes a single transcript [7-9], we carried out additional hybridizations with probes generated from various regions of the coding sequence and 3' UTR, and all probes identified the same two transcripts in the mouse (data not shown).
T1781	7240-7245	IN	denotes	Since
T1783	7246-7249	DT	denotes	the
T1785	7250-7255	JJ	denotes	human
T1786	7256-7262	NN	denotes	MCOLN1
T1784	7263-7267	NN	denotes	gene
T1782	7268-7275	VBZ	denotes	encodes
T1788	7276-7277	DT	denotes	a
T1790	7278-7284	JJ	denotes	single
T1789	7285-7295	NN	denotes	transcript
T1791	7296-7297	-LRB-	denotes	[
T1792	7297-7298	CD	denotes	7
T1793	7298-7299	SYM	denotes	-
T1794	7299-7300	CD	denotes	9
T1795	7300-7301	-RRB-	denotes	]
T1796	7301-7303	,	denotes	,
T1797	7303-7305	PRP	denotes	we
T1787	7306-7313	VBD	denotes	carried
T1798	7314-7317	RP	denotes	out
T1799	7318-7328	JJ	denotes	additional
T1800	7329-7343	NNS	denotes	hybridizations
T1801	7344-7348	IN	denotes	with
T1802	7349-7355	NNS	denotes	probes
T1803	7356-7365	VBN	denotes	generated
T1804	7366-7370	IN	denotes	from
T1805	7371-7378	JJ	denotes	various
T1806	7379-7386	NNS	denotes	regions
T1807	7387-7389	IN	denotes	of
T1808	7390-7393	DT	denotes	the
T1810	7394-7400	VBG	denotes	coding
T1809	7401-7409	NN	denotes	sequence
T1811	7410-7413	CC	denotes	and
T1812	7414-7415	CD	denotes	3
T1814	7415-7416	SYM	denotes	'
T1813	7417-7420	NN	denotes	UTR
T1815	7420-7422	,	denotes	,
T1816	7422-7425	CC	denotes	and
T1817	7426-7429	DT	denotes	all
T1818	7430-7436	NNS	denotes	probes
T1819	7437-7447	VBD	denotes	identified
T1820	7448-7451	DT	denotes	the
T1822	7452-7456	JJ	denotes	same
T1823	7457-7460	CD	denotes	two
T1821	7461-7472	NNS	denotes	transcripts
T1824	7473-7475	IN	denotes	in
T1825	7476-7479	DT	denotes	the
T1826	7480-7485	NN	denotes	mouse
T1827	7486-7487	-LRB-	denotes	(
T1829	7487-7491	NNS	denotes	data
T1830	7492-7495	RB	denotes	not
T1828	7496-7501	VBN	denotes	shown
T1831	7501-7502	-RRB-	denotes	)
T1832	7502-7503	.	denotes	.
T1833	7503-7619	sentence	denotes	In order to verify the presence of a single mouse locus, we hybridized a mouse Southern blot with the exon 2 probe.
T1834	7504-7506	IN	denotes	In
T1836	7507-7512	NN	denotes	order
T1837	7513-7515	TO	denotes	to
T1838	7516-7522	VB	denotes	verify
T1839	7523-7526	DT	denotes	the
T1840	7527-7535	NN	denotes	presence
T1841	7536-7538	IN	denotes	of
T1842	7539-7540	DT	denotes	a
T1844	7541-7547	JJ	denotes	single
T1845	7548-7553	NN	denotes	mouse
T1843	7554-7559	NN	denotes	locus
T1846	7559-7561	,	denotes	,
T1847	7561-7563	PRP	denotes	we
T1835	7564-7574	VBD	denotes	hybridized
T1848	7575-7576	DT	denotes	a
T1850	7577-7582	NN	denotes	mouse
T1851	7583-7591	NN	denotes	Southern
T1849	7592-7596	NN	denotes	blot
T1852	7597-7601	IN	denotes	with
T1853	7602-7605	DT	denotes	the
T1855	7606-7610	NN	denotes	exon
T1856	7611-7612	CD	denotes	2
T1854	7613-7618	NN	denotes	probe
T1857	7618-7619	.	denotes	.
T1858	7619-7757	sentence	denotes	Four different restriction enzymes were used, and only the expected size bands for the chromosome 8 locus were detected (data not shown).
T1859	7620-7624	CD	denotes	Four
T1861	7625-7634	JJ	denotes	different
T1862	7635-7646	NN	denotes	restriction
T1860	7647-7654	NNS	denotes	enzymes
T1864	7655-7659	VBD	denotes	were
T1863	7660-7664	VBN	denotes	used
T1865	7664-7666	,	denotes	,
T1866	7666-7669	CC	denotes	and
T1867	7670-7674	RB	denotes	only
T1869	7675-7678	DT	denotes	the
T1870	7679-7687	VBN	denotes	expected
T1871	7688-7692	NN	denotes	size
T1868	7693-7698	NNS	denotes	bands
T1873	7699-7702	IN	denotes	for
T1874	7703-7706	DT	denotes	the
T1876	7707-7717	NN	denotes	chromosome
T1877	7718-7719	CD	denotes	8
T1875	7720-7725	NN	denotes	locus
T1878	7726-7730	VBD	denotes	were
T1872	7731-7739	VBN	denotes	detected
T1879	7740-7741	-LRB-	denotes	(
T1881	7741-7745	NNS	denotes	data
T1882	7746-7749	RB	denotes	not
T1880	7750-7755	VBN	denotes	shown
T1883	7755-7756	-RRB-	denotes	)
T1884	7756-7757	.	denotes	.
T4522	7768-7776	NNP	denotes	Northern
T4523	7777-7785	NN	denotes	analysis
T4524	7786-7788	IN	denotes	of
T4525	7789-7795	NN	denotes	Mcoln1
T4526	7795-7796	.	denotes	.
T4527	7796-7950	sentence	denotes	(A) Mouse fetal tissue Northern and (B) mouse multiple tissue Northern blots (Clontech) hybridized with a probe generated from exon 2 (probe 1, Fig. 1B).
T4528	7797-7798	-LRB-	denotes	(
T4529	7798-7799	LS	denotes	A
T4531	7799-7800	-RRB-	denotes	)
T4532	7801-7806	NN	denotes	Mouse
T4534	7807-7812	JJ	denotes	fetal
T4533	7813-7819	NN	denotes	tissue
T4530	7820-7828	NN	denotes	Northern
T4536	7829-7832	CC	denotes	and
T4537	7833-7834	-LRB-	denotes	(
T4538	7834-7835	LS	denotes	B
T4540	7835-7836	-RRB-	denotes	)
T4541	7837-7842	NN	denotes	mouse
T4543	7843-7851	JJ	denotes	multiple
T4542	7852-7858	NN	denotes	tissue
T4539	7859-7867	NN	denotes	Northern
T4535	7868-7873	NNS	denotes	blots
T4545	7874-7875	-LRB-	denotes	(
T4546	7875-7883	NN	denotes	Clontech
T4547	7883-7884	-RRB-	denotes	)
T4544	7885-7895	VBD	denotes	hybridized
T4548	7896-7900	IN	denotes	with
T4549	7901-7902	DT	denotes	a
T4550	7903-7908	NN	denotes	probe
T4551	7909-7918	VBN	denotes	generated
T4552	7919-7923	IN	denotes	from
T4553	7924-7928	NN	denotes	exon
T4554	7929-7930	CD	denotes	2
T4555	7931-7932	-LRB-	denotes	(
T4557	7932-7937	NN	denotes	probe
T4558	7938-7939	CD	denotes	1
T4559	7939-7941	,	denotes	,
T4560	7941-7945	NN	denotes	Fig.
T4556	7946-7948	NN	denotes	1B
T4561	7948-7949	-RRB-	denotes	)
T4562	7949-7950	.	denotes	.
T4563	7950-8079	sentence	denotes	(C) Hybridization of the multiple tissue blot with a probe from the alternatively spliced segment of exon 13 (probe 2, Fig. 1B).
T4564	7951-7952	-LRB-	denotes	(
T4565	7952-7953	LS	denotes	C
T4567	7953-7954	-RRB-	denotes	)
T4566	7955-7968	NN	denotes	Hybridization
T4568	7969-7971	IN	denotes	of
T4569	7972-7975	DT	denotes	the
T4571	7976-7984	JJ	denotes	multiple
T4572	7985-7991	NN	denotes	tissue
T4570	7992-7996	NN	denotes	blot
T4573	7997-8001	IN	denotes	with
T4574	8002-8003	DT	denotes	a
T4575	8004-8009	NN	denotes	probe
T4576	8010-8014	IN	denotes	from
T4577	8015-8018	DT	denotes	the
T4579	8019-8032	RB	denotes	alternatively
T4580	8033-8040	VBN	denotes	spliced
T4578	8041-8048	NN	denotes	segment
T4581	8049-8051	IN	denotes	of
T4582	8052-8056	NN	denotes	exon
T4583	8057-8059	CD	denotes	13
T4584	8060-8061	-LRB-	denotes	(
T4586	8061-8066	NN	denotes	probe
T4587	8067-8068	CD	denotes	2
T4588	8068-8070	,	denotes	,
T4589	8070-8074	NN	denotes	Fig.
T4585	8075-8077	NN	denotes	1B
T4590	8077-8078	-RRB-	denotes	)
T4591	8078-8079	.	denotes	.
T4592	8079-8135	sentence	denotes	β-Actin control hybridization is shown below each blot.
T4593	8080-8081	NN	denotes	β
T4595	8081-8082	HYPH	denotes	-
T4594	8082-8087	NN	denotes	Actin
T4597	8088-8095	NN	denotes	control
T4596	8096-8109	NN	denotes	hybridization
T4599	8110-8112	VBZ	denotes	is
T4598	8113-8118	VBN	denotes	shown
T4600	8119-8124	IN	denotes	below
T4601	8125-8129	DT	denotes	each
T4602	8130-8134	NN	denotes	blot
T4603	8134-8135	.	denotes	.
T2105	8137-8153	NN	denotes	Characterization
T2106	8154-8156	IN	denotes	of
T2107	8157-8160	DT	denotes	the
T2109	8161-8167	NN	denotes	Mcoln1
T2110	8168-8179	JJ	denotes	alternative
T2111	8180-8186	NN	denotes	splice
T2108	8187-8194	NN	denotes	variant
T2112	8194-8374	sentence	denotes	In order to determine the coding sequence for the larger transcript, we searched the mouse EST database using each intron as well as the genomic sequence flanking the Mcoln1 gene.
T2113	8195-8197	IN	denotes	In
T2115	8198-8203	NN	denotes	order
T2116	8204-8206	TO	denotes	to
T2117	8207-8216	VB	denotes	determine
T2118	8217-8220	DT	denotes	the
T2120	8221-8227	VBG	denotes	coding
T2119	8228-8236	NN	denotes	sequence
T2121	8237-8240	IN	denotes	for
T2122	8241-8244	DT	denotes	the
T2124	8245-8251	JJR	denotes	larger
T2123	8252-8262	NN	denotes	transcript
T2125	8262-8264	,	denotes	,
T2126	8264-8266	PRP	denotes	we
T2114	8267-8275	VBD	denotes	searched
T2127	8276-8279	DT	denotes	the
T2129	8280-8285	NN	denotes	mouse
T2130	8286-8289	NN	denotes	EST
T2128	8290-8298	NN	denotes	database
T2131	8299-8304	VBG	denotes	using
T2132	8305-8309	DT	denotes	each
T2133	8310-8316	NN	denotes	intron
T2134	8317-8319	RB	denotes	as
T2136	8320-8324	RB	denotes	well
T2135	8325-8327	IN	denotes	as
T2137	8328-8331	DT	denotes	the
T2139	8332-8339	JJ	denotes	genomic
T2138	8340-8348	NN	denotes	sequence
T2140	8349-8357	VBG	denotes	flanking
T2141	8358-8361	DT	denotes	the
T2143	8362-8368	NN	denotes	Mcoln1
T2142	8369-8373	NN	denotes	gene
T2144	8373-8374	.	denotes	.
T2145	8374-8515	sentence	denotes	Two ESTs were identified that contained sequence from intron 12 (GB No. AI430291 and AA874645), and the corresponding clones were sequenced.
T2146	8375-8378	CD	denotes	Two
T2147	8379-8383	NNS	denotes	ESTs
T2149	8384-8388	VBD	denotes	were
T2148	8389-8399	VBN	denotes	identified
T2150	8400-8404	WDT	denotes	that
T2151	8405-8414	VBD	denotes	contained
T2152	8415-8423	NN	denotes	sequence
T2153	8424-8428	IN	denotes	from
T2154	8429-8435	NN	denotes	intron
T2155	8436-8438	CD	denotes	12
T2156	8439-8440	-LRB-	denotes	(
T2158	8440-8442	NN	denotes	GB
T2159	8443-8446	NN	denotes	No.
T2157	8447-8455	NN	denotes	AI430291
T2160	8456-8459	CC	denotes	and
T2161	8460-8468	NN	denotes	AA874645
T2162	8468-8469	-RRB-	denotes	)
T2163	8469-8471	,	denotes	,
T2164	8471-8474	CC	denotes	and
T2165	8475-8478	DT	denotes	the
T2167	8479-8492	JJ	denotes	corresponding
T2166	8493-8499	NNS	denotes	clones
T2169	8500-8504	VBD	denotes	were
T2168	8505-8514	VBN	denotes	sequenced
T2170	8514-8515	.	denotes	.
T2171	8515-8670	sentence	denotes	Clone 408619 (ESTs: GB No. AI430291, AI429558) begins approximately 1.1 kb before exon 13 and continues through the exon and splices correctly to exon 14.
T2172	8516-8521	NN	denotes	Clone
T2174	8522-8528	CD	denotes	408619
T2175	8529-8530	-LRB-	denotes	(
T2177	8530-8534	NNS	denotes	ESTs
T2178	8534-8536	:	denotes	:
T2179	8536-8538	NN	denotes	GB
T2180	8539-8542	NN	denotes	No.
T2181	8543-8551	NN	denotes	AI430291
T2182	8551-8553	,	denotes	,
T2176	8553-8561	NN	denotes	AI429558
T2183	8561-8562	-RRB-	denotes	)
T2173	8563-8569	VBZ	denotes	begins
T2184	8570-8583	RB	denotes	approximately
T2185	8584-8587	CD	denotes	1.1
T2186	8588-8590	NNS	denotes	kb
T2187	8591-8597	IN	denotes	before
T2188	8598-8602	NN	denotes	exon
T2189	8603-8605	CD	denotes	13
T2190	8606-8609	CC	denotes	and
T2191	8610-8619	VBZ	denotes	continues
T2192	8620-8627	IN	denotes	through
T2193	8628-8631	DT	denotes	the
T2194	8632-8636	NN	denotes	exon
T2195	8637-8640	CC	denotes	and
T2196	8641-8648	VBZ	denotes	splices
T2197	8649-8658	RB	denotes	correctly
T2198	8659-8661	IN	denotes	to
T2199	8662-8666	NN	denotes	exon
T2200	8667-8669	CD	denotes	14
T2201	8669-8670	.	denotes	.
T2202	8670-8774	sentence	denotes	Clone 1281641 (EST:GB No. AA874645) begins 175 bp before exon 13 and also splices correctly to exon 14.
T2203	8671-8676	NN	denotes	Clone
T2205	8677-8684	CD	denotes	1281641
T2206	8685-8686	-LRB-	denotes	(
T2208	8686-8689	NN	denotes	EST
T2209	8689-8690	:	denotes	:
T2210	8690-8692	NN	denotes	GB
T2211	8693-8696	NN	denotes	No.
T2207	8697-8705	NN	denotes	AA874645
T2212	8705-8706	-RRB-	denotes	)
T2204	8707-8713	VBZ	denotes	begins
T2213	8714-8717	CD	denotes	175
T2214	8718-8720	NNS	denotes	bp
T2215	8721-8727	IN	denotes	before
T2216	8728-8732	NN	denotes	exon
T2217	8733-8735	CD	denotes	13
T2218	8736-8739	CC	denotes	and
T2219	8740-8744	RB	denotes	also
T2220	8745-8752	VBZ	denotes	splices
T2221	8753-8762	RB	denotes	correctly
T2222	8763-8765	IN	denotes	to
T2223	8766-8770	NN	denotes	exon
T2224	8771-8773	CD	denotes	14
T2225	8773-8774	.	denotes	.
T2226	8774-8963	sentence	denotes	A mouse multiple tissue Northern was hybridized using a probe generated from the putative intron sequence in clone 408619 (Probe 2, Fig. 1B), which detected only the 4.4 kb band (Fig. 3C).
T2227	8775-8776	DT	denotes	A
T2229	8777-8782	NN	denotes	mouse
T2230	8783-8791	JJ	denotes	multiple
T2231	8792-8798	NN	denotes	tissue
T2228	8799-8807	NNP	denotes	Northern
T2233	8808-8811	VBD	denotes	was
T2232	8812-8822	VBN	denotes	hybridized
T2234	8823-8828	VBG	denotes	using
T2235	8829-8830	DT	denotes	a
T2236	8831-8836	NN	denotes	probe
T2237	8837-8846	VBN	denotes	generated
T2238	8847-8851	IN	denotes	from
T2239	8852-8855	DT	denotes	the
T2241	8856-8864	JJ	denotes	putative
T2242	8865-8871	NN	denotes	intron
T2240	8872-8880	NN	denotes	sequence
T2243	8881-8883	IN	denotes	in
T2244	8884-8889	NN	denotes	clone
T2245	8890-8896	CD	denotes	408619
T2246	8897-8898	-LRB-	denotes	(
T2248	8898-8903	NN	denotes	Probe
T2249	8904-8905	CD	denotes	2
T2250	8905-8907	,	denotes	,
T2251	8907-8911	NNP	denotes	Fig.
T2247	8912-8914	NN	denotes	1B
T2252	8914-8915	-RRB-	denotes	)
T2253	8915-8917	,	denotes	,
T2254	8917-8922	WDT	denotes	which
T2255	8923-8931	VBD	denotes	detected
T2256	8932-8936	RB	denotes	only
T2258	8937-8940	DT	denotes	the
T2259	8941-8944	CD	denotes	4.4
T2260	8945-8947	NN	denotes	kb
T2257	8948-8952	NN	denotes	band
T2261	8953-8954	-LRB-	denotes	(
T2263	8954-8958	NN	denotes	Fig.
T2262	8959-8961	NN	denotes	3C
T2264	8961-8962	-RRB-	denotes	)
T2265	8962-8963	.	denotes	.
T2266	8963-9185	sentence	denotes	In order to determine the sequence of the entire transcript, RT-PCR using primers in exons 10 and 11 paired with a primer in intron 12 was performed using BALB/c mouse brain total RNA and the resulting products sequenced.
T2267	8964-8966	IN	denotes	In
T2269	8967-8972	NN	denotes	order
T2270	8973-8975	TO	denotes	to
T2271	8976-8985	VB	denotes	determine
T2272	8986-8989	DT	denotes	the
T2273	8990-8998	NN	denotes	sequence
T2274	8999-9001	IN	denotes	of
T2275	9002-9005	DT	denotes	the
T2277	9006-9012	JJ	denotes	entire
T2276	9013-9023	NN	denotes	transcript
T2278	9023-9025	,	denotes	,
T2279	9025-9027	NN	denotes	RT
T2281	9027-9028	HYPH	denotes	-
T2280	9028-9031	NN	denotes	PCR
T2282	9032-9037	VBG	denotes	using
T2283	9038-9045	NNS	denotes	primers
T2284	9046-9048	IN	denotes	in
T2285	9049-9054	NNS	denotes	exons
T2286	9055-9057	CD	denotes	10
T2287	9058-9061	CC	denotes	and
T2288	9062-9064	CD	denotes	11
T2289	9065-9071	VBN	denotes	paired
T2290	9072-9076	IN	denotes	with
T2291	9077-9078	DT	denotes	a
T2292	9079-9085	NN	denotes	primer
T2293	9086-9088	IN	denotes	in
T2294	9089-9095	NN	denotes	intron
T2295	9096-9098	CD	denotes	12
T2296	9099-9102	VBD	denotes	was
T2268	9103-9112	VBN	denotes	performed
T2297	9113-9118	VBG	denotes	using
T2298	9119-9123	NN	denotes	BALB
T2300	9123-9124	HYPH	denotes	/
T2299	9124-9125	NN	denotes	c
T2301	9126-9131	NN	denotes	mouse
T2302	9132-9137	NN	denotes	brain
T2304	9138-9143	JJ	denotes	total
T2303	9144-9147	NN	denotes	RNA
T2305	9148-9151	CC	denotes	and
T2306	9152-9155	DT	denotes	the
T2308	9156-9165	VBG	denotes	resulting
T2307	9166-9174	NNS	denotes	products
T2309	9175-9184	VBN	denotes	sequenced
T2310	9184-9185	.	denotes	.
T2311	9185-9307	sentence	denotes	These products show that the larger transcript is due to an alternative splice event that results in an expanded exon 13.
T2312	9186-9191	DT	denotes	These
T2313	9192-9200	NNS	denotes	products
T2314	9201-9205	VBP	denotes	show
T2315	9206-9210	IN	denotes	that
T2317	9211-9214	DT	denotes	the
T2319	9215-9221	JJR	denotes	larger
T2318	9222-9232	NN	denotes	transcript
T2316	9233-9235	VBZ	denotes	is
T2320	9236-9239	IN	denotes	due
T2321	9240-9242	IN	denotes	to
T2322	9243-9245	DT	denotes	an
T2324	9246-9257	JJ	denotes	alternative
T2325	9258-9264	NN	denotes	splice
T2323	9265-9270	NN	denotes	event
T2326	9271-9275	WDT	denotes	that
T2327	9276-9283	VBZ	denotes	results
T2328	9284-9286	IN	denotes	in
T2329	9287-9289	DT	denotes	an
T2331	9290-9298	VBN	denotes	expanded
T2330	9299-9303	NN	denotes	exon
T2332	9304-9306	CD	denotes	13
T2333	9306-9307	.	denotes	.
T2334	9307-9429	sentence	denotes	Specifically, exon 12 splices at bp 436 of intron 12, creating a large 1614 bp exon 13 that splices correctly to exon 14.
T2335	9308-9320	RB	denotes	Specifically
T2337	9320-9322	,	denotes	,
T2338	9322-9326	NN	denotes	exon
T2339	9327-9329	CD	denotes	12
T2336	9330-9337	VBZ	denotes	splices
T2340	9338-9340	IN	denotes	at
T2341	9341-9343	NN	denotes	bp
T2342	9344-9347	CD	denotes	436
T2343	9348-9350	IN	denotes	of
T2344	9351-9357	NN	denotes	intron
T2345	9358-9360	CD	denotes	12
T2346	9360-9362	,	denotes	,
T2347	9362-9370	VBG	denotes	creating
T2348	9371-9372	DT	denotes	a
T2350	9373-9378	JJ	denotes	large
T2351	9379-9383	CD	denotes	1614
T2352	9384-9386	NN	denotes	bp
T2349	9387-9391	NN	denotes	exon
T2353	9392-9394	CD	denotes	13
T2354	9395-9399	WDT	denotes	that
T2355	9400-9407	VBZ	denotes	splices
T2356	9408-9417	RB	denotes	correctly
T2357	9418-9420	IN	denotes	to
T2358	9421-9425	NN	denotes	exon
T2359	9426-9428	CD	denotes	14
T2360	9428-9429	.	denotes	.
T2361	9429-9586	sentence	denotes	The open reading frame of this alternatively spliced transcript is 611 amino acids, 28 amino acids longer than the message encoded by the 2.4 kb transcript.
T2362	9430-9433	DT	denotes	The
T2364	9434-9438	JJ	denotes	open
T2365	9439-9446	NN	denotes	reading
T2363	9447-9452	NN	denotes	frame
T2367	9453-9455	IN	denotes	of
T2368	9456-9460	DT	denotes	this
T2370	9461-9474	RB	denotes	alternatively
T2371	9475-9482	VBN	denotes	spliced
T2369	9483-9493	NN	denotes	transcript
T2366	9494-9496	VBZ	denotes	is
T2372	9497-9500	CD	denotes	611
T2374	9501-9506	NN	denotes	amino
T2373	9507-9512	NNS	denotes	acids
T2375	9512-9514	,	denotes	,
T2376	9514-9516	CD	denotes	28
T2378	9517-9522	JJ	denotes	amino
T2377	9523-9528	NNS	denotes	acids
T2379	9529-9535	JJR	denotes	longer
T2380	9536-9540	IN	denotes	than
T2381	9541-9544	DT	denotes	the
T2382	9545-9552	NN	denotes	message
T2383	9553-9560	VBN	denotes	encoded
T2384	9561-9563	IN	denotes	by
T2385	9564-9567	DT	denotes	the
T2387	9568-9571	CD	denotes	2.4
T2388	9572-9574	NN	denotes	kb
T2386	9575-9585	NN	denotes	transcript
T2389	9585-9586	.	denotes	.
T2390	9586-9790	sentence	denotes	TMPred analysis predicts that isoform 2 encodes a protein identical in structure to Mcoln1, possessing 6 transmembrane domains and a channel pore, however the protein sequences diverge at amino acid 526.
T2391	9587-9593	VBN	denotes	TMPred
T2392	9594-9602	NN	denotes	analysis
T2393	9603-9611	VBZ	denotes	predicts
T2395	9612-9616	IN	denotes	that
T2397	9617-9624	NN	denotes	isoform
T2398	9625-9626	CD	denotes	2
T2396	9627-9634	VBZ	denotes	encodes
T2399	9635-9636	DT	denotes	a
T2400	9637-9644	NN	denotes	protein
T2401	9645-9654	JJ	denotes	identical
T2402	9655-9657	IN	denotes	in
T2403	9658-9667	NN	denotes	structure
T2404	9668-9670	IN	denotes	to
T2405	9671-9677	NN	denotes	Mcoln1
T2406	9677-9679	,	denotes	,
T2407	9679-9689	VBG	denotes	possessing
T2408	9690-9691	CD	denotes	6
T2410	9692-9705	NN	denotes	transmembrane
T2409	9706-9713	NNS	denotes	domains
T2411	9714-9717	CC	denotes	and
T2412	9718-9719	DT	denotes	a
T2414	9720-9727	NN	denotes	channel
T2413	9728-9732	NN	denotes	pore
T2415	9732-9734	,	denotes	,
T2416	9734-9741	RB	denotes	however
T2417	9742-9745	DT	denotes	the
T2419	9746-9753	NN	denotes	protein
T2418	9754-9763	NNS	denotes	sequences
T2394	9764-9771	VBP	denotes	diverge
T2420	9772-9774	IN	denotes	at
T2421	9775-9780	JJ	denotes	amino
T2422	9781-9785	NN	denotes	acid
T2423	9786-9789	CD	denotes	526
T2424	9789-9790	.	denotes	.
T2425	9790-9980	sentence	denotes	The 55 amino acid C-terminal cytoplasmic tail encoded by the 2.4 kb transcript is completely different from the 86 amino acid tail encoded by the murine specific 4.4 kb transcript (Fig. 4).
T2426	9791-9794	DT	denotes	The
T2428	9795-9797	CD	denotes	55
T2430	9798-9803	NN	denotes	amino
T2429	9804-9808	NN	denotes	acid
T2431	9809-9810	NN	denotes	C
T2433	9810-9811	HYPH	denotes	-
T2432	9811-9819	JJ	denotes	terminal
T2434	9820-9831	JJ	denotes	cytoplasmic
T2427	9832-9836	NN	denotes	tail
T2436	9837-9844	VBN	denotes	encoded
T2437	9845-9847	IN	denotes	by
T2438	9848-9851	DT	denotes	the
T2440	9852-9855	CD	denotes	2.4
T2441	9856-9858	NN	denotes	kb
T2439	9859-9869	NN	denotes	transcript
T2435	9870-9872	VBZ	denotes	is
T2442	9873-9883	RB	denotes	completely
T2443	9884-9893	JJ	denotes	different
T2444	9894-9898	IN	denotes	from
T2445	9899-9902	DT	denotes	the
T2447	9903-9905	CD	denotes	86
T2449	9906-9911	NN	denotes	amino
T2448	9912-9916	NN	denotes	acid
T2446	9917-9921	NN	denotes	tail
T2450	9922-9929	VBN	denotes	encoded
T2451	9930-9932	IN	denotes	by
T2452	9933-9936	DT	denotes	the
T2454	9937-9943	NN	denotes	murine
T2455	9944-9952	JJ	denotes	specific
T2456	9953-9956	CD	denotes	4.4
T2457	9957-9959	NN	denotes	kb
T2453	9960-9970	NN	denotes	transcript
T2458	9971-9972	-LRB-	denotes	(
T2459	9972-9976	NN	denotes	Fig.
T2460	9977-9978	CD	denotes	4
T2461	9978-9979	-RRB-	denotes	)
T2462	9979-9980	.	denotes	.
T2463	9980-10269	sentence	denotes	Clontech Mouse RNA Master Blots were hybridized with the exon 2 and intron 12 probes mentioned above in an attempt to determine if these two transcripts showed differences in expression patterns, however, there was no significant difference in the 22 tissues represented (data not shown).
T2464	9981-9989	NNP	denotes	Clontech
T2466	9990-9995	NNP	denotes	Mouse
T2467	9996-9999	NNP	denotes	RNA
T2468	10000-10006	NNP	denotes	Master
T2465	10007-10012	NNPS	denotes	Blots
T2470	10013-10017	VBD	denotes	were
T2469	10018-10028	VBN	denotes	hybridized
T2472	10029-10033	IN	denotes	with
T2473	10034-10037	DT	denotes	the
T2475	10038-10042	NN	denotes	exon
T2476	10043-10044	CD	denotes	2
T2477	10045-10048	CC	denotes	and
T2478	10049-10055	NN	denotes	intron
T2479	10056-10058	CD	denotes	12
T2474	10059-10065	NNS	denotes	probes
T2480	10066-10075	VBN	denotes	mentioned
T2481	10076-10081	RB	denotes	above
T2482	10082-10084	IN	denotes	in
T2483	10085-10087	DT	denotes	an
T2484	10088-10095	NN	denotes	attempt
T2485	10096-10098	TO	denotes	to
T2486	10099-10108	VB	denotes	determine
T2487	10109-10111	IN	denotes	if
T2489	10112-10117	DT	denotes	these
T2491	10118-10121	CD	denotes	two
T2490	10122-10133	NNS	denotes	transcripts
T2488	10134-10140	VBD	denotes	showed
T2492	10141-10152	NNS	denotes	differences
T2493	10153-10155	IN	denotes	in
T2494	10156-10166	NN	denotes	expression
T2495	10167-10175	NNS	denotes	patterns
T2496	10175-10177	,	denotes	,
T2497	10177-10184	RB	denotes	however
T2498	10184-10186	,	denotes	,
T2499	10186-10191	EX	denotes	there
T2471	10192-10195	VBD	denotes	was
T2500	10196-10198	DT	denotes	no
T2502	10199-10210	JJ	denotes	significant
T2501	10211-10221	NN	denotes	difference
T2503	10222-10224	IN	denotes	in
T2504	10225-10228	DT	denotes	the
T2506	10229-10231	CD	denotes	22
T2505	10232-10239	NNS	denotes	tissues
T2507	10240-10251	VBN	denotes	represented
T2508	10252-10253	-LRB-	denotes	(
T2510	10253-10257	NNS	denotes	data
T2511	10258-10261	RB	denotes	not
T2509	10262-10267	VBN	denotes	shown
T2512	10267-10268	-RRB-	denotes	)
T2513	10268-10269	.	denotes	.
T2514	10269-10669	sentence	denotes	Figure 4 Peptide sequence comparison of the two alternatively spliced Mcoln1 isoforms. The green box surrounds the divergent c-terminal cytoplasmic tails. The blue lines indicate the transmembrane domains. Next, we directly compared the nucleotide and amino acid sequence of the alternatively spliced mouse transcript to the entire human MCOLN1 genomic sequence and found no significant similarity.
T4631	10280-10287	NN	denotes	Peptide
T4632	10288-10296	NN	denotes	sequence
T4633	10297-10307	NN	denotes	comparison
T4634	10308-10310	IN	denotes	of
T4635	10311-10314	DT	denotes	the
T4637	10315-10318	CD	denotes	two
T4638	10319-10332	RB	denotes	alternatively
T4639	10333-10340	VBN	denotes	spliced
T4640	10341-10347	NN	denotes	Mcoln1
T4636	10348-10356	NNS	denotes	isoforms
T4641	10356-10357	.	denotes	.
T4642	10357-10425	sentence	denotes	The green box surrounds the divergent c-terminal cytoplasmic tails.
T4643	10358-10361	DT	denotes	The
T4645	10362-10367	JJ	denotes	green
T4644	10368-10371	NN	denotes	box
T4646	10372-10381	VBZ	denotes	surrounds
T4647	10382-10385	DT	denotes	the
T4649	10386-10395	JJ	denotes	divergent
T4650	10396-10397	NN	denotes	c
T4652	10397-10398	HYPH	denotes	-
T4651	10398-10406	JJ	denotes	terminal
T4653	10407-10418	JJ	denotes	cytoplasmic
T4648	10419-10424	NNS	denotes	tails
T4654	10424-10425	.	denotes	.
T4655	10425-10476	sentence	denotes	The blue lines indicate the transmembrane domains.
T4656	10426-10429	DT	denotes	The
T4658	10430-10434	JJ	denotes	blue
T4657	10435-10440	NNS	denotes	lines
T4659	10441-10449	VBP	denotes	indicate
T4660	10450-10453	DT	denotes	the
T4662	10454-10467	NN	denotes	transmembrane
T4661	10468-10475	NNS	denotes	domains
T4663	10475-10476	.	denotes	.
T2515	10477-10481	RB	denotes	Next
T2517	10481-10483	,	denotes	,
T2518	10483-10485	PRP	denotes	we
T2519	10486-10494	RB	denotes	directly
T2516	10495-10503	VBD	denotes	compared
T2520	10504-10507	DT	denotes	the
T2522	10508-10518	NN	denotes	nucleotide
T2523	10519-10522	CC	denotes	and
T2524	10523-10528	NN	denotes	amino
T2525	10529-10533	NN	denotes	acid
T2521	10534-10542	NN	denotes	sequence
T2526	10543-10545	IN	denotes	of
T2527	10546-10549	DT	denotes	the
T2529	10550-10563	RB	denotes	alternatively
T2530	10564-10571	VBN	denotes	spliced
T2531	10572-10577	NN	denotes	mouse
T2528	10578-10588	NN	denotes	transcript
T2532	10589-10591	IN	denotes	to
T2533	10592-10595	DT	denotes	the
T2535	10596-10602	JJ	denotes	entire
T2536	10603-10608	JJ	denotes	human
T2537	10609-10615	NN	denotes	MCOLN1
T2538	10616-10623	JJ	denotes	genomic
T2534	10624-10632	NN	denotes	sequence
T2539	10633-10636	CC	denotes	and
T2540	10637-10642	VBD	denotes	found
T2541	10643-10645	DT	denotes	no
T2543	10646-10657	JJ	denotes	significant
T2542	10658-10668	NN	denotes	similarity
T2544	10668-10669	.	denotes	.
T2545	10669-10777	sentence	denotes	As mentioned previously, Northern blots performed with human MCOLN1 probes show only one 2.4 kb transcript.
T2546	10670-10672	IN	denotes	As
T2547	10673-10682	VBN	denotes	mentioned
T2549	10683-10693	RB	denotes	previously
T2550	10693-10695	,	denotes	,
T2551	10695-10703	NNP	denotes	Northern
T2552	10704-10709	NNS	denotes	blots
T2553	10710-10719	VBN	denotes	performed
T2554	10720-10724	IN	denotes	with
T2555	10725-10730	JJ	denotes	human
T2556	10731-10737	NN	denotes	MCOLN1
T2557	10738-10744	NNS	denotes	probes
T2548	10745-10749	VBP	denotes	show
T2558	10750-10754	RB	denotes	only
T2560	10755-10758	CD	denotes	one
T2561	10759-10762	CD	denotes	2.4
T2562	10763-10765	NN	denotes	kb
T2559	10766-10776	NN	denotes	transcript
T2563	10776-10777	.	denotes	.
T2564	10777-10917	sentence	denotes	In addition, we hybridized a human multiple tissue Northern and human Southern with a probe in human intron 12 that is adjacent to exon 13.
T2565	10778-10780	IN	denotes	In
T2567	10781-10789	NN	denotes	addition
T2568	10789-10791	,	denotes	,
T2569	10791-10793	PRP	denotes	we
T2566	10794-10804	VBD	denotes	hybridized
T2570	10805-10806	DT	denotes	a
T2572	10807-10812	JJ	denotes	human
T2573	10813-10821	JJ	denotes	multiple
T2574	10822-10828	NN	denotes	tissue
T2571	10829-10837	NNP	denotes	Northern
T2575	10838-10841	CC	denotes	and
T2576	10842-10847	JJ	denotes	human
T2577	10848-10856	NNP	denotes	Southern
T2578	10857-10861	IN	denotes	with
T2579	10862-10863	DT	denotes	a
T2580	10864-10869	NN	denotes	probe
T2581	10870-10872	IN	denotes	in
T2582	10873-10878	JJ	denotes	human
T2583	10879-10885	NN	denotes	intron
T2584	10886-10888	CD	denotes	12
T2585	10889-10893	WDT	denotes	that
T2586	10894-10896	VBZ	denotes	is
T2587	10897-10905	JJ	denotes	adjacent
T2588	10906-10908	IN	denotes	to
T2589	10909-10913	NN	denotes	exon
T2590	10914-10916	CD	denotes	13
T2591	10916-10917	.	denotes	.
T2592	10917-11016	sentence	denotes	The probe was located in the region syntenic to that which encodes the alternate mouse transcript.
T2593	10918-10921	DT	denotes	The
T2594	10922-10927	NN	denotes	probe
T2596	10928-10931	VBD	denotes	was
T2595	10932-10939	VBN	denotes	located
T2597	10940-10942	IN	denotes	in
T2598	10943-10946	DT	denotes	the
T2599	10947-10953	NN	denotes	region
T2600	10954-10962	JJ	denotes	syntenic
T2601	10963-10965	IN	denotes	to
T2602	10966-10970	DT	denotes	that
T2603	10971-10976	WDT	denotes	which
T2604	10977-10984	VBZ	denotes	encodes
T2605	10985-10988	DT	denotes	the
T2607	10989-10998	JJ	denotes	alternate
T2608	10999-11004	NN	denotes	mouse
T2606	11005-11015	NN	denotes	transcript
T2609	11015-11016	.	denotes	.
T2610	11016-11188	sentence	denotes	Only the expected bands were detected on the Southern and no bands were detected on the Northern, confirming that this alternative transcript is specific to murine Mcoln1.
T2611	11017-11021	RB	denotes	Only
T2613	11022-11025	DT	denotes	the
T2614	11026-11034	VBN	denotes	expected
T2612	11035-11040	NNS	denotes	bands
T2616	11041-11045	VBD	denotes	were
T2615	11046-11054	VBN	denotes	detected
T2617	11055-11057	IN	denotes	on
T2618	11058-11061	DT	denotes	the
T2619	11062-11070	NNP	denotes	Southern
T2620	11071-11074	CC	denotes	and
T2621	11075-11077	DT	denotes	no
T2622	11078-11083	NNS	denotes	bands
T2624	11084-11088	VBD	denotes	were
T2623	11089-11097	VBN	denotes	detected
T2625	11098-11100	IN	denotes	on
T2626	11101-11104	DT	denotes	the
T2627	11105-11113	NNP	denotes	Northern
T2628	11113-11115	,	denotes	,
T2629	11115-11125	VBG	denotes	confirming
T2630	11126-11130	IN	denotes	that
T2632	11131-11135	DT	denotes	this
T2634	11136-11147	JJ	denotes	alternative
T2633	11148-11158	NN	denotes	transcript
T2631	11159-11161	VBZ	denotes	is
T2635	11162-11170	JJ	denotes	specific
T2636	11171-11173	IN	denotes	to
T2637	11174-11180	JJ	denotes	murine
T2638	11181-11187	NN	denotes	Mcoln1
T2639	11187-11188	.	denotes	.
T2640	11188-11356	sentence	denotes	Recent BLASTP analysis of the alternate Mcoln1 transcript yields a match to a putative 145 amino acid anonymous protein (GB No. BAB25862) predicted from a RIKEN clone.
T2641	11189-11195	JJ	denotes	Recent
T2643	11196-11202	NN	denotes	BLASTP
T2642	11203-11211	NN	denotes	analysis
T2645	11212-11214	IN	denotes	of
T2646	11215-11218	DT	denotes	the
T2648	11219-11228	JJ	denotes	alternate
T2649	11229-11235	NN	denotes	Mcoln1
T2647	11236-11246	NN	denotes	transcript
T2644	11247-11253	VBZ	denotes	yields
T2650	11254-11255	DT	denotes	a
T2651	11256-11261	NN	denotes	match
T2652	11262-11264	IN	denotes	to
T2653	11265-11266	DT	denotes	a
T2655	11267-11275	JJ	denotes	putative
T2656	11276-11279	CD	denotes	145
T2658	11280-11285	NN	denotes	amino
T2657	11286-11290	NN	denotes	acid
T2659	11291-11300	JJ	denotes	anonymous
T2654	11301-11308	NN	denotes	protein
T2660	11309-11310	-LRB-	denotes	(
T2662	11310-11312	NN	denotes	GB
T2663	11313-11316	NN	denotes	No.
T2661	11317-11325	NN	denotes	BAB25862
T2664	11325-11326	-RRB-	denotes	)
T2665	11327-11336	VBN	denotes	predicted
T2666	11337-11341	IN	denotes	from
T2667	11342-11343	DT	denotes	a
T2669	11344-11349	NN	denotes	RIKEN
T2668	11350-11355	NN	denotes	clone
T2670	11355-11356	.	denotes	.
T2671	11356-11598	sentence	denotes	It is obvious from our results, however, that the identification of this sequence as a full-length protein is incorrect since probes unique to the clone, as well as probes containing the Mcoln1 coding sequence, identify the same transcripts.
T2672	11357-11359	PRP	denotes	It
T2673	11360-11362	VBZ	denotes	is
T2674	11363-11370	JJ	denotes	obvious
T2675	11371-11375	IN	denotes	from
T2676	11376-11379	PRP$	denotes	our
T2677	11380-11387	NNS	denotes	results
T2678	11387-11389	,	denotes	,
T2679	11389-11396	RB	denotes	however
T2680	11396-11398	,	denotes	,
T2681	11398-11402	IN	denotes	that
T2683	11403-11406	DT	denotes	the
T2684	11407-11421	NN	denotes	identification
T2685	11422-11424	IN	denotes	of
T2686	11425-11429	DT	denotes	this
T2687	11430-11438	NN	denotes	sequence
T2688	11439-11441	IN	denotes	as
T2689	11442-11443	DT	denotes	a
T2691	11444-11448	JJ	denotes	full
T2693	11448-11449	HYPH	denotes	-
T2692	11449-11455	NN	denotes	length
T2690	11456-11463	NN	denotes	protein
T2682	11464-11466	VBZ	denotes	is
T2694	11467-11476	JJ	denotes	incorrect
T2695	11477-11482	IN	denotes	since
T2697	11483-11489	NNS	denotes	probes
T2698	11490-11496	JJ	denotes	unique
T2699	11497-11499	IN	denotes	to
T2700	11500-11503	DT	denotes	the
T2701	11504-11509	NN	denotes	clone
T2702	11509-11511	,	denotes	,
T2703	11511-11513	RB	denotes	as
T2705	11514-11518	RB	denotes	well
T2704	11519-11521	IN	denotes	as
T2706	11522-11528	NNS	denotes	probes
T2707	11529-11539	VBG	denotes	containing
T2708	11540-11543	DT	denotes	the
T2710	11544-11550	NN	denotes	Mcoln1
T2711	11551-11557	NN	denotes	coding
T2709	11558-11566	NN	denotes	sequence
T2712	11566-11568	,	denotes	,
T2696	11568-11576	VBP	denotes	identify
T2713	11577-11580	DT	denotes	the
T2715	11581-11585	JJ	denotes	same
T2714	11586-11597	NNS	denotes	transcripts
T2716	11597-11598	.	denotes	.
T3016	11612-11622	NN	denotes	Comparison
T3018	11623-11625	IN	denotes	of
T3019	11626-11632	NN	denotes	Mcoln1
T3020	11633-11640	NN	denotes	isoform
T3021	11641-11642	CD	denotes	1
T3022	11643-11645	IN	denotes	to
T3023	11646-11649	PRP$	denotes	its
T3025	11650-11655	JJ	denotes	human
T3024	11656-11665	NN	denotes	homologue
T3017	11666-11671	VBZ	denotes	shows
T3026	11672-11680	JJ	denotes	striking
T3027	11681-11691	NN	denotes	similarity
T3028	11692-11694	IN	denotes	at
T3029	11695-11699	CC	denotes	both
T3031	11700-11703	DT	denotes	the
T3032	11704-11709	NN	denotes	amino
T3033	11710-11714	NN	denotes	acid
T3034	11715-11718	CC	denotes	and
T3035	11719-11729	NN	denotes	nucleotide
T3030	11730-11735	NN	denotes	level
T3036	11735-11736	.	denotes	.
T3037	11736-11835	sentence	denotes	All six of the transmembrane domains, as well as the putative cation channel are highly conserved.
T3038	11737-11740	DT	denotes	All
T3039	11741-11744	CD	denotes	six
T3041	11745-11747	IN	denotes	of
T3042	11748-11751	DT	denotes	the
T3044	11752-11765	NN	denotes	transmembrane
T3043	11766-11773	NNS	denotes	domains
T3045	11773-11775	,	denotes	,
T3046	11775-11777	RB	denotes	as
T3048	11778-11782	RB	denotes	well
T3047	11783-11785	IN	denotes	as
T3049	11786-11789	DT	denotes	the
T3051	11790-11798	JJ	denotes	putative
T3052	11799-11805	NN	denotes	cation
T3050	11806-11813	NN	denotes	channel
T3053	11814-11817	VBP	denotes	are
T3054	11818-11824	RB	denotes	highly
T3040	11825-11834	VBN	denotes	conserved
T3055	11834-11835	.	denotes	.
T3056	11835-11979	sentence	denotes	The putative di-leucine (L-L-X-X) motif at the C-terminus, which may act as a late endosomal/lysosomal targeting signal, is also conserved [9].
T3057	11836-11839	DT	denotes	The
T3059	11840-11848	JJ	denotes	putative
T3060	11849-11859	NN	denotes	di-leucine
T3061	11860-11861	-LRB-	denotes	(
T3063	11861-11862	NN	denotes	L
T3064	11862-11863	HYPH	denotes	-
T3065	11863-11864	NN	denotes	L
T3066	11864-11865	HYPH	denotes	-
T3067	11865-11866	NN	denotes	X
T3068	11866-11867	HYPH	denotes	-
T3062	11867-11868	NN	denotes	X
T3069	11868-11869	-RRB-	denotes	)
T3058	11870-11875	NN	denotes	motif
T3071	11876-11878	IN	denotes	at
T3072	11879-11882	DT	denotes	the
T3074	11883-11884	NN	denotes	C
T3075	11884-11885	HYPH	denotes	-
T3073	11885-11893	NN	denotes	terminus
T3076	11893-11895	,	denotes	,
T3077	11895-11900	WDT	denotes	which
T3079	11901-11904	MD	denotes	may
T3078	11905-11908	VB	denotes	act
T3080	11909-11911	IN	denotes	as
T3081	11912-11913	DT	denotes	a
T3083	11914-11918	JJ	denotes	late
T3084	11919-11928	JJ	denotes	endosomal
T3086	11928-11929	HYPH	denotes	/
T3085	11929-11938	JJ	denotes	lysosomal
T3087	11939-11948	NN	denotes	targeting
T3082	11949-11955	NN	denotes	signal
T3088	11955-11957	,	denotes	,
T3089	11957-11959	VBZ	denotes	is
T3090	11960-11964	RB	denotes	also
T3070	11965-11974	VBN	denotes	conserved
T3091	11975-11976	-LRB-	denotes	[
T3092	11976-11977	CD	denotes	9
T3093	11977-11978	-RRB-	denotes	]
T3094	11978-11979	.	denotes	.
T3095	11979-12179	sentence	denotes	This speculation is supported by work with cup-5[13], the c. elegans homologue of MCOLN1, since cellular localization studies suggest that the protein is found in the late endosomes and/or lysosomes.
T3096	11980-11984	DT	denotes	This
T3097	11985-11996	NN	denotes	speculation
T3099	11997-11999	VBZ	denotes	is
T3098	12000-12009	VBN	denotes	supported
T3100	12010-12012	IN	denotes	by
T3101	12013-12017	NN	denotes	work
T3102	12018-12022	IN	denotes	with
T3103	12023-12026	NN	denotes	cup
T3105	12026-12027	HYPH	denotes	-
T3104	12027-12028	NN	denotes	5
T3106	12028-12029	-LRB-	denotes	[
T3107	12029-12031	CD	denotes	13
T3108	12031-12032	-RRB-	denotes	]
T3109	12032-12034	,	denotes	,
T3110	12034-12037	DT	denotes	the
T3112	12038-12040	NNP	denotes	c.
T3113	12041-12048	NNP	denotes	elegans
T3111	12049-12058	NN	denotes	homologue
T3114	12059-12061	IN	denotes	of
T3115	12062-12068	NN	denotes	MCOLN1
T3116	12068-12070	,	denotes	,
T3117	12070-12075	IN	denotes	since
T3119	12076-12084	JJ	denotes	cellular
T3120	12085-12097	NN	denotes	localization
T3121	12098-12105	NNS	denotes	studies
T3118	12106-12113	VBP	denotes	suggest
T3122	12114-12118	IN	denotes	that
T3124	12119-12122	DT	denotes	the
T3125	12123-12130	NN	denotes	protein
T3126	12131-12133	VBZ	denotes	is
T3123	12134-12139	VBN	denotes	found
T3127	12140-12142	IN	denotes	in
T3128	12143-12146	DT	denotes	the
T3130	12147-12151	JJ	denotes	late
T3129	12152-12161	NNS	denotes	endosomes
T3131	12162-12165	CC	denotes	and
T3132	12165-12166	HYPH	denotes	/
T3133	12166-12168	CC	denotes	or
T3134	12169-12178	NNS	denotes	lysosomes
T3135	12178-12179	.	denotes	.
T3136	12179-12304	sentence	denotes	The mouse Mcoln1 gene has two alternatively spliced isoforms, with isoform 2 having a different c-terminal cytoplasmic tail.
T3137	12180-12183	DT	denotes	The
T3139	12184-12189	NN	denotes	mouse
T3140	12190-12196	NN	denotes	Mcoln1
T3138	12197-12201	NN	denotes	gene
T3141	12202-12205	VBZ	denotes	has
T3142	12206-12209	CD	denotes	two
T3144	12210-12223	RB	denotes	alternatively
T3145	12224-12231	VBN	denotes	spliced
T3143	12232-12240	NNS	denotes	isoforms
T3146	12240-12242	,	denotes	,
T3147	12242-12246	IN	denotes	with
T3148	12247-12254	NN	denotes	isoform
T3150	12255-12256	CD	denotes	2
T3149	12257-12263	VBG	denotes	having
T3151	12264-12265	DT	denotes	a
T3153	12266-12275	JJ	denotes	different
T3154	12276-12277	NN	denotes	c
T3156	12277-12278	HYPH	denotes	-
T3155	12278-12286	JJ	denotes	terminal
T3157	12287-12298	JJ	denotes	cytoplasmic
T3152	12299-12303	NN	denotes	tail
T3158	12303-12304	.	denotes	.
T3159	12304-12478	sentence	denotes	The unique 86 amino acid c-terminal tail lacks the lysosomal targeting signal and does not contain any conserved domains when compared against the current profile databases.
T3160	12305-12308	DT	denotes	The
T3162	12309-12315	JJ	denotes	unique
T3163	12316-12318	CD	denotes	86
T3165	12319-12324	NN	denotes	amino
T3164	12325-12329	NN	denotes	acid
T3166	12330-12331	NN	denotes	c
T3168	12331-12332	HYPH	denotes	-
T3167	12332-12340	JJ	denotes	terminal
T3161	12341-12345	NN	denotes	tail
T3169	12346-12351	VBZ	denotes	lacks
T3170	12352-12355	DT	denotes	the
T3172	12356-12365	JJ	denotes	lysosomal
T3173	12366-12375	NN	denotes	targeting
T3171	12376-12382	NN	denotes	signal
T3174	12383-12386	CC	denotes	and
T3175	12387-12391	VBZ	denotes	does
T3177	12392-12395	RB	denotes	not
T3176	12396-12403	VB	denotes	contain
T3178	12404-12407	DT	denotes	any
T3180	12408-12417	VBN	denotes	conserved
T3179	12418-12425	NNS	denotes	domains
T3181	12426-12430	WRB	denotes	when
T3182	12431-12439	VBN	denotes	compared
T3183	12440-12447	IN	denotes	against
T3184	12448-12451	DT	denotes	the
T3186	12452-12459	JJ	denotes	current
T3187	12460-12467	NN	denotes	profile
T3185	12468-12477	NNS	denotes	databases
T3188	12477-12478	.	denotes	.
T3189	12478-12655	sentence	denotes	We speculate that this protein may have similar channel function but an alternate subcellular localization, but this must be proven once isoform-specific antibodies are raised.
T3190	12479-12481	PRP	denotes	We
T3191	12482-12491	VBP	denotes	speculate
T3192	12492-12496	IN	denotes	that
T3194	12497-12501	DT	denotes	this
T3195	12502-12509	NN	denotes	protein
T3196	12510-12513	MD	denotes	may
T3193	12514-12518	VB	denotes	have
T3197	12519-12526	JJ	denotes	similar
T3199	12527-12534	NN	denotes	channel
T3198	12535-12543	NN	denotes	function
T3200	12544-12547	CC	denotes	but
T3201	12548-12550	DT	denotes	an
T3203	12551-12560	JJ	denotes	alternate
T3204	12561-12572	JJ	denotes	subcellular
T3202	12573-12585	NN	denotes	localization
T3205	12585-12587	,	denotes	,
T3206	12587-12590	CC	denotes	but
T3207	12591-12595	DT	denotes	this
T3209	12596-12600	MD	denotes	must
T3210	12601-12603	VB	denotes	be
T3208	12604-12610	VBN	denotes	proven
T3211	12611-12615	IN	denotes	once
T3213	12616-12623	NN	denotes	isoform
T3215	12623-12624	HYPH	denotes	-
T3214	12624-12632	JJ	denotes	specific
T3216	12633-12643	NNS	denotes	antibodies
T3217	12644-12647	VBP	denotes	are
T3212	12648-12654	VBN	denotes	raised
T3218	12654-12655	.	denotes	.
T3219	12655-12846	sentence	denotes	However, our results suggest that phenotypic assessment of Mcoln1 knock-out mice may be complicated and that care must be taken when interpreting data on mouse gene expression and phenotype.
T3220	12656-12663	RB	denotes	However
T3222	12663-12665	,	denotes	,
T3223	12665-12668	PRP$	denotes	our
T3224	12669-12676	NNS	denotes	results
T3221	12677-12684	VBP	denotes	suggest
T3225	12685-12689	IN	denotes	that
T3227	12690-12700	JJ	denotes	phenotypic
T3228	12701-12711	NN	denotes	assessment
T3229	12712-12714	IN	denotes	of
T3230	12715-12721	NN	denotes	Mcoln1
T3232	12722-12727	NN	denotes	knock
T3234	12727-12728	HYPH	denotes	-
T3233	12728-12731	NN	denotes	out
T3231	12732-12736	NNS	denotes	mice
T3235	12737-12740	MD	denotes	may
T3226	12741-12743	VB	denotes	be
T3236	12744-12755	JJ	denotes	complicated
T3237	12756-12759	CC	denotes	and
T3238	12760-12764	IN	denotes	that
T3240	12765-12769	NN	denotes	care
T3241	12770-12774	MD	denotes	must
T3242	12775-12777	VB	denotes	be
T3239	12778-12783	VBN	denotes	taken
T3243	12784-12788	WRB	denotes	when
T3244	12789-12801	VBG	denotes	interpreting
T3245	12802-12806	NNS	denotes	data
T3246	12807-12809	IN	denotes	on
T3247	12810-12815	NN	denotes	mouse
T3249	12816-12820	NN	denotes	gene
T3248	12821-12831	NN	denotes	expression
T3250	12832-12835	CC	denotes	and
T3251	12836-12845	NN	denotes	phenotype
T3252	12845-12846	.	denotes	.
T3253	12846-13002	sentence	denotes	Interestingly, the second Mcoln1 isoform is not seen in humans and the sequence of the alternatively spliced region is not conserved between man and mouse.
T3254	12847-12860	RB	denotes	Interestingly
T3256	12860-12862	,	denotes	,
T3257	12862-12865	DT	denotes	the
T3259	12866-12872	JJ	denotes	second
T3260	12873-12879	NN	denotes	Mcoln1
T3258	12880-12887	NN	denotes	isoform
T3261	12888-12890	VBZ	denotes	is
T3262	12891-12894	RB	denotes	not
T3255	12895-12899	VBN	denotes	seen
T3263	12900-12902	IN	denotes	in
T3264	12903-12909	NNS	denotes	humans
T3265	12910-12913	CC	denotes	and
T3266	12914-12917	DT	denotes	the
T3267	12918-12926	NN	denotes	sequence
T3269	12927-12929	IN	denotes	of
T3270	12930-12933	DT	denotes	the
T3272	12934-12947	RB	denotes	alternatively
T3273	12948-12955	VBN	denotes	spliced
T3271	12956-12962	NN	denotes	region
T3274	12963-12965	VBZ	denotes	is
T3275	12966-12969	RB	denotes	not
T3268	12970-12979	VBN	denotes	conserved
T3276	12980-12987	IN	denotes	between
T3277	12988-12991	NN	denotes	man
T3278	12992-12995	CC	denotes	and
T3279	12996-13001	NN	denotes	mouse
T3280	13001-13002	.	denotes	.
T3281	13002-13101	sentence	denotes	To date, very few genes have been reported that show species specific alternative splice variants.
T3282	13003-13005	IN	denotes	To
T3284	13006-13010	NN	denotes	date
T3285	13010-13012	,	denotes	,
T3286	13012-13016	RB	denotes	very
T3287	13017-13020	JJ	denotes	few
T3288	13021-13026	NNS	denotes	genes
T3289	13027-13031	VBP	denotes	have
T3290	13032-13036	VBN	denotes	been
T3283	13037-13045	VBN	denotes	reported
T3291	13046-13050	WDT	denotes	that
T3292	13051-13055	VBP	denotes	show
T3293	13056-13063	NN	denotes	species
T3294	13064-13072	JJ	denotes	specific
T3296	13073-13084	JJ	denotes	alternative
T3297	13085-13091	NN	denotes	splice
T3295	13092-13100	NNS	denotes	variants
T3298	13100-13101	.	denotes	.
T3299	13101-13221	sentence	denotes	MOG, myelin/oligodendrocyte glycoprotein, has many different splice variants in humans that are not found in mice [17].
T3300	13102-13105	NN	denotes	MOG
T3302	13105-13107	,	denotes	,
T3303	13107-13113	NN	denotes	myelin
T3305	13113-13114	HYPH	denotes	/
T3304	13114-13129	NN	denotes	oligodendrocyte
T3306	13130-13142	NN	denotes	glycoprotein
T3307	13142-13144	,	denotes	,
T3301	13144-13147	VBZ	denotes	has
T3308	13148-13152	JJ	denotes	many
T3310	13153-13162	JJ	denotes	different
T3311	13163-13169	NN	denotes	splice
T3309	13170-13178	NNS	denotes	variants
T3312	13179-13181	IN	denotes	in
T3313	13182-13188	NNS	denotes	humans
T3314	13189-13193	WDT	denotes	that
T3316	13194-13197	VBP	denotes	are
T3317	13198-13201	RB	denotes	not
T3315	13202-13207	VBN	denotes	found
T3318	13208-13210	IN	denotes	in
T3319	13211-13215	NNS	denotes	mice
T3320	13216-13217	-LRB-	denotes	[
T3321	13217-13219	CD	denotes	17
T3322	13219-13220	-RRB-	denotes	]
T3323	13220-13221	.	denotes	.
T3324	13221-13390	sentence	denotes	ATP11B, a P-type ATPase, has a rabbit-specific splice variant that deletes a transmembrane domain and therefore likely alters the putative function of the protein [18].
T3325	13222-13228	NN	denotes	ATP11B
T3327	13228-13230	,	denotes	,
T3328	13230-13231	DT	denotes	a
T3330	13232-13233	NN	denotes	P
T3332	13233-13234	HYPH	denotes	-
T3331	13234-13238	NN	denotes	type
T3329	13239-13245	NN	denotes	ATPase
T3333	13245-13247	,	denotes	,
T3326	13247-13250	VBZ	denotes	has
T3334	13251-13252	DT	denotes	a
T3336	13253-13259	NN	denotes	rabbit
T3338	13259-13260	HYPH	denotes	-
T3337	13260-13268	JJ	denotes	specific
T3339	13269-13275	NN	denotes	splice
T3335	13276-13283	NN	denotes	variant
T3340	13284-13288	WDT	denotes	that
T3341	13289-13296	VBZ	denotes	deletes
T3342	13297-13298	DT	denotes	a
T3344	13299-13312	JJ	denotes	transmembrane
T3343	13313-13319	NN	denotes	domain
T3345	13320-13323	CC	denotes	and
T3346	13324-13333	RB	denotes	therefore
T3348	13334-13340	RB	denotes	likely
T3347	13341-13347	VBZ	denotes	alters
T3349	13348-13351	DT	denotes	the
T3351	13352-13360	JJ	denotes	putative
T3350	13361-13369	NN	denotes	function
T3352	13370-13372	IN	denotes	of
T3353	13373-13376	DT	denotes	the
T3354	13377-13384	NN	denotes	protein
T3355	13385-13386	-LRB-	denotes	[
T3356	13386-13388	CD	denotes	18
T3357	13388-13389	-RRB-	denotes	]
T3358	13389-13390	.	denotes	.
T3359	13390-13629	sentence	denotes	Sequencing of the human genome has led to estimates of approximately 32,000 genes, a total surprise given the previous significantly higher estimates that were based on the number of expressed sequence tags (ESTs) in the public databases.
T3360	13391-13401	NN	denotes	Sequencing
T3362	13402-13404	IN	denotes	of
T3363	13405-13408	DT	denotes	the
T3365	13409-13414	JJ	denotes	human
T3364	13415-13421	NN	denotes	genome
T3366	13422-13425	VBZ	denotes	has
T3361	13426-13429	VBN	denotes	led
T3367	13430-13432	IN	denotes	to
T3368	13433-13442	NNS	denotes	estimates
T3369	13443-13445	IN	denotes	of
T3370	13446-13459	RB	denotes	approximately
T3371	13460-13466	CD	denotes	32,000
T3372	13467-13472	NNS	denotes	genes
T3373	13472-13474	,	denotes	,
T3374	13474-13475	DT	denotes	a
T3376	13476-13481	JJ	denotes	total
T3375	13482-13490	NN	denotes	surprise
T3377	13491-13496	VBN	denotes	given
T3378	13497-13500	DT	denotes	the
T3380	13501-13509	JJ	denotes	previous
T3381	13510-13523	RB	denotes	significantly
T3382	13524-13530	JJR	denotes	higher
T3379	13531-13540	NNS	denotes	estimates
T3383	13541-13545	WDT	denotes	that
T3385	13546-13550	VBD	denotes	were
T3384	13551-13556	VBN	denotes	based
T3386	13557-13559	IN	denotes	on
T3387	13560-13563	DT	denotes	the
T3388	13564-13570	NN	denotes	number
T3389	13571-13573	IN	denotes	of
T3390	13574-13583	VBN	denotes	expressed
T3392	13584-13592	NN	denotes	sequence
T3391	13593-13597	NNS	denotes	tags
T3393	13598-13599	-LRB-	denotes	(
T3394	13599-13603	NNS	denotes	ESTs
T3395	13603-13604	-RRB-	denotes	)
T3396	13605-13607	IN	denotes	in
T3397	13608-13611	DT	denotes	the
T3399	13612-13618	JJ	denotes	public
T3398	13619-13628	NNS	denotes	databases
T3400	13628-13629	.	denotes	.
T3401	13629-13821	sentence	denotes	This apparent disparity suggests a major role for alternative splicing in creating genetic complexity, and has brought the study of splicing regulation to the forefront of molecular genetics.
T3402	13630-13634	DT	denotes	This
T3404	13635-13643	JJ	denotes	apparent
T3403	13644-13653	NN	denotes	disparity
T3405	13654-13662	VBZ	denotes	suggests
T3406	13663-13664	DT	denotes	a
T3408	13665-13670	JJ	denotes	major
T3407	13671-13675	NN	denotes	role
T3409	13676-13679	IN	denotes	for
T3410	13680-13691	JJ	denotes	alternative
T3411	13692-13700	NN	denotes	splicing
T3412	13701-13703	IN	denotes	in
T3413	13704-13712	VBG	denotes	creating
T3414	13713-13720	JJ	denotes	genetic
T3415	13721-13731	NN	denotes	complexity
T3416	13731-13733	,	denotes	,
T3417	13733-13736	CC	denotes	and
T3418	13737-13740	VBZ	denotes	has
T3419	13741-13748	VBN	denotes	brought
T3420	13749-13752	DT	denotes	the
T3421	13753-13758	NN	denotes	study
T3422	13759-13761	IN	denotes	of
T3423	13762-13770	NN	denotes	splicing
T3424	13771-13781	NN	denotes	regulation
T3425	13782-13784	IN	denotes	to
T3426	13785-13788	DT	denotes	the
T3427	13789-13798	NN	denotes	forefront
T3428	13799-13801	IN	denotes	of
T3429	13802-13811	JJ	denotes	molecular
T3430	13812-13820	NN	denotes	genetics
T3431	13820-13821	.	denotes	.
T3432	13821-13980	sentence	denotes	It is likely that an abundance of species-specific splice variants will be identified as the characterization of alternatively spliced transcripts progresses.
T3433	13822-13824	PRP	denotes	It
T3434	13825-13827	VBZ	denotes	is
T3435	13828-13834	JJ	denotes	likely
T3436	13835-13839	IN	denotes	that
T3438	13840-13842	DT	denotes	an
T3439	13843-13852	NN	denotes	abundance
T3440	13853-13855	IN	denotes	of
T3441	13856-13863	NN	denotes	species
T3443	13863-13864	HYPH	denotes	-
T3442	13864-13872	JJ	denotes	specific
T3445	13873-13879	NN	denotes	splice
T3444	13880-13888	NNS	denotes	variants
T3446	13889-13893	MD	denotes	will
T3447	13894-13896	VB	denotes	be
T3437	13897-13907	VBN	denotes	identified
T3448	13908-13910	IN	denotes	as
T3450	13911-13914	DT	denotes	the
T3451	13915-13931	NN	denotes	characterization
T3452	13932-13934	IN	denotes	of
T3453	13935-13948	RB	denotes	alternatively
T3454	13949-13956	VBN	denotes	spliced
T3455	13957-13968	NNS	denotes	transcripts
T3449	13969-13979	VBZ	denotes	progresses
T3456	13979-13980	.	denotes	.
T3567	14005-14019	NN	denotes	Bioinformatics
T3568	14019-14096	sentence	denotes	We conducted database searches using BLAST and Draft Human Genome Browser .
T3569	14020-14022	PRP	denotes	We
T3570	14023-14032	VBD	denotes	conducted
T3571	14033-14041	NN	denotes	database
T3572	14042-14050	NNS	denotes	searches
T3573	14051-14056	VBG	denotes	using
T3574	14057-14062	NNP	denotes	BLAST
T3575	14064-14067	CC	denotes	and
T3576	14068-14073	NNP	denotes	Draft
T3578	14074-14079	NNP	denotes	Human
T3579	14080-14086	NNP	denotes	Genome
T3577	14087-14094	NNP	denotes	Browser
T3580	14095-14096	.	denotes	.
T3581	14096-14162	sentence	denotes	Sequences from UniGene were used to confirm the Mcoln1 sequence.
T3582	14097-14106	NNS	denotes	Sequences
T3584	14107-14111	IN	denotes	from
T3585	14112-14119	NNP	denotes	UniGene
T3586	14121-14125	VBD	denotes	were
T3583	14126-14130	VBN	denotes	used
T3587	14131-14133	TO	denotes	to
T3588	14134-14141	VB	denotes	confirm
T3589	14142-14145	DT	denotes	the
T3591	14146-14152	NN	denotes	Mcoln1
T3590	14153-14161	NN	denotes	sequence
T3592	14161-14162	.	denotes	.
T3593	14162-14290	sentence	denotes	We performed motif searches using ProfileScan and TMPred and alignment of protein sequences using Pileup (GCG) and Boxshade .
T3594	14163-14165	PRP	denotes	We
T3595	14166-14175	VBD	denotes	performed
T3596	14176-14181	NN	denotes	motif
T3597	14182-14190	NNS	denotes	searches
T3598	14191-14196	VBG	denotes	using
T3599	14197-14208	NN	denotes	ProfileScan
T3600	14210-14213	CC	denotes	and
T3601	14214-14220	NN	denotes	TMPred
T3602	14222-14225	CC	denotes	and
T3603	14226-14235	NN	denotes	alignment
T3604	14236-14238	IN	denotes	of
T3605	14239-14246	NN	denotes	protein
T3606	14247-14256	NNS	denotes	sequences
T3607	14257-14262	VBG	denotes	using
T3608	14263-14269	NN	denotes	Pileup
T3609	14270-14271	-LRB-	denotes	(
T3610	14271-14274	NN	denotes	GCG
T3611	14274-14275	-RRB-	denotes	)
T3612	14276-14279	CC	denotes	and
T3613	14280-14288	NNP	denotes	Boxshade
T3614	14289-14290	.	denotes	.
T3623	14292-14295	NN	denotes	DNA
T3624	14296-14306	NN	denotes	Sequencing
T3625	14306-14380	sentence	denotes	I.M.A.G.E. Clones were purchased from Research Genetics (Huntsville, AL).
T3626	14307-14317	NN	denotes	I.M.A.G.E.
T3627	14318-14324	NNS	denotes	Clones
T3629	14325-14329	VBD	denotes	were
T3628	14330-14339	VBN	denotes	purchased
T3630	14340-14344	IN	denotes	from
T3631	14345-14353	NNP	denotes	Research
T3632	14354-14362	NNP	denotes	Genetics
T3633	14363-14364	-LRB-	denotes	(
T3634	14364-14374	NNP	denotes	Huntsville
T3635	14374-14376	,	denotes	,
T3636	14376-14378	NNP	denotes	AL
T3637	14378-14379	-RRB-	denotes	)
T3638	14379-14380	.	denotes	.
T3639	14380-14527	sentence	denotes	Sequencing was performed using the AmpliCycle sequencing kit from Applied Biosystems (Foster City, CA) on a Genomyx LR programmable DNA sequencer.
T3640	14381-14391	NN	denotes	Sequencing
T3642	14392-14395	VBD	denotes	was
T3641	14396-14405	VBN	denotes	performed
T3643	14406-14411	VBG	denotes	using
T3644	14412-14415	DT	denotes	the
T3646	14416-14426	NN	denotes	AmpliCycle
T3647	14427-14437	NN	denotes	sequencing
T3645	14438-14441	NN	denotes	kit
T3648	14442-14446	IN	denotes	from
T3649	14447-14454	NNP	denotes	Applied
T3650	14455-14465	NNP	denotes	Biosystems
T3651	14466-14467	-LRB-	denotes	(
T3653	14467-14473	NNP	denotes	Foster
T3652	14474-14478	NNP	denotes	City
T3654	14478-14480	,	denotes	,
T3655	14480-14482	NNP	denotes	CA
T3656	14482-14483	-RRB-	denotes	)
T3657	14484-14486	IN	denotes	on
T3658	14487-14488	DT	denotes	a
T3660	14489-14496	NN	denotes	Genomyx
T3661	14497-14499	NN	denotes	LR
T3662	14500-14512	JJ	denotes	programmable
T3663	14513-14516	NN	denotes	DNA
T3659	14517-14526	NN	denotes	sequencer
T3664	14526-14527	.	denotes	.
T3689	14529-14531	NN	denotes	RT
T3691	14531-14532	HYPH	denotes	-
T3690	14532-14535	NN	denotes	PCR
T3692	14536-14544	NN	denotes	Reaction
T3693	14544-14669	sentence	denotes	BALB/c mouse brain total RNA was purchased from Clontech Laboratories (Palo Alto, CA) and made to a 1 μg / ml concentration.
T3694	14545-14549	NN	denotes	BALB
T3696	14549-14550	HYPH	denotes	/
T3695	14550-14551	NN	denotes	c
T3697	14552-14557	NN	denotes	mouse
T3698	14558-14563	NN	denotes	brain
T3700	14564-14569	JJ	denotes	total
T3699	14570-14573	NN	denotes	RNA
T3702	14574-14577	VBD	denotes	was
T3701	14578-14587	VBN	denotes	purchased
T3703	14588-14592	IN	denotes	from
T3704	14593-14601	NNP	denotes	Clontech
T3705	14602-14614	NNP	denotes	Laboratories
T3706	14615-14616	-LRB-	denotes	(
T3708	14616-14620	NNP	denotes	Palo
T3707	14621-14625	NNP	denotes	Alto
T3709	14625-14627	,	denotes	,
T3710	14627-14629	NNP	denotes	CA
T3711	14629-14630	-RRB-	denotes	)
T3712	14631-14634	CC	denotes	and
T3713	14635-14639	VBN	denotes	made
T3714	14640-14642	IN	denotes	to
T3715	14643-14644	DT	denotes	a
T3717	14645-14646	CD	denotes	1
T3718	14647-14649	NN	denotes	μg
T3719	14650-14651	SYM	denotes	/
T3720	14652-14654	NN	denotes	ml
T3716	14655-14668	NN	denotes	concentration
T3721	14668-14669	.	denotes	.
T3722	14669-14770	sentence	denotes	This RNA was used as a template to create cDNA via RT-PCR using random hexamers and oligo dT primer.
T3723	14670-14674	DT	denotes	This
T3724	14675-14678	NN	denotes	RNA
T3726	14679-14682	VBD	denotes	was
T3725	14683-14687	VBN	denotes	used
T3727	14688-14690	IN	denotes	as
T3728	14691-14692	DT	denotes	a
T3729	14693-14701	NN	denotes	template
T3730	14702-14704	TO	denotes	to
T3731	14705-14711	VB	denotes	create
T3732	14712-14716	NN	denotes	cDNA
T3733	14717-14720	IN	denotes	via
T3734	14721-14723	NN	denotes	RT
T3736	14723-14724	HYPH	denotes	-
T3735	14724-14727	NN	denotes	PCR
T3737	14728-14733	VBG	denotes	using
T3738	14734-14740	JJ	denotes	random
T3739	14741-14749	NNS	denotes	hexamers
T3740	14750-14753	CC	denotes	and
T3741	14754-14759	NN	denotes	oligo
T3743	14760-14762	NN	denotes	dT
T3742	14763-14769	NN	denotes	primer
T3744	14769-14770	.	denotes	.
T3745	14770-14984	sentence	denotes	The RT product was used to confirm the alternatively spliced form using primers: 5'-CATCTACCTGGGCTATTGC-3' (forward) and 5'-GCTCTCAGGTGGTGGACAC-3' (reverse) in a PCR reaction with an annealing temperature of 61°C.
T3746	14771-14774	DT	denotes	The
T3748	14775-14777	NN	denotes	RT
T3747	14778-14785	NN	denotes	product
T3750	14786-14789	VBD	denotes	was
T3749	14790-14794	VBN	denotes	used
T3751	14795-14797	TO	denotes	to
T3752	14798-14805	VB	denotes	confirm
T3753	14806-14809	DT	denotes	the
T3755	14810-14823	RB	denotes	alternatively
T3756	14824-14831	VBN	denotes	spliced
T3754	14832-14836	NN	denotes	form
T3757	14837-14842	VBG	denotes	using
T3758	14843-14850	NNS	denotes	primers
T3759	14850-14852	:	denotes	:
T3760	14852-14853	CD	denotes	5
T3762	14853-14854	SYM	denotes	'
T3763	14854-14855	HYPH	denotes	-
T3764	14855-14874	NN	denotes	CATCTACCTGGGCTATTGC
T3765	14874-14875	HYPH	denotes	-
T3761	14875-14876	NN	denotes	3
T3766	14876-14877	SYM	denotes	'
T3767	14878-14879	-LRB-	denotes	(
T3768	14879-14886	RB	denotes	forward
T3769	14886-14887	-RRB-	denotes	)
T3770	14888-14891	CC	denotes	and
T3771	14892-14893	CD	denotes	5
T3773	14893-14894	SYM	denotes	'
T3774	14894-14895	HYPH	denotes	-
T3775	14895-14914	NN	denotes	GCTCTCAGGTGGTGGACAC
T3776	14914-14915	HYPH	denotes	-
T3772	14915-14916	NN	denotes	3
T3777	14916-14917	SYM	denotes	'
T3778	14918-14919	-LRB-	denotes	(
T3779	14919-14926	RB	denotes	reverse
T3780	14926-14927	-RRB-	denotes	)
T3781	14928-14930	IN	denotes	in
T3782	14931-14932	DT	denotes	a
T3784	14933-14936	NN	denotes	PCR
T3783	14937-14945	NN	denotes	reaction
T3785	14946-14950	IN	denotes	with
T3786	14951-14953	DT	denotes	an
T3788	14954-14963	JJ	denotes	annealing
T3787	14964-14975	NN	denotes	temperature
T3789	14976-14978	IN	denotes	of
T3790	14979-14981	CD	denotes	61
T3791	14981-14983	NNS	denotes	°C
T3792	14983-14984	.	denotes	.
T3835	14986-14994	NNP	denotes	Southern
T3836	14995-14999	NN	denotes	Blot
T3837	15000-15008	NN	denotes	Analysis
T3838	15008-15081	sentence	denotes	Total mouse genomic DNA was digested using EcoRI, BamHI, PstI, and XbaI.
T3839	15009-15014	JJ	denotes	Total
T3841	15015-15020	NN	denotes	mouse
T3842	15021-15028	JJ	denotes	genomic
T3840	15029-15032	NN	denotes	DNA
T3844	15033-15036	VBD	denotes	was
T3843	15037-15045	VBN	denotes	digested
T3845	15046-15051	VBG	denotes	using
T3846	15052-15057	NN	denotes	EcoRI
T3847	15057-15059	,	denotes	,
T3848	15059-15064	NN	denotes	BamHI
T3849	15064-15066	,	denotes	,
T3850	15066-15070	NN	denotes	PstI
T3851	15070-15072	,	denotes	,
T3852	15072-15075	CC	denotes	and
T3853	15076-15080	NN	denotes	XbaI
T3854	15080-15081	.	denotes	.
T3855	15081-15248	sentence	denotes	The digests were electrophoresed on a 1% agarose gel at 60V overnight and were transferred onto a Hybond N+ membrane from Amersham Pharmacia Biotech (Piscataway, NJ).
T3856	15082-15085	DT	denotes	The
T3857	15086-15093	NNS	denotes	digests
T3859	15094-15098	VBD	denotes	were
T3858	15099-15114	VBN	denotes	electrophoresed
T3860	15115-15117	IN	denotes	on
T3861	15118-15119	DT	denotes	a
T3863	15120-15121	CD	denotes	1
T3864	15121-15122	NN	denotes	%
T3865	15123-15130	NN	denotes	agarose
T3862	15131-15134	NN	denotes	gel
T3866	15135-15137	IN	denotes	at
T3867	15138-15141	NN	denotes	60V
T3868	15142-15151	RB	denotes	overnight
T3869	15152-15155	CC	denotes	and
T3870	15156-15160	VBD	denotes	were
T3871	15161-15172	VBN	denotes	transferred
T3872	15173-15177	IN	denotes	onto
T3873	15178-15179	DT	denotes	a
T3875	15180-15186	NN	denotes	Hybond
T3876	15187-15189	JJ	denotes	N+
T3874	15190-15198	NN	denotes	membrane
T3877	15199-15203	IN	denotes	from
T3878	15204-15212	NNP	denotes	Amersham
T3880	15213-15222	NNP	denotes	Pharmacia
T3879	15223-15230	NNP	denotes	Biotech
T3881	15231-15232	-LRB-	denotes	(
T3882	15232-15242	NNP	denotes	Piscataway
T3883	15242-15244	,	denotes	,
T3884	15244-15246	NNP	denotes	NJ
T3885	15246-15247	-RRB-	denotes	)
T3886	15247-15248	.	denotes	.
T3887	15248-15477	sentence	denotes	A 32P-dATP labeled PCR fragment of the Mcoln1 coding region corresponding to exon 2 was used as a probe (primers 5'-CCCCACAGAAGAGGAAGAC-3' (forward) and 5'-AGATCTTGACCACCTGCAG-3' (reverse) with an annealing temperature of 59°C).
T3888	15249-15250	DT	denotes	A
T3890	15251-15254	NN	denotes	32P
T3892	15254-15255	HYPH	denotes	-
T3891	15255-15259	NN	denotes	dATP
T3893	15260-15267	JJ	denotes	labeled
T3894	15268-15271	NN	denotes	PCR
T3889	15272-15280	NN	denotes	fragment
T3896	15281-15283	IN	denotes	of
T3897	15284-15287	DT	denotes	the
T3899	15288-15294	NN	denotes	Mcoln1
T3900	15295-15301	NN	denotes	coding
T3898	15302-15308	NN	denotes	region
T3901	15309-15322	VBG	denotes	corresponding
T3902	15323-15325	IN	denotes	to
T3903	15326-15330	NN	denotes	exon
T3904	15331-15332	CD	denotes	2
T3905	15333-15336	VBD	denotes	was
T3895	15337-15341	VBN	denotes	used
T3906	15342-15344	IN	denotes	as
T3907	15345-15346	DT	denotes	a
T3908	15347-15352	NN	denotes	probe
T3909	15353-15354	-LRB-	denotes	(
T3911	15354-15361	NNS	denotes	primers
T3912	15362-15363	CD	denotes	5
T3913	15363-15364	SYM	denotes	'
T3914	15364-15365	HYPH	denotes	-
T3915	15365-15384	NN	denotes	CCCCACAGAAGAGGAAGAC
T3916	15384-15385	HYPH	denotes	-
T3910	15385-15386	NN	denotes	3
T3917	15386-15387	SYM	denotes	'
T3918	15388-15389	-LRB-	denotes	(
T3919	15389-15396	RB	denotes	forward
T3920	15396-15397	-RRB-	denotes	)
T3921	15398-15401	CC	denotes	and
T3922	15402-15403	CD	denotes	5
T3924	15403-15404	SYM	denotes	'
T3925	15404-15405	HYPH	denotes	-
T3926	15405-15424	NN	denotes	AGATCTTGACCACCTGCAG
T3927	15424-15425	HYPH	denotes	-
T3923	15425-15426	NN	denotes	3
T3928	15426-15427	SYM	denotes	'
T3929	15428-15429	-LRB-	denotes	(
T3930	15429-15436	RB	denotes	reverse
T3931	15436-15437	-RRB-	denotes	)
T3932	15438-15442	IN	denotes	with
T3933	15443-15445	DT	denotes	an
T3935	15446-15455	JJ	denotes	annealing
T3934	15456-15467	NN	denotes	temperature
T3936	15468-15470	IN	denotes	of
T3937	15471-15473	CD	denotes	59
T3938	15473-15475	NNS	denotes	°C
T3939	15475-15476	-RRB-	denotes	)
T3940	15476-15477	.	denotes	.
T3941	15477-15548	sentence	denotes	Hybridization and washes were carried out in standard conditions [16].
T3942	15478-15491	NN	denotes	Hybridization
T3944	15492-15495	CC	denotes	and
T3945	15496-15502	NNS	denotes	washes
T3946	15503-15507	VBD	denotes	were
T3943	15508-15515	VBN	denotes	carried
T3947	15516-15519	RP	denotes	out
T3948	15520-15522	IN	denotes	in
T3949	15523-15531	JJ	denotes	standard
T3950	15532-15542	NNS	denotes	conditions
T3951	15543-15544	-LRB-	denotes	[
T3952	15544-15546	CD	denotes	16
T3953	15546-15547	-RRB-	denotes	]
T3954	15547-15548	.	denotes	.
T4011	15550-15558	NNP	denotes	Northern
T4012	15559-15563	NN	denotes	Blot
T4013	15564-15572	NN	denotes	Analysis
T4014	15572-15728	sentence	denotes	Mouse embryo multiple-tissue northern blot and mouse adult multiple-tissue northern blot filters were purchased from Clontech Laboratories (Palo Alto, CA).
T4015	15573-15578	NN	denotes	Mouse
T4017	15579-15585	NN	denotes	embryo
T4018	15586-15594	JJ	denotes	multiple
T4019	15594-15595	HYPH	denotes	-
T4016	15595-15601	NN	denotes	tissue
T4021	15602-15610	JJ	denotes	northern
T4020	15611-15615	NN	denotes	blot
T4023	15616-15619	CC	denotes	and
T4024	15620-15625	NN	denotes	mouse
T4026	15626-15631	JJ	denotes	adult
T4027	15632-15640	JJ	denotes	multiple
T4028	15640-15641	HYPH	denotes	-
T4025	15641-15647	NN	denotes	tissue
T4030	15648-15656	JJ	denotes	northern
T4029	15657-15661	NN	denotes	blot
T4022	15662-15669	NNS	denotes	filters
T4032	15670-15674	VBD	denotes	were
T4031	15675-15684	VBN	denotes	purchased
T4033	15685-15689	IN	denotes	from
T4034	15690-15698	NNP	denotes	Clontech
T4035	15699-15711	NNP	denotes	Laboratories
T4036	15712-15713	-LRB-	denotes	(
T4038	15713-15717	NNP	denotes	Palo
T4037	15718-15722	NNP	denotes	Alto
T4039	15722-15724	,	denotes	,
T4040	15724-15726	NNP	denotes	CA
T4041	15726-15727	-RRB-	denotes	)
T4042	15727-15728	.	denotes	.
T4043	15728-15856	sentence	denotes	The filters were hybridized with the 32P-dATP labeled DNA fragment of Mcoln1 coding region corresponding to exon 2 (see above).
T4044	15729-15732	DT	denotes	The
T4045	15733-15740	NNS	denotes	filters
T4047	15741-15745	VBD	denotes	were
T4046	15746-15756	VBN	denotes	hybridized
T4048	15757-15761	IN	denotes	with
T4049	15762-15765	DT	denotes	the
T4051	15766-15769	NN	denotes	32P
T4053	15769-15770	HYPH	denotes	-
T4052	15770-15774	NN	denotes	dATP
T4054	15775-15782	JJ	denotes	labeled
T4055	15783-15786	NN	denotes	DNA
T4050	15787-15795	NN	denotes	fragment
T4056	15796-15798	IN	denotes	of
T4057	15799-15805	NN	denotes	Mcoln1
T4059	15806-15812	NN	denotes	coding
T4058	15813-15819	NN	denotes	region
T4060	15820-15833	VBG	denotes	corresponding
T4061	15834-15836	IN	denotes	to
T4062	15837-15841	NN	denotes	exon
T4063	15842-15843	CD	denotes	2
T4064	15844-15845	-LRB-	denotes	(
T4065	15845-15848	VB	denotes	see
T4066	15849-15854	RB	denotes	above
T4067	15854-15855	-RRB-	denotes	)
T4068	15855-15856	.	denotes	.
T4069	15856-16076	sentence	denotes	For the alternative transcript, a probe was generated in the region between exons 12 and 13 with primers 5'-GTGTCCACCACCTGAGAG-3' (forward) and 5'-GAAGTAGCATTCCTGCAGGC-3' (reverse) with an annealing temperature of 62°C.
T4070	15857-15860	IN	denotes	For
T4072	15861-15864	DT	denotes	the
T4074	15865-15876	JJ	denotes	alternative
T4073	15877-15887	NN	denotes	transcript
T4075	15887-15889	,	denotes	,
T4076	15889-15890	DT	denotes	a
T4077	15891-15896	NN	denotes	probe
T4078	15897-15900	VBD	denotes	was
T4071	15901-15910	VBN	denotes	generated
T4079	15911-15913	IN	denotes	in
T4080	15914-15917	DT	denotes	the
T4081	15918-15924	NN	denotes	region
T4082	15925-15932	IN	denotes	between
T4083	15933-15938	NNS	denotes	exons
T4084	15939-15941	CD	denotes	12
T4085	15942-15945	CC	denotes	and
T4086	15946-15948	CD	denotes	13
T4087	15949-15953	IN	denotes	with
T4088	15954-15961	NNS	denotes	primers
T4090	15962-15963	NN	denotes	5
T4091	15963-15964	SYM	denotes	'
T4092	15964-15965	HYPH	denotes	-
T4093	15965-15983	NN	denotes	GTGTCCACCACCTGAGAG
T4094	15983-15984	HYPH	denotes	-
T4089	15984-15985	NN	denotes	3
T4095	15985-15986	SYM	denotes	'
T4096	15987-15988	-LRB-	denotes	(
T4097	15988-15995	RB	denotes	forward
T4098	15995-15996	-RRB-	denotes	)
T4099	15997-16000	CC	denotes	and
T4100	16001-16002	NN	denotes	5
T4102	16002-16003	SYM	denotes	'
T4103	16003-16004	HYPH	denotes	-
T4104	16004-16024	NN	denotes	GAAGTAGCATTCCTGCAGGC
T4105	16024-16025	HYPH	denotes	-
T4101	16025-16026	NN	denotes	3
T4106	16026-16027	SYM	denotes	'
T4107	16028-16029	-LRB-	denotes	(
T4108	16029-16036	RB	denotes	reverse
T4109	16036-16037	-RRB-	denotes	)
T4110	16038-16042	IN	denotes	with
T4111	16043-16045	DT	denotes	an
T4113	16046-16055	JJ	denotes	annealing
T4112	16056-16067	NN	denotes	temperature
T4114	16068-16070	IN	denotes	of
T4115	16071-16073	CD	denotes	62
T4116	16073-16075	NNS	denotes	°C
T4117	16075-16076	.	denotes	.
T4118	16076-16130	sentence	denotes	The filters were then hybridized with β-actin probes.
T4119	16077-16080	DT	denotes	The
T4120	16081-16088	NNS	denotes	filters
T4122	16089-16093	VBD	denotes	were
T4123	16094-16098	RB	denotes	then
T4121	16099-16109	VBN	denotes	hybridized
T4124	16110-16114	IN	denotes	with
T4125	16115-16116	NN	denotes	β
T4127	16116-16117	HYPH	denotes	-
T4126	16117-16122	NN	denotes	actin
T4128	16123-16129	NNS	denotes	probes
T4129	16129-16130	.	denotes	.
T4130	16130-16260	sentence	denotes	Hybridizations and washes were carried out in standard conditions, with the stripping of previously bound probes in between [16].
T4131	16131-16145	NNS	denotes	Hybridizations
T4133	16146-16149	CC	denotes	and
T4134	16150-16156	NNS	denotes	washes
T4135	16157-16161	VBD	denotes	were
T4132	16162-16169	VBN	denotes	carried
T4136	16170-16173	RP	denotes	out
T4137	16174-16176	IN	denotes	in
T4138	16177-16185	JJ	denotes	standard
T4139	16186-16196	NNS	denotes	conditions
T4140	16196-16198	,	denotes	,
T4141	16198-16202	IN	denotes	with
T4142	16203-16206	DT	denotes	the
T4143	16207-16216	NN	denotes	stripping
T4144	16217-16219	IN	denotes	of
T4145	16220-16230	RB	denotes	previously
T4146	16231-16236	VBN	denotes	bound
T4147	16237-16243	NNS	denotes	probes
T4148	16244-16246	RB	denotes	in
T4149	16247-16254	RB	denotes	between
T4150	16255-16256	-LRB-	denotes	[
T4151	16256-16258	CD	denotes	16
T4152	16258-16259	-RRB-	denotes	]
T4153	16259-16260	.	denotes	.
R10	T123	T122	compound	mouse,gene
R100	T226	T225	pobj	synteny,of
R1000	T1679	T1680	nmod	Mouse,tissue
R1001	T1680	T1683	nsubjpass	tissue,hybridized
R1002	T1681	T1680	amod	adult,tissue
R1003	T1682	T1680	amod	multiple,tissue
R1004	T1684	T1680	cc	and,tissue
R1005	T1685	T1686	amod	embryonic,blots
R1006	T1686	T1680	conj	blots,tissue
R1007	T1687	T1686	compound	Northern,blots
R1008	T1688	T1683	auxpass	were,hybridized
R1009	T1689	T1683	advcl	using,hybridized
R101	T227	T221	punct	.,display
R1010	T1690	T1691	det	a,probe
R1011	T1691	T1689	dobj	probe,using
R1012	T1692	T1691	acl	generated,probe
R1013	T1693	T1692	prep	from,generated
R1014	T1694	T1695	compound	mouse,exon
R1015	T1695	T1693	pobj	exon,from
R1016	T1696	T1695	nummod	2,exon
R1017	T1697	T1698	punct	(,1B
R1018	T1698	T1691	parataxis	1B,probe
R1019	T1699	T1698	dep	probe,1B
R102	T229	T230	nsubj	Mcoln1,shows
R1020	T1700	T1699	nummod	1,probe
R1021	T1701	T1698	punct	", ",1B
R1022	T1702	T1698	compound	Fig.,1B
R1023	T1703	T1698	punct	),1B
R1024	T1704	T1683	punct	", ",hybridized
R1025	T1705	T1683	advcl	yielding,hybridized
R1026	T1706	T1707	det	a,band
R1027	T1707	T1705	dobj	band,yielding
R1028	T1708	T1707	prep	of,band
R1029	T1709	T1710	advmod	approximately,2.4
R103	T231	T232	nummod	91,%
R1030	T1710	T1711	nummod	2.4,kb
R1031	T1711	T1708	pobj	kb,of
R1032	T1712	T1707	punct	(,band
R1033	T1713	T1707	appos	isoform,band
R1034	T1714	T1713	nummod	1,isoform
R1035	T1715	T1707	punct	),band
R1036	T1716	T1707	punct	", ",band
R1037	T1717	T1718	mark	as,expected
R1038	T1718	T1707	advcl	expected,band
R1039	T1719	T1707	punct	", ",band
R104	T232	T233	nmod	%,acid
R1040	T1720	T1707	cc	and,band
R1041	T1721	T1722	det	a,band
R1042	T1722	T1707	conj	band,band
R1043	T1723	T1724	advmod	less,abundant
R1044	T1724	T1722	amod	abundant,band
R1045	T1725	T1724	cc	and,abundant
R1046	T1726	T1724	conj	unexpected,abundant
R1047	T1727	T1728	nummod	4.4,kb
R1048	T1728	T1722	compound	kb,band
R1049	T1729	T1722	punct	(,band
R105	T233	T235	nmod	acid,identity
R1050	T1730	T1722	appos	isoform,band
R1051	T1731	T1730	nummod	2,isoform
R1052	T1732	T1705	punct	),yielding
R1053	T1733	T1734	punct	(,3A
R1054	T1734	T1705	parataxis	3A,yielding
R1055	T1735	T1734	compound	Figs.,3A
R1056	T1736	T1734	cc	&,3A
R1057	T1737	T1734	conj	3B,3A
R1058	T1738	T1734	punct	),3A
R1059	T1739	T1683	punct	.,hybridized
R106	T234	T233	nmod	amino,acid
R1060	T1741	T1742	det	The,band
R1061	T1742	T1745	nsubj	band,shows
R1062	T1743	T1744	nummod	2.4,kb
R1063	T1744	T1742	compound	kb,band
R1064	T1746	T1747	amod	ubiquitous,expression
R1065	T1747	T1745	dobj	expression,shows
R1066	T1748	T1746	cc	but,ubiquitous
R1067	T1749	T1746	conj	variable,ubiquitous
R1068	T1750	T1745	punct	", ",shows
R1069	T1751	T1752	mark	with,expression
R107	T235	T230	dobj	identity,shows
R1070	T1752	T1745	advcl	expression,shows
R1071	T1753	T1752	det	the,expression
R1072	T1754	T1752	amod	highest,expression
R1073	T1755	T1752	prep	in,expression
R1074	T1756	T1755	pobj	brain,in
R1075	T1757	T1756	punct	", ",brain
R1076	T1758	T1756	conj	liver,brain
R1077	T1759	T1758	cc	and,liver
R1078	T1760	T1758	conj	kidney,liver
R1079	T1761	T1745	punct	.,shows
R108	T236	T233	cc	and,acid
R1080	T1763	T1764	det	The,blot
R1081	T1764	T1767	nsubj	blot,shows
R1082	T1765	T1766	amod	fetal,tissue
R1083	T1766	T1764	compound	tissue,blot
R1084	T1768	T1769	amod	decreasing,levels
R1085	T1769	T1767	dobj	levels,shows
R1086	T1770	T1769	prep	of,levels
R1087	T1771	T1772	det	the,message
R1088	T1772	T1770	pobj	message,of
R1089	T1773	T1774	nummod	2.4,kb
R109	T237	T238	nummod	86,%
R1090	T1774	T1772	compound	kb,message
R1091	T1775	T1769	prep	with,levels
R1092	T1776	T1777	amod	increasing,age
R1093	T1777	T1775	pobj	age,with
R1094	T1778	T1777	amod	gestational,age
R1095	T1779	T1767	punct	.,shows
R1096	T1781	T1782	mark	Since,encodes
R1097	T1782	T1787	advcl	encodes,carried
R1098	T1783	T1784	det	the,gene
R1099	T1784	T1782	nsubj	gene,encodes
R11	T124	T122	compound	Mcoln1,gene
R110	T238	T239	compound	%,nucleotide
R1100	T1785	T1784	amod	human,gene
R1101	T1786	T1784	compound	MCOLN1,gene
R1102	T1788	T1789	det	a,transcript
R1103	T1789	T1782	dobj	transcript,encodes
R1104	T1790	T1789	amod	single,transcript
R1105	T1791	T1792	punct	[,7
R1106	T1792	T1782	parataxis	7,encodes
R1107	T1793	T1794	punct	-,9
R1108	T1794	T1792	prep	9,7
R1109	T1795	T1792	punct	],7
R111	T239	T233	conj	nucleotide,acid
R1110	T1796	T1787	punct	", ",carried
R1111	T1797	T1787	nsubj	we,carried
R1112	T1798	T1787	prt	out,carried
R1113	T1799	T1800	amod	additional,hybridizations
R1114	T1800	T1787	dobj	hybridizations,carried
R1115	T1801	T1787	prep	with,carried
R1116	T1802	T1801	pobj	probes,with
R1117	T1803	T1802	acl	generated,probes
R1118	T1804	T1803	prep	from,generated
R1119	T1805	T1806	amod	various,regions
R112	T240	T235	prep	to,identity
R1120	T1806	T1804	pobj	regions,from
R1121	T1807	T1806	prep	of,regions
R1122	T1808	T1809	det	the,sequence
R1123	T1809	T1807	pobj	sequence,of
R1124	T1810	T1809	amod	coding,sequence
R1125	T1811	T1809	cc	and,sequence
R1126	T1812	T1813	nummod	3,UTR
R1127	T1813	T1809	conj	UTR,sequence
R1128	T1814	T1812	punct	',3
R1129	T1815	T1787	punct	", ",carried
R113	T241	T240	pobj	MCOLN1,to
R1130	T1816	T1787	cc	and,carried
R1131	T1817	T1818	det	all,probes
R1132	T1818	T1819	nsubj	probes,identified
R1133	T1819	T1787	conj	identified,carried
R1134	T1820	T1821	det	the,transcripts
R1135	T1821	T1819	dobj	transcripts,identified
R1136	T1822	T1821	amod	same,transcripts
R1137	T1823	T1821	nummod	two,transcripts
R1138	T1824	T1819	prep	in,identified
R1139	T1825	T1826	det	the,mouse
R114	T242	T230	punct	.,shows
R1140	T1826	T1824	pobj	mouse,in
R1141	T1827	T1828	punct	(,shown
R1142	T1828	T1819	parataxis	shown,identified
R1143	T1829	T1828	nsubj	data,shown
R1144	T1830	T1828	neg	not,shown
R1145	T1831	T1828	punct	),shown
R1146	T1832	T1819	punct	.,identified
R1147	T1834	T1835	prep	In,hybridized
R1148	T1836	T1834	pobj	order,In
R1149	T1837	T1838	aux	to,verify
R115	T244	T245	advmod	Also,maps
R1150	T1838	T1836	acl	verify,order
R1151	T1839	T1840	det	the,presence
R1152	T1840	T1838	dobj	presence,verify
R1153	T1841	T1840	prep	of,presence
R1154	T1842	T1843	det	a,locus
R1155	T1843	T1841	pobj	locus,of
R1156	T1844	T1843	amod	single,locus
R1157	T1845	T1843	compound	mouse,locus
R1158	T1846	T1835	punct	", ",hybridized
R1159	T1847	T1835	nsubj	we,hybridized
R116	T246	T245	punct	", ",maps
R1160	T1848	T1849	det	a,blot
R1161	T1849	T1835	dobj	blot,hybridized
R1162	T1850	T1849	compound	mouse,blot
R1163	T1851	T1849	compound	Southern,blot
R1164	T1852	T1835	prep	with,hybridized
R1165	T1853	T1854	det	the,probe
R1166	T1854	T1852	pobj	probe,with
R1167	T1855	T1854	nmod	exon,probe
R1168	T1856	T1855	nummod	2,exon
R1169	T1857	T1835	punct	.,hybridized
R117	T247	T245	nsubj	Mcoln1,maps
R1170	T1859	T1860	nummod	Four,enzymes
R1171	T1860	T1863	nsubjpass	enzymes,used
R1172	T1861	T1860	amod	different,enzymes
R1173	T1862	T1860	compound	restriction,enzymes
R1174	T1864	T1863	auxpass	were,used
R1175	T1865	T1863	punct	", ",used
R1176	T1866	T1863	cc	and,used
R1177	T1867	T1868	advmod	only,bands
R1178	T1868	T1872	nsubjpass	bands,detected
R1179	T1869	T1868	det	the,bands
R118	T248	T245	prep	to,maps
R1180	T1870	T1871	amod	expected,size
R1181	T1871	T1868	compound	size,bands
R1182	T1872	T1863	conj	detected,used
R1183	T1873	T1868	prep	for,bands
R1184	T1874	T1875	det	the,locus
R1185	T1875	T1873	pobj	locus,for
R1186	T1876	T1875	nmod	chromosome,locus
R1187	T1877	T1876	nummod	8,chromosome
R1188	T1878	T1872	auxpass	were,detected
R1189	T1879	T1880	punct	(,shown
R119	T249	T248	pobj	chromosome,to
R1190	T1880	T1872	parataxis	shown,detected
R1191	T1881	T1880	nsubj	data,shown
R1192	T1882	T1880	neg	not,shown
R1193	T1883	T1880	punct	),shown
R1194	T1884	T1872	punct	.,detected
R12	T125	T126	det	an,transcript
R120	T250	T249	nummod	8,chromosome
R1200	T2106	T2105	prep	of,Characterization
R1201	T2107	T2108	det	the,variant
R1202	T2108	T2106	pobj	variant,of
R1203	T2109	T2108	nmod	Mcoln1,variant
R1204	T2110	T2111	amod	alternative,splice
R1205	T2111	T2108	compound	splice,variant
R1206	T2113	T2114	prep	In,searched
R1207	T2115	T2113	pobj	order,In
R1208	T2116	T2117	aux	to,determine
R1209	T2117	T2115	acl	determine,order
R121	T251	T245	cc	and,maps
R1210	T2118	T2119	det	the,sequence
R1211	T2119	T2117	dobj	sequence,determine
R1212	T2120	T2119	amod	coding,sequence
R1213	T2121	T2119	prep	for,sequence
R1214	T2122	T2123	det	the,transcript
R1215	T2123	T2121	pobj	transcript,for
R1216	T2124	T2123	amod	larger,transcript
R1217	T2125	T2114	punct	", ",searched
R1218	T2126	T2114	nsubj	we,searched
R1219	T2127	T2128	det	the,database
R122	T252	T245	conj	contains,maps
R1220	T2128	T2114	dobj	database,searched
R1221	T2129	T2130	compound	mouse,EST
R1222	T2130	T2128	compound	EST,database
R1223	T2131	T2114	advcl	using,searched
R1224	T2132	T2133	det	each,intron
R1225	T2133	T2131	dobj	intron,using
R1226	T2134	T2135	advmod	as,as
R1227	T2135	T2133	cc	as,intron
R1228	T2136	T2135	advmod	well,as
R1229	T2137	T2138	det	the,sequence
R123	T253	T254	det	an,frame
R1230	T2138	T2133	conj	sequence,intron
R1231	T2139	T2138	amod	genomic,sequence
R1232	T2140	T2138	acl	flanking,sequence
R1233	T2141	T2142	det	the,gene
R1234	T2142	T2140	dobj	gene,flanking
R1235	T2143	T2142	compound	Mcoln1,gene
R1236	T2144	T2114	punct	.,searched
R1237	T2146	T2147	nummod	Two,ESTs
R1238	T2147	T2148	nsubjpass	ESTs,identified
R1239	T2149	T2148	auxpass	were,identified
R124	T254	T252	dobj	frame,contains
R1240	T2150	T2151	dep	that,contained
R1241	T2151	T2148	ccomp	contained,identified
R1242	T2152	T2151	dobj	sequence,contained
R1243	T2153	T2152	prep	from,sequence
R1244	T2154	T2153	pobj	intron,from
R1245	T2155	T2154	nummod	12,intron
R1246	T2156	T2157	punct	(,AI430291
R1247	T2157	T2148	parataxis	AI430291,identified
R1248	T2158	T2157	compound	GB,AI430291
R1249	T2159	T2157	compound	No.,AI430291
R125	T255	T254	amod	open,frame
R1250	T2160	T2157	cc	and,AI430291
R1251	T2161	T2157	conj	AA874645,AI430291
R1252	T2162	T2157	punct	),AI430291
R1253	T2163	T2148	punct	", ",identified
R1254	T2164	T2148	cc	and,identified
R1255	T2165	T2166	det	the,clones
R1256	T2166	T2168	nsubjpass	clones,sequenced
R1257	T2167	T2166	amod	corresponding,clones
R1258	T2168	T2148	conj	sequenced,identified
R1259	T2169	T2168	auxpass	were,sequenced
R126	T256	T254	compound	reading,frame
R1260	T2170	T2168	punct	.,sequenced
R1261	T2172	T2173	nsubj	Clone,begins
R1262	T2174	T2172	nummod	408619,Clone
R1263	T2175	T2176	punct	(,AI429558
R1264	T2176	T2172	parataxis	AI429558,Clone
R1265	T2177	T2176	dep	ESTs,AI429558
R1266	T2178	T2176	punct	: ,AI429558
R1267	T2179	T2176	nmod	GB,AI429558
R1268	T2180	T2176	nmod	No.,AI429558
R1269	T2181	T2176	nmod	AI430291,AI429558
R127	T257	T254	prep	of,frame
R1270	T2182	T2176	punct	", ",AI429558
R1271	T2183	T2176	punct	),AI429558
R1272	T2184	T2185	advmod	approximately,1.1
R1273	T2185	T2186	nummod	1.1,kb
R1274	T2186	T2187	npadvmod	kb,before
R1275	T2187	T2173	prep	before,begins
R1276	T2188	T2187	pobj	exon,before
R1277	T2189	T2188	nummod	13,exon
R1278	T2190	T2173	cc	and,begins
R1279	T2191	T2173	conj	continues,begins
R128	T258	T259	nummod	580,acids
R1280	T2192	T2191	prep	through,continues
R1281	T2193	T2194	det	the,exon
R1282	T2194	T2192	pobj	exon,through
R1283	T2195	T2191	cc	and,continues
R1284	T2196	T2191	conj	splices,continues
R1285	T2197	T2196	advmod	correctly,splices
R1286	T2198	T2196	prep	to,splices
R1287	T2199	T2198	pobj	exon,to
R1288	T2200	T2199	nummod	14,exon
R1289	T2201	T2173	punct	.,begins
R129	T259	T257	pobj	acids,of
R1290	T2203	T2204	nsubj	Clone,begins
R1291	T2205	T2203	nummod	1281641,Clone
R1292	T2206	T2207	punct	(,AA874645
R1293	T2207	T2203	parataxis	AA874645,Clone
R1294	T2208	T2207	dep	EST,AA874645
R1295	T2209	T2207	punct	:,AA874645
R1296	T2210	T2207	compound	GB,AA874645
R1297	T2211	T2207	compound	No.,AA874645
R1298	T2212	T2207	punct	),AA874645
R1299	T2213	T2214	nummod	175,bp
R13	T126	T116	dobj	transcript,reveals
R130	T260	T259	compound	amino,acids
R1300	T2214	T2215	npadvmod	bp,before
R1301	T2215	T2204	prep	before,begins
R1302	T2216	T2215	pobj	exon,before
R1303	T2217	T2216	nummod	13,exon
R1304	T2218	T2204	cc	and,begins
R1305	T2219	T2220	advmod	also,splices
R1306	T2220	T2204	conj	splices,begins
R1307	T2221	T2220	advmod	correctly,splices
R1308	T2222	T2220	prep	to,splices
R1309	T2223	T2222	pobj	exon,to
R131	T261	T254	punct	", ",frame
R1310	T2224	T2223	nummod	14,exon
R1311	T2225	T2204	punct	.,begins
R1312	T2227	T2228	det	A,Northern
R1313	T2228	T2232	nsubjpass	Northern,hybridized
R1314	T2229	T2228	nmod	mouse,Northern
R1315	T2230	T2231	amod	multiple,tissue
R1316	T2231	T2228	compound	tissue,Northern
R1317	T2233	T2232	auxpass	was,hybridized
R1318	T2234	T2232	advcl	using,hybridized
R1319	T2235	T2236	det	a,probe
R132	T262	T254	prep	with,frame
R1320	T2236	T2234	dobj	probe,using
R1321	T2237	T2236	acl	generated,probe
R1322	T2238	T2237	prep	from,generated
R1323	T2239	T2240	det	the,sequence
R1324	T2240	T2238	pobj	sequence,from
R1325	T2241	T2240	amod	putative,sequence
R1326	T2242	T2240	compound	intron,sequence
R1327	T2243	T2240	prep	in,sequence
R1328	T2244	T2243	pobj	clone,in
R1329	T2245	T2244	nummod	408619,clone
R133	T263	T264	det	a,length
R1330	T2246	T2247	punct	(,1B
R1331	T2247	T2236	parataxis	1B,probe
R1332	T2248	T2247	dep	Probe,1B
R1333	T2249	T2248	nummod	2,Probe
R1334	T2250	T2247	punct	", ",1B
R1335	T2251	T2247	compound	Fig.,1B
R1336	T2252	T2247	punct	),1B
R1337	T2253	T2236	punct	", ",probe
R1338	T2254	T2255	dep	which,detected
R1339	T2255	T2236	relcl	detected,probe
R134	T264	T262	pobj	length,with
R1340	T2256	T2257	advmod	only,band
R1341	T2257	T2255	dobj	band,detected
R1342	T2258	T2257	det	the,band
R1343	T2259	T2260	nummod	4.4,kb
R1344	T2260	T2257	compound	kb,band
R1345	T2261	T2262	punct	(,3C
R1346	T2262	T2255	parataxis	3C,detected
R1347	T2263	T2262	compound	Fig.,3C
R1348	T2264	T2262	punct	),3C
R1349	T2265	T2232	punct	.,hybridized
R135	T265	T264	compound	transcript,length
R1350	T2267	T2268	prep	In,performed
R1351	T2269	T2267	pobj	order,In
R1352	T2270	T2271	aux	to,determine
R1353	T2271	T2269	acl	determine,order
R1354	T2272	T2273	det	the,sequence
R1355	T2273	T2271	dobj	sequence,determine
R1356	T2274	T2273	prep	of,sequence
R1357	T2275	T2276	det	the,transcript
R1358	T2276	T2274	pobj	transcript,of
R1359	T2277	T2276	amod	entire,transcript
R136	T266	T264	prep	of,length
R1360	T2278	T2268	punct	", ",performed
R1361	T2279	T2280	compound	RT,PCR
R1362	T2280	T2268	nsubjpass	PCR,performed
R1363	T2281	T2280	punct	-,PCR
R1364	T2282	T2280	acl	using,PCR
R1365	T2283	T2282	dobj	primers,using
R1366	T2284	T2283	prep	in,primers
R1367	T2285	T2286	nmod	exons,10
R1368	T2286	T2284	pobj	10,in
R1369	T2287	T2286	cc	and,10
R137	T267	T268	advmod	approximately,2
R1370	T2288	T2286	conj	11,10
R1371	T2289	T2283	acl	paired,primers
R1372	T2290	T2289	prep	with,paired
R1373	T2291	T2292	det	a,primer
R1374	T2292	T2290	pobj	primer,with
R1375	T2293	T2292	prep	in,primer
R1376	T2294	T2293	pobj	intron,in
R1377	T2295	T2294	nummod	12,intron
R1378	T2296	T2268	auxpass	was,performed
R1379	T2297	T2268	advcl	using,performed
R138	T268	T269	nummod	2,kb
R1380	T2298	T2299	nmod	BALB,c
R1381	T2299	T2301	nmod	c,mouse
R1382	T2300	T2299	punct	/,c
R1383	T2301	T2302	nmod	mouse,brain
R1384	T2302	T2303	nmod	brain,RNA
R1385	T2303	T2297	dobj	RNA,using
R1386	T2304	T2303	amod	total,RNA
R1387	T2305	T2268	cc	and,performed
R1388	T2306	T2307	det	the,products
R1389	T2307	T2309	nsubj	products,sequenced
R139	T269	T266	pobj	kb,of
R1390	T2308	T2307	amod	resulting,products
R1391	T2309	T2268	conj	sequenced,performed
R1392	T2310	T2268	punct	.,performed
R1393	T2312	T2313	det	These,products
R1394	T2313	T2314	nsubj	products,show
R1395	T2315	T2316	mark	that,is
R1396	T2316	T2314	ccomp	is,show
R1397	T2317	T2318	det	the,transcript
R1398	T2318	T2316	nsubj	transcript,is
R1399	T2319	T2318	amod	larger,transcript
R14	T127	T128	advmod	alternatively,spliced
R140	T270	T264	acl	encoded,length
R1400	T2320	T2316	prep	due,is
R1401	T2321	T2320	pcomp	to,due
R1402	T2322	T2323	det	an,event
R1403	T2323	T2320	pobj	event,due
R1404	T2324	T2323	amod	alternative,event
R1405	T2325	T2323	compound	splice,event
R1406	T2326	T2327	dep	that,results
R1407	T2327	T2323	relcl	results,event
R1408	T2328	T2327	prep	in,results
R1409	T2329	T2330	det	an,exon
R141	T271	T270	agent	by,encoded
R1410	T2330	T2328	pobj	exon,in
R1411	T2331	T2330	amod	expanded,exon
R1412	T2332	T2330	nummod	13,exon
R1413	T2333	T2314	punct	.,show
R1414	T2335	T2336	advmod	Specifically,splices
R1415	T2337	T2336	punct	", ",splices
R1416	T2338	T2336	nsubj	exon,splices
R1417	T2339	T2338	nummod	12,exon
R1418	T2340	T2336	prep	at,splices
R1419	T2341	T2340	pobj	bp,at
R142	T272	T273	nummod	14,exons
R1420	T2342	T2341	nummod	436,bp
R1421	T2343	T2341	prep	of,bp
R1422	T2344	T2343	pobj	intron,of
R1423	T2345	T2344	nummod	12,intron
R1424	T2346	T2336	punct	", ",splices
R1425	T2347	T2336	advcl	creating,splices
R1426	T2348	T2349	det	a,exon
R1427	T2349	T2347	dobj	exon,creating
R1428	T2350	T2349	amod	large,exon
R1429	T2351	T2352	nummod	1614,bp
R143	T273	T271	pobj	exons,by
R1430	T2352	T2349	compound	bp,exon
R1431	T2353	T2349	nummod	13,exon
R1432	T2354	T2355	dep	that,splices
R1433	T2355	T2349	relcl	splices,exon
R1434	T2356	T2355	advmod	correctly,splices
R1435	T2357	T2355	prep	to,splices
R1436	T2358	T2357	pobj	exon,to
R1437	T2359	T2358	nummod	14,exon
R1438	T2360	T2336	punct	.,splices
R1439	T2362	T2363	det	The,frame
R144	T274	T254	punct	", ",frame
R1440	T2363	T2366	nsubj	frame,is
R1441	T2364	T2363	amod	open,frame
R1442	T2365	T2363	compound	reading,frame
R1443	T2367	T2363	prep	of,frame
R1444	T2368	T2369	det	this,transcript
R1445	T2369	T2367	pobj	transcript,of
R1446	T2370	T2371	advmod	alternatively,spliced
R1447	T2371	T2369	amod	spliced,transcript
R1448	T2372	T2373	nummod	611,acids
R1449	T2373	T2366	attr	acids,is
R145	T275	T254	amod	similar,frame
R1450	T2374	T2373	compound	amino,acids
R1451	T2375	T2373	punct	", ",acids
R1452	T2376	T2377	nummod	28,acids
R1453	T2377	T2379	npadvmod	acids,longer
R1454	T2378	T2377	amod	amino,acids
R1455	T2379	T2373	amod	longer,acids
R1456	T2380	T2379	prep	than,longer
R1457	T2381	T2382	det	the,message
R1458	T2382	T2380	pobj	message,than
R1459	T2383	T2382	acl	encoded,message
R146	T276	T275	prep	to,similar
R1460	T2384	T2383	agent	by,encoded
R1461	T2385	T2386	det	the,transcript
R1462	T2386	T2384	pobj	transcript,by
R1463	T2387	T2388	nummod	2.4,kb
R1464	T2388	T2386	compound	kb,transcript
R1465	T2389	T2366	punct	.,is
R1466	T2391	T2392	amod	TMPred,analysis
R1467	T2392	T2393	nsubj	analysis,predicts
R1468	T2393	T2394	ccomp	predicts,diverge
R1469	T2395	T2396	mark	that,encodes
R147	T277	T278	poss	its,counterpart
R1470	T2396	T2393	ccomp	encodes,predicts
R1471	T2397	T2396	nsubj	isoform,encodes
R1472	T2398	T2397	nummod	2,isoform
R1473	T2399	T2400	det	a,protein
R1474	T2400	T2396	dobj	protein,encodes
R1475	T2401	T2400	amod	identical,protein
R1476	T2402	T2401	prep	in,identical
R1477	T2403	T2402	pobj	structure,in
R1478	T2404	T2401	prep	to,identical
R1479	T2405	T2404	pobj	Mcoln1,to
R148	T278	T276	pobj	counterpart,to
R1480	T2406	T2400	punct	", ",protein
R1481	T2407	T2400	acl	possessing,protein
R1482	T2408	T2409	nummod	6,domains
R1483	T2409	T2407	dobj	domains,possessing
R1484	T2410	T2409	compound	transmembrane,domains
R1485	T2411	T2409	cc	and,domains
R1486	T2412	T2413	det	a,pore
R1487	T2413	T2409	conj	pore,domains
R1488	T2414	T2413	compound	channel,pore
R1489	T2415	T2394	punct	", ",diverge
R149	T279	T278	amod	human,counterpart
R1490	T2416	T2394	advmod	however,diverge
R1491	T2417	T2418	det	the,sequences
R1492	T2418	T2394	nsubj	sequences,diverge
R1493	T2419	T2418	compound	protein,sequences
R1494	T2420	T2394	prep	at,diverge
R1495	T2421	T2422	amod	amino,acid
R1496	T2422	T2420	pobj	acid,at
R1497	T2423	T2422	nummod	526,acid
R1498	T2424	T2394	punct	.,diverge
R1499	T2426	T2427	det	The,tail
R15	T128	T126	amod	spliced,transcript
R150	T280	T245	punct	.,maps
R1500	T2427	T2435	nsubj	tail,is
R1501	T2428	T2429	nummod	55,acid
R1502	T2429	T2427	nmod	acid,tail
R1503	T2430	T2429	nmod	amino,acid
R1504	T2431	T2432	npadvmod	C,terminal
R1505	T2432	T2427	amod	terminal,tail
R1506	T2433	T2432	punct	-,terminal
R1507	T2434	T2427	amod	cytoplasmic,tail
R1508	T2436	T2427	acl	encoded,tail
R1509	T2437	T2436	agent	by,encoded
R151	T282	T283	det	The,transcript
R1510	T2438	T2439	det	the,transcript
R1511	T2439	T2437	pobj	transcript,by
R1512	T2440	T2441	nummod	2.4,kb
R1513	T2441	T2439	compound	kb,transcript
R1514	T2442	T2443	advmod	completely,different
R1515	T2443	T2435	acomp	different,is
R1516	T2444	T2443	prep	from,different
R1517	T2445	T2446	det	the,tail
R1518	T2446	T2444	pobj	tail,from
R1519	T2447	T2448	nummod	86,acid
R152	T283	T284	nsubj	transcript,encodes
R1520	T2448	T2446	compound	acid,tail
R1521	T2449	T2448	compound	amino,acid
R1522	T2450	T2446	acl	encoded,tail
R1523	T2451	T2450	agent	by,encoded
R1524	T2452	T2453	det	the,transcript
R1525	T2453	T2451	pobj	transcript,by
R1526	T2454	T2455	npadvmod	murine,specific
R1527	T2455	T2453	amod	specific,transcript
R1528	T2456	T2457	nummod	4.4,kb
R1529	T2457	T2453	compound	kb,transcript
R153	T285	T286	dep	that,results
R1530	T2458	T2459	punct	(,Fig.
R1531	T2459	T2435	parataxis	Fig.,is
R1532	T2460	T2459	nummod	4,Fig.
R1533	T2461	T2459	punct	),Fig.
R1534	T2462	T2435	punct	.,is
R1535	T2464	T2465	compound	Clontech,Blots
R1536	T2465	T2469	nsubjpass	Blots,hybridized
R1537	T2466	T2467	compound	Mouse,RNA
R1538	T2467	T2465	compound	RNA,Blots
R1539	T2468	T2465	compound	Master,Blots
R154	T286	T283	relcl	results,transcript
R1540	T2469	T2471	ccomp	hybridized,was
R1541	T2470	T2469	auxpass	were,hybridized
R1542	T2472	T2469	prep	with,hybridized
R1543	T2473	T2474	det	the,probes
R1544	T2474	T2472	pobj	probes,with
R1545	T2475	T2474	nmod	exon,probes
R1546	T2476	T2475	nummod	2,exon
R1547	T2477	T2475	cc	and,exon
R1548	T2478	T2475	conj	intron,exon
R1549	T2479	T2478	nummod	12,intron
R155	T287	T286	prep	from,results
R1550	T2480	T2474	acl	mentioned,probes
R1551	T2481	T2480	advmod	above,mentioned
R1552	T2482	T2469	prep	in,hybridized
R1553	T2483	T2484	det	an,attempt
R1554	T2484	T2482	pobj	attempt,in
R1555	T2485	T2486	aux	to,determine
R1556	T2486	T2484	acl	determine,attempt
R1557	T2487	T2488	mark	if,showed
R1558	T2488	T2486	ccomp	showed,determine
R1559	T2489	T2490	det	these,transcripts
R156	T288	T289	npadvmod	murine,specific
R1560	T2490	T2488	nsubj	transcripts,showed
R1561	T2491	T2490	nummod	two,transcripts
R1562	T2492	T2488	dobj	differences,showed
R1563	T2493	T2492	prep	in,differences
R1564	T2494	T2495	compound	expression,patterns
R1565	T2495	T2493	pobj	patterns,in
R1566	T2496	T2471	punct	", ",was
R1567	T2497	T2471	advmod	however,was
R1568	T2498	T2471	punct	", ",was
R1569	T2499	T2471	expl	there,was
R157	T289	T290	amod	specific,splicing
R1570	T2500	T2501	det	no,difference
R1571	T2501	T2471	attr	difference,was
R1572	T2502	T2501	amod	significant,difference
R1573	T2503	T2471	prep	in,was
R1574	T2504	T2505	det	the,tissues
R1575	T2505	T2503	pobj	tissues,in
R1576	T2506	T2505	nummod	22,tissues
R1577	T2507	T2505	acl	represented,tissues
R1578	T2508	T2509	punct	(,shown
R1579	T2509	T2471	parataxis	shown,was
R158	T290	T287	pobj	splicing,from
R1580	T2510	T2509	nsubj	data,shown
R1581	T2511	T2509	neg	not,shown
R1582	T2512	T2509	punct	),shown
R1583	T2513	T2471	punct	.,was
R1584	T2515	T2516	advmod	Next,compared
R1585	T2517	T2516	punct	", ",compared
R1586	T2518	T2516	nsubj	we,compared
R1587	T2519	T2516	advmod	directly,compared
R1588	T2520	T2521	det	the,sequence
R1589	T2521	T2516	dobj	sequence,compared
R159	T291	T290	amod	alternative,splicing
R1590	T2522	T2521	nmod	nucleotide,sequence
R1591	T2523	T2522	cc	and,nucleotide
R1592	T2524	T2525	compound	amino,acid
R1593	T2525	T2522	conj	acid,nucleotide
R1594	T2526	T2521	prep	of,sequence
R1595	T2527	T2528	det	the,transcript
R1596	T2528	T2526	pobj	transcript,of
R1597	T2529	T2530	advmod	alternatively,spliced
R1598	T2530	T2528	amod	spliced,transcript
R1599	T2531	T2528	compound	mouse,transcript
R16	T129	T130	neg	not,seen
R160	T292	T293	det	a,protein
R1600	T2532	T2516	prep	to,compared
R1601	T2533	T2534	det	the,sequence
R1602	T2534	T2532	pobj	sequence,to
R1603	T2535	T2534	amod	entire,sequence
R1604	T2536	T2537	amod	human,MCOLN1
R1605	T2537	T2534	nmod	MCOLN1,sequence
R1606	T2538	T2534	amod	genomic,sequence
R1607	T2539	T2516	cc	and,compared
R1608	T2540	T2516	conj	found,compared
R1609	T2541	T2542	det	no,similarity
R161	T293	T284	dobj	protein,encodes
R1610	T2542	T2540	dobj	similarity,found
R1611	T2543	T2542	amod	significant,similarity
R1612	T2544	T2516	punct	.,compared
R1613	T2546	T2547	mark	As,mentioned
R1614	T2547	T2548	advcl	mentioned,show
R1615	T2549	T2547	advmod	previously,mentioned
R1616	T2550	T2548	punct	", ",show
R1617	T2551	T2552	compound	Northern,blots
R1618	T2552	T2548	nsubj	blots,show
R1619	T2553	T2552	acl	performed,blots
R162	T294	T295	nummod	611,acid
R1620	T2554	T2553	prep	with,performed
R1621	T2555	T2556	amod	human,MCOLN1
R1622	T2556	T2557	compound	MCOLN1,probes
R1623	T2557	T2554	pobj	probes,with
R1624	T2558	T2559	advmod	only,transcript
R1625	T2559	T2548	dobj	transcript,show
R1626	T2560	T2559	nummod	one,transcript
R1627	T2561	T2562	nummod	2.4,kb
R1628	T2562	T2559	compound	kb,transcript
R1629	T2563	T2548	punct	.,show
R163	T295	T293	compound	acid,protein
R1630	T2565	T2566	prep	In,hybridized
R1631	T2567	T2565	pobj	addition,In
R1632	T2568	T2566	punct	", ",hybridized
R1633	T2569	T2566	nsubj	we,hybridized
R1634	T2570	T2571	det	a,Northern
R1635	T2571	T2566	dobj	Northern,hybridized
R1636	T2572	T2571	amod	human,Northern
R1637	T2573	T2574	amod	multiple,tissue
R1638	T2574	T2571	compound	tissue,Northern
R1639	T2575	T2571	cc	and,Northern
R164	T296	T295	compound	amino,acid
R1640	T2576	T2577	amod	human,Southern
R1641	T2577	T2571	conj	Southern,Northern
R1642	T2578	T2566	prep	with,hybridized
R1643	T2579	T2580	det	a,probe
R1644	T2580	T2578	pobj	probe,with
R1645	T2581	T2580	prep	in,probe
R1646	T2582	T2583	amod	human,intron
R1647	T2583	T2581	pobj	intron,in
R1648	T2584	T2583	nummod	12,intron
R1649	T2585	T2586	dep	that,is
R165	T297	T298	dep	that,differs
R1650	T2586	T2580	relcl	is,probe
R1651	T2587	T2586	acomp	adjacent,is
R1652	T2588	T2587	prep	to,adjacent
R1653	T2589	T2588	pobj	exon,to
R1654	T2590	T2589	nummod	13,exon
R1655	T2591	T2566	punct	.,hybridized
R1656	T2593	T2594	det	The,probe
R1657	T2594	T2595	nsubjpass	probe,located
R1658	T2596	T2595	auxpass	was,located
R1659	T2597	T2595	prep	in,located
R166	T298	T293	relcl	differs,protein
R1660	T2598	T2599	det	the,region
R1661	T2599	T2597	pobj	region,in
R1662	T2600	T2599	amod	syntenic,region
R1663	T2601	T2600	prep	to,syntenic
R1664	T2602	T2601	pobj	that,to
R1665	T2603	T2604	dep	which,encodes
R1666	T2604	T2602	relcl	encodes,that
R1667	T2605	T2606	det	the,transcript
R1668	T2606	T2604	dobj	transcript,encodes
R1669	T2607	T2606	amod	alternate,transcript
R167	T299	T298	prep	at,differs
R1670	T2608	T2606	compound	mouse,transcript
R1671	T2609	T2595	punct	.,located
R1672	T2611	T2612	advmod	Only,bands
R1673	T2612	T2615	nsubjpass	bands,detected
R1674	T2613	T2612	det	the,bands
R1675	T2614	T2612	amod	expected,bands
R1676	T2616	T2615	auxpass	were,detected
R1677	T2617	T2615	prep	on,detected
R1678	T2618	T2619	det	the,Southern
R1679	T2619	T2617	pobj	Southern,on
R168	T300	T301	det	the,terminus
R1680	T2620	T2615	cc	and,detected
R1681	T2621	T2622	det	no,bands
R1682	T2622	T2623	nsubjpass	bands,detected
R1683	T2623	T2615	conj	detected,detected
R1684	T2624	T2623	auxpass	were,detected
R1685	T2625	T2623	prep	on,detected
R1686	T2626	T2627	det	the,Northern
R1687	T2627	T2625	pobj	Northern,on
R1688	T2628	T2615	punct	", ",detected
R1689	T2629	T2615	advcl	confirming,detected
R169	T301	T299	pobj	terminus,at
R1690	T2630	T2631	mark	that,is
R1691	T2631	T2629	ccomp	is,confirming
R1692	T2632	T2633	det	this,transcript
R1693	T2633	T2631	nsubj	transcript,is
R1694	T2634	T2633	amod	alternative,transcript
R1695	T2635	T2631	acomp	specific,is
R1696	T2636	T2635	prep	to,specific
R1697	T2637	T2638	amod	murine,Mcoln1
R1698	T2638	T2636	pobj	Mcoln1,to
R1699	T2639	T2615	punct	.,detected
R17	T130	T126	acl	seen,transcript
R170	T302	T301	compound	c,terminus
R1700	T2641	T2642	amod	Recent,analysis
R1701	T2642	T2644	nsubj	analysis,yields
R1702	T2643	T2642	compound	BLASTP,analysis
R1703	T2645	T2642	prep	of,analysis
R1704	T2646	T2647	det	the,transcript
R1705	T2647	T2645	pobj	transcript,of
R1706	T2648	T2647	amod	alternate,transcript
R1707	T2649	T2647	compound	Mcoln1,transcript
R1708	T2650	T2651	det	a,match
R1709	T2651	T2644	dobj	match,yields
R171	T303	T301	punct	-,terminus
R1710	T2652	T2651	prep	to,match
R1711	T2653	T2654	det	a,protein
R1712	T2654	T2652	pobj	protein,to
R1713	T2655	T2654	amod	putative,protein
R1714	T2656	T2657	nummod	145,acid
R1715	T2657	T2654	nmod	acid,protein
R1716	T2658	T2657	nmod	amino,acid
R1717	T2659	T2654	amod	anonymous,protein
R1718	T2660	T2661	punct	(,BAB25862
R1719	T2661	T2654	parataxis	BAB25862,protein
R172	T304	T284	punct	.,encodes
R1720	T2662	T2661	compound	GB,BAB25862
R1721	T2663	T2661	compound	No.,BAB25862
R1722	T2664	T2661	punct	),BAB25862
R1723	T2665	T2654	acl	predicted,protein
R1724	T2666	T2665	prep	from,predicted
R1725	T2667	T2668	det	a,clone
R1726	T2668	T2666	pobj	clone,from
R1727	T2669	T2668	compound	RIKEN,clone
R1728	T2670	T2644	punct	.,yields
R1729	T2672	T2673	nsubj	It,is
R173	T308	T309	nsubj	Mcoln1,is
R1730	T2674	T2673	acomp	obvious,is
R1731	T2675	T2673	prep	from,is
R1732	T2676	T2677	poss	our,results
R1733	T2677	T2675	pobj	results,from
R1734	T2678	T2673	punct	", ",is
R1735	T2679	T2673	advmod	however,is
R1736	T2680	T2673	punct	", ",is
R1737	T2681	T2682	mark	that,is
R1738	T2682	T2673	ccomp	is,is
R1739	T2683	T2684	det	the,identification
R174	T310	T311	advmod	highly,similar
R1740	T2684	T2682	nsubj	identification,is
R1741	T2685	T2684	prep	of,identification
R1742	T2686	T2687	det	this,sequence
R1743	T2687	T2685	pobj	sequence,of
R1744	T2688	T2684	prep	as,identification
R1745	T2689	T2690	det	a,protein
R1746	T2690	T2688	pobj	protein,as
R1747	T2691	T2692	amod	full,length
R1748	T2692	T2690	compound	length,protein
R1749	T2693	T2692	punct	-,length
R175	T311	T309	acomp	similar,is
R1750	T2694	T2682	acomp	incorrect,is
R1751	T2695	T2696	mark	since,identify
R1752	T2696	T2682	advcl	identify,is
R1753	T2697	T2696	nsubj	probes,identify
R1754	T2698	T2697	amod	unique,probes
R1755	T2699	T2698	prep	to,unique
R1756	T2700	T2701	det	the,clone
R1757	T2701	T2699	pobj	clone,to
R1758	T2702	T2697	punct	", ",probes
R1759	T2703	T2704	advmod	as,as
R176	T312	T311	prep	to,similar
R1760	T2704	T2697	cc	as,probes
R1761	T2705	T2704	advmod	well,as
R1762	T2706	T2697	conj	probes,probes
R1763	T2707	T2706	acl	containing,probes
R1764	T2708	T2709	det	the,sequence
R1765	T2709	T2707	dobj	sequence,containing
R1766	T2710	T2709	compound	Mcoln1,sequence
R1767	T2711	T2709	compound	coding,sequence
R1768	T2712	T2696	punct	", ",identify
R1769	T2713	T2714	det	the,transcripts
R177	T313	T312	pobj	MCOLN1,to
R1770	T2714	T2696	dobj	transcripts,identify
R1771	T2715	T2714	amod	same,transcripts
R1772	T2716	T2673	punct	.,is
R1777	T3016	T3017	nsubj	Comparison,shows
R1778	T3018	T3016	prep	of,Comparison
R1779	T3019	T3020	compound	Mcoln1,isoform
R178	T314	T309	punct	", ",is
R1780	T3020	T3018	pobj	isoform,of
R1781	T3021	T3020	nummod	1,isoform
R1782	T3022	T3016	prep	to,Comparison
R1783	T3023	T3024	poss	its,homologue
R1784	T3024	T3022	pobj	homologue,to
R1785	T3025	T3024	amod	human,homologue
R1786	T3026	T3027	amod	striking,similarity
R1787	T3027	T3017	dobj	similarity,shows
R1788	T3028	T3017	prep	at,shows
R1789	T3029	T3030	preconj	both,level
R179	T315	T316	advmod	especially,in
R1790	T3030	T3028	pobj	level,at
R1791	T3031	T3030	det	the,level
R1792	T3032	T3033	nmod	amino,acid
R1793	T3033	T3030	nmod	acid,level
R1794	T3034	T3033	cc	and,acid
R1795	T3035	T3033	conj	nucleotide,acid
R1796	T3036	T3017	punct	.,shows
R1797	T3038	T3039	det	All,six
R1798	T3039	T3040	nsubjpass	six,conserved
R1799	T3041	T3039	prep	of,six
R18	T131	T130	prep	in,seen
R180	T316	T309	prep	in,is
R1800	T3042	T3043	det	the,domains
R1801	T3043	T3041	pobj	domains,of
R1802	T3044	T3043	compound	transmembrane,domains
R1803	T3045	T3039	punct	", ",six
R1804	T3046	T3047	advmod	as,as
R1805	T3047	T3039	cc	as,six
R1806	T3048	T3047	advmod	well,as
R1807	T3049	T3050	det	the,channel
R1808	T3050	T3039	conj	channel,six
R1809	T3051	T3050	amod	putative,channel
R181	T317	T318	det	the,domains
R1810	T3052	T3050	compound	cation,channel
R1811	T3053	T3040	auxpass	are,conserved
R1812	T3054	T3040	advmod	highly,conserved
R1813	T3055	T3040	punct	.,conserved
R1814	T3057	T3058	det	The,motif
R1815	T3058	T3070	nsubjpass	motif,conserved
R1816	T3059	T3058	amod	putative,motif
R1817	T3060	T3058	nmod	di-leucine,motif
R1818	T3061	T3062	punct	(,X
R1819	T3062	T3060	parataxis	X,di-leucine
R182	T318	T316	pobj	domains,in
R1820	T3063	T3062	compound	L,X
R1821	T3064	T3062	punct	-,X
R1822	T3065	T3062	compound	L,X
R1823	T3066	T3062	punct	-,X
R1824	T3067	T3062	compound	X,X
R1825	T3068	T3062	punct	-,X
R1826	T3069	T3062	punct	),X
R1827	T3071	T3058	prep	at,motif
R1828	T3072	T3073	det	the,terminus
R1829	T3073	T3071	pobj	terminus,at
R183	T319	T318	compound	transmembrane,domains
R1830	T3074	T3073	compound	C,terminus
R1831	T3075	T3073	punct	-,terminus
R1832	T3076	T3058	punct	", ",motif
R1833	T3077	T3078	dep	which,act
R1834	T3078	T3058	relcl	act,motif
R1835	T3079	T3078	aux	may,act
R1836	T3080	T3078	prep	as,act
R1837	T3081	T3082	det	a,signal
R1838	T3082	T3080	pobj	signal,as
R1839	T3083	T3082	amod	late,signal
R184	T320	T318	cc	and,domains
R1840	T3084	T3085	amod	endosomal,lysosomal
R1841	T3085	T3082	amod	lysosomal,signal
R1842	T3086	T3085	punct	/,lysosomal
R1843	T3087	T3082	compound	targeting,signal
R1844	T3088	T3070	punct	", ",conserved
R1845	T3089	T3070	auxpass	is,conserved
R1846	T3090	T3070	advmod	also,conserved
R1847	T3091	T3092	punct	[,9
R1848	T3092	T3070	parataxis	9,conserved
R1849	T3093	T3092	punct	],9
R185	T321	T322	compound	ion,pore
R1850	T3094	T3070	punct	.,conserved
R1851	T3096	T3097	det	This,speculation
R1852	T3097	T3098	nsubjpass	speculation,supported
R1853	T3099	T3098	auxpass	is,supported
R1854	T3100	T3098	agent	by,supported
R1855	T3101	T3100	pobj	work,by
R1856	T3102	T3101	prep	with,work
R1857	T3103	T3104	compound	cup,5
R1858	T3104	T3102	pobj	5,with
R1859	T3105	T3104	punct	-,5
R186	T322	T323	compound	pore,region
R1860	T3106	T3107	punct	[,13
R1861	T3107	T3101	parataxis	13,work
R1862	T3108	T3107	punct	],13
R1863	T3109	T3101	punct	", ",work
R1864	T3110	T3111	det	the,homologue
R1865	T3111	T3101	appos	homologue,work
R1866	T3112	T3113	compound	c.,elegans
R1867	T3113	T3111	compound	elegans,homologue
R1868	T3114	T3111	prep	of,homologue
R1869	T3115	T3114	pobj	MCOLN1,of
R187	T323	T318	conj	region,domains
R1870	T3116	T3098	punct	", ",supported
R1871	T3117	T3118	mark	since,suggest
R1872	T3118	T3098	advcl	suggest,supported
R1873	T3119	T3120	amod	cellular,localization
R1874	T3120	T3121	compound	localization,studies
R1875	T3121	T3118	nsubj	studies,suggest
R1876	T3122	T3123	mark	that,found
R1877	T3123	T3118	ccomp	found,suggest
R1878	T3124	T3125	det	the,protein
R1879	T3125	T3123	nsubjpass	protein,found
R188	T324	T309	punct	.,is
R1880	T3126	T3123	auxpass	is,found
R1881	T3127	T3123	prep	in,found
R1882	T3128	T3129	det	the,endosomes
R1883	T3129	T3127	pobj	endosomes,in
R1884	T3130	T3129	amod	late,endosomes
R1885	T3131	T3129	cc	and,endosomes
R1886	T3132	T3131	punct	/,and
R1887	T3133	T3131	cc	or,and
R1888	T3134	T3129	conj	lysosomes,endosomes
R1889	T3135	T3098	punct	.,supported
R189	T326	T327	advmod	Also,conserved
R1890	T3137	T3138	det	The,gene
R1891	T3138	T3141	nsubj	gene,has
R1892	T3139	T3138	compound	mouse,gene
R1893	T3140	T3138	compound	Mcoln1,gene
R1894	T3142	T3143	nummod	two,isoforms
R1895	T3143	T3141	dobj	isoforms,has
R1896	T3144	T3145	advmod	alternatively,spliced
R1897	T3145	T3143	amod	spliced,isoforms
R1898	T3146	T3141	punct	", ",has
R1899	T3147	T3141	prep	with,has
R19	T132	T131	pobj	humans,in
R190	T328	T327	punct	", ",conserved
R1900	T3148	T3149	nsubj	isoform,having
R1901	T3149	T3147	pcomp	having,with
R1902	T3150	T3148	nummod	2,isoform
R1903	T3151	T3152	det	a,tail
R1904	T3152	T3149	dobj	tail,having
R1905	T3153	T3152	amod	different,tail
R1906	T3154	T3155	npadvmod	c,terminal
R1907	T3155	T3152	amod	terminal,tail
R1908	T3156	T3155	punct	-,terminal
R1909	T3157	T3152	amod	cytoplasmic,tail
R191	T329	T330	det	the,signal
R1910	T3158	T3141	punct	.,has
R1911	T3160	T3161	det	The,tail
R1912	T3161	T3169	nsubj	tail,lacks
R1913	T3162	T3161	amod	unique,tail
R1914	T3163	T3164	nummod	86,acid
R1915	T3164	T3161	nmod	acid,tail
R1916	T3165	T3164	nmod	amino,acid
R1917	T3166	T3167	npadvmod	c,terminal
R1918	T3167	T3161	amod	terminal,tail
R1919	T3168	T3167	punct	-,terminal
R192	T330	T327	nsubjpass	signal,conserved
R1920	T3170	T3171	det	the,signal
R1921	T3171	T3169	dobj	signal,lacks
R1922	T3172	T3171	amod	lysosomal,signal
R1923	T3173	T3171	compound	targeting,signal
R1924	T3174	T3169	cc	and,lacks
R1925	T3175	T3176	aux	does,contain
R1926	T3176	T3169	conj	contain,lacks
R1927	T3177	T3176	neg	not,contain
R1928	T3178	T3179	det	any,domains
R1929	T3179	T3176	dobj	domains,contain
R193	T331	T330	amod	late,signal
R1930	T3180	T3179	amod	conserved,domains
R1931	T3181	T3182	advmod	when,compared
R1932	T3182	T3176	advcl	compared,contain
R1933	T3183	T3182	prep	against,compared
R1934	T3184	T3185	det	the,databases
R1935	T3185	T3183	pobj	databases,against
R1936	T3186	T3185	amod	current,databases
R1937	T3187	T3185	compound	profile,databases
R1938	T3188	T3169	punct	.,lacks
R1939	T3190	T3191	nsubj	We,speculate
R194	T332	T333	amod	endosomal,lysosomal
R1940	T3192	T3193	mark	that,have
R1941	T3193	T3191	ccomp	have,speculate
R1942	T3194	T3195	det	this,protein
R1943	T3195	T3193	nsubj	protein,have
R1944	T3196	T3193	aux	may,have
R1945	T3197	T3198	amod	similar,function
R1946	T3198	T3193	dobj	function,have
R1947	T3199	T3198	compound	channel,function
R1948	T3200	T3198	cc	but,function
R1949	T3201	T3202	det	an,localization
R195	T333	T330	amod	lysosomal,signal
R1950	T3202	T3198	conj	localization,function
R1951	T3203	T3202	amod	alternate,localization
R1952	T3204	T3202	amod	subcellular,localization
R1953	T3205	T3191	punct	", ",speculate
R1954	T3206	T3191	cc	but,speculate
R1955	T3207	T3208	nsubjpass	this,proven
R1956	T3208	T3191	conj	proven,speculate
R1957	T3209	T3208	aux	must,proven
R1958	T3210	T3208	auxpass	be,proven
R1959	T3211	T3212	mark	once,raised
R196	T334	T333	punct	/,lysosomal
R1960	T3212	T3208	advcl	raised,proven
R1961	T3213	T3214	npadvmod	isoform,specific
R1962	T3214	T3216	amod	specific,antibodies
R1963	T3215	T3214	punct	-,specific
R1964	T3216	T3212	nsubjpass	antibodies,raised
R1965	T3217	T3212	auxpass	are,raised
R1966	T3218	T3208	punct	.,proven
R1967	T3220	T3221	advmod	However,suggest
R1968	T3222	T3221	punct	", ",suggest
R1969	T3223	T3224	poss	our,results
R197	T335	T330	compound	targeting,signal
R1970	T3224	T3221	nsubj	results,suggest
R1971	T3225	T3226	mark	that,be
R1972	T3226	T3221	advcl	be,suggest
R1973	T3227	T3228	amod	phenotypic,assessment
R1974	T3228	T3226	nsubj	assessment,be
R1975	T3229	T3228	prep	of,assessment
R1976	T3230	T3231	compound	Mcoln1,mice
R1977	T3231	T3229	pobj	mice,of
R1978	T3232	T3233	compound	knock,out
R1979	T3233	T3231	compound	out,mice
R198	T336	T327	auxpass	is,conserved
R1980	T3234	T3233	punct	-,out
R1981	T3235	T3226	aux	may,be
R1982	T3236	T3226	acomp	complicated,be
R1983	T3237	T3226	cc	and,be
R1984	T3238	T3239	mark	that,taken
R1985	T3239	T3226	conj	taken,be
R1986	T3240	T3239	nsubjpass	care,taken
R1987	T3241	T3239	aux	must,taken
R1988	T3242	T3239	auxpass	be,taken
R1989	T3243	T3244	advmod	when,interpreting
R199	T337	T327	punct	", ",conserved
R1990	T3244	T3239	advcl	interpreting,taken
R1991	T3245	T3244	dobj	data,interpreting
R1992	T3246	T3245	prep	on,data
R1993	T3247	T3248	compound	mouse,expression
R1994	T3248	T3246	pobj	expression,on
R1995	T3249	T3248	compound	gene,expression
R1996	T3250	T3248	cc	and,expression
R1997	T3251	T3248	conj	phenotype,expression
R1998	T3252	T3221	punct	.,suggest
R1999	T3254	T3255	advmod	Interestingly,seen
R20	T137	T138	compound	Mucolipidosis,type
R200	T338	T327	advcl	supporting,conserved
R2000	T3256	T3255	punct	", ",seen
R2001	T3257	T3258	det	the,isoform
R2002	T3258	T3255	nsubjpass	isoform,seen
R2003	T3259	T3258	amod	second,isoform
R2004	T3260	T3258	compound	Mcoln1,isoform
R2005	T3261	T3255	auxpass	is,seen
R2006	T3262	T3255	neg	not,seen
R2007	T3263	T3255	prep	in,seen
R2008	T3264	T3263	pobj	humans,in
R2009	T3265	T3255	cc	and,seen
R201	T339	T340	det	the,hypothesis
R2010	T3266	T3267	det	the,sequence
R2011	T3267	T3268	nsubjpass	sequence,conserved
R2012	T3268	T3255	conj	conserved,seen
R2013	T3269	T3267	prep	of,sequence
R2014	T3270	T3271	det	the,region
R2015	T3271	T3269	pobj	region,of
R2016	T3272	T3273	advmod	alternatively,spliced
R2017	T3273	T3271	amod	spliced,region
R2018	T3274	T3268	auxpass	is,conserved
R2019	T3275	T3268	neg	not,conserved
R202	T340	T338	dobj	hypothesis,supporting
R2020	T3276	T3268	prep	between,conserved
R2021	T3277	T3276	pobj	man,between
R2022	T3278	T3277	cc	and,man
R2023	T3279	T3277	conj	mouse,man
R2024	T3280	T3255	punct	.,seen
R2025	T3282	T3283	prep	To,reported
R2026	T3284	T3282	pobj	date,To
R2027	T3285	T3283	punct	", ",reported
R2028	T3286	T3287	advmod	very,few
R2029	T3287	T3288	amod	few,genes
R203	T341	T342	mark	that,localized
R2030	T3288	T3283	nsubjpass	genes,reported
R2031	T3289	T3283	aux	have,reported
R2032	T3290	T3283	auxpass	been,reported
R2033	T3291	T3292	dep	that,show
R2034	T3292	T3283	ccomp	show,reported
R2035	T3293	T3294	npadvmod	species,specific
R2036	T3294	T3295	amod	specific,variants
R2037	T3295	T3292	dobj	variants,show
R2038	T3296	T3295	amod	alternative,variants
R2039	T3297	T3295	compound	splice,variants
R204	T342	T340	acl	localized,hypothesis
R2040	T3298	T3283	punct	.,reported
R2041	T3300	T3301	nsubj	MOG,has
R2042	T3302	T3300	punct	", ",MOG
R2043	T3303	T3304	compound	myelin,oligodendrocyte
R2044	T3304	T3306	compound	oligodendrocyte,glycoprotein
R2045	T3305	T3304	punct	/,oligodendrocyte
R2046	T3306	T3300	appos	glycoprotein,MOG
R2047	T3307	T3301	punct	", ",has
R2048	T3308	T3309	amod	many,variants
R2049	T3309	T3301	dobj	variants,has
R205	T343	T344	det	the,protein
R2050	T3310	T3309	amod	different,variants
R2051	T3311	T3309	compound	splice,variants
R2052	T3312	T3301	prep	in,has
R2053	T3313	T3312	pobj	humans,in
R2054	T3314	T3315	dep	that,found
R2055	T3315	T3301	ccomp	found,has
R2056	T3316	T3315	auxpass	are,found
R2057	T3317	T3315	neg	not,found
R2058	T3318	T3315	prep	in,found
R2059	T3319	T3318	pobj	mice,in
R206	T344	T342	nsubjpass	protein,localized
R2060	T3320	T3321	punct	[,17
R2061	T3321	T3301	parataxis	17,has
R2062	T3322	T3321	punct	],17
R2063	T3323	T3301	punct	.,has
R2064	T3325	T3326	nsubj	ATP11B,has
R2065	T3327	T3325	punct	", ",ATP11B
R2066	T3328	T3329	det	a,ATPase
R2067	T3329	T3325	appos	ATPase,ATP11B
R2068	T3330	T3331	compound	P,type
R2069	T3331	T3329	compound	type,ATPase
R207	T345	T342	auxpass	is,localized
R2070	T3332	T3331	punct	-,type
R2071	T3333	T3326	punct	", ",has
R2072	T3334	T3335	det	a,variant
R2073	T3335	T3326	dobj	variant,has
R2074	T3336	T3337	npadvmod	rabbit,specific
R2075	T3337	T3335	amod	specific,variant
R2076	T3338	T3337	punct	-,specific
R2077	T3339	T3335	compound	splice,variant
R2078	T3340	T3341	dep	that,deletes
R2079	T3341	T3335	relcl	deletes,variant
R208	T346	T342	prep	to,localized
R2080	T3342	T3343	det	a,domain
R2081	T3343	T3341	dobj	domain,deletes
R2082	T3344	T3343	amod	transmembrane,domain
R2083	T3345	T3341	cc	and,deletes
R2084	T3346	T3347	advmod	therefore,alters
R2085	T3347	T3341	conj	alters,deletes
R2086	T3348	T3347	advmod	likely,alters
R2087	T3349	T3350	det	the,function
R2088	T3350	T3347	dobj	function,alters
R2089	T3351	T3350	amod	putative,function
R209	T347	T348	det	these,membranes
R2090	T3352	T3350	prep	of,function
R2091	T3353	T3354	det	the,protein
R2092	T3354	T3352	pobj	protein,of
R2093	T3355	T3356	punct	[,18
R2094	T3356	T3326	parataxis	18,has
R2095	T3357	T3356	punct	],18
R2096	T3358	T3326	punct	.,has
R2097	T3360	T3361	nsubj	Sequencing,led
R2098	T3362	T3360	prep	of,Sequencing
R2099	T3363	T3364	det	the,genome
R21	T138	T139	nsubj	type,is
R210	T348	T346	pobj	membranes,to
R2100	T3364	T3362	pobj	genome,of
R2101	T3365	T3364	amod	human,genome
R2102	T3366	T3361	aux	has,led
R2103	T3367	T3361	prep	to,led
R2104	T3368	T3367	pobj	estimates,to
R2105	T3369	T3368	prep	of,estimates
R2106	T3370	T3371	advmod	approximately,"32,000"
R2107	T3371	T3372	nummod	"32,000",genes
R2108	T3372	T3369	pobj	genes,of
R2109	T3373	T3361	punct	", ",led
R211	T349	T348	compound	vesicle,membranes
R2110	T3374	T3375	det	a,surprise
R2111	T3375	T3361	npadvmod	surprise,led
R2112	T3376	T3375	amod	total,surprise
R2113	T3377	T3375	prep	given,surprise
R2114	T3378	T3379	det	the,estimates
R2115	T3379	T3377	pobj	estimates,given
R2116	T3380	T3379	amod	previous,estimates
R2117	T3381	T3382	advmod	significantly,higher
R2118	T3382	T3379	amod	higher,estimates
R2119	T3383	T3384	dep	that,based
R212	T350	T327	punct	.,conserved
R2120	T3384	T3379	relcl	based,estimates
R2121	T3385	T3384	auxpass	were,based
R2122	T3386	T3384	prep	on,based
R2123	T3387	T3388	det	the,number
R2124	T3388	T3386	pobj	number,on
R2125	T3389	T3388	prep	of,number
R2126	T3390	T3391	amod	expressed,tags
R2127	T3391	T3389	pobj	tags,of
R2128	T3392	T3391	compound	sequence,tags
R2129	T3393	T3391	punct	(,tags
R213	T352	T353	prep	To,are
R2130	T3394	T3391	appos	ESTs,tags
R2131	T3395	T3388	punct	),number
R2132	T3396	T3388	prep	in,number
R2133	T3397	T3398	det	the,databases
R2134	T3398	T3396	pobj	databases,in
R2135	T3399	T3398	amod	public,databases
R2136	T3400	T3361	punct	.,led
R2137	T3402	T3403	det	This,disparity
R2138	T3403	T3405	nsubj	disparity,suggests
R2139	T3404	T3403	amod	apparent,disparity
R214	T354	T352	pobj	date,To
R2140	T3406	T3407	det	a,role
R2141	T3407	T3405	dobj	role,suggests
R2142	T3408	T3407	amod	major,role
R2143	T3409	T3405	prep	for,suggests
R2144	T3410	T3411	amod	alternative,splicing
R2145	T3411	T3409	pobj	splicing,for
R2146	T3412	T3405	prep	in,suggests
R2147	T3413	T3412	pcomp	creating,in
R2148	T3414	T3415	amod	genetic,complexity
R2149	T3415	T3413	dobj	complexity,creating
R215	T355	T353	punct	", ",are
R2150	T3416	T3405	punct	", ",suggests
R2151	T3417	T3405	cc	and,suggests
R2152	T3418	T3419	aux	has,brought
R2153	T3419	T3405	conj	brought,suggests
R2154	T3420	T3421	det	the,study
R2155	T3421	T3419	dobj	study,brought
R2156	T3422	T3421	prep	of,study
R2157	T3423	T3424	compound	splicing,regulation
R2158	T3424	T3422	pobj	regulation,of
R2159	T3425	T3419	prep	to,brought
R216	T356	T353	expl	there,are
R2160	T3426	T3427	det	the,forefront
R2161	T3427	T3425	pobj	forefront,to
R2162	T3428	T3427	prep	of,forefront
R2163	T3429	T3430	amod	molecular,genetics
R2164	T3430	T3428	pobj	genetics,of
R2165	T3431	T3405	punct	.,suggests
R2166	T3433	T3434	nsubj	It,is
R2167	T3435	T3434	acomp	likely,is
R2168	T3436	T3437	mark	that,identified
R2169	T3437	T3434	ccomp	identified,is
R217	T357	T358	advmod	very,few
R2170	T3438	T3439	det	an,abundance
R2171	T3439	T3437	nsubjpass	abundance,identified
R2172	T3440	T3439	prep	of,abundance
R2173	T3441	T3442	npadvmod	species,specific
R2174	T3442	T3444	amod	specific,variants
R2175	T3443	T3442	punct	-,specific
R2176	T3444	T3440	pobj	variants,of
R2177	T3445	T3444	compound	splice,variants
R2178	T3446	T3437	aux	will,identified
R2179	T3447	T3437	auxpass	be,identified
R218	T358	T359	amod	few,reports
R2180	T3448	T3449	mark	as,progresses
R2181	T3449	T3437	advcl	progresses,identified
R2182	T3450	T3451	det	the,characterization
R2183	T3451	T3449	nsubj	characterization,progresses
R2184	T3452	T3451	prep	of,characterization
R2185	T3453	T3454	advmod	alternatively,spliced
R2186	T3454	T3455	amod	spliced,transcripts
R2187	T3455	T3452	pobj	transcripts,of
R2188	T3456	T3434	punct	.,is
R219	T359	T353	attr	reports,are
R2190	T3569	T3570	nsubj	We,conducted
R2191	T3571	T3572	compound	database,searches
R2192	T3572	T3570	dobj	searches,conducted
R2193	T3573	T3570	advcl	using,conducted
R2194	T3574	T3573	dobj	BLAST,using
R2195	T3575	T3574	cc	and,BLAST
R2196	T3576	T3577	compound	Draft,Browser
R2197	T3577	T3574	conj	Browser,BLAST
R2198	T3578	T3579	compound	Human,Genome
R2199	T3579	T3577	compound	Genome,Browser
R22	T140	T138	nummod	IV,type
R220	T360	T359	acl	describing,reports
R2200	T3580	T3570	punct	.,conducted
R2201	T3582	T3583	nsubjpass	Sequences,used
R2202	T3584	T3582	prep	from,Sequences
R2203	T3585	T3584	pobj	UniGene,from
R2204	T3586	T3583	auxpass	were,used
R2205	T3587	T3588	aux	to,confirm
R2206	T3588	T3583	advcl	confirm,used
R2207	T3589	T3590	det	the,sequence
R2208	T3590	T3588	dobj	sequence,confirm
R2209	T3591	T3590	compound	Mcoln1,sequence
R221	T361	T362	npadvmod	species,specific
R2210	T3592	T3583	punct	.,used
R2211	T3594	T3595	nsubj	We,performed
R2212	T3596	T3597	compound	motif,searches
R2213	T3597	T3595	dobj	searches,performed
R2214	T3598	T3595	advcl	using,performed
R2215	T3599	T3598	dobj	ProfileScan,using
R2216	T3600	T3599	cc	and,ProfileScan
R2217	T3601	T3599	conj	TMPred,ProfileScan
R2218	T3602	T3595	cc	and,performed
R2219	T3603	T3595	conj	alignment,performed
R222	T362	T364	amod	specific,variants
R2220	T3604	T3603	prep	of,alignment
R2221	T3605	T3606	compound	protein,sequences
R2222	T3606	T3604	pobj	sequences,of
R2223	T3607	T3603	advcl	using,alignment
R2224	T3608	T3607	dobj	Pileup,using
R2225	T3609	T3610	punct	(,GCG
R2226	T3610	T3608	parataxis	GCG,Pileup
R2227	T3611	T3610	punct	),GCG
R2228	T3612	T3608	cc	and,Pileup
R2229	T3613	T3608	conj	Boxshade,Pileup
R223	T363	T362	punct	-,specific
R2230	T3614	T3595	punct	.,performed
R2231	T3623	T3624	compound	DNA,Sequencing
R2232	T3626	T3627	compound	I.M.A.G.E.,Clones
R2233	T3627	T3628	nsubjpass	Clones,purchased
R2234	T3629	T3628	auxpass	were,purchased
R2235	T3630	T3628	prep	from,purchased
R2236	T3631	T3632	compound	Research,Genetics
R2237	T3632	T3630	pobj	Genetics,from
R2238	T3633	T3634	punct	(,Huntsville
R2239	T3634	T3632	parataxis	Huntsville,Genetics
R224	T364	T360	dobj	variants,describing
R2240	T3635	T3634	punct	", ",Huntsville
R2241	T3636	T3634	npadvmod	AL,Huntsville
R2242	T3637	T3634	punct	),Huntsville
R2243	T3638	T3628	punct	.,purchased
R2244	T3640	T3641	nsubjpass	Sequencing,performed
R2245	T3642	T3641	auxpass	was,performed
R2246	T3643	T3641	advcl	using,performed
R2247	T3644	T3645	det	the,kit
R2248	T3645	T3643	dobj	kit,using
R2249	T3646	T3645	compound	AmpliCycle,kit
R225	T365	T364	compound	splice,variants
R2250	T3647	T3645	compound	sequencing,kit
R2251	T3648	T3645	prep	from,kit
R2252	T3649	T3650	compound	Applied,Biosystems
R2253	T3650	T3648	pobj	Biosystems,from
R2254	T3651	T3652	punct	(,City
R2255	T3652	T3650	parataxis	City,Biosystems
R2256	T3653	T3652	compound	Foster,City
R2257	T3654	T3652	punct	", ",City
R2258	T3655	T3652	npadvmod	CA,City
R2259	T3656	T3652	punct	),City
R226	T366	T353	punct	.,are
R2260	T3657	T3643	prep	on,using
R2261	T3658	T3659	det	a,sequencer
R2262	T3659	T3657	pobj	sequencer,on
R2263	T3660	T3661	nmod	Genomyx,LR
R2264	T3661	T3659	nmod	LR,sequencer
R2265	T3662	T3659	amod	programmable,sequencer
R2266	T3663	T3659	compound	DNA,sequencer
R2267	T3664	T3641	punct	.,performed
R2268	T3689	T3690	compound	RT,PCR
R2269	T3690	T3692	compound	PCR,Reaction
R227	T368	T369	mark	While,is
R2270	T3691	T3690	punct	-,PCR
R2271	T3694	T3695	nmod	BALB,c
R2272	T3695	T3697	nmod	c,mouse
R2273	T3696	T3695	punct	/,c
R2274	T3697	T3698	nmod	mouse,brain
R2275	T3698	T3699	nmod	brain,RNA
R2276	T3699	T3701	nsubjpass	RNA,purchased
R2277	T3700	T3699	amod	total,RNA
R2278	T3702	T3701	auxpass	was,purchased
R2279	T3703	T3701	prep	from,purchased
R228	T369	T373	advcl	is,suggests
R2280	T3704	T3705	compound	Clontech,Laboratories
R2281	T3705	T3703	pobj	Laboratories,from
R2282	T3706	T3707	punct	(,Alto
R2283	T3707	T3705	parataxis	Alto,Laboratories
R2284	T3708	T3707	compound	Palo,Alto
R2285	T3709	T3707	punct	", ",Alto
R2286	T3710	T3707	npadvmod	CA,Alto
R2287	T3711	T3707	punct	),Alto
R2288	T3712	T3701	cc	and,purchased
R2289	T3713	T3701	conj	made,purchased
R229	T370	T369	nsubj	identification,is
R2290	T3714	T3713	prep	to,made
R2291	T3715	T3716	det	a,concentration
R2292	T3716	T3714	pobj	concentration,to
R2293	T3717	T3718	nummod	1,μg
R2294	T3718	T3716	nmod	μg,concentration
R2295	T3719	T3720	punct	/,ml
R2296	T3720	T3718	prep	ml,μg
R2297	T3721	T3701	punct	.,purchased
R2298	T3723	T3724	det	This,RNA
R2299	T3724	T3725	nsubjpass	RNA,used
R23	T141	T138	punct	(,type
R230	T371	T370	prep	of,identification
R2300	T3726	T3725	auxpass	was,used
R2301	T3727	T3725	prep	as,used
R2302	T3728	T3729	det	a,template
R2303	T3729	T3727	pobj	template,as
R2304	T3730	T3731	aux	to,create
R2305	T3731	T3725	advcl	create,used
R2306	T3732	T3731	dobj	cDNA,create
R2307	T3733	T3731	prep	via,create
R2308	T3734	T3735	compound	RT,PCR
R2309	T3735	T3733	pobj	PCR,via
R231	T372	T371	pobj	Mcoln1,of
R2310	T3736	T3735	punct	-,PCR
R2311	T3737	T3731	advcl	using,create
R2312	T3738	T3739	amod	random,hexamers
R2313	T3739	T3737	dobj	hexamers,using
R2314	T3740	T3739	cc	and,hexamers
R2315	T3741	T3742	compound	oligo,primer
R2316	T3742	T3739	conj	primer,hexamers
R2317	T3743	T3742	compound	dT,primer
R2318	T3744	T3725	punct	.,used
R2319	T3746	T3747	det	The,product
R232	T374	T369	acomp	crucial,is
R2320	T3747	T3749	nsubjpass	product,used
R2321	T3748	T3747	compound	RT,product
R2322	T3750	T3749	auxpass	was,used
R2323	T3751	T3752	aux	to,confirm
R2324	T3752	T3749	advcl	confirm,used
R2325	T3753	T3754	det	the,form
R2326	T3754	T3752	dobj	form,confirm
R2327	T3755	T3756	advmod	alternatively,spliced
R2328	T3756	T3754	amod	spliced,form
R2329	T3757	T3752	advcl	using,confirm
R233	T375	T374	prep	to,crucial
R2330	T3758	T3757	dobj	primers,using
R2331	T3759	T3758	punct	: ,primers
R2332	T3760	T3761	nummod	5,3
R2333	T3761	T3758	appos	3,primers
R2334	T3762	T3760	punct	',5
R2335	T3763	T3761	punct	-,3
R2336	T3764	T3761	compound	CATCTACCTGGGCTATTGC,3
R2337	T3765	T3761	punct	-,3
R2338	T3766	T3761	punct	',3
R2339	T3767	T3768	punct	(,forward
R234	T376	T377	det	the,development
R2340	T3768	T3761	parataxis	forward,3
R2341	T3769	T3768	punct	),forward
R2342	T3770	T3761	cc	and,3
R2343	T3771	T3772	nummod	5,3
R2344	T3772	T3761	conj	3,3
R2345	T3773	T3771	punct	',5
R2346	T3774	T3772	punct	-,3
R2347	T3775	T3772	compound	GCTCTCAGGTGGTGGACAC,3
R2348	T3776	T3772	punct	-,3
R2349	T3777	T3772	punct	',3
R235	T377	T375	pobj	development,to
R2350	T3778	T3779	punct	(,reverse
R2351	T3779	T3772	parataxis	reverse,3
R2352	T3780	T3779	punct	),reverse
R2353	T3781	T3757	prep	in,using
R2354	T3782	T3783	det	a,reaction
R2355	T3783	T3781	pobj	reaction,in
R2356	T3784	T3783	compound	PCR,reaction
R2357	T3785	T3757	prep	with,using
R2358	T3786	T3787	det	an,temperature
R2359	T3787	T3785	pobj	temperature,with
R236	T378	T377	prep	of,development
R2360	T3788	T3787	amod	annealing,temperature
R2361	T3789	T3787	prep	of,temperature
R2362	T3790	T3791	nummod	61,°C
R2363	T3791	T3789	pobj	°C,of
R2364	T3792	T3749	punct	.,used
R2366	T3835	T3836	compound	Southern,Blot
R2367	T3836	T3837	compound	Blot,Analysis
R2368	T3839	T3840	amod	Total,DNA
R2369	T3840	T3843	nsubjpass	DNA,digested
R237	T379	T380	compound	mouse,models
R2370	T3841	T3840	nmod	mouse,DNA
R2371	T3842	T3840	amod	genomic,DNA
R2372	T3844	T3843	auxpass	was,digested
R2373	T3845	T3843	advcl	using,digested
R2374	T3846	T3845	dobj	EcoRI,using
R2375	T3847	T3846	punct	", ",EcoRI
R2376	T3848	T3846	conj	BamHI,EcoRI
R2377	T3849	T3848	punct	", ",BamHI
R2378	T3850	T3848	conj	PstI,BamHI
R2379	T3851	T3850	punct	", ",PstI
R238	T380	T378	pobj	models,of
R2380	T3852	T3850	cc	and,PstI
R2381	T3853	T3850	conj	XbaI,PstI
R2382	T3854	T3843	punct	.,digested
R2383	T3856	T3857	det	The,digests
R2384	T3857	T3858	nsubjpass	digests,electrophoresed
R2385	T3859	T3858	auxpass	were,electrophoresed
R2386	T3860	T3858	prep	on,electrophoresed
R2387	T3861	T3862	det	a,gel
R2388	T3862	T3860	pobj	gel,on
R2389	T3863	T3864	nummod	1,%
R239	T381	T377	prep	for,development
R2390	T3864	T3862	compound	%,gel
R2391	T3865	T3862	compound	agarose,gel
R2392	T3866	T3858	prep	at,electrophoresed
R2393	T3867	T3866	pobj	60V,at
R2394	T3868	T3858	advmod	overnight,electrophoresed
R2395	T3869	T3858	cc	and,electrophoresed
R2396	T3870	T3871	auxpass	were,transferred
R2397	T3871	T3858	conj	transferred,electrophoresed
R2398	T3872	T3871	prep	onto,transferred
R2399	T3873	T3874	det	a,membrane
R24	T142	T138	appos	MLIV,type
R240	T382	T381	pobj	MLIV,for
R2400	T3874	T3872	pobj	membrane,onto
R2401	T3875	T3874	nmod	Hybond,membrane
R2402	T3876	T3875	amod	N+,Hybond
R2403	T3877	T3871	prep	from,transferred
R2404	T3878	T3879	compound	Amersham,Biotech
R2405	T3879	T3877	pobj	Biotech,from
R2406	T3880	T3879	compound	Pharmacia,Biotech
R2407	T3881	T3882	punct	(,Piscataway
R2408	T3882	T3879	parataxis	Piscataway,Biotech
R2409	T3883	T3882	punct	", ",Piscataway
R241	T383	T373	punct	", ",suggests
R2410	T3884	T3882	npadvmod	NJ,Piscataway
R2411	T3885	T3882	punct	),Piscataway
R2412	T3886	T3858	punct	.,electrophoresed
R2413	T3888	T3889	det	A,fragment
R2414	T3889	T3895	nsubjpass	fragment,used
R2415	T3890	T3891	compound	32P,dATP
R2416	T3891	T3893	npadvmod	dATP,labeled
R2417	T3892	T3891	punct	-,dATP
R2418	T3893	T3889	amod	labeled,fragment
R2419	T3894	T3889	compound	PCR,fragment
R242	T384	T385	det	the,fact
R2420	T3896	T3889	prep	of,fragment
R2421	T3897	T3898	det	the,region
R2422	T3898	T3896	pobj	region,of
R2423	T3899	T3898	compound	Mcoln1,region
R2424	T3900	T3898	compound	coding,region
R2425	T3901	T3898	acl	corresponding,region
R2426	T3902	T3901	prep	to,corresponding
R2427	T3903	T3902	pobj	exon,to
R2428	T3904	T3903	nummod	2,exon
R2429	T3905	T3895	auxpass	was,used
R243	T385	T373	nsubj	fact,suggests
R2430	T3906	T3895	prep	as,used
R2431	T3907	T3908	det	a,probe
R2432	T3908	T3906	pobj	probe,as
R2433	T3909	T3910	punct	(,3
R2434	T3910	T3895	parataxis	3,used
R2435	T3911	T3910	nmod	primers,3
R2436	T3912	T3910	nummod	5,3
R2437	T3913	T3912	punct	',5
R2438	T3914	T3910	punct	-,3
R2439	T3915	T3910	compound	CCCCACAGAAGAGGAAGAC,3
R244	T386	T387	mark	that,are
R2440	T3916	T3910	punct	-,3
R2441	T3917	T3910	punct	',3
R2442	T3918	T3919	punct	(,forward
R2443	T3919	T3910	parataxis	forward,3
R2444	T3920	T3919	punct	),forward
R2445	T3921	T3910	cc	and,3
R2446	T3922	T3923	nummod	5,3
R2447	T3923	T3910	conj	3,3
R2448	T3924	T3922	punct	',5
R2449	T3925	T3923	punct	-,3
R245	T387	T385	acl	are,fact
R2450	T3926	T3923	compound	AGATCTTGACCACCTGCAG,3
R2451	T3927	T3923	punct	-,3
R2452	T3928	T3923	punct	',3
R2453	T3929	T3930	punct	(,reverse
R2454	T3930	T3923	parataxis	reverse,3
R2455	T3931	T3930	punct	),reverse
R2456	T3932	T3910	prep	with,3
R2457	T3933	T3934	det	an,temperature
R2458	T3934	T3932	pobj	temperature,with
R2459	T3935	T3934	amod	annealing,temperature
R246	T388	T387	expl	there,are
R2460	T3936	T3934	prep	of,temperature
R2461	T3937	T3938	nummod	59,°C
R2462	T3938	T3936	pobj	°C,of
R2463	T3939	T3910	punct	),3
R2464	T3940	T3895	punct	.,used
R2465	T3942	T3943	nsubjpass	Hybridization,carried
R2466	T3944	T3942	cc	and,Hybridization
R2467	T3945	T3942	conj	washes,Hybridization
R2468	T3946	T3943	auxpass	were,carried
R2469	T3947	T3943	prt	out,carried
R247	T389	T390	nummod	two,transcripts
R2470	T3948	T3943	prep	in,carried
R2471	T3949	T3950	amod	standard,conditions
R2472	T3950	T3948	pobj	conditions,in
R2473	T3951	T3952	punct	[,16
R2474	T3952	T3943	parataxis	16,carried
R2475	T3953	T3952	punct	],16
R2476	T3954	T3943	punct	.,carried
R2479	T4011	T4012	compound	Northern,Blot
R248	T390	T387	attr	transcripts,are
R2480	T4012	T4013	compound	Blot,Analysis
R2481	T4015	T4016	nmod	Mouse,tissue
R2482	T4016	T4020	nmod	tissue,blot
R2483	T4017	T4016	nmod	embryo,tissue
R2484	T4018	T4016	amod	multiple,tissue
R2485	T4019	T4016	punct	-,tissue
R2486	T4020	T4022	nmod	blot,filters
R2487	T4021	T4020	amod	northern,blot
R2488	T4022	T4031	nsubjpass	filters,purchased
R2489	T4023	T4020	cc	and,blot
R249	T391	T387	prep	in,are
R2490	T4024	T4025	nmod	mouse,tissue
R2491	T4025	T4029	nmod	tissue,blot
R2492	T4026	T4025	amod	adult,tissue
R2493	T4027	T4025	amod	multiple,tissue
R2494	T4028	T4025	punct	-,tissue
R2495	T4029	T4020	conj	blot,blot
R2496	T4030	T4029	amod	northern,blot
R2497	T4032	T4031	auxpass	were,purchased
R2498	T4033	T4031	prep	from,purchased
R2499	T4034	T4035	compound	Clontech,Laboratories
R25	T143	T139	punct	),is
R250	T392	T391	pobj	mice,in
R2500	T4035	T4033	pobj	Laboratories,from
R2501	T4036	T4037	punct	(,Alto
R2502	T4037	T4035	parataxis	Alto,Laboratories
R2503	T4038	T4037	compound	Palo,Alto
R2504	T4039	T4037	punct	", ",Alto
R2505	T4040	T4037	npadvmod	CA,Alto
R2506	T4041	T4037	punct	),Alto
R2507	T4042	T4031	punct	.,purchased
R2508	T4044	T4045	det	The,filters
R2509	T4045	T4046	nsubjpass	filters,hybridized
R251	T393	T394	det	an,function
R2510	T4047	T4046	auxpass	were,hybridized
R2511	T4048	T4046	prep	with,hybridized
R2512	T4049	T4050	det	the,fragment
R2513	T4050	T4048	pobj	fragment,with
R2514	T4051	T4052	compound	32P,dATP
R2515	T4052	T4054	npadvmod	dATP,labeled
R2516	T4053	T4052	punct	-,dATP
R2517	T4054	T4050	amod	labeled,fragment
R2518	T4055	T4050	compound	DNA,fragment
R2519	T4056	T4050	prep	of,fragment
R252	T394	T373	dobj	function,suggests
R2520	T4057	T4058	compound	Mcoln1,region
R2521	T4058	T4056	pobj	region,of
R2522	T4059	T4058	compound	coding,region
R2523	T4060	T4058	acl	corresponding,region
R2524	T4061	T4060	prep	to,corresponding
R2525	T4062	T4061	pobj	exon,to
R2526	T4063	T4062	nummod	2,exon
R2527	T4064	T4065	punct	(,see
R2528	T4065	T4046	parataxis	see,hybridized
R2529	T4066	T4065	advmod	above,see
R253	T395	T394	amod	additional,function
R2530	T4067	T4065	punct	),see
R2531	T4068	T4046	punct	.,hybridized
R2532	T4070	T4071	prep	For,generated
R2533	T4072	T4073	det	the,transcript
R2534	T4073	T4070	pobj	transcript,For
R2535	T4074	T4073	amod	alternative,transcript
R2536	T4075	T4071	punct	", ",generated
R2537	T4076	T4077	det	a,probe
R2538	T4077	T4071	nsubjpass	probe,generated
R2539	T4078	T4071	auxpass	was,generated
R254	T396	T395	cc	or,additional
R2540	T4079	T4071	prep	in,generated
R2541	T4080	T4081	det	the,region
R2542	T4081	T4079	pobj	region,in
R2543	T4082	T4081	prep	between,region
R2544	T4083	T4084	nmod	exons,12
R2545	T4084	T4082	pobj	12,between
R2546	T4085	T4084	cc	and,12
R2547	T4086	T4084	conj	13,12
R2548	T4087	T4071	prep	with,generated
R2549	T4088	T4089	nmod	primers,3
R255	T397	T395	conj	alternate,additional
R2550	T4089	T4087	pobj	3,with
R2551	T4090	T4089	nmod	5,3
R2552	T4091	T4090	punct	',5
R2553	T4092	T4089	punct	-,3
R2554	T4093	T4089	compound	GTGTCCACCACCTGAGAG,3
R2555	T4094	T4089	punct	-,3
R2556	T4095	T4089	punct	',3
R2557	T4096	T4097	punct	(,forward
R2558	T4097	T4089	parataxis	forward,3
R2559	T4098	T4097	punct	),forward
R256	T398	T394	prep	of,function
R2560	T4099	T4089	cc	and,3
R2561	T4100	T4101	nmod	5,3
R2562	T4101	T4089	conj	3,3
R2563	T4102	T4100	punct	',5
R2564	T4103	T4101	punct	-,3
R2565	T4104	T4101	compound	GAAGTAGCATTCCTGCAGGC,3
R2566	T4105	T4101	punct	-,3
R2567	T4106	T4101	punct	',3
R2568	T4107	T4108	punct	(,reverse
R2569	T4108	T4101	parataxis	reverse,3
R257	T399	T400	det	the,gene
R2570	T4109	T4108	punct	),reverse
R2571	T4110	T4071	prep	with,generated
R2572	T4111	T4112	det	an,temperature
R2573	T4112	T4110	pobj	temperature,with
R2574	T4113	T4112	amod	annealing,temperature
R2575	T4114	T4112	prep	of,temperature
R2576	T4115	T4116	nummod	62,°C
R2577	T4116	T4114	pobj	°C,of
R2578	T4117	T4071	punct	.,generated
R2579	T4119	T4120	det	The,filters
R258	T400	T398	pobj	gene,of
R2580	T4120	T4121	nsubjpass	filters,hybridized
R2581	T4122	T4121	auxpass	were,hybridized
R2582	T4123	T4121	advmod	then,hybridized
R2583	T4124	T4121	prep	with,hybridized
R2584	T4125	T4126	compound	β,actin
R2585	T4126	T4128	compound	actin,probes
R2586	T4127	T4126	punct	-,actin
R2587	T4128	T4124	pobj	probes,with
R2588	T4129	T4121	punct	.,hybridized
R2589	T4131	T4132	nsubjpass	Hybridizations,carried
R259	T401	T402	dep	that,complicate
R2590	T4133	T4131	cc	and,Hybridizations
R2591	T4134	T4131	conj	washes,Hybridizations
R2592	T4135	T4132	auxpass	were,carried
R2593	T4136	T4132	prt	out,carried
R2594	T4137	T4132	prep	in,carried
R2595	T4138	T4139	amod	standard,conditions
R2596	T4139	T4137	pobj	conditions,in
R2597	T4140	T4132	punct	", ",carried
R2598	T4141	T4132	prep	with,carried
R2599	T4142	T4143	det	the,stripping
R26	T144	T145	det	an,disorder
R260	T402	T394	relcl	complicate,function
R2600	T4143	T4141	pobj	stripping,with
R2601	T4144	T4143	prep	of,stripping
R2602	T4145	T4146	advmod	previously,bound
R2603	T4146	T4147	amod	bound,probes
R2604	T4147	T4144	pobj	probes,of
R2605	T4148	T4149	advmod	in,between
R2606	T4149	T4143	advmod	between,stripping
R2607	T4150	T4151	punct	[,16
R2608	T4151	T4132	parataxis	16,carried
R2609	T4152	T4151	punct	],16
R261	T403	T402	aux	may,complicate
R2610	T4153	T4132	punct	.,carried
R2613	T4216	T4217	amod	Physical,map
R2614	T4218	T4216	cc	and,Physical
R2615	T4219	T4216	conj	transcript,Physical
R2616	T4220	T4217	prep	of,map
R2617	T4221	T4222	det	the,region
R2618	T4222	T4220	pobj	region,of
R2619	T4223	T4224	compound	Mcoln1,gene
R262	T404	T405	amod	phenotypic,assessment
R2620	T4224	T4222	compound	gene,region
R2621	T4225	T4217	punct	.,map
R2622	T4227	T4228	punct	(,A
R2623	T4228	T4229	meta	A,map
R2624	T4230	T4228	punct	),A
R2625	T4231	T4229	amod	Physical,map
R2626	T4232	T4229	amod	showing,map
R2627	T4233	T4234	det	a,segment
R2628	T4234	T4229	appos	segment,map
R2629	T4235	T4236	nummod	590,kb
R263	T405	T402	dobj	assessment,complicate
R2630	T4236	T4234	compound	kb,segment
R2631	T4237	T4234	prep	of,segment
R2632	T4238	T4239	compound	mouse,chromosome
R2633	T4239	T4237	pobj	chromosome,of
R2634	T4240	T4239	nummod	8,chromosome
R2635	T4241	T4234	amod	syntenous,segment
R2636	T4242	T4241	prep	to,syntenous
R2637	T4243	T4244	amod	human,chromosome
R2638	T4244	T4242	pobj	chromosome,to
R2639	T4245	T4244	nummod	19,chromosome
R264	T406	T373	punct	.,suggests
R2640	T4246	T4234	punct	", ",segment
R2641	T4247	T4234	acl	anchored,segment
R2642	T4248	T4247	agent	by,anchored
R2643	T4249	T4250	det	the,Insr
R2644	T4250	T4248	pobj	Insr,by
R2645	T4251	T4250	compound	genes,Insr
R2646	T4252	T4250	cc	and,Insr
R2647	T4253	T4250	conj	Nte,Insr
R2648	T4254	T4229	punct	.,map
R2649	T4256	T4257	det	The,region
R2650	T4257	T4258	nsubjpass	region,covered
R2651	T4259	T4258	auxpass	is,covered
R2652	T4260	T4258	agent	by,covered
R2653	T4261	T4262	nummod	three,BACs
R2654	T4262	T4260	pobj	BACs,by
R2655	T4263	T4262	punct	: ,BACs
R2656	T4264	T4265	compound	RPCI,23_334D24
R2657	T4265	T4262	appos	23_334D24,BACs
R2658	T4266	T4265	punct	-,23_334D24
R2659	T4267	T4268	punct	(,AC087153
R2660	T4268	T4265	parataxis	AC087153,23_334D24
R2661	T4269	T4268	punct	),AC087153
R2662	T4270	T4265	punct	", ",23_334D24
R2663	T4271	T4272	compound	RPCI,23_312B8
R2664	T4272	T4265	conj	23_312B8,23_334D24
R2665	T4273	T4272	punct	-,23_312B8
R2666	T4274	T4275	punct	(,AC087150
R2667	T4275	T4272	parataxis	AC087150,23_312B8
R2668	T4276	T4275	punct	),AC087150
R2669	T4277	T4272	punct	", ",23_312B8
R2670	T4278	T4272	cc	and,23_312B8
R2671	T4279	T4280	compound	RPCI,23_387H4
R2672	T4280	T4272	conj	23_387H4,23_312B8
R2673	T4281	T4280	punct	-,23_387H4
R2674	T4282	T4283	punct	(,AC079544
R2675	T4283	T4280	parataxis	AC079544,23_387H4
R2676	T4284	T4283	punct	),AC079544
R2677	T4285	T4258	punct	.,covered
R2678	T4287	T4288	punct	(,B
R2679	T4288	T4289	meta	B,shows
R2680	T4290	T4288	punct	),B
R2681	T4291	T4292	det	The,map
R2682	T4292	T4289	nsubj	map,shows
R2683	T4293	T4292	compound	transcript,map
R2684	T4294	T4292	prep	of,map
R2685	T4295	T4294	pobj	Mcoln1,of
R2686	T4296	T4297	nummod	14,exons
R2687	T4297	T4289	dobj	exons,shows
R2688	T4298	T4289	punct	", ",shows
R2689	T4299	T4289	cc	and,shows
R2690	T4300	T4301	det	the,locations
R2691	T4301	T4302	nsubjpass	locations,illustrated
R2692	T4302	T4289	conj	illustrated,shows
R2693	T4303	T4301	prep	of,locations
R2694	T4304	T4305	det	the,probes
R2695	T4305	T4303	pobj	probes,of
R2696	T4306	T4305	acl	used,probes
R2697	T4307	T4306	prep	in,used
R2698	T4308	T4309	det	this,study
R2699	T4309	T4307	pobj	study,in
R27	T145	T139	attr	disorder,is
R270	T560	T561	compound	Mucolipidosis,type
R2700	T4310	T4302	auxpass	are,illustrated
R2701	T4311	T4302	punct	.,illustrated
R2702	T4313	T4314	det	The,map
R2703	T4314	T4315	nsubj	map,shows
R2704	T4316	T4315	advmod	also,shows
R2705	T4317	T4318	det	the,exon
R2706	T4318	T4315	dobj	exon,shows
R2707	T4319	T4318	amod	expanded,exon
R2708	T4320	T4318	nummod	13,exon
R2709	T4321	T4315	prep	in,shows
R271	T561	T562	nsubj	type,is
R2710	T4322	T4323	det	the,transcript
R2711	T4323	T4321	pobj	transcript,in
R2712	T4324	T4325	advmod	alternatively,spliced
R2713	T4325	T4323	amod	spliced,transcript
R2714	T4326	T4327	punct	(,box
R2715	T4327	T4315	parataxis	box,shows
R2716	T4328	T4327	amod	hashed,box
R2717	T4329	T4327	punct	),box
R2718	T4330	T4315	punct	.,shows
R2719	T4332	T4333	compound	UniGene,Mm.39099
R272	T563	T561	nummod	IV,type
R2720	T4333	T4335	nsubj	Mm.39099,is
R2721	T4334	T4333	compound	cluster,Mm.39099
R2722	T4336	T4337	det	the,homologue
R2723	T4337	T4335	attr	homologue,is
R2724	T4338	T4337	prep	of,homologue
R2725	T4339	T4340	det	the,gene
R2726	T4340	T4338	pobj	gene,of
R2727	T4341	T4340	amod	human,gene
R2728	T4342	T4340	compound	zinc,gene
R2729	T4343	T4340	compound	finger,gene
R273	T564	T561	punct	(,type
R2730	T4344	T4345	punct	(,AC001252
R2731	T4345	T4335	parataxis	AC001252,is
R2732	T4346	T4345	punct	),AC001252
R2733	T4347	T4335	punct	.,is
R2734	T4349	T4350	nsubjpass	It,noted
R2735	T4351	T4350	aux	should,noted
R2736	T4352	T4350	auxpass	be,noted
R2737	T4353	T4354	mark	that,is
R2738	T4354	T4350	ccomp	is,noted
R2739	T4355	T4356	det	the,scale
R274	T565	T561	appos	MLIV,type
R2740	T4356	T4354	nsubj	scale,is
R2741	T4357	T4356	prep	of,scale
R2742	T4358	T4359	det	the,map
R2743	T4359	T4357	pobj	map,of
R2744	T4360	T4359	compound	transcript,map
R2745	T4361	T4354	prep	in,is
R2746	T4362	T4361	pobj	reference,in
R2747	T4363	T4362	prep	to,reference
R2748	T4364	T4365	det	the,scale
R2749	T4365	T4363	pobj	scale,to
R275	T566	T567	punct	;,MIM
R2750	T4366	T4367	nummod	201,kb
R2751	T4367	T4365	compound	kb,scale
R2752	T4368	T4365	prep	of,scale
R2753	T4369	T4370	compound	BAC,23_387H4
R2754	T4370	T4368	pobj	23_387H4,of
R2755	T4371	T4370	compound	RPCI,23_387H4
R2756	T4372	T4370	punct	-,23_387H4
R2757	T4373	T4350	punct	.,noted
R276	T567	T561	parataxis	MIM,type
R2761	T4431	T4432	compound	Peptide,sequence
R2762	T4432	T4433	compound	sequence,comparison
R2763	T4434	T4433	prep	of,comparison
R2764	T4435	T4434	pobj	Mcoln1,of
R2765	T4436	T4433	prep	to,comparison
R2766	T4437	T4438	nmod	human,homologues
R2767	T4438	T4436	pobj	homologues,to
R2768	T4439	T4437	punct	", ",human
R2769	T4440	T4441	compound	D.,melanogaster
R277	T568	T567	nummod	252650,MIM
R2770	T4441	T4437	conj	melanogaster,human
R2771	T4442	T4441	punct	", ",melanogaster
R2772	T4443	T4441	cc	and,melanogaster
R2773	T4444	T4445	compound	C.,elegans
R2774	T4445	T4441	conj	elegans,melanogaster
R2775	T4446	T4445	punct	(,elegans
R2776	T4447	T4448	compound	cup,5
R2777	T4448	T4445	appos	5,elegans
R2778	T4449	T4448	punct	-,5
R2779	T4450	T4438	punct	),homologues
R278	T569	T567	punct	),MIM
R2780	T4451	T4433	punct	.,comparison
R2781	T4453	T4454	amod	Blue,lines
R2782	T4454	T4455	nsubj	lines,indicate
R2783	T4455	T4456	ccomp	indicate,surrounds
R2784	T4457	T4458	compound	transmembrane,domains
R2785	T4458	T4455	dobj	domains,indicate
R2786	T4459	T4456	punct	", ",surrounds
R2787	T4460	T4461	det	the,box
R2788	T4461	T4463	nsubj	box,surrounds
R2789	T4462	T4461	amod	red,box
R279	T570	T571	det	an,disorder
R2790	T4463	T4456	ccomp	surrounds,surrounds
R2791	T4464	T4465	det	the,pore
R2792	T4465	T4463	dobj	pore,surrounds
R2793	T4466	T4465	amod	putative,pore
R2794	T4467	T4465	compound	channel,pore
R2795	T4468	T4456	punct	", ",surrounds
R2796	T4469	T4470	det	the,box
R2797	T4470	T4456	nsubj	box,surrounds
R2798	T4471	T4470	compound	orange,box
R2799	T4472	T4473	det	the,signal
R28	T146	T145	amod	autosomal,disorder
R280	T571	T562	attr	disorder,is
R2800	T4473	T4456	dobj	signal,surrounds
R2801	T4474	T4473	amod	putative,signal
R2802	T4475	T4476	amod	late,targeting
R2803	T4476	T4473	compound	targeting,signal
R2804	T4477	T4478	amod	endosomal,lysosomal
R2805	T4478	T4476	amod	lysosomal,targeting
R2806	T4479	T4478	punct	/,lysosomal
R2807	T4480	T4456	punct	.,surrounds
R2809	T4522	T4523	compound	Northern,analysis
R281	T572	T571	amod	autosomal,disorder
R2810	T4524	T4523	prep	of,analysis
R2811	T4525	T4524	pobj	Mcoln1,of
R2812	T4526	T4523	punct	.,analysis
R2813	T4528	T4529	punct	(,A
R2814	T4529	T4530	meta	A,Northern
R2815	T4530	T4535	nmod	Northern,blots
R2816	T4531	T4529	punct	),A
R2817	T4532	T4533	nmod	Mouse,tissue
R2818	T4533	T4530	nmod	tissue,Northern
R2819	T4534	T4533	amod	fetal,tissue
R282	T573	T571	amod	recessive,disorder
R2820	T4535	T4544	nsubj	blots,hybridized
R2821	T4536	T4530	cc	and,Northern
R2822	T4537	T4538	punct	(,B
R2823	T4538	T4539	meta	B,Northern
R2824	T4539	T4530	conj	Northern,Northern
R2825	T4540	T4538	punct	),B
R2826	T4541	T4542	nmod	mouse,tissue
R2827	T4542	T4539	compound	tissue,Northern
R2828	T4543	T4542	amod	multiple,tissue
R2829	T4545	T4546	punct	(,Clontech
R283	T574	T571	amod	lysosomal,disorder
R2830	T4546	T4535	parataxis	Clontech,blots
R2831	T4547	T4546	punct	),Clontech
R2832	T4548	T4544	prep	with,hybridized
R2833	T4549	T4550	det	a,probe
R2834	T4550	T4548	pobj	probe,with
R2835	T4551	T4550	acl	generated,probe
R2836	T4552	T4551	prep	from,generated
R2837	T4553	T4552	pobj	exon,from
R2838	T4554	T4553	nummod	2,exon
R2839	T4555	T4556	punct	(,1B
R284	T575	T571	compound	storage,disorder
R2840	T4556	T4544	parataxis	1B,hybridized
R2841	T4557	T4556	dep	probe,1B
R2842	T4558	T4557	nummod	1,probe
R2843	T4559	T4556	punct	", ",1B
R2844	T4560	T4556	compound	Fig.,1B
R2845	T4561	T4556	punct	),1B
R2846	T4562	T4544	punct	.,hybridized
R2847	T4564	T4565	punct	(,C
R2848	T4565	T4566	meta	C,Hybridization
R2849	T4567	T4565	punct	),C
R285	T576	T577	dep	that,characterized
R2850	T4568	T4566	prep	of,Hybridization
R2851	T4569	T4570	det	the,blot
R2852	T4570	T4568	pobj	blot,of
R2853	T4571	T4572	amod	multiple,tissue
R2854	T4572	T4570	compound	tissue,blot
R2855	T4573	T4566	prep	with,Hybridization
R2856	T4574	T4575	det	a,probe
R2857	T4575	T4573	pobj	probe,with
R2858	T4576	T4575	prep	from,probe
R2859	T4577	T4578	det	the,segment
R286	T577	T571	relcl	characterized,disorder
R2860	T4578	T4576	pobj	segment,from
R2861	T4579	T4580	advmod	alternatively,spliced
R2862	T4580	T4578	amod	spliced,segment
R2863	T4581	T4578	prep	of,segment
R2864	T4582	T4581	pobj	exon,of
R2865	T4583	T4582	nummod	13,exon
R2866	T4584	T4585	punct	(,1B
R2867	T4585	T4566	parataxis	1B,Hybridization
R2868	T4586	T4585	dep	probe,1B
R2869	T4587	T4586	nummod	2,probe
R287	T578	T577	auxpass	is,characterized
R2870	T4588	T4585	punct	", ",1B
R2871	T4589	T4585	compound	Fig.,1B
R2872	T4590	T4585	punct	),1B
R2873	T4591	T4566	punct	.,Hybridization
R2874	T4593	T4594	compound	β,Actin
R2875	T4594	T4596	compound	Actin,hybridization
R2876	T4595	T4594	punct	-,Actin
R2877	T4596	T4598	nsubjpass	hybridization,shown
R2878	T4597	T4596	compound	control,hybridization
R2879	T4599	T4598	auxpass	is,shown
R288	T579	T577	agent	by,characterized
R2880	T4600	T4598	prep	below,shown
R2881	T4601	T4602	det	each,blot
R2882	T4602	T4600	pobj	blot,below
R2883	T4603	T4598	punct	.,shown
R2884	T4631	T4632	compound	Peptide,sequence
R2885	T4632	T4633	compound	sequence,comparison
R2886	T4634	T4633	prep	of,comparison
R2887	T4635	T4636	det	the,isoforms
R2888	T4636	T4634	pobj	isoforms,of
R2889	T4637	T4636	nummod	two,isoforms
R289	T580	T581	amod	corneal,clouding
R2890	T4638	T4639	advmod	alternatively,spliced
R2891	T4639	T4636	amod	spliced,isoforms
R2892	T4640	T4636	compound	Mcoln1,isoforms
R2893	T4641	T4633	punct	.,comparison
R2894	T4643	T4644	det	The,box
R2895	T4644	T4646	nsubj	box,surrounds
R2896	T4645	T4644	amod	green,box
R2897	T4647	T4648	det	the,tails
R2898	T4648	T4646	dobj	tails,surrounds
R2899	T4649	T4648	amod	divergent,tails
R29	T147	T145	amod	recessive,disorder
R290	T581	T579	pobj	clouding,by
R2900	T4650	T4651	npadvmod	c,terminal
R2901	T4651	T4648	amod	terminal,tails
R2902	T4652	T4651	punct	-,terminal
R2903	T4653	T4648	amod	cytoplasmic,tails
R2904	T4654	T4646	punct	.,surrounds
R2905	T4656	T4657	det	The,lines
R2906	T4657	T4659	nsubj	lines,indicate
R2907	T4658	T4657	amod	blue,lines
R2908	T4660	T4661	det	the,domains
R2909	T4661	T4659	dobj	domains,indicate
R291	T582	T581	punct	", ",clouding
R2910	T4662	T4661	compound	transmembrane,domains
R2911	T4663	T4659	punct	.,indicate
R292	T583	T584	amod	delayed,development
R293	T584	T581	conj	development,clouding
R294	T585	T584	compound	psychomotor,development
R295	T586	T584	punct	", ",development
R296	T587	T584	cc	and,development
R297	T588	T589	amod	mental,retardation
R298	T589	T584	conj	retardation,development
R299	T590	T591	dep	that,presents
R30	T148	T145	amod	lysosomal,disorder
R300	T591	T589	relcl	presents,retardation
R301	T592	T591	advmod	usually,presents
R302	T593	T591	prep	during,presents
R303	T594	T595	det	the,year
R304	T595	T593	pobj	year,during
R305	T596	T595	amod	first,year
R306	T597	T595	prep	of,year
R307	T598	T597	pobj	life,of
R308	T599	T600	punct	[,1
R309	T600	T562	parataxis	1,is
R31	T149	T145	compound	storage,disorder
R310	T601	T600	punct	],1
R311	T602	T562	punct	.,is
R312	T604	T605	det	Another,characteristic
R313	T605	T608	nsubj	characteristic,is
R314	T606	T605	amod	interesting,characteristic
R315	T607	T605	amod	clinical,characteristic
R316	T609	T610	mark	that,are
R317	T610	T608	ccomp	are,is
R318	T611	T610	nsubj	patients,are
R319	T612	T613	advmod	constitutively,achlorhydric
R32	T150	T145	acl	characterized,disorder
R320	T613	T610	acomp	achlorhydric,are
R321	T614	T613	prep	with,achlorhydric
R322	T615	T616	amod	associated,hypergastremia
R323	T616	T614	pobj	hypergastremia,with
R324	T617	T618	punct	[,2
R325	T618	T608	parataxis	2,is
R326	T619	T618	punct	],2
R327	T620	T608	punct	.,is
R328	T622	T623	nsubj	Patients,show
R329	T624	T622	prep	with,Patients
R33	T151	T150	agent	by,characterized
R330	T625	T624	pobj	MLIV,with
R331	T626	T623	aux	do,show
R332	T627	T623	neg	not,show
R333	T628	T629	compound	mucopolysaccharide,excretion
R334	T629	T623	dobj	excretion,show
R335	T630	T629	punct	", ",excretion
R336	T631	T632	amod	skeletal,changes
R337	T632	T629	conj	changes,excretion
R338	T633	T632	punct	", ",changes
R339	T634	T632	cc	or,changes
R34	T152	T153	amod	severe,abnormalities
R340	T635	T632	conj	organomegaly,changes
R341	T636	T623	prep	like,show
R342	T637	T638	det	the,mucolipidoses
R343	T638	T636	pobj	mucolipidoses,like
R344	T639	T638	amod	other,mucolipidoses
R345	T640	T623	punct	.,show
R346	T642	T643	amod	Abnormal,bodies
R347	T643	T646	nsubjpass	bodies,found
R348	T644	T643	amod	lysosomal,bodies
R349	T645	T643	compound	storage,bodies
R35	T153	T151	pobj	abnormalities,by
R350	T647	T643	cc	and,bodies
R351	T648	T649	amod	large,vacuoles
R352	T649	T643	conj	vacuoles,bodies
R353	T650	T646	aux	have,found
R354	T651	T646	auxpass	been,found
R355	T652	T646	prep	in,found
R356	T653	T654	nmod	skin,biopsies
R357	T654	T652	pobj	biopsies,in
R358	T655	T653	cc	and,skin
R359	T656	T653	conj	conjuctival,skin
R36	T154	T153	amod	neurologic,abnormalities
R360	T657	T646	advcl	using,found
R361	T658	T659	compound	electron,microscopy
R362	T659	T657	dobj	microscopy,using
R363	T660	T659	punct	-,microscopy
R364	T661	T646	cc	and,found
R365	T662	T646	punct	", ",found
R366	T663	T664	amod	prior,to
R367	T664	T646	prep	to,found
R368	T665	T666	compound	gene,identification
R369	T666	T664	pobj	identification,to
R37	T155	T154	cc	and,neurologic
R370	T667	T646	punct	", ",found
R371	T668	T646	conj	served,found
R372	T669	T668	prep	as,served
R373	T670	T671	det	the,means
R374	T671	T669	pobj	means,as
R375	T672	T671	amod	only,means
R376	T673	T671	prep	of,means
R377	T674	T673	pobj	diagnosis,of
R378	T675	T676	punct	[,3
R379	T676	T668	parataxis	3,served
R38	T156	T154	conj	ophthalmologic,neurologic
R380	T677	T678	punct	-,5
R381	T678	T676	prep	5,3
R382	T679	T676	punct	],3
R383	T680	T646	punct	.,found
R384	T682	T683	det	A,report
R385	T683	T685	nsubj	report,estimates
R386	T684	T683	amod	recent,report
R387	T686	T687	mark	that,is
R388	T687	T685	ccomp	is,estimates
R389	T688	T689	det	the,frequency
R39	T157	T139	punct	.,is
R390	T689	T687	nsubj	frequency,is
R391	T690	T689	compound	carrier,frequency
R392	T691	T689	prep	of,frequency
R393	T692	T691	pobj	MLIV,of
R394	T693	T689	prep	in,frequency
R395	T694	T695	det	the,population
R396	T695	T693	pobj	population,in
R397	T696	T697	npadvmod	Ashkenazi,Jewish
R398	T697	T695	amod	Jewish,population
R399	T698	T687	attr	1,is
R4	T115	T116	nsubj	Cloning,reveals
R40	T159	T160	advmod	Recently,identified
R400	T699	T698	prep	in,1
R401	T700	T699	pobj	100,in
R402	T701	T685	punct	", ",estimates
R403	T702	T685	cc	and,estimates
R404	T703	T704	nsubjpass	mutations,reported
R405	T704	T685	conj	reported,estimates
R406	T705	T704	aux	have,reported
R407	T706	T704	auxpass	been,reported
R408	T707	T704	prep	in,reported
R409	T708	T709	amod	Jewish,families
R41	T161	T162	det	the,gene
R410	T709	T707	pobj	families,in
R411	T710	T708	cc	and,Jewish
R412	T711	T708	conj	non-Jewish,Jewish
R413	T712	T713	punct	[,6
R414	T713	T704	parataxis	6,reported
R415	T714	T715	punct	-,9
R416	T715	T713	prep	9,6
R417	T716	T713	punct	],6
R418	T717	T704	punct	.,reported
R419	T719	T720	det	The,gene
R42	T162	T160	nsubjpass	gene,identified
R420	T720	T722	nsubj	gene,maps
R421	T721	T720	amod	human,gene
R422	T723	T720	appos	MCOLN1,gene
R423	T724	T725	punct	(,AF287270
R424	T725	T720	parataxis	AF287270,gene
R425	T726	T725	compound	GenBank,AF287270
R426	T727	T725	compound	#,AF287270
R427	T728	T725	punct	),AF287270
R428	T729	T722	prep	to,maps
R429	T730	T729	pobj	chromosome,to
R43	T163	T162	compound	MLIV,gene
R430	T731	T730	nummod	19p13.2,chromosome
R431	T732	T733	punct	-,13.3
R432	T733	T730	prep	13.3,chromosome
R433	T734	T722	cc	and,maps
R434	T735	T722	conj	encodes,maps
R435	T736	T737	det	a,protein
R436	T737	T735	dobj	protein,encodes
R437	T738	T737	amod	novel,protein
R438	T739	T740	dep	that,is
R439	T740	T737	relcl	is,protein
R44	T164	T162	punct	", ",gene
R440	T741	T742	det	a,member
R441	T742	T740	attr	member,is
R442	T743	T742	prep	of,member
R443	T744	T745	det	the,superfamily
R444	T745	T743	pobj	superfamily,of
R445	T746	T747	amod	transient,potential
R446	T747	T745	nmod	potential,superfamily
R447	T748	T747	nmod	receptor,potential
R448	T749	T747	punct	(,potential
R449	T750	T747	appos	TRP,potential
R45	T165	T162	appos	MCOLN1,gene
R450	T751	T745	punct	),superfamily
R451	T752	T753	compound	cation,channel
R452	T753	T745	compound	channel,superfamily
R453	T754	T745	compound	gene,superfamily
R454	T755	T756	punct	[,7
R455	T756	T735	parataxis	7,encodes
R456	T757	T758	punct	-,10
R457	T758	T756	prep	10,7
R458	T759	T756	punct	],7
R459	T760	T722	punct	.,maps
R46	T166	T160	punct	", ",identified
R460	T762	T763	compound	Protein,trafficking
R461	T763	T764	compound	trafficking,studies
R462	T764	T765	nsubj	studies,suggest
R463	T766	T767	mark	that,is
R464	T767	T765	ccomp	is,suggest
R465	T768	T767	nsubj	MLIV,is
R466	T769	T770	det	the,result
R467	T770	T767	attr	result,is
R468	T771	T770	prep	of,result
R469	T772	T773	det	a,defect
R47	T167	T160	aux	has,identified
R470	T773	T771	pobj	defect,of
R471	T774	T773	prep	in,defect
R472	T775	T776	det	the,pathway
R473	T776	T774	pobj	pathway,in
R474	T777	T776	amod	late,pathway
R475	T778	T776	amod	endocytic,pathway
R476	T779	T765	punct	", ",suggest
R477	T780	T765	advcl	contrary,suggest
R478	T781	T780	prep	to,contrary
R479	T782	T783	det	the,mucolipidoses
R48	T168	T160	auxpass	been,identified
R480	T783	T781	pobj	mucolipidoses,to
R481	T784	T783	amod	other,mucolipidoses
R482	T785	T786	dep	which,caused
R483	T786	T783	relcl	caused,mucolipidoses
R484	T787	T786	auxpass	are,caused
R485	T788	T786	advmod	typically,caused
R486	T789	T786	agent	by,caused
R487	T790	T791	amod	defective,hydrolases
R488	T791	T789	pobj	hydrolases,by
R489	T792	T791	amod	lysosomal,hydrolases
R49	T169	T160	prep	as,identified
R490	T793	T794	punct	[,12
R491	T794	T765	parataxis	12,suggest
R492	T795	T794	nummod	11,12
R493	T796	T794	punct	",",12
R494	T797	T794	punct	],12
R495	T798	T765	punct	.,suggest
R496	T800	T801	amod	Recent,work
R497	T801	T802	nsubj	work,supports
R498	T803	T801	prep	in,work
R499	T804	T805	compound	Caenorhabditis,elegans
R5	T118	T115	cc	and,Cloning
R50	T170	T171	det	a,member
R500	T805	T803	pobj	elegans,in
R501	T806	T807	det	this,hypothesis
R502	T807	T802	dobj	hypothesis,supports
R503	T808	T802	punct	.,supports
R504	T810	T811	nmod	Loss,mutants
R505	T811	T814	nsubj	mutants,result
R506	T812	T810	prep	of,Loss
R507	T813	T812	pobj	function,of
R508	T815	T811	prep	of,mutants
R509	T816	T817	det	the,homologue
R51	T171	T169	pobj	member,as
R510	T817	T815	pobj	homologue,of
R511	T818	T819	compound	MCOLN1,elegans
R512	T819	T817	compound	elegans,homologue
R513	T820	T819	compound	C.,elegans
R514	T821	T817	punct	", ",homologue
R515	T822	T817	appos	cup,homologue
R516	T823	T822	punct	-,cup
R517	T824	T822	nummod	5,cup
R518	T825	T814	punct	", ",result
R519	T826	T814	prep	in,result
R52	T172	T171	amod	new,member
R520	T827	T828	det	an,rate
R521	T828	T826	pobj	rate,in
R522	T829	T828	amod	increased,rate
R523	T830	T828	prep	of,rate
R524	T831	T830	pobj	endocytosis,of
R525	T832	T828	punct	", ",rate
R526	T833	T828	conj	accumulation,rate
R527	T834	T833	prep	of,accumulation
R528	T835	T836	amod	large,vacuoles
R529	T836	T834	pobj	vacuoles,of
R53	T173	T171	prep	of,member
R530	T837	T833	punct	", ",accumulation
R531	T838	T833	cc	and,accumulation
R532	T839	T840	det	a,rate
R533	T840	T833	conj	rate,accumulation
R534	T841	T840	amod	decreased,rate
R535	T842	T840	prep	of,rate
R536	T843	T844	amod	endocytosed,protein
R537	T844	T845	compound	protein,breakdown
R538	T845	T842	pobj	breakdown,of
R539	T846	T814	punct	;,result
R54	T174	T175	det	the,superfamily
R540	T847	T848	mark	while,reverses
R541	T848	T814	advcl	reverses,result
R542	T849	T848	nsubj	over-expression,reverses
R543	T850	T849	prep	of,over-expression
R544	T851	T852	det	this,gene
R545	T852	T850	pobj	gene,of
R546	T853	T854	det	the,phenotype
R547	T854	T848	dobj	phenotype,reverses
R548	T855	T856	punct	[,13
R549	T856	T814	parataxis	13,result
R55	T175	T173	pobj	superfamily,of
R550	T857	T856	punct	],13
R551	T858	T814	punct	.,result
R552	T860	T861	nsubj	Cloning,is
R553	T862	T860	cc	and,Cloning
R554	T863	T860	conj	characterization,Cloning
R555	T864	T860	prep	of,Cloning
R556	T865	T866	det	the,homologue
R557	T866	T864	pobj	homologue,of
R558	T867	T866	compound	mouse,homologue
R559	T868	T866	prep	of,homologue
R56	T176	T177	amod	transient,potential
R560	T869	T868	pobj	MCOLN1,of
R561	T870	T861	acomp	crucial,is
R562	T871	T861	prep	for,is
R563	T872	T873	det	the,development
R564	T873	T871	pobj	development,for
R565	T874	T873	prep	of,development
R566	T875	T876	compound	mouse,models
R567	T876	T874	pobj	models,of
R568	T877	T876	prep	of,models
R569	T878	T877	pobj	MLIV,of
R57	T177	T175	nmod	potential,superfamily
R570	T879	T880	aux	to,study
R571	T880	T861	advcl	study,is
R572	T881	T880	advmod	further,study
R573	T882	T883	det	this,disorder
R574	T883	T880	dobj	disorder,study
R575	T884	T861	punct	.,is
R579	T1036	T1035	cc	and,Cloning
R58	T178	T177	nmod	receptor,potential
R580	T1037	T1035	conj	mapping,Cloning
R581	T1038	T1035	prep	of,Cloning
R582	T1039	T1040	det	the,homologue
R583	T1040	T1038	pobj	homologue,of
R584	T1041	T1040	compound	mouse,homologue
R585	T1042	T1040	appos	Mcoln1,homologue
R586	T1044	T1045	prep	In,compared
R587	T1046	T1044	pobj	order,In
R588	T1047	T1048	aux	to,clone
R589	T1048	T1046	acl	clone,order
R59	T179	T177	punct	(,potential
R590	T1049	T1050	det	the,homologue
R591	T1050	T1048	dobj	homologue,clone
R592	T1051	T1050	compound	mouse,homologue
R593	T1052	T1050	prep	of,homologue
R594	T1053	T1052	pobj	MCOLN1,of
R595	T1054	T1045	punct	", ",compared
R596	T1055	T1056	det	the,sequence
R597	T1056	T1045	nsubjpass	sequence,compared
R598	T1057	T1056	amod	human,sequence
R599	T1058	T1059	compound	amino,acid
R6	T119	T115	conj	characterization,Cloning
R60	T180	T177	appos	TRP,potential
R600	T1059	T1056	compound	acid,sequence
R601	T1060	T1045	auxpass	was,compared
R602	T1061	T1045	prep	to,compared
R603	T1062	T1063	det	the,database
R604	T1063	T1061	pobj	database,to
R605	T1064	T1065	amod	high,throughput
R606	T1065	T1066	nmod	throughput,sequence
R607	T1066	T1063	nmod	sequence,database
R608	T1067	T1066	amod	genomic,sequence
R609	T1068	T1066	punct	(,sequence
R61	T181	T175	punct	),superfamily
R610	T1069	T1066	appos	HTGS,sequence
R611	T1070	T1063	punct	),database
R612	T1071	T1045	advcl	using,compared
R613	T1072	T1071	dobj	TBLASTN,using
R614	T1073	T1045	punct	", ",compared
R615	T1074	T1075	dep	which,identified
R616	T1075	T1045	advcl	identified,compared
R617	T1076	T1077	det	the,clone
R618	T1077	T1075	dobj	clone,identified
R619	T1078	T1079	compound	mouse,BAC
R62	T182	T183	compound	cation,channel
R620	T1079	T1077	compound	BAC,clone
R621	T1080	T1081	compound	RPCI,23_387H4
R622	T1081	T1077	appos	23_387H4,clone
R623	T1082	T1081	punct	-,23_387H4
R624	T1083	T1084	punct	(,AC079544.1
R625	T1084	T1077	parataxis	AC079544.1,clone
R626	T1085	T1084	compound	GB,AC079544.1
R627	T1086	T1084	compound	No.,AC079544.1
R628	T1087	T1084	punct	),AC079544.1
R629	T1088	T1045	punct	.,compared
R63	T183	T175	compound	channel,superfamily
R630	T1090	T1091	nsubj	Correspondence,confirmed
R631	T1092	T1090	prep	with,Correspondence
R632	T1093	T1094	det	the,Institute
R633	T1094	T1092	pobj	Institute,with
R634	T1095	T1094	compound	Joint,Institute
R635	T1096	T1094	compound	Genome,Institute
R636	T1097	T1094	cc	and,Institute
R637	T1098	T1099	det	the,Laboratory
R638	T1099	T1103	nmod	Laboratory,Center
R639	T1100	T1101	nmod	Lawrence,Livermore
R64	T184	T160	punct	.,identified
R640	T1101	T1099	nmod	Livermore,Laboratory
R641	T1102	T1099	nmod	National,Laboratory
R642	T1103	T1094	conj	Center,Institute
R643	T1104	T1099	punct	(,Laboratory
R644	T1105	T1099	appos	LLNL,Laboratory
R645	T1106	T1103	punct	),Center
R646	T1107	T1108	compound	Human,Genome
R647	T1108	T1103	compound	Genome,Center
R648	T1109	T1110	det	the,location
R649	T1110	T1091	dobj	location,confirmed
R65	T186	T187	advmod	Here,report
R650	T1111	T1110	prep	of,location
R651	T1112	T1113	det	this,BAC
R652	T1113	T1111	pobj	BAC,of
R653	T1114	T1110	prep	to,location
R654	T1115	T1116	compound	mouse,chromosome
R655	T1116	T1114	pobj	chromosome,to
R656	T1117	T1116	nummod	8,chromosome
R657	T1118	T1091	cc	and,confirmed
R658	T1119	T1091	conj	allowed,confirmed
R659	T1120	T1121	nsubj	us,construct
R66	T188	T187	nsubj	we,report
R660	T1121	T1119	ccomp	construct,allowed
R661	T1122	T1121	aux	to,construct
R662	T1123	T1124	det	a,map
R663	T1124	T1121	dobj	map,construct
R664	T1125	T1124	amod	physical,map
R665	T1126	T1124	prep	of,map
R666	T1127	T1128	det	this,region
R667	T1128	T1126	pobj	region,of
R668	T1129	T1130	punct	[,14
R669	T1130	T1119	parataxis	14,allowed
R67	T189	T190	det	the,cloning
R670	T1131	T1130	punct	],14
R671	T1132	T1133	punct	(,1A
R672	T1133	T1119	parataxis	1A,allowed
R673	T1134	T1133	compound	Fig.,1A
R674	T1135	T1133	punct	),1A
R675	T1136	T1091	punct	.,confirmed
R676	T1138	T1139	det	The,sequence
R677	T1139	T1141	nsubjpass	sequence,compared
R678	T1140	T1139	compound	BAC,sequence
R679	T1142	T1141	auxpass	was,compared
R68	T190	T187	dobj	cloning,report
R680	T1143	T1141	advmod	then,compared
R681	T1144	T1141	prep	to,compared
R682	T1145	T1146	det	the,database
R683	T1146	T1144	pobj	database,to
R684	T1147	T1148	compound	mouse,EST
R685	T1148	T1146	compound	EST,database
R686	T1149	T1141	advcl	using,compared
R687	T1150	T1149	dobj	BLASTN,using
R688	T1151	T1141	punct	", ",compared
R689	T1152	T1141	cc	and,compared
R69	T191	T190	cc	and,cloning
R690	T1153	T1154	amod	multiple,ESTs
R691	T1154	T1155	nsubjpass	ESTs,identified
R692	T1155	T1141	conj	identified,compared
R693	T1156	T1154	cc	and,ESTs
R694	T1157	T1158	poss	their,clones
R695	T1158	T1154	conj	clones,ESTs
R696	T1159	T1158	amod	corresponding,clones
R697	T1160	T1158	compound	I.M.A.G.E.,clones
R698	T1161	T1155	auxpass	were,identified
R699	T1162	T1155	punct	.,identified
R7	T120	T115	prep	of,Cloning
R70	T192	T190	conj	characterization,cloning
R700	T1164	T1165	nummod	Three,clones
R701	T1165	T1166	nsubjpass	clones,obtained
R702	T1167	T1168	punct	(,1247566
R703	T1168	T1165	parataxis	1247566,clones
R704	T1169	T1168	nmod	ID,1247566
R705	T1170	T1168	nmod	Nos.,1247566
R706	T1171	T1168	nummod	604971,1247566
R707	T1172	T1168	punct	", ",1247566
R708	T1173	T1168	nummod	1228665,1247566
R709	T1174	T1168	punct	", ",1247566
R71	T193	T190	prep	of,cloning
R710	T1175	T1168	punct	),1247566
R711	T1176	T1165	prep	from,clones
R712	T1177	T1178	det	the,cluster
R713	T1178	T1176	pobj	cluster,from
R714	T1179	T1178	compound	UniGene,cluster
R715	T1180	T1178	appos	Mm.,cluster
R716	T1181	T1180	nummod	8356,Mm.
R717	T1182	T1166	auxpass	were,obtained
R718	T1183	T1166	punct	", ",obtained
R719	T1184	T1166	conj	sequenced,obtained
R72	T194	T195	det	the,homologue
R720	T1185	T1184	punct	", ",sequenced
R721	T1186	T1184	cc	and,sequenced
R722	T1187	T1184	conj	assembled,sequenced
R723	T1188	T1187	prep	into,assembled
R724	T1189	T1190	det	a,transcript
R725	T1190	T1188	pobj	transcript,into
R726	T1191	T1192	nummod	2,kb
R727	T1192	T1190	compound	kb,transcript
R728	T1193	T1190	prep	with,transcript
R729	T1194	T1195	det	an,frame
R73	T195	T193	pobj	homologue,of
R730	T1195	T1193	pobj	frame,with
R731	T1196	T1195	amod	open,frame
R732	T1197	T1195	compound	reading,frame
R733	T1198	T1195	prep	of,frame
R734	T1199	T1200	nummod	580,acids
R735	T1200	T1198	pobj	acids,of
R736	T1201	T1200	compound	amino,acids
R737	T1202	T1200	acl	designated,acids
R738	T1203	T1202	oprd	Mcoln1,designated
R739	T1204	T1205	punct	(,AF302010
R74	T196	T195	compound	mouse,homologue
R740	T1205	T1203	parataxis	AF302010,Mcoln1
R741	T1206	T1205	compound	GB,AF302010
R742	T1207	T1205	compound	No.,AF302010
R743	T1208	T1205	punct	),AF302010
R744	T1209	T1166	punct	.,obtained
R745	T1211	T1212	nsubj	Comparison,showed
R746	T1213	T1211	prep	of,Comparison
R747	T1214	T1215	det	the,sequence
R748	T1215	T1213	pobj	sequence,of
R749	T1216	T1215	compound	cDNA,sequence
R75	T197	T195	punct	", ",homologue
R750	T1217	T1211	prep	to,Comparison
R751	T1218	T1219	det	the,clone
R752	T1219	T1217	pobj	clone,to
R753	T1220	T1219	compound	BAC,clone
R754	T1221	T1222	mark	that,consists
R755	T1222	T1212	ccomp	consists,showed
R756	T1223	T1224	det	the,gene
R757	T1224	T1222	nsubj	gene,consists
R758	T1225	T1222	prep	of,consists
R759	T1226	T1227	nummod	14,exons
R76	T198	T195	appos	Mcoln1,homologue
R760	T1227	T1225	pobj	exons,of
R761	T1228	T1212	punct	.,showed
R762	T1230	T1231	nsubj	We,created
R763	T1232	T1231	advmod	then,created
R764	T1233	T1234	det	a,map
R765	T1234	T1231	dobj	map,created
R766	T1235	T1234	compound	transcript,map
R767	T1236	T1234	prep	of,map
R768	T1237	T1238	det	the,region
R769	T1238	T1236	pobj	region,of
R77	T199	T187	punct	", ",report
R770	T1239	T1238	compound	Mcoln1,region
R771	T1240	T1238	prep	of,region
R772	T1241	T1242	compound	mouse,chromosome
R773	T1242	T1240	pobj	chromosome,of
R774	T1243	T1242	nummod	8,chromosome
R775	T1244	T1245	punct	(,1B
R776	T1245	T1234	parataxis	1B,map
R777	T1246	T1245	compound	Fig.,1B
R778	T1247	T1245	punct	),1B
R779	T1248	T1231	punct	", ",created
R78	T200	T187	cc	and,report
R780	T1249	T1231	advcl	noting,created
R781	T1250	T1251	det	the,presence
R782	T1251	T1249	dobj	presence,noting
R783	T1252	T1251	prep	of,presence
R784	T1253	T1254	det	the,Cluster
R785	T1254	T1252	pobj	Cluster,of
R786	T1255	T1254	compound	UniGene,Cluster
R787	T1256	T1254	appos	Mm.,Cluster
R788	T1257	T1256	nummod	39099,Mm.
R789	T1258	T1254	punct	", ",Cluster
R79	T201	T187	conj	report,report
R790	T1259	T1260	det	a,homologue
R791	T1260	T1254	appos	homologue,Cluster
R792	T1261	T1260	prep	of,homologue
R793	T1262	T1263	det	the,gene
R794	T1263	T1261	pobj	gene,of
R795	T1264	T1263	amod	human,gene
R796	T1265	T1263	compound	zinc,gene
R797	T1266	T1263	compound	finger,gene
R798	T1267	T1268	punct	(,AC001252
R799	T1268	T1263	parataxis	AC001252,gene
R8	T121	T122	det	the,gene
R80	T202	T203	det	a,variant
R800	T1269	T1268	compound	GB,AC001252
R801	T1270	T1268	compound	No.,AC001252
R802	T1271	T1268	punct	),AC001252
R803	T1272	T1273	dep	that,terminates
R804	T1273	T1260	relcl	terminates,homologue
R805	T1274	T1275	advmod	approximately,1.8
R806	T1275	T1276	nummod	1.8,kb
R807	T1276	T1277	npadvmod	kb,before
R808	T1277	T1273	prep	before,terminates
R809	T1278	T1279	det	the,start
R81	T203	T201	dobj	variant,report
R810	T1279	T1277	pobj	start,before
R811	T1280	T1279	prep	of,start
R812	T1281	T1280	pobj	Mcoln1,of
R813	T1282	T1254	punct	;,Cluster
R814	T1283	T1254	cc	and,Cluster
R815	T1284	T1254	conj	Nte,Cluster
R816	T1285	T1284	punct	", ",Nte
R817	T1286	T1287	det	the,homologue
R818	T1287	T1284	appos	homologue,Nte
R819	T1288	T1287	compound	mouse,homologue
R82	T204	T203	amod	novel,variant
R820	T1289	T1287	prep	of,homologue
R821	T1290	T1291	det	the,gene
R822	T1291	T1289	pobj	gene,of
R823	T1292	T1291	amod	human,gene
R824	T1293	T1294	compound	neuropathy,esterase
R825	T1294	T1291	compound	esterase,gene
R826	T1295	T1294	compound	target,esterase
R827	T1296	T1291	punct	(,gene
R828	T1297	T1291	appos	NTE,gene
R829	T1298	T1284	punct	),Nte
R83	T205	T203	compound	splice,variant
R830	T1299	T1284	acl	beginning,Nte
R831	T1300	T1301	nummod	130,pairs
R832	T1301	T1303	npadvmod	pairs,after
R833	T1302	T1301	compound	base,pairs
R834	T1303	T1299	prep	after,beginning
R835	T1304	T1305	det	the,signal
R836	T1305	T1303	pobj	signal,after
R837	T1306	T1305	compound	polyadenylation,signal
R838	T1307	T1305	prep	for,signal
R839	T1308	T1307	pobj	Mcoln1,for
R84	T206	T207	dep	that,seen
R840	T1309	T1231	punct	.,created
R841	T1311	T1312	det	The,map
R842	T1312	T1314	nsubj	map,is
R843	T1313	T1312	compound	transcript,map
R844	T1315	T1312	prep	of,map
R845	T1316	T1317	det	the,region
R846	T1317	T1315	pobj	region,of
R847	T1318	T1317	acl	surrounding,region
R848	T1319	T1318	dobj	Mcoln1,surrounding
R849	T1320	T1314	acomp	similar,is
R85	T207	T203	relcl	seen,variant
R850	T1321	T1320	prep	to,similar
R851	T1322	T1323	det	the,region
R852	T1323	T1321	pobj	region,to
R853	T1324	T1323	amod	corresponding,region
R854	T1325	T1323	prep	of,region
R855	T1326	T1327	det	the,region
R856	T1327	T1325	pobj	region,of
R857	T1328	T1327	amod	homologous,region
R858	T1329	T1330	amod	human,MCOLN1
R859	T1330	T1327	compound	MCOLN1,region
R86	T208	T207	auxpass	is,seen
R860	T1331	T1332	punct	[,15
R861	T1332	T1314	parataxis	15,is
R862	T1333	T1332	punct	],15
R863	T1334	T1314	punct	", ",is
R864	T1335	T1314	cc	and,is
R865	T1336	T1337	det	the,presence
R866	T1337	T1338	nsubj	presence,extends
R867	T1338	T1314	conj	extends,is
R868	T1339	T1337	prep	of,presence
R869	T1340	T1341	det	the,gene
R87	T209	T207	neg	not,seen
R870	T1341	T1339	pobj	gene,of
R871	T1342	T1341	compound	zinc,gene
R872	T1343	T1341	compound	finger,gene
R873	T1344	T1341	cc	and,gene
R874	T1345	T1341	conj	Nte,gene
R875	T1346	T1338	dep	confirms,extends
R876	T1347	T1338	cc	and,extends
R877	T1348	T1349	det	the,region
R878	T1349	T1338	dobj	region,extends
R879	T1350	T1349	prep	of,region
R88	T210	T207	prep	in,seen
R880	T1351	T1350	pobj	synteny,of
R881	T1352	T1349	prep	between,region
R882	T1353	T1354	amod	human,chromosome
R883	T1354	T1352	pobj	chromosome,between
R884	T1355	T1354	nummod	19,chromosome
R885	T1356	T1354	cc	and,chromosome
R886	T1357	T1358	compound	mouse,chromosome
R887	T1358	T1354	conj	chromosome,chromosome
R888	T1359	T1358	nummod	8,chromosome
R889	T1360	T1338	punct	.,extends
R89	T211	T210	pobj	humans,in
R892	T1483	T1482	prep	of,Characterization
R893	T1484	T1483	pobj	Mcoln1,of
R894	T1486	T1487	nsubj	Comparison,showed
R895	T1488	T1486	prep	of,Comparison
R896	T1489	T1490	det	the,sequences
R897	T1490	T1488	pobj	sequences,of
R898	T1491	T1490	nmod	mouse,sequences
R899	T1492	T1491	cc	and,mouse
R9	T122	T120	pobj	gene,of
R90	T212	T187	punct	.,report
R900	T1493	T1491	conj	human,mouse
R901	T1494	T1490	compound	peptide,sequences
R902	T1495	T1496	nummod	91,%
R903	T1496	T1497	compound	%,identity
R904	T1497	T1487	dobj	identity,showed
R905	T1498	T1499	punct	(,Fig.
R906	T1499	T1487	parataxis	Fig.,showed
R907	T1500	T1499	nummod	2,Fig.
R908	T1501	T1499	punct	),Fig.
R909	T1502	T1487	punct	.,showed
R91	T216	T217	det	The,genes
R910	T1504	T1505	det	The,homologue
R911	T1505	T1508	nsubj	homologue,shows
R912	T1506	T1507	compound	C.,elegans
R913	T1507	T1505	compound	elegans,homologue
R914	T1509	T1505	appos	cup,homologue
R915	T1510	T1509	punct	-,cup
R916	T1511	T1509	nummod	5,cup
R917	T1512	T1513	nummod	34,%
R918	T1513	T1514	compound	%,identity
R919	T1514	T1508	dobj	identity,shows
R92	T217	T221	nsubj	genes,display
R920	T1515	T1514	prep	with,identity
R921	T1516	T1515	pobj	Mcoln1,with
R922	T1517	T1508	cc	and,shows
R923	T1518	T1519	compound	BLASTP,analysis
R924	T1519	T1520	nsubj	analysis,identified
R925	T1520	T1508	conj	identified,shows
R926	T1521	T1519	prep	of,analysis
R927	T1522	T1521	pobj	Mcoln1,of
R928	T1523	T1524	det	a,homologue
R929	T1524	T1520	dobj	homologue,identified
R93	T218	T219	amod	human,mouse
R930	T1525	T1524	amod	putative,homologue
R931	T1526	T1527	compound	Drosophila,melanogaster
R932	T1527	T1524	compound	melanogaster,homologue
R933	T1528	T1529	dep	that,shows
R934	T1529	T1524	relcl	shows,homologue
R935	T1530	T1531	nummod	38,%
R936	T1531	T1532	compound	%,identity
R937	T1532	T1529	dobj	identity,shows
R938	T1533	T1534	punct	(,Fig.
R939	T1534	T1520	parataxis	Fig.,identified
R94	T219	T217	compound	mouse,genes
R940	T1535	T1534	nummod	2,Fig.
R941	T1536	T1534	punct	),Fig.
R942	T1537	T1520	punct	.,identified
R943	T1539	T1540	advmod	Interestingly,occur
R944	T1541	T1540	punct	", ",occur
R945	T1542	T1543	nummod	two,substitutions
R946	T1543	T1540	nsubj	substitutions,occur
R947	T1544	T1543	compound	MCOLN1,substitutions
R948	T1545	T1546	compound	amino,acid
R949	T1546	T1543	compound	acid,substitutions
R95	T220	T219	cc	and,mouse
R950	T1547	T1548	dep	that,result
R951	T1548	T1543	relcl	result,substitutions
R952	T1549	T1548	prep	in,result
R953	T1550	T1549	pobj	MLIV,in
R954	T1551	T1540	prep	at,occur
R955	T1552	T1553	amod	conserved,acids
R956	T1553	T1551	pobj	acids,at
R957	T1554	T1553	compound	amino,acids
R958	T1555	T1540	punct	.,occur
R959	T1557	T1558	amod	TMPred,analysis
R96	T222	T223	det	a,degree
R960	T1558	T1559	nsubj	analysis,predicts
R961	T1560	T1561	det	a,structure
R962	T1561	T1559	dobj	structure,predicts
R963	T1562	T1561	compound	protein,structure
R964	T1563	T1564	dep	that,is
R965	T1564	T1561	relcl	is,structure
R966	T1565	T1566	advmod	nearly,identical
R967	T1566	T1564	acomp	identical,is
R968	T1567	T1566	prep	to,identical
R969	T1568	T1567	pobj	MCOLN1,to
R97	T223	T221	dobj	degree,display
R970	T1569	T1561	punct	", ",structure
R971	T1570	T1561	acl	containing,structure
R972	T1571	T1572	nummod	6,domains
R973	T1572	T1570	dobj	domains,containing
R974	T1573	T1572	compound	transmembrane,domains
R975	T1574	T1572	prep	with,domains
R976	T1575	T1576	det	the,termini
R977	T1576	T1574	pobj	termini,with
R978	T1577	T1576	nmod	N,termini
R979	T1578	T1577	punct	-,N
R98	T224	T223	amod	high,degree
R980	T1579	T1577	cc	and,N
R981	T1580	T1577	conj	C,N
R982	T1581	T1580	punct	-,C
R983	T1582	T1576	acl	residing,termini
R984	T1583	T1582	prep	in,residing
R985	T1584	T1585	det	the,cytoplasm
R986	T1585	T1583	pobj	cytoplasm,in
R987	T1586	T1587	punct	(,Fig.
R988	T1587	T1559	parataxis	Fig.,predicts
R989	T1588	T1587	nummod	2,Fig.
R99	T225	T223	prep	of,degree
R990	T1589	T1587	punct	),Fig.
R991	T1590	T1591	punct	[,9
R992	T1591	T1559	parataxis	9,predicts
R993	T1592	T1591	punct	],9
R994	T1593	T1559	punct	.,predicts
R997	T1674	T1675	compound	Expression,analysis
R998	T1676	T1675	prep	of,analysis
R999	T1677	T1676	pobj	Mcoln1,of

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PMC:88885 JSON TXT 8 Projects