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PMC:549712 / 57715-59846 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T16840 0-10 NN denotes Macrophage
T16842 11-23 NN denotes phagocytosis
T16841 24-30 NNS denotes assays
T16843 30-186 sentence denotes For preparation of monolayer cultures of macrophages, FLDMs were plated on glass coverslips in 24 well plates (2 × 105 cells per well) in X-Vivo 15 medium.
T16844 31-34 IN denotes For
T16846 35-46 NN denotes preparation
T16847 47-49 IN denotes of
T16848 50-59 NN denotes monolayer
T16849 60-68 NNS denotes cultures
T16850 69-71 IN denotes of
T16851 72-83 NNS denotes macrophages
T16852 83-85 , denotes ,
T16853 85-90 NNS denotes FLDMs
T16854 91-95 VBD denotes were
T16845 96-102 VBN denotes plated
T16855 103-105 IN denotes on
T16856 106-111 NN denotes glass
T16857 112-122 NNS denotes coverslips
T16858 123-125 IN denotes in
T16859 126-128 CD denotes 24
T16861 129-133 NN denotes well
T16860 134-140 NNS denotes plates
T16862 141-142 -LRB- denotes (
T16864 142-143 CD denotes 2
T16866 144-145 SYM denotes ×
T16865 146-149 CD denotes 105
T16863 150-155 NNS denotes cells
T16867 156-159 IN denotes per
T16868 160-164 NN denotes well
T16869 164-165 -RRB- denotes )
T16870 166-168 IN denotes in
T16871 169-170 NN denotes X
T16873 170-171 HYPH denotes -
T16872 171-175 NN denotes Vivo
T16875 176-178 CD denotes 15
T16874 179-185 NN denotes medium
T16876 185-186 . denotes .
T16877 186-489 sentence denotes For preparation of apoptotic target cells, primary thymocytes were harvested from the thymus of 4- to 8-week-old C57BL/6J mice, stained with TAMRA for 15 min, and apoptosis was induced either by treating cells with 5 μM staurosporine in medium for 4 h at 37°C or by culturing cells in medium overnight.
T16878 187-190 IN denotes For
T16880 191-202 NN denotes preparation
T16881 203-205 IN denotes of
T16882 206-215 JJ denotes apoptotic
T16884 216-222 NN denotes target
T16883 223-228 NNS denotes cells
T16885 228-230 , denotes ,
T16886 230-237 JJ denotes primary
T16887 238-248 NNS denotes thymocytes
T16888 249-253 VBD denotes were
T16879 254-263 VBN denotes harvested
T16889 264-268 IN denotes from
T16890 269-272 DT denotes the
T16891 273-279 NN denotes thymus
T16892 280-282 IN denotes of
T16893 283-284 CD denotes 4
T16895 284-285 HYPH denotes -
T16896 286-288 IN denotes to
T16894 289-290 CD denotes 8
T16898 290-291 HYPH denotes -
T16897 291-295 NN denotes week
T16900 295-296 HYPH denotes -
T16899 296-299 JJ denotes old
T16902 300-305 NN denotes C57BL
T16904 305-306 HYPH denotes /
T16903 306-308 NN denotes 6J
T16901 309-313 NNS denotes mice
T16905 313-315 , denotes ,
T16906 315-322 VBN denotes stained
T16907 323-327 IN denotes with
T16908 328-333 NN denotes TAMRA
T16909 334-337 IN denotes for
T16910 338-340 CD denotes 15
T16911 341-344 NNS denotes min
T16912 344-346 , denotes ,
T16913 346-349 CC denotes and
T16914 350-359 NN denotes apoptosis
T16916 360-363 VBD denotes was
T16915 364-371 VBN denotes induced
T16917 372-378 CC denotes either
T16918 379-381 IN denotes by
T16919 382-390 VBG denotes treating
T16920 391-396 NNS denotes cells
T16921 397-401 IN denotes with
T16922 402-403 CD denotes 5
T16923 404-406 NN denotes μM
T16924 407-420 NN denotes staurosporine
T16925 421-423 IN denotes in
T16926 424-430 NN denotes medium
T16927 431-434 IN denotes for
T16928 435-436 CD denotes 4
T16929 437-438 NNS denotes h
T16930 439-441 IN denotes at
T16931 442-444 CD denotes 37
T16932 444-446 NN denotes °C
T16933 447-449 CC denotes or
T16934 450-452 IN denotes by
T16935 453-462 VBG denotes culturing
T16936 463-468 NNS denotes cells
T16937 469-471 IN denotes in
T16938 472-478 NN denotes medium
T16939 479-488 RB denotes overnight
T16940 488-489 . denotes .
T16941 489-592 sentence denotes The efficacy of apoptosis induction was compared in thymic target cells and controls by FACS analysis.
T16942 490-493 DT denotes The
T16943 494-502 NN denotes efficacy
T16945 503-505 IN denotes of
T16946 506-515 NN denotes apoptosis
T16947 516-525 NN denotes induction
T16948 526-529 VBD denotes was
T16944 530-538 VBN denotes compared
T16949 539-541 IN denotes in
T16950 542-548 JJ denotes thymic
T16952 549-555 NN denotes target
T16951 556-561 NNS denotes cells
T16953 562-565 CC denotes and
T16954 566-574 NNS denotes controls
T16955 575-577 IN denotes by
T16956 578-582 NN denotes FACS
T16957 583-591 NN denotes analysis
T16958 591-592 . denotes .
T16959 592-806 sentence denotes On average, 60% of the cells of the resulting population were apoptotic, with exposed PS on their surface, and less than 5% of the cells were necrotic, as confirmed by FITC-annexin V and propidium iodide staining.
T16960 593-595 IN denotes On
T16962 596-603 JJ denotes average
T16963 603-605 , denotes ,
T16964 605-607 CD denotes 60
T16965 607-608 NN denotes %
T16966 609-611 IN denotes of
T16967 612-615 DT denotes the
T16968 616-621 NNS denotes cells
T16969 622-624 IN denotes of
T16970 625-628 DT denotes the
T16972 629-638 VBG denotes resulting
T16971 639-649 NN denotes population
T16961 650-654 VBD denotes were
T16973 655-664 JJ denotes apoptotic
T16974 664-666 , denotes ,
T16975 666-670 IN denotes with
T16976 671-678 VBN denotes exposed
T16977 679-681 NN denotes PS
T16978 682-684 IN denotes on
T16979 685-690 PRP$ denotes their
T16980 691-698 NN denotes surface
T16981 698-700 , denotes ,
T16982 700-703 CC denotes and
T16983 704-708 JJR denotes less
T16985 709-713 IN denotes than
T16984 714-715 CD denotes 5
T16986 715-716 NN denotes %
T16988 717-719 IN denotes of
T16989 720-723 DT denotes the
T16990 724-729 NNS denotes cells
T16987 730-734 VBD denotes were
T16991 735-743 JJ denotes necrotic
T16992 743-745 , denotes ,
T16993 745-747 IN denotes as
T16994 748-757 VBN denotes confirmed
T16995 758-760 IN denotes by
T16996 761-765 NN denotes FITC
T16998 765-766 HYPH denotes -
T16997 766-773 NN denotes annexin
T16999 774-775 CD denotes V
T17000 776-779 CC denotes and
T17001 780-789 NN denotes propidium
T17002 790-796 NN denotes iodide
T17003 797-805 NN denotes staining
T17004 805-806 . denotes .
T17005 806-915 sentence denotes The apoptotic thymocytes obtained were washed with PBS and added to the prepared FLDM cultures (ratio 10:1).
T17006 807-810 DT denotes The
T17008 811-820 JJ denotes apoptotic
T17007 821-831 NNS denotes thymocytes
T17010 832-840 VBN denotes obtained
T17011 841-845 VBD denotes were
T17009 846-852 VBN denotes washed
T17012 853-857 IN denotes with
T17013 858-861 NN denotes PBS
T17014 862-865 CC denotes and
T17015 866-871 VBN denotes added
T17016 872-874 IN denotes to
T17017 875-878 DT denotes the
T17019 879-887 VBN denotes prepared
T17020 888-892 NN denotes FLDM
T17018 893-901 NNS denotes cultures
T17021 902-903 -LRB- denotes (
T17023 903-908 NN denotes ratio
T17022 909-911 CD denotes 10
T17024 911-912 SYM denotes :
T17025 912-913 CD denotes 1
T17026 913-914 -RRB- denotes )
T17027 914-915 . denotes .
T17028 915-976 sentence denotes Phagocytosis was then allowed to proceed at 37°C and 5% CO2.
T17029 916-928 NN denotes Phagocytosis
T17031 929-932 VBD denotes was
T17032 933-937 RB denotes then
T17030 938-945 VBN denotes allowed
T17033 946-948 TO denotes to
T17034 949-956 VB denotes proceed
T17035 957-959 IN denotes at
T17036 960-962 CD denotes 37
T17037 962-964 NN denotes °C
T17038 965-968 CC denotes and
T17039 969-970 CD denotes 5
T17040 970-971 NN denotes %
T17041 972-975 NN denotes CO2
T17042 975-976 . denotes .
T17043 976-1143 sentence denotes After the indicated time periods, the uptake of apoptotic cells by FLDMs was stopped by intensive washing of co-cultures with cold PBS to remove unphagocytosed cells.
T17044 977-982 IN denotes After
T17046 983-986 DT denotes the
T17048 987-996 VBN denotes indicated
T17049 997-1001 NN denotes time
T17047 1002-1009 NNS denotes periods
T17050 1009-1011 , denotes ,
T17051 1011-1014 DT denotes the
T17052 1015-1021 NN denotes uptake
T17053 1022-1024 IN denotes of
T17054 1025-1034 JJ denotes apoptotic
T17055 1035-1040 NNS denotes cells
T17056 1041-1043 IN denotes by
T17057 1044-1049 NNS denotes FLDMs
T17058 1050-1053 VBD denotes was
T17045 1054-1061 VBN denotes stopped
T17059 1062-1064 IN denotes by
T17060 1065-1074 JJ denotes intensive
T17061 1075-1082 NN denotes washing
T17062 1083-1085 IN denotes of
T17063 1086-1097 NNS denotes co-cultures
T17064 1098-1102 IN denotes with
T17065 1103-1107 JJ denotes cold
T17066 1108-1111 NN denotes PBS
T17067 1112-1114 TO denotes to
T17068 1115-1121 VB denotes remove
T17069 1122-1136 JJ denotes unphagocytosed
T17070 1137-1142 NNS denotes cells
T17071 1142-1143 . denotes .
T17072 1143-1260 sentence denotes To measure phagocytosis of apoptotic thymocytes, macrophages were further processed for immunofluorescence analysis.
T17073 1144-1146 TO denotes To
T17074 1147-1154 VB denotes measure
T17076 1155-1167 NN denotes phagocytosis
T17077 1168-1170 IN denotes of
T17078 1171-1180 JJ denotes apoptotic
T17079 1181-1191 NNS denotes thymocytes
T17080 1191-1193 , denotes ,
T17081 1193-1204 NNS denotes macrophages
T17082 1205-1209 VBD denotes were
T17083 1210-1217 RB denotes further
T17075 1218-1227 VBN denotes processed
T17084 1228-1231 IN denotes for
T17085 1232-1250 NN denotes immunofluorescence
T17086 1251-1259 NN denotes analysis
T17087 1259-1260 . denotes .
T17088 1260-1467 sentence denotes Cells were fixed in 4% paraformaldehyde, blocked in 0.5% BSA/PBS and stained with an anti-F4/80 antibody (Serotec) followed by a secondary antibody coupled to Alexa 488 (Molecular Probes Inc., Eugene, USA).
T17089 1261-1266 NNS denotes Cells
T17091 1267-1271 VBD denotes were
T17090 1272-1277 VBN denotes fixed
T17092 1278-1280 IN denotes in
T17093 1281-1282 CD denotes 4
T17094 1282-1283 NN denotes %
T17095 1284-1300 NN denotes paraformaldehyde
T17096 1300-1302 , denotes ,
T17097 1302-1309 VBD denotes blocked
T17098 1310-1312 IN denotes in
T17099 1313-1316 CD denotes 0.5
T17100 1316-1317 NN denotes %
T17102 1318-1321 NN denotes BSA
T17103 1321-1322 HYPH denotes /
T17101 1322-1325 NN denotes PBS
T17104 1326-1329 CC denotes and
T17105 1330-1337 VBD denotes stained
T17106 1338-1342 IN denotes with
T17107 1343-1345 DT denotes an
T17109 1346-1353 JJ denotes anti-F4
T17111 1353-1354 HYPH denotes /
T17110 1354-1356 CD denotes 80
T17108 1357-1365 NN denotes antibody
T17112 1366-1367 -LRB- denotes (
T17113 1367-1374 NNP denotes Serotec
T17114 1374-1375 -RRB- denotes )
T17115 1376-1384 VBN denotes followed
T17116 1385-1387 IN denotes by
T17117 1388-1389 DT denotes a
T17119 1390-1399 JJ denotes secondary
T17118 1400-1408 NN denotes antibody
T17120 1409-1416 VBN denotes coupled
T17121 1417-1419 IN denotes to
T17122 1420-1425 NNP denotes Alexa
T17123 1426-1429 CD denotes 488
T17124 1430-1431 -LRB- denotes (
T17126 1431-1440 NNP denotes Molecular
T17127 1441-1447 NNP denotes Probes
T17125 1448-1452 NNP denotes Inc.
T17128 1452-1454 , denotes ,
T17129 1454-1460 NNP denotes Eugene
T17130 1460-1462 , denotes ,
T17131 1462-1465 NNP denotes USA
T17132 1465-1466 -RRB- denotes )
T17133 1466-1467 . denotes .
T17134 1467-1569 sentence denotes Coverslips were mounted on slides and engulfed thymocytes were enumerated by fluorescence microscopy.
T17135 1468-1478 NNS denotes Coverslips
T17137 1479-1483 VBD denotes were
T17136 1484-1491 VBN denotes mounted
T17138 1492-1494 IN denotes on
T17139 1495-1501 NNS denotes slides
T17140 1502-1505 CC denotes and
T17141 1506-1514 VBN denotes engulfed
T17142 1515-1525 NNS denotes thymocytes
T17144 1526-1530 VBD denotes were
T17143 1531-1541 VBN denotes enumerated
T17145 1542-1544 IN denotes by
T17146 1545-1557 NN denotes fluorescence
T17147 1558-1568 NN denotes microscopy
T17148 1568-1569 . denotes .
T17149 1569-1734 sentence denotes The percentage of phagocytosis was calculated by counting at least 300 macrophages and determining the number of macrophages that had engulfed apoptotic thymocytes.
T17150 1570-1573 DT denotes The
T17151 1574-1584 NN denotes percentage
T17153 1585-1587 IN denotes of
T17154 1588-1600 NN denotes phagocytosis
T17155 1601-1604 VBD denotes was
T17152 1605-1615 VBN denotes calculated
T17156 1616-1618 IN denotes by
T17157 1619-1627 VBG denotes counting
T17158 1628-1630 RB denotes at
T17160 1631-1636 RBS denotes least
T17159 1637-1640 CD denotes 300
T17161 1641-1652 NNS denotes macrophages
T17162 1653-1656 CC denotes and
T17163 1657-1668 VBG denotes determining
T17164 1669-1672 DT denotes the
T17165 1673-1679 NN denotes number
T17166 1680-1682 IN denotes of
T17167 1683-1694 NNS denotes macrophages
T17168 1695-1699 WDT denotes that
T17170 1700-1703 VBD denotes had
T17169 1704-1712 VBN denotes engulfed
T17171 1713-1722 JJ denotes apoptotic
T17172 1723-1733 NNS denotes thymocytes
T17173 1733-1734 . denotes .
T17174 1734-1993 sentence denotes The phagocytotic index was calculated according to the following formula: phagocytotic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.
T17175 1735-1738 DT denotes The
T17177 1739-1751 JJ denotes phagocytotic
T17176 1752-1757 NN denotes index
T17179 1758-1761 VBD denotes was
T17178 1762-1772 VBN denotes calculated
T17181 1773-1782 VBG denotes according
T17182 1783-1785 IN denotes to
T17183 1786-1789 DT denotes the
T17185 1790-1799 VBG denotes following
T17184 1800-1807 NN denotes formula
T17186 1807-1809 : denotes :
T17187 1809-1821 JJ denotes phagocytotic
T17188 1822-1827 NN denotes index
T17189 1828-1829 SYM denotes =
T17190 1830-1831 -LRB- denotes (
T17191 1831-1836 JJ denotes total
T17180 1837-1843 NN denotes number
T17192 1844-1846 IN denotes of
T17193 1847-1855 VBN denotes engulfed
T17194 1856-1861 NNS denotes cells
T17195 1861-1862 SYM denotes /
T17197 1862-1867 JJ denotes total
T17196 1868-1874 NN denotes number
T17198 1875-1877 IN denotes of
T17199 1878-1885 VBN denotes counted
T17200 1886-1897 NNS denotes macrophages
T17201 1897-1898 -RRB- denotes )
T17202 1899-1900 SYM denotes ×
T17204 1901-1902 -LRB- denotes (
T17203 1902-1908 NN denotes number
T17205 1909-1911 IN denotes of
T17206 1912-1923 NNS denotes macrophages
T17207 1924-1934 VBG denotes containing
T17208 1935-1943 VBN denotes engulfed
T17209 1944-1949 NNS denotes cells
T17210 1949-1950 SYM denotes /
T17212 1950-1955 JJ denotes total
T17211 1956-1962 NN denotes number
T17213 1963-1965 IN denotes of
T17214 1966-1973 VBN denotes counted
T17215 1974-1985 NNS denotes macrophages
T17216 1985-1986 -RRB- denotes )
T17217 1987-1988 SYM denotes ×
T17218 1989-1992 CD denotes 100
T17219 1992-1993 . denotes .
T17220 1993-2131 sentence denotes The experiments were performed at least three times, each time in triplicate, and the counting was done by three different investigators.
T17221 1994-1997 DT denotes The
T17222 1998-2009 NNS denotes experiments
T17224 2010-2014 VBD denotes were
T17223 2015-2024 VBN denotes performed
T17225 2025-2027 RB denotes at
T17227 2028-2033 RBS denotes least
T17226 2034-2039 CD denotes three
T17228 2040-2045 NNS denotes times
T17229 2045-2047 , denotes ,
T17230 2047-2051 DT denotes each
T17231 2052-2056 NN denotes time
T17232 2057-2059 IN denotes in
T17233 2060-2070 NN denotes triplicate
T17234 2070-2072 , denotes ,
T17235 2072-2075 CC denotes and
T17236 2076-2079 DT denotes the
T17237 2080-2088 NN denotes counting
T17239 2089-2092 VBD denotes was
T17238 2093-2097 VBN denotes done
T17240 2098-2100 IN denotes by
T17241 2101-2106 CD denotes three
T17243 2107-2116 JJ denotes different
T17242 2117-2130 NNS denotes investigators
T17244 2130-2131 . denotes .
R10276 T16840 T16841 compound Macrophage,assays
R10277 T16842 T16841 compound phagocytosis,assays
R10278 T16844 T16845 prep For,plated
R10279 T16846 T16844 pobj preparation,For
R10280 T16847 T16846 prep of,preparation
R10281 T16848 T16849 compound monolayer,cultures
R10282 T16849 T16847 pobj cultures,of
R10283 T16850 T16849 prep of,cultures
R10284 T16851 T16850 pobj macrophages,of
R10285 T16852 T16845 punct ", ",plated
R10286 T16853 T16845 nsubjpass FLDMs,plated
R10287 T16854 T16845 auxpass were,plated
R10288 T16855 T16845 prep on,plated
R10289 T16856 T16857 compound glass,coverslips
R10290 T16857 T16855 pobj coverslips,on
R10291 T16858 T16845 prep in,plated
R10292 T16859 T16860 nummod 24,plates
R10293 T16860 T16858 pobj plates,in
R10294 T16861 T16860 compound well,plates
R10295 T16862 T16863 punct (,cells
R10296 T16863 T16858 parataxis cells,in
R10297 T16864 T16865 quantmod 2,105
R10298 T16865 T16863 nummod 105,cells
R10299 T16866 T16865 punct ×,105
R10300 T16867 T16863 prep per,cells
R10301 T16868 T16867 pobj well,per
R10302 T16869 T16863 punct ),cells
R10303 T16870 T16845 prep in,plated
R10304 T16871 T16872 nmod X,Vivo
R10305 T16872 T16874 nmod Vivo,medium
R10306 T16873 T16872 punct -,Vivo
R10307 T16874 T16870 pobj medium,in
R10308 T16875 T16872 nummod 15,Vivo
R10309 T16876 T16845 punct .,plated
R10310 T16878 T16879 prep For,harvested
R10311 T16880 T16878 pobj preparation,For
R10312 T16881 T16880 prep of,preparation
R10313 T16882 T16883 amod apoptotic,cells
R10314 T16883 T16881 pobj cells,of
R10315 T16884 T16883 compound target,cells
R10316 T16885 T16879 punct ", ",harvested
R10317 T16886 T16887 amod primary,thymocytes
R10318 T16887 T16879 nsubjpass thymocytes,harvested
R10319 T16888 T16879 auxpass were,harvested
R10320 T16889 T16879 prep from,harvested
R10321 T16890 T16891 det the,thymus
R10322 T16891 T16889 pobj thymus,from
R10323 T16892 T16891 prep of,thymus
R10324 T16893 T16894 quantmod 4,8
R10325 T16894 T16897 nummod 8,week
R10326 T16895 T16894 punct -,8
R10327 T16896 T16894 quantmod to,8
R10328 T16897 T16899 npadvmod week,old
R10329 T16898 T16897 punct -,week
R10330 T16899 T16901 amod old,mice
R10331 T16900 T16899 punct -,old
R10332 T16901 T16892 pobj mice,of
R10333 T16902 T16903 compound C57BL,6J
R10334 T16903 T16901 compound 6J,mice
R10335 T16904 T16903 punct /,6J
R10336 T16905 T16879 punct ", ",harvested
R10337 T16906 T16879 dep stained,harvested
R10338 T16907 T16906 prep with,stained
R10339 T16908 T16907 pobj TAMRA,with
R10340 T16909 T16906 prep for,stained
R10341 T16910 T16911 nummod 15,min
R10342 T16911 T16909 pobj min,for
R10343 T16912 T16879 punct ", ",harvested
R10344 T16913 T16879 cc and,harvested
R10345 T16914 T16915 nsubjpass apoptosis,induced
R10346 T16915 T16879 conj induced,harvested
R10347 T16916 T16915 auxpass was,induced
R10348 T16917 T16918 preconj either,by
R10349 T16918 T16915 prep by,induced
R10350 T16919 T16918 pcomp treating,by
R10351 T16920 T16919 dobj cells,treating
R10352 T16921 T16919 prep with,treating
R10353 T16922 T16923 nummod 5,μM
R10354 T16923 T16924 compound μM,staurosporine
R10355 T16924 T16921 pobj staurosporine,with
R10356 T16925 T16919 prep in,treating
R10357 T16926 T16925 pobj medium,in
R10358 T16927 T16919 prep for,treating
R10359 T16928 T16929 nummod 4,h
R10360 T16929 T16927 pobj h,for
R10361 T16930 T16919 prep at,treating
R10362 T16931 T16932 nummod 37,°C
R10363 T16932 T16930 pobj °C,at
R10364 T16933 T16918 cc or,by
R10365 T16934 T16918 conj by,by
R10366 T16935 T16934 pcomp culturing,by
R10367 T16936 T16935 dobj cells,culturing
R10368 T16937 T16935 prep in,culturing
R10369 T16938 T16937 pobj medium,in
R10370 T16939 T16935 advmod overnight,culturing
R10371 T16940 T16879 punct .,harvested
R10372 T16942 T16943 det The,efficacy
R10373 T16943 T16944 nsubjpass efficacy,compared
R10374 T16945 T16943 prep of,efficacy
R10375 T16946 T16947 compound apoptosis,induction
R10376 T16947 T16945 pobj induction,of
R10377 T16948 T16944 auxpass was,compared
R10378 T16949 T16944 prep in,compared
R10379 T16950 T16951 amod thymic,cells
R10380 T16951 T16949 pobj cells,in
R10381 T16952 T16951 compound target,cells
R10382 T16953 T16951 cc and,cells
R10383 T16954 T16951 conj controls,cells
R10384 T16955 T16944 prep by,compared
R10385 T16956 T16957 compound FACS,analysis
R10386 T16957 T16955 pobj analysis,by
R10387 T16958 T16944 punct .,compared
R10388 T16960 T16961 prep On,were
R10389 T16962 T16960 amod average,On
R10390 T16963 T16961 punct ", ",were
R10391 T16964 T16965 nummod 60,%
R10392 T16965 T16961 nsubj %,were
R10393 T16966 T16965 prep of,%
R10394 T16967 T16968 det the,cells
R10395 T16968 T16966 pobj cells,of
R10396 T16969 T16965 prep of,%
R10397 T16970 T16971 det the,population
R10398 T16971 T16969 pobj population,of
R10399 T16972 T16971 amod resulting,population
R10400 T16973 T16961 acomp apoptotic,were
R10401 T16974 T16961 punct ", ",were
R10402 T16975 T16961 prep with,were
R10403 T16976 T16977 amod exposed,PS
R10404 T16977 T16975 pobj PS,with
R10405 T16978 T16977 prep on,PS
R10406 T16979 T16980 poss their,surface
R10407 T16980 T16978 pobj surface,on
R10408 T16981 T16961 punct ", ",were
R10409 T16982 T16961 cc and,were
R10410 T16983 T16984 amod less,5
R10411 T16984 T16986 nummod 5,%
R10412 T16985 T16984 quantmod than,5
R10413 T16986 T16987 nsubj %,were
R10414 T16987 T16961 conj were,were
R10415 T16988 T16986 prep of,%
R10416 T16989 T16990 det the,cells
R10417 T16990 T16988 pobj cells,of
R10418 T16991 T16987 acomp necrotic,were
R10419 T16992 T16987 punct ", ",were
R10420 T16993 T16994 mark as,confirmed
R10421 T16994 T16987 advcl confirmed,were
R10422 T16995 T16994 prep by,confirmed
R10423 T16996 T16997 compound FITC,annexin
R10424 T16997 T16995 pobj annexin,by
R10425 T16998 T16997 punct -,annexin
R10426 T16999 T16997 nummod V,annexin
R10427 T17000 T16997 cc and,annexin
R10428 T17001 T17002 compound propidium,iodide
R10429 T17002 T17003 compound iodide,staining
R10430 T17003 T16997 conj staining,annexin
R10431 T17004 T16987 punct .,were
R10432 T17006 T17007 det The,thymocytes
R10433 T17007 T17009 nsubjpass thymocytes,washed
R10434 T17008 T17007 amod apoptotic,thymocytes
R10435 T17010 T17007 acl obtained,thymocytes
R10436 T17011 T17009 auxpass were,washed
R10437 T17012 T17009 prep with,washed
R10438 T17013 T17012 pobj PBS,with
R10439 T17014 T17009 cc and,washed
R10440 T17015 T17009 conj added,washed
R10441 T17016 T17015 prep to,added
R10442 T17017 T17018 det the,cultures
R10443 T17018 T17016 pobj cultures,to
R10444 T17019 T17018 amod prepared,cultures
R10445 T17020 T17018 compound FLDM,cultures
R10446 T17021 T17022 punct (,10
R10447 T17022 T17015 parataxis 10,added
R10448 T17023 T17022 dep ratio,10
R10449 T17024 T17025 punct :,1
R10450 T17025 T17022 prep 1,10
R10451 T17026 T17022 punct ),10
R10452 T17027 T17009 punct .,washed
R10453 T17029 T17030 nsubjpass Phagocytosis,allowed
R10454 T17031 T17030 auxpass was,allowed
R10455 T17032 T17030 advmod then,allowed
R10456 T17033 T17034 aux to,proceed
R10457 T17034 T17030 xcomp proceed,allowed
R10458 T17035 T17034 prep at,proceed
R10459 T17036 T17037 nummod 37,°C
R10460 T17037 T17035 pobj °C,at
R10461 T17038 T17037 cc and,°C
R10462 T17039 T17040 nummod 5,%
R10463 T17040 T17041 compound %,CO2
R10464 T17041 T17037 conj CO2,°C
R10465 T17042 T17030 punct .,allowed
R10466 T17044 T17045 prep After,stopped
R10467 T17046 T17047 det the,periods
R10468 T17047 T17044 pobj periods,After
R10469 T17048 T17047 amod indicated,periods
R10470 T17049 T17047 compound time,periods
R10471 T17050 T17045 punct ", ",stopped
R10472 T17051 T17052 det the,uptake
R10473 T17052 T17045 nsubjpass uptake,stopped
R10474 T17053 T17052 prep of,uptake
R10475 T17054 T17055 amod apoptotic,cells
R10476 T17055 T17053 pobj cells,of
R10477 T17056 T17052 prep by,uptake
R10478 T17057 T17056 pobj FLDMs,by
R10479 T17058 T17045 auxpass was,stopped
R10480 T17059 T17045 prep by,stopped
R10481 T17060 T17061 amod intensive,washing
R10482 T17061 T17059 pobj washing,by
R10483 T17062 T17061 prep of,washing
R10484 T17063 T17062 pobj co-cultures,of
R10485 T17064 T17061 prep with,washing
R10486 T17065 T17066 amod cold,PBS
R10487 T17066 T17064 pobj PBS,with
R10488 T17067 T17068 aux to,remove
R10489 T17068 T17045 advcl remove,stopped
R10490 T17069 T17070 amod unphagocytosed,cells
R10491 T17070 T17068 dobj cells,remove
R10492 T17071 T17045 punct .,stopped
R10493 T17073 T17074 aux To,measure
R10494 T17074 T17075 advcl measure,processed
R10495 T17076 T17074 dobj phagocytosis,measure
R10496 T17077 T17076 prep of,phagocytosis
R10497 T17078 T17079 amod apoptotic,thymocytes
R10498 T17079 T17077 pobj thymocytes,of
R10499 T17080 T17075 punct ", ",processed
R10500 T17081 T17075 nsubjpass macrophages,processed
R10501 T17082 T17075 auxpass were,processed
R10502 T17083 T17075 advmod further,processed
R10503 T17084 T17075 prep for,processed
R10504 T17085 T17086 compound immunofluorescence,analysis
R10505 T17086 T17084 pobj analysis,for
R10506 T17087 T17075 punct .,processed
R10507 T17089 T17090 nsubjpass Cells,fixed
R10508 T17091 T17090 auxpass were,fixed
R10509 T17092 T17090 prep in,fixed
R10510 T17093 T17094 nummod 4,%
R10511 T17094 T17095 compound %,paraformaldehyde
R10512 T17095 T17092 pobj paraformaldehyde,in
R10513 T17096 T17090 punct ", ",fixed
R10514 T17097 T17090 conj blocked,fixed
R10515 T17098 T17097 prep in,blocked
R10516 T17099 T17100 nummod 0.5,%
R10517 T17100 T17101 compound %,PBS
R10518 T17101 T17098 pobj PBS,in
R10519 T17102 T17101 compound BSA,PBS
R10520 T17103 T17101 punct /,PBS
R10521 T17104 T17097 cc and,blocked
R10522 T17105 T17097 conj stained,blocked
R10523 T17106 T17105 prep with,stained
R10524 T17107 T17108 det an,antibody
R10525 T17108 T17106 pobj antibody,with
R10526 T17109 T17110 amod anti-F4,80
R10527 T17110 T17108 nummod 80,antibody
R10528 T17111 T17110 punct /,80
R10529 T17112 T17113 punct (,Serotec
R10530 T17113 T17108 parataxis Serotec,antibody
R10531 T17114 T17113 punct ),Serotec
R10532 T17115 T17108 acl followed,antibody
R10533 T17116 T17115 agent by,followed
R10534 T17117 T17118 det a,antibody
R10535 T17118 T17116 pobj antibody,by
R10536 T17119 T17118 amod secondary,antibody
R10537 T17120 T17118 acl coupled,antibody
R10538 T17121 T17120 prep to,coupled
R10539 T17122 T17121 pobj Alexa,to
R10540 T17123 T17122 nummod 488,Alexa
R10541 T17124 T17125 punct (,Inc.
R10542 T17125 T17122 parataxis Inc.,Alexa
R10543 T17126 T17125 compound Molecular,Inc.
R10544 T17127 T17125 compound Probes,Inc.
R10545 T17128 T17125 punct ", ",Inc.
R10546 T17129 T17125 npadvmod Eugene,Inc.
R10547 T17130 T17125 punct ", ",Inc.
R10548 T17131 T17125 npadvmod USA,Inc.
R10549 T17132 T17125 punct ),Inc.
R10550 T17133 T17090 punct .,fixed
R10551 T17135 T17136 nsubjpass Coverslips,mounted
R10552 T17137 T17136 auxpass were,mounted
R10553 T17138 T17136 prep on,mounted
R10554 T17139 T17138 pobj slides,on
R10555 T17140 T17136 cc and,mounted
R10556 T17141 T17142 amod engulfed,thymocytes
R10557 T17142 T17143 nsubjpass thymocytes,enumerated
R10558 T17143 T17136 conj enumerated,mounted
R10559 T17144 T17143 auxpass were,enumerated
R10560 T17145 T17143 prep by,enumerated
R10561 T17146 T17147 compound fluorescence,microscopy
R10562 T17147 T17145 pobj microscopy,by
R10563 T17148 T17143 punct .,enumerated
R10564 T17150 T17151 det The,percentage
R10565 T17151 T17152 nsubjpass percentage,calculated
R10566 T17153 T17151 prep of,percentage
R10567 T17154 T17153 pobj phagocytosis,of
R10568 T17155 T17152 auxpass was,calculated
R10569 T17156 T17152 prep by,calculated
R10570 T17157 T17156 pcomp counting,by
R10571 T17158 T17159 advmod at,300
R10572 T17159 T17161 nummod 300,macrophages
R10573 T17160 T17159 advmod least,300
R10574 T17161 T17157 dobj macrophages,counting
R10575 T17162 T17157 cc and,counting
R10576 T17163 T17157 conj determining,counting
R10577 T17164 T17165 det the,number
R10578 T17165 T17163 dobj number,determining
R10579 T17166 T17165 prep of,number
R10580 T17167 T17166 pobj macrophages,of
R10581 T17168 T17169 dep that,engulfed
R10582 T17169 T17167 relcl engulfed,macrophages
R10583 T17170 T17169 aux had,engulfed
R10584 T17171 T17172 amod apoptotic,thymocytes
R10585 T17172 T17169 dobj thymocytes,engulfed
R10586 T17173 T17152 punct .,calculated
R10587 T17175 T17176 det The,index
R10588 T17176 T17178 nsubjpass index,calculated
R10589 T17177 T17176 amod phagocytotic,index
R10590 T17178 T17180 ccomp calculated,number
R10591 T17179 T17178 auxpass was,calculated
R10592 T17181 T17178 prep according,calculated
R10593 T17182 T17181 prep to,according
R10594 T17183 T17184 det the,formula
R10595 T17184 T17182 pobj formula,to
R10596 T17185 T17184 amod following,formula
R10597 T17186 T17180 punct : ,number
R10598 T17187 T17188 amod phagocytotic,index
R10599 T17188 T17180 nsubj index,number
R10600 T17189 T17180 punct =,number
R10601 T17190 T17180 punct (,number
R10602 T17191 T17180 amod total,number
R10603 T17192 T17180 prep of,number
R10604 T17193 T17194 amod engulfed,cells
R10605 T17194 T17192 pobj cells,of
R10606 T17195 T17196 punct /,number
R10607 T17196 T17180 prep number,number
R10608 T17197 T17196 amod total,number
R10609 T17198 T17196 prep of,number
R10610 T17199 T17200 amod counted,macrophages
R10611 T17200 T17198 pobj macrophages,of
R10612 T17201 T17180 punct ),number
R10613 T17202 T17203 punct ×,number
R10614 T17203 T17180 prep number,number
R10615 T17204 T17203 punct (,number
R10616 T17205 T17203 prep of,number
R10617 T17206 T17205 pobj macrophages,of
R10618 T17207 T17206 acl containing,macrophages
R10619 T17208 T17209 amod engulfed,cells
R10620 T17209 T17207 dobj cells,containing
R10621 T17210 T17211 punct /,number
R10622 T17211 T17203 prep number,number
R10623 T17212 T17211 amod total,number
R10624 T17213 T17211 prep of,number
R10625 T17214 T17215 amod counted,macrophages
R10626 T17215 T17213 pobj macrophages,of
R10627 T17216 T17203 punct ),number
R10628 T17217 T17218 punct ×,100
R10629 T17218 T17180 prep 100,number
R10630 T17219 T17180 punct .,number
R10631 T17221 T17222 det The,experiments
R10632 T17222 T17223 nsubjpass experiments,performed
R10633 T17224 T17223 auxpass were,performed
R10634 T17225 T17226 advmod at,three
R10635 T17226 T17228 nummod three,times
R10636 T17227 T17226 advmod least,three
R10637 T17228 T17223 npadvmod times,performed
R10638 T17229 T17223 punct ", ",performed
R10639 T17230 T17231 det each,time
R10640 T17231 T17223 npadvmod time,performed
R10641 T17232 T17231 prep in,time
R10642 T17233 T17232 pobj triplicate,in
R10643 T17234 T17223 punct ", ",performed
R10644 T17235 T17223 cc and,performed
R10645 T17236 T17237 det the,counting
R10646 T17237 T17238 nsubjpass counting,done
R10647 T17238 T17223 conj done,performed
R10648 T17239 T17238 auxpass was,done
R10649 T17240 T17238 agent by,done
R10650 T17241 T17242 nummod three,investigators
R10651 T17242 T17240 pobj investigators,by
R10652 T17243 T17242 amod different,investigators
R10653 T17244 T17238 punct .,done