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PMC:549712 / 50445-51109 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T14739 0-5 NN denotes Ptdsr
T14741 6-10 NN denotes gene
T14742 10-11 HYPH denotes -
T14740 11-15 NN denotes trap
T14743 16-19 CC denotes and
T14744 20-30 NN denotes generation
T14745 31-33 IN denotes of
T14746 34-38 NN denotes germ
T14748 38-39 HYPH denotes -
T14747 39-43 NN denotes line
T14749 44-52 NNS denotes chimeras
T14750 52-247 sentence denotes An ES-cell line carrying a β-geo gene-trap vector in the Ptdsr locus was identified by searching the BayGenomics database (BayGenomics, San Francisco, USA; [48]) with the full-length Ptdsr cDNA.
T14751 53-55 DT denotes An
T14753 56-58 NN denotes ES
T14755 58-59 HYPH denotes -
T14754 59-63 NN denotes cell
T14752 64-68 NN denotes line
T14757 69-77 VBG denotes carrying
T14758 78-79 DT denotes a
T14760 80-81 NN denotes β
T14762 81-82 HYPH denotes -
T14761 82-85 NN denotes geo
T14763 86-90 NN denotes gene
T14765 90-91 HYPH denotes -
T14764 91-95 NN denotes trap
T14759 96-102 NN denotes vector
T14766 103-105 IN denotes in
T14767 106-109 DT denotes the
T14769 110-115 NN denotes Ptdsr
T14768 116-121 NN denotes locus
T14770 122-125 VBD denotes was
T14756 126-136 VBN denotes identified
T14771 137-139 IN denotes by
T14772 140-149 VBG denotes searching
T14773 150-153 DT denotes the
T14775 154-165 NNP denotes BayGenomics
T14774 166-174 NN denotes database
T14776 175-176 -LRB- denotes (
T14778 176-187 NNP denotes BayGenomics
T14779 187-189 , denotes ,
T14780 189-192 NNP denotes San
T14781 193-202 NNP denotes Francisco
T14782 202-204 , denotes ,
T14783 204-207 NNP denotes USA
T14784 207-208 : denotes ;
T14785 209-210 -LRB- denotes [
T14777 210-212 CD denotes 48
T14786 212-213 -RRB- denotes ]
T14787 213-214 -RRB- denotes )
T14788 215-219 IN denotes with
T14789 220-223 DT denotes the
T14791 224-228 JJ denotes full
T14793 228-229 HYPH denotes -
T14792 229-235 NN denotes length
T14794 236-241 NN denotes Ptdsr
T14790 242-246 NN denotes cDNA
T14795 246-247 . denotes .
T14796 247-362 sentence denotes A single ES-cell line was identified carrying the gene-trap in intron V, between exons V and VI of the Ptdsr gene.
T14797 248-249 DT denotes A
T14799 250-256 JJ denotes single
T14800 257-259 NN denotes ES
T14802 259-260 HYPH denotes -
T14801 260-264 NN denotes cell
T14798 265-269 NN denotes line
T14804 270-273 VBD denotes was
T14805 274-284 VBN denotes identified
T14803 285-293 VBG denotes carrying
T14806 294-297 DT denotes the
T14808 298-302 NN denotes gene
T14809 302-303 HYPH denotes -
T14807 303-307 NN denotes trap
T14810 308-310 IN denotes in
T14811 311-317 NN denotes intron
T14812 318-319 CD denotes V
T14813 319-321 , denotes ,
T14814 321-328 IN denotes between
T14815 329-334 NNS denotes exons
T14816 335-336 CD denotes V
T14817 337-340 CC denotes and
T14818 341-343 CD denotes VI
T14819 344-346 IN denotes of
T14820 347-350 DT denotes the
T14822 351-356 NN denotes Ptdsr
T14821 357-361 NN denotes gene
T14823 361-362 . denotes .
T14824 362-478 sentence denotes Chimeric mice were generated by microinjection into CB20 blastocysts and transfer to pseudopregnant foster mothers.
T14825 363-371 JJ denotes Chimeric
T14826 372-376 NNS denotes mice
T14828 377-381 VBD denotes were
T14827 382-391 VBN denotes generated
T14829 392-394 IN denotes by
T14830 395-409 NN denotes microinjection
T14831 410-414 IN denotes into
T14832 415-419 NN denotes CB20
T14833 420-431 NNS denotes blastocysts
T14834 432-435 CC denotes and
T14835 436-444 NN denotes transfer
T14836 445-447 IN denotes to
T14837 448-462 JJ denotes pseudopregnant
T14839 463-469 JJ denotes foster
T14838 470-477 NNS denotes mothers
T14840 477-478 . denotes .
T14841 478-531 sentence denotes Chimeric males were mated with 129P2/OlaHsd females.
T14842 479-487 JJ denotes Chimeric
T14843 488-493 NNS denotes males
T14845 494-498 VBD denotes were
T14844 499-504 VBN denotes mated
T14846 505-509 IN denotes with
T14847 510-515 NN denotes 129P2
T14849 515-516 HYPH denotes /
T14848 516-522 NN denotes OlaHsd
T14850 523-530 NNS denotes females
T14851 530-531 . denotes .
T14852 531-664 sentence denotes Germ-line transmission of the mutant gene-trap allele was verified by expression analysis using β-galactosidase staining and RT-PCR.
T14853 532-536 NN denotes Germ
T14855 536-537 HYPH denotes -
T14854 537-541 NN denotes line
T14856 542-554 NN denotes transmission
T14858 555-557 IN denotes of
T14859 558-561 DT denotes the
T14861 562-568 JJ denotes mutant
T14862 569-573 NN denotes gene
T14864 573-574 HYPH denotes -
T14863 574-578 NN denotes trap
T14860 579-585 NN denotes allele
T14865 586-589 VBD denotes was
T14857 590-598 VBN denotes verified
T14866 599-601 IN denotes by
T14867 602-612 NN denotes expression
T14868 613-621 NN denotes analysis
T14869 622-627 VBG denotes using
T14870 628-629 NN denotes β
T14872 629-630 HYPH denotes -
T14871 630-643 NN denotes galactosidase
T14873 644-652 NN denotes staining
T14874 653-656 CC denotes and
T14875 657-659 NN denotes RT
T14877 659-660 HYPH denotes -
T14876 660-663 NN denotes PCR
T14878 663-664 . denotes .
R8876 T14739 T14740 compound Ptdsr,trap
R8877 T14741 T14740 compound gene,trap
R8878 T14742 T14740 punct -,trap
R8879 T14743 T14740 cc and,trap
R8880 T14744 T14740 conj generation,trap
R8881 T14745 T14744 prep of,generation
R8882 T14746 T14747 compound germ,line
R8883 T14747 T14749 compound line,chimeras
R8884 T14748 T14747 punct -,line
R8885 T14749 T14745 pobj chimeras,of
R8886 T14751 T14752 det An,line
R8887 T14752 T14756 nsubjpass line,identified
R8888 T14753 T14754 compound ES,cell
R8889 T14754 T14752 compound cell,line
R8890 T14755 T14754 punct -,cell
R8891 T14757 T14752 acl carrying,line
R8892 T14758 T14759 det a,vector
R8893 T14759 T14757 dobj vector,carrying
R8894 T14760 T14761 compound β,geo
R8895 T14761 T14759 compound geo,vector
R8896 T14762 T14761 punct -,geo
R8897 T14763 T14764 compound gene,trap
R8898 T14764 T14759 compound trap,vector
R8899 T14765 T14764 punct -,trap
R8900 T14766 T14757 prep in,carrying
R8901 T14767 T14768 det the,locus
R8902 T14768 T14766 pobj locus,in
R8903 T14769 T14768 compound Ptdsr,locus
R8904 T14770 T14756 auxpass was,identified
R8905 T14771 T14756 prep by,identified
R8906 T14772 T14771 pcomp searching,by
R8907 T14773 T14774 det the,database
R8908 T14774 T14772 dobj database,searching
R8909 T14775 T14774 compound BayGenomics,database
R8910 T14776 T14777 punct (,48
R8911 T14777 T14774 parataxis 48,database
R8912 T14778 T14777 dep BayGenomics,48
R8913 T14779 T14778 punct ", ",BayGenomics
R8914 T14780 T14781 compound San,Francisco
R8915 T14781 T14778 npadvmod Francisco,BayGenomics
R8916 T14782 T14778 punct ", ",BayGenomics
R8917 T14783 T14778 npadvmod USA,BayGenomics
R8918 T14784 T14777 punct ;,48
R8919 T14785 T14777 punct [,48
R8920 T14786 T14777 punct ],48
R8921 T14787 T14777 punct ),48
R8922 T14788 T14772 prep with,searching
R8923 T14789 T14790 det the,cDNA
R8924 T14790 T14788 pobj cDNA,with
R8925 T14791 T14792 amod full,length
R8926 T14792 T14790 compound length,cDNA
R8927 T14793 T14792 punct -,length
R8928 T14794 T14790 compound Ptdsr,cDNA
R8929 T14795 T14756 punct .,identified
R8930 T14797 T14798 det A,line
R8931 T14798 T14803 nsubjpass line,carrying
R8932 T14799 T14798 amod single,line
R8933 T14800 T14801 compound ES,cell
R8934 T14801 T14798 compound cell,line
R8935 T14802 T14801 punct -,cell
R8936 T14804 T14803 auxpass was,carrying
R8937 T14805 T14803 aux identified,carrying
R8938 T14806 T14807 det the,trap
R8939 T14807 T14803 dobj trap,carrying
R8940 T14808 T14807 compound gene,trap
R8941 T14809 T14807 punct -,trap
R8942 T14810 T14803 prep in,carrying
R8943 T14811 T14810 pobj intron,in
R8944 T14812 T14811 nummod V,intron
R8945 T14813 T14811 punct ", ",intron
R8946 T14814 T14811 prep between,intron
R8947 T14815 T14816 nmod exons,V
R8948 T14816 T14814 pobj V,between
R8949 T14817 T14816 cc and,V
R8950 T14818 T14816 conj VI,V
R8951 T14819 T14816 prep of,V
R8952 T14820 T14821 det the,gene
R8953 T14821 T14819 pobj gene,of
R8954 T14822 T14821 compound Ptdsr,gene
R8955 T14823 T14803 punct .,carrying
R8956 T14825 T14826 amod Chimeric,mice
R8957 T14826 T14827 nsubjpass mice,generated
R8958 T14828 T14827 auxpass were,generated
R8959 T14829 T14827 prep by,generated
R8960 T14830 T14829 pobj microinjection,by
R8961 T14831 T14830 prep into,microinjection
R8962 T14832 T14833 compound CB20,blastocysts
R8963 T14833 T14831 pobj blastocysts,into
R8964 T14834 T14830 cc and,microinjection
R8965 T14835 T14830 conj transfer,microinjection
R8966 T14836 T14835 prep to,transfer
R8967 T14837 T14838 amod pseudopregnant,mothers
R8968 T14838 T14836 pobj mothers,to
R8969 T14839 T14838 amod foster,mothers
R8970 T14840 T14827 punct .,generated
R8971 T14842 T14843 amod Chimeric,males
R8972 T14843 T14844 nsubjpass males,mated
R8973 T14845 T14844 auxpass were,mated
R8974 T14846 T14844 prep with,mated
R8975 T14847 T14848 compound 129P2,OlaHsd
R8976 T14848 T14850 compound OlaHsd,females
R8977 T14849 T14848 punct /,OlaHsd
R8978 T14850 T14846 pobj females,with
R8979 T14851 T14844 punct .,mated
R8980 T14853 T14854 compound Germ,line
R8981 T14854 T14856 compound line,transmission
R8982 T14855 T14854 punct -,line
R8983 T14856 T14857 nsubjpass transmission,verified
R8984 T14858 T14856 prep of,transmission
R8985 T14859 T14860 det the,allele
R8986 T14860 T14858 pobj allele,of
R8987 T14861 T14860 amod mutant,allele
R8988 T14862 T14863 compound gene,trap
R8989 T14863 T14860 compound trap,allele
R8990 T14864 T14863 punct -,trap
R8991 T14865 T14857 auxpass was,verified
R8992 T14866 T14857 prep by,verified
R8993 T14867 T14868 compound expression,analysis
R8994 T14868 T14866 pobj analysis,by
R8995 T14869 T14857 advcl using,verified
R8996 T14870 T14871 compound β,galactosidase
R8997 T14871 T14873 compound galactosidase,staining
R8998 T14872 T14871 punct -,galactosidase
R8999 T14873 T14869 dobj staining,using
R9000 T14874 T14873 cc and,staining
R9001 T14875 T14876 compound RT,PCR
R9002 T14876 T14873 conj PCR,staining
R9003 T14877 T14876 punct -,PCR
R9004 T14878 T14857 punct .,verified