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PMC:549712 / 49011-60842 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T14458 23-35 NN denotes Construction
T14459 36-38 IN denotes of
T14460 39-42 DT denotes the
T14462 43-52 NN denotes targeting
T14461 53-59 NN denotes vector
T14463 60-63 CC denotes and
T14464 64-74 NN denotes generation
T14465 75-77 IN denotes of
T14466 78-83 NN denotes Ptdsr
T14468 83-84 HYPH denotes -
T14467 84-92 NN denotes knockout
T14470 93-96 CC denotes and
T14471 97-101 NN denotes gene
T14473 101-102 HYPH denotes -
T14472 102-106 NN denotes trap
T14469 107-111 NNS denotes mice
T14474 111-129 sentence denotes Targeting vector
T14475 113-122 NN denotes Targeting
T14476 123-129 NN denotes vector
T14477 129-352 sentence denotes A Ptdsr-containing bacterial artificial chromosome (BAC) clone (GenBank accession number AC091694; RP-23-316F3) was isolated by sequence homology from a C57BL/6J genomic BAC library (RP-23; BACPAC Resources, Oakland, USA).
T14478 130-131 DT denotes A
T14480 132-137 NN denotes Ptdsr
T14482 137-138 HYPH denotes -
T14481 138-148 VBG denotes containing
T14483 149-158 JJ denotes bacterial
T14485 159-169 JJ denotes artificial
T14484 170-180 NN denotes chromosome
T14486 181-182 -LRB- denotes (
T14487 182-185 NN denotes BAC
T14488 185-186 -RRB- denotes )
T14479 187-192 NN denotes clone
T14490 193-194 -LRB- denotes (
T14492 194-201 NNP denotes GenBank
T14494 202-211 NN denotes accession
T14495 212-218 NN denotes number
T14493 219-227 NN denotes AC091694
T14496 227-228 : denotes ;
T14491 229-231 NN denotes RP
T14497 231-232 HYPH denotes -
T14498 232-234 CD denotes 23
T14499 234-235 HYPH denotes -
T14500 235-240 CD denotes 316F3
T14501 240-241 -RRB- denotes )
T14502 242-245 VBD denotes was
T14489 246-254 VBN denotes isolated
T14503 255-257 IN denotes by
T14504 258-266 NN denotes sequence
T14505 267-275 NN denotes homology
T14506 276-280 IN denotes from
T14507 281-282 DT denotes a
T14509 283-288 NN denotes C57BL
T14511 288-289 HYPH denotes /
T14510 289-291 NN denotes 6J
T14512 292-299 JJ denotes genomic
T14513 300-303 NN denotes BAC
T14508 304-311 NN denotes library
T14514 312-313 -LRB- denotes (
T14516 313-315 NN denotes RP
T14517 315-316 HYPH denotes -
T14518 316-318 CD denotes 23
T14519 318-319 : denotes ;
T14520 320-326 NN denotes BACPAC
T14515 327-336 NNS denotes Resources
T14521 336-338 , denotes ,
T14522 338-345 NNP denotes Oakland
T14523 345-347 , denotes ,
T14524 347-350 NNP denotes USA
T14525 350-351 -RRB- denotes )
T14526 351-352 . denotes .
T14527 352-638 sentence denotes A 14.5 kb KpnI/BamHI fragment containing the entire Ptdsr locus and 5' and 3' flanking regions was subcloned from this BAC clone and a 1.9 kb RsrII/AatII fragment containing exons I and II of the Ptdsr gene was replaced by a 1.2 kb loxP-flanked neomycin-resistance gene cassette (neo).
T14528 353-354 DT denotes A
T14530 355-359 CD denotes 14.5
T14531 360-362 NN denotes kb
T14532 363-367 NN denotes KpnI
T14534 367-368 HYPH denotes /
T14533 368-373 NN denotes BamHI
T14529 374-382 NN denotes fragment
T14536 383-393 VBG denotes containing
T14537 394-397 DT denotes the
T14539 398-404 JJ denotes entire
T14540 405-410 NN denotes Ptdsr
T14538 411-416 NN denotes locus
T14541 417-420 CC denotes and
T14542 421-422 CD denotes 5
T14544 422-423 SYM denotes '
T14545 424-427 CC denotes and
T14546 428-429 CD denotes 3
T14547 429-430 SYM denotes '
T14548 431-439 NN denotes flanking
T14543 440-447 NNS denotes regions
T14549 448-451 VBD denotes was
T14535 452-461 VBN denotes subcloned
T14550 462-466 IN denotes from
T14551 467-471 DT denotes this
T14553 472-475 NN denotes BAC
T14552 476-481 NN denotes clone
T14554 482-485 CC denotes and
T14555 486-487 DT denotes a
T14557 488-491 CD denotes 1.9
T14558 492-494 NN denotes kb
T14559 495-500 NN denotes RsrII
T14561 500-501 HYPH denotes /
T14560 501-506 NN denotes AatII
T14556 507-515 NN denotes fragment
T14563 516-526 VBG denotes containing
T14564 527-532 NNS denotes exons
T14565 533-534 CD denotes I
T14566 535-538 CC denotes and
T14567 539-541 CD denotes II
T14568 542-544 IN denotes of
T14569 545-548 DT denotes the
T14571 549-554 NN denotes Ptdsr
T14570 555-559 NN denotes gene
T14572 560-563 VBD denotes was
T14562 564-572 VBN denotes replaced
T14573 573-575 IN denotes by
T14574 576-577 DT denotes a
T14576 578-581 CD denotes 1.2
T14577 582-584 NN denotes kb
T14578 585-589 NN denotes loxP
T14580 589-590 HYPH denotes -
T14579 590-597 VBN denotes flanked
T14581 598-606 NN denotes neomycin
T14583 606-607 HYPH denotes -
T14582 607-617 NN denotes resistance
T14584 618-622 NN denotes gene
T14575 623-631 NN denotes cassette
T14585 632-633 -LRB- denotes (
T14586 633-636 NN denotes neo
T14587 636-637 -RRB- denotes )
T14588 637-638 . denotes .
T14589 638-713 sentence denotes Homologous recombination in ES cells and generation of germ-line chimeras
T14590 640-650 JJ denotes Homologous
T14591 651-664 NN denotes recombination
T14592 665-667 IN denotes in
T14593 668-670 NN denotes ES
T14594 671-676 NNS denotes cells
T14595 677-680 CC denotes and
T14596 681-691 NN denotes generation
T14597 692-694 IN denotes of
T14598 695-699 NN denotes germ
T14600 699-700 HYPH denotes -
T14599 700-704 NN denotes line
T14601 705-713 NNS denotes chimeras
T14602 713-808 sentence denotes Bruce4 ES cells were transfected with KpnI-linearized targeting vector and selected with G418.
T14603 714-720 NN denotes Bruce4
T14605 721-723 NN denotes ES
T14604 724-729 NNS denotes cells
T14607 730-734 VBD denotes were
T14606 735-746 VBN denotes transfected
T14608 747-751 IN denotes with
T14609 752-756 NN denotes KpnI
T14611 756-757 HYPH denotes -
T14610 757-767 VBN denotes linearized
T14613 768-777 NN denotes targeting
T14612 778-784 NN denotes vector
T14614 785-788 CC denotes and
T14615 789-797 VBN denotes selected
T14616 798-802 IN denotes with
T14617 803-807 NN denotes G418
T14618 807-808 . denotes .
T14619 808-954 sentence denotes ES-cell clones resistant to G418 were isolated and analyzed by Southern blot analysis for homologous recombination events within the Ptdsr locus.
T14620 809-811 NN denotes ES
T14622 811-812 HYPH denotes -
T14621 812-816 NN denotes cell
T14623 817-823 NNS denotes clones
T14625 824-833 JJ denotes resistant
T14626 834-836 IN denotes to
T14627 837-841 NN denotes G418
T14628 842-846 VBD denotes were
T14624 847-855 VBN denotes isolated
T14629 856-859 CC denotes and
T14630 860-868 VBN denotes analyzed
T14631 869-871 IN denotes by
T14632 872-880 NNP denotes Southern
T14633 881-885 NN denotes blot
T14634 886-894 NN denotes analysis
T14635 895-898 IN denotes for
T14636 899-909 JJ denotes homologous
T14638 910-923 NN denotes recombination
T14637 924-930 NNS denotes events
T14639 931-937 IN denotes within
T14640 938-941 DT denotes the
T14642 942-947 NN denotes Ptdsr
T14641 948-953 NN denotes locus
T14643 953-954 . denotes .
T14644 954-1165 sentence denotes Chimeric mice were produced by microinjection of two independent homologous recombinant (Ptdsr+/-) ES cells into BALB/c blastocysts and transfer to pseudopregnant foster mothers followed by development to term.
T14645 955-963 JJ denotes Chimeric
T14646 964-968 NNS denotes mice
T14648 969-973 VBD denotes were
T14647 974-982 VBN denotes produced
T14649 983-985 IN denotes by
T14650 986-1000 NN denotes microinjection
T14651 1001-1003 IN denotes of
T14652 1004-1007 CD denotes two
T14654 1008-1019 JJ denotes independent
T14655 1020-1030 JJ denotes homologous
T14656 1031-1042 JJ denotes recombinant
T14657 1043-1044 -LRB- denotes (
T14658 1044-1049 NN denotes Ptdsr
T14659 1049-1050 SYM denotes +
T14660 1050-1051 HYPH denotes /
T14661 1051-1052 SYM denotes -
T14662 1052-1053 -RRB- denotes )
T14663 1054-1056 NN denotes ES
T14653 1057-1062 NNS denotes cells
T14664 1063-1067 IN denotes into
T14665 1068-1072 NN denotes BALB
T14667 1072-1073 HYPH denotes /
T14666 1073-1074 NN denotes c
T14668 1075-1086 NNS denotes blastocysts
T14669 1087-1090 CC denotes and
T14670 1091-1099 NN denotes transfer
T14671 1100-1102 IN denotes to
T14672 1103-1117 JJ denotes pseudopregnant
T14674 1118-1124 JJ denotes foster
T14673 1125-1132 NNS denotes mothers
T14675 1133-1141 VBN denotes followed
T14676 1142-1144 IN denotes by
T14677 1145-1156 NN denotes development
T14678 1157-1159 IN denotes to
T14679 1160-1164 NN denotes term
T14680 1164-1165 . denotes .
T14681 1165-1214 sentence denotes Chimeric males were mated with C57BL/6J females.
T14682 1166-1174 JJ denotes Chimeric
T14683 1175-1180 NNS denotes males
T14685 1181-1185 VBD denotes were
T14684 1186-1191 VBN denotes mated
T14686 1192-1196 IN denotes with
T14687 1197-1202 NN denotes C57BL
T14689 1202-1203 HYPH denotes /
T14688 1203-1205 NN denotes 6J
T14690 1206-1213 NNS denotes females
T14691 1213-1214 . denotes .
T14692 1214-1299 sentence denotes From the two selected ES-cell clones, one successfully contributed to the germ-line.
T14693 1215-1219 IN denotes From
T14695 1220-1223 DT denotes the
T14697 1224-1227 CD denotes two
T14698 1228-1236 VBN denotes selected
T14699 1237-1239 NN denotes ES
T14701 1239-1240 HYPH denotes -
T14700 1240-1244 NN denotes cell
T14696 1245-1251 NNS denotes clones
T14702 1251-1253 , denotes ,
T14703 1253-1256 CD denotes one
T14704 1257-1269 RB denotes successfully
T14694 1270-1281 VBD denotes contributed
T14705 1282-1284 IN denotes to
T14706 1285-1288 DT denotes the
T14708 1289-1293 NN denotes germ
T14709 1293-1294 HYPH denotes -
T14707 1294-1298 NN denotes line
T14710 1298-1299 . denotes .
T14711 1299-1432 sentence denotes Germ-line transmission of the mutant allele was verified by PCR and Southern blot of genomic DNA from black coat-color F1 offspring.
T14712 1300-1304 NN denotes Germ
T14714 1304-1305 HYPH denotes -
T14713 1305-1309 NN denotes line
T14715 1310-1322 NN denotes transmission
T14717 1323-1325 IN denotes of
T14718 1326-1329 DT denotes the
T14720 1330-1336 JJ denotes mutant
T14719 1337-1343 NN denotes allele
T14721 1344-1347 VBD denotes was
T14716 1348-1356 VBN denotes verified
T14722 1357-1359 IN denotes by
T14723 1360-1363 NN denotes PCR
T14724 1364-1367 CC denotes and
T14725 1368-1376 NNP denotes Southern
T14726 1377-1381 NN denotes blot
T14727 1382-1384 IN denotes of
T14728 1385-1392 JJ denotes genomic
T14729 1393-1396 NN denotes DNA
T14730 1397-1401 IN denotes from
T14731 1402-1407 JJ denotes black
T14733 1408-1412 NN denotes coat
T14734 1412-1413 HYPH denotes -
T14732 1413-1418 NN denotes color
T14736 1419-1421 NN denotes F1
T14735 1422-1431 NN denotes offspring
T14737 1431-1432 . denotes .
T14738 1432-1486 sentence denotes Ptdsr gene-trap and generation of germ-line chimeras
T14739 1434-1439 NN denotes Ptdsr
T14741 1440-1444 NN denotes gene
T14742 1444-1445 HYPH denotes -
T14740 1445-1449 NN denotes trap
T14743 1450-1453 CC denotes and
T14744 1454-1464 NN denotes generation
T14745 1465-1467 IN denotes of
T14746 1468-1472 NN denotes germ
T14748 1472-1473 HYPH denotes -
T14747 1473-1477 NN denotes line
T14749 1478-1486 NNS denotes chimeras
T14750 1486-1681 sentence denotes An ES-cell line carrying a β-geo gene-trap vector in the Ptdsr locus was identified by searching the BayGenomics database (BayGenomics, San Francisco, USA; [48]) with the full-length Ptdsr cDNA.
T14751 1487-1489 DT denotes An
T14753 1490-1492 NN denotes ES
T14755 1492-1493 HYPH denotes -
T14754 1493-1497 NN denotes cell
T14752 1498-1502 NN denotes line
T14757 1503-1511 VBG denotes carrying
T14758 1512-1513 DT denotes a
T14760 1514-1515 NN denotes β
T14762 1515-1516 HYPH denotes -
T14761 1516-1519 NN denotes geo
T14763 1520-1524 NN denotes gene
T14765 1524-1525 HYPH denotes -
T14764 1525-1529 NN denotes trap
T14759 1530-1536 NN denotes vector
T14766 1537-1539 IN denotes in
T14767 1540-1543 DT denotes the
T14769 1544-1549 NN denotes Ptdsr
T14768 1550-1555 NN denotes locus
T14770 1556-1559 VBD denotes was
T14756 1560-1570 VBN denotes identified
T14771 1571-1573 IN denotes by
T14772 1574-1583 VBG denotes searching
T14773 1584-1587 DT denotes the
T14775 1588-1599 NNP denotes BayGenomics
T14774 1600-1608 NN denotes database
T14776 1609-1610 -LRB- denotes (
T14778 1610-1621 NNP denotes BayGenomics
T14779 1621-1623 , denotes ,
T14780 1623-1626 NNP denotes San
T14781 1627-1636 NNP denotes Francisco
T14782 1636-1638 , denotes ,
T14783 1638-1641 NNP denotes USA
T14784 1641-1642 : denotes ;
T14785 1643-1644 -LRB- denotes [
T14777 1644-1646 CD denotes 48
T14786 1646-1647 -RRB- denotes ]
T14787 1647-1648 -RRB- denotes )
T14788 1649-1653 IN denotes with
T14789 1654-1657 DT denotes the
T14791 1658-1662 JJ denotes full
T14793 1662-1663 HYPH denotes -
T14792 1663-1669 NN denotes length
T14794 1670-1675 NN denotes Ptdsr
T14790 1676-1680 NN denotes cDNA
T14795 1680-1681 . denotes .
T14796 1681-1796 sentence denotes A single ES-cell line was identified carrying the gene-trap in intron V, between exons V and VI of the Ptdsr gene.
T14797 1682-1683 DT denotes A
T14799 1684-1690 JJ denotes single
T14800 1691-1693 NN denotes ES
T14802 1693-1694 HYPH denotes -
T14801 1694-1698 NN denotes cell
T14798 1699-1703 NN denotes line
T14804 1704-1707 VBD denotes was
T14805 1708-1718 VBN denotes identified
T14803 1719-1727 VBG denotes carrying
T14806 1728-1731 DT denotes the
T14808 1732-1736 NN denotes gene
T14809 1736-1737 HYPH denotes -
T14807 1737-1741 NN denotes trap
T14810 1742-1744 IN denotes in
T14811 1745-1751 NN denotes intron
T14812 1752-1753 CD denotes V
T14813 1753-1755 , denotes ,
T14814 1755-1762 IN denotes between
T14815 1763-1768 NNS denotes exons
T14816 1769-1770 CD denotes V
T14817 1771-1774 CC denotes and
T14818 1775-1777 CD denotes VI
T14819 1778-1780 IN denotes of
T14820 1781-1784 DT denotes the
T14822 1785-1790 NN denotes Ptdsr
T14821 1791-1795 NN denotes gene
T14823 1795-1796 . denotes .
T14824 1796-1912 sentence denotes Chimeric mice were generated by microinjection into CB20 blastocysts and transfer to pseudopregnant foster mothers.
T14825 1797-1805 JJ denotes Chimeric
T14826 1806-1810 NNS denotes mice
T14828 1811-1815 VBD denotes were
T14827 1816-1825 VBN denotes generated
T14829 1826-1828 IN denotes by
T14830 1829-1843 NN denotes microinjection
T14831 1844-1848 IN denotes into
T14832 1849-1853 NN denotes CB20
T14833 1854-1865 NNS denotes blastocysts
T14834 1866-1869 CC denotes and
T14835 1870-1878 NN denotes transfer
T14836 1879-1881 IN denotes to
T14837 1882-1896 JJ denotes pseudopregnant
T14839 1897-1903 JJ denotes foster
T14838 1904-1911 NNS denotes mothers
T14840 1911-1912 . denotes .
T14841 1912-1965 sentence denotes Chimeric males were mated with 129P2/OlaHsd females.
T14842 1913-1921 JJ denotes Chimeric
T14843 1922-1927 NNS denotes males
T14845 1928-1932 VBD denotes were
T14844 1933-1938 VBN denotes mated
T14846 1939-1943 IN denotes with
T14847 1944-1949 NN denotes 129P2
T14849 1949-1950 HYPH denotes /
T14848 1950-1956 NN denotes OlaHsd
T14850 1957-1964 NNS denotes females
T14851 1964-1965 . denotes .
T14852 1965-2098 sentence denotes Germ-line transmission of the mutant gene-trap allele was verified by expression analysis using β-galactosidase staining and RT-PCR.
T14853 1966-1970 NN denotes Germ
T14855 1970-1971 HYPH denotes -
T14854 1971-1975 NN denotes line
T14856 1976-1988 NN denotes transmission
T14858 1989-1991 IN denotes of
T14859 1992-1995 DT denotes the
T14861 1996-2002 JJ denotes mutant
T14862 2003-2007 NN denotes gene
T14864 2007-2008 HYPH denotes -
T14863 2008-2012 NN denotes trap
T14860 2013-2019 NN denotes allele
T14865 2020-2023 VBD denotes was
T14857 2024-2032 VBN denotes verified
T14866 2033-2035 IN denotes by
T14867 2036-2046 NN denotes expression
T14868 2047-2055 NN denotes analysis
T14869 2056-2061 VBG denotes using
T14870 2062-2063 NN denotes β
T14872 2063-2064 HYPH denotes -
T14871 2064-2077 NN denotes galactosidase
T14873 2078-2086 NN denotes staining
T14874 2087-2090 CC denotes and
T14875 2091-2093 NN denotes RT
T14877 2093-2094 HYPH denotes -
T14876 2094-2097 NN denotes PCR
T14878 2097-2098 . denotes .
T15029 2100-2108 NN denotes Genotype
T15030 2109-2117 NN denotes analysis
T15031 2117-2217 sentence denotes The genotypes of embryos or animals were determined by PCR analysis and confirmed by Southern blot.
T15032 2118-2121 DT denotes The
T15033 2122-2131 NNS denotes genotypes
T15035 2132-2134 IN denotes of
T15036 2135-2142 NNS denotes embryos
T15037 2143-2145 CC denotes or
T15038 2146-2153 NNS denotes animals
T15039 2154-2158 VBD denotes were
T15034 2159-2169 VBN denotes determined
T15040 2170-2172 IN denotes by
T15041 2173-2176 NN denotes PCR
T15042 2177-2185 NN denotes analysis
T15043 2186-2189 CC denotes and
T15044 2190-2199 VBN denotes confirmed
T15045 2200-2202 IN denotes by
T15046 2203-2211 NNP denotes Southern
T15047 2212-2216 NN denotes blot
T15048 2216-2217 . denotes .
T15049 2217-2349 sentence denotes Genomic DNA for PCR was prepared from extraembryonic membranes or tail clips using a non-organic tail-DNA extraction protocol [49].
T15050 2218-2225 JJ denotes Genomic
T15051 2226-2229 NN denotes DNA
T15053 2230-2233 IN denotes for
T15054 2234-2237 NN denotes PCR
T15055 2238-2241 VBD denotes was
T15052 2242-2250 VBN denotes prepared
T15056 2251-2255 IN denotes from
T15057 2256-2270 JJ denotes extraembryonic
T15058 2271-2280 NNS denotes membranes
T15059 2281-2283 CC denotes or
T15060 2284-2288 NN denotes tail
T15061 2289-2294 NNS denotes clips
T15062 2295-2300 VBG denotes using
T15063 2301-2302 DT denotes a
T15065 2303-2314 JJ denotes non-organic
T15066 2315-2319 NN denotes tail
T15068 2319-2320 HYPH denotes -
T15067 2320-2323 NN denotes DNA
T15069 2324-2334 NN denotes extraction
T15064 2335-2343 NN denotes protocol
T15070 2344-2345 -LRB- denotes [
T15071 2345-2347 CD denotes 49
T15072 2347-2348 -RRB- denotes ]
T15073 2348-2349 . denotes .
T15074 2349-2451 sentence denotes High molecular weight genomic DNA for Southern blotting was prepared according to standard protocols.
T15075 2350-2354 JJ denotes High
T15077 2355-2364 JJ denotes molecular
T15076 2365-2371 NN denotes weight
T15079 2372-2379 JJ denotes genomic
T15078 2380-2383 NN denotes DNA
T15081 2384-2387 IN denotes for
T15082 2388-2396 NNP denotes Southern
T15083 2397-2405 NN denotes blotting
T15084 2406-2409 VBD denotes was
T15080 2410-2418 VBN denotes prepared
T15085 2419-2428 VBG denotes according
T15086 2429-2431 IN denotes to
T15087 2432-2440 JJ denotes standard
T15088 2441-2450 NNS denotes protocols
T15089 2450-2451 . denotes .
T15090 2451-2614 sentence denotes For PCR analysis the wild-type Ptdsr allele was detected using forward primer 1 (5'-GACACTGTCCATGGCAAACAC-3') and reverse primer 2 (5'-TAAAGTCGCCTTCCAGAAGATT-3').
T15091 2452-2455 IN denotes For
T15093 2456-2459 NN denotes PCR
T15094 2460-2468 NN denotes analysis
T15095 2469-2472 DT denotes the
T15097 2473-2477 JJ denotes wild
T15099 2477-2478 HYPH denotes -
T15098 2478-2482 NN denotes type
T15100 2483-2488 NN denotes Ptdsr
T15096 2489-2495 NN denotes allele
T15101 2496-2499 VBD denotes was
T15092 2500-2508 VBN denotes detected
T15102 2509-2514 VBG denotes using
T15103 2515-2522 RB denotes forward
T15104 2523-2529 NN denotes primer
T15105 2530-2531 CD denotes 1
T15106 2532-2533 -LRB- denotes (
T15108 2533-2534 CD denotes 5
T15109 2534-2535 SYM denotes '
T15110 2535-2536 HYPH denotes -
T15107 2536-2557 NN denotes GACACTGTCCATGGCAAACAC
T15111 2557-2558 HYPH denotes -
T15112 2558-2559 CD denotes 3
T15113 2559-2560 SYM denotes '
T15114 2560-2561 -RRB- denotes )
T15115 2562-2565 CC denotes and
T15116 2566-2573 JJ denotes reverse
T15117 2574-2580 NN denotes primer
T15118 2581-2582 CD denotes 2
T15119 2583-2584 -LRB- denotes (
T15121 2584-2585 CD denotes 5
T15122 2585-2586 SYM denotes '
T15123 2586-2587 HYPH denotes -
T15120 2587-2609 NN denotes TAAAGTCGCCTTCCAGAAGATT
T15124 2609-2610 HYPH denotes -
T15125 2610-2611 CD denotes 3
T15126 2611-2612 SYM denotes '
T15127 2612-2613 -RRB- denotes )
T15128 2613-2614 . denotes .
T15129 2614-2705 sentence denotes The primer 1 site is located 5' to the deletion and the primer 2 site within the deletion.
T15130 2615-2618 DT denotes The
T15132 2619-2625 NN denotes primer
T15133 2626-2627 CD denotes 1
T15131 2628-2632 NN denotes site
T15135 2633-2635 VBZ denotes is
T15134 2636-2643 VBN denotes located
T15136 2644-2645 CD denotes 5
T15137 2645-2646 SYM denotes '
T15138 2647-2649 IN denotes to
T15139 2650-2653 DT denotes the
T15140 2654-2662 NN denotes deletion
T15141 2663-2666 CC denotes and
T15142 2667-2670 DT denotes the
T15144 2671-2677 NN denotes primer
T15145 2678-2679 CD denotes 2
T15143 2680-2684 NN denotes site
T15146 2685-2691 IN denotes within
T15147 2692-2695 DT denotes the
T15148 2696-2704 NN denotes deletion
T15149 2704-2705 . denotes .
T15150 2705-2831 sentence denotes This primer pair amplified a fragment of approximately 300 bp from wild-type and Ptdsr+/- mice but not from Ptdsr -/-mutants.
T15151 2706-2710 DT denotes This
T15153 2711-2717 NN denotes primer
T15152 2718-2722 NN denotes pair
T15154 2723-2732 VBD denotes amplified
T15155 2733-2734 DT denotes a
T15156 2735-2743 NN denotes fragment
T15157 2744-2746 IN denotes of
T15158 2747-2760 RB denotes approximately
T15159 2761-2764 CD denotes 300
T15160 2765-2767 NNS denotes bp
T15161 2768-2772 IN denotes from
T15162 2773-2777 JJ denotes wild
T15164 2777-2778 HYPH denotes -
T15163 2778-2782 NN denotes type
T15166 2783-2786 CC denotes and
T15167 2787-2792 NN denotes Ptdsr
T15168 2792-2793 SYM denotes +
T15169 2793-2794 HYPH denotes /
T15170 2794-2795 SYM denotes -
T15165 2796-2800 NNS denotes mice
T15171 2801-2804 CC denotes but
T15172 2805-2808 RB denotes not
T15173 2809-2813 IN denotes from
T15174 2814-2819 NN denotes Ptdsr
T15176 2820-2821 SYM denotes -
T15177 2821-2822 HYPH denotes /
T15178 2822-2823 SYM denotes -
T15175 2823-2830 NNS denotes mutants
T15179 2830-2831 . denotes .
T15180 2831-3016 sentence denotes To detect the mutant Ptdsr allele, genomic DNA was also amplified using primer 1 and reverse primer 3 (5'-CCACACGCGTCACCTTAATA-3'), which corresponds to a sequence in the neo cassette.
T15181 2832-2834 TO denotes To
T15182 2835-2841 VB denotes detect
T15184 2842-2845 DT denotes the
T15186 2846-2852 JJ denotes mutant
T15187 2853-2858 NN denotes Ptdsr
T15185 2859-2865 NN denotes allele
T15188 2865-2867 , denotes ,
T15189 2867-2874 JJ denotes genomic
T15190 2875-2878 NN denotes DNA
T15191 2879-2882 VBD denotes was
T15192 2883-2887 RB denotes also
T15183 2888-2897 VBN denotes amplified
T15193 2898-2903 VBG denotes using
T15194 2904-2910 NN denotes primer
T15195 2911-2912 CD denotes 1
T15196 2913-2916 CC denotes and
T15197 2917-2924 JJ denotes reverse
T15198 2925-2931 NN denotes primer
T15199 2932-2933 CD denotes 3
T15200 2934-2935 -LRB- denotes (
T15202 2935-2936 CD denotes 5
T15203 2936-2937 SYM denotes '
T15204 2937-2938 HYPH denotes -
T15201 2938-2958 NN denotes CCACACGCGTCACCTTAATA
T15205 2958-2959 HYPH denotes -
T15206 2959-2960 CD denotes 3
T15207 2960-2961 SYM denotes '
T15208 2961-2962 -RRB- denotes )
T15209 2962-2964 , denotes ,
T15210 2964-2969 WDT denotes which
T15211 2970-2981 VBZ denotes corresponds
T15212 2982-2984 IN denotes to
T15213 2985-2986 DT denotes a
T15214 2987-2995 NN denotes sequence
T15215 2996-2998 IN denotes in
T15216 2999-3002 DT denotes the
T15218 3003-3006 NN denotes neo
T15217 3007-3015 NN denotes cassette
T15219 3015-3016 . denotes .
T15220 3016-3167 sentence denotes In this case, a 500 bp fragment was detected in mice heterozygous or homozygous for the mutant allele, while no signal was detected in wild-type mice.
T15221 3017-3019 IN denotes In
T15223 3020-3024 DT denotes this
T15224 3025-3029 NN denotes case
T15225 3029-3031 , denotes ,
T15226 3031-3032 DT denotes a
T15228 3033-3036 CD denotes 500
T15229 3037-3039 NN denotes bp
T15227 3040-3048 NN denotes fragment
T15230 3049-3052 VBD denotes was
T15222 3053-3061 VBN denotes detected
T15231 3062-3064 IN denotes in
T15232 3065-3069 NNS denotes mice
T15233 3070-3082 JJ denotes heterozygous
T15234 3083-3085 CC denotes or
T15235 3086-3096 JJ denotes homozygous
T15236 3097-3100 IN denotes for
T15237 3101-3104 DT denotes the
T15239 3105-3111 JJ denotes mutant
T15238 3112-3118 NN denotes allele
T15240 3118-3120 , denotes ,
T15241 3120-3125 IN denotes while
T15243 3126-3128 DT denotes no
T15244 3129-3135 NN denotes signal
T15245 3136-3139 VBD denotes was
T15242 3140-3148 VBN denotes detected
T15246 3149-3151 IN denotes in
T15247 3152-3156 JJ denotes wild
T15249 3156-3157 HYPH denotes -
T15248 3157-3161 NN denotes type
T15250 3162-3166 NNS denotes mice
T15251 3166-3167 . denotes .
T15252 3167-3503 sentence denotes For Southern blot analysis, genomic DNA (30 μg) was digested overnight with BamHI (30 U; Roche Diagnostics GmbH, Mannheim, Germany) and ScaI (30 U; Roche), fractionated on a 0.8 % agarose gel, transferred to a nylon membrane (Hybond N; Amersham Biosciences Europe GmbH, Freiburg, Germany) and hybridized with 5' and 3' flanking probes.
T15253 3168-3171 IN denotes For
T15255 3172-3180 NNP denotes Southern
T15256 3181-3185 NN denotes blot
T15257 3186-3194 NN denotes analysis
T15258 3194-3196 , denotes ,
T15259 3196-3203 JJ denotes genomic
T15260 3204-3207 NN denotes DNA
T15261 3208-3209 -LRB- denotes (
T15263 3209-3211 CD denotes 30
T15262 3212-3214 NNS denotes μg
T15264 3214-3215 -RRB- denotes )
T15265 3216-3219 VBD denotes was
T15254 3220-3228 VBN denotes digested
T15266 3229-3238 RB denotes overnight
T15267 3239-3243 IN denotes with
T15268 3244-3249 NN denotes BamHI
T15269 3250-3251 -LRB- denotes (
T15271 3251-3253 CD denotes 30
T15272 3254-3255 NN denotes U
T15273 3255-3256 : denotes ;
T15274 3257-3262 NNP denotes Roche
T15275 3263-3274 NNP denotes Diagnostics
T15270 3275-3279 NNP denotes GmbH
T15276 3279-3281 , denotes ,
T15277 3281-3289 NNP denotes Mannheim
T15278 3289-3291 , denotes ,
T15279 3291-3298 NNP denotes Germany
T15280 3298-3299 -RRB- denotes )
T15281 3300-3303 CC denotes and
T15282 3304-3308 NN denotes ScaI
T15283 3309-3310 -LRB- denotes (
T15285 3310-3312 CD denotes 30
T15286 3313-3314 NN denotes U
T15287 3314-3315 : denotes ;
T15284 3316-3321 NNP denotes Roche
T15288 3321-3322 -RRB- denotes )
T15289 3322-3324 , denotes ,
T15290 3324-3336 VBN denotes fractionated
T15291 3337-3339 IN denotes on
T15292 3340-3341 DT denotes a
T15294 3342-3345 CD denotes 0.8
T15295 3346-3347 NN denotes %
T15296 3348-3355 NN denotes agarose
T15293 3356-3359 NN denotes gel
T15297 3359-3361 , denotes ,
T15298 3361-3372 VBN denotes transferred
T15299 3373-3375 IN denotes to
T15300 3376-3377 DT denotes a
T15302 3378-3383 NN denotes nylon
T15301 3384-3392 NN denotes membrane
T15303 3393-3394 -LRB- denotes (
T15305 3394-3400 NN denotes Hybond
T15306 3401-3402 NN denotes N
T15307 3402-3403 : denotes ;
T15308 3404-3412 NNP denotes Amersham
T15309 3413-3424 NNP denotes Biosciences
T15310 3425-3431 NNP denotes Europe
T15304 3432-3436 NNP denotes GmbH
T15311 3436-3438 , denotes ,
T15312 3438-3446 NNP denotes Freiburg
T15313 3446-3448 , denotes ,
T15314 3448-3455 NNP denotes Germany
T15315 3455-3456 -RRB- denotes )
T15316 3457-3460 CC denotes and
T15317 3461-3471 VBN denotes hybridized
T15318 3472-3476 IN denotes with
T15319 3477-3478 CD denotes 5
T15321 3478-3479 SYM denotes '
T15322 3480-3483 CC denotes and
T15323 3484-3485 CD denotes 3
T15324 3485-3486 SYM denotes '
T15325 3487-3495 NN denotes flanking
T15320 3496-3502 NNS denotes probes
T15326 3502-3503 . denotes .
T15327 3503-3723 sentence denotes The BamHI digest was hybridized with a Ptdsr-specific 5' flanking probe, and Southern blot gave a single 17.2 kb band for wild-type (+/+), an 11.6 kb band for homozygous (-/-) and both bands for heterozygous (+/-) mice.
T15328 3504-3507 DT denotes The
T15330 3508-3513 NN denotes BamHI
T15329 3514-3520 NN denotes digest
T15332 3521-3524 VBD denotes was
T15331 3525-3535 VBN denotes hybridized
T15333 3536-3540 IN denotes with
T15334 3541-3542 DT denotes a
T15336 3543-3548 NN denotes Ptdsr
T15338 3548-3549 HYPH denotes -
T15337 3549-3557 JJ denotes specific
T15339 3558-3559 CD denotes 5
T15340 3559-3560 SYM denotes '
T15341 3561-3569 NN denotes flanking
T15335 3570-3575 NN denotes probe
T15342 3575-3577 , denotes ,
T15343 3577-3580 CC denotes and
T15344 3581-3589 NNP denotes Southern
T15345 3590-3594 NN denotes blot
T15346 3595-3599 VBD denotes gave
T15347 3600-3601 DT denotes a
T15349 3602-3608 JJ denotes single
T15350 3609-3613 CD denotes 17.2
T15351 3614-3616 NN denotes kb
T15348 3617-3621 NN denotes band
T15352 3622-3625 IN denotes for
T15353 3626-3630 JJ denotes wild
T15355 3630-3631 HYPH denotes -
T15354 3631-3635 NN denotes type
T15356 3636-3637 -LRB- denotes (
T15358 3637-3638 SYM denotes +
T15359 3638-3639 HYPH denotes /
T15357 3639-3640 SYM denotes +
T15360 3640-3641 -RRB- denotes )
T15361 3641-3643 , denotes ,
T15362 3643-3645 DT denotes an
T15364 3646-3650 CD denotes 11.6
T15365 3651-3653 NN denotes kb
T15363 3654-3658 NN denotes band
T15366 3659-3662 IN denotes for
T15367 3663-3673 JJ denotes homozygous
T15368 3674-3675 -LRB- denotes (
T15370 3675-3676 SYM denotes -
T15371 3676-3677 HYPH denotes /
T15369 3677-3678 SYM denotes -
T15372 3678-3679 -RRB- denotes )
T15373 3680-3683 CC denotes and
T15374 3684-3688 DT denotes both
T15375 3689-3694 NNS denotes bands
T15377 3695-3698 IN denotes for
T15378 3699-3711 JJ denotes heterozygous
T15379 3712-3713 -LRB- denotes (
T15381 3713-3714 SYM denotes +
T15382 3714-3715 HYPH denotes /
T15380 3715-3716 SYM denotes -
T15383 3716-3717 -RRB- denotes )
T15376 3718-3722 NNS denotes mice
T15384 3722-3723 . denotes .
T15385 3723-3909 sentence denotes The ScaI digest was hybridized using a 3' flanking probe, and Southern blot gave a single 12.4 kb band for wild-type, a 17.2 kb band for homozygous and both bands for heterozygous mice.
T15386 3724-3727 DT denotes The
T15388 3728-3732 NN denotes ScaI
T15387 3733-3739 NN denotes digest
T15390 3740-3743 VBD denotes was
T15389 3744-3754 VBN denotes hybridized
T15391 3755-3760 VBG denotes using
T15392 3761-3762 DT denotes a
T15394 3763-3764 CD denotes 3
T15395 3764-3765 SYM denotes '
T15396 3766-3774 NN denotes flanking
T15393 3775-3780 NN denotes probe
T15397 3780-3782 , denotes ,
T15398 3782-3785 CC denotes and
T15399 3786-3794 NNP denotes Southern
T15400 3795-3799 NN denotes blot
T15401 3800-3804 VBD denotes gave
T15402 3805-3806 DT denotes a
T15404 3807-3813 JJ denotes single
T15405 3814-3818 CD denotes 12.4
T15406 3819-3821 NN denotes kb
T15403 3822-3826 NN denotes band
T15407 3827-3830 IN denotes for
T15408 3831-3835 JJ denotes wild
T15410 3835-3836 HYPH denotes -
T15409 3836-3840 NN denotes type
T15411 3840-3842 , denotes ,
T15412 3842-3843 DT denotes a
T15414 3844-3848 CD denotes 17.2
T15415 3849-3851 NN denotes kb
T15413 3852-3856 NN denotes band
T15416 3857-3860 IN denotes for
T15417 3861-3871 JJ denotes homozygous
T15418 3872-3875 CC denotes and
T15419 3876-3880 DT denotes both
T15420 3881-3886 NNS denotes bands
T15421 3887-3890 IN denotes for
T15422 3891-3903 JJ denotes heterozygous
T15423 3904-3908 NNS denotes mice
T15424 3908-3909 . denotes .
T15486 3911-3919 NNP denotes Northern
T15487 3920-3924 NN denotes blot
T15488 3925-3933 NN denotes analysis
T15489 3933-4041 sentence denotes Total RNA was isolated from homogenized embryos using TRIZOL reagent (Invitrogen GmbH, Karlsruhe, Germany).
T15490 3934-3939 JJ denotes Total
T15491 3940-3943 NN denotes RNA
T15493 3944-3947 VBD denotes was
T15492 3948-3956 VBN denotes isolated
T15494 3957-3961 IN denotes from
T15495 3962-3973 VBN denotes homogenized
T15496 3974-3981 NNS denotes embryos
T15497 3982-3987 VBG denotes using
T15498 3988-3994 NN denotes TRIZOL
T15499 3995-4002 NN denotes reagent
T15500 4003-4004 -LRB- denotes (
T15502 4004-4014 NNP denotes Invitrogen
T15501 4015-4019 NNP denotes GmbH
T15503 4019-4021 , denotes ,
T15504 4021-4030 NNP denotes Karlsruhe
T15505 4030-4032 , denotes ,
T15506 4032-4039 NNP denotes Germany
T15507 4039-4040 -RRB- denotes )
T15508 4040-4041 . denotes .
T15509 4041-4437 sentence denotes For northern blots, either total RNA (30 μg) was extracted from embryos, electrophoresed and transferred to a nylon membrane (Hybond N; Amersham) or a polyA+ RNA northern blot (OriGene Technologies Inc., Rockville, USA) was hybridized using as the probe a Ptdsr fragment amplified from wild-type cDNA using forward primer 5'-GTTCCAGCTCGTCAGACTCG-3' and reverse primer 5'-TGCCCCTAAGACATGACCAC-3'.
T15510 4042-4045 IN denotes For
T15512 4046-4054 NNP denotes northern
T15513 4055-4060 NNS denotes blots
T15514 4060-4062 , denotes ,
T15515 4062-4068 CC denotes either
T15516 4069-4074 JJ denotes total
T15517 4075-4078 NN denotes RNA
T15518 4079-4080 -LRB- denotes (
T15520 4080-4082 CD denotes 30
T15519 4083-4085 NN denotes μg
T15521 4085-4086 -RRB- denotes )
T15522 4087-4090 VBD denotes was
T15511 4091-4100 VBN denotes extracted
T15523 4101-4105 IN denotes from
T15524 4106-4113 NNS denotes embryos
T15525 4113-4115 , denotes ,
T15526 4115-4130 VBN denotes electrophoresed
T15527 4131-4134 CC denotes and
T15528 4135-4146 VBN denotes transferred
T15529 4147-4149 IN denotes to
T15530 4150-4151 DT denotes a
T15532 4152-4157 NN denotes nylon
T15531 4158-4166 NN denotes membrane
T15533 4167-4168 -LRB- denotes (
T15535 4168-4174 NNP denotes Hybond
T15534 4175-4176 NN denotes N
T15536 4176-4177 : denotes ;
T15537 4178-4186 NNP denotes Amersham
T15538 4186-4187 -RRB- denotes )
T15539 4188-4190 CC denotes or
T15540 4191-4192 DT denotes a
T15542 4193-4199 NN denotes polyA+
T15543 4200-4203 NN denotes RNA
T15544 4204-4212 NNP denotes northern
T15541 4213-4217 NN denotes blot
T15546 4218-4219 -LRB- denotes (
T15548 4219-4226 NNP denotes OriGene
T15549 4227-4239 NNP denotes Technologies
T15547 4240-4244 NNP denotes Inc.
T15550 4244-4246 , denotes ,
T15551 4246-4255 NNP denotes Rockville
T15552 4255-4257 , denotes ,
T15553 4257-4260 NNP denotes USA
T15554 4260-4261 -RRB- denotes )
T15555 4262-4265 VBD denotes was
T15545 4266-4276 VBN denotes hybridized
T15556 4277-4282 VBG denotes using
T15557 4283-4285 IN denotes as
T15558 4286-4289 DT denotes the
T15559 4290-4295 NN denotes probe
T15560 4296-4297 DT denotes a
T15562 4298-4303 NN denotes Ptdsr
T15561 4304-4312 NN denotes fragment
T15563 4313-4322 VBN denotes amplified
T15564 4323-4327 IN denotes from
T15565 4328-4332 JJ denotes wild
T15567 4332-4333 HYPH denotes -
T15566 4333-4337 NN denotes type
T15568 4338-4342 NN denotes cDNA
T15569 4343-4348 VBG denotes using
T15570 4349-4356 JJ denotes forward
T15571 4357-4363 NN denotes primer
T15573 4364-4365 CD denotes 5
T15574 4365-4366 SYM denotes '
T15575 4366-4367 HYPH denotes -
T15572 4367-4387 NN denotes GTTCCAGCTCGTCAGACTCG
T15576 4387-4388 HYPH denotes -
T15577 4388-4389 CD denotes 3
T15578 4389-4390 SYM denotes '
T15579 4391-4394 CC denotes and
T15580 4395-4402 JJ denotes reverse
T15581 4403-4409 NN denotes primer
T15583 4410-4411 CD denotes 5
T15584 4411-4412 SYM denotes '
T15585 4412-4413 HYPH denotes -
T15582 4413-4433 NN denotes TGCCCCTAAGACATGACCAC
T15586 4433-4434 HYPH denotes -
T15587 4434-4435 CD denotes 3
T15588 4435-4436 SYM denotes '
T15589 4436-4437 . denotes .
T15590 4437-4588 sentence denotes In all experiments the same membrane was re-hybridized with a β-actin probe (OriGene) to confirm that equivalent RNA levels were present in each lane.
T15591 4438-4440 IN denotes In
T15593 4441-4444 DT denotes all
T15594 4445-4456 NNS denotes experiments
T15595 4457-4460 DT denotes the
T15597 4461-4465 JJ denotes same
T15596 4466-4474 NN denotes membrane
T15598 4475-4478 VBD denotes was
T15592 4479-4492 VBN denotes re-hybridized
T15599 4493-4497 IN denotes with
T15600 4498-4499 DT denotes a
T15602 4500-4501 NN denotes β
T15604 4501-4502 HYPH denotes -
T15603 4502-4507 NN denotes actin
T15601 4508-4513 NN denotes probe
T15605 4514-4515 -LRB- denotes (
T15606 4515-4522 NNP denotes OriGene
T15607 4522-4523 -RRB- denotes )
T15608 4524-4526 TO denotes to
T15609 4527-4534 VB denotes confirm
T15610 4535-4539 IN denotes that
T15612 4540-4550 JJ denotes equivalent
T15614 4551-4554 NN denotes RNA
T15613 4555-4561 NNS denotes levels
T15611 4562-4566 VBD denotes were
T15615 4567-4574 JJ denotes present
T15616 4575-4577 IN denotes in
T15617 4578-4582 DT denotes each
T15618 4583-4587 NN denotes lane
T15619 4587-4588 . denotes .
T15620 4588-4752 sentence denotes Northern blotting indicated that homozygous mutant embryos did not express Ptdsr mRNA and heterozygous mutant embryos expressed only reduced amounts of Ptdsr mRNA.
T15621 4589-4597 NNP denotes Northern
T15622 4598-4606 NN denotes blotting
T15623 4607-4616 VBD denotes indicated
T15624 4617-4621 IN denotes that
T15626 4622-4632 JJ denotes homozygous
T15628 4633-4639 JJ denotes mutant
T15627 4640-4647 NNS denotes embryos
T15629 4648-4651 VBD denotes did
T15630 4652-4655 RB denotes not
T15625 4656-4663 VB denotes express
T15631 4664-4669 NN denotes Ptdsr
T15632 4670-4674 NN denotes mRNA
T15633 4675-4678 CC denotes and
T15634 4679-4691 JJ denotes heterozygous
T15636 4692-4698 NN denotes mutant
T15635 4699-4706 NNS denotes embryos
T15637 4707-4716 VBN denotes expressed
T15638 4717-4721 RB denotes only
T15640 4722-4729 VBN denotes reduced
T15639 4730-4737 NNS denotes amounts
T15641 4738-4740 IN denotes of
T15642 4741-4746 NN denotes Ptdsr
T15643 4747-4751 NN denotes mRNA
T15644 4751-4752 . denotes .
T15701 4754-4761 NNP denotes Western
T15702 4762-4766 NN denotes blot
T15703 4767-4775 NN denotes analysis
T15704 4775-4973 sentence denotes Embryos (E13.5) for protein isolation were homogenized in lysis buffer containing 1 × PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor cocktail (CompleteMini; Roche).
T15705 4776-4783 NNS denotes Embryos
T15707 4784-4785 -LRB- denotes (
T15708 4785-4790 NN denotes E13.5
T15709 4790-4791 -RRB- denotes )
T15710 4792-4795 IN denotes for
T15711 4796-4803 NN denotes protein
T15712 4804-4813 NN denotes isolation
T15713 4814-4818 VBD denotes were
T15706 4819-4830 VBN denotes homogenized
T15714 4831-4833 IN denotes in
T15715 4834-4839 NN denotes lysis
T15716 4840-4846 NN denotes buffer
T15717 4847-4857 VBG denotes containing
T15718 4858-4859 CD denotes 1
T15720 4860-4861 SYM denotes ×
T15719 4862-4865 NN denotes PBS
T15721 4865-4867 , denotes ,
T15722 4867-4868 CD denotes 1
T15723 4868-4869 NN denotes %
T15725 4870-4877 NN denotes Nonidet
T15726 4878-4879 NN denotes P
T15727 4879-4880 HYPH denotes -
T15724 4880-4882 NN denotes 40
T15728 4882-4884 , denotes ,
T15729 4884-4887 CD denotes 0.5
T15730 4887-4888 NN denotes %
T15732 4889-4895 NN denotes sodium
T15731 4896-4908 NN denotes deoxycholate
T15733 4908-4910 , denotes ,
T15734 4910-4913 CD denotes 0.1
T15735 4913-4914 NN denotes %
T15736 4915-4918 NN denotes SDS
T15737 4919-4922 CC denotes and
T15738 4923-4931 NN denotes protease
T15740 4932-4941 NN denotes inhibitor
T15739 4942-4950 NN denotes cocktail
T15741 4951-4952 -LRB- denotes (
T15743 4952-4964 NNP denotes CompleteMini
T15744 4964-4965 : denotes ;
T15742 4966-4971 NNP denotes Roche
T15745 4971-4972 -RRB- denotes )
T15746 4972-4973 . denotes .
T15747 4973-5168 sentence denotes Equal amounts (25 μg) of protein lysate were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a PVDF membrane (Millipore, Billerica, USA) according to standard protocols.
T15748 4974-4979 JJ denotes Equal
T15749 4980-4987 NNS denotes amounts
T15751 4988-4989 -LRB- denotes (
T15753 4989-4991 CD denotes 25
T15752 4992-4994 NNS denotes μg
T15754 4994-4995 -RRB- denotes )
T15755 4996-4998 IN denotes of
T15756 4999-5006 NN denotes protein
T15757 5007-5013 NN denotes lysate
T15758 5014-5018 VBD denotes were
T15750 5019-5028 VBN denotes separated
T15759 5029-5031 IN denotes by
T15760 5032-5035 NN denotes SDS
T15762 5035-5036 HYPH denotes -
T15761 5036-5050 NN denotes polyacrylamide
T15764 5051-5054 NN denotes gel
T15763 5055-5070 NN denotes electrophoresis
T15765 5071-5074 CC denotes and
T15766 5075-5086 VBN denotes transferred
T15767 5087-5091 IN denotes onto
T15768 5092-5093 DT denotes a
T15770 5094-5098 NN denotes PVDF
T15769 5099-5107 NN denotes membrane
T15771 5108-5109 -LRB- denotes (
T15772 5109-5118 NNP denotes Millipore
T15773 5118-5120 , denotes ,
T15774 5120-5129 NNP denotes Billerica
T15775 5129-5131 , denotes ,
T15776 5131-5134 NNP denotes USA
T15777 5134-5135 -RRB- denotes )
T15778 5136-5145 VBG denotes according
T15779 5146-5148 IN denotes to
T15780 5149-5157 JJ denotes standard
T15781 5158-5167 NNS denotes protocols
T15782 5167-5168 . denotes .
T15783 5168-5370 sentence denotes Western blots were done using a specific antibody to Ptdsr (PSR N-20, sc-11632; Santa Cruz Biotechnology Inc., Santa Cruz, USA) and β-actin (ab-6276; Abcam, Cambridge, UK) as described by the supplier.
T15784 5169-5176 NNP denotes Western
T15785 5177-5182 NNS denotes blots
T15787 5183-5187 VBD denotes were
T15786 5188-5192 VBN denotes done
T15788 5193-5198 VBG denotes using
T15789 5199-5200 DT denotes a
T15791 5201-5209 JJ denotes specific
T15790 5210-5218 NN denotes antibody
T15792 5219-5221 IN denotes to
T15793 5222-5227 NN denotes Ptdsr
T15794 5228-5229 -LRB- denotes (
T15796 5229-5232 NN denotes PSR
T15797 5233-5234 NN denotes N
T15798 5234-5235 HYPH denotes -
T15799 5235-5237 CD denotes 20
T15800 5237-5239 , denotes ,
T15801 5239-5241 NN denotes sc
T15802 5241-5242 HYPH denotes -
T15803 5242-5247 CD denotes 11632
T15804 5247-5248 : denotes ;
T15805 5249-5254 NNP denotes Santa
T15806 5255-5259 NNP denotes Cruz
T15807 5260-5273 NNP denotes Biotechnology
T15795 5274-5278 NNP denotes Inc.
T15808 5278-5280 , denotes ,
T15809 5280-5285 NNP denotes Santa
T15810 5286-5290 NNP denotes Cruz
T15811 5290-5292 , denotes ,
T15812 5292-5295 NNP denotes USA
T15813 5295-5296 -RRB- denotes )
T15814 5297-5300 CC denotes and
T15815 5301-5302 NN denotes β
T15817 5302-5303 HYPH denotes -
T15816 5303-5308 NN denotes actin
T15818 5309-5310 -LRB- denotes (
T15820 5310-5312 NN denotes ab
T15821 5312-5313 HYPH denotes -
T15822 5313-5317 CD denotes 6276
T15823 5317-5318 : denotes ;
T15819 5319-5324 NNP denotes Abcam
T15824 5324-5326 , denotes ,
T15825 5326-5335 NNP denotes Cambridge
T15826 5335-5337 , denotes ,
T15827 5337-5339 NNP denotes UK
T15828 5339-5340 -RRB- denotes )
T15829 5341-5343 IN denotes as
T15830 5344-5353 VBN denotes described
T15831 5354-5356 IN denotes by
T15832 5357-5360 DT denotes the
T15833 5361-5369 NN denotes supplier
T15834 5369-5370 . denotes .
T15835 5370-5586 sentence denotes Secondary antibodies conjugated to horseradish peroxidase were from Santa Cruz and Abcam, used as described by the supplier, and detection was performed with an enhanced chemiluminescence system (ECLPlus; Amersham).
T15836 5371-5380 JJ denotes Secondary
T15837 5381-5391 NNS denotes antibodies
T15839 5392-5402 VBN denotes conjugated
T15840 5403-5405 IN denotes to
T15841 5406-5417 NN denotes horseradish
T15842 5418-5428 NN denotes peroxidase
T15838 5429-5433 VBD denotes were
T15843 5434-5438 IN denotes from
T15844 5439-5444 NNP denotes Santa
T15845 5445-5449 NNP denotes Cruz
T15846 5450-5453 CC denotes and
T15847 5454-5459 NNP denotes Abcam
T15848 5459-5461 , denotes ,
T15849 5461-5465 VBN denotes used
T15850 5466-5468 IN denotes as
T15851 5469-5478 VBN denotes described
T15852 5479-5481 IN denotes by
T15853 5482-5485 DT denotes the
T15854 5486-5494 NN denotes supplier
T15855 5494-5496 , denotes ,
T15856 5496-5499 CC denotes and
T15857 5500-5509 NN denotes detection
T15859 5510-5513 VBD denotes was
T15858 5514-5523 VBN denotes performed
T15860 5524-5528 IN denotes with
T15861 5529-5531 DT denotes an
T15863 5532-5540 VBN denotes enhanced
T15864 5541-5558 NN denotes chemiluminescence
T15862 5559-5565 NN denotes system
T15865 5566-5567 -LRB- denotes (
T15866 5567-5574 NNP denotes ECLPlus
T15867 5574-5575 : denotes ;
T15868 5576-5584 NNP denotes Amersham
T15869 5584-5585 -RRB- denotes )
T15870 5585-5586 . denotes .
T15896 5588-5594 NN denotes Animal
T15897 5595-5606 NNS denotes experiments
T15898 5606-5749 sentence denotes Wild-type C57BL/6J and 129P2/OlaHsd mice were obtained from Jackson Laboratories (Bar Harbor, USA) and Harlan UK (Bicester, UK), respectively.
T15899 5607-5611 JJ denotes Wild
T15901 5611-5612 HYPH denotes -
T15900 5612-5616 NN denotes type
T15903 5617-5622 NN denotes C57BL
T15905 5622-5623 HYPH denotes /
T15904 5623-5625 NN denotes 6J
T15906 5626-5629 CC denotes and
T15907 5630-5635 NN denotes 129P2
T15909 5635-5636 HYPH denotes /
T15908 5636-5642 NN denotes OlaHsd
T15902 5643-5647 NNS denotes mice
T15911 5648-5652 VBD denotes were
T15910 5653-5661 VBN denotes obtained
T15912 5662-5666 IN denotes from
T15913 5667-5674 NNP denotes Jackson
T15914 5675-5687 NNP denotes Laboratories
T15915 5688-5689 -LRB- denotes (
T15917 5689-5692 NNP denotes Bar
T15916 5693-5699 NNP denotes Harbor
T15918 5699-5701 , denotes ,
T15919 5701-5704 NNP denotes USA
T15920 5704-5705 -RRB- denotes )
T15921 5706-5709 CC denotes and
T15922 5710-5716 NNP denotes Harlan
T15923 5717-5719 NNP denotes UK
T15924 5720-5721 -LRB- denotes (
T15925 5721-5729 NNP denotes Bicester
T15926 5729-5731 , denotes ,
T15927 5731-5733 NNP denotes UK
T15928 5733-5734 -RRB- denotes )
T15929 5734-5736 , denotes ,
T15930 5736-5748 RB denotes respectively
T15931 5748-5749 . denotes .
T15932 5749-5914 sentence denotes All mice were housed in individually ventilated cages in a specific pathogen-free environment with a 12 h light-dark cycle and were fed a regular unrestricted diet.
T15933 5750-5753 DT denotes All
T15934 5754-5758 NNS denotes mice
T15936 5759-5763 VBD denotes were
T15935 5764-5770 VBN denotes housed
T15937 5771-5773 IN denotes in
T15938 5774-5786 RB denotes individually
T15939 5787-5797 VBN denotes ventilated
T15940 5798-5803 NNS denotes cages
T15941 5804-5806 IN denotes in
T15942 5807-5808 DT denotes a
T15944 5809-5817 JJ denotes specific
T15945 5818-5826 NN denotes pathogen
T15947 5826-5827 HYPH denotes -
T15946 5827-5831 JJ denotes free
T15943 5832-5843 NN denotes environment
T15948 5844-5848 IN denotes with
T15949 5849-5850 DT denotes a
T15951 5851-5853 CD denotes 12
T15952 5854-5855 NN denotes h
T15953 5856-5861 JJ denotes light
T15955 5861-5862 HYPH denotes -
T15954 5862-5866 JJ denotes dark
T15950 5867-5872 NN denotes cycle
T15956 5873-5876 CC denotes and
T15957 5877-5881 VBD denotes were
T15958 5882-5885 VBN denotes fed
T15959 5886-5887 DT denotes a
T15961 5888-5895 JJ denotes regular
T15962 5896-5908 JJ denotes unrestricted
T15960 5909-5913 NN denotes diet
T15963 5913-5914 . denotes .
T15964 5914-5986 sentence denotes The GBF's routine surveillance program screened for selected pathogens.
T15965 5915-5918 DT denotes The
T15966 5919-5922 NN denotes GBF
T15968 5922-5924 POS denotes 's
T15969 5925-5932 JJ denotes routine
T15970 5933-5945 NN denotes surveillance
T15967 5946-5953 NN denotes program
T15971 5954-5962 VBD denotes screened
T15972 5963-5966 IN denotes for
T15973 5967-5975 VBN denotes selected
T15974 5976-5985 NNS denotes pathogens
T15975 5985-5986 . denotes .
T15976 5986-6100 sentence denotes The Ptdsrtm1Gbf mutant was crossed to C57BL/6J mice to establish the co-isogenic C57BL/6J-Ptdsrtm1Gbf mouse line.
T15977 5987-5990 DT denotes The
T15979 5991-6002 NN denotes Ptdsrtm1Gbf
T15978 6003-6009 NN denotes mutant
T15981 6010-6013 VBD denotes was
T15980 6014-6021 VBN denotes crossed
T15982 6022-6024 IN denotes to
T15983 6025-6030 NN denotes C57BL
T15985 6030-6031 HYPH denotes /
T15984 6031-6033 NN denotes 6J
T15986 6034-6038 NNS denotes mice
T15987 6039-6041 TO denotes to
T15988 6042-6051 VB denotes establish
T15989 6052-6055 DT denotes the
T15991 6056-6067 JJ denotes co-isogenic
T15992 6068-6073 NN denotes C57BL
T15994 6073-6074 HYPH denotes /
T15993 6074-6076 NN denotes 6J
T15996 6076-6077 HYPH denotes -
T15995 6077-6088 NN denotes Ptdsrtm1Gbf
T15997 6089-6094 NN denotes mouse
T15990 6095-6099 NN denotes line
T15998 6099-6100 . denotes .
T15999 6100-6158 sentence denotes All studies were approved by the appropriate authorities.
T16000 6101-6104 DT denotes All
T16001 6105-6112 NNS denotes studies
T16003 6113-6117 VBD denotes were
T16002 6118-6126 VBN denotes approved
T16004 6127-6129 IN denotes by
T16005 6130-6133 DT denotes the
T16007 6134-6145 JJ denotes appropriate
T16006 6146-6157 NNS denotes authorities
T16008 6157-6158 . denotes .
T16039 6160-6169 NN denotes Isolation
T16040 6170-6172 IN denotes of
T16041 6173-6180 NNS denotes embryos
T16042 6180-6276 sentence denotes Heterozygous male and female mice were intercrossed in order to obtain Ptdsr-deficient progeny.
T16043 6181-6193 JJ denotes Heterozygous
T16045 6194-6198 JJ denotes male
T16046 6199-6202 CC denotes and
T16047 6203-6209 JJ denotes female
T16044 6210-6214 NNS denotes mice
T16049 6215-6219 VBD denotes were
T16048 6220-6232 VBN denotes intercrossed
T16050 6233-6235 IN denotes in
T16051 6236-6241 NN denotes order
T16052 6242-6244 TO denotes to
T16053 6245-6251 VB denotes obtain
T16054 6252-6257 NN denotes Ptdsr
T16056 6257-6258 HYPH denotes -
T16055 6258-6267 JJ denotes deficient
T16057 6268-6275 NN denotes progeny
T16058 6275-6276 . denotes .
T16059 6276-6383 sentence denotes Females were daily monitored for vaginal plugs, and noon of the day of plug detection was defined as E0.5.
T16060 6277-6284 NNS denotes Females
T16062 6285-6289 VBD denotes were
T16063 6290-6295 RB denotes daily
T16061 6296-6305 VBN denotes monitored
T16064 6306-6309 IN denotes for
T16065 6310-6317 JJ denotes vaginal
T16066 6318-6323 NNS denotes plugs
T16067 6323-6325 , denotes ,
T16068 6325-6328 CC denotes and
T16069 6329-6333 NN denotes noon
T16071 6334-6336 IN denotes of
T16072 6337-6340 DT denotes the
T16073 6341-6344 NN denotes day
T16074 6345-6347 IN denotes of
T16075 6348-6352 NN denotes plug
T16076 6353-6362 NN denotes detection
T16077 6363-6366 VBD denotes was
T16070 6367-6374 VBN denotes defined
T16078 6375-6377 IN denotes as
T16079 6378-6382 NN denotes E0.5
T16080 6382-6383 . denotes .
T16081 6383-6504 sentence denotes Embryos at indicated time points were dissected in sterile PBS, washed in ice-cold PBS and transferred to cold fixative.
T16082 6384-6391 NNS denotes Embryos
T16084 6392-6394 IN denotes at
T16085 6395-6404 VBN denotes indicated
T16087 6405-6409 NN denotes time
T16086 6410-6416 NNS denotes points
T16088 6417-6421 VBD denotes were
T16083 6422-6431 VBN denotes dissected
T16089 6432-6434 IN denotes in
T16090 6435-6442 JJ denotes sterile
T16091 6443-6446 NN denotes PBS
T16092 6446-6448 , denotes ,
T16093 6448-6454 VBN denotes washed
T16094 6455-6457 IN denotes in
T16095 6458-6461 NN denotes ice
T16097 6461-6462 HYPH denotes -
T16096 6462-6466 JJ denotes cold
T16098 6467-6470 NN denotes PBS
T16099 6471-6474 CC denotes and
T16100 6475-6486 VBN denotes transferred
T16101 6487-6489 IN denotes to
T16102 6490-6494 JJ denotes cold
T16103 6495-6503 NN denotes fixative
T16104 6503-6504 . denotes .
T16105 6504-6565 sentence denotes Extra-embryonic membranes were kept and used for genotyping.
T16106 6505-6520 JJ denotes Extra-embryonic
T16107 6521-6530 NNS denotes membranes
T16109 6531-6535 VBD denotes were
T16108 6536-6540 VBN denotes kept
T16110 6541-6544 CC denotes and
T16111 6545-6549 VBN denotes used
T16112 6550-6553 IN denotes for
T16113 6554-6564 NN denotes genotyping
T16114 6564-6565 . denotes .
T16115 6565-6642 sentence denotes Ptdsr -/- embryos and their wild-type littermates were used for experiments.
T16116 6566-6571 NN denotes Ptdsr
T16118 6572-6573 SYM denotes -
T16119 6573-6574 HYPH denotes /
T16120 6574-6575 SYM denotes -
T16117 6576-6583 NNS denotes embryos
T16122 6584-6587 CC denotes and
T16123 6588-6593 PRP$ denotes their
T16125 6594-6598 JJ denotes wild
T16127 6598-6599 HYPH denotes -
T16126 6599-6603 NN denotes type
T16124 6604-6615 NNS denotes littermates
T16128 6616-6620 VBD denotes were
T16121 6621-6625 VBN denotes used
T16129 6626-6629 IN denotes for
T16130 6630-6641 NNS denotes experiments
T16131 6641-6642 . denotes .
T16203 6644-6653 NN denotes Histology
T16204 6653-6655 , denotes ,
T16205 6655-6660 NN denotes TUNEL
T16206 6661-6669 NN denotes staining
T16207 6670-6673 CC denotes and
T16208 6674-6694 NN denotes immunohistochemistry
T16209 6694-7021 sentence denotes Embryos for histology and immunohistochemistry were harvested and fixed in 10% neutral-buffered formalin, dehydrated through a graded series of alcohol, embedded in paraffin, sagittally sectioned at 5 μm intervals, and every fifth section was processed for hematoxylin and eosin (H&E) staining according to standard protocols.
T16210 6695-6702 NNS denotes Embryos
T16212 6703-6706 IN denotes for
T16213 6707-6716 NN denotes histology
T16214 6717-6720 CC denotes and
T16215 6721-6741 NN denotes immunohistochemistry
T16216 6742-6746 VBD denotes were
T16211 6747-6756 VBN denotes harvested
T16217 6757-6760 CC denotes and
T16218 6761-6766 VBN denotes fixed
T16219 6767-6769 IN denotes in
T16220 6770-6772 CD denotes 10
T16221 6772-6773 NN denotes %
T16223 6774-6781 RB denotes neutral
T16225 6781-6782 HYPH denotes -
T16224 6782-6790 VBN denotes buffered
T16222 6791-6799 NN denotes formalin
T16226 6799-6801 , denotes ,
T16227 6801-6811 VBN denotes dehydrated
T16228 6812-6819 IN denotes through
T16229 6820-6821 DT denotes a
T16231 6822-6828 JJ denotes graded
T16230 6829-6835 NN denotes series
T16232 6836-6838 IN denotes of
T16233 6839-6846 NN denotes alcohol
T16234 6846-6848 , denotes ,
T16235 6848-6856 VBN denotes embedded
T16236 6857-6859 IN denotes in
T16237 6860-6868 NN denotes paraffin
T16238 6868-6870 , denotes ,
T16239 6870-6880 RB denotes sagittally
T16240 6881-6890 VBN denotes sectioned
T16241 6891-6893 IN denotes at
T16242 6894-6895 CD denotes 5
T16243 6896-6898 NN denotes μm
T16244 6899-6908 NNS denotes intervals
T16245 6908-6910 , denotes ,
T16246 6910-6913 CC denotes and
T16247 6914-6919 DT denotes every
T16249 6920-6925 JJ denotes fifth
T16248 6926-6933 NN denotes section
T16251 6934-6937 VBD denotes was
T16250 6938-6947 VBN denotes processed
T16252 6948-6951 IN denotes for
T16253 6952-6963 NN denotes hematoxylin
T16255 6964-6967 CC denotes and
T16256 6968-6973 NN denotes eosin
T16257 6974-6975 -LRB- denotes (
T16258 6975-6976 NN denotes H
T16259 6976-6977 CC denotes &
T16260 6977-6978 NN denotes E
T16261 6978-6979 -RRB- denotes )
T16254 6980-6988 NN denotes staining
T16262 6989-6998 VBG denotes according
T16263 6999-7001 IN denotes to
T16264 7002-7010 JJ denotes standard
T16265 7011-7020 NNS denotes protocols
T16266 7020-7021 . denotes .
T16267 7021-7113 sentence denotes Remaining sections of wild-type and Ptdsr -/- specimens were used for immunohistochemistry.
T16268 7022-7031 VBG denotes Remaining
T16269 7032-7040 NNS denotes sections
T16271 7041-7043 IN denotes of
T16272 7044-7048 JJ denotes wild
T16274 7048-7049 HYPH denotes -
T16273 7049-7053 NN denotes type
T16276 7054-7057 CC denotes and
T16277 7058-7063 NN denotes Ptdsr
T16278 7064-7065 SYM denotes -
T16279 7065-7066 HYPH denotes /
T16280 7066-7067 SYM denotes -
T16275 7068-7077 NNS denotes specimens
T16281 7078-7082 VBD denotes were
T16270 7083-7087 VBN denotes used
T16282 7088-7091 IN denotes for
T16283 7092-7112 NN denotes immunohistochemistry
T16284 7112-7113 . denotes .
T16285 7113-7366 sentence denotes For detection of apoptotic cells and macrophages, anti-aCasp3 (an antibody specific for activated caspase 3; R&D Systems, Minneapolis, USA) and anti-F4/80 (Serotec GmBH, Düsseldorf, Germany; #MCA 1957) antibodies were used as described by the supplier.
T16286 7114-7117 IN denotes For
T16288 7118-7127 NN denotes detection
T16289 7128-7130 IN denotes of
T16290 7131-7140 JJ denotes apoptotic
T16291 7141-7146 NNS denotes cells
T16292 7147-7150 CC denotes and
T16293 7151-7162 NNS denotes macrophages
T16294 7162-7164 , denotes ,
T16295 7164-7175 NN denotes anti-aCasp3
T16296 7176-7177 -LRB- denotes (
T16298 7177-7179 DT denotes an
T16299 7180-7188 NN denotes antibody
T16300 7189-7197 JJ denotes specific
T16301 7198-7201 IN denotes for
T16302 7202-7211 VBN denotes activated
T16303 7212-7219 NN denotes caspase
T16304 7220-7221 CD denotes 3
T16305 7221-7222 : denotes ;
T16306 7223-7224 NNP denotes R
T16307 7224-7225 CC denotes &
T16308 7225-7226 NNP denotes D
T16297 7227-7234 NNP denotes Systems
T16309 7234-7236 , denotes ,
T16310 7236-7247 NNP denotes Minneapolis
T16311 7247-7249 , denotes ,
T16312 7249-7252 NNP denotes USA
T16313 7252-7253 -RRB- denotes )
T16314 7254-7257 CC denotes and
T16315 7258-7265 NN denotes anti-F4
T16317 7265-7266 HYPH denotes /
T16318 7266-7268 CD denotes 80
T16319 7269-7270 -LRB- denotes (
T16321 7270-7277 NNP denotes Serotec
T16322 7278-7282 NNP denotes GmBH
T16323 7282-7284 , denotes ,
T16324 7284-7294 NNP denotes Düsseldorf
T16325 7294-7296 , denotes ,
T16326 7296-7303 NNP denotes Germany
T16327 7303-7304 : denotes ;
T16328 7305-7306 NN denotes #
T16320 7306-7309 NNP denotes MCA
T16329 7310-7314 CD denotes 1957
T16330 7314-7315 -RRB- denotes )
T16316 7316-7326 NNS denotes antibodies
T16331 7327-7331 VBD denotes were
T16287 7332-7336 VBN denotes used
T16332 7337-7339 IN denotes as
T16333 7340-7349 VBN denotes described
T16334 7350-7352 IN denotes by
T16335 7353-7356 DT denotes the
T16336 7357-7365 NN denotes supplier
T16337 7365-7366 . denotes .
T16338 7366-7535 sentence denotes Detection was performed using indirect streptavidin with biotinylated secondary antibodies and cobalt-enhanced diaminobenzidine (brown) or fast-red (red) as chromogens.
T16339 7367-7376 NN denotes Detection
T16341 7377-7380 VBD denotes was
T16340 7381-7390 VBN denotes performed
T16342 7391-7396 VBG denotes using
T16343 7397-7405 JJ denotes indirect
T16344 7406-7418 NN denotes streptavidin
T16345 7419-7423 IN denotes with
T16346 7424-7436 VBN denotes biotinylated
T16348 7437-7446 JJ denotes secondary
T16347 7447-7457 NNS denotes antibodies
T16349 7458-7461 CC denotes and
T16350 7462-7468 NN denotes cobalt
T16352 7468-7469 HYPH denotes -
T16351 7469-7477 VBN denotes enhanced
T16353 7478-7494 NN denotes diaminobenzidine
T16354 7495-7496 -LRB- denotes (
T16355 7496-7501 JJ denotes brown
T16356 7501-7502 -RRB- denotes )
T16357 7503-7505 CC denotes or
T16358 7506-7510 JJ denotes fast
T16360 7510-7511 HYPH denotes -
T16359 7511-7514 NN denotes red
T16361 7515-7516 -LRB- denotes (
T16362 7516-7519 JJ denotes red
T16363 7519-7520 -RRB- denotes )
T16364 7521-7523 IN denotes as
T16365 7524-7534 NNS denotes chromogens
T16366 7534-7535 . denotes .
T16367 7535-7582 sentence denotes Sections were counterstained with hematoxylin.
T16368 7536-7544 NNS denotes Sections
T16370 7545-7549 VBD denotes were
T16369 7550-7564 VBN denotes counterstained
T16371 7565-7569 IN denotes with
T16372 7570-7581 NN denotes hematoxylin
T16373 7581-7582 . denotes .
T16374 7582-7812 sentence denotes For whole-mount terminal deoxynucleotidyl transferase-mediated UTP end labeling (TUNEL), limb buds were dissected from E12.5 and E13.5 embryos, fixed in 4% paraformaldehyde and processed for analysis as previously described [50].
T16375 7583-7586 IN denotes For
T16377 7587-7592 JJ denotes whole
T16379 7592-7593 HYPH denotes -
T16378 7593-7598 NN denotes mount
T16381 7599-7607 JJ denotes terminal
T16382 7608-7624 NN denotes deoxynucleotidyl
T16383 7625-7636 NN denotes transferase
T16385 7636-7637 HYPH denotes -
T16384 7637-7645 VBN denotes mediated
T16386 7646-7649 NN denotes UTP
T16387 7650-7653 NN denotes end
T16380 7654-7662 NN denotes labeling
T16388 7663-7664 -LRB- denotes (
T16389 7664-7669 NN denotes TUNEL
T16390 7669-7670 -RRB- denotes )
T16391 7670-7672 , denotes ,
T16392 7672-7676 NN denotes limb
T16393 7677-7681 NNS denotes buds
T16394 7682-7686 VBD denotes were
T16376 7687-7696 VBN denotes dissected
T16395 7697-7701 IN denotes from
T16396 7702-7707 NN denotes E12.5
T16398 7708-7711 CC denotes and
T16399 7712-7717 NN denotes E13.5
T16397 7718-7725 NNS denotes embryos
T16400 7725-7727 , denotes ,
T16401 7727-7732 VBN denotes fixed
T16402 7733-7735 IN denotes in
T16403 7736-7737 CD denotes 4
T16404 7737-7738 NN denotes %
T16405 7739-7755 NN denotes paraformaldehyde
T16406 7756-7759 CC denotes and
T16407 7760-7769 VBN denotes processed
T16408 7770-7773 IN denotes for
T16409 7774-7782 NN denotes analysis
T16410 7783-7785 IN denotes as
T16412 7786-7796 RB denotes previously
T16411 7797-7806 VBN denotes described
T16413 7807-7808 -LRB- denotes [
T16414 7808-7810 CD denotes 50
T16415 7810-7811 -RRB- denotes ]
T16416 7811-7812 . denotes .
T16464 7814-7825 NN denotes Preparation
T16465 7826-7828 IN denotes of
T16466 7829-7834 JJ denotes fetal
T16467 7835-7840 NN denotes liver
T16469 7840-7841 HYPH denotes -
T16468 7841-7848 VBN denotes derived
T16470 7849-7860 NNS denotes macrophages
T16471 7861-7862 -LRB- denotes (
T16472 7862-7867 NNS denotes FLDMs
T16473 7867-7868 -RRB- denotes )
T16474 7868-8005 sentence denotes Fetal livers were excised from embryos at E12.5 and E13.5, respectively, washed in PBS and dissociated enzymatically for 60 min at 37°C.
T16475 7869-7874 JJ denotes Fetal
T16476 7875-7881 NNS denotes livers
T16478 7882-7886 VBD denotes were
T16477 7887-7894 VBN denotes excised
T16479 7895-7899 IN denotes from
T16480 7900-7907 NNS denotes embryos
T16481 7908-7910 IN denotes at
T16482 7911-7916 NN denotes E12.5
T16483 7917-7920 CC denotes and
T16484 7921-7926 NN denotes E13.5
T16485 7926-7928 , denotes ,
T16486 7928-7940 RB denotes respectively
T16487 7940-7942 , denotes ,
T16488 7942-7948 VBN denotes washed
T16489 7949-7951 IN denotes in
T16490 7952-7955 NN denotes PBS
T16491 7956-7959 CC denotes and
T16492 7960-7971 VBN denotes dissociated
T16493 7972-7985 RB denotes enzymatically
T16494 7986-7989 IN denotes for
T16495 7990-7992 CD denotes 60
T16496 7993-7996 NNS denotes min
T16497 7997-7999 IN denotes at
T16498 8000-8002 CD denotes 37
T16499 8002-8004 NN denotes °C
T16500 8004-8005 . denotes .
T16501 8005-8151 sentence denotes The digestion buffer (150 μl per liver) comprised 0.6 U/ml dispase I (Roche), 0.1% collagenase D (Roche), 10 U DNase (Roche), and 20% FCS in PBS.
T16502 8006-8009 DT denotes The
T16504 8010-8019 NN denotes digestion
T16503 8020-8026 NN denotes buffer
T16506 8027-8028 -LRB- denotes (
T16508 8028-8031 CD denotes 150
T16507 8032-8034 NNS denotes μl
T16509 8035-8038 IN denotes per
T16510 8039-8044 NN denotes liver
T16511 8044-8045 -RRB- denotes )
T16505 8046-8055 VBD denotes comprised
T16512 8056-8059 CD denotes 0.6
T16513 8060-8061 NN denotes U
T16515 8061-8062 SYM denotes /
T16516 8062-8064 NN denotes ml
T16514 8065-8072 NN denotes dispase
T16517 8073-8074 CD denotes I
T16518 8075-8076 -LRB- denotes (
T16519 8076-8081 NNP denotes Roche
T16520 8081-8082 -RRB- denotes )
T16521 8082-8084 , denotes ,
T16522 8084-8087 CD denotes 0.1
T16523 8087-8088 NN denotes %
T16525 8089-8100 NN denotes collagenase
T16524 8101-8102 NN denotes D
T16526 8103-8104 -LRB- denotes (
T16527 8104-8109 NNP denotes Roche
T16528 8109-8110 -RRB- denotes )
T16529 8110-8112 , denotes ,
T16530 8112-8114 CD denotes 10
T16531 8115-8116 NN denotes U
T16532 8117-8122 NN denotes DNase
T16533 8123-8124 -LRB- denotes (
T16534 8124-8129 NNP denotes Roche
T16535 8129-8130 -RRB- denotes )
T16536 8130-8132 , denotes ,
T16537 8132-8135 CC denotes and
T16538 8136-8138 CD denotes 20
T16539 8138-8139 NN denotes %
T16540 8140-8143 NN denotes FCS
T16541 8144-8146 IN denotes in
T16542 8147-8150 NN denotes PBS
T16543 8150-8151 . denotes .
T16544 8151-8503 sentence denotes X-Vivo 15 medium (Cambrex, East Rutherford, USA) was added to the resulting cell suspension, and after centrifugation (200 × g; 3 min) cells were resuspended in X-Vivo 15 medium supplemented with 50 ng/ml macrophage colony-stimulating factor (M-CSF; Sigma-Aldrich, St. Louis, USA) and cultured on non-treated tissue-culture dishes at 37°C with 5% CO2.
T16545 8152-8153 NN denotes X
T16547 8153-8154 HYPH denotes -
T16548 8154-8158 NN denotes Vivo
T16549 8159-8161 CD denotes 15
T16546 8162-8168 NN denotes medium
T16551 8169-8170 -LRB- denotes (
T16552 8170-8177 NNP denotes Cambrex
T16553 8177-8179 , denotes ,
T16554 8179-8183 NNP denotes East
T16555 8184-8194 NNP denotes Rutherford
T16556 8194-8196 , denotes ,
T16557 8196-8199 NNP denotes USA
T16558 8199-8200 -RRB- denotes )
T16559 8201-8204 VBD denotes was
T16550 8205-8210 VBN denotes added
T16560 8211-8213 IN denotes to
T16561 8214-8217 DT denotes the
T16563 8218-8227 VBG denotes resulting
T16564 8228-8232 NN denotes cell
T16562 8233-8243 NN denotes suspension
T16565 8243-8245 , denotes ,
T16566 8245-8248 CC denotes and
T16567 8249-8254 IN denotes after
T16569 8255-8269 NN denotes centrifugation
T16570 8270-8271 -LRB- denotes (
T16572 8271-8274 CD denotes 200
T16573 8275-8276 SYM denotes ×
T16571 8277-8278 NN denotes g
T16574 8278-8279 : denotes ;
T16575 8280-8281 CD denotes 3
T16576 8282-8285 NNS denotes min
T16577 8285-8286 -RRB- denotes )
T16578 8287-8292 NNS denotes cells
T16579 8293-8297 VBD denotes were
T16568 8298-8309 VBN denotes resuspended
T16580 8310-8312 IN denotes in
T16581 8313-8314 NN denotes X
T16583 8314-8315 HYPH denotes -
T16582 8315-8319 NN denotes Vivo
T16585 8320-8322 CD denotes 15
T16584 8323-8329 NN denotes medium
T16586 8330-8342 VBN denotes supplemented
T16587 8343-8347 IN denotes with
T16588 8348-8350 CD denotes 50
T16589 8351-8353 NN denotes ng
T16591 8353-8354 SYM denotes /
T16592 8354-8356 NN denotes ml
T16593 8357-8367 NN denotes macrophage
T16594 8368-8374 NN denotes colony
T16596 8374-8375 HYPH denotes -
T16595 8375-8386 VBG denotes stimulating
T16590 8387-8393 NN denotes factor
T16597 8394-8395 -LRB- denotes (
T16599 8395-8396 NN denotes M
T16601 8396-8397 HYPH denotes -
T16600 8397-8400 NN denotes CSF
T16602 8400-8401 : denotes ;
T16603 8402-8407 NNP denotes Sigma
T16604 8407-8408 HYPH denotes -
T16598 8408-8415 NNP denotes Aldrich
T16605 8415-8417 , denotes ,
T16606 8417-8420 NNP denotes St.
T16607 8421-8426 NNP denotes Louis
T16608 8426-8428 , denotes ,
T16609 8428-8431 NNP denotes USA
T16610 8431-8432 -RRB- denotes )
T16611 8433-8436 CC denotes and
T16612 8437-8445 VBN denotes cultured
T16613 8446-8448 IN denotes on
T16614 8449-8460 JJ denotes non-treated
T16616 8461-8467 NN denotes tissue
T16617 8467-8468 HYPH denotes -
T16615 8468-8475 NN denotes culture
T16618 8476-8482 NNS denotes dishes
T16619 8483-8485 IN denotes at
T16620 8486-8488 CD denotes 37
T16621 8488-8490 NN denotes °C
T16622 8491-8495 IN denotes with
T16623 8496-8497 CD denotes 5
T16624 8497-8498 NN denotes %
T16625 8499-8502 NN denotes CO2
T16626 8502-8503 . denotes .
T16627 8503-8571 sentence denotes Every second or third day the medium was changed by centrifugation.
T16628 8504-8509 DT denotes Every
T16630 8510-8516 JJ denotes second
T16631 8517-8519 CC denotes or
T16632 8520-8525 JJ denotes third
T16629 8526-8529 NN denotes day
T16634 8530-8533 DT denotes the
T16635 8534-8540 NN denotes medium
T16636 8541-8544 VBD denotes was
T16633 8545-8552 VBN denotes changed
T16637 8553-8555 IN denotes by
T16638 8556-8570 NN denotes centrifugation
T16639 8570-8571 . denotes .
T16640 8571-8702 sentence denotes Following withdrawal of M-CSF on day 6 after excision, adherent cells were cultured for an additional 24-48 h in X-Vivo 15 medium.
T16641 8572-8581 IN denotes Following
T16643 8582-8592 NN denotes withdrawal
T16644 8593-8595 IN denotes of
T16645 8596-8597 NN denotes M
T16647 8597-8598 HYPH denotes -
T16646 8598-8601 NN denotes CSF
T16648 8602-8604 IN denotes on
T16649 8605-8608 NN denotes day
T16650 8609-8610 CD denotes 6
T16651 8611-8616 IN denotes after
T16652 8617-8625 NN denotes excision
T16653 8625-8627 , denotes ,
T16654 8627-8635 JJ denotes adherent
T16655 8636-8641 NNS denotes cells
T16656 8642-8646 VBD denotes were
T16642 8647-8655 VBN denotes cultured
T16657 8656-8659 IN denotes for
T16658 8660-8662 DT denotes an
T16660 8663-8673 JJ denotes additional
T16661 8674-8676 CD denotes 24
T16663 8676-8677 HYPH denotes -
T16662 8677-8679 CD denotes 48
T16659 8680-8681 NNS denotes h
T16664 8682-8684 IN denotes in
T16665 8685-8686 NN denotes X
T16667 8686-8687 HYPH denotes -
T16666 8687-8691 NN denotes Vivo
T16669 8692-8694 CD denotes 15
T16668 8695-8701 NN denotes medium
T16670 8701-8702 . denotes .
T16840 8704-8714 NN denotes Macrophage
T16842 8715-8727 NN denotes phagocytosis
T16841 8728-8734 NNS denotes assays
T16843 8734-8890 sentence denotes For preparation of monolayer cultures of macrophages, FLDMs were plated on glass coverslips in 24 well plates (2 × 105 cells per well) in X-Vivo 15 medium.
T16844 8735-8738 IN denotes For
T16846 8739-8750 NN denotes preparation
T16847 8751-8753 IN denotes of
T16848 8754-8763 NN denotes monolayer
T16849 8764-8772 NNS denotes cultures
T16850 8773-8775 IN denotes of
T16851 8776-8787 NNS denotes macrophages
T16852 8787-8789 , denotes ,
T16853 8789-8794 NNS denotes FLDMs
T16854 8795-8799 VBD denotes were
T16845 8800-8806 VBN denotes plated
T16855 8807-8809 IN denotes on
T16856 8810-8815 NN denotes glass
T16857 8816-8826 NNS denotes coverslips
T16858 8827-8829 IN denotes in
T16859 8830-8832 CD denotes 24
T16861 8833-8837 NN denotes well
T16860 8838-8844 NNS denotes plates
T16862 8845-8846 -LRB- denotes (
T16864 8846-8847 CD denotes 2
T16866 8848-8849 SYM denotes ×
T16865 8850-8853 CD denotes 105
T16863 8854-8859 NNS denotes cells
T16867 8860-8863 IN denotes per
T16868 8864-8868 NN denotes well
T16869 8868-8869 -RRB- denotes )
T16870 8870-8872 IN denotes in
T16871 8873-8874 NN denotes X
T16873 8874-8875 HYPH denotes -
T16872 8875-8879 NN denotes Vivo
T16875 8880-8882 CD denotes 15
T16874 8883-8889 NN denotes medium
T16876 8889-8890 . denotes .
T16877 8890-9193 sentence denotes For preparation of apoptotic target cells, primary thymocytes were harvested from the thymus of 4- to 8-week-old C57BL/6J mice, stained with TAMRA for 15 min, and apoptosis was induced either by treating cells with 5 μM staurosporine in medium for 4 h at 37°C or by culturing cells in medium overnight.
T16878 8891-8894 IN denotes For
T16880 8895-8906 NN denotes preparation
T16881 8907-8909 IN denotes of
T16882 8910-8919 JJ denotes apoptotic
T16884 8920-8926 NN denotes target
T16883 8927-8932 NNS denotes cells
T16885 8932-8934 , denotes ,
T16886 8934-8941 JJ denotes primary
T16887 8942-8952 NNS denotes thymocytes
T16888 8953-8957 VBD denotes were
T16879 8958-8967 VBN denotes harvested
T16889 8968-8972 IN denotes from
T16890 8973-8976 DT denotes the
T16891 8977-8983 NN denotes thymus
T16892 8984-8986 IN denotes of
T16893 8987-8988 CD denotes 4
T16895 8988-8989 HYPH denotes -
T16896 8990-8992 IN denotes to
T16894 8993-8994 CD denotes 8
T16898 8994-8995 HYPH denotes -
T16897 8995-8999 NN denotes week
T16900 8999-9000 HYPH denotes -
T16899 9000-9003 JJ denotes old
T16902 9004-9009 NN denotes C57BL
T16904 9009-9010 HYPH denotes /
T16903 9010-9012 NN denotes 6J
T16901 9013-9017 NNS denotes mice
T16905 9017-9019 , denotes ,
T16906 9019-9026 VBN denotes stained
T16907 9027-9031 IN denotes with
T16908 9032-9037 NN denotes TAMRA
T16909 9038-9041 IN denotes for
T16910 9042-9044 CD denotes 15
T16911 9045-9048 NNS denotes min
T16912 9048-9050 , denotes ,
T16913 9050-9053 CC denotes and
T16914 9054-9063 NN denotes apoptosis
T16916 9064-9067 VBD denotes was
T16915 9068-9075 VBN denotes induced
T16917 9076-9082 CC denotes either
T16918 9083-9085 IN denotes by
T16919 9086-9094 VBG denotes treating
T16920 9095-9100 NNS denotes cells
T16921 9101-9105 IN denotes with
T16922 9106-9107 CD denotes 5
T16923 9108-9110 NN denotes μM
T16924 9111-9124 NN denotes staurosporine
T16925 9125-9127 IN denotes in
T16926 9128-9134 NN denotes medium
T16927 9135-9138 IN denotes for
T16928 9139-9140 CD denotes 4
T16929 9141-9142 NNS denotes h
T16930 9143-9145 IN denotes at
T16931 9146-9148 CD denotes 37
T16932 9148-9150 NN denotes °C
T16933 9151-9153 CC denotes or
T16934 9154-9156 IN denotes by
T16935 9157-9166 VBG denotes culturing
T16936 9167-9172 NNS denotes cells
T16937 9173-9175 IN denotes in
T16938 9176-9182 NN denotes medium
T16939 9183-9192 RB denotes overnight
T16940 9192-9193 . denotes .
T16941 9193-9296 sentence denotes The efficacy of apoptosis induction was compared in thymic target cells and controls by FACS analysis.
T16942 9194-9197 DT denotes The
T16943 9198-9206 NN denotes efficacy
T16945 9207-9209 IN denotes of
T16946 9210-9219 NN denotes apoptosis
T16947 9220-9229 NN denotes induction
T16948 9230-9233 VBD denotes was
T16944 9234-9242 VBN denotes compared
T16949 9243-9245 IN denotes in
T16950 9246-9252 JJ denotes thymic
T16952 9253-9259 NN denotes target
T16951 9260-9265 NNS denotes cells
T16953 9266-9269 CC denotes and
T16954 9270-9278 NNS denotes controls
T16955 9279-9281 IN denotes by
T16956 9282-9286 NN denotes FACS
T16957 9287-9295 NN denotes analysis
T16958 9295-9296 . denotes .
T16959 9296-9510 sentence denotes On average, 60% of the cells of the resulting population were apoptotic, with exposed PS on their surface, and less than 5% of the cells were necrotic, as confirmed by FITC-annexin V and propidium iodide staining.
T16960 9297-9299 IN denotes On
T16962 9300-9307 JJ denotes average
T16963 9307-9309 , denotes ,
T16964 9309-9311 CD denotes 60
T16965 9311-9312 NN denotes %
T16966 9313-9315 IN denotes of
T16967 9316-9319 DT denotes the
T16968 9320-9325 NNS denotes cells
T16969 9326-9328 IN denotes of
T16970 9329-9332 DT denotes the
T16972 9333-9342 VBG denotes resulting
T16971 9343-9353 NN denotes population
T16961 9354-9358 VBD denotes were
T16973 9359-9368 JJ denotes apoptotic
T16974 9368-9370 , denotes ,
T16975 9370-9374 IN denotes with
T16976 9375-9382 VBN denotes exposed
T16977 9383-9385 NN denotes PS
T16978 9386-9388 IN denotes on
T16979 9389-9394 PRP$ denotes their
T16980 9395-9402 NN denotes surface
T16981 9402-9404 , denotes ,
T16982 9404-9407 CC denotes and
T16983 9408-9412 JJR denotes less
T16985 9413-9417 IN denotes than
T16984 9418-9419 CD denotes 5
T16986 9419-9420 NN denotes %
T16988 9421-9423 IN denotes of
T16989 9424-9427 DT denotes the
T16990 9428-9433 NNS denotes cells
T16987 9434-9438 VBD denotes were
T16991 9439-9447 JJ denotes necrotic
T16992 9447-9449 , denotes ,
T16993 9449-9451 IN denotes as
T16994 9452-9461 VBN denotes confirmed
T16995 9462-9464 IN denotes by
T16996 9465-9469 NN denotes FITC
T16998 9469-9470 HYPH denotes -
T16997 9470-9477 NN denotes annexin
T16999 9478-9479 CD denotes V
T17000 9480-9483 CC denotes and
T17001 9484-9493 NN denotes propidium
T17002 9494-9500 NN denotes iodide
T17003 9501-9509 NN denotes staining
T17004 9509-9510 . denotes .
T17005 9510-9619 sentence denotes The apoptotic thymocytes obtained were washed with PBS and added to the prepared FLDM cultures (ratio 10:1).
T17006 9511-9514 DT denotes The
T17008 9515-9524 JJ denotes apoptotic
T17007 9525-9535 NNS denotes thymocytes
T17010 9536-9544 VBN denotes obtained
T17011 9545-9549 VBD denotes were
T17009 9550-9556 VBN denotes washed
T17012 9557-9561 IN denotes with
T17013 9562-9565 NN denotes PBS
T17014 9566-9569 CC denotes and
T17015 9570-9575 VBN denotes added
T17016 9576-9578 IN denotes to
T17017 9579-9582 DT denotes the
T17019 9583-9591 VBN denotes prepared
T17020 9592-9596 NN denotes FLDM
T17018 9597-9605 NNS denotes cultures
T17021 9606-9607 -LRB- denotes (
T17023 9607-9612 NN denotes ratio
T17022 9613-9615 CD denotes 10
T17024 9615-9616 SYM denotes :
T17025 9616-9617 CD denotes 1
T17026 9617-9618 -RRB- denotes )
T17027 9618-9619 . denotes .
T17028 9619-9680 sentence denotes Phagocytosis was then allowed to proceed at 37°C and 5% CO2.
T17029 9620-9632 NN denotes Phagocytosis
T17031 9633-9636 VBD denotes was
T17032 9637-9641 RB denotes then
T17030 9642-9649 VBN denotes allowed
T17033 9650-9652 TO denotes to
T17034 9653-9660 VB denotes proceed
T17035 9661-9663 IN denotes at
T17036 9664-9666 CD denotes 37
T17037 9666-9668 NN denotes °C
T17038 9669-9672 CC denotes and
T17039 9673-9674 CD denotes 5
T17040 9674-9675 NN denotes %
T17041 9676-9679 NN denotes CO2
T17042 9679-9680 . denotes .
T17043 9680-9847 sentence denotes After the indicated time periods, the uptake of apoptotic cells by FLDMs was stopped by intensive washing of co-cultures with cold PBS to remove unphagocytosed cells.
T17044 9681-9686 IN denotes After
T17046 9687-9690 DT denotes the
T17048 9691-9700 VBN denotes indicated
T17049 9701-9705 NN denotes time
T17047 9706-9713 NNS denotes periods
T17050 9713-9715 , denotes ,
T17051 9715-9718 DT denotes the
T17052 9719-9725 NN denotes uptake
T17053 9726-9728 IN denotes of
T17054 9729-9738 JJ denotes apoptotic
T17055 9739-9744 NNS denotes cells
T17056 9745-9747 IN denotes by
T17057 9748-9753 NNS denotes FLDMs
T17058 9754-9757 VBD denotes was
T17045 9758-9765 VBN denotes stopped
T17059 9766-9768 IN denotes by
T17060 9769-9778 JJ denotes intensive
T17061 9779-9786 NN denotes washing
T17062 9787-9789 IN denotes of
T17063 9790-9801 NNS denotes co-cultures
T17064 9802-9806 IN denotes with
T17065 9807-9811 JJ denotes cold
T17066 9812-9815 NN denotes PBS
T17067 9816-9818 TO denotes to
T17068 9819-9825 VB denotes remove
T17069 9826-9840 JJ denotes unphagocytosed
T17070 9841-9846 NNS denotes cells
T17071 9846-9847 . denotes .
T17072 9847-9964 sentence denotes To measure phagocytosis of apoptotic thymocytes, macrophages were further processed for immunofluorescence analysis.
T17073 9848-9850 TO denotes To
T17074 9851-9858 VB denotes measure
T17076 9859-9871 NN denotes phagocytosis
T17077 9872-9874 IN denotes of
T17078 9875-9884 JJ denotes apoptotic
T17079 9885-9895 NNS denotes thymocytes
T17080 9895-9897 , denotes ,
T17081 9897-9908 NNS denotes macrophages
T17082 9909-9913 VBD denotes were
T17083 9914-9921 RB denotes further
T17075 9922-9931 VBN denotes processed
T17084 9932-9935 IN denotes for
T17085 9936-9954 NN denotes immunofluorescence
T17086 9955-9963 NN denotes analysis
T17087 9963-9964 . denotes .
T17088 9964-10171 sentence denotes Cells were fixed in 4% paraformaldehyde, blocked in 0.5% BSA/PBS and stained with an anti-F4/80 antibody (Serotec) followed by a secondary antibody coupled to Alexa 488 (Molecular Probes Inc., Eugene, USA).
T17089 9965-9970 NNS denotes Cells
T17091 9971-9975 VBD denotes were
T17090 9976-9981 VBN denotes fixed
T17092 9982-9984 IN denotes in
T17093 9985-9986 CD denotes 4
T17094 9986-9987 NN denotes %
T17095 9988-10004 NN denotes paraformaldehyde
T17096 10004-10006 , denotes ,
T17097 10006-10013 VBD denotes blocked
T17098 10014-10016 IN denotes in
T17099 10017-10020 CD denotes 0.5
T17100 10020-10021 NN denotes %
T17102 10022-10025 NN denotes BSA
T17103 10025-10026 HYPH denotes /
T17101 10026-10029 NN denotes PBS
T17104 10030-10033 CC denotes and
T17105 10034-10041 VBD denotes stained
T17106 10042-10046 IN denotes with
T17107 10047-10049 DT denotes an
T17109 10050-10057 JJ denotes anti-F4
T17111 10057-10058 HYPH denotes /
T17110 10058-10060 CD denotes 80
T17108 10061-10069 NN denotes antibody
T17112 10070-10071 -LRB- denotes (
T17113 10071-10078 NNP denotes Serotec
T17114 10078-10079 -RRB- denotes )
T17115 10080-10088 VBN denotes followed
T17116 10089-10091 IN denotes by
T17117 10092-10093 DT denotes a
T17119 10094-10103 JJ denotes secondary
T17118 10104-10112 NN denotes antibody
T17120 10113-10120 VBN denotes coupled
T17121 10121-10123 IN denotes to
T17122 10124-10129 NNP denotes Alexa
T17123 10130-10133 CD denotes 488
T17124 10134-10135 -LRB- denotes (
T17126 10135-10144 NNP denotes Molecular
T17127 10145-10151 NNP denotes Probes
T17125 10152-10156 NNP denotes Inc.
T17128 10156-10158 , denotes ,
T17129 10158-10164 NNP denotes Eugene
T17130 10164-10166 , denotes ,
T17131 10166-10169 NNP denotes USA
T17132 10169-10170 -RRB- denotes )
T17133 10170-10171 . denotes .
T17134 10171-10273 sentence denotes Coverslips were mounted on slides and engulfed thymocytes were enumerated by fluorescence microscopy.
T17135 10172-10182 NNS denotes Coverslips
T17137 10183-10187 VBD denotes were
T17136 10188-10195 VBN denotes mounted
T17138 10196-10198 IN denotes on
T17139 10199-10205 NNS denotes slides
T17140 10206-10209 CC denotes and
T17141 10210-10218 VBN denotes engulfed
T17142 10219-10229 NNS denotes thymocytes
T17144 10230-10234 VBD denotes were
T17143 10235-10245 VBN denotes enumerated
T17145 10246-10248 IN denotes by
T17146 10249-10261 NN denotes fluorescence
T17147 10262-10272 NN denotes microscopy
T17148 10272-10273 . denotes .
T17149 10273-10438 sentence denotes The percentage of phagocytosis was calculated by counting at least 300 macrophages and determining the number of macrophages that had engulfed apoptotic thymocytes.
T17150 10274-10277 DT denotes The
T17151 10278-10288 NN denotes percentage
T17153 10289-10291 IN denotes of
T17154 10292-10304 NN denotes phagocytosis
T17155 10305-10308 VBD denotes was
T17152 10309-10319 VBN denotes calculated
T17156 10320-10322 IN denotes by
T17157 10323-10331 VBG denotes counting
T17158 10332-10334 RB denotes at
T17160 10335-10340 RBS denotes least
T17159 10341-10344 CD denotes 300
T17161 10345-10356 NNS denotes macrophages
T17162 10357-10360 CC denotes and
T17163 10361-10372 VBG denotes determining
T17164 10373-10376 DT denotes the
T17165 10377-10383 NN denotes number
T17166 10384-10386 IN denotes of
T17167 10387-10398 NNS denotes macrophages
T17168 10399-10403 WDT denotes that
T17170 10404-10407 VBD denotes had
T17169 10408-10416 VBN denotes engulfed
T17171 10417-10426 JJ denotes apoptotic
T17172 10427-10437 NNS denotes thymocytes
T17173 10437-10438 . denotes .
T17174 10438-10697 sentence denotes The phagocytotic index was calculated according to the following formula: phagocytotic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.
T17175 10439-10442 DT denotes The
T17177 10443-10455 JJ denotes phagocytotic
T17176 10456-10461 NN denotes index
T17179 10462-10465 VBD denotes was
T17178 10466-10476 VBN denotes calculated
T17181 10477-10486 VBG denotes according
T17182 10487-10489 IN denotes to
T17183 10490-10493 DT denotes the
T17185 10494-10503 VBG denotes following
T17184 10504-10511 NN denotes formula
T17186 10511-10513 : denotes :
T17187 10513-10525 JJ denotes phagocytotic
T17188 10526-10531 NN denotes index
T17189 10532-10533 SYM denotes =
T17190 10534-10535 -LRB- denotes (
T17191 10535-10540 JJ denotes total
T17180 10541-10547 NN denotes number
T17192 10548-10550 IN denotes of
T17193 10551-10559 VBN denotes engulfed
T17194 10560-10565 NNS denotes cells
T17195 10565-10566 SYM denotes /
T17197 10566-10571 JJ denotes total
T17196 10572-10578 NN denotes number
T17198 10579-10581 IN denotes of
T17199 10582-10589 VBN denotes counted
T17200 10590-10601 NNS denotes macrophages
T17201 10601-10602 -RRB- denotes )
T17202 10603-10604 SYM denotes ×
T17204 10605-10606 -LRB- denotes (
T17203 10606-10612 NN denotes number
T17205 10613-10615 IN denotes of
T17206 10616-10627 NNS denotes macrophages
T17207 10628-10638 VBG denotes containing
T17208 10639-10647 VBN denotes engulfed
T17209 10648-10653 NNS denotes cells
T17210 10653-10654 SYM denotes /
T17212 10654-10659 JJ denotes total
T17211 10660-10666 NN denotes number
T17213 10667-10669 IN denotes of
T17214 10670-10677 VBN denotes counted
T17215 10678-10689 NNS denotes macrophages
T17216 10689-10690 -RRB- denotes )
T17217 10691-10692 SYM denotes ×
T17218 10693-10696 CD denotes 100
T17219 10696-10697 . denotes .
T17220 10697-10835 sentence denotes The experiments were performed at least three times, each time in triplicate, and the counting was done by three different investigators.
T17221 10698-10701 DT denotes The
T17222 10702-10713 NNS denotes experiments
T17224 10714-10718 VBD denotes were
T17223 10719-10728 VBN denotes performed
T17225 10729-10731 RB denotes at
T17227 10732-10737 RBS denotes least
T17226 10738-10743 CD denotes three
T17228 10744-10749 NNS denotes times
T17229 10749-10751 , denotes ,
T17230 10751-10755 DT denotes each
T17231 10756-10760 NN denotes time
T17232 10761-10763 IN denotes in
T17233 10764-10774 NN denotes triplicate
T17234 10774-10776 , denotes ,
T17235 10776-10779 CC denotes and
T17236 10780-10783 DT denotes the
T17237 10784-10792 NN denotes counting
T17239 10793-10796 VBD denotes was
T17238 10797-10801 VBN denotes done
T17240 10802-10804 IN denotes by
T17241 10805-10810 CD denotes three
T17243 10811-10820 JJ denotes different
T17242 10821-10834 NNS denotes investigators
T17244 10834-10835 . denotes .
T17305 10837-10848 NN denotes Measurement
T17306 10849-10851 IN denotes of
T17307 10852-10862 NN denotes macrophage
T17308 10863-10871 NN denotes cytokine
T17309 10872-10882 NN denotes production
T17310 10882-10969 sentence denotes Monolayer cultures of FLDMs and apoptotic thymocytes were prepared as described above.
T17311 10883-10892 NN denotes Monolayer
T17312 10893-10901 NNS denotes cultures
T17314 10902-10904 IN denotes of
T17315 10905-10910 NNS denotes FLDMs
T17316 10911-10914 CC denotes and
T17317 10915-10924 JJ denotes apoptotic
T17318 10925-10935 NNS denotes thymocytes
T17319 10936-10940 VBD denotes were
T17313 10941-10949 VBN denotes prepared
T17320 10950-10952 IN denotes as
T17321 10953-10962 VBN denotes described
T17322 10963-10968 RB denotes above
T17323 10968-10969 . denotes .
T17324 10969-11138 sentence denotes FLDMs were incubated with medium, LPS (10 ng/ml), apoptotic cells (ratio 1:10) or both for the determination of IL-10, TGF-β1 or TNF-α levels after co-culture for 22 h.
T17325 10970-10975 NNS denotes FLDMs
T17327 10976-10980 VBD denotes were
T17326 10981-10990 VBN denotes incubated
T17328 10991-10995 IN denotes with
T17329 10996-11002 NN denotes medium
T17330 11002-11004 , denotes ,
T17331 11004-11007 NN denotes LPS
T17332 11008-11009 -LRB- denotes (
T17334 11009-11011 CD denotes 10
T17333 11012-11014 NNS denotes ng
T17335 11014-11015 SYM denotes /
T17336 11015-11017 NN denotes ml
T17337 11017-11018 -RRB- denotes )
T17338 11018-11020 , denotes ,
T17339 11020-11029 JJ denotes apoptotic
T17340 11030-11035 NNS denotes cells
T17341 11036-11037 -LRB- denotes (
T17343 11037-11042 NN denotes ratio
T17342 11043-11044 CD denotes 1
T17344 11044-11045 SYM denotes :
T17345 11045-11047 CD denotes 10
T17346 11047-11048 -RRB- denotes )
T17347 11049-11051 CC denotes or
T17348 11052-11056 DT denotes both
T17349 11057-11060 IN denotes for
T17350 11061-11064 DT denotes the
T17351 11065-11078 NN denotes determination
T17352 11079-11081 IN denotes of
T17353 11082-11084 NN denotes IL
T17355 11084-11085 HYPH denotes -
T17356 11085-11087 CD denotes 10
T17357 11087-11089 , denotes ,
T17358 11089-11092 NN denotes TGF
T17360 11092-11093 HYPH denotes -
T17359 11093-11095 NN denotes β1
T17361 11096-11098 CC denotes or
T17362 11099-11102 NN denotes TNF
T17364 11102-11103 HYPH denotes -
T17363 11103-11104 NN denotes α
T17354 11105-11111 NNS denotes levels
T17365 11112-11117 IN denotes after
T17366 11118-11128 NN denotes co-culture
T17367 11129-11132 IN denotes for
T17368 11133-11135 CD denotes 22
T17369 11136-11137 NNS denotes h
T17370 11137-11138 . denotes .
T17371 11138-11253 sentence denotes For TNF-α quantification at various time points, FLDMs were cultured with a high concentration of LPS (100 ng/ml).
T17372 11139-11142 IN denotes For
T17374 11143-11146 NN denotes TNF
T17376 11146-11147 HYPH denotes -
T17375 11147-11148 NN denotes α
T17377 11149-11163 NN denotes quantification
T17378 11164-11166 IN denotes at
T17379 11167-11174 JJ denotes various
T17381 11175-11179 NN denotes time
T17380 11180-11186 NNS denotes points
T17382 11186-11188 , denotes ,
T17383 11188-11193 NNS denotes FLDMs
T17384 11194-11198 VBD denotes were
T17373 11199-11207 VBN denotes cultured
T17385 11208-11212 IN denotes with
T17386 11213-11214 DT denotes a
T17388 11215-11219 JJ denotes high
T17387 11220-11233 NN denotes concentration
T17389 11234-11236 IN denotes of
T17390 11237-11240 NN denotes LPS
T17391 11241-11242 -LRB- denotes (
T17393 11242-11245 CD denotes 100
T17392 11246-11248 NNS denotes ng
T17394 11248-11249 SYM denotes /
T17395 11249-11251 NN denotes ml
T17396 11251-11252 -RRB- denotes )
T17397 11252-11253 . denotes .
T17398 11253-11471 sentence denotes Culture supernatants were harvested and TNF-α (Mouse TNF-α OptEIA set; BD Biosciences, Heidelberg, Germany) and TGF-β1 (Quantikine, TGF-β1 immunoassay; R&D Systems) were measured by ELISA as described by the supplier.
T17399 11254-11261 NN denotes Culture
T17400 11262-11274 NNS denotes supernatants
T17402 11275-11279 VBD denotes were
T17401 11280-11289 VBN denotes harvested
T17403 11290-11293 CC denotes and
T17404 11294-11297 NN denotes TNF
T17406 11297-11298 HYPH denotes -
T17405 11298-11299 NN denotes α
T17408 11300-11301 -LRB- denotes (
T17410 11301-11306 NN denotes Mouse
T17412 11307-11310 NN denotes TNF
T17414 11310-11311 HYPH denotes -
T17413 11311-11312 NN denotes α
T17415 11313-11319 NN denotes OptEIA
T17411 11320-11323 NN denotes set
T17416 11323-11324 : denotes ;
T17417 11325-11327 NNP denotes BD
T17409 11328-11339 NNP denotes Biosciences
T17418 11339-11341 , denotes ,
T17419 11341-11351 NNP denotes Heidelberg
T17420 11351-11353 , denotes ,
T17421 11353-11360 NNP denotes Germany
T17422 11360-11361 -RRB- denotes )
T17423 11362-11365 CC denotes and
T17424 11366-11369 NN denotes TGF
T17426 11369-11370 HYPH denotes -
T17425 11370-11372 NN denotes β1
T17427 11373-11374 -LRB- denotes (
T17429 11374-11384 NNP denotes Quantikine
T17430 11384-11386 , denotes ,
T17431 11386-11389 NN denotes TGF
T17433 11389-11390 HYPH denotes -
T17432 11390-11392 NN denotes β1
T17434 11393-11404 NN denotes immunoassay
T17435 11404-11405 : denotes ;
T17436 11406-11407 NNP denotes R
T17437 11407-11408 CC denotes &
T17438 11408-11409 NNP denotes D
T17428 11410-11417 NNP denotes Systems
T17439 11417-11418 -RRB- denotes )
T17440 11419-11423 VBD denotes were
T17407 11424-11432 VBN denotes measured
T17441 11433-11435 IN denotes by
T17442 11436-11441 NN denotes ELISA
T17443 11442-11444 IN denotes as
T17444 11445-11454 VBN denotes described
T17445 11455-11457 IN denotes by
T17446 11458-11461 DT denotes the
T17447 11462-11470 NN denotes supplier
T17448 11470-11471 . denotes .
T17449 11471-11612 sentence denotes IL-10 in culture supernatants was determined by a cytometric bead assay (Mouse inflammation CBA; BD Biosciences) as indicated in the manual.
T17450 11472-11474 NN denotes IL
T17452 11474-11475 HYPH denotes -
T17453 11475-11477 CD denotes 10
T17454 11478-11480 IN denotes in
T17455 11481-11488 NN denotes culture
T17451 11489-11501 NNS denotes supernatants
T17457 11502-11505 VBD denotes was
T17456 11506-11516 VBN denotes determined
T17458 11517-11519 IN denotes by
T17459 11520-11521 DT denotes a
T17461 11522-11532 JJ denotes cytometric
T17462 11533-11537 NN denotes bead
T17460 11538-11543 NN denotes assay
T17463 11544-11545 -LRB- denotes (
T17465 11545-11550 NN denotes Mouse
T17466 11551-11563 NN denotes inflammation
T17464 11564-11567 NN denotes CBA
T17467 11567-11568 : denotes ;
T17468 11569-11571 NN denotes BD
T17469 11572-11583 NNS denotes Biosciences
T17470 11583-11584 -RRB- denotes )
T17471 11585-11587 IN denotes as
T17472 11588-11597 VBN denotes indicated
T17473 11598-11600 IN denotes in
T17474 11601-11604 DT denotes the
T17475 11605-11611 NN denotes manual
T17476 11611-11612 . denotes .
T17477 11612-11722 sentence denotes Data are presented as mean ± SEM from at least three independent experiments, each carried out in triplicate.
T17478 11613-11617 NNS denotes Data
T17480 11618-11621 VBP denotes are
T17479 11622-11631 VBN denotes presented
T17481 11632-11634 IN denotes as
T17482 11635-11639 NN denotes mean
T17484 11640-11641 SYM denotes ±
T17483 11642-11645 NN denotes SEM
T17485 11646-11650 IN denotes from
T17486 11651-11653 RB denotes at
T17488 11654-11659 RBS denotes least
T17487 11660-11665 CD denotes three
T17490 11666-11677 JJ denotes independent
T17489 11678-11689 NNS denotes experiments
T17491 11689-11691 , denotes ,
T17492 11691-11695 RB denotes each
T17493 11696-11703 VBN denotes carried
T17494 11704-11707 RP denotes out
T17495 11708-11710 IN denotes in
T17496 11711-11721 NN denotes triplicate
T17497 11721-11722 . denotes .
T17498 11722-11831 sentence denotes Analysis of the results used the Wilcoxon-signed rank test; p values below 0.05 were considered significant.
T17499 11723-11731 NN denotes Analysis
T17501 11732-11734 IN denotes of
T17502 11735-11738 DT denotes the
T17503 11739-11746 NNS denotes results
T17500 11747-11751 VBD denotes used
T17505 11752-11755 DT denotes the
T17507 11756-11764 NN denotes Wilcoxon
T17508 11764-11765 HYPH denotes -
T17509 11765-11771 VBN denotes signed
T17510 11772-11776 NN denotes rank
T17506 11777-11781 NN denotes test
T17511 11781-11782 : denotes ;
T17512 11783-11784 NN denotes p
T17513 11785-11791 NNS denotes values
T17514 11792-11797 IN denotes below
T17515 11798-11802 CD denotes 0.05
T17516 11803-11807 VBD denotes were
T17504 11808-11818 VBN denotes considered
T17517 11819-11830 JJ denotes significant
T17518 11830-11831 . denotes .
R10000 T16333 T16287 advcl described,used
R10001 T16334 T16333 agent by,described
R10002 T16335 T16336 det the,supplier
R10003 T16336 T16334 pobj supplier,by
R10004 T16337 T16287 punct .,used
R10005 T16339 T16340 nsubjpass Detection,performed
R10006 T16341 T16340 auxpass was,performed
R10007 T16342 T16340 advcl using,performed
R10008 T16343 T16344 amod indirect,streptavidin
R10009 T16344 T16342 dobj streptavidin,using
R10010 T16345 T16344 prep with,streptavidin
R10011 T16346 T16347 amod biotinylated,antibodies
R10012 T16347 T16345 pobj antibodies,with
R10013 T16348 T16347 amod secondary,antibodies
R10014 T16349 T16344 cc and,streptavidin
R10015 T16350 T16351 npadvmod cobalt,enhanced
R10016 T16351 T16353 amod enhanced,diaminobenzidine
R10017 T16352 T16351 punct -,enhanced
R10018 T16353 T16344 conj diaminobenzidine,streptavidin
R10019 T16354 T16355 punct (,brown
R10020 T16355 T16353 parataxis brown,diaminobenzidine
R10021 T16356 T16355 punct ),brown
R10022 T16357 T16353 cc or,diaminobenzidine
R10023 T16358 T16359 amod fast,red
R10024 T16359 T16353 conj red,diaminobenzidine
R10025 T16360 T16359 punct -,red
R10026 T16361 T16362 punct (,red
R10027 T16362 T16359 parataxis red,red
R10028 T16363 T16362 punct ),red
R10029 T16364 T16353 prep as,diaminobenzidine
R10030 T16365 T16364 pobj chromogens,as
R10031 T16366 T16340 punct .,performed
R10032 T16368 T16369 nsubjpass Sections,counterstained
R10033 T16370 T16369 auxpass were,counterstained
R10034 T16371 T16369 prep with,counterstained
R10035 T16372 T16371 pobj hematoxylin,with
R10036 T16373 T16369 punct .,counterstained
R10037 T16375 T16376 prep For,dissected
R10038 T16377 T16378 amod whole,mount
R10039 T16378 T16380 nmod mount,labeling
R10040 T16379 T16378 punct -,mount
R10041 T16380 T16375 pobj labeling,For
R10042 T16381 T16380 amod terminal,labeling
R10043 T16382 T16380 nmod deoxynucleotidyl,labeling
R10044 T16383 T16384 npadvmod transferase,mediated
R10045 T16384 T16380 amod mediated,labeling
R10046 T16385 T16384 punct -,mediated
R10047 T16386 T16380 compound UTP,labeling
R10048 T16387 T16380 compound end,labeling
R10049 T16388 T16389 punct (,TUNEL
R10050 T16389 T16380 parataxis TUNEL,labeling
R10051 T16390 T16389 punct ),TUNEL
R10052 T16391 T16376 punct ", ",dissected
R10053 T16392 T16393 compound limb,buds
R10054 T16393 T16376 nsubjpass buds,dissected
R10055 T16394 T16376 auxpass were,dissected
R10056 T16395 T16376 prep from,dissected
R10057 T16396 T16397 nmod E12.5,embryos
R10058 T16397 T16395 pobj embryos,from
R10059 T16398 T16396 cc and,E12.5
R10060 T16399 T16396 conj E13.5,E12.5
R10061 T16400 T16376 punct ", ",dissected
R10062 T16401 T16376 conj fixed,dissected
R10063 T16402 T16401 prep in,fixed
R10064 T16403 T16404 nummod 4,%
R10065 T16404 T16405 compound %,paraformaldehyde
R10066 T16405 T16402 pobj paraformaldehyde,in
R10067 T16406 T16401 cc and,fixed
R10068 T16407 T16401 conj processed,fixed
R10069 T16408 T16407 prep for,processed
R10070 T16409 T16408 pobj analysis,for
R10071 T16410 T16411 mark as,described
R10072 T16411 T16407 advcl described,processed
R10073 T16412 T16411 advmod previously,described
R10074 T16413 T16414 punct [,50
R10075 T16414 T16407 parataxis 50,processed
R10076 T16415 T16414 punct ],50
R10077 T16416 T16376 punct .,dissected
R10078 T16465 T16464 prep of,Preparation
R10079 T16466 T16467 amod fetal,liver
R10080 T16467 T16468 npadvmod liver,derived
R10081 T16468 T16470 amod derived,macrophages
R10082 T16469 T16468 punct -,derived
R10083 T16470 T16465 pobj macrophages,of
R10084 T16471 T16470 punct (,macrophages
R10085 T16472 T16470 appos FLDMs,macrophages
R10086 T16473 T16470 punct ),macrophages
R10087 T16475 T16476 amod Fetal,livers
R10088 T16476 T16477 nsubjpass livers,excised
R10089 T16478 T16477 auxpass were,excised
R10090 T16479 T16477 prep from,excised
R10091 T16480 T16479 pobj embryos,from
R10092 T16481 T16477 prep at,excised
R10093 T16482 T16481 pobj E12.5,at
R10094 T16483 T16482 cc and,E12.5
R10095 T16484 T16482 conj E13.5,E12.5
R10096 T16485 T16477 punct ", ",excised
R10097 T16486 T16477 advmod respectively,excised
R10098 T16487 T16477 punct ", ",excised
R10099 T16488 T16477 conj washed,excised
R10100 T16489 T16488 prep in,washed
R10101 T16490 T16489 pobj PBS,in
R10102 T16491 T16488 cc and,washed
R10103 T16492 T16488 conj dissociated,washed
R10104 T16493 T16492 advmod enzymatically,dissociated
R10105 T16494 T16492 prep for,dissociated
R10106 T16495 T16496 nummod 60,min
R10107 T16496 T16494 pobj min,for
R10108 T16497 T16492 prep at,dissociated
R10109 T16498 T16499 nummod 37,°C
R10110 T16499 T16497 pobj °C,at
R10111 T16500 T16477 punct .,excised
R10112 T16502 T16503 det The,buffer
R10113 T16503 T16505 nsubj buffer,comprised
R10114 T16504 T16503 compound digestion,buffer
R10115 T16506 T16507 punct (,μl
R10116 T16507 T16503 parataxis μl,buffer
R10117 T16508 T16507 nummod 150,μl
R10118 T16509 T16507 prep per,μl
R10119 T16510 T16509 pobj liver,per
R10120 T16511 T16507 punct ),μl
R10121 T16512 T16513 nummod 0.6,U
R10122 T16513 T16514 nmod U,dispase
R10123 T16514 T16505 dative dispase,comprised
R10124 T16515 T16516 punct /,ml
R10125 T16516 T16513 prep ml,U
R10126 T16517 T16514 nummod I,dispase
R10127 T16518 T16519 punct (,Roche
R10128 T16519 T16514 parataxis Roche,dispase
R10129 T16520 T16519 punct ),Roche
R10130 T16521 T16505 punct ", ",comprised
R10131 T16522 T16523 nummod 0.1,%
R10132 T16523 T16524 compound %,D
R10133 T16524 T16505 dobj D,comprised
R10134 T16525 T16524 compound collagenase,D
R10135 T16526 T16527 punct (,Roche
R10136 T16527 T16524 parataxis Roche,D
R10137 T16528 T16527 punct ),Roche
R10138 T16529 T16505 punct ", ",comprised
R10139 T16530 T16531 nummod 10,U
R10140 T16531 T16532 compound U,DNase
R10141 T16532 T16505 dobj DNase,comprised
R10142 T16533 T16534 punct (,Roche
R10143 T16534 T16532 parataxis Roche,DNase
R10144 T16535 T16534 punct ),Roche
R10145 T16536 T16505 punct ", ",comprised
R10146 T16537 T16505 cc and,comprised
R10147 T16538 T16539 nummod 20,%
R10148 T16539 T16540 compound %,FCS
R10149 T16540 T16505 conj FCS,comprised
R10150 T16541 T16540 prep in,FCS
R10151 T16542 T16541 pobj PBS,in
R10152 T16543 T16505 punct .,comprised
R10153 T16545 T16546 nmod X,medium
R10154 T16546 T16550 nsubjpass medium,added
R10155 T16547 T16546 punct -,medium
R10156 T16548 T16546 nmod Vivo,medium
R10157 T16549 T16546 nummod 15,medium
R10158 T16551 T16552 punct (,Cambrex
R10159 T16552 T16546 parataxis Cambrex,medium
R10160 T16553 T16552 punct ", ",Cambrex
R10161 T16554 T16555 compound East,Rutherford
R10162 T16555 T16552 npadvmod Rutherford,Cambrex
R10163 T16556 T16552 punct ", ",Cambrex
R10164 T16557 T16552 npadvmod USA,Cambrex
R10165 T16558 T16552 punct ),Cambrex
R10166 T16559 T16550 auxpass was,added
R10167 T16560 T16550 prep to,added
R10168 T16561 T16562 det the,suspension
R10169 T16562 T16560 pobj suspension,to
R10170 T16563 T16562 amod resulting,suspension
R10171 T16564 T16562 compound cell,suspension
R10172 T16565 T16550 punct ", ",added
R10173 T16566 T16550 cc and,added
R10174 T16567 T16568 prep after,resuspended
R10175 T16568 T16550 conj resuspended,added
R10176 T16569 T16567 pobj centrifugation,after
R10177 T16570 T16571 punct (,g
R10178 T16571 T16569 parataxis g,centrifugation
R10179 T16572 T16571 nummod 200,g
R10180 T16573 T16571 punct ×,g
R10181 T16574 T16571 punct ;,g
R10182 T16575 T16576 nummod 3,min
R10183 T16576 T16571 npadvmod min,g
R10184 T16577 T16571 punct ),g
R10185 T16578 T16568 nsubjpass cells,resuspended
R10186 T16579 T16568 auxpass were,resuspended
R10187 T16580 T16568 prep in,resuspended
R10188 T16581 T16582 nmod X,Vivo
R10189 T16582 T16584 nmod Vivo,medium
R10190 T16583 T16582 punct -,Vivo
R10191 T16584 T16580 pobj medium,in
R10192 T16585 T16582 nummod 15,Vivo
R10193 T16586 T16584 acl supplemented,medium
R10194 T16587 T16586 prep with,supplemented
R10195 T16588 T16589 nummod 50,ng
R10196 T16589 T16590 nmod ng,factor
R10197 T16590 T16587 pobj factor,with
R10198 T16591 T16592 punct /,ml
R10199 T16592 T16589 prep ml,ng
R10200 T16593 T16594 compound macrophage,colony
R10201 T16594 T16595 npadvmod colony,stimulating
R10202 T16595 T16590 amod stimulating,factor
R10203 T16596 T16595 punct -,stimulating
R10204 T16597 T16598 punct (,Aldrich
R10205 T16598 T16590 parataxis Aldrich,factor
R10206 T16599 T16600 compound M,CSF
R10207 T16600 T16598 dep CSF,Aldrich
R10208 T16601 T16600 punct -,CSF
R10209 T16602 T16598 punct ;,Aldrich
R10210 T16603 T16598 compound Sigma,Aldrich
R10211 T16604 T16598 punct -,Aldrich
R10212 T16605 T16598 punct ", ",Aldrich
R10213 T16606 T16607 compound St.,Louis
R10214 T16607 T16598 npadvmod Louis,Aldrich
R10215 T16608 T16598 punct ", ",Aldrich
R10216 T16609 T16598 npadvmod USA,Aldrich
R10217 T16610 T16598 punct ),Aldrich
R10218 T16611 T16568 cc and,resuspended
R10219 T16612 T16568 conj cultured,resuspended
R10220 T16613 T16612 prep on,cultured
R10221 T16614 T16615 amod non-treated,culture
R10222 T16615 T16618 compound culture,dishes
R10223 T16616 T16615 compound tissue,culture
R10224 T16617 T16615 punct -,culture
R10225 T16618 T16613 pobj dishes,on
R10226 T16619 T16612 prep at,cultured
R10227 T16620 T16621 nummod 37,°C
R10228 T16621 T16619 pobj °C,at
R10229 T16622 T16612 prep with,cultured
R10230 T16623 T16624 nummod 5,%
R10231 T16624 T16625 compound %,CO2
R10232 T16625 T16622 pobj CO2,with
R10233 T16626 T16568 punct .,resuspended
R10234 T16628 T16629 det Every,day
R10235 T16629 T16633 npadvmod day,changed
R10236 T16630 T16629 amod second,day
R10237 T16631 T16630 cc or,second
R10238 T16632 T16630 conj third,second
R10239 T16634 T16635 det the,medium
R10240 T16635 T16633 nsubjpass medium,changed
R10241 T16636 T16633 auxpass was,changed
R10242 T16637 T16633 prep by,changed
R10243 T16638 T16637 pobj centrifugation,by
R10244 T16639 T16633 punct .,changed
R10245 T16641 T16642 prep Following,cultured
R10246 T16643 T16641 pobj withdrawal,Following
R10247 T16644 T16643 prep of,withdrawal
R10248 T16645 T16646 compound M,CSF
R10249 T16646 T16644 pobj CSF,of
R10250 T16647 T16646 punct -,CSF
R10251 T16648 T16643 prep on,withdrawal
R10252 T16649 T16648 pobj day,on
R10253 T16650 T16649 nummod 6,day
R10254 T16651 T16649 prep after,day
R10255 T16652 T16651 pobj excision,after
R10256 T16653 T16642 punct ", ",cultured
R10257 T16654 T16655 amod adherent,cells
R10258 T16655 T16642 nsubjpass cells,cultured
R10259 T16656 T16642 auxpass were,cultured
R10260 T16657 T16642 prep for,cultured
R10261 T16658 T16659 det an,h
R10262 T16659 T16657 pobj h,for
R10263 T16660 T16659 amod additional,h
R10264 T16661 T16662 compound 24,48
R10265 T16662 T16659 nummod 48,h
R10266 T16663 T16662 punct -,48
R10267 T16664 T16642 prep in,cultured
R10268 T16665 T16666 nmod X,Vivo
R10269 T16666 T16668 nmod Vivo,medium
R10270 T16667 T16666 punct -,Vivo
R10271 T16668 T16664 pobj medium,in
R10272 T16669 T16666 nummod 15,Vivo
R10273 T16670 T16642 punct .,cultured
R10276 T16840 T16841 compound Macrophage,assays
R10277 T16842 T16841 compound phagocytosis,assays
R10278 T16844 T16845 prep For,plated
R10279 T16846 T16844 pobj preparation,For
R10280 T16847 T16846 prep of,preparation
R10281 T16848 T16849 compound monolayer,cultures
R10282 T16849 T16847 pobj cultures,of
R10283 T16850 T16849 prep of,cultures
R10284 T16851 T16850 pobj macrophages,of
R10285 T16852 T16845 punct ", ",plated
R10286 T16853 T16845 nsubjpass FLDMs,plated
R10287 T16854 T16845 auxpass were,plated
R10288 T16855 T16845 prep on,plated
R10289 T16856 T16857 compound glass,coverslips
R10290 T16857 T16855 pobj coverslips,on
R10291 T16858 T16845 prep in,plated
R10292 T16859 T16860 nummod 24,plates
R10293 T16860 T16858 pobj plates,in
R10294 T16861 T16860 compound well,plates
R10295 T16862 T16863 punct (,cells
R10296 T16863 T16858 parataxis cells,in
R10297 T16864 T16865 quantmod 2,105
R10298 T16865 T16863 nummod 105,cells
R10299 T16866 T16865 punct ×,105
R10300 T16867 T16863 prep per,cells
R10301 T16868 T16867 pobj well,per
R10302 T16869 T16863 punct ),cells
R10303 T16870 T16845 prep in,plated
R10304 T16871 T16872 nmod X,Vivo
R10305 T16872 T16874 nmod Vivo,medium
R10306 T16873 T16872 punct -,Vivo
R10307 T16874 T16870 pobj medium,in
R10308 T16875 T16872 nummod 15,Vivo
R10309 T16876 T16845 punct .,plated
R10310 T16878 T16879 prep For,harvested
R10311 T16880 T16878 pobj preparation,For
R10312 T16881 T16880 prep of,preparation
R10313 T16882 T16883 amod apoptotic,cells
R10314 T16883 T16881 pobj cells,of
R10315 T16884 T16883 compound target,cells
R10316 T16885 T16879 punct ", ",harvested
R10317 T16886 T16887 amod primary,thymocytes
R10318 T16887 T16879 nsubjpass thymocytes,harvested
R10319 T16888 T16879 auxpass were,harvested
R10320 T16889 T16879 prep from,harvested
R10321 T16890 T16891 det the,thymus
R10322 T16891 T16889 pobj thymus,from
R10323 T16892 T16891 prep of,thymus
R10324 T16893 T16894 quantmod 4,8
R10325 T16894 T16897 nummod 8,week
R10326 T16895 T16894 punct -,8
R10327 T16896 T16894 quantmod to,8
R10328 T16897 T16899 npadvmod week,old
R10329 T16898 T16897 punct -,week
R10330 T16899 T16901 amod old,mice
R10331 T16900 T16899 punct -,old
R10332 T16901 T16892 pobj mice,of
R10333 T16902 T16903 compound C57BL,6J
R10334 T16903 T16901 compound 6J,mice
R10335 T16904 T16903 punct /,6J
R10336 T16905 T16879 punct ", ",harvested
R10337 T16906 T16879 dep stained,harvested
R10338 T16907 T16906 prep with,stained
R10339 T16908 T16907 pobj TAMRA,with
R10340 T16909 T16906 prep for,stained
R10341 T16910 T16911 nummod 15,min
R10342 T16911 T16909 pobj min,for
R10343 T16912 T16879 punct ", ",harvested
R10344 T16913 T16879 cc and,harvested
R10345 T16914 T16915 nsubjpass apoptosis,induced
R10346 T16915 T16879 conj induced,harvested
R10347 T16916 T16915 auxpass was,induced
R10348 T16917 T16918 preconj either,by
R10349 T16918 T16915 prep by,induced
R10350 T16919 T16918 pcomp treating,by
R10351 T16920 T16919 dobj cells,treating
R10352 T16921 T16919 prep with,treating
R10353 T16922 T16923 nummod 5,μM
R10354 T16923 T16924 compound μM,staurosporine
R10355 T16924 T16921 pobj staurosporine,with
R10356 T16925 T16919 prep in,treating
R10357 T16926 T16925 pobj medium,in
R10358 T16927 T16919 prep for,treating
R10359 T16928 T16929 nummod 4,h
R10360 T16929 T16927 pobj h,for
R10361 T16930 T16919 prep at,treating
R10362 T16931 T16932 nummod 37,°C
R10363 T16932 T16930 pobj °C,at
R10364 T16933 T16918 cc or,by
R10365 T16934 T16918 conj by,by
R10366 T16935 T16934 pcomp culturing,by
R10367 T16936 T16935 dobj cells,culturing
R10368 T16937 T16935 prep in,culturing
R10369 T16938 T16937 pobj medium,in
R10370 T16939 T16935 advmod overnight,culturing
R10371 T16940 T16879 punct .,harvested
R10372 T16942 T16943 det The,efficacy
R10373 T16943 T16944 nsubjpass efficacy,compared
R10374 T16945 T16943 prep of,efficacy
R10375 T16946 T16947 compound apoptosis,induction
R10376 T16947 T16945 pobj induction,of
R10377 T16948 T16944 auxpass was,compared
R10378 T16949 T16944 prep in,compared
R10379 T16950 T16951 amod thymic,cells
R10380 T16951 T16949 pobj cells,in
R10381 T16952 T16951 compound target,cells
R10382 T16953 T16951 cc and,cells
R10383 T16954 T16951 conj controls,cells
R10384 T16955 T16944 prep by,compared
R10385 T16956 T16957 compound FACS,analysis
R10386 T16957 T16955 pobj analysis,by
R10387 T16958 T16944 punct .,compared
R10388 T16960 T16961 prep On,were
R10389 T16962 T16960 amod average,On
R10390 T16963 T16961 punct ", ",were
R10391 T16964 T16965 nummod 60,%
R10392 T16965 T16961 nsubj %,were
R10393 T16966 T16965 prep of,%
R10394 T16967 T16968 det the,cells
R10395 T16968 T16966 pobj cells,of
R10396 T16969 T16965 prep of,%
R10397 T16970 T16971 det the,population
R10398 T16971 T16969 pobj population,of
R10399 T16972 T16971 amod resulting,population
R10400 T16973 T16961 acomp apoptotic,were
R10401 T16974 T16961 punct ", ",were
R10402 T16975 T16961 prep with,were
R10403 T16976 T16977 amod exposed,PS
R10404 T16977 T16975 pobj PS,with
R10405 T16978 T16977 prep on,PS
R10406 T16979 T16980 poss their,surface
R10407 T16980 T16978 pobj surface,on
R10408 T16981 T16961 punct ", ",were
R10409 T16982 T16961 cc and,were
R10410 T16983 T16984 amod less,5
R10411 T16984 T16986 nummod 5,%
R10412 T16985 T16984 quantmod than,5
R10413 T16986 T16987 nsubj %,were
R10414 T16987 T16961 conj were,were
R10415 T16988 T16986 prep of,%
R10416 T16989 T16990 det the,cells
R10417 T16990 T16988 pobj cells,of
R10418 T16991 T16987 acomp necrotic,were
R10419 T16992 T16987 punct ", ",were
R10420 T16993 T16994 mark as,confirmed
R10421 T16994 T16987 advcl confirmed,were
R10422 T16995 T16994 prep by,confirmed
R10423 T16996 T16997 compound FITC,annexin
R10424 T16997 T16995 pobj annexin,by
R10425 T16998 T16997 punct -,annexin
R10426 T16999 T16997 nummod V,annexin
R10427 T17000 T16997 cc and,annexin
R10428 T17001 T17002 compound propidium,iodide
R10429 T17002 T17003 compound iodide,staining
R10430 T17003 T16997 conj staining,annexin
R10431 T17004 T16987 punct .,were
R10432 T17006 T17007 det The,thymocytes
R10433 T17007 T17009 nsubjpass thymocytes,washed
R10434 T17008 T17007 amod apoptotic,thymocytes
R10435 T17010 T17007 acl obtained,thymocytes
R10436 T17011 T17009 auxpass were,washed
R10437 T17012 T17009 prep with,washed
R10438 T17013 T17012 pobj PBS,with
R10439 T17014 T17009 cc and,washed
R10440 T17015 T17009 conj added,washed
R10441 T17016 T17015 prep to,added
R10442 T17017 T17018 det the,cultures
R10443 T17018 T17016 pobj cultures,to
R10444 T17019 T17018 amod prepared,cultures
R10445 T17020 T17018 compound FLDM,cultures
R10446 T17021 T17022 punct (,10
R10447 T17022 T17015 parataxis 10,added
R10448 T17023 T17022 dep ratio,10
R10449 T17024 T17025 punct :,1
R10450 T17025 T17022 prep 1,10
R10451 T17026 T17022 punct ),10
R10452 T17027 T17009 punct .,washed
R10453 T17029 T17030 nsubjpass Phagocytosis,allowed
R10454 T17031 T17030 auxpass was,allowed
R10455 T17032 T17030 advmod then,allowed
R10456 T17033 T17034 aux to,proceed
R10457 T17034 T17030 xcomp proceed,allowed
R10458 T17035 T17034 prep at,proceed
R10459 T17036 T17037 nummod 37,°C
R10460 T17037 T17035 pobj °C,at
R10461 T17038 T17037 cc and,°C
R10462 T17039 T17040 nummod 5,%
R10463 T17040 T17041 compound %,CO2
R10464 T17041 T17037 conj CO2,°C
R10465 T17042 T17030 punct .,allowed
R10466 T17044 T17045 prep After,stopped
R10467 T17046 T17047 det the,periods
R10468 T17047 T17044 pobj periods,After
R10469 T17048 T17047 amod indicated,periods
R10470 T17049 T17047 compound time,periods
R10471 T17050 T17045 punct ", ",stopped
R10472 T17051 T17052 det the,uptake
R10473 T17052 T17045 nsubjpass uptake,stopped
R10474 T17053 T17052 prep of,uptake
R10475 T17054 T17055 amod apoptotic,cells
R10476 T17055 T17053 pobj cells,of
R10477 T17056 T17052 prep by,uptake
R10478 T17057 T17056 pobj FLDMs,by
R10479 T17058 T17045 auxpass was,stopped
R10480 T17059 T17045 prep by,stopped
R10481 T17060 T17061 amod intensive,washing
R10482 T17061 T17059 pobj washing,by
R10483 T17062 T17061 prep of,washing
R10484 T17063 T17062 pobj co-cultures,of
R10485 T17064 T17061 prep with,washing
R10486 T17065 T17066 amod cold,PBS
R10487 T17066 T17064 pobj PBS,with
R10488 T17067 T17068 aux to,remove
R10489 T17068 T17045 advcl remove,stopped
R10490 T17069 T17070 amod unphagocytosed,cells
R10491 T17070 T17068 dobj cells,remove
R10492 T17071 T17045 punct .,stopped
R10493 T17073 T17074 aux To,measure
R10494 T17074 T17075 advcl measure,processed
R10495 T17076 T17074 dobj phagocytosis,measure
R10496 T17077 T17076 prep of,phagocytosis
R10497 T17078 T17079 amod apoptotic,thymocytes
R10498 T17079 T17077 pobj thymocytes,of
R10499 T17080 T17075 punct ", ",processed
R10500 T17081 T17075 nsubjpass macrophages,processed
R10501 T17082 T17075 auxpass were,processed
R10502 T17083 T17075 advmod further,processed
R10503 T17084 T17075 prep for,processed
R10504 T17085 T17086 compound immunofluorescence,analysis
R10505 T17086 T17084 pobj analysis,for
R10506 T17087 T17075 punct .,processed
R10507 T17089 T17090 nsubjpass Cells,fixed
R10508 T17091 T17090 auxpass were,fixed
R10509 T17092 T17090 prep in,fixed
R10510 T17093 T17094 nummod 4,%
R10511 T17094 T17095 compound %,paraformaldehyde
R10512 T17095 T17092 pobj paraformaldehyde,in
R10513 T17096 T17090 punct ", ",fixed
R10514 T17097 T17090 conj blocked,fixed
R10515 T17098 T17097 prep in,blocked
R10516 T17099 T17100 nummod 0.5,%
R10517 T17100 T17101 compound %,PBS
R10518 T17101 T17098 pobj PBS,in
R10519 T17102 T17101 compound BSA,PBS
R10520 T17103 T17101 punct /,PBS
R10521 T17104 T17097 cc and,blocked
R10522 T17105 T17097 conj stained,blocked
R10523 T17106 T17105 prep with,stained
R10524 T17107 T17108 det an,antibody
R10525 T17108 T17106 pobj antibody,with
R10526 T17109 T17110 amod anti-F4,80
R10527 T17110 T17108 nummod 80,antibody
R10528 T17111 T17110 punct /,80
R10529 T17112 T17113 punct (,Serotec
R10530 T17113 T17108 parataxis Serotec,antibody
R10531 T17114 T17113 punct ),Serotec
R10532 T17115 T17108 acl followed,antibody
R10533 T17116 T17115 agent by,followed
R10534 T17117 T17118 det a,antibody
R10535 T17118 T17116 pobj antibody,by
R10536 T17119 T17118 amod secondary,antibody
R10537 T17120 T17118 acl coupled,antibody
R10538 T17121 T17120 prep to,coupled
R10539 T17122 T17121 pobj Alexa,to
R10540 T17123 T17122 nummod 488,Alexa
R10541 T17124 T17125 punct (,Inc.
R10542 T17125 T17122 parataxis Inc.,Alexa
R10543 T17126 T17125 compound Molecular,Inc.
R10544 T17127 T17125 compound Probes,Inc.
R10545 T17128 T17125 punct ", ",Inc.
R10546 T17129 T17125 npadvmod Eugene,Inc.
R10547 T17130 T17125 punct ", ",Inc.
R10548 T17131 T17125 npadvmod USA,Inc.
R10549 T17132 T17125 punct ),Inc.
R10550 T17133 T17090 punct .,fixed
R10551 T17135 T17136 nsubjpass Coverslips,mounted
R10552 T17137 T17136 auxpass were,mounted
R10553 T17138 T17136 prep on,mounted
R10554 T17139 T17138 pobj slides,on
R10555 T17140 T17136 cc and,mounted
R10556 T17141 T17142 amod engulfed,thymocytes
R10557 T17142 T17143 nsubjpass thymocytes,enumerated
R10558 T17143 T17136 conj enumerated,mounted
R10559 T17144 T17143 auxpass were,enumerated
R10560 T17145 T17143 prep by,enumerated
R10561 T17146 T17147 compound fluorescence,microscopy
R10562 T17147 T17145 pobj microscopy,by
R10563 T17148 T17143 punct .,enumerated
R10564 T17150 T17151 det The,percentage
R10565 T17151 T17152 nsubjpass percentage,calculated
R10566 T17153 T17151 prep of,percentage
R10567 T17154 T17153 pobj phagocytosis,of
R10568 T17155 T17152 auxpass was,calculated
R10569 T17156 T17152 prep by,calculated
R10570 T17157 T17156 pcomp counting,by
R10571 T17158 T17159 advmod at,300
R10572 T17159 T17161 nummod 300,macrophages
R10573 T17160 T17159 advmod least,300
R10574 T17161 T17157 dobj macrophages,counting
R10575 T17162 T17157 cc and,counting
R10576 T17163 T17157 conj determining,counting
R10577 T17164 T17165 det the,number
R10578 T17165 T17163 dobj number,determining
R10579 T17166 T17165 prep of,number
R10580 T17167 T17166 pobj macrophages,of
R10581 T17168 T17169 dep that,engulfed
R10582 T17169 T17167 relcl engulfed,macrophages
R10583 T17170 T17169 aux had,engulfed
R10584 T17171 T17172 amod apoptotic,thymocytes
R10585 T17172 T17169 dobj thymocytes,engulfed
R10586 T17173 T17152 punct .,calculated
R10587 T17175 T17176 det The,index
R10588 T17176 T17178 nsubjpass index,calculated
R10589 T17177 T17176 amod phagocytotic,index
R10590 T17178 T17180 ccomp calculated,number
R10591 T17179 T17178 auxpass was,calculated
R10592 T17181 T17178 prep according,calculated
R10593 T17182 T17181 prep to,according
R10594 T17183 T17184 det the,formula
R10595 T17184 T17182 pobj formula,to
R10596 T17185 T17184 amod following,formula
R10597 T17186 T17180 punct : ,number
R10598 T17187 T17188 amod phagocytotic,index
R10599 T17188 T17180 nsubj index,number
R10600 T17189 T17180 punct =,number
R10601 T17190 T17180 punct (,number
R10602 T17191 T17180 amod total,number
R10603 T17192 T17180 prep of,number
R10604 T17193 T17194 amod engulfed,cells
R10605 T17194 T17192 pobj cells,of
R10606 T17195 T17196 punct /,number
R10607 T17196 T17180 prep number,number
R10608 T17197 T17196 amod total,number
R10609 T17198 T17196 prep of,number
R10610 T17199 T17200 amod counted,macrophages
R10611 T17200 T17198 pobj macrophages,of
R10612 T17201 T17180 punct ),number
R10613 T17202 T17203 punct ×,number
R10614 T17203 T17180 prep number,number
R10615 T17204 T17203 punct (,number
R10616 T17205 T17203 prep of,number
R10617 T17206 T17205 pobj macrophages,of
R10618 T17207 T17206 acl containing,macrophages
R10619 T17208 T17209 amod engulfed,cells
R10620 T17209 T17207 dobj cells,containing
R10621 T17210 T17211 punct /,number
R10622 T17211 T17203 prep number,number
R10623 T17212 T17211 amod total,number
R10624 T17213 T17211 prep of,number
R10625 T17214 T17215 amod counted,macrophages
R10626 T17215 T17213 pobj macrophages,of
R10627 T17216 T17203 punct ),number
R10628 T17217 T17218 punct ×,100
R10629 T17218 T17180 prep 100,number
R10630 T17219 T17180 punct .,number
R10631 T17221 T17222 det The,experiments
R10632 T17222 T17223 nsubjpass experiments,performed
R10633 T17224 T17223 auxpass were,performed
R10634 T17225 T17226 advmod at,three
R10635 T17226 T17228 nummod three,times
R10636 T17227 T17226 advmod least,three
R10637 T17228 T17223 npadvmod times,performed
R10638 T17229 T17223 punct ", ",performed
R10639 T17230 T17231 det each,time
R10640 T17231 T17223 npadvmod time,performed
R10641 T17232 T17231 prep in,time
R10642 T17233 T17232 pobj triplicate,in
R10643 T17234 T17223 punct ", ",performed
R10644 T17235 T17223 cc and,performed
R10645 T17236 T17237 det the,counting
R10646 T17237 T17238 nsubjpass counting,done
R10647 T17238 T17223 conj done,performed
R10648 T17239 T17238 auxpass was,done
R10649 T17240 T17238 agent by,done
R10650 T17241 T17242 nummod three,investigators
R10651 T17242 T17240 pobj investigators,by
R10652 T17243 T17242 amod different,investigators
R10653 T17244 T17238 punct .,done
R10654 T17306 T17305 prep of,Measurement
R10655 T17307 T17308 compound macrophage,cytokine
R10656 T17308 T17309 compound cytokine,production
R10657 T17309 T17306 pobj production,of
R10658 T17311 T17312 compound Monolayer,cultures
R10659 T17312 T17313 nsubjpass cultures,prepared
R10660 T17314 T17312 prep of,cultures
R10661 T17315 T17314 pobj FLDMs,of
R10662 T17316 T17315 cc and,FLDMs
R10663 T17317 T17318 amod apoptotic,thymocytes
R10664 T17318 T17315 conj thymocytes,FLDMs
R10665 T17319 T17313 auxpass were,prepared
R10666 T17320 T17321 mark as,described
R10667 T17321 T17313 advcl described,prepared
R10668 T17322 T17321 advmod above,described
R10669 T17323 T17313 punct .,prepared
R10670 T17325 T17326 nsubjpass FLDMs,incubated
R10671 T17327 T17326 auxpass were,incubated
R10672 T17328 T17326 prep with,incubated
R10673 T17329 T17328 pobj medium,with
R10674 T17330 T17329 punct ", ",medium
R10675 T17331 T17329 conj LPS,medium
R10676 T17332 T17333 punct (,ng
R10677 T17333 T17331 parataxis ng,LPS
R10678 T17334 T17333 nummod 10,ng
R10679 T17335 T17336 punct /,ml
R10680 T17336 T17333 prep ml,ng
R10681 T17337 T17333 punct ),ng
R10682 T17338 T17331 punct ", ",LPS
R10683 T17339 T17340 amod apoptotic,cells
R10684 T17340 T17331 conj cells,LPS
R10685 T17341 T17342 punct (,1
R10686 T17342 T17340 parataxis 1,cells
R10687 T17343 T17342 dep ratio,1
R10688 T17344 T17345 punct :,10
R10689 T17345 T17342 prep 10,1
R10690 T17346 T17342 punct ),1
R10691 T17347 T17340 cc or,cells
R10692 T17348 T17340 conj both,cells
R10693 T17349 T17326 prep for,incubated
R10694 T17350 T17351 det the,determination
R10695 T17351 T17349 pobj determination,for
R10696 T17352 T17351 prep of,determination
R10697 T17353 T17354 nmod IL,levels
R10698 T17354 T17352 pobj levels,of
R10699 T17355 T17353 punct -,IL
R10700 T17356 T17353 nummod 10,IL
R10701 T17357 T17353 punct ", ",IL
R10702 T17358 T17359 compound TGF,β1
R10703 T17359 T17353 conj β1,IL
R10704 T17360 T17359 punct -,β1
R10705 T17361 T17359 cc or,β1
R10706 T17362 T17363 compound TNF,α
R10707 T17363 T17359 conj α,β1
R10708 T17364 T17363 punct -,α
R10709 T17365 T17326 prep after,incubated
R10710 T17366 T17365 pobj co-culture,after
R10711 T17367 T17366 prep for,co-culture
R10712 T17368 T17369 nummod 22,h
R10713 T17369 T17367 pobj h,for
R10714 T17370 T17326 punct .,incubated
R10715 T17372 T17373 prep For,cultured
R10716 T17374 T17375 compound TNF,α
R10717 T17375 T17377 compound α,quantification
R10718 T17376 T17375 punct -,α
R10719 T17377 T17372 pobj quantification,For
R10720 T17378 T17377 prep at,quantification
R10721 T17379 T17380 amod various,points
R10722 T17380 T17378 pobj points,at
R10723 T17381 T17380 compound time,points
R10724 T17382 T17373 punct ", ",cultured
R10725 T17383 T17373 nsubjpass FLDMs,cultured
R10726 T17384 T17373 auxpass were,cultured
R10727 T17385 T17373 prep with,cultured
R10728 T17386 T17387 det a,concentration
R10729 T17387 T17385 pobj concentration,with
R10730 T17388 T17387 amod high,concentration
R10731 T17389 T17387 prep of,concentration
R10732 T17390 T17389 pobj LPS,of
R10733 T17391 T17392 punct (,ng
R10734 T17392 T17387 parataxis ng,concentration
R10735 T17393 T17392 nummod 100,ng
R10736 T17394 T17395 punct /,ml
R10737 T17395 T17392 prep ml,ng
R10738 T17396 T17392 punct ),ng
R10739 T17397 T17373 punct .,cultured
R10740 T17399 T17400 compound Culture,supernatants
R10741 T17400 T17401 nsubjpass supernatants,harvested
R10742 T17402 T17401 auxpass were,harvested
R10743 T17403 T17401 cc and,harvested
R10744 T17404 T17405 compound TNF,α
R10745 T17405 T17407 nsubjpass α,measured
R10746 T17406 T17405 punct -,α
R10747 T17407 T17401 conj measured,harvested
R10748 T17408 T17409 punct (,Biosciences
R10749 T17409 T17405 parataxis Biosciences,α
R10750 T17410 T17411 compound Mouse,set
R10751 T17411 T17409 dep set,Biosciences
R10752 T17412 T17413 compound TNF,α
R10753 T17413 T17411 compound α,set
R10754 T17414 T17413 punct -,α
R10755 T17415 T17411 compound OptEIA,set
R10756 T17416 T17409 punct ;,Biosciences
R10757 T17417 T17409 compound BD,Biosciences
R10758 T17418 T17409 punct ", ",Biosciences
R10759 T17419 T17409 npadvmod Heidelberg,Biosciences
R10760 T17420 T17409 punct ", ",Biosciences
R10761 T17421 T17409 npadvmod Germany,Biosciences
R10762 T17422 T17409 punct ),Biosciences
R10763 T17423 T17405 cc and,α
R10764 T17424 T17425 compound TGF,β1
R10765 T17425 T17405 conj β1,α
R10766 T17426 T17425 punct -,β1
R10767 T17427 T17428 punct (,Systems
R10768 T17428 T17425 parataxis Systems,β1
R10769 T17429 T17428 dep Quantikine,Systems
R10770 T17430 T17428 punct ", ",Systems
R10771 T17431 T17432 compound TGF,β1
R10772 T17432 T17434 compound β1,immunoassay
R10773 T17433 T17432 punct -,β1
R10774 T17434 T17428 dep immunoassay,Systems
R10775 T17435 T17428 punct ;,Systems
R10776 T17436 T17428 nmod R,Systems
R10777 T17437 T17436 cc &,R
R10778 T17438 T17436 conj D,R
R10779 T17439 T17428 punct ),Systems
R10780 T17440 T17407 auxpass were,measured
R10781 T17441 T17407 prep by,measured
R10782 T17442 T17441 pobj ELISA,by
R10783 T17443 T17444 mark as,described
R10784 T17444 T17407 advcl described,measured
R10785 T17445 T17444 agent by,described
R10786 T17446 T17447 det the,supplier
R10787 T17447 T17445 pobj supplier,by
R10788 T17448 T17407 punct .,measured
R10789 T17450 T17451 nmod IL,supernatants
R10790 T17451 T17456 nsubjpass supernatants,determined
R10791 T17452 T17450 punct -,IL
R10792 T17453 T17450 nummod 10,IL
R10793 T17454 T17455 nmod in,culture
R10794 T17455 T17451 compound culture,supernatants
R10795 T17457 T17456 auxpass was,determined
R10796 T17458 T17456 prep by,determined
R10797 T17459 T17460 det a,assay
R10798 T17460 T17458 pobj assay,by
R10799 T17461 T17460 amod cytometric,assay
R10800 T17462 T17460 compound bead,assay
R10801 T17463 T17464 punct (,CBA
R10802 T17464 T17460 parataxis CBA,assay
R10803 T17465 T17464 compound Mouse,CBA
R10804 T17466 T17464 compound inflammation,CBA
R10805 T17467 T17464 punct ;,CBA
R10806 T17468 T17469 compound BD,Biosciences
R10807 T17469 T17464 npadvmod Biosciences,CBA
R10808 T17470 T17464 punct ),CBA
R10809 T17471 T17472 mark as,indicated
R10810 T17472 T17456 advcl indicated,determined
R10811 T17473 T17472 prep in,indicated
R10812 T17474 T17475 det the,manual
R10813 T17475 T17473 pobj manual,in
R10814 T17476 T17456 punct .,determined
R10815 T17478 T17479 nsubjpass Data,presented
R10816 T17480 T17479 auxpass are,presented
R10817 T17481 T17479 prep as,presented
R10818 T17482 T17483 nmod mean,SEM
R10819 T17483 T17481 pobj SEM,as
R10820 T17484 T17483 punct ±,SEM
R10821 T17485 T17479 prep from,presented
R10822 T17486 T17487 advmod at,three
R10823 T17487 T17489 nummod three,experiments
R10824 T17488 T17487 advmod least,three
R10825 T17489 T17485 pobj experiments,from
R10826 T17490 T17489 amod independent,experiments
R10827 T17491 T17489 punct ", ",experiments
R10828 T17492 T17493 advmod each,carried
R10829 T17493 T17489 acl carried,experiments
R10830 T17494 T17493 prt out,carried
R10831 T17495 T17493 prep in,carried
R10832 T17496 T17495 pobj triplicate,in
R10833 T17497 T17479 punct .,presented
R10834 T17499 T17500 nsubj Analysis,used
R10835 T17500 T17504 ccomp used,considered
R10836 T17501 T17499 prep of,Analysis
R10837 T17502 T17503 det the,results
R10838 T17503 T17501 pobj results,of
R10839 T17505 T17506 det the,test
R10840 T17506 T17500 dobj test,used
R10841 T17507 T17506 nmod Wilcoxon,test
R10842 T17508 T17507 punct -,Wilcoxon
R10843 T17509 T17507 amod signed,Wilcoxon
R10844 T17510 T17506 compound rank,test
R10845 T17511 T17504 punct ;,considered
R10846 T17512 T17513 compound p,values
R10847 T17513 T17504 nsubjpass values,considered
R10848 T17514 T17513 prep below,values
R10849 T17515 T17514 pobj 0.05,below
R10850 T17516 T17504 auxpass were,considered
R10851 T17517 T17504 oprd significant,considered
R10852 T17518 T17504 punct .,considered
R8617 T14459 T14458 prep of,Construction
R8618 T14460 T14461 det the,vector
R8619 T14461 T14459 pobj vector,of
R8620 T14462 T14461 compound targeting,vector
R8621 T14463 T14458 cc and,Construction
R8622 T14464 T14458 conj generation,Construction
R8623 T14465 T14464 prep of,generation
R8624 T14466 T14467 nmod Ptdsr,knockout
R8625 T14467 T14469 nmod knockout,mice
R8626 T14468 T14467 punct -,knockout
R8627 T14469 T14465 pobj mice,of
R8628 T14470 T14467 cc and,knockout
R8629 T14471 T14472 compound gene,trap
R8630 T14472 T14467 conj trap,knockout
R8631 T14473 T14472 punct -,trap
R8632 T14475 T14476 compound Targeting,vector
R8633 T14478 T14479 det A,clone
R8634 T14479 T14489 nsubjpass clone,isolated
R8635 T14480 T14481 npadvmod Ptdsr,containing
R8636 T14481 T14479 amod containing,clone
R8637 T14482 T14481 punct -,containing
R8638 T14483 T14484 amod bacterial,chromosome
R8639 T14484 T14479 nmod chromosome,clone
R8640 T14485 T14484 amod artificial,chromosome
R8641 T14486 T14484 punct (,chromosome
R8642 T14487 T14484 appos BAC,chromosome
R8643 T14488 T14479 punct ),clone
R8644 T14490 T14491 punct (,RP
R8645 T14491 T14479 parataxis RP,clone
R8646 T14492 T14493 compound GenBank,AC091694
R8647 T14493 T14491 dep AC091694,RP
R8648 T14494 T14493 compound accession,AC091694
R8649 T14495 T14493 compound number,AC091694
R8650 T14496 T14491 punct ;,RP
R8651 T14497 T14491 punct -,RP
R8652 T14498 T14491 nummod 23,RP
R8653 T14499 T14491 punct -,RP
R8654 T14500 T14491 nummod 316F3,RP
R8655 T14501 T14491 punct ),RP
R8656 T14502 T14489 auxpass was,isolated
R8657 T14503 T14489 prep by,isolated
R8658 T14504 T14505 compound sequence,homology
R8659 T14505 T14503 pobj homology,by
R8660 T14506 T14505 prep from,homology
R8661 T14507 T14508 det a,library
R8662 T14508 T14506 pobj library,from
R8663 T14509 T14510 nmod C57BL,6J
R8664 T14510 T14508 nmod 6J,library
R8665 T14511 T14510 punct /,6J
R8666 T14512 T14508 amod genomic,library
R8667 T14513 T14508 compound BAC,library
R8668 T14514 T14515 punct (,Resources
R8669 T14515 T14508 parataxis Resources,library
R8670 T14516 T14515 dep RP,Resources
R8671 T14517 T14516 punct -,RP
R8672 T14518 T14516 nummod 23,RP
R8673 T14519 T14515 punct ;,Resources
R8674 T14520 T14515 compound BACPAC,Resources
R8675 T14521 T14515 punct ", ",Resources
R8676 T14522 T14515 npadvmod Oakland,Resources
R8677 T14523 T14515 punct ", ",Resources
R8678 T14524 T14515 npadvmod USA,Resources
R8679 T14525 T14515 punct ),Resources
R8680 T14526 T14489 punct .,isolated
R8681 T14528 T14529 det A,fragment
R8682 T14529 T14535 nsubjpass fragment,subcloned
R8683 T14530 T14531 nummod 14.5,kb
R8684 T14531 T14529 compound kb,fragment
R8685 T14532 T14533 compound KpnI,BamHI
R8686 T14533 T14529 compound BamHI,fragment
R8687 T14534 T14533 punct /,BamHI
R8688 T14536 T14529 acl containing,fragment
R8689 T14537 T14538 det the,locus
R8690 T14538 T14536 dobj locus,containing
R8691 T14539 T14538 amod entire,locus
R8692 T14540 T14538 compound Ptdsr,locus
R8693 T14541 T14538 cc and,locus
R8694 T14542 T14543 nummod 5,regions
R8695 T14543 T14538 conj regions,locus
R8696 T14544 T14542 punct ',5
R8697 T14545 T14542 cc and,5
R8698 T14546 T14542 conj 3,5
R8699 T14547 T14546 punct ',3
R8700 T14548 T14543 compound flanking,regions
R8701 T14549 T14535 auxpass was,subcloned
R8702 T14550 T14535 prep from,subcloned
R8703 T14551 T14552 det this,clone
R8704 T14552 T14550 pobj clone,from
R8705 T14553 T14552 compound BAC,clone
R8706 T14554 T14535 cc and,subcloned
R8707 T14555 T14556 det a,fragment
R8708 T14556 T14562 nsubjpass fragment,replaced
R8709 T14557 T14558 nummod 1.9,kb
R8710 T14558 T14556 compound kb,fragment
R8711 T14559 T14560 compound RsrII,AatII
R8712 T14560 T14556 compound AatII,fragment
R8713 T14561 T14560 punct /,AatII
R8714 T14562 T14535 conj replaced,subcloned
R8715 T14563 T14556 acl containing,fragment
R8716 T14564 T14565 nmod exons,I
R8717 T14565 T14563 dobj I,containing
R8718 T14566 T14565 cc and,I
R8719 T14567 T14565 conj II,I
R8720 T14568 T14565 prep of,I
R8721 T14569 T14570 det the,gene
R8722 T14570 T14568 pobj gene,of
R8723 T14571 T14570 compound Ptdsr,gene
R8724 T14572 T14562 auxpass was,replaced
R8725 T14573 T14562 prep by,replaced
R8726 T14574 T14575 det a,cassette
R8727 T14575 T14573 pobj cassette,by
R8728 T14576 T14577 nummod 1.2,kb
R8729 T14577 T14575 nmod kb,cassette
R8730 T14578 T14579 npadvmod loxP,flanked
R8731 T14579 T14575 amod flanked,cassette
R8732 T14580 T14579 punct -,flanked
R8733 T14581 T14582 compound neomycin,resistance
R8734 T14582 T14575 compound resistance,cassette
R8735 T14583 T14582 punct -,resistance
R8736 T14584 T14575 compound gene,cassette
R8737 T14585 T14586 punct (,neo
R8738 T14586 T14575 parataxis neo,cassette
R8739 T14587 T14586 punct ),neo
R8740 T14588 T14562 punct .,replaced
R8741 T14590 T14591 amod Homologous,recombination
R8742 T14592 T14591 prep in,recombination
R8743 T14593 T14594 compound ES,cells
R8744 T14594 T14592 pobj cells,in
R8745 T14595 T14591 cc and,recombination
R8746 T14596 T14591 conj generation,recombination
R8747 T14597 T14596 prep of,generation
R8748 T14598 T14599 compound germ,line
R8749 T14599 T14601 compound line,chimeras
R8750 T14600 T14599 punct -,line
R8751 T14601 T14597 pobj chimeras,of
R8752 T14603 T14604 compound Bruce4,cells
R8753 T14604 T14606 nsubjpass cells,transfected
R8754 T14605 T14604 compound ES,cells
R8755 T14607 T14606 auxpass were,transfected
R8756 T14608 T14606 prep with,transfected
R8757 T14609 T14610 npadvmod KpnI,linearized
R8758 T14610 T14612 amod linearized,vector
R8759 T14611 T14610 punct -,linearized
R8760 T14612 T14608 pobj vector,with
R8761 T14613 T14612 compound targeting,vector
R8762 T14614 T14606 cc and,transfected
R8763 T14615 T14606 conj selected,transfected
R8764 T14616 T14615 prep with,selected
R8765 T14617 T14616 pobj G418,with
R8766 T14618 T14606 punct .,transfected
R8767 T14620 T14621 compound ES,cell
R8768 T14621 T14623 compound cell,clones
R8769 T14622 T14621 punct -,cell
R8770 T14623 T14624 nsubjpass clones,isolated
R8771 T14625 T14623 amod resistant,clones
R8772 T14626 T14625 prep to,resistant
R8773 T14627 T14626 pobj G418,to
R8774 T14628 T14624 auxpass were,isolated
R8775 T14629 T14624 cc and,isolated
R8776 T14630 T14624 conj analyzed,isolated
R8777 T14631 T14630 prep by,analyzed
R8778 T14632 T14633 compound Southern,blot
R8779 T14633 T14634 compound blot,analysis
R8780 T14634 T14631 pobj analysis,by
R8781 T14635 T14630 prep for,analyzed
R8782 T14636 T14637 amod homologous,events
R8783 T14637 T14635 pobj events,for
R8784 T14638 T14637 compound recombination,events
R8785 T14639 T14637 prep within,events
R8786 T14640 T14641 det the,locus
R8787 T14641 T14639 pobj locus,within
R8788 T14642 T14641 compound Ptdsr,locus
R8789 T14643 T14624 punct .,isolated
R8790 T14645 T14646 amod Chimeric,mice
R8791 T14646 T14647 nsubjpass mice,produced
R8792 T14648 T14647 auxpass were,produced
R8793 T14649 T14647 prep by,produced
R8794 T14650 T14649 pobj microinjection,by
R8795 T14651 T14650 prep of,microinjection
R8796 T14652 T14653 nummod two,cells
R8797 T14653 T14651 pobj cells,of
R8798 T14654 T14653 amod independent,cells
R8799 T14655 T14653 amod homologous,cells
R8800 T14656 T14653 amod recombinant,cells
R8801 T14657 T14658 punct (,Ptdsr
R8802 T14658 T14653 parataxis Ptdsr,cells
R8803 T14659 T14658 punct +,Ptdsr
R8804 T14660 T14658 punct /,Ptdsr
R8805 T14661 T14658 punct -,Ptdsr
R8806 T14662 T14658 punct ),Ptdsr
R8807 T14663 T14653 compound ES,cells
R8808 T14664 T14650 prep into,microinjection
R8809 T14665 T14666 compound BALB,c
R8810 T14666 T14668 compound c,blastocysts
R8811 T14667 T14666 punct /,c
R8812 T14668 T14664 pobj blastocysts,into
R8813 T14669 T14650 cc and,microinjection
R8814 T14670 T14650 conj transfer,microinjection
R8815 T14671 T14670 prep to,transfer
R8816 T14672 T14673 amod pseudopregnant,mothers
R8817 T14673 T14671 pobj mothers,to
R8818 T14674 T14673 amod foster,mothers
R8819 T14675 T14650 acl followed,microinjection
R8820 T14676 T14675 agent by,followed
R8821 T14677 T14676 pobj development,by
R8822 T14678 T14677 prep to,development
R8823 T14679 T14678 pobj term,to
R8824 T14680 T14647 punct .,produced
R8825 T14682 T14683 amod Chimeric,males
R8826 T14683 T14684 nsubjpass males,mated
R8827 T14685 T14684 auxpass were,mated
R8828 T14686 T14684 prep with,mated
R8829 T14687 T14688 compound C57BL,6J
R8830 T14688 T14690 compound 6J,females
R8831 T14689 T14688 punct /,6J
R8832 T14690 T14686 pobj females,with
R8833 T14691 T14684 punct .,mated
R8834 T14693 T14694 prep From,contributed
R8835 T14695 T14696 det the,clones
R8836 T14696 T14693 pobj clones,From
R8837 T14697 T14696 nummod two,clones
R8838 T14698 T14696 amod selected,clones
R8839 T14699 T14700 compound ES,cell
R8840 T14700 T14696 compound cell,clones
R8841 T14701 T14700 punct -,cell
R8842 T14702 T14694 punct ", ",contributed
R8843 T14703 T14694 nsubj one,contributed
R8844 T14704 T14694 advmod successfully,contributed
R8845 T14705 T14694 prep to,contributed
R8846 T14706 T14707 det the,line
R8847 T14707 T14705 pobj line,to
R8848 T14708 T14707 compound germ,line
R8849 T14709 T14707 punct -,line
R8850 T14710 T14694 punct .,contributed
R8851 T14712 T14713 compound Germ,line
R8852 T14713 T14715 compound line,transmission
R8853 T14714 T14713 punct -,line
R8854 T14715 T14716 nsubjpass transmission,verified
R8855 T14717 T14715 prep of,transmission
R8856 T14718 T14719 det the,allele
R8857 T14719 T14717 pobj allele,of
R8858 T14720 T14719 amod mutant,allele
R8859 T14721 T14716 auxpass was,verified
R8860 T14722 T14716 prep by,verified
R8861 T14723 T14722 pobj PCR,by
R8862 T14724 T14723 cc and,PCR
R8863 T14725 T14726 compound Southern,blot
R8864 T14726 T14723 conj blot,PCR
R8865 T14727 T14723 prep of,PCR
R8866 T14728 T14729 amod genomic,DNA
R8867 T14729 T14727 pobj DNA,of
R8868 T14730 T14729 prep from,DNA
R8869 T14731 T14732 amod black,color
R8870 T14732 T14735 compound color,offspring
R8871 T14733 T14732 compound coat,color
R8872 T14734 T14732 punct -,color
R8873 T14735 T14730 pobj offspring,from
R8874 T14736 T14735 compound F1,offspring
R8875 T14737 T14716 punct .,verified
R8876 T14739 T14740 compound Ptdsr,trap
R8877 T14741 T14740 compound gene,trap
R8878 T14742 T14740 punct -,trap
R8879 T14743 T14740 cc and,trap
R8880 T14744 T14740 conj generation,trap
R8881 T14745 T14744 prep of,generation
R8882 T14746 T14747 compound germ,line
R8883 T14747 T14749 compound line,chimeras
R8884 T14748 T14747 punct -,line
R8885 T14749 T14745 pobj chimeras,of
R8886 T14751 T14752 det An,line
R8887 T14752 T14756 nsubjpass line,identified
R8888 T14753 T14754 compound ES,cell
R8889 T14754 T14752 compound cell,line
R8890 T14755 T14754 punct -,cell
R8891 T14757 T14752 acl carrying,line
R8892 T14758 T14759 det a,vector
R8893 T14759 T14757 dobj vector,carrying
R8894 T14760 T14761 compound β,geo
R8895 T14761 T14759 compound geo,vector
R8896 T14762 T14761 punct -,geo
R8897 T14763 T14764 compound gene,trap
R8898 T14764 T14759 compound trap,vector
R8899 T14765 T14764 punct -,trap
R8900 T14766 T14757 prep in,carrying
R8901 T14767 T14768 det the,locus
R8902 T14768 T14766 pobj locus,in
R8903 T14769 T14768 compound Ptdsr,locus
R8904 T14770 T14756 auxpass was,identified
R8905 T14771 T14756 prep by,identified
R8906 T14772 T14771 pcomp searching,by
R8907 T14773 T14774 det the,database
R8908 T14774 T14772 dobj database,searching
R8909 T14775 T14774 compound BayGenomics,database
R8910 T14776 T14777 punct (,48
R8911 T14777 T14774 parataxis 48,database
R8912 T14778 T14777 dep BayGenomics,48
R8913 T14779 T14778 punct ", ",BayGenomics
R8914 T14780 T14781 compound San,Francisco
R8915 T14781 T14778 npadvmod Francisco,BayGenomics
R8916 T14782 T14778 punct ", ",BayGenomics
R8917 T14783 T14778 npadvmod USA,BayGenomics
R8918 T14784 T14777 punct ;,48
R8919 T14785 T14777 punct [,48
R8920 T14786 T14777 punct ],48
R8921 T14787 T14777 punct ),48
R8922 T14788 T14772 prep with,searching
R8923 T14789 T14790 det the,cDNA
R8924 T14790 T14788 pobj cDNA,with
R8925 T14791 T14792 amod full,length
R8926 T14792 T14790 compound length,cDNA
R8927 T14793 T14792 punct -,length
R8928 T14794 T14790 compound Ptdsr,cDNA
R8929 T14795 T14756 punct .,identified
R8930 T14797 T14798 det A,line
R8931 T14798 T14803 nsubjpass line,carrying
R8932 T14799 T14798 amod single,line
R8933 T14800 T14801 compound ES,cell
R8934 T14801 T14798 compound cell,line
R8935 T14802 T14801 punct -,cell
R8936 T14804 T14803 auxpass was,carrying
R8937 T14805 T14803 aux identified,carrying
R8938 T14806 T14807 det the,trap
R8939 T14807 T14803 dobj trap,carrying
R8940 T14808 T14807 compound gene,trap
R8941 T14809 T14807 punct -,trap
R8942 T14810 T14803 prep in,carrying
R8943 T14811 T14810 pobj intron,in
R8944 T14812 T14811 nummod V,intron
R8945 T14813 T14811 punct ", ",intron
R8946 T14814 T14811 prep between,intron
R8947 T14815 T14816 nmod exons,V
R8948 T14816 T14814 pobj V,between
R8949 T14817 T14816 cc and,V
R8950 T14818 T14816 conj VI,V
R8951 T14819 T14816 prep of,V
R8952 T14820 T14821 det the,gene
R8953 T14821 T14819 pobj gene,of
R8954 T14822 T14821 compound Ptdsr,gene
R8955 T14823 T14803 punct .,carrying
R8956 T14825 T14826 amod Chimeric,mice
R8957 T14826 T14827 nsubjpass mice,generated
R8958 T14828 T14827 auxpass were,generated
R8959 T14829 T14827 prep by,generated
R8960 T14830 T14829 pobj microinjection,by
R8961 T14831 T14830 prep into,microinjection
R8962 T14832 T14833 compound CB20,blastocysts
R8963 T14833 T14831 pobj blastocysts,into
R8964 T14834 T14830 cc and,microinjection
R8965 T14835 T14830 conj transfer,microinjection
R8966 T14836 T14835 prep to,transfer
R8967 T14837 T14838 amod pseudopregnant,mothers
R8968 T14838 T14836 pobj mothers,to
R8969 T14839 T14838 amod foster,mothers
R8970 T14840 T14827 punct .,generated
R8971 T14842 T14843 amod Chimeric,males
R8972 T14843 T14844 nsubjpass males,mated
R8973 T14845 T14844 auxpass were,mated
R8974 T14846 T14844 prep with,mated
R8975 T14847 T14848 compound 129P2,OlaHsd
R8976 T14848 T14850 compound OlaHsd,females
R8977 T14849 T14848 punct /,OlaHsd
R8978 T14850 T14846 pobj females,with
R8979 T14851 T14844 punct .,mated
R8980 T14853 T14854 compound Germ,line
R8981 T14854 T14856 compound line,transmission
R8982 T14855 T14854 punct -,line
R8983 T14856 T14857 nsubjpass transmission,verified
R8984 T14858 T14856 prep of,transmission
R8985 T14859 T14860 det the,allele
R8986 T14860 T14858 pobj allele,of
R8987 T14861 T14860 amod mutant,allele
R8988 T14862 T14863 compound gene,trap
R8989 T14863 T14860 compound trap,allele
R8990 T14864 T14863 punct -,trap
R8991 T14865 T14857 auxpass was,verified
R8992 T14866 T14857 prep by,verified
R8993 T14867 T14868 compound expression,analysis
R8994 T14868 T14866 pobj analysis,by
R8995 T14869 T14857 advcl using,verified
R8996 T14870 T14871 compound β,galactosidase
R8997 T14871 T14873 compound galactosidase,staining
R8998 T14872 T14871 punct -,galactosidase
R8999 T14873 T14869 dobj staining,using
R9000 T14874 T14873 cc and,staining
R9001 T14875 T14876 compound RT,PCR
R9002 T14876 T14873 conj PCR,staining
R9003 T14877 T14876 punct -,PCR
R9004 T14878 T14857 punct .,verified
R9009 T15029 T15030 compound Genotype,analysis
R9010 T15032 T15033 det The,genotypes
R9011 T15033 T15034 nsubjpass genotypes,determined
R9012 T15035 T15033 prep of,genotypes
R9013 T15036 T15035 pobj embryos,of
R9014 T15037 T15036 cc or,embryos
R9015 T15038 T15036 conj animals,embryos
R9016 T15039 T15034 auxpass were,determined
R9017 T15040 T15034 prep by,determined
R9018 T15041 T15042 compound PCR,analysis
R9019 T15042 T15040 pobj analysis,by
R9020 T15043 T15034 cc and,determined
R9021 T15044 T15034 conj confirmed,determined
R9022 T15045 T15044 prep by,confirmed
R9023 T15046 T15047 compound Southern,blot
R9024 T15047 T15045 pobj blot,by
R9025 T15048 T15034 punct .,determined
R9026 T15050 T15051 amod Genomic,DNA
R9027 T15051 T15052 nsubjpass DNA,prepared
R9028 T15053 T15051 prep for,DNA
R9029 T15054 T15053 pobj PCR,for
R9030 T15055 T15052 auxpass was,prepared
R9031 T15056 T15052 prep from,prepared
R9032 T15057 T15058 amod extraembryonic,membranes
R9033 T15058 T15056 pobj membranes,from
R9034 T15059 T15058 cc or,membranes
R9035 T15060 T15061 compound tail,clips
R9036 T15061 T15058 conj clips,membranes
R9037 T15062 T15052 advcl using,prepared
R9038 T15063 T15064 det a,protocol
R9039 T15064 T15062 dobj protocol,using
R9040 T15065 T15064 amod non-organic,protocol
R9041 T15066 T15067 compound tail,DNA
R9042 T15067 T15064 compound DNA,protocol
R9043 T15068 T15067 punct -,DNA
R9044 T15069 T15064 compound extraction,protocol
R9045 T15070 T15071 punct [,49
R9046 T15071 T15052 parataxis 49,prepared
R9047 T15072 T15071 punct ],49
R9048 T15073 T15052 punct .,prepared
R9049 T15075 T15076 amod High,weight
R9050 T15076 T15078 nmod weight,DNA
R9051 T15077 T15076 amod molecular,weight
R9052 T15078 T15080 nsubjpass DNA,prepared
R9053 T15079 T15078 amod genomic,DNA
R9054 T15081 T15078 prep for,DNA
R9055 T15082 T15083 compound Southern,blotting
R9056 T15083 T15081 pobj blotting,for
R9057 T15084 T15080 auxpass was,prepared
R9058 T15085 T15080 prep according,prepared
R9059 T15086 T15085 prep to,according
R9060 T15087 T15088 amod standard,protocols
R9061 T15088 T15086 pobj protocols,to
R9062 T15089 T15080 punct .,prepared
R9063 T15091 T15092 prep For,detected
R9064 T15093 T15094 compound PCR,analysis
R9065 T15094 T15091 pobj analysis,For
R9066 T15095 T15096 det the,allele
R9067 T15096 T15092 nsubjpass allele,detected
R9068 T15097 T15098 amod wild,type
R9069 T15098 T15096 compound type,allele
R9070 T15099 T15098 punct -,type
R9071 T15100 T15096 compound Ptdsr,allele
R9072 T15101 T15092 auxpass was,detected
R9073 T15102 T15092 advcl using,detected
R9074 T15103 T15104 advmod forward,primer
R9075 T15104 T15102 dobj primer,using
R9076 T15105 T15104 nummod 1,primer
R9077 T15106 T15107 punct (,GACACTGTCCATGGCAAACAC
R9078 T15107 T15104 parataxis GACACTGTCCATGGCAAACAC,primer
R9079 T15108 T15107 nummod 5,GACACTGTCCATGGCAAACAC
R9080 T15109 T15108 punct ',5
R9081 T15110 T15107 punct -,GACACTGTCCATGGCAAACAC
R9082 T15111 T15107 punct -,GACACTGTCCATGGCAAACAC
R9083 T15112 T15107 nummod 3,GACACTGTCCATGGCAAACAC
R9084 T15113 T15107 punct ',GACACTGTCCATGGCAAACAC
R9085 T15114 T15107 punct ),GACACTGTCCATGGCAAACAC
R9086 T15115 T15104 cc and,primer
R9087 T15116 T15117 amod reverse,primer
R9088 T15117 T15104 conj primer,primer
R9089 T15118 T15117 nummod 2,primer
R9090 T15119 T15120 punct (,TAAAGTCGCCTTCCAGAAGATT
R9091 T15120 T15117 parataxis TAAAGTCGCCTTCCAGAAGATT,primer
R9092 T15121 T15120 nummod 5,TAAAGTCGCCTTCCAGAAGATT
R9093 T15122 T15121 punct ',5
R9094 T15123 T15120 punct -,TAAAGTCGCCTTCCAGAAGATT
R9095 T15124 T15120 punct -,TAAAGTCGCCTTCCAGAAGATT
R9096 T15125 T15120 nummod 3,TAAAGTCGCCTTCCAGAAGATT
R9097 T15126 T15120 punct ',TAAAGTCGCCTTCCAGAAGATT
R9098 T15127 T15120 punct ),TAAAGTCGCCTTCCAGAAGATT
R9099 T15128 T15092 punct .,detected
R9100 T15130 T15131 det The,site
R9101 T15131 T15134 nsubjpass site,located
R9102 T15132 T15131 nmod primer,site
R9103 T15133 T15132 nummod 1,primer
R9104 T15135 T15134 auxpass is,located
R9105 T15136 T15134 npadvmod 5,located
R9106 T15137 T15136 punct ',5
R9107 T15138 T15136 prep to,5
R9108 T15139 T15140 det the,deletion
R9109 T15140 T15138 pobj deletion,to
R9110 T15141 T15134 cc and,located
R9111 T15142 T15143 det the,site
R9112 T15143 T15134 conj site,located
R9113 T15144 T15143 nmod primer,site
R9114 T15145 T15144 nummod 2,primer
R9115 T15146 T15143 prep within,site
R9116 T15147 T15148 det the,deletion
R9117 T15148 T15146 pobj deletion,within
R9118 T15149 T15143 punct .,site
R9119 T15151 T15152 det This,pair
R9120 T15152 T15154 nsubj pair,amplified
R9121 T15153 T15152 compound primer,pair
R9122 T15155 T15156 det a,fragment
R9123 T15156 T15154 dobj fragment,amplified
R9124 T15157 T15156 prep of,fragment
R9125 T15158 T15159 advmod approximately,300
R9126 T15159 T15160 nummod 300,bp
R9127 T15160 T15157 pobj bp,of
R9128 T15161 T15156 prep from,fragment
R9129 T15162 T15163 amod wild,type
R9130 T15163 T15165 nmod type,mice
R9131 T15164 T15163 punct -,type
R9132 T15165 T15161 pobj mice,from
R9133 T15166 T15163 cc and,type
R9134 T15167 T15163 conj Ptdsr,type
R9135 T15168 T15167 punct +,Ptdsr
R9136 T15169 T15167 punct /,Ptdsr
R9137 T15170 T15167 punct -,Ptdsr
R9138 T15171 T15161 cc but,from
R9139 T15172 T15171 neg not,but
R9140 T15173 T15161 conj from,from
R9141 T15174 T15175 nmod Ptdsr,mutants
R9142 T15175 T15173 pobj mutants,from
R9143 T15176 T15174 punct -,Ptdsr
R9144 T15177 T15174 punct /,Ptdsr
R9145 T15178 T15174 punct -,Ptdsr
R9146 T15179 T15154 punct .,amplified
R9147 T15181 T15182 aux To,detect
R9148 T15182 T15183 advcl detect,amplified
R9149 T15184 T15185 det the,allele
R9150 T15185 T15182 dobj allele,detect
R9151 T15186 T15185 amod mutant,allele
R9152 T15187 T15185 compound Ptdsr,allele
R9153 T15188 T15183 punct ", ",amplified
R9154 T15189 T15190 amod genomic,DNA
R9155 T15190 T15183 nsubjpass DNA,amplified
R9156 T15191 T15183 auxpass was,amplified
R9157 T15192 T15183 advmod also,amplified
R9158 T15193 T15183 advcl using,amplified
R9159 T15194 T15193 dobj primer,using
R9160 T15195 T15194 nummod 1,primer
R9161 T15196 T15194 cc and,primer
R9162 T15197 T15198 amod reverse,primer
R9163 T15198 T15194 conj primer,primer
R9164 T15199 T15198 nummod 3,primer
R9165 T15200 T15201 punct (,CCACACGCGTCACCTTAATA
R9166 T15201 T15198 parataxis CCACACGCGTCACCTTAATA,primer
R9167 T15202 T15201 nummod 5,CCACACGCGTCACCTTAATA
R9168 T15203 T15202 punct ',5
R9169 T15204 T15201 punct -,CCACACGCGTCACCTTAATA
R9170 T15205 T15201 punct -,CCACACGCGTCACCTTAATA
R9171 T15206 T15201 nummod 3,CCACACGCGTCACCTTAATA
R9172 T15207 T15201 punct ',CCACACGCGTCACCTTAATA
R9173 T15208 T15201 punct ),CCACACGCGTCACCTTAATA
R9174 T15209 T15198 punct ", ",primer
R9175 T15210 T15211 dep which,corresponds
R9176 T15211 T15198 relcl corresponds,primer
R9177 T15212 T15211 prep to,corresponds
R9178 T15213 T15214 det a,sequence
R9179 T15214 T15212 pobj sequence,to
R9180 T15215 T15214 prep in,sequence
R9181 T15216 T15217 det the,cassette
R9182 T15217 T15215 pobj cassette,in
R9183 T15218 T15217 compound neo,cassette
R9184 T15219 T15183 punct .,amplified
R9185 T15221 T15222 prep In,detected
R9186 T15223 T15224 det this,case
R9187 T15224 T15221 pobj case,In
R9188 T15225 T15222 punct ", ",detected
R9189 T15226 T15227 det a,fragment
R9190 T15227 T15222 nsubjpass fragment,detected
R9191 T15228 T15229 nummod 500,bp
R9192 T15229 T15227 compound bp,fragment
R9193 T15230 T15222 auxpass was,detected
R9194 T15231 T15222 prep in,detected
R9195 T15232 T15231 pobj mice,in
R9196 T15233 T15232 amod heterozygous,mice
R9197 T15234 T15233 cc or,heterozygous
R9198 T15235 T15233 conj homozygous,heterozygous
R9199 T15236 T15235 prep for,homozygous
R9200 T15237 T15238 det the,allele
R9201 T15238 T15236 pobj allele,for
R9202 T15239 T15238 amod mutant,allele
R9203 T15240 T15222 punct ", ",detected
R9204 T15241 T15242 mark while,detected
R9205 T15242 T15222 advcl detected,detected
R9206 T15243 T15244 det no,signal
R9207 T15244 T15242 nsubjpass signal,detected
R9208 T15245 T15242 auxpass was,detected
R9209 T15246 T15242 prep in,detected
R9210 T15247 T15248 amod wild,type
R9211 T15248 T15250 compound type,mice
R9212 T15249 T15248 punct -,type
R9213 T15250 T15246 pobj mice,in
R9214 T15251 T15222 punct .,detected
R9215 T15253 T15254 prep For,digested
R9216 T15255 T15256 compound Southern,blot
R9217 T15256 T15257 compound blot,analysis
R9218 T15257 T15253 pobj analysis,For
R9219 T15258 T15254 punct ", ",digested
R9220 T15259 T15260 amod genomic,DNA
R9221 T15260 T15254 nsubjpass DNA,digested
R9222 T15261 T15262 punct (,μg
R9223 T15262 T15260 parataxis μg,DNA
R9224 T15263 T15262 nummod 30,μg
R9225 T15264 T15262 punct ),μg
R9226 T15265 T15254 auxpass was,digested
R9227 T15266 T15254 advmod overnight,digested
R9228 T15267 T15254 prep with,digested
R9229 T15268 T15267 pobj BamHI,with
R9230 T15269 T15270 punct (,GmbH
R9231 T15270 T15268 parataxis GmbH,BamHI
R9232 T15271 T15272 nummod 30,U
R9233 T15272 T15270 dep U,GmbH
R9234 T15273 T15270 punct ;,GmbH
R9235 T15274 T15270 compound Roche,GmbH
R9236 T15275 T15270 compound Diagnostics,GmbH
R9237 T15276 T15270 punct ", ",GmbH
R9238 T15277 T15270 npadvmod Mannheim,GmbH
R9239 T15278 T15270 punct ", ",GmbH
R9240 T15279 T15270 npadvmod Germany,GmbH
R9241 T15280 T15270 punct ),GmbH
R9242 T15281 T15268 cc and,BamHI
R9243 T15282 T15268 conj ScaI,BamHI
R9244 T15283 T15284 punct (,Roche
R9245 T15284 T15282 parataxis Roche,ScaI
R9246 T15285 T15286 nummod 30,U
R9247 T15286 T15284 dep U,Roche
R9248 T15287 T15284 punct ;,Roche
R9249 T15288 T15284 punct ),Roche
R9250 T15289 T15254 punct ", ",digested
R9251 T15290 T15254 conj fractionated,digested
R9252 T15291 T15290 prep on,fractionated
R9253 T15292 T15293 det a,gel
R9254 T15293 T15291 pobj gel,on
R9255 T15294 T15295 nummod 0.8,%
R9256 T15295 T15296 compound %,agarose
R9257 T15296 T15293 compound agarose,gel
R9258 T15297 T15290 punct ", ",fractionated
R9259 T15298 T15290 conj transferred,fractionated
R9260 T15299 T15298 prep to,transferred
R9261 T15300 T15301 det a,membrane
R9262 T15301 T15299 pobj membrane,to
R9263 T15302 T15301 compound nylon,membrane
R9264 T15303 T15304 punct (,GmbH
R9265 T15304 T15301 parataxis GmbH,membrane
R9266 T15305 T15306 compound Hybond,N
R9267 T15306 T15304 dep N,GmbH
R9268 T15307 T15304 punct ;,GmbH
R9269 T15308 T15304 compound Amersham,GmbH
R9270 T15309 T15304 compound Biosciences,GmbH
R9271 T15310 T15304 compound Europe,GmbH
R9272 T15311 T15304 punct ", ",GmbH
R9273 T15312 T15304 npadvmod Freiburg,GmbH
R9274 T15313 T15304 punct ", ",GmbH
R9275 T15314 T15304 npadvmod Germany,GmbH
R9276 T15315 T15304 punct ),GmbH
R9277 T15316 T15298 cc and,transferred
R9278 T15317 T15298 conj hybridized,transferred
R9279 T15318 T15317 prep with,hybridized
R9280 T15319 T15320 nummod 5,probes
R9281 T15320 T15318 pobj probes,with
R9282 T15321 T15319 punct ',5
R9283 T15322 T15319 cc and,5
R9284 T15323 T15319 conj 3,5
R9285 T15324 T15323 punct ',3
R9286 T15325 T15320 compound flanking,probes
R9287 T15326 T15254 punct .,digested
R9288 T15328 T15329 det The,digest
R9289 T15329 T15331 nsubjpass digest,hybridized
R9290 T15330 T15329 compound BamHI,digest
R9291 T15332 T15331 auxpass was,hybridized
R9292 T15333 T15331 prep with,hybridized
R9293 T15334 T15335 det a,probe
R9294 T15335 T15333 pobj probe,with
R9295 T15336 T15337 npadvmod Ptdsr,specific
R9296 T15337 T15335 amod specific,probe
R9297 T15338 T15337 punct -,specific
R9298 T15339 T15335 nummod 5,probe
R9299 T15340 T15339 punct ',5
R9300 T15341 T15335 compound flanking,probe
R9301 T15342 T15331 punct ", ",hybridized
R9302 T15343 T15331 cc and,hybridized
R9303 T15344 T15345 compound Southern,blot
R9304 T15345 T15346 nsubj blot,gave
R9305 T15346 T15331 conj gave,hybridized
R9306 T15347 T15348 det a,band
R9307 T15348 T15346 dobj band,gave
R9308 T15349 T15348 amod single,band
R9309 T15350 T15351 nummod 17.2,kb
R9310 T15351 T15348 compound kb,band
R9311 T15352 T15348 prep for,band
R9312 T15353 T15354 amod wild,type
R9313 T15354 T15352 pobj type,for
R9314 T15355 T15354 punct -,type
R9315 T15356 T15357 punct (,+
R9316 T15357 T15354 punct +,type
R9317 T15358 T15357 punct +,+
R9318 T15359 T15357 punct /,+
R9319 T15360 T15357 punct ),+
R9320 T15361 T15348 punct ", ",band
R9321 T15362 T15363 det an,band
R9322 T15363 T15348 conj band,band
R9323 T15364 T15365 nummod 11.6,kb
R9324 T15365 T15363 compound kb,band
R9325 T15366 T15363 prep for,band
R9326 T15367 T15366 pobj homozygous,for
R9327 T15368 T15369 punct (,-
R9328 T15369 T15367 punct -,homozygous
R9329 T15370 T15369 punct -,-
R9330 T15371 T15369 punct /,-
R9331 T15372 T15369 punct ),-
R9332 T15373 T15363 cc and,band
R9333 T15374 T15375 det both,bands
R9334 T15375 T15376 nmod bands,mice
R9335 T15376 T15363 conj mice,band
R9336 T15377 T15375 prep for,bands
R9337 T15378 T15377 pobj heterozygous,for
R9338 T15379 T15380 punct (,-
R9339 T15380 T15378 punct -,heterozygous
R9340 T15381 T15380 punct +,-
R9341 T15382 T15380 punct /,-
R9342 T15383 T15380 punct ),-
R9343 T15384 T15346 punct .,gave
R9344 T15386 T15387 det The,digest
R9345 T15387 T15389 nsubjpass digest,hybridized
R9346 T15388 T15387 compound ScaI,digest
R9347 T15390 T15389 auxpass was,hybridized
R9348 T15391 T15389 advcl using,hybridized
R9349 T15392 T15393 det a,probe
R9350 T15393 T15391 dobj probe,using
R9351 T15394 T15393 nummod 3,probe
R9352 T15395 T15394 punct ',3
R9353 T15396 T15393 compound flanking,probe
R9354 T15397 T15389 punct ", ",hybridized
R9355 T15398 T15389 cc and,hybridized
R9356 T15399 T15400 compound Southern,blot
R9357 T15400 T15401 nsubj blot,gave
R9358 T15401 T15389 conj gave,hybridized
R9359 T15402 T15403 det a,band
R9360 T15403 T15401 dobj band,gave
R9361 T15404 T15403 amod single,band
R9362 T15405 T15406 nummod 12.4,kb
R9363 T15406 T15403 compound kb,band
R9364 T15407 T15401 prep for,gave
R9365 T15408 T15409 amod wild,type
R9366 T15409 T15407 pobj type,for
R9367 T15410 T15409 punct -,type
R9368 T15411 T15401 punct ", ",gave
R9369 T15412 T15413 det a,band
R9370 T15413 T15401 conj band,gave
R9371 T15414 T15415 nummod 17.2,kb
R9372 T15415 T15413 compound kb,band
R9373 T15416 T15413 prep for,band
R9374 T15417 T15416 pobj homozygous,for
R9375 T15418 T15413 cc and,band
R9376 T15419 T15420 det both,bands
R9377 T15420 T15413 conj bands,band
R9378 T15421 T15420 prep for,bands
R9379 T15422 T15421 pobj heterozygous,for
R9380 T15423 T15420 dobj mice,bands
R9381 T15424 T15401 punct .,gave
R9382 T15486 T15487 compound Northern,blot
R9383 T15487 T15488 compound blot,analysis
R9384 T15490 T15491 amod Total,RNA
R9385 T15491 T15492 nsubjpass RNA,isolated
R9386 T15493 T15492 auxpass was,isolated
R9387 T15494 T15492 prep from,isolated
R9388 T15495 T15496 amod homogenized,embryos
R9389 T15496 T15494 pobj embryos,from
R9390 T15497 T15492 advcl using,isolated
R9391 T15498 T15499 compound TRIZOL,reagent
R9392 T15499 T15497 dobj reagent,using
R9393 T15500 T15501 punct (,GmbH
R9394 T15501 T15499 parataxis GmbH,reagent
R9395 T15502 T15501 compound Invitrogen,GmbH
R9396 T15503 T15501 punct ", ",GmbH
R9397 T15504 T15501 npadvmod Karlsruhe,GmbH
R9398 T15505 T15501 punct ", ",GmbH
R9399 T15506 T15501 npadvmod Germany,GmbH
R9400 T15507 T15501 punct ),GmbH
R9401 T15508 T15492 punct .,isolated
R9402 T15510 T15511 prep For,extracted
R9403 T15512 T15513 compound northern,blots
R9404 T15513 T15510 pobj blots,For
R9405 T15514 T15511 punct ", ",extracted
R9406 T15515 T15511 preconj either,extracted
R9407 T15516 T15517 amod total,RNA
R9408 T15517 T15511 nsubjpass RNA,extracted
R9409 T15518 T15519 punct (,μg
R9410 T15519 T15517 parataxis μg,RNA
R9411 T15520 T15519 nummod 30,μg
R9412 T15521 T15519 punct ),μg
R9413 T15522 T15511 auxpass was,extracted
R9414 T15523 T15511 prep from,extracted
R9415 T15524 T15523 pobj embryos,from
R9416 T15525 T15511 punct ", ",extracted
R9417 T15526 T15511 conj electrophoresed,extracted
R9418 T15527 T15526 cc and,electrophoresed
R9419 T15528 T15526 conj transferred,electrophoresed
R9420 T15529 T15528 prep to,transferred
R9421 T15530 T15531 det a,membrane
R9422 T15531 T15529 pobj membrane,to
R9423 T15532 T15531 compound nylon,membrane
R9424 T15533 T15534 punct (,N
R9425 T15534 T15531 parataxis N,membrane
R9426 T15535 T15534 compound Hybond,N
R9427 T15536 T15534 punct ;,N
R9428 T15537 T15534 npadvmod Amersham,N
R9429 T15538 T15534 punct ),N
R9430 T15539 T15511 cc or,extracted
R9431 T15540 T15541 det a,blot
R9432 T15541 T15545 nsubjpass blot,hybridized
R9433 T15542 T15541 compound polyA+,blot
R9434 T15543 T15541 compound RNA,blot
R9435 T15544 T15541 compound northern,blot
R9436 T15545 T15511 conj hybridized,extracted
R9437 T15546 T15547 punct (,Inc.
R9438 T15547 T15541 parataxis Inc.,blot
R9439 T15548 T15547 compound OriGene,Inc.
R9440 T15549 T15547 compound Technologies,Inc.
R9441 T15550 T15547 punct ", ",Inc.
R9442 T15551 T15547 npadvmod Rockville,Inc.
R9443 T15552 T15547 punct ", ",Inc.
R9444 T15553 T15547 npadvmod USA,Inc.
R9445 T15554 T15547 punct ),Inc.
R9446 T15555 T15545 auxpass was,hybridized
R9447 T15556 T15545 advcl using,hybridized
R9448 T15557 T15556 prep as,using
R9449 T15558 T15559 det the,probe
R9450 T15559 T15557 pobj probe,as
R9451 T15560 T15561 det a,fragment
R9452 T15561 T15556 dobj fragment,using
R9453 T15562 T15561 compound Ptdsr,fragment
R9454 T15563 T15561 acl amplified,fragment
R9455 T15564 T15563 prep from,amplified
R9456 T15565 T15566 amod wild,type
R9457 T15566 T15568 compound type,cDNA
R9458 T15567 T15566 punct -,type
R9459 T15568 T15564 pobj cDNA,from
R9460 T15569 T15563 advcl using,amplified
R9461 T15570 T15571 amod forward,primer
R9462 T15571 T15572 nmod primer,GTTCCAGCTCGTCAGACTCG
R9463 T15572 T15569 dobj GTTCCAGCTCGTCAGACTCG,using
R9464 T15573 T15572 nummod 5,GTTCCAGCTCGTCAGACTCG
R9465 T15574 T15573 punct ',5
R9466 T15575 T15572 punct -,GTTCCAGCTCGTCAGACTCG
R9467 T15576 T15572 punct -,GTTCCAGCTCGTCAGACTCG
R9468 T15577 T15572 nummod 3,GTTCCAGCTCGTCAGACTCG
R9469 T15578 T15572 punct ',GTTCCAGCTCGTCAGACTCG
R9470 T15579 T15572 cc and,GTTCCAGCTCGTCAGACTCG
R9471 T15580 T15581 amod reverse,primer
R9472 T15581 T15582 nmod primer,TGCCCCTAAGACATGACCAC
R9473 T15582 T15572 conj TGCCCCTAAGACATGACCAC,GTTCCAGCTCGTCAGACTCG
R9474 T15583 T15582 nummod 5,TGCCCCTAAGACATGACCAC
R9475 T15584 T15583 punct ',5
R9476 T15585 T15582 punct -,TGCCCCTAAGACATGACCAC
R9477 T15586 T15582 punct -,TGCCCCTAAGACATGACCAC
R9478 T15587 T15582 nummod 3,TGCCCCTAAGACATGACCAC
R9479 T15588 T15582 punct ',TGCCCCTAAGACATGACCAC
R9480 T15589 T15545 punct .,hybridized
R9481 T15591 T15592 prep In,re-hybridized
R9482 T15593 T15594 det all,experiments
R9483 T15594 T15591 pobj experiments,In
R9484 T15595 T15596 det the,membrane
R9485 T15596 T15592 nsubjpass membrane,re-hybridized
R9486 T15597 T15596 amod same,membrane
R9487 T15598 T15592 auxpass was,re-hybridized
R9488 T15599 T15592 prep with,re-hybridized
R9489 T15600 T15601 det a,probe
R9490 T15601 T15599 pobj probe,with
R9491 T15602 T15603 compound β,actin
R9492 T15603 T15601 compound actin,probe
R9493 T15604 T15603 punct -,actin
R9494 T15605 T15606 punct (,OriGene
R9495 T15606 T15601 parataxis OriGene,probe
R9496 T15607 T15606 punct ),OriGene
R9497 T15608 T15609 aux to,confirm
R9498 T15609 T15592 advcl confirm,re-hybridized
R9499 T15610 T15611 mark that,were
R9500 T15611 T15609 ccomp were,confirm
R9501 T15612 T15613 amod equivalent,levels
R9502 T15613 T15611 nsubj levels,were
R9503 T15614 T15613 compound RNA,levels
R9504 T15615 T15611 acomp present,were
R9505 T15616 T15611 prep in,were
R9506 T15617 T15618 det each,lane
R9507 T15618 T15616 pobj lane,in
R9508 T15619 T15592 punct .,re-hybridized
R9509 T15621 T15622 compound Northern,blotting
R9510 T15622 T15623 nsubj blotting,indicated
R9511 T15624 T15625 mark that,express
R9512 T15625 T15623 ccomp express,indicated
R9513 T15626 T15627 amod homozygous,embryos
R9514 T15627 T15625 nsubj embryos,express
R9515 T15628 T15627 amod mutant,embryos
R9516 T15629 T15625 aux did,express
R9517 T15630 T15625 neg not,express
R9518 T15631 T15632 compound Ptdsr,mRNA
R9519 T15632 T15625 dobj mRNA,express
R9520 T15633 T15625 cc and,express
R9521 T15634 T15635 amod heterozygous,embryos
R9522 T15635 T15637 nsubj embryos,expressed
R9523 T15636 T15635 compound mutant,embryos
R9524 T15637 T15625 conj expressed,express
R9525 T15638 T15639 advmod only,amounts
R9526 T15639 T15637 dobj amounts,expressed
R9527 T15640 T15639 amod reduced,amounts
R9528 T15641 T15639 prep of,amounts
R9529 T15642 T15643 compound Ptdsr,mRNA
R9530 T15643 T15641 pobj mRNA,of
R9531 T15644 T15623 punct .,indicated
R9532 T15701 T15702 compound Western,blot
R9533 T15702 T15703 compound blot,analysis
R9534 T15705 T15706 nsubjpass Embryos,homogenized
R9535 T15707 T15708 punct (,E13.5
R9536 T15708 T15705 parataxis E13.5,Embryos
R9537 T15709 T15708 punct ),E13.5
R9538 T15710 T15705 prep for,Embryos
R9539 T15711 T15712 compound protein,isolation
R9540 T15712 T15710 pobj isolation,for
R9541 T15713 T15706 auxpass were,homogenized
R9542 T15714 T15706 prep in,homogenized
R9543 T15715 T15716 compound lysis,buffer
R9544 T15716 T15714 pobj buffer,in
R9545 T15717 T15716 acl containing,buffer
R9546 T15718 T15719 nummod 1,PBS
R9547 T15719 T15717 dobj PBS,containing
R9548 T15720 T15718 punct ×,1
R9549 T15721 T15719 punct ", ",PBS
R9550 T15722 T15723 nummod 1,%
R9551 T15723 T15724 compound %,40
R9552 T15724 T15719 conj 40,PBS
R9553 T15725 T15724 compound Nonidet,40
R9554 T15726 T15724 compound P,40
R9555 T15727 T15724 punct -,40
R9556 T15728 T15724 punct ", ",40
R9557 T15729 T15730 nummod 0.5,%
R9558 T15730 T15731 compound %,deoxycholate
R9559 T15731 T15724 conj deoxycholate,40
R9560 T15732 T15731 compound sodium,deoxycholate
R9561 T15733 T15731 punct ", ",deoxycholate
R9562 T15734 T15735 nummod 0.1,%
R9563 T15735 T15736 compound %,SDS
R9564 T15736 T15731 conj SDS,deoxycholate
R9565 T15737 T15736 cc and,SDS
R9566 T15738 T15739 compound protease,cocktail
R9567 T15739 T15736 conj cocktail,SDS
R9568 T15740 T15739 compound inhibitor,cocktail
R9569 T15741 T15742 punct (,Roche
R9570 T15742 T15739 parataxis Roche,cocktail
R9571 T15743 T15742 dep CompleteMini,Roche
R9572 T15744 T15742 punct ;,Roche
R9573 T15745 T15742 punct ),Roche
R9574 T15746 T15706 punct .,homogenized
R9575 T15748 T15749 amod Equal,amounts
R9576 T15749 T15750 nsubjpass amounts,separated
R9577 T15751 T15752 punct (,μg
R9578 T15752 T15749 parataxis μg,amounts
R9579 T15753 T15752 nummod 25,μg
R9580 T15754 T15752 punct ),μg
R9581 T15755 T15749 prep of,amounts
R9582 T15756 T15757 compound protein,lysate
R9583 T15757 T15755 pobj lysate,of
R9584 T15758 T15750 auxpass were,separated
R9585 T15759 T15750 prep by,separated
R9586 T15760 T15761 compound SDS,polyacrylamide
R9587 T15761 T15763 compound polyacrylamide,electrophoresis
R9588 T15762 T15761 punct -,polyacrylamide
R9589 T15763 T15759 pobj electrophoresis,by
R9590 T15764 T15763 compound gel,electrophoresis
R9591 T15765 T15750 cc and,separated
R9592 T15766 T15750 conj transferred,separated
R9593 T15767 T15766 prep onto,transferred
R9594 T15768 T15769 det a,membrane
R9595 T15769 T15767 pobj membrane,onto
R9596 T15770 T15769 compound PVDF,membrane
R9597 T15771 T15772 punct (,Millipore
R9598 T15772 T15769 parataxis Millipore,membrane
R9599 T15773 T15772 punct ", ",Millipore
R9600 T15774 T15772 npadvmod Billerica,Millipore
R9601 T15775 T15772 punct ", ",Millipore
R9602 T15776 T15772 npadvmod USA,Millipore
R9603 T15777 T15772 punct ),Millipore
R9604 T15778 T15766 prep according,transferred
R9605 T15779 T15778 prep to,according
R9606 T15780 T15781 amod standard,protocols
R9607 T15781 T15779 pobj protocols,to
R9608 T15782 T15750 punct .,separated
R9609 T15784 T15785 compound Western,blots
R9610 T15785 T15786 nsubjpass blots,done
R9611 T15787 T15786 auxpass were,done
R9612 T15788 T15786 advcl using,done
R9613 T15789 T15790 det a,antibody
R9614 T15790 T15788 dobj antibody,using
R9615 T15791 T15790 amod specific,antibody
R9616 T15792 T15790 prep to,antibody
R9617 T15793 T15792 pobj Ptdsr,to
R9618 T15794 T15795 punct (,Inc.
R9619 T15795 T15790 parataxis Inc.,antibody
R9620 T15796 T15797 compound PSR,N
R9621 T15797 T15795 dep N,Inc.
R9622 T15798 T15797 punct -,N
R9623 T15799 T15797 nummod 20,N
R9624 T15800 T15795 punct ", ",Inc.
R9625 T15801 T15795 dep sc,Inc.
R9626 T15802 T15801 punct -,sc
R9627 T15803 T15801 nummod 11632,sc
R9628 T15804 T15795 punct ;,Inc.
R9629 T15805 T15795 compound Santa,Inc.
R9630 T15806 T15795 compound Cruz,Inc.
R9631 T15807 T15795 compound Biotechnology,Inc.
R9632 T15808 T15795 punct ", ",Inc.
R9633 T15809 T15810 compound Santa,Cruz
R9634 T15810 T15795 npadvmod Cruz,Inc.
R9635 T15811 T15795 punct ", ",Inc.
R9636 T15812 T15795 npadvmod USA,Inc.
R9637 T15813 T15795 punct ),Inc.
R9638 T15814 T15790 cc and,antibody
R9639 T15815 T15816 compound β,actin
R9640 T15816 T15790 conj actin,antibody
R9641 T15817 T15816 punct -,actin
R9642 T15818 T15819 punct (,Abcam
R9643 T15819 T15816 parataxis Abcam,actin
R9644 T15820 T15819 dep ab,Abcam
R9645 T15821 T15820 punct -,ab
R9646 T15822 T15820 nummod 6276,ab
R9647 T15823 T15819 punct ;,Abcam
R9648 T15824 T15819 punct ", ",Abcam
R9649 T15825 T15819 npadvmod Cambridge,Abcam
R9650 T15826 T15819 punct ", ",Abcam
R9651 T15827 T15819 npadvmod UK,Abcam
R9652 T15828 T15819 punct ),Abcam
R9653 T15829 T15830 mark as,described
R9654 T15830 T15786 advcl described,done
R9655 T15831 T15830 agent by,described
R9656 T15832 T15833 det the,supplier
R9657 T15833 T15831 pobj supplier,by
R9658 T15834 T15786 punct .,done
R9659 T15836 T15837 amod Secondary,antibodies
R9660 T15837 T15838 nsubj antibodies,were
R9661 T15839 T15837 acl conjugated,antibodies
R9662 T15840 T15839 prep to,conjugated
R9663 T15841 T15842 compound horseradish,peroxidase
R9664 T15842 T15840 pobj peroxidase,to
R9665 T15843 T15838 prep from,were
R9666 T15844 T15845 compound Santa,Cruz
R9667 T15845 T15843 pobj Cruz,from
R9668 T15846 T15845 cc and,Cruz
R9669 T15847 T15845 conj Abcam,Cruz
R9670 T15848 T15838 punct ", ",were
R9671 T15849 T15838 advcl used,were
R9672 T15850 T15851 mark as,described
R9673 T15851 T15849 advcl described,used
R9674 T15852 T15851 agent by,described
R9675 T15853 T15854 det the,supplier
R9676 T15854 T15852 pobj supplier,by
R9677 T15855 T15838 punct ", ",were
R9678 T15856 T15838 cc and,were
R9679 T15857 T15858 nsubjpass detection,performed
R9680 T15858 T15838 conj performed,were
R9681 T15859 T15858 auxpass was,performed
R9682 T15860 T15858 prep with,performed
R9683 T15861 T15862 det an,system
R9684 T15862 T15860 pobj system,with
R9685 T15863 T15862 amod enhanced,system
R9686 T15864 T15862 compound chemiluminescence,system
R9687 T15865 T15866 punct (,ECLPlus
R9688 T15866 T15862 parataxis ECLPlus,system
R9689 T15867 T15866 punct ;,ECLPlus
R9690 T15868 T15866 npadvmod Amersham,ECLPlus
R9691 T15869 T15866 punct ),ECLPlus
R9692 T15870 T15858 punct .,performed
R9693 T15896 T15897 compound Animal,experiments
R9694 T15899 T15900 amod Wild,type
R9695 T15900 T15902 nmod type,mice
R9696 T15901 T15900 punct -,type
R9697 T15902 T15910 nsubjpass mice,obtained
R9698 T15903 T15904 nmod C57BL,6J
R9699 T15904 T15902 nmod 6J,mice
R9700 T15905 T15904 punct /,6J
R9701 T15906 T15904 cc and,6J
R9702 T15907 T15908 compound 129P2,OlaHsd
R9703 T15908 T15904 conj OlaHsd,6J
R9704 T15909 T15908 punct /,OlaHsd
R9705 T15911 T15910 auxpass were,obtained
R9706 T15912 T15910 prep from,obtained
R9707 T15913 T15914 compound Jackson,Laboratories
R9708 T15914 T15912 pobj Laboratories,from
R9709 T15915 T15916 punct (,Harbor
R9710 T15916 T15914 parataxis Harbor,Laboratories
R9711 T15917 T15916 compound Bar,Harbor
R9712 T15918 T15916 punct ", ",Harbor
R9713 T15919 T15916 npadvmod USA,Harbor
R9714 T15920 T15916 punct ),Harbor
R9715 T15921 T15914 cc and,Laboratories
R9716 T15922 T15923 compound Harlan,UK
R9717 T15923 T15914 conj UK,Laboratories
R9718 T15924 T15925 punct (,Bicester
R9719 T15925 T15923 parataxis Bicester,UK
R9720 T15926 T15925 punct ", ",Bicester
R9721 T15927 T15925 npadvmod UK,Bicester
R9722 T15928 T15925 punct ),Bicester
R9723 T15929 T15910 punct ", ",obtained
R9724 T15930 T15910 advmod respectively,obtained
R9725 T15931 T15910 punct .,obtained
R9726 T15933 T15934 det All,mice
R9727 T15934 T15935 nsubjpass mice,housed
R9728 T15936 T15935 auxpass were,housed
R9729 T15937 T15935 prep in,housed
R9730 T15938 T15939 advmod individually,ventilated
R9731 T15939 T15940 amod ventilated,cages
R9732 T15940 T15937 pobj cages,in
R9733 T15941 T15935 prep in,housed
R9734 T15942 T15943 det a,environment
R9735 T15943 T15941 pobj environment,in
R9736 T15944 T15943 amod specific,environment
R9737 T15945 T15946 npadvmod pathogen,free
R9738 T15946 T15943 amod free,environment
R9739 T15947 T15946 punct -,free
R9740 T15948 T15935 prep with,housed
R9741 T15949 T15950 det a,cycle
R9742 T15950 T15948 pobj cycle,with
R9743 T15951 T15952 nummod 12,h
R9744 T15952 T15950 nmod h,cycle
R9745 T15953 T15954 amod light,dark
R9746 T15954 T15950 nmod dark,cycle
R9747 T15955 T15954 punct -,dark
R9748 T15956 T15935 cc and,housed
R9749 T15957 T15958 auxpass were,fed
R9750 T15958 T15935 conj fed,housed
R9751 T15959 T15960 det a,diet
R9752 T15960 T15958 dobj diet,fed
R9753 T15961 T15960 amod regular,diet
R9754 T15962 T15960 amod unrestricted,diet
R9755 T15963 T15935 punct .,housed
R9756 T15965 T15966 det The,GBF
R9757 T15966 T15967 poss GBF,program
R9758 T15967 T15971 nsubj program,screened
R9759 T15968 T15966 case 's,GBF
R9760 T15969 T15967 amod routine,program
R9761 T15970 T15967 compound surveillance,program
R9762 T15972 T15971 prep for,screened
R9763 T15973 T15974 amod selected,pathogens
R9764 T15974 T15972 pobj pathogens,for
R9765 T15975 T15971 punct .,screened
R9766 T15977 T15978 det The,mutant
R9767 T15978 T15980 nsubjpass mutant,crossed
R9768 T15979 T15978 compound Ptdsrtm1Gbf,mutant
R9769 T15981 T15980 auxpass was,crossed
R9770 T15982 T15980 prep to,crossed
R9771 T15983 T15984 compound C57BL,6J
R9772 T15984 T15986 compound 6J,mice
R9773 T15985 T15984 punct /,6J
R9774 T15986 T15982 pobj mice,to
R9775 T15987 T15988 aux to,establish
R9776 T15988 T15980 advcl establish,crossed
R9777 T15989 T15990 det the,line
R9778 T15990 T15988 dobj line,establish
R9779 T15991 T15990 amod co-isogenic,line
R9780 T15992 T15993 compound C57BL,6J
R9781 T15993 T15995 compound 6J,Ptdsrtm1Gbf
R9782 T15994 T15993 punct /,6J
R9783 T15995 T15990 compound Ptdsrtm1Gbf,line
R9784 T15996 T15995 punct -,Ptdsrtm1Gbf
R9785 T15997 T15990 compound mouse,line
R9786 T15998 T15980 punct .,crossed
R9787 T16000 T16001 det All,studies
R9788 T16001 T16002 nsubjpass studies,approved
R9789 T16003 T16002 auxpass were,approved
R9790 T16004 T16002 agent by,approved
R9791 T16005 T16006 det the,authorities
R9792 T16006 T16004 pobj authorities,by
R9793 T16007 T16006 amod appropriate,authorities
R9794 T16008 T16002 punct .,approved
R9795 T16040 T16039 prep of,Isolation
R9796 T16041 T16040 pobj embryos,of
R9797 T16043 T16044 amod Heterozygous,mice
R9798 T16044 T16048 nsubjpass mice,intercrossed
R9799 T16045 T16044 amod male,mice
R9800 T16046 T16045 cc and,male
R9801 T16047 T16045 conj female,male
R9802 T16049 T16048 auxpass were,intercrossed
R9803 T16050 T16048 prep in,intercrossed
R9804 T16051 T16050 pobj order,in
R9805 T16052 T16053 aux to,obtain
R9806 T16053 T16051 acl obtain,order
R9807 T16054 T16055 npadvmod Ptdsr,deficient
R9808 T16055 T16057 amod deficient,progeny
R9809 T16056 T16055 punct -,deficient
R9810 T16057 T16053 dobj progeny,obtain
R9811 T16058 T16048 punct .,intercrossed
R9812 T16060 T16061 nsubjpass Females,monitored
R9813 T16062 T16061 auxpass were,monitored
R9814 T16063 T16061 advmod daily,monitored
R9815 T16064 T16061 prep for,monitored
R9816 T16065 T16066 amod vaginal,plugs
R9817 T16066 T16064 pobj plugs,for
R9818 T16067 T16061 punct ", ",monitored
R9819 T16068 T16061 cc and,monitored
R9820 T16069 T16070 nsubjpass noon,defined
R9821 T16070 T16061 conj defined,monitored
R9822 T16071 T16069 prep of,noon
R9823 T16072 T16073 det the,day
R9824 T16073 T16071 pobj day,of
R9825 T16074 T16073 prep of,day
R9826 T16075 T16076 compound plug,detection
R9827 T16076 T16074 pobj detection,of
R9828 T16077 T16070 auxpass was,defined
R9829 T16078 T16070 prep as,defined
R9830 T16079 T16078 pobj E0.5,as
R9831 T16080 T16070 punct .,defined
R9832 T16082 T16083 nsubjpass Embryos,dissected
R9833 T16084 T16082 prep at,Embryos
R9834 T16085 T16086 amod indicated,points
R9835 T16086 T16084 pobj points,at
R9836 T16087 T16086 compound time,points
R9837 T16088 T16083 auxpass were,dissected
R9838 T16089 T16083 prep in,dissected
R9839 T16090 T16091 amod sterile,PBS
R9840 T16091 T16089 pobj PBS,in
R9841 T16092 T16083 punct ", ",dissected
R9842 T16093 T16083 conj washed,dissected
R9843 T16094 T16093 prep in,washed
R9844 T16095 T16096 npadvmod ice,cold
R9845 T16096 T16098 amod cold,PBS
R9846 T16097 T16096 punct -,cold
R9847 T16098 T16094 pobj PBS,in
R9848 T16099 T16093 cc and,washed
R9849 T16100 T16093 conj transferred,washed
R9850 T16101 T16100 prep to,transferred
R9851 T16102 T16103 amod cold,fixative
R9852 T16103 T16101 pobj fixative,to
R9853 T16104 T16083 punct .,dissected
R9854 T16106 T16107 amod Extra-embryonic,membranes
R9855 T16107 T16108 nsubjpass membranes,kept
R9856 T16109 T16108 auxpass were,kept
R9857 T16110 T16108 cc and,kept
R9858 T16111 T16108 conj used,kept
R9859 T16112 T16111 prep for,used
R9860 T16113 T16112 pobj genotyping,for
R9861 T16114 T16108 punct .,kept
R9862 T16116 T16117 nmod Ptdsr,embryos
R9863 T16117 T16121 nsubjpass embryos,used
R9864 T16118 T16116 punct -,Ptdsr
R9865 T16119 T16116 punct /,Ptdsr
R9866 T16120 T16116 punct -,Ptdsr
R9867 T16122 T16117 cc and,embryos
R9868 T16123 T16124 poss their,littermates
R9869 T16124 T16117 conj littermates,embryos
R9870 T16125 T16126 amod wild,type
R9871 T16126 T16124 compound type,littermates
R9872 T16127 T16126 punct -,type
R9873 T16128 T16121 auxpass were,used
R9874 T16129 T16121 prep for,used
R9875 T16130 T16129 pobj experiments,for
R9876 T16131 T16121 punct .,used
R9877 T16204 T16203 punct ", ",Histology
R9878 T16205 T16206 compound TUNEL,staining
R9879 T16206 T16203 conj staining,Histology
R9880 T16207 T16206 cc and,staining
R9881 T16208 T16206 conj immunohistochemistry,staining
R9882 T16210 T16211 nsubjpass Embryos,harvested
R9883 T16212 T16210 prep for,Embryos
R9884 T16213 T16212 pobj histology,for
R9885 T16214 T16213 cc and,histology
R9886 T16215 T16213 conj immunohistochemistry,histology
R9887 T16216 T16211 auxpass were,harvested
R9888 T16217 T16211 cc and,harvested
R9889 T16218 T16211 conj fixed,harvested
R9890 T16219 T16218 prep in,fixed
R9891 T16220 T16221 nummod 10,%
R9892 T16221 T16222 nmod %,formalin
R9893 T16222 T16219 pobj formalin,in
R9894 T16223 T16224 advmod neutral,buffered
R9895 T16224 T16222 amod buffered,formalin
R9896 T16225 T16224 punct -,buffered
R9897 T16226 T16211 punct ", ",harvested
R9898 T16227 T16211 dep dehydrated,harvested
R9899 T16228 T16227 prep through,dehydrated
R9900 T16229 T16230 det a,series
R9901 T16230 T16228 pobj series,through
R9902 T16231 T16230 amod graded,series
R9903 T16232 T16230 prep of,series
R9904 T16233 T16232 pobj alcohol,of
R9905 T16234 T16211 punct ", ",harvested
R9906 T16235 T16211 dep embedded,harvested
R9907 T16236 T16235 prep in,embedded
R9908 T16237 T16236 pobj paraffin,in
R9909 T16238 T16211 punct ", ",harvested
R9910 T16239 T16240 advmod sagittally,sectioned
R9911 T16240 T16211 dep sectioned,harvested
R9912 T16241 T16240 prep at,sectioned
R9913 T16242 T16243 nummod 5,μm
R9914 T16243 T16244 compound μm,intervals
R9915 T16244 T16241 pobj intervals,at
R9916 T16245 T16211 punct ", ",harvested
R9917 T16246 T16211 cc and,harvested
R9918 T16247 T16248 det every,section
R9919 T16248 T16250 nsubjpass section,processed
R9920 T16249 T16248 amod fifth,section
R9921 T16250 T16211 conj processed,harvested
R9922 T16251 T16250 auxpass was,processed
R9923 T16252 T16250 prep for,processed
R9924 T16253 T16254 nmod hematoxylin,staining
R9925 T16254 T16252 pobj staining,for
R9926 T16255 T16253 cc and,hematoxylin
R9927 T16256 T16253 conj eosin,hematoxylin
R9928 T16257 T16253 punct (,hematoxylin
R9929 T16258 T16253 appos H,hematoxylin
R9930 T16259 T16258 cc &,H
R9931 T16260 T16258 conj E,H
R9932 T16261 T16253 punct ),hematoxylin
R9933 T16262 T16250 prep according,processed
R9934 T16263 T16262 prep to,according
R9935 T16264 T16265 amod standard,protocols
R9936 T16265 T16263 pobj protocols,to
R9937 T16266 T16250 punct .,processed
R9938 T16268 T16269 amod Remaining,sections
R9939 T16269 T16270 nsubjpass sections,used
R9940 T16271 T16269 prep of,sections
R9941 T16272 T16273 amod wild,type
R9942 T16273 T16275 nmod type,specimens
R9943 T16274 T16273 punct -,type
R9944 T16275 T16271 pobj specimens,of
R9945 T16276 T16273 cc and,type
R9946 T16277 T16273 conj Ptdsr,type
R9947 T16278 T16277 punct -,Ptdsr
R9948 T16279 T16277 punct /,Ptdsr
R9949 T16280 T16277 punct -,Ptdsr
R9950 T16281 T16270 auxpass were,used
R9951 T16282 T16270 prep for,used
R9952 T16283 T16282 pobj immunohistochemistry,for
R9953 T16284 T16270 punct .,used
R9954 T16286 T16287 prep For,used
R9955 T16288 T16286 pobj detection,For
R9956 T16289 T16288 prep of,detection
R9957 T16290 T16291 amod apoptotic,cells
R9958 T16291 T16289 pobj cells,of
R9959 T16292 T16291 cc and,cells
R9960 T16293 T16291 conj macrophages,cells
R9961 T16294 T16287 punct ", ",used
R9962 T16295 T16287 nsubjpass anti-aCasp3,used
R9963 T16296 T16297 punct (,Systems
R9964 T16297 T16295 parataxis Systems,anti-aCasp3
R9965 T16298 T16299 det an,antibody
R9966 T16299 T16297 dep antibody,Systems
R9967 T16300 T16299 amod specific,antibody
R9968 T16301 T16300 prep for,specific
R9969 T16302 T16303 amod activated,caspase
R9970 T16303 T16301 pobj caspase,for
R9971 T16304 T16303 nummod 3,caspase
R9972 T16305 T16297 punct ;,Systems
R9973 T16306 T16297 nmod R,Systems
R9974 T16307 T16306 cc &,R
R9975 T16308 T16306 conj D,R
R9976 T16309 T16297 punct ", ",Systems
R9977 T16310 T16297 npadvmod Minneapolis,Systems
R9978 T16311 T16297 punct ", ",Systems
R9979 T16312 T16297 npadvmod USA,Systems
R9980 T16313 T16297 punct ),Systems
R9981 T16314 T16295 cc and,anti-aCasp3
R9982 T16315 T16316 nmod anti-F4,antibodies
R9983 T16316 T16295 conj antibodies,anti-aCasp3
R9984 T16317 T16315 punct /,anti-F4
R9985 T16318 T16315 nummod 80,anti-F4
R9986 T16319 T16320 punct (,MCA
R9987 T16320 T16316 parataxis MCA,antibodies
R9988 T16321 T16322 compound Serotec,GmBH
R9989 T16322 T16320 dep GmBH,MCA
R9990 T16323 T16322 punct ", ",GmBH
R9991 T16324 T16322 npadvmod Düsseldorf,GmBH
R9992 T16325 T16322 punct ", ",GmBH
R9993 T16326 T16322 npadvmod Germany,GmBH
R9994 T16327 T16320 punct ;,MCA
R9995 T16328 T16320 compound #,MCA
R9996 T16329 T16320 nummod 1957,MCA
R9997 T16330 T16320 punct ),MCA
R9998 T16331 T16287 auxpass were,used
R9999 T16332 T16333 mark as,described