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PMC:539297 / 6145-11898 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1775 0-10 JJ denotes Homozygous
T1777 11-21 NN denotes CbCln3Δex7
T1778 21-22 HYPH denotes /
T1779 22-23 CD denotes 8
T1776 24-29 NNS denotes cells
T1780 30-37 VBP denotes express
T1781 38-44 NN denotes mutant
T1782 45-53 NN denotes battenin
T1783 54-57 CC denotes and
T1784 58-65 VBP denotes display
T1785 66-70 NN denotes JNCL
T1787 70-71 HYPH denotes -
T1786 71-75 JJ denotes like
T1788 76-85 NN denotes pathology
T1789 85-167 sentence denotes Homozygous Cb Cln3Δex7/8 cells were first examined for JNCL-like characteristics.
T1790 86-96 JJ denotes Homozygous
T1792 97-99 NN denotes Cb
T1793 100-108 NN denotes Cln3Δex7
T1794 108-109 HYPH denotes /
T1795 109-110 CD denotes 8
T1791 111-116 NNS denotes cells
T1797 117-121 VBD denotes were
T1798 122-127 RB denotes first
T1796 128-136 VBN denotes examined
T1799 137-140 IN denotes for
T1800 141-145 NN denotes JNCL
T1802 145-146 HYPH denotes -
T1801 146-150 JJ denotes like
T1803 151-166 NNS denotes characteristics
T1804 166-167 . denotes .
T1805 167-349 sentence denotes Homozygous Cln3Δex7/8 knock-in mice express multiple Cln3 mRNA splice variants and mutant battenin protein that is detectable by batp1 antibody recognizing C-terminal epitopes [12].
T1806 168-178 JJ denotes Homozygous
T1808 179-187 NN denotes Cln3Δex7
T1809 187-188 HYPH denotes /
T1810 188-189 CD denotes 8
T1811 190-195 VB denotes knock
T1812 195-196 HYPH denotes -
T1813 196-198 RP denotes in
T1807 199-203 NNS denotes mice
T1814 204-211 VBP denotes express
T1815 212-220 JJ denotes multiple
T1817 221-225 NN denotes Cln3
T1819 226-230 NN denotes mRNA
T1818 231-237 NN denotes splice
T1816 238-246 NNS denotes variants
T1820 247-250 CC denotes and
T1821 251-257 NN denotes mutant
T1823 258-266 NN denotes battenin
T1822 267-274 NN denotes protein
T1824 275-279 WDT denotes that
T1825 280-282 VBZ denotes is
T1826 283-293 JJ denotes detectable
T1827 294-296 IN denotes by
T1828 297-302 NN denotes batp1
T1829 303-311 NN denotes antibody
T1830 312-323 VBG denotes recognizing
T1832 324-325 NN denotes C
T1834 325-326 HYPH denotes -
T1833 326-334 JJ denotes terminal
T1831 335-343 NNS denotes epitopes
T1835 344-345 -LRB- denotes [
T1836 345-347 CD denotes 12
T1837 347-348 -RRB- denotes ]
T1838 348-349 . denotes .
T1839 349-471 sentence denotes To assess this molecular phenotype in CbCln3Δex7/8 cells, RT-PCR and anti-battenin (batp1) immunostaining were performed.
T1840 350-352 TO denotes To
T1841 353-359 VB denotes assess
T1843 360-364 DT denotes this
T1845 365-374 JJ denotes molecular
T1844 375-384 NN denotes phenotype
T1846 385-387 IN denotes in
T1847 388-398 NN denotes CbCln3Δex7
T1849 398-399 HYPH denotes /
T1850 399-400 CD denotes 8
T1848 401-406 NNS denotes cells
T1851 406-408 , denotes ,
T1852 408-410 NN denotes RT
T1854 410-411 HYPH denotes -
T1853 411-414 NN denotes PCR
T1855 415-418 CC denotes and
T1856 419-432 JJ denotes anti-battenin
T1858 433-434 -LRB- denotes (
T1859 434-439 NN denotes batp1
T1860 439-440 -RRB- denotes )
T1857 441-455 NN denotes immunostaining
T1861 456-460 VBD denotes were
T1842 461-470 VBN denotes performed
T1862 470-471 . denotes .
T1863 471-678 sentence denotes As shown in Figure 2, Cln3 mRNA isoforms in wild-type and homozygous cells were similar to those observed in total RNA isolated from wild-type or homozygous Cln3Δex7/8 knock-in brain, respectively (Fig. 2).
T1864 472-474 IN denotes As
T1865 475-480 VBN denotes shown
T1867 481-483 IN denotes in
T1868 484-490 NN denotes Figure
T1869 491-492 CD denotes 2
T1870 492-494 , denotes ,
T1871 494-498 NN denotes Cln3
T1873 499-503 NN denotes mRNA
T1872 504-512 NNS denotes isoforms
T1874 513-515 IN denotes in
T1875 516-520 JJ denotes wild
T1877 520-521 HYPH denotes -
T1876 521-525 NN denotes type
T1879 526-529 CC denotes and
T1880 530-540 JJ denotes homozygous
T1878 541-546 NNS denotes cells
T1866 547-551 VBD denotes were
T1881 552-559 JJ denotes similar
T1882 560-562 IN denotes to
T1883 563-568 DT denotes those
T1884 569-577 VBN denotes observed
T1885 578-580 IN denotes in
T1886 581-586 JJ denotes total
T1887 587-590 NN denotes RNA
T1888 591-599 VBN denotes isolated
T1889 600-604 IN denotes from
T1890 605-609 JJ denotes wild
T1892 609-610 HYPH denotes -
T1891 610-614 NN denotes type
T1894 615-617 CC denotes or
T1895 618-628 JJ denotes homozygous
T1896 629-637 NN denotes Cln3Δex7
T1897 637-638 HYPH denotes /
T1898 638-639 CD denotes 8
T1899 640-645 VB denotes knock
T1900 645-646 HYPH denotes -
T1901 646-648 RP denotes in
T1893 649-654 NN denotes brain
T1902 654-656 , denotes ,
T1903 656-668 RB denotes respectively
T1904 669-670 -LRB- denotes (
T1905 670-674 NN denotes Fig.
T1906 675-676 CD denotes 2
T1907 676-677 -RRB- denotes )
T1908 677-678 . denotes .
T1909 678-880 sentence denotes In addition, batp1 immunostaining detected mutant battenin product in homozygous CbCln3Δex7/8 cells, in a similar albeit reduced cytoplasmic, vesicular staining pattern as that seen in wild-type cells.
T1910 679-681 IN denotes In
T1912 682-690 NN denotes addition
T1913 690-692 , denotes ,
T1914 692-697 NN denotes batp1
T1915 698-712 NN denotes immunostaining
T1911 713-721 VBD denotes detected
T1916 722-728 NN denotes mutant
T1918 729-737 NN denotes battenin
T1917 738-745 NN denotes product
T1919 746-748 IN denotes in
T1920 749-759 JJ denotes homozygous
T1922 760-770 NN denotes CbCln3Δex7
T1923 770-771 HYPH denotes /
T1924 771-772 CD denotes 8
T1921 773-778 NNS denotes cells
T1925 778-780 , denotes ,
T1926 780-782 IN denotes in
T1927 783-784 DT denotes a
T1929 785-792 JJ denotes similar
T1930 793-799 IN denotes albeit
T1931 800-807 VBN denotes reduced
T1932 808-819 JJ denotes cytoplasmic
T1933 819-821 , denotes ,
T1934 821-830 JJ denotes vesicular
T1935 831-839 NN denotes staining
T1928 840-847 NN denotes pattern
T1936 848-850 IN denotes as
T1937 851-855 DT denotes that
T1938 856-860 VBN denotes seen
T1939 861-863 IN denotes in
T1940 864-868 JJ denotes wild
T1942 868-869 HYPH denotes -
T1941 869-873 NN denotes type
T1943 874-879 NNS denotes cells
T1944 879-880 . denotes .
T1945 880-1066 sentence denotes Batp1 signal exhibited some overlap with the lysosomal marker, Lamp1, but had more significant overlap with early endosome antigen 1 (EEA1) and the late endosomal marker, Rab7 (Fig. 3).
T1946 881-886 NN denotes Batp1
T1947 887-893 NN denotes signal
T1948 894-903 VBD denotes exhibited
T1949 904-908 DT denotes some
T1950 909-916 NN denotes overlap
T1951 917-921 IN denotes with
T1952 922-925 DT denotes the
T1954 926-935 JJ denotes lysosomal
T1953 936-942 NN denotes marker
T1955 942-944 , denotes ,
T1956 944-949 NN denotes Lamp1
T1957 949-951 , denotes ,
T1958 951-954 CC denotes but
T1959 955-958 VBD denotes had
T1960 959-963 RBR denotes more
T1961 964-975 JJ denotes significant
T1962 976-983 NN denotes overlap
T1963 984-988 IN denotes with
T1964 989-994 JJ denotes early
T1966 995-1003 NN denotes endosome
T1965 1004-1011 NN denotes antigen
T1967 1012-1013 CD denotes 1
T1968 1014-1015 -LRB- denotes (
T1969 1015-1019 NN denotes EEA1
T1970 1019-1020 -RRB- denotes )
T1971 1021-1024 CC denotes and
T1972 1025-1028 DT denotes the
T1974 1029-1033 JJ denotes late
T1975 1034-1043 JJ denotes endosomal
T1973 1044-1050 NN denotes marker
T1976 1050-1052 , denotes ,
T1977 1052-1056 NN denotes Rab7
T1978 1057-1058 -LRB- denotes (
T1979 1058-1062 NN denotes Fig.
T1980 1063-1064 CD denotes 3
T1981 1064-1065 -RRB- denotes )
T1982 1065-1066 . denotes .
T1983 1066-1194 sentence denotes Only limited overlap was observed with recycling endosomes, as determined by transferrin receptor co-staining (data not shown).
T1984 1067-1071 RB denotes Only
T1986 1072-1079 VBN denotes limited
T1985 1080-1087 NN denotes overlap
T1988 1088-1091 VBD denotes was
T1987 1092-1100 VBN denotes observed
T1989 1101-1105 IN denotes with
T1990 1106-1115 VBG denotes recycling
T1991 1116-1125 NNS denotes endosomes
T1992 1125-1127 , denotes ,
T1993 1127-1129 IN denotes as
T1994 1130-1140 VBN denotes determined
T1995 1141-1143 IN denotes by
T1996 1144-1155 NN denotes transferrin
T1997 1156-1164 NN denotes receptor
T1998 1165-1176 NN denotes co-staining
T1999 1177-1178 -LRB- denotes (
T2001 1178-1182 NNS denotes data
T2002 1183-1186 RB denotes not
T2000 1187-1192 VBN denotes shown
T2003 1192-1193 -RRB- denotes )
T2004 1193-1194 . denotes .
T2005 1194-1449 sentence denotes Intriguingly, Lamp1 and EEA1 immunocytochemical distribution were altered in homozygous CbCln3Δex7/8 cells, with less perinuclear clustering than in wild-type cells, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells (Fig. 3).
T2006 1195-1207 RB denotes Intriguingly
T2008 1207-1209 , denotes ,
T2009 1209-1214 NN denotes Lamp1
T2011 1215-1218 CC denotes and
T2012 1219-1223 NN denotes EEA1
T2013 1224-1242 JJ denotes immunocytochemical
T2010 1243-1255 NN denotes distribution
T2014 1256-1260 VBD denotes were
T2007 1261-1268 VBN denotes altered
T2015 1269-1271 IN denotes in
T2016 1272-1282 JJ denotes homozygous
T2018 1283-1293 NN denotes CbCln3Δex7
T2019 1293-1294 HYPH denotes /
T2020 1294-1295 CD denotes 8
T2017 1296-1301 NNS denotes cells
T2021 1301-1303 , denotes ,
T2022 1303-1307 IN denotes with
T2023 1308-1312 JJR denotes less
T2025 1313-1324 JJ denotes perinuclear
T2024 1325-1335 NN denotes clustering
T2026 1336-1340 IN denotes than
T2027 1341-1343 IN denotes in
T2028 1344-1348 JJ denotes wild
T2030 1348-1349 HYPH denotes -
T2029 1349-1353 NN denotes type
T2031 1354-1359 NNS denotes cells
T2032 1359-1361 , denotes ,
T2033 1361-1364 CC denotes and
T2034 1365-1369 NN denotes Rab7
T2035 1370-1378 NN denotes staining
T2036 1379-1382 VBD denotes was
T2037 1383-1393 RB denotes frequently
T2038 1394-1398 RBR denotes less
T2039 1399-1406 JJ denotes intense
T2040 1407-1409 IN denotes in
T2041 1410-1420 JJ denotes homozygous
T2043 1421-1431 NN denotes CbCln3Δex7
T2044 1431-1432 HYPH denotes /
T2045 1432-1433 CD denotes 8
T2042 1434-1439 NNS denotes cells
T2046 1440-1441 -LRB- denotes (
T2047 1441-1445 NN denotes Fig.
T2048 1446-1447 CD denotes 3
T2049 1447-1448 -RRB- denotes )
T2050 1448-1449 . denotes .
T2051 1449-1656 sentence denotes Heterozygous CbCln3Δex7/8 cells contained a mixture of Cln3 mRNA products from both the wild-type allele and the mutant allele, and batp1 signal was similar to that seen in wild-type cells (data not shown).
T2052 1450-1462 JJ denotes Heterozygous
T2054 1463-1473 NN denotes CbCln3Δex7
T2055 1473-1474 HYPH denotes /
T2056 1474-1475 CD denotes 8
T2053 1476-1481 NNS denotes cells
T2057 1482-1491 VBD denotes contained
T2058 1492-1493 DT denotes a
T2059 1494-1501 NN denotes mixture
T2060 1502-1504 IN denotes of
T2061 1505-1509 NN denotes Cln3
T2063 1510-1514 NN denotes mRNA
T2062 1515-1523 NNS denotes products
T2064 1524-1528 IN denotes from
T2065 1529-1533 CC denotes both
T2067 1534-1537 DT denotes the
T2068 1538-1542 JJ denotes wild
T2070 1542-1543 HYPH denotes -
T2069 1543-1547 NN denotes type
T2066 1548-1554 NN denotes allele
T2071 1555-1558 CC denotes and
T2072 1559-1562 DT denotes the
T2074 1563-1569 NN denotes mutant
T2073 1570-1576 NN denotes allele
T2075 1576-1578 , denotes ,
T2076 1578-1581 CC denotes and
T2077 1582-1587 NN denotes batp1
T2078 1588-1594 NN denotes signal
T2079 1595-1598 VBD denotes was
T2080 1599-1606 JJ denotes similar
T2081 1607-1609 IN denotes to
T2082 1610-1614 DT denotes that
T2083 1615-1619 VBN denotes seen
T2084 1620-1622 IN denotes in
T2085 1623-1627 JJ denotes wild
T2087 1627-1628 HYPH denotes -
T2086 1628-1632 NN denotes type
T2088 1633-1638 NNS denotes cells
T2089 1639-1640 -LRB- denotes (
T2091 1640-1644 NNS denotes data
T2092 1645-1648 RB denotes not
T2090 1649-1654 VBN denotes shown
T2093 1654-1655 -RRB- denotes )
T2094 1655-1656 . denotes .
T2095 1656-3449 sentence denotes Figure 2 RT-PCR of Cln3 mRNA in wild-type and homozygous CbCln3Δex7/8 cells Cln3 Exon1-forward, Exon 15-reverse RT-PCR products are shown, from total wild-type (+/+) or homozygous mutant (Δex7/8/Δex7/8) brain and cell line RNA. Brain and cell line RT-PCR reaction products had identical band patterns on ethidium-bromide stained agarose gels. Wild-type RT-PCR product was a single ~1.6 kb band and mutant products were ~1.6, ~1.5, ~1.4, ~1.35, and ~1.3 kb, representing multiple mutant splice variants. Figure 3 Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. During sub-confluent growth conditions, neither wild-type nor homozygous CbCln3Δex7/8 cells displayed autofluorescence or subunit c inclusion formation (data not shown).
T8215 1667-1669 NN denotes RT
T8217 1669-1670 HYPH denotes -
T8216 1670-1673 NN denotes PCR
T8219 1674-1676 IN denotes of
T8220 1677-1681 NN denotes Cln3
T8221 1682-1686 NN denotes mRNA
T8222 1687-1689 IN denotes in
T8223 1690-1694 JJ denotes wild
T8225 1694-1695 HYPH denotes -
T8224 1695-1699 NN denotes type
T8227 1700-1703 CC denotes and
T8228 1704-1714 JJ denotes homozygous
T8229 1715-1725 NN denotes CbCln3Δex7
T8230 1725-1726 HYPH denotes /
T8231 1726-1727 CD denotes 8
T8226 1728-1733 NNS denotes cells
T8232 1734-1738 NN denotes Cln3
T8234 1739-1744 NN denotes Exon1
T8236 1744-1745 HYPH denotes -
T8235 1745-1752 JJ denotes forward
T8238 1752-1754 , denotes ,
T8239 1754-1758 NN denotes Exon
T8240 1759-1761 CD denotes 15
T8241 1761-1762 HYPH denotes -
T8237 1762-1769 JJ denotes reverse
T8242 1770-1772 NN denotes RT
T8244 1772-1773 HYPH denotes -
T8243 1773-1776 NN denotes PCR
T8233 1777-1785 NNS denotes products
T8245 1786-1789 VBP denotes are
T8218 1790-1795 VBN denotes shown
T8246 1795-1797 , denotes ,
T8247 1797-1801 IN denotes from
T8248 1802-1807 JJ denotes total
T8250 1808-1812 JJ denotes wild
T8252 1812-1813 HYPH denotes -
T8251 1813-1817 NN denotes type
T8253 1818-1819 -LRB- denotes (
T8254 1819-1820 SYM denotes +
T8256 1820-1821 HYPH denotes /
T8255 1821-1822 SYM denotes +
T8257 1822-1823 -RRB- denotes )
T8258 1824-1826 CC denotes or
T8259 1827-1837 JJ denotes homozygous
T8260 1838-1844 NN denotes mutant
T8261 1845-1846 -LRB- denotes (
T8262 1846-1850 NN denotes Δex7
T8263 1850-1851 HYPH denotes /
T8264 1851-1852 CD denotes 8
T8265 1852-1853 HYPH denotes /
T8266 1853-1857 NN denotes Δex7
T8267 1857-1858 HYPH denotes /
T8268 1858-1859 CD denotes 8
T8269 1859-1860 -RRB- denotes )
T8270 1861-1866 NN denotes brain
T8271 1867-1870 CC denotes and
T8272 1871-1875 NN denotes cell
T8273 1876-1880 NN denotes line
T8249 1881-1884 NN denotes RNA
T8274 1884-1885 . denotes .
T8275 1885-2000 sentence denotes Brain and cell line RT-PCR reaction products had identical band patterns on ethidium-bromide stained agarose gels.
T8276 1886-1891 NN denotes Brain
T8278 1892-1895 CC denotes and
T8279 1896-1900 NN denotes cell
T8280 1901-1905 NN denotes line
T8281 1906-1908 NN denotes RT
T8283 1908-1909 HYPH denotes -
T8282 1909-1912 NN denotes PCR
T8284 1913-1921 NN denotes reaction
T8277 1922-1930 NNS denotes products
T8285 1931-1934 VBD denotes had
T8286 1935-1944 JJ denotes identical
T8288 1945-1949 NN denotes band
T8287 1950-1958 NNS denotes patterns
T8289 1959-1961 IN denotes on
T8290 1962-1970 NN denotes ethidium
T8292 1970-1971 HYPH denotes -
T8291 1971-1978 NN denotes bromide
T8293 1979-1986 VBN denotes stained
T8295 1987-1994 NN denotes agarose
T8294 1995-1999 NNS denotes gels
T8296 1999-2000 . denotes .
T8297 2000-2160 sentence denotes Wild-type RT-PCR product was a single ~1.6 kb band and mutant products were ~1.6, ~1.5, ~1.4, ~1.35, and ~1.3 kb, representing multiple mutant splice variants.
T8298 2001-2005 JJ denotes Wild
T8300 2005-2006 HYPH denotes -
T8299 2006-2010 NN denotes type
T8302 2011-2013 NN denotes RT
T8304 2013-2014 HYPH denotes -
T8303 2014-2017 NN denotes PCR
T8301 2018-2025 NN denotes product
T8305 2026-2029 VBD denotes was
T8306 2030-2031 DT denotes a
T8308 2032-2038 JJ denotes single
T8309 2039-2040 SYM denotes ~
T8310 2040-2043 CD denotes 1.6
T8311 2044-2046 NN denotes kb
T8307 2047-2051 NN denotes band
T8312 2052-2055 CC denotes and
T8313 2056-2062 NN denotes mutant
T8314 2063-2071 NNS denotes products
T8315 2072-2076 VBD denotes were
T8316 2077-2078 SYM denotes ~
T8317 2078-2081 CD denotes 1.6
T8319 2081-2083 , denotes ,
T8320 2083-2084 SYM denotes ~
T8321 2084-2087 CD denotes 1.5
T8322 2087-2089 , denotes ,
T8323 2089-2090 SYM denotes ~
T8324 2090-2093 CD denotes 1.4
T8325 2093-2095 , denotes ,
T8326 2095-2096 SYM denotes ~
T8327 2096-2100 CD denotes 1.35
T8328 2100-2102 , denotes ,
T8329 2102-2105 CC denotes and
T8330 2106-2107 SYM denotes ~
T8331 2107-2110 NN denotes 1.3
T8318 2111-2113 NN denotes kb
T8332 2113-2115 , denotes ,
T8333 2115-2127 VBG denotes representing
T8334 2128-2136 JJ denotes multiple
T8336 2137-2143 NN denotes mutant
T8337 2144-2150 NN denotes splice
T8335 2151-2159 NNS denotes variants
T8338 2159-2160 . denotes .
T8445 2171-2179 NN denotes Battenin
T8447 2180-2183 CC denotes and
T8448 2184-2193 JJ denotes lysosomal
T8449 2194-2197 CC denotes and
T8450 2198-2207 JJ denotes endosomal
T8446 2208-2214 NN denotes marker
T8451 2215-2226 NN denotes co-staining
T8453 2227-2229 IN denotes in
T8454 2230-2234 JJ denotes wild
T8456 2234-2235 HYPH denotes -
T8455 2235-2239 NN denotes type
T8458 2240-2243 CC denotes and
T8459 2244-2254 JJ denotes homozygous
T8460 2255-2265 NN denotes CbCln3Δex7
T8462 2265-2266 HYPH denotes /
T8463 2266-2267 CD denotes 8
T8464 2268-2278 JJ denotes cerebellar
T8461 2279-2288 NN denotes precursor
T8457 2289-2294 NNS denotes cells
T8465 2295-2300 NN denotes Batp1
T8466 2301-2315 NN denotes immunostaining
T8467 2316-2318 IN denotes of
T8468 2319-2323 JJ denotes wild
T8470 2323-2324 HYPH denotes -
T8469 2324-2328 NN denotes type
T8471 2329-2330 -LRB- denotes (
T8472 2330-2336 NN denotes CbCln3
T8473 2336-2337 SYM denotes +
T8474 2337-2338 HYPH denotes /
T8475 2338-2339 SYM denotes +
T8476 2339-2340 -RRB- denotes )
T8477 2341-2344 CC denotes and
T8478 2345-2355 JJ denotes homozygous
T8479 2356-2362 NN denotes mutant
T8481 2363-2364 -LRB- denotes (
T8482 2364-2374 NN denotes CbCln3Δex7
T8484 2374-2375 HYPH denotes /
T8485 2375-2376 CD denotes 8
T8486 2376-2377 HYPH denotes /
T8483 2377-2381 NN denotes Δex7
T8487 2381-2382 HYPH denotes /
T8488 2382-2383 CD denotes 8
T8489 2383-2384 -RRB- denotes )
T8490 2385-2395 JJ denotes cerebellar
T8491 2396-2405 NN denotes precursor
T8480 2406-2411 NNS denotes cells
T8492 2412-2414 VBZ denotes is
T8452 2415-2420 VBN denotes shown
T8493 2420-2422 , denotes ,
T8494 2422-2426 IN denotes with
T8495 2427-2438 NN denotes co-staining
T8496 2439-2442 IN denotes for
T8497 2443-2452 NNS denotes lysosomes
T8498 2453-2454 -LRB- denotes (
T8499 2454-2458 NN denotes Lamp
T8500 2459-2460 CD denotes 1
T8501 2460-2461 -RRB- denotes )
T8502 2461-2463 , denotes ,
T8503 2463-2468 JJ denotes early
T8504 2469-2478 NNS denotes endosomes
T8505 2479-2480 -LRB- denotes (
T8506 2480-2484 NN denotes EEA1
T8507 2484-2485 -RRB- denotes )
T8508 2485-2487 , denotes ,
T8509 2487-2490 CC denotes and
T8510 2491-2495 JJ denotes late
T8511 2496-2505 NNS denotes endosomes
T8512 2506-2507 -LRB- denotes (
T8513 2507-2511 NN denotes Rab7
T8514 2511-2512 -RRB- denotes )
T8515 2512-2513 . denotes .
T8516 2513-2684 sentence denotes Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge).
T8517 2514-2525 JJ denotes Significant
T8518 2526-2533 NN denotes overlap
T8520 2534-2536 IN denotes of
T8521 2537-2542 NN denotes Batp1
T8522 2543-2549 NN denotes signal
T8523 2550-2551 -LRB- denotes (
T8524 2551-2554 JJ denotes red
T8525 2554-2555 -RRB- denotes )
T8526 2556-2560 IN denotes with
T8527 2561-2565 NN denotes EEA1
T8528 2566-2567 -LRB- denotes (
T8530 2567-2572 JJ denotes green
T8531 2572-2574 , denotes ,
T8532 2574-2580 JJ denotes middle
T8529 2581-2587 NNS denotes panels
T8533 2587-2588 -RRB- denotes )
T8534 2589-2592 CC denotes and
T8535 2593-2597 NN denotes Rab7
T8536 2598-2599 -LRB- denotes (
T8538 2599-2604 JJ denotes green
T8539 2604-2606 , denotes ,
T8540 2606-2612 JJ denotes bottom
T8537 2613-2619 NNS denotes panels
T8541 2619-2620 -RRB- denotes )
T8542 2621-2624 MD denotes can
T8543 2625-2627 VB denotes be
T8519 2628-2632 VBN denotes seen
T8544 2633-2635 IN denotes as
T8545 2636-2642 JJ denotes yellow
T8546 2643-2647 WRB denotes when
T8548 2648-2651 DT denotes the
T8550 2652-2655 CD denotes two
T8549 2656-2664 NNS denotes channels
T8551 2665-2668 VBP denotes are
T8547 2669-2675 VBN denotes merged
T8552 2676-2677 -LRB- denotes (
T8553 2677-2682 NNP denotes Merge
T8554 2682-2683 -RRB- denotes )
T8555 2683-2684 . denotes .
T8556 2684-2735 sentence denotes The degree of Batp1 overlap is greatest with Rab7.
T8557 2685-2688 DT denotes The
T8558 2689-2695 NN denotes degree
T8560 2696-2698 IN denotes of
T8561 2699-2704 NN denotes Batp1
T8562 2705-2712 NN denotes overlap
T8559 2713-2715 VBZ denotes is
T8563 2716-2724 JJS denotes greatest
T8564 2725-2729 IN denotes with
T8565 2730-2734 NN denotes Rab7
T8566 2734-2735 . denotes .
T8567 2735-2820 sentence denotes Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen.
T8568 2736-2740 RB denotes Only
T8570 2741-2748 VBN denotes limited
T8569 2749-2756 NN denotes overlap
T8572 2757-2764 IN denotes between
T8573 2765-2770 NN denotes Batp1
T8574 2771-2772 -LRB- denotes (
T8575 2772-2775 JJ denotes red
T8576 2775-2776 -RRB- denotes )
T8577 2777-2780 CC denotes and
T8578 2781-2785 NN denotes Lamp
T8579 2786-2787 CD denotes 1
T8580 2788-2789 -LRB- denotes (
T8582 2789-2794 JJ denotes green
T8583 2794-2796 , denotes ,
T8584 2796-2799 JJ denotes top
T8581 2800-2806 NNS denotes panels
T8585 2806-2807 -RRB- denotes )
T8586 2808-2811 MD denotes can
T8587 2812-2814 VB denotes be
T8571 2815-2819 VBN denotes seen
T8588 2819-2820 . denotes .
T8589 2820-3023 sentence denotes Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels.
T8590 2821-2826 NN denotes Batp1
T8591 2827-2833 NN denotes signal
T8593 2834-2836 IN denotes in
T8594 2837-2847 JJ denotes homozygous
T8596 2848-2858 NN denotes CbCln3Δex7
T8597 2858-2859 HYPH denotes /
T8598 2859-2860 CD denotes 8
T8595 2861-2866 NNS denotes cells
T8592 2867-2869 VBZ denotes is
T8599 2870-2883 RB denotes significantly
T8600 2884-2891 JJ denotes reduced
T8601 2891-2893 , denotes ,
T8602 2893-2896 CC denotes but
T8603 2897-2908 JJ denotes significant
T8604 2909-2916 NN denotes overlap
T8606 2917-2921 IN denotes with
T8607 2922-2926 NN denotes EEA1
T8608 2927-2930 CC denotes and
T8609 2931-2935 NN denotes Rab7
T8610 2935-2937 , denotes ,
T8611 2937-2940 CC denotes and
T8612 2941-2945 RB denotes very
T8613 2946-2952 JJ denotes little
T8615 2953-2957 NNP denotes Lamp
T8616 2958-2959 CD denotes 1
T8614 2960-2967 NN denotes overlap
T8617 2967-2969 , denotes ,
T8618 2969-2972 MD denotes can
T8619 2973-2975 VB denotes be
T8605 2976-2980 VBN denotes seen
T8620 2981-2983 IN denotes as
T8621 2984-2990 JJ denotes yellow
T8622 2991-2993 IN denotes in
T8623 2994-2997 DT denotes the
T8625 2998-3008 JJ denotes respective
T8626 3009-3015 JJ denotes merged
T8624 3016-3022 NNS denotes panels
T8627 3022-3023 . denotes .
T8628 3023-3157 sentence denotes Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells.
T8629 3024-3031 RB denotes Notably
T8631 3031-3033 , denotes ,
T8632 3033-3037 NN denotes Lamp
T8634 3038-3039 CD denotes 1
T8635 3040-3043 CC denotes and
T8636 3044-3048 NN denotes EEA1
T8633 3049-3061 NN denotes localization
T8630 3062-3068 VBP denotes appear
T8637 3069-3076 JJ denotes altered
T8638 3076-3078 , denotes ,
T8639 3078-3081 CC denotes and
T8640 3082-3086 NN denotes Rab7
T8641 3087-3095 NN denotes staining
T8642 3096-3099 VBD denotes was
T8643 3100-3110 RB denotes frequently
T8644 3111-3115 RBR denotes less
T8645 3116-3123 JJ denotes intense
T8646 3124-3126 IN denotes in
T8647 3127-3137 JJ denotes homozygous
T8649 3138-3148 NN denotes CbCln3Δex7
T8650 3148-3149 HYPH denotes /
T8651 3149-3150 CD denotes 8
T8648 3151-3156 NNS denotes cells
T8652 3156-3157 . denotes .
T8653 3157-3259 sentence denotes Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings.
T8654 3158-3162 JJ denotes Wild
T8656 3162-3163 HYPH denotes -
T8655 3163-3167 NN denotes type
T8658 3168-3171 CC denotes and
T8659 3172-3182 JJ denotes homozygous
T8660 3183-3193 NN denotes CbCln3Δex7
T8662 3193-3194 HYPH denotes /
T8663 3194-3195 CD denotes 8
T8664 3196-3204 JJ denotes confocal
T8661 3205-3211 NNS denotes images
T8665 3212-3216 VBD denotes were
T8657 3217-3225 VBN denotes captured
T8666 3226-3230 IN denotes with
T8667 3231-3240 JJ denotes identical
T8669 3241-3249 NN denotes exposure
T8668 3250-3258 NNS denotes settings
T8670 3258-3259 . denotes .
T8671 3259-3279 sentence denotes 60 × magnification.
T8672 3260-3262 CD denotes 60
T8674 3263-3264 SYM denotes ×
T8673 3265-3278 NN denotes magnification
T8675 3278-3279 . denotes .
T2096 3280-3286 IN denotes During
T2098 3287-3300 JJ denotes sub-confluent
T2100 3301-3307 NN denotes growth
T2099 3308-3318 NNS denotes conditions
T2101 3318-3320 , denotes ,
T2102 3320-3327 CC denotes neither
T2104 3328-3332 JJ denotes wild
T2105 3332-3333 HYPH denotes -
T2103 3333-3337 NN denotes type
T2107 3338-3341 CC denotes nor
T2108 3342-3352 JJ denotes homozygous
T2109 3353-3363 NN denotes CbCln3Δex7
T2110 3363-3364 HYPH denotes /
T2111 3364-3365 CD denotes 8
T2106 3366-3371 NNS denotes cells
T2097 3372-3381 VBD denotes displayed
T2112 3382-3398 NN denotes autofluorescence
T2114 3399-3401 CC denotes or
T2115 3402-3409 NN denotes subunit
T2116 3410-3411 NN denotes c
T2117 3412-3421 NN denotes inclusion
T2113 3422-3431 NN denotes formation
T2118 3432-3433 -LRB- denotes (
T2120 3433-3437 NNS denotes data
T2121 3438-3441 RB denotes not
T2119 3442-3447 VBN denotes shown
T2122 3447-3448 -RRB- denotes )
T2123 3448-3449 . denotes .
T2124 3449-3678 sentence denotes However, when cells were aged at confluency (3+ days post-confluency), homozygous CbCln3Δex7/8 cellular subunit c levels were elevated beyond normal wild-type levels by immunostaining (Fig. 4a) and immunoblot analysis (Fig. 4b).
T2125 3450-3457 RB denotes However
T2127 3457-3459 , denotes ,
T2128 3459-3463 WRB denotes when
T2130 3464-3469 NNS denotes cells
T2131 3470-3474 VBD denotes were
T2129 3475-3479 VBN denotes aged
T2132 3480-3482 IN denotes at
T2133 3483-3493 NN denotes confluency
T2134 3494-3495 -LRB- denotes (
T2135 3495-3496 CD denotes 3
T2137 3496-3497 SYM denotes +
T2136 3498-3502 NNS denotes days
T2138 3503-3518 RB denotes post-confluency
T2139 3518-3519 -RRB- denotes )
T2140 3519-3521 , denotes ,
T2141 3521-3531 JJ denotes homozygous
T2143 3532-3542 NN denotes CbCln3Δex7
T2144 3542-3543 HYPH denotes /
T2145 3543-3544 CD denotes 8
T2146 3545-3553 JJ denotes cellular
T2147 3554-3561 NN denotes subunit
T2148 3562-3563 NN denotes c
T2142 3564-3570 NNS denotes levels
T2149 3571-3575 VBD denotes were
T2126 3576-3584 VBN denotes elevated
T2150 3585-3591 IN denotes beyond
T2151 3592-3598 JJ denotes normal
T2153 3599-3603 JJ denotes wild
T2155 3603-3604 HYPH denotes -
T2154 3604-3608 NN denotes type
T2152 3609-3615 NNS denotes levels
T2156 3616-3618 IN denotes by
T2157 3619-3633 NN denotes immunostaining
T2158 3634-3635 -LRB- denotes (
T2160 3635-3639 NN denotes Fig.
T2159 3640-3642 NN denotes 4a
T2161 3642-3643 -RRB- denotes )
T2162 3644-3647 CC denotes and
T2163 3648-3658 NN denotes immunoblot
T2164 3659-3667 NN denotes analysis
T2165 3668-3669 -LRB- denotes (
T2167 3669-3673 NN denotes Fig.
T2166 3674-3676 NN denotes 4b
T2168 3676-3677 -RRB- denotes )
T2169 3677-3678 . denotes .
T2170 3678-3793 sentence denotes Autofluorescent signal sometimes overlapped with subunit c signal, but also was elevated more diffusely (Fig. 4a).
T2171 3679-3694 JJ denotes Autofluorescent
T2172 3695-3701 NN denotes signal
T2174 3702-3711 RB denotes sometimes
T2173 3712-3722 VBD denotes overlapped
T2175 3723-3727 IN denotes with
T2176 3728-3735 NN denotes subunit
T2177 3736-3737 NN denotes c
T2178 3738-3744 NN denotes signal
T2179 3744-3746 , denotes ,
T2180 3746-3749 CC denotes but
T2181 3750-3754 RB denotes also
T2182 3755-3758 VBD denotes was
T2183 3759-3767 JJ denotes elevated
T2184 3768-3772 RBR denotes more
T2185 3773-3782 RB denotes diffusely
T2186 3783-3784 -LRB- denotes (
T2188 3784-3788 NN denotes Fig.
T2187 3789-3791 NN denotes 4a
T2189 3791-3792 -RRB- denotes )
T2190 3792-3793 . denotes .
T2191 3793-4155 sentence denotes Moreover, although multilamellar "fingerprint" profiles were not detected, confluency-aged homozygous CbCln3Δex7/8 cells displayed numerous ultrastructural abnormalities including electron dense inclusions characteristic of lipofuscin and large autophagosomes that contained dense core structures, degenerating mitochondria, and many smaller vesicles (Fig. 4c).
T2192 3794-3802 RB denotes Moreover
T2194 3802-3804 , denotes ,
T2195 3804-3812 IN denotes although
T2197 3813-3826 JJ denotes multilamellar
T2199 3827-3828 `` denotes "
T2200 3828-3839 NN denotes fingerprint
T2201 3839-3840 '' denotes "
T2198 3841-3849 NNS denotes profiles
T2202 3850-3854 VBD denotes were
T2203 3855-3858 RB denotes not
T2196 3859-3867 VBN denotes detected
T2204 3867-3869 , denotes ,
T2205 3869-3879 JJ denotes confluency
T2207 3879-3880 HYPH denotes -
T2206 3880-3884 VBN denotes aged
T2209 3885-3895 JJ denotes homozygous
T2210 3896-3906 NN denotes CbCln3Δex7
T2211 3906-3907 HYPH denotes /
T2212 3907-3908 CD denotes 8
T2208 3909-3914 NNS denotes cells
T2193 3915-3924 VBD denotes displayed
T2213 3925-3933 JJ denotes numerous
T2215 3934-3949 JJ denotes ultrastructural
T2214 3950-3963 NNS denotes abnormalities
T2216 3964-3973 VBG denotes including
T2217 3974-3982 NN denotes electron
T2218 3983-3988 JJ denotes dense
T2219 3989-3999 NNS denotes inclusions
T2220 4000-4014 JJ denotes characteristic
T2221 4015-4017 IN denotes of
T2222 4018-4028 NN denotes lipofuscin
T2223 4029-4032 CC denotes and
T2224 4033-4038 JJ denotes large
T2225 4039-4053 NNS denotes autophagosomes
T2226 4054-4058 WDT denotes that
T2227 4059-4068 VBD denotes contained
T2228 4069-4074 JJ denotes dense
T2230 4075-4079 NN denotes core
T2229 4080-4090 NNS denotes structures
T2231 4090-4092 , denotes ,
T2232 4092-4104 VBG denotes degenerating
T2233 4105-4117 NNS denotes mitochondria
T2234 4117-4119 , denotes ,
T2235 4119-4122 CC denotes and
T2236 4123-4127 JJ denotes many
T2238 4128-4135 JJR denotes smaller
T2237 4136-4144 NNS denotes vesicles
T2239 4145-4146 -LRB- denotes (
T2241 4146-4150 NNP denotes Fig.
T2240 4151-4153 NN denotes 4c
T2242 4153-4154 -RRB- denotes )
T2243 4154-4155 . denotes .
T2244 4155-4274 sentence denotes Inclusion bodies and autophagosomes were infrequently observed in confluency-aged wild-type cultures (data not shown).
T2245 4156-4165 NN denotes Inclusion
T2246 4166-4172 NNS denotes bodies
T2248 4173-4176 CC denotes and
T2249 4177-4191 NNS denotes autophagosomes
T2250 4192-4196 VBD denotes were
T2251 4197-4209 RB denotes infrequently
T2247 4210-4218 VBN denotes observed
T2252 4219-4221 IN denotes in
T2253 4222-4232 NN denotes confluency
T2255 4232-4233 HYPH denotes -
T2254 4233-4237 VBN denotes aged
T2257 4238-4242 JJ denotes wild
T2259 4242-4243 HYPH denotes -
T2258 4243-4247 NN denotes type
T2256 4248-4256 NNS denotes cultures
T2260 4257-4258 -LRB- denotes (
T2262 4258-4262 NNS denotes data
T2263 4263-4266 RB denotes not
T2261 4267-4272 VBN denotes shown
T2264 4272-4273 -RRB- denotes )
T2265 4273-4274 . denotes .
T8805 4285-4292 NN denotes Subunit
T8806 4293-4294 NN denotes c
T8807 4295-4307 NN denotes accumulation
T8808 4308-4310 IN denotes in
T8809 4311-4321 JJ denotes homozygous
T8811 4322-4332 NN denotes CbCln3Δex7
T8812 4332-4333 HYPH denotes /
T8813 4333-4334 CD denotes 8
T8814 4335-4345 JJ denotes cerebellar
T8815 4346-4355 NN denotes precursor
T8810 4356-4361 NNS denotes cells
T8816 4361-4489 sentence denotes a. Subunit c immunostaining and autofluorescence of 7-day confluency-aged wild-type and homozygous CbCln3Δex7/8 cells is shown.
T8817 4362-4363 LS denotes a
T8819 4363-4364 . denotes .
T8820 4365-4372 NN denotes Subunit
T8821 4373-4374 NN denotes c
T8822 4375-4389 NN denotes immunostaining
T8823 4390-4393 CC denotes and
T8824 4394-4410 NN denotes autofluorescence
T8825 4411-4413 IN denotes of
T8826 4414-4415 CD denotes 7
T8828 4415-4416 HYPH denotes -
T8827 4416-4419 NN denotes day
T8829 4420-4430 NN denotes confluency
T8831 4430-4431 HYPH denotes -
T8830 4431-4435 VBN denotes aged
T8833 4436-4440 JJ denotes wild
T8835 4440-4441 HYPH denotes -
T8834 4441-4445 NN denotes type
T8836 4446-4449 CC denotes and
T8837 4450-4460 JJ denotes homozygous
T8838 4461-4471 NN denotes CbCln3Δex7
T8839 4471-4472 HYPH denotes /
T8840 4472-4473 CD denotes 8
T8832 4474-4479 NNS denotes cells
T8841 4480-4482 VBZ denotes is
T8818 4483-4488 VBN denotes shown
T8842 4488-4489 . denotes .
T8843 4489-4585 sentence denotes Wild-type cultures (CbCln3+/+) exhibited limited subunit c immunostaining and autofluorescence.
T8844 4490-4494 JJ denotes Wild
T8846 4494-4495 HYPH denotes -
T8845 4495-4499 NN denotes type
T8847 4500-4508 NNS denotes cultures
T8849 4509-4510 -LRB- denotes (
T8850 4510-4516 NN denotes CbCln3
T8851 4516-4517 SYM denotes +
T8852 4517-4518 HYPH denotes /
T8853 4518-4519 SYM denotes +
T8854 4519-4520 -RRB- denotes )
T8848 4521-4530 VBD denotes exhibited
T8855 4531-4538 JJ denotes limited
T8857 4539-4546 NN denotes subunit
T8856 4547-4548 NN denotes c
T8858 4549-4563 NN denotes immunostaining
T8859 4564-4567 CC denotes and
T8860 4568-4584 NN denotes autofluorescence
T8861 4584-4585 . denotes .
T8862 4585-4657 sentence denotes However, CbCln3Δex7/8/Δex7/8 cells contained numerous subunit c puncta.
T8863 4586-4593 RB denotes However
T8865 4593-4595 , denotes ,
T8866 4595-4605 NN denotes CbCln3Δex7
T8868 4605-4606 HYPH denotes /
T8869 4606-4607 CD denotes 8
T8870 4607-4608 HYPH denotes /
T8867 4608-4612 NN denotes Δex7
T8872 4612-4613 HYPH denotes /
T8873 4613-4614 CD denotes 8
T8871 4615-4620 NNS denotes cells
T8864 4621-4630 VBD denotes contained
T8874 4631-4639 JJ denotes numerous
T8876 4640-4647 NN denotes subunit
T8877 4648-4649 NN denotes c
T8875 4650-4656 NNS denotes puncta
T8878 4656-4657 . denotes .
T8879 4657-4804 sentence denotes Autofluorescence (7 days AF) was also significantly elevated (right panels), although limited overlap with subunit c puncta was observed (arrows).
T8880 4658-4674 NN denotes Autofluorescence
T8882 4675-4676 -LRB- denotes (
T8884 4676-4677 CD denotes 7
T8885 4678-4682 NNS denotes days
T8883 4683-4685 NN denotes AF
T8886 4685-4686 -RRB- denotes )
T8881 4687-4690 VBD denotes was
T8887 4691-4695 RB denotes also
T8888 4696-4709 RB denotes significantly
T8889 4710-4718 VBN denotes elevated
T8890 4719-4720 -LRB- denotes (
T8892 4720-4725 JJ denotes right
T8891 4726-4732 NNS denotes panels
T8893 4732-4733 -RRB- denotes )
T8894 4733-4735 , denotes ,
T8895 4735-4743 IN denotes although
T8897 4744-4751 JJ denotes limited
T8898 4752-4759 NN denotes overlap
T8899 4760-4764 IN denotes with
T8900 4765-4772 NN denotes subunit
T8901 4773-4774 NN denotes c
T8902 4775-4781 NNS denotes puncta
T8903 4782-4785 VBD denotes was
T8896 4786-4794 VBN denotes observed
T8904 4795-4796 -LRB- denotes (
T8905 4796-4802 NNS denotes arrows
T8906 4802-4803 -RRB- denotes )
T8907 4803-4804 . denotes .
T8908 4804-4824 sentence denotes 40 × magnification.
T8909 4805-4807 CD denotes 40
T8911 4808-4809 SYM denotes ×
T8910 4810-4823 NN denotes magnification
T8912 4823-4824 . denotes .
T8913 4824-4927 sentence denotes b. Immunoblot analysis of subunit c protein at sub-confluency or 7-day confluency incubation is shown.
T8914 4825-4826 LS denotes b
T8916 4826-4827 . denotes .
T8917 4828-4838 NN denotes Immunoblot
T8918 4839-4847 NN denotes analysis
T8919 4848-4850 IN denotes of
T8920 4851-4858 NN denotes subunit
T8921 4859-4860 NN denotes c
T8922 4861-4868 NN denotes protein
T8923 4869-4871 IN denotes at
T8924 4872-4886 NN denotes sub-confluency
T8926 4887-4889 CC denotes or
T8927 4890-4891 CD denotes 7
T8929 4891-4892 HYPH denotes -
T8928 4892-4895 NN denotes day
T8930 4896-4906 NN denotes confluency
T8925 4907-4917 NN denotes incubation
T8931 4918-4920 VBZ denotes is
T8915 4921-4926 VBN denotes shown
T8932 4926-4927 . denotes .
T8933 4927-5102 sentence denotes Total protein extracts from sub-confluency wild-type (+/+) and homozygous mutant (Δex7/8/Δex7/8) cultures contained approximately equal levels of subunit c protein (α-sub c).
T8934 4928-4933 JJ denotes Total
T8936 4934-4941 NN denotes protein
T8935 4942-4950 NNS denotes extracts
T8938 4951-4955 IN denotes from
T8939 4956-4970 NN denotes sub-confluency
T8941 4971-4975 JJ denotes wild
T8943 4975-4976 HYPH denotes -
T8942 4976-4980 NN denotes type
T8944 4981-4982 -LRB- denotes (
T8945 4982-4983 SYM denotes +
T8947 4983-4984 HYPH denotes /
T8946 4984-4985 SYM denotes +
T8948 4985-4986 -RRB- denotes )
T8949 4987-4990 CC denotes and
T8950 4991-5001 JJ denotes homozygous
T8951 5002-5008 NN denotes mutant
T8952 5009-5010 -LRB- denotes (
T8953 5010-5014 NN denotes Δex7
T8954 5014-5015 HYPH denotes /
T8955 5015-5016 CD denotes 8
T8956 5016-5017 HYPH denotes /
T8957 5017-5021 NN denotes Δex7
T8958 5021-5022 HYPH denotes /
T8959 5022-5023 CD denotes 8
T8960 5023-5024 -RRB- denotes )
T8940 5025-5033 NNS denotes cultures
T8937 5034-5043 VBD denotes contained
T8961 5044-5057 RB denotes approximately
T8962 5058-5063 JJ denotes equal
T8963 5064-5070 NNS denotes levels
T8964 5071-5073 IN denotes of
T8965 5074-5081 NN denotes subunit
T8966 5082-5083 NN denotes c
T8967 5084-5091 NN denotes protein
T8968 5092-5093 -LRB- denotes (
T8969 5093-5094 NN denotes α
T8971 5094-5095 HYPH denotes -
T8972 5095-5098 JJ denotes sub
T8970 5099-5100 NN denotes c
T8973 5100-5101 -RRB- denotes )
T8974 5101-5102 . denotes .
T8975 5102-5265 sentence denotes 7-day confluency extract from homozygous CbCln3Δex7/8 cells (Δex7/8/Δex7/8) had elevated levels of subunit c protein (~1.5X), relative to wild-type extract (+/+).
T8976 5103-5104 CD denotes 7
T8978 5104-5105 HYPH denotes -
T8977 5105-5108 NN denotes day
T8979 5109-5119 NN denotes confluency
T8980 5120-5127 NN denotes extract
T8982 5128-5132 IN denotes from
T8983 5133-5143 JJ denotes homozygous
T8985 5144-5154 NN denotes CbCln3Δex7
T8986 5154-5155 HYPH denotes /
T8987 5155-5156 CD denotes 8
T8984 5157-5162 NNS denotes cells
T8988 5163-5164 -LRB- denotes (
T8989 5164-5168 NN denotes Δex7
T8990 5168-5169 HYPH denotes /
T8991 5169-5170 CD denotes 8
T8992 5170-5171 HYPH denotes /
T8993 5171-5175 NN denotes Δex7
T8994 5175-5176 HYPH denotes /
T8995 5176-5177 CD denotes 8
T8996 5177-5178 -RRB- denotes )
T8981 5179-5182 VBD denotes had
T8997 5183-5191 VBN denotes elevated
T8998 5192-5198 NNS denotes levels
T8999 5199-5201 IN denotes of
T9000 5202-5209 NN denotes subunit
T9001 5210-5211 NN denotes c
T9002 5212-5219 NN denotes protein
T9003 5220-5221 -LRB- denotes (
T9004 5221-5222 SYM denotes ~
T9005 5222-5225 CD denotes 1.5
T9006 5225-5226 SYM denotes X
T9007 5226-5227 -RRB- denotes )
T9008 5227-5229 , denotes ,
T9009 5229-5237 JJ denotes relative
T9010 5238-5240 IN denotes to
T9011 5241-5245 JJ denotes wild
T9013 5245-5246 HYPH denotes -
T9012 5246-5250 NN denotes type
T9014 5251-5258 NN denotes extract
T9015 5259-5260 -LRB- denotes (
T9016 5260-5261 SYM denotes +
T9018 5261-5262 HYPH denotes /
T9017 5262-5263 SYM denotes +
T9019 5263-5264 -RRB- denotes )
T9020 5264-5265 . denotes .
T9021 5265-5341 sentence denotes Protein levels were normalized to cytochrome c oxidase subunit IV (α-cox4).
T9022 5266-5273 NN denotes Protein
T9023 5274-5280 NNS denotes levels
T9025 5281-5285 VBD denotes were
T9024 5286-5296 VBN denotes normalized
T9026 5297-5299 IN denotes to
T9027 5300-5310 NN denotes cytochrome
T9028 5311-5312 NN denotes c
T9029 5313-5320 NN denotes oxidase
T9030 5321-5328 NN denotes subunit
T9031 5329-5331 CD denotes IV
T9032 5332-5333 -LRB- denotes (
T9033 5333-5334 NN denotes α
T9035 5334-5335 HYPH denotes -
T9034 5335-5339 NN denotes cox4
T9036 5339-5340 -RRB- denotes )
T9037 5340-5341 . denotes .
T9038 5341-5436 sentence denotes c. TEM analysis of inclusions in 7-day confluency-aged homozygous CbCln3Δex7/8 cells is shown.
T9039 5342-5343 LS denotes c
T9041 5343-5344 . denotes .
T9042 5345-5348 NN denotes TEM
T9043 5349-5357 NN denotes analysis
T9044 5358-5360 IN denotes of
T9045 5361-5371 NNS denotes inclusions
T9046 5372-5374 IN denotes in
T9047 5375-5376 CD denotes 7
T9049 5376-5377 HYPH denotes -
T9048 5377-5380 NN denotes day
T9050 5381-5391 NN denotes confluency
T9052 5391-5392 HYPH denotes -
T9051 5392-5396 VBN denotes aged
T9054 5397-5407 JJ denotes homozygous
T9055 5408-5418 NN denotes CbCln3Δex7
T9056 5418-5419 HYPH denotes /
T9057 5419-5420 CD denotes 8
T9053 5421-5426 NNS denotes cells
T9058 5427-5429 VBZ denotes is
T9040 5430-5435 VBN denotes shown
T9059 5435-5436 . denotes .
T9060 5436-5626 sentence denotes A large autophagosome contained by double membrane (arrows) is filled with degenerating mitochondria (Md), electron dense cores (left and right of *) and other smaller vesicular structures.
T9061 5437-5438 DT denotes A
T9063 5439-5444 JJ denotes large
T9062 5445-5458 NN denotes autophagosome
T9065 5459-5468 VBN denotes contained
T9066 5469-5471 IN denotes by
T9067 5472-5478 JJ denotes double
T9068 5479-5487 NN denotes membrane
T9069 5488-5489 -LRB- denotes (
T9070 5489-5495 NNS denotes arrows
T9071 5495-5496 -RRB- denotes )
T9072 5497-5499 VBZ denotes is
T9064 5500-5506 VBN denotes filled
T9073 5507-5511 IN denotes with
T9074 5512-5524 VBG denotes degenerating
T9075 5525-5537 NNS denotes mitochondria
T9076 5538-5539 -LRB- denotes (
T9077 5539-5541 NN denotes Md
T9078 5541-5542 -RRB- denotes )
T9079 5542-5544 , denotes ,
T9080 5544-5552 NN denotes electron
T9082 5553-5558 JJ denotes dense
T9081 5559-5564 NNS denotes cores
T9083 5565-5566 -LRB- denotes (
T9084 5566-5570 JJ denotes left
T9085 5571-5574 CC denotes and
T9086 5575-5580 JJ denotes right
T9087 5581-5583 IN denotes of
T9088 5584-5585 SYM denotes *
T9089 5585-5586 -RRB- denotes )
T9090 5587-5590 CC denotes and
T9091 5591-5596 JJ denotes other
T9093 5597-5604 JJR denotes smaller
T9094 5605-5614 JJ denotes vesicular
T9092 5615-5625 NNS denotes structures
T9095 5625-5626 . denotes .
T9096 5626-5712 sentence denotes A large electron-dense inclusion, with a lipofuscin (Ln) appearance, is also present.
T9097 5627-5628 DT denotes A
T9099 5629-5634 JJ denotes large
T9100 5635-5643 NN denotes electron
T9102 5643-5644 HYPH denotes -
T9101 5644-5649 JJ denotes dense
T9098 5650-5659 NN denotes inclusion
T9104 5659-5661 , denotes ,
T9105 5661-5665 IN denotes with
T9106 5666-5667 DT denotes a
T9108 5668-5678 NN denotes lipofuscin
T9109 5679-5680 -LRB- denotes (
T9110 5680-5682 NN denotes Ln
T9111 5682-5683 -RRB- denotes )
T9107 5684-5694 NN denotes appearance
T9112 5694-5696 , denotes ,
T9103 5696-5698 VBZ denotes is
T9113 5699-5703 RB denotes also
T9114 5704-5711 JJ denotes present
T9115 5711-5712 . denotes .
T9116 5712-5729 sentence denotes M, mitochondria.
T9117 5713-5714 NN denotes M
T9118 5714-5716 , denotes ,
T9119 5716-5728 NNS denotes mitochondria
T9120 5728-5729 . denotes .
T9121 5729-5753 sentence denotes 10,000 × magnification.
T9122 5730-5736 CD denotes 10,000
T9124 5737-5738 SYM denotes ×
T9123 5739-5752 NN denotes magnification
T9125 5752-5753 . denotes .
R1000 T1871 T1872 compound Cln3,isoforms
R1001 T1872 T1866 nsubj isoforms,were
R1002 T1873 T1872 compound mRNA,isoforms
R1003 T1874 T1872 prep in,isoforms
R1004 T1875 T1876 amod wild,type
R1005 T1876 T1878 nmod type,cells
R1006 T1877 T1876 punct -,type
R1007 T1878 T1874 pobj cells,in
R1008 T1879 T1876 cc and,type
R1009 T1880 T1876 conj homozygous,type
R1010 T1881 T1866 acomp similar,were
R1011 T1882 T1881 prep to,similar
R1012 T1883 T1882 pobj those,to
R1013 T1884 T1883 acl observed,those
R1014 T1885 T1884 prep in,observed
R1015 T1886 T1887 amod total,RNA
R1016 T1887 T1885 pobj RNA,in
R1017 T1888 T1887 acl isolated,RNA
R1018 T1889 T1888 prep from,isolated
R1019 T1890 T1891 amod wild,type
R1020 T1891 T1893 nmod type,brain
R1021 T1892 T1891 punct -,type
R1022 T1893 T1889 pobj brain,from
R1023 T1894 T1891 cc or,type
R1024 T1895 T1891 conj homozygous,type
R1025 T1896 T1893 nmod Cln3Δex7,brain
R1026 T1897 T1896 punct /,Cln3Δex7
R1027 T1898 T1896 nummod 8,Cln3Δex7
R1028 T1899 T1893 amod knock,brain
R1029 T1900 T1899 punct -,knock
R1030 T1901 T1899 prt in,knock
R1031 T1902 T1866 punct ", ",were
R1032 T1903 T1866 advmod respectively,were
R1033 T1904 T1905 punct (,Fig.
R1034 T1905 T1866 parataxis Fig.,were
R1035 T1906 T1905 nummod 2,Fig.
R1036 T1907 T1905 punct ),Fig.
R1037 T1908 T1866 punct .,were
R1038 T1910 T1911 prep In,detected
R1039 T1912 T1910 pobj addition,In
R1040 T1913 T1911 punct ", ",detected
R1041 T1914 T1915 compound batp1,immunostaining
R1042 T1915 T1911 nsubj immunostaining,detected
R1043 T1916 T1917 compound mutant,product
R1044 T1917 T1911 dobj product,detected
R1045 T1918 T1917 compound battenin,product
R1046 T1919 T1917 prep in,product
R1047 T1920 T1921 amod homozygous,cells
R1048 T1921 T1919 pobj cells,in
R1049 T1922 T1921 nmod CbCln3Δex7,cells
R1050 T1923 T1922 punct /,CbCln3Δex7
R1051 T1924 T1922 nummod 8,CbCln3Δex7
R1052 T1925 T1917 punct ", ",product
R1053 T1926 T1917 prep in,product
R1054 T1927 T1928 det a,pattern
R1055 T1928 T1926 pobj pattern,in
R1056 T1929 T1928 amod similar,pattern
R1057 T1930 T1931 mark albeit,reduced
R1058 T1931 T1929 parataxis reduced,similar
R1059 T1932 T1928 amod cytoplasmic,pattern
R1060 T1933 T1928 punct ", ",pattern
R1061 T1934 T1928 amod vesicular,pattern
R1062 T1935 T1928 compound staining,pattern
R1063 T1936 T1928 prep as,pattern
R1064 T1937 T1936 pobj that,as
R1065 T1938 T1937 acl seen,that
R1066 T1939 T1938 prep in,seen
R1067 T1940 T1941 amod wild,type
R1068 T1941 T1943 compound type,cells
R1069 T1942 T1941 punct -,type
R1070 T1943 T1939 pobj cells,in
R1071 T1944 T1911 punct .,detected
R1072 T1946 T1947 compound Batp1,signal
R1073 T1947 T1948 nsubj signal,exhibited
R1074 T1949 T1950 det some,overlap
R1075 T1950 T1948 dobj overlap,exhibited
R1076 T1951 T1948 prep with,exhibited
R1077 T1952 T1953 det the,marker
R1078 T1953 T1951 pobj marker,with
R1079 T1954 T1953 amod lysosomal,marker
R1080 T1955 T1953 punct ", ",marker
R1081 T1956 T1953 appos Lamp1,marker
R1082 T1957 T1948 punct ", ",exhibited
R1083 T1958 T1948 cc but,exhibited
R1084 T1959 T1948 conj had,exhibited
R1085 T1960 T1961 advmod more,significant
R1086 T1961 T1962 amod significant,overlap
R1087 T1962 T1959 dobj overlap,had
R1088 T1963 T1959 prep with,had
R1089 T1964 T1965 amod early,antigen
R1090 T1965 T1963 pobj antigen,with
R1091 T1966 T1965 compound endosome,antigen
R1092 T1967 T1965 nummod 1,antigen
R1093 T1968 T1965 punct (,antigen
R1094 T1969 T1965 appos EEA1,antigen
R1095 T1970 T1965 punct ),antigen
R1096 T1971 T1965 cc and,antigen
R1097 T1972 T1973 det the,marker
R1098 T1973 T1965 conj marker,antigen
R1099 T1974 T1973 amod late,marker
R1100 T1975 T1973 amod endosomal,marker
R1101 T1976 T1973 punct ", ",marker
R1102 T1977 T1973 appos Rab7,marker
R1103 T1978 T1979 punct (,Fig.
R1104 T1979 T1959 parataxis Fig.,had
R1105 T1980 T1979 nummod 3,Fig.
R1106 T1981 T1979 punct ),Fig.
R1107 T1982 T1948 punct .,exhibited
R1108 T1984 T1985 advmod Only,overlap
R1109 T1985 T1987 nsubjpass overlap,observed
R1110 T1986 T1985 amod limited,overlap
R1111 T1988 T1987 auxpass was,observed
R1112 T1989 T1987 prep with,observed
R1113 T1990 T1991 amod recycling,endosomes
R1114 T1991 T1989 pobj endosomes,with
R1115 T1992 T1987 punct ", ",observed
R1116 T1993 T1994 mark as,determined
R1117 T1994 T1987 advcl determined,observed
R1118 T1995 T1994 agent by,determined
R1119 T1996 T1997 compound transferrin,receptor
R1120 T1997 T1998 compound receptor,co-staining
R1121 T1998 T1995 pobj co-staining,by
R1122 T1999 T2000 punct (,shown
R1123 T2000 T1987 parataxis shown,observed
R1124 T2001 T2000 nsubj data,shown
R1125 T2002 T2000 neg not,shown
R1126 T2003 T2000 punct ),shown
R1127 T2004 T1987 punct .,observed
R1128 T2006 T2007 advmod Intriguingly,altered
R1129 T2008 T2007 punct ", ",altered
R1130 T2009 T2010 nmod Lamp1,distribution
R1131 T2010 T2007 nsubjpass distribution,altered
R1132 T2011 T2009 cc and,Lamp1
R1133 T2012 T2009 conj EEA1,Lamp1
R1134 T2013 T2010 amod immunocytochemical,distribution
R1135 T2014 T2007 auxpass were,altered
R1136 T2015 T2007 prep in,altered
R1137 T2016 T2017 amod homozygous,cells
R1138 T2017 T2015 pobj cells,in
R1139 T2018 T2017 nmod CbCln3Δex7,cells
R1140 T2019 T2018 punct /,CbCln3Δex7
R1141 T2020 T2018 nummod 8,CbCln3Δex7
R1142 T2021 T2007 punct ", ",altered
R1143 T2022 T2007 prep with,altered
R1144 T2023 T2024 amod less,clustering
R1145 T2024 T2022 pobj clustering,with
R1146 T2025 T2024 amod perinuclear,clustering
R1147 T2026 T2024 prep than,clustering
R1148 T2027 T2026 prep in,than
R1149 T2028 T2029 amod wild,type
R1150 T2029 T2031 compound type,cells
R1151 T2030 T2029 punct -,type
R1152 T2031 T2027 pobj cells,in
R1153 T2032 T2007 punct ", ",altered
R1154 T2033 T2007 cc and,altered
R1155 T2034 T2035 compound Rab7,staining
R1156 T2035 T2036 nsubj staining,was
R1157 T2036 T2007 conj was,altered
R1158 T2037 T2036 advmod frequently,was
R1159 T2038 T2039 advmod less,intense
R1160 T2039 T2036 acomp intense,was
R1161 T2040 T2039 prep in,intense
R1162 T2041 T2042 amod homozygous,cells
R1163 T2042 T2040 pobj cells,in
R1164 T2043 T2042 nmod CbCln3Δex7,cells
R1165 T2044 T2043 punct /,CbCln3Δex7
R1166 T2045 T2043 nummod 8,CbCln3Δex7
R1167 T2046 T2047 punct (,Fig.
R1168 T2047 T2036 parataxis Fig.,was
R1169 T2048 T2047 nummod 3,Fig.
R1170 T2049 T2047 punct ),Fig.
R1171 T2050 T2036 punct .,was
R1172 T2052 T2053 amod Heterozygous,cells
R1173 T2053 T2057 nsubj cells,contained
R1174 T2054 T2053 nmod CbCln3Δex7,cells
R1175 T2055 T2054 punct /,CbCln3Δex7
R1176 T2056 T2054 nummod 8,CbCln3Δex7
R1177 T2058 T2059 det a,mixture
R1178 T2059 T2057 dobj mixture,contained
R1179 T2060 T2059 prep of,mixture
R1180 T2061 T2062 compound Cln3,products
R1181 T2062 T2060 pobj products,of
R1182 T2063 T2062 compound mRNA,products
R1183 T2064 T2059 prep from,mixture
R1184 T2065 T2066 preconj both,allele
R1185 T2066 T2064 pobj allele,from
R1186 T2067 T2066 det the,allele
R1187 T2068 T2069 amod wild,type
R1188 T2069 T2066 compound type,allele
R1189 T2070 T2069 punct -,type
R1190 T2071 T2066 cc and,allele
R1191 T2072 T2073 det the,allele
R1192 T2073 T2066 conj allele,allele
R1193 T2074 T2073 compound mutant,allele
R1194 T2075 T2057 punct ", ",contained
R1195 T2076 T2057 cc and,contained
R1196 T2077 T2078 compound batp1,signal
R1197 T2078 T2079 nsubj signal,was
R1198 T2079 T2057 conj was,contained
R1199 T2080 T2079 acomp similar,was
R1200 T2081 T2080 prep to,similar
R1201 T2082 T2081 pobj that,to
R1202 T2083 T2082 acl seen,that
R1203 T2084 T2083 prep in,seen
R1204 T2085 T2086 amod wild,type
R1205 T2086 T2088 compound type,cells
R1206 T2087 T2086 punct -,type
R1207 T2088 T2084 pobj cells,in
R1208 T2089 T2090 punct (,shown
R1209 T2090 T2079 parataxis shown,was
R1210 T2091 T2090 nsubj data,shown
R1211 T2092 T2090 neg not,shown
R1212 T2093 T2090 punct ),shown
R1213 T2094 T2079 punct .,was
R1214 T2096 T2097 prep During,displayed
R1215 T2098 T2099 amod sub-confluent,conditions
R1216 T2099 T2096 pobj conditions,During
R1217 T2100 T2099 compound growth,conditions
R1218 T2101 T2097 punct ", ",displayed
R1219 T2102 T2103 preconj neither,type
R1220 T2103 T2106 nmod type,cells
R1221 T2104 T2103 amod wild,type
R1222 T2105 T2103 punct -,type
R1223 T2106 T2097 nsubj cells,displayed
R1224 T2107 T2103 cc nor,type
R1225 T2108 T2109 amod homozygous,CbCln3Δex7
R1226 T2109 T2103 conj CbCln3Δex7,type
R1227 T2110 T2109 punct /,CbCln3Δex7
R1228 T2111 T2109 nummod 8,CbCln3Δex7
R1229 T2112 T2113 nmod autofluorescence,formation
R1230 T2113 T2097 dobj formation,displayed
R1231 T2114 T2112 cc or,autofluorescence
R1232 T2115 T2116 compound subunit,c
R1233 T2116 T2117 compound c,inclusion
R1234 T2117 T2112 conj inclusion,autofluorescence
R1235 T2118 T2119 punct (,shown
R1236 T2119 T2097 parataxis shown,displayed
R1237 T2120 T2119 nsubj data,shown
R1238 T2121 T2119 neg not,shown
R1239 T2122 T2119 punct ),shown
R1240 T2123 T2097 punct .,displayed
R1241 T2125 T2126 advmod However,elevated
R1242 T2127 T2126 punct ", ",elevated
R1243 T2128 T2129 advmod when,aged
R1244 T2129 T2126 advcl aged,elevated
R1245 T2130 T2129 nsubjpass cells,aged
R1246 T2131 T2129 auxpass were,aged
R1247 T2132 T2129 prep at,aged
R1248 T2133 T2132 pobj confluency,at
R1249 T2134 T2133 punct (,confluency
R1250 T2135 T2136 nummod 3,days
R1251 T2136 T2138 npadvmod days,post-confluency
R1252 T2137 T2135 punct +,3
R1253 T2138 T2133 advmod post-confluency,confluency
R1254 T2139 T2126 punct ),elevated
R1255 T2140 T2126 punct ", ",elevated
R1256 T2141 T2142 amod homozygous,levels
R1257 T2142 T2126 nsubjpass levels,elevated
R1258 T2143 T2142 nmod CbCln3Δex7,levels
R1259 T2144 T2143 punct /,CbCln3Δex7
R1260 T2145 T2143 nummod 8,CbCln3Δex7
R1261 T2146 T2142 amod cellular,levels
R1262 T2147 T2148 compound subunit,c
R1263 T2148 T2142 compound c,levels
R1264 T2149 T2126 auxpass were,elevated
R1265 T2150 T2126 prep beyond,elevated
R1266 T2151 T2152 amod normal,levels
R1267 T2152 T2150 pobj levels,beyond
R1268 T2153 T2154 amod wild,type
R1269 T2154 T2152 compound type,levels
R1270 T2155 T2154 punct -,type
R1271 T2156 T2126 prep by,elevated
R1272 T2157 T2156 pobj immunostaining,by
R1273 T2158 T2159 punct (,4a
R1274 T2159 T2157 parataxis 4a,immunostaining
R1275 T2160 T2159 compound Fig.,4a
R1276 T2161 T2159 punct ),4a
R1277 T2162 T2157 cc and,immunostaining
R1278 T2163 T2164 compound immunoblot,analysis
R1279 T2164 T2157 conj analysis,immunostaining
R1280 T2165 T2166 punct (,4b
R1281 T2166 T2164 parataxis 4b,analysis
R1282 T2167 T2166 compound Fig.,4b
R1283 T2168 T2166 punct ),4b
R1284 T2169 T2126 punct .,elevated
R1285 T2171 T2172 amod Autofluorescent,signal
R1286 T2172 T2173 nsubj signal,overlapped
R1287 T2174 T2173 advmod sometimes,overlapped
R1288 T2175 T2173 prep with,overlapped
R1289 T2176 T2177 compound subunit,c
R1290 T2177 T2178 compound c,signal
R1291 T2178 T2175 pobj signal,with
R1292 T2179 T2173 punct ", ",overlapped
R1293 T2180 T2173 cc but,overlapped
R1294 T2181 T2180 advmod also,but
R1295 T2182 T2173 conj was,overlapped
R1296 T2183 T2182 acomp elevated,was
R1297 T2184 T2185 advmod more,diffusely
R1298 T2185 T2182 advmod diffusely,was
R1299 T2186 T2187 punct (,4a
R1300 T2187 T2182 parataxis 4a,was
R1301 T2188 T2187 compound Fig.,4a
R1302 T2189 T2187 punct ),4a
R1303 T2190 T2173 punct .,overlapped
R1304 T2192 T2193 advmod Moreover,displayed
R1305 T2194 T2193 punct ", ",displayed
R1306 T2195 T2196 mark although,detected
R1307 T2196 T2193 advcl detected,displayed
R1308 T2197 T2198 amod multilamellar,profiles
R1309 T2198 T2196 nsubjpass profiles,detected
R1310 T2199 T2198 punct """",profiles
R1311 T2200 T2198 nmod fingerprint,profiles
R1312 T2201 T2198 punct """",profiles
R1313 T2202 T2196 auxpass were,detected
R1314 T2203 T2196 neg not,detected
R1315 T2204 T2193 punct ", ",displayed
R1316 T2205 T2206 amod confluency,aged
R1317 T2206 T2208 amod aged,cells
R1318 T2207 T2206 punct -,aged
R1319 T2208 T2193 nsubj cells,displayed
R1320 T2209 T2208 amod homozygous,cells
R1321 T2210 T2208 nmod CbCln3Δex7,cells
R1322 T2211 T2210 punct /,CbCln3Δex7
R1323 T2212 T2210 nummod 8,CbCln3Δex7
R1324 T2213 T2214 amod numerous,abnormalities
R1325 T2214 T2193 dobj abnormalities,displayed
R1326 T2215 T2214 amod ultrastructural,abnormalities
R1327 T2216 T2214 prep including,abnormalities
R1328 T2217 T2218 npadvmod electron,dense
R1329 T2218 T2219 amod dense,inclusions
R1330 T2219 T2216 pobj inclusions,including
R1331 T2220 T2219 amod characteristic,inclusions
R1332 T2221 T2220 prep of,characteristic
R1333 T2222 T2221 pobj lipofuscin,of
R1334 T2223 T2219 cc and,inclusions
R1335 T2224 T2225 amod large,autophagosomes
R1336 T2225 T2219 conj autophagosomes,inclusions
R1337 T2226 T2227 dep that,contained
R1338 T2227 T2225 relcl contained,autophagosomes
R1339 T2228 T2229 amod dense,structures
R1340 T2229 T2227 dobj structures,contained
R1341 T2230 T2229 compound core,structures
R1342 T2231 T2229 punct ", ",structures
R1343 T2232 T2233 amod degenerating,mitochondria
R1344 T2233 T2229 conj mitochondria,structures
R1345 T2234 T2233 punct ", ",mitochondria
R1346 T2235 T2233 cc and,mitochondria
R1347 T2236 T2237 amod many,vesicles
R1348 T2237 T2233 conj vesicles,mitochondria
R1349 T2238 T2237 amod smaller,vesicles
R1350 T2239 T2240 punct (,4c
R1351 T2240 T2227 parataxis 4c,contained
R1352 T2241 T2240 compound Fig.,4c
R1353 T2242 T2240 punct ),4c
R1354 T2243 T2193 punct .,displayed
R1355 T2245 T2246 compound Inclusion,bodies
R1356 T2246 T2247 nsubjpass bodies,observed
R1357 T2248 T2246 cc and,bodies
R1358 T2249 T2246 conj autophagosomes,bodies
R1359 T2250 T2247 auxpass were,observed
R1360 T2251 T2247 advmod infrequently,observed
R1361 T2252 T2247 prep in,observed
R1362 T2253 T2254 npadvmod confluency,aged
R1363 T2254 T2256 amod aged,cultures
R1364 T2255 T2254 punct -,aged
R1365 T2256 T2252 pobj cultures,in
R1366 T2257 T2258 amod wild,type
R1367 T2258 T2256 compound type,cultures
R1368 T2259 T2258 punct -,type
R1369 T2260 T2261 punct (,shown
R1370 T2261 T2247 parataxis shown,observed
R1371 T2262 T2261 nsubj data,shown
R1372 T2263 T2261 neg not,shown
R1373 T2264 T2261 punct ),shown
R1374 T2265 T2247 punct .,observed
R5202 T8215 T8216 compound RT,PCR
R5203 T8216 T8218 dep PCR,shown
R5204 T8217 T8216 punct -,PCR
R5205 T8219 T8216 prep of,PCR
R5206 T8220 T8221 compound Cln3,mRNA
R5207 T8221 T8219 pobj mRNA,of
R5208 T8222 T8216 prep in,PCR
R5209 T8223 T8224 amod wild,type
R5210 T8224 T8226 nmod type,cells
R5211 T8225 T8224 punct -,type
R5212 T8226 T8222 pobj cells,in
R5213 T8227 T8224 cc and,type
R5214 T8228 T8224 conj homozygous,type
R5215 T8229 T8226 nmod CbCln3Δex7,cells
R5216 T8230 T8229 punct /,CbCln3Δex7
R5217 T8231 T8229 nummod 8,CbCln3Δex7
R5218 T8232 T8233 nmod Cln3,products
R5219 T8233 T8218 nsubjpass products,shown
R5220 T8234 T8235 npadvmod Exon1,forward
R5221 T8235 T8237 amod forward,reverse
R5222 T8236 T8235 punct -,forward
R5223 T8237 T8233 nmod reverse,products
R5224 T8238 T8237 punct ", ",reverse
R5225 T8239 T8237 npadvmod Exon,reverse
R5226 T8240 T8239 nummod 15,Exon
R5227 T8241 T8237 punct -,reverse
R5228 T8242 T8243 compound RT,PCR
R5229 T8243 T8233 compound PCR,products
R5230 T8244 T8243 punct -,PCR
R5231 T8245 T8218 auxpass are,shown
R5232 T8246 T8218 punct ", ",shown
R5233 T8247 T8218 prep from,shown
R5234 T8248 T8249 amod total,RNA
R5235 T8249 T8247 pobj RNA,from
R5236 T8250 T8251 amod wild,type
R5237 T8251 T8249 nmod type,RNA
R5238 T8252 T8251 punct -,type
R5239 T8253 T8251 punct (,type
R5240 T8254 T8255 punct +,+
R5241 T8255 T8251 appos +,type
R5242 T8256 T8255 punct /,+
R5243 T8257 T8251 punct ),type
R5244 T8258 T8251 cc or,type
R5245 T8259 T8260 amod homozygous,mutant
R5246 T8260 T8251 conj mutant,type
R5247 T8261 T8260 punct (,mutant
R5248 T8262 T8260 appos Δex7,mutant
R5249 T8263 T8262 punct /,Δex7
R5250 T8264 T8262 nummod 8,Δex7
R5251 T8265 T8262 punct /,Δex7
R5252 T8266 T8262 appos Δex7,Δex7
R5253 T8267 T8266 punct /,Δex7
R5254 T8268 T8266 nummod 8,Δex7
R5255 T8269 T8249 punct ),RNA
R5256 T8270 T8249 nmod brain,RNA
R5257 T8271 T8270 cc and,brain
R5258 T8272 T8273 compound cell,line
R5259 T8273 T8270 conj line,brain
R5260 T8274 T8218 punct .,shown
R5261 T8276 T8277 nmod Brain,products
R5262 T8277 T8285 nsubj products,had
R5263 T8278 T8276 cc and,Brain
R5264 T8279 T8280 compound cell,line
R5265 T8280 T8276 conj line,Brain
R5266 T8281 T8282 compound RT,PCR
R5267 T8282 T8277 compound PCR,products
R5268 T8283 T8282 punct -,PCR
R5269 T8284 T8277 compound reaction,products
R5270 T8286 T8287 amod identical,patterns
R5271 T8287 T8285 dobj patterns,had
R5272 T8288 T8287 compound band,patterns
R5273 T8289 T8285 prep on,had
R5274 T8290 T8291 compound ethidium,bromide
R5275 T8291 T8293 npadvmod bromide,stained
R5276 T8292 T8291 punct -,bromide
R5277 T8293 T8294 amod stained,gels
R5278 T8294 T8289 pobj gels,on
R5279 T8295 T8294 compound agarose,gels
R5280 T8296 T8285 punct .,had
R5281 T8298 T8299 amod Wild,type
R5282 T8299 T8301 compound type,product
R5283 T8300 T8299 punct -,type
R5284 T8301 T8305 nsubj product,was
R5285 T8302 T8303 compound RT,PCR
R5286 T8303 T8301 compound PCR,product
R5287 T8304 T8303 punct -,PCR
R5288 T8306 T8307 det a,band
R5289 T8307 T8305 attr band,was
R5290 T8308 T8307 amod single,band
R5291 T8309 T8310 punct ~,1.6
R5292 T8310 T8311 nummod 1.6,kb
R5293 T8311 T8307 compound kb,band
R5294 T8312 T8305 cc and,was
R5295 T8313 T8314 compound mutant,products
R5296 T8314 T8315 nsubj products,were
R5297 T8315 T8305 conj were,was
R5298 T8316 T8317 punct ~,1.6
R5299 T8317 T8318 nummod 1.6,kb
R5300 T8318 T8315 attr kb,were
R5301 T8319 T8317 punct ", ",1.6
R5302 T8320 T8321 punct ~,1.5
R5303 T8321 T8317 conj 1.5,1.6
R5304 T8322 T8321 punct ", ",1.5
R5305 T8323 T8324 punct ~,1.4
R5306 T8324 T8321 conj 1.4,1.5
R5307 T8325 T8324 punct ", ",1.4
R5308 T8326 T8327 punct ~,1.35
R5309 T8327 T8324 conj 1.35,1.4
R5310 T8328 T8327 punct ", ",1.35
R5311 T8329 T8327 cc and,1.35
R5312 T8330 T8331 punct ~,1.3
R5313 T8331 T8327 conj 1.3,1.35
R5314 T8332 T8315 punct ", ",were
R5315 T8333 T8315 advcl representing,were
R5316 T8334 T8335 amod multiple,variants
R5317 T8335 T8333 dobj variants,representing
R5318 T8336 T8337 compound mutant,splice
R5319 T8337 T8335 compound splice,variants
R5320 T8338 T8315 punct .,were
R5321 T8445 T8446 nmod Battenin,marker
R5322 T8446 T8451 compound marker,co-staining
R5323 T8447 T8445 cc and,Battenin
R5324 T8448 T8445 conj lysosomal,Battenin
R5325 T8449 T8448 cc and,lysosomal
R5326 T8450 T8448 conj endosomal,lysosomal
R5327 T8451 T8452 dep co-staining,shown
R5328 T8453 T8451 prep in,co-staining
R5329 T8454 T8455 amod wild,type
R5330 T8455 T8457 nmod type,cells
R5331 T8456 T8455 punct -,type
R5332 T8457 T8453 pobj cells,in
R5333 T8458 T8455 cc and,type
R5334 T8459 T8455 conj homozygous,type
R5335 T8460 T8461 nmod CbCln3Δex7,precursor
R5336 T8461 T8457 compound precursor,cells
R5337 T8462 T8460 punct /,CbCln3Δex7
R5338 T8463 T8460 nummod 8,CbCln3Δex7
R5339 T8464 T8461 amod cerebellar,precursor
R5340 T8465 T8466 compound Batp1,immunostaining
R5341 T8466 T8452 nsubjpass immunostaining,shown
R5342 T8467 T8466 prep of,immunostaining
R5343 T8468 T8469 amod wild,type
R5344 T8469 T8467 pobj type,of
R5345 T8470 T8469 punct -,type
R5346 T8471 T8469 punct (,type
R5347 T8472 T8469 appos CbCln3,type
R5348 T8473 T8472 punct +,CbCln3
R5349 T8474 T8472 punct /,CbCln3
R5350 T8475 T8472 punct +,CbCln3
R5351 T8476 T8469 punct ),type
R5352 T8477 T8469 cc and,type
R5353 T8478 T8479 amod homozygous,mutant
R5354 T8479 T8480 nmod mutant,cells
R5355 T8480 T8469 conj cells,type
R5356 T8481 T8479 punct (,mutant
R5357 T8482 T8483 nmod CbCln3Δex7,Δex7
R5358 T8483 T8479 appos Δex7,mutant
R5359 T8484 T8483 punct /,Δex7
R5360 T8485 T8483 nummod 8,Δex7
R5361 T8486 T8483 punct /,Δex7
R5362 T8487 T8483 punct /,Δex7
R5363 T8488 T8483 nummod 8,Δex7
R5364 T8489 T8480 punct ),cells
R5365 T8490 T8480 amod cerebellar,cells
R5366 T8491 T8480 compound precursor,cells
R5367 T8492 T8452 auxpass is,shown
R5368 T8493 T8452 punct ", ",shown
R5369 T8494 T8452 prep with,shown
R5370 T8495 T8494 pobj co-staining,with
R5371 T8496 T8495 prep for,co-staining
R5372 T8497 T8496 pobj lysosomes,for
R5373 T8498 T8497 punct (,lysosomes
R5374 T8499 T8497 appos Lamp,lysosomes
R5375 T8500 T8499 nummod 1,Lamp
R5376 T8501 T8497 punct ),lysosomes
R5377 T8502 T8497 punct ", ",lysosomes
R5378 T8503 T8504 amod early,endosomes
R5379 T8504 T8497 conj endosomes,lysosomes
R5380 T8505 T8504 punct (,endosomes
R5381 T8506 T8504 appos EEA1,endosomes
R5382 T8507 T8504 punct ),endosomes
R5383 T8508 T8504 punct ", ",endosomes
R5384 T8509 T8504 cc and,endosomes
R5385 T8510 T8511 amod late,endosomes
R5386 T8511 T8504 conj endosomes,endosomes
R5387 T8512 T8511 punct (,endosomes
R5388 T8513 T8511 appos Rab7,endosomes
R5389 T8514 T8452 punct ),shown
R5390 T8515 T8452 punct .,shown
R5391 T8517 T8518 amod Significant,overlap
R5392 T8518 T8519 nsubjpass overlap,seen
R5393 T8520 T8518 prep of,overlap
R5394 T8521 T8522 compound Batp1,signal
R5395 T8522 T8520 pobj signal,of
R5396 T8523 T8524 punct (,red
R5397 T8524 T8522 parataxis red,signal
R5398 T8525 T8524 punct ),red
R5399 T8526 T8518 prep with,overlap
R5400 T8527 T8526 pobj EEA1,with
R5401 T8528 T8529 punct (,panels
R5402 T8529 T8527 parataxis panels,EEA1
R5403 T8530 T8529 amod green,panels
R5404 T8531 T8529 punct ", ",panels
R5405 T8532 T8529 amod middle,panels
R5406 T8533 T8529 punct ),panels
R5407 T8534 T8527 cc and,EEA1
R5408 T8535 T8527 conj Rab7,EEA1
R5409 T8536 T8537 punct (,panels
R5410 T8537 T8535 parataxis panels,Rab7
R5411 T8538 T8537 amod green,panels
R5412 T8539 T8537 punct ", ",panels
R5413 T8540 T8537 amod bottom,panels
R5414 T8541 T8537 punct ),panels
R5415 T8542 T8519 aux can,seen
R5416 T8543 T8519 auxpass be,seen
R5417 T8544 T8519 prep as,seen
R5418 T8545 T8544 amod yellow,as
R5419 T8546 T8547 advmod when,merged
R5420 T8547 T8519 advcl merged,seen
R5421 T8548 T8549 det the,channels
R5422 T8549 T8547 nsubjpass channels,merged
R5423 T8550 T8549 nummod two,channels
R5424 T8551 T8547 auxpass are,merged
R5425 T8552 T8553 punct (,Merge
R5426 T8553 T8547 parataxis Merge,merged
R5427 T8554 T8553 punct ),Merge
R5428 T8555 T8519 punct .,seen
R5429 T8557 T8558 det The,degree
R5430 T8558 T8559 nsubj degree,is
R5431 T8560 T8558 prep of,degree
R5432 T8561 T8562 compound Batp1,overlap
R5433 T8562 T8560 pobj overlap,of
R5434 T8563 T8559 acomp greatest,is
R5435 T8564 T8559 prep with,is
R5436 T8565 T8564 pobj Rab7,with
R5437 T8566 T8559 punct .,is
R5438 T8568 T8569 advmod Only,overlap
R5439 T8569 T8571 nsubjpass overlap,seen
R5440 T8570 T8569 amod limited,overlap
R5441 T8572 T8569 prep between,overlap
R5442 T8573 T8572 pobj Batp1,between
R5443 T8574 T8575 punct (,red
R5444 T8575 T8573 parataxis red,Batp1
R5445 T8576 T8575 punct ),red
R5446 T8577 T8573 cc and,Batp1
R5447 T8578 T8573 conj Lamp,Batp1
R5448 T8579 T8578 nummod 1,Lamp
R5449 T8580 T8581 punct (,panels
R5450 T8581 T8578 parataxis panels,Lamp
R5451 T8582 T8581 amod green,panels
R5452 T8583 T8581 punct ", ",panels
R5453 T8584 T8581 amod top,panels
R5454 T8585 T8581 punct ),panels
R5455 T8586 T8571 aux can,seen
R5456 T8587 T8571 auxpass be,seen
R5457 T8588 T8571 punct .,seen
R5458 T8590 T8591 compound Batp1,signal
R5459 T8591 T8592 nsubj signal,is
R5460 T8593 T8591 prep in,signal
R5461 T8594 T8595 amod homozygous,cells
R5462 T8595 T8593 pobj cells,in
R5463 T8596 T8595 nmod CbCln3Δex7,cells
R5464 T8597 T8596 punct /,CbCln3Δex7
R5465 T8598 T8596 nummod 8,CbCln3Δex7
R5466 T8599 T8600 advmod significantly,reduced
R5467 T8600 T8592 acomp reduced,is
R5468 T8601 T8592 punct ", ",is
R5469 T8602 T8592 cc but,is
R5470 T8603 T8604 amod significant,overlap
R5471 T8604 T8605 nsubjpass overlap,seen
R5472 T8605 T8592 conj seen,is
R5473 T8606 T8604 prep with,overlap
R5474 T8607 T8606 pobj EEA1,with
R5475 T8608 T8607 cc and,EEA1
R5476 T8609 T8607 conj Rab7,EEA1
R5477 T8610 T8604 punct ", ",overlap
R5478 T8611 T8604 cc and,overlap
R5479 T8612 T8613 advmod very,little
R5480 T8613 T8614 amod little,overlap
R5481 T8614 T8604 conj overlap,overlap
R5482 T8615 T8614 nmod Lamp,overlap
R5483 T8616 T8615 nummod 1,Lamp
R5484 T8617 T8605 punct ", ",seen
R5485 T8618 T8605 aux can,seen
R5486 T8619 T8605 auxpass be,seen
R5487 T8620 T8605 prep as,seen
R5488 T8621 T8620 amod yellow,as
R5489 T8622 T8605 prep in,seen
R5490 T8623 T8624 det the,panels
R5491 T8624 T8622 pobj panels,in
R5492 T8625 T8624 amod respective,panels
R5493 T8626 T8624 amod merged,panels
R5494 T8627 T8605 punct .,seen
R5495 T8629 T8630 advmod Notably,appear
R5496 T8631 T8630 punct ", ",appear
R5497 T8632 T8633 nmod Lamp,localization
R5498 T8633 T8630 nsubj localization,appear
R5499 T8634 T8632 nummod 1,Lamp
R5500 T8635 T8632 cc and,Lamp
R5501 T8636 T8632 conj EEA1,Lamp
R5502 T8637 T8630 oprd altered,appear
R5503 T8638 T8630 punct ", ",appear
R5504 T8639 T8630 cc and,appear
R5505 T8640 T8641 compound Rab7,staining
R5506 T8641 T8642 nsubj staining,was
R5507 T8642 T8630 conj was,appear
R5508 T8643 T8642 advmod frequently,was
R5509 T8644 T8645 advmod less,intense
R5510 T8645 T8642 acomp intense,was
R5511 T8646 T8642 prep in,was
R5512 T8647 T8648 amod homozygous,cells
R5513 T8648 T8646 pobj cells,in
R5514 T8649 T8648 nmod CbCln3Δex7,cells
R5515 T8650 T8649 punct /,CbCln3Δex7
R5516 T8651 T8649 nummod 8,CbCln3Δex7
R5517 T8652 T8642 punct .,was
R5518 T8654 T8655 amod Wild,type
R5519 T8655 T8657 nsubjpass type,captured
R5520 T8656 T8655 punct -,type
R5521 T8658 T8655 cc and,type
R5522 T8659 T8660 amod homozygous,CbCln3Δex7
R5523 T8660 T8661 nmod CbCln3Δex7,images
R5524 T8661 T8655 conj images,type
R5525 T8662 T8660 punct /,CbCln3Δex7
R5526 T8663 T8660 nummod 8,CbCln3Δex7
R5527 T8664 T8661 amod confocal,images
R5528 T8665 T8657 auxpass were,captured
R5529 T8666 T8657 prep with,captured
R5530 T8667 T8668 amod identical,settings
R5531 T8668 T8666 pobj settings,with
R5532 T8669 T8668 compound exposure,settings
R5533 T8670 T8657 punct .,captured
R5534 T8672 T8673 nummod 60,magnification
R5535 T8674 T8672 punct ×,60
R5536 T8675 T8673 punct .,magnification
R5537 T8805 T8806 compound Subunit,c
R5538 T8806 T8807 compound c,accumulation
R5539 T8808 T8807 prep in,accumulation
R5540 T8809 T8810 amod homozygous,cells
R5541 T8810 T8808 pobj cells,in
R5542 T8811 T8810 nmod CbCln3Δex7,cells
R5543 T8812 T8811 punct /,CbCln3Δex7
R5544 T8813 T8811 nummod 8,CbCln3Δex7
R5545 T8814 T8815 amod cerebellar,precursor
R5546 T8815 T8810 compound precursor,cells
R5547 T8817 T8818 meta a,shown
R5548 T8819 T8817 punct .,a
R5549 T8820 T8821 compound Subunit,c
R5550 T8821 T8818 nsubjpass c,shown
R5551 T8822 T8821 appos immunostaining,c
R5552 T8823 T8822 cc and,immunostaining
R5553 T8824 T8822 conj autofluorescence,immunostaining
R5554 T8825 T8821 prep of,c
R5555 T8826 T8827 nummod 7,day
R5556 T8827 T8829 compound day,confluency
R5557 T8828 T8827 punct -,day
R5558 T8829 T8830 npadvmod confluency,aged
R5559 T8830 T8832 amod aged,cells
R5560 T8831 T8830 punct -,aged
R5561 T8832 T8825 pobj cells,of
R5562 T8833 T8834 amod wild,type
R5563 T8834 T8832 nmod type,cells
R5564 T8835 T8834 punct -,type
R5565 T8836 T8834 cc and,type
R5566 T8837 T8834 conj homozygous,type
R5567 T8838 T8832 nmod CbCln3Δex7,cells
R5568 T8839 T8838 punct /,CbCln3Δex7
R5569 T8840 T8838 nummod 8,CbCln3Δex7
R5570 T8841 T8818 auxpass is,shown
R5571 T8842 T8818 punct .,shown
R5572 T8844 T8845 amod Wild,type
R5573 T8845 T8847 compound type,cultures
R5574 T8846 T8845 punct -,type
R5575 T8847 T8848 nsubj cultures,exhibited
R5576 T8849 T8847 punct (,cultures
R5577 T8850 T8847 appos CbCln3,cultures
R5578 T8851 T8850 punct +,CbCln3
R5579 T8852 T8850 punct /,CbCln3
R5580 T8853 T8850 punct +,CbCln3
R5581 T8854 T8848 punct ),exhibited
R5582 T8855 T8856 amod limited,c
R5583 T8856 T8848 dobj c,exhibited
R5584 T8857 T8856 compound subunit,c
R5585 T8858 T8856 appos immunostaining,c
R5586 T8859 T8858 cc and,immunostaining
R5587 T8860 T8858 conj autofluorescence,immunostaining
R5588 T8861 T8848 punct .,exhibited
R5589 T8863 T8864 advmod However,contained
R5590 T8865 T8864 punct ", ",contained
R5591 T8866 T8867 nmod CbCln3Δex7,Δex7
R5592 T8867 T8871 nmod Δex7,cells
R5593 T8868 T8867 punct /,Δex7
R5594 T8869 T8867 nummod 8,Δex7
R5595 T8870 T8867 punct /,Δex7
R5596 T8871 T8864 nsubj cells,contained
R5597 T8872 T8867 punct /,Δex7
R5598 T8873 T8867 nummod 8,Δex7
R5599 T8874 T8875 amod numerous,puncta
R5600 T8875 T8864 dobj puncta,contained
R5601 T8876 T8877 compound subunit,c
R5602 T8877 T8875 compound c,puncta
R5603 T8878 T8864 punct .,contained
R5604 T8880 T8881 nsubj Autofluorescence,was
R5605 T8882 T8883 punct (,AF
R5606 T8883 T8880 parataxis AF,Autofluorescence
R5607 T8884 T8885 nummod 7,days
R5608 T8885 T8883 compound days,AF
R5609 T8886 T8883 punct ),AF
R5610 T8887 T8881 advmod also,was
R5611 T8888 T8889 advmod significantly,elevated
R5612 T8889 T8881 acomp elevated,was
R5613 T8890 T8891 punct (,panels
R5614 T8891 T8889 parataxis panels,elevated
R5615 T8892 T8891 amod right,panels
R5616 T8893 T8891 punct ),panels
R5617 T8894 T8881 punct ", ",was
R5618 T8895 T8896 mark although,observed
R5619 T8896 T8881 advcl observed,was
R5620 T8897 T8898 amod limited,overlap
R5621 T8898 T8896 nsubjpass overlap,observed
R5622 T8899 T8898 prep with,overlap
R5623 T8900 T8901 compound subunit,c
R5624 T8901 T8902 compound c,puncta
R5625 T8902 T8899 pobj puncta,with
R5626 T8903 T8896 auxpass was,observed
R5627 T8904 T8905 punct (,arrows
R5628 T8905 T8896 parataxis arrows,observed
R5629 T8906 T8905 punct ),arrows
R5630 T8907 T8881 punct .,was
R5631 T8909 T8910 nummod 40,magnification
R5632 T8911 T8909 punct ×,40
R5633 T8912 T8910 punct .,magnification
R5634 T8914 T8915 meta b,shown
R5635 T8916 T8914 punct .,b
R5636 T8917 T8918 compound Immunoblot,analysis
R5637 T8918 T8915 nsubjpass analysis,shown
R5638 T8919 T8918 prep of,analysis
R5639 T8920 T8921 compound subunit,c
R5640 T8921 T8922 compound c,protein
R5641 T8922 T8919 pobj protein,of
R5642 T8923 T8918 prep at,analysis
R5643 T8924 T8925 nmod sub-confluency,incubation
R5644 T8925 T8923 pobj incubation,at
R5645 T8926 T8924 cc or,sub-confluency
R5646 T8927 T8928 nummod 7,day
R5647 T8928 T8930 compound day,confluency
R5648 T8929 T8928 punct -,day
R5649 T8930 T8924 conj confluency,sub-confluency
R5650 T8931 T8915 auxpass is,shown
R5651 T8932 T8915 punct .,shown
R5652 T8934 T8935 amod Total,extracts
R5653 T8935 T8937 nsubj extracts,contained
R5654 T8936 T8935 compound protein,extracts
R5655 T8938 T8935 prep from,extracts
R5656 T8939 T8940 nmod sub-confluency,cultures
R5657 T8940 T8938 pobj cultures,from
R5658 T8941 T8942 amod wild,type
R5659 T8942 T8940 nmod type,cultures
R5660 T8943 T8942 punct -,type
R5661 T8944 T8942 punct (,type
R5662 T8945 T8946 punct +,+
R5663 T8946 T8942 appos +,type
R5664 T8947 T8946 punct /,+
R5665 T8948 T8942 punct ),type
R5666 T8949 T8942 cc and,type
R5667 T8950 T8951 amod homozygous,mutant
R5668 T8951 T8942 conj mutant,type
R5669 T8952 T8951 punct (,mutant
R5670 T8953 T8951 appos Δex7,mutant
R5671 T8954 T8953 punct /,Δex7
R5672 T8955 T8953 nummod 8,Δex7
R5673 T8956 T8953 punct /,Δex7
R5674 T8957 T8953 appos Δex7,Δex7
R5675 T8958 T8957 punct /,Δex7
R5676 T8959 T8957 nummod 8,Δex7
R5677 T8960 T8940 punct ),cultures
R5678 T8961 T8962 advmod approximately,equal
R5679 T8962 T8963 amod equal,levels
R5680 T8963 T8937 dobj levels,contained
R5681 T8964 T8963 prep of,levels
R5682 T8965 T8966 compound subunit,c
R5683 T8966 T8967 compound c,protein
R5684 T8967 T8964 pobj protein,of
R5685 T8968 T8967 punct (,protein
R5686 T8969 T8970 nmod α,c
R5687 T8970 T8967 appos c,protein
R5688 T8971 T8969 punct -,α
R5689 T8972 T8969 amod sub,α
R5690 T8973 T8937 punct ),contained
R5691 T8974 T8937 punct .,contained
R5692 T8976 T8977 nummod 7,day
R5693 T8977 T8979 compound day,confluency
R5694 T8978 T8977 punct -,day
R5695 T8979 T8980 compound confluency,extract
R5696 T8980 T8981 nsubj extract,had
R5697 T8982 T8980 prep from,extract
R5698 T8983 T8984 amod homozygous,cells
R5699 T8984 T8982 pobj cells,from
R5700 T8985 T8984 nmod CbCln3Δex7,cells
R5701 T8986 T8985 punct /,CbCln3Δex7
R5702 T8987 T8985 nummod 8,CbCln3Δex7
R5703 T8988 T8984 punct (,cells
R5704 T8989 T8984 appos Δex7,cells
R5705 T8990 T8989 punct /,Δex7
R5706 T8991 T8989 nummod 8,Δex7
R5707 T8992 T8989 punct /,Δex7
R5708 T8993 T8989 appos Δex7,Δex7
R5709 T8994 T8993 punct /,Δex7
R5710 T8995 T8993 nummod 8,Δex7
R5711 T8996 T8981 punct ),had
R5712 T8997 T8998 amod elevated,levels
R5713 T8998 T8981 dobj levels,had
R5714 T8999 T8998 prep of,levels
R5715 T9000 T9001 compound subunit,c
R5716 T9001 T9002 compound c,protein
R5717 T9002 T8999 pobj protein,of
R5718 T9003 T8998 punct (,levels
R5719 T9004 T9005 punct ~,1.5
R5720 T9005 T8998 npadvmod 1.5,levels
R5721 T9006 T9005 punct X,1.5
R5722 T9007 T8998 punct ),levels
R5723 T9008 T8998 punct ", ",levels
R5724 T9009 T8998 amod relative,levels
R5725 T9010 T9009 prep to,relative
R5726 T9011 T9012 amod wild,type
R5727 T9012 T9014 compound type,extract
R5728 T9013 T9012 punct -,type
R5729 T9014 T9010 pobj extract,to
R5730 T9015 T9014 punct (,extract
R5731 T9016 T9017 punct +,+
R5732 T9017 T9014 appos +,extract
R5733 T9018 T9017 punct /,+
R5734 T9019 T8981 punct ),had
R5735 T9020 T8981 punct .,had
R5736 T9022 T9023 compound Protein,levels
R5737 T9023 T9024 nsubjpass levels,normalized
R5738 T9025 T9024 auxpass were,normalized
R5739 T9026 T9024 prep to,normalized
R5740 T9027 T9028 compound cytochrome,c
R5741 T9028 T9029 compound c,oxidase
R5742 T9029 T9030 compound oxidase,subunit
R5743 T9030 T9026 pobj subunit,to
R5744 T9031 T9030 nummod IV,subunit
R5745 T9032 T9030 punct (,subunit
R5746 T9033 T9034 compound α,cox4
R5747 T9034 T9030 appos cox4,subunit
R5748 T9035 T9034 punct -,cox4
R5749 T9036 T9024 punct ),normalized
R5750 T9037 T9024 punct .,normalized
R5751 T9039 T9040 meta c,shown
R5752 T9041 T9039 punct .,c
R5753 T9042 T9043 compound TEM,analysis
R5754 T9043 T9040 nsubjpass analysis,shown
R5755 T9044 T9043 prep of,analysis
R5756 T9045 T9044 pobj inclusions,of
R5757 T9046 T9043 prep in,analysis
R5758 T9047 T9048 nummod 7,day
R5759 T9048 T9050 compound day,confluency
R5760 T9049 T9048 punct -,day
R5761 T9050 T9051 npadvmod confluency,aged
R5762 T9051 T9053 amod aged,cells
R5763 T9052 T9051 punct -,aged
R5764 T9053 T9046 pobj cells,in
R5765 T9054 T9053 amod homozygous,cells
R5766 T9055 T9053 nmod CbCln3Δex7,cells
R5767 T9056 T9055 punct /,CbCln3Δex7
R5768 T9057 T9055 nummod 8,CbCln3Δex7
R5769 T9058 T9040 auxpass is,shown
R5770 T9059 T9040 punct .,shown
R5771 T9061 T9062 det A,autophagosome
R5772 T9062 T9064 nsubjpass autophagosome,filled
R5773 T9063 T9062 amod large,autophagosome
R5774 T9065 T9062 acl contained,autophagosome
R5775 T9066 T9065 agent by,contained
R5776 T9067 T9068 amod double,membrane
R5777 T9068 T9066 pobj membrane,by
R5778 T9069 T9070 punct (,arrows
R5779 T9070 T9065 parataxis arrows,contained
R5780 T9071 T9070 punct ),arrows
R5781 T9072 T9064 auxpass is,filled
R5782 T9073 T9064 prep with,filled
R5783 T9074 T9075 amod degenerating,mitochondria
R5784 T9075 T9073 pobj mitochondria,with
R5785 T9076 T9075 punct (,mitochondria
R5786 T9077 T9075 appos Md,mitochondria
R5787 T9078 T9075 punct ),mitochondria
R5788 T9079 T9075 punct ", ",mitochondria
R5789 T9080 T9081 nmod electron,cores
R5790 T9081 T9075 conj cores,mitochondria
R5791 T9082 T9081 amod dense,cores
R5792 T9083 T9084 punct (,left
R5793 T9084 T9081 parataxis left,cores
R5794 T9085 T9084 cc and,left
R5795 T9086 T9084 conj right,left
R5796 T9087 T9084 prep of,left
R5797 T9088 T9087 punct *,of
R5798 T9089 T9084 punct ),left
R5799 T9090 T9081 cc and,cores
R5800 T9091 T9092 amod other,structures
R5801 T9092 T9081 conj structures,cores
R5802 T9093 T9092 amod smaller,structures
R5803 T9094 T9092 amod vesicular,structures
R5804 T9095 T9064 punct .,filled
R5805 T9097 T9098 det A,inclusion
R5806 T9098 T9103 nsubj inclusion,is
R5807 T9099 T9098 amod large,inclusion
R5808 T9100 T9101 npadvmod electron,dense
R5809 T9101 T9098 amod dense,inclusion
R5810 T9102 T9101 punct -,dense
R5811 T9104 T9098 punct ", ",inclusion
R5812 T9105 T9098 prep with,inclusion
R5813 T9106 T9107 det a,appearance
R5814 T9107 T9105 pobj appearance,with
R5815 T9108 T9107 nmod lipofuscin,appearance
R5816 T9109 T9108 punct (,lipofuscin
R5817 T9110 T9108 appos Ln,lipofuscin
R5818 T9111 T9107 punct ),appearance
R5819 T9112 T9103 punct ", ",is
R5820 T9113 T9103 advmod also,is
R5821 T9114 T9103 acomp present,is
R5822 T9115 T9103 punct .,is
R5823 T9118 T9117 punct ", ",M
R5824 T9119 T9117 appos mitochondria,M
R5825 T9120 T9117 punct .,M
R5826 T9122 T9123 nummod "10,000",magnification
R5827 T9124 T9122 punct ×,"10,000"
R5828 T9125 T9123 punct .,magnification
R913 T1775 T1776 amod Homozygous,cells
R914 T1776 T1780 nsubj cells,express
R915 T1777 T1776 nmod CbCln3Δex7,cells
R916 T1778 T1777 punct /,CbCln3Δex7
R917 T1779 T1777 nummod 8,CbCln3Δex7
R918 T1781 T1782 compound mutant,battenin
R919 T1782 T1780 dobj battenin,express
R920 T1783 T1780 cc and,express
R921 T1784 T1780 conj display,express
R922 T1785 T1786 npadvmod JNCL,like
R923 T1786 T1788 amod like,pathology
R924 T1787 T1786 punct -,like
R925 T1788 T1784 dobj pathology,display
R926 T1790 T1791 amod Homozygous,cells
R927 T1791 T1796 nsubjpass cells,examined
R928 T1792 T1793 nmod Cb,Cln3Δex7
R929 T1793 T1791 nmod Cln3Δex7,cells
R930 T1794 T1793 punct /,Cln3Δex7
R931 T1795 T1793 nummod 8,Cln3Δex7
R932 T1797 T1796 auxpass were,examined
R933 T1798 T1796 advmod first,examined
R934 T1799 T1796 prep for,examined
R935 T1800 T1801 npadvmod JNCL,like
R936 T1801 T1803 amod like,characteristics
R937 T1802 T1801 punct -,like
R938 T1803 T1799 pobj characteristics,for
R939 T1804 T1796 punct .,examined
R940 T1806 T1807 amod Homozygous,mice
R941 T1807 T1814 nsubj mice,express
R942 T1808 T1807 nmod Cln3Δex7,mice
R943 T1809 T1808 punct /,Cln3Δex7
R944 T1810 T1808 nummod 8,Cln3Δex7
R945 T1811 T1807 amod knock,mice
R946 T1812 T1811 punct -,knock
R947 T1813 T1811 prt in,knock
R948 T1815 T1816 amod multiple,variants
R949 T1816 T1814 dobj variants,express
R950 T1817 T1818 compound Cln3,splice
R951 T1818 T1816 compound splice,variants
R952 T1819 T1818 compound mRNA,splice
R953 T1820 T1816 cc and,variants
R954 T1821 T1822 compound mutant,protein
R955 T1822 T1816 conj protein,variants
R956 T1823 T1822 compound battenin,protein
R957 T1824 T1825 dep that,is
R958 T1825 T1822 relcl is,protein
R959 T1826 T1825 acomp detectable,is
R960 T1827 T1825 prep by,is
R961 T1828 T1829 compound batp1,antibody
R962 T1829 T1830 npadvmod antibody,recognizing
R963 T1830 T1831 amod recognizing,epitopes
R964 T1831 T1827 pobj epitopes,by
R965 T1832 T1833 npadvmod C,terminal
R966 T1833 T1831 amod terminal,epitopes
R967 T1834 T1833 punct -,terminal
R968 T1835 T1836 punct [,12
R969 T1836 T1814 parataxis 12,express
R970 T1837 T1836 punct ],12
R971 T1838 T1814 punct .,express
R972 T1840 T1841 aux To,assess
R973 T1841 T1842 advcl assess,performed
R974 T1843 T1844 det this,phenotype
R975 T1844 T1841 dobj phenotype,assess
R976 T1845 T1844 amod molecular,phenotype
R977 T1846 T1841 prep in,assess
R978 T1847 T1848 nmod CbCln3Δex7,cells
R979 T1848 T1846 pobj cells,in
R980 T1849 T1847 punct /,CbCln3Δex7
R981 T1850 T1847 nummod 8,CbCln3Δex7
R982 T1851 T1842 punct ", ",performed
R983 T1852 T1853 compound RT,PCR
R984 T1853 T1842 nsubjpass PCR,performed
R985 T1854 T1853 punct -,PCR
R986 T1855 T1853 cc and,PCR
R987 T1856 T1857 nmod anti-battenin,immunostaining
R988 T1857 T1853 conj immunostaining,PCR
R989 T1858 T1856 punct (,anti-battenin
R990 T1859 T1856 appos batp1,anti-battenin
R991 T1860 T1857 punct ),immunostaining
R992 T1861 T1842 auxpass were,performed
R993 T1862 T1842 punct .,performed
R994 T1864 T1865 mark As,shown
R995 T1865 T1866 advcl shown,were
R996 T1867 T1865 prep in,shown
R997 T1868 T1867 pobj Figure,in
R998 T1869 T1868 nummod 2,Figure
R999 T1870 T1866 punct ", ",were