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PMC:539297 / 4236-5416 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1281 0-2 TO denotes To
T1282 3-11 VB denotes generate
T1284 12-13 DT denotes a
T1286 14-21 JJ denotes precise
T1287 22-29 JJ denotes genetic
T1288 29-31 , denotes ,
T1289 31-37 NN denotes neuron
T1291 37-38 HYPH denotes -
T1290 38-45 VBN denotes derived
T1292 46-50 NN denotes JNCL
T1294 51-55 NN denotes cell
T1293 56-63 NN denotes culture
T1285 64-70 NN denotes system
T1295 70-72 , denotes ,
T1296 72-74 PRP denotes we
T1283 75-87 VBD denotes immortalized
T1297 88-95 NN denotes granule
T1298 96-103 NNS denotes neurons
T1299 104-112 VBN denotes cultured
T1300 113-117 IN denotes from
T1301 118-127 JJ denotes postnatal
T1302 128-131 NN denotes day
T1304 132-133 CD denotes 4
T1305 134-135 -LRB- denotes (
T1306 135-137 NN denotes P4
T1307 137-138 -RRB- denotes )
T1303 139-148 NNS denotes cerebella
T1308 149-151 IN denotes of
T1309 152-162 JJ denotes homozygous
T1311 163-166 CC denotes and
T1312 167-179 JJ denotes heterozygous
T1313 180-188 NN denotes Cln3Δex7
T1314 188-189 HYPH denotes /
T1315 189-190 CD denotes 8
T1316 191-196 VB denotes knock
T1317 196-197 HYPH denotes -
T1318 197-199 RP denotes in
T1310 200-204 NNS denotes mice
T1319 204-206 , denotes ,
T1320 206-209 CC denotes and
T1321 210-214 JJ denotes wild
T1323 214-215 HYPH denotes -
T1322 215-219 NN denotes type
T1324 220-231 NNS denotes littermates
T1325 231-232 . denotes .
T1326 232-405 sentence denotes Primary cell cultures enriched for granule neurons were transduced with retroviral vector bearing a selection cassette and temperature-sensitive tsA58 SV40 large T antigen.
T1327 233-240 JJ denotes Primary
T1329 241-245 NN denotes cell
T1328 246-254 NNS denotes cultures
T1331 255-263 VBN denotes enriched
T1332 264-267 IN denotes for
T1333 268-275 NN denotes granule
T1334 276-283 NNS denotes neurons
T1335 284-288 VBD denotes were
T1330 289-299 VBN denotes transduced
T1336 300-304 IN denotes with
T1337 305-315 JJ denotes retroviral
T1338 316-322 NN denotes vector
T1339 323-330 VBG denotes bearing
T1340 331-332 DT denotes a
T1342 333-342 NN denotes selection
T1341 343-351 NN denotes cassette
T1343 352-355 CC denotes and
T1344 356-367 NN denotes temperature
T1346 367-368 HYPH denotes -
T1345 368-377 JJ denotes sensitive
T1348 378-383 NN denotes tsA58
T1349 384-388 NN denotes SV40
T1350 389-394 JJ denotes large
T1351 395-396 NN denotes T
T1347 397-404 NN denotes antigen
T1352 404-405 . denotes .
T1353 405-616 sentence denotes Growth in G418 containing medium at the permissive temperature (33°C) allowed for selection and isolation of multiple clonal nestin-positive (Fig. 1a), and GFAP-negative (Fig. 1b), cell lines for each genotype.
T1354 406-412 NN denotes Growth
T1356 413-415 IN denotes in
T1357 416-420 NN denotes G418
T1358 421-431 VBG denotes containing
T1359 432-438 NN denotes medium
T1360 439-441 IN denotes at
T1361 442-445 DT denotes the
T1363 446-456 JJ denotes permissive
T1362 457-468 NN denotes temperature
T1364 469-470 -LRB- denotes (
T1365 470-472 CD denotes 33
T1366 472-474 NN denotes °C
T1367 474-475 -RRB- denotes )
T1355 476-483 VBN denotes allowed
T1368 484-487 IN denotes for
T1369 488-497 NN denotes selection
T1370 498-501 CC denotes and
T1371 502-511 NN denotes isolation
T1372 512-514 IN denotes of
T1373 515-523 JJ denotes multiple
T1375 524-530 JJ denotes clonal
T1376 531-537 NN denotes nestin
T1378 537-538 HYPH denotes -
T1377 538-546 JJ denotes positive
T1379 547-548 -LRB- denotes (
T1381 548-552 NN denotes Fig.
T1380 553-555 NN denotes 1a
T1382 555-556 -RRB- denotes )
T1383 556-558 , denotes ,
T1384 558-561 CC denotes and
T1385 562-566 NN denotes GFAP
T1387 566-567 HYPH denotes -
T1386 567-575 JJ denotes negative
T1388 576-577 -LRB- denotes (
T1390 577-581 NN denotes Fig.
T1389 582-584 NN denotes 1b
T1391 584-585 -RRB- denotes )
T1392 585-587 , denotes ,
T1393 587-591 NN denotes cell
T1374 592-597 NNS denotes lines
T1394 598-601 IN denotes for
T1395 602-606 DT denotes each
T1396 607-615 NN denotes genotype
T1397 615-616 . denotes .
T1398 616-733 sentence denotes No genotype specific differences were observed in cellular morphology or doubling time (~46 hours) (data not shown).
T1399 617-619 DT denotes No
T1401 620-628 NN denotes genotype
T1402 629-637 JJ denotes specific
T1400 638-649 NNS denotes differences
T1404 650-654 VBD denotes were
T1403 655-663 VBN denotes observed
T1405 664-666 IN denotes in
T1406 667-675 JJ denotes cellular
T1407 676-686 NN denotes morphology
T1408 687-689 CC denotes or
T1409 690-698 NN denotes doubling
T1410 699-703 NN denotes time
T1411 704-705 -LRB- denotes (
T1412 705-706 SYM denotes ~
T1413 706-708 CD denotes 46
T1414 709-714 NNS denotes hours
T1415 714-715 -RRB- denotes )
T1416 716-717 -LRB- denotes (
T1418 717-721 NNS denotes data
T1419 722-725 RB denotes not
T1417 726-731 VBN denotes shown
T1420 731-732 -RRB- denotes )
T1421 732-733 . denotes .
T1422 733-903 sentence denotes As expected, SV40 large T antigen expression was rapidly lost and cell division ceased when cells were shifted to the non-permissive temperature (39°C) (data not shown).
T1423 734-736 IN denotes As
T1424 737-745 VBN denotes expected
T1426 745-747 , denotes ,
T1427 747-751 NN denotes SV40
T1429 752-757 JJ denotes large
T1430 758-759 NN denotes T
T1431 760-767 NN denotes antigen
T1428 768-778 NN denotes expression
T1432 779-782 VBD denotes was
T1433 783-790 RB denotes rapidly
T1425 791-795 VBN denotes lost
T1434 796-799 CC denotes and
T1435 800-804 NN denotes cell
T1436 805-813 NN denotes division
T1437 814-820 VBD denotes ceased
T1438 821-825 WRB denotes when
T1440 826-831 NNS denotes cells
T1441 832-836 VBD denotes were
T1439 837-844 VBN denotes shifted
T1442 845-847 IN denotes to
T1443 848-851 DT denotes the
T1445 852-866 JJ denotes non-permissive
T1444 867-878 NN denotes temperature
T1446 879-880 -LRB- denotes (
T1447 880-882 CD denotes 39
T1448 882-884 NN denotes °C
T1449 884-885 -RRB- denotes )
T1450 886-887 -LRB- denotes (
T1452 887-891 NNS denotes data
T1453 892-895 RB denotes not
T1451 896-901 VBN denotes shown
T1454 901-902 -RRB- denotes )
T1455 902-903 . denotes .
T1456 903-1180 sentence denotes Upon addition of neuronal differentiation cocktail, precursor cells became neuron-like in morphology and exhibited decreased nestin expression (data not shown) and increased MAP2 and NeuN expression (Fig. 1c,1d), but not expression of the Purkinje marker, calbindin (Fig. 1e).
T1457 904-908 IN denotes Upon
T1459 909-917 NN denotes addition
T1460 918-920 IN denotes of
T1461 921-929 JJ denotes neuronal
T1462 930-945 NN denotes differentiation
T1463 946-954 NN denotes cocktail
T1464 954-956 , denotes ,
T1465 956-965 NN denotes precursor
T1466 966-971 NNS denotes cells
T1458 972-978 VBD denotes became
T1467 979-985 NN denotes neuron
T1469 985-986 HYPH denotes -
T1468 986-990 JJ denotes like
T1470 991-993 IN denotes in
T1471 994-1004 NN denotes morphology
T1472 1005-1008 CC denotes and
T1473 1009-1018 VBD denotes exhibited
T1474 1019-1028 VBN denotes decreased
T1476 1029-1035 NN denotes nestin
T1475 1036-1046 NN denotes expression
T1477 1047-1048 -LRB- denotes (
T1479 1048-1052 NNS denotes data
T1480 1053-1056 RB denotes not
T1478 1057-1062 VBN denotes shown
T1481 1062-1063 -RRB- denotes )
T1482 1064-1067 CC denotes and
T1483 1068-1077 VBN denotes increased
T1485 1078-1082 NN denotes MAP2
T1486 1083-1086 CC denotes and
T1487 1087-1091 NN denotes NeuN
T1484 1092-1102 NN denotes expression
T1488 1103-1104 -LRB- denotes (
T1490 1104-1108 NN denotes Fig.
T1491 1109-1111 NN denotes 1c
T1492 1111-1112 , denotes ,
T1489 1112-1114 NN denotes 1d
T1493 1114-1115 -RRB- denotes )
T1494 1115-1117 , denotes ,
T1495 1117-1120 CC denotes but
T1496 1121-1124 RB denotes not
T1497 1125-1135 NN denotes expression
T1498 1136-1138 IN denotes of
T1499 1139-1142 DT denotes the
T1501 1143-1151 NNP denotes Purkinje
T1500 1152-1158 NN denotes marker
T1502 1158-1160 , denotes ,
T1503 1160-1169 NN denotes calbindin
T1504 1170-1171 -LRB- denotes (
T1506 1171-1175 NN denotes Fig.
T1505 1176-1178 NN denotes 1e
T1507 1178-1179 -RRB- denotes )
T1508 1179-1180 . denotes .
R696 T1281 T1282 aux To,generate
R697 T1282 T1283 advcl generate,immortalized
R698 T1284 T1285 det a,system
R699 T1285 T1282 dobj system,generate
R700 T1286 T1285 amod precise,system
R701 T1287 T1285 amod genetic,system
R702 T1288 T1285 punct ", ",system
R703 T1289 T1290 npadvmod neuron,derived
R704 T1290 T1285 amod derived,system
R705 T1291 T1290 punct -,derived
R706 T1292 T1293 compound JNCL,culture
R707 T1293 T1285 compound culture,system
R708 T1294 T1293 compound cell,culture
R709 T1295 T1283 punct ", ",immortalized
R710 T1296 T1283 nsubj we,immortalized
R711 T1297 T1298 compound granule,neurons
R712 T1298 T1283 dobj neurons,immortalized
R713 T1299 T1298 acl cultured,neurons
R714 T1300 T1299 prep from,cultured
R715 T1301 T1302 amod postnatal,day
R716 T1302 T1303 nmod day,cerebella
R717 T1303 T1300 pobj cerebella,from
R718 T1304 T1302 nummod 4,day
R719 T1305 T1302 punct (,day
R720 T1306 T1302 appos P4,day
R721 T1307 T1303 punct ),cerebella
R722 T1308 T1303 prep of,cerebella
R723 T1309 T1310 amod homozygous,mice
R724 T1310 T1308 pobj mice,of
R725 T1311 T1309 cc and,homozygous
R726 T1312 T1309 conj heterozygous,homozygous
R727 T1313 T1310 nmod Cln3Δex7,mice
R728 T1314 T1313 punct /,Cln3Δex7
R729 T1315 T1313 nummod 8,Cln3Δex7
R730 T1316 T1310 amod knock,mice
R731 T1317 T1316 punct -,knock
R732 T1318 T1316 prt in,knock
R733 T1319 T1310 punct ", ",mice
R734 T1320 T1310 cc and,mice
R735 T1321 T1322 amod wild,type
R736 T1322 T1324 compound type,littermates
R737 T1323 T1322 punct -,type
R738 T1324 T1310 conj littermates,mice
R739 T1325 T1283 punct .,immortalized
R740 T1327 T1328 amod Primary,cultures
R741 T1328 T1330 nsubjpass cultures,transduced
R742 T1329 T1328 compound cell,cultures
R743 T1331 T1328 acl enriched,cultures
R744 T1332 T1331 prep for,enriched
R745 T1333 T1334 compound granule,neurons
R746 T1334 T1332 pobj neurons,for
R747 T1335 T1330 auxpass were,transduced
R748 T1336 T1330 prep with,transduced
R749 T1337 T1338 amod retroviral,vector
R750 T1338 T1336 pobj vector,with
R751 T1339 T1338 acl bearing,vector
R752 T1340 T1341 det a,cassette
R753 T1341 T1339 dobj cassette,bearing
R754 T1342 T1341 compound selection,cassette
R755 T1343 T1341 cc and,cassette
R756 T1344 T1345 npadvmod temperature,sensitive
R757 T1345 T1347 amod sensitive,antigen
R758 T1346 T1345 punct -,sensitive
R759 T1347 T1341 conj antigen,cassette
R760 T1348 T1349 nmod tsA58,SV40
R761 T1349 T1347 nmod SV40,antigen
R762 T1350 T1347 amod large,antigen
R763 T1351 T1347 compound T,antigen
R764 T1352 T1330 punct .,transduced
R765 T1354 T1355 nsubj Growth,allowed
R766 T1356 T1354 prep in,Growth
R767 T1357 T1358 npadvmod G418,containing
R768 T1358 T1359 amod containing,medium
R769 T1359 T1356 pobj medium,in
R770 T1360 T1354 prep at,Growth
R771 T1361 T1362 det the,temperature
R772 T1362 T1360 pobj temperature,at
R773 T1363 T1362 amod permissive,temperature
R774 T1364 T1362 punct (,temperature
R775 T1365 T1366 nummod 33,°C
R776 T1366 T1362 appos °C,temperature
R777 T1367 T1355 punct ),allowed
R778 T1368 T1355 prep for,allowed
R779 T1369 T1368 pobj selection,for
R780 T1370 T1369 cc and,selection
R781 T1371 T1369 conj isolation,selection
R782 T1372 T1369 prep of,selection
R783 T1373 T1374 amod multiple,lines
R784 T1374 T1372 pobj lines,of
R785 T1375 T1376 amod clonal,nestin
R786 T1376 T1377 npadvmod nestin,positive
R787 T1377 T1374 amod positive,lines
R788 T1378 T1377 punct -,positive
R789 T1379 T1380 punct (,1a
R790 T1380 T1377 parataxis 1a,positive
R791 T1381 T1380 compound Fig.,1a
R792 T1382 T1380 punct ),1a
R793 T1383 T1377 punct ", ",positive
R794 T1384 T1377 cc and,positive
R795 T1385 T1386 npadvmod GFAP,negative
R796 T1386 T1377 conj negative,positive
R797 T1387 T1386 punct -,negative
R798 T1388 T1389 punct (,1b
R799 T1389 T1386 parataxis 1b,negative
R800 T1390 T1389 compound Fig.,1b
R801 T1391 T1389 punct ),1b
R802 T1392 T1374 punct ", ",lines
R803 T1393 T1374 compound cell,lines
R804 T1394 T1374 prep for,lines
R805 T1395 T1396 det each,genotype
R806 T1396 T1394 pobj genotype,for
R807 T1397 T1355 punct .,allowed
R808 T1399 T1400 det No,differences
R809 T1400 T1403 nsubjpass differences,observed
R810 T1401 T1400 nmod genotype,differences
R811 T1402 T1400 amod specific,differences
R812 T1404 T1403 auxpass were,observed
R813 T1405 T1403 prep in,observed
R814 T1406 T1407 amod cellular,morphology
R815 T1407 T1405 pobj morphology,in
R816 T1408 T1407 cc or,morphology
R817 T1409 T1410 compound doubling,time
R818 T1410 T1407 conj time,morphology
R819 T1411 T1410 punct (,time
R820 T1412 T1413 punct ~,46
R821 T1413 T1414 nummod 46,hours
R822 T1414 T1410 appos hours,time
R823 T1415 T1403 punct ),observed
R824 T1416 T1417 punct (,shown
R825 T1417 T1403 parataxis shown,observed
R826 T1418 T1417 nsubj data,shown
R827 T1419 T1417 neg not,shown
R828 T1420 T1417 punct ),shown
R829 T1421 T1403 punct .,observed
R830 T1423 T1424 mark As,expected
R831 T1424 T1425 advcl expected,lost
R832 T1426 T1425 punct ", ",lost
R833 T1427 T1428 nmod SV40,expression
R834 T1428 T1425 nsubjpass expression,lost
R835 T1429 T1428 amod large,expression
R836 T1430 T1431 compound T,antigen
R837 T1431 T1428 compound antigen,expression
R838 T1432 T1425 auxpass was,lost
R839 T1433 T1425 advmod rapidly,lost
R840 T1434 T1425 cc and,lost
R841 T1435 T1436 compound cell,division
R842 T1436 T1437 nsubj division,ceased
R843 T1437 T1425 conj ceased,lost
R844 T1438 T1439 advmod when,shifted
R845 T1439 T1437 advcl shifted,ceased
R846 T1440 T1439 nsubjpass cells,shifted
R847 T1441 T1439 auxpass were,shifted
R848 T1442 T1439 prep to,shifted
R849 T1443 T1444 det the,temperature
R850 T1444 T1442 pobj temperature,to
R851 T1445 T1444 amod non-permissive,temperature
R852 T1446 T1444 punct (,temperature
R853 T1447 T1448 nummod 39,°C
R854 T1448 T1444 appos °C,temperature
R855 T1449 T1439 punct ),shifted
R856 T1450 T1451 punct (,shown
R857 T1451 T1439 parataxis shown,shifted
R858 T1452 T1451 nsubj data,shown
R859 T1453 T1451 neg not,shown
R860 T1454 T1451 punct ),shown
R861 T1455 T1437 punct .,ceased
R862 T1457 T1458 prep Upon,became
R863 T1459 T1457 pobj addition,Upon
R864 T1460 T1459 prep of,addition
R865 T1461 T1462 amod neuronal,differentiation
R866 T1462 T1463 compound differentiation,cocktail
R867 T1463 T1460 pobj cocktail,of
R868 T1464 T1458 punct ", ",became
R869 T1465 T1466 compound precursor,cells
R870 T1466 T1458 nsubj cells,became
R871 T1467 T1468 npadvmod neuron,like
R872 T1468 T1458 acomp like,became
R873 T1469 T1468 punct -,like
R874 T1470 T1458 prep in,became
R875 T1471 T1470 pobj morphology,in
R876 T1472 T1458 cc and,became
R877 T1473 T1458 conj exhibited,became
R878 T1474 T1475 amod decreased,expression
R879 T1475 T1473 dobj expression,exhibited
R880 T1476 T1475 compound nestin,expression
R881 T1477 T1478 punct (,shown
R882 T1478 T1475 parataxis shown,expression
R883 T1479 T1478 nsubj data,shown
R884 T1480 T1478 neg not,shown
R885 T1481 T1478 punct ),shown
R886 T1482 T1475 cc and,expression
R887 T1483 T1484 amod increased,expression
R888 T1484 T1475 conj expression,expression
R889 T1485 T1484 nmod MAP2,expression
R890 T1486 T1485 cc and,MAP2
R891 T1487 T1485 conj NeuN,MAP2
R892 T1488 T1489 punct (,1d
R893 T1489 T1484 parataxis 1d,expression
R894 T1490 T1489 nmod Fig.,1d
R895 T1491 T1489 nmod 1c,1d
R896 T1492 T1489 punct ",",1d
R897 T1493 T1489 punct ),1d
R898 T1494 T1475 punct ", ",expression
R899 T1495 T1475 cc but,expression
R900 T1496 T1495 neg not,but
R901 T1497 T1475 conj expression,expression
R902 T1498 T1497 prep of,expression
R903 T1499 T1500 det the,marker
R904 T1500 T1498 pobj marker,of
R905 T1501 T1500 compound Purkinje,marker
R906 T1502 T1500 punct ", ",marker
R907 T1503 T1500 appos calbindin,marker
R908 T1504 T1505 punct (,1e
R909 T1505 T1497 parataxis 1e,expression
R910 T1506 T1505 compound Fig.,1e
R911 T1507 T1505 punct ),1e
R912 T1508 T1458 punct .,became