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PMC:539297 / 4164-21937 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T1271 9-19 NN denotes Generation
T1272 20-22 IN denotes of
T1273 23-24 DT denotes a
T1275 25-36 RB denotes genetically
T1276 37-44 JJ denotes precise
T1277 45-55 JJ denotes cerebellar
T1278 56-60 NN denotes JNCL
T1279 61-65 NN denotes cell
T1274 66-71 NN denotes model
T1280 71-304 sentence denotes To generate a precise genetic, neuron-derived JNCL cell culture system, we immortalized granule neurons cultured from postnatal day 4 (P4) cerebella of homozygous and heterozygous Cln3Δex7/8 knock-in mice, and wild-type littermates.
T1281 72-74 TO denotes To
T1282 75-83 VB denotes generate
T1284 84-85 DT denotes a
T1286 86-93 JJ denotes precise
T1287 94-101 JJ denotes genetic
T1288 101-103 , denotes ,
T1289 103-109 NN denotes neuron
T1291 109-110 HYPH denotes -
T1290 110-117 VBN denotes derived
T1292 118-122 NN denotes JNCL
T1294 123-127 NN denotes cell
T1293 128-135 NN denotes culture
T1285 136-142 NN denotes system
T1295 142-144 , denotes ,
T1296 144-146 PRP denotes we
T1283 147-159 VBD denotes immortalized
T1297 160-167 NN denotes granule
T1298 168-175 NNS denotes neurons
T1299 176-184 VBN denotes cultured
T1300 185-189 IN denotes from
T1301 190-199 JJ denotes postnatal
T1302 200-203 NN denotes day
T1304 204-205 CD denotes 4
T1305 206-207 -LRB- denotes (
T1306 207-209 NN denotes P4
T1307 209-210 -RRB- denotes )
T1303 211-220 NNS denotes cerebella
T1308 221-223 IN denotes of
T1309 224-234 JJ denotes homozygous
T1311 235-238 CC denotes and
T1312 239-251 JJ denotes heterozygous
T1313 252-260 NN denotes Cln3Δex7
T1314 260-261 HYPH denotes /
T1315 261-262 CD denotes 8
T1316 263-268 VB denotes knock
T1317 268-269 HYPH denotes -
T1318 269-271 RP denotes in
T1310 272-276 NNS denotes mice
T1319 276-278 , denotes ,
T1320 278-281 CC denotes and
T1321 282-286 JJ denotes wild
T1323 286-287 HYPH denotes -
T1322 287-291 NN denotes type
T1324 292-303 NNS denotes littermates
T1325 303-304 . denotes .
T1326 304-477 sentence denotes Primary cell cultures enriched for granule neurons were transduced with retroviral vector bearing a selection cassette and temperature-sensitive tsA58 SV40 large T antigen.
T1327 305-312 JJ denotes Primary
T1329 313-317 NN denotes cell
T1328 318-326 NNS denotes cultures
T1331 327-335 VBN denotes enriched
T1332 336-339 IN denotes for
T1333 340-347 NN denotes granule
T1334 348-355 NNS denotes neurons
T1335 356-360 VBD denotes were
T1330 361-371 VBN denotes transduced
T1336 372-376 IN denotes with
T1337 377-387 JJ denotes retroviral
T1338 388-394 NN denotes vector
T1339 395-402 VBG denotes bearing
T1340 403-404 DT denotes a
T1342 405-414 NN denotes selection
T1341 415-423 NN denotes cassette
T1343 424-427 CC denotes and
T1344 428-439 NN denotes temperature
T1346 439-440 HYPH denotes -
T1345 440-449 JJ denotes sensitive
T1348 450-455 NN denotes tsA58
T1349 456-460 NN denotes SV40
T1350 461-466 JJ denotes large
T1351 467-468 NN denotes T
T1347 469-476 NN denotes antigen
T1352 476-477 . denotes .
T1353 477-688 sentence denotes Growth in G418 containing medium at the permissive temperature (33°C) allowed for selection and isolation of multiple clonal nestin-positive (Fig. 1a), and GFAP-negative (Fig. 1b), cell lines for each genotype.
T1354 478-484 NN denotes Growth
T1356 485-487 IN denotes in
T1357 488-492 NN denotes G418
T1358 493-503 VBG denotes containing
T1359 504-510 NN denotes medium
T1360 511-513 IN denotes at
T1361 514-517 DT denotes the
T1363 518-528 JJ denotes permissive
T1362 529-540 NN denotes temperature
T1364 541-542 -LRB- denotes (
T1365 542-544 CD denotes 33
T1366 544-546 NN denotes °C
T1367 546-547 -RRB- denotes )
T1355 548-555 VBN denotes allowed
T1368 556-559 IN denotes for
T1369 560-569 NN denotes selection
T1370 570-573 CC denotes and
T1371 574-583 NN denotes isolation
T1372 584-586 IN denotes of
T1373 587-595 JJ denotes multiple
T1375 596-602 JJ denotes clonal
T1376 603-609 NN denotes nestin
T1378 609-610 HYPH denotes -
T1377 610-618 JJ denotes positive
T1379 619-620 -LRB- denotes (
T1381 620-624 NN denotes Fig.
T1380 625-627 NN denotes 1a
T1382 627-628 -RRB- denotes )
T1383 628-630 , denotes ,
T1384 630-633 CC denotes and
T1385 634-638 NN denotes GFAP
T1387 638-639 HYPH denotes -
T1386 639-647 JJ denotes negative
T1388 648-649 -LRB- denotes (
T1390 649-653 NN denotes Fig.
T1389 654-656 NN denotes 1b
T1391 656-657 -RRB- denotes )
T1392 657-659 , denotes ,
T1393 659-663 NN denotes cell
T1374 664-669 NNS denotes lines
T1394 670-673 IN denotes for
T1395 674-678 DT denotes each
T1396 679-687 NN denotes genotype
T1397 687-688 . denotes .
T1398 688-805 sentence denotes No genotype specific differences were observed in cellular morphology or doubling time (~46 hours) (data not shown).
T1399 689-691 DT denotes No
T1401 692-700 NN denotes genotype
T1402 701-709 JJ denotes specific
T1400 710-721 NNS denotes differences
T1404 722-726 VBD denotes were
T1403 727-735 VBN denotes observed
T1405 736-738 IN denotes in
T1406 739-747 JJ denotes cellular
T1407 748-758 NN denotes morphology
T1408 759-761 CC denotes or
T1409 762-770 NN denotes doubling
T1410 771-775 NN denotes time
T1411 776-777 -LRB- denotes (
T1412 777-778 SYM denotes ~
T1413 778-780 CD denotes 46
T1414 781-786 NNS denotes hours
T1415 786-787 -RRB- denotes )
T1416 788-789 -LRB- denotes (
T1418 789-793 NNS denotes data
T1419 794-797 RB denotes not
T1417 798-803 VBN denotes shown
T1420 803-804 -RRB- denotes )
T1421 804-805 . denotes .
T1422 805-975 sentence denotes As expected, SV40 large T antigen expression was rapidly lost and cell division ceased when cells were shifted to the non-permissive temperature (39°C) (data not shown).
T1423 806-808 IN denotes As
T1424 809-817 VBN denotes expected
T1426 817-819 , denotes ,
T1427 819-823 NN denotes SV40
T1429 824-829 JJ denotes large
T1430 830-831 NN denotes T
T1431 832-839 NN denotes antigen
T1428 840-850 NN denotes expression
T1432 851-854 VBD denotes was
T1433 855-862 RB denotes rapidly
T1425 863-867 VBN denotes lost
T1434 868-871 CC denotes and
T1435 872-876 NN denotes cell
T1436 877-885 NN denotes division
T1437 886-892 VBD denotes ceased
T1438 893-897 WRB denotes when
T1440 898-903 NNS denotes cells
T1441 904-908 VBD denotes were
T1439 909-916 VBN denotes shifted
T1442 917-919 IN denotes to
T1443 920-923 DT denotes the
T1445 924-938 JJ denotes non-permissive
T1444 939-950 NN denotes temperature
T1446 951-952 -LRB- denotes (
T1447 952-954 CD denotes 39
T1448 954-956 NN denotes °C
T1449 956-957 -RRB- denotes )
T1450 958-959 -LRB- denotes (
T1452 959-963 NNS denotes data
T1453 964-967 RB denotes not
T1451 968-973 VBN denotes shown
T1454 973-974 -RRB- denotes )
T1455 974-975 . denotes .
T1456 975-1252 sentence denotes Upon addition of neuronal differentiation cocktail, precursor cells became neuron-like in morphology and exhibited decreased nestin expression (data not shown) and increased MAP2 and NeuN expression (Fig. 1c,1d), but not expression of the Purkinje marker, calbindin (Fig. 1e).
T1457 976-980 IN denotes Upon
T1459 981-989 NN denotes addition
T1460 990-992 IN denotes of
T1461 993-1001 JJ denotes neuronal
T1462 1002-1017 NN denotes differentiation
T1463 1018-1026 NN denotes cocktail
T1464 1026-1028 , denotes ,
T1465 1028-1037 NN denotes precursor
T1466 1038-1043 NNS denotes cells
T1458 1044-1050 VBD denotes became
T1467 1051-1057 NN denotes neuron
T1469 1057-1058 HYPH denotes -
T1468 1058-1062 JJ denotes like
T1470 1063-1065 IN denotes in
T1471 1066-1076 NN denotes morphology
T1472 1077-1080 CC denotes and
T1473 1081-1090 VBD denotes exhibited
T1474 1091-1100 VBN denotes decreased
T1476 1101-1107 NN denotes nestin
T1475 1108-1118 NN denotes expression
T1477 1119-1120 -LRB- denotes (
T1479 1120-1124 NNS denotes data
T1480 1125-1128 RB denotes not
T1478 1129-1134 VBN denotes shown
T1481 1134-1135 -RRB- denotes )
T1482 1136-1139 CC denotes and
T1483 1140-1149 VBN denotes increased
T1485 1150-1154 NN denotes MAP2
T1486 1155-1158 CC denotes and
T1487 1159-1163 NN denotes NeuN
T1484 1164-1174 NN denotes expression
T1488 1175-1176 -LRB- denotes (
T1490 1176-1180 NN denotes Fig.
T1491 1181-1183 NN denotes 1c
T1492 1183-1184 , denotes ,
T1489 1184-1186 NN denotes 1d
T1493 1186-1187 -RRB- denotes )
T1494 1187-1189 , denotes ,
T1495 1189-1192 CC denotes but
T1496 1193-1196 RB denotes not
T1497 1197-1207 NN denotes expression
T1498 1208-1210 IN denotes of
T1499 1211-1214 DT denotes the
T1501 1215-1223 NNP denotes Purkinje
T1500 1224-1230 NN denotes marker
T1502 1230-1232 , denotes ,
T1503 1232-1241 NN denotes calbindin
T1504 1242-1243 -LRB- denotes (
T1506 1243-1247 NN denotes Fig.
T1505 1248-1250 NN denotes 1e
T1507 1250-1251 -RRB- denotes )
T1508 1251-1252 . denotes .
T7982 1263-1271 JJ denotes Neuronal
T7983 1272-1278 NN denotes marker
T7984 1279-1289 NN denotes expression
T7986 1290-1292 IN denotes in
T7987 1293-1299 NN denotes CbCln3
T7989 1299-1300 SYM denotes +
T7990 1300-1301 HYPH denotes /
T7991 1301-1302 SYM denotes +
T7988 1303-1308 NNS denotes cells
T7992 1309-1325 NN denotes Characterization
T7993 1326-1328 IN denotes of
T7994 1329-1335 NN denotes CbCln3
T7996 1335-1336 SYM denotes +
T7997 1336-1337 HYPH denotes /
T7998 1337-1338 SYM denotes +
T7995 1339-1344 NNS denotes cells
T7999 1345-1347 IN denotes by
T8000 1348-1366 NN denotes immunofluorescence
T8001 1367-1371 IN denotes with
T8002 1372-1378 NN denotes marker
T8003 1379-1389 NNS denotes antibodies
T8004 1390-1392 VBZ denotes is
T7985 1393-1398 VBN denotes shown
T8005 1398-1399 . denotes .
T8006 1399-1526 sentence denotes CbCln3+/+ precursors exhibit nestin expression (a) but not GFAP expression (b), consistent with a neuronal precursor identity.
T8007 1400-1406 NN denotes CbCln3
T8009 1406-1407 SYM denotes +
T8010 1407-1408 HYPH denotes /
T8011 1408-1409 SYM denotes +
T8008 1410-1420 NNS denotes precursors
T8012 1421-1428 VBP denotes exhibit
T8013 1429-1435 NN denotes nestin
T8014 1436-1446 NN denotes expression
T8015 1447-1448 -LRB- denotes (
T8016 1448-1449 NN denotes a
T8017 1449-1450 -RRB- denotes )
T8018 1451-1454 CC denotes but
T8019 1455-1458 RB denotes not
T8020 1459-1463 NN denotes GFAP
T8021 1464-1474 NN denotes expression
T8022 1475-1476 -LRB- denotes (
T8023 1476-1477 NN denotes b
T8024 1477-1478 -RRB- denotes )
T8025 1478-1480 , denotes ,
T8026 1480-1490 JJ denotes consistent
T8027 1491-1495 IN denotes with
T8028 1496-1497 DT denotes a
T8030 1498-1506 JJ denotes neuronal
T8031 1507-1516 NN denotes precursor
T8029 1517-1525 NN denotes identity
T8032 1525-1526 . denotes .
T8033 1526-1744 sentence denotes Upon stimulation with a differentiation cocktail (see Methods), CbCln3+/+ cells achieved neuron-like morphology, with rounded cell bodies and extension of processes, and MAP2 (c) and NeuN (d) expression was increased.
T8034 1527-1531 IN denotes Upon
T8036 1532-1543 NN denotes stimulation
T8037 1544-1548 IN denotes with
T8038 1549-1550 DT denotes a
T8040 1551-1566 NN denotes differentiation
T8039 1567-1575 NN denotes cocktail
T8041 1576-1577 -LRB- denotes (
T8042 1577-1580 VB denotes see
T8043 1581-1588 NNS denotes Methods
T8044 1588-1589 -RRB- denotes )
T8045 1589-1591 , denotes ,
T8046 1591-1597 NN denotes CbCln3
T8048 1597-1598 SYM denotes +
T8049 1598-1599 HYPH denotes /
T8050 1599-1600 SYM denotes +
T8047 1601-1606 NNS denotes cells
T8035 1607-1615 VBD denotes achieved
T8051 1616-1622 NN denotes neuron
T8053 1622-1623 HYPH denotes -
T8052 1623-1627 JJ denotes like
T8054 1628-1638 NN denotes morphology
T8055 1638-1640 , denotes ,
T8056 1640-1644 IN denotes with
T8057 1645-1652 JJ denotes rounded
T8059 1653-1657 NN denotes cell
T8058 1658-1664 NNS denotes bodies
T8060 1665-1668 CC denotes and
T8061 1669-1678 NN denotes extension
T8062 1679-1681 IN denotes of
T8063 1682-1691 NNS denotes processes
T8064 1691-1693 , denotes ,
T8065 1693-1696 CC denotes and
T8066 1697-1701 NN denotes MAP2
T8068 1702-1703 -LRB- denotes (
T8069 1703-1704 NN denotes c
T8070 1704-1705 -RRB- denotes )
T8071 1706-1709 CC denotes and
T8072 1710-1714 NN denotes NeuN
T8073 1715-1716 -LRB- denotes (
T8074 1716-1717 NN denotes d
T8075 1717-1718 -RRB- denotes )
T8067 1719-1729 NN denotes expression
T8077 1730-1733 VBD denotes was
T8076 1734-1743 VBN denotes increased
T8078 1743-1744 . denotes .
T8079 1744-1819 sentence denotes CbCln3+/+ cells are negative for the Purkinje neuron marker calbindin (e).
T8080 1745-1751 NN denotes CbCln3
T8082 1751-1752 SYM denotes +
T8083 1752-1753 HYPH denotes /
T8084 1753-1754 SYM denotes +
T8081 1755-1760 NNS denotes cells
T8085 1761-1764 VBP denotes are
T8086 1765-1773 JJ denotes negative
T8087 1774-1777 IN denotes for
T8088 1778-1781 DT denotes the
T8090 1782-1790 NNP denotes Purkinje
T8091 1791-1797 NN denotes neuron
T8089 1798-1804 NN denotes marker
T8092 1805-1814 NN denotes calbindin
T8093 1815-1816 -LRB- denotes (
T8094 1816-1817 NN denotes e
T8095 1817-1818 -RRB- denotes )
T8096 1818-1819 . denotes .
T8097 1819-1924 sentence denotes CbCln3+/Δex7/8 and CbCln3Δex7/8/Δex7/8 cell lines exhibited identical marker immunofluorescence results.
T8098 1820-1826 NN denotes CbCln3
T8100 1826-1827 SYM denotes +
T8101 1827-1828 HYPH denotes /
T8099 1828-1832 NN denotes Δex7
T8103 1832-1833 HYPH denotes /
T8104 1833-1834 CD denotes 8
T8105 1835-1838 CC denotes and
T8106 1839-1849 NN denotes CbCln3Δex7
T8108 1849-1850 HYPH denotes /
T8109 1850-1851 CD denotes 8
T8110 1851-1852 HYPH denotes /
T8107 1852-1856 NN denotes Δex7
T8111 1856-1857 HYPH denotes /
T8112 1857-1858 CD denotes 8
T8113 1859-1863 NN denotes cell
T8102 1864-1869 NNS denotes lines
T8114 1870-1879 VBD denotes exhibited
T8115 1880-1889 JJ denotes identical
T8117 1890-1896 NN denotes marker
T8118 1897-1915 NN denotes immunofluorescence
T8116 1916-1923 NNS denotes results
T8119 1923-1924 . denotes .
T8120 1924-1979 sentence denotes a, b) 20 × magnification; c, d, e) 40 × magnification.
T8121 1925-1926 LS denotes a
T8123 1926-1928 , denotes ,
T8124 1928-1929 LS denotes b
T8125 1929-1930 -RRB- denotes )
T8126 1931-1933 CD denotes 20
T8127 1934-1935 SYM denotes ×
T8122 1936-1949 NN denotes magnification
T8128 1949-1950 : denotes ;
T8129 1951-1952 LS denotes c
T8131 1952-1954 , denotes ,
T8132 1954-1955 LS denotes d
T8133 1955-1957 , denotes ,
T8134 1957-1958 LS denotes e
T8135 1958-1959 -RRB- denotes )
T8136 1960-1962 CD denotes 40
T8137 1963-1964 SYM denotes ×
T8130 1965-1978 NN denotes magnification
T8138 1978-1979 . denotes .
T1775 1981-1991 JJ denotes Homozygous
T1777 1992-2002 NN denotes CbCln3Δex7
T1778 2002-2003 HYPH denotes /
T1779 2003-2004 CD denotes 8
T1776 2005-2010 NNS denotes cells
T1780 2011-2018 VBP denotes express
T1781 2019-2025 NN denotes mutant
T1782 2026-2034 NN denotes battenin
T1783 2035-2038 CC denotes and
T1784 2039-2046 VBP denotes display
T1785 2047-2051 NN denotes JNCL
T1787 2051-2052 HYPH denotes -
T1786 2052-2056 JJ denotes like
T1788 2057-2066 NN denotes pathology
T1789 2066-2148 sentence denotes Homozygous Cb Cln3Δex7/8 cells were first examined for JNCL-like characteristics.
T1790 2067-2077 JJ denotes Homozygous
T1792 2078-2080 NN denotes Cb
T1793 2081-2089 NN denotes Cln3Δex7
T1794 2089-2090 HYPH denotes /
T1795 2090-2091 CD denotes 8
T1791 2092-2097 NNS denotes cells
T1797 2098-2102 VBD denotes were
T1798 2103-2108 RB denotes first
T1796 2109-2117 VBN denotes examined
T1799 2118-2121 IN denotes for
T1800 2122-2126 NN denotes JNCL
T1802 2126-2127 HYPH denotes -
T1801 2127-2131 JJ denotes like
T1803 2132-2147 NNS denotes characteristics
T1804 2147-2148 . denotes .
T1805 2148-2330 sentence denotes Homozygous Cln3Δex7/8 knock-in mice express multiple Cln3 mRNA splice variants and mutant battenin protein that is detectable by batp1 antibody recognizing C-terminal epitopes [12].
T1806 2149-2159 JJ denotes Homozygous
T1808 2160-2168 NN denotes Cln3Δex7
T1809 2168-2169 HYPH denotes /
T1810 2169-2170 CD denotes 8
T1811 2171-2176 VB denotes knock
T1812 2176-2177 HYPH denotes -
T1813 2177-2179 RP denotes in
T1807 2180-2184 NNS denotes mice
T1814 2185-2192 VBP denotes express
T1815 2193-2201 JJ denotes multiple
T1817 2202-2206 NN denotes Cln3
T1819 2207-2211 NN denotes mRNA
T1818 2212-2218 NN denotes splice
T1816 2219-2227 NNS denotes variants
T1820 2228-2231 CC denotes and
T1821 2232-2238 NN denotes mutant
T1823 2239-2247 NN denotes battenin
T1822 2248-2255 NN denotes protein
T1824 2256-2260 WDT denotes that
T1825 2261-2263 VBZ denotes is
T1826 2264-2274 JJ denotes detectable
T1827 2275-2277 IN denotes by
T1828 2278-2283 NN denotes batp1
T1829 2284-2292 NN denotes antibody
T1830 2293-2304 VBG denotes recognizing
T1832 2305-2306 NN denotes C
T1834 2306-2307 HYPH denotes -
T1833 2307-2315 JJ denotes terminal
T1831 2316-2324 NNS denotes epitopes
T1835 2325-2326 -LRB- denotes [
T1836 2326-2328 CD denotes 12
T1837 2328-2329 -RRB- denotes ]
T1838 2329-2330 . denotes .
T1839 2330-2452 sentence denotes To assess this molecular phenotype in CbCln3Δex7/8 cells, RT-PCR and anti-battenin (batp1) immunostaining were performed.
T1840 2331-2333 TO denotes To
T1841 2334-2340 VB denotes assess
T1843 2341-2345 DT denotes this
T1845 2346-2355 JJ denotes molecular
T1844 2356-2365 NN denotes phenotype
T1846 2366-2368 IN denotes in
T1847 2369-2379 NN denotes CbCln3Δex7
T1849 2379-2380 HYPH denotes /
T1850 2380-2381 CD denotes 8
T1848 2382-2387 NNS denotes cells
T1851 2387-2389 , denotes ,
T1852 2389-2391 NN denotes RT
T1854 2391-2392 HYPH denotes -
T1853 2392-2395 NN denotes PCR
T1855 2396-2399 CC denotes and
T1856 2400-2413 JJ denotes anti-battenin
T1858 2414-2415 -LRB- denotes (
T1859 2415-2420 NN denotes batp1
T1860 2420-2421 -RRB- denotes )
T1857 2422-2436 NN denotes immunostaining
T1861 2437-2441 VBD denotes were
T1842 2442-2451 VBN denotes performed
T1862 2451-2452 . denotes .
T1863 2452-2659 sentence denotes As shown in Figure 2, Cln3 mRNA isoforms in wild-type and homozygous cells were similar to those observed in total RNA isolated from wild-type or homozygous Cln3Δex7/8 knock-in brain, respectively (Fig. 2).
T1864 2453-2455 IN denotes As
T1865 2456-2461 VBN denotes shown
T1867 2462-2464 IN denotes in
T1868 2465-2471 NN denotes Figure
T1869 2472-2473 CD denotes 2
T1870 2473-2475 , denotes ,
T1871 2475-2479 NN denotes Cln3
T1873 2480-2484 NN denotes mRNA
T1872 2485-2493 NNS denotes isoforms
T1874 2494-2496 IN denotes in
T1875 2497-2501 JJ denotes wild
T1877 2501-2502 HYPH denotes -
T1876 2502-2506 NN denotes type
T1879 2507-2510 CC denotes and
T1880 2511-2521 JJ denotes homozygous
T1878 2522-2527 NNS denotes cells
T1866 2528-2532 VBD denotes were
T1881 2533-2540 JJ denotes similar
T1882 2541-2543 IN denotes to
T1883 2544-2549 DT denotes those
T1884 2550-2558 VBN denotes observed
T1885 2559-2561 IN denotes in
T1886 2562-2567 JJ denotes total
T1887 2568-2571 NN denotes RNA
T1888 2572-2580 VBN denotes isolated
T1889 2581-2585 IN denotes from
T1890 2586-2590 JJ denotes wild
T1892 2590-2591 HYPH denotes -
T1891 2591-2595 NN denotes type
T1894 2596-2598 CC denotes or
T1895 2599-2609 JJ denotes homozygous
T1896 2610-2618 NN denotes Cln3Δex7
T1897 2618-2619 HYPH denotes /
T1898 2619-2620 CD denotes 8
T1899 2621-2626 VB denotes knock
T1900 2626-2627 HYPH denotes -
T1901 2627-2629 RP denotes in
T1893 2630-2635 NN denotes brain
T1902 2635-2637 , denotes ,
T1903 2637-2649 RB denotes respectively
T1904 2650-2651 -LRB- denotes (
T1905 2651-2655 NN denotes Fig.
T1906 2656-2657 CD denotes 2
T1907 2657-2658 -RRB- denotes )
T1908 2658-2659 . denotes .
T1909 2659-2861 sentence denotes In addition, batp1 immunostaining detected mutant battenin product in homozygous CbCln3Δex7/8 cells, in a similar albeit reduced cytoplasmic, vesicular staining pattern as that seen in wild-type cells.
T1910 2660-2662 IN denotes In
T1912 2663-2671 NN denotes addition
T1913 2671-2673 , denotes ,
T1914 2673-2678 NN denotes batp1
T1915 2679-2693 NN denotes immunostaining
T1911 2694-2702 VBD denotes detected
T1916 2703-2709 NN denotes mutant
T1918 2710-2718 NN denotes battenin
T1917 2719-2726 NN denotes product
T1919 2727-2729 IN denotes in
T1920 2730-2740 JJ denotes homozygous
T1922 2741-2751 NN denotes CbCln3Δex7
T1923 2751-2752 HYPH denotes /
T1924 2752-2753 CD denotes 8
T1921 2754-2759 NNS denotes cells
T1925 2759-2761 , denotes ,
T1926 2761-2763 IN denotes in
T1927 2764-2765 DT denotes a
T1929 2766-2773 JJ denotes similar
T1930 2774-2780 IN denotes albeit
T1931 2781-2788 VBN denotes reduced
T1932 2789-2800 JJ denotes cytoplasmic
T1933 2800-2802 , denotes ,
T1934 2802-2811 JJ denotes vesicular
T1935 2812-2820 NN denotes staining
T1928 2821-2828 NN denotes pattern
T1936 2829-2831 IN denotes as
T1937 2832-2836 DT denotes that
T1938 2837-2841 VBN denotes seen
T1939 2842-2844 IN denotes in
T1940 2845-2849 JJ denotes wild
T1942 2849-2850 HYPH denotes -
T1941 2850-2854 NN denotes type
T1943 2855-2860 NNS denotes cells
T1944 2860-2861 . denotes .
T1945 2861-3047 sentence denotes Batp1 signal exhibited some overlap with the lysosomal marker, Lamp1, but had more significant overlap with early endosome antigen 1 (EEA1) and the late endosomal marker, Rab7 (Fig. 3).
T1946 2862-2867 NN denotes Batp1
T1947 2868-2874 NN denotes signal
T1948 2875-2884 VBD denotes exhibited
T1949 2885-2889 DT denotes some
T1950 2890-2897 NN denotes overlap
T1951 2898-2902 IN denotes with
T1952 2903-2906 DT denotes the
T1954 2907-2916 JJ denotes lysosomal
T1953 2917-2923 NN denotes marker
T1955 2923-2925 , denotes ,
T1956 2925-2930 NN denotes Lamp1
T1957 2930-2932 , denotes ,
T1958 2932-2935 CC denotes but
T1959 2936-2939 VBD denotes had
T1960 2940-2944 RBR denotes more
T1961 2945-2956 JJ denotes significant
T1962 2957-2964 NN denotes overlap
T1963 2965-2969 IN denotes with
T1964 2970-2975 JJ denotes early
T1966 2976-2984 NN denotes endosome
T1965 2985-2992 NN denotes antigen
T1967 2993-2994 CD denotes 1
T1968 2995-2996 -LRB- denotes (
T1969 2996-3000 NN denotes EEA1
T1970 3000-3001 -RRB- denotes )
T1971 3002-3005 CC denotes and
T1972 3006-3009 DT denotes the
T1974 3010-3014 JJ denotes late
T1975 3015-3024 JJ denotes endosomal
T1973 3025-3031 NN denotes marker
T1976 3031-3033 , denotes ,
T1977 3033-3037 NN denotes Rab7
T1978 3038-3039 -LRB- denotes (
T1979 3039-3043 NN denotes Fig.
T1980 3044-3045 CD denotes 3
T1981 3045-3046 -RRB- denotes )
T1982 3046-3047 . denotes .
T1983 3047-3175 sentence denotes Only limited overlap was observed with recycling endosomes, as determined by transferrin receptor co-staining (data not shown).
T1984 3048-3052 RB denotes Only
T1986 3053-3060 VBN denotes limited
T1985 3061-3068 NN denotes overlap
T1988 3069-3072 VBD denotes was
T1987 3073-3081 VBN denotes observed
T1989 3082-3086 IN denotes with
T1990 3087-3096 VBG denotes recycling
T1991 3097-3106 NNS denotes endosomes
T1992 3106-3108 , denotes ,
T1993 3108-3110 IN denotes as
T1994 3111-3121 VBN denotes determined
T1995 3122-3124 IN denotes by
T1996 3125-3136 NN denotes transferrin
T1997 3137-3145 NN denotes receptor
T1998 3146-3157 NN denotes co-staining
T1999 3158-3159 -LRB- denotes (
T2001 3159-3163 NNS denotes data
T2002 3164-3167 RB denotes not
T2000 3168-3173 VBN denotes shown
T2003 3173-3174 -RRB- denotes )
T2004 3174-3175 . denotes .
T2005 3175-3430 sentence denotes Intriguingly, Lamp1 and EEA1 immunocytochemical distribution were altered in homozygous CbCln3Δex7/8 cells, with less perinuclear clustering than in wild-type cells, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells (Fig. 3).
T2006 3176-3188 RB denotes Intriguingly
T2008 3188-3190 , denotes ,
T2009 3190-3195 NN denotes Lamp1
T2011 3196-3199 CC denotes and
T2012 3200-3204 NN denotes EEA1
T2013 3205-3223 JJ denotes immunocytochemical
T2010 3224-3236 NN denotes distribution
T2014 3237-3241 VBD denotes were
T2007 3242-3249 VBN denotes altered
T2015 3250-3252 IN denotes in
T2016 3253-3263 JJ denotes homozygous
T2018 3264-3274 NN denotes CbCln3Δex7
T2019 3274-3275 HYPH denotes /
T2020 3275-3276 CD denotes 8
T2017 3277-3282 NNS denotes cells
T2021 3282-3284 , denotes ,
T2022 3284-3288 IN denotes with
T2023 3289-3293 JJR denotes less
T2025 3294-3305 JJ denotes perinuclear
T2024 3306-3316 NN denotes clustering
T2026 3317-3321 IN denotes than
T2027 3322-3324 IN denotes in
T2028 3325-3329 JJ denotes wild
T2030 3329-3330 HYPH denotes -
T2029 3330-3334 NN denotes type
T2031 3335-3340 NNS denotes cells
T2032 3340-3342 , denotes ,
T2033 3342-3345 CC denotes and
T2034 3346-3350 NN denotes Rab7
T2035 3351-3359 NN denotes staining
T2036 3360-3363 VBD denotes was
T2037 3364-3374 RB denotes frequently
T2038 3375-3379 RBR denotes less
T2039 3380-3387 JJ denotes intense
T2040 3388-3390 IN denotes in
T2041 3391-3401 JJ denotes homozygous
T2043 3402-3412 NN denotes CbCln3Δex7
T2044 3412-3413 HYPH denotes /
T2045 3413-3414 CD denotes 8
T2042 3415-3420 NNS denotes cells
T2046 3421-3422 -LRB- denotes (
T2047 3422-3426 NN denotes Fig.
T2048 3427-3428 CD denotes 3
T2049 3428-3429 -RRB- denotes )
T2050 3429-3430 . denotes .
T2051 3430-3637 sentence denotes Heterozygous CbCln3Δex7/8 cells contained a mixture of Cln3 mRNA products from both the wild-type allele and the mutant allele, and batp1 signal was similar to that seen in wild-type cells (data not shown).
T2052 3431-3443 JJ denotes Heterozygous
T2054 3444-3454 NN denotes CbCln3Δex7
T2055 3454-3455 HYPH denotes /
T2056 3455-3456 CD denotes 8
T2053 3457-3462 NNS denotes cells
T2057 3463-3472 VBD denotes contained
T2058 3473-3474 DT denotes a
T2059 3475-3482 NN denotes mixture
T2060 3483-3485 IN denotes of
T2061 3486-3490 NN denotes Cln3
T2063 3491-3495 NN denotes mRNA
T2062 3496-3504 NNS denotes products
T2064 3505-3509 IN denotes from
T2065 3510-3514 CC denotes both
T2067 3515-3518 DT denotes the
T2068 3519-3523 JJ denotes wild
T2070 3523-3524 HYPH denotes -
T2069 3524-3528 NN denotes type
T2066 3529-3535 NN denotes allele
T2071 3536-3539 CC denotes and
T2072 3540-3543 DT denotes the
T2074 3544-3550 NN denotes mutant
T2073 3551-3557 NN denotes allele
T2075 3557-3559 , denotes ,
T2076 3559-3562 CC denotes and
T2077 3563-3568 NN denotes batp1
T2078 3569-3575 NN denotes signal
T2079 3576-3579 VBD denotes was
T2080 3580-3587 JJ denotes similar
T2081 3588-3590 IN denotes to
T2082 3591-3595 DT denotes that
T2083 3596-3600 VBN denotes seen
T2084 3601-3603 IN denotes in
T2085 3604-3608 JJ denotes wild
T2087 3608-3609 HYPH denotes -
T2086 3609-3613 NN denotes type
T2088 3614-3619 NNS denotes cells
T2089 3620-3621 -LRB- denotes (
T2091 3621-3625 NNS denotes data
T2092 3626-3629 RB denotes not
T2090 3630-3635 VBN denotes shown
T2093 3635-3636 -RRB- denotes )
T2094 3636-3637 . denotes .
T2095 3637-5430 sentence denotes Figure 2 RT-PCR of Cln3 mRNA in wild-type and homozygous CbCln3Δex7/8 cells Cln3 Exon1-forward, Exon 15-reverse RT-PCR products are shown, from total wild-type (+/+) or homozygous mutant (Δex7/8/Δex7/8) brain and cell line RNA. Brain and cell line RT-PCR reaction products had identical band patterns on ethidium-bromide stained agarose gels. Wild-type RT-PCR product was a single ~1.6 kb band and mutant products were ~1.6, ~1.5, ~1.4, ~1.35, and ~1.3 kb, representing multiple mutant splice variants. Figure 3 Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. During sub-confluent growth conditions, neither wild-type nor homozygous CbCln3Δex7/8 cells displayed autofluorescence or subunit c inclusion formation (data not shown).
T8215 3648-3650 NN denotes RT
T8217 3650-3651 HYPH denotes -
T8216 3651-3654 NN denotes PCR
T8219 3655-3657 IN denotes of
T8220 3658-3662 NN denotes Cln3
T8221 3663-3667 NN denotes mRNA
T8222 3668-3670 IN denotes in
T8223 3671-3675 JJ denotes wild
T8225 3675-3676 HYPH denotes -
T8224 3676-3680 NN denotes type
T8227 3681-3684 CC denotes and
T8228 3685-3695 JJ denotes homozygous
T8229 3696-3706 NN denotes CbCln3Δex7
T8230 3706-3707 HYPH denotes /
T8231 3707-3708 CD denotes 8
T8226 3709-3714 NNS denotes cells
T8232 3715-3719 NN denotes Cln3
T8234 3720-3725 NN denotes Exon1
T8236 3725-3726 HYPH denotes -
T8235 3726-3733 JJ denotes forward
T8238 3733-3735 , denotes ,
T8239 3735-3739 NN denotes Exon
T8240 3740-3742 CD denotes 15
T8241 3742-3743 HYPH denotes -
T8237 3743-3750 JJ denotes reverse
T8242 3751-3753 NN denotes RT
T8244 3753-3754 HYPH denotes -
T8243 3754-3757 NN denotes PCR
T8233 3758-3766 NNS denotes products
T8245 3767-3770 VBP denotes are
T8218 3771-3776 VBN denotes shown
T8246 3776-3778 , denotes ,
T8247 3778-3782 IN denotes from
T8248 3783-3788 JJ denotes total
T8250 3789-3793 JJ denotes wild
T8252 3793-3794 HYPH denotes -
T8251 3794-3798 NN denotes type
T8253 3799-3800 -LRB- denotes (
T8254 3800-3801 SYM denotes +
T8256 3801-3802 HYPH denotes /
T8255 3802-3803 SYM denotes +
T8257 3803-3804 -RRB- denotes )
T8258 3805-3807 CC denotes or
T8259 3808-3818 JJ denotes homozygous
T8260 3819-3825 NN denotes mutant
T8261 3826-3827 -LRB- denotes (
T8262 3827-3831 NN denotes Δex7
T8263 3831-3832 HYPH denotes /
T8264 3832-3833 CD denotes 8
T8265 3833-3834 HYPH denotes /
T8266 3834-3838 NN denotes Δex7
T8267 3838-3839 HYPH denotes /
T8268 3839-3840 CD denotes 8
T8269 3840-3841 -RRB- denotes )
T8270 3842-3847 NN denotes brain
T8271 3848-3851 CC denotes and
T8272 3852-3856 NN denotes cell
T8273 3857-3861 NN denotes line
T8249 3862-3865 NN denotes RNA
T8274 3865-3866 . denotes .
T8275 3866-3981 sentence denotes Brain and cell line RT-PCR reaction products had identical band patterns on ethidium-bromide stained agarose gels.
T8276 3867-3872 NN denotes Brain
T8278 3873-3876 CC denotes and
T8279 3877-3881 NN denotes cell
T8280 3882-3886 NN denotes line
T8281 3887-3889 NN denotes RT
T8283 3889-3890 HYPH denotes -
T8282 3890-3893 NN denotes PCR
T8284 3894-3902 NN denotes reaction
T8277 3903-3911 NNS denotes products
T8285 3912-3915 VBD denotes had
T8286 3916-3925 JJ denotes identical
T8288 3926-3930 NN denotes band
T8287 3931-3939 NNS denotes patterns
T8289 3940-3942 IN denotes on
T8290 3943-3951 NN denotes ethidium
T8292 3951-3952 HYPH denotes -
T8291 3952-3959 NN denotes bromide
T8293 3960-3967 VBN denotes stained
T8295 3968-3975 NN denotes agarose
T8294 3976-3980 NNS denotes gels
T8296 3980-3981 . denotes .
T8297 3981-4141 sentence denotes Wild-type RT-PCR product was a single ~1.6 kb band and mutant products were ~1.6, ~1.5, ~1.4, ~1.35, and ~1.3 kb, representing multiple mutant splice variants.
T8298 3982-3986 JJ denotes Wild
T8300 3986-3987 HYPH denotes -
T8299 3987-3991 NN denotes type
T8302 3992-3994 NN denotes RT
T8304 3994-3995 HYPH denotes -
T8303 3995-3998 NN denotes PCR
T8301 3999-4006 NN denotes product
T8305 4007-4010 VBD denotes was
T8306 4011-4012 DT denotes a
T8308 4013-4019 JJ denotes single
T8309 4020-4021 SYM denotes ~
T8310 4021-4024 CD denotes 1.6
T8311 4025-4027 NN denotes kb
T8307 4028-4032 NN denotes band
T8312 4033-4036 CC denotes and
T8313 4037-4043 NN denotes mutant
T8314 4044-4052 NNS denotes products
T8315 4053-4057 VBD denotes were
T8316 4058-4059 SYM denotes ~
T8317 4059-4062 CD denotes 1.6
T8319 4062-4064 , denotes ,
T8320 4064-4065 SYM denotes ~
T8321 4065-4068 CD denotes 1.5
T8322 4068-4070 , denotes ,
T8323 4070-4071 SYM denotes ~
T8324 4071-4074 CD denotes 1.4
T8325 4074-4076 , denotes ,
T8326 4076-4077 SYM denotes ~
T8327 4077-4081 CD denotes 1.35
T8328 4081-4083 , denotes ,
T8329 4083-4086 CC denotes and
T8330 4087-4088 SYM denotes ~
T8331 4088-4091 NN denotes 1.3
T8318 4092-4094 NN denotes kb
T8332 4094-4096 , denotes ,
T8333 4096-4108 VBG denotes representing
T8334 4109-4117 JJ denotes multiple
T8336 4118-4124 NN denotes mutant
T8337 4125-4131 NN denotes splice
T8335 4132-4140 NNS denotes variants
T8338 4140-4141 . denotes .
T8445 4152-4160 NN denotes Battenin
T8447 4161-4164 CC denotes and
T8448 4165-4174 JJ denotes lysosomal
T8449 4175-4178 CC denotes and
T8450 4179-4188 JJ denotes endosomal
T8446 4189-4195 NN denotes marker
T8451 4196-4207 NN denotes co-staining
T8453 4208-4210 IN denotes in
T8454 4211-4215 JJ denotes wild
T8456 4215-4216 HYPH denotes -
T8455 4216-4220 NN denotes type
T8458 4221-4224 CC denotes and
T8459 4225-4235 JJ denotes homozygous
T8460 4236-4246 NN denotes CbCln3Δex7
T8462 4246-4247 HYPH denotes /
T8463 4247-4248 CD denotes 8
T8464 4249-4259 JJ denotes cerebellar
T8461 4260-4269 NN denotes precursor
T8457 4270-4275 NNS denotes cells
T8465 4276-4281 NN denotes Batp1
T8466 4282-4296 NN denotes immunostaining
T8467 4297-4299 IN denotes of
T8468 4300-4304 JJ denotes wild
T8470 4304-4305 HYPH denotes -
T8469 4305-4309 NN denotes type
T8471 4310-4311 -LRB- denotes (
T8472 4311-4317 NN denotes CbCln3
T8473 4317-4318 SYM denotes +
T8474 4318-4319 HYPH denotes /
T8475 4319-4320 SYM denotes +
T8476 4320-4321 -RRB- denotes )
T8477 4322-4325 CC denotes and
T8478 4326-4336 JJ denotes homozygous
T8479 4337-4343 NN denotes mutant
T8481 4344-4345 -LRB- denotes (
T8482 4345-4355 NN denotes CbCln3Δex7
T8484 4355-4356 HYPH denotes /
T8485 4356-4357 CD denotes 8
T8486 4357-4358 HYPH denotes /
T8483 4358-4362 NN denotes Δex7
T8487 4362-4363 HYPH denotes /
T8488 4363-4364 CD denotes 8
T8489 4364-4365 -RRB- denotes )
T8490 4366-4376 JJ denotes cerebellar
T8491 4377-4386 NN denotes precursor
T8480 4387-4392 NNS denotes cells
T8492 4393-4395 VBZ denotes is
T8452 4396-4401 VBN denotes shown
T8493 4401-4403 , denotes ,
T8494 4403-4407 IN denotes with
T8495 4408-4419 NN denotes co-staining
T8496 4420-4423 IN denotes for
T8497 4424-4433 NNS denotes lysosomes
T8498 4434-4435 -LRB- denotes (
T8499 4435-4439 NN denotes Lamp
T8500 4440-4441 CD denotes 1
T8501 4441-4442 -RRB- denotes )
T8502 4442-4444 , denotes ,
T8503 4444-4449 JJ denotes early
T8504 4450-4459 NNS denotes endosomes
T8505 4460-4461 -LRB- denotes (
T8506 4461-4465 NN denotes EEA1
T8507 4465-4466 -RRB- denotes )
T8508 4466-4468 , denotes ,
T8509 4468-4471 CC denotes and
T8510 4472-4476 JJ denotes late
T8511 4477-4486 NNS denotes endosomes
T8512 4487-4488 -LRB- denotes (
T8513 4488-4492 NN denotes Rab7
T8514 4492-4493 -RRB- denotes )
T8515 4493-4494 . denotes .
T8516 4494-4665 sentence denotes Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge).
T8517 4495-4506 JJ denotes Significant
T8518 4507-4514 NN denotes overlap
T8520 4515-4517 IN denotes of
T8521 4518-4523 NN denotes Batp1
T8522 4524-4530 NN denotes signal
T8523 4531-4532 -LRB- denotes (
T8524 4532-4535 JJ denotes red
T8525 4535-4536 -RRB- denotes )
T8526 4537-4541 IN denotes with
T8527 4542-4546 NN denotes EEA1
T8528 4547-4548 -LRB- denotes (
T8530 4548-4553 JJ denotes green
T8531 4553-4555 , denotes ,
T8532 4555-4561 JJ denotes middle
T8529 4562-4568 NNS denotes panels
T8533 4568-4569 -RRB- denotes )
T8534 4570-4573 CC denotes and
T8535 4574-4578 NN denotes Rab7
T8536 4579-4580 -LRB- denotes (
T8538 4580-4585 JJ denotes green
T8539 4585-4587 , denotes ,
T8540 4587-4593 JJ denotes bottom
T8537 4594-4600 NNS denotes panels
T8541 4600-4601 -RRB- denotes )
T8542 4602-4605 MD denotes can
T8543 4606-4608 VB denotes be
T8519 4609-4613 VBN denotes seen
T8544 4614-4616 IN denotes as
T8545 4617-4623 JJ denotes yellow
T8546 4624-4628 WRB denotes when
T8548 4629-4632 DT denotes the
T8550 4633-4636 CD denotes two
T8549 4637-4645 NNS denotes channels
T8551 4646-4649 VBP denotes are
T8547 4650-4656 VBN denotes merged
T8552 4657-4658 -LRB- denotes (
T8553 4658-4663 NNP denotes Merge
T8554 4663-4664 -RRB- denotes )
T8555 4664-4665 . denotes .
T8556 4665-4716 sentence denotes The degree of Batp1 overlap is greatest with Rab7.
T8557 4666-4669 DT denotes The
T8558 4670-4676 NN denotes degree
T8560 4677-4679 IN denotes of
T8561 4680-4685 NN denotes Batp1
T8562 4686-4693 NN denotes overlap
T8559 4694-4696 VBZ denotes is
T8563 4697-4705 JJS denotes greatest
T8564 4706-4710 IN denotes with
T8565 4711-4715 NN denotes Rab7
T8566 4715-4716 . denotes .
T8567 4716-4801 sentence denotes Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen.
T8568 4717-4721 RB denotes Only
T8570 4722-4729 VBN denotes limited
T8569 4730-4737 NN denotes overlap
T8572 4738-4745 IN denotes between
T8573 4746-4751 NN denotes Batp1
T8574 4752-4753 -LRB- denotes (
T8575 4753-4756 JJ denotes red
T8576 4756-4757 -RRB- denotes )
T8577 4758-4761 CC denotes and
T8578 4762-4766 NN denotes Lamp
T8579 4767-4768 CD denotes 1
T8580 4769-4770 -LRB- denotes (
T8582 4770-4775 JJ denotes green
T8583 4775-4777 , denotes ,
T8584 4777-4780 JJ denotes top
T8581 4781-4787 NNS denotes panels
T8585 4787-4788 -RRB- denotes )
T8586 4789-4792 MD denotes can
T8587 4793-4795 VB denotes be
T8571 4796-4800 VBN denotes seen
T8588 4800-4801 . denotes .
T8589 4801-5004 sentence denotes Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels.
T8590 4802-4807 NN denotes Batp1
T8591 4808-4814 NN denotes signal
T8593 4815-4817 IN denotes in
T8594 4818-4828 JJ denotes homozygous
T8596 4829-4839 NN denotes CbCln3Δex7
T8597 4839-4840 HYPH denotes /
T8598 4840-4841 CD denotes 8
T8595 4842-4847 NNS denotes cells
T8592 4848-4850 VBZ denotes is
T8599 4851-4864 RB denotes significantly
T8600 4865-4872 JJ denotes reduced
T8601 4872-4874 , denotes ,
T8602 4874-4877 CC denotes but
T8603 4878-4889 JJ denotes significant
T8604 4890-4897 NN denotes overlap
T8606 4898-4902 IN denotes with
T8607 4903-4907 NN denotes EEA1
T8608 4908-4911 CC denotes and
T8609 4912-4916 NN denotes Rab7
T8610 4916-4918 , denotes ,
T8611 4918-4921 CC denotes and
T8612 4922-4926 RB denotes very
T8613 4927-4933 JJ denotes little
T8615 4934-4938 NNP denotes Lamp
T8616 4939-4940 CD denotes 1
T8614 4941-4948 NN denotes overlap
T8617 4948-4950 , denotes ,
T8618 4950-4953 MD denotes can
T8619 4954-4956 VB denotes be
T8605 4957-4961 VBN denotes seen
T8620 4962-4964 IN denotes as
T8621 4965-4971 JJ denotes yellow
T8622 4972-4974 IN denotes in
T8623 4975-4978 DT denotes the
T8625 4979-4989 JJ denotes respective
T8626 4990-4996 JJ denotes merged
T8624 4997-5003 NNS denotes panels
T8627 5003-5004 . denotes .
T8628 5004-5138 sentence denotes Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells.
T8629 5005-5012 RB denotes Notably
T8631 5012-5014 , denotes ,
T8632 5014-5018 NN denotes Lamp
T8634 5019-5020 CD denotes 1
T8635 5021-5024 CC denotes and
T8636 5025-5029 NN denotes EEA1
T8633 5030-5042 NN denotes localization
T8630 5043-5049 VBP denotes appear
T8637 5050-5057 JJ denotes altered
T8638 5057-5059 , denotes ,
T8639 5059-5062 CC denotes and
T8640 5063-5067 NN denotes Rab7
T8641 5068-5076 NN denotes staining
T8642 5077-5080 VBD denotes was
T8643 5081-5091 RB denotes frequently
T8644 5092-5096 RBR denotes less
T8645 5097-5104 JJ denotes intense
T8646 5105-5107 IN denotes in
T8647 5108-5118 JJ denotes homozygous
T8649 5119-5129 NN denotes CbCln3Δex7
T8650 5129-5130 HYPH denotes /
T8651 5130-5131 CD denotes 8
T8648 5132-5137 NNS denotes cells
T8652 5137-5138 . denotes .
T8653 5138-5240 sentence denotes Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings.
T8654 5139-5143 JJ denotes Wild
T8656 5143-5144 HYPH denotes -
T8655 5144-5148 NN denotes type
T8658 5149-5152 CC denotes and
T8659 5153-5163 JJ denotes homozygous
T8660 5164-5174 NN denotes CbCln3Δex7
T8662 5174-5175 HYPH denotes /
T8663 5175-5176 CD denotes 8
T8664 5177-5185 JJ denotes confocal
T8661 5186-5192 NNS denotes images
T8665 5193-5197 VBD denotes were
T8657 5198-5206 VBN denotes captured
T8666 5207-5211 IN denotes with
T8667 5212-5221 JJ denotes identical
T8669 5222-5230 NN denotes exposure
T8668 5231-5239 NNS denotes settings
T8670 5239-5240 . denotes .
T8671 5240-5260 sentence denotes 60 × magnification.
T8672 5241-5243 CD denotes 60
T8674 5244-5245 SYM denotes ×
T8673 5246-5259 NN denotes magnification
T8675 5259-5260 . denotes .
T2096 5261-5267 IN denotes During
T2098 5268-5281 JJ denotes sub-confluent
T2100 5282-5288 NN denotes growth
T2099 5289-5299 NNS denotes conditions
T2101 5299-5301 , denotes ,
T2102 5301-5308 CC denotes neither
T2104 5309-5313 JJ denotes wild
T2105 5313-5314 HYPH denotes -
T2103 5314-5318 NN denotes type
T2107 5319-5322 CC denotes nor
T2108 5323-5333 JJ denotes homozygous
T2109 5334-5344 NN denotes CbCln3Δex7
T2110 5344-5345 HYPH denotes /
T2111 5345-5346 CD denotes 8
T2106 5347-5352 NNS denotes cells
T2097 5353-5362 VBD denotes displayed
T2112 5363-5379 NN denotes autofluorescence
T2114 5380-5382 CC denotes or
T2115 5383-5390 NN denotes subunit
T2116 5391-5392 NN denotes c
T2117 5393-5402 NN denotes inclusion
T2113 5403-5412 NN denotes formation
T2118 5413-5414 -LRB- denotes (
T2120 5414-5418 NNS denotes data
T2121 5419-5422 RB denotes not
T2119 5423-5428 VBN denotes shown
T2122 5428-5429 -RRB- denotes )
T2123 5429-5430 . denotes .
T2124 5430-5659 sentence denotes However, when cells were aged at confluency (3+ days post-confluency), homozygous CbCln3Δex7/8 cellular subunit c levels were elevated beyond normal wild-type levels by immunostaining (Fig. 4a) and immunoblot analysis (Fig. 4b).
T2125 5431-5438 RB denotes However
T2127 5438-5440 , denotes ,
T2128 5440-5444 WRB denotes when
T2130 5445-5450 NNS denotes cells
T2131 5451-5455 VBD denotes were
T2129 5456-5460 VBN denotes aged
T2132 5461-5463 IN denotes at
T2133 5464-5474 NN denotes confluency
T2134 5475-5476 -LRB- denotes (
T2135 5476-5477 CD denotes 3
T2137 5477-5478 SYM denotes +
T2136 5479-5483 NNS denotes days
T2138 5484-5499 RB denotes post-confluency
T2139 5499-5500 -RRB- denotes )
T2140 5500-5502 , denotes ,
T2141 5502-5512 JJ denotes homozygous
T2143 5513-5523 NN denotes CbCln3Δex7
T2144 5523-5524 HYPH denotes /
T2145 5524-5525 CD denotes 8
T2146 5526-5534 JJ denotes cellular
T2147 5535-5542 NN denotes subunit
T2148 5543-5544 NN denotes c
T2142 5545-5551 NNS denotes levels
T2149 5552-5556 VBD denotes were
T2126 5557-5565 VBN denotes elevated
T2150 5566-5572 IN denotes beyond
T2151 5573-5579 JJ denotes normal
T2153 5580-5584 JJ denotes wild
T2155 5584-5585 HYPH denotes -
T2154 5585-5589 NN denotes type
T2152 5590-5596 NNS denotes levels
T2156 5597-5599 IN denotes by
T2157 5600-5614 NN denotes immunostaining
T2158 5615-5616 -LRB- denotes (
T2160 5616-5620 NN denotes Fig.
T2159 5621-5623 NN denotes 4a
T2161 5623-5624 -RRB- denotes )
T2162 5625-5628 CC denotes and
T2163 5629-5639 NN denotes immunoblot
T2164 5640-5648 NN denotes analysis
T2165 5649-5650 -LRB- denotes (
T2167 5650-5654 NN denotes Fig.
T2166 5655-5657 NN denotes 4b
T2168 5657-5658 -RRB- denotes )
T2169 5658-5659 . denotes .
T2170 5659-5774 sentence denotes Autofluorescent signal sometimes overlapped with subunit c signal, but also was elevated more diffusely (Fig. 4a).
T2171 5660-5675 JJ denotes Autofluorescent
T2172 5676-5682 NN denotes signal
T2174 5683-5692 RB denotes sometimes
T2173 5693-5703 VBD denotes overlapped
T2175 5704-5708 IN denotes with
T2176 5709-5716 NN denotes subunit
T2177 5717-5718 NN denotes c
T2178 5719-5725 NN denotes signal
T2179 5725-5727 , denotes ,
T2180 5727-5730 CC denotes but
T2181 5731-5735 RB denotes also
T2182 5736-5739 VBD denotes was
T2183 5740-5748 JJ denotes elevated
T2184 5749-5753 RBR denotes more
T2185 5754-5763 RB denotes diffusely
T2186 5764-5765 -LRB- denotes (
T2188 5765-5769 NN denotes Fig.
T2187 5770-5772 NN denotes 4a
T2189 5772-5773 -RRB- denotes )
T2190 5773-5774 . denotes .
T2191 5774-6136 sentence denotes Moreover, although multilamellar "fingerprint" profiles were not detected, confluency-aged homozygous CbCln3Δex7/8 cells displayed numerous ultrastructural abnormalities including electron dense inclusions characteristic of lipofuscin and large autophagosomes that contained dense core structures, degenerating mitochondria, and many smaller vesicles (Fig. 4c).
T2192 5775-5783 RB denotes Moreover
T2194 5783-5785 , denotes ,
T2195 5785-5793 IN denotes although
T2197 5794-5807 JJ denotes multilamellar
T2199 5808-5809 `` denotes "
T2200 5809-5820 NN denotes fingerprint
T2201 5820-5821 '' denotes "
T2198 5822-5830 NNS denotes profiles
T2202 5831-5835 VBD denotes were
T2203 5836-5839 RB denotes not
T2196 5840-5848 VBN denotes detected
T2204 5848-5850 , denotes ,
T2205 5850-5860 JJ denotes confluency
T2207 5860-5861 HYPH denotes -
T2206 5861-5865 VBN denotes aged
T2209 5866-5876 JJ denotes homozygous
T2210 5877-5887 NN denotes CbCln3Δex7
T2211 5887-5888 HYPH denotes /
T2212 5888-5889 CD denotes 8
T2208 5890-5895 NNS denotes cells
T2193 5896-5905 VBD denotes displayed
T2213 5906-5914 JJ denotes numerous
T2215 5915-5930 JJ denotes ultrastructural
T2214 5931-5944 NNS denotes abnormalities
T2216 5945-5954 VBG denotes including
T2217 5955-5963 NN denotes electron
T2218 5964-5969 JJ denotes dense
T2219 5970-5980 NNS denotes inclusions
T2220 5981-5995 JJ denotes characteristic
T2221 5996-5998 IN denotes of
T2222 5999-6009 NN denotes lipofuscin
T2223 6010-6013 CC denotes and
T2224 6014-6019 JJ denotes large
T2225 6020-6034 NNS denotes autophagosomes
T2226 6035-6039 WDT denotes that
T2227 6040-6049 VBD denotes contained
T2228 6050-6055 JJ denotes dense
T2230 6056-6060 NN denotes core
T2229 6061-6071 NNS denotes structures
T2231 6071-6073 , denotes ,
T2232 6073-6085 VBG denotes degenerating
T2233 6086-6098 NNS denotes mitochondria
T2234 6098-6100 , denotes ,
T2235 6100-6103 CC denotes and
T2236 6104-6108 JJ denotes many
T2238 6109-6116 JJR denotes smaller
T2237 6117-6125 NNS denotes vesicles
T2239 6126-6127 -LRB- denotes (
T2241 6127-6131 NNP denotes Fig.
T2240 6132-6134 NN denotes 4c
T2242 6134-6135 -RRB- denotes )
T2243 6135-6136 . denotes .
T2244 6136-6255 sentence denotes Inclusion bodies and autophagosomes were infrequently observed in confluency-aged wild-type cultures (data not shown).
T2245 6137-6146 NN denotes Inclusion
T2246 6147-6153 NNS denotes bodies
T2248 6154-6157 CC denotes and
T2249 6158-6172 NNS denotes autophagosomes
T2250 6173-6177 VBD denotes were
T2251 6178-6190 RB denotes infrequently
T2247 6191-6199 VBN denotes observed
T2252 6200-6202 IN denotes in
T2253 6203-6213 NN denotes confluency
T2255 6213-6214 HYPH denotes -
T2254 6214-6218 VBN denotes aged
T2257 6219-6223 JJ denotes wild
T2259 6223-6224 HYPH denotes -
T2258 6224-6228 NN denotes type
T2256 6229-6237 NNS denotes cultures
T2260 6238-6239 -LRB- denotes (
T2262 6239-6243 NNS denotes data
T2263 6244-6247 RB denotes not
T2261 6248-6253 VBN denotes shown
T2264 6253-6254 -RRB- denotes )
T2265 6254-6255 . denotes .
T8805 6266-6273 NN denotes Subunit
T8806 6274-6275 NN denotes c
T8807 6276-6288 NN denotes accumulation
T8808 6289-6291 IN denotes in
T8809 6292-6302 JJ denotes homozygous
T8811 6303-6313 NN denotes CbCln3Δex7
T8812 6313-6314 HYPH denotes /
T8813 6314-6315 CD denotes 8
T8814 6316-6326 JJ denotes cerebellar
T8815 6327-6336 NN denotes precursor
T8810 6337-6342 NNS denotes cells
T8816 6342-6470 sentence denotes a. Subunit c immunostaining and autofluorescence of 7-day confluency-aged wild-type and homozygous CbCln3Δex7/8 cells is shown.
T8817 6343-6344 LS denotes a
T8819 6344-6345 . denotes .
T8820 6346-6353 NN denotes Subunit
T8821 6354-6355 NN denotes c
T8822 6356-6370 NN denotes immunostaining
T8823 6371-6374 CC denotes and
T8824 6375-6391 NN denotes autofluorescence
T8825 6392-6394 IN denotes of
T8826 6395-6396 CD denotes 7
T8828 6396-6397 HYPH denotes -
T8827 6397-6400 NN denotes day
T8829 6401-6411 NN denotes confluency
T8831 6411-6412 HYPH denotes -
T8830 6412-6416 VBN denotes aged
T8833 6417-6421 JJ denotes wild
T8835 6421-6422 HYPH denotes -
T8834 6422-6426 NN denotes type
T8836 6427-6430 CC denotes and
T8837 6431-6441 JJ denotes homozygous
T8838 6442-6452 NN denotes CbCln3Δex7
T8839 6452-6453 HYPH denotes /
T8840 6453-6454 CD denotes 8
T8832 6455-6460 NNS denotes cells
T8841 6461-6463 VBZ denotes is
T8818 6464-6469 VBN denotes shown
T8842 6469-6470 . denotes .
T8843 6470-6566 sentence denotes Wild-type cultures (CbCln3+/+) exhibited limited subunit c immunostaining and autofluorescence.
T8844 6471-6475 JJ denotes Wild
T8846 6475-6476 HYPH denotes -
T8845 6476-6480 NN denotes type
T8847 6481-6489 NNS denotes cultures
T8849 6490-6491 -LRB- denotes (
T8850 6491-6497 NN denotes CbCln3
T8851 6497-6498 SYM denotes +
T8852 6498-6499 HYPH denotes /
T8853 6499-6500 SYM denotes +
T8854 6500-6501 -RRB- denotes )
T8848 6502-6511 VBD denotes exhibited
T8855 6512-6519 JJ denotes limited
T8857 6520-6527 NN denotes subunit
T8856 6528-6529 NN denotes c
T8858 6530-6544 NN denotes immunostaining
T8859 6545-6548 CC denotes and
T8860 6549-6565 NN denotes autofluorescence
T8861 6565-6566 . denotes .
T8862 6566-6638 sentence denotes However, CbCln3Δex7/8/Δex7/8 cells contained numerous subunit c puncta.
T8863 6567-6574 RB denotes However
T8865 6574-6576 , denotes ,
T8866 6576-6586 NN denotes CbCln3Δex7
T8868 6586-6587 HYPH denotes /
T8869 6587-6588 CD denotes 8
T8870 6588-6589 HYPH denotes /
T8867 6589-6593 NN denotes Δex7
T8872 6593-6594 HYPH denotes /
T8873 6594-6595 CD denotes 8
T8871 6596-6601 NNS denotes cells
T8864 6602-6611 VBD denotes contained
T8874 6612-6620 JJ denotes numerous
T8876 6621-6628 NN denotes subunit
T8877 6629-6630 NN denotes c
T8875 6631-6637 NNS denotes puncta
T8878 6637-6638 . denotes .
T8879 6638-6785 sentence denotes Autofluorescence (7 days AF) was also significantly elevated (right panels), although limited overlap with subunit c puncta was observed (arrows).
T8880 6639-6655 NN denotes Autofluorescence
T8882 6656-6657 -LRB- denotes (
T8884 6657-6658 CD denotes 7
T8885 6659-6663 NNS denotes days
T8883 6664-6666 NN denotes AF
T8886 6666-6667 -RRB- denotes )
T8881 6668-6671 VBD denotes was
T8887 6672-6676 RB denotes also
T8888 6677-6690 RB denotes significantly
T8889 6691-6699 VBN denotes elevated
T8890 6700-6701 -LRB- denotes (
T8892 6701-6706 JJ denotes right
T8891 6707-6713 NNS denotes panels
T8893 6713-6714 -RRB- denotes )
T8894 6714-6716 , denotes ,
T8895 6716-6724 IN denotes although
T8897 6725-6732 JJ denotes limited
T8898 6733-6740 NN denotes overlap
T8899 6741-6745 IN denotes with
T8900 6746-6753 NN denotes subunit
T8901 6754-6755 NN denotes c
T8902 6756-6762 NNS denotes puncta
T8903 6763-6766 VBD denotes was
T8896 6767-6775 VBN denotes observed
T8904 6776-6777 -LRB- denotes (
T8905 6777-6783 NNS denotes arrows
T8906 6783-6784 -RRB- denotes )
T8907 6784-6785 . denotes .
T8908 6785-6805 sentence denotes 40 × magnification.
T8909 6786-6788 CD denotes 40
T8911 6789-6790 SYM denotes ×
T8910 6791-6804 NN denotes magnification
T8912 6804-6805 . denotes .
T8913 6805-6908 sentence denotes b. Immunoblot analysis of subunit c protein at sub-confluency or 7-day confluency incubation is shown.
T8914 6806-6807 LS denotes b
T8916 6807-6808 . denotes .
T8917 6809-6819 NN denotes Immunoblot
T8918 6820-6828 NN denotes analysis
T8919 6829-6831 IN denotes of
T8920 6832-6839 NN denotes subunit
T8921 6840-6841 NN denotes c
T8922 6842-6849 NN denotes protein
T8923 6850-6852 IN denotes at
T8924 6853-6867 NN denotes sub-confluency
T8926 6868-6870 CC denotes or
T8927 6871-6872 CD denotes 7
T8929 6872-6873 HYPH denotes -
T8928 6873-6876 NN denotes day
T8930 6877-6887 NN denotes confluency
T8925 6888-6898 NN denotes incubation
T8931 6899-6901 VBZ denotes is
T8915 6902-6907 VBN denotes shown
T8932 6907-6908 . denotes .
T8933 6908-7083 sentence denotes Total protein extracts from sub-confluency wild-type (+/+) and homozygous mutant (Δex7/8/Δex7/8) cultures contained approximately equal levels of subunit c protein (α-sub c).
T8934 6909-6914 JJ denotes Total
T8936 6915-6922 NN denotes protein
T8935 6923-6931 NNS denotes extracts
T8938 6932-6936 IN denotes from
T8939 6937-6951 NN denotes sub-confluency
T8941 6952-6956 JJ denotes wild
T8943 6956-6957 HYPH denotes -
T8942 6957-6961 NN denotes type
T8944 6962-6963 -LRB- denotes (
T8945 6963-6964 SYM denotes +
T8947 6964-6965 HYPH denotes /
T8946 6965-6966 SYM denotes +
T8948 6966-6967 -RRB- denotes )
T8949 6968-6971 CC denotes and
T8950 6972-6982 JJ denotes homozygous
T8951 6983-6989 NN denotes mutant
T8952 6990-6991 -LRB- denotes (
T8953 6991-6995 NN denotes Δex7
T8954 6995-6996 HYPH denotes /
T8955 6996-6997 CD denotes 8
T8956 6997-6998 HYPH denotes /
T8957 6998-7002 NN denotes Δex7
T8958 7002-7003 HYPH denotes /
T8959 7003-7004 CD denotes 8
T8960 7004-7005 -RRB- denotes )
T8940 7006-7014 NNS denotes cultures
T8937 7015-7024 VBD denotes contained
T8961 7025-7038 RB denotes approximately
T8962 7039-7044 JJ denotes equal
T8963 7045-7051 NNS denotes levels
T8964 7052-7054 IN denotes of
T8965 7055-7062 NN denotes subunit
T8966 7063-7064 NN denotes c
T8967 7065-7072 NN denotes protein
T8968 7073-7074 -LRB- denotes (
T8969 7074-7075 NN denotes α
T8971 7075-7076 HYPH denotes -
T8972 7076-7079 JJ denotes sub
T8970 7080-7081 NN denotes c
T8973 7081-7082 -RRB- denotes )
T8974 7082-7083 . denotes .
T8975 7083-7246 sentence denotes 7-day confluency extract from homozygous CbCln3Δex7/8 cells (Δex7/8/Δex7/8) had elevated levels of subunit c protein (~1.5X), relative to wild-type extract (+/+).
T8976 7084-7085 CD denotes 7
T8978 7085-7086 HYPH denotes -
T8977 7086-7089 NN denotes day
T8979 7090-7100 NN denotes confluency
T8980 7101-7108 NN denotes extract
T8982 7109-7113 IN denotes from
T8983 7114-7124 JJ denotes homozygous
T8985 7125-7135 NN denotes CbCln3Δex7
T8986 7135-7136 HYPH denotes /
T8987 7136-7137 CD denotes 8
T8984 7138-7143 NNS denotes cells
T8988 7144-7145 -LRB- denotes (
T8989 7145-7149 NN denotes Δex7
T8990 7149-7150 HYPH denotes /
T8991 7150-7151 CD denotes 8
T8992 7151-7152 HYPH denotes /
T8993 7152-7156 NN denotes Δex7
T8994 7156-7157 HYPH denotes /
T8995 7157-7158 CD denotes 8
T8996 7158-7159 -RRB- denotes )
T8981 7160-7163 VBD denotes had
T8997 7164-7172 VBN denotes elevated
T8998 7173-7179 NNS denotes levels
T8999 7180-7182 IN denotes of
T9000 7183-7190 NN denotes subunit
T9001 7191-7192 NN denotes c
T9002 7193-7200 NN denotes protein
T9003 7201-7202 -LRB- denotes (
T9004 7202-7203 SYM denotes ~
T9005 7203-7206 CD denotes 1.5
T9006 7206-7207 SYM denotes X
T9007 7207-7208 -RRB- denotes )
T9008 7208-7210 , denotes ,
T9009 7210-7218 JJ denotes relative
T9010 7219-7221 IN denotes to
T9011 7222-7226 JJ denotes wild
T9013 7226-7227 HYPH denotes -
T9012 7227-7231 NN denotes type
T9014 7232-7239 NN denotes extract
T9015 7240-7241 -LRB- denotes (
T9016 7241-7242 SYM denotes +
T9018 7242-7243 HYPH denotes /
T9017 7243-7244 SYM denotes +
T9019 7244-7245 -RRB- denotes )
T9020 7245-7246 . denotes .
T9021 7246-7322 sentence denotes Protein levels were normalized to cytochrome c oxidase subunit IV (α-cox4).
T9022 7247-7254 NN denotes Protein
T9023 7255-7261 NNS denotes levels
T9025 7262-7266 VBD denotes were
T9024 7267-7277 VBN denotes normalized
T9026 7278-7280 IN denotes to
T9027 7281-7291 NN denotes cytochrome
T9028 7292-7293 NN denotes c
T9029 7294-7301 NN denotes oxidase
T9030 7302-7309 NN denotes subunit
T9031 7310-7312 CD denotes IV
T9032 7313-7314 -LRB- denotes (
T9033 7314-7315 NN denotes α
T9035 7315-7316 HYPH denotes -
T9034 7316-7320 NN denotes cox4
T9036 7320-7321 -RRB- denotes )
T9037 7321-7322 . denotes .
T9038 7322-7417 sentence denotes c. TEM analysis of inclusions in 7-day confluency-aged homozygous CbCln3Δex7/8 cells is shown.
T9039 7323-7324 LS denotes c
T9041 7324-7325 . denotes .
T9042 7326-7329 NN denotes TEM
T9043 7330-7338 NN denotes analysis
T9044 7339-7341 IN denotes of
T9045 7342-7352 NNS denotes inclusions
T9046 7353-7355 IN denotes in
T9047 7356-7357 CD denotes 7
T9049 7357-7358 HYPH denotes -
T9048 7358-7361 NN denotes day
T9050 7362-7372 NN denotes confluency
T9052 7372-7373 HYPH denotes -
T9051 7373-7377 VBN denotes aged
T9054 7378-7388 JJ denotes homozygous
T9055 7389-7399 NN denotes CbCln3Δex7
T9056 7399-7400 HYPH denotes /
T9057 7400-7401 CD denotes 8
T9053 7402-7407 NNS denotes cells
T9058 7408-7410 VBZ denotes is
T9040 7411-7416 VBN denotes shown
T9059 7416-7417 . denotes .
T9060 7417-7607 sentence denotes A large autophagosome contained by double membrane (arrows) is filled with degenerating mitochondria (Md), electron dense cores (left and right of *) and other smaller vesicular structures.
T9061 7418-7419 DT denotes A
T9063 7420-7425 JJ denotes large
T9062 7426-7439 NN denotes autophagosome
T9065 7440-7449 VBN denotes contained
T9066 7450-7452 IN denotes by
T9067 7453-7459 JJ denotes double
T9068 7460-7468 NN denotes membrane
T9069 7469-7470 -LRB- denotes (
T9070 7470-7476 NNS denotes arrows
T9071 7476-7477 -RRB- denotes )
T9072 7478-7480 VBZ denotes is
T9064 7481-7487 VBN denotes filled
T9073 7488-7492 IN denotes with
T9074 7493-7505 VBG denotes degenerating
T9075 7506-7518 NNS denotes mitochondria
T9076 7519-7520 -LRB- denotes (
T9077 7520-7522 NN denotes Md
T9078 7522-7523 -RRB- denotes )
T9079 7523-7525 , denotes ,
T9080 7525-7533 NN denotes electron
T9082 7534-7539 JJ denotes dense
T9081 7540-7545 NNS denotes cores
T9083 7546-7547 -LRB- denotes (
T9084 7547-7551 JJ denotes left
T9085 7552-7555 CC denotes and
T9086 7556-7561 JJ denotes right
T9087 7562-7564 IN denotes of
T9088 7565-7566 SYM denotes *
T9089 7566-7567 -RRB- denotes )
T9090 7568-7571 CC denotes and
T9091 7572-7577 JJ denotes other
T9093 7578-7585 JJR denotes smaller
T9094 7586-7595 JJ denotes vesicular
T9092 7596-7606 NNS denotes structures
T9095 7606-7607 . denotes .
T9096 7607-7693 sentence denotes A large electron-dense inclusion, with a lipofuscin (Ln) appearance, is also present.
T9097 7608-7609 DT denotes A
T9099 7610-7615 JJ denotes large
T9100 7616-7624 NN denotes electron
T9102 7624-7625 HYPH denotes -
T9101 7625-7630 JJ denotes dense
T9098 7631-7640 NN denotes inclusion
T9104 7640-7642 , denotes ,
T9105 7642-7646 IN denotes with
T9106 7647-7648 DT denotes a
T9108 7649-7659 NN denotes lipofuscin
T9109 7660-7661 -LRB- denotes (
T9110 7661-7663 NN denotes Ln
T9111 7663-7664 -RRB- denotes )
T9107 7665-7675 NN denotes appearance
T9112 7675-7677 , denotes ,
T9103 7677-7679 VBZ denotes is
T9113 7680-7684 RB denotes also
T9114 7685-7692 JJ denotes present
T9115 7692-7693 . denotes .
T9116 7693-7710 sentence denotes M, mitochondria.
T9117 7694-7695 NN denotes M
T9118 7695-7697 , denotes ,
T9119 7697-7709 NNS denotes mitochondria
T9120 7709-7710 . denotes .
T9121 7710-7734 sentence denotes 10,000 × magnification.
T9122 7711-7717 CD denotes 10,000
T9124 7718-7719 SYM denotes ×
T9123 7720-7733 NN denotes magnification
T9125 7733-7734 . denotes .
T2526 7736-7746 JJ denotes Homozygous
T2528 7747-7757 NN denotes CbCln3Δex7
T2529 7757-7758 HYPH denotes /
T2530 7758-7759 CD denotes 8
T2527 7760-7765 NNS denotes cells
T2532 7766-7769 CC denotes and
T2533 7770-7778 NN denotes Cln3Δex7
T2535 7778-7779 HYPH denotes /
T2536 7779-7780 CD denotes 8
T2537 7781-7786 VB denotes knock
T2538 7786-7787 HYPH denotes -
T2539 7787-7789 RP denotes in
T2534 7790-7794 NNS denotes mice
T2531 7795-7802 VBP denotes process
T2540 7803-7812 NN denotes cathepsin
T2541 7813-7814 NN denotes D
T2542 7815-7825 RB denotes abnormally
T2543 7825-8018 sentence denotes We next investigated the basis for subunit c accumulation, testing the hypothesis that cathepsin D is abnormal since it is required for ATP synthase subunit c degradation in the lysosome [13].
T2544 7826-7828 PRP denotes We
T2546 7829-7833 RB denotes next
T2545 7834-7846 VBD denotes investigated
T2547 7847-7850 DT denotes the
T2548 7851-7856 NN denotes basis
T2549 7857-7860 IN denotes for
T2550 7861-7868 NN denotes subunit
T2551 7869-7870 NN denotes c
T2552 7871-7883 NN denotes accumulation
T2553 7883-7885 , denotes ,
T2554 7885-7892 VBG denotes testing
T2555 7893-7896 DT denotes the
T2556 7897-7907 NN denotes hypothesis
T2557 7908-7912 IN denotes that
T2559 7913-7922 NN denotes cathepsin
T2560 7923-7924 NN denotes D
T2558 7925-7927 VBZ denotes is
T2561 7928-7936 JJ denotes abnormal
T2562 7937-7942 IN denotes since
T2564 7943-7945 PRP denotes it
T2563 7946-7948 VBZ denotes is
T2565 7949-7957 JJ denotes required
T2566 7958-7961 IN denotes for
T2567 7962-7965 NN denotes ATP
T2569 7966-7974 NN denotes synthase
T2570 7975-7982 NN denotes subunit
T2571 7983-7984 NN denotes c
T2568 7985-7996 NN denotes degradation
T2572 7997-7999 IN denotes in
T2573 8000-8003 DT denotes the
T2574 8004-8012 NN denotes lysosome
T2575 8013-8014 -LRB- denotes [
T2576 8014-8016 CD denotes 13
T2577 8016-8017 -RRB- denotes ]
T2578 8017-8018 . denotes .
T2579 8018-8220 sentence denotes We first tested cathepsin D transport and processing in homozygous CbCln3Δex7/8 cells and Cln3Δex7/8 mice using anti-cathepsin D antibody that recognizes unprocessed and processed cathepsin D isoforms.
T2580 8019-8021 PRP denotes We
T2582 8022-8027 RB denotes first
T2581 8028-8034 VBD denotes tested
T2583 8035-8044 NN denotes cathepsin
T2584 8045-8046 NN denotes D
T2585 8047-8056 NN denotes transport
T2586 8057-8060 CC denotes and
T2587 8061-8071 NN denotes processing
T2588 8072-8074 IN denotes in
T2589 8075-8085 JJ denotes homozygous
T2591 8086-8096 NN denotes CbCln3Δex7
T2592 8096-8097 HYPH denotes /
T2593 8097-8098 CD denotes 8
T2590 8099-8104 NNS denotes cells
T2594 8105-8108 CC denotes and
T2595 8109-8117 NN denotes Cln3Δex7
T2597 8117-8118 HYPH denotes /
T2598 8118-8119 CD denotes 8
T2596 8120-8124 NNS denotes mice
T2599 8125-8130 VBG denotes using
T2600 8131-8145 JJ denotes anti-cathepsin
T2601 8146-8147 NN denotes D
T2602 8148-8156 NN denotes antibody
T2603 8157-8161 WDT denotes that
T2604 8162-8172 VBZ denotes recognizes
T2605 8173-8184 JJ denotes unprocessed
T2607 8185-8188 CC denotes and
T2608 8189-8198 VBN denotes processed
T2609 8199-8208 NN denotes cathepsin
T2610 8209-8210 NN denotes D
T2606 8211-8219 NNS denotes isoforms
T2611 8219-8220 . denotes .
T2612 8220-8469 sentence denotes Immunostaining of wild-type and homozygous CbCln3Δex7/8 cells revealed a perinuclear and punctate vesicular cathepsin D distribution, consistent with its transport and processing through the secretory pathway and delivery to the lysosome (Fig. 5a).
T2613 8221-8235 NN denotes Immunostaining
T2615 8236-8238 IN denotes of
T2616 8239-8243 JJ denotes wild
T2618 8243-8244 HYPH denotes -
T2617 8244-8248 NN denotes type
T2620 8249-8252 CC denotes and
T2621 8253-8263 JJ denotes homozygous
T2622 8264-8274 NN denotes CbCln3Δex7
T2623 8274-8275 HYPH denotes /
T2624 8275-8276 CD denotes 8
T2619 8277-8282 NNS denotes cells
T2614 8283-8291 VBD denotes revealed
T2625 8292-8293 DT denotes a
T2627 8294-8305 JJ denotes perinuclear
T2628 8306-8309 CC denotes and
T2629 8310-8318 JJ denotes punctate
T2630 8319-8328 JJ denotes vesicular
T2631 8329-8338 NN denotes cathepsin
T2632 8339-8340 NN denotes D
T2626 8341-8353 NN denotes distribution
T2633 8353-8355 , denotes ,
T2634 8355-8365 JJ denotes consistent
T2635 8366-8370 IN denotes with
T2636 8371-8374 PRP$ denotes its
T2637 8375-8384 NN denotes transport
T2638 8385-8388 CC denotes and
T2639 8389-8399 NN denotes processing
T2640 8400-8407 IN denotes through
T2641 8408-8411 DT denotes the
T2643 8412-8421 JJ denotes secretory
T2642 8422-8429 NN denotes pathway
T2644 8430-8433 CC denotes and
T2645 8434-8442 NN denotes delivery
T2646 8443-8445 IN denotes to
T2647 8446-8449 DT denotes the
T2648 8450-8458 NN denotes lysosome
T2649 8459-8460 -LRB- denotes (
T2651 8460-8464 NN denotes Fig.
T2650 8465-8467 NN denotes 5a
T2652 8467-8468 -RRB- denotes )
T2653 8468-8469 . denotes .
T2654 8469-8612 sentence denotes However, in homozygous CbCln3Δex7/8 cells, cathepsin D distribution was less vesicular and more perinuclear-clustered than in wild-type cells.
T2655 8470-8477 RB denotes However
T2657 8477-8479 , denotes ,
T2658 8479-8481 IN denotes in
T2659 8482-8492 JJ denotes homozygous
T2661 8493-8503 NN denotes CbCln3Δex7
T2662 8503-8504 HYPH denotes /
T2663 8504-8505 CD denotes 8
T2660 8506-8511 NNS denotes cells
T2664 8511-8513 , denotes ,
T2665 8513-8522 NN denotes cathepsin
T2666 8523-8524 NN denotes D
T2667 8525-8537 NN denotes distribution
T2656 8538-8541 VBD denotes was
T2668 8542-8546 RBR denotes less
T2669 8547-8556 JJ denotes vesicular
T2670 8557-8560 CC denotes and
T2671 8561-8565 RBR denotes more
T2673 8566-8577 JJ denotes perinuclear
T2674 8577-8578 HYPH denotes -
T2672 8578-8587 VBN denotes clustered
T2675 8588-8592 IN denotes than
T2676 8593-8595 IN denotes in
T2677 8596-8600 JJ denotes wild
T2679 8600-8601 HYPH denotes -
T2678 8601-8605 NN denotes type
T2680 8606-8611 NNS denotes cells
T2681 8611-8612 . denotes .
T2682 8612-8758 sentence denotes Immunoblots of homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts also showed altered relative levels of cathepsin D isoforms (Fig. 5b).
T2683 8613-8624 NNS denotes Immunoblots
T2685 8625-8627 IN denotes of
T2686 8628-8638 JJ denotes homozygous
T2688 8639-8649 NN denotes CbCln3Δex7
T2690 8649-8650 HYPH denotes /
T2691 8650-8651 CD denotes 8
T2689 8652-8656 NN denotes cell
T2692 8657-8660 CC denotes and
T2693 8661-8669 NN denotes Cln3Δex7
T2695 8669-8670 HYPH denotes /
T2696 8670-8671 CD denotes 8
T2694 8672-8678 NN denotes tissue
T2687 8679-8687 NNS denotes extracts
T2697 8688-8692 RB denotes also
T2684 8693-8699 VBD denotes showed
T2698 8700-8707 VBN denotes altered
T2700 8708-8716 JJ denotes relative
T2699 8717-8723 NNS denotes levels
T2701 8724-8726 IN denotes of
T2702 8727-8736 NN denotes cathepsin
T2703 8737-8738 NN denotes D
T2704 8739-8747 NNS denotes isoforms
T2705 8748-8749 -LRB- denotes (
T2707 8749-8753 NN denotes Fig.
T2706 8754-8756 NN denotes 5b
T2708 8756-8757 -RRB- denotes )
T2709 8757-8758 . denotes .
T2710 8758-8992 sentence denotes Cathepsin D isoforms, identified by relative molecular weights, represent the ~45 kDa precursor, the ~43 kDa intermediate single chain form of the enzyme, and the 31 kDa heavy chain of the double-chain form of the mature enzyme [14].
T2711 8759-8768 NN denotes Cathepsin
T2712 8769-8770 NN denotes D
T2713 8771-8779 NNS denotes isoforms
T2715 8779-8781 , denotes ,
T2716 8781-8791 VBN denotes identified
T2717 8792-8794 IN denotes by
T2718 8795-8803 JJ denotes relative
T2720 8804-8813 JJ denotes molecular
T2719 8814-8821 NNS denotes weights
T2721 8821-8823 , denotes ,
T2714 8823-8832 VBP denotes represent
T2722 8833-8836 DT denotes the
T2724 8837-8838 SYM denotes ~
T2725 8838-8840 CD denotes 45
T2726 8841-8844 NN denotes kDa
T2723 8845-8854 NN denotes precursor
T2727 8854-8856 , denotes ,
T2728 8856-8859 DT denotes the
T2730 8860-8861 SYM denotes ~
T2731 8861-8863 CD denotes 43
T2732 8864-8867 NN denotes kDa
T2733 8868-8880 JJ denotes intermediate
T2734 8881-8887 JJ denotes single
T2735 8888-8893 NN denotes chain
T2729 8894-8898 NN denotes form
T2736 8899-8901 IN denotes of
T2737 8902-8905 DT denotes the
T2738 8906-8912 NN denotes enzyme
T2739 8912-8914 , denotes ,
T2740 8914-8917 CC denotes and
T2741 8918-8921 DT denotes the
T2743 8922-8924 CD denotes 31
T2744 8925-8928 NN denotes kDa
T2745 8929-8934 JJ denotes heavy
T2742 8935-8940 NN denotes chain
T2746 8941-8943 IN denotes of
T2747 8944-8947 DT denotes the
T2749 8948-8954 JJ denotes double
T2751 8954-8955 HYPH denotes -
T2750 8955-8960 NN denotes chain
T2748 8961-8965 NN denotes form
T2752 8966-8968 IN denotes of
T2753 8969-8972 DT denotes the
T2755 8973-8979 JJ denotes mature
T2754 8980-8986 NN denotes enzyme
T2756 8987-8988 -LRB- denotes [
T2757 8988-8990 CD denotes 14
T2758 8990-8991 -RRB- denotes ]
T2759 8991-8992 . denotes .
T2760 8992-9176 sentence denotes In homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts, the precursor and heavy chains were reduced, and the single chain was slightly elevated compared to wild-type extracts.
T2761 8993-8995 IN denotes In
T2763 8996-9006 JJ denotes homozygous
T2765 9007-9017 NN denotes CbCln3Δex7
T2767 9017-9018 HYPH denotes /
T2768 9018-9019 CD denotes 8
T2766 9020-9024 NN denotes cell
T2769 9025-9028 CC denotes and
T2770 9029-9037 NN denotes Cln3Δex7
T2772 9037-9038 HYPH denotes /
T2773 9038-9039 CD denotes 8
T2771 9040-9046 NN denotes tissue
T2764 9047-9055 NNS denotes extracts
T2774 9055-9057 , denotes ,
T2775 9057-9060 DT denotes the
T2776 9061-9070 NN denotes precursor
T2777 9071-9074 CC denotes and
T2778 9075-9080 JJ denotes heavy
T2779 9081-9087 NNS denotes chains
T2780 9088-9092 VBD denotes were
T2762 9093-9100 VBN denotes reduced
T2781 9100-9102 , denotes ,
T2782 9102-9105 CC denotes and
T2783 9106-9109 DT denotes the
T2785 9110-9116 JJ denotes single
T2784 9117-9122 NN denotes chain
T2786 9123-9126 VBD denotes was
T2787 9127-9135 RB denotes slightly
T2788 9136-9144 JJ denotes elevated
T2789 9145-9153 VBN denotes compared
T2790 9154-9156 IN denotes to
T2791 9157-9161 JJ denotes wild
T2793 9161-9162 HYPH denotes -
T2792 9162-9166 NN denotes type
T2794 9167-9175 NNS denotes extracts
T2795 9175-9176 . denotes .
T2796 9176-9295 sentence denotes The cellular growth media did not contain altered levels of cathepsin D, indicating enzyme secretion was not affected.
T2797 9177-9180 DT denotes The
T2799 9181-9189 JJ denotes cellular
T2800 9190-9196 NN denotes growth
T2798 9197-9202 NNS denotes media
T2802 9203-9206 VBD denotes did
T2803 9207-9210 RB denotes not
T2801 9211-9218 VB denotes contain
T2804 9219-9226 VBN denotes altered
T2805 9227-9233 NNS denotes levels
T2806 9234-9236 IN denotes of
T2807 9237-9246 NN denotes cathepsin
T2808 9247-9248 NN denotes D
T2809 9248-9250 , denotes ,
T2810 9250-9260 VBG denotes indicating
T2811 9261-9267 NN denotes enzyme
T2812 9268-9277 NN denotes secretion
T2814 9278-9281 VBD denotes was
T2815 9282-9285 RB denotes not
T2813 9286-9294 VBN denotes affected
T2816 9294-9295 . denotes .
T2817 9295-9450 sentence denotes Heterozygous Cln3Δex7/8 mice and CbCln3Δex7/8 cells were indistinguishable from wild-type, as expected for a recessive disease phenotype (data not shown).
T2818 9296-9308 JJ denotes Heterozygous
T2820 9309-9317 NN denotes Cln3Δex7
T2821 9317-9318 HYPH denotes /
T2822 9318-9319 CD denotes 8
T2819 9320-9324 NNS denotes mice
T2824 9325-9328 CC denotes and
T2825 9329-9339 NN denotes CbCln3Δex7
T2827 9339-9340 HYPH denotes /
T2828 9340-9341 CD denotes 8
T2826 9342-9347 NNS denotes cells
T2823 9348-9352 VBD denotes were
T2829 9353-9370 JJ denotes indistinguishable
T2830 9371-9375 IN denotes from
T2831 9376-9380 JJ denotes wild
T2833 9380-9381 HYPH denotes -
T2832 9381-9385 NN denotes type
T2834 9385-9387 , denotes ,
T2835 9387-9389 IN denotes as
T2836 9390-9398 VBN denotes expected
T2837 9399-9402 IN denotes for
T2838 9403-9404 DT denotes a
T2840 9405-9414 JJ denotes recessive
T2841 9415-9422 NN denotes disease
T2839 9423-9432 NN denotes phenotype
T2842 9433-9434 -LRB- denotes (
T2844 9434-9438 NNS denotes data
T2845 9439-9442 RB denotes not
T2843 9443-9448 VBN denotes shown
T2846 9448-9449 -RRB- denotes )
T2847 9449-9450 . denotes .
T2848 9450-9451 sentence denotes
T9254 9461-9470 NN denotes Cathepsin
T9255 9471-9472 NN denotes D
T9256 9473-9485 NN denotes localization
T9257 9486-9489 CC denotes and
T9258 9490-9500 NN denotes processing
T9259 9501-9503 IN denotes in
T9260 9504-9508 JJ denotes wild
T9262 9508-9509 HYPH denotes -
T9261 9509-9513 NN denotes type
T9264 9514-9517 CC denotes and
T9265 9518-9528 JJ denotes homozygous
T9266 9529-9539 NN denotes CbCln3Δex7
T9267 9539-9540 HYPH denotes /
T9268 9540-9541 CD denotes 8
T9263 9542-9547 NNS denotes cells
T9269 9547-9731 sentence denotes a. Immunostaining of wild-type and homozygous CbCln3Δex7/8 precursor cells with anti-cathepsin D antibody, recognizing unprocessed and processed forms of cathepsin D protein is shown.
T9270 9548-9549 LS denotes a
T9272 9549-9550 . denotes .
T9273 9551-9565 NN denotes Immunostaining
T9274 9566-9568 IN denotes of
T9275 9569-9573 JJ denotes wild
T9277 9573-9574 HYPH denotes -
T9276 9574-9578 NN denotes type
T9279 9579-9582 CC denotes and
T9280 9583-9593 JJ denotes homozygous
T9281 9594-9604 NN denotes CbCln3Δex7
T9282 9604-9605 HYPH denotes /
T9283 9605-9606 CD denotes 8
T9284 9607-9616 NN denotes precursor
T9278 9617-9622 NNS denotes cells
T9285 9623-9627 IN denotes with
T9286 9628-9642 JJ denotes anti-cathepsin
T9287 9643-9644 NN denotes D
T9288 9645-9653 NN denotes antibody
T9289 9653-9655 , denotes ,
T9290 9655-9666 VBG denotes recognizing
T9291 9667-9678 JJ denotes unprocessed
T9293 9679-9682 CC denotes and
T9294 9683-9692 VBN denotes processed
T9292 9693-9698 NNS denotes forms
T9295 9699-9701 IN denotes of
T9296 9702-9711 NN denotes cathepsin
T9297 9712-9713 NN denotes D
T9298 9714-9721 NN denotes protein
T9299 9722-9724 VBZ denotes is
T9271 9725-9730 VBN denotes shown
T9300 9730-9731 . denotes .
T9301 9731-9817 sentence denotes CbCln3+/+ cells (left panel) exhibited a perinuclear and cytoplasmic punctate signal.
T9302 9732-9738 NN denotes CbCln3
T9304 9738-9739 SYM denotes +
T9305 9739-9740 HYPH denotes /
T9306 9740-9741 SYM denotes +
T9303 9742-9747 NNS denotes cells
T9308 9748-9749 -LRB- denotes (
T9310 9749-9753 JJ denotes left
T9309 9754-9759 NN denotes panel
T9311 9759-9760 -RRB- denotes )
T9307 9761-9770 VBD denotes exhibited
T9312 9771-9772 DT denotes a
T9314 9773-9784 JJ denotes perinuclear
T9315 9785-9788 CC denotes and
T9316 9789-9800 JJ denotes cytoplasmic
T9317 9801-9809 JJ denotes punctate
T9313 9810-9816 NN denotes signal
T9318 9816-9817 . denotes .
T9319 9817-9989 sentence denotes Cathepsin D signal in homozygous CbCln3Δex7/8 cells (right panel) was more often perinuclear, with less cytoplasmic punctate signal, compared to wild-type CbCln3+/+ cells.
T9320 9818-9827 NN denotes Cathepsin
T9321 9828-9829 NN denotes D
T9322 9830-9836 NN denotes signal
T9324 9837-9839 IN denotes in
T9325 9840-9850 JJ denotes homozygous
T9327 9851-9861 NN denotes CbCln3Δex7
T9328 9861-9862 HYPH denotes /
T9329 9862-9863 CD denotes 8
T9326 9864-9869 NNS denotes cells
T9330 9870-9871 -LRB- denotes (
T9332 9871-9876 JJ denotes right
T9331 9877-9882 NN denotes panel
T9333 9882-9883 -RRB- denotes )
T9323 9884-9887 VBD denotes was
T9334 9888-9892 RBR denotes more
T9335 9893-9898 RB denotes often
T9336 9899-9910 JJ denotes perinuclear
T9337 9910-9912 , denotes ,
T9338 9912-9916 IN denotes with
T9339 9917-9921 RBR denotes less
T9341 9922-9933 JJ denotes cytoplasmic
T9342 9934-9942 NN denotes punctate
T9340 9943-9949 NN denotes signal
T9343 9949-9951 , denotes ,
T9344 9951-9959 VBN denotes compared
T9345 9960-9962 IN denotes to
T9346 9963-9967 JJ denotes wild
T9348 9967-9968 HYPH denotes -
T9347 9968-9972 NN denotes type
T9350 9973-9979 NN denotes CbCln3
T9351 9979-9980 SYM denotes +
T9352 9980-9981 HYPH denotes /
T9353 9981-9982 SYM denotes +
T9349 9983-9988 NNS denotes cells
T9354 9988-9989 . denotes .
T9355 9989-10009 sentence denotes 40 × magnification.
T9356 9990-9992 CD denotes 40
T9358 9993-9994 SYM denotes ×
T9357 9995-10008 NN denotes magnification
T9359 10008-10009 . denotes .
T9360 10009-10154 sentence denotes b. α-Cathepsin D-probed immunoblots of total wild-type versus homozygous Cln3Δex7/8 knock-in tissue or CbCln3Δex7/8 cellular extracts are shown.
T9361 10010-10011 LS denotes b
T9363 10011-10012 . denotes .
T9364 10013-10014 NN denotes α
T9366 10014-10015 HYPH denotes -
T9367 10015-10024 NN denotes Cathepsin
T9365 10025-10026 NN denotes D
T9369 10026-10027 HYPH denotes -
T9368 10027-10033 VBN denotes probed
T9370 10034-10045 NNS denotes immunoblots
T9371 10046-10048 IN denotes of
T9372 10049-10054 JJ denotes total
T9374 10055-10059 JJ denotes wild
T9376 10059-10060 HYPH denotes -
T9375 10060-10064 NN denotes type
T9377 10065-10071 CC denotes versus
T9378 10072-10082 JJ denotes homozygous
T9379 10083-10091 NN denotes Cln3Δex7
T9381 10091-10092 HYPH denotes /
T9382 10092-10093 CD denotes 8
T9380 10094-10099 VB denotes knock
T9383 10099-10100 HYPH denotes -
T9384 10100-10102 RP denotes in
T9373 10103-10109 NN denotes tissue
T9385 10110-10112 CC denotes or
T9386 10113-10123 NN denotes CbCln3Δex7
T9388 10123-10124 HYPH denotes /
T9389 10124-10125 CD denotes 8
T9390 10126-10134 JJ denotes cellular
T9387 10135-10143 NNS denotes extracts
T9391 10144-10147 VBP denotes are
T9362 10148-10153 VBN denotes shown
T9392 10153-10154 . denotes .
T9393 10154-10367 sentence denotes The ~45 kDa cathepsin D band, representing precursor, was the predominant band in wild-type (wt) tissue and cellular extracts, with lower levels of mature enzyme (single chain, ~43 kDa, and heavy chain, ~31 kDa).
T9394 10155-10158 DT denotes The
T9396 10159-10160 SYM denotes ~
T9397 10160-10162 CD denotes 45
T9398 10163-10166 NN denotes kDa
T9399 10167-10176 NN denotes cathepsin
T9400 10177-10178 NN denotes D
T9395 10179-10183 NN denotes band
T9402 10183-10185 , denotes ,
T9403 10185-10197 VBG denotes representing
T9404 10198-10207 NN denotes precursor
T9405 10207-10209 , denotes ,
T9401 10209-10212 VBD denotes was
T9406 10213-10216 DT denotes the
T9408 10217-10228 JJ denotes predominant
T9407 10229-10233 NN denotes band
T9409 10234-10236 IN denotes in
T9410 10237-10241 JJ denotes wild
T9412 10241-10242 HYPH denotes -
T9411 10242-10246 NN denotes type
T9413 10247-10248 -LRB- denotes (
T9414 10248-10250 NN denotes wt
T9415 10250-10251 -RRB- denotes )
T9416 10252-10258 NN denotes tissue
T9417 10259-10262 CC denotes and
T9418 10263-10271 JJ denotes cellular
T9419 10272-10280 NNS denotes extracts
T9420 10280-10282 , denotes ,
T9421 10282-10286 IN denotes with
T9422 10287-10292 JJR denotes lower
T9423 10293-10299 NNS denotes levels
T9424 10300-10302 IN denotes of
T9425 10303-10309 JJ denotes mature
T9426 10310-10316 NN denotes enzyme
T9427 10317-10318 -LRB- denotes (
T9429 10318-10324 JJ denotes single
T9430 10325-10330 NN denotes chain
T9431 10330-10332 , denotes ,
T9432 10332-10333 SYM denotes ~
T9433 10333-10335 CD denotes 43
T9428 10336-10339 NN denotes kDa
T9434 10339-10341 , denotes ,
T9435 10341-10344 CC denotes and
T9436 10345-10350 JJ denotes heavy
T9437 10351-10356 NN denotes chain
T9439 10356-10358 , denotes ,
T9440 10358-10359 SYM denotes ~
T9441 10359-10361 CD denotes 31
T9438 10362-10365 NN denotes kDa
T9442 10365-10366 -RRB- denotes )
T9443 10366-10367 . denotes .
T9444 10367-10584 sentence denotes Conversely, homozygous Cln3Δex7/8 and CbCln3Δex7/8 mutant (m) extracts exhibited reduced levels of precursor and heavy chain of the double-chain form of the enzyme, with elevated levels of single-chain mature enzyme.
T9445 10368-10378 RB denotes Conversely
T9447 10378-10380 , denotes ,
T9448 10380-10390 JJ denotes homozygous
T9450 10391-10399 NN denotes Cln3Δex7
T9451 10399-10400 HYPH denotes /
T9452 10400-10401 CD denotes 8
T9453 10402-10405 CC denotes and
T9454 10406-10416 NN denotes CbCln3Δex7
T9455 10416-10417 HYPH denotes /
T9456 10417-10418 CD denotes 8
T9457 10419-10425 NN denotes mutant
T9458 10426-10427 -LRB- denotes (
T9459 10427-10428 NN denotes m
T9460 10428-10429 -RRB- denotes )
T9449 10430-10438 NNS denotes extracts
T9446 10439-10448 VBD denotes exhibited
T9461 10449-10456 VBN denotes reduced
T9462 10457-10463 NNS denotes levels
T9463 10464-10466 IN denotes of
T9464 10467-10476 NN denotes precursor
T9465 10477-10480 CC denotes and
T9466 10481-10486 JJ denotes heavy
T9467 10487-10492 NN denotes chain
T9468 10493-10495 IN denotes of
T9469 10496-10499 DT denotes the
T9471 10500-10506 JJ denotes double
T9473 10506-10507 HYPH denotes -
T9472 10507-10512 NN denotes chain
T9470 10513-10517 NN denotes form
T9474 10518-10520 IN denotes of
T9475 10521-10524 DT denotes the
T9476 10525-10531 NN denotes enzyme
T9477 10531-10533 , denotes ,
T9478 10533-10537 IN denotes with
T9479 10538-10546 JJ denotes elevated
T9480 10547-10553 NNS denotes levels
T9481 10554-10556 IN denotes of
T9482 10557-10563 JJ denotes single
T9484 10563-10564 HYPH denotes -
T9483 10564-10569 NN denotes chain
T9486 10570-10576 JJ denotes mature
T9485 10577-10583 NN denotes enzyme
T9487 10583-10584 . denotes .
T2850 10585-10588 DT denotes The
T2849 10585-10604 sentence denotes The impact of the a
T2851 10589-10590 NN denotes i
T2852 10590-10591 JJ denotes m
T2853 10591-10592 NN denotes p
T2854 10592-10593 CC denotes a
T2855 10593-10594 JJ denotes c
T2856 10594-10595 NNS denotes t
T2857 10595-10596 , denotes
T2858 10596-10597 JJR denotes o
T2859 10597-10598 IN denotes f
T2860 10599-10600 JJ denotes t
T2861 10600-10601 NN denotes h
T2862 10601-10602 JJ denotes e
T2863 10603-10604 NN denotes a
T2865 10604-10605 RB denotes l
T2864 10604-10616 sentence denotes ltered cathe
T2868 10605-10606 NN denotes t
T2867 10606-10608 NNS denotes er
T2866 10608-10610 VBD denotes ed
T2869 10611-10612 VBN denotes c
T2870 10612-10613 CC denotes a
T2871 10613-10616 DT denotes the
T2873 10616-10617 NN denotes p
T2872 10616-10759 sentence denotes psin D processing on enzymatic activity was next tested to determine if altered enzymatic activity accounts for inefficient subunit c turnover.
T2876 10617-10620 NN denotes sin
T2877 10621-10622 NN denotes D
T2875 10623-10633 NN denotes processing
T2878 10634-10636 IN denotes on
T2879 10637-10646 JJ denotes enzymatic
T2880 10647-10655 NN denotes activity
T2881 10656-10659 VBD denotes was
T2882 10660-10664 RB denotes next
T2874 10665-10671 VBN denotes tested
T2883 10672-10674 TO denotes to
T2884 10675-10684 VB denotes determine
T2885 10685-10687 IN denotes if
T2887 10688-10695 VBN denotes altered
T2889 10696-10705 JJ denotes enzymatic
T2888 10706-10714 NN denotes activity
T2886 10715-10723 VBZ denotes accounts
T2890 10724-10727 IN denotes for
T2891 10728-10739 JJ denotes inefficient
T2893 10740-10747 NN denotes subunit
T2894 10748-10749 NN denotes c
T2892 10750-10758 NN denotes turnover
T2895 10758-10759 . denotes .
T2896 10759-11087 sentence denotes In a fluorogenic in vitro assay, cathepsin D activity in total cellular extracts was not significantly altered in homozygous CbCln3Δex7/8 cells (376 ± 89 RFU/μg total protein), versus wild-type cells (324 ± 58 RFU/μg total protein), although a consistent trend towards increased enzymatic activity in mutant cells was observed.
T2897 10760-10762 IN denotes In
T2899 10763-10764 DT denotes a
T2901 10765-10776 JJ denotes fluorogenic
T2902 10777-10779 FW denotes in
T2903 10780-10785 FW denotes vitro
T2900 10786-10791 NN denotes assay
T2904 10791-10793 , denotes ,
T2905 10793-10802 NN denotes cathepsin
T2906 10803-10804 NN denotes D
T2907 10805-10813 NN denotes activity
T2908 10814-10816 IN denotes in
T2909 10817-10822 JJ denotes total
T2911 10823-10831 JJ denotes cellular
T2910 10832-10840 NNS denotes extracts
T2912 10841-10844 VBD denotes was
T2913 10845-10848 RB denotes not
T2914 10849-10862 RB denotes significantly
T2898 10863-10870 VBN denotes altered
T2915 10871-10873 IN denotes in
T2916 10874-10884 JJ denotes homozygous
T2918 10885-10895 NN denotes CbCln3Δex7
T2919 10895-10896 HYPH denotes /
T2920 10896-10897 CD denotes 8
T2917 10898-10903 NNS denotes cells
T2921 10904-10905 -LRB- denotes (
T2923 10905-10908 CD denotes 376
T2925 10909-10910 SYM denotes ±
T2924 10911-10913 CD denotes 89
T2922 10914-10917 NN denotes RFU
T2926 10917-10918 SYM denotes /
T2928 10918-10920 NN denotes μg
T2929 10921-10926 JJ denotes total
T2927 10927-10934 NN denotes protein
T2930 10934-10935 -RRB- denotes )
T2931 10935-10937 , denotes ,
T2932 10937-10943 CC denotes versus
T2933 10944-10948 JJ denotes wild
T2935 10948-10949 HYPH denotes -
T2934 10949-10953 NN denotes type
T2936 10954-10959 NNS denotes cells
T2937 10960-10961 -LRB- denotes (
T2939 10961-10964 CD denotes 324
T2941 10965-10966 IN denotes ±
T2940 10967-10969 CD denotes 58
T2938 10970-10973 NN denotes RFU
T2942 10973-10974 SYM denotes /
T2944 10974-10976 NN denotes μg
T2945 10977-10982 JJ denotes total
T2943 10983-10990 NN denotes protein
T2946 10990-10991 -RRB- denotes )
T2947 10991-10993 , denotes ,
T2948 10993-11001 IN denotes although
T2950 11002-11003 DT denotes a
T2952 11004-11014 JJ denotes consistent
T2951 11015-11020 NN denotes trend
T2953 11021-11028 IN denotes towards
T2954 11029-11038 VBN denotes increased
T2956 11039-11048 JJ denotes enzymatic
T2955 11049-11057 NN denotes activity
T2957 11058-11060 IN denotes in
T2958 11061-11067 NN denotes mutant
T2959 11068-11073 NNS denotes cells
T2960 11074-11077 VBD denotes was
T2949 11078-11086 VBN denotes observed
T2961 11086-11087 . denotes .
T2962 11087-11255 sentence denotes Thus, cathepsin D transport and processing are disrupted in homozygous CbCln3Δex7/8 cells in a manner such that enzymatic activity appears to be relatively unaffected.
T2963 11088-11092 RB denotes Thus
T2965 11092-11094 , denotes ,
T2966 11094-11103 NN denotes cathepsin
T2967 11104-11105 NN denotes D
T2968 11106-11115 NN denotes transport
T2969 11116-11119 CC denotes and
T2970 11120-11130 NN denotes processing
T2971 11131-11134 VBP denotes are
T2964 11135-11144 VBN denotes disrupted
T2972 11145-11147 IN denotes in
T2973 11148-11158 JJ denotes homozygous
T2975 11159-11169 NN denotes CbCln3Δex7
T2976 11169-11170 HYPH denotes /
T2977 11170-11171 CD denotes 8
T2974 11172-11177 NNS denotes cells
T2978 11178-11180 IN denotes in
T2979 11181-11182 DT denotes a
T2980 11183-11189 NN denotes manner
T2981 11190-11194 JJ denotes such
T2982 11195-11199 IN denotes that
T2984 11200-11209 JJ denotes enzymatic
T2985 11210-11218 NN denotes activity
T2983 11219-11226 VBZ denotes appears
T2986 11227-11229 TO denotes to
T2987 11230-11232 VB denotes be
T2988 11233-11243 RB denotes relatively
T2989 11244-11254 JJ denotes unaffected
T2990 11254-11255 . denotes .
T3349 11257-11267 JJ denotes Homozygous
T3351 11268-11278 NN denotes CbCln3Δex7
T3352 11278-11279 HYPH denotes /
T3353 11279-11280 CD denotes 8
T3350 11281-11286 NNS denotes cells
T3354 11287-11291 VBP denotes show
T3355 11292-11300 JJ denotes abnormal
T3357 11301-11309 NN denotes membrane
T3356 11310-11320 NNS denotes organelles
T3358 11320-11541 sentence denotes The abnormal transport and processing of cathepsin D suggested membrane trafficking disruptions in homozygous CbCln3Δex7/8 cells; therefore, we surveyed the subcellular distribution and morphology of membrane organelles.
T3359 11321-11324 DT denotes The
T3361 11325-11333 JJ denotes abnormal
T3360 11334-11343 NN denotes transport
T3363 11344-11347 CC denotes and
T3364 11348-11358 NN denotes processing
T3365 11359-11361 IN denotes of
T3366 11362-11371 NN denotes cathepsin
T3367 11372-11373 NN denotes D
T3362 11374-11383 VBD denotes suggested
T3369 11384-11392 NN denotes membrane
T3370 11393-11404 NN denotes trafficking
T3371 11405-11416 NNS denotes disruptions
T3372 11417-11419 IN denotes in
T3373 11420-11430 JJ denotes homozygous
T3375 11431-11441 NN denotes CbCln3Δex7
T3376 11441-11442 HYPH denotes /
T3377 11442-11443 CD denotes 8
T3374 11444-11449 NNS denotes cells
T3378 11449-11450 : denotes ;
T3379 11451-11460 RB denotes therefore
T3380 11460-11462 , denotes ,
T3381 11462-11464 PRP denotes we
T3368 11465-11473 VBD denotes surveyed
T3382 11474-11477 DT denotes the
T3384 11478-11489 JJ denotes subcellular
T3383 11490-11502 NN denotes distribution
T3385 11503-11506 CC denotes and
T3386 11507-11517 NN denotes morphology
T3387 11518-11520 IN denotes of
T3388 11521-11529 NN denotes membrane
T3389 11530-11540 NNS denotes organelles
T3390 11540-11541 . denotes .
T3391 11541-11783 sentence denotes Components of the secretory pathway, including the ER, cis-Golgi, and trans-Golgi, did not appear altered from wild-type appearance when labeled with the respective markers, protein disulfide isomerase (PDI), GM130, and VVL (data not shown).
T3392 11542-11552 NNS denotes Components
T3394 11553-11555 IN denotes of
T3395 11556-11559 DT denotes the
T3397 11560-11569 JJ denotes secretory
T3396 11570-11577 NN denotes pathway
T3398 11577-11579 , denotes ,
T3399 11579-11588 VBG denotes including
T3400 11589-11592 DT denotes the
T3401 11593-11595 NN denotes ER
T3402 11595-11597 , denotes ,
T3403 11597-11600 NN denotes cis
T3405 11600-11601 HYPH denotes -
T3404 11601-11606 NN denotes Golgi
T3406 11606-11608 , denotes ,
T3407 11608-11611 CC denotes and
T3408 11612-11617 NN denotes trans
T3410 11617-11618 HYPH denotes -
T3409 11618-11623 NN denotes Golgi
T3411 11623-11625 , denotes ,
T3412 11625-11628 VBD denotes did
T3413 11629-11632 RB denotes not
T3393 11633-11639 VB denotes appear
T3414 11640-11647 JJ denotes altered
T3415 11648-11652 IN denotes from
T3416 11653-11657 JJ denotes wild
T3418 11657-11658 HYPH denotes -
T3417 11658-11662 NN denotes type
T3419 11663-11673 NN denotes appearance
T3420 11674-11678 WRB denotes when
T3421 11679-11686 VBN denotes labeled
T3422 11687-11691 IN denotes with
T3423 11692-11695 DT denotes the
T3425 11696-11706 JJ denotes respective
T3424 11707-11714 NNS denotes markers
T3426 11714-11716 , denotes ,
T3427 11716-11723 NN denotes protein
T3429 11724-11733 NN denotes disulfide
T3428 11734-11743 NN denotes isomerase
T3430 11744-11745 -LRB- denotes (
T3431 11745-11748 NN denotes PDI
T3432 11748-11749 -RRB- denotes )
T3433 11749-11751 , denotes ,
T3434 11751-11756 NN denotes GM130
T3435 11756-11758 , denotes ,
T3436 11758-11761 CC denotes and
T3437 11762-11765 NN denotes VVL
T3438 11766-11767 -LRB- denotes (
T3440 11767-11771 NNS denotes data
T3441 11772-11775 RB denotes not
T3439 11776-11781 VBN denotes shown
T3442 11781-11782 -RRB- denotes )
T3443 11782-11783 . denotes .
T3444 11783-11933 sentence denotes By contrast, the lysosomal markers, Lysotracker and Lamp 2 had significantly altered signal in homozygous CbCln3Δex7/8 cells, versus wild-type cells.
T3445 11784-11786 IN denotes By
T3447 11787-11795 NN denotes contrast
T3448 11795-11797 , denotes ,
T3449 11797-11800 DT denotes the
T3451 11801-11810 JJ denotes lysosomal
T3450 11811-11818 NNS denotes markers
T3452 11818-11820 , denotes ,
T3453 11820-11831 NN denotes Lysotracker
T3454 11832-11835 CC denotes and
T3455 11836-11840 NN denotes Lamp
T3456 11841-11842 CD denotes 2
T3446 11843-11846 VBD denotes had
T3457 11847-11860 RB denotes significantly
T3458 11861-11868 JJ denotes altered
T3459 11869-11875 NN denotes signal
T3460 11876-11878 IN denotes in
T3461 11879-11889 JJ denotes homozygous
T3463 11890-11900 NN denotes CbCln3Δex7
T3464 11900-11901 HYPH denotes /
T3465 11901-11902 CD denotes 8
T3462 11903-11908 NNS denotes cells
T3466 11908-11910 , denotes ,
T3467 11910-11916 CC denotes versus
T3468 11917-11921 JJ denotes wild
T3470 11921-11922 HYPH denotes -
T3469 11922-11926 NN denotes type
T3471 11927-11932 NNS denotes cells
T3472 11932-11933 . denotes .
T3473 11933-12197 sentence denotes Wild-type cells exhibited brightly stained lysosomes that were large and clustered in the perinuclear region whereas homozygous CbCln3Δex7/8 lysosomes were lightly stained, smaller vesicles that were more diffusely scattered in the cytoplasm of the cell (Fig. 6).
T3474 11934-11938 JJ denotes Wild
T3476 11938-11939 HYPH denotes -
T3475 11939-11943 NN denotes type
T3477 11944-11949 NNS denotes cells
T3478 11950-11959 VBD denotes exhibited
T3479 11960-11968 RB denotes brightly
T3480 11969-11976 VBN denotes stained
T3481 11977-11986 NNS denotes lysosomes
T3482 11987-11991 WDT denotes that
T3483 11992-11996 VBD denotes were
T3484 11997-12002 JJ denotes large
T3485 12003-12006 CC denotes and
T3486 12007-12016 JJ denotes clustered
T3487 12017-12019 IN denotes in
T3488 12020-12023 DT denotes the
T3490 12024-12035 JJ denotes perinuclear
T3489 12036-12042 NN denotes region
T3491 12043-12050 IN denotes whereas
T3493 12051-12061 JJ denotes homozygous
T3495 12062-12072 NN denotes CbCln3Δex7
T3496 12072-12073 HYPH denotes /
T3497 12073-12074 CD denotes 8
T3494 12075-12084 NNS denotes lysosomes
T3492 12085-12089 VBD denotes were
T3498 12090-12097 RB denotes lightly
T3499 12098-12105 VBN denotes stained
T3501 12105-12107 , denotes ,
T3502 12107-12114 JJR denotes smaller
T3500 12115-12123 NNS denotes vesicles
T3503 12124-12128 WDT denotes that
T3505 12129-12133 VBD denotes were
T3506 12134-12138 RBR denotes more
T3507 12139-12148 RB denotes diffusely
T3504 12149-12158 VBN denotes scattered
T3508 12159-12161 IN denotes in
T3509 12162-12165 DT denotes the
T3510 12166-12175 NN denotes cytoplasm
T3511 12176-12178 IN denotes of
T3512 12179-12182 DT denotes the
T3513 12183-12187 NN denotes cell
T3514 12188-12189 -LRB- denotes (
T3515 12189-12193 NN denotes Fig.
T3516 12194-12195 CD denotes 6
T3517 12195-12196 -RRB- denotes )
T3518 12196-12197 . denotes .
T3519 12197-12265 sentence denotes Lamp 1 distribution was also altered, as previously noted (Fig. 3).
T3520 12198-12202 NN denotes Lamp
T3522 12203-12204 CD denotes 1
T3521 12205-12217 NN denotes distribution
T3524 12218-12221 VBD denotes was
T3525 12222-12226 RB denotes also
T3523 12227-12234 VBN denotes altered
T3526 12234-12236 , denotes ,
T3527 12236-12238 IN denotes as
T3529 12239-12249 RB denotes previously
T3528 12250-12255 VBN denotes noted
T3530 12256-12257 -LRB- denotes (
T3531 12257-12261 NN denotes Fig.
T3532 12262-12263 CD denotes 3
T3533 12263-12264 -RRB- denotes )
T3534 12264-12265 . denotes .
T3535 12265-12533 sentence denotes However, Lamp 1 and Lamp 2 total protein levels were similar in wild-type and homozygous CbCln3Δex7/8 cells by immunoblot analysis, indicating the altered signal likely reflects dispersed lysosomes or altered localization and/or epitope availability (data not shown).
T3536 12266-12273 RB denotes However
T3538 12273-12275 , denotes ,
T3539 12275-12279 NN denotes Lamp
T3541 12280-12281 CD denotes 1
T3542 12282-12285 CC denotes and
T3543 12286-12290 NN denotes Lamp
T3544 12291-12292 CD denotes 2
T3545 12293-12298 JJ denotes total
T3546 12299-12306 NN denotes protein
T3540 12307-12313 NNS denotes levels
T3537 12314-12318 VBD denotes were
T3547 12319-12326 JJ denotes similar
T3548 12327-12329 IN denotes in
T3549 12330-12334 JJ denotes wild
T3551 12334-12335 HYPH denotes -
T3550 12335-12339 NN denotes type
T3553 12340-12343 CC denotes and
T3554 12344-12354 JJ denotes homozygous
T3555 12355-12365 NN denotes CbCln3Δex7
T3556 12365-12366 HYPH denotes /
T3557 12366-12367 CD denotes 8
T3552 12368-12373 NNS denotes cells
T3558 12374-12376 IN denotes by
T3559 12377-12387 NN denotes immunoblot
T3560 12388-12396 NN denotes analysis
T3561 12396-12398 , denotes ,
T3562 12398-12408 VBG denotes indicating
T3563 12409-12412 DT denotes the
T3565 12413-12420 JJ denotes altered
T3564 12421-12427 NN denotes signal
T3567 12428-12434 RB denotes likely
T3566 12435-12443 VBZ denotes reflects
T3568 12444-12453 JJ denotes dispersed
T3569 12454-12463 NNS denotes lysosomes
T3570 12464-12466 CC denotes or
T3571 12467-12474 JJ denotes altered
T3572 12475-12487 NN denotes localization
T3573 12488-12491 CC denotes and
T3574 12491-12492 HYPH denotes /
T3575 12492-12494 CC denotes or
T3576 12495-12502 NN denotes epitope
T3577 12503-12515 NN denotes availability
T3578 12516-12517 -LRB- denotes (
T3580 12517-12521 NNS denotes data
T3581 12522-12525 RB denotes not
T3579 12526-12531 VBN denotes shown
T3582 12531-12532 -RRB- denotes )
T3583 12532-12533 . denotes .
T3584 12533-12685 sentence denotes It is noteworthy that Lysotracker dye, which selectively accumulates in acidic compartments, exhibited the most marked reduction in lysosomal labeling.
T3585 12534-12536 PRP denotes It
T3586 12537-12539 VBZ denotes is
T3587 12540-12550 JJ denotes noteworthy
T3588 12551-12555 IN denotes that
T3590 12556-12567 NN denotes Lysotracker
T3591 12568-12571 NN denotes dye
T3592 12571-12573 , denotes ,
T3593 12573-12578 WDT denotes which
T3595 12579-12590 RB denotes selectively
T3594 12591-12602 VBZ denotes accumulates
T3596 12603-12605 IN denotes in
T3597 12606-12612 JJ denotes acidic
T3598 12613-12625 NNS denotes compartments
T3599 12625-12627 , denotes ,
T3589 12627-12636 VBD denotes exhibited
T3600 12637-12640 DT denotes the
T3602 12641-12645 RBS denotes most
T3603 12646-12652 JJ denotes marked
T3601 12653-12662 NN denotes reduction
T3604 12663-12665 IN denotes in
T3605 12666-12675 JJ denotes lysosomal
T3606 12676-12684 NN denotes labeling
T3607 12684-12685 . denotes .
T3608 12685-12776 sentence denotes This observation may reflect altered lysosomal pH, an established finding in JNCL [10,15].
T3609 12686-12690 DT denotes This
T3610 12691-12702 NN denotes observation
T3612 12703-12706 MD denotes may
T3611 12707-12714 VB denotes reflect
T3613 12715-12722 JJ denotes altered
T3615 12723-12732 JJ denotes lysosomal
T3614 12733-12735 NN denotes pH
T3616 12735-12737 , denotes ,
T3617 12737-12739 DT denotes an
T3619 12740-12751 JJ denotes established
T3618 12752-12759 NN denotes finding
T3620 12760-12762 IN denotes in
T3621 12763-12767 NN denotes JNCL
T3622 12768-12769 -LRB- denotes [
T3624 12769-12771 CD denotes 10
T3625 12771-12772 , denotes ,
T3623 12772-12774 CD denotes 15
T3626 12774-12775 -RRB- denotes ]
T3627 12775-12776 . denotes .
T3628 12776-12777 sentence denotes
T9581 12787-12798 NN denotes Lysotracker
T9583 12799-12802 CC denotes and
T9584 12803-12807 NN denotes Lamp
T9585 12808-12809 CD denotes 2
T9582 12810-12818 NN denotes labeling
T9587 12819-12821 IN denotes of
T9588 12822-12826 JJ denotes wild
T9590 12826-12827 HYPH denotes -
T9589 12827-12831 NN denotes type
T9592 12832-12835 CC denotes and
T9593 12836-12846 JJ denotes homozygous
T9594 12847-12857 NN denotes CbCln3Δex7
T9595 12857-12858 HYPH denotes /
T9596 12858-12859 CD denotes 8
T9591 12860-12869 NNS denotes lysosomes
T9597 12870-12879 JJ denotes Lysosomal
T9598 12880-12888 NN denotes labeling
T9599 12889-12891 IN denotes of
T9600 12892-12896 JJ denotes wild
T9602 12896-12897 HYPH denotes -
T9601 12897-12901 NN denotes type
T9604 12902-12905 CC denotes and
T9605 12906-12916 JJ denotes homozygous
T9606 12917-12927 NN denotes CbCln3Δex7
T9607 12927-12928 HYPH denotes /
T9608 12928-12929 CD denotes 8
T9609 12930-12939 NN denotes precursor
T9603 12940-12945 NNS denotes cells
T9610 12946-12950 IN denotes with
T9611 12951-12962 NN denotes lysotracker
T9613 12963-12966 CC denotes and
T9614 12967-12971 NN denotes Lamp
T9615 12972-12973 CD denotes 2
T9612 12974-12982 NN denotes antibody
T9616 12983-12985 VBZ denotes is
T9586 12986-12991 VBN denotes shown
T9617 12991-12992 . denotes .
T9618 12992-13141 sentence denotes Lysotracker dye (top panels) labeled large, perinuclear-clustered lysosomes and scattered lysosomes in the periphery of wild-type cells (CbCln3+/+).
T9619 12993-13004 NN denotes Lysotracker
T9620 13005-13008 NN denotes dye
T9622 13009-13010 -LRB- denotes (
T9624 13010-13013 JJ denotes top
T9623 13014-13020 NNS denotes panels
T9625 13020-13021 -RRB- denotes )
T9621 13022-13029 VBN denotes labeled
T9626 13030-13035 JJ denotes large
T9628 13035-13037 , denotes ,
T9629 13037-13048 JJ denotes perinuclear
T9631 13048-13049 HYPH denotes -
T9630 13049-13058 VBN denotes clustered
T9627 13059-13068 NNS denotes lysosomes
T9632 13069-13072 CC denotes and
T9633 13073-13082 VBN denotes scattered
T9634 13083-13092 NNS denotes lysosomes
T9635 13093-13095 IN denotes in
T9636 13096-13099 DT denotes the
T9637 13100-13109 NN denotes periphery
T9638 13110-13112 IN denotes of
T9639 13113-13117 JJ denotes wild
T9641 13117-13118 HYPH denotes -
T9640 13118-13122 NN denotes type
T9642 13123-13128 NNS denotes cells
T9643 13129-13130 -LRB- denotes (
T9644 13130-13136 NN denotes CbCln3
T9645 13136-13137 SYM denotes +
T9646 13137-13138 HYPH denotes /
T9647 13138-13139 SYM denotes +
T9648 13139-13140 -RRB- denotes )
T9649 13140-13141 . denotes .
T9650 13141-13306 sentence denotes Lysotracker stain was dramatically reduced in homozygous mutant cells (CbCln3Δex7/8/Δex7/8), with smaller labeled vesicles and less apparent perinuclear clustering.
T9651 13142-13153 NN denotes Lysotracker
T9652 13154-13159 NN denotes stain
T9653 13160-13163 VBD denotes was
T9654 13164-13176 RB denotes dramatically
T9655 13177-13184 JJ denotes reduced
T9656 13185-13187 IN denotes in
T9657 13188-13198 JJ denotes homozygous
T9659 13199-13205 NN denotes mutant
T9658 13206-13211 NNS denotes cells
T9660 13212-13213 -LRB- denotes (
T9661 13213-13223 NN denotes CbCln3Δex7
T9662 13223-13224 HYPH denotes /
T9663 13224-13225 CD denotes 8
T9664 13225-13226 HYPH denotes /
T9665 13226-13230 NN denotes Δex7
T9666 13230-13231 HYPH denotes /
T9667 13231-13232 CD denotes 8
T9668 13232-13233 -RRB- denotes )
T9669 13233-13235 , denotes ,
T9670 13235-13239 IN denotes with
T9671 13240-13247 JJR denotes smaller
T9673 13248-13255 VBN denotes labeled
T9672 13256-13264 NNS denotes vesicles
T9674 13265-13268 CC denotes and
T9675 13269-13273 RBR denotes less
T9676 13274-13282 JJ denotes apparent
T9678 13283-13294 JJ denotes perinuclear
T9677 13295-13305 NN denotes clustering
T9679 13305-13306 . denotes .
T9680 13306-13536 sentence denotes Lamp 2 (bottom panels) immunostaining also showed reduced signal intensity with less perinuclear clustering in homozygous CbCln3Δex7/8 cells, although the effect was somewhat less dramatic than that observed with Lysotracker dye.
T9681 13307-13311 NN denotes Lamp
T9683 13312-13313 CD denotes 2
T9684 13314-13315 -LRB- denotes (
T9686 13315-13321 JJ denotes bottom
T9685 13322-13328 NNS denotes panels
T9687 13328-13329 -RRB- denotes )
T9682 13330-13344 NN denotes immunostaining
T9689 13345-13349 RB denotes also
T9688 13350-13356 VBD denotes showed
T9690 13357-13364 VBN denotes reduced
T9692 13365-13371 NN denotes signal
T9691 13372-13381 NN denotes intensity
T9693 13382-13386 IN denotes with
T9694 13387-13391 RBR denotes less
T9696 13392-13403 JJ denotes perinuclear
T9695 13404-13414 NN denotes clustering
T9697 13415-13417 IN denotes in
T9698 13418-13428 JJ denotes homozygous
T9700 13429-13439 NN denotes CbCln3Δex7
T9701 13439-13440 HYPH denotes /
T9702 13440-13441 CD denotes 8
T9699 13442-13447 NNS denotes cells
T9703 13447-13449 , denotes ,
T9704 13449-13457 IN denotes although
T9706 13458-13461 DT denotes the
T9707 13462-13468 NN denotes effect
T9705 13469-13472 VBD denotes was
T9708 13473-13481 RB denotes somewhat
T9709 13482-13486 RBR denotes less
T9710 13487-13495 JJ denotes dramatic
T9711 13496-13500 IN denotes than
T9712 13501-13505 DT denotes that
T9713 13506-13514 VBN denotes observed
T9714 13515-13519 IN denotes with
T9715 13520-13531 NN denotes Lysotracker
T9716 13532-13535 NN denotes dye
T9717 13535-13536 . denotes .
T9718 13536-13638 sentence denotes Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings.
T9719 13537-13541 JJ denotes Wild
T9721 13541-13542 HYPH denotes -
T9720 13542-13546 NN denotes type
T9723 13547-13550 CC denotes and
T9724 13551-13561 JJ denotes homozygous
T9725 13562-13572 NN denotes CbCln3Δex7
T9726 13572-13573 HYPH denotes /
T9727 13573-13574 CD denotes 8
T9728 13575-13583 JJ denotes confocal
T9722 13584-13590 NNS denotes images
T9730 13591-13595 VBD denotes were
T9729 13596-13604 VBN denotes captured
T9731 13605-13609 IN denotes with
T9732 13610-13619 JJ denotes identical
T9734 13620-13628 NN denotes exposure
T9733 13629-13637 NNS denotes settings
T9735 13637-13638 . denotes .
T9736 13638-13658 sentence denotes 60 × magnification.
T9737 13639-13641 CD denotes 60
T9739 13642-13643 SYM denotes ×
T9738 13644-13657 NN denotes magnification
T9740 13657-13658 . denotes .
T3632 13659-13660 NN denotes C
T3629 13659-13671 sentence denotes Consistent w
T3631 13660-13661 NNS denotes o
T3633 13661-13662 NN denotes n
T3634 13662-13663 JJ denotes s
T3635 13663-13664 IN denotes i
T3636 13664-13667 NN denotes ste
T3638 13667-13668 CC denotes n
T3637 13668-13669 NN denotes t
T3630 13670-13671 VBN denotes w
T3640 13671-13672 JJ denotes i
T3639 13671-13676 sentence denotes ith t
T3641 13672-13673 VBN denotes t
T3642 13673-13674 DT denotes h
T3643 13675-13676 NN denotes t
T3645 13676-13677 JJ denotes h
T3644 13676-13685 sentence denotes he altere
T3646 13677-13678 NNS denotes e
T3647 13679-13681 JJR denotes al
T3648 13681-13682 JJ denotes t
T3649 13682-13685 JJ denotes ere
T3651 13685-13686 VBD denotes d
T3650 13685-13697 sentence denotes d early endo
T3652 13687-13688 VBN denotes e
T3653 13688-13689 NN denotes a
T3654 13689-13691 JJ denotes rl
T3655 13691-13692 JJ denotes y
T3656 13693-13694 NN denotes e
T3657 13694-13695 IN denotes n
T3658 13695-13696 VBN denotes d
T3659 13696-13697 NN denotes o
T3662 13697-13698 JJ denotes s
T3660 13697-13702 sentence denotes some
T3663 13698-13699 JJ denotes o
T3661 13699-13701 NNS denotes me
T3664 13702-13704 sentence denotes ma
T3665 13702-13704 NN denotes ma
T3669 13704-13705 JJ denotes r
T3666 13704-13879 sentence denotes rker (EEA1) signal observed by immunostaining (Fig. 3), fluid-phase endocytosis was also altered in homozygous CbCln3Δex7/8 cells, as measured by dextran-FITC uptake (Fig. 7).
T3670 13705-13708 NN denotes ker
T3671 13709-13710 -LRB- denotes (
T3672 13710-13714 NN denotes EEA1
T3673 13714-13715 -RRB- denotes )
T3668 13716-13722 NN denotes signal
T3674 13723-13731 VBN denotes observed
T3675 13732-13734 IN denotes by
T3676 13735-13749 NN denotes immunostaining
T3677 13750-13751 -LRB- denotes (
T3678 13751-13755 NN denotes Fig.
T3679 13756-13757 CD denotes 3
T3680 13757-13758 -RRB- denotes )
T3681 13758-13760 , denotes ,
T3682 13760-13765 NN denotes fluid
T3684 13765-13766 HYPH denotes -
T3683 13766-13771 NN denotes phase
T3685 13772-13783 NN denotes endocytosis
T3686 13784-13787 VBD denotes was
T3687 13788-13792 RB denotes also
T3667 13793-13800 VBN denotes altered
T3688 13801-13803 IN denotes in
T3689 13804-13814 JJ denotes homozygous
T3691 13815-13825 NN denotes CbCln3Δex7
T3692 13825-13826 HYPH denotes /
T3693 13826-13827 CD denotes 8
T3690 13828-13833 NNS denotes cells
T3694 13833-13835 , denotes ,
T3695 13835-13837 IN denotes as
T3696 13838-13846 VBN denotes measured
T3697 13847-13849 IN denotes by
T3698 13850-13857 NN denotes dextran
T3700 13857-13858 HYPH denotes -
T3699 13858-13862 NN denotes FITC
T3701 13863-13869 NN denotes uptake
T3702 13870-13871 -LRB- denotes (
T3703 13871-13875 NN denotes Fig.
T3704 13876-13877 CD denotes 7
T3705 13877-13878 -RRB- denotes )
T3706 13878-13879 . denotes .
T3707 13879-14079 sentence denotes Following a 15-minute incubation in media containing dextran-FITC, wild-type and heterozygote cells displayed brightly stained, large endocytic vesicles that were clustered in the perinuclear region.
T3708 13880-13889 VBG denotes Following
T3710 13890-13891 DT denotes a
T3712 13892-13894 CD denotes 15
T3714 13894-13895 HYPH denotes -
T3713 13895-13901 NN denotes minute
T3711 13902-13912 NN denotes incubation
T3715 13913-13915 IN denotes in
T3716 13916-13921 NNS denotes media
T3717 13922-13932 VBG denotes containing
T3718 13933-13940 NN denotes dextran
T3720 13940-13941 HYPH denotes -
T3719 13941-13945 NN denotes FITC
T3721 13945-13947 , denotes ,
T3722 13947-13951 JJ denotes wild
T3724 13951-13952 HYPH denotes -
T3723 13952-13956 NN denotes type
T3726 13957-13960 CC denotes and
T3727 13961-13973 NN denotes heterozygote
T3725 13974-13979 NNS denotes cells
T3709 13980-13989 VBD denotes displayed
T3728 13990-13998 RB denotes brightly
T3729 13999-14006 VBN denotes stained
T3731 14006-14008 , denotes ,
T3732 14008-14013 JJ denotes large
T3733 14014-14023 JJ denotes endocytic
T3730 14024-14032 NNS denotes vesicles
T3734 14033-14037 WDT denotes that
T3736 14038-14042 VBD denotes were
T3735 14043-14052 VBN denotes clustered
T3737 14053-14055 IN denotes in
T3738 14056-14059 DT denotes the
T3740 14060-14071 JJ denotes perinuclear
T3739 14072-14078 NN denotes region
T3741 14078-14079 . denotes .
T3742 14079-14254 sentence denotes However, homozygous CbCln3Δex7/8 cells were less brightly stained with most dextran-FITC signal localizing to smaller vesicles scattered throughout the cytoplasm of the cell.
T3743 14080-14087 RB denotes However
T3745 14087-14089 , denotes ,
T3746 14089-14099 JJ denotes homozygous
T3748 14100-14110 NN denotes CbCln3Δex7
T3749 14110-14111 HYPH denotes /
T3750 14111-14112 CD denotes 8
T3747 14113-14118 NNS denotes cells
T3751 14119-14123 VBD denotes were
T3752 14124-14128 RBR denotes less
T3753 14129-14137 RB denotes brightly
T3744 14138-14145 VBN denotes stained
T3754 14146-14150 IN denotes with
T3755 14151-14155 JJS denotes most
T3757 14156-14163 NN denotes dextran
T3759 14163-14164 HYPH denotes -
T3758 14164-14168 NN denotes FITC
T3756 14169-14175 NN denotes signal
T3760 14176-14186 VBG denotes localizing
T3761 14187-14189 IN denotes to
T3762 14190-14197 JJR denotes smaller
T3763 14198-14206 NNS denotes vesicles
T3764 14207-14216 VBN denotes scattered
T3765 14217-14227 IN denotes throughout
T3766 14228-14231 DT denotes the
T3767 14232-14241 NN denotes cytoplasm
T3768 14242-14244 IN denotes of
T3769 14245-14248 DT denotes the
T3770 14249-14253 NN denotes cell
T3771 14253-14254 . denotes .
T3772 14254-14257 sentence denotes Fi
T3773 14255-14257 NN denotes Fi
T9816 14265-14276 NN denotes Endocytosis
T9818 14277-14279 IN denotes in
T9819 14280-14284 JJ denotes wild
T9821 14284-14285 HYPH denotes -
T9820 14285-14289 NN denotes type
T9823 14289-14291 , denotes ,
T9824 14291-14303 JJ denotes heterozygous
T9825 14304-14307 CC denotes and
T9826 14308-14318 JJ denotes homozygous
T9827 14319-14329 NN denotes CbCln3Δex7
T9828 14329-14330 HYPH denotes /
T9829 14330-14331 CD denotes 8
T9822 14332-14337 NNS denotes cells
T9830 14338-14345 NN denotes Dextran
T9832 14345-14346 HYPH denotes -
T9831 14346-14350 NN denotes FITC
T9833 14351-14357 NN denotes uptake
T9834 14358-14360 IN denotes in
T9835 14361-14365 JJ denotes wild
T9837 14365-14366 HYPH denotes -
T9836 14366-14370 NN denotes type
T9839 14370-14372 , denotes ,
T9840 14372-14384 JJ denotes heterozygous
T9841 14385-14388 CC denotes and
T9842 14389-14399 JJ denotes homozygous
T9843 14400-14410 NN denotes CbCln3Δex7
T9844 14410-14411 HYPH denotes /
T9845 14411-14412 CD denotes 8
T9846 14413-14422 NN denotes precursor
T9838 14423-14428 NNS denotes cells
T9847 14429-14431 VBZ denotes is
T9817 14432-14437 VBN denotes shown
T9848 14437-14438 . denotes .
T9849 14438-14670 sentence denotes In wild-type (CbCln3+/+, left panel) and heterozygous (CbCln3+/Δex7/8, middle panel) cells, dextran-FITC label was observed in a perinuclear-clustered vesicular pattern with scattered labeled vesicles also present in the periphery.
T9850 14439-14441 IN denotes In
T9852 14442-14446 JJ denotes wild
T9854 14446-14447 HYPH denotes -
T9853 14447-14451 NN denotes type
T9856 14452-14453 -LRB- denotes (
T9857 14453-14459 NN denotes CbCln3
T9858 14459-14460 SYM denotes +
T9859 14460-14461 HYPH denotes /
T9860 14461-14462 SYM denotes +
T9861 14462-14464 , denotes ,
T9863 14464-14468 JJ denotes left
T9862 14469-14474 NN denotes panel
T9864 14474-14475 -RRB- denotes )
T9865 14476-14479 CC denotes and
T9866 14480-14492 JJ denotes heterozygous
T9867 14493-14494 -LRB- denotes (
T9869 14494-14500 NN denotes CbCln3
T9871 14500-14501 SYM denotes +
T9872 14501-14502 HYPH denotes /
T9870 14502-14506 NN denotes Δex7
T9873 14506-14507 HYPH denotes /
T9874 14507-14508 CD denotes 8
T9875 14508-14510 , denotes ,
T9876 14510-14516 JJ denotes middle
T9868 14517-14522 NN denotes panel
T9877 14522-14523 -RRB- denotes )
T9855 14524-14529 NNS denotes cells
T9878 14529-14531 , denotes ,
T9879 14531-14538 NN denotes dextran
T9881 14538-14539 HYPH denotes -
T9880 14539-14543 NN denotes FITC
T9882 14544-14549 NN denotes label
T9883 14550-14553 VBD denotes was
T9851 14554-14562 VBN denotes observed
T9884 14563-14565 IN denotes in
T9885 14566-14567 DT denotes a
T9887 14568-14579 JJ denotes perinuclear
T9889 14579-14580 HYPH denotes -
T9888 14580-14589 VBN denotes clustered
T9890 14590-14599 JJ denotes vesicular
T9886 14600-14607 NN denotes pattern
T9891 14608-14612 IN denotes with
T9892 14613-14622 VBN denotes scattered
T9894 14623-14630 VBN denotes labeled
T9893 14631-14639 NNS denotes vesicles
T9895 14640-14644 RB denotes also
T9896 14645-14652 JJ denotes present
T9897 14653-14655 IN denotes in
T9898 14656-14659 DT denotes the
T9899 14660-14669 NN denotes periphery
T9900 14669-14670 . denotes .
T9901 14670-14857 sentence denotes In contrast, dextran-FITC label of homozygous mutant (CbCln3Δex7/8/Δex7/8, right panel) cells was reduced overall and exhibited smaller stained vesicles with less perinuclear clustering.
T9902 14671-14673 IN denotes In
T9904 14674-14682 NN denotes contrast
T9905 14682-14684 , denotes ,
T9906 14684-14691 NN denotes dextran
T9908 14691-14692 HYPH denotes -
T9907 14692-14696 NN denotes FITC
T9909 14697-14702 NN denotes label
T9910 14703-14705 IN denotes of
T9911 14706-14716 JJ denotes homozygous
T9912 14717-14723 NN denotes mutant
T9914 14724-14725 -LRB- denotes (
T9915 14725-14735 NN denotes CbCln3Δex7
T9917 14735-14736 HYPH denotes /
T9918 14736-14737 CD denotes 8
T9919 14737-14738 HYPH denotes /
T9916 14738-14742 NN denotes Δex7
T9920 14742-14743 HYPH denotes /
T9921 14743-14744 CD denotes 8
T9922 14744-14746 , denotes ,
T9924 14746-14751 JJ denotes right
T9923 14752-14757 NN denotes panel
T9925 14757-14758 -RRB- denotes )
T9913 14759-14764 NNS denotes cells
T9926 14765-14768 VBD denotes was
T9903 14769-14776 VBN denotes reduced
T9927 14777-14784 RB denotes overall
T9928 14785-14788 CC denotes and
T9929 14789-14798 VBD denotes exhibited
T9930 14799-14806 JJR denotes smaller
T9932 14807-14814 VBN denotes stained
T9931 14815-14823 NNS denotes vesicles
T9933 14824-14828 IN denotes with
T9934 14829-14833 RBR denotes less
T9936 14834-14845 JJ denotes perinuclear
T9935 14846-14856 NN denotes clustering
T9937 14856-14857 . denotes .
T9938 14857-14921 sentence denotes Confocal images were captured with identical exposure settings.
T9939 14858-14866 JJ denotes Confocal
T9940 14867-14873 NNS denotes images
T9942 14874-14878 VBD denotes were
T9941 14879-14887 VBN denotes captured
T9943 14888-14892 IN denotes with
T9944 14893-14902 JJ denotes identical
T9946 14903-14911 NN denotes exposure
T9945 14912-14920 NNS denotes settings
T9947 14920-14921 . denotes .
T9948 14921-14941 sentence denotes 40 × magnification.
T9949 14922-14924 CD denotes 40
T9951 14925-14926 SYM denotes ×
T9950 14927-14940 NN denotes magnification
T9952 14940-14941 . denotes .
T3775 14944-14945 NN denotes n
T3774 14944-14954 sentence denotes nally, bec
T3777 14945-14946 CC denotes a
T3778 14946-14947 NN denotes l
T3776 14947-14948 NNS denotes l
T3779 14948-14949 NN denotes y
T3780 14949-14951 , denotes ,
T3781 14951-14953 NN denotes be
T3782 14953-14954 NN denotes c
T3785 14954-14955 NN denotes a
T3783 14954-14962 sentence denotes ause sub
T3786 14955-14957 JJ denotes us
T3787 14957-14958 NN denotes e
T3788 14958-14959 , denotes
T3784 14959-14960 NNS denotes s
T3789 14960-14961 JJ denotes u
T3790 14961-14962 VBN denotes b
T3792 14962-14963 JJ denotes u
T3791 14962-14974 sentence denotes unit c is a
T3793 14963-14964 NN denotes n
T3795 14964-14966 JJ denotes it
T3794 14967-14968 NNS denotes c
T3796 14969-14970 VBD denotes i
T3797 14970-14971 JJR denotes s
T3798 14972-14973 VBN denotes a
T3800 14974-14975 NNS denotes m
T3799 14974-14978 sentence denotes mito
T3801 14975-14977 IN denotes it
T3802 14977-14978 NN denotes o
T3803 14978-14979 sentence denotes c
T3804 14978-14979 NN denotes c
T3808 14979-14987 JJ denotes hondrial
T3805 14979-15153 sentence denotes hondrial protein and its turnover proceeds through autophagic engulfment of mitochondria [13], we analyzed homozygous CbCln3Δex7/8 cell mitochondrial morphology and function.
T3807 14988-14995 NN denotes protein
T3809 14996-14999 CC denotes and
T3810 15000-15003 PRP$ denotes its
T3811 15004-15012 NN denotes turnover
T3812 15013-15021 VBZ denotes proceeds
T3813 15022-15029 IN denotes through
T3814 15030-15040 JJ denotes autophagic
T3815 15041-15051 NN denotes engulfment
T3816 15052-15054 IN denotes of
T3817 15055-15067 NNS denotes mitochondria
T3818 15068-15069 -LRB- denotes [
T3819 15069-15071 CD denotes 13
T3820 15071-15072 -RRB- denotes ]
T3821 15072-15074 , denotes ,
T3822 15074-15076 PRP denotes we
T3806 15077-15085 VBD denotes analyzed
T3823 15086-15096 JJ denotes homozygous
T3824 15097-15107 NN denotes CbCln3Δex7
T3825 15107-15108 HYPH denotes /
T3826 15108-15109 CD denotes 8
T3827 15110-15114 NN denotes cell
T3828 15115-15128 JJ denotes mitochondrial
T3829 15129-15139 NN denotes morphology
T3830 15140-15143 CC denotes and
T3831 15144-15152 NN denotes function
T3832 15152-15153 . denotes .
T3833 15153-15403 sentence denotes Mitochondrial distribution in homozygous CbCln3Δex7/8 cells was indistinguishable from wild-type and heterozygous cells; however, homozygous CbCln3Δex7/8 mitochondria appeared more elongated by grp75 marker immunostaining and TEM analysis (Fig. 8a).
T3834 15154-15167 JJ denotes Mitochondrial
T3835 15168-15180 NN denotes distribution
T3837 15181-15183 IN denotes in
T3838 15184-15194 JJ denotes homozygous
T3840 15195-15205 NN denotes CbCln3Δex7
T3841 15205-15206 HYPH denotes /
T3842 15206-15207 CD denotes 8
T3839 15208-15213 NNS denotes cells
T3836 15214-15217 VBD denotes was
T3844 15218-15235 JJ denotes indistinguishable
T3845 15236-15240 IN denotes from
T3846 15241-15245 JJ denotes wild
T3848 15245-15246 HYPH denotes -
T3847 15246-15250 NN denotes type
T3850 15251-15254 CC denotes and
T3851 15255-15267 JJ denotes heterozygous
T3849 15268-15273 NNS denotes cells
T3852 15273-15274 : denotes ;
T3853 15275-15282 RB denotes however
T3854 15282-15284 , denotes ,
T3855 15284-15294 JJ denotes homozygous
T3857 15295-15305 NN denotes CbCln3Δex7
T3858 15305-15306 HYPH denotes /
T3859 15306-15307 CD denotes 8
T3856 15308-15320 NNS denotes mitochondria
T3843 15321-15329 VBD denotes appeared
T3860 15330-15334 RBR denotes more
T3861 15335-15344 JJ denotes elongated
T3862 15345-15347 IN denotes by
T3863 15348-15353 NN denotes grp75
T3865 15354-15360 NN denotes marker
T3864 15361-15375 NN denotes immunostaining
T3866 15376-15379 CC denotes and
T3867 15380-15383 NN denotes TEM
T3868 15384-15392 NN denotes analysis
T3869 15393-15394 -LRB- denotes (
T3871 15394-15398 NN denotes Fig.
T3870 15399-15401 NN denotes 8a
T3872 15401-15402 -RRB- denotes )
T3873 15402-15403 . denotes .
T3874 15403-15597 sentence denotes 72% of homozygous mutant mitochondria were greater than 0.6 μm in length (range = 0.26 μm to 2.75 μm), while fewer wild-type mitochondria (51%) reached this length (range = 0.15 μm to 2.29 μm).
T3875 15404-15406 CD denotes 72
T3876 15406-15407 NN denotes %
T3878 15408-15410 IN denotes of
T3879 15411-15421 JJ denotes homozygous
T3881 15422-15428 NN denotes mutant
T3880 15429-15441 NNS denotes mitochondria
T3877 15442-15446 VBD denotes were
T3882 15447-15454 JJR denotes greater
T3884 15455-15459 IN denotes than
T3883 15460-15463 CD denotes 0.6
T3885 15464-15466 NN denotes μm
T3886 15467-15469 IN denotes in
T3887 15470-15476 NN denotes length
T3888 15477-15478 -LRB- denotes (
T3890 15478-15483 NN denotes range
T3891 15484-15485 SYM denotes =
T3892 15486-15490 CD denotes 0.26
T3889 15491-15493 NN denotes μm
T3893 15494-15496 IN denotes to
T3894 15497-15501 CD denotes 2.75
T3895 15502-15504 NN denotes μm
T3896 15504-15505 -RRB- denotes )
T3897 15505-15507 , denotes ,
T3898 15507-15512 IN denotes while
T3900 15513-15518 JJR denotes fewer
T3902 15519-15523 JJ denotes wild
T3904 15523-15524 HYPH denotes -
T3903 15524-15528 NN denotes type
T3901 15529-15541 NNS denotes mitochondria
T3905 15542-15543 -LRB- denotes (
T3907 15543-15545 CD denotes 51
T3906 15545-15546 NN denotes %
T3908 15546-15547 -RRB- denotes )
T3899 15548-15555 VBD denotes reached
T3909 15556-15560 DT denotes this
T3910 15561-15567 NN denotes length
T3911 15568-15569 -LRB- denotes (
T3913 15569-15574 NN denotes range
T3914 15575-15576 SYM denotes =
T3915 15577-15581 CD denotes 0.15
T3912 15582-15584 NN denotes μm
T3916 15585-15587 IN denotes to
T3917 15588-15592 CD denotes 2.29
T3918 15593-15595 NN denotes μm
T3919 15595-15596 -RRB- denotes )
T3920 15596-15597 . denotes .
T3921 15597-15684 sentence denotes Mitochondrial width was not altered in homozygous CbCln3Δex7/8 cells (data not shown).
T3922 15598-15611 JJ denotes Mitochondrial
T3923 15612-15617 NN denotes width
T3925 15618-15621 VBD denotes was
T3926 15622-15625 RB denotes not
T3924 15626-15633 VBN denotes altered
T3927 15634-15636 IN denotes in
T3928 15637-15647 JJ denotes homozygous
T3930 15648-15658 NN denotes CbCln3Δex7
T3931 15658-15659 HYPH denotes /
T3932 15659-15660 CD denotes 8
T3929 15661-15666 NNS denotes cells
T3933 15667-15668 -LRB- denotes (
T3935 15668-15672 NNS denotes data
T3936 15673-15676 RB denotes not
T3934 15677-15682 VBN denotes shown
T3937 15682-15683 -RRB- denotes )
T3938 15683-15684 . denotes .
T3939 15684-16017 sentence denotes Moreover, compared to wild-type or heterozygous cells, homozygous CbCln3Δex7/8 cells had significantly reduced cellular ATP levels (1.3 fold less, Fig. 8b) and exhibited reduced survival following hydrogen peroxide treatment (~50% of wild-type survival, Fig. 8c), suggesting impaired energy metabolism and oxidative stress response.
T3940 15685-15693 RB denotes Moreover
T3942 15693-15695 , denotes ,
T3943 15695-15703 VBN denotes compared
T3944 15704-15706 IN denotes to
T3945 15707-15711 JJ denotes wild
T3947 15711-15712 HYPH denotes -
T3946 15712-15716 NN denotes type
T3949 15717-15719 CC denotes or
T3950 15720-15732 JJ denotes heterozygous
T3948 15733-15738 NNS denotes cells
T3951 15738-15740 , denotes ,
T3952 15740-15750 JJ denotes homozygous
T3954 15751-15761 NN denotes CbCln3Δex7
T3955 15761-15762 HYPH denotes /
T3956 15762-15763 CD denotes 8
T3953 15764-15769 NNS denotes cells
T3941 15770-15773 VBD denotes had
T3957 15774-15787 RB denotes significantly
T3958 15788-15795 VBN denotes reduced
T3960 15796-15804 JJ denotes cellular
T3961 15805-15808 NN denotes ATP
T3959 15809-15815 NNS denotes levels
T3962 15816-15817 -LRB- denotes (
T3964 15817-15820 CD denotes 1.3
T3966 15821-15825 RB denotes fold
T3965 15826-15830 JJR denotes less
T3967 15830-15832 , denotes ,
T3963 15832-15836 NN denotes Fig.
T3968 15837-15839 CD denotes 8b
T3969 15839-15840 -RRB- denotes )
T3970 15841-15844 CC denotes and
T3971 15845-15854 VBD denotes exhibited
T3972 15855-15862 VBN denotes reduced
T3973 15863-15871 NN denotes survival
T3974 15872-15881 VBG denotes following
T3975 15882-15890 NN denotes hydrogen
T3976 15891-15899 NN denotes peroxide
T3977 15900-15909 NN denotes treatment
T3978 15910-15911 -LRB- denotes (
T3980 15911-15912 SYM denotes ~
T3981 15912-15914 CD denotes 50
T3982 15914-15915 NN denotes %
T3983 15916-15918 IN denotes of
T3984 15919-15923 JJ denotes wild
T3986 15923-15924 HYPH denotes -
T3985 15924-15928 NN denotes type
T3987 15929-15937 NN denotes survival
T3988 15937-15939 , denotes ,
T3989 15939-15943 NN denotes Fig.
T3979 15944-15946 NN denotes 8c
T3990 15946-15947 -RRB- denotes )
T3991 15947-15949 , denotes ,
T3992 15949-15959 VBG denotes suggesting
T3993 15960-15968 JJ denotes impaired
T3995 15969-15975 NN denotes energy
T3994 15976-15986 NN denotes metabolism
T3996 15987-15990 CC denotes and
T3997 15991-16000 JJ denotes oxidative
T3999 16001-16007 NN denotes stress
T3998 16008-16016 NN denotes response
T4000 16016-16017 . denotes .
T4001 16017-16118 sentence denotes Taken together, these data support impaired mitochondrial function in homozygous CbCln3Δex7/8 cells.
T4002 16018-16023 VBN denotes Taken
T4004 16024-16032 RB denotes together
T4005 16032-16034 , denotes ,
T4006 16034-16039 DT denotes these
T4007 16040-16044 NNS denotes data
T4003 16045-16052 VBP denotes support
T4008 16053-16061 JJ denotes impaired
T4010 16062-16075 JJ denotes mitochondrial
T4009 16076-16084 NN denotes function
T4011 16085-16087 IN denotes in
T4012 16088-16098 JJ denotes homozygous
T4014 16099-16109 NN denotes CbCln3Δex7
T4015 16109-16110 HYPH denotes /
T4016 16110-16111 CD denotes 8
T4013 16112-16117 NNS denotes cells
T4017 16117-16118 . denotes .
T10102 16129-16142 JJ denotes Mitochondrial
T10103 16143-16153 NN denotes morphology
T10104 16154-16157 CC denotes and
T10105 16158-16166 NN denotes function
T10106 16167-16169 IN denotes in
T10107 16170-16174 JJ denotes wild
T10109 16174-16175 HYPH denotes -
T10108 16175-16179 NN denotes type
T10111 16179-16181 , denotes ,
T10112 16181-16193 JJ denotes heterozygous
T10113 16194-16197 CC denotes and
T10114 16198-16208 JJ denotes homozygous
T10115 16209-16219 NN denotes CbCln3Δex7
T10116 16219-16220 HYPH denotes /
T10117 16220-16221 CD denotes 8
T10110 16222-16227 NNS denotes cells
T10118 16227-16336 sentence denotes a. Confocal and TEM micrographs of wild-type and homozygous CbCln3Δex7/8 mitochondrial morphology are shown.
T10119 16228-16229 LS denotes a
T10121 16229-16230 . denotes .
T10122 16231-16239 JJ denotes Confocal
T10124 16240-16243 CC denotes and
T10125 16244-16247 NN denotes TEM
T10123 16248-16259 NNS denotes micrographs
T10126 16260-16262 IN denotes of
T10127 16263-16267 JJ denotes wild
T10129 16267-16268 HYPH denotes -
T10128 16268-16272 NN denotes type
T10131 16273-16276 CC denotes and
T10132 16277-16287 JJ denotes homozygous
T10133 16288-16298 NN denotes CbCln3Δex7
T10134 16298-16299 HYPH denotes /
T10135 16299-16300 CD denotes 8
T10136 16301-16314 JJ denotes mitochondrial
T10130 16315-16325 NN denotes morphology
T10137 16326-16329 VBP denotes are
T10120 16330-16335 VBN denotes shown
T10138 16335-16336 . denotes .
T10139 16336-16572 sentence denotes Immunostaining with the inner mitochondrial membrane marker, grp75 (top panels) highlighted elongated mitochondria in homozygous mutant cells (CbCln3Δex7/8/Δex7/8), relative to wild-type mitochondria (CbCln3+/+) (insets, zoom = 2.75x).
T10140 16337-16351 NN denotes Immunostaining
T10142 16352-16356 IN denotes with
T10143 16357-16360 DT denotes the
T10145 16361-16366 JJ denotes inner
T10146 16367-16380 JJ denotes mitochondrial
T10147 16381-16389 NN denotes membrane
T10144 16390-16396 NN denotes marker
T10148 16396-16398 , denotes ,
T10149 16398-16403 NN denotes grp75
T10150 16404-16405 -LRB- denotes (
T10152 16405-16408 JJ denotes top
T10151 16409-16415 NNS denotes panels
T10153 16415-16416 -RRB- denotes )
T10141 16417-16428 VBD denotes highlighted
T10154 16429-16438 VBN denotes elongated
T10155 16439-16451 NNS denotes mitochondria
T10156 16452-16454 IN denotes in
T10157 16455-16465 JJ denotes homozygous
T10159 16466-16472 NN denotes mutant
T10158 16473-16478 NNS denotes cells
T10160 16479-16480 -LRB- denotes (
T10161 16480-16490 NN denotes CbCln3Δex7
T10163 16490-16491 HYPH denotes /
T10164 16491-16492 CD denotes 8
T10165 16492-16493 HYPH denotes /
T10162 16493-16497 NN denotes Δex7
T10166 16497-16498 HYPH denotes /
T10167 16498-16499 CD denotes 8
T10168 16499-16500 -RRB- denotes )
T10169 16500-16502 , denotes ,
T10170 16502-16510 JJ denotes relative
T10171 16511-16513 IN denotes to
T10172 16514-16518 JJ denotes wild
T10174 16518-16519 HYPH denotes -
T10173 16519-16523 NN denotes type
T10175 16524-16536 NNS denotes mitochondria
T10176 16537-16538 -LRB- denotes (
T10177 16538-16544 NN denotes CbCln3
T10178 16544-16545 SYM denotes +
T10179 16545-16546 HYPH denotes /
T10180 16546-16547 SYM denotes +
T10181 16547-16548 -RRB- denotes )
T10182 16549-16550 -LRB- denotes (
T10183 16550-16556 NNS denotes insets
T10184 16556-16558 , denotes ,
T10185 16558-16562 NN denotes zoom
T10187 16563-16564 SYM denotes =
T10186 16565-16569 CD denotes 2.75
T10188 16569-16570 SYM denotes x
T10189 16570-16571 -RRB- denotes )
T10190 16571-16572 . denotes .
T10191 16572-16643 sentence denotes Mitochondrial distribution was not altered from the wild-type pattern.
T10192 16573-16586 JJ denotes Mitochondrial
T10193 16587-16599 NN denotes distribution
T10195 16600-16603 VBD denotes was
T10196 16604-16607 RB denotes not
T10194 16608-16615 VBN denotes altered
T10197 16616-16620 IN denotes from
T10198 16621-16624 DT denotes the
T10200 16625-16629 JJ denotes wild
T10202 16629-16630 HYPH denotes -
T10201 16630-16634 NN denotes type
T10199 16635-16642 NN denotes pattern
T10203 16642-16643 . denotes .
T10204 16643-16726 sentence denotes Elongated homozygous CbCln3Δex7/8 mitochondria were also observed by TEM analysis.
T10205 16644-16653 VBN denotes Elongated
T10207 16654-16664 JJ denotes homozygous
T10208 16665-16675 NN denotes CbCln3Δex7
T10209 16675-16676 HYPH denotes /
T10210 16676-16677 CD denotes 8
T10206 16678-16690 NNS denotes mitochondria
T10212 16691-16695 VBD denotes were
T10213 16696-16700 RB denotes also
T10211 16701-16709 VBN denotes observed
T10214 16710-16712 IN denotes by
T10215 16713-16716 NN denotes TEM
T10216 16717-16725 NN denotes analysis
T10217 16725-16726 . denotes .
T10218 16726-16746 sentence denotes 60 × magnification.
T10219 16727-16729 CD denotes 60
T10221 16730-16731 SYM denotes ×
T10220 16732-16745 NN denotes magnification
T10222 16745-16746 . denotes .
T10223 16746-16851 sentence denotes b. Cellular ATP levels in wild-type, heterozygous and homozygous CbCln3Δex7/8 precursor cells are shown.
T10224 16747-16748 LS denotes b
T10226 16748-16749 . denotes .
T10227 16750-16758 JJ denotes Cellular
T10228 16759-16762 NN denotes ATP
T10229 16763-16769 NNS denotes levels
T10230 16770-16772 IN denotes in
T10231 16773-16777 JJ denotes wild
T10233 16777-16778 HYPH denotes -
T10232 16778-16782 NN denotes type
T10235 16782-16784 , denotes ,
T10236 16784-16796 JJ denotes heterozygous
T10237 16797-16800 CC denotes and
T10238 16801-16811 JJ denotes homozygous
T10239 16812-16822 NN denotes CbCln3Δex7
T10240 16822-16823 HYPH denotes /
T10241 16823-16824 CD denotes 8
T10242 16825-16834 NN denotes precursor
T10234 16835-16840 NNS denotes cells
T10243 16841-16844 VBP denotes are
T10225 16845-16850 VBN denotes shown
T10244 16850-16851 . denotes .
T10245 16851-17103 sentence denotes Wild-type (open bar) and heterozygous (gray bar) CbCln3Δex7/8 cells contained ~39 μM ATP, while homozygous CbCln3Δex7/8 cells (black bar) contained ~1.3 fold reduced levels of ATP (~30 μM), which was statistically significant in a t-test (p < 0.0001).
T10246 16852-16856 JJ denotes Wild
T10248 16856-16857 HYPH denotes -
T10247 16857-16861 NN denotes type
T10250 16862-16863 -LRB- denotes (
T10252 16863-16867 JJ denotes open
T10251 16868-16871 NN denotes bar
T10253 16871-16872 -RRB- denotes )
T10254 16873-16876 CC denotes and
T10255 16877-16889 JJ denotes heterozygous
T10256 16890-16891 -LRB- denotes (
T10258 16891-16895 JJ denotes gray
T10257 16896-16899 NN denotes bar
T10259 16899-16900 -RRB- denotes )
T10260 16901-16911 NN denotes CbCln3Δex7
T10261 16911-16912 HYPH denotes /
T10262 16912-16913 CD denotes 8
T10249 16914-16919 NNS denotes cells
T10263 16920-16929 VBD denotes contained
T10264 16930-16931 SYM denotes ~
T10265 16931-16933 CD denotes 39
T10266 16934-16936 NN denotes μM
T10267 16937-16940 NN denotes ATP
T10268 16940-16942 , denotes ,
T10269 16942-16947 IN denotes while
T10271 16948-16958 JJ denotes homozygous
T10273 16959-16969 NN denotes CbCln3Δex7
T10274 16969-16970 HYPH denotes /
T10275 16970-16971 CD denotes 8
T10272 16972-16977 NNS denotes cells
T10276 16978-16979 -LRB- denotes (
T10278 16979-16984 JJ denotes black
T10277 16985-16988 NN denotes bar
T10279 16988-16989 -RRB- denotes )
T10270 16990-16999 VBD denotes contained
T10280 17000-17001 SYM denotes ~
T10281 17001-17004 CD denotes 1.3
T10283 17005-17009 RB denotes fold
T10282 17010-17017 VBN denotes reduced
T10284 17018-17024 NNS denotes levels
T10285 17025-17027 IN denotes of
T10286 17028-17031 NN denotes ATP
T10287 17032-17033 -LRB- denotes (
T10288 17033-17034 SYM denotes ~
T10289 17034-17036 CD denotes 30
T10290 17037-17039 NN denotes μM
T10291 17039-17040 -RRB- denotes )
T10292 17040-17042 , denotes ,
T10293 17042-17047 WDT denotes which
T10294 17048-17051 VBD denotes was
T10295 17052-17065 RB denotes statistically
T10296 17066-17077 JJ denotes significant
T10297 17078-17080 IN denotes in
T10298 17081-17082 DT denotes a
T10300 17083-17084 NN denotes t
T10301 17084-17085 HYPH denotes -
T10299 17085-17089 NN denotes test
T10302 17090-17091 -LRB- denotes (
T10304 17091-17092 NN denotes p
T10305 17093-17094 SYM denotes <
T10303 17095-17101 CD denotes 0.0001
T10306 17101-17102 -RRB- denotes )
T10307 17102-17103 . denotes .
T10308 17103-17223 sentence denotes Wild-type and heterozygous CbCln3Δex7/8 cellular ATP levels were not statistically different from each other (p > 0.4).
T10309 17104-17108 JJ denotes Wild
T10311 17108-17109 HYPH denotes -
T10310 17109-17113 NN denotes type
T10313 17114-17117 CC denotes and
T10314 17118-17130 JJ denotes heterozygous
T10315 17131-17141 NN denotes CbCln3Δex7
T10317 17141-17142 HYPH denotes /
T10316 17142-17143 NN denotes 8
T10318 17144-17152 JJ denotes cellular
T10319 17153-17156 NN denotes ATP
T10312 17157-17163 NNS denotes levels
T10320 17164-17168 VBD denotes were
T10321 17169-17172 RB denotes not
T10322 17173-17186 RB denotes statistically
T10323 17187-17196 JJ denotes different
T10324 17197-17201 IN denotes from
T10325 17202-17206 DT denotes each
T10326 17207-17212 JJ denotes other
T10327 17213-17214 -LRB- denotes (
T10329 17214-17215 NN denotes p
T10330 17216-17217 SYM denotes >
T10328 17218-17221 CD denotes 0.4
T10331 17221-17222 -RRB- denotes )
T10332 17222-17223 . denotes .
T10333 17223-17303 sentence denotes A representative of triplicate experiments is shown (n = 6 in each experiment).
T10334 17224-17225 DT denotes A
T10335 17226-17240 NN denotes representative
T10337 17241-17243 IN denotes of
T10338 17244-17254 JJ denotes triplicate
T10339 17255-17266 NNS denotes experiments
T10340 17267-17269 VBZ denotes is
T10336 17270-17275 VBN denotes shown
T10341 17276-17277 -LRB- denotes (
T10343 17277-17278 NN denotes n
T10344 17279-17280 SYM denotes =
T10342 17281-17282 CD denotes 6
T10345 17283-17285 IN denotes in
T10346 17286-17290 DT denotes each
T10347 17291-17301 NN denotes experiment
T10348 17301-17302 -RRB- denotes )
T10349 17302-17303 . denotes .
T10350 17303-17376 sentence denotes c. Cell survival following 24-hour hydrogen peroxide treatment is shown.
T10351 17304-17305 LS denotes c
T10353 17305-17306 . denotes .
T10354 17307-17311 NN denotes Cell
T10355 17312-17320 NN denotes survival
T10356 17321-17330 VBG denotes following
T10357 17331-17333 CD denotes 24
T10359 17333-17334 HYPH denotes -
T10358 17334-17338 NN denotes hour
T10361 17339-17347 NN denotes hydrogen
T10362 17348-17356 NN denotes peroxide
T10360 17357-17366 NN denotes treatment
T10363 17367-17369 VBZ denotes is
T10352 17370-17375 VBN denotes shown
T10364 17375-17376 . denotes .
T10365 17376-17486 sentence denotes Homozygous CbCln3Δex7/8 cells were ~2-fold more sensitive to oxidative stress by hydrogen peroxide treatment.
T10366 17377-17387 JJ denotes Homozygous
T10368 17388-17398 NN denotes CbCln3Δex7
T10369 17398-17399 HYPH denotes /
T10370 17399-17400 CD denotes 8
T10367 17401-17406 NNS denotes cells
T10371 17407-17411 VBD denotes were
T10372 17412-17413 SYM denotes ~
T10373 17413-17419 RB denotes 2-fold
T10374 17420-17424 RBR denotes more
T10375 17425-17434 JJ denotes sensitive
T10376 17435-17437 IN denotes to
T10377 17438-17447 JJ denotes oxidative
T10378 17448-17454 NN denotes stress
T10379 17455-17457 IN denotes by
T10380 17458-17466 NN denotes hydrogen
T10381 17467-17475 NN denotes peroxide
T10382 17476-17485 NN denotes treatment
T10383 17485-17486 . denotes .
T10384 17486-17693 sentence denotes Wild-type (circle) and heterozygous (triangle) CbCln3Δex7/8 cells exhibited ~50% survival rates with 75–100 μM H2O2, whereas homozygous CbCln3Δex7/8 cells (squares) had a ~50% survival rate with 50 μM H2O2.
T10385 17487-17491 JJ denotes Wild
T10387 17491-17492 HYPH denotes -
T10386 17492-17496 NN denotes type
T10389 17497-17498 -LRB- denotes (
T10390 17498-17504 NN denotes circle
T10391 17504-17505 -RRB- denotes )
T10392 17506-17509 CC denotes and
T10393 17510-17522 JJ denotes heterozygous
T10394 17523-17524 -LRB- denotes (
T10395 17524-17532 NN denotes triangle
T10396 17532-17533 -RRB- denotes )
T10397 17534-17544 NN denotes CbCln3Δex7
T10398 17544-17545 HYPH denotes /
T10399 17545-17546 CD denotes 8
T10388 17547-17552 NNS denotes cells
T10400 17553-17562 VBD denotes exhibited
T10401 17563-17564 SYM denotes ~
T10402 17564-17566 CD denotes 50
T10403 17566-17567 NN denotes %
T10405 17568-17576 NN denotes survival
T10404 17577-17582 NNS denotes rates
T10406 17583-17587 IN denotes with
T10407 17588-17590 CD denotes 75
T10409 17590-17591 SYM denotes –
T10408 17591-17594 CD denotes 100
T10410 17595-17597 NN denotes μM
T10411 17598-17602 NN denotes H2O2
T10412 17602-17604 , denotes ,
T10413 17604-17611 IN denotes whereas
T10415 17612-17622 JJ denotes homozygous
T10417 17623-17633 NN denotes CbCln3Δex7
T10418 17633-17634 HYPH denotes /
T10419 17634-17635 CD denotes 8
T10416 17636-17641 NNS denotes cells
T10420 17642-17643 -LRB- denotes (
T10421 17643-17650 NNS denotes squares
T10422 17650-17651 -RRB- denotes )
T10414 17652-17655 VBD denotes had
T10423 17656-17657 DT denotes a
T10425 17658-17659 SYM denotes ~
T10426 17659-17661 CD denotes 50
T10427 17661-17662 NN denotes %
T10428 17663-17671 NN denotes survival
T10424 17672-17676 NN denotes rate
T10429 17677-17681 IN denotes with
T10430 17682-17684 CD denotes 50
T10431 17685-17687 NN denotes μM
T10432 17688-17692 NN denotes H2O2
T10433 17692-17693 . denotes .
T10434 17693-17773 sentence denotes A representative of triplicate experiments is shown (n = 4 in each experiment).
T10435 17694-17695 DT denotes A
T10436 17696-17710 NN denotes representative
T10438 17711-17713 IN denotes of
T10439 17714-17724 JJ denotes triplicate
T10440 17725-17736 NNS denotes experiments
T10441 17737-17739 VBZ denotes is
T10437 17740-17745 VBN denotes shown
T10442 17746-17747 -LRB- denotes (
T10444 17747-17748 NN denotes n
T10445 17749-17750 SYM denotes =
T10443 17751-17752 CD denotes 4
T10446 17753-17755 IN denotes in
T10447 17756-17760 DT denotes each
T10448 17761-17771 NN denotes experiment
T10449 17771-17772 -RRB- denotes )
T10450 17772-17773 . denotes .
R1000 T1871 T1872 compound Cln3,isoforms
R1001 T1872 T1866 nsubj isoforms,were
R1002 T1873 T1872 compound mRNA,isoforms
R1003 T1874 T1872 prep in,isoforms
R1004 T1875 T1876 amod wild,type
R1005 T1876 T1878 nmod type,cells
R1006 T1877 T1876 punct -,type
R1007 T1878 T1874 pobj cells,in
R1008 T1879 T1876 cc and,type
R1009 T1880 T1876 conj homozygous,type
R1010 T1881 T1866 acomp similar,were
R1011 T1882 T1881 prep to,similar
R1012 T1883 T1882 pobj those,to
R1013 T1884 T1883 acl observed,those
R1014 T1885 T1884 prep in,observed
R1015 T1886 T1887 amod total,RNA
R1016 T1887 T1885 pobj RNA,in
R1017 T1888 T1887 acl isolated,RNA
R1018 T1889 T1888 prep from,isolated
R1019 T1890 T1891 amod wild,type
R1020 T1891 T1893 nmod type,brain
R1021 T1892 T1891 punct -,type
R1022 T1893 T1889 pobj brain,from
R1023 T1894 T1891 cc or,type
R1024 T1895 T1891 conj homozygous,type
R1025 T1896 T1893 nmod Cln3Δex7,brain
R1026 T1897 T1896 punct /,Cln3Δex7
R1027 T1898 T1896 nummod 8,Cln3Δex7
R1028 T1899 T1893 amod knock,brain
R1029 T1900 T1899 punct -,knock
R1030 T1901 T1899 prt in,knock
R1031 T1902 T1866 punct ", ",were
R1032 T1903 T1866 advmod respectively,were
R1033 T1904 T1905 punct (,Fig.
R1034 T1905 T1866 parataxis Fig.,were
R1035 T1906 T1905 nummod 2,Fig.
R1036 T1907 T1905 punct ),Fig.
R1037 T1908 T1866 punct .,were
R1038 T1910 T1911 prep In,detected
R1039 T1912 T1910 pobj addition,In
R1040 T1913 T1911 punct ", ",detected
R1041 T1914 T1915 compound batp1,immunostaining
R1042 T1915 T1911 nsubj immunostaining,detected
R1043 T1916 T1917 compound mutant,product
R1044 T1917 T1911 dobj product,detected
R1045 T1918 T1917 compound battenin,product
R1046 T1919 T1917 prep in,product
R1047 T1920 T1921 amod homozygous,cells
R1048 T1921 T1919 pobj cells,in
R1049 T1922 T1921 nmod CbCln3Δex7,cells
R1050 T1923 T1922 punct /,CbCln3Δex7
R1051 T1924 T1922 nummod 8,CbCln3Δex7
R1052 T1925 T1917 punct ", ",product
R1053 T1926 T1917 prep in,product
R1054 T1927 T1928 det a,pattern
R1055 T1928 T1926 pobj pattern,in
R1056 T1929 T1928 amod similar,pattern
R1057 T1930 T1931 mark albeit,reduced
R1058 T1931 T1929 parataxis reduced,similar
R1059 T1932 T1928 amod cytoplasmic,pattern
R1060 T1933 T1928 punct ", ",pattern
R1061 T1934 T1928 amod vesicular,pattern
R1062 T1935 T1928 compound staining,pattern
R1063 T1936 T1928 prep as,pattern
R1064 T1937 T1936 pobj that,as
R1065 T1938 T1937 acl seen,that
R1066 T1939 T1938 prep in,seen
R1067 T1940 T1941 amod wild,type
R1068 T1941 T1943 compound type,cells
R1069 T1942 T1941 punct -,type
R1070 T1943 T1939 pobj cells,in
R1071 T1944 T1911 punct .,detected
R1072 T1946 T1947 compound Batp1,signal
R1073 T1947 T1948 nsubj signal,exhibited
R1074 T1949 T1950 det some,overlap
R1075 T1950 T1948 dobj overlap,exhibited
R1076 T1951 T1948 prep with,exhibited
R1077 T1952 T1953 det the,marker
R1078 T1953 T1951 pobj marker,with
R1079 T1954 T1953 amod lysosomal,marker
R1080 T1955 T1953 punct ", ",marker
R1081 T1956 T1953 appos Lamp1,marker
R1082 T1957 T1948 punct ", ",exhibited
R1083 T1958 T1948 cc but,exhibited
R1084 T1959 T1948 conj had,exhibited
R1085 T1960 T1961 advmod more,significant
R1086 T1961 T1962 amod significant,overlap
R1087 T1962 T1959 dobj overlap,had
R1088 T1963 T1959 prep with,had
R1089 T1964 T1965 amod early,antigen
R1090 T1965 T1963 pobj antigen,with
R1091 T1966 T1965 compound endosome,antigen
R1092 T1967 T1965 nummod 1,antigen
R1093 T1968 T1965 punct (,antigen
R1094 T1969 T1965 appos EEA1,antigen
R1095 T1970 T1965 punct ),antigen
R1096 T1971 T1965 cc and,antigen
R1097 T1972 T1973 det the,marker
R1098 T1973 T1965 conj marker,antigen
R1099 T1974 T1973 amod late,marker
R1100 T1975 T1973 amod endosomal,marker
R1101 T1976 T1973 punct ", ",marker
R1102 T1977 T1973 appos Rab7,marker
R1103 T1978 T1979 punct (,Fig.
R1104 T1979 T1959 parataxis Fig.,had
R1105 T1980 T1979 nummod 3,Fig.
R1106 T1981 T1979 punct ),Fig.
R1107 T1982 T1948 punct .,exhibited
R1108 T1984 T1985 advmod Only,overlap
R1109 T1985 T1987 nsubjpass overlap,observed
R1110 T1986 T1985 amod limited,overlap
R1111 T1988 T1987 auxpass was,observed
R1112 T1989 T1987 prep with,observed
R1113 T1990 T1991 amod recycling,endosomes
R1114 T1991 T1989 pobj endosomes,with
R1115 T1992 T1987 punct ", ",observed
R1116 T1993 T1994 mark as,determined
R1117 T1994 T1987 advcl determined,observed
R1118 T1995 T1994 agent by,determined
R1119 T1996 T1997 compound transferrin,receptor
R1120 T1997 T1998 compound receptor,co-staining
R1121 T1998 T1995 pobj co-staining,by
R1122 T1999 T2000 punct (,shown
R1123 T2000 T1987 parataxis shown,observed
R1124 T2001 T2000 nsubj data,shown
R1125 T2002 T2000 neg not,shown
R1126 T2003 T2000 punct ),shown
R1127 T2004 T1987 punct .,observed
R1128 T2006 T2007 advmod Intriguingly,altered
R1129 T2008 T2007 punct ", ",altered
R1130 T2009 T2010 nmod Lamp1,distribution
R1131 T2010 T2007 nsubjpass distribution,altered
R1132 T2011 T2009 cc and,Lamp1
R1133 T2012 T2009 conj EEA1,Lamp1
R1134 T2013 T2010 amod immunocytochemical,distribution
R1135 T2014 T2007 auxpass were,altered
R1136 T2015 T2007 prep in,altered
R1137 T2016 T2017 amod homozygous,cells
R1138 T2017 T2015 pobj cells,in
R1139 T2018 T2017 nmod CbCln3Δex7,cells
R1140 T2019 T2018 punct /,CbCln3Δex7
R1141 T2020 T2018 nummod 8,CbCln3Δex7
R1142 T2021 T2007 punct ", ",altered
R1143 T2022 T2007 prep with,altered
R1144 T2023 T2024 amod less,clustering
R1145 T2024 T2022 pobj clustering,with
R1146 T2025 T2024 amod perinuclear,clustering
R1147 T2026 T2024 prep than,clustering
R1148 T2027 T2026 prep in,than
R1149 T2028 T2029 amod wild,type
R1150 T2029 T2031 compound type,cells
R1151 T2030 T2029 punct -,type
R1152 T2031 T2027 pobj cells,in
R1153 T2032 T2007 punct ", ",altered
R1154 T2033 T2007 cc and,altered
R1155 T2034 T2035 compound Rab7,staining
R1156 T2035 T2036 nsubj staining,was
R1157 T2036 T2007 conj was,altered
R1158 T2037 T2036 advmod frequently,was
R1159 T2038 T2039 advmod less,intense
R1160 T2039 T2036 acomp intense,was
R1161 T2040 T2039 prep in,intense
R1162 T2041 T2042 amod homozygous,cells
R1163 T2042 T2040 pobj cells,in
R1164 T2043 T2042 nmod CbCln3Δex7,cells
R1165 T2044 T2043 punct /,CbCln3Δex7
R1166 T2045 T2043 nummod 8,CbCln3Δex7
R1167 T2046 T2047 punct (,Fig.
R1168 T2047 T2036 parataxis Fig.,was
R1169 T2048 T2047 nummod 3,Fig.
R1170 T2049 T2047 punct ),Fig.
R1171 T2050 T2036 punct .,was
R1172 T2052 T2053 amod Heterozygous,cells
R1173 T2053 T2057 nsubj cells,contained
R1174 T2054 T2053 nmod CbCln3Δex7,cells
R1175 T2055 T2054 punct /,CbCln3Δex7
R1176 T2056 T2054 nummod 8,CbCln3Δex7
R1177 T2058 T2059 det a,mixture
R1178 T2059 T2057 dobj mixture,contained
R1179 T2060 T2059 prep of,mixture
R1180 T2061 T2062 compound Cln3,products
R1181 T2062 T2060 pobj products,of
R1182 T2063 T2062 compound mRNA,products
R1183 T2064 T2059 prep from,mixture
R1184 T2065 T2066 preconj both,allele
R1185 T2066 T2064 pobj allele,from
R1186 T2067 T2066 det the,allele
R1187 T2068 T2069 amod wild,type
R1188 T2069 T2066 compound type,allele
R1189 T2070 T2069 punct -,type
R1190 T2071 T2066 cc and,allele
R1191 T2072 T2073 det the,allele
R1192 T2073 T2066 conj allele,allele
R1193 T2074 T2073 compound mutant,allele
R1194 T2075 T2057 punct ", ",contained
R1195 T2076 T2057 cc and,contained
R1196 T2077 T2078 compound batp1,signal
R1197 T2078 T2079 nsubj signal,was
R1198 T2079 T2057 conj was,contained
R1199 T2080 T2079 acomp similar,was
R1200 T2081 T2080 prep to,similar
R1201 T2082 T2081 pobj that,to
R1202 T2083 T2082 acl seen,that
R1203 T2084 T2083 prep in,seen
R1204 T2085 T2086 amod wild,type
R1205 T2086 T2088 compound type,cells
R1206 T2087 T2086 punct -,type
R1207 T2088 T2084 pobj cells,in
R1208 T2089 T2090 punct (,shown
R1209 T2090 T2079 parataxis shown,was
R1210 T2091 T2090 nsubj data,shown
R1211 T2092 T2090 neg not,shown
R1212 T2093 T2090 punct ),shown
R1213 T2094 T2079 punct .,was
R1214 T2096 T2097 prep During,displayed
R1215 T2098 T2099 amod sub-confluent,conditions
R1216 T2099 T2096 pobj conditions,During
R1217 T2100 T2099 compound growth,conditions
R1218 T2101 T2097 punct ", ",displayed
R1219 T2102 T2103 preconj neither,type
R1220 T2103 T2106 nmod type,cells
R1221 T2104 T2103 amod wild,type
R1222 T2105 T2103 punct -,type
R1223 T2106 T2097 nsubj cells,displayed
R1224 T2107 T2103 cc nor,type
R1225 T2108 T2109 amod homozygous,CbCln3Δex7
R1226 T2109 T2103 conj CbCln3Δex7,type
R1227 T2110 T2109 punct /,CbCln3Δex7
R1228 T2111 T2109 nummod 8,CbCln3Δex7
R1229 T2112 T2113 nmod autofluorescence,formation
R1230 T2113 T2097 dobj formation,displayed
R1231 T2114 T2112 cc or,autofluorescence
R1232 T2115 T2116 compound subunit,c
R1233 T2116 T2117 compound c,inclusion
R1234 T2117 T2112 conj inclusion,autofluorescence
R1235 T2118 T2119 punct (,shown
R1236 T2119 T2097 parataxis shown,displayed
R1237 T2120 T2119 nsubj data,shown
R1238 T2121 T2119 neg not,shown
R1239 T2122 T2119 punct ),shown
R1240 T2123 T2097 punct .,displayed
R1241 T2125 T2126 advmod However,elevated
R1242 T2127 T2126 punct ", ",elevated
R1243 T2128 T2129 advmod when,aged
R1244 T2129 T2126 advcl aged,elevated
R1245 T2130 T2129 nsubjpass cells,aged
R1246 T2131 T2129 auxpass were,aged
R1247 T2132 T2129 prep at,aged
R1248 T2133 T2132 pobj confluency,at
R1249 T2134 T2133 punct (,confluency
R1250 T2135 T2136 nummod 3,days
R1251 T2136 T2138 npadvmod days,post-confluency
R1252 T2137 T2135 punct +,3
R1253 T2138 T2133 advmod post-confluency,confluency
R1254 T2139 T2126 punct ),elevated
R1255 T2140 T2126 punct ", ",elevated
R1256 T2141 T2142 amod homozygous,levels
R1257 T2142 T2126 nsubjpass levels,elevated
R1258 T2143 T2142 nmod CbCln3Δex7,levels
R1259 T2144 T2143 punct /,CbCln3Δex7
R1260 T2145 T2143 nummod 8,CbCln3Δex7
R1261 T2146 T2142 amod cellular,levels
R1262 T2147 T2148 compound subunit,c
R1263 T2148 T2142 compound c,levels
R1264 T2149 T2126 auxpass were,elevated
R1265 T2150 T2126 prep beyond,elevated
R1266 T2151 T2152 amod normal,levels
R1267 T2152 T2150 pobj levels,beyond
R1268 T2153 T2154 amod wild,type
R1269 T2154 T2152 compound type,levels
R1270 T2155 T2154 punct -,type
R1271 T2156 T2126 prep by,elevated
R1272 T2157 T2156 pobj immunostaining,by
R1273 T2158 T2159 punct (,4a
R1274 T2159 T2157 parataxis 4a,immunostaining
R1275 T2160 T2159 compound Fig.,4a
R1276 T2161 T2159 punct ),4a
R1277 T2162 T2157 cc and,immunostaining
R1278 T2163 T2164 compound immunoblot,analysis
R1279 T2164 T2157 conj analysis,immunostaining
R1280 T2165 T2166 punct (,4b
R1281 T2166 T2164 parataxis 4b,analysis
R1282 T2167 T2166 compound Fig.,4b
R1283 T2168 T2166 punct ),4b
R1284 T2169 T2126 punct .,elevated
R1285 T2171 T2172 amod Autofluorescent,signal
R1286 T2172 T2173 nsubj signal,overlapped
R1287 T2174 T2173 advmod sometimes,overlapped
R1288 T2175 T2173 prep with,overlapped
R1289 T2176 T2177 compound subunit,c
R1290 T2177 T2178 compound c,signal
R1291 T2178 T2175 pobj signal,with
R1292 T2179 T2173 punct ", ",overlapped
R1293 T2180 T2173 cc but,overlapped
R1294 T2181 T2180 advmod also,but
R1295 T2182 T2173 conj was,overlapped
R1296 T2183 T2182 acomp elevated,was
R1297 T2184 T2185 advmod more,diffusely
R1298 T2185 T2182 advmod diffusely,was
R1299 T2186 T2187 punct (,4a
R1300 T2187 T2182 parataxis 4a,was
R1301 T2188 T2187 compound Fig.,4a
R1302 T2189 T2187 punct ),4a
R1303 T2190 T2173 punct .,overlapped
R1304 T2192 T2193 advmod Moreover,displayed
R1305 T2194 T2193 punct ", ",displayed
R1306 T2195 T2196 mark although,detected
R1307 T2196 T2193 advcl detected,displayed
R1308 T2197 T2198 amod multilamellar,profiles
R1309 T2198 T2196 nsubjpass profiles,detected
R1310 T2199 T2198 punct """",profiles
R1311 T2200 T2198 nmod fingerprint,profiles
R1312 T2201 T2198 punct """",profiles
R1313 T2202 T2196 auxpass were,detected
R1314 T2203 T2196 neg not,detected
R1315 T2204 T2193 punct ", ",displayed
R1316 T2205 T2206 amod confluency,aged
R1317 T2206 T2208 amod aged,cells
R1318 T2207 T2206 punct -,aged
R1319 T2208 T2193 nsubj cells,displayed
R1320 T2209 T2208 amod homozygous,cells
R1321 T2210 T2208 nmod CbCln3Δex7,cells
R1322 T2211 T2210 punct /,CbCln3Δex7
R1323 T2212 T2210 nummod 8,CbCln3Δex7
R1324 T2213 T2214 amod numerous,abnormalities
R1325 T2214 T2193 dobj abnormalities,displayed
R1326 T2215 T2214 amod ultrastructural,abnormalities
R1327 T2216 T2214 prep including,abnormalities
R1328 T2217 T2218 npadvmod electron,dense
R1329 T2218 T2219 amod dense,inclusions
R1330 T2219 T2216 pobj inclusions,including
R1331 T2220 T2219 amod characteristic,inclusions
R1332 T2221 T2220 prep of,characteristic
R1333 T2222 T2221 pobj lipofuscin,of
R1334 T2223 T2219 cc and,inclusions
R1335 T2224 T2225 amod large,autophagosomes
R1336 T2225 T2219 conj autophagosomes,inclusions
R1337 T2226 T2227 dep that,contained
R1338 T2227 T2225 relcl contained,autophagosomes
R1339 T2228 T2229 amod dense,structures
R1340 T2229 T2227 dobj structures,contained
R1341 T2230 T2229 compound core,structures
R1342 T2231 T2229 punct ", ",structures
R1343 T2232 T2233 amod degenerating,mitochondria
R1344 T2233 T2229 conj mitochondria,structures
R1345 T2234 T2233 punct ", ",mitochondria
R1346 T2235 T2233 cc and,mitochondria
R1347 T2236 T2237 amod many,vesicles
R1348 T2237 T2233 conj vesicles,mitochondria
R1349 T2238 T2237 amod smaller,vesicles
R1350 T2239 T2240 punct (,4c
R1351 T2240 T2227 parataxis 4c,contained
R1352 T2241 T2240 compound Fig.,4c
R1353 T2242 T2240 punct ),4c
R1354 T2243 T2193 punct .,displayed
R1355 T2245 T2246 compound Inclusion,bodies
R1356 T2246 T2247 nsubjpass bodies,observed
R1357 T2248 T2246 cc and,bodies
R1358 T2249 T2246 conj autophagosomes,bodies
R1359 T2250 T2247 auxpass were,observed
R1360 T2251 T2247 advmod infrequently,observed
R1361 T2252 T2247 prep in,observed
R1362 T2253 T2254 npadvmod confluency,aged
R1363 T2254 T2256 amod aged,cultures
R1364 T2255 T2254 punct -,aged
R1365 T2256 T2252 pobj cultures,in
R1366 T2257 T2258 amod wild,type
R1367 T2258 T2256 compound type,cultures
R1368 T2259 T2258 punct -,type
R1369 T2260 T2261 punct (,shown
R1370 T2261 T2247 parataxis shown,observed
R1371 T2262 T2261 nsubj data,shown
R1372 T2263 T2261 neg not,shown
R1373 T2264 T2261 punct ),shown
R1374 T2265 T2247 punct .,observed
R1375 T2526 T2527 amod Homozygous,cells
R1376 T2527 T2531 nsubj cells,process
R1377 T2528 T2527 nmod CbCln3Δex7,cells
R1378 T2529 T2528 punct /,CbCln3Δex7
R1379 T2530 T2528 nummod 8,CbCln3Δex7
R1380 T2532 T2527 cc and,cells
R1381 T2533 T2534 nmod Cln3Δex7,mice
R1382 T2534 T2527 conj mice,cells
R1383 T2535 T2533 punct /,Cln3Δex7
R1384 T2536 T2533 nummod 8,Cln3Δex7
R1385 T2537 T2534 amod knock,mice
R1386 T2538 T2537 punct -,knock
R1387 T2539 T2537 prt in,knock
R1388 T2540 T2541 compound cathepsin,D
R1389 T2541 T2531 dobj D,process
R1390 T2542 T2531 advmod abnormally,process
R1391 T2544 T2545 nsubj We,investigated
R1392 T2546 T2545 advmod next,investigated
R1393 T2547 T2548 det the,basis
R1394 T2548 T2545 dobj basis,investigated
R1395 T2549 T2548 prep for,basis
R1396 T2550 T2551 compound subunit,c
R1397 T2551 T2552 compound c,accumulation
R1398 T2552 T2549 pobj accumulation,for
R1399 T2553 T2545 punct ", ",investigated
R1400 T2554 T2545 advcl testing,investigated
R1401 T2555 T2556 det the,hypothesis
R1402 T2556 T2554 dobj hypothesis,testing
R1403 T2557 T2558 mark that,is
R1404 T2558 T2556 acl is,hypothesis
R1405 T2559 T2560 compound cathepsin,D
R1406 T2560 T2558 nsubj D,is
R1407 T2561 T2558 acomp abnormal,is
R1408 T2562 T2563 mark since,is
R1409 T2563 T2558 advcl is,is
R1410 T2564 T2563 nsubj it,is
R1411 T2565 T2563 acomp required,is
R1412 T2566 T2565 prep for,required
R1413 T2567 T2568 compound ATP,degradation
R1414 T2568 T2566 pobj degradation,for
R1415 T2569 T2568 compound synthase,degradation
R1416 T2570 T2571 compound subunit,c
R1417 T2571 T2568 compound c,degradation
R1418 T2572 T2568 prep in,degradation
R1419 T2573 T2574 det the,lysosome
R1420 T2574 T2572 pobj lysosome,in
R1421 T2575 T2576 punct [,13
R1422 T2576 T2554 parataxis 13,testing
R1423 T2577 T2576 punct ],13
R1424 T2578 T2545 punct .,investigated
R1425 T2580 T2581 nsubj We,tested
R1426 T2582 T2581 advmod first,tested
R1427 T2583 T2584 compound cathepsin,D
R1428 T2584 T2581 dobj D,tested
R1429 T2585 T2584 appos transport,D
R1430 T2586 T2585 cc and,transport
R1431 T2587 T2585 conj processing,transport
R1432 T2588 T2581 prep in,tested
R1433 T2589 T2590 amod homozygous,cells
R1434 T2590 T2588 pobj cells,in
R1435 T2591 T2590 nmod CbCln3Δex7,cells
R1436 T2592 T2591 punct /,CbCln3Δex7
R1437 T2593 T2591 nummod 8,CbCln3Δex7
R1438 T2594 T2590 cc and,cells
R1439 T2595 T2596 nmod Cln3Δex7,mice
R1440 T2596 T2590 conj mice,cells
R1441 T2597 T2595 punct /,Cln3Δex7
R1442 T2598 T2595 nummod 8,Cln3Δex7
R1443 T2599 T2581 advcl using,tested
R1444 T2600 T2601 amod anti-cathepsin,D
R1445 T2601 T2602 amod D,antibody
R1446 T2602 T2599 dobj antibody,using
R1447 T2603 T2604 dep that,recognizes
R1448 T2604 T2602 relcl recognizes,antibody
R1449 T2605 T2606 amod unprocessed,isoforms
R1450 T2606 T2604 dobj isoforms,recognizes
R1451 T2607 T2605 cc and,unprocessed
R1452 T2608 T2605 conj processed,unprocessed
R1453 T2609 T2610 compound cathepsin,D
R1454 T2610 T2606 compound D,isoforms
R1455 T2611 T2581 punct .,tested
R1456 T2613 T2614 nsubj Immunostaining,revealed
R1457 T2615 T2613 prep of,Immunostaining
R1458 T2616 T2617 amod wild,type
R1459 T2617 T2619 nmod type,cells
R1460 T2618 T2617 punct -,type
R1461 T2619 T2615 pobj cells,of
R1462 T2620 T2617 cc and,type
R1463 T2621 T2617 conj homozygous,type
R1464 T2622 T2619 nmod CbCln3Δex7,cells
R1465 T2623 T2622 punct /,CbCln3Δex7
R1466 T2624 T2622 nummod 8,CbCln3Δex7
R1467 T2625 T2626 det a,distribution
R1468 T2626 T2614 dobj distribution,revealed
R1469 T2627 T2626 amod perinuclear,distribution
R1470 T2628 T2627 cc and,perinuclear
R1471 T2629 T2627 conj punctate,perinuclear
R1472 T2630 T2626 amod vesicular,distribution
R1473 T2631 T2632 compound cathepsin,D
R1474 T2632 T2626 compound D,distribution
R1475 T2633 T2614 punct ", ",revealed
R1476 T2634 T2614 advcl consistent,revealed
R1477 T2635 T2634 prep with,consistent
R1478 T2636 T2637 poss its,transport
R1479 T2637 T2635 pobj transport,with
R1480 T2638 T2637 cc and,transport
R1481 T2639 T2637 conj processing,transport
R1482 T2640 T2637 prep through,transport
R1483 T2641 T2642 det the,pathway
R1484 T2642 T2640 pobj pathway,through
R1485 T2643 T2642 amod secretory,pathway
R1486 T2644 T2637 cc and,transport
R1487 T2645 T2637 conj delivery,transport
R1488 T2646 T2645 prep to,delivery
R1489 T2647 T2648 det the,lysosome
R1490 T2648 T2646 pobj lysosome,to
R1491 T2649 T2650 punct (,5a
R1492 T2650 T2614 parataxis 5a,revealed
R1493 T2651 T2650 compound Fig.,5a
R1494 T2652 T2650 punct ),5a
R1495 T2653 T2614 punct .,revealed
R1496 T2655 T2656 advmod However,was
R1497 T2657 T2656 punct ", ",was
R1498 T2658 T2656 prep in,was
R1499 T2659 T2660 amod homozygous,cells
R1500 T2660 T2658 pobj cells,in
R1501 T2661 T2660 nmod CbCln3Δex7,cells
R1502 T2662 T2661 punct /,CbCln3Δex7
R1503 T2663 T2661 nummod 8,CbCln3Δex7
R1504 T2664 T2656 punct ", ",was
R1505 T2665 T2666 compound cathepsin,D
R1506 T2666 T2667 compound D,distribution
R1507 T2667 T2656 nsubj distribution,was
R1508 T2668 T2669 advmod less,vesicular
R1509 T2669 T2656 acomp vesicular,was
R1510 T2670 T2669 cc and,vesicular
R1511 T2671 T2672 advmod more,clustered
R1512 T2672 T2669 conj clustered,vesicular
R1513 T2673 T2672 amod perinuclear,clustered
R1514 T2674 T2672 punct -,clustered
R1515 T2675 T2669 prep than,vesicular
R1516 T2676 T2675 prep in,than
R1517 T2677 T2678 amod wild,type
R1518 T2678 T2680 compound type,cells
R1519 T2679 T2678 punct -,type
R1520 T2680 T2676 pobj cells,in
R1521 T2681 T2656 punct .,was
R1522 T2683 T2684 nsubj Immunoblots,showed
R1523 T2685 T2683 prep of,Immunoblots
R1524 T2686 T2687 amod homozygous,extracts
R1525 T2687 T2685 pobj extracts,of
R1526 T2688 T2689 nmod CbCln3Δex7,cell
R1527 T2689 T2687 nmod cell,extracts
R1528 T2690 T2688 punct /,CbCln3Δex7
R1529 T2691 T2688 nummod 8,CbCln3Δex7
R1530 T2692 T2689 cc and,cell
R1531 T2693 T2694 nmod Cln3Δex7,tissue
R1532 T2694 T2689 conj tissue,cell
R1533 T2695 T2693 punct /,Cln3Δex7
R1534 T2696 T2693 nummod 8,Cln3Δex7
R1535 T2697 T2684 advmod also,showed
R1536 T2698 T2699 amod altered,levels
R1537 T2699 T2684 dobj levels,showed
R1538 T2700 T2699 amod relative,levels
R1539 T2701 T2699 prep of,levels
R1540 T2702 T2703 compound cathepsin,D
R1541 T2703 T2704 compound D,isoforms
R1542 T2704 T2701 pobj isoforms,of
R1543 T2705 T2706 punct (,5b
R1544 T2706 T2684 parataxis 5b,showed
R1545 T2707 T2706 compound Fig.,5b
R1546 T2708 T2706 punct ),5b
R1547 T2709 T2684 punct .,showed
R1548 T2711 T2712 compound Cathepsin,D
R1549 T2712 T2713 compound D,isoforms
R1550 T2713 T2714 nsubj isoforms,represent
R1551 T2715 T2713 punct ", ",isoforms
R1552 T2716 T2713 acl identified,isoforms
R1553 T2717 T2716 agent by,identified
R1554 T2718 T2719 amod relative,weights
R1555 T2719 T2717 pobj weights,by
R1556 T2720 T2719 amod molecular,weights
R1557 T2721 T2714 punct ", ",represent
R1558 T2722 T2723 det the,precursor
R1559 T2723 T2714 dobj precursor,represent
R1560 T2724 T2725 punct ~,45
R1561 T2725 T2726 nummod 45,kDa
R1562 T2726 T2723 compound kDa,precursor
R1563 T2727 T2723 punct ", ",precursor
R1564 T2728 T2729 det the,form
R1565 T2729 T2723 conj form,precursor
R1566 T2730 T2731 punct ~,43
R1567 T2731 T2732 nummod 43,kDa
R1568 T2732 T2729 nmod kDa,form
R1569 T2733 T2729 amod intermediate,form
R1570 T2734 T2735 amod single,chain
R1571 T2735 T2729 compound chain,form
R1572 T2736 T2729 prep of,form
R1573 T2737 T2738 det the,enzyme
R1574 T2738 T2736 pobj enzyme,of
R1575 T2739 T2729 punct ", ",form
R1576 T2740 T2729 cc and,form
R1577 T2741 T2742 det the,chain
R1578 T2742 T2729 conj chain,form
R1579 T2743 T2744 nummod 31,kDa
R1580 T2744 T2742 nmod kDa,chain
R1581 T2745 T2742 amod heavy,chain
R1582 T2746 T2742 prep of,chain
R1583 T2747 T2748 det the,form
R1584 T2748 T2746 pobj form,of
R1585 T2749 T2750 amod double,chain
R1586 T2750 T2748 compound chain,form
R1587 T2751 T2750 punct -,chain
R1588 T2752 T2748 prep of,form
R1589 T2753 T2754 det the,enzyme
R1590 T2754 T2752 pobj enzyme,of
R1591 T2755 T2754 amod mature,enzyme
R1592 T2756 T2757 punct [,14
R1593 T2757 T2714 parataxis 14,represent
R1594 T2758 T2757 punct ],14
R1595 T2759 T2714 punct .,represent
R1596 T2761 T2762 prep In,reduced
R1597 T2763 T2764 amod homozygous,extracts
R1598 T2764 T2761 pobj extracts,In
R1599 T2765 T2766 nmod CbCln3Δex7,cell
R1600 T2766 T2764 nmod cell,extracts
R1601 T2767 T2765 punct /,CbCln3Δex7
R1602 T2768 T2765 nummod 8,CbCln3Δex7
R1603 T2769 T2766 cc and,cell
R1604 T2770 T2771 nmod Cln3Δex7,tissue
R1605 T2771 T2766 conj tissue,cell
R1606 T2772 T2770 punct /,Cln3Δex7
R1607 T2773 T2770 nummod 8,Cln3Δex7
R1608 T2774 T2762 punct ", ",reduced
R1609 T2775 T2776 det the,precursor
R1610 T2776 T2762 nsubjpass precursor,reduced
R1611 T2777 T2776 cc and,precursor
R1612 T2778 T2779 amod heavy,chains
R1613 T2779 T2776 conj chains,precursor
R1614 T2780 T2762 auxpass were,reduced
R1615 T2781 T2762 punct ", ",reduced
R1616 T2782 T2762 cc and,reduced
R1617 T2783 T2784 det the,chain
R1618 T2784 T2786 nsubj chain,was
R1619 T2785 T2784 amod single,chain
R1620 T2786 T2762 conj was,reduced
R1621 T2787 T2788 advmod slightly,elevated
R1622 T2788 T2786 acomp elevated,was
R1623 T2789 T2786 prep compared,was
R1624 T2790 T2789 prep to,compared
R1625 T2791 T2792 amod wild,type
R1626 T2792 T2794 compound type,extracts
R1627 T2793 T2792 punct -,type
R1628 T2794 T2790 pobj extracts,to
R1629 T2795 T2786 punct .,was
R1630 T2797 T2798 det The,media
R1631 T2798 T2801 nsubj media,contain
R1632 T2799 T2800 amod cellular,growth
R1633 T2800 T2798 compound growth,media
R1634 T2802 T2801 aux did,contain
R1635 T2803 T2801 neg not,contain
R1636 T2804 T2805 amod altered,levels
R1637 T2805 T2801 dobj levels,contain
R1638 T2806 T2805 prep of,levels
R1639 T2807 T2808 compound cathepsin,D
R1640 T2808 T2806 pobj D,of
R1641 T2809 T2801 punct ", ",contain
R1642 T2810 T2801 advcl indicating,contain
R1643 T2811 T2812 compound enzyme,secretion
R1644 T2812 T2813 nsubjpass secretion,affected
R1645 T2813 T2810 advcl affected,indicating
R1646 T2814 T2813 auxpass was,affected
R1647 T2815 T2813 neg not,affected
R1648 T2816 T2801 punct .,contain
R1649 T2818 T2819 amod Heterozygous,mice
R1650 T2819 T2823 nsubj mice,were
R1651 T2820 T2819 nmod Cln3Δex7,mice
R1652 T2821 T2820 punct /,Cln3Δex7
R1653 T2822 T2820 nummod 8,Cln3Δex7
R1654 T2824 T2819 cc and,mice
R1655 T2825 T2826 nmod CbCln3Δex7,cells
R1656 T2826 T2819 conj cells,mice
R1657 T2827 T2825 punct /,CbCln3Δex7
R1658 T2828 T2825 nummod 8,CbCln3Δex7
R1659 T2829 T2823 acomp indistinguishable,were
R1660 T2830 T2829 prep from,indistinguishable
R1661 T2831 T2832 amod wild,type
R1662 T2832 T2830 pobj type,from
R1663 T2833 T2832 punct -,type
R1664 T2834 T2823 punct ", ",were
R1665 T2835 T2836 mark as,expected
R1666 T2836 T2823 advcl expected,were
R1667 T2837 T2836 prep for,expected
R1668 T2838 T2839 det a,phenotype
R1669 T2839 T2837 pobj phenotype,for
R1670 T2840 T2839 amod recessive,phenotype
R1671 T2841 T2839 compound disease,phenotype
R1672 T2842 T2843 punct (,shown
R1673 T2843 T2836 parataxis shown,expected
R1674 T2844 T2843 nsubj data,shown
R1675 T2845 T2843 neg not,shown
R1676 T2846 T2843 punct ),shown
R1677 T2847 T2823 punct .,were
R1678 T2855 T2856 amod c,t
R1679 T2862 T2863 amod e,a
R1680 T2865 T2866 advmod l,ed
R1681 T2867 T2866 nsubj er,ed
R1682 T2868 T2867 nmod t,er
R1683 T2873 T2874 nsubjpass p,tested
R1684 T2876 T2877 compound sin,D
R1685 T2877 T2875 compound D,processing
R1686 T2878 T2873 prep on,p
R1687 T2879 T2880 amod enzymatic,activity
R1688 T2880 T2878 pobj activity,on
R1689 T2881 T2874 auxpass was,tested
R1690 T2882 T2874 advmod next,tested
R1691 T2883 T2884 aux to,determine
R1692 T2884 T2874 advcl determine,tested
R1693 T2885 T2886 mark if,accounts
R1694 T2886 T2884 ccomp accounts,determine
R1695 T2887 T2888 amod altered,activity
R1696 T2888 T2886 nsubj activity,accounts
R1697 T2889 T2888 amod enzymatic,activity
R1698 T2890 T2886 prep for,accounts
R1699 T2891 T2892 amod inefficient,turnover
R1700 T2892 T2890 pobj turnover,for
R1701 T2893 T2894 compound subunit,c
R1702 T2894 T2892 compound c,turnover
R1703 T2895 T2874 punct .,tested
R1704 T2897 T2898 prep In,altered
R1705 T2899 T2900 det a,assay
R1706 T2900 T2897 pobj assay,In
R1707 T2901 T2900 amod fluorogenic,assay
R1708 T2902 T2903 advmod in,vitro
R1709 T2903 T2900 amod vitro,assay
R1710 T2904 T2898 punct ", ",altered
R1711 T2905 T2906 compound cathepsin,D
R1712 T2906 T2907 compound D,activity
R1713 T2907 T2898 nsubjpass activity,altered
R1714 T2908 T2907 prep in,activity
R1715 T2909 T2910 amod total,extracts
R1716 T2910 T2908 pobj extracts,in
R1717 T2911 T2910 amod cellular,extracts
R1718 T2912 T2898 auxpass was,altered
R1719 T2913 T2898 neg not,altered
R1720 T2914 T2898 advmod significantly,altered
R1721 T2915 T2898 prep in,altered
R1722 T2916 T2917 amod homozygous,cells
R1723 T2917 T2915 pobj cells,in
R1724 T2918 T2917 nmod CbCln3Δex7,cells
R1725 T2919 T2918 punct /,CbCln3Δex7
R1726 T2920 T2918 nummod 8,CbCln3Δex7
R1727 T2921 T2922 punct (,RFU
R1728 T2922 T2917 parataxis RFU,cells
R1729 T2923 T2924 quantmod 376,89
R1730 T2924 T2922 nummod 89,RFU
R1731 T2925 T2924 punct ±,89
R1732 T2926 T2927 punct /,protein
R1733 T2927 T2922 prep protein,RFU
R1734 T2928 T2927 nmod μg,protein
R1735 T2929 T2927 amod total,protein
R1736 T2930 T2922 punct ),RFU
R1737 T2931 T2917 punct ", ",cells
R1738 T2932 T2917 cc versus,cells
R1739 T2933 T2934 amod wild,type
R1740 T2934 T2936 compound type,cells
R1741 T2935 T2934 punct -,type
R1742 T2936 T2917 conj cells,cells
R1743 T2937 T2938 punct (,RFU
R1744 T2938 T2936 parataxis RFU,cells
R1745 T2939 T2940 quantmod 324,58
R1746 T2940 T2938 nummod 58,RFU
R1747 T2941 T2940 quantmod ±,58
R1748 T2942 T2943 punct /,protein
R1749 T2943 T2938 prep protein,RFU
R1750 T2944 T2943 nmod μg,protein
R1751 T2945 T2943 amod total,protein
R1752 T2946 T2938 punct ),RFU
R1753 T2947 T2898 punct ", ",altered
R1754 T2948 T2949 mark although,observed
R1755 T2949 T2898 advcl observed,altered
R1756 T2950 T2951 det a,trend
R1757 T2951 T2949 nsubjpass trend,observed
R1758 T2952 T2951 amod consistent,trend
R1759 T2953 T2951 prep towards,trend
R1760 T2954 T2955 amod increased,activity
R1761 T2955 T2953 pobj activity,towards
R1762 T2956 T2955 amod enzymatic,activity
R1763 T2957 T2955 prep in,activity
R1764 T2958 T2959 compound mutant,cells
R1765 T2959 T2957 pobj cells,in
R1766 T2960 T2949 auxpass was,observed
R1767 T2961 T2898 punct .,altered
R1768 T2963 T2964 advmod Thus,disrupted
R1769 T2965 T2964 punct ", ",disrupted
R1770 T2966 T2967 compound cathepsin,D
R1771 T2967 T2964 nsubjpass D,disrupted
R1772 T2968 T2967 appos transport,D
R1773 T2969 T2968 cc and,transport
R1774 T2970 T2968 conj processing,transport
R1775 T2971 T2964 auxpass are,disrupted
R1776 T2972 T2964 prep in,disrupted
R1777 T2973 T2974 amod homozygous,cells
R1778 T2974 T2972 pobj cells,in
R1779 T2975 T2974 nmod CbCln3Δex7,cells
R1780 T2976 T2975 punct /,CbCln3Δex7
R1781 T2977 T2975 nummod 8,CbCln3Δex7
R1782 T2978 T2964 prep in,disrupted
R1783 T2979 T2980 det a,manner
R1784 T2980 T2978 pobj manner,in
R1785 T2981 T2980 amod such,manner
R1786 T2982 T2983 mark that,appears
R1787 T2983 T2981 ccomp appears,such
R1788 T2984 T2985 amod enzymatic,activity
R1789 T2985 T2983 nsubj activity,appears
R1790 T2986 T2987 aux to,be
R1791 T2987 T2983 xcomp be,appears
R1792 T2988 T2989 advmod relatively,unaffected
R1793 T2989 T2987 acomp unaffected,be
R1794 T2990 T2964 punct .,disrupted
R1795 T3349 T3350 amod Homozygous,cells
R1796 T3350 T3354 nsubj cells,show
R1797 T3351 T3350 nmod CbCln3Δex7,cells
R1798 T3352 T3351 punct /,CbCln3Δex7
R1799 T3353 T3351 nummod 8,CbCln3Δex7
R1800 T3355 T3356 amod abnormal,organelles
R1801 T3356 T3354 dobj organelles,show
R1802 T3357 T3356 compound membrane,organelles
R1803 T3359 T3360 det The,transport
R1804 T3360 T3362 nsubj transport,suggested
R1805 T3361 T3360 amod abnormal,transport
R1806 T3362 T3368 ccomp suggested,surveyed
R1807 T3363 T3360 cc and,transport
R1808 T3364 T3360 conj processing,transport
R1809 T3365 T3360 prep of,transport
R1810 T3366 T3367 compound cathepsin,D
R1811 T3367 T3365 pobj D,of
R1812 T3369 T3370 compound membrane,trafficking
R1813 T3370 T3371 compound trafficking,disruptions
R1814 T3371 T3362 dobj disruptions,suggested
R1815 T3372 T3362 prep in,suggested
R1816 T3373 T3374 amod homozygous,cells
R1817 T3374 T3372 pobj cells,in
R1818 T3375 T3374 nmod CbCln3Δex7,cells
R1819 T3376 T3375 punct /,CbCln3Δex7
R1820 T3377 T3375 nummod 8,CbCln3Δex7
R1821 T3378 T3368 punct ;,surveyed
R1822 T3379 T3368 advmod therefore,surveyed
R1823 T3380 T3368 punct ", ",surveyed
R1824 T3381 T3368 nsubj we,surveyed
R1825 T3382 T3383 det the,distribution
R1826 T3383 T3368 dobj distribution,surveyed
R1827 T3384 T3383 amod subcellular,distribution
R1828 T3385 T3383 cc and,distribution
R1829 T3386 T3383 conj morphology,distribution
R1830 T3387 T3383 prep of,distribution
R1831 T3388 T3389 compound membrane,organelles
R1832 T3389 T3387 pobj organelles,of
R1833 T3390 T3368 punct .,surveyed
R1834 T3392 T3393 nsubj Components,appear
R1835 T3394 T3392 prep of,Components
R1836 T3395 T3396 det the,pathway
R1837 T3396 T3394 pobj pathway,of
R1838 T3397 T3396 amod secretory,pathway
R1839 T3398 T3392 punct ", ",Components
R1840 T3399 T3392 prep including,Components
R1841 T3400 T3401 det the,ER
R1842 T3401 T3399 pobj ER,including
R1843 T3402 T3401 punct ", ",ER
R1844 T3403 T3404 compound cis,Golgi
R1845 T3404 T3401 conj Golgi,ER
R1846 T3405 T3404 punct -,Golgi
R1847 T3406 T3404 punct ", ",Golgi
R1848 T3407 T3404 cc and,Golgi
R1849 T3408 T3409 compound trans,Golgi
R1850 T3409 T3404 conj Golgi,Golgi
R1851 T3410 T3409 punct -,Golgi
R1852 T3411 T3393 punct ", ",appear
R1853 T3412 T3393 aux did,appear
R1854 T3413 T3393 neg not,appear
R1855 T3414 T3393 oprd altered,appear
R1856 T3415 T3414 prep from,altered
R1857 T3416 T3417 amod wild,type
R1858 T3417 T3419 compound type,appearance
R1859 T3418 T3417 punct -,type
R1860 T3419 T3415 pobj appearance,from
R1861 T3420 T3421 advmod when,labeled
R1862 T3421 T3393 advcl labeled,appear
R1863 T3422 T3421 prep with,labeled
R1864 T3423 T3424 det the,markers
R1865 T3424 T3422 pobj markers,with
R1866 T3425 T3424 amod respective,markers
R1867 T3426 T3424 punct ", ",markers
R1868 T3427 T3428 compound protein,isomerase
R1869 T3428 T3424 appos isomerase,markers
R1870 T3429 T3428 compound disulfide,isomerase
R1871 T3430 T3428 punct (,isomerase
R1872 T3431 T3428 appos PDI,isomerase
R1873 T3432 T3428 punct ),isomerase
R1874 T3433 T3428 punct ", ",isomerase
R1875 T3434 T3428 conj GM130,isomerase
R1876 T3435 T3434 punct ", ",GM130
R1877 T3436 T3434 cc and,GM130
R1878 T3437 T3434 conj VVL,GM130
R1879 T3438 T3439 punct (,shown
R1880 T3439 T3393 parataxis shown,appear
R1881 T3440 T3439 nsubj data,shown
R1882 T3441 T3439 neg not,shown
R1883 T3442 T3439 punct ),shown
R1884 T3443 T3393 punct .,appear
R1885 T3445 T3446 prep By,had
R1886 T3447 T3445 pobj contrast,By
R1887 T3448 T3446 punct ", ",had
R1888 T3449 T3450 det the,markers
R1889 T3450 T3446 nsubj markers,had
R1890 T3451 T3450 amod lysosomal,markers
R1891 T3452 T3450 punct ", ",markers
R1892 T3453 T3450 appos Lysotracker,markers
R1893 T3454 T3453 cc and,Lysotracker
R1894 T3455 T3453 conj Lamp,Lysotracker
R1895 T3456 T3455 nummod 2,Lamp
R1896 T3457 T3458 advmod significantly,altered
R1897 T3458 T3459 amod altered,signal
R1898 T3459 T3446 dobj signal,had
R1899 T3460 T3446 prep in,had
R1900 T3461 T3462 amod homozygous,cells
R1901 T3462 T3460 pobj cells,in
R1902 T3463 T3462 nmod CbCln3Δex7,cells
R1903 T3464 T3463 punct /,CbCln3Δex7
R1904 T3465 T3463 nummod 8,CbCln3Δex7
R1905 T3466 T3462 punct ", ",cells
R1906 T3467 T3462 cc versus,cells
R1907 T3468 T3469 amod wild,type
R1908 T3469 T3471 compound type,cells
R1909 T3470 T3469 punct -,type
R1910 T3471 T3462 conj cells,cells
R1911 T3472 T3446 punct .,had
R1912 T3474 T3475 amod Wild,type
R1913 T3475 T3477 compound type,cells
R1914 T3476 T3475 punct -,type
R1915 T3477 T3478 nsubj cells,exhibited
R1916 T3479 T3480 advmod brightly,stained
R1917 T3480 T3481 amod stained,lysosomes
R1918 T3481 T3478 dobj lysosomes,exhibited
R1919 T3482 T3483 dep that,were
R1920 T3483 T3481 relcl were,lysosomes
R1921 T3484 T3483 acomp large,were
R1922 T3485 T3484 cc and,large
R1923 T3486 T3484 conj clustered,large
R1924 T3487 T3483 prep in,were
R1925 T3488 T3489 det the,region
R1926 T3489 T3487 pobj region,in
R1927 T3490 T3489 amod perinuclear,region
R1928 T3491 T3492 mark whereas,were
R1929 T3492 T3483 advcl were,were
R1930 T3493 T3494 amod homozygous,lysosomes
R1931 T3494 T3492 nsubj lysosomes,were
R1932 T3495 T3494 nmod CbCln3Δex7,lysosomes
R1933 T3496 T3495 punct /,CbCln3Δex7
R1934 T3497 T3495 nummod 8,CbCln3Δex7
R1935 T3498 T3499 advmod lightly,stained
R1936 T3499 T3500 amod stained,vesicles
R1937 T3500 T3492 attr vesicles,were
R1938 T3501 T3500 punct ", ",vesicles
R1939 T3502 T3500 amod smaller,vesicles
R1940 T3503 T3504 dep that,scattered
R1941 T3504 T3500 relcl scattered,vesicles
R1942 T3505 T3504 auxpass were,scattered
R1943 T3506 T3507 advmod more,diffusely
R1944 T3507 T3504 advmod diffusely,scattered
R1945 T3508 T3504 prep in,scattered
R1946 T3509 T3510 det the,cytoplasm
R1947 T3510 T3508 pobj cytoplasm,in
R1948 T3511 T3510 prep of,cytoplasm
R1949 T3512 T3513 det the,cell
R1950 T3513 T3511 pobj cell,of
R1951 T3514 T3515 punct (,Fig.
R1952 T3515 T3478 parataxis Fig.,exhibited
R1953 T3516 T3515 nummod 6,Fig.
R1954 T3517 T3515 punct ),Fig.
R1955 T3518 T3478 punct .,exhibited
R1956 T3520 T3521 nmod Lamp,distribution
R1957 T3521 T3523 nsubjpass distribution,altered
R1958 T3522 T3520 nummod 1,Lamp
R1959 T3524 T3523 auxpass was,altered
R1960 T3525 T3523 advmod also,altered
R1961 T3526 T3523 punct ", ",altered
R1962 T3527 T3528 mark as,noted
R1963 T3528 T3523 advcl noted,altered
R1964 T3529 T3528 advmod previously,noted
R1965 T3530 T3531 punct (,Fig.
R1966 T3531 T3523 parataxis Fig.,altered
R1967 T3532 T3531 nummod 3,Fig.
R1968 T3533 T3531 punct ),Fig.
R1969 T3534 T3523 punct .,altered
R1970 T3536 T3537 advmod However,were
R1971 T3538 T3537 punct ", ",were
R1972 T3539 T3540 nmod Lamp,levels
R1973 T3540 T3537 nsubj levels,were
R1974 T3541 T3539 nummod 1,Lamp
R1975 T3542 T3539 cc and,Lamp
R1976 T3543 T3539 conj Lamp,Lamp
R1977 T3544 T3543 nummod 2,Lamp
R1978 T3545 T3546 amod total,protein
R1979 T3546 T3540 compound protein,levels
R1980 T3547 T3537 acomp similar,were
R1981 T3548 T3537 prep in,were
R1982 T3549 T3550 amod wild,type
R1983 T3550 T3552 nmod type,cells
R1984 T3551 T3550 punct -,type
R1985 T3552 T3548 pobj cells,in
R1986 T3553 T3550 cc and,type
R1987 T3554 T3550 conj homozygous,type
R1988 T3555 T3552 nmod CbCln3Δex7,cells
R1989 T3556 T3555 punct /,CbCln3Δex7
R1990 T3557 T3555 nummod 8,CbCln3Δex7
R1991 T3558 T3537 prep by,were
R1992 T3559 T3560 compound immunoblot,analysis
R1993 T3560 T3558 pobj analysis,by
R1994 T3561 T3537 punct ", ",were
R1995 T3562 T3537 advcl indicating,were
R1996 T3563 T3564 det the,signal
R1997 T3564 T3566 nsubj signal,reflects
R1998 T3565 T3564 amod altered,signal
R1999 T3566 T3562 advcl reflects,indicating
R2000 T3567 T3566 advmod likely,reflects
R2001 T3568 T3569 amod dispersed,lysosomes
R2002 T3569 T3566 dobj lysosomes,reflects
R2003 T3570 T3569 cc or,lysosomes
R2004 T3571 T3572 amod altered,localization
R2005 T3572 T3569 conj localization,lysosomes
R2006 T3573 T3572 cc and,localization
R2007 T3574 T3572 punct /,localization
R2008 T3575 T3572 cc or,localization
R2009 T3576 T3577 compound epitope,availability
R2010 T3577 T3572 conj availability,localization
R2011 T3578 T3579 punct (,shown
R2012 T3579 T3562 parataxis shown,indicating
R2013 T3580 T3579 nsubj data,shown
R2014 T3581 T3579 neg not,shown
R2015 T3582 T3579 punct ),shown
R2016 T3583 T3537 punct .,were
R2017 T3585 T3586 nsubj It,is
R2018 T3587 T3586 acomp noteworthy,is
R2019 T3588 T3589 mark that,exhibited
R2020 T3589 T3586 ccomp exhibited,is
R2021 T3590 T3591 compound Lysotracker,dye
R2022 T3591 T3589 nsubj dye,exhibited
R2023 T3592 T3591 punct ", ",dye
R2024 T3593 T3594 dep which,accumulates
R2025 T3594 T3591 relcl accumulates,dye
R2026 T3595 T3594 advmod selectively,accumulates
R2027 T3596 T3594 prep in,accumulates
R2028 T3597 T3598 amod acidic,compartments
R2029 T3598 T3596 pobj compartments,in
R2030 T3599 T3589 punct ", ",exhibited
R2031 T3600 T3601 det the,reduction
R2032 T3601 T3589 dobj reduction,exhibited
R2033 T3602 T3603 advmod most,marked
R2034 T3603 T3601 amod marked,reduction
R2035 T3604 T3601 prep in,reduction
R2036 T3605 T3606 amod lysosomal,labeling
R2037 T3606 T3604 pobj labeling,in
R2038 T3607 T3586 punct .,is
R2039 T3609 T3610 det This,observation
R2040 T3610 T3611 nsubj observation,reflect
R2041 T3612 T3611 aux may,reflect
R2042 T3613 T3614 amod altered,pH
R2043 T3614 T3611 dobj pH,reflect
R2044 T3615 T3614 amod lysosomal,pH
R2045 T3616 T3614 punct ", ",pH
R2046 T3617 T3618 det an,finding
R2047 T3618 T3614 appos finding,pH
R2048 T3619 T3618 amod established,finding
R2049 T3620 T3618 prep in,finding
R2050 T3621 T3620 pobj JNCL,in
R2051 T3622 T3623 punct [,15
R2052 T3623 T3611 parataxis 15,reflect
R2053 T3624 T3623 nummod 10,15
R2054 T3625 T3623 punct ",",15
R2055 T3626 T3623 punct ],15
R2056 T3627 T3611 punct .,reflect
R2057 T3632 T3631 nmod C,o
R2058 T3633 T3630 nsubjpass n,w
R2059 T3635 T3633 prep i,n
R2060 T3636 T3637 nmod ste,t
R2061 T3637 T3635 pobj t,i
R2062 T3638 T3636 cc n,ste
R2063 T3640 T3641 amod i,t
R2064 T3645 T3646 amod h,e
R2065 T3663 T3661 amod o,me
R2066 T3669 T3668 amod r,signal
R2067 T3670 T3668 nmod ker,signal
R2068 T3671 T3670 punct (,ker
R2069 T3672 T3670 appos EEA1,ker
R2070 T3673 T3668 punct ),signal
R2071 T3674 T3668 acl observed,signal
R2072 T3675 T3674 prep by,observed
R2073 T3676 T3675 pobj immunostaining,by
R2074 T3677 T3678 punct (,Fig.
R2075 T3679 T3678 nummod 3,Fig.
R2076 T3680 T3678 punct ),Fig.
R2077 T3681 T3667 punct ", ",altered
R2078 T3682 T3683 compound fluid,phase
R2079 T3683 T3685 compound phase,endocytosis
R2080 T3684 T3683 punct -,phase
R2081 T3685 T3667 nsubjpass endocytosis,altered
R2082 T3686 T3667 auxpass was,altered
R2083 T3687 T3667 advmod also,altered
R2084 T3688 T3667 prep in,altered
R2085 T3689 T3690 amod homozygous,cells
R2086 T3690 T3688 pobj cells,in
R2087 T3691 T3690 nmod CbCln3Δex7,cells
R2088 T3692 T3691 punct /,CbCln3Δex7
R2089 T3693 T3691 nummod 8,CbCln3Δex7
R2090 T3694 T3667 punct ", ",altered
R2091 T3695 T3696 mark as,measured
R2092 T3696 T3667 advcl measured,altered
R2093 T3697 T3696 agent by,measured
R2094 T3698 T3699 compound dextran,FITC
R2095 T3699 T3701 compound FITC,uptake
R2096 T3700 T3699 punct -,FITC
R2097 T3701 T3697 pobj uptake,by
R2098 T3702 T3703 punct (,Fig.
R2099 T3703 T3667 parataxis Fig.,altered
R2100 T3704 T3703 nummod 7,Fig.
R2101 T3705 T3703 punct ),Fig.
R2102 T3706 T3667 punct .,altered
R2103 T3708 T3709 prep Following,displayed
R2104 T3710 T3711 det a,incubation
R2105 T3711 T3708 pobj incubation,Following
R2106 T3712 T3713 nummod 15,minute
R2107 T3713 T3711 compound minute,incubation
R2108 T3714 T3713 punct -,minute
R2109 T3715 T3711 prep in,incubation
R2110 T3716 T3715 pobj media,in
R2111 T3717 T3716 acl containing,media
R2112 T3718 T3719 compound dextran,FITC
R2113 T3719 T3717 dobj FITC,containing
R2114 T3720 T3719 punct -,FITC
R2115 T3721 T3709 punct ", ",displayed
R2116 T3722 T3723 amod wild,type
R2117 T3723 T3725 nmod type,cells
R2118 T3724 T3723 punct -,type
R2119 T3725 T3709 nsubj cells,displayed
R2120 T3726 T3723 cc and,type
R2121 T3727 T3723 conj heterozygote,type
R2122 T3728 T3729 advmod brightly,stained
R2123 T3729 T3730 amod stained,vesicles
R2124 T3730 T3709 dobj vesicles,displayed
R2125 T3731 T3730 punct ", ",vesicles
R2126 T3732 T3730 amod large,vesicles
R2127 T3733 T3730 amod endocytic,vesicles
R2128 T3734 T3735 dep that,clustered
R2129 T3735 T3730 relcl clustered,vesicles
R2130 T3736 T3735 auxpass were,clustered
R2131 T3737 T3735 prep in,clustered
R2132 T3738 T3739 det the,region
R2133 T3739 T3737 pobj region,in
R2134 T3740 T3739 amod perinuclear,region
R2135 T3741 T3709 punct .,displayed
R2136 T3743 T3744 advmod However,stained
R2137 T3745 T3744 punct ", ",stained
R2138 T3746 T3747 amod homozygous,cells
R2139 T3747 T3744 nsubjpass cells,stained
R2140 T3748 T3747 nmod CbCln3Δex7,cells
R2141 T3749 T3748 punct /,CbCln3Δex7
R2142 T3750 T3748 nummod 8,CbCln3Δex7
R2143 T3751 T3744 auxpass were,stained
R2144 T3752 T3753 advmod less,brightly
R2145 T3753 T3744 advmod brightly,stained
R2146 T3754 T3744 prep with,stained
R2147 T3755 T3756 amod most,signal
R2148 T3756 T3760 nsubj signal,localizing
R2149 T3757 T3758 compound dextran,FITC
R2150 T3758 T3756 compound FITC,signal
R2151 T3759 T3758 punct -,FITC
R2152 T3760 T3754 pcomp localizing,with
R2153 T3761 T3760 prep to,localizing
R2154 T3762 T3763 amod smaller,vesicles
R2155 T3763 T3761 pobj vesicles,to
R2156 T3764 T3763 acl scattered,vesicles
R2157 T3765 T3764 prep throughout,scattered
R2158 T3766 T3767 det the,cytoplasm
R2159 T3767 T3765 pobj cytoplasm,throughout
R2160 T3768 T3767 prep of,cytoplasm
R2161 T3769 T3770 det the,cell
R2162 T3770 T3768 pobj cell,of
R2163 T3771 T3744 punct .,stained
R2164 T3778 T3776 nmod l,l
R2165 T3780 T3779 punct ", ",y
R2166 T3792 T3793 amod u,n
R2167 T3793 T3794 nmod n,c
R2168 T3808 T3807 amod hondrial,protein
R2169 T3810 T3811 poss its,turnover
R2170 T3811 T3812 nsubj turnover,proceeds
R2171 T3813 T3812 prep through,proceeds
R2172 T3814 T3815 amod autophagic,engulfment
R2173 T3815 T3813 pobj engulfment,through
R2174 T3816 T3815 prep of,engulfment
R2175 T3817 T3816 pobj mitochondria,of
R2176 T3818 T3819 punct [,13
R2177 T3819 T3812 parataxis 13,proceeds
R2178 T3820 T3819 punct ],13
R2179 T3821 T3806 punct ", ",analyzed
R2180 T3822 T3806 nsubj we,analyzed
R2181 T3823 T3824 amod homozygous,CbCln3Δex7
R2182 T3824 T3806 dobj CbCln3Δex7,analyzed
R2183 T3825 T3824 punct /,CbCln3Δex7
R2184 T3826 T3824 nummod 8,CbCln3Δex7
R2185 T3827 T3828 npadvmod cell,mitochondrial
R2186 T3828 T3824 amod mitochondrial,CbCln3Δex7
R2187 T3829 T3824 appos morphology,CbCln3Δex7
R2188 T3830 T3829 cc and,morphology
R2189 T3831 T3829 conj function,morphology
R2190 T3832 T3806 punct .,analyzed
R2191 T3834 T3835 amod Mitochondrial,distribution
R2192 T3835 T3836 nsubj distribution,was
R2193 T3836 T3843 ccomp was,appeared
R2194 T3837 T3835 prep in,distribution
R2195 T3838 T3839 amod homozygous,cells
R2196 T3839 T3837 pobj cells,in
R2197 T3840 T3839 nmod CbCln3Δex7,cells
R2198 T3841 T3840 punct /,CbCln3Δex7
R2199 T3842 T3840 nummod 8,CbCln3Δex7
R2200 T3844 T3836 acomp indistinguishable,was
R2201 T3845 T3844 prep from,indistinguishable
R2202 T3846 T3847 amod wild,type
R2203 T3847 T3849 nmod type,cells
R2204 T3848 T3847 punct -,type
R2205 T3849 T3845 pobj cells,from
R2206 T3850 T3847 cc and,type
R2207 T3851 T3847 conj heterozygous,type
R2208 T3852 T3843 punct ;,appeared
R2209 T3853 T3843 advmod however,appeared
R2210 T3854 T3843 punct ", ",appeared
R2211 T3855 T3856 amod homozygous,mitochondria
R2212 T3856 T3843 nsubj mitochondria,appeared
R2213 T3857 T3856 nmod CbCln3Δex7,mitochondria
R2214 T3858 T3857 punct /,CbCln3Δex7
R2215 T3859 T3857 nummod 8,CbCln3Δex7
R2216 T3860 T3861 advmod more,elongated
R2217 T3861 T3843 oprd elongated,appeared
R2218 T3862 T3861 prep by,elongated
R2219 T3863 T3864 compound grp75,immunostaining
R2220 T3864 T3862 pobj immunostaining,by
R2221 T3865 T3864 compound marker,immunostaining
R2222 T3866 T3864 cc and,immunostaining
R2223 T3867 T3868 compound TEM,analysis
R2224 T3868 T3864 conj analysis,immunostaining
R2225 T3869 T3870 punct (,8a
R2226 T3870 T3843 parataxis 8a,appeared
R2227 T3871 T3870 compound Fig.,8a
R2228 T3872 T3870 punct ),8a
R2229 T3873 T3843 punct .,appeared
R2230 T3875 T3876 nummod 72,%
R2231 T3876 T3877 nsubj %,were
R2232 T3878 T3876 prep of,%
R2233 T3879 T3880 amod homozygous,mitochondria
R2234 T3880 T3878 pobj mitochondria,of
R2235 T3881 T3880 compound mutant,mitochondria
R2236 T3882 T3883 amod greater,0.6
R2237 T3883 T3885 nummod 0.6,μm
R2238 T3884 T3883 quantmod than,0.6
R2239 T3885 T3877 attr μm,were
R2240 T3886 T3885 prep in,μm
R2241 T3887 T3886 pobj length,in
R2242 T3888 T3889 punct (,μm
R2243 T3889 T3877 parataxis μm,were
R2244 T3890 T3889 nsubj range,μm
R2245 T3891 T3889 punct =,μm
R2246 T3892 T3889 nummod 0.26,μm
R2247 T3893 T3889 prep to,μm
R2248 T3894 T3895 nummod 2.75,μm
R2249 T3895 T3893 pobj μm,to
R2250 T3896 T3889 punct ),μm
R2251 T3897 T3877 punct ", ",were
R2252 T3898 T3899 mark while,reached
R2253 T3899 T3877 advcl reached,were
R2254 T3900 T3901 amod fewer,mitochondria
R2255 T3901 T3899 nsubj mitochondria,reached
R2256 T3902 T3903 amod wild,type
R2257 T3903 T3901 compound type,mitochondria
R2258 T3904 T3903 punct -,type
R2259 T3905 T3906 punct (,%
R2260 T3906 T3901 parataxis %,mitochondria
R2261 T3907 T3906 nummod 51,%
R2262 T3908 T3906 punct ),%
R2263 T3909 T3910 det this,length
R2264 T3910 T3899 dobj length,reached
R2265 T3911 T3912 punct (,μm
R2266 T3912 T3899 parataxis μm,reached
R2267 T3913 T3912 nsubj range,μm
R2268 T3914 T3912 punct =,μm
R2269 T3915 T3912 nummod 0.15,μm
R2270 T3916 T3912 prep to,μm
R2271 T3917 T3918 nummod 2.29,μm
R2272 T3918 T3916 pobj μm,to
R2273 T3919 T3912 punct ),μm
R2274 T3920 T3877 punct .,were
R2275 T3922 T3923 amod Mitochondrial,width
R2276 T3923 T3924 nsubjpass width,altered
R2277 T3925 T3924 auxpass was,altered
R2278 T3926 T3924 neg not,altered
R2279 T3927 T3924 prep in,altered
R2280 T3928 T3929 amod homozygous,cells
R2281 T3929 T3927 pobj cells,in
R2282 T3930 T3929 nmod CbCln3Δex7,cells
R2283 T3931 T3930 punct /,CbCln3Δex7
R2284 T3932 T3930 nummod 8,CbCln3Δex7
R2285 T3933 T3934 punct (,shown
R2286 T3934 T3924 parataxis shown,altered
R2287 T3935 T3934 nsubj data,shown
R2288 T3936 T3934 neg not,shown
R2289 T3937 T3934 punct ),shown
R2290 T3938 T3924 punct .,altered
R2291 T3940 T3941 advmod Moreover,had
R2292 T3942 T3941 punct ", ",had
R2293 T3943 T3941 prep compared,had
R2294 T3944 T3943 prep to,compared
R2295 T3945 T3946 amod wild,type
R2296 T3946 T3948 nmod type,cells
R2297 T3947 T3946 punct -,type
R2298 T3948 T3944 pobj cells,to
R2299 T3949 T3946 cc or,type
R2300 T3950 T3946 conj heterozygous,type
R2301 T3951 T3941 punct ", ",had
R2302 T3952 T3953 amod homozygous,cells
R2303 T3953 T3941 nsubj cells,had
R2304 T3954 T3953 nmod CbCln3Δex7,cells
R2305 T3955 T3954 punct /,CbCln3Δex7
R2306 T3956 T3954 nummod 8,CbCln3Δex7
R2307 T3957 T3958 advmod significantly,reduced
R2308 T3958 T3959 amod reduced,levels
R2309 T3959 T3941 dobj levels,had
R2310 T3960 T3961 amod cellular,ATP
R2311 T3961 T3959 compound ATP,levels
R2312 T3962 T3963 punct (,Fig.
R2313 T3963 T3941 parataxis Fig.,had
R2314 T3964 T3965 npadvmod 1.3,less
R2315 T3965 T3963 amod less,Fig.
R2316 T3966 T3964 advmod fold,1.3
R2317 T3967 T3963 punct ", ",Fig.
R2318 T3968 T3963 nummod 8b,Fig.
R2319 T3969 T3963 punct ),Fig.
R2320 T3970 T3941 cc and,had
R2321 T3971 T3941 conj exhibited,had
R2322 T3972 T3973 amod reduced,survival
R2323 T3973 T3971 dobj survival,exhibited
R2324 T3974 T3971 prep following,exhibited
R2325 T3975 T3976 compound hydrogen,peroxide
R2326 T3976 T3977 compound peroxide,treatment
R2327 T3977 T3974 pobj treatment,following
R2328 T3978 T3979 punct (,8c
R2329 T3979 T3971 parataxis 8c,exhibited
R2330 T3980 T3981 punct ~,50
R2331 T3981 T3982 nummod 50,%
R2332 T3982 T3979 dep %,8c
R2333 T3983 T3982 prep of,%
R2334 T3984 T3985 amod wild,type
R2335 T3985 T3987 compound type,survival
R2336 T3986 T3985 punct -,type
R2337 T3987 T3983 pobj survival,of
R2338 T3988 T3979 punct ", ",8c
R2339 T3989 T3979 compound Fig.,8c
R2340 T3990 T3979 punct ),8c
R2341 T3991 T3941 punct ", ",had
R2342 T3992 T3941 advcl suggesting,had
R2343 T3993 T3994 amod impaired,metabolism
R2344 T3994 T3992 dobj metabolism,suggesting
R2345 T3995 T3994 compound energy,metabolism
R2346 T3996 T3994 cc and,metabolism
R2347 T3997 T3998 amod oxidative,response
R2348 T3998 T3994 conj response,metabolism
R2349 T3999 T3998 compound stress,response
R2350 T4000 T3941 punct .,had
R2351 T4002 T4003 advcl Taken,support
R2352 T4004 T4002 advmod together,Taken
R2353 T4005 T4003 punct ", ",support
R2354 T4006 T4007 det these,data
R2355 T4007 T4003 nsubj data,support
R2356 T4008 T4009 amod impaired,function
R2357 T4009 T4003 dobj function,support
R2358 T4010 T4009 amod mitochondrial,function
R2359 T4011 T4009 prep in,function
R2360 T4012 T4013 amod homozygous,cells
R2361 T4013 T4011 pobj cells,in
R2362 T4014 T4013 nmod CbCln3Δex7,cells
R2363 T4015 T4014 punct /,CbCln3Δex7
R2364 T4016 T4014 nummod 8,CbCln3Δex7
R2365 T4017 T4003 punct .,support
R5056 T7982 T7983 amod Neuronal,marker
R5057 T7983 T7984 compound marker,expression
R5058 T7984 T7985 dep expression,shown
R5059 T7986 T7984 prep in,expression
R5060 T7987 T7988 nmod CbCln3,cells
R5061 T7988 T7986 pobj cells,in
R5062 T7989 T7987 punct +,CbCln3
R5063 T7990 T7987 punct /,CbCln3
R5064 T7991 T7987 punct +,CbCln3
R5065 T7992 T7985 nsubjpass Characterization,shown
R5066 T7993 T7992 prep of,Characterization
R5067 T7994 T7995 nmod CbCln3,cells
R5068 T7995 T7993 pobj cells,of
R5069 T7996 T7994 punct +,CbCln3
R5070 T7997 T7994 punct /,CbCln3
R5071 T7998 T7994 punct +,CbCln3
R5072 T7999 T7992 prep by,Characterization
R5073 T8000 T7999 pobj immunofluorescence,by
R5074 T8001 T8000 prep with,immunofluorescence
R5075 T8002 T8003 compound marker,antibodies
R5076 T8003 T8001 pobj antibodies,with
R5077 T8004 T7985 auxpass is,shown
R5078 T8005 T7985 punct .,shown
R5079 T8007 T8008 nmod CbCln3,precursors
R5080 T8008 T8012 nsubj precursors,exhibit
R5081 T8009 T8007 punct +,CbCln3
R5082 T8010 T8007 punct /,CbCln3
R5083 T8011 T8007 punct +,CbCln3
R5084 T8013 T8014 compound nestin,expression
R5085 T8014 T8012 dobj expression,exhibit
R5086 T8015 T8016 punct (,a
R5087 T8016 T8014 parataxis a,expression
R5088 T8017 T8016 punct ),a
R5089 T8018 T8014 cc but,expression
R5090 T8019 T8018 neg not,but
R5091 T8020 T8021 compound GFAP,expression
R5092 T8021 T8014 conj expression,expression
R5093 T8022 T8023 punct (,b
R5094 T8023 T8021 parataxis b,expression
R5095 T8024 T8023 punct ),b
R5096 T8025 T8012 punct ", ",exhibit
R5097 T8026 T8012 advcl consistent,exhibit
R5098 T8027 T8026 prep with,consistent
R5099 T8028 T8029 det a,identity
R5100 T8029 T8027 pobj identity,with
R5101 T8030 T8029 amod neuronal,identity
R5102 T8031 T8029 compound precursor,identity
R5103 T8032 T8012 punct .,exhibit
R5104 T8034 T8035 prep Upon,achieved
R5105 T8036 T8034 pobj stimulation,Upon
R5106 T8037 T8036 prep with,stimulation
R5107 T8038 T8039 det a,cocktail
R5108 T8039 T8037 pobj cocktail,with
R5109 T8040 T8039 compound differentiation,cocktail
R5110 T8041 T8042 punct (,see
R5111 T8042 T8036 parataxis see,stimulation
R5112 T8043 T8042 dobj Methods,see
R5113 T8044 T8042 punct ),see
R5114 T8045 T8035 punct ", ",achieved
R5115 T8046 T8047 nmod CbCln3,cells
R5116 T8047 T8035 nsubj cells,achieved
R5117 T8048 T8046 punct +,CbCln3
R5118 T8049 T8046 punct /,CbCln3
R5119 T8050 T8046 punct +,CbCln3
R5120 T8051 T8052 npadvmod neuron,like
R5121 T8052 T8054 amod like,morphology
R5122 T8053 T8052 punct -,like
R5123 T8054 T8035 dobj morphology,achieved
R5124 T8055 T8035 punct ", ",achieved
R5125 T8056 T8035 prep with,achieved
R5126 T8057 T8058 amod rounded,bodies
R5127 T8058 T8056 pobj bodies,with
R5128 T8059 T8058 compound cell,bodies
R5129 T8060 T8058 cc and,bodies
R5130 T8061 T8058 conj extension,bodies
R5131 T8062 T8061 prep of,extension
R5132 T8063 T8062 pobj processes,of
R5133 T8064 T8035 punct ", ",achieved
R5134 T8065 T8035 cc and,achieved
R5135 T8066 T8067 nmod MAP2,expression
R5136 T8067 T8076 nsubjpass expression,increased
R5137 T8068 T8069 punct (,c
R5138 T8069 T8066 parataxis c,MAP2
R5139 T8070 T8069 punct ),c
R5140 T8071 T8066 cc and,MAP2
R5141 T8072 T8066 conj NeuN,MAP2
R5142 T8073 T8074 punct (,d
R5143 T8074 T8072 parataxis d,NeuN
R5144 T8075 T8074 punct ),d
R5145 T8076 T8035 conj increased,achieved
R5146 T8077 T8076 auxpass was,increased
R5147 T8078 T8076 punct .,increased
R5148 T8080 T8081 nmod CbCln3,cells
R5149 T8081 T8085 nsubj cells,are
R5150 T8082 T8080 punct +,CbCln3
R5151 T8083 T8080 punct /,CbCln3
R5152 T8084 T8080 punct +,CbCln3
R5153 T8086 T8085 acomp negative,are
R5154 T8087 T8086 prep for,negative
R5155 T8088 T8089 det the,marker
R5156 T8089 T8087 pobj marker,for
R5157 T8090 T8089 compound Purkinje,marker
R5158 T8091 T8089 compound neuron,marker
R5159 T8092 T8089 appos calbindin,marker
R5160 T8093 T8094 punct (,e
R5161 T8094 T8089 parataxis e,marker
R5162 T8095 T8094 punct ),e
R5163 T8096 T8085 punct .,are
R5164 T8098 T8099 nmod CbCln3,Δex7
R5165 T8099 T8102 nmod Δex7,lines
R5166 T8100 T8099 punct +,Δex7
R5167 T8101 T8099 punct /,Δex7
R5168 T8102 T8114 nsubj lines,exhibited
R5169 T8103 T8099 punct /,Δex7
R5170 T8104 T8099 nummod 8,Δex7
R5171 T8105 T8099 cc and,Δex7
R5172 T8106 T8107 nmod CbCln3Δex7,Δex7
R5173 T8107 T8099 conj Δex7,Δex7
R5174 T8108 T8107 punct /,Δex7
R5175 T8109 T8107 nummod 8,Δex7
R5176 T8110 T8107 punct /,Δex7
R5177 T8111 T8107 punct /,Δex7
R5178 T8112 T8107 nummod 8,Δex7
R5179 T8113 T8102 compound cell,lines
R5180 T8115 T8116 amod identical,results
R5181 T8116 T8114 dobj results,exhibited
R5182 T8117 T8118 compound marker,immunofluorescence
R5183 T8118 T8116 compound immunofluorescence,results
R5184 T8119 T8114 punct .,exhibited
R5185 T8121 T8122 meta a,magnification
R5186 T8123 T8121 punct ", ",a
R5187 T8124 T8121 dep b,a
R5188 T8125 T8121 punct ),a
R5189 T8126 T8122 nummod 20,magnification
R5190 T8127 T8126 punct ×,20
R5191 T8128 T8122 punct ;,magnification
R5192 T8129 T8130 meta c,magnification
R5193 T8130 T8122 appos magnification,magnification
R5194 T8131 T8129 punct ", ",c
R5195 T8132 T8129 dep d,c
R5196 T8133 T8129 punct ", ",c
R5197 T8134 T8129 dep e,c
R5198 T8135 T8129 punct ),c
R5199 T8136 T8130 nummod 40,magnification
R5200 T8137 T8136 punct ×,40
R5201 T8138 T8122 punct .,magnification
R5202 T8215 T8216 compound RT,PCR
R5203 T8216 T8218 dep PCR,shown
R5204 T8217 T8216 punct -,PCR
R5205 T8219 T8216 prep of,PCR
R5206 T8220 T8221 compound Cln3,mRNA
R5207 T8221 T8219 pobj mRNA,of
R5208 T8222 T8216 prep in,PCR
R5209 T8223 T8224 amod wild,type
R5210 T8224 T8226 nmod type,cells
R5211 T8225 T8224 punct -,type
R5212 T8226 T8222 pobj cells,in
R5213 T8227 T8224 cc and,type
R5214 T8228 T8224 conj homozygous,type
R5215 T8229 T8226 nmod CbCln3Δex7,cells
R5216 T8230 T8229 punct /,CbCln3Δex7
R5217 T8231 T8229 nummod 8,CbCln3Δex7
R5218 T8232 T8233 nmod Cln3,products
R5219 T8233 T8218 nsubjpass products,shown
R5220 T8234 T8235 npadvmod Exon1,forward
R5221 T8235 T8237 amod forward,reverse
R5222 T8236 T8235 punct -,forward
R5223 T8237 T8233 nmod reverse,products
R5224 T8238 T8237 punct ", ",reverse
R5225 T8239 T8237 npadvmod Exon,reverse
R5226 T8240 T8239 nummod 15,Exon
R5227 T8241 T8237 punct -,reverse
R5228 T8242 T8243 compound RT,PCR
R5229 T8243 T8233 compound PCR,products
R5230 T8244 T8243 punct -,PCR
R5231 T8245 T8218 auxpass are,shown
R5232 T8246 T8218 punct ", ",shown
R5233 T8247 T8218 prep from,shown
R5234 T8248 T8249 amod total,RNA
R5235 T8249 T8247 pobj RNA,from
R5236 T8250 T8251 amod wild,type
R5237 T8251 T8249 nmod type,RNA
R5238 T8252 T8251 punct -,type
R5239 T8253 T8251 punct (,type
R5240 T8254 T8255 punct +,+
R5241 T8255 T8251 appos +,type
R5242 T8256 T8255 punct /,+
R5243 T8257 T8251 punct ),type
R5244 T8258 T8251 cc or,type
R5245 T8259 T8260 amod homozygous,mutant
R5246 T8260 T8251 conj mutant,type
R5247 T8261 T8260 punct (,mutant
R5248 T8262 T8260 appos Δex7,mutant
R5249 T8263 T8262 punct /,Δex7
R5250 T8264 T8262 nummod 8,Δex7
R5251 T8265 T8262 punct /,Δex7
R5252 T8266 T8262 appos Δex7,Δex7
R5253 T8267 T8266 punct /,Δex7
R5254 T8268 T8266 nummod 8,Δex7
R5255 T8269 T8249 punct ),RNA
R5256 T8270 T8249 nmod brain,RNA
R5257 T8271 T8270 cc and,brain
R5258 T8272 T8273 compound cell,line
R5259 T8273 T8270 conj line,brain
R5260 T8274 T8218 punct .,shown
R5261 T8276 T8277 nmod Brain,products
R5262 T8277 T8285 nsubj products,had
R5263 T8278 T8276 cc and,Brain
R5264 T8279 T8280 compound cell,line
R5265 T8280 T8276 conj line,Brain
R5266 T8281 T8282 compound RT,PCR
R5267 T8282 T8277 compound PCR,products
R5268 T8283 T8282 punct -,PCR
R5269 T8284 T8277 compound reaction,products
R5270 T8286 T8287 amod identical,patterns
R5271 T8287 T8285 dobj patterns,had
R5272 T8288 T8287 compound band,patterns
R5273 T8289 T8285 prep on,had
R5274 T8290 T8291 compound ethidium,bromide
R5275 T8291 T8293 npadvmod bromide,stained
R5276 T8292 T8291 punct -,bromide
R5277 T8293 T8294 amod stained,gels
R5278 T8294 T8289 pobj gels,on
R5279 T8295 T8294 compound agarose,gels
R5280 T8296 T8285 punct .,had
R5281 T8298 T8299 amod Wild,type
R5282 T8299 T8301 compound type,product
R5283 T8300 T8299 punct -,type
R5284 T8301 T8305 nsubj product,was
R5285 T8302 T8303 compound RT,PCR
R5286 T8303 T8301 compound PCR,product
R5287 T8304 T8303 punct -,PCR
R5288 T8306 T8307 det a,band
R5289 T8307 T8305 attr band,was
R5290 T8308 T8307 amod single,band
R5291 T8309 T8310 punct ~,1.6
R5292 T8310 T8311 nummod 1.6,kb
R5293 T8311 T8307 compound kb,band
R5294 T8312 T8305 cc and,was
R5295 T8313 T8314 compound mutant,products
R5296 T8314 T8315 nsubj products,were
R5297 T8315 T8305 conj were,was
R5298 T8316 T8317 punct ~,1.6
R5299 T8317 T8318 nummod 1.6,kb
R5300 T8318 T8315 attr kb,were
R5301 T8319 T8317 punct ", ",1.6
R5302 T8320 T8321 punct ~,1.5
R5303 T8321 T8317 conj 1.5,1.6
R5304 T8322 T8321 punct ", ",1.5
R5305 T8323 T8324 punct ~,1.4
R5306 T8324 T8321 conj 1.4,1.5
R5307 T8325 T8324 punct ", ",1.4
R5308 T8326 T8327 punct ~,1.35
R5309 T8327 T8324 conj 1.35,1.4
R5310 T8328 T8327 punct ", ",1.35
R5311 T8329 T8327 cc and,1.35
R5312 T8330 T8331 punct ~,1.3
R5313 T8331 T8327 conj 1.3,1.35
R5314 T8332 T8315 punct ", ",were
R5315 T8333 T8315 advcl representing,were
R5316 T8334 T8335 amod multiple,variants
R5317 T8335 T8333 dobj variants,representing
R5318 T8336 T8337 compound mutant,splice
R5319 T8337 T8335 compound splice,variants
R5320 T8338 T8315 punct .,were
R5321 T8445 T8446 nmod Battenin,marker
R5322 T8446 T8451 compound marker,co-staining
R5323 T8447 T8445 cc and,Battenin
R5324 T8448 T8445 conj lysosomal,Battenin
R5325 T8449 T8448 cc and,lysosomal
R5326 T8450 T8448 conj endosomal,lysosomal
R5327 T8451 T8452 dep co-staining,shown
R5328 T8453 T8451 prep in,co-staining
R5329 T8454 T8455 amod wild,type
R5330 T8455 T8457 nmod type,cells
R5331 T8456 T8455 punct -,type
R5332 T8457 T8453 pobj cells,in
R5333 T8458 T8455 cc and,type
R5334 T8459 T8455 conj homozygous,type
R5335 T8460 T8461 nmod CbCln3Δex7,precursor
R5336 T8461 T8457 compound precursor,cells
R5337 T8462 T8460 punct /,CbCln3Δex7
R5338 T8463 T8460 nummod 8,CbCln3Δex7
R5339 T8464 T8461 amod cerebellar,precursor
R5340 T8465 T8466 compound Batp1,immunostaining
R5341 T8466 T8452 nsubjpass immunostaining,shown
R5342 T8467 T8466 prep of,immunostaining
R5343 T8468 T8469 amod wild,type
R5344 T8469 T8467 pobj type,of
R5345 T8470 T8469 punct -,type
R5346 T8471 T8469 punct (,type
R5347 T8472 T8469 appos CbCln3,type
R5348 T8473 T8472 punct +,CbCln3
R5349 T8474 T8472 punct /,CbCln3
R5350 T8475 T8472 punct +,CbCln3
R5351 T8476 T8469 punct ),type
R5352 T8477 T8469 cc and,type
R5353 T8478 T8479 amod homozygous,mutant
R5354 T8479 T8480 nmod mutant,cells
R5355 T8480 T8469 conj cells,type
R5356 T8481 T8479 punct (,mutant
R5357 T8482 T8483 nmod CbCln3Δex7,Δex7
R5358 T8483 T8479 appos Δex7,mutant
R5359 T8484 T8483 punct /,Δex7
R5360 T8485 T8483 nummod 8,Δex7
R5361 T8486 T8483 punct /,Δex7
R5362 T8487 T8483 punct /,Δex7
R5363 T8488 T8483 nummod 8,Δex7
R5364 T8489 T8480 punct ),cells
R5365 T8490 T8480 amod cerebellar,cells
R5366 T8491 T8480 compound precursor,cells
R5367 T8492 T8452 auxpass is,shown
R5368 T8493 T8452 punct ", ",shown
R5369 T8494 T8452 prep with,shown
R5370 T8495 T8494 pobj co-staining,with
R5371 T8496 T8495 prep for,co-staining
R5372 T8497 T8496 pobj lysosomes,for
R5373 T8498 T8497 punct (,lysosomes
R5374 T8499 T8497 appos Lamp,lysosomes
R5375 T8500 T8499 nummod 1,Lamp
R5376 T8501 T8497 punct ),lysosomes
R5377 T8502 T8497 punct ", ",lysosomes
R5378 T8503 T8504 amod early,endosomes
R5379 T8504 T8497 conj endosomes,lysosomes
R5380 T8505 T8504 punct (,endosomes
R5381 T8506 T8504 appos EEA1,endosomes
R5382 T8507 T8504 punct ),endosomes
R5383 T8508 T8504 punct ", ",endosomes
R5384 T8509 T8504 cc and,endosomes
R5385 T8510 T8511 amod late,endosomes
R5386 T8511 T8504 conj endosomes,endosomes
R5387 T8512 T8511 punct (,endosomes
R5388 T8513 T8511 appos Rab7,endosomes
R5389 T8514 T8452 punct ),shown
R5390 T8515 T8452 punct .,shown
R5391 T8517 T8518 amod Significant,overlap
R5392 T8518 T8519 nsubjpass overlap,seen
R5393 T8520 T8518 prep of,overlap
R5394 T8521 T8522 compound Batp1,signal
R5395 T8522 T8520 pobj signal,of
R5396 T8523 T8524 punct (,red
R5397 T8524 T8522 parataxis red,signal
R5398 T8525 T8524 punct ),red
R5399 T8526 T8518 prep with,overlap
R5400 T8527 T8526 pobj EEA1,with
R5401 T8528 T8529 punct (,panels
R5402 T8529 T8527 parataxis panels,EEA1
R5403 T8530 T8529 amod green,panels
R5404 T8531 T8529 punct ", ",panels
R5405 T8532 T8529 amod middle,panels
R5406 T8533 T8529 punct ),panels
R5407 T8534 T8527 cc and,EEA1
R5408 T8535 T8527 conj Rab7,EEA1
R5409 T8536 T8537 punct (,panels
R5410 T8537 T8535 parataxis panels,Rab7
R5411 T8538 T8537 amod green,panels
R5412 T8539 T8537 punct ", ",panels
R5413 T8540 T8537 amod bottom,panels
R5414 T8541 T8537 punct ),panels
R5415 T8542 T8519 aux can,seen
R5416 T8543 T8519 auxpass be,seen
R5417 T8544 T8519 prep as,seen
R5418 T8545 T8544 amod yellow,as
R5419 T8546 T8547 advmod when,merged
R5420 T8547 T8519 advcl merged,seen
R5421 T8548 T8549 det the,channels
R5422 T8549 T8547 nsubjpass channels,merged
R5423 T8550 T8549 nummod two,channels
R5424 T8551 T8547 auxpass are,merged
R5425 T8552 T8553 punct (,Merge
R5426 T8553 T8547 parataxis Merge,merged
R5427 T8554 T8553 punct ),Merge
R5428 T8555 T8519 punct .,seen
R5429 T8557 T8558 det The,degree
R5430 T8558 T8559 nsubj degree,is
R5431 T8560 T8558 prep of,degree
R5432 T8561 T8562 compound Batp1,overlap
R5433 T8562 T8560 pobj overlap,of
R5434 T8563 T8559 acomp greatest,is
R5435 T8564 T8559 prep with,is
R5436 T8565 T8564 pobj Rab7,with
R5437 T8566 T8559 punct .,is
R5438 T8568 T8569 advmod Only,overlap
R5439 T8569 T8571 nsubjpass overlap,seen
R5440 T8570 T8569 amod limited,overlap
R5441 T8572 T8569 prep between,overlap
R5442 T8573 T8572 pobj Batp1,between
R5443 T8574 T8575 punct (,red
R5444 T8575 T8573 parataxis red,Batp1
R5445 T8576 T8575 punct ),red
R5446 T8577 T8573 cc and,Batp1
R5447 T8578 T8573 conj Lamp,Batp1
R5448 T8579 T8578 nummod 1,Lamp
R5449 T8580 T8581 punct (,panels
R5450 T8581 T8578 parataxis panels,Lamp
R5451 T8582 T8581 amod green,panels
R5452 T8583 T8581 punct ", ",panels
R5453 T8584 T8581 amod top,panels
R5454 T8585 T8581 punct ),panels
R5455 T8586 T8571 aux can,seen
R5456 T8587 T8571 auxpass be,seen
R5457 T8588 T8571 punct .,seen
R5458 T8590 T8591 compound Batp1,signal
R5459 T8591 T8592 nsubj signal,is
R5460 T8593 T8591 prep in,signal
R5461 T8594 T8595 amod homozygous,cells
R5462 T8595 T8593 pobj cells,in
R5463 T8596 T8595 nmod CbCln3Δex7,cells
R5464 T8597 T8596 punct /,CbCln3Δex7
R5465 T8598 T8596 nummod 8,CbCln3Δex7
R5466 T8599 T8600 advmod significantly,reduced
R5467 T8600 T8592 acomp reduced,is
R5468 T8601 T8592 punct ", ",is
R5469 T8602 T8592 cc but,is
R5470 T8603 T8604 amod significant,overlap
R5471 T8604 T8605 nsubjpass overlap,seen
R5472 T8605 T8592 conj seen,is
R5473 T8606 T8604 prep with,overlap
R5474 T8607 T8606 pobj EEA1,with
R5475 T8608 T8607 cc and,EEA1
R5476 T8609 T8607 conj Rab7,EEA1
R5477 T8610 T8604 punct ", ",overlap
R5478 T8611 T8604 cc and,overlap
R5479 T8612 T8613 advmod very,little
R5480 T8613 T8614 amod little,overlap
R5481 T8614 T8604 conj overlap,overlap
R5482 T8615 T8614 nmod Lamp,overlap
R5483 T8616 T8615 nummod 1,Lamp
R5484 T8617 T8605 punct ", ",seen
R5485 T8618 T8605 aux can,seen
R5486 T8619 T8605 auxpass be,seen
R5487 T8620 T8605 prep as,seen
R5488 T8621 T8620 amod yellow,as
R5489 T8622 T8605 prep in,seen
R5490 T8623 T8624 det the,panels
R5491 T8624 T8622 pobj panels,in
R5492 T8625 T8624 amod respective,panels
R5493 T8626 T8624 amod merged,panels
R5494 T8627 T8605 punct .,seen
R5495 T8629 T8630 advmod Notably,appear
R5496 T8631 T8630 punct ", ",appear
R5497 T8632 T8633 nmod Lamp,localization
R5498 T8633 T8630 nsubj localization,appear
R5499 T8634 T8632 nummod 1,Lamp
R5500 T8635 T8632 cc and,Lamp
R5501 T8636 T8632 conj EEA1,Lamp
R5502 T8637 T8630 oprd altered,appear
R5503 T8638 T8630 punct ", ",appear
R5504 T8639 T8630 cc and,appear
R5505 T8640 T8641 compound Rab7,staining
R5506 T8641 T8642 nsubj staining,was
R5507 T8642 T8630 conj was,appear
R5508 T8643 T8642 advmod frequently,was
R5509 T8644 T8645 advmod less,intense
R5510 T8645 T8642 acomp intense,was
R5511 T8646 T8642 prep in,was
R5512 T8647 T8648 amod homozygous,cells
R5513 T8648 T8646 pobj cells,in
R5514 T8649 T8648 nmod CbCln3Δex7,cells
R5515 T8650 T8649 punct /,CbCln3Δex7
R5516 T8651 T8649 nummod 8,CbCln3Δex7
R5517 T8652 T8642 punct .,was
R5518 T8654 T8655 amod Wild,type
R5519 T8655 T8657 nsubjpass type,captured
R5520 T8656 T8655 punct -,type
R5521 T8658 T8655 cc and,type
R5522 T8659 T8660 amod homozygous,CbCln3Δex7
R5523 T8660 T8661 nmod CbCln3Δex7,images
R5524 T8661 T8655 conj images,type
R5525 T8662 T8660 punct /,CbCln3Δex7
R5526 T8663 T8660 nummod 8,CbCln3Δex7
R5527 T8664 T8661 amod confocal,images
R5528 T8665 T8657 auxpass were,captured
R5529 T8666 T8657 prep with,captured
R5530 T8667 T8668 amod identical,settings
R5531 T8668 T8666 pobj settings,with
R5532 T8669 T8668 compound exposure,settings
R5533 T8670 T8657 punct .,captured
R5534 T8672 T8673 nummod 60,magnification
R5535 T8674 T8672 punct ×,60
R5536 T8675 T8673 punct .,magnification
R5537 T8805 T8806 compound Subunit,c
R5538 T8806 T8807 compound c,accumulation
R5539 T8808 T8807 prep in,accumulation
R5540 T8809 T8810 amod homozygous,cells
R5541 T8810 T8808 pobj cells,in
R5542 T8811 T8810 nmod CbCln3Δex7,cells
R5543 T8812 T8811 punct /,CbCln3Δex7
R5544 T8813 T8811 nummod 8,CbCln3Δex7
R5545 T8814 T8815 amod cerebellar,precursor
R5546 T8815 T8810 compound precursor,cells
R5547 T8817 T8818 meta a,shown
R5548 T8819 T8817 punct .,a
R5549 T8820 T8821 compound Subunit,c
R5550 T8821 T8818 nsubjpass c,shown
R5551 T8822 T8821 appos immunostaining,c
R5552 T8823 T8822 cc and,immunostaining
R5553 T8824 T8822 conj autofluorescence,immunostaining
R5554 T8825 T8821 prep of,c
R5555 T8826 T8827 nummod 7,day
R5556 T8827 T8829 compound day,confluency
R5557 T8828 T8827 punct -,day
R5558 T8829 T8830 npadvmod confluency,aged
R5559 T8830 T8832 amod aged,cells
R5560 T8831 T8830 punct -,aged
R5561 T8832 T8825 pobj cells,of
R5562 T8833 T8834 amod wild,type
R5563 T8834 T8832 nmod type,cells
R5564 T8835 T8834 punct -,type
R5565 T8836 T8834 cc and,type
R5566 T8837 T8834 conj homozygous,type
R5567 T8838 T8832 nmod CbCln3Δex7,cells
R5568 T8839 T8838 punct /,CbCln3Δex7
R5569 T8840 T8838 nummod 8,CbCln3Δex7
R5570 T8841 T8818 auxpass is,shown
R5571 T8842 T8818 punct .,shown
R5572 T8844 T8845 amod Wild,type
R5573 T8845 T8847 compound type,cultures
R5574 T8846 T8845 punct -,type
R5575 T8847 T8848 nsubj cultures,exhibited
R5576 T8849 T8847 punct (,cultures
R5577 T8850 T8847 appos CbCln3,cultures
R5578 T8851 T8850 punct +,CbCln3
R5579 T8852 T8850 punct /,CbCln3
R5580 T8853 T8850 punct +,CbCln3
R5581 T8854 T8848 punct ),exhibited
R5582 T8855 T8856 amod limited,c
R5583 T8856 T8848 dobj c,exhibited
R5584 T8857 T8856 compound subunit,c
R5585 T8858 T8856 appos immunostaining,c
R5586 T8859 T8858 cc and,immunostaining
R5587 T8860 T8858 conj autofluorescence,immunostaining
R5588 T8861 T8848 punct .,exhibited
R5589 T8863 T8864 advmod However,contained
R5590 T8865 T8864 punct ", ",contained
R5591 T8866 T8867 nmod CbCln3Δex7,Δex7
R5592 T8867 T8871 nmod Δex7,cells
R5593 T8868 T8867 punct /,Δex7
R5594 T8869 T8867 nummod 8,Δex7
R5595 T8870 T8867 punct /,Δex7
R5596 T8871 T8864 nsubj cells,contained
R5597 T8872 T8867 punct /,Δex7
R5598 T8873 T8867 nummod 8,Δex7
R5599 T8874 T8875 amod numerous,puncta
R5600 T8875 T8864 dobj puncta,contained
R5601 T8876 T8877 compound subunit,c
R5602 T8877 T8875 compound c,puncta
R5603 T8878 T8864 punct .,contained
R5604 T8880 T8881 nsubj Autofluorescence,was
R5605 T8882 T8883 punct (,AF
R5606 T8883 T8880 parataxis AF,Autofluorescence
R5607 T8884 T8885 nummod 7,days
R5608 T8885 T8883 compound days,AF
R5609 T8886 T8883 punct ),AF
R5610 T8887 T8881 advmod also,was
R5611 T8888 T8889 advmod significantly,elevated
R5612 T8889 T8881 acomp elevated,was
R5613 T8890 T8891 punct (,panels
R5614 T8891 T8889 parataxis panels,elevated
R5615 T8892 T8891 amod right,panels
R5616 T8893 T8891 punct ),panels
R5617 T8894 T8881 punct ", ",was
R5618 T8895 T8896 mark although,observed
R5619 T8896 T8881 advcl observed,was
R5620 T8897 T8898 amod limited,overlap
R5621 T8898 T8896 nsubjpass overlap,observed
R5622 T8899 T8898 prep with,overlap
R5623 T8900 T8901 compound subunit,c
R5624 T8901 T8902 compound c,puncta
R5625 T8902 T8899 pobj puncta,with
R5626 T8903 T8896 auxpass was,observed
R5627 T8904 T8905 punct (,arrows
R5628 T8905 T8896 parataxis arrows,observed
R5629 T8906 T8905 punct ),arrows
R5630 T8907 T8881 punct .,was
R5631 T8909 T8910 nummod 40,magnification
R5632 T8911 T8909 punct ×,40
R5633 T8912 T8910 punct .,magnification
R5634 T8914 T8915 meta b,shown
R5635 T8916 T8914 punct .,b
R5636 T8917 T8918 compound Immunoblot,analysis
R5637 T8918 T8915 nsubjpass analysis,shown
R5638 T8919 T8918 prep of,analysis
R5639 T8920 T8921 compound subunit,c
R5640 T8921 T8922 compound c,protein
R5641 T8922 T8919 pobj protein,of
R5642 T8923 T8918 prep at,analysis
R5643 T8924 T8925 nmod sub-confluency,incubation
R5644 T8925 T8923 pobj incubation,at
R5645 T8926 T8924 cc or,sub-confluency
R5646 T8927 T8928 nummod 7,day
R5647 T8928 T8930 compound day,confluency
R5648 T8929 T8928 punct -,day
R5649 T8930 T8924 conj confluency,sub-confluency
R5650 T8931 T8915 auxpass is,shown
R5651 T8932 T8915 punct .,shown
R5652 T8934 T8935 amod Total,extracts
R5653 T8935 T8937 nsubj extracts,contained
R5654 T8936 T8935 compound protein,extracts
R5655 T8938 T8935 prep from,extracts
R5656 T8939 T8940 nmod sub-confluency,cultures
R5657 T8940 T8938 pobj cultures,from
R5658 T8941 T8942 amod wild,type
R5659 T8942 T8940 nmod type,cultures
R5660 T8943 T8942 punct -,type
R5661 T8944 T8942 punct (,type
R5662 T8945 T8946 punct +,+
R5663 T8946 T8942 appos +,type
R5664 T8947 T8946 punct /,+
R5665 T8948 T8942 punct ),type
R5666 T8949 T8942 cc and,type
R5667 T8950 T8951 amod homozygous,mutant
R5668 T8951 T8942 conj mutant,type
R5669 T8952 T8951 punct (,mutant
R5670 T8953 T8951 appos Δex7,mutant
R5671 T8954 T8953 punct /,Δex7
R5672 T8955 T8953 nummod 8,Δex7
R5673 T8956 T8953 punct /,Δex7
R5674 T8957 T8953 appos Δex7,Δex7
R5675 T8958 T8957 punct /,Δex7
R5676 T8959 T8957 nummod 8,Δex7
R5677 T8960 T8940 punct ),cultures
R5678 T8961 T8962 advmod approximately,equal
R5679 T8962 T8963 amod equal,levels
R5680 T8963 T8937 dobj levels,contained
R5681 T8964 T8963 prep of,levels
R5682 T8965 T8966 compound subunit,c
R5683 T8966 T8967 compound c,protein
R5684 T8967 T8964 pobj protein,of
R5685 T8968 T8967 punct (,protein
R5686 T8969 T8970 nmod α,c
R5687 T8970 T8967 appos c,protein
R5688 T8971 T8969 punct -,α
R5689 T8972 T8969 amod sub,α
R5690 T8973 T8937 punct ),contained
R5691 T8974 T8937 punct .,contained
R5692 T8976 T8977 nummod 7,day
R5693 T8977 T8979 compound day,confluency
R5694 T8978 T8977 punct -,day
R5695 T8979 T8980 compound confluency,extract
R5696 T8980 T8981 nsubj extract,had
R5697 T8982 T8980 prep from,extract
R5698 T8983 T8984 amod homozygous,cells
R5699 T8984 T8982 pobj cells,from
R5700 T8985 T8984 nmod CbCln3Δex7,cells
R5701 T8986 T8985 punct /,CbCln3Δex7
R5702 T8987 T8985 nummod 8,CbCln3Δex7
R5703 T8988 T8984 punct (,cells
R5704 T8989 T8984 appos Δex7,cells
R5705 T8990 T8989 punct /,Δex7
R5706 T8991 T8989 nummod 8,Δex7
R5707 T8992 T8989 punct /,Δex7
R5708 T8993 T8989 appos Δex7,Δex7
R5709 T8994 T8993 punct /,Δex7
R5710 T8995 T8993 nummod 8,Δex7
R5711 T8996 T8981 punct ),had
R5712 T8997 T8998 amod elevated,levels
R5713 T8998 T8981 dobj levels,had
R5714 T8999 T8998 prep of,levels
R5715 T9000 T9001 compound subunit,c
R5716 T9001 T9002 compound c,protein
R5717 T9002 T8999 pobj protein,of
R5718 T9003 T8998 punct (,levels
R5719 T9004 T9005 punct ~,1.5
R5720 T9005 T8998 npadvmod 1.5,levels
R5721 T9006 T9005 punct X,1.5
R5722 T9007 T8998 punct ),levels
R5723 T9008 T8998 punct ", ",levels
R5724 T9009 T8998 amod relative,levels
R5725 T9010 T9009 prep to,relative
R5726 T9011 T9012 amod wild,type
R5727 T9012 T9014 compound type,extract
R5728 T9013 T9012 punct -,type
R5729 T9014 T9010 pobj extract,to
R5730 T9015 T9014 punct (,extract
R5731 T9016 T9017 punct +,+
R5732 T9017 T9014 appos +,extract
R5733 T9018 T9017 punct /,+
R5734 T9019 T8981 punct ),had
R5735 T9020 T8981 punct .,had
R5736 T9022 T9023 compound Protein,levels
R5737 T9023 T9024 nsubjpass levels,normalized
R5738 T9025 T9024 auxpass were,normalized
R5739 T9026 T9024 prep to,normalized
R5740 T9027 T9028 compound cytochrome,c
R5741 T9028 T9029 compound c,oxidase
R5742 T9029 T9030 compound oxidase,subunit
R5743 T9030 T9026 pobj subunit,to
R5744 T9031 T9030 nummod IV,subunit
R5745 T9032 T9030 punct (,subunit
R5746 T9033 T9034 compound α,cox4
R5747 T9034 T9030 appos cox4,subunit
R5748 T9035 T9034 punct -,cox4
R5749 T9036 T9024 punct ),normalized
R5750 T9037 T9024 punct .,normalized
R5751 T9039 T9040 meta c,shown
R5752 T9041 T9039 punct .,c
R5753 T9042 T9043 compound TEM,analysis
R5754 T9043 T9040 nsubjpass analysis,shown
R5755 T9044 T9043 prep of,analysis
R5756 T9045 T9044 pobj inclusions,of
R5757 T9046 T9043 prep in,analysis
R5758 T9047 T9048 nummod 7,day
R5759 T9048 T9050 compound day,confluency
R5760 T9049 T9048 punct -,day
R5761 T9050 T9051 npadvmod confluency,aged
R5762 T9051 T9053 amod aged,cells
R5763 T9052 T9051 punct -,aged
R5764 T9053 T9046 pobj cells,in
R5765 T9054 T9053 amod homozygous,cells
R5766 T9055 T9053 nmod CbCln3Δex7,cells
R5767 T9056 T9055 punct /,CbCln3Δex7
R5768 T9057 T9055 nummod 8,CbCln3Δex7
R5769 T9058 T9040 auxpass is,shown
R5770 T9059 T9040 punct .,shown
R5771 T9061 T9062 det A,autophagosome
R5772 T9062 T9064 nsubjpass autophagosome,filled
R5773 T9063 T9062 amod large,autophagosome
R5774 T9065 T9062 acl contained,autophagosome
R5775 T9066 T9065 agent by,contained
R5776 T9067 T9068 amod double,membrane
R5777 T9068 T9066 pobj membrane,by
R5778 T9069 T9070 punct (,arrows
R5779 T9070 T9065 parataxis arrows,contained
R5780 T9071 T9070 punct ),arrows
R5781 T9072 T9064 auxpass is,filled
R5782 T9073 T9064 prep with,filled
R5783 T9074 T9075 amod degenerating,mitochondria
R5784 T9075 T9073 pobj mitochondria,with
R5785 T9076 T9075 punct (,mitochondria
R5786 T9077 T9075 appos Md,mitochondria
R5787 T9078 T9075 punct ),mitochondria
R5788 T9079 T9075 punct ", ",mitochondria
R5789 T9080 T9081 nmod electron,cores
R5790 T9081 T9075 conj cores,mitochondria
R5791 T9082 T9081 amod dense,cores
R5792 T9083 T9084 punct (,left
R5793 T9084 T9081 parataxis left,cores
R5794 T9085 T9084 cc and,left
R5795 T9086 T9084 conj right,left
R5796 T9087 T9084 prep of,left
R5797 T9088 T9087 punct *,of
R5798 T9089 T9084 punct ),left
R5799 T9090 T9081 cc and,cores
R5800 T9091 T9092 amod other,structures
R5801 T9092 T9081 conj structures,cores
R5802 T9093 T9092 amod smaller,structures
R5803 T9094 T9092 amod vesicular,structures
R5804 T9095 T9064 punct .,filled
R5805 T9097 T9098 det A,inclusion
R5806 T9098 T9103 nsubj inclusion,is
R5807 T9099 T9098 amod large,inclusion
R5808 T9100 T9101 npadvmod electron,dense
R5809 T9101 T9098 amod dense,inclusion
R5810 T9102 T9101 punct -,dense
R5811 T9104 T9098 punct ", ",inclusion
R5812 T9105 T9098 prep with,inclusion
R5813 T9106 T9107 det a,appearance
R5814 T9107 T9105 pobj appearance,with
R5815 T9108 T9107 nmod lipofuscin,appearance
R5816 T9109 T9108 punct (,lipofuscin
R5817 T9110 T9108 appos Ln,lipofuscin
R5818 T9111 T9107 punct ),appearance
R5819 T9112 T9103 punct ", ",is
R5820 T9113 T9103 advmod also,is
R5821 T9114 T9103 acomp present,is
R5822 T9115 T9103 punct .,is
R5823 T9118 T9117 punct ", ",M
R5824 T9119 T9117 appos mitochondria,M
R5825 T9120 T9117 punct .,M
R5826 T9122 T9123 nummod "10,000",magnification
R5827 T9124 T9122 punct ×,"10,000"
R5828 T9125 T9123 punct .,magnification
R5829 T9254 T9255 compound Cathepsin,D
R5830 T9256 T9255 appos localization,D
R5831 T9257 T9256 cc and,localization
R5832 T9258 T9256 conj processing,localization
R5833 T9259 T9255 prep in,D
R5834 T9260 T9261 amod wild,type
R5835 T9261 T9263 nmod type,cells
R5836 T9262 T9261 punct -,type
R5837 T9263 T9259 pobj cells,in
R5838 T9264 T9261 cc and,type
R5839 T9265 T9261 conj homozygous,type
R5840 T9266 T9263 nmod CbCln3Δex7,cells
R5841 T9267 T9266 punct /,CbCln3Δex7
R5842 T9268 T9266 nummod 8,CbCln3Δex7
R5843 T9270 T9271 meta a,shown
R5844 T9272 T9270 punct .,a
R5845 T9273 T9271 nsubjpass Immunostaining,shown
R5846 T9274 T9273 prep of,Immunostaining
R5847 T9275 T9276 amod wild,type
R5848 T9276 T9278 nmod type,cells
R5849 T9277 T9276 punct -,type
R5850 T9278 T9274 pobj cells,of
R5851 T9279 T9276 cc and,type
R5852 T9280 T9276 conj homozygous,type
R5853 T9281 T9278 nmod CbCln3Δex7,cells
R5854 T9282 T9281 punct /,CbCln3Δex7
R5855 T9283 T9281 nummod 8,CbCln3Δex7
R5856 T9284 T9278 compound precursor,cells
R5857 T9285 T9273 prep with,Immunostaining
R5858 T9286 T9287 amod anti-cathepsin,D
R5859 T9287 T9288 amod D,antibody
R5860 T9288 T9285 pobj antibody,with
R5861 T9289 T9273 punct ", ",Immunostaining
R5862 T9290 T9273 acl recognizing,Immunostaining
R5863 T9291 T9292 amod unprocessed,forms
R5864 T9292 T9290 dobj forms,recognizing
R5865 T9293 T9291 cc and,unprocessed
R5866 T9294 T9291 conj processed,unprocessed
R5867 T9295 T9292 prep of,forms
R5868 T9296 T9297 compound cathepsin,D
R5869 T9297 T9298 compound D,protein
R5870 T9298 T9295 pobj protein,of
R5871 T9299 T9271 auxpass is,shown
R5872 T9300 T9271 punct .,shown
R5873 T9302 T9303 nmod CbCln3,cells
R5874 T9303 T9307 nsubj cells,exhibited
R5875 T9304 T9302 punct +,CbCln3
R5876 T9305 T9302 punct /,CbCln3
R5877 T9306 T9302 punct +,CbCln3
R5878 T9308 T9309 punct (,panel
R5879 T9309 T9303 parataxis panel,cells
R5880 T9310 T9309 amod left,panel
R5881 T9311 T9309 punct ),panel
R5882 T9312 T9313 det a,signal
R5883 T9313 T9307 dobj signal,exhibited
R5884 T9314 T9313 amod perinuclear,signal
R5885 T9315 T9314 cc and,perinuclear
R5886 T9316 T9314 conj cytoplasmic,perinuclear
R5887 T9317 T9313 amod punctate,signal
R5888 T9318 T9307 punct .,exhibited
R5889 T9320 T9321 compound Cathepsin,D
R5890 T9321 T9322 compound D,signal
R5891 T9322 T9323 nsubj signal,was
R5892 T9324 T9322 prep in,signal
R5893 T9325 T9326 amod homozygous,cells
R5894 T9326 T9324 pobj cells,in
R5895 T9327 T9326 nmod CbCln3Δex7,cells
R5896 T9328 T9327 punct /,CbCln3Δex7
R5897 T9329 T9327 nummod 8,CbCln3Δex7
R5898 T9330 T9331 punct (,panel
R5899 T9331 T9322 parataxis panel,signal
R5900 T9332 T9331 amod right,panel
R5901 T9333 T9331 punct ),panel
R5902 T9334 T9335 advmod more,often
R5903 T9335 T9323 advmod often,was
R5904 T9336 T9323 acomp perinuclear,was
R5905 T9337 T9323 punct ", ",was
R5906 T9338 T9323 prep with,was
R5907 T9339 T9340 advmod less,signal
R5908 T9340 T9338 pobj signal,with
R5909 T9341 T9342 amod cytoplasmic,punctate
R5910 T9342 T9340 compound punctate,signal
R5911 T9343 T9340 punct ", ",signal
R5912 T9344 T9340 prep compared,signal
R5913 T9345 T9344 prep to,compared
R5914 T9346 T9347 amod wild,type
R5915 T9347 T9349 nmod type,cells
R5916 T9348 T9347 punct -,type
R5917 T9349 T9345 pobj cells,to
R5918 T9350 T9349 nmod CbCln3,cells
R5919 T9351 T9350 punct +,CbCln3
R5920 T9352 T9350 punct /,CbCln3
R5921 T9353 T9350 punct +,CbCln3
R5922 T9354 T9323 punct .,was
R5923 T9356 T9357 nummod 40,magnification
R5924 T9358 T9356 punct ×,40
R5925 T9359 T9357 punct .,magnification
R5926 T9361 T9362 meta b,shown
R5927 T9363 T9361 punct .,b
R5928 T9364 T9365 compound α,D
R5929 T9365 T9368 npadvmod D,probed
R5930 T9366 T9365 punct -,D
R5931 T9367 T9365 compound Cathepsin,D
R5932 T9368 T9370 amod probed,immunoblots
R5933 T9369 T9368 punct -,probed
R5934 T9370 T9362 nsubjpass immunoblots,shown
R5935 T9371 T9370 prep of,immunoblots
R5936 T9372 T9373 amod total,tissue
R5937 T9373 T9371 pobj tissue,of
R5938 T9374 T9375 amod wild,type
R5939 T9375 T9373 nmod type,tissue
R5940 T9376 T9375 punct -,type
R5941 T9377 T9375 cc versus,type
R5942 T9378 T9379 amod homozygous,Cln3Δex7
R5943 T9379 T9380 npadvmod Cln3Δex7,knock
R5944 T9380 T9375 conj knock,type
R5945 T9381 T9379 punct /,Cln3Δex7
R5946 T9382 T9379 nummod 8,Cln3Δex7
R5947 T9383 T9380 punct -,knock
R5948 T9384 T9380 prt in,knock
R5949 T9385 T9373 cc or,tissue
R5950 T9386 T9387 nmod CbCln3Δex7,extracts
R5951 T9387 T9373 conj extracts,tissue
R5952 T9388 T9386 punct /,CbCln3Δex7
R5953 T9389 T9386 nummod 8,CbCln3Δex7
R5954 T9390 T9387 amod cellular,extracts
R5955 T9391 T9362 auxpass are,shown
R5956 T9392 T9362 punct .,shown
R5957 T9394 T9395 det The,band
R5958 T9395 T9401 nsubj band,was
R5959 T9396 T9397 punct ~,45
R5960 T9397 T9398 nummod 45,kDa
R5961 T9398 T9395 compound kDa,band
R5962 T9399 T9400 compound cathepsin,D
R5963 T9400 T9395 compound D,band
R5964 T9402 T9395 punct ", ",band
R5965 T9403 T9395 acl representing,band
R5966 T9404 T9403 dobj precursor,representing
R5967 T9405 T9401 punct ", ",was
R5968 T9406 T9407 det the,band
R5969 T9407 T9401 attr band,was
R5970 T9408 T9407 amod predominant,band
R5971 T9409 T9407 prep in,band
R5972 T9410 T9411 amod wild,type
R5973 T9411 T9409 pobj type,in
R5974 T9412 T9411 punct -,type
R5975 T9413 T9411 punct (,type
R5976 T9414 T9411 appos wt,type
R5977 T9415 T9411 punct ),type
R5978 T9416 T9411 appos tissue,type
R5979 T9417 T9416 cc and,tissue
R5980 T9418 T9419 amod cellular,extracts
R5981 T9419 T9416 conj extracts,tissue
R5982 T9420 T9407 punct ", ",band
R5983 T9421 T9407 prep with,band
R5984 T9422 T9423 amod lower,levels
R5985 T9423 T9421 pobj levels,with
R5986 T9424 T9423 prep of,levels
R5987 T9425 T9426 amod mature,enzyme
R5988 T9426 T9424 pobj enzyme,of
R5989 T9427 T9428 punct (,kDa
R5990 T9428 T9401 parataxis kDa,was
R5991 T9429 T9430 amod single,chain
R5992 T9430 T9428 dep chain,kDa
R5993 T9431 T9428 punct ", ",kDa
R5994 T9432 T9433 punct ~,43
R5995 T9433 T9428 nummod 43,kDa
R5996 T9434 T9428 punct ", ",kDa
R5997 T9435 T9428 cc and,kDa
R5998 T9436 T9437 amod heavy,chain
R5999 T9437 T9438 dep chain,kDa
R6000 T9438 T9428 conj kDa,kDa
R6001 T9439 T9438 punct ", ",kDa
R6002 T9440 T9441 punct ~,31
R6003 T9441 T9438 nummod 31,kDa
R6004 T9442 T9438 punct ),kDa
R6005 T9443 T9401 punct .,was
R6006 T9445 T9446 advmod Conversely,exhibited
R6007 T9447 T9446 punct ", ",exhibited
R6008 T9448 T9449 amod homozygous,extracts
R6009 T9449 T9446 nsubj extracts,exhibited
R6010 T9450 T9449 nmod Cln3Δex7,extracts
R6011 T9451 T9450 punct /,Cln3Δex7
R6012 T9452 T9450 nummod 8,Cln3Δex7
R6013 T9453 T9450 cc and,Cln3Δex7
R6014 T9454 T9450 conj CbCln3Δex7,Cln3Δex7
R6015 T9455 T9454 punct /,CbCln3Δex7
R6016 T9456 T9454 nummod 8,CbCln3Δex7
R6017 T9457 T9449 nmod mutant,extracts
R6018 T9458 T9457 punct (,mutant
R6019 T9459 T9457 appos m,mutant
R6020 T9460 T9449 punct ),extracts
R6021 T9461 T9462 amod reduced,levels
R6022 T9462 T9446 dobj levels,exhibited
R6023 T9463 T9462 prep of,levels
R6024 T9464 T9463 pobj precursor,of
R6025 T9465 T9464 cc and,precursor
R6026 T9466 T9467 amod heavy,chain
R6027 T9467 T9464 conj chain,precursor
R6028 T9468 T9464 prep of,precursor
R6029 T9469 T9470 det the,form
R6030 T9470 T9468 pobj form,of
R6031 T9471 T9472 amod double,chain
R6032 T9472 T9470 compound chain,form
R6033 T9473 T9472 punct -,chain
R6034 T9474 T9470 prep of,form
R6035 T9475 T9476 det the,enzyme
R6036 T9476 T9474 pobj enzyme,of
R6037 T9477 T9446 punct ", ",exhibited
R6038 T9478 T9446 prep with,exhibited
R6039 T9479 T9480 amod elevated,levels
R6040 T9480 T9478 pobj levels,with
R6041 T9481 T9480 prep of,levels
R6042 T9482 T9483 amod single,chain
R6043 T9483 T9485 nmod chain,enzyme
R6044 T9484 T9483 punct -,chain
R6045 T9485 T9481 pobj enzyme,of
R6046 T9486 T9485 amod mature,enzyme
R6047 T9487 T9446 punct .,exhibited
R6050 T9581 T9582 nmod Lysotracker,labeling
R6051 T9582 T9586 dep labeling,shown
R6052 T9583 T9581 cc and,Lysotracker
R6053 T9584 T9581 conj Lamp,Lysotracker
R6054 T9585 T9584 nummod 2,Lamp
R6055 T9587 T9582 prep of,labeling
R6056 T9588 T9589 amod wild,type
R6057 T9589 T9591 nmod type,lysosomes
R6058 T9590 T9589 punct -,type
R6059 T9591 T9587 pobj lysosomes,of
R6060 T9592 T9589 cc and,type
R6061 T9593 T9589 conj homozygous,type
R6062 T9594 T9591 nmod CbCln3Δex7,lysosomes
R6063 T9595 T9594 punct /,CbCln3Δex7
R6064 T9596 T9594 nummod 8,CbCln3Δex7
R6065 T9597 T9598 amod Lysosomal,labeling
R6066 T9598 T9586 nsubjpass labeling,shown
R6067 T9599 T9598 prep of,labeling
R6068 T9600 T9601 amod wild,type
R6069 T9601 T9603 nmod type,cells
R6070 T9602 T9601 punct -,type
R6071 T9603 T9599 pobj cells,of
R6072 T9604 T9601 cc and,type
R6073 T9605 T9606 amod homozygous,CbCln3Δex7
R6074 T9606 T9601 conj CbCln3Δex7,type
R6075 T9607 T9606 punct /,CbCln3Δex7
R6076 T9608 T9606 nummod 8,CbCln3Δex7
R6077 T9609 T9603 compound precursor,cells
R6078 T9610 T9598 prep with,labeling
R6079 T9611 T9612 nmod lysotracker,antibody
R6080 T9612 T9610 pobj antibody,with
R6081 T9613 T9611 cc and,lysotracker
R6082 T9614 T9611 conj Lamp,lysotracker
R6083 T9615 T9614 nummod 2,Lamp
R6084 T9616 T9586 auxpass is,shown
R6085 T9617 T9586 punct .,shown
R6086 T9619 T9620 compound Lysotracker,dye
R6087 T9620 T9621 nsubj dye,labeled
R6088 T9622 T9623 punct (,panels
R6089 T9623 T9620 parataxis panels,dye
R6090 T9624 T9623 amod top,panels
R6091 T9625 T9623 punct ),panels
R6092 T9626 T9627 amod large,lysosomes
R6093 T9627 T9621 dobj lysosomes,labeled
R6094 T9628 T9627 punct ", ",lysosomes
R6095 T9629 T9630 amod perinuclear,clustered
R6096 T9630 T9627 amod clustered,lysosomes
R6097 T9631 T9630 punct -,clustered
R6098 T9632 T9627 cc and,lysosomes
R6099 T9633 T9634 amod scattered,lysosomes
R6100 T9634 T9627 conj lysosomes,lysosomes
R6101 T9635 T9621 prep in,labeled
R6102 T9636 T9637 det the,periphery
R6103 T9637 T9635 pobj periphery,in
R6104 T9638 T9637 prep of,periphery
R6105 T9639 T9640 amod wild,type
R6106 T9640 T9642 compound type,cells
R6107 T9641 T9640 punct -,type
R6108 T9642 T9638 pobj cells,of
R6109 T9643 T9642 punct (,cells
R6110 T9644 T9642 appos CbCln3,cells
R6111 T9645 T9644 punct +,CbCln3
R6112 T9646 T9644 punct /,CbCln3
R6113 T9647 T9644 punct +,CbCln3
R6114 T9648 T9621 punct ),labeled
R6115 T9649 T9621 punct .,labeled
R6116 T9651 T9652 compound Lysotracker,stain
R6117 T9652 T9653 nsubj stain,was
R6118 T9654 T9653 advmod dramatically,was
R6119 T9655 T9653 acomp reduced,was
R6120 T9656 T9653 prep in,was
R6121 T9657 T9658 amod homozygous,cells
R6122 T9658 T9656 pobj cells,in
R6123 T9659 T9658 compound mutant,cells
R6124 T9660 T9658 punct (,cells
R6125 T9661 T9658 appos CbCln3Δex7,cells
R6126 T9662 T9661 punct /,CbCln3Δex7
R6127 T9663 T9661 nummod 8,CbCln3Δex7
R6128 T9664 T9661 punct /,CbCln3Δex7
R6129 T9665 T9661 appos Δex7,CbCln3Δex7
R6130 T9666 T9665 punct /,Δex7
R6131 T9667 T9665 nummod 8,Δex7
R6132 T9668 T9653 punct ),was
R6133 T9669 T9653 punct ", ",was
R6134 T9670 T9653 prep with,was
R6135 T9671 T9672 amod smaller,vesicles
R6136 T9672 T9670 pobj vesicles,with
R6137 T9673 T9672 amod labeled,vesicles
R6138 T9674 T9672 cc and,vesicles
R6139 T9675 T9676 advmod less,apparent
R6140 T9676 T9677 amod apparent,clustering
R6141 T9677 T9672 conj clustering,vesicles
R6142 T9678 T9677 amod perinuclear,clustering
R6143 T9679 T9653 punct .,was
R6144 T9681 T9682 nmod Lamp,immunostaining
R6145 T9682 T9688 nsubj immunostaining,showed
R6146 T9683 T9681 nummod 2,Lamp
R6147 T9684 T9685 punct (,panels
R6148 T9685 T9681 parataxis panels,Lamp
R6149 T9686 T9685 amod bottom,panels
R6150 T9687 T9685 punct ),panels
R6151 T9689 T9688 advmod also,showed
R6152 T9690 T9691 amod reduced,intensity
R6153 T9691 T9688 dobj intensity,showed
R6154 T9692 T9691 compound signal,intensity
R6155 T9693 T9688 prep with,showed
R6156 T9694 T9695 advmod less,clustering
R6157 T9695 T9693 pobj clustering,with
R6158 T9696 T9695 amod perinuclear,clustering
R6159 T9697 T9688 prep in,showed
R6160 T9698 T9699 amod homozygous,cells
R6161 T9699 T9697 pobj cells,in
R6162 T9700 T9699 nmod CbCln3Δex7,cells
R6163 T9701 T9700 punct /,CbCln3Δex7
R6164 T9702 T9700 nummod 8,CbCln3Δex7
R6165 T9703 T9688 punct ", ",showed
R6166 T9704 T9705 mark although,was
R6167 T9705 T9688 advcl was,showed
R6168 T9706 T9707 det the,effect
R6169 T9707 T9705 nsubj effect,was
R6170 T9708 T9709 advmod somewhat,less
R6171 T9709 T9710 advmod less,dramatic
R6172 T9710 T9705 acomp dramatic,was
R6173 T9711 T9710 prep than,dramatic
R6174 T9712 T9711 pobj that,than
R6175 T9713 T9712 acl observed,that
R6176 T9714 T9713 prep with,observed
R6177 T9715 T9716 compound Lysotracker,dye
R6178 T9716 T9714 pobj dye,with
R6179 T9717 T9688 punct .,showed
R6180 T9719 T9720 amod Wild,type
R6181 T9720 T9722 nmod type,images
R6182 T9721 T9720 punct -,type
R6183 T9722 T9729 nsubjpass images,captured
R6184 T9723 T9720 cc and,type
R6185 T9724 T9720 conj homozygous,type
R6186 T9725 T9722 nmod CbCln3Δex7,images
R6187 T9726 T9725 punct /,CbCln3Δex7
R6188 T9727 T9725 nummod 8,CbCln3Δex7
R6189 T9728 T9722 amod confocal,images
R6190 T9730 T9729 auxpass were,captured
R6191 T9731 T9729 prep with,captured
R6192 T9732 T9733 amod identical,settings
R6193 T9733 T9731 pobj settings,with
R6194 T9734 T9733 compound exposure,settings
R6195 T9735 T9729 punct .,captured
R6196 T9737 T9738 nummod 60,magnification
R6197 T9739 T9737 punct ×,60
R6198 T9740 T9738 punct .,magnification
R6199 T9816 T9817 dep Endocytosis,shown
R6200 T9818 T9816 prep in,Endocytosis
R6201 T9819 T9820 amod wild,type
R6202 T9820 T9822 nmod type,cells
R6203 T9821 T9820 punct -,type
R6204 T9822 T9818 pobj cells,in
R6205 T9823 T9820 punct ", ",type
R6206 T9824 T9820 conj heterozygous,type
R6207 T9825 T9824 cc and,heterozygous
R6208 T9826 T9824 conj homozygous,heterozygous
R6209 T9827 T9822 nmod CbCln3Δex7,cells
R6210 T9828 T9827 punct /,CbCln3Δex7
R6211 T9829 T9827 nummod 8,CbCln3Δex7
R6212 T9830 T9831 compound Dextran,FITC
R6213 T9831 T9833 compound FITC,uptake
R6214 T9832 T9831 punct -,FITC
R6215 T9833 T9817 nsubjpass uptake,shown
R6216 T9834 T9833 prep in,uptake
R6217 T9835 T9836 amod wild,type
R6218 T9836 T9838 nmod type,cells
R6219 T9837 T9836 punct -,type
R6220 T9838 T9834 pobj cells,in
R6221 T9839 T9836 punct ", ",type
R6222 T9840 T9836 conj heterozygous,type
R6223 T9841 T9840 cc and,heterozygous
R6224 T9842 T9840 conj homozygous,heterozygous
R6225 T9843 T9838 nmod CbCln3Δex7,cells
R6226 T9844 T9843 punct /,CbCln3Δex7
R6227 T9845 T9843 nummod 8,CbCln3Δex7
R6228 T9846 T9838 compound precursor,cells
R6229 T9847 T9817 auxpass is,shown
R6230 T9848 T9817 punct .,shown
R6231 T9850 T9851 prep In,observed
R6232 T9852 T9853 amod wild,type
R6233 T9853 T9855 nmod type,cells
R6234 T9854 T9853 punct -,type
R6235 T9855 T9850 pobj cells,In
R6236 T9856 T9853 punct (,type
R6237 T9857 T9853 appos CbCln3,type
R6238 T9858 T9857 punct +,CbCln3
R6239 T9859 T9857 punct /,CbCln3
R6240 T9860 T9857 punct +,CbCln3
R6241 T9861 T9862 punct ", ",panel
R6242 T9862 T9853 parataxis panel,type
R6243 T9863 T9862 amod left,panel
R6244 T9864 T9862 punct ),panel
R6245 T9865 T9853 cc and,type
R6246 T9866 T9853 conj heterozygous,type
R6247 T9867 T9868 punct (,panel
R6248 T9868 T9866 parataxis panel,heterozygous
R6249 T9869 T9870 nmod CbCln3,Δex7
R6250 T9870 T9868 dep Δex7,panel
R6251 T9871 T9870 punct +,Δex7
R6252 T9872 T9870 punct /,Δex7
R6253 T9873 T9870 punct /,Δex7
R6254 T9874 T9870 nummod 8,Δex7
R6255 T9875 T9868 punct ", ",panel
R6256 T9876 T9868 amod middle,panel
R6257 T9877 T9868 punct ),panel
R6258 T9878 T9851 punct ", ",observed
R6259 T9879 T9880 compound dextran,FITC
R6260 T9880 T9882 compound FITC,label
R6261 T9881 T9880 punct -,FITC
R6262 T9882 T9851 nsubjpass label,observed
R6263 T9883 T9851 auxpass was,observed
R6264 T9884 T9851 prep in,observed
R6265 T9885 T9886 det a,pattern
R6266 T9886 T9884 pobj pattern,in
R6267 T9887 T9888 amod perinuclear,clustered
R6268 T9888 T9886 amod clustered,pattern
R6269 T9889 T9888 punct -,clustered
R6270 T9890 T9886 amod vesicular,pattern
R6271 T9891 T9851 prep with,observed
R6272 T9892 T9893 amod scattered,vesicles
R6273 T9893 T9891 pobj vesicles,with
R6274 T9894 T9893 amod labeled,vesicles
R6275 T9895 T9896 advmod also,present
R6276 T9896 T9893 relcl present,vesicles
R6277 T9897 T9896 prep in,present
R6278 T9898 T9899 det the,periphery
R6279 T9899 T9897 pobj periphery,in
R6280 T9900 T9851 punct .,observed
R6281 T9902 T9903 prep In,reduced
R6282 T9904 T9902 pobj contrast,In
R6283 T9905 T9903 punct ", ",reduced
R6284 T9906 T9907 compound dextran,FITC
R6285 T9907 T9909 compound FITC,label
R6286 T9908 T9907 punct -,FITC
R6287 T9909 T9903 nsubjpass label,reduced
R6288 T9910 T9909 prep of,label
R6289 T9911 T9912 amod homozygous,mutant
R6290 T9912 T9913 nmod mutant,cells
R6291 T9913 T9910 pobj cells,of
R6292 T9914 T9912 punct (,mutant
R6293 T9915 T9916 nmod CbCln3Δex7,Δex7
R6294 T9916 T9912 appos Δex7,mutant
R6295 T9917 T9916 punct /,Δex7
R6296 T9918 T9916 nummod 8,Δex7
R6297 T9919 T9916 punct /,Δex7
R6298 T9920 T9916 punct /,Δex7
R6299 T9921 T9916 nummod 8,Δex7
R6300 T9922 T9923 punct ", ",panel
R6301 T9923 T9912 parataxis panel,mutant
R6302 T9924 T9923 amod right,panel
R6303 T9925 T9923 punct ),panel
R6304 T9926 T9903 auxpass was,reduced
R6305 T9927 T9903 advmod overall,reduced
R6306 T9928 T9903 cc and,reduced
R6307 T9929 T9903 conj exhibited,reduced
R6308 T9930 T9931 amod smaller,vesicles
R6309 T9931 T9929 dobj vesicles,exhibited
R6310 T9932 T9931 amod stained,vesicles
R6311 T9933 T9931 prep with,vesicles
R6312 T9934 T9935 advmod less,clustering
R6313 T9935 T9933 pobj clustering,with
R6314 T9936 T9935 amod perinuclear,clustering
R6315 T9937 T9903 punct .,reduced
R6316 T9939 T9940 amod Confocal,images
R6317 T9940 T9941 nsubjpass images,captured
R6318 T9942 T9941 auxpass were,captured
R6319 T9943 T9941 prep with,captured
R6320 T9944 T9945 amod identical,settings
R6321 T9945 T9943 pobj settings,with
R6322 T9946 T9945 compound exposure,settings
R6323 T9947 T9941 punct .,captured
R6324 T9949 T9950 nummod 40,magnification
R6325 T9951 T9949 punct ×,40
R6326 T9952 T9950 punct .,magnification
R6327 T10102 T10103 amod Mitochondrial,morphology
R6328 T10104 T10103 cc and,morphology
R6329 T10105 T10103 conj function,morphology
R6330 T10106 T10103 prep in,morphology
R6331 T10107 T10108 amod wild,type
R6332 T10108 T10110 nmod type,cells
R6333 T10109 T10108 punct -,type
R6334 T10110 T10106 pobj cells,in
R6335 T10111 T10108 punct ", ",type
R6336 T10112 T10108 conj heterozygous,type
R6337 T10113 T10112 cc and,heterozygous
R6338 T10114 T10112 conj homozygous,heterozygous
R6339 T10115 T10110 nmod CbCln3Δex7,cells
R6340 T10116 T10115 punct /,CbCln3Δex7
R6341 T10117 T10115 nummod 8,CbCln3Δex7
R6342 T10119 T10120 meta a,shown
R6343 T10121 T10119 punct .,a
R6344 T10122 T10123 amod Confocal,micrographs
R6345 T10123 T10120 nsubjpass micrographs,shown
R6346 T10124 T10122 cc and,Confocal
R6347 T10125 T10122 conj TEM,Confocal
R6348 T10126 T10123 prep of,micrographs
R6349 T10127 T10128 amod wild,type
R6350 T10128 T10130 nmod type,morphology
R6351 T10129 T10128 punct -,type
R6352 T10130 T10126 pobj morphology,of
R6353 T10131 T10128 cc and,type
R6354 T10132 T10128 conj homozygous,type
R6355 T10133 T10130 nmod CbCln3Δex7,morphology
R6356 T10134 T10133 punct /,CbCln3Δex7
R6357 T10135 T10133 nummod 8,CbCln3Δex7
R6358 T10136 T10130 amod mitochondrial,morphology
R6359 T10137 T10120 auxpass are,shown
R6360 T10138 T10120 punct .,shown
R6361 T10140 T10141 nsubj Immunostaining,highlighted
R6362 T10142 T10140 prep with,Immunostaining
R6363 T10143 T10144 det the,marker
R6364 T10144 T10142 pobj marker,with
R6365 T10145 T10144 amod inner,marker
R6366 T10146 T10144 amod mitochondrial,marker
R6367 T10147 T10144 compound membrane,marker
R6368 T10148 T10144 punct ", ",marker
R6369 T10149 T10144 appos grp75,marker
R6370 T10150 T10151 punct (,panels
R6371 T10151 T10140 parataxis panels,Immunostaining
R6372 T10152 T10151 amod top,panels
R6373 T10153 T10151 punct ),panels
R6374 T10154 T10155 amod elongated,mitochondria
R6375 T10155 T10141 dobj mitochondria,highlighted
R6376 T10156 T10141 prep in,highlighted
R6377 T10157 T10158 amod homozygous,cells
R6378 T10158 T10156 pobj cells,in
R6379 T10159 T10158 compound mutant,cells
R6380 T10160 T10158 punct (,cells
R6381 T10161 T10162 nmod CbCln3Δex7,Δex7
R6382 T10162 T10158 appos Δex7,cells
R6383 T10163 T10162 punct /,Δex7
R6384 T10164 T10162 nummod 8,Δex7
R6385 T10165 T10162 punct /,Δex7
R6386 T10166 T10162 punct /,Δex7
R6387 T10167 T10162 nummod 8,Δex7
R6388 T10168 T10141 punct ),highlighted
R6389 T10169 T10141 punct ", ",highlighted
R6390 T10170 T10141 advcl relative,highlighted
R6391 T10171 T10170 prep to,relative
R6392 T10172 T10173 amod wild,type
R6393 T10173 T10175 compound type,mitochondria
R6394 T10174 T10173 punct -,type
R6395 T10175 T10171 pobj mitochondria,to
R6396 T10176 T10175 punct (,mitochondria
R6397 T10177 T10175 appos CbCln3,mitochondria
R6398 T10178 T10177 punct +,CbCln3
R6399 T10179 T10177 punct /,CbCln3
R6400 T10180 T10177 punct +,CbCln3
R6401 T10181 T10170 punct ),relative
R6402 T10182 T10183 punct (,insets
R6403 T10183 T10170 parataxis insets,relative
R6404 T10184 T10183 punct ", ",insets
R6405 T10185 T10186 nsubj zoom,2.75
R6406 T10186 T10183 ccomp 2.75,insets
R6407 T10187 T10186 punct =,2.75
R6408 T10188 T10186 punct x,2.75
R6409 T10189 T10183 punct ),insets
R6410 T10190 T10141 punct .,highlighted
R6411 T10192 T10193 amod Mitochondrial,distribution
R6412 T10193 T10194 nsubjpass distribution,altered
R6413 T10195 T10194 auxpass was,altered
R6414 T10196 T10194 neg not,altered
R6415 T10197 T10194 prep from,altered
R6416 T10198 T10199 det the,pattern
R6417 T10199 T10197 pobj pattern,from
R6418 T10200 T10201 amod wild,type
R6419 T10201 T10199 compound type,pattern
R6420 T10202 T10201 punct -,type
R6421 T10203 T10194 punct .,altered
R6422 T10205 T10206 amod Elongated,mitochondria
R6423 T10206 T10211 nsubjpass mitochondria,observed
R6424 T10207 T10206 amod homozygous,mitochondria
R6425 T10208 T10206 nmod CbCln3Δex7,mitochondria
R6426 T10209 T10208 punct /,CbCln3Δex7
R6427 T10210 T10208 nummod 8,CbCln3Δex7
R6428 T10212 T10211 auxpass were,observed
R6429 T10213 T10211 advmod also,observed
R6430 T10214 T10211 prep by,observed
R6431 T10215 T10216 compound TEM,analysis
R6432 T10216 T10214 pobj analysis,by
R6433 T10217 T10211 punct .,observed
R6434 T10219 T10220 nummod 60,magnification
R6435 T10221 T10219 punct ×,60
R6436 T10222 T10220 punct .,magnification
R6437 T10224 T10225 meta b,shown
R6438 T10226 T10224 punct .,b
R6439 T10227 T10228 amod Cellular,ATP
R6440 T10228 T10229 compound ATP,levels
R6441 T10229 T10225 nsubjpass levels,shown
R6442 T10230 T10229 prep in,levels
R6443 T10231 T10232 amod wild,type
R6444 T10232 T10234 nmod type,cells
R6445 T10233 T10232 punct -,type
R6446 T10234 T10230 pobj cells,in
R6447 T10235 T10232 punct ", ",type
R6448 T10236 T10232 conj heterozygous,type
R6449 T10237 T10236 cc and,heterozygous
R6450 T10238 T10236 conj homozygous,heterozygous
R6451 T10239 T10234 nmod CbCln3Δex7,cells
R6452 T10240 T10239 punct /,CbCln3Δex7
R6453 T10241 T10239 nummod 8,CbCln3Δex7
R6454 T10242 T10234 compound precursor,cells
R6455 T10243 T10225 auxpass are,shown
R6456 T10244 T10225 punct .,shown
R6457 T10246 T10247 amod Wild,type
R6458 T10247 T10249 nmod type,cells
R6459 T10248 T10247 punct -,type
R6460 T10249 T10263 nsubj cells,contained
R6461 T10250 T10251 punct (,bar
R6462 T10251 T10247 parataxis bar,type
R6463 T10252 T10251 amod open,bar
R6464 T10253 T10251 punct ),bar
R6465 T10254 T10247 cc and,type
R6466 T10255 T10247 conj heterozygous,type
R6467 T10256 T10257 punct (,bar
R6468 T10257 T10255 parataxis bar,heterozygous
R6469 T10258 T10257 amod gray,bar
R6470 T10259 T10257 punct ),bar
R6471 T10260 T10249 nmod CbCln3Δex7,cells
R6472 T10261 T10260 punct /,CbCln3Δex7
R6473 T10262 T10260 nummod 8,CbCln3Δex7
R6474 T10264 T10265 punct ~,39
R6475 T10265 T10266 nummod 39,μM
R6476 T10266 T10267 compound μM,ATP
R6477 T10267 T10263 dobj ATP,contained
R6478 T10268 T10263 punct ", ",contained
R6479 T10269 T10270 mark while,contained
R6480 T10270 T10263 advcl contained,contained
R6481 T10271 T10272 amod homozygous,cells
R6482 T10272 T10270 nsubj cells,contained
R6483 T10273 T10272 nmod CbCln3Δex7,cells
R6484 T10274 T10273 punct /,CbCln3Δex7
R6485 T10275 T10273 nummod 8,CbCln3Δex7
R6486 T10276 T10277 punct (,bar
R6487 T10277 T10272 parataxis bar,cells
R6488 T10278 T10277 amod black,bar
R6489 T10279 T10277 punct ),bar
R6490 T10280 T10281 punct ~,1.3
R6491 T10281 T10282 npadvmod 1.3,reduced
R6492 T10282 T10284 amod reduced,levels
R6493 T10283 T10281 advmod fold,1.3
R6494 T10284 T10270 dobj levels,contained
R6495 T10285 T10284 prep of,levels
R6496 T10286 T10285 pobj ATP,of
R6497 T10287 T10284 punct (,levels
R6498 T10288 T10289 punct ~,30
R6499 T10289 T10290 nummod 30,μM
R6500 T10290 T10284 appos μM,levels
R6501 T10291 T10284 punct ),levels
R6502 T10292 T10284 punct ", ",levels
R6503 T10293 T10294 dep which,was
R6504 T10294 T10284 relcl was,levels
R6505 T10295 T10296 advmod statistically,significant
R6506 T10296 T10294 acomp significant,was
R6507 T10297 T10294 prep in,was
R6508 T10298 T10299 det a,test
R6509 T10299 T10297 pobj test,in
R6510 T10300 T10299 compound t,test
R6511 T10301 T10299 punct -,test
R6512 T10302 T10303 punct (,0.0001
R6513 T10303 T10294 parataxis 0.0001,was
R6514 T10304 T10303 nsubj p,0.0001
R6515 T10305 T10303 punct <,0.0001
R6516 T10306 T10303 punct ),0.0001
R6517 T10307 T10263 punct .,contained
R6518 T10309 T10310 amod Wild,type
R6519 T10310 T10312 nmod type,levels
R6520 T10311 T10310 punct -,type
R6521 T10312 T10320 nsubj levels,were
R6522 T10313 T10310 cc and,type
R6523 T10314 T10310 conj heterozygous,type
R6524 T10315 T10316 nmod CbCln3Δex7,8
R6525 T10316 T10312 nmod 8,levels
R6526 T10317 T10316 punct /,8
R6527 T10318 T10319 amod cellular,ATP
R6528 T10319 T10312 compound ATP,levels
R6529 T10321 T10320 neg not,were
R6530 T10322 T10323 advmod statistically,different
R6531 T10323 T10320 acomp different,were
R6532 T10324 T10323 prep from,different
R6533 T10325 T10326 det each,other
R6534 T10326 T10324 pobj other,from
R6535 T10327 T10328 punct (,0.4
R6536 T10328 T10320 parataxis 0.4,were
R6537 T10329 T10328 nsubj p,0.4
R6538 T10330 T10328 punct >,0.4
R6539 T10331 T10328 punct ),0.4
R6540 T10332 T10320 punct .,were
R6541 T10334 T10335 det A,representative
R6542 T10335 T10336 nsubjpass representative,shown
R6543 T10337 T10335 prep of,representative
R6544 T10338 T10339 amod triplicate,experiments
R6545 T10339 T10337 pobj experiments,of
R6546 T10340 T10336 auxpass is,shown
R6547 T10341 T10342 punct (,6
R6548 T10342 T10336 parataxis 6,shown
R6549 T10343 T10342 nsubj n,6
R6550 T10344 T10342 punct =,6
R6551 T10345 T10342 prep in,6
R6552 T10346 T10347 det each,experiment
R6553 T10347 T10345 pobj experiment,in
R6554 T10348 T10342 punct ),6
R6555 T10349 T10336 punct .,shown
R6556 T10351 T10352 meta c,shown
R6557 T10353 T10351 punct .,c
R6558 T10354 T10355 compound Cell,survival
R6559 T10355 T10352 nsubjpass survival,shown
R6560 T10356 T10355 prep following,survival
R6561 T10357 T10358 nummod 24,hour
R6562 T10358 T10360 compound hour,treatment
R6563 T10359 T10358 punct -,hour
R6564 T10360 T10356 pobj treatment,following
R6565 T10361 T10362 compound hydrogen,peroxide
R6566 T10362 T10360 compound peroxide,treatment
R6567 T10363 T10352 auxpass is,shown
R6568 T10364 T10352 punct .,shown
R6569 T10366 T10367 amod Homozygous,cells
R6570 T10367 T10371 nsubj cells,were
R6571 T10368 T10367 nmod CbCln3Δex7,cells
R6572 T10369 T10368 punct /,CbCln3Δex7
R6573 T10370 T10368 nummod 8,CbCln3Δex7
R6574 T10372 T10373 punct ~,2-fold
R6575 T10373 T10374 advmod 2-fold,more
R6576 T10374 T10375 advmod more,sensitive
R6577 T10375 T10371 acomp sensitive,were
R6578 T10376 T10375 prep to,sensitive
R6579 T10377 T10378 amod oxidative,stress
R6580 T10378 T10376 pobj stress,to
R6581 T10379 T10378 prep by,stress
R6582 T10380 T10381 compound hydrogen,peroxide
R6583 T10381 T10382 compound peroxide,treatment
R6584 T10382 T10379 pobj treatment,by
R6585 T10383 T10371 punct .,were
R6586 T10385 T10386 amod Wild,type
R6587 T10386 T10388 nmod type,cells
R6588 T10387 T10386 punct -,type
R6589 T10388 T10400 nsubj cells,exhibited
R6590 T10389 T10390 punct (,circle
R6591 T10390 T10386 parataxis circle,type
R6592 T10391 T10390 punct ),circle
R6593 T10392 T10386 cc and,type
R6594 T10393 T10386 conj heterozygous,type
R6595 T10394 T10395 punct (,triangle
R6596 T10395 T10393 parataxis triangle,heterozygous
R6597 T10396 T10395 punct ),triangle
R6598 T10397 T10388 nmod CbCln3Δex7,cells
R6599 T10398 T10397 punct /,CbCln3Δex7
R6600 T10399 T10397 nummod 8,CbCln3Δex7
R6601 T10401 T10402 punct ~,50
R6602 T10402 T10403 nummod 50,%
R6603 T10403 T10404 compound %,rates
R6604 T10404 T10400 dobj rates,exhibited
R6605 T10405 T10404 compound survival,rates
R6606 T10406 T10404 prep with,rates
R6607 T10407 T10408 quantmod 75,100
R6608 T10408 T10410 nummod 100,μM
R6609 T10409 T10408 punct –,100
R6610 T10410 T10411 compound μM,H2O2
R6611 T10411 T10406 pobj H2O2,with
R6612 T10412 T10400 punct ", ",exhibited
R6613 T10413 T10414 mark whereas,had
R6614 T10414 T10400 advcl had,exhibited
R6615 T10415 T10416 amod homozygous,cells
R6616 T10416 T10414 nsubj cells,had
R6617 T10417 T10416 nmod CbCln3Δex7,cells
R6618 T10418 T10417 punct /,CbCln3Δex7
R6619 T10419 T10417 nummod 8,CbCln3Δex7
R6620 T10420 T10421 punct (,squares
R6621 T10421 T10416 parataxis squares,cells
R6622 T10422 T10421 punct ),squares
R6623 T10423 T10424 det a,rate
R6624 T10424 T10414 dobj rate,had
R6625 T10425 T10426 punct ~,50
R6626 T10426 T10427 nummod 50,%
R6627 T10427 T10424 compound %,rate
R6628 T10428 T10424 compound survival,rate
R6629 T10429 T10424 prep with,rate
R6630 T10430 T10431 nummod 50,μM
R6631 T10431 T10432 compound μM,H2O2
R6632 T10432 T10429 pobj H2O2,with
R6633 T10433 T10400 punct .,exhibited
R6634 T10435 T10436 det A,representative
R6635 T10436 T10437 nsubjpass representative,shown
R6636 T10438 T10436 prep of,representative
R6637 T10439 T10440 amod triplicate,experiments
R6638 T10440 T10438 pobj experiments,of
R6639 T10441 T10437 auxpass is,shown
R6640 T10442 T10443 punct (,4
R6641 T10443 T10437 parataxis 4,shown
R6642 T10444 T10443 nsubj n,4
R6643 T10445 T10443 punct =,4
R6644 T10446 T10443 prep in,4
R6645 T10447 T10448 det each,experiment
R6646 T10448 T10446 pobj experiment,in
R6647 T10449 T10443 punct ),4
R6648 T10450 T10437 punct .,shown
R688 T1272 T1271 prep of,Generation
R689 T1273 T1274 det a,model
R690 T1274 T1272 pobj model,of
R691 T1275 T1276 advmod genetically,precise
R692 T1276 T1274 amod precise,model
R693 T1277 T1274 amod cerebellar,model
R694 T1278 T1274 compound JNCL,model
R695 T1279 T1274 compound cell,model
R696 T1281 T1282 aux To,generate
R697 T1282 T1283 advcl generate,immortalized
R698 T1284 T1285 det a,system
R699 T1285 T1282 dobj system,generate
R700 T1286 T1285 amod precise,system
R701 T1287 T1285 amod genetic,system
R702 T1288 T1285 punct ", ",system
R703 T1289 T1290 npadvmod neuron,derived
R704 T1290 T1285 amod derived,system
R705 T1291 T1290 punct -,derived
R706 T1292 T1293 compound JNCL,culture
R707 T1293 T1285 compound culture,system
R708 T1294 T1293 compound cell,culture
R709 T1295 T1283 punct ", ",immortalized
R710 T1296 T1283 nsubj we,immortalized
R711 T1297 T1298 compound granule,neurons
R712 T1298 T1283 dobj neurons,immortalized
R713 T1299 T1298 acl cultured,neurons
R714 T1300 T1299 prep from,cultured
R715 T1301 T1302 amod postnatal,day
R716 T1302 T1303 nmod day,cerebella
R717 T1303 T1300 pobj cerebella,from
R718 T1304 T1302 nummod 4,day
R719 T1305 T1302 punct (,day
R720 T1306 T1302 appos P4,day
R721 T1307 T1303 punct ),cerebella
R722 T1308 T1303 prep of,cerebella
R723 T1309 T1310 amod homozygous,mice
R724 T1310 T1308 pobj mice,of
R725 T1311 T1309 cc and,homozygous
R726 T1312 T1309 conj heterozygous,homozygous
R727 T1313 T1310 nmod Cln3Δex7,mice
R728 T1314 T1313 punct /,Cln3Δex7
R729 T1315 T1313 nummod 8,Cln3Δex7
R730 T1316 T1310 amod knock,mice
R731 T1317 T1316 punct -,knock
R732 T1318 T1316 prt in,knock
R733 T1319 T1310 punct ", ",mice
R734 T1320 T1310 cc and,mice
R735 T1321 T1322 amod wild,type
R736 T1322 T1324 compound type,littermates
R737 T1323 T1322 punct -,type
R738 T1324 T1310 conj littermates,mice
R739 T1325 T1283 punct .,immortalized
R740 T1327 T1328 amod Primary,cultures
R741 T1328 T1330 nsubjpass cultures,transduced
R742 T1329 T1328 compound cell,cultures
R743 T1331 T1328 acl enriched,cultures
R744 T1332 T1331 prep for,enriched
R745 T1333 T1334 compound granule,neurons
R746 T1334 T1332 pobj neurons,for
R747 T1335 T1330 auxpass were,transduced
R748 T1336 T1330 prep with,transduced
R749 T1337 T1338 amod retroviral,vector
R750 T1338 T1336 pobj vector,with
R751 T1339 T1338 acl bearing,vector
R752 T1340 T1341 det a,cassette
R753 T1341 T1339 dobj cassette,bearing
R754 T1342 T1341 compound selection,cassette
R755 T1343 T1341 cc and,cassette
R756 T1344 T1345 npadvmod temperature,sensitive
R757 T1345 T1347 amod sensitive,antigen
R758 T1346 T1345 punct -,sensitive
R759 T1347 T1341 conj antigen,cassette
R760 T1348 T1349 nmod tsA58,SV40
R761 T1349 T1347 nmod SV40,antigen
R762 T1350 T1347 amod large,antigen
R763 T1351 T1347 compound T,antigen
R764 T1352 T1330 punct .,transduced
R765 T1354 T1355 nsubj Growth,allowed
R766 T1356 T1354 prep in,Growth
R767 T1357 T1358 npadvmod G418,containing
R768 T1358 T1359 amod containing,medium
R769 T1359 T1356 pobj medium,in
R770 T1360 T1354 prep at,Growth
R771 T1361 T1362 det the,temperature
R772 T1362 T1360 pobj temperature,at
R773 T1363 T1362 amod permissive,temperature
R774 T1364 T1362 punct (,temperature
R775 T1365 T1366 nummod 33,°C
R776 T1366 T1362 appos °C,temperature
R777 T1367 T1355 punct ),allowed
R778 T1368 T1355 prep for,allowed
R779 T1369 T1368 pobj selection,for
R780 T1370 T1369 cc and,selection
R781 T1371 T1369 conj isolation,selection
R782 T1372 T1369 prep of,selection
R783 T1373 T1374 amod multiple,lines
R784 T1374 T1372 pobj lines,of
R785 T1375 T1376 amod clonal,nestin
R786 T1376 T1377 npadvmod nestin,positive
R787 T1377 T1374 amod positive,lines
R788 T1378 T1377 punct -,positive
R789 T1379 T1380 punct (,1a
R790 T1380 T1377 parataxis 1a,positive
R791 T1381 T1380 compound Fig.,1a
R792 T1382 T1380 punct ),1a
R793 T1383 T1377 punct ", ",positive
R794 T1384 T1377 cc and,positive
R795 T1385 T1386 npadvmod GFAP,negative
R796 T1386 T1377 conj negative,positive
R797 T1387 T1386 punct -,negative
R798 T1388 T1389 punct (,1b
R799 T1389 T1386 parataxis 1b,negative
R800 T1390 T1389 compound Fig.,1b
R801 T1391 T1389 punct ),1b
R802 T1392 T1374 punct ", ",lines
R803 T1393 T1374 compound cell,lines
R804 T1394 T1374 prep for,lines
R805 T1395 T1396 det each,genotype
R806 T1396 T1394 pobj genotype,for
R807 T1397 T1355 punct .,allowed
R808 T1399 T1400 det No,differences
R809 T1400 T1403 nsubjpass differences,observed
R810 T1401 T1400 nmod genotype,differences
R811 T1402 T1400 amod specific,differences
R812 T1404 T1403 auxpass were,observed
R813 T1405 T1403 prep in,observed
R814 T1406 T1407 amod cellular,morphology
R815 T1407 T1405 pobj morphology,in
R816 T1408 T1407 cc or,morphology
R817 T1409 T1410 compound doubling,time
R818 T1410 T1407 conj time,morphology
R819 T1411 T1410 punct (,time
R820 T1412 T1413 punct ~,46
R821 T1413 T1414 nummod 46,hours
R822 T1414 T1410 appos hours,time
R823 T1415 T1403 punct ),observed
R824 T1416 T1417 punct (,shown
R825 T1417 T1403 parataxis shown,observed
R826 T1418 T1417 nsubj data,shown
R827 T1419 T1417 neg not,shown
R828 T1420 T1417 punct ),shown
R829 T1421 T1403 punct .,observed
R830 T1423 T1424 mark As,expected
R831 T1424 T1425 advcl expected,lost
R832 T1426 T1425 punct ", ",lost
R833 T1427 T1428 nmod SV40,expression
R834 T1428 T1425 nsubjpass expression,lost
R835 T1429 T1428 amod large,expression
R836 T1430 T1431 compound T,antigen
R837 T1431 T1428 compound antigen,expression
R838 T1432 T1425 auxpass was,lost
R839 T1433 T1425 advmod rapidly,lost
R840 T1434 T1425 cc and,lost
R841 T1435 T1436 compound cell,division
R842 T1436 T1437 nsubj division,ceased
R843 T1437 T1425 conj ceased,lost
R844 T1438 T1439 advmod when,shifted
R845 T1439 T1437 advcl shifted,ceased
R846 T1440 T1439 nsubjpass cells,shifted
R847 T1441 T1439 auxpass were,shifted
R848 T1442 T1439 prep to,shifted
R849 T1443 T1444 det the,temperature
R850 T1444 T1442 pobj temperature,to
R851 T1445 T1444 amod non-permissive,temperature
R852 T1446 T1444 punct (,temperature
R853 T1447 T1448 nummod 39,°C
R854 T1448 T1444 appos °C,temperature
R855 T1449 T1439 punct ),shifted
R856 T1450 T1451 punct (,shown
R857 T1451 T1439 parataxis shown,shifted
R858 T1452 T1451 nsubj data,shown
R859 T1453 T1451 neg not,shown
R860 T1454 T1451 punct ),shown
R861 T1455 T1437 punct .,ceased
R862 T1457 T1458 prep Upon,became
R863 T1459 T1457 pobj addition,Upon
R864 T1460 T1459 prep of,addition
R865 T1461 T1462 amod neuronal,differentiation
R866 T1462 T1463 compound differentiation,cocktail
R867 T1463 T1460 pobj cocktail,of
R868 T1464 T1458 punct ", ",became
R869 T1465 T1466 compound precursor,cells
R870 T1466 T1458 nsubj cells,became
R871 T1467 T1468 npadvmod neuron,like
R872 T1468 T1458 acomp like,became
R873 T1469 T1468 punct -,like
R874 T1470 T1458 prep in,became
R875 T1471 T1470 pobj morphology,in
R876 T1472 T1458 cc and,became
R877 T1473 T1458 conj exhibited,became
R878 T1474 T1475 amod decreased,expression
R879 T1475 T1473 dobj expression,exhibited
R880 T1476 T1475 compound nestin,expression
R881 T1477 T1478 punct (,shown
R882 T1478 T1475 parataxis shown,expression
R883 T1479 T1478 nsubj data,shown
R884 T1480 T1478 neg not,shown
R885 T1481 T1478 punct ),shown
R886 T1482 T1475 cc and,expression
R887 T1483 T1484 amod increased,expression
R888 T1484 T1475 conj expression,expression
R889 T1485 T1484 nmod MAP2,expression
R890 T1486 T1485 cc and,MAP2
R891 T1487 T1485 conj NeuN,MAP2
R892 T1488 T1489 punct (,1d
R893 T1489 T1484 parataxis 1d,expression
R894 T1490 T1489 nmod Fig.,1d
R895 T1491 T1489 nmod 1c,1d
R896 T1492 T1489 punct ",",1d
R897 T1493 T1489 punct ),1d
R898 T1494 T1475 punct ", ",expression
R899 T1495 T1475 cc but,expression
R900 T1496 T1495 neg not,but
R901 T1497 T1475 conj expression,expression
R902 T1498 T1497 prep of,expression
R903 T1499 T1500 det the,marker
R904 T1500 T1498 pobj marker,of
R905 T1501 T1500 compound Purkinje,marker
R906 T1502 T1500 punct ", ",marker
R907 T1503 T1500 appos calbindin,marker
R908 T1504 T1505 punct (,1e
R909 T1505 T1497 parataxis 1e,expression
R910 T1506 T1505 compound Fig.,1e
R911 T1507 T1505 punct ),1e
R912 T1508 T1458 punct .,became
R913 T1775 T1776 amod Homozygous,cells
R914 T1776 T1780 nsubj cells,express
R915 T1777 T1776 nmod CbCln3Δex7,cells
R916 T1778 T1777 punct /,CbCln3Δex7
R917 T1779 T1777 nummod 8,CbCln3Δex7
R918 T1781 T1782 compound mutant,battenin
R919 T1782 T1780 dobj battenin,express
R920 T1783 T1780 cc and,express
R921 T1784 T1780 conj display,express
R922 T1785 T1786 npadvmod JNCL,like
R923 T1786 T1788 amod like,pathology
R924 T1787 T1786 punct -,like
R925 T1788 T1784 dobj pathology,display
R926 T1790 T1791 amod Homozygous,cells
R927 T1791 T1796 nsubjpass cells,examined
R928 T1792 T1793 nmod Cb,Cln3Δex7
R929 T1793 T1791 nmod Cln3Δex7,cells
R930 T1794 T1793 punct /,Cln3Δex7
R931 T1795 T1793 nummod 8,Cln3Δex7
R932 T1797 T1796 auxpass were,examined
R933 T1798 T1796 advmod first,examined
R934 T1799 T1796 prep for,examined
R935 T1800 T1801 npadvmod JNCL,like
R936 T1801 T1803 amod like,characteristics
R937 T1802 T1801 punct -,like
R938 T1803 T1799 pobj characteristics,for
R939 T1804 T1796 punct .,examined
R940 T1806 T1807 amod Homozygous,mice
R941 T1807 T1814 nsubj mice,express
R942 T1808 T1807 nmod Cln3Δex7,mice
R943 T1809 T1808 punct /,Cln3Δex7
R944 T1810 T1808 nummod 8,Cln3Δex7
R945 T1811 T1807 amod knock,mice
R946 T1812 T1811 punct -,knock
R947 T1813 T1811 prt in,knock
R948 T1815 T1816 amod multiple,variants
R949 T1816 T1814 dobj variants,express
R950 T1817 T1818 compound Cln3,splice
R951 T1818 T1816 compound splice,variants
R952 T1819 T1818 compound mRNA,splice
R953 T1820 T1816 cc and,variants
R954 T1821 T1822 compound mutant,protein
R955 T1822 T1816 conj protein,variants
R956 T1823 T1822 compound battenin,protein
R957 T1824 T1825 dep that,is
R958 T1825 T1822 relcl is,protein
R959 T1826 T1825 acomp detectable,is
R960 T1827 T1825 prep by,is
R961 T1828 T1829 compound batp1,antibody
R962 T1829 T1830 npadvmod antibody,recognizing
R963 T1830 T1831 amod recognizing,epitopes
R964 T1831 T1827 pobj epitopes,by
R965 T1832 T1833 npadvmod C,terminal
R966 T1833 T1831 amod terminal,epitopes
R967 T1834 T1833 punct -,terminal
R968 T1835 T1836 punct [,12
R969 T1836 T1814 parataxis 12,express
R970 T1837 T1836 punct ],12
R971 T1838 T1814 punct .,express
R972 T1840 T1841 aux To,assess
R973 T1841 T1842 advcl assess,performed
R974 T1843 T1844 det this,phenotype
R975 T1844 T1841 dobj phenotype,assess
R976 T1845 T1844 amod molecular,phenotype
R977 T1846 T1841 prep in,assess
R978 T1847 T1848 nmod CbCln3Δex7,cells
R979 T1848 T1846 pobj cells,in
R980 T1849 T1847 punct /,CbCln3Δex7
R981 T1850 T1847 nummod 8,CbCln3Δex7
R982 T1851 T1842 punct ", ",performed
R983 T1852 T1853 compound RT,PCR
R984 T1853 T1842 nsubjpass PCR,performed
R985 T1854 T1853 punct -,PCR
R986 T1855 T1853 cc and,PCR
R987 T1856 T1857 nmod anti-battenin,immunostaining
R988 T1857 T1853 conj immunostaining,PCR
R989 T1858 T1856 punct (,anti-battenin
R990 T1859 T1856 appos batp1,anti-battenin
R991 T1860 T1857 punct ),immunostaining
R992 T1861 T1842 auxpass were,performed
R993 T1862 T1842 punct .,performed
R994 T1864 T1865 mark As,shown
R995 T1865 T1866 advcl shown,were
R996 T1867 T1865 prep in,shown
R997 T1868 T1867 pobj Figure,in
R998 T1869 T1868 nummod 2,Figure
R999 T1870 T1866 punct ", ",were