| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T6925 |
0-5 |
JJ |
denotes |
Total |
| T6926 |
6-14 |
NN |
denotes |
proteins |
| T6928 |
15-19 |
VBD |
denotes |
were |
| T6927 |
20-28 |
VBN |
denotes |
isolated |
| T6929 |
29-33 |
IN |
denotes |
from |
| T6930 |
34-38 |
NN |
denotes |
cell |
| T6931 |
39-46 |
NNS |
denotes |
pellets |
| T6932 |
47-49 |
IN |
denotes |
by |
| T6933 |
50-60 |
NN |
denotes |
extraction |
| T6934 |
61-65 |
IN |
denotes |
with |
| T6935 |
66-69 |
NN |
denotes |
ice |
| T6937 |
69-70 |
HYPH |
denotes |
- |
| T6938 |
70-74 |
JJ |
denotes |
cold |
| T6939 |
75-77 |
CD |
denotes |
20 |
| T6940 |
78-80 |
NN |
denotes |
mM |
| T6936 |
81-85 |
NN |
denotes |
Tris |
| T6941 |
85-87 |
, |
denotes |
, |
| T6942 |
87-89 |
NN |
denotes |
pH |
| T6943 |
90-93 |
CD |
denotes |
7.4 |
| T6944 |
93-95 |
, |
denotes |
, |
| T6945 |
95-96 |
CD |
denotes |
1 |
| T6946 |
96-97 |
NN |
denotes |
% |
| T6948 |
98-104 |
NN |
denotes |
Triton |
| T6947 |
105-106 |
NN |
denotes |
X |
| T6949 |
106-107 |
HYPH |
denotes |
- |
| T6950 |
107-110 |
CD |
denotes |
100 |
| T6951 |
111-112 |
-LRB- |
denotes |
( |
| T6953 |
112-120 |
NN |
denotes |
membrane |
| T6955 |
120-121 |
HYPH |
denotes |
- |
| T6954 |
121-129 |
NN |
denotes |
research |
| T6956 |
130-135 |
NN |
denotes |
grade |
| T6957 |
135-137 |
, |
denotes |
, |
| T6952 |
137-142 |
NNP |
denotes |
Roche |
| T6958 |
142-143 |
-RRB- |
denotes |
) |
| T6959 |
143-145 |
, |
denotes |
, |
| T6960 |
145-149 |
CC |
denotes |
plus |
| T6961 |
150-158 |
NN |
denotes |
protease |
| T6962 |
159-169 |
NNS |
denotes |
inhibitors |
| T6963 |
170-171 |
-LRB- |
denotes |
( |
| T6964 |
171-179 |
JJ |
denotes |
Complete |
| T6966 |
180-184 |
NN |
denotes |
mini |
| T6965 |
185-191 |
NN |
denotes |
tablet |
| T6967 |
191-193 |
, |
denotes |
, |
| T6968 |
193-196 |
CD |
denotes |
0.7 |
| T6969 |
197-199 |
NN |
denotes |
μg |
| T6971 |
199-200 |
SYM |
denotes |
/ |
| T6972 |
200-202 |
NN |
denotes |
ml |
| T6973 |
203-212 |
NN |
denotes |
pepstatin |
| T6970 |
213-214 |
NN |
denotes |
A |
| T6974 |
214-216 |
, |
denotes |
, |
| T6975 |
216-217 |
CD |
denotes |
2 |
| T6976 |
218-220 |
NN |
denotes |
μg |
| T6978 |
220-221 |
SYM |
denotes |
/ |
| T6979 |
221-223 |
NN |
denotes |
ml |
| T6977 |
224-233 |
NN |
denotes |
aprotinin |
| T6980 |
233-235 |
, |
denotes |
, |
| T6981 |
235-236 |
CD |
denotes |
5 |
| T6982 |
237-239 |
NN |
denotes |
μg |
| T6984 |
239-240 |
SYM |
denotes |
/ |
| T6985 |
240-242 |
NN |
denotes |
ml |
| T6983 |
243-252 |
NN |
denotes |
leupeptin |
| T6986 |
253-254 |
-LRB- |
denotes |
[ |
| T6987 |
254-259 |
NNP |
denotes |
Roche |
| T6988 |
259-260 |
-RRB- |
denotes |
] |
| T6989 |
260-261 |
-RRB- |
denotes |
) |
| T6990 |
261-262 |
. |
denotes |
. |
| T6991 |
262-407 |
sentence |
denotes |
Following homogenization through a 25-gauge needle (~10 passes), extracts were centrifuged at 1000 × g for 10 minutes, at 4°C, to remove debris. |
| T6992 |
263-272 |
VBG |
denotes |
Following |
| T6994 |
273-287 |
NN |
denotes |
homogenization |
| T6995 |
288-295 |
IN |
denotes |
through |
| T6996 |
296-297 |
DT |
denotes |
a |
| T6998 |
298-300 |
CD |
denotes |
25 |
| T7000 |
300-301 |
HYPH |
denotes |
- |
| T6999 |
301-306 |
NN |
denotes |
gauge |
| T6997 |
307-313 |
NN |
denotes |
needle |
| T7001 |
314-315 |
-LRB- |
denotes |
( |
| T7003 |
315-316 |
SYM |
denotes |
~ |
| T7004 |
316-318 |
CD |
denotes |
10 |
| T7002 |
319-325 |
NNS |
denotes |
passes |
| T7005 |
325-326 |
-RRB- |
denotes |
) |
| T7006 |
326-328 |
, |
denotes |
, |
| T7007 |
328-336 |
NNS |
denotes |
extracts |
| T7008 |
337-341 |
VBD |
denotes |
were |
| T6993 |
342-353 |
VBN |
denotes |
centrifuged |
| T7009 |
354-356 |
IN |
denotes |
at |
| T7010 |
357-361 |
CD |
denotes |
1000 |
| T7012 |
362-363 |
SYM |
denotes |
× |
| T7011 |
364-365 |
NN |
denotes |
g |
| T7013 |
366-369 |
IN |
denotes |
for |
| T7014 |
370-372 |
CD |
denotes |
10 |
| T7015 |
373-380 |
NNS |
denotes |
minutes |
| T7016 |
380-382 |
, |
denotes |
, |
| T7017 |
382-384 |
IN |
denotes |
at |
| T7018 |
385-386 |
CD |
denotes |
4 |
| T7019 |
386-388 |
NN |
denotes |
°C |
| T7020 |
388-390 |
, |
denotes |
, |
| T7021 |
390-392 |
TO |
denotes |
to |
| T7022 |
393-399 |
VB |
denotes |
remove |
| T7023 |
400-406 |
NN |
denotes |
debris |
| T7024 |
406-407 |
. |
denotes |
. |
| T7025 |
407-556 |
sentence |
denotes |
Typically, 20–40 μg of protein (determined by Bio-rad Dc Protein Assay) was separated by SDS-PAGE, for subsequent immunoblotting, as described [12]. |
| T7026 |
408-417 |
RB |
denotes |
Typically |
| T7028 |
417-419 |
, |
denotes |
, |
| T7029 |
419-421 |
CD |
denotes |
20 |
| T7031 |
421-422 |
SYM |
denotes |
– |
| T7030 |
422-424 |
CD |
denotes |
40 |
| T7032 |
425-427 |
NN |
denotes |
μg |
| T7033 |
428-430 |
IN |
denotes |
of |
| T7034 |
431-438 |
NN |
denotes |
protein |
| T7035 |
439-440 |
-LRB- |
denotes |
( |
| T7036 |
440-450 |
VBN |
denotes |
determined |
| T7037 |
451-453 |
IN |
denotes |
by |
| T7038 |
454-457 |
NNP |
denotes |
Bio |
| T7040 |
457-458 |
HYPH |
denotes |
- |
| T7039 |
458-461 |
NNP |
denotes |
rad |
| T7042 |
462-464 |
NN |
denotes |
Dc |
| T7043 |
465-472 |
NN |
denotes |
Protein |
| T7041 |
473-478 |
NN |
denotes |
Assay |
| T7044 |
478-479 |
-RRB- |
denotes |
) |
| T7045 |
480-483 |
VBD |
denotes |
was |
| T7027 |
484-493 |
VBN |
denotes |
separated |
| T7046 |
494-496 |
IN |
denotes |
by |
| T7047 |
497-500 |
NN |
denotes |
SDS |
| T7049 |
500-501 |
HYPH |
denotes |
- |
| T7048 |
501-505 |
NN |
denotes |
PAGE |
| T7050 |
505-507 |
, |
denotes |
, |
| T7051 |
507-510 |
IN |
denotes |
for |
| T7052 |
511-521 |
JJ |
denotes |
subsequent |
| T7053 |
522-536 |
NN |
denotes |
immunoblotting |
| T7054 |
536-538 |
, |
denotes |
, |
| T7055 |
538-540 |
IN |
denotes |
as |
| T7056 |
541-550 |
VBN |
denotes |
described |
| T7057 |
551-552 |
-LRB- |
denotes |
[ |
| T7058 |
552-554 |
CD |
denotes |
12 |
| T7059 |
554-555 |
-RRB- |
denotes |
] |
| T7060 |
555-556 |
. |
denotes |
. |
| T7061 |
556-641 |
sentence |
denotes |
16.5% tris-tricine SDS-PAGE gels were used for subunit c immunoblotting experiments. |
| T7062 |
557-561 |
CD |
denotes |
16.5 |
| T7063 |
561-562 |
NN |
denotes |
% |
| T7065 |
563-567 |
NN |
denotes |
tris |
| T7067 |
567-568 |
HYPH |
denotes |
- |
| T7066 |
568-575 |
NN |
denotes |
tricine |
| T7068 |
576-579 |
NN |
denotes |
SDS |
| T7070 |
579-580 |
HYPH |
denotes |
- |
| T7069 |
580-584 |
NN |
denotes |
PAGE |
| T7064 |
585-589 |
NNS |
denotes |
gels |
| T7072 |
590-594 |
VBD |
denotes |
were |
| T7071 |
595-599 |
VBN |
denotes |
used |
| T7073 |
600-603 |
IN |
denotes |
for |
| T7074 |
604-611 |
NN |
denotes |
subunit |
| T7075 |
612-613 |
NN |
denotes |
c |
| T7077 |
614-628 |
NN |
denotes |
immunoblotting |
| T7076 |
629-640 |
NNS |
denotes |
experiments |
| T7078 |
640-641 |
. |
denotes |
. |
| R4303 |
T6925 |
T6926 |
amod |
Total,proteins |
| R4304 |
T6926 |
T6927 |
nsubjpass |
proteins,isolated |
| R4305 |
T6928 |
T6927 |
auxpass |
were,isolated |
| R4306 |
T6929 |
T6927 |
prep |
from,isolated |
| R4307 |
T6930 |
T6931 |
compound |
cell,pellets |
| R4308 |
T6931 |
T6929 |
pobj |
pellets,from |
| R4309 |
T6932 |
T6927 |
prep |
by,isolated |
| R4310 |
T6933 |
T6932 |
pobj |
extraction,by |
| R4311 |
T6934 |
T6933 |
prep |
with,extraction |
| R4312 |
T6935 |
T6936 |
nmod |
ice,Tris |
| R4313 |
T6936 |
T6934 |
pobj |
Tris,with |
| R4314 |
T6937 |
T6935 |
punct |
-,ice |
| R4315 |
T6938 |
T6935 |
amod |
cold,ice |
| R4316 |
T6939 |
T6940 |
nummod |
20,mM |
| R4317 |
T6940 |
T6936 |
compound |
mM,Tris |
| R4318 |
T6941 |
T6936 |
punct |
", ",Tris |
| R4319 |
T6942 |
T6936 |
npadvmod |
pH,Tris |
| R4320 |
T6943 |
T6942 |
nummod |
7.4,pH |
| R4321 |
T6944 |
T6936 |
punct |
", ",Tris |
| R4322 |
T6945 |
T6946 |
nummod |
1,% |
| R4323 |
T6946 |
T6947 |
compound |
%,X |
| R4324 |
T6947 |
T6936 |
conj |
X,Tris |
| R4325 |
T6948 |
T6947 |
compound |
Triton,X |
| R4326 |
T6949 |
T6947 |
punct |
-,X |
| R4327 |
T6950 |
T6947 |
nummod |
100,X |
| R4328 |
T6951 |
T6952 |
punct |
(,Roche |
| R4329 |
T6952 |
T6947 |
parataxis |
Roche,X |
| R4330 |
T6953 |
T6954 |
compound |
membrane,research |
| R4331 |
T6954 |
T6956 |
compound |
research,grade |
| R4332 |
T6955 |
T6954 |
punct |
-,research |
| R4333 |
T6956 |
T6952 |
dep |
grade,Roche |
| R4334 |
T6957 |
T6952 |
punct |
", ",Roche |
| R4335 |
T6958 |
T6952 |
punct |
),Roche |
| R4336 |
T6959 |
T6947 |
punct |
", ",X |
| R4337 |
T6960 |
T6947 |
cc |
plus,X |
| R4338 |
T6961 |
T6962 |
compound |
protease,inhibitors |
| R4339 |
T6962 |
T6947 |
conj |
inhibitors,X |
| R4340 |
T6963 |
T6962 |
punct |
(,inhibitors |
| R4341 |
T6964 |
T6965 |
amod |
Complete,tablet |
| R4342 |
T6965 |
T6962 |
appos |
tablet,inhibitors |
| R4343 |
T6966 |
T6965 |
compound |
mini,tablet |
| R4344 |
T6967 |
T6965 |
punct |
", ",tablet |
| R4345 |
T6968 |
T6969 |
nummod |
0.7,μg |
| R4346 |
T6969 |
T6970 |
nmod |
μg,A |
| R4347 |
T6970 |
T6965 |
appos |
A,tablet |
| R4348 |
T6971 |
T6972 |
punct |
/,ml |
| R4349 |
T6972 |
T6969 |
prep |
ml,μg |
| R4350 |
T6973 |
T6970 |
compound |
pepstatin,A |
| R4351 |
T6974 |
T6965 |
punct |
", ",tablet |
| R4352 |
T6975 |
T6976 |
nummod |
2,μg |
| R4353 |
T6976 |
T6977 |
nmod |
μg,aprotinin |
| R4354 |
T6977 |
T6965 |
appos |
aprotinin,tablet |
| R4355 |
T6978 |
T6979 |
punct |
/,ml |
| R4356 |
T6979 |
T6976 |
prep |
ml,μg |
| R4357 |
T6980 |
T6965 |
punct |
", ",tablet |
| R4358 |
T6981 |
T6982 |
nummod |
5,μg |
| R4359 |
T6982 |
T6983 |
nmod |
μg,leupeptin |
| R4360 |
T6983 |
T6965 |
appos |
leupeptin,tablet |
| R4361 |
T6984 |
T6985 |
punct |
/,ml |
| R4362 |
T6985 |
T6982 |
prep |
ml,μg |
| R4363 |
T6986 |
T6987 |
punct |
[,Roche |
| R4364 |
T6987 |
T6983 |
parataxis |
Roche,leupeptin |
| R4365 |
T6988 |
T6987 |
punct |
],Roche |
| R4366 |
T6989 |
T6927 |
punct |
),isolated |
| R4367 |
T6990 |
T6927 |
punct |
.,isolated |
| R4368 |
T6992 |
T6993 |
prep |
Following,centrifuged |
| R4369 |
T6994 |
T6992 |
pobj |
homogenization,Following |
| R4370 |
T6995 |
T6994 |
prep |
through,homogenization |
| R4371 |
T6996 |
T6997 |
det |
a,needle |
| R4372 |
T6997 |
T6995 |
pobj |
needle,through |
| R4373 |
T6998 |
T6999 |
nummod |
25,gauge |
| R4374 |
T6999 |
T6997 |
compound |
gauge,needle |
| R4375 |
T7000 |
T6999 |
punct |
-,gauge |
| R4376 |
T7001 |
T7002 |
punct |
(,passes |
| R4377 |
T7002 |
T6994 |
parataxis |
passes,homogenization |
| R4378 |
T7003 |
T7004 |
punct |
~,10 |
| R4379 |
T7004 |
T7002 |
nummod |
10,passes |
| R4380 |
T7005 |
T7002 |
punct |
),passes |
| R4381 |
T7006 |
T6993 |
punct |
", ",centrifuged |
| R4382 |
T7007 |
T6993 |
nsubjpass |
extracts,centrifuged |
| R4383 |
T7008 |
T6993 |
auxpass |
were,centrifuged |
| R4384 |
T7009 |
T6993 |
prep |
at,centrifuged |
| R4385 |
T7010 |
T7011 |
nummod |
1000,g |
| R4386 |
T7011 |
T7009 |
pobj |
g,at |
| R4387 |
T7012 |
T7011 |
punct |
×,g |
| R4388 |
T7013 |
T6993 |
prep |
for,centrifuged |
| R4389 |
T7014 |
T7015 |
nummod |
10,minutes |
| R4390 |
T7015 |
T7013 |
pobj |
minutes,for |
| R4391 |
T7016 |
T6993 |
punct |
", ",centrifuged |
| R4392 |
T7017 |
T6993 |
prep |
at,centrifuged |
| R4393 |
T7018 |
T7019 |
nummod |
4,°C |
| R4394 |
T7019 |
T7017 |
pobj |
°C,at |
| R4395 |
T7020 |
T6993 |
punct |
", ",centrifuged |
| R4396 |
T7021 |
T7022 |
aux |
to,remove |
| R4397 |
T7022 |
T6993 |
advcl |
remove,centrifuged |
| R4398 |
T7023 |
T7022 |
dobj |
debris,remove |
| R4399 |
T7024 |
T6993 |
punct |
.,centrifuged |
| R4400 |
T7026 |
T7027 |
advmod |
Typically,separated |
| R4401 |
T7028 |
T7027 |
punct |
", ",separated |
| R4402 |
T7029 |
T7030 |
quantmod |
20,40 |
| R4403 |
T7030 |
T7032 |
nummod |
40,μg |
| R4404 |
T7031 |
T7030 |
punct |
–,40 |
| R4405 |
T7032 |
T7027 |
nsubjpass |
μg,separated |
| R4406 |
T7033 |
T7032 |
prep |
of,μg |
| R4407 |
T7034 |
T7033 |
pobj |
protein,of |
| R4408 |
T7035 |
T7034 |
punct |
(,protein |
| R4409 |
T7036 |
T7034 |
acl |
determined,protein |
| R4410 |
T7037 |
T7036 |
agent |
by,determined |
| R4411 |
T7038 |
T7039 |
compound |
Bio,rad |
| R4412 |
T7039 |
T7041 |
compound |
rad,Assay |
| R4413 |
T7040 |
T7039 |
punct |
-,rad |
| R4414 |
T7041 |
T7037 |
pobj |
Assay,by |
| R4415 |
T7042 |
T7041 |
compound |
Dc,Assay |
| R4416 |
T7043 |
T7041 |
compound |
Protein,Assay |
| R4417 |
T7044 |
T7027 |
punct |
),separated |
| R4418 |
T7045 |
T7027 |
auxpass |
was,separated |
| R4419 |
T7046 |
T7027 |
prep |
by,separated |
| R4420 |
T7047 |
T7048 |
compound |
SDS,PAGE |
| R4421 |
T7048 |
T7046 |
pobj |
PAGE,by |
| R4422 |
T7049 |
T7048 |
punct |
-,PAGE |
| R4423 |
T7050 |
T7027 |
punct |
", ",separated |
| R4424 |
T7051 |
T7027 |
prep |
for,separated |
| R4425 |
T7052 |
T7053 |
amod |
subsequent,immunoblotting |
| R4426 |
T7053 |
T7051 |
pobj |
immunoblotting,for |
| R4427 |
T7054 |
T7027 |
punct |
", ",separated |
| R4428 |
T7055 |
T7056 |
mark |
as,described |
| R4429 |
T7056 |
T7027 |
advcl |
described,separated |
| R4430 |
T7057 |
T7058 |
punct |
[,12 |
| R4431 |
T7058 |
T7027 |
parataxis |
12,separated |
| R4432 |
T7059 |
T7058 |
punct |
],12 |
| R4433 |
T7060 |
T7027 |
punct |
.,separated |
| R4434 |
T7062 |
T7063 |
nummod |
16.5,% |
| R4435 |
T7063 |
T7064 |
compound |
%,gels |
| R4436 |
T7064 |
T7071 |
nsubjpass |
gels,used |
| R4437 |
T7065 |
T7066 |
compound |
tris,tricine |
| R4438 |
T7066 |
T7064 |
compound |
tricine,gels |
| R4439 |
T7067 |
T7066 |
punct |
-,tricine |
| R4440 |
T7068 |
T7069 |
compound |
SDS,PAGE |
| R4441 |
T7069 |
T7064 |
compound |
PAGE,gels |
| R4442 |
T7070 |
T7069 |
punct |
-,PAGE |
| R4443 |
T7072 |
T7071 |
auxpass |
were,used |
| R4444 |
T7073 |
T7071 |
prep |
for,used |
| R4445 |
T7074 |
T7075 |
compound |
subunit,c |
| R4446 |
T7075 |
T7076 |
compound |
c,experiments |
| R4447 |
T7076 |
T7073 |
pobj |
experiments,for |
| R4448 |
T7077 |
T7076 |
compound |
immunoblotting,experiments |
| R4449 |
T7078 |
T7071 |
punct |
.,used |
