PMC:539297 / 29234-29835 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T6171 0-7 JJ denotes Genomic
T6172 8-11 NN denotes DNA
T6174 12-15 VBD denotes was
T6173 16-25 VBN denotes extracted
T6175 26-30 IN denotes from
T6176 31-35 NN denotes tail
T6177 36-44 NNS denotes biopsies
T6178 45-48 CC denotes and
T6179 49-53 NN denotes cell
T6180 54-61 NNS denotes pellets
T6181 62-64 IN denotes as
T6182 65-74 VBN denotes described
T6183 75-76 -LRB- denotes (
T6184 76-82 NNP denotes Cotman
T6185 83-85 FW denotes et
T6186 86-89 FW denotes al.
T6187 89-91 , denotes ,
T6188 91-95 CD denotes 2002
T6189 95-96 -RRB- denotes )
T6190 96-97 . denotes .
T6191 97-386 sentence denotes Cln3Δex7/8 knock-in allele PCR genotyping was with wild-type primers, WtF (5'-CAGCATCTCCTCAGGGCTA-3') and WtR (5'-CCAACATAGAAAGTAGGGTGTGC-3') to yield a ~250 bp band and knock-in primers, 552F (5'-GAGCTTTGTTCTGGTTGCCTTC-3') and Ex9RA (5'-GCAGTCTCTGCCTCGTTTTCT-3') to yield a ~500 bp band.
T6192 98-106 NN denotes Cln3Δex7
T6194 106-107 HYPH denotes /
T6195 107-108 CD denotes 8
T6196 109-114 VB denotes knock
T6197 114-115 HYPH denotes -
T6198 115-117 RP denotes in
T6193 118-124 NN denotes allele
T6200 125-128 NN denotes PCR
T6199 129-139 NN denotes genotyping
T6201 140-143 VBD denotes was
T6202 144-148 IN denotes with
T6203 149-153 JJ denotes wild
T6205 153-154 HYPH denotes -
T6204 154-158 NN denotes type
T6206 159-166 NNS denotes primers
T6207 166-168 , denotes ,
T6208 168-171 NN denotes WtF
T6209 172-173 -LRB- denotes (
T6210 173-174 CD denotes 5
T6212 174-175 SYM denotes '
T6213 175-176 HYPH denotes -
T6211 176-195 NN denotes CAGCATCTCCTCAGGGCTA
T6214 195-196 HYPH denotes -
T6215 196-197 CD denotes 3
T6216 197-198 SYM denotes '
T6217 198-199 -RRB- denotes )
T6218 200-203 CC denotes and
T6219 204-207 NN denotes WtR
T6220 208-209 -LRB- denotes (
T6221 209-210 CD denotes 5
T6223 210-211 SYM denotes '
T6224 211-212 HYPH denotes -
T6222 212-235 NN denotes CCAACATAGAAAGTAGGGTGTGC
T6225 235-236 HYPH denotes -
T6226 236-237 CD denotes 3
T6227 237-238 SYM denotes '
T6228 238-239 -RRB- denotes )
T6229 240-242 TO denotes to
T6230 243-248 VB denotes yield
T6231 249-250 DT denotes a
T6233 251-252 SYM denotes ~
T6234 252-255 CD denotes 250
T6235 256-258 NN denotes bp
T6232 259-263 NN denotes band
T6236 264-267 CC denotes and
T6237 268-273 VB denotes knock
T6239 273-274 HYPH denotes -
T6240 274-276 RP denotes in
T6238 277-284 NNS denotes primers
T6241 284-286 , denotes ,
T6242 286-290 NN denotes 552F
T6243 291-292 -LRB- denotes (
T6244 292-293 CD denotes 5
T6246 293-294 SYM denotes '
T6247 294-295 HYPH denotes -
T6245 295-317 NN denotes GAGCTTTGTTCTGGTTGCCTTC
T6248 317-318 HYPH denotes -
T6249 318-319 CD denotes 3
T6250 319-320 SYM denotes '
T6251 320-321 -RRB- denotes )
T6252 322-325 CC denotes and
T6253 326-331 NN denotes Ex9RA
T6254 332-333 -LRB- denotes (
T6255 333-334 CD denotes 5
T6257 334-335 SYM denotes '
T6258 335-336 HYPH denotes -
T6256 336-357 NN denotes GCAGTCTCTGCCTCGTTTTCT
T6259 357-358 HYPH denotes -
T6260 358-359 CD denotes 3
T6261 359-360 SYM denotes '
T6262 360-361 -RRB- denotes )
T6263 362-364 TO denotes to
T6264 365-370 VB denotes yield
T6265 371-372 DT denotes a
T6267 373-374 SYM denotes ~
T6268 374-377 CD denotes 500
T6269 378-380 NN denotes bp
T6266 381-385 NN denotes band
T6270 385-386 . denotes .
T6271 386-505 sentence denotes PCR cycling conditions were 95°C for 30 seconds, 58°C for 30 seconds, and 72°C for 35 seconds, repeated for 34 cycles.
T6272 387-390 NN denotes PCR
T6273 391-398 NN denotes cycling
T6274 399-409 NNS denotes conditions
T6275 410-414 VBD denotes were
T6276 415-417 CD denotes 95
T6277 417-419 NN denotes °C
T6278 420-423 IN denotes for
T6279 424-426 CD denotes 30
T6280 427-434 NNS denotes seconds
T6281 434-436 , denotes ,
T6282 436-438 CD denotes 58
T6283 438-440 NN denotes °C
T6284 441-444 IN denotes for
T6285 445-447 CD denotes 30
T6286 448-455 NNS denotes seconds
T6287 455-457 , denotes ,
T6288 457-460 CC denotes and
T6289 461-463 CD denotes 72
T6290 463-465 NN denotes °C
T6291 466-469 IN denotes for
T6292 470-472 CD denotes 35
T6293 473-480 NNS denotes seconds
T6294 480-482 , denotes ,
T6295 482-490 VBN denotes repeated
T6296 491-494 IN denotes for
T6297 495-497 CD denotes 34
T6298 498-504 NNS denotes cycles
T6299 504-505 . denotes .
T6300 505-601 sentence denotes Total RNA isolation and Cln3 RT-PCR primers and procedures have been previously described [12].
T6301 506-511 JJ denotes Total
T6303 512-515 NN denotes RNA
T6302 516-525 NN denotes isolation
T6305 526-529 CC denotes and
T6306 530-534 NN denotes Cln3
T6308 535-537 NN denotes RT
T6310 537-538 HYPH denotes -
T6309 538-541 NN denotes PCR
T6307 542-549 NNS denotes primers
T6311 550-553 CC denotes and
T6312 554-564 NNS denotes procedures
T6313 565-569 VBP denotes have
T6314 570-574 VBN denotes been
T6315 575-585 RB denotes previously
T6304 586-595 VBN denotes described
T6316 596-597 -LRB- denotes [
T6317 597-599 CD denotes 12
T6318 599-600 -RRB- denotes ]
T6319 600-601 . denotes .
R3725 T6171 T6172 amod Genomic,DNA
R3726 T6172 T6173 nsubjpass DNA,extracted
R3727 T6174 T6173 auxpass was,extracted
R3728 T6175 T6173 prep from,extracted
R3729 T6176 T6177 compound tail,biopsies
R3730 T6177 T6175 pobj biopsies,from
R3731 T6178 T6177 cc and,biopsies
R3732 T6179 T6180 compound cell,pellets
R3733 T6180 T6177 conj pellets,biopsies
R3734 T6181 T6182 mark as,described
R3735 T6182 T6173 advcl described,extracted
R3736 T6183 T6184 punct (,Cotman
R3737 T6184 T6173 meta Cotman,extracted
R3738 T6185 T6184 nmod et,Cotman
R3739 T6186 T6184 nmod al.,Cotman
R3740 T6187 T6184 punct ", ",Cotman
R3741 T6188 T6184 nummod 2002,Cotman
R3742 T6189 T6184 punct ),Cotman
R3743 T6190 T6173 punct .,extracted
R3744 T6192 T6193 nmod Cln3Δex7,allele
R3745 T6193 T6199 compound allele,genotyping
R3746 T6194 T6192 punct /,Cln3Δex7
R3747 T6195 T6192 nummod 8,Cln3Δex7
R3748 T6196 T6193 amod knock,allele
R3749 T6197 T6196 punct -,knock
R3750 T6198 T6196 prt in,knock
R3751 T6199 T6201 nsubj genotyping,was
R3752 T6200 T6199 compound PCR,genotyping
R3753 T6202 T6201 prep with,was
R3754 T6203 T6204 amod wild,type
R3755 T6204 T6206 compound type,primers
R3756 T6205 T6204 punct -,type
R3757 T6206 T6202 pobj primers,with
R3758 T6207 T6206 punct ", ",primers
R3759 T6208 T6206 appos WtF,primers
R3760 T6209 T6208 punct (,WtF
R3761 T6210 T6211 nummod 5,CAGCATCTCCTCAGGGCTA
R3762 T6211 T6208 appos CAGCATCTCCTCAGGGCTA,WtF
R3763 T6212 T6210 punct ',5
R3764 T6213 T6211 punct -,CAGCATCTCCTCAGGGCTA
R3765 T6214 T6211 punct -,CAGCATCTCCTCAGGGCTA
R3766 T6215 T6211 nummod 3,CAGCATCTCCTCAGGGCTA
R3767 T6216 T6211 punct ',CAGCATCTCCTCAGGGCTA
R3768 T6217 T6208 punct ),WtF
R3769 T6218 T6208 cc and,WtF
R3770 T6219 T6208 conj WtR,WtF
R3771 T6220 T6219 punct (,WtR
R3772 T6221 T6222 nummod 5,CCAACATAGAAAGTAGGGTGTGC
R3773 T6222 T6219 appos CCAACATAGAAAGTAGGGTGTGC,WtR
R3774 T6223 T6221 punct ',5
R3775 T6224 T6222 punct -,CCAACATAGAAAGTAGGGTGTGC
R3776 T6225 T6222 punct -,CCAACATAGAAAGTAGGGTGTGC
R3777 T6226 T6222 nummod 3,CCAACATAGAAAGTAGGGTGTGC
R3778 T6227 T6222 punct ',CCAACATAGAAAGTAGGGTGTGC
R3779 T6228 T6201 punct ),was
R3780 T6229 T6230 aux to,yield
R3781 T6230 T6201 advcl yield,was
R3782 T6231 T6232 det a,band
R3783 T6232 T6230 dobj band,yield
R3784 T6233 T6234 punct ~,250
R3785 T6234 T6235 nummod 250,bp
R3786 T6235 T6232 compound bp,band
R3787 T6236 T6201 cc and,was
R3788 T6237 T6238 amod knock,primers
R3789 T6238 T6201 conj primers,was
R3790 T6239 T6237 punct -,knock
R3791 T6240 T6237 prt in,knock
R3792 T6241 T6238 punct ", ",primers
R3793 T6242 T6238 appos 552F,primers
R3794 T6243 T6242 punct (,552F
R3795 T6244 T6245 nummod 5,GAGCTTTGTTCTGGTTGCCTTC
R3796 T6245 T6242 appos GAGCTTTGTTCTGGTTGCCTTC,552F
R3797 T6246 T6244 punct ',5
R3798 T6247 T6245 punct -,GAGCTTTGTTCTGGTTGCCTTC
R3799 T6248 T6245 punct -,GAGCTTTGTTCTGGTTGCCTTC
R3800 T6249 T6245 nummod 3,GAGCTTTGTTCTGGTTGCCTTC
R3801 T6250 T6245 punct ',GAGCTTTGTTCTGGTTGCCTTC
R3802 T6251 T6242 punct ),552F
R3803 T6252 T6242 cc and,552F
R3804 T6253 T6242 conj Ex9RA,552F
R3805 T6254 T6253 punct (,Ex9RA
R3806 T6255 T6256 nummod 5,GCAGTCTCTGCCTCGTTTTCT
R3807 T6256 T6253 appos GCAGTCTCTGCCTCGTTTTCT,Ex9RA
R3808 T6257 T6255 punct ',5
R3809 T6258 T6256 punct -,GCAGTCTCTGCCTCGTTTTCT
R3810 T6259 T6256 punct -,GCAGTCTCTGCCTCGTTTTCT
R3811 T6260 T6256 nummod 3,GCAGTCTCTGCCTCGTTTTCT
R3812 T6261 T6256 punct ',GCAGTCTCTGCCTCGTTTTCT
R3813 T6262 T6238 punct ),primers
R3814 T6263 T6264 aux to,yield
R3815 T6264 T6238 advcl yield,primers
R3816 T6265 T6266 det a,band
R3817 T6266 T6264 dobj band,yield
R3818 T6267 T6268 punct ~,500
R3819 T6268 T6269 nummod 500,bp
R3820 T6269 T6266 compound bp,band
R3821 T6270 T6201 punct .,was
R3822 T6272 T6273 compound PCR,cycling
R3823 T6273 T6274 compound cycling,conditions
R3824 T6274 T6275 nsubj conditions,were
R3825 T6276 T6277 nummod 95,°C
R3826 T6277 T6275 attr °C,were
R3827 T6278 T6277 prep for,°C
R3828 T6279 T6280 nummod 30,seconds
R3829 T6280 T6278 pobj seconds,for
R3830 T6281 T6277 punct ", ",°C
R3831 T6282 T6283 nummod 58,°C
R3832 T6283 T6277 conj °C,°C
R3833 T6284 T6283 prep for,°C
R3834 T6285 T6286 nummod 30,seconds
R3835 T6286 T6284 pobj seconds,for
R3836 T6287 T6283 punct ", ",°C
R3837 T6288 T6283 cc and,°C
R3838 T6289 T6290 nummod 72,°C
R3839 T6290 T6283 conj °C,°C
R3840 T6291 T6290 prep for,°C
R3841 T6292 T6293 nummod 35,seconds
R3842 T6293 T6291 pobj seconds,for
R3843 T6294 T6277 punct ", ",°C
R3844 T6295 T6277 acl repeated,°C
R3845 T6296 T6295 prep for,repeated
R3846 T6297 T6298 nummod 34,cycles
R3847 T6298 T6296 pobj cycles,for
R3848 T6299 T6275 punct .,were
R3849 T6301 T6302 amod Total,isolation
R3850 T6302 T6304 nsubjpass isolation,described
R3851 T6303 T6302 compound RNA,isolation
R3852 T6305 T6302 cc and,isolation
R3853 T6306 T6307 compound Cln3,primers
R3854 T6307 T6302 conj primers,isolation
R3855 T6308 T6309 compound RT,PCR
R3856 T6309 T6307 compound PCR,primers
R3857 T6310 T6309 punct -,PCR
R3858 T6311 T6307 cc and,primers
R3859 T6312 T6307 conj procedures,primers
R3860 T6313 T6304 aux have,described
R3861 T6314 T6304 auxpass been,described
R3862 T6315 T6304 advmod previously,described
R3863 T6316 T6317 punct [,12
R3864 T6317 T6304 parataxis 12,described
R3865 T6318 T6317 punct ],12
R3866 T6319 T6304 punct .,described