Id |
Subject |
Object |
Predicate |
Lexical cue |
T6171 |
0-7 |
JJ |
denotes |
Genomic |
T6172 |
8-11 |
NN |
denotes |
DNA |
T6174 |
12-15 |
VBD |
denotes |
was |
T6173 |
16-25 |
VBN |
denotes |
extracted |
T6175 |
26-30 |
IN |
denotes |
from |
T6176 |
31-35 |
NN |
denotes |
tail |
T6177 |
36-44 |
NNS |
denotes |
biopsies |
T6178 |
45-48 |
CC |
denotes |
and |
T6179 |
49-53 |
NN |
denotes |
cell |
T6180 |
54-61 |
NNS |
denotes |
pellets |
T6181 |
62-64 |
IN |
denotes |
as |
T6182 |
65-74 |
VBN |
denotes |
described |
T6183 |
75-76 |
-LRB- |
denotes |
( |
T6184 |
76-82 |
NNP |
denotes |
Cotman |
T6185 |
83-85 |
FW |
denotes |
et |
T6186 |
86-89 |
FW |
denotes |
al. |
T6187 |
89-91 |
, |
denotes |
, |
T6188 |
91-95 |
CD |
denotes |
2002 |
T6189 |
95-96 |
-RRB- |
denotes |
) |
T6190 |
96-97 |
. |
denotes |
. |
T6191 |
97-386 |
sentence |
denotes |
Cln3Δex7/8 knock-in allele PCR genotyping was with wild-type primers, WtF (5'-CAGCATCTCCTCAGGGCTA-3') and WtR (5'-CCAACATAGAAAGTAGGGTGTGC-3') to yield a ~250 bp band and knock-in primers, 552F (5'-GAGCTTTGTTCTGGTTGCCTTC-3') and Ex9RA (5'-GCAGTCTCTGCCTCGTTTTCT-3') to yield a ~500 bp band. |
T6192 |
98-106 |
NN |
denotes |
Cln3Δex7 |
T6194 |
106-107 |
HYPH |
denotes |
/ |
T6195 |
107-108 |
CD |
denotes |
8 |
T6196 |
109-114 |
VB |
denotes |
knock |
T6197 |
114-115 |
HYPH |
denotes |
- |
T6198 |
115-117 |
RP |
denotes |
in |
T6193 |
118-124 |
NN |
denotes |
allele |
T6200 |
125-128 |
NN |
denotes |
PCR |
T6199 |
129-139 |
NN |
denotes |
genotyping |
T6201 |
140-143 |
VBD |
denotes |
was |
T6202 |
144-148 |
IN |
denotes |
with |
T6203 |
149-153 |
JJ |
denotes |
wild |
T6205 |
153-154 |
HYPH |
denotes |
- |
T6204 |
154-158 |
NN |
denotes |
type |
T6206 |
159-166 |
NNS |
denotes |
primers |
T6207 |
166-168 |
, |
denotes |
, |
T6208 |
168-171 |
NN |
denotes |
WtF |
T6209 |
172-173 |
-LRB- |
denotes |
( |
T6210 |
173-174 |
CD |
denotes |
5 |
T6212 |
174-175 |
SYM |
denotes |
' |
T6213 |
175-176 |
HYPH |
denotes |
- |
T6211 |
176-195 |
NN |
denotes |
CAGCATCTCCTCAGGGCTA |
T6214 |
195-196 |
HYPH |
denotes |
- |
T6215 |
196-197 |
CD |
denotes |
3 |
T6216 |
197-198 |
SYM |
denotes |
' |
T6217 |
198-199 |
-RRB- |
denotes |
) |
T6218 |
200-203 |
CC |
denotes |
and |
T6219 |
204-207 |
NN |
denotes |
WtR |
T6220 |
208-209 |
-LRB- |
denotes |
( |
T6221 |
209-210 |
CD |
denotes |
5 |
T6223 |
210-211 |
SYM |
denotes |
' |
T6224 |
211-212 |
HYPH |
denotes |
- |
T6222 |
212-235 |
NN |
denotes |
CCAACATAGAAAGTAGGGTGTGC |
T6225 |
235-236 |
HYPH |
denotes |
- |
T6226 |
236-237 |
CD |
denotes |
3 |
T6227 |
237-238 |
SYM |
denotes |
' |
T6228 |
238-239 |
-RRB- |
denotes |
) |
T6229 |
240-242 |
TO |
denotes |
to |
T6230 |
243-248 |
VB |
denotes |
yield |
T6231 |
249-250 |
DT |
denotes |
a |
T6233 |
251-252 |
SYM |
denotes |
~ |
T6234 |
252-255 |
CD |
denotes |
250 |
T6235 |
256-258 |
NN |
denotes |
bp |
T6232 |
259-263 |
NN |
denotes |
band |
T6236 |
264-267 |
CC |
denotes |
and |
T6237 |
268-273 |
VB |
denotes |
knock |
T6239 |
273-274 |
HYPH |
denotes |
- |
T6240 |
274-276 |
RP |
denotes |
in |
T6238 |
277-284 |
NNS |
denotes |
primers |
T6241 |
284-286 |
, |
denotes |
, |
T6242 |
286-290 |
NN |
denotes |
552F |
T6243 |
291-292 |
-LRB- |
denotes |
( |
T6244 |
292-293 |
CD |
denotes |
5 |
T6246 |
293-294 |
SYM |
denotes |
' |
T6247 |
294-295 |
HYPH |
denotes |
- |
T6245 |
295-317 |
NN |
denotes |
GAGCTTTGTTCTGGTTGCCTTC |
T6248 |
317-318 |
HYPH |
denotes |
- |
T6249 |
318-319 |
CD |
denotes |
3 |
T6250 |
319-320 |
SYM |
denotes |
' |
T6251 |
320-321 |
-RRB- |
denotes |
) |
T6252 |
322-325 |
CC |
denotes |
and |
T6253 |
326-331 |
NN |
denotes |
Ex9RA |
T6254 |
332-333 |
-LRB- |
denotes |
( |
T6255 |
333-334 |
CD |
denotes |
5 |
T6257 |
334-335 |
SYM |
denotes |
' |
T6258 |
335-336 |
HYPH |
denotes |
- |
T6256 |
336-357 |
NN |
denotes |
GCAGTCTCTGCCTCGTTTTCT |
T6259 |
357-358 |
HYPH |
denotes |
- |
T6260 |
358-359 |
CD |
denotes |
3 |
T6261 |
359-360 |
SYM |
denotes |
' |
T6262 |
360-361 |
-RRB- |
denotes |
) |
T6263 |
362-364 |
TO |
denotes |
to |
T6264 |
365-370 |
VB |
denotes |
yield |
T6265 |
371-372 |
DT |
denotes |
a |
T6267 |
373-374 |
SYM |
denotes |
~ |
T6268 |
374-377 |
CD |
denotes |
500 |
T6269 |
378-380 |
NN |
denotes |
bp |
T6266 |
381-385 |
NN |
denotes |
band |
T6270 |
385-386 |
. |
denotes |
. |
T6271 |
386-505 |
sentence |
denotes |
PCR cycling conditions were 95°C for 30 seconds, 58°C for 30 seconds, and 72°C for 35 seconds, repeated for 34 cycles. |
T6272 |
387-390 |
NN |
denotes |
PCR |
T6273 |
391-398 |
NN |
denotes |
cycling |
T6274 |
399-409 |
NNS |
denotes |
conditions |
T6275 |
410-414 |
VBD |
denotes |
were |
T6276 |
415-417 |
CD |
denotes |
95 |
T6277 |
417-419 |
NN |
denotes |
°C |
T6278 |
420-423 |
IN |
denotes |
for |
T6279 |
424-426 |
CD |
denotes |
30 |
T6280 |
427-434 |
NNS |
denotes |
seconds |
T6281 |
434-436 |
, |
denotes |
, |
T6282 |
436-438 |
CD |
denotes |
58 |
T6283 |
438-440 |
NN |
denotes |
°C |
T6284 |
441-444 |
IN |
denotes |
for |
T6285 |
445-447 |
CD |
denotes |
30 |
T6286 |
448-455 |
NNS |
denotes |
seconds |
T6287 |
455-457 |
, |
denotes |
, |
T6288 |
457-460 |
CC |
denotes |
and |
T6289 |
461-463 |
CD |
denotes |
72 |
T6290 |
463-465 |
NN |
denotes |
°C |
T6291 |
466-469 |
IN |
denotes |
for |
T6292 |
470-472 |
CD |
denotes |
35 |
T6293 |
473-480 |
NNS |
denotes |
seconds |
T6294 |
480-482 |
, |
denotes |
, |
T6295 |
482-490 |
VBN |
denotes |
repeated |
T6296 |
491-494 |
IN |
denotes |
for |
T6297 |
495-497 |
CD |
denotes |
34 |
T6298 |
498-504 |
NNS |
denotes |
cycles |
T6299 |
504-505 |
. |
denotes |
. |
T6300 |
505-601 |
sentence |
denotes |
Total RNA isolation and Cln3 RT-PCR primers and procedures have been previously described [12]. |
T6301 |
506-511 |
JJ |
denotes |
Total |
T6303 |
512-515 |
NN |
denotes |
RNA |
T6302 |
516-525 |
NN |
denotes |
isolation |
T6305 |
526-529 |
CC |
denotes |
and |
T6306 |
530-534 |
NN |
denotes |
Cln3 |
T6308 |
535-537 |
NN |
denotes |
RT |
T6310 |
537-538 |
HYPH |
denotes |
- |
T6309 |
538-541 |
NN |
denotes |
PCR |
T6307 |
542-549 |
NNS |
denotes |
primers |
T6311 |
550-553 |
CC |
denotes |
and |
T6312 |
554-564 |
NNS |
denotes |
procedures |
T6313 |
565-569 |
VBP |
denotes |
have |
T6314 |
570-574 |
VBN |
denotes |
been |
T6315 |
575-585 |
RB |
denotes |
previously |
T6304 |
586-595 |
VBN |
denotes |
described |
T6316 |
596-597 |
-LRB- |
denotes |
[ |
T6317 |
597-599 |
CD |
denotes |
12 |
T6318 |
599-600 |
-RRB- |
denotes |
] |
T6319 |
600-601 |
. |
denotes |
. |
R3725 |
T6171 |
T6172 |
amod |
Genomic,DNA |
R3726 |
T6172 |
T6173 |
nsubjpass |
DNA,extracted |
R3727 |
T6174 |
T6173 |
auxpass |
was,extracted |
R3728 |
T6175 |
T6173 |
prep |
from,extracted |
R3729 |
T6176 |
T6177 |
compound |
tail,biopsies |
R3730 |
T6177 |
T6175 |
pobj |
biopsies,from |
R3731 |
T6178 |
T6177 |
cc |
and,biopsies |
R3732 |
T6179 |
T6180 |
compound |
cell,pellets |
R3733 |
T6180 |
T6177 |
conj |
pellets,biopsies |
R3734 |
T6181 |
T6182 |
mark |
as,described |
R3735 |
T6182 |
T6173 |
advcl |
described,extracted |
R3736 |
T6183 |
T6184 |
punct |
(,Cotman |
R3737 |
T6184 |
T6173 |
meta |
Cotman,extracted |
R3738 |
T6185 |
T6184 |
nmod |
et,Cotman |
R3739 |
T6186 |
T6184 |
nmod |
al.,Cotman |
R3740 |
T6187 |
T6184 |
punct |
", ",Cotman |
R3741 |
T6188 |
T6184 |
nummod |
2002,Cotman |
R3742 |
T6189 |
T6184 |
punct |
),Cotman |
R3743 |
T6190 |
T6173 |
punct |
.,extracted |
R3744 |
T6192 |
T6193 |
nmod |
Cln3Δex7,allele |
R3745 |
T6193 |
T6199 |
compound |
allele,genotyping |
R3746 |
T6194 |
T6192 |
punct |
/,Cln3Δex7 |
R3747 |
T6195 |
T6192 |
nummod |
8,Cln3Δex7 |
R3748 |
T6196 |
T6193 |
amod |
knock,allele |
R3749 |
T6197 |
T6196 |
punct |
-,knock |
R3750 |
T6198 |
T6196 |
prt |
in,knock |
R3751 |
T6199 |
T6201 |
nsubj |
genotyping,was |
R3752 |
T6200 |
T6199 |
compound |
PCR,genotyping |
R3753 |
T6202 |
T6201 |
prep |
with,was |
R3754 |
T6203 |
T6204 |
amod |
wild,type |
R3755 |
T6204 |
T6206 |
compound |
type,primers |
R3756 |
T6205 |
T6204 |
punct |
-,type |
R3757 |
T6206 |
T6202 |
pobj |
primers,with |
R3758 |
T6207 |
T6206 |
punct |
", ",primers |
R3759 |
T6208 |
T6206 |
appos |
WtF,primers |
R3760 |
T6209 |
T6208 |
punct |
(,WtF |
R3761 |
T6210 |
T6211 |
nummod |
5,CAGCATCTCCTCAGGGCTA |
R3762 |
T6211 |
T6208 |
appos |
CAGCATCTCCTCAGGGCTA,WtF |
R3763 |
T6212 |
T6210 |
punct |
',5 |
R3764 |
T6213 |
T6211 |
punct |
-,CAGCATCTCCTCAGGGCTA |
R3765 |
T6214 |
T6211 |
punct |
-,CAGCATCTCCTCAGGGCTA |
R3766 |
T6215 |
T6211 |
nummod |
3,CAGCATCTCCTCAGGGCTA |
R3767 |
T6216 |
T6211 |
punct |
',CAGCATCTCCTCAGGGCTA |
R3768 |
T6217 |
T6208 |
punct |
),WtF |
R3769 |
T6218 |
T6208 |
cc |
and,WtF |
R3770 |
T6219 |
T6208 |
conj |
WtR,WtF |
R3771 |
T6220 |
T6219 |
punct |
(,WtR |
R3772 |
T6221 |
T6222 |
nummod |
5,CCAACATAGAAAGTAGGGTGTGC |
R3773 |
T6222 |
T6219 |
appos |
CCAACATAGAAAGTAGGGTGTGC,WtR |
R3774 |
T6223 |
T6221 |
punct |
',5 |
R3775 |
T6224 |
T6222 |
punct |
-,CCAACATAGAAAGTAGGGTGTGC |
R3776 |
T6225 |
T6222 |
punct |
-,CCAACATAGAAAGTAGGGTGTGC |
R3777 |
T6226 |
T6222 |
nummod |
3,CCAACATAGAAAGTAGGGTGTGC |
R3778 |
T6227 |
T6222 |
punct |
',CCAACATAGAAAGTAGGGTGTGC |
R3779 |
T6228 |
T6201 |
punct |
),was |
R3780 |
T6229 |
T6230 |
aux |
to,yield |
R3781 |
T6230 |
T6201 |
advcl |
yield,was |
R3782 |
T6231 |
T6232 |
det |
a,band |
R3783 |
T6232 |
T6230 |
dobj |
band,yield |
R3784 |
T6233 |
T6234 |
punct |
~,250 |
R3785 |
T6234 |
T6235 |
nummod |
250,bp |
R3786 |
T6235 |
T6232 |
compound |
bp,band |
R3787 |
T6236 |
T6201 |
cc |
and,was |
R3788 |
T6237 |
T6238 |
amod |
knock,primers |
R3789 |
T6238 |
T6201 |
conj |
primers,was |
R3790 |
T6239 |
T6237 |
punct |
-,knock |
R3791 |
T6240 |
T6237 |
prt |
in,knock |
R3792 |
T6241 |
T6238 |
punct |
", ",primers |
R3793 |
T6242 |
T6238 |
appos |
552F,primers |
R3794 |
T6243 |
T6242 |
punct |
(,552F |
R3795 |
T6244 |
T6245 |
nummod |
5,GAGCTTTGTTCTGGTTGCCTTC |
R3796 |
T6245 |
T6242 |
appos |
GAGCTTTGTTCTGGTTGCCTTC,552F |
R3797 |
T6246 |
T6244 |
punct |
',5 |
R3798 |
T6247 |
T6245 |
punct |
-,GAGCTTTGTTCTGGTTGCCTTC |
R3799 |
T6248 |
T6245 |
punct |
-,GAGCTTTGTTCTGGTTGCCTTC |
R3800 |
T6249 |
T6245 |
nummod |
3,GAGCTTTGTTCTGGTTGCCTTC |
R3801 |
T6250 |
T6245 |
punct |
',GAGCTTTGTTCTGGTTGCCTTC |
R3802 |
T6251 |
T6242 |
punct |
),552F |
R3803 |
T6252 |
T6242 |
cc |
and,552F |
R3804 |
T6253 |
T6242 |
conj |
Ex9RA,552F |
R3805 |
T6254 |
T6253 |
punct |
(,Ex9RA |
R3806 |
T6255 |
T6256 |
nummod |
5,GCAGTCTCTGCCTCGTTTTCT |
R3807 |
T6256 |
T6253 |
appos |
GCAGTCTCTGCCTCGTTTTCT,Ex9RA |
R3808 |
T6257 |
T6255 |
punct |
',5 |
R3809 |
T6258 |
T6256 |
punct |
-,GCAGTCTCTGCCTCGTTTTCT |
R3810 |
T6259 |
T6256 |
punct |
-,GCAGTCTCTGCCTCGTTTTCT |
R3811 |
T6260 |
T6256 |
nummod |
3,GCAGTCTCTGCCTCGTTTTCT |
R3812 |
T6261 |
T6256 |
punct |
',GCAGTCTCTGCCTCGTTTTCT |
R3813 |
T6262 |
T6238 |
punct |
),primers |
R3814 |
T6263 |
T6264 |
aux |
to,yield |
R3815 |
T6264 |
T6238 |
advcl |
yield,primers |
R3816 |
T6265 |
T6266 |
det |
a,band |
R3817 |
T6266 |
T6264 |
dobj |
band,yield |
R3818 |
T6267 |
T6268 |
punct |
~,500 |
R3819 |
T6268 |
T6269 |
nummod |
500,bp |
R3820 |
T6269 |
T6266 |
compound |
bp,band |
R3821 |
T6270 |
T6201 |
punct |
.,was |
R3822 |
T6272 |
T6273 |
compound |
PCR,cycling |
R3823 |
T6273 |
T6274 |
compound |
cycling,conditions |
R3824 |
T6274 |
T6275 |
nsubj |
conditions,were |
R3825 |
T6276 |
T6277 |
nummod |
95,°C |
R3826 |
T6277 |
T6275 |
attr |
°C,were |
R3827 |
T6278 |
T6277 |
prep |
for,°C |
R3828 |
T6279 |
T6280 |
nummod |
30,seconds |
R3829 |
T6280 |
T6278 |
pobj |
seconds,for |
R3830 |
T6281 |
T6277 |
punct |
", ",°C |
R3831 |
T6282 |
T6283 |
nummod |
58,°C |
R3832 |
T6283 |
T6277 |
conj |
°C,°C |
R3833 |
T6284 |
T6283 |
prep |
for,°C |
R3834 |
T6285 |
T6286 |
nummod |
30,seconds |
R3835 |
T6286 |
T6284 |
pobj |
seconds,for |
R3836 |
T6287 |
T6283 |
punct |
", ",°C |
R3837 |
T6288 |
T6283 |
cc |
and,°C |
R3838 |
T6289 |
T6290 |
nummod |
72,°C |
R3839 |
T6290 |
T6283 |
conj |
°C,°C |
R3840 |
T6291 |
T6290 |
prep |
for,°C |
R3841 |
T6292 |
T6293 |
nummod |
35,seconds |
R3842 |
T6293 |
T6291 |
pobj |
seconds,for |
R3843 |
T6294 |
T6277 |
punct |
", ",°C |
R3844 |
T6295 |
T6277 |
acl |
repeated,°C |
R3845 |
T6296 |
T6295 |
prep |
for,repeated |
R3846 |
T6297 |
T6298 |
nummod |
34,cycles |
R3847 |
T6298 |
T6296 |
pobj |
cycles,for |
R3848 |
T6299 |
T6275 |
punct |
.,were |
R3849 |
T6301 |
T6302 |
amod |
Total,isolation |
R3850 |
T6302 |
T6304 |
nsubjpass |
isolation,described |
R3851 |
T6303 |
T6302 |
compound |
RNA,isolation |
R3852 |
T6305 |
T6302 |
cc |
and,isolation |
R3853 |
T6306 |
T6307 |
compound |
Cln3,primers |
R3854 |
T6307 |
T6302 |
conj |
primers,isolation |
R3855 |
T6308 |
T6309 |
compound |
RT,PCR |
R3856 |
T6309 |
T6307 |
compound |
PCR,primers |
R3857 |
T6310 |
T6309 |
punct |
-,PCR |
R3858 |
T6311 |
T6307 |
cc |
and,primers |
R3859 |
T6312 |
T6307 |
conj |
procedures,primers |
R3860 |
T6313 |
T6304 |
aux |
have,described |
R3861 |
T6314 |
T6304 |
auxpass |
been,described |
R3862 |
T6315 |
T6304 |
advmod |
previously,described |
R3863 |
T6316 |
T6317 |
punct |
[,12 |
R3864 |
T6317 |
T6304 |
parataxis |
12,described |
R3865 |
T6318 |
T6317 |
punct |
],12 |
R3866 |
T6319 |
T6304 |
punct |
.,described |