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PMC:539297 / 29212-29835 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T6165 0-10 NN denotes Genotyping
T6166 11-14 CC denotes and
T6167 15-17 NN denotes RT
T6169 17-18 HYPH denotes -
T6168 18-21 NN denotes PCR
T6170 21-119 sentence denotes Genomic DNA was extracted from tail biopsies and cell pellets as described (Cotman et al., 2002).
T6171 22-29 JJ denotes Genomic
T6172 30-33 NN denotes DNA
T6174 34-37 VBD denotes was
T6173 38-47 VBN denotes extracted
T6175 48-52 IN denotes from
T6176 53-57 NN denotes tail
T6177 58-66 NNS denotes biopsies
T6178 67-70 CC denotes and
T6179 71-75 NN denotes cell
T6180 76-83 NNS denotes pellets
T6181 84-86 IN denotes as
T6182 87-96 VBN denotes described
T6183 97-98 -LRB- denotes (
T6184 98-104 NNP denotes Cotman
T6185 105-107 FW denotes et
T6186 108-111 FW denotes al.
T6187 111-113 , denotes ,
T6188 113-117 CD denotes 2002
T6189 117-118 -RRB- denotes )
T6190 118-119 . denotes .
T6191 119-408 sentence denotes Cln3Δex7/8 knock-in allele PCR genotyping was with wild-type primers, WtF (5'-CAGCATCTCCTCAGGGCTA-3') and WtR (5'-CCAACATAGAAAGTAGGGTGTGC-3') to yield a ~250 bp band and knock-in primers, 552F (5'-GAGCTTTGTTCTGGTTGCCTTC-3') and Ex9RA (5'-GCAGTCTCTGCCTCGTTTTCT-3') to yield a ~500 bp band.
T6192 120-128 NN denotes Cln3Δex7
T6194 128-129 HYPH denotes /
T6195 129-130 CD denotes 8
T6196 131-136 VB denotes knock
T6197 136-137 HYPH denotes -
T6198 137-139 RP denotes in
T6193 140-146 NN denotes allele
T6200 147-150 NN denotes PCR
T6199 151-161 NN denotes genotyping
T6201 162-165 VBD denotes was
T6202 166-170 IN denotes with
T6203 171-175 JJ denotes wild
T6205 175-176 HYPH denotes -
T6204 176-180 NN denotes type
T6206 181-188 NNS denotes primers
T6207 188-190 , denotes ,
T6208 190-193 NN denotes WtF
T6209 194-195 -LRB- denotes (
T6210 195-196 CD denotes 5
T6212 196-197 SYM denotes '
T6213 197-198 HYPH denotes -
T6211 198-217 NN denotes CAGCATCTCCTCAGGGCTA
T6214 217-218 HYPH denotes -
T6215 218-219 CD denotes 3
T6216 219-220 SYM denotes '
T6217 220-221 -RRB- denotes )
T6218 222-225 CC denotes and
T6219 226-229 NN denotes WtR
T6220 230-231 -LRB- denotes (
T6221 231-232 CD denotes 5
T6223 232-233 SYM denotes '
T6224 233-234 HYPH denotes -
T6222 234-257 NN denotes CCAACATAGAAAGTAGGGTGTGC
T6225 257-258 HYPH denotes -
T6226 258-259 CD denotes 3
T6227 259-260 SYM denotes '
T6228 260-261 -RRB- denotes )
T6229 262-264 TO denotes to
T6230 265-270 VB denotes yield
T6231 271-272 DT denotes a
T6233 273-274 SYM denotes ~
T6234 274-277 CD denotes 250
T6235 278-280 NN denotes bp
T6232 281-285 NN denotes band
T6236 286-289 CC denotes and
T6237 290-295 VB denotes knock
T6239 295-296 HYPH denotes -
T6240 296-298 RP denotes in
T6238 299-306 NNS denotes primers
T6241 306-308 , denotes ,
T6242 308-312 NN denotes 552F
T6243 313-314 -LRB- denotes (
T6244 314-315 CD denotes 5
T6246 315-316 SYM denotes '
T6247 316-317 HYPH denotes -
T6245 317-339 NN denotes GAGCTTTGTTCTGGTTGCCTTC
T6248 339-340 HYPH denotes -
T6249 340-341 CD denotes 3
T6250 341-342 SYM denotes '
T6251 342-343 -RRB- denotes )
T6252 344-347 CC denotes and
T6253 348-353 NN denotes Ex9RA
T6254 354-355 -LRB- denotes (
T6255 355-356 CD denotes 5
T6257 356-357 SYM denotes '
T6258 357-358 HYPH denotes -
T6256 358-379 NN denotes GCAGTCTCTGCCTCGTTTTCT
T6259 379-380 HYPH denotes -
T6260 380-381 CD denotes 3
T6261 381-382 SYM denotes '
T6262 382-383 -RRB- denotes )
T6263 384-386 TO denotes to
T6264 387-392 VB denotes yield
T6265 393-394 DT denotes a
T6267 395-396 SYM denotes ~
T6268 396-399 CD denotes 500
T6269 400-402 NN denotes bp
T6266 403-407 NN denotes band
T6270 407-408 . denotes .
T6271 408-527 sentence denotes PCR cycling conditions were 95°C for 30 seconds, 58°C for 30 seconds, and 72°C for 35 seconds, repeated for 34 cycles.
T6272 409-412 NN denotes PCR
T6273 413-420 NN denotes cycling
T6274 421-431 NNS denotes conditions
T6275 432-436 VBD denotes were
T6276 437-439 CD denotes 95
T6277 439-441 NN denotes °C
T6278 442-445 IN denotes for
T6279 446-448 CD denotes 30
T6280 449-456 NNS denotes seconds
T6281 456-458 , denotes ,
T6282 458-460 CD denotes 58
T6283 460-462 NN denotes °C
T6284 463-466 IN denotes for
T6285 467-469 CD denotes 30
T6286 470-477 NNS denotes seconds
T6287 477-479 , denotes ,
T6288 479-482 CC denotes and
T6289 483-485 CD denotes 72
T6290 485-487 NN denotes °C
T6291 488-491 IN denotes for
T6292 492-494 CD denotes 35
T6293 495-502 NNS denotes seconds
T6294 502-504 , denotes ,
T6295 504-512 VBN denotes repeated
T6296 513-516 IN denotes for
T6297 517-519 CD denotes 34
T6298 520-526 NNS denotes cycles
T6299 526-527 . denotes .
T6300 527-623 sentence denotes Total RNA isolation and Cln3 RT-PCR primers and procedures have been previously described [12].
T6301 528-533 JJ denotes Total
T6303 534-537 NN denotes RNA
T6302 538-547 NN denotes isolation
T6305 548-551 CC denotes and
T6306 552-556 NN denotes Cln3
T6308 557-559 NN denotes RT
T6310 559-560 HYPH denotes -
T6309 560-563 NN denotes PCR
T6307 564-571 NNS denotes primers
T6311 572-575 CC denotes and
T6312 576-586 NNS denotes procedures
T6313 587-591 VBP denotes have
T6314 592-596 VBN denotes been
T6315 597-607 RB denotes previously
T6304 608-617 VBN denotes described
T6316 618-619 -LRB- denotes [
T6317 619-621 CD denotes 12
T6318 621-622 -RRB- denotes ]
T6319 622-623 . denotes .
R3721 T6166 T6165 cc and,Genotyping
R3722 T6167 T6168 compound RT,PCR
R3723 T6168 T6165 conj PCR,Genotyping
R3724 T6169 T6168 punct -,PCR
R3725 T6171 T6172 amod Genomic,DNA
R3726 T6172 T6173 nsubjpass DNA,extracted
R3727 T6174 T6173 auxpass was,extracted
R3728 T6175 T6173 prep from,extracted
R3729 T6176 T6177 compound tail,biopsies
R3730 T6177 T6175 pobj biopsies,from
R3731 T6178 T6177 cc and,biopsies
R3732 T6179 T6180 compound cell,pellets
R3733 T6180 T6177 conj pellets,biopsies
R3734 T6181 T6182 mark as,described
R3735 T6182 T6173 advcl described,extracted
R3736 T6183 T6184 punct (,Cotman
R3737 T6184 T6173 meta Cotman,extracted
R3738 T6185 T6184 nmod et,Cotman
R3739 T6186 T6184 nmod al.,Cotman
R3740 T6187 T6184 punct ", ",Cotman
R3741 T6188 T6184 nummod 2002,Cotman
R3742 T6189 T6184 punct ),Cotman
R3743 T6190 T6173 punct .,extracted
R3744 T6192 T6193 nmod Cln3Δex7,allele
R3745 T6193 T6199 compound allele,genotyping
R3746 T6194 T6192 punct /,Cln3Δex7
R3747 T6195 T6192 nummod 8,Cln3Δex7
R3748 T6196 T6193 amod knock,allele
R3749 T6197 T6196 punct -,knock
R3750 T6198 T6196 prt in,knock
R3751 T6199 T6201 nsubj genotyping,was
R3752 T6200 T6199 compound PCR,genotyping
R3753 T6202 T6201 prep with,was
R3754 T6203 T6204 amod wild,type
R3755 T6204 T6206 compound type,primers
R3756 T6205 T6204 punct -,type
R3757 T6206 T6202 pobj primers,with
R3758 T6207 T6206 punct ", ",primers
R3759 T6208 T6206 appos WtF,primers
R3760 T6209 T6208 punct (,WtF
R3761 T6210 T6211 nummod 5,CAGCATCTCCTCAGGGCTA
R3762 T6211 T6208 appos CAGCATCTCCTCAGGGCTA,WtF
R3763 T6212 T6210 punct ',5
R3764 T6213 T6211 punct -,CAGCATCTCCTCAGGGCTA
R3765 T6214 T6211 punct -,CAGCATCTCCTCAGGGCTA
R3766 T6215 T6211 nummod 3,CAGCATCTCCTCAGGGCTA
R3767 T6216 T6211 punct ',CAGCATCTCCTCAGGGCTA
R3768 T6217 T6208 punct ),WtF
R3769 T6218 T6208 cc and,WtF
R3770 T6219 T6208 conj WtR,WtF
R3771 T6220 T6219 punct (,WtR
R3772 T6221 T6222 nummod 5,CCAACATAGAAAGTAGGGTGTGC
R3773 T6222 T6219 appos CCAACATAGAAAGTAGGGTGTGC,WtR
R3774 T6223 T6221 punct ',5
R3775 T6224 T6222 punct -,CCAACATAGAAAGTAGGGTGTGC
R3776 T6225 T6222 punct -,CCAACATAGAAAGTAGGGTGTGC
R3777 T6226 T6222 nummod 3,CCAACATAGAAAGTAGGGTGTGC
R3778 T6227 T6222 punct ',CCAACATAGAAAGTAGGGTGTGC
R3779 T6228 T6201 punct ),was
R3780 T6229 T6230 aux to,yield
R3781 T6230 T6201 advcl yield,was
R3782 T6231 T6232 det a,band
R3783 T6232 T6230 dobj band,yield
R3784 T6233 T6234 punct ~,250
R3785 T6234 T6235 nummod 250,bp
R3786 T6235 T6232 compound bp,band
R3787 T6236 T6201 cc and,was
R3788 T6237 T6238 amod knock,primers
R3789 T6238 T6201 conj primers,was
R3790 T6239 T6237 punct -,knock
R3791 T6240 T6237 prt in,knock
R3792 T6241 T6238 punct ", ",primers
R3793 T6242 T6238 appos 552F,primers
R3794 T6243 T6242 punct (,552F
R3795 T6244 T6245 nummod 5,GAGCTTTGTTCTGGTTGCCTTC
R3796 T6245 T6242 appos GAGCTTTGTTCTGGTTGCCTTC,552F
R3797 T6246 T6244 punct ',5
R3798 T6247 T6245 punct -,GAGCTTTGTTCTGGTTGCCTTC
R3799 T6248 T6245 punct -,GAGCTTTGTTCTGGTTGCCTTC
R3800 T6249 T6245 nummod 3,GAGCTTTGTTCTGGTTGCCTTC
R3801 T6250 T6245 punct ',GAGCTTTGTTCTGGTTGCCTTC
R3802 T6251 T6242 punct ),552F
R3803 T6252 T6242 cc and,552F
R3804 T6253 T6242 conj Ex9RA,552F
R3805 T6254 T6253 punct (,Ex9RA
R3806 T6255 T6256 nummod 5,GCAGTCTCTGCCTCGTTTTCT
R3807 T6256 T6253 appos GCAGTCTCTGCCTCGTTTTCT,Ex9RA
R3808 T6257 T6255 punct ',5
R3809 T6258 T6256 punct -,GCAGTCTCTGCCTCGTTTTCT
R3810 T6259 T6256 punct -,GCAGTCTCTGCCTCGTTTTCT
R3811 T6260 T6256 nummod 3,GCAGTCTCTGCCTCGTTTTCT
R3812 T6261 T6256 punct ',GCAGTCTCTGCCTCGTTTTCT
R3813 T6262 T6238 punct ),primers
R3814 T6263 T6264 aux to,yield
R3815 T6264 T6238 advcl yield,primers
R3816 T6265 T6266 det a,band
R3817 T6266 T6264 dobj band,yield
R3818 T6267 T6268 punct ~,500
R3819 T6268 T6269 nummod 500,bp
R3820 T6269 T6266 compound bp,band
R3821 T6270 T6201 punct .,was
R3822 T6272 T6273 compound PCR,cycling
R3823 T6273 T6274 compound cycling,conditions
R3824 T6274 T6275 nsubj conditions,were
R3825 T6276 T6277 nummod 95,°C
R3826 T6277 T6275 attr °C,were
R3827 T6278 T6277 prep for,°C
R3828 T6279 T6280 nummod 30,seconds
R3829 T6280 T6278 pobj seconds,for
R3830 T6281 T6277 punct ", ",°C
R3831 T6282 T6283 nummod 58,°C
R3832 T6283 T6277 conj °C,°C
R3833 T6284 T6283 prep for,°C
R3834 T6285 T6286 nummod 30,seconds
R3835 T6286 T6284 pobj seconds,for
R3836 T6287 T6283 punct ", ",°C
R3837 T6288 T6283 cc and,°C
R3838 T6289 T6290 nummod 72,°C
R3839 T6290 T6283 conj °C,°C
R3840 T6291 T6290 prep for,°C
R3841 T6292 T6293 nummod 35,seconds
R3842 T6293 T6291 pobj seconds,for
R3843 T6294 T6277 punct ", ",°C
R3844 T6295 T6277 acl repeated,°C
R3845 T6296 T6295 prep for,repeated
R3846 T6297 T6298 nummod 34,cycles
R3847 T6298 T6296 pobj cycles,for
R3848 T6299 T6275 punct .,were
R3849 T6301 T6302 amod Total,isolation
R3850 T6302 T6304 nsubjpass isolation,described
R3851 T6303 T6302 compound RNA,isolation
R3852 T6305 T6302 cc and,isolation
R3853 T6306 T6307 compound Cln3,primers
R3854 T6307 T6302 conj primers,isolation
R3855 T6308 T6309 compound RT,PCR
R3856 T6309 T6307 compound PCR,primers
R3857 T6310 T6309 punct -,PCR
R3858 T6311 T6307 cc and,primers
R3859 T6312 T6307 conj procedures,primers
R3860 T6313 T6304 aux have,described
R3861 T6314 T6304 auxpass been,described
R3862 T6315 T6304 advmod previously,described
R3863 T6316 T6317 punct [,12
R3864 T6317 T6304 parataxis 12,described
R3865 T6318 T6317 punct ],12
R3866 T6319 T6304 punct .,described