PMC:539297 / 25821-27182 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5236 0-8 NN denotes Antibody
T5238 9-12 CC denotes and
T5239 13-17 NN denotes cell
T5240 18-26 NN denotes staining
T5237 27-35 NNS denotes reagents
T5241 35-289 sentence denotes Nestin (clone Rat 401, 2 μg/ml), Lamp 1 (clone 1D4B, 6 μg/ml), and Lamp 2 (clone Abl-93; 6 μg/ml) monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, maintained by The University of Iowa, Department of Biological Sciences.
T5242 36-42 NN denotes Nestin
T5244 43-44 -LRB- denotes (
T5246 44-49 NN denotes clone
T5247 50-53 NN denotes Rat
T5248 54-57 CD denotes 401
T5249 57-59 , denotes ,
T5250 59-60 CD denotes 2
T5245 61-63 NN denotes μg
T5251 63-64 SYM denotes /
T5252 64-66 NN denotes ml
T5253 66-67 -RRB- denotes )
T5254 67-69 , denotes ,
T5255 69-73 NN denotes Lamp
T5256 74-75 CD denotes 1
T5257 76-77 -LRB- denotes (
T5259 77-82 NN denotes clone
T5260 83-87 NN denotes 1D4B
T5261 87-89 , denotes ,
T5262 89-90 CD denotes 6
T5258 91-93 NN denotes μg
T5263 93-94 SYM denotes /
T5264 94-96 NN denotes ml
T5265 96-97 -RRB- denotes )
T5266 97-99 , denotes ,
T5267 99-102 CC denotes and
T5268 103-107 NN denotes Lamp
T5269 108-109 CD denotes 2
T5270 110-111 -LRB- denotes (
T5272 111-116 NN denotes clone
T5273 117-120 NN denotes Abl
T5274 120-121 HYPH denotes -
T5275 121-123 CD denotes 93
T5276 123-124 : denotes ;
T5277 125-126 CD denotes 6
T5271 127-129 NN denotes μg
T5278 129-130 SYM denotes /
T5279 130-132 NN denotes ml
T5280 132-133 -RRB- denotes )
T5281 134-144 JJ denotes monoclonal
T5243 145-155 NNS denotes antibodies
T5283 156-160 VBD denotes were
T5282 161-169 VBN denotes obtained
T5284 170-174 IN denotes from
T5285 175-178 DT denotes the
T5287 179-192 NNP denotes Developmental
T5288 193-200 NNPS denotes Studies
T5289 201-210 NNP denotes Hybridoma
T5286 211-215 NNP denotes Bank
T5290 215-217 , denotes ,
T5291 217-227 VBN denotes maintained
T5292 228-230 IN denotes by
T5293 231-234 DT denotes The
T5294 235-245 NNP denotes University
T5296 246-248 IN denotes of
T5297 249-253 NNP denotes Iowa
T5298 253-255 , denotes ,
T5295 255-265 NNP denotes Department
T5299 266-268 IN denotes of
T5300 269-279 NNP denotes Biological
T5301 280-288 NNPS denotes Sciences
T5302 288-289 . denotes .
T5303 289-336 sentence denotes Batp1 (1 μg/ml) was previously described [12].
T5304 290-295 NN denotes Batp1
T5306 296-297 -LRB- denotes (
T5308 297-298 CD denotes 1
T5307 299-301 NN denotes μg
T5309 301-302 SYM denotes /
T5310 302-304 NN denotes ml
T5311 304-305 -RRB- denotes )
T5312 306-309 VBD denotes was
T5313 310-320 RB denotes previously
T5305 321-330 VBN denotes described
T5314 331-332 -LRB- denotes [
T5315 332-334 CD denotes 12
T5316 334-335 -RRB- denotes ]
T5317 335-336 . denotes .
T5318 336-445 sentence denotes Anti-subunit c antibody (0.7 μg/ml) was kindly provided by Dr. Kominami (Juntendo University, Tokyo, Japan).
T5319 337-349 JJ denotes Anti-subunit
T5320 350-351 NN denotes c
T5321 352-360 NN denotes antibody
T5323 361-362 -LRB- denotes (
T5325 362-365 CD denotes 0.7
T5324 366-368 NN denotes μg
T5326 368-369 SYM denotes /
T5327 369-371 NN denotes ml
T5328 371-372 -RRB- denotes )
T5329 373-376 VBD denotes was
T5330 377-383 RB denotes kindly
T5322 384-392 VBN denotes provided
T5331 393-395 IN denotes by
T5332 396-399 NNP denotes Dr.
T5333 400-408 NNP denotes Kominami
T5334 409-410 -LRB- denotes (
T5336 410-418 NNP denotes Juntendo
T5335 419-429 NNP denotes University
T5337 429-431 , denotes ,
T5338 431-436 NNP denotes Tokyo
T5339 436-438 , denotes ,
T5340 438-443 NNP denotes Japan
T5341 443-444 -RRB- denotes )
T5342 444-445 . denotes .
T5343 445-1018 sentence denotes Additional antibodies were as follows: GFAP, 1:2000 (DAKO Corporation); calbindin, 1:5000 (Sigma); NeuN, 10 μg/ml (Chemicon); SV40 T antigen (Pab 101), 2 μg/ml (Santa Cruz Biotechnology); cathepsin D (C-20), 2 μg/ml (Santa Cruz Biotechnology); cytochrome c oxidase subunit IV (cox4), 1:1000 (BD Biosciences Clontech); PDI (H-160), 2 μg/ml (Santa Cruz Biotechnology); GM130, 1 μg/ml (BD Transduction Labs); α-tubulin, 1:15,000 (Sigma); grp75, 1:200 (Stressgen); early endosome antigen-1 (EEA1), 2 μg/ml (Santa Cruz Biotechnology); rab 7, 4 μg/ml (Santa Cruz Biotechnology).
T5344 446-456 JJ denotes Additional
T5345 457-467 NNS denotes antibodies
T5346 468-472 VBD denotes were
T5347 473-475 IN denotes as
T5348 476-483 VBZ denotes follows
T5349 483-485 : denotes :
T5350 485-489 NN denotes GFAP
T5351 489-491 , denotes ,
T5352 491-492 CD denotes 1
T5353 492-493 SYM denotes :
T5354 493-497 CD denotes 2000
T5355 498-499 -LRB- denotes (
T5357 499-503 NN denotes DAKO
T5356 504-515 NN denotes Corporation
T5358 515-516 -RRB- denotes )
T5359 516-517 : denotes ;
T5360 518-527 NN denotes calbindin
T5361 527-529 , denotes ,
T5362 529-530 CD denotes 1
T5363 530-531 SYM denotes :
T5364 531-535 CD denotes 5000
T5365 536-537 -LRB- denotes (
T5366 537-542 NNP denotes Sigma
T5367 542-543 -RRB- denotes )
T5368 543-544 : denotes ;
T5369 545-549 NN denotes NeuN
T5370 549-551 , denotes ,
T5371 551-553 CD denotes 10
T5372 554-556 NN denotes μg
T5373 556-557 SYM denotes /
T5374 557-559 NN denotes ml
T5375 560-561 -LRB- denotes (
T5376 561-569 NNP denotes Chemicon
T5377 569-570 -RRB- denotes )
T5378 570-571 : denotes ;
T5379 572-576 NN denotes SV40
T5381 577-578 NN denotes T
T5380 579-586 NN denotes antigen
T5382 587-588 -LRB- denotes (
T5383 588-591 NN denotes Pab
T5384 592-595 CD denotes 101
T5385 595-596 -RRB- denotes )
T5386 596-598 , denotes ,
T5387 598-599 CD denotes 2
T5388 600-602 NN denotes μg
T5389 602-603 SYM denotes /
T5390 603-605 NN denotes ml
T5391 606-607 -LRB- denotes (
T5393 607-612 NNP denotes Santa
T5394 613-617 NNP denotes Cruz
T5392 618-631 NNP denotes Biotechnology
T5395 631-632 -RRB- denotes )
T5396 632-633 : denotes ;
T5397 634-643 NN denotes cathepsin
T5398 644-645 NN denotes D
T5399 646-647 -LRB- denotes (
T5400 647-648 NN denotes C
T5401 648-649 HYPH denotes -
T5402 649-651 CD denotes 20
T5403 651-652 -RRB- denotes )
T5404 652-654 , denotes ,
T5405 654-655 CD denotes 2
T5406 656-658 NN denotes μg
T5407 658-659 SYM denotes /
T5408 659-661 NN denotes ml
T5409 662-663 -LRB- denotes (
T5411 663-668 NNP denotes Santa
T5412 669-673 NNP denotes Cruz
T5410 674-687 NNP denotes Biotechnology
T5413 687-688 -RRB- denotes )
T5414 688-689 : denotes ;
T5415 690-700 NN denotes cytochrome
T5417 701-702 NN denotes c
T5418 703-710 NN denotes oxidase
T5416 711-718 NN denotes subunit
T5419 719-721 CD denotes IV
T5420 722-723 -LRB- denotes (
T5421 723-727 NN denotes cox4
T5422 727-728 -RRB- denotes )
T5423 728-730 , denotes ,
T5424 730-731 CD denotes 1
T5425 731-732 SYM denotes :
T5426 732-736 CD denotes 1000
T5427 737-738 -LRB- denotes (
T5429 738-740 NN denotes BD
T5430 741-752 NNP denotes Biosciences
T5428 753-761 NNP denotes Clontech
T5431 761-762 -RRB- denotes )
T5432 762-763 : denotes ;
T5433 764-767 NN denotes PDI
T5434 768-769 -LRB- denotes (
T5435 769-770 NN denotes H
T5436 770-771 HYPH denotes -
T5437 771-774 CD denotes 160
T5438 774-775 -RRB- denotes )
T5439 775-777 , denotes ,
T5440 777-778 CD denotes 2
T5441 779-781 NN denotes μg
T5442 781-782 SYM denotes /
T5443 782-784 NN denotes ml
T5444 785-786 -LRB- denotes (
T5446 786-791 NNP denotes Santa
T5447 792-796 NNP denotes Cruz
T5445 797-810 NNP denotes Biotechnology
T5448 810-811 -RRB- denotes )
T5449 811-812 : denotes ;
T5450 813-818 NN denotes GM130
T5451 818-820 , denotes ,
T5452 820-821 CD denotes 1
T5453 822-824 NN denotes μg
T5454 824-825 SYM denotes /
T5455 825-827 NN denotes ml
T5456 828-829 -LRB- denotes (
T5458 829-831 NN denotes BD
T5459 832-844 NN denotes Transduction
T5457 845-849 NNS denotes Labs
T5460 849-850 -RRB- denotes )
T5461 850-851 : denotes ;
T5462 852-853 NN denotes α
T5464 853-854 HYPH denotes -
T5463 854-861 NN denotes tubulin
T5465 861-863 , denotes ,
T5466 863-864 CD denotes 1
T5467 864-865 SYM denotes :
T5468 865-871 CD denotes 15,000
T5469 872-873 -LRB- denotes (
T5470 873-878 NNP denotes Sigma
T5471 878-879 -RRB- denotes )
T5472 879-880 : denotes ;
T5473 881-886 NN denotes grp75
T5474 886-888 , denotes ,
T5475 888-889 CD denotes 1
T5476 889-890 SYM denotes :
T5477 890-893 CD denotes 200
T5478 894-895 -LRB- denotes (
T5479 895-904 NNP denotes Stressgen
T5480 904-905 -RRB- denotes )
T5481 905-906 : denotes ;
T5482 907-912 JJ denotes early
T5484 913-921 NN denotes endosome
T5483 922-929 NN denotes antigen
T5485 929-930 HYPH denotes -
T5486 930-931 CD denotes 1
T5487 932-933 -LRB- denotes (
T5488 933-937 NN denotes EEA1
T5489 937-938 -RRB- denotes )
T5490 938-940 , denotes ,
T5491 940-941 CD denotes 2
T5492 942-944 NN denotes μg
T5493 944-945 SYM denotes /
T5494 945-947 NN denotes ml
T5495 948-949 -LRB- denotes (
T5497 949-954 NNP denotes Santa
T5498 955-959 NNP denotes Cruz
T5496 960-973 NNP denotes Biotechnology
T5499 973-974 -RRB- denotes )
T5500 974-975 : denotes ;
T5501 976-979 NN denotes rab
T5502 980-981 CD denotes 7
T5503 981-983 , denotes ,
T5504 983-984 CD denotes 4
T5505 985-987 NN denotes μg
T5506 987-988 SYM denotes /
T5507 988-990 NN denotes ml
T5508 991-992 -LRB- denotes (
T5510 992-997 NNP denotes Santa
T5511 998-1002 NNP denotes Cruz
T5509 1003-1016 NNP denotes Biotechnology
T5512 1016-1017 -RRB- denotes )
T5513 1017-1018 . denotes .
T5514 1018-1191 sentence denotes All fluorescent secondary antibodies were obtained from Jackson ImmunoResearch Laboratories and HRP-conjugated secondary antibodies were obtained from Amersham Biosciences.
T5515 1019-1022 DT denotes All
T5517 1023-1034 JJ denotes fluorescent
T5518 1035-1044 JJ denotes secondary
T5516 1045-1055 NNS denotes antibodies
T5520 1056-1060 VBD denotes were
T5519 1061-1069 VBN denotes obtained
T5521 1070-1074 IN denotes from
T5522 1075-1082 NNP denotes Jackson
T5524 1083-1097 NNP denotes ImmunoResearch
T5523 1098-1110 NNP denotes Laboratories
T5525 1111-1114 CC denotes and
T5526 1115-1118 NN denotes HRP
T5528 1118-1119 HYPH denotes -
T5527 1119-1129 VBN denotes conjugated
T5530 1130-1139 JJ denotes secondary
T5529 1140-1150 NNS denotes antibodies
T5532 1151-1155 VBD denotes were
T5531 1156-1164 VBN denotes obtained
T5533 1165-1169 IN denotes from
T5534 1170-1178 NNP denotes Amersham
T5535 1179-1190 NNP denotes Biosciences
T5536 1190-1191 . denotes .
T5537 1191-1361 sentence denotes Additional cell markers were as follows: VVL-biotin, 1:2000 (Vector Laboratories), 10,000 MW dextran-FITC, 1 mg/ml and Lysotracker Red DND-99, 500 nM (Molecular Probes).
T5538 1192-1202 JJ denotes Additional
T5540 1203-1207 NN denotes cell
T5539 1208-1215 NNS denotes markers
T5541 1216-1220 VBD denotes were
T5542 1221-1223 IN denotes as
T5543 1224-1231 VBZ denotes follows
T5544 1231-1233 : denotes :
T5545 1233-1236 NN denotes VVL
T5547 1236-1237 HYPH denotes -
T5546 1237-1243 NN denotes biotin
T5548 1243-1245 , denotes ,
T5549 1245-1246 CD denotes 1
T5550 1246-1247 SYM denotes :
T5551 1247-1251 CD denotes 2000
T5552 1252-1253 -LRB- denotes (
T5554 1253-1259 NNP denotes Vector
T5553 1260-1272 NNP denotes Laboratories
T5555 1272-1273 -RRB- denotes )
T5556 1273-1275 , denotes ,
T5557 1275-1281 CD denotes 10,000
T5558 1282-1284 NN denotes MW
T5560 1285-1292 NN denotes dextran
T5561 1292-1293 HYPH denotes -
T5559 1293-1297 NN denotes FITC
T5562 1297-1299 , denotes ,
T5563 1299-1300 CD denotes 1
T5564 1301-1303 NN denotes mg
T5565 1303-1304 SYM denotes /
T5566 1304-1306 NN denotes ml
T5567 1307-1310 CC denotes and
T5568 1311-1322 NNP denotes Lysotracker
T5570 1323-1326 NN denotes Red
T5569 1327-1330 NN denotes DND
T5571 1330-1331 HYPH denotes -
T5572 1331-1333 CD denotes 99
T5573 1333-1335 , denotes ,
T5574 1335-1338 CD denotes 500
T5575 1339-1341 NN denotes nM
T5576 1342-1343 -LRB- denotes (
T5578 1343-1352 NNP denotes Molecular
T5577 1353-1359 NNP denotes Probes
T5579 1359-1360 -RRB- denotes )
T5580 1360-1361 . denotes .
R3000 T5236 T5237 nmod Antibody,reagents
R3001 T5238 T5236 cc and,Antibody
R3002 T5239 T5240 compound cell,staining
R3003 T5240 T5236 conj staining,Antibody
R3004 T5242 T5243 nmod Nestin,antibodies
R3005 T5243 T5282 nsubjpass antibodies,obtained
R3006 T5244 T5245 punct (,μg
R3007 T5245 T5242 parataxis μg,Nestin
R3008 T5246 T5247 compound clone,Rat
R3009 T5247 T5245 dep Rat,μg
R3010 T5248 T5247 nummod 401,Rat
R3011 T5249 T5245 punct ", ",μg
R3012 T5250 T5245 nummod 2,μg
R3013 T5251 T5252 punct /,ml
R3014 T5252 T5245 prep ml,μg
R3015 T5253 T5245 punct ),μg
R3016 T5254 T5242 punct ", ",Nestin
R3017 T5255 T5242 conj Lamp,Nestin
R3018 T5256 T5255 nummod 1,Lamp
R3019 T5257 T5258 punct (,μg
R3020 T5258 T5255 parataxis μg,Lamp
R3021 T5259 T5260 compound clone,1D4B
R3022 T5260 T5258 dep 1D4B,μg
R3023 T5261 T5258 punct ", ",μg
R3024 T5262 T5258 nummod 6,μg
R3025 T5263 T5264 punct /,ml
R3026 T5264 T5258 prep ml,μg
R3027 T5265 T5258 punct ),μg
R3028 T5266 T5255 punct ", ",Lamp
R3029 T5267 T5255 cc and,Lamp
R3030 T5268 T5255 conj Lamp,Lamp
R3031 T5269 T5268 nummod 2,Lamp
R3032 T5270 T5271 punct (,μg
R3033 T5271 T5268 parataxis μg,Lamp
R3034 T5272 T5273 compound clone,Abl
R3035 T5273 T5271 dep Abl,μg
R3036 T5274 T5273 punct -,Abl
R3037 T5275 T5273 nummod 93,Abl
R3038 T5276 T5271 punct ;,μg
R3039 T5277 T5271 nummod 6,μg
R3040 T5278 T5279 punct /,ml
R3041 T5279 T5271 prep ml,μg
R3042 T5280 T5271 punct ),μg
R3043 T5281 T5243 amod monoclonal,antibodies
R3044 T5283 T5282 auxpass were,obtained
R3045 T5284 T5282 prep from,obtained
R3046 T5285 T5286 det the,Bank
R3047 T5286 T5284 pobj Bank,from
R3048 T5287 T5286 compound Developmental,Bank
R3049 T5288 T5286 compound Studies,Bank
R3050 T5289 T5286 compound Hybridoma,Bank
R3051 T5290 T5286 punct ", ",Bank
R3052 T5291 T5286 acl maintained,Bank
R3053 T5292 T5291 agent by,maintained
R3054 T5293 T5294 det The,University
R3055 T5294 T5295 nmod University,Department
R3056 T5295 T5292 pobj Department,by
R3057 T5296 T5294 prep of,University
R3058 T5297 T5296 pobj Iowa,of
R3059 T5298 T5295 punct ", ",Department
R3060 T5299 T5295 prep of,Department
R3061 T5300 T5301 compound Biological,Sciences
R3062 T5301 T5299 pobj Sciences,of
R3063 T5302 T5282 punct .,obtained
R3064 T5304 T5305 nsubjpass Batp1,described
R3065 T5306 T5307 punct (,μg
R3066 T5307 T5304 parataxis μg,Batp1
R3067 T5308 T5307 nummod 1,μg
R3068 T5309 T5310 punct /,ml
R3069 T5310 T5307 prep ml,μg
R3070 T5311 T5307 punct ),μg
R3071 T5312 T5305 auxpass was,described
R3072 T5313 T5305 advmod previously,described
R3073 T5314 T5315 punct [,12
R3074 T5315 T5305 parataxis 12,described
R3075 T5316 T5315 punct ],12
R3076 T5317 T5305 punct .,described
R3077 T5319 T5320 amod Anti-subunit,c
R3078 T5320 T5321 amod c,antibody
R3079 T5321 T5322 nsubjpass antibody,provided
R3080 T5323 T5324 punct (,μg
R3081 T5324 T5321 parataxis μg,antibody
R3082 T5325 T5324 nummod 0.7,μg
R3083 T5326 T5327 punct /,ml
R3084 T5327 T5324 prep ml,μg
R3085 T5328 T5324 punct ),μg
R3086 T5329 T5322 auxpass was,provided
R3087 T5330 T5322 advmod kindly,provided
R3088 T5331 T5322 agent by,provided
R3089 T5332 T5333 compound Dr.,Kominami
R3090 T5333 T5331 pobj Kominami,by
R3091 T5334 T5335 punct (,University
R3092 T5335 T5333 parataxis University,Kominami
R3093 T5336 T5335 compound Juntendo,University
R3094 T5337 T5335 punct ", ",University
R3095 T5338 T5335 npadvmod Tokyo,University
R3096 T5339 T5335 punct ", ",University
R3097 T5340 T5335 npadvmod Japan,University
R3098 T5341 T5335 punct ),University
R3099 T5342 T5322 punct .,provided
R3100 T5344 T5345 amod Additional,antibodies
R3101 T5345 T5346 nsubj antibodies,were
R3102 T5347 T5348 mark as,follows
R3103 T5348 T5346 advcl follows,were
R3104 T5349 T5346 punct : ,were
R3105 T5350 T5346 dep GFAP,were
R3106 T5351 T5350 punct ", ",GFAP
R3107 T5352 T5350 npadvmod 1,GFAP
R3108 T5353 T5354 punct :,2000
R3109 T5354 T5352 prep 2000,1
R3110 T5355 T5356 punct (,Corporation
R3111 T5356 T5350 parataxis Corporation,GFAP
R3112 T5357 T5356 compound DAKO,Corporation
R3113 T5358 T5356 punct ),Corporation
R3114 T5359 T5350 punct ;,GFAP
R3115 T5360 T5350 appos calbindin,GFAP
R3116 T5361 T5360 punct ", ",calbindin
R3117 T5362 T5360 npadvmod 1,calbindin
R3118 T5363 T5364 punct :,5000
R3119 T5364 T5362 prep 5000,1
R3120 T5365 T5366 punct (,Sigma
R3121 T5366 T5360 parataxis Sigma,calbindin
R3122 T5367 T5366 punct ),Sigma
R3123 T5368 T5350 punct ;,GFAP
R3124 T5369 T5350 appos NeuN,GFAP
R3125 T5370 T5369 punct ", ",NeuN
R3126 T5371 T5372 nummod 10,μg
R3127 T5372 T5369 npadvmod μg,NeuN
R3128 T5373 T5374 punct /,ml
R3129 T5374 T5372 prep ml,μg
R3130 T5375 T5376 punct (,Chemicon
R3131 T5376 T5369 parataxis Chemicon,NeuN
R3132 T5377 T5376 punct ),Chemicon
R3133 T5378 T5350 punct ;,GFAP
R3134 T5379 T5380 compound SV40,antigen
R3135 T5380 T5350 appos antigen,GFAP
R3136 T5381 T5380 compound T,antigen
R3137 T5382 T5380 punct (,antigen
R3138 T5383 T5380 appos Pab,antigen
R3139 T5384 T5383 nummod 101,Pab
R3140 T5385 T5380 punct ),antigen
R3141 T5386 T5380 punct ", ",antigen
R3142 T5387 T5388 nummod 2,μg
R3143 T5388 T5380 npadvmod μg,antigen
R3144 T5389 T5390 punct /,ml
R3145 T5390 T5388 prep ml,μg
R3146 T5391 T5392 punct (,Biotechnology
R3147 T5392 T5380 parataxis Biotechnology,antigen
R3148 T5393 T5392 compound Santa,Biotechnology
R3149 T5394 T5392 compound Cruz,Biotechnology
R3150 T5395 T5392 punct ),Biotechnology
R3151 T5396 T5350 punct ;,GFAP
R3152 T5397 T5398 compound cathepsin,D
R3153 T5398 T5350 appos D,GFAP
R3154 T5399 T5398 punct (,D
R3155 T5400 T5398 appos C,D
R3156 T5401 T5400 punct -,C
R3157 T5402 T5400 nummod 20,C
R3158 T5403 T5398 punct ),D
R3159 T5404 T5398 punct ", ",D
R3160 T5405 T5406 nummod 2,μg
R3161 T5406 T5398 npadvmod μg,D
R3162 T5407 T5408 punct /,ml
R3163 T5408 T5406 prep ml,μg
R3164 T5409 T5410 punct (,Biotechnology
R3165 T5410 T5398 parataxis Biotechnology,D
R3166 T5411 T5410 compound Santa,Biotechnology
R3167 T5412 T5410 compound Cruz,Biotechnology
R3168 T5413 T5410 punct ),Biotechnology
R3169 T5414 T5350 punct ;,GFAP
R3170 T5415 T5416 compound cytochrome,subunit
R3171 T5416 T5350 appos subunit,GFAP
R3172 T5417 T5416 compound c,subunit
R3173 T5418 T5416 compound oxidase,subunit
R3174 T5419 T5416 nummod IV,subunit
R3175 T5420 T5416 punct (,subunit
R3176 T5421 T5416 appos cox4,subunit
R3177 T5422 T5416 punct ),subunit
R3178 T5423 T5416 punct ", ",subunit
R3179 T5424 T5416 npadvmod 1,subunit
R3180 T5425 T5426 punct :,1000
R3181 T5426 T5424 prep 1000,1
R3182 T5427 T5428 punct (,Clontech
R3183 T5428 T5416 parataxis Clontech,subunit
R3184 T5429 T5428 compound BD,Clontech
R3185 T5430 T5428 compound Biosciences,Clontech
R3186 T5431 T5428 punct ),Clontech
R3187 T5432 T5350 punct ;,GFAP
R3188 T5433 T5350 appos PDI,GFAP
R3189 T5434 T5433 punct (,PDI
R3190 T5435 T5433 appos H,PDI
R3191 T5436 T5435 punct -,H
R3192 T5437 T5435 nummod 160,H
R3193 T5438 T5433 punct ),PDI
R3194 T5439 T5433 punct ", ",PDI
R3195 T5440 T5441 nummod 2,μg
R3196 T5441 T5433 npadvmod μg,PDI
R3197 T5442 T5443 punct /,ml
R3198 T5443 T5441 prep ml,μg
R3199 T5444 T5445 punct (,Biotechnology
R3200 T5445 T5433 parataxis Biotechnology,PDI
R3201 T5446 T5445 compound Santa,Biotechnology
R3202 T5447 T5445 compound Cruz,Biotechnology
R3203 T5448 T5445 punct ),Biotechnology
R3204 T5449 T5350 punct ;,GFAP
R3205 T5450 T5350 appos GM130,GFAP
R3206 T5451 T5450 punct ", ",GM130
R3207 T5452 T5453 nummod 1,μg
R3208 T5453 T5450 npadvmod μg,GM130
R3209 T5454 T5455 punct /,ml
R3210 T5455 T5453 prep ml,μg
R3211 T5456 T5457 punct (,Labs
R3212 T5457 T5450 parataxis Labs,GM130
R3213 T5458 T5457 compound BD,Labs
R3214 T5459 T5457 compound Transduction,Labs
R3215 T5460 T5457 punct ),Labs
R3216 T5461 T5350 punct ;,GFAP
R3217 T5462 T5463 compound α,tubulin
R3218 T5463 T5350 appos tubulin,GFAP
R3219 T5464 T5463 punct -,tubulin
R3220 T5465 T5463 punct ", ",tubulin
R3221 T5466 T5463 npadvmod 1,tubulin
R3222 T5467 T5468 punct :,"15,000"
R3223 T5468 T5466 prep "15,000",1
R3224 T5469 T5470 punct (,Sigma
R3225 T5470 T5463 parataxis Sigma,tubulin
R3226 T5471 T5470 punct ),Sigma
R3227 T5472 T5350 punct ;,GFAP
R3228 T5473 T5350 appos grp75,GFAP
R3229 T5474 T5473 punct ", ",grp75
R3230 T5475 T5473 npadvmod 1,grp75
R3231 T5476 T5477 punct :,200
R3232 T5477 T5475 prep 200,1
R3233 T5478 T5479 punct (,Stressgen
R3234 T5479 T5473 parataxis Stressgen,grp75
R3235 T5480 T5479 punct ),Stressgen
R3236 T5481 T5350 punct ;,GFAP
R3237 T5482 T5483 amod early,antigen
R3238 T5483 T5350 appos antigen,GFAP
R3239 T5484 T5483 compound endosome,antigen
R3240 T5485 T5483 punct -,antigen
R3241 T5486 T5483 nummod 1,antigen
R3242 T5487 T5483 punct (,antigen
R3243 T5488 T5483 appos EEA1,antigen
R3244 T5489 T5483 punct ),antigen
R3245 T5490 T5483 punct ", ",antigen
R3246 T5491 T5492 nummod 2,μg
R3247 T5492 T5483 npadvmod μg,antigen
R3248 T5493 T5494 punct /,ml
R3249 T5494 T5492 prep ml,μg
R3250 T5495 T5496 punct (,Biotechnology
R3251 T5496 T5483 parataxis Biotechnology,antigen
R3252 T5497 T5496 compound Santa,Biotechnology
R3253 T5498 T5496 compound Cruz,Biotechnology
R3254 T5499 T5496 punct ),Biotechnology
R3255 T5500 T5350 punct ;,GFAP
R3256 T5501 T5350 appos rab,GFAP
R3257 T5502 T5501 nummod 7,rab
R3258 T5503 T5501 punct ", ",rab
R3259 T5504 T5505 nummod 4,μg
R3260 T5505 T5501 npadvmod μg,rab
R3261 T5506 T5507 punct /,ml
R3262 T5507 T5505 prep ml,μg
R3263 T5508 T5509 punct (,Biotechnology
R3264 T5509 T5501 parataxis Biotechnology,rab
R3265 T5510 T5509 compound Santa,Biotechnology
R3266 T5511 T5509 compound Cruz,Biotechnology
R3267 T5512 T5509 punct ),Biotechnology
R3268 T5513 T5346 punct .,were
R3269 T5515 T5516 det All,antibodies
R3270 T5516 T5519 nsubjpass antibodies,obtained
R3271 T5517 T5516 amod fluorescent,antibodies
R3272 T5518 T5516 amod secondary,antibodies
R3273 T5520 T5519 auxpass were,obtained
R3274 T5521 T5519 prep from,obtained
R3275 T5522 T5523 compound Jackson,Laboratories
R3276 T5523 T5521 pobj Laboratories,from
R3277 T5524 T5523 compound ImmunoResearch,Laboratories
R3278 T5525 T5519 cc and,obtained
R3279 T5526 T5527 npadvmod HRP,conjugated
R3280 T5527 T5529 amod conjugated,antibodies
R3281 T5528 T5527 punct -,conjugated
R3282 T5529 T5531 nsubjpass antibodies,obtained
R3283 T5530 T5529 amod secondary,antibodies
R3284 T5531 T5519 conj obtained,obtained
R3285 T5532 T5531 auxpass were,obtained
R3286 T5533 T5531 prep from,obtained
R3287 T5534 T5535 compound Amersham,Biosciences
R3288 T5535 T5533 pobj Biosciences,from
R3289 T5536 T5531 punct .,obtained
R3290 T5538 T5539 amod Additional,markers
R3291 T5539 T5541 nsubj markers,were
R3292 T5540 T5539 compound cell,markers
R3293 T5542 T5543 mark as,follows
R3294 T5543 T5541 advcl follows,were
R3295 T5544 T5541 punct : ,were
R3296 T5545 T5546 compound VVL,biotin
R3297 T5546 T5541 dep biotin,were
R3298 T5547 T5546 punct -,biotin
R3299 T5548 T5546 punct ", ",biotin
R3300 T5549 T5546 npadvmod 1,biotin
R3301 T5550 T5551 punct :,2000
R3302 T5551 T5549 prep 2000,1
R3303 T5552 T5553 punct (,Laboratories
R3304 T5553 T5546 parataxis Laboratories,biotin
R3305 T5554 T5553 compound Vector,Laboratories
R3306 T5555 T5553 punct ),Laboratories
R3307 T5556 T5546 punct ", ",biotin
R3308 T5557 T5558 nummod "10,000",MW
R3309 T5558 T5559 compound MW,FITC
R3310 T5559 T5546 conj FITC,biotin
R3311 T5560 T5559 compound dextran,FITC
R3312 T5561 T5559 punct -,FITC
R3313 T5562 T5559 punct ", ",FITC
R3314 T5563 T5564 nummod 1,mg
R3315 T5564 T5559 npadvmod mg,FITC
R3316 T5565 T5566 punct /,ml
R3317 T5566 T5564 prep ml,mg
R3318 T5567 T5559 cc and,FITC
R3319 T5568 T5569 compound Lysotracker,DND
R3320 T5569 T5559 conj DND,FITC
R3321 T5570 T5569 compound Red,DND
R3322 T5571 T5569 punct -,DND
R3323 T5572 T5569 nummod 99,DND
R3324 T5573 T5569 punct ", ",DND
R3325 T5574 T5575 nummod 500,nM
R3326 T5575 T5569 npadvmod nM,DND
R3327 T5576 T5577 punct (,Probes
R3328 T5577 T5569 parataxis Probes,DND
R3329 T5578 T5577 compound Molecular,Probes
R3330 T5579 T5577 punct ),Probes
R3331 T5580 T5541 punct .,were