Id |
Subject |
Object |
Predicate |
Lexical cue |
T5236 |
0-8 |
NN |
denotes |
Antibody |
T5238 |
9-12 |
CC |
denotes |
and |
T5239 |
13-17 |
NN |
denotes |
cell |
T5240 |
18-26 |
NN |
denotes |
staining |
T5237 |
27-35 |
NNS |
denotes |
reagents |
T5241 |
35-289 |
sentence |
denotes |
Nestin (clone Rat 401, 2 μg/ml), Lamp 1 (clone 1D4B, 6 μg/ml), and Lamp 2 (clone Abl-93; 6 μg/ml) monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, maintained by The University of Iowa, Department of Biological Sciences. |
T5242 |
36-42 |
NN |
denotes |
Nestin |
T5244 |
43-44 |
-LRB- |
denotes |
( |
T5246 |
44-49 |
NN |
denotes |
clone |
T5247 |
50-53 |
NN |
denotes |
Rat |
T5248 |
54-57 |
CD |
denotes |
401 |
T5249 |
57-59 |
, |
denotes |
, |
T5250 |
59-60 |
CD |
denotes |
2 |
T5245 |
61-63 |
NN |
denotes |
μg |
T5251 |
63-64 |
SYM |
denotes |
/ |
T5252 |
64-66 |
NN |
denotes |
ml |
T5253 |
66-67 |
-RRB- |
denotes |
) |
T5254 |
67-69 |
, |
denotes |
, |
T5255 |
69-73 |
NN |
denotes |
Lamp |
T5256 |
74-75 |
CD |
denotes |
1 |
T5257 |
76-77 |
-LRB- |
denotes |
( |
T5259 |
77-82 |
NN |
denotes |
clone |
T5260 |
83-87 |
NN |
denotes |
1D4B |
T5261 |
87-89 |
, |
denotes |
, |
T5262 |
89-90 |
CD |
denotes |
6 |
T5258 |
91-93 |
NN |
denotes |
μg |
T5263 |
93-94 |
SYM |
denotes |
/ |
T5264 |
94-96 |
NN |
denotes |
ml |
T5265 |
96-97 |
-RRB- |
denotes |
) |
T5266 |
97-99 |
, |
denotes |
, |
T5267 |
99-102 |
CC |
denotes |
and |
T5268 |
103-107 |
NN |
denotes |
Lamp |
T5269 |
108-109 |
CD |
denotes |
2 |
T5270 |
110-111 |
-LRB- |
denotes |
( |
T5272 |
111-116 |
NN |
denotes |
clone |
T5273 |
117-120 |
NN |
denotes |
Abl |
T5274 |
120-121 |
HYPH |
denotes |
- |
T5275 |
121-123 |
CD |
denotes |
93 |
T5276 |
123-124 |
: |
denotes |
; |
T5277 |
125-126 |
CD |
denotes |
6 |
T5271 |
127-129 |
NN |
denotes |
μg |
T5278 |
129-130 |
SYM |
denotes |
/ |
T5279 |
130-132 |
NN |
denotes |
ml |
T5280 |
132-133 |
-RRB- |
denotes |
) |
T5281 |
134-144 |
JJ |
denotes |
monoclonal |
T5243 |
145-155 |
NNS |
denotes |
antibodies |
T5283 |
156-160 |
VBD |
denotes |
were |
T5282 |
161-169 |
VBN |
denotes |
obtained |
T5284 |
170-174 |
IN |
denotes |
from |
T5285 |
175-178 |
DT |
denotes |
the |
T5287 |
179-192 |
NNP |
denotes |
Developmental |
T5288 |
193-200 |
NNPS |
denotes |
Studies |
T5289 |
201-210 |
NNP |
denotes |
Hybridoma |
T5286 |
211-215 |
NNP |
denotes |
Bank |
T5290 |
215-217 |
, |
denotes |
, |
T5291 |
217-227 |
VBN |
denotes |
maintained |
T5292 |
228-230 |
IN |
denotes |
by |
T5293 |
231-234 |
DT |
denotes |
The |
T5294 |
235-245 |
NNP |
denotes |
University |
T5296 |
246-248 |
IN |
denotes |
of |
T5297 |
249-253 |
NNP |
denotes |
Iowa |
T5298 |
253-255 |
, |
denotes |
, |
T5295 |
255-265 |
NNP |
denotes |
Department |
T5299 |
266-268 |
IN |
denotes |
of |
T5300 |
269-279 |
NNP |
denotes |
Biological |
T5301 |
280-288 |
NNPS |
denotes |
Sciences |
T5302 |
288-289 |
. |
denotes |
. |
T5303 |
289-336 |
sentence |
denotes |
Batp1 (1 μg/ml) was previously described [12]. |
T5304 |
290-295 |
NN |
denotes |
Batp1 |
T5306 |
296-297 |
-LRB- |
denotes |
( |
T5308 |
297-298 |
CD |
denotes |
1 |
T5307 |
299-301 |
NN |
denotes |
μg |
T5309 |
301-302 |
SYM |
denotes |
/ |
T5310 |
302-304 |
NN |
denotes |
ml |
T5311 |
304-305 |
-RRB- |
denotes |
) |
T5312 |
306-309 |
VBD |
denotes |
was |
T5313 |
310-320 |
RB |
denotes |
previously |
T5305 |
321-330 |
VBN |
denotes |
described |
T5314 |
331-332 |
-LRB- |
denotes |
[ |
T5315 |
332-334 |
CD |
denotes |
12 |
T5316 |
334-335 |
-RRB- |
denotes |
] |
T5317 |
335-336 |
. |
denotes |
. |
T5318 |
336-445 |
sentence |
denotes |
Anti-subunit c antibody (0.7 μg/ml) was kindly provided by Dr. Kominami (Juntendo University, Tokyo, Japan). |
T5319 |
337-349 |
JJ |
denotes |
Anti-subunit |
T5320 |
350-351 |
NN |
denotes |
c |
T5321 |
352-360 |
NN |
denotes |
antibody |
T5323 |
361-362 |
-LRB- |
denotes |
( |
T5325 |
362-365 |
CD |
denotes |
0.7 |
T5324 |
366-368 |
NN |
denotes |
μg |
T5326 |
368-369 |
SYM |
denotes |
/ |
T5327 |
369-371 |
NN |
denotes |
ml |
T5328 |
371-372 |
-RRB- |
denotes |
) |
T5329 |
373-376 |
VBD |
denotes |
was |
T5330 |
377-383 |
RB |
denotes |
kindly |
T5322 |
384-392 |
VBN |
denotes |
provided |
T5331 |
393-395 |
IN |
denotes |
by |
T5332 |
396-399 |
NNP |
denotes |
Dr. |
T5333 |
400-408 |
NNP |
denotes |
Kominami |
T5334 |
409-410 |
-LRB- |
denotes |
( |
T5336 |
410-418 |
NNP |
denotes |
Juntendo |
T5335 |
419-429 |
NNP |
denotes |
University |
T5337 |
429-431 |
, |
denotes |
, |
T5338 |
431-436 |
NNP |
denotes |
Tokyo |
T5339 |
436-438 |
, |
denotes |
, |
T5340 |
438-443 |
NNP |
denotes |
Japan |
T5341 |
443-444 |
-RRB- |
denotes |
) |
T5342 |
444-445 |
. |
denotes |
. |
T5343 |
445-1018 |
sentence |
denotes |
Additional antibodies were as follows: GFAP, 1:2000 (DAKO Corporation); calbindin, 1:5000 (Sigma); NeuN, 10 μg/ml (Chemicon); SV40 T antigen (Pab 101), 2 μg/ml (Santa Cruz Biotechnology); cathepsin D (C-20), 2 μg/ml (Santa Cruz Biotechnology); cytochrome c oxidase subunit IV (cox4), 1:1000 (BD Biosciences Clontech); PDI (H-160), 2 μg/ml (Santa Cruz Biotechnology); GM130, 1 μg/ml (BD Transduction Labs); α-tubulin, 1:15,000 (Sigma); grp75, 1:200 (Stressgen); early endosome antigen-1 (EEA1), 2 μg/ml (Santa Cruz Biotechnology); rab 7, 4 μg/ml (Santa Cruz Biotechnology). |
T5344 |
446-456 |
JJ |
denotes |
Additional |
T5345 |
457-467 |
NNS |
denotes |
antibodies |
T5346 |
468-472 |
VBD |
denotes |
were |
T5347 |
473-475 |
IN |
denotes |
as |
T5348 |
476-483 |
VBZ |
denotes |
follows |
T5349 |
483-485 |
: |
denotes |
: |
T5350 |
485-489 |
NN |
denotes |
GFAP |
T5351 |
489-491 |
, |
denotes |
, |
T5352 |
491-492 |
CD |
denotes |
1 |
T5353 |
492-493 |
SYM |
denotes |
: |
T5354 |
493-497 |
CD |
denotes |
2000 |
T5355 |
498-499 |
-LRB- |
denotes |
( |
T5357 |
499-503 |
NN |
denotes |
DAKO |
T5356 |
504-515 |
NN |
denotes |
Corporation |
T5358 |
515-516 |
-RRB- |
denotes |
) |
T5359 |
516-517 |
: |
denotes |
; |
T5360 |
518-527 |
NN |
denotes |
calbindin |
T5361 |
527-529 |
, |
denotes |
, |
T5362 |
529-530 |
CD |
denotes |
1 |
T5363 |
530-531 |
SYM |
denotes |
: |
T5364 |
531-535 |
CD |
denotes |
5000 |
T5365 |
536-537 |
-LRB- |
denotes |
( |
T5366 |
537-542 |
NNP |
denotes |
Sigma |
T5367 |
542-543 |
-RRB- |
denotes |
) |
T5368 |
543-544 |
: |
denotes |
; |
T5369 |
545-549 |
NN |
denotes |
NeuN |
T5370 |
549-551 |
, |
denotes |
, |
T5371 |
551-553 |
CD |
denotes |
10 |
T5372 |
554-556 |
NN |
denotes |
μg |
T5373 |
556-557 |
SYM |
denotes |
/ |
T5374 |
557-559 |
NN |
denotes |
ml |
T5375 |
560-561 |
-LRB- |
denotes |
( |
T5376 |
561-569 |
NNP |
denotes |
Chemicon |
T5377 |
569-570 |
-RRB- |
denotes |
) |
T5378 |
570-571 |
: |
denotes |
; |
T5379 |
572-576 |
NN |
denotes |
SV40 |
T5381 |
577-578 |
NN |
denotes |
T |
T5380 |
579-586 |
NN |
denotes |
antigen |
T5382 |
587-588 |
-LRB- |
denotes |
( |
T5383 |
588-591 |
NN |
denotes |
Pab |
T5384 |
592-595 |
CD |
denotes |
101 |
T5385 |
595-596 |
-RRB- |
denotes |
) |
T5386 |
596-598 |
, |
denotes |
, |
T5387 |
598-599 |
CD |
denotes |
2 |
T5388 |
600-602 |
NN |
denotes |
μg |
T5389 |
602-603 |
SYM |
denotes |
/ |
T5390 |
603-605 |
NN |
denotes |
ml |
T5391 |
606-607 |
-LRB- |
denotes |
( |
T5393 |
607-612 |
NNP |
denotes |
Santa |
T5394 |
613-617 |
NNP |
denotes |
Cruz |
T5392 |
618-631 |
NNP |
denotes |
Biotechnology |
T5395 |
631-632 |
-RRB- |
denotes |
) |
T5396 |
632-633 |
: |
denotes |
; |
T5397 |
634-643 |
NN |
denotes |
cathepsin |
T5398 |
644-645 |
NN |
denotes |
D |
T5399 |
646-647 |
-LRB- |
denotes |
( |
T5400 |
647-648 |
NN |
denotes |
C |
T5401 |
648-649 |
HYPH |
denotes |
- |
T5402 |
649-651 |
CD |
denotes |
20 |
T5403 |
651-652 |
-RRB- |
denotes |
) |
T5404 |
652-654 |
, |
denotes |
, |
T5405 |
654-655 |
CD |
denotes |
2 |
T5406 |
656-658 |
NN |
denotes |
μg |
T5407 |
658-659 |
SYM |
denotes |
/ |
T5408 |
659-661 |
NN |
denotes |
ml |
T5409 |
662-663 |
-LRB- |
denotes |
( |
T5411 |
663-668 |
NNP |
denotes |
Santa |
T5412 |
669-673 |
NNP |
denotes |
Cruz |
T5410 |
674-687 |
NNP |
denotes |
Biotechnology |
T5413 |
687-688 |
-RRB- |
denotes |
) |
T5414 |
688-689 |
: |
denotes |
; |
T5415 |
690-700 |
NN |
denotes |
cytochrome |
T5417 |
701-702 |
NN |
denotes |
c |
T5418 |
703-710 |
NN |
denotes |
oxidase |
T5416 |
711-718 |
NN |
denotes |
subunit |
T5419 |
719-721 |
CD |
denotes |
IV |
T5420 |
722-723 |
-LRB- |
denotes |
( |
T5421 |
723-727 |
NN |
denotes |
cox4 |
T5422 |
727-728 |
-RRB- |
denotes |
) |
T5423 |
728-730 |
, |
denotes |
, |
T5424 |
730-731 |
CD |
denotes |
1 |
T5425 |
731-732 |
SYM |
denotes |
: |
T5426 |
732-736 |
CD |
denotes |
1000 |
T5427 |
737-738 |
-LRB- |
denotes |
( |
T5429 |
738-740 |
NN |
denotes |
BD |
T5430 |
741-752 |
NNP |
denotes |
Biosciences |
T5428 |
753-761 |
NNP |
denotes |
Clontech |
T5431 |
761-762 |
-RRB- |
denotes |
) |
T5432 |
762-763 |
: |
denotes |
; |
T5433 |
764-767 |
NN |
denotes |
PDI |
T5434 |
768-769 |
-LRB- |
denotes |
( |
T5435 |
769-770 |
NN |
denotes |
H |
T5436 |
770-771 |
HYPH |
denotes |
- |
T5437 |
771-774 |
CD |
denotes |
160 |
T5438 |
774-775 |
-RRB- |
denotes |
) |
T5439 |
775-777 |
, |
denotes |
, |
T5440 |
777-778 |
CD |
denotes |
2 |
T5441 |
779-781 |
NN |
denotes |
μg |
T5442 |
781-782 |
SYM |
denotes |
/ |
T5443 |
782-784 |
NN |
denotes |
ml |
T5444 |
785-786 |
-LRB- |
denotes |
( |
T5446 |
786-791 |
NNP |
denotes |
Santa |
T5447 |
792-796 |
NNP |
denotes |
Cruz |
T5445 |
797-810 |
NNP |
denotes |
Biotechnology |
T5448 |
810-811 |
-RRB- |
denotes |
) |
T5449 |
811-812 |
: |
denotes |
; |
T5450 |
813-818 |
NN |
denotes |
GM130 |
T5451 |
818-820 |
, |
denotes |
, |
T5452 |
820-821 |
CD |
denotes |
1 |
T5453 |
822-824 |
NN |
denotes |
μg |
T5454 |
824-825 |
SYM |
denotes |
/ |
T5455 |
825-827 |
NN |
denotes |
ml |
T5456 |
828-829 |
-LRB- |
denotes |
( |
T5458 |
829-831 |
NN |
denotes |
BD |
T5459 |
832-844 |
NN |
denotes |
Transduction |
T5457 |
845-849 |
NNS |
denotes |
Labs |
T5460 |
849-850 |
-RRB- |
denotes |
) |
T5461 |
850-851 |
: |
denotes |
; |
T5462 |
852-853 |
NN |
denotes |
α |
T5464 |
853-854 |
HYPH |
denotes |
- |
T5463 |
854-861 |
NN |
denotes |
tubulin |
T5465 |
861-863 |
, |
denotes |
, |
T5466 |
863-864 |
CD |
denotes |
1 |
T5467 |
864-865 |
SYM |
denotes |
: |
T5468 |
865-871 |
CD |
denotes |
15,000 |
T5469 |
872-873 |
-LRB- |
denotes |
( |
T5470 |
873-878 |
NNP |
denotes |
Sigma |
T5471 |
878-879 |
-RRB- |
denotes |
) |
T5472 |
879-880 |
: |
denotes |
; |
T5473 |
881-886 |
NN |
denotes |
grp75 |
T5474 |
886-888 |
, |
denotes |
, |
T5475 |
888-889 |
CD |
denotes |
1 |
T5476 |
889-890 |
SYM |
denotes |
: |
T5477 |
890-893 |
CD |
denotes |
200 |
T5478 |
894-895 |
-LRB- |
denotes |
( |
T5479 |
895-904 |
NNP |
denotes |
Stressgen |
T5480 |
904-905 |
-RRB- |
denotes |
) |
T5481 |
905-906 |
: |
denotes |
; |
T5482 |
907-912 |
JJ |
denotes |
early |
T5484 |
913-921 |
NN |
denotes |
endosome |
T5483 |
922-929 |
NN |
denotes |
antigen |
T5485 |
929-930 |
HYPH |
denotes |
- |
T5486 |
930-931 |
CD |
denotes |
1 |
T5487 |
932-933 |
-LRB- |
denotes |
( |
T5488 |
933-937 |
NN |
denotes |
EEA1 |
T5489 |
937-938 |
-RRB- |
denotes |
) |
T5490 |
938-940 |
, |
denotes |
, |
T5491 |
940-941 |
CD |
denotes |
2 |
T5492 |
942-944 |
NN |
denotes |
μg |
T5493 |
944-945 |
SYM |
denotes |
/ |
T5494 |
945-947 |
NN |
denotes |
ml |
T5495 |
948-949 |
-LRB- |
denotes |
( |
T5497 |
949-954 |
NNP |
denotes |
Santa |
T5498 |
955-959 |
NNP |
denotes |
Cruz |
T5496 |
960-973 |
NNP |
denotes |
Biotechnology |
T5499 |
973-974 |
-RRB- |
denotes |
) |
T5500 |
974-975 |
: |
denotes |
; |
T5501 |
976-979 |
NN |
denotes |
rab |
T5502 |
980-981 |
CD |
denotes |
7 |
T5503 |
981-983 |
, |
denotes |
, |
T5504 |
983-984 |
CD |
denotes |
4 |
T5505 |
985-987 |
NN |
denotes |
μg |
T5506 |
987-988 |
SYM |
denotes |
/ |
T5507 |
988-990 |
NN |
denotes |
ml |
T5508 |
991-992 |
-LRB- |
denotes |
( |
T5510 |
992-997 |
NNP |
denotes |
Santa |
T5511 |
998-1002 |
NNP |
denotes |
Cruz |
T5509 |
1003-1016 |
NNP |
denotes |
Biotechnology |
T5512 |
1016-1017 |
-RRB- |
denotes |
) |
T5513 |
1017-1018 |
. |
denotes |
. |
T5514 |
1018-1191 |
sentence |
denotes |
All fluorescent secondary antibodies were obtained from Jackson ImmunoResearch Laboratories and HRP-conjugated secondary antibodies were obtained from Amersham Biosciences. |
T5515 |
1019-1022 |
DT |
denotes |
All |
T5517 |
1023-1034 |
JJ |
denotes |
fluorescent |
T5518 |
1035-1044 |
JJ |
denotes |
secondary |
T5516 |
1045-1055 |
NNS |
denotes |
antibodies |
T5520 |
1056-1060 |
VBD |
denotes |
were |
T5519 |
1061-1069 |
VBN |
denotes |
obtained |
T5521 |
1070-1074 |
IN |
denotes |
from |
T5522 |
1075-1082 |
NNP |
denotes |
Jackson |
T5524 |
1083-1097 |
NNP |
denotes |
ImmunoResearch |
T5523 |
1098-1110 |
NNP |
denotes |
Laboratories |
T5525 |
1111-1114 |
CC |
denotes |
and |
T5526 |
1115-1118 |
NN |
denotes |
HRP |
T5528 |
1118-1119 |
HYPH |
denotes |
- |
T5527 |
1119-1129 |
VBN |
denotes |
conjugated |
T5530 |
1130-1139 |
JJ |
denotes |
secondary |
T5529 |
1140-1150 |
NNS |
denotes |
antibodies |
T5532 |
1151-1155 |
VBD |
denotes |
were |
T5531 |
1156-1164 |
VBN |
denotes |
obtained |
T5533 |
1165-1169 |
IN |
denotes |
from |
T5534 |
1170-1178 |
NNP |
denotes |
Amersham |
T5535 |
1179-1190 |
NNP |
denotes |
Biosciences |
T5536 |
1190-1191 |
. |
denotes |
. |
T5537 |
1191-1361 |
sentence |
denotes |
Additional cell markers were as follows: VVL-biotin, 1:2000 (Vector Laboratories), 10,000 MW dextran-FITC, 1 mg/ml and Lysotracker Red DND-99, 500 nM (Molecular Probes). |
T5538 |
1192-1202 |
JJ |
denotes |
Additional |
T5540 |
1203-1207 |
NN |
denotes |
cell |
T5539 |
1208-1215 |
NNS |
denotes |
markers |
T5541 |
1216-1220 |
VBD |
denotes |
were |
T5542 |
1221-1223 |
IN |
denotes |
as |
T5543 |
1224-1231 |
VBZ |
denotes |
follows |
T5544 |
1231-1233 |
: |
denotes |
: |
T5545 |
1233-1236 |
NN |
denotes |
VVL |
T5547 |
1236-1237 |
HYPH |
denotes |
- |
T5546 |
1237-1243 |
NN |
denotes |
biotin |
T5548 |
1243-1245 |
, |
denotes |
, |
T5549 |
1245-1246 |
CD |
denotes |
1 |
T5550 |
1246-1247 |
SYM |
denotes |
: |
T5551 |
1247-1251 |
CD |
denotes |
2000 |
T5552 |
1252-1253 |
-LRB- |
denotes |
( |
T5554 |
1253-1259 |
NNP |
denotes |
Vector |
T5553 |
1260-1272 |
NNP |
denotes |
Laboratories |
T5555 |
1272-1273 |
-RRB- |
denotes |
) |
T5556 |
1273-1275 |
, |
denotes |
, |
T5557 |
1275-1281 |
CD |
denotes |
10,000 |
T5558 |
1282-1284 |
NN |
denotes |
MW |
T5560 |
1285-1292 |
NN |
denotes |
dextran |
T5561 |
1292-1293 |
HYPH |
denotes |
- |
T5559 |
1293-1297 |
NN |
denotes |
FITC |
T5562 |
1297-1299 |
, |
denotes |
, |
T5563 |
1299-1300 |
CD |
denotes |
1 |
T5564 |
1301-1303 |
NN |
denotes |
mg |
T5565 |
1303-1304 |
SYM |
denotes |
/ |
T5566 |
1304-1306 |
NN |
denotes |
ml |
T5567 |
1307-1310 |
CC |
denotes |
and |
T5568 |
1311-1322 |
NNP |
denotes |
Lysotracker |
T5570 |
1323-1326 |
NN |
denotes |
Red |
T5569 |
1327-1330 |
NN |
denotes |
DND |
T5571 |
1330-1331 |
HYPH |
denotes |
- |
T5572 |
1331-1333 |
CD |
denotes |
99 |
T5573 |
1333-1335 |
, |
denotes |
, |
T5574 |
1335-1338 |
CD |
denotes |
500 |
T5575 |
1339-1341 |
NN |
denotes |
nM |
T5576 |
1342-1343 |
-LRB- |
denotes |
( |
T5578 |
1343-1352 |
NNP |
denotes |
Molecular |
T5577 |
1353-1359 |
NNP |
denotes |
Probes |
T5579 |
1359-1360 |
-RRB- |
denotes |
) |
T5580 |
1360-1361 |
. |
denotes |
. |
R3000 |
T5236 |
T5237 |
nmod |
Antibody,reagents |
R3001 |
T5238 |
T5236 |
cc |
and,Antibody |
R3002 |
T5239 |
T5240 |
compound |
cell,staining |
R3003 |
T5240 |
T5236 |
conj |
staining,Antibody |
R3004 |
T5242 |
T5243 |
nmod |
Nestin,antibodies |
R3005 |
T5243 |
T5282 |
nsubjpass |
antibodies,obtained |
R3006 |
T5244 |
T5245 |
punct |
(,μg |
R3007 |
T5245 |
T5242 |
parataxis |
μg,Nestin |
R3008 |
T5246 |
T5247 |
compound |
clone,Rat |
R3009 |
T5247 |
T5245 |
dep |
Rat,μg |
R3010 |
T5248 |
T5247 |
nummod |
401,Rat |
R3011 |
T5249 |
T5245 |
punct |
", ",μg |
R3012 |
T5250 |
T5245 |
nummod |
2,μg |
R3013 |
T5251 |
T5252 |
punct |
/,ml |
R3014 |
T5252 |
T5245 |
prep |
ml,μg |
R3015 |
T5253 |
T5245 |
punct |
),μg |
R3016 |
T5254 |
T5242 |
punct |
", ",Nestin |
R3017 |
T5255 |
T5242 |
conj |
Lamp,Nestin |
R3018 |
T5256 |
T5255 |
nummod |
1,Lamp |
R3019 |
T5257 |
T5258 |
punct |
(,μg |
R3020 |
T5258 |
T5255 |
parataxis |
μg,Lamp |
R3021 |
T5259 |
T5260 |
compound |
clone,1D4B |
R3022 |
T5260 |
T5258 |
dep |
1D4B,μg |
R3023 |
T5261 |
T5258 |
punct |
", ",μg |
R3024 |
T5262 |
T5258 |
nummod |
6,μg |
R3025 |
T5263 |
T5264 |
punct |
/,ml |
R3026 |
T5264 |
T5258 |
prep |
ml,μg |
R3027 |
T5265 |
T5258 |
punct |
),μg |
R3028 |
T5266 |
T5255 |
punct |
", ",Lamp |
R3029 |
T5267 |
T5255 |
cc |
and,Lamp |
R3030 |
T5268 |
T5255 |
conj |
Lamp,Lamp |
R3031 |
T5269 |
T5268 |
nummod |
2,Lamp |
R3032 |
T5270 |
T5271 |
punct |
(,μg |
R3033 |
T5271 |
T5268 |
parataxis |
μg,Lamp |
R3034 |
T5272 |
T5273 |
compound |
clone,Abl |
R3035 |
T5273 |
T5271 |
dep |
Abl,μg |
R3036 |
T5274 |
T5273 |
punct |
-,Abl |
R3037 |
T5275 |
T5273 |
nummod |
93,Abl |
R3038 |
T5276 |
T5271 |
punct |
;,μg |
R3039 |
T5277 |
T5271 |
nummod |
6,μg |
R3040 |
T5278 |
T5279 |
punct |
/,ml |
R3041 |
T5279 |
T5271 |
prep |
ml,μg |
R3042 |
T5280 |
T5271 |
punct |
),μg |
R3043 |
T5281 |
T5243 |
amod |
monoclonal,antibodies |
R3044 |
T5283 |
T5282 |
auxpass |
were,obtained |
R3045 |
T5284 |
T5282 |
prep |
from,obtained |
R3046 |
T5285 |
T5286 |
det |
the,Bank |
R3047 |
T5286 |
T5284 |
pobj |
Bank,from |
R3048 |
T5287 |
T5286 |
compound |
Developmental,Bank |
R3049 |
T5288 |
T5286 |
compound |
Studies,Bank |
R3050 |
T5289 |
T5286 |
compound |
Hybridoma,Bank |
R3051 |
T5290 |
T5286 |
punct |
", ",Bank |
R3052 |
T5291 |
T5286 |
acl |
maintained,Bank |
R3053 |
T5292 |
T5291 |
agent |
by,maintained |
R3054 |
T5293 |
T5294 |
det |
The,University |
R3055 |
T5294 |
T5295 |
nmod |
University,Department |
R3056 |
T5295 |
T5292 |
pobj |
Department,by |
R3057 |
T5296 |
T5294 |
prep |
of,University |
R3058 |
T5297 |
T5296 |
pobj |
Iowa,of |
R3059 |
T5298 |
T5295 |
punct |
", ",Department |
R3060 |
T5299 |
T5295 |
prep |
of,Department |
R3061 |
T5300 |
T5301 |
compound |
Biological,Sciences |
R3062 |
T5301 |
T5299 |
pobj |
Sciences,of |
R3063 |
T5302 |
T5282 |
punct |
.,obtained |
R3064 |
T5304 |
T5305 |
nsubjpass |
Batp1,described |
R3065 |
T5306 |
T5307 |
punct |
(,μg |
R3066 |
T5307 |
T5304 |
parataxis |
μg,Batp1 |
R3067 |
T5308 |
T5307 |
nummod |
1,μg |
R3068 |
T5309 |
T5310 |
punct |
/,ml |
R3069 |
T5310 |
T5307 |
prep |
ml,μg |
R3070 |
T5311 |
T5307 |
punct |
),μg |
R3071 |
T5312 |
T5305 |
auxpass |
was,described |
R3072 |
T5313 |
T5305 |
advmod |
previously,described |
R3073 |
T5314 |
T5315 |
punct |
[,12 |
R3074 |
T5315 |
T5305 |
parataxis |
12,described |
R3075 |
T5316 |
T5315 |
punct |
],12 |
R3076 |
T5317 |
T5305 |
punct |
.,described |
R3077 |
T5319 |
T5320 |
amod |
Anti-subunit,c |
R3078 |
T5320 |
T5321 |
amod |
c,antibody |
R3079 |
T5321 |
T5322 |
nsubjpass |
antibody,provided |
R3080 |
T5323 |
T5324 |
punct |
(,μg |
R3081 |
T5324 |
T5321 |
parataxis |
μg,antibody |
R3082 |
T5325 |
T5324 |
nummod |
0.7,μg |
R3083 |
T5326 |
T5327 |
punct |
/,ml |
R3084 |
T5327 |
T5324 |
prep |
ml,μg |
R3085 |
T5328 |
T5324 |
punct |
),μg |
R3086 |
T5329 |
T5322 |
auxpass |
was,provided |
R3087 |
T5330 |
T5322 |
advmod |
kindly,provided |
R3088 |
T5331 |
T5322 |
agent |
by,provided |
R3089 |
T5332 |
T5333 |
compound |
Dr.,Kominami |
R3090 |
T5333 |
T5331 |
pobj |
Kominami,by |
R3091 |
T5334 |
T5335 |
punct |
(,University |
R3092 |
T5335 |
T5333 |
parataxis |
University,Kominami |
R3093 |
T5336 |
T5335 |
compound |
Juntendo,University |
R3094 |
T5337 |
T5335 |
punct |
", ",University |
R3095 |
T5338 |
T5335 |
npadvmod |
Tokyo,University |
R3096 |
T5339 |
T5335 |
punct |
", ",University |
R3097 |
T5340 |
T5335 |
npadvmod |
Japan,University |
R3098 |
T5341 |
T5335 |
punct |
),University |
R3099 |
T5342 |
T5322 |
punct |
.,provided |
R3100 |
T5344 |
T5345 |
amod |
Additional,antibodies |
R3101 |
T5345 |
T5346 |
nsubj |
antibodies,were |
R3102 |
T5347 |
T5348 |
mark |
as,follows |
R3103 |
T5348 |
T5346 |
advcl |
follows,were |
R3104 |
T5349 |
T5346 |
punct |
: ,were |
R3105 |
T5350 |
T5346 |
dep |
GFAP,were |
R3106 |
T5351 |
T5350 |
punct |
", ",GFAP |
R3107 |
T5352 |
T5350 |
npadvmod |
1,GFAP |
R3108 |
T5353 |
T5354 |
punct |
:,2000 |
R3109 |
T5354 |
T5352 |
prep |
2000,1 |
R3110 |
T5355 |
T5356 |
punct |
(,Corporation |
R3111 |
T5356 |
T5350 |
parataxis |
Corporation,GFAP |
R3112 |
T5357 |
T5356 |
compound |
DAKO,Corporation |
R3113 |
T5358 |
T5356 |
punct |
),Corporation |
R3114 |
T5359 |
T5350 |
punct |
;,GFAP |
R3115 |
T5360 |
T5350 |
appos |
calbindin,GFAP |
R3116 |
T5361 |
T5360 |
punct |
", ",calbindin |
R3117 |
T5362 |
T5360 |
npadvmod |
1,calbindin |
R3118 |
T5363 |
T5364 |
punct |
:,5000 |
R3119 |
T5364 |
T5362 |
prep |
5000,1 |
R3120 |
T5365 |
T5366 |
punct |
(,Sigma |
R3121 |
T5366 |
T5360 |
parataxis |
Sigma,calbindin |
R3122 |
T5367 |
T5366 |
punct |
),Sigma |
R3123 |
T5368 |
T5350 |
punct |
;,GFAP |
R3124 |
T5369 |
T5350 |
appos |
NeuN,GFAP |
R3125 |
T5370 |
T5369 |
punct |
", ",NeuN |
R3126 |
T5371 |
T5372 |
nummod |
10,μg |
R3127 |
T5372 |
T5369 |
npadvmod |
μg,NeuN |
R3128 |
T5373 |
T5374 |
punct |
/,ml |
R3129 |
T5374 |
T5372 |
prep |
ml,μg |
R3130 |
T5375 |
T5376 |
punct |
(,Chemicon |
R3131 |
T5376 |
T5369 |
parataxis |
Chemicon,NeuN |
R3132 |
T5377 |
T5376 |
punct |
),Chemicon |
R3133 |
T5378 |
T5350 |
punct |
;,GFAP |
R3134 |
T5379 |
T5380 |
compound |
SV40,antigen |
R3135 |
T5380 |
T5350 |
appos |
antigen,GFAP |
R3136 |
T5381 |
T5380 |
compound |
T,antigen |
R3137 |
T5382 |
T5380 |
punct |
(,antigen |
R3138 |
T5383 |
T5380 |
appos |
Pab,antigen |
R3139 |
T5384 |
T5383 |
nummod |
101,Pab |
R3140 |
T5385 |
T5380 |
punct |
),antigen |
R3141 |
T5386 |
T5380 |
punct |
", ",antigen |
R3142 |
T5387 |
T5388 |
nummod |
2,μg |
R3143 |
T5388 |
T5380 |
npadvmod |
μg,antigen |
R3144 |
T5389 |
T5390 |
punct |
/,ml |
R3145 |
T5390 |
T5388 |
prep |
ml,μg |
R3146 |
T5391 |
T5392 |
punct |
(,Biotechnology |
R3147 |
T5392 |
T5380 |
parataxis |
Biotechnology,antigen |
R3148 |
T5393 |
T5392 |
compound |
Santa,Biotechnology |
R3149 |
T5394 |
T5392 |
compound |
Cruz,Biotechnology |
R3150 |
T5395 |
T5392 |
punct |
),Biotechnology |
R3151 |
T5396 |
T5350 |
punct |
;,GFAP |
R3152 |
T5397 |
T5398 |
compound |
cathepsin,D |
R3153 |
T5398 |
T5350 |
appos |
D,GFAP |
R3154 |
T5399 |
T5398 |
punct |
(,D |
R3155 |
T5400 |
T5398 |
appos |
C,D |
R3156 |
T5401 |
T5400 |
punct |
-,C |
R3157 |
T5402 |
T5400 |
nummod |
20,C |
R3158 |
T5403 |
T5398 |
punct |
),D |
R3159 |
T5404 |
T5398 |
punct |
", ",D |
R3160 |
T5405 |
T5406 |
nummod |
2,μg |
R3161 |
T5406 |
T5398 |
npadvmod |
μg,D |
R3162 |
T5407 |
T5408 |
punct |
/,ml |
R3163 |
T5408 |
T5406 |
prep |
ml,μg |
R3164 |
T5409 |
T5410 |
punct |
(,Biotechnology |
R3165 |
T5410 |
T5398 |
parataxis |
Biotechnology,D |
R3166 |
T5411 |
T5410 |
compound |
Santa,Biotechnology |
R3167 |
T5412 |
T5410 |
compound |
Cruz,Biotechnology |
R3168 |
T5413 |
T5410 |
punct |
),Biotechnology |
R3169 |
T5414 |
T5350 |
punct |
;,GFAP |
R3170 |
T5415 |
T5416 |
compound |
cytochrome,subunit |
R3171 |
T5416 |
T5350 |
appos |
subunit,GFAP |
R3172 |
T5417 |
T5416 |
compound |
c,subunit |
R3173 |
T5418 |
T5416 |
compound |
oxidase,subunit |
R3174 |
T5419 |
T5416 |
nummod |
IV,subunit |
R3175 |
T5420 |
T5416 |
punct |
(,subunit |
R3176 |
T5421 |
T5416 |
appos |
cox4,subunit |
R3177 |
T5422 |
T5416 |
punct |
),subunit |
R3178 |
T5423 |
T5416 |
punct |
", ",subunit |
R3179 |
T5424 |
T5416 |
npadvmod |
1,subunit |
R3180 |
T5425 |
T5426 |
punct |
:,1000 |
R3181 |
T5426 |
T5424 |
prep |
1000,1 |
R3182 |
T5427 |
T5428 |
punct |
(,Clontech |
R3183 |
T5428 |
T5416 |
parataxis |
Clontech,subunit |
R3184 |
T5429 |
T5428 |
compound |
BD,Clontech |
R3185 |
T5430 |
T5428 |
compound |
Biosciences,Clontech |
R3186 |
T5431 |
T5428 |
punct |
),Clontech |
R3187 |
T5432 |
T5350 |
punct |
;,GFAP |
R3188 |
T5433 |
T5350 |
appos |
PDI,GFAP |
R3189 |
T5434 |
T5433 |
punct |
(,PDI |
R3190 |
T5435 |
T5433 |
appos |
H,PDI |
R3191 |
T5436 |
T5435 |
punct |
-,H |
R3192 |
T5437 |
T5435 |
nummod |
160,H |
R3193 |
T5438 |
T5433 |
punct |
),PDI |
R3194 |
T5439 |
T5433 |
punct |
", ",PDI |
R3195 |
T5440 |
T5441 |
nummod |
2,μg |
R3196 |
T5441 |
T5433 |
npadvmod |
μg,PDI |
R3197 |
T5442 |
T5443 |
punct |
/,ml |
R3198 |
T5443 |
T5441 |
prep |
ml,μg |
R3199 |
T5444 |
T5445 |
punct |
(,Biotechnology |
R3200 |
T5445 |
T5433 |
parataxis |
Biotechnology,PDI |
R3201 |
T5446 |
T5445 |
compound |
Santa,Biotechnology |
R3202 |
T5447 |
T5445 |
compound |
Cruz,Biotechnology |
R3203 |
T5448 |
T5445 |
punct |
),Biotechnology |
R3204 |
T5449 |
T5350 |
punct |
;,GFAP |
R3205 |
T5450 |
T5350 |
appos |
GM130,GFAP |
R3206 |
T5451 |
T5450 |
punct |
", ",GM130 |
R3207 |
T5452 |
T5453 |
nummod |
1,μg |
R3208 |
T5453 |
T5450 |
npadvmod |
μg,GM130 |
R3209 |
T5454 |
T5455 |
punct |
/,ml |
R3210 |
T5455 |
T5453 |
prep |
ml,μg |
R3211 |
T5456 |
T5457 |
punct |
(,Labs |
R3212 |
T5457 |
T5450 |
parataxis |
Labs,GM130 |
R3213 |
T5458 |
T5457 |
compound |
BD,Labs |
R3214 |
T5459 |
T5457 |
compound |
Transduction,Labs |
R3215 |
T5460 |
T5457 |
punct |
),Labs |
R3216 |
T5461 |
T5350 |
punct |
;,GFAP |
R3217 |
T5462 |
T5463 |
compound |
α,tubulin |
R3218 |
T5463 |
T5350 |
appos |
tubulin,GFAP |
R3219 |
T5464 |
T5463 |
punct |
-,tubulin |
R3220 |
T5465 |
T5463 |
punct |
", ",tubulin |
R3221 |
T5466 |
T5463 |
npadvmod |
1,tubulin |
R3222 |
T5467 |
T5468 |
punct |
:,"15,000" |
R3223 |
T5468 |
T5466 |
prep |
"15,000",1 |
R3224 |
T5469 |
T5470 |
punct |
(,Sigma |
R3225 |
T5470 |
T5463 |
parataxis |
Sigma,tubulin |
R3226 |
T5471 |
T5470 |
punct |
),Sigma |
R3227 |
T5472 |
T5350 |
punct |
;,GFAP |
R3228 |
T5473 |
T5350 |
appos |
grp75,GFAP |
R3229 |
T5474 |
T5473 |
punct |
", ",grp75 |
R3230 |
T5475 |
T5473 |
npadvmod |
1,grp75 |
R3231 |
T5476 |
T5477 |
punct |
:,200 |
R3232 |
T5477 |
T5475 |
prep |
200,1 |
R3233 |
T5478 |
T5479 |
punct |
(,Stressgen |
R3234 |
T5479 |
T5473 |
parataxis |
Stressgen,grp75 |
R3235 |
T5480 |
T5479 |
punct |
),Stressgen |
R3236 |
T5481 |
T5350 |
punct |
;,GFAP |
R3237 |
T5482 |
T5483 |
amod |
early,antigen |
R3238 |
T5483 |
T5350 |
appos |
antigen,GFAP |
R3239 |
T5484 |
T5483 |
compound |
endosome,antigen |
R3240 |
T5485 |
T5483 |
punct |
-,antigen |
R3241 |
T5486 |
T5483 |
nummod |
1,antigen |
R3242 |
T5487 |
T5483 |
punct |
(,antigen |
R3243 |
T5488 |
T5483 |
appos |
EEA1,antigen |
R3244 |
T5489 |
T5483 |
punct |
),antigen |
R3245 |
T5490 |
T5483 |
punct |
", ",antigen |
R3246 |
T5491 |
T5492 |
nummod |
2,μg |
R3247 |
T5492 |
T5483 |
npadvmod |
μg,antigen |
R3248 |
T5493 |
T5494 |
punct |
/,ml |
R3249 |
T5494 |
T5492 |
prep |
ml,μg |
R3250 |
T5495 |
T5496 |
punct |
(,Biotechnology |
R3251 |
T5496 |
T5483 |
parataxis |
Biotechnology,antigen |
R3252 |
T5497 |
T5496 |
compound |
Santa,Biotechnology |
R3253 |
T5498 |
T5496 |
compound |
Cruz,Biotechnology |
R3254 |
T5499 |
T5496 |
punct |
),Biotechnology |
R3255 |
T5500 |
T5350 |
punct |
;,GFAP |
R3256 |
T5501 |
T5350 |
appos |
rab,GFAP |
R3257 |
T5502 |
T5501 |
nummod |
7,rab |
R3258 |
T5503 |
T5501 |
punct |
", ",rab |
R3259 |
T5504 |
T5505 |
nummod |
4,μg |
R3260 |
T5505 |
T5501 |
npadvmod |
μg,rab |
R3261 |
T5506 |
T5507 |
punct |
/,ml |
R3262 |
T5507 |
T5505 |
prep |
ml,μg |
R3263 |
T5508 |
T5509 |
punct |
(,Biotechnology |
R3264 |
T5509 |
T5501 |
parataxis |
Biotechnology,rab |
R3265 |
T5510 |
T5509 |
compound |
Santa,Biotechnology |
R3266 |
T5511 |
T5509 |
compound |
Cruz,Biotechnology |
R3267 |
T5512 |
T5509 |
punct |
),Biotechnology |
R3268 |
T5513 |
T5346 |
punct |
.,were |
R3269 |
T5515 |
T5516 |
det |
All,antibodies |
R3270 |
T5516 |
T5519 |
nsubjpass |
antibodies,obtained |
R3271 |
T5517 |
T5516 |
amod |
fluorescent,antibodies |
R3272 |
T5518 |
T5516 |
amod |
secondary,antibodies |
R3273 |
T5520 |
T5519 |
auxpass |
were,obtained |
R3274 |
T5521 |
T5519 |
prep |
from,obtained |
R3275 |
T5522 |
T5523 |
compound |
Jackson,Laboratories |
R3276 |
T5523 |
T5521 |
pobj |
Laboratories,from |
R3277 |
T5524 |
T5523 |
compound |
ImmunoResearch,Laboratories |
R3278 |
T5525 |
T5519 |
cc |
and,obtained |
R3279 |
T5526 |
T5527 |
npadvmod |
HRP,conjugated |
R3280 |
T5527 |
T5529 |
amod |
conjugated,antibodies |
R3281 |
T5528 |
T5527 |
punct |
-,conjugated |
R3282 |
T5529 |
T5531 |
nsubjpass |
antibodies,obtained |
R3283 |
T5530 |
T5529 |
amod |
secondary,antibodies |
R3284 |
T5531 |
T5519 |
conj |
obtained,obtained |
R3285 |
T5532 |
T5531 |
auxpass |
were,obtained |
R3286 |
T5533 |
T5531 |
prep |
from,obtained |
R3287 |
T5534 |
T5535 |
compound |
Amersham,Biosciences |
R3288 |
T5535 |
T5533 |
pobj |
Biosciences,from |
R3289 |
T5536 |
T5531 |
punct |
.,obtained |
R3290 |
T5538 |
T5539 |
amod |
Additional,markers |
R3291 |
T5539 |
T5541 |
nsubj |
markers,were |
R3292 |
T5540 |
T5539 |
compound |
cell,markers |
R3293 |
T5542 |
T5543 |
mark |
as,follows |
R3294 |
T5543 |
T5541 |
advcl |
follows,were |
R3295 |
T5544 |
T5541 |
punct |
: ,were |
R3296 |
T5545 |
T5546 |
compound |
VVL,biotin |
R3297 |
T5546 |
T5541 |
dep |
biotin,were |
R3298 |
T5547 |
T5546 |
punct |
-,biotin |
R3299 |
T5548 |
T5546 |
punct |
", ",biotin |
R3300 |
T5549 |
T5546 |
npadvmod |
1,biotin |
R3301 |
T5550 |
T5551 |
punct |
:,2000 |
R3302 |
T5551 |
T5549 |
prep |
2000,1 |
R3303 |
T5552 |
T5553 |
punct |
(,Laboratories |
R3304 |
T5553 |
T5546 |
parataxis |
Laboratories,biotin |
R3305 |
T5554 |
T5553 |
compound |
Vector,Laboratories |
R3306 |
T5555 |
T5553 |
punct |
),Laboratories |
R3307 |
T5556 |
T5546 |
punct |
", ",biotin |
R3308 |
T5557 |
T5558 |
nummod |
"10,000",MW |
R3309 |
T5558 |
T5559 |
compound |
MW,FITC |
R3310 |
T5559 |
T5546 |
conj |
FITC,biotin |
R3311 |
T5560 |
T5559 |
compound |
dextran,FITC |
R3312 |
T5561 |
T5559 |
punct |
-,FITC |
R3313 |
T5562 |
T5559 |
punct |
", ",FITC |
R3314 |
T5563 |
T5564 |
nummod |
1,mg |
R3315 |
T5564 |
T5559 |
npadvmod |
mg,FITC |
R3316 |
T5565 |
T5566 |
punct |
/,ml |
R3317 |
T5566 |
T5564 |
prep |
ml,mg |
R3318 |
T5567 |
T5559 |
cc |
and,FITC |
R3319 |
T5568 |
T5569 |
compound |
Lysotracker,DND |
R3320 |
T5569 |
T5559 |
conj |
DND,FITC |
R3321 |
T5570 |
T5569 |
compound |
Red,DND |
R3322 |
T5571 |
T5569 |
punct |
-,DND |
R3323 |
T5572 |
T5569 |
nummod |
99,DND |
R3324 |
T5573 |
T5569 |
punct |
", ",DND |
R3325 |
T5574 |
T5575 |
nummod |
500,nM |
R3326 |
T5575 |
T5569 |
npadvmod |
nM,DND |
R3327 |
T5576 |
T5577 |
punct |
(,Probes |
R3328 |
T5577 |
T5569 |
parataxis |
Probes,DND |
R3329 |
T5578 |
T5577 |
compound |
Molecular,Probes |
R3330 |
T5579 |
T5577 |
punct |
),Probes |
R3331 |
T5580 |
T5541 |
punct |
.,were |