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PMC:539297 / 11990-13614 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T2544 0-2 PRP denotes We
T2546 3-7 RB denotes next
T2545 8-20 VBD denotes investigated
T2547 21-24 DT denotes the
T2548 25-30 NN denotes basis
T2549 31-34 IN denotes for
T2550 35-42 NN denotes subunit
T2551 43-44 NN denotes c
T2552 45-57 NN denotes accumulation
T2553 57-59 , denotes ,
T2554 59-66 VBG denotes testing
T2555 67-70 DT denotes the
T2556 71-81 NN denotes hypothesis
T2557 82-86 IN denotes that
T2559 87-96 NN denotes cathepsin
T2560 97-98 NN denotes D
T2558 99-101 VBZ denotes is
T2561 102-110 JJ denotes abnormal
T2562 111-116 IN denotes since
T2564 117-119 PRP denotes it
T2563 120-122 VBZ denotes is
T2565 123-131 JJ denotes required
T2566 132-135 IN denotes for
T2567 136-139 NN denotes ATP
T2569 140-148 NN denotes synthase
T2570 149-156 NN denotes subunit
T2571 157-158 NN denotes c
T2568 159-170 NN denotes degradation
T2572 171-173 IN denotes in
T2573 174-177 DT denotes the
T2574 178-186 NN denotes lysosome
T2575 187-188 -LRB- denotes [
T2576 188-190 CD denotes 13
T2577 190-191 -RRB- denotes ]
T2578 191-192 . denotes .
T2579 192-394 sentence denotes We first tested cathepsin D transport and processing in homozygous CbCln3Δex7/8 cells and Cln3Δex7/8 mice using anti-cathepsin D antibody that recognizes unprocessed and processed cathepsin D isoforms.
T2580 193-195 PRP denotes We
T2582 196-201 RB denotes first
T2581 202-208 VBD denotes tested
T2583 209-218 NN denotes cathepsin
T2584 219-220 NN denotes D
T2585 221-230 NN denotes transport
T2586 231-234 CC denotes and
T2587 235-245 NN denotes processing
T2588 246-248 IN denotes in
T2589 249-259 JJ denotes homozygous
T2591 260-270 NN denotes CbCln3Δex7
T2592 270-271 HYPH denotes /
T2593 271-272 CD denotes 8
T2590 273-278 NNS denotes cells
T2594 279-282 CC denotes and
T2595 283-291 NN denotes Cln3Δex7
T2597 291-292 HYPH denotes /
T2598 292-293 CD denotes 8
T2596 294-298 NNS denotes mice
T2599 299-304 VBG denotes using
T2600 305-319 JJ denotes anti-cathepsin
T2601 320-321 NN denotes D
T2602 322-330 NN denotes antibody
T2603 331-335 WDT denotes that
T2604 336-346 VBZ denotes recognizes
T2605 347-358 JJ denotes unprocessed
T2607 359-362 CC denotes and
T2608 363-372 VBN denotes processed
T2609 373-382 NN denotes cathepsin
T2610 383-384 NN denotes D
T2606 385-393 NNS denotes isoforms
T2611 393-394 . denotes .
T2612 394-643 sentence denotes Immunostaining of wild-type and homozygous CbCln3Δex7/8 cells revealed a perinuclear and punctate vesicular cathepsin D distribution, consistent with its transport and processing through the secretory pathway and delivery to the lysosome (Fig. 5a).
T2613 395-409 NN denotes Immunostaining
T2615 410-412 IN denotes of
T2616 413-417 JJ denotes wild
T2618 417-418 HYPH denotes -
T2617 418-422 NN denotes type
T2620 423-426 CC denotes and
T2621 427-437 JJ denotes homozygous
T2622 438-448 NN denotes CbCln3Δex7
T2623 448-449 HYPH denotes /
T2624 449-450 CD denotes 8
T2619 451-456 NNS denotes cells
T2614 457-465 VBD denotes revealed
T2625 466-467 DT denotes a
T2627 468-479 JJ denotes perinuclear
T2628 480-483 CC denotes and
T2629 484-492 JJ denotes punctate
T2630 493-502 JJ denotes vesicular
T2631 503-512 NN denotes cathepsin
T2632 513-514 NN denotes D
T2626 515-527 NN denotes distribution
T2633 527-529 , denotes ,
T2634 529-539 JJ denotes consistent
T2635 540-544 IN denotes with
T2636 545-548 PRP$ denotes its
T2637 549-558 NN denotes transport
T2638 559-562 CC denotes and
T2639 563-573 NN denotes processing
T2640 574-581 IN denotes through
T2641 582-585 DT denotes the
T2643 586-595 JJ denotes secretory
T2642 596-603 NN denotes pathway
T2644 604-607 CC denotes and
T2645 608-616 NN denotes delivery
T2646 617-619 IN denotes to
T2647 620-623 DT denotes the
T2648 624-632 NN denotes lysosome
T2649 633-634 -LRB- denotes (
T2651 634-638 NN denotes Fig.
T2650 639-641 NN denotes 5a
T2652 641-642 -RRB- denotes )
T2653 642-643 . denotes .
T2654 643-786 sentence denotes However, in homozygous CbCln3Δex7/8 cells, cathepsin D distribution was less vesicular and more perinuclear-clustered than in wild-type cells.
T2655 644-651 RB denotes However
T2657 651-653 , denotes ,
T2658 653-655 IN denotes in
T2659 656-666 JJ denotes homozygous
T2661 667-677 NN denotes CbCln3Δex7
T2662 677-678 HYPH denotes /
T2663 678-679 CD denotes 8
T2660 680-685 NNS denotes cells
T2664 685-687 , denotes ,
T2665 687-696 NN denotes cathepsin
T2666 697-698 NN denotes D
T2667 699-711 NN denotes distribution
T2656 712-715 VBD denotes was
T2668 716-720 RBR denotes less
T2669 721-730 JJ denotes vesicular
T2670 731-734 CC denotes and
T2671 735-739 RBR denotes more
T2673 740-751 JJ denotes perinuclear
T2674 751-752 HYPH denotes -
T2672 752-761 VBN denotes clustered
T2675 762-766 IN denotes than
T2676 767-769 IN denotes in
T2677 770-774 JJ denotes wild
T2679 774-775 HYPH denotes -
T2678 775-779 NN denotes type
T2680 780-785 NNS denotes cells
T2681 785-786 . denotes .
T2682 786-932 sentence denotes Immunoblots of homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts also showed altered relative levels of cathepsin D isoforms (Fig. 5b).
T2683 787-798 NNS denotes Immunoblots
T2685 799-801 IN denotes of
T2686 802-812 JJ denotes homozygous
T2688 813-823 NN denotes CbCln3Δex7
T2690 823-824 HYPH denotes /
T2691 824-825 CD denotes 8
T2689 826-830 NN denotes cell
T2692 831-834 CC denotes and
T2693 835-843 NN denotes Cln3Δex7
T2695 843-844 HYPH denotes /
T2696 844-845 CD denotes 8
T2694 846-852 NN denotes tissue
T2687 853-861 NNS denotes extracts
T2697 862-866 RB denotes also
T2684 867-873 VBD denotes showed
T2698 874-881 VBN denotes altered
T2700 882-890 JJ denotes relative
T2699 891-897 NNS denotes levels
T2701 898-900 IN denotes of
T2702 901-910 NN denotes cathepsin
T2703 911-912 NN denotes D
T2704 913-921 NNS denotes isoforms
T2705 922-923 -LRB- denotes (
T2707 923-927 NN denotes Fig.
T2706 928-930 NN denotes 5b
T2708 930-931 -RRB- denotes )
T2709 931-932 . denotes .
T2710 932-1166 sentence denotes Cathepsin D isoforms, identified by relative molecular weights, represent the ~45 kDa precursor, the ~43 kDa intermediate single chain form of the enzyme, and the 31 kDa heavy chain of the double-chain form of the mature enzyme [14].
T2711 933-942 NN denotes Cathepsin
T2712 943-944 NN denotes D
T2713 945-953 NNS denotes isoforms
T2715 953-955 , denotes ,
T2716 955-965 VBN denotes identified
T2717 966-968 IN denotes by
T2718 969-977 JJ denotes relative
T2720 978-987 JJ denotes molecular
T2719 988-995 NNS denotes weights
T2721 995-997 , denotes ,
T2714 997-1006 VBP denotes represent
T2722 1007-1010 DT denotes the
T2724 1011-1012 SYM denotes ~
T2725 1012-1014 CD denotes 45
T2726 1015-1018 NN denotes kDa
T2723 1019-1028 NN denotes precursor
T2727 1028-1030 , denotes ,
T2728 1030-1033 DT denotes the
T2730 1034-1035 SYM denotes ~
T2731 1035-1037 CD denotes 43
T2732 1038-1041 NN denotes kDa
T2733 1042-1054 JJ denotes intermediate
T2734 1055-1061 JJ denotes single
T2735 1062-1067 NN denotes chain
T2729 1068-1072 NN denotes form
T2736 1073-1075 IN denotes of
T2737 1076-1079 DT denotes the
T2738 1080-1086 NN denotes enzyme
T2739 1086-1088 , denotes ,
T2740 1088-1091 CC denotes and
T2741 1092-1095 DT denotes the
T2743 1096-1098 CD denotes 31
T2744 1099-1102 NN denotes kDa
T2745 1103-1108 JJ denotes heavy
T2742 1109-1114 NN denotes chain
T2746 1115-1117 IN denotes of
T2747 1118-1121 DT denotes the
T2749 1122-1128 JJ denotes double
T2751 1128-1129 HYPH denotes -
T2750 1129-1134 NN denotes chain
T2748 1135-1139 NN denotes form
T2752 1140-1142 IN denotes of
T2753 1143-1146 DT denotes the
T2755 1147-1153 JJ denotes mature
T2754 1154-1160 NN denotes enzyme
T2756 1161-1162 -LRB- denotes [
T2757 1162-1164 CD denotes 14
T2758 1164-1165 -RRB- denotes ]
T2759 1165-1166 . denotes .
T2760 1166-1350 sentence denotes In homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts, the precursor and heavy chains were reduced, and the single chain was slightly elevated compared to wild-type extracts.
T2761 1167-1169 IN denotes In
T2763 1170-1180 JJ denotes homozygous
T2765 1181-1191 NN denotes CbCln3Δex7
T2767 1191-1192 HYPH denotes /
T2768 1192-1193 CD denotes 8
T2766 1194-1198 NN denotes cell
T2769 1199-1202 CC denotes and
T2770 1203-1211 NN denotes Cln3Δex7
T2772 1211-1212 HYPH denotes /
T2773 1212-1213 CD denotes 8
T2771 1214-1220 NN denotes tissue
T2764 1221-1229 NNS denotes extracts
T2774 1229-1231 , denotes ,
T2775 1231-1234 DT denotes the
T2776 1235-1244 NN denotes precursor
T2777 1245-1248 CC denotes and
T2778 1249-1254 JJ denotes heavy
T2779 1255-1261 NNS denotes chains
T2780 1262-1266 VBD denotes were
T2762 1267-1274 VBN denotes reduced
T2781 1274-1276 , denotes ,
T2782 1276-1279 CC denotes and
T2783 1280-1283 DT denotes the
T2785 1284-1290 JJ denotes single
T2784 1291-1296 NN denotes chain
T2786 1297-1300 VBD denotes was
T2787 1301-1309 RB denotes slightly
T2788 1310-1318 JJ denotes elevated
T2789 1319-1327 VBN denotes compared
T2790 1328-1330 IN denotes to
T2791 1331-1335 JJ denotes wild
T2793 1335-1336 HYPH denotes -
T2792 1336-1340 NN denotes type
T2794 1341-1349 NNS denotes extracts
T2795 1349-1350 . denotes .
T2796 1350-1469 sentence denotes The cellular growth media did not contain altered levels of cathepsin D, indicating enzyme secretion was not affected.
T2797 1351-1354 DT denotes The
T2799 1355-1363 JJ denotes cellular
T2800 1364-1370 NN denotes growth
T2798 1371-1376 NNS denotes media
T2802 1377-1380 VBD denotes did
T2803 1381-1384 RB denotes not
T2801 1385-1392 VB denotes contain
T2804 1393-1400 VBN denotes altered
T2805 1401-1407 NNS denotes levels
T2806 1408-1410 IN denotes of
T2807 1411-1420 NN denotes cathepsin
T2808 1421-1422 NN denotes D
T2809 1422-1424 , denotes ,
T2810 1424-1434 VBG denotes indicating
T2811 1435-1441 NN denotes enzyme
T2812 1442-1451 NN denotes secretion
T2814 1452-1455 VBD denotes was
T2815 1456-1459 RB denotes not
T2813 1460-1468 VBN denotes affected
T2816 1468-1469 . denotes .
T2817 1469-1624 sentence denotes Heterozygous Cln3Δex7/8 mice and CbCln3Δex7/8 cells were indistinguishable from wild-type, as expected for a recessive disease phenotype (data not shown).
T2818 1470-1482 JJ denotes Heterozygous
T2820 1483-1491 NN denotes Cln3Δex7
T2821 1491-1492 HYPH denotes /
T2822 1492-1493 CD denotes 8
T2819 1494-1498 NNS denotes mice
T2824 1499-1502 CC denotes and
T2825 1503-1513 NN denotes CbCln3Δex7
T2827 1513-1514 HYPH denotes /
T2828 1514-1515 CD denotes 8
T2826 1516-1521 NNS denotes cells
T2823 1522-1526 VBD denotes were
T2829 1527-1544 JJ denotes indistinguishable
T2830 1545-1549 IN denotes from
T2831 1550-1554 JJ denotes wild
T2833 1554-1555 HYPH denotes -
T2832 1555-1559 NN denotes type
T2834 1559-1561 , denotes ,
T2835 1561-1563 IN denotes as
T2836 1564-1572 VBN denotes expected
T2837 1573-1576 IN denotes for
T2838 1577-1578 DT denotes a
T2840 1579-1588 JJ denotes recessive
T2841 1589-1596 NN denotes disease
T2839 1597-1606 NN denotes phenotype
T2842 1607-1608 -LRB- denotes (
T2844 1608-1612 NNS denotes data
T2845 1613-1616 RB denotes not
T2843 1617-1622 VBN denotes shown
T2846 1622-1623 -RRB- denotes )
T2847 1623-1624 . denotes .
R1391 T2544 T2545 nsubj We,investigated
R1392 T2546 T2545 advmod next,investigated
R1393 T2547 T2548 det the,basis
R1394 T2548 T2545 dobj basis,investigated
R1395 T2549 T2548 prep for,basis
R1396 T2550 T2551 compound subunit,c
R1397 T2551 T2552 compound c,accumulation
R1398 T2552 T2549 pobj accumulation,for
R1399 T2553 T2545 punct ", ",investigated
R1400 T2554 T2545 advcl testing,investigated
R1401 T2555 T2556 det the,hypothesis
R1402 T2556 T2554 dobj hypothesis,testing
R1403 T2557 T2558 mark that,is
R1404 T2558 T2556 acl is,hypothesis
R1405 T2559 T2560 compound cathepsin,D
R1406 T2560 T2558 nsubj D,is
R1407 T2561 T2558 acomp abnormal,is
R1408 T2562 T2563 mark since,is
R1409 T2563 T2558 advcl is,is
R1410 T2564 T2563 nsubj it,is
R1411 T2565 T2563 acomp required,is
R1412 T2566 T2565 prep for,required
R1413 T2567 T2568 compound ATP,degradation
R1414 T2568 T2566 pobj degradation,for
R1415 T2569 T2568 compound synthase,degradation
R1416 T2570 T2571 compound subunit,c
R1417 T2571 T2568 compound c,degradation
R1418 T2572 T2568 prep in,degradation
R1419 T2573 T2574 det the,lysosome
R1420 T2574 T2572 pobj lysosome,in
R1421 T2575 T2576 punct [,13
R1422 T2576 T2554 parataxis 13,testing
R1423 T2577 T2576 punct ],13
R1424 T2578 T2545 punct .,investigated
R1425 T2580 T2581 nsubj We,tested
R1426 T2582 T2581 advmod first,tested
R1427 T2583 T2584 compound cathepsin,D
R1428 T2584 T2581 dobj D,tested
R1429 T2585 T2584 appos transport,D
R1430 T2586 T2585 cc and,transport
R1431 T2587 T2585 conj processing,transport
R1432 T2588 T2581 prep in,tested
R1433 T2589 T2590 amod homozygous,cells
R1434 T2590 T2588 pobj cells,in
R1435 T2591 T2590 nmod CbCln3Δex7,cells
R1436 T2592 T2591 punct /,CbCln3Δex7
R1437 T2593 T2591 nummod 8,CbCln3Δex7
R1438 T2594 T2590 cc and,cells
R1439 T2595 T2596 nmod Cln3Δex7,mice
R1440 T2596 T2590 conj mice,cells
R1441 T2597 T2595 punct /,Cln3Δex7
R1442 T2598 T2595 nummod 8,Cln3Δex7
R1443 T2599 T2581 advcl using,tested
R1444 T2600 T2601 amod anti-cathepsin,D
R1445 T2601 T2602 amod D,antibody
R1446 T2602 T2599 dobj antibody,using
R1447 T2603 T2604 dep that,recognizes
R1448 T2604 T2602 relcl recognizes,antibody
R1449 T2605 T2606 amod unprocessed,isoforms
R1450 T2606 T2604 dobj isoforms,recognizes
R1451 T2607 T2605 cc and,unprocessed
R1452 T2608 T2605 conj processed,unprocessed
R1453 T2609 T2610 compound cathepsin,D
R1454 T2610 T2606 compound D,isoforms
R1455 T2611 T2581 punct .,tested
R1456 T2613 T2614 nsubj Immunostaining,revealed
R1457 T2615 T2613 prep of,Immunostaining
R1458 T2616 T2617 amod wild,type
R1459 T2617 T2619 nmod type,cells
R1460 T2618 T2617 punct -,type
R1461 T2619 T2615 pobj cells,of
R1462 T2620 T2617 cc and,type
R1463 T2621 T2617 conj homozygous,type
R1464 T2622 T2619 nmod CbCln3Δex7,cells
R1465 T2623 T2622 punct /,CbCln3Δex7
R1466 T2624 T2622 nummod 8,CbCln3Δex7
R1467 T2625 T2626 det a,distribution
R1468 T2626 T2614 dobj distribution,revealed
R1469 T2627 T2626 amod perinuclear,distribution
R1470 T2628 T2627 cc and,perinuclear
R1471 T2629 T2627 conj punctate,perinuclear
R1472 T2630 T2626 amod vesicular,distribution
R1473 T2631 T2632 compound cathepsin,D
R1474 T2632 T2626 compound D,distribution
R1475 T2633 T2614 punct ", ",revealed
R1476 T2634 T2614 advcl consistent,revealed
R1477 T2635 T2634 prep with,consistent
R1478 T2636 T2637 poss its,transport
R1479 T2637 T2635 pobj transport,with
R1480 T2638 T2637 cc and,transport
R1481 T2639 T2637 conj processing,transport
R1482 T2640 T2637 prep through,transport
R1483 T2641 T2642 det the,pathway
R1484 T2642 T2640 pobj pathway,through
R1485 T2643 T2642 amod secretory,pathway
R1486 T2644 T2637 cc and,transport
R1487 T2645 T2637 conj delivery,transport
R1488 T2646 T2645 prep to,delivery
R1489 T2647 T2648 det the,lysosome
R1490 T2648 T2646 pobj lysosome,to
R1491 T2649 T2650 punct (,5a
R1492 T2650 T2614 parataxis 5a,revealed
R1493 T2651 T2650 compound Fig.,5a
R1494 T2652 T2650 punct ),5a
R1495 T2653 T2614 punct .,revealed
R1496 T2655 T2656 advmod However,was
R1497 T2657 T2656 punct ", ",was
R1498 T2658 T2656 prep in,was
R1499 T2659 T2660 amod homozygous,cells
R1500 T2660 T2658 pobj cells,in
R1501 T2661 T2660 nmod CbCln3Δex7,cells
R1502 T2662 T2661 punct /,CbCln3Δex7
R1503 T2663 T2661 nummod 8,CbCln3Δex7
R1504 T2664 T2656 punct ", ",was
R1505 T2665 T2666 compound cathepsin,D
R1506 T2666 T2667 compound D,distribution
R1507 T2667 T2656 nsubj distribution,was
R1508 T2668 T2669 advmod less,vesicular
R1509 T2669 T2656 acomp vesicular,was
R1510 T2670 T2669 cc and,vesicular
R1511 T2671 T2672 advmod more,clustered
R1512 T2672 T2669 conj clustered,vesicular
R1513 T2673 T2672 amod perinuclear,clustered
R1514 T2674 T2672 punct -,clustered
R1515 T2675 T2669 prep than,vesicular
R1516 T2676 T2675 prep in,than
R1517 T2677 T2678 amod wild,type
R1518 T2678 T2680 compound type,cells
R1519 T2679 T2678 punct -,type
R1520 T2680 T2676 pobj cells,in
R1521 T2681 T2656 punct .,was
R1522 T2683 T2684 nsubj Immunoblots,showed
R1523 T2685 T2683 prep of,Immunoblots
R1524 T2686 T2687 amod homozygous,extracts
R1525 T2687 T2685 pobj extracts,of
R1526 T2688 T2689 nmod CbCln3Δex7,cell
R1527 T2689 T2687 nmod cell,extracts
R1528 T2690 T2688 punct /,CbCln3Δex7
R1529 T2691 T2688 nummod 8,CbCln3Δex7
R1530 T2692 T2689 cc and,cell
R1531 T2693 T2694 nmod Cln3Δex7,tissue
R1532 T2694 T2689 conj tissue,cell
R1533 T2695 T2693 punct /,Cln3Δex7
R1534 T2696 T2693 nummod 8,Cln3Δex7
R1535 T2697 T2684 advmod also,showed
R1536 T2698 T2699 amod altered,levels
R1537 T2699 T2684 dobj levels,showed
R1538 T2700 T2699 amod relative,levels
R1539 T2701 T2699 prep of,levels
R1540 T2702 T2703 compound cathepsin,D
R1541 T2703 T2704 compound D,isoforms
R1542 T2704 T2701 pobj isoforms,of
R1543 T2705 T2706 punct (,5b
R1544 T2706 T2684 parataxis 5b,showed
R1545 T2707 T2706 compound Fig.,5b
R1546 T2708 T2706 punct ),5b
R1547 T2709 T2684 punct .,showed
R1548 T2711 T2712 compound Cathepsin,D
R1549 T2712 T2713 compound D,isoforms
R1550 T2713 T2714 nsubj isoforms,represent
R1551 T2715 T2713 punct ", ",isoforms
R1552 T2716 T2713 acl identified,isoforms
R1553 T2717 T2716 agent by,identified
R1554 T2718 T2719 amod relative,weights
R1555 T2719 T2717 pobj weights,by
R1556 T2720 T2719 amod molecular,weights
R1557 T2721 T2714 punct ", ",represent
R1558 T2722 T2723 det the,precursor
R1559 T2723 T2714 dobj precursor,represent
R1560 T2724 T2725 punct ~,45
R1561 T2725 T2726 nummod 45,kDa
R1562 T2726 T2723 compound kDa,precursor
R1563 T2727 T2723 punct ", ",precursor
R1564 T2728 T2729 det the,form
R1565 T2729 T2723 conj form,precursor
R1566 T2730 T2731 punct ~,43
R1567 T2731 T2732 nummod 43,kDa
R1568 T2732 T2729 nmod kDa,form
R1569 T2733 T2729 amod intermediate,form
R1570 T2734 T2735 amod single,chain
R1571 T2735 T2729 compound chain,form
R1572 T2736 T2729 prep of,form
R1573 T2737 T2738 det the,enzyme
R1574 T2738 T2736 pobj enzyme,of
R1575 T2739 T2729 punct ", ",form
R1576 T2740 T2729 cc and,form
R1577 T2741 T2742 det the,chain
R1578 T2742 T2729 conj chain,form
R1579 T2743 T2744 nummod 31,kDa
R1580 T2744 T2742 nmod kDa,chain
R1581 T2745 T2742 amod heavy,chain
R1582 T2746 T2742 prep of,chain
R1583 T2747 T2748 det the,form
R1584 T2748 T2746 pobj form,of
R1585 T2749 T2750 amod double,chain
R1586 T2750 T2748 compound chain,form
R1587 T2751 T2750 punct -,chain
R1588 T2752 T2748 prep of,form
R1589 T2753 T2754 det the,enzyme
R1590 T2754 T2752 pobj enzyme,of
R1591 T2755 T2754 amod mature,enzyme
R1592 T2756 T2757 punct [,14
R1593 T2757 T2714 parataxis 14,represent
R1594 T2758 T2757 punct ],14
R1595 T2759 T2714 punct .,represent
R1596 T2761 T2762 prep In,reduced
R1597 T2763 T2764 amod homozygous,extracts
R1598 T2764 T2761 pobj extracts,In
R1599 T2765 T2766 nmod CbCln3Δex7,cell
R1600 T2766 T2764 nmod cell,extracts
R1601 T2767 T2765 punct /,CbCln3Δex7
R1602 T2768 T2765 nummod 8,CbCln3Δex7
R1603 T2769 T2766 cc and,cell
R1604 T2770 T2771 nmod Cln3Δex7,tissue
R1605 T2771 T2766 conj tissue,cell
R1606 T2772 T2770 punct /,Cln3Δex7
R1607 T2773 T2770 nummod 8,Cln3Δex7
R1608 T2774 T2762 punct ", ",reduced
R1609 T2775 T2776 det the,precursor
R1610 T2776 T2762 nsubjpass precursor,reduced
R1611 T2777 T2776 cc and,precursor
R1612 T2778 T2779 amod heavy,chains
R1613 T2779 T2776 conj chains,precursor
R1614 T2780 T2762 auxpass were,reduced
R1615 T2781 T2762 punct ", ",reduced
R1616 T2782 T2762 cc and,reduced
R1617 T2783 T2784 det the,chain
R1618 T2784 T2786 nsubj chain,was
R1619 T2785 T2784 amod single,chain
R1620 T2786 T2762 conj was,reduced
R1621 T2787 T2788 advmod slightly,elevated
R1622 T2788 T2786 acomp elevated,was
R1623 T2789 T2786 prep compared,was
R1624 T2790 T2789 prep to,compared
R1625 T2791 T2792 amod wild,type
R1626 T2792 T2794 compound type,extracts
R1627 T2793 T2792 punct -,type
R1628 T2794 T2790 pobj extracts,to
R1629 T2795 T2786 punct .,was
R1630 T2797 T2798 det The,media
R1631 T2798 T2801 nsubj media,contain
R1632 T2799 T2800 amod cellular,growth
R1633 T2800 T2798 compound growth,media
R1634 T2802 T2801 aux did,contain
R1635 T2803 T2801 neg not,contain
R1636 T2804 T2805 amod altered,levels
R1637 T2805 T2801 dobj levels,contain
R1638 T2806 T2805 prep of,levels
R1639 T2807 T2808 compound cathepsin,D
R1640 T2808 T2806 pobj D,of
R1641 T2809 T2801 punct ", ",contain
R1642 T2810 T2801 advcl indicating,contain
R1643 T2811 T2812 compound enzyme,secretion
R1644 T2812 T2813 nsubjpass secretion,affected
R1645 T2813 T2810 advcl affected,indicating
R1646 T2814 T2813 auxpass was,affected
R1647 T2815 T2813 neg not,affected
R1648 T2816 T2801 punct .,contain
R1649 T2818 T2819 amod Heterozygous,mice
R1650 T2819 T2823 nsubj mice,were
R1651 T2820 T2819 nmod Cln3Δex7,mice
R1652 T2821 T2820 punct /,Cln3Δex7
R1653 T2822 T2820 nummod 8,Cln3Δex7
R1654 T2824 T2819 cc and,mice
R1655 T2825 T2826 nmod CbCln3Δex7,cells
R1656 T2826 T2819 conj cells,mice
R1657 T2827 T2825 punct /,CbCln3Δex7
R1658 T2828 T2825 nummod 8,CbCln3Δex7
R1659 T2829 T2823 acomp indistinguishable,were
R1660 T2830 T2829 prep from,indistinguishable
R1661 T2831 T2832 amod wild,type
R1662 T2832 T2830 pobj type,from
R1663 T2833 T2832 punct -,type
R1664 T2834 T2823 punct ", ",were
R1665 T2835 T2836 mark as,expected
R1666 T2836 T2823 advcl expected,were
R1667 T2837 T2836 prep for,expected
R1668 T2838 T2839 det a,phenotype
R1669 T2839 T2837 pobj phenotype,for
R1670 T2840 T2839 amod recessive,phenotype
R1671 T2841 T2839 compound disease,phenotype
R1672 T2842 T2843 punct (,shown
R1673 T2843 T2836 parataxis shown,expected
R1674 T2844 T2843 nsubj data,shown
R1675 T2845 T2843 neg not,shown
R1676 T2846 T2843 punct ),shown
R1677 T2847 T2823 punct .,were