PMC:539297 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T170	0-8	NN	denotes	Membrane
T172	0-151	sentence	denotes	Membrane trafficking and mitochondrial abnormalities precede subunit c deposition in a cerebellar cell model of juvenile neuronal ceroid lipofuscinosis
T171	9-20	NN	denotes	trafficking
T174	21-24	CC	denotes	and
T175	25-38	JJ	denotes	mitochondrial
T176	39-52	NNS	denotes	abnormalities
T173	53-60	VBP	denotes	precede
T177	61-68	NN	denotes	subunit
T178	69-70	NN	denotes	c
T179	71-81	NN	denotes	deposition
T180	82-84	IN	denotes	in
T181	85-86	DT	denotes	a
T183	87-97	JJ	denotes	cerebellar
T184	98-102	NN	denotes	cell
T182	103-108	NN	denotes	model
T185	109-111	IN	denotes	of
T186	112-120	JJ	denotes	juvenile
T188	121-129	JJ	denotes	neuronal
T189	130-136	NN	denotes	ceroid
T187	137-151	NN	denotes	lipofuscinosis
T190	151-152	sentence	denotes
T191	162-172	sentence	denotes	Background
T192	162-172	NN	denotes	Background
T193	172-294	sentence	denotes	JNCL is a recessively inherited, childhood-onset neurodegenerative disease most-commonly caused by a ~1 kb CLN3 mutation.
T194	173-177	NN	denotes	JNCL
T195	178-180	VBZ	denotes	is
T196	181-182	DT	denotes	a
T198	183-194	RB	denotes	recessively
T199	195-204	VBN	denotes	inherited
T200	204-206	,	denotes	,
T201	206-215	NN	denotes	childhood
T203	215-216	HYPH	denotes	-
T202	216-221	NN	denotes	onset
T204	222-239	JJ	denotes	neurodegenerative
T197	240-247	NN	denotes	disease
T205	248-252	RBS	denotes	most
T207	252-253	HYPH	denotes	-
T206	253-261	RB	denotes	commonly
T208	262-268	VBN	denotes	caused
T209	269-271	IN	denotes	by
T210	272-273	DT	denotes	a
T212	274-275	SYM	denotes	~
T213	275-276	CD	denotes	1
T214	277-279	NN	denotes	kb
T215	280-284	NN	denotes	CLN3
T211	285-293	NN	denotes	mutation
T216	293-294	.	denotes	.
T217	294-431	sentence	denotes	The resulting loss of battenin activity leads to deposition of mitochondrial ATP synthase, subunit c and a specific loss of CNS neurons.
T218	295-298	DT	denotes	The
T220	299-308	JJ	denotes	resulting
T219	309-313	NN	denotes	loss
T222	314-316	IN	denotes	of
T223	317-325	NN	denotes	battenin
T224	326-334	NN	denotes	activity
T221	335-340	VBZ	denotes	leads
T225	341-343	IN	denotes	to
T226	344-354	NN	denotes	deposition
T227	355-357	IN	denotes	of
T228	358-371	JJ	denotes	mitochondrial
T230	372-375	NN	denotes	ATP
T229	376-384	NN	denotes	synthase
T231	384-386	,	denotes	,
T232	386-393	NN	denotes	subunit
T233	394-395	NN	denotes	c
T234	396-399	CC	denotes	and
T235	400-401	DT	denotes	a
T237	402-410	JJ	denotes	specific
T236	411-415	NN	denotes	loss
T238	416-418	IN	denotes	of
T239	419-422	NN	denotes	CNS
T240	423-430	NNS	denotes	neurons
T241	430-431	.	denotes	.
T242	431-580	sentence	denotes	We previously generated Cln3Δex7/8 knock-in mice, which replicate the common JNCL mutation, express mutant battenin and display JNCL-like pathology.
T243	432-434	PRP	denotes	We
T245	435-445	RB	denotes	previously
T244	446-455	VBD	denotes	generated
T246	456-464	NN	denotes	Cln3Δex7
T248	464-465	HYPH	denotes	/
T249	465-466	CD	denotes	8
T250	467-472	VB	denotes	knock
T251	472-473	HYPH	denotes	-
T252	473-475	RP	denotes	in
T247	476-480	NNS	denotes	mice
T253	480-482	,	denotes	,
T254	482-487	WDT	denotes	which
T255	488-497	VBP	denotes	replicate
T256	498-501	DT	denotes	the
T258	502-508	JJ	denotes	common
T259	509-513	NN	denotes	JNCL
T257	514-522	NN	denotes	mutation
T260	522-524	,	denotes	,
T261	524-531	VBP	denotes	express
T262	532-538	NN	denotes	mutant
T263	539-547	NN	denotes	battenin
T264	548-551	CC	denotes	and
T265	552-559	VBP	denotes	display
T266	560-564	NN	denotes	JNCL
T268	564-565	HYPH	denotes	-
T267	565-569	JJ	denotes	like
T269	570-579	NN	denotes	pathology
T270	579-580	.	denotes	.
T271	580-589	sentence	denotes	Results
T272	582-589	NNS	denotes	Results
T273	589-897	sentence	denotes	To elucidate the consequences of the common JNCL mutation in neuronal cells, we used P4 knock-in mouse cerebella to establish conditionally immortalized CbCln3 wild-type, heterozygous, and homozygous neuronal precursor cell lines, which can be differentiated into MAP-2 and NeuN-positive, neuron-like cells.
T274	590-592	TO	denotes	To
T275	593-602	VB	denotes	elucidate
T277	603-606	DT	denotes	the
T278	607-619	NNS	denotes	consequences
T279	620-622	IN	denotes	of
T280	623-626	DT	denotes	the
T282	627-633	JJ	denotes	common
T283	634-638	NN	denotes	JNCL
T281	639-647	NN	denotes	mutation
T284	648-650	IN	denotes	in
T285	651-659	JJ	denotes	neuronal
T286	660-665	NNS	denotes	cells
T287	665-667	,	denotes	,
T288	667-669	PRP	denotes	we
T276	670-674	VBD	denotes	used
T289	675-677	NN	denotes	P4
T291	678-683	VB	denotes	knock
T292	683-684	HYPH	denotes	-
T293	684-686	RP	denotes	in
T294	687-692	NN	denotes	mouse
T290	693-702	NNS	denotes	cerebella
T295	703-705	TO	denotes	to
T296	706-715	VB	denotes	establish
T297	716-729	RB	denotes	conditionally
T298	730-742	VBN	denotes	immortalized
T300	743-749	NN	denotes	CbCln3
T301	750-754	JJ	denotes	wild
T303	754-755	HYPH	denotes	-
T302	755-759	NN	denotes	type
T304	759-761	,	denotes	,
T305	761-773	JJ	denotes	heterozygous
T306	773-775	,	denotes	,
T307	775-778	CC	denotes	and
T308	779-789	JJ	denotes	homozygous
T309	790-798	JJ	denotes	neuronal
T310	799-808	NN	denotes	precursor
T311	809-813	NN	denotes	cell
T299	814-819	NNS	denotes	lines
T312	819-821	,	denotes	,
T313	821-826	WDT	denotes	which
T315	827-830	MD	denotes	can
T316	831-833	VB	denotes	be
T314	834-848	VBN	denotes	differentiated
T317	849-853	IN	denotes	into
T318	854-857	NN	denotes	MAP
T320	857-858	HYPH	denotes	-
T321	858-859	CD	denotes	2
T322	860-863	CC	denotes	and
T323	864-868	NN	denotes	NeuN
T324	868-869	HYPH	denotes	-
T319	869-877	JJ	denotes	positive
T326	877-879	,	denotes	,
T327	879-885	NN	denotes	neuron
T329	885-886	HYPH	denotes	-
T328	886-890	JJ	denotes	like
T325	891-896	NNS	denotes	cells
T330	896-897	.	denotes	.
T331	897-1034	sentence	denotes	Homozygous CbCln3Δex7/8 precursor cells express low levels of mutant battenin and, when aged at confluency, accumulate ATPase subunit c.
T332	898-908	JJ	denotes	Homozygous
T334	909-919	NN	denotes	CbCln3Δex7
T335	919-920	HYPH	denotes	/
T336	920-921	CD	denotes	8
T337	922-931	NN	denotes	precursor
T333	932-937	NNS	denotes	cells
T338	938-945	VBP	denotes	express
T339	946-949	JJ	denotes	low
T340	950-956	NNS	denotes	levels
T341	957-959	IN	denotes	of
T342	960-966	NN	denotes	mutant
T343	967-975	NN	denotes	battenin
T344	976-979	CC	denotes	and
T345	979-981	,	denotes	,
T346	981-985	WRB	denotes	when
T347	986-990	VBN	denotes	aged
T349	991-993	IN	denotes	at
T350	994-1004	NN	denotes	confluency
T351	1004-1006	,	denotes	,
T348	1006-1016	VBP	denotes	accumulate
T352	1017-1023	NN	denotes	ATPase
T354	1024-1031	NN	denotes	subunit
T353	1032-1033	NN	denotes	c
T355	1033-1034	.	denotes	.
T356	1034-1277	sentence	denotes	Recessive phenotypes are also observed at sub-confluent growth; cathepsin D transport and processing are altered, although enzyme activity is not significantly affected, lysosomal size and distribution are altered, and endocytosis is reduced.
T357	1035-1044	JJ	denotes	Recessive
T358	1045-1055	NNS	denotes	phenotypes
T360	1056-1059	VBP	denotes	are
T361	1060-1064	RB	denotes	also
T359	1065-1073	VBN	denotes	observed
T362	1074-1076	IN	denotes	at
T363	1077-1090	JJ	denotes	sub-confluent
T364	1091-1097	NN	denotes	growth
T365	1097-1098	:	denotes	;
T366	1099-1108	NN	denotes	cathepsin
T367	1109-1110	NN	denotes	D
T369	1111-1120	NN	denotes	transport
T370	1121-1124	CC	denotes	and
T371	1125-1135	NN	denotes	processing
T372	1136-1139	VBP	denotes	are
T368	1140-1147	VBN	denotes	altered
T373	1147-1149	,	denotes	,
T374	1149-1157	IN	denotes	although
T376	1158-1164	NN	denotes	enzyme
T377	1165-1173	NN	denotes	activity
T378	1174-1176	VBZ	denotes	is
T379	1177-1180	RB	denotes	not
T380	1181-1194	RB	denotes	significantly
T375	1195-1203	VBN	denotes	affected
T382	1203-1205	,	denotes	,
T383	1205-1214	JJ	denotes	lysosomal
T384	1215-1219	NN	denotes	size
T385	1220-1223	CC	denotes	and
T386	1224-1236	NN	denotes	distribution
T387	1237-1240	VBP	denotes	are
T381	1241-1248	VBN	denotes	altered
T388	1248-1250	,	denotes	,
T389	1250-1253	CC	denotes	and
T390	1254-1265	NN	denotes	endocytosis
T392	1266-1268	VBZ	denotes	is
T391	1269-1276	VBN	denotes	reduced
T393	1276-1277	.	denotes	.
T394	1277-1416	sentence	denotes	In addition, mitochondria are abnormally elongated, cellular ATP levels are decreased, and survival following oxidative stress is reduced.
T395	1278-1280	IN	denotes	In
T397	1281-1289	NN	denotes	addition
T398	1289-1291	,	denotes	,
T399	1291-1303	NNS	denotes	mitochondria
T396	1304-1307	VBP	denotes	are
T400	1308-1318	RB	denotes	abnormally
T401	1319-1328	JJ	denotes	elongated
T402	1328-1330	,	denotes	,
T403	1330-1338	JJ	denotes	cellular
T405	1339-1342	NN	denotes	ATP
T404	1343-1349	NNS	denotes	levels
T407	1350-1353	VBP	denotes	are
T406	1354-1363	VBN	denotes	decreased
T408	1363-1365	,	denotes	,
T409	1365-1368	CC	denotes	and
T410	1369-1377	NN	denotes	survival
T412	1378-1387	VBG	denotes	following
T413	1388-1397	JJ	denotes	oxidative
T414	1398-1404	NN	denotes	stress
T415	1405-1407	VBZ	denotes	is
T411	1408-1415	VBN	denotes	reduced
T416	1415-1416	.	denotes	.
T417	1416-1429	sentence	denotes	Conclusions
T418	1418-1429	NNS	denotes	Conclusions
T419	1429-1544	sentence	denotes	These findings reveal that battenin is required for intracellular membrane trafficking and mitochondrial function.
T420	1430-1435	DT	denotes	These
T421	1436-1444	NNS	denotes	findings
T422	1445-1451	VBP	denotes	reveal
T423	1452-1456	IN	denotes	that
T425	1457-1465	NN	denotes	battenin
T424	1466-1468	VBZ	denotes	is
T426	1469-1477	JJ	denotes	required
T427	1478-1481	IN	denotes	for
T428	1482-1495	JJ	denotes	intracellular
T430	1496-1504	NN	denotes	membrane
T429	1505-1516	NN	denotes	trafficking
T431	1517-1520	CC	denotes	and
T432	1521-1534	JJ	denotes	mitochondrial
T433	1535-1543	NN	denotes	function
T434	1543-1544	.	denotes	.
T435	1544-1678	sentence	denotes	Moreover, these deficiencies are likely to be early events in the JNCL disease process and may particularly impact neuronal survival.
T436	1545-1553	RB	denotes	Moreover
T438	1553-1555	,	denotes	,
T439	1555-1560	DT	denotes	these
T440	1561-1573	NNS	denotes	deficiencies
T437	1574-1577	VBP	denotes	are
T441	1578-1584	JJ	denotes	likely
T442	1585-1587	TO	denotes	to
T443	1588-1590	VB	denotes	be
T444	1591-1596	JJ	denotes	early
T445	1597-1603	NNS	denotes	events
T446	1604-1606	IN	denotes	in
T447	1607-1610	DT	denotes	the
T449	1611-1615	NN	denotes	JNCL
T450	1616-1623	NN	denotes	disease
T448	1624-1631	NN	denotes	process
T451	1632-1635	CC	denotes	and
T452	1636-1639	MD	denotes	may
T454	1640-1652	RB	denotes	particularly
T453	1653-1659	VB	denotes	impact
T455	1660-1668	JJ	denotes	neuronal
T456	1669-1677	NN	denotes	survival
T457	1677-1678	.	denotes	.
T696	1691-1699	JJ	denotes	Juvenile
T698	1700-1708	JJ	denotes	neuronal
T699	1709-1715	NN	denotes	ceroid
T697	1716-1730	NN	denotes	lipofuscinosis
T701	1731-1732	-LRB-	denotes	(
T702	1732-1736	NN	denotes	JNCL
T703	1736-1737	-RRB-	denotes	)
T704	1737-1739	,	denotes	,
T705	1739-1741	CC	denotes	or
T706	1742-1748	NNP	denotes	Batten
T707	1749-1756	NN	denotes	disease
T708	1756-1758	,	denotes	,
T700	1758-1760	VBZ	denotes	is
T709	1761-1762	DT	denotes	a
T711	1763-1774	RB	denotes	recessively
T712	1775-1784	VBN	denotes	inherited
T713	1785-1794	NN	denotes	childhood
T714	1794-1795	HYPH	denotes	-
T715	1795-1800	JJ	denotes	onset
T716	1801-1818	JJ	denotes	neurodegenerative
T710	1819-1827	NN	denotes	disorder
T717	1828-1841	VBN	denotes	characterized
T718	1842-1844	IN	denotes	by
T719	1845-1856	JJ	denotes	progressive
T720	1857-1866	NN	denotes	blindness
T721	1866-1868	,	denotes	,
T722	1868-1876	NNS	denotes	seizures
T723	1876-1878	,	denotes	,
T724	1878-1883	NN	denotes	motor
T726	1884-1887	CC	denotes	and
T727	1888-1897	JJ	denotes	cognitive
T725	1898-1905	NN	denotes	decline
T728	1905-1907	,	denotes	,
T729	1907-1910	CC	denotes	and
T730	1911-1916	JJ	denotes	early
T731	1917-1922	NN	denotes	death
T732	1923-1924	-LRB-	denotes	[
T733	1924-1925	CD	denotes	1
T734	1925-1926	-RRB-	denotes	]
T735	1926-1927	.	denotes	.
T736	1927-2159	sentence	denotes	The primary genetic defect (>80% disease chromosomes) leading to JNCL is a 1.02 kb genomic DNA deletion in the CLN3 gene, which eliminates exons 7 and 8 and surrounding intronic DNA, predicting a non-functional protein product [2].
T737	1928-1931	DT	denotes	The
T739	1932-1939	JJ	denotes	primary
T740	1940-1947	JJ	denotes	genetic
T738	1948-1954	NN	denotes	defect
T742	1955-1956	-LRB-	denotes	(
T744	1956-1957	SYM	denotes	>
T745	1957-1959	CD	denotes	80
T746	1959-1960	NN	denotes	%
T747	1961-1968	NN	denotes	disease
T743	1969-1980	NNS	denotes	chromosomes
T748	1980-1981	-RRB-	denotes	)
T749	1982-1989	VBG	denotes	leading
T750	1990-1992	IN	denotes	to
T751	1993-1997	NN	denotes	JNCL
T741	1998-2000	VBZ	denotes	is
T752	2001-2002	DT	denotes	a
T754	2003-2007	CD	denotes	1.02
T755	2008-2010	NN	denotes	kb
T756	2011-2018	JJ	denotes	genomic
T757	2019-2022	NN	denotes	DNA
T753	2023-2031	NN	denotes	deletion
T758	2032-2034	IN	denotes	in
T759	2035-2038	DT	denotes	the
T761	2039-2043	NN	denotes	CLN3
T760	2044-2048	NN	denotes	gene
T762	2048-2050	,	denotes	,
T763	2050-2055	WDT	denotes	which
T764	2056-2066	VBZ	denotes	eliminates
T765	2067-2072	NNS	denotes	exons
T766	2073-2074	CD	denotes	7
T767	2075-2078	CC	denotes	and
T768	2079-2080	CD	denotes	8
T769	2081-2084	CC	denotes	and
T770	2085-2096	JJ	denotes	surrounding
T772	2097-2105	JJ	denotes	intronic
T771	2106-2109	NN	denotes	DNA
T773	2109-2111	,	denotes	,
T774	2111-2121	VBG	denotes	predicting
T775	2122-2123	DT	denotes	a
T777	2124-2138	JJ	denotes	non-functional
T778	2139-2146	NN	denotes	protein
T776	2147-2154	NN	denotes	product
T779	2155-2156	-LRB-	denotes	[
T780	2156-2157	CD	denotes	2
T781	2157-2158	-RRB-	denotes	]
T782	2158-2159	.	denotes	.
T783	2159-2340	sentence	denotes	The pathological hallmark of JNCL is autofluorescent ceroid lipofuscin deposits within autolysosomes that are enriched in subunit c of the mitochondrial ATP synthase complex [3-5].
T784	2160-2163	DT	denotes	The
T786	2164-2176	JJ	denotes	pathological
T785	2177-2185	NN	denotes	hallmark
T788	2186-2188	IN	denotes	of
T789	2189-2193	NN	denotes	JNCL
T787	2194-2196	VBZ	denotes	is
T790	2197-2212	JJ	denotes	autofluorescent
T792	2213-2219	NN	denotes	ceroid
T793	2220-2230	NN	denotes	lipofuscin
T791	2231-2239	NNS	denotes	deposits
T794	2240-2246	IN	denotes	within
T795	2247-2260	NNS	denotes	autolysosomes
T796	2261-2265	WDT	denotes	that
T797	2266-2269	VBP	denotes	are
T798	2270-2278	JJ	denotes	enriched
T799	2279-2281	IN	denotes	in
T800	2282-2289	NN	denotes	subunit
T801	2290-2291	NN	denotes	c
T802	2292-2294	IN	denotes	of
T803	2295-2298	DT	denotes	the
T805	2299-2312	JJ	denotes	mitochondrial
T807	2313-2316	NN	denotes	ATP
T806	2317-2325	NN	denotes	synthase
T804	2326-2333	NN	denotes	complex
T808	2334-2335	-LRB-	denotes	[
T809	2335-2336	CD	denotes	3
T810	2336-2337	SYM	denotes	-
T811	2337-2338	CD	denotes	5
T812	2338-2339	-RRB-	denotes	]
T813	2339-2340	.	denotes	.
T814	2340-2476	sentence	denotes	Remarkably, these deposits are not only found in CNS neurons but are also abundant in non-neuronal cells outside of the nervous system.
T815	2341-2351	RB	denotes	Remarkably
T817	2351-2353	,	denotes	,
T818	2353-2358	DT	denotes	these
T819	2359-2367	NNS	denotes	deposits
T820	2368-2371	VBP	denotes	are
T821	2372-2375	RB	denotes	not
T822	2376-2380	RB	denotes	only
T816	2381-2386	VBN	denotes	found
T823	2387-2389	IN	denotes	in
T824	2390-2393	NN	denotes	CNS
T825	2394-2401	NNS	denotes	neurons
T826	2402-2405	CC	denotes	but
T827	2406-2409	VBP	denotes	are
T828	2410-2414	RB	denotes	also
T829	2415-2423	JJ	denotes	abundant
T830	2424-2426	IN	denotes	in
T831	2427-2439	JJ	denotes	non-neuronal
T832	2440-2445	NNS	denotes	cells
T833	2446-2453	IN	denotes	outside
T834	2454-2456	IN	denotes	of
T835	2457-2460	DT	denotes	the
T837	2461-2468	JJ	denotes	nervous
T836	2469-2475	NN	denotes	system
T838	2475-2476	.	denotes	.
T839	2476-2640	sentence	denotes	The relationship of subunit c deposits to the JNCL disease process, and the underlying reason for the neuronal specificity of the disease remain poorly understood.
T840	2477-2480	DT	denotes	The
T841	2481-2493	NN	denotes	relationship
T843	2494-2496	IN	denotes	of
T844	2497-2504	NN	denotes	subunit
T845	2505-2506	NN	denotes	c
T846	2507-2515	NNS	denotes	deposits
T847	2516-2518	IN	denotes	to
T848	2519-2522	DT	denotes	the
T850	2523-2527	NN	denotes	JNCL
T851	2528-2535	NN	denotes	disease
T849	2536-2543	NN	denotes	process
T852	2543-2545	,	denotes	,
T853	2545-2548	CC	denotes	and
T854	2549-2552	DT	denotes	the
T856	2553-2563	JJ	denotes	underlying
T855	2564-2570	NN	denotes	reason
T857	2571-2574	IN	denotes	for
T858	2575-2578	DT	denotes	the
T860	2579-2587	JJ	denotes	neuronal
T859	2588-2599	NN	denotes	specificity
T861	2600-2602	IN	denotes	of
T862	2603-2606	DT	denotes	the
T863	2607-2614	NN	denotes	disease
T842	2615-2621	VBP	denotes	remain
T864	2622-2628	RB	denotes	poorly
T865	2629-2639	VBN	denotes	understood
T866	2639-2640	.	denotes	.
T867	2640-2854	sentence	denotes	The CLN3-encoded protein (battenin, also called CLN3 or cln3 p) is a highly conserved, ubiquitously expressed, multi-pass membrane protein [6] that localizes to the lysosome and other vesicular compartments [7-9].
T868	2641-2644	DT	denotes	The
T870	2645-2649	NN	denotes	CLN3
T872	2649-2650	HYPH	denotes	-
T871	2650-2657	VBN	denotes	encoded
T869	2658-2665	NN	denotes	protein
T874	2666-2667	-LRB-	denotes	(
T875	2667-2675	NN	denotes	battenin
T876	2675-2677	,	denotes	,
T877	2677-2681	RB	denotes	also
T878	2682-2688	VBN	denotes	called
T879	2689-2693	NN	denotes	CLN3
T880	2694-2696	CC	denotes	or
T881	2697-2701	NN	denotes	cln3
T882	2702-2703	NN	denotes	p
T883	2703-2704	-RRB-	denotes	)
T873	2705-2707	VBZ	denotes	is
T884	2708-2709	DT	denotes	a
T886	2710-2716	RB	denotes	highly
T887	2717-2726	JJ	denotes	conserved
T888	2726-2728	,	denotes	,
T889	2728-2740	RB	denotes	ubiquitously
T890	2741-2750	VBN	denotes	expressed
T891	2750-2752	,	denotes	,
T892	2752-2762	NN	denotes	multi-pass
T893	2763-2771	NN	denotes	membrane
T885	2772-2779	NN	denotes	protein
T894	2780-2781	-LRB-	denotes	[
T895	2781-2782	CD	denotes	6
T896	2782-2783	-RRB-	denotes	]
T897	2784-2788	WDT	denotes	that
T898	2789-2798	VBZ	denotes	localizes
T899	2799-2801	IN	denotes	to
T900	2802-2805	DT	denotes	the
T901	2806-2814	NN	denotes	lysosome
T902	2815-2818	CC	denotes	and
T903	2819-2824	JJ	denotes	other
T905	2825-2834	JJ	denotes	vesicular
T904	2835-2847	NNS	denotes	compartments
T906	2848-2849	-LRB-	denotes	[
T907	2849-2850	CD	denotes	7
T908	2850-2851	SYM	denotes	-
T909	2851-2852	CD	denotes	9
T910	2852-2853	-RRB-	denotes	]
T911	2853-2854	.	denotes	.
T912	2854-3036	sentence	denotes	Battenin function remains to be elucidated, although studies of btn1, the yeast CLN3 ortholog, have implicated battenin in lysosomal pH homeostasis and amino acid transport [10,11].
T913	2855-2863	NN	denotes	Battenin
T914	2864-2872	NN	denotes	function
T915	2873-2880	VBZ	denotes	remains
T916	2881-2883	TO	denotes	to
T918	2884-2886	VB	denotes	be
T917	2887-2897	VBN	denotes	elucidated
T919	2897-2899	,	denotes	,
T920	2899-2907	IN	denotes	although
T922	2908-2915	NNS	denotes	studies
T923	2916-2918	IN	denotes	of
T924	2919-2923	NN	denotes	btn1
T925	2923-2925	,	denotes	,
T926	2925-2928	DT	denotes	the
T928	2929-2934	NN	denotes	yeast
T929	2935-2939	NN	denotes	CLN3
T927	2940-2948	NN	denotes	ortholog
T930	2948-2950	,	denotes	,
T931	2950-2954	VBP	denotes	have
T921	2955-2965	VBN	denotes	implicated
T932	2966-2974	NN	denotes	battenin
T933	2975-2977	IN	denotes	in
T934	2978-2987	JJ	denotes	lysosomal
T936	2988-2990	NN	denotes	pH
T935	2991-3002	NN	denotes	homeostasis
T937	3003-3006	CC	denotes	and
T938	3007-3012	NN	denotes	amino
T939	3013-3017	NN	denotes	acid
T940	3018-3027	NN	denotes	transport
T941	3028-3029	-LRB-	denotes	[
T943	3029-3031	CD	denotes	10
T944	3031-3032	,	denotes	,
T942	3032-3034	CD	denotes	11
T945	3034-3035	-RRB-	denotes	]
T946	3035-3036	.	denotes	.
T947	3036-3152	sentence	denotes	To explore JNCL pathogenesis and battenin function, we previously generated a genetically precise JNCL mouse model.
T948	3037-3039	TO	denotes	To
T949	3040-3047	VB	denotes	explore
T951	3048-3052	NN	denotes	JNCL
T952	3053-3065	NN	denotes	pathogenesis
T953	3066-3069	CC	denotes	and
T954	3070-3078	NN	denotes	battenin
T955	3079-3087	NN	denotes	function
T956	3087-3089	,	denotes	,
T957	3089-3091	PRP	denotes	we
T958	3092-3102	RB	denotes	previously
T950	3103-3112	VBD	denotes	generated
T959	3113-3114	DT	denotes	a
T961	3115-3126	RB	denotes	genetically
T962	3127-3134	JJ	denotes	precise
T963	3135-3139	NN	denotes	JNCL
T964	3140-3145	NN	denotes	mouse
T960	3146-3151	NN	denotes	model
T965	3151-3152	.	denotes	.
T966	3152-3335	sentence	denotes	Cln3Δex7/8 knock-in mice harbor the ~1 kb common JNCL mutation and express a non-truncated mutant battenin isoform that is detectable with antibodies recognizing C-terminal epitopes.
T967	3153-3161	NN	denotes	Cln3Δex7
T969	3161-3162	HYPH	denotes	/
T970	3162-3163	CD	denotes	8
T971	3164-3169	VB	denotes	knock
T972	3169-3170	HYPH	denotes	-
T973	3170-3172	RP	denotes	in
T968	3173-3177	NNS	denotes	mice
T974	3178-3184	VBP	denotes	harbor
T975	3185-3188	DT	denotes	the
T977	3189-3190	SYM	denotes	~
T978	3190-3191	CD	denotes	1
T979	3192-3194	NN	denotes	kb
T980	3195-3201	JJ	denotes	common
T981	3202-3206	NN	denotes	JNCL
T976	3207-3215	NN	denotes	mutation
T982	3216-3219	CC	denotes	and
T983	3220-3227	VBP	denotes	express
T984	3228-3229	DT	denotes	a
T986	3230-3243	JJ	denotes	non-truncated
T987	3244-3250	NN	denotes	mutant
T988	3251-3259	NN	denotes	battenin
T985	3260-3267	NN	denotes	isoform
T989	3268-3272	WDT	denotes	that
T990	3273-3275	VBZ	denotes	is
T991	3276-3286	JJ	denotes	detectable
T992	3287-3291	IN	denotes	with
T993	3292-3302	NNS	denotes	antibodies
T994	3303-3314	VBG	denotes	recognizing
T995	3315-3316	NN	denotes	C
T997	3316-3317	HYPH	denotes	-
T996	3317-3325	JJ	denotes	terminal
T998	3326-3334	NNS	denotes	epitopes
T999	3334-3335	.	denotes	.
T1000	3335-3546	sentence	denotes	Homozygous Cln3Δex7/8 knock-in mice exhibit a progressive JNCL-like disease, with perinatal onset of subunit c deposition in many cell types and later onset of neuronal dysfunction and behavioral deficits [12].
T1001	3336-3346	JJ	denotes	Homozygous
T1003	3347-3355	NN	denotes	Cln3Δex7
T1004	3355-3356	HYPH	denotes	/
T1005	3356-3357	CD	denotes	8
T1006	3358-3363	VB	denotes	knock
T1007	3363-3364	HYPH	denotes	-
T1008	3364-3366	RP	denotes	in
T1002	3367-3371	NNS	denotes	mice
T1009	3372-3379	VBP	denotes	exhibit
T1010	3380-3381	DT	denotes	a
T1012	3382-3393	JJ	denotes	progressive
T1013	3394-3398	NN	denotes	JNCL
T1015	3398-3399	HYPH	denotes	-
T1014	3399-3403	JJ	denotes	like
T1011	3404-3411	NN	denotes	disease
T1016	3411-3413	,	denotes	,
T1017	3413-3417	IN	denotes	with
T1018	3418-3427	JJ	denotes	perinatal
T1019	3428-3433	NN	denotes	onset
T1020	3434-3436	IN	denotes	of
T1021	3437-3444	NN	denotes	subunit
T1022	3445-3446	NN	denotes	c
T1023	3447-3457	NN	denotes	deposition
T1024	3458-3460	IN	denotes	in
T1025	3461-3465	JJ	denotes	many
T1027	3466-3470	NN	denotes	cell
T1026	3471-3476	NNS	denotes	types
T1028	3477-3480	CC	denotes	and
T1029	3481-3486	JJ	denotes	later
T1030	3487-3492	NN	denotes	onset
T1031	3493-3495	IN	denotes	of
T1032	3496-3504	JJ	denotes	neuronal
T1033	3505-3516	NN	denotes	dysfunction
T1034	3517-3520	CC	denotes	and
T1035	3521-3531	JJ	denotes	behavioral
T1036	3532-3540	NNS	denotes	deficits
T1037	3541-3542	-LRB-	denotes	[
T1038	3542-3544	CD	denotes	12
T1039	3544-3545	-RRB-	denotes	]
T1040	3545-3546	.	denotes	.
T1041	3546-3705	sentence	denotes	These findings suggest that the major JNCL defect leads to abnormal turnover of mitochondrial subunit c, in a manner that selectively compromises CNS neurons.
T1042	3547-3552	DT	denotes	These
T1043	3553-3561	NNS	denotes	findings
T1044	3562-3569	VBP	denotes	suggest
T1045	3570-3574	IN	denotes	that
T1047	3575-3578	DT	denotes	the
T1049	3579-3584	JJ	denotes	major
T1050	3585-3589	NN	denotes	JNCL
T1048	3590-3596	NN	denotes	defect
T1046	3597-3602	VBZ	denotes	leads
T1051	3603-3605	IN	denotes	to
T1052	3606-3614	JJ	denotes	abnormal
T1053	3615-3623	NN	denotes	turnover
T1054	3624-3626	IN	denotes	of
T1055	3627-3640	JJ	denotes	mitochondrial
T1057	3641-3648	NN	denotes	subunit
T1056	3649-3650	NN	denotes	c
T1058	3650-3652	,	denotes	,
T1059	3652-3654	IN	denotes	in
T1060	3655-3656	DT	denotes	a
T1061	3657-3663	NN	denotes	manner
T1062	3664-3668	WDT	denotes	that
T1064	3669-3680	RB	denotes	selectively
T1063	3681-3692	VBZ	denotes	compromises
T1065	3693-3696	NN	denotes	CNS
T1066	3697-3704	NNS	denotes	neurons
T1067	3704-3705	.	denotes	.
T1068	3705-3837	sentence	denotes	Currently, there is no suitable neuronal cell system to investigate the impact of the common JNCL mutation on biological processes.
T1069	3706-3715	RB	denotes	Currently
T1071	3715-3717	,	denotes	,
T1072	3717-3722	EX	denotes	there
T1070	3723-3725	VBZ	denotes	is
T1073	3726-3728	DT	denotes	no
T1075	3729-3737	JJ	denotes	suitable
T1076	3738-3746	JJ	denotes	neuronal
T1077	3747-3751	NN	denotes	cell
T1074	3752-3758	NN	denotes	system
T1078	3759-3761	TO	denotes	to
T1079	3762-3773	VB	denotes	investigate
T1080	3774-3777	DT	denotes	the
T1081	3778-3784	NN	denotes	impact
T1082	3785-3787	IN	denotes	of
T1083	3788-3791	DT	denotes	the
T1085	3792-3798	JJ	denotes	common
T1086	3799-3803	NN	denotes	JNCL
T1084	3804-3812	NN	denotes	mutation
T1087	3813-3815	IN	denotes	on
T1088	3816-3826	JJ	denotes	biological
T1089	3827-3836	NNS	denotes	processes
T1090	3836-3837	.	denotes	.
T1091	3837-3940	sentence	denotes	Therefore, we have established cerebellar neuronal precursor cell lines from Cln3Δex7/8 knock-in mice.
T1092	3838-3847	RB	denotes	Therefore
T1094	3847-3849	,	denotes	,
T1095	3849-3851	PRP	denotes	we
T1096	3852-3856	VBP	denotes	have
T1093	3857-3868	VBN	denotes	established
T1097	3869-3879	JJ	denotes	cerebellar
T1099	3880-3888	JJ	denotes	neuronal
T1100	3889-3898	NN	denotes	precursor
T1101	3899-3903	NN	denotes	cell
T1098	3904-3909	NNS	denotes	lines
T1102	3910-3914	IN	denotes	from
T1103	3915-3923	NN	denotes	Cln3Δex7
T1105	3923-3924	HYPH	denotes	/
T1106	3924-3925	CD	denotes	8
T1107	3926-3931	VB	denotes	knock
T1108	3931-3932	HYPH	denotes	-
T1109	3932-3934	RP	denotes	in
T1104	3935-3939	NNS	denotes	mice
T1110	3939-3940	.	denotes	.
T1111	3940-4162	sentence	denotes	Homozygous CbCln3Δex7/8 cells exhibit pathological hallmarks of the disease, and a survey of membrane organelles revealed membrane trafficking defects and mitochondrial dysfunction in homozygous mutant CbCln3Δex7/8 cells.
T1112	3941-3951	JJ	denotes	Homozygous
T1114	3952-3962	NN	denotes	CbCln3Δex7
T1115	3962-3963	HYPH	denotes	/
T1116	3963-3964	CD	denotes	8
T1113	3965-3970	NNS	denotes	cells
T1117	3971-3978	VBP	denotes	exhibit
T1118	3979-3991	JJ	denotes	pathological
T1119	3992-4001	NNS	denotes	hallmarks
T1120	4002-4004	IN	denotes	of
T1121	4005-4008	DT	denotes	the
T1122	4009-4016	NN	denotes	disease
T1123	4016-4018	,	denotes	,
T1124	4018-4021	CC	denotes	and
T1125	4022-4023	DT	denotes	a
T1126	4024-4030	NN	denotes	survey
T1128	4031-4033	IN	denotes	of
T1129	4034-4042	NN	denotes	membrane
T1130	4043-4053	NNS	denotes	organelles
T1127	4054-4062	VBD	denotes	revealed
T1131	4063-4071	NN	denotes	membrane
T1132	4072-4083	VBG	denotes	trafficking
T1133	4084-4091	NNS	denotes	defects
T1134	4092-4095	CC	denotes	and
T1135	4096-4109	JJ	denotes	mitochondrial
T1136	4110-4121	NN	denotes	dysfunction
T1137	4122-4124	IN	denotes	in
T1138	4125-4135	JJ	denotes	homozygous
T1139	4136-4142	NN	denotes	mutant
T1141	4143-4153	NN	denotes	CbCln3Δex7
T1142	4153-4154	HYPH	denotes	/
T1143	4154-4155	CD	denotes	8
T1140	4156-4161	NNS	denotes	cells
T1144	4161-4162	.	denotes	.
T1271	4173-4183	NN	denotes	Generation
T1272	4184-4186	IN	denotes	of
T1273	4187-4188	DT	denotes	a
T1275	4189-4200	RB	denotes	genetically
T1276	4201-4208	JJ	denotes	precise
T1277	4209-4219	JJ	denotes	cerebellar
T1278	4220-4224	NN	denotes	JNCL
T1279	4225-4229	NN	denotes	cell
T1274	4230-4235	NN	denotes	model
T1280	4235-4468	sentence	denotes	To generate a precise genetic, neuron-derived JNCL cell culture system, we immortalized granule neurons cultured from postnatal day 4 (P4) cerebella of homozygous and heterozygous Cln3Δex7/8 knock-in mice, and wild-type littermates.
T1281	4236-4238	TO	denotes	To
T1282	4239-4247	VB	denotes	generate
T1284	4248-4249	DT	denotes	a
T1286	4250-4257	JJ	denotes	precise
T1287	4258-4265	JJ	denotes	genetic
T1288	4265-4267	,	denotes	,
T1289	4267-4273	NN	denotes	neuron
T1291	4273-4274	HYPH	denotes	-
T1290	4274-4281	VBN	denotes	derived
T1292	4282-4286	NN	denotes	JNCL
T1294	4287-4291	NN	denotes	cell
T1293	4292-4299	NN	denotes	culture
T1285	4300-4306	NN	denotes	system
T1295	4306-4308	,	denotes	,
T1296	4308-4310	PRP	denotes	we
T1283	4311-4323	VBD	denotes	immortalized
T1297	4324-4331	NN	denotes	granule
T1298	4332-4339	NNS	denotes	neurons
T1299	4340-4348	VBN	denotes	cultured
T1300	4349-4353	IN	denotes	from
T1301	4354-4363	JJ	denotes	postnatal
T1302	4364-4367	NN	denotes	day
T1304	4368-4369	CD	denotes	4
T1305	4370-4371	-LRB-	denotes	(
T1306	4371-4373	NN	denotes	P4
T1307	4373-4374	-RRB-	denotes	)
T1303	4375-4384	NNS	denotes	cerebella
T1308	4385-4387	IN	denotes	of
T1309	4388-4398	JJ	denotes	homozygous
T1311	4399-4402	CC	denotes	and
T1312	4403-4415	JJ	denotes	heterozygous
T1313	4416-4424	NN	denotes	Cln3Δex7
T1314	4424-4425	HYPH	denotes	/
T1315	4425-4426	CD	denotes	8
T1316	4427-4432	VB	denotes	knock
T1317	4432-4433	HYPH	denotes	-
T1318	4433-4435	RP	denotes	in
T1310	4436-4440	NNS	denotes	mice
T1319	4440-4442	,	denotes	,
T1320	4442-4445	CC	denotes	and
T1321	4446-4450	JJ	denotes	wild
T1323	4450-4451	HYPH	denotes	-
T1322	4451-4455	NN	denotes	type
T1324	4456-4467	NNS	denotes	littermates
T1325	4467-4468	.	denotes	.
T1326	4468-4641	sentence	denotes	Primary cell cultures enriched for granule neurons were transduced with retroviral vector bearing a selection cassette and temperature-sensitive tsA58 SV40 large T antigen.
T1327	4469-4476	JJ	denotes	Primary
T1329	4477-4481	NN	denotes	cell
T1328	4482-4490	NNS	denotes	cultures
T1331	4491-4499	VBN	denotes	enriched
T1332	4500-4503	IN	denotes	for
T1333	4504-4511	NN	denotes	granule
T1334	4512-4519	NNS	denotes	neurons
T1335	4520-4524	VBD	denotes	were
T1330	4525-4535	VBN	denotes	transduced
T1336	4536-4540	IN	denotes	with
T1337	4541-4551	JJ	denotes	retroviral
T1338	4552-4558	NN	denotes	vector
T1339	4559-4566	VBG	denotes	bearing
T1340	4567-4568	DT	denotes	a
T1342	4569-4578	NN	denotes	selection
T1341	4579-4587	NN	denotes	cassette
T1343	4588-4591	CC	denotes	and
T1344	4592-4603	NN	denotes	temperature
T1346	4603-4604	HYPH	denotes	-
T1345	4604-4613	JJ	denotes	sensitive
T1348	4614-4619	NN	denotes	tsA58
T1349	4620-4624	NN	denotes	SV40
T1350	4625-4630	JJ	denotes	large
T1351	4631-4632	NN	denotes	T
T1347	4633-4640	NN	denotes	antigen
T1352	4640-4641	.	denotes	.
T1353	4641-4852	sentence	denotes	Growth in G418 containing medium at the permissive temperature (33°C) allowed for selection and isolation of multiple clonal nestin-positive (Fig. 1a), and GFAP-negative (Fig. 1b), cell lines for each genotype.
T1354	4642-4648	NN	denotes	Growth
T1356	4649-4651	IN	denotes	in
T1357	4652-4656	NN	denotes	G418
T1358	4657-4667	VBG	denotes	containing
T1359	4668-4674	NN	denotes	medium
T1360	4675-4677	IN	denotes	at
T1361	4678-4681	DT	denotes	the
T1363	4682-4692	JJ	denotes	permissive
T1362	4693-4704	NN	denotes	temperature
T1364	4705-4706	-LRB-	denotes	(
T1365	4706-4708	CD	denotes	33
T1366	4708-4710	NN	denotes	°C
T1367	4710-4711	-RRB-	denotes	)
T1355	4712-4719	VBN	denotes	allowed
T1368	4720-4723	IN	denotes	for
T1369	4724-4733	NN	denotes	selection
T1370	4734-4737	CC	denotes	and
T1371	4738-4747	NN	denotes	isolation
T1372	4748-4750	IN	denotes	of
T1373	4751-4759	JJ	denotes	multiple
T1375	4760-4766	JJ	denotes	clonal
T1376	4767-4773	NN	denotes	nestin
T1378	4773-4774	HYPH	denotes	-
T1377	4774-4782	JJ	denotes	positive
T1379	4783-4784	-LRB-	denotes	(
T1381	4784-4788	NN	denotes	Fig.
T1380	4789-4791	NN	denotes	1a
T1382	4791-4792	-RRB-	denotes	)
T1383	4792-4794	,	denotes	,
T1384	4794-4797	CC	denotes	and
T1385	4798-4802	NN	denotes	GFAP
T1387	4802-4803	HYPH	denotes	-
T1386	4803-4811	JJ	denotes	negative
T1388	4812-4813	-LRB-	denotes	(
T1390	4813-4817	NN	denotes	Fig.
T1389	4818-4820	NN	denotes	1b
T1391	4820-4821	-RRB-	denotes	)
T1392	4821-4823	,	denotes	,
T1393	4823-4827	NN	denotes	cell
T1374	4828-4833	NNS	denotes	lines
T1394	4834-4837	IN	denotes	for
T1395	4838-4842	DT	denotes	each
T1396	4843-4851	NN	denotes	genotype
T1397	4851-4852	.	denotes	.
T1398	4852-4969	sentence	denotes	No genotype specific differences were observed in cellular morphology or doubling time (~46 hours) (data not shown).
T1399	4853-4855	DT	denotes	No
T1401	4856-4864	NN	denotes	genotype
T1402	4865-4873	JJ	denotes	specific
T1400	4874-4885	NNS	denotes	differences
T1404	4886-4890	VBD	denotes	were
T1403	4891-4899	VBN	denotes	observed
T1405	4900-4902	IN	denotes	in
T1406	4903-4911	JJ	denotes	cellular
T1407	4912-4922	NN	denotes	morphology
T1408	4923-4925	CC	denotes	or
T1409	4926-4934	NN	denotes	doubling
T1410	4935-4939	NN	denotes	time
T1411	4940-4941	-LRB-	denotes	(
T1412	4941-4942	SYM	denotes	~
T1413	4942-4944	CD	denotes	46
T1414	4945-4950	NNS	denotes	hours
T1415	4950-4951	-RRB-	denotes	)
T1416	4952-4953	-LRB-	denotes	(
T1418	4953-4957	NNS	denotes	data
T1419	4958-4961	RB	denotes	not
T1417	4962-4967	VBN	denotes	shown
T1420	4967-4968	-RRB-	denotes	)
T1421	4968-4969	.	denotes	.
T1422	4969-5139	sentence	denotes	As expected, SV40 large T antigen expression was rapidly lost and cell division ceased when cells were shifted to the non-permissive temperature (39°C) (data not shown).
T1423	4970-4972	IN	denotes	As
T1424	4973-4981	VBN	denotes	expected
T1426	4981-4983	,	denotes	,
T1427	4983-4987	NN	denotes	SV40
T1429	4988-4993	JJ	denotes	large
T1430	4994-4995	NN	denotes	T
T1431	4996-5003	NN	denotes	antigen
T1428	5004-5014	NN	denotes	expression
T1432	5015-5018	VBD	denotes	was
T1433	5019-5026	RB	denotes	rapidly
T1425	5027-5031	VBN	denotes	lost
T1434	5032-5035	CC	denotes	and
T1435	5036-5040	NN	denotes	cell
T1436	5041-5049	NN	denotes	division
T1437	5050-5056	VBD	denotes	ceased
T1438	5057-5061	WRB	denotes	when
T1440	5062-5067	NNS	denotes	cells
T1441	5068-5072	VBD	denotes	were
T1439	5073-5080	VBN	denotes	shifted
T1442	5081-5083	IN	denotes	to
T1443	5084-5087	DT	denotes	the
T1445	5088-5102	JJ	denotes	non-permissive
T1444	5103-5114	NN	denotes	temperature
T1446	5115-5116	-LRB-	denotes	(
T1447	5116-5118	CD	denotes	39
T1448	5118-5120	NN	denotes	°C
T1449	5120-5121	-RRB-	denotes	)
T1450	5122-5123	-LRB-	denotes	(
T1452	5123-5127	NNS	denotes	data
T1453	5128-5131	RB	denotes	not
T1451	5132-5137	VBN	denotes	shown
T1454	5137-5138	-RRB-	denotes	)
T1455	5138-5139	.	denotes	.
T1456	5139-5416	sentence	denotes	Upon addition of neuronal differentiation cocktail, precursor cells became neuron-like in morphology and exhibited decreased nestin expression (data not shown) and increased MAP2 and NeuN expression (Fig. 1c,1d), but not expression of the Purkinje marker, calbindin (Fig. 1e).
T1457	5140-5144	IN	denotes	Upon
T1459	5145-5153	NN	denotes	addition
T1460	5154-5156	IN	denotes	of
T1461	5157-5165	JJ	denotes	neuronal
T1462	5166-5181	NN	denotes	differentiation
T1463	5182-5190	NN	denotes	cocktail
T1464	5190-5192	,	denotes	,
T1465	5192-5201	NN	denotes	precursor
T1466	5202-5207	NNS	denotes	cells
T1458	5208-5214	VBD	denotes	became
T1467	5215-5221	NN	denotes	neuron
T1469	5221-5222	HYPH	denotes	-
T1468	5222-5226	JJ	denotes	like
T1470	5227-5229	IN	denotes	in
T1471	5230-5240	NN	denotes	morphology
T1472	5241-5244	CC	denotes	and
T1473	5245-5254	VBD	denotes	exhibited
T1474	5255-5264	VBN	denotes	decreased
T1476	5265-5271	NN	denotes	nestin
T1475	5272-5282	NN	denotes	expression
T1477	5283-5284	-LRB-	denotes	(
T1479	5284-5288	NNS	denotes	data
T1480	5289-5292	RB	denotes	not
T1478	5293-5298	VBN	denotes	shown
T1481	5298-5299	-RRB-	denotes	)
T1482	5300-5303	CC	denotes	and
T1483	5304-5313	VBN	denotes	increased
T1485	5314-5318	NN	denotes	MAP2
T1486	5319-5322	CC	denotes	and
T1487	5323-5327	NN	denotes	NeuN
T1484	5328-5338	NN	denotes	expression
T1488	5339-5340	-LRB-	denotes	(
T1490	5340-5344	NN	denotes	Fig.
T1491	5345-5347	NN	denotes	1c
T1492	5347-5348	,	denotes	,
T1489	5348-5350	NN	denotes	1d
T1493	5350-5351	-RRB-	denotes	)
T1494	5351-5353	,	denotes	,
T1495	5353-5356	CC	denotes	but
T1496	5357-5360	RB	denotes	not
T1497	5361-5371	NN	denotes	expression
T1498	5372-5374	IN	denotes	of
T1499	5375-5378	DT	denotes	the
T1501	5379-5387	NNP	denotes	Purkinje
T1500	5388-5394	NN	denotes	marker
T1502	5394-5396	,	denotes	,
T1503	5396-5405	NN	denotes	calbindin
T1504	5406-5407	-LRB-	denotes	(
T1506	5407-5411	NN	denotes	Fig.
T1505	5412-5414	NN	denotes	1e
T1507	5414-5415	-RRB-	denotes	)
T1508	5415-5416	.	denotes	.
T7982	5427-5435	JJ	denotes	Neuronal
T7983	5436-5442	NN	denotes	marker
T7984	5443-5453	NN	denotes	expression
T7986	5454-5456	IN	denotes	in
T7987	5457-5463	NN	denotes	CbCln3
T7989	5463-5464	SYM	denotes	+
T7990	5464-5465	HYPH	denotes	/
T7991	5465-5466	SYM	denotes	+
T7988	5467-5472	NNS	denotes	cells
T7992	5473-5489	NN	denotes	Characterization
T7993	5490-5492	IN	denotes	of
T7994	5493-5499	NN	denotes	CbCln3
T7996	5499-5500	SYM	denotes	+
T7997	5500-5501	HYPH	denotes	/
T7998	5501-5502	SYM	denotes	+
T7995	5503-5508	NNS	denotes	cells
T7999	5509-5511	IN	denotes	by
T8000	5512-5530	NN	denotes	immunofluorescence
T8001	5531-5535	IN	denotes	with
T8002	5536-5542	NN	denotes	marker
T8003	5543-5553	NNS	denotes	antibodies
T8004	5554-5556	VBZ	denotes	is
T7985	5557-5562	VBN	denotes	shown
T8005	5562-5563	.	denotes	.
T8006	5563-5690	sentence	denotes	CbCln3+/+ precursors exhibit nestin expression (a) but not GFAP expression (b), consistent with a neuronal precursor identity.
T8007	5564-5570	NN	denotes	CbCln3
T8009	5570-5571	SYM	denotes	+
T8010	5571-5572	HYPH	denotes	/
T8011	5572-5573	SYM	denotes	+
T8008	5574-5584	NNS	denotes	precursors
T8012	5585-5592	VBP	denotes	exhibit
T8013	5593-5599	NN	denotes	nestin
T8014	5600-5610	NN	denotes	expression
T8015	5611-5612	-LRB-	denotes	(
T8016	5612-5613	NN	denotes	a
T8017	5613-5614	-RRB-	denotes	)
T8018	5615-5618	CC	denotes	but
T8019	5619-5622	RB	denotes	not
T8020	5623-5627	NN	denotes	GFAP
T8021	5628-5638	NN	denotes	expression
T8022	5639-5640	-LRB-	denotes	(
T8023	5640-5641	NN	denotes	b
T8024	5641-5642	-RRB-	denotes	)
T8025	5642-5644	,	denotes	,
T8026	5644-5654	JJ	denotes	consistent
T8027	5655-5659	IN	denotes	with
T8028	5660-5661	DT	denotes	a
T8030	5662-5670	JJ	denotes	neuronal
T8031	5671-5680	NN	denotes	precursor
T8029	5681-5689	NN	denotes	identity
T8032	5689-5690	.	denotes	.
T8033	5690-5908	sentence	denotes	Upon stimulation with a differentiation cocktail (see Methods), CbCln3+/+ cells achieved neuron-like morphology, with rounded cell bodies and extension of processes, and MAP2 (c) and NeuN (d) expression was increased.
T8034	5691-5695	IN	denotes	Upon
T8036	5696-5707	NN	denotes	stimulation
T8037	5708-5712	IN	denotes	with
T8038	5713-5714	DT	denotes	a
T8040	5715-5730	NN	denotes	differentiation
T8039	5731-5739	NN	denotes	cocktail
T8041	5740-5741	-LRB-	denotes	(
T8042	5741-5744	VB	denotes	see
T8043	5745-5752	NNS	denotes	Methods
T8044	5752-5753	-RRB-	denotes	)
T8045	5753-5755	,	denotes	,
T8046	5755-5761	NN	denotes	CbCln3
T8048	5761-5762	SYM	denotes	+
T8049	5762-5763	HYPH	denotes	/
T8050	5763-5764	SYM	denotes	+
T8047	5765-5770	NNS	denotes	cells
T8035	5771-5779	VBD	denotes	achieved
T8051	5780-5786	NN	denotes	neuron
T8053	5786-5787	HYPH	denotes	-
T8052	5787-5791	JJ	denotes	like
T8054	5792-5802	NN	denotes	morphology
T8055	5802-5804	,	denotes	,
T8056	5804-5808	IN	denotes	with
T8057	5809-5816	JJ	denotes	rounded
T8059	5817-5821	NN	denotes	cell
T8058	5822-5828	NNS	denotes	bodies
T8060	5829-5832	CC	denotes	and
T8061	5833-5842	NN	denotes	extension
T8062	5843-5845	IN	denotes	of
T8063	5846-5855	NNS	denotes	processes
T8064	5855-5857	,	denotes	,
T8065	5857-5860	CC	denotes	and
T8066	5861-5865	NN	denotes	MAP2
T8068	5866-5867	-LRB-	denotes	(
T8069	5867-5868	NN	denotes	c
T8070	5868-5869	-RRB-	denotes	)
T8071	5870-5873	CC	denotes	and
T8072	5874-5878	NN	denotes	NeuN
T8073	5879-5880	-LRB-	denotes	(
T8074	5880-5881	NN	denotes	d
T8075	5881-5882	-RRB-	denotes	)
T8067	5883-5893	NN	denotes	expression
T8077	5894-5897	VBD	denotes	was
T8076	5898-5907	VBN	denotes	increased
T8078	5907-5908	.	denotes	.
T8079	5908-5983	sentence	denotes	CbCln3+/+ cells are negative for the Purkinje neuron marker calbindin (e).
T8080	5909-5915	NN	denotes	CbCln3
T8082	5915-5916	SYM	denotes	+
T8083	5916-5917	HYPH	denotes	/
T8084	5917-5918	SYM	denotes	+
T8081	5919-5924	NNS	denotes	cells
T8085	5925-5928	VBP	denotes	are
T8086	5929-5937	JJ	denotes	negative
T8087	5938-5941	IN	denotes	for
T8088	5942-5945	DT	denotes	the
T8090	5946-5954	NNP	denotes	Purkinje
T8091	5955-5961	NN	denotes	neuron
T8089	5962-5968	NN	denotes	marker
T8092	5969-5978	NN	denotes	calbindin
T8093	5979-5980	-LRB-	denotes	(
T8094	5980-5981	NN	denotes	e
T8095	5981-5982	-RRB-	denotes	)
T8096	5982-5983	.	denotes	.
T8097	5983-6088	sentence	denotes	CbCln3+/Δex7/8 and CbCln3Δex7/8/Δex7/8 cell lines exhibited identical marker immunofluorescence results.
T8098	5984-5990	NN	denotes	CbCln3
T8100	5990-5991	SYM	denotes	+
T8101	5991-5992	HYPH	denotes	/
T8099	5992-5996	NN	denotes	Δex7
T8103	5996-5997	HYPH	denotes	/
T8104	5997-5998	CD	denotes	8
T8105	5999-6002	CC	denotes	and
T8106	6003-6013	NN	denotes	CbCln3Δex7
T8108	6013-6014	HYPH	denotes	/
T8109	6014-6015	CD	denotes	8
T8110	6015-6016	HYPH	denotes	/
T8107	6016-6020	NN	denotes	Δex7
T8111	6020-6021	HYPH	denotes	/
T8112	6021-6022	CD	denotes	8
T8113	6023-6027	NN	denotes	cell
T8102	6028-6033	NNS	denotes	lines
T8114	6034-6043	VBD	denotes	exhibited
T8115	6044-6053	JJ	denotes	identical
T8117	6054-6060	NN	denotes	marker
T8118	6061-6079	NN	denotes	immunofluorescence
T8116	6080-6087	NNS	denotes	results
T8119	6087-6088	.	denotes	.
T8120	6088-6143	sentence	denotes	a, b) 20 × magnification; c, d, e) 40 × magnification.
T8121	6089-6090	LS	denotes	a
T8123	6090-6092	,	denotes	,
T8124	6092-6093	LS	denotes	b
T8125	6093-6094	-RRB-	denotes	)
T8126	6095-6097	CD	denotes	20
T8127	6098-6099	SYM	denotes	×
T8122	6100-6113	NN	denotes	magnification
T8128	6113-6114	:	denotes	;
T8129	6115-6116	LS	denotes	c
T8131	6116-6118	,	denotes	,
T8132	6118-6119	LS	denotes	d
T8133	6119-6121	,	denotes	,
T8134	6121-6122	LS	denotes	e
T8135	6122-6123	-RRB-	denotes	)
T8136	6124-6126	CD	denotes	40
T8137	6127-6128	SYM	denotes	×
T8130	6129-6142	NN	denotes	magnification
T8138	6142-6143	.	denotes	.
T1775	6145-6155	JJ	denotes	Homozygous
T1777	6156-6166	NN	denotes	CbCln3Δex7
T1778	6166-6167	HYPH	denotes	/
T1779	6167-6168	CD	denotes	8
T1776	6169-6174	NNS	denotes	cells
T1780	6175-6182	VBP	denotes	express
T1781	6183-6189	NN	denotes	mutant
T1782	6190-6198	NN	denotes	battenin
T1783	6199-6202	CC	denotes	and
T1784	6203-6210	VBP	denotes	display
T1785	6211-6215	NN	denotes	JNCL
T1787	6215-6216	HYPH	denotes	-
T1786	6216-6220	JJ	denotes	like
T1788	6221-6230	NN	denotes	pathology
T1789	6230-6312	sentence	denotes	Homozygous Cb Cln3Δex7/8 cells were first examined for JNCL-like characteristics.
T1790	6231-6241	JJ	denotes	Homozygous
T1792	6242-6244	NN	denotes	Cb
T1793	6245-6253	NN	denotes	Cln3Δex7
T1794	6253-6254	HYPH	denotes	/
T1795	6254-6255	CD	denotes	8
T1791	6256-6261	NNS	denotes	cells
T1797	6262-6266	VBD	denotes	were
T1798	6267-6272	RB	denotes	first
T1796	6273-6281	VBN	denotes	examined
T1799	6282-6285	IN	denotes	for
T1800	6286-6290	NN	denotes	JNCL
T1802	6290-6291	HYPH	denotes	-
T1801	6291-6295	JJ	denotes	like
T1803	6296-6311	NNS	denotes	characteristics
T1804	6311-6312	.	denotes	.
T1805	6312-6494	sentence	denotes	Homozygous Cln3Δex7/8 knock-in mice express multiple Cln3 mRNA splice variants and mutant battenin protein that is detectable by batp1 antibody recognizing C-terminal epitopes [12].
T1806	6313-6323	JJ	denotes	Homozygous
T1808	6324-6332	NN	denotes	Cln3Δex7
T1809	6332-6333	HYPH	denotes	/
T1810	6333-6334	CD	denotes	8
T1811	6335-6340	VB	denotes	knock
T1812	6340-6341	HYPH	denotes	-
T1813	6341-6343	RP	denotes	in
T1807	6344-6348	NNS	denotes	mice
T1814	6349-6356	VBP	denotes	express
T1815	6357-6365	JJ	denotes	multiple
T1817	6366-6370	NN	denotes	Cln3
T1819	6371-6375	NN	denotes	mRNA
T1818	6376-6382	NN	denotes	splice
T1816	6383-6391	NNS	denotes	variants
T1820	6392-6395	CC	denotes	and
T1821	6396-6402	NN	denotes	mutant
T1823	6403-6411	NN	denotes	battenin
T1822	6412-6419	NN	denotes	protein
T1824	6420-6424	WDT	denotes	that
T1825	6425-6427	VBZ	denotes	is
T1826	6428-6438	JJ	denotes	detectable
T1827	6439-6441	IN	denotes	by
T1828	6442-6447	NN	denotes	batp1
T1829	6448-6456	NN	denotes	antibody
T1830	6457-6468	VBG	denotes	recognizing
T1832	6469-6470	NN	denotes	C
T1834	6470-6471	HYPH	denotes	-
T1833	6471-6479	JJ	denotes	terminal
T1831	6480-6488	NNS	denotes	epitopes
T1835	6489-6490	-LRB-	denotes	[
T1836	6490-6492	CD	denotes	12
T1837	6492-6493	-RRB-	denotes	]
T1838	6493-6494	.	denotes	.
T1839	6494-6616	sentence	denotes	To assess this molecular phenotype in CbCln3Δex7/8 cells, RT-PCR and anti-battenin (batp1) immunostaining were performed.
T1840	6495-6497	TO	denotes	To
T1841	6498-6504	VB	denotes	assess
T1843	6505-6509	DT	denotes	this
T1845	6510-6519	JJ	denotes	molecular
T1844	6520-6529	NN	denotes	phenotype
T1846	6530-6532	IN	denotes	in
T1847	6533-6543	NN	denotes	CbCln3Δex7
T1849	6543-6544	HYPH	denotes	/
T1850	6544-6545	CD	denotes	8
T1848	6546-6551	NNS	denotes	cells
T1851	6551-6553	,	denotes	,
T1852	6553-6555	NN	denotes	RT
T1854	6555-6556	HYPH	denotes	-
T1853	6556-6559	NN	denotes	PCR
T1855	6560-6563	CC	denotes	and
T1856	6564-6577	JJ	denotes	anti-battenin
T1858	6578-6579	-LRB-	denotes	(
T1859	6579-6584	NN	denotes	batp1
T1860	6584-6585	-RRB-	denotes	)
T1857	6586-6600	NN	denotes	immunostaining
T1861	6601-6605	VBD	denotes	were
T1842	6606-6615	VBN	denotes	performed
T1862	6615-6616	.	denotes	.
T1863	6616-6823	sentence	denotes	As shown in Figure 2, Cln3 mRNA isoforms in wild-type and homozygous cells were similar to those observed in total RNA isolated from wild-type or homozygous Cln3Δex7/8 knock-in brain, respectively (Fig. 2).
T1864	6617-6619	IN	denotes	As
T1865	6620-6625	VBN	denotes	shown
T1867	6626-6628	IN	denotes	in
T1868	6629-6635	NN	denotes	Figure
T1869	6636-6637	CD	denotes	2
T1870	6637-6639	,	denotes	,
T1871	6639-6643	NN	denotes	Cln3
T1873	6644-6648	NN	denotes	mRNA
T1872	6649-6657	NNS	denotes	isoforms
T1874	6658-6660	IN	denotes	in
T1875	6661-6665	JJ	denotes	wild
T1877	6665-6666	HYPH	denotes	-
T1876	6666-6670	NN	denotes	type
T1879	6671-6674	CC	denotes	and
T1880	6675-6685	JJ	denotes	homozygous
T1878	6686-6691	NNS	denotes	cells
T1866	6692-6696	VBD	denotes	were
T1881	6697-6704	JJ	denotes	similar
T1882	6705-6707	IN	denotes	to
T1883	6708-6713	DT	denotes	those
T1884	6714-6722	VBN	denotes	observed
T1885	6723-6725	IN	denotes	in
T1886	6726-6731	JJ	denotes	total
T1887	6732-6735	NN	denotes	RNA
T1888	6736-6744	VBN	denotes	isolated
T1889	6745-6749	IN	denotes	from
T1890	6750-6754	JJ	denotes	wild
T1892	6754-6755	HYPH	denotes	-
T1891	6755-6759	NN	denotes	type
T1894	6760-6762	CC	denotes	or
T1895	6763-6773	JJ	denotes	homozygous
T1896	6774-6782	NN	denotes	Cln3Δex7
T1897	6782-6783	HYPH	denotes	/
T1898	6783-6784	CD	denotes	8
T1899	6785-6790	VB	denotes	knock
T1900	6790-6791	HYPH	denotes	-
T1901	6791-6793	RP	denotes	in
T1893	6794-6799	NN	denotes	brain
T1902	6799-6801	,	denotes	,
T1903	6801-6813	RB	denotes	respectively
T1904	6814-6815	-LRB-	denotes	(
T1905	6815-6819	NN	denotes	Fig.
T1906	6820-6821	CD	denotes	2
T1907	6821-6822	-RRB-	denotes	)
T1908	6822-6823	.	denotes	.
T1909	6823-7025	sentence	denotes	In addition, batp1 immunostaining detected mutant battenin product in homozygous CbCln3Δex7/8 cells, in a similar albeit reduced cytoplasmic, vesicular staining pattern as that seen in wild-type cells.
T1910	6824-6826	IN	denotes	In
T1912	6827-6835	NN	denotes	addition
T1913	6835-6837	,	denotes	,
T1914	6837-6842	NN	denotes	batp1
T1915	6843-6857	NN	denotes	immunostaining
T1911	6858-6866	VBD	denotes	detected
T1916	6867-6873	NN	denotes	mutant
T1918	6874-6882	NN	denotes	battenin
T1917	6883-6890	NN	denotes	product
T1919	6891-6893	IN	denotes	in
T1920	6894-6904	JJ	denotes	homozygous
T1922	6905-6915	NN	denotes	CbCln3Δex7
T1923	6915-6916	HYPH	denotes	/
T1924	6916-6917	CD	denotes	8
T1921	6918-6923	NNS	denotes	cells
T1925	6923-6925	,	denotes	,
T1926	6925-6927	IN	denotes	in
T1927	6928-6929	DT	denotes	a
T1929	6930-6937	JJ	denotes	similar
T1930	6938-6944	IN	denotes	albeit
T1931	6945-6952	VBN	denotes	reduced
T1932	6953-6964	JJ	denotes	cytoplasmic
T1933	6964-6966	,	denotes	,
T1934	6966-6975	JJ	denotes	vesicular
T1935	6976-6984	NN	denotes	staining
T1928	6985-6992	NN	denotes	pattern
T1936	6993-6995	IN	denotes	as
T1937	6996-7000	DT	denotes	that
T1938	7001-7005	VBN	denotes	seen
T1939	7006-7008	IN	denotes	in
T1940	7009-7013	JJ	denotes	wild
T1942	7013-7014	HYPH	denotes	-
T1941	7014-7018	NN	denotes	type
T1943	7019-7024	NNS	denotes	cells
T1944	7024-7025	.	denotes	.
T1945	7025-7211	sentence	denotes	Batp1 signal exhibited some overlap with the lysosomal marker, Lamp1, but had more significant overlap with early endosome antigen 1 (EEA1) and the late endosomal marker, Rab7 (Fig. 3).
T1946	7026-7031	NN	denotes	Batp1
T1947	7032-7038	NN	denotes	signal
T1948	7039-7048	VBD	denotes	exhibited
T1949	7049-7053	DT	denotes	some
T1950	7054-7061	NN	denotes	overlap
T1951	7062-7066	IN	denotes	with
T1952	7067-7070	DT	denotes	the
T1954	7071-7080	JJ	denotes	lysosomal
T1953	7081-7087	NN	denotes	marker
T1955	7087-7089	,	denotes	,
T1956	7089-7094	NN	denotes	Lamp1
T1957	7094-7096	,	denotes	,
T1958	7096-7099	CC	denotes	but
T1959	7100-7103	VBD	denotes	had
T1960	7104-7108	RBR	denotes	more
T1961	7109-7120	JJ	denotes	significant
T1962	7121-7128	NN	denotes	overlap
T1963	7129-7133	IN	denotes	with
T1964	7134-7139	JJ	denotes	early
T1966	7140-7148	NN	denotes	endosome
T1965	7149-7156	NN	denotes	antigen
T1967	7157-7158	CD	denotes	1
T1968	7159-7160	-LRB-	denotes	(
T1969	7160-7164	NN	denotes	EEA1
T1970	7164-7165	-RRB-	denotes	)
T1971	7166-7169	CC	denotes	and
T1972	7170-7173	DT	denotes	the
T1974	7174-7178	JJ	denotes	late
T1975	7179-7188	JJ	denotes	endosomal
T1973	7189-7195	NN	denotes	marker
T1976	7195-7197	,	denotes	,
T1977	7197-7201	NN	denotes	Rab7
T1978	7202-7203	-LRB-	denotes	(
T1979	7203-7207	NN	denotes	Fig.
T1980	7208-7209	CD	denotes	3
T1981	7209-7210	-RRB-	denotes	)
T1982	7210-7211	.	denotes	.
T1983	7211-7339	sentence	denotes	Only limited overlap was observed with recycling endosomes, as determined by transferrin receptor co-staining (data not shown).
T1984	7212-7216	RB	denotes	Only
T1986	7217-7224	VBN	denotes	limited
T1985	7225-7232	NN	denotes	overlap
T1988	7233-7236	VBD	denotes	was
T1987	7237-7245	VBN	denotes	observed
T1989	7246-7250	IN	denotes	with
T1990	7251-7260	VBG	denotes	recycling
T1991	7261-7270	NNS	denotes	endosomes
T1992	7270-7272	,	denotes	,
T1993	7272-7274	IN	denotes	as
T1994	7275-7285	VBN	denotes	determined
T1995	7286-7288	IN	denotes	by
T1996	7289-7300	NN	denotes	transferrin
T1997	7301-7309	NN	denotes	receptor
T1998	7310-7321	NN	denotes	co-staining
T1999	7322-7323	-LRB-	denotes	(
T2001	7323-7327	NNS	denotes	data
T2002	7328-7331	RB	denotes	not
T2000	7332-7337	VBN	denotes	shown
T2003	7337-7338	-RRB-	denotes	)
T2004	7338-7339	.	denotes	.
T2005	7339-7594	sentence	denotes	Intriguingly, Lamp1 and EEA1 immunocytochemical distribution were altered in homozygous CbCln3Δex7/8 cells, with less perinuclear clustering than in wild-type cells, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells (Fig. 3).
T2006	7340-7352	RB	denotes	Intriguingly
T2008	7352-7354	,	denotes	,
T2009	7354-7359	NN	denotes	Lamp1
T2011	7360-7363	CC	denotes	and
T2012	7364-7368	NN	denotes	EEA1
T2013	7369-7387	JJ	denotes	immunocytochemical
T2010	7388-7400	NN	denotes	distribution
T2014	7401-7405	VBD	denotes	were
T2007	7406-7413	VBN	denotes	altered
T2015	7414-7416	IN	denotes	in
T2016	7417-7427	JJ	denotes	homozygous
T2018	7428-7438	NN	denotes	CbCln3Δex7
T2019	7438-7439	HYPH	denotes	/
T2020	7439-7440	CD	denotes	8
T2017	7441-7446	NNS	denotes	cells
T2021	7446-7448	,	denotes	,
T2022	7448-7452	IN	denotes	with
T2023	7453-7457	JJR	denotes	less
T2025	7458-7469	JJ	denotes	perinuclear
T2024	7470-7480	NN	denotes	clustering
T2026	7481-7485	IN	denotes	than
T2027	7486-7488	IN	denotes	in
T2028	7489-7493	JJ	denotes	wild
T2030	7493-7494	HYPH	denotes	-
T2029	7494-7498	NN	denotes	type
T2031	7499-7504	NNS	denotes	cells
T2032	7504-7506	,	denotes	,
T2033	7506-7509	CC	denotes	and
T2034	7510-7514	NN	denotes	Rab7
T2035	7515-7523	NN	denotes	staining
T2036	7524-7527	VBD	denotes	was
T2037	7528-7538	RB	denotes	frequently
T2038	7539-7543	RBR	denotes	less
T2039	7544-7551	JJ	denotes	intense
T2040	7552-7554	IN	denotes	in
T2041	7555-7565	JJ	denotes	homozygous
T2043	7566-7576	NN	denotes	CbCln3Δex7
T2044	7576-7577	HYPH	denotes	/
T2045	7577-7578	CD	denotes	8
T2042	7579-7584	NNS	denotes	cells
T2046	7585-7586	-LRB-	denotes	(
T2047	7586-7590	NN	denotes	Fig.
T2048	7591-7592	CD	denotes	3
T2049	7592-7593	-RRB-	denotes	)
T2050	7593-7594	.	denotes	.
T2051	7594-7801	sentence	denotes	Heterozygous CbCln3Δex7/8 cells contained a mixture of Cln3 mRNA products from both the wild-type allele and the mutant allele, and batp1 signal was similar to that seen in wild-type cells (data not shown).
T2052	7595-7607	JJ	denotes	Heterozygous
T2054	7608-7618	NN	denotes	CbCln3Δex7
T2055	7618-7619	HYPH	denotes	/
T2056	7619-7620	CD	denotes	8
T2053	7621-7626	NNS	denotes	cells
T2057	7627-7636	VBD	denotes	contained
T2058	7637-7638	DT	denotes	a
T2059	7639-7646	NN	denotes	mixture
T2060	7647-7649	IN	denotes	of
T2061	7650-7654	NN	denotes	Cln3
T2063	7655-7659	NN	denotes	mRNA
T2062	7660-7668	NNS	denotes	products
T2064	7669-7673	IN	denotes	from
T2065	7674-7678	CC	denotes	both
T2067	7679-7682	DT	denotes	the
T2068	7683-7687	JJ	denotes	wild
T2070	7687-7688	HYPH	denotes	-
T2069	7688-7692	NN	denotes	type
T2066	7693-7699	NN	denotes	allele
T2071	7700-7703	CC	denotes	and
T2072	7704-7707	DT	denotes	the
T2074	7708-7714	NN	denotes	mutant
T2073	7715-7721	NN	denotes	allele
T2075	7721-7723	,	denotes	,
T2076	7723-7726	CC	denotes	and
T2077	7727-7732	NN	denotes	batp1
T2078	7733-7739	NN	denotes	signal
T2079	7740-7743	VBD	denotes	was
T2080	7744-7751	JJ	denotes	similar
T2081	7752-7754	IN	denotes	to
T2082	7755-7759	DT	denotes	that
T2083	7760-7764	VBN	denotes	seen
T2084	7765-7767	IN	denotes	in
T2085	7768-7772	JJ	denotes	wild
T2087	7772-7773	HYPH	denotes	-
T2086	7773-7777	NN	denotes	type
T2088	7778-7783	NNS	denotes	cells
T2089	7784-7785	-LRB-	denotes	(
T2091	7785-7789	NNS	denotes	data
T2092	7790-7793	RB	denotes	not
T2090	7794-7799	VBN	denotes	shown
T2093	7799-7800	-RRB-	denotes	)
T2094	7800-7801	.	denotes	.
T2095	7801-9594	sentence	denotes	Figure 2 RT-PCR of Cln3 mRNA in wild-type and homozygous CbCln3Δex7/8 cells Cln3 Exon1-forward, Exon 15-reverse RT-PCR products are shown, from total wild-type (+/+) or homozygous mutant (Δex7/8/Δex7/8) brain and cell line RNA. Brain and cell line RT-PCR reaction products had identical band patterns on ethidium-bromide stained agarose gels. Wild-type RT-PCR product was a single ~1.6 kb band and mutant products were ~1.6, ~1.5, ~1.4, ~1.35, and ~1.3 kb, representing multiple mutant splice variants. Figure 3 Battenin and lysosomal and endosomal marker co-staining in wild-type and homozygous CbCln3Δex7/8 cerebellar precursor cells Batp1 immunostaining of wild-type (CbCln3+/+) and homozygous mutant (CbCln3Δex7/8/Δex7/8) cerebellar precursor cells is shown, with co-staining for lysosomes (Lamp 1), early endosomes (EEA1), and late endosomes (Rab7). Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge). The degree of Batp1 overlap is greatest with Rab7. Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen. Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels. Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells. Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification. During sub-confluent growth conditions, neither wild-type nor homozygous CbCln3Δex7/8 cells displayed autofluorescence or subunit c inclusion formation (data not shown).
T8215	7812-7814	NN	denotes	RT
T8217	7814-7815	HYPH	denotes	-
T8216	7815-7818	NN	denotes	PCR
T8219	7819-7821	IN	denotes	of
T8220	7822-7826	NN	denotes	Cln3
T8221	7827-7831	NN	denotes	mRNA
T8222	7832-7834	IN	denotes	in
T8223	7835-7839	JJ	denotes	wild
T8225	7839-7840	HYPH	denotes	-
T8224	7840-7844	NN	denotes	type
T8227	7845-7848	CC	denotes	and
T8228	7849-7859	JJ	denotes	homozygous
T8229	7860-7870	NN	denotes	CbCln3Δex7
T8230	7870-7871	HYPH	denotes	/
T8231	7871-7872	CD	denotes	8
T8226	7873-7878	NNS	denotes	cells
T8232	7879-7883	NN	denotes	Cln3
T8234	7884-7889	NN	denotes	Exon1
T8236	7889-7890	HYPH	denotes	-
T8235	7890-7897	JJ	denotes	forward
T8238	7897-7899	,	denotes	,
T8239	7899-7903	NN	denotes	Exon
T8240	7904-7906	CD	denotes	15
T8241	7906-7907	HYPH	denotes	-
T8237	7907-7914	JJ	denotes	reverse
T8242	7915-7917	NN	denotes	RT
T8244	7917-7918	HYPH	denotes	-
T8243	7918-7921	NN	denotes	PCR
T8233	7922-7930	NNS	denotes	products
T8245	7931-7934	VBP	denotes	are
T8218	7935-7940	VBN	denotes	shown
T8246	7940-7942	,	denotes	,
T8247	7942-7946	IN	denotes	from
T8248	7947-7952	JJ	denotes	total
T8250	7953-7957	JJ	denotes	wild
T8252	7957-7958	HYPH	denotes	-
T8251	7958-7962	NN	denotes	type
T8253	7963-7964	-LRB-	denotes	(
T8254	7964-7965	SYM	denotes	+
T8256	7965-7966	HYPH	denotes	/
T8255	7966-7967	SYM	denotes	+
T8257	7967-7968	-RRB-	denotes	)
T8258	7969-7971	CC	denotes	or
T8259	7972-7982	JJ	denotes	homozygous
T8260	7983-7989	NN	denotes	mutant
T8261	7990-7991	-LRB-	denotes	(
T8262	7991-7995	NN	denotes	Δex7
T8263	7995-7996	HYPH	denotes	/
T8264	7996-7997	CD	denotes	8
T8265	7997-7998	HYPH	denotes	/
T8266	7998-8002	NN	denotes	Δex7
T8267	8002-8003	HYPH	denotes	/
T8268	8003-8004	CD	denotes	8
T8269	8004-8005	-RRB-	denotes	)
T8270	8006-8011	NN	denotes	brain
T8271	8012-8015	CC	denotes	and
T8272	8016-8020	NN	denotes	cell
T8273	8021-8025	NN	denotes	line
T8249	8026-8029	NN	denotes	RNA
T8274	8029-8030	.	denotes	.
T8275	8030-8145	sentence	denotes	Brain and cell line RT-PCR reaction products had identical band patterns on ethidium-bromide stained agarose gels.
T8276	8031-8036	NN	denotes	Brain
T8278	8037-8040	CC	denotes	and
T8279	8041-8045	NN	denotes	cell
T8280	8046-8050	NN	denotes	line
T8281	8051-8053	NN	denotes	RT
T8283	8053-8054	HYPH	denotes	-
T8282	8054-8057	NN	denotes	PCR
T8284	8058-8066	NN	denotes	reaction
T8277	8067-8075	NNS	denotes	products
T8285	8076-8079	VBD	denotes	had
T8286	8080-8089	JJ	denotes	identical
T8288	8090-8094	NN	denotes	band
T8287	8095-8103	NNS	denotes	patterns
T8289	8104-8106	IN	denotes	on
T8290	8107-8115	NN	denotes	ethidium
T8292	8115-8116	HYPH	denotes	-
T8291	8116-8123	NN	denotes	bromide
T8293	8124-8131	VBN	denotes	stained
T8295	8132-8139	NN	denotes	agarose
T8294	8140-8144	NNS	denotes	gels
T8296	8144-8145	.	denotes	.
T8297	8145-8305	sentence	denotes	Wild-type RT-PCR product was a single ~1.6 kb band and mutant products were ~1.6, ~1.5, ~1.4, ~1.35, and ~1.3 kb, representing multiple mutant splice variants.
T8298	8146-8150	JJ	denotes	Wild
T8300	8150-8151	HYPH	denotes	-
T8299	8151-8155	NN	denotes	type
T8302	8156-8158	NN	denotes	RT
T8304	8158-8159	HYPH	denotes	-
T8303	8159-8162	NN	denotes	PCR
T8301	8163-8170	NN	denotes	product
T8305	8171-8174	VBD	denotes	was
T8306	8175-8176	DT	denotes	a
T8308	8177-8183	JJ	denotes	single
T8309	8184-8185	SYM	denotes	~
T8310	8185-8188	CD	denotes	1.6
T8311	8189-8191	NN	denotes	kb
T8307	8192-8196	NN	denotes	band
T8312	8197-8200	CC	denotes	and
T8313	8201-8207	NN	denotes	mutant
T8314	8208-8216	NNS	denotes	products
T8315	8217-8221	VBD	denotes	were
T8316	8222-8223	SYM	denotes	~
T8317	8223-8226	CD	denotes	1.6
T8319	8226-8228	,	denotes	,
T8320	8228-8229	SYM	denotes	~
T8321	8229-8232	CD	denotes	1.5
T8322	8232-8234	,	denotes	,
T8323	8234-8235	SYM	denotes	~
T8324	8235-8238	CD	denotes	1.4
T8325	8238-8240	,	denotes	,
T8326	8240-8241	SYM	denotes	~
T8327	8241-8245	CD	denotes	1.35
T8328	8245-8247	,	denotes	,
T8329	8247-8250	CC	denotes	and
T8330	8251-8252	SYM	denotes	~
T8331	8252-8255	NN	denotes	1.3
T8318	8256-8258	NN	denotes	kb
T8332	8258-8260	,	denotes	,
T8333	8260-8272	VBG	denotes	representing
T8334	8273-8281	JJ	denotes	multiple
T8336	8282-8288	NN	denotes	mutant
T8337	8289-8295	NN	denotes	splice
T8335	8296-8304	NNS	denotes	variants
T8338	8304-8305	.	denotes	.
T8445	8316-8324	NN	denotes	Battenin
T8447	8325-8328	CC	denotes	and
T8448	8329-8338	JJ	denotes	lysosomal
T8449	8339-8342	CC	denotes	and
T8450	8343-8352	JJ	denotes	endosomal
T8446	8353-8359	NN	denotes	marker
T8451	8360-8371	NN	denotes	co-staining
T8453	8372-8374	IN	denotes	in
T8454	8375-8379	JJ	denotes	wild
T8456	8379-8380	HYPH	denotes	-
T8455	8380-8384	NN	denotes	type
T8458	8385-8388	CC	denotes	and
T8459	8389-8399	JJ	denotes	homozygous
T8460	8400-8410	NN	denotes	CbCln3Δex7
T8462	8410-8411	HYPH	denotes	/
T8463	8411-8412	CD	denotes	8
T8464	8413-8423	JJ	denotes	cerebellar
T8461	8424-8433	NN	denotes	precursor
T8457	8434-8439	NNS	denotes	cells
T8465	8440-8445	NN	denotes	Batp1
T8466	8446-8460	NN	denotes	immunostaining
T8467	8461-8463	IN	denotes	of
T8468	8464-8468	JJ	denotes	wild
T8470	8468-8469	HYPH	denotes	-
T8469	8469-8473	NN	denotes	type
T8471	8474-8475	-LRB-	denotes	(
T8472	8475-8481	NN	denotes	CbCln3
T8473	8481-8482	SYM	denotes	+
T8474	8482-8483	HYPH	denotes	/
T8475	8483-8484	SYM	denotes	+
T8476	8484-8485	-RRB-	denotes	)
T8477	8486-8489	CC	denotes	and
T8478	8490-8500	JJ	denotes	homozygous
T8479	8501-8507	NN	denotes	mutant
T8481	8508-8509	-LRB-	denotes	(
T8482	8509-8519	NN	denotes	CbCln3Δex7
T8484	8519-8520	HYPH	denotes	/
T8485	8520-8521	CD	denotes	8
T8486	8521-8522	HYPH	denotes	/
T8483	8522-8526	NN	denotes	Δex7
T8487	8526-8527	HYPH	denotes	/
T8488	8527-8528	CD	denotes	8
T8489	8528-8529	-RRB-	denotes	)
T8490	8530-8540	JJ	denotes	cerebellar
T8491	8541-8550	NN	denotes	precursor
T8480	8551-8556	NNS	denotes	cells
T8492	8557-8559	VBZ	denotes	is
T8452	8560-8565	VBN	denotes	shown
T8493	8565-8567	,	denotes	,
T8494	8567-8571	IN	denotes	with
T8495	8572-8583	NN	denotes	co-staining
T8496	8584-8587	IN	denotes	for
T8497	8588-8597	NNS	denotes	lysosomes
T8498	8598-8599	-LRB-	denotes	(
T8499	8599-8603	NN	denotes	Lamp
T8500	8604-8605	CD	denotes	1
T8501	8605-8606	-RRB-	denotes	)
T8502	8606-8608	,	denotes	,
T8503	8608-8613	JJ	denotes	early
T8504	8614-8623	NNS	denotes	endosomes
T8505	8624-8625	-LRB-	denotes	(
T8506	8625-8629	NN	denotes	EEA1
T8507	8629-8630	-RRB-	denotes	)
T8508	8630-8632	,	denotes	,
T8509	8632-8635	CC	denotes	and
T8510	8636-8640	JJ	denotes	late
T8511	8641-8650	NNS	denotes	endosomes
T8512	8651-8652	-LRB-	denotes	(
T8513	8652-8656	NN	denotes	Rab7
T8514	8656-8657	-RRB-	denotes	)
T8515	8657-8658	.	denotes	.
T8516	8658-8829	sentence	denotes	Significant overlap of Batp1 signal (red) with EEA1 (green, middle panels) and Rab7 (green, bottom panels) can be seen as yellow when the two channels are merged (Merge).
T8517	8659-8670	JJ	denotes	Significant
T8518	8671-8678	NN	denotes	overlap
T8520	8679-8681	IN	denotes	of
T8521	8682-8687	NN	denotes	Batp1
T8522	8688-8694	NN	denotes	signal
T8523	8695-8696	-LRB-	denotes	(
T8524	8696-8699	JJ	denotes	red
T8525	8699-8700	-RRB-	denotes	)
T8526	8701-8705	IN	denotes	with
T8527	8706-8710	NN	denotes	EEA1
T8528	8711-8712	-LRB-	denotes	(
T8530	8712-8717	JJ	denotes	green
T8531	8717-8719	,	denotes	,
T8532	8719-8725	JJ	denotes	middle
T8529	8726-8732	NNS	denotes	panels
T8533	8732-8733	-RRB-	denotes	)
T8534	8734-8737	CC	denotes	and
T8535	8738-8742	NN	denotes	Rab7
T8536	8743-8744	-LRB-	denotes	(
T8538	8744-8749	JJ	denotes	green
T8539	8749-8751	,	denotes	,
T8540	8751-8757	JJ	denotes	bottom
T8537	8758-8764	NNS	denotes	panels
T8541	8764-8765	-RRB-	denotes	)
T8542	8766-8769	MD	denotes	can
T8543	8770-8772	VB	denotes	be
T8519	8773-8777	VBN	denotes	seen
T8544	8778-8780	IN	denotes	as
T8545	8781-8787	JJ	denotes	yellow
T8546	8788-8792	WRB	denotes	when
T8548	8793-8796	DT	denotes	the
T8550	8797-8800	CD	denotes	two
T8549	8801-8809	NNS	denotes	channels
T8551	8810-8813	VBP	denotes	are
T8547	8814-8820	VBN	denotes	merged
T8552	8821-8822	-LRB-	denotes	(
T8553	8822-8827	NNP	denotes	Merge
T8554	8827-8828	-RRB-	denotes	)
T8555	8828-8829	.	denotes	.
T8556	8829-8880	sentence	denotes	The degree of Batp1 overlap is greatest with Rab7.
T8557	8830-8833	DT	denotes	The
T8558	8834-8840	NN	denotes	degree
T8560	8841-8843	IN	denotes	of
T8561	8844-8849	NN	denotes	Batp1
T8562	8850-8857	NN	denotes	overlap
T8559	8858-8860	VBZ	denotes	is
T8563	8861-8869	JJS	denotes	greatest
T8564	8870-8874	IN	denotes	with
T8565	8875-8879	NN	denotes	Rab7
T8566	8879-8880	.	denotes	.
T8567	8880-8965	sentence	denotes	Only limited overlap between Batp1 (red) and Lamp 1 (green, top panels) can be seen.
T8568	8881-8885	RB	denotes	Only
T8570	8886-8893	VBN	denotes	limited
T8569	8894-8901	NN	denotes	overlap
T8572	8902-8909	IN	denotes	between
T8573	8910-8915	NN	denotes	Batp1
T8574	8916-8917	-LRB-	denotes	(
T8575	8917-8920	JJ	denotes	red
T8576	8920-8921	-RRB-	denotes	)
T8577	8922-8925	CC	denotes	and
T8578	8926-8930	NN	denotes	Lamp
T8579	8931-8932	CD	denotes	1
T8580	8933-8934	-LRB-	denotes	(
T8582	8934-8939	JJ	denotes	green
T8583	8939-8941	,	denotes	,
T8584	8941-8944	JJ	denotes	top
T8581	8945-8951	NNS	denotes	panels
T8585	8951-8952	-RRB-	denotes	)
T8586	8953-8956	MD	denotes	can
T8587	8957-8959	VB	denotes	be
T8571	8960-8964	VBN	denotes	seen
T8588	8964-8965	.	denotes	.
T8589	8965-9168	sentence	denotes	Batp1 signal in homozygous CbCln3Δex7/8 cells is significantly reduced, but significant overlap with EEA1 and Rab7, and very little Lamp 1 overlap, can be seen as yellow in the respective merged panels.
T8590	8966-8971	NN	denotes	Batp1
T8591	8972-8978	NN	denotes	signal
T8593	8979-8981	IN	denotes	in
T8594	8982-8992	JJ	denotes	homozygous
T8596	8993-9003	NN	denotes	CbCln3Δex7
T8597	9003-9004	HYPH	denotes	/
T8598	9004-9005	CD	denotes	8
T8595	9006-9011	NNS	denotes	cells
T8592	9012-9014	VBZ	denotes	is
T8599	9015-9028	RB	denotes	significantly
T8600	9029-9036	JJ	denotes	reduced
T8601	9036-9038	,	denotes	,
T8602	9038-9041	CC	denotes	but
T8603	9042-9053	JJ	denotes	significant
T8604	9054-9061	NN	denotes	overlap
T8606	9062-9066	IN	denotes	with
T8607	9067-9071	NN	denotes	EEA1
T8608	9072-9075	CC	denotes	and
T8609	9076-9080	NN	denotes	Rab7
T8610	9080-9082	,	denotes	,
T8611	9082-9085	CC	denotes	and
T8612	9086-9090	RB	denotes	very
T8613	9091-9097	JJ	denotes	little
T8615	9098-9102	NNP	denotes	Lamp
T8616	9103-9104	CD	denotes	1
T8614	9105-9112	NN	denotes	overlap
T8617	9112-9114	,	denotes	,
T8618	9114-9117	MD	denotes	can
T8619	9118-9120	VB	denotes	be
T8605	9121-9125	VBN	denotes	seen
T8620	9126-9128	IN	denotes	as
T8621	9129-9135	JJ	denotes	yellow
T8622	9136-9138	IN	denotes	in
T8623	9139-9142	DT	denotes	the
T8625	9143-9153	JJ	denotes	respective
T8626	9154-9160	JJ	denotes	merged
T8624	9161-9167	NNS	denotes	panels
T8627	9167-9168	.	denotes	.
T8628	9168-9302	sentence	denotes	Notably, Lamp 1 and EEA1 localization appear altered, and Rab7 staining was frequently less intense in homozygous CbCln3Δex7/8 cells.
T8629	9169-9176	RB	denotes	Notably
T8631	9176-9178	,	denotes	,
T8632	9178-9182	NN	denotes	Lamp
T8634	9183-9184	CD	denotes	1
T8635	9185-9188	CC	denotes	and
T8636	9189-9193	NN	denotes	EEA1
T8633	9194-9206	NN	denotes	localization
T8630	9207-9213	VBP	denotes	appear
T8637	9214-9221	JJ	denotes	altered
T8638	9221-9223	,	denotes	,
T8639	9223-9226	CC	denotes	and
T8640	9227-9231	NN	denotes	Rab7
T8641	9232-9240	NN	denotes	staining
T8642	9241-9244	VBD	denotes	was
T8643	9245-9255	RB	denotes	frequently
T8644	9256-9260	RBR	denotes	less
T8645	9261-9268	JJ	denotes	intense
T8646	9269-9271	IN	denotes	in
T8647	9272-9282	JJ	denotes	homozygous
T8649	9283-9293	NN	denotes	CbCln3Δex7
T8650	9293-9294	HYPH	denotes	/
T8651	9294-9295	CD	denotes	8
T8648	9296-9301	NNS	denotes	cells
T8652	9301-9302	.	denotes	.
T8653	9302-9404	sentence	denotes	Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings.
T8654	9303-9307	JJ	denotes	Wild
T8656	9307-9308	HYPH	denotes	-
T8655	9308-9312	NN	denotes	type
T8658	9313-9316	CC	denotes	and
T8659	9317-9327	JJ	denotes	homozygous
T8660	9328-9338	NN	denotes	CbCln3Δex7
T8662	9338-9339	HYPH	denotes	/
T8663	9339-9340	CD	denotes	8
T8664	9341-9349	JJ	denotes	confocal
T8661	9350-9356	NNS	denotes	images
T8665	9357-9361	VBD	denotes	were
T8657	9362-9370	VBN	denotes	captured
T8666	9371-9375	IN	denotes	with
T8667	9376-9385	JJ	denotes	identical
T8669	9386-9394	NN	denotes	exposure
T8668	9395-9403	NNS	denotes	settings
T8670	9403-9404	.	denotes	.
T8671	9404-9424	sentence	denotes	60 × magnification.
T8672	9405-9407	CD	denotes	60
T8674	9408-9409	SYM	denotes	×
T8673	9410-9423	NN	denotes	magnification
T8675	9423-9424	.	denotes	.
T2096	9425-9431	IN	denotes	During
T2098	9432-9445	JJ	denotes	sub-confluent
T2100	9446-9452	NN	denotes	growth
T2099	9453-9463	NNS	denotes	conditions
T2101	9463-9465	,	denotes	,
T2102	9465-9472	CC	denotes	neither
T2104	9473-9477	JJ	denotes	wild
T2105	9477-9478	HYPH	denotes	-
T2103	9478-9482	NN	denotes	type
T2107	9483-9486	CC	denotes	nor
T2108	9487-9497	JJ	denotes	homozygous
T2109	9498-9508	NN	denotes	CbCln3Δex7
T2110	9508-9509	HYPH	denotes	/
T2111	9509-9510	CD	denotes	8
T2106	9511-9516	NNS	denotes	cells
T2097	9517-9526	VBD	denotes	displayed
T2112	9527-9543	NN	denotes	autofluorescence
T2114	9544-9546	CC	denotes	or
T2115	9547-9554	NN	denotes	subunit
T2116	9555-9556	NN	denotes	c
T2117	9557-9566	NN	denotes	inclusion
T2113	9567-9576	NN	denotes	formation
T2118	9577-9578	-LRB-	denotes	(
T2120	9578-9582	NNS	denotes	data
T2121	9583-9586	RB	denotes	not
T2119	9587-9592	VBN	denotes	shown
T2122	9592-9593	-RRB-	denotes	)
T2123	9593-9594	.	denotes	.
T2124	9594-9823	sentence	denotes	However, when cells were aged at confluency (3+ days post-confluency), homozygous CbCln3Δex7/8 cellular subunit c levels were elevated beyond normal wild-type levels by immunostaining (Fig. 4a) and immunoblot analysis (Fig. 4b).
T2125	9595-9602	RB	denotes	However
T2127	9602-9604	,	denotes	,
T2128	9604-9608	WRB	denotes	when
T2130	9609-9614	NNS	denotes	cells
T2131	9615-9619	VBD	denotes	were
T2129	9620-9624	VBN	denotes	aged
T2132	9625-9627	IN	denotes	at
T2133	9628-9638	NN	denotes	confluency
T2134	9639-9640	-LRB-	denotes	(
T2135	9640-9641	CD	denotes	3
T2137	9641-9642	SYM	denotes	+
T2136	9643-9647	NNS	denotes	days
T2138	9648-9663	RB	denotes	post-confluency
T2139	9663-9664	-RRB-	denotes	)
T2140	9664-9666	,	denotes	,
T2141	9666-9676	JJ	denotes	homozygous
T2143	9677-9687	NN	denotes	CbCln3Δex7
T2144	9687-9688	HYPH	denotes	/
T2145	9688-9689	CD	denotes	8
T2146	9690-9698	JJ	denotes	cellular
T2147	9699-9706	NN	denotes	subunit
T2148	9707-9708	NN	denotes	c
T2142	9709-9715	NNS	denotes	levels
T2149	9716-9720	VBD	denotes	were
T2126	9721-9729	VBN	denotes	elevated
T2150	9730-9736	IN	denotes	beyond
T2151	9737-9743	JJ	denotes	normal
T2153	9744-9748	JJ	denotes	wild
T2155	9748-9749	HYPH	denotes	-
T2154	9749-9753	NN	denotes	type
T2152	9754-9760	NNS	denotes	levels
T2156	9761-9763	IN	denotes	by
T2157	9764-9778	NN	denotes	immunostaining
T2158	9779-9780	-LRB-	denotes	(
T2160	9780-9784	NN	denotes	Fig.
T2159	9785-9787	NN	denotes	4a
T2161	9787-9788	-RRB-	denotes	)
T2162	9789-9792	CC	denotes	and
T2163	9793-9803	NN	denotes	immunoblot
T2164	9804-9812	NN	denotes	analysis
T2165	9813-9814	-LRB-	denotes	(
T2167	9814-9818	NN	denotes	Fig.
T2166	9819-9821	NN	denotes	4b
T2168	9821-9822	-RRB-	denotes	)
T2169	9822-9823	.	denotes	.
T2170	9823-9938	sentence	denotes	Autofluorescent signal sometimes overlapped with subunit c signal, but also was elevated more diffusely (Fig. 4a).
T2171	9824-9839	JJ	denotes	Autofluorescent
T2172	9840-9846	NN	denotes	signal
T2174	9847-9856	RB	denotes	sometimes
T2173	9857-9867	VBD	denotes	overlapped
T2175	9868-9872	IN	denotes	with
T2176	9873-9880	NN	denotes	subunit
T2177	9881-9882	NN	denotes	c
T2178	9883-9889	NN	denotes	signal
T2179	9889-9891	,	denotes	,
T2180	9891-9894	CC	denotes	but
T2181	9895-9899	RB	denotes	also
T2182	9900-9903	VBD	denotes	was
T2183	9904-9912	JJ	denotes	elevated
T2184	9913-9917	RBR	denotes	more
T2185	9918-9927	RB	denotes	diffusely
T2186	9928-9929	-LRB-	denotes	(
T2188	9929-9933	NN	denotes	Fig.
T2187	9934-9936	NN	denotes	4a
T2189	9936-9937	-RRB-	denotes	)
T2190	9937-9938	.	denotes	.
T2191	9938-10300	sentence	denotes	Moreover, although multilamellar "fingerprint" profiles were not detected, confluency-aged homozygous CbCln3Δex7/8 cells displayed numerous ultrastructural abnormalities including electron dense inclusions characteristic of lipofuscin and large autophagosomes that contained dense core structures, degenerating mitochondria, and many smaller vesicles (Fig. 4c).
T2192	9939-9947	RB	denotes	Moreover
T2194	9947-9949	,	denotes	,
T2195	9949-9957	IN	denotes	although
T2197	9958-9971	JJ	denotes	multilamellar
T2199	9972-9973	``	denotes	"
T2200	9973-9984	NN	denotes	fingerprint
T2201	9984-9985	''	denotes	"
T2198	9986-9994	NNS	denotes	profiles
T2202	9995-9999	VBD	denotes	were
T2203	10000-10003	RB	denotes	not
T2196	10004-10012	VBN	denotes	detected
T2204	10012-10014	,	denotes	,
T2205	10014-10024	JJ	denotes	confluency
T2207	10024-10025	HYPH	denotes	-
T2206	10025-10029	VBN	denotes	aged
T2209	10030-10040	JJ	denotes	homozygous
T2210	10041-10051	NN	denotes	CbCln3Δex7
T2211	10051-10052	HYPH	denotes	/
T2212	10052-10053	CD	denotes	8
T2208	10054-10059	NNS	denotes	cells
T2193	10060-10069	VBD	denotes	displayed
T2213	10070-10078	JJ	denotes	numerous
T2215	10079-10094	JJ	denotes	ultrastructural
T2214	10095-10108	NNS	denotes	abnormalities
T2216	10109-10118	VBG	denotes	including
T2217	10119-10127	NN	denotes	electron
T2218	10128-10133	JJ	denotes	dense
T2219	10134-10144	NNS	denotes	inclusions
T2220	10145-10159	JJ	denotes	characteristic
T2221	10160-10162	IN	denotes	of
T2222	10163-10173	NN	denotes	lipofuscin
T2223	10174-10177	CC	denotes	and
T2224	10178-10183	JJ	denotes	large
T2225	10184-10198	NNS	denotes	autophagosomes
T2226	10199-10203	WDT	denotes	that
T2227	10204-10213	VBD	denotes	contained
T2228	10214-10219	JJ	denotes	dense
T2230	10220-10224	NN	denotes	core
T2229	10225-10235	NNS	denotes	structures
T2231	10235-10237	,	denotes	,
T2232	10237-10249	VBG	denotes	degenerating
T2233	10250-10262	NNS	denotes	mitochondria
T2234	10262-10264	,	denotes	,
T2235	10264-10267	CC	denotes	and
T2236	10268-10272	JJ	denotes	many
T2238	10273-10280	JJR	denotes	smaller
T2237	10281-10289	NNS	denotes	vesicles
T2239	10290-10291	-LRB-	denotes	(
T2241	10291-10295	NNP	denotes	Fig.
T2240	10296-10298	NN	denotes	4c
T2242	10298-10299	-RRB-	denotes	)
T2243	10299-10300	.	denotes	.
T2244	10300-10419	sentence	denotes	Inclusion bodies and autophagosomes were infrequently observed in confluency-aged wild-type cultures (data not shown).
T2245	10301-10310	NN	denotes	Inclusion
T2246	10311-10317	NNS	denotes	bodies
T2248	10318-10321	CC	denotes	and
T2249	10322-10336	NNS	denotes	autophagosomes
T2250	10337-10341	VBD	denotes	were
T2251	10342-10354	RB	denotes	infrequently
T2247	10355-10363	VBN	denotes	observed
T2252	10364-10366	IN	denotes	in
T2253	10367-10377	NN	denotes	confluency
T2255	10377-10378	HYPH	denotes	-
T2254	10378-10382	VBN	denotes	aged
T2257	10383-10387	JJ	denotes	wild
T2259	10387-10388	HYPH	denotes	-
T2258	10388-10392	NN	denotes	type
T2256	10393-10401	NNS	denotes	cultures
T2260	10402-10403	-LRB-	denotes	(
T2262	10403-10407	NNS	denotes	data
T2263	10408-10411	RB	denotes	not
T2261	10412-10417	VBN	denotes	shown
T2264	10417-10418	-RRB-	denotes	)
T2265	10418-10419	.	denotes	.
T8805	10430-10437	NN	denotes	Subunit
T8806	10438-10439	NN	denotes	c
T8807	10440-10452	NN	denotes	accumulation
T8808	10453-10455	IN	denotes	in
T8809	10456-10466	JJ	denotes	homozygous
T8811	10467-10477	NN	denotes	CbCln3Δex7
T8812	10477-10478	HYPH	denotes	/
T8813	10478-10479	CD	denotes	8
T8814	10480-10490	JJ	denotes	cerebellar
T8815	10491-10500	NN	denotes	precursor
T8810	10501-10506	NNS	denotes	cells
T8816	10506-10634	sentence	denotes	a. Subunit c immunostaining and autofluorescence of 7-day confluency-aged wild-type and homozygous CbCln3Δex7/8 cells is shown.
T8817	10507-10508	LS	denotes	a
T8819	10508-10509	.	denotes	.
T8820	10510-10517	NN	denotes	Subunit
T8821	10518-10519	NN	denotes	c
T8822	10520-10534	NN	denotes	immunostaining
T8823	10535-10538	CC	denotes	and
T8824	10539-10555	NN	denotes	autofluorescence
T8825	10556-10558	IN	denotes	of
T8826	10559-10560	CD	denotes	7
T8828	10560-10561	HYPH	denotes	-
T8827	10561-10564	NN	denotes	day
T8829	10565-10575	NN	denotes	confluency
T8831	10575-10576	HYPH	denotes	-
T8830	10576-10580	VBN	denotes	aged
T8833	10581-10585	JJ	denotes	wild
T8835	10585-10586	HYPH	denotes	-
T8834	10586-10590	NN	denotes	type
T8836	10591-10594	CC	denotes	and
T8837	10595-10605	JJ	denotes	homozygous
T8838	10606-10616	NN	denotes	CbCln3Δex7
T8839	10616-10617	HYPH	denotes	/
T8840	10617-10618	CD	denotes	8
T8832	10619-10624	NNS	denotes	cells
T8841	10625-10627	VBZ	denotes	is
T8818	10628-10633	VBN	denotes	shown
T8842	10633-10634	.	denotes	.
T8843	10634-10730	sentence	denotes	Wild-type cultures (CbCln3+/+) exhibited limited subunit c immunostaining and autofluorescence.
T8844	10635-10639	JJ	denotes	Wild
T8846	10639-10640	HYPH	denotes	-
T8845	10640-10644	NN	denotes	type
T8847	10645-10653	NNS	denotes	cultures
T8849	10654-10655	-LRB-	denotes	(
T8850	10655-10661	NN	denotes	CbCln3
T8851	10661-10662	SYM	denotes	+
T8852	10662-10663	HYPH	denotes	/
T8853	10663-10664	SYM	denotes	+
T8854	10664-10665	-RRB-	denotes	)
T8848	10666-10675	VBD	denotes	exhibited
T8855	10676-10683	JJ	denotes	limited
T8857	10684-10691	NN	denotes	subunit
T8856	10692-10693	NN	denotes	c
T8858	10694-10708	NN	denotes	immunostaining
T8859	10709-10712	CC	denotes	and
T8860	10713-10729	NN	denotes	autofluorescence
T8861	10729-10730	.	denotes	.
T8862	10730-10802	sentence	denotes	However, CbCln3Δex7/8/Δex7/8 cells contained numerous subunit c puncta.
T8863	10731-10738	RB	denotes	However
T8865	10738-10740	,	denotes	,
T8866	10740-10750	NN	denotes	CbCln3Δex7
T8868	10750-10751	HYPH	denotes	/
T8869	10751-10752	CD	denotes	8
T8870	10752-10753	HYPH	denotes	/
T8867	10753-10757	NN	denotes	Δex7
T8872	10757-10758	HYPH	denotes	/
T8873	10758-10759	CD	denotes	8
T8871	10760-10765	NNS	denotes	cells
T8864	10766-10775	VBD	denotes	contained
T8874	10776-10784	JJ	denotes	numerous
T8876	10785-10792	NN	denotes	subunit
T8877	10793-10794	NN	denotes	c
T8875	10795-10801	NNS	denotes	puncta
T8878	10801-10802	.	denotes	.
T8879	10802-10949	sentence	denotes	Autofluorescence (7 days AF) was also significantly elevated (right panels), although limited overlap with subunit c puncta was observed (arrows).
T8880	10803-10819	NN	denotes	Autofluorescence
T8882	10820-10821	-LRB-	denotes	(
T8884	10821-10822	CD	denotes	7
T8885	10823-10827	NNS	denotes	days
T8883	10828-10830	NN	denotes	AF
T8886	10830-10831	-RRB-	denotes	)
T8881	10832-10835	VBD	denotes	was
T8887	10836-10840	RB	denotes	also
T8888	10841-10854	RB	denotes	significantly
T8889	10855-10863	VBN	denotes	elevated
T8890	10864-10865	-LRB-	denotes	(
T8892	10865-10870	JJ	denotes	right
T8891	10871-10877	NNS	denotes	panels
T8893	10877-10878	-RRB-	denotes	)
T8894	10878-10880	,	denotes	,
T8895	10880-10888	IN	denotes	although
T8897	10889-10896	JJ	denotes	limited
T8898	10897-10904	NN	denotes	overlap
T8899	10905-10909	IN	denotes	with
T8900	10910-10917	NN	denotes	subunit
T8901	10918-10919	NN	denotes	c
T8902	10920-10926	NNS	denotes	puncta
T8903	10927-10930	VBD	denotes	was
T8896	10931-10939	VBN	denotes	observed
T8904	10940-10941	-LRB-	denotes	(
T8905	10941-10947	NNS	denotes	arrows
T8906	10947-10948	-RRB-	denotes	)
T8907	10948-10949	.	denotes	.
T8908	10949-10969	sentence	denotes	40 × magnification.
T8909	10950-10952	CD	denotes	40
T8911	10953-10954	SYM	denotes	×
T8910	10955-10968	NN	denotes	magnification
T8912	10968-10969	.	denotes	.
T8913	10969-11072	sentence	denotes	b. Immunoblot analysis of subunit c protein at sub-confluency or 7-day confluency incubation is shown.
T8914	10970-10971	LS	denotes	b
T8916	10971-10972	.	denotes	.
T8917	10973-10983	NN	denotes	Immunoblot
T8918	10984-10992	NN	denotes	analysis
T8919	10993-10995	IN	denotes	of
T8920	10996-11003	NN	denotes	subunit
T8921	11004-11005	NN	denotes	c
T8922	11006-11013	NN	denotes	protein
T8923	11014-11016	IN	denotes	at
T8924	11017-11031	NN	denotes	sub-confluency
T8926	11032-11034	CC	denotes	or
T8927	11035-11036	CD	denotes	7
T8929	11036-11037	HYPH	denotes	-
T8928	11037-11040	NN	denotes	day
T8930	11041-11051	NN	denotes	confluency
T8925	11052-11062	NN	denotes	incubation
T8931	11063-11065	VBZ	denotes	is
T8915	11066-11071	VBN	denotes	shown
T8932	11071-11072	.	denotes	.
T8933	11072-11247	sentence	denotes	Total protein extracts from sub-confluency wild-type (+/+) and homozygous mutant (Δex7/8/Δex7/8) cultures contained approximately equal levels of subunit c protein (α-sub c).
T8934	11073-11078	JJ	denotes	Total
T8936	11079-11086	NN	denotes	protein
T8935	11087-11095	NNS	denotes	extracts
T8938	11096-11100	IN	denotes	from
T8939	11101-11115	NN	denotes	sub-confluency
T8941	11116-11120	JJ	denotes	wild
T8943	11120-11121	HYPH	denotes	-
T8942	11121-11125	NN	denotes	type
T8944	11126-11127	-LRB-	denotes	(
T8945	11127-11128	SYM	denotes	+
T8947	11128-11129	HYPH	denotes	/
T8946	11129-11130	SYM	denotes	+
T8948	11130-11131	-RRB-	denotes	)
T8949	11132-11135	CC	denotes	and
T8950	11136-11146	JJ	denotes	homozygous
T8951	11147-11153	NN	denotes	mutant
T8952	11154-11155	-LRB-	denotes	(
T8953	11155-11159	NN	denotes	Δex7
T8954	11159-11160	HYPH	denotes	/
T8955	11160-11161	CD	denotes	8
T8956	11161-11162	HYPH	denotes	/
T8957	11162-11166	NN	denotes	Δex7
T8958	11166-11167	HYPH	denotes	/
T8959	11167-11168	CD	denotes	8
T8960	11168-11169	-RRB-	denotes	)
T8940	11170-11178	NNS	denotes	cultures
T8937	11179-11188	VBD	denotes	contained
T8961	11189-11202	RB	denotes	approximately
T8962	11203-11208	JJ	denotes	equal
T8963	11209-11215	NNS	denotes	levels
T8964	11216-11218	IN	denotes	of
T8965	11219-11226	NN	denotes	subunit
T8966	11227-11228	NN	denotes	c
T8967	11229-11236	NN	denotes	protein
T8968	11237-11238	-LRB-	denotes	(
T8969	11238-11239	NN	denotes	α
T8971	11239-11240	HYPH	denotes	-
T8972	11240-11243	JJ	denotes	sub
T8970	11244-11245	NN	denotes	c
T8973	11245-11246	-RRB-	denotes	)
T8974	11246-11247	.	denotes	.
T8975	11247-11410	sentence	denotes	7-day confluency extract from homozygous CbCln3Δex7/8 cells (Δex7/8/Δex7/8) had elevated levels of subunit c protein (~1.5X), relative to wild-type extract (+/+).
T8976	11248-11249	CD	denotes	7
T8978	11249-11250	HYPH	denotes	-
T8977	11250-11253	NN	denotes	day
T8979	11254-11264	NN	denotes	confluency
T8980	11265-11272	NN	denotes	extract
T8982	11273-11277	IN	denotes	from
T8983	11278-11288	JJ	denotes	homozygous
T8985	11289-11299	NN	denotes	CbCln3Δex7
T8986	11299-11300	HYPH	denotes	/
T8987	11300-11301	CD	denotes	8
T8984	11302-11307	NNS	denotes	cells
T8988	11308-11309	-LRB-	denotes	(
T8989	11309-11313	NN	denotes	Δex7
T8990	11313-11314	HYPH	denotes	/
T8991	11314-11315	CD	denotes	8
T8992	11315-11316	HYPH	denotes	/
T8993	11316-11320	NN	denotes	Δex7
T8994	11320-11321	HYPH	denotes	/
T8995	11321-11322	CD	denotes	8
T8996	11322-11323	-RRB-	denotes	)
T8981	11324-11327	VBD	denotes	had
T8997	11328-11336	VBN	denotes	elevated
T8998	11337-11343	NNS	denotes	levels
T8999	11344-11346	IN	denotes	of
T9000	11347-11354	NN	denotes	subunit
T9001	11355-11356	NN	denotes	c
T9002	11357-11364	NN	denotes	protein
T9003	11365-11366	-LRB-	denotes	(
T9004	11366-11367	SYM	denotes	~
T9005	11367-11370	CD	denotes	1.5
T9006	11370-11371	SYM	denotes	X
T9007	11371-11372	-RRB-	denotes	)
T9008	11372-11374	,	denotes	,
T9009	11374-11382	JJ	denotes	relative
T9010	11383-11385	IN	denotes	to
T9011	11386-11390	JJ	denotes	wild
T9013	11390-11391	HYPH	denotes	-
T9012	11391-11395	NN	denotes	type
T9014	11396-11403	NN	denotes	extract
T9015	11404-11405	-LRB-	denotes	(
T9016	11405-11406	SYM	denotes	+
T9018	11406-11407	HYPH	denotes	/
T9017	11407-11408	SYM	denotes	+
T9019	11408-11409	-RRB-	denotes	)
T9020	11409-11410	.	denotes	.
T9021	11410-11486	sentence	denotes	Protein levels were normalized to cytochrome c oxidase subunit IV (α-cox4).
T9022	11411-11418	NN	denotes	Protein
T9023	11419-11425	NNS	denotes	levels
T9025	11426-11430	VBD	denotes	were
T9024	11431-11441	VBN	denotes	normalized
T9026	11442-11444	IN	denotes	to
T9027	11445-11455	NN	denotes	cytochrome
T9028	11456-11457	NN	denotes	c
T9029	11458-11465	NN	denotes	oxidase
T9030	11466-11473	NN	denotes	subunit
T9031	11474-11476	CD	denotes	IV
T9032	11477-11478	-LRB-	denotes	(
T9033	11478-11479	NN	denotes	α
T9035	11479-11480	HYPH	denotes	-
T9034	11480-11484	NN	denotes	cox4
T9036	11484-11485	-RRB-	denotes	)
T9037	11485-11486	.	denotes	.
T9038	11486-11581	sentence	denotes	c. TEM analysis of inclusions in 7-day confluency-aged homozygous CbCln3Δex7/8 cells is shown.
T9039	11487-11488	LS	denotes	c
T9041	11488-11489	.	denotes	.
T9042	11490-11493	NN	denotes	TEM
T9043	11494-11502	NN	denotes	analysis
T9044	11503-11505	IN	denotes	of
T9045	11506-11516	NNS	denotes	inclusions
T9046	11517-11519	IN	denotes	in
T9047	11520-11521	CD	denotes	7
T9049	11521-11522	HYPH	denotes	-
T9048	11522-11525	NN	denotes	day
T9050	11526-11536	NN	denotes	confluency
T9052	11536-11537	HYPH	denotes	-
T9051	11537-11541	VBN	denotes	aged
T9054	11542-11552	JJ	denotes	homozygous
T9055	11553-11563	NN	denotes	CbCln3Δex7
T9056	11563-11564	HYPH	denotes	/
T9057	11564-11565	CD	denotes	8
T9053	11566-11571	NNS	denotes	cells
T9058	11572-11574	VBZ	denotes	is
T9040	11575-11580	VBN	denotes	shown
T9059	11580-11581	.	denotes	.
T9060	11581-11771	sentence	denotes	A large autophagosome contained by double membrane (arrows) is filled with degenerating mitochondria (Md), electron dense cores (left and right of *) and other smaller vesicular structures.
T9061	11582-11583	DT	denotes	A
T9063	11584-11589	JJ	denotes	large
T9062	11590-11603	NN	denotes	autophagosome
T9065	11604-11613	VBN	denotes	contained
T9066	11614-11616	IN	denotes	by
T9067	11617-11623	JJ	denotes	double
T9068	11624-11632	NN	denotes	membrane
T9069	11633-11634	-LRB-	denotes	(
T9070	11634-11640	NNS	denotes	arrows
T9071	11640-11641	-RRB-	denotes	)
T9072	11642-11644	VBZ	denotes	is
T9064	11645-11651	VBN	denotes	filled
T9073	11652-11656	IN	denotes	with
T9074	11657-11669	VBG	denotes	degenerating
T9075	11670-11682	NNS	denotes	mitochondria
T9076	11683-11684	-LRB-	denotes	(
T9077	11684-11686	NN	denotes	Md
T9078	11686-11687	-RRB-	denotes	)
T9079	11687-11689	,	denotes	,
T9080	11689-11697	NN	denotes	electron
T9082	11698-11703	JJ	denotes	dense
T9081	11704-11709	NNS	denotes	cores
T9083	11710-11711	-LRB-	denotes	(
T9084	11711-11715	JJ	denotes	left
T9085	11716-11719	CC	denotes	and
T9086	11720-11725	JJ	denotes	right
T9087	11726-11728	IN	denotes	of
T9088	11729-11730	SYM	denotes	*
T9089	11730-11731	-RRB-	denotes	)
T9090	11732-11735	CC	denotes	and
T9091	11736-11741	JJ	denotes	other
T9093	11742-11749	JJR	denotes	smaller
T9094	11750-11759	JJ	denotes	vesicular
T9092	11760-11770	NNS	denotes	structures
T9095	11770-11771	.	denotes	.
T9096	11771-11857	sentence	denotes	A large electron-dense inclusion, with a lipofuscin (Ln) appearance, is also present.
T9097	11772-11773	DT	denotes	A
T9099	11774-11779	JJ	denotes	large
T9100	11780-11788	NN	denotes	electron
T9102	11788-11789	HYPH	denotes	-
T9101	11789-11794	JJ	denotes	dense
T9098	11795-11804	NN	denotes	inclusion
T9104	11804-11806	,	denotes	,
T9105	11806-11810	IN	denotes	with
T9106	11811-11812	DT	denotes	a
T9108	11813-11823	NN	denotes	lipofuscin
T9109	11824-11825	-LRB-	denotes	(
T9110	11825-11827	NN	denotes	Ln
T9111	11827-11828	-RRB-	denotes	)
T9107	11829-11839	NN	denotes	appearance
T9112	11839-11841	,	denotes	,
T9103	11841-11843	VBZ	denotes	is
T9113	11844-11848	RB	denotes	also
T9114	11849-11856	JJ	denotes	present
T9115	11856-11857	.	denotes	.
T9116	11857-11874	sentence	denotes	M, mitochondria.
T9117	11858-11859	NN	denotes	M
T9118	11859-11861	,	denotes	,
T9119	11861-11873	NNS	denotes	mitochondria
T9120	11873-11874	.	denotes	.
T9121	11874-11898	sentence	denotes	10,000 × magnification.
T9122	11875-11881	CD	denotes	10,000
T9124	11882-11883	SYM	denotes	×
T9123	11884-11897	NN	denotes	magnification
T9125	11897-11898	.	denotes	.
T2526	11900-11910	JJ	denotes	Homozygous
T2528	11911-11921	NN	denotes	CbCln3Δex7
T2529	11921-11922	HYPH	denotes	/
T2530	11922-11923	CD	denotes	8
T2527	11924-11929	NNS	denotes	cells
T2532	11930-11933	CC	denotes	and
T2533	11934-11942	NN	denotes	Cln3Δex7
T2535	11942-11943	HYPH	denotes	/
T2536	11943-11944	CD	denotes	8
T2537	11945-11950	VB	denotes	knock
T2538	11950-11951	HYPH	denotes	-
T2539	11951-11953	RP	denotes	in
T2534	11954-11958	NNS	denotes	mice
T2531	11959-11966	VBP	denotes	process
T2540	11967-11976	NN	denotes	cathepsin
T2541	11977-11978	NN	denotes	D
T2542	11979-11989	RB	denotes	abnormally
T2543	11989-12182	sentence	denotes	We next investigated the basis for subunit c accumulation, testing the hypothesis that cathepsin D is abnormal since it is required for ATP synthase subunit c degradation in the lysosome [13].
T2544	11990-11992	PRP	denotes	We
T2546	11993-11997	RB	denotes	next
T2545	11998-12010	VBD	denotes	investigated
T2547	12011-12014	DT	denotes	the
T2548	12015-12020	NN	denotes	basis
T2549	12021-12024	IN	denotes	for
T2550	12025-12032	NN	denotes	subunit
T2551	12033-12034	NN	denotes	c
T2552	12035-12047	NN	denotes	accumulation
T2553	12047-12049	,	denotes	,
T2554	12049-12056	VBG	denotes	testing
T2555	12057-12060	DT	denotes	the
T2556	12061-12071	NN	denotes	hypothesis
T2557	12072-12076	IN	denotes	that
T2559	12077-12086	NN	denotes	cathepsin
T2560	12087-12088	NN	denotes	D
T2558	12089-12091	VBZ	denotes	is
T2561	12092-12100	JJ	denotes	abnormal
T2562	12101-12106	IN	denotes	since
T2564	12107-12109	PRP	denotes	it
T2563	12110-12112	VBZ	denotes	is
T2565	12113-12121	JJ	denotes	required
T2566	12122-12125	IN	denotes	for
T2567	12126-12129	NN	denotes	ATP
T2569	12130-12138	NN	denotes	synthase
T2570	12139-12146	NN	denotes	subunit
T2571	12147-12148	NN	denotes	c
T2568	12149-12160	NN	denotes	degradation
T2572	12161-12163	IN	denotes	in
T2573	12164-12167	DT	denotes	the
T2574	12168-12176	NN	denotes	lysosome
T2575	12177-12178	-LRB-	denotes	[
T2576	12178-12180	CD	denotes	13
T2577	12180-12181	-RRB-	denotes	]
T2578	12181-12182	.	denotes	.
T2579	12182-12384	sentence	denotes	We first tested cathepsin D transport and processing in homozygous CbCln3Δex7/8 cells and Cln3Δex7/8 mice using anti-cathepsin D antibody that recognizes unprocessed and processed cathepsin D isoforms.
T2580	12183-12185	PRP	denotes	We
T2582	12186-12191	RB	denotes	first
T2581	12192-12198	VBD	denotes	tested
T2583	12199-12208	NN	denotes	cathepsin
T2584	12209-12210	NN	denotes	D
T2585	12211-12220	NN	denotes	transport
T2586	12221-12224	CC	denotes	and
T2587	12225-12235	NN	denotes	processing
T2588	12236-12238	IN	denotes	in
T2589	12239-12249	JJ	denotes	homozygous
T2591	12250-12260	NN	denotes	CbCln3Δex7
T2592	12260-12261	HYPH	denotes	/
T2593	12261-12262	CD	denotes	8
T2590	12263-12268	NNS	denotes	cells
T2594	12269-12272	CC	denotes	and
T2595	12273-12281	NN	denotes	Cln3Δex7
T2597	12281-12282	HYPH	denotes	/
T2598	12282-12283	CD	denotes	8
T2596	12284-12288	NNS	denotes	mice
T2599	12289-12294	VBG	denotes	using
T2600	12295-12309	JJ	denotes	anti-cathepsin
T2601	12310-12311	NN	denotes	D
T2602	12312-12320	NN	denotes	antibody
T2603	12321-12325	WDT	denotes	that
T2604	12326-12336	VBZ	denotes	recognizes
T2605	12337-12348	JJ	denotes	unprocessed
T2607	12349-12352	CC	denotes	and
T2608	12353-12362	VBN	denotes	processed
T2609	12363-12372	NN	denotes	cathepsin
T2610	12373-12374	NN	denotes	D
T2606	12375-12383	NNS	denotes	isoforms
T2611	12383-12384	.	denotes	.
T2612	12384-12633	sentence	denotes	Immunostaining of wild-type and homozygous CbCln3Δex7/8 cells revealed a perinuclear and punctate vesicular cathepsin D distribution, consistent with its transport and processing through the secretory pathway and delivery to the lysosome (Fig. 5a).
T2613	12385-12399	NN	denotes	Immunostaining
T2615	12400-12402	IN	denotes	of
T2616	12403-12407	JJ	denotes	wild
T2618	12407-12408	HYPH	denotes	-
T2617	12408-12412	NN	denotes	type
T2620	12413-12416	CC	denotes	and
T2621	12417-12427	JJ	denotes	homozygous
T2622	12428-12438	NN	denotes	CbCln3Δex7
T2623	12438-12439	HYPH	denotes	/
T2624	12439-12440	CD	denotes	8
T2619	12441-12446	NNS	denotes	cells
T2614	12447-12455	VBD	denotes	revealed
T2625	12456-12457	DT	denotes	a
T2627	12458-12469	JJ	denotes	perinuclear
T2628	12470-12473	CC	denotes	and
T2629	12474-12482	JJ	denotes	punctate
T2630	12483-12492	JJ	denotes	vesicular
T2631	12493-12502	NN	denotes	cathepsin
T2632	12503-12504	NN	denotes	D
T2626	12505-12517	NN	denotes	distribution
T2633	12517-12519	,	denotes	,
T2634	12519-12529	JJ	denotes	consistent
T2635	12530-12534	IN	denotes	with
T2636	12535-12538	PRP$	denotes	its
T2637	12539-12548	NN	denotes	transport
T2638	12549-12552	CC	denotes	and
T2639	12553-12563	NN	denotes	processing
T2640	12564-12571	IN	denotes	through
T2641	12572-12575	DT	denotes	the
T2643	12576-12585	JJ	denotes	secretory
T2642	12586-12593	NN	denotes	pathway
T2644	12594-12597	CC	denotes	and
T2645	12598-12606	NN	denotes	delivery
T2646	12607-12609	IN	denotes	to
T2647	12610-12613	DT	denotes	the
T2648	12614-12622	NN	denotes	lysosome
T2649	12623-12624	-LRB-	denotes	(
T2651	12624-12628	NN	denotes	Fig.
T2650	12629-12631	NN	denotes	5a
T2652	12631-12632	-RRB-	denotes	)
T2653	12632-12633	.	denotes	.
T2654	12633-12776	sentence	denotes	However, in homozygous CbCln3Δex7/8 cells, cathepsin D distribution was less vesicular and more perinuclear-clustered than in wild-type cells.
T2655	12634-12641	RB	denotes	However
T2657	12641-12643	,	denotes	,
T2658	12643-12645	IN	denotes	in
T2659	12646-12656	JJ	denotes	homozygous
T2661	12657-12667	NN	denotes	CbCln3Δex7
T2662	12667-12668	HYPH	denotes	/
T2663	12668-12669	CD	denotes	8
T2660	12670-12675	NNS	denotes	cells
T2664	12675-12677	,	denotes	,
T2665	12677-12686	NN	denotes	cathepsin
T2666	12687-12688	NN	denotes	D
T2667	12689-12701	NN	denotes	distribution
T2656	12702-12705	VBD	denotes	was
T2668	12706-12710	RBR	denotes	less
T2669	12711-12720	JJ	denotes	vesicular
T2670	12721-12724	CC	denotes	and
T2671	12725-12729	RBR	denotes	more
T2673	12730-12741	JJ	denotes	perinuclear
T2674	12741-12742	HYPH	denotes	-
T2672	12742-12751	VBN	denotes	clustered
T2675	12752-12756	IN	denotes	than
T2676	12757-12759	IN	denotes	in
T2677	12760-12764	JJ	denotes	wild
T2679	12764-12765	HYPH	denotes	-
T2678	12765-12769	NN	denotes	type
T2680	12770-12775	NNS	denotes	cells
T2681	12775-12776	.	denotes	.
T2682	12776-12922	sentence	denotes	Immunoblots of homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts also showed altered relative levels of cathepsin D isoforms (Fig. 5b).
T2683	12777-12788	NNS	denotes	Immunoblots
T2685	12789-12791	IN	denotes	of
T2686	12792-12802	JJ	denotes	homozygous
T2688	12803-12813	NN	denotes	CbCln3Δex7
T2690	12813-12814	HYPH	denotes	/
T2691	12814-12815	CD	denotes	8
T2689	12816-12820	NN	denotes	cell
T2692	12821-12824	CC	denotes	and
T2693	12825-12833	NN	denotes	Cln3Δex7
T2695	12833-12834	HYPH	denotes	/
T2696	12834-12835	CD	denotes	8
T2694	12836-12842	NN	denotes	tissue
T2687	12843-12851	NNS	denotes	extracts
T2697	12852-12856	RB	denotes	also
T2684	12857-12863	VBD	denotes	showed
T2698	12864-12871	VBN	denotes	altered
T2700	12872-12880	JJ	denotes	relative
T2699	12881-12887	NNS	denotes	levels
T2701	12888-12890	IN	denotes	of
T2702	12891-12900	NN	denotes	cathepsin
T2703	12901-12902	NN	denotes	D
T2704	12903-12911	NNS	denotes	isoforms
T2705	12912-12913	-LRB-	denotes	(
T2707	12913-12917	NN	denotes	Fig.
T2706	12918-12920	NN	denotes	5b
T2708	12920-12921	-RRB-	denotes	)
T2709	12921-12922	.	denotes	.
T2710	12922-13156	sentence	denotes	Cathepsin D isoforms, identified by relative molecular weights, represent the ~45 kDa precursor, the ~43 kDa intermediate single chain form of the enzyme, and the 31 kDa heavy chain of the double-chain form of the mature enzyme [14].
T2711	12923-12932	NN	denotes	Cathepsin
T2712	12933-12934	NN	denotes	D
T2713	12935-12943	NNS	denotes	isoforms
T2715	12943-12945	,	denotes	,
T2716	12945-12955	VBN	denotes	identified
T2717	12956-12958	IN	denotes	by
T2718	12959-12967	JJ	denotes	relative
T2720	12968-12977	JJ	denotes	molecular
T2719	12978-12985	NNS	denotes	weights
T2721	12985-12987	,	denotes	,
T2714	12987-12996	VBP	denotes	represent
T2722	12997-13000	DT	denotes	the
T2724	13001-13002	SYM	denotes	~
T2725	13002-13004	CD	denotes	45
T2726	13005-13008	NN	denotes	kDa
T2723	13009-13018	NN	denotes	precursor
T2727	13018-13020	,	denotes	,
T2728	13020-13023	DT	denotes	the
T2730	13024-13025	SYM	denotes	~
T2731	13025-13027	CD	denotes	43
T2732	13028-13031	NN	denotes	kDa
T2733	13032-13044	JJ	denotes	intermediate
T2734	13045-13051	JJ	denotes	single
T2735	13052-13057	NN	denotes	chain
T2729	13058-13062	NN	denotes	form
T2736	13063-13065	IN	denotes	of
T2737	13066-13069	DT	denotes	the
T2738	13070-13076	NN	denotes	enzyme
T2739	13076-13078	,	denotes	,
T2740	13078-13081	CC	denotes	and
T2741	13082-13085	DT	denotes	the
T2743	13086-13088	CD	denotes	31
T2744	13089-13092	NN	denotes	kDa
T2745	13093-13098	JJ	denotes	heavy
T2742	13099-13104	NN	denotes	chain
T2746	13105-13107	IN	denotes	of
T2747	13108-13111	DT	denotes	the
T2749	13112-13118	JJ	denotes	double
T2751	13118-13119	HYPH	denotes	-
T2750	13119-13124	NN	denotes	chain
T2748	13125-13129	NN	denotes	form
T2752	13130-13132	IN	denotes	of
T2753	13133-13136	DT	denotes	the
T2755	13137-13143	JJ	denotes	mature
T2754	13144-13150	NN	denotes	enzyme
T2756	13151-13152	-LRB-	denotes	[
T2757	13152-13154	CD	denotes	14
T2758	13154-13155	-RRB-	denotes	]
T2759	13155-13156	.	denotes	.
T2760	13156-13340	sentence	denotes	In homozygous CbCln3Δex7/8 cell and Cln3Δex7/8 tissue extracts, the precursor and heavy chains were reduced, and the single chain was slightly elevated compared to wild-type extracts.
T2761	13157-13159	IN	denotes	In
T2763	13160-13170	JJ	denotes	homozygous
T2765	13171-13181	NN	denotes	CbCln3Δex7
T2767	13181-13182	HYPH	denotes	/
T2768	13182-13183	CD	denotes	8
T2766	13184-13188	NN	denotes	cell
T2769	13189-13192	CC	denotes	and
T2770	13193-13201	NN	denotes	Cln3Δex7
T2772	13201-13202	HYPH	denotes	/
T2773	13202-13203	CD	denotes	8
T2771	13204-13210	NN	denotes	tissue
T2764	13211-13219	NNS	denotes	extracts
T2774	13219-13221	,	denotes	,
T2775	13221-13224	DT	denotes	the
T2776	13225-13234	NN	denotes	precursor
T2777	13235-13238	CC	denotes	and
T2778	13239-13244	JJ	denotes	heavy
T2779	13245-13251	NNS	denotes	chains
T2780	13252-13256	VBD	denotes	were
T2762	13257-13264	VBN	denotes	reduced
T2781	13264-13266	,	denotes	,
T2782	13266-13269	CC	denotes	and
T2783	13270-13273	DT	denotes	the
T2785	13274-13280	JJ	denotes	single
T2784	13281-13286	NN	denotes	chain
T2786	13287-13290	VBD	denotes	was
T2787	13291-13299	RB	denotes	slightly
T2788	13300-13308	JJ	denotes	elevated
T2789	13309-13317	VBN	denotes	compared
T2790	13318-13320	IN	denotes	to
T2791	13321-13325	JJ	denotes	wild
T2793	13325-13326	HYPH	denotes	-
T2792	13326-13330	NN	denotes	type
T2794	13331-13339	NNS	denotes	extracts
T2795	13339-13340	.	denotes	.
T2796	13340-13459	sentence	denotes	The cellular growth media did not contain altered levels of cathepsin D, indicating enzyme secretion was not affected.
T2797	13341-13344	DT	denotes	The
T2799	13345-13353	JJ	denotes	cellular
T2800	13354-13360	NN	denotes	growth
T2798	13361-13366	NNS	denotes	media
T2802	13367-13370	VBD	denotes	did
T2803	13371-13374	RB	denotes	not
T2801	13375-13382	VB	denotes	contain
T2804	13383-13390	VBN	denotes	altered
T2805	13391-13397	NNS	denotes	levels
T2806	13398-13400	IN	denotes	of
T2807	13401-13410	NN	denotes	cathepsin
T2808	13411-13412	NN	denotes	D
T2809	13412-13414	,	denotes	,
T2810	13414-13424	VBG	denotes	indicating
T2811	13425-13431	NN	denotes	enzyme
T2812	13432-13441	NN	denotes	secretion
T2814	13442-13445	VBD	denotes	was
T2815	13446-13449	RB	denotes	not
T2813	13450-13458	VBN	denotes	affected
T2816	13458-13459	.	denotes	.
T2817	13459-13614	sentence	denotes	Heterozygous Cln3Δex7/8 mice and CbCln3Δex7/8 cells were indistinguishable from wild-type, as expected for a recessive disease phenotype (data not shown).
T2818	13460-13472	JJ	denotes	Heterozygous
T2820	13473-13481	NN	denotes	Cln3Δex7
T2821	13481-13482	HYPH	denotes	/
T2822	13482-13483	CD	denotes	8
T2819	13484-13488	NNS	denotes	mice
T2824	13489-13492	CC	denotes	and
T2825	13493-13503	NN	denotes	CbCln3Δex7
T2827	13503-13504	HYPH	denotes	/
T2828	13504-13505	CD	denotes	8
T2826	13506-13511	NNS	denotes	cells
T2823	13512-13516	VBD	denotes	were
T2829	13517-13534	JJ	denotes	indistinguishable
T2830	13535-13539	IN	denotes	from
T2831	13540-13544	JJ	denotes	wild
T2833	13544-13545	HYPH	denotes	-
T2832	13545-13549	NN	denotes	type
T2834	13549-13551	,	denotes	,
T2835	13551-13553	IN	denotes	as
T2836	13554-13562	VBN	denotes	expected
T2837	13563-13566	IN	denotes	for
T2838	13567-13568	DT	denotes	a
T2840	13569-13578	JJ	denotes	recessive
T2841	13579-13586	NN	denotes	disease
T2839	13587-13596	NN	denotes	phenotype
T2842	13597-13598	-LRB-	denotes	(
T2844	13598-13602	NNS	denotes	data
T2845	13603-13606	RB	denotes	not
T2843	13607-13612	VBN	denotes	shown
T2846	13612-13613	-RRB-	denotes	)
T2847	13613-13614	.	denotes	.
T2848	13614-13615	sentence	denotes
T9254	13625-13634	NN	denotes	Cathepsin
T9255	13635-13636	NN	denotes	D
T9256	13637-13649	NN	denotes	localization
T9257	13650-13653	CC	denotes	and
T9258	13654-13664	NN	denotes	processing
T9259	13665-13667	IN	denotes	in
T9260	13668-13672	JJ	denotes	wild
T9262	13672-13673	HYPH	denotes	-
T9261	13673-13677	NN	denotes	type
T9264	13678-13681	CC	denotes	and
T9265	13682-13692	JJ	denotes	homozygous
T9266	13693-13703	NN	denotes	CbCln3Δex7
T9267	13703-13704	HYPH	denotes	/
T9268	13704-13705	CD	denotes	8
T9263	13706-13711	NNS	denotes	cells
T9269	13711-13895	sentence	denotes	a. Immunostaining of wild-type and homozygous CbCln3Δex7/8 precursor cells with anti-cathepsin D antibody, recognizing unprocessed and processed forms of cathepsin D protein is shown.
T9270	13712-13713	LS	denotes	a
T9272	13713-13714	.	denotes	.
T9273	13715-13729	NN	denotes	Immunostaining
T9274	13730-13732	IN	denotes	of
T9275	13733-13737	JJ	denotes	wild
T9277	13737-13738	HYPH	denotes	-
T9276	13738-13742	NN	denotes	type
T9279	13743-13746	CC	denotes	and
T9280	13747-13757	JJ	denotes	homozygous
T9281	13758-13768	NN	denotes	CbCln3Δex7
T9282	13768-13769	HYPH	denotes	/
T9283	13769-13770	CD	denotes	8
T9284	13771-13780	NN	denotes	precursor
T9278	13781-13786	NNS	denotes	cells
T9285	13787-13791	IN	denotes	with
T9286	13792-13806	JJ	denotes	anti-cathepsin
T9287	13807-13808	NN	denotes	D
T9288	13809-13817	NN	denotes	antibody
T9289	13817-13819	,	denotes	,
T9290	13819-13830	VBG	denotes	recognizing
T9291	13831-13842	JJ	denotes	unprocessed
T9293	13843-13846	CC	denotes	and
T9294	13847-13856	VBN	denotes	processed
T9292	13857-13862	NNS	denotes	forms
T9295	13863-13865	IN	denotes	of
T9296	13866-13875	NN	denotes	cathepsin
T9297	13876-13877	NN	denotes	D
T9298	13878-13885	NN	denotes	protein
T9299	13886-13888	VBZ	denotes	is
T9271	13889-13894	VBN	denotes	shown
T9300	13894-13895	.	denotes	.
T9301	13895-13981	sentence	denotes	CbCln3+/+ cells (left panel) exhibited a perinuclear and cytoplasmic punctate signal.
T9302	13896-13902	NN	denotes	CbCln3
T9304	13902-13903	SYM	denotes	+
T9305	13903-13904	HYPH	denotes	/
T9306	13904-13905	SYM	denotes	+
T9303	13906-13911	NNS	denotes	cells
T9308	13912-13913	-LRB-	denotes	(
T9310	13913-13917	JJ	denotes	left
T9309	13918-13923	NN	denotes	panel
T9311	13923-13924	-RRB-	denotes	)
T9307	13925-13934	VBD	denotes	exhibited
T9312	13935-13936	DT	denotes	a
T9314	13937-13948	JJ	denotes	perinuclear
T9315	13949-13952	CC	denotes	and
T9316	13953-13964	JJ	denotes	cytoplasmic
T9317	13965-13973	JJ	denotes	punctate
T9313	13974-13980	NN	denotes	signal
T9318	13980-13981	.	denotes	.
T9319	13981-14153	sentence	denotes	Cathepsin D signal in homozygous CbCln3Δex7/8 cells (right panel) was more often perinuclear, with less cytoplasmic punctate signal, compared to wild-type CbCln3+/+ cells.
T9320	13982-13991	NN	denotes	Cathepsin
T9321	13992-13993	NN	denotes	D
T9322	13994-14000	NN	denotes	signal
T9324	14001-14003	IN	denotes	in
T9325	14004-14014	JJ	denotes	homozygous
T9327	14015-14025	NN	denotes	CbCln3Δex7
T9328	14025-14026	HYPH	denotes	/
T9329	14026-14027	CD	denotes	8
T9326	14028-14033	NNS	denotes	cells
T9330	14034-14035	-LRB-	denotes	(
T9332	14035-14040	JJ	denotes	right
T9331	14041-14046	NN	denotes	panel
T9333	14046-14047	-RRB-	denotes	)
T9323	14048-14051	VBD	denotes	was
T9334	14052-14056	RBR	denotes	more
T9335	14057-14062	RB	denotes	often
T9336	14063-14074	JJ	denotes	perinuclear
T9337	14074-14076	,	denotes	,
T9338	14076-14080	IN	denotes	with
T9339	14081-14085	RBR	denotes	less
T9341	14086-14097	JJ	denotes	cytoplasmic
T9342	14098-14106	NN	denotes	punctate
T9340	14107-14113	NN	denotes	signal
T9343	14113-14115	,	denotes	,
T9344	14115-14123	VBN	denotes	compared
T9345	14124-14126	IN	denotes	to
T9346	14127-14131	JJ	denotes	wild
T9348	14131-14132	HYPH	denotes	-
T9347	14132-14136	NN	denotes	type
T9350	14137-14143	NN	denotes	CbCln3
T9351	14143-14144	SYM	denotes	+
T9352	14144-14145	HYPH	denotes	/
T9353	14145-14146	SYM	denotes	+
T9349	14147-14152	NNS	denotes	cells
T9354	14152-14153	.	denotes	.
T9355	14153-14173	sentence	denotes	40 × magnification.
T9356	14154-14156	CD	denotes	40
T9358	14157-14158	SYM	denotes	×
T9357	14159-14172	NN	denotes	magnification
T9359	14172-14173	.	denotes	.
T9360	14173-14318	sentence	denotes	b. α-Cathepsin D-probed immunoblots of total wild-type versus homozygous Cln3Δex7/8 knock-in tissue or CbCln3Δex7/8 cellular extracts are shown.
T9361	14174-14175	LS	denotes	b
T9363	14175-14176	.	denotes	.
T9364	14177-14178	NN	denotes	α
T9366	14178-14179	HYPH	denotes	-
T9367	14179-14188	NN	denotes	Cathepsin
T9365	14189-14190	NN	denotes	D
T9369	14190-14191	HYPH	denotes	-
T9368	14191-14197	VBN	denotes	probed
T9370	14198-14209	NNS	denotes	immunoblots
T9371	14210-14212	IN	denotes	of
T9372	14213-14218	JJ	denotes	total
T9374	14219-14223	JJ	denotes	wild
T9376	14223-14224	HYPH	denotes	-
T9375	14224-14228	NN	denotes	type
T9377	14229-14235	CC	denotes	versus
T9378	14236-14246	JJ	denotes	homozygous
T9379	14247-14255	NN	denotes	Cln3Δex7
T9381	14255-14256	HYPH	denotes	/
T9382	14256-14257	CD	denotes	8
T9380	14258-14263	VB	denotes	knock
T9383	14263-14264	HYPH	denotes	-
T9384	14264-14266	RP	denotes	in
T9373	14267-14273	NN	denotes	tissue
T9385	14274-14276	CC	denotes	or
T9386	14277-14287	NN	denotes	CbCln3Δex7
T9388	14287-14288	HYPH	denotes	/
T9389	14288-14289	CD	denotes	8
T9390	14290-14298	JJ	denotes	cellular
T9387	14299-14307	NNS	denotes	extracts
T9391	14308-14311	VBP	denotes	are
T9362	14312-14317	VBN	denotes	shown
T9392	14317-14318	.	denotes	.
T9393	14318-14531	sentence	denotes	The ~45 kDa cathepsin D band, representing precursor, was the predominant band in wild-type (wt) tissue and cellular extracts, with lower levels of mature enzyme (single chain, ~43 kDa, and heavy chain, ~31 kDa).
T9394	14319-14322	DT	denotes	The
T9396	14323-14324	SYM	denotes	~
T9397	14324-14326	CD	denotes	45
T9398	14327-14330	NN	denotes	kDa
T9399	14331-14340	NN	denotes	cathepsin
T9400	14341-14342	NN	denotes	D
T9395	14343-14347	NN	denotes	band
T9402	14347-14349	,	denotes	,
T9403	14349-14361	VBG	denotes	representing
T9404	14362-14371	NN	denotes	precursor
T9405	14371-14373	,	denotes	,
T9401	14373-14376	VBD	denotes	was
T9406	14377-14380	DT	denotes	the
T9408	14381-14392	JJ	denotes	predominant
T9407	14393-14397	NN	denotes	band
T9409	14398-14400	IN	denotes	in
T9410	14401-14405	JJ	denotes	wild
T9412	14405-14406	HYPH	denotes	-
T9411	14406-14410	NN	denotes	type
T9413	14411-14412	-LRB-	denotes	(
T9414	14412-14414	NN	denotes	wt
T9415	14414-14415	-RRB-	denotes	)
T9416	14416-14422	NN	denotes	tissue
T9417	14423-14426	CC	denotes	and
T9418	14427-14435	JJ	denotes	cellular
T9419	14436-14444	NNS	denotes	extracts
T9420	14444-14446	,	denotes	,
T9421	14446-14450	IN	denotes	with
T9422	14451-14456	JJR	denotes	lower
T9423	14457-14463	NNS	denotes	levels
T9424	14464-14466	IN	denotes	of
T9425	14467-14473	JJ	denotes	mature
T9426	14474-14480	NN	denotes	enzyme
T9427	14481-14482	-LRB-	denotes	(
T9429	14482-14488	JJ	denotes	single
T9430	14489-14494	NN	denotes	chain
T9431	14494-14496	,	denotes	,
T9432	14496-14497	SYM	denotes	~
T9433	14497-14499	CD	denotes	43
T9428	14500-14503	NN	denotes	kDa
T9434	14503-14505	,	denotes	,
T9435	14505-14508	CC	denotes	and
T9436	14509-14514	JJ	denotes	heavy
T9437	14515-14520	NN	denotes	chain
T9439	14520-14522	,	denotes	,
T9440	14522-14523	SYM	denotes	~
T9441	14523-14525	CD	denotes	31
T9438	14526-14529	NN	denotes	kDa
T9442	14529-14530	-RRB-	denotes	)
T9443	14530-14531	.	denotes	.
T9444	14531-14748	sentence	denotes	Conversely, homozygous Cln3Δex7/8 and CbCln3Δex7/8 mutant (m) extracts exhibited reduced levels of precursor and heavy chain of the double-chain form of the enzyme, with elevated levels of single-chain mature enzyme.
T9445	14532-14542	RB	denotes	Conversely
T9447	14542-14544	,	denotes	,
T9448	14544-14554	JJ	denotes	homozygous
T9450	14555-14563	NN	denotes	Cln3Δex7
T9451	14563-14564	HYPH	denotes	/
T9452	14564-14565	CD	denotes	8
T9453	14566-14569	CC	denotes	and
T9454	14570-14580	NN	denotes	CbCln3Δex7
T9455	14580-14581	HYPH	denotes	/
T9456	14581-14582	CD	denotes	8
T9457	14583-14589	NN	denotes	mutant
T9458	14590-14591	-LRB-	denotes	(
T9459	14591-14592	NN	denotes	m
T9460	14592-14593	-RRB-	denotes	)
T9449	14594-14602	NNS	denotes	extracts
T9446	14603-14612	VBD	denotes	exhibited
T9461	14613-14620	VBN	denotes	reduced
T9462	14621-14627	NNS	denotes	levels
T9463	14628-14630	IN	denotes	of
T9464	14631-14640	NN	denotes	precursor
T9465	14641-14644	CC	denotes	and
T9466	14645-14650	JJ	denotes	heavy
T9467	14651-14656	NN	denotes	chain
T9468	14657-14659	IN	denotes	of
T9469	14660-14663	DT	denotes	the
T9471	14664-14670	JJ	denotes	double
T9473	14670-14671	HYPH	denotes	-
T9472	14671-14676	NN	denotes	chain
T9470	14677-14681	NN	denotes	form
T9474	14682-14684	IN	denotes	of
T9475	14685-14688	DT	denotes	the
T9476	14689-14695	NN	denotes	enzyme
T9477	14695-14697	,	denotes	,
T9478	14697-14701	IN	denotes	with
T9479	14702-14710	JJ	denotes	elevated
T9480	14711-14717	NNS	denotes	levels
T9481	14718-14720	IN	denotes	of
T9482	14721-14727	JJ	denotes	single
T9484	14727-14728	HYPH	denotes	-
T9483	14728-14733	NN	denotes	chain
T9486	14734-14740	JJ	denotes	mature
T9485	14741-14747	NN	denotes	enzyme
T9487	14747-14748	.	denotes	.
T2850	14749-14752	DT	denotes	The
T2849	14749-14768	sentence	denotes	The impact of the a
T2851	14753-14754	NN	denotes	i
T2852	14754-14755	JJ	denotes	m
T2853	14755-14756	NN	denotes	p
T2854	14756-14757	CC	denotes	a
T2855	14757-14758	JJ	denotes	c
T2856	14758-14759	NNS	denotes	t
T2857	14759-14760	,	denotes
T2858	14760-14761	JJR	denotes	o
T2859	14761-14762	IN	denotes	f
T2860	14763-14764	JJ	denotes	t
T2861	14764-14765	NN	denotes	h
T2862	14765-14766	JJ	denotes	e
T2863	14767-14768	NN	denotes	a
T2865	14768-14769	RB	denotes	l
T2864	14768-14780	sentence	denotes	ltered cathe
T2868	14769-14770	NN	denotes	t
T2867	14770-14772	NNS	denotes	er
T2866	14772-14774	VBD	denotes	ed
T2869	14775-14776	VBN	denotes	c
T2870	14776-14777	CC	denotes	a
T2871	14777-14780	DT	denotes	the
T2873	14780-14781	NN	denotes	p
T2872	14780-14923	sentence	denotes	psin D processing on enzymatic activity was next tested to determine if altered enzymatic activity accounts for inefficient subunit c turnover.
T2876	14781-14784	NN	denotes	sin
T2877	14785-14786	NN	denotes	D
T2875	14787-14797	NN	denotes	processing
T2878	14798-14800	IN	denotes	on
T2879	14801-14810	JJ	denotes	enzymatic
T2880	14811-14819	NN	denotes	activity
T2881	14820-14823	VBD	denotes	was
T2882	14824-14828	RB	denotes	next
T2874	14829-14835	VBN	denotes	tested
T2883	14836-14838	TO	denotes	to
T2884	14839-14848	VB	denotes	determine
T2885	14849-14851	IN	denotes	if
T2887	14852-14859	VBN	denotes	altered
T2889	14860-14869	JJ	denotes	enzymatic
T2888	14870-14878	NN	denotes	activity
T2886	14879-14887	VBZ	denotes	accounts
T2890	14888-14891	IN	denotes	for
T2891	14892-14903	JJ	denotes	inefficient
T2893	14904-14911	NN	denotes	subunit
T2894	14912-14913	NN	denotes	c
T2892	14914-14922	NN	denotes	turnover
T2895	14922-14923	.	denotes	.
T2896	14923-15251	sentence	denotes	In a fluorogenic in vitro assay, cathepsin D activity in total cellular extracts was not significantly altered in homozygous CbCln3Δex7/8 cells (376 ± 89 RFU/μg total protein), versus wild-type cells (324 ± 58 RFU/μg total protein), although a consistent trend towards increased enzymatic activity in mutant cells was observed.
T2897	14924-14926	IN	denotes	In
T2899	14927-14928	DT	denotes	a
T2901	14929-14940	JJ	denotes	fluorogenic
T2902	14941-14943	FW	denotes	in
T2903	14944-14949	FW	denotes	vitro
T2900	14950-14955	NN	denotes	assay
T2904	14955-14957	,	denotes	,
T2905	14957-14966	NN	denotes	cathepsin
T2906	14967-14968	NN	denotes	D
T2907	14969-14977	NN	denotes	activity
T2908	14978-14980	IN	denotes	in
T2909	14981-14986	JJ	denotes	total
T2911	14987-14995	JJ	denotes	cellular
T2910	14996-15004	NNS	denotes	extracts
T2912	15005-15008	VBD	denotes	was
T2913	15009-15012	RB	denotes	not
T2914	15013-15026	RB	denotes	significantly
T2898	15027-15034	VBN	denotes	altered
T2915	15035-15037	IN	denotes	in
T2916	15038-15048	JJ	denotes	homozygous
T2918	15049-15059	NN	denotes	CbCln3Δex7
T2919	15059-15060	HYPH	denotes	/
T2920	15060-15061	CD	denotes	8
T2917	15062-15067	NNS	denotes	cells
T2921	15068-15069	-LRB-	denotes	(
T2923	15069-15072	CD	denotes	376
T2925	15073-15074	SYM	denotes	±
T2924	15075-15077	CD	denotes	89
T2922	15078-15081	NN	denotes	RFU
T2926	15081-15082	SYM	denotes	/
T2928	15082-15084	NN	denotes	μg
T2929	15085-15090	JJ	denotes	total
T2927	15091-15098	NN	denotes	protein
T2930	15098-15099	-RRB-	denotes	)
T2931	15099-15101	,	denotes	,
T2932	15101-15107	CC	denotes	versus
T2933	15108-15112	JJ	denotes	wild
T2935	15112-15113	HYPH	denotes	-
T2934	15113-15117	NN	denotes	type
T2936	15118-15123	NNS	denotes	cells
T2937	15124-15125	-LRB-	denotes	(
T2939	15125-15128	CD	denotes	324
T2941	15129-15130	IN	denotes	±
T2940	15131-15133	CD	denotes	58
T2938	15134-15137	NN	denotes	RFU
T2942	15137-15138	SYM	denotes	/
T2944	15138-15140	NN	denotes	μg
T2945	15141-15146	JJ	denotes	total
T2943	15147-15154	NN	denotes	protein
T2946	15154-15155	-RRB-	denotes	)
T2947	15155-15157	,	denotes	,
T2948	15157-15165	IN	denotes	although
T2950	15166-15167	DT	denotes	a
T2952	15168-15178	JJ	denotes	consistent
T2951	15179-15184	NN	denotes	trend
T2953	15185-15192	IN	denotes	towards
T2954	15193-15202	VBN	denotes	increased
T2956	15203-15212	JJ	denotes	enzymatic
T2955	15213-15221	NN	denotes	activity
T2957	15222-15224	IN	denotes	in
T2958	15225-15231	NN	denotes	mutant
T2959	15232-15237	NNS	denotes	cells
T2960	15238-15241	VBD	denotes	was
T2949	15242-15250	VBN	denotes	observed
T2961	15250-15251	.	denotes	.
T2962	15251-15419	sentence	denotes	Thus, cathepsin D transport and processing are disrupted in homozygous CbCln3Δex7/8 cells in a manner such that enzymatic activity appears to be relatively unaffected.
T2963	15252-15256	RB	denotes	Thus
T2965	15256-15258	,	denotes	,
T2966	15258-15267	NN	denotes	cathepsin
T2967	15268-15269	NN	denotes	D
T2968	15270-15279	NN	denotes	transport
T2969	15280-15283	CC	denotes	and
T2970	15284-15294	NN	denotes	processing
T2971	15295-15298	VBP	denotes	are
T2964	15299-15308	VBN	denotes	disrupted
T2972	15309-15311	IN	denotes	in
T2973	15312-15322	JJ	denotes	homozygous
T2975	15323-15333	NN	denotes	CbCln3Δex7
T2976	15333-15334	HYPH	denotes	/
T2977	15334-15335	CD	denotes	8
T2974	15336-15341	NNS	denotes	cells
T2978	15342-15344	IN	denotes	in
T2979	15345-15346	DT	denotes	a
T2980	15347-15353	NN	denotes	manner
T2981	15354-15358	JJ	denotes	such
T2982	15359-15363	IN	denotes	that
T2984	15364-15373	JJ	denotes	enzymatic
T2985	15374-15382	NN	denotes	activity
T2983	15383-15390	VBZ	denotes	appears
T2986	15391-15393	TO	denotes	to
T2987	15394-15396	VB	denotes	be
T2988	15397-15407	RB	denotes	relatively
T2989	15408-15418	JJ	denotes	unaffected
T2990	15418-15419	.	denotes	.
T3349	15421-15431	JJ	denotes	Homozygous
T3351	15432-15442	NN	denotes	CbCln3Δex7
T3352	15442-15443	HYPH	denotes	/
T3353	15443-15444	CD	denotes	8
T3350	15445-15450	NNS	denotes	cells
T3354	15451-15455	VBP	denotes	show
T3355	15456-15464	JJ	denotes	abnormal
T3357	15465-15473	NN	denotes	membrane
T3356	15474-15484	NNS	denotes	organelles
T3358	15484-15705	sentence	denotes	The abnormal transport and processing of cathepsin D suggested membrane trafficking disruptions in homozygous CbCln3Δex7/8 cells; therefore, we surveyed the subcellular distribution and morphology of membrane organelles.
T3359	15485-15488	DT	denotes	The
T3361	15489-15497	JJ	denotes	abnormal
T3360	15498-15507	NN	denotes	transport
T3363	15508-15511	CC	denotes	and
T3364	15512-15522	NN	denotes	processing
T3365	15523-15525	IN	denotes	of
T3366	15526-15535	NN	denotes	cathepsin
T3367	15536-15537	NN	denotes	D
T3362	15538-15547	VBD	denotes	suggested
T3369	15548-15556	NN	denotes	membrane
T3370	15557-15568	NN	denotes	trafficking
T3371	15569-15580	NNS	denotes	disruptions
T3372	15581-15583	IN	denotes	in
T3373	15584-15594	JJ	denotes	homozygous
T3375	15595-15605	NN	denotes	CbCln3Δex7
T3376	15605-15606	HYPH	denotes	/
T3377	15606-15607	CD	denotes	8
T3374	15608-15613	NNS	denotes	cells
T3378	15613-15614	:	denotes	;
T3379	15615-15624	RB	denotes	therefore
T3380	15624-15626	,	denotes	,
T3381	15626-15628	PRP	denotes	we
T3368	15629-15637	VBD	denotes	surveyed
T3382	15638-15641	DT	denotes	the
T3384	15642-15653	JJ	denotes	subcellular
T3383	15654-15666	NN	denotes	distribution
T3385	15667-15670	CC	denotes	and
T3386	15671-15681	NN	denotes	morphology
T3387	15682-15684	IN	denotes	of
T3388	15685-15693	NN	denotes	membrane
T3389	15694-15704	NNS	denotes	organelles
T3390	15704-15705	.	denotes	.
T3391	15705-15947	sentence	denotes	Components of the secretory pathway, including the ER, cis-Golgi, and trans-Golgi, did not appear altered from wild-type appearance when labeled with the respective markers, protein disulfide isomerase (PDI), GM130, and VVL (data not shown).
T3392	15706-15716	NNS	denotes	Components
T3394	15717-15719	IN	denotes	of
T3395	15720-15723	DT	denotes	the
T3397	15724-15733	JJ	denotes	secretory
T3396	15734-15741	NN	denotes	pathway
T3398	15741-15743	,	denotes	,
T3399	15743-15752	VBG	denotes	including
T3400	15753-15756	DT	denotes	the
T3401	15757-15759	NN	denotes	ER
T3402	15759-15761	,	denotes	,
T3403	15761-15764	NN	denotes	cis
T3405	15764-15765	HYPH	denotes	-
T3404	15765-15770	NN	denotes	Golgi
T3406	15770-15772	,	denotes	,
T3407	15772-15775	CC	denotes	and
T3408	15776-15781	NN	denotes	trans
T3410	15781-15782	HYPH	denotes	-
T3409	15782-15787	NN	denotes	Golgi
T3411	15787-15789	,	denotes	,
T3412	15789-15792	VBD	denotes	did
T3413	15793-15796	RB	denotes	not
T3393	15797-15803	VB	denotes	appear
T3414	15804-15811	JJ	denotes	altered
T3415	15812-15816	IN	denotes	from
T3416	15817-15821	JJ	denotes	wild
T3418	15821-15822	HYPH	denotes	-
T3417	15822-15826	NN	denotes	type
T3419	15827-15837	NN	denotes	appearance
T3420	15838-15842	WRB	denotes	when
T3421	15843-15850	VBN	denotes	labeled
T3422	15851-15855	IN	denotes	with
T3423	15856-15859	DT	denotes	the
T3425	15860-15870	JJ	denotes	respective
T3424	15871-15878	NNS	denotes	markers
T3426	15878-15880	,	denotes	,
T3427	15880-15887	NN	denotes	protein
T3429	15888-15897	NN	denotes	disulfide
T3428	15898-15907	NN	denotes	isomerase
T3430	15908-15909	-LRB-	denotes	(
T3431	15909-15912	NN	denotes	PDI
T3432	15912-15913	-RRB-	denotes	)
T3433	15913-15915	,	denotes	,
T3434	15915-15920	NN	denotes	GM130
T3435	15920-15922	,	denotes	,
T3436	15922-15925	CC	denotes	and
T3437	15926-15929	NN	denotes	VVL
T3438	15930-15931	-LRB-	denotes	(
T3440	15931-15935	NNS	denotes	data
T3441	15936-15939	RB	denotes	not
T3439	15940-15945	VBN	denotes	shown
T3442	15945-15946	-RRB-	denotes	)
T3443	15946-15947	.	denotes	.
T3444	15947-16097	sentence	denotes	By contrast, the lysosomal markers, Lysotracker and Lamp 2 had significantly altered signal in homozygous CbCln3Δex7/8 cells, versus wild-type cells.
T3445	15948-15950	IN	denotes	By
T3447	15951-15959	NN	denotes	contrast
T3448	15959-15961	,	denotes	,
T3449	15961-15964	DT	denotes	the
T3451	15965-15974	JJ	denotes	lysosomal
T3450	15975-15982	NNS	denotes	markers
T3452	15982-15984	,	denotes	,
T3453	15984-15995	NN	denotes	Lysotracker
T3454	15996-15999	CC	denotes	and
T3455	16000-16004	NN	denotes	Lamp
T3456	16005-16006	CD	denotes	2
T3446	16007-16010	VBD	denotes	had
T3457	16011-16024	RB	denotes	significantly
T3458	16025-16032	JJ	denotes	altered
T3459	16033-16039	NN	denotes	signal
T3460	16040-16042	IN	denotes	in
T3461	16043-16053	JJ	denotes	homozygous
T3463	16054-16064	NN	denotes	CbCln3Δex7
T3464	16064-16065	HYPH	denotes	/
T3465	16065-16066	CD	denotes	8
T3462	16067-16072	NNS	denotes	cells
T3466	16072-16074	,	denotes	,
T3467	16074-16080	CC	denotes	versus
T3468	16081-16085	JJ	denotes	wild
T3470	16085-16086	HYPH	denotes	-
T3469	16086-16090	NN	denotes	type
T3471	16091-16096	NNS	denotes	cells
T3472	16096-16097	.	denotes	.
T3473	16097-16361	sentence	denotes	Wild-type cells exhibited brightly stained lysosomes that were large and clustered in the perinuclear region whereas homozygous CbCln3Δex7/8 lysosomes were lightly stained, smaller vesicles that were more diffusely scattered in the cytoplasm of the cell (Fig. 6).
T3474	16098-16102	JJ	denotes	Wild
T3476	16102-16103	HYPH	denotes	-
T3475	16103-16107	NN	denotes	type
T3477	16108-16113	NNS	denotes	cells
T3478	16114-16123	VBD	denotes	exhibited
T3479	16124-16132	RB	denotes	brightly
T3480	16133-16140	VBN	denotes	stained
T3481	16141-16150	NNS	denotes	lysosomes
T3482	16151-16155	WDT	denotes	that
T3483	16156-16160	VBD	denotes	were
T3484	16161-16166	JJ	denotes	large
T3485	16167-16170	CC	denotes	and
T3486	16171-16180	JJ	denotes	clustered
T3487	16181-16183	IN	denotes	in
T3488	16184-16187	DT	denotes	the
T3490	16188-16199	JJ	denotes	perinuclear
T3489	16200-16206	NN	denotes	region
T3491	16207-16214	IN	denotes	whereas
T3493	16215-16225	JJ	denotes	homozygous
T3495	16226-16236	NN	denotes	CbCln3Δex7
T3496	16236-16237	HYPH	denotes	/
T3497	16237-16238	CD	denotes	8
T3494	16239-16248	NNS	denotes	lysosomes
T3492	16249-16253	VBD	denotes	were
T3498	16254-16261	RB	denotes	lightly
T3499	16262-16269	VBN	denotes	stained
T3501	16269-16271	,	denotes	,
T3502	16271-16278	JJR	denotes	smaller
T3500	16279-16287	NNS	denotes	vesicles
T3503	16288-16292	WDT	denotes	that
T3505	16293-16297	VBD	denotes	were
T3506	16298-16302	RBR	denotes	more
T3507	16303-16312	RB	denotes	diffusely
T3504	16313-16322	VBN	denotes	scattered
T3508	16323-16325	IN	denotes	in
T3509	16326-16329	DT	denotes	the
T3510	16330-16339	NN	denotes	cytoplasm
T3511	16340-16342	IN	denotes	of
T3512	16343-16346	DT	denotes	the
T3513	16347-16351	NN	denotes	cell
T3514	16352-16353	-LRB-	denotes	(
T3515	16353-16357	NN	denotes	Fig.
T3516	16358-16359	CD	denotes	6
T3517	16359-16360	-RRB-	denotes	)
T3518	16360-16361	.	denotes	.
T3519	16361-16429	sentence	denotes	Lamp 1 distribution was also altered, as previously noted (Fig. 3).
T3520	16362-16366	NN	denotes	Lamp
T3522	16367-16368	CD	denotes	1
T3521	16369-16381	NN	denotes	distribution
T3524	16382-16385	VBD	denotes	was
T3525	16386-16390	RB	denotes	also
T3523	16391-16398	VBN	denotes	altered
T3526	16398-16400	,	denotes	,
T3527	16400-16402	IN	denotes	as
T3529	16403-16413	RB	denotes	previously
T3528	16414-16419	VBN	denotes	noted
T3530	16420-16421	-LRB-	denotes	(
T3531	16421-16425	NN	denotes	Fig.
T3532	16426-16427	CD	denotes	3
T3533	16427-16428	-RRB-	denotes	)
T3534	16428-16429	.	denotes	.
T3535	16429-16697	sentence	denotes	However, Lamp 1 and Lamp 2 total protein levels were similar in wild-type and homozygous CbCln3Δex7/8 cells by immunoblot analysis, indicating the altered signal likely reflects dispersed lysosomes or altered localization and/or epitope availability (data not shown).
T3536	16430-16437	RB	denotes	However
T3538	16437-16439	,	denotes	,
T3539	16439-16443	NN	denotes	Lamp
T3541	16444-16445	CD	denotes	1
T3542	16446-16449	CC	denotes	and
T3543	16450-16454	NN	denotes	Lamp
T3544	16455-16456	CD	denotes	2
T3545	16457-16462	JJ	denotes	total
T3546	16463-16470	NN	denotes	protein
T3540	16471-16477	NNS	denotes	levels
T3537	16478-16482	VBD	denotes	were
T3547	16483-16490	JJ	denotes	similar
T3548	16491-16493	IN	denotes	in
T3549	16494-16498	JJ	denotes	wild
T3551	16498-16499	HYPH	denotes	-
T3550	16499-16503	NN	denotes	type
T3553	16504-16507	CC	denotes	and
T3554	16508-16518	JJ	denotes	homozygous
T3555	16519-16529	NN	denotes	CbCln3Δex7
T3556	16529-16530	HYPH	denotes	/
T3557	16530-16531	CD	denotes	8
T3552	16532-16537	NNS	denotes	cells
T3558	16538-16540	IN	denotes	by
T3559	16541-16551	NN	denotes	immunoblot
T3560	16552-16560	NN	denotes	analysis
T3561	16560-16562	,	denotes	,
T3562	16562-16572	VBG	denotes	indicating
T3563	16573-16576	DT	denotes	the
T3565	16577-16584	JJ	denotes	altered
T3564	16585-16591	NN	denotes	signal
T3567	16592-16598	RB	denotes	likely
T3566	16599-16607	VBZ	denotes	reflects
T3568	16608-16617	JJ	denotes	dispersed
T3569	16618-16627	NNS	denotes	lysosomes
T3570	16628-16630	CC	denotes	or
T3571	16631-16638	JJ	denotes	altered
T3572	16639-16651	NN	denotes	localization
T3573	16652-16655	CC	denotes	and
T3574	16655-16656	HYPH	denotes	/
T3575	16656-16658	CC	denotes	or
T3576	16659-16666	NN	denotes	epitope
T3577	16667-16679	NN	denotes	availability
T3578	16680-16681	-LRB-	denotes	(
T3580	16681-16685	NNS	denotes	data
T3581	16686-16689	RB	denotes	not
T3579	16690-16695	VBN	denotes	shown
T3582	16695-16696	-RRB-	denotes	)
T3583	16696-16697	.	denotes	.
T3584	16697-16849	sentence	denotes	It is noteworthy that Lysotracker dye, which selectively accumulates in acidic compartments, exhibited the most marked reduction in lysosomal labeling.
T3585	16698-16700	PRP	denotes	It
T3586	16701-16703	VBZ	denotes	is
T3587	16704-16714	JJ	denotes	noteworthy
T3588	16715-16719	IN	denotes	that
T3590	16720-16731	NN	denotes	Lysotracker
T3591	16732-16735	NN	denotes	dye
T3592	16735-16737	,	denotes	,
T3593	16737-16742	WDT	denotes	which
T3595	16743-16754	RB	denotes	selectively
T3594	16755-16766	VBZ	denotes	accumulates
T3596	16767-16769	IN	denotes	in
T3597	16770-16776	JJ	denotes	acidic
T3598	16777-16789	NNS	denotes	compartments
T3599	16789-16791	,	denotes	,
T3589	16791-16800	VBD	denotes	exhibited
T3600	16801-16804	DT	denotes	the
T3602	16805-16809	RBS	denotes	most
T3603	16810-16816	JJ	denotes	marked
T3601	16817-16826	NN	denotes	reduction
T3604	16827-16829	IN	denotes	in
T3605	16830-16839	JJ	denotes	lysosomal
T3606	16840-16848	NN	denotes	labeling
T3607	16848-16849	.	denotes	.
T3608	16849-16940	sentence	denotes	This observation may reflect altered lysosomal pH, an established finding in JNCL [10,15].
T3609	16850-16854	DT	denotes	This
T3610	16855-16866	NN	denotes	observation
T3612	16867-16870	MD	denotes	may
T3611	16871-16878	VB	denotes	reflect
T3613	16879-16886	JJ	denotes	altered
T3615	16887-16896	JJ	denotes	lysosomal
T3614	16897-16899	NN	denotes	pH
T3616	16899-16901	,	denotes	,
T3617	16901-16903	DT	denotes	an
T3619	16904-16915	JJ	denotes	established
T3618	16916-16923	NN	denotes	finding
T3620	16924-16926	IN	denotes	in
T3621	16927-16931	NN	denotes	JNCL
T3622	16932-16933	-LRB-	denotes	[
T3624	16933-16935	CD	denotes	10
T3625	16935-16936	,	denotes	,
T3623	16936-16938	CD	denotes	15
T3626	16938-16939	-RRB-	denotes	]
T3627	16939-16940	.	denotes	.
T3628	16940-16941	sentence	denotes
T9581	16951-16962	NN	denotes	Lysotracker
T9583	16963-16966	CC	denotes	and
T9584	16967-16971	NN	denotes	Lamp
T9585	16972-16973	CD	denotes	2
T9582	16974-16982	NN	denotes	labeling
T9587	16983-16985	IN	denotes	of
T9588	16986-16990	JJ	denotes	wild
T9590	16990-16991	HYPH	denotes	-
T9589	16991-16995	NN	denotes	type
T9592	16996-16999	CC	denotes	and
T9593	17000-17010	JJ	denotes	homozygous
T9594	17011-17021	NN	denotes	CbCln3Δex7
T9595	17021-17022	HYPH	denotes	/
T9596	17022-17023	CD	denotes	8
T9591	17024-17033	NNS	denotes	lysosomes
T9597	17034-17043	JJ	denotes	Lysosomal
T9598	17044-17052	NN	denotes	labeling
T9599	17053-17055	IN	denotes	of
T9600	17056-17060	JJ	denotes	wild
T9602	17060-17061	HYPH	denotes	-
T9601	17061-17065	NN	denotes	type
T9604	17066-17069	CC	denotes	and
T9605	17070-17080	JJ	denotes	homozygous
T9606	17081-17091	NN	denotes	CbCln3Δex7
T9607	17091-17092	HYPH	denotes	/
T9608	17092-17093	CD	denotes	8
T9609	17094-17103	NN	denotes	precursor
T9603	17104-17109	NNS	denotes	cells
T9610	17110-17114	IN	denotes	with
T9611	17115-17126	NN	denotes	lysotracker
T9613	17127-17130	CC	denotes	and
T9614	17131-17135	NN	denotes	Lamp
T9615	17136-17137	CD	denotes	2
T9612	17138-17146	NN	denotes	antibody
T9616	17147-17149	VBZ	denotes	is
T9586	17150-17155	VBN	denotes	shown
T9617	17155-17156	.	denotes	.
T9618	17156-17305	sentence	denotes	Lysotracker dye (top panels) labeled large, perinuclear-clustered lysosomes and scattered lysosomes in the periphery of wild-type cells (CbCln3+/+).
T9619	17157-17168	NN	denotes	Lysotracker
T9620	17169-17172	NN	denotes	dye
T9622	17173-17174	-LRB-	denotes	(
T9624	17174-17177	JJ	denotes	top
T9623	17178-17184	NNS	denotes	panels
T9625	17184-17185	-RRB-	denotes	)
T9621	17186-17193	VBN	denotes	labeled
T9626	17194-17199	JJ	denotes	large
T9628	17199-17201	,	denotes	,
T9629	17201-17212	JJ	denotes	perinuclear
T9631	17212-17213	HYPH	denotes	-
T9630	17213-17222	VBN	denotes	clustered
T9627	17223-17232	NNS	denotes	lysosomes
T9632	17233-17236	CC	denotes	and
T9633	17237-17246	VBN	denotes	scattered
T9634	17247-17256	NNS	denotes	lysosomes
T9635	17257-17259	IN	denotes	in
T9636	17260-17263	DT	denotes	the
T9637	17264-17273	NN	denotes	periphery
T9638	17274-17276	IN	denotes	of
T9639	17277-17281	JJ	denotes	wild
T9641	17281-17282	HYPH	denotes	-
T9640	17282-17286	NN	denotes	type
T9642	17287-17292	NNS	denotes	cells
T9643	17293-17294	-LRB-	denotes	(
T9644	17294-17300	NN	denotes	CbCln3
T9645	17300-17301	SYM	denotes	+
T9646	17301-17302	HYPH	denotes	/
T9647	17302-17303	SYM	denotes	+
T9648	17303-17304	-RRB-	denotes	)
T9649	17304-17305	.	denotes	.
T9650	17305-17470	sentence	denotes	Lysotracker stain was dramatically reduced in homozygous mutant cells (CbCln3Δex7/8/Δex7/8), with smaller labeled vesicles and less apparent perinuclear clustering.
T9651	17306-17317	NN	denotes	Lysotracker
T9652	17318-17323	NN	denotes	stain
T9653	17324-17327	VBD	denotes	was
T9654	17328-17340	RB	denotes	dramatically
T9655	17341-17348	JJ	denotes	reduced
T9656	17349-17351	IN	denotes	in
T9657	17352-17362	JJ	denotes	homozygous
T9659	17363-17369	NN	denotes	mutant
T9658	17370-17375	NNS	denotes	cells
T9660	17376-17377	-LRB-	denotes	(
T9661	17377-17387	NN	denotes	CbCln3Δex7
T9662	17387-17388	HYPH	denotes	/
T9663	17388-17389	CD	denotes	8
T9664	17389-17390	HYPH	denotes	/
T9665	17390-17394	NN	denotes	Δex7
T9666	17394-17395	HYPH	denotes	/
T9667	17395-17396	CD	denotes	8
T9668	17396-17397	-RRB-	denotes	)
T9669	17397-17399	,	denotes	,
T9670	17399-17403	IN	denotes	with
T9671	17404-17411	JJR	denotes	smaller
T9673	17412-17419	VBN	denotes	labeled
T9672	17420-17428	NNS	denotes	vesicles
T9674	17429-17432	CC	denotes	and
T9675	17433-17437	RBR	denotes	less
T9676	17438-17446	JJ	denotes	apparent
T9678	17447-17458	JJ	denotes	perinuclear
T9677	17459-17469	NN	denotes	clustering
T9679	17469-17470	.	denotes	.
T9680	17470-17700	sentence	denotes	Lamp 2 (bottom panels) immunostaining also showed reduced signal intensity with less perinuclear clustering in homozygous CbCln3Δex7/8 cells, although the effect was somewhat less dramatic than that observed with Lysotracker dye.
T9681	17471-17475	NN	denotes	Lamp
T9683	17476-17477	CD	denotes	2
T9684	17478-17479	-LRB-	denotes	(
T9686	17479-17485	JJ	denotes	bottom
T9685	17486-17492	NNS	denotes	panels
T9687	17492-17493	-RRB-	denotes	)
T9682	17494-17508	NN	denotes	immunostaining
T9689	17509-17513	RB	denotes	also
T9688	17514-17520	VBD	denotes	showed
T9690	17521-17528	VBN	denotes	reduced
T9692	17529-17535	NN	denotes	signal
T9691	17536-17545	NN	denotes	intensity
T9693	17546-17550	IN	denotes	with
T9694	17551-17555	RBR	denotes	less
T9696	17556-17567	JJ	denotes	perinuclear
T9695	17568-17578	NN	denotes	clustering
T9697	17579-17581	IN	denotes	in
T9698	17582-17592	JJ	denotes	homozygous
T9700	17593-17603	NN	denotes	CbCln3Δex7
T9701	17603-17604	HYPH	denotes	/
T9702	17604-17605	CD	denotes	8
T9699	17606-17611	NNS	denotes	cells
T9703	17611-17613	,	denotes	,
T9704	17613-17621	IN	denotes	although
T9706	17622-17625	DT	denotes	the
T9707	17626-17632	NN	denotes	effect
T9705	17633-17636	VBD	denotes	was
T9708	17637-17645	RB	denotes	somewhat
T9709	17646-17650	RBR	denotes	less
T9710	17651-17659	JJ	denotes	dramatic
T9711	17660-17664	IN	denotes	than
T9712	17665-17669	DT	denotes	that
T9713	17670-17678	VBN	denotes	observed
T9714	17679-17683	IN	denotes	with
T9715	17684-17695	NN	denotes	Lysotracker
T9716	17696-17699	NN	denotes	dye
T9717	17699-17700	.	denotes	.
T9718	17700-17802	sentence	denotes	Wild-type and homozygous CbCln3Δex7/8 confocal images were captured with identical exposure settings.
T9719	17701-17705	JJ	denotes	Wild
T9721	17705-17706	HYPH	denotes	-
T9720	17706-17710	NN	denotes	type
T9723	17711-17714	CC	denotes	and
T9724	17715-17725	JJ	denotes	homozygous
T9725	17726-17736	NN	denotes	CbCln3Δex7
T9726	17736-17737	HYPH	denotes	/
T9727	17737-17738	CD	denotes	8
T9728	17739-17747	JJ	denotes	confocal
T9722	17748-17754	NNS	denotes	images
T9730	17755-17759	VBD	denotes	were
T9729	17760-17768	VBN	denotes	captured
T9731	17769-17773	IN	denotes	with
T9732	17774-17783	JJ	denotes	identical
T9734	17784-17792	NN	denotes	exposure
T9733	17793-17801	NNS	denotes	settings
T9735	17801-17802	.	denotes	.
T9736	17802-17822	sentence	denotes	60 × magnification.
T9737	17803-17805	CD	denotes	60
T9739	17806-17807	SYM	denotes	×
T9738	17808-17821	NN	denotes	magnification
T9740	17821-17822	.	denotes	.
T3632	17823-17824	NN	denotes	C
T3629	17823-17835	sentence	denotes	Consistent w
T3631	17824-17825	NNS	denotes	o
T3633	17825-17826	NN	denotes	n
T3634	17826-17827	JJ	denotes	s
T3635	17827-17828	IN	denotes	i
T3636	17828-17831	NN	denotes	ste
T3638	17831-17832	CC	denotes	n
T3637	17832-17833	NN	denotes	t
T3630	17834-17835	VBN	denotes	w
T3640	17835-17836	JJ	denotes	i
T3639	17835-17840	sentence	denotes	ith t
T3641	17836-17837	VBN	denotes	t
T3642	17837-17838	DT	denotes	h
T3643	17839-17840	NN	denotes	t
T3645	17840-17841	JJ	denotes	h
T3644	17840-17849	sentence	denotes	he altere
T3646	17841-17842	NNS	denotes	e
T3647	17843-17845	JJR	denotes	al
T3648	17845-17846	JJ	denotes	t
T3649	17846-17849	JJ	denotes	ere
T3651	17849-17850	VBD	denotes	d
T3650	17849-17861	sentence	denotes	d early endo
T3652	17851-17852	VBN	denotes	e
T3653	17852-17853	NN	denotes	a
T3654	17853-17855	JJ	denotes	rl
T3655	17855-17856	JJ	denotes	y
T3656	17857-17858	NN	denotes	e
T3657	17858-17859	IN	denotes	n
T3658	17859-17860	VBN	denotes	d
T3659	17860-17861	NN	denotes	o
T3662	17861-17862	JJ	denotes	s
T3660	17861-17866	sentence	denotes	some
T3663	17862-17863	JJ	denotes	o
T3661	17863-17865	NNS	denotes	me
T3664	17866-17868	sentence	denotes	ma
T3665	17866-17868	NN	denotes	ma
T3669	17868-17869	JJ	denotes	r
T3666	17868-18043	sentence	denotes	rker (EEA1) signal observed by immunostaining (Fig. 3), fluid-phase endocytosis was also altered in homozygous CbCln3Δex7/8 cells, as measured by dextran-FITC uptake (Fig. 7).
T3670	17869-17872	NN	denotes	ker
T3671	17873-17874	-LRB-	denotes	(
T3672	17874-17878	NN	denotes	EEA1
T3673	17878-17879	-RRB-	denotes	)
T3668	17880-17886	NN	denotes	signal
T3674	17887-17895	VBN	denotes	observed
T3675	17896-17898	IN	denotes	by
T3676	17899-17913	NN	denotes	immunostaining
T3677	17914-17915	-LRB-	denotes	(
T3678	17915-17919	NN	denotes	Fig.
T3679	17920-17921	CD	denotes	3
T3680	17921-17922	-RRB-	denotes	)
T3681	17922-17924	,	denotes	,
T3682	17924-17929	NN	denotes	fluid
T3684	17929-17930	HYPH	denotes	-
T3683	17930-17935	NN	denotes	phase
T3685	17936-17947	NN	denotes	endocytosis
T3686	17948-17951	VBD	denotes	was
T3687	17952-17956	RB	denotes	also
T3667	17957-17964	VBN	denotes	altered
T3688	17965-17967	IN	denotes	in
T3689	17968-17978	JJ	denotes	homozygous
T3691	17979-17989	NN	denotes	CbCln3Δex7
T3692	17989-17990	HYPH	denotes	/
T3693	17990-17991	CD	denotes	8
T3690	17992-17997	NNS	denotes	cells
T3694	17997-17999	,	denotes	,
T3695	17999-18001	IN	denotes	as
T3696	18002-18010	VBN	denotes	measured
T3697	18011-18013	IN	denotes	by
T3698	18014-18021	NN	denotes	dextran
T3700	18021-18022	HYPH	denotes	-
T3699	18022-18026	NN	denotes	FITC
T3701	18027-18033	NN	denotes	uptake
T3702	18034-18035	-LRB-	denotes	(
T3703	18035-18039	NN	denotes	Fig.
T3704	18040-18041	CD	denotes	7
T3705	18041-18042	-RRB-	denotes	)
T3706	18042-18043	.	denotes	.
T3707	18043-18243	sentence	denotes	Following a 15-minute incubation in media containing dextran-FITC, wild-type and heterozygote cells displayed brightly stained, large endocytic vesicles that were clustered in the perinuclear region.
T3708	18044-18053	VBG	denotes	Following
T3710	18054-18055	DT	denotes	a
T3712	18056-18058	CD	denotes	15
T3714	18058-18059	HYPH	denotes	-
T3713	18059-18065	NN	denotes	minute
T3711	18066-18076	NN	denotes	incubation
T3715	18077-18079	IN	denotes	in
T3716	18080-18085	NNS	denotes	media
T3717	18086-18096	VBG	denotes	containing
T3718	18097-18104	NN	denotes	dextran
T3720	18104-18105	HYPH	denotes	-
T3719	18105-18109	NN	denotes	FITC
T3721	18109-18111	,	denotes	,
T3722	18111-18115	JJ	denotes	wild
T3724	18115-18116	HYPH	denotes	-
T3723	18116-18120	NN	denotes	type
T3726	18121-18124	CC	denotes	and
T3727	18125-18137	NN	denotes	heterozygote
T3725	18138-18143	NNS	denotes	cells
T3709	18144-18153	VBD	denotes	displayed
T3728	18154-18162	RB	denotes	brightly
T3729	18163-18170	VBN	denotes	stained
T3731	18170-18172	,	denotes	,
T3732	18172-18177	JJ	denotes	large
T3733	18178-18187	JJ	denotes	endocytic
T3730	18188-18196	NNS	denotes	vesicles
T3734	18197-18201	WDT	denotes	that
T3736	18202-18206	VBD	denotes	were
T3735	18207-18216	VBN	denotes	clustered
T3737	18217-18219	IN	denotes	in
T3738	18220-18223	DT	denotes	the
T3740	18224-18235	JJ	denotes	perinuclear
T3739	18236-18242	NN	denotes	region
T3741	18242-18243	.	denotes	.
T3742	18243-18418	sentence	denotes	However, homozygous CbCln3Δex7/8 cells were less brightly stained with most dextran-FITC signal localizing to smaller vesicles scattered throughout the cytoplasm of the cell.
T3743	18244-18251	RB	denotes	However
T3745	18251-18253	,	denotes	,
T3746	18253-18263	JJ	denotes	homozygous
T3748	18264-18274	NN	denotes	CbCln3Δex7
T3749	18274-18275	HYPH	denotes	/
T3750	18275-18276	CD	denotes	8
T3747	18277-18282	NNS	denotes	cells
T3751	18283-18287	VBD	denotes	were
T3752	18288-18292	RBR	denotes	less
T3753	18293-18301	RB	denotes	brightly
T3744	18302-18309	VBN	denotes	stained
T3754	18310-18314	IN	denotes	with
T3755	18315-18319	JJS	denotes	most
T3757	18320-18327	NN	denotes	dextran
T3759	18327-18328	HYPH	denotes	-
T3758	18328-18332	NN	denotes	FITC
T3756	18333-18339	NN	denotes	signal
T3760	18340-18350	VBG	denotes	localizing
T3761	18351-18353	IN	denotes	to
T3762	18354-18361	JJR	denotes	smaller
T3763	18362-18370	NNS	denotes	vesicles
T3764	18371-18380	VBN	denotes	scattered
T3765	18381-18391	IN	denotes	throughout
T3766	18392-18395	DT	denotes	the
T3767	18396-18405	NN	denotes	cytoplasm
T3768	18406-18408	IN	denotes	of
T3769	18409-18412	DT	denotes	the
T3770	18413-18417	NN	denotes	cell
T3771	18417-18418	.	denotes	.
T3772	18418-18421	sentence	denotes	Fi
T3773	18419-18421	NN	denotes	Fi
T9816	18429-18440	NN	denotes	Endocytosis
T9818	18441-18443	IN	denotes	in
T9819	18444-18448	JJ	denotes	wild
T9821	18448-18449	HYPH	denotes	-
T9820	18449-18453	NN	denotes	type
T9823	18453-18455	,	denotes	,
T9824	18455-18467	JJ	denotes	heterozygous
T9825	18468-18471	CC	denotes	and
T9826	18472-18482	JJ	denotes	homozygous
T9827	18483-18493	NN	denotes	CbCln3Δex7
T9828	18493-18494	HYPH	denotes	/
T9829	18494-18495	CD	denotes	8
T9822	18496-18501	NNS	denotes	cells
T9830	18502-18509	NN	denotes	Dextran
T9832	18509-18510	HYPH	denotes	-
T9831	18510-18514	NN	denotes	FITC
T9833	18515-18521	NN	denotes	uptake
T9834	18522-18524	IN	denotes	in
T9835	18525-18529	JJ	denotes	wild
T9837	18529-18530	HYPH	denotes	-
T9836	18530-18534	NN	denotes	type
T9839	18534-18536	,	denotes	,
T9840	18536-18548	JJ	denotes	heterozygous
T9841	18549-18552	CC	denotes	and
T9842	18553-18563	JJ	denotes	homozygous
T9843	18564-18574	NN	denotes	CbCln3Δex7
T9844	18574-18575	HYPH	denotes	/
T9845	18575-18576	CD	denotes	8
T9846	18577-18586	NN	denotes	precursor
T9838	18587-18592	NNS	denotes	cells
T9847	18593-18595	VBZ	denotes	is
T9817	18596-18601	VBN	denotes	shown
T9848	18601-18602	.	denotes	.
T9849	18602-18834	sentence	denotes	In wild-type (CbCln3+/+, left panel) and heterozygous (CbCln3+/Δex7/8, middle panel) cells, dextran-FITC label was observed in a perinuclear-clustered vesicular pattern with scattered labeled vesicles also present in the periphery.
T9850	18603-18605	IN	denotes	In
T9852	18606-18610	JJ	denotes	wild
T9854	18610-18611	HYPH	denotes	-
T9853	18611-18615	NN	denotes	type
T9856	18616-18617	-LRB-	denotes	(
T9857	18617-18623	NN	denotes	CbCln3
T9858	18623-18624	SYM	denotes	+
T9859	18624-18625	HYPH	denotes	/
T9860	18625-18626	SYM	denotes	+
T9861	18626-18628	,	denotes	,
T9863	18628-18632	JJ	denotes	left
T9862	18633-18638	NN	denotes	panel
T9864	18638-18639	-RRB-	denotes	)
T9865	18640-18643	CC	denotes	and
T9866	18644-18656	JJ	denotes	heterozygous
T9867	18657-18658	-LRB-	denotes	(
T9869	18658-18664	NN	denotes	CbCln3
T9871	18664-18665	SYM	denotes	+
T9872	18665-18666	HYPH	denotes	/
T9870	18666-18670	NN	denotes	Δex7
T9873	18670-18671	HYPH	denotes	/
T9874	18671-18672	CD	denotes	8
T9875	18672-18674	,	denotes	,
T9876	18674-18680	JJ	denotes	middle
T9868	18681-18686	NN	denotes	panel
T9877	18686-18687	-RRB-	denotes	)
T9855	18688-18693	NNS	denotes	cells
T9878	18693-18695	,	denotes	,
T9879	18695-18702	NN	denotes	dextran
T9881	18702-18703	HYPH	denotes	-
T9880	18703-18707	NN	denotes	FITC
T9882	18708-18713	NN	denotes	label
T9883	18714-18717	VBD	denotes	was
T9851	18718-18726	VBN	denotes	observed
T9884	18727-18729	IN	denotes	in
T9885	18730-18731	DT	denotes	a
T9887	18732-18743	JJ	denotes	perinuclear
T9889	18743-18744	HYPH	denotes	-
T9888	18744-18753	VBN	denotes	clustered
T9890	18754-18763	JJ	denotes	vesicular
T9886	18764-18771	NN	denotes	pattern
T9891	18772-18776	IN	denotes	with
T9892	18777-18786	VBN	denotes	scattered
T9894	18787-18794	VBN	denotes	labeled
T9893	18795-18803	NNS	denotes	vesicles
T9895	18804-18808	RB	denotes	also
T9896	18809-18816	JJ	denotes	present
T9897	18817-18819	IN	denotes	in
T9898	18820-18823	DT	denotes	the
T9899	18824-18833	NN	denotes	periphery
T9900	18833-18834	.	denotes	.
T9901	18834-19021	sentence	denotes	In contrast, dextran-FITC label of homozygous mutant (CbCln3Δex7/8/Δex7/8, right panel) cells was reduced overall and exhibited smaller stained vesicles with less perinuclear clustering.
T9902	18835-18837	IN	denotes	In
T9904	18838-18846	NN	denotes	contrast
T9905	18846-18848	,	denotes	,
T9906	18848-18855	NN	denotes	dextran
T9908	18855-18856	HYPH	denotes	-
T9907	18856-18860	NN	denotes	FITC
T9909	18861-18866	NN	denotes	label
T9910	18867-18869	IN	denotes	of
T9911	18870-18880	JJ	denotes	homozygous
T9912	18881-18887	NN	denotes	mutant
T9914	18888-18889	-LRB-	denotes	(
T9915	18889-18899	NN	denotes	CbCln3Δex7
T9917	18899-18900	HYPH	denotes	/
T9918	18900-18901	CD	denotes	8
T9919	18901-18902	HYPH	denotes	/
T9916	18902-18906	NN	denotes	Δex7
T9920	18906-18907	HYPH	denotes	/
T9921	18907-18908	CD	denotes	8
T9922	18908-18910	,	denotes	,
T9924	18910-18915	JJ	denotes	right
T9923	18916-18921	NN	denotes	panel
T9925	18921-18922	-RRB-	denotes	)
T9913	18923-18928	NNS	denotes	cells
T9926	18929-18932	VBD	denotes	was
T9903	18933-18940	VBN	denotes	reduced
T9927	18941-18948	RB	denotes	overall
T9928	18949-18952	CC	denotes	and
T9929	18953-18962	VBD	denotes	exhibited
T9930	18963-18970	JJR	denotes	smaller
T9932	18971-18978	VBN	denotes	stained
T9931	18979-18987	NNS	denotes	vesicles
T9933	18988-18992	IN	denotes	with
T9934	18993-18997	RBR	denotes	less
T9936	18998-19009	JJ	denotes	perinuclear
T9935	19010-19020	NN	denotes	clustering
T9937	19020-19021	.	denotes	.
T9938	19021-19085	sentence	denotes	Confocal images were captured with identical exposure settings.
T9939	19022-19030	JJ	denotes	Confocal
T9940	19031-19037	NNS	denotes	images
T9942	19038-19042	VBD	denotes	were
T9941	19043-19051	VBN	denotes	captured
T9943	19052-19056	IN	denotes	with
T9944	19057-19066	JJ	denotes	identical
T9946	19067-19075	NN	denotes	exposure
T9945	19076-19084	NNS	denotes	settings
T9947	19084-19085	.	denotes	.
T9948	19085-19105	sentence	denotes	40 × magnification.
T9949	19086-19088	CD	denotes	40
T9951	19089-19090	SYM	denotes	×
T9950	19091-19104	NN	denotes	magnification
T9952	19104-19105	.	denotes	.
T3775	19108-19109	NN	denotes	n
T3774	19108-19118	sentence	denotes	nally, bec
T3777	19109-19110	CC	denotes	a
T3778	19110-19111	NN	denotes	l
T3776	19111-19112	NNS	denotes	l
T3779	19112-19113	NN	denotes	y
T3780	19113-19115	,	denotes	,
T3781	19115-19117	NN	denotes	be
T3782	19117-19118	NN	denotes	c
T3785	19118-19119	NN	denotes	a
T3783	19118-19126	sentence	denotes	ause sub
T3786	19119-19121	JJ	denotes	us
T3787	19121-19122	NN	denotes	e
T3788	19122-19123	,	denotes
T3784	19123-19124	NNS	denotes	s
T3789	19124-19125	JJ	denotes	u
T3790	19125-19126	VBN	denotes	b
T3792	19126-19127	JJ	denotes	u
T3791	19126-19138	sentence	denotes	unit c is a
T3793	19127-19128	NN	denotes	n
T3795	19128-19130	JJ	denotes	it
T3794	19131-19132	NNS	denotes	c
T3796	19133-19134	VBD	denotes	i
T3797	19134-19135	JJR	denotes	s
T3798	19136-19137	VBN	denotes	a
T3800	19138-19139	NNS	denotes	m
T3799	19138-19142	sentence	denotes	mito
T3801	19139-19141	IN	denotes	it
T3802	19141-19142	NN	denotes	o
T3803	19142-19143	sentence	denotes	c
T3804	19142-19143	NN	denotes	c
T3808	19143-19151	JJ	denotes	hondrial
T3805	19143-19317	sentence	denotes	hondrial protein and its turnover proceeds through autophagic engulfment of mitochondria [13], we analyzed homozygous CbCln3Δex7/8 cell mitochondrial morphology and function.
T3807	19152-19159	NN	denotes	protein
T3809	19160-19163	CC	denotes	and
T3810	19164-19167	PRP$	denotes	its
T3811	19168-19176	NN	denotes	turnover
T3812	19177-19185	VBZ	denotes	proceeds
T3813	19186-19193	IN	denotes	through
T3814	19194-19204	JJ	denotes	autophagic
T3815	19205-19215	NN	denotes	engulfment
T3816	19216-19218	IN	denotes	of
T3817	19219-19231	NNS	denotes	mitochondria
T3818	19232-19233	-LRB-	denotes	[
T3819	19233-19235	CD	denotes	13
T3820	19235-19236	-RRB-	denotes	]
T3821	19236-19238	,	denotes	,
T3822	19238-19240	PRP	denotes	we
T3806	19241-19249	VBD	denotes	analyzed
T3823	19250-19260	JJ	denotes	homozygous
T3824	19261-19271	NN	denotes	CbCln3Δex7
T3825	19271-19272	HYPH	denotes	/
T3826	19272-19273	CD	denotes	8
T3827	19274-19278	NN	denotes	cell
T3828	19279-19292	JJ	denotes	mitochondrial
T3829	19293-19303	NN	denotes	morphology
T3830	19304-19307	CC	denotes	and
T3831	19308-19316	NN	denotes	function
T3832	19316-19317	.	denotes	.
T3833	19317-19567	sentence	denotes	Mitochondrial distribution in homozygous CbCln3Δex7/8 cells was indistinguishable from wild-type and heterozygous cells; however, homozygous CbCln3Δex7/8 mitochondria appeared more elongated by grp75 marker immunostaining and TEM analysis (Fig. 8a).
T3834	19318-19331	JJ	denotes	Mitochondrial
T3835	19332-19344	NN	denotes	distribution
T3837	19345-19347	IN	denotes	in
T3838	19348-19358	JJ	denotes	homozygous
T3840	19359-19369	NN	denotes	CbCln3Δex7
T3841	19369-19370	HYPH	denotes	/
T3842	19370-19371	CD	denotes	8
T3839	19372-19377	NNS	denotes	cells
T3836	19378-19381	VBD	denotes	was
T3844	19382-19399	JJ	denotes	indistinguishable
T3845	19400-19404	IN	denotes	from
T3846	19405-19409	JJ	denotes	wild
T3848	19409-19410	HYPH	denotes	-
T3847	19410-19414	NN	denotes	type
T3850	19415-19418	CC	denotes	and
T3851	19419-19431	JJ	denotes	heterozygous
T3849	19432-19437	NNS	denotes	cells
T3852	19437-19438	:	denotes	;
T3853	19439-19446	RB	denotes	however
T3854	19446-19448	,	denotes	,
T3855	19448-19458	JJ	denotes	homozygous
T3857	19459-19469	NN	denotes	CbCln3Δex7
T3858	19469-19470	HYPH	denotes	/
T3859	19470-19471	CD	denotes	8
T3856	19472-19484	NNS	denotes	mitochondria
T3843	19485-19493	VBD	denotes	appeared
T3860	19494-19498	RBR	denotes	more
T3861	19499-19508	JJ	denotes	elongated
T3862	19509-19511	IN	denotes	by
T3863	19512-19517	NN	denotes	grp75
T3865	19518-19524	NN	denotes	marker
T3864	19525-19539	NN	denotes	immunostaining
T3866	19540-19543	CC	denotes	and
T3867	19544-19547	NN	denotes	TEM
T3868	19548-19556	NN	denotes	analysis
T3869	19557-19558	-LRB-	denotes	(
T3871	19558-19562	NN	denotes	Fig.
T3870	19563-19565	NN	denotes	8a
T3872	19565-19566	-RRB-	denotes	)
T3873	19566-19567	.	denotes	.
T3874	19567-19761	sentence	denotes	72% of homozygous mutant mitochondria were greater than 0.6 μm in length (range = 0.26 μm to 2.75 μm), while fewer wild-type mitochondria (51%) reached this length (range = 0.15 μm to 2.29 μm).
T3875	19568-19570	CD	denotes	72
T3876	19570-19571	NN	denotes	%
T3878	19572-19574	IN	denotes	of
T3879	19575-19585	JJ	denotes	homozygous
T3881	19586-19592	NN	denotes	mutant
T3880	19593-19605	NNS	denotes	mitochondria
T3877	19606-19610	VBD	denotes	were
T3882	19611-19618	JJR	denotes	greater
T3884	19619-19623	IN	denotes	than
T3883	19624-19627	CD	denotes	0.6
T3885	19628-19630	NN	denotes	μm
T3886	19631-19633	IN	denotes	in
T3887	19634-19640	NN	denotes	length
T3888	19641-19642	-LRB-	denotes	(
T3890	19642-19647	NN	denotes	range
T3891	19648-19649	SYM	denotes	=
T3892	19650-19654	CD	denotes	0.26
T3889	19655-19657	NN	denotes	μm
T3893	19658-19660	IN	denotes	to
T3894	19661-19665	CD	denotes	2.75
T3895	19666-19668	NN	denotes	μm
T3896	19668-19669	-RRB-	denotes	)
T3897	19669-19671	,	denotes	,
T3898	19671-19676	IN	denotes	while
T3900	19677-19682	JJR	denotes	fewer
T3902	19683-19687	JJ	denotes	wild
T3904	19687-19688	HYPH	denotes	-
T3903	19688-19692	NN	denotes	type
T3901	19693-19705	NNS	denotes	mitochondria
T3905	19706-19707	-LRB-	denotes	(
T3907	19707-19709	CD	denotes	51
T3906	19709-19710	NN	denotes	%
T3908	19710-19711	-RRB-	denotes	)
T3899	19712-19719	VBD	denotes	reached
T3909	19720-19724	DT	denotes	this
T3910	19725-19731	NN	denotes	length
T3911	19732-19733	-LRB-	denotes	(
T3913	19733-19738	NN	denotes	range
T3914	19739-19740	SYM	denotes	=
T3915	19741-19745	CD	denotes	0.15
T3912	19746-19748	NN	denotes	μm
T3916	19749-19751	IN	denotes	to
T3917	19752-19756	CD	denotes	2.29
T3918	19757-19759	NN	denotes	μm
T3919	19759-19760	-RRB-	denotes	)
T3920	19760-19761	.	denotes	.
T3921	19761-19848	sentence	denotes	Mitochondrial width was not altered in homozygous CbCln3Δex7/8 cells (data not shown).
T3922	19762-19775	JJ	denotes	Mitochondrial
T3923	19776-19781	NN	denotes	width
T3925	19782-19785	VBD	denotes	was
T3926	19786-19789	RB	denotes	not
T3924	19790-19797	VBN	denotes	altered
T3927	19798-19800	IN	denotes	in
T3928	19801-19811	JJ	denotes	homozygous
T3930	19812-19822	NN	denotes	CbCln3Δex7
T3931	19822-19823	HYPH	denotes	/
T3932	19823-19824	CD	denotes	8
T3929	19825-19830	NNS	denotes	cells
T3933	19831-19832	-LRB-	denotes	(
T3935	19832-19836	NNS	denotes	data
T3936	19837-19840	RB	denotes	not
T3934	19841-19846	VBN	denotes	shown
T3937	19846-19847	-RRB-	denotes	)
T3938	19847-19848	.	denotes	.
T3939	19848-20181	sentence	denotes	Moreover, compared to wild-type or heterozygous cells, homozygous CbCln3Δex7/8 cells had significantly reduced cellular ATP levels (1.3 fold less, Fig. 8b) and exhibited reduced survival following hydrogen peroxide treatment (~50% of wild-type survival, Fig. 8c), suggesting impaired energy metabolism and oxidative stress response.
T3940	19849-19857	RB	denotes	Moreover
T3942	19857-19859	,	denotes	,
T3943	19859-19867	VBN	denotes	compared
T3944	19868-19870	IN	denotes	to
T3945	19871-19875	JJ	denotes	wild
T3947	19875-19876	HYPH	denotes	-
T3946	19876-19880	NN	denotes	type
T3949	19881-19883	CC	denotes	or
T3950	19884-19896	JJ	denotes	heterozygous
T3948	19897-19902	NNS	denotes	cells
T3951	19902-19904	,	denotes	,
T3952	19904-19914	JJ	denotes	homozygous
T3954	19915-19925	NN	denotes	CbCln3Δex7
T3955	19925-19926	HYPH	denotes	/
T3956	19926-19927	CD	denotes	8
T3953	19928-19933	NNS	denotes	cells
T3941	19934-19937	VBD	denotes	had
T3957	19938-19951	RB	denotes	significantly
T3958	19952-19959	VBN	denotes	reduced
T3960	19960-19968	JJ	denotes	cellular
T3961	19969-19972	NN	denotes	ATP
T3959	19973-19979	NNS	denotes	levels
T3962	19980-19981	-LRB-	denotes	(
T3964	19981-19984	CD	denotes	1.3
T3966	19985-19989	RB	denotes	fold
T3965	19990-19994	JJR	denotes	less
T3967	19994-19996	,	denotes	,
T3963	19996-20000	NN	denotes	Fig.
T3968	20001-20003	CD	denotes	8b
T3969	20003-20004	-RRB-	denotes	)
T3970	20005-20008	CC	denotes	and
T3971	20009-20018	VBD	denotes	exhibited
T3972	20019-20026	VBN	denotes	reduced
T3973	20027-20035	NN	denotes	survival
T3974	20036-20045	VBG	denotes	following
T3975	20046-20054	NN	denotes	hydrogen
T3976	20055-20063	NN	denotes	peroxide
T3977	20064-20073	NN	denotes	treatment
T3978	20074-20075	-LRB-	denotes	(
T3980	20075-20076	SYM	denotes	~
T3981	20076-20078	CD	denotes	50
T3982	20078-20079	NN	denotes	%
T3983	20080-20082	IN	denotes	of
T3984	20083-20087	JJ	denotes	wild
T3986	20087-20088	HYPH	denotes	-
T3985	20088-20092	NN	denotes	type
T3987	20093-20101	NN	denotes	survival
T3988	20101-20103	,	denotes	,
T3989	20103-20107	NN	denotes	Fig.
T3979	20108-20110	NN	denotes	8c
T3990	20110-20111	-RRB-	denotes	)
T3991	20111-20113	,	denotes	,
T3992	20113-20123	VBG	denotes	suggesting
T3993	20124-20132	JJ	denotes	impaired
T3995	20133-20139	NN	denotes	energy
T3994	20140-20150	NN	denotes	metabolism
T3996	20151-20154	CC	denotes	and
T3997	20155-20164	JJ	denotes	oxidative
T3999	20165-20171	NN	denotes	stress
T3998	20172-20180	NN	denotes	response
T4000	20180-20181	.	denotes	.
T4001	20181-20282	sentence	denotes	Taken together, these data support impaired mitochondrial function in homozygous CbCln3Δex7/8 cells.
T4002	20182-20187	VBN	denotes	Taken
T4004	20188-20196	RB	denotes	together
T4005	20196-20198	,	denotes	,
T4006	20198-20203	DT	denotes	these
T4007	20204-20208	NNS	denotes	data
T4003	20209-20216	VBP	denotes	support
T4008	20217-20225	JJ	denotes	impaired
T4010	20226-20239	JJ	denotes	mitochondrial
T4009	20240-20248	NN	denotes	function
T4011	20249-20251	IN	denotes	in
T4012	20252-20262	JJ	denotes	homozygous
T4014	20263-20273	NN	denotes	CbCln3Δex7
T4015	20273-20274	HYPH	denotes	/
T4016	20274-20275	CD	denotes	8
T4013	20276-20281	NNS	denotes	cells
T4017	20281-20282	.	denotes	.
T10102	20293-20306	JJ	denotes	Mitochondrial
T10103	20307-20317	NN	denotes	morphology
T10104	20318-20321	CC	denotes	and
T10105	20322-20330	NN	denotes	function
T10106	20331-20333	IN	denotes	in
T10107	20334-20338	JJ	denotes	wild
T10109	20338-20339	HYPH	denotes	-
T10108	20339-20343	NN	denotes	type
T10111	20343-20345	,	denotes	,
T10112	20345-20357	JJ	denotes	heterozygous
T10113	20358-20361	CC	denotes	and
T10114	20362-20372	JJ	denotes	homozygous
T10115	20373-20383	NN	denotes	CbCln3Δex7
T10116	20383-20384	HYPH	denotes	/
T10117	20384-20385	CD	denotes	8
T10110	20386-20391	NNS	denotes	cells
T10118	20391-20500	sentence	denotes	a. Confocal and TEM micrographs of wild-type and homozygous CbCln3Δex7/8 mitochondrial morphology are shown.
T10119	20392-20393	LS	denotes	a
T10121	20393-20394	.	denotes	.
T10122	20395-20403	JJ	denotes	Confocal
T10124	20404-20407	CC	denotes	and
T10125	20408-20411	NN	denotes	TEM
T10123	20412-20423	NNS	denotes	micrographs
T10126	20424-20426	IN	denotes	of
T10127	20427-20431	JJ	denotes	wild
T10129	20431-20432	HYPH	denotes	-
T10128	20432-20436	NN	denotes	type
T10131	20437-20440	CC	denotes	and
T10132	20441-20451	JJ	denotes	homozygous
T10133	20452-20462	NN	denotes	CbCln3Δex7
T10134	20462-20463	HYPH	denotes	/
T10135	20463-20464	CD	denotes	8
T10136	20465-20478	JJ	denotes	mitochondrial
T10130	20479-20489	NN	denotes	morphology
T10137	20490-20493	VBP	denotes	are
T10120	20494-20499	VBN	denotes	shown
T10138	20499-20500	.	denotes	.
T10139	20500-20736	sentence	denotes	Immunostaining with the inner mitochondrial membrane marker, grp75 (top panels) highlighted elongated mitochondria in homozygous mutant cells (CbCln3Δex7/8/Δex7/8), relative to wild-type mitochondria (CbCln3+/+) (insets, zoom = 2.75x).
T10140	20501-20515	NN	denotes	Immunostaining
T10142	20516-20520	IN	denotes	with
T10143	20521-20524	DT	denotes	the
T10145	20525-20530	JJ	denotes	inner
T10146	20531-20544	JJ	denotes	mitochondrial
T10147	20545-20553	NN	denotes	membrane
T10144	20554-20560	NN	denotes	marker
T10148	20560-20562	,	denotes	,
T10149	20562-20567	NN	denotes	grp75
T10150	20568-20569	-LRB-	denotes	(
T10152	20569-20572	JJ	denotes	top
T10151	20573-20579	NNS	denotes	panels
T10153	20579-20580	-RRB-	denotes	)
T10141	20581-20592	VBD	denotes	highlighted
T10154	20593-20602	VBN	denotes	elongated
T10155	20603-20615	NNS	denotes	mitochondria
T10156	20616-20618	IN	denotes	in
T10157	20619-20629	JJ	denotes	homozygous
T10159	20630-20636	NN	denotes	mutant
T10158	20637-20642	NNS	denotes	cells
T10160	20643-20644	-LRB-	denotes	(
T10161	20644-20654	NN	denotes	CbCln3Δex7
T10163	20654-20655	HYPH	denotes	/
T10164	20655-20656	CD	denotes	8
T10165	20656-20657	HYPH	denotes	/
T10162	20657-20661	NN	denotes	Δex7
T10166	20661-20662	HYPH	denotes	/
T10167	20662-20663	CD	denotes	8
T10168	20663-20664	-RRB-	denotes	)
T10169	20664-20666	,	denotes	,
T10170	20666-20674	JJ	denotes	relative
T10171	20675-20677	IN	denotes	to
T10172	20678-20682	JJ	denotes	wild
T10174	20682-20683	HYPH	denotes	-
T10173	20683-20687	NN	denotes	type
T10175	20688-20700	NNS	denotes	mitochondria
T10176	20701-20702	-LRB-	denotes	(
T10177	20702-20708	NN	denotes	CbCln3
T10178	20708-20709	SYM	denotes	+
T10179	20709-20710	HYPH	denotes	/
T10180	20710-20711	SYM	denotes	+
T10181	20711-20712	-RRB-	denotes	)
T10182	20713-20714	-LRB-	denotes	(
T10183	20714-20720	NNS	denotes	insets
T10184	20720-20722	,	denotes	,
T10185	20722-20726	NN	denotes	zoom
T10187	20727-20728	SYM	denotes	=
T10186	20729-20733	CD	denotes	2.75
T10188	20733-20734	SYM	denotes	x
T10189	20734-20735	-RRB-	denotes	)
T10190	20735-20736	.	denotes	.
T10191	20736-20807	sentence	denotes	Mitochondrial distribution was not altered from the wild-type pattern.
T10192	20737-20750	JJ	denotes	Mitochondrial
T10193	20751-20763	NN	denotes	distribution
T10195	20764-20767	VBD	denotes	was
T10196	20768-20771	RB	denotes	not
T10194	20772-20779	VBN	denotes	altered
T10197	20780-20784	IN	denotes	from
T10198	20785-20788	DT	denotes	the
T10200	20789-20793	JJ	denotes	wild
T10202	20793-20794	HYPH	denotes	-
T10201	20794-20798	NN	denotes	type
T10199	20799-20806	NN	denotes	pattern
T10203	20806-20807	.	denotes	.
T10204	20807-20890	sentence	denotes	Elongated homozygous CbCln3Δex7/8 mitochondria were also observed by TEM analysis.
T10205	20808-20817	VBN	denotes	Elongated
T10207	20818-20828	JJ	denotes	homozygous
T10208	20829-20839	NN	denotes	CbCln3Δex7
T10209	20839-20840	HYPH	denotes	/
T10210	20840-20841	CD	denotes	8
T10206	20842-20854	NNS	denotes	mitochondria
T10212	20855-20859	VBD	denotes	were
T10213	20860-20864	RB	denotes	also
T10211	20865-20873	VBN	denotes	observed
T10214	20874-20876	IN	denotes	by
T10215	20877-20880	NN	denotes	TEM
T10216	20881-20889	NN	denotes	analysis
T10217	20889-20890	.	denotes	.
T10218	20890-20910	sentence	denotes	60 × magnification.
T10219	20891-20893	CD	denotes	60
T10221	20894-20895	SYM	denotes	×
T10220	20896-20909	NN	denotes	magnification
T10222	20909-20910	.	denotes	.
T10223	20910-21015	sentence	denotes	b. Cellular ATP levels in wild-type, heterozygous and homozygous CbCln3Δex7/8 precursor cells are shown.
T10224	20911-20912	LS	denotes	b
T10226	20912-20913	.	denotes	.
T10227	20914-20922	JJ	denotes	Cellular
T10228	20923-20926	NN	denotes	ATP
T10229	20927-20933	NNS	denotes	levels
T10230	20934-20936	IN	denotes	in
T10231	20937-20941	JJ	denotes	wild
T10233	20941-20942	HYPH	denotes	-
T10232	20942-20946	NN	denotes	type
T10235	20946-20948	,	denotes	,
T10236	20948-20960	JJ	denotes	heterozygous
T10237	20961-20964	CC	denotes	and
T10238	20965-20975	JJ	denotes	homozygous
T10239	20976-20986	NN	denotes	CbCln3Δex7
T10240	20986-20987	HYPH	denotes	/
T10241	20987-20988	CD	denotes	8
T10242	20989-20998	NN	denotes	precursor
T10234	20999-21004	NNS	denotes	cells
T10243	21005-21008	VBP	denotes	are
T10225	21009-21014	VBN	denotes	shown
T10244	21014-21015	.	denotes	.
T10245	21015-21267	sentence	denotes	Wild-type (open bar) and heterozygous (gray bar) CbCln3Δex7/8 cells contained ~39 μM ATP, while homozygous CbCln3Δex7/8 cells (black bar) contained ~1.3 fold reduced levels of ATP (~30 μM), which was statistically significant in a t-test (p < 0.0001).
T10246	21016-21020	JJ	denotes	Wild
T10248	21020-21021	HYPH	denotes	-
T10247	21021-21025	NN	denotes	type
T10250	21026-21027	-LRB-	denotes	(
T10252	21027-21031	JJ	denotes	open
T10251	21032-21035	NN	denotes	bar
T10253	21035-21036	-RRB-	denotes	)
T10254	21037-21040	CC	denotes	and
T10255	21041-21053	JJ	denotes	heterozygous
T10256	21054-21055	-LRB-	denotes	(
T10258	21055-21059	JJ	denotes	gray
T10257	21060-21063	NN	denotes	bar
T10259	21063-21064	-RRB-	denotes	)
T10260	21065-21075	NN	denotes	CbCln3Δex7
T10261	21075-21076	HYPH	denotes	/
T10262	21076-21077	CD	denotes	8
T10249	21078-21083	NNS	denotes	cells
T10263	21084-21093	VBD	denotes	contained
T10264	21094-21095	SYM	denotes	~
T10265	21095-21097	CD	denotes	39
T10266	21098-21100	NN	denotes	μM
T10267	21101-21104	NN	denotes	ATP
T10268	21104-21106	,	denotes	,
T10269	21106-21111	IN	denotes	while
T10271	21112-21122	JJ	denotes	homozygous
T10273	21123-21133	NN	denotes	CbCln3Δex7
T10274	21133-21134	HYPH	denotes	/
T10275	21134-21135	CD	denotes	8
T10272	21136-21141	NNS	denotes	cells
T10276	21142-21143	-LRB-	denotes	(
T10278	21143-21148	JJ	denotes	black
T10277	21149-21152	NN	denotes	bar
T10279	21152-21153	-RRB-	denotes	)
T10270	21154-21163	VBD	denotes	contained
T10280	21164-21165	SYM	denotes	~
T10281	21165-21168	CD	denotes	1.3
T10283	21169-21173	RB	denotes	fold
T10282	21174-21181	VBN	denotes	reduced
T10284	21182-21188	NNS	denotes	levels
T10285	21189-21191	IN	denotes	of
T10286	21192-21195	NN	denotes	ATP
T10287	21196-21197	-LRB-	denotes	(
T10288	21197-21198	SYM	denotes	~
T10289	21198-21200	CD	denotes	30
T10290	21201-21203	NN	denotes	μM
T10291	21203-21204	-RRB-	denotes	)
T10292	21204-21206	,	denotes	,
T10293	21206-21211	WDT	denotes	which
T10294	21212-21215	VBD	denotes	was
T10295	21216-21229	RB	denotes	statistically
T10296	21230-21241	JJ	denotes	significant
T10297	21242-21244	IN	denotes	in
T10298	21245-21246	DT	denotes	a
T10300	21247-21248	NN	denotes	t
T10301	21248-21249	HYPH	denotes	-
T10299	21249-21253	NN	denotes	test
T10302	21254-21255	-LRB-	denotes	(
T10304	21255-21256	NN	denotes	p
T10305	21257-21258	SYM	denotes	<
T10303	21259-21265	CD	denotes	0.0001
T10306	21265-21266	-RRB-	denotes	)
T10307	21266-21267	.	denotes	.
T10308	21267-21387	sentence	denotes	Wild-type and heterozygous CbCln3Δex7/8 cellular ATP levels were not statistically different from each other (p > 0.4).
T10309	21268-21272	JJ	denotes	Wild
T10311	21272-21273	HYPH	denotes	-
T10310	21273-21277	NN	denotes	type
T10313	21278-21281	CC	denotes	and
T10314	21282-21294	JJ	denotes	heterozygous
T10315	21295-21305	NN	denotes	CbCln3Δex7
T10317	21305-21306	HYPH	denotes	/
T10316	21306-21307	NN	denotes	8
T10318	21308-21316	JJ	denotes	cellular
T10319	21317-21320	NN	denotes	ATP
T10312	21321-21327	NNS	denotes	levels
T10320	21328-21332	VBD	denotes	were
T10321	21333-21336	RB	denotes	not
T10322	21337-21350	RB	denotes	statistically
T10323	21351-21360	JJ	denotes	different
T10324	21361-21365	IN	denotes	from
T10325	21366-21370	DT	denotes	each
T10326	21371-21376	JJ	denotes	other
T10327	21377-21378	-LRB-	denotes	(
T10329	21378-21379	NN	denotes	p
T10330	21380-21381	SYM	denotes	>
T10328	21382-21385	CD	denotes	0.4
T10331	21385-21386	-RRB-	denotes	)
T10332	21386-21387	.	denotes	.
T10333	21387-21467	sentence	denotes	A representative of triplicate experiments is shown (n = 6 in each experiment).
T10334	21388-21389	DT	denotes	A
T10335	21390-21404	NN	denotes	representative
T10337	21405-21407	IN	denotes	of
T10338	21408-21418	JJ	denotes	triplicate
T10339	21419-21430	NNS	denotes	experiments
T10340	21431-21433	VBZ	denotes	is
T10336	21434-21439	VBN	denotes	shown
T10341	21440-21441	-LRB-	denotes	(
T10343	21441-21442	NN	denotes	n
T10344	21443-21444	SYM	denotes	=
T10342	21445-21446	CD	denotes	6
T10345	21447-21449	IN	denotes	in
T10346	21450-21454	DT	denotes	each
T10347	21455-21465	NN	denotes	experiment
T10348	21465-21466	-RRB-	denotes	)
T10349	21466-21467	.	denotes	.
T10350	21467-21540	sentence	denotes	c. Cell survival following 24-hour hydrogen peroxide treatment is shown.
T10351	21468-21469	LS	denotes	c
T10353	21469-21470	.	denotes	.
T10354	21471-21475	NN	denotes	Cell
T10355	21476-21484	NN	denotes	survival
T10356	21485-21494	VBG	denotes	following
T10357	21495-21497	CD	denotes	24
T10359	21497-21498	HYPH	denotes	-
T10358	21498-21502	NN	denotes	hour
T10361	21503-21511	NN	denotes	hydrogen
T10362	21512-21520	NN	denotes	peroxide
T10360	21521-21530	NN	denotes	treatment
T10363	21531-21533	VBZ	denotes	is
T10352	21534-21539	VBN	denotes	shown
T10364	21539-21540	.	denotes	.
T10365	21540-21650	sentence	denotes	Homozygous CbCln3Δex7/8 cells were ~2-fold more sensitive to oxidative stress by hydrogen peroxide treatment.
T10366	21541-21551	JJ	denotes	Homozygous
T10368	21552-21562	NN	denotes	CbCln3Δex7
T10369	21562-21563	HYPH	denotes	/
T10370	21563-21564	CD	denotes	8
T10367	21565-21570	NNS	denotes	cells
T10371	21571-21575	VBD	denotes	were
T10372	21576-21577	SYM	denotes	~
T10373	21577-21583	RB	denotes	2-fold
T10374	21584-21588	RBR	denotes	more
T10375	21589-21598	JJ	denotes	sensitive
T10376	21599-21601	IN	denotes	to
T10377	21602-21611	JJ	denotes	oxidative
T10378	21612-21618	NN	denotes	stress
T10379	21619-21621	IN	denotes	by
T10380	21622-21630	NN	denotes	hydrogen
T10381	21631-21639	NN	denotes	peroxide
T10382	21640-21649	NN	denotes	treatment
T10383	21649-21650	.	denotes	.
T10384	21650-21857	sentence	denotes	Wild-type (circle) and heterozygous (triangle) CbCln3Δex7/8 cells exhibited ~50% survival rates with 75–100 μM H2O2, whereas homozygous CbCln3Δex7/8 cells (squares) had a ~50% survival rate with 50 μM H2O2.
T10385	21651-21655	JJ	denotes	Wild
T10387	21655-21656	HYPH	denotes	-
T10386	21656-21660	NN	denotes	type
T10389	21661-21662	-LRB-	denotes	(
T10390	21662-21668	NN	denotes	circle
T10391	21668-21669	-RRB-	denotes	)
T10392	21670-21673	CC	denotes	and
T10393	21674-21686	JJ	denotes	heterozygous
T10394	21687-21688	-LRB-	denotes	(
T10395	21688-21696	NN	denotes	triangle
T10396	21696-21697	-RRB-	denotes	)
T10397	21698-21708	NN	denotes	CbCln3Δex7
T10398	21708-21709	HYPH	denotes	/
T10399	21709-21710	CD	denotes	8
T10388	21711-21716	NNS	denotes	cells
T10400	21717-21726	VBD	denotes	exhibited
T10401	21727-21728	SYM	denotes	~
T10402	21728-21730	CD	denotes	50
T10403	21730-21731	NN	denotes	%
T10405	21732-21740	NN	denotes	survival
T10404	21741-21746	NNS	denotes	rates
T10406	21747-21751	IN	denotes	with
T10407	21752-21754	CD	denotes	75
T10409	21754-21755	SYM	denotes	–
T10408	21755-21758	CD	denotes	100
T10410	21759-21761	NN	denotes	μM
T10411	21762-21766	NN	denotes	H2O2
T10412	21766-21768	,	denotes	,
T10413	21768-21775	IN	denotes	whereas
T10415	21776-21786	JJ	denotes	homozygous
T10417	21787-21797	NN	denotes	CbCln3Δex7
T10418	21797-21798	HYPH	denotes	/
T10419	21798-21799	CD	denotes	8
T10416	21800-21805	NNS	denotes	cells
T10420	21806-21807	-LRB-	denotes	(
T10421	21807-21814	NNS	denotes	squares
T10422	21814-21815	-RRB-	denotes	)
T10414	21816-21819	VBD	denotes	had
T10423	21820-21821	DT	denotes	a
T10425	21822-21823	SYM	denotes	~
T10426	21823-21825	CD	denotes	50
T10427	21825-21826	NN	denotes	%
T10428	21827-21835	NN	denotes	survival
T10424	21836-21840	NN	denotes	rate
T10429	21841-21845	IN	denotes	with
T10430	21846-21848	CD	denotes	50
T10431	21849-21851	NN	denotes	μM
T10432	21852-21856	NN	denotes	H2O2
T10433	21856-21857	.	denotes	.
T10434	21857-21937	sentence	denotes	A representative of triplicate experiments is shown (n = 4 in each experiment).
T10435	21858-21859	DT	denotes	A
T10436	21860-21874	NN	denotes	representative
T10438	21875-21877	IN	denotes	of
T10439	21878-21888	JJ	denotes	triplicate
T10440	21889-21900	NNS	denotes	experiments
T10441	21901-21903	VBZ	denotes	is
T10437	21904-21909	VBN	denotes	shown
T10442	21910-21911	-LRB-	denotes	(
T10444	21911-21912	NN	denotes	n
T10445	21913-21914	SYM	denotes	=
T10443	21915-21916	CD	denotes	4
T10446	21917-21919	IN	denotes	in
T10447	21920-21924	DT	denotes	each
T10448	21925-21935	NN	denotes	experiment
T10449	21935-21936	-RRB-	denotes	)
T10450	21936-21937	.	denotes	.
T4415	21950-21960	NN	denotes	CbCln3Δex7
T4417	21960-21961	HYPH	denotes	/
T4418	21961-21962	CD	denotes	8
T4419	21963-21973	JJ	denotes	cerebellar
T4420	21974-21983	NN	denotes	precursor
T4416	21984-21989	NNS	denotes	cells
T4421	21990-21999	VBP	denotes	represent
T4422	22000-22003	DT	denotes	the
T4424	22004-22009	JJ	denotes	first
T4425	22010-22021	RB	denotes	genetically
T4426	22022-22030	JJ	denotes	accurate
T4427	22031-22037	NN	denotes	neuron
T4429	22037-22038	HYPH	denotes	-
T4428	22038-22045	JJ	denotes	derived
T4430	22046-22053	NN	denotes	culture
T4423	22054-22059	NN	denotes	model
T4431	22060-22062	IN	denotes	of
T4432	22063-22067	NN	denotes	JNCL
T4433	22067-22068	.	denotes	.
T4434	22068-22220	sentence	denotes	Homozygous CbCln3Δex7/8 cells express mutant battenin and JNCL-hallmark mitochondrial ATPase subunit c accumulation, upon aging of cells at confluency.
T4435	22069-22079	JJ	denotes	Homozygous
T4437	22080-22090	NN	denotes	CbCln3Δex7
T4438	22090-22091	HYPH	denotes	/
T4439	22091-22092	CD	denotes	8
T4436	22093-22098	NNS	denotes	cells
T4440	22099-22106	VBP	denotes	express
T4441	22107-22113	NN	denotes	mutant
T4442	22114-22122	NN	denotes	battenin
T4443	22123-22126	CC	denotes	and
T4444	22127-22131	NN	denotes	JNCL
T4446	22131-22132	HYPH	denotes	-
T4445	22132-22140	NN	denotes	hallmark
T4448	22141-22154	JJ	denotes	mitochondrial
T4449	22155-22161	NN	denotes	ATPase
T4450	22162-22169	NN	denotes	subunit
T4451	22170-22171	NN	denotes	c
T4447	22172-22184	NN	denotes	accumulation
T4452	22184-22186	,	denotes	,
T4453	22186-22190	IN	denotes	upon
T4454	22191-22196	NN	denotes	aging
T4455	22197-22199	IN	denotes	of
T4456	22200-22205	NNS	denotes	cells
T4457	22206-22208	IN	denotes	at
T4458	22209-22219	NN	denotes	confluency
T4459	22219-22220	.	denotes	.
T4460	22220-22417	sentence	denotes	Moreover, this is the first study to indicate recessive endosomal/lysosomal membrane trafficking defects and mitochondrial dysfunction that precedes subunit c deposition in an accurate JNCL model.
T4461	22221-22229	RB	denotes	Moreover
T4463	22229-22231	,	denotes	,
T4464	22231-22235	DT	denotes	this
T4462	22236-22238	VBZ	denotes	is
T4465	22239-22242	DT	denotes	the
T4467	22243-22248	JJ	denotes	first
T4466	22249-22254	NN	denotes	study
T4468	22255-22257	TO	denotes	to
T4469	22258-22266	VB	denotes	indicate
T4470	22267-22276	JJ	denotes	recessive
T4472	22277-22286	JJ	denotes	endosomal
T4474	22286-22287	HYPH	denotes	/
T4473	22287-22296	JJ	denotes	lysosomal
T4475	22297-22305	NN	denotes	membrane
T4476	22306-22317	NN	denotes	trafficking
T4471	22318-22325	NNS	denotes	defects
T4477	22326-22329	CC	denotes	and
T4478	22330-22343	JJ	denotes	mitochondrial
T4479	22344-22355	NN	denotes	dysfunction
T4480	22356-22360	WDT	denotes	that
T4481	22361-22369	VBZ	denotes	precedes
T4482	22370-22377	NN	denotes	subunit
T4483	22378-22379	NN	denotes	c
T4484	22380-22390	NN	denotes	deposition
T4485	22391-22393	IN	denotes	in
T4486	22394-22396	DT	denotes	an
T4488	22397-22405	JJ	denotes	accurate
T4489	22406-22410	NN	denotes	JNCL
T4487	22411-22416	NN	denotes	model
T4490	22416-22417	.	denotes	.
T4491	22417-22549	sentence	denotes	Importantly, these defects are likely to be early events in the JNCL disease process and may particularly impact neuronal function.
T4492	22418-22429	RB	denotes	Importantly
T4494	22429-22431	,	denotes	,
T4495	22431-22436	DT	denotes	these
T4496	22437-22444	NNS	denotes	defects
T4493	22445-22448	VBP	denotes	are
T4497	22449-22455	JJ	denotes	likely
T4498	22456-22458	TO	denotes	to
T4499	22459-22461	VB	denotes	be
T4500	22462-22467	JJ	denotes	early
T4501	22468-22474	NNS	denotes	events
T4502	22475-22477	IN	denotes	in
T4503	22478-22481	DT	denotes	the
T4505	22482-22486	NN	denotes	JNCL
T4506	22487-22494	NN	denotes	disease
T4504	22495-22502	NN	denotes	process
T4507	22503-22506	CC	denotes	and
T4508	22507-22510	MD	denotes	may
T4510	22511-22523	RB	denotes	particularly
T4509	22524-22530	VB	denotes	impact
T4511	22531-22539	JJ	denotes	neuronal
T4512	22540-22548	NN	denotes	function
T4513	22548-22549	.	denotes	.
T4514	22549-22775	sentence	denotes	Abnormal cathepsin D localization and processing in homozygous CbCln3Δex7/8 cells and Cln3Δex7/8 mice likely reflects altered vesicular trafficking and/or lysosomal pH, which is known to impact cathepsin D processing [14,16].
T4515	22550-22558	JJ	denotes	Abnormal
T4517	22559-22568	NN	denotes	cathepsin
T4516	22569-22570	NN	denotes	D
T4519	22571-22583	NN	denotes	localization
T4520	22584-22587	CC	denotes	and
T4521	22588-22598	NN	denotes	processing
T4522	22599-22601	IN	denotes	in
T4523	22602-22612	JJ	denotes	homozygous
T4525	22613-22623	NN	denotes	CbCln3Δex7
T4526	22623-22624	HYPH	denotes	/
T4527	22624-22625	CD	denotes	8
T4524	22626-22631	NNS	denotes	cells
T4528	22632-22635	CC	denotes	and
T4529	22636-22644	NN	denotes	Cln3Δex7
T4531	22644-22645	HYPH	denotes	/
T4532	22645-22646	CD	denotes	8
T4530	22647-22651	NNS	denotes	mice
T4533	22652-22658	RB	denotes	likely
T4518	22659-22667	VBZ	denotes	reflects
T4534	22668-22675	VBN	denotes	altered
T4536	22676-22685	JJ	denotes	vesicular
T4535	22686-22697	NN	denotes	trafficking
T4537	22698-22701	CC	denotes	and
T4538	22701-22702	HYPH	denotes	/
T4539	22702-22704	CC	denotes	or
T4540	22705-22714	JJ	denotes	lysosomal
T4541	22715-22717	NN	denotes	pH
T4542	22717-22719	,	denotes	,
T4543	22719-22724	WDT	denotes	which
T4545	22725-22727	VBZ	denotes	is
T4544	22728-22733	VBN	denotes	known
T4546	22734-22736	TO	denotes	to
T4547	22737-22743	VB	denotes	impact
T4548	22744-22753	NN	denotes	cathepsin
T4549	22754-22755	NN	denotes	D
T4550	22756-22766	NN	denotes	processing
T4551	22767-22768	-LRB-	denotes	[
T4553	22768-22770	CD	denotes	14
T4554	22770-22771	,	denotes	,
T4552	22771-22773	CD	denotes	16
T4555	22773-22774	-RRB-	denotes	]
T4556	22774-22775	.	denotes	.
T4557	22775-22933	sentence	denotes	Indeed, CLN3 overexpression in HEK-293 cells altered lysosomal pH and cathepsin D processing [17], and lysosomal pH homeostasis is disrupted in JNCL [10,15].
T4558	22776-22782	RB	denotes	Indeed
T4560	22782-22784	,	denotes	,
T4561	22784-22788	NN	denotes	CLN3
T4562	22789-22803	NN	denotes	overexpression
T4563	22804-22806	IN	denotes	in
T4564	22807-22810	NN	denotes	HEK
T4566	22810-22811	HYPH	denotes	-
T4567	22811-22814	CD	denotes	293
T4565	22815-22820	NNS	denotes	cells
T4559	22821-22828	VBD	denotes	altered
T4568	22829-22838	JJ	denotes	lysosomal
T4569	22839-22841	NN	denotes	pH
T4571	22842-22845	CC	denotes	and
T4572	22846-22855	NN	denotes	cathepsin
T4573	22856-22857	NN	denotes	D
T4570	22858-22868	NN	denotes	processing
T4574	22869-22870	-LRB-	denotes	[
T4575	22870-22872	CD	denotes	17
T4576	22872-22873	-RRB-	denotes	]
T4577	22873-22875	,	denotes	,
T4578	22875-22878	CC	denotes	and
T4579	22879-22888	JJ	denotes	lysosomal
T4580	22889-22891	NN	denotes	pH
T4581	22892-22903	NN	denotes	homeostasis
T4583	22904-22906	VBZ	denotes	is
T4582	22907-22916	VBN	denotes	disrupted
T4584	22917-22919	IN	denotes	in
T4585	22920-22924	NN	denotes	JNCL
T4586	22925-22926	-LRB-	denotes	[
T4588	22926-22928	CD	denotes	10
T4589	22928-22929	,	denotes	,
T4587	22929-22931	CD	denotes	15
T4590	22931-22932	-RRB-	denotes	]
T4591	22932-22933	.	denotes	.
T4592	22933-23036	sentence	denotes	It is noteworthy that cathepsin B and the CLN2-encoded enzyme, TPPI, are also altered in JNCL [18-20].
T4593	22934-22936	PRP	denotes	It
T4594	22937-22939	VBZ	denotes	is
T4595	22940-22950	JJ	denotes	noteworthy
T4596	22951-22955	IN	denotes	that
T4598	22956-22965	NN	denotes	cathepsin
T4599	22966-22967	NN	denotes	B
T4600	22968-22971	CC	denotes	and
T4601	22972-22975	DT	denotes	the
T4603	22976-22980	NN	denotes	CLN2
T4605	22980-22981	HYPH	denotes	-
T4604	22981-22988	VBN	denotes	encoded
T4602	22989-22995	NN	denotes	enzyme
T4606	22995-22997	,	denotes	,
T4607	22997-23001	NN	denotes	TPPI
T4608	23001-23003	,	denotes	,
T4609	23003-23006	VBP	denotes	are
T4610	23007-23011	RB	denotes	also
T4597	23012-23019	VBN	denotes	altered
T4611	23020-23022	IN	denotes	in
T4612	23023-23027	NN	denotes	JNCL
T4613	23028-23029	-LRB-	denotes	[
T4614	23029-23031	CD	denotes	18
T4615	23031-23032	SYM	denotes	-
T4616	23032-23034	CD	denotes	20
T4617	23034-23035	-RRB-	denotes	]
T4618	23035-23036	.	denotes	.
T4619	23036-23208	sentence	denotes	Nevertheless, despite the cathepsin D protein alterations that are observed in homozygous CbCln3Δex7/8 cells, cathepsin D enzymatic activity does not appear to be reduced.
T4620	23037-23049	RB	denotes	Nevertheless
T4622	23049-23051	,	denotes	,
T4623	23051-23058	IN	denotes	despite
T4624	23059-23062	DT	denotes	the
T4626	23063-23072	NN	denotes	cathepsin
T4627	23073-23074	NN	denotes	D
T4628	23075-23082	NN	denotes	protein
T4625	23083-23094	NNS	denotes	alterations
T4629	23095-23099	WDT	denotes	that
T4631	23100-23103	VBP	denotes	are
T4630	23104-23112	VBN	denotes	observed
T4632	23113-23115	IN	denotes	in
T4633	23116-23126	JJ	denotes	homozygous
T4635	23127-23137	NN	denotes	CbCln3Δex7
T4636	23137-23138	HYPH	denotes	/
T4637	23138-23139	CD	denotes	8
T4634	23140-23145	NNS	denotes	cells
T4638	23145-23147	,	denotes	,
T4639	23147-23156	NN	denotes	cathepsin
T4640	23157-23158	NN	denotes	D
T4642	23159-23168	JJ	denotes	enzymatic
T4641	23169-23177	NN	denotes	activity
T4643	23178-23182	VBZ	denotes	does
T4644	23183-23186	RB	denotes	not
T4621	23187-23193	VB	denotes	appear
T4645	23194-23196	TO	denotes	to
T4647	23197-23199	VB	denotes	be
T4646	23200-23207	VBN	denotes	reduced
T4648	23207-23208	.	denotes	.
T4649	23208-23304	sentence	denotes	Thus, decreased cathepsin D activity is unlikely to account for subunit c accumulation in JNCL.
T4650	23209-23213	RB	denotes	Thus
T4652	23213-23215	,	denotes	,
T4653	23215-23224	VBN	denotes	decreased
T4655	23225-23234	NN	denotes	cathepsin
T4656	23235-23236	NN	denotes	D
T4654	23237-23245	NN	denotes	activity
T4651	23246-23248	VBZ	denotes	is
T4657	23249-23257	JJ	denotes	unlikely
T4658	23258-23260	TO	denotes	to
T4659	23261-23268	VB	denotes	account
T4660	23269-23272	IN	denotes	for
T4661	23273-23280	NN	denotes	subunit
T4662	23281-23282	NN	denotes	c
T4663	23283-23295	NN	denotes	accumulation
T4664	23296-23298	IN	denotes	in
T4665	23299-23303	NN	denotes	JNCL
T4666	23303-23304	.	denotes	.
T4667	23304-23425	sentence	denotes	Aging of homozygous CbCln3Δex7/8 cells at confluency is necessary to induce significantly accumulated subunit c protein.
T4668	23305-23310	NN	denotes	Aging
T4670	23311-23313	IN	denotes	of
T4671	23314-23324	JJ	denotes	homozygous
T4673	23325-23335	NN	denotes	CbCln3Δex7
T4674	23335-23336	HYPH	denotes	/
T4675	23336-23337	CD	denotes	8
T4672	23338-23343	NNS	denotes	cells
T4676	23344-23346	IN	denotes	at
T4677	23347-23357	NN	denotes	confluency
T4669	23358-23360	VBZ	denotes	is
T4678	23361-23370	JJ	denotes	necessary
T4679	23371-23373	TO	denotes	to
T4680	23374-23380	VB	denotes	induce
T4681	23381-23394	RB	denotes	significantly
T4682	23395-23406	VBN	denotes	accumulated
T4684	23407-23414	NN	denotes	subunit
T4685	23415-23416	NN	denotes	c
T4683	23417-23424	NN	denotes	protein
T4686	23424-23425	.	denotes	.
T4687	23425-23583	sentence	denotes	However, membrane organelle disruptions precede subunit c accumulation in homozygous CbCln3Δex7/8 cells, since they are observed without aging at confluency.
T4688	23426-23433	RB	denotes	However
T4690	23433-23435	,	denotes	,
T4691	23435-23443	NN	denotes	membrane
T4692	23444-23453	NN	denotes	organelle
T4693	23454-23465	NNS	denotes	disruptions
T4689	23466-23473	VBP	denotes	precede
T4694	23474-23481	NN	denotes	subunit
T4695	23482-23483	NN	denotes	c
T4696	23484-23496	NN	denotes	accumulation
T4697	23497-23499	IN	denotes	in
T4698	23500-23510	JJ	denotes	homozygous
T4700	23511-23521	NN	denotes	CbCln3Δex7
T4701	23521-23522	HYPH	denotes	/
T4702	23522-23523	CD	denotes	8
T4699	23524-23529	NNS	denotes	cells
T4703	23529-23531	,	denotes	,
T4704	23531-23536	IN	denotes	since
T4706	23537-23541	PRP	denotes	they
T4707	23542-23545	VBP	denotes	are
T4705	23546-23554	VBN	denotes	observed
T4708	23555-23562	IN	denotes	without
T4709	23563-23568	VBG	denotes	aging
T4710	23569-23571	IN	denotes	at
T4711	23572-23582	NN	denotes	confluency
T4712	23582-23583	.	denotes	.
T4713	23583-23764	sentence	denotes	Lysosomal and endosomal size and distribution are altered, and mitochondria are abnormally elongated and functionally compromised in sub-confluent homozygous CbCln3Δex7/8 cultures.
T4714	23584-23593	JJ	denotes	Lysosomal
T4716	23594-23597	CC	denotes	and
T4717	23598-23607	JJ	denotes	endosomal
T4715	23608-23612	NN	denotes	size
T4719	23613-23616	CC	denotes	and
T4720	23617-23629	NN	denotes	distribution
T4721	23630-23633	VBP	denotes	are
T4718	23634-23641	VBN	denotes	altered
T4722	23641-23643	,	denotes	,
T4723	23643-23646	CC	denotes	and
T4724	23647-23659	NNS	denotes	mitochondria
T4725	23660-23663	VBP	denotes	are
T4726	23664-23674	RB	denotes	abnormally
T4727	23675-23684	VBN	denotes	elongated
T4728	23685-23688	CC	denotes	and
T4729	23689-23701	RB	denotes	functionally
T4730	23702-23713	VBN	denotes	compromised
T4731	23714-23716	IN	denotes	in
T4732	23717-23730	JJ	denotes	sub-confluent
T4734	23731-23741	JJ	denotes	homozygous
T4735	23742-23752	NN	denotes	CbCln3Δex7
T4736	23752-23753	HYPH	denotes	/
T4737	23753-23754	CD	denotes	8
T4733	23755-23763	NNS	denotes	cultures
T4738	23763-23764	.	denotes	.
T4739	23764-23987	sentence	denotes	These observations argue that membrane trafficking defects do not result from excessive subunit c accumulation compromising the lysosome, but rather are early events in the disease process preceding subunit c accumulation.
T4740	23765-23770	DT	denotes	These
T4741	23771-23783	NNS	denotes	observations
T4742	23784-23789	VBP	denotes	argue
T4743	23790-23794	IN	denotes	that
T4745	23795-23803	NN	denotes	membrane
T4746	23804-23815	NN	denotes	trafficking
T4747	23816-23823	NNS	denotes	defects
T4748	23824-23826	VBP	denotes	do
T4749	23827-23830	RB	denotes	not
T4744	23831-23837	VB	denotes	result
T4750	23838-23842	IN	denotes	from
T4751	23843-23852	JJ	denotes	excessive
T4753	23853-23860	NN	denotes	subunit
T4754	23861-23862	NN	denotes	c
T4752	23863-23875	NN	denotes	accumulation
T4755	23876-23888	VBG	denotes	compromising
T4756	23889-23892	DT	denotes	the
T4757	23893-23901	NN	denotes	lysosome
T4758	23901-23903	,	denotes	,
T4759	23903-23906	CC	denotes	but
T4760	23907-23913	RB	denotes	rather
T4761	23914-23917	VBP	denotes	are
T4762	23918-23923	JJ	denotes	early
T4763	23924-23930	NNS	denotes	events
T4764	23931-23933	IN	denotes	in
T4765	23934-23937	DT	denotes	the
T4767	23938-23945	NN	denotes	disease
T4766	23946-23953	NN	denotes	process
T4768	23954-23963	VBG	denotes	preceding
T4769	23964-23971	NN	denotes	subunit
T4770	23972-23973	NN	denotes	c
T4771	23974-23986	NN	denotes	accumulation
T4772	23986-23987	.	denotes	.
T4773	23987-24182	sentence	denotes	Mitochondrial abnormalities, which have also been reported in JNCL patients and other animal models [21-23], may result from ineffective turnover by autophagy, a lysosomal-targeted pathway [24].
T4774	23988-24001	JJ	denotes	Mitochondrial
T4775	24002-24015	NNS	denotes	abnormalities
T4777	24015-24017	,	denotes	,
T4778	24017-24022	WDT	denotes	which
T4780	24023-24027	VBP	denotes	have
T4781	24028-24032	RB	denotes	also
T4782	24033-24037	VBN	denotes	been
T4779	24038-24046	VBN	denotes	reported
T4783	24047-24049	IN	denotes	in
T4784	24050-24054	NN	denotes	JNCL
T4785	24055-24063	NNS	denotes	patients
T4786	24064-24067	CC	denotes	and
T4787	24068-24073	JJ	denotes	other
T4789	24074-24080	NN	denotes	animal
T4788	24081-24087	NNS	denotes	models
T4790	24088-24089	-LRB-	denotes	[
T4791	24089-24091	CD	denotes	21
T4792	24091-24092	SYM	denotes	-
T4793	24092-24094	CD	denotes	23
T4794	24094-24095	-RRB-	denotes	]
T4795	24095-24097	,	denotes	,
T4796	24097-24100	MD	denotes	may
T4776	24101-24107	VB	denotes	result
T4797	24108-24112	IN	denotes	from
T4798	24113-24124	JJ	denotes	ineffective
T4799	24125-24133	NN	denotes	turnover
T4800	24134-24136	IN	denotes	by
T4801	24137-24146	NN	denotes	autophagy
T4802	24146-24148	,	denotes	,
T4803	24148-24149	DT	denotes	a
T4805	24150-24159	JJ	denotes	lysosomal
T4807	24159-24160	HYPH	denotes	-
T4806	24160-24168	VBN	denotes	targeted
T4804	24169-24176	NN	denotes	pathway
T4808	24177-24178	-LRB-	denotes	[
T4809	24178-24180	CD	denotes	24
T4810	24180-24181	-RRB-	denotes	]
T4811	24181-24182	.	denotes	.
T4812	24182-24394	sentence	denotes	Alternatively, or simultaneously, battenin deficiency may impact mitochondrial function upstream of turnover, affecting mitochondrial biogenesis and/or altered transport and processing of mitochondrial proteins.
T4813	24183-24196	RB	denotes	Alternatively
T4815	24196-24198	,	denotes	,
T4816	24198-24200	CC	denotes	or
T4817	24201-24215	RB	denotes	simultaneously
T4818	24215-24217	,	denotes	,
T4819	24217-24225	NN	denotes	battenin
T4820	24226-24236	NN	denotes	deficiency
T4821	24237-24240	MD	denotes	may
T4814	24241-24247	VB	denotes	impact
T4822	24248-24261	JJ	denotes	mitochondrial
T4823	24262-24270	NN	denotes	function
T4824	24271-24279	RB	denotes	upstream
T4825	24280-24282	IN	denotes	of
T4826	24283-24291	NN	denotes	turnover
T4827	24291-24293	,	denotes	,
T4828	24293-24302	VBG	denotes	affecting
T4829	24303-24316	JJ	denotes	mitochondrial
T4830	24317-24327	NN	denotes	biogenesis
T4831	24328-24331	CC	denotes	and
T4832	24331-24332	HYPH	denotes	/
T4833	24332-24334	CC	denotes	or
T4834	24335-24342	VBN	denotes	altered
T4835	24343-24352	NN	denotes	transport
T4836	24353-24356	CC	denotes	and
T4837	24357-24367	NN	denotes	processing
T4838	24368-24370	IN	denotes	of
T4839	24371-24384	JJ	denotes	mitochondrial
T4840	24385-24393	NN	denotes	proteins
T4841	24393-24394	.	denotes	.
T4842	24394-24502	sentence	denotes	In wild-type CbCln3 neuronal precursor cells battenin primarily co-localizes with early and late endosomes.
T4843	24395-24397	IN	denotes	In
T4845	24398-24402	JJ	denotes	wild
T4847	24402-24403	HYPH	denotes	-
T4846	24403-24407	NN	denotes	type
T4849	24408-24414	NN	denotes	CbCln3
T4850	24415-24423	JJ	denotes	neuronal
T4851	24424-24433	NN	denotes	precursor
T4848	24434-24439	NNS	denotes	cells
T4852	24440-24448	NN	denotes	battenin
T4853	24449-24458	RB	denotes	primarily
T4844	24459-24471	VBZ	denotes	co-localizes
T4854	24472-24476	IN	denotes	with
T4855	24477-24482	JJ	denotes	early
T4857	24483-24486	CC	denotes	and
T4858	24487-24491	JJ	denotes	late
T4856	24492-24501	NNS	denotes	endosomes
T4859	24501-24502	.	denotes	.
T4860	24502-24775	sentence	denotes	Battenin immunostaining in homozygous CbCln3Δex7/8 neuronal precursors is significantly reduced in abundance, but mutant signal also co-localizes with endosomal markers suggesting mutant battenin protein with C-terminal epitopes is trafficked similar to wild-type protein.
T4861	24503-24511	NN	denotes	Battenin
T4862	24512-24526	NN	denotes	immunostaining
T4864	24527-24529	IN	denotes	in
T4865	24530-24540	JJ	denotes	homozygous
T4867	24541-24551	NN	denotes	CbCln3Δex7
T4868	24551-24552	HYPH	denotes	/
T4869	24552-24553	CD	denotes	8
T4870	24554-24562	JJ	denotes	neuronal
T4866	24563-24573	NNS	denotes	precursors
T4871	24574-24576	VBZ	denotes	is
T4872	24577-24590	RB	denotes	significantly
T4863	24591-24598	VBN	denotes	reduced
T4873	24599-24601	IN	denotes	in
T4874	24602-24611	NN	denotes	abundance
T4875	24611-24613	,	denotes	,
T4876	24613-24616	CC	denotes	but
T4877	24617-24623	NN	denotes	mutant
T4878	24624-24630	NN	denotes	signal
T4880	24631-24635	RB	denotes	also
T4879	24636-24648	VBZ	denotes	co-localizes
T4881	24649-24653	IN	denotes	with
T4882	24654-24663	JJ	denotes	endosomal
T4883	24664-24671	NNS	denotes	markers
T4884	24672-24682	VBG	denotes	suggesting
T4885	24683-24689	NN	denotes	mutant
T4887	24690-24698	NN	denotes	battenin
T4886	24699-24706	NN	denotes	protein
T4889	24707-24711	IN	denotes	with
T4890	24712-24713	NN	denotes	C
T4892	24713-24714	HYPH	denotes	-
T4891	24714-24722	JJ	denotes	terminal
T4893	24723-24731	NNS	denotes	epitopes
T4894	24732-24734	VBZ	denotes	is
T4888	24735-24745	VBN	denotes	trafficked
T4895	24746-24753	JJ	denotes	similar
T4896	24754-24756	IN	denotes	to
T4897	24757-24761	JJ	denotes	wild
T4899	24761-24762	HYPH	denotes	-
T4898	24762-24766	NN	denotes	type
T4900	24767-24774	NN	denotes	protein
T4901	24774-24775	.	denotes	.
T4902	24775-24996	sentence	denotes	In other studies, CLN3/battenin protein localization has been reported to partially overlap with late endosomes and lysosomes in non-neuronal cells [7], and to lysosomes, synaptosomes [8] and endosomes [9,25] in neurons.
T4903	24776-24778	IN	denotes	In
T4905	24779-24784	JJ	denotes	other
T4906	24785-24792	NNS	denotes	studies
T4907	24792-24794	,	denotes	,
T4908	24794-24798	NN	denotes	CLN3
T4910	24798-24799	HYPH	denotes	/
T4911	24799-24807	NN	denotes	battenin
T4912	24808-24815	NN	denotes	protein
T4909	24816-24828	NN	denotes	localization
T4913	24829-24832	VBZ	denotes	has
T4914	24833-24837	VBN	denotes	been
T4904	24838-24846	VBN	denotes	reported
T4915	24847-24849	TO	denotes	to
T4917	24850-24859	RB	denotes	partially
T4916	24860-24867	VB	denotes	overlap
T4918	24868-24872	IN	denotes	with
T4919	24873-24877	JJ	denotes	late
T4920	24878-24887	NNS	denotes	endosomes
T4921	24888-24891	CC	denotes	and
T4922	24892-24901	NNS	denotes	lysosomes
T4923	24902-24904	IN	denotes	in
T4924	24905-24917	JJ	denotes	non-neuronal
T4925	24918-24923	NNS	denotes	cells
T4926	24924-24925	-LRB-	denotes	[
T4927	24925-24926	CD	denotes	7
T4928	24926-24927	-RRB-	denotes	]
T4929	24927-24929	,	denotes	,
T4930	24929-24932	CC	denotes	and
T4931	24933-24935	IN	denotes	to
T4932	24936-24945	NNS	denotes	lysosomes
T4933	24945-24947	,	denotes	,
T4934	24947-24959	NNS	denotes	synaptosomes
T4935	24960-24961	-LRB-	denotes	[
T4936	24961-24962	CD	denotes	8
T4937	24962-24963	-RRB-	denotes	]
T4938	24964-24967	CC	denotes	and
T4939	24968-24977	NNS	denotes	endosomes
T4940	24978-24979	-LRB-	denotes	[
T4942	24979-24980	CD	denotes	9
T4943	24980-24981	,	denotes	,
T4941	24981-24983	CD	denotes	25
T4944	24983-24984	-RRB-	denotes	]
T4945	24985-24987	IN	denotes	in
T4946	24988-24995	NNS	denotes	neurons
T4947	24995-24996	.	denotes	.
T4948	24996-25190	sentence	denotes	These data jointly indicate that battenin resides in a subset of vesicular compartments linking multiple membrane trafficking pathways, perhaps functioning in vesicular transport and/or fusion.
T4949	24997-25002	DT	denotes	These
T4950	25003-25007	NNS	denotes	data
T4952	25008-25015	RB	denotes	jointly
T4951	25016-25024	VBP	denotes	indicate
T4953	25025-25029	IN	denotes	that
T4955	25030-25038	NN	denotes	battenin
T4954	25039-25046	VBZ	denotes	resides
T4956	25047-25049	IN	denotes	in
T4957	25050-25051	DT	denotes	a
T4958	25052-25058	NN	denotes	subset
T4959	25059-25061	IN	denotes	of
T4960	25062-25071	JJ	denotes	vesicular
T4961	25072-25084	NNS	denotes	compartments
T4962	25085-25092	VBG	denotes	linking
T4963	25093-25101	JJ	denotes	multiple
T4965	25102-25110	NN	denotes	membrane
T4966	25111-25122	NN	denotes	trafficking
T4964	25123-25131	NNS	denotes	pathways
T4967	25131-25133	,	denotes	,
T4968	25133-25140	RB	denotes	perhaps
T4969	25141-25152	VBG	denotes	functioning
T4970	25153-25155	IN	denotes	in
T4971	25156-25165	JJ	denotes	vesicular
T4972	25166-25175	NN	denotes	transport
T4973	25176-25179	CC	denotes	and
T4974	25179-25180	HYPH	denotes	/
T4975	25180-25182	CC	denotes	or
T4976	25183-25189	NN	denotes	fusion
T4977	25189-25190	.	denotes	.
T4978	25190-25311	sentence	denotes	Endocytic and lysosomal-targeted pathways, including mitochondrial autophagy, are specifically implicated in this study.
T4979	25191-25200	JJ	denotes	Endocytic
T4981	25201-25204	CC	denotes	and
T4982	25205-25214	JJ	denotes	lysosomal
T4983	25214-25215	HYPH	denotes	-
T4980	25215-25223	VBN	denotes	targeted
T4984	25224-25232	NNS	denotes	pathways
T4986	25232-25234	,	denotes	,
T4987	25234-25243	VBG	denotes	including
T4988	25244-25257	JJ	denotes	mitochondrial
T4989	25258-25267	NN	denotes	autophagy
T4990	25267-25269	,	denotes	,
T4991	25269-25272	VBP	denotes	are
T4992	25273-25285	RB	denotes	specifically
T4985	25286-25296	VBN	denotes	implicated
T4993	25297-25299	IN	denotes	in
T4994	25300-25304	DT	denotes	this
T4995	25305-25310	NN	denotes	study
T4996	25310-25311	.	denotes	.
T5047	25325-25328	DT	denotes	The
T5049	25329-25337	NN	denotes	membrane
T5048	25338-25349	NN	denotes	trafficking
T5051	25350-25353	CC	denotes	and
T5052	25354-25367	JJ	denotes	mitochondrial
T5053	25368-25376	NNS	denotes	deficits
T5054	25377-25386	VBN	denotes	uncovered
T5055	25387-25389	IN	denotes	in
T5056	25390-25400	JJ	denotes	homozygous
T5058	25401-25411	NN	denotes	CbCln3Δex7
T5059	25411-25412	HYPH	denotes	/
T5060	25412-25413	CD	denotes	8
T5057	25414-25419	NNS	denotes	cells
T5050	25420-25423	VBP	denotes	are
T5061	25424-25430	JJ	denotes	likely
T5062	25431-25433	TO	denotes	to
T5064	25434-25446	RB	denotes	particularly
T5063	25447-25453	VB	denotes	impact
T5065	25454-25462	JJ	denotes	neuronal
T5066	25463-25471	NN	denotes	function
T5067	25471-25472	.	denotes	.
T5068	25472-25629	sentence	denotes	Neurotransmission heavily relies on membrane vesicle transport, and a high-energy metabolism may further sensitize neurons to the loss of battenin activity.
T5069	25473-25490	NN	denotes	Neurotransmission
T5071	25491-25498	RB	denotes	heavily
T5070	25499-25505	VBZ	denotes	relies
T5072	25506-25508	IN	denotes	on
T5073	25509-25517	NN	denotes	membrane
T5074	25518-25525	NN	denotes	vesicle
T5075	25526-25535	NN	denotes	transport
T5076	25535-25537	,	denotes	,
T5077	25537-25540	CC	denotes	and
T5078	25541-25542	DT	denotes	a
T5080	25543-25547	JJ	denotes	high
T5082	25547-25548	HYPH	denotes	-
T5081	25548-25554	NN	denotes	energy
T5079	25555-25565	NN	denotes	metabolism
T5084	25566-25569	MD	denotes	may
T5085	25570-25577	RB	denotes	further
T5083	25578-25587	VB	denotes	sensitize
T5086	25588-25595	NNS	denotes	neurons
T5087	25596-25598	IN	denotes	to
T5088	25599-25602	DT	denotes	the
T5089	25603-25607	NN	denotes	loss
T5090	25608-25610	IN	denotes	of
T5091	25611-25619	NN	denotes	battenin
T5092	25620-25628	NN	denotes	activity
T5093	25628-25629	.	denotes	.
T5094	25629-25810	sentence	denotes	Thus, our panel of wild-type, heterozygous, and homozygous CbCln3Δex7/8 cerebellar cells provide an ideal model system to further elucidate battenin function and JNCL pathogenesis.
T5095	25630-25634	RB	denotes	Thus
T5097	25634-25636	,	denotes	,
T5098	25636-25639	PRP$	denotes	our
T5099	25640-25645	NN	denotes	panel
T5100	25646-25648	IN	denotes	of
T5101	25649-25653	JJ	denotes	wild
T5103	25653-25654	HYPH	denotes	-
T5102	25654-25658	NN	denotes	type
T5105	25658-25660	,	denotes	,
T5106	25660-25672	JJ	denotes	heterozygous
T5107	25672-25674	,	denotes	,
T5108	25674-25677	CC	denotes	and
T5109	25678-25688	JJ	denotes	homozygous
T5110	25689-25699	NN	denotes	CbCln3Δex7
T5111	25699-25700	HYPH	denotes	/
T5112	25700-25701	CD	denotes	8
T5113	25702-25712	JJ	denotes	cerebellar
T5104	25713-25718	NNS	denotes	cells
T5096	25719-25726	VBP	denotes	provide
T5114	25727-25729	DT	denotes	an
T5116	25730-25735	JJ	denotes	ideal
T5117	25736-25741	NN	denotes	model
T5115	25742-25748	NN	denotes	system
T5118	25749-25751	TO	denotes	to
T5120	25752-25759	RBR	denotes	further
T5119	25760-25769	VB	denotes	elucidate
T5121	25770-25778	NN	denotes	battenin
T5122	25779-25787	NN	denotes	function
T5123	25788-25791	CC	denotes	and
T5124	25792-25796	NN	denotes	JNCL
T5125	25797-25809	NN	denotes	pathogenesis
T5126	25809-25810	.	denotes	.
T5236	25821-25829	NN	denotes	Antibody
T5238	25830-25833	CC	denotes	and
T5239	25834-25838	NN	denotes	cell
T5240	25839-25847	NN	denotes	staining
T5237	25848-25856	NNS	denotes	reagents
T5241	25856-26110	sentence	denotes	Nestin (clone Rat 401, 2 μg/ml), Lamp 1 (clone 1D4B, 6 μg/ml), and Lamp 2 (clone Abl-93; 6 μg/ml) monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, maintained by The University of Iowa, Department of Biological Sciences.
T5242	25857-25863	NN	denotes	Nestin
T5244	25864-25865	-LRB-	denotes	(
T5246	25865-25870	NN	denotes	clone
T5247	25871-25874	NN	denotes	Rat
T5248	25875-25878	CD	denotes	401
T5249	25878-25880	,	denotes	,
T5250	25880-25881	CD	denotes	2
T5245	25882-25884	NN	denotes	μg
T5251	25884-25885	SYM	denotes	/
T5252	25885-25887	NN	denotes	ml
T5253	25887-25888	-RRB-	denotes	)
T5254	25888-25890	,	denotes	,
T5255	25890-25894	NN	denotes	Lamp
T5256	25895-25896	CD	denotes	1
T5257	25897-25898	-LRB-	denotes	(
T5259	25898-25903	NN	denotes	clone
T5260	25904-25908	NN	denotes	1D4B
T5261	25908-25910	,	denotes	,
T5262	25910-25911	CD	denotes	6
T5258	25912-25914	NN	denotes	μg
T5263	25914-25915	SYM	denotes	/
T5264	25915-25917	NN	denotes	ml
T5265	25917-25918	-RRB-	denotes	)
T5266	25918-25920	,	denotes	,
T5267	25920-25923	CC	denotes	and
T5268	25924-25928	NN	denotes	Lamp
T5269	25929-25930	CD	denotes	2
T5270	25931-25932	-LRB-	denotes	(
T5272	25932-25937	NN	denotes	clone
T5273	25938-25941	NN	denotes	Abl
T5274	25941-25942	HYPH	denotes	-
T5275	25942-25944	CD	denotes	93
T5276	25944-25945	:	denotes	;
T5277	25946-25947	CD	denotes	6
T5271	25948-25950	NN	denotes	μg
T5278	25950-25951	SYM	denotes	/
T5279	25951-25953	NN	denotes	ml
T5280	25953-25954	-RRB-	denotes	)
T5281	25955-25965	JJ	denotes	monoclonal
T5243	25966-25976	NNS	denotes	antibodies
T5283	25977-25981	VBD	denotes	were
T5282	25982-25990	VBN	denotes	obtained
T5284	25991-25995	IN	denotes	from
T5285	25996-25999	DT	denotes	the
T5287	26000-26013	NNP	denotes	Developmental
T5288	26014-26021	NNPS	denotes	Studies
T5289	26022-26031	NNP	denotes	Hybridoma
T5286	26032-26036	NNP	denotes	Bank
T5290	26036-26038	,	denotes	,
T5291	26038-26048	VBN	denotes	maintained
T5292	26049-26051	IN	denotes	by
T5293	26052-26055	DT	denotes	The
T5294	26056-26066	NNP	denotes	University
T5296	26067-26069	IN	denotes	of
T5297	26070-26074	NNP	denotes	Iowa
T5298	26074-26076	,	denotes	,
T5295	26076-26086	NNP	denotes	Department
T5299	26087-26089	IN	denotes	of
T5300	26090-26100	NNP	denotes	Biological
T5301	26101-26109	NNPS	denotes	Sciences
T5302	26109-26110	.	denotes	.
T5303	26110-26157	sentence	denotes	Batp1 (1 μg/ml) was previously described [12].
T5304	26111-26116	NN	denotes	Batp1
T5306	26117-26118	-LRB-	denotes	(
T5308	26118-26119	CD	denotes	1
T5307	26120-26122	NN	denotes	μg
T5309	26122-26123	SYM	denotes	/
T5310	26123-26125	NN	denotes	ml
T5311	26125-26126	-RRB-	denotes	)
T5312	26127-26130	VBD	denotes	was
T5313	26131-26141	RB	denotes	previously
T5305	26142-26151	VBN	denotes	described
T5314	26152-26153	-LRB-	denotes	[
T5315	26153-26155	CD	denotes	12
T5316	26155-26156	-RRB-	denotes	]
T5317	26156-26157	.	denotes	.
T5318	26157-26266	sentence	denotes	Anti-subunit c antibody (0.7 μg/ml) was kindly provided by Dr. Kominami (Juntendo University, Tokyo, Japan).
T5319	26158-26170	JJ	denotes	Anti-subunit
T5320	26171-26172	NN	denotes	c
T5321	26173-26181	NN	denotes	antibody
T5323	26182-26183	-LRB-	denotes	(
T5325	26183-26186	CD	denotes	0.7
T5324	26187-26189	NN	denotes	μg
T5326	26189-26190	SYM	denotes	/
T5327	26190-26192	NN	denotes	ml
T5328	26192-26193	-RRB-	denotes	)
T5329	26194-26197	VBD	denotes	was
T5330	26198-26204	RB	denotes	kindly
T5322	26205-26213	VBN	denotes	provided
T5331	26214-26216	IN	denotes	by
T5332	26217-26220	NNP	denotes	Dr.
T5333	26221-26229	NNP	denotes	Kominami
T5334	26230-26231	-LRB-	denotes	(
T5336	26231-26239	NNP	denotes	Juntendo
T5335	26240-26250	NNP	denotes	University
T5337	26250-26252	,	denotes	,
T5338	26252-26257	NNP	denotes	Tokyo
T5339	26257-26259	,	denotes	,
T5340	26259-26264	NNP	denotes	Japan
T5341	26264-26265	-RRB-	denotes	)
T5342	26265-26266	.	denotes	.
T5343	26266-26839	sentence	denotes	Additional antibodies were as follows: GFAP, 1:2000 (DAKO Corporation); calbindin, 1:5000 (Sigma); NeuN, 10 μg/ml (Chemicon); SV40 T antigen (Pab 101), 2 μg/ml (Santa Cruz Biotechnology); cathepsin D (C-20), 2 μg/ml (Santa Cruz Biotechnology); cytochrome c oxidase subunit IV (cox4), 1:1000 (BD Biosciences Clontech); PDI (H-160), 2 μg/ml (Santa Cruz Biotechnology); GM130, 1 μg/ml (BD Transduction Labs); α-tubulin, 1:15,000 (Sigma); grp75, 1:200 (Stressgen); early endosome antigen-1 (EEA1), 2 μg/ml (Santa Cruz Biotechnology); rab 7, 4 μg/ml (Santa Cruz Biotechnology).
T5344	26267-26277	JJ	denotes	Additional
T5345	26278-26288	NNS	denotes	antibodies
T5346	26289-26293	VBD	denotes	were
T5347	26294-26296	IN	denotes	as
T5348	26297-26304	VBZ	denotes	follows
T5349	26304-26306	:	denotes	:
T5350	26306-26310	NN	denotes	GFAP
T5351	26310-26312	,	denotes	,
T5352	26312-26313	CD	denotes	1
T5353	26313-26314	SYM	denotes	:
T5354	26314-26318	CD	denotes	2000
T5355	26319-26320	-LRB-	denotes	(
T5357	26320-26324	NN	denotes	DAKO
T5356	26325-26336	NN	denotes	Corporation
T5358	26336-26337	-RRB-	denotes	)
T5359	26337-26338	:	denotes	;
T5360	26339-26348	NN	denotes	calbindin
T5361	26348-26350	,	denotes	,
T5362	26350-26351	CD	denotes	1
T5363	26351-26352	SYM	denotes	:
T5364	26352-26356	CD	denotes	5000
T5365	26357-26358	-LRB-	denotes	(
T5366	26358-26363	NNP	denotes	Sigma
T5367	26363-26364	-RRB-	denotes	)
T5368	26364-26365	:	denotes	;
T5369	26366-26370	NN	denotes	NeuN
T5370	26370-26372	,	denotes	,
T5371	26372-26374	CD	denotes	10
T5372	26375-26377	NN	denotes	μg
T5373	26377-26378	SYM	denotes	/
T5374	26378-26380	NN	denotes	ml
T5375	26381-26382	-LRB-	denotes	(
T5376	26382-26390	NNP	denotes	Chemicon
T5377	26390-26391	-RRB-	denotes	)
T5378	26391-26392	:	denotes	;
T5379	26393-26397	NN	denotes	SV40
T5381	26398-26399	NN	denotes	T
T5380	26400-26407	NN	denotes	antigen
T5382	26408-26409	-LRB-	denotes	(
T5383	26409-26412	NN	denotes	Pab
T5384	26413-26416	CD	denotes	101
T5385	26416-26417	-RRB-	denotes	)
T5386	26417-26419	,	denotes	,
T5387	26419-26420	CD	denotes	2
T5388	26421-26423	NN	denotes	μg
T5389	26423-26424	SYM	denotes	/
T5390	26424-26426	NN	denotes	ml
T5391	26427-26428	-LRB-	denotes	(
T5393	26428-26433	NNP	denotes	Santa
T5394	26434-26438	NNP	denotes	Cruz
T5392	26439-26452	NNP	denotes	Biotechnology
T5395	26452-26453	-RRB-	denotes	)
T5396	26453-26454	:	denotes	;
T5397	26455-26464	NN	denotes	cathepsin
T5398	26465-26466	NN	denotes	D
T5399	26467-26468	-LRB-	denotes	(
T5400	26468-26469	NN	denotes	C
T5401	26469-26470	HYPH	denotes	-
T5402	26470-26472	CD	denotes	20
T5403	26472-26473	-RRB-	denotes	)
T5404	26473-26475	,	denotes	,
T5405	26475-26476	CD	denotes	2
T5406	26477-26479	NN	denotes	μg
T5407	26479-26480	SYM	denotes	/
T5408	26480-26482	NN	denotes	ml
T5409	26483-26484	-LRB-	denotes	(
T5411	26484-26489	NNP	denotes	Santa
T5412	26490-26494	NNP	denotes	Cruz
T5410	26495-26508	NNP	denotes	Biotechnology
T5413	26508-26509	-RRB-	denotes	)
T5414	26509-26510	:	denotes	;
T5415	26511-26521	NN	denotes	cytochrome
T5417	26522-26523	NN	denotes	c
T5418	26524-26531	NN	denotes	oxidase
T5416	26532-26539	NN	denotes	subunit
T5419	26540-26542	CD	denotes	IV
T5420	26543-26544	-LRB-	denotes	(
T5421	26544-26548	NN	denotes	cox4
T5422	26548-26549	-RRB-	denotes	)
T5423	26549-26551	,	denotes	,
T5424	26551-26552	CD	denotes	1
T5425	26552-26553	SYM	denotes	:
T5426	26553-26557	CD	denotes	1000
T5427	26558-26559	-LRB-	denotes	(
T5429	26559-26561	NN	denotes	BD
T5430	26562-26573	NNP	denotes	Biosciences
T5428	26574-26582	NNP	denotes	Clontech
T5431	26582-26583	-RRB-	denotes	)
T5432	26583-26584	:	denotes	;
T5433	26585-26588	NN	denotes	PDI
T5434	26589-26590	-LRB-	denotes	(
T5435	26590-26591	NN	denotes	H
T5436	26591-26592	HYPH	denotes	-
T5437	26592-26595	CD	denotes	160
T5438	26595-26596	-RRB-	denotes	)
T5439	26596-26598	,	denotes	,
T5440	26598-26599	CD	denotes	2
T5441	26600-26602	NN	denotes	μg
T5442	26602-26603	SYM	denotes	/
T5443	26603-26605	NN	denotes	ml
T5444	26606-26607	-LRB-	denotes	(
T5446	26607-26612	NNP	denotes	Santa
T5447	26613-26617	NNP	denotes	Cruz
T5445	26618-26631	NNP	denotes	Biotechnology
T5448	26631-26632	-RRB-	denotes	)
T5449	26632-26633	:	denotes	;
T5450	26634-26639	NN	denotes	GM130
T5451	26639-26641	,	denotes	,
T5452	26641-26642	CD	denotes	1
T5453	26643-26645	NN	denotes	μg
T5454	26645-26646	SYM	denotes	/
T5455	26646-26648	NN	denotes	ml
T5456	26649-26650	-LRB-	denotes	(
T5458	26650-26652	NN	denotes	BD
T5459	26653-26665	NN	denotes	Transduction
T5457	26666-26670	NNS	denotes	Labs
T5460	26670-26671	-RRB-	denotes	)
T5461	26671-26672	:	denotes	;
T5462	26673-26674	NN	denotes	α
T5464	26674-26675	HYPH	denotes	-
T5463	26675-26682	NN	denotes	tubulin
T5465	26682-26684	,	denotes	,
T5466	26684-26685	CD	denotes	1
T5467	26685-26686	SYM	denotes	:
T5468	26686-26692	CD	denotes	15,000
T5469	26693-26694	-LRB-	denotes	(
T5470	26694-26699	NNP	denotes	Sigma
T5471	26699-26700	-RRB-	denotes	)
T5472	26700-26701	:	denotes	;
T5473	26702-26707	NN	denotes	grp75
T5474	26707-26709	,	denotes	,
T5475	26709-26710	CD	denotes	1
T5476	26710-26711	SYM	denotes	:
T5477	26711-26714	CD	denotes	200
T5478	26715-26716	-LRB-	denotes	(
T5479	26716-26725	NNP	denotes	Stressgen
T5480	26725-26726	-RRB-	denotes	)
T5481	26726-26727	:	denotes	;
T5482	26728-26733	JJ	denotes	early
T5484	26734-26742	NN	denotes	endosome
T5483	26743-26750	NN	denotes	antigen
T5485	26750-26751	HYPH	denotes	-
T5486	26751-26752	CD	denotes	1
T5487	26753-26754	-LRB-	denotes	(
T5488	26754-26758	NN	denotes	EEA1
T5489	26758-26759	-RRB-	denotes	)
T5490	26759-26761	,	denotes	,
T5491	26761-26762	CD	denotes	2
T5492	26763-26765	NN	denotes	μg
T5493	26765-26766	SYM	denotes	/
T5494	26766-26768	NN	denotes	ml
T5495	26769-26770	-LRB-	denotes	(
T5497	26770-26775	NNP	denotes	Santa
T5498	26776-26780	NNP	denotes	Cruz
T5496	26781-26794	NNP	denotes	Biotechnology
T5499	26794-26795	-RRB-	denotes	)
T5500	26795-26796	:	denotes	;
T5501	26797-26800	NN	denotes	rab
T5502	26801-26802	CD	denotes	7
T5503	26802-26804	,	denotes	,
T5504	26804-26805	CD	denotes	4
T5505	26806-26808	NN	denotes	μg
T5506	26808-26809	SYM	denotes	/
T5507	26809-26811	NN	denotes	ml
T5508	26812-26813	-LRB-	denotes	(
T5510	26813-26818	NNP	denotes	Santa
T5511	26819-26823	NNP	denotes	Cruz
T5509	26824-26837	NNP	denotes	Biotechnology
T5512	26837-26838	-RRB-	denotes	)
T5513	26838-26839	.	denotes	.
T5514	26839-27012	sentence	denotes	All fluorescent secondary antibodies were obtained from Jackson ImmunoResearch Laboratories and HRP-conjugated secondary antibodies were obtained from Amersham Biosciences.
T5515	26840-26843	DT	denotes	All
T5517	26844-26855	JJ	denotes	fluorescent
T5518	26856-26865	JJ	denotes	secondary
T5516	26866-26876	NNS	denotes	antibodies
T5520	26877-26881	VBD	denotes	were
T5519	26882-26890	VBN	denotes	obtained
T5521	26891-26895	IN	denotes	from
T5522	26896-26903	NNP	denotes	Jackson
T5524	26904-26918	NNP	denotes	ImmunoResearch
T5523	26919-26931	NNP	denotes	Laboratories
T5525	26932-26935	CC	denotes	and
T5526	26936-26939	NN	denotes	HRP
T5528	26939-26940	HYPH	denotes	-
T5527	26940-26950	VBN	denotes	conjugated
T5530	26951-26960	JJ	denotes	secondary
T5529	26961-26971	NNS	denotes	antibodies
T5532	26972-26976	VBD	denotes	were
T5531	26977-26985	VBN	denotes	obtained
T5533	26986-26990	IN	denotes	from
T5534	26991-26999	NNP	denotes	Amersham
T5535	27000-27011	NNP	denotes	Biosciences
T5536	27011-27012	.	denotes	.
T5537	27012-27182	sentence	denotes	Additional cell markers were as follows: VVL-biotin, 1:2000 (Vector Laboratories), 10,000 MW dextran-FITC, 1 mg/ml and Lysotracker Red DND-99, 500 nM (Molecular Probes).
T5538	27013-27023	JJ	denotes	Additional
T5540	27024-27028	NN	denotes	cell
T5539	27029-27036	NNS	denotes	markers
T5541	27037-27041	VBD	denotes	were
T5542	27042-27044	IN	denotes	as
T5543	27045-27052	VBZ	denotes	follows
T5544	27052-27054	:	denotes	:
T5545	27054-27057	NN	denotes	VVL
T5547	27057-27058	HYPH	denotes	-
T5546	27058-27064	NN	denotes	biotin
T5548	27064-27066	,	denotes	,
T5549	27066-27067	CD	denotes	1
T5550	27067-27068	SYM	denotes	:
T5551	27068-27072	CD	denotes	2000
T5552	27073-27074	-LRB-	denotes	(
T5554	27074-27080	NNP	denotes	Vector
T5553	27081-27093	NNP	denotes	Laboratories
T5555	27093-27094	-RRB-	denotes	)
T5556	27094-27096	,	denotes	,
T5557	27096-27102	CD	denotes	10,000
T5558	27103-27105	NN	denotes	MW
T5560	27106-27113	NN	denotes	dextran
T5561	27113-27114	HYPH	denotes	-
T5559	27114-27118	NN	denotes	FITC
T5562	27118-27120	,	denotes	,
T5563	27120-27121	CD	denotes	1
T5564	27122-27124	NN	denotes	mg
T5565	27124-27125	SYM	denotes	/
T5566	27125-27127	NN	denotes	ml
T5567	27128-27131	CC	denotes	and
T5568	27132-27143	NNP	denotes	Lysotracker
T5570	27144-27147	NN	denotes	Red
T5569	27148-27151	NN	denotes	DND
T5571	27151-27152	HYPH	denotes	-
T5572	27152-27154	CD	denotes	99
T5573	27154-27156	,	denotes	,
T5574	27156-27159	CD	denotes	500
T5575	27160-27162	NN	denotes	nM
T5576	27163-27164	-LRB-	denotes	(
T5578	27164-27173	NNP	denotes	Molecular
T5577	27174-27180	NNP	denotes	Probes
T5579	27180-27181	-RRB-	denotes	)
T5580	27181-27182	.	denotes	.
T5713	27184-27194	NN	denotes	Generation
T5714	27194-27196	,	denotes	,
T5715	27196-27207	NN	denotes	maintenance
T5716	27208-27211	CC	denotes	and
T5717	27212-27227	NN	denotes	differentiation
T5718	27228-27230	IN	denotes	of
T5719	27231-27237	NN	denotes	CbCln3
T5721	27238-27248	JJ	denotes	cerebellar
T5722	27249-27257	JJ	denotes	neuronal
T5723	27258-27267	NN	denotes	precursor
T5720	27268-27273	NNS	denotes	lines
T5724	27273-27335	sentence	denotes	Cln3Δex7/8 knock-in mice have been previously described [12].
T5725	27274-27282	NN	denotes	Cln3Δex7
T5727	27282-27283	HYPH	denotes	/
T5728	27283-27284	CD	denotes	8
T5729	27285-27290	VB	denotes	knock
T5730	27290-27291	HYPH	denotes	-
T5731	27291-27293	RP	denotes	in
T5726	27294-27298	NNS	denotes	mice
T5733	27299-27303	VBP	denotes	have
T5734	27304-27308	VBN	denotes	been
T5735	27309-27319	RB	denotes	previously
T5732	27320-27329	VBN	denotes	described
T5736	27330-27331	-LRB-	denotes	[
T5737	27331-27333	CD	denotes	12
T5738	27333-27334	-RRB-	denotes	]
T5739	27334-27335	.	denotes	.
T5740	27335-27515	sentence	denotes	Littermate pups from heterozygote crosses were used for primary culture establishment, by previously described methods that yield cerebellar granule neuron-enriched cultures [26].
T5741	27336-27346	NN	denotes	Littermate
T5742	27347-27351	NNS	denotes	pups
T5744	27352-27356	IN	denotes	from
T5745	27357-27369	NN	denotes	heterozygote
T5746	27370-27377	NNS	denotes	crosses
T5747	27378-27382	VBD	denotes	were
T5743	27383-27387	VBN	denotes	used
T5748	27388-27391	IN	denotes	for
T5749	27392-27399	JJ	denotes	primary
T5751	27400-27407	NN	denotes	culture
T5750	27408-27421	NN	denotes	establishment
T5752	27421-27423	,	denotes	,
T5753	27423-27425	IN	denotes	by
T5754	27426-27436	RB	denotes	previously
T5755	27437-27446	VBN	denotes	described
T5756	27447-27454	NNS	denotes	methods
T5757	27455-27459	WDT	denotes	that
T5758	27460-27465	VBP	denotes	yield
T5759	27466-27476	JJ	denotes	cerebellar
T5761	27477-27484	NN	denotes	granule
T5760	27485-27491	NN	denotes	neuron
T5763	27491-27492	HYPH	denotes	-
T5762	27492-27500	VBN	denotes	enriched
T5764	27501-27509	NNS	denotes	cultures
T5765	27510-27511	-LRB-	denotes	[
T5766	27511-27513	CD	denotes	26
T5767	27513-27514	-RRB-	denotes	]
T5768	27514-27515	.	denotes	.
T5769	27515-27642	sentence	denotes	Postnatal day 4 (P4) cerebella were dissected in Hank's Balanced Salt Solution (HBSS, Sigma), supplemented with 35 mM glucose.
T5770	27516-27525	JJ	denotes	Postnatal
T5771	27526-27529	NN	denotes	day
T5773	27530-27531	CD	denotes	4
T5774	27532-27533	-LRB-	denotes	(
T5775	27533-27535	NN	denotes	P4
T5776	27535-27536	-RRB-	denotes	)
T5772	27537-27546	NNS	denotes	cerebella
T5778	27547-27551	VBD	denotes	were
T5777	27552-27561	VBN	denotes	dissected
T5779	27562-27564	IN	denotes	in
T5780	27565-27569	NNP	denotes	Hank
T5782	27569-27571	POS	denotes	's
T5783	27572-27580	NNP	denotes	Balanced
T5784	27581-27585	NNP	denotes	Salt
T5781	27586-27594	NNP	denotes	Solution
T5785	27595-27596	-LRB-	denotes	(
T5786	27596-27600	NN	denotes	HBSS
T5787	27600-27602	,	denotes	,
T5788	27602-27607	NNP	denotes	Sigma
T5789	27607-27608	-RRB-	denotes	)
T5790	27608-27610	,	denotes	,
T5791	27610-27622	VBN	denotes	supplemented
T5792	27623-27627	IN	denotes	with
T5793	27628-27630	CD	denotes	35
T5794	27631-27633	NN	denotes	mM
T5795	27634-27641	NN	denotes	glucose
T5796	27641-27642	.	denotes	.
T5797	27642-27726	sentence	denotes	Tail biopsies were also collected for genomic DNA isolation and genotypic analysis.
T5798	27643-27647	NN	denotes	Tail
T5799	27648-27656	NNS	denotes	biopsies
T5801	27657-27661	VBD	denotes	were
T5802	27662-27666	RB	denotes	also
T5800	27667-27676	VBN	denotes	collected
T5803	27677-27680	IN	denotes	for
T5804	27681-27688	JJ	denotes	genomic
T5806	27689-27692	NN	denotes	DNA
T5805	27693-27702	NN	denotes	isolation
T5807	27703-27706	CC	denotes	and
T5808	27707-27716	JJ	denotes	genotypic
T5809	27717-27725	NN	denotes	analysis
T5810	27725-27726	.	denotes	.
T5811	27726-27920	sentence	denotes	Trypsin/EDTA (10 mg/ml, Sigma) and DNase I (100 μg/ml, Sigma), suspended in HBSS, helped dissociate cerebella for primary culture plating onto 0.01% poly-ornithine (Sigma) coated 100 mm dishes.
T5812	27727-27734	NN	denotes	Trypsin
T5814	27734-27735	HYPH	denotes	/
T5813	27735-27739	NN	denotes	EDTA
T5816	27740-27741	-LRB-	denotes	(
T5818	27741-27743	CD	denotes	10
T5819	27744-27746	NN	denotes	mg
T5820	27746-27747	SYM	denotes	/
T5821	27747-27749	NN	denotes	ml
T5822	27749-27751	,	denotes	,
T5817	27751-27756	NNP	denotes	Sigma
T5823	27756-27757	-RRB-	denotes	)
T5824	27758-27761	CC	denotes	and
T5825	27762-27767	NN	denotes	DNase
T5826	27768-27769	CD	denotes	I
T5827	27770-27771	-LRB-	denotes	(
T5829	27771-27774	CD	denotes	100
T5830	27775-27777	NN	denotes	μg
T5831	27777-27778	SYM	denotes	/
T5832	27778-27780	NN	denotes	ml
T5833	27780-27782	,	denotes	,
T5828	27782-27787	NNP	denotes	Sigma
T5834	27787-27788	-RRB-	denotes	)
T5835	27788-27790	,	denotes	,
T5836	27790-27799	VBN	denotes	suspended
T5837	27800-27802	IN	denotes	in
T5838	27803-27807	NN	denotes	HBSS
T5839	27807-27809	,	denotes	,
T5815	27809-27815	VBD	denotes	helped
T5840	27816-27826	VB	denotes	dissociate
T5841	27827-27836	NNS	denotes	cerebella
T5842	27837-27840	IN	denotes	for
T5843	27841-27848	JJ	denotes	primary
T5844	27849-27856	NN	denotes	culture
T5845	27857-27864	NN	denotes	plating
T5846	27865-27869	IN	denotes	onto
T5847	27870-27874	CD	denotes	0.01
T5848	27874-27875	NN	denotes	%
T5849	27876-27890	NN	denotes	poly-ornithine
T5851	27891-27892	-LRB-	denotes	(
T5852	27892-27897	NNP	denotes	Sigma
T5853	27897-27898	-RRB-	denotes	)
T5850	27899-27905	VBN	denotes	coated
T5855	27906-27909	CD	denotes	100
T5856	27910-27912	NN	denotes	mm
T5854	27913-27919	NNS	denotes	dishes
T5857	27919-27920	.	denotes	.
T5858	27920-28169	sentence	denotes	Primary cultures from individual cerebella were cultured overnight at 37°C, 5% CO2, in granule neuron growth media (Dulbecco's Modified Eagle Medium [DMEM, Gibco BRL #11995-065], 10% fetal bovine serum [Sigma #F-2442], supplemented with 24 mM KCl).
T5859	27921-27928	JJ	denotes	Primary
T5860	27929-27937	NNS	denotes	cultures
T5862	27938-27942	IN	denotes	from
T5863	27943-27953	JJ	denotes	individual
T5864	27954-27963	NNS	denotes	cerebella
T5865	27964-27968	VBD	denotes	were
T5861	27969-27977	VBN	denotes	cultured
T5866	27978-27987	RB	denotes	overnight
T5867	27988-27990	IN	denotes	at
T5868	27991-27993	CD	denotes	37
T5869	27993-27995	NN	denotes	°C
T5870	27995-27997	,	denotes	,
T5871	27997-27998	CD	denotes	5
T5872	27998-27999	NN	denotes	%
T5873	28000-28003	NN	denotes	CO2
T5874	28003-28005	,	denotes	,
T5875	28005-28007	IN	denotes	in
T5876	28008-28015	NN	denotes	granule
T5877	28016-28022	NN	denotes	neuron
T5879	28023-28029	NN	denotes	growth
T5878	28030-28035	NNS	denotes	media
T5880	28036-28037	-LRB-	denotes	(
T5881	28037-28045	NNP	denotes	Dulbecco
T5883	28045-28047	POS	denotes	's
T5884	28048-28056	NNP	denotes	Modified
T5885	28057-28062	NNP	denotes	Eagle
T5882	28063-28069	NNP	denotes	Medium
T5886	28070-28071	-LRB-	denotes	[
T5887	28071-28075	NN	denotes	DMEM
T5888	28075-28077	,	denotes	,
T5889	28077-28082	NNP	denotes	Gibco
T5891	28083-28086	NNP	denotes	BRL
T5890	28087-28088	NN	denotes	#
T5892	28088-28093	CD	denotes	11995
T5893	28093-28094	HYPH	denotes	-
T5894	28094-28097	CD	denotes	065
T5895	28097-28098	-RRB-	denotes	]
T5896	28098-28100	,	denotes	,
T5897	28100-28102	CD	denotes	10
T5898	28102-28103	NN	denotes	%
T5899	28104-28109	JJ	denotes	fetal
T5901	28110-28116	JJ	denotes	bovine
T5900	28117-28122	NN	denotes	serum
T5902	28123-28124	-LRB-	denotes	[
T5903	28124-28129	NNP	denotes	Sigma
T5905	28130-28131	NN	denotes	#
T5904	28131-28132	NN	denotes	F
T5906	28132-28133	HYPH	denotes	-
T5907	28133-28137	CD	denotes	2442
T5908	28137-28138	-RRB-	denotes	]
T5909	28138-28140	,	denotes	,
T5910	28140-28152	VBN	denotes	supplemented
T5911	28153-28157	IN	denotes	with
T5912	28158-28160	CD	denotes	24
T5913	28161-28163	NN	denotes	mM
T5914	28164-28167	NN	denotes	KCl
T5915	28167-28168	-RRB-	denotes	)
T5916	28168-28169	.	denotes	.
T5917	28169-28382	sentence	denotes	Infection was performed the following day with defective retrovirus transducing the temperature-sensitive tsA58/U19 large T antigen and a selection neomycin-resistance cassette [27], as previously described [28].
T5918	28170-28179	NN	denotes	Infection
T5920	28180-28183	VBD	denotes	was
T5919	28184-28193	VBN	denotes	performed
T5921	28194-28197	DT	denotes	the
T5923	28198-28207	JJ	denotes	following
T5922	28208-28211	NN	denotes	day
T5924	28212-28216	IN	denotes	with
T5925	28217-28226	JJ	denotes	defective
T5926	28227-28237	NN	denotes	retrovirus
T5927	28238-28249	VBG	denotes	transducing
T5928	28250-28253	DT	denotes	the
T5930	28254-28265	NN	denotes	temperature
T5932	28265-28266	HYPH	denotes	-
T5931	28266-28275	JJ	denotes	sensitive
T5933	28276-28281	NN	denotes	tsA58
T5935	28281-28282	HYPH	denotes	/
T5934	28282-28285	NN	denotes	U19
T5936	28286-28291	JJ	denotes	large
T5937	28292-28293	NN	denotes	T
T5929	28294-28301	NN	denotes	antigen
T5938	28302-28305	CC	denotes	and
T5939	28306-28307	DT	denotes	a
T5941	28308-28317	NN	denotes	selection
T5942	28318-28326	NN	denotes	neomycin
T5944	28326-28327	HYPH	denotes	-
T5943	28327-28337	NN	denotes	resistance
T5940	28338-28346	NN	denotes	cassette
T5945	28347-28348	-LRB-	denotes	[
T5946	28348-28350	CD	denotes	27
T5947	28350-28351	-RRB-	denotes	]
T5948	28351-28353	,	denotes	,
T5949	28353-28355	IN	denotes	as
T5951	28356-28366	RB	denotes	previously
T5950	28367-28376	VBN	denotes	described
T5952	28377-28378	-LRB-	denotes	[
T5953	28378-28380	CD	denotes	28
T5954	28380-28381	-RRB-	denotes	]
T5955	28381-28382	.	denotes	.
T5956	28382-28554	sentence	denotes	Following infection, cultures were shifted to the tsA58/U19 permissive growth temperature of 33°C and selection was in the same growth media as above, with 200 μg/ml G418.
T5957	28383-28392	VBG	denotes	Following
T5959	28393-28402	NN	denotes	infection
T5960	28402-28404	,	denotes	,
T5961	28404-28412	NNS	denotes	cultures
T5962	28413-28417	VBD	denotes	were
T5958	28418-28425	VBN	denotes	shifted
T5963	28426-28428	IN	denotes	to
T5964	28429-28432	DT	denotes	the
T5966	28433-28438	NN	denotes	tsA58
T5968	28438-28439	HYPH	denotes	/
T5967	28439-28442	NN	denotes	U19
T5969	28443-28453	JJ	denotes	permissive
T5970	28454-28460	NN	denotes	growth
T5965	28461-28472	NN	denotes	temperature
T5971	28473-28475	IN	denotes	of
T5972	28476-28478	CD	denotes	33
T5973	28478-28480	NN	denotes	°C
T5974	28481-28484	CC	denotes	and
T5975	28485-28494	NN	denotes	selection
T5976	28495-28498	VBD	denotes	was
T5977	28499-28501	IN	denotes	in
T5978	28502-28505	DT	denotes	the
T5980	28506-28510	JJ	denotes	same
T5981	28511-28517	NN	denotes	growth
T5979	28518-28523	NNS	denotes	media
T5982	28524-28526	IN	denotes	as
T5983	28527-28532	RB	denotes	above
T5984	28532-28534	,	denotes	,
T5985	28534-28538	IN	denotes	with
T5986	28539-28542	CD	denotes	200
T5987	28543-28545	NN	denotes	μg
T5989	28545-28546	SYM	denotes	/
T5990	28546-28548	NN	denotes	ml
T5988	28549-28553	NN	denotes	G418
T5991	28553-28554	.	denotes	.
T5992	28554-28693	sentence	denotes	Conditionally immortalized colonies were isolated 3–9 weeks post-infection and expanded for frozen stocks and further sub-clone isolation.
T5993	28555-28568	RB	denotes	Conditionally
T5994	28569-28581	VBN	denotes	immortalized
T5995	28582-28590	NNS	denotes	colonies
T5997	28591-28595	VBD	denotes	were
T5996	28596-28604	VBN	denotes	isolated
T5998	28605-28606	CD	denotes	3
T6000	28606-28607	SYM	denotes	–
T5999	28607-28608	CD	denotes	9
T6001	28609-28614	NNS	denotes	weeks
T6002	28615-28629	RB	denotes	post-infection
T6003	28630-28633	CC	denotes	and
T6004	28634-28642	VBN	denotes	expanded
T6005	28643-28646	IN	denotes	for
T6006	28647-28653	JJ	denotes	frozen
T6007	28654-28660	NNS	denotes	stocks
T6008	28661-28664	CC	denotes	and
T6009	28665-28672	JJ	denotes	further
T6011	28673-28682	NN	denotes	sub-clone
T6010	28683-28692	NN	denotes	isolation
T6012	28692-28693	.	denotes	.
T6013	28693-28826	sentence	denotes	Multiple clonal lines were obtained for each genotype and all phenotypes were confirmed in at least 2 independent CbCln3 cell lines.
T6014	28694-28702	JJ	denotes	Multiple
T6016	28703-28709	JJ	denotes	clonal
T6015	28710-28715	NNS	denotes	lines
T6018	28716-28720	VBD	denotes	were
T6017	28721-28729	VBN	denotes	obtained
T6019	28730-28733	IN	denotes	for
T6020	28734-28738	DT	denotes	each
T6021	28739-28747	NN	denotes	genotype
T6022	28748-28751	CC	denotes	and
T6023	28752-28755	DT	denotes	all
T6024	28756-28766	NNS	denotes	phenotypes
T6025	28767-28771	VBD	denotes	were
T6026	28772-28781	VBN	denotes	confirmed
T6027	28782-28784	IN	denotes	in
T6028	28785-28787	RB	denotes	at
T6030	28788-28793	RBS	denotes	least
T6029	28794-28795	CD	denotes	2
T6032	28796-28807	JJ	denotes	independent
T6033	28808-28814	NN	denotes	CbCln3
T6034	28815-28819	NN	denotes	cell
T6031	28820-28825	NNS	denotes	lines
T6035	28825-28826	.	denotes	.
T6036	28826-28951	sentence	denotes	CbCln3 cell lines were maintained on 0.01% poly-ornithine coated dishes at 30–90% confluency, in 33°C and 5% CO2 atmosphere.
T6037	28827-28833	NN	denotes	CbCln3
T6039	28834-28838	NN	denotes	cell
T6038	28839-28844	NNS	denotes	lines
T6041	28845-28849	VBD	denotes	were
T6040	28850-28860	VBN	denotes	maintained
T6042	28861-28863	IN	denotes	on
T6043	28864-28868	CD	denotes	0.01
T6044	28868-28869	NN	denotes	%
T6046	28870-28874	NN	denotes	poly
T6048	28874-28875	HYPH	denotes	-
T6047	28875-28884	NN	denotes	ornithine
T6049	28885-28891	VBN	denotes	coated
T6045	28892-28898	NNS	denotes	dishes
T6050	28899-28901	IN	denotes	at
T6051	28902-28904	CD	denotes	30
T6053	28904-28905	SYM	denotes	–
T6052	28905-28907	CD	denotes	90
T6054	28907-28908	NN	denotes	%
T6055	28909-28919	NN	denotes	confluency
T6056	28919-28921	,	denotes	,
T6057	28921-28923	IN	denotes	in
T6058	28924-28926	CD	denotes	33
T6059	28926-28928	NN	denotes	°C
T6060	28929-28932	CC	denotes	and
T6061	28933-28934	CD	denotes	5
T6063	28934-28935	SYM	denotes	%
T6064	28936-28939	NN	denotes	CO2
T6062	28940-28950	NN	denotes	atmosphere
T6065	28950-28951	.	denotes	.
T6066	28951-29042	sentence	denotes	Passage number was recorded (up to ~20 passages), but had no apparent impact on phenotype.
T6067	28952-28959	NN	denotes	Passage
T6068	28960-28966	NN	denotes	number
T6070	28967-28970	VBD	denotes	was
T6069	28971-28979	VBN	denotes	recorded
T6071	28980-28981	-LRB-	denotes	(
T6073	28981-28983	IN	denotes	up
T6075	28984-28986	IN	denotes	to
T6076	28987-28988	SYM	denotes	~
T6074	28988-28990	CD	denotes	20
T6072	28991-28999	NNS	denotes	passages
T6077	28999-29000	-RRB-	denotes	)
T6078	29000-29002	,	denotes	,
T6079	29002-29005	CC	denotes	but
T6080	29006-29009	VBD	denotes	had
T6081	29010-29012	DT	denotes	no
T6083	29013-29021	JJ	denotes	apparent
T6082	29022-29028	NN	denotes	impact
T6084	29029-29031	IN	denotes	on
T6085	29032-29041	NN	denotes	phenotype
T6086	29041-29042	.	denotes	.
T6087	29042-29210	sentence	denotes	Neuronal differentiation was as previously described [29] with the following cocktail: 10 ng/ml α-FGF, 250 μM IBMX, 200 nM TPA, 50 μM forskolin, 5 μM dopamine (Sigma).
T6088	29043-29051	JJ	denotes	Neuronal
T6089	29052-29067	NN	denotes	differentiation
T6090	29068-29071	VBD	denotes	was
T6091	29072-29074	IN	denotes	as
T6093	29075-29085	RB	denotes	previously
T6092	29086-29095	VBN	denotes	described
T6094	29096-29097	-LRB-	denotes	[
T6095	29097-29099	CD	denotes	29
T6096	29099-29100	-RRB-	denotes	]
T6097	29101-29105	IN	denotes	with
T6098	29106-29109	DT	denotes	the
T6100	29110-29119	JJ	denotes	following
T6099	29120-29128	NN	denotes	cocktail
T6101	29128-29130	:	denotes	:
T6102	29130-29132	CD	denotes	10
T6103	29133-29135	NN	denotes	ng
T6105	29135-29136	SYM	denotes	/
T6106	29136-29138	NN	denotes	ml
T6107	29139-29140	NN	denotes	α
T6108	29140-29141	HYPH	denotes	-
T6104	29141-29144	NN	denotes	FGF
T6109	29144-29146	,	denotes	,
T6110	29146-29149	CD	denotes	250
T6111	29150-29152	NN	denotes	μM
T6112	29153-29157	NN	denotes	IBMX
T6113	29157-29159	,	denotes	,
T6114	29159-29162	CD	denotes	200
T6115	29163-29165	NN	denotes	nM
T6116	29166-29169	NN	denotes	TPA
T6117	29169-29171	,	denotes	,
T6118	29171-29173	CD	denotes	50
T6119	29174-29176	NN	denotes	μM
T6120	29177-29186	NN	denotes	forskolin
T6121	29186-29188	,	denotes	,
T6122	29188-29189	CD	denotes	5
T6123	29190-29192	NN	denotes	μM
T6124	29193-29201	NN	denotes	dopamine
T6125	29202-29203	-LRB-	denotes	(
T6126	29203-29208	NNP	denotes	Sigma
T6127	29208-29209	-RRB-	denotes	)
T6128	29209-29210	.	denotes	.
T6165	29212-29222	NN	denotes	Genotyping
T6166	29223-29226	CC	denotes	and
T6167	29227-29229	NN	denotes	RT
T6169	29229-29230	HYPH	denotes	-
T6168	29230-29233	NN	denotes	PCR
T6170	29233-29331	sentence	denotes	Genomic DNA was extracted from tail biopsies and cell pellets as described (Cotman et al., 2002).
T6171	29234-29241	JJ	denotes	Genomic
T6172	29242-29245	NN	denotes	DNA
T6174	29246-29249	VBD	denotes	was
T6173	29250-29259	VBN	denotes	extracted
T6175	29260-29264	IN	denotes	from
T6176	29265-29269	NN	denotes	tail
T6177	29270-29278	NNS	denotes	biopsies
T6178	29279-29282	CC	denotes	and
T6179	29283-29287	NN	denotes	cell
T6180	29288-29295	NNS	denotes	pellets
T6181	29296-29298	IN	denotes	as
T6182	29299-29308	VBN	denotes	described
T6183	29309-29310	-LRB-	denotes	(
T6184	29310-29316	NNP	denotes	Cotman
T6185	29317-29319	FW	denotes	et
T6186	29320-29323	FW	denotes	al.
T6187	29323-29325	,	denotes	,
T6188	29325-29329	CD	denotes	2002
T6189	29329-29330	-RRB-	denotes	)
T6190	29330-29331	.	denotes	.
T6191	29331-29620	sentence	denotes	Cln3Δex7/8 knock-in allele PCR genotyping was with wild-type primers, WtF (5'-CAGCATCTCCTCAGGGCTA-3') and WtR (5'-CCAACATAGAAAGTAGGGTGTGC-3') to yield a ~250 bp band and knock-in primers, 552F (5'-GAGCTTTGTTCTGGTTGCCTTC-3') and Ex9RA (5'-GCAGTCTCTGCCTCGTTTTCT-3') to yield a ~500 bp band.
T6192	29332-29340	NN	denotes	Cln3Δex7
T6194	29340-29341	HYPH	denotes	/
T6195	29341-29342	CD	denotes	8
T6196	29343-29348	VB	denotes	knock
T6197	29348-29349	HYPH	denotes	-
T6198	29349-29351	RP	denotes	in
T6193	29352-29358	NN	denotes	allele
T6200	29359-29362	NN	denotes	PCR
T6199	29363-29373	NN	denotes	genotyping
T6201	29374-29377	VBD	denotes	was
T6202	29378-29382	IN	denotes	with
T6203	29383-29387	JJ	denotes	wild
T6205	29387-29388	HYPH	denotes	-
T6204	29388-29392	NN	denotes	type
T6206	29393-29400	NNS	denotes	primers
T6207	29400-29402	,	denotes	,
T6208	29402-29405	NN	denotes	WtF
T6209	29406-29407	-LRB-	denotes	(
T6210	29407-29408	CD	denotes	5
T6212	29408-29409	SYM	denotes	'
T6213	29409-29410	HYPH	denotes	-
T6211	29410-29429	NN	denotes	CAGCATCTCCTCAGGGCTA
T6214	29429-29430	HYPH	denotes	-
T6215	29430-29431	CD	denotes	3
T6216	29431-29432	SYM	denotes	'
T6217	29432-29433	-RRB-	denotes	)
T6218	29434-29437	CC	denotes	and
T6219	29438-29441	NN	denotes	WtR
T6220	29442-29443	-LRB-	denotes	(
T6221	29443-29444	CD	denotes	5
T6223	29444-29445	SYM	denotes	'
T6224	29445-29446	HYPH	denotes	-
T6222	29446-29469	NN	denotes	CCAACATAGAAAGTAGGGTGTGC
T6225	29469-29470	HYPH	denotes	-
T6226	29470-29471	CD	denotes	3
T6227	29471-29472	SYM	denotes	'
T6228	29472-29473	-RRB-	denotes	)
T6229	29474-29476	TO	denotes	to
T6230	29477-29482	VB	denotes	yield
T6231	29483-29484	DT	denotes	a
T6233	29485-29486	SYM	denotes	~
T6234	29486-29489	CD	denotes	250
T6235	29490-29492	NN	denotes	bp
T6232	29493-29497	NN	denotes	band
T6236	29498-29501	CC	denotes	and
T6237	29502-29507	VB	denotes	knock
T6239	29507-29508	HYPH	denotes	-
T6240	29508-29510	RP	denotes	in
T6238	29511-29518	NNS	denotes	primers
T6241	29518-29520	,	denotes	,
T6242	29520-29524	NN	denotes	552F
T6243	29525-29526	-LRB-	denotes	(
T6244	29526-29527	CD	denotes	5
T6246	29527-29528	SYM	denotes	'
T6247	29528-29529	HYPH	denotes	-
T6245	29529-29551	NN	denotes	GAGCTTTGTTCTGGTTGCCTTC
T6248	29551-29552	HYPH	denotes	-
T6249	29552-29553	CD	denotes	3
T6250	29553-29554	SYM	denotes	'
T6251	29554-29555	-RRB-	denotes	)
T6252	29556-29559	CC	denotes	and
T6253	29560-29565	NN	denotes	Ex9RA
T6254	29566-29567	-LRB-	denotes	(
T6255	29567-29568	CD	denotes	5
T6257	29568-29569	SYM	denotes	'
T6258	29569-29570	HYPH	denotes	-
T6256	29570-29591	NN	denotes	GCAGTCTCTGCCTCGTTTTCT
T6259	29591-29592	HYPH	denotes	-
T6260	29592-29593	CD	denotes	3
T6261	29593-29594	SYM	denotes	'
T6262	29594-29595	-RRB-	denotes	)
T6263	29596-29598	TO	denotes	to
T6264	29599-29604	VB	denotes	yield
T6265	29605-29606	DT	denotes	a
T6267	29607-29608	SYM	denotes	~
T6268	29608-29611	CD	denotes	500
T6269	29612-29614	NN	denotes	bp
T6266	29615-29619	NN	denotes	band
T6270	29619-29620	.	denotes	.
T6271	29620-29739	sentence	denotes	PCR cycling conditions were 95°C for 30 seconds, 58°C for 30 seconds, and 72°C for 35 seconds, repeated for 34 cycles.
T6272	29621-29624	NN	denotes	PCR
T6273	29625-29632	NN	denotes	cycling
T6274	29633-29643	NNS	denotes	conditions
T6275	29644-29648	VBD	denotes	were
T6276	29649-29651	CD	denotes	95
T6277	29651-29653	NN	denotes	°C
T6278	29654-29657	IN	denotes	for
T6279	29658-29660	CD	denotes	30
T6280	29661-29668	NNS	denotes	seconds
T6281	29668-29670	,	denotes	,
T6282	29670-29672	CD	denotes	58
T6283	29672-29674	NN	denotes	°C
T6284	29675-29678	IN	denotes	for
T6285	29679-29681	CD	denotes	30
T6286	29682-29689	NNS	denotes	seconds
T6287	29689-29691	,	denotes	,
T6288	29691-29694	CC	denotes	and
T6289	29695-29697	CD	denotes	72
T6290	29697-29699	NN	denotes	°C
T6291	29700-29703	IN	denotes	for
T6292	29704-29706	CD	denotes	35
T6293	29707-29714	NNS	denotes	seconds
T6294	29714-29716	,	denotes	,
T6295	29716-29724	VBN	denotes	repeated
T6296	29725-29728	IN	denotes	for
T6297	29729-29731	CD	denotes	34
T6298	29732-29738	NNS	denotes	cycles
T6299	29738-29739	.	denotes	.
T6300	29739-29835	sentence	denotes	Total RNA isolation and Cln3 RT-PCR primers and procedures have been previously described [12].
T6301	29740-29745	JJ	denotes	Total
T6303	29746-29749	NN	denotes	RNA
T6302	29750-29759	NN	denotes	isolation
T6305	29760-29763	CC	denotes	and
T6306	29764-29768	NN	denotes	Cln3
T6308	29769-29771	NN	denotes	RT
T6310	29771-29772	HYPH	denotes	-
T6309	29772-29775	NN	denotes	PCR
T6307	29776-29783	NNS	denotes	primers
T6311	29784-29787	CC	denotes	and
T6312	29788-29798	NNS	denotes	procedures
T6313	29799-29803	VBP	denotes	have
T6314	29804-29808	VBN	denotes	been
T6315	29809-29819	RB	denotes	previously
T6304	29820-29829	VBN	denotes	described
T6316	29830-29831	-LRB-	denotes	[
T6317	29831-29833	CD	denotes	12
T6318	29833-29834	-RRB-	denotes	]
T6319	29834-29835	.	denotes	.
T6369	29837-29844	NN	denotes	Subunit
T6370	29845-29846	NN	denotes	c
T6371	29847-29859	NN	denotes	accumulation
T6372	29860-29865	NN	denotes	assay
T6373	29865-30076	sentence	denotes	Cells were seeded into 4-well chamber-slides (Falcon) at a density of 5 × 104 cells per well for microscopy studies, or into 100 mm dishes (Falcon) at a density of 5 × 105 cells per dish for protein extraction.
T6374	29866-29871	NNS	denotes	Cells
T6376	29872-29876	VBD	denotes	were
T6375	29877-29883	VBN	denotes	seeded
T6377	29884-29888	IN	denotes	into
T6378	29889-29890	CD	denotes	4
T6380	29890-29891	HYPH	denotes	-
T6379	29891-29895	NN	denotes	well
T6382	29896-29903	NN	denotes	chamber
T6383	29903-29904	HYPH	denotes	-
T6381	29904-29910	NNS	denotes	slides
T6384	29911-29912	-LRB-	denotes	(
T6385	29912-29918	NNP	denotes	Falcon
T6386	29918-29919	-RRB-	denotes	)
T6387	29920-29922	IN	denotes	at
T6388	29923-29924	DT	denotes	a
T6389	29925-29932	NN	denotes	density
T6390	29933-29935	IN	denotes	of
T6391	29936-29937	CD	denotes	5
T6393	29938-29939	SYM	denotes	×
T6392	29940-29943	CD	denotes	104
T6394	29944-29949	NNS	denotes	cells
T6395	29950-29953	IN	denotes	per
T6396	29954-29958	NN	denotes	well
T6397	29959-29962	IN	denotes	for
T6398	29963-29973	NN	denotes	microscopy
T6399	29974-29981	NNS	denotes	studies
T6400	29981-29983	,	denotes	,
T6401	29983-29985	CC	denotes	or
T6402	29986-29990	IN	denotes	into
T6403	29991-29994	CD	denotes	100
T6404	29995-29997	NN	denotes	mm
T6405	29998-30004	NNS	denotes	dishes
T6406	30005-30006	-LRB-	denotes	(
T6407	30006-30012	NNP	denotes	Falcon
T6408	30012-30013	-RRB-	denotes	)
T6409	30014-30016	IN	denotes	at
T6410	30017-30018	DT	denotes	a
T6411	30019-30026	NN	denotes	density
T6412	30027-30029	IN	denotes	of
T6413	30030-30031	CD	denotes	5
T6415	30032-30033	SYM	denotes	×
T6414	30034-30037	CD	denotes	105
T6416	30038-30043	NNS	denotes	cells
T6417	30044-30047	IN	denotes	per
T6418	30048-30052	NN	denotes	dish
T6419	30053-30056	IN	denotes	for
T6420	30057-30064	NN	denotes	protein
T6421	30065-30075	NN	denotes	extraction
T6422	30075-30076	.	denotes	.
T6423	30076-30194	sentence	denotes	Cells were typically >95% confluent one day post-plating, and the following day was considered 1-day post-confluency.
T6424	30077-30082	NNS	denotes	Cells
T6425	30083-30087	VBD	denotes	were
T6426	30088-30097	RB	denotes	typically
T6427	30098-30099	SYM	denotes	>
T6428	30099-30101	CD	denotes	95
T6429	30101-30102	NN	denotes	%
T6430	30103-30112	JJ	denotes	confluent
T6431	30113-30116	CD	denotes	one
T6432	30117-30120	NN	denotes	day
T6433	30121-30133	RB	denotes	post-plating
T6434	30133-30135	,	denotes	,
T6435	30135-30138	CC	denotes	and
T6436	30139-30142	DT	denotes	the
T6438	30143-30152	JJ	denotes	following
T6437	30153-30156	NN	denotes	day
T6440	30157-30160	VBD	denotes	was
T6439	30161-30171	VBN	denotes	considered
T6441	30172-30173	CD	denotes	1
T6443	30173-30174	HYPH	denotes	-
T6442	30174-30177	NN	denotes	day
T6444	30178-30193	RB	denotes	post-confluency
T6445	30193-30194	.	denotes	.
T6446	30194-30444	sentence	denotes	At the indicated times, cells were either fixed with 4% formaldehyde in phosphate buffered saline (PBS), pH 7.4, for 20 minutes and processed for autofluorescence/subunit c immunostaining, or cell pellets were collected for total protein extraction.
T6447	30195-30197	IN	denotes	At
T6449	30198-30201	DT	denotes	the
T6451	30202-30211	VBN	denotes	indicated
T6450	30212-30217	NNS	denotes	times
T6452	30217-30219	,	denotes	,
T6453	30219-30224	NNS	denotes	cells
T6454	30225-30229	VBD	denotes	were
T6455	30230-30236	RB	denotes	either
T6448	30237-30242	VBN	denotes	fixed
T6456	30243-30247	IN	denotes	with
T6457	30248-30249	CD	denotes	4
T6458	30249-30250	NN	denotes	%
T6459	30251-30263	NN	denotes	formaldehyde
T6460	30264-30266	IN	denotes	in
T6461	30267-30276	NN	denotes	phosphate
T6463	30277-30285	VBN	denotes	buffered
T6462	30286-30292	NN	denotes	saline
T6464	30293-30294	-LRB-	denotes	(
T6465	30294-30297	NN	denotes	PBS
T6466	30297-30298	-RRB-	denotes	)
T6467	30298-30300	,	denotes	,
T6468	30300-30302	NN	denotes	pH
T6469	30303-30306	CD	denotes	7.4
T6470	30306-30308	,	denotes	,
T6471	30308-30311	IN	denotes	for
T6472	30312-30314	CD	denotes	20
T6473	30315-30322	NNS	denotes	minutes
T6474	30323-30326	CC	denotes	and
T6475	30327-30336	VBN	denotes	processed
T6476	30337-30340	IN	denotes	for
T6477	30341-30357	NN	denotes	autofluorescence
T6478	30357-30358	HYPH	denotes	/
T6479	30358-30365	NN	denotes	subunit
T6480	30366-30367	NN	denotes	c
T6481	30368-30382	NN	denotes	immunostaining
T6482	30382-30384	,	denotes	,
T6483	30384-30386	CC	denotes	or
T6484	30387-30391	NN	denotes	cell
T6485	30392-30399	NNS	denotes	pellets
T6487	30400-30404	VBD	denotes	were
T6486	30405-30414	VBN	denotes	collected
T6488	30415-30418	IN	denotes	for
T6489	30419-30424	JJ	denotes	total
T6491	30425-30432	NN	denotes	protein
T6490	30433-30443	NN	denotes	extraction
T6492	30443-30444	.	denotes	.
T6493	30444-30645	sentence	denotes	Alternatively, cells were fixed with 2.5% glutaraldehyde/2% paraformaldehyde in 0.1 M cacodylate buffer, pH 7.4 for 1 hour and subsequently post-fixed and processed for TEM analysis as described [12].
T6494	30445-30458	RB	denotes	Alternatively
T6496	30458-30460	,	denotes	,
T6497	30460-30465	NNS	denotes	cells
T6498	30466-30470	VBD	denotes	were
T6495	30471-30476	VBN	denotes	fixed
T6499	30477-30481	IN	denotes	with
T6500	30482-30485	CD	denotes	2.5
T6501	30485-30486	NN	denotes	%
T6502	30487-30501	NN	denotes	glutaraldehyde
T6503	30501-30502	HYPH	denotes	/
T6504	30502-30503	CD	denotes	2
T6505	30503-30504	NN	denotes	%
T6506	30505-30521	NN	denotes	paraformaldehyde
T6507	30522-30524	IN	denotes	in
T6508	30525-30528	CD	denotes	0.1
T6509	30529-30530	NN	denotes	M
T6511	30531-30541	NN	denotes	cacodylate
T6510	30542-30548	NN	denotes	buffer
T6512	30548-30550	,	denotes	,
T6513	30550-30552	NN	denotes	pH
T6514	30553-30556	CD	denotes	7.4
T6515	30557-30560	IN	denotes	for
T6516	30561-30562	CD	denotes	1
T6517	30563-30567	NN	denotes	hour
T6518	30568-30571	CC	denotes	and
T6519	30572-30584	RB	denotes	subsequently
T6520	30585-30595	VBN	denotes	post-fixed
T6521	30596-30599	CC	denotes	and
T6522	30600-30609	VBN	denotes	processed
T6523	30610-30613	IN	denotes	for
T6524	30614-30617	NN	denotes	TEM
T6525	30618-30626	NN	denotes	analysis
T6526	30627-30629	IN	denotes	as
T6527	30630-30639	VBN	denotes	described
T6528	30640-30641	-LRB-	denotes	[
T6529	30641-30643	CD	denotes	12
T6530	30643-30644	-RRB-	denotes	]
T6531	30644-30645	.	denotes	.
T6532	30645-30740	sentence	denotes	In confocal microscopy studies, autofluorescent signal was observed over multiple wavelengths.
T6533	30646-30648	IN	denotes	In
T6535	30649-30657	JJ	denotes	confocal
T6537	30658-30668	NN	denotes	microscopy
T6536	30669-30676	NNS	denotes	studies
T6538	30676-30678	,	denotes	,
T6539	30678-30693	JJ	denotes	autofluorescent
T6540	30694-30700	NN	denotes	signal
T6541	30701-30704	VBD	denotes	was
T6534	30705-30713	VBN	denotes	observed
T6542	30714-30718	IN	denotes	over
T6543	30719-30727	JJ	denotes	multiple
T6544	30728-30739	NNS	denotes	wavelengths
T6545	30739-30740	.	denotes	.
T6546	30740-30857	sentence	denotes	For co-staining, settings were reduced such that autofluorescent signal did not contribute to antibody label signal.
T6547	30741-30744	IN	denotes	For
T6549	30745-30756	NN	denotes	co-staining
T6550	30756-30758	,	denotes	,
T6551	30758-30766	NNS	denotes	settings
T6552	30767-30771	VBD	denotes	were
T6548	30772-30779	VBN	denotes	reduced
T6553	30780-30784	JJ	denotes	such
T6554	30785-30789	IN	denotes	that
T6556	30790-30805	JJ	denotes	autofluorescent
T6557	30806-30812	NN	denotes	signal
T6558	30813-30816	VBD	denotes	did
T6559	30817-30820	RB	denotes	not
T6555	30821-30831	VB	denotes	contribute
T6560	30832-30834	IN	denotes	to
T6561	30835-30843	NN	denotes	antibody
T6563	30844-30849	NN	denotes	label
T6562	30850-30856	NN	denotes	signal
T6564	30856-30857	.	denotes	.
T6658	30859-30873	NN	denotes	Immunostaining
T6660	30874-30877	CC	denotes	and
T6661	30878-30888	NN	denotes	Immunoblot
T6659	30889-30897	NN	denotes	analysis
T6662	30897-31058	sentence	denotes	For immunostaining, cells were seeded at a density of 3–5 × 104 cells per well in 4-well chamber-slides and grown overnight at 33°C, unless indicated otherwise.
T6663	30898-30901	IN	denotes	For
T6665	30902-30916	NN	denotes	immunostaining
T6666	30916-30918	,	denotes	,
T6667	30918-30923	NNS	denotes	cells
T6668	30924-30928	VBD	denotes	were
T6664	30929-30935	VBN	denotes	seeded
T6669	30936-30938	IN	denotes	at
T6670	30939-30940	DT	denotes	a
T6671	30941-30948	NN	denotes	density
T6672	30949-30951	IN	denotes	of
T6673	30952-30953	CD	denotes	3
T6675	30953-30954	SYM	denotes	–
T6674	30954-30955	CD	denotes	5
T6677	30956-30957	SYM	denotes	×
T6676	30958-30961	CD	denotes	104
T6678	30962-30967	NNS	denotes	cells
T6679	30968-30971	IN	denotes	per
T6680	30972-30976	NN	denotes	well
T6681	30977-30979	IN	denotes	in
T6682	30980-30981	CD	denotes	4
T6684	30981-30982	HYPH	denotes	-
T6683	30982-30986	NN	denotes	well
T6686	30987-30994	NN	denotes	chamber
T6687	30994-30995	HYPH	denotes	-
T6685	30995-31001	NNS	denotes	slides
T6688	31002-31005	CC	denotes	and
T6689	31006-31011	VBN	denotes	grown
T6690	31012-31021	RB	denotes	overnight
T6691	31022-31024	IN	denotes	at
T6692	31025-31027	CD	denotes	33
T6693	31027-31029	NN	denotes	°C
T6694	31029-31031	,	denotes	,
T6695	31031-31037	IN	denotes	unless
T6696	31038-31047	VBN	denotes	indicated
T6697	31048-31057	RB	denotes	otherwise
T6698	31057-31058	.	denotes	.
T6699	31058-31242	sentence	denotes	Fixation was with ice-cold 4% formaldehyde in PBS, pH 7.4, for 20 minutes, or with ice-cold methanol/acetone (1:1) for 10 minutes at -20°C followed by air-drying (antibody-dependent).
T6700	31059-31067	NN	denotes	Fixation
T6701	31068-31071	VBD	denotes	was
T6702	31072-31076	IN	denotes	with
T6703	31077-31080	NN	denotes	ice
T6705	31080-31081	HYPH	denotes	-
T6704	31081-31085	JJ	denotes	cold
T6707	31086-31087	CD	denotes	4
T6708	31087-31088	NN	denotes	%
T6706	31089-31101	NN	denotes	formaldehyde
T6709	31102-31104	IN	denotes	in
T6710	31105-31108	NN	denotes	PBS
T6711	31108-31110	,	denotes	,
T6712	31110-31112	NN	denotes	pH
T6713	31113-31116	CD	denotes	7.4
T6714	31116-31118	,	denotes	,
T6715	31118-31121	IN	denotes	for
T6716	31122-31124	CD	denotes	20
T6717	31125-31132	NNS	denotes	minutes
T6718	31132-31134	,	denotes	,
T6719	31134-31136	CC	denotes	or
T6720	31137-31141	IN	denotes	with
T6721	31142-31145	NN	denotes	ice
T6723	31145-31146	HYPH	denotes	-
T6722	31146-31150	JJ	denotes	cold
T6725	31151-31159	NN	denotes	methanol
T6726	31159-31160	HYPH	denotes	/
T6724	31160-31167	NN	denotes	acetone
T6727	31168-31169	-LRB-	denotes	(
T6728	31169-31170	CD	denotes	1
T6729	31170-31171	SYM	denotes	:
T6730	31171-31172	CD	denotes	1
T6731	31172-31173	-RRB-	denotes	)
T6732	31174-31177	IN	denotes	for
T6733	31178-31180	CD	denotes	10
T6734	31181-31188	NNS	denotes	minutes
T6735	31189-31191	IN	denotes	at
T6736	31192-31193	SYM	denotes	-
T6737	31193-31195	CD	denotes	20
T6738	31195-31197	NN	denotes	°C
T6739	31198-31206	VBN	denotes	followed
T6740	31207-31209	IN	denotes	by
T6741	31210-31213	NN	denotes	air
T6743	31213-31214	HYPH	denotes	-
T6742	31214-31220	NN	denotes	drying
T6744	31221-31222	-LRB-	denotes	(
T6746	31222-31230	NN	denotes	antibody
T6747	31230-31231	HYPH	denotes	-
T6745	31231-31240	JJ	denotes	dependent
T6748	31240-31241	-RRB-	denotes	)
T6749	31241-31242	.	denotes	.
T6750	31242-31661	sentence	denotes	Cells were washed with PBS at least 2 times, 5 minutes per wash, between each of the following steps of the staining procedure: 0.1 M glycine in PBS for 5 minutes, 0.05% or 0.1% (antibody-dependent) Triton X-100 (Fisher Scientific) in PBS for 5 minutes, 2% bovine serum albumin (BSA) in PBS for 30 minutes, primary antibody diluted in 2% BSA/PBS for 90 minutes, secondary antibody diluted in 2% BSA/PBS for 60 minutes.
T6751	31243-31248	NNS	denotes	Cells
T6753	31249-31253	VBD	denotes	were
T6752	31254-31260	VBN	denotes	washed
T6754	31261-31265	IN	denotes	with
T6755	31266-31269	NN	denotes	PBS
T6756	31270-31272	RB	denotes	at
T6758	31273-31278	RBS	denotes	least
T6757	31279-31280	CD	denotes	2
T6759	31281-31286	NNS	denotes	times
T6760	31286-31288	,	denotes	,
T6761	31288-31289	CD	denotes	5
T6762	31290-31297	NNS	denotes	minutes
T6763	31298-31301	IN	denotes	per
T6764	31302-31306	NN	denotes	wash
T6765	31306-31308	,	denotes	,
T6766	31308-31315	IN	denotes	between
T6767	31316-31320	DT	denotes	each
T6768	31321-31323	IN	denotes	of
T6769	31324-31327	DT	denotes	the
T6771	31328-31337	JJ	denotes	following
T6770	31338-31343	NNS	denotes	steps
T6772	31344-31346	IN	denotes	of
T6773	31347-31350	DT	denotes	the
T6775	31351-31359	NN	denotes	staining
T6774	31360-31369	NN	denotes	procedure
T6776	31369-31371	:	denotes	:
T6777	31371-31374	CD	denotes	0.1
T6778	31375-31376	NN	denotes	M
T6779	31377-31384	NN	denotes	glycine
T6780	31385-31387	IN	denotes	in
T6781	31388-31391	NN	denotes	PBS
T6782	31392-31395	IN	denotes	for
T6783	31396-31397	CD	denotes	5
T6784	31398-31405	NNS	denotes	minutes
T6785	31405-31407	,	denotes	,
T6786	31407-31411	CD	denotes	0.05
T6787	31411-31412	NN	denotes	%
T6789	31413-31415	CC	denotes	or
T6790	31416-31419	CD	denotes	0.1
T6791	31419-31420	NN	denotes	%
T6792	31421-31422	-LRB-	denotes	(
T6793	31422-31430	NN	denotes	antibody
T6795	31430-31431	HYPH	denotes	-
T6794	31431-31440	JJ	denotes	dependent
T6796	31440-31441	-RRB-	denotes	)
T6797	31442-31448	NN	denotes	Triton
T6788	31449-31450	NN	denotes	X
T6798	31450-31451	HYPH	denotes	-
T6799	31451-31454	CD	denotes	100
T6800	31455-31456	-LRB-	denotes	(
T6802	31456-31462	NNP	denotes	Fisher
T6801	31463-31473	NNP	denotes	Scientific
T6803	31473-31474	-RRB-	denotes	)
T6804	31475-31477	IN	denotes	in
T6805	31478-31481	NN	denotes	PBS
T6806	31482-31485	IN	denotes	for
T6807	31486-31487	CD	denotes	5
T6808	31488-31495	NNS	denotes	minutes
T6809	31495-31497	,	denotes	,
T6810	31497-31498	CD	denotes	2
T6811	31498-31499	NN	denotes	%
T6812	31500-31506	JJ	denotes	bovine
T6814	31507-31512	NN	denotes	serum
T6813	31513-31520	NN	denotes	albumin
T6815	31521-31522	-LRB-	denotes	(
T6816	31522-31525	NN	denotes	BSA
T6817	31525-31526	-RRB-	denotes	)
T6818	31527-31529	IN	denotes	in
T6819	31530-31533	NN	denotes	PBS
T6820	31534-31537	IN	denotes	for
T6821	31538-31540	CD	denotes	30
T6822	31541-31548	NNS	denotes	minutes
T6823	31548-31550	,	denotes	,
T6824	31550-31557	JJ	denotes	primary
T6825	31558-31566	NN	denotes	antibody
T6826	31567-31574	VBN	denotes	diluted
T6827	31575-31577	IN	denotes	in
T6828	31578-31579	CD	denotes	2
T6829	31579-31580	NN	denotes	%
T6831	31581-31584	NN	denotes	BSA
T6832	31584-31585	HYPH	denotes	/
T6830	31585-31588	NN	denotes	PBS
T6833	31589-31592	IN	denotes	for
T6834	31593-31595	CD	denotes	90
T6835	31596-31603	NNS	denotes	minutes
T6836	31603-31605	,	denotes	,
T6837	31605-31614	JJ	denotes	secondary
T6838	31615-31623	NN	denotes	antibody
T6839	31624-31631	VBN	denotes	diluted
T6840	31632-31634	IN	denotes	in
T6841	31635-31636	CD	denotes	2
T6842	31636-31637	NN	denotes	%
T6844	31638-31641	NN	denotes	BSA
T6845	31641-31642	HYPH	denotes	/
T6843	31642-31645	NN	denotes	PBS
T6846	31646-31649	IN	denotes	for
T6847	31650-31652	CD	denotes	60
T6848	31653-31660	NNS	denotes	minutes
T6849	31660-31661	.	denotes	.
T6850	31661-31715	sentence	denotes	All incubations were carried out at room temperature.
T6851	31662-31665	DT	denotes	All
T6852	31666-31677	NNS	denotes	incubations
T6854	31678-31682	VBD	denotes	were
T6853	31683-31690	VBN	denotes	carried
T6855	31691-31694	RP	denotes	out
T6856	31695-31697	IN	denotes	at
T6857	31698-31702	NN	denotes	room
T6858	31703-31714	NN	denotes	temperature
T6859	31714-31715	.	denotes	.
T6860	31715-31966	sentence	denotes	Following staining procedures, slides were coverslipped with Vectashield mounting medium (Vector Laboratories) and analyzed on a BioRad Radiance 2100 confocal microscope (Biorad), with identical exposure settings for wild-type and mutant like images.
T6861	31716-31725	VBG	denotes	Following
T6863	31726-31734	NN	denotes	staining
T6864	31735-31745	NNS	denotes	procedures
T6865	31745-31747	,	denotes	,
T6866	31747-31753	NNS	denotes	slides
T6867	31754-31758	VBD	denotes	were
T6862	31759-31771	VBN	denotes	coverslipped
T6868	31772-31776	IN	denotes	with
T6869	31777-31788	NNP	denotes	Vectashield
T6871	31789-31797	NN	denotes	mounting
T6870	31798-31804	NN	denotes	medium
T6872	31805-31806	-LRB-	denotes	(
T6874	31806-31812	NNP	denotes	Vector
T6873	31813-31825	NNP	denotes	Laboratories
T6875	31825-31826	-RRB-	denotes	)
T6876	31827-31830	CC	denotes	and
T6877	31831-31839	VBN	denotes	analyzed
T6878	31840-31842	IN	denotes	on
T6879	31843-31844	DT	denotes	a
T6881	31845-31851	NNP	denotes	BioRad
T6882	31852-31860	NNP	denotes	Radiance
T6883	31861-31865	CD	denotes	2100
T6884	31866-31874	JJ	denotes	confocal
T6880	31875-31885	NN	denotes	microscope
T6885	31886-31887	-LRB-	denotes	(
T6886	31887-31893	NNP	denotes	Biorad
T6887	31893-31894	-RRB-	denotes	)
T6888	31894-31896	,	denotes	,
T6889	31896-31900	IN	denotes	with
T6890	31901-31910	JJ	denotes	identical
T6892	31911-31919	NN	denotes	exposure
T6891	31920-31928	NNS	denotes	settings
T6893	31929-31932	IN	denotes	for
T6894	31933-31937	JJ	denotes	wild
T6896	31937-31938	HYPH	denotes	-
T6895	31938-31942	NN	denotes	type
T6898	31943-31946	CC	denotes	and
T6899	31947-31953	JJ	denotes	mutant
T6900	31954-31958	JJ	denotes	like
T6897	31959-31965	NNS	denotes	images
T6901	31965-31966	.	denotes	.
T6902	31966-32101	sentence	denotes	All comparisons of wild-type and mutant staining were performed in Adobe Photoshop with identical brightness and contrast adjustments.
T6903	31967-31970	DT	denotes	All
T6904	31971-31982	NNS	denotes	comparisons
T6906	31983-31985	IN	denotes	of
T6907	31986-31990	JJ	denotes	wild
T6909	31990-31991	HYPH	denotes	-
T6908	31991-31995	NN	denotes	type
T6911	31996-31999	CC	denotes	and
T6912	32000-32006	NN	denotes	mutant
T6910	32007-32015	NN	denotes	staining
T6913	32016-32020	VBD	denotes	were
T6905	32021-32030	VBN	denotes	performed
T6914	32031-32033	IN	denotes	in
T6915	32034-32039	NNP	denotes	Adobe
T6916	32040-32049	NNP	denotes	Photoshop
T6917	32050-32054	IN	denotes	with
T6918	32055-32064	JJ	denotes	identical
T6920	32065-32075	NN	denotes	brightness
T6921	32076-32079	CC	denotes	and
T6922	32080-32088	NN	denotes	contrast
T6919	32089-32100	NNS	denotes	adjustments
T6923	32100-32101	.	denotes	.
T6924	32101-32364	sentence	denotes	Total proteins were isolated from cell pellets by extraction with ice-cold 20 mM Tris, pH 7.4, 1% Triton X-100 (membrane-research grade, Roche), plus protease inhibitors (Complete mini tablet, 0.7 μg/ml pepstatin A, 2 μg/ml aprotinin, 5 μg/ml leupeptin [Roche]).
T6925	32102-32107	JJ	denotes	Total
T6926	32108-32116	NN	denotes	proteins
T6928	32117-32121	VBD	denotes	were
T6927	32122-32130	VBN	denotes	isolated
T6929	32131-32135	IN	denotes	from
T6930	32136-32140	NN	denotes	cell
T6931	32141-32148	NNS	denotes	pellets
T6932	32149-32151	IN	denotes	by
T6933	32152-32162	NN	denotes	extraction
T6934	32163-32167	IN	denotes	with
T6935	32168-32171	NN	denotes	ice
T6937	32171-32172	HYPH	denotes	-
T6938	32172-32176	JJ	denotes	cold
T6939	32177-32179	CD	denotes	20
T6940	32180-32182	NN	denotes	mM
T6936	32183-32187	NN	denotes	Tris
T6941	32187-32189	,	denotes	,
T6942	32189-32191	NN	denotes	pH
T6943	32192-32195	CD	denotes	7.4
T6944	32195-32197	,	denotes	,
T6945	32197-32198	CD	denotes	1
T6946	32198-32199	NN	denotes	%
T6948	32200-32206	NN	denotes	Triton
T6947	32207-32208	NN	denotes	X
T6949	32208-32209	HYPH	denotes	-
T6950	32209-32212	CD	denotes	100
T6951	32213-32214	-LRB-	denotes	(
T6953	32214-32222	NN	denotes	membrane
T6955	32222-32223	HYPH	denotes	-
T6954	32223-32231	NN	denotes	research
T6956	32232-32237	NN	denotes	grade
T6957	32237-32239	,	denotes	,
T6952	32239-32244	NNP	denotes	Roche
T6958	32244-32245	-RRB-	denotes	)
T6959	32245-32247	,	denotes	,
T6960	32247-32251	CC	denotes	plus
T6961	32252-32260	NN	denotes	protease
T6962	32261-32271	NNS	denotes	inhibitors
T6963	32272-32273	-LRB-	denotes	(
T6964	32273-32281	JJ	denotes	Complete
T6966	32282-32286	NN	denotes	mini
T6965	32287-32293	NN	denotes	tablet
T6967	32293-32295	,	denotes	,
T6968	32295-32298	CD	denotes	0.7
T6969	32299-32301	NN	denotes	μg
T6971	32301-32302	SYM	denotes	/
T6972	32302-32304	NN	denotes	ml
T6973	32305-32314	NN	denotes	pepstatin
T6970	32315-32316	NN	denotes	A
T6974	32316-32318	,	denotes	,
T6975	32318-32319	CD	denotes	2
T6976	32320-32322	NN	denotes	μg
T6978	32322-32323	SYM	denotes	/
T6979	32323-32325	NN	denotes	ml
T6977	32326-32335	NN	denotes	aprotinin
T6980	32335-32337	,	denotes	,
T6981	32337-32338	CD	denotes	5
T6982	32339-32341	NN	denotes	μg
T6984	32341-32342	SYM	denotes	/
T6985	32342-32344	NN	denotes	ml
T6983	32345-32354	NN	denotes	leupeptin
T6986	32355-32356	-LRB-	denotes	[
T6987	32356-32361	NNP	denotes	Roche
T6988	32361-32362	-RRB-	denotes	]
T6989	32362-32363	-RRB-	denotes	)
T6990	32363-32364	.	denotes	.
T6991	32364-32509	sentence	denotes	Following homogenization through a 25-gauge needle (~10 passes), extracts were centrifuged at 1000 × g for 10 minutes, at 4°C, to remove debris.
T6992	32365-32374	VBG	denotes	Following
T6994	32375-32389	NN	denotes	homogenization
T6995	32390-32397	IN	denotes	through
T6996	32398-32399	DT	denotes	a
T6998	32400-32402	CD	denotes	25
T7000	32402-32403	HYPH	denotes	-
T6999	32403-32408	NN	denotes	gauge
T6997	32409-32415	NN	denotes	needle
T7001	32416-32417	-LRB-	denotes	(
T7003	32417-32418	SYM	denotes	~
T7004	32418-32420	CD	denotes	10
T7002	32421-32427	NNS	denotes	passes
T7005	32427-32428	-RRB-	denotes	)
T7006	32428-32430	,	denotes	,
T7007	32430-32438	NNS	denotes	extracts
T7008	32439-32443	VBD	denotes	were
T6993	32444-32455	VBN	denotes	centrifuged
T7009	32456-32458	IN	denotes	at
T7010	32459-32463	CD	denotes	1000
T7012	32464-32465	SYM	denotes	×
T7011	32466-32467	NN	denotes	g
T7013	32468-32471	IN	denotes	for
T7014	32472-32474	CD	denotes	10
T7015	32475-32482	NNS	denotes	minutes
T7016	32482-32484	,	denotes	,
T7017	32484-32486	IN	denotes	at
T7018	32487-32488	CD	denotes	4
T7019	32488-32490	NN	denotes	°C
T7020	32490-32492	,	denotes	,
T7021	32492-32494	TO	denotes	to
T7022	32495-32501	VB	denotes	remove
T7023	32502-32508	NN	denotes	debris
T7024	32508-32509	.	denotes	.
T7025	32509-32658	sentence	denotes	Typically, 20–40 μg of protein (determined by Bio-rad Dc Protein Assay) was separated by SDS-PAGE, for subsequent immunoblotting, as described [12].
T7026	32510-32519	RB	denotes	Typically
T7028	32519-32521	,	denotes	,
T7029	32521-32523	CD	denotes	20
T7031	32523-32524	SYM	denotes	–
T7030	32524-32526	CD	denotes	40
T7032	32527-32529	NN	denotes	μg
T7033	32530-32532	IN	denotes	of
T7034	32533-32540	NN	denotes	protein
T7035	32541-32542	-LRB-	denotes	(
T7036	32542-32552	VBN	denotes	determined
T7037	32553-32555	IN	denotes	by
T7038	32556-32559	NNP	denotes	Bio
T7040	32559-32560	HYPH	denotes	-
T7039	32560-32563	NNP	denotes	rad
T7042	32564-32566	NN	denotes	Dc
T7043	32567-32574	NN	denotes	Protein
T7041	32575-32580	NN	denotes	Assay
T7044	32580-32581	-RRB-	denotes	)
T7045	32582-32585	VBD	denotes	was
T7027	32586-32595	VBN	denotes	separated
T7046	32596-32598	IN	denotes	by
T7047	32599-32602	NN	denotes	SDS
T7049	32602-32603	HYPH	denotes	-
T7048	32603-32607	NN	denotes	PAGE
T7050	32607-32609	,	denotes	,
T7051	32609-32612	IN	denotes	for
T7052	32613-32623	JJ	denotes	subsequent
T7053	32624-32638	NN	denotes	immunoblotting
T7054	32638-32640	,	denotes	,
T7055	32640-32642	IN	denotes	as
T7056	32643-32652	VBN	denotes	described
T7057	32653-32654	-LRB-	denotes	[
T7058	32654-32656	CD	denotes	12
T7059	32656-32657	-RRB-	denotes	]
T7060	32657-32658	.	denotes	.
T7061	32658-32743	sentence	denotes	16.5% tris-tricine SDS-PAGE gels were used for subunit c immunoblotting experiments.
T7062	32659-32663	CD	denotes	16.5
T7063	32663-32664	NN	denotes	%
T7065	32665-32669	NN	denotes	tris
T7067	32669-32670	HYPH	denotes	-
T7066	32670-32677	NN	denotes	tricine
T7068	32678-32681	NN	denotes	SDS
T7070	32681-32682	HYPH	denotes	-
T7069	32682-32686	NN	denotes	PAGE
T7064	32687-32691	NNS	denotes	gels
T7072	32692-32696	VBD	denotes	were
T7071	32697-32701	VBN	denotes	used
T7073	32702-32705	IN	denotes	for
T7074	32706-32713	NN	denotes	subunit
T7075	32714-32715	NN	denotes	c
T7077	32716-32730	NN	denotes	immunoblotting
T7076	32731-32742	NNS	denotes	experiments
T7078	32742-32743	.	denotes	.
T7116	32745-32754	NN	denotes	Cathepsin
T7117	32755-32756	NN	denotes	D
T7119	32757-32765	NN	denotes	activity
T7118	32766-32771	NN	denotes	assay
T7120	32771-33032	sentence	denotes	100 mm tissue culture dishes, which were approximately 80–90% confluent, were washed briefly with ice-cold PBS, and total protein extracts were isolated by scraping cells into 10 mM Tris, pH 7.4, 0.1% Triton X-100 followed by incubation on ice, for 20 minutes.
T7121	32772-32775	CD	denotes	100
T7122	32776-32778	NN	denotes	mm
T7124	32779-32785	NN	denotes	tissue
T7125	32786-32793	NN	denotes	culture
T7123	32794-32800	NNS	denotes	dishes
T7127	32800-32802	,	denotes	,
T7128	32802-32807	WDT	denotes	which
T7129	32808-32812	VBD	denotes	were
T7130	32813-32826	RB	denotes	approximately
T7132	32827-32829	CD	denotes	80
T7133	32829-32830	SYM	denotes	–
T7131	32830-32832	CD	denotes	90
T7134	32832-32833	NN	denotes	%
T7135	32834-32843	JJ	denotes	confluent
T7136	32843-32845	,	denotes	,
T7137	32845-32849	VBD	denotes	were
T7126	32850-32856	VBN	denotes	washed
T7138	32857-32864	RB	denotes	briefly
T7139	32865-32869	IN	denotes	with
T7140	32870-32873	NN	denotes	ice
T7142	32873-32874	HYPH	denotes	-
T7141	32874-32878	JJ	denotes	cold
T7143	32879-32882	NN	denotes	PBS
T7144	32882-32884	,	denotes	,
T7145	32884-32887	CC	denotes	and
T7146	32888-32893	JJ	denotes	total
T7148	32894-32901	NN	denotes	protein
T7147	32902-32910	NNS	denotes	extracts
T7150	32911-32915	VBD	denotes	were
T7149	32916-32924	VBN	denotes	isolated
T7151	32925-32927	IN	denotes	by
T7152	32928-32936	VBG	denotes	scraping
T7153	32937-32942	NNS	denotes	cells
T7154	32943-32947	IN	denotes	into
T7155	32948-32950	CD	denotes	10
T7156	32951-32953	NN	denotes	mM
T7157	32954-32958	NN	denotes	Tris
T7158	32958-32960	,	denotes	,
T7159	32960-32962	NN	denotes	pH
T7160	32963-32966	CD	denotes	7.4
T7161	32966-32968	,	denotes	,
T7162	32968-32971	CD	denotes	0.1
T7163	32971-32972	NN	denotes	%
T7165	32973-32979	NN	denotes	Triton
T7164	32980-32981	NN	denotes	X
T7166	32981-32982	HYPH	denotes	-
T7167	32982-32985	CD	denotes	100
T7168	32986-32994	VBN	denotes	followed
T7169	32995-32997	IN	denotes	by
T7170	32998-33008	NN	denotes	incubation
T7171	33009-33011	IN	denotes	on
T7172	33012-33015	NN	denotes	ice
T7173	33015-33017	,	denotes	,
T7174	33017-33020	IN	denotes	for
T7175	33021-33023	CD	denotes	20
T7176	33024-33031	NNS	denotes	minutes
T7177	33031-33032	.	denotes	.
T7178	33032-33191	sentence	denotes	The insoluble material was centrifuged at 14,000 g, the supernatant was isolated, and protein concentration was determined using the Bio-rad Dc Protein Assay.
T7179	33033-33036	DT	denotes	The
T7181	33037-33046	JJ	denotes	insoluble
T7180	33047-33055	NN	denotes	material
T7183	33056-33059	VBD	denotes	was
T7182	33060-33071	VBN	denotes	centrifuged
T7184	33072-33074	IN	denotes	at
T7185	33075-33081	CD	denotes	14,000
T7186	33082-33083	NN	denotes	g
T7187	33083-33085	,	denotes	,
T7188	33085-33088	DT	denotes	the
T7189	33089-33100	NN	denotes	supernatant
T7191	33101-33104	VBD	denotes	was
T7190	33105-33113	VBN	denotes	isolated
T7192	33113-33115	,	denotes	,
T7193	33115-33118	CC	denotes	and
T7194	33119-33126	NN	denotes	protein
T7195	33127-33140	NN	denotes	concentration
T7197	33141-33144	VBD	denotes	was
T7196	33145-33155	VBN	denotes	determined
T7198	33156-33161	VBG	denotes	using
T7199	33162-33165	DT	denotes	the
T7201	33166-33169	NNP	denotes	Bio
T7203	33169-33170	HYPH	denotes	-
T7202	33170-33173	NNP	denotes	rad
T7204	33174-33176	NN	denotes	Dc
T7205	33177-33184	NN	denotes	Protein
T7200	33185-33190	NN	denotes	Assay
T7206	33190-33191	.	denotes	.
T7207	33191-33411	sentence	denotes	50–70 μg of total protein extract were used to measure cathepsin D activity using the Fluorogenic Innozyme™ Cathepsin D Immunocapture Activity Assay Kit (EMD Biosciences) according to the manufacturer's recommendations.
T7208	33192-33194	CD	denotes	50
T7210	33194-33195	SYM	denotes	–
T7209	33195-33197	CD	denotes	70
T7211	33198-33200	NN	denotes	μg
T7213	33201-33203	IN	denotes	of
T7214	33204-33209	JJ	denotes	total
T7216	33210-33217	NN	denotes	protein
T7215	33218-33225	NN	denotes	extract
T7217	33226-33230	VBD	denotes	were
T7212	33231-33235	VBN	denotes	used
T7218	33236-33238	TO	denotes	to
T7219	33239-33246	VB	denotes	measure
T7220	33247-33256	NN	denotes	cathepsin
T7221	33257-33258	NN	denotes	D
T7222	33259-33267	NN	denotes	activity
T7223	33268-33273	VBG	denotes	using
T7224	33274-33277	DT	denotes	the
T7226	33278-33289	JJ	denotes	Fluorogenic
T7228	33290-33298	NNP	denotes	Innozyme
T7229	33298-33299	SYM	denotes	™
T7230	33300-33309	NN	denotes	Cathepsin
T7227	33310-33311	NN	denotes	D
T7231	33312-33325	NN	denotes	Immunocapture
T7232	33326-33334	NN	denotes	Activity
T7233	33335-33340	NN	denotes	Assay
T7225	33341-33344	NN	denotes	Kit
T7234	33345-33346	-LRB-	denotes	(
T7236	33346-33349	NNP	denotes	EMD
T7235	33350-33361	NNP	denotes	Biosciences
T7237	33361-33362	-RRB-	denotes	)
T7238	33363-33372	VBG	denotes	according
T7239	33373-33375	IN	denotes	to
T7240	33376-33379	DT	denotes	the
T7241	33380-33392	NN	denotes	manufacturer
T7243	33392-33394	POS	denotes	's
T7242	33395-33410	NNS	denotes	recommendations
T7244	33410-33411	.	denotes	.
T7245	33411-33685	sentence	denotes	Relative fluorescence was measured using an Analyst AD plate reader (Molecular Devices) with the following filters and settings: excitation filter, 360-35; emission filter, 400-20, Flash lamp with 100 readings/well, 100 ms between readings, and 100,000 μs integration time.
T7246	33412-33420	JJ	denotes	Relative
T7247	33421-33433	NN	denotes	fluorescence
T7249	33434-33437	VBD	denotes	was
T7248	33438-33446	VBN	denotes	measured
T7250	33447-33452	VBG	denotes	using
T7251	33453-33455	DT	denotes	an
T7253	33456-33463	NN	denotes	Analyst
T7254	33464-33466	NN	denotes	AD
T7255	33467-33472	NN	denotes	plate
T7252	33473-33479	NN	denotes	reader
T7256	33480-33481	-LRB-	denotes	(
T7258	33481-33490	JJ	denotes	Molecular
T7257	33491-33498	NNPS	denotes	Devices
T7259	33498-33499	-RRB-	denotes	)
T7260	33500-33504	IN	denotes	with
T7261	33505-33508	DT	denotes	the
T7263	33509-33518	JJ	denotes	following
T7262	33519-33526	NNS	denotes	filters
T7264	33527-33530	CC	denotes	and
T7265	33531-33539	NNS	denotes	settings
T7266	33539-33541	:	denotes	:
T7267	33541-33551	NN	denotes	excitation
T7268	33552-33558	NN	denotes	filter
T7270	33558-33560	,	denotes	,
T7271	33560-33563	CD	denotes	360
T7272	33563-33564	HYPH	denotes	-
T7269	33564-33566	CD	denotes	35
T7273	33566-33567	:	denotes	;
T7274	33568-33576	NN	denotes	emission
T7275	33577-33583	NN	denotes	filter
T7277	33583-33585	,	denotes	,
T7278	33585-33588	CD	denotes	400
T7279	33588-33589	HYPH	denotes	-
T7276	33589-33591	CD	denotes	20
T7280	33591-33593	,	denotes	,
T7281	33593-33598	NN	denotes	Flash
T7282	33599-33603	NN	denotes	lamp
T7283	33604-33608	IN	denotes	with
T7284	33609-33612	CD	denotes	100
T7285	33613-33621	NNS	denotes	readings
T7286	33621-33622	SYM	denotes	/
T7287	33622-33626	NN	denotes	well
T7288	33626-33628	,	denotes	,
T7289	33628-33631	CD	denotes	100
T7290	33632-33634	NN	denotes	ms
T7291	33635-33642	IN	denotes	between
T7292	33643-33651	NNS	denotes	readings
T7293	33651-33653	,	denotes	,
T7294	33653-33656	CC	denotes	and
T7295	33657-33664	CD	denotes	100,000
T7296	33665-33667	NN	denotes	μs
T7298	33668-33679	NN	denotes	integration
T7297	33680-33684	NN	denotes	time
T7299	33684-33685	.	denotes	.
T7337	33687-33696	JJ	denotes	Lysosomal
T7338	33697-33705	NN	denotes	staining
T7339	33706-33709	CC	denotes	and
T7340	33710-33719	JJ	denotes	endocytic
T7341	33720-33726	NN	denotes	uptake
T7342	33726-33839	sentence	denotes	Cells were seeded at a density of 3–5 × 104 cells per well in 4-well chamber-slides and grown overnight at 33°C.
T7343	33727-33732	NNS	denotes	Cells
T7345	33733-33737	VBD	denotes	were
T7344	33738-33744	VBN	denotes	seeded
T7346	33745-33747	IN	denotes	at
T7347	33748-33749	DT	denotes	a
T7348	33750-33757	NN	denotes	density
T7349	33758-33760	IN	denotes	of
T7350	33761-33762	CD	denotes	3
T7352	33762-33763	SYM	denotes	–
T7351	33763-33764	CD	denotes	5
T7354	33765-33766	SYM	denotes	×
T7353	33767-33770	CD	denotes	104
T7355	33771-33776	NNS	denotes	cells
T7356	33777-33780	IN	denotes	per
T7357	33781-33785	NN	denotes	well
T7358	33786-33788	IN	denotes	in
T7359	33789-33790	CD	denotes	4
T7361	33790-33791	HYPH	denotes	-
T7360	33791-33795	NN	denotes	well
T7363	33796-33803	NN	denotes	chamber
T7364	33803-33804	HYPH	denotes	-
T7362	33804-33810	NNS	denotes	slides
T7365	33811-33814	CC	denotes	and
T7366	33815-33820	VBN	denotes	grown
T7367	33821-33830	RB	denotes	overnight
T7368	33831-33833	IN	denotes	at
T7369	33834-33836	CD	denotes	33
T7370	33836-33838	NN	denotes	°C
T7371	33838-33839	.	denotes	.
T7372	33839-34033	sentence	denotes	Growth media was exchanged for fresh, pre-warmed growth media containing 500 nM Lysotracker or 1 mg/ml dextran-FITC, and cells were incubated at 33°C for 45 minutes or 15 minutes, respectively.
T7373	33840-33846	NN	denotes	Growth
T7374	33847-33852	NNS	denotes	media
T7376	33853-33856	VBD	denotes	was
T7375	33857-33866	VBN	denotes	exchanged
T7377	33867-33870	IN	denotes	for
T7378	33871-33876	JJ	denotes	fresh
T7380	33876-33878	,	denotes	,
T7381	33878-33888	VBN	denotes	pre-warmed
T7382	33889-33895	NN	denotes	growth
T7379	33896-33901	NNS	denotes	media
T7383	33902-33912	VBG	denotes	containing
T7384	33913-33916	CD	denotes	500
T7385	33917-33919	NN	denotes	nM
T7386	33920-33931	NNP	denotes	Lysotracker
T7387	33932-33934	CC	denotes	or
T7388	33935-33936	CD	denotes	1
T7389	33937-33939	NN	denotes	mg
T7391	33939-33940	SYM	denotes	/
T7392	33940-33942	NN	denotes	ml
T7393	33943-33950	NN	denotes	dextran
T7394	33950-33951	HYPH	denotes	-
T7390	33951-33955	NN	denotes	FITC
T7395	33955-33957	,	denotes	,
T7396	33957-33960	CC	denotes	and
T7397	33961-33966	NNS	denotes	cells
T7399	33967-33971	VBD	denotes	were
T7398	33972-33981	VBN	denotes	incubated
T7400	33982-33984	IN	denotes	at
T7401	33985-33987	CD	denotes	33
T7402	33987-33989	NN	denotes	°C
T7403	33990-33993	IN	denotes	for
T7404	33994-33996	CD	denotes	45
T7405	33997-34004	NNS	denotes	minutes
T7406	34005-34007	CC	denotes	or
T7407	34008-34010	CD	denotes	15
T7408	34011-34018	NNS	denotes	minutes
T7409	34018-34020	,	denotes	,
T7410	34020-34032	RB	denotes	respectively
T7411	34032-34033	.	denotes	.
T7412	34033-34206	sentence	denotes	Following labeling, cells were immediately placed on ice and washed for 10 minutes in ice-cold dye-free media, and fixed with 4% formaldehyde in PBS, for 20 minutes on ice.
T7413	34034-34043	VBG	denotes	Following
T7415	34044-34052	NN	denotes	labeling
T7416	34052-34054	,	denotes	,
T7417	34054-34059	NNS	denotes	cells
T7418	34060-34064	VBD	denotes	were
T7419	34065-34076	RB	denotes	immediately
T7414	34077-34083	VBN	denotes	placed
T7420	34084-34086	IN	denotes	on
T7421	34087-34090	NN	denotes	ice
T7422	34091-34094	CC	denotes	and
T7423	34095-34101	VBN	denotes	washed
T7424	34102-34105	IN	denotes	for
T7425	34106-34108	CD	denotes	10
T7426	34109-34116	NNS	denotes	minutes
T7427	34117-34119	IN	denotes	in
T7428	34120-34123	NN	denotes	ice
T7430	34123-34124	HYPH	denotes	-
T7429	34124-34128	JJ	denotes	cold
T7432	34129-34132	NN	denotes	dye
T7434	34132-34133	HYPH	denotes	-
T7433	34133-34137	JJ	denotes	free
T7431	34138-34143	NNS	denotes	media
T7435	34143-34145	,	denotes	,
T7436	34145-34148	CC	denotes	and
T7437	34149-34154	VBN	denotes	fixed
T7438	34155-34159	IN	denotes	with
T7439	34160-34161	CD	denotes	4
T7440	34161-34162	NN	denotes	%
T7441	34163-34175	NN	denotes	formaldehyde
T7442	34176-34178	IN	denotes	in
T7443	34179-34182	NN	denotes	PBS
T7444	34182-34184	,	denotes	,
T7445	34184-34187	IN	denotes	for
T7446	34188-34190	CD	denotes	20
T7447	34191-34198	NNS	denotes	minutes
T7448	34199-34201	IN	denotes	on
T7449	34202-34205	NN	denotes	ice
T7450	34205-34206	.	denotes	.
T7451	34206-34343	sentence	denotes	Chambers were removed and slides were coverslipped with Vectashield mounting media for confocal microscopy analysis, as described above.
T7452	34207-34215	NNS	denotes	Chambers
T7454	34216-34220	VBD	denotes	were
T7453	34221-34228	VBN	denotes	removed
T7455	34229-34232	CC	denotes	and
T7456	34233-34239	NNS	denotes	slides
T7458	34240-34244	VBD	denotes	were
T7457	34245-34257	VBN	denotes	coverslipped
T7459	34258-34262	IN	denotes	with
T7460	34263-34274	NNP	denotes	Vectashield
T7462	34275-34283	NN	denotes	mounting
T7461	34284-34289	NNS	denotes	media
T7463	34290-34293	IN	denotes	for
T7464	34294-34302	JJ	denotes	confocal
T7465	34303-34313	NN	denotes	microscopy
T7466	34314-34322	NN	denotes	analysis
T7467	34322-34324	,	denotes	,
T7468	34324-34326	IN	denotes	as
T7469	34327-34336	VBN	denotes	described
T7470	34337-34342	RB	denotes	above
T7471	34342-34343	.	denotes	.
T7484	34345-34357	JJ	denotes	Morphometric
T7485	34358-34366	NN	denotes	analysis
T7486	34367-34369	IN	denotes	of
T7487	34370-34382	NNS	denotes	mitochondria
T7488	34382-34475	sentence	denotes	TEM photomicrographs (10,000 × – 40,000 × magnification) were taken from random grid fields.
T7489	34383-34386	NN	denotes	TEM
T7490	34387-34403	NNS	denotes	photomicrographs
T7492	34404-34405	-LRB-	denotes	(
T7494	34405-34411	CD	denotes	10,000
T7496	34412-34413	SYM	denotes	×
T7497	34414-34415	SYM	denotes	–
T7495	34416-34422	CD	denotes	40,000
T7498	34423-34424	SYM	denotes	×
T7493	34425-34438	NN	denotes	magnification
T7499	34438-34439	-RRB-	denotes	)
T7500	34440-34444	VBD	denotes	were
T7491	34445-34450	VBN	denotes	taken
T7501	34451-34455	IN	denotes	from
T7502	34456-34462	JJ	denotes	random
T7504	34463-34467	NN	denotes	grid
T7503	34468-34474	NNS	denotes	fields
T7505	34474-34475	.	denotes	.
T7506	34475-34712	sentence	denotes	For length measurements, the longest side of each mitochondria was measured in centimeters, and along the length of the mitochondria, width measurements were taken every 2.5–4 mm (dependent on the magnification of the micrograph image).
T7507	34476-34479	IN	denotes	For
T7509	34480-34486	NN	denotes	length
T7510	34487-34499	NNS	denotes	measurements
T7511	34499-34501	,	denotes	,
T7512	34501-34504	DT	denotes	the
T7514	34505-34512	JJS	denotes	longest
T7513	34513-34517	NN	denotes	side
T7515	34518-34520	IN	denotes	of
T7516	34521-34525	DT	denotes	each
T7517	34526-34538	NNS	denotes	mitochondria
T7518	34539-34542	VBD	denotes	was
T7508	34543-34551	VBN	denotes	measured
T7519	34552-34554	IN	denotes	in
T7520	34555-34566	NNS	denotes	centimeters
T7521	34566-34568	,	denotes	,
T7522	34568-34571	CC	denotes	and
T7523	34572-34577	IN	denotes	along
T7525	34578-34581	DT	denotes	the
T7526	34582-34588	NN	denotes	length
T7527	34589-34591	IN	denotes	of
T7528	34592-34595	DT	denotes	the
T7529	34596-34608	NNS	denotes	mitochondria
T7530	34608-34610	,	denotes	,
T7531	34610-34615	NN	denotes	width
T7532	34616-34628	NNS	denotes	measurements
T7533	34629-34633	VBD	denotes	were
T7524	34634-34639	VBN	denotes	taken
T7534	34640-34645	DT	denotes	every
T7536	34646-34649	CD	denotes	2.5
T7538	34649-34650	SYM	denotes	–
T7537	34650-34651	CD	denotes	4
T7535	34652-34654	NN	denotes	mm
T7539	34655-34656	-LRB-	denotes	(
T7540	34656-34665	JJ	denotes	dependent
T7541	34666-34668	IN	denotes	on
T7542	34669-34672	DT	denotes	the
T7543	34673-34686	NN	denotes	magnification
T7544	34687-34689	IN	denotes	of
T7545	34690-34693	DT	denotes	the
T7547	34694-34704	NN	denotes	micrograph
T7546	34705-34710	NN	denotes	image
T7548	34710-34711	-RRB-	denotes	)
T7549	34711-34712	.	denotes	.
T7550	34712-34846	sentence	denotes	Following measurement, all numbers were normalized to reflect one magnification and data was analyzed using Microsoft Excel software.
T7551	34713-34722	VBG	denotes	Following
T7553	34723-34734	NN	denotes	measurement
T7554	34734-34736	,	denotes	,
T7555	34736-34739	DT	denotes	all
T7556	34740-34747	NNS	denotes	numbers
T7557	34748-34752	VBD	denotes	were
T7552	34753-34763	VBN	denotes	normalized
T7558	34764-34766	TO	denotes	to
T7559	34767-34774	VB	denotes	reflect
T7560	34775-34778	CD	denotes	one
T7561	34779-34792	NN	denotes	magnification
T7562	34793-34796	CC	denotes	and
T7563	34797-34801	NNS	denotes	data
T7565	34802-34805	VBD	denotes	was
T7564	34806-34814	VBN	denotes	analyzed
T7566	34815-34820	VBG	denotes	using
T7567	34821-34830	NNP	denotes	Microsoft
T7568	34831-34836	NNP	denotes	Excel
T7569	34837-34845	NN	denotes	software
T7570	34845-34846	.	denotes	.
T7571	34846-34998	sentence	denotes	To ascertain unmagnified mitochondrial size, final measurement data, in centimeters, was converted to nanometers according to scale bar representation.
T7572	34847-34849	TO	denotes	To
T7573	34850-34859	VB	denotes	ascertain
T7575	34860-34871	JJ	denotes	unmagnified
T7577	34872-34885	JJ	denotes	mitochondrial
T7576	34886-34890	NN	denotes	size
T7578	34890-34892	,	denotes	,
T7579	34892-34897	JJ	denotes	final
T7581	34898-34909	NN	denotes	measurement
T7580	34910-34914	NNS	denotes	data
T7582	34914-34916	,	denotes	,
T7583	34916-34918	IN	denotes	in
T7584	34919-34930	NNS	denotes	centimeters
T7585	34930-34932	,	denotes	,
T7586	34932-34935	VBD	denotes	was
T7574	34936-34945	VBN	denotes	converted
T7587	34946-34948	IN	denotes	to
T7588	34949-34959	NNS	denotes	nanometers
T7589	34960-34969	VBG	denotes	according
T7590	34970-34972	IN	denotes	to
T7591	34973-34978	NN	denotes	scale
T7592	34979-34982	NN	denotes	bar
T7593	34983-34997	NN	denotes	representation
T7594	34997-34998	.	denotes	.
T7619	35000-35003	NN	denotes	ATP
T7620	35004-35015	NN	denotes	measurement
T7621	35015-35151	sentence	denotes	ATP was measured by using the CellTiter-GLO® Luminescent Cell Viability kit (Promega), according to the manufacturer's recommendations.
T7622	35016-35019	NN	denotes	ATP
T7624	35020-35023	VBD	denotes	was
T7623	35024-35032	VBN	denotes	measured
T7625	35033-35035	IN	denotes	by
T7626	35036-35041	VBG	denotes	using
T7627	35042-35045	DT	denotes	the
T7629	35046-35055	NNP	denotes	CellTiter
T7631	35055-35056	HYPH	denotes	-
T7630	35056-35060	NNP	denotes	GLO®
T7632	35061-35072	JJ	denotes	Luminescent
T7633	35073-35077	NN	denotes	Cell
T7634	35078-35087	NN	denotes	Viability
T7628	35088-35091	NN	denotes	kit
T7635	35092-35093	-LRB-	denotes	(
T7636	35093-35100	NNP	denotes	Promega
T7637	35100-35101	-RRB-	denotes	)
T7638	35101-35103	,	denotes	,
T7639	35103-35112	VBG	denotes	according
T7640	35113-35115	IN	denotes	to
T7641	35116-35119	DT	denotes	the
T7642	35120-35132	NN	denotes	manufacturer
T7644	35132-35134	POS	denotes	's
T7643	35135-35150	NNS	denotes	recommendations
T7645	35150-35151	.	denotes	.
T7646	35151-35298	sentence	denotes	Briefly, cells were plated in a black opaque-walled 96 well plate (Packard Bioscience) at a density of 20000/well and incubated at 33°C overnight.
T7647	35152-35159	RB	denotes	Briefly
T7649	35159-35161	,	denotes	,
T7650	35161-35166	NNS	denotes	cells
T7651	35167-35171	VBD	denotes	were
T7648	35172-35178	VBN	denotes	plated
T7652	35179-35181	IN	denotes	in
T7653	35182-35183	DT	denotes	a
T7655	35184-35189	JJ	denotes	black
T7656	35190-35196	JJ	denotes	opaque
T7658	35196-35197	HYPH	denotes	-
T7657	35197-35203	JJ	denotes	walled
T7659	35204-35206	CD	denotes	96
T7660	35207-35211	NN	denotes	well
T7654	35212-35217	NN	denotes	plate
T7661	35218-35219	-LRB-	denotes	(
T7663	35219-35226	NNP	denotes	Packard
T7662	35227-35237	NNP	denotes	Bioscience
T7664	35237-35238	-RRB-	denotes	)
T7665	35239-35241	IN	denotes	at
T7666	35242-35243	DT	denotes	a
T7667	35244-35251	NN	denotes	density
T7668	35252-35254	IN	denotes	of
T7669	35255-35260	CD	denotes	20000
T7670	35260-35261	SYM	denotes	/
T7671	35261-35265	NN	denotes	well
T7672	35266-35269	CC	denotes	and
T7673	35270-35279	VBN	denotes	incubated
T7674	35280-35282	IN	denotes	at
T7675	35283-35285	CD	denotes	33
T7676	35285-35287	NN	denotes	°C
T7677	35288-35297	RB	denotes	overnight
T7678	35297-35298	.	denotes	.
T7679	35298-35411	sentence	denotes	The following day, CellTiter-GLO® Reagent was added to each well and cell lysis was induced by mixing 2 minutes.
T7680	35299-35302	DT	denotes	The
T7682	35303-35312	JJ	denotes	following
T7681	35313-35316	NN	denotes	day
T7684	35316-35318	,	denotes	,
T7685	35318-35327	NNP	denotes	CellTiter
T7687	35327-35328	HYPH	denotes	-
T7686	35328-35332	NNP	denotes	GLO®
T7688	35333-35340	NN	denotes	Reagent
T7689	35341-35344	VBD	denotes	was
T7683	35345-35350	VBN	denotes	added
T7690	35351-35353	IN	denotes	to
T7691	35354-35358	DT	denotes	each
T7692	35359-35363	NN	denotes	well
T7693	35364-35367	CC	denotes	and
T7694	35368-35372	NN	denotes	cell
T7695	35373-35378	NN	denotes	lysis
T7697	35379-35382	VBD	denotes	was
T7696	35383-35390	VBN	denotes	induced
T7698	35391-35393	IN	denotes	by
T7699	35394-35400	VBG	denotes	mixing
T7700	35401-35402	CD	denotes	2
T7701	35403-35410	NNS	denotes	minutes
T7702	35410-35411	.	denotes	.
T7703	35411-35465	sentence	denotes	An ATP standard curve was prepared in the same plate.
T7704	35412-35414	DT	denotes	An
T7706	35415-35418	NN	denotes	ATP
T7707	35419-35427	JJ	denotes	standard
T7705	35428-35433	NN	denotes	curve
T7709	35434-35437	VBD	denotes	was
T7708	35438-35446	VBN	denotes	prepared
T7710	35447-35449	IN	denotes	in
T7711	35450-35453	DT	denotes	the
T7713	35454-35458	JJ	denotes	same
T7712	35459-35464	NN	denotes	plate
T7714	35464-35465	.	denotes	.
T7715	35465-35675	sentence	denotes	Before recording luminescence with a microplate luminometer (MicroLumat Plus LB 96V, Berthold Techonologies), the plate was dark adapted for 10 minutes at room temperature to stabilize the luminescence signal.
T7716	35466-35472	IN	denotes	Before
T7718	35473-35482	VBG	denotes	recording
T7719	35483-35495	NN	denotes	luminescence
T7720	35496-35500	IN	denotes	with
T7721	35501-35502	DT	denotes	a
T7723	35503-35513	NN	denotes	microplate
T7722	35514-35525	NN	denotes	luminometer
T7724	35526-35527	-LRB-	denotes	(
T7725	35527-35537	NNP	denotes	MicroLumat
T7727	35538-35542	NNP	denotes	Plus
T7728	35543-35545	NNP	denotes	LB
T7726	35546-35549	NNP	denotes	96V
T7729	35549-35551	,	denotes	,
T7731	35551-35559	NNP	denotes	Berthold
T7730	35560-35573	NNP	denotes	Techonologies
T7732	35573-35574	-RRB-	denotes	)
T7733	35574-35576	,	denotes	,
T7734	35576-35579	DT	denotes	the
T7735	35580-35585	NN	denotes	plate
T7736	35586-35589	VBD	denotes	was
T7737	35590-35594	NN	denotes	dark
T7717	35595-35602	VBN	denotes	adapted
T7738	35603-35606	IN	denotes	for
T7739	35607-35609	CD	denotes	10
T7740	35610-35617	NNS	denotes	minutes
T7741	35618-35620	IN	denotes	at
T7742	35621-35625	NN	denotes	room
T7743	35626-35637	NN	denotes	temperature
T7744	35638-35640	TO	denotes	to
T7745	35641-35650	VB	denotes	stabilize
T7746	35651-35654	DT	denotes	the
T7748	35655-35667	NN	denotes	luminescence
T7747	35668-35674	NN	denotes	signal
T7749	35674-35675	.	denotes	.
T7780	35677-35685	NN	denotes	Hydrogen
T7781	35686-35694	NN	denotes	peroxide
T7782	35695-35704	NN	denotes	treatment
T7783	35705-35710	NN	denotes	assay
T7784	35710-35813	sentence	denotes	Cells were plated at a density of 10,000 cells/well in 96-well plates and incubated at 33°C overnight.
T7785	35711-35716	NNS	denotes	Cells
T7787	35717-35721	VBD	denotes	were
T7786	35722-35728	VBN	denotes	plated
T7788	35729-35731	IN	denotes	at
T7789	35732-35733	DT	denotes	a
T7790	35734-35741	NN	denotes	density
T7791	35742-35744	IN	denotes	of
T7792	35745-35751	CD	denotes	10,000
T7793	35752-35757	NNS	denotes	cells
T7794	35757-35758	SYM	denotes	/
T7795	35758-35762	NN	denotes	well
T7796	35763-35765	IN	denotes	in
T7797	35766-35768	CD	denotes	96
T7799	35768-35769	HYPH	denotes	-
T7798	35769-35773	NN	denotes	well
T7800	35774-35780	NNS	denotes	plates
T7801	35781-35784	CC	denotes	and
T7802	35785-35794	VBN	denotes	incubated
T7803	35795-35797	IN	denotes	at
T7804	35798-35800	CD	denotes	33
T7805	35800-35802	NN	denotes	°C
T7806	35803-35812	RB	denotes	overnight
T7807	35812-35813	.	denotes	.
T7808	35813-35927	sentence	denotes	The following day, fresh media containing varying concentrations of hydrogen peroxide was dispersed to each well.
T7809	35814-35817	DT	denotes	The
T7811	35818-35827	VBG	denotes	following
T7810	35828-35831	NN	denotes	day
T7813	35831-35833	,	denotes	,
T7814	35833-35838	JJ	denotes	fresh
T7815	35839-35844	NNS	denotes	media
T7816	35845-35855	VBG	denotes	containing
T7817	35856-35863	VBG	denotes	varying
T7818	35864-35878	NNS	denotes	concentrations
T7819	35879-35881	IN	denotes	of
T7820	35882-35890	NN	denotes	hydrogen
T7821	35891-35899	NN	denotes	peroxide
T7822	35900-35903	VBD	denotes	was
T7812	35904-35913	VBN	denotes	dispersed
T7823	35914-35916	IN	denotes	to
T7824	35917-35921	DT	denotes	each
T7825	35922-35926	NN	denotes	well
T7826	35926-35927	.	denotes	.
T7827	35927-36167	sentence	denotes	Cells were incubated in the presence of hydrogen peroxide for 24 hours, at 33°C, and viability was measured using the CellTiter-96® AQueous Non-Radioactive Cell Proliferation Assay (Promega), according to the manufacturer's specifications.
T7828	35928-35933	NNS	denotes	Cells
T7830	35934-35938	VBD	denotes	were
T7829	35939-35948	VBN	denotes	incubated
T7831	35949-35951	IN	denotes	in
T7832	35952-35955	DT	denotes	the
T7833	35956-35964	NN	denotes	presence
T7834	35965-35967	IN	denotes	of
T7835	35968-35976	NN	denotes	hydrogen
T7836	35977-35985	NN	denotes	peroxide
T7837	35986-35989	IN	denotes	for
T7838	35990-35992	CD	denotes	24
T7839	35993-35998	NNS	denotes	hours
T7840	35998-36000	,	denotes	,
T7841	36000-36002	IN	denotes	at
T7842	36003-36005	CD	denotes	33
T7843	36005-36007	NN	denotes	°C
T7844	36007-36009	,	denotes	,
T7845	36009-36012	CC	denotes	and
T7846	36013-36022	NN	denotes	viability
T7848	36023-36026	VBD	denotes	was
T7847	36027-36035	VBN	denotes	measured
T7849	36036-36041	VBG	denotes	using
T7850	36042-36045	DT	denotes	the
T7852	36046-36055	NNP	denotes	CellTiter
T7854	36055-36056	HYPH	denotes	-
T7853	36056-36059	NN	denotes	96®
T7855	36060-36067	JJ	denotes	AQueous
T7856	36068-36083	JJ	denotes	Non-Radioactive
T7857	36084-36088	NN	denotes	Cell
T7858	36089-36102	NN	denotes	Proliferation
T7851	36103-36108	NN	denotes	Assay
T7859	36109-36110	-LRB-	denotes	(
T7860	36110-36117	NNP	denotes	Promega
T7861	36117-36118	-RRB-	denotes	)
T7862	36118-36120	,	denotes	,
T7863	36120-36129	VBG	denotes	according
T7864	36130-36132	IN	denotes	to
T7865	36133-36136	DT	denotes	the
T7866	36137-36149	NN	denotes	manufacturer
T7868	36149-36151	POS	denotes	's
T7867	36152-36166	NNS	denotes	specifications
T7869	36166-36167	.	denotes	.
R10	T179	T173	dobj	deposition,precede
R100	T283	T281	compound	JNCL,mutation
R1000	T1871	T1872	compound	Cln3,isoforms
R1001	T1872	T1866	nsubj	isoforms,were
R1002	T1873	T1872	compound	mRNA,isoforms
R1003	T1874	T1872	prep	in,isoforms
R1004	T1875	T1876	amod	wild,type
R1005	T1876	T1878	nmod	type,cells
R1006	T1877	T1876	punct	-,type
R1007	T1878	T1874	pobj	cells,in
R1008	T1879	T1876	cc	and,type
R1009	T1880	T1876	conj	homozygous,type
R101	T284	T278	prep	in,consequences
R1010	T1881	T1866	acomp	similar,were
R1011	T1882	T1881	prep	to,similar
R1012	T1883	T1882	pobj	those,to
R1013	T1884	T1883	acl	observed,those
R1014	T1885	T1884	prep	in,observed
R1015	T1886	T1887	amod	total,RNA
R1016	T1887	T1885	pobj	RNA,in
R1017	T1888	T1887	acl	isolated,RNA
R1018	T1889	T1888	prep	from,isolated
R1019	T1890	T1891	amod	wild,type
R102	T285	T286	amod	neuronal,cells
R1020	T1891	T1893	nmod	type,brain
R1021	T1892	T1891	punct	-,type
R1022	T1893	T1889	pobj	brain,from
R1023	T1894	T1891	cc	or,type
R1024	T1895	T1891	conj	homozygous,type
R1025	T1896	T1893	nmod	Cln3Δex7,brain
R1026	T1897	T1896	punct	/,Cln3Δex7
R1027	T1898	T1896	nummod	8,Cln3Δex7
R1028	T1899	T1893	amod	knock,brain
R1029	T1900	T1899	punct	-,knock
R103	T286	T284	pobj	cells,in
R1030	T1901	T1899	prt	in,knock
R1031	T1902	T1866	punct	", ",were
R1032	T1903	T1866	advmod	respectively,were
R1033	T1904	T1905	punct	(,Fig.
R1034	T1905	T1866	parataxis	Fig.,were
R1035	T1906	T1905	nummod	2,Fig.
R1036	T1907	T1905	punct	),Fig.
R1037	T1908	T1866	punct	.,were
R1038	T1910	T1911	prep	In,detected
R1039	T1912	T1910	pobj	addition,In
R104	T287	T276	punct	", ",used
R1040	T1913	T1911	punct	", ",detected
R1041	T1914	T1915	compound	batp1,immunostaining
R1042	T1915	T1911	nsubj	immunostaining,detected
R1043	T1916	T1917	compound	mutant,product
R1044	T1917	T1911	dobj	product,detected
R1045	T1918	T1917	compound	battenin,product
R1046	T1919	T1917	prep	in,product
R1047	T1920	T1921	amod	homozygous,cells
R1048	T1921	T1919	pobj	cells,in
R1049	T1922	T1921	nmod	CbCln3Δex7,cells
R105	T288	T276	nsubj	we,used
R1050	T1923	T1922	punct	/,CbCln3Δex7
R1051	T1924	T1922	nummod	8,CbCln3Δex7
R1052	T1925	T1917	punct	", ",product
R1053	T1926	T1917	prep	in,product
R1054	T1927	T1928	det	a,pattern
R1055	T1928	T1926	pobj	pattern,in
R1056	T1929	T1928	amod	similar,pattern
R1057	T1930	T1931	mark	albeit,reduced
R1058	T1931	T1929	parataxis	reduced,similar
R1059	T1932	T1928	amod	cytoplasmic,pattern
R106	T289	T290	nmod	P4,cerebella
R1060	T1933	T1928	punct	", ",pattern
R1061	T1934	T1928	amod	vesicular,pattern
R1062	T1935	T1928	compound	staining,pattern
R1063	T1936	T1928	prep	as,pattern
R1064	T1937	T1936	pobj	that,as
R1065	T1938	T1937	acl	seen,that
R1066	T1939	T1938	prep	in,seen
R1067	T1940	T1941	amod	wild,type
R1068	T1941	T1943	compound	type,cells
R1069	T1942	T1941	punct	-,type
R107	T290	T276	dobj	cerebella,used
R1070	T1943	T1939	pobj	cells,in
R1071	T1944	T1911	punct	.,detected
R1072	T1946	T1947	compound	Batp1,signal
R1073	T1947	T1948	nsubj	signal,exhibited
R1074	T1949	T1950	det	some,overlap
R1075	T1950	T1948	dobj	overlap,exhibited
R1076	T1951	T1948	prep	with,exhibited
R1077	T1952	T1953	det	the,marker
R1078	T1953	T1951	pobj	marker,with
R1079	T1954	T1953	amod	lysosomal,marker
R108	T291	T290	amod	knock,cerebella
R1080	T1955	T1953	punct	", ",marker
R1081	T1956	T1953	appos	Lamp1,marker
R1082	T1957	T1948	punct	", ",exhibited
R1083	T1958	T1948	cc	but,exhibited
R1084	T1959	T1948	conj	had,exhibited
R1085	T1960	T1961	advmod	more,significant
R1086	T1961	T1962	amod	significant,overlap
R1087	T1962	T1959	dobj	overlap,had
R1088	T1963	T1959	prep	with,had
R1089	T1964	T1965	amod	early,antigen
R109	T292	T291	punct	-,knock
R1090	T1965	T1963	pobj	antigen,with
R1091	T1966	T1965	compound	endosome,antigen
R1092	T1967	T1965	nummod	1,antigen
R1093	T1968	T1965	punct	(,antigen
R1094	T1969	T1965	appos	EEA1,antigen
R1095	T1970	T1965	punct	),antigen
R1096	T1971	T1965	cc	and,antigen
R1097	T1972	T1973	det	the,marker
R1098	T1973	T1965	conj	marker,antigen
R1099	T1974	T1973	amod	late,marker
R11	T180	T173	prep	in,precede
R110	T293	T291	prt	in,knock
R1100	T1975	T1973	amod	endosomal,marker
R1101	T1976	T1973	punct	", ",marker
R1102	T1977	T1973	appos	Rab7,marker
R1103	T1978	T1979	punct	(,Fig.
R1104	T1979	T1959	parataxis	Fig.,had
R1105	T1980	T1979	nummod	3,Fig.
R1106	T1981	T1979	punct	),Fig.
R1107	T1982	T1948	punct	.,exhibited
R1108	T1984	T1985	advmod	Only,overlap
R1109	T1985	T1987	nsubjpass	overlap,observed
R111	T294	T290	compound	mouse,cerebella
R1110	T1986	T1985	amod	limited,overlap
R1111	T1988	T1987	auxpass	was,observed
R1112	T1989	T1987	prep	with,observed
R1113	T1990	T1991	amod	recycling,endosomes
R1114	T1991	T1989	pobj	endosomes,with
R1115	T1992	T1987	punct	", ",observed
R1116	T1993	T1994	mark	as,determined
R1117	T1994	T1987	advcl	determined,observed
R1118	T1995	T1994	agent	by,determined
R1119	T1996	T1997	compound	transferrin,receptor
R112	T295	T296	aux	to,establish
R1120	T1997	T1998	compound	receptor,co-staining
R1121	T1998	T1995	pobj	co-staining,by
R1122	T1999	T2000	punct	(,shown
R1123	T2000	T1987	parataxis	shown,observed
R1124	T2001	T2000	nsubj	data,shown
R1125	T2002	T2000	neg	not,shown
R1126	T2003	T2000	punct	),shown
R1127	T2004	T1987	punct	.,observed
R1128	T2006	T2007	advmod	Intriguingly,altered
R1129	T2008	T2007	punct	", ",altered
R113	T296	T276	advcl	establish,used
R1130	T2009	T2010	nmod	Lamp1,distribution
R1131	T2010	T2007	nsubjpass	distribution,altered
R1132	T2011	T2009	cc	and,Lamp1
R1133	T2012	T2009	conj	EEA1,Lamp1
R1134	T2013	T2010	amod	immunocytochemical,distribution
R1135	T2014	T2007	auxpass	were,altered
R1136	T2015	T2007	prep	in,altered
R1137	T2016	T2017	amod	homozygous,cells
R1138	T2017	T2015	pobj	cells,in
R1139	T2018	T2017	nmod	CbCln3Δex7,cells
R114	T297	T298	advmod	conditionally,immortalized
R1140	T2019	T2018	punct	/,CbCln3Δex7
R1141	T2020	T2018	nummod	8,CbCln3Δex7
R1142	T2021	T2007	punct	", ",altered
R1143	T2022	T2007	prep	with,altered
R1144	T2023	T2024	amod	less,clustering
R1145	T2024	T2022	pobj	clustering,with
R1146	T2025	T2024	amod	perinuclear,clustering
R1147	T2026	T2024	prep	than,clustering
R1148	T2027	T2026	prep	in,than
R1149	T2028	T2029	amod	wild,type
R115	T298	T299	amod	immortalized,lines
R1150	T2029	T2031	compound	type,cells
R1151	T2030	T2029	punct	-,type
R1152	T2031	T2027	pobj	cells,in
R1153	T2032	T2007	punct	", ",altered
R1154	T2033	T2007	cc	and,altered
R1155	T2034	T2035	compound	Rab7,staining
R1156	T2035	T2036	nsubj	staining,was
R1157	T2036	T2007	conj	was,altered
R1158	T2037	T2036	advmod	frequently,was
R1159	T2038	T2039	advmod	less,intense
R116	T299	T296	dobj	lines,establish
R1160	T2039	T2036	acomp	intense,was
R1161	T2040	T2039	prep	in,intense
R1162	T2041	T2042	amod	homozygous,cells
R1163	T2042	T2040	pobj	cells,in
R1164	T2043	T2042	nmod	CbCln3Δex7,cells
R1165	T2044	T2043	punct	/,CbCln3Δex7
R1166	T2045	T2043	nummod	8,CbCln3Δex7
R1167	T2046	T2047	punct	(,Fig.
R1168	T2047	T2036	parataxis	Fig.,was
R1169	T2048	T2047	nummod	3,Fig.
R117	T300	T299	nmod	CbCln3,lines
R1170	T2049	T2047	punct	),Fig.
R1171	T2050	T2036	punct	.,was
R1172	T2052	T2053	amod	Heterozygous,cells
R1173	T2053	T2057	nsubj	cells,contained
R1174	T2054	T2053	nmod	CbCln3Δex7,cells
R1175	T2055	T2054	punct	/,CbCln3Δex7
R1176	T2056	T2054	nummod	8,CbCln3Δex7
R1177	T2058	T2059	det	a,mixture
R1178	T2059	T2057	dobj	mixture,contained
R1179	T2060	T2059	prep	of,mixture
R118	T301	T302	amod	wild,type
R1180	T2061	T2062	compound	Cln3,products
R1181	T2062	T2060	pobj	products,of
R1182	T2063	T2062	compound	mRNA,products
R1183	T2064	T2059	prep	from,mixture
R1184	T2065	T2066	preconj	both,allele
R1185	T2066	T2064	pobj	allele,from
R1186	T2067	T2066	det	the,allele
R1187	T2068	T2069	amod	wild,type
R1188	T2069	T2066	compound	type,allele
R1189	T2070	T2069	punct	-,type
R119	T302	T299	nmod	type,lines
R1190	T2071	T2066	cc	and,allele
R1191	T2072	T2073	det	the,allele
R1192	T2073	T2066	conj	allele,allele
R1193	T2074	T2073	compound	mutant,allele
R1194	T2075	T2057	punct	", ",contained
R1195	T2076	T2057	cc	and,contained
R1196	T2077	T2078	compound	batp1,signal
R1197	T2078	T2079	nsubj	signal,was
R1198	T2079	T2057	conj	was,contained
R1199	T2080	T2079	acomp	similar,was
R12	T181	T182	det	a,model
R120	T303	T302	punct	-,type
R1200	T2081	T2080	prep	to,similar
R1201	T2082	T2081	pobj	that,to
R1202	T2083	T2082	acl	seen,that
R1203	T2084	T2083	prep	in,seen
R1204	T2085	T2086	amod	wild,type
R1205	T2086	T2088	compound	type,cells
R1206	T2087	T2086	punct	-,type
R1207	T2088	T2084	pobj	cells,in
R1208	T2089	T2090	punct	(,shown
R1209	T2090	T2079	parataxis	shown,was
R121	T304	T302	punct	", ",type
R1210	T2091	T2090	nsubj	data,shown
R1211	T2092	T2090	neg	not,shown
R1212	T2093	T2090	punct	),shown
R1213	T2094	T2079	punct	.,was
R1214	T2096	T2097	prep	During,displayed
R1215	T2098	T2099	amod	sub-confluent,conditions
R1216	T2099	T2096	pobj	conditions,During
R1217	T2100	T2099	compound	growth,conditions
R1218	T2101	T2097	punct	", ",displayed
R1219	T2102	T2103	preconj	neither,type
R122	T305	T302	conj	heterozygous,type
R1220	T2103	T2106	nmod	type,cells
R1221	T2104	T2103	amod	wild,type
R1222	T2105	T2103	punct	-,type
R1223	T2106	T2097	nsubj	cells,displayed
R1224	T2107	T2103	cc	nor,type
R1225	T2108	T2109	amod	homozygous,CbCln3Δex7
R1226	T2109	T2103	conj	CbCln3Δex7,type
R1227	T2110	T2109	punct	/,CbCln3Δex7
R1228	T2111	T2109	nummod	8,CbCln3Δex7
R1229	T2112	T2113	nmod	autofluorescence,formation
R123	T306	T305	punct	", ",heterozygous
R1230	T2113	T2097	dobj	formation,displayed
R1231	T2114	T2112	cc	or,autofluorescence
R1232	T2115	T2116	compound	subunit,c
R1233	T2116	T2117	compound	c,inclusion
R1234	T2117	T2112	conj	inclusion,autofluorescence
R1235	T2118	T2119	punct	(,shown
R1236	T2119	T2097	parataxis	shown,displayed
R1237	T2120	T2119	nsubj	data,shown
R1238	T2121	T2119	neg	not,shown
R1239	T2122	T2119	punct	),shown
R124	T307	T305	cc	and,heterozygous
R1240	T2123	T2097	punct	.,displayed
R1241	T2125	T2126	advmod	However,elevated
R1242	T2127	T2126	punct	", ",elevated
R1243	T2128	T2129	advmod	when,aged
R1244	T2129	T2126	advcl	aged,elevated
R1245	T2130	T2129	nsubjpass	cells,aged
R1246	T2131	T2129	auxpass	were,aged
R1247	T2132	T2129	prep	at,aged
R1248	T2133	T2132	pobj	confluency,at
R1249	T2134	T2133	punct	(,confluency
R125	T308	T305	conj	homozygous,heterozygous
R1250	T2135	T2136	nummod	3,days
R1251	T2136	T2138	npadvmod	days,post-confluency
R1252	T2137	T2135	punct	+,3
R1253	T2138	T2133	advmod	post-confluency,confluency
R1254	T2139	T2126	punct	),elevated
R1255	T2140	T2126	punct	", ",elevated
R1256	T2141	T2142	amod	homozygous,levels
R1257	T2142	T2126	nsubjpass	levels,elevated
R1258	T2143	T2142	nmod	CbCln3Δex7,levels
R1259	T2144	T2143	punct	/,CbCln3Δex7
R126	T309	T310	amod	neuronal,precursor
R1260	T2145	T2143	nummod	8,CbCln3Δex7
R1261	T2146	T2142	amod	cellular,levels
R1262	T2147	T2148	compound	subunit,c
R1263	T2148	T2142	compound	c,levels
R1264	T2149	T2126	auxpass	were,elevated
R1265	T2150	T2126	prep	beyond,elevated
R1266	T2151	T2152	amod	normal,levels
R1267	T2152	T2150	pobj	levels,beyond
R1268	T2153	T2154	amod	wild,type
R1269	T2154	T2152	compound	type,levels
R127	T310	T299	compound	precursor,lines
R1270	T2155	T2154	punct	-,type
R1271	T2156	T2126	prep	by,elevated
R1272	T2157	T2156	pobj	immunostaining,by
R1273	T2158	T2159	punct	(,4a
R1274	T2159	T2157	parataxis	4a,immunostaining
R1275	T2160	T2159	compound	Fig.,4a
R1276	T2161	T2159	punct	),4a
R1277	T2162	T2157	cc	and,immunostaining
R1278	T2163	T2164	compound	immunoblot,analysis
R1279	T2164	T2157	conj	analysis,immunostaining
R128	T311	T299	compound	cell,lines
R1280	T2165	T2166	punct	(,4b
R1281	T2166	T2164	parataxis	4b,analysis
R1282	T2167	T2166	compound	Fig.,4b
R1283	T2168	T2166	punct	),4b
R1284	T2169	T2126	punct	.,elevated
R1285	T2171	T2172	amod	Autofluorescent,signal
R1286	T2172	T2173	nsubj	signal,overlapped
R1287	T2174	T2173	advmod	sometimes,overlapped
R1288	T2175	T2173	prep	with,overlapped
R1289	T2176	T2177	compound	subunit,c
R129	T312	T299	punct	", ",lines
R1290	T2177	T2178	compound	c,signal
R1291	T2178	T2175	pobj	signal,with
R1292	T2179	T2173	punct	", ",overlapped
R1293	T2180	T2173	cc	but,overlapped
R1294	T2181	T2180	advmod	also,but
R1295	T2182	T2173	conj	was,overlapped
R1296	T2183	T2182	acomp	elevated,was
R1297	T2184	T2185	advmod	more,diffusely
R1298	T2185	T2182	advmod	diffusely,was
R1299	T2186	T2187	punct	(,4a
R13	T182	T180	pobj	model,in
R130	T313	T314	dep	which,differentiated
R1300	T2187	T2182	parataxis	4a,was
R1301	T2188	T2187	compound	Fig.,4a
R1302	T2189	T2187	punct	),4a
R1303	T2190	T2173	punct	.,overlapped
R1304	T2192	T2193	advmod	Moreover,displayed
R1305	T2194	T2193	punct	", ",displayed
R1306	T2195	T2196	mark	although,detected
R1307	T2196	T2193	advcl	detected,displayed
R1308	T2197	T2198	amod	multilamellar,profiles
R1309	T2198	T2196	nsubjpass	profiles,detected
R131	T314	T299	relcl	differentiated,lines
R1310	T2199	T2198	punct	"""",profiles
R1311	T2200	T2198	nmod	fingerprint,profiles
R1312	T2201	T2198	punct	"""",profiles
R1313	T2202	T2196	auxpass	were,detected
R1314	T2203	T2196	neg	not,detected
R1315	T2204	T2193	punct	", ",displayed
R1316	T2205	T2206	amod	confluency,aged
R1317	T2206	T2208	amod	aged,cells
R1318	T2207	T2206	punct	-,aged
R1319	T2208	T2193	nsubj	cells,displayed
R132	T315	T314	aux	can,differentiated
R1320	T2209	T2208	amod	homozygous,cells
R1321	T2210	T2208	nmod	CbCln3Δex7,cells
R1322	T2211	T2210	punct	/,CbCln3Δex7
R1323	T2212	T2210	nummod	8,CbCln3Δex7
R1324	T2213	T2214	amod	numerous,abnormalities
R1325	T2214	T2193	dobj	abnormalities,displayed
R1326	T2215	T2214	amod	ultrastructural,abnormalities
R1327	T2216	T2214	prep	including,abnormalities
R1328	T2217	T2218	npadvmod	electron,dense
R1329	T2218	T2219	amod	dense,inclusions
R133	T316	T314	auxpass	be,differentiated
R1330	T2219	T2216	pobj	inclusions,including
R1331	T2220	T2219	amod	characteristic,inclusions
R1332	T2221	T2220	prep	of,characteristic
R1333	T2222	T2221	pobj	lipofuscin,of
R1334	T2223	T2219	cc	and,inclusions
R1335	T2224	T2225	amod	large,autophagosomes
R1336	T2225	T2219	conj	autophagosomes,inclusions
R1337	T2226	T2227	dep	that,contained
R1338	T2227	T2225	relcl	contained,autophagosomes
R1339	T2228	T2229	amod	dense,structures
R134	T317	T314	prep	into,differentiated
R1340	T2229	T2227	dobj	structures,contained
R1341	T2230	T2229	compound	core,structures
R1342	T2231	T2229	punct	", ",structures
R1343	T2232	T2233	amod	degenerating,mitochondria
R1344	T2233	T2229	conj	mitochondria,structures
R1345	T2234	T2233	punct	", ",mitochondria
R1346	T2235	T2233	cc	and,mitochondria
R1347	T2236	T2237	amod	many,vesicles
R1348	T2237	T2233	conj	vesicles,mitochondria
R1349	T2238	T2237	amod	smaller,vesicles
R135	T318	T319	npadvmod	MAP,positive
R1350	T2239	T2240	punct	(,4c
R1351	T2240	T2227	parataxis	4c,contained
R1352	T2241	T2240	compound	Fig.,4c
R1353	T2242	T2240	punct	),4c
R1354	T2243	T2193	punct	.,displayed
R1355	T2245	T2246	compound	Inclusion,bodies
R1356	T2246	T2247	nsubjpass	bodies,observed
R1357	T2248	T2246	cc	and,bodies
R1358	T2249	T2246	conj	autophagosomes,bodies
R1359	T2250	T2247	auxpass	were,observed
R136	T319	T325	amod	positive,cells
R1360	T2251	T2247	advmod	infrequently,observed
R1361	T2252	T2247	prep	in,observed
R1362	T2253	T2254	npadvmod	confluency,aged
R1363	T2254	T2256	amod	aged,cultures
R1364	T2255	T2254	punct	-,aged
R1365	T2256	T2252	pobj	cultures,in
R1366	T2257	T2258	amod	wild,type
R1367	T2258	T2256	compound	type,cultures
R1368	T2259	T2258	punct	-,type
R1369	T2260	T2261	punct	(,shown
R137	T320	T318	punct	-,MAP
R1370	T2261	T2247	parataxis	shown,observed
R1371	T2262	T2261	nsubj	data,shown
R1372	T2263	T2261	neg	not,shown
R1373	T2264	T2261	punct	),shown
R1374	T2265	T2247	punct	.,observed
R1375	T2526	T2527	amod	Homozygous,cells
R1376	T2527	T2531	nsubj	cells,process
R1377	T2528	T2527	nmod	CbCln3Δex7,cells
R1378	T2529	T2528	punct	/,CbCln3Δex7
R1379	T2530	T2528	nummod	8,CbCln3Δex7
R138	T321	T318	nummod	2,MAP
R1380	T2532	T2527	cc	and,cells
R1381	T2533	T2534	nmod	Cln3Δex7,mice
R1382	T2534	T2527	conj	mice,cells
R1383	T2535	T2533	punct	/,Cln3Δex7
R1384	T2536	T2533	nummod	8,Cln3Δex7
R1385	T2537	T2534	amod	knock,mice
R1386	T2538	T2537	punct	-,knock
R1387	T2539	T2537	prt	in,knock
R1388	T2540	T2541	compound	cathepsin,D
R1389	T2541	T2531	dobj	D,process
R139	T322	T318	cc	and,MAP
R1390	T2542	T2531	advmod	abnormally,process
R1391	T2544	T2545	nsubj	We,investigated
R1392	T2546	T2545	advmod	next,investigated
R1393	T2547	T2548	det	the,basis
R1394	T2548	T2545	dobj	basis,investigated
R1395	T2549	T2548	prep	for,basis
R1396	T2550	T2551	compound	subunit,c
R1397	T2551	T2552	compound	c,accumulation
R1398	T2552	T2549	pobj	accumulation,for
R1399	T2553	T2545	punct	", ",investigated
R14	T183	T184	amod	cerebellar,cell
R140	T323	T318	conj	NeuN,MAP
R1400	T2554	T2545	advcl	testing,investigated
R1401	T2555	T2556	det	the,hypothesis
R1402	T2556	T2554	dobj	hypothesis,testing
R1403	T2557	T2558	mark	that,is
R1404	T2558	T2556	acl	is,hypothesis
R1405	T2559	T2560	compound	cathepsin,D
R1406	T2560	T2558	nsubj	D,is
R1407	T2561	T2558	acomp	abnormal,is
R1408	T2562	T2563	mark	since,is
R1409	T2563	T2558	advcl	is,is
R141	T324	T319	punct	-,positive
R1410	T2564	T2563	nsubj	it,is
R1411	T2565	T2563	acomp	required,is
R1412	T2566	T2565	prep	for,required
R1413	T2567	T2568	compound	ATP,degradation
R1414	T2568	T2566	pobj	degradation,for
R1415	T2569	T2568	compound	synthase,degradation
R1416	T2570	T2571	compound	subunit,c
R1417	T2571	T2568	compound	c,degradation
R1418	T2572	T2568	prep	in,degradation
R1419	T2573	T2574	det	the,lysosome
R142	T325	T317	pobj	cells,into
R1420	T2574	T2572	pobj	lysosome,in
R1421	T2575	T2576	punct	[,13
R1422	T2576	T2554	parataxis	13,testing
R1423	T2577	T2576	punct	],13
R1424	T2578	T2545	punct	.,investigated
R1425	T2580	T2581	nsubj	We,tested
R1426	T2582	T2581	advmod	first,tested
R1427	T2583	T2584	compound	cathepsin,D
R1428	T2584	T2581	dobj	D,tested
R1429	T2585	T2584	appos	transport,D
R143	T326	T325	punct	", ",cells
R1430	T2586	T2585	cc	and,transport
R1431	T2587	T2585	conj	processing,transport
R1432	T2588	T2581	prep	in,tested
R1433	T2589	T2590	amod	homozygous,cells
R1434	T2590	T2588	pobj	cells,in
R1435	T2591	T2590	nmod	CbCln3Δex7,cells
R1436	T2592	T2591	punct	/,CbCln3Δex7
R1437	T2593	T2591	nummod	8,CbCln3Δex7
R1438	T2594	T2590	cc	and,cells
R1439	T2595	T2596	nmod	Cln3Δex7,mice
R144	T327	T328	npadvmod	neuron,like
R1440	T2596	T2590	conj	mice,cells
R1441	T2597	T2595	punct	/,Cln3Δex7
R1442	T2598	T2595	nummod	8,Cln3Δex7
R1443	T2599	T2581	advcl	using,tested
R1444	T2600	T2601	amod	anti-cathepsin,D
R1445	T2601	T2602	amod	D,antibody
R1446	T2602	T2599	dobj	antibody,using
R1447	T2603	T2604	dep	that,recognizes
R1448	T2604	T2602	relcl	recognizes,antibody
R1449	T2605	T2606	amod	unprocessed,isoforms
R145	T328	T325	amod	like,cells
R1450	T2606	T2604	dobj	isoforms,recognizes
R1451	T2607	T2605	cc	and,unprocessed
R1452	T2608	T2605	conj	processed,unprocessed
R1453	T2609	T2610	compound	cathepsin,D
R1454	T2610	T2606	compound	D,isoforms
R1455	T2611	T2581	punct	.,tested
R1456	T2613	T2614	nsubj	Immunostaining,revealed
R1457	T2615	T2613	prep	of,Immunostaining
R1458	T2616	T2617	amod	wild,type
R1459	T2617	T2619	nmod	type,cells
R146	T329	T328	punct	-,like
R1460	T2618	T2617	punct	-,type
R1461	T2619	T2615	pobj	cells,of
R1462	T2620	T2617	cc	and,type
R1463	T2621	T2617	conj	homozygous,type
R1464	T2622	T2619	nmod	CbCln3Δex7,cells
R1465	T2623	T2622	punct	/,CbCln3Δex7
R1466	T2624	T2622	nummod	8,CbCln3Δex7
R1467	T2625	T2626	det	a,distribution
R1468	T2626	T2614	dobj	distribution,revealed
R1469	T2627	T2626	amod	perinuclear,distribution
R147	T330	T276	punct	.,used
R1470	T2628	T2627	cc	and,perinuclear
R1471	T2629	T2627	conj	punctate,perinuclear
R1472	T2630	T2626	amod	vesicular,distribution
R1473	T2631	T2632	compound	cathepsin,D
R1474	T2632	T2626	compound	D,distribution
R1475	T2633	T2614	punct	", ",revealed
R1476	T2634	T2614	advcl	consistent,revealed
R1477	T2635	T2634	prep	with,consistent
R1478	T2636	T2637	poss	its,transport
R1479	T2637	T2635	pobj	transport,with
R148	T332	T333	amod	Homozygous,cells
R1480	T2638	T2637	cc	and,transport
R1481	T2639	T2637	conj	processing,transport
R1482	T2640	T2637	prep	through,transport
R1483	T2641	T2642	det	the,pathway
R1484	T2642	T2640	pobj	pathway,through
R1485	T2643	T2642	amod	secretory,pathway
R1486	T2644	T2637	cc	and,transport
R1487	T2645	T2637	conj	delivery,transport
R1488	T2646	T2645	prep	to,delivery
R1489	T2647	T2648	det	the,lysosome
R149	T333	T338	nsubj	cells,express
R1490	T2648	T2646	pobj	lysosome,to
R1491	T2649	T2650	punct	(,5a
R1492	T2650	T2614	parataxis	5a,revealed
R1493	T2651	T2650	compound	Fig.,5a
R1494	T2652	T2650	punct	),5a
R1495	T2653	T2614	punct	.,revealed
R1496	T2655	T2656	advmod	However,was
R1497	T2657	T2656	punct	", ",was
R1498	T2658	T2656	prep	in,was
R1499	T2659	T2660	amod	homozygous,cells
R15	T184	T182	compound	cell,model
R150	T334	T333	nmod	CbCln3Δex7,cells
R1500	T2660	T2658	pobj	cells,in
R1501	T2661	T2660	nmod	CbCln3Δex7,cells
R1502	T2662	T2661	punct	/,CbCln3Δex7
R1503	T2663	T2661	nummod	8,CbCln3Δex7
R1504	T2664	T2656	punct	", ",was
R1505	T2665	T2666	compound	cathepsin,D
R1506	T2666	T2667	compound	D,distribution
R1507	T2667	T2656	nsubj	distribution,was
R1508	T2668	T2669	advmod	less,vesicular
R1509	T2669	T2656	acomp	vesicular,was
R151	T335	T334	punct	/,CbCln3Δex7
R1510	T2670	T2669	cc	and,vesicular
R1511	T2671	T2672	advmod	more,clustered
R1512	T2672	T2669	conj	clustered,vesicular
R1513	T2673	T2672	amod	perinuclear,clustered
R1514	T2674	T2672	punct	-,clustered
R1515	T2675	T2669	prep	than,vesicular
R1516	T2676	T2675	prep	in,than
R1517	T2677	T2678	amod	wild,type
R1518	T2678	T2680	compound	type,cells
R1519	T2679	T2678	punct	-,type
R152	T336	T334	nummod	8,CbCln3Δex7
R1520	T2680	T2676	pobj	cells,in
R1521	T2681	T2656	punct	.,was
R1522	T2683	T2684	nsubj	Immunoblots,showed
R1523	T2685	T2683	prep	of,Immunoblots
R1524	T2686	T2687	amod	homozygous,extracts
R1525	T2687	T2685	pobj	extracts,of
R1526	T2688	T2689	nmod	CbCln3Δex7,cell
R1527	T2689	T2687	nmod	cell,extracts
R1528	T2690	T2688	punct	/,CbCln3Δex7
R1529	T2691	T2688	nummod	8,CbCln3Δex7
R153	T337	T333	compound	precursor,cells
R1530	T2692	T2689	cc	and,cell
R1531	T2693	T2694	nmod	Cln3Δex7,tissue
R1532	T2694	T2689	conj	tissue,cell
R1533	T2695	T2693	punct	/,Cln3Δex7
R1534	T2696	T2693	nummod	8,Cln3Δex7
R1535	T2697	T2684	advmod	also,showed
R1536	T2698	T2699	amod	altered,levels
R1537	T2699	T2684	dobj	levels,showed
R1538	T2700	T2699	amod	relative,levels
R1539	T2701	T2699	prep	of,levels
R154	T339	T340	amod	low,levels
R1540	T2702	T2703	compound	cathepsin,D
R1541	T2703	T2704	compound	D,isoforms
R1542	T2704	T2701	pobj	isoforms,of
R1543	T2705	T2706	punct	(,5b
R1544	T2706	T2684	parataxis	5b,showed
R1545	T2707	T2706	compound	Fig.,5b
R1546	T2708	T2706	punct	),5b
R1547	T2709	T2684	punct	.,showed
R1548	T2711	T2712	compound	Cathepsin,D
R1549	T2712	T2713	compound	D,isoforms
R155	T340	T338	dobj	levels,express
R1550	T2713	T2714	nsubj	isoforms,represent
R1551	T2715	T2713	punct	", ",isoforms
R1552	T2716	T2713	acl	identified,isoforms
R1553	T2717	T2716	agent	by,identified
R1554	T2718	T2719	amod	relative,weights
R1555	T2719	T2717	pobj	weights,by
R1556	T2720	T2719	amod	molecular,weights
R1557	T2721	T2714	punct	", ",represent
R1558	T2722	T2723	det	the,precursor
R1559	T2723	T2714	dobj	precursor,represent
R156	T341	T340	prep	of,levels
R1560	T2724	T2725	punct	~,45
R1561	T2725	T2726	nummod	45,kDa
R1562	T2726	T2723	compound	kDa,precursor
R1563	T2727	T2723	punct	", ",precursor
R1564	T2728	T2729	det	the,form
R1565	T2729	T2723	conj	form,precursor
R1566	T2730	T2731	punct	~,43
R1567	T2731	T2732	nummod	43,kDa
R1568	T2732	T2729	nmod	kDa,form
R1569	T2733	T2729	amod	intermediate,form
R157	T342	T343	compound	mutant,battenin
R1570	T2734	T2735	amod	single,chain
R1571	T2735	T2729	compound	chain,form
R1572	T2736	T2729	prep	of,form
R1573	T2737	T2738	det	the,enzyme
R1574	T2738	T2736	pobj	enzyme,of
R1575	T2739	T2729	punct	", ",form
R1576	T2740	T2729	cc	and,form
R1577	T2741	T2742	det	the,chain
R1578	T2742	T2729	conj	chain,form
R1579	T2743	T2744	nummod	31,kDa
R158	T343	T341	pobj	battenin,of
R1580	T2744	T2742	nmod	kDa,chain
R1581	T2745	T2742	amod	heavy,chain
R1582	T2746	T2742	prep	of,chain
R1583	T2747	T2748	det	the,form
R1584	T2748	T2746	pobj	form,of
R1585	T2749	T2750	amod	double,chain
R1586	T2750	T2748	compound	chain,form
R1587	T2751	T2750	punct	-,chain
R1588	T2752	T2748	prep	of,form
R1589	T2753	T2754	det	the,enzyme
R159	T344	T338	cc	and,express
R1590	T2754	T2752	pobj	enzyme,of
R1591	T2755	T2754	amod	mature,enzyme
R1592	T2756	T2757	punct	[,14
R1593	T2757	T2714	parataxis	14,represent
R1594	T2758	T2757	punct	],14
R1595	T2759	T2714	punct	.,represent
R1596	T2761	T2762	prep	In,reduced
R1597	T2763	T2764	amod	homozygous,extracts
R1598	T2764	T2761	pobj	extracts,In
R1599	T2765	T2766	nmod	CbCln3Δex7,cell
R16	T185	T182	prep	of,model
R160	T345	T338	punct	", ",express
R1600	T2766	T2764	nmod	cell,extracts
R1601	T2767	T2765	punct	/,CbCln3Δex7
R1602	T2768	T2765	nummod	8,CbCln3Δex7
R1603	T2769	T2766	cc	and,cell
R1604	T2770	T2771	nmod	Cln3Δex7,tissue
R1605	T2771	T2766	conj	tissue,cell
R1606	T2772	T2770	punct	/,Cln3Δex7
R1607	T2773	T2770	nummod	8,Cln3Δex7
R1608	T2774	T2762	punct	", ",reduced
R1609	T2775	T2776	det	the,precursor
R161	T346	T347	advmod	when,aged
R1610	T2776	T2762	nsubjpass	precursor,reduced
R1611	T2777	T2776	cc	and,precursor
R1612	T2778	T2779	amod	heavy,chains
R1613	T2779	T2776	conj	chains,precursor
R1614	T2780	T2762	auxpass	were,reduced
R1615	T2781	T2762	punct	", ",reduced
R1616	T2782	T2762	cc	and,reduced
R1617	T2783	T2784	det	the,chain
R1618	T2784	T2786	nsubj	chain,was
R1619	T2785	T2784	amod	single,chain
R162	T347	T348	advcl	aged,accumulate
R1620	T2786	T2762	conj	was,reduced
R1621	T2787	T2788	advmod	slightly,elevated
R1622	T2788	T2786	acomp	elevated,was
R1623	T2789	T2786	prep	compared,was
R1624	T2790	T2789	prep	to,compared
R1625	T2791	T2792	amod	wild,type
R1626	T2792	T2794	compound	type,extracts
R1627	T2793	T2792	punct	-,type
R1628	T2794	T2790	pobj	extracts,to
R1629	T2795	T2786	punct	.,was
R163	T348	T338	conj	accumulate,express
R1630	T2797	T2798	det	The,media
R1631	T2798	T2801	nsubj	media,contain
R1632	T2799	T2800	amod	cellular,growth
R1633	T2800	T2798	compound	growth,media
R1634	T2802	T2801	aux	did,contain
R1635	T2803	T2801	neg	not,contain
R1636	T2804	T2805	amod	altered,levels
R1637	T2805	T2801	dobj	levels,contain
R1638	T2806	T2805	prep	of,levels
R1639	T2807	T2808	compound	cathepsin,D
R164	T349	T347	prep	at,aged
R1640	T2808	T2806	pobj	D,of
R1641	T2809	T2801	punct	", ",contain
R1642	T2810	T2801	advcl	indicating,contain
R1643	T2811	T2812	compound	enzyme,secretion
R1644	T2812	T2813	nsubjpass	secretion,affected
R1645	T2813	T2810	advcl	affected,indicating
R1646	T2814	T2813	auxpass	was,affected
R1647	T2815	T2813	neg	not,affected
R1648	T2816	T2801	punct	.,contain
R1649	T2818	T2819	amod	Heterozygous,mice
R165	T350	T349	pobj	confluency,at
R1650	T2819	T2823	nsubj	mice,were
R1651	T2820	T2819	nmod	Cln3Δex7,mice
R1652	T2821	T2820	punct	/,Cln3Δex7
R1653	T2822	T2820	nummod	8,Cln3Δex7
R1654	T2824	T2819	cc	and,mice
R1655	T2825	T2826	nmod	CbCln3Δex7,cells
R1656	T2826	T2819	conj	cells,mice
R1657	T2827	T2825	punct	/,CbCln3Δex7
R1658	T2828	T2825	nummod	8,CbCln3Δex7
R1659	T2829	T2823	acomp	indistinguishable,were
R166	T351	T348	punct	", ",accumulate
R1660	T2830	T2829	prep	from,indistinguishable
R1661	T2831	T2832	amod	wild,type
R1662	T2832	T2830	pobj	type,from
R1663	T2833	T2832	punct	-,type
R1664	T2834	T2823	punct	", ",were
R1665	T2835	T2836	mark	as,expected
R1666	T2836	T2823	advcl	expected,were
R1667	T2837	T2836	prep	for,expected
R1668	T2838	T2839	det	a,phenotype
R1669	T2839	T2837	pobj	phenotype,for
R167	T352	T353	compound	ATPase,c
R1670	T2840	T2839	amod	recessive,phenotype
R1671	T2841	T2839	compound	disease,phenotype
R1672	T2842	T2843	punct	(,shown
R1673	T2843	T2836	parataxis	shown,expected
R1674	T2844	T2843	nsubj	data,shown
R1675	T2845	T2843	neg	not,shown
R1676	T2846	T2843	punct	),shown
R1677	T2847	T2823	punct	.,were
R1678	T2855	T2856	amod	c,t
R1679	T2862	T2863	amod	e,a
R168	T353	T348	dobj	c,accumulate
R1680	T2865	T2866	advmod	l,ed
R1681	T2867	T2866	nsubj	er,ed
R1682	T2868	T2867	nmod	t,er
R1683	T2873	T2874	nsubjpass	p,tested
R1684	T2876	T2877	compound	sin,D
R1685	T2877	T2875	compound	D,processing
R1686	T2878	T2873	prep	on,p
R1687	T2879	T2880	amod	enzymatic,activity
R1688	T2880	T2878	pobj	activity,on
R1689	T2881	T2874	auxpass	was,tested
R169	T354	T353	compound	subunit,c
R1690	T2882	T2874	advmod	next,tested
R1691	T2883	T2884	aux	to,determine
R1692	T2884	T2874	advcl	determine,tested
R1693	T2885	T2886	mark	if,accounts
R1694	T2886	T2884	ccomp	accounts,determine
R1695	T2887	T2888	amod	altered,activity
R1696	T2888	T2886	nsubj	activity,accounts
R1697	T2889	T2888	amod	enzymatic,activity
R1698	T2890	T2886	prep	for,accounts
R1699	T2891	T2892	amod	inefficient,turnover
R17	T186	T187	amod	juvenile,lipofuscinosis
R170	T355	T338	punct	.,express
R1700	T2892	T2890	pobj	turnover,for
R1701	T2893	T2894	compound	subunit,c
R1702	T2894	T2892	compound	c,turnover
R1703	T2895	T2874	punct	.,tested
R1704	T2897	T2898	prep	In,altered
R1705	T2899	T2900	det	a,assay
R1706	T2900	T2897	pobj	assay,In
R1707	T2901	T2900	amod	fluorogenic,assay
R1708	T2902	T2903	advmod	in,vitro
R1709	T2903	T2900	amod	vitro,assay
R171	T357	T358	amod	Recessive,phenotypes
R1710	T2904	T2898	punct	", ",altered
R1711	T2905	T2906	compound	cathepsin,D
R1712	T2906	T2907	compound	D,activity
R1713	T2907	T2898	nsubjpass	activity,altered
R1714	T2908	T2907	prep	in,activity
R1715	T2909	T2910	amod	total,extracts
R1716	T2910	T2908	pobj	extracts,in
R1717	T2911	T2910	amod	cellular,extracts
R1718	T2912	T2898	auxpass	was,altered
R1719	T2913	T2898	neg	not,altered
R172	T358	T359	nsubjpass	phenotypes,observed
R1720	T2914	T2898	advmod	significantly,altered
R1721	T2915	T2898	prep	in,altered
R1722	T2916	T2917	amod	homozygous,cells
R1723	T2917	T2915	pobj	cells,in
R1724	T2918	T2917	nmod	CbCln3Δex7,cells
R1725	T2919	T2918	punct	/,CbCln3Δex7
R1726	T2920	T2918	nummod	8,CbCln3Δex7
R1727	T2921	T2922	punct	(,RFU
R1728	T2922	T2917	parataxis	RFU,cells
R1729	T2923	T2924	quantmod	376,89
R173	T360	T359	auxpass	are,observed
R1730	T2924	T2922	nummod	89,RFU
R1731	T2925	T2924	punct	±,89
R1732	T2926	T2927	punct	/,protein
R1733	T2927	T2922	prep	protein,RFU
R1734	T2928	T2927	nmod	μg,protein
R1735	T2929	T2927	amod	total,protein
R1736	T2930	T2922	punct	),RFU
R1737	T2931	T2917	punct	", ",cells
R1738	T2932	T2917	cc	versus,cells
R1739	T2933	T2934	amod	wild,type
R174	T361	T359	advmod	also,observed
R1740	T2934	T2936	compound	type,cells
R1741	T2935	T2934	punct	-,type
R1742	T2936	T2917	conj	cells,cells
R1743	T2937	T2938	punct	(,RFU
R1744	T2938	T2936	parataxis	RFU,cells
R1745	T2939	T2940	quantmod	324,58
R1746	T2940	T2938	nummod	58,RFU
R1747	T2941	T2940	quantmod	±,58
R1748	T2942	T2943	punct	/,protein
R1749	T2943	T2938	prep	protein,RFU
R175	T362	T359	prep	at,observed
R1750	T2944	T2943	nmod	μg,protein
R1751	T2945	T2943	amod	total,protein
R1752	T2946	T2938	punct	),RFU
R1753	T2947	T2898	punct	", ",altered
R1754	T2948	T2949	mark	although,observed
R1755	T2949	T2898	advcl	observed,altered
R1756	T2950	T2951	det	a,trend
R1757	T2951	T2949	nsubjpass	trend,observed
R1758	T2952	T2951	amod	consistent,trend
R1759	T2953	T2951	prep	towards,trend
R176	T363	T364	amod	sub-confluent,growth
R1760	T2954	T2955	amod	increased,activity
R1761	T2955	T2953	pobj	activity,towards
R1762	T2956	T2955	amod	enzymatic,activity
R1763	T2957	T2955	prep	in,activity
R1764	T2958	T2959	compound	mutant,cells
R1765	T2959	T2957	pobj	cells,in
R1766	T2960	T2949	auxpass	was,observed
R1767	T2961	T2898	punct	.,altered
R1768	T2963	T2964	advmod	Thus,disrupted
R1769	T2965	T2964	punct	", ",disrupted
R177	T364	T362	pobj	growth,at
R1770	T2966	T2967	compound	cathepsin,D
R1771	T2967	T2964	nsubjpass	D,disrupted
R1772	T2968	T2967	appos	transport,D
R1773	T2969	T2968	cc	and,transport
R1774	T2970	T2968	conj	processing,transport
R1775	T2971	T2964	auxpass	are,disrupted
R1776	T2972	T2964	prep	in,disrupted
R1777	T2973	T2974	amod	homozygous,cells
R1778	T2974	T2972	pobj	cells,in
R1779	T2975	T2974	nmod	CbCln3Δex7,cells
R178	T365	T359	punct	;,observed
R1780	T2976	T2975	punct	/,CbCln3Δex7
R1781	T2977	T2975	nummod	8,CbCln3Δex7
R1782	T2978	T2964	prep	in,disrupted
R1783	T2979	T2980	det	a,manner
R1784	T2980	T2978	pobj	manner,in
R1785	T2981	T2980	amod	such,manner
R1786	T2982	T2983	mark	that,appears
R1787	T2983	T2981	ccomp	appears,such
R1788	T2984	T2985	amod	enzymatic,activity
R1789	T2985	T2983	nsubj	activity,appears
R179	T366	T367	compound	cathepsin,D
R1790	T2986	T2987	aux	to,be
R1791	T2987	T2983	xcomp	be,appears
R1792	T2988	T2989	advmod	relatively,unaffected
R1793	T2989	T2987	acomp	unaffected,be
R1794	T2990	T2964	punct	.,disrupted
R1795	T3349	T3350	amod	Homozygous,cells
R1796	T3350	T3354	nsubj	cells,show
R1797	T3351	T3350	nmod	CbCln3Δex7,cells
R1798	T3352	T3351	punct	/,CbCln3Δex7
R1799	T3353	T3351	nummod	8,CbCln3Δex7
R18	T188	T187	amod	neuronal,lipofuscinosis
R180	T367	T368	nsubjpass	D,altered
R1800	T3355	T3356	amod	abnormal,organelles
R1801	T3356	T3354	dobj	organelles,show
R1802	T3357	T3356	compound	membrane,organelles
R1803	T3359	T3360	det	The,transport
R1804	T3360	T3362	nsubj	transport,suggested
R1805	T3361	T3360	amod	abnormal,transport
R1806	T3362	T3368	ccomp	suggested,surveyed
R1807	T3363	T3360	cc	and,transport
R1808	T3364	T3360	conj	processing,transport
R1809	T3365	T3360	prep	of,transport
R181	T368	T359	conj	altered,observed
R1810	T3366	T3367	compound	cathepsin,D
R1811	T3367	T3365	pobj	D,of
R1812	T3369	T3370	compound	membrane,trafficking
R1813	T3370	T3371	compound	trafficking,disruptions
R1814	T3371	T3362	dobj	disruptions,suggested
R1815	T3372	T3362	prep	in,suggested
R1816	T3373	T3374	amod	homozygous,cells
R1817	T3374	T3372	pobj	cells,in
R1818	T3375	T3374	nmod	CbCln3Δex7,cells
R1819	T3376	T3375	punct	/,CbCln3Δex7
R182	T369	T367	appos	transport,D
R1820	T3377	T3375	nummod	8,CbCln3Δex7
R1821	T3378	T3368	punct	;,surveyed
R1822	T3379	T3368	advmod	therefore,surveyed
R1823	T3380	T3368	punct	", ",surveyed
R1824	T3381	T3368	nsubj	we,surveyed
R1825	T3382	T3383	det	the,distribution
R1826	T3383	T3368	dobj	distribution,surveyed
R1827	T3384	T3383	amod	subcellular,distribution
R1828	T3385	T3383	cc	and,distribution
R1829	T3386	T3383	conj	morphology,distribution
R183	T370	T369	cc	and,transport
R1830	T3387	T3383	prep	of,distribution
R1831	T3388	T3389	compound	membrane,organelles
R1832	T3389	T3387	pobj	organelles,of
R1833	T3390	T3368	punct	.,surveyed
R1834	T3392	T3393	nsubj	Components,appear
R1835	T3394	T3392	prep	of,Components
R1836	T3395	T3396	det	the,pathway
R1837	T3396	T3394	pobj	pathway,of
R1838	T3397	T3396	amod	secretory,pathway
R1839	T3398	T3392	punct	", ",Components
R184	T371	T369	conj	processing,transport
R1840	T3399	T3392	prep	including,Components
R1841	T3400	T3401	det	the,ER
R1842	T3401	T3399	pobj	ER,including
R1843	T3402	T3401	punct	", ",ER
R1844	T3403	T3404	compound	cis,Golgi
R1845	T3404	T3401	conj	Golgi,ER
R1846	T3405	T3404	punct	-,Golgi
R1847	T3406	T3404	punct	", ",Golgi
R1848	T3407	T3404	cc	and,Golgi
R1849	T3408	T3409	compound	trans,Golgi
R185	T372	T368	auxpass	are,altered
R1850	T3409	T3404	conj	Golgi,Golgi
R1851	T3410	T3409	punct	-,Golgi
R1852	T3411	T3393	punct	", ",appear
R1853	T3412	T3393	aux	did,appear
R1854	T3413	T3393	neg	not,appear
R1855	T3414	T3393	oprd	altered,appear
R1856	T3415	T3414	prep	from,altered
R1857	T3416	T3417	amod	wild,type
R1858	T3417	T3419	compound	type,appearance
R1859	T3418	T3417	punct	-,type
R186	T373	T368	punct	", ",altered
R1860	T3419	T3415	pobj	appearance,from
R1861	T3420	T3421	advmod	when,labeled
R1862	T3421	T3393	advcl	labeled,appear
R1863	T3422	T3421	prep	with,labeled
R1864	T3423	T3424	det	the,markers
R1865	T3424	T3422	pobj	markers,with
R1866	T3425	T3424	amod	respective,markers
R1867	T3426	T3424	punct	", ",markers
R1868	T3427	T3428	compound	protein,isomerase
R1869	T3428	T3424	appos	isomerase,markers
R187	T374	T375	mark	although,affected
R1870	T3429	T3428	compound	disulfide,isomerase
R1871	T3430	T3428	punct	(,isomerase
R1872	T3431	T3428	appos	PDI,isomerase
R1873	T3432	T3428	punct	),isomerase
R1874	T3433	T3428	punct	", ",isomerase
R1875	T3434	T3428	conj	GM130,isomerase
R1876	T3435	T3434	punct	", ",GM130
R1877	T3436	T3434	cc	and,GM130
R1878	T3437	T3434	conj	VVL,GM130
R1879	T3438	T3439	punct	(,shown
R188	T375	T381	advcl	affected,altered
R1880	T3439	T3393	parataxis	shown,appear
R1881	T3440	T3439	nsubj	data,shown
R1882	T3441	T3439	neg	not,shown
R1883	T3442	T3439	punct	),shown
R1884	T3443	T3393	punct	.,appear
R1885	T3445	T3446	prep	By,had
R1886	T3447	T3445	pobj	contrast,By
R1887	T3448	T3446	punct	", ",had
R1888	T3449	T3450	det	the,markers
R1889	T3450	T3446	nsubj	markers,had
R189	T376	T377	compound	enzyme,activity
R1890	T3451	T3450	amod	lysosomal,markers
R1891	T3452	T3450	punct	", ",markers
R1892	T3453	T3450	appos	Lysotracker,markers
R1893	T3454	T3453	cc	and,Lysotracker
R1894	T3455	T3453	conj	Lamp,Lysotracker
R1895	T3456	T3455	nummod	2,Lamp
R1896	T3457	T3458	advmod	significantly,altered
R1897	T3458	T3459	amod	altered,signal
R1898	T3459	T3446	dobj	signal,had
R1899	T3460	T3446	prep	in,had
R19	T189	T187	compound	ceroid,lipofuscinosis
R190	T377	T375	nsubjpass	activity,affected
R1900	T3461	T3462	amod	homozygous,cells
R1901	T3462	T3460	pobj	cells,in
R1902	T3463	T3462	nmod	CbCln3Δex7,cells
R1903	T3464	T3463	punct	/,CbCln3Δex7
R1904	T3465	T3463	nummod	8,CbCln3Δex7
R1905	T3466	T3462	punct	", ",cells
R1906	T3467	T3462	cc	versus,cells
R1907	T3468	T3469	amod	wild,type
R1908	T3469	T3471	compound	type,cells
R1909	T3470	T3469	punct	-,type
R191	T378	T375	auxpass	is,affected
R1910	T3471	T3462	conj	cells,cells
R1911	T3472	T3446	punct	.,had
R1912	T3474	T3475	amod	Wild,type
R1913	T3475	T3477	compound	type,cells
R1914	T3476	T3475	punct	-,type
R1915	T3477	T3478	nsubj	cells,exhibited
R1916	T3479	T3480	advmod	brightly,stained
R1917	T3480	T3481	amod	stained,lysosomes
R1918	T3481	T3478	dobj	lysosomes,exhibited
R1919	T3482	T3483	dep	that,were
R192	T379	T375	neg	not,affected
R1920	T3483	T3481	relcl	were,lysosomes
R1921	T3484	T3483	acomp	large,were
R1922	T3485	T3484	cc	and,large
R1923	T3486	T3484	conj	clustered,large
R1924	T3487	T3483	prep	in,were
R1925	T3488	T3489	det	the,region
R1926	T3489	T3487	pobj	region,in
R1927	T3490	T3489	amod	perinuclear,region
R1928	T3491	T3492	mark	whereas,were
R1929	T3492	T3483	advcl	were,were
R193	T380	T375	advmod	significantly,affected
R1930	T3493	T3494	amod	homozygous,lysosomes
R1931	T3494	T3492	nsubj	lysosomes,were
R1932	T3495	T3494	nmod	CbCln3Δex7,lysosomes
R1933	T3496	T3495	punct	/,CbCln3Δex7
R1934	T3497	T3495	nummod	8,CbCln3Δex7
R1935	T3498	T3499	advmod	lightly,stained
R1936	T3499	T3500	amod	stained,vesicles
R1937	T3500	T3492	attr	vesicles,were
R1938	T3501	T3500	punct	", ",vesicles
R1939	T3502	T3500	amod	smaller,vesicles
R194	T381	T368	conj	altered,altered
R1940	T3503	T3504	dep	that,scattered
R1941	T3504	T3500	relcl	scattered,vesicles
R1942	T3505	T3504	auxpass	were,scattered
R1943	T3506	T3507	advmod	more,diffusely
R1944	T3507	T3504	advmod	diffusely,scattered
R1945	T3508	T3504	prep	in,scattered
R1946	T3509	T3510	det	the,cytoplasm
R1947	T3510	T3508	pobj	cytoplasm,in
R1948	T3511	T3510	prep	of,cytoplasm
R1949	T3512	T3513	det	the,cell
R195	T382	T381	punct	", ",altered
R1950	T3513	T3511	pobj	cell,of
R1951	T3514	T3515	punct	(,Fig.
R1952	T3515	T3478	parataxis	Fig.,exhibited
R1953	T3516	T3515	nummod	6,Fig.
R1954	T3517	T3515	punct	),Fig.
R1955	T3518	T3478	punct	.,exhibited
R1956	T3520	T3521	nmod	Lamp,distribution
R1957	T3521	T3523	nsubjpass	distribution,altered
R1958	T3522	T3520	nummod	1,Lamp
R1959	T3524	T3523	auxpass	was,altered
R196	T383	T384	amod	lysosomal,size
R1960	T3525	T3523	advmod	also,altered
R1961	T3526	T3523	punct	", ",altered
R1962	T3527	T3528	mark	as,noted
R1963	T3528	T3523	advcl	noted,altered
R1964	T3529	T3528	advmod	previously,noted
R1965	T3530	T3531	punct	(,Fig.
R1966	T3531	T3523	parataxis	Fig.,altered
R1967	T3532	T3531	nummod	3,Fig.
R1968	T3533	T3531	punct	),Fig.
R1969	T3534	T3523	punct	.,altered
R197	T384	T381	nsubjpass	size,altered
R1970	T3536	T3537	advmod	However,were
R1971	T3538	T3537	punct	", ",were
R1972	T3539	T3540	nmod	Lamp,levels
R1973	T3540	T3537	nsubj	levels,were
R1974	T3541	T3539	nummod	1,Lamp
R1975	T3542	T3539	cc	and,Lamp
R1976	T3543	T3539	conj	Lamp,Lamp
R1977	T3544	T3543	nummod	2,Lamp
R1978	T3545	T3546	amod	total,protein
R1979	T3546	T3540	compound	protein,levels
R198	T385	T384	cc	and,size
R1980	T3547	T3537	acomp	similar,were
R1981	T3548	T3537	prep	in,were
R1982	T3549	T3550	amod	wild,type
R1983	T3550	T3552	nmod	type,cells
R1984	T3551	T3550	punct	-,type
R1985	T3552	T3548	pobj	cells,in
R1986	T3553	T3550	cc	and,type
R1987	T3554	T3550	conj	homozygous,type
R1988	T3555	T3552	nmod	CbCln3Δex7,cells
R1989	T3556	T3555	punct	/,CbCln3Δex7
R199	T386	T384	conj	distribution,size
R1990	T3557	T3555	nummod	8,CbCln3Δex7
R1991	T3558	T3537	prep	by,were
R1992	T3559	T3560	compound	immunoblot,analysis
R1993	T3560	T3558	pobj	analysis,by
R1994	T3561	T3537	punct	", ",were
R1995	T3562	T3537	advcl	indicating,were
R1996	T3563	T3564	det	the,signal
R1997	T3564	T3566	nsubj	signal,reflects
R1998	T3565	T3564	amod	altered,signal
R1999	T3566	T3562	advcl	reflects,indicating
R20	T194	T195	nsubj	JNCL,is
R200	T387	T381	auxpass	are,altered
R2000	T3567	T3566	advmod	likely,reflects
R2001	T3568	T3569	amod	dispersed,lysosomes
R2002	T3569	T3566	dobj	lysosomes,reflects
R2003	T3570	T3569	cc	or,lysosomes
R2004	T3571	T3572	amod	altered,localization
R2005	T3572	T3569	conj	localization,lysosomes
R2006	T3573	T3572	cc	and,localization
R2007	T3574	T3572	punct	/,localization
R2008	T3575	T3572	cc	or,localization
R2009	T3576	T3577	compound	epitope,availability
R201	T388	T381	punct	", ",altered
R2010	T3577	T3572	conj	availability,localization
R2011	T3578	T3579	punct	(,shown
R2012	T3579	T3562	parataxis	shown,indicating
R2013	T3580	T3579	nsubj	data,shown
R2014	T3581	T3579	neg	not,shown
R2015	T3582	T3579	punct	),shown
R2016	T3583	T3537	punct	.,were
R2017	T3585	T3586	nsubj	It,is
R2018	T3587	T3586	acomp	noteworthy,is
R2019	T3588	T3589	mark	that,exhibited
R202	T389	T381	cc	and,altered
R2020	T3589	T3586	ccomp	exhibited,is
R2021	T3590	T3591	compound	Lysotracker,dye
R2022	T3591	T3589	nsubj	dye,exhibited
R2023	T3592	T3591	punct	", ",dye
R2024	T3593	T3594	dep	which,accumulates
R2025	T3594	T3591	relcl	accumulates,dye
R2026	T3595	T3594	advmod	selectively,accumulates
R2027	T3596	T3594	prep	in,accumulates
R2028	T3597	T3598	amod	acidic,compartments
R2029	T3598	T3596	pobj	compartments,in
R203	T390	T391	nsubjpass	endocytosis,reduced
R2030	T3599	T3589	punct	", ",exhibited
R2031	T3600	T3601	det	the,reduction
R2032	T3601	T3589	dobj	reduction,exhibited
R2033	T3602	T3603	advmod	most,marked
R2034	T3603	T3601	amod	marked,reduction
R2035	T3604	T3601	prep	in,reduction
R2036	T3605	T3606	amod	lysosomal,labeling
R2037	T3606	T3604	pobj	labeling,in
R2038	T3607	T3586	punct	.,is
R2039	T3609	T3610	det	This,observation
R204	T391	T381	conj	reduced,altered
R2040	T3610	T3611	nsubj	observation,reflect
R2041	T3612	T3611	aux	may,reflect
R2042	T3613	T3614	amod	altered,pH
R2043	T3614	T3611	dobj	pH,reflect
R2044	T3615	T3614	amod	lysosomal,pH
R2045	T3616	T3614	punct	", ",pH
R2046	T3617	T3618	det	an,finding
R2047	T3618	T3614	appos	finding,pH
R2048	T3619	T3618	amod	established,finding
R2049	T3620	T3618	prep	in,finding
R205	T392	T391	auxpass	is,reduced
R2050	T3621	T3620	pobj	JNCL,in
R2051	T3622	T3623	punct	[,15
R2052	T3623	T3611	parataxis	15,reflect
R2053	T3624	T3623	nummod	10,15
R2054	T3625	T3623	punct	",",15
R2055	T3626	T3623	punct	],15
R2056	T3627	T3611	punct	.,reflect
R2057	T3632	T3631	nmod	C,o
R2058	T3633	T3630	nsubjpass	n,w
R2059	T3635	T3633	prep	i,n
R206	T393	T391	punct	.,reduced
R2060	T3636	T3637	nmod	ste,t
R2061	T3637	T3635	pobj	t,i
R2062	T3638	T3636	cc	n,ste
R2063	T3640	T3641	amod	i,t
R2064	T3645	T3646	amod	h,e
R2065	T3663	T3661	amod	o,me
R2066	T3669	T3668	amod	r,signal
R2067	T3670	T3668	nmod	ker,signal
R2068	T3671	T3670	punct	(,ker
R2069	T3672	T3670	appos	EEA1,ker
R207	T395	T396	prep	In,are
R2070	T3673	T3668	punct	),signal
R2071	T3674	T3668	acl	observed,signal
R2072	T3675	T3674	prep	by,observed
R2073	T3676	T3675	pobj	immunostaining,by
R2074	T3677	T3678	punct	(,Fig.
R2075	T3679	T3678	nummod	3,Fig.
R2076	T3680	T3678	punct	),Fig.
R2077	T3681	T3667	punct	", ",altered
R2078	T3682	T3683	compound	fluid,phase
R2079	T3683	T3685	compound	phase,endocytosis
R208	T397	T395	pobj	addition,In
R2080	T3684	T3683	punct	-,phase
R2081	T3685	T3667	nsubjpass	endocytosis,altered
R2082	T3686	T3667	auxpass	was,altered
R2083	T3687	T3667	advmod	also,altered
R2084	T3688	T3667	prep	in,altered
R2085	T3689	T3690	amod	homozygous,cells
R2086	T3690	T3688	pobj	cells,in
R2087	T3691	T3690	nmod	CbCln3Δex7,cells
R2088	T3692	T3691	punct	/,CbCln3Δex7
R2089	T3693	T3691	nummod	8,CbCln3Δex7
R209	T398	T396	punct	", ",are
R2090	T3694	T3667	punct	", ",altered
R2091	T3695	T3696	mark	as,measured
R2092	T3696	T3667	advcl	measured,altered
R2093	T3697	T3696	agent	by,measured
R2094	T3698	T3699	compound	dextran,FITC
R2095	T3699	T3701	compound	FITC,uptake
R2096	T3700	T3699	punct	-,FITC
R2097	T3701	T3697	pobj	uptake,by
R2098	T3702	T3703	punct	(,Fig.
R2099	T3703	T3667	parataxis	Fig.,altered
R21	T196	T197	det	a,disease
R210	T399	T396	nsubj	mitochondria,are
R2100	T3704	T3703	nummod	7,Fig.
R2101	T3705	T3703	punct	),Fig.
R2102	T3706	T3667	punct	.,altered
R2103	T3708	T3709	prep	Following,displayed
R2104	T3710	T3711	det	a,incubation
R2105	T3711	T3708	pobj	incubation,Following
R2106	T3712	T3713	nummod	15,minute
R2107	T3713	T3711	compound	minute,incubation
R2108	T3714	T3713	punct	-,minute
R2109	T3715	T3711	prep	in,incubation
R211	T400	T401	advmod	abnormally,elongated
R2110	T3716	T3715	pobj	media,in
R2111	T3717	T3716	acl	containing,media
R2112	T3718	T3719	compound	dextran,FITC
R2113	T3719	T3717	dobj	FITC,containing
R2114	T3720	T3719	punct	-,FITC
R2115	T3721	T3709	punct	", ",displayed
R2116	T3722	T3723	amod	wild,type
R2117	T3723	T3725	nmod	type,cells
R2118	T3724	T3723	punct	-,type
R2119	T3725	T3709	nsubj	cells,displayed
R212	T401	T396	acomp	elongated,are
R2120	T3726	T3723	cc	and,type
R2121	T3727	T3723	conj	heterozygote,type
R2122	T3728	T3729	advmod	brightly,stained
R2123	T3729	T3730	amod	stained,vesicles
R2124	T3730	T3709	dobj	vesicles,displayed
R2125	T3731	T3730	punct	", ",vesicles
R2126	T3732	T3730	amod	large,vesicles
R2127	T3733	T3730	amod	endocytic,vesicles
R2128	T3734	T3735	dep	that,clustered
R2129	T3735	T3730	relcl	clustered,vesicles
R213	T402	T396	punct	", ",are
R2130	T3736	T3735	auxpass	were,clustered
R2131	T3737	T3735	prep	in,clustered
R2132	T3738	T3739	det	the,region
R2133	T3739	T3737	pobj	region,in
R2134	T3740	T3739	amod	perinuclear,region
R2135	T3741	T3709	punct	.,displayed
R2136	T3743	T3744	advmod	However,stained
R2137	T3745	T3744	punct	", ",stained
R2138	T3746	T3747	amod	homozygous,cells
R2139	T3747	T3744	nsubjpass	cells,stained
R214	T403	T404	amod	cellular,levels
R2140	T3748	T3747	nmod	CbCln3Δex7,cells
R2141	T3749	T3748	punct	/,CbCln3Δex7
R2142	T3750	T3748	nummod	8,CbCln3Δex7
R2143	T3751	T3744	auxpass	were,stained
R2144	T3752	T3753	advmod	less,brightly
R2145	T3753	T3744	advmod	brightly,stained
R2146	T3754	T3744	prep	with,stained
R2147	T3755	T3756	amod	most,signal
R2148	T3756	T3760	nsubj	signal,localizing
R2149	T3757	T3758	compound	dextran,FITC
R215	T404	T406	nsubjpass	levels,decreased
R2150	T3758	T3756	compound	FITC,signal
R2151	T3759	T3758	punct	-,FITC
R2152	T3760	T3754	pcomp	localizing,with
R2153	T3761	T3760	prep	to,localizing
R2154	T3762	T3763	amod	smaller,vesicles
R2155	T3763	T3761	pobj	vesicles,to
R2156	T3764	T3763	acl	scattered,vesicles
R2157	T3765	T3764	prep	throughout,scattered
R2158	T3766	T3767	det	the,cytoplasm
R2159	T3767	T3765	pobj	cytoplasm,throughout
R216	T405	T404	compound	ATP,levels
R2160	T3768	T3767	prep	of,cytoplasm
R2161	T3769	T3770	det	the,cell
R2162	T3770	T3768	pobj	cell,of
R2163	T3771	T3744	punct	.,stained
R2164	T3778	T3776	nmod	l,l
R2165	T3780	T3779	punct	", ",y
R2166	T3792	T3793	amod	u,n
R2167	T3793	T3794	nmod	n,c
R2168	T3808	T3807	amod	hondrial,protein
R2169	T3810	T3811	poss	its,turnover
R217	T406	T396	conj	decreased,are
R2170	T3811	T3812	nsubj	turnover,proceeds
R2171	T3813	T3812	prep	through,proceeds
R2172	T3814	T3815	amod	autophagic,engulfment
R2173	T3815	T3813	pobj	engulfment,through
R2174	T3816	T3815	prep	of,engulfment
R2175	T3817	T3816	pobj	mitochondria,of
R2176	T3818	T3819	punct	[,13
R2177	T3819	T3812	parataxis	13,proceeds
R2178	T3820	T3819	punct	],13
R2179	T3821	T3806	punct	", ",analyzed
R218	T407	T406	auxpass	are,decreased
R2180	T3822	T3806	nsubj	we,analyzed
R2181	T3823	T3824	amod	homozygous,CbCln3Δex7
R2182	T3824	T3806	dobj	CbCln3Δex7,analyzed
R2183	T3825	T3824	punct	/,CbCln3Δex7
R2184	T3826	T3824	nummod	8,CbCln3Δex7
R2185	T3827	T3828	npadvmod	cell,mitochondrial
R2186	T3828	T3824	amod	mitochondrial,CbCln3Δex7
R2187	T3829	T3824	appos	morphology,CbCln3Δex7
R2188	T3830	T3829	cc	and,morphology
R2189	T3831	T3829	conj	function,morphology
R219	T408	T406	punct	", ",decreased
R2190	T3832	T3806	punct	.,analyzed
R2191	T3834	T3835	amod	Mitochondrial,distribution
R2192	T3835	T3836	nsubj	distribution,was
R2193	T3836	T3843	ccomp	was,appeared
R2194	T3837	T3835	prep	in,distribution
R2195	T3838	T3839	amod	homozygous,cells
R2196	T3839	T3837	pobj	cells,in
R2197	T3840	T3839	nmod	CbCln3Δex7,cells
R2198	T3841	T3840	punct	/,CbCln3Δex7
R2199	T3842	T3840	nummod	8,CbCln3Δex7
R22	T197	T195	attr	disease,is
R220	T409	T406	cc	and,decreased
R2200	T3844	T3836	acomp	indistinguishable,was
R2201	T3845	T3844	prep	from,indistinguishable
R2202	T3846	T3847	amod	wild,type
R2203	T3847	T3849	nmod	type,cells
R2204	T3848	T3847	punct	-,type
R2205	T3849	T3845	pobj	cells,from
R2206	T3850	T3847	cc	and,type
R2207	T3851	T3847	conj	heterozygous,type
R2208	T3852	T3843	punct	;,appeared
R2209	T3853	T3843	advmod	however,appeared
R221	T410	T411	nsubjpass	survival,reduced
R2210	T3854	T3843	punct	", ",appeared
R2211	T3855	T3856	amod	homozygous,mitochondria
R2212	T3856	T3843	nsubj	mitochondria,appeared
R2213	T3857	T3856	nmod	CbCln3Δex7,mitochondria
R2214	T3858	T3857	punct	/,CbCln3Δex7
R2215	T3859	T3857	nummod	8,CbCln3Δex7
R2216	T3860	T3861	advmod	more,elongated
R2217	T3861	T3843	oprd	elongated,appeared
R2218	T3862	T3861	prep	by,elongated
R2219	T3863	T3864	compound	grp75,immunostaining
R222	T411	T406	conj	reduced,decreased
R2220	T3864	T3862	pobj	immunostaining,by
R2221	T3865	T3864	compound	marker,immunostaining
R2222	T3866	T3864	cc	and,immunostaining
R2223	T3867	T3868	compound	TEM,analysis
R2224	T3868	T3864	conj	analysis,immunostaining
R2225	T3869	T3870	punct	(,8a
R2226	T3870	T3843	parataxis	8a,appeared
R2227	T3871	T3870	compound	Fig.,8a
R2228	T3872	T3870	punct	),8a
R2229	T3873	T3843	punct	.,appeared
R223	T412	T410	prep	following,survival
R2230	T3875	T3876	nummod	72,%
R2231	T3876	T3877	nsubj	%,were
R2232	T3878	T3876	prep	of,%
R2233	T3879	T3880	amod	homozygous,mitochondria
R2234	T3880	T3878	pobj	mitochondria,of
R2235	T3881	T3880	compound	mutant,mitochondria
R2236	T3882	T3883	amod	greater,0.6
R2237	T3883	T3885	nummod	0.6,μm
R2238	T3884	T3883	quantmod	than,0.6
R2239	T3885	T3877	attr	μm,were
R224	T413	T414	amod	oxidative,stress
R2240	T3886	T3885	prep	in,μm
R2241	T3887	T3886	pobj	length,in
R2242	T3888	T3889	punct	(,μm
R2243	T3889	T3877	parataxis	μm,were
R2244	T3890	T3889	nsubj	range,μm
R2245	T3891	T3889	punct	=,μm
R2246	T3892	T3889	nummod	0.26,μm
R2247	T3893	T3889	prep	to,μm
R2248	T3894	T3895	nummod	2.75,μm
R2249	T3895	T3893	pobj	μm,to
R225	T414	T412	pobj	stress,following
R2250	T3896	T3889	punct	),μm
R2251	T3897	T3877	punct	", ",were
R2252	T3898	T3899	mark	while,reached
R2253	T3899	T3877	advcl	reached,were
R2254	T3900	T3901	amod	fewer,mitochondria
R2255	T3901	T3899	nsubj	mitochondria,reached
R2256	T3902	T3903	amod	wild,type
R2257	T3903	T3901	compound	type,mitochondria
R2258	T3904	T3903	punct	-,type
R2259	T3905	T3906	punct	(,%
R226	T415	T411	auxpass	is,reduced
R2260	T3906	T3901	parataxis	%,mitochondria
R2261	T3907	T3906	nummod	51,%
R2262	T3908	T3906	punct	),%
R2263	T3909	T3910	det	this,length
R2264	T3910	T3899	dobj	length,reached
R2265	T3911	T3912	punct	(,μm
R2266	T3912	T3899	parataxis	μm,reached
R2267	T3913	T3912	nsubj	range,μm
R2268	T3914	T3912	punct	=,μm
R2269	T3915	T3912	nummod	0.15,μm
R227	T416	T396	punct	.,are
R2270	T3916	T3912	prep	to,μm
R2271	T3917	T3918	nummod	2.29,μm
R2272	T3918	T3916	pobj	μm,to
R2273	T3919	T3912	punct	),μm
R2274	T3920	T3877	punct	.,were
R2275	T3922	T3923	amod	Mitochondrial,width
R2276	T3923	T3924	nsubjpass	width,altered
R2277	T3925	T3924	auxpass	was,altered
R2278	T3926	T3924	neg	not,altered
R2279	T3927	T3924	prep	in,altered
R228	T420	T421	det	These,findings
R2280	T3928	T3929	amod	homozygous,cells
R2281	T3929	T3927	pobj	cells,in
R2282	T3930	T3929	nmod	CbCln3Δex7,cells
R2283	T3931	T3930	punct	/,CbCln3Δex7
R2284	T3932	T3930	nummod	8,CbCln3Δex7
R2285	T3933	T3934	punct	(,shown
R2286	T3934	T3924	parataxis	shown,altered
R2287	T3935	T3934	nsubj	data,shown
R2288	T3936	T3934	neg	not,shown
R2289	T3937	T3934	punct	),shown
R229	T421	T422	nsubj	findings,reveal
R2290	T3938	T3924	punct	.,altered
R2291	T3940	T3941	advmod	Moreover,had
R2292	T3942	T3941	punct	", ",had
R2293	T3943	T3941	prep	compared,had
R2294	T3944	T3943	prep	to,compared
R2295	T3945	T3946	amod	wild,type
R2296	T3946	T3948	nmod	type,cells
R2297	T3947	T3946	punct	-,type
R2298	T3948	T3944	pobj	cells,to
R2299	T3949	T3946	cc	or,type
R23	T198	T199	advmod	recessively,inherited
R230	T423	T424	mark	that,is
R2300	T3950	T3946	conj	heterozygous,type
R2301	T3951	T3941	punct	", ",had
R2302	T3952	T3953	amod	homozygous,cells
R2303	T3953	T3941	nsubj	cells,had
R2304	T3954	T3953	nmod	CbCln3Δex7,cells
R2305	T3955	T3954	punct	/,CbCln3Δex7
R2306	T3956	T3954	nummod	8,CbCln3Δex7
R2307	T3957	T3958	advmod	significantly,reduced
R2308	T3958	T3959	amod	reduced,levels
R2309	T3959	T3941	dobj	levels,had
R231	T424	T422	ccomp	is,reveal
R2310	T3960	T3961	amod	cellular,ATP
R2311	T3961	T3959	compound	ATP,levels
R2312	T3962	T3963	punct	(,Fig.
R2313	T3963	T3941	parataxis	Fig.,had
R2314	T3964	T3965	npadvmod	1.3,less
R2315	T3965	T3963	amod	less,Fig.
R2316	T3966	T3964	advmod	fold,1.3
R2317	T3967	T3963	punct	", ",Fig.
R2318	T3968	T3963	nummod	8b,Fig.
R2319	T3969	T3963	punct	),Fig.
R232	T425	T424	nsubj	battenin,is
R2320	T3970	T3941	cc	and,had
R2321	T3971	T3941	conj	exhibited,had
R2322	T3972	T3973	amod	reduced,survival
R2323	T3973	T3971	dobj	survival,exhibited
R2324	T3974	T3971	prep	following,exhibited
R2325	T3975	T3976	compound	hydrogen,peroxide
R2326	T3976	T3977	compound	peroxide,treatment
R2327	T3977	T3974	pobj	treatment,following
R2328	T3978	T3979	punct	(,8c
R2329	T3979	T3971	parataxis	8c,exhibited
R233	T426	T424	acomp	required,is
R2330	T3980	T3981	punct	~,50
R2331	T3981	T3982	nummod	50,%
R2332	T3982	T3979	dep	%,8c
R2333	T3983	T3982	prep	of,%
R2334	T3984	T3985	amod	wild,type
R2335	T3985	T3987	compound	type,survival
R2336	T3986	T3985	punct	-,type
R2337	T3987	T3983	pobj	survival,of
R2338	T3988	T3979	punct	", ",8c
R2339	T3989	T3979	compound	Fig.,8c
R234	T427	T426	prep	for,required
R2340	T3990	T3979	punct	),8c
R2341	T3991	T3941	punct	", ",had
R2342	T3992	T3941	advcl	suggesting,had
R2343	T3993	T3994	amod	impaired,metabolism
R2344	T3994	T3992	dobj	metabolism,suggesting
R2345	T3995	T3994	compound	energy,metabolism
R2346	T3996	T3994	cc	and,metabolism
R2347	T3997	T3998	amod	oxidative,response
R2348	T3998	T3994	conj	response,metabolism
R2349	T3999	T3998	compound	stress,response
R235	T428	T429	amod	intracellular,trafficking
R2350	T4000	T3941	punct	.,had
R2351	T4002	T4003	advcl	Taken,support
R2352	T4004	T4002	advmod	together,Taken
R2353	T4005	T4003	punct	", ",support
R2354	T4006	T4007	det	these,data
R2355	T4007	T4003	nsubj	data,support
R2356	T4008	T4009	amod	impaired,function
R2357	T4009	T4003	dobj	function,support
R2358	T4010	T4009	amod	mitochondrial,function
R2359	T4011	T4009	prep	in,function
R236	T429	T427	pobj	trafficking,for
R2360	T4012	T4013	amod	homozygous,cells
R2361	T4013	T4011	pobj	cells,in
R2362	T4014	T4013	nmod	CbCln3Δex7,cells
R2363	T4015	T4014	punct	/,CbCln3Δex7
R2364	T4016	T4014	nummod	8,CbCln3Δex7
R2365	T4017	T4003	punct	.,support
R237	T430	T429	compound	membrane,trafficking
R238	T431	T429	cc	and,trafficking
R2382	T4415	T4416	nmod	CbCln3Δex7,cells
R2383	T4416	T4421	nsubj	cells,represent
R2384	T4417	T4415	punct	/,CbCln3Δex7
R2385	T4418	T4415	nummod	8,CbCln3Δex7
R2386	T4419	T4420	amod	cerebellar,precursor
R2387	T4420	T4416	compound	precursor,cells
R2388	T4422	T4423	det	the,model
R2389	T4423	T4421	dobj	model,represent
R239	T432	T433	amod	mitochondrial,function
R2390	T4424	T4423	amod	first,model
R2391	T4425	T4426	advmod	genetically,accurate
R2392	T4426	T4423	amod	accurate,model
R2393	T4427	T4428	npadvmod	neuron,derived
R2394	T4428	T4423	amod	derived,model
R2395	T4429	T4428	punct	-,derived
R2396	T4430	T4423	compound	culture,model
R2397	T4431	T4423	prep	of,model
R2398	T4432	T4431	pobj	JNCL,of
R2399	T4433	T4421	punct	.,represent
R24	T199	T197	amod	inherited,disease
R240	T433	T429	conj	function,trafficking
R2400	T4435	T4436	amod	Homozygous,cells
R2401	T4436	T4440	nsubj	cells,express
R2402	T4437	T4436	nmod	CbCln3Δex7,cells
R2403	T4438	T4437	punct	/,CbCln3Δex7
R2404	T4439	T4437	nummod	8,CbCln3Δex7
R2405	T4441	T4442	compound	mutant,battenin
R2406	T4442	T4440	dobj	battenin,express
R2407	T4443	T4442	cc	and,battenin
R2408	T4444	T4445	nmod	JNCL,hallmark
R2409	T4445	T4447	nmod	hallmark,accumulation
R241	T434	T422	punct	.,reveal
R2410	T4446	T4445	punct	-,hallmark
R2411	T4447	T4442	conj	accumulation,battenin
R2412	T4448	T4447	amod	mitochondrial,accumulation
R2413	T4449	T4447	compound	ATPase,accumulation
R2414	T4450	T4451	compound	subunit,c
R2415	T4451	T4447	compound	c,accumulation
R2416	T4452	T4440	punct	", ",express
R2417	T4453	T4440	prep	upon,express
R2418	T4454	T4453	pobj	aging,upon
R2419	T4455	T4454	prep	of,aging
R242	T436	T437	advmod	Moreover,are
R2420	T4456	T4455	pobj	cells,of
R2421	T4457	T4456	prep	at,cells
R2422	T4458	T4457	pobj	confluency,at
R2423	T4459	T4440	punct	.,express
R2424	T4461	T4462	advmod	Moreover,is
R2425	T4463	T4462	punct	", ",is
R2426	T4464	T4462	nsubj	this,is
R2427	T4465	T4466	det	the,study
R2428	T4466	T4462	attr	study,is
R2429	T4467	T4466	amod	first,study
R243	T438	T437	punct	", ",are
R2430	T4468	T4469	aux	to,indicate
R2431	T4469	T4466	advcl	indicate,study
R2432	T4470	T4471	amod	recessive,defects
R2433	T4471	T4469	dobj	defects,indicate
R2434	T4472	T4473	amod	endosomal,lysosomal
R2435	T4473	T4475	amod	lysosomal,membrane
R2436	T4474	T4473	punct	/,lysosomal
R2437	T4475	T4471	compound	membrane,defects
R2438	T4476	T4471	compound	trafficking,defects
R2439	T4477	T4471	cc	and,defects
R244	T439	T440	det	these,deficiencies
R2440	T4478	T4479	amod	mitochondrial,dysfunction
R2441	T4479	T4471	conj	dysfunction,defects
R2442	T4480	T4481	dep	that,precedes
R2443	T4481	T4471	relcl	precedes,defects
R2444	T4482	T4483	compound	subunit,c
R2445	T4483	T4484	compound	c,deposition
R2446	T4484	T4481	dobj	deposition,precedes
R2447	T4485	T4481	prep	in,precedes
R2448	T4486	T4487	det	an,model
R2449	T4487	T4485	pobj	model,in
R245	T440	T437	nsubj	deficiencies,are
R2450	T4488	T4487	amod	accurate,model
R2451	T4489	T4487	compound	JNCL,model
R2452	T4490	T4462	punct	.,is
R2453	T4492	T4493	advmod	Importantly,are
R2454	T4494	T4493	punct	", ",are
R2455	T4495	T4496	det	these,defects
R2456	T4496	T4493	nsubj	defects,are
R2457	T4497	T4493	acomp	likely,are
R2458	T4498	T4499	aux	to,be
R2459	T4499	T4497	xcomp	be,likely
R246	T441	T437	acomp	likely,are
R2460	T4500	T4501	amod	early,events
R2461	T4501	T4499	attr	events,be
R2462	T4502	T4501	prep	in,events
R2463	T4503	T4504	det	the,process
R2464	T4504	T4502	pobj	process,in
R2465	T4505	T4504	compound	JNCL,process
R2466	T4506	T4504	compound	disease,process
R2467	T4507	T4493	cc	and,are
R2468	T4508	T4509	aux	may,impact
R2469	T4509	T4493	conj	impact,are
R247	T442	T443	aux	to,be
R2470	T4510	T4509	advmod	particularly,impact
R2471	T4511	T4512	amod	neuronal,function
R2472	T4512	T4509	dobj	function,impact
R2473	T4513	T4493	punct	.,are
R2474	T4515	T4516	amod	Abnormal,D
R2475	T4516	T4518	nsubj	D,reflects
R2476	T4517	T4516	compound	cathepsin,D
R2477	T4519	T4516	appos	localization,D
R2478	T4520	T4519	cc	and,localization
R2479	T4521	T4519	conj	processing,localization
R248	T443	T441	xcomp	be,likely
R2480	T4522	T4516	prep	in,D
R2481	T4523	T4524	amod	homozygous,cells
R2482	T4524	T4522	pobj	cells,in
R2483	T4525	T4524	nmod	CbCln3Δex7,cells
R2484	T4526	T4525	punct	/,CbCln3Δex7
R2485	T4527	T4525	nummod	8,CbCln3Δex7
R2486	T4528	T4524	cc	and,cells
R2487	T4529	T4530	nmod	Cln3Δex7,mice
R2488	T4530	T4524	conj	mice,cells
R2489	T4531	T4529	punct	/,Cln3Δex7
R249	T444	T445	amod	early,events
R2490	T4532	T4529	nummod	8,Cln3Δex7
R2491	T4533	T4518	advmod	likely,reflects
R2492	T4534	T4535	amod	altered,trafficking
R2493	T4535	T4518	dobj	trafficking,reflects
R2494	T4536	T4535	amod	vesicular,trafficking
R2495	T4537	T4535	cc	and,trafficking
R2496	T4538	T4535	punct	/,trafficking
R2497	T4539	T4535	cc	or,trafficking
R2498	T4540	T4541	amod	lysosomal,pH
R2499	T4541	T4535	conj	pH,trafficking
R25	T200	T197	punct	", ",disease
R250	T445	T443	attr	events,be
R2500	T4542	T4535	punct	", ",trafficking
R2501	T4543	T4544	dep	which,known
R2502	T4544	T4535	relcl	known,trafficking
R2503	T4545	T4544	auxpass	is,known
R2504	T4546	T4547	aux	to,impact
R2505	T4547	T4544	xcomp	impact,known
R2506	T4548	T4549	compound	cathepsin,D
R2507	T4549	T4550	compound	D,processing
R2508	T4550	T4547	dobj	processing,impact
R2509	T4551	T4552	punct	[,16
R251	T446	T445	prep	in,events
R2510	T4552	T4544	parataxis	16,known
R2511	T4553	T4552	nummod	14,16
R2512	T4554	T4552	punct	",",16
R2513	T4555	T4552	punct	],16
R2514	T4556	T4518	punct	.,reflects
R2515	T4558	T4559	advmod	Indeed,altered
R2516	T4560	T4559	punct	", ",altered
R2517	T4561	T4562	compound	CLN3,overexpression
R2518	T4562	T4559	nsubj	overexpression,altered
R2519	T4563	T4562	prep	in,overexpression
R252	T447	T448	det	the,process
R2520	T4564	T4565	nmod	HEK,cells
R2521	T4565	T4563	pobj	cells,in
R2522	T4566	T4564	punct	-,HEK
R2523	T4567	T4564	nummod	293,HEK
R2524	T4568	T4569	amod	lysosomal,pH
R2525	T4569	T4570	nmod	pH,processing
R2526	T4570	T4559	dobj	processing,altered
R2527	T4571	T4569	cc	and,pH
R2528	T4572	T4573	compound	cathepsin,D
R2529	T4573	T4569	conj	D,pH
R253	T448	T446	pobj	process,in
R2530	T4574	T4575	punct	[,17
R2531	T4575	T4559	parataxis	17,altered
R2532	T4576	T4575	punct	],17
R2533	T4577	T4559	punct	", ",altered
R2534	T4578	T4559	cc	and,altered
R2535	T4579	T4580	amod	lysosomal,pH
R2536	T4580	T4581	compound	pH,homeostasis
R2537	T4581	T4582	nsubjpass	homeostasis,disrupted
R2538	T4582	T4559	conj	disrupted,altered
R2539	T4583	T4582	auxpass	is,disrupted
R254	T449	T448	compound	JNCL,process
R2540	T4584	T4582	prep	in,disrupted
R2541	T4585	T4584	pobj	JNCL,in
R2542	T4586	T4587	punct	[,15
R2543	T4587	T4582	parataxis	15,disrupted
R2544	T4588	T4587	nummod	10,15
R2545	T4589	T4587	punct	",",15
R2546	T4590	T4587	punct	],15
R2547	T4591	T4582	punct	.,disrupted
R2548	T4593	T4594	nsubj	It,is
R2549	T4595	T4594	acomp	noteworthy,is
R255	T450	T448	compound	disease,process
R2550	T4596	T4597	mark	that,altered
R2551	T4597	T4594	ccomp	altered,is
R2552	T4598	T4599	compound	cathepsin,B
R2553	T4599	T4597	nsubjpass	B,altered
R2554	T4600	T4599	cc	and,B
R2555	T4601	T4602	det	the,enzyme
R2556	T4602	T4599	conj	enzyme,B
R2557	T4603	T4604	npadvmod	CLN2,encoded
R2558	T4604	T4602	amod	encoded,enzyme
R2559	T4605	T4604	punct	-,encoded
R256	T451	T437	cc	and,are
R2560	T4606	T4602	punct	", ",enzyme
R2561	T4607	T4602	appos	TPPI,enzyme
R2562	T4608	T4597	punct	", ",altered
R2563	T4609	T4597	auxpass	are,altered
R2564	T4610	T4597	advmod	also,altered
R2565	T4611	T4597	prep	in,altered
R2566	T4612	T4611	pobj	JNCL,in
R2567	T4613	T4614	punct	[,18
R2568	T4614	T4597	parataxis	18,altered
R2569	T4615	T4616	punct	-,20
R257	T452	T453	aux	may,impact
R2570	T4616	T4614	prep	20,18
R2571	T4617	T4614	punct	],18
R2572	T4618	T4594	punct	.,is
R2573	T4620	T4621	advmod	Nevertheless,appear
R2574	T4622	T4621	punct	", ",appear
R2575	T4623	T4621	prep	despite,appear
R2576	T4624	T4625	det	the,alterations
R2577	T4625	T4623	pobj	alterations,despite
R2578	T4626	T4627	compound	cathepsin,D
R2579	T4627	T4625	compound	D,alterations
R258	T453	T437	conj	impact,are
R2580	T4628	T4625	compound	protein,alterations
R2581	T4629	T4630	dep	that,observed
R2582	T4630	T4625	relcl	observed,alterations
R2583	T4631	T4630	auxpass	are,observed
R2584	T4632	T4630	prep	in,observed
R2585	T4633	T4634	amod	homozygous,cells
R2586	T4634	T4632	pobj	cells,in
R2587	T4635	T4634	nmod	CbCln3Δex7,cells
R2588	T4636	T4635	punct	/,CbCln3Δex7
R2589	T4637	T4635	nummod	8,CbCln3Δex7
R259	T454	T453	advmod	particularly,impact
R2590	T4638	T4621	punct	", ",appear
R2591	T4639	T4640	nmod	cathepsin,D
R2592	T4640	T4641	nmod	D,activity
R2593	T4641	T4621	nsubj	activity,appear
R2594	T4642	T4641	amod	enzymatic,activity
R2595	T4643	T4621	aux	does,appear
R2596	T4644	T4621	neg	not,appear
R2597	T4645	T4646	aux	to,reduced
R2598	T4646	T4621	xcomp	reduced,appear
R2599	T4647	T4646	auxpass	be,reduced
R26	T201	T202	nmod	childhood,onset
R260	T455	T456	amod	neuronal,survival
R2600	T4648	T4621	punct	.,appear
R2601	T4650	T4651	advmod	Thus,is
R2602	T4652	T4651	punct	", ",is
R2603	T4653	T4654	amod	decreased,activity
R2604	T4654	T4651	nsubj	activity,is
R2605	T4655	T4656	compound	cathepsin,D
R2606	T4656	T4654	compound	D,activity
R2607	T4657	T4651	acomp	unlikely,is
R2608	T4658	T4659	aux	to,account
R2609	T4659	T4657	xcomp	account,unlikely
R261	T456	T453	dobj	survival,impact
R2610	T4660	T4659	prep	for,account
R2611	T4661	T4662	compound	subunit,c
R2612	T4662	T4663	compound	c,accumulation
R2613	T4663	T4660	pobj	accumulation,for
R2614	T4664	T4659	prep	in,account
R2615	T4665	T4664	pobj	JNCL,in
R2616	T4666	T4651	punct	.,is
R2617	T4668	T4669	nsubj	Aging,is
R2618	T4670	T4668	prep	of,Aging
R2619	T4671	T4672	amod	homozygous,cells
R262	T457	T437	punct	.,are
R2620	T4672	T4670	pobj	cells,of
R2621	T4673	T4672	nmod	CbCln3Δex7,cells
R2622	T4674	T4673	punct	/,CbCln3Δex7
R2623	T4675	T4673	nummod	8,CbCln3Δex7
R2624	T4676	T4668	prep	at,Aging
R2625	T4677	T4676	pobj	confluency,at
R2626	T4678	T4669	acomp	necessary,is
R2627	T4679	T4680	aux	to,induce
R2628	T4680	T4678	xcomp	induce,necessary
R2629	T4681	T4682	advmod	significantly,accumulated
R263	T187	T185	pobj	lipofuscinosis,of
R2630	T4682	T4683	amod	accumulated,protein
R2631	T4683	T4680	dobj	protein,induce
R2632	T4684	T4685	compound	subunit,c
R2633	T4685	T4683	compound	c,protein
R2634	T4686	T4669	punct	.,is
R2635	T4688	T4689	advmod	However,precede
R2636	T4690	T4689	punct	", ",precede
R2637	T4691	T4692	compound	membrane,organelle
R2638	T4692	T4693	compound	organelle,disruptions
R2639	T4693	T4689	nsubj	disruptions,precede
R2640	T4694	T4695	compound	subunit,c
R2641	T4695	T4696	compound	c,accumulation
R2642	T4696	T4689	dobj	accumulation,precede
R2643	T4697	T4689	prep	in,precede
R2644	T4698	T4699	amod	homozygous,cells
R2645	T4699	T4697	pobj	cells,in
R2646	T4700	T4699	nmod	CbCln3Δex7,cells
R2647	T4701	T4700	punct	/,CbCln3Δex7
R2648	T4702	T4700	nummod	8,CbCln3Δex7
R2649	T4703	T4689	punct	", ",precede
R2650	T4704	T4705	mark	since,observed
R2651	T4705	T4689	advcl	observed,precede
R2652	T4706	T4705	nsubjpass	they,observed
R2653	T4707	T4705	auxpass	are,observed
R2654	T4708	T4705	prep	without,observed
R2655	T4709	T4708	pobj	aging,without
R2656	T4710	T4705	prep	at,observed
R2657	T4711	T4710	pobj	confluency,at
R2658	T4712	T4689	punct	.,precede
R2659	T4714	T4715	amod	Lysosomal,size
R266	T696	T697	amod	Juvenile,lipofuscinosis
R2660	T4715	T4718	nsubjpass	size,altered
R2661	T4716	T4714	cc	and,Lysosomal
R2662	T4717	T4714	conj	endosomal,Lysosomal
R2663	T4719	T4715	cc	and,size
R2664	T4720	T4715	conj	distribution,size
R2665	T4721	T4718	auxpass	are,altered
R2666	T4722	T4718	punct	", ",altered
R2667	T4723	T4718	cc	and,altered
R2668	T4724	T4725	nsubj	mitochondria,are
R2669	T4725	T4718	conj	are,altered
R267	T697	T700	nsubj	lipofuscinosis,is
R2670	T4726	T4727	advmod	abnormally,elongated
R2671	T4727	T4725	acomp	elongated,are
R2672	T4728	T4727	cc	and,elongated
R2673	T4729	T4730	advmod	functionally,compromised
R2674	T4730	T4727	conj	compromised,elongated
R2675	T4731	T4725	prep	in,are
R2676	T4732	T4733	amod	sub-confluent,cultures
R2677	T4733	T4731	pobj	cultures,in
R2678	T4734	T4733	amod	homozygous,cultures
R2679	T4735	T4733	nmod	CbCln3Δex7,cultures
R268	T698	T697	amod	neuronal,lipofuscinosis
R2680	T4736	T4735	punct	/,CbCln3Δex7
R2681	T4737	T4735	nummod	8,CbCln3Δex7
R2682	T4738	T4725	punct	.,are
R2683	T4740	T4741	det	These,observations
R2684	T4741	T4742	nsubj	observations,argue
R2685	T4743	T4744	mark	that,result
R2686	T4744	T4742	ccomp	result,argue
R2687	T4745	T4746	compound	membrane,trafficking
R2688	T4746	T4747	compound	trafficking,defects
R2689	T4747	T4744	nsubj	defects,result
R269	T699	T697	compound	ceroid,lipofuscinosis
R2690	T4748	T4744	aux	do,result
R2691	T4749	T4744	neg	not,result
R2692	T4750	T4744	prep	from,result
R2693	T4751	T4752	amod	excessive,accumulation
R2694	T4752	T4755	nsubj	accumulation,compromising
R2695	T4753	T4754	compound	subunit,c
R2696	T4754	T4752	compound	c,accumulation
R2697	T4755	T4750	pcomp	compromising,from
R2698	T4756	T4757	det	the,lysosome
R2699	T4757	T4755	dobj	lysosome,compromising
R27	T202	T197	nmod	onset,disease
R270	T701	T697	punct	(,lipofuscinosis
R2700	T4758	T4744	punct	", ",result
R2701	T4759	T4744	cc	but,result
R2702	T4760	T4759	advmod	rather,but
R2703	T4761	T4744	conj	are,result
R2704	T4762	T4763	amod	early,events
R2705	T4763	T4761	attr	events,are
R2706	T4764	T4763	prep	in,events
R2707	T4765	T4766	det	the,process
R2708	T4766	T4764	pobj	process,in
R2709	T4767	T4766	compound	disease,process
R271	T702	T697	appos	JNCL,lipofuscinosis
R2710	T4768	T4763	acl	preceding,events
R2711	T4769	T4770	compound	subunit,c
R2712	T4770	T4771	compound	c,accumulation
R2713	T4771	T4768	dobj	accumulation,preceding
R2714	T4772	T4742	punct	.,argue
R2715	T4774	T4775	amod	Mitochondrial,abnormalities
R2716	T4775	T4776	nsubj	abnormalities,result
R2717	T4777	T4775	punct	", ",abnormalities
R2718	T4778	T4779	dep	which,reported
R2719	T4779	T4775	relcl	reported,abnormalities
R272	T703	T697	punct	),lipofuscinosis
R2720	T4780	T4779	aux	have,reported
R2721	T4781	T4779	advmod	also,reported
R2722	T4782	T4779	auxpass	been,reported
R2723	T4783	T4779	prep	in,reported
R2724	T4784	T4785	compound	JNCL,patients
R2725	T4785	T4783	pobj	patients,in
R2726	T4786	T4785	cc	and,patients
R2727	T4787	T4788	amod	other,models
R2728	T4788	T4785	conj	models,patients
R2729	T4789	T4788	compound	animal,models
R273	T704	T697	punct	", ",lipofuscinosis
R2730	T4790	T4791	punct	[,21
R2731	T4791	T4779	parataxis	21,reported
R2732	T4792	T4793	punct	-,23
R2733	T4793	T4791	prep	23,21
R2734	T4794	T4791	punct	],21
R2735	T4795	T4776	punct	", ",result
R2736	T4796	T4776	aux	may,result
R2737	T4797	T4776	prep	from,result
R2738	T4798	T4799	amod	ineffective,turnover
R2739	T4799	T4797	pobj	turnover,from
R274	T705	T697	cc	or,lipofuscinosis
R2740	T4800	T4799	prep	by,turnover
R2741	T4801	T4800	pobj	autophagy,by
R2742	T4802	T4801	punct	", ",autophagy
R2743	T4803	T4804	det	a,pathway
R2744	T4804	T4801	appos	pathway,autophagy
R2745	T4805	T4806	amod	lysosomal,targeted
R2746	T4806	T4804	amod	targeted,pathway
R2747	T4807	T4806	punct	-,targeted
R2748	T4808	T4809	punct	[,24
R2749	T4809	T4776	parataxis	24,result
R275	T706	T707	compound	Batten,disease
R2750	T4810	T4809	punct	],24
R2751	T4811	T4776	punct	.,result
R2752	T4813	T4814	advmod	Alternatively,impact
R2753	T4815	T4813	punct	", ",Alternatively
R2754	T4816	T4813	cc	or,Alternatively
R2755	T4817	T4813	conj	simultaneously,Alternatively
R2756	T4818	T4814	punct	", ",impact
R2757	T4819	T4820	compound	battenin,deficiency
R2758	T4820	T4814	nsubj	deficiency,impact
R2759	T4821	T4814	aux	may,impact
R276	T707	T697	conj	disease,lipofuscinosis
R2760	T4822	T4823	amod	mitochondrial,function
R2761	T4823	T4814	dobj	function,impact
R2762	T4824	T4823	advmod	upstream,function
R2763	T4825	T4824	prep	of,upstream
R2764	T4826	T4825	pobj	turnover,of
R2765	T4827	T4814	punct	", ",impact
R2766	T4828	T4814	advcl	affecting,impact
R2767	T4829	T4830	amod	mitochondrial,biogenesis
R2768	T4830	T4828	dobj	biogenesis,affecting
R2769	T4831	T4830	cc	and,biogenesis
R277	T708	T700	punct	", ",is
R2770	T4832	T4831	punct	/,and
R2771	T4833	T4831	cc	or,and
R2772	T4834	T4835	amod	altered,transport
R2773	T4835	T4830	conj	transport,biogenesis
R2774	T4836	T4835	cc	and,transport
R2775	T4837	T4835	conj	processing,transport
R2776	T4838	T4835	prep	of,transport
R2777	T4839	T4840	amod	mitochondrial,proteins
R2778	T4840	T4838	pobj	proteins,of
R2779	T4841	T4814	punct	.,impact
R278	T709	T710	det	a,disorder
R2780	T4843	T4844	prep	In,co-localizes
R2781	T4845	T4846	amod	wild,type
R2782	T4846	T4848	nmod	type,cells
R2783	T4847	T4846	punct	-,type
R2784	T4848	T4843	pobj	cells,In
R2785	T4849	T4848	nmod	CbCln3,cells
R2786	T4850	T4851	amod	neuronal,precursor
R2787	T4851	T4848	compound	precursor,cells
R2788	T4852	T4844	nsubj	battenin,co-localizes
R2789	T4853	T4844	advmod	primarily,co-localizes
R279	T710	T700	attr	disorder,is
R2790	T4854	T4844	prep	with,co-localizes
R2791	T4855	T4856	amod	early,endosomes
R2792	T4856	T4854	pobj	endosomes,with
R2793	T4857	T4855	cc	and,early
R2794	T4858	T4855	conj	late,early
R2795	T4859	T4844	punct	.,co-localizes
R2796	T4861	T4862	compound	Battenin,immunostaining
R2797	T4862	T4863	nsubjpass	immunostaining,reduced
R2798	T4864	T4862	prep	in,immunostaining
R2799	T4865	T4866	amod	homozygous,precursors
R28	T203	T202	punct	-,onset
R280	T711	T712	advmod	recessively,inherited
R2800	T4866	T4864	pobj	precursors,in
R2801	T4867	T4866	nmod	CbCln3Δex7,precursors
R2802	T4868	T4867	punct	/,CbCln3Δex7
R2803	T4869	T4867	nummod	8,CbCln3Δex7
R2804	T4870	T4866	amod	neuronal,precursors
R2805	T4871	T4863	auxpass	is,reduced
R2806	T4872	T4863	advmod	significantly,reduced
R2807	T4873	T4863	prep	in,reduced
R2808	T4874	T4873	pobj	abundance,in
R2809	T4875	T4863	punct	", ",reduced
R281	T712	T710	amod	inherited,disorder
R2810	T4876	T4863	cc	but,reduced
R2811	T4877	T4878	compound	mutant,signal
R2812	T4878	T4879	nsubj	signal,co-localizes
R2813	T4879	T4863	conj	co-localizes,reduced
R2814	T4880	T4879	advmod	also,co-localizes
R2815	T4881	T4879	prep	with,co-localizes
R2816	T4882	T4883	amod	endosomal,markers
R2817	T4883	T4881	pobj	markers,with
R2818	T4884	T4879	advcl	suggesting,co-localizes
R2819	T4885	T4886	compound	mutant,protein
R282	T713	T710	nmod	childhood,disorder
R2820	T4886	T4888	nsubjpass	protein,trafficked
R2821	T4887	T4886	compound	battenin,protein
R2822	T4888	T4884	advcl	trafficked,suggesting
R2823	T4889	T4886	prep	with,protein
R2824	T4890	T4891	npadvmod	C,terminal
R2825	T4891	T4893	amod	terminal,epitopes
R2826	T4892	T4891	punct	-,terminal
R2827	T4893	T4889	pobj	epitopes,with
R2828	T4894	T4888	auxpass	is,trafficked
R2829	T4895	T4888	advcl	similar,trafficked
R283	T714	T713	punct	-,childhood
R2830	T4896	T4895	prep	to,similar
R2831	T4897	T4898	amod	wild,type
R2832	T4898	T4900	compound	type,protein
R2833	T4899	T4898	punct	-,type
R2834	T4900	T4896	pobj	protein,to
R2835	T4901	T4879	punct	.,co-localizes
R2836	T4903	T4904	prep	In,reported
R2837	T4905	T4906	amod	other,studies
R2838	T4906	T4903	pobj	studies,In
R2839	T4907	T4904	punct	", ",reported
R284	T715	T713	amod	onset,childhood
R2840	T4908	T4909	nmod	CLN3,localization
R2841	T4909	T4904	nsubjpass	localization,reported
R2842	T4910	T4908	punct	/,CLN3
R2843	T4911	T4912	compound	battenin,protein
R2844	T4912	T4908	appos	protein,CLN3
R2845	T4913	T4904	aux	has,reported
R2846	T4914	T4904	auxpass	been,reported
R2847	T4915	T4916	aux	to,overlap
R2848	T4916	T4904	xcomp	overlap,reported
R2849	T4917	T4916	advmod	partially,overlap
R285	T716	T710	amod	neurodegenerative,disorder
R2850	T4918	T4916	prep	with,overlap
R2851	T4919	T4920	amod	late,endosomes
R2852	T4920	T4918	pobj	endosomes,with
R2853	T4921	T4920	cc	and,endosomes
R2854	T4922	T4920	conj	lysosomes,endosomes
R2855	T4923	T4916	prep	in,overlap
R2856	T4924	T4925	amod	non-neuronal,cells
R2857	T4925	T4923	pobj	cells,in
R2858	T4926	T4927	punct	[,7
R2859	T4927	T4916	parataxis	7,overlap
R286	T717	T710	acl	characterized,disorder
R2860	T4928	T4927	punct	],7
R2861	T4929	T4916	punct	", ",overlap
R2862	T4930	T4916	cc	and,overlap
R2863	T4931	T4916	conj	to,overlap
R2864	T4932	T4931	pobj	lysosomes,to
R2865	T4933	T4932	punct	", ",lysosomes
R2866	T4934	T4932	conj	synaptosomes,lysosomes
R2867	T4935	T4936	punct	[,8
R2868	T4936	T4934	parataxis	8,synaptosomes
R2869	T4937	T4936	punct	],8
R287	T718	T717	agent	by,characterized
R2870	T4938	T4934	cc	and,synaptosomes
R2871	T4939	T4934	conj	endosomes,synaptosomes
R2872	T4940	T4941	punct	[,25
R2873	T4941	T4939	parataxis	25,endosomes
R2874	T4942	T4941	nummod	9,25
R2875	T4943	T4941	punct	",",25
R2876	T4944	T4941	punct	],25
R2877	T4945	T4931	prep	in,to
R2878	T4946	T4945	pobj	neurons,in
R2879	T4947	T4904	punct	.,reported
R288	T719	T720	amod	progressive,blindness
R2880	T4949	T4950	det	These,data
R2881	T4950	T4951	nsubj	data,indicate
R2882	T4952	T4951	advmod	jointly,indicate
R2883	T4953	T4954	mark	that,resides
R2884	T4954	T4951	ccomp	resides,indicate
R2885	T4955	T4954	nsubj	battenin,resides
R2886	T4956	T4954	prep	in,resides
R2887	T4957	T4958	det	a,subset
R2888	T4958	T4956	pobj	subset,in
R2889	T4959	T4958	prep	of,subset
R289	T720	T718	pobj	blindness,by
R2890	T4960	T4961	amod	vesicular,compartments
R2891	T4961	T4959	pobj	compartments,of
R2892	T4962	T4961	acl	linking,compartments
R2893	T4963	T4964	amod	multiple,pathways
R2894	T4964	T4962	dobj	pathways,linking
R2895	T4965	T4966	compound	membrane,trafficking
R2896	T4966	T4964	compound	trafficking,pathways
R2897	T4967	T4954	punct	", ",resides
R2898	T4968	T4969	advmod	perhaps,functioning
R2899	T4969	T4954	advcl	functioning,resides
R29	T204	T197	amod	neurodegenerative,disease
R290	T721	T720	punct	", ",blindness
R2900	T4970	T4969	prep	in,functioning
R2901	T4971	T4972	amod	vesicular,transport
R2902	T4972	T4970	pobj	transport,in
R2903	T4973	T4972	cc	and,transport
R2904	T4974	T4973	punct	/,and
R2905	T4975	T4973	cc	or,and
R2906	T4976	T4972	conj	fusion,transport
R2907	T4977	T4951	punct	.,indicate
R2908	T4979	T4980	amod	Endocytic,targeted
R2909	T4980	T4984	amod	targeted,pathways
R291	T722	T720	conj	seizures,blindness
R2910	T4981	T4979	cc	and,Endocytic
R2911	T4982	T4979	conj	lysosomal,Endocytic
R2912	T4983	T4980	punct	-,targeted
R2913	T4984	T4985	nsubjpass	pathways,implicated
R2914	T4986	T4984	punct	", ",pathways
R2915	T4987	T4984	prep	including,pathways
R2916	T4988	T4989	amod	mitochondrial,autophagy
R2917	T4989	T4987	pobj	autophagy,including
R2918	T4990	T4985	punct	", ",implicated
R2919	T4991	T4985	auxpass	are,implicated
R292	T723	T722	punct	", ",seizures
R2920	T4992	T4985	advmod	specifically,implicated
R2921	T4993	T4985	prep	in,implicated
R2922	T4994	T4995	det	this,study
R2923	T4995	T4993	pobj	study,in
R2924	T4996	T4985	punct	.,implicated
R2925	T5047	T5048	det	The,trafficking
R2926	T5048	T5050	nsubj	trafficking,are
R2927	T5049	T5048	compound	membrane,trafficking
R2928	T5051	T5048	cc	and,trafficking
R2929	T5052	T5053	amod	mitochondrial,deficits
R293	T724	T725	nmod	motor,decline
R2930	T5053	T5048	conj	deficits,trafficking
R2931	T5054	T5048	acl	uncovered,trafficking
R2932	T5055	T5054	prep	in,uncovered
R2933	T5056	T5057	amod	homozygous,cells
R2934	T5057	T5055	pobj	cells,in
R2935	T5058	T5057	nmod	CbCln3Δex7,cells
R2936	T5059	T5058	punct	/,CbCln3Δex7
R2937	T5060	T5058	nummod	8,CbCln3Δex7
R2938	T5061	T5050	acomp	likely,are
R2939	T5062	T5063	aux	to,impact
R294	T725	T722	conj	decline,seizures
R2940	T5063	T5061	xcomp	impact,likely
R2941	T5064	T5063	advmod	particularly,impact
R2942	T5065	T5066	amod	neuronal,function
R2943	T5066	T5063	dobj	function,impact
R2944	T5067	T5050	punct	.,are
R2945	T5069	T5070	nsubj	Neurotransmission,relies
R2946	T5071	T5070	advmod	heavily,relies
R2947	T5072	T5070	prep	on,relies
R2948	T5073	T5074	compound	membrane,vesicle
R2949	T5074	T5075	compound	vesicle,transport
R295	T726	T724	cc	and,motor
R2950	T5075	T5072	pobj	transport,on
R2951	T5076	T5070	punct	", ",relies
R2952	T5077	T5070	cc	and,relies
R2953	T5078	T5079	det	a,metabolism
R2954	T5079	T5083	nsubj	metabolism,sensitize
R2955	T5080	T5081	amod	high,energy
R2956	T5081	T5079	compound	energy,metabolism
R2957	T5082	T5081	punct	-,energy
R2958	T5083	T5070	conj	sensitize,relies
R2959	T5084	T5083	aux	may,sensitize
R296	T727	T724	conj	cognitive,motor
R2960	T5085	T5083	advmod	further,sensitize
R2961	T5086	T5083	dobj	neurons,sensitize
R2962	T5087	T5083	prep	to,sensitize
R2963	T5088	T5089	det	the,loss
R2964	T5089	T5087	pobj	loss,to
R2965	T5090	T5089	prep	of,loss
R2966	T5091	T5092	compound	battenin,activity
R2967	T5092	T5090	pobj	activity,of
R2968	T5093	T5083	punct	.,sensitize
R2969	T5095	T5096	advmod	Thus,provide
R297	T728	T725	punct	", ",decline
R2970	T5097	T5096	punct	", ",provide
R2971	T5098	T5099	poss	our,panel
R2972	T5099	T5096	nsubj	panel,provide
R2973	T5100	T5099	prep	of,panel
R2974	T5101	T5102	amod	wild,type
R2975	T5102	T5104	nmod	type,cells
R2976	T5103	T5102	punct	-,type
R2977	T5104	T5100	pobj	cells,of
R2978	T5105	T5102	punct	", ",type
R2979	T5106	T5102	conj	heterozygous,type
R298	T729	T725	cc	and,decline
R2980	T5107	T5106	punct	", ",heterozygous
R2981	T5108	T5106	cc	and,heterozygous
R2982	T5109	T5106	conj	homozygous,heterozygous
R2983	T5110	T5104	nmod	CbCln3Δex7,cells
R2984	T5111	T5110	punct	/,CbCln3Δex7
R2985	T5112	T5110	nummod	8,CbCln3Δex7
R2986	T5113	T5104	amod	cerebellar,cells
R2987	T5114	T5115	det	an,system
R2988	T5115	T5096	dobj	system,provide
R2989	T5116	T5115	amod	ideal,system
R299	T730	T731	amod	early,death
R2990	T5117	T5115	compound	model,system
R2991	T5118	T5119	aux	to,elucidate
R2992	T5119	T5115	advcl	elucidate,system
R2993	T5120	T5119	advmod	further,elucidate
R2994	T5121	T5122	compound	battenin,function
R2995	T5122	T5119	dobj	function,elucidate
R2996	T5123	T5122	cc	and,function
R2997	T5124	T5125	compound	JNCL,pathogenesis
R2998	T5125	T5122	conj	pathogenesis,function
R2999	T5126	T5096	punct	.,provide
R3	T170	T171	compound	Membrane,trafficking
R30	T205	T206	advmod	most,commonly
R300	T731	T725	conj	death,decline
R3000	T5236	T5237	nmod	Antibody,reagents
R3001	T5238	T5236	cc	and,Antibody
R3002	T5239	T5240	compound	cell,staining
R3003	T5240	T5236	conj	staining,Antibody
R3004	T5242	T5243	nmod	Nestin,antibodies
R3005	T5243	T5282	nsubjpass	antibodies,obtained
R3006	T5244	T5245	punct	(,μg
R3007	T5245	T5242	parataxis	μg,Nestin
R3008	T5246	T5247	compound	clone,Rat
R3009	T5247	T5245	dep	Rat,μg
R301	T732	T733	punct	[,1
R3010	T5248	T5247	nummod	401,Rat
R3011	T5249	T5245	punct	", ",μg
R3012	T5250	T5245	nummod	2,μg
R3013	T5251	T5252	punct	/,ml
R3014	T5252	T5245	prep	ml,μg
R3015	T5253	T5245	punct	),μg
R3016	T5254	T5242	punct	", ",Nestin
R3017	T5255	T5242	conj	Lamp,Nestin
R3018	T5256	T5255	nummod	1,Lamp
R3019	T5257	T5258	punct	(,μg
R302	T733	T717	parataxis	1,characterized
R3020	T5258	T5255	parataxis	μg,Lamp
R3021	T5259	T5260	compound	clone,1D4B
R3022	T5260	T5258	dep	1D4B,μg
R3023	T5261	T5258	punct	", ",μg
R3024	T5262	T5258	nummod	6,μg
R3025	T5263	T5264	punct	/,ml
R3026	T5264	T5258	prep	ml,μg
R3027	T5265	T5258	punct	),μg
R3028	T5266	T5255	punct	", ",Lamp
R3029	T5267	T5255	cc	and,Lamp
R303	T734	T733	punct	],1
R3030	T5268	T5255	conj	Lamp,Lamp
R3031	T5269	T5268	nummod	2,Lamp
R3032	T5270	T5271	punct	(,μg
R3033	T5271	T5268	parataxis	μg,Lamp
R3034	T5272	T5273	compound	clone,Abl
R3035	T5273	T5271	dep	Abl,μg
R3036	T5274	T5273	punct	-,Abl
R3037	T5275	T5273	nummod	93,Abl
R3038	T5276	T5271	punct	;,μg
R3039	T5277	T5271	nummod	6,μg
R304	T735	T700	punct	.,is
R3040	T5278	T5279	punct	/,ml
R3041	T5279	T5271	prep	ml,μg
R3042	T5280	T5271	punct	),μg
R3043	T5281	T5243	amod	monoclonal,antibodies
R3044	T5283	T5282	auxpass	were,obtained
R3045	T5284	T5282	prep	from,obtained
R3046	T5285	T5286	det	the,Bank
R3047	T5286	T5284	pobj	Bank,from
R3048	T5287	T5286	compound	Developmental,Bank
R3049	T5288	T5286	compound	Studies,Bank
R305	T737	T738	det	The,defect
R3050	T5289	T5286	compound	Hybridoma,Bank
R3051	T5290	T5286	punct	", ",Bank
R3052	T5291	T5286	acl	maintained,Bank
R3053	T5292	T5291	agent	by,maintained
R3054	T5293	T5294	det	The,University
R3055	T5294	T5295	nmod	University,Department
R3056	T5295	T5292	pobj	Department,by
R3057	T5296	T5294	prep	of,University
R3058	T5297	T5296	pobj	Iowa,of
R3059	T5298	T5295	punct	", ",Department
R306	T738	T741	nsubj	defect,is
R3060	T5299	T5295	prep	of,Department
R3061	T5300	T5301	compound	Biological,Sciences
R3062	T5301	T5299	pobj	Sciences,of
R3063	T5302	T5282	punct	.,obtained
R3064	T5304	T5305	nsubjpass	Batp1,described
R3065	T5306	T5307	punct	(,μg
R3066	T5307	T5304	parataxis	μg,Batp1
R3067	T5308	T5307	nummod	1,μg
R3068	T5309	T5310	punct	/,ml
R3069	T5310	T5307	prep	ml,μg
R307	T739	T738	amod	primary,defect
R3070	T5311	T5307	punct	),μg
R3071	T5312	T5305	auxpass	was,described
R3072	T5313	T5305	advmod	previously,described
R3073	T5314	T5315	punct	[,12
R3074	T5315	T5305	parataxis	12,described
R3075	T5316	T5315	punct	],12
R3076	T5317	T5305	punct	.,described
R3077	T5319	T5320	amod	Anti-subunit,c
R3078	T5320	T5321	amod	c,antibody
R3079	T5321	T5322	nsubjpass	antibody,provided
R308	T740	T738	amod	genetic,defect
R3080	T5323	T5324	punct	(,μg
R3081	T5324	T5321	parataxis	μg,antibody
R3082	T5325	T5324	nummod	0.7,μg
R3083	T5326	T5327	punct	/,ml
R3084	T5327	T5324	prep	ml,μg
R3085	T5328	T5324	punct	),μg
R3086	T5329	T5322	auxpass	was,provided
R3087	T5330	T5322	advmod	kindly,provided
R3088	T5331	T5322	agent	by,provided
R3089	T5332	T5333	compound	Dr.,Kominami
R309	T742	T743	punct	(,chromosomes
R3090	T5333	T5331	pobj	Kominami,by
R3091	T5334	T5335	punct	(,University
R3092	T5335	T5333	parataxis	University,Kominami
R3093	T5336	T5335	compound	Juntendo,University
R3094	T5337	T5335	punct	", ",University
R3095	T5338	T5335	npadvmod	Tokyo,University
R3096	T5339	T5335	punct	", ",University
R3097	T5340	T5335	npadvmod	Japan,University
R3098	T5341	T5335	punct	),University
R3099	T5342	T5322	punct	.,provided
R31	T206	T208	advmod	commonly,caused
R310	T743	T738	parataxis	chromosomes,defect
R3100	T5344	T5345	amod	Additional,antibodies
R3101	T5345	T5346	nsubj	antibodies,were
R3102	T5347	T5348	mark	as,follows
R3103	T5348	T5346	advcl	follows,were
R3104	T5349	T5346	punct	: ,were
R3105	T5350	T5346	dep	GFAP,were
R3106	T5351	T5350	punct	", ",GFAP
R3107	T5352	T5350	npadvmod	1,GFAP
R3108	T5353	T5354	punct	:,2000
R3109	T5354	T5352	prep	2000,1
R311	T744	T745	punct	>,80
R3110	T5355	T5356	punct	(,Corporation
R3111	T5356	T5350	parataxis	Corporation,GFAP
R3112	T5357	T5356	compound	DAKO,Corporation
R3113	T5358	T5356	punct	),Corporation
R3114	T5359	T5350	punct	;,GFAP
R3115	T5360	T5350	appos	calbindin,GFAP
R3116	T5361	T5360	punct	", ",calbindin
R3117	T5362	T5360	npadvmod	1,calbindin
R3118	T5363	T5364	punct	:,5000
R3119	T5364	T5362	prep	5000,1
R312	T745	T746	nummod	80,%
R3120	T5365	T5366	punct	(,Sigma
R3121	T5366	T5360	parataxis	Sigma,calbindin
R3122	T5367	T5366	punct	),Sigma
R3123	T5368	T5350	punct	;,GFAP
R3124	T5369	T5350	appos	NeuN,GFAP
R3125	T5370	T5369	punct	", ",NeuN
R3126	T5371	T5372	nummod	10,μg
R3127	T5372	T5369	npadvmod	μg,NeuN
R3128	T5373	T5374	punct	/,ml
R3129	T5374	T5372	prep	ml,μg
R313	T746	T743	compound	%,chromosomes
R3130	T5375	T5376	punct	(,Chemicon
R3131	T5376	T5369	parataxis	Chemicon,NeuN
R3132	T5377	T5376	punct	),Chemicon
R3133	T5378	T5350	punct	;,GFAP
R3134	T5379	T5380	compound	SV40,antigen
R3135	T5380	T5350	appos	antigen,GFAP
R3136	T5381	T5380	compound	T,antigen
R3137	T5382	T5380	punct	(,antigen
R3138	T5383	T5380	appos	Pab,antigen
R3139	T5384	T5383	nummod	101,Pab
R314	T747	T743	compound	disease,chromosomes
R3140	T5385	T5380	punct	),antigen
R3141	T5386	T5380	punct	", ",antigen
R3142	T5387	T5388	nummod	2,μg
R3143	T5388	T5380	npadvmod	μg,antigen
R3144	T5389	T5390	punct	/,ml
R3145	T5390	T5388	prep	ml,μg
R3146	T5391	T5392	punct	(,Biotechnology
R3147	T5392	T5380	parataxis	Biotechnology,antigen
R3148	T5393	T5392	compound	Santa,Biotechnology
R3149	T5394	T5392	compound	Cruz,Biotechnology
R315	T748	T743	punct	),chromosomes
R3150	T5395	T5392	punct	),Biotechnology
R3151	T5396	T5350	punct	;,GFAP
R3152	T5397	T5398	compound	cathepsin,D
R3153	T5398	T5350	appos	D,GFAP
R3154	T5399	T5398	punct	(,D
R3155	T5400	T5398	appos	C,D
R3156	T5401	T5400	punct	-,C
R3157	T5402	T5400	nummod	20,C
R3158	T5403	T5398	punct	),D
R3159	T5404	T5398	punct	", ",D
R316	T749	T738	acl	leading,defect
R3160	T5405	T5406	nummod	2,μg
R3161	T5406	T5398	npadvmod	μg,D
R3162	T5407	T5408	punct	/,ml
R3163	T5408	T5406	prep	ml,μg
R3164	T5409	T5410	punct	(,Biotechnology
R3165	T5410	T5398	parataxis	Biotechnology,D
R3166	T5411	T5410	compound	Santa,Biotechnology
R3167	T5412	T5410	compound	Cruz,Biotechnology
R3168	T5413	T5410	punct	),Biotechnology
R3169	T5414	T5350	punct	;,GFAP
R317	T750	T749	prep	to,leading
R3170	T5415	T5416	compound	cytochrome,subunit
R3171	T5416	T5350	appos	subunit,GFAP
R3172	T5417	T5416	compound	c,subunit
R3173	T5418	T5416	compound	oxidase,subunit
R3174	T5419	T5416	nummod	IV,subunit
R3175	T5420	T5416	punct	(,subunit
R3176	T5421	T5416	appos	cox4,subunit
R3177	T5422	T5416	punct	),subunit
R3178	T5423	T5416	punct	", ",subunit
R3179	T5424	T5416	npadvmod	1,subunit
R318	T751	T750	pobj	JNCL,to
R3180	T5425	T5426	punct	:,1000
R3181	T5426	T5424	prep	1000,1
R3182	T5427	T5428	punct	(,Clontech
R3183	T5428	T5416	parataxis	Clontech,subunit
R3184	T5429	T5428	compound	BD,Clontech
R3185	T5430	T5428	compound	Biosciences,Clontech
R3186	T5431	T5428	punct	),Clontech
R3187	T5432	T5350	punct	;,GFAP
R3188	T5433	T5350	appos	PDI,GFAP
R3189	T5434	T5433	punct	(,PDI
R319	T752	T753	det	a,deletion
R3190	T5435	T5433	appos	H,PDI
R3191	T5436	T5435	punct	-,H
R3192	T5437	T5435	nummod	160,H
R3193	T5438	T5433	punct	),PDI
R3194	T5439	T5433	punct	", ",PDI
R3195	T5440	T5441	nummod	2,μg
R3196	T5441	T5433	npadvmod	μg,PDI
R3197	T5442	T5443	punct	/,ml
R3198	T5443	T5441	prep	ml,μg
R3199	T5444	T5445	punct	(,Biotechnology
R32	T207	T206	punct	-,commonly
R320	T753	T741	attr	deletion,is
R3200	T5445	T5433	parataxis	Biotechnology,PDI
R3201	T5446	T5445	compound	Santa,Biotechnology
R3202	T5447	T5445	compound	Cruz,Biotechnology
R3203	T5448	T5445	punct	),Biotechnology
R3204	T5449	T5350	punct	;,GFAP
R3205	T5450	T5350	appos	GM130,GFAP
R3206	T5451	T5450	punct	", ",GM130
R3207	T5452	T5453	nummod	1,μg
R3208	T5453	T5450	npadvmod	μg,GM130
R3209	T5454	T5455	punct	/,ml
R321	T754	T755	nummod	1.02,kb
R3210	T5455	T5453	prep	ml,μg
R3211	T5456	T5457	punct	(,Labs
R3212	T5457	T5450	parataxis	Labs,GM130
R3213	T5458	T5457	compound	BD,Labs
R3214	T5459	T5457	compound	Transduction,Labs
R3215	T5460	T5457	punct	),Labs
R3216	T5461	T5350	punct	;,GFAP
R3217	T5462	T5463	compound	α,tubulin
R3218	T5463	T5350	appos	tubulin,GFAP
R3219	T5464	T5463	punct	-,tubulin
R322	T755	T753	nmod	kb,deletion
R3220	T5465	T5463	punct	", ",tubulin
R3221	T5466	T5463	npadvmod	1,tubulin
R3222	T5467	T5468	punct	:,"15,000"
R3223	T5468	T5466	prep	"15,000",1
R3224	T5469	T5470	punct	(,Sigma
R3225	T5470	T5463	parataxis	Sigma,tubulin
R3226	T5471	T5470	punct	),Sigma
R3227	T5472	T5350	punct	;,GFAP
R3228	T5473	T5350	appos	grp75,GFAP
R3229	T5474	T5473	punct	", ",grp75
R323	T756	T753	amod	genomic,deletion
R3230	T5475	T5473	npadvmod	1,grp75
R3231	T5476	T5477	punct	:,200
R3232	T5477	T5475	prep	200,1
R3233	T5478	T5479	punct	(,Stressgen
R3234	T5479	T5473	parataxis	Stressgen,grp75
R3235	T5480	T5479	punct	),Stressgen
R3236	T5481	T5350	punct	;,GFAP
R3237	T5482	T5483	amod	early,antigen
R3238	T5483	T5350	appos	antigen,GFAP
R3239	T5484	T5483	compound	endosome,antigen
R324	T757	T753	compound	DNA,deletion
R3240	T5485	T5483	punct	-,antigen
R3241	T5486	T5483	nummod	1,antigen
R3242	T5487	T5483	punct	(,antigen
R3243	T5488	T5483	appos	EEA1,antigen
R3244	T5489	T5483	punct	),antigen
R3245	T5490	T5483	punct	", ",antigen
R3246	T5491	T5492	nummod	2,μg
R3247	T5492	T5483	npadvmod	μg,antigen
R3248	T5493	T5494	punct	/,ml
R3249	T5494	T5492	prep	ml,μg
R325	T758	T753	prep	in,deletion
R3250	T5495	T5496	punct	(,Biotechnology
R3251	T5496	T5483	parataxis	Biotechnology,antigen
R3252	T5497	T5496	compound	Santa,Biotechnology
R3253	T5498	T5496	compound	Cruz,Biotechnology
R3254	T5499	T5496	punct	),Biotechnology
R3255	T5500	T5350	punct	;,GFAP
R3256	T5501	T5350	appos	rab,GFAP
R3257	T5502	T5501	nummod	7,rab
R3258	T5503	T5501	punct	", ",rab
R3259	T5504	T5505	nummod	4,μg
R326	T759	T760	det	the,gene
R3260	T5505	T5501	npadvmod	μg,rab
R3261	T5506	T5507	punct	/,ml
R3262	T5507	T5505	prep	ml,μg
R3263	T5508	T5509	punct	(,Biotechnology
R3264	T5509	T5501	parataxis	Biotechnology,rab
R3265	T5510	T5509	compound	Santa,Biotechnology
R3266	T5511	T5509	compound	Cruz,Biotechnology
R3267	T5512	T5509	punct	),Biotechnology
R3268	T5513	T5346	punct	.,were
R3269	T5515	T5516	det	All,antibodies
R327	T760	T758	pobj	gene,in
R3270	T5516	T5519	nsubjpass	antibodies,obtained
R3271	T5517	T5516	amod	fluorescent,antibodies
R3272	T5518	T5516	amod	secondary,antibodies
R3273	T5520	T5519	auxpass	were,obtained
R3274	T5521	T5519	prep	from,obtained
R3275	T5522	T5523	compound	Jackson,Laboratories
R3276	T5523	T5521	pobj	Laboratories,from
R3277	T5524	T5523	compound	ImmunoResearch,Laboratories
R3278	T5525	T5519	cc	and,obtained
R3279	T5526	T5527	npadvmod	HRP,conjugated
R328	T761	T760	compound	CLN3,gene
R3280	T5527	T5529	amod	conjugated,antibodies
R3281	T5528	T5527	punct	-,conjugated
R3282	T5529	T5531	nsubjpass	antibodies,obtained
R3283	T5530	T5529	amod	secondary,antibodies
R3284	T5531	T5519	conj	obtained,obtained
R3285	T5532	T5531	auxpass	were,obtained
R3286	T5533	T5531	prep	from,obtained
R3287	T5534	T5535	compound	Amersham,Biosciences
R3288	T5535	T5533	pobj	Biosciences,from
R3289	T5536	T5531	punct	.,obtained
R329	T762	T753	punct	", ",deletion
R3290	T5538	T5539	amod	Additional,markers
R3291	T5539	T5541	nsubj	markers,were
R3292	T5540	T5539	compound	cell,markers
R3293	T5542	T5543	mark	as,follows
R3294	T5543	T5541	advcl	follows,were
R3295	T5544	T5541	punct	: ,were
R3296	T5545	T5546	compound	VVL,biotin
R3297	T5546	T5541	dep	biotin,were
R3298	T5547	T5546	punct	-,biotin
R3299	T5548	T5546	punct	", ",biotin
R33	T208	T197	acl	caused,disease
R330	T763	T764	dep	which,eliminates
R3300	T5549	T5546	npadvmod	1,biotin
R3301	T5550	T5551	punct	:,2000
R3302	T5551	T5549	prep	2000,1
R3303	T5552	T5553	punct	(,Laboratories
R3304	T5553	T5546	parataxis	Laboratories,biotin
R3305	T5554	T5553	compound	Vector,Laboratories
R3306	T5555	T5553	punct	),Laboratories
R3307	T5556	T5546	punct	", ",biotin
R3308	T5557	T5558	nummod	"10,000",MW
R3309	T5558	T5559	compound	MW,FITC
R331	T764	T753	relcl	eliminates,deletion
R3310	T5559	T5546	conj	FITC,biotin
R3311	T5560	T5559	compound	dextran,FITC
R3312	T5561	T5559	punct	-,FITC
R3313	T5562	T5559	punct	", ",FITC
R3314	T5563	T5564	nummod	1,mg
R3315	T5564	T5559	npadvmod	mg,FITC
R3316	T5565	T5566	punct	/,ml
R3317	T5566	T5564	prep	ml,mg
R3318	T5567	T5559	cc	and,FITC
R3319	T5568	T5569	compound	Lysotracker,DND
R332	T765	T766	nmod	exons,7
R3320	T5569	T5559	conj	DND,FITC
R3321	T5570	T5569	compound	Red,DND
R3322	T5571	T5569	punct	-,DND
R3323	T5572	T5569	nummod	99,DND
R3324	T5573	T5569	punct	", ",DND
R3325	T5574	T5575	nummod	500,nM
R3326	T5575	T5569	npadvmod	nM,DND
R3327	T5576	T5577	punct	(,Probes
R3328	T5577	T5569	parataxis	Probes,DND
R3329	T5578	T5577	compound	Molecular,Probes
R333	T766	T764	dobj	7,eliminates
R3330	T5579	T5577	punct	),Probes
R3331	T5580	T5541	punct	.,were
R3332	T5714	T5713	punct	", ",Generation
R3333	T5715	T5713	conj	maintenance,Generation
R3334	T5716	T5715	cc	and,maintenance
R3335	T5717	T5715	conj	differentiation,maintenance
R3336	T5718	T5713	prep	of,Generation
R3337	T5719	T5720	nmod	CbCln3,lines
R3338	T5720	T5718	pobj	lines,of
R3339	T5721	T5720	amod	cerebellar,lines
R334	T767	T766	cc	and,7
R3340	T5722	T5723	amod	neuronal,precursor
R3341	T5723	T5720	compound	precursor,lines
R3342	T5725	T5726	nmod	Cln3Δex7,mice
R3343	T5726	T5732	nsubjpass	mice,described
R3344	T5727	T5725	punct	/,Cln3Δex7
R3345	T5728	T5725	nummod	8,Cln3Δex7
R3346	T5729	T5726	amod	knock,mice
R3347	T5730	T5729	punct	-,knock
R3348	T5731	T5729	prt	in,knock
R3349	T5733	T5732	aux	have,described
R335	T768	T766	conj	8,7
R3350	T5734	T5732	auxpass	been,described
R3351	T5735	T5732	advmod	previously,described
R3352	T5736	T5737	punct	[,12
R3353	T5737	T5732	parataxis	12,described
R3354	T5738	T5737	punct	],12
R3355	T5739	T5732	punct	.,described
R3356	T5741	T5742	compound	Littermate,pups
R3357	T5742	T5743	nsubjpass	pups,used
R3358	T5744	T5742	prep	from,pups
R3359	T5745	T5746	compound	heterozygote,crosses
R336	T769	T766	cc	and,7
R3360	T5746	T5744	pobj	crosses,from
R3361	T5747	T5743	auxpass	were,used
R3362	T5748	T5743	prep	for,used
R3363	T5749	T5750	amod	primary,establishment
R3364	T5750	T5748	pobj	establishment,for
R3365	T5751	T5750	compound	culture,establishment
R3366	T5752	T5743	punct	", ",used
R3367	T5753	T5743	prep	by,used
R3368	T5754	T5755	advmod	previously,described
R3369	T5755	T5756	amod	described,methods
R337	T770	T771	amod	surrounding,DNA
R3370	T5756	T5753	pobj	methods,by
R3371	T5757	T5758	dep	that,yield
R3372	T5758	T5756	relcl	yield,methods
R3373	T5759	T5760	amod	cerebellar,neuron
R3374	T5760	T5762	npadvmod	neuron,enriched
R3375	T5761	T5760	compound	granule,neuron
R3376	T5762	T5764	amod	enriched,cultures
R3377	T5763	T5762	punct	-,enriched
R3378	T5764	T5758	dobj	cultures,yield
R3379	T5765	T5766	punct	[,26
R338	T771	T766	conj	DNA,7
R3380	T5766	T5758	parataxis	26,yield
R3381	T5767	T5766	punct	],26
R3382	T5768	T5743	punct	.,used
R3383	T5770	T5771	amod	Postnatal,day
R3384	T5771	T5772	nmod	day,cerebella
R3385	T5772	T5777	nsubjpass	cerebella,dissected
R3386	T5773	T5771	nummod	4,day
R3387	T5774	T5771	punct	(,day
R3388	T5775	T5771	appos	P4,day
R3389	T5776	T5772	punct	),cerebella
R339	T772	T771	amod	intronic,DNA
R3390	T5778	T5777	auxpass	were,dissected
R3391	T5779	T5777	prep	in,dissected
R3392	T5780	T5781	poss	Hank,Solution
R3393	T5781	T5779	pobj	Solution,in
R3394	T5782	T5780	case	's,Hank
R3395	T5783	T5781	compound	Balanced,Solution
R3396	T5784	T5781	compound	Salt,Solution
R3397	T5785	T5781	punct	(,Solution
R3398	T5786	T5781	appos	HBSS,Solution
R3399	T5787	T5788	punct	", ",Sigma
R34	T209	T208	agent	by,caused
R340	T773	T741	punct	", ",is
R3400	T5788	T5781	parataxis	Sigma,Solution
R3401	T5789	T5788	punct	),Sigma
R3402	T5790	T5781	punct	", ",Solution
R3403	T5791	T5781	acl	supplemented,Solution
R3404	T5792	T5791	prep	with,supplemented
R3405	T5793	T5794	nummod	35,mM
R3406	T5794	T5795	compound	mM,glucose
R3407	T5795	T5792	pobj	glucose,with
R3408	T5796	T5777	punct	.,dissected
R3409	T5798	T5799	compound	Tail,biopsies
R341	T774	T741	advcl	predicting,is
R3410	T5799	T5800	nsubjpass	biopsies,collected
R3411	T5801	T5800	auxpass	were,collected
R3412	T5802	T5800	advmod	also,collected
R3413	T5803	T5800	prep	for,collected
R3414	T5804	T5805	amod	genomic,isolation
R3415	T5805	T5803	pobj	isolation,for
R3416	T5806	T5805	compound	DNA,isolation
R3417	T5807	T5805	cc	and,isolation
R3418	T5808	T5809	amod	genotypic,analysis
R3419	T5809	T5805	conj	analysis,isolation
R342	T775	T776	det	a,product
R3420	T5810	T5800	punct	.,collected
R3421	T5812	T5813	compound	Trypsin,EDTA
R3422	T5813	T5815	nsubj	EDTA,helped
R3423	T5814	T5813	punct	/,EDTA
R3424	T5816	T5817	punct	(,Sigma
R3425	T5817	T5813	parataxis	Sigma,EDTA
R3426	T5818	T5819	nummod	10,mg
R3427	T5819	T5817	dep	mg,Sigma
R3428	T5820	T5821	punct	/,ml
R3429	T5821	T5819	prep	ml,mg
R343	T776	T774	dobj	product,predicting
R3430	T5822	T5817	punct	", ",Sigma
R3431	T5823	T5817	punct	),Sigma
R3432	T5824	T5813	cc	and,EDTA
R3433	T5825	T5813	conj	DNase,EDTA
R3434	T5826	T5825	nummod	I,DNase
R3435	T5827	T5828	punct	(,Sigma
R3436	T5828	T5825	parataxis	Sigma,DNase
R3437	T5829	T5830	nummod	100,μg
R3438	T5830	T5828	dep	μg,Sigma
R3439	T5831	T5832	punct	/,ml
R344	T777	T776	amod	non-functional,product
R3440	T5832	T5830	prep	ml,μg
R3441	T5833	T5828	punct	", ",Sigma
R3442	T5834	T5828	punct	),Sigma
R3443	T5835	T5813	punct	", ",EDTA
R3444	T5836	T5813	acl	suspended,EDTA
R3445	T5837	T5836	prep	in,suspended
R3446	T5838	T5837	pobj	HBSS,in
R3447	T5839	T5815	punct	", ",helped
R3448	T5840	T5815	xcomp	dissociate,helped
R3449	T5841	T5840	dobj	cerebella,dissociate
R345	T778	T776	compound	protein,product
R3450	T5842	T5840	prep	for,dissociate
R3451	T5843	T5844	amod	primary,culture
R3452	T5844	T5845	compound	culture,plating
R3453	T5845	T5842	pobj	plating,for
R3454	T5846	T5845	prep	onto,plating
R3455	T5847	T5848	nummod	0.01,%
R3456	T5848	T5849	compound	%,poly-ornithine
R3457	T5849	T5850	npadvmod	poly-ornithine,coated
R3458	T5850	T5854	amod	coated,dishes
R3459	T5851	T5852	punct	(,Sigma
R346	T779	T780	punct	[,2
R3460	T5852	T5849	parataxis	Sigma,poly-ornithine
R3461	T5853	T5852	punct	),Sigma
R3462	T5854	T5846	pobj	dishes,onto
R3463	T5855	T5856	nummod	100,mm
R3464	T5856	T5854	compound	mm,dishes
R3465	T5857	T5815	punct	.,helped
R3466	T5859	T5860	amod	Primary,cultures
R3467	T5860	T5861	nsubjpass	cultures,cultured
R3468	T5862	T5860	prep	from,cultures
R3469	T5863	T5864	amod	individual,cerebella
R347	T780	T741	parataxis	2,is
R3470	T5864	T5862	pobj	cerebella,from
R3471	T5865	T5861	auxpass	were,cultured
R3472	T5866	T5861	advmod	overnight,cultured
R3473	T5867	T5861	prep	at,cultured
R3474	T5868	T5869	nummod	37,°C
R3475	T5869	T5867	pobj	°C,at
R3476	T5870	T5869	punct	", ",°C
R3477	T5871	T5872	nummod	5,%
R3478	T5872	T5873	compound	%,CO2
R3479	T5873	T5869	appos	CO2,°C
R348	T781	T780	punct	],2
R3480	T5874	T5861	punct	", ",cultured
R3481	T5875	T5861	prep	in,cultured
R3482	T5876	T5877	compound	granule,neuron
R3483	T5877	T5878	compound	neuron,media
R3484	T5878	T5875	pobj	media,in
R3485	T5879	T5878	compound	growth,media
R3486	T5880	T5878	punct	(,media
R3487	T5881	T5882	poss	Dulbecco,Medium
R3488	T5882	T5878	appos	Medium,media
R3489	T5883	T5881	case	's,Dulbecco
R349	T782	T741	punct	.,is
R3490	T5884	T5882	compound	Modified,Medium
R3491	T5885	T5882	compound	Eagle,Medium
R3492	T5886	T5882	punct	[,Medium
R3493	T5887	T5882	appos	DMEM,Medium
R3494	T5888	T5882	punct	", ",Medium
R3495	T5889	T5890	compound	Gibco,#
R3496	T5890	T5882	appos	#,Medium
R3497	T5891	T5890	compound	BRL,#
R3498	T5892	T5890	nummod	11995,#
R3499	T5893	T5890	punct	-,#
R35	T210	T211	det	a,mutation
R350	T784	T785	det	The,hallmark
R3500	T5894	T5890	nummod	065,#
R3501	T5895	T5882	punct	],Medium
R3502	T5896	T5882	punct	", ",Medium
R3503	T5897	T5898	nummod	10,%
R3504	T5898	T5882	appos	%,Medium
R3505	T5899	T5900	amod	fetal,serum
R3506	T5900	T5898	appos	serum,%
R3507	T5901	T5900	amod	bovine,serum
R3508	T5902	T5900	punct	[,serum
R3509	T5903	T5904	compound	Sigma,F
R351	T785	T787	nsubj	hallmark,is
R3510	T5904	T5900	appos	F,serum
R3511	T5905	T5904	compound	#,F
R3512	T5906	T5904	punct	-,F
R3513	T5907	T5904	nummod	2442,F
R3514	T5908	T5882	punct	],Medium
R3515	T5909	T5882	punct	", ",Medium
R3516	T5910	T5882	acl	supplemented,Medium
R3517	T5911	T5910	prep	with,supplemented
R3518	T5912	T5913	nummod	24,mM
R3519	T5913	T5914	compound	mM,KCl
R352	T786	T785	amod	pathological,hallmark
R3520	T5914	T5911	pobj	KCl,with
R3521	T5915	T5861	punct	),cultured
R3522	T5916	T5861	punct	.,cultured
R3523	T5918	T5919	nsubjpass	Infection,performed
R3524	T5920	T5919	auxpass	was,performed
R3525	T5921	T5922	det	the,day
R3526	T5922	T5919	npadvmod	day,performed
R3527	T5923	T5922	amod	following,day
R3528	T5924	T5919	prep	with,performed
R3529	T5925	T5926	amod	defective,retrovirus
R353	T788	T785	prep	of,hallmark
R3530	T5926	T5927	nsubj	retrovirus,transducing
R3531	T5927	T5924	pcomp	transducing,with
R3532	T5928	T5929	det	the,antigen
R3533	T5929	T5927	dobj	antigen,transducing
R3534	T5930	T5931	npadvmod	temperature,sensitive
R3535	T5931	T5929	amod	sensitive,antigen
R3536	T5932	T5931	punct	-,sensitive
R3537	T5933	T5934	nmod	tsA58,U19
R3538	T5934	T5929	nmod	U19,antigen
R3539	T5935	T5934	punct	/,U19
R354	T789	T788	pobj	JNCL,of
R3540	T5936	T5929	amod	large,antigen
R3541	T5937	T5929	compound	T,antigen
R3542	T5938	T5929	cc	and,antigen
R3543	T5939	T5940	det	a,cassette
R3544	T5940	T5929	conj	cassette,antigen
R3545	T5941	T5940	compound	selection,cassette
R3546	T5942	T5943	compound	neomycin,resistance
R3547	T5943	T5940	compound	resistance,cassette
R3548	T5944	T5943	punct	-,resistance
R3549	T5945	T5946	punct	[,27
R355	T790	T791	amod	autofluorescent,deposits
R3550	T5946	T5940	parataxis	27,cassette
R3551	T5947	T5946	punct	],27
R3552	T5948	T5927	punct	", ",transducing
R3553	T5949	T5950	mark	as,described
R3554	T5950	T5927	advcl	described,transducing
R3555	T5951	T5950	advmod	previously,described
R3556	T5952	T5953	punct	[,28
R3557	T5953	T5950	parataxis	28,described
R3558	T5954	T5953	punct	],28
R3559	T5955	T5919	punct	.,performed
R356	T791	T787	attr	deposits,is
R3560	T5957	T5958	prep	Following,shifted
R3561	T5959	T5957	pobj	infection,Following
R3562	T5960	T5958	punct	", ",shifted
R3563	T5961	T5958	nsubjpass	cultures,shifted
R3564	T5962	T5958	auxpass	were,shifted
R3565	T5963	T5958	prep	to,shifted
R3566	T5964	T5965	det	the,temperature
R3567	T5965	T5963	pobj	temperature,to
R3568	T5966	T5967	compound	tsA58,U19
R3569	T5967	T5969	npadvmod	U19,permissive
R357	T792	T791	compound	ceroid,deposits
R3570	T5968	T5967	punct	/,U19
R3571	T5969	T5965	amod	permissive,temperature
R3572	T5970	T5965	compound	growth,temperature
R3573	T5971	T5965	prep	of,temperature
R3574	T5972	T5973	nummod	33,°C
R3575	T5973	T5971	pobj	°C,of
R3576	T5974	T5958	cc	and,shifted
R3577	T5975	T5976	nsubj	selection,was
R3578	T5976	T5958	conj	was,shifted
R3579	T5977	T5976	prep	in,was
R358	T793	T791	compound	lipofuscin,deposits
R3580	T5978	T5979	det	the,media
R3581	T5979	T5977	pobj	media,in
R3582	T5980	T5979	amod	same,media
R3583	T5981	T5979	compound	growth,media
R3584	T5982	T5979	prep	as,media
R3585	T5983	T5982	pcomp	above,as
R3586	T5984	T5976	punct	", ",was
R3587	T5985	T5976	prep	with,was
R3588	T5986	T5987	nummod	200,μg
R3589	T5987	T5988	nmod	μg,G418
R359	T794	T791	prep	within,deposits
R3590	T5988	T5985	pobj	G418,with
R3591	T5989	T5990	punct	/,ml
R3592	T5990	T5987	prep	ml,μg
R3593	T5991	T5976	punct	.,was
R3594	T5993	T5994	advmod	Conditionally,immortalized
R3595	T5994	T5995	amod	immortalized,colonies
R3596	T5995	T5996	nsubjpass	colonies,isolated
R3597	T5997	T5996	auxpass	were,isolated
R3598	T5998	T5999	quantmod	3,9
R3599	T5999	T6001	nummod	9,weeks
R36	T211	T209	pobj	mutation,by
R360	T795	T794	pobj	autolysosomes,within
R3600	T6000	T5999	punct	–,9
R3601	T6001	T6002	npadvmod	weeks,post-infection
R3602	T6002	T5996	advmod	post-infection,isolated
R3603	T6003	T6004	cc	and,expanded
R3604	T6004	T5996	dep	expanded,isolated
R3605	T6005	T6004	prep	for,expanded
R3606	T6006	T6007	amod	frozen,stocks
R3607	T6007	T6005	pobj	stocks,for
R3608	T6008	T6007	cc	and,stocks
R3609	T6009	T6010	amod	further,isolation
R361	T796	T797	dep	that,are
R3610	T6010	T6007	conj	isolation,stocks
R3611	T6011	T6010	compound	sub-clone,isolation
R3612	T6012	T5996	punct	.,isolated
R3613	T6014	T6015	amod	Multiple,lines
R3614	T6015	T6017	nsubjpass	lines,obtained
R3615	T6016	T6015	amod	clonal,lines
R3616	T6018	T6017	auxpass	were,obtained
R3617	T6019	T6017	prep	for,obtained
R3618	T6020	T6021	det	each,genotype
R3619	T6021	T6019	pobj	genotype,for
R362	T797	T791	relcl	are,deposits
R3620	T6022	T6021	cc	and,genotype
R3621	T6023	T6024	det	all,phenotypes
R3622	T6024	T6021	conj	phenotypes,genotype
R3623	T6025	T6026	auxpass	were,confirmed
R3624	T6026	T6017	dep	confirmed,obtained
R3625	T6027	T6026	prep	in,confirmed
R3626	T6028	T6029	advmod	at,2
R3627	T6029	T6031	nummod	2,lines
R3628	T6030	T6029	advmod	least,2
R3629	T6031	T6027	pobj	lines,in
R363	T798	T797	acomp	enriched,are
R3630	T6032	T6031	amod	independent,lines
R3631	T6033	T6031	compound	CbCln3,lines
R3632	T6034	T6031	compound	cell,lines
R3633	T6035	T6017	punct	.,obtained
R3634	T6037	T6038	compound	CbCln3,lines
R3635	T6038	T6040	nsubjpass	lines,maintained
R3636	T6039	T6038	compound	cell,lines
R3637	T6041	T6040	auxpass	were,maintained
R3638	T6042	T6040	prep	on,maintained
R3639	T6043	T6044	nummod	0.01,%
R364	T799	T797	prep	in,are
R3640	T6044	T6045	nmod	%,dishes
R3641	T6045	T6042	pobj	dishes,on
R3642	T6046	T6047	compound	poly,ornithine
R3643	T6047	T6049	npadvmod	ornithine,coated
R3644	T6048	T6047	punct	-,ornithine
R3645	T6049	T6045	amod	coated,dishes
R3646	T6050	T6040	prep	at,maintained
R3647	T6051	T6052	quantmod	30,90
R3648	T6052	T6054	nummod	90,%
R3649	T6053	T6052	punct	–,90
R365	T800	T801	compound	subunit,c
R3650	T6054	T6055	compound	%,confluency
R3651	T6055	T6050	pobj	confluency,at
R3652	T6056	T6040	punct	", ",maintained
R3653	T6057	T6040	prep	in,maintained
R3654	T6058	T6059	nummod	33,°C
R3655	T6059	T6057	pobj	°C,in
R3656	T6060	T6059	cc	and,°C
R3657	T6061	T6062	nummod	5,atmosphere
R3658	T6062	T6059	conj	atmosphere,°C
R3659	T6063	T6061	punct	%,5
R366	T801	T799	pobj	c,in
R3660	T6064	T6062	compound	CO2,atmosphere
R3661	T6065	T6040	punct	.,maintained
R3662	T6067	T6068	compound	Passage,number
R3663	T6068	T6069	nsubjpass	number,recorded
R3664	T6070	T6069	auxpass	was,recorded
R3665	T6071	T6072	punct	(,passages
R3666	T6072	T6069	parataxis	passages,recorded
R3667	T6073	T6074	quantmod	up,20
R3668	T6074	T6072	nummod	20,passages
R3669	T6075	T6074	quantmod	to,20
R367	T802	T801	prep	of,c
R3670	T6076	T6074	punct	~,20
R3671	T6077	T6072	punct	),passages
R3672	T6078	T6069	punct	", ",recorded
R3673	T6079	T6069	cc	but,recorded
R3674	T6080	T6069	conj	had,recorded
R3675	T6081	T6082	det	no,impact
R3676	T6082	T6080	dobj	impact,had
R3677	T6083	T6082	amod	apparent,impact
R3678	T6084	T6082	prep	on,impact
R3679	T6085	T6084	pobj	phenotype,on
R368	T803	T804	det	the,complex
R3680	T6086	T6069	punct	.,recorded
R3681	T6088	T6089	amod	Neuronal,differentiation
R3682	T6089	T6090	nsubj	differentiation,was
R3683	T6091	T6092	mark	as,described
R3684	T6092	T6090	advcl	described,was
R3685	T6093	T6092	advmod	previously,described
R3686	T6094	T6095	punct	[,29
R3687	T6095	T6092	parataxis	29,described
R3688	T6096	T6095	punct	],29
R3689	T6097	T6090	prep	with,was
R369	T804	T802	pobj	complex,of
R3690	T6098	T6099	det	the,cocktail
R3691	T6099	T6097	pobj	cocktail,with
R3692	T6100	T6099	amod	following,cocktail
R3693	T6101	T6099	punct	: ,cocktail
R3694	T6102	T6103	nummod	10,ng
R3695	T6103	T6104	nmod	ng,FGF
R3696	T6104	T6099	appos	FGF,cocktail
R3697	T6105	T6106	punct	/,ml
R3698	T6106	T6103	prep	ml,ng
R3699	T6107	T6104	compound	α,FGF
R37	T212	T213	punct	~,1
R370	T805	T806	amod	mitochondrial,synthase
R3700	T6108	T6104	punct	-,FGF
R3701	T6109	T6104	punct	", ",FGF
R3702	T6110	T6111	nummod	250,μM
R3703	T6111	T6112	compound	μM,IBMX
R3704	T6112	T6104	appos	IBMX,FGF
R3705	T6113	T6104	punct	", ",FGF
R3706	T6114	T6115	nummod	200,nM
R3707	T6115	T6116	compound	nM,TPA
R3708	T6116	T6104	appos	TPA,FGF
R3709	T6117	T6104	punct	", ",FGF
R371	T806	T804	compound	synthase,complex
R3710	T6118	T6119	nummod	50,μM
R3711	T6119	T6120	compound	μM,forskolin
R3712	T6120	T6104	appos	forskolin,FGF
R3713	T6121	T6104	punct	", ",FGF
R3714	T6122	T6123	nummod	5,μM
R3715	T6123	T6124	compound	μM,dopamine
R3716	T6124	T6104	appos	dopamine,FGF
R3717	T6125	T6126	punct	(,Sigma
R3718	T6126	T6104	parataxis	Sigma,FGF
R3719	T6127	T6126	punct	),Sigma
R372	T807	T806	compound	ATP,synthase
R3720	T6128	T6090	punct	.,was
R3721	T6166	T6165	cc	and,Genotyping
R3722	T6167	T6168	compound	RT,PCR
R3723	T6168	T6165	conj	PCR,Genotyping
R3724	T6169	T6168	punct	-,PCR
R3725	T6171	T6172	amod	Genomic,DNA
R3726	T6172	T6173	nsubjpass	DNA,extracted
R3727	T6174	T6173	auxpass	was,extracted
R3728	T6175	T6173	prep	from,extracted
R3729	T6176	T6177	compound	tail,biopsies
R373	T808	T809	punct	[,3
R3730	T6177	T6175	pobj	biopsies,from
R3731	T6178	T6177	cc	and,biopsies
R3732	T6179	T6180	compound	cell,pellets
R3733	T6180	T6177	conj	pellets,biopsies
R3734	T6181	T6182	mark	as,described
R3735	T6182	T6173	advcl	described,extracted
R3736	T6183	T6184	punct	(,Cotman
R3737	T6184	T6173	meta	Cotman,extracted
R3738	T6185	T6184	nmod	et,Cotman
R3739	T6186	T6184	nmod	al.,Cotman
R374	T809	T787	parataxis	3,is
R3740	T6187	T6184	punct	", ",Cotman
R3741	T6188	T6184	nummod	2002,Cotman
R3742	T6189	T6184	punct	),Cotman
R3743	T6190	T6173	punct	.,extracted
R3744	T6192	T6193	nmod	Cln3Δex7,allele
R3745	T6193	T6199	compound	allele,genotyping
R3746	T6194	T6192	punct	/,Cln3Δex7
R3747	T6195	T6192	nummod	8,Cln3Δex7
R3748	T6196	T6193	amod	knock,allele
R3749	T6197	T6196	punct	-,knock
R375	T810	T811	punct	-,5
R3750	T6198	T6196	prt	in,knock
R3751	T6199	T6201	nsubj	genotyping,was
R3752	T6200	T6199	compound	PCR,genotyping
R3753	T6202	T6201	prep	with,was
R3754	T6203	T6204	amod	wild,type
R3755	T6204	T6206	compound	type,primers
R3756	T6205	T6204	punct	-,type
R3757	T6206	T6202	pobj	primers,with
R3758	T6207	T6206	punct	", ",primers
R3759	T6208	T6206	appos	WtF,primers
R376	T811	T809	prep	5,3
R3760	T6209	T6208	punct	(,WtF
R3761	T6210	T6211	nummod	5,CAGCATCTCCTCAGGGCTA
R3762	T6211	T6208	appos	CAGCATCTCCTCAGGGCTA,WtF
R3763	T6212	T6210	punct	',5
R3764	T6213	T6211	punct	-,CAGCATCTCCTCAGGGCTA
R3765	T6214	T6211	punct	-,CAGCATCTCCTCAGGGCTA
R3766	T6215	T6211	nummod	3,CAGCATCTCCTCAGGGCTA
R3767	T6216	T6211	punct	',CAGCATCTCCTCAGGGCTA
R3768	T6217	T6208	punct	),WtF
R3769	T6218	T6208	cc	and,WtF
R377	T812	T809	punct	],3
R3770	T6219	T6208	conj	WtR,WtF
R3771	T6220	T6219	punct	(,WtR
R3772	T6221	T6222	nummod	5,CCAACATAGAAAGTAGGGTGTGC
R3773	T6222	T6219	appos	CCAACATAGAAAGTAGGGTGTGC,WtR
R3774	T6223	T6221	punct	',5
R3775	T6224	T6222	punct	-,CCAACATAGAAAGTAGGGTGTGC
R3776	T6225	T6222	punct	-,CCAACATAGAAAGTAGGGTGTGC
R3777	T6226	T6222	nummod	3,CCAACATAGAAAGTAGGGTGTGC
R3778	T6227	T6222	punct	',CCAACATAGAAAGTAGGGTGTGC
R3779	T6228	T6201	punct	),was
R378	T813	T787	punct	.,is
R3780	T6229	T6230	aux	to,yield
R3781	T6230	T6201	advcl	yield,was
R3782	T6231	T6232	det	a,band
R3783	T6232	T6230	dobj	band,yield
R3784	T6233	T6234	punct	~,250
R3785	T6234	T6235	nummod	250,bp
R3786	T6235	T6232	compound	bp,band
R3787	T6236	T6201	cc	and,was
R3788	T6237	T6238	amod	knock,primers
R3789	T6238	T6201	conj	primers,was
R379	T815	T816	advmod	Remarkably,found
R3790	T6239	T6237	punct	-,knock
R3791	T6240	T6237	prt	in,knock
R3792	T6241	T6238	punct	", ",primers
R3793	T6242	T6238	appos	552F,primers
R3794	T6243	T6242	punct	(,552F
R3795	T6244	T6245	nummod	5,GAGCTTTGTTCTGGTTGCCTTC
R3796	T6245	T6242	appos	GAGCTTTGTTCTGGTTGCCTTC,552F
R3797	T6246	T6244	punct	',5
R3798	T6247	T6245	punct	-,GAGCTTTGTTCTGGTTGCCTTC
R3799	T6248	T6245	punct	-,GAGCTTTGTTCTGGTTGCCTTC
R38	T213	T214	nummod	1,kb
R380	T817	T816	punct	", ",found
R3800	T6249	T6245	nummod	3,GAGCTTTGTTCTGGTTGCCTTC
R3801	T6250	T6245	punct	',GAGCTTTGTTCTGGTTGCCTTC
R3802	T6251	T6242	punct	),552F
R3803	T6252	T6242	cc	and,552F
R3804	T6253	T6242	conj	Ex9RA,552F
R3805	T6254	T6253	punct	(,Ex9RA
R3806	T6255	T6256	nummod	5,GCAGTCTCTGCCTCGTTTTCT
R3807	T6256	T6253	appos	GCAGTCTCTGCCTCGTTTTCT,Ex9RA
R3808	T6257	T6255	punct	',5
R3809	T6258	T6256	punct	-,GCAGTCTCTGCCTCGTTTTCT
R381	T818	T819	det	these,deposits
R3810	T6259	T6256	punct	-,GCAGTCTCTGCCTCGTTTTCT
R3811	T6260	T6256	nummod	3,GCAGTCTCTGCCTCGTTTTCT
R3812	T6261	T6256	punct	',GCAGTCTCTGCCTCGTTTTCT
R3813	T6262	T6238	punct	),primers
R3814	T6263	T6264	aux	to,yield
R3815	T6264	T6238	advcl	yield,primers
R3816	T6265	T6266	det	a,band
R3817	T6266	T6264	dobj	band,yield
R3818	T6267	T6268	punct	~,500
R3819	T6268	T6269	nummod	500,bp
R382	T819	T816	nsubjpass	deposits,found
R3820	T6269	T6266	compound	bp,band
R3821	T6270	T6201	punct	.,was
R3822	T6272	T6273	compound	PCR,cycling
R3823	T6273	T6274	compound	cycling,conditions
R3824	T6274	T6275	nsubj	conditions,were
R3825	T6276	T6277	nummod	95,°C
R3826	T6277	T6275	attr	°C,were
R3827	T6278	T6277	prep	for,°C
R3828	T6279	T6280	nummod	30,seconds
R3829	T6280	T6278	pobj	seconds,for
R383	T820	T816	auxpass	are,found
R3830	T6281	T6277	punct	", ",°C
R3831	T6282	T6283	nummod	58,°C
R3832	T6283	T6277	conj	°C,°C
R3833	T6284	T6283	prep	for,°C
R3834	T6285	T6286	nummod	30,seconds
R3835	T6286	T6284	pobj	seconds,for
R3836	T6287	T6283	punct	", ",°C
R3837	T6288	T6283	cc	and,°C
R3838	T6289	T6290	nummod	72,°C
R3839	T6290	T6283	conj	°C,°C
R384	T821	T816	preconj	not,found
R3840	T6291	T6290	prep	for,°C
R3841	T6292	T6293	nummod	35,seconds
R3842	T6293	T6291	pobj	seconds,for
R3843	T6294	T6277	punct	", ",°C
R3844	T6295	T6277	acl	repeated,°C
R3845	T6296	T6295	prep	for,repeated
R3846	T6297	T6298	nummod	34,cycles
R3847	T6298	T6296	pobj	cycles,for
R3848	T6299	T6275	punct	.,were
R3849	T6301	T6302	amod	Total,isolation
R385	T822	T821	advmod	only,not
R3850	T6302	T6304	nsubjpass	isolation,described
R3851	T6303	T6302	compound	RNA,isolation
R3852	T6305	T6302	cc	and,isolation
R3853	T6306	T6307	compound	Cln3,primers
R3854	T6307	T6302	conj	primers,isolation
R3855	T6308	T6309	compound	RT,PCR
R3856	T6309	T6307	compound	PCR,primers
R3857	T6310	T6309	punct	-,PCR
R3858	T6311	T6307	cc	and,primers
R3859	T6312	T6307	conj	procedures,primers
R386	T823	T816	prep	in,found
R3860	T6313	T6304	aux	have,described
R3861	T6314	T6304	auxpass	been,described
R3862	T6315	T6304	advmod	previously,described
R3863	T6316	T6317	punct	[,12
R3864	T6317	T6304	parataxis	12,described
R3865	T6318	T6317	punct	],12
R3866	T6319	T6304	punct	.,described
R3867	T6369	T6370	compound	Subunit,c
R3868	T6370	T6371	compound	c,accumulation
R3869	T6371	T6372	compound	accumulation,assay
R387	T824	T825	compound	CNS,neurons
R3870	T6374	T6375	nsubjpass	Cells,seeded
R3871	T6376	T6375	auxpass	were,seeded
R3872	T6377	T6375	prep	into,seeded
R3873	T6378	T6379	nummod	4,well
R3874	T6379	T6381	compound	well,slides
R3875	T6380	T6379	punct	-,well
R3876	T6381	T6377	pobj	slides,into
R3877	T6382	T6381	compound	chamber,slides
R3878	T6383	T6381	punct	-,slides
R3879	T6384	T6385	punct	(,Falcon
R388	T825	T823	pobj	neurons,in
R3880	T6385	T6381	parataxis	Falcon,slides
R3881	T6386	T6385	punct	),Falcon
R3882	T6387	T6375	prep	at,seeded
R3883	T6388	T6389	det	a,density
R3884	T6389	T6387	pobj	density,at
R3885	T6390	T6389	prep	of,density
R3886	T6391	T6392	quantmod	5,104
R3887	T6392	T6394	nummod	104,cells
R3888	T6393	T6392	punct	×,104
R3889	T6394	T6390	pobj	cells,of
R389	T826	T816	cc	but,found
R3890	T6395	T6394	prep	per,cells
R3891	T6396	T6395	pobj	well,per
R3892	T6397	T6375	prep	for,seeded
R3893	T6398	T6399	compound	microscopy,studies
R3894	T6399	T6397	pobj	studies,for
R3895	T6400	T6375	punct	", ",seeded
R3896	T6401	T6375	cc	or,seeded
R3897	T6402	T6375	conj	into,seeded
R3898	T6403	T6404	nummod	100,mm
R3899	T6404	T6405	compound	mm,dishes
R39	T214	T211	compound	kb,mutation
R390	T827	T816	conj	are,found
R3900	T6405	T6402	pobj	dishes,into
R3901	T6406	T6407	punct	(,Falcon
R3902	T6407	T6405	parataxis	Falcon,dishes
R3903	T6408	T6407	punct	),Falcon
R3904	T6409	T6402	prep	at,into
R3905	T6410	T6411	det	a,density
R3906	T6411	T6409	pobj	density,at
R3907	T6412	T6411	prep	of,density
R3908	T6413	T6414	quantmod	5,105
R3909	T6414	T6416	nummod	105,cells
R391	T828	T827	advmod	also,are
R3910	T6415	T6414	punct	×,105
R3911	T6416	T6412	pobj	cells,of
R3912	T6417	T6416	prep	per,cells
R3913	T6418	T6417	pobj	dish,per
R3914	T6419	T6402	prep	for,into
R3915	T6420	T6421	compound	protein,extraction
R3916	T6421	T6419	pobj	extraction,for
R3917	T6422	T6375	punct	.,seeded
R3918	T6424	T6425	nsubj	Cells,were
R3919	T6426	T6425	advmod	typically,were
R392	T829	T827	acomp	abundant,are
R3920	T6427	T6428	punct	>,95
R3921	T6428	T6429	nummod	95,%
R3922	T6429	T6430	npadvmod	%,confluent
R3923	T6430	T6425	acomp	confluent,were
R3924	T6431	T6432	nummod	one,day
R3925	T6432	T6433	npadvmod	day,post-plating
R3926	T6433	T6425	advmod	post-plating,were
R3927	T6434	T6425	punct	", ",were
R3928	T6435	T6425	cc	and,were
R3929	T6436	T6437	det	the,day
R393	T830	T827	prep	in,are
R3930	T6437	T6439	nsubjpass	day,considered
R3931	T6438	T6437	amod	following,day
R3932	T6439	T6425	conj	considered,were
R3933	T6440	T6439	auxpass	was,considered
R3934	T6441	T6442	nummod	1,day
R3935	T6442	T6444	npadvmod	day,post-confluency
R3936	T6443	T6442	punct	-,day
R3937	T6444	T6439	advmod	post-confluency,considered
R3938	T6445	T6439	punct	.,considered
R3939	T6447	T6448	prep	At,fixed
R394	T831	T832	amod	non-neuronal,cells
R3940	T6449	T6450	det	the,times
R3941	T6450	T6447	pobj	times,At
R3942	T6451	T6450	amod	indicated,times
R3943	T6452	T6448	punct	", ",fixed
R3944	T6453	T6448	nsubjpass	cells,fixed
R3945	T6454	T6448	auxpass	were,fixed
R3946	T6455	T6448	advmod	either,fixed
R3947	T6456	T6448	prep	with,fixed
R3948	T6457	T6458	nummod	4,%
R3949	T6458	T6459	compound	%,formaldehyde
R395	T832	T830	pobj	cells,in
R3950	T6459	T6456	pobj	formaldehyde,with
R3951	T6460	T6448	prep	in,fixed
R3952	T6461	T6462	nmod	phosphate,saline
R3953	T6462	T6460	pobj	saline,in
R3954	T6463	T6462	amod	buffered,saline
R3955	T6464	T6462	punct	(,saline
R3956	T6465	T6462	appos	PBS,saline
R3957	T6466	T6462	punct	),saline
R3958	T6467	T6462	punct	", ",saline
R3959	T6468	T6462	npadvmod	pH,saline
R396	T833	T832	prep	outside,cells
R3960	T6469	T6468	nummod	7.4,pH
R3961	T6470	T6448	punct	", ",fixed
R3962	T6471	T6448	prep	for,fixed
R3963	T6472	T6473	nummod	20,minutes
R3964	T6473	T6471	pobj	minutes,for
R3965	T6474	T6448	cc	and,fixed
R3966	T6475	T6448	conj	processed,fixed
R3967	T6476	T6475	prep	for,processed
R3968	T6477	T6476	pobj	autofluorescence,for
R3969	T6478	T6477	punct	/,autofluorescence
R397	T834	T833	prep	of,outside
R3970	T6479	T6480	compound	subunit,c
R3971	T6480	T6481	compound	c,immunostaining
R3972	T6481	T6477	appos	immunostaining,autofluorescence
R3973	T6482	T6448	punct	", ",fixed
R3974	T6483	T6448	cc	or,fixed
R3975	T6484	T6485	compound	cell,pellets
R3976	T6485	T6486	nsubjpass	pellets,collected
R3977	T6486	T6448	conj	collected,fixed
R3978	T6487	T6486	auxpass	were,collected
R3979	T6488	T6486	prep	for,collected
R398	T835	T836	det	the,system
R3980	T6489	T6490	amod	total,extraction
R3981	T6490	T6488	pobj	extraction,for
R3982	T6491	T6490	compound	protein,extraction
R3983	T6492	T6486	punct	.,collected
R3984	T6494	T6495	advmod	Alternatively,fixed
R3985	T6496	T6495	punct	", ",fixed
R3986	T6497	T6495	nsubjpass	cells,fixed
R3987	T6498	T6495	auxpass	were,fixed
R3988	T6499	T6495	prep	with,fixed
R3989	T6500	T6501	nummod	2.5,%
R399	T836	T834	pobj	system,of
R3990	T6501	T6502	compound	%,glutaraldehyde
R3991	T6502	T6499	pobj	glutaraldehyde,with
R3992	T6503	T6502	punct	/,glutaraldehyde
R3993	T6504	T6505	nummod	2,%
R3994	T6505	T6506	compound	%,paraformaldehyde
R3995	T6506	T6502	appos	paraformaldehyde,glutaraldehyde
R3996	T6507	T6495	prep	in,fixed
R3997	T6508	T6509	nummod	0.1,M
R3998	T6509	T6510	compound	M,buffer
R3999	T6510	T6507	pobj	buffer,in
R4	T171	T173	nsubj	trafficking,precede
R40	T215	T211	compound	CLN3,mutation
R400	T837	T836	amod	nervous,system
R4000	T6511	T6510	compound	cacodylate,buffer
R4001	T6512	T6510	punct	", ",buffer
R4002	T6513	T6510	npadvmod	pH,buffer
R4003	T6514	T6513	nummod	7.4,pH
R4004	T6515	T6495	prep	for,fixed
R4005	T6516	T6517	nummod	1,hour
R4006	T6517	T6515	pobj	hour,for
R4007	T6518	T6495	cc	and,fixed
R4008	T6519	T6520	advmod	subsequently,post-fixed
R4009	T6520	T6495	conj	post-fixed,fixed
R401	T838	T816	punct	.,found
R4010	T6521	T6520	cc	and,post-fixed
R4011	T6522	T6520	conj	processed,post-fixed
R4012	T6523	T6522	prep	for,processed
R4013	T6524	T6525	compound	TEM,analysis
R4014	T6525	T6523	pobj	analysis,for
R4015	T6526	T6527	mark	as,described
R4016	T6527	T6522	advcl	described,processed
R4017	T6528	T6529	punct	[,12
R4018	T6529	T6527	parataxis	12,described
R4019	T6530	T6529	punct	],12
R402	T840	T841	det	The,relationship
R4020	T6531	T6495	punct	.,fixed
R4021	T6533	T6534	prep	In,observed
R4022	T6535	T6536	amod	confocal,studies
R4023	T6536	T6533	pobj	studies,In
R4024	T6537	T6536	compound	microscopy,studies
R4025	T6538	T6534	punct	", ",observed
R4026	T6539	T6540	amod	autofluorescent,signal
R4027	T6540	T6534	nsubjpass	signal,observed
R4028	T6541	T6534	auxpass	was,observed
R4029	T6542	T6534	prep	over,observed
R403	T841	T842	nsubj	relationship,remain
R4030	T6543	T6544	amod	multiple,wavelengths
R4031	T6544	T6542	pobj	wavelengths,over
R4032	T6545	T6534	punct	.,observed
R4033	T6547	T6548	prep	For,reduced
R4034	T6549	T6547	pobj	co-staining,For
R4035	T6550	T6548	punct	", ",reduced
R4036	T6551	T6548	nsubjpass	settings,reduced
R4037	T6552	T6548	auxpass	were,reduced
R4038	T6553	T6548	advcl	such,reduced
R4039	T6554	T6555	mark	that,contribute
R404	T843	T841	prep	of,relationship
R4040	T6555	T6553	ccomp	contribute,such
R4041	T6556	T6557	amod	autofluorescent,signal
R4042	T6557	T6555	nsubj	signal,contribute
R4043	T6558	T6555	aux	did,contribute
R4044	T6559	T6555	neg	not,contribute
R4045	T6560	T6555	prep	to,contribute
R4046	T6561	T6562	compound	antibody,signal
R4047	T6562	T6560	pobj	signal,to
R4048	T6563	T6562	compound	label,signal
R4049	T6564	T6548	punct	.,reduced
R405	T844	T845	compound	subunit,c
R4050	T6658	T6659	nmod	Immunostaining,analysis
R4051	T6660	T6658	cc	and,Immunostaining
R4052	T6661	T6658	conj	Immunoblot,Immunostaining
R4053	T6663	T6664	prep	For,seeded
R4054	T6665	T6663	pobj	immunostaining,For
R4055	T6666	T6664	punct	", ",seeded
R4056	T6667	T6664	nsubj	cells,seeded
R4057	T6668	T6664	aux	were,seeded
R4058	T6669	T6664	prep	at,seeded
R4059	T6670	T6671	det	a,density
R406	T845	T846	compound	c,deposits
R4060	T6671	T6669	pobj	density,at
R4061	T6672	T6671	prep	of,density
R4062	T6673	T6674	quantmod	3,5
R4063	T6674	T6676	quantmod	5,104
R4064	T6675	T6674	punct	–,5
R4065	T6676	T6678	nummod	104,cells
R4066	T6677	T6676	punct	×,104
R4067	T6678	T6672	pobj	cells,of
R4068	T6679	T6678	prep	per,cells
R4069	T6680	T6679	pobj	well,per
R407	T846	T843	pobj	deposits,of
R4070	T6681	T6664	prep	in,seeded
R4071	T6682	T6683	nummod	4,well
R4072	T6683	T6685	compound	well,slides
R4073	T6684	T6683	punct	-,well
R4074	T6685	T6681	pobj	slides,in
R4075	T6686	T6685	compound	chamber,slides
R4076	T6687	T6685	punct	-,slides
R4077	T6688	T6664	cc	and,seeded
R4078	T6689	T6664	conj	grown,seeded
R4079	T6690	T6689	advmod	overnight,grown
R408	T847	T841	prep	to,relationship
R4080	T6691	T6689	prep	at,grown
R4081	T6692	T6693	nummod	33,°C
R4082	T6693	T6691	pobj	°C,at
R4083	T6694	T6664	punct	", ",seeded
R4084	T6695	T6696	mark	unless,indicated
R4085	T6696	T6664	advcl	indicated,seeded
R4086	T6697	T6696	advmod	otherwise,indicated
R4087	T6698	T6664	punct	.,seeded
R4088	T6700	T6701	nsubj	Fixation,was
R4089	T6702	T6701	prep	with,was
R409	T848	T849	det	the,process
R4090	T6703	T6704	npadvmod	ice,cold
R4091	T6704	T6706	amod	cold,formaldehyde
R4092	T6705	T6704	punct	-,cold
R4093	T6706	T6702	pobj	formaldehyde,with
R4094	T6707	T6708	nummod	4,%
R4095	T6708	T6706	compound	%,formaldehyde
R4096	T6709	T6706	prep	in,formaldehyde
R4097	T6710	T6709	pobj	PBS,in
R4098	T6711	T6710	punct	", ",PBS
R4099	T6712	T6710	npadvmod	pH,PBS
R41	T216	T195	punct	.,is
R410	T849	T847	pobj	process,to
R4100	T6713	T6712	nummod	7.4,pH
R4101	T6714	T6706	punct	", ",formaldehyde
R4102	T6715	T6706	prep	for,formaldehyde
R4103	T6716	T6717	nummod	20,minutes
R4104	T6717	T6715	pobj	minutes,for
R4105	T6718	T6702	punct	", ",with
R4106	T6719	T6702	cc	or,with
R4107	T6720	T6702	conj	with,with
R4108	T6721	T6722	npadvmod	ice,cold
R4109	T6722	T6724	amod	cold,acetone
R411	T850	T851	compound	JNCL,disease
R4110	T6723	T6722	punct	-,cold
R4111	T6724	T6720	pobj	acetone,with
R4112	T6725	T6724	compound	methanol,acetone
R4113	T6726	T6724	punct	/,acetone
R4114	T6727	T6728	punct	(,1
R4115	T6728	T6724	parataxis	1,acetone
R4116	T6729	T6730	punct	:,1
R4117	T6730	T6728	prep	1,1
R4118	T6731	T6728	punct	),1
R4119	T6732	T6724	prep	for,acetone
R412	T851	T849	compound	disease,process
R4120	T6733	T6734	nummod	10,minutes
R4121	T6734	T6732	pobj	minutes,for
R4122	T6735	T6724	prep	at,acetone
R4123	T6736	T6737	punct	-,20
R4124	T6737	T6738	nummod	20,°C
R4125	T6738	T6735	pobj	°C,at
R4126	T6739	T6724	acl	followed,acetone
R4127	T6740	T6739	agent	by,followed
R4128	T6741	T6742	compound	air,drying
R4129	T6742	T6740	pobj	drying,by
R413	T852	T841	punct	", ",relationship
R4130	T6743	T6742	punct	-,drying
R4131	T6744	T6745	punct	(,dependent
R4132	T6745	T6739	parataxis	dependent,followed
R4133	T6746	T6745	npadvmod	antibody,dependent
R4134	T6747	T6745	punct	-,dependent
R4135	T6748	T6745	punct	),dependent
R4136	T6749	T6701	punct	.,was
R4137	T6751	T6752	nsubjpass	Cells,washed
R4138	T6753	T6752	auxpass	were,washed
R4139	T6754	T6752	prep	with,washed
R414	T853	T841	cc	and,relationship
R4140	T6755	T6754	pobj	PBS,with
R4141	T6756	T6757	advmod	at,2
R4142	T6757	T6752	npadvmod	2,washed
R4143	T6758	T6757	advmod	least,2
R4144	T6759	T6757	quantmod	times,2
R4145	T6760	T6752	punct	", ",washed
R4146	T6761	T6762	nummod	5,minutes
R4147	T6762	T6752	npadvmod	minutes,washed
R4148	T6763	T6762	prep	per,minutes
R4149	T6764	T6763	pobj	wash,per
R415	T854	T855	det	the,reason
R4150	T6765	T6752	punct	", ",washed
R4151	T6766	T6752	prep	between,washed
R4152	T6767	T6766	pobj	each,between
R4153	T6768	T6767	prep	of,each
R4154	T6769	T6770	det	the,steps
R4155	T6770	T6768	pobj	steps,of
R4156	T6771	T6770	amod	following,steps
R4157	T6772	T6770	prep	of,steps
R4158	T6773	T6774	det	the,procedure
R4159	T6774	T6772	pobj	procedure,of
R416	T855	T841	conj	reason,relationship
R4160	T6775	T6774	compound	staining,procedure
R4161	T6776	T6770	punct	: ,steps
R4162	T6777	T6778	nummod	0.1,M
R4163	T6778	T6779	compound	M,glycine
R4164	T6779	T6770	appos	glycine,steps
R4165	T6780	T6779	prep	in,glycine
R4166	T6781	T6780	pobj	PBS,in
R4167	T6782	T6779	prep	for,glycine
R4168	T6783	T6784	nummod	5,minutes
R4169	T6784	T6782	pobj	minutes,for
R417	T856	T855	amod	underlying,reason
R4170	T6785	T6779	punct	", ",glycine
R4171	T6786	T6787	nummod	0.05,%
R4172	T6787	T6788	nmod	%,X
R4173	T6788	T6779	appos	X,glycine
R4174	T6789	T6787	cc	or,%
R4175	T6790	T6791	nummod	0.1,%
R4176	T6791	T6787	conj	%,%
R4177	T6792	T6788	punct	(,X
R4178	T6793	T6794	npadvmod	antibody,dependent
R4179	T6794	T6788	amod	dependent,X
R418	T857	T855	prep	for,reason
R4180	T6795	T6794	punct	-,dependent
R4181	T6796	T6788	punct	),X
R4182	T6797	T6788	compound	Triton,X
R4183	T6798	T6788	punct	-,X
R4184	T6799	T6788	nummod	100,X
R4185	T6800	T6801	punct	(,Scientific
R4186	T6801	T6788	parataxis	Scientific,X
R4187	T6802	T6801	compound	Fisher,Scientific
R4188	T6803	T6801	punct	),Scientific
R4189	T6804	T6788	prep	in,X
R419	T858	T859	det	the,specificity
R4190	T6805	T6804	pobj	PBS,in
R4191	T6806	T6788	prep	for,X
R4192	T6807	T6808	nummod	5,minutes
R4193	T6808	T6806	pobj	minutes,for
R4194	T6809	T6779	punct	", ",glycine
R4195	T6810	T6811	nummod	2,%
R4196	T6811	T6779	appos	%,glycine
R4197	T6812	T6813	amod	bovine,albumin
R4198	T6813	T6811	appos	albumin,%
R4199	T6814	T6813	compound	serum,albumin
R42	T218	T219	det	The,loss
R420	T859	T857	pobj	specificity,for
R4200	T6815	T6813	punct	(,albumin
R4201	T6816	T6813	appos	BSA,albumin
R4202	T6817	T6811	punct	),%
R4203	T6818	T6811	prep	in,%
R4204	T6819	T6818	pobj	PBS,in
R4205	T6820	T6811	prep	for,%
R4206	T6821	T6822	nummod	30,minutes
R4207	T6822	T6820	pobj	minutes,for
R4208	T6823	T6779	punct	", ",glycine
R4209	T6824	T6825	amod	primary,antibody
R421	T860	T859	amod	neuronal,specificity
R4210	T6825	T6779	appos	antibody,glycine
R4211	T6826	T6825	acl	diluted,antibody
R4212	T6827	T6826	prep	in,diluted
R4213	T6828	T6829	nummod	2,%
R4214	T6829	T6830	compound	%,PBS
R4215	T6830	T6827	pobj	PBS,in
R4216	T6831	T6830	compound	BSA,PBS
R4217	T6832	T6830	punct	/,PBS
R4218	T6833	T6826	prep	for,diluted
R4219	T6834	T6835	nummod	90,minutes
R422	T861	T859	prep	of,specificity
R4220	T6835	T6833	pobj	minutes,for
R4221	T6836	T6779	punct	", ",glycine
R4222	T6837	T6838	amod	secondary,antibody
R4223	T6838	T6779	appos	antibody,glycine
R4224	T6839	T6838	acl	diluted,antibody
R4225	T6840	T6839	prep	in,diluted
R4226	T6841	T6842	nummod	2,%
R4227	T6842	T6843	compound	%,PBS
R4228	T6843	T6840	pobj	PBS,in
R4229	T6844	T6843	compound	BSA,PBS
R423	T862	T863	det	the,disease
R4230	T6845	T6843	punct	/,PBS
R4231	T6846	T6839	prep	for,diluted
R4232	T6847	T6848	nummod	60,minutes
R4233	T6848	T6846	pobj	minutes,for
R4234	T6849	T6752	punct	.,washed
R4235	T6851	T6852	det	All,incubations
R4236	T6852	T6853	nsubjpass	incubations,carried
R4237	T6854	T6853	auxpass	were,carried
R4238	T6855	T6853	prt	out,carried
R4239	T6856	T6853	prep	at,carried
R424	T863	T861	pobj	disease,of
R4240	T6857	T6858	compound	room,temperature
R4241	T6858	T6856	pobj	temperature,at
R4242	T6859	T6853	punct	.,carried
R4243	T6861	T6862	prep	Following,coverslipped
R4244	T6863	T6864	compound	staining,procedures
R4245	T6864	T6861	pobj	procedures,Following
R4246	T6865	T6862	punct	", ",coverslipped
R4247	T6866	T6862	nsubjpass	slides,coverslipped
R4248	T6867	T6862	auxpass	were,coverslipped
R4249	T6868	T6862	prep	with,coverslipped
R425	T864	T865	advmod	poorly,understood
R4250	T6869	T6870	compound	Vectashield,medium
R4251	T6870	T6868	pobj	medium,with
R4252	T6871	T6870	compound	mounting,medium
R4253	T6872	T6873	punct	(,Laboratories
R4254	T6873	T6870	parataxis	Laboratories,medium
R4255	T6874	T6873	compound	Vector,Laboratories
R4256	T6875	T6873	punct	),Laboratories
R4257	T6876	T6862	cc	and,coverslipped
R4258	T6877	T6862	conj	analyzed,coverslipped
R4259	T6878	T6877	prep	on,analyzed
R426	T865	T842	acomp	understood,remain
R4260	T6879	T6880	det	a,microscope
R4261	T6880	T6878	pobj	microscope,on
R4262	T6881	T6882	nmod	BioRad,Radiance
R4263	T6882	T6880	nmod	Radiance,microscope
R4264	T6883	T6882	nummod	2100,Radiance
R4265	T6884	T6880	amod	confocal,microscope
R4266	T6885	T6886	punct	(,Biorad
R4267	T6886	T6880	parataxis	Biorad,microscope
R4268	T6887	T6886	punct	),Biorad
R4269	T6888	T6877	punct	", ",analyzed
R427	T866	T842	punct	.,remain
R4270	T6889	T6877	prep	with,analyzed
R4271	T6890	T6891	amod	identical,settings
R4272	T6891	T6889	pobj	settings,with
R4273	T6892	T6891	compound	exposure,settings
R4274	T6893	T6891	prep	for,settings
R4275	T6894	T6895	amod	wild,type
R4276	T6895	T6897	nmod	type,images
R4277	T6896	T6895	punct	-,type
R4278	T6897	T6893	pobj	images,for
R4279	T6898	T6895	cc	and,type
R428	T868	T869	det	The,protein
R4280	T6899	T6895	conj	mutant,type
R4281	T6900	T6897	amod	like,images
R4282	T6901	T6862	punct	.,coverslipped
R4283	T6903	T6904	det	All,comparisons
R4284	T6904	T6905	nsubjpass	comparisons,performed
R4285	T6906	T6904	prep	of,comparisons
R4286	T6907	T6908	amod	wild,type
R4287	T6908	T6910	nmod	type,staining
R4288	T6909	T6908	punct	-,type
R4289	T6910	T6906	pobj	staining,of
R429	T869	T873	nsubj	protein,is
R4290	T6911	T6908	cc	and,type
R4291	T6912	T6908	conj	mutant,type
R4292	T6913	T6905	auxpass	were,performed
R4293	T6914	T6905	prep	in,performed
R4294	T6915	T6916	compound	Adobe,Photoshop
R4295	T6916	T6914	pobj	Photoshop,in
R4296	T6917	T6905	prep	with,performed
R4297	T6918	T6919	amod	identical,adjustments
R4298	T6919	T6917	pobj	adjustments,with
R4299	T6920	T6919	nmod	brightness,adjustments
R43	T219	T221	nsubj	loss,leads
R430	T870	T871	npadvmod	CLN3,encoded
R4300	T6921	T6920	cc	and,brightness
R4301	T6922	T6920	conj	contrast,brightness
R4302	T6923	T6905	punct	.,performed
R4303	T6925	T6926	amod	Total,proteins
R4304	T6926	T6927	nsubjpass	proteins,isolated
R4305	T6928	T6927	auxpass	were,isolated
R4306	T6929	T6927	prep	from,isolated
R4307	T6930	T6931	compound	cell,pellets
R4308	T6931	T6929	pobj	pellets,from
R4309	T6932	T6927	prep	by,isolated
R431	T871	T869	amod	encoded,protein
R4310	T6933	T6932	pobj	extraction,by
R4311	T6934	T6933	prep	with,extraction
R4312	T6935	T6936	nmod	ice,Tris
R4313	T6936	T6934	pobj	Tris,with
R4314	T6937	T6935	punct	-,ice
R4315	T6938	T6935	amod	cold,ice
R4316	T6939	T6940	nummod	20,mM
R4317	T6940	T6936	compound	mM,Tris
R4318	T6941	T6936	punct	", ",Tris
R4319	T6942	T6936	npadvmod	pH,Tris
R432	T872	T871	punct	-,encoded
R4320	T6943	T6942	nummod	7.4,pH
R4321	T6944	T6936	punct	", ",Tris
R4322	T6945	T6946	nummod	1,%
R4323	T6946	T6947	compound	%,X
R4324	T6947	T6936	conj	X,Tris
R4325	T6948	T6947	compound	Triton,X
R4326	T6949	T6947	punct	-,X
R4327	T6950	T6947	nummod	100,X
R4328	T6951	T6952	punct	(,Roche
R4329	T6952	T6947	parataxis	Roche,X
R433	T874	T869	punct	(,protein
R4330	T6953	T6954	compound	membrane,research
R4331	T6954	T6956	compound	research,grade
R4332	T6955	T6954	punct	-,research
R4333	T6956	T6952	dep	grade,Roche
R4334	T6957	T6952	punct	", ",Roche
R4335	T6958	T6952	punct	),Roche
R4336	T6959	T6947	punct	", ",X
R4337	T6960	T6947	cc	plus,X
R4338	T6961	T6962	compound	protease,inhibitors
R4339	T6962	T6947	conj	inhibitors,X
R434	T875	T869	appos	battenin,protein
R4340	T6963	T6962	punct	(,inhibitors
R4341	T6964	T6965	amod	Complete,tablet
R4342	T6965	T6962	appos	tablet,inhibitors
R4343	T6966	T6965	compound	mini,tablet
R4344	T6967	T6965	punct	", ",tablet
R4345	T6968	T6969	nummod	0.7,μg
R4346	T6969	T6970	nmod	μg,A
R4347	T6970	T6965	appos	A,tablet
R4348	T6971	T6972	punct	/,ml
R4349	T6972	T6969	prep	ml,μg
R435	T876	T875	punct	", ",battenin
R4350	T6973	T6970	compound	pepstatin,A
R4351	T6974	T6965	punct	", ",tablet
R4352	T6975	T6976	nummod	2,μg
R4353	T6976	T6977	nmod	μg,aprotinin
R4354	T6977	T6965	appos	aprotinin,tablet
R4355	T6978	T6979	punct	/,ml
R4356	T6979	T6976	prep	ml,μg
R4357	T6980	T6965	punct	", ",tablet
R4358	T6981	T6982	nummod	5,μg
R4359	T6982	T6983	nmod	μg,leupeptin
R436	T877	T878	advmod	also,called
R4360	T6983	T6965	appos	leupeptin,tablet
R4361	T6984	T6985	punct	/,ml
R4362	T6985	T6982	prep	ml,μg
R4363	T6986	T6987	punct	[,Roche
R4364	T6987	T6983	parataxis	Roche,leupeptin
R4365	T6988	T6987	punct	],Roche
R4366	T6989	T6927	punct	),isolated
R4367	T6990	T6927	punct	.,isolated
R4368	T6992	T6993	prep	Following,centrifuged
R4369	T6994	T6992	pobj	homogenization,Following
R437	T878	T875	acl	called,battenin
R4370	T6995	T6994	prep	through,homogenization
R4371	T6996	T6997	det	a,needle
R4372	T6997	T6995	pobj	needle,through
R4373	T6998	T6999	nummod	25,gauge
R4374	T6999	T6997	compound	gauge,needle
R4375	T7000	T6999	punct	-,gauge
R4376	T7001	T7002	punct	(,passes
R4377	T7002	T6994	parataxis	passes,homogenization
R4378	T7003	T7004	punct	~,10
R4379	T7004	T7002	nummod	10,passes
R438	T879	T878	oprd	CLN3,called
R4380	T7005	T7002	punct	),passes
R4381	T7006	T6993	punct	", ",centrifuged
R4382	T7007	T6993	nsubjpass	extracts,centrifuged
R4383	T7008	T6993	auxpass	were,centrifuged
R4384	T7009	T6993	prep	at,centrifuged
R4385	T7010	T7011	nummod	1000,g
R4386	T7011	T7009	pobj	g,at
R4387	T7012	T7011	punct	×,g
R4388	T7013	T6993	prep	for,centrifuged
R4389	T7014	T7015	nummod	10,minutes
R439	T880	T879	cc	or,CLN3
R4390	T7015	T7013	pobj	minutes,for
R4391	T7016	T6993	punct	", ",centrifuged
R4392	T7017	T6993	prep	at,centrifuged
R4393	T7018	T7019	nummod	4,°C
R4394	T7019	T7017	pobj	°C,at
R4395	T7020	T6993	punct	", ",centrifuged
R4396	T7021	T7022	aux	to,remove
R4397	T7022	T6993	advcl	remove,centrifuged
R4398	T7023	T7022	dobj	debris,remove
R4399	T7024	T6993	punct	.,centrifuged
R44	T220	T219	amod	resulting,loss
R440	T881	T882	compound	cln3,p
R4400	T7026	T7027	advmod	Typically,separated
R4401	T7028	T7027	punct	", ",separated
R4402	T7029	T7030	quantmod	20,40
R4403	T7030	T7032	nummod	40,μg
R4404	T7031	T7030	punct	–,40
R4405	T7032	T7027	nsubjpass	μg,separated
R4406	T7033	T7032	prep	of,μg
R4407	T7034	T7033	pobj	protein,of
R4408	T7035	T7034	punct	(,protein
R4409	T7036	T7034	acl	determined,protein
R441	T882	T879	conj	p,CLN3
R4410	T7037	T7036	agent	by,determined
R4411	T7038	T7039	compound	Bio,rad
R4412	T7039	T7041	compound	rad,Assay
R4413	T7040	T7039	punct	-,rad
R4414	T7041	T7037	pobj	Assay,by
R4415	T7042	T7041	compound	Dc,Assay
R4416	T7043	T7041	compound	Protein,Assay
R4417	T7044	T7027	punct	),separated
R4418	T7045	T7027	auxpass	was,separated
R4419	T7046	T7027	prep	by,separated
R442	T883	T873	punct	),is
R4420	T7047	T7048	compound	SDS,PAGE
R4421	T7048	T7046	pobj	PAGE,by
R4422	T7049	T7048	punct	-,PAGE
R4423	T7050	T7027	punct	", ",separated
R4424	T7051	T7027	prep	for,separated
R4425	T7052	T7053	amod	subsequent,immunoblotting
R4426	T7053	T7051	pobj	immunoblotting,for
R4427	T7054	T7027	punct	", ",separated
R4428	T7055	T7056	mark	as,described
R4429	T7056	T7027	advcl	described,separated
R443	T884	T885	det	a,protein
R4430	T7057	T7058	punct	[,12
R4431	T7058	T7027	parataxis	12,separated
R4432	T7059	T7058	punct	],12
R4433	T7060	T7027	punct	.,separated
R4434	T7062	T7063	nummod	16.5,%
R4435	T7063	T7064	compound	%,gels
R4436	T7064	T7071	nsubjpass	gels,used
R4437	T7065	T7066	compound	tris,tricine
R4438	T7066	T7064	compound	tricine,gels
R4439	T7067	T7066	punct	-,tricine
R444	T885	T873	attr	protein,is
R4440	T7068	T7069	compound	SDS,PAGE
R4441	T7069	T7064	compound	PAGE,gels
R4442	T7070	T7069	punct	-,PAGE
R4443	T7072	T7071	auxpass	were,used
R4444	T7073	T7071	prep	for,used
R4445	T7074	T7075	compound	subunit,c
R4446	T7075	T7076	compound	c,experiments
R4447	T7076	T7073	pobj	experiments,for
R4448	T7077	T7076	compound	immunoblotting,experiments
R4449	T7078	T7071	punct	.,used
R445	T886	T887	advmod	highly,conserved
R4450	T7116	T7117	compound	Cathepsin,D
R4451	T7117	T7118	compound	D,assay
R4452	T7119	T7118	compound	activity,assay
R4453	T7121	T7122	nummod	100,mm
R4454	T7122	T7123	compound	mm,dishes
R4455	T7123	T7126	nsubjpass	dishes,washed
R4456	T7124	T7125	compound	tissue,culture
R4457	T7125	T7123	compound	culture,dishes
R4458	T7127	T7123	punct	", ",dishes
R4459	T7128	T7129	dep	which,were
R446	T887	T885	amod	conserved,protein
R4460	T7129	T7123	relcl	were,dishes
R4461	T7130	T7131	advmod	approximately,90
R4462	T7131	T7134	nummod	90,%
R4463	T7132	T7131	quantmod	80,90
R4464	T7133	T7131	punct	–,90
R4465	T7134	T7135	npadvmod	%,confluent
R4466	T7135	T7129	acomp	confluent,were
R4467	T7136	T7126	punct	", ",washed
R4468	T7137	T7126	auxpass	were,washed
R4469	T7138	T7126	advmod	briefly,washed
R447	T888	T885	punct	", ",protein
R4470	T7139	T7126	prep	with,washed
R4471	T7140	T7141	npadvmod	ice,cold
R4472	T7141	T7143	amod	cold,PBS
R4473	T7142	T7141	punct	-,cold
R4474	T7143	T7139	pobj	PBS,with
R4475	T7144	T7126	punct	", ",washed
R4476	T7145	T7126	cc	and,washed
R4477	T7146	T7147	amod	total,extracts
R4478	T7147	T7149	nsubjpass	extracts,isolated
R4479	T7148	T7147	compound	protein,extracts
R448	T889	T890	advmod	ubiquitously,expressed
R4480	T7149	T7126	conj	isolated,washed
R4481	T7150	T7149	auxpass	were,isolated
R4482	T7151	T7149	prep	by,isolated
R4483	T7152	T7151	pcomp	scraping,by
R4484	T7153	T7152	dobj	cells,scraping
R4485	T7154	T7152	prep	into,scraping
R4486	T7155	T7156	nummod	10,mM
R4487	T7156	T7157	compound	mM,Tris
R4488	T7157	T7154	pobj	Tris,into
R4489	T7158	T7157	punct	", ",Tris
R449	T890	T885	amod	expressed,protein
R4490	T7159	T7157	npadvmod	pH,Tris
R4491	T7160	T7159	nummod	7.4,pH
R4492	T7161	T7157	punct	", ",Tris
R4493	T7162	T7163	nummod	0.1,%
R4494	T7163	T7164	compound	%,X
R4495	T7164	T7157	appos	X,Tris
R4496	T7165	T7164	compound	Triton,X
R4497	T7166	T7164	punct	-,X
R4498	T7167	T7164	nummod	100,X
R4499	T7168	T7157	acl	followed,Tris
R45	T222	T219	prep	of,loss
R450	T891	T885	punct	", ",protein
R4500	T7169	T7168	agent	by,followed
R4501	T7170	T7169	pobj	incubation,by
R4502	T7171	T7170	prep	on,incubation
R4503	T7172	T7171	pobj	ice,on
R4504	T7173	T7170	punct	", ",incubation
R4505	T7174	T7170	prep	for,incubation
R4506	T7175	T7176	nummod	20,minutes
R4507	T7176	T7174	pobj	minutes,for
R4508	T7177	T7149	punct	.,isolated
R4509	T7179	T7180	det	The,material
R451	T892	T885	compound	multi-pass,protein
R4510	T7180	T7182	nsubjpass	material,centrifuged
R4511	T7181	T7180	amod	insoluble,material
R4512	T7183	T7182	auxpass	was,centrifuged
R4513	T7184	T7182	prep	at,centrifuged
R4514	T7185	T7186	nummod	"14,000",g
R4515	T7186	T7184	pobj	g,at
R4516	T7187	T7182	punct	", ",centrifuged
R4517	T7188	T7189	det	the,supernatant
R4518	T7189	T7190	nsubjpass	supernatant,isolated
R4519	T7190	T7182	conj	isolated,centrifuged
R452	T893	T885	compound	membrane,protein
R4520	T7191	T7190	auxpass	was,isolated
R4521	T7192	T7190	punct	", ",isolated
R4522	T7193	T7190	cc	and,isolated
R4523	T7194	T7195	compound	protein,concentration
R4524	T7195	T7196	nsubjpass	concentration,determined
R4525	T7196	T7190	conj	determined,isolated
R4526	T7197	T7196	auxpass	was,determined
R4527	T7198	T7196	advcl	using,determined
R4528	T7199	T7200	det	the,Assay
R4529	T7200	T7198	dobj	Assay,using
R453	T894	T895	punct	[,6
R4530	T7201	T7202	compound	Bio,rad
R4531	T7202	T7200	compound	rad,Assay
R4532	T7203	T7202	punct	-,rad
R4533	T7204	T7200	compound	Dc,Assay
R4534	T7205	T7200	compound	Protein,Assay
R4535	T7206	T7196	punct	.,determined
R4536	T7208	T7209	quantmod	50,70
R4537	T7209	T7211	nummod	70,μg
R4538	T7210	T7209	punct	–,70
R4539	T7211	T7212	nsubjpass	μg,used
R454	T895	T885	parataxis	6,protein
R4540	T7213	T7211	prep	of,μg
R4541	T7214	T7215	amod	total,extract
R4542	T7215	T7213	pobj	extract,of
R4543	T7216	T7215	compound	protein,extract
R4544	T7217	T7212	auxpass	were,used
R4545	T7218	T7219	aux	to,measure
R4546	T7219	T7212	advcl	measure,used
R4547	T7220	T7221	compound	cathepsin,D
R4548	T7221	T7222	compound	D,activity
R4549	T7222	T7219	dobj	activity,measure
R455	T896	T895	punct	],6
R4550	T7223	T7219	advcl	using,measure
R4551	T7224	T7225	det	the,Kit
R4552	T7225	T7223	dobj	Kit,using
R4553	T7226	T7227	amod	Fluorogenic,D
R4554	T7227	T7225	compound	D,Kit
R4555	T7228	T7227	nmod	Innozyme,D
R4556	T7229	T7227	punct	™,D
R4557	T7230	T7227	compound	Cathepsin,D
R4558	T7231	T7225	compound	Immunocapture,Kit
R4559	T7232	T7225	compound	Activity,Kit
R456	T897	T898	dep	that,localizes
R4560	T7233	T7225	compound	Assay,Kit
R4561	T7234	T7235	punct	(,Biosciences
R4562	T7235	T7225	parataxis	Biosciences,Kit
R4563	T7236	T7235	compound	EMD,Biosciences
R4564	T7237	T7235	punct	),Biosciences
R4565	T7238	T7223	prep	according,using
R4566	T7239	T7238	prep	to,according
R4567	T7240	T7241	det	the,manufacturer
R4568	T7241	T7242	poss	manufacturer,recommendations
R4569	T7242	T7239	pobj	recommendations,to
R457	T898	T885	relcl	localizes,protein
R4570	T7243	T7241	case	's,manufacturer
R4571	T7244	T7212	punct	.,used
R4572	T7246	T7247	amod	Relative,fluorescence
R4573	T7247	T7248	nsubjpass	fluorescence,measured
R4574	T7249	T7248	auxpass	was,measured
R4575	T7250	T7248	advcl	using,measured
R4576	T7251	T7252	det	an,reader
R4577	T7252	T7250	dobj	reader,using
R4578	T7253	T7254	compound	Analyst,AD
R4579	T7254	T7252	compound	AD,reader
R458	T899	T898	prep	to,localizes
R4580	T7255	T7252	compound	plate,reader
R4581	T7256	T7257	punct	(,Devices
R4582	T7257	T7252	parataxis	Devices,reader
R4583	T7258	T7257	amod	Molecular,Devices
R4584	T7259	T7257	punct	),Devices
R4585	T7260	T7250	prep	with,using
R4586	T7261	T7262	det	the,filters
R4587	T7262	T7260	pobj	filters,with
R4588	T7263	T7262	amod	following,filters
R4589	T7264	T7262	cc	and,filters
R459	T900	T901	det	the,lysosome
R4590	T7265	T7262	conj	settings,filters
R4591	T7266	T7248	punct	: ,measured
R4592	T7267	T7268	compound	excitation,filter
R4593	T7268	T7269	dep	filter,35
R4594	T7269	T7248	dep	35,measured
R4595	T7270	T7269	punct	", ",35
R4596	T7271	T7269	nummod	360,35
R4597	T7272	T7269	punct	-,35
R4598	T7273	T7269	punct	;,35
R4599	T7274	T7275	compound	emission,filter
R46	T223	T224	compound	battenin,activity
R460	T901	T899	pobj	lysosome,to
R4600	T7275	T7276	dep	filter,20
R4601	T7276	T7269	conj	20,35
R4602	T7277	T7276	punct	", ",20
R4603	T7278	T7276	nummod	400,20
R4604	T7279	T7276	punct	-,20
R4605	T7280	T7276	punct	", ",20
R4606	T7281	T7282	compound	Flash,lamp
R4607	T7282	T7276	conj	lamp,20
R4608	T7283	T7282	prep	with,lamp
R4609	T7284	T7285	nummod	100,readings
R461	T902	T901	cc	and,lysosome
R4610	T7285	T7283	pobj	readings,with
R4611	T7286	T7287	punct	/,well
R4612	T7287	T7285	prep	well,readings
R4613	T7288	T7282	punct	", ",lamp
R4614	T7289	T7290	nummod	100,ms
R4615	T7290	T7282	conj	ms,lamp
R4616	T7291	T7290	prep	between,ms
R4617	T7292	T7291	pobj	readings,between
R4618	T7293	T7290	punct	", ",ms
R4619	T7294	T7290	cc	and,ms
R462	T903	T904	amod	other,compartments
R4620	T7295	T7296	nummod	"100,000",μs
R4621	T7296	T7297	compound	μs,time
R4622	T7297	T7290	conj	time,ms
R4623	T7298	T7297	compound	integration,time
R4624	T7299	T7248	punct	.,measured
R4625	T7337	T7338	amod	Lysosomal,staining
R4626	T7339	T7338	cc	and,staining
R4627	T7340	T7341	amod	endocytic,uptake
R4628	T7341	T7338	conj	uptake,staining
R4629	T7343	T7344	nsubjpass	Cells,seeded
R463	T904	T901	conj	compartments,lysosome
R4630	T7345	T7344	auxpass	were,seeded
R4631	T7346	T7344	prep	at,seeded
R4632	T7347	T7348	det	a,density
R4633	T7348	T7346	pobj	density,at
R4634	T7349	T7348	prep	of,density
R4635	T7350	T7351	quantmod	3,5
R4636	T7351	T7353	quantmod	5,104
R4637	T7352	T7351	punct	–,5
R4638	T7353	T7355	nummod	104,cells
R4639	T7354	T7353	punct	×,104
R464	T905	T904	amod	vesicular,compartments
R4640	T7355	T7349	pobj	cells,of
R4641	T7356	T7355	prep	per,cells
R4642	T7357	T7356	pobj	well,per
R4643	T7358	T7344	prep	in,seeded
R4644	T7359	T7360	nummod	4,well
R4645	T7360	T7362	compound	well,slides
R4646	T7361	T7360	punct	-,well
R4647	T7362	T7358	pobj	slides,in
R4648	T7363	T7362	compound	chamber,slides
R4649	T7364	T7362	punct	-,slides
R465	T906	T907	punct	[,7
R4650	T7365	T7344	cc	and,seeded
R4651	T7366	T7344	conj	grown,seeded
R4652	T7367	T7366	advmod	overnight,grown
R4653	T7368	T7366	prep	at,grown
R4654	T7369	T7370	nummod	33,°C
R4655	T7370	T7368	pobj	°C,at
R4656	T7371	T7344	punct	.,seeded
R4657	T7373	T7374	compound	Growth,media
R4658	T7374	T7375	nsubjpass	media,exchanged
R4659	T7376	T7375	auxpass	was,exchanged
R466	T907	T898	parataxis	7,localizes
R4660	T7377	T7375	prep	for,exchanged
R4661	T7378	T7379	amod	fresh,media
R4662	T7379	T7377	pobj	media,for
R4663	T7380	T7379	punct	", ",media
R4664	T7381	T7379	amod	pre-warmed,media
R4665	T7382	T7379	compound	growth,media
R4666	T7383	T7379	acl	containing,media
R4667	T7384	T7385	nummod	500,nM
R4668	T7385	T7386	compound	nM,Lysotracker
R4669	T7386	T7383	dobj	Lysotracker,containing
R467	T908	T909	punct	-,9
R4670	T7387	T7386	cc	or,Lysotracker
R4671	T7388	T7389	nummod	1,mg
R4672	T7389	T7390	nmod	mg,FITC
R4673	T7390	T7386	conj	FITC,Lysotracker
R4674	T7391	T7392	punct	/,ml
R4675	T7392	T7389	prep	ml,mg
R4676	T7393	T7390	compound	dextran,FITC
R4677	T7394	T7390	punct	-,FITC
R4678	T7395	T7375	punct	", ",exchanged
R4679	T7396	T7375	cc	and,exchanged
R468	T909	T907	prep	9,7
R4680	T7397	T7398	nsubjpass	cells,incubated
R4681	T7398	T7375	conj	incubated,exchanged
R4682	T7399	T7398	auxpass	were,incubated
R4683	T7400	T7398	prep	at,incubated
R4684	T7401	T7402	nummod	33,°C
R4685	T7402	T7400	pobj	°C,at
R4686	T7403	T7398	prep	for,incubated
R4687	T7404	T7405	nummod	45,minutes
R4688	T7405	T7403	pobj	minutes,for
R4689	T7406	T7405	cc	or,minutes
R469	T910	T907	punct	],7
R4690	T7407	T7408	nummod	15,minutes
R4691	T7408	T7405	conj	minutes,minutes
R4692	T7409	T7398	punct	", ",incubated
R4693	T7410	T7398	advmod	respectively,incubated
R4694	T7411	T7398	punct	.,incubated
R4695	T7413	T7414	prep	Following,placed
R4696	T7415	T7413	pobj	labeling,Following
R4697	T7416	T7414	punct	", ",placed
R4698	T7417	T7414	nsubjpass	cells,placed
R4699	T7418	T7414	auxpass	were,placed
R47	T224	T222	pobj	activity,of
R470	T911	T873	punct	.,is
R4700	T7419	T7414	advmod	immediately,placed
R4701	T7420	T7414	prep	on,placed
R4702	T7421	T7420	pobj	ice,on
R4703	T7422	T7414	cc	and,placed
R4704	T7423	T7414	conj	washed,placed
R4705	T7424	T7423	prep	for,washed
R4706	T7425	T7426	nummod	10,minutes
R4707	T7426	T7424	pobj	minutes,for
R4708	T7427	T7423	prep	in,washed
R4709	T7428	T7429	npadvmod	ice,cold
R471	T913	T914	compound	Battenin,function
R4710	T7429	T7431	amod	cold,media
R4711	T7430	T7429	punct	-,cold
R4712	T7431	T7427	pobj	media,in
R4713	T7432	T7433	npadvmod	dye,free
R4714	T7433	T7431	amod	free,media
R4715	T7434	T7433	punct	-,free
R4716	T7435	T7423	punct	", ",washed
R4717	T7436	T7423	cc	and,washed
R4718	T7437	T7423	conj	fixed,washed
R4719	T7438	T7437	prep	with,fixed
R472	T914	T915	nsubj	function,remains
R4720	T7439	T7440	nummod	4,%
R4721	T7440	T7441	compound	%,formaldehyde
R4722	T7441	T7438	pobj	formaldehyde,with
R4723	T7442	T7441	prep	in,formaldehyde
R4724	T7443	T7442	pobj	PBS,in
R4725	T7444	T7437	punct	", ",fixed
R4726	T7445	T7437	prep	for,fixed
R4727	T7446	T7447	nummod	20,minutes
R4728	T7447	T7445	pobj	minutes,for
R4729	T7448	T7437	prep	on,fixed
R473	T916	T917	aux	to,elucidated
R4730	T7449	T7448	pobj	ice,on
R4731	T7450	T7414	punct	.,placed
R4732	T7452	T7453	nsubjpass	Chambers,removed
R4733	T7454	T7453	auxpass	were,removed
R4734	T7455	T7453	cc	and,removed
R4735	T7456	T7457	nsubjpass	slides,coverslipped
R4736	T7457	T7453	conj	coverslipped,removed
R4737	T7458	T7457	auxpass	were,coverslipped
R4738	T7459	T7457	prep	with,coverslipped
R4739	T7460	T7461	compound	Vectashield,media
R474	T917	T915	xcomp	elucidated,remains
R4740	T7461	T7459	pobj	media,with
R4741	T7462	T7461	compound	mounting,media
R4742	T7463	T7461	prep	for,media
R4743	T7464	T7465	amod	confocal,microscopy
R4744	T7465	T7466	compound	microscopy,analysis
R4745	T7466	T7463	pobj	analysis,for
R4746	T7467	T7457	punct	", ",coverslipped
R4747	T7468	T7469	mark	as,described
R4748	T7469	T7457	advcl	described,coverslipped
R4749	T7470	T7469	advmod	above,described
R475	T918	T917	auxpass	be,elucidated
R4750	T7471	T7457	punct	.,coverslipped
R4751	T7484	T7485	amod	Morphometric,analysis
R4752	T7486	T7485	prep	of,analysis
R4753	T7487	T7486	pobj	mitochondria,of
R4754	T7489	T7490	compound	TEM,photomicrographs
R4755	T7490	T7491	nsubjpass	photomicrographs,taken
R4756	T7492	T7493	punct	(,magnification
R4757	T7493	T7490	parataxis	magnification,photomicrographs
R4758	T7494	T7495	quantmod	"10,000","40,000"
R4759	T7495	T7493	nummod	"40,000",magnification
R476	T919	T917	punct	", ",elucidated
R4760	T7496	T7494	punct	×,"10,000"
R4761	T7497	T7495	punct	–,"40,000"
R4762	T7498	T7495	punct	×,"40,000"
R4763	T7499	T7493	punct	),magnification
R4764	T7500	T7491	auxpass	were,taken
R4765	T7501	T7491	prep	from,taken
R4766	T7502	T7503	amod	random,fields
R4767	T7503	T7501	pobj	fields,from
R4768	T7504	T7503	compound	grid,fields
R4769	T7505	T7491	punct	.,taken
R477	T920	T921	mark	although,implicated
R4770	T7507	T7508	prep	For,measured
R4771	T7509	T7510	compound	length,measurements
R4772	T7510	T7507	pobj	measurements,For
R4773	T7511	T7508	punct	", ",measured
R4774	T7512	T7513	det	the,side
R4775	T7513	T7508	nsubjpass	side,measured
R4776	T7514	T7513	amod	longest,side
R4777	T7515	T7513	prep	of,side
R4778	T7516	T7517	det	each,mitochondria
R4779	T7517	T7515	pobj	mitochondria,of
R478	T921	T917	advcl	implicated,elucidated
R4780	T7518	T7508	auxpass	was,measured
R4781	T7519	T7508	prep	in,measured
R4782	T7520	T7519	pobj	centimeters,in
R4783	T7521	T7508	punct	", ",measured
R4784	T7522	T7508	cc	and,measured
R4785	T7523	T7524	prep	along,taken
R4786	T7524	T7508	conj	taken,measured
R4787	T7525	T7526	det	the,length
R4788	T7526	T7523	pobj	length,along
R4789	T7527	T7526	prep	of,length
R479	T922	T921	nsubj	studies,implicated
R4790	T7528	T7529	det	the,mitochondria
R4791	T7529	T7527	pobj	mitochondria,of
R4792	T7530	T7524	punct	", ",taken
R4793	T7531	T7532	compound	width,measurements
R4794	T7532	T7524	nsubjpass	measurements,taken
R4795	T7533	T7524	auxpass	were,taken
R4796	T7534	T7535	det	every,mm
R4797	T7535	T7524	npadvmod	mm,taken
R4798	T7536	T7537	quantmod	2.5,4
R4799	T7537	T7535	nummod	4,mm
R48	T225	T221	prep	to,leads
R480	T923	T922	prep	of,studies
R4800	T7538	T7537	punct	–,4
R4801	T7539	T7535	punct	(,mm
R4802	T7540	T7535	amod	dependent,mm
R4803	T7541	T7540	prep	on,dependent
R4804	T7542	T7543	det	the,magnification
R4805	T7543	T7541	pobj	magnification,on
R4806	T7544	T7543	prep	of,magnification
R4807	T7545	T7546	det	the,image
R4808	T7546	T7544	pobj	image,of
R4809	T7547	T7546	compound	micrograph,image
R481	T924	T923	pobj	btn1,of
R4810	T7548	T7524	punct	),taken
R4811	T7549	T7524	punct	.,taken
R4812	T7551	T7552	prep	Following,normalized
R4813	T7553	T7551	pobj	measurement,Following
R4814	T7554	T7552	punct	", ",normalized
R4815	T7555	T7556	det	all,numbers
R4816	T7556	T7552	nsubjpass	numbers,normalized
R4817	T7557	T7552	auxpass	were,normalized
R4818	T7558	T7559	aux	to,reflect
R4819	T7559	T7552	advcl	reflect,normalized
R482	T925	T924	punct	", ",btn1
R4820	T7560	T7561	nummod	one,magnification
R4821	T7561	T7559	dobj	magnification,reflect
R4822	T7562	T7552	cc	and,normalized
R4823	T7563	T7564	nsubjpass	data,analyzed
R4824	T7564	T7552	conj	analyzed,normalized
R4825	T7565	T7564	auxpass	was,analyzed
R4826	T7566	T7564	advcl	using,analyzed
R4827	T7567	T7568	compound	Microsoft,Excel
R4828	T7568	T7569	compound	Excel,software
R4829	T7569	T7566	dobj	software,using
R483	T926	T927	det	the,ortholog
R4830	T7570	T7564	punct	.,analyzed
R4831	T7572	T7573	aux	To,ascertain
R4832	T7573	T7574	advcl	ascertain,converted
R4833	T7575	T7576	amod	unmagnified,size
R4834	T7576	T7573	dobj	size,ascertain
R4835	T7577	T7576	amod	mitochondrial,size
R4836	T7578	T7574	punct	", ",converted
R4837	T7579	T7580	amod	final,data
R4838	T7580	T7574	nsubjpass	data,converted
R4839	T7581	T7580	compound	measurement,data
R484	T927	T924	appos	ortholog,btn1
R4840	T7582	T7580	punct	", ",data
R4841	T7583	T7580	prep	in,data
R4842	T7584	T7583	pobj	centimeters,in
R4843	T7585	T7574	punct	", ",converted
R4844	T7586	T7574	auxpass	was,converted
R4845	T7587	T7574	prep	to,converted
R4846	T7588	T7587	pobj	nanometers,to
R4847	T7589	T7574	prep	according,converted
R4848	T7590	T7589	prep	to,according
R4849	T7591	T7592	compound	scale,bar
R485	T928	T927	compound	yeast,ortholog
R4850	T7592	T7593	compound	bar,representation
R4851	T7593	T7590	pobj	representation,to
R4852	T7594	T7574	punct	.,converted
R4853	T7619	T7620	compound	ATP,measurement
R4854	T7622	T7623	nsubjpass	ATP,measured
R4855	T7624	T7623	auxpass	was,measured
R4856	T7625	T7623	prep	by,measured
R4857	T7626	T7625	pcomp	using,by
R4858	T7627	T7628	det	the,kit
R4859	T7628	T7626	dobj	kit,using
R486	T929	T927	compound	CLN3,ortholog
R4860	T7629	T7630	nmod	CellTiter,GLO®
R4861	T7630	T7628	nmod	GLO®,kit
R4862	T7631	T7630	punct	-,GLO®
R4863	T7632	T7628	amod	Luminescent,kit
R4864	T7633	T7634	compound	Cell,Viability
R4865	T7634	T7628	compound	Viability,kit
R4866	T7635	T7636	punct	(,Promega
R4867	T7636	T7628	parataxis	Promega,kit
R4868	T7637	T7636	punct	),Promega
R4869	T7638	T7623	punct	", ",measured
R487	T930	T921	punct	", ",implicated
R4870	T7639	T7623	prep	according,measured
R4871	T7640	T7639	prep	to,according
R4872	T7641	T7642	det	the,manufacturer
R4873	T7642	T7643	poss	manufacturer,recommendations
R4874	T7643	T7640	pobj	recommendations,to
R4875	T7644	T7642	case	's,manufacturer
R4876	T7645	T7623	punct	.,measured
R4877	T7647	T7648	advmod	Briefly,plated
R4878	T7649	T7648	punct	", ",plated
R4879	T7650	T7648	nsubjpass	cells,plated
R488	T931	T921	aux	have,implicated
R4880	T7651	T7648	auxpass	were,plated
R4881	T7652	T7648	prep	in,plated
R4882	T7653	T7654	det	a,plate
R4883	T7654	T7652	pobj	plate,in
R4884	T7655	T7654	amod	black,plate
R4885	T7656	T7657	amod	opaque,walled
R4886	T7657	T7654	amod	walled,plate
R4887	T7658	T7657	punct	-,walled
R4888	T7659	T7660	nummod	96,well
R4889	T7660	T7654	compound	well,plate
R489	T932	T921	dobj	battenin,implicated
R4890	T7661	T7662	punct	(,Bioscience
R4891	T7662	T7654	parataxis	Bioscience,plate
R4892	T7663	T7662	compound	Packard,Bioscience
R4893	T7664	T7662	punct	),Bioscience
R4894	T7665	T7648	prep	at,plated
R4895	T7666	T7667	det	a,density
R4896	T7667	T7665	pobj	density,at
R4897	T7668	T7667	prep	of,density
R4898	T7669	T7668	pobj	20000,of
R4899	T7670	T7671	punct	/,well
R49	T226	T225	pobj	deposition,to
R490	T933	T921	prep	in,implicated
R4900	T7671	T7669	prep	well,20000
R4901	T7672	T7648	cc	and,plated
R4902	T7673	T7648	conj	incubated,plated
R4903	T7674	T7673	prep	at,incubated
R4904	T7675	T7676	nummod	33,°C
R4905	T7676	T7674	pobj	°C,at
R4906	T7677	T7673	advmod	overnight,incubated
R4907	T7678	T7648	punct	.,plated
R4908	T7680	T7681	det	The,day
R4909	T7681	T7683	npadvmod	day,added
R491	T934	T935	amod	lysosomal,homeostasis
R4910	T7682	T7681	amod	following,day
R4911	T7684	T7683	punct	", ",added
R4912	T7685	T7686	compound	CellTiter,GLO®
R4913	T7686	T7688	compound	GLO®,Reagent
R4914	T7687	T7686	punct	-,GLO®
R4915	T7688	T7683	nsubjpass	Reagent,added
R4916	T7689	T7683	auxpass	was,added
R4917	T7690	T7683	prep	to,added
R4918	T7691	T7692	det	each,well
R4919	T7692	T7690	pobj	well,to
R492	T935	T933	pobj	homeostasis,in
R4920	T7693	T7683	cc	and,added
R4921	T7694	T7695	compound	cell,lysis
R4922	T7695	T7696	nsubjpass	lysis,induced
R4923	T7696	T7683	conj	induced,added
R4924	T7697	T7696	auxpass	was,induced
R4925	T7698	T7696	prep	by,induced
R4926	T7699	T7698	pcomp	mixing,by
R4927	T7700	T7701	nummod	2,minutes
R4928	T7701	T7699	npadvmod	minutes,mixing
R4929	T7702	T7696	punct	.,induced
R493	T936	T935	compound	pH,homeostasis
R4930	T7704	T7705	det	An,curve
R4931	T7705	T7708	nsubjpass	curve,prepared
R4932	T7706	T7705	nmod	ATP,curve
R4933	T7707	T7705	amod	standard,curve
R4934	T7709	T7708	auxpass	was,prepared
R4935	T7710	T7708	prep	in,prepared
R4936	T7711	T7712	det	the,plate
R4937	T7712	T7710	pobj	plate,in
R4938	T7713	T7712	amod	same,plate
R4939	T7714	T7708	punct	.,prepared
R494	T937	T935	cc	and,homeostasis
R4940	T7716	T7717	prep	Before,adapted
R4941	T7718	T7716	pcomp	recording,Before
R4942	T7719	T7718	dobj	luminescence,recording
R4943	T7720	T7718	prep	with,recording
R4944	T7721	T7722	det	a,luminometer
R4945	T7722	T7720	pobj	luminometer,with
R4946	T7723	T7722	compound	microplate,luminometer
R4947	T7724	T7722	punct	(,luminometer
R4948	T7725	T7726	compound	MicroLumat,96V
R4949	T7726	T7722	appos	96V,luminometer
R495	T938	T939	compound	amino,acid
R4950	T7727	T7726	compound	Plus,96V
R4951	T7728	T7726	compound	LB,96V
R4952	T7729	T7730	punct	", ",Techonologies
R4953	T7730	T7722	parataxis	Techonologies,luminometer
R4954	T7731	T7730	compound	Berthold,Techonologies
R4955	T7732	T7730	punct	),Techonologies
R4956	T7733	T7717	punct	", ",adapted
R4957	T7734	T7735	det	the,plate
R4958	T7735	T7717	nsubjpass	plate,adapted
R4959	T7736	T7717	auxpass	was,adapted
R496	T939	T940	compound	acid,transport
R4960	T7737	T7717	dep	dark,adapted
R4961	T7738	T7717	prep	for,adapted
R4962	T7739	T7740	nummod	10,minutes
R4963	T7740	T7738	pobj	minutes,for
R4964	T7741	T7717	prep	at,adapted
R4965	T7742	T7743	compound	room,temperature
R4966	T7743	T7741	pobj	temperature,at
R4967	T7744	T7745	aux	to,stabilize
R4968	T7745	T7717	advcl	stabilize,adapted
R4969	T7746	T7747	det	the,signal
R497	T940	T935	conj	transport,homeostasis
R4970	T7747	T7745	dobj	signal,stabilize
R4971	T7748	T7747	compound	luminescence,signal
R4972	T7749	T7717	punct	.,adapted
R4973	T7780	T7781	compound	Hydrogen,peroxide
R4974	T7781	T7782	compound	peroxide,treatment
R4975	T7782	T7783	compound	treatment,assay
R4976	T7785	T7786	nsubjpass	Cells,plated
R4977	T7787	T7786	auxpass	were,plated
R4978	T7788	T7786	prep	at,plated
R4979	T7789	T7790	det	a,density
R498	T941	T942	punct	[,11
R4980	T7790	T7788	pobj	density,at
R4981	T7791	T7790	prep	of,density
R4982	T7792	T7793	nummod	"10,000",cells
R4983	T7793	T7791	pobj	cells,of
R4984	T7794	T7795	punct	/,well
R4985	T7795	T7793	prep	well,cells
R4986	T7796	T7786	prep	in,plated
R4987	T7797	T7798	nummod	96,well
R4988	T7798	T7800	compound	well,plates
R4989	T7799	T7798	punct	-,well
R499	T942	T921	parataxis	11,implicated
R4990	T7800	T7796	pobj	plates,in
R4991	T7801	T7786	cc	and,plated
R4992	T7802	T7786	conj	incubated,plated
R4993	T7803	T7802	prep	at,incubated
R4994	T7804	T7805	nummod	33,°C
R4995	T7805	T7803	pobj	°C,at
R4996	T7806	T7802	advmod	overnight,incubated
R4997	T7807	T7786	punct	.,plated
R4998	T7809	T7810	det	The,day
R4999	T7810	T7812	npadvmod	day,dispersed
R5	T174	T171	cc	and,trafficking
R50	T227	T226	prep	of,deposition
R500	T943	T942	nummod	10,11
R5000	T7811	T7810	amod	following,day
R5001	T7813	T7812	punct	", ",dispersed
R5002	T7814	T7815	amod	fresh,media
R5003	T7815	T7812	nsubjpass	media,dispersed
R5004	T7816	T7815	acl	containing,media
R5005	T7817	T7818	amod	varying,concentrations
R5006	T7818	T7816	dobj	concentrations,containing
R5007	T7819	T7818	prep	of,concentrations
R5008	T7820	T7821	compound	hydrogen,peroxide
R5009	T7821	T7819	pobj	peroxide,of
R501	T944	T942	punct	",",11
R5010	T7822	T7812	auxpass	was,dispersed
R5011	T7823	T7812	prep	to,dispersed
R5012	T7824	T7825	det	each,well
R5013	T7825	T7823	pobj	well,to
R5014	T7826	T7812	punct	.,dispersed
R5015	T7828	T7829	nsubjpass	Cells,incubated
R5016	T7830	T7829	auxpass	were,incubated
R5017	T7831	T7829	prep	in,incubated
R5018	T7832	T7833	det	the,presence
R5019	T7833	T7831	pobj	presence,in
R502	T945	T942	punct	],11
R5020	T7834	T7833	prep	of,presence
R5021	T7835	T7836	compound	hydrogen,peroxide
R5022	T7836	T7834	pobj	peroxide,of
R5023	T7837	T7829	prep	for,incubated
R5024	T7838	T7839	nummod	24,hours
R5025	T7839	T7837	pobj	hours,for
R5026	T7840	T7829	punct	", ",incubated
R5027	T7841	T7829	prep	at,incubated
R5028	T7842	T7843	nummod	33,°C
R5029	T7843	T7841	pobj	°C,at
R503	T946	T915	punct	.,remains
R5030	T7844	T7829	punct	", ",incubated
R5031	T7845	T7829	cc	and,incubated
R5032	T7846	T7847	nsubjpass	viability,measured
R5033	T7847	T7829	conj	measured,incubated
R5034	T7848	T7847	auxpass	was,measured
R5035	T7849	T7847	advcl	using,measured
R5036	T7850	T7851	det	the,Assay
R5037	T7851	T7849	dobj	Assay,using
R5038	T7852	T7853	nmod	CellTiter,96®
R5039	T7853	T7851	nmod	96®,Assay
R504	T948	T949	aux	To,explore
R5040	T7854	T7853	punct	-,96®
R5041	T7855	T7851	amod	AQueous,Assay
R5042	T7856	T7851	amod	Non-Radioactive,Assay
R5043	T7857	T7851	compound	Cell,Assay
R5044	T7858	T7851	compound	Proliferation,Assay
R5045	T7859	T7860	punct	(,Promega
R5046	T7860	T7851	parataxis	Promega,Assay
R5047	T7861	T7860	punct	),Promega
R5048	T7862	T7849	punct	", ",using
R5049	T7863	T7849	prep	according,using
R505	T949	T950	advcl	explore,generated
R5050	T7864	T7863	prep	to,according
R5051	T7865	T7866	det	the,manufacturer
R5052	T7866	T7867	poss	manufacturer,specifications
R5053	T7867	T7864	pobj	specifications,to
R5054	T7868	T7866	case	's,manufacturer
R5055	T7869	T7847	punct	.,measured
R5056	T7982	T7983	amod	Neuronal,marker
R5057	T7983	T7984	compound	marker,expression
R5058	T7984	T7985	dep	expression,shown
R5059	T7986	T7984	prep	in,expression
R506	T951	T952	compound	JNCL,pathogenesis
R5060	T7987	T7988	nmod	CbCln3,cells
R5061	T7988	T7986	pobj	cells,in
R5062	T7989	T7987	punct	+,CbCln3
R5063	T7990	T7987	punct	/,CbCln3
R5064	T7991	T7987	punct	+,CbCln3
R5065	T7992	T7985	nsubjpass	Characterization,shown
R5066	T7993	T7992	prep	of,Characterization
R5067	T7994	T7995	nmod	CbCln3,cells
R5068	T7995	T7993	pobj	cells,of
R5069	T7996	T7994	punct	+,CbCln3
R507	T952	T949	dobj	pathogenesis,explore
R5070	T7997	T7994	punct	/,CbCln3
R5071	T7998	T7994	punct	+,CbCln3
R5072	T7999	T7992	prep	by,Characterization
R5073	T8000	T7999	pobj	immunofluorescence,by
R5074	T8001	T8000	prep	with,immunofluorescence
R5075	T8002	T8003	compound	marker,antibodies
R5076	T8003	T8001	pobj	antibodies,with
R5077	T8004	T7985	auxpass	is,shown
R5078	T8005	T7985	punct	.,shown
R5079	T8007	T8008	nmod	CbCln3,precursors
R508	T953	T952	cc	and,pathogenesis
R5080	T8008	T8012	nsubj	precursors,exhibit
R5081	T8009	T8007	punct	+,CbCln3
R5082	T8010	T8007	punct	/,CbCln3
R5083	T8011	T8007	punct	+,CbCln3
R5084	T8013	T8014	compound	nestin,expression
R5085	T8014	T8012	dobj	expression,exhibit
R5086	T8015	T8016	punct	(,a
R5087	T8016	T8014	parataxis	a,expression
R5088	T8017	T8016	punct	),a
R5089	T8018	T8014	cc	but,expression
R509	T954	T955	compound	battenin,function
R5090	T8019	T8018	neg	not,but
R5091	T8020	T8021	compound	GFAP,expression
R5092	T8021	T8014	conj	expression,expression
R5093	T8022	T8023	punct	(,b
R5094	T8023	T8021	parataxis	b,expression
R5095	T8024	T8023	punct	),b
R5096	T8025	T8012	punct	", ",exhibit
R5097	T8026	T8012	advcl	consistent,exhibit
R5098	T8027	T8026	prep	with,consistent
R5099	T8028	T8029	det	a,identity
R51	T228	T229	amod	mitochondrial,synthase
R510	T955	T952	conj	function,pathogenesis
R5100	T8029	T8027	pobj	identity,with
R5101	T8030	T8029	amod	neuronal,identity
R5102	T8031	T8029	compound	precursor,identity
R5103	T8032	T8012	punct	.,exhibit
R5104	T8034	T8035	prep	Upon,achieved
R5105	T8036	T8034	pobj	stimulation,Upon
R5106	T8037	T8036	prep	with,stimulation
R5107	T8038	T8039	det	a,cocktail
R5108	T8039	T8037	pobj	cocktail,with
R5109	T8040	T8039	compound	differentiation,cocktail
R511	T956	T950	punct	", ",generated
R5110	T8041	T8042	punct	(,see
R5111	T8042	T8036	parataxis	see,stimulation
R5112	T8043	T8042	dobj	Methods,see
R5113	T8044	T8042	punct	),see
R5114	T8045	T8035	punct	", ",achieved
R5115	T8046	T8047	nmod	CbCln3,cells
R5116	T8047	T8035	nsubj	cells,achieved
R5117	T8048	T8046	punct	+,CbCln3
R5118	T8049	T8046	punct	/,CbCln3
R5119	T8050	T8046	punct	+,CbCln3
R512	T957	T950	nsubj	we,generated
R5120	T8051	T8052	npadvmod	neuron,like
R5121	T8052	T8054	amod	like,morphology
R5122	T8053	T8052	punct	-,like
R5123	T8054	T8035	dobj	morphology,achieved
R5124	T8055	T8035	punct	", ",achieved
R5125	T8056	T8035	prep	with,achieved
R5126	T8057	T8058	amod	rounded,bodies
R5127	T8058	T8056	pobj	bodies,with
R5128	T8059	T8058	compound	cell,bodies
R5129	T8060	T8058	cc	and,bodies
R513	T958	T950	advmod	previously,generated
R5130	T8061	T8058	conj	extension,bodies
R5131	T8062	T8061	prep	of,extension
R5132	T8063	T8062	pobj	processes,of
R5133	T8064	T8035	punct	", ",achieved
R5134	T8065	T8035	cc	and,achieved
R5135	T8066	T8067	nmod	MAP2,expression
R5136	T8067	T8076	nsubjpass	expression,increased
R5137	T8068	T8069	punct	(,c
R5138	T8069	T8066	parataxis	c,MAP2
R5139	T8070	T8069	punct	),c
R514	T959	T960	det	a,model
R5140	T8071	T8066	cc	and,MAP2
R5141	T8072	T8066	conj	NeuN,MAP2
R5142	T8073	T8074	punct	(,d
R5143	T8074	T8072	parataxis	d,NeuN
R5144	T8075	T8074	punct	),d
R5145	T8076	T8035	conj	increased,achieved
R5146	T8077	T8076	auxpass	was,increased
R5147	T8078	T8076	punct	.,increased
R5148	T8080	T8081	nmod	CbCln3,cells
R5149	T8081	T8085	nsubj	cells,are
R515	T960	T950	dobj	model,generated
R5150	T8082	T8080	punct	+,CbCln3
R5151	T8083	T8080	punct	/,CbCln3
R5152	T8084	T8080	punct	+,CbCln3
R5153	T8086	T8085	acomp	negative,are
R5154	T8087	T8086	prep	for,negative
R5155	T8088	T8089	det	the,marker
R5156	T8089	T8087	pobj	marker,for
R5157	T8090	T8089	compound	Purkinje,marker
R5158	T8091	T8089	compound	neuron,marker
R5159	T8092	T8089	appos	calbindin,marker
R516	T961	T962	advmod	genetically,precise
R5160	T8093	T8094	punct	(,e
R5161	T8094	T8089	parataxis	e,marker
R5162	T8095	T8094	punct	),e
R5163	T8096	T8085	punct	.,are
R5164	T8098	T8099	nmod	CbCln3,Δex7
R5165	T8099	T8102	nmod	Δex7,lines
R5166	T8100	T8099	punct	+,Δex7
R5167	T8101	T8099	punct	/,Δex7
R5168	T8102	T8114	nsubj	lines,exhibited
R5169	T8103	T8099	punct	/,Δex7
R517	T962	T960	amod	precise,model
R5170	T8104	T8099	nummod	8,Δex7
R5171	T8105	T8099	cc	and,Δex7
R5172	T8106	T8107	nmod	CbCln3Δex7,Δex7
R5173	T8107	T8099	conj	Δex7,Δex7
R5174	T8108	T8107	punct	/,Δex7
R5175	T8109	T8107	nummod	8,Δex7
R5176	T8110	T8107	punct	/,Δex7
R5177	T8111	T8107	punct	/,Δex7
R5178	T8112	T8107	nummod	8,Δex7
R5179	T8113	T8102	compound	cell,lines
R518	T963	T960	compound	JNCL,model
R5180	T8115	T8116	amod	identical,results
R5181	T8116	T8114	dobj	results,exhibited
R5182	T8117	T8118	compound	marker,immunofluorescence
R5183	T8118	T8116	compound	immunofluorescence,results
R5184	T8119	T8114	punct	.,exhibited
R5185	T8121	T8122	meta	a,magnification
R5186	T8123	T8121	punct	", ",a
R5187	T8124	T8121	dep	b,a
R5188	T8125	T8121	punct	),a
R5189	T8126	T8122	nummod	20,magnification
R519	T964	T960	compound	mouse,model
R5190	T8127	T8126	punct	×,20
R5191	T8128	T8122	punct	;,magnification
R5192	T8129	T8130	meta	c,magnification
R5193	T8130	T8122	appos	magnification,magnification
R5194	T8131	T8129	punct	", ",c
R5195	T8132	T8129	dep	d,c
R5196	T8133	T8129	punct	", ",c
R5197	T8134	T8129	dep	e,c
R5198	T8135	T8129	punct	),c
R5199	T8136	T8130	nummod	40,magnification
R52	T229	T227	pobj	synthase,of
R520	T965	T950	punct	.,generated
R5200	T8137	T8136	punct	×,40
R5201	T8138	T8122	punct	.,magnification
R5202	T8215	T8216	compound	RT,PCR
R5203	T8216	T8218	dep	PCR,shown
R5204	T8217	T8216	punct	-,PCR
R5205	T8219	T8216	prep	of,PCR
R5206	T8220	T8221	compound	Cln3,mRNA
R5207	T8221	T8219	pobj	mRNA,of
R5208	T8222	T8216	prep	in,PCR
R5209	T8223	T8224	amod	wild,type
R521	T967	T968	nmod	Cln3Δex7,mice
R5210	T8224	T8226	nmod	type,cells
R5211	T8225	T8224	punct	-,type
R5212	T8226	T8222	pobj	cells,in
R5213	T8227	T8224	cc	and,type
R5214	T8228	T8224	conj	homozygous,type
R5215	T8229	T8226	nmod	CbCln3Δex7,cells
R5216	T8230	T8229	punct	/,CbCln3Δex7
R5217	T8231	T8229	nummod	8,CbCln3Δex7
R5218	T8232	T8233	nmod	Cln3,products
R5219	T8233	T8218	nsubjpass	products,shown
R522	T968	T974	nsubj	mice,harbor
R5220	T8234	T8235	npadvmod	Exon1,forward
R5221	T8235	T8237	amod	forward,reverse
R5222	T8236	T8235	punct	-,forward
R5223	T8237	T8233	nmod	reverse,products
R5224	T8238	T8237	punct	", ",reverse
R5225	T8239	T8237	npadvmod	Exon,reverse
R5226	T8240	T8239	nummod	15,Exon
R5227	T8241	T8237	punct	-,reverse
R5228	T8242	T8243	compound	RT,PCR
R5229	T8243	T8233	compound	PCR,products
R523	T969	T967	punct	/,Cln3Δex7
R5230	T8244	T8243	punct	-,PCR
R5231	T8245	T8218	auxpass	are,shown
R5232	T8246	T8218	punct	", ",shown
R5233	T8247	T8218	prep	from,shown
R5234	T8248	T8249	amod	total,RNA
R5235	T8249	T8247	pobj	RNA,from
R5236	T8250	T8251	amod	wild,type
R5237	T8251	T8249	nmod	type,RNA
R5238	T8252	T8251	punct	-,type
R5239	T8253	T8251	punct	(,type
R524	T970	T967	nummod	8,Cln3Δex7
R5240	T8254	T8255	punct	+,+
R5241	T8255	T8251	appos	+,type
R5242	T8256	T8255	punct	/,+
R5243	T8257	T8251	punct	),type
R5244	T8258	T8251	cc	or,type
R5245	T8259	T8260	amod	homozygous,mutant
R5246	T8260	T8251	conj	mutant,type
R5247	T8261	T8260	punct	(,mutant
R5248	T8262	T8260	appos	Δex7,mutant
R5249	T8263	T8262	punct	/,Δex7
R525	T971	T968	amod	knock,mice
R5250	T8264	T8262	nummod	8,Δex7
R5251	T8265	T8262	punct	/,Δex7
R5252	T8266	T8262	appos	Δex7,Δex7
R5253	T8267	T8266	punct	/,Δex7
R5254	T8268	T8266	nummod	8,Δex7
R5255	T8269	T8249	punct	),RNA
R5256	T8270	T8249	nmod	brain,RNA
R5257	T8271	T8270	cc	and,brain
R5258	T8272	T8273	compound	cell,line
R5259	T8273	T8270	conj	line,brain
R526	T972	T971	punct	-,knock
R5260	T8274	T8218	punct	.,shown
R5261	T8276	T8277	nmod	Brain,products
R5262	T8277	T8285	nsubj	products,had
R5263	T8278	T8276	cc	and,Brain
R5264	T8279	T8280	compound	cell,line
R5265	T8280	T8276	conj	line,Brain
R5266	T8281	T8282	compound	RT,PCR
R5267	T8282	T8277	compound	PCR,products
R5268	T8283	T8282	punct	-,PCR
R5269	T8284	T8277	compound	reaction,products
R527	T973	T971	prt	in,knock
R5270	T8286	T8287	amod	identical,patterns
R5271	T8287	T8285	dobj	patterns,had
R5272	T8288	T8287	compound	band,patterns
R5273	T8289	T8285	prep	on,had
R5274	T8290	T8291	compound	ethidium,bromide
R5275	T8291	T8293	npadvmod	bromide,stained
R5276	T8292	T8291	punct	-,bromide
R5277	T8293	T8294	amod	stained,gels
R5278	T8294	T8289	pobj	gels,on
R5279	T8295	T8294	compound	agarose,gels
R528	T975	T976	det	the,mutation
R5280	T8296	T8285	punct	.,had
R5281	T8298	T8299	amod	Wild,type
R5282	T8299	T8301	compound	type,product
R5283	T8300	T8299	punct	-,type
R5284	T8301	T8305	nsubj	product,was
R5285	T8302	T8303	compound	RT,PCR
R5286	T8303	T8301	compound	PCR,product
R5287	T8304	T8303	punct	-,PCR
R5288	T8306	T8307	det	a,band
R5289	T8307	T8305	attr	band,was
R529	T976	T974	dobj	mutation,harbor
R5290	T8308	T8307	amod	single,band
R5291	T8309	T8310	punct	~,1.6
R5292	T8310	T8311	nummod	1.6,kb
R5293	T8311	T8307	compound	kb,band
R5294	T8312	T8305	cc	and,was
R5295	T8313	T8314	compound	mutant,products
R5296	T8314	T8315	nsubj	products,were
R5297	T8315	T8305	conj	were,was
R5298	T8316	T8317	punct	~,1.6
R5299	T8317	T8318	nummod	1.6,kb
R53	T230	T229	compound	ATP,synthase
R530	T977	T978	punct	~,1
R5300	T8318	T8315	attr	kb,were
R5301	T8319	T8317	punct	", ",1.6
R5302	T8320	T8321	punct	~,1.5
R5303	T8321	T8317	conj	1.5,1.6
R5304	T8322	T8321	punct	", ",1.5
R5305	T8323	T8324	punct	~,1.4
R5306	T8324	T8321	conj	1.4,1.5
R5307	T8325	T8324	punct	", ",1.4
R5308	T8326	T8327	punct	~,1.35
R5309	T8327	T8324	conj	1.35,1.4
R531	T978	T979	nummod	1,kb
R5310	T8328	T8327	punct	", ",1.35
R5311	T8329	T8327	cc	and,1.35
R5312	T8330	T8331	punct	~,1.3
R5313	T8331	T8327	conj	1.3,1.35
R5314	T8332	T8315	punct	", ",were
R5315	T8333	T8315	advcl	representing,were
R5316	T8334	T8335	amod	multiple,variants
R5317	T8335	T8333	dobj	variants,representing
R5318	T8336	T8337	compound	mutant,splice
R5319	T8337	T8335	compound	splice,variants
R532	T979	T976	nmod	kb,mutation
R5320	T8338	T8315	punct	.,were
R5321	T8445	T8446	nmod	Battenin,marker
R5322	T8446	T8451	compound	marker,co-staining
R5323	T8447	T8445	cc	and,Battenin
R5324	T8448	T8445	conj	lysosomal,Battenin
R5325	T8449	T8448	cc	and,lysosomal
R5326	T8450	T8448	conj	endosomal,lysosomal
R5327	T8451	T8452	dep	co-staining,shown
R5328	T8453	T8451	prep	in,co-staining
R5329	T8454	T8455	amod	wild,type
R533	T980	T976	amod	common,mutation
R5330	T8455	T8457	nmod	type,cells
R5331	T8456	T8455	punct	-,type
R5332	T8457	T8453	pobj	cells,in
R5333	T8458	T8455	cc	and,type
R5334	T8459	T8455	conj	homozygous,type
R5335	T8460	T8461	nmod	CbCln3Δex7,precursor
R5336	T8461	T8457	compound	precursor,cells
R5337	T8462	T8460	punct	/,CbCln3Δex7
R5338	T8463	T8460	nummod	8,CbCln3Δex7
R5339	T8464	T8461	amod	cerebellar,precursor
R534	T981	T976	compound	JNCL,mutation
R5340	T8465	T8466	compound	Batp1,immunostaining
R5341	T8466	T8452	nsubjpass	immunostaining,shown
R5342	T8467	T8466	prep	of,immunostaining
R5343	T8468	T8469	amod	wild,type
R5344	T8469	T8467	pobj	type,of
R5345	T8470	T8469	punct	-,type
R5346	T8471	T8469	punct	(,type
R5347	T8472	T8469	appos	CbCln3,type
R5348	T8473	T8472	punct	+,CbCln3
R5349	T8474	T8472	punct	/,CbCln3
R535	T982	T974	cc	and,harbor
R5350	T8475	T8472	punct	+,CbCln3
R5351	T8476	T8469	punct	),type
R5352	T8477	T8469	cc	and,type
R5353	T8478	T8479	amod	homozygous,mutant
R5354	T8479	T8480	nmod	mutant,cells
R5355	T8480	T8469	conj	cells,type
R5356	T8481	T8479	punct	(,mutant
R5357	T8482	T8483	nmod	CbCln3Δex7,Δex7
R5358	T8483	T8479	appos	Δex7,mutant
R5359	T8484	T8483	punct	/,Δex7
R536	T983	T974	conj	express,harbor
R5360	T8485	T8483	nummod	8,Δex7
R5361	T8486	T8483	punct	/,Δex7
R5362	T8487	T8483	punct	/,Δex7
R5363	T8488	T8483	nummod	8,Δex7
R5364	T8489	T8480	punct	),cells
R5365	T8490	T8480	amod	cerebellar,cells
R5366	T8491	T8480	compound	precursor,cells
R5367	T8492	T8452	auxpass	is,shown
R5368	T8493	T8452	punct	", ",shown
R5369	T8494	T8452	prep	with,shown
R537	T984	T985	det	a,isoform
R5370	T8495	T8494	pobj	co-staining,with
R5371	T8496	T8495	prep	for,co-staining
R5372	T8497	T8496	pobj	lysosomes,for
R5373	T8498	T8497	punct	(,lysosomes
R5374	T8499	T8497	appos	Lamp,lysosomes
R5375	T8500	T8499	nummod	1,Lamp
R5376	T8501	T8497	punct	),lysosomes
R5377	T8502	T8497	punct	", ",lysosomes
R5378	T8503	T8504	amod	early,endosomes
R5379	T8504	T8497	conj	endosomes,lysosomes
R538	T985	T983	dobj	isoform,express
R5380	T8505	T8504	punct	(,endosomes
R5381	T8506	T8504	appos	EEA1,endosomes
R5382	T8507	T8504	punct	),endosomes
R5383	T8508	T8504	punct	", ",endosomes
R5384	T8509	T8504	cc	and,endosomes
R5385	T8510	T8511	amod	late,endosomes
R5386	T8511	T8504	conj	endosomes,endosomes
R5387	T8512	T8511	punct	(,endosomes
R5388	T8513	T8511	appos	Rab7,endosomes
R5389	T8514	T8452	punct	),shown
R539	T986	T985	amod	non-truncated,isoform
R5390	T8515	T8452	punct	.,shown
R5391	T8517	T8518	amod	Significant,overlap
R5392	T8518	T8519	nsubjpass	overlap,seen
R5393	T8520	T8518	prep	of,overlap
R5394	T8521	T8522	compound	Batp1,signal
R5395	T8522	T8520	pobj	signal,of
R5396	T8523	T8524	punct	(,red
R5397	T8524	T8522	parataxis	red,signal
R5398	T8525	T8524	punct	),red
R5399	T8526	T8518	prep	with,overlap
R54	T231	T229	punct	", ",synthase
R540	T987	T988	compound	mutant,battenin
R5400	T8527	T8526	pobj	EEA1,with
R5401	T8528	T8529	punct	(,panels
R5402	T8529	T8527	parataxis	panels,EEA1
R5403	T8530	T8529	amod	green,panels
R5404	T8531	T8529	punct	", ",panels
R5405	T8532	T8529	amod	middle,panels
R5406	T8533	T8529	punct	),panels
R5407	T8534	T8527	cc	and,EEA1
R5408	T8535	T8527	conj	Rab7,EEA1
R5409	T8536	T8537	punct	(,panels
R541	T988	T985	compound	battenin,isoform
R5410	T8537	T8535	parataxis	panels,Rab7
R5411	T8538	T8537	amod	green,panels
R5412	T8539	T8537	punct	", ",panels
R5413	T8540	T8537	amod	bottom,panels
R5414	T8541	T8537	punct	),panels
R5415	T8542	T8519	aux	can,seen
R5416	T8543	T8519	auxpass	be,seen
R5417	T8544	T8519	prep	as,seen
R5418	T8545	T8544	amod	yellow,as
R5419	T8546	T8547	advmod	when,merged
R542	T989	T990	dep	that,is
R5420	T8547	T8519	advcl	merged,seen
R5421	T8548	T8549	det	the,channels
R5422	T8549	T8547	nsubjpass	channels,merged
R5423	T8550	T8549	nummod	two,channels
R5424	T8551	T8547	auxpass	are,merged
R5425	T8552	T8553	punct	(,Merge
R5426	T8553	T8547	parataxis	Merge,merged
R5427	T8554	T8553	punct	),Merge
R5428	T8555	T8519	punct	.,seen
R5429	T8557	T8558	det	The,degree
R543	T990	T985	relcl	is,isoform
R5430	T8558	T8559	nsubj	degree,is
R5431	T8560	T8558	prep	of,degree
R5432	T8561	T8562	compound	Batp1,overlap
R5433	T8562	T8560	pobj	overlap,of
R5434	T8563	T8559	acomp	greatest,is
R5435	T8564	T8559	prep	with,is
R5436	T8565	T8564	pobj	Rab7,with
R5437	T8566	T8559	punct	.,is
R5438	T8568	T8569	advmod	Only,overlap
R5439	T8569	T8571	nsubjpass	overlap,seen
R544	T991	T990	acomp	detectable,is
R5440	T8570	T8569	amod	limited,overlap
R5441	T8572	T8569	prep	between,overlap
R5442	T8573	T8572	pobj	Batp1,between
R5443	T8574	T8575	punct	(,red
R5444	T8575	T8573	parataxis	red,Batp1
R5445	T8576	T8575	punct	),red
R5446	T8577	T8573	cc	and,Batp1
R5447	T8578	T8573	conj	Lamp,Batp1
R5448	T8579	T8578	nummod	1,Lamp
R5449	T8580	T8581	punct	(,panels
R545	T992	T990	prep	with,is
R5450	T8581	T8578	parataxis	panels,Lamp
R5451	T8582	T8581	amod	green,panels
R5452	T8583	T8581	punct	", ",panels
R5453	T8584	T8581	amod	top,panels
R5454	T8585	T8581	punct	),panels
R5455	T8586	T8571	aux	can,seen
R5456	T8587	T8571	auxpass	be,seen
R5457	T8588	T8571	punct	.,seen
R5458	T8590	T8591	compound	Batp1,signal
R5459	T8591	T8592	nsubj	signal,is
R546	T993	T992	pobj	antibodies,with
R5460	T8593	T8591	prep	in,signal
R5461	T8594	T8595	amod	homozygous,cells
R5462	T8595	T8593	pobj	cells,in
R5463	T8596	T8595	nmod	CbCln3Δex7,cells
R5464	T8597	T8596	punct	/,CbCln3Δex7
R5465	T8598	T8596	nummod	8,CbCln3Δex7
R5466	T8599	T8600	advmod	significantly,reduced
R5467	T8600	T8592	acomp	reduced,is
R5468	T8601	T8592	punct	", ",is
R5469	T8602	T8592	cc	but,is
R547	T994	T993	acl	recognizing,antibodies
R5470	T8603	T8604	amod	significant,overlap
R5471	T8604	T8605	nsubjpass	overlap,seen
R5472	T8605	T8592	conj	seen,is
R5473	T8606	T8604	prep	with,overlap
R5474	T8607	T8606	pobj	EEA1,with
R5475	T8608	T8607	cc	and,EEA1
R5476	T8609	T8607	conj	Rab7,EEA1
R5477	T8610	T8604	punct	", ",overlap
R5478	T8611	T8604	cc	and,overlap
R5479	T8612	T8613	advmod	very,little
R548	T995	T996	npadvmod	C,terminal
R5480	T8613	T8614	amod	little,overlap
R5481	T8614	T8604	conj	overlap,overlap
R5482	T8615	T8614	nmod	Lamp,overlap
R5483	T8616	T8615	nummod	1,Lamp
R5484	T8617	T8605	punct	", ",seen
R5485	T8618	T8605	aux	can,seen
R5486	T8619	T8605	auxpass	be,seen
R5487	T8620	T8605	prep	as,seen
R5488	T8621	T8620	amod	yellow,as
R5489	T8622	T8605	prep	in,seen
R549	T996	T998	amod	terminal,epitopes
R5490	T8623	T8624	det	the,panels
R5491	T8624	T8622	pobj	panels,in
R5492	T8625	T8624	amod	respective,panels
R5493	T8626	T8624	amod	merged,panels
R5494	T8627	T8605	punct	.,seen
R5495	T8629	T8630	advmod	Notably,appear
R5496	T8631	T8630	punct	", ",appear
R5497	T8632	T8633	nmod	Lamp,localization
R5498	T8633	T8630	nsubj	localization,appear
R5499	T8634	T8632	nummod	1,Lamp
R55	T232	T233	compound	subunit,c
R550	T997	T996	punct	-,terminal
R5500	T8635	T8632	cc	and,Lamp
R5501	T8636	T8632	conj	EEA1,Lamp
R5502	T8637	T8630	oprd	altered,appear
R5503	T8638	T8630	punct	", ",appear
R5504	T8639	T8630	cc	and,appear
R5505	T8640	T8641	compound	Rab7,staining
R5506	T8641	T8642	nsubj	staining,was
R5507	T8642	T8630	conj	was,appear
R5508	T8643	T8642	advmod	frequently,was
R5509	T8644	T8645	advmod	less,intense
R551	T998	T994	dobj	epitopes,recognizing
R5510	T8645	T8642	acomp	intense,was
R5511	T8646	T8642	prep	in,was
R5512	T8647	T8648	amod	homozygous,cells
R5513	T8648	T8646	pobj	cells,in
R5514	T8649	T8648	nmod	CbCln3Δex7,cells
R5515	T8650	T8649	punct	/,CbCln3Δex7
R5516	T8651	T8649	nummod	8,CbCln3Δex7
R5517	T8652	T8642	punct	.,was
R5518	T8654	T8655	amod	Wild,type
R5519	T8655	T8657	nsubjpass	type,captured
R552	T999	T974	punct	.,harbor
R5520	T8656	T8655	punct	-,type
R5521	T8658	T8655	cc	and,type
R5522	T8659	T8660	amod	homozygous,CbCln3Δex7
R5523	T8660	T8661	nmod	CbCln3Δex7,images
R5524	T8661	T8655	conj	images,type
R5525	T8662	T8660	punct	/,CbCln3Δex7
R5526	T8663	T8660	nummod	8,CbCln3Δex7
R5527	T8664	T8661	amod	confocal,images
R5528	T8665	T8657	auxpass	were,captured
R5529	T8666	T8657	prep	with,captured
R553	T1001	T1002	amod	Homozygous,mice
R5530	T8667	T8668	amod	identical,settings
R5531	T8668	T8666	pobj	settings,with
R5532	T8669	T8668	compound	exposure,settings
R5533	T8670	T8657	punct	.,captured
R5534	T8672	T8673	nummod	60,magnification
R5535	T8674	T8672	punct	×,60
R5536	T8675	T8673	punct	.,magnification
R5537	T8805	T8806	compound	Subunit,c
R5538	T8806	T8807	compound	c,accumulation
R5539	T8808	T8807	prep	in,accumulation
R554	T1002	T1009	nsubj	mice,exhibit
R5540	T8809	T8810	amod	homozygous,cells
R5541	T8810	T8808	pobj	cells,in
R5542	T8811	T8810	nmod	CbCln3Δex7,cells
R5543	T8812	T8811	punct	/,CbCln3Δex7
R5544	T8813	T8811	nummod	8,CbCln3Δex7
R5545	T8814	T8815	amod	cerebellar,precursor
R5546	T8815	T8810	compound	precursor,cells
R5547	T8817	T8818	meta	a,shown
R5548	T8819	T8817	punct	.,a
R5549	T8820	T8821	compound	Subunit,c
R555	T1003	T1002	nmod	Cln3Δex7,mice
R5550	T8821	T8818	nsubjpass	c,shown
R5551	T8822	T8821	appos	immunostaining,c
R5552	T8823	T8822	cc	and,immunostaining
R5553	T8824	T8822	conj	autofluorescence,immunostaining
R5554	T8825	T8821	prep	of,c
R5555	T8826	T8827	nummod	7,day
R5556	T8827	T8829	compound	day,confluency
R5557	T8828	T8827	punct	-,day
R5558	T8829	T8830	npadvmod	confluency,aged
R5559	T8830	T8832	amod	aged,cells
R556	T1004	T1003	punct	/,Cln3Δex7
R5560	T8831	T8830	punct	-,aged
R5561	T8832	T8825	pobj	cells,of
R5562	T8833	T8834	amod	wild,type
R5563	T8834	T8832	nmod	type,cells
R5564	T8835	T8834	punct	-,type
R5565	T8836	T8834	cc	and,type
R5566	T8837	T8834	conj	homozygous,type
R5567	T8838	T8832	nmod	CbCln3Δex7,cells
R5568	T8839	T8838	punct	/,CbCln3Δex7
R5569	T8840	T8838	nummod	8,CbCln3Δex7
R557	T1005	T1003	nummod	8,Cln3Δex7
R5570	T8841	T8818	auxpass	is,shown
R5571	T8842	T8818	punct	.,shown
R5572	T8844	T8845	amod	Wild,type
R5573	T8845	T8847	compound	type,cultures
R5574	T8846	T8845	punct	-,type
R5575	T8847	T8848	nsubj	cultures,exhibited
R5576	T8849	T8847	punct	(,cultures
R5577	T8850	T8847	appos	CbCln3,cultures
R5578	T8851	T8850	punct	+,CbCln3
R5579	T8852	T8850	punct	/,CbCln3
R558	T1006	T1002	amod	knock,mice
R5580	T8853	T8850	punct	+,CbCln3
R5581	T8854	T8848	punct	),exhibited
R5582	T8855	T8856	amod	limited,c
R5583	T8856	T8848	dobj	c,exhibited
R5584	T8857	T8856	compound	subunit,c
R5585	T8858	T8856	appos	immunostaining,c
R5586	T8859	T8858	cc	and,immunostaining
R5587	T8860	T8858	conj	autofluorescence,immunostaining
R5588	T8861	T8848	punct	.,exhibited
R5589	T8863	T8864	advmod	However,contained
R559	T1007	T1006	punct	-,knock
R5590	T8865	T8864	punct	", ",contained
R5591	T8866	T8867	nmod	CbCln3Δex7,Δex7
R5592	T8867	T8871	nmod	Δex7,cells
R5593	T8868	T8867	punct	/,Δex7
R5594	T8869	T8867	nummod	8,Δex7
R5595	T8870	T8867	punct	/,Δex7
R5596	T8871	T8864	nsubj	cells,contained
R5597	T8872	T8867	punct	/,Δex7
R5598	T8873	T8867	nummod	8,Δex7
R5599	T8874	T8875	amod	numerous,puncta
R56	T233	T229	npadvmod	c,synthase
R560	T1008	T1006	prt	in,knock
R5600	T8875	T8864	dobj	puncta,contained
R5601	T8876	T8877	compound	subunit,c
R5602	T8877	T8875	compound	c,puncta
R5603	T8878	T8864	punct	.,contained
R5604	T8880	T8881	nsubj	Autofluorescence,was
R5605	T8882	T8883	punct	(,AF
R5606	T8883	T8880	parataxis	AF,Autofluorescence
R5607	T8884	T8885	nummod	7,days
R5608	T8885	T8883	compound	days,AF
R5609	T8886	T8883	punct	),AF
R561	T1010	T1011	det	a,disease
R5610	T8887	T8881	advmod	also,was
R5611	T8888	T8889	advmod	significantly,elevated
R5612	T8889	T8881	acomp	elevated,was
R5613	T8890	T8891	punct	(,panels
R5614	T8891	T8889	parataxis	panels,elevated
R5615	T8892	T8891	amod	right,panels
R5616	T8893	T8891	punct	),panels
R5617	T8894	T8881	punct	", ",was
R5618	T8895	T8896	mark	although,observed
R5619	T8896	T8881	advcl	observed,was
R562	T1011	T1009	dobj	disease,exhibit
R5620	T8897	T8898	amod	limited,overlap
R5621	T8898	T8896	nsubjpass	overlap,observed
R5622	T8899	T8898	prep	with,overlap
R5623	T8900	T8901	compound	subunit,c
R5624	T8901	T8902	compound	c,puncta
R5625	T8902	T8899	pobj	puncta,with
R5626	T8903	T8896	auxpass	was,observed
R5627	T8904	T8905	punct	(,arrows
R5628	T8905	T8896	parataxis	arrows,observed
R5629	T8906	T8905	punct	),arrows
R563	T1012	T1011	amod	progressive,disease
R5630	T8907	T8881	punct	.,was
R5631	T8909	T8910	nummod	40,magnification
R5632	T8911	T8909	punct	×,40
R5633	T8912	T8910	punct	.,magnification
R5634	T8914	T8915	meta	b,shown
R5635	T8916	T8914	punct	.,b
R5636	T8917	T8918	compound	Immunoblot,analysis
R5637	T8918	T8915	nsubjpass	analysis,shown
R5638	T8919	T8918	prep	of,analysis
R5639	T8920	T8921	compound	subunit,c
R564	T1013	T1014	npadvmod	JNCL,like
R5640	T8921	T8922	compound	c,protein
R5641	T8922	T8919	pobj	protein,of
R5642	T8923	T8918	prep	at,analysis
R5643	T8924	T8925	nmod	sub-confluency,incubation
R5644	T8925	T8923	pobj	incubation,at
R5645	T8926	T8924	cc	or,sub-confluency
R5646	T8927	T8928	nummod	7,day
R5647	T8928	T8930	compound	day,confluency
R5648	T8929	T8928	punct	-,day
R5649	T8930	T8924	conj	confluency,sub-confluency
R565	T1014	T1011	amod	like,disease
R5650	T8931	T8915	auxpass	is,shown
R5651	T8932	T8915	punct	.,shown
R5652	T8934	T8935	amod	Total,extracts
R5653	T8935	T8937	nsubj	extracts,contained
R5654	T8936	T8935	compound	protein,extracts
R5655	T8938	T8935	prep	from,extracts
R5656	T8939	T8940	nmod	sub-confluency,cultures
R5657	T8940	T8938	pobj	cultures,from
R5658	T8941	T8942	amod	wild,type
R5659	T8942	T8940	nmod	type,cultures
R566	T1015	T1014	punct	-,like
R5660	T8943	T8942	punct	-,type
R5661	T8944	T8942	punct	(,type
R5662	T8945	T8946	punct	+,+
R5663	T8946	T8942	appos	+,type
R5664	T8947	T8946	punct	/,+
R5665	T8948	T8942	punct	),type
R5666	T8949	T8942	cc	and,type
R5667	T8950	T8951	amod	homozygous,mutant
R5668	T8951	T8942	conj	mutant,type
R5669	T8952	T8951	punct	(,mutant
R567	T1016	T1011	punct	", ",disease
R5670	T8953	T8951	appos	Δex7,mutant
R5671	T8954	T8953	punct	/,Δex7
R5672	T8955	T8953	nummod	8,Δex7
R5673	T8956	T8953	punct	/,Δex7
R5674	T8957	T8953	appos	Δex7,Δex7
R5675	T8958	T8957	punct	/,Δex7
R5676	T8959	T8957	nummod	8,Δex7
R5677	T8960	T8940	punct	),cultures
R5678	T8961	T8962	advmod	approximately,equal
R5679	T8962	T8963	amod	equal,levels
R568	T1017	T1011	prep	with,disease
R5680	T8963	T8937	dobj	levels,contained
R5681	T8964	T8963	prep	of,levels
R5682	T8965	T8966	compound	subunit,c
R5683	T8966	T8967	compound	c,protein
R5684	T8967	T8964	pobj	protein,of
R5685	T8968	T8967	punct	(,protein
R5686	T8969	T8970	nmod	α,c
R5687	T8970	T8967	appos	c,protein
R5688	T8971	T8969	punct	-,α
R5689	T8972	T8969	amod	sub,α
R569	T1018	T1019	amod	perinatal,onset
R5690	T8973	T8937	punct	),contained
R5691	T8974	T8937	punct	.,contained
R5692	T8976	T8977	nummod	7,day
R5693	T8977	T8979	compound	day,confluency
R5694	T8978	T8977	punct	-,day
R5695	T8979	T8980	compound	confluency,extract
R5696	T8980	T8981	nsubj	extract,had
R5697	T8982	T8980	prep	from,extract
R5698	T8983	T8984	amod	homozygous,cells
R5699	T8984	T8982	pobj	cells,from
R57	T234	T226	cc	and,deposition
R570	T1019	T1017	pobj	onset,with
R5700	T8985	T8984	nmod	CbCln3Δex7,cells
R5701	T8986	T8985	punct	/,CbCln3Δex7
R5702	T8987	T8985	nummod	8,CbCln3Δex7
R5703	T8988	T8984	punct	(,cells
R5704	T8989	T8984	appos	Δex7,cells
R5705	T8990	T8989	punct	/,Δex7
R5706	T8991	T8989	nummod	8,Δex7
R5707	T8992	T8989	punct	/,Δex7
R5708	T8993	T8989	appos	Δex7,Δex7
R5709	T8994	T8993	punct	/,Δex7
R571	T1020	T1019	prep	of,onset
R5710	T8995	T8993	nummod	8,Δex7
R5711	T8996	T8981	punct	),had
R5712	T8997	T8998	amod	elevated,levels
R5713	T8998	T8981	dobj	levels,had
R5714	T8999	T8998	prep	of,levels
R5715	T9000	T9001	compound	subunit,c
R5716	T9001	T9002	compound	c,protein
R5717	T9002	T8999	pobj	protein,of
R5718	T9003	T8998	punct	(,levels
R5719	T9004	T9005	punct	~,1.5
R572	T1021	T1022	compound	subunit,c
R5720	T9005	T8998	npadvmod	1.5,levels
R5721	T9006	T9005	punct	X,1.5
R5722	T9007	T8998	punct	),levels
R5723	T9008	T8998	punct	", ",levels
R5724	T9009	T8998	amod	relative,levels
R5725	T9010	T9009	prep	to,relative
R5726	T9011	T9012	amod	wild,type
R5727	T9012	T9014	compound	type,extract
R5728	T9013	T9012	punct	-,type
R5729	T9014	T9010	pobj	extract,to
R573	T1022	T1023	compound	c,deposition
R5730	T9015	T9014	punct	(,extract
R5731	T9016	T9017	punct	+,+
R5732	T9017	T9014	appos	+,extract
R5733	T9018	T9017	punct	/,+
R5734	T9019	T8981	punct	),had
R5735	T9020	T8981	punct	.,had
R5736	T9022	T9023	compound	Protein,levels
R5737	T9023	T9024	nsubjpass	levels,normalized
R5738	T9025	T9024	auxpass	were,normalized
R5739	T9026	T9024	prep	to,normalized
R574	T1023	T1020	pobj	deposition,of
R5740	T9027	T9028	compound	cytochrome,c
R5741	T9028	T9029	compound	c,oxidase
R5742	T9029	T9030	compound	oxidase,subunit
R5743	T9030	T9026	pobj	subunit,to
R5744	T9031	T9030	nummod	IV,subunit
R5745	T9032	T9030	punct	(,subunit
R5746	T9033	T9034	compound	α,cox4
R5747	T9034	T9030	appos	cox4,subunit
R5748	T9035	T9034	punct	-,cox4
R5749	T9036	T9024	punct	),normalized
R575	T1024	T1023	prep	in,deposition
R5750	T9037	T9024	punct	.,normalized
R5751	T9039	T9040	meta	c,shown
R5752	T9041	T9039	punct	.,c
R5753	T9042	T9043	compound	TEM,analysis
R5754	T9043	T9040	nsubjpass	analysis,shown
R5755	T9044	T9043	prep	of,analysis
R5756	T9045	T9044	pobj	inclusions,of
R5757	T9046	T9043	prep	in,analysis
R5758	T9047	T9048	nummod	7,day
R5759	T9048	T9050	compound	day,confluency
R576	T1025	T1026	amod	many,types
R5760	T9049	T9048	punct	-,day
R5761	T9050	T9051	npadvmod	confluency,aged
R5762	T9051	T9053	amod	aged,cells
R5763	T9052	T9051	punct	-,aged
R5764	T9053	T9046	pobj	cells,in
R5765	T9054	T9053	amod	homozygous,cells
R5766	T9055	T9053	nmod	CbCln3Δex7,cells
R5767	T9056	T9055	punct	/,CbCln3Δex7
R5768	T9057	T9055	nummod	8,CbCln3Δex7
R5769	T9058	T9040	auxpass	is,shown
R577	T1026	T1024	pobj	types,in
R5770	T9059	T9040	punct	.,shown
R5771	T9061	T9062	det	A,autophagosome
R5772	T9062	T9064	nsubjpass	autophagosome,filled
R5773	T9063	T9062	amod	large,autophagosome
R5774	T9065	T9062	acl	contained,autophagosome
R5775	T9066	T9065	agent	by,contained
R5776	T9067	T9068	amod	double,membrane
R5777	T9068	T9066	pobj	membrane,by
R5778	T9069	T9070	punct	(,arrows
R5779	T9070	T9065	parataxis	arrows,contained
R578	T1027	T1026	compound	cell,types
R5780	T9071	T9070	punct	),arrows
R5781	T9072	T9064	auxpass	is,filled
R5782	T9073	T9064	prep	with,filled
R5783	T9074	T9075	amod	degenerating,mitochondria
R5784	T9075	T9073	pobj	mitochondria,with
R5785	T9076	T9075	punct	(,mitochondria
R5786	T9077	T9075	appos	Md,mitochondria
R5787	T9078	T9075	punct	),mitochondria
R5788	T9079	T9075	punct	", ",mitochondria
R5789	T9080	T9081	nmod	electron,cores
R579	T1028	T1019	cc	and,onset
R5790	T9081	T9075	conj	cores,mitochondria
R5791	T9082	T9081	amod	dense,cores
R5792	T9083	T9084	punct	(,left
R5793	T9084	T9081	parataxis	left,cores
R5794	T9085	T9084	cc	and,left
R5795	T9086	T9084	conj	right,left
R5796	T9087	T9084	prep	of,left
R5797	T9088	T9087	punct	*,of
R5798	T9089	T9084	punct	),left
R5799	T9090	T9081	cc	and,cores
R58	T235	T236	det	a,loss
R580	T1029	T1030	amod	later,onset
R5800	T9091	T9092	amod	other,structures
R5801	T9092	T9081	conj	structures,cores
R5802	T9093	T9092	amod	smaller,structures
R5803	T9094	T9092	amod	vesicular,structures
R5804	T9095	T9064	punct	.,filled
R5805	T9097	T9098	det	A,inclusion
R5806	T9098	T9103	nsubj	inclusion,is
R5807	T9099	T9098	amod	large,inclusion
R5808	T9100	T9101	npadvmod	electron,dense
R5809	T9101	T9098	amod	dense,inclusion
R581	T1030	T1019	conj	onset,onset
R5810	T9102	T9101	punct	-,dense
R5811	T9104	T9098	punct	", ",inclusion
R5812	T9105	T9098	prep	with,inclusion
R5813	T9106	T9107	det	a,appearance
R5814	T9107	T9105	pobj	appearance,with
R5815	T9108	T9107	nmod	lipofuscin,appearance
R5816	T9109	T9108	punct	(,lipofuscin
R5817	T9110	T9108	appos	Ln,lipofuscin
R5818	T9111	T9107	punct	),appearance
R5819	T9112	T9103	punct	", ",is
R582	T1031	T1030	prep	of,onset
R5820	T9113	T9103	advmod	also,is
R5821	T9114	T9103	acomp	present,is
R5822	T9115	T9103	punct	.,is
R5823	T9118	T9117	punct	", ",M
R5824	T9119	T9117	appos	mitochondria,M
R5825	T9120	T9117	punct	.,M
R5826	T9122	T9123	nummod	"10,000",magnification
R5827	T9124	T9122	punct	×,"10,000"
R5828	T9125	T9123	punct	.,magnification
R5829	T9254	T9255	compound	Cathepsin,D
R583	T1032	T1033	amod	neuronal,dysfunction
R5830	T9256	T9255	appos	localization,D
R5831	T9257	T9256	cc	and,localization
R5832	T9258	T9256	conj	processing,localization
R5833	T9259	T9255	prep	in,D
R5834	T9260	T9261	amod	wild,type
R5835	T9261	T9263	nmod	type,cells
R5836	T9262	T9261	punct	-,type
R5837	T9263	T9259	pobj	cells,in
R5838	T9264	T9261	cc	and,type
R5839	T9265	T9261	conj	homozygous,type
R584	T1033	T1031	pobj	dysfunction,of
R5840	T9266	T9263	nmod	CbCln3Δex7,cells
R5841	T9267	T9266	punct	/,CbCln3Δex7
R5842	T9268	T9266	nummod	8,CbCln3Δex7
R5843	T9270	T9271	meta	a,shown
R5844	T9272	T9270	punct	.,a
R5845	T9273	T9271	nsubjpass	Immunostaining,shown
R5846	T9274	T9273	prep	of,Immunostaining
R5847	T9275	T9276	amod	wild,type
R5848	T9276	T9278	nmod	type,cells
R5849	T9277	T9276	punct	-,type
R585	T1034	T1033	cc	and,dysfunction
R5850	T9278	T9274	pobj	cells,of
R5851	T9279	T9276	cc	and,type
R5852	T9280	T9276	conj	homozygous,type
R5853	T9281	T9278	nmod	CbCln3Δex7,cells
R5854	T9282	T9281	punct	/,CbCln3Δex7
R5855	T9283	T9281	nummod	8,CbCln3Δex7
R5856	T9284	T9278	compound	precursor,cells
R5857	T9285	T9273	prep	with,Immunostaining
R5858	T9286	T9287	amod	anti-cathepsin,D
R5859	T9287	T9288	amod	D,antibody
R586	T1035	T1036	amod	behavioral,deficits
R5860	T9288	T9285	pobj	antibody,with
R5861	T9289	T9273	punct	", ",Immunostaining
R5862	T9290	T9273	acl	recognizing,Immunostaining
R5863	T9291	T9292	amod	unprocessed,forms
R5864	T9292	T9290	dobj	forms,recognizing
R5865	T9293	T9291	cc	and,unprocessed
R5866	T9294	T9291	conj	processed,unprocessed
R5867	T9295	T9292	prep	of,forms
R5868	T9296	T9297	compound	cathepsin,D
R5869	T9297	T9298	compound	D,protein
R587	T1036	T1033	conj	deficits,dysfunction
R5870	T9298	T9295	pobj	protein,of
R5871	T9299	T9271	auxpass	is,shown
R5872	T9300	T9271	punct	.,shown
R5873	T9302	T9303	nmod	CbCln3,cells
R5874	T9303	T9307	nsubj	cells,exhibited
R5875	T9304	T9302	punct	+,CbCln3
R5876	T9305	T9302	punct	/,CbCln3
R5877	T9306	T9302	punct	+,CbCln3
R5878	T9308	T9309	punct	(,panel
R5879	T9309	T9303	parataxis	panel,cells
R588	T1037	T1038	punct	[,12
R5880	T9310	T9309	amod	left,panel
R5881	T9311	T9309	punct	),panel
R5882	T9312	T9313	det	a,signal
R5883	T9313	T9307	dobj	signal,exhibited
R5884	T9314	T9313	amod	perinuclear,signal
R5885	T9315	T9314	cc	and,perinuclear
R5886	T9316	T9314	conj	cytoplasmic,perinuclear
R5887	T9317	T9313	amod	punctate,signal
R5888	T9318	T9307	punct	.,exhibited
R5889	T9320	T9321	compound	Cathepsin,D
R589	T1038	T1009	parataxis	12,exhibit
R5890	T9321	T9322	compound	D,signal
R5891	T9322	T9323	nsubj	signal,was
R5892	T9324	T9322	prep	in,signal
R5893	T9325	T9326	amod	homozygous,cells
R5894	T9326	T9324	pobj	cells,in
R5895	T9327	T9326	nmod	CbCln3Δex7,cells
R5896	T9328	T9327	punct	/,CbCln3Δex7
R5897	T9329	T9327	nummod	8,CbCln3Δex7
R5898	T9330	T9331	punct	(,panel
R5899	T9331	T9322	parataxis	panel,signal
R59	T236	T226	conj	loss,deposition
R590	T1039	T1038	punct	],12
R5900	T9332	T9331	amod	right,panel
R5901	T9333	T9331	punct	),panel
R5902	T9334	T9335	advmod	more,often
R5903	T9335	T9323	advmod	often,was
R5904	T9336	T9323	acomp	perinuclear,was
R5905	T9337	T9323	punct	", ",was
R5906	T9338	T9323	prep	with,was
R5907	T9339	T9340	advmod	less,signal
R5908	T9340	T9338	pobj	signal,with
R5909	T9341	T9342	amod	cytoplasmic,punctate
R591	T1040	T1009	punct	.,exhibit
R5910	T9342	T9340	compound	punctate,signal
R5911	T9343	T9340	punct	", ",signal
R5912	T9344	T9340	prep	compared,signal
R5913	T9345	T9344	prep	to,compared
R5914	T9346	T9347	amod	wild,type
R5915	T9347	T9349	nmod	type,cells
R5916	T9348	T9347	punct	-,type
R5917	T9349	T9345	pobj	cells,to
R5918	T9350	T9349	nmod	CbCln3,cells
R5919	T9351	T9350	punct	+,CbCln3
R592	T1042	T1043	det	These,findings
R5920	T9352	T9350	punct	/,CbCln3
R5921	T9353	T9350	punct	+,CbCln3
R5922	T9354	T9323	punct	.,was
R5923	T9356	T9357	nummod	40,magnification
R5924	T9358	T9356	punct	×,40
R5925	T9359	T9357	punct	.,magnification
R5926	T9361	T9362	meta	b,shown
R5927	T9363	T9361	punct	.,b
R5928	T9364	T9365	compound	α,D
R5929	T9365	T9368	npadvmod	D,probed
R593	T1043	T1044	nsubj	findings,suggest
R5930	T9366	T9365	punct	-,D
R5931	T9367	T9365	compound	Cathepsin,D
R5932	T9368	T9370	amod	probed,immunoblots
R5933	T9369	T9368	punct	-,probed
R5934	T9370	T9362	nsubjpass	immunoblots,shown
R5935	T9371	T9370	prep	of,immunoblots
R5936	T9372	T9373	amod	total,tissue
R5937	T9373	T9371	pobj	tissue,of
R5938	T9374	T9375	amod	wild,type
R5939	T9375	T9373	nmod	type,tissue
R594	T1045	T1046	mark	that,leads
R5940	T9376	T9375	punct	-,type
R5941	T9377	T9375	cc	versus,type
R5942	T9378	T9379	amod	homozygous,Cln3Δex7
R5943	T9379	T9380	npadvmod	Cln3Δex7,knock
R5944	T9380	T9375	conj	knock,type
R5945	T9381	T9379	punct	/,Cln3Δex7
R5946	T9382	T9379	nummod	8,Cln3Δex7
R5947	T9383	T9380	punct	-,knock
R5948	T9384	T9380	prt	in,knock
R5949	T9385	T9373	cc	or,tissue
R595	T1046	T1044	ccomp	leads,suggest
R5950	T9386	T9387	nmod	CbCln3Δex7,extracts
R5951	T9387	T9373	conj	extracts,tissue
R5952	T9388	T9386	punct	/,CbCln3Δex7
R5953	T9389	T9386	nummod	8,CbCln3Δex7
R5954	T9390	T9387	amod	cellular,extracts
R5955	T9391	T9362	auxpass	are,shown
R5956	T9392	T9362	punct	.,shown
R5957	T9394	T9395	det	The,band
R5958	T9395	T9401	nsubj	band,was
R5959	T9396	T9397	punct	~,45
R596	T1047	T1048	det	the,defect
R5960	T9397	T9398	nummod	45,kDa
R5961	T9398	T9395	compound	kDa,band
R5962	T9399	T9400	compound	cathepsin,D
R5963	T9400	T9395	compound	D,band
R5964	T9402	T9395	punct	", ",band
R5965	T9403	T9395	acl	representing,band
R5966	T9404	T9403	dobj	precursor,representing
R5967	T9405	T9401	punct	", ",was
R5968	T9406	T9407	det	the,band
R5969	T9407	T9401	attr	band,was
R597	T1048	T1046	nsubj	defect,leads
R5970	T9408	T9407	amod	predominant,band
R5971	T9409	T9407	prep	in,band
R5972	T9410	T9411	amod	wild,type
R5973	T9411	T9409	pobj	type,in
R5974	T9412	T9411	punct	-,type
R5975	T9413	T9411	punct	(,type
R5976	T9414	T9411	appos	wt,type
R5977	T9415	T9411	punct	),type
R5978	T9416	T9411	appos	tissue,type
R5979	T9417	T9416	cc	and,tissue
R598	T1049	T1048	amod	major,defect
R5980	T9418	T9419	amod	cellular,extracts
R5981	T9419	T9416	conj	extracts,tissue
R5982	T9420	T9407	punct	", ",band
R5983	T9421	T9407	prep	with,band
R5984	T9422	T9423	amod	lower,levels
R5985	T9423	T9421	pobj	levels,with
R5986	T9424	T9423	prep	of,levels
R5987	T9425	T9426	amod	mature,enzyme
R5988	T9426	T9424	pobj	enzyme,of
R5989	T9427	T9428	punct	(,kDa
R599	T1050	T1048	compound	JNCL,defect
R5990	T9428	T9401	parataxis	kDa,was
R5991	T9429	T9430	amod	single,chain
R5992	T9430	T9428	dep	chain,kDa
R5993	T9431	T9428	punct	", ",kDa
R5994	T9432	T9433	punct	~,43
R5995	T9433	T9428	nummod	43,kDa
R5996	T9434	T9428	punct	", ",kDa
R5997	T9435	T9428	cc	and,kDa
R5998	T9436	T9437	amod	heavy,chain
R5999	T9437	T9438	dep	chain,kDa
R6	T175	T176	amod	mitochondrial,abnormalities
R60	T237	T236	amod	specific,loss
R600	T1051	T1046	prep	to,leads
R6000	T9438	T9428	conj	kDa,kDa
R6001	T9439	T9438	punct	", ",kDa
R6002	T9440	T9441	punct	~,31
R6003	T9441	T9438	nummod	31,kDa
R6004	T9442	T9438	punct	),kDa
R6005	T9443	T9401	punct	.,was
R6006	T9445	T9446	advmod	Conversely,exhibited
R6007	T9447	T9446	punct	", ",exhibited
R6008	T9448	T9449	amod	homozygous,extracts
R6009	T9449	T9446	nsubj	extracts,exhibited
R601	T1052	T1053	amod	abnormal,turnover
R6010	T9450	T9449	nmod	Cln3Δex7,extracts
R6011	T9451	T9450	punct	/,Cln3Δex7
R6012	T9452	T9450	nummod	8,Cln3Δex7
R6013	T9453	T9450	cc	and,Cln3Δex7
R6014	T9454	T9450	conj	CbCln3Δex7,Cln3Δex7
R6015	T9455	T9454	punct	/,CbCln3Δex7
R6016	T9456	T9454	nummod	8,CbCln3Δex7
R6017	T9457	T9449	nmod	mutant,extracts
R6018	T9458	T9457	punct	(,mutant
R6019	T9459	T9457	appos	m,mutant
R602	T1053	T1051	pobj	turnover,to
R6020	T9460	T9449	punct	),extracts
R6021	T9461	T9462	amod	reduced,levels
R6022	T9462	T9446	dobj	levels,exhibited
R6023	T9463	T9462	prep	of,levels
R6024	T9464	T9463	pobj	precursor,of
R6025	T9465	T9464	cc	and,precursor
R6026	T9466	T9467	amod	heavy,chain
R6027	T9467	T9464	conj	chain,precursor
R6028	T9468	T9464	prep	of,precursor
R6029	T9469	T9470	det	the,form
R603	T1054	T1053	prep	of,turnover
R6030	T9470	T9468	pobj	form,of
R6031	T9471	T9472	amod	double,chain
R6032	T9472	T9470	compound	chain,form
R6033	T9473	T9472	punct	-,chain
R6034	T9474	T9470	prep	of,form
R6035	T9475	T9476	det	the,enzyme
R6036	T9476	T9474	pobj	enzyme,of
R6037	T9477	T9446	punct	", ",exhibited
R6038	T9478	T9446	prep	with,exhibited
R6039	T9479	T9480	amod	elevated,levels
R604	T1055	T1056	amod	mitochondrial,c
R6040	T9480	T9478	pobj	levels,with
R6041	T9481	T9480	prep	of,levels
R6042	T9482	T9483	amod	single,chain
R6043	T9483	T9485	nmod	chain,enzyme
R6044	T9484	T9483	punct	-,chain
R6045	T9485	T9481	pobj	enzyme,of
R6046	T9486	T9485	amod	mature,enzyme
R6047	T9487	T9446	punct	.,exhibited
R605	T1056	T1054	pobj	c,of
R6050	T9581	T9582	nmod	Lysotracker,labeling
R6051	T9582	T9586	dep	labeling,shown
R6052	T9583	T9581	cc	and,Lysotracker
R6053	T9584	T9581	conj	Lamp,Lysotracker
R6054	T9585	T9584	nummod	2,Lamp
R6055	T9587	T9582	prep	of,labeling
R6056	T9588	T9589	amod	wild,type
R6057	T9589	T9591	nmod	type,lysosomes
R6058	T9590	T9589	punct	-,type
R6059	T9591	T9587	pobj	lysosomes,of
R606	T1057	T1056	compound	subunit,c
R6060	T9592	T9589	cc	and,type
R6061	T9593	T9589	conj	homozygous,type
R6062	T9594	T9591	nmod	CbCln3Δex7,lysosomes
R6063	T9595	T9594	punct	/,CbCln3Δex7
R6064	T9596	T9594	nummod	8,CbCln3Δex7
R6065	T9597	T9598	amod	Lysosomal,labeling
R6066	T9598	T9586	nsubjpass	labeling,shown
R6067	T9599	T9598	prep	of,labeling
R6068	T9600	T9601	amod	wild,type
R6069	T9601	T9603	nmod	type,cells
R607	T1058	T1046	punct	", ",leads
R6070	T9602	T9601	punct	-,type
R6071	T9603	T9599	pobj	cells,of
R6072	T9604	T9601	cc	and,type
R6073	T9605	T9606	amod	homozygous,CbCln3Δex7
R6074	T9606	T9601	conj	CbCln3Δex7,type
R6075	T9607	T9606	punct	/,CbCln3Δex7
R6076	T9608	T9606	nummod	8,CbCln3Δex7
R6077	T9609	T9603	compound	precursor,cells
R6078	T9610	T9598	prep	with,labeling
R6079	T9611	T9612	nmod	lysotracker,antibody
R608	T1059	T1046	prep	in,leads
R6080	T9612	T9610	pobj	antibody,with
R6081	T9613	T9611	cc	and,lysotracker
R6082	T9614	T9611	conj	Lamp,lysotracker
R6083	T9615	T9614	nummod	2,Lamp
R6084	T9616	T9586	auxpass	is,shown
R6085	T9617	T9586	punct	.,shown
R6086	T9619	T9620	compound	Lysotracker,dye
R6087	T9620	T9621	nsubj	dye,labeled
R6088	T9622	T9623	punct	(,panels
R6089	T9623	T9620	parataxis	panels,dye
R609	T1060	T1061	det	a,manner
R6090	T9624	T9623	amod	top,panels
R6091	T9625	T9623	punct	),panels
R6092	T9626	T9627	amod	large,lysosomes
R6093	T9627	T9621	dobj	lysosomes,labeled
R6094	T9628	T9627	punct	", ",lysosomes
R6095	T9629	T9630	amod	perinuclear,clustered
R6096	T9630	T9627	amod	clustered,lysosomes
R6097	T9631	T9630	punct	-,clustered
R6098	T9632	T9627	cc	and,lysosomes
R6099	T9633	T9634	amod	scattered,lysosomes
R61	T238	T236	prep	of,loss
R610	T1061	T1059	pobj	manner,in
R6100	T9634	T9627	conj	lysosomes,lysosomes
R6101	T9635	T9621	prep	in,labeled
R6102	T9636	T9637	det	the,periphery
R6103	T9637	T9635	pobj	periphery,in
R6104	T9638	T9637	prep	of,periphery
R6105	T9639	T9640	amod	wild,type
R6106	T9640	T9642	compound	type,cells
R6107	T9641	T9640	punct	-,type
R6108	T9642	T9638	pobj	cells,of
R6109	T9643	T9642	punct	(,cells
R611	T1062	T1063	dep	that,compromises
R6110	T9644	T9642	appos	CbCln3,cells
R6111	T9645	T9644	punct	+,CbCln3
R6112	T9646	T9644	punct	/,CbCln3
R6113	T9647	T9644	punct	+,CbCln3
R6114	T9648	T9621	punct	),labeled
R6115	T9649	T9621	punct	.,labeled
R6116	T9651	T9652	compound	Lysotracker,stain
R6117	T9652	T9653	nsubj	stain,was
R6118	T9654	T9653	advmod	dramatically,was
R6119	T9655	T9653	acomp	reduced,was
R612	T1063	T1061	relcl	compromises,manner
R6120	T9656	T9653	prep	in,was
R6121	T9657	T9658	amod	homozygous,cells
R6122	T9658	T9656	pobj	cells,in
R6123	T9659	T9658	compound	mutant,cells
R6124	T9660	T9658	punct	(,cells
R6125	T9661	T9658	appos	CbCln3Δex7,cells
R6126	T9662	T9661	punct	/,CbCln3Δex7
R6127	T9663	T9661	nummod	8,CbCln3Δex7
R6128	T9664	T9661	punct	/,CbCln3Δex7
R6129	T9665	T9661	appos	Δex7,CbCln3Δex7
R613	T1064	T1063	advmod	selectively,compromises
R6130	T9666	T9665	punct	/,Δex7
R6131	T9667	T9665	nummod	8,Δex7
R6132	T9668	T9653	punct	),was
R6133	T9669	T9653	punct	", ",was
R6134	T9670	T9653	prep	with,was
R6135	T9671	T9672	amod	smaller,vesicles
R6136	T9672	T9670	pobj	vesicles,with
R6137	T9673	T9672	amod	labeled,vesicles
R6138	T9674	T9672	cc	and,vesicles
R6139	T9675	T9676	advmod	less,apparent
R614	T1065	T1066	compound	CNS,neurons
R6140	T9676	T9677	amod	apparent,clustering
R6141	T9677	T9672	conj	clustering,vesicles
R6142	T9678	T9677	amod	perinuclear,clustering
R6143	T9679	T9653	punct	.,was
R6144	T9681	T9682	nmod	Lamp,immunostaining
R6145	T9682	T9688	nsubj	immunostaining,showed
R6146	T9683	T9681	nummod	2,Lamp
R6147	T9684	T9685	punct	(,panels
R6148	T9685	T9681	parataxis	panels,Lamp
R6149	T9686	T9685	amod	bottom,panels
R615	T1066	T1063	dobj	neurons,compromises
R6150	T9687	T9685	punct	),panels
R6151	T9689	T9688	advmod	also,showed
R6152	T9690	T9691	amod	reduced,intensity
R6153	T9691	T9688	dobj	intensity,showed
R6154	T9692	T9691	compound	signal,intensity
R6155	T9693	T9688	prep	with,showed
R6156	T9694	T9695	advmod	less,clustering
R6157	T9695	T9693	pobj	clustering,with
R6158	T9696	T9695	amod	perinuclear,clustering
R6159	T9697	T9688	prep	in,showed
R616	T1067	T1044	punct	.,suggest
R6160	T9698	T9699	amod	homozygous,cells
R6161	T9699	T9697	pobj	cells,in
R6162	T9700	T9699	nmod	CbCln3Δex7,cells
R6163	T9701	T9700	punct	/,CbCln3Δex7
R6164	T9702	T9700	nummod	8,CbCln3Δex7
R6165	T9703	T9688	punct	", ",showed
R6166	T9704	T9705	mark	although,was
R6167	T9705	T9688	advcl	was,showed
R6168	T9706	T9707	det	the,effect
R6169	T9707	T9705	nsubj	effect,was
R617	T1069	T1070	advmod	Currently,is
R6170	T9708	T9709	advmod	somewhat,less
R6171	T9709	T9710	advmod	less,dramatic
R6172	T9710	T9705	acomp	dramatic,was
R6173	T9711	T9710	prep	than,dramatic
R6174	T9712	T9711	pobj	that,than
R6175	T9713	T9712	acl	observed,that
R6176	T9714	T9713	prep	with,observed
R6177	T9715	T9716	compound	Lysotracker,dye
R6178	T9716	T9714	pobj	dye,with
R6179	T9717	T9688	punct	.,showed
R618	T1071	T1070	punct	", ",is
R6180	T9719	T9720	amod	Wild,type
R6181	T9720	T9722	nmod	type,images
R6182	T9721	T9720	punct	-,type
R6183	T9722	T9729	nsubjpass	images,captured
R6184	T9723	T9720	cc	and,type
R6185	T9724	T9720	conj	homozygous,type
R6186	T9725	T9722	nmod	CbCln3Δex7,images
R6187	T9726	T9725	punct	/,CbCln3Δex7
R6188	T9727	T9725	nummod	8,CbCln3Δex7
R6189	T9728	T9722	amod	confocal,images
R619	T1072	T1070	expl	there,is
R6190	T9730	T9729	auxpass	were,captured
R6191	T9731	T9729	prep	with,captured
R6192	T9732	T9733	amod	identical,settings
R6193	T9733	T9731	pobj	settings,with
R6194	T9734	T9733	compound	exposure,settings
R6195	T9735	T9729	punct	.,captured
R6196	T9737	T9738	nummod	60,magnification
R6197	T9739	T9737	punct	×,60
R6198	T9740	T9738	punct	.,magnification
R6199	T9816	T9817	dep	Endocytosis,shown
R62	T239	T240	compound	CNS,neurons
R620	T1073	T1074	det	no,system
R6200	T9818	T9816	prep	in,Endocytosis
R6201	T9819	T9820	amod	wild,type
R6202	T9820	T9822	nmod	type,cells
R6203	T9821	T9820	punct	-,type
R6204	T9822	T9818	pobj	cells,in
R6205	T9823	T9820	punct	", ",type
R6206	T9824	T9820	conj	heterozygous,type
R6207	T9825	T9824	cc	and,heterozygous
R6208	T9826	T9824	conj	homozygous,heterozygous
R6209	T9827	T9822	nmod	CbCln3Δex7,cells
R621	T1074	T1070	attr	system,is
R6210	T9828	T9827	punct	/,CbCln3Δex7
R6211	T9829	T9827	nummod	8,CbCln3Δex7
R6212	T9830	T9831	compound	Dextran,FITC
R6213	T9831	T9833	compound	FITC,uptake
R6214	T9832	T9831	punct	-,FITC
R6215	T9833	T9817	nsubjpass	uptake,shown
R6216	T9834	T9833	prep	in,uptake
R6217	T9835	T9836	amod	wild,type
R6218	T9836	T9838	nmod	type,cells
R6219	T9837	T9836	punct	-,type
R622	T1075	T1074	amod	suitable,system
R6220	T9838	T9834	pobj	cells,in
R6221	T9839	T9836	punct	", ",type
R6222	T9840	T9836	conj	heterozygous,type
R6223	T9841	T9840	cc	and,heterozygous
R6224	T9842	T9840	conj	homozygous,heterozygous
R6225	T9843	T9838	nmod	CbCln3Δex7,cells
R6226	T9844	T9843	punct	/,CbCln3Δex7
R6227	T9845	T9843	nummod	8,CbCln3Δex7
R6228	T9846	T9838	compound	precursor,cells
R6229	T9847	T9817	auxpass	is,shown
R623	T1076	T1077	amod	neuronal,cell
R6230	T9848	T9817	punct	.,shown
R6231	T9850	T9851	prep	In,observed
R6232	T9852	T9853	amod	wild,type
R6233	T9853	T9855	nmod	type,cells
R6234	T9854	T9853	punct	-,type
R6235	T9855	T9850	pobj	cells,In
R6236	T9856	T9853	punct	(,type
R6237	T9857	T9853	appos	CbCln3,type
R6238	T9858	T9857	punct	+,CbCln3
R6239	T9859	T9857	punct	/,CbCln3
R624	T1077	T1074	compound	cell,system
R6240	T9860	T9857	punct	+,CbCln3
R6241	T9861	T9862	punct	", ",panel
R6242	T9862	T9853	parataxis	panel,type
R6243	T9863	T9862	amod	left,panel
R6244	T9864	T9862	punct	),panel
R6245	T9865	T9853	cc	and,type
R6246	T9866	T9853	conj	heterozygous,type
R6247	T9867	T9868	punct	(,panel
R6248	T9868	T9866	parataxis	panel,heterozygous
R6249	T9869	T9870	nmod	CbCln3,Δex7
R625	T1078	T1079	aux	to,investigate
R6250	T9870	T9868	dep	Δex7,panel
R6251	T9871	T9870	punct	+,Δex7
R6252	T9872	T9870	punct	/,Δex7
R6253	T9873	T9870	punct	/,Δex7
R6254	T9874	T9870	nummod	8,Δex7
R6255	T9875	T9868	punct	", ",panel
R6256	T9876	T9868	amod	middle,panel
R6257	T9877	T9868	punct	),panel
R6258	T9878	T9851	punct	", ",observed
R6259	T9879	T9880	compound	dextran,FITC
R626	T1079	T1074	advcl	investigate,system
R6260	T9880	T9882	compound	FITC,label
R6261	T9881	T9880	punct	-,FITC
R6262	T9882	T9851	nsubjpass	label,observed
R6263	T9883	T9851	auxpass	was,observed
R6264	T9884	T9851	prep	in,observed
R6265	T9885	T9886	det	a,pattern
R6266	T9886	T9884	pobj	pattern,in
R6267	T9887	T9888	amod	perinuclear,clustered
R6268	T9888	T9886	amod	clustered,pattern
R6269	T9889	T9888	punct	-,clustered
R627	T1080	T1081	det	the,impact
R6270	T9890	T9886	amod	vesicular,pattern
R6271	T9891	T9851	prep	with,observed
R6272	T9892	T9893	amod	scattered,vesicles
R6273	T9893	T9891	pobj	vesicles,with
R6274	T9894	T9893	amod	labeled,vesicles
R6275	T9895	T9896	advmod	also,present
R6276	T9896	T9893	relcl	present,vesicles
R6277	T9897	T9896	prep	in,present
R6278	T9898	T9899	det	the,periphery
R6279	T9899	T9897	pobj	periphery,in
R628	T1081	T1079	dobj	impact,investigate
R6280	T9900	T9851	punct	.,observed
R6281	T9902	T9903	prep	In,reduced
R6282	T9904	T9902	pobj	contrast,In
R6283	T9905	T9903	punct	", ",reduced
R6284	T9906	T9907	compound	dextran,FITC
R6285	T9907	T9909	compound	FITC,label
R6286	T9908	T9907	punct	-,FITC
R6287	T9909	T9903	nsubjpass	label,reduced
R6288	T9910	T9909	prep	of,label
R6289	T9911	T9912	amod	homozygous,mutant
R629	T1082	T1081	prep	of,impact
R6290	T9912	T9913	nmod	mutant,cells
R6291	T9913	T9910	pobj	cells,of
R6292	T9914	T9912	punct	(,mutant
R6293	T9915	T9916	nmod	CbCln3Δex7,Δex7
R6294	T9916	T9912	appos	Δex7,mutant
R6295	T9917	T9916	punct	/,Δex7
R6296	T9918	T9916	nummod	8,Δex7
R6297	T9919	T9916	punct	/,Δex7
R6298	T9920	T9916	punct	/,Δex7
R6299	T9921	T9916	nummod	8,Δex7
R63	T240	T238	pobj	neurons,of
R630	T1083	T1084	det	the,mutation
R6300	T9922	T9923	punct	", ",panel
R6301	T9923	T9912	parataxis	panel,mutant
R6302	T9924	T9923	amod	right,panel
R6303	T9925	T9923	punct	),panel
R6304	T9926	T9903	auxpass	was,reduced
R6305	T9927	T9903	advmod	overall,reduced
R6306	T9928	T9903	cc	and,reduced
R6307	T9929	T9903	conj	exhibited,reduced
R6308	T9930	T9931	amod	smaller,vesicles
R6309	T9931	T9929	dobj	vesicles,exhibited
R631	T1084	T1082	pobj	mutation,of
R6310	T9932	T9931	amod	stained,vesicles
R6311	T9933	T9931	prep	with,vesicles
R6312	T9934	T9935	advmod	less,clustering
R6313	T9935	T9933	pobj	clustering,with
R6314	T9936	T9935	amod	perinuclear,clustering
R6315	T9937	T9903	punct	.,reduced
R6316	T9939	T9940	amod	Confocal,images
R6317	T9940	T9941	nsubjpass	images,captured
R6318	T9942	T9941	auxpass	were,captured
R6319	T9943	T9941	prep	with,captured
R632	T1085	T1084	amod	common,mutation
R6320	T9944	T9945	amod	identical,settings
R6321	T9945	T9943	pobj	settings,with
R6322	T9946	T9945	compound	exposure,settings
R6323	T9947	T9941	punct	.,captured
R6324	T9949	T9950	nummod	40,magnification
R6325	T9951	T9949	punct	×,40
R6326	T9952	T9950	punct	.,magnification
R6327	T10102	T10103	amod	Mitochondrial,morphology
R6328	T10104	T10103	cc	and,morphology
R6329	T10105	T10103	conj	function,morphology
R633	T1086	T1084	compound	JNCL,mutation
R6330	T10106	T10103	prep	in,morphology
R6331	T10107	T10108	amod	wild,type
R6332	T10108	T10110	nmod	type,cells
R6333	T10109	T10108	punct	-,type
R6334	T10110	T10106	pobj	cells,in
R6335	T10111	T10108	punct	", ",type
R6336	T10112	T10108	conj	heterozygous,type
R6337	T10113	T10112	cc	and,heterozygous
R6338	T10114	T10112	conj	homozygous,heterozygous
R6339	T10115	T10110	nmod	CbCln3Δex7,cells
R634	T1087	T1081	prep	on,impact
R6340	T10116	T10115	punct	/,CbCln3Δex7
R6341	T10117	T10115	nummod	8,CbCln3Δex7
R6342	T10119	T10120	meta	a,shown
R6343	T10121	T10119	punct	.,a
R6344	T10122	T10123	amod	Confocal,micrographs
R6345	T10123	T10120	nsubjpass	micrographs,shown
R6346	T10124	T10122	cc	and,Confocal
R6347	T10125	T10122	conj	TEM,Confocal
R6348	T10126	T10123	prep	of,micrographs
R6349	T10127	T10128	amod	wild,type
R635	T1088	T1089	amod	biological,processes
R6350	T10128	T10130	nmod	type,morphology
R6351	T10129	T10128	punct	-,type
R6352	T10130	T10126	pobj	morphology,of
R6353	T10131	T10128	cc	and,type
R6354	T10132	T10128	conj	homozygous,type
R6355	T10133	T10130	nmod	CbCln3Δex7,morphology
R6356	T10134	T10133	punct	/,CbCln3Δex7
R6357	T10135	T10133	nummod	8,CbCln3Δex7
R6358	T10136	T10130	amod	mitochondrial,morphology
R6359	T10137	T10120	auxpass	are,shown
R636	T1089	T1087	pobj	processes,on
R6360	T10138	T10120	punct	.,shown
R6361	T10140	T10141	nsubj	Immunostaining,highlighted
R6362	T10142	T10140	prep	with,Immunostaining
R6363	T10143	T10144	det	the,marker
R6364	T10144	T10142	pobj	marker,with
R6365	T10145	T10144	amod	inner,marker
R6366	T10146	T10144	amod	mitochondrial,marker
R6367	T10147	T10144	compound	membrane,marker
R6368	T10148	T10144	punct	", ",marker
R6369	T10149	T10144	appos	grp75,marker
R637	T1090	T1070	punct	.,is
R6370	T10150	T10151	punct	(,panels
R6371	T10151	T10140	parataxis	panels,Immunostaining
R6372	T10152	T10151	amod	top,panels
R6373	T10153	T10151	punct	),panels
R6374	T10154	T10155	amod	elongated,mitochondria
R6375	T10155	T10141	dobj	mitochondria,highlighted
R6376	T10156	T10141	prep	in,highlighted
R6377	T10157	T10158	amod	homozygous,cells
R6378	T10158	T10156	pobj	cells,in
R6379	T10159	T10158	compound	mutant,cells
R638	T1092	T1093	advmod	Therefore,established
R6380	T10160	T10158	punct	(,cells
R6381	T10161	T10162	nmod	CbCln3Δex7,Δex7
R6382	T10162	T10158	appos	Δex7,cells
R6383	T10163	T10162	punct	/,Δex7
R6384	T10164	T10162	nummod	8,Δex7
R6385	T10165	T10162	punct	/,Δex7
R6386	T10166	T10162	punct	/,Δex7
R6387	T10167	T10162	nummod	8,Δex7
R6388	T10168	T10141	punct	),highlighted
R6389	T10169	T10141	punct	", ",highlighted
R639	T1094	T1093	punct	", ",established
R6390	T10170	T10141	advcl	relative,highlighted
R6391	T10171	T10170	prep	to,relative
R6392	T10172	T10173	amod	wild,type
R6393	T10173	T10175	compound	type,mitochondria
R6394	T10174	T10173	punct	-,type
R6395	T10175	T10171	pobj	mitochondria,to
R6396	T10176	T10175	punct	(,mitochondria
R6397	T10177	T10175	appos	CbCln3,mitochondria
R6398	T10178	T10177	punct	+,CbCln3
R6399	T10179	T10177	punct	/,CbCln3
R64	T241	T221	punct	.,leads
R640	T1095	T1093	nsubj	we,established
R6400	T10180	T10177	punct	+,CbCln3
R6401	T10181	T10170	punct	),relative
R6402	T10182	T10183	punct	(,insets
R6403	T10183	T10170	parataxis	insets,relative
R6404	T10184	T10183	punct	", ",insets
R6405	T10185	T10186	nsubj	zoom,2.75
R6406	T10186	T10183	ccomp	2.75,insets
R6407	T10187	T10186	punct	=,2.75
R6408	T10188	T10186	punct	x,2.75
R6409	T10189	T10183	punct	),insets
R641	T1096	T1093	aux	have,established
R6410	T10190	T10141	punct	.,highlighted
R6411	T10192	T10193	amod	Mitochondrial,distribution
R6412	T10193	T10194	nsubjpass	distribution,altered
R6413	T10195	T10194	auxpass	was,altered
R6414	T10196	T10194	neg	not,altered
R6415	T10197	T10194	prep	from,altered
R6416	T10198	T10199	det	the,pattern
R6417	T10199	T10197	pobj	pattern,from
R6418	T10200	T10201	amod	wild,type
R6419	T10201	T10199	compound	type,pattern
R642	T1097	T1098	amod	cerebellar,lines
R6420	T10202	T10201	punct	-,type
R6421	T10203	T10194	punct	.,altered
R6422	T10205	T10206	amod	Elongated,mitochondria
R6423	T10206	T10211	nsubjpass	mitochondria,observed
R6424	T10207	T10206	amod	homozygous,mitochondria
R6425	T10208	T10206	nmod	CbCln3Δex7,mitochondria
R6426	T10209	T10208	punct	/,CbCln3Δex7
R6427	T10210	T10208	nummod	8,CbCln3Δex7
R6428	T10212	T10211	auxpass	were,observed
R6429	T10213	T10211	advmod	also,observed
R643	T1098	T1093	dobj	lines,established
R6430	T10214	T10211	prep	by,observed
R6431	T10215	T10216	compound	TEM,analysis
R6432	T10216	T10214	pobj	analysis,by
R6433	T10217	T10211	punct	.,observed
R6434	T10219	T10220	nummod	60,magnification
R6435	T10221	T10219	punct	×,60
R6436	T10222	T10220	punct	.,magnification
R6437	T10224	T10225	meta	b,shown
R6438	T10226	T10224	punct	.,b
R6439	T10227	T10228	amod	Cellular,ATP
R644	T1099	T1098	amod	neuronal,lines
R6440	T10228	T10229	compound	ATP,levels
R6441	T10229	T10225	nsubjpass	levels,shown
R6442	T10230	T10229	prep	in,levels
R6443	T10231	T10232	amod	wild,type
R6444	T10232	T10234	nmod	type,cells
R6445	T10233	T10232	punct	-,type
R6446	T10234	T10230	pobj	cells,in
R6447	T10235	T10232	punct	", ",type
R6448	T10236	T10232	conj	heterozygous,type
R6449	T10237	T10236	cc	and,heterozygous
R645	T1100	T1098	compound	precursor,lines
R6450	T10238	T10236	conj	homozygous,heterozygous
R6451	T10239	T10234	nmod	CbCln3Δex7,cells
R6452	T10240	T10239	punct	/,CbCln3Δex7
R6453	T10241	T10239	nummod	8,CbCln3Δex7
R6454	T10242	T10234	compound	precursor,cells
R6455	T10243	T10225	auxpass	are,shown
R6456	T10244	T10225	punct	.,shown
R6457	T10246	T10247	amod	Wild,type
R6458	T10247	T10249	nmod	type,cells
R6459	T10248	T10247	punct	-,type
R646	T1101	T1098	compound	cell,lines
R6460	T10249	T10263	nsubj	cells,contained
R6461	T10250	T10251	punct	(,bar
R6462	T10251	T10247	parataxis	bar,type
R6463	T10252	T10251	amod	open,bar
R6464	T10253	T10251	punct	),bar
R6465	T10254	T10247	cc	and,type
R6466	T10255	T10247	conj	heterozygous,type
R6467	T10256	T10257	punct	(,bar
R6468	T10257	T10255	parataxis	bar,heterozygous
R6469	T10258	T10257	amod	gray,bar
R647	T1102	T1093	prep	from,established
R6470	T10259	T10257	punct	),bar
R6471	T10260	T10249	nmod	CbCln3Δex7,cells
R6472	T10261	T10260	punct	/,CbCln3Δex7
R6473	T10262	T10260	nummod	8,CbCln3Δex7
R6474	T10264	T10265	punct	~,39
R6475	T10265	T10266	nummod	39,μM
R6476	T10266	T10267	compound	μM,ATP
R6477	T10267	T10263	dobj	ATP,contained
R6478	T10268	T10263	punct	", ",contained
R6479	T10269	T10270	mark	while,contained
R648	T1103	T1104	nmod	Cln3Δex7,mice
R6480	T10270	T10263	advcl	contained,contained
R6481	T10271	T10272	amod	homozygous,cells
R6482	T10272	T10270	nsubj	cells,contained
R6483	T10273	T10272	nmod	CbCln3Δex7,cells
R6484	T10274	T10273	punct	/,CbCln3Δex7
R6485	T10275	T10273	nummod	8,CbCln3Δex7
R6486	T10276	T10277	punct	(,bar
R6487	T10277	T10272	parataxis	bar,cells
R6488	T10278	T10277	amod	black,bar
R6489	T10279	T10277	punct	),bar
R649	T1104	T1102	pobj	mice,from
R6490	T10280	T10281	punct	~,1.3
R6491	T10281	T10282	npadvmod	1.3,reduced
R6492	T10282	T10284	amod	reduced,levels
R6493	T10283	T10281	advmod	fold,1.3
R6494	T10284	T10270	dobj	levels,contained
R6495	T10285	T10284	prep	of,levels
R6496	T10286	T10285	pobj	ATP,of
R6497	T10287	T10284	punct	(,levels
R6498	T10288	T10289	punct	~,30
R6499	T10289	T10290	nummod	30,μM
R65	T243	T244	nsubj	We,generated
R650	T1105	T1103	punct	/,Cln3Δex7
R6500	T10290	T10284	appos	μM,levels
R6501	T10291	T10284	punct	),levels
R6502	T10292	T10284	punct	", ",levels
R6503	T10293	T10294	dep	which,was
R6504	T10294	T10284	relcl	was,levels
R6505	T10295	T10296	advmod	statistically,significant
R6506	T10296	T10294	acomp	significant,was
R6507	T10297	T10294	prep	in,was
R6508	T10298	T10299	det	a,test
R6509	T10299	T10297	pobj	test,in
R651	T1106	T1103	nummod	8,Cln3Δex7
R6510	T10300	T10299	compound	t,test
R6511	T10301	T10299	punct	-,test
R6512	T10302	T10303	punct	(,0.0001
R6513	T10303	T10294	parataxis	0.0001,was
R6514	T10304	T10303	nsubj	p,0.0001
R6515	T10305	T10303	punct	<,0.0001
R6516	T10306	T10303	punct	),0.0001
R6517	T10307	T10263	punct	.,contained
R6518	T10309	T10310	amod	Wild,type
R6519	T10310	T10312	nmod	type,levels
R652	T1107	T1104	amod	knock,mice
R6520	T10311	T10310	punct	-,type
R6521	T10312	T10320	nsubj	levels,were
R6522	T10313	T10310	cc	and,type
R6523	T10314	T10310	conj	heterozygous,type
R6524	T10315	T10316	nmod	CbCln3Δex7,8
R6525	T10316	T10312	nmod	8,levels
R6526	T10317	T10316	punct	/,8
R6527	T10318	T10319	amod	cellular,ATP
R6528	T10319	T10312	compound	ATP,levels
R6529	T10321	T10320	neg	not,were
R653	T1108	T1107	punct	-,knock
R6530	T10322	T10323	advmod	statistically,different
R6531	T10323	T10320	acomp	different,were
R6532	T10324	T10323	prep	from,different
R6533	T10325	T10326	det	each,other
R6534	T10326	T10324	pobj	other,from
R6535	T10327	T10328	punct	(,0.4
R6536	T10328	T10320	parataxis	0.4,were
R6537	T10329	T10328	nsubj	p,0.4
R6538	T10330	T10328	punct	>,0.4
R6539	T10331	T10328	punct	),0.4
R654	T1109	T1107	prt	in,knock
R6540	T10332	T10320	punct	.,were
R6541	T10334	T10335	det	A,representative
R6542	T10335	T10336	nsubjpass	representative,shown
R6543	T10337	T10335	prep	of,representative
R6544	T10338	T10339	amod	triplicate,experiments
R6545	T10339	T10337	pobj	experiments,of
R6546	T10340	T10336	auxpass	is,shown
R6547	T10341	T10342	punct	(,6
R6548	T10342	T10336	parataxis	6,shown
R6549	T10343	T10342	nsubj	n,6
R655	T1110	T1093	punct	.,established
R6550	T10344	T10342	punct	=,6
R6551	T10345	T10342	prep	in,6
R6552	T10346	T10347	det	each,experiment
R6553	T10347	T10345	pobj	experiment,in
R6554	T10348	T10342	punct	),6
R6555	T10349	T10336	punct	.,shown
R6556	T10351	T10352	meta	c,shown
R6557	T10353	T10351	punct	.,c
R6558	T10354	T10355	compound	Cell,survival
R6559	T10355	T10352	nsubjpass	survival,shown
R656	T1112	T1113	amod	Homozygous,cells
R6560	T10356	T10355	prep	following,survival
R6561	T10357	T10358	nummod	24,hour
R6562	T10358	T10360	compound	hour,treatment
R6563	T10359	T10358	punct	-,hour
R6564	T10360	T10356	pobj	treatment,following
R6565	T10361	T10362	compound	hydrogen,peroxide
R6566	T10362	T10360	compound	peroxide,treatment
R6567	T10363	T10352	auxpass	is,shown
R6568	T10364	T10352	punct	.,shown
R6569	T10366	T10367	amod	Homozygous,cells
R657	T1113	T1117	nsubj	cells,exhibit
R6570	T10367	T10371	nsubj	cells,were
R6571	T10368	T10367	nmod	CbCln3Δex7,cells
R6572	T10369	T10368	punct	/,CbCln3Δex7
R6573	T10370	T10368	nummod	8,CbCln3Δex7
R6574	T10372	T10373	punct	~,2-fold
R6575	T10373	T10374	advmod	2-fold,more
R6576	T10374	T10375	advmod	more,sensitive
R6577	T10375	T10371	acomp	sensitive,were
R6578	T10376	T10375	prep	to,sensitive
R6579	T10377	T10378	amod	oxidative,stress
R658	T1114	T1113	nmod	CbCln3Δex7,cells
R6580	T10378	T10376	pobj	stress,to
R6581	T10379	T10378	prep	by,stress
R6582	T10380	T10381	compound	hydrogen,peroxide
R6583	T10381	T10382	compound	peroxide,treatment
R6584	T10382	T10379	pobj	treatment,by
R6585	T10383	T10371	punct	.,were
R6586	T10385	T10386	amod	Wild,type
R6587	T10386	T10388	nmod	type,cells
R6588	T10387	T10386	punct	-,type
R6589	T10388	T10400	nsubj	cells,exhibited
R659	T1115	T1114	punct	/,CbCln3Δex7
R6590	T10389	T10390	punct	(,circle
R6591	T10390	T10386	parataxis	circle,type
R6592	T10391	T10390	punct	),circle
R6593	T10392	T10386	cc	and,type
R6594	T10393	T10386	conj	heterozygous,type
R6595	T10394	T10395	punct	(,triangle
R6596	T10395	T10393	parataxis	triangle,heterozygous
R6597	T10396	T10395	punct	),triangle
R6598	T10397	T10388	nmod	CbCln3Δex7,cells
R6599	T10398	T10397	punct	/,CbCln3Δex7
R66	T245	T244	advmod	previously,generated
R660	T1116	T1114	nummod	8,CbCln3Δex7
R6600	T10399	T10397	nummod	8,CbCln3Δex7
R6601	T10401	T10402	punct	~,50
R6602	T10402	T10403	nummod	50,%
R6603	T10403	T10404	compound	%,rates
R6604	T10404	T10400	dobj	rates,exhibited
R6605	T10405	T10404	compound	survival,rates
R6606	T10406	T10404	prep	with,rates
R6607	T10407	T10408	quantmod	75,100
R6608	T10408	T10410	nummod	100,μM
R6609	T10409	T10408	punct	–,100
R661	T1118	T1119	amod	pathological,hallmarks
R6610	T10410	T10411	compound	μM,H2O2
R6611	T10411	T10406	pobj	H2O2,with
R6612	T10412	T10400	punct	", ",exhibited
R6613	T10413	T10414	mark	whereas,had
R6614	T10414	T10400	advcl	had,exhibited
R6615	T10415	T10416	amod	homozygous,cells
R6616	T10416	T10414	nsubj	cells,had
R6617	T10417	T10416	nmod	CbCln3Δex7,cells
R6618	T10418	T10417	punct	/,CbCln3Δex7
R6619	T10419	T10417	nummod	8,CbCln3Δex7
R662	T1119	T1117	dobj	hallmarks,exhibit
R6620	T10420	T10421	punct	(,squares
R6621	T10421	T10416	parataxis	squares,cells
R6622	T10422	T10421	punct	),squares
R6623	T10423	T10424	det	a,rate
R6624	T10424	T10414	dobj	rate,had
R6625	T10425	T10426	punct	~,50
R6626	T10426	T10427	nummod	50,%
R6627	T10427	T10424	compound	%,rate
R6628	T10428	T10424	compound	survival,rate
R6629	T10429	T10424	prep	with,rate
R663	T1120	T1119	prep	of,hallmarks
R6630	T10430	T10431	nummod	50,μM
R6631	T10431	T10432	compound	μM,H2O2
R6632	T10432	T10429	pobj	H2O2,with
R6633	T10433	T10400	punct	.,exhibited
R6634	T10435	T10436	det	A,representative
R6635	T10436	T10437	nsubjpass	representative,shown
R6636	T10438	T10436	prep	of,representative
R6637	T10439	T10440	amod	triplicate,experiments
R6638	T10440	T10438	pobj	experiments,of
R6639	T10441	T10437	auxpass	is,shown
R664	T1121	T1122	det	the,disease
R6640	T10442	T10443	punct	(,4
R6641	T10443	T10437	parataxis	4,shown
R6642	T10444	T10443	nsubj	n,4
R6643	T10445	T10443	punct	=,4
R6644	T10446	T10443	prep	in,4
R6645	T10447	T10448	det	each,experiment
R6646	T10448	T10446	pobj	experiment,in
R6647	T10449	T10443	punct	),4
R6648	T10450	T10437	punct	.,shown
R665	T1122	T1120	pobj	disease,of
R666	T1123	T1117	punct	", ",exhibit
R667	T1124	T1117	cc	and,exhibit
R668	T1125	T1126	det	a,survey
R669	T1126	T1127	nsubj	survey,revealed
R67	T246	T247	nmod	Cln3Δex7,mice
R670	T1127	T1117	conj	revealed,exhibit
R671	T1128	T1126	prep	of,survey
R672	T1129	T1130	compound	membrane,organelles
R673	T1130	T1128	pobj	organelles,of
R674	T1131	T1132	npadvmod	membrane,trafficking
R675	T1132	T1133	amod	trafficking,defects
R676	T1133	T1127	dobj	defects,revealed
R677	T1134	T1133	cc	and,defects
R678	T1135	T1136	amod	mitochondrial,dysfunction
R679	T1136	T1133	conj	dysfunction,defects
R68	T247	T244	dobj	mice,generated
R680	T1137	T1127	prep	in,revealed
R681	T1138	T1139	amod	homozygous,mutant
R682	T1139	T1140	nmod	mutant,cells
R683	T1140	T1137	pobj	cells,in
R684	T1141	T1140	nmod	CbCln3Δex7,cells
R685	T1142	T1141	punct	/,CbCln3Δex7
R686	T1143	T1141	nummod	8,CbCln3Δex7
R687	T1144	T1127	punct	.,revealed
R688	T1272	T1271	prep	of,Generation
R689	T1273	T1274	det	a,model
R69	T248	T246	punct	/,Cln3Δex7
R690	T1274	T1272	pobj	model,of
R691	T1275	T1276	advmod	genetically,precise
R692	T1276	T1274	amod	precise,model
R693	T1277	T1274	amod	cerebellar,model
R694	T1278	T1274	compound	JNCL,model
R695	T1279	T1274	compound	cell,model
R696	T1281	T1282	aux	To,generate
R697	T1282	T1283	advcl	generate,immortalized
R698	T1284	T1285	det	a,system
R699	T1285	T1282	dobj	system,generate
R7	T176	T171	conj	abnormalities,trafficking
R70	T249	T246	nummod	8,Cln3Δex7
R700	T1286	T1285	amod	precise,system
R701	T1287	T1285	amod	genetic,system
R702	T1288	T1285	punct	", ",system
R703	T1289	T1290	npadvmod	neuron,derived
R704	T1290	T1285	amod	derived,system
R705	T1291	T1290	punct	-,derived
R706	T1292	T1293	compound	JNCL,culture
R707	T1293	T1285	compound	culture,system
R708	T1294	T1293	compound	cell,culture
R709	T1295	T1283	punct	", ",immortalized
R71	T250	T247	amod	knock,mice
R710	T1296	T1283	nsubj	we,immortalized
R711	T1297	T1298	compound	granule,neurons
R712	T1298	T1283	dobj	neurons,immortalized
R713	T1299	T1298	acl	cultured,neurons
R714	T1300	T1299	prep	from,cultured
R715	T1301	T1302	amod	postnatal,day
R716	T1302	T1303	nmod	day,cerebella
R717	T1303	T1300	pobj	cerebella,from
R718	T1304	T1302	nummod	4,day
R719	T1305	T1302	punct	(,day
R72	T251	T250	punct	-,knock
R720	T1306	T1302	appos	P4,day
R721	T1307	T1303	punct	),cerebella
R722	T1308	T1303	prep	of,cerebella
R723	T1309	T1310	amod	homozygous,mice
R724	T1310	T1308	pobj	mice,of
R725	T1311	T1309	cc	and,homozygous
R726	T1312	T1309	conj	heterozygous,homozygous
R727	T1313	T1310	nmod	Cln3Δex7,mice
R728	T1314	T1313	punct	/,Cln3Δex7
R729	T1315	T1313	nummod	8,Cln3Δex7
R73	T252	T250	prt	in,knock
R730	T1316	T1310	amod	knock,mice
R731	T1317	T1316	punct	-,knock
R732	T1318	T1316	prt	in,knock
R733	T1319	T1310	punct	", ",mice
R734	T1320	T1310	cc	and,mice
R735	T1321	T1322	amod	wild,type
R736	T1322	T1324	compound	type,littermates
R737	T1323	T1322	punct	-,type
R738	T1324	T1310	conj	littermates,mice
R739	T1325	T1283	punct	.,immortalized
R74	T253	T247	punct	", ",mice
R740	T1327	T1328	amod	Primary,cultures
R741	T1328	T1330	nsubjpass	cultures,transduced
R742	T1329	T1328	compound	cell,cultures
R743	T1331	T1328	acl	enriched,cultures
R744	T1332	T1331	prep	for,enriched
R745	T1333	T1334	compound	granule,neurons
R746	T1334	T1332	pobj	neurons,for
R747	T1335	T1330	auxpass	were,transduced
R748	T1336	T1330	prep	with,transduced
R749	T1337	T1338	amod	retroviral,vector
R75	T254	T255	dep	which,replicate
R750	T1338	T1336	pobj	vector,with
R751	T1339	T1338	acl	bearing,vector
R752	T1340	T1341	det	a,cassette
R753	T1341	T1339	dobj	cassette,bearing
R754	T1342	T1341	compound	selection,cassette
R755	T1343	T1341	cc	and,cassette
R756	T1344	T1345	npadvmod	temperature,sensitive
R757	T1345	T1347	amod	sensitive,antigen
R758	T1346	T1345	punct	-,sensitive
R759	T1347	T1341	conj	antigen,cassette
R76	T255	T247	relcl	replicate,mice
R760	T1348	T1349	nmod	tsA58,SV40
R761	T1349	T1347	nmod	SV40,antigen
R762	T1350	T1347	amod	large,antigen
R763	T1351	T1347	compound	T,antigen
R764	T1352	T1330	punct	.,transduced
R765	T1354	T1355	nsubj	Growth,allowed
R766	T1356	T1354	prep	in,Growth
R767	T1357	T1358	npadvmod	G418,containing
R768	T1358	T1359	amod	containing,medium
R769	T1359	T1356	pobj	medium,in
R77	T256	T257	det	the,mutation
R770	T1360	T1354	prep	at,Growth
R771	T1361	T1362	det	the,temperature
R772	T1362	T1360	pobj	temperature,at
R773	T1363	T1362	amod	permissive,temperature
R774	T1364	T1362	punct	(,temperature
R775	T1365	T1366	nummod	33,°C
R776	T1366	T1362	appos	°C,temperature
R777	T1367	T1355	punct	),allowed
R778	T1368	T1355	prep	for,allowed
R779	T1369	T1368	pobj	selection,for
R78	T257	T255	dobj	mutation,replicate
R780	T1370	T1369	cc	and,selection
R781	T1371	T1369	conj	isolation,selection
R782	T1372	T1369	prep	of,selection
R783	T1373	T1374	amod	multiple,lines
R784	T1374	T1372	pobj	lines,of
R785	T1375	T1376	amod	clonal,nestin
R786	T1376	T1377	npadvmod	nestin,positive
R787	T1377	T1374	amod	positive,lines
R788	T1378	T1377	punct	-,positive
R789	T1379	T1380	punct	(,1a
R79	T258	T257	amod	common,mutation
R790	T1380	T1377	parataxis	1a,positive
R791	T1381	T1380	compound	Fig.,1a
R792	T1382	T1380	punct	),1a
R793	T1383	T1377	punct	", ",positive
R794	T1384	T1377	cc	and,positive
R795	T1385	T1386	npadvmod	GFAP,negative
R796	T1386	T1377	conj	negative,positive
R797	T1387	T1386	punct	-,negative
R798	T1388	T1389	punct	(,1b
R799	T1389	T1386	parataxis	1b,negative
R8	T177	T178	compound	subunit,c
R80	T259	T257	compound	JNCL,mutation
R800	T1390	T1389	compound	Fig.,1b
R801	T1391	T1389	punct	),1b
R802	T1392	T1374	punct	", ",lines
R803	T1393	T1374	compound	cell,lines
R804	T1394	T1374	prep	for,lines
R805	T1395	T1396	det	each,genotype
R806	T1396	T1394	pobj	genotype,for
R807	T1397	T1355	punct	.,allowed
R808	T1399	T1400	det	No,differences
R809	T1400	T1403	nsubjpass	differences,observed
R81	T260	T255	punct	", ",replicate
R810	T1401	T1400	nmod	genotype,differences
R811	T1402	T1400	amod	specific,differences
R812	T1404	T1403	auxpass	were,observed
R813	T1405	T1403	prep	in,observed
R814	T1406	T1407	amod	cellular,morphology
R815	T1407	T1405	pobj	morphology,in
R816	T1408	T1407	cc	or,morphology
R817	T1409	T1410	compound	doubling,time
R818	T1410	T1407	conj	time,morphology
R819	T1411	T1410	punct	(,time
R82	T261	T255	conj	express,replicate
R820	T1412	T1413	punct	~,46
R821	T1413	T1414	nummod	46,hours
R822	T1414	T1410	appos	hours,time
R823	T1415	T1403	punct	),observed
R824	T1416	T1417	punct	(,shown
R825	T1417	T1403	parataxis	shown,observed
R826	T1418	T1417	nsubj	data,shown
R827	T1419	T1417	neg	not,shown
R828	T1420	T1417	punct	),shown
R829	T1421	T1403	punct	.,observed
R83	T262	T263	compound	mutant,battenin
R830	T1423	T1424	mark	As,expected
R831	T1424	T1425	advcl	expected,lost
R832	T1426	T1425	punct	", ",lost
R833	T1427	T1428	nmod	SV40,expression
R834	T1428	T1425	nsubjpass	expression,lost
R835	T1429	T1428	amod	large,expression
R836	T1430	T1431	compound	T,antigen
R837	T1431	T1428	compound	antigen,expression
R838	T1432	T1425	auxpass	was,lost
R839	T1433	T1425	advmod	rapidly,lost
R84	T263	T261	dobj	battenin,express
R840	T1434	T1425	cc	and,lost
R841	T1435	T1436	compound	cell,division
R842	T1436	T1437	nsubj	division,ceased
R843	T1437	T1425	conj	ceased,lost
R844	T1438	T1439	advmod	when,shifted
R845	T1439	T1437	advcl	shifted,ceased
R846	T1440	T1439	nsubjpass	cells,shifted
R847	T1441	T1439	auxpass	were,shifted
R848	T1442	T1439	prep	to,shifted
R849	T1443	T1444	det	the,temperature
R85	T264	T261	cc	and,express
R850	T1444	T1442	pobj	temperature,to
R851	T1445	T1444	amod	non-permissive,temperature
R852	T1446	T1444	punct	(,temperature
R853	T1447	T1448	nummod	39,°C
R854	T1448	T1444	appos	°C,temperature
R855	T1449	T1439	punct	),shifted
R856	T1450	T1451	punct	(,shown
R857	T1451	T1439	parataxis	shown,shifted
R858	T1452	T1451	nsubj	data,shown
R859	T1453	T1451	neg	not,shown
R86	T265	T261	conj	display,express
R860	T1454	T1451	punct	),shown
R861	T1455	T1437	punct	.,ceased
R862	T1457	T1458	prep	Upon,became
R863	T1459	T1457	pobj	addition,Upon
R864	T1460	T1459	prep	of,addition
R865	T1461	T1462	amod	neuronal,differentiation
R866	T1462	T1463	compound	differentiation,cocktail
R867	T1463	T1460	pobj	cocktail,of
R868	T1464	T1458	punct	", ",became
R869	T1465	T1466	compound	precursor,cells
R87	T266	T267	npadvmod	JNCL,like
R870	T1466	T1458	nsubj	cells,became
R871	T1467	T1468	npadvmod	neuron,like
R872	T1468	T1458	acomp	like,became
R873	T1469	T1468	punct	-,like
R874	T1470	T1458	prep	in,became
R875	T1471	T1470	pobj	morphology,in
R876	T1472	T1458	cc	and,became
R877	T1473	T1458	conj	exhibited,became
R878	T1474	T1475	amod	decreased,expression
R879	T1475	T1473	dobj	expression,exhibited
R88	T267	T269	amod	like,pathology
R880	T1476	T1475	compound	nestin,expression
R881	T1477	T1478	punct	(,shown
R882	T1478	T1475	parataxis	shown,expression
R883	T1479	T1478	nsubj	data,shown
R884	T1480	T1478	neg	not,shown
R885	T1481	T1478	punct	),shown
R886	T1482	T1475	cc	and,expression
R887	T1483	T1484	amod	increased,expression
R888	T1484	T1475	conj	expression,expression
R889	T1485	T1484	nmod	MAP2,expression
R89	T268	T267	punct	-,like
R890	T1486	T1485	cc	and,MAP2
R891	T1487	T1485	conj	NeuN,MAP2
R892	T1488	T1489	punct	(,1d
R893	T1489	T1484	parataxis	1d,expression
R894	T1490	T1489	nmod	Fig.,1d
R895	T1491	T1489	nmod	1c,1d
R896	T1492	T1489	punct	",",1d
R897	T1493	T1489	punct	),1d
R898	T1494	T1475	punct	", ",expression
R899	T1495	T1475	cc	but,expression
R9	T178	T179	compound	c,deposition
R90	T269	T265	dobj	pathology,display
R900	T1496	T1495	neg	not,but
R901	T1497	T1475	conj	expression,expression
R902	T1498	T1497	prep	of,expression
R903	T1499	T1500	det	the,marker
R904	T1500	T1498	pobj	marker,of
R905	T1501	T1500	compound	Purkinje,marker
R906	T1502	T1500	punct	", ",marker
R907	T1503	T1500	appos	calbindin,marker
R908	T1504	T1505	punct	(,1e
R909	T1505	T1497	parataxis	1e,expression
R91	T270	T244	punct	.,generated
R910	T1506	T1505	compound	Fig.,1e
R911	T1507	T1505	punct	),1e
R912	T1508	T1458	punct	.,became
R913	T1775	T1776	amod	Homozygous,cells
R914	T1776	T1780	nsubj	cells,express
R915	T1777	T1776	nmod	CbCln3Δex7,cells
R916	T1778	T1777	punct	/,CbCln3Δex7
R917	T1779	T1777	nummod	8,CbCln3Δex7
R918	T1781	T1782	compound	mutant,battenin
R919	T1782	T1780	dobj	battenin,express
R92	T274	T275	aux	To,elucidate
R920	T1783	T1780	cc	and,express
R921	T1784	T1780	conj	display,express
R922	T1785	T1786	npadvmod	JNCL,like
R923	T1786	T1788	amod	like,pathology
R924	T1787	T1786	punct	-,like
R925	T1788	T1784	dobj	pathology,display
R926	T1790	T1791	amod	Homozygous,cells
R927	T1791	T1796	nsubjpass	cells,examined
R928	T1792	T1793	nmod	Cb,Cln3Δex7
R929	T1793	T1791	nmod	Cln3Δex7,cells
R93	T275	T276	advcl	elucidate,used
R930	T1794	T1793	punct	/,Cln3Δex7
R931	T1795	T1793	nummod	8,Cln3Δex7
R932	T1797	T1796	auxpass	were,examined
R933	T1798	T1796	advmod	first,examined
R934	T1799	T1796	prep	for,examined
R935	T1800	T1801	npadvmod	JNCL,like
R936	T1801	T1803	amod	like,characteristics
R937	T1802	T1801	punct	-,like
R938	T1803	T1799	pobj	characteristics,for
R939	T1804	T1796	punct	.,examined
R94	T277	T278	det	the,consequences
R940	T1806	T1807	amod	Homozygous,mice
R941	T1807	T1814	nsubj	mice,express
R942	T1808	T1807	nmod	Cln3Δex7,mice
R943	T1809	T1808	punct	/,Cln3Δex7
R944	T1810	T1808	nummod	8,Cln3Δex7
R945	T1811	T1807	amod	knock,mice
R946	T1812	T1811	punct	-,knock
R947	T1813	T1811	prt	in,knock
R948	T1815	T1816	amod	multiple,variants
R949	T1816	T1814	dobj	variants,express
R95	T278	T275	dobj	consequences,elucidate
R950	T1817	T1818	compound	Cln3,splice
R951	T1818	T1816	compound	splice,variants
R952	T1819	T1818	compound	mRNA,splice
R953	T1820	T1816	cc	and,variants
R954	T1821	T1822	compound	mutant,protein
R955	T1822	T1816	conj	protein,variants
R956	T1823	T1822	compound	battenin,protein
R957	T1824	T1825	dep	that,is
R958	T1825	T1822	relcl	is,protein
R959	T1826	T1825	acomp	detectable,is
R96	T279	T278	prep	of,consequences
R960	T1827	T1825	prep	by,is
R961	T1828	T1829	compound	batp1,antibody
R962	T1829	T1830	npadvmod	antibody,recognizing
R963	T1830	T1831	amod	recognizing,epitopes
R964	T1831	T1827	pobj	epitopes,by
R965	T1832	T1833	npadvmod	C,terminal
R966	T1833	T1831	amod	terminal,epitopes
R967	T1834	T1833	punct	-,terminal
R968	T1835	T1836	punct	[,12
R969	T1836	T1814	parataxis	12,express
R97	T280	T281	det	the,mutation
R970	T1837	T1836	punct	],12
R971	T1838	T1814	punct	.,express
R972	T1840	T1841	aux	To,assess
R973	T1841	T1842	advcl	assess,performed
R974	T1843	T1844	det	this,phenotype
R975	T1844	T1841	dobj	phenotype,assess
R976	T1845	T1844	amod	molecular,phenotype
R977	T1846	T1841	prep	in,assess
R978	T1847	T1848	nmod	CbCln3Δex7,cells
R979	T1848	T1846	pobj	cells,in
R98	T281	T279	pobj	mutation,of
R980	T1849	T1847	punct	/,CbCln3Δex7
R981	T1850	T1847	nummod	8,CbCln3Δex7
R982	T1851	T1842	punct	", ",performed
R983	T1852	T1853	compound	RT,PCR
R984	T1853	T1842	nsubjpass	PCR,performed
R985	T1854	T1853	punct	-,PCR
R986	T1855	T1853	cc	and,PCR
R987	T1856	T1857	nmod	anti-battenin,immunostaining
R988	T1857	T1853	conj	immunostaining,PCR
R989	T1858	T1856	punct	(,anti-battenin
R99	T282	T281	amod	common,mutation
R990	T1859	T1856	appos	batp1,anti-battenin
R991	T1860	T1857	punct	),immunostaining
R992	T1861	T1842	auxpass	were,performed
R993	T1862	T1842	punct	.,performed
R994	T1864	T1865	mark	As,shown
R995	T1865	T1866	advcl	shown,were
R996	T1867	T1865	prep	in,shown
R997	T1868	T1867	pobj	Figure,in
R998	T1869	T1868	nummod	2,Figure
R999	T1870	T1866	punct	", ",were

PMC:539297 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

PMC:539297 JSON TXT 8 Projects