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PMC:539061 / 63750-64171 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T14008 0-2 NN denotes RT
T14010 2-3 HYPH denotes -
T14009 3-6 NN denotes PCR
T14011 6-125 sentence denotes RNA was extracted from keratinocytes or skin tissue with Trizol (Invitrogen) according to the manufacturer's protocol.
T14012 7-10 NN denotes RNA
T14014 11-14 VBD denotes was
T14013 15-24 VBN denotes extracted
T14015 25-29 IN denotes from
T14016 30-43 NNS denotes keratinocytes
T14017 44-46 CC denotes or
T14018 47-51 NN denotes skin
T14019 52-58 NN denotes tissue
T14020 59-63 IN denotes with
T14021 64-70 NN denotes Trizol
T14022 71-72 -LRB- denotes (
T14023 72-82 NNP denotes Invitrogen
T14024 82-83 -RRB- denotes )
T14025 84-93 VBG denotes according
T14026 94-96 IN denotes to
T14027 97-100 DT denotes the
T14028 101-113 NN denotes manufacturer
T14030 113-115 POS denotes 's
T14029 116-124 NN denotes protocol
T14031 124-125 . denotes .
T14032 125-208 sentence denotes cDNA was generated using oligo-dT primers and the Superscript II kit (Invitrogen).
T14033 126-130 NN denotes cDNA
T14035 131-134 VBD denotes was
T14034 135-144 VBN denotes generated
T14036 145-150 VBG denotes using
T14037 151-156 NN denotes oligo
T14039 156-157 HYPH denotes -
T14038 157-159 NN denotes dT
T14040 160-167 NNS denotes primers
T14041 168-171 CC denotes and
T14042 172-175 DT denotes the
T14044 176-187 NNP denotes Superscript
T14045 188-190 CD denotes II
T14043 191-194 NN denotes kit
T14046 195-196 -LRB- denotes (
T14047 196-206 NNP denotes Invitrogen
T14048 206-207 -RRB- denotes )
T14049 207-208 . denotes .
T14050 208-421 sentence denotes The primers used for PCR were Snail forward: 5′- CAGCTGGCCAGGCTCTCGGT-3′; Snail reverse: 5′- GCGAGGGCCTCCGGAGCA-3′; GAPDH forward 5′- CGTAGACAAAATGGTGAAGGTCGG-3′; and GAPDH reverse: 5′- AAGCAGTTGGTGGTGCAGGATG-3′.
T14051 209-212 DT denotes The
T14052 213-220 NNS denotes primers
T14054 221-225 VBN denotes used
T14055 226-229 IN denotes for
T14056 230-233 NN denotes PCR
T14053 234-238 VBD denotes were
T14057 239-244 NN denotes Snail
T14058 245-252 NN denotes forward
T14059 252-254 : denotes :
T14060 254-255 CD denotes 5
T14062 255-256 SYM denotes ′
T14063 256-257 HYPH denotes -
T14061 258-278 NN denotes CAGCTGGCCAGGCTCTCGGT
T14064 278-279 HYPH denotes -
T14065 279-280 CD denotes 3
T14066 280-281 SYM denotes ′
T14067 281-282 : denotes ;
T14068 283-288 NN denotes Snail
T14069 289-296 NN denotes reverse
T14070 296-298 : denotes :
T14071 298-299 CD denotes 5
T14073 299-300 SYM denotes ′
T14074 300-301 HYPH denotes -
T14072 302-320 NN denotes GCGAGGGCCTCCGGAGCA
T14075 320-321 HYPH denotes -
T14076 321-322 CD denotes 3
T14077 322-323 SYM denotes ′
T14078 323-324 : denotes ;
T14079 325-330 NN denotes GAPDH
T14080 331-338 NN denotes forward
T14081 339-340 CD denotes 5
T14083 340-341 SYM denotes ′
T14084 341-342 HYPH denotes -
T14082 343-367 NN denotes CGTAGACAAAATGGTGAAGGTCGG
T14085 367-368 HYPH denotes -
T14086 368-369 CD denotes 3
T14087 369-370 SYM denotes ′
T14088 370-371 : denotes ;
T14089 372-375 CC denotes and
T14090 376-381 NN denotes GAPDH
T14091 382-389 NN denotes reverse
T14092 389-391 : denotes :
T14093 391-392 CD denotes 5
T14095 392-393 SYM denotes ′
T14096 393-394 HYPH denotes -
T14094 395-417 NN denotes AAGCAGTTGGTGGTGCAGGATG
T14097 417-418 HYPH denotes -
T14098 418-419 CD denotes 3
T14099 419-420 SYM denotes ′
T14100 420-421 . denotes .
R9146 T14008 T14009 compound RT,PCR
R9147 T14010 T14009 punct -,PCR
R9148 T14012 T14013 nsubjpass RNA,extracted
R9149 T14014 T14013 auxpass was,extracted
R9150 T14015 T14013 prep from,extracted
R9151 T14016 T14015 pobj keratinocytes,from
R9152 T14017 T14016 cc or,keratinocytes
R9153 T14018 T14019 compound skin,tissue
R9154 T14019 T14016 conj tissue,keratinocytes
R9155 T14020 T14013 prep with,extracted
R9156 T14021 T14020 pobj Trizol,with
R9157 T14022 T14023 punct (,Invitrogen
R9158 T14023 T14021 parataxis Invitrogen,Trizol
R9159 T14024 T14023 punct ),Invitrogen
R9160 T14025 T14013 prep according,extracted
R9161 T14026 T14025 prep to,according
R9162 T14027 T14028 det the,manufacturer
R9163 T14028 T14029 poss manufacturer,protocol
R9164 T14029 T14026 pobj protocol,to
R9165 T14030 T14028 case 's,manufacturer
R9166 T14031 T14013 punct .,extracted
R9167 T14033 T14034 nsubjpass cDNA,generated
R9168 T14035 T14034 auxpass was,generated
R9169 T14036 T14034 advcl using,generated
R9170 T14037 T14038 compound oligo,dT
R9171 T14038 T14040 compound dT,primers
R9172 T14039 T14038 punct -,dT
R9173 T14040 T14036 dobj primers,using
R9174 T14041 T14040 cc and,primers
R9175 T14042 T14043 det the,kit
R9176 T14043 T14040 conj kit,primers
R9177 T14044 T14043 nmod Superscript,kit
R9178 T14045 T14044 nummod II,Superscript
R9179 T14046 T14047 punct (,Invitrogen
R9180 T14047 T14043 parataxis Invitrogen,kit
R9181 T14048 T14047 punct ),Invitrogen
R9182 T14049 T14034 punct .,generated
R9183 T14051 T14052 det The,primers
R9184 T14052 T14053 nsubj primers,were
R9185 T14054 T14052 acl used,primers
R9186 T14055 T14054 prep for,used
R9187 T14056 T14055 pobj PCR,for
R9188 T14057 T14058 compound Snail,forward
R9189 T14058 T14053 attr forward,were
R9190 T14059 T14058 punct : ,forward
R9191 T14060 T14061 nummod 5,CAGCTGGCCAGGCTCTCGGT
R9192 T14061 T14058 appos CAGCTGGCCAGGCTCTCGGT,forward
R9193 T14062 T14060 punct ′,5
R9194 T14063 T14061 punct -,CAGCTGGCCAGGCTCTCGGT
R9195 T14064 T14061 punct -,CAGCTGGCCAGGCTCTCGGT
R9196 T14065 T14061 nummod 3,CAGCTGGCCAGGCTCTCGGT
R9197 T14066 T14061 punct ′,CAGCTGGCCAGGCTCTCGGT
R9198 T14067 T14058 punct ;,forward
R9199 T14068 T14069 compound Snail,reverse
R9200 T14069 T14058 conj reverse,forward
R9201 T14070 T14069 punct : ,reverse
R9202 T14071 T14072 nummod 5,GCGAGGGCCTCCGGAGCA
R9203 T14072 T14069 appos GCGAGGGCCTCCGGAGCA,reverse
R9204 T14073 T14071 punct ′,5
R9205 T14074 T14072 punct -,GCGAGGGCCTCCGGAGCA
R9206 T14075 T14072 punct -,GCGAGGGCCTCCGGAGCA
R9207 T14076 T14072 nummod 3,GCGAGGGCCTCCGGAGCA
R9208 T14077 T14072 punct ′,GCGAGGGCCTCCGGAGCA
R9209 T14078 T14069 punct ;,reverse
R9210 T14079 T14080 compound GAPDH,forward
R9211 T14080 T14069 conj forward,reverse
R9212 T14081 T14082 nummod 5,CGTAGACAAAATGGTGAAGGTCGG
R9213 T14082 T14080 appos CGTAGACAAAATGGTGAAGGTCGG,forward
R9214 T14083 T14081 punct ′,5
R9215 T14084 T14082 punct -,CGTAGACAAAATGGTGAAGGTCGG
R9216 T14085 T14082 punct -,CGTAGACAAAATGGTGAAGGTCGG
R9217 T14086 T14082 nummod 3,CGTAGACAAAATGGTGAAGGTCGG
R9218 T14087 T14082 punct ′,CGTAGACAAAATGGTGAAGGTCGG
R9219 T14088 T14080 punct ;,forward
R9220 T14089 T14080 cc and,forward
R9221 T14090 T14091 compound GAPDH,reverse
R9222 T14091 T14080 conj reverse,forward
R9223 T14092 T14091 punct : ,reverse
R9224 T14093 T14094 nummod 5,AAGCAGTTGGTGGTGCAGGATG
R9225 T14094 T14091 appos AAGCAGTTGGTGGTGCAGGATG,reverse
R9226 T14095 T14093 punct ′,5
R9227 T14096 T14094 punct -,AAGCAGTTGGTGGTGCAGGATG
R9228 T14097 T14094 punct -,AAGCAGTTGGTGGTGCAGGATG
R9229 T14098 T14094 nummod 3,AAGCAGTTGGTGGTGCAGGATG
R9230 T14099 T14094 punct ′,AAGCAGTTGGTGGTGCAGGATG
R9231 T14100 T14053 punct .,were