PMC:539061 / 1627-64171 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T1019	13-22	JJ	denotes	Mammalian
T1020	23-34	NN	denotes	development
T1021	35-43	VBZ	denotes	involves
T1022	44-47	DT	denotes	the
T1023	48-61	NN	denotes	morphogenesis
T1024	62-64	IN	denotes	of
T1025	65-72	JJ	denotes	complex
T1027	73-78	CD	denotes	three
T1029	78-79	HYPH	denotes	-
T1028	79-90	JJ	denotes	dimensional
T1026	91-101	NNS	denotes	structures
T1030	102-106	IN	denotes	from
T1031	107-116	RB	denotes	seemingly
T1032	117-124	JJ	denotes	uniform
T1033	125-131	NNS	denotes	sheets
T1034	132-134	CC	denotes	or
T1035	135-141	NNS	denotes	masses
T1036	142-144	IN	denotes	of
T1037	145-150	NNS	denotes	cells
T1038	150-151	.	denotes	.
T1039	151-289	sentence	denotes	A simple bud-like structure initiates the formation of many organs, including lungs, spinal cord, mammary glands, and hair follicles [1].
T1040	152-153	DT	denotes	A
T1042	154-160	JJ	denotes	simple
T1043	161-164	NN	denotes	bud
T1045	164-165	HYPH	denotes	-
T1044	165-169	JJ	denotes	like
T1041	170-179	NN	denotes	structure
T1046	180-189	VBZ	denotes	initiates
T1047	190-193	DT	denotes	the
T1048	194-203	NN	denotes	formation
T1049	204-206	IN	denotes	of
T1050	207-211	JJ	denotes	many
T1051	212-218	NNS	denotes	organs
T1052	218-220	,	denotes	,
T1053	220-229	VBG	denotes	including
T1054	230-235	NNS	denotes	lungs
T1055	235-237	,	denotes	,
T1056	237-243	JJ	denotes	spinal
T1057	244-248	NN	denotes	cord
T1058	248-250	,	denotes	,
T1059	250-257	JJ	denotes	mammary
T1060	258-264	NNS	denotes	glands
T1061	264-266	,	denotes	,
T1062	266-269	CC	denotes	and
T1063	270-274	NN	denotes	hair
T1064	275-284	NNS	denotes	follicles
T1065	285-286	-LRB-	denotes	[
T1066	286-287	CD	denotes	1
T1067	287-288	-RRB-	denotes	]
T1068	288-289	.	denotes	.
T1069	289-467	sentence	denotes	The multipotent, adhering epithelial cells are typically attached to an underlying basal lamina that polarizes the epithelial sheet and separates it from surrounding mesenchyme.
T1070	290-293	DT	denotes	The
T1072	294-305	JJ	denotes	multipotent
T1073	305-307	,	denotes	,
T1074	307-315	VBG	denotes	adhering
T1075	316-326	JJ	denotes	epithelial
T1071	327-332	NNS	denotes	cells
T1077	333-336	VBP	denotes	are
T1078	337-346	RB	denotes	typically
T1076	347-355	VBN	denotes	attached
T1079	356-358	IN	denotes	to
T1080	359-361	DT	denotes	an
T1082	362-372	VBG	denotes	underlying
T1083	373-378	JJ	denotes	basal
T1081	379-385	NN	denotes	lamina
T1084	386-390	WDT	denotes	that
T1085	391-400	VBZ	denotes	polarizes
T1086	401-404	DT	denotes	the
T1088	405-415	JJ	denotes	epithelial
T1087	416-421	NN	denotes	sheet
T1089	422-425	CC	denotes	and
T1090	426-435	VBZ	denotes	separates
T1091	436-438	PRP	denotes	it
T1092	439-443	IN	denotes	from
T1093	444-455	VBG	denotes	surrounding
T1094	456-466	NN	denotes	mesenchyme
T1095	466-467	.	denotes	.
T1096	467-647	sentence	denotes	Budding morphogenesis is guided by a reciprocal exchange of signals between epithelium and mesenchyme to specify the identity of the organ that will form and to govern its growth.
T1097	468-475	VBG	denotes	Budding
T1098	476-489	NN	denotes	morphogenesis
T1100	490-492	VBZ	denotes	is
T1099	493-499	VBN	denotes	guided
T1101	500-502	IN	denotes	by
T1102	503-504	DT	denotes	a
T1104	505-515	JJ	denotes	reciprocal
T1103	516-524	NN	denotes	exchange
T1105	525-527	IN	denotes	of
T1106	528-535	NNS	denotes	signals
T1107	536-543	IN	denotes	between
T1108	544-554	NN	denotes	epithelium
T1109	555-558	CC	denotes	and
T1110	559-569	NN	denotes	mesenchyme
T1111	570-572	TO	denotes	to
T1112	573-580	VB	denotes	specify
T1113	581-584	DT	denotes	the
T1114	585-593	NN	denotes	identity
T1115	594-596	IN	denotes	of
T1116	597-600	DT	denotes	the
T1117	601-606	NN	denotes	organ
T1118	607-611	WDT	denotes	that
T1120	612-616	MD	denotes	will
T1119	617-621	VB	denotes	form
T1121	622-625	CC	denotes	and
T1122	626-628	TO	denotes	to
T1123	629-635	VB	denotes	govern
T1124	636-639	PRP$	denotes	its
T1125	640-646	NN	denotes	growth
T1126	646-647	.	denotes	.
T1127	647-823	sentence	denotes	At the helm of these molecular communication pathways are Wnts, bone morphogenic proteins (BMPs), transforming growth factor βs (TGF-βs), and fibroblast growth factors (FGFs).
T1128	648-650	IN	denotes	At
T1130	651-654	DT	denotes	the
T1131	655-659	NN	denotes	helm
T1132	660-662	IN	denotes	of
T1133	663-668	DT	denotes	these
T1135	669-678	JJ	denotes	molecular
T1136	679-692	NN	denotes	communication
T1134	693-701	NNS	denotes	pathways
T1129	702-705	VBP	denotes	are
T1137	706-710	NNS	denotes	Wnts
T1138	710-712	,	denotes	,
T1139	712-716	NN	denotes	bone
T1141	717-728	JJ	denotes	morphogenic
T1140	729-737	NN	denotes	proteins
T1142	738-739	-LRB-	denotes	(
T1143	739-743	NNS	denotes	BMPs
T1144	743-744	-RRB-	denotes	)
T1145	744-746	,	denotes	,
T1146	746-758	VBG	denotes	transforming
T1148	759-765	NN	denotes	growth
T1149	766-772	NN	denotes	factor
T1147	773-775	NNS	denotes	βs
T1150	776-777	-LRB-	denotes	(
T1151	777-780	NN	denotes	TGF
T1153	780-781	HYPH	denotes	-
T1152	781-783	NNS	denotes	βs
T1154	783-784	-RRB-	denotes	)
T1155	784-786	,	denotes	,
T1156	786-789	CC	denotes	and
T1157	790-800	NN	denotes	fibroblast
T1159	801-807	NN	denotes	growth
T1158	808-815	NNS	denotes	factors
T1160	816-817	-LRB-	denotes	(
T1161	817-821	NNS	denotes	FGFs
T1162	821-822	-RRB-	denotes	)
T1163	822-823	.	denotes	.
T1164	823-1047	sentence	denotes	Through activation of cell surface transmembrane receptors, these external signaling molecules trigger distinct cascades of intracellular events that culminate in changes in gene expression, growth, and differentiation [2].
T1165	824-831	IN	denotes	Through
T1167	832-842	NN	denotes	activation
T1168	843-845	IN	denotes	of
T1169	846-850	NN	denotes	cell
T1170	851-858	NN	denotes	surface
T1172	859-872	NN	denotes	transmembrane
T1171	873-882	NNS	denotes	receptors
T1173	882-884	,	denotes	,
T1174	884-889	DT	denotes	these
T1176	890-898	JJ	denotes	external
T1177	899-908	NN	denotes	signaling
T1175	909-918	NNS	denotes	molecules
T1166	919-926	VBP	denotes	trigger
T1178	927-935	JJ	denotes	distinct
T1179	936-944	NNS	denotes	cascades
T1180	945-947	IN	denotes	of
T1181	948-961	JJ	denotes	intracellular
T1182	962-968	NNS	denotes	events
T1183	969-973	WDT	denotes	that
T1184	974-983	VBP	denotes	culminate
T1185	984-986	IN	denotes	in
T1186	987-994	NNS	denotes	changes
T1187	995-997	IN	denotes	in
T1188	998-1002	NN	denotes	gene
T1189	1003-1013	NN	denotes	expression
T1190	1013-1015	,	denotes	,
T1191	1015-1021	NN	denotes	growth
T1192	1021-1023	,	denotes	,
T1193	1023-1026	CC	denotes	and
T1194	1027-1042	NN	denotes	differentiation
T1195	1043-1044	-LRB-	denotes	[
T1196	1044-1045	CD	denotes	2
T1197	1045-1046	-RRB-	denotes	]
T1198	1046-1047	.	denotes	.
T1199	1047-1220	sentence	denotes	How this constellation of signals collaborates in tailoring each budding process so that it executes a distinct morphogenetic program has yet to be comprehensively defined.
T1200	1048-1051	WRB	denotes	How
T1202	1052-1056	DT	denotes	this
T1203	1057-1070	NN	denotes	constellation
T1204	1071-1073	IN	denotes	of
T1205	1074-1081	NNS	denotes	signals
T1201	1082-1094	VBZ	denotes	collaborates
T1207	1095-1097	IN	denotes	in
T1208	1098-1107	VBG	denotes	tailoring
T1209	1108-1112	DT	denotes	each
T1211	1113-1120	NN	denotes	budding
T1210	1121-1128	NN	denotes	process
T1212	1129-1131	IN	denotes	so
T1214	1132-1136	IN	denotes	that
T1215	1137-1139	PRP	denotes	it
T1213	1140-1148	VBZ	denotes	executes
T1216	1149-1150	DT	denotes	a
T1218	1151-1159	JJ	denotes	distinct
T1219	1160-1173	JJ	denotes	morphogenetic
T1217	1174-1181	NN	denotes	program
T1206	1182-1185	VBZ	denotes	has
T1220	1186-1189	RB	denotes	yet
T1222	1190-1192	TO	denotes	to
T1223	1193-1195	VB	denotes	be
T1224	1196-1211	RB	denotes	comprehensively
T1221	1212-1219	VBN	denotes	defined
T1225	1219-1220	.	denotes	.
T1226	1220-1446	sentence	denotes	However, the process appears to be patterned at the initial stages of bud formation, since the relative importance of these pathways and their downstream effectors differ as buds begin to develop and cell fates are specified.
T1227	1221-1228	RB	denotes	However
T1229	1228-1230	,	denotes	,
T1230	1230-1233	DT	denotes	the
T1231	1234-1241	NN	denotes	process
T1228	1242-1249	VBZ	denotes	appears
T1232	1250-1252	TO	denotes	to
T1234	1253-1255	VB	denotes	be
T1233	1256-1265	VBN	denotes	patterned
T1235	1266-1268	IN	denotes	at
T1236	1269-1272	DT	denotes	the
T1238	1273-1280	JJ	denotes	initial
T1237	1281-1287	NNS	denotes	stages
T1239	1288-1290	IN	denotes	of
T1240	1291-1294	NN	denotes	bud
T1241	1295-1304	NN	denotes	formation
T1242	1304-1306	,	denotes	,
T1243	1306-1311	IN	denotes	since
T1245	1312-1315	DT	denotes	the
T1247	1316-1324	JJ	denotes	relative
T1246	1325-1335	NN	denotes	importance
T1248	1336-1338	IN	denotes	of
T1249	1339-1344	DT	denotes	these
T1250	1345-1353	NNS	denotes	pathways
T1251	1354-1357	CC	denotes	and
T1252	1358-1363	PRP$	denotes	their
T1254	1364-1374	JJ	denotes	downstream
T1253	1375-1384	NNS	denotes	effectors
T1244	1385-1391	VBP	denotes	differ
T1255	1392-1394	IN	denotes	as
T1257	1395-1399	NNS	denotes	buds
T1256	1400-1405	VBP	denotes	begin
T1258	1406-1408	TO	denotes	to
T1259	1409-1416	VB	denotes	develop
T1260	1417-1420	CC	denotes	and
T1261	1421-1425	NN	denotes	cell
T1262	1426-1431	NNS	denotes	fates
T1264	1432-1435	VBP	denotes	are
T1263	1436-1445	VBN	denotes	specified
T1265	1445-1446	.	denotes	.
T1266	1446-1578	sentence	denotes	The development of a bud requires a number of coordinated changes in the behavior of the targeted cells within an epithelial sheet.
T1267	1447-1450	DT	denotes	The
T1268	1451-1462	NN	denotes	development
T1270	1463-1465	IN	denotes	of
T1271	1466-1467	DT	denotes	a
T1272	1468-1471	NN	denotes	bud
T1269	1472-1480	VBZ	denotes	requires
T1273	1481-1482	DT	denotes	a
T1274	1483-1489	NN	denotes	number
T1275	1490-1492	IN	denotes	of
T1276	1493-1504	VBN	denotes	coordinated
T1277	1505-1512	NNS	denotes	changes
T1278	1513-1515	IN	denotes	in
T1279	1516-1519	DT	denotes	the
T1280	1520-1528	NN	denotes	behavior
T1281	1529-1531	IN	denotes	of
T1282	1532-1535	DT	denotes	the
T1284	1536-1544	VBN	denotes	targeted
T1283	1545-1550	NNS	denotes	cells
T1285	1551-1557	IN	denotes	within
T1286	1558-1560	DT	denotes	an
T1288	1561-1571	JJ	denotes	epithelial
T1287	1572-1577	NN	denotes	sheet
T1289	1577-1578	.	denotes	.
T1290	1578-1762	sentence	denotes	The process must be accompanied by alterations in the proliferation, polarity, shape, and adhesiveness of selected cells, as well as by modifications in their underlying basal lamina.
T1291	1579-1582	DT	denotes	The
T1292	1583-1590	NN	denotes	process
T1294	1591-1595	MD	denotes	must
T1295	1596-1598	VB	denotes	be
T1293	1599-1610	VBN	denotes	accompanied
T1296	1611-1613	IN	denotes	by
T1297	1614-1625	NNS	denotes	alterations
T1298	1626-1628	IN	denotes	in
T1299	1629-1632	DT	denotes	the
T1300	1633-1646	NN	denotes	proliferation
T1301	1646-1648	,	denotes	,
T1302	1648-1656	NN	denotes	polarity
T1303	1656-1658	,	denotes	,
T1304	1658-1663	NN	denotes	shape
T1305	1663-1665	,	denotes	,
T1306	1665-1668	CC	denotes	and
T1307	1669-1681	NN	denotes	adhesiveness
T1308	1682-1684	IN	denotes	of
T1309	1685-1693	VBN	denotes	selected
T1310	1694-1699	NNS	denotes	cells
T1311	1699-1701	,	denotes	,
T1312	1701-1703	RB	denotes	as
T1314	1704-1708	RB	denotes	well
T1313	1709-1711	IN	denotes	as
T1315	1712-1714	IN	denotes	by
T1316	1715-1728	NNS	denotes	modifications
T1317	1729-1731	IN	denotes	in
T1318	1732-1737	PRP$	denotes	their
T1320	1738-1748	VBG	denotes	underlying
T1321	1749-1754	JJ	denotes	basal
T1319	1755-1761	NN	denotes	lamina
T1322	1761-1762	.	denotes	.
T1323	1762-1990	sentence	denotes	Thus, extracellular epithelial-mesenchymal crosstalk must be intricately orchestrated to couple the determination of distinct cell fates with the contemporaneous remodeling of the physical and structural properties of the cell.
T1324	1763-1767	RB	denotes	Thus
T1326	1767-1769	,	denotes	,
T1327	1769-1782	JJ	denotes	extracellular
T1329	1783-1793	JJ	denotes	epithelial
T1331	1793-1794	HYPH	denotes	-
T1330	1794-1805	JJ	denotes	mesenchymal
T1328	1806-1815	NN	denotes	crosstalk
T1332	1816-1820	MD	denotes	must
T1333	1821-1823	VB	denotes	be
T1334	1824-1835	RB	denotes	intricately
T1325	1836-1848	VBN	denotes	orchestrated
T1335	1849-1851	TO	denotes	to
T1336	1852-1858	VB	denotes	couple
T1337	1859-1862	DT	denotes	the
T1338	1863-1876	NN	denotes	determination
T1339	1877-1879	IN	denotes	of
T1340	1880-1888	JJ	denotes	distinct
T1342	1889-1893	NN	denotes	cell
T1341	1894-1899	NNS	denotes	fates
T1343	1900-1904	IN	denotes	with
T1344	1905-1908	DT	denotes	the
T1346	1909-1924	JJ	denotes	contemporaneous
T1345	1925-1935	NN	denotes	remodeling
T1347	1936-1938	IN	denotes	of
T1348	1939-1942	DT	denotes	the
T1350	1943-1951	JJ	denotes	physical
T1351	1952-1955	CC	denotes	and
T1352	1956-1966	JJ	denotes	structural
T1349	1967-1977	NNS	denotes	properties
T1353	1978-1980	IN	denotes	of
T1354	1981-1984	DT	denotes	the
T1355	1985-1989	NN	denotes	cell
T1356	1989-1990	.	denotes	.
T1357	1990-2106	sentence	denotes	Among the few dispensable organs, hair follicles offer an excellent model system to study epithelial bud formation.
T1358	1991-1996	IN	denotes	Among
T1360	1997-2000	DT	denotes	the
T1362	2001-2004	JJ	denotes	few
T1363	2005-2016	JJ	denotes	dispensable
T1361	2017-2023	NNS	denotes	organs
T1364	2023-2025	,	denotes	,
T1365	2025-2029	NN	denotes	hair
T1366	2030-2039	NNS	denotes	follicles
T1359	2040-2045	VBP	denotes	offer
T1367	2046-2048	DT	denotes	an
T1369	2049-2058	JJ	denotes	excellent
T1370	2059-2064	NN	denotes	model
T1368	2065-2071	NN	denotes	system
T1371	2072-2074	TO	denotes	to
T1372	2075-2080	VB	denotes	study
T1373	2081-2091	JJ	denotes	epithelial
T1374	2092-2095	NN	denotes	bud
T1375	2096-2105	NN	denotes	formation
T1376	2105-2106	.	denotes	.
T1377	2106-2190	sentence	denotes	Mammalian skin epithelium begins as a single sheet of multipotent ectodermal cells.
T1378	2107-2116	JJ	denotes	Mammalian
T1380	2117-2121	NN	denotes	skin
T1379	2122-2132	NN	denotes	epithelium
T1381	2133-2139	VBZ	denotes	begins
T1382	2140-2142	IN	denotes	as
T1383	2143-2144	DT	denotes	a
T1385	2145-2151	JJ	denotes	single
T1384	2152-2157	NN	denotes	sheet
T1386	2158-2160	IN	denotes	of
T1387	2161-2172	JJ	denotes	multipotent
T1389	2173-2183	JJ	denotes	ectodermal
T1388	2184-2189	NNS	denotes	cells
T1390	2189-2190	.	denotes	.
T1391	2190-2377	sentence	denotes	During development, specialized mesenchymal cells populate the skin in a spatially defined pattern to initiate the complex epithelial-mesenchymal crosstalk that will specify the bud [3].
T1392	2191-2197	IN	denotes	During
T1394	2198-2209	NN	denotes	development
T1395	2209-2211	,	denotes	,
T1396	2211-2222	JJ	denotes	specialized
T1398	2223-2234	JJ	denotes	mesenchymal
T1397	2235-2240	NNS	denotes	cells
T1393	2241-2249	VBP	denotes	populate
T1399	2250-2253	DT	denotes	the
T1400	2254-2258	NN	denotes	skin
T1401	2259-2261	IN	denotes	in
T1402	2262-2263	DT	denotes	a
T1404	2264-2273	RB	denotes	spatially
T1405	2274-2281	VBN	denotes	defined
T1403	2282-2289	NN	denotes	pattern
T1406	2290-2292	TO	denotes	to
T1407	2293-2301	VB	denotes	initiate
T1408	2302-2305	DT	denotes	the
T1410	2306-2313	JJ	denotes	complex
T1411	2314-2324	JJ	denotes	epithelial
T1413	2324-2325	HYPH	denotes	-
T1412	2325-2336	JJ	denotes	mesenchymal
T1409	2337-2346	NN	denotes	crosstalk
T1414	2347-2351	WDT	denotes	that
T1416	2352-2356	MD	denotes	will
T1415	2357-2364	VB	denotes	specify
T1417	2365-2368	DT	denotes	the
T1418	2369-2372	NN	denotes	bud
T1419	2373-2374	-LRB-	denotes	[
T1420	2374-2375	CD	denotes	3
T1421	2375-2376	-RRB-	denotes	]
T1422	2376-2377	.	denotes	.
T1423	2377-2598	sentence	denotes	Once committed, a small cluster of epithelial cells, the placode, instructs a group of underlying mesenchymal cells to condense and form the nascent dermal papilla, which will be a permanent fixture of the hair follicle.
T1424	2378-2382	IN	denotes	Once
T1425	2383-2392	VBN	denotes	committed
T1427	2392-2394	,	denotes	,
T1428	2394-2395	DT	denotes	a
T1430	2396-2401	JJ	denotes	small
T1429	2402-2409	NN	denotes	cluster
T1431	2410-2412	IN	denotes	of
T1432	2413-2423	JJ	denotes	epithelial
T1433	2424-2429	NNS	denotes	cells
T1434	2429-2431	,	denotes	,
T1435	2431-2434	DT	denotes	the
T1436	2435-2442	NN	denotes	placode
T1437	2442-2444	,	denotes	,
T1426	2444-2453	VBZ	denotes	instructs
T1438	2454-2455	DT	denotes	a
T1439	2456-2461	NN	denotes	group
T1441	2462-2464	IN	denotes	of
T1442	2465-2475	VBG	denotes	underlying
T1444	2476-2487	JJ	denotes	mesenchymal
T1443	2488-2493	NNS	denotes	cells
T1445	2494-2496	TO	denotes	to
T1440	2497-2505	VB	denotes	condense
T1446	2506-2509	CC	denotes	and
T1447	2510-2514	VB	denotes	form
T1448	2515-2518	DT	denotes	the
T1450	2519-2526	JJ	denotes	nascent
T1451	2527-2533	JJ	denotes	dermal
T1449	2534-2541	NN	denotes	papilla
T1452	2541-2543	,	denotes	,
T1453	2543-2548	WDT	denotes	which
T1455	2549-2553	MD	denotes	will
T1454	2554-2556	VB	denotes	be
T1456	2557-2558	DT	denotes	a
T1458	2559-2568	JJ	denotes	permanent
T1457	2569-2576	NN	denotes	fixture
T1459	2577-2579	IN	denotes	of
T1460	2580-2583	DT	denotes	the
T1462	2584-2588	NN	denotes	hair
T1461	2589-2597	NN	denotes	follicle
T1463	2597-2598	.	denotes	.
T1464	2598-2835	sentence	denotes	Subsequent exchanges between the placode and nascent dermal papilla result in further growth of the follicle into the underlying dermis, or down-growth, and eventual differentiation into the six concentric layers of the mature follicle.
T1465	2599-2609	JJ	denotes	Subsequent
T1466	2610-2619	NNS	denotes	exchanges
T1468	2620-2627	IN	denotes	between
T1469	2628-2631	DT	denotes	the
T1470	2632-2639	NN	denotes	placode
T1471	2640-2643	CC	denotes	and
T1472	2644-2651	JJ	denotes	nascent
T1474	2652-2658	JJ	denotes	dermal
T1473	2659-2666	NN	denotes	papilla
T1467	2667-2673	VBP	denotes	result
T1475	2674-2676	IN	denotes	in
T1476	2677-2684	JJ	denotes	further
T1477	2685-2691	NN	denotes	growth
T1478	2692-2694	IN	denotes	of
T1479	2695-2698	DT	denotes	the
T1480	2699-2707	NN	denotes	follicle
T1481	2708-2712	IN	denotes	into
T1482	2713-2716	DT	denotes	the
T1484	2717-2727	JJ	denotes	underlying
T1483	2728-2734	NN	denotes	dermis
T1485	2734-2736	,	denotes	,
T1486	2736-2738	CC	denotes	or
T1487	2739-2743	JJ	denotes	down
T1489	2743-2744	HYPH	denotes	-
T1488	2744-2750	NN	denotes	growth
T1490	2750-2752	,	denotes	,
T1491	2752-2755	CC	denotes	and
T1492	2756-2764	JJ	denotes	eventual
T1493	2765-2780	NN	denotes	differentiation
T1494	2781-2785	IN	denotes	into
T1495	2786-2789	DT	denotes	the
T1497	2790-2793	CD	denotes	six
T1498	2794-2804	JJ	denotes	concentric
T1496	2805-2811	NNS	denotes	layers
T1499	2812-2814	IN	denotes	of
T1500	2815-2818	DT	denotes	the
T1502	2819-2825	JJ	denotes	mature
T1501	2826-2834	NN	denotes	follicle
T1503	2834-2835	.	denotes	.
T1504	2835-3105	sentence	denotes	Previously, we delineated how two respective epithelial and mesenchymal signals, Wnts and the BMP-inhibitory factor noggin, function in concert to induce lymphoid enhancer factor-1/β-catenin (LEF-1/β-catenin)-mediated gene transcription within the follicle placode [4].
T1505	2836-2846	RB	denotes	Previously
T1507	2846-2848	,	denotes	,
T1508	2848-2850	PRP	denotes	we
T1506	2851-2861	VBD	denotes	delineated
T1509	2862-2865	WRB	denotes	how
T1511	2866-2869	CD	denotes	two
T1513	2870-2880	JJ	denotes	respective
T1514	2881-2891	JJ	denotes	epithelial
T1515	2892-2895	CC	denotes	and
T1516	2896-2907	JJ	denotes	mesenchymal
T1512	2908-2915	NNS	denotes	signals
T1517	2915-2917	,	denotes	,
T1518	2917-2921	NNS	denotes	Wnts
T1519	2922-2925	CC	denotes	and
T1520	2926-2929	DT	denotes	the
T1522	2930-2933	NN	denotes	BMP
T1524	2933-2934	HYPH	denotes	-
T1523	2934-2944	JJ	denotes	inhibitory
T1521	2945-2951	NN	denotes	factor
T1525	2952-2958	NN	denotes	noggin
T1526	2958-2960	,	denotes	,
T1510	2960-2968	VBP	denotes	function
T1527	2969-2971	IN	denotes	in
T1528	2972-2979	NN	denotes	concert
T1529	2980-2982	TO	denotes	to
T1530	2983-2989	VB	denotes	induce
T1531	2990-2998	JJ	denotes	lymphoid
T1533	2999-3007	NN	denotes	enhancer
T1532	3008-3014	NN	denotes	factor
T1535	3014-3015	HYPH	denotes	-
T1536	3015-3016	CD	denotes	1
T1537	3016-3017	HYPH	denotes	/
T1538	3017-3018	NN	denotes	β
T1540	3018-3019	HYPH	denotes	-
T1539	3019-3026	NN	denotes	catenin
T1541	3027-3028	-LRB-	denotes	(
T1542	3028-3031	NN	denotes	LEF
T1543	3031-3032	HYPH	denotes	-
T1544	3032-3033	CD	denotes	1
T1545	3033-3034	HYPH	denotes	/
T1546	3034-3035	NN	denotes	β
T1548	3035-3036	HYPH	denotes	-
T1547	3036-3043	NN	denotes	catenin
T1549	3043-3044	-RRB-	denotes	)
T1550	3044-3045	HYPH	denotes	-
T1534	3045-3053	VBN	denotes	mediated
T1552	3054-3058	NN	denotes	gene
T1551	3059-3072	NN	denotes	transcription
T1553	3073-3079	IN	denotes	within
T1554	3080-3083	DT	denotes	the
T1556	3084-3092	NN	denotes	follicle
T1555	3093-3100	NN	denotes	placode
T1557	3101-3102	-LRB-	denotes	[
T1558	3102-3103	CD	denotes	4
T1559	3103-3104	-RRB-	denotes	]
T1560	3104-3105	.	denotes	.
T1561	3105-3370	sentence	denotes	The downstream changes elicited through convergence of these two early signaling pathways include down-regulation of the gene encoding E-cadherin, the prototypical epithelial cadherin that forms the transmembrane core of intercellular adherens junctions (AJs) [5].
T1562	3106-3109	DT	denotes	The
T1564	3110-3120	JJ	denotes	downstream
T1563	3121-3128	NNS	denotes	changes
T1566	3129-3137	VBN	denotes	elicited
T1567	3138-3145	IN	denotes	through
T1568	3146-3157	NN	denotes	convergence
T1569	3158-3160	IN	denotes	of
T1570	3161-3166	DT	denotes	these
T1572	3167-3170	CD	denotes	two
T1573	3171-3176	JJ	denotes	early
T1574	3177-3186	NN	denotes	signaling
T1571	3187-3195	NNS	denotes	pathways
T1565	3196-3203	VBP	denotes	include
T1575	3204-3208	JJ	denotes	down
T1577	3208-3209	HYPH	denotes	-
T1576	3209-3219	NN	denotes	regulation
T1578	3220-3222	IN	denotes	of
T1579	3223-3226	DT	denotes	the
T1580	3227-3231	NN	denotes	gene
T1581	3232-3240	VBG	denotes	encoding
T1582	3241-3242	NN	denotes	E
T1584	3242-3243	HYPH	denotes	-
T1583	3243-3251	NN	denotes	cadherin
T1585	3251-3253	,	denotes	,
T1586	3253-3256	DT	denotes	the
T1588	3257-3269	JJ	denotes	prototypical
T1589	3270-3280	JJ	denotes	epithelial
T1587	3281-3289	NN	denotes	cadherin
T1590	3290-3294	WDT	denotes	that
T1591	3295-3300	VBZ	denotes	forms
T1592	3301-3304	DT	denotes	the
T1594	3305-3318	JJ	denotes	transmembrane
T1593	3319-3323	NN	denotes	core
T1595	3324-3326	IN	denotes	of
T1596	3327-3340	JJ	denotes	intercellular
T1598	3341-3349	NN	denotes	adherens
T1597	3350-3359	NNS	denotes	junctions
T1599	3360-3361	-LRB-	denotes	(
T1600	3361-3364	NNS	denotes	AJs
T1601	3364-3365	-RRB-	denotes	)
T1602	3366-3367	-LRB-	denotes	[
T1603	3367-3368	CD	denotes	5
T1604	3368-3369	-RRB-	denotes	]
T1605	3369-3370	.	denotes	.
T1606	3370-3635	sentence	denotes	We subsequently showed that when E-cadherin is transgenically elevated in mouse skin, hair follicle morphogenesis is blocked, suggesting that E-cadherin down-regulation is a critical event in governing the adhesion dynamics necessary for budding morphogenesis [4].
T1607	3371-3373	PRP	denotes	We
T1609	3374-3386	RB	denotes	subsequently
T1608	3387-3393	VBD	denotes	showed
T1610	3394-3398	IN	denotes	that
T1612	3399-3403	WRB	denotes	when
T1614	3404-3405	NN	denotes	E
T1616	3405-3406	HYPH	denotes	-
T1615	3406-3414	NN	denotes	cadherin
T1617	3415-3417	VBZ	denotes	is
T1618	3418-3432	RB	denotes	transgenically
T1613	3433-3441	VBN	denotes	elevated
T1619	3442-3444	IN	denotes	in
T1620	3445-3450	NN	denotes	mouse
T1621	3451-3455	NN	denotes	skin
T1622	3455-3457	,	denotes	,
T1623	3457-3461	NN	denotes	hair
T1624	3462-3470	NN	denotes	follicle
T1625	3471-3484	NN	denotes	morphogenesis
T1626	3485-3487	VBZ	denotes	is
T1611	3488-3495	VBN	denotes	blocked
T1627	3495-3497	,	denotes	,
T1628	3497-3507	VBG	denotes	suggesting
T1629	3508-3512	IN	denotes	that
T1631	3513-3514	NN	denotes	E
T1633	3514-3515	HYPH	denotes	-
T1632	3515-3523	NN	denotes	cadherin
T1635	3524-3528	JJ	denotes	down
T1636	3528-3529	HYPH	denotes	-
T1634	3529-3539	NN	denotes	regulation
T1630	3540-3542	VBZ	denotes	is
T1637	3543-3544	DT	denotes	a
T1639	3545-3553	JJ	denotes	critical
T1638	3554-3559	NN	denotes	event
T1640	3560-3562	IN	denotes	in
T1641	3563-3572	VBG	denotes	governing
T1642	3573-3576	DT	denotes	the
T1644	3577-3585	NN	denotes	adhesion
T1643	3586-3594	NNS	denotes	dynamics
T1645	3595-3604	JJ	denotes	necessary
T1646	3605-3608	IN	denotes	for
T1647	3609-3616	NN	denotes	budding
T1648	3617-3630	NN	denotes	morphogenesis
T1649	3631-3632	-LRB-	denotes	[
T1650	3632-3633	CD	denotes	4
T1651	3633-3634	-RRB-	denotes	]
T1652	3634-3635	.	denotes	.
T1653	3635-3683	sentence	denotes	Like LEF-1, E-cadherin also binds to β-catenin.
T1654	3636-3640	IN	denotes	Like
T1656	3641-3644	NN	denotes	LEF
T1657	3644-3645	HYPH	denotes	-
T1658	3645-3646	CD	denotes	1
T1659	3646-3648	,	denotes	,
T1660	3648-3649	NN	denotes	E
T1662	3649-3650	HYPH	denotes	-
T1661	3650-3658	NN	denotes	cadherin
T1663	3659-3663	RB	denotes	also
T1655	3664-3669	VBZ	denotes	binds
T1664	3670-3672	IN	denotes	to
T1665	3673-3674	NN	denotes	β
T1667	3674-3675	HYPH	denotes	-
T1666	3675-3682	NN	denotes	catenin
T1668	3682-3683	.	denotes	.
T1669	3683-3946	sentence	denotes	At sites of cell-cell contact, however, E-cadherin-β-catenin complexes recruit α-catenin, which in turn coordinates the associated actin polymerization dynamics necessary to stabilize nascent AJs and integrate the cytoskeleton across an epithelial sheet [6,7,8].
T1670	3684-3686	IN	denotes	At
T1672	3687-3692	NNS	denotes	sites
T1673	3693-3695	IN	denotes	of
T1674	3696-3700	NN	denotes	cell
T1676	3700-3701	HYPH	denotes	-
T1675	3701-3705	NN	denotes	cell
T1677	3706-3713	NN	denotes	contact
T1678	3713-3715	,	denotes	,
T1679	3715-3722	RB	denotes	however
T1680	3722-3724	,	denotes	,
T1681	3724-3725	NN	denotes	E
T1683	3725-3726	HYPH	denotes	-
T1682	3726-3734	NN	denotes	cadherin
T1685	3734-3735	HYPH	denotes	-
T1686	3735-3736	NN	denotes	β
T1688	3736-3737	HYPH	denotes	-
T1687	3737-3744	NN	denotes	catenin
T1684	3745-3754	NNS	denotes	complexes
T1671	3755-3762	VBP	denotes	recruit
T1689	3763-3764	NN	denotes	α
T1691	3764-3765	HYPH	denotes	-
T1690	3765-3772	NN	denotes	catenin
T1692	3772-3774	,	denotes	,
T1693	3774-3779	WDT	denotes	which
T1695	3780-3782	IN	denotes	in
T1696	3783-3787	NN	denotes	turn
T1694	3788-3799	VBZ	denotes	coordinates
T1697	3800-3803	DT	denotes	the
T1699	3804-3814	VBN	denotes	associated
T1700	3815-3820	NN	denotes	actin
T1701	3821-3835	NN	denotes	polymerization
T1698	3836-3844	NNS	denotes	dynamics
T1702	3845-3854	JJ	denotes	necessary
T1703	3855-3857	TO	denotes	to
T1704	3858-3867	VB	denotes	stabilize
T1705	3868-3875	JJ	denotes	nascent
T1706	3876-3879	NNS	denotes	AJs
T1707	3880-3883	CC	denotes	and
T1708	3884-3893	VB	denotes	integrate
T1709	3894-3897	DT	denotes	the
T1710	3898-3910	NN	denotes	cytoskeleton
T1711	3911-3917	IN	denotes	across
T1712	3918-3920	DT	denotes	an
T1714	3921-3931	JJ	denotes	epithelial
T1713	3932-3937	NN	denotes	sheet
T1715	3938-3939	-LRB-	denotes	[
T1717	3939-3940	CD	denotes	6
T1718	3940-3941	,	denotes	,
T1719	3941-3942	CD	denotes	7
T1720	3942-3943	,	denotes	,
T1716	3943-3944	CD	denotes	8
T1721	3944-3945	-RRB-	denotes	]
T1722	3945-3946	.	denotes	.
T1723	3946-4203	sentence	denotes	α-Catenin also binds to the class III Lin-1, Isl-1, Mec-3 (LIM) protein Ajuba (a member of the zyxin family of proteins), which appears to function dually in both adhesion and in activation of the Ras-mitogen-activated protein kinase (MAPK) pathway [9,10].
T1724	3947-3948	NN	denotes	α
T1726	3948-3949	HYPH	denotes	-
T1725	3949-3956	NN	denotes	Catenin
T1728	3957-3961	RB	denotes	also
T1727	3962-3967	VBZ	denotes	binds
T1729	3968-3970	IN	denotes	to
T1730	3971-3974	DT	denotes	the
T1732	3975-3980	NN	denotes	class
T1733	3981-3984	CD	denotes	III
T1734	3985-3988	NN	denotes	Lin
T1735	3988-3989	HYPH	denotes	-
T1736	3989-3990	CD	denotes	1
T1737	3990-3992	,	denotes	,
T1738	3992-3995	NN	denotes	Isl
T1739	3995-3996	HYPH	denotes	-
T1740	3996-3997	CD	denotes	1
T1741	3997-3999	,	denotes	,
T1742	3999-4002	NN	denotes	Mec
T1743	4002-4003	HYPH	denotes	-
T1744	4003-4004	CD	denotes	3
T1745	4005-4006	-LRB-	denotes	(
T1746	4006-4009	NN	denotes	LIM
T1747	4009-4010	-RRB-	denotes	)
T1731	4011-4018	NN	denotes	protein
T1748	4019-4024	NN	denotes	Ajuba
T1749	4025-4026	-LRB-	denotes	(
T1750	4026-4027	DT	denotes	a
T1751	4028-4034	NN	denotes	member
T1752	4035-4037	IN	denotes	of
T1753	4038-4041	DT	denotes	the
T1755	4042-4047	NN	denotes	zyxin
T1754	4048-4054	NN	denotes	family
T1756	4055-4057	IN	denotes	of
T1757	4058-4066	NN	denotes	proteins
T1758	4066-4067	-RRB-	denotes	)
T1759	4067-4069	,	denotes	,
T1760	4069-4074	WDT	denotes	which
T1761	4075-4082	VBZ	denotes	appears
T1762	4083-4085	TO	denotes	to
T1763	4086-4094	VB	denotes	function
T1764	4095-4101	RB	denotes	dually
T1765	4102-4104	IN	denotes	in
T1766	4105-4109	CC	denotes	both
T1767	4110-4118	NN	denotes	adhesion
T1768	4119-4122	CC	denotes	and
T1769	4123-4125	IN	denotes	in
T1770	4126-4136	NN	denotes	activation
T1771	4137-4139	IN	denotes	of
T1772	4140-4143	DT	denotes	the
T1774	4144-4147	NN	denotes	Ras
T1775	4147-4148	HYPH	denotes	-
T1776	4148-4155	NN	denotes	mitogen
T1778	4155-4156	HYPH	denotes	-
T1777	4156-4165	VBN	denotes	activated
T1780	4166-4173	NN	denotes	protein
T1779	4174-4180	NN	denotes	kinase
T1781	4181-4182	-LRB-	denotes	(
T1782	4182-4186	NN	denotes	MAPK
T1783	4186-4187	-RRB-	denotes	)
T1773	4188-4195	NN	denotes	pathway
T1784	4196-4197	-LRB-	denotes	[
T1786	4197-4198	CD	denotes	9
T1787	4198-4199	,	denotes	,
T1785	4199-4201	CD	denotes	10
T1788	4201-4202	-RRB-	denotes	]
T1789	4202-4203	.	denotes	.
T1790	4203-4337	sentence	denotes	Through these links, AJs appear able to couple adhesion with cytoskeletal dynamics as well as with nuclear and cytoplasmic signaling.
T1791	4204-4211	IN	denotes	Through
T1793	4212-4217	DT	denotes	these
T1794	4218-4223	NNS	denotes	links
T1795	4223-4225	,	denotes	,
T1796	4225-4228	NNS	denotes	AJs
T1792	4229-4235	VBP	denotes	appear
T1797	4236-4240	JJ	denotes	able
T1798	4241-4243	TO	denotes	to
T1799	4244-4250	VB	denotes	couple
T1800	4251-4259	NN	denotes	adhesion
T1801	4260-4264	IN	denotes	with
T1802	4265-4277	JJ	denotes	cytoskeletal
T1803	4278-4286	NNS	denotes	dynamics
T1804	4287-4289	RB	denotes	as
T1806	4290-4294	RB	denotes	well
T1805	4295-4297	IN	denotes	as
T1807	4298-4302	IN	denotes	with
T1808	4303-4310	JJ	denotes	nuclear
T1810	4311-4314	CC	denotes	and
T1811	4315-4326	JJ	denotes	cytoplasmic
T1809	4327-4336	NN	denotes	signaling
T1812	4336-4337	.	denotes	.
T1813	4337-4485	sentence	denotes	This provides a framework for conceptualizing why E-cadherin levels appear to impact upon a plethora of developmental processes (reviewed in [11]).
T1814	4338-4342	DT	denotes	This
T1815	4343-4351	VBZ	denotes	provides
T1816	4352-4353	DT	denotes	a
T1817	4354-4363	NN	denotes	framework
T1818	4364-4367	IN	denotes	for
T1819	4368-4383	VBG	denotes	conceptualizing
T1820	4384-4387	WRB	denotes	why
T1822	4388-4389	NN	denotes	E
T1824	4389-4390	HYPH	denotes	-
T1823	4390-4398	NN	denotes	cadherin
T1825	4399-4405	NNS	denotes	levels
T1821	4406-4412	VBP	denotes	appear
T1826	4413-4415	TO	denotes	to
T1827	4416-4422	VB	denotes	impact
T1828	4423-4427	IN	denotes	upon
T1829	4428-4429	DT	denotes	a
T1830	4430-4438	NN	denotes	plethora
T1831	4439-4441	IN	denotes	of
T1832	4442-4455	JJ	denotes	developmental
T1833	4456-4465	NNS	denotes	processes
T1834	4466-4467	-LRB-	denotes	(
T1835	4467-4475	VBN	denotes	reviewed
T1836	4476-4478	IN	denotes	in
T1837	4479-4480	-LRB-	denotes	[
T1838	4480-4482	CD	denotes	11
T1839	4482-4483	-RRB-	denotes	]
T1840	4483-4484	-RRB-	denotes	)
T1841	4484-4485	.	denotes	.
T1842	4485-4769	sentence	denotes	As we probed more deeply into the underlying mechanisms governing E-cadherin promoter activity, we were intrigued by the close proximity of the LEF-1/β-catenin binding site to a site known to bind the Snail/Slug family of zinc finger transcriptional repressor proteins [12,13,14,15].
T1843	4486-4488	IN	denotes	As
T1845	4489-4491	PRP	denotes	we
T1844	4492-4498	VBD	denotes	probed
T1847	4499-4503	RBR	denotes	more
T1848	4504-4510	RB	denotes	deeply
T1849	4511-4515	IN	denotes	into
T1850	4516-4519	DT	denotes	the
T1852	4520-4530	JJ	denotes	underlying
T1851	4531-4541	NNS	denotes	mechanisms
T1853	4542-4551	VBG	denotes	governing
T1854	4552-4553	NN	denotes	E
T1856	4553-4554	HYPH	denotes	-
T1855	4554-4562	NN	denotes	cadherin
T1857	4563-4571	NN	denotes	promoter
T1858	4572-4580	NN	denotes	activity
T1859	4580-4582	,	denotes	,
T1860	4582-4584	PRP	denotes	we
T1861	4585-4589	VBD	denotes	were
T1846	4590-4599	VBN	denotes	intrigued
T1862	4600-4602	IN	denotes	by
T1863	4603-4606	DT	denotes	the
T1865	4607-4612	JJ	denotes	close
T1864	4613-4622	NN	denotes	proximity
T1866	4623-4625	IN	denotes	of
T1867	4626-4629	DT	denotes	the
T1869	4630-4633	NN	denotes	LEF
T1870	4633-4634	HYPH	denotes	-
T1871	4634-4635	CD	denotes	1
T1872	4635-4636	HYPH	denotes	/
T1873	4636-4637	NN	denotes	β
T1875	4637-4638	HYPH	denotes	-
T1874	4638-4645	NN	denotes	catenin
T1876	4646-4653	NN	denotes	binding
T1868	4654-4658	NN	denotes	site
T1877	4659-4661	IN	denotes	to
T1878	4662-4663	DT	denotes	a
T1879	4664-4668	NN	denotes	site
T1880	4669-4674	VBN	denotes	known
T1881	4675-4677	TO	denotes	to
T1882	4678-4682	VB	denotes	bind
T1883	4683-4686	DT	denotes	the
T1885	4687-4692	NN	denotes	Snail
T1887	4692-4693	HYPH	denotes	/
T1886	4693-4697	NN	denotes	Slug
T1884	4698-4704	NN	denotes	family
T1888	4705-4707	IN	denotes	of
T1889	4708-4712	NN	denotes	zinc
T1890	4713-4719	NN	denotes	finger
T1892	4720-4735	JJ	denotes	transcriptional
T1893	4736-4745	NN	denotes	repressor
T1891	4746-4754	NN	denotes	proteins
T1894	4755-4756	-LRB-	denotes	[
T1896	4756-4758	CD	denotes	12
T1897	4758-4759	,	denotes	,
T1898	4759-4761	CD	denotes	13
T1899	4761-4762	,	denotes	,
T1900	4762-4764	CD	denotes	14
T1901	4764-4765	,	denotes	,
T1895	4765-4767	CD	denotes	15
T1902	4767-4768	-RRB-	denotes	]
T1903	4768-4769	.	denotes	.
T1904	4769-5004	sentence	denotes	Both activity of Snail and down-regulation of E-cadherin play pivotal roles in epithelial to mesenchymal transitions (EMTs), typified by the transformation of polarized, adhering epithelial cells into motile mesenchymal cells [16,17].
T1905	4770-4774	CC	denotes	Both
T1906	4775-4783	NN	denotes	activity
T1908	4784-4786	IN	denotes	of
T1909	4787-4792	NN	denotes	Snail
T1910	4793-4796	CC	denotes	and
T1911	4797-4801	NN	denotes	down
T1913	4801-4802	HYPH	denotes	-
T1912	4802-4812	NN	denotes	regulation
T1914	4813-4815	IN	denotes	of
T1915	4816-4817	NN	denotes	E
T1917	4817-4818	HYPH	denotes	-
T1916	4818-4826	NN	denotes	cadherin
T1907	4827-4831	VBP	denotes	play
T1918	4832-4839	JJ	denotes	pivotal
T1919	4840-4845	NNS	denotes	roles
T1920	4846-4848	IN	denotes	in
T1921	4849-4859	JJ	denotes	epithelial
T1923	4860-4862	IN	denotes	to
T1924	4863-4874	JJ	denotes	mesenchymal
T1922	4875-4886	NNS	denotes	transitions
T1925	4887-4888	-LRB-	denotes	(
T1926	4888-4892	NNS	denotes	EMTs
T1927	4892-4893	-RRB-	denotes	)
T1928	4893-4895	,	denotes	,
T1929	4895-4903	VBN	denotes	typified
T1930	4904-4906	IN	denotes	by
T1931	4907-4910	DT	denotes	the
T1932	4911-4925	NN	denotes	transformation
T1933	4926-4928	IN	denotes	of
T1934	4929-4938	VBN	denotes	polarized
T1936	4938-4940	,	denotes	,
T1937	4940-4948	VBG	denotes	adhering
T1938	4949-4959	JJ	denotes	epithelial
T1935	4960-4965	NNS	denotes	cells
T1939	4966-4970	IN	denotes	into
T1940	4971-4977	JJ	denotes	motile
T1942	4978-4989	JJ	denotes	mesenchymal
T1941	4990-4995	NNS	denotes	cells
T1943	4996-4997	-LRB-	denotes	[
T1945	4997-4999	CD	denotes	16
T1946	4999-5000	,	denotes	,
T1944	5000-5002	CD	denotes	17
T1947	5002-5003	-RRB-	denotes	]
T1948	5003-5004	.	denotes	.
T1949	5004-5190	sentence	denotes	Bud formation differs from an EMT in that E-cadherin activity needs to be down-regulated but not prevented, so that adhesive junctions are remodeled rather than quantitatively impaired.
T1950	5005-5008	NN	denotes	Bud
T1951	5009-5018	NN	denotes	formation
T1952	5019-5026	VBZ	denotes	differs
T1953	5027-5031	IN	denotes	from
T1954	5032-5034	DT	denotes	an
T1955	5035-5038	NN	denotes	EMT
T1956	5039-5041	IN	denotes	in
T1958	5042-5046	DT	denotes	that
T1959	5047-5048	NN	denotes	E
T1961	5048-5049	HYPH	denotes	-
T1960	5049-5057	NN	denotes	cadherin
T1962	5058-5066	NN	denotes	activity
T1957	5067-5072	VBZ	denotes	needs
T1963	5073-5075	TO	denotes	to
T1965	5076-5078	VB	denotes	be
T1966	5079-5083	RB	denotes	down
T1967	5083-5084	HYPH	denotes	-
T1964	5084-5093	VBN	denotes	regulated
T1968	5094-5097	CC	denotes	but
T1969	5098-5101	RB	denotes	not
T1970	5102-5111	VBN	denotes	prevented
T1971	5111-5113	,	denotes	,
T1972	5113-5115	IN	denotes	so
T1974	5116-5120	IN	denotes	that
T1975	5121-5129	JJ	denotes	adhesive
T1976	5130-5139	NNS	denotes	junctions
T1977	5140-5143	VBP	denotes	are
T1973	5144-5153	VBN	denotes	remodeled
T1978	5154-5160	RB	denotes	rather
T1979	5161-5165	IN	denotes	than
T1980	5166-5180	RB	denotes	quantitatively
T1981	5181-5189	VBN	denotes	impaired
T1982	5189-5190	.	denotes	.
T1983	5190-5408	sentence	denotes	Supportive of an underlying ability to fine-tune cadherin expression at the transcriptional level, Snail seems to have an additive effect with LEF-1/β-catenin in negatively modulating E-cadherin promoter activity [4].
T1984	5191-5201	JJ	denotes	Supportive
T1986	5202-5204	IN	denotes	of
T1987	5205-5207	DT	denotes	an
T1989	5208-5218	JJ	denotes	underlying
T1988	5219-5226	NN	denotes	ability
T1990	5227-5229	TO	denotes	to
T1992	5230-5234	RB	denotes	fine
T1993	5234-5235	HYPH	denotes	-
T1991	5235-5239	VB	denotes	tune
T1994	5240-5248	NN	denotes	cadherin
T1995	5249-5259	NN	denotes	expression
T1996	5260-5262	IN	denotes	at
T1997	5263-5266	DT	denotes	the
T1999	5267-5282	JJ	denotes	transcriptional
T1998	5283-5288	NN	denotes	level
T2000	5288-5290	,	denotes	,
T2001	5290-5295	NN	denotes	Snail
T1985	5296-5301	VBZ	denotes	seems
T2002	5302-5304	TO	denotes	to
T2003	5305-5309	VB	denotes	have
T2004	5310-5312	DT	denotes	an
T2006	5313-5321	JJ	denotes	additive
T2005	5322-5328	NN	denotes	effect
T2007	5329-5333	IN	denotes	with
T2008	5334-5337	NN	denotes	LEF
T2009	5337-5338	HYPH	denotes	-
T2010	5338-5339	CD	denotes	1
T2011	5339-5340	HYPH	denotes	/
T2012	5340-5341	NN	denotes	β
T2014	5341-5342	HYPH	denotes	-
T2013	5342-5349	NN	denotes	catenin
T2015	5350-5352	IN	denotes	in
T2016	5353-5363	RB	denotes	negatively
T2017	5364-5374	VBG	denotes	modulating
T2018	5375-5376	NN	denotes	E
T2020	5376-5377	HYPH	denotes	-
T2019	5377-5385	NN	denotes	cadherin
T2021	5386-5394	NN	denotes	promoter
T2022	5395-5403	NN	denotes	activity
T2023	5404-5405	-LRB-	denotes	[
T2024	5405-5406	CD	denotes	4
T2025	5406-5407	-RRB-	denotes	]
T2026	5407-5408	.	denotes	.
T2027	5408-5593	sentence	denotes	In the present study, we discovered that Snail is expressed briefly at an early stage of hair bud formation, when E-cadherin down-regulation and activation of proliferation take place.
T2028	5409-5411	IN	denotes	In
T2030	5412-5415	DT	denotes	the
T2032	5416-5423	JJ	denotes	present
T2031	5424-5429	NN	denotes	study
T2033	5429-5431	,	denotes	,
T2034	5431-5433	PRP	denotes	we
T2029	5434-5444	VBD	denotes	discovered
T2035	5445-5449	IN	denotes	that
T2037	5450-5455	NN	denotes	Snail
T2038	5456-5458	VBZ	denotes	is
T2036	5459-5468	VBN	denotes	expressed
T2039	5469-5476	RB	denotes	briefly
T2040	5477-5479	IN	denotes	at
T2041	5480-5482	DT	denotes	an
T2043	5483-5488	JJ	denotes	early
T2042	5489-5494	NN	denotes	stage
T2044	5495-5497	IN	denotes	of
T2045	5498-5502	NN	denotes	hair
T2046	5503-5506	NN	denotes	bud
T2047	5507-5516	NN	denotes	formation
T2048	5516-5518	,	denotes	,
T2049	5518-5522	WRB	denotes	when
T2051	5523-5524	NN	denotes	E
T2053	5524-5525	HYPH	denotes	-
T2052	5525-5533	NN	denotes	cadherin
T2054	5534-5538	JJ	denotes	down
T2056	5538-5539	HYPH	denotes	-
T2055	5539-5549	NN	denotes	regulation
T2057	5550-5553	CC	denotes	and
T2058	5554-5564	NN	denotes	activation
T2059	5565-5567	IN	denotes	of
T2060	5568-5581	NN	denotes	proliferation
T2050	5582-5586	VBP	denotes	take
T2061	5587-5592	NN	denotes	place
T2062	5592-5593	.	denotes	.
T2063	5593-5696	sentence	denotes	Thereafter, Snail disappears and remains absent during subsequent follicle down-growth and maturation.
T2064	5594-5604	RB	denotes	Thereafter
T2066	5604-5606	,	denotes	,
T2067	5606-5611	NN	denotes	Snail
T2065	5612-5622	VBZ	denotes	disappears
T2068	5623-5626	CC	denotes	and
T2069	5627-5634	VBZ	denotes	remains
T2070	5635-5641	JJ	denotes	absent
T2071	5642-5648	IN	denotes	during
T2072	5649-5659	JJ	denotes	subsequent
T2074	5660-5668	NN	denotes	follicle
T2075	5669-5673	JJ	denotes	down
T2076	5673-5674	HYPH	denotes	-
T2073	5674-5680	NN	denotes	growth
T2077	5681-5684	CC	denotes	and
T2078	5685-5695	NN	denotes	maturation
T2079	5695-5696	.	denotes	.
T2080	5696-5956	sentence	denotes	This exquisite pattern appears to be functionally relevant since altering it in vivo correspondingly affects features associated with hair bud formation, including down-regulation of E-cadherin, increased proliferation, and repressed terminal differentiation.
T2081	5697-5701	DT	denotes	This
T2083	5702-5711	JJ	denotes	exquisite
T2082	5712-5719	NN	denotes	pattern
T2084	5720-5727	VBZ	denotes	appears
T2085	5728-5730	TO	denotes	to
T2086	5731-5733	VB	denotes	be
T2087	5734-5746	RB	denotes	functionally
T2088	5747-5755	JJ	denotes	relevant
T2089	5756-5761	IN	denotes	since
T2091	5762-5770	VBG	denotes	altering
T2092	5771-5773	PRP	denotes	it
T2093	5774-5776	FW	denotes	in
T2094	5777-5781	FW	denotes	vivo
T2095	5782-5797	RB	denotes	correspondingly
T2090	5798-5805	VBZ	denotes	affects
T2096	5806-5814	NNS	denotes	features
T2097	5815-5825	VBN	denotes	associated
T2098	5826-5830	IN	denotes	with
T2099	5831-5835	NN	denotes	hair
T2100	5836-5839	NN	denotes	bud
T2101	5840-5849	NN	denotes	formation
T2102	5849-5851	,	denotes	,
T2103	5851-5860	VBG	denotes	including
T2104	5861-5865	NN	denotes	down
T2106	5865-5866	HYPH	denotes	-
T2105	5866-5876	NN	denotes	regulation
T2107	5877-5879	IN	denotes	of
T2108	5880-5881	NN	denotes	E
T2110	5881-5882	HYPH	denotes	-
T2109	5882-5890	NN	denotes	cadherin
T2111	5890-5892	,	denotes	,
T2112	5892-5901	VBN	denotes	increased
T2113	5902-5915	NN	denotes	proliferation
T2114	5915-5917	,	denotes	,
T2115	5917-5920	CC	denotes	and
T2116	5921-5930	VBN	denotes	repressed
T2118	5931-5939	JJ	denotes	terminal
T2117	5940-5955	NN	denotes	differentiation
T2119	5955-5956	.	denotes	.
T2120	5956-6202	sentence	denotes	Although the temporal spike of Snail in the hair bud is reflected at the mRNA level and seems to follow Wnt signaling and BMP inhibition, LEF-1/β-catenin activation does not appear to induce Snail gene expression in embryonic skin keratinocytes.
T2121	5957-5965	IN	denotes	Although
T2123	5966-5969	DT	denotes	the
T2125	5970-5978	JJ	denotes	temporal
T2124	5979-5984	NN	denotes	spike
T2126	5985-5987	IN	denotes	of
T2127	5988-5993	NN	denotes	Snail
T2128	5994-5996	IN	denotes	in
T2129	5997-6000	DT	denotes	the
T2131	6001-6005	NN	denotes	hair
T2130	6006-6009	NN	denotes	bud
T2132	6010-6012	VBZ	denotes	is
T2122	6013-6022	VBN	denotes	reflected
T2134	6023-6025	IN	denotes	at
T2135	6026-6029	DT	denotes	the
T2137	6030-6034	NN	denotes	mRNA
T2136	6035-6040	NN	denotes	level
T2138	6041-6044	CC	denotes	and
T2139	6045-6050	VBZ	denotes	seems
T2140	6051-6053	TO	denotes	to
T2141	6054-6060	VB	denotes	follow
T2142	6061-6064	NN	denotes	Wnt
T2143	6065-6074	NN	denotes	signaling
T2144	6075-6078	CC	denotes	and
T2145	6079-6082	NN	denotes	BMP
T2146	6083-6093	NN	denotes	inhibition
T2147	6093-6095	,	denotes	,
T2148	6095-6098	NN	denotes	LEF
T2150	6098-6099	HYPH	denotes	-
T2151	6099-6100	CD	denotes	1
T2152	6100-6101	HYPH	denotes	/
T2153	6101-6102	NN	denotes	β
T2155	6102-6103	HYPH	denotes	-
T2154	6103-6110	NN	denotes	catenin
T2149	6111-6121	NN	denotes	activation
T2156	6122-6126	VBZ	denotes	does
T2157	6127-6130	RB	denotes	not
T2133	6131-6137	VB	denotes	appear
T2158	6138-6140	TO	denotes	to
T2159	6141-6147	VB	denotes	induce
T2160	6148-6153	NN	denotes	Snail
T2162	6154-6158	NN	denotes	gene
T2161	6159-6169	NN	denotes	expression
T2163	6170-6172	IN	denotes	in
T2164	6173-6182	JJ	denotes	embryonic
T2166	6183-6187	NN	denotes	skin
T2165	6188-6201	NNS	denotes	keratinocytes
T2167	6201-6202	.	denotes	.
T2168	6202-6461	sentence	denotes	In contrast, we provide in vitro, transgenic (Tg), and gene targeting evidence to show that TGF-β2 and small phenotype– and mothers against decapentaplegic–related protein 2 (SMAD2) signaling are upstream inducers of Snail gene expression in skin epithelium.
T2169	6203-6205	IN	denotes	In
T2171	6206-6214	NN	denotes	contrast
T2172	6214-6216	,	denotes	,
T2173	6216-6218	PRP	denotes	we
T2170	6219-6226	VBP	denotes	provide
T2174	6227-6229	FW	denotes	in
T2175	6230-6235	FW	denotes	vitro
T2177	6235-6237	,	denotes	,
T2178	6237-6247	JJ	denotes	transgenic
T2179	6248-6249	-LRB-	denotes	(
T2180	6249-6251	JJ	denotes	Tg
T2181	6251-6252	-RRB-	denotes	)
T2182	6252-6254	,	denotes	,
T2183	6254-6257	CC	denotes	and
T2184	6258-6262	NN	denotes	gene
T2185	6263-6272	VBG	denotes	targeting
T2176	6273-6281	NN	denotes	evidence
T2186	6282-6284	TO	denotes	to
T2187	6285-6289	VB	denotes	show
T2188	6290-6294	IN	denotes	that
T2190	6295-6298	NN	denotes	TGF
T2192	6298-6299	HYPH	denotes	-
T2191	6299-6301	NN	denotes	β2
T2194	6302-6305	CC	denotes	and
T2195	6306-6311	JJ	denotes	small
T2196	6312-6321	NN	denotes	phenotype
T2198	6321-6322	-LRB-	denotes	–
T2199	6323-6326	CC	denotes	and
T2200	6327-6334	NNS	denotes	mothers
T2201	6335-6342	IN	denotes	against
T2202	6343-6358	NN	denotes	decapentaplegic
T2203	6358-6359	-RRB-	denotes	–
T2197	6359-6366	VBN	denotes	related
T2204	6367-6374	NN	denotes	protein
T2205	6375-6376	CD	denotes	2
T2206	6377-6378	-LRB-	denotes	(
T2207	6378-6383	NN	denotes	SMAD2
T2208	6383-6384	-RRB-	denotes	)
T2193	6385-6394	NN	denotes	signaling
T2189	6395-6398	VBP	denotes	are
T2209	6399-6407	JJ	denotes	upstream
T2210	6408-6416	NNS	denotes	inducers
T2211	6417-6419	IN	denotes	of
T2212	6420-6425	NN	denotes	Snail
T2214	6426-6430	NN	denotes	gene
T2213	6431-6441	NN	denotes	expression
T2215	6442-6444	IN	denotes	in
T2216	6445-6449	NN	denotes	skin
T2217	6450-6460	NN	denotes	epithelium
T2218	6460-6461	.	denotes	.
T2219	6461-6592	sentence	denotes	In the absence of TGF-β2 signaling and Snail gene expression, hair placodes can form, but further follicle down-growth is blocked.
T2220	6462-6464	IN	denotes	In
T2222	6465-6468	DT	denotes	the
T2223	6469-6476	NN	denotes	absence
T2224	6477-6479	IN	denotes	of
T2225	6480-6483	NN	denotes	TGF
T2227	6483-6484	HYPH	denotes	-
T2226	6484-6486	NN	denotes	β2
T2228	6487-6496	NN	denotes	signaling
T2229	6497-6500	CC	denotes	and
T2230	6501-6506	NN	denotes	Snail
T2232	6507-6511	NN	denotes	gene
T2231	6512-6522	NN	denotes	expression
T2233	6522-6524	,	denotes	,
T2234	6524-6528	NN	denotes	hair
T2235	6529-6537	NNS	denotes	placodes
T2236	6538-6541	MD	denotes	can
T2221	6542-6546	VB	denotes	form
T2237	6546-6548	,	denotes	,
T2238	6548-6551	CC	denotes	but
T2239	6552-6559	JJ	denotes	further
T2241	6560-6568	NN	denotes	follicle
T2242	6569-6573	JJ	denotes	down
T2243	6573-6574	HYPH	denotes	-
T2240	6574-6580	NN	denotes	growth
T2245	6581-6583	VBZ	denotes	is
T2244	6584-6591	VBN	denotes	blocked
T2246	6591-6592	.	denotes	.
T2247	6592-6770	sentence	denotes	Our studies point to the view that Snail likely functions downstream of cell fate specification, at a stage where the bud begins to exhibit enhanced proliferation and migration.
T2248	6593-6596	PRP$	denotes	Our
T2249	6597-6604	NNS	denotes	studies
T2250	6605-6610	VBP	denotes	point
T2251	6611-6613	IN	denotes	to
T2252	6614-6617	DT	denotes	the
T2253	6618-6622	NN	denotes	view
T2254	6623-6627	IN	denotes	that
T2256	6628-6633	NN	denotes	Snail
T2257	6634-6640	RB	denotes	likely
T2255	6641-6650	VBZ	denotes	functions
T2258	6651-6661	RB	denotes	downstream
T2259	6662-6664	IN	denotes	of
T2260	6665-6669	NN	denotes	cell
T2261	6670-6674	NN	denotes	fate
T2262	6675-6688	NN	denotes	specification
T2263	6688-6690	,	denotes	,
T2264	6690-6692	IN	denotes	at
T2265	6693-6694	DT	denotes	a
T2266	6695-6700	NN	denotes	stage
T2267	6701-6706	WRB	denotes	where
T2269	6707-6710	DT	denotes	the
T2270	6711-6714	NN	denotes	bud
T2268	6715-6721	VBZ	denotes	begins
T2271	6722-6724	TO	denotes	to
T2272	6725-6732	VB	denotes	exhibit
T2273	6733-6741	VBN	denotes	enhanced
T2274	6742-6755	NN	denotes	proliferation
T2275	6756-6759	CC	denotes	and
T2276	6760-6769	NN	denotes	migration
T2277	6769-6770	.	denotes	.
T2702	6781-6786	NN	denotes	Snail
T2703	6787-6791	NN	denotes	mRNA
T2705	6792-6795	CC	denotes	and
T2706	6796-6803	NN	denotes	Protein
T2707	6804-6807	VBP	denotes	Are
T2704	6808-6817	VBN	denotes	Expressed
T2708	6818-6829	RB	denotes	Transiently
T2709	6830-6832	IN	denotes	at
T2710	6833-6836	DT	denotes	the
T2712	6837-6841	NN	denotes	Hair
T2713	6842-6845	NN	denotes	Bud
T2711	6846-6851	NN	denotes	Stage
T2714	6852-6854	IN	denotes	of
T2715	6855-6863	NN	denotes	Follicle
T2716	6864-6877	NN	denotes	Morphogenesis
T2717	6877-7094	sentence	denotes	Although Snail family members are most frequently associated with EMTs, they also participate in many malignant processes involving a down-regulation but not a quantitative abrogation of intercellular junctions [18].
T2718	6878-6886	IN	denotes	Although
T2720	6887-6892	NN	denotes	Snail
T2722	6893-6899	NN	denotes	family
T2721	6900-6907	NNS	denotes	members
T2723	6908-6911	VBP	denotes	are
T2724	6912-6916	RBS	denotes	most
T2725	6917-6927	RB	denotes	frequently
T2719	6928-6938	VBN	denotes	associated
T2727	6939-6943	IN	denotes	with
T2728	6944-6948	NNS	denotes	EMTs
T2729	6948-6950	,	denotes	,
T2730	6950-6954	PRP	denotes	they
T2731	6955-6959	RB	denotes	also
T2726	6960-6971	VBP	denotes	participate
T2732	6972-6974	IN	denotes	in
T2733	6975-6979	JJ	denotes	many
T2735	6980-6989	JJ	denotes	malignant
T2734	6990-6999	NNS	denotes	processes
T2736	7000-7009	VBG	denotes	involving
T2737	7010-7011	DT	denotes	a
T2739	7012-7016	JJ	denotes	down
T2740	7016-7017	HYPH	denotes	-
T2738	7017-7027	NN	denotes	regulation
T2741	7028-7031	CC	denotes	but
T2742	7032-7035	RB	denotes	not
T2743	7036-7037	DT	denotes	a
T2745	7038-7050	JJ	denotes	quantitative
T2744	7051-7061	NN	denotes	abrogation
T2746	7062-7064	IN	denotes	of
T2747	7065-7078	JJ	denotes	intercellular
T2748	7079-7088	NNS	denotes	junctions
T2749	7089-7090	-LRB-	denotes	[
T2750	7090-7092	CD	denotes	18
T2751	7092-7093	-RRB-	denotes	]
T2752	7093-7094	.	denotes	.
T2753	7094-7279	sentence	denotes	The range of developmental processes in which Snail family members have been implicated thus includes the type of epithelial remodeling that is observed in hair follicle bud formation.
T2754	7095-7098	DT	denotes	The
T2755	7099-7104	NN	denotes	range
T2757	7105-7107	IN	denotes	of
T2758	7108-7121	JJ	denotes	developmental
T2759	7122-7131	NNS	denotes	processes
T2760	7132-7134	IN	denotes	in
T2762	7135-7140	WDT	denotes	which
T2763	7141-7146	NN	denotes	Snail
T2765	7147-7153	NN	denotes	family
T2764	7154-7161	NNS	denotes	members
T2766	7162-7166	VBP	denotes	have
T2767	7167-7171	VBN	denotes	been
T2761	7172-7182	VBN	denotes	implicated
T2768	7183-7187	RB	denotes	thus
T2756	7188-7196	VBZ	denotes	includes
T2769	7197-7200	DT	denotes	the
T2770	7201-7205	NN	denotes	type
T2771	7206-7208	IN	denotes	of
T2772	7209-7219	JJ	denotes	epithelial
T2773	7220-7230	NN	denotes	remodeling
T2774	7231-7235	WDT	denotes	that
T2776	7236-7238	VBZ	denotes	is
T2775	7239-7247	VBN	denotes	observed
T2777	7248-7250	IN	denotes	in
T2778	7251-7255	NN	denotes	hair
T2779	7256-7264	NN	denotes	follicle
T2780	7265-7268	NN	denotes	bud
T2781	7269-7278	NN	denotes	formation
T2782	7278-7279	.	denotes	.
T2783	7279-7633	sentence	denotes	Given our prior observation that exogenously added Snail can participate with LEF-1/β-catenin in down-regulating E-cadherin expression in keratinocytes [4], coupled with the established requirement for LEF-1/β-catenin in hair follicle morphogenesis [4,19], we turned to addressing whether Snail/Slug family members might also participate in the process.
T2784	7280-7285	VBN	denotes	Given
T2786	7286-7289	PRP$	denotes	our
T2788	7290-7295	JJ	denotes	prior
T2787	7296-7307	NN	denotes	observation
T2789	7308-7312	IN	denotes	that
T2791	7313-7324	RB	denotes	exogenously
T2792	7325-7330	VBN	denotes	added
T2793	7331-7336	NN	denotes	Snail
T2794	7337-7340	MD	denotes	can
T2790	7341-7352	VB	denotes	participate
T2795	7353-7357	IN	denotes	with
T2796	7358-7361	NN	denotes	LEF
T2797	7361-7362	HYPH	denotes	-
T2798	7362-7363	CD	denotes	1
T2799	7363-7364	HYPH	denotes	/
T2800	7364-7365	NN	denotes	β
T2802	7365-7366	HYPH	denotes	-
T2801	7366-7373	NN	denotes	catenin
T2803	7374-7376	IN	denotes	in
T2804	7377-7381	RB	denotes	down
T2806	7381-7382	HYPH	denotes	-
T2805	7382-7392	VBG	denotes	regulating
T2807	7393-7394	NN	denotes	E
T2809	7394-7395	HYPH	denotes	-
T2808	7395-7403	NN	denotes	cadherin
T2810	7404-7414	NN	denotes	expression
T2811	7415-7417	IN	denotes	in
T2812	7418-7431	NNS	denotes	keratinocytes
T2813	7432-7433	-LRB-	denotes	[
T2814	7433-7434	CD	denotes	4
T2815	7434-7435	-RRB-	denotes	]
T2816	7435-7437	,	denotes	,
T2817	7437-7444	VBN	denotes	coupled
T2818	7445-7449	IN	denotes	with
T2819	7450-7453	DT	denotes	the
T2821	7454-7465	JJ	denotes	established
T2820	7466-7477	NN	denotes	requirement
T2822	7478-7481	IN	denotes	for
T2823	7482-7485	NN	denotes	LEF
T2824	7485-7486	HYPH	denotes	-
T2825	7486-7487	CD	denotes	1
T2826	7487-7488	HYPH	denotes	/
T2827	7488-7489	NN	denotes	β
T2829	7489-7490	HYPH	denotes	-
T2828	7490-7497	NN	denotes	catenin
T2830	7498-7500	IN	denotes	in
T2831	7501-7505	NN	denotes	hair
T2832	7506-7514	NN	denotes	follicle
T2833	7515-7528	NN	denotes	morphogenesis
T2834	7529-7530	-LRB-	denotes	[
T2836	7530-7531	CD	denotes	4
T2837	7531-7532	,	denotes	,
T2835	7532-7534	CD	denotes	19
T2838	7534-7535	-RRB-	denotes	]
T2839	7535-7537	,	denotes	,
T2840	7537-7539	PRP	denotes	we
T2785	7540-7546	VBD	denotes	turned
T2841	7547-7549	IN	denotes	to
T2842	7550-7560	VBG	denotes	addressing
T2843	7561-7568	IN	denotes	whether
T2845	7569-7574	NN	denotes	Snail
T2847	7574-7575	HYPH	denotes	/
T2846	7575-7579	NN	denotes	Slug
T2849	7580-7586	NN	denotes	family
T2848	7587-7594	NNS	denotes	members
T2850	7595-7600	MD	denotes	might
T2851	7601-7605	RB	denotes	also
T2844	7606-7617	VB	denotes	participate
T2852	7618-7620	IN	denotes	in
T2853	7621-7624	DT	denotes	the
T2854	7625-7632	NN	denotes	process
T2855	7632-7633	.	denotes	.
T2856	7633-7788	sentence	denotes	PCR analyses identified transient Snail mRNA expression during a period of skin embryogenesis when waves of hair follicles are forming (unpublished data).
T2857	7634-7637	NN	denotes	PCR
T2858	7638-7646	NNS	denotes	analyses
T2859	7647-7657	VBD	denotes	identified
T2860	7658-7667	JJ	denotes	transient
T2862	7668-7673	NN	denotes	Snail
T2863	7674-7678	NN	denotes	mRNA
T2861	7679-7689	NN	denotes	expression
T2864	7690-7696	IN	denotes	during
T2865	7697-7698	DT	denotes	a
T2866	7699-7705	NN	denotes	period
T2867	7706-7708	IN	denotes	of
T2868	7709-7713	NN	denotes	skin
T2869	7714-7727	NN	denotes	embryogenesis
T2870	7728-7732	WRB	denotes	when
T2872	7733-7738	NNS	denotes	waves
T2873	7739-7741	IN	denotes	of
T2874	7742-7746	NN	denotes	hair
T2875	7747-7756	NNS	denotes	follicles
T2876	7757-7760	VBP	denotes	are
T2871	7761-7768	VBG	denotes	forming
T2877	7769-7770	-LRB-	denotes	(
T2879	7770-7781	JJ	denotes	unpublished
T2878	7782-7786	NNS	denotes	data
T2880	7786-7787	-RRB-	denotes	)
T2881	7787-7788	.	denotes	.
T2882	7788-7790	TO	denotes	To
T2884	7788-7970	sentence	denotes	To pinpoint specifically where Snail mRNA is expressed in the developing skin, we conducted in situ hybridization using a cRNA probe unique to the Snail 3′ untranslated region (UTR).
T2883	7791-7799	VB	denotes	pinpoint
T2886	7800-7812	RB	denotes	specifically
T2887	7813-7818	WRB	denotes	where
T2889	7819-7824	NN	denotes	Snail
T2890	7825-7829	NN	denotes	mRNA
T2891	7830-7832	VBZ	denotes	is
T2888	7833-7842	VBN	denotes	expressed
T2892	7843-7845	IN	denotes	in
T2893	7846-7849	DT	denotes	the
T2895	7850-7860	VBG	denotes	developing
T2894	7861-7865	NN	denotes	skin
T2896	7865-7867	,	denotes	,
T2897	7867-7869	PRP	denotes	we
T2885	7870-7879	VBD	denotes	conducted
T2898	7880-7882	FW	denotes	in
T2899	7883-7887	FW	denotes	situ
T2900	7888-7901	NN	denotes	hybridization
T2901	7902-7907	VBG	denotes	using
T2902	7908-7909	DT	denotes	a
T2904	7910-7914	NN	denotes	cRNA
T2903	7915-7920	NN	denotes	probe
T2905	7921-7927	JJ	denotes	unique
T2906	7928-7930	IN	denotes	to
T2907	7931-7934	DT	denotes	the
T2909	7935-7940	NNP	denotes	Snail
T2910	7941-7942	CD	denotes	3
T2911	7942-7943	SYM	denotes	′
T2912	7944-7956	JJ	denotes	untranslated
T2908	7957-7963	NN	denotes	region
T2913	7964-7965	-LRB-	denotes	(
T2914	7965-7968	NN	denotes	UTR
T2915	7968-7969	-RRB-	denotes	)
T2916	7969-7970	.	denotes	.
T2917	7970-8159	sentence	denotes	Embryonic day 17.5 (E17.5) was chosen, since the multiple waves of follicle morphogenesis occurring at this time enabled us to evaluate Snail expression at different stages of the process.
T2918	7971-7980	JJ	denotes	Embryonic
T2919	7981-7984	NN	denotes	day
T2921	7985-7989	CD	denotes	17.5
T2922	7990-7991	-LRB-	denotes	(
T2923	7991-7996	NN	denotes	E17.5
T2924	7996-7997	-RRB-	denotes	)
T2925	7998-8001	VBD	denotes	was
T2920	8002-8008	VBN	denotes	chosen
T2926	8008-8010	,	denotes	,
T2927	8010-8015	IN	denotes	since
T2929	8016-8019	DT	denotes	the
T2931	8020-8028	JJ	denotes	multiple
T2930	8029-8034	NNS	denotes	waves
T2932	8035-8037	IN	denotes	of
T2933	8038-8046	NN	denotes	follicle
T2934	8047-8060	NN	denotes	morphogenesis
T2935	8061-8070	VBG	denotes	occurring
T2936	8071-8073	IN	denotes	at
T2937	8074-8078	DT	denotes	this
T2938	8079-8083	NN	denotes	time
T2928	8084-8091	VBD	denotes	enabled
T2939	8092-8094	PRP	denotes	us
T2940	8095-8097	TO	denotes	to
T2941	8098-8106	VB	denotes	evaluate
T2942	8107-8112	NN	denotes	Snail
T2943	8113-8123	NN	denotes	expression
T2944	8124-8126	IN	denotes	at
T2945	8127-8136	JJ	denotes	different
T2946	8137-8143	NNS	denotes	stages
T2947	8144-8146	IN	denotes	of
T2948	8147-8150	DT	denotes	the
T2949	8151-8158	NN	denotes	process
T2950	8158-8159	.	denotes	.
T2951	8159-8262	sentence	denotes	As shown in Figure 1A, specific hybridization was detected within the epithelium of nascent hair buds.
T2952	8160-8162	IN	denotes	As
T2953	8163-8168	VBN	denotes	shown
T2955	8169-8171	IN	denotes	in
T2956	8172-8178	NN	denotes	Figure
T2957	8179-8181	NN	denotes	1A
T2958	8181-8183	,	denotes	,
T2959	8183-8191	JJ	denotes	specific
T2960	8192-8205	NN	denotes	hybridization
T2961	8206-8209	VBD	denotes	was
T2954	8210-8218	VBN	denotes	detected
T2962	8219-8225	IN	denotes	within
T2963	8226-8229	DT	denotes	the
T2964	8230-8240	NN	denotes	epithelium
T2965	8241-8243	IN	denotes	of
T2966	8244-8251	JJ	denotes	nascent
T2968	8252-8256	NN	denotes	hair
T2967	8257-8261	NNS	denotes	buds
T2969	8261-8262	.	denotes	.
T2970	8262-8416	sentence	denotes	By contrast, as follicles progressed further through their development (e.g., germ and peg stages), they exhibited no signs of hybridization (Figure 1A).
T2971	8263-8265	IN	denotes	By
T2973	8266-8274	NN	denotes	contrast
T2974	8274-8276	,	denotes	,
T2975	8276-8278	IN	denotes	as
T2977	8279-8288	NNS	denotes	follicles
T2976	8289-8299	VBD	denotes	progressed
T2978	8300-8307	RBR	denotes	further
T2979	8308-8315	IN	denotes	through
T2980	8316-8321	PRP$	denotes	their
T2981	8322-8333	NN	denotes	development
T2982	8334-8335	-LRB-	denotes	(
T2984	8335-8339	FW	denotes	e.g.
T2985	8339-8341	,	denotes	,
T2986	8341-8345	NN	denotes	germ
T2987	8346-8349	CC	denotes	and
T2988	8350-8353	NN	denotes	peg
T2983	8354-8360	NNS	denotes	stages
T2989	8360-8361	-RRB-	denotes	)
T2990	8361-8363	,	denotes	,
T2991	8363-8367	PRP	denotes	they
T2972	8368-8377	VBD	denotes	exhibited
T2992	8378-8380	DT	denotes	no
T2993	8381-8386	NNS	denotes	signs
T2994	8387-8389	IN	denotes	of
T2995	8390-8403	NN	denotes	hybridization
T2996	8404-8405	-LRB-	denotes	(
T2998	8405-8411	NN	denotes	Figure
T2997	8412-8414	NN	denotes	1A
T2999	8414-8415	-RRB-	denotes	)
T3000	8415-8416	.	denotes	.
T3001	8416-8623	sentence	denotes	The transient nature of Snail mRNA expression during follicle development was most apparent in hybridized skin sections containing follicles from two different waves of morphogenesis (as shown in Figure 1).
T3002	8417-8420	DT	denotes	The
T3004	8421-8430	JJ	denotes	transient
T3003	8431-8437	NN	denotes	nature
T3006	8438-8440	IN	denotes	of
T3007	8441-8446	NN	denotes	Snail
T3009	8447-8451	NN	denotes	mRNA
T3008	8452-8462	NN	denotes	expression
T3010	8463-8469	IN	denotes	during
T3011	8470-8478	NN	denotes	follicle
T3012	8479-8490	NN	denotes	development
T3005	8491-8494	VBD	denotes	was
T3013	8495-8499	RBS	denotes	most
T3014	8500-8508	JJ	denotes	apparent
T3015	8509-8511	IN	denotes	in
T3016	8512-8522	VBN	denotes	hybridized
T3018	8523-8527	NN	denotes	skin
T3017	8528-8536	NNS	denotes	sections
T3019	8537-8547	VBG	denotes	containing
T3020	8548-8557	NNS	denotes	follicles
T3021	8558-8562	IN	denotes	from
T3022	8563-8566	CD	denotes	two
T3024	8567-8576	JJ	denotes	different
T3023	8577-8582	NNS	denotes	waves
T3025	8583-8585	IN	denotes	of
T3026	8586-8599	NN	denotes	morphogenesis
T3027	8600-8601	-LRB-	denotes	(
T3029	8601-8603	IN	denotes	as
T3028	8604-8609	VBN	denotes	shown
T3030	8610-8612	IN	denotes	in
T3031	8613-8619	NN	denotes	Figure
T3032	8620-8621	CD	denotes	1
T3033	8621-8622	-RRB-	denotes	)
T3034	8622-8623	.	denotes	.
T3035	8623-8735	sentence	denotes	Hybridizing hair buds from a later wave appeared juxtaposed with nonhybridizing follicles from an earlier wave.
T3036	8624-8635	VBG	denotes	Hybridizing
T3038	8636-8640	NN	denotes	hair
T3037	8641-8645	NNS	denotes	buds
T3040	8646-8650	IN	denotes	from
T3041	8651-8652	DT	denotes	a
T3043	8653-8658	JJ	denotes	later
T3042	8659-8663	NN	denotes	wave
T3039	8664-8672	VBD	denotes	appeared
T3044	8673-8683	JJ	denotes	juxtaposed
T3045	8684-8688	IN	denotes	with
T3046	8689-8703	JJ	denotes	nonhybridizing
T3047	8704-8713	NNS	denotes	follicles
T3048	8714-8718	IN	denotes	from
T3049	8719-8721	DT	denotes	an
T3051	8722-8729	JJR	denotes	earlier
T3050	8730-8734	NN	denotes	wave
T3052	8734-8735	.	denotes	.
T3053	8735-11356	sentence	denotes	Figure 1 Snail Is Expressed Exclusively in the Hair Bud during Morphogenesis Embryos were either frozen in OCT embedding compound (A, F, and H) or embedded in paraffin (C, D, E, and G), and then sectioned (8 μm). (A) In situ hybridizations with Snail sense or antisense cRNA probes. Black dotted lines demarcate the basement membrane that separates the epidermis (epi) from dermis (der). Arrows point to Snail RNA expression, restricted to the hair bud stage of follicle morphogenesis. It was not seen in later hair germ or peg stages. (B) Expression of Snail protein coincides with hair development. Protein extracts were prepared from keratinocytes transfected with empty expression vector (K14), containing the K14 promoter or with the vector driving HA-tagged Snail (K14-Snail); or from whole skin from E13.5 to P5 animals, including newborn (nb). Equal amounts of proteins were then resolved by SDS-PAGE through 12% gels and subjected to Western blotting using either an affinity-purified Snail polyclonal antiserum, which we generated, or anti-tubulin (loading control). (C–E) Immunohistochemistry shows expression of Snail protein in the nuclei of cells within the hair and skin. (C) E13.5 skin with a single layered epidermis (epi) shows no Snail expression. (D) The first morphological sign that cells have adopted a hair follicle fate is a cluster of cells called a placode in E16.5 skin. Snail is not expressed at this stage of development. (E) Snail is expressed in the hair bud of E17.5 skin but not in later stages of development such as the germ or peg. (F) Immunofluorescence with anti-Ki67 (green) identifies the proliferating cells of the skin, restricted to the basal layer of the epidermis and developing hair follicles. Anti-β4 int labeling reveals the presence of the hemidesmosomal integrin β4, restricted to the base of cells adhering to the underlying basement membrane. The white dotted line marks the outermost surface of the skin. (G) Immunohistochemistry with pMAPK marks a subset of proliferating cells within the epidermis and hair bud. Anti-pMAPK labeling was consistently robust within the hair bud. (H) Immunofluorescence with anti-laminin 5 (lam5), which demarcates the basement membrane, and anti-E-cadherin (E-cad), a component of AJs. At the leading edge of the growing bud, cell-cell borders show markedly diminished anti-E-cadherin labeling (arrowheads). To determine whether this transient nature of Snail mRNA expression is reflected at the protein level, we generated an antibody against the N-terminal sequence that resides upstream of the more conserved zinc finger domains.
T3054	11132-11134	TO	denotes	To
T3055	11135-11144	VB	denotes	determine
T3057	11145-11152	IN	denotes	whether
T3059	11153-11157	DT	denotes	this
T3061	11158-11167	JJ	denotes	transient
T3060	11168-11174	NN	denotes	nature
T3062	11175-11177	IN	denotes	of
T3063	11178-11183	NN	denotes	Snail
T3065	11184-11188	NN	denotes	mRNA
T3064	11189-11199	NN	denotes	expression
T3066	11200-11202	VBZ	denotes	is
T3058	11203-11212	VBN	denotes	reflected
T3067	11213-11215	IN	denotes	at
T3068	11216-11219	DT	denotes	the
T3070	11220-11227	NN	denotes	protein
T3069	11228-11233	NN	denotes	level
T3071	11233-11235	,	denotes	,
T3072	11235-11237	PRP	denotes	we
T3056	11238-11247	VBD	denotes	generated
T3073	11248-11250	DT	denotes	an
T3074	11251-11259	NN	denotes	antibody
T3075	11260-11267	IN	denotes	against
T3076	11268-11271	DT	denotes	the
T3078	11272-11273	NN	denotes	N
T3080	11273-11274	HYPH	denotes	-
T3079	11274-11282	JJ	denotes	terminal
T3077	11283-11291	NN	denotes	sequence
T3081	11292-11296	WDT	denotes	that
T3082	11297-11304	VBZ	denotes	resides
T3083	11305-11313	RB	denotes	upstream
T3084	11314-11316	IN	denotes	of
T3085	11317-11320	DT	denotes	the
T3087	11321-11325	RBR	denotes	more
T3088	11326-11335	VBN	denotes	conserved
T3089	11336-11340	NN	denotes	zinc
T3090	11341-11347	NN	denotes	finger
T3086	11348-11355	NNS	denotes	domains
T3091	11355-11356	.	denotes	.
T3092	11356-11611	sentence	denotes	As judged by Western blot analysis, the antibody did not detect endogenous proteins from cultured keratinocytes, but it did yield a band of the expected size from keratinocytes transiently expressing a hemagglutinin (HA)-tagged Snail protein (Figure 1B).
T3093	11357-11359	IN	denotes	As
T3094	11360-11366	VBN	denotes	judged
T3096	11367-11369	IN	denotes	by
T3097	11370-11377	NNP	denotes	Western
T3098	11378-11382	NN	denotes	blot
T3099	11383-11391	NN	denotes	analysis
T3100	11391-11393	,	denotes	,
T3101	11393-11396	DT	denotes	the
T3102	11397-11405	NN	denotes	antibody
T3103	11406-11409	VBD	denotes	did
T3104	11410-11413	RB	denotes	not
T3095	11414-11420	VB	denotes	detect
T3105	11421-11431	JJ	denotes	endogenous
T3106	11432-11440	NN	denotes	proteins
T3107	11441-11445	IN	denotes	from
T3108	11446-11454	VBN	denotes	cultured
T3109	11455-11468	NNS	denotes	keratinocytes
T3110	11468-11470	,	denotes	,
T3111	11470-11473	CC	denotes	but
T3112	11474-11476	PRP	denotes	it
T3114	11477-11480	VBD	denotes	did
T3113	11481-11486	VB	denotes	yield
T3115	11487-11488	DT	denotes	a
T3116	11489-11493	NN	denotes	band
T3117	11494-11496	IN	denotes	of
T3118	11497-11500	DT	denotes	the
T3120	11501-11509	VBN	denotes	expected
T3119	11510-11514	NN	denotes	size
T3121	11515-11519	IN	denotes	from
T3122	11520-11533	NNS	denotes	keratinocytes
T3123	11534-11545	RB	denotes	transiently
T3124	11546-11556	VBG	denotes	expressing
T3125	11557-11558	DT	denotes	a
T3127	11559-11572	NN	denotes	hemagglutinin
T3129	11573-11574	-LRB-	denotes	(
T3130	11574-11576	NN	denotes	HA
T3131	11576-11577	-RRB-	denotes	)
T3132	11577-11578	HYPH	denotes	-
T3128	11578-11584	VBN	denotes	tagged
T3133	11585-11590	NN	denotes	Snail
T3126	11591-11598	NN	denotes	protein
T3134	11599-11600	-LRB-	denotes	(
T3136	11600-11606	NN	denotes	Figure
T3135	11607-11609	NN	denotes	1B
T3137	11609-11610	-RRB-	denotes	)
T3138	11610-11611	.	denotes	.
T3139	11611-11928	sentence	denotes	The antibody also recognized a band corresponding to the size of endogenous Snail (approximately 28 kDa) in lysates from embryonic mouse skin, the temporal appearance of which corresponded to the waves of hair follicle morphogenesis from E15.5 to newborn when over 90% of the hair on the mouse is formed (Figure 1B).
T3140	11612-11615	DT	denotes	The
T3141	11616-11624	NN	denotes	antibody
T3143	11625-11629	RB	denotes	also
T3142	11630-11640	VBD	denotes	recognized
T3144	11641-11642	DT	denotes	a
T3145	11643-11647	NN	denotes	band
T3146	11648-11661	VBG	denotes	corresponding
T3147	11662-11664	IN	denotes	to
T3148	11665-11668	DT	denotes	the
T3149	11669-11673	NN	denotes	size
T3150	11674-11676	IN	denotes	of
T3151	11677-11687	JJ	denotes	endogenous
T3152	11688-11693	NN	denotes	Snail
T3153	11694-11695	-LRB-	denotes	(
T3154	11695-11708	RB	denotes	approximately
T3155	11709-11711	CD	denotes	28
T3156	11712-11715	NN	denotes	kDa
T3157	11715-11716	-RRB-	denotes	)
T3158	11717-11719	IN	denotes	in
T3159	11720-11727	NNS	denotes	lysates
T3160	11728-11732	IN	denotes	from
T3161	11733-11742	JJ	denotes	embryonic
T3163	11743-11748	NN	denotes	mouse
T3162	11749-11753	NN	denotes	skin
T3164	11753-11755	,	denotes	,
T3165	11755-11758	DT	denotes	the
T3167	11759-11767	JJ	denotes	temporal
T3166	11768-11778	NN	denotes	appearance
T3169	11779-11781	IN	denotes	of
T3170	11782-11787	WDT	denotes	which
T3168	11788-11800	VBD	denotes	corresponded
T3171	11801-11803	IN	denotes	to
T3172	11804-11807	DT	denotes	the
T3173	11808-11813	NNS	denotes	waves
T3174	11814-11816	IN	denotes	of
T3175	11817-11821	NN	denotes	hair
T3176	11822-11830	NN	denotes	follicle
T3177	11831-11844	NN	denotes	morphogenesis
T3178	11845-11849	IN	denotes	from
T3179	11850-11855	NN	denotes	E15.5
T3180	11856-11858	IN	denotes	to
T3181	11859-11866	JJ	denotes	newborn
T3182	11867-11871	WRB	denotes	when
T3184	11872-11876	IN	denotes	over
T3185	11877-11879	CD	denotes	90
T3186	11879-11880	NN	denotes	%
T3187	11881-11883	IN	denotes	of
T3188	11884-11887	DT	denotes	the
T3189	11888-11892	NN	denotes	hair
T3190	11893-11895	IN	denotes	on
T3191	11896-11899	DT	denotes	the
T3192	11900-11905	NN	denotes	mouse
T3193	11906-11908	VBZ	denotes	is
T3183	11909-11915	VBN	denotes	formed
T3194	11916-11917	-LRB-	denotes	(
T3196	11917-11923	NN	denotes	Figure
T3195	11924-11926	NN	denotes	1B
T3197	11926-11927	-RRB-	denotes	)
T3198	11927-11928	.	denotes	.
T3199	11928-12222	sentence	denotes	Consistent with the Western blot data, immunohistochemical analysis did not detect Snail in single-layered E13.5 epidermis (Figure 1C) nor in the placode, which is the earliest morphological sign of the commitment of multipotent cells of the embryonic ectoderm to a hair cell fate (Figure 1D).
T3200	11929-11939	JJ	denotes	Consistent
T3202	11940-11944	IN	denotes	with
T3203	11945-11948	DT	denotes	the
T3205	11949-11956	NNP	denotes	Western
T3206	11957-11961	NN	denotes	blot
T3204	11962-11966	NNS	denotes	data
T3207	11966-11968	,	denotes	,
T3208	11968-11987	JJ	denotes	immunohistochemical
T3209	11988-11996	NN	denotes	analysis
T3210	11997-12000	VBD	denotes	did
T3211	12001-12004	RB	denotes	not
T3201	12005-12011	VB	denotes	detect
T3212	12012-12017	NN	denotes	Snail
T3213	12018-12020	IN	denotes	in
T3214	12021-12027	JJ	denotes	single
T3216	12027-12028	HYPH	denotes	-
T3215	12028-12035	JJ	denotes	layered
T3218	12036-12041	NN	denotes	E13.5
T3217	12042-12051	NN	denotes	epidermis
T3219	12052-12053	-LRB-	denotes	(
T3221	12053-12059	NN	denotes	Figure
T3220	12060-12062	NN	denotes	1C
T3222	12062-12063	-RRB-	denotes	)
T3223	12064-12067	CC	denotes	nor
T3224	12068-12070	IN	denotes	in
T3225	12071-12074	DT	denotes	the
T3226	12075-12082	NN	denotes	placode
T3227	12082-12084	,	denotes	,
T3228	12084-12089	WDT	denotes	which
T3229	12090-12092	VBZ	denotes	is
T3230	12093-12096	DT	denotes	the
T3232	12097-12105	JJS	denotes	earliest
T3233	12106-12119	JJ	denotes	morphological
T3231	12120-12124	NN	denotes	sign
T3234	12125-12127	IN	denotes	of
T3235	12128-12131	DT	denotes	the
T3236	12132-12142	NN	denotes	commitment
T3237	12143-12145	IN	denotes	of
T3238	12146-12157	JJ	denotes	multipotent
T3239	12158-12163	NNS	denotes	cells
T3240	12164-12166	IN	denotes	of
T3241	12167-12170	DT	denotes	the
T3243	12171-12180	JJ	denotes	embryonic
T3242	12181-12189	NN	denotes	ectoderm
T3244	12190-12192	IN	denotes	to
T3245	12193-12194	DT	denotes	a
T3247	12195-12199	NN	denotes	hair
T3248	12200-12204	NN	denotes	cell
T3246	12205-12209	NN	denotes	fate
T3249	12210-12211	-LRB-	denotes	(
T3251	12211-12217	NN	denotes	Figure
T3250	12218-12220	NN	denotes	1D
T3252	12220-12221	-RRB-	denotes	)
T3253	12221-12222	.	denotes	.
T3254	12222-12384	sentence	denotes	Consistent with the in situ hybridization results, anti-Snail antibody labeled only hair buds and not follicles at more mature stages of development (Figure 1E).
T3255	12223-12233	JJ	denotes	Consistent
T3257	12234-12238	IN	denotes	with
T3258	12239-12242	DT	denotes	the
T3260	12243-12245	FW	denotes	in
T3261	12246-12250	FW	denotes	situ
T3262	12251-12264	NN	denotes	hybridization
T3259	12265-12272	NNS	denotes	results
T3263	12272-12274	,	denotes	,
T3264	12274-12284	JJ	denotes	anti-Snail
T3265	12285-12293	NN	denotes	antibody
T3256	12294-12301	VBD	denotes	labeled
T3266	12302-12306	RB	denotes	only
T3268	12307-12311	NN	denotes	hair
T3267	12312-12316	NNS	denotes	buds
T3269	12317-12320	CC	denotes	and
T3270	12321-12324	RB	denotes	not
T3271	12325-12334	NNS	denotes	follicles
T3272	12335-12337	IN	denotes	at
T3273	12338-12342	RBR	denotes	more
T3275	12343-12349	JJ	denotes	mature
T3274	12350-12356	NNS	denotes	stages
T3276	12357-12359	IN	denotes	of
T3277	12360-12371	NN	denotes	development
T3278	12372-12373	-LRB-	denotes	(
T3280	12373-12379	NN	denotes	Figure
T3279	12380-12382	NN	denotes	1E
T3281	12382-12383	-RRB-	denotes	)
T3282	12383-12384	.	denotes	.
T3283	12384-12472	sentence	denotes	Taken together, the anti-Snail antibody appeared to be specific for its target protein.
T3284	12385-12390	VBN	denotes	Taken
T3286	12391-12399	RB	denotes	together
T3287	12399-12401	,	denotes	,
T3288	12401-12404	DT	denotes	the
T3290	12405-12415	JJ	denotes	anti-Snail
T3289	12416-12424	NN	denotes	antibody
T3285	12425-12433	VBD	denotes	appeared
T3291	12434-12436	TO	denotes	to
T3292	12437-12439	VB	denotes	be
T3293	12440-12448	JJ	denotes	specific
T3294	12449-12452	IN	denotes	for
T3295	12453-12456	PRP$	denotes	its
T3297	12457-12463	NN	denotes	target
T3296	12464-12471	NN	denotes	protein
T3298	12471-12472	.	denotes	.
T3299	12472-12588	sentence	denotes	It did not detect other Snail family members known to be expressed in keratinocytes and/or skin (unpublished data).
T3300	12473-12475	PRP	denotes	It
T3302	12476-12479	VBD	denotes	did
T3303	12480-12483	RB	denotes	not
T3301	12484-12490	VB	denotes	detect
T3304	12491-12496	JJ	denotes	other
T3306	12497-12502	NN	denotes	Snail
T3307	12503-12509	NN	denotes	family
T3305	12510-12517	NNS	denotes	members
T3308	12518-12523	VBN	denotes	known
T3309	12524-12526	TO	denotes	to
T3311	12527-12529	VB	denotes	be
T3310	12530-12539	VBN	denotes	expressed
T3312	12540-12542	IN	denotes	in
T3313	12543-12556	NNS	denotes	keratinocytes
T3314	12557-12560	CC	denotes	and
T3315	12560-12561	HYPH	denotes	/
T3316	12561-12563	CC	denotes	or
T3317	12564-12568	NN	denotes	skin
T3318	12569-12570	-LRB-	denotes	(
T3320	12570-12581	JJ	denotes	unpublished
T3319	12582-12586	NNS	denotes	data
T3321	12586-12587	-RRB-	denotes	)
T3322	12587-12588	.	denotes	.
T3323	12588-12750	sentence	denotes	Furthermore, the immunohistochemical data paralleled our Snail in situ hybridization data revealing transient Snail expression at the hair bud stage (Figure 1A).
T3324	12589-12600	RB	denotes	Furthermore
T3326	12600-12602	,	denotes	,
T3327	12602-12605	DT	denotes	the
T3329	12606-12625	JJ	denotes	immunohistochemical
T3328	12626-12630	NNS	denotes	data
T3325	12631-12641	VBD	denotes	paralleled
T3330	12642-12645	PRP$	denotes	our
T3332	12646-12651	NN	denotes	Snail
T3334	12652-12654	FW	denotes	in
T3335	12655-12659	FW	denotes	situ
T3333	12660-12673	NN	denotes	hybridization
T3331	12674-12678	NNS	denotes	data
T3336	12679-12688	VBG	denotes	revealing
T3337	12689-12698	JJ	denotes	transient
T3339	12699-12704	NN	denotes	Snail
T3338	12705-12715	NN	denotes	expression
T3340	12716-12718	IN	denotes	at
T3341	12719-12722	DT	denotes	the
T3343	12723-12727	NN	denotes	hair
T3344	12728-12731	NN	denotes	bud
T3342	12732-12737	NN	denotes	stage
T3345	12738-12739	-LRB-	denotes	(
T3347	12739-12745	NN	denotes	Figure
T3346	12746-12748	NN	denotes	1A
T3348	12748-12749	-RRB-	denotes	)
T3349	12749-12750	.	denotes	.
T3350	12750-12862	sentence	denotes	As judged by immunohistochemistry, Snail protein was localized to the nuclei of the hair bud cells (Figure 1E).
T3351	12751-12753	IN	denotes	As
T3352	12754-12760	VBN	denotes	judged
T3354	12761-12763	IN	denotes	by
T3355	12764-12784	NN	denotes	immunohistochemistry
T3356	12784-12786	,	denotes	,
T3357	12786-12791	NN	denotes	Snail
T3358	12792-12799	NN	denotes	protein
T3359	12800-12803	VBD	denotes	was
T3353	12804-12813	VBN	denotes	localized
T3360	12814-12816	IN	denotes	to
T3361	12817-12820	DT	denotes	the
T3362	12821-12827	NNS	denotes	nuclei
T3363	12828-12830	IN	denotes	of
T3364	12831-12834	DT	denotes	the
T3366	12835-12839	NN	denotes	hair
T3367	12840-12843	NN	denotes	bud
T3365	12844-12849	NNS	denotes	cells
T3368	12850-12851	-LRB-	denotes	(
T3370	12851-12857	NN	denotes	Figure
T3369	12858-12860	NN	denotes	1E
T3371	12860-12861	-RRB-	denotes	)
T3372	12861-12862	.	denotes	.
T3373	12862-12958	sentence	denotes	This feature was consistent with Snail's known function as a transcriptional repressor [12,13].
T3374	12863-12867	DT	denotes	This
T3375	12868-12875	NN	denotes	feature
T3376	12876-12879	VBD	denotes	was
T3377	12880-12890	JJ	denotes	consistent
T3378	12891-12895	IN	denotes	with
T3379	12896-12901	NN	denotes	Snail
T3381	12901-12903	POS	denotes	's
T3382	12904-12909	VBN	denotes	known
T3380	12910-12918	NN	denotes	function
T3383	12919-12921	IN	denotes	as
T3384	12922-12923	DT	denotes	a
T3386	12924-12939	JJ	denotes	transcriptional
T3385	12940-12949	NN	denotes	repressor
T3387	12950-12951	-LRB-	denotes	[
T3389	12951-12953	CD	denotes	12
T3390	12953-12954	,	denotes	,
T3388	12954-12956	CD	denotes	13
T3391	12956-12957	-RRB-	denotes	]
T3392	12957-12958	.	denotes	.
T3393	12958-13070	sentence	denotes	Additionally, anti-Snail labeling was detected in only three of the four major waves of follicle morphogenesis.
T3394	12959-12971	RB	denotes	Additionally
T3396	12971-12973	,	denotes	,
T3397	12973-12983	JJ	denotes	anti-Snail
T3398	12984-12992	NN	denotes	labeling
T3399	12993-12996	VBD	denotes	was
T3395	12997-13005	VBN	denotes	detected
T3400	13006-13008	IN	denotes	in
T3401	13009-13013	RB	denotes	only
T3403	13014-13019	CD	denotes	three
T3404	13020-13022	IN	denotes	of
T3405	13023-13026	DT	denotes	the
T3402	13027-13031	CD	denotes	four
T3407	13032-13037	JJ	denotes	major
T3406	13038-13043	NNS	denotes	waves
T3408	13044-13046	IN	denotes	of
T3409	13047-13055	NN	denotes	follicle
T3410	13056-13069	NN	denotes	morphogenesis
T3411	13069-13070	.	denotes	.
T3412	13070-13244	sentence	denotes	Snail was not found in the buds of guard hairs that are the earliest of all hairs to form (at E13.5), and which constitute less than 5% of the mouse coat (unpublished data).
T3413	13071-13076	NN	denotes	Snail
T3415	13077-13080	VBD	denotes	was
T3416	13081-13084	RB	denotes	not
T3414	13085-13090	VBN	denotes	found
T3417	13091-13093	IN	denotes	in
T3418	13094-13097	DT	denotes	the
T3419	13098-13102	NNS	denotes	buds
T3420	13103-13105	IN	denotes	of
T3421	13106-13111	NN	denotes	guard
T3422	13112-13117	NNS	denotes	hairs
T3423	13118-13122	WDT	denotes	that
T3424	13123-13126	VBP	denotes	are
T3425	13127-13130	DT	denotes	the
T3426	13131-13139	JJS	denotes	earliest
T3427	13140-13142	IN	denotes	of
T3428	13143-13146	DT	denotes	all
T3429	13147-13152	NNS	denotes	hairs
T3430	13153-13155	TO	denotes	to
T3431	13156-13160	VB	denotes	form
T3432	13161-13162	-LRB-	denotes	(
T3433	13162-13164	IN	denotes	at
T3434	13165-13170	NN	denotes	E13.5
T3435	13170-13171	-RRB-	denotes	)
T3436	13171-13173	,	denotes	,
T3437	13173-13176	CC	denotes	and
T3438	13177-13182	WDT	denotes	which
T3439	13183-13193	VBP	denotes	constitute
T3440	13194-13198	JJR	denotes	less
T3442	13199-13203	IN	denotes	than
T3441	13204-13205	CD	denotes	5
T3443	13205-13206	NN	denotes	%
T3444	13207-13209	IN	denotes	of
T3445	13210-13213	DT	denotes	the
T3447	13214-13219	NN	denotes	mouse
T3446	13220-13224	NN	denotes	coat
T3448	13225-13226	-LRB-	denotes	(
T3450	13226-13237	JJ	denotes	unpublished
T3449	13238-13242	NNS	denotes	data
T3451	13242-13243	-RRB-	denotes	)
T3452	13243-13244	.	denotes	.
T3453	13244-13485	sentence	denotes	As judged by immunofluorescence with antibodies against the proliferating nuclear antigen Ki67, the timing of Snail expression coincided with the stage at which the developing follicle enhanced its proliferation and down-growth (Figure 1F).
T3454	13245-13247	IN	denotes	As
T3455	13248-13254	VBN	denotes	judged
T3457	13255-13257	IN	denotes	by
T3458	13258-13276	NN	denotes	immunofluorescence
T3459	13277-13281	IN	denotes	with
T3460	13282-13292	NNS	denotes	antibodies
T3461	13293-13300	IN	denotes	against
T3462	13301-13304	DT	denotes	the
T3464	13305-13318	VBG	denotes	proliferating
T3465	13319-13326	JJ	denotes	nuclear
T3463	13327-13334	NN	denotes	antigen
T3466	13335-13339	NN	denotes	Ki67
T3467	13339-13341	,	denotes	,
T3468	13341-13344	DT	denotes	the
T3469	13345-13351	NN	denotes	timing
T3470	13352-13354	IN	denotes	of
T3471	13355-13360	NN	denotes	Snail
T3472	13361-13371	NN	denotes	expression
T3456	13372-13381	VBD	denotes	coincided
T3473	13382-13386	IN	denotes	with
T3474	13387-13390	DT	denotes	the
T3475	13391-13396	NN	denotes	stage
T3476	13397-13399	IN	denotes	at
T3478	13400-13405	WDT	denotes	which
T3479	13406-13409	DT	denotes	the
T3481	13410-13420	VBG	denotes	developing
T3480	13421-13429	NN	denotes	follicle
T3477	13430-13438	VBD	denotes	enhanced
T3482	13439-13442	PRP$	denotes	its
T3483	13443-13456	NN	denotes	proliferation
T3484	13457-13460	CC	denotes	and
T3485	13461-13465	JJ	denotes	down
T3487	13465-13466	HYPH	denotes	-
T3486	13466-13472	NN	denotes	growth
T3488	13473-13474	-LRB-	denotes	(
T3490	13474-13480	NN	denotes	Figure
T3489	13481-13483	NN	denotes	1F
T3491	13483-13484	-RRB-	denotes	)
T3492	13484-13485	.	denotes	.
T3493	13485-13707	sentence	denotes	Immunohistochemistry with antibodies against the active (phosphorylated) form of MAPK (pMAPK) marked a subset of the proliferating (Ki67-positive) cells, and pMAPK-positive cells were enriched in the hair bud (Figure 1G).
T3494	13486-13506	NN	denotes	Immunohistochemistry
T3496	13507-13511	IN	denotes	with
T3497	13512-13522	NNS	denotes	antibodies
T3498	13523-13530	IN	denotes	against
T3499	13531-13534	DT	denotes	the
T3501	13535-13541	JJ	denotes	active
T3502	13542-13543	-LRB-	denotes	(
T3503	13543-13557	VBN	denotes	phosphorylated
T3504	13557-13558	-RRB-	denotes	)
T3500	13559-13563	NN	denotes	form
T3505	13564-13566	IN	denotes	of
T3506	13567-13571	NN	denotes	MAPK
T3507	13572-13573	-LRB-	denotes	(
T3508	13573-13578	NN	denotes	pMAPK
T3509	13578-13579	-RRB-	denotes	)
T3495	13580-13586	VBD	denotes	marked
T3510	13587-13588	DT	denotes	a
T3511	13589-13595	NN	denotes	subset
T3512	13596-13598	IN	denotes	of
T3513	13599-13602	DT	denotes	the
T3515	13603-13616	VBG	denotes	proliferating
T3516	13617-13618	-LRB-	denotes	(
T3517	13618-13622	NN	denotes	Ki67
T3519	13622-13623	HYPH	denotes	-
T3518	13623-13631	JJ	denotes	positive
T3520	13631-13632	-RRB-	denotes	)
T3514	13633-13638	NNS	denotes	cells
T3521	13638-13640	,	denotes	,
T3522	13640-13643	CC	denotes	and
T3523	13644-13649	NN	denotes	pMAPK
T3525	13649-13650	HYPH	denotes	-
T3524	13650-13658	JJ	denotes	positive
T3526	13659-13664	NNS	denotes	cells
T3528	13665-13669	VBD	denotes	were
T3527	13670-13678	VBN	denotes	enriched
T3529	13679-13681	IN	denotes	in
T3530	13682-13685	DT	denotes	the
T3532	13686-13690	NN	denotes	hair
T3531	13691-13694	NN	denotes	bud
T3533	13695-13696	-LRB-	denotes	(
T3535	13696-13702	NN	denotes	Figure
T3534	13703-13705	NN	denotes	1G
T3536	13705-13706	-RRB-	denotes	)
T3537	13706-13707	.	denotes	.
T3538	13707-13903	sentence	denotes	The timing of Snail induction and Ki67 and pMAPK enrichment in the hair bud appeared to follow closely the induction of LEF-1/β-catenin activity, known to initiate in the hair placode stage [20].
T3539	13708-13711	DT	denotes	The
T3540	13712-13718	NN	denotes	timing
T3542	13719-13721	IN	denotes	of
T3543	13722-13727	NN	denotes	Snail
T3544	13728-13737	NN	denotes	induction
T3545	13738-13741	CC	denotes	and
T3546	13742-13746	NN	denotes	Ki67
T3548	13747-13750	CC	denotes	and
T3549	13751-13756	NN	denotes	pMAPK
T3547	13757-13767	NN	denotes	enrichment
T3550	13768-13770	IN	denotes	in
T3551	13771-13774	DT	denotes	the
T3553	13775-13779	NN	denotes	hair
T3552	13780-13783	NN	denotes	bud
T3541	13784-13792	VBD	denotes	appeared
T3554	13793-13795	TO	denotes	to
T3555	13796-13802	VB	denotes	follow
T3556	13803-13810	RB	denotes	closely
T3557	13811-13814	DT	denotes	the
T3558	13815-13824	NN	denotes	induction
T3559	13825-13827	IN	denotes	of
T3560	13828-13831	NN	denotes	LEF
T3562	13831-13832	HYPH	denotes	-
T3563	13832-13833	CD	denotes	1
T3564	13833-13834	HYPH	denotes	/
T3565	13834-13835	NN	denotes	β
T3567	13835-13836	HYPH	denotes	-
T3566	13836-13843	NN	denotes	catenin
T3561	13844-13852	NN	denotes	activity
T3568	13852-13854	,	denotes	,
T3569	13854-13859	VBN	denotes	known
T3570	13860-13862	TO	denotes	to
T3571	13863-13871	VB	denotes	initiate
T3572	13872-13874	IN	denotes	in
T3573	13875-13878	DT	denotes	the
T3575	13879-13883	NN	denotes	hair
T3576	13884-13891	NN	denotes	placode
T3574	13892-13897	NN	denotes	stage
T3577	13898-13899	-LRB-	denotes	[
T3578	13899-13901	CD	denotes	20
T3579	13901-13902	-RRB-	denotes	]
T3580	13902-13903	.	denotes	.
T3581	13903-14015	sentence	denotes	However, like placodes, hair buds exhibited down-regulation in E-cadherin expression (Figure 1H; see also [4]).
T3582	13904-13911	RB	denotes	However
T3584	13911-13913	,	denotes	,
T3585	13913-13917	IN	denotes	like
T3586	13918-13926	NNS	denotes	placodes
T3587	13926-13928	,	denotes	,
T3588	13928-13932	NN	denotes	hair
T3589	13933-13937	NNS	denotes	buds
T3583	13938-13947	VBD	denotes	exhibited
T3590	13948-13952	JJ	denotes	down
T3592	13952-13953	HYPH	denotes	-
T3591	13953-13963	NN	denotes	regulation
T3593	13964-13966	IN	denotes	in
T3594	13967-13968	NN	denotes	E
T3596	13968-13969	HYPH	denotes	-
T3595	13969-13977	NN	denotes	cadherin
T3597	13978-13988	NN	denotes	expression
T3598	13989-13990	-LRB-	denotes	(
T3600	13990-13996	NN	denotes	Figure
T3599	13997-13999	NN	denotes	1H
T3601	13999-14000	:	denotes	;
T3602	14001-14004	VB	denotes	see
T3603	14005-14009	RB	denotes	also
T3604	14010-14011	-LRB-	denotes	[
T3605	14011-14012	CD	denotes	4
T3606	14012-14013	-RRB-	denotes	]
T3607	14013-14014	-RRB-	denotes	)
T3608	14014-14015	.	denotes	.
T3910	14017-14026	JJ	denotes	Sustained
T3911	14027-14037	NN	denotes	Expression
T3913	14038-14040	IN	denotes	of
T3914	14041-14046	NN	denotes	Snail
T3912	14047-14054	VBZ	denotes	Results
T3915	14055-14057	IN	denotes	in
T3916	14058-14067	JJ	denotes	Epidermal
T3917	14068-14086	NN	denotes	Hyperproliferation
T3918	14087-14090	CC	denotes	and
T3919	14091-14106	NN	denotes	Differentiation
T3920	14107-14114	NNS	denotes	Defects
T3921	14115-14117	IN	denotes	in
T3922	14118-14120	NN	denotes	Tg
T3924	14121-14126	NN	denotes	Mouse
T3923	14127-14131	NN	denotes	Skin
T3925	14131-14339	sentence	denotes	The striking spike of Snail expression coincident with hair bud formation and enhanced proliferation prompted us to examine the consequences of ectopically expressing Snail elsewhere in mouse skin epidermis.
T3926	14132-14135	DT	denotes	The
T3928	14136-14144	JJ	denotes	striking
T3927	14145-14150	NN	denotes	spike
T3930	14151-14153	IN	denotes	of
T3931	14154-14159	NN	denotes	Snail
T3932	14160-14170	NN	denotes	expression
T3933	14171-14181	JJ	denotes	coincident
T3934	14182-14186	IN	denotes	with
T3935	14187-14191	NN	denotes	hair
T3936	14192-14195	NN	denotes	bud
T3937	14196-14205	NN	denotes	formation
T3938	14206-14209	CC	denotes	and
T3939	14210-14218	VBN	denotes	enhanced
T3940	14219-14232	NN	denotes	proliferation
T3929	14233-14241	VBD	denotes	prompted
T3941	14242-14244	PRP	denotes	us
T3942	14245-14247	TO	denotes	to
T3943	14248-14255	VB	denotes	examine
T3944	14256-14259	DT	denotes	the
T3945	14260-14272	NNS	denotes	consequences
T3946	14273-14275	IN	denotes	of
T3947	14276-14287	RB	denotes	ectopically
T3948	14288-14298	VBG	denotes	expressing
T3949	14299-14304	NN	denotes	Snail
T3950	14305-14314	RB	denotes	elsewhere
T3951	14315-14317	IN	denotes	in
T3952	14318-14323	NN	denotes	mouse
T3954	14324-14328	NN	denotes	skin
T3953	14329-14338	NN	denotes	epidermis
T3955	14338-14339	.	denotes	.
T3956	14339-14473	sentence	denotes	To distinguish Tg from endogenous Snail, we used the HA-epitope, shown previously not to alter Snail's transcriptional activity [12].
T3957	14340-14342	TO	denotes	To
T3958	14343-14354	VB	denotes	distinguish
T3960	14355-14357	NN	denotes	Tg
T3961	14358-14362	IN	denotes	from
T3962	14363-14373	JJ	denotes	endogenous
T3963	14374-14379	NN	denotes	Snail
T3964	14379-14381	,	denotes	,
T3965	14381-14383	PRP	denotes	we
T3959	14384-14388	VBD	denotes	used
T3966	14389-14392	DT	denotes	the
T3968	14393-14395	NN	denotes	HA
T3969	14395-14396	HYPH	denotes	-
T3967	14396-14403	NN	denotes	epitope
T3970	14403-14405	,	denotes	,
T3971	14405-14410	VBN	denotes	shown
T3972	14411-14421	RB	denotes	previously
T3973	14422-14425	RB	denotes	not
T3975	14426-14428	TO	denotes	to
T3974	14429-14434	VB	denotes	alter
T3976	14435-14440	NNP	denotes	Snail
T3978	14440-14442	POS	denotes	's
T3979	14443-14458	JJ	denotes	transcriptional
T3977	14459-14467	NN	denotes	activity
T3980	14468-14469	-LRB-	denotes	[
T3981	14469-14471	CD	denotes	12
T3982	14471-14472	-RRB-	denotes	]
T3983	14472-14473	.	denotes	.
T3984	14473-14585	sentence	denotes	Of 20 K14-Snail[HA] Tg animals generated, three expressed the transgene and all exhibited analogous phenotypes.
T3985	14474-14476	IN	denotes	Of
T3987	14477-14479	CD	denotes	20
T3989	14480-14483	NN	denotes	K14
T3991	14483-14484	HYPH	denotes	-
T3990	14484-14489	NN	denotes	Snail
T3992	14489-14490	-LRB-	denotes	[
T3993	14490-14492	NN	denotes	HA
T3994	14492-14493	-RRB-	denotes	]
T3995	14494-14496	NN	denotes	Tg
T3988	14497-14504	NNS	denotes	animals
T3996	14505-14514	VBN	denotes	generated
T3997	14514-14516	,	denotes	,
T3998	14516-14521	CD	denotes	three
T3986	14522-14531	VBD	denotes	expressed
T3999	14532-14535	DT	denotes	the
T4000	14536-14545	NN	denotes	transgene
T4001	14546-14549	CC	denotes	and
T4002	14550-14553	DT	denotes	all
T4003	14554-14563	VBD	denotes	exhibited
T4004	14564-14573	JJ	denotes	analogous
T4005	14574-14584	NNS	denotes	phenotypes
T4006	14584-14585	.	denotes	.
T4007	14585-14677	sentence	denotes	Mice that integrated the transgene at the single-cell stage died at or shortly after birth.
T4008	14586-14590	NNS	denotes	Mice
T4010	14591-14595	WDT	denotes	that
T4011	14596-14606	VBD	denotes	integrated
T4012	14607-14610	DT	denotes	the
T4013	14611-14620	NN	denotes	transgene
T4014	14621-14623	IN	denotes	at
T4015	14624-14627	DT	denotes	the
T4017	14628-14634	JJ	denotes	single
T4019	14634-14635	HYPH	denotes	-
T4018	14635-14639	NN	denotes	cell
T4016	14640-14645	NN	denotes	stage
T4009	14646-14650	VBD	denotes	died
T4020	14651-14653	IN	denotes	at
T4021	14654-14656	CC	denotes	or
T4022	14657-14664	RB	denotes	shortly
T4023	14665-14670	IN	denotes	after
T4024	14671-14676	NN	denotes	birth
T4025	14676-14677	.	denotes	.
T4026	14677-14834	sentence	denotes	The three surviving full-Tg founder mice harbored transgene integrations that gave stable transmission of mosaic Snail gene expression through the germline.
T4027	14678-14681	DT	denotes	The
T4029	14682-14687	CD	denotes	three
T4030	14688-14697	VBG	denotes	surviving
T4031	14698-14702	JJ	denotes	full
T4033	14702-14703	HYPH	denotes	-
T4032	14703-14705	NN	denotes	Tg
T4034	14706-14713	NN	denotes	founder
T4028	14714-14718	NNS	denotes	mice
T4035	14719-14727	VBD	denotes	harbored
T4036	14728-14737	NN	denotes	transgene
T4037	14738-14750	NNS	denotes	integrations
T4038	14751-14755	WDT	denotes	that
T4039	14756-14760	VBD	denotes	gave
T4040	14761-14767	JJ	denotes	stable
T4041	14768-14780	NN	denotes	transmission
T4042	14781-14783	IN	denotes	of
T4043	14784-14790	JJ	denotes	mosaic
T4045	14791-14796	NN	denotes	Snail
T4044	14797-14801	NN	denotes	gene
T4046	14802-14812	NN	denotes	expression
T4047	14813-14820	IN	denotes	through
T4048	14821-14824	DT	denotes	the
T4049	14825-14833	NN	denotes	germline
T4050	14833-14834	.	denotes	.
T4051	14834-14945	sentence	denotes	Progressively poor health necessitated our sacrificing most offspring from these lines within a year of birth.
T4052	14835-14848	RB	denotes	Progressively
T4053	14849-14853	JJ	denotes	poor
T4054	14854-14860	NN	denotes	health
T4055	14861-14873	VBD	denotes	necessitated
T4056	14874-14877	PRP$	denotes	our
T4057	14878-14889	VBG	denotes	sacrificing
T4058	14890-14894	JJS	denotes	most
T4059	14895-14904	NN	denotes	offspring
T4060	14905-14909	IN	denotes	from
T4061	14910-14915	DT	denotes	these
T4062	14916-14921	NNS	denotes	lines
T4063	14922-14928	IN	denotes	within
T4064	14929-14930	DT	denotes	a
T4065	14931-14935	NN	denotes	year
T4066	14936-14938	IN	denotes	of
T4067	14939-14944	NN	denotes	birth
T4068	14944-14945	.	denotes	.
T4069	14945-15059	sentence	denotes	As Snail Tg animals grew, they became distinguished by their small size, short tails, and flaky skin (Figure 2A).
T4070	14946-14948	IN	denotes	As
T4072	14949-14954	NN	denotes	Snail
T4074	14955-14957	NN	denotes	Tg
T4073	14958-14965	NNS	denotes	animals
T4071	14966-14970	VBD	denotes	grew
T4076	14970-14972	,	denotes	,
T4077	14972-14976	PRP	denotes	they
T4075	14977-14983	VBD	denotes	became
T4078	14984-14997	JJ	denotes	distinguished
T4079	14998-15000	IN	denotes	by
T4080	15001-15006	PRP$	denotes	their
T4082	15007-15012	JJ	denotes	small
T4081	15013-15017	NN	denotes	size
T4083	15017-15019	,	denotes	,
T4084	15019-15024	JJ	denotes	short
T4085	15025-15030	NNS	denotes	tails
T4086	15030-15032	,	denotes	,
T4087	15032-15035	CC	denotes	and
T4088	15036-15041	JJ	denotes	flaky
T4089	15042-15046	NN	denotes	skin
T4090	15047-15048	-LRB-	denotes	(
T4092	15048-15054	NN	denotes	Figure
T4091	15055-15057	NN	denotes	2A
T4093	15057-15058	-RRB-	denotes	)
T4094	15058-15059	.	denotes	.
T4095	15059-15170	sentence	denotes	Histological analyses of 3-d old (P3) mice revealed mosaic patches marked by epidermal thickening (Figure 2B).
T4096	15060-15072	JJ	denotes	Histological
T4097	15073-15081	NNS	denotes	analyses
T4099	15082-15084	IN	denotes	of
T4100	15085-15086	CD	denotes	3
T4102	15086-15087	HYPH	denotes	-
T4101	15087-15088	NN	denotes	d
T4103	15089-15092	JJ	denotes	old
T4105	15093-15094	-LRB-	denotes	(
T4106	15094-15096	JJ	denotes	P3
T4107	15096-15097	-RRB-	denotes	)
T4104	15098-15102	NNS	denotes	mice
T4098	15103-15111	VBD	denotes	revealed
T4108	15112-15118	NN	denotes	mosaic
T4109	15119-15126	NNS	denotes	patches
T4110	15127-15133	VBN	denotes	marked
T4111	15134-15136	IN	denotes	by
T4112	15137-15146	JJ	denotes	epidermal
T4113	15147-15157	NN	denotes	thickening
T4114	15158-15159	-LRB-	denotes	(
T4116	15159-15165	NN	denotes	Figure
T4115	15166-15168	NN	denotes	2B
T4117	15168-15169	-RRB-	denotes	)
T4118	15169-15170	.	denotes	.
T4119	15170-15325	sentence	denotes	The mosaic morphology was reflected at the level of Tg Snail protein, with only the hyperthickened regions expressing nuclear HA-tagged Snail (Figure 2C).
T4120	15171-15174	DT	denotes	The
T4122	15175-15181	NN	denotes	mosaic
T4121	15182-15192	NN	denotes	morphology
T4124	15193-15196	VBD	denotes	was
T4123	15197-15206	VBN	denotes	reflected
T4125	15207-15209	IN	denotes	at
T4126	15210-15213	DT	denotes	the
T4127	15214-15219	NN	denotes	level
T4128	15220-15222	IN	denotes	of
T4129	15223-15225	NN	denotes	Tg
T4131	15226-15231	NN	denotes	Snail
T4130	15232-15239	NN	denotes	protein
T4132	15239-15241	,	denotes	,
T4133	15241-15245	IN	denotes	with
T4135	15246-15250	RB	denotes	only
T4137	15251-15254	DT	denotes	the
T4138	15255-15269	VBN	denotes	hyperthickened
T4136	15270-15277	NNS	denotes	regions
T4134	15278-15288	VBG	denotes	expressing
T4139	15289-15296	JJ	denotes	nuclear
T4141	15297-15299	NN	denotes	HA
T4143	15299-15300	HYPH	denotes	-
T4142	15300-15306	VBN	denotes	tagged
T4140	15307-15312	NN	denotes	Snail
T4144	15313-15314	-LRB-	denotes	(
T4146	15314-15320	NN	denotes	Figure
T4145	15321-15323	NN	denotes	2C
T4147	15323-15324	-RRB-	denotes	)
T4148	15324-15325	.	denotes	.
T4149	15325-15485	sentence	denotes	These hyperthickened areas were marked by excessive proliferation, as revealed by antibodies against the proliferating nuclear antigen Ki67 (Figure 2D and 2E).
T4150	15326-15331	DT	denotes	These
T4152	15332-15346	VBN	denotes	hyperthickened
T4151	15347-15352	NNS	denotes	areas
T4154	15353-15357	VBD	denotes	were
T4153	15358-15364	VBN	denotes	marked
T4155	15365-15367	IN	denotes	by
T4156	15368-15377	JJ	denotes	excessive
T4157	15378-15391	NN	denotes	proliferation
T4158	15391-15393	,	denotes	,
T4159	15393-15395	IN	denotes	as
T4160	15396-15404	VBN	denotes	revealed
T4161	15405-15407	IN	denotes	by
T4162	15408-15418	NNS	denotes	antibodies
T4163	15419-15426	IN	denotes	against
T4164	15427-15430	DT	denotes	the
T4166	15431-15444	VBG	denotes	proliferating
T4167	15445-15452	JJ	denotes	nuclear
T4165	15453-15460	NN	denotes	antigen
T4168	15461-15465	NN	denotes	Ki67
T4169	15466-15467	-LRB-	denotes	(
T4171	15467-15473	NN	denotes	Figure
T4170	15474-15476	NN	denotes	2D
T4172	15477-15480	CC	denotes	and
T4173	15481-15483	NN	denotes	2E
T4174	15483-15484	-RRB-	denotes	)
T4175	15484-15485	.	denotes	.
T4176	15485-15698	sentence	denotes	Activated, pMAPK-positive cells were also prevalent in these areas (Figure 2F and 2G), as were cells expressing keratin 6, a keratin induced in the suprabasal layers of hyperproliferative skin (Figure 2H and 2I).
T4177	15486-15495	VBN	denotes	Activated
T4179	15495-15497	,	denotes	,
T4180	15497-15502	NN	denotes	pMAPK
T4182	15502-15503	HYPH	denotes	-
T4181	15503-15511	JJ	denotes	positive
T4178	15512-15517	NNS	denotes	cells
T4183	15518-15522	VBD	denotes	were
T4184	15523-15527	RB	denotes	also
T4185	15528-15537	JJ	denotes	prevalent
T4186	15538-15540	IN	denotes	in
T4187	15541-15546	DT	denotes	these
T4188	15547-15552	NNS	denotes	areas
T4189	15553-15554	-LRB-	denotes	(
T4191	15554-15560	NN	denotes	Figure
T4190	15561-15563	NN	denotes	2F
T4192	15564-15567	CC	denotes	and
T4193	15568-15570	NN	denotes	2G
T4194	15570-15571	-RRB-	denotes	)
T4195	15571-15573	,	denotes	,
T4196	15573-15575	IN	denotes	as
T4197	15576-15580	VBD	denotes	were
T4198	15581-15586	NNS	denotes	cells
T4199	15587-15597	VBG	denotes	expressing
T4200	15598-15605	NN	denotes	keratin
T4201	15606-15607	CD	denotes	6
T4202	15607-15609	,	denotes	,
T4203	15609-15610	DT	denotes	a
T4204	15611-15618	NN	denotes	keratin
T4205	15619-15626	VBN	denotes	induced
T4206	15627-15629	IN	denotes	in
T4207	15630-15633	DT	denotes	the
T4209	15634-15644	JJ	denotes	suprabasal
T4208	15645-15651	NNS	denotes	layers
T4210	15652-15654	IN	denotes	of
T4211	15655-15673	JJ	denotes	hyperproliferative
T4212	15674-15678	NN	denotes	skin
T4213	15679-15680	-LRB-	denotes	(
T4215	15680-15686	NN	denotes	Figure
T4214	15687-15689	NN	denotes	2H
T4216	15690-15693	CC	denotes	and
T4217	15694-15696	NN	denotes	2I
T4218	15696-15697	-RRB-	denotes	)
T4219	15697-15698	.	denotes	.
T4220	15698-17837	sentence	denotes	Figure 2 Misexpression of Snail in Mouse Skin Epidermis Results in Hyperproliferation Three different surviving Tg founder mice harbored a K14-Snail transgene that was integrated into a locus that resulted in inheritable, mosaic expression of the transgene in skin epidermis. All displayed similar abnormalities, as did their offspring. (A) P16 WT and K14-Snail Tg mice. Insets denote magnified tail segments, which displayed a mosaic, flaky appearance in Tg mice. Size differences appeared with age, and are likely due to K14-promoter activity in the tongue and oral epithelium, resulting in progressive defects and reduced food intake. Hence, skin sections from young (P3) mice were analyzed (B–I). (B) Hematoxylin- and eosin-stained Tg skin section. Double arrows demarcate the border of mosaic histology, with seemingly normal epidermis (epi) and a mature hair follicle (hf) at left and hyperthickened epidermis at right. (C) Immunofluorescence of Tg skin section labeled with antibodies as color-coded on frame. Double arrows demarcate the border of mosaic anti-Snail (green), revealing Snail expression coincident with regions of hyperthickened epidermis (at left) and absent in regions of normal epidermis (at right). (D–I) Sections of P3 WT or Tg skin (affected region) subjected to either immunofluorescence (D, E, H, and I) or immunohistochemistry (F and G) with antibodies as indicated on the panel. Anti-keratin 5 indicates K5, normally restricted to the basal layer of the epidermis; anti-keratin 6 detects keratin 6, expressed in postnatal epidermis under conditions such as wounding, in which hyperproliferation occurs. All other antibodies are as in the legend to Figure 2. Comparison of D and E provide representative examples that illustrate that pMAPK is found in only a subset of all proliferating (Ki67-positive) cells. Note also the presence of Ki67- (E) and pMAPK-positive (G) cells in some suprabasal areas; Ki67-positive cells colabeled with anti-Snail (E). Expression of the Snail transgene did not block terminal differentiation in the hyperproliferative epidermis, but it distorted it markedly (Figure 3A–3H).
T4221	17683-17693	NN	denotes	Expression
T4223	17694-17696	IN	denotes	of
T4224	17697-17700	DT	denotes	the
T4226	17701-17706	NN	denotes	Snail
T4225	17707-17716	NN	denotes	transgene
T4227	17717-17720	VBD	denotes	did
T4228	17721-17724	RB	denotes	not
T4222	17725-17730	VB	denotes	block
T4229	17731-17739	JJ	denotes	terminal
T4230	17740-17755	NN	denotes	differentiation
T4231	17756-17758	IN	denotes	in
T4232	17759-17762	DT	denotes	the
T4234	17763-17781	JJ	denotes	hyperproliferative
T4233	17782-17791	NN	denotes	epidermis
T4235	17791-17793	,	denotes	,
T4236	17793-17796	CC	denotes	but
T4237	17797-17799	PRP	denotes	it
T4238	17800-17809	VBD	denotes	distorted
T4239	17810-17812	PRP	denotes	it
T4240	17813-17821	RB	denotes	markedly
T4241	17822-17823	-LRB-	denotes	(
T4243	17823-17829	NN	denotes	Figure
T4242	17830-17832	NN	denotes	3A
T4244	17832-17833	SYM	denotes	–
T4245	17833-17835	NN	denotes	3H
T4246	17835-17836	-RRB-	denotes	)
T4247	17836-17837	.	denotes	.
T4248	17837-17974	sentence	denotes	Typical of most hyperproliferating conditions, Snail expression led to a large expansion in layers with spinous and granular morphology.
T4249	17838-17845	JJ	denotes	Typical
T4251	17846-17848	IN	denotes	of
T4252	17849-17853	JJS	denotes	most
T4254	17854-17872	NN	denotes	hyperproliferating
T4253	17873-17883	NNS	denotes	conditions
T4255	17883-17885	,	denotes	,
T4256	17885-17890	NN	denotes	Snail
T4257	17891-17901	NN	denotes	expression
T4250	17902-17905	VBD	denotes	led
T4258	17906-17908	IN	denotes	to
T4259	17909-17910	DT	denotes	a
T4261	17911-17916	JJ	denotes	large
T4260	17917-17926	NN	denotes	expansion
T4262	17927-17929	IN	denotes	in
T4263	17930-17936	NNS	denotes	layers
T4264	17937-17941	IN	denotes	with
T4265	17942-17949	JJ	denotes	spinous
T4267	17950-17953	CC	denotes	and
T4268	17954-17962	JJ	denotes	granular
T4266	17963-17973	NN	denotes	morphology
T4269	17973-17974	.	denotes	.
T4270	17974-18117	sentence	denotes	Additionally, however, was a marked and variable expansion of keratin 5 (K5), normally restricted to the innermost basal layer (see Figure 3).
T4271	17975-17987	RB	denotes	Additionally
T4273	17987-17989	,	denotes	,
T4274	17989-17996	RB	denotes	however
T4275	17996-17998	,	denotes	,
T4272	17998-18001	VBD	denotes	was
T4276	18002-18003	DT	denotes	a
T4278	18004-18010	JJ	denotes	marked
T4279	18011-18014	CC	denotes	and
T4280	18015-18023	JJ	denotes	variable
T4277	18024-18033	NN	denotes	expansion
T4281	18034-18036	IN	denotes	of
T4282	18037-18044	NN	denotes	keratin
T4283	18045-18046	CD	denotes	5
T4284	18047-18048	-LRB-	denotes	(
T4285	18048-18050	NN	denotes	K5
T4286	18050-18051	-RRB-	denotes	)
T4287	18051-18053	,	denotes	,
T4288	18053-18061	RB	denotes	normally
T4289	18062-18072	VBN	denotes	restricted
T4290	18073-18075	IN	denotes	to
T4291	18076-18079	DT	denotes	the
T4293	18080-18089	JJS	denotes	innermost
T4294	18090-18095	JJ	denotes	basal
T4292	18096-18101	NN	denotes	layer
T4295	18102-18103	-LRB-	denotes	(
T4296	18103-18106	VB	denotes	see
T4297	18107-18113	NN	denotes	Figure
T4298	18114-18115	CD	denotes	3
T4299	18115-18116	-RRB-	denotes	)
T4300	18116-18117	.	denotes	.
T4301	18117-18426	sentence	denotes	Although the failure of Snail-null mice to develop past gastrulation [21] precluded our ability to study the loss of Snail function in skin development, a good correlation emerged between the expression of Snail protein and the extension of K5, Ki67, and pMAPK suprabasally (compare data in Figures 2 and 3).
T4302	18118-18126	IN	denotes	Although
T4304	18127-18130	DT	denotes	the
T4305	18131-18138	NN	denotes	failure
T4306	18139-18141	IN	denotes	of
T4307	18142-18147	NN	denotes	Snail
T4309	18147-18148	HYPH	denotes	-
T4310	18148-18152	JJ	denotes	null
T4308	18153-18157	NNS	denotes	mice
T4311	18158-18160	TO	denotes	to
T4312	18161-18168	VB	denotes	develop
T4313	18169-18173	JJ	denotes	past
T4314	18174-18186	NN	denotes	gastrulation
T4315	18187-18188	-LRB-	denotes	[
T4316	18188-18190	CD	denotes	21
T4317	18190-18191	-RRB-	denotes	]
T4303	18192-18201	VBD	denotes	precluded
T4319	18202-18205	PRP$	denotes	our
T4320	18206-18213	NN	denotes	ability
T4321	18214-18216	TO	denotes	to
T4322	18217-18222	VB	denotes	study
T4323	18223-18226	DT	denotes	the
T4324	18227-18231	NN	denotes	loss
T4325	18232-18234	IN	denotes	of
T4326	18235-18240	NN	denotes	Snail
T4327	18241-18249	NN	denotes	function
T4328	18250-18252	IN	denotes	in
T4329	18253-18257	NN	denotes	skin
T4330	18258-18269	NN	denotes	development
T4331	18269-18271	,	denotes	,
T4332	18271-18272	DT	denotes	a
T4334	18273-18277	JJ	denotes	good
T4333	18278-18289	NN	denotes	correlation
T4318	18290-18297	VBD	denotes	emerged
T4335	18298-18305	IN	denotes	between
T4336	18306-18309	DT	denotes	the
T4337	18310-18320	NN	denotes	expression
T4338	18321-18323	IN	denotes	of
T4339	18324-18329	NN	denotes	Snail
T4340	18330-18337	NN	denotes	protein
T4341	18338-18341	CC	denotes	and
T4342	18342-18345	DT	denotes	the
T4343	18346-18355	NN	denotes	extension
T4344	18356-18358	IN	denotes	of
T4345	18359-18361	NN	denotes	K5
T4346	18361-18363	,	denotes	,
T4347	18363-18367	NN	denotes	Ki67
T4348	18367-18369	,	denotes	,
T4349	18369-18372	CC	denotes	and
T4350	18373-18378	NN	denotes	pMAPK
T4351	18379-18391	RB	denotes	suprabasally
T4352	18392-18393	-LRB-	denotes	(
T4353	18393-18400	VB	denotes	compare
T4354	18401-18405	NNS	denotes	data
T4355	18406-18408	IN	denotes	in
T4356	18409-18416	NNS	denotes	Figures
T4357	18417-18418	CD	denotes	2
T4358	18419-18422	CC	denotes	and
T4359	18423-18424	CD	denotes	3
T4360	18424-18425	-RRB-	denotes	)
T4361	18425-18426	.	denotes	.
T4362	18426-20408	sentence	denotes	Figure 3 Alterations in the Differentiation Program and Basement Membrane Organization in Snail-Expressing Tg Epidermis (A–H) Immunofluorescence of skin sections from P3 WT and Tg mice. Shown are affected areas of Tg skin; in areas where Snail protein was not expressed, stainings were normal. Sections were labeled with antibodies as indicated and color-coded on each frame. Antibodies are against markers of normal epidermal differentiation, and include K5 (a basally expressed keratin), K1 (a suprabasal keratin, expressed in spinous layer cells), involucrin (Inv; a suprabasally expressed cornified envelope protein found in upper spinous and granular layer cells), loricrin (Lor; a cornified envelope protein expressed in the granular layer), and filaggrin (Fil; a protein that bundles keratin filaments in the granular layer and stratum corneum). Note abnormal extension of anti-K5 suprabasally, often present in anti-K1 positive suprabasal Tg cells. (I–N) Immunohistochemistry (I and J) or immunofluorescence (K–N) of sections of P30 Wt (I, K, and M) and Tg (J, L, and N) (affected areas) skins using the antibodies indicated. Note that with age, affected areas of the Tg epidermis became increasingly undulating, often exhibiting papilloma-like invaginations (J). Insets in I and J are magnified views of the boxed areas, illustrating the absence (Wt) or presence (Tg) of nuclear anti-cyclin D staining. With age, affected areas of the Tg epidermis also displayed perturbations within the basement membrane, as judged by antibody labeling against either basement membrane (K and L) or hemidesmosomal (M and N) components. Double arrows in L demarcate mosaic zones, revealing that perturbations were restricted to hyperthickened, i.e., Snail-positive zones (to left of double arrows). Other abbreviations are as noted in the legend to Figure 2. The changes in hyperproliferation and differentiation were not initially accompanied by gross signs of epithelial invaginations.
T4363	20280-20283	DT	denotes	The
T4364	20284-20291	NNS	denotes	changes
T4366	20292-20294	IN	denotes	in
T4367	20295-20313	NN	denotes	hyperproliferation
T4368	20314-20317	CC	denotes	and
T4369	20318-20333	NN	denotes	differentiation
T4370	20334-20338	VBD	denotes	were
T4371	20339-20342	RB	denotes	not
T4372	20343-20352	RB	denotes	initially
T4365	20353-20364	VBN	denotes	accompanied
T4373	20365-20367	IN	denotes	by
T4374	20368-20373	JJ	denotes	gross
T4375	20374-20379	NNS	denotes	signs
T4376	20380-20382	IN	denotes	of
T4377	20383-20393	JJ	denotes	epithelial
T4378	20394-20407	NNS	denotes	invaginations
T4379	20407-20408	.	denotes	.
T4380	20408-20602	sentence	denotes	With age, however, epidermal folds and undulations developed in areas where Snail was expressed, and proliferative markers persisted in these regions (Figure 3I and 3J; anti-cyclin D staining).
T4381	20409-20413	IN	denotes	With
T4383	20414-20417	NN	denotes	age
T4384	20417-20419	,	denotes	,
T4385	20419-20426	RB	denotes	however
T4386	20426-20428	,	denotes	,
T4387	20428-20437	JJ	denotes	epidermal
T4388	20438-20443	NNS	denotes	folds
T4389	20444-20447	CC	denotes	and
T4390	20448-20459	NNS	denotes	undulations
T4382	20460-20469	VBN	denotes	developed
T4391	20470-20472	IN	denotes	in
T4392	20473-20478	NNS	denotes	areas
T4393	20479-20484	WRB	denotes	where
T4395	20485-20490	NN	denotes	Snail
T4396	20491-20494	VBD	denotes	was
T4394	20495-20504	VBN	denotes	expressed
T4397	20504-20506	,	denotes	,
T4398	20506-20509	CC	denotes	and
T4399	20510-20523	JJ	denotes	proliferative
T4400	20524-20531	NNS	denotes	markers
T4401	20532-20541	VBD	denotes	persisted
T4402	20542-20544	IN	denotes	in
T4403	20545-20550	DT	denotes	these
T4404	20551-20558	NNS	denotes	regions
T4405	20559-20560	-LRB-	denotes	(
T4407	20560-20566	NN	denotes	Figure
T4408	20567-20569	NN	denotes	3I
T4409	20570-20573	CC	denotes	and
T4410	20574-20576	NN	denotes	3J
T4411	20576-20577	:	denotes	;
T4412	20578-20589	JJ	denotes	anti-cyclin
T4413	20590-20591	NN	denotes	D
T4406	20592-20600	NN	denotes	staining
T4414	20600-20601	-RRB-	denotes	)
T4415	20601-20602	.	denotes	.
T4416	20602-20714	sentence	denotes	The undulations were accompanied by partial dissolution of the underlying basement membrane (Figure 3K and 3L).
T4417	20603-20606	DT	denotes	The
T4418	20607-20618	NNS	denotes	undulations
T4420	20619-20623	VBD	denotes	were
T4419	20624-20635	VBN	denotes	accompanied
T4421	20636-20638	IN	denotes	by
T4422	20639-20646	JJ	denotes	partial
T4423	20647-20658	NN	denotes	dissolution
T4424	20659-20661	IN	denotes	of
T4425	20662-20665	DT	denotes	the
T4427	20666-20676	JJ	denotes	underlying
T4428	20677-20685	NN	denotes	basement
T4426	20686-20694	NN	denotes	membrane
T4429	20695-20696	-LRB-	denotes	(
T4431	20696-20702	NN	denotes	Figure
T4430	20703-20705	NN	denotes	3K
T4432	20706-20709	CC	denotes	and
T4433	20710-20712	NN	denotes	3L
T4434	20712-20713	-RRB-	denotes	)
T4435	20713-20714	.	denotes	.
T4436	20714-20914	sentence	denotes	Aberrant staining was also observed with antibodies against components of the hemidesmosomes, which provide strong adhesion of basal epidermal cells to the underlying basal lamina (Figure 3M and 3N).
T4437	20715-20723	JJ	denotes	Aberrant
T4438	20724-20732	NN	denotes	staining
T4440	20733-20736	VBD	denotes	was
T4441	20737-20741	RB	denotes	also
T4439	20742-20750	VBN	denotes	observed
T4442	20751-20755	IN	denotes	with
T4443	20756-20766	NNS	denotes	antibodies
T4444	20767-20774	IN	denotes	against
T4445	20775-20785	NNS	denotes	components
T4446	20786-20788	IN	denotes	of
T4447	20789-20792	DT	denotes	the
T4448	20793-20807	NNS	denotes	hemidesmosomes
T4449	20807-20809	,	denotes	,
T4450	20809-20814	WDT	denotes	which
T4451	20815-20822	VBP	denotes	provide
T4452	20823-20829	JJ	denotes	strong
T4453	20830-20838	NN	denotes	adhesion
T4454	20839-20841	IN	denotes	of
T4455	20842-20847	JJ	denotes	basal
T4457	20848-20857	JJ	denotes	epidermal
T4456	20858-20863	NNS	denotes	cells
T4458	20864-20866	IN	denotes	to
T4459	20867-20870	DT	denotes	the
T4461	20871-20881	JJ	denotes	underlying
T4462	20882-20887	JJ	denotes	basal
T4460	20888-20894	NN	denotes	lamina
T4463	20895-20896	-LRB-	denotes	(
T4465	20896-20902	NN	denotes	Figure
T4464	20903-20905	NN	denotes	3M
T4466	20906-20909	CC	denotes	and
T4467	20910-20912	NN	denotes	3N
T4468	20912-20913	-RRB-	denotes	)
T4469	20913-20914	.	denotes	.
T4470	20914-21069	sentence	denotes	Interestingly, similar types of alterations occur in the basement membrane in the hair bud of embryonic and newborn mice when Snail is normally expressed.
T4471	20915-20928	RB	denotes	Interestingly
T4473	20928-20930	,	denotes	,
T4474	20930-20937	JJ	denotes	similar
T4475	20938-20943	NNS	denotes	types
T4476	20944-20946	IN	denotes	of
T4477	20947-20958	NNS	denotes	alterations
T4472	20959-20964	VBP	denotes	occur
T4478	20965-20967	IN	denotes	in
T4479	20968-20971	DT	denotes	the
T4481	20972-20980	NN	denotes	basement
T4480	20981-20989	NN	denotes	membrane
T4482	20990-20992	IN	denotes	in
T4483	20993-20996	DT	denotes	the
T4485	20997-21001	NN	denotes	hair
T4484	21002-21005	NN	denotes	bud
T4486	21006-21008	IN	denotes	of
T4487	21009-21018	JJ	denotes	embryonic
T4489	21019-21022	CC	denotes	and
T4490	21023-21030	JJ	denotes	newborn
T4488	21031-21035	NNS	denotes	mice
T4491	21036-21040	WRB	denotes	when
T4493	21041-21046	NN	denotes	Snail
T4494	21047-21049	VBZ	denotes	is
T4495	21050-21058	RB	denotes	normally
T4492	21059-21068	VBN	denotes	expressed
T4496	21068-21069	.	denotes	.
T4497	21069-21306	sentence	denotes	The fact that the basement membrane separating the epidermis from the dermis is altered only in the adult Tg animals suggests the involvement of intermediary factors not as readily available in the epidermis as they are in the follicle.
T4498	21070-21073	DT	denotes	The
T4499	21074-21078	NN	denotes	fact
T4501	21079-21083	IN	denotes	that
T4503	21084-21087	DT	denotes	the
T4505	21088-21096	NN	denotes	basement
T4504	21097-21105	NN	denotes	membrane
T4506	21106-21116	VBG	denotes	separating
T4507	21117-21120	DT	denotes	the
T4508	21121-21130	NN	denotes	epidermis
T4509	21131-21135	IN	denotes	from
T4510	21136-21139	DT	denotes	the
T4511	21140-21146	NN	denotes	dermis
T4512	21147-21149	VBZ	denotes	is
T4502	21150-21157	VBN	denotes	altered
T4513	21158-21162	RB	denotes	only
T4514	21163-21165	IN	denotes	in
T4515	21166-21169	DT	denotes	the
T4517	21170-21175	JJ	denotes	adult
T4518	21176-21178	NN	denotes	Tg
T4516	21179-21186	NNS	denotes	animals
T4500	21187-21195	VBZ	denotes	suggests
T4519	21196-21199	DT	denotes	the
T4520	21200-21211	NN	denotes	involvement
T4521	21212-21214	IN	denotes	of
T4522	21215-21227	JJ	denotes	intermediary
T4523	21228-21235	NNS	denotes	factors
T4524	21236-21239	RB	denotes	not
T4526	21240-21242	RB	denotes	as
T4527	21243-21250	RB	denotes	readily
T4525	21251-21260	JJ	denotes	available
T4528	21261-21263	IN	denotes	in
T4529	21264-21267	DT	denotes	the
T4530	21268-21277	NN	denotes	epidermis
T4531	21278-21280	IN	denotes	as
T4533	21281-21285	PRP	denotes	they
T4532	21286-21289	VBP	denotes	are
T4534	21290-21292	IN	denotes	in
T4535	21293-21296	DT	denotes	the
T4536	21297-21305	NN	denotes	follicle
T4537	21305-21306	.	denotes	.
T5218	21308-21316	JJ	denotes	Possible
T5219	21317-21322	NNS	denotes	Links
T5220	21323-21330	IN	denotes	between
T5221	21331-21340	JJ	denotes	Epidermal
T5222	21341-21359	NN	denotes	Hyperproliferation
T5223	21360-21363	CC	denotes	and
T5224	21364-21368	JJ	denotes	Down
T5226	21368-21369	HYPH	denotes	-
T5225	21369-21379	NN	denotes	regulation
T5227	21380-21382	IN	denotes	of
T5228	21383-21385	NN	denotes	AJ
T5229	21386-21394	NNS	denotes	Proteins
T5230	21395-21397	IN	denotes	in
T5231	21398-21403	NN	denotes	Snail
T5233	21404-21406	NN	denotes	Tg
T5232	21407-21411	NNS	denotes	Mice
T5234	21411-21731	sentence	denotes	Given that the E-cadherin promoter is a direct target for Snail-mediated repression in vitro [4,12,13], and that E-cadherin was down-regulated in Snail-expressing hair buds, we examined the status of E-cadherin and other AJ proteins within regions of hyperproliferative epidermis where Tg Snail was present (Figure 4A).
T5235	21412-21417	VBN	denotes	Given
T5237	21418-21422	IN	denotes	that
T5239	21423-21426	DT	denotes	the
T5241	21427-21428	NN	denotes	E
T5243	21428-21429	HYPH	denotes	-
T5242	21429-21437	NN	denotes	cadherin
T5240	21438-21446	NN	denotes	promoter
T5238	21447-21449	VBZ	denotes	is
T5244	21450-21451	DT	denotes	a
T5246	21452-21458	JJ	denotes	direct
T5245	21459-21465	NN	denotes	target
T5247	21466-21469	IN	denotes	for
T5248	21470-21475	NN	denotes	Snail
T5250	21475-21476	HYPH	denotes	-
T5249	21476-21484	VBN	denotes	mediated
T5251	21485-21495	NN	denotes	repression
T5252	21496-21498	FW	denotes	in
T5253	21499-21504	FW	denotes	vitro
T5254	21505-21506	-LRB-	denotes	[
T5256	21506-21507	CD	denotes	4
T5257	21507-21508	,	denotes	,
T5258	21508-21510	CD	denotes	12
T5259	21510-21511	,	denotes	,
T5255	21511-21513	CD	denotes	13
T5260	21513-21514	-RRB-	denotes	]
T5261	21514-21516	,	denotes	,
T5262	21516-21519	CC	denotes	and
T5263	21520-21524	IN	denotes	that
T5265	21525-21526	NN	denotes	E
T5267	21526-21527	HYPH	denotes	-
T5266	21527-21535	NN	denotes	cadherin
T5268	21536-21539	VBD	denotes	was
T5269	21540-21544	RB	denotes	down
T5270	21544-21545	HYPH	denotes	-
T5264	21545-21554	VBN	denotes	regulated
T5271	21555-21557	IN	denotes	in
T5272	21558-21563	NN	denotes	Snail
T5274	21563-21564	HYPH	denotes	-
T5273	21564-21574	VBG	denotes	expressing
T5276	21575-21579	NN	denotes	hair
T5275	21580-21584	NNS	denotes	buds
T5277	21584-21586	,	denotes	,
T5278	21586-21588	PRP	denotes	we
T5236	21589-21597	VBD	denotes	examined
T5279	21598-21601	DT	denotes	the
T5280	21602-21608	NN	denotes	status
T5281	21609-21611	IN	denotes	of
T5282	21612-21613	NN	denotes	E
T5284	21613-21614	HYPH	denotes	-
T5283	21614-21622	NN	denotes	cadherin
T5285	21623-21626	CC	denotes	and
T5286	21627-21632	JJ	denotes	other
T5288	21633-21635	NN	denotes	AJ
T5287	21636-21644	NN	denotes	proteins
T5289	21645-21651	IN	denotes	within
T5290	21652-21659	NNS	denotes	regions
T5291	21660-21662	IN	denotes	of
T5292	21663-21681	JJ	denotes	hyperproliferative
T5293	21682-21691	NN	denotes	epidermis
T5294	21692-21697	WRB	denotes	where
T5296	21698-21700	NN	denotes	Tg
T5297	21701-21706	NN	denotes	Snail
T5295	21707-21710	VBD	denotes	was
T5298	21711-21718	JJ	denotes	present
T5299	21719-21720	-LRB-	denotes	(
T5301	21720-21726	NN	denotes	Figure
T5300	21727-21729	NN	denotes	4A
T5302	21729-21730	-RRB-	denotes	)
T5303	21730-21731	.	denotes	.
T5304	21731-21831	sentence	denotes	In these regions, immunofluorescence staining of E-cadherin and α-catenin were markedly diminished.
T5305	21732-21734	IN	denotes	In
T5307	21735-21740	DT	denotes	these
T5308	21741-21748	NNS	denotes	regions
T5309	21748-21750	,	denotes	,
T5310	21750-21768	NN	denotes	immunofluorescence
T5311	21769-21777	NN	denotes	staining
T5312	21778-21780	IN	denotes	of
T5313	21781-21782	NN	denotes	E
T5315	21782-21783	HYPH	denotes	-
T5314	21783-21791	NN	denotes	cadherin
T5316	21792-21795	CC	denotes	and
T5317	21796-21797	NN	denotes	α
T5319	21797-21798	HYPH	denotes	-
T5318	21798-21805	NN	denotes	catenin
T5320	21806-21810	VBD	denotes	were
T5321	21811-21819	RB	denotes	markedly
T5306	21820-21830	VBN	denotes	diminished
T5322	21830-21831	.	denotes	.
T5323	21831-21945	sentence	denotes	In contrast, the intensity of antibody staining for two other AJ proteins, β-catenin and Ajuba, was still strong.
T5324	21832-21834	IN	denotes	In
T5326	21835-21843	NN	denotes	contrast
T5327	21843-21845	,	denotes	,
T5328	21845-21848	DT	denotes	the
T5329	21849-21858	NN	denotes	intensity
T5330	21859-21861	IN	denotes	of
T5331	21862-21870	NN	denotes	antibody
T5332	21871-21879	NN	denotes	staining
T5333	21880-21883	IN	denotes	for
T5334	21884-21887	CD	denotes	two
T5336	21888-21893	JJ	denotes	other
T5337	21894-21896	NN	denotes	AJ
T5335	21897-21905	NN	denotes	proteins
T5338	21905-21907	,	denotes	,
T5339	21907-21908	NN	denotes	β
T5341	21908-21909	HYPH	denotes	-
T5340	21909-21916	NN	denotes	catenin
T5342	21917-21920	CC	denotes	and
T5343	21921-21926	NN	denotes	Ajuba
T5344	21926-21928	,	denotes	,
T5325	21928-21931	VBD	denotes	was
T5345	21932-21937	RB	denotes	still
T5346	21938-21944	JJ	denotes	strong
T5347	21944-21945	.	denotes	.
T5348	21945-22133	sentence	denotes	Interestingly, however, despite appreciable immunofluorescence, localization of β-catenin and Ajuba appeared to be largely cytoplasmic rather than at cell-cell borders (Figure 4A insets).
T5349	21946-21959	RB	denotes	Interestingly
T5351	21959-21961	,	denotes	,
T5352	21961-21968	RB	denotes	however
T5353	21968-21970	,	denotes	,
T5354	21970-21977	IN	denotes	despite
T5355	21978-21989	JJ	denotes	appreciable
T5356	21990-22008	NN	denotes	immunofluorescence
T5357	22008-22010	,	denotes	,
T5358	22010-22022	NN	denotes	localization
T5359	22023-22025	IN	denotes	of
T5360	22026-22027	NN	denotes	β
T5362	22027-22028	HYPH	denotes	-
T5361	22028-22035	NN	denotes	catenin
T5363	22036-22039	CC	denotes	and
T5364	22040-22045	NN	denotes	Ajuba
T5350	22046-22054	VBD	denotes	appeared
T5365	22055-22057	TO	denotes	to
T5366	22058-22060	VB	denotes	be
T5367	22061-22068	RB	denotes	largely
T5368	22069-22080	JJ	denotes	cytoplasmic
T5369	22081-22087	RB	denotes	rather
T5370	22088-22092	IN	denotes	than
T5371	22093-22095	IN	denotes	at
T5372	22096-22100	NN	denotes	cell
T5374	22100-22101	HYPH	denotes	-
T5373	22101-22105	NN	denotes	cell
T5375	22106-22113	NNS	denotes	borders
T5376	22114-22115	-LRB-	denotes	(
T5378	22115-22121	NN	denotes	Figure
T5379	22122-22124	NN	denotes	4A
T5377	22125-22131	NNS	denotes	insets
T5380	22131-22132	-RRB-	denotes	)
T5381	22132-22133	.	denotes	.
T5382	22133-26210	sentence	denotes	Figure 4 Snail-Mediated Remodeling of AJs Contributes to Hyperproliferation (A) Immunofluorescence of skin sections from P30 Wt and Tg mice. Shown are affected areas of Tg skin; in areas where Snail protein was not expressed, stainings were normal. Antibodies used are against AJ proteins and include E-cadherin (E-cad), the transmembrane core protein; β-catenin (β-cat), which binds E-cadherin at AJs and which can also participate as a transcription cofactor when associated with LEF-1/TCF proteins in the nucleus; α-catenin (α-cat) which binds to both β-catenin and Ajuba, a close relative of zyxin; and Ajuba, which can associate with proteins that bind to the actin cytoskeleton, as well as with Grb-2, a mediator of the GTP nucleotide-exchange protein Sos, involved in activation of the Ras-MAPK signaling cascade. In Snail-expressing Tg regions, there was a reduced staining with anti-E-cad and anti-α-cat and a more diffuse staining with anti-Ajuba. Insets in the panels for β-catenin and Ajuba staining are magnified views of the boxed areas. Arrows mark membrane localization of the protein and asterisks mark cells with elevated levels of cytoplasmic β-catenin or Ajuba. (B) Western blot analyses of protein extracts from P30 Wt and Tg back and ear skins. Antibodies are as in (A) except anti-P-cad, which detects P-cadherin, whose expression in the hair follicle was not affected, and anti-tubulin, which detects tubulin, a control for equal protein loadings. Note that the reductions seen in E-cadherin and α-catenin are likely to be underestimates of the actual differences in affected regions, since the Tg skin expressed Snail mosaically. (C) In the presence of elevated Snail, α-catenin levels can be restored by overexpression of E-cadherin. Keratinocytes were transfected with either HA-tagged Snail (Snail[HA]; images on the left) or Snail(HA) and Ecad(HA) (images on the right). 2 d after transfection, cells were switched from low-calcium growth medium to high-calcium medium for 6 h to induce AJ formation. Cells were stained with antibodies as indicated on the panels. Arrowheads point to sites of intercellular contact between a Snail-transfected keratinocyte and its neighboring untransfected cell. (D) Reintroduction of E-cadherin in keratinocytes expressing Snail returns pMAPK to basal levels. Keratinocytes were transfected with control vector (K14), or Snail(HA), or Snail(HA) + E-cad(HA). After 2 d, cells were serum starved for 4 h and whole cell lysates were made and Western blotted with antibodies to pMAPK, HA to recognize the HA-tagged Snail and E-cadherin protein, 20or tubulin as a loading control. (E) Ajuba interacts with Grb-2 under conditions where α-catenin levels are reduced. Protein extracts were made from skins of P30 Wt and K14-Snail Tg P30 mice (blots on the left) and of newborn Wt and K14-Cre/α-catenin (fl/fl) conditionally null animals (blots on the right) [7]. Equal amounts of protein extracts were treated with anti-Grb-2 antibody (+) or control isotype antibody (–), and following centrifugation, immunoprecipitates were subjected to SDS-PAGE and Western blot analysis with anti-Ajuba and anti-Grb-2 antibodies. Note the presence of Ajuba only under conditions where levels of α-catenin and other AJ proteins were aberrantly low or absent. (F) Transgene expression of excess Ajuba or the Grb-2-interacting domain (pre-LIM) of Ajuba in keratinocytes results in the activation of the Ras-MAPK pathway. Primary newborn mouse keratinocytes were transfected with either the empty K14 expression vector (K14), or the expression vector driving Snail, full length Ajuba, or the pre-LIM domain of Ajuba in the absence or presence of a peptide inhibitor (inh) that disrupts the interaction between Grb-2 and Sos. 48 h posttransfection, protein extracts were prepared and subjected to SDS-PAGE and Western blot analyses with antibodies against pMAPK, total MAPK, Ajuba (also recognizing the smaller, pre-LIM domain), and Snail. Architectural differences in the epidermis made Western blot analyses somewhat difficult to gauge.
T5383	26112-26125	JJ	denotes	Architectural
T5384	26126-26137	NNS	denotes	differences
T5386	26138-26140	IN	denotes	in
T5387	26141-26144	DT	denotes	the
T5388	26145-26154	NN	denotes	epidermis
T5385	26155-26159	VBD	denotes	made
T5389	26160-26167	NNP	denotes	Western
T5390	26168-26172	NN	denotes	blot
T5391	26173-26181	NNS	denotes	analyses
T5393	26182-26190	RB	denotes	somewhat
T5392	26191-26200	JJ	denotes	difficult
T5394	26201-26203	TO	denotes	to
T5395	26204-26209	VB	denotes	gauge
T5396	26209-26210	.	denotes	.
T5397	26210-26336	sentence	denotes	However, in regions such as ear skin, where the highest levels of Snail protein were expressed, the effects were accentuated.
T5398	26211-26218	RB	denotes	However
T5400	26218-26220	,	denotes	,
T5401	26220-26222	IN	denotes	in
T5402	26223-26230	NNS	denotes	regions
T5403	26231-26235	JJ	denotes	such
T5404	26236-26238	IN	denotes	as
T5405	26239-26242	NN	denotes	ear
T5406	26243-26247	NN	denotes	skin
T5407	26247-26249	,	denotes	,
T5408	26249-26254	WRB	denotes	where
T5410	26255-26258	DT	denotes	the
T5412	26259-26266	JJS	denotes	highest
T5411	26267-26273	NNS	denotes	levels
T5413	26274-26276	IN	denotes	of
T5414	26277-26282	NN	denotes	Snail
T5415	26283-26290	NN	denotes	protein
T5416	26291-26295	VBD	denotes	were
T5409	26296-26305	VBN	denotes	expressed
T5417	26305-26307	,	denotes	,
T5418	26307-26310	DT	denotes	the
T5419	26311-26318	NNS	denotes	effects
T5420	26319-26323	VBD	denotes	were
T5399	26324-26335	VBN	denotes	accentuated
T5421	26335-26336	.	denotes	.
T5422	26336-26528	sentence	denotes	In both back skin and ear skin, overall levels of E-cadherin and α-catenin were reduced, under conditions where β-catenin and Ajuba levels remained unchanged relative to controls (Figure 4B).
T5423	26337-26339	IN	denotes	In
T5425	26340-26344	CC	denotes	both
T5427	26345-26349	NN	denotes	back
T5426	26350-26354	NN	denotes	skin
T5428	26355-26358	CC	denotes	and
T5429	26359-26362	NN	denotes	ear
T5430	26363-26367	NN	denotes	skin
T5431	26367-26369	,	denotes	,
T5432	26369-26376	JJ	denotes	overall
T5433	26377-26383	NNS	denotes	levels
T5434	26384-26386	IN	denotes	of
T5435	26387-26388	NN	denotes	E
T5437	26388-26389	HYPH	denotes	-
T5436	26389-26397	NN	denotes	cadherin
T5438	26398-26401	CC	denotes	and
T5439	26402-26403	NN	denotes	α
T5441	26403-26404	HYPH	denotes	-
T5440	26404-26411	NN	denotes	catenin
T5442	26412-26416	VBD	denotes	were
T5424	26417-26424	VBN	denotes	reduced
T5443	26424-26426	,	denotes	,
T5444	26426-26431	IN	denotes	under
T5445	26432-26442	NNS	denotes	conditions
T5446	26443-26448	WRB	denotes	where
T5448	26449-26450	NN	denotes	β
T5450	26450-26451	HYPH	denotes	-
T5449	26451-26458	NN	denotes	catenin
T5452	26459-26462	CC	denotes	and
T5453	26463-26468	NN	denotes	Ajuba
T5451	26469-26475	NNS	denotes	levels
T5447	26476-26484	VBD	denotes	remained
T5454	26485-26494	JJ	denotes	unchanged
T5455	26495-26503	JJ	denotes	relative
T5456	26504-26506	IN	denotes	to
T5457	26507-26515	NNS	denotes	controls
T5458	26516-26517	-LRB-	denotes	(
T5460	26517-26523	NN	denotes	Figure
T5459	26524-26526	NN	denotes	4B
T5461	26526-26527	-RRB-	denotes	)
T5462	26527-26528	.	denotes	.
T5463	26528-26633	sentence	denotes	Taken together, these data were consistent with our results obtained from immunofluorescence microscopy.
T5464	26529-26534	VBN	denotes	Taken
T5466	26535-26543	RB	denotes	together
T5467	26543-26545	,	denotes	,
T5468	26545-26550	DT	denotes	these
T5469	26551-26555	NNS	denotes	data
T5465	26556-26560	VBD	denotes	were
T5470	26561-26571	JJ	denotes	consistent
T5471	26572-26576	IN	denotes	with
T5472	26577-26580	PRP$	denotes	our
T5473	26581-26588	NNS	denotes	results
T5474	26589-26597	VBN	denotes	obtained
T5475	26598-26602	IN	denotes	from
T5476	26603-26621	NN	denotes	immunofluorescence
T5477	26622-26632	NN	denotes	microscopy
T5478	26632-26633	.	denotes	.
T5479	26633-26829	sentence	denotes	A priori, the decrease in α-catenin levels could be due to either direct transcriptional repression by Snail or perturbations in AJ formation caused by the decrease in E-cadherin gene expression.
T5480	26634-26635	FW	denotes	A
T5481	26636-26642	FW	denotes	priori
T5483	26642-26644	,	denotes	,
T5484	26644-26647	DT	denotes	the
T5485	26648-26656	NN	denotes	decrease
T5486	26657-26659	IN	denotes	in
T5487	26660-26661	NN	denotes	α
T5489	26661-26662	HYPH	denotes	-
T5488	26662-26669	NN	denotes	catenin
T5490	26670-26676	NNS	denotes	levels
T5491	26677-26682	MD	denotes	could
T5482	26683-26685	VB	denotes	be
T5492	26686-26689	IN	denotes	due
T5493	26690-26692	IN	denotes	to
T5494	26693-26699	CC	denotes	either
T5496	26700-26706	JJ	denotes	direct
T5497	26707-26722	JJ	denotes	transcriptional
T5495	26723-26733	NN	denotes	repression
T5498	26734-26736	IN	denotes	by
T5499	26737-26742	NN	denotes	Snail
T5500	26743-26745	CC	denotes	or
T5501	26746-26759	NNS	denotes	perturbations
T5502	26760-26762	IN	denotes	in
T5503	26763-26765	NN	denotes	AJ
T5504	26766-26775	NN	denotes	formation
T5505	26776-26782	VBN	denotes	caused
T5506	26783-26785	IN	denotes	by
T5507	26786-26789	DT	denotes	the
T5508	26790-26798	NN	denotes	decrease
T5509	26799-26801	IN	denotes	in
T5510	26802-26803	NN	denotes	E
T5512	26803-26804	HYPH	denotes	-
T5511	26804-26812	NN	denotes	cadherin
T5514	26813-26817	NN	denotes	gene
T5513	26818-26828	NN	denotes	expression
T5515	26828-26829	.	denotes	.
T5516	26829-26999	sentence	denotes	To distinguish between these possibilities, we tested whether α-catenin levels could be restored by exogenous expression of E-cadherin in Snail-expressing keratinocytes.
T5517	26830-26832	TO	denotes	To
T5518	26833-26844	VB	denotes	distinguish
T5520	26845-26852	IN	denotes	between
T5521	26853-26858	DT	denotes	these
T5522	26859-26872	NNS	denotes	possibilities
T5523	26872-26874	,	denotes	,
T5524	26874-26876	PRP	denotes	we
T5519	26877-26883	VBD	denotes	tested
T5525	26884-26891	IN	denotes	whether
T5527	26892-26893	NN	denotes	α
T5529	26893-26894	HYPH	denotes	-
T5528	26894-26901	NN	denotes	catenin
T5530	26902-26908	NNS	denotes	levels
T5531	26909-26914	MD	denotes	could
T5532	26915-26917	VB	denotes	be
T5526	26918-26926	VBN	denotes	restored
T5533	26927-26929	IN	denotes	by
T5534	26930-26939	JJ	denotes	exogenous
T5535	26940-26950	NN	denotes	expression
T5536	26951-26953	IN	denotes	of
T5537	26954-26955	NN	denotes	E
T5539	26955-26956	HYPH	denotes	-
T5538	26956-26964	NN	denotes	cadherin
T5540	26965-26967	IN	denotes	in
T5541	26968-26973	NN	denotes	Snail
T5543	26973-26974	HYPH	denotes	-
T5542	26974-26984	VBG	denotes	expressing
T5544	26985-26998	NNS	denotes	keratinocytes
T5545	26998-26999	.	denotes	.
T5546	26999-27154	sentence	denotes	As shown in Figure 4C, transiently transfected keratinocytes expressing HA-tagged Snail displayed a loss of E-cadherin and α-catenin at cell-cell borders.
T5547	27000-27002	IN	denotes	As
T5548	27003-27008	VBN	denotes	shown
T5550	27009-27011	IN	denotes	in
T5551	27012-27018	NN	denotes	Figure
T5552	27019-27021	NN	denotes	4C
T5553	27021-27023	,	denotes	,
T5554	27023-27034	RB	denotes	transiently
T5555	27035-27046	VBN	denotes	transfected
T5556	27047-27060	NNS	denotes	keratinocytes
T5557	27061-27071	VBG	denotes	expressing
T5558	27072-27074	NN	denotes	HA
T5560	27074-27075	HYPH	denotes	-
T5559	27075-27081	VBN	denotes	tagged
T5561	27082-27087	NN	denotes	Snail
T5549	27088-27097	VBD	denotes	displayed
T5562	27098-27099	DT	denotes	a
T5563	27100-27104	NN	denotes	loss
T5564	27105-27107	IN	denotes	of
T5565	27108-27109	NN	denotes	E
T5567	27109-27110	HYPH	denotes	-
T5566	27110-27118	NN	denotes	cadherin
T5568	27119-27122	CC	denotes	and
T5569	27123-27124	NN	denotes	α
T5571	27124-27125	HYPH	denotes	-
T5570	27125-27132	NN	denotes	catenin
T5572	27133-27135	IN	denotes	at
T5573	27136-27140	NN	denotes	cell
T5575	27140-27141	HYPH	denotes	-
T5574	27141-27145	NN	denotes	cell
T5576	27146-27153	NNS	denotes	borders
T5577	27153-27154	.	denotes	.
T5578	27154-27344	sentence	denotes	Coexpression of exogenous HA-tagged E-cadherin not only enabled cell-cell border localization of E-cadherin protein, but also rescued the cell-cell border staining of α-catenin (Figure 4C).
T5579	27155-27167	NN	denotes	Coexpression
T5581	27168-27170	IN	denotes	of
T5582	27171-27180	JJ	denotes	exogenous
T5584	27181-27183	NN	denotes	HA
T5586	27183-27184	HYPH	denotes	-
T5585	27184-27190	VBN	denotes	tagged
T5587	27191-27192	NN	denotes	E
T5588	27192-27193	HYPH	denotes	-
T5583	27193-27201	NN	denotes	cadherin
T5589	27202-27205	RB	denotes	not
T5590	27206-27210	RB	denotes	only
T5580	27211-27218	VBD	denotes	enabled
T5591	27219-27223	NN	denotes	cell
T5593	27223-27224	HYPH	denotes	-
T5592	27224-27228	NN	denotes	cell
T5595	27229-27235	NN	denotes	border
T5594	27236-27248	NN	denotes	localization
T5596	27249-27251	IN	denotes	of
T5597	27252-27253	NN	denotes	E
T5599	27253-27254	HYPH	denotes	-
T5598	27254-27262	NN	denotes	cadherin
T5600	27263-27270	NN	denotes	protein
T5601	27270-27272	,	denotes	,
T5602	27272-27275	CC	denotes	but
T5603	27276-27280	RB	denotes	also
T5604	27281-27288	VBD	denotes	rescued
T5605	27289-27292	DT	denotes	the
T5607	27293-27297	NN	denotes	cell
T5609	27297-27298	HYPH	denotes	-
T5608	27298-27302	NN	denotes	cell
T5610	27303-27309	NN	denotes	border
T5606	27310-27318	NN	denotes	staining
T5611	27319-27321	IN	denotes	of
T5612	27322-27323	NN	denotes	α
T5614	27323-27324	HYPH	denotes	-
T5613	27324-27331	NN	denotes	catenin
T5615	27332-27333	-LRB-	denotes	(
T5617	27333-27339	NN	denotes	Figure
T5616	27340-27342	NN	denotes	4C
T5618	27342-27343	-RRB-	denotes	)
T5619	27343-27344	.	denotes	.
T5620	27344-27504	sentence	denotes	The ability to restore α-catenin expression and localization under these conditions argues against the notion that Snail transcriptionally represses α-catenin.
T5621	27345-27348	DT	denotes	The
T5622	27349-27356	NN	denotes	ability
T5624	27357-27359	TO	denotes	to
T5625	27360-27367	VB	denotes	restore
T5626	27368-27369	NN	denotes	α
T5628	27369-27370	HYPH	denotes	-
T5627	27370-27377	NN	denotes	catenin
T5629	27378-27388	NN	denotes	expression
T5630	27389-27392	CC	denotes	and
T5631	27393-27405	NN	denotes	localization
T5632	27406-27411	IN	denotes	under
T5633	27412-27417	DT	denotes	these
T5634	27418-27428	NNS	denotes	conditions
T5623	27429-27435	VBZ	denotes	argues
T5635	27436-27443	IN	denotes	against
T5636	27444-27447	DT	denotes	the
T5637	27448-27454	NN	denotes	notion
T5638	27455-27459	IN	denotes	that
T5640	27460-27465	NN	denotes	Snail
T5641	27466-27483	RB	denotes	transcriptionally
T5639	27484-27493	VBZ	denotes	represses
T5642	27494-27495	NN	denotes	α
T5644	27495-27496	HYPH	denotes	-
T5643	27496-27503	NN	denotes	catenin
T5645	27503-27504	.	denotes	.
T5646	27504-27635	sentence	denotes	Rather, the findings are consistent with a previous report that E-cadherin is required for the translation of α-catenin mRNA [22].
T5647	27505-27511	RB	denotes	Rather
T5649	27511-27513	,	denotes	,
T5650	27513-27516	DT	denotes	the
T5651	27517-27525	NNS	denotes	findings
T5648	27526-27529	VBP	denotes	are
T5652	27530-27540	JJ	denotes	consistent
T5653	27541-27545	IN	denotes	with
T5654	27546-27547	DT	denotes	a
T5656	27548-27556	JJ	denotes	previous
T5655	27557-27563	NN	denotes	report
T5657	27564-27568	IN	denotes	that
T5659	27569-27570	NN	denotes	E
T5661	27570-27571	HYPH	denotes	-
T5660	27571-27579	NN	denotes	cadherin
T5662	27580-27582	VBZ	denotes	is
T5658	27583-27591	VBN	denotes	required
T5663	27592-27595	IN	denotes	for
T5664	27596-27599	DT	denotes	the
T5665	27600-27611	NN	denotes	translation
T5666	27612-27614	IN	denotes	of
T5667	27615-27616	NN	denotes	α
T5669	27616-27617	HYPH	denotes	-
T5668	27617-27624	NN	denotes	catenin
T5670	27625-27629	NN	denotes	mRNA
T5671	27630-27631	-LRB-	denotes	[
T5672	27631-27633	CD	denotes	22
T5673	27633-27634	-RRB-	denotes	]
T5674	27634-27635	.	denotes	.
T5675	27635-27826	sentence	denotes	Despite the reductions in AJ markers, Tg skin still displayed sealed membranes and intercellular junctions that were largely intact, as judged by ultrastructural analyses (unpublished data).
T5676	27636-27643	IN	denotes	Despite
T5678	27644-27647	DT	denotes	the
T5679	27648-27658	NNS	denotes	reductions
T5680	27659-27661	IN	denotes	in
T5681	27662-27664	NN	denotes	AJ
T5682	27665-27672	NNS	denotes	markers
T5683	27672-27674	,	denotes	,
T5684	27674-27676	NN	denotes	Tg
T5685	27677-27681	NN	denotes	skin
T5686	27682-27687	RB	denotes	still
T5677	27688-27697	VBD	denotes	displayed
T5687	27698-27704	JJ	denotes	sealed
T5688	27705-27714	NNS	denotes	membranes
T5689	27715-27718	CC	denotes	and
T5690	27719-27732	JJ	denotes	intercellular
T5691	27733-27742	NNS	denotes	junctions
T5692	27743-27747	WDT	denotes	that
T5693	27748-27752	VBD	denotes	were
T5694	27753-27760	RB	denotes	largely
T5695	27761-27767	JJ	denotes	intact
T5696	27767-27769	,	denotes	,
T5697	27769-27771	IN	denotes	as
T5698	27772-27778	VBN	denotes	judged
T5699	27779-27781	IN	denotes	by
T5700	27782-27797	JJ	denotes	ultrastructural
T5701	27798-27806	NNS	denotes	analyses
T5702	27807-27808	-LRB-	denotes	(
T5704	27808-27819	JJ	denotes	unpublished
T5703	27820-27824	NNS	denotes	data
T5705	27824-27825	-RRB-	denotes	)
T5706	27825-27826	.	denotes	.
T5707	27826-28024	sentence	denotes	In this respect, the skin epithelium resembled that of the hair bud, where the down-regulation in junction proteins is permissive for cell-cell remodeling without abrogating intercellular adhesion.
T5708	27827-27829	IN	denotes	In
T5710	27830-27834	DT	denotes	this
T5711	27835-27842	NN	denotes	respect
T5712	27842-27844	,	denotes	,
T5713	27844-27847	DT	denotes	the
T5715	27848-27852	NN	denotes	skin
T5714	27853-27863	NN	denotes	epithelium
T5709	27864-27873	VBD	denotes	resembled
T5716	27874-27878	DT	denotes	that
T5717	27879-27881	IN	denotes	of
T5718	27882-27885	DT	denotes	the
T5720	27886-27890	NN	denotes	hair
T5719	27891-27894	NN	denotes	bud
T5721	27894-27896	,	denotes	,
T5722	27896-27901	WRB	denotes	where
T5724	27902-27905	DT	denotes	the
T5726	27906-27910	JJ	denotes	down
T5727	27910-27911	HYPH	denotes	-
T5725	27911-27921	NN	denotes	regulation
T5728	27922-27924	IN	denotes	in
T5729	27925-27933	NN	denotes	junction
T5730	27934-27942	NN	denotes	proteins
T5723	27943-27945	VBZ	denotes	is
T5731	27946-27956	JJ	denotes	permissive
T5732	27957-27960	IN	denotes	for
T5733	27961-27965	NN	denotes	cell
T5735	27965-27966	HYPH	denotes	-
T5734	27966-27970	NN	denotes	cell
T5736	27971-27981	NN	denotes	remodeling
T5737	27982-27989	IN	denotes	without
T5738	27990-28000	VBG	denotes	abrogating
T5739	28001-28014	JJ	denotes	intercellular
T5740	28015-28023	NN	denotes	adhesion
T5741	28023-28024	.	denotes	.
T5742	28024-28224	sentence	denotes	The similarities between Snail Tg epidermis and hair buds extended to the hyperproliferative state, leading us to wonder whether the down-regulation of AJ proteins might contribute to this condition.
T5743	28025-28028	DT	denotes	The
T5744	28029-28041	NNS	denotes	similarities
T5746	28042-28049	IN	denotes	between
T5747	28050-28055	NN	denotes	Snail
T5749	28056-28058	NN	denotes	Tg
T5748	28059-28068	NN	denotes	epidermis
T5750	28069-28072	CC	denotes	and
T5751	28073-28077	NN	denotes	hair
T5752	28078-28082	NNS	denotes	buds
T5745	28083-28091	VBD	denotes	extended
T5753	28092-28094	IN	denotes	to
T5754	28095-28098	DT	denotes	the
T5756	28099-28117	JJ	denotes	hyperproliferative
T5755	28118-28123	NN	denotes	state
T5757	28123-28125	,	denotes	,
T5758	28125-28132	VBG	denotes	leading
T5759	28133-28135	PRP	denotes	us
T5760	28136-28138	TO	denotes	to
T5761	28139-28145	VB	denotes	wonder
T5762	28146-28153	IN	denotes	whether
T5764	28154-28157	DT	denotes	the
T5766	28158-28162	JJ	denotes	down
T5767	28162-28163	HYPH	denotes	-
T5765	28163-28173	NN	denotes	regulation
T5768	28174-28176	IN	denotes	of
T5769	28177-28179	NN	denotes	AJ
T5770	28180-28188	NN	denotes	proteins
T5771	28189-28194	MD	denotes	might
T5763	28195-28205	VB	denotes	contribute
T5772	28206-28208	IN	denotes	to
T5773	28209-28213	DT	denotes	this
T5774	28214-28223	NN	denotes	condition
T5775	28223-28224	.	denotes	.
T5776	28224-28434	sentence	denotes	Given the increase in pMAPK staining in Snail Tg epidermis (see Figure 2G), we used pMAPK levels as our assay to test whether the loss of E-cadherin contributed to the Snail-mediated increase in proliferation.
T5777	28225-28230	VBN	denotes	Given
T5779	28231-28234	DT	denotes	the
T5780	28235-28243	NN	denotes	increase
T5781	28244-28246	IN	denotes	in
T5782	28247-28252	NN	denotes	pMAPK
T5783	28253-28261	NN	denotes	staining
T5784	28262-28264	IN	denotes	in
T5785	28265-28270	NN	denotes	Snail
T5787	28271-28273	NN	denotes	Tg
T5786	28274-28283	NN	denotes	epidermis
T5788	28284-28285	-LRB-	denotes	(
T5789	28285-28288	VB	denotes	see
T5790	28289-28295	NN	denotes	Figure
T5791	28296-28298	NN	denotes	2G
T5792	28298-28299	-RRB-	denotes	)
T5793	28299-28301	,	denotes	,
T5794	28301-28303	PRP	denotes	we
T5778	28304-28308	VBD	denotes	used
T5795	28309-28314	NN	denotes	pMAPK
T5796	28315-28321	NNS	denotes	levels
T5797	28322-28324	IN	denotes	as
T5798	28325-28328	PRP$	denotes	our
T5799	28329-28334	NN	denotes	assay
T5800	28335-28337	TO	denotes	to
T5801	28338-28342	VB	denotes	test
T5802	28343-28350	IN	denotes	whether
T5804	28351-28354	DT	denotes	the
T5805	28355-28359	NN	denotes	loss
T5806	28360-28362	IN	denotes	of
T5807	28363-28364	NN	denotes	E
T5809	28364-28365	HYPH	denotes	-
T5808	28365-28373	NN	denotes	cadherin
T5803	28374-28385	VBD	denotes	contributed
T5810	28386-28388	IN	denotes	to
T5811	28389-28392	DT	denotes	the
T5813	28393-28398	NN	denotes	Snail
T5815	28398-28399	HYPH	denotes	-
T5814	28399-28407	VBN	denotes	mediated
T5812	28408-28416	NN	denotes	increase
T5816	28417-28419	IN	denotes	in
T5817	28420-28433	NN	denotes	proliferation
T5818	28433-28434	.	denotes	.
T5819	28434-28607	sentence	denotes	Consistent with our in vivo observations, transfected keratinocytes expressing Snail exhibited a substantial increase in pMAPK levels relative to control cells (Figure 4D).
T5820	28435-28445	JJ	denotes	Consistent
T5822	28446-28450	IN	denotes	with
T5823	28451-28454	PRP$	denotes	our
T5825	28455-28457	FW	denotes	in
T5826	28458-28462	FW	denotes	vivo
T5824	28463-28475	NNS	denotes	observations
T5827	28475-28477	,	denotes	,
T5828	28477-28488	VBN	denotes	transfected
T5829	28489-28502	NNS	denotes	keratinocytes
T5830	28503-28513	VBG	denotes	expressing
T5831	28514-28519	NN	denotes	Snail
T5821	28520-28529	VBD	denotes	exhibited
T5832	28530-28531	DT	denotes	a
T5834	28532-28543	JJ	denotes	substantial
T5833	28544-28552	NN	denotes	increase
T5835	28553-28555	IN	denotes	in
T5836	28556-28561	NN	denotes	pMAPK
T5837	28562-28568	NNS	denotes	levels
T5838	28569-28577	JJ	denotes	relative
T5839	28578-28580	IN	denotes	to
T5840	28581-28588	NN	denotes	control
T5841	28589-28594	NNS	denotes	cells
T5842	28595-28596	-LRB-	denotes	(
T5844	28596-28602	NN	denotes	Figure
T5843	28603-28605	NN	denotes	4D
T5845	28605-28606	-RRB-	denotes	)
T5846	28606-28607	.	denotes	.
T5847	28607-28679	sentence	denotes	Coexpression of E-cadherin with Snail appeared to abrogate this effect.
T5848	28608-28620	NN	denotes	Coexpression
T5850	28621-28623	IN	denotes	of
T5851	28624-28625	NN	denotes	E
T5853	28625-28626	HYPH	denotes	-
T5852	28626-28634	NN	denotes	cadherin
T5854	28635-28639	IN	denotes	with
T5855	28640-28645	NN	denotes	Snail
T5849	28646-28654	VBD	denotes	appeared
T5856	28655-28657	TO	denotes	to
T5857	28658-28666	VB	denotes	abrogate
T5858	28667-28671	DT	denotes	this
T5859	28672-28678	NN	denotes	effect
T5860	28678-28679	.	denotes	.
T5861	28679-28890	sentence	denotes	Together, these findings raised the possibility that an AJ-associated protein that is normally sequestered at the plasma membrane may participate in a proliferation signaling pathway when AJs are deconstructed.
T5862	28680-28688	RB	denotes	Together
T5864	28688-28690	,	denotes	,
T5865	28690-28695	DT	denotes	these
T5866	28696-28704	NNS	denotes	findings
T5863	28705-28711	VBD	denotes	raised
T5867	28712-28715	DT	denotes	the
T5868	28716-28727	NN	denotes	possibility
T5869	28728-28732	IN	denotes	that
T5871	28733-28735	DT	denotes	an
T5873	28736-28738	NN	denotes	AJ
T5875	28738-28739	HYPH	denotes	-
T5874	28739-28749	VBN	denotes	associated
T5872	28750-28757	NN	denotes	protein
T5876	28758-28762	WDT	denotes	that
T5878	28763-28765	VBZ	denotes	is
T5879	28766-28774	RB	denotes	normally
T5877	28775-28786	VBN	denotes	sequestered
T5880	28787-28789	IN	denotes	at
T5881	28790-28793	DT	denotes	the
T5883	28794-28800	NN	denotes	plasma
T5882	28801-28809	NN	denotes	membrane
T5884	28810-28813	MD	denotes	may
T5870	28814-28825	VB	denotes	participate
T5885	28826-28828	IN	denotes	in
T5886	28829-28830	DT	denotes	a
T5888	28831-28844	NN	denotes	proliferation
T5889	28845-28854	NN	denotes	signaling
T5887	28855-28862	NN	denotes	pathway
T5890	28863-28867	WRB	denotes	when
T5892	28868-28871	NNS	denotes	AJs
T5893	28872-28875	VBP	denotes	are
T5891	28876-28889	VBN	denotes	deconstructed
T5894	28889-28890	.	denotes	.
T5895	28890-29130	sentence	denotes	Numerous studies have correlated a down-regulation of E-cadherin with a translocation of β-catenin to the nucleus and a transactivation of genes that are regulated by the LEF-1/T cell factor (TCF) family of DNA binding proteins [23,24,25].
T5896	28891-28899	JJ	denotes	Numerous
T5897	28900-28907	NNS	denotes	studies
T5899	28908-28912	VBP	denotes	have
T5898	28913-28923	VBN	denotes	correlated
T5900	28924-28925	DT	denotes	a
T5902	28926-28930	JJ	denotes	down
T5903	28930-28931	HYPH	denotes	-
T5901	28931-28941	NN	denotes	regulation
T5904	28942-28944	IN	denotes	of
T5905	28945-28946	NN	denotes	E
T5907	28946-28947	HYPH	denotes	-
T5906	28947-28955	NN	denotes	cadherin
T5908	28956-28960	IN	denotes	with
T5909	28961-28962	DT	denotes	a
T5910	28963-28976	NN	denotes	translocation
T5911	28977-28979	IN	denotes	of
T5912	28980-28981	NN	denotes	β
T5914	28981-28982	HYPH	denotes	-
T5913	28982-28989	NN	denotes	catenin
T5915	28990-28992	IN	denotes	to
T5916	28993-28996	DT	denotes	the
T5917	28997-29004	NN	denotes	nucleus
T5918	29005-29008	CC	denotes	and
T5919	29009-29010	DT	denotes	a
T5920	29011-29026	NN	denotes	transactivation
T5921	29027-29029	IN	denotes	of
T5922	29030-29035	NNS	denotes	genes
T5923	29036-29040	WDT	denotes	that
T5925	29041-29044	VBP	denotes	are
T5924	29045-29054	VBN	denotes	regulated
T5926	29055-29057	IN	denotes	by
T5927	29058-29061	DT	denotes	the
T5929	29062-29065	NN	denotes	LEF
T5930	29065-29066	HYPH	denotes	-
T5931	29066-29067	CD	denotes	1
T5932	29067-29068	HYPH	denotes	/
T5933	29068-29069	NN	denotes	T
T5934	29070-29074	NN	denotes	cell
T5935	29075-29081	NN	denotes	factor
T5936	29082-29083	-LRB-	denotes	(
T5937	29083-29086	NN	denotes	TCF
T5938	29086-29087	-RRB-	denotes	)
T5928	29088-29094	NN	denotes	family
T5939	29095-29097	IN	denotes	of
T5940	29098-29101	NN	denotes	DNA
T5941	29102-29109	VBG	denotes	binding
T5942	29110-29118	NN	denotes	proteins
T5943	29119-29120	-LRB-	denotes	[
T5945	29120-29122	CD	denotes	23
T5946	29122-29123	,	denotes	,
T5947	29123-29125	CD	denotes	24
T5948	29125-29126	,	denotes	,
T5944	29126-29128	CD	denotes	25
T5949	29128-29129	-RRB-	denotes	]
T5950	29129-29130	.	denotes	.
T5951	29130-29336	sentence	denotes	The presence of nuclear cyclin D in hyperproliferative Snail Tg epidermis was particularly intriguing since prior studies have reported cyclin D gene as a direct target of TCF/β-catenin transcription [26].
T5952	29131-29134	DT	denotes	The
T5953	29135-29143	NN	denotes	presence
T5955	29144-29146	IN	denotes	of
T5956	29147-29154	JJ	denotes	nuclear
T5958	29155-29161	NN	denotes	cyclin
T5957	29162-29163	NN	denotes	D
T5959	29164-29166	IN	denotes	in
T5960	29167-29185	JJ	denotes	hyperproliferative
T5962	29186-29191	NN	denotes	Snail
T5963	29192-29194	NN	denotes	Tg
T5961	29195-29204	NN	denotes	epidermis
T5954	29205-29208	VBD	denotes	was
T5964	29209-29221	RB	denotes	particularly
T5965	29222-29232	JJ	denotes	intriguing
T5966	29233-29238	IN	denotes	since
T5968	29239-29244	JJ	denotes	prior
T5969	29245-29252	NNS	denotes	studies
T5970	29253-29257	VBP	denotes	have
T5967	29258-29266	VBN	denotes	reported
T5971	29267-29273	NN	denotes	cyclin
T5972	29274-29275	NN	denotes	D
T5973	29276-29280	NN	denotes	gene
T5974	29281-29283	IN	denotes	as
T5975	29284-29285	DT	denotes	a
T5977	29286-29292	JJ	denotes	direct
T5976	29293-29299	NN	denotes	target
T5978	29300-29302	IN	denotes	of
T5979	29303-29306	NN	denotes	TCF
T5981	29306-29307	HYPH	denotes	/
T5980	29307-29308	NN	denotes	β
T5983	29308-29309	HYPH	denotes	-
T5984	29309-29316	NN	denotes	catenin
T5982	29317-29330	NN	denotes	transcription
T5985	29331-29332	-LRB-	denotes	[
T5986	29332-29334	CD	denotes	26
T5987	29334-29335	-RRB-	denotes	]
T5988	29335-29336	.	denotes	.
T5989	29336-29546	sentence	denotes	This said, we did not detect nuclear β-catenin in our Tg epidermis, and mating the Snail Tg mice against the TOPGal reporter mouse [20] gave no signs of ectopic LEF-1/Tcf/β-catenin activity (unpublished data).
T5990	29337-29341	DT	denotes	This
T5991	29342-29346	VBN	denotes	said
T5993	29346-29348	,	denotes	,
T5994	29348-29350	PRP	denotes	we
T5995	29351-29354	VBD	denotes	did
T5996	29355-29358	RB	denotes	not
T5992	29359-29365	VB	denotes	detect
T5997	29366-29373	JJ	denotes	nuclear
T5999	29374-29375	NN	denotes	β
T6000	29375-29376	HYPH	denotes	-
T5998	29376-29383	NN	denotes	catenin
T6001	29384-29386	IN	denotes	in
T6002	29387-29390	PRP$	denotes	our
T6004	29391-29393	NN	denotes	Tg
T6003	29394-29403	NN	denotes	epidermis
T6005	29403-29405	,	denotes	,
T6006	29405-29408	CC	denotes	and
T6007	29409-29415	VBG	denotes	mating
T6009	29416-29419	DT	denotes	the
T6011	29420-29425	NN	denotes	Snail
T6012	29426-29428	NN	denotes	Tg
T6010	29429-29433	NNS	denotes	mice
T6013	29434-29441	IN	denotes	against
T6014	29442-29445	DT	denotes	the
T6016	29446-29452	NN	denotes	TOPGal
T6017	29453-29461	NN	denotes	reporter
T6015	29462-29467	NN	denotes	mouse
T6018	29468-29469	-LRB-	denotes	[
T6019	29469-29471	CD	denotes	20
T6020	29471-29472	-RRB-	denotes	]
T6008	29473-29477	VBD	denotes	gave
T6021	29478-29480	DT	denotes	no
T6022	29481-29486	NNS	denotes	signs
T6023	29487-29489	IN	denotes	of
T6024	29490-29497	JJ	denotes	ectopic
T6026	29498-29501	NN	denotes	LEF
T6027	29501-29502	HYPH	denotes	-
T6028	29502-29503	CD	denotes	1
T6029	29503-29504	HYPH	denotes	/
T6030	29504-29507	NN	denotes	Tcf
T6031	29507-29508	HYPH	denotes	/
T6032	29508-29509	NN	denotes	β
T6034	29509-29510	HYPH	denotes	-
T6033	29510-29517	NN	denotes	catenin
T6025	29518-29526	NN	denotes	activity
T6035	29527-29528	-LRB-	denotes	(
T6037	29528-29539	JJ	denotes	unpublished
T6036	29540-29544	NNS	denotes	data
T6038	29544-29545	-RRB-	denotes	)
T6039	29545-29546	.	denotes	.
T6040	29546-29687	sentence	denotes	We next turned to the presence of cytoplasmic Ajuba for a possible mechanistic link to the proliferative increase in our Snail Tg epidermis.
T6041	29547-29549	PRP	denotes	We
T6043	29550-29554	RB	denotes	next
T6042	29555-29561	VBD	denotes	turned
T6044	29562-29564	IN	denotes	to
T6045	29565-29568	DT	denotes	the
T6046	29569-29577	NN	denotes	presence
T6047	29578-29580	IN	denotes	of
T6048	29581-29592	JJ	denotes	cytoplasmic
T6049	29593-29598	NN	denotes	Ajuba
T6050	29599-29602	IN	denotes	for
T6051	29603-29604	DT	denotes	a
T6053	29605-29613	JJ	denotes	possible
T6054	29614-29625	JJ	denotes	mechanistic
T6052	29626-29630	NN	denotes	link
T6055	29631-29633	IN	denotes	to
T6056	29634-29637	DT	denotes	the
T6058	29638-29651	JJ	denotes	proliferative
T6057	29652-29660	NN	denotes	increase
T6059	29661-29663	IN	denotes	in
T6060	29664-29667	PRP$	denotes	our
T6062	29668-29673	NN	denotes	Snail
T6063	29674-29676	NN	denotes	Tg
T6061	29677-29686	NN	denotes	epidermis
T6064	29686-29687	.	denotes	.
T6065	29687-29937	sentence	denotes	In addition to its documented ability to bind α-catenin [10], Ajuba can also associate with growth factor receptor-bound protein-2 (Grb-2)/son of sevenless (Sos), the nucleotide exchange factor for Ras, which is upstream from activation of MAPK [9].
T6066	29688-29690	IN	denotes	In
T6068	29691-29699	NN	denotes	addition
T6069	29700-29702	IN	denotes	to
T6070	29703-29706	PRP$	denotes	its
T6072	29707-29717	VBN	denotes	documented
T6071	29718-29725	NN	denotes	ability
T6073	29726-29728	TO	denotes	to
T6074	29729-29733	VB	denotes	bind
T6075	29734-29735	NN	denotes	α
T6077	29735-29736	HYPH	denotes	-
T6076	29736-29743	NN	denotes	catenin
T6078	29744-29745	-LRB-	denotes	[
T6079	29745-29747	CD	denotes	10
T6080	29747-29748	-RRB-	denotes	]
T6081	29748-29750	,	denotes	,
T6082	29750-29755	NN	denotes	Ajuba
T6083	29756-29759	MD	denotes	can
T6084	29760-29764	RB	denotes	also
T6067	29765-29774	VB	denotes	associate
T6085	29775-29779	IN	denotes	with
T6086	29780-29786	NN	denotes	growth
T6087	29787-29793	NN	denotes	factor
T6088	29794-29802	NN	denotes	receptor
T6090	29802-29803	HYPH	denotes	-
T6089	29803-29808	JJ	denotes	bound
T6091	29809-29816	NN	denotes	protein
T6092	29816-29817	HYPH	denotes	-
T6093	29817-29818	CD	denotes	2
T6094	29819-29820	-LRB-	denotes	(
T6095	29820-29823	NN	denotes	Grb
T6096	29823-29824	HYPH	denotes	-
T6097	29824-29825	CD	denotes	2
T6098	29825-29826	-RRB-	denotes	)
T6099	29826-29827	HYPH	denotes	/
T6100	29827-29830	NN	denotes	son
T6101	29831-29833	IN	denotes	of
T6102	29834-29843	NN	denotes	sevenless
T6103	29844-29845	-LRB-	denotes	(
T6104	29845-29848	NN	denotes	Sos
T6105	29848-29849	-RRB-	denotes	)
T6106	29849-29851	,	denotes	,
T6107	29851-29854	DT	denotes	the
T6109	29855-29865	NN	denotes	nucleotide
T6110	29866-29874	NN	denotes	exchange
T6108	29875-29881	NN	denotes	factor
T6111	29882-29885	IN	denotes	for
T6112	29886-29889	NN	denotes	Ras
T6113	29889-29891	,	denotes	,
T6114	29891-29896	WDT	denotes	which
T6115	29897-29899	VBZ	denotes	is
T6116	29900-29908	RB	denotes	upstream
T6117	29909-29913	IN	denotes	from
T6118	29914-29924	NN	denotes	activation
T6119	29925-29927	IN	denotes	of
T6120	29928-29932	NN	denotes	MAPK
T6121	29933-29934	-LRB-	denotes	[
T6122	29934-29935	CD	denotes	9
T6123	29935-29936	-RRB-	denotes	]
T6124	29936-29937	.	denotes	.
T6125	29937-30095	sentence	denotes	Given the increase in pMAPK staining in Tg skin, we examined the possibility that Ajuba might have changed its binding partner in Snail-expressing epidermis.
T6126	29938-29943	VBN	denotes	Given
T6128	29944-29947	DT	denotes	the
T6129	29948-29956	NN	denotes	increase
T6130	29957-29959	IN	denotes	in
T6131	29960-29965	NN	denotes	pMAPK
T6132	29966-29974	NN	denotes	staining
T6133	29975-29977	IN	denotes	in
T6134	29978-29980	NN	denotes	Tg
T6135	29981-29985	NN	denotes	skin
T6136	29985-29987	,	denotes	,
T6137	29987-29989	PRP	denotes	we
T6127	29990-29998	VBD	denotes	examined
T6138	29999-30002	DT	denotes	the
T6139	30003-30014	NN	denotes	possibility
T6140	30015-30019	IN	denotes	that
T6142	30020-30025	NN	denotes	Ajuba
T6143	30026-30031	MD	denotes	might
T6144	30032-30036	VB	denotes	have
T6141	30037-30044	VBN	denotes	changed
T6145	30045-30048	PRP$	denotes	its
T6147	30049-30056	NN	denotes	binding
T6146	30057-30064	NN	denotes	partner
T6148	30065-30067	IN	denotes	in
T6149	30068-30073	NN	denotes	Snail
T6151	30073-30074	HYPH	denotes	-
T6150	30074-30084	VBG	denotes	expressing
T6152	30085-30094	NN	denotes	epidermis
T6153	30094-30095	.	denotes	.
T6154	30095-30284	sentence	denotes	Interestingly, Ajuba was readily detected in anti-Grb-2 immunoprecipitates of protein lysates from skins of Snail Tg mice but not from the corresponding wild-type (WT) animals (Figure 4E).
T6155	30096-30109	RB	denotes	Interestingly
T6157	30109-30111	,	denotes	,
T6158	30111-30116	NN	denotes	Ajuba
T6159	30117-30120	VBD	denotes	was
T6160	30121-30128	RB	denotes	readily
T6156	30129-30137	VBN	denotes	detected
T6161	30138-30140	IN	denotes	in
T6162	30141-30149	JJ	denotes	anti-Grb
T6164	30149-30150	HYPH	denotes	-
T6165	30150-30151	CD	denotes	2
T6163	30152-30170	NNS	denotes	immunoprecipitates
T6166	30171-30173	IN	denotes	of
T6167	30174-30181	NN	denotes	protein
T6168	30182-30189	NNS	denotes	lysates
T6169	30190-30194	IN	denotes	from
T6170	30195-30200	NNS	denotes	skins
T6171	30201-30203	IN	denotes	of
T6172	30204-30209	NN	denotes	Snail
T6174	30210-30212	NN	denotes	Tg
T6173	30213-30217	NNS	denotes	mice
T6175	30218-30221	CC	denotes	but
T6176	30222-30225	RB	denotes	not
T6177	30226-30230	IN	denotes	from
T6178	30231-30234	DT	denotes	the
T6180	30235-30248	JJ	denotes	corresponding
T6181	30249-30253	JJ	denotes	wild
T6183	30253-30254	HYPH	denotes	-
T6182	30254-30258	NN	denotes	type
T6184	30259-30260	-LRB-	denotes	(
T6185	30260-30262	NN	denotes	WT
T6186	30262-30263	-RRB-	denotes	)
T6179	30264-30271	NNS	denotes	animals
T6187	30272-30273	-LRB-	denotes	(
T6189	30273-30279	NN	denotes	Figure
T6188	30280-30282	NN	denotes	4E
T6190	30282-30283	-RRB-	denotes	)
T6191	30283-30284	.	denotes	.
T6192	30284-30511	sentence	denotes	When these experiments were repeated with α-catenin-null epidermis, a similar Grb-2-Ajuba association was detected, and again, this interaction was not detected in the protein extracts from control littermate skin (Figure 4E).
T6193	30285-30289	WRB	denotes	When
T6195	30290-30295	DT	denotes	these
T6196	30296-30307	NNS	denotes	experiments
T6197	30308-30312	VBD	denotes	were
T6194	30313-30321	VBN	denotes	repeated
T6199	30322-30326	IN	denotes	with
T6200	30327-30328	NN	denotes	α
T6202	30328-30329	HYPH	denotes	-
T6201	30329-30336	NN	denotes	catenin
T6204	30336-30337	HYPH	denotes	-
T6203	30337-30341	JJ	denotes	null
T6205	30342-30351	NN	denotes	epidermis
T6206	30351-30353	,	denotes	,
T6207	30353-30354	DT	denotes	a
T6209	30355-30362	JJ	denotes	similar
T6210	30363-30366	NN	denotes	Grb
T6212	30366-30367	HYPH	denotes	-
T6213	30367-30368	CD	denotes	2
T6214	30368-30369	HYPH	denotes	-
T6211	30369-30374	NN	denotes	Ajuba
T6208	30375-30386	NN	denotes	association
T6215	30387-30390	VBD	denotes	was
T6198	30391-30399	VBN	denotes	detected
T6216	30399-30401	,	denotes	,
T6217	30401-30404	CC	denotes	and
T6218	30405-30410	RB	denotes	again
T6220	30410-30412	,	denotes	,
T6221	30412-30416	DT	denotes	this
T6222	30417-30428	NN	denotes	interaction
T6223	30429-30432	VBD	denotes	was
T6224	30433-30436	RB	denotes	not
T6219	30437-30445	VBN	denotes	detected
T6225	30446-30448	IN	denotes	in
T6226	30449-30452	DT	denotes	the
T6228	30453-30460	NN	denotes	protein
T6227	30461-30469	NNS	denotes	extracts
T6229	30470-30474	IN	denotes	from
T6230	30475-30482	NN	denotes	control
T6232	30483-30493	NN	denotes	littermate
T6231	30494-30498	NN	denotes	skin
T6233	30499-30500	-LRB-	denotes	(
T6235	30500-30506	NN	denotes	Figure
T6234	30507-30509	NN	denotes	4E
T6236	30509-30510	-RRB-	denotes	)
T6237	30510-30511	.	denotes	.
T6238	30511-30719	sentence	denotes	Together, these data demonstrate that the reduction in α-catenin levels, either by Snail-mediated down-regulation of E-cadherin or by α-catenin conditional targeting, allows Ajuba to interact with Grb-2/Sos.
T6239	30512-30520	RB	denotes	Together
T6241	30520-30522	,	denotes	,
T6242	30522-30527	DT	denotes	these
T6243	30528-30532	NNS	denotes	data
T6240	30533-30544	VBP	denotes	demonstrate
T6244	30545-30549	IN	denotes	that
T6246	30550-30553	DT	denotes	the
T6247	30554-30563	NN	denotes	reduction
T6248	30564-30566	IN	denotes	in
T6249	30567-30568	NN	denotes	α
T6251	30568-30569	HYPH	denotes	-
T6250	30569-30576	NN	denotes	catenin
T6252	30577-30583	NNS	denotes	levels
T6253	30583-30585	,	denotes	,
T6254	30585-30591	CC	denotes	either
T6255	30592-30594	IN	denotes	by
T6256	30595-30600	NN	denotes	Snail
T6258	30600-30601	HYPH	denotes	-
T6257	30601-30609	VBN	denotes	mediated
T6260	30610-30614	JJ	denotes	down
T6261	30614-30615	HYPH	denotes	-
T6259	30615-30625	NN	denotes	regulation
T6262	30626-30628	IN	denotes	of
T6263	30629-30630	NN	denotes	E
T6265	30630-30631	HYPH	denotes	-
T6264	30631-30639	NN	denotes	cadherin
T6266	30640-30642	CC	denotes	or
T6267	30643-30645	IN	denotes	by
T6268	30646-30647	NN	denotes	α
T6270	30647-30648	HYPH	denotes	-
T6269	30648-30655	NN	denotes	catenin
T6271	30656-30667	JJ	denotes	conditional
T6272	30668-30677	NN	denotes	targeting
T6273	30677-30679	,	denotes	,
T6245	30679-30685	VBZ	denotes	allows
T6274	30686-30691	NN	denotes	Ajuba
T6276	30692-30694	TO	denotes	to
T6275	30695-30703	VB	denotes	interact
T6277	30704-30708	IN	denotes	with
T6278	30709-30712	NN	denotes	Grb
T6279	30712-30713	HYPH	denotes	-
T6280	30713-30714	CD	denotes	2
T6281	30714-30715	HYPH	denotes	/
T6282	30715-30718	NN	denotes	Sos
T6283	30718-30719	.	denotes	.
T6284	30719-30997	sentence	denotes	If the competition between Grb-2/Sos and α-catenin for Ajuba is functionally relevant to the hyperproliferative state of a keratinocyte, then overexpression of Ajuba would be expected to bypass the competition and promote activation of the Ras-MAPK pathway in WT keratinocytes.
T6285	30720-30722	IN	denotes	If
T6287	30723-30726	DT	denotes	the
T6288	30727-30738	NN	denotes	competition
T6289	30739-30746	IN	denotes	between
T6290	30747-30750	NN	denotes	Grb
T6291	30750-30751	HYPH	denotes	-
T6292	30751-30752	CD	denotes	2
T6293	30752-30753	HYPH	denotes	/
T6294	30753-30756	NN	denotes	Sos
T6295	30757-30760	CC	denotes	and
T6296	30761-30762	NN	denotes	α
T6298	30762-30763	HYPH	denotes	-
T6297	30763-30770	NN	denotes	catenin
T6299	30771-30774	IN	denotes	for
T6300	30775-30780	NN	denotes	Ajuba
T6286	30781-30783	VBZ	denotes	is
T6302	30784-30796	RB	denotes	functionally
T6303	30797-30805	JJ	denotes	relevant
T6304	30806-30808	IN	denotes	to
T6305	30809-30812	DT	denotes	the
T6307	30813-30831	JJ	denotes	hyperproliferative
T6306	30832-30837	NN	denotes	state
T6308	30838-30840	IN	denotes	of
T6309	30841-30842	DT	denotes	a
T6310	30843-30855	NN	denotes	keratinocyte
T6311	30855-30857	,	denotes	,
T6312	30857-30861	RB	denotes	then
T6313	30862-30876	NN	denotes	overexpression
T6314	30877-30879	IN	denotes	of
T6315	30880-30885	NN	denotes	Ajuba
T6316	30886-30891	MD	denotes	would
T6317	30892-30894	VB	denotes	be
T6301	30895-30903	VBN	denotes	expected
T6318	30904-30906	TO	denotes	to
T6319	30907-30913	VB	denotes	bypass
T6320	30914-30917	DT	denotes	the
T6321	30918-30929	NN	denotes	competition
T6322	30930-30933	CC	denotes	and
T6323	30934-30941	VB	denotes	promote
T6324	30942-30952	NN	denotes	activation
T6325	30953-30955	IN	denotes	of
T6326	30956-30959	DT	denotes	the
T6328	30960-30963	NN	denotes	Ras
T6330	30963-30964	HYPH	denotes	-
T6329	30964-30968	NN	denotes	MAPK
T6327	30969-30976	NN	denotes	pathway
T6331	30977-30979	IN	denotes	in
T6332	30980-30982	NN	denotes	WT
T6333	30983-30996	NNS	denotes	keratinocytes
T6334	30996-30997	.	denotes	.
T6335	30997-31228	sentence	denotes	Indeed, when serum-starved keratinocytes were transiently transfected with an Ajuba expression vector, the levels of pMAPK were not only elevated but also comparable to those transfected with the K14-HASnail transgene (Figure 4F).
T6336	30998-31004	RB	denotes	Indeed
T6338	31004-31006	,	denotes	,
T6339	31006-31010	WRB	denotes	when
T6341	31011-31016	NN	denotes	serum
T6343	31016-31017	HYPH	denotes	-
T6342	31017-31024	VBN	denotes	starved
T6344	31025-31038	NNS	denotes	keratinocytes
T6345	31039-31043	VBD	denotes	were
T6346	31044-31055	RB	denotes	transiently
T6340	31056-31067	VBN	denotes	transfected
T6347	31068-31072	IN	denotes	with
T6348	31073-31075	DT	denotes	an
T6350	31076-31081	NN	denotes	Ajuba
T6351	31082-31092	NN	denotes	expression
T6349	31093-31099	NN	denotes	vector
T6352	31099-31101	,	denotes	,
T6353	31101-31104	DT	denotes	the
T6354	31105-31111	NNS	denotes	levels
T6355	31112-31114	IN	denotes	of
T6356	31115-31120	NN	denotes	pMAPK
T6337	31121-31125	VBD	denotes	were
T6357	31126-31129	RB	denotes	not
T6359	31130-31134	RB	denotes	only
T6358	31135-31143	JJ	denotes	elevated
T6360	31144-31147	CC	denotes	but
T6361	31148-31152	RB	denotes	also
T6362	31153-31163	JJ	denotes	comparable
T6363	31164-31166	IN	denotes	to
T6364	31167-31172	DT	denotes	those
T6365	31173-31184	VBN	denotes	transfected
T6366	31185-31189	IN	denotes	with
T6367	31190-31193	DT	denotes	the
T6369	31194-31197	NN	denotes	K14
T6371	31197-31198	HYPH	denotes	-
T6370	31198-31205	NN	denotes	HASnail
T6368	31206-31215	NN	denotes	transgene
T6372	31216-31217	-LRB-	denotes	(
T6374	31217-31223	NN	denotes	Figure
T6373	31224-31226	NN	denotes	4F
T6375	31226-31227	-RRB-	denotes	)
T6376	31227-31228	.	denotes	.
T6377	31228-31410	sentence	denotes	This activation was abolished when cells were treated with a small peptide inhibitor that specifically interrupts the Grb-2/Sos interaction (Figure 4F; see lanes marked “inh”) [27].
T6378	31229-31233	DT	denotes	This
T6379	31234-31244	NN	denotes	activation
T6381	31245-31248	VBD	denotes	was
T6380	31249-31258	VBN	denotes	abolished
T6382	31259-31263	WRB	denotes	when
T6384	31264-31269	NNS	denotes	cells
T6385	31270-31274	VBD	denotes	were
T6383	31275-31282	VBN	denotes	treated
T6386	31283-31287	IN	denotes	with
T6387	31288-31289	DT	denotes	a
T6389	31290-31295	JJ	denotes	small
T6390	31296-31303	NN	denotes	peptide
T6388	31304-31313	NN	denotes	inhibitor
T6391	31314-31318	WDT	denotes	that
T6393	31319-31331	RB	denotes	specifically
T6392	31332-31342	VBZ	denotes	interrupts
T6394	31343-31346	DT	denotes	the
T6396	31347-31350	NN	denotes	Grb
T6397	31350-31351	HYPH	denotes	-
T6398	31351-31352	CD	denotes	2
T6399	31352-31353	HYPH	denotes	/
T6400	31353-31356	NN	denotes	Sos
T6395	31357-31368	NN	denotes	interaction
T6401	31369-31370	-LRB-	denotes	(
T6403	31370-31376	NN	denotes	Figure
T6402	31377-31379	NN	denotes	4F
T6404	31379-31380	:	denotes	;
T6405	31381-31384	VB	denotes	see
T6406	31385-31390	NNS	denotes	lanes
T6407	31391-31397	VBN	denotes	marked
T6408	31398-31399	``	denotes	“
T6409	31399-31402	NN	denotes	inh
T6410	31402-31403	''	denotes	”
T6411	31403-31404	-RRB-	denotes	)
T6412	31405-31406	-LRB-	denotes	[
T6413	31406-31408	CD	denotes	27
T6414	31408-31409	-RRB-	denotes	]
T6415	31409-31410	.	denotes	.
T6416	31410-31504	sentence	denotes	Ajuba's pre-LIM domain is the segment that associates with Grb-2's Src-homology 3 domain [9].
T6417	31411-31416	NN	denotes	Ajuba
T6419	31416-31418	POS	denotes	's
T6420	31419-31426	JJ	denotes	pre-LIM
T6418	31427-31433	NN	denotes	domain
T6421	31434-31436	VBZ	denotes	is
T6422	31437-31440	DT	denotes	the
T6423	31441-31448	NN	denotes	segment
T6424	31449-31453	WDT	denotes	that
T6425	31454-31464	VBZ	denotes	associates
T6426	31465-31469	IN	denotes	with
T6427	31470-31473	NN	denotes	Grb
T6429	31473-31474	HYPH	denotes	-
T6430	31474-31475	CD	denotes	2
T6431	31475-31477	POS	denotes	's
T6432	31478-31481	NN	denotes	Src
T6434	31481-31482	HYPH	denotes	-
T6433	31482-31490	NN	denotes	homology
T6435	31491-31492	CD	denotes	3
T6428	31493-31499	NN	denotes	domain
T6436	31500-31501	-LRB-	denotes	[
T6437	31501-31502	CD	denotes	9
T6438	31502-31503	-RRB-	denotes	]
T6439	31503-31504	.	denotes	.
T6440	31504-31629	sentence	denotes	When this domain was overexpressed in serum-starved keratinocytes, a comparable elevation in pMAPK was observed (Figure 4F).
T6441	31505-31509	WRB	denotes	When
T6443	31510-31514	DT	denotes	this
T6444	31515-31521	NN	denotes	domain
T6445	31522-31525	VBD	denotes	was
T6442	31526-31539	VBN	denotes	overexpressed
T6447	31540-31542	IN	denotes	in
T6448	31543-31548	NN	denotes	serum
T6450	31548-31549	HYPH	denotes	-
T6449	31549-31556	VBN	denotes	starved
T6451	31557-31570	NNS	denotes	keratinocytes
T6452	31570-31572	,	denotes	,
T6453	31572-31573	DT	denotes	a
T6455	31574-31584	JJ	denotes	comparable
T6454	31585-31594	NN	denotes	elevation
T6456	31595-31597	IN	denotes	in
T6457	31598-31603	NN	denotes	pMAPK
T6458	31604-31607	VBD	denotes	was
T6446	31608-31616	VBN	denotes	observed
T6459	31617-31618	-LRB-	denotes	(
T6461	31618-31624	NN	denotes	Figure
T6460	31625-31627	NN	denotes	4F
T6462	31627-31628	-RRB-	denotes	)
T6463	31628-31629	.	denotes	.
T6464	31629-31733	sentence	denotes	As expected, the small peptide inhibitor that interrupts the Grb-2/Sos association blocked the effects.
T6465	31630-31632	IN	denotes	As
T6466	31633-31641	VBN	denotes	expected
T6468	31641-31643	,	denotes	,
T6469	31643-31646	DT	denotes	the
T6471	31647-31652	JJ	denotes	small
T6472	31653-31660	NN	denotes	peptide
T6470	31661-31670	NN	denotes	inhibitor
T6473	31671-31675	WDT	denotes	that
T6474	31676-31686	VBZ	denotes	interrupts
T6475	31687-31690	DT	denotes	the
T6477	31691-31694	NN	denotes	Grb
T6478	31694-31695	HYPH	denotes	-
T6479	31695-31696	CD	denotes	2
T6480	31696-31697	HYPH	denotes	/
T6481	31697-31700	NN	denotes	Sos
T6476	31701-31712	NN	denotes	association
T6467	31713-31720	VBD	denotes	blocked
T6482	31721-31724	DT	denotes	the
T6483	31725-31732	NNS	denotes	effects
T6484	31732-31733	.	denotes	.
T6485	31733-31958	sentence	denotes	These data suggested that by elevating cytosolic Ajuba levels, Ajuba's pre-LIM domain may associate with Grb-2/Sos in a manner that stimulates its nucleotide exchange activity and leads to activation of the Ras-MAPK pathway.
T6486	31734-31739	DT	denotes	These
T6487	31740-31744	NNS	denotes	data
T6488	31745-31754	VBD	denotes	suggested
T6489	31755-31759	IN	denotes	that
T6491	31760-31762	IN	denotes	by
T6492	31763-31772	VBG	denotes	elevating
T6493	31773-31782	JJ	denotes	cytosolic
T6495	31783-31788	NN	denotes	Ajuba
T6494	31789-31795	NNS	denotes	levels
T6496	31795-31797	,	denotes	,
T6497	31797-31802	NN	denotes	Ajuba
T6499	31802-31804	POS	denotes	's
T6500	31805-31812	JJ	denotes	pre-LIM
T6498	31813-31819	NN	denotes	domain
T6501	31820-31823	MD	denotes	may
T6490	31824-31833	VB	denotes	associate
T6502	31834-31838	IN	denotes	with
T6503	31839-31842	NN	denotes	Grb
T6504	31842-31843	HYPH	denotes	-
T6505	31843-31844	CD	denotes	2
T6506	31844-31845	HYPH	denotes	/
T6507	31845-31848	NN	denotes	Sos
T6508	31849-31851	IN	denotes	in
T6509	31852-31853	DT	denotes	a
T6510	31854-31860	NN	denotes	manner
T6511	31861-31865	WDT	denotes	that
T6512	31866-31876	VBZ	denotes	stimulates
T6513	31877-31880	PRP$	denotes	its
T6515	31881-31891	NN	denotes	nucleotide
T6516	31892-31900	NN	denotes	exchange
T6514	31901-31909	NN	denotes	activity
T6517	31910-31913	CC	denotes	and
T6518	31914-31919	VBZ	denotes	leads
T6519	31920-31922	IN	denotes	to
T6520	31923-31933	NN	denotes	activation
T6521	31934-31936	IN	denotes	of
T6522	31937-31940	DT	denotes	the
T6524	31941-31944	NN	denotes	Ras
T6526	31944-31945	HYPH	denotes	-
T6525	31945-31949	NN	denotes	MAPK
T6523	31950-31957	NN	denotes	pathway
T6527	31957-31958	.	denotes	.
T6528	31958-32168	sentence	denotes	Although this pathway provides one mechanism by which Snail expression and proliferation may be coupled in skin epithelium, proliferative circuitries involving AJs are known to be complex and often interwoven.
T6529	31959-31967	IN	denotes	Although
T6531	31968-31972	DT	denotes	this
T6532	31973-31980	NN	denotes	pathway
T6530	31981-31989	VBZ	denotes	provides
T6534	31990-31993	CD	denotes	one
T6535	31994-32003	NN	denotes	mechanism
T6536	32004-32006	IN	denotes	by
T6538	32007-32012	WDT	denotes	which
T6539	32013-32018	NN	denotes	Snail
T6540	32019-32029	NN	denotes	expression
T6541	32030-32033	CC	denotes	and
T6542	32034-32047	NN	denotes	proliferation
T6543	32048-32051	MD	denotes	may
T6544	32052-32054	VB	denotes	be
T6537	32055-32062	VBN	denotes	coupled
T6545	32063-32065	IN	denotes	in
T6546	32066-32070	NN	denotes	skin
T6547	32071-32081	NN	denotes	epithelium
T6548	32081-32083	,	denotes	,
T6549	32083-32096	JJ	denotes	proliferative
T6550	32097-32108	NNS	denotes	circuitries
T6551	32109-32118	VBG	denotes	involving
T6552	32119-32122	NNS	denotes	AJs
T6553	32123-32126	VBP	denotes	are
T6533	32127-32132	VBN	denotes	known
T6554	32133-32135	TO	denotes	to
T6555	32136-32138	VB	denotes	be
T6556	32139-32146	JJ	denotes	complex
T6557	32147-32150	CC	denotes	and
T6558	32151-32156	RB	denotes	often
T6559	32157-32167	VBN	denotes	interwoven
T6560	32167-32168	.	denotes	.
T6561	32168-32273	sentence	denotes	Future studies will be needed to systematically dissect these putative intricacies at a molecular level.
T6562	32169-32175	JJ	denotes	Future
T6563	32176-32183	NNS	denotes	studies
T6565	32184-32188	MD	denotes	will
T6566	32189-32191	VB	denotes	be
T6564	32192-32198	VBN	denotes	needed
T6567	32199-32201	TO	denotes	to
T6569	32202-32216	RB	denotes	systematically
T6568	32217-32224	VB	denotes	dissect
T6570	32225-32230	DT	denotes	these
T6572	32231-32239	JJ	denotes	putative
T6571	32240-32251	NNS	denotes	intricacies
T6573	32252-32254	IN	denotes	at
T6574	32255-32256	DT	denotes	a
T6576	32257-32266	JJ	denotes	molecular
T6575	32267-32272	NN	denotes	level
T6577	32272-32273	.	denotes	.
T7354	32275-32282	VBG	denotes	Probing
T7356	32283-32286	DT	denotes	the
T7357	32287-32297	NN	denotes	Regulation
T7358	32298-32300	IN	denotes	of
T7359	32301-32306	NN	denotes	Snail
T7360	32307-32311	NN	denotes	Gene
T7361	32312-32322	NN	denotes	Expression
T7355	32323-32330	VBZ	denotes	Reveals
T7362	32331-32333	DT	denotes	an
T7364	32334-32343	JJ	denotes	Essential
T7363	32344-32348	NN	denotes	Link
T7365	32349-32351	IN	denotes	to
T7366	32352-32355	NN	denotes	TGF
T7368	32355-32356	HYPH	denotes	-
T7367	32356-32358	NN	denotes	β2
T7369	32359-32368	NN	denotes	Signaling
T7370	32369-32371	IN	denotes	in
T7371	32372-32375	DT	denotes	the
T7373	32376-32386	VBG	denotes	Developing
T7374	32387-32391	NN	denotes	Hair
T7372	32392-32395	NN	denotes	Bud
T7375	32395-32528	sentence	denotes	The temporal spike of Snail mRNA expression in the hair bud prompted us to consider what factor(s) may be regulating the Snail gene.
T7376	32396-32399	DT	denotes	The
T7378	32400-32408	JJ	denotes	temporal
T7377	32409-32414	NN	denotes	spike
T7380	32415-32417	IN	denotes	of
T7381	32418-32423	NN	denotes	Snail
T7383	32424-32428	NN	denotes	mRNA
T7382	32429-32439	NN	denotes	expression
T7384	32440-32442	IN	denotes	in
T7385	32443-32446	DT	denotes	the
T7387	32447-32451	NN	denotes	hair
T7386	32452-32455	NN	denotes	bud
T7379	32456-32464	VBD	denotes	prompted
T7388	32465-32467	PRP	denotes	us
T7389	32468-32470	TO	denotes	to
T7390	32471-32479	VB	denotes	consider
T7391	32480-32484	WDT	denotes	what
T7392	32485-32491	NN	denotes	factor
T7394	32491-32492	-LRB-	denotes	(
T7395	32492-32493	AFX	denotes	s
T7396	32493-32494	-RRB-	denotes	)
T7397	32495-32498	MD	denotes	may
T7398	32499-32501	VB	denotes	be
T7393	32502-32512	VBG	denotes	regulating
T7399	32513-32516	DT	denotes	the
T7401	32517-32522	NN	denotes	Snail
T7400	32523-32527	NN	denotes	gene
T7402	32527-32528	.	denotes	.
T7403	32528-32752	sentence	denotes	A variety of extracellular signals have an impact on the cell type-specific expression of different Snail family members, and many of them, including Wnts, BMPs, FGFs, and TGF-βs, also affect hair bud development [2,16,28].
T7404	32529-32530	DT	denotes	A
T7405	32531-32538	NN	denotes	variety
T7407	32539-32541	IN	denotes	of
T7408	32542-32555	JJ	denotes	extracellular
T7409	32556-32563	NNS	denotes	signals
T7406	32564-32568	VBP	denotes	have
T7410	32569-32571	DT	denotes	an
T7411	32572-32578	NN	denotes	impact
T7412	32579-32581	IN	denotes	on
T7413	32582-32585	DT	denotes	the
T7415	32586-32590	NN	denotes	cell
T7416	32591-32595	NN	denotes	type
T7418	32595-32596	HYPH	denotes	-
T7417	32596-32604	JJ	denotes	specific
T7414	32605-32615	NN	denotes	expression
T7419	32616-32618	IN	denotes	of
T7420	32619-32628	JJ	denotes	different
T7422	32629-32634	NN	denotes	Snail
T7423	32635-32641	NN	denotes	family
T7421	32642-32649	NNS	denotes	members
T7424	32649-32651	,	denotes	,
T7425	32651-32654	CC	denotes	and
T7426	32655-32659	JJ	denotes	many
T7428	32660-32662	IN	denotes	of
T7429	32663-32667	PRP	denotes	them
T7430	32667-32669	,	denotes	,
T7431	32669-32678	VBG	denotes	including
T7432	32679-32683	NNS	denotes	Wnts
T7433	32683-32685	,	denotes	,
T7434	32685-32689	NNS	denotes	BMPs
T7435	32689-32691	,	denotes	,
T7436	32691-32695	NNS	denotes	FGFs
T7437	32695-32697	,	denotes	,
T7438	32697-32700	CC	denotes	and
T7439	32701-32704	NN	denotes	TGF
T7441	32704-32705	HYPH	denotes	-
T7440	32705-32707	NNS	denotes	βs
T7442	32707-32709	,	denotes	,
T7443	32709-32713	RB	denotes	also
T7427	32714-32720	VBP	denotes	affect
T7444	32721-32725	NN	denotes	hair
T7445	32726-32729	NN	denotes	bud
T7446	32730-32741	NN	denotes	development
T7447	32742-32743	-LRB-	denotes	[
T7449	32743-32744	CD	denotes	2
T7450	32744-32745	,	denotes	,
T7451	32745-32747	CD	denotes	16
T7452	32747-32748	,	denotes	,
T7448	32748-32750	CD	denotes	28
T7453	32750-32751	-RRB-	denotes	]
T7454	32751-32752	.	denotes	.
T7455	32752-32980	sentence	denotes	Since Snail is not expressed in cultured skin keratinocytes that secrete active BMPs and FGFs (see Figure 1B), we focused our attention on Wnt and TGF-β signaling as more likely candidates for Snail induction in this cell type.
T7456	32753-32758	IN	denotes	Since
T7458	32759-32764	NN	denotes	Snail
T7459	32765-32767	VBZ	denotes	is
T7460	32768-32771	RB	denotes	not
T7457	32772-32781	VBN	denotes	expressed
T7462	32782-32784	IN	denotes	in
T7463	32785-32793	VBN	denotes	cultured
T7465	32794-32798	NN	denotes	skin
T7464	32799-32812	NNS	denotes	keratinocytes
T7466	32813-32817	WDT	denotes	that
T7467	32818-32825	VBP	denotes	secrete
T7468	32826-32832	JJ	denotes	active
T7469	32833-32837	NNS	denotes	BMPs
T7470	32838-32841	CC	denotes	and
T7471	32842-32846	NNS	denotes	FGFs
T7472	32847-32848	-LRB-	denotes	(
T7473	32848-32851	VB	denotes	see
T7474	32852-32858	NN	denotes	Figure
T7475	32859-32861	NN	denotes	1B
T7476	32861-32862	-RRB-	denotes	)
T7477	32862-32864	,	denotes	,
T7478	32864-32866	PRP	denotes	we
T7461	32867-32874	VBD	denotes	focused
T7479	32875-32878	PRP$	denotes	our
T7480	32879-32888	NN	denotes	attention
T7481	32889-32891	IN	denotes	on
T7482	32892-32895	NN	denotes	Wnt
T7484	32896-32899	CC	denotes	and
T7485	32900-32903	NN	denotes	TGF
T7487	32903-32904	HYPH	denotes	-
T7486	32904-32905	NN	denotes	β
T7483	32906-32915	NN	denotes	signaling
T7488	32916-32918	IN	denotes	as
T7489	32919-32923	RBR	denotes	more
T7490	32924-32930	JJ	denotes	likely
T7491	32931-32941	NNS	denotes	candidates
T7492	32942-32945	IN	denotes	for
T7493	32946-32951	NN	denotes	Snail
T7494	32952-32961	NN	denotes	induction
T7495	32962-32964	IN	denotes	in
T7496	32965-32969	DT	denotes	this
T7498	32970-32974	NN	denotes	cell
T7497	32975-32979	NN	denotes	type
T7499	32979-32980	.	denotes	.
T7500	32980-33179	sentence	denotes	Previously, we showed that effective transmission of a Wnt-3a signal in cultured keratinocytes can be achieved through their exposure to the BMP inhibitor noggin, which induces LEF-1 expression [4].
T7501	32981-32991	RB	denotes	Previously
T7503	32991-32993	,	denotes	,
T7504	32993-32995	PRP	denotes	we
T7502	32996-33002	VBD	denotes	showed
T7505	33003-33007	IN	denotes	that
T7507	33008-33017	JJ	denotes	effective
T7508	33018-33030	NN	denotes	transmission
T7509	33031-33033	IN	denotes	of
T7510	33034-33035	DT	denotes	a
T7512	33036-33039	NN	denotes	Wnt
T7513	33039-33040	HYPH	denotes	-
T7514	33040-33042	CD	denotes	3a
T7511	33043-33049	NN	denotes	signal
T7515	33050-33052	IN	denotes	in
T7516	33053-33061	VBN	denotes	cultured
T7517	33062-33075	NNS	denotes	keratinocytes
T7518	33076-33079	MD	denotes	can
T7519	33080-33082	VB	denotes	be
T7506	33083-33091	VBN	denotes	achieved
T7520	33092-33099	IN	denotes	through
T7521	33100-33105	PRP$	denotes	their
T7522	33106-33114	NN	denotes	exposure
T7523	33115-33117	IN	denotes	to
T7524	33118-33121	DT	denotes	the
T7526	33122-33125	NN	denotes	BMP
T7525	33126-33135	NN	denotes	inhibitor
T7527	33136-33142	NN	denotes	noggin
T7528	33142-33144	,	denotes	,
T7529	33144-33149	WDT	denotes	which
T7530	33150-33157	VBZ	denotes	induces
T7531	33158-33161	NN	denotes	LEF
T7533	33161-33162	HYPH	denotes	-
T7534	33162-33163	CD	denotes	1
T7532	33164-33174	NN	denotes	expression
T7535	33175-33176	-LRB-	denotes	[
T7536	33176-33177	CD	denotes	4
T7537	33177-33178	-RRB-	denotes	]
T7538	33178-33179	.	denotes	.
T7539	33179-33314	sentence	denotes	In vitro, these conditions down-regulated the E-cadherin promoter and induced a LEF-1/β-catenin-sensitive reporter gene, TOPFLASH [4].
T7540	33180-33182	FW	denotes	In
T7541	33183-33188	FW	denotes	vitro
T7543	33188-33190	,	denotes	,
T7544	33190-33195	DT	denotes	these
T7545	33196-33206	NNS	denotes	conditions
T7546	33207-33211	RB	denotes	down
T7547	33211-33212	HYPH	denotes	-
T7542	33212-33221	VBD	denotes	regulated
T7548	33222-33225	DT	denotes	the
T7550	33226-33227	NN	denotes	E
T7552	33227-33228	HYPH	denotes	-
T7551	33228-33236	NN	denotes	cadherin
T7549	33237-33245	NN	denotes	promoter
T7553	33246-33249	CC	denotes	and
T7554	33250-33257	VBD	denotes	induced
T7555	33258-33259	DT	denotes	a
T7557	33260-33263	NN	denotes	LEF
T7559	33263-33264	HYPH	denotes	-
T7560	33264-33265	CD	denotes	1
T7561	33265-33266	HYPH	denotes	/
T7562	33266-33267	NN	denotes	β
T7564	33267-33268	HYPH	denotes	-
T7563	33268-33275	NN	denotes	catenin
T7565	33275-33276	HYPH	denotes	-
T7558	33276-33285	JJ	denotes	sensitive
T7566	33286-33294	NN	denotes	reporter
T7556	33295-33299	NN	denotes	gene
T7567	33299-33301	,	denotes	,
T7568	33301-33309	NN	denotes	TOPFLASH
T7569	33310-33311	-LRB-	denotes	[
T7570	33311-33312	CD	denotes	4
T7571	33312-33313	-RRB-	denotes	]
T7572	33313-33314	.	denotes	.
T7573	33314-33393	sentence	denotes	In contrast, Snail expression was not induced by these conditions (Figure 5A).
T7574	33315-33317	IN	denotes	In
T7576	33318-33326	NN	denotes	contrast
T7577	33326-33328	,	denotes	,
T7578	33328-33333	NN	denotes	Snail
T7579	33334-33344	NN	denotes	expression
T7580	33345-33348	VBD	denotes	was
T7581	33349-33352	RB	denotes	not
T7575	33353-33360	VBN	denotes	induced
T7582	33361-33363	IN	denotes	by
T7583	33364-33369	DT	denotes	these
T7584	33370-33380	NNS	denotes	conditions
T7585	33381-33382	-LRB-	denotes	(
T7587	33382-33388	NN	denotes	Figure
T7586	33389-33391	NN	denotes	5A
T7588	33391-33392	-RRB-	denotes	)
T7589	33392-33393	.	denotes	.
T7590	33393-33597	sentence	denotes	Thus, despite essential roles for Wnts and noggin in hair follicle specification [4,29,30], our studies did not support an essential role for these signals in governing Snail expression in keratinocytes.
T7591	33394-33398	RB	denotes	Thus
T7593	33398-33400	,	denotes	,
T7594	33400-33407	IN	denotes	despite
T7595	33408-33417	JJ	denotes	essential
T7596	33418-33423	NNS	denotes	roles
T7597	33424-33427	IN	denotes	for
T7598	33428-33432	NNS	denotes	Wnts
T7599	33433-33436	CC	denotes	and
T7600	33437-33443	NN	denotes	noggin
T7601	33444-33446	IN	denotes	in
T7602	33447-33451	NN	denotes	hair
T7603	33452-33460	NN	denotes	follicle
T7604	33461-33474	NN	denotes	specification
T7605	33475-33476	-LRB-	denotes	[
T7607	33476-33477	CD	denotes	4
T7608	33477-33478	,	denotes	,
T7609	33478-33480	CD	denotes	29
T7610	33480-33481	,	denotes	,
T7606	33481-33483	CD	denotes	30
T7611	33483-33484	-RRB-	denotes	]
T7612	33484-33486	,	denotes	,
T7613	33486-33489	PRP$	denotes	our
T7614	33490-33497	NNS	denotes	studies
T7615	33498-33501	VBD	denotes	did
T7616	33502-33505	RB	denotes	not
T7592	33506-33513	VB	denotes	support
T7617	33514-33516	DT	denotes	an
T7619	33517-33526	JJ	denotes	essential
T7618	33527-33531	NN	denotes	role
T7620	33532-33535	IN	denotes	for
T7621	33536-33541	DT	denotes	these
T7622	33542-33549	NNS	denotes	signals
T7623	33550-33552	IN	denotes	in
T7624	33553-33562	VBG	denotes	governing
T7625	33563-33568	NN	denotes	Snail
T7626	33569-33579	NN	denotes	expression
T7627	33580-33582	IN	denotes	in
T7628	33583-33596	NNS	denotes	keratinocytes
T7629	33596-33597	.	denotes	.
T7630	33597-35796	sentence	denotes	Figure 5 TGF-β2, but Not Wnt/noggin, Transiently Induces Snail Expression In Vitro (A) Failure of Wnt and noggin signaling to induce Snail in cultured keratinocytes. Primary mouse keratinocytes were treated with Wnt- and/or noggin-conditioned medium (+) or the corresponding control medium (–). These conditions are known to activate the LEF-1/β-catenin reporter TOPGal and down-regulate the E-cadherin promoter (see [4] for details). Using Western blot analyses, cellular proteins were then analyzed for Snail, LEF-1, β-catenin, and tubulin. Proteins from keratinocytes transfected with K14-Snail were used as a positive control for Snail expression. (B) TGF-β2 can induce Snail protein. Primary keratinocytes were treated for the indicated times with recombinant TGF-β2 (+) or heat inactivated TGF-β2 (–).Total cellular proteins were then isolated and analyzed by Western blot for Snail, pSMAD2 (reflective of activated TGF- signaling), and tubulin. Note the activation of Snail expression, peaking at 2 h post-TGF-β2 treatment and then disappearing thereafter. (C) Snail mRNA expression is transiently induced by TGF-β2. The experiment in (B) was repeated, and this time, total RNAs were isolated from keratinocytes treated with TGF-β2 for the indicated times. RT-PCR was then used with (+) or without (–) reverse transcriptase (RT) and with primer sets specific for Snail and GAPDH mRNAs. Note that Snail mRNA expression also peaked at 2 h, paralleling Snail protein. (D) TGF-β2 treatment results in enhanced activity of a Snail promoter-β-galactosidase reporter. Keratinocytes were transfected with a β-galactosidase reporter driven by a Snail promoter that is either WT (wt prom) or harbors a mutation in a putative pSMAD2/pSMAD4 binding site (mt prom). At 2 d posttransfection, cells were treated with either TGF-β or heat-inactivated TGF-β2 (inact) for the times indicated. β-galactosidase assays were then conducted, and results are reported as fold increase over a basal level of activity of 1. The experiment was repeated three times in triplicate, and error bars reflect variations in the results. TGF-β1 has been shown to induce Snail family members in hepatocytes and heart [15, 31].
T7631	35709-35712	NN	denotes	TGF
T7633	35712-35713	HYPH	denotes	-
T7632	35713-35715	NN	denotes	β1
T7635	35716-35719	VBZ	denotes	has
T7636	35720-35724	VBN	denotes	been
T7634	35725-35730	VBN	denotes	shown
T7637	35731-35733	TO	denotes	to
T7638	35734-35740	VB	denotes	induce
T7639	35741-35746	NN	denotes	Snail
T7641	35747-35753	NN	denotes	family
T7640	35754-35761	NNS	denotes	members
T7642	35762-35764	IN	denotes	in
T7643	35765-35776	NNS	denotes	hepatocytes
T7644	35777-35780	CC	denotes	and
T7645	35781-35786	NN	denotes	heart
T7646	35787-35788	-LRB-	denotes	[
T7648	35788-35790	CD	denotes	15
T7649	35790-35792	,	denotes	,
T7647	35792-35794	CD	denotes	31
T7650	35794-35795	-RRB-	denotes	]
T7651	35795-35796	.	denotes	.
T7652	35796-35955	sentence	denotes	In keratinocytes, however, TGF-β1 inhibits keratinocyte growth and seems to be involved in triggering the destructive phase of the cycling hair follicle [32].
T7653	35797-35799	IN	denotes	In
T7655	35800-35813	NNS	denotes	keratinocytes
T7656	35813-35815	,	denotes	,
T7657	35815-35822	RB	denotes	however
T7658	35822-35824	,	denotes	,
T7659	35824-35827	NN	denotes	TGF
T7661	35827-35828	HYPH	denotes	-
T7660	35828-35830	NN	denotes	β1
T7654	35831-35839	VBZ	denotes	inhibits
T7662	35840-35852	NN	denotes	keratinocyte
T7663	35853-35859	NN	denotes	growth
T7664	35860-35863	CC	denotes	and
T7665	35864-35869	VBZ	denotes	seems
T7666	35870-35872	TO	denotes	to
T7668	35873-35875	VB	denotes	be
T7667	35876-35884	VBN	denotes	involved
T7669	35885-35887	IN	denotes	in
T7670	35888-35898	VBG	denotes	triggering
T7671	35899-35902	DT	denotes	the
T7673	35903-35914	JJ	denotes	destructive
T7672	35915-35920	NN	denotes	phase
T7674	35921-35923	IN	denotes	of
T7675	35924-35927	DT	denotes	the
T7677	35928-35935	VBG	denotes	cycling
T7678	35936-35940	NN	denotes	hair
T7676	35941-35949	NN	denotes	follicle
T7679	35950-35951	-LRB-	denotes	[
T7680	35951-35953	CD	denotes	32
T7681	35953-35954	-RRB-	denotes	]
T7682	35954-35955	.	denotes	.
T7683	35955-36111	sentence	denotes	Of the loss-of-function mutations generated in each of the TGF-β genes, only the TGF-β2 null state blocked follicle development at the hair bud stage [32].
T7684	35956-35958	IN	denotes	Of
T7686	35959-35962	DT	denotes	the
T7688	35963-35967	NN	denotes	loss
T7689	35967-35968	HYPH	denotes	-
T7690	35968-35970	IN	denotes	of
T7691	35970-35971	HYPH	denotes	-
T7692	35971-35979	NN	denotes	function
T7687	35980-35989	NNS	denotes	mutations
T7693	35990-35999	VBN	denotes	generated
T7694	36000-36002	IN	denotes	in
T7695	36003-36007	DT	denotes	each
T7696	36008-36010	IN	denotes	of
T7697	36011-36014	DT	denotes	the
T7699	36015-36018	NN	denotes	TGF
T7701	36018-36019	HYPH	denotes	-
T7700	36019-36020	NN	denotes	β
T7698	36021-36026	NNS	denotes	genes
T7702	36026-36028	,	denotes	,
T7703	36028-36032	RB	denotes	only
T7705	36033-36036	DT	denotes	the
T7706	36037-36040	NN	denotes	TGF
T7708	36040-36041	HYPH	denotes	-
T7707	36041-36043	NN	denotes	β2
T7709	36044-36048	JJ	denotes	null
T7704	36049-36054	NN	denotes	state
T7685	36055-36062	VBD	denotes	blocked
T7710	36063-36071	NN	denotes	follicle
T7711	36072-36083	NN	denotes	development
T7712	36084-36086	IN	denotes	at
T7713	36087-36090	DT	denotes	the
T7715	36091-36095	NN	denotes	hair
T7716	36096-36099	NN	denotes	bud
T7714	36100-36105	NN	denotes	stage
T7717	36106-36107	-LRB-	denotes	[
T7718	36107-36109	CD	denotes	32
T7719	36109-36110	-RRB-	denotes	]
T7720	36110-36111	.	denotes	.
T7721	36111-36275	sentence	denotes	Thus, we turned towards addressing whether TGF-β2 might be involved in regulating Snail expression in keratinocytes isolated from the basal layer of the epidermis.
T7722	36112-36116	RB	denotes	Thus
T7724	36116-36118	,	denotes	,
T7725	36118-36120	PRP	denotes	we
T7723	36121-36127	VBD	denotes	turned
T7726	36128-36135	IN	denotes	towards
T7727	36136-36146	VBG	denotes	addressing
T7728	36147-36154	IN	denotes	whether
T7730	36155-36158	NN	denotes	TGF
T7732	36158-36159	HYPH	denotes	-
T7731	36159-36161	NN	denotes	β2
T7733	36162-36167	MD	denotes	might
T7734	36168-36170	VB	denotes	be
T7729	36171-36179	VBN	denotes	involved
T7735	36180-36182	IN	denotes	in
T7736	36183-36193	VBG	denotes	regulating
T7737	36194-36199	NN	denotes	Snail
T7738	36200-36210	NN	denotes	expression
T7739	36211-36213	IN	denotes	in
T7740	36214-36227	NNS	denotes	keratinocytes
T7741	36228-36236	VBN	denotes	isolated
T7742	36237-36241	IN	denotes	from
T7743	36242-36245	DT	denotes	the
T7745	36246-36251	JJ	denotes	basal
T7744	36252-36257	NN	denotes	layer
T7746	36258-36260	IN	denotes	of
T7747	36261-36264	DT	denotes	the
T7748	36265-36274	NN	denotes	epidermis
T7749	36274-36275	.	denotes	.
T7750	36275-36506	sentence	denotes	Though there is no cell culture system available to specifically study placodal cells, these keratinocytes are their progenitors and are the closest approximation available to study the behavior of epithelial cells of the placode.
T7751	36276-36282	IN	denotes	Though
T7753	36283-36288	EX	denotes	there
T7752	36289-36291	VBZ	denotes	is
T7755	36292-36294	DT	denotes	no
T7757	36295-36299	NN	denotes	cell
T7758	36300-36307	NN	denotes	culture
T7756	36308-36314	NN	denotes	system
T7759	36315-36324	JJ	denotes	available
T7760	36325-36327	TO	denotes	to
T7762	36328-36340	RB	denotes	specifically
T7761	36341-36346	VB	denotes	study
T7763	36347-36355	JJ	denotes	placodal
T7764	36356-36361	NNS	denotes	cells
T7765	36361-36363	,	denotes	,
T7766	36363-36368	DT	denotes	these
T7767	36369-36382	NNS	denotes	keratinocytes
T7754	36383-36386	VBP	denotes	are
T7768	36387-36392	PRP$	denotes	their
T7769	36393-36404	NNS	denotes	progenitors
T7770	36405-36408	CC	denotes	and
T7771	36409-36412	VBP	denotes	are
T7772	36413-36416	DT	denotes	the
T7774	36417-36424	JJS	denotes	closest
T7773	36425-36438	NN	denotes	approximation
T7775	36439-36448	JJ	denotes	available
T7776	36449-36451	TO	denotes	to
T7777	36452-36457	VB	denotes	study
T7778	36458-36461	DT	denotes	the
T7779	36462-36470	NN	denotes	behavior
T7780	36471-36473	IN	denotes	of
T7781	36474-36484	JJ	denotes	epithelial
T7782	36485-36490	NNS	denotes	cells
T7783	36491-36493	IN	denotes	of
T7784	36494-36497	DT	denotes	the
T7785	36498-36505	NN	denotes	placode
T7786	36505-36506	.	denotes	.
T7787	36506-36654	sentence	denotes	Interestingly, treatment of cultured keratinocytes with as little as 5 ng/ml of TGF-β2 caused a rapid and transient induction of Snail (Figure 5B).
T7788	36507-36520	RB	denotes	Interestingly
T7790	36520-36522	,	denotes	,
T7791	36522-36531	NN	denotes	treatment
T7792	36532-36534	IN	denotes	of
T7793	36535-36543	VBN	denotes	cultured
T7794	36544-36557	NNS	denotes	keratinocytes
T7795	36558-36562	IN	denotes	with
T7796	36563-36565	RB	denotes	as
T7798	36566-36572	JJ	denotes	little
T7799	36573-36575	IN	denotes	as
T7797	36576-36577	CD	denotes	5
T7800	36578-36580	NN	denotes	ng
T7801	36580-36581	SYM	denotes	/
T7802	36581-36583	NN	denotes	ml
T7803	36584-36586	IN	denotes	of
T7804	36587-36590	NN	denotes	TGF
T7806	36590-36591	HYPH	denotes	-
T7805	36591-36593	NN	denotes	β2
T7789	36594-36600	VBD	denotes	caused
T7807	36601-36602	DT	denotes	a
T7809	36603-36608	JJ	denotes	rapid
T7810	36609-36612	CC	denotes	and
T7811	36613-36622	JJ	denotes	transient
T7808	36623-36632	NN	denotes	induction
T7812	36633-36635	IN	denotes	of
T7813	36636-36641	NN	denotes	Snail
T7814	36642-36643	-LRB-	denotes	(
T7816	36643-36649	NN	denotes	Figure
T7815	36650-36652	NN	denotes	5B
T7817	36652-36653	-RRB-	denotes	)
T7818	36653-36654	.	denotes	.
T7819	36654-36767	sentence	denotes	Following this treatment, Snail protein was detected within 30 min, peaked at 2 h, and then declined thereafter.
T7820	36655-36664	VBG	denotes	Following
T7822	36665-36669	DT	denotes	this
T7823	36670-36679	NN	denotes	treatment
T7824	36679-36681	,	denotes	,
T7825	36681-36686	NN	denotes	Snail
T7826	36687-36694	NN	denotes	protein
T7827	36695-36698	VBD	denotes	was
T7821	36699-36707	VBN	denotes	detected
T7828	36708-36714	IN	denotes	within
T7829	36715-36717	CD	denotes	30
T7830	36718-36721	NN	denotes	min
T7831	36721-36723	,	denotes	,
T7832	36723-36729	VBD	denotes	peaked
T7833	36730-36732	IN	denotes	at
T7834	36733-36734	CD	denotes	2
T7835	36735-36736	NN	denotes	h
T7836	36736-36738	,	denotes	,
T7837	36738-36741	CC	denotes	and
T7838	36742-36746	RB	denotes	then
T7839	36747-36755	VBD	denotes	declined
T7840	36756-36766	RB	denotes	thereafter
T7841	36766-36767	.	denotes	.
T7842	36767-36959	sentence	denotes	The induction of Snail appeared to be specific for the active form of the growth factor, as pretreatment of TGF-β2 for 10 min at 100 °C obliterated the response [Figure 5B, lanes marked (–)].
T7843	36768-36771	DT	denotes	The
T7844	36772-36781	NN	denotes	induction
T7846	36782-36784	IN	denotes	of
T7847	36785-36790	NN	denotes	Snail
T7845	36791-36799	VBD	denotes	appeared
T7848	36800-36802	TO	denotes	to
T7849	36803-36805	VB	denotes	be
T7850	36806-36814	JJ	denotes	specific
T7851	36815-36818	IN	denotes	for
T7852	36819-36822	DT	denotes	the
T7854	36823-36829	JJ	denotes	active
T7853	36830-36834	NN	denotes	form
T7855	36835-36837	IN	denotes	of
T7856	36838-36841	DT	denotes	the
T7858	36842-36848	NN	denotes	growth
T7857	36849-36855	NN	denotes	factor
T7859	36855-36857	,	denotes	,
T7860	36857-36859	IN	denotes	as
T7862	36860-36872	NN	denotes	pretreatment
T7863	36873-36875	IN	denotes	of
T7864	36876-36879	NN	denotes	TGF
T7866	36879-36880	HYPH	denotes	-
T7865	36880-36882	NN	denotes	β2
T7867	36883-36886	IN	denotes	for
T7868	36887-36889	CD	denotes	10
T7869	36890-36893	NN	denotes	min
T7870	36894-36896	IN	denotes	at
T7871	36897-36900	CD	denotes	100
T7872	36901-36903	NN	denotes	°C
T7861	36904-36915	VBD	denotes	obliterated
T7873	36916-36919	DT	denotes	the
T7874	36920-36928	NN	denotes	response
T7875	36929-36930	-LRB-	denotes	[
T7877	36930-36936	NN	denotes	Figure
T7878	36937-36939	NN	denotes	5B
T7879	36939-36941	,	denotes	,
T7876	36941-36946	NNS	denotes	lanes
T7880	36947-36953	VBN	denotes	marked
T7881	36954-36955	-LRB-	denotes	(
T7882	36955-36956	SYM	denotes	–
T7883	36956-36957	-RRB-	denotes	)
T7884	36957-36958	-RRB-	denotes	]
T7885	36958-36959	.	denotes	.
T7886	36959-37197	sentence	denotes	By contrast, although TGF-β receptor activation remained elevated during the duration of the experiment (as measured by the sustained phosphorylation of the downstream effector SMAD2) Snail expression could not be maintained (Figure 5B).
T7887	36960-36962	IN	denotes	By
T7889	36963-36971	NN	denotes	contrast
T7890	36971-36973	,	denotes	,
T7891	36973-36981	IN	denotes	although
T7893	36982-36985	NN	denotes	TGF
T7895	36985-36986	HYPH	denotes	-
T7894	36986-36987	NN	denotes	β
T7896	36988-36996	NN	denotes	receptor
T7897	36997-37007	NN	denotes	activation
T7892	37008-37016	VBD	denotes	remained
T7898	37017-37025	JJ	denotes	elevated
T7899	37026-37032	IN	denotes	during
T7900	37033-37036	DT	denotes	the
T7901	37037-37045	NN	denotes	duration
T7902	37046-37048	IN	denotes	of
T7903	37049-37052	DT	denotes	the
T7904	37053-37063	NN	denotes	experiment
T7905	37064-37065	-LRB-	denotes	(
T7906	37065-37067	IN	denotes	as
T7907	37068-37076	VBN	denotes	measured
T7908	37077-37079	IN	denotes	by
T7909	37080-37083	DT	denotes	the
T7911	37084-37093	JJ	denotes	sustained
T7910	37094-37109	NN	denotes	phosphorylation
T7912	37110-37112	IN	denotes	of
T7913	37113-37116	DT	denotes	the
T7915	37117-37127	JJ	denotes	downstream
T7914	37128-37136	NN	denotes	effector
T7916	37137-37142	NN	denotes	SMAD2
T7917	37142-37143	-RRB-	denotes	)
T7918	37144-37149	NN	denotes	Snail
T7919	37150-37160	NN	denotes	expression
T7920	37161-37166	MD	denotes	could
T7921	37167-37170	RB	denotes	not
T7922	37171-37173	VB	denotes	be
T7888	37174-37184	VBN	denotes	maintained
T7923	37185-37186	-LRB-	denotes	(
T7925	37186-37192	NN	denotes	Figure
T7924	37193-37195	NN	denotes	5B
T7926	37195-37196	-RRB-	denotes	)
T7927	37196-37197	.	denotes	.
T7928	37197-37345	sentence	denotes	Thus, although Snail expression correlated with phosphorylated SMAD2 (pSMAD2) induction, its decline seemed to rely on secondary downstream events.
T7929	37198-37202	RB	denotes	Thus
T7931	37202-37204	,	denotes	,
T7932	37204-37212	IN	denotes	although
T7934	37213-37218	NN	denotes	Snail
T7935	37219-37229	NN	denotes	expression
T7933	37230-37240	VBD	denotes	correlated
T7936	37241-37245	IN	denotes	with
T7937	37246-37260	VBN	denotes	phosphorylated
T7938	37261-37266	NN	denotes	SMAD2
T7940	37267-37268	-LRB-	denotes	(
T7941	37268-37274	NN	denotes	pSMAD2
T7942	37274-37275	-RRB-	denotes	)
T7939	37276-37285	NN	denotes	induction
T7943	37285-37287	,	denotes	,
T7944	37287-37290	PRP$	denotes	its
T7945	37291-37298	NN	denotes	decline
T7930	37299-37305	VBD	denotes	seemed
T7946	37306-37308	TO	denotes	to
T7947	37309-37313	VB	denotes	rely
T7948	37314-37316	IN	denotes	on
T7949	37317-37326	JJ	denotes	secondary
T7951	37327-37337	JJ	denotes	downstream
T7950	37338-37344	NNS	denotes	events
T7952	37344-37345	.	denotes	.
T7953	37345-37527	sentence	denotes	The rapid kinetics of Snail expression were reflected at the mRNA level, suggesting that Snail promoter activity in keratinocytes might be sensitive to TGF-β2 signaling (Figure 5C).
T7954	37346-37349	DT	denotes	The
T7956	37350-37355	JJ	denotes	rapid
T7955	37356-37364	NNS	denotes	kinetics
T7958	37365-37367	IN	denotes	of
T7959	37368-37373	NN	denotes	Snail
T7960	37374-37384	NN	denotes	expression
T7961	37385-37389	VBD	denotes	were
T7957	37390-37399	VBN	denotes	reflected
T7962	37400-37402	IN	denotes	at
T7963	37403-37406	DT	denotes	the
T7965	37407-37411	NN	denotes	mRNA
T7964	37412-37417	NN	denotes	level
T7966	37417-37419	,	denotes	,
T7967	37419-37429	VBG	denotes	suggesting
T7968	37430-37434	IN	denotes	that
T7970	37435-37440	NN	denotes	Snail
T7971	37441-37449	NN	denotes	promoter
T7972	37450-37458	NN	denotes	activity
T7973	37459-37461	IN	denotes	in
T7974	37462-37475	NNS	denotes	keratinocytes
T7975	37476-37481	MD	denotes	might
T7969	37482-37484	VB	denotes	be
T7976	37485-37494	JJ	denotes	sensitive
T7977	37495-37497	IN	denotes	to
T7978	37498-37501	NN	denotes	TGF
T7980	37501-37502	HYPH	denotes	-
T7979	37502-37504	NN	denotes	β2
T7981	37505-37514	NN	denotes	signaling
T7982	37515-37516	-LRB-	denotes	(
T7984	37516-37522	NN	denotes	Figure
T7983	37523-37525	NN	denotes	5C
T7985	37525-37526	-RRB-	denotes	)
T7986	37526-37527	.	denotes	.
T7987	37527-37754	sentence	denotes	To test this possibility, we engineered a transgene driving the β-galactosidase reporter under the control of approximately 2.2 kb of promoter sequence located 5′ from the transcription initiation site of the mouse Snail gene.
T7988	37528-37530	TO	denotes	To
T7989	37531-37535	VB	denotes	test
T7991	37536-37540	DT	denotes	this
T7992	37541-37552	NN	denotes	possibility
T7993	37552-37554	,	denotes	,
T7994	37554-37556	PRP	denotes	we
T7990	37557-37567	VBD	denotes	engineered
T7995	37568-37569	DT	denotes	a
T7996	37570-37579	NN	denotes	transgene
T7997	37580-37587	VBG	denotes	driving
T7998	37588-37591	DT	denotes	the
T8000	37592-37593	NN	denotes	β
T8002	37593-37594	HYPH	denotes	-
T8001	37594-37607	NN	denotes	galactosidase
T7999	37608-37616	NN	denotes	reporter
T8003	37617-37622	IN	denotes	under
T8004	37623-37626	DT	denotes	the
T8005	37627-37634	NN	denotes	control
T8006	37635-37637	IN	denotes	of
T8007	37638-37651	RB	denotes	approximately
T8008	37652-37655	CD	denotes	2.2
T8009	37656-37658	NN	denotes	kb
T8010	37659-37661	IN	denotes	of
T8011	37662-37670	NN	denotes	promoter
T8012	37671-37679	NN	denotes	sequence
T8013	37680-37687	VBN	denotes	located
T8014	37688-37689	CD	denotes	5
T8015	37689-37690	SYM	denotes	′
T8016	37691-37695	IN	denotes	from
T8017	37696-37699	DT	denotes	the
T8019	37700-37713	NN	denotes	transcription
T8020	37714-37724	NN	denotes	initiation
T8018	37725-37729	NN	denotes	site
T8021	37730-37732	IN	denotes	of
T8022	37733-37736	DT	denotes	the
T8024	37737-37742	NN	denotes	mouse
T8025	37743-37748	NN	denotes	Snail
T8023	37749-37753	NN	denotes	gene
T8026	37753-37754	.	denotes	.
T8027	37754-37954	sentence	denotes	At 2 d after transient transfection, keratinocytes were treated with TGF-β2 (t = 0) and then assayed for transgene activity over the same time course in which we had observed Snail protein induction.
T8028	37755-37757	IN	denotes	At
T8030	37758-37759	CD	denotes	2
T8031	37760-37761	NN	denotes	d
T8032	37762-37767	IN	denotes	after
T8033	37768-37777	JJ	denotes	transient
T8034	37778-37790	NN	denotes	transfection
T8035	37790-37792	,	denotes	,
T8036	37792-37805	NNS	denotes	keratinocytes
T8037	37806-37810	VBD	denotes	were
T8029	37811-37818	VBN	denotes	treated
T8038	37819-37823	IN	denotes	with
T8039	37824-37827	NN	denotes	TGF
T8041	37827-37828	HYPH	denotes	-
T8040	37828-37830	NN	denotes	β2
T8042	37831-37832	-LRB-	denotes	(
T8044	37832-37833	NN	denotes	t
T8045	37834-37835	SYM	denotes	=
T8043	37836-37837	CD	denotes	0
T8046	37837-37838	-RRB-	denotes	)
T8047	37839-37842	CC	denotes	and
T8048	37843-37847	RB	denotes	then
T8049	37848-37855	VBN	denotes	assayed
T8050	37856-37859	IN	denotes	for
T8051	37860-37869	NN	denotes	transgene
T8052	37870-37878	NN	denotes	activity
T8053	37879-37883	IN	denotes	over
T8054	37884-37887	DT	denotes	the
T8056	37888-37892	JJ	denotes	same
T8057	37893-37897	NN	denotes	time
T8055	37898-37904	NN	denotes	course
T8058	37905-37907	IN	denotes	in
T8060	37908-37913	WDT	denotes	which
T8061	37914-37916	PRP	denotes	we
T8062	37917-37920	VBD	denotes	had
T8059	37921-37929	VBN	denotes	observed
T8063	37930-37935	NN	denotes	Snail
T8064	37936-37943	NN	denotes	protein
T8065	37944-37953	NN	denotes	induction
T8066	37953-37954	.	denotes	.
T8067	37954-38013	sentence	denotes	The results of this experiment are presented in Figure 5D.
T8068	37955-37958	DT	denotes	The
T8069	37959-37966	NNS	denotes	results
T8071	37967-37969	IN	denotes	of
T8072	37970-37974	DT	denotes	this
T8073	37975-37985	NN	denotes	experiment
T8074	37986-37989	VBP	denotes	are
T8070	37990-37999	VBN	denotes	presented
T8075	38000-38002	IN	denotes	in
T8076	38003-38009	NN	denotes	Figure
T8077	38010-38012	NN	denotes	5D
T8078	38012-38013	.	denotes	.
T8079	38013-38173	sentence	denotes	Within 0.5 h of TGF-β2 treatment, Snail promoter activity had increased 3-fold, and by 2 h, it peaked to approximately 10-fold over control levels (Figure 5D).
T8080	38014-38020	IN	denotes	Within
T8082	38021-38024	CD	denotes	0.5
T8083	38025-38026	NN	denotes	h
T8084	38027-38029	IN	denotes	of
T8085	38030-38033	NN	denotes	TGF
T8087	38033-38034	HYPH	denotes	-
T8086	38034-38036	NN	denotes	β2
T8088	38037-38046	NN	denotes	treatment
T8089	38046-38048	,	denotes	,
T8090	38048-38053	NN	denotes	Snail
T8091	38054-38062	NN	denotes	promoter
T8092	38063-38071	NN	denotes	activity
T8093	38072-38075	VBD	denotes	had
T8081	38076-38085	VBN	denotes	increased
T8094	38086-38092	RB	denotes	3-fold
T8095	38092-38094	,	denotes	,
T8096	38094-38097	CC	denotes	and
T8097	38098-38100	IN	denotes	by
T8099	38101-38102	CD	denotes	2
T8100	38103-38104	NN	denotes	h
T8101	38104-38106	,	denotes	,
T8102	38106-38108	PRP	denotes	it
T8098	38109-38115	VBD	denotes	peaked
T8103	38116-38118	IN	denotes	to
T8104	38119-38132	RB	denotes	approximately
T8105	38133-38140	RB	denotes	10-fold
T8106	38141-38145	IN	denotes	over
T8107	38146-38153	NN	denotes	control
T8108	38154-38160	NNS	denotes	levels
T8109	38161-38162	-LRB-	denotes	(
T8111	38162-38168	NN	denotes	Figure
T8110	38169-38171	NN	denotes	5D
T8112	38171-38172	-RRB-	denotes	)
T8113	38172-38173	.	denotes	.
T8114	38173-38282	sentence	denotes	Thereafter, Snail promoter activity rapidly returned to the basal levels seen in unstimulated keratinocytes.
T8115	38174-38184	RB	denotes	Thereafter
T8117	38184-38186	,	denotes	,
T8118	38186-38191	NN	denotes	Snail
T8119	38192-38200	NN	denotes	promoter
T8120	38201-38209	NN	denotes	activity
T8121	38210-38217	RB	denotes	rapidly
T8116	38218-38226	VBD	denotes	returned
T8122	38227-38229	IN	denotes	to
T8123	38230-38233	DT	denotes	the
T8125	38234-38239	JJ	denotes	basal
T8124	38240-38246	NNS	denotes	levels
T8126	38247-38251	VBN	denotes	seen
T8127	38252-38254	IN	denotes	in
T8128	38255-38267	JJ	denotes	unstimulated
T8129	38268-38281	NNS	denotes	keratinocytes
T8130	38281-38282	.	denotes	.
T8131	38282-38385	sentence	denotes	The kinetics of Snail promoter activity closely paralleled those observed for Snail protein induction.
T8132	38283-38286	DT	denotes	The
T8133	38287-38295	NNS	denotes	kinetics
T8135	38296-38298	IN	denotes	of
T8136	38299-38304	NN	denotes	Snail
T8137	38305-38313	NN	denotes	promoter
T8138	38314-38322	NN	denotes	activity
T8139	38323-38330	RB	denotes	closely
T8134	38331-38341	VBD	denotes	paralleled
T8140	38342-38347	DT	denotes	those
T8141	38348-38356	VBN	denotes	observed
T8142	38357-38360	IN	denotes	for
T8143	38361-38366	NN	denotes	Snail
T8144	38367-38374	NN	denotes	protein
T8145	38375-38384	NN	denotes	induction
T8146	38384-38385	.	denotes	.
T8147	38385-38657	sentence	denotes	Moreover, the stimulatory effects appeared to be specific to TGF-β2, since they were abrogated either by heat inactivation of the TGF-β2 protein or by mutation of a putative SMAD binding element located about 1.8 kb 5′ from the Snail transcription start site (Figure 5D).
T8148	38386-38394	RB	denotes	Moreover
T8150	38394-38396	,	denotes	,
T8151	38396-38399	DT	denotes	the
T8153	38400-38411	JJ	denotes	stimulatory
T8152	38412-38419	NNS	denotes	effects
T8149	38420-38428	VBD	denotes	appeared
T8154	38429-38431	TO	denotes	to
T8155	38432-38434	VB	denotes	be
T8156	38435-38443	JJ	denotes	specific
T8157	38444-38446	IN	denotes	to
T8158	38447-38450	NN	denotes	TGF
T8160	38450-38451	HYPH	denotes	-
T8159	38451-38453	NN	denotes	β2
T8161	38453-38455	,	denotes	,
T8162	38455-38460	IN	denotes	since
T8164	38461-38465	PRP	denotes	they
T8165	38466-38470	VBD	denotes	were
T8163	38471-38480	VBN	denotes	abrogated
T8166	38481-38487	CC	denotes	either
T8167	38488-38490	IN	denotes	by
T8168	38491-38495	NN	denotes	heat
T8169	38496-38508	NN	denotes	inactivation
T8170	38509-38511	IN	denotes	of
T8171	38512-38515	DT	denotes	the
T8173	38516-38519	NN	denotes	TGF
T8175	38519-38520	HYPH	denotes	-
T8174	38520-38522	NN	denotes	β2
T8172	38523-38530	NN	denotes	protein
T8176	38531-38533	CC	denotes	or
T8177	38534-38536	IN	denotes	by
T8178	38537-38545	NN	denotes	mutation
T8179	38546-38548	IN	denotes	of
T8180	38549-38550	DT	denotes	a
T8182	38551-38559	JJ	denotes	putative
T8183	38560-38564	NN	denotes	SMAD
T8184	38565-38572	NN	denotes	binding
T8181	38573-38580	NN	denotes	element
T8185	38581-38588	VBN	denotes	located
T8186	38589-38594	IN	denotes	about
T8187	38595-38598	CD	denotes	1.8
T8188	38599-38601	NN	denotes	kb
T8189	38602-38603	CD	denotes	5
T8190	38603-38604	SYM	denotes	′
T8191	38605-38609	IN	denotes	from
T8192	38610-38613	DT	denotes	the
T8194	38614-38619	NN	denotes	Snail
T8195	38620-38633	NN	denotes	transcription
T8196	38634-38639	NN	denotes	start
T8193	38640-38644	NN	denotes	site
T8197	38645-38646	-LRB-	denotes	(
T8199	38646-38652	NN	denotes	Figure
T8198	38653-38655	NN	denotes	5D
T8200	38655-38656	-RRB-	denotes	)
T8201	38656-38657	.	denotes	.
T8202	38657-38896	sentence	denotes	Taken together, these results suggested that in keratinocytes, TGF-β2 signaling results in a pSMAD2-dependent transient activation of the Snail gene, and that maintenance of Snail protein relies, in part, upon sustained promoter activity.
T8203	38658-38663	VBN	denotes	Taken
T8205	38664-38672	RB	denotes	together
T8206	38672-38674	,	denotes	,
T8207	38674-38679	DT	denotes	these
T8208	38680-38687	NNS	denotes	results
T8204	38688-38697	VBD	denotes	suggested
T8209	38698-38702	IN	denotes	that
T8211	38703-38705	IN	denotes	in
T8212	38706-38719	NNS	denotes	keratinocytes
T8213	38719-38721	,	denotes	,
T8214	38721-38724	NN	denotes	TGF
T8216	38724-38725	HYPH	denotes	-
T8215	38725-38727	NN	denotes	β2
T8217	38728-38737	NN	denotes	signaling
T8210	38738-38745	VBZ	denotes	results
T8218	38746-38748	IN	denotes	in
T8219	38749-38750	DT	denotes	a
T8221	38751-38757	NN	denotes	pSMAD2
T8223	38757-38758	HYPH	denotes	-
T8222	38758-38767	JJ	denotes	dependent
T8224	38768-38777	JJ	denotes	transient
T8220	38778-38788	NN	denotes	activation
T8225	38789-38791	IN	denotes	of
T8226	38792-38795	DT	denotes	the
T8228	38796-38801	NN	denotes	Snail
T8227	38802-38806	NN	denotes	gene
T8229	38806-38808	,	denotes	,
T8230	38808-38811	CC	denotes	and
T8231	38812-38816	IN	denotes	that
T8233	38817-38828	NN	denotes	maintenance
T8234	38829-38831	IN	denotes	of
T8235	38832-38837	NN	denotes	Snail
T8236	38838-38845	NN	denotes	protein
T8232	38846-38852	VBZ	denotes	relies
T8237	38852-38854	,	denotes	,
T8238	38854-38856	IN	denotes	in
T8239	38857-38861	NN	denotes	part
T8240	38861-38863	,	denotes	,
T8241	38863-38867	IN	denotes	upon
T8242	38868-38877	JJ	denotes	sustained
T8244	38878-38886	NN	denotes	promoter
T8243	38887-38895	NN	denotes	activity
T8245	38895-38896	.	denotes	.
T8246	38896-39119	sentence	denotes	The brevity of Snail gene and protein induction in TGF-β2 treated cultured keratinocytes resembled the temporal appearance of Snail mRNA and protein at the initiation of hair follicle morphogenesis in embryonic mouse skin.
T8247	38897-38900	DT	denotes	The
T8248	38901-38908	NN	denotes	brevity
T8250	38909-38911	IN	denotes	of
T8251	38912-38917	NN	denotes	Snail
T8252	38918-38922	NN	denotes	gene
T8254	38923-38926	CC	denotes	and
T8255	38927-38934	NN	denotes	protein
T8253	38935-38944	NN	denotes	induction
T8256	38945-38947	IN	denotes	in
T8257	38948-38951	NN	denotes	TGF
T8259	38951-38952	HYPH	denotes	-
T8258	38952-38954	NN	denotes	β2
T8260	38955-38962	VBN	denotes	treated
T8262	38963-38971	VBN	denotes	cultured
T8261	38972-38985	NNS	denotes	keratinocytes
T8249	38986-38995	VBD	denotes	resembled
T8263	38996-38999	DT	denotes	the
T8265	39000-39008	JJ	denotes	temporal
T8264	39009-39019	NN	denotes	appearance
T8266	39020-39022	IN	denotes	of
T8267	39023-39028	NN	denotes	Snail
T8268	39029-39033	NN	denotes	mRNA
T8269	39034-39037	CC	denotes	and
T8270	39038-39045	NN	denotes	protein
T8271	39046-39048	IN	denotes	at
T8272	39049-39052	DT	denotes	the
T8273	39053-39063	NN	denotes	initiation
T8274	39064-39066	IN	denotes	of
T8275	39067-39071	NN	denotes	hair
T8276	39072-39080	NN	denotes	follicle
T8277	39081-39094	NN	denotes	morphogenesis
T8278	39095-39097	IN	denotes	in
T8279	39098-39107	JJ	denotes	embryonic
T8281	39108-39113	NN	denotes	mouse
T8280	39114-39118	NN	denotes	skin
T8282	39118-39119	.	denotes	.
T8283	39119-39285	sentence	denotes	To test whether TGF-β2 might be required for Snail induction in hair bud formation in vivo, we first analyzed whether TGF-β2 was expressed in or around the hair bud.
T8284	39120-39122	TO	denotes	To
T8285	39123-39127	VB	denotes	test
T8287	39128-39135	IN	denotes	whether
T8289	39136-39139	NN	denotes	TGF
T8291	39139-39140	HYPH	denotes	-
T8290	39140-39142	NN	denotes	β2
T8292	39143-39148	MD	denotes	might
T8293	39149-39151	VB	denotes	be
T8288	39152-39160	VBN	denotes	required
T8294	39161-39164	IN	denotes	for
T8295	39165-39170	NN	denotes	Snail
T8296	39171-39180	NN	denotes	induction
T8297	39181-39183	IN	denotes	in
T8298	39184-39188	NN	denotes	hair
T8299	39189-39192	NN	denotes	bud
T8300	39193-39202	NN	denotes	formation
T8301	39203-39205	FW	denotes	in
T8302	39206-39210	FW	denotes	vivo
T8303	39210-39212	,	denotes	,
T8304	39212-39214	PRP	denotes	we
T8305	39215-39220	RB	denotes	first
T8286	39221-39229	VBD	denotes	analyzed
T8306	39230-39237	IN	denotes	whether
T8308	39238-39241	NN	denotes	TGF
T8310	39241-39242	HYPH	denotes	-
T8309	39242-39244	NN	denotes	β2
T8311	39245-39248	VBD	denotes	was
T8307	39249-39258	VBN	denotes	expressed
T8312	39259-39261	IN	denotes	in
T8313	39262-39264	CC	denotes	or
T8314	39265-39271	IN	denotes	around
T8315	39272-39275	DT	denotes	the
T8317	39276-39280	NN	denotes	hair
T8316	39281-39284	NN	denotes	bud
T8318	39284-39285	.	denotes	.
T8319	39285-39395	sentence	denotes	Consistent with previous observations [33], an anti-TGF-β2 antibody labeled developing hair buds (Figure 6A).
T8320	39286-39296	JJ	denotes	Consistent
T8322	39297-39301	IN	denotes	with
T8323	39302-39310	JJ	denotes	previous
T8324	39311-39323	NNS	denotes	observations
T8325	39324-39325	-LRB-	denotes	[
T8326	39325-39327	CD	denotes	33
T8327	39327-39328	-RRB-	denotes	]
T8328	39328-39330	,	denotes	,
T8329	39330-39332	DT	denotes	an
T8331	39333-39341	JJ	denotes	anti-TGF
T8333	39341-39342	HYPH	denotes	-
T8332	39342-39344	NN	denotes	β2
T8330	39345-39353	NN	denotes	antibody
T8321	39354-39361	VBD	denotes	labeled
T8334	39362-39372	VBG	denotes	developing
T8336	39373-39377	NN	denotes	hair
T8335	39378-39382	NNS	denotes	buds
T8337	39383-39384	-LRB-	denotes	(
T8339	39384-39390	NN	denotes	Figure
T8338	39391-39393	NN	denotes	6A
T8340	39393-39394	-RRB-	denotes	)
T8341	39394-39395	.	denotes	.
T8342	39395-39551	sentence	denotes	This labeling appeared to be specific as judged by the lack of staining in follicle buds from mice homozygous for a TGF-β2 null mutation (Figure 6A; [34]).
T8343	39396-39400	DT	denotes	This
T8344	39401-39409	NN	denotes	labeling
T8345	39410-39418	VBD	denotes	appeared
T8346	39419-39421	TO	denotes	to
T8347	39422-39424	VB	denotes	be
T8348	39425-39433	JJ	denotes	specific
T8349	39434-39436	IN	denotes	as
T8350	39437-39443	VBN	denotes	judged
T8351	39444-39446	IN	denotes	by
T8352	39447-39450	DT	denotes	the
T8353	39451-39455	NN	denotes	lack
T8354	39456-39458	IN	denotes	of
T8355	39459-39467	NN	denotes	staining
T8356	39468-39470	IN	denotes	in
T8357	39471-39479	NN	denotes	follicle
T8358	39480-39484	NNS	denotes	buds
T8359	39485-39489	IN	denotes	from
T8360	39490-39494	NNS	denotes	mice
T8361	39495-39505	JJ	denotes	homozygous
T8362	39506-39509	IN	denotes	for
T8363	39510-39511	DT	denotes	a
T8365	39512-39515	NN	denotes	TGF
T8367	39515-39516	HYPH	denotes	-
T8366	39516-39518	NN	denotes	β2
T8368	39519-39523	JJ	denotes	null
T8364	39524-39532	NN	denotes	mutation
T8369	39533-39534	-LRB-	denotes	(
T8371	39534-39540	NN	denotes	Figure
T8372	39541-39543	NN	denotes	6A
T8373	39543-39544	:	denotes	;
T8374	39545-39546	-LRB-	denotes	[
T8370	39546-39548	CD	denotes	34
T8375	39548-39549	-RRB-	denotes	]
T8376	39549-39550	-RRB-	denotes	)
T8377	39550-39551	.	denotes	.
T8378	39551-39684	sentence	denotes	Moreover, the downstream effector of TGF-β2 signaling, pSMAD2, was also expressed in WT, but not TGF-β2-null, hair buds (Figure 6B).
T8379	39552-39560	RB	denotes	Moreover
T8381	39560-39562	,	denotes	,
T8382	39562-39565	DT	denotes	the
T8384	39566-39576	JJ	denotes	downstream
T8383	39577-39585	NN	denotes	effector
T8385	39586-39588	IN	denotes	of
T8386	39589-39592	NN	denotes	TGF
T8388	39592-39593	HYPH	denotes	-
T8387	39593-39595	NN	denotes	β2
T8389	39596-39605	NN	denotes	signaling
T8390	39605-39607	,	denotes	,
T8391	39607-39613	NN	denotes	pSMAD2
T8392	39613-39615	,	denotes	,
T8393	39615-39618	VBD	denotes	was
T8394	39619-39623	RB	denotes	also
T8380	39624-39633	VBN	denotes	expressed
T8395	39634-39636	IN	denotes	in
T8396	39637-39639	NN	denotes	WT
T8398	39639-39641	,	denotes	,
T8399	39641-39644	CC	denotes	but
T8400	39645-39648	RB	denotes	not
T8401	39649-39652	NN	denotes	TGF
T8403	39652-39653	HYPH	denotes	-
T8402	39653-39655	NN	denotes	β2
T8405	39655-39656	HYPH	denotes	-
T8404	39656-39660	JJ	denotes	null
T8406	39660-39662	,	denotes	,
T8407	39662-39666	NN	denotes	hair
T8397	39667-39671	NNS	denotes	buds
T8408	39672-39673	-LRB-	denotes	(
T8410	39673-39679	NN	denotes	Figure
T8409	39680-39682	NN	denotes	6B
T8411	39682-39683	-RRB-	denotes	)
T8412	39683-39684	.	denotes	.
T8413	39684-39837	sentence	denotes	Together, these data underscore the importance of the TGF-β2 isoform despite expression of both TGF-β1 and TGF-β2 in developing hair buds at this stage.
T8414	39685-39693	RB	denotes	Together
T8416	39693-39695	,	denotes	,
T8417	39695-39700	DT	denotes	these
T8418	39701-39705	NNS	denotes	data
T8415	39706-39716	VBP	denotes	underscore
T8419	39717-39720	DT	denotes	the
T8420	39721-39731	NN	denotes	importance
T8421	39732-39734	IN	denotes	of
T8422	39735-39738	DT	denotes	the
T8424	39739-39742	NN	denotes	TGF
T8426	39742-39743	HYPH	denotes	-
T8425	39743-39745	NN	denotes	β2
T8423	39746-39753	NN	denotes	isoform
T8427	39754-39761	IN	denotes	despite
T8428	39762-39772	NN	denotes	expression
T8429	39773-39775	IN	denotes	of
T8430	39776-39780	CC	denotes	both
T8432	39781-39784	NN	denotes	TGF
T8433	39784-39785	HYPH	denotes	-
T8431	39785-39787	NN	denotes	β1
T8434	39788-39791	CC	denotes	and
T8435	39792-39795	NN	denotes	TGF
T8437	39795-39796	HYPH	denotes	-
T8436	39796-39798	NN	denotes	β2
T8438	39799-39801	IN	denotes	in
T8439	39802-39812	VBG	denotes	developing
T8441	39813-39817	NN	denotes	hair
T8440	39818-39822	NNS	denotes	buds
T8442	39823-39825	IN	denotes	at
T8443	39826-39830	DT	denotes	this
T8444	39831-39836	NN	denotes	stage
T8445	39836-39837	.	denotes	.
T8446	39837-40968	sentence	denotes	Figure 6 TGF-β2 Is Necessary to Induce Snail Expression and Regulate Proliferation and E-Cadherin in the Hair Bud (A–D) Skins from TGF-β2 WT or KO E17.5 embryos were analyzed for expression of TGF-β2 protein (A), which is present in the epidermis and dermis as previously described [33] and in the hair bud, pSMAD2 (B), Snail (C), and Snail mRNA (D). Arrows point to the hair buds. (E) Western blot analyses of Snail expression in the skins of 2-wk-old K14-Smad2 transgenic (SMAD2 TG) and WT littermate (WT) mice. Antibody to tubulin was used as a control for equal protein loadings. The K14-Smad2 Tg mouse was previously shown to possess activated TGF-β signaling [35]. (F–G) Proliferation markers Ki67 (F) and pMAPK (G) are diminished in TGF-β2-null hair relative to its WT counterpart. (H–J) TGF-β2-null hair fails to down-regulate E-cadherin (H). Wnt and noggin signaling pathways are still intact in the TGF-β2 null hair as nuclear LEF-1 (I) and nuclear β-catenin (J) are still expressed. To further explore the possible relation between Snail and TGF-β2, we examined the status of Snail expression in TGF-β2-null hair buds.
T8447	40833-40835	TO	denotes	To
T8449	40836-40843	RB	denotes	further
T8448	40844-40851	VB	denotes	explore
T8451	40852-40855	DT	denotes	the
T8453	40856-40864	JJ	denotes	possible
T8452	40865-40873	NN	denotes	relation
T8454	40874-40881	IN	denotes	between
T8455	40882-40887	NN	denotes	Snail
T8456	40888-40891	CC	denotes	and
T8457	40892-40895	NN	denotes	TGF
T8459	40895-40896	HYPH	denotes	-
T8458	40896-40898	NN	denotes	β2
T8460	40898-40900	,	denotes	,
T8461	40900-40902	PRP	denotes	we
T8450	40903-40911	VBD	denotes	examined
T8462	40912-40915	DT	denotes	the
T8463	40916-40922	NN	denotes	status
T8464	40923-40925	IN	denotes	of
T8465	40926-40931	NN	denotes	Snail
T8466	40932-40942	NN	denotes	expression
T8467	40943-40945	IN	denotes	in
T8468	40946-40949	NN	denotes	TGF
T8470	40949-40950	HYPH	denotes	-
T8469	40950-40952	NN	denotes	β2
T8472	40952-40953	HYPH	denotes	-
T8471	40953-40957	JJ	denotes	null
T8474	40958-40962	NN	denotes	hair
T8473	40963-40967	NNS	denotes	buds
T8475	40967-40968	.	denotes	.
T8476	40968-41121	sentence	denotes	As judged by immunohistochemistry, Snail protein was absent from E17.5 skin of TGF-β2-null embryos but not from that of control littermates (Figure 6C).
T8477	40969-40971	IN	denotes	As
T8478	40972-40978	VBN	denotes	judged
T8480	40979-40981	IN	denotes	by
T8481	40982-41002	NN	denotes	immunohistochemistry
T8482	41002-41004	,	denotes	,
T8483	41004-41009	NN	denotes	Snail
T8484	41010-41017	NN	denotes	protein
T8479	41018-41021	VBD	denotes	was
T8485	41022-41028	JJ	denotes	absent
T8486	41029-41033	IN	denotes	from
T8487	41034-41039	NN	denotes	E17.5
T8488	41040-41044	NN	denotes	skin
T8489	41045-41047	IN	denotes	of
T8490	41048-41051	NN	denotes	TGF
T8492	41051-41052	HYPH	denotes	-
T8491	41052-41054	NN	denotes	β2
T8494	41054-41055	HYPH	denotes	-
T8493	41055-41059	JJ	denotes	null
T8495	41060-41067	NNS	denotes	embryos
T8496	41068-41071	CC	denotes	but
T8497	41072-41075	RB	denotes	not
T8498	41076-41080	IN	denotes	from
T8499	41081-41085	DT	denotes	that
T8500	41086-41088	IN	denotes	of
T8501	41089-41096	NN	denotes	control
T8502	41097-41108	NNS	denotes	littermates
T8503	41109-41110	-LRB-	denotes	(
T8505	41110-41116	NN	denotes	Figure
T8504	41117-41119	NN	denotes	6C
T8506	41119-41120	-RRB-	denotes	)
T8507	41120-41121	.	denotes	.
T8508	41121-41356	sentence	denotes	This effect appeared to be exerted at the transcriptional level, since Snail mRNAs were also not found in TGF-β2 null hair buds under conditions in which the signal was readily detected in the hair buds of littermate skin (Figure 6D).
T8509	41122-41126	DT	denotes	This
T8510	41127-41133	NN	denotes	effect
T8511	41134-41142	VBD	denotes	appeared
T8512	41143-41145	TO	denotes	to
T8514	41146-41148	VB	denotes	be
T8513	41149-41156	VBN	denotes	exerted
T8515	41157-41159	IN	denotes	at
T8516	41160-41163	DT	denotes	the
T8518	41164-41179	JJ	denotes	transcriptional
T8517	41180-41185	NN	denotes	level
T8519	41185-41187	,	denotes	,
T8520	41187-41192	IN	denotes	since
T8522	41193-41198	NN	denotes	Snail
T8523	41199-41204	NNS	denotes	mRNAs
T8524	41205-41209	VBD	denotes	were
T8525	41210-41214	RB	denotes	also
T8526	41215-41218	RB	denotes	not
T8521	41219-41224	VBN	denotes	found
T8527	41225-41227	IN	denotes	in
T8528	41228-41231	NN	denotes	TGF
T8530	41231-41232	HYPH	denotes	-
T8529	41232-41234	NN	denotes	β2
T8531	41235-41239	JJ	denotes	null
T8533	41240-41244	NN	denotes	hair
T8532	41245-41249	NNS	denotes	buds
T8534	41250-41255	IN	denotes	under
T8535	41256-41266	NNS	denotes	conditions
T8536	41267-41269	IN	denotes	in
T8538	41270-41275	WDT	denotes	which
T8539	41276-41279	DT	denotes	the
T8540	41280-41286	NN	denotes	signal
T8541	41287-41290	VBD	denotes	was
T8542	41291-41298	RB	denotes	readily
T8537	41299-41307	VBN	denotes	detected
T8543	41308-41310	IN	denotes	in
T8544	41311-41314	DT	denotes	the
T8546	41315-41319	NN	denotes	hair
T8545	41320-41324	NNS	denotes	buds
T8547	41325-41327	IN	denotes	of
T8548	41328-41338	NN	denotes	littermate
T8549	41339-41343	NN	denotes	skin
T8550	41344-41345	-LRB-	denotes	(
T8552	41345-41351	NN	denotes	Figure
T8551	41352-41354	NN	denotes	6D
T8553	41354-41355	-RRB-	denotes	)
T8554	41355-41356	.	denotes	.
T8555	41356-41567	sentence	denotes	Conversely, in 2-wk-old K14-Smad2 Tg mice, which display elevated TGF-β signaling in skin [35], Snail protein was readily detected by Western blot analyses, where it was not found in postnatal skin (Figure 6E).
T8556	41357-41367	RB	denotes	Conversely
T8558	41367-41369	,	denotes	,
T8559	41369-41371	IN	denotes	in
T8560	41372-41373	CD	denotes	2
T8562	41373-41374	HYPH	denotes	-
T8561	41374-41376	NN	denotes	wk
T8564	41376-41377	HYPH	denotes	-
T8563	41377-41380	JJ	denotes	old
T8566	41381-41384	NN	denotes	K14
T8568	41384-41385	HYPH	denotes	-
T8567	41385-41390	NN	denotes	Smad2
T8569	41391-41393	NN	denotes	Tg
T8565	41394-41398	NNS	denotes	mice
T8570	41398-41400	,	denotes	,
T8571	41400-41405	WDT	denotes	which
T8572	41406-41413	VBP	denotes	display
T8573	41414-41422	VBN	denotes	elevated
T8575	41423-41426	NN	denotes	TGF
T8577	41426-41427	HYPH	denotes	-
T8576	41427-41428	NN	denotes	β
T8574	41429-41438	NN	denotes	signaling
T8578	41439-41441	IN	denotes	in
T8579	41442-41446	NN	denotes	skin
T8580	41447-41448	-LRB-	denotes	[
T8581	41448-41450	CD	denotes	35
T8582	41450-41451	-RRB-	denotes	]
T8583	41451-41453	,	denotes	,
T8584	41453-41458	NN	denotes	Snail
T8585	41459-41466	NN	denotes	protein
T8586	41467-41470	VBD	denotes	was
T8587	41471-41478	RB	denotes	readily
T8557	41479-41487	VBN	denotes	detected
T8588	41488-41490	IN	denotes	by
T8589	41491-41498	NNP	denotes	Western
T8590	41499-41503	NN	denotes	blot
T8591	41504-41512	NNS	denotes	analyses
T8592	41512-41514	,	denotes	,
T8593	41514-41519	WRB	denotes	where
T8595	41520-41522	PRP	denotes	it
T8596	41523-41526	VBD	denotes	was
T8597	41527-41530	RB	denotes	not
T8594	41531-41536	VBN	denotes	found
T8598	41537-41539	IN	denotes	in
T8599	41540-41549	JJ	denotes	postnatal
T8600	41550-41554	NN	denotes	skin
T8601	41555-41556	-LRB-	denotes	(
T8603	41556-41562	NN	denotes	Figure
T8602	41563-41565	NN	denotes	6E
T8604	41565-41566	-RRB-	denotes	)
T8605	41566-41567	.	denotes	.
T8606	41567-41751	sentence	denotes	Taken together, these results provide compelling evidence that TGF-β2 is functionally important for inducing Snail gene expression in a pSMAD-dependent manner in developing hair buds.
T8607	41568-41573	VBN	denotes	Taken
T8609	41574-41582	RB	denotes	together
T8610	41582-41584	,	denotes	,
T8611	41584-41589	DT	denotes	these
T8612	41590-41597	NNS	denotes	results
T8608	41598-41605	VBP	denotes	provide
T8613	41606-41616	JJ	denotes	compelling
T8614	41617-41625	NN	denotes	evidence
T8615	41626-41630	IN	denotes	that
T8617	41631-41634	NN	denotes	TGF
T8619	41634-41635	HYPH	denotes	-
T8618	41635-41637	NN	denotes	β2
T8616	41638-41640	VBZ	denotes	is
T8620	41641-41653	RB	denotes	functionally
T8621	41654-41663	JJ	denotes	important
T8622	41664-41667	IN	denotes	for
T8623	41668-41676	VBG	denotes	inducing
T8624	41677-41682	NN	denotes	Snail
T8626	41683-41687	NN	denotes	gene
T8625	41688-41698	NN	denotes	expression
T8627	41699-41701	IN	denotes	in
T8628	41702-41703	DT	denotes	a
T8630	41704-41709	NN	denotes	pSMAD
T8632	41709-41710	HYPH	denotes	-
T8631	41710-41719	JJ	denotes	dependent
T8629	41720-41726	NN	denotes	manner
T8633	41727-41729	IN	denotes	in
T8634	41730-41740	VBG	denotes	developing
T8636	41741-41745	NN	denotes	hair
T8635	41746-41750	NNS	denotes	buds
T8637	41750-41751	.	denotes	.
T8638	41751-41855	sentence	denotes	Whether pMARK activity also contributes to Snail induction was not addressed in the present study [15].
T8639	41752-41759	IN	denotes	Whether
T8641	41760-41765	NN	denotes	pMARK
T8642	41766-41774	NN	denotes	activity
T8643	41775-41779	RB	denotes	also
T8640	41780-41791	VBZ	denotes	contributes
T8645	41792-41794	IN	denotes	to
T8646	41795-41800	NN	denotes	Snail
T8647	41801-41810	NN	denotes	induction
T8648	41811-41814	VBD	denotes	was
T8649	41815-41818	RB	denotes	not
T8644	41819-41828	VBN	denotes	addressed
T8650	41829-41831	IN	denotes	in
T8651	41832-41835	DT	denotes	the
T8653	41836-41843	JJ	denotes	present
T8652	41844-41849	NN	denotes	study
T8654	41850-41851	-LRB-	denotes	[
T8655	41851-41853	CD	denotes	15
T8656	41853-41854	-RRB-	denotes	]
T8657	41854-41855	.	denotes	.
T8658	41855-41965	sentence	denotes	Although some hair buds still formed in TGF-β2 null skin, their number was reduced by approximately 50% [32].
T8659	41856-41864	IN	denotes	Although
T8661	41865-41869	DT	denotes	some
T8663	41870-41874	NN	denotes	hair
T8662	41875-41879	NNS	denotes	buds
T8664	41880-41885	RB	denotes	still
T8660	41886-41892	VBD	denotes	formed
T8666	41893-41895	IN	denotes	in
T8667	41896-41899	NN	denotes	TGF
T8669	41899-41900	HYPH	denotes	-
T8668	41900-41902	NN	denotes	β2
T8670	41903-41907	JJ	denotes	null
T8671	41908-41912	NN	denotes	skin
T8672	41912-41914	,	denotes	,
T8673	41914-41919	PRP$	denotes	their
T8674	41920-41926	NN	denotes	number
T8675	41927-41930	VBD	denotes	was
T8665	41931-41938	VBN	denotes	reduced
T8676	41939-41941	IN	denotes	by
T8677	41942-41955	RB	denotes	approximately
T8678	41956-41958	CD	denotes	50
T8679	41958-41959	NN	denotes	%
T8680	41960-41961	-LRB-	denotes	[
T8681	41961-41963	CD	denotes	32
T8682	41963-41964	-RRB-	denotes	]
T8683	41964-41965	.	denotes	.
T8684	41965-42133	sentence	denotes	Thus, although the pathway mediated by TGF-β2 signaling impacts the earliest step of epithelial invagination, it does not appear to be essential for bud morphogenesis.
T8685	41966-41970	RB	denotes	Thus
T8687	41970-41972	,	denotes	,
T8688	41972-41980	IN	denotes	although
T8690	41981-41984	DT	denotes	the
T8691	41985-41992	NN	denotes	pathway
T8692	41993-42001	VBN	denotes	mediated
T8693	42002-42004	IN	denotes	by
T8694	42005-42008	NN	denotes	TGF
T8696	42008-42009	HYPH	denotes	-
T8695	42009-42011	NN	denotes	β2
T8697	42012-42021	NN	denotes	signaling
T8689	42022-42029	VBZ	denotes	impacts
T8698	42030-42033	DT	denotes	the
T8700	42034-42042	JJS	denotes	earliest
T8699	42043-42047	NN	denotes	step
T8701	42048-42050	IN	denotes	of
T8702	42051-42061	JJ	denotes	epithelial
T8703	42062-42074	NN	denotes	invagination
T8704	42074-42076	,	denotes	,
T8705	42076-42078	PRP	denotes	it
T8706	42079-42083	VBZ	denotes	does
T8707	42084-42087	RB	denotes	not
T8686	42088-42094	VB	denotes	appear
T8708	42095-42097	TO	denotes	to
T8709	42098-42100	VB	denotes	be
T8710	42101-42110	JJ	denotes	essential
T8711	42111-42114	IN	denotes	for
T8712	42115-42118	NN	denotes	bud
T8713	42119-42132	NN	denotes	morphogenesis
T8714	42132-42133	.	denotes	.
T8715	42133-42407	sentence	denotes	Consistent with this notion, basement membrane remodeling still took place in the TGF-β2-null buds, as judged by immunofluorescence with antibodies against β4 integrin, an integral component of keratinocyte-mediated adhesion to its underlying basement membrane (Figure 6F).
T8716	42134-42144	JJ	denotes	Consistent
T8718	42145-42149	IN	denotes	with
T8719	42150-42154	DT	denotes	this
T8720	42155-42161	NN	denotes	notion
T8721	42161-42163	,	denotes	,
T8722	42163-42171	NN	denotes	basement
T8723	42172-42180	NN	denotes	membrane
T8724	42181-42191	NN	denotes	remodeling
T8725	42192-42197	RB	denotes	still
T8717	42198-42202	VBD	denotes	took
T8726	42203-42208	RP	denotes	place
T8727	42209-42211	IN	denotes	in
T8728	42212-42215	DT	denotes	the
T8730	42216-42219	NN	denotes	TGF
T8732	42219-42220	HYPH	denotes	-
T8731	42220-42222	NN	denotes	β2
T8734	42222-42223	HYPH	denotes	-
T8733	42223-42227	JJ	denotes	null
T8729	42228-42232	NNS	denotes	buds
T8735	42232-42234	,	denotes	,
T8736	42234-42236	IN	denotes	as
T8737	42237-42243	VBN	denotes	judged
T8738	42244-42246	IN	denotes	by
T8739	42247-42265	NN	denotes	immunofluorescence
T8740	42266-42270	IN	denotes	with
T8741	42271-42281	NNS	denotes	antibodies
T8742	42282-42289	IN	denotes	against
T8743	42290-42292	NN	denotes	β4
T8744	42293-42301	NN	denotes	integrin
T8745	42301-42303	,	denotes	,
T8746	42303-42305	DT	denotes	an
T8748	42306-42314	JJ	denotes	integral
T8747	42315-42324	NN	denotes	component
T8749	42325-42327	IN	denotes	of
T8750	42328-42340	NN	denotes	keratinocyte
T8752	42340-42341	HYPH	denotes	-
T8751	42341-42349	VBN	denotes	mediated
T8753	42350-42358	NN	denotes	adhesion
T8754	42359-42361	IN	denotes	to
T8755	42362-42365	PRP$	denotes	its
T8757	42366-42376	JJ	denotes	underlying
T8758	42377-42385	NN	denotes	basement
T8756	42386-42394	NN	denotes	membrane
T8759	42395-42396	-LRB-	denotes	(
T8761	42396-42402	NN	denotes	Figure
T8760	42403-42405	NN	denotes	6F
T8762	42405-42406	-RRB-	denotes	)
T8763	42406-42407	.	denotes	.
T8764	42407-42618	sentence	denotes	In contrast, TGF-β2 signaling appeared to be an important factor for the early proliferation that occurs in the developing hair buds, as judged by anti-Ki67 and anti-pMAPK immunofluorescence (Figure 6F and 6G).
T8765	42408-42410	IN	denotes	In
T8767	42411-42419	NN	denotes	contrast
T8768	42419-42421	,	denotes	,
T8769	42421-42424	NN	denotes	TGF
T8771	42424-42425	HYPH	denotes	-
T8770	42425-42427	NN	denotes	β2
T8772	42428-42437	NN	denotes	signaling
T8766	42438-42446	VBD	denotes	appeared
T8773	42447-42449	TO	denotes	to
T8774	42450-42452	VB	denotes	be
T8775	42453-42455	DT	denotes	an
T8777	42456-42465	JJ	denotes	important
T8776	42466-42472	NN	denotes	factor
T8778	42473-42476	IN	denotes	for
T8779	42477-42480	DT	denotes	the
T8781	42481-42486	JJ	denotes	early
T8780	42487-42500	NN	denotes	proliferation
T8782	42501-42505	WDT	denotes	that
T8783	42506-42512	VBZ	denotes	occurs
T8784	42513-42515	IN	denotes	in
T8785	42516-42519	DT	denotes	the
T8787	42520-42530	VBG	denotes	developing
T8788	42531-42535	NN	denotes	hair
T8786	42536-42540	NNS	denotes	buds
T8789	42540-42542	,	denotes	,
T8790	42542-42544	IN	denotes	as
T8791	42545-42551	VBN	denotes	judged
T8792	42552-42554	IN	denotes	by
T8793	42555-42564	JJ	denotes	anti-Ki67
T8795	42565-42568	CC	denotes	and
T8796	42569-42579	JJ	denotes	anti-pMAPK
T8794	42580-42598	NN	denotes	immunofluorescence
T8797	42599-42600	-LRB-	denotes	(
T8799	42600-42606	NN	denotes	Figure
T8798	42607-42609	NN	denotes	6F
T8800	42610-42613	CC	denotes	and
T8801	42614-42616	NN	denotes	6G
T8802	42616-42617	-RRB-	denotes	)
T8803	42617-42618	.	denotes	.
T8804	42618-42790	sentence	denotes	If TGF-β2 stimulates Snail expression in developing buds, loss of this morphogen would be expected to affect the expression of genes that are typically repressed by Snail.
T8805	42619-42621	IN	denotes	If
T8807	42622-42625	NN	denotes	TGF
T8809	42625-42626	HYPH	denotes	-
T8808	42626-42628	NN	denotes	β2
T8806	42629-42639	VBZ	denotes	stimulates
T8811	42640-42645	NN	denotes	Snail
T8812	42646-42656	NN	denotes	expression
T8813	42657-42659	IN	denotes	in
T8814	42660-42670	VBG	denotes	developing
T8815	42671-42675	NNS	denotes	buds
T8816	42675-42677	,	denotes	,
T8817	42677-42681	NN	denotes	loss
T8818	42682-42684	IN	denotes	of
T8819	42685-42689	DT	denotes	this
T8820	42690-42699	NN	denotes	morphogen
T8821	42700-42705	MD	denotes	would
T8822	42706-42708	VB	denotes	be
T8810	42709-42717	VBN	denotes	expected
T8823	42718-42720	TO	denotes	to
T8824	42721-42727	VB	denotes	affect
T8825	42728-42731	DT	denotes	the
T8826	42732-42742	NN	denotes	expression
T8827	42743-42745	IN	denotes	of
T8828	42746-42751	NNS	denotes	genes
T8829	42752-42756	WDT	denotes	that
T8831	42757-42760	VBP	denotes	are
T8832	42761-42770	RB	denotes	typically
T8830	42771-42780	VBN	denotes	repressed
T8833	42781-42783	IN	denotes	by
T8834	42784-42789	NN	denotes	Snail
T8835	42789-42790	.	denotes	.
T8836	42790-42935	sentence	denotes	Since a major target for Snail-mediated repression is the E-cadherin gene [12,13], we investigated the status of E-cadherin in TGF-β2-null buds.
T8837	42791-42796	IN	denotes	Since
T8839	42797-42798	DT	denotes	a
T8841	42799-42804	JJ	denotes	major
T8840	42805-42811	NN	denotes	target
T8842	42812-42815	IN	denotes	for
T8843	42816-42821	NN	denotes	Snail
T8845	42821-42822	HYPH	denotes	-
T8844	42822-42830	VBN	denotes	mediated
T8846	42831-42841	NN	denotes	repression
T8838	42842-42844	VBZ	denotes	is
T8848	42845-42848	DT	denotes	the
T8850	42849-42850	NN	denotes	E
T8852	42850-42851	HYPH	denotes	-
T8851	42851-42859	NN	denotes	cadherin
T8849	42860-42864	NN	denotes	gene
T8853	42865-42866	-LRB-	denotes	[
T8855	42866-42868	CD	denotes	12
T8856	42868-42869	,	denotes	,
T8854	42869-42871	CD	denotes	13
T8857	42871-42872	-RRB-	denotes	]
T8858	42872-42874	,	denotes	,
T8859	42874-42876	PRP	denotes	we
T8847	42877-42889	VBD	denotes	investigated
T8860	42890-42893	DT	denotes	the
T8861	42894-42900	NN	denotes	status
T8862	42901-42903	IN	denotes	of
T8863	42904-42905	NN	denotes	E
T8865	42905-42906	HYPH	denotes	-
T8864	42906-42914	NN	denotes	cadherin
T8866	42915-42917	IN	denotes	in
T8867	42918-42921	NN	denotes	TGF
T8869	42921-42922	HYPH	denotes	-
T8868	42922-42924	NN	denotes	β2
T8871	42924-42925	HYPH	denotes	-
T8870	42925-42929	JJ	denotes	null
T8872	42930-42934	NNS	denotes	buds
T8873	42934-42935	.	denotes	.
T8874	42935-43070	sentence	denotes	As shown in Figure 6H, hair buds in TGF-β2 null skin displayed elevated immunofluorescence staining relative to their WT counterparts.
T8875	42936-42938	IN	denotes	As
T8876	42939-42944	VBN	denotes	shown
T8878	42945-42947	IN	denotes	in
T8879	42948-42954	NN	denotes	Figure
T8880	42955-42957	NN	denotes	6H
T8881	42957-42959	,	denotes	,
T8882	42959-42963	NN	denotes	hair
T8883	42964-42968	NNS	denotes	buds
T8884	42969-42971	IN	denotes	in
T8885	42972-42975	NN	denotes	TGF
T8887	42975-42976	HYPH	denotes	-
T8886	42976-42978	NN	denotes	β2
T8888	42979-42983	JJ	denotes	null
T8889	42984-42988	NN	denotes	skin
T8877	42989-42998	VBD	denotes	displayed
T8890	42999-43007	JJ	denotes	elevated
T8892	43008-43026	NN	denotes	immunofluorescence
T8891	43027-43035	NN	denotes	staining
T8893	43036-43044	JJ	denotes	relative
T8894	43045-43047	IN	denotes	to
T8895	43048-43053	PRP$	denotes	their
T8897	43054-43056	NN	denotes	WT
T8896	43057-43069	NNS	denotes	counterparts
T8898	43069-43070	.	denotes	.
T8899	43070-43319	sentence	denotes	Previously we demonstrated that the concerted action of the extracellular signals Wnt and noggin are required for the generation of a LEF-1/β-catenin transcription complex to repress E-cadherin transcription at the onset of hair fate specification.
T8900	43071-43081	RB	denotes	Previously
T8902	43082-43084	PRP	denotes	we
T8901	43085-43097	VBD	denotes	demonstrated
T8903	43098-43102	IN	denotes	that
T8905	43103-43106	DT	denotes	the
T8907	43107-43116	JJ	denotes	concerted
T8906	43117-43123	NN	denotes	action
T8908	43124-43126	IN	denotes	of
T8909	43127-43130	DT	denotes	the
T8911	43131-43144	JJ	denotes	extracellular
T8910	43145-43152	NNS	denotes	signals
T8912	43153-43156	NN	denotes	Wnt
T8913	43157-43160	CC	denotes	and
T8914	43161-43167	NN	denotes	noggin
T8915	43168-43171	VBP	denotes	are
T8904	43172-43180	VBN	denotes	required
T8916	43181-43184	IN	denotes	for
T8918	43185-43188	DT	denotes	the
T8919	43189-43199	NN	denotes	generation
T8920	43200-43202	IN	denotes	of
T8921	43203-43204	DT	denotes	a
T8923	43205-43208	NN	denotes	LEF
T8924	43208-43209	HYPH	denotes	-
T8925	43209-43210	CD	denotes	1
T8926	43210-43211	HYPH	denotes	/
T8927	43211-43212	NN	denotes	β
T8929	43212-43213	HYPH	denotes	-
T8928	43213-43220	NN	denotes	catenin
T8930	43221-43234	NN	denotes	transcription
T8922	43235-43242	NN	denotes	complex
T8931	43243-43245	TO	denotes	to
T8917	43246-43253	VB	denotes	repress
T8932	43254-43255	NN	denotes	E
T8934	43255-43256	HYPH	denotes	-
T8933	43256-43264	NN	denotes	cadherin
T8935	43265-43278	NN	denotes	transcription
T8936	43279-43281	IN	denotes	at
T8937	43282-43285	DT	denotes	the
T8938	43286-43291	NN	denotes	onset
T8939	43292-43294	IN	denotes	of
T8940	43295-43299	NN	denotes	hair
T8941	43300-43304	NN	denotes	fate
T8942	43305-43318	NN	denotes	specification
T8943	43318-43319	.	denotes	.
T8944	43319-43486	sentence	denotes	As shown in Figure 6I and 6J, both WT and TGF-β2 null buds exhibited nuclear LEF-1 and β-catenin localization, signs that the Wnt-noggin signaling pathway was intact.
T8945	43320-43322	IN	denotes	As
T8946	43323-43328	VBN	denotes	shown
T8948	43329-43331	IN	denotes	in
T8949	43332-43338	NN	denotes	Figure
T8950	43339-43341	NN	denotes	6I
T8951	43342-43345	CC	denotes	and
T8952	43346-43348	NN	denotes	6J
T8953	43348-43350	,	denotes	,
T8954	43350-43354	CC	denotes	both
T8955	43355-43357	NN	denotes	WT
T8957	43358-43361	CC	denotes	and
T8958	43362-43365	NN	denotes	TGF
T8960	43365-43366	HYPH	denotes	-
T8959	43366-43368	NN	denotes	β2
T8956	43369-43373	JJ	denotes	null
T8961	43374-43378	NNS	denotes	buds
T8947	43379-43388	VBD	denotes	exhibited
T8962	43389-43396	JJ	denotes	nuclear
T8964	43397-43400	NN	denotes	LEF
T8965	43400-43401	HYPH	denotes	-
T8966	43401-43402	CD	denotes	1
T8967	43403-43406	CC	denotes	and
T8968	43407-43408	NN	denotes	β
T8970	43408-43409	HYPH	denotes	-
T8969	43409-43416	NN	denotes	catenin
T8963	43417-43429	NN	denotes	localization
T8971	43429-43431	,	denotes	,
T8972	43431-43436	NNS	denotes	signs
T8973	43437-43441	IN	denotes	that
T8975	43442-43445	DT	denotes	the
T8977	43446-43449	NN	denotes	Wnt
T8979	43449-43450	HYPH	denotes	-
T8978	43450-43456	NN	denotes	noggin
T8980	43457-43466	NN	denotes	signaling
T8976	43467-43474	NN	denotes	pathway
T8974	43475-43478	VBD	denotes	was
T8981	43479-43485	JJ	denotes	intact
T8982	43485-43486	.	denotes	.
T8983	43486-43627	sentence	denotes	These data suggest that during hair follicle morphogenesis, TGF-β2 functions subsequently to Wnt/noggin-mediated determination of hair fate.
T8984	43487-43492	DT	denotes	These
T8985	43493-43497	NNS	denotes	data
T8986	43498-43505	VBP	denotes	suggest
T8987	43506-43510	IN	denotes	that
T8989	43511-43517	IN	denotes	during
T8990	43518-43522	NN	denotes	hair
T8991	43523-43531	NN	denotes	follicle
T8992	43532-43545	NN	denotes	morphogenesis
T8993	43545-43547	,	denotes	,
T8994	43547-43550	NN	denotes	TGF
T8996	43550-43551	HYPH	denotes	-
T8995	43551-43553	NN	denotes	β2
T8988	43554-43563	VBZ	denotes	functions
T8997	43564-43576	RB	denotes	subsequently
T8998	43577-43579	IN	denotes	to
T8999	43580-43583	NN	denotes	Wnt
T9001	43583-43584	HYPH	denotes	/
T9000	43584-43590	NN	denotes	noggin
T9003	43590-43591	HYPH	denotes	-
T9002	43591-43599	VBN	denotes	mediated
T9004	43600-43613	NN	denotes	determination
T9005	43614-43616	IN	denotes	of
T9006	43617-43621	NN	denotes	hair
T9007	43622-43626	NN	denotes	fate
T9008	43626-43627	.	denotes	.
T9009	43627-43845	sentence	denotes	Moreover, through activation of Snail gene expression, TGF-β2 appears to work in tandem with these other morphogens to down-regulate E-cadherin levels, which contributes to the activation of proliferative circuitries.
T9010	43628-43636	RB	denotes	Moreover
T9012	43636-43638	,	denotes	,
T9013	43638-43645	IN	denotes	through
T9014	43646-43656	NN	denotes	activation
T9015	43657-43659	IN	denotes	of
T9016	43660-43665	NN	denotes	Snail
T9018	43666-43670	NN	denotes	gene
T9017	43671-43681	NN	denotes	expression
T9019	43681-43683	,	denotes	,
T9020	43683-43686	NN	denotes	TGF
T9022	43686-43687	HYPH	denotes	-
T9021	43687-43689	NN	denotes	β2
T9011	43690-43697	VBZ	denotes	appears
T9023	43698-43700	TO	denotes	to
T9024	43701-43705	VB	denotes	work
T9025	43706-43708	IN	denotes	in
T9026	43709-43715	NN	denotes	tandem
T9027	43716-43720	IN	denotes	with
T9028	43721-43726	DT	denotes	these
T9030	43727-43732	JJ	denotes	other
T9029	43733-43743	NNS	denotes	morphogens
T9031	43744-43746	TO	denotes	to
T9033	43747-43751	RB	denotes	down
T9034	43751-43752	HYPH	denotes	-
T9032	43752-43760	VB	denotes	regulate
T9035	43761-43762	NN	denotes	E
T9037	43762-43763	HYPH	denotes	-
T9036	43763-43771	NN	denotes	cadherin
T9038	43772-43778	NNS	denotes	levels
T9039	43778-43780	,	denotes	,
T9040	43780-43785	WDT	denotes	which
T9041	43786-43797	VBZ	denotes	contributes
T9042	43798-43800	IN	denotes	to
T9043	43801-43804	DT	denotes	the
T9044	43805-43815	NN	denotes	activation
T9045	43816-43818	IN	denotes	of
T9046	43819-43832	JJ	denotes	proliferative
T9047	43833-43844	NNS	denotes	circuitries
T9048	43844-43845	.	denotes	.
T9102	43858-43864	IN	denotes	During
T9104	43865-43872	NN	denotes	budding
T9105	43873-43886	NN	denotes	morphogenesis
T9106	43886-43888	,	denotes	,
T9107	43888-43900	VBG	denotes	intersecting
T9109	43901-43910	NN	denotes	signaling
T9108	43911-43919	NNS	denotes	networks
T9110	43920-43924	IN	denotes	from
T9111	43925-43928	DT	denotes	the
T9112	43929-43939	NN	denotes	epithelium
T9113	43940-43943	CC	denotes	and
T9114	43944-43954	NN	denotes	mesenchyme
T9103	43955-43961	VBP	denotes	govern
T9115	43962-43977	JJ	denotes	transcriptional
T9117	43977-43979	,	denotes	,
T9118	43979-43987	JJ	denotes	adhesive
T9119	43987-43989	,	denotes	,
T9120	43989-43997	NN	denotes	polarity
T9121	43997-43999	,	denotes	,
T9122	43999-44002	CC	denotes	and
T9123	44003-44011	NN	denotes	motility
T9116	44012-44020	NNS	denotes	programs
T9124	44021-44023	IN	denotes	in
T9125	44024-44029	DT	denotes	these
T9127	44030-44036	JJ	denotes	select
T9126	44037-44043	NNS	denotes	groups
T9128	44044-44046	IN	denotes	of
T9129	44047-44052	NNS	denotes	cells
T9130	44052-44053	.	denotes	.
T9131	44053-44174	sentence	denotes	The dynamic nuclear and cytosolic changes that take place during this time form the cornerstone for organ morphogenesis.
T9132	44054-44057	DT	denotes	The
T9134	44058-44065	JJ	denotes	dynamic
T9135	44066-44073	JJ	denotes	nuclear
T9136	44074-44077	CC	denotes	and
T9137	44078-44087	JJ	denotes	cytosolic
T9133	44088-44095	NNS	denotes	changes
T9139	44096-44100	WDT	denotes	that
T9140	44101-44105	VBP	denotes	take
T9141	44106-44111	NN	denotes	place
T9142	44112-44118	IN	denotes	during
T9143	44119-44123	DT	denotes	this
T9144	44124-44128	NN	denotes	time
T9138	44129-44133	VBP	denotes	form
T9145	44134-44137	DT	denotes	the
T9146	44138-44149	NN	denotes	cornerstone
T9147	44150-44153	IN	denotes	for
T9148	44154-44159	NN	denotes	organ
T9149	44160-44173	NN	denotes	morphogenesis
T9150	44173-44174	.	denotes	.
T9151	44174-44495	sentence	denotes	Two major challenges in understanding the mechanisms underlying a particular budding process are to order the temporal sequence of external cues involved and then to dissect how the cells of the developing bud translate these signals into the downstream events of cellular remodeling, proliferation, and differentiation.
T9152	44175-44178	CD	denotes	Two
T9154	44179-44184	JJ	denotes	major
T9153	44185-44195	NNS	denotes	challenges
T9156	44196-44198	IN	denotes	in
T9157	44199-44212	VBG	denotes	understanding
T9158	44213-44216	DT	denotes	the
T9159	44217-44227	NNS	denotes	mechanisms
T9160	44228-44238	VBG	denotes	underlying
T9161	44239-44240	DT	denotes	a
T9163	44241-44251	JJ	denotes	particular
T9164	44252-44259	NN	denotes	budding
T9162	44260-44267	NN	denotes	process
T9155	44268-44271	VBP	denotes	are
T9165	44272-44274	TO	denotes	to
T9166	44275-44280	VB	denotes	order
T9167	44281-44284	DT	denotes	the
T9169	44285-44293	JJ	denotes	temporal
T9168	44294-44302	NN	denotes	sequence
T9170	44303-44305	IN	denotes	of
T9171	44306-44314	JJ	denotes	external
T9172	44315-44319	NNS	denotes	cues
T9173	44320-44328	VBN	denotes	involved
T9174	44329-44332	CC	denotes	and
T9175	44333-44337	RB	denotes	then
T9177	44338-44340	TO	denotes	to
T9176	44341-44348	VB	denotes	dissect
T9178	44349-44352	WRB	denotes	how
T9180	44353-44356	DT	denotes	the
T9181	44357-44362	NNS	denotes	cells
T9182	44363-44365	IN	denotes	of
T9183	44366-44369	DT	denotes	the
T9185	44370-44380	VBG	denotes	developing
T9184	44381-44384	NN	denotes	bud
T9179	44385-44394	VB	denotes	translate
T9186	44395-44400	DT	denotes	these
T9187	44401-44408	NNS	denotes	signals
T9188	44409-44413	IN	denotes	into
T9189	44414-44417	DT	denotes	the
T9191	44418-44428	JJ	denotes	downstream
T9190	44429-44435	NNS	denotes	events
T9192	44436-44438	IN	denotes	of
T9193	44439-44447	JJ	denotes	cellular
T9194	44448-44458	NN	denotes	remodeling
T9195	44458-44460	,	denotes	,
T9196	44460-44473	NN	denotes	proliferation
T9197	44473-44475	,	denotes	,
T9198	44475-44478	CC	denotes	and
T9199	44479-44494	NN	denotes	differentiation
T9200	44494-44495	.	denotes	.
T9201	44495-44619	sentence	denotes	Our studies here provide some insights into how these events are orchestrated during hair bud formation in developing skin.
T9202	44496-44499	PRP$	denotes	Our
T9203	44500-44507	NNS	denotes	studies
T9205	44508-44512	RB	denotes	here
T9204	44513-44520	VBP	denotes	provide
T9206	44521-44525	DT	denotes	some
T9207	44526-44534	NNS	denotes	insights
T9208	44535-44539	IN	denotes	into
T9209	44540-44543	WRB	denotes	how
T9211	44544-44549	DT	denotes	these
T9212	44550-44556	NNS	denotes	events
T9213	44557-44560	VBP	denotes	are
T9210	44561-44573	VBN	denotes	orchestrated
T9214	44574-44580	IN	denotes	during
T9215	44581-44585	NN	denotes	hair
T9216	44586-44589	NN	denotes	bud
T9217	44590-44599	NN	denotes	formation
T9218	44600-44602	IN	denotes	in
T9219	44603-44613	VBG	denotes	developing
T9220	44614-44618	NN	denotes	skin
T9221	44618-44619	.	denotes	.
T9653	44621-44630	NN	denotes	Signaling
T9654	44631-44637	IN	denotes	during
T9655	44638-44643	JJ	denotes	Early
T9657	44644-44648	NN	denotes	Hair
T9658	44649-44657	NN	denotes	Follicle
T9656	44658-44671	NN	denotes	Morphogenesis
T9659	44671-44997	sentence	denotes	Recent studies on hair bud morphogenesis suggest that Wnt signals likely from the epithelium and BMP inhibitory signals from the underlying mesenchyme converge to produce an active transcription factor complex involving β-catenin and LEF-1, which in turn plays a key role in specifying the hair follicle fate [4,29,30,36,37].
T9660	44672-44678	JJ	denotes	Recent
T9661	44679-44686	NNS	denotes	studies
T9663	44687-44689	IN	denotes	on
T9664	44690-44694	NN	denotes	hair
T9665	44695-44698	NN	denotes	bud
T9666	44699-44712	NN	denotes	morphogenesis
T9662	44713-44720	VBP	denotes	suggest
T9667	44721-44725	IN	denotes	that
T9669	44726-44729	NN	denotes	Wnt
T9670	44730-44737	NNS	denotes	signals
T9671	44738-44744	RB	denotes	likely
T9672	44745-44749	IN	denotes	from
T9673	44750-44753	DT	denotes	the
T9674	44754-44764	NN	denotes	epithelium
T9675	44765-44768	CC	denotes	and
T9676	44769-44772	NN	denotes	BMP
T9678	44773-44783	JJ	denotes	inhibitory
T9677	44784-44791	NNS	denotes	signals
T9679	44792-44796	IN	denotes	from
T9680	44797-44800	DT	denotes	the
T9682	44801-44811	VBG	denotes	underlying
T9681	44812-44822	NN	denotes	mesenchyme
T9668	44823-44831	VBP	denotes	converge
T9683	44832-44834	TO	denotes	to
T9684	44835-44842	VB	denotes	produce
T9685	44843-44845	DT	denotes	an
T9687	44846-44852	JJ	denotes	active
T9688	44853-44866	NN	denotes	transcription
T9689	44867-44873	NN	denotes	factor
T9686	44874-44881	NN	denotes	complex
T9690	44882-44891	VBG	denotes	involving
T9691	44892-44893	NN	denotes	β
T9693	44893-44894	HYPH	denotes	-
T9692	44894-44901	NN	denotes	catenin
T9694	44902-44905	CC	denotes	and
T9695	44906-44909	NN	denotes	LEF
T9696	44909-44910	HYPH	denotes	-
T9697	44910-44911	CD	denotes	1
T9698	44911-44913	,	denotes	,
T9699	44913-44918	WDT	denotes	which
T9701	44919-44921	IN	denotes	in
T9702	44922-44926	NN	denotes	turn
T9700	44927-44932	VBZ	denotes	plays
T9703	44933-44934	DT	denotes	a
T9705	44935-44938	JJ	denotes	key
T9704	44939-44943	NN	denotes	role
T9706	44944-44946	IN	denotes	in
T9707	44947-44957	VBG	denotes	specifying
T9708	44958-44961	DT	denotes	the
T9710	44962-44966	NN	denotes	hair
T9711	44967-44975	NN	denotes	follicle
T9709	44976-44980	NN	denotes	fate
T9712	44981-44982	-LRB-	denotes	[
T9714	44982-44983	CD	denotes	4
T9715	44983-44984	,	denotes	,
T9716	44984-44986	CD	denotes	29
T9717	44986-44987	,	denotes	,
T9718	44987-44989	CD	denotes	30
T9719	44989-44990	,	denotes	,
T9720	44990-44992	CD	denotes	36
T9721	44992-44993	,	denotes	,
T9713	44993-44995	CD	denotes	37
T9722	44995-44996	-RRB-	denotes	]
T9723	44996-44997	.	denotes	.
T9724	44997-45256	sentence	denotes	Sonic hedgehog (Shh) and TGF-β2 signaling also play essential roles in follicle morphogenesis, but in contrast to β-catenin null skin, in which follicle invaginations are absent [30], some hair buds still form in the absence of LEF-1, Shh, or TGF-β2 [32,38].
T9725	44998-45003	JJ	denotes	Sonic
T9726	45004-45012	NN	denotes	hedgehog
T9728	45013-45014	-LRB-	denotes	(
T9729	45014-45017	NN	denotes	Shh
T9730	45017-45018	-RRB-	denotes	)
T9731	45019-45022	CC	denotes	and
T9732	45023-45026	NN	denotes	TGF
T9734	45026-45027	HYPH	denotes	-
T9733	45027-45029	NN	denotes	β2
T9735	45030-45039	NN	denotes	signaling
T9736	45040-45044	RB	denotes	also
T9727	45045-45049	VBP	denotes	play
T9737	45050-45059	JJ	denotes	essential
T9738	45060-45065	NNS	denotes	roles
T9739	45066-45068	IN	denotes	in
T9740	45069-45077	NN	denotes	follicle
T9741	45078-45091	NN	denotes	morphogenesis
T9742	45091-45093	,	denotes	,
T9743	45093-45096	CC	denotes	but
T9744	45097-45099	IN	denotes	in
T9746	45100-45108	NN	denotes	contrast
T9747	45109-45111	IN	denotes	to
T9748	45112-45113	NN	denotes	β
T9750	45113-45114	HYPH	denotes	-
T9749	45114-45121	NN	denotes	catenin
T9751	45122-45126	JJ	denotes	null
T9752	45127-45131	NN	denotes	skin
T9753	45131-45133	,	denotes	,
T9754	45133-45135	IN	denotes	in
T9756	45136-45141	WDT	denotes	which
T9757	45142-45150	NN	denotes	follicle
T9758	45151-45164	NNS	denotes	invaginations
T9755	45165-45168	VBP	denotes	are
T9759	45169-45175	JJ	denotes	absent
T9760	45176-45177	-LRB-	denotes	[
T9761	45177-45179	CD	denotes	30
T9762	45179-45180	-RRB-	denotes	]
T9763	45180-45182	,	denotes	,
T9764	45182-45186	DT	denotes	some
T9766	45187-45191	NN	denotes	hair
T9765	45192-45196	NNS	denotes	buds
T9767	45197-45202	RB	denotes	still
T9745	45203-45207	VBP	denotes	form
T9768	45208-45210	IN	denotes	in
T9769	45211-45214	DT	denotes	the
T9770	45215-45222	NN	denotes	absence
T9771	45223-45225	IN	denotes	of
T9772	45226-45229	NN	denotes	LEF
T9773	45229-45230	HYPH	denotes	-
T9774	45230-45231	CD	denotes	1
T9775	45231-45233	,	denotes	,
T9776	45233-45236	NN	denotes	Shh
T9777	45236-45238	,	denotes	,
T9778	45238-45240	CC	denotes	or
T9779	45241-45244	NN	denotes	TGF
T9781	45244-45245	HYPH	denotes	-
T9780	45245-45247	NN	denotes	β2
T9782	45248-45249	-LRB-	denotes	[
T9784	45249-45251	CD	denotes	32
T9785	45251-45252	,	denotes	,
T9783	45252-45254	CD	denotes	38
T9786	45254-45255	-RRB-	denotes	]
T9787	45255-45256	.	denotes	.
T9788	45256-45439	sentence	denotes	These likely reflect the first wave of follicle (i.e., guard hair) morphogenesis, which accounts for a small number (fewer than 5%) of hairs and is under distinct regulatory control.
T9789	45257-45262	DT	denotes	These
T9791	45263-45269	RB	denotes	likely
T9790	45270-45277	VBP	denotes	reflect
T9792	45278-45281	DT	denotes	the
T9794	45282-45287	JJ	denotes	first
T9793	45288-45292	NN	denotes	wave
T9795	45293-45295	IN	denotes	of
T9796	45296-45304	NN	denotes	follicle
T9798	45305-45306	-LRB-	denotes	(
T9800	45306-45310	FW	denotes	i.e.
T9801	45310-45312	,	denotes	,
T9802	45312-45317	NN	denotes	guard
T9799	45318-45322	NN	denotes	hair
T9803	45322-45323	-RRB-	denotes	)
T9797	45324-45337	NN	denotes	morphogenesis
T9804	45337-45339	,	denotes	,
T9805	45339-45344	WDT	denotes	which
T9806	45345-45353	VBZ	denotes	accounts
T9807	45354-45357	IN	denotes	for
T9808	45358-45359	DT	denotes	a
T9810	45360-45365	JJ	denotes	small
T9809	45366-45372	NN	denotes	number
T9811	45373-45374	-LRB-	denotes	(
T9812	45374-45379	JJR	denotes	fewer
T9814	45380-45384	IN	denotes	than
T9813	45385-45386	CD	denotes	5
T9815	45386-45387	NN	denotes	%
T9816	45387-45388	-RRB-	denotes	)
T9817	45389-45391	IN	denotes	of
T9818	45392-45397	NNS	denotes	hairs
T9819	45398-45401	CC	denotes	and
T9820	45402-45404	VBZ	denotes	is
T9821	45405-45410	IN	denotes	under
T9822	45411-45419	JJ	denotes	distinct
T9824	45420-45430	JJ	denotes	regulatory
T9823	45431-45438	NN	denotes	control
T9825	45438-45439	.	denotes	.
T9826	45439-45603	sentence	denotes	Guard hairs form in the absence of LEF-1 and TGF-β2, and we have found that they also fail to express Snail at the budding stage of development (unpublished data).
T9827	45440-45445	NN	denotes	Guard
T9828	45446-45451	NNS	denotes	hairs
T9829	45452-45456	VBP	denotes	form
T9830	45457-45459	IN	denotes	in
T9831	45460-45463	DT	denotes	the
T9832	45464-45471	NN	denotes	absence
T9833	45472-45474	IN	denotes	of
T9834	45475-45478	NN	denotes	LEF
T9835	45478-45479	HYPH	denotes	-
T9836	45479-45480	CD	denotes	1
T9837	45481-45484	CC	denotes	and
T9838	45485-45488	NN	denotes	TGF
T9840	45488-45489	HYPH	denotes	-
T9839	45489-45491	NN	denotes	β2
T9841	45491-45493	,	denotes	,
T9842	45493-45496	CC	denotes	and
T9843	45497-45499	PRP	denotes	we
T9845	45500-45504	VBP	denotes	have
T9844	45505-45510	VBN	denotes	found
T9846	45511-45515	IN	denotes	that
T9848	45516-45520	PRP	denotes	they
T9849	45521-45525	RB	denotes	also
T9847	45526-45530	VBP	denotes	fail
T9850	45531-45533	TO	denotes	to
T9851	45534-45541	VB	denotes	express
T9852	45542-45547	NN	denotes	Snail
T9853	45548-45550	IN	denotes	at
T9854	45551-45554	DT	denotes	the
T9856	45555-45562	NN	denotes	budding
T9855	45563-45568	NN	denotes	stage
T9857	45569-45571	IN	denotes	of
T9858	45572-45583	NN	denotes	development
T9859	45584-45585	-LRB-	denotes	(
T9861	45585-45596	JJ	denotes	unpublished
T9860	45597-45601	NNS	denotes	data
T9862	45601-45602	-RRB-	denotes	)
T9863	45602-45603	.	denotes	.
T9864	45603-45672	sentence	denotes	How E-cadherin is regulated in guard hairs remains to be determined.
T9865	45604-45607	WRB	denotes	How
T9867	45608-45609	NN	denotes	E
T9869	45609-45610	HYPH	denotes	-
T9868	45610-45618	NN	denotes	cadherin
T9870	45619-45621	VBZ	denotes	is
T9866	45622-45631	VBN	denotes	regulated
T9872	45632-45634	IN	denotes	in
T9873	45635-45640	NN	denotes	guard
T9874	45641-45646	NNS	denotes	hairs
T9871	45647-45654	VBZ	denotes	remains
T9875	45655-45657	TO	denotes	to
T9877	45658-45660	VB	denotes	be
T9876	45661-45671	VBN	denotes	determined
T9878	45671-45672	.	denotes	.
T9879	45672-45906	sentence	denotes	Several candidates include other Snail family members such as Slug or twist, or alternatively, transcription factors involving β-catenin and a different member of the LEF-1/TCF/Sry-type HMG box (commonly known as SOX) family [39,40].
T9880	45673-45680	JJ	denotes	Several
T9881	45681-45691	NNS	denotes	candidates
T9882	45692-45699	VBP	denotes	include
T9883	45700-45705	JJ	denotes	other
T9885	45706-45711	NN	denotes	Snail
T9886	45712-45718	NN	denotes	family
T9884	45719-45726	NNS	denotes	members
T9887	45727-45731	JJ	denotes	such
T9888	45732-45734	IN	denotes	as
T9889	45735-45739	NN	denotes	Slug
T9890	45740-45742	CC	denotes	or
T9891	45743-45748	NN	denotes	twist
T9892	45748-45750	,	denotes	,
T9893	45750-45752	CC	denotes	or
T9894	45753-45766	RB	denotes	alternatively
T9896	45766-45768	,	denotes	,
T9897	45768-45781	NN	denotes	transcription
T9895	45782-45789	NNS	denotes	factors
T9898	45790-45799	VBG	denotes	involving
T9899	45800-45801	NN	denotes	β
T9901	45801-45802	HYPH	denotes	-
T9900	45802-45809	NN	denotes	catenin
T9902	45810-45813	CC	denotes	and
T9903	45814-45815	DT	denotes	a
T9905	45816-45825	JJ	denotes	different
T9904	45826-45832	NN	denotes	member
T9906	45833-45835	IN	denotes	of
T9907	45836-45839	DT	denotes	the
T9909	45840-45843	NN	denotes	LEF
T9911	45843-45844	HYPH	denotes	-
T9912	45844-45845	CD	denotes	1
T9913	45845-45846	HYPH	denotes	/
T9914	45846-45849	NN	denotes	TCF
T9915	45849-45850	HYPH	denotes	/
T9916	45850-45853	NN	denotes	Sry
T9918	45853-45854	HYPH	denotes	-
T9917	45854-45858	NN	denotes	type
T9919	45859-45862	NN	denotes	HMG
T9910	45863-45866	NN	denotes	box
T9920	45867-45868	-LRB-	denotes	(
T9922	45868-45876	RB	denotes	commonly
T9921	45877-45882	VBN	denotes	known
T9923	45883-45885	IN	denotes	as
T9924	45886-45889	NN	denotes	SOX
T9925	45889-45890	-RRB-	denotes	)
T9908	45891-45897	NN	denotes	family
T9926	45898-45899	-LRB-	denotes	[
T9928	45899-45901	CD	denotes	39
T9929	45901-45902	,	denotes	,
T9927	45902-45904	CD	denotes	40
T9930	45904-45905	-RRB-	denotes	]
T9931	45905-45906	.	denotes	.
T9932	45906-46049	sentence	denotes	Further investigation will be required to determine whether the signaling pathway we have elucidated here is a theme with multiple variations.
T9933	45907-45914	JJ	denotes	Further
T9934	45915-45928	NN	denotes	investigation
T9936	45929-45933	MD	denotes	will
T9937	45934-45936	VB	denotes	be
T9935	45937-45945	VBN	denotes	required
T9938	45946-45948	TO	denotes	to
T9939	45949-45958	VB	denotes	determine
T9940	45959-45966	IN	denotes	whether
T9942	45967-45970	DT	denotes	the
T9944	45971-45980	NN	denotes	signaling
T9943	45981-45988	NN	denotes	pathway
T9945	45989-45991	PRP	denotes	we
T9947	45992-45996	VBP	denotes	have
T9946	45997-46007	VBN	denotes	elucidated
T9948	46008-46012	RB	denotes	here
T9941	46013-46015	VBZ	denotes	is
T9949	46016-46017	DT	denotes	a
T9950	46018-46023	NN	denotes	theme
T9951	46024-46028	IN	denotes	with
T9952	46029-46037	JJ	denotes	multiple
T9953	46038-46048	NNS	denotes	variations
T9954	46048-46049	.	denotes	.
T9955	46049-46131	sentence	denotes	TGF-βs are known to promote withdrawal of keratinocytes from the cell cycle [41].
T9956	46050-46053	NN	denotes	TGF
T9958	46053-46054	HYPH	denotes	-
T9957	46054-46056	NNS	denotes	βs
T9960	46057-46060	VBP	denotes	are
T9959	46061-46066	VBN	denotes	known
T9961	46067-46069	TO	denotes	to
T9962	46070-46077	VB	denotes	promote
T9963	46078-46088	NN	denotes	withdrawal
T9964	46089-46091	IN	denotes	of
T9965	46092-46105	NNS	denotes	keratinocytes
T9966	46106-46110	IN	denotes	from
T9967	46111-46114	DT	denotes	the
T9969	46115-46119	NN	denotes	cell
T9968	46120-46125	NN	denotes	cycle
T9970	46126-46127	-LRB-	denotes	[
T9971	46127-46129	CD	denotes	41
T9972	46129-46130	-RRB-	denotes	]
T9973	46130-46131	.	denotes	.
T9974	46131-46413	sentence	denotes	Hence, when TGF-β2 protein was detected at the transition between the growing and destructive phases of the adult hair cycle, research initially and naturally focused on a role for this family member in cessation of growth and/or triggering apoptosis ([42] and references therein).
T9975	46132-46137	RB	denotes	Hence
T9977	46137-46139	,	denotes	,
T9978	46139-46143	WRB	denotes	when
T9980	46144-46147	NN	denotes	TGF
T9982	46147-46148	HYPH	denotes	-
T9981	46148-46150	NN	denotes	β2
T9983	46151-46158	NN	denotes	protein
T9984	46159-46162	VBD	denotes	was
T9979	46163-46171	VBN	denotes	detected
T9985	46172-46174	IN	denotes	at
T9986	46175-46178	DT	denotes	the
T9987	46179-46189	NN	denotes	transition
T9988	46190-46197	IN	denotes	between
T9989	46198-46201	DT	denotes	the
T9991	46202-46209	NN	denotes	growing
T9993	46210-46213	CC	denotes	and
T9992	46214-46225	JJ	denotes	destructive
T9990	46226-46232	NNS	denotes	phases
T9994	46233-46235	IN	denotes	of
T9995	46236-46239	DT	denotes	the
T9997	46240-46245	JJ	denotes	adult
T9998	46246-46250	NN	denotes	hair
T9996	46251-46256	NN	denotes	cycle
T9999	46256-46258	,	denotes	,
T10000	46258-46266	NN	denotes	research
T10001	46267-46276	RB	denotes	initially
T10002	46277-46280	CC	denotes	and
T10003	46281-46290	RB	denotes	naturally
T9976	46291-46298	VBD	denotes	focused
T10004	46299-46301	IN	denotes	on
T10005	46302-46303	DT	denotes	a
T10006	46304-46308	NN	denotes	role
T10007	46309-46312	IN	denotes	for
T10008	46313-46317	DT	denotes	this
T10010	46318-46324	NN	denotes	family
T10009	46325-46331	NN	denotes	member
T10011	46332-46334	IN	denotes	in
T10012	46335-46344	NN	denotes	cessation
T10013	46345-46347	IN	denotes	of
T10014	46348-46354	NN	denotes	growth
T10015	46355-46358	CC	denotes	and
T10016	46358-46359	HYPH	denotes	/
T10017	46359-46361	CC	denotes	or
T10018	46362-46372	VBG	denotes	triggering
T10019	46373-46382	NN	denotes	apoptosis
T10020	46383-46384	-LRB-	denotes	(
T10022	46384-46385	-LRB-	denotes	[
T10021	46385-46387	CD	denotes	42
T10023	46387-46388	-RRB-	denotes	]
T10024	46389-46392	CC	denotes	and
T10025	46393-46403	NNS	denotes	references
T10026	46404-46411	RB	denotes	therein
T10027	46411-46412	-RRB-	denotes	)
T10028	46412-46413	.	denotes	.
T10029	46413-46604	sentence	denotes	However, in contrast to TGF-β1-null skin, which exhibits an extended growing phase of postnatal hair follicles, TGF-β2-null skin displays an embryonic block in follicle bud progression [32].
T10030	46414-46421	RB	denotes	However
T10032	46421-46423	,	denotes	,
T10033	46423-46425	IN	denotes	in
T10034	46426-46434	NN	denotes	contrast
T10035	46435-46437	IN	denotes	to
T10036	46438-46441	NN	denotes	TGF
T10038	46441-46442	HYPH	denotes	-
T10037	46442-46444	NN	denotes	β1
T10040	46444-46445	HYPH	denotes	-
T10039	46445-46449	JJ	denotes	null
T10041	46450-46454	NN	denotes	skin
T10042	46454-46456	,	denotes	,
T10043	46456-46461	WDT	denotes	which
T10044	46462-46470	VBZ	denotes	exhibits
T10045	46471-46473	DT	denotes	an
T10047	46474-46482	JJ	denotes	extended
T10048	46483-46490	NN	denotes	growing
T10046	46491-46496	NN	denotes	phase
T10049	46497-46499	IN	denotes	of
T10050	46500-46509	JJ	denotes	postnatal
T10052	46510-46514	NN	denotes	hair
T10051	46515-46524	NNS	denotes	follicles
T10053	46524-46526	,	denotes	,
T10054	46526-46529	NN	denotes	TGF
T10056	46529-46530	HYPH	denotes	-
T10055	46530-46532	NN	denotes	β2
T10058	46532-46533	HYPH	denotes	-
T10057	46533-46537	JJ	denotes	null
T10059	46538-46542	NN	denotes	skin
T10031	46543-46551	VBZ	denotes	displays
T10060	46552-46554	DT	denotes	an
T10062	46555-46564	JJ	denotes	embryonic
T10061	46565-46570	NN	denotes	block
T10063	46571-46573	IN	denotes	in
T10064	46574-46582	NN	denotes	follicle
T10065	46583-46586	NN	denotes	bud
T10066	46587-46598	NN	denotes	progression
T10067	46599-46600	-LRB-	denotes	[
T10068	46600-46602	CD	denotes	32
T10069	46602-46603	-RRB-	denotes	]
T10070	46603-46604	.	denotes	.
T10071	46604-46875	sentence	denotes	Although this phenotype is consistent with TGF-β2's embryonic expression patterns [33], about 50% of TGF-β2 null buds appear unable to progress to the down-growth phase, a feature that cannot be explained readily on the basis of previously established effects of TGF-βs.
T10072	46605-46613	IN	denotes	Although
T10074	46614-46618	DT	denotes	this
T10075	46619-46628	NN	denotes	phenotype
T10073	46629-46631	VBZ	denotes	is
T10077	46632-46642	JJ	denotes	consistent
T10078	46643-46647	IN	denotes	with
T10079	46648-46651	NN	denotes	TGF
T10081	46651-46652	HYPH	denotes	-
T10080	46652-46654	NN	denotes	β2
T10083	46654-46656	POS	denotes	's
T10084	46657-46666	JJ	denotes	embryonic
T10085	46667-46677	NN	denotes	expression
T10082	46678-46686	NNS	denotes	patterns
T10086	46687-46688	-LRB-	denotes	[
T10087	46688-46690	CD	denotes	33
T10088	46690-46691	-RRB-	denotes	]
T10089	46691-46693	,	denotes	,
T10090	46693-46698	IN	denotes	about
T10091	46699-46701	CD	denotes	50
T10092	46701-46702	NN	denotes	%
T10093	46703-46705	IN	denotes	of
T10094	46706-46709	NN	denotes	TGF
T10096	46709-46710	HYPH	denotes	-
T10095	46710-46712	NN	denotes	β2
T10097	46713-46717	JJ	denotes	null
T10098	46718-46722	NNS	denotes	buds
T10076	46723-46729	VBP	denotes	appear
T10099	46730-46736	JJ	denotes	unable
T10100	46737-46739	TO	denotes	to
T10101	46740-46748	VB	denotes	progress
T10102	46749-46751	IN	denotes	to
T10103	46752-46755	DT	denotes	the
T10105	46756-46760	JJ	denotes	down
T10107	46760-46761	HYPH	denotes	-
T10106	46761-46767	NN	denotes	growth
T10104	46768-46773	NN	denotes	phase
T10108	46773-46775	,	denotes	,
T10109	46775-46776	DT	denotes	a
T10110	46777-46784	NN	denotes	feature
T10111	46785-46789	WDT	denotes	that
T10113	46790-46793	MD	denotes	can
T10114	46793-46796	RB	denotes	not
T10115	46797-46799	VB	denotes	be
T10112	46800-46809	VBN	denotes	explained
T10116	46810-46817	RB	denotes	readily
T10117	46818-46820	IN	denotes	on
T10118	46821-46824	DT	denotes	the
T10119	46825-46830	NN	denotes	basis
T10120	46831-46833	IN	denotes	of
T10121	46834-46844	RB	denotes	previously
T10122	46845-46856	VBN	denotes	established
T10123	46857-46864	NNS	denotes	effects
T10124	46865-46867	IN	denotes	of
T10125	46868-46871	NN	denotes	TGF
T10127	46871-46872	HYPH	denotes	-
T10126	46872-46874	NNS	denotes	βs
T10128	46874-46875	.	denotes	.
T10129	46875-47150	sentence	denotes	Our finding that TGF-β2 is upstream from Ki67 expression and MAPK activation lends further support to the notion that hair follicle keratinocytes at this early stage of development react to TGF-β2 signaling in a fashion opposite to that typically expected for TGF-β factors.
T10130	46876-46879	PRP$	denotes	Our
T10131	46880-46887	NN	denotes	finding
T10133	46888-46892	IN	denotes	that
T10135	46893-46896	NN	denotes	TGF
T10137	46896-46897	HYPH	denotes	-
T10136	46897-46899	NN	denotes	β2
T10134	46900-46902	VBZ	denotes	is
T10138	46903-46911	RB	denotes	upstream
T10139	46912-46916	IN	denotes	from
T10140	46917-46921	NN	denotes	Ki67
T10141	46922-46932	NN	denotes	expression
T10142	46933-46936	CC	denotes	and
T10143	46937-46941	NN	denotes	MAPK
T10144	46942-46952	NN	denotes	activation
T10132	46953-46958	VBZ	denotes	lends
T10145	46959-46966	JJ	denotes	further
T10146	46967-46974	NN	denotes	support
T10147	46975-46977	IN	denotes	to
T10148	46978-46981	DT	denotes	the
T10149	46982-46988	NN	denotes	notion
T10150	46989-46993	IN	denotes	that
T10152	46994-46998	NN	denotes	hair
T10153	46999-47007	NN	denotes	follicle
T10154	47008-47021	NNS	denotes	keratinocytes
T10155	47022-47024	IN	denotes	at
T10156	47025-47029	DT	denotes	this
T10158	47030-47035	JJ	denotes	early
T10157	47036-47041	NN	denotes	stage
T10159	47042-47044	IN	denotes	of
T10160	47045-47056	NN	denotes	development
T10151	47057-47062	VBP	denotes	react
T10161	47063-47065	IN	denotes	to
T10162	47066-47069	NN	denotes	TGF
T10164	47069-47070	HYPH	denotes	-
T10163	47070-47072	NN	denotes	β2
T10165	47073-47082	NN	denotes	signaling
T10166	47083-47085	IN	denotes	in
T10167	47086-47087	DT	denotes	a
T10168	47088-47095	NN	denotes	fashion
T10169	47096-47104	JJ	denotes	opposite
T10170	47105-47107	IN	denotes	to
T10171	47108-47112	DT	denotes	that
T10172	47113-47122	RB	denotes	typically
T10173	47123-47131	VBN	denotes	expected
T10174	47132-47135	IN	denotes	for
T10175	47136-47139	NN	denotes	TGF
T10177	47139-47140	HYPH	denotes	-
T10176	47140-47141	NN	denotes	β
T10178	47142-47149	NNS	denotes	factors
T10179	47149-47150	.	denotes	.
T10180	47150-47268	sentence	denotes	This said, based upon pSMAD2 immunohistochemistry, the immediate steps of downstream signaling appeared to be intact.
T10181	47151-47155	DT	denotes	This
T10182	47156-47160	VBN	denotes	said
T10184	47160-47162	,	denotes	,
T10185	47162-47167	VBN	denotes	based
T10186	47168-47172	IN	denotes	upon
T10187	47173-47179	NN	denotes	pSMAD2
T10188	47180-47200	NN	denotes	immunohistochemistry
T10189	47200-47202	,	denotes	,
T10190	47202-47205	DT	denotes	the
T10192	47206-47215	JJ	denotes	immediate
T10191	47216-47221	NNS	denotes	steps
T10193	47222-47224	IN	denotes	of
T10194	47225-47235	JJ	denotes	downstream
T10195	47236-47245	NN	denotes	signaling
T10183	47246-47254	VBD	denotes	appeared
T10196	47255-47257	TO	denotes	to
T10197	47258-47260	VB	denotes	be
T10198	47261-47267	JJ	denotes	intact
T10199	47267-47268	.	denotes	.
T10200	47268-47404	sentence	denotes	Thus, we surmise that the proliferative outcome is likely to be rooted in differences in the repertoire of activated SMAD target genes.
T10201	47269-47273	RB	denotes	Thus
T10203	47273-47275	,	denotes	,
T10204	47275-47277	PRP	denotes	we
T10202	47278-47285	VBP	denotes	surmise
T10205	47286-47290	IN	denotes	that
T10207	47291-47294	DT	denotes	the
T10209	47295-47308	JJ	denotes	proliferative
T10208	47309-47316	NN	denotes	outcome
T10206	47317-47319	VBZ	denotes	is
T10210	47320-47326	JJ	denotes	likely
T10211	47327-47329	TO	denotes	to
T10213	47330-47332	VB	denotes	be
T10212	47333-47339	VBN	denotes	rooted
T10214	47340-47342	IN	denotes	in
T10215	47343-47354	NNS	denotes	differences
T10216	47355-47357	IN	denotes	in
T10217	47358-47361	DT	denotes	the
T10218	47362-47372	NN	denotes	repertoire
T10219	47373-47375	IN	denotes	of
T10220	47376-47385	VBN	denotes	activated
T10222	47386-47390	NN	denotes	SMAD
T10223	47391-47397	NN	denotes	target
T10221	47398-47403	NNS	denotes	genes
T10224	47403-47404	.	denotes	.
T10225	47404-47669	sentence	denotes	In this regard, the positive effects of TGF-β2 on proliferation within the hair bud may be more analogous to what has been seen in progression of squamous cell carcinoma to metastatic carcinoma [43] rather than that typically observed for keratinocytes [44,45,46].
T10226	47405-47407	IN	denotes	In
T10228	47408-47412	DT	denotes	this
T10229	47413-47419	NN	denotes	regard
T10230	47419-47421	,	denotes	,
T10231	47421-47424	DT	denotes	the
T10233	47425-47433	JJ	denotes	positive
T10232	47434-47441	NNS	denotes	effects
T10234	47442-47444	IN	denotes	of
T10235	47445-47448	NN	denotes	TGF
T10237	47448-47449	HYPH	denotes	-
T10236	47449-47451	NN	denotes	β2
T10238	47452-47454	IN	denotes	on
T10239	47455-47468	NN	denotes	proliferation
T10240	47469-47475	IN	denotes	within
T10241	47476-47479	DT	denotes	the
T10243	47480-47484	NN	denotes	hair
T10242	47485-47488	NN	denotes	bud
T10244	47489-47492	MD	denotes	may
T10227	47493-47495	VB	denotes	be
T10245	47496-47500	RBR	denotes	more
T10246	47501-47510	JJ	denotes	analogous
T10247	47511-47513	IN	denotes	to
T10248	47514-47518	WP	denotes	what
T10250	47519-47522	VBZ	denotes	has
T10251	47523-47527	VBN	denotes	been
T10249	47528-47532	VBN	denotes	seen
T10252	47533-47535	IN	denotes	in
T10253	47536-47547	NN	denotes	progression
T10254	47548-47550	IN	denotes	of
T10255	47551-47559	JJ	denotes	squamous
T10256	47560-47564	NN	denotes	cell
T10257	47565-47574	NN	denotes	carcinoma
T10258	47575-47577	IN	denotes	to
T10259	47578-47588	JJ	denotes	metastatic
T10260	47589-47598	NN	denotes	carcinoma
T10261	47599-47600	-LRB-	denotes	[
T10262	47600-47602	CD	denotes	43
T10263	47602-47603	-RRB-	denotes	]
T10264	47604-47610	RB	denotes	rather
T10265	47611-47615	IN	denotes	than
T10266	47616-47620	DT	denotes	that
T10267	47621-47630	RB	denotes	typically
T10268	47631-47639	VBN	denotes	observed
T10269	47640-47643	IN	denotes	for
T10270	47644-47657	NNS	denotes	keratinocytes
T10271	47658-47659	-LRB-	denotes	[
T10273	47659-47661	CD	denotes	44
T10274	47661-47662	,	denotes	,
T10275	47662-47664	CD	denotes	45
T10276	47664-47665	,	denotes	,
T10272	47665-47667	CD	denotes	46
T10277	47667-47668	-RRB-	denotes	]
T10278	47668-47669	.	denotes	.
T10279	47669-47863	sentence	denotes	The prior identification of the Snail gene as a potential target of TGF-β signaling [15] was intriguing, given the temporal wave of Snail gene expression that occurs in the developing hair bud.
T10280	47670-47673	DT	denotes	The
T10282	47674-47679	JJ	denotes	prior
T10281	47680-47694	NN	denotes	identification
T10284	47695-47697	IN	denotes	of
T10285	47698-47701	DT	denotes	the
T10287	47702-47707	NN	denotes	Snail
T10286	47708-47712	NN	denotes	gene
T10288	47713-47715	IN	denotes	as
T10289	47716-47717	DT	denotes	a
T10291	47718-47727	JJ	denotes	potential
T10290	47728-47734	NN	denotes	target
T10292	47735-47737	IN	denotes	of
T10293	47738-47741	NN	denotes	TGF
T10295	47741-47742	HYPH	denotes	-
T10294	47742-47743	NN	denotes	β
T10296	47744-47753	NN	denotes	signaling
T10297	47754-47755	-LRB-	denotes	[
T10298	47755-47757	CD	denotes	15
T10299	47757-47758	-RRB-	denotes	]
T10283	47759-47762	VBD	denotes	was
T10300	47763-47773	JJ	denotes	intriguing
T10301	47773-47775	,	denotes	,
T10302	47775-47780	VBN	denotes	given
T10303	47781-47784	DT	denotes	the
T10305	47785-47793	JJ	denotes	temporal
T10304	47794-47798	NN	denotes	wave
T10306	47799-47801	IN	denotes	of
T10307	47802-47807	NN	denotes	Snail
T10309	47808-47812	NN	denotes	gene
T10308	47813-47823	NN	denotes	expression
T10310	47824-47828	WDT	denotes	that
T10311	47829-47835	VBZ	denotes	occurs
T10312	47836-47838	IN	denotes	in
T10313	47839-47842	DT	denotes	the
T10315	47843-47853	VBG	denotes	developing
T10316	47854-47858	NN	denotes	hair
T10314	47859-47862	NN	denotes	bud
T10317	47862-47863	.	denotes	.
T10318	47863-48034	sentence	denotes	The additional correlation between epithelial hyperproliferation and Snail transgene expression further fostered our interest in a possible link between TGF-β2 and Snail.
T10319	47864-47867	DT	denotes	The
T10321	47868-47878	JJ	denotes	additional
T10320	47879-47890	NN	denotes	correlation
T10323	47891-47898	IN	denotes	between
T10324	47899-47909	JJ	denotes	epithelial
T10325	47910-47928	NN	denotes	hyperproliferation
T10326	47929-47932	CC	denotes	and
T10327	47933-47938	NN	denotes	Snail
T10329	47939-47948	NN	denotes	transgene
T10328	47949-47959	NN	denotes	expression
T10330	47960-47967	RB	denotes	further
T10322	47968-47976	VBD	denotes	fostered
T10331	47977-47980	PRP$	denotes	our
T10332	47981-47989	NN	denotes	interest
T10333	47990-47992	IN	denotes	in
T10334	47993-47994	DT	denotes	a
T10336	47995-48003	JJ	denotes	possible
T10335	48004-48008	NN	denotes	link
T10337	48009-48016	IN	denotes	between
T10338	48017-48020	NN	denotes	TGF
T10340	48020-48021	HYPH	denotes	-
T10339	48021-48023	NN	denotes	β2
T10341	48024-48027	CC	denotes	and
T10342	48028-48033	NN	denotes	Snail
T10343	48033-48034	.	denotes	.
T10344	48034-48212	sentence	denotes	Our functional studies demonstrate that without TGF-β2, Snail expression is abolished in the mutant hair buds, and conversely, in K14-Smad2 skin, Snail is ectopically activated.
T10345	48035-48038	PRP$	denotes	Our
T10347	48039-48049	JJ	denotes	functional
T10346	48050-48057	NNS	denotes	studies
T10348	48058-48069	VBP	denotes	demonstrate
T10349	48070-48074	IN	denotes	that
T10351	48075-48082	IN	denotes	without
T10352	48083-48086	NN	denotes	TGF
T10354	48086-48087	HYPH	denotes	-
T10353	48087-48089	NN	denotes	β2
T10355	48089-48091	,	denotes	,
T10356	48091-48096	NN	denotes	Snail
T10357	48097-48107	NN	denotes	expression
T10358	48108-48110	VBZ	denotes	is
T10350	48111-48120	VBN	denotes	abolished
T10359	48121-48123	IN	denotes	in
T10360	48124-48127	DT	denotes	the
T10362	48128-48134	NN	denotes	mutant
T10363	48135-48139	NN	denotes	hair
T10361	48140-48144	NNS	denotes	buds
T10364	48144-48146	,	denotes	,
T10365	48146-48149	CC	denotes	and
T10366	48150-48160	RB	denotes	conversely
T10368	48160-48162	,	denotes	,
T10369	48162-48164	IN	denotes	in
T10370	48165-48168	NN	denotes	K14
T10372	48168-48169	HYPH	denotes	-
T10371	48169-48174	NN	denotes	Smad2
T10373	48175-48179	NN	denotes	skin
T10374	48179-48181	,	denotes	,
T10375	48181-48186	NN	denotes	Snail
T10376	48187-48189	VBZ	denotes	is
T10377	48190-48201	RB	denotes	ectopically
T10367	48202-48211	VBN	denotes	activated
T10378	48211-48212	.	denotes	.
T10379	48212-48443	sentence	denotes	Moreover, our in vitro studies indicate that even sustained TGF-β2 exposure may cause only a transient induction of Snail, offering a possible explanation as to why Snail is so briefly expressed during hair follicle morphogenesis.
T10380	48213-48221	RB	denotes	Moreover
T10382	48221-48223	,	denotes	,
T10383	48223-48226	PRP$	denotes	our
T10385	48227-48229	FW	denotes	in
T10386	48230-48235	FW	denotes	vitro
T10384	48236-48243	NNS	denotes	studies
T10381	48244-48252	VBP	denotes	indicate
T10387	48253-48257	IN	denotes	that
T10389	48258-48262	RB	denotes	even
T10391	48263-48272	JJ	denotes	sustained
T10392	48273-48276	NN	denotes	TGF
T10394	48276-48277	HYPH	denotes	-
T10393	48277-48279	NN	denotes	β2
T10390	48280-48288	NN	denotes	exposure
T10395	48289-48292	MD	denotes	may
T10388	48293-48298	VB	denotes	cause
T10396	48299-48303	RB	denotes	only
T10398	48304-48305	DT	denotes	a
T10399	48306-48315	JJ	denotes	transient
T10397	48316-48325	NN	denotes	induction
T10400	48326-48328	IN	denotes	of
T10401	48329-48334	NN	denotes	Snail
T10402	48334-48336	,	denotes	,
T10403	48336-48344	VBG	denotes	offering
T10404	48345-48346	DT	denotes	a
T10406	48347-48355	JJ	denotes	possible
T10405	48356-48367	NN	denotes	explanation
T10407	48368-48370	IN	denotes	as
T10408	48371-48373	IN	denotes	to
T10409	48374-48377	WRB	denotes	why
T10411	48378-48383	NN	denotes	Snail
T10412	48384-48386	VBZ	denotes	is
T10413	48387-48389	RB	denotes	so
T10414	48390-48397	RB	denotes	briefly
T10410	48398-48407	VBN	denotes	expressed
T10415	48408-48414	IN	denotes	during
T10416	48415-48419	NN	denotes	hair
T10417	48420-48428	NN	denotes	follicle
T10418	48429-48442	NN	denotes	morphogenesis
T10419	48442-48443	.	denotes	.
T10420	48443-48760	sentence	denotes	An additional point worth mentioning is that prolonged expression of Tg Snail in postnatal skin resulted in morphological and biochemical signs of epithelial to mesenchymal transitions (unpublished data), underscoring why transient Snail expression may be so important during normal hair follicle morphogenesis [18].
T10421	48444-48446	DT	denotes	An
T10423	48447-48457	JJ	denotes	additional
T10422	48458-48463	NN	denotes	point
T10425	48464-48469	JJ	denotes	worth
T10426	48470-48480	VBG	denotes	mentioning
T10424	48481-48483	VBZ	denotes	is
T10427	48484-48488	IN	denotes	that
T10429	48489-48498	VBN	denotes	prolonged
T10430	48499-48509	NN	denotes	expression
T10431	48510-48512	IN	denotes	of
T10432	48513-48515	NN	denotes	Tg
T10433	48516-48521	NN	denotes	Snail
T10434	48522-48524	IN	denotes	in
T10435	48525-48534	JJ	denotes	postnatal
T10436	48535-48539	NN	denotes	skin
T10428	48540-48548	VBD	denotes	resulted
T10437	48549-48551	IN	denotes	in
T10438	48552-48565	JJ	denotes	morphological
T10440	48566-48569	CC	denotes	and
T10441	48570-48581	JJ	denotes	biochemical
T10439	48582-48587	NNS	denotes	signs
T10442	48588-48590	IN	denotes	of
T10443	48591-48601	JJ	denotes	epithelial
T10445	48602-48604	IN	denotes	to
T10446	48605-48616	JJ	denotes	mesenchymal
T10444	48617-48628	NNS	denotes	transitions
T10447	48629-48630	-LRB-	denotes	(
T10449	48630-48641	JJ	denotes	unpublished
T10448	48642-48646	NNS	denotes	data
T10450	48646-48647	-RRB-	denotes	)
T10451	48647-48649	,	denotes	,
T10452	48649-48661	VBG	denotes	underscoring
T10453	48662-48665	WRB	denotes	why
T10455	48666-48675	JJ	denotes	transient
T10457	48676-48681	NN	denotes	Snail
T10456	48682-48692	NN	denotes	expression
T10458	48693-48696	MD	denotes	may
T10454	48697-48699	VB	denotes	be
T10459	48700-48702	RB	denotes	so
T10460	48703-48712	JJ	denotes	important
T10461	48713-48719	IN	denotes	during
T10462	48720-48726	JJ	denotes	normal
T10464	48727-48731	NN	denotes	hair
T10465	48732-48740	NN	denotes	follicle
T10463	48741-48754	NN	denotes	morphogenesis
T10466	48755-48756	-LRB-	denotes	[
T10467	48756-48758	CD	denotes	18
T10468	48758-48759	-RRB-	denotes	]
T10469	48759-48760	.	denotes	.
T10470	48760-48893	sentence	denotes	At first glance, the sparsity in hair coat of K14-Snail Tg mice seemed indicative of a defect in follicle formation (see Figure 2A).
T10471	48761-48763	IN	denotes	At
T10473	48764-48769	JJ	denotes	first
T10474	48770-48776	NN	denotes	glance
T10475	48776-48778	,	denotes	,
T10476	48778-48781	DT	denotes	the
T10477	48782-48790	NN	denotes	sparsity
T10478	48791-48793	IN	denotes	in
T10479	48794-48798	NN	denotes	hair
T10480	48799-48803	NN	denotes	coat
T10481	48804-48806	IN	denotes	of
T10482	48807-48810	NN	denotes	K14
T10484	48810-48811	HYPH	denotes	-
T10483	48811-48816	NN	denotes	Snail
T10486	48817-48819	NN	denotes	Tg
T10485	48820-48824	NNS	denotes	mice
T10472	48825-48831	VBD	denotes	seemed
T10487	48832-48842	JJ	denotes	indicative
T10488	48843-48845	IN	denotes	of
T10489	48846-48847	DT	denotes	a
T10490	48848-48854	NN	denotes	defect
T10491	48855-48857	IN	denotes	in
T10492	48858-48866	NN	denotes	follicle
T10493	48867-48876	NN	denotes	formation
T10494	48877-48878	-LRB-	denotes	(
T10495	48878-48881	VB	denotes	see
T10496	48882-48888	NN	denotes	Figure
T10497	48889-48891	NN	denotes	2A
T10498	48891-48892	-RRB-	denotes	)
T10499	48892-48893	.	denotes	.
T10500	48893-48993	sentence	denotes	Closer inspection, however, revealed that not all hairs penetrated the hyperthickened Tg epidermis.
T10501	48894-48900	JJR	denotes	Closer
T10502	48901-48911	NN	denotes	inspection
T10504	48911-48913	,	denotes	,
T10505	48913-48920	RB	denotes	however
T10506	48920-48922	,	denotes	,
T10503	48922-48930	VBD	denotes	revealed
T10507	48931-48935	IN	denotes	that
T10509	48936-48939	RB	denotes	not
T10511	48940-48943	DT	denotes	all
T10510	48944-48949	NNS	denotes	hairs
T10508	48950-48960	VBD	denotes	penetrated
T10512	48961-48964	DT	denotes	the
T10514	48965-48979	VBN	denotes	hyperthickened
T10515	48980-48982	NN	denotes	Tg
T10513	48983-48992	NN	denotes	epidermis
T10516	48992-48993	.	denotes	.
T10517	48993-49084	sentence	denotes	Several factors may contribute to the seemingly normal follicle development in these mice.
T10518	48994-49001	JJ	denotes	Several
T10519	49002-49009	NNS	denotes	factors
T10521	49010-49013	MD	denotes	may
T10520	49014-49024	VB	denotes	contribute
T10522	49025-49027	IN	denotes	to
T10523	49028-49031	DT	denotes	the
T10525	49032-49041	RB	denotes	seemingly
T10526	49042-49048	JJ	denotes	normal
T10527	49049-49057	NN	denotes	follicle
T10524	49058-49069	NN	denotes	development
T10528	49070-49072	IN	denotes	in
T10529	49073-49078	DT	denotes	these
T10530	49079-49083	NNS	denotes	mice
T10531	49083-49084	.	denotes	.
T10532	49084-49348	sentence	denotes	One obvious factor is the K14 promoter, which is elevated in the basal layer of the epidermis and the outer root sheath (ORS) of the hair follicle, but is markedly down-regulated in developing embryonic hair buds as well as in the postnatal hair progenitor cells.
T10533	49085-49088	CD	denotes	One
T10535	49089-49096	JJ	denotes	obvious
T10534	49097-49103	NN	denotes	factor
T10536	49104-49106	VBZ	denotes	is
T10537	49107-49110	DT	denotes	the
T10539	49111-49114	NN	denotes	K14
T10538	49115-49123	NN	denotes	promoter
T10540	49123-49125	,	denotes	,
T10541	49125-49130	WDT	denotes	which
T10542	49131-49133	VBZ	denotes	is
T10543	49134-49142	JJ	denotes	elevated
T10544	49143-49145	IN	denotes	in
T10545	49146-49149	DT	denotes	the
T10547	49150-49155	JJ	denotes	basal
T10546	49156-49161	NN	denotes	layer
T10548	49162-49164	IN	denotes	of
T10549	49165-49168	DT	denotes	the
T10550	49169-49178	NN	denotes	epidermis
T10551	49179-49182	CC	denotes	and
T10552	49183-49186	DT	denotes	the
T10554	49187-49192	JJ	denotes	outer
T10555	49193-49197	NN	denotes	root
T10553	49198-49204	NN	denotes	sheath
T10556	49205-49206	-LRB-	denotes	(
T10557	49206-49209	NN	denotes	ORS
T10558	49209-49210	-RRB-	denotes	)
T10559	49211-49213	IN	denotes	of
T10560	49214-49217	DT	denotes	the
T10562	49218-49222	NN	denotes	hair
T10561	49223-49231	NN	denotes	follicle
T10563	49231-49233	,	denotes	,
T10564	49233-49236	CC	denotes	but
T10565	49237-49239	VBZ	denotes	is
T10567	49240-49248	RB	denotes	markedly
T10568	49249-49253	RB	denotes	down
T10569	49253-49254	HYPH	denotes	-
T10566	49254-49263	VBN	denotes	regulated
T10570	49264-49266	IN	denotes	in
T10571	49267-49277	JJ	denotes	developing
T10573	49278-49287	JJ	denotes	embryonic
T10574	49288-49292	NN	denotes	hair
T10572	49293-49297	NNS	denotes	buds
T10575	49298-49300	RB	denotes	as
T10577	49301-49305	RB	denotes	well
T10576	49306-49308	IN	denotes	as
T10578	49309-49311	IN	denotes	in
T10579	49312-49315	DT	denotes	the
T10581	49316-49325	JJ	denotes	postnatal
T10582	49326-49330	NN	denotes	hair
T10583	49331-49341	NN	denotes	progenitor
T10580	49342-49347	NNS	denotes	cells
T10584	49347-49348	.	denotes	.
T10585	49348-49508	sentence	denotes	The K14 promoter is also less active in the ORS than epidermis and hence this might also account for the lack of apparent response of the ORS to ectopic Snail.
T10586	49349-49352	DT	denotes	The
T10588	49353-49356	NN	denotes	K14
T10587	49357-49365	NN	denotes	promoter
T10589	49366-49368	VBZ	denotes	is
T10590	49369-49373	RB	denotes	also
T10591	49374-49378	RBR	denotes	less
T10592	49379-49385	JJ	denotes	active
T10593	49386-49388	IN	denotes	in
T10594	49389-49392	DT	denotes	the
T10595	49393-49396	NN	denotes	ORS
T10596	49397-49401	IN	denotes	than
T10597	49402-49411	NN	denotes	epidermis
T10598	49412-49415	CC	denotes	and
T10599	49416-49421	RB	denotes	hence
T10601	49422-49426	DT	denotes	this
T10602	49427-49432	MD	denotes	might
T10603	49433-49437	RB	denotes	also
T10600	49438-49445	VB	denotes	account
T10604	49446-49449	IN	denotes	for
T10605	49450-49453	DT	denotes	the
T10606	49454-49458	NN	denotes	lack
T10607	49459-49461	IN	denotes	of
T10608	49462-49470	JJ	denotes	apparent
T10609	49471-49479	NN	denotes	response
T10610	49480-49482	IN	denotes	of
T10611	49483-49486	DT	denotes	the
T10612	49487-49490	NN	denotes	ORS
T10613	49491-49493	IN	denotes	to
T10614	49494-49501	JJ	denotes	ectopic
T10615	49502-49507	NN	denotes	Snail
T10616	49507-49508	.	denotes	.
T10617	49508-49759	sentence	denotes	Additional contributing factors could be the multiplicity of Snail family members and their differential expression, the saturation, and/or diversity of regulatory mechanisms that govern AJ formation, migration, and proliferation in the follicle ORS.
T10618	49509-49519	JJ	denotes	Additional
T10620	49520-49532	VBG	denotes	contributing
T10619	49533-49540	NNS	denotes	factors
T10622	49541-49546	MD	denotes	could
T10621	49547-49549	VB	denotes	be
T10623	49550-49553	DT	denotes	the
T10624	49554-49566	NN	denotes	multiplicity
T10625	49567-49569	IN	denotes	of
T10626	49570-49575	NN	denotes	Snail
T10628	49576-49582	NN	denotes	family
T10627	49583-49590	NNS	denotes	members
T10629	49591-49594	CC	denotes	and
T10630	49595-49600	PRP$	denotes	their
T10632	49601-49613	JJ	denotes	differential
T10631	49614-49624	NN	denotes	expression
T10633	49624-49626	,	denotes	,
T10634	49626-49629	DT	denotes	the
T10635	49630-49640	NN	denotes	saturation
T10636	49640-49642	,	denotes	,
T10637	49642-49645	CC	denotes	and
T10638	49645-49646	HYPH	denotes	/
T10639	49646-49648	CC	denotes	or
T10640	49649-49658	NN	denotes	diversity
T10641	49659-49661	IN	denotes	of
T10642	49662-49672	JJ	denotes	regulatory
T10643	49673-49683	NNS	denotes	mechanisms
T10644	49684-49688	WDT	denotes	that
T10645	49689-49695	VBP	denotes	govern
T10646	49696-49698	NN	denotes	AJ
T10647	49699-49708	NN	denotes	formation
T10648	49708-49710	,	denotes	,
T10649	49710-49719	NN	denotes	migration
T10650	49719-49721	,	denotes	,
T10651	49721-49724	CC	denotes	and
T10652	49725-49738	NN	denotes	proliferation
T10653	49739-49741	IN	denotes	in
T10654	49742-49745	DT	denotes	the
T10656	49746-49754	NN	denotes	follicle
T10655	49755-49758	NN	denotes	ORS
T10657	49758-49759	.	denotes	.
T10658	49759-49894	sentence	denotes	Distinguishing between these possibilities must await the generation of mice harboring skin-specific loss-of-function Snail mutations.
T10659	49760-49774	VBG	denotes	Distinguishing
T10661	49775-49782	IN	denotes	between
T10662	49783-49788	DT	denotes	these
T10663	49789-49802	NNS	denotes	possibilities
T10664	49803-49807	MD	denotes	must
T10660	49808-49813	VB	denotes	await
T10665	49814-49817	DT	denotes	the
T10666	49818-49828	NN	denotes	generation
T10667	49829-49831	IN	denotes	of
T10668	49832-49836	NNS	denotes	mice
T10669	49837-49846	VBG	denotes	harboring
T10670	49847-49851	NN	denotes	skin
T10672	49851-49852	HYPH	denotes	-
T10671	49852-49860	JJ	denotes	specific
T10674	49861-49865	NN	denotes	loss
T10675	49865-49866	HYPH	denotes	-
T10676	49866-49868	IN	denotes	of
T10677	49868-49869	HYPH	denotes	-
T10678	49869-49877	NN	denotes	function
T10679	49878-49883	NN	denotes	Snail
T10673	49884-49893	NNS	denotes	mutations
T10680	49893-49894	.	denotes	.
T11057	49896-49901	NNS	denotes	Links
T11058	49902-49909	IN	denotes	between
T11059	49910-49919	NN	denotes	Signaling
T11060	49919-49921	,	denotes	,
T11061	49921-49936	JJ	denotes	Transcriptional
T11062	49937-49945	NNS	denotes	Cascades
T11063	49945-49947	,	denotes	,
T11064	49947-49957	JJ	denotes	Epithelial
T11065	49958-49968	NN	denotes	Remodeling
T11066	49968-49970	,	denotes	,
T11067	49970-49973	CC	denotes	and
T11068	49974-49987	NN	denotes	Proliferation
T11069	49988-49990	IN	denotes	in
T11070	49991-49994	DT	denotes	the
T11072	49995-49999	NN	denotes	Hair
T11071	50000-50003	NN	denotes	Bud
T11073	50003-50200	sentence	denotes	Previously, we discovered that early during hair follicle morphogenesis, E-cadherin gene expression is down-regulated concomitantly with the invagination of developing bud cells into the skin [4].
T11074	50004-50014	RB	denotes	Previously
T11076	50014-50016	,	denotes	,
T11077	50016-50018	PRP	denotes	we
T11075	50019-50029	VBD	denotes	discovered
T11078	50030-50034	IN	denotes	that
T11080	50035-50040	RB	denotes	early
T11081	50041-50047	IN	denotes	during
T11082	50048-50052	NN	denotes	hair
T11083	50053-50061	NN	denotes	follicle
T11084	50062-50075	NN	denotes	morphogenesis
T11085	50075-50077	,	denotes	,
T11086	50077-50078	NN	denotes	E
T11088	50078-50079	HYPH	denotes	-
T11087	50079-50087	NN	denotes	cadherin
T11090	50088-50092	NN	denotes	gene
T11089	50093-50103	NN	denotes	expression
T11091	50104-50106	VBZ	denotes	is
T11092	50107-50111	RB	denotes	down
T11093	50111-50112	HYPH	denotes	-
T11079	50112-50121	VBN	denotes	regulated
T11094	50122-50135	RB	denotes	concomitantly
T11095	50136-50140	IN	denotes	with
T11096	50141-50144	DT	denotes	the
T11097	50145-50157	NN	denotes	invagination
T11098	50158-50160	IN	denotes	of
T11099	50161-50171	JJ	denotes	developing
T11101	50172-50175	NN	denotes	bud
T11100	50176-50181	NNS	denotes	cells
T11102	50182-50186	IN	denotes	into
T11103	50187-50190	DT	denotes	the
T11104	50191-50195	NN	denotes	skin
T11105	50196-50197	-LRB-	denotes	[
T11106	50197-50198	CD	denotes	4
T11107	50198-50199	-RRB-	denotes	]
T11108	50199-50200	.	denotes	.
T11109	50200-50419	sentence	denotes	Because the timing of this event correlated with the activation of a LEF-1/β-catenin transcription factor complex [20], we were intrigued by the presence of a putative LEF-1/TCF binding site in the E-cadherin promoter.
T11110	50201-50208	IN	denotes	Because
T11112	50209-50212	DT	denotes	the
T11113	50213-50219	NN	denotes	timing
T11114	50220-50222	IN	denotes	of
T11115	50223-50227	DT	denotes	this
T11116	50228-50233	NN	denotes	event
T11111	50234-50244	VBD	denotes	correlated
T11118	50245-50249	IN	denotes	with
T11119	50250-50253	DT	denotes	the
T11120	50254-50264	NN	denotes	activation
T11121	50265-50267	IN	denotes	of
T11122	50268-50269	DT	denotes	a
T11124	50270-50273	NN	denotes	LEF
T11126	50273-50274	HYPH	denotes	-
T11127	50274-50275	CD	denotes	1
T11128	50275-50276	HYPH	denotes	/
T11129	50276-50277	NN	denotes	β
T11131	50277-50278	HYPH	denotes	-
T11130	50278-50285	NN	denotes	catenin
T11132	50286-50299	NN	denotes	transcription
T11125	50300-50306	NN	denotes	factor
T11123	50307-50314	NN	denotes	complex
T11133	50315-50316	-LRB-	denotes	[
T11134	50316-50318	CD	denotes	20
T11135	50318-50319	-RRB-	denotes	]
T11136	50319-50321	,	denotes	,
T11137	50321-50323	PRP	denotes	we
T11138	50324-50328	VBD	denotes	were
T11117	50329-50338	VBN	denotes	intrigued
T11139	50339-50341	IN	denotes	by
T11140	50342-50345	DT	denotes	the
T11141	50346-50354	NN	denotes	presence
T11142	50355-50357	IN	denotes	of
T11143	50358-50359	DT	denotes	a
T11145	50360-50368	JJ	denotes	putative
T11146	50369-50372	NN	denotes	LEF
T11147	50372-50373	HYPH	denotes	-
T11148	50373-50374	CD	denotes	1
T11149	50374-50375	HYPH	denotes	/
T11150	50375-50378	NN	denotes	TCF
T11151	50379-50386	NN	denotes	binding
T11144	50387-50391	NN	denotes	site
T11152	50392-50394	IN	denotes	in
T11153	50395-50398	DT	denotes	the
T11155	50399-50400	NN	denotes	E
T11157	50400-50401	HYPH	denotes	-
T11156	50401-50409	NN	denotes	cadherin
T11154	50410-50418	NN	denotes	promoter
T11158	50418-50419	.	denotes	.
T11159	50419-50596	sentence	denotes	This prompted an investigation that subsequently led to our discovery that LEF-1/β-catenin can contribute to repression of E-cadherin gene expression in skin keratinocytes [4].
T11160	50420-50424	DT	denotes	This
T11161	50425-50433	VBD	denotes	prompted
T11162	50434-50436	DT	denotes	an
T11163	50437-50450	NN	denotes	investigation
T11164	50451-50455	WDT	denotes	that
T11166	50456-50468	RB	denotes	subsequently
T11165	50469-50472	VBD	denotes	led
T11167	50473-50475	IN	denotes	to
T11168	50476-50479	PRP$	denotes	our
T11169	50480-50489	NN	denotes	discovery
T11170	50490-50494	IN	denotes	that
T11172	50495-50498	NN	denotes	LEF
T11173	50498-50499	HYPH	denotes	-
T11174	50499-50500	CD	denotes	1
T11175	50500-50501	HYPH	denotes	/
T11176	50501-50502	NN	denotes	β
T11178	50502-50503	HYPH	denotes	-
T11177	50503-50510	NN	denotes	catenin
T11179	50511-50514	MD	denotes	can
T11171	50515-50525	VB	denotes	contribute
T11180	50526-50528	IN	denotes	to
T11181	50529-50539	NN	denotes	repression
T11182	50540-50542	IN	denotes	of
T11183	50543-50544	NN	denotes	E
T11185	50544-50545	HYPH	denotes	-
T11184	50545-50553	NN	denotes	cadherin
T11187	50554-50558	NN	denotes	gene
T11186	50559-50569	NN	denotes	expression
T11188	50570-50572	IN	denotes	in
T11189	50573-50577	NN	denotes	skin
T11190	50578-50591	NNS	denotes	keratinocytes
T11191	50592-50593	-LRB-	denotes	[
T11192	50593-50594	CD	denotes	4
T11193	50594-50595	-RRB-	denotes	]
T11194	50595-50596	.	denotes	.
T11195	50596-50848	sentence	denotes	In the course of these studies, we also noted that Snail can also contribute to this process in keratinocytes in vitro, and our present studies revealed that Snail is expressed at the right place and time to be physiologically relevant in the process.
T11196	50597-50599	IN	denotes	In
T11198	50600-50603	DT	denotes	the
T11199	50604-50610	NN	denotes	course
T11200	50611-50613	IN	denotes	of
T11201	50614-50619	DT	denotes	these
T11202	50620-50627	NNS	denotes	studies
T11203	50627-50629	,	denotes	,
T11204	50629-50631	PRP	denotes	we
T11205	50632-50636	RB	denotes	also
T11197	50637-50642	VBD	denotes	noted
T11206	50643-50647	IN	denotes	that
T11208	50648-50653	NN	denotes	Snail
T11209	50654-50657	MD	denotes	can
T11210	50658-50662	RB	denotes	also
T11207	50663-50673	VB	denotes	contribute
T11211	50674-50676	IN	denotes	to
T11212	50677-50681	DT	denotes	this
T11213	50682-50689	NN	denotes	process
T11214	50690-50692	IN	denotes	in
T11215	50693-50706	NNS	denotes	keratinocytes
T11216	50707-50709	FW	denotes	in
T11217	50710-50715	FW	denotes	vitro
T11218	50715-50717	,	denotes	,
T11219	50717-50720	CC	denotes	and
T11220	50721-50724	PRP$	denotes	our
T11222	50725-50732	JJ	denotes	present
T11221	50733-50740	NNS	denotes	studies
T11223	50741-50749	VBD	denotes	revealed
T11224	50750-50754	IN	denotes	that
T11226	50755-50760	NN	denotes	Snail
T11227	50761-50763	VBZ	denotes	is
T11225	50764-50773	VBN	denotes	expressed
T11228	50774-50776	IN	denotes	at
T11229	50777-50780	DT	denotes	the
T11231	50781-50786	JJ	denotes	right
T11230	50787-50792	NN	denotes	place
T11232	50793-50796	CC	denotes	and
T11233	50797-50801	NN	denotes	time
T11234	50802-50804	TO	denotes	to
T11235	50805-50807	VB	denotes	be
T11236	50808-50823	RB	denotes	physiologically
T11237	50824-50832	JJ	denotes	relevant
T11238	50833-50835	IN	denotes	in
T11239	50836-50839	DT	denotes	the
T11240	50840-50847	NN	denotes	process
T11241	50847-50848	.	denotes	.
T11242	50848-51000	sentence	denotes	In noggin-null embryonic skin, LEF-1 expression and subsequent activation of the LEF-1/β-catenin reporter gene is abrogated in the developing placodes.
T11243	50849-50851	IN	denotes	In
T11245	50852-50858	NN	denotes	noggin
T11247	50858-50859	HYPH	denotes	-
T11246	50859-50863	JJ	denotes	null
T11249	50864-50873	JJ	denotes	embryonic
T11248	50874-50878	NN	denotes	skin
T11250	50878-50880	,	denotes	,
T11251	50880-50883	NN	denotes	LEF
T11253	50883-50884	HYPH	denotes	-
T11254	50884-50885	CD	denotes	1
T11252	50886-50896	NN	denotes	expression
T11255	50897-50900	CC	denotes	and
T11256	50901-50911	JJ	denotes	subsequent
T11257	50912-50922	NN	denotes	activation
T11258	50923-50925	IN	denotes	of
T11259	50926-50929	DT	denotes	the
T11261	50930-50933	NN	denotes	LEF
T11262	50933-50934	HYPH	denotes	-
T11263	50934-50935	CD	denotes	1
T11264	50935-50936	HYPH	denotes	/
T11265	50936-50937	NN	denotes	β
T11267	50937-50938	HYPH	denotes	-
T11266	50938-50945	NN	denotes	catenin
T11268	50946-50954	NN	denotes	reporter
T11260	50955-50959	NN	denotes	gene
T11269	50960-50962	VBZ	denotes	is
T11244	50963-50972	VBN	denotes	abrogated
T11270	50973-50975	IN	denotes	in
T11271	50976-50979	DT	denotes	the
T11273	50980-50990	VBG	denotes	developing
T11272	50991-50999	NNS	denotes	placodes
T11274	50999-51000	.	denotes	.
T11275	51000-51163	sentence	denotes	The corresponding failure of E-cadherin down-regulation underscores the importance of Wnt/noggin signaling in regulating this event in follicle morphogenesis [4].
T11276	51001-51004	DT	denotes	The
T11278	51005-51018	VBG	denotes	corresponding
T11277	51019-51026	NN	denotes	failure
T11280	51027-51029	IN	denotes	of
T11281	51030-51031	NN	denotes	E
T11283	51031-51032	HYPH	denotes	-
T11282	51032-51040	NN	denotes	cadherin
T11285	51041-51045	JJ	denotes	down
T11286	51045-51046	HYPH	denotes	-
T11284	51046-51056	NN	denotes	regulation
T11279	51057-51068	VBZ	denotes	underscores
T11287	51069-51072	DT	denotes	the
T11288	51073-51083	NN	denotes	importance
T11289	51084-51086	IN	denotes	of
T11290	51087-51090	NN	denotes	Wnt
T11292	51090-51091	HYPH	denotes	/
T11291	51091-51097	NN	denotes	noggin
T11293	51098-51107	NN	denotes	signaling
T11294	51108-51110	IN	denotes	in
T11295	51111-51121	VBG	denotes	regulating
T11296	51122-51126	DT	denotes	this
T11297	51127-51132	NN	denotes	event
T11298	51133-51135	IN	denotes	in
T11299	51136-51144	NN	denotes	follicle
T11300	51145-51158	NN	denotes	morphogenesis
T11301	51159-51160	-LRB-	denotes	[
T11302	51160-51161	CD	denotes	4
T11303	51161-51162	-RRB-	denotes	]
T11304	51162-51163	.	denotes	.
T11305	51163-51366	sentence	denotes	Conditional gene targeting studies will be necessary to establish whether Snail family members also contribute to the down-regulation in E-cadherin gene expression that occurs during follicle formation.
T11306	51164-51175	JJ	denotes	Conditional
T11308	51176-51180	NN	denotes	gene
T11309	51181-51190	NN	denotes	targeting
T11307	51191-51198	NNS	denotes	studies
T11311	51199-51203	MD	denotes	will
T11310	51204-51206	VB	denotes	be
T11312	51207-51216	JJ	denotes	necessary
T11313	51217-51219	TO	denotes	to
T11314	51220-51229	VB	denotes	establish
T11315	51230-51237	IN	denotes	whether
T11317	51238-51243	NN	denotes	Snail
T11319	51244-51250	NN	denotes	family
T11318	51251-51258	NNS	denotes	members
T11320	51259-51263	RB	denotes	also
T11316	51264-51274	VBP	denotes	contribute
T11321	51275-51277	IN	denotes	to
T11322	51278-51281	DT	denotes	the
T11324	51282-51286	JJ	denotes	down
T11325	51286-51287	HYPH	denotes	-
T11323	51287-51297	NN	denotes	regulation
T11326	51298-51300	IN	denotes	in
T11327	51301-51302	NN	denotes	E
T11329	51302-51303	HYPH	denotes	-
T11328	51303-51311	NN	denotes	cadherin
T11331	51312-51316	NN	denotes	gene
T11330	51317-51327	NN	denotes	expression
T11332	51328-51332	WDT	denotes	that
T11333	51333-51339	VBZ	denotes	occurs
T11334	51340-51346	IN	denotes	during
T11335	51347-51355	NN	denotes	follicle
T11336	51356-51365	NN	denotes	formation
T11337	51365-51366	.	denotes	.
T11338	51366-51535	sentence	denotes	However, it is intriguing that K14-Snail Tg epidermis displayed a marked down-regulation in E-cadherin expression, thereby demonstrating its potential to do so in skin.
T11339	51367-51374	RB	denotes	However
T11341	51374-51376	,	denotes	,
T11342	51376-51378	PRP	denotes	it
T11340	51379-51381	VBZ	denotes	is
T11343	51382-51392	VBG	denotes	intriguing
T11344	51393-51397	IN	denotes	that
T11346	51398-51401	NN	denotes	K14
T11348	51401-51402	HYPH	denotes	-
T11347	51402-51407	NN	denotes	Snail
T11350	51408-51410	NN	denotes	Tg
T11349	51411-51420	NN	denotes	epidermis
T11345	51421-51430	VBD	denotes	displayed
T11351	51431-51432	DT	denotes	a
T11353	51433-51439	JJ	denotes	marked
T11354	51440-51444	JJ	denotes	down
T11355	51444-51445	HYPH	denotes	-
T11352	51445-51455	NN	denotes	regulation
T11356	51456-51458	IN	denotes	in
T11357	51459-51460	NN	denotes	E
T11359	51460-51461	HYPH	denotes	-
T11358	51461-51469	NN	denotes	cadherin
T11360	51470-51480	NN	denotes	expression
T11361	51480-51482	,	denotes	,
T11362	51482-51489	RB	denotes	thereby
T11363	51490-51503	VBG	denotes	demonstrating
T11364	51504-51507	PRP$	denotes	its
T11365	51508-51517	NN	denotes	potential
T11366	51518-51520	TO	denotes	to
T11367	51521-51523	VB	denotes	do
T11368	51524-51526	RB	denotes	so
T11369	51527-51529	IN	denotes	in
T11370	51530-51534	NN	denotes	skin
T11371	51534-51535	.	denotes	.
T11372	51535-51688	sentence	denotes	Our prior findings showed that by elevating E-cadherin levels or by conditionally ablating α-catenin, hair follicle morphogenesis can be impaired [4,7].
T11373	51536-51539	PRP$	denotes	Our
T11375	51540-51545	JJ	denotes	prior
T11374	51546-51554	NNS	denotes	findings
T11376	51555-51561	VBD	denotes	showed
T11377	51562-51566	IN	denotes	that
T11379	51567-51569	IN	denotes	by
T11380	51570-51579	VBG	denotes	elevating
T11381	51580-51581	NN	denotes	E
T11383	51581-51582	HYPH	denotes	-
T11382	51582-51590	NN	denotes	cadherin
T11384	51591-51597	NNS	denotes	levels
T11385	51598-51600	CC	denotes	or
T11386	51601-51603	IN	denotes	by
T11387	51604-51617	RB	denotes	conditionally
T11388	51618-51626	VBG	denotes	ablating
T11389	51627-51628	NN	denotes	α
T11391	51628-51629	HYPH	denotes	-
T11390	51629-51636	NN	denotes	catenin
T11392	51636-51638	,	denotes	,
T11393	51638-51642	NN	denotes	hair
T11394	51643-51651	NN	denotes	follicle
T11395	51652-51665	NN	denotes	morphogenesis
T11396	51666-51669	MD	denotes	can
T11397	51670-51672	VB	denotes	be
T11378	51673-51681	VBN	denotes	impaired
T11398	51682-51683	-LRB-	denotes	[
T11400	51683-51684	CD	denotes	4
T11401	51684-51685	,	denotes	,
T11399	51685-51686	CD	denotes	7
T11402	51686-51687	-RRB-	denotes	]
T11403	51687-51688	.	denotes	.
T11404	51688-52018	sentence	denotes	The marked epidermal hyperproliferation seen in the K14-Snail Tg skin, coupled with the converse suppression of proliferation and Snail in TGF-β2-null hair buds led us to wonder whether the down-regulation of E-cadherin during follicle morphogenesis might have a direct impact on elevating the proliferative state of these cells.
T11405	51689-51692	DT	denotes	The
T11407	51693-51699	JJ	denotes	marked
T11408	51700-51709	JJ	denotes	epidermal
T11406	51710-51728	NN	denotes	hyperproliferation
T11410	51729-51733	VBN	denotes	seen
T11411	51734-51736	IN	denotes	in
T11412	51737-51740	DT	denotes	the
T11414	51741-51744	NN	denotes	K14
T11416	51744-51745	HYPH	denotes	-
T11415	51745-51750	NN	denotes	Snail
T11417	51751-51753	NN	denotes	Tg
T11413	51754-51758	NN	denotes	skin
T11418	51758-51760	,	denotes	,
T11419	51760-51767	VBN	denotes	coupled
T11420	51768-51772	IN	denotes	with
T11421	51773-51776	DT	denotes	the
T11423	51777-51785	JJ	denotes	converse
T11422	51786-51797	NN	denotes	suppression
T11424	51798-51800	IN	denotes	of
T11425	51801-51814	NN	denotes	proliferation
T11426	51815-51818	CC	denotes	and
T11427	51819-51824	NN	denotes	Snail
T11428	51825-51827	IN	denotes	in
T11429	51828-51831	NN	denotes	TGF
T11431	51831-51832	HYPH	denotes	-
T11430	51832-51834	NN	denotes	β2
T11433	51834-51835	HYPH	denotes	-
T11432	51835-51839	JJ	denotes	null
T11435	51840-51844	NN	denotes	hair
T11434	51845-51849	NNS	denotes	buds
T11409	51850-51853	VBD	denotes	led
T11436	51854-51856	PRP	denotes	us
T11437	51857-51859	TO	denotes	to
T11438	51860-51866	VB	denotes	wonder
T11439	51867-51874	IN	denotes	whether
T11441	51875-51878	DT	denotes	the
T11443	51879-51883	JJ	denotes	down
T11444	51883-51884	HYPH	denotes	-
T11442	51884-51894	NN	denotes	regulation
T11445	51895-51897	IN	denotes	of
T11446	51898-51899	NN	denotes	E
T11448	51899-51900	HYPH	denotes	-
T11447	51900-51908	NN	denotes	cadherin
T11449	51909-51915	IN	denotes	during
T11450	51916-51924	NN	denotes	follicle
T11451	51925-51938	NN	denotes	morphogenesis
T11452	51939-51944	MD	denotes	might
T11440	51945-51949	VB	denotes	have
T11453	51950-51951	DT	denotes	a
T11455	51952-51958	JJ	denotes	direct
T11454	51959-51965	NN	denotes	impact
T11456	51966-51968	IN	denotes	on
T11457	51969-51978	VBG	denotes	elevating
T11458	51979-51982	DT	denotes	the
T11460	51983-51996	JJ	denotes	proliferative
T11459	51997-52002	NN	denotes	state
T11461	52003-52005	IN	denotes	of
T11462	52006-52011	DT	denotes	these
T11463	52012-52017	NNS	denotes	cells
T11464	52017-52018	.	denotes	.
T11465	52018-52200	sentence	denotes	Our Tg studies suggested that, at least in part through its regulation of E-cadherin, Snail is able to influence the subcellular localization of a variety of AJ-associated proteins.
T11466	52019-52022	PRP$	denotes	Our
T11468	52023-52025	NN	denotes	Tg
T11467	52026-52033	NNS	denotes	studies
T11469	52034-52043	VBD	denotes	suggested
T11470	52044-52048	IN	denotes	that
T11472	52048-52050	,	denotes	,
T11473	52050-52052	RB	denotes	at
T11474	52053-52058	RBS	denotes	least
T11475	52059-52061	IN	denotes	in
T11477	52062-52066	NN	denotes	part
T11476	52067-52074	IN	denotes	through
T11478	52075-52078	PRP$	denotes	its
T11479	52079-52089	NN	denotes	regulation
T11480	52090-52092	IN	denotes	of
T11481	52093-52094	NN	denotes	E
T11483	52094-52095	HYPH	denotes	-
T11482	52095-52103	NN	denotes	cadherin
T11484	52103-52105	,	denotes	,
T11485	52105-52110	NN	denotes	Snail
T11471	52111-52113	VBZ	denotes	is
T11486	52114-52118	JJ	denotes	able
T11487	52119-52121	TO	denotes	to
T11488	52122-52131	VB	denotes	influence
T11489	52132-52135	DT	denotes	the
T11491	52136-52147	JJ	denotes	subcellular
T11490	52148-52160	NN	denotes	localization
T11492	52161-52163	IN	denotes	of
T11493	52164-52165	DT	denotes	a
T11494	52166-52173	NN	denotes	variety
T11495	52174-52176	IN	denotes	of
T11496	52177-52179	NN	denotes	AJ
T11498	52179-52180	HYPH	denotes	-
T11497	52180-52190	VBN	denotes	associated
T11499	52191-52199	NN	denotes	proteins
T11500	52199-52200	.	denotes	.
T11501	52200-52330	sentence	denotes	One of these appears to be Ajuba, which was previously shown to have the dual capacity to bind Grb-2 as well as α-catenin [9,10].
T11502	52201-52204	CD	denotes	One
T11504	52205-52207	IN	denotes	of
T11505	52208-52213	DT	denotes	these
T11503	52214-52221	VBZ	denotes	appears
T11506	52222-52224	TO	denotes	to
T11507	52225-52227	VB	denotes	be
T11508	52228-52233	NN	denotes	Ajuba
T11509	52233-52235	,	denotes	,
T11510	52235-52240	WDT	denotes	which
T11512	52241-52244	VBD	denotes	was
T11513	52245-52255	RB	denotes	previously
T11511	52256-52261	VBN	denotes	shown
T11514	52262-52264	TO	denotes	to
T11515	52265-52269	VB	denotes	have
T11516	52270-52273	DT	denotes	the
T11518	52274-52278	JJ	denotes	dual
T11517	52279-52287	NN	denotes	capacity
T11519	52288-52290	TO	denotes	to
T11520	52291-52295	VB	denotes	bind
T11521	52296-52299	NN	denotes	Grb
T11522	52299-52300	HYPH	denotes	-
T11523	52300-52301	CD	denotes	2
T11524	52302-52304	RB	denotes	as
T11526	52305-52309	RB	denotes	well
T11525	52310-52312	IN	denotes	as
T11527	52313-52314	NN	denotes	α
T11529	52314-52315	HYPH	denotes	-
T11528	52315-52322	NN	denotes	catenin
T11530	52323-52324	-LRB-	denotes	[
T11532	52324-52325	CD	denotes	9
T11533	52325-52326	,	denotes	,
T11531	52326-52328	CD	denotes	10
T11534	52328-52329	-RRB-	denotes	]
T11535	52329-52330	.	denotes	.
T11536	52330-52558	sentence	denotes	Our studies revealed that in skin keratinocytes that either harbor a conditional null mutation in α-catenin or that overexpress Snail, Ajuba develops an interaction with Grb-2 that is otherwise not observed in WT keratinocytes.
T11537	52331-52334	PRP$	denotes	Our
T11538	52335-52342	NNS	denotes	studies
T11539	52343-52351	VBD	denotes	revealed
T11540	52352-52356	IN	denotes	that
T11542	52357-52359	IN	denotes	in
T11543	52360-52364	NN	denotes	skin
T11544	52365-52378	NNS	denotes	keratinocytes
T11545	52379-52383	WDT	denotes	that
T11547	52384-52390	CC	denotes	either
T11546	52391-52397	VBP	denotes	harbor
T11548	52398-52399	DT	denotes	a
T11550	52400-52411	JJ	denotes	conditional
T11551	52412-52416	JJ	denotes	null
T11549	52417-52425	NN	denotes	mutation
T11552	52426-52428	IN	denotes	in
T11553	52429-52430	NN	denotes	α
T11555	52430-52431	HYPH	denotes	-
T11554	52431-52438	NN	denotes	catenin
T11556	52439-52441	CC	denotes	or
T11557	52442-52446	WDT	denotes	that
T11558	52447-52458	VBP	denotes	overexpress
T11559	52459-52464	NN	denotes	Snail
T11560	52464-52466	,	denotes	,
T11561	52466-52471	NN	denotes	Ajuba
T11541	52472-52480	VBZ	denotes	develops
T11562	52481-52483	DT	denotes	an
T11563	52484-52495	NN	denotes	interaction
T11564	52496-52500	IN	denotes	with
T11565	52501-52504	NN	denotes	Grb
T11566	52504-52505	HYPH	denotes	-
T11567	52505-52506	CD	denotes	2
T11568	52507-52511	WDT	denotes	that
T11570	52512-52514	VBZ	denotes	is
T11571	52515-52524	RB	denotes	otherwise
T11572	52525-52528	RB	denotes	not
T11569	52529-52537	VBN	denotes	observed
T11573	52538-52540	IN	denotes	in
T11574	52541-52543	NN	denotes	WT
T11575	52544-52557	NNS	denotes	keratinocytes
T11576	52557-52558	.	denotes	.
T11577	52558-52862	sentence	denotes	The corresponding abilities of either Snail-transfected or Ajuba-transfected keratinocytes to exhibit elevated activation of the Ras-MAPK pathway suggest that the Grb-2 association of Ajuba under conditions of reduced levels of AJ proteins may be directly relevant to the parallel in hyperproliferation.
T11578	52559-52562	DT	denotes	The
T11580	52563-52576	VBG	denotes	corresponding
T11579	52577-52586	NNS	denotes	abilities
T11582	52587-52589	IN	denotes	of
T11583	52590-52596	CC	denotes	either
T11585	52597-52602	NN	denotes	Snail
T11587	52602-52603	HYPH	denotes	-
T11586	52603-52614	VBN	denotes	transfected
T11588	52615-52617	CC	denotes	or
T11589	52618-52623	NN	denotes	Ajuba
T11591	52623-52624	HYPH	denotes	-
T11590	52624-52635	VBN	denotes	transfected
T11584	52636-52649	NNS	denotes	keratinocytes
T11592	52650-52652	TO	denotes	to
T11593	52653-52660	VB	denotes	exhibit
T11594	52661-52669	JJ	denotes	elevated
T11595	52670-52680	NN	denotes	activation
T11596	52681-52683	IN	denotes	of
T11597	52684-52687	DT	denotes	the
T11599	52688-52691	NN	denotes	Ras
T11601	52691-52692	HYPH	denotes	-
T11600	52692-52696	NN	denotes	MAPK
T11598	52697-52704	NN	denotes	pathway
T11581	52705-52712	VBP	denotes	suggest
T11602	52713-52717	IN	denotes	that
T11604	52718-52721	DT	denotes	the
T11606	52722-52725	NN	denotes	Grb
T11607	52725-52726	HYPH	denotes	-
T11608	52726-52727	CD	denotes	2
T11605	52728-52739	NN	denotes	association
T11609	52740-52742	IN	denotes	of
T11610	52743-52748	NN	denotes	Ajuba
T11611	52749-52754	IN	denotes	under
T11612	52755-52765	NNS	denotes	conditions
T11613	52766-52768	IN	denotes	of
T11614	52769-52776	JJ	denotes	reduced
T11615	52777-52783	NNS	denotes	levels
T11616	52784-52786	IN	denotes	of
T11617	52787-52789	NN	denotes	AJ
T11618	52790-52798	NN	denotes	proteins
T11619	52799-52802	MD	denotes	may
T11603	52803-52805	VB	denotes	be
T11620	52806-52814	RB	denotes	directly
T11621	52815-52823	JJ	denotes	relevant
T11622	52824-52826	IN	denotes	to
T11623	52827-52830	DT	denotes	the
T11624	52831-52839	NN	denotes	parallel
T11625	52840-52842	IN	denotes	in
T11626	52843-52861	NN	denotes	hyperproliferation
T11627	52861-52862	.	denotes	.
T11628	52862-53049	sentence	denotes	In stable epithelial (i.e., Madin-Darby canine kidney, or MDCK) cell lines, Snail has been shown to block cell cycle progression and promote motility and shape changes for invasion [47].
T11629	52863-52865	IN	denotes	In
T11631	52866-52872	JJ	denotes	stable
T11633	52873-52883	JJ	denotes	epithelial
T11634	52884-52885	-LRB-	denotes	(
T11636	52885-52889	FW	denotes	i.e.
T11637	52889-52891	,	denotes	,
T11638	52891-52896	NNP	denotes	Madin
T11640	52896-52897	HYPH	denotes	-
T11639	52897-52902	NNP	denotes	Darby
T11641	52903-52909	JJ	denotes	canine
T11635	52910-52916	NN	denotes	kidney
T11642	52916-52918	,	denotes	,
T11643	52918-52920	CC	denotes	or
T11644	52921-52925	NN	denotes	MDCK
T11645	52925-52926	-RRB-	denotes	)
T11646	52927-52931	NN	denotes	cell
T11632	52932-52937	NNS	denotes	lines
T11647	52937-52939	,	denotes	,
T11648	52939-52944	NN	denotes	Snail
T11649	52945-52948	VBZ	denotes	has
T11650	52949-52953	VBN	denotes	been
T11630	52954-52959	VBN	denotes	shown
T11651	52960-52962	TO	denotes	to
T11652	52963-52968	VB	denotes	block
T11653	52969-52973	NN	denotes	cell
T11654	52974-52979	NN	denotes	cycle
T11655	52980-52991	NN	denotes	progression
T11656	52992-52995	CC	denotes	and
T11657	52996-53003	VB	denotes	promote
T11658	53004-53012	NN	denotes	motility
T11660	53013-53016	CC	denotes	and
T11661	53017-53022	NN	denotes	shape
T11659	53023-53030	NNS	denotes	changes
T11662	53031-53034	IN	denotes	for
T11663	53035-53043	NN	denotes	invasion
T11664	53044-53045	-LRB-	denotes	[
T11665	53045-53047	CD	denotes	47
T11666	53047-53048	-RRB-	denotes	]
T11667	53048-53049	.	denotes	.
T11668	53049-53219	sentence	denotes	While our in vivo studies are consistent with a role for Snail in motility and epithelial remodeling, they differ with respect to Snail's apparent proliferative effects.
T11669	53050-53055	IN	denotes	While
T11671	53056-53059	PRP$	denotes	our
T11673	53060-53062	FW	denotes	in
T11674	53063-53067	FW	denotes	vivo
T11672	53068-53075	NNS	denotes	studies
T11670	53076-53079	VBP	denotes	are
T11676	53080-53090	JJ	denotes	consistent
T11677	53091-53095	IN	denotes	with
T11678	53096-53097	DT	denotes	a
T11679	53098-53102	NN	denotes	role
T11680	53103-53106	IN	denotes	for
T11681	53107-53112	NN	denotes	Snail
T11682	53113-53115	IN	denotes	in
T11683	53116-53124	NN	denotes	motility
T11685	53125-53128	CC	denotes	and
T11686	53129-53139	JJ	denotes	epithelial
T11684	53140-53150	NN	denotes	remodeling
T11687	53150-53152	,	denotes	,
T11688	53152-53156	PRP	denotes	they
T11675	53157-53163	VBP	denotes	differ
T11689	53164-53168	IN	denotes	with
T11690	53169-53176	NN	denotes	respect
T11691	53177-53179	IN	denotes	to
T11692	53180-53185	NN	denotes	Snail
T11694	53185-53187	POS	denotes	's
T11695	53188-53196	JJ	denotes	apparent
T11696	53197-53210	JJ	denotes	proliferative
T11693	53211-53218	NNS	denotes	effects
T11697	53218-53219	.	denotes	.
T11698	53219-53329	sentence	denotes	A priori, this could be simply due to variations in the response of different cell types to Snail expression.
T11699	53220-53221	FW	denotes	A
T11700	53222-53228	FW	denotes	priori
T11702	53228-53230	,	denotes	,
T11703	53230-53234	DT	denotes	this
T11704	53235-53240	MD	denotes	could
T11701	53241-53243	VB	denotes	be
T11705	53244-53250	RB	denotes	simply
T11706	53251-53254	IN	denotes	due
T11707	53255-53257	IN	denotes	to
T11708	53258-53268	NNS	denotes	variations
T11709	53269-53271	IN	denotes	in
T11710	53272-53275	DT	denotes	the
T11711	53276-53284	NN	denotes	response
T11712	53285-53287	IN	denotes	of
T11713	53288-53297	JJ	denotes	different
T11715	53298-53302	NN	denotes	cell
T11714	53303-53308	NNS	denotes	types
T11716	53309-53311	IN	denotes	to
T11717	53312-53317	NN	denotes	Snail
T11718	53318-53328	NN	denotes	expression
T11719	53328-53329	.	denotes	.
T11720	53329-53488	sentence	denotes	Alternatively, these differences may be relevant to the benefit of using mouse models to reveal functions not always recapitulated in stable cell line models.
T11721	53330-53343	RB	denotes	Alternatively
T11723	53343-53345	,	denotes	,
T11724	53345-53350	DT	denotes	these
T11725	53351-53362	NNS	denotes	differences
T11726	53363-53366	MD	denotes	may
T11722	53367-53369	VB	denotes	be
T11727	53370-53378	JJ	denotes	relevant
T11728	53379-53381	IN	denotes	to
T11729	53382-53385	DT	denotes	the
T11730	53386-53393	NN	denotes	benefit
T11731	53394-53396	IN	denotes	of
T11732	53397-53402	VBG	denotes	using
T11733	53403-53408	NN	denotes	mouse
T11734	53409-53415	NNS	denotes	models
T11735	53416-53418	TO	denotes	to
T11736	53419-53425	VB	denotes	reveal
T11737	53426-53435	NNS	denotes	functions
T11738	53436-53439	RB	denotes	not
T11740	53440-53446	RB	denotes	always
T11739	53447-53460	VBN	denotes	recapitulated
T11741	53461-53463	IN	denotes	in
T11742	53464-53470	JJ	denotes	stable
T11744	53471-53475	NN	denotes	cell
T11745	53476-53480	NN	denotes	line
T11743	53481-53487	NNS	denotes	models
T11746	53487-53488	.	denotes	.
T11747	53488-53571	sentence	denotes	Future studies should highlight the underlying reasons for these opposing results.
T11748	53489-53495	JJ	denotes	Future
T11749	53496-53503	NNS	denotes	studies
T11751	53504-53510	MD	denotes	should
T11750	53511-53520	VB	denotes	highlight
T11752	53521-53524	DT	denotes	the
T11754	53525-53535	JJ	denotes	underlying
T11753	53536-53543	NNS	denotes	reasons
T11755	53544-53547	IN	denotes	for
T11756	53548-53553	DT	denotes	these
T11758	53554-53562	VBG	denotes	opposing
T11757	53563-53570	NNS	denotes	results
T11759	53570-53571	.	denotes	.
T11760	53571-53756	sentence	denotes	Irrespective of these differences, our in vivo studies do not stand alone, as there are many situations in which a down-regulation in AJ proteins correlate with enhanced proliferation.
T11761	53572-53584	JJ	denotes	Irrespective
T11763	53585-53587	IN	denotes	of
T11764	53588-53593	DT	denotes	these
T11765	53594-53605	NNS	denotes	differences
T11766	53605-53607	,	denotes	,
T11767	53607-53610	PRP$	denotes	our
T11769	53611-53613	FW	denotes	in
T11770	53614-53618	FW	denotes	vivo
T11768	53619-53626	NNS	denotes	studies
T11771	53627-53629	VBP	denotes	do
T11772	53630-53633	RB	denotes	not
T11762	53634-53639	VB	denotes	stand
T11773	53640-53645	RB	denotes	alone
T11774	53645-53647	,	denotes	,
T11775	53647-53649	IN	denotes	as
T11777	53650-53655	EX	denotes	there
T11776	53656-53659	VBP	denotes	are
T11778	53660-53664	JJ	denotes	many
T11779	53665-53675	NNS	denotes	situations
T11780	53676-53678	IN	denotes	in
T11782	53679-53684	WDT	denotes	which
T11783	53685-53686	DT	denotes	a
T11785	53687-53691	JJ	denotes	down
T11786	53691-53692	HYPH	denotes	-
T11784	53692-53702	NN	denotes	regulation
T11787	53703-53705	IN	denotes	in
T11788	53706-53708	NN	denotes	AJ
T11789	53709-53717	NN	denotes	proteins
T11781	53718-53727	VBP	denotes	correlate
T11790	53728-53732	IN	denotes	with
T11791	53733-53741	VBN	denotes	enhanced
T11792	53742-53755	NN	denotes	proliferation
T11793	53755-53756	.	denotes	.
T11794	53756-53906	sentence	denotes	In fact, a myriad of diverse mechanisms have been implicated in activating epithelial proliferation upon down-regulation of AJ proteins [7,23,24,48].
T11795	53757-53759	IN	denotes	In
T11797	53760-53764	NN	denotes	fact
T11798	53764-53766	,	denotes	,
T11799	53766-53767	DT	denotes	a
T11800	53768-53774	NN	denotes	myriad
T11801	53775-53777	IN	denotes	of
T11802	53778-53785	JJ	denotes	diverse
T11803	53786-53796	NNS	denotes	mechanisms
T11804	53797-53801	VBP	denotes	have
T11805	53802-53806	VBN	denotes	been
T11796	53807-53817	VBN	denotes	implicated
T11806	53818-53820	IN	denotes	in
T11807	53821-53831	VBG	denotes	activating
T11808	53832-53842	JJ	denotes	epithelial
T11809	53843-53856	NN	denotes	proliferation
T11810	53857-53861	IN	denotes	upon
T11811	53862-53866	JJ	denotes	down
T11813	53866-53867	HYPH	denotes	-
T11812	53867-53877	NN	denotes	regulation
T11814	53878-53880	IN	denotes	of
T11815	53881-53883	NN	denotes	AJ
T11816	53884-53892	NN	denotes	proteins
T11817	53893-53894	-LRB-	denotes	[
T11819	53894-53895	CD	denotes	7
T11820	53895-53896	,	denotes	,
T11821	53896-53898	CD	denotes	23
T11822	53898-53899	,	denotes	,
T11823	53899-53901	CD	denotes	24
T11824	53901-53902	,	denotes	,
T11818	53902-53904	CD	denotes	48
T11825	53904-53905	-RRB-	denotes	]
T11826	53905-53906	.	denotes	.
T11827	53906-54001	sentence	denotes	Sifting through these converging pathways is likely to be a difficult and painstaking process.
T11828	53907-53914	VBG	denotes	Sifting
T11830	53915-53922	IN	denotes	through
T11831	53923-53928	DT	denotes	these
T11833	53929-53939	VBG	denotes	converging
T11832	53940-53948	NNS	denotes	pathways
T11829	53949-53951	VBZ	denotes	is
T11834	53952-53958	JJ	denotes	likely
T11835	53959-53961	TO	denotes	to
T11836	53962-53964	VB	denotes	be
T11837	53965-53966	DT	denotes	a
T11839	53967-53976	JJ	denotes	difficult
T11840	53977-53980	CC	denotes	and
T11841	53981-53992	JJ	denotes	painstaking
T11838	53993-54000	NN	denotes	process
T11842	54000-54001	.	denotes	.
T11843	54001-54286	sentence	denotes	This said, by identifying the status of different players involved in specific cell types and at specific stages in development, our mechanistic understanding of how intercellular remodeling is linked to proliferation in epithelial morphogenesis should begin to surface in the future.
T11844	54002-54006	DT	denotes	This
T11845	54007-54011	VBD	denotes	said
T11847	54011-54013	,	denotes	,
T11848	54013-54015	IN	denotes	by
T11849	54016-54027	VBG	denotes	identifying
T11850	54028-54031	DT	denotes	the
T11851	54032-54038	NN	denotes	status
T11852	54039-54041	IN	denotes	of
T11853	54042-54051	JJ	denotes	different
T11854	54052-54059	NNS	denotes	players
T11855	54060-54068	VBN	denotes	involved
T11856	54069-54071	IN	denotes	in
T11857	54072-54080	JJ	denotes	specific
T11859	54081-54085	NN	denotes	cell
T11858	54086-54091	NNS	denotes	types
T11860	54092-54095	CC	denotes	and
T11861	54096-54098	IN	denotes	at
T11862	54099-54107	JJ	denotes	specific
T11863	54108-54114	NNS	denotes	stages
T11864	54115-54117	IN	denotes	in
T11865	54118-54129	NN	denotes	development
T11866	54129-54131	,	denotes	,
T11867	54131-54134	PRP$	denotes	our
T11869	54135-54146	JJ	denotes	mechanistic
T11868	54147-54160	NN	denotes	understanding
T11870	54161-54163	IN	denotes	of
T11871	54164-54167	WRB	denotes	how
T11873	54168-54181	JJ	denotes	intercellular
T11874	54182-54192	NN	denotes	remodeling
T11875	54193-54195	VBZ	denotes	is
T11872	54196-54202	VBN	denotes	linked
T11876	54203-54205	IN	denotes	to
T11877	54206-54219	NN	denotes	proliferation
T11878	54220-54222	IN	denotes	in
T11879	54223-54233	JJ	denotes	epithelial
T11880	54234-54247	NN	denotes	morphogenesis
T11881	54248-54254	MD	denotes	should
T11846	54255-54260	VB	denotes	begin
T11882	54261-54263	TO	denotes	to
T11883	54264-54271	VB	denotes	surface
T11884	54272-54274	IN	denotes	in
T11885	54275-54278	DT	denotes	the
T11886	54279-54285	NN	denotes	future
T11887	54285-54286	.	denotes	.
T11888	54286-54452	sentence	denotes	Elucidating the molecular mechanisms through which these networks converge is also a prerequisite for understanding how these processes go awry during tumorigenesis.
T11889	54287-54298	VBG	denotes	Elucidating
T11891	54299-54302	DT	denotes	the
T11893	54303-54312	JJ	denotes	molecular
T11892	54313-54323	NNS	denotes	mechanisms
T11894	54324-54331	IN	denotes	through
T11896	54332-54337	WDT	denotes	which
T11897	54338-54343	DT	denotes	these
T11898	54344-54352	NNS	denotes	networks
T11895	54353-54361	VBP	denotes	converge
T11890	54362-54364	VBZ	denotes	is
T11899	54365-54369	RB	denotes	also
T11900	54370-54371	DT	denotes	a
T11901	54372-54384	NN	denotes	prerequisite
T11902	54385-54388	IN	denotes	for
T11903	54389-54402	VBG	denotes	understanding
T11904	54403-54406	WRB	denotes	how
T11906	54407-54412	DT	denotes	these
T11907	54413-54422	NNS	denotes	processes
T11905	54423-54425	VBP	denotes	go
T11908	54426-54430	RB	denotes	awry
T11909	54431-54437	IN	denotes	during
T11910	54438-54451	NN	denotes	tumorigenesis
T11911	54451-54452	.	denotes	.
T12021	54477-54485	NNS	denotes	Reagents
T12022	54485-55576	sentence	denotes	Primary antibodies used were against: E-cadherin (M. Takeichi, Kyoto University, Japan); α-catenin, β-catenin, pMAPK, tubulin (Sigma, St. Louis, Missouri, United States), Ajuba (G. Longmore, Washington University, St. Louis, Missouri, United States); β4 integrin/CD104 (BD Pharmingen, San Diego, California, United States), laminin 5 (R. Burgeson, Harvard University, Cambridge, Massachusetts, United States), K5, K1, loricrin (Fuchs Lab), involucrin, fillagrin (Covance, Berkeley, California, United States), MAPK, pSMAD2 (Cell Signaling, Beverly, Massachusetts, United States); Grb-2 (Santa Cruz Biotech, Santa Cruz, California, United States); P-cadherin (Zymed Laboratories, South San Francisco, California, United States); HA (Roche Biochemicals), vimentin (Chemicon, Temecula, California, United States), Ki67 (Novo Castra, Newcastle Upon Tyne, United Kingdom), keratin 6 (P. Coulombe, John Hopkins University, Baltimore, Maryland, United States), cyclin D (Oncogene, San Diego, California, United States), and TGF-β2 (L. Gold, New York University, New York, New York, United States).
T12023	54486-54493	JJ	denotes	Primary
T12024	54494-54504	NNS	denotes	antibodies
T12026	54505-54509	VBN	denotes	used
T12025	54510-54514	VBD	denotes	were
T12027	54515-54522	IN	denotes	against
T12028	54522-54524	:	denotes	:
T12029	54524-54525	NN	denotes	E
T12031	54525-54526	HYPH	denotes	-
T12030	54526-54534	NN	denotes	cadherin
T12032	54535-54536	-LRB-	denotes	(
T12034	54536-54538	NNP	denotes	M.
T12033	54539-54547	NNP	denotes	Takeichi
T12035	54547-54549	,	denotes	,
T12036	54549-54554	NNP	denotes	Kyoto
T12037	54555-54565	NNP	denotes	University
T12038	54565-54567	,	denotes	,
T12039	54567-54572	NNP	denotes	Japan
T12040	54572-54573	-RRB-	denotes	)
T12041	54573-54574	:	denotes	;
T12042	54575-54576	NN	denotes	α
T12044	54576-54577	HYPH	denotes	-
T12043	54577-54584	NN	denotes	catenin
T12045	54584-54586	,	denotes	,
T12046	54586-54587	NN	denotes	β
T12048	54587-54588	HYPH	denotes	-
T12047	54588-54595	NN	denotes	catenin
T12049	54595-54597	,	denotes	,
T12050	54597-54602	NN	denotes	pMAPK
T12051	54602-54604	,	denotes	,
T12052	54604-54611	NN	denotes	tubulin
T12053	54612-54613	-LRB-	denotes	(
T12054	54613-54618	NNP	denotes	Sigma
T12055	54618-54620	,	denotes	,
T12056	54620-54623	NNP	denotes	St.
T12057	54624-54629	NNP	denotes	Louis
T12058	54629-54631	,	denotes	,
T12059	54631-54639	NNP	denotes	Missouri
T12060	54639-54641	,	denotes	,
T12061	54641-54647	NNP	denotes	United
T12062	54648-54654	NNP	denotes	States
T12063	54654-54655	-RRB-	denotes	)
T12064	54655-54657	,	denotes	,
T12065	54657-54662	NN	denotes	Ajuba
T12066	54663-54664	-LRB-	denotes	(
T12068	54664-54666	NNP	denotes	G.
T12067	54667-54675	NNP	denotes	Longmore
T12069	54675-54677	,	denotes	,
T12070	54677-54687	NNP	denotes	Washington
T12071	54688-54698	NNP	denotes	University
T12072	54698-54700	,	denotes	,
T12073	54700-54703	NNP	denotes	St.
T12074	54704-54709	NNP	denotes	Louis
T12075	54709-54711	,	denotes	,
T12076	54711-54719	NNP	denotes	Missouri
T12077	54719-54721	,	denotes	,
T12078	54721-54727	NNP	denotes	United
T12079	54728-54734	NNP	denotes	States
T12080	54734-54735	-RRB-	denotes	)
T12081	54735-54736	:	denotes	;
T12082	54737-54739	NN	denotes	β4
T12083	54740-54748	NN	denotes	integrin
T12084	54748-54749	HYPH	denotes	/
T12085	54749-54754	NN	denotes	CD104
T12086	54755-54756	-LRB-	denotes	(
T12088	54756-54758	NNP	denotes	BD
T12087	54759-54769	NNP	denotes	Pharmingen
T12089	54769-54771	,	denotes	,
T12090	54771-54774	NNP	denotes	San
T12091	54775-54780	NNP	denotes	Diego
T12092	54780-54782	,	denotes	,
T12093	54782-54792	NNP	denotes	California
T12094	54792-54794	,	denotes	,
T12095	54794-54800	NNP	denotes	United
T12096	54801-54807	NNP	denotes	States
T12097	54807-54808	-RRB-	denotes	)
T12098	54808-54810	,	denotes	,
T12099	54810-54817	NN	denotes	laminin
T12100	54818-54819	CD	denotes	5
T12101	54820-54821	-LRB-	denotes	(
T12103	54821-54823	NNP	denotes	R.
T12102	54824-54832	NNP	denotes	Burgeson
T12104	54832-54834	,	denotes	,
T12105	54834-54841	NNP	denotes	Harvard
T12106	54842-54852	NNP	denotes	University
T12107	54852-54854	,	denotes	,
T12108	54854-54863	NNP	denotes	Cambridge
T12109	54863-54865	,	denotes	,
T12110	54865-54878	NNP	denotes	Massachusetts
T12111	54878-54880	,	denotes	,
T12112	54880-54886	NNP	denotes	United
T12113	54887-54893	NNP	denotes	States
T12114	54893-54894	-RRB-	denotes	)
T12115	54894-54896	,	denotes	,
T12116	54896-54898	NN	denotes	K5
T12117	54898-54900	,	denotes	,
T12118	54900-54902	NN	denotes	K1
T12119	54902-54904	,	denotes	,
T12120	54904-54912	NN	denotes	loricrin
T12121	54913-54914	-LRB-	denotes	(
T12123	54914-54919	NNP	denotes	Fuchs
T12122	54920-54923	NNP	denotes	Lab
T12124	54923-54924	-RRB-	denotes	)
T12125	54924-54926	,	denotes	,
T12126	54926-54936	NN	denotes	involucrin
T12127	54936-54938	,	denotes	,
T12128	54938-54947	NN	denotes	fillagrin
T12129	54948-54949	-LRB-	denotes	(
T12130	54949-54956	NNP	denotes	Covance
T12131	54956-54958	,	denotes	,
T12132	54958-54966	NNP	denotes	Berkeley
T12133	54966-54968	,	denotes	,
T12134	54968-54978	NNP	denotes	California
T12135	54978-54980	,	denotes	,
T12136	54980-54986	NNP	denotes	United
T12137	54987-54993	NNP	denotes	States
T12138	54993-54994	-RRB-	denotes	)
T12139	54994-54996	,	denotes	,
T12140	54996-55000	NN	denotes	MAPK
T12141	55000-55002	,	denotes	,
T12142	55002-55008	NN	denotes	pSMAD2
T12143	55009-55010	-LRB-	denotes	(
T12145	55010-55014	NNP	denotes	Cell
T12144	55015-55024	NNP	denotes	Signaling
T12146	55024-55026	,	denotes	,
T12147	55026-55033	NNP	denotes	Beverly
T12148	55033-55035	,	denotes	,
T12149	55035-55048	NNP	denotes	Massachusetts
T12150	55048-55050	,	denotes	,
T12151	55050-55056	NNP	denotes	United
T12152	55057-55063	NNP	denotes	States
T12153	55063-55064	-RRB-	denotes	)
T12154	55064-55065	:	denotes	;
T12155	55066-55069	NN	denotes	Grb
T12156	55069-55070	HYPH	denotes	-
T12157	55070-55071	CD	denotes	2
T12158	55072-55073	-LRB-	denotes	(
T12160	55073-55078	NNP	denotes	Santa
T12161	55079-55083	NNP	denotes	Cruz
T12159	55084-55091	NNP	denotes	Biotech
T12162	55091-55093	,	denotes	,
T12163	55093-55098	NNP	denotes	Santa
T12164	55099-55103	NNP	denotes	Cruz
T12165	55103-55105	,	denotes	,
T12166	55105-55115	NNP	denotes	California
T12167	55115-55117	,	denotes	,
T12168	55117-55123	NNP	denotes	United
T12169	55124-55130	NNP	denotes	States
T12170	55130-55131	-RRB-	denotes	)
T12171	55131-55132	:	denotes	;
T12172	55133-55134	NN	denotes	P
T12174	55134-55135	HYPH	denotes	-
T12173	55135-55143	NN	denotes	cadherin
T12175	55144-55145	-LRB-	denotes	(
T12177	55145-55150	NNP	denotes	Zymed
T12176	55151-55163	NNP	denotes	Laboratories
T12178	55163-55165	,	denotes	,
T12179	55165-55170	JJ	denotes	South
T12181	55171-55174	NNP	denotes	San
T12180	55175-55184	NNP	denotes	Francisco
T12182	55184-55186	,	denotes	,
T12183	55186-55196	NNP	denotes	California
T12184	55196-55198	,	denotes	,
T12185	55198-55204	NNP	denotes	United
T12186	55205-55211	NNP	denotes	States
T12187	55211-55212	-RRB-	denotes	)
T12188	55212-55213	:	denotes	;
T12189	55214-55216	NN	denotes	HA
T12190	55217-55218	-LRB-	denotes	(
T12192	55218-55223	NNP	denotes	Roche
T12191	55224-55236	NNP	denotes	Biochemicals
T12193	55236-55237	-RRB-	denotes	)
T12194	55237-55239	,	denotes	,
T12195	55239-55247	NN	denotes	vimentin
T12196	55248-55249	-LRB-	denotes	(
T12197	55249-55257	NNP	denotes	Chemicon
T12198	55257-55259	,	denotes	,
T12199	55259-55267	NNP	denotes	Temecula
T12200	55267-55269	,	denotes	,
T12201	55269-55279	NNP	denotes	California
T12202	55279-55281	,	denotes	,
T12203	55281-55287	NNP	denotes	United
T12204	55288-55294	NNP	denotes	States
T12205	55294-55295	-RRB-	denotes	)
T12206	55295-55297	,	denotes	,
T12207	55297-55301	NN	denotes	Ki67
T12208	55302-55303	-LRB-	denotes	(
T12210	55303-55307	NNP	denotes	Novo
T12209	55308-55314	NNP	denotes	Castra
T12211	55314-55316	,	denotes	,
T12212	55316-55325	NNP	denotes	Newcastle
T12213	55326-55330	IN	denotes	Upon
T12214	55331-55335	NNP	denotes	Tyne
T12215	55335-55337	,	denotes	,
T12216	55337-55343	NNP	denotes	United
T12217	55344-55351	NNP	denotes	Kingdom
T12218	55351-55352	-RRB-	denotes	)
T12219	55352-55354	,	denotes	,
T12220	55354-55361	NN	denotes	keratin
T12221	55362-55363	CD	denotes	6
T12222	55364-55365	-LRB-	denotes	(
T12224	55365-55367	NNP	denotes	P.
T12223	55368-55376	NNP	denotes	Coulombe
T12225	55376-55378	,	denotes	,
T12226	55378-55382	NNP	denotes	John
T12227	55383-55390	NNP	denotes	Hopkins
T12228	55391-55401	NNP	denotes	University
T12229	55401-55403	,	denotes	,
T12230	55403-55412	NNP	denotes	Baltimore
T12231	55412-55414	,	denotes	,
T12232	55414-55422	NNP	denotes	Maryland
T12233	55422-55424	,	denotes	,
T12234	55424-55430	NNP	denotes	United
T12235	55431-55437	NNP	denotes	States
T12236	55437-55438	-RRB-	denotes	)
T12237	55438-55440	,	denotes	,
T12238	55440-55446	NN	denotes	cyclin
T12239	55447-55448	NN	denotes	D
T12240	55449-55450	-LRB-	denotes	(
T12241	55450-55458	NNP	denotes	Oncogene
T12242	55458-55460	,	denotes	,
T12243	55460-55463	NNP	denotes	San
T12244	55464-55469	NNP	denotes	Diego
T12245	55469-55471	,	denotes	,
T12246	55471-55481	NNP	denotes	California
T12247	55481-55483	,	denotes	,
T12248	55483-55489	NNP	denotes	United
T12249	55490-55496	NNP	denotes	States
T12250	55496-55497	-RRB-	denotes	)
T12251	55497-55499	,	denotes	,
T12252	55499-55502	CC	denotes	and
T12253	55503-55506	NN	denotes	TGF
T12255	55506-55507	HYPH	denotes	-
T12254	55507-55509	NN	denotes	β2
T12256	55510-55511	-LRB-	denotes	(
T12258	55511-55513	NNP	denotes	L.
T12257	55514-55518	NNP	denotes	Gold
T12259	55518-55520	,	denotes	,
T12260	55520-55523	NNP	denotes	New
T12261	55524-55528	NNP	denotes	York
T12262	55529-55539	NNP	denotes	University
T12263	55539-55541	,	denotes	,
T12264	55541-55544	NNP	denotes	New
T12265	55545-55549	NNP	denotes	York
T12266	55549-55551	,	denotes	,
T12267	55551-55554	NNP	denotes	New
T12268	55555-55559	NNP	denotes	York
T12269	55559-55561	,	denotes	,
T12270	55561-55567	NNP	denotes	United
T12271	55568-55574	NNP	denotes	States
T12272	55574-55575	-RRB-	denotes	)
T12273	55575-55576	.	denotes	.
T12274	55576-55710	sentence	denotes	FITC-, Texas Red-, or HRP-conjugated secondary antibodies were from Jackson ImmunoResearch (West Grove, Pennsylvania, United States).
T12275	55577-55581	NN	denotes	FITC
T12277	55581-55582	HYPH	denotes	-
T12278	55582-55584	,	denotes	,
T12279	55584-55589	NNP	denotes	Texas
T12280	55590-55593	NNP	denotes	Red
T12281	55593-55594	HYPH	denotes	-
T12282	55594-55596	,	denotes	,
T12283	55596-55598	CC	denotes	or
T12284	55599-55602	NN	denotes	HRP
T12285	55602-55603	HYPH	denotes	-
T12276	55603-55613	VBN	denotes	conjugated
T12287	55614-55623	JJ	denotes	secondary
T12286	55624-55634	NNS	denotes	antibodies
T12288	55635-55639	VBD	denotes	were
T12289	55640-55644	IN	denotes	from
T12290	55645-55652	NNP	denotes	Jackson
T12291	55653-55667	NNP	denotes	ImmunoResearch
T12292	55668-55669	-LRB-	denotes	(
T12294	55669-55673	NNP	denotes	West
T12293	55674-55679	NNP	denotes	Grove
T12295	55679-55681	,	denotes	,
T12296	55681-55693	NNP	denotes	Pennsylvania
T12297	55693-55695	,	denotes	,
T12298	55695-55701	NNP	denotes	United
T12299	55702-55708	NNP	denotes	States
T12300	55708-55709	-RRB-	denotes	)
T12301	55709-55710	.	denotes	.
T12302	55710-55807	sentence	denotes	Biotinylated secondary antibodies were from Vector Labs (Burlingame, California, United States).
T12303	55711-55723	VBN	denotes	Biotinylated
T12305	55724-55733	JJ	denotes	secondary
T12304	55734-55744	NNS	denotes	antibodies
T12306	55745-55749	VBD	denotes	were
T12307	55750-55754	IN	denotes	from
T12308	55755-55761	NNP	denotes	Vector
T12309	55762-55766	NNP	denotes	Labs
T12310	55767-55768	-LRB-	denotes	(
T12311	55768-55778	NNP	denotes	Burlingame
T12312	55778-55780	,	denotes	,
T12313	55780-55790	NNP	denotes	California
T12314	55790-55792	,	denotes	,
T12315	55792-55798	NNP	denotes	United
T12316	55799-55805	NNP	denotes	States
T12317	55805-55806	-RRB-	denotes	)
T12318	55806-55807	.	denotes	.
T12319	55807-55870	sentence	denotes	Dilutions were according to the manufacturer's recommendation.
T12320	55808-55817	NNS	denotes	Dilutions
T12321	55818-55822	VBD	denotes	were
T12322	55823-55832	VBG	denotes	according
T12323	55833-55835	IN	denotes	to
T12324	55836-55839	DT	denotes	the
T12325	55840-55852	NN	denotes	manufacturer
T12327	55852-55854	POS	denotes	's
T12326	55855-55869	NN	denotes	recommendation
T12328	55869-55870	.	denotes	.
T12329	55870-56045	sentence	denotes	The Snail antibody was generated in Guinea pigs by inoculating them with the N-terminal sequence of murine Snail fused to GST (Covance, Princeton, New Jersey, United States).
T12330	55871-55874	DT	denotes	The
T12332	55875-55880	NN	denotes	Snail
T12331	55881-55889	NN	denotes	antibody
T12334	55890-55893	VBD	denotes	was
T12333	55894-55903	VBN	denotes	generated
T12335	55904-55906	IN	denotes	in
T12336	55907-55913	NN	denotes	Guinea
T12337	55914-55918	NNS	denotes	pigs
T12338	55919-55921	IN	denotes	by
T12339	55922-55933	VBG	denotes	inoculating
T12340	55934-55938	PRP	denotes	them
T12341	55939-55943	IN	denotes	with
T12342	55944-55947	DT	denotes	the
T12344	55948-55949	NN	denotes	N
T12346	55949-55950	HYPH	denotes	-
T12345	55950-55958	JJ	denotes	terminal
T12343	55959-55967	NN	denotes	sequence
T12347	55968-55970	IN	denotes	of
T12348	55971-55977	JJ	denotes	murine
T12349	55978-55983	NN	denotes	Snail
T12350	55984-55989	VBN	denotes	fused
T12351	55990-55992	IN	denotes	to
T12352	55993-55996	NN	denotes	GST
T12353	55997-55998	-LRB-	denotes	(
T12354	55998-56005	NNP	denotes	Covance
T12355	56005-56007	,	denotes	,
T12356	56007-56016	NNP	denotes	Princeton
T12357	56016-56018	,	denotes	,
T12358	56018-56021	NNP	denotes	New
T12359	56022-56028	NNP	denotes	Jersey
T12360	56028-56030	,	denotes	,
T12361	56030-56036	NNP	denotes	United
T12362	56037-56043	NNP	denotes	States
T12363	56043-56044	-RRB-	denotes	)
T12364	56044-56045	.	denotes	.
T12365	56045-56134	sentence	denotes	Recombinant human TGF-β2 was purchased from R&D (Minneapolis, Minnesota, United States).
T12366	56046-56057	JJ	denotes	Recombinant
T12368	56058-56063	JJ	denotes	human
T12369	56064-56067	NN	denotes	TGF
T12370	56067-56068	HYPH	denotes	-
T12367	56068-56070	NN	denotes	β2
T12372	56071-56074	VBD	denotes	was
T12371	56075-56084	VBN	denotes	purchased
T12373	56085-56089	IN	denotes	from
T12374	56090-56091	NNP	denotes	R
T12375	56091-56092	CC	denotes	&
T12376	56092-56093	NNP	denotes	D
T12377	56094-56095	-LRB-	denotes	(
T12378	56095-56106	NNP	denotes	Minneapolis
T12379	56106-56108	,	denotes	,
T12380	56108-56117	NNP	denotes	Minnesota
T12381	56117-56119	,	denotes	,
T12382	56119-56125	NNP	denotes	United
T12383	56126-56132	NNP	denotes	States
T12384	56132-56133	-RRB-	denotes	)
T12385	56133-56134	.	denotes	.
T12386	56134-56229	sentence	denotes	Heat inactivated TGF-β2 was generated by heating the recombinant protein at 100 °C for 10 min.
T12387	56135-56139	NN	denotes	Heat
T12388	56140-56151	VBN	denotes	inactivated
T12390	56152-56155	NN	denotes	TGF
T12391	56155-56156	HYPH	denotes	-
T12389	56156-56158	NN	denotes	β2
T12393	56159-56162	VBD	denotes	was
T12392	56163-56172	VBN	denotes	generated
T12394	56173-56175	IN	denotes	by
T12395	56176-56183	VBG	denotes	heating
T12396	56184-56187	DT	denotes	the
T12398	56188-56199	JJ	denotes	recombinant
T12397	56200-56207	NN	denotes	protein
T12399	56208-56210	IN	denotes	at
T12400	56211-56214	CD	denotes	100
T12401	56215-56217	NN	denotes	°C
T12402	56218-56221	IN	denotes	for
T12403	56222-56224	CD	denotes	10
T12404	56225-56228	NN	denotes	min
T12405	56228-56229	.	denotes	.
T12464	56231-56235	NNS	denotes	Mice
T12465	56235-56438	sentence	denotes	The K14-Snail Tg mouse was generated by digesting the pcDNA3-mm Snail-HA plasmid (G. de Herreros, Universitat Pompeu, Fabra, Barcelona, Spain) with BamHI and NotI and subcloned into the K14 vector [49].
T12466	56236-56239	DT	denotes	The
T12468	56240-56243	NN	denotes	K14
T12470	56243-56244	HYPH	denotes	-
T12469	56244-56249	NN	denotes	Snail
T12471	56250-56252	NN	denotes	Tg
T12467	56253-56258	NN	denotes	mouse
T12473	56259-56262	VBD	denotes	was
T12472	56263-56272	VBN	denotes	generated
T12474	56273-56275	IN	denotes	by
T12475	56276-56285	VBG	denotes	digesting
T12476	56286-56289	DT	denotes	the
T12478	56290-56296	NN	denotes	pcDNA3
T12480	56296-56297	HYPH	denotes	-
T12479	56297-56299	NN	denotes	mm
T12481	56300-56305	NN	denotes	Snail
T12483	56305-56306	HYPH	denotes	-
T12482	56306-56308	NN	denotes	HA
T12477	56309-56316	NN	denotes	plasmid
T12484	56317-56318	-LRB-	denotes	(
T12486	56318-56320	NNP	denotes	G.
T12487	56321-56323	NNP	denotes	de
T12485	56324-56332	NNP	denotes	Herreros
T12488	56332-56334	,	denotes	,
T12489	56334-56345	NNP	denotes	Universitat
T12490	56346-56352	NNP	denotes	Pompeu
T12491	56352-56354	,	denotes	,
T12492	56354-56359	NNP	denotes	Fabra
T12493	56359-56361	,	denotes	,
T12494	56361-56370	NNP	denotes	Barcelona
T12495	56370-56372	,	denotes	,
T12496	56372-56377	NNP	denotes	Spain
T12497	56377-56378	-RRB-	denotes	)
T12498	56379-56383	IN	denotes	with
T12499	56384-56389	NN	denotes	BamHI
T12500	56390-56393	CC	denotes	and
T12501	56394-56398	NN	denotes	NotI
T12502	56399-56402	CC	denotes	and
T12503	56403-56412	VBN	denotes	subcloned
T12504	56413-56417	IN	denotes	into
T12505	56418-56421	DT	denotes	the
T12507	56422-56425	NN	denotes	K14
T12506	56426-56432	NN	denotes	vector
T12508	56433-56434	-LRB-	denotes	[
T12509	56434-56436	CD	denotes	49
T12510	56436-56437	-RRB-	denotes	]
T12511	56437-56438	.	denotes	.
T12512	56438-56519	sentence	denotes	The linearized construct was injected into the nucleus of embryos from CD1 mice.
T12513	56439-56442	DT	denotes	The
T12515	56443-56453	VBN	denotes	linearized
T12514	56454-56463	NN	denotes	construct
T12517	56464-56467	VBD	denotes	was
T12516	56468-56476	VBN	denotes	injected
T12518	56477-56481	IN	denotes	into
T12519	56482-56485	DT	denotes	the
T12520	56486-56493	NN	denotes	nucleus
T12521	56494-56496	IN	denotes	of
T12522	56497-56504	NNS	denotes	embryos
T12523	56505-56509	IN	denotes	from
T12524	56510-56513	NN	denotes	CD1
T12525	56514-56518	NNS	denotes	mice
T12526	56518-56519	.	denotes	.
T12527	56519-56577	sentence	denotes	The K14-Smad 2 Tg mouse was reported in Ito et al., 2001.
T12528	56520-56523	DT	denotes	The
T12530	56524-56527	NN	denotes	K14
T12532	56527-56528	HYPH	denotes	-
T12531	56528-56532	NN	denotes	Smad
T12533	56533-56534	CD	denotes	2
T12534	56535-56537	NN	denotes	Tg
T12529	56538-56543	NN	denotes	mouse
T12536	56544-56547	VBD	denotes	was
T12535	56548-56556	VBN	denotes	reported
T12537	56557-56559	IN	denotes	in
T12538	56560-56563	NNP	denotes	Ito
T12539	56564-56566	FW	denotes	et
T12540	56567-56570	FW	denotes	al.
T12541	56570-56572	,	denotes	,
T12542	56572-56576	CD	denotes	2001
T12543	56576-56577	.	denotes	.
T12544	56577-56631	sentence	denotes	The TGF-β2 knockout (KO) mouse was described in [34].
T12545	56578-56581	DT	denotes	The
T12547	56582-56585	NN	denotes	TGF
T12549	56585-56586	HYPH	denotes	-
T12548	56586-56588	NN	denotes	β2
T12550	56589-56597	NN	denotes	knockout
T12551	56598-56599	-LRB-	denotes	(
T12552	56599-56601	NN	denotes	KO
T12553	56601-56602	-RRB-	denotes	)
T12546	56603-56608	NN	denotes	mouse
T12555	56609-56612	VBD	denotes	was
T12554	56613-56622	VBN	denotes	described
T12556	56623-56625	IN	denotes	in
T12557	56626-56627	-LRB-	denotes	[
T12558	56627-56629	NN	denotes	34
T12559	56629-56630	-RRB-	denotes	]
T12560	56630-56631	.	denotes	.
T12561	56631-56706	sentence	denotes	The shh KO mouse [38] and TOPGal mouse [20] have previously been reported.
T12562	56632-56635	DT	denotes	The
T12564	56636-56639	NN	denotes	shh
T12565	56640-56642	NN	denotes	KO
T12563	56643-56648	NN	denotes	mouse
T12567	56649-56650	-LRB-	denotes	[
T12568	56650-56652	CD	denotes	38
T12569	56652-56653	-RRB-	denotes	]
T12570	56654-56657	CC	denotes	and
T12571	56658-56664	NN	denotes	TOPGal
T12572	56665-56670	NN	denotes	mouse
T12573	56671-56672	-LRB-	denotes	[
T12574	56672-56674	CD	denotes	20
T12575	56674-56675	-RRB-	denotes	]
T12576	56676-56680	VBP	denotes	have
T12577	56681-56691	RB	denotes	previously
T12578	56692-56696	VBN	denotes	been
T12566	56697-56705	VBN	denotes	reported
T12579	56705-56706	.	denotes	.
T12687	56708-56715	NNP	denotes	Western
T12688	56716-56720	NN	denotes	blot
T12689	56721-56724	CC	denotes	and
T12690	56725-56744	NN	denotes	immunoprecipitation
T12691	56744-57050	sentence	denotes	Protein extracts from primary keratinocytes were generated either by lysing cells in lysis buffer (1% NP-40, 1% sodium deoxycholate, 20 mM Tris-Cl [pH 7.4], 140 mM NaCl containing 1 mM sodium vanadate, 2 mM phenylmethylsulfonyl fluoride, and protease inhibitors) or directly in Laemmli bβuffer and boiled.
T12692	56745-56752	NN	denotes	Protein
T12693	56753-56761	NNS	denotes	extracts
T12695	56762-56766	IN	denotes	from
T12696	56767-56774	JJ	denotes	primary
T12697	56775-56788	NNS	denotes	keratinocytes
T12698	56789-56793	VBD	denotes	were
T12694	56794-56803	VBN	denotes	generated
T12699	56804-56810	CC	denotes	either
T12700	56811-56813	IN	denotes	by
T12701	56814-56820	VBG	denotes	lysing
T12702	56821-56826	NNS	denotes	cells
T12703	56827-56829	IN	denotes	in
T12704	56830-56835	NN	denotes	lysis
T12705	56836-56842	NN	denotes	buffer
T12706	56843-56844	-LRB-	denotes	(
T12708	56844-56845	CD	denotes	1
T12709	56845-56846	NN	denotes	%
T12707	56847-56849	NN	denotes	NP
T12710	56849-56850	HYPH	denotes	-
T12711	56850-56852	CD	denotes	40
T12712	56852-56854	,	denotes	,
T12713	56854-56855	CD	denotes	1
T12714	56855-56856	NN	denotes	%
T12716	56857-56863	NN	denotes	sodium
T12715	56864-56876	NN	denotes	deoxycholate
T12717	56876-56878	,	denotes	,
T12718	56878-56880	CD	denotes	20
T12719	56881-56883	NN	denotes	mM
T12721	56884-56888	NN	denotes	Tris
T12722	56888-56889	HYPH	denotes	-
T12720	56889-56891	NN	denotes	Cl
T12723	56892-56893	-LRB-	denotes	[
T12724	56893-56895	NN	denotes	pH
T12725	56896-56899	CD	denotes	7.4
T12726	56899-56900	-RRB-	denotes	]
T12727	56900-56902	,	denotes	,
T12728	56902-56905	CD	denotes	140
T12729	56906-56908	NN	denotes	mM
T12730	56909-56913	NN	denotes	NaCl
T12731	56914-56924	VBG	denotes	containing
T12732	56925-56926	CD	denotes	1
T12733	56927-56929	NN	denotes	mM
T12735	56930-56936	NN	denotes	sodium
T12734	56937-56945	NN	denotes	vanadate
T12736	56945-56947	,	denotes	,
T12737	56947-56948	CD	denotes	2
T12738	56949-56951	NN	denotes	mM
T12740	56952-56972	NN	denotes	phenylmethylsulfonyl
T12739	56973-56981	NN	denotes	fluoride
T12741	56981-56983	,	denotes	,
T12742	56983-56986	CC	denotes	and
T12743	56987-56995	NN	denotes	protease
T12744	56996-57006	NNS	denotes	inhibitors
T12745	57006-57007	-RRB-	denotes	)
T12746	57008-57010	CC	denotes	or
T12747	57011-57019	RB	denotes	directly
T12748	57020-57022	IN	denotes	in
T12749	57023-57030	NNP	denotes	Laemmli
T12750	57031-57038	NN	denotes	bβuffer
T12751	57039-57042	CC	denotes	and
T12752	57043-57049	VBN	denotes	boiled
T12753	57049-57050	.	denotes	.
T12754	57050-57199	sentence	denotes	For skin tissue: Frozen tissue was pulverized in a liquid nitrogen-cooled Gevebesmascher and the powder scraped into a chilled microcentrifuge tube.
T12755	57051-57054	IN	denotes	For
T12757	57055-57059	NN	denotes	skin
T12758	57060-57066	NN	denotes	tissue
T12759	57066-57068	:	denotes	:
T12760	57068-57074	JJ	denotes	Frozen
T12761	57075-57081	NN	denotes	tissue
T12762	57082-57085	VBD	denotes	was
T12756	57086-57096	VBN	denotes	pulverized
T12763	57097-57099	IN	denotes	in
T12764	57100-57101	DT	denotes	a
T12766	57102-57108	JJ	denotes	liquid
T12767	57109-57117	NN	denotes	nitrogen
T12769	57117-57118	HYPH	denotes	-
T12768	57118-57124	VBN	denotes	cooled
T12765	57125-57139	NN	denotes	Gevebesmascher
T12770	57140-57143	CC	denotes	and
T12771	57144-57147	DT	denotes	the
T12772	57148-57154	NN	denotes	powder
T12773	57155-57162	VBN	denotes	scraped
T12774	57163-57167	IN	denotes	into
T12775	57168-57169	DT	denotes	a
T12777	57170-57177	JJ	denotes	chilled
T12778	57178-57193	NN	denotes	microcentrifuge
T12776	57194-57198	NN	denotes	tube
T12779	57198-57199	.	denotes	.
T12780	57199-57356	sentence	denotes	RIPA buffer (1% Triton X-100 in PBS with 10 mM EDTA, 150 mN NaCl, 1% sodium deoxycholate, and 0.1% SDS) and protease inhibitors or Laemmli buffer was added.
T12781	57200-57204	NN	denotes	RIPA
T12782	57205-57211	NN	denotes	buffer
T12784	57212-57213	-LRB-	denotes	(
T12786	57213-57214	CD	denotes	1
T12787	57214-57215	NN	denotes	%
T12788	57216-57222	NN	denotes	Triton
T12785	57223-57224	NN	denotes	X
T12789	57224-57225	HYPH	denotes	-
T12790	57225-57228	CD	denotes	100
T12791	57229-57231	IN	denotes	in
T12792	57232-57235	NN	denotes	PBS
T12793	57236-57240	IN	denotes	with
T12794	57241-57243	CD	denotes	10
T12795	57244-57246	NN	denotes	mM
T12796	57247-57251	NN	denotes	EDTA
T12797	57251-57253	,	denotes	,
T12798	57253-57256	CD	denotes	150
T12799	57257-57259	NN	denotes	mN
T12800	57260-57264	NN	denotes	NaCl
T12801	57264-57266	,	denotes	,
T12802	57266-57267	CD	denotes	1
T12803	57267-57268	NN	denotes	%
T12805	57269-57275	NN	denotes	sodium
T12804	57276-57288	NN	denotes	deoxycholate
T12806	57288-57290	,	denotes	,
T12807	57290-57293	CC	denotes	and
T12808	57294-57297	CD	denotes	0.1
T12809	57297-57298	NN	denotes	%
T12810	57299-57302	NN	denotes	SDS
T12811	57302-57303	-RRB-	denotes	)
T12812	57304-57307	CC	denotes	and
T12813	57308-57316	NN	denotes	protease
T12814	57317-57327	NNS	denotes	inhibitors
T12815	57328-57330	CC	denotes	or
T12816	57331-57338	NNP	denotes	Laemmli
T12817	57339-57345	NN	denotes	buffer
T12818	57346-57349	VBD	denotes	was
T12783	57350-57355	VBN	denotes	added
T12819	57355-57356	.	denotes	.
T12820	57356-57450	sentence	denotes	The cell suspension was sonicated three times for 15 s and centrifuged at 14,000 rpm at 4 °C.
T12821	57357-57360	DT	denotes	The
T12823	57361-57365	NN	denotes	cell
T12822	57366-57376	NN	denotes	suspension
T12825	57377-57380	VBD	denotes	was
T12824	57381-57390	VBN	denotes	sonicated
T12826	57391-57396	CD	denotes	three
T12827	57397-57402	NNS	denotes	times
T12828	57403-57406	IN	denotes	for
T12829	57407-57409	CD	denotes	15
T12830	57410-57411	NN	denotes	s
T12831	57412-57415	CC	denotes	and
T12832	57416-57427	VBN	denotes	centrifuged
T12833	57428-57430	IN	denotes	at
T12834	57431-57437	CD	denotes	14,000
T12835	57438-57441	NN	denotes	rpm
T12836	57442-57444	IN	denotes	at
T12837	57445-57446	CD	denotes	4
T12838	57447-57449	NN	denotes	°C
T12839	57449-57450	.	denotes	.
T12840	57450-57525	sentence	denotes	The supernatant was separated from the pellet and used in the experiments.
T12841	57451-57454	DT	denotes	The
T12842	57455-57466	NN	denotes	supernatant
T12844	57467-57470	VBD	denotes	was
T12843	57471-57480	VBN	denotes	separated
T12845	57481-57485	IN	denotes	from
T12846	57486-57489	DT	denotes	the
T12847	57490-57496	NN	denotes	pellet
T12848	57497-57500	CC	denotes	and
T12849	57501-57505	VBN	denotes	used
T12850	57506-57508	IN	denotes	in
T12851	57509-57512	DT	denotes	the
T12852	57513-57524	NNS	denotes	experiments
T12853	57524-57525	.	denotes	.
T12854	57525-57716	sentence	denotes	Extracts subjected to immunoprecipitation were precleared with Protein G Sepharose (Amersham, Piscataway, New York, United States) and incubated with antibody with rocking overnight at 4 °C.
T12855	57526-57534	NNS	denotes	Extracts
T12857	57535-57544	VBN	denotes	subjected
T12858	57545-57547	IN	denotes	to
T12859	57548-57567	NN	denotes	immunoprecipitation
T12860	57568-57572	VBD	denotes	were
T12856	57573-57583	VBN	denotes	precleared
T12861	57584-57588	IN	denotes	with
T12862	57589-57596	NN	denotes	Protein
T12863	57597-57598	NN	denotes	G
T12864	57599-57608	NN	denotes	Sepharose
T12865	57609-57610	-LRB-	denotes	(
T12866	57610-57618	NNP	denotes	Amersham
T12867	57618-57620	,	denotes	,
T12868	57620-57630	NNP	denotes	Piscataway
T12869	57630-57632	,	denotes	,
T12870	57632-57635	NNP	denotes	New
T12871	57636-57640	NNP	denotes	York
T12872	57640-57642	,	denotes	,
T12873	57642-57648	NNP	denotes	United
T12874	57649-57655	NNP	denotes	States
T12875	57655-57656	-RRB-	denotes	)
T12876	57657-57660	CC	denotes	and
T12877	57661-57670	VBN	denotes	incubated
T12878	57671-57675	IN	denotes	with
T12879	57676-57684	NN	denotes	antibody
T12880	57685-57689	IN	denotes	with
T12881	57690-57697	NN	denotes	rocking
T12882	57698-57707	RB	denotes	overnight
T12883	57708-57710	IN	denotes	at
T12884	57711-57712	CD	denotes	4
T12885	57713-57715	NN	denotes	°C
T12886	57715-57716	.	denotes	.
T12887	57716-57803	sentence	denotes	Protein G Sepharose was added and samples were incubated for 1 h at 4 °C with rocking.
T12888	57717-57724	NN	denotes	Protein
T12889	57725-57726	NN	denotes	G
T12890	57727-57736	NN	denotes	Sepharose
T12892	57737-57740	VBD	denotes	was
T12891	57741-57746	VBN	denotes	added
T12893	57747-57750	CC	denotes	and
T12894	57751-57758	NNS	denotes	samples
T12896	57759-57763	VBD	denotes	were
T12895	57764-57773	VBN	denotes	incubated
T12897	57774-57777	IN	denotes	for
T12898	57778-57779	CD	denotes	1
T12899	57780-57781	NN	denotes	h
T12900	57782-57784	IN	denotes	at
T12901	57785-57786	CD	denotes	4
T12902	57787-57789	NN	denotes	°C
T12903	57790-57794	IN	denotes	with
T12904	57795-57802	NN	denotes	rocking
T12905	57802-57803	.	denotes	.
T12906	57803-57976	sentence	denotes	Samples were washed three times for 5 min each in lysis buffer, and the Protein G Sepharose-antibody-antigen pellet was resuspended in Laemmli buffer and boiled for 10 min.
T12907	57804-57811	NNS	denotes	Samples
T12909	57812-57816	VBD	denotes	were
T12908	57817-57823	VBN	denotes	washed
T12910	57824-57829	CD	denotes	three
T12911	57830-57835	NNS	denotes	times
T12912	57836-57839	IN	denotes	for
T12913	57840-57841	CD	denotes	5
T12914	57842-57845	NN	denotes	min
T12915	57846-57850	RB	denotes	each
T12916	57851-57853	IN	denotes	in
T12917	57854-57859	NN	denotes	lysis
T12918	57860-57866	NN	denotes	buffer
T12919	57866-57868	,	denotes	,
T12920	57868-57871	CC	denotes	and
T12921	57872-57875	DT	denotes	the
T12923	57876-57883	NN	denotes	Protein
T12924	57884-57885	NN	denotes	G
T12926	57886-57895	NN	denotes	Sepharose
T12927	57895-57896	HYPH	denotes	-
T12928	57896-57904	NN	denotes	antibody
T12929	57904-57905	HYPH	denotes	-
T12925	57905-57912	NN	denotes	antigen
T12922	57913-57919	NN	denotes	pellet
T12931	57920-57923	VBD	denotes	was
T12930	57924-57935	VBN	denotes	resuspended
T12932	57936-57938	IN	denotes	in
T12933	57939-57946	NNP	denotes	Laemmli
T12934	57947-57953	NN	denotes	buffer
T12935	57954-57957	CC	denotes	and
T12936	57958-57964	VBN	denotes	boiled
T12937	57965-57968	IN	denotes	for
T12938	57969-57971	CD	denotes	10
T12939	57972-57975	NN	denotes	min
T12940	57975-57976	.	denotes	.
T12941	57976-58122	sentence	denotes	Samples were run on SDS-PAGE and transferred to nitrocellulose membrane (Schleicher and Schuell Bioscience, Keene, New Hampshire, United States).
T12942	57977-57984	NNS	denotes	Samples
T12944	57985-57989	VBD	denotes	were
T12943	57990-57993	VBN	denotes	run
T12945	57994-57996	IN	denotes	on
T12946	57997-58000	NN	denotes	SDS
T12948	58000-58001	HYPH	denotes	-
T12947	58001-58005	NN	denotes	PAGE
T12949	58006-58009	CC	denotes	and
T12950	58010-58021	VBN	denotes	transferred
T12951	58022-58024	IN	denotes	to
T12952	58025-58039	NN	denotes	nitrocellulose
T12953	58040-58048	NN	denotes	membrane
T12954	58049-58050	-LRB-	denotes	(
T12956	58050-58060	NNP	denotes	Schleicher
T12957	58061-58064	CC	denotes	and
T12958	58065-58072	NNP	denotes	Schuell
T12955	58073-58083	NNP	denotes	Bioscience
T12959	58083-58085	,	denotes	,
T12960	58085-58090	NNP	denotes	Keene
T12961	58090-58092	,	denotes	,
T12962	58092-58095	NNP	denotes	New
T12963	58096-58105	NNP	denotes	Hampshire
T12964	58105-58107	,	denotes	,
T12965	58107-58113	NNP	denotes	United
T12966	58114-58120	NNP	denotes	States
T12967	58120-58121	-RRB-	denotes	)
T12968	58121-58122	.	denotes	.
T12969	58122-58213	sentence	denotes	Western blot signals were developed using the enhanced chemiluminescence kit from Amersham
T12970	58123-58130	NNP	denotes	Western
T12971	58131-58135	NN	denotes	blot
T12972	58136-58143	NNS	denotes	signals
T12974	58144-58148	VBD	denotes	were
T12973	58149-58158	VBN	denotes	developed
T12975	58159-58164	VBG	denotes	using
T12976	58165-58168	DT	denotes	the
T12978	58169-58177	VBN	denotes	enhanced
T12979	58178-58195	NN	denotes	chemiluminescence
T12977	58196-58199	NN	denotes	kit
T12980	58200-58204	IN	denotes	from
T12981	58205-58213	NNP	denotes	Amersham
T13041	58215-58219	NN	denotes	Cell
T13042	58220-58227	NN	denotes	culture
T13043	58227-58313	sentence	denotes	Primary keratinocytes were culture in low-calcium medium as previously described [4].
T13044	58228-58235	JJ	denotes	Primary
T13045	58236-58249	NNS	denotes	keratinocytes
T13047	58250-58254	VBD	denotes	were
T13046	58255-58262	VBN	denotes	culture
T13048	58263-58265	IN	denotes	in
T13049	58266-58269	JJ	denotes	low
T13051	58269-58270	HYPH	denotes	-
T13050	58270-58277	NN	denotes	calcium
T13052	58278-58284	NN	denotes	medium
T13053	58285-58287	IN	denotes	as
T13055	58288-58298	RB	denotes	previously
T13054	58299-58308	VBN	denotes	described
T13056	58309-58310	-LRB-	denotes	[
T13057	58310-58311	CD	denotes	4
T13058	58311-58312	-RRB-	denotes	]
T13059	58312-58313	.	denotes	.
T13060	58313-58463	sentence	denotes	Transient transfections were carried out with FuGENE6 reagent (Roche, Indianapolis, Indiana, United States) according to the manufacturer's protocol.
T13061	58314-58323	JJ	denotes	Transient
T13062	58324-58337	NNS	denotes	transfections
T13064	58338-58342	VBD	denotes	were
T13063	58343-58350	VBN	denotes	carried
T13065	58351-58354	RP	denotes	out
T13066	58355-58359	IN	denotes	with
T13067	58360-58367	NN	denotes	FuGENE6
T13068	58368-58375	NN	denotes	reagent
T13069	58376-58377	-LRB-	denotes	(
T13070	58377-58382	NNP	denotes	Roche
T13071	58382-58384	,	denotes	,
T13072	58384-58396	NNP	denotes	Indianapolis
T13073	58396-58398	,	denotes	,
T13074	58398-58405	NNP	denotes	Indiana
T13075	58405-58407	,	denotes	,
T13076	58407-58413	NNP	denotes	United
T13077	58414-58420	NNP	denotes	States
T13078	58420-58421	-RRB-	denotes	)
T13079	58422-58431	VBG	denotes	according
T13080	58432-58434	IN	denotes	to
T13081	58435-58438	DT	denotes	the
T13082	58439-58451	NN	denotes	manufacturer
T13084	58451-58453	POS	denotes	's
T13083	58454-58462	NN	denotes	protocol
T13085	58462-58463	.	denotes	.
T13086	58463-58725	sentence	denotes	Measurement of β-galactosidase or luciferase levels in promoter activity studies were carried out with the Galacto-Lite assay kit (TROPIX, Bedford, Massachusetts, United States) and the Dual luciferase (Promega, Madison, Wisconsin, United States), respectively.
T13087	58464-58475	NN	denotes	Measurement
T13089	58476-58478	IN	denotes	of
T13090	58479-58480	NN	denotes	β
T13092	58480-58481	HYPH	denotes	-
T13091	58481-58494	NN	denotes	galactosidase
T13094	58495-58497	CC	denotes	or
T13095	58498-58508	NN	denotes	luciferase
T13093	58509-58515	NNS	denotes	levels
T13096	58516-58518	IN	denotes	in
T13097	58519-58527	NN	denotes	promoter
T13098	58528-58536	NN	denotes	activity
T13099	58537-58544	NNS	denotes	studies
T13100	58545-58549	VBD	denotes	were
T13088	58550-58557	VBN	denotes	carried
T13101	58558-58561	RP	denotes	out
T13102	58562-58566	IN	denotes	with
T13103	58567-58570	DT	denotes	the
T13105	58571-58578	NNP	denotes	Galacto
T13107	58578-58579	HYPH	denotes	-
T13106	58579-58583	NNP	denotes	Lite
T13108	58584-58589	NN	denotes	assay
T13104	58590-58593	NN	denotes	kit
T13109	58594-58595	-LRB-	denotes	(
T13110	58595-58601	NNP	denotes	TROPIX
T13111	58601-58603	,	denotes	,
T13112	58603-58610	NNP	denotes	Bedford
T13113	58610-58612	,	denotes	,
T13114	58612-58625	NNP	denotes	Massachusetts
T13115	58625-58627	,	denotes	,
T13116	58627-58633	NNP	denotes	United
T13117	58634-58640	NNP	denotes	States
T13118	58640-58641	-RRB-	denotes	)
T13119	58642-58645	CC	denotes	and
T13120	58646-58649	DT	denotes	the
T13122	58650-58654	JJ	denotes	Dual
T13121	58655-58665	NN	denotes	luciferase
T13123	58666-58667	-LRB-	denotes	(
T13124	58667-58674	NNP	denotes	Promega
T13125	58674-58676	,	denotes	,
T13126	58676-58683	NNP	denotes	Madison
T13127	58683-58685	,	denotes	,
T13128	58685-58694	NNP	denotes	Wisconsin
T13129	58694-58696	,	denotes	,
T13130	58696-58702	NNP	denotes	United
T13131	58703-58709	NNP	denotes	States
T13132	58709-58710	-RRB-	denotes	)
T13133	58710-58712	,	denotes	,
T13134	58712-58724	RB	denotes	respectively
T13135	58724-58725	.	denotes	.
T13136	58725-58813	sentence	denotes	Runella luciferase was cotransfected into cells to correct for transfection efficiency.
T13137	58726-58733	NN	denotes	Runella
T13138	58734-58744	NN	denotes	luciferase
T13140	58745-58748	VBD	denotes	was
T13139	58749-58762	VBN	denotes	cotransfected
T13141	58763-58767	IN	denotes	into
T13142	58768-58773	NNS	denotes	cells
T13143	58774-58776	TO	denotes	to
T13144	58777-58784	VB	denotes	correct
T13145	58785-58788	IN	denotes	for
T13146	58789-58801	NN	denotes	transfection
T13147	58802-58812	NN	denotes	efficiency
T13148	58812-58813	.	denotes	.
T13149	58813-58884	sentence	denotes	Experiments were done in triplicate and repeated at least three times.
T13150	58814-58825	NNS	denotes	Experiments
T13152	58826-58830	VBD	denotes	were
T13151	58831-58835	VBN	denotes	done
T13153	58836-58838	IN	denotes	in
T13154	58839-58849	NN	denotes	triplicate
T13155	58850-58853	CC	denotes	and
T13156	58854-58862	VBN	denotes	repeated
T13157	58863-58865	RB	denotes	at
T13159	58866-58871	RBS	denotes	least
T13158	58872-58877	CD	denotes	three
T13160	58878-58883	NNS	denotes	times
T13161	58883-58884	.	denotes	.
T13162	58884-58979	sentence	denotes	Measurements were done on a luminometer (MGM Instruments, Hamden, Connecticut, United States).
T13163	58885-58897	NNS	denotes	Measurements
T13165	58898-58902	VBD	denotes	were
T13164	58903-58907	VBN	denotes	done
T13166	58908-58910	IN	denotes	on
T13167	58911-58912	DT	denotes	a
T13168	58913-58924	NN	denotes	luminometer
T13169	58925-58926	-LRB-	denotes	(
T13171	58926-58929	NNP	denotes	MGM
T13170	58930-58941	NNP	denotes	Instruments
T13172	58941-58943	,	denotes	,
T13173	58943-58949	NNP	denotes	Hamden
T13174	58949-58951	,	denotes	,
T13175	58951-58962	NNP	denotes	Connecticut
T13176	58962-58964	,	denotes	,
T13177	58964-58970	NNP	denotes	United
T13178	58971-58977	NNP	denotes	States
T13179	58977-58978	-RRB-	denotes	)
T13180	58978-58979	.	denotes	.
T13181	58979-59139	sentence	denotes	For experiments measuring phosphorylation of MAPK, keratinocytes were serum starved for 3 h prior to harvesting of cells by incubation in medium lacking serum.
T13182	58980-58983	IN	denotes	For
T13184	58984-58995	NNS	denotes	experiments
T13185	58996-59005	VBG	denotes	measuring
T13186	59006-59021	NN	denotes	phosphorylation
T13187	59022-59024	IN	denotes	of
T13188	59025-59029	NN	denotes	MAPK
T13189	59029-59031	,	denotes	,
T13190	59031-59044	NNS	denotes	keratinocytes
T13191	59045-59049	VBD	denotes	were
T13192	59050-59055	NN	denotes	serum
T13183	59056-59063	VBN	denotes	starved
T13193	59064-59067	IN	denotes	for
T13194	59068-59069	CD	denotes	3
T13195	59070-59071	NN	denotes	h
T13196	59072-59077	JJ	denotes	prior
T13197	59078-59080	IN	denotes	to
T13198	59081-59091	NN	denotes	harvesting
T13199	59092-59094	IN	denotes	of
T13200	59095-59100	NNS	denotes	cells
T13201	59101-59103	IN	denotes	by
T13202	59104-59114	NN	denotes	incubation
T13203	59115-59117	IN	denotes	in
T13204	59118-59124	NN	denotes	medium
T13205	59125-59132	VBG	denotes	lacking
T13206	59133-59138	NN	denotes	serum
T13207	59138-59139	.	denotes	.
T13208	59139-59228	sentence	denotes	Treatment of cells with Wnt- and noggin-conditioned medium was previously described [4].
T13209	59140-59149	NN	denotes	Treatment
T13211	59150-59152	IN	denotes	of
T13212	59153-59158	NNS	denotes	cells
T13213	59159-59163	IN	denotes	with
T13214	59164-59167	NN	denotes	Wnt
T13216	59167-59168	HYPH	denotes	-
T13217	59169-59172	CC	denotes	and
T13218	59173-59179	NN	denotes	noggin
T13219	59179-59180	HYPH	denotes	-
T13215	59180-59191	VBN	denotes	conditioned
T13220	59192-59198	NN	denotes	medium
T13221	59199-59202	VBD	denotes	was
T13222	59203-59213	RB	denotes	previously
T13210	59214-59223	VBN	denotes	described
T13223	59224-59225	-LRB-	denotes	[
T13224	59225-59226	CD	denotes	4
T13225	59226-59227	-RRB-	denotes	]
T13226	59227-59228	.	denotes	.
T13308	59230-59240	NNS	denotes	Constructs
T13309	59240-59521	sentence	denotes	The 2.2-kb murine Snail promoter was generated by PCR using a forward primer with an XbaI linker sequence, 5′- TCTAGAATTGTTTGCTGCTGTATGGTCTTC-3′, along with a reverse primer with a BglII linker sequence, 5′- AGATCTGTTGGCCAGAGCGACCTAG- GTAG-3′, and mouse genomic DNA as a template.
T13310	59241-59244	DT	denotes	The
T13312	59245-59248	CD	denotes	2.2
T13314	59248-59249	HYPH	denotes	-
T13313	59249-59251	NN	denotes	kb
T13315	59252-59258	JJ	denotes	murine
T13316	59259-59264	NN	denotes	Snail
T13311	59265-59273	NN	denotes	promoter
T13318	59274-59277	VBD	denotes	was
T13317	59278-59287	VBN	denotes	generated
T13319	59288-59290	IN	denotes	by
T13320	59291-59294	NN	denotes	PCR
T13321	59295-59300	VBG	denotes	using
T13322	59301-59302	DT	denotes	a
T13324	59303-59310	JJ	denotes	forward
T13323	59311-59317	NN	denotes	primer
T13325	59318-59322	IN	denotes	with
T13326	59323-59325	DT	denotes	an
T13328	59326-59330	NN	denotes	XbaI
T13329	59331-59337	NN	denotes	linker
T13327	59338-59346	NN	denotes	sequence
T13330	59346-59348	,	denotes	,
T13331	59348-59349	CD	denotes	5
T13333	59349-59350	SYM	denotes	′
T13334	59350-59351	HYPH	denotes	-
T13332	59352-59382	NN	denotes	TCTAGAATTGTTTGCTGCTGTATGGTCTTC
T13335	59382-59383	HYPH	denotes	-
T13336	59383-59384	CD	denotes	3
T13337	59384-59385	SYM	denotes	′
T13338	59385-59387	,	denotes	,
T13339	59387-59392	IN	denotes	along
T13340	59393-59397	IN	denotes	with
T13341	59398-59399	DT	denotes	a
T13343	59400-59407	JJ	denotes	reverse
T13342	59408-59414	NN	denotes	primer
T13344	59415-59419	IN	denotes	with
T13345	59420-59421	DT	denotes	a
T13347	59422-59427	NN	denotes	BglII
T13348	59428-59434	NN	denotes	linker
T13346	59435-59443	NN	denotes	sequence
T13349	59443-59445	,	denotes	,
T13350	59445-59446	CD	denotes	5
T13352	59446-59447	SYM	denotes	′
T13353	59447-59448	HYPH	denotes	-
T13354	59449-59474	NN	denotes	AGATCTGTTGGCCAGAGCGACCTAG
T13355	59474-59475	HYPH	denotes	-
T13351	59476-59480	NN	denotes	GTAG
T13356	59480-59481	HYPH	denotes	-
T13357	59481-59482	CD	denotes	3
T13358	59482-59483	SYM	denotes	′
T13359	59483-59485	,	denotes	,
T13360	59485-59488	CC	denotes	and
T13361	59489-59494	NN	denotes	mouse
T13363	59495-59502	JJ	denotes	genomic
T13362	59503-59506	NN	denotes	DNA
T13364	59507-59509	IN	denotes	as
T13365	59510-59511	DT	denotes	a
T13366	59512-59520	NN	denotes	template
T13367	59520-59521	.	denotes	.
T13368	59521-59713	sentence	denotes	The PCR product was purified with the Gel Extraction Kit (Qiagen, Valencia, California, United States) and ligated into pCRII-TOPO TA vector (Invitrogen, Carlsbad, California, United States).
T13369	59522-59525	DT	denotes	The
T13371	59526-59529	NN	denotes	PCR
T13370	59530-59537	NN	denotes	product
T13373	59538-59541	VBD	denotes	was
T13372	59542-59550	VBN	denotes	purified
T13374	59551-59555	IN	denotes	with
T13375	59556-59559	DT	denotes	the
T13377	59560-59563	NN	denotes	Gel
T13378	59564-59574	NN	denotes	Extraction
T13376	59575-59578	NN	denotes	Kit
T13379	59579-59580	-LRB-	denotes	(
T13380	59580-59586	NNP	denotes	Qiagen
T13381	59586-59588	,	denotes	,
T13382	59588-59596	NNP	denotes	Valencia
T13383	59596-59598	,	denotes	,
T13384	59598-59608	NNP	denotes	California
T13385	59608-59610	,	denotes	,
T13386	59610-59616	NNP	denotes	United
T13387	59617-59623	NNP	denotes	States
T13388	59623-59624	-RRB-	denotes	)
T13389	59625-59628	CC	denotes	and
T13390	59629-59636	VBN	denotes	ligated
T13391	59637-59641	IN	denotes	into
T13392	59642-59647	NN	denotes	pCRII
T13394	59647-59648	HYPH	denotes	-
T13393	59648-59652	NN	denotes	TOPO
T13396	59653-59655	NN	denotes	TA
T13395	59656-59662	NN	denotes	vector
T13397	59663-59664	-LRB-	denotes	(
T13398	59664-59674	NNP	denotes	Invitrogen
T13399	59674-59676	,	denotes	,
T13400	59676-59684	NNP	denotes	Carlsbad
T13401	59684-59686	,	denotes	,
T13402	59686-59696	NNP	denotes	California
T13403	59696-59698	,	denotes	,
T13404	59698-59704	NNP	denotes	United
T13405	59705-59711	NNP	denotes	States
T13406	59711-59712	-RRB-	denotes	)
T13407	59712-59713	.	denotes	.
T13408	59713-59894	sentence	denotes	The promoter was verified by sequencing and digested with XbaI and BglII and subcloned into the pβ-gal BASIC vector (BD Biosciences Clontech, Palo Alto, California, United States).
T13409	59714-59717	DT	denotes	The
T13410	59718-59726	NN	denotes	promoter
T13412	59727-59730	VBD	denotes	was
T13411	59731-59739	VBN	denotes	verified
T13413	59740-59742	IN	denotes	by
T13414	59743-59753	NN	denotes	sequencing
T13415	59754-59757	CC	denotes	and
T13416	59758-59766	VBN	denotes	digested
T13417	59767-59771	IN	denotes	with
T13418	59772-59776	NN	denotes	XbaI
T13419	59777-59780	CC	denotes	and
T13420	59781-59786	NN	denotes	BglII
T13421	59787-59790	CC	denotes	and
T13422	59791-59800	VBN	denotes	subcloned
T13423	59801-59805	IN	denotes	into
T13424	59806-59809	DT	denotes	the
T13426	59810-59812	NN	denotes	pβ
T13428	59812-59813	HYPH	denotes	-
T13427	59813-59816	NN	denotes	gal
T13429	59817-59822	NN	denotes	BASIC
T13425	59823-59829	NN	denotes	vector
T13430	59830-59831	-LRB-	denotes	(
T13432	59831-59833	NNP	denotes	BD
T13433	59834-59845	NNP	denotes	Biosciences
T13431	59846-59854	NNP	denotes	Clontech
T13434	59854-59856	,	denotes	,
T13435	59856-59860	NNP	denotes	Palo
T13436	59861-59865	NNP	denotes	Alto
T13437	59865-59867	,	denotes	,
T13438	59867-59877	NNP	denotes	California
T13439	59877-59879	,	denotes	,
T13440	59879-59885	NNP	denotes	United
T13441	59886-59892	NNP	denotes	States
T13442	59892-59893	-RRB-	denotes	)
T13443	59893-59894	.	denotes	.
T13444	59894-60188	sentence	denotes	The point mutations in the SMAD binding element was generated with the Quik-Change Kit (Stratagene, La Jolla, California, United States) using the forward primer 5′- GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC-3′ and the reverse primer 5′- GCTTTT- CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC-3′.
T13445	59895-59898	DT	denotes	The
T13447	59899-59904	NN	denotes	point
T13446	59905-59914	NNS	denotes	mutations
T13449	59915-59917	IN	denotes	in
T13450	59918-59921	DT	denotes	the
T13452	59922-59926	NN	denotes	SMAD
T13453	59927-59934	NN	denotes	binding
T13451	59935-59942	NN	denotes	element
T13454	59943-59946	VBD	denotes	was
T13448	59947-59956	VBN	denotes	generated
T13455	59957-59961	IN	denotes	with
T13456	59962-59965	DT	denotes	the
T13458	59966-59970	NNP	denotes	Quik
T13460	59970-59971	HYPH	denotes	-
T13459	59971-59977	NNP	denotes	Change
T13457	59978-59981	NNP	denotes	Kit
T13461	59982-59983	-LRB-	denotes	(
T13462	59983-59993	NNP	denotes	Stratagene
T13463	59993-59995	,	denotes	,
T13464	59995-59997	NNP	denotes	La
T13465	59998-60003	NNP	denotes	Jolla
T13466	60003-60005	,	denotes	,
T13467	60005-60015	NNP	denotes	California
T13468	60015-60017	,	denotes	,
T13469	60017-60023	NNP	denotes	United
T13470	60024-60030	NNP	denotes	States
T13471	60030-60031	-RRB-	denotes	)
T13472	60032-60037	VBG	denotes	using
T13473	60038-60041	DT	denotes	the
T13475	60042-60049	JJ	denotes	forward
T13474	60050-60056	NN	denotes	primer
T13476	60057-60058	CD	denotes	5
T13478	60058-60059	SYM	denotes	′
T13479	60059-60060	HYPH	denotes	-
T13477	60061-60106	NN	denotes	GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
T13480	60106-60107	HYPH	denotes	-
T13481	60107-60108	CD	denotes	3
T13482	60108-60109	SYM	denotes	′
T13483	60110-60113	CC	denotes	and
T13484	60114-60117	DT	denotes	the
T13486	60118-60125	JJ	denotes	reverse
T13485	60126-60132	NN	denotes	primer
T13487	60133-60134	CD	denotes	5
T13489	60134-60135	SYM	denotes	′
T13490	60135-60136	HYPH	denotes	-
T13491	60137-60143	NN	denotes	GCTTTT
T13492	60143-60144	HYPH	denotes	-
T13488	60145-60184	NN	denotes	CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
T13493	60184-60185	HYPH	denotes	-
T13494	60185-60186	CD	denotes	3
T13495	60186-60187	SYM	denotes	′
T13496	60187-60188	.	denotes	.
T13497	60188-60421	sentence	denotes	The probes for the Snail in situ hybridization were generated against the 3′ UTR by PCR using the forward primer 5′- ACCTTCTCCCGCATGTCCTTGCTCC-3′ and the reverse primer 5′- CTGCTGAGGCATGGTTACAGCTGG-3′, and genomic DNA as a template.
T13498	60189-60192	DT	denotes	The
T13499	60193-60199	NNS	denotes	probes
T13501	60200-60203	IN	denotes	for
T13502	60204-60207	DT	denotes	the
T13504	60208-60213	NN	denotes	Snail
T13505	60214-60216	FW	denotes	in
T13506	60217-60221	FW	denotes	situ
T13503	60222-60235	NN	denotes	hybridization
T13507	60236-60240	VBD	denotes	were
T13500	60241-60250	VBN	denotes	generated
T13508	60251-60258	IN	denotes	against
T13509	60259-60262	DT	denotes	the
T13511	60263-60264	CD	denotes	3
T13512	60264-60265	SYM	denotes	′
T13510	60266-60269	NN	denotes	UTR
T13513	60270-60272	IN	denotes	by
T13514	60273-60276	NN	denotes	PCR
T13515	60277-60282	VBG	denotes	using
T13516	60283-60286	DT	denotes	the
T13518	60287-60294	JJ	denotes	forward
T13517	60295-60301	NN	denotes	primer
T13519	60302-60303	CD	denotes	5
T13521	60303-60304	SYM	denotes	′
T13522	60304-60305	HYPH	denotes	-
T13520	60306-60331	NN	denotes	ACCTTCTCCCGCATGTCCTTGCTCC
T13523	60331-60332	HYPH	denotes	-
T13524	60332-60333	CD	denotes	3
T13525	60333-60334	SYM	denotes	′
T13526	60335-60338	CC	denotes	and
T13527	60339-60342	DT	denotes	the
T13529	60343-60350	JJ	denotes	reverse
T13528	60351-60357	NN	denotes	primer
T13530	60358-60359	CD	denotes	5
T13532	60359-60360	SYM	denotes	′
T13533	60360-60361	HYPH	denotes	-
T13531	60362-60386	NN	denotes	CTGCTGAGGCATGGTTACAGCTGG
T13534	60386-60387	HYPH	denotes	-
T13535	60387-60388	CD	denotes	3
T13536	60388-60389	SYM	denotes	′
T13537	60389-60391	,	denotes	,
T13538	60391-60394	CC	denotes	and
T13539	60395-60402	JJ	denotes	genomic
T13540	60403-60406	NN	denotes	DNA
T13541	60407-60409	IN	denotes	as
T13542	60410-60411	DT	denotes	a
T13543	60412-60420	NN	denotes	template
T13544	60420-60421	.	denotes	.
T13545	60421-60493	sentence	denotes	The PCR product was gel purified and ligated into pCRII-TOPO TA vector.
T13546	60422-60425	DT	denotes	The
T13548	60426-60429	NN	denotes	PCR
T13547	60430-60437	NN	denotes	product
T13550	60438-60441	VBD	denotes	was
T13551	60442-60445	NN	denotes	gel
T13549	60446-60454	VBN	denotes	purified
T13552	60455-60458	CC	denotes	and
T13553	60459-60466	VBN	denotes	ligated
T13554	60467-60471	IN	denotes	into
T13555	60472-60477	NN	denotes	pCRII
T13557	60477-60478	HYPH	denotes	-
T13556	60478-60482	NN	denotes	TOPO
T13559	60483-60485	NN	denotes	TA
T13558	60486-60492	NN	denotes	vector
T13560	60492-60493	.	denotes	.
T13561	60493-60654	sentence	denotes	The pre-LIM domain of Ajuba was generated essentially as described [9], but was fused to GFP by subcloning from the pEGFP-N1 20 vector (BD Biosciences Clontech)
T13562	60494-60497	DT	denotes	The
T13564	60498-60505	JJ	denotes	pre-LIM
T13563	60506-60512	NN	denotes	domain
T13566	60513-60515	IN	denotes	of
T13567	60516-60521	NN	denotes	Ajuba
T13568	60522-60525	VBD	denotes	was
T13565	60526-60535	VBN	denotes	generated
T13569	60536-60547	RB	denotes	essentially
T13570	60548-60550	IN	denotes	as
T13571	60551-60560	VBN	denotes	described
T13572	60561-60562	-LRB-	denotes	[
T13573	60562-60563	CD	denotes	9
T13574	60563-60564	-RRB-	denotes	]
T13575	60564-60566	,	denotes	,
T13576	60566-60569	CC	denotes	but
T13577	60570-60573	VBD	denotes	was
T13578	60574-60579	VBN	denotes	fused
T13579	60580-60582	IN	denotes	to
T13580	60583-60586	NN	denotes	GFP
T13581	60587-60589	IN	denotes	by
T13582	60590-60600	VBG	denotes	subcloning
T13583	60601-60605	IN	denotes	from
T13584	60606-60609	DT	denotes	the
T13586	60610-60615	NN	denotes	pEGFP
T13588	60615-60616	HYPH	denotes	-
T13587	60616-60618	NN	denotes	N1
T13589	60619-60621	CD	denotes	20
T13585	60622-60628	NN	denotes	vector
T13590	60629-60630	-LRB-	denotes	(
T13592	60630-60632	NNP	denotes	BD
T13593	60633-60644	NNP	denotes	Biosciences
T13591	60645-60653	NNP	denotes	Clontech
T13594	60653-60654	-RRB-	denotes	)
T13649	60656-60658	FW	denotes	In
T13650	60659-60663	FW	denotes	situ
T13651	60664-60677	NN	denotes	hybridization
T13652	60677-60840	sentence	denotes	The pCRII-TOPO TA vector containing a region of the 3′ UTR of Snail was used as a template to generate digoxigenin-labeled sense and antisense riboprobes (Roche).
T13653	60678-60681	DT	denotes	The
T13655	60682-60687	NN	denotes	pCRII
T13657	60687-60688	HYPH	denotes	-
T13656	60688-60692	NN	denotes	TOPO
T13658	60693-60695	NN	denotes	TA
T13654	60696-60702	NN	denotes	vector
T13660	60703-60713	VBG	denotes	containing
T13661	60714-60715	DT	denotes	a
T13662	60716-60722	NN	denotes	region
T13663	60723-60725	IN	denotes	of
T13664	60726-60729	DT	denotes	the
T13666	60730-60731	CD	denotes	3
T13667	60731-60732	SYM	denotes	′
T13665	60733-60736	NN	denotes	UTR
T13668	60737-60739	IN	denotes	of
T13669	60740-60745	NN	denotes	Snail
T13670	60746-60749	VBD	denotes	was
T13659	60750-60754	VBN	denotes	used
T13671	60755-60757	IN	denotes	as
T13672	60758-60759	DT	denotes	a
T13673	60760-60768	NN	denotes	template
T13674	60769-60771	TO	denotes	to
T13675	60772-60780	VB	denotes	generate
T13676	60781-60792	NN	denotes	digoxigenin
T13678	60792-60793	HYPH	denotes	-
T13677	60793-60800	VBN	denotes	labeled
T13680	60801-60806	NN	denotes	sense
T13681	60807-60810	CC	denotes	and
T13682	60811-60820	NN	denotes	antisense
T13679	60821-60831	NNS	denotes	riboprobes
T13683	60832-60833	-LRB-	denotes	(
T13684	60833-60838	NNP	denotes	Roche
T13685	60838-60839	-RRB-	denotes	)
T13686	60839-60840	.	denotes	.
T13687	60840-60906	sentence	denotes	The respective probes were obtained by XhoI and BamH1 digestions.
T13688	60841-60844	DT	denotes	The
T13690	60845-60855	JJ	denotes	respective
T13689	60856-60862	NNS	denotes	probes
T13692	60863-60867	VBD	denotes	were
T13691	60868-60876	VBN	denotes	obtained
T13693	60877-60879	IN	denotes	by
T13694	60880-60884	NN	denotes	XhoI
T13696	60885-60888	CC	denotes	and
T13697	60889-60894	NN	denotes	BamH1
T13695	60895-60905	NNS	denotes	digestions
T13698	60905-60906	.	denotes	.
T13699	60906-60992	sentence	denotes	In situ hybridizations were performed on 10-μm thick sections of E17.5 mouse embryos.
T13700	60907-60909	FW	denotes	In
T13701	60910-60914	FW	denotes	situ
T13702	60915-60929	NNS	denotes	hybridizations
T13704	60930-60934	VBD	denotes	were
T13703	60935-60944	VBN	denotes	performed
T13705	60945-60947	IN	denotes	on
T13706	60948-60950	CD	denotes	10
T13708	60950-60951	HYPH	denotes	-
T13707	60951-60953	NN	denotes	μm
T13709	60954-60959	JJ	denotes	thick
T13710	60960-60968	NNS	denotes	sections
T13711	60969-60971	IN	denotes	of
T13712	60972-60977	NN	denotes	E17.5
T13714	60978-60983	NN	denotes	mouse
T13713	60984-60991	NNS	denotes	embryos
T13715	60991-60992	.	denotes	.
T13716	60992-61275	sentence	denotes	The sections were fixed with 4% PFA for 10 min at room temperature, prehybridized at room temperature for 4.5 h, hybridized with the probe (2 μg/ml) at 55 °C for 12–14 h, blocked with 10% NGS, and treated with anti-dig Fab-AP antibody (Roche #1093274) at a 1:2,500 dilution for 3 h.
T13717	60993-60996	DT	denotes	The
T13718	60997-61005	NNS	denotes	sections
T13720	61006-61010	VBD	denotes	were
T13719	61011-61016	VBN	denotes	fixed
T13721	61017-61021	IN	denotes	with
T13722	61022-61023	CD	denotes	4
T13723	61023-61024	NN	denotes	%
T13724	61025-61028	NN	denotes	PFA
T13725	61029-61032	IN	denotes	for
T13726	61033-61035	CD	denotes	10
T13727	61036-61039	NN	denotes	min
T13728	61040-61042	IN	denotes	at
T13729	61043-61047	NN	denotes	room
T13730	61048-61059	NN	denotes	temperature
T13731	61059-61061	,	denotes	,
T13732	61061-61074	VBN	denotes	prehybridized
T13733	61075-61077	IN	denotes	at
T13734	61078-61082	NN	denotes	room
T13735	61083-61094	NN	denotes	temperature
T13736	61095-61098	IN	denotes	for
T13737	61099-61102	CD	denotes	4.5
T13738	61103-61104	NN	denotes	h
T13739	61104-61106	,	denotes	,
T13740	61106-61116	VBN	denotes	hybridized
T13741	61117-61121	IN	denotes	with
T13742	61122-61125	DT	denotes	the
T13743	61126-61131	NN	denotes	probe
T13744	61132-61133	-LRB-	denotes	(
T13746	61133-61134	CD	denotes	2
T13745	61135-61137	NN	denotes	μg
T13747	61137-61138	SYM	denotes	/
T13748	61138-61140	NN	denotes	ml
T13749	61140-61141	-RRB-	denotes	)
T13750	61142-61144	IN	denotes	at
T13751	61145-61147	CD	denotes	55
T13752	61148-61150	NN	denotes	°C
T13753	61151-61154	IN	denotes	for
T13754	61155-61157	CD	denotes	12
T13756	61157-61158	SYM	denotes	–
T13755	61158-61160	CD	denotes	14
T13757	61161-61162	NN	denotes	h
T13758	61162-61164	,	denotes	,
T13759	61164-61171	VBN	denotes	blocked
T13760	61172-61176	IN	denotes	with
T13761	61177-61179	CD	denotes	10
T13762	61179-61180	NN	denotes	%
T13763	61181-61184	NN	denotes	NGS
T13764	61184-61186	,	denotes	,
T13765	61186-61189	CC	denotes	and
T13766	61190-61197	VBN	denotes	treated
T13767	61198-61202	IN	denotes	with
T13768	61203-61211	JJ	denotes	anti-dig
T13770	61212-61215	NN	denotes	Fab
T13772	61215-61216	HYPH	denotes	-
T13771	61216-61218	NN	denotes	AP
T13769	61219-61227	NN	denotes	antibody
T13773	61228-61229	-LRB-	denotes	(
T13774	61229-61234	NNP	denotes	Roche
T13775	61235-61236	SYM	denotes	#
T13776	61236-61243	CD	denotes	1093274
T13777	61243-61244	-RRB-	denotes	)
T13778	61245-61247	IN	denotes	at
T13779	61248-61249	DT	denotes	a
T13781	61250-61251	CD	denotes	1
T13783	61251-61252	SYM	denotes	:
T13782	61252-61257	CD	denotes	2,500
T13780	61258-61266	NN	denotes	dilution
T13784	61267-61270	IN	denotes	for
T13785	61271-61272	CD	denotes	3
T13786	61273-61274	NN	denotes	h
T13787	61274-61275	.	denotes	.
T13788	61275-61357	sentence	denotes	The sections were incubated with NBT and BCIP until adequate signal was detected.
T13789	61276-61279	DT	denotes	The
T13790	61280-61288	NNS	denotes	sections
T13792	61289-61293	VBD	denotes	were
T13791	61294-61303	VBN	denotes	incubated
T13793	61304-61308	IN	denotes	with
T13794	61309-61312	NN	denotes	NBT
T13795	61313-61316	CC	denotes	and
T13796	61317-61321	NN	denotes	BCIP
T13797	61322-61327	IN	denotes	until
T13799	61328-61336	JJ	denotes	adequate
T13800	61337-61343	NN	denotes	signal
T13801	61344-61347	VBD	denotes	was
T13798	61348-61356	VBN	denotes	detected
T13802	61356-61357	.	denotes	.
T13828	61359-61377	NN	denotes	Immunofluorescence
T13829	61378-61381	CC	denotes	and
T13830	61382-61402	NN	denotes	immunohistochemistry
T13831	61402-61500	sentence	denotes	Tissue samples for immunofluorescence were frozen in OCT and sectioned 10 μm thick on a cryostat.
T13832	61403-61409	NN	denotes	Tissue
T13833	61410-61417	NNS	denotes	samples
T13835	61418-61421	IN	denotes	for
T13836	61422-61440	NN	denotes	immunofluorescence
T13837	61441-61445	VBD	denotes	were
T13834	61446-61452	VBN	denotes	frozen
T13838	61453-61455	IN	denotes	in
T13839	61456-61459	NN	denotes	OCT
T13840	61460-61463	CC	denotes	and
T13841	61464-61473	VBN	denotes	sectioned
T13842	61474-61476	CD	denotes	10
T13843	61477-61479	NN	denotes	μm
T13844	61480-61485	JJ	denotes	thick
T13845	61486-61488	IN	denotes	on
T13846	61489-61490	DT	denotes	a
T13847	61491-61499	NN	denotes	cryostat
T13848	61499-61500	.	denotes	.
T13849	61500-61613	sentence	denotes	Sections were fixed in 4% paraformaldehyde for 10 min at room temperature, blocked, and stained with antibodies.
T13850	61501-61509	NNS	denotes	Sections
T13852	61510-61514	VBD	denotes	were
T13851	61515-61520	VBN	denotes	fixed
T13853	61521-61523	IN	denotes	in
T13854	61524-61525	CD	denotes	4
T13855	61525-61526	NN	denotes	%
T13856	61527-61543	NN	denotes	paraformaldehyde
T13857	61544-61547	IN	denotes	for
T13858	61548-61550	CD	denotes	10
T13859	61551-61554	NN	denotes	min
T13860	61555-61557	IN	denotes	at
T13861	61558-61562	NN	denotes	room
T13862	61563-61574	NN	denotes	temperature
T13863	61574-61576	,	denotes	,
T13864	61576-61583	VBN	denotes	blocked
T13865	61583-61585	,	denotes	,
T13866	61585-61588	CC	denotes	and
T13867	61589-61596	VBN	denotes	stained
T13868	61597-61601	IN	denotes	with
T13869	61602-61612	NNS	denotes	antibodies
T13870	61612-61613	.	denotes	.
T13871	61613-61726	sentence	denotes	Tissue samples for immunohistochemistry were fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin.
T13872	61614-61620	NN	denotes	Tissue
T13873	61621-61628	NNS	denotes	samples
T13875	61629-61632	IN	denotes	for
T13876	61633-61653	NN	denotes	immunohistochemistry
T13877	61654-61658	VBD	denotes	were
T13874	61659-61664	VBN	denotes	fixed
T13878	61665-61667	IN	denotes	in
T13879	61668-61669	CD	denotes	4
T13880	61669-61670	NN	denotes	%
T13881	61671-61687	NN	denotes	paraformaldehyde
T13882	61687-61689	,	denotes	,
T13883	61689-61699	VBN	denotes	dehydrated
T13884	61699-61701	,	denotes	,
T13885	61701-61704	CC	denotes	and
T13886	61705-61713	VBN	denotes	embedded
T13887	61714-61716	IN	denotes	in
T13888	61717-61725	NN	denotes	paraffin
T13889	61725-61726	.	denotes	.
T13890	61726-61826	sentence	denotes	Samples were sectioned on a microtome (10 μm thick) and rehydrated prior to staining with antibody.
T13891	61727-61734	NNS	denotes	Samples
T13893	61735-61739	VBD	denotes	were
T13892	61740-61749	VBN	denotes	sectioned
T13894	61750-61752	IN	denotes	on
T13895	61753-61754	DT	denotes	a
T13896	61755-61764	NN	denotes	microtome
T13897	61765-61766	-LRB-	denotes	(
T13899	61766-61768	CD	denotes	10
T13900	61769-61771	NN	denotes	μm
T13898	61772-61777	JJ	denotes	thick
T13901	61777-61778	-RRB-	denotes	)
T13902	61779-61782	CC	denotes	and
T13903	61783-61793	VBD	denotes	rehydrated
T13904	61794-61799	IN	denotes	prior
T13905	61800-61802	IN	denotes	to
T13906	61803-61811	VBG	denotes	staining
T13907	61812-61816	IN	denotes	with
T13908	61817-61825	NN	denotes	antibody
T13909	61825-61826	.	denotes	.
T13910	61826-62012	sentence	denotes	Samples stained with Snail, pMAPK, pSmad2, and cyclin D were antigen unmasked with 10 mM sodium citrate (pH 6) in an Antigen Retriever 2100 (Pickcell Laboratories, Leiden, Netherlands).
T13911	61827-61834	NNS	denotes	Samples
T13913	61835-61842	VBN	denotes	stained
T13914	61843-61847	IN	denotes	with
T13915	61848-61853	NN	denotes	Snail
T13916	61853-61855	,	denotes	,
T13917	61855-61860	NN	denotes	pMAPK
T13918	61860-61862	,	denotes	,
T13919	61862-61868	NN	denotes	pSmad2
T13920	61868-61870	,	denotes	,
T13921	61870-61873	CC	denotes	and
T13922	61874-61880	NN	denotes	cyclin
T13923	61881-61882	NN	denotes	D
T13924	61883-61887	VBD	denotes	were
T13925	61888-61895	NN	denotes	antigen
T13912	61896-61904	VBN	denotes	unmasked
T13926	61905-61909	IN	denotes	with
T13927	61910-61912	CD	denotes	10
T13928	61913-61915	NN	denotes	mM
T13930	61916-61922	NN	denotes	sodium
T13929	61923-61930	NN	denotes	citrate
T13931	61931-61932	-LRB-	denotes	(
T13932	61932-61934	NN	denotes	pH
T13933	61935-61936	CD	denotes	6
T13934	61936-61937	-RRB-	denotes	)
T13935	61938-61940	IN	denotes	in
T13936	61941-61943	DT	denotes	an
T13938	61944-61951	NNP	denotes	Antigen
T13937	61952-61961	NNP	denotes	Retriever
T13939	61962-61966	CD	denotes	2100
T13940	61967-61968	-LRB-	denotes	(
T13942	61968-61976	NNP	denotes	Pickcell
T13941	61977-61989	NNP	denotes	Laboratories
T13943	61989-61991	,	denotes	,
T13944	61991-61997	NNP	denotes	Leiden
T13945	61997-61999	,	denotes	,
T13946	61999-62010	NNP	denotes	Netherlands
T13947	62010-62011	-RRB-	denotes	)
T13948	62011-62012	.	denotes	.
T13949	62012-62121	sentence	denotes	The DAB substrate kit (Vector Labs) was used according to manufacturer's instructions to develop the signal.
T13950	62013-62016	DT	denotes	The
T13952	62017-62020	NN	denotes	DAB
T13953	62021-62030	NN	denotes	substrate
T13951	62031-62034	NN	denotes	kit
T13955	62035-62036	-LRB-	denotes	(
T13957	62036-62042	NNP	denotes	Vector
T13956	62043-62047	NNPS	denotes	Labs
T13958	62047-62048	-RRB-	denotes	)
T13959	62049-62052	VBD	denotes	was
T13954	62053-62057	VBN	denotes	used
T13960	62058-62067	VBG	denotes	according
T13961	62068-62070	IN	denotes	to
T13962	62071-62083	NN	denotes	manufacturer
T13964	62083-62085	POS	denotes	's
T13963	62086-62098	NNS	denotes	instructions
T13965	62099-62101	TO	denotes	to
T13966	62102-62109	VB	denotes	develop
T13967	62110-62113	DT	denotes	the
T13968	62114-62120	NN	denotes	signal
T13969	62120-62121	.	denotes	.
T14008	62123-62125	NN	denotes	RT
T14010	62125-62126	HYPH	denotes	-
T14009	62126-62129	NN	denotes	PCR
T14011	62129-62248	sentence	denotes	RNA was extracted from keratinocytes or skin tissue with Trizol (Invitrogen) according to the manufacturer's protocol.
T14012	62130-62133	NN	denotes	RNA
T14014	62134-62137	VBD	denotes	was
T14013	62138-62147	VBN	denotes	extracted
T14015	62148-62152	IN	denotes	from
T14016	62153-62166	NNS	denotes	keratinocytes
T14017	62167-62169	CC	denotes	or
T14018	62170-62174	NN	denotes	skin
T14019	62175-62181	NN	denotes	tissue
T14020	62182-62186	IN	denotes	with
T14021	62187-62193	NN	denotes	Trizol
T14022	62194-62195	-LRB-	denotes	(
T14023	62195-62205	NNP	denotes	Invitrogen
T14024	62205-62206	-RRB-	denotes	)
T14025	62207-62216	VBG	denotes	according
T14026	62217-62219	IN	denotes	to
T14027	62220-62223	DT	denotes	the
T14028	62224-62236	NN	denotes	manufacturer
T14030	62236-62238	POS	denotes	's
T14029	62239-62247	NN	denotes	protocol
T14031	62247-62248	.	denotes	.
T14032	62248-62331	sentence	denotes	cDNA was generated using oligo-dT primers and the Superscript II kit (Invitrogen).
T14033	62249-62253	NN	denotes	cDNA
T14035	62254-62257	VBD	denotes	was
T14034	62258-62267	VBN	denotes	generated
T14036	62268-62273	VBG	denotes	using
T14037	62274-62279	NN	denotes	oligo
T14039	62279-62280	HYPH	denotes	-
T14038	62280-62282	NN	denotes	dT
T14040	62283-62290	NNS	denotes	primers
T14041	62291-62294	CC	denotes	and
T14042	62295-62298	DT	denotes	the
T14044	62299-62310	NNP	denotes	Superscript
T14045	62311-62313	CD	denotes	II
T14043	62314-62317	NN	denotes	kit
T14046	62318-62319	-LRB-	denotes	(
T14047	62319-62329	NNP	denotes	Invitrogen
T14048	62329-62330	-RRB-	denotes	)
T14049	62330-62331	.	denotes	.
T14050	62331-62544	sentence	denotes	The primers used for PCR were Snail forward: 5′- CAGCTGGCCAGGCTCTCGGT-3′; Snail reverse: 5′- GCGAGGGCCTCCGGAGCA-3′; GAPDH forward 5′- CGTAGACAAAATGGTGAAGGTCGG-3′; and GAPDH reverse: 5′- AAGCAGTTGGTGGTGCAGGATG-3′.
T14051	62332-62335	DT	denotes	The
T14052	62336-62343	NNS	denotes	primers
T14054	62344-62348	VBN	denotes	used
T14055	62349-62352	IN	denotes	for
T14056	62353-62356	NN	denotes	PCR
T14053	62357-62361	VBD	denotes	were
T14057	62362-62367	NN	denotes	Snail
T14058	62368-62375	NN	denotes	forward
T14059	62375-62377	:	denotes	:
T14060	62377-62378	CD	denotes	5
T14062	62378-62379	SYM	denotes	′
T14063	62379-62380	HYPH	denotes	-
T14061	62381-62401	NN	denotes	CAGCTGGCCAGGCTCTCGGT
T14064	62401-62402	HYPH	denotes	-
T14065	62402-62403	CD	denotes	3
T14066	62403-62404	SYM	denotes	′
T14067	62404-62405	:	denotes	;
T14068	62406-62411	NN	denotes	Snail
T14069	62412-62419	NN	denotes	reverse
T14070	62419-62421	:	denotes	:
T14071	62421-62422	CD	denotes	5
T14073	62422-62423	SYM	denotes	′
T14074	62423-62424	HYPH	denotes	-
T14072	62425-62443	NN	denotes	GCGAGGGCCTCCGGAGCA
T14075	62443-62444	HYPH	denotes	-
T14076	62444-62445	CD	denotes	3
T14077	62445-62446	SYM	denotes	′
T14078	62446-62447	:	denotes	;
T14079	62448-62453	NN	denotes	GAPDH
T14080	62454-62461	NN	denotes	forward
T14081	62462-62463	CD	denotes	5
T14083	62463-62464	SYM	denotes	′
T14084	62464-62465	HYPH	denotes	-
T14082	62466-62490	NN	denotes	CGTAGACAAAATGGTGAAGGTCGG
T14085	62490-62491	HYPH	denotes	-
T14086	62491-62492	CD	denotes	3
T14087	62492-62493	SYM	denotes	′
T14088	62493-62494	:	denotes	;
T14089	62495-62498	CC	denotes	and
T14090	62499-62504	NN	denotes	GAPDH
T14091	62505-62512	NN	denotes	reverse
T14092	62512-62514	:	denotes	:
T14093	62514-62515	CD	denotes	5
T14095	62515-62516	SYM	denotes	′
T14096	62516-62517	HYPH	denotes	-
T14094	62518-62540	NN	denotes	AAGCAGTTGGTGGTGCAGGATG
T14097	62540-62541	HYPH	denotes	-
T14098	62541-62542	CD	denotes	3
T14099	62542-62543	SYM	denotes	′
T14100	62543-62544	.	denotes	.
R1000	T1864	T1862	pobj	proximity,by
R1001	T1865	T1864	amod	close,proximity
R1002	T1757	T1756	pobj	proteins,of
R1003	T1866	T1864	prep	of,proximity
R1004	T1867	T1868	det	the,site
R1005	T1758	T1731	punct	),protein
R1006	T1759	T1731	punct	", ",protein
R1007	T1868	T1866	pobj	site,of
R1008	T1869	T1868	nmod	LEF,site
R1009	T1870	T1869	punct	-,LEF
R1010	T1760	T1761	dep	which,appears
R1011	T1871	T1869	nummod	1,LEF
R1012	T1872	T1869	punct	/,LEF
R1013	T1761	T1731	relcl	appears,protein
R1014	T1873	T1874	compound	β,catenin
R1015	T1762	T1763	aux	to,function
R1016	T1874	T1869	appos	catenin,LEF
R1017	T1875	T1874	punct	-,catenin
R1018	T1876	T1868	compound	binding,site
R1019	T1763	T1761	xcomp	function,appears
R1020	T1877	T1864	prep	to,proximity
R1021	T1878	T1879	det	a,site
R1022	T1879	T1877	pobj	site,to
R1023	T1764	T1763	advmod	dually,function
R1024	T1880	T1879	acl	known,site
R1025	T1881	T1882	aux	to,bind
R1026	T1765	T1763	prep	in,function
R1027	T1882	T1880	xcomp	bind,known
R1028	T1883	T1884	det	the,family
R1029	T1884	T1882	dobj	family,bind
R1030	T1766	T1765	preconj	both,in
R1031	T1767	T1765	conj	adhesion,in
R1032	T1768	T1765	cc	and,in
R1033	T1769	T1765	conj	in,in
R1034	T1885	T1886	compound	Snail,Slug
R1035	T1886	T1884	compound	Slug,family
R1036	T1770	T1769	pobj	activation,in
R1037	T1887	T1886	punct	/,Slug
R1038	T1888	T1884	prep	of,family
R1039	T1889	T1890	nmod	zinc,finger
R1040	T1771	T1770	prep	of,activation
R1041	T1890	T1891	nmod	finger,proteins
R1042	T1891	T1888	pobj	proteins,of
R1043	T1772	T1773	det	the,pathway
R1044	T1892	T1893	amod	transcriptional,repressor
R1045	T1893	T1891	compound	repressor,proteins
R1046	T1894	T1895	punct	[,15
R1047	T1773	T1771	pobj	pathway,of
R1048	T1895	T1846	parataxis	15,intrigued
R1049	T1896	T1895	nummod	12,15
R1050	T1897	T1895	punct	",",15
R1051	T1898	T1895	nummod	13,15
R1052	T1899	T1895	punct	",",15
R1053	T1900	T1895	nummod	14,15
R1054	T1774	T1773	nmod	Ras,pathway
R1055	T1901	T1895	punct	",",15
R1056	T1902	T1895	punct	],15
R1057	T1903	T1846	punct	.,intrigued
R1058	T1775	T1774	punct	-,Ras
R1059	T1905	T1906	preconj	Both,activity
R1060	T1776	T1777	npadvmod	mitogen,activated
R1061	T1906	T1907	nsubj	activity,play
R1062	T1908	T1906	prep	of,activity
R1063	T1777	T1779	amod	activated,kinase
R1064	T1909	T1908	pobj	Snail,of
R1065	T1910	T1906	cc	and,activity
R1066	T1911	T1912	compound	down,regulation
R1067	T1778	T1777	punct	-,activated
R1068	T1912	T1906	conj	regulation,activity
R1069	T1913	T1912	punct	-,regulation
R1070	T1914	T1912	prep	of,regulation
R1071	T1779	T1774	appos	kinase,Ras
R1072	T1915	T1916	compound	E,cadherin
R1073	T1916	T1914	pobj	cadherin,of
R1074	T1991	T1988	acl	tune,ability
R1075	T1917	T1916	punct	-,cadherin
R1076	T1918	T1919	amod	pivotal,roles
R1077	T1919	T1907	dobj	roles,play
R1078	T1920	T1907	prep	in,play
R1079	T1992	T1991	advmod	fine,tune
R1080	T1921	T1922	amod	epithelial,transitions
R1081	T1922	T1920	pobj	transitions,in
R1082	T1923	T1921	prep	to,epithelial
R1083	T1993	T1991	punct	-,tune
R1084	T1924	T1923	amod	mesenchymal,to
R1085	T1925	T1922	punct	(,transitions
R1086	T1994	T1995	compound	cadherin,expression
R1087	T1926	T1922	appos	EMTs,transitions
R1088	T1927	T1922	punct	),transitions
R1089	T1928	T1922	punct	", ",transitions
R1090	T1995	T1991	dobj	expression,tune
R1091	T1929	T1922	acl	typified,transitions
R1092	T1930	T1929	agent	by,typified
R1093	T1931	T1932	det	the,transformation
R1094	T1996	T1991	prep	at,tune
R1095	T1932	T1930	pobj	transformation,by
R1096	T1933	T1932	prep	of,transformation
R1097	T1934	T1935	amod	polarized,cells
R1098	T1997	T1998	det	the,level
R1099	T1935	T1933	pobj	cells,of
R1100	T1936	T1935	punct	", ",cells
R1101	T1937	T1935	amod	adhering,cells
R1102	T1938	T1935	amod	epithelial,cells
R1103	T1939	T1932	prep	into,transformation
R1104	T1998	T1996	pobj	level,at
R1105	T1940	T1941	amod	motile,cells
R1106	T1941	T1939	pobj	cells,into
R1107	T1999	T1998	amod	transcriptional,level
R1108	T1942	T1941	amod	mesenchymal,cells
R1109	T1943	T1944	punct	[,17
R1110	T1944	T1907	parataxis	17,play
R1111	T2000	T1985	punct	", ",seems
R1112	T1945	T1944	nummod	16,17
R1113	T1946	T1944	punct	",",17
R1114	T1947	T1944	punct	],17
R1115	T2001	T1985	nsubj	Snail,seems
R1116	T1948	T1907	punct	.,play
R1117	T1950	T1951	compound	Bud,formation
R1118	T2002	T2003	aux	to,have
R1119	T1951	T1952	nsubj	formation,differs
R1120	T2003	T1985	xcomp	have,seems
R1121	T1953	T1952	prep	from,differs
R1122	T1954	T1955	det	an,EMT
R1123	T2004	T2005	det	an,effect
R1124	T1955	T1953	pobj	EMT,from
R1125	T1956	T1957	mark	in,needs
R1126	T2005	T2003	dobj	effect,have
R1127	T2006	T2005	amod	additive,effect
R1128	T1957	T1952	advcl	needs,differs
R1129	T1958	T1957	mark	that,needs
R1130	T1959	T1960	compound	E,cadherin
R1131	T2007	T2003	prep	with,have
R1132	T1960	T1962	compound	cadherin,activity
R1133	T1961	T1960	punct	-,cadherin
R1134	T1962	T1957	nsubj	activity,needs
R1135	T2008	T2007	pobj	LEF,with
R1136	T1963	T1964	aux	to,regulated
R1137	T1964	T1957	xcomp	regulated,needs
R1138	T1965	T1964	aux	be,regulated
R1139	T2009	T2008	punct	-,LEF
R1140	T1966	T1964	advmod	down,regulated
R1141	T1967	T1964	punct	-,regulated
R1142	T2010	T2008	nummod	1,LEF
R1143	T1968	T1964	cc	but,regulated
R1144	T1969	T1968	neg	not,but
R1145	T2011	T2008	punct	/,LEF
R1146	T1970	T1964	conj	prevented,regulated
R1147	T1971	T1964	punct	", ",regulated
R1148	T1972	T1973	mark	so,remodeled
R1149	T2012	T2013	compound	β,catenin
R1150	T1973	T1964	advcl	remodeled,regulated
R1151	T1974	T1973	mark	that,remodeled
R1152	T2013	T2008	appos	catenin,LEF
R1153	T1975	T1976	amod	adhesive,junctions
R1154	T1976	T1973	nsubjpass	junctions,remodeled
R1155	T1977	T1973	auxpass	are,remodeled
R1156	T1978	T1979	advmod	rather,than
R1157	T1979	T1973	cc	than,remodeled
R1158	T1980	T1981	advmod	quantitatively,impaired
R1159	T1981	T1973	conj	impaired,remodeled
R1160	T2014	T2013	punct	-,catenin
R1161	T1982	T1952	punct	.,differs
R1162	T2015	T2003	prep	in,have
R1163	T1984	T1985	advcl	Supportive,seems
R1164	T1986	T1984	prep	of,Supportive
R1165	T2016	T2017	advmod	negatively,modulating
R1166	T1987	T1988	det	an,ability
R1167	T1988	T1986	pobj	ability,of
R1168	T1989	T1988	amod	underlying,ability
R1169	T2017	T2015	pcomp	modulating,in
R1170	T1990	T1991	aux	to,tune
R1171	T2018	T2019	compound	E,cadherin
R1172	T2019	T2021	compound	cadherin,promoter
R1173	T2020	T2019	punct	-,cadherin
R1174	T2021	T2022	compound	promoter,activity
R1175	T2022	T2017	dobj	activity,modulating
R1176	T2023	T2024	punct	[,4
R1177	T2097	T2096	acl	associated,features
R1178	T2098	T2097	prep	with,associated
R1179	T2024	T1985	parataxis	4,seems
R1180	T2099	T2100	compound	hair,bud
R1181	T2100	T2101	compound	bud,formation
R1182	T2025	T2024	punct	],4
R1183	T2101	T2098	pobj	formation,with
R1184	T2102	T2096	punct	", ",features
R1185	T2103	T2096	prep	including,features
R1186	T2026	T1985	punct	.,seems
R1187	T2104	T2105	compound	down,regulation
R1188	T2105	T2103	pobj	regulation,including
R1189	T2028	T2029	prep	In,discovered
R1190	T2106	T2105	punct	-,regulation
R1191	T2107	T2105	prep	of,regulation
R1192	T2108	T2109	compound	E,cadherin
R1193	T2109	T2107	pobj	cadherin,of
R1194	T2030	T2031	det	the,study
R1195	T2110	T2109	punct	-,cadherin
R1196	T2111	T2105	punct	", ",regulation
R1197	T2112	T2113	amod	increased,proliferation
R1198	T2113	T2105	conj	proliferation,regulation
R1199	T2031	T2028	pobj	study,In
R1200	T2114	T2113	punct	", ",proliferation
R1201	T2115	T2113	cc	and,proliferation
R1202	T2032	T2031	amod	present,study
R1203	T2116	T2117	amod	repressed,differentiation
R1204	T2117	T2113	conj	differentiation,proliferation
R1205	T2118	T2117	amod	terminal,differentiation
R1206	T2119	T2084	punct	.,appears
R1207	T2033	T2029	punct	", ",discovered
R1208	T2121	T2122	mark	Although,reflected
R1209	T2034	T2029	nsubj	we,discovered
R1210	T2122	T2133	advcl	reflected,appear
R1211	T2123	T2124	det	the,spike
R1212	T2035	T2036	mark	that,expressed
R1213	T2124	T2122	nsubjpass	spike,reflected
R1214	T2125	T2124	amod	temporal,spike
R1215	T2036	T2029	ccomp	expressed,discovered
R1216	T2126	T2124	prep	of,spike
R1217	T2127	T2126	pobj	Snail,of
R1218	T2037	T2036	nsubjpass	Snail,expressed
R1219	T2128	T2124	prep	in,spike
R1220	T2129	T2130	det	the,bud
R1221	T2130	T2128	pobj	bud,in
R1222	T2038	T2036	auxpass	is,expressed
R1223	T2131	T2130	compound	hair,bud
R1224	T2132	T2122	auxpass	is,reflected
R1225	T2039	T2036	advmod	briefly,expressed
R1226	T2134	T2122	prep	at,reflected
R1227	T2135	T2136	det	the,level
R1228	T2136	T2134	pobj	level,at
R1229	T2040	T2036	prep	at,expressed
R1230	T2137	T2136	compound	mRNA,level
R1231	T2138	T2122	cc	and,reflected
R1232	T2139	T2122	conj	seems,reflected
R1233	T2041	T2042	det	an,stage
R1234	T2140	T2141	aux	to,follow
R1235	T2141	T2139	xcomp	follow,seems
R1236	T2142	T2143	compound	Wnt,signaling
R1237	T2042	T2040	pobj	stage,at
R1238	T2143	T2141	dobj	signaling,follow
R1239	T2144	T2143	cc	and,signaling
R1240	T2043	T2042	amod	early,stage
R1241	T2145	T2146	compound	BMP,inhibition
R1242	T2146	T2143	conj	inhibition,signaling
R1243	T2147	T2133	punct	", ",appear
R1244	T2044	T2042	prep	of,stage
R1245	T2148	T2149	nmod	LEF,activation
R1246	T2149	T2133	nsubj	activation,appear
R1247	T2045	T2046	compound	hair,bud
R1248	T2150	T2148	punct	-,LEF
R1249	T2151	T2148	nummod	1,LEF
R1250	T2152	T2148	punct	/,LEF
R1251	T2046	T2047	compound	bud,formation
R1252	T2153	T2154	compound	β,catenin
R1253	T2154	T2148	appos	catenin,LEF
R1254	T2155	T2154	punct	-,catenin
R1255	T2047	T2044	pobj	formation,of
R1256	T2156	T2133	aux	does,appear
R1257	T2157	T2133	neg	not,appear
R1258	T2158	T2159	aux	to,induce
R1259	T2159	T2133	xcomp	induce,appear
R1260	T2160	T2161	compound	Snail,expression
R1261	T2161	T2159	dobj	expression,induce
R1262	T2162	T2161	compound	gene,expression
R1263	T2048	T2042	punct	", ",stage
R1264	T2163	T2159	prep	in,induce
R1265	T2164	T2165	amod	embryonic,keratinocytes
R1266	T2165	T2163	pobj	keratinocytes,in
R1267	T2166	T2165	compound	skin,keratinocytes
R1268	T2049	T2050	advmod	when,take
R1269	T2167	T2133	punct	.,appear
R1270	T2050	T2042	relcl	take,stage
R1271	T2169	T2170	prep	In,provide
R1272	T2171	T2169	pobj	contrast,In
R1273	T2051	T2052	compound	E,cadherin
R1274	T2172	T2170	punct	", ",provide
R1275	T2173	T2170	nsubj	we,provide
R1276	T2174	T2175	advmod	in,vitro
R1277	T2052	T2050	nsubj	cadherin,take
R1278	T2175	T2176	amod	vitro,evidence
R1279	T2176	T2170	dobj	evidence,provide
R1280	T2053	T2052	punct	-,cadherin
R1281	T2177	T2175	punct	", ",vitro
R1282	T2178	T2175	conj	transgenic,vitro
R1283	T2179	T2180	punct	(,Tg
R1284	T2054	T2055	amod	down,regulation
R1285	T2180	T2178	parataxis	Tg,transgenic
R1286	T2181	T2180	punct	),Tg
R1287	T2182	T2178	punct	", ",transgenic
R1288	T2055	T2052	appos	regulation,cadherin
R1289	T2183	T2178	cc	and,transgenic
R1290	T2184	T2185	npadvmod	gene,targeting
R1291	T2056	T2055	punct	-,regulation
R1292	T2185	T2178	conj	targeting,transgenic
R1293	T2186	T2187	aux	to,show
R1294	T2057	T2055	cc	and,regulation
R1295	T2187	T2170	advcl	show,provide
R1296	T2188	T2189	mark	that,are
R1297	T2058	T2055	conj	activation,regulation
R1298	T2059	T2058	prep	of,activation
R1299	T2189	T2187	ccomp	are,show
R1300	T2190	T2191	nmod	TGF,β2
R1301	T2060	T2059	pobj	proliferation,of
R1302	T2191	T2193	nmod	β2,signaling
R1303	T2192	T2191	punct	-,β2
R1304	T2193	T2189	nsubj	signaling,are
R1305	T2061	T2050	dobj	place,take
R1306	T2194	T2191	cc	and,β2
R1307	T2195	T2196	amod	small,phenotype
R1308	T2196	T2197	npadvmod	phenotype,related
R1309	T2197	T2204	amod	related,protein
R1310	T2198	T2196	punct	–,phenotype
R1311	T2199	T2196	cc	and,phenotype
R1312	T2062	T2029	punct	.,discovered
R1313	T2200	T2196	conj	mothers,phenotype
R1314	T2201	T2200	prep	against,mothers
R1315	T2202	T2201	pobj	decapentaplegic,against
R1316	T2064	T2065	advmod	Thereafter,disappears
R1317	T2066	T2065	punct	", ",disappears
R1318	T2203	T2197	punct	–,related
R1319	T2204	T2191	conj	protein,β2
R1320	T2067	T2065	nsubj	Snail,disappears
R1321	T2205	T2204	nummod	2,protein
R1322	T2206	T2204	punct	(,protein
R1323	T2207	T2204	appos	SMAD2,protein
R1324	T2068	T2065	cc	and,disappears
R1325	T2208	T2193	punct	),signaling
R1326	T2209	T2210	amod	upstream,inducers
R1327	T2069	T2065	conj	remains,disappears
R1328	T2210	T2189	attr	inducers,are
R1329	T2211	T2210	prep	of,inducers
R1330	T2212	T2213	compound	Snail,expression
R1331	T2070	T2069	acomp	absent,remains
R1332	T2213	T2211	pobj	expression,of
R1333	T2214	T2213	compound	gene,expression
R1334	T2071	T2069	prep	during,remains
R1335	T2215	T2189	prep	in,are
R1336	T2216	T2217	compound	skin,epithelium
R1337	T2072	T2073	amod	subsequent,growth
R1338	T2217	T2215	pobj	epithelium,in
R1339	T2218	T2170	punct	.,provide
R1340	T2073	T2071	pobj	growth,during
R1341	T2220	T2221	prep	In,form
R1342	T2074	T2073	nmod	follicle,growth
R1343	T2222	T2223	det	the,absence
R1344	T2223	T2220	pobj	absence,In
R1345	T2075	T2073	amod	down,growth
R1346	T2224	T2223	prep	of,absence
R1347	T2225	T2226	compound	TGF,β2
R1348	T2226	T2228	compound	β2,signaling
R1349	T2076	T2073	punct	-,growth
R1350	T2227	T2226	punct	-,β2
R1351	T2077	T2073	cc	and,growth
R1352	T2228	T2224	pobj	signaling,of
R1353	T2229	T2228	cc	and,signaling
R1354	T2230	T2231	compound	Snail,expression
R1355	T2078	T2073	conj	maturation,growth
R1356	T2231	T2228	conj	expression,signaling
R1357	T2232	T2231	compound	gene,expression
R1358	T2079	T2065	punct	.,disappears
R1359	T2233	T2221	punct	", ",form
R1360	T2234	T2235	compound	hair,placodes
R1361	T2235	T2221	nsubj	placodes,form
R1362	T2236	T2221	aux	can,form
R1363	T2237	T2221	punct	", ",form
R1364	T2238	T2221	cc	but,form
R1365	T2081	T2082	det	This,pattern
R1366	T2239	T2240	amod	further,growth
R1367	T2240	T2244	nsubjpass	growth,blocked
R1368	T2241	T2240	nmod	follicle,growth
R1369	T2242	T2240	amod	down,growth
R1370	T2082	T2084	nsubj	pattern,appears
R1371	T2243	T2240	punct	-,growth
R1372	T2244	T2221	conj	blocked,form
R1373	T2245	T2244	auxpass	is,blocked
R1374	T2083	T2082	amod	exquisite,pattern
R1375	T2246	T2221	punct	.,form
R1376	T2085	T2086	aux	to,be
R1377	T2248	T2249	poss	Our,studies
R1378	T2249	T2250	nsubj	studies,point
R1379	T2251	T2250	prep	to,point
R1380	T2086	T2084	xcomp	be,appears
R1381	T2252	T2253	det	the,view
R1382	T2253	T2251	pobj	view,to
R1383	T2087	T2088	advmod	functionally,relevant
R1384	T2254	T2255	mark	that,functions
R1385	T2255	T2253	acl	functions,view
R1386	T2256	T2255	nsubj	Snail,functions
R1387	T2088	T2086	acomp	relevant,be
R1388	T2257	T2255	advmod	likely,functions
R1389	T2258	T2255	advmod	downstream,functions
R1390	T2259	T2258	prep	of,downstream
R1391	T2089	T2090	mark	since,affects
R1392	T2260	T2261	compound	cell,fate
R1393	T2261	T2262	compound	fate,specification
R1394	T2090	T2086	advcl	affects,be
R1395	T2262	T2259	pobj	specification,of
R1396	T2263	T2250	punct	", ",point
R1397	T2264	T2250	prep	at,point
R1398	T2091	T2090	csubj	altering,affects
R1399	T2265	T2266	det	a,stage
R1400	T2266	T2264	pobj	stage,at
R1401	T2092	T2091	dobj	it,altering
R1402	T2267	T2268	advmod	where,begins
R1403	T2268	T2266	relcl	begins,stage
R1404	T2093	T2094	advmod	in,vivo
R1405	T2269	T2270	det	the,bud
R1406	T2270	T2268	nsubj	bud,begins
R1407	T2094	T2091	advmod	vivo,altering
R1408	T2271	T2272	aux	to,exhibit
R1409	T2272	T2268	xcomp	exhibit,begins
R1410	T2273	T2274	amod	enhanced,proliferation
R1411	T2095	T2090	advmod	correspondingly,affects
R1412	T2274	T2272	dobj	proliferation,exhibit
R1413	T2275	T2274	cc	and,proliferation
R1414	T2276	T2274	conj	migration,proliferation
R1415	T2277	T2250	punct	.,point
R1416	T2096	T2090	dobj	features,affects
R1433	T2702	T2703	compound	Snail,mRNA
R1434	T2703	T2704	nsubjpass	mRNA,Expressed
R1435	T2705	T2703	cc	and,mRNA
R1436	T2706	T2703	conj	Protein,mRNA
R1437	T2707	T2704	auxpass	Are,Expressed
R1438	T2708	T2704	advmod	Transiently,Expressed
R1439	T2709	T2704	prep	at,Expressed
R1440	T2710	T2711	det	the,Stage
R1441	T2711	T2709	pobj	Stage,at
R1442	T2712	T2713	compound	Hair,Bud
R1443	T2713	T2711	compound	Bud,Stage
R1444	T2714	T2711	prep	of,Stage
R1445	T2715	T2716	compound	Follicle,Morphogenesis
R1446	T2716	T2714	pobj	Morphogenesis,of
R1447	T2718	T2719	mark	Although,associated
R1448	T2719	T2726	advcl	associated,participate
R1449	T2720	T2721	compound	Snail,members
R1450	T2721	T2719	nsubjpass	members,associated
R1451	T2722	T2721	compound	family,members
R1452	T2723	T2719	auxpass	are,associated
R1453	T2724	T2725	advmod	most,frequently
R1454	T2725	T2719	advmod	frequently,associated
R1455	T2727	T2719	prep	with,associated
R1456	T2728	T2727	pobj	EMTs,with
R1457	T2729	T2726	punct	", ",participate
R1458	T2730	T2726	nsubj	they,participate
R1459	T2731	T2726	advmod	also,participate
R1460	T2732	T2726	prep	in,participate
R1461	T2733	T2734	amod	many,processes
R1462	T2734	T2732	pobj	processes,in
R1463	T2735	T2734	amod	malignant,processes
R1464	T2736	T2734	acl	involving,processes
R1465	T2737	T2738	det	a,regulation
R1466	T2738	T2736	dobj	regulation,involving
R1467	T2739	T2738	amod	down,regulation
R1468	T2740	T2738	punct	-,regulation
R1469	T2741	T2738	cc	but,regulation
R1470	T2742	T2741	neg	not,but
R1471	T2743	T2744	det	a,abrogation
R1472	T2744	T2738	conj	abrogation,regulation
R1473	T2745	T2744	amod	quantitative,abrogation
R1474	T2746	T2738	prep	of,regulation
R1475	T2747	T2748	amod	intercellular,junctions
R1476	T2748	T2746	pobj	junctions,of
R1477	T2749	T2750	punct	[,18
R1478	T2750	T2726	parataxis	18,participate
R1479	T2751	T2750	punct	],18
R1480	T2752	T2726	punct	.,participate
R1481	T2754	T2755	det	The,range
R1482	T2755	T2756	nsubj	range,includes
R1483	T2757	T2755	prep	of,range
R1484	T2758	T2759	amod	developmental,processes
R1485	T2759	T2757	pobj	processes,of
R1486	T2760	T2761	prep	in,implicated
R1487	T2761	T2759	relcl	implicated,processes
R1488	T2762	T2760	pobj	which,in
R1489	T2763	T2764	compound	Snail,members
R1490	T2764	T2761	nsubjpass	members,implicated
R1491	T2765	T2764	compound	family,members
R1492	T2766	T2761	aux	have,implicated
R1493	T2767	T2761	auxpass	been,implicated
R1494	T2768	T2756	advmod	thus,includes
R1495	T2769	T2770	det	the,type
R1496	T2770	T2756	dobj	type,includes
R1497	T2771	T2770	prep	of,type
R1498	T2772	T2773	amod	epithelial,remodeling
R1499	T2773	T2771	pobj	remodeling,of
R1500	T2774	T2775	dep	that,observed
R1501	T2775	T2770	relcl	observed,type
R1502	T2776	T2775	auxpass	is,observed
R1503	T2777	T2775	prep	in,observed
R1504	T2778	T2779	compound	hair,follicle
R1505	T2779	T2780	compound	follicle,bud
R1506	T2780	T2781	compound	bud,formation
R1507	T2781	T2777	pobj	formation,in
R1508	T2782	T2756	punct	.,includes
R1509	T2784	T2785	prep	Given,turned
R1510	T2786	T2787	poss	our,observation
R1511	T2787	T2784	pobj	observation,Given
R1512	T2788	T2787	amod	prior,observation
R1513	T2789	T2790	mark	that,participate
R1514	T2790	T2787	acl	participate,observation
R1515	T2791	T2792	advmod	exogenously,added
R1516	T2792	T2793	amod	added,Snail
R1517	T2793	T2790	nsubj	Snail,participate
R1518	T2794	T2790	aux	can,participate
R1519	T2795	T2790	prep	with,participate
R1520	T2796	T2795	pobj	LEF,with
R1521	T2797	T2796	punct	-,LEF
R1522	T2798	T2796	nummod	1,LEF
R1523	T2799	T2796	punct	/,LEF
R1524	T2800	T2801	compound	β,catenin
R1525	T2801	T2796	appos	catenin,LEF
R1526	T2802	T2801	punct	-,catenin
R1527	T2803	T2790	prep	in,participate
R1528	T2804	T2805	advmod	down,regulating
R1529	T2805	T2803	pcomp	regulating,in
R1530	T2806	T2805	punct	-,regulating
R1531	T2807	T2808	compound	E,cadherin
R1532	T2808	T2810	compound	cadherin,expression
R1533	T2809	T2808	punct	-,cadherin
R1534	T2810	T2805	dobj	expression,regulating
R1535	T2811	T2790	prep	in,participate
R1536	T2812	T2811	pobj	keratinocytes,in
R1537	T2813	T2814	punct	[,4
R1538	T2814	T2790	parataxis	4,participate
R1539	T2815	T2814	punct	],4
R1540	T2816	T2787	punct	", ",observation
R1541	T2817	T2787	acl	coupled,observation
R1542	T2818	T2817	prep	with,coupled
R1543	T2819	T2820	det	the,requirement
R1544	T2820	T2818	pobj	requirement,with
R1545	T2821	T2820	amod	established,requirement
R1546	T2822	T2820	prep	for,requirement
R1547	T2823	T2822	pobj	LEF,for
R1548	T2824	T2823	punct	-,LEF
R1549	T2825	T2823	nummod	1,LEF
R1550	T2826	T2823	punct	/,LEF
R1551	T2827	T2828	compound	β,catenin
R1552	T2828	T2823	appos	catenin,LEF
R1553	T2829	T2828	punct	-,catenin
R1554	T2830	T2820	prep	in,requirement
R1555	T2831	T2832	compound	hair,follicle
R1556	T2832	T2833	compound	follicle,morphogenesis
R1557	T2833	T2830	pobj	morphogenesis,in
R1558	T2834	T2835	punct	[,19
R1559	T2835	T2817	parataxis	19,coupled
R1560	T2836	T2835	nummod	4,19
R1561	T2837	T2835	punct	",",19
R1562	T2838	T2835	punct	],19
R1563	T2839	T2785	punct	", ",turned
R1564	T2840	T2785	nsubj	we,turned
R1565	T2841	T2785	prep	to,turned
R1566	T2842	T2841	pcomp	addressing,to
R1567	T2843	T2844	mark	whether,participate
R1568	T2844	T2842	ccomp	participate,addressing
R1569	T2845	T2846	compound	Snail,Slug
R1570	T2846	T2848	compound	Slug,members
R1571	T2847	T2846	punct	/,Slug
R1572	T2848	T2844	nsubj	members,participate
R1573	T2849	T2848	compound	family,members
R1574	T2850	T2844	aux	might,participate
R1575	T2851	T2844	advmod	also,participate
R1576	T2852	T2844	prep	in,participate
R1577	T2853	T2854	det	the,process
R1578	T2854	T2852	pobj	process,in
R1579	T2855	T2785	punct	.,turned
R1580	T2857	T2858	compound	PCR,analyses
R1581	T2858	T2859	nsubj	analyses,identified
R1582	T2860	T2861	amod	transient,expression
R1583	T2861	T2859	dobj	expression,identified
R1584	T2862	T2861	compound	Snail,expression
R1585	T2863	T2861	compound	mRNA,expression
R1586	T2864	T2859	prep	during,identified
R1587	T2865	T2866	det	a,period
R1588	T2866	T2864	pobj	period,during
R1589	T2867	T2866	prep	of,period
R1590	T2868	T2869	compound	skin,embryogenesis
R1591	T2869	T2867	pobj	embryogenesis,of
R1592	T2870	T2871	advmod	when,forming
R1593	T2871	T2866	advcl	forming,period
R1594	T2872	T2871	nsubj	waves,forming
R1595	T2873	T2872	prep	of,waves
R1596	T2874	T2875	compound	hair,follicles
R1597	T2875	T2873	pobj	follicles,of
R1598	T2876	T2871	aux	are,forming
R1599	T2877	T2878	punct	(,data
R1600	T2878	T2859	meta	data,identified
R1601	T2879	T2878	amod	unpublished,data
R1602	T2880	T2878	punct	),data
R1603	T2881	T2859	punct	.,identified
R1604	T2882	T2883	aux	To,pinpoint
R1605	T2883	T2885	advcl	pinpoint,conducted
R1606	T2886	T2883	advmod	specifically,pinpoint
R1607	T2887	T2888	advmod	where,expressed
R1608	T2888	T2883	advcl	expressed,pinpoint
R1609	T2889	T2890	compound	Snail,mRNA
R1610	T2890	T2888	nsubjpass	mRNA,expressed
R1611	T2891	T2888	auxpass	is,expressed
R1612	T2892	T2888	prep	in,expressed
R1613	T2893	T2894	det	the,skin
R1614	T2894	T2892	pobj	skin,in
R1615	T2895	T2894	amod	developing,skin
R1616	T2896	T2885	punct	", ",conducted
R1617	T2897	T2885	nsubj	we,conducted
R1618	T2898	T2899	advmod	in,situ
R1619	T2899	T2900	amod	situ,hybridization
R1620	T2900	T2885	dobj	hybridization,conducted
R1621	T2901	T2885	advcl	using,conducted
R1622	T2902	T2903	det	a,probe
R1623	T2903	T2901	dobj	probe,using
R1624	T2904	T2903	compound	cRNA,probe
R1625	T2905	T2903	amod	unique,probe
R1626	T2906	T2905	prep	to,unique
R1627	T2907	T2908	det	the,region
R1628	T2908	T2906	pobj	region,to
R1629	T2909	T2908	nmod	Snail,region
R1630	T2910	T2908	nummod	3,region
R1631	T2911	T2910	punct	′,3
R1632	T2912	T2908	amod	untranslated,region
R1633	T2913	T2908	punct	(,region
R1634	T2914	T2908	appos	UTR,region
R1635	T2915	T2908	punct	),region
R1636	T2916	T2885	punct	.,conducted
R1637	T2918	T2919	amod	Embryonic,day
R1638	T2919	T2920	nsubjpass	day,chosen
R1639	T2921	T2919	nummod	17.5,day
R1640	T2922	T2919	punct	(,day
R1641	T2923	T2919	appos	E17.5,day
R1642	T2924	T2920	punct	),chosen
R1643	T2925	T2920	auxpass	was,chosen
R1644	T2926	T2920	punct	", ",chosen
R1645	T2927	T2928	mark	since,enabled
R1646	T2928	T2920	advcl	enabled,chosen
R1647	T2929	T2930	det	the,waves
R1648	T2930	T2928	nsubj	waves,enabled
R1649	T2931	T2930	amod	multiple,waves
R1650	T2932	T2930	prep	of,waves
R1651	T2933	T2934	compound	follicle,morphogenesis
R1652	T2934	T2932	pobj	morphogenesis,of
R1653	T2935	T2930	acl	occurring,waves
R1654	T2936	T2935	prep	at,occurring
R1655	T2937	T2938	det	this,time
R1656	T2938	T2936	pobj	time,at
R1657	T2939	T2928	dobj	us,enabled
R1658	T2940	T2941	aux	to,evaluate
R1659	T2941	T2928	xcomp	evaluate,enabled
R1660	T2942	T2943	compound	Snail,expression
R1661	T2943	T2941	dobj	expression,evaluate
R1662	T2944	T2941	prep	at,evaluate
R1663	T2945	T2946	amod	different,stages
R1664	T2946	T2944	pobj	stages,at
R1665	T2947	T2946	prep	of,stages
R1666	T2948	T2949	det	the,process
R1667	T2949	T2947	pobj	process,of
R1668	T2950	T2920	punct	.,chosen
R1669	T2952	T2953	mark	As,shown
R1670	T2953	T2954	advcl	shown,detected
R1671	T2955	T2953	prep	in,shown
R1672	T2956	T2957	compound	Figure,1A
R1673	T2957	T2955	pobj	1A,in
R1674	T2958	T2954	punct	", ",detected
R1675	T2959	T2960	amod	specific,hybridization
R1676	T2960	T2954	nsubjpass	hybridization,detected
R1677	T2961	T2954	auxpass	was,detected
R1678	T2962	T2954	prep	within,detected
R1679	T2963	T2964	det	the,epithelium
R1680	T2964	T2962	pobj	epithelium,within
R1681	T2965	T2964	prep	of,epithelium
R1682	T2966	T2967	amod	nascent,buds
R1683	T2967	T2965	pobj	buds,of
R1684	T2968	T2967	compound	hair,buds
R1685	T2969	T2954	punct	.,detected
R1686	T2971	T2972	prep	By,exhibited
R1687	T2973	T2971	pobj	contrast,By
R1688	T2974	T2972	punct	", ",exhibited
R1689	T2975	T2976	mark	as,progressed
R1690	T2976	T2972	advcl	progressed,exhibited
R1691	T2977	T2976	nsubj	follicles,progressed
R1692	T2978	T2976	advmod	further,progressed
R1693	T2979	T2976	prep	through,progressed
R1694	T2980	T2981	poss	their,development
R1695	T2981	T2979	pobj	development,through
R1696	T2982	T2983	punct	(,stages
R1697	T2983	T2976	parataxis	stages,progressed
R1698	T2984	T2983	advmod	e.g.,stages
R1699	T2985	T2983	punct	", ",stages
R1700	T2986	T2983	nmod	germ,stages
R1701	T2987	T2986	cc	and,germ
R1702	T2988	T2986	conj	peg,germ
R1703	T2989	T2983	punct	),stages
R1704	T2990	T2972	punct	", ",exhibited
R1705	T2991	T2972	nsubj	they,exhibited
R1706	T2992	T2993	det	no,signs
R1707	T2993	T2972	dobj	signs,exhibited
R1708	T2994	T2993	prep	of,signs
R1709	T2995	T2994	pobj	hybridization,of
R1710	T2996	T2997	punct	(,1A
R1711	T2997	T2972	parataxis	1A,exhibited
R1712	T2998	T2997	compound	Figure,1A
R1713	T2999	T2997	punct	),1A
R1714	T3000	T2972	punct	.,exhibited
R1715	T3002	T3003	det	The,nature
R1716	T3003	T3005	nsubj	nature,was
R1717	T3004	T3003	amod	transient,nature
R1718	T3006	T3003	prep	of,nature
R1719	T3007	T3008	compound	Snail,expression
R1720	T3008	T3006	pobj	expression,of
R1721	T3009	T3008	compound	mRNA,expression
R1722	T3010	T3008	prep	during,expression
R1723	T3011	T3012	compound	follicle,development
R1724	T3012	T3010	pobj	development,during
R1725	T3013	T3014	advmod	most,apparent
R1726	T3014	T3005	acomp	apparent,was
R1727	T3015	T3005	prep	in,was
R1728	T3016	T3017	amod	hybridized,sections
R1729	T3017	T3015	pobj	sections,in
R1730	T3018	T3017	compound	skin,sections
R1731	T3019	T3017	acl	containing,sections
R1732	T3020	T3019	dobj	follicles,containing
R1733	T3021	T3020	prep	from,follicles
R1734	T3022	T3023	nummod	two,waves
R1735	T3023	T3021	pobj	waves,from
R1736	T3024	T3023	amod	different,waves
R1737	T3025	T3023	prep	of,waves
R1738	T3026	T3025	pobj	morphogenesis,of
R1739	T3027	T3028	punct	(,shown
R1740	T3028	T3005	parataxis	shown,was
R1741	T3029	T3028	mark	as,shown
R1742	T3030	T3028	prep	in,shown
R1743	T3031	T3030	pobj	Figure,in
R1744	T3032	T3031	nummod	1,Figure
R1745	T3033	T3028	punct	),shown
R1746	T3034	T3005	punct	.,was
R1747	T3036	T3037	amod	Hybridizing,buds
R1748	T3037	T3039	nsubj	buds,appeared
R1749	T3038	T3037	compound	hair,buds
R1750	T3040	T3037	prep	from,buds
R1751	T3041	T3042	det	a,wave
R1752	T3042	T3040	pobj	wave,from
R1753	T3043	T3042	amod	later,wave
R1754	T3044	T3039	oprd	juxtaposed,appeared
R1755	T3045	T3044	prep	with,juxtaposed
R1756	T3046	T3047	amod	nonhybridizing,follicles
R1757	T3047	T3045	pobj	follicles,with
R1758	T3048	T3047	prep	from,follicles
R1759	T3049	T3050	det	an,wave
R1760	T3050	T3048	pobj	wave,from
R1761	T3051	T3050	amod	earlier,wave
R1762	T3052	T3039	punct	.,appeared
R1763	T3054	T3055	aux	To,determine
R1764	T3055	T3056	advcl	determine,generated
R1765	T3057	T3058	mark	whether,reflected
R1766	T3058	T3055	ccomp	reflected,determine
R1767	T3059	T3060	det	this,nature
R1768	T3060	T3058	nsubjpass	nature,reflected
R1769	T3061	T3060	amod	transient,nature
R1770	T3062	T3060	prep	of,nature
R1771	T3063	T3064	compound	Snail,expression
R1772	T3064	T3062	pobj	expression,of
R1773	T3065	T3064	compound	mRNA,expression
R1774	T3066	T3058	auxpass	is,reflected
R1775	T3067	T3058	prep	at,reflected
R1776	T3068	T3069	det	the,level
R1777	T3069	T3067	pobj	level,at
R1778	T3070	T3069	compound	protein,level
R1779	T3071	T3056	punct	", ",generated
R1780	T3072	T3056	nsubj	we,generated
R1781	T3073	T3074	det	an,antibody
R1782	T3074	T3056	dobj	antibody,generated
R1783	T3075	T3074	prep	against,antibody
R1784	T3076	T3077	det	the,sequence
R1785	T3077	T3075	pobj	sequence,against
R1786	T3078	T3079	npadvmod	N,terminal
R1787	T3079	T3077	amod	terminal,sequence
R1788	T3080	T3079	punct	-,terminal
R1789	T3081	T3082	dep	that,resides
R1790	T3082	T3077	relcl	resides,sequence
R1791	T3083	T3082	advmod	upstream,resides
R1792	T3084	T3083	prep	of,upstream
R1793	T3085	T3086	det	the,domains
R1794	T3086	T3084	pobj	domains,of
R1795	T3087	T3088	advmod	more,conserved
R1796	T3088	T3086	amod	conserved,domains
R1797	T3089	T3090	compound	zinc,finger
R1798	T3090	T3086	compound	finger,domains
R1799	T3091	T3056	punct	.,generated
R1800	T3093	T3094	mark	As,judged
R1801	T3094	T3095	advcl	judged,detect
R1802	T3096	T3094	prep	by,judged
R1803	T3097	T3098	compound	Western,blot
R1804	T3098	T3099	compound	blot,analysis
R1805	T3099	T3096	pobj	analysis,by
R1806	T3100	T3095	punct	", ",detect
R1807	T3101	T3102	det	the,antibody
R1808	T3102	T3095	nsubj	antibody,detect
R1809	T3103	T3095	aux	did,detect
R1810	T3104	T3095	neg	not,detect
R1811	T3105	T3106	amod	endogenous,proteins
R1812	T3106	T3095	dobj	proteins,detect
R1813	T3107	T3106	prep	from,proteins
R1814	T3108	T3109	amod	cultured,keratinocytes
R1815	T3109	T3107	pobj	keratinocytes,from
R1816	T3110	T3095	punct	", ",detect
R1817	T3111	T3095	cc	but,detect
R1818	T3112	T3113	nsubj	it,yield
R1819	T3113	T3095	conj	yield,detect
R1820	T3114	T3113	aux	did,yield
R1821	T3115	T3116	det	a,band
R1822	T3116	T3113	dobj	band,yield
R1823	T3117	T3116	prep	of,band
R1824	T3118	T3119	det	the,size
R1825	T3119	T3117	pobj	size,of
R1826	T3120	T3119	amod	expected,size
R1827	T3121	T3113	prep	from,yield
R1828	T3122	T3121	pobj	keratinocytes,from
R1829	T3123	T3124	advmod	transiently,expressing
R1830	T3124	T3122	acl	expressing,keratinocytes
R1831	T3125	T3126	det	a,protein
R1832	T3126	T3124	dobj	protein,expressing
R1833	T3127	T3128	npadvmod	hemagglutinin,tagged
R1834	T3128	T3126	amod	tagged,protein
R1835	T3129	T3127	punct	(,hemagglutinin
R1836	T3130	T3127	appos	HA,hemagglutinin
R1837	T3131	T3128	punct	),tagged
R1838	T3132	T3128	punct	-,tagged
R1839	T3133	T3126	compound	Snail,protein
R1840	T3134	T3135	punct	(,1B
R1841	T3135	T3113	parataxis	1B,yield
R1842	T3136	T3135	compound	Figure,1B
R1843	T3137	T3135	punct	),1B
R1844	T3138	T3095	punct	.,detect
R1845	T3140	T3141	det	The,antibody
R1846	T3141	T3142	nsubj	antibody,recognized
R1847	T3143	T3142	advmod	also,recognized
R1848	T3144	T3145	det	a,band
R1849	T3145	T3142	dobj	band,recognized
R1850	T3146	T3145	acl	corresponding,band
R1851	T3147	T3146	prep	to,corresponding
R1852	T3148	T3149	det	the,size
R1853	T3149	T3147	pobj	size,to
R1854	T3150	T3149	prep	of,size
R1855	T3151	T3152	amod	endogenous,Snail
R1856	T3152	T3150	pobj	Snail,of
R1857	T3153	T3149	punct	(,size
R1858	T3154	T3155	advmod	approximately,28
R1859	T3155	T3156	nummod	28,kDa
R1860	T3156	T3149	appos	kDa,size
R1861	T3157	T3149	punct	),size
R1862	T3158	T3149	prep	in,size
R1863	T3159	T3158	pobj	lysates,in
R1864	T3160	T3159	prep	from,lysates
R1865	T3161	T3162	amod	embryonic,skin
R1866	T3162	T3160	pobj	skin,from
R1867	T3163	T3162	compound	mouse,skin
R1868	T3164	T3145	punct	", ",band
R1869	T3165	T3166	det	the,appearance
R1870	T3166	T3168	dep	appearance,corresponded
R1871	T3167	T3166	amod	temporal,appearance
R1872	T3168	T3145	relcl	corresponded,band
R1873	T3169	T3166	prep	of,appearance
R1874	T3170	T3169	pobj	which,of
R1875	T3171	T3168	prep	to,corresponded
R1876	T3172	T3173	det	the,waves
R1877	T3173	T3171	pobj	waves,to
R1878	T3174	T3173	prep	of,waves
R1879	T3175	T3176	compound	hair,follicle
R1880	T3176	T3177	compound	follicle,morphogenesis
R1881	T3177	T3174	pobj	morphogenesis,of
R1882	T3178	T3173	prep	from,waves
R1883	T3179	T3178	pobj	E15.5,from
R1884	T3180	T3178	prep	to,from
R1885	T3181	T3180	amod	newborn,to
R1886	T3182	T3183	advmod	when,formed
R1887	T3183	T3173	relcl	formed,waves
R1888	T3184	T3185	quantmod	over,90
R1889	T3185	T3186	nummod	90,%
R1890	T3186	T3183	nsubjpass	%,formed
R1891	T3187	T3186	prep	of,%
R1892	T3188	T3189	det	the,hair
R1893	T3189	T3187	pobj	hair,of
R1894	T3190	T3189	prep	on,hair
R1895	T3191	T3192	det	the,mouse
R1896	T3192	T3190	pobj	mouse,on
R1897	T3193	T3183	auxpass	is,formed
R1898	T3194	T3195	punct	(,1B
R1899	T3195	T3142	parataxis	1B,recognized
R1900	T3196	T3195	compound	Figure,1B
R1901	T3197	T3195	punct	),1B
R1902	T3198	T3142	punct	.,recognized
R1903	T3200	T3201	advcl	Consistent,detect
R1904	T3202	T3200	prep	with,Consistent
R1905	T3203	T3204	det	the,data
R1906	T3204	T3202	pobj	data,with
R1907	T3205	T3206	compound	Western,blot
R1908	T3206	T3204	compound	blot,data
R1909	T3207	T3201	punct	", ",detect
R1910	T3208	T3209	amod	immunohistochemical,analysis
R1911	T3209	T3201	nsubj	analysis,detect
R1912	T3210	T3201	aux	did,detect
R1913	T3211	T3201	neg	not,detect
R1914	T3212	T3201	dobj	Snail,detect
R1915	T3213	T3201	prep	in,detect
R1916	T3214	T3215	amod	single,layered
R1917	T3215	T3217	amod	layered,epidermis
R1918	T3216	T3215	punct	-,layered
R1919	T3217	T3213	pobj	epidermis,in
R1920	T3218	T3217	compound	E13.5,epidermis
R1921	T3219	T3220	punct	(,1C
R1922	T3220	T3217	parataxis	1C,epidermis
R1923	T3221	T3220	compound	Figure,1C
R1924	T3222	T3220	punct	),1C
R1925	T3223	T3213	cc	nor,in
R1926	T3224	T3213	conj	in,in
R1927	T3225	T3226	det	the,placode
R1928	T3226	T3224	pobj	placode,in
R1929	T3227	T3201	punct	", ",detect
R1930	T3228	T3229	dep	which,is
R1931	T3229	T3201	advcl	is,detect
R1932	T3230	T3231	det	the,sign
R1933	T3231	T3229	attr	sign,is
R1934	T3232	T3231	amod	earliest,sign
R1935	T3233	T3231	amod	morphological,sign
R1936	T3234	T3231	prep	of,sign
R1937	T3235	T3236	det	the,commitment
R1938	T3236	T3234	pobj	commitment,of
R1939	T3237	T3236	prep	of,commitment
R1940	T3238	T3239	amod	multipotent,cells
R1941	T3239	T3237	pobj	cells,of
R1942	T3240	T3239	prep	of,cells
R1943	T3241	T3242	det	the,ectoderm
R1944	T3242	T3240	pobj	ectoderm,of
R1945	T3243	T3242	amod	embryonic,ectoderm
R1946	T3244	T3236	prep	to,commitment
R1947	T3245	T3246	det	a,fate
R1948	T3246	T3244	pobj	fate,to
R1949	T3247	T3248	compound	hair,cell
R1950	T3248	T3246	compound	cell,fate
R1951	T3249	T3250	punct	(,1D
R1952	T3250	T3201	parataxis	1D,detect
R1953	T3251	T3250	compound	Figure,1D
R1954	T3252	T3250	punct	),1D
R1955	T3253	T3201	punct	.,detect
R1956	T3255	T3256	advcl	Consistent,labeled
R1957	T3257	T3255	prep	with,Consistent
R1958	T3258	T3259	det	the,results
R1959	T3259	T3257	pobj	results,with
R1960	T3260	T3261	advmod	in,situ
R1961	T3261	T3259	amod	situ,results
R1962	T3262	T3259	compound	hybridization,results
R1963	T3263	T3256	punct	", ",labeled
R1964	T3264	T3265	amod	anti-Snail,antibody
R1965	T3265	T3256	nsubj	antibody,labeled
R1966	T3266	T3267	advmod	only,buds
R1967	T3267	T3256	dobj	buds,labeled
R1968	T3268	T3267	compound	hair,buds
R1969	T3269	T3267	cc	and,buds
R1970	T3270	T3269	neg	not,and
R1971	T3271	T3267	conj	follicles,buds
R1972	T3272	T3256	prep	at,labeled
R1973	T3273	T3274	advmod	more,stages
R1974	T3274	T3272	pobj	stages,at
R1975	T3275	T3274	amod	mature,stages
R1976	T3276	T3274	prep	of,stages
R1977	T3277	T3276	pobj	development,of
R1978	T3278	T3279	punct	(,1E
R1979	T3279	T3256	parataxis	1E,labeled
R1980	T3280	T3279	compound	Figure,1E
R1981	T3281	T3279	punct	),1E
R1982	T3282	T3256	punct	.,labeled
R1983	T3284	T3285	advcl	Taken,appeared
R1984	T3286	T3284	advmod	together,Taken
R1985	T3287	T3285	punct	", ",appeared
R1986	T3288	T3289	det	the,antibody
R1987	T3289	T3285	nsubj	antibody,appeared
R1988	T3290	T3289	amod	anti-Snail,antibody
R1989	T3291	T3292	aux	to,be
R1990	T3292	T3285	xcomp	be,appeared
R1991	T3293	T3292	acomp	specific,be
R1992	T3294	T3293	prep	for,specific
R1993	T3295	T3296	poss	its,protein
R1994	T3296	T3294	pobj	protein,for
R1995	T3297	T3296	compound	target,protein
R1996	T3298	T3285	punct	.,appeared
R1997	T3300	T3301	nsubj	It,detect
R1998	T3302	T3301	aux	did,detect
R1999	T3303	T3301	neg	not,detect
R2000	T3304	T3305	amod	other,members
R2001	T3305	T3301	dobj	members,detect
R2002	T3306	T3305	compound	Snail,members
R2003	T3307	T3305	compound	family,members
R2004	T3308	T3305	acl	known,members
R2005	T3309	T3310	aux	to,expressed
R2006	T3310	T3308	xcomp	expressed,known
R2007	T3311	T3310	auxpass	be,expressed
R2008	T3312	T3310	prep	in,expressed
R2009	T3313	T3312	pobj	keratinocytes,in
R2010	T3314	T3313	cc	and,keratinocytes
R2011	T3315	T3314	punct	/,and
R2012	T3316	T3314	cc	or,and
R2013	T3317	T3313	conj	skin,keratinocytes
R2014	T3318	T3319	punct	(,data
R2015	T3319	T3308	meta	data,known
R2016	T3320	T3319	amod	unpublished,data
R2017	T3321	T3319	punct	),data
R2018	T3322	T3301	punct	.,detect
R2019	T3324	T3325	advmod	Furthermore,paralleled
R2020	T3326	T3325	punct	", ",paralleled
R2021	T3327	T3328	det	the,data
R2022	T3328	T3325	nsubj	data,paralleled
R2023	T3329	T3328	amod	immunohistochemical,data
R2024	T3330	T3331	poss	our,data
R2025	T3331	T3325	dobj	data,paralleled
R2026	T3332	T3333	nmod	Snail,hybridization
R2027	T3333	T3331	compound	hybridization,data
R2028	T3334	T3335	advmod	in,situ
R2029	T3335	T3333	amod	situ,hybridization
R2030	T3336	T3325	advcl	revealing,paralleled
R2031	T3337	T3338	amod	transient,expression
R2032	T3338	T3336	dobj	expression,revealing
R2033	T3339	T3338	compound	Snail,expression
R2034	T3340	T3336	prep	at,revealing
R2035	T3341	T3342	det	the,stage
R2036	T3342	T3340	pobj	stage,at
R2037	T3343	T3344	compound	hair,bud
R2038	T3344	T3342	compound	bud,stage
R2039	T3345	T3346	punct	(,1A
R2040	T3346	T3325	parataxis	1A,paralleled
R2041	T3347	T3346	compound	Figure,1A
R2042	T3348	T3346	punct	),1A
R2043	T3349	T3325	punct	.,paralleled
R2044	T3351	T3352	mark	As,judged
R2045	T3352	T3353	advcl	judged,localized
R2046	T3354	T3352	prep	by,judged
R2047	T3355	T3354	pobj	immunohistochemistry,by
R2048	T3356	T3353	punct	", ",localized
R2049	T3357	T3358	compound	Snail,protein
R2050	T3358	T3353	nsubjpass	protein,localized
R2051	T3359	T3353	auxpass	was,localized
R2052	T3360	T3353	prep	to,localized
R2053	T3361	T3362	det	the,nuclei
R2054	T3362	T3360	pobj	nuclei,to
R2055	T3363	T3362	prep	of,nuclei
R2056	T3364	T3365	det	the,cells
R2057	T3365	T3363	pobj	cells,of
R2058	T3366	T3367	compound	hair,bud
R2059	T3367	T3365	compound	bud,cells
R2060	T3368	T3369	punct	(,1E
R2061	T3369	T3353	parataxis	1E,localized
R2062	T3370	T3369	compound	Figure,1E
R2063	T3371	T3369	punct	),1E
R2064	T3372	T3353	punct	.,localized
R2065	T3374	T3375	det	This,feature
R2066	T3375	T3376	nsubj	feature,was
R2067	T3377	T3376	acomp	consistent,was
R2068	T3378	T3377	prep	with,consistent
R2069	T3379	T3380	poss	Snail,function
R2070	T3380	T3378	pobj	function,with
R2071	T3381	T3379	case	's,Snail
R2072	T3382	T3380	amod	known,function
R2073	T3383	T3380	prep	as,function
R2074	T3384	T3385	det	a,repressor
R2075	T3385	T3383	pobj	repressor,as
R2076	T3386	T3385	amod	transcriptional,repressor
R2077	T3387	T3388	punct	[,13
R2078	T3388	T3376	parataxis	13,was
R2079	T3389	T3388	nummod	12,13
R2080	T3390	T3388	punct	",",13
R2081	T3391	T3388	punct	],13
R2082	T3392	T3376	punct	.,was
R2083	T3394	T3395	advmod	Additionally,detected
R2084	T3396	T3395	punct	", ",detected
R2085	T3397	T3398	amod	anti-Snail,labeling
R2086	T3398	T3395	nsubjpass	labeling,detected
R2087	T3399	T3395	auxpass	was,detected
R2088	T3400	T3395	prep	in,detected
R2089	T3401	T3402	advmod	only,four
R2090	T3402	T3406	nummod	four,waves
R2091	T3403	T3402	quantmod	three,four
R2092	T3404	T3402	quantmod	of,four
R2093	T3405	T3402	quantmod	the,four
R2094	T3406	T3400	pobj	waves,in
R2095	T3407	T3406	amod	major,waves
R2096	T3408	T3406	prep	of,waves
R2097	T3409	T3410	compound	follicle,morphogenesis
R2098	T3410	T3408	pobj	morphogenesis,of
R2099	T3411	T3395	punct	.,detected
R2100	T3413	T3414	nsubjpass	Snail,found
R2101	T3415	T3414	auxpass	was,found
R2102	T3416	T3414	neg	not,found
R2103	T3417	T3414	prep	in,found
R2104	T3418	T3419	det	the,buds
R2105	T3419	T3417	pobj	buds,in
R2106	T3420	T3419	prep	of,buds
R2107	T3421	T3422	compound	guard,hairs
R2108	T3422	T3420	pobj	hairs,of
R2109	T3423	T3424	dep	that,are
R2110	T3424	T3422	advcl	are,hairs
R2111	T3425	T3426	det	the,earliest
R2112	T3426	T3424	attr	earliest,are
R2113	T3427	T3426	prep	of,earliest
R2114	T3428	T3429	det	all,hairs
R2115	T3429	T3427	pobj	hairs,of
R2116	T3430	T3431	aux	to,form
R2117	T3431	T3429	advcl	form,hairs
R2118	T3432	T3433	punct	(,at
R2119	T3433	T3426	parataxis	at,earliest
R2120	T3434	T3433	pobj	E13.5,at
R2121	T3435	T3433	punct	),at
R2122	T3436	T3424	punct	", ",are
R2123	T3437	T3424	cc	and,are
R2124	T3438	T3439	dep	which,constitute
R2125	T3439	T3424	conj	constitute,are
R2126	T3440	T3441	amod	less,5
R2127	T3441	T3443	nummod	5,%
R2128	T3442	T3441	quantmod	than,5
R2129	T3443	T3439	dobj	%,constitute
R2130	T3444	T3443	prep	of,%
R2131	T3445	T3446	det	the,coat
R2132	T3446	T3444	pobj	coat,of
R2133	T3447	T3446	compound	mouse,coat
R2134	T3448	T3449	punct	(,data
R2135	T3449	T3414	meta	data,found
R2136	T3450	T3449	amod	unpublished,data
R2137	T3451	T3449	punct	),data
R2138	T3452	T3414	punct	.,found
R2139	T3454	T3455	mark	As,judged
R2140	T3455	T3456	advcl	judged,coincided
R2141	T3457	T3455	prep	by,judged
R2142	T3458	T3457	pobj	immunofluorescence,by
R2143	T3459	T3458	prep	with,immunofluorescence
R2144	T3460	T3459	pobj	antibodies,with
R2145	T3461	T3460	prep	against,antibodies
R2146	T3462	T3463	det	the,antigen
R2147	T3463	T3461	pobj	antigen,against
R2148	T3464	T3463	amod	proliferating,antigen
R2149	T3465	T3463	amod	nuclear,antigen
R2150	T3466	T3463	appos	Ki67,antigen
R2151	T3467	T3456	punct	", ",coincided
R2152	T3468	T3469	det	the,timing
R2153	T3469	T3456	nsubj	timing,coincided
R2154	T3470	T3469	prep	of,timing
R2155	T3471	T3472	compound	Snail,expression
R2156	T3472	T3470	pobj	expression,of
R2157	T3473	T3456	prep	with,coincided
R2158	T3474	T3475	det	the,stage
R2159	T3475	T3473	pobj	stage,with
R2160	T3476	T3477	prep	at,enhanced
R2161	T3477	T3475	relcl	enhanced,stage
R2162	T3478	T3476	pobj	which,at
R2163	T3479	T3480	det	the,follicle
R2164	T3480	T3477	nsubj	follicle,enhanced
R2165	T3481	T3480	amod	developing,follicle
R2166	T3482	T3483	poss	its,proliferation
R2167	T3483	T3477	dobj	proliferation,enhanced
R2168	T3484	T3483	cc	and,proliferation
R2169	T3485	T3486	amod	down,growth
R2170	T3486	T3483	conj	growth,proliferation
R2171	T3487	T3486	punct	-,growth
R2172	T3488	T3489	punct	(,1F
R2173	T3489	T3456	parataxis	1F,coincided
R2174	T3490	T3489	compound	Figure,1F
R2175	T3491	T3489	punct	),1F
R2176	T3492	T3456	punct	.,coincided
R2177	T3494	T3495	nsubj	Immunohistochemistry,marked
R2178	T3496	T3494	prep	with,Immunohistochemistry
R2179	T3497	T3496	pobj	antibodies,with
R2180	T3498	T3497	prep	against,antibodies
R2181	T3499	T3500	det	the,form
R2182	T3500	T3498	pobj	form,against
R2183	T3501	T3500	amod	active,form
R2184	T3502	T3500	punct	(,form
R2185	T3503	T3500	amod	phosphorylated,form
R2186	T3504	T3500	punct	),form
R2187	T3505	T3500	prep	of,form
R2188	T3506	T3505	pobj	MAPK,of
R2189	T3507	T3500	punct	(,form
R2190	T3508	T3500	appos	pMAPK,form
R2191	T3509	T3495	punct	),marked
R2192	T3510	T3511	det	a,subset
R2193	T3511	T3495	dobj	subset,marked
R2194	T3512	T3511	prep	of,subset
R2195	T3513	T3514	det	the,cells
R2196	T3514	T3512	pobj	cells,of
R2197	T3601	T3599	punct	;,1H
R2198	T3515	T3514	amod	proliferating,cells
R2199	T3602	T3599	advcl	see,1H
R2200	T3516	T3514	punct	(,cells
R2201	T3603	T3602	advmod	also,see
R2202	T3604	T3602	punct	[,see
R2203	T3517	T3518	npadvmod	Ki67,positive
R2204	T3605	T3602	dobj	4,see
R2205	T3606	T3599	punct	],1H
R2206	T3607	T3599	punct	),1H
R2207	T3518	T3514	amod	positive,cells
R2208	T3608	T3583	punct	.,exhibited
R2209	T3519	T3518	punct	-,positive
R2210	T3520	T3514	punct	),cells
R2211	T3521	T3495	punct	", ",marked
R2212	T3522	T3495	cc	and,marked
R2213	T3523	T3524	npadvmod	pMAPK,positive
R2214	T3524	T3526	amod	positive,cells
R2215	T3525	T3524	punct	-,positive
R2216	T3526	T3527	nsubjpass	cells,enriched
R2217	T3527	T3495	conj	enriched,marked
R2218	T3528	T3527	auxpass	were,enriched
R2219	T3529	T3527	prep	in,enriched
R2220	T3530	T3531	det	the,bud
R2221	T3531	T3529	pobj	bud,in
R2222	T3532	T3531	compound	hair,bud
R2223	T3533	T3534	punct	(,1G
R2224	T3534	T3527	parataxis	1G,enriched
R2225	T3535	T3534	compound	Figure,1G
R2226	T3536	T3534	punct	),1G
R2227	T3537	T3527	punct	.,enriched
R2228	T3539	T3540	det	The,timing
R2229	T3540	T3541	nsubj	timing,appeared
R2230	T3542	T3540	prep	of,timing
R2231	T3543	T3544	compound	Snail,induction
R2232	T3544	T3542	pobj	induction,of
R2233	T3545	T3544	cc	and,induction
R2234	T3546	T3547	nmod	Ki67,enrichment
R2235	T3547	T3544	conj	enrichment,induction
R2236	T3548	T3546	cc	and,Ki67
R2237	T3549	T3546	conj	pMAPK,Ki67
R2238	T3550	T3540	prep	in,timing
R2239	T3551	T3552	det	the,bud
R2240	T3552	T3550	pobj	bud,in
R2241	T3553	T3552	compound	hair,bud
R2242	T3554	T3555	aux	to,follow
R2243	T3555	T3541	xcomp	follow,appeared
R2244	T3556	T3555	advmod	closely,follow
R2245	T3557	T3558	det	the,induction
R2246	T3558	T3555	dobj	induction,follow
R2247	T3559	T3558	prep	of,induction
R2248	T3560	T3561	nmod	LEF,activity
R2249	T3561	T3559	pobj	activity,of
R2250	T3562	T3560	punct	-,LEF
R2251	T3563	T3560	nummod	1,LEF
R2252	T3564	T3560	punct	/,LEF
R2253	T3565	T3566	compound	β,catenin
R2254	T3566	T3560	appos	catenin,LEF
R2255	T3567	T3566	punct	-,catenin
R2256	T3568	T3561	punct	", ",activity
R2257	T3569	T3561	acl	known,activity
R2258	T3570	T3571	aux	to,initiate
R2259	T3571	T3569	xcomp	initiate,known
R2260	T3572	T3571	prep	in,initiate
R2261	T3573	T3574	det	the,stage
R2262	T3574	T3572	pobj	stage,in
R2263	T3575	T3576	compound	hair,placode
R2264	T3576	T3574	compound	placode,stage
R2265	T3577	T3578	punct	[,20
R2266	T3578	T3555	parataxis	20,follow
R2267	T3579	T3578	punct	],20
R2268	T3580	T3541	punct	.,appeared
R2269	T3582	T3583	advmod	However,exhibited
R227	T1019	T1020	amod	Mammalian,development
R2270	T3584	T3583	punct	", ",exhibited
R2271	T3585	T3583	prep	like,exhibited
R2272	T3586	T3585	pobj	placodes,like
R2273	T3587	T3583	punct	", ",exhibited
R2274	T3588	T3589	compound	hair,buds
R2275	T3589	T3583	nsubj	buds,exhibited
R2276	T3590	T3591	amod	down,regulation
R2277	T3591	T3583	dobj	regulation,exhibited
R2278	T3592	T3591	punct	-,regulation
R2279	T3593	T3591	prep	in,regulation
R228	T1020	T1021	nsubj	development,involves
R2280	T3594	T3595	compound	E,cadherin
R2281	T3595	T3597	compound	cadherin,expression
R2282	T3596	T3595	punct	-,cadherin
R2283	T3597	T3593	pobj	expression,in
R2284	T3598	T3599	punct	(,1H
R2285	T3599	T3583	parataxis	1H,exhibited
R2286	T3600	T3599	compound	Figure,1H
R2289	T3910	T3911	amod	Sustained,Expression
R229	T1022	T1023	det	the,morphogenesis
R2290	T3911	T3912	nsubj	Expression,Results
R2291	T3913	T3911	prep	of,Expression
R2292	T3914	T3913	pobj	Snail,of
R2293	T3915	T3912	prep	in,Results
R2294	T3916	T3917	amod	Epidermal,Hyperproliferation
R2295	T3917	T3915	pobj	Hyperproliferation,in
R2296	T3918	T3917	cc	and,Hyperproliferation
R2297	T3919	T3920	compound	Differentiation,Defects
R2298	T3920	T3917	conj	Defects,Hyperproliferation
R2299	T3921	T3912	prep	in,Results
R230	T1023	T1021	dobj	morphogenesis,involves
R2300	T3922	T3923	compound	Tg,Skin
R2301	T3923	T3921	pobj	Skin,in
R2302	T3924	T3923	compound	Mouse,Skin
R2303	T3926	T3927	det	The,spike
R2304	T3927	T3929	nsubj	spike,prompted
R2305	T3928	T3927	amod	striking,spike
R2306	T3930	T3927	prep	of,spike
R2307	T3931	T3932	compound	Snail,expression
R2308	T3932	T3930	pobj	expression,of
R2309	T3933	T3927	amod	coincident,spike
R231	T1024	T1023	prep	of,morphogenesis
R2310	T3934	T3933	prep	with,coincident
R2311	T3935	T3936	compound	hair,bud
R2312	T3936	T3937	compound	bud,formation
R2313	T3937	T3934	pobj	formation,with
R2314	T3938	T3937	cc	and,formation
R2315	T3939	T3940	amod	enhanced,proliferation
R2316	T3940	T3937	conj	proliferation,formation
R2317	T3941	T3929	dobj	us,prompted
R2318	T3942	T3943	aux	to,examine
R2319	T3943	T3929	xcomp	examine,prompted
R232	T1025	T1026	amod	complex,structures
R2320	T3944	T3945	det	the,consequences
R2321	T3945	T3943	dobj	consequences,examine
R2322	T3946	T3945	prep	of,consequences
R2323	T3947	T3948	advmod	ectopically,expressing
R2324	T3948	T3946	pcomp	expressing,of
R2325	T3949	T3948	dobj	Snail,expressing
R2326	T3950	T3948	advmod	elsewhere,expressing
R2327	T3951	T3950	prep	in,elsewhere
R2328	T3952	T3953	compound	mouse,epidermis
R2329	T3953	T3951	pobj	epidermis,in
R233	T1026	T1024	pobj	structures,of
R2330	T3954	T3953	compound	skin,epidermis
R2331	T3955	T3929	punct	.,prompted
R2332	T3957	T3958	aux	To,distinguish
R2333	T3958	T3959	advcl	distinguish,used
R2334	T3960	T3958	dobj	Tg,distinguish
R2335	T3961	T3958	prep	from,distinguish
R2336	T3962	T3963	amod	endogenous,Snail
R2337	T3963	T3961	pobj	Snail,from
R2338	T3964	T3959	punct	", ",used
R2339	T3965	T3959	nsubj	we,used
R234	T1027	T1028	advmod	three,dimensional
R2340	T3966	T3967	det	the,epitope
R2341	T3967	T3959	dobj	epitope,used
R2342	T3968	T3967	compound	HA,epitope
R2343	T3969	T3967	punct	-,epitope
R2344	T3970	T3967	punct	", ",epitope
R2345	T3971	T3967	acl	shown,epitope
R2346	T3972	T3971	advmod	previously,shown
R2347	T3973	T3974	neg	not,alter
R2348	T3974	T3971	xcomp	alter,shown
R2349	T3975	T3974	aux	to,alter
R235	T1028	T1026	amod	dimensional,structures
R2350	T3976	T3977	poss	Snail,activity
R2351	T3977	T3974	dobj	activity,alter
R2352	T3978	T3976	case	's,Snail
R2353	T3979	T3977	amod	transcriptional,activity
R2354	T3980	T3981	punct	[,12
R2355	T3981	T3959	parataxis	12,used
R2356	T3982	T3981	punct	],12
R2357	T3983	T3959	punct	.,used
R2358	T3985	T3986	prep	Of,expressed
R2359	T3987	T3988	nummod	20,animals
R236	T1029	T1028	punct	-,dimensional
R2360	T3988	T3985	pobj	animals,Of
R2361	T3989	T3990	nmod	K14,Snail
R2362	T3990	T3988	nmod	Snail,animals
R2363	T3991	T3990	punct	-,Snail
R2364	T3992	T3988	punct	[,animals
R2365	T3993	T3988	nmod	HA,animals
R2366	T3994	T3988	punct	],animals
R2367	T3995	T3988	compound	Tg,animals
R2368	T3996	T3988	acl	generated,animals
R2369	T3997	T3986	punct	", ",expressed
R237	T1030	T1023	prep	from,morphogenesis
R2370	T3998	T3986	nsubj	three,expressed
R2371	T3999	T4000	det	the,transgene
R2372	T4000	T3986	dobj	transgene,expressed
R2373	T4001	T3986	cc	and,expressed
R2374	T4002	T4003	nsubj	all,exhibited
R2375	T4003	T3986	conj	exhibited,expressed
R2376	T4004	T4005	amod	analogous,phenotypes
R2377	T4005	T4003	dobj	phenotypes,exhibited
R2378	T4006	T3986	punct	.,expressed
R2379	T4008	T4009	nsubj	Mice,died
R238	T1031	T1032	advmod	seemingly,uniform
R2380	T4010	T4011	dep	that,integrated
R2381	T4011	T4008	relcl	integrated,Mice
R2382	T4012	T4013	det	the,transgene
R2383	T4013	T4011	dobj	transgene,integrated
R2384	T4014	T4011	prep	at,integrated
R2385	T4015	T4016	det	the,stage
R2386	T4016	T4014	pobj	stage,at
R2387	T4017	T4018	amod	single,cell
R2388	T4018	T4016	compound	cell,stage
R2389	T4019	T4018	punct	-,cell
R239	T1032	T1033	amod	uniform,sheets
R2390	T4020	T4009	prep	at,died
R2391	T4021	T4020	cc	or,at
R2392	T4022	T4023	advmod	shortly,after
R2393	T4023	T4020	conj	after,at
R2394	T4024	T4023	pobj	birth,after
R2395	T4025	T4009	punct	.,died
R2396	T4027	T4028	det	The,mice
R2397	T4028	T4035	nsubj	mice,harbored
R2398	T4029	T4028	nummod	three,mice
R2399	T4030	T4028	amod	surviving,mice
R240	T1033	T1030	pobj	sheets,from
R2400	T4031	T4032	amod	full,Tg
R2401	T4032	T4028	compound	Tg,mice
R2402	T4033	T4032	punct	-,Tg
R2403	T4034	T4028	compound	founder,mice
R2404	T4036	T4037	compound	transgene,integrations
R2405	T4037	T4035	dobj	integrations,harbored
R2406	T4038	T4039	dep	that,gave
R2407	T4039	T4037	relcl	gave,integrations
R2408	T4040	T4041	amod	stable,transmission
R2409	T4041	T4039	dobj	transmission,gave
R241	T1034	T1033	cc	or,sheets
R2410	T4042	T4041	prep	of,transmission
R2411	T4043	T4044	amod	mosaic,gene
R2412	T4044	T4046	compound	gene,expression
R2413	T4045	T4044	compound	Snail,gene
R2414	T4046	T4042	pobj	expression,of
R2415	T4047	T4039	prep	through,gave
R2416	T4048	T4049	det	the,germline
R2417	T4049	T4047	pobj	germline,through
R2418	T4050	T4035	punct	.,harbored
R2419	T4052	T4053	advmod	Progressively,poor
R242	T1035	T1033	conj	masses,sheets
R2420	T4053	T4054	amod	poor,health
R2421	T4054	T4055	nsubj	health,necessitated
R2422	T4056	T4057	nsubj	our,sacrificing
R2423	T4057	T4055	ccomp	sacrificing,necessitated
R2424	T4058	T4059	amod	most,offspring
R2425	T4059	T4057	dobj	offspring,sacrificing
R2426	T4060	T4059	prep	from,offspring
R2427	T4061	T4062	det	these,lines
R2428	T4062	T4060	pobj	lines,from
R2429	T4063	T4057	prep	within,sacrificing
R243	T1036	T1033	prep	of,sheets
R2430	T4064	T4065	det	a,year
R2431	T4065	T4063	pobj	year,within
R2432	T4066	T4065	prep	of,year
R2433	T4067	T4066	pobj	birth,of
R2434	T4068	T4055	punct	.,necessitated
R2435	T4070	T4071	mark	As,grew
R2436	T4071	T4075	advcl	grew,became
R2437	T4072	T4073	compound	Snail,animals
R2438	T4073	T4071	nsubj	animals,grew
R2439	T4074	T4073	compound	Tg,animals
R244	T1037	T1036	pobj	cells,of
R2440	T4076	T4075	punct	", ",became
R2441	T4077	T4075	nsubj	they,became
R2442	T4078	T4075	acomp	distinguished,became
R2443	T4079	T4078	prep	by,distinguished
R2444	T4080	T4081	poss	their,size
R2445	T4081	T4079	pobj	size,by
R2446	T4082	T4081	amod	small,size
R2447	T4083	T4081	punct	", ",size
R2448	T4084	T4085	amod	short,tails
R2449	T4085	T4081	conj	tails,size
R245	T1038	T1021	punct	.,involves
R2450	T4086	T4085	punct	", ",tails
R2451	T4087	T4085	cc	and,tails
R2452	T4088	T4089	amod	flaky,skin
R2453	T4089	T4085	conj	skin,tails
R2454	T4090	T4091	punct	(,2A
R2455	T4091	T4075	parataxis	2A,became
R2456	T4092	T4091	compound	Figure,2A
R2457	T4093	T4091	punct	),2A
R2458	T4094	T4075	punct	.,became
R2459	T4096	T4097	amod	Histological,analyses
R246	T1040	T1041	det	A,structure
R2460	T4097	T4098	nsubj	analyses,revealed
R2461	T4099	T4097	prep	of,analyses
R2462	T4100	T4101	nummod	3,d
R2463	T4101	T4103	npadvmod	d,old
R2464	T4102	T4101	punct	-,d
R2465	T4103	T4104	amod	old,mice
R2466	T4104	T4099	pobj	mice,of
R2467	T4105	T4106	punct	(,P3
R2468	T4106	T4103	parataxis	P3,old
R2469	T4107	T4106	punct	),P3
R247	T1041	T1046	nsubj	structure,initiates
R2470	T4108	T4109	compound	mosaic,patches
R2471	T4109	T4098	dobj	patches,revealed
R2472	T4110	T4109	acl	marked,patches
R2473	T4111	T4110	agent	by,marked
R2474	T4112	T4113	amod	epidermal,thickening
R2475	T4113	T4111	pobj	thickening,by
R2476	T4114	T4115	punct	(,2B
R2477	T4115	T4098	parataxis	2B,revealed
R2478	T4116	T4115	compound	Figure,2B
R2479	T4117	T4115	punct	),2B
R248	T1042	T1041	amod	simple,structure
R2480	T4118	T4098	punct	.,revealed
R2481	T4120	T4121	det	The,morphology
R2482	T4121	T4123	nsubjpass	morphology,reflected
R2483	T4122	T4121	compound	mosaic,morphology
R2484	T4124	T4123	auxpass	was,reflected
R2485	T4125	T4123	prep	at,reflected
R2486	T4126	T4127	det	the,level
R2487	T4127	T4125	pobj	level,at
R2488	T4128	T4127	prep	of,level
R2489	T4129	T4130	compound	Tg,protein
R249	T1043	T1044	npadvmod	bud,like
R2490	T4130	T4128	pobj	protein,of
R2491	T4131	T4130	compound	Snail,protein
R2492	T4132	T4123	punct	", ",reflected
R2493	T4133	T4134	mark	with,expressing
R2494	T4134	T4123	advcl	expressing,reflected
R2495	T4135	T4136	advmod	only,regions
R2496	T4136	T4134	nsubj	regions,expressing
R2497	T4137	T4136	det	the,regions
R2498	T4138	T4136	amod	hyperthickened,regions
R2499	T4139	T4140	amod	nuclear,Snail
R250	T1044	T1041	amod	like,structure
R2500	T4140	T4134	dobj	Snail,expressing
R2501	T4141	T4142	npadvmod	HA,tagged
R2502	T4142	T4140	amod	tagged,Snail
R2503	T4143	T4142	punct	-,tagged
R2504	T4144	T4145	punct	(,2C
R2505	T4145	T4123	parataxis	2C,reflected
R2506	T4146	T4145	compound	Figure,2C
R2507	T4147	T4145	punct	),2C
R2508	T4148	T4123	punct	.,reflected
R2509	T4150	T4151	det	These,areas
R251	T1045	T1044	punct	-,like
R2510	T4151	T4153	nsubjpass	areas,marked
R2511	T4152	T4151	amod	hyperthickened,areas
R2512	T4154	T4153	auxpass	were,marked
R2513	T4155	T4153	agent	by,marked
R2514	T4156	T4157	amod	excessive,proliferation
R2515	T4157	T4155	pobj	proliferation,by
R2516	T4158	T4153	punct	", ",marked
R2517	T4159	T4160	mark	as,revealed
R2518	T4160	T4153	advcl	revealed,marked
R2519	T4161	T4160	agent	by,revealed
R252	T1047	T1048	det	the,formation
R2520	T4162	T4161	pobj	antibodies,by
R2521	T4163	T4162	prep	against,antibodies
R2522	T4164	T4165	det	the,antigen
R2523	T4165	T4163	pobj	antigen,against
R2524	T4166	T4165	amod	proliferating,antigen
R2525	T4167	T4165	amod	nuclear,antigen
R2526	T4168	T4165	appos	Ki67,antigen
R2527	T4169	T4170	punct	(,2D
R2528	T4170	T4153	parataxis	2D,marked
R2529	T4171	T4170	compound	Figure,2D
R253	T1048	T1046	dobj	formation,initiates
R2530	T4172	T4170	cc	and,2D
R2531	T4173	T4170	conj	2E,2D
R2532	T4174	T4170	punct	),2D
R2533	T4175	T4153	punct	.,marked
R2534	T4177	T4178	amod	Activated,cells
R2535	T4178	T4183	nsubj	cells,were
R2536	T4179	T4178	punct	", ",cells
R2537	T4180	T4181	npadvmod	pMAPK,positive
R2538	T4181	T4178	amod	positive,cells
R2539	T4182	T4181	punct	-,positive
R254	T1049	T1048	prep	of,formation
R2540	T4184	T4183	advmod	also,were
R2541	T4185	T4183	acomp	prevalent,were
R2542	T4186	T4183	prep	in,were
R2543	T4187	T4188	det	these,areas
R2544	T4188	T4186	pobj	areas,in
R2545	T4189	T4190	punct	(,2F
R2546	T4190	T4183	parataxis	2F,were
R2547	T4191	T4190	compound	Figure,2F
R2548	T4192	T4190	cc	and,2F
R2549	T4193	T4190	conj	2G,2F
R255	T1050	T1051	amod	many,organs
R2550	T4194	T4190	punct	),2F
R2551	T4195	T4183	punct	", ",were
R2552	T4196	T4197	mark	as,were
R2553	T4197	T4183	advcl	were,were
R2554	T4198	T4197	nsubj	cells,were
R2555	T4199	T4198	acl	expressing,cells
R2556	T4200	T4199	dobj	keratin,expressing
R2557	T4201	T4200	nummod	6,keratin
R2558	T4202	T4200	punct	", ",keratin
R2559	T4203	T4204	det	a,keratin
R256	T1051	T1049	pobj	organs,of
R2560	T4204	T4200	appos	keratin,keratin
R2561	T4205	T4204	acl	induced,keratin
R2562	T4206	T4205	prep	in,induced
R2563	T4207	T4208	det	the,layers
R2564	T4208	T4206	pobj	layers,in
R2565	T4209	T4208	amod	suprabasal,layers
R2566	T4210	T4208	prep	of,layers
R2567	T4211	T4212	amod	hyperproliferative,skin
R2568	T4212	T4210	pobj	skin,of
R2569	T4213	T4214	punct	(,2H
R257	T1052	T1051	punct	", ",organs
R2570	T4214	T4183	parataxis	2H,were
R2571	T4215	T4214	compound	Figure,2H
R2572	T4216	T4214	cc	and,2H
R2573	T4217	T4214	conj	2I,2H
R2574	T4218	T4214	punct	),2H
R2575	T4219	T4183	punct	.,were
R2576	T4221	T4222	nsubj	Expression,block
R2577	T4223	T4221	prep	of,Expression
R2578	T4224	T4225	det	the,transgene
R2579	T4225	T4223	pobj	transgene,of
R258	T1053	T1051	prep	including,organs
R2580	T4226	T4225	compound	Snail,transgene
R2581	T4227	T4222	aux	did,block
R2582	T4228	T4222	neg	not,block
R2583	T4229	T4230	amod	terminal,differentiation
R2584	T4230	T4222	dobj	differentiation,block
R2585	T4231	T4222	prep	in,block
R2586	T4232	T4233	det	the,epidermis
R2587	T4233	T4231	pobj	epidermis,in
R2588	T4234	T4233	amod	hyperproliferative,epidermis
R2589	T4235	T4222	punct	", ",block
R259	T1054	T1053	pobj	lungs,including
R2590	T4236	T4222	cc	but,block
R2591	T4237	T4238	nsubj	it,distorted
R2592	T4238	T4222	conj	distorted,block
R2593	T4239	T4238	dobj	it,distorted
R2594	T4240	T4238	advmod	markedly,distorted
R2595	T4241	T4242	punct	(,3A
R2596	T4242	T4238	parataxis	3A,distorted
R2597	T4243	T4242	compound	Figure,3A
R2598	T4244	T4245	punct	–,3H
R2599	T4245	T4242	prep	3H,3A
R260	T1055	T1054	punct	", ",lungs
R2600	T4246	T4242	punct	),3A
R2601	T4247	T4238	punct	.,distorted
R2602	T4249	T4250	advcl	Typical,led
R2603	T4251	T4249	prep	of,Typical
R2604	T4252	T4253	amod	most,conditions
R2605	T4253	T4251	pobj	conditions,of
R2606	T4254	T4253	compound	hyperproliferating,conditions
R2607	T4255	T4250	punct	", ",led
R2608	T4256	T4257	compound	Snail,expression
R2609	T4257	T4250	nsubj	expression,led
R261	T1056	T1057	amod	spinal,cord
R2610	T4258	T4250	prep	to,led
R2611	T4259	T4260	det	a,expansion
R2612	T4260	T4258	pobj	expansion,to
R2613	T4261	T4260	amod	large,expansion
R2614	T4262	T4250	prep	in,led
R2615	T4263	T4262	pobj	layers,in
R2616	T4264	T4263	prep	with,layers
R2617	T4265	T4266	amod	spinous,morphology
R2618	T4266	T4264	pobj	morphology,with
R2619	T4267	T4265	cc	and,spinous
R262	T1057	T1054	conj	cord,lungs
R2620	T4268	T4265	conj	granular,spinous
R2621	T4269	T4250	punct	.,led
R2622	T4271	T4272	advmod	Additionally,was
R2623	T4273	T4272	punct	", ",was
R2624	T4274	T4272	advmod	however,was
R2625	T4275	T4272	punct	", ",was
R2626	T4276	T4277	det	a,expansion
R2627	T4277	T4272	attr	expansion,was
R2628	T4278	T4277	amod	marked,expansion
R2629	T4279	T4278	cc	and,marked
R263	T1058	T1057	punct	", ",cord
R2630	T4280	T4278	conj	variable,marked
R2631	T4281	T4277	prep	of,expansion
R2632	T4282	T4281	pobj	keratin,of
R2633	T4283	T4282	nummod	5,keratin
R2634	T4284	T4282	punct	(,keratin
R2635	T4285	T4282	appos	K5,keratin
R2636	T4286	T4282	punct	),keratin
R2637	T4287	T4282	punct	", ",keratin
R2638	T4288	T4289	advmod	normally,restricted
R2639	T4289	T4282	acl	restricted,keratin
R264	T1059	T1060	amod	mammary,glands
R2640	T4290	T4289	prep	to,restricted
R2641	T4291	T4292	det	the,layer
R2642	T4292	T4290	pobj	layer,to
R2643	T4293	T4292	amod	innermost,layer
R2644	T4294	T4292	amod	basal,layer
R2645	T4295	T4296	punct	(,see
R2646	T4296	T4272	parataxis	see,was
R2647	T4297	T4296	dobj	Figure,see
R2648	T4298	T4297	nummod	3,Figure
R2649	T4299	T4296	punct	),see
R265	T1060	T1057	conj	glands,cord
R2650	T4300	T4272	punct	.,was
R2651	T4302	T4303	mark	Although,precluded
R2652	T4303	T4318	advcl	precluded,emerged
R2653	T4304	T4305	det	the,failure
R2654	T4305	T4303	nsubj	failure,precluded
R2655	T4306	T4305	prep	of,failure
R2656	T4307	T4308	nmod	Snail,mice
R2657	T4308	T4306	pobj	mice,of
R2658	T4309	T4307	punct	-,Snail
R2659	T4310	T4307	amod	null,Snail
R266	T1061	T1060	punct	", ",glands
R2660	T4311	T4312	aux	to,develop
R2661	T4312	T4305	acl	develop,failure
R2662	T4313	T4314	amod	past,gastrulation
R2663	T4314	T4312	dobj	gastrulation,develop
R2664	T4315	T4316	punct	[,21
R2665	T4316	T4305	parataxis	21,failure
R2666	T4317	T4316	punct	],21
R2667	T4319	T4320	poss	our,ability
R2668	T4320	T4303	dobj	ability,precluded
R2669	T4321	T4322	aux	to,study
R267	T1062	T1060	cc	and,glands
R2670	T4322	T4320	acl	study,ability
R2671	T4323	T4324	det	the,loss
R2672	T4324	T4322	dobj	loss,study
R2673	T4325	T4324	prep	of,loss
R2674	T4326	T4327	compound	Snail,function
R2675	T4327	T4325	pobj	function,of
R2676	T4328	T4324	prep	in,loss
R2677	T4329	T4330	compound	skin,development
R2678	T4330	T4328	pobj	development,in
R2679	T4331	T4318	punct	", ",emerged
R268	T1063	T1064	compound	hair,follicles
R2680	T4332	T4333	det	a,correlation
R2681	T4333	T4318	nsubj	correlation,emerged
R2682	T4334	T4333	amod	good,correlation
R2683	T4335	T4318	prep	between,emerged
R2684	T4336	T4337	det	the,expression
R2685	T4337	T4335	pobj	expression,between
R2686	T4338	T4337	prep	of,expression
R2687	T4339	T4340	compound	Snail,protein
R2688	T4340	T4338	pobj	protein,of
R2689	T4341	T4337	cc	and,expression
R269	T1064	T1060	conj	follicles,glands
R2690	T4342	T4343	det	the,extension
R2691	T4343	T4337	conj	extension,expression
R2692	T4344	T4343	prep	of,extension
R2693	T4345	T4344	pobj	K5,of
R2694	T4346	T4345	punct	", ",K5
R2695	T4347	T4345	conj	Ki67,K5
R2696	T4348	T4347	punct	", ",Ki67
R2697	T4349	T4347	cc	and,Ki67
R2698	T4350	T4347	conj	pMAPK,Ki67
R2699	T4351	T4343	advmod	suprabasally,extension
R270	T1065	T1066	punct	[,1
R2700	T4352	T4353	punct	(,compare
R2701	T4353	T4318	parataxis	compare,emerged
R2702	T4354	T4353	dobj	data,compare
R2703	T4355	T4353	prep	in,compare
R2704	T4356	T4357	nmod	Figures,2
R2705	T4357	T4355	pobj	2,in
R2706	T4358	T4357	cc	and,2
R2707	T4359	T4357	conj	3,2
R2708	T4360	T4353	punct	),compare
R2709	T4361	T4318	punct	.,emerged
R271	T1066	T1046	parataxis	1,initiates
R2710	T4363	T4364	det	The,changes
R2711	T4364	T4365	nsubjpass	changes,accompanied
R2712	T4366	T4364	prep	in,changes
R2713	T4367	T4366	pobj	hyperproliferation,in
R2714	T4368	T4367	cc	and,hyperproliferation
R2715	T4369	T4367	conj	differentiation,hyperproliferation
R2716	T4370	T4365	auxpass	were,accompanied
R2717	T4371	T4365	neg	not,accompanied
R2718	T4372	T4365	advmod	initially,accompanied
R2719	T4373	T4365	agent	by,accompanied
R272	T1067	T1066	punct	],1
R2720	T4374	T4375	amod	gross,signs
R2721	T4375	T4373	pobj	signs,by
R2722	T4376	T4375	prep	of,signs
R2723	T4377	T4378	amod	epithelial,invaginations
R2724	T4378	T4376	pobj	invaginations,of
R2725	T4379	T4365	punct	.,accompanied
R2726	T4381	T4382	prep	With,developed
R2727	T4383	T4381	pobj	age,With
R2728	T4384	T4382	punct	", ",developed
R2729	T4385	T4382	advmod	however,developed
R273	T1068	T1046	punct	.,initiates
R2730	T4386	T4382	punct	", ",developed
R2731	T4387	T4388	amod	epidermal,folds
R2732	T4388	T4382	nsubj	folds,developed
R2733	T4389	T4388	cc	and,folds
R2734	T4390	T4388	conj	undulations,folds
R2735	T4391	T4382	prep	in,developed
R2736	T4392	T4391	pobj	areas,in
R2737	T4393	T4394	advmod	where,expressed
R2738	T4394	T4392	relcl	expressed,areas
R2739	T4395	T4394	nsubjpass	Snail,expressed
R274	T1070	T1071	det	The,cells
R2740	T4396	T4394	auxpass	was,expressed
R2741	T4397	T4382	punct	", ",developed
R2742	T4398	T4382	cc	and,developed
R2743	T4399	T4400	amod	proliferative,markers
R2744	T4400	T4401	nsubj	markers,persisted
R2745	T4401	T4382	conj	persisted,developed
R2746	T4402	T4401	prep	in,persisted
R2747	T4403	T4404	det	these,regions
R2748	T4404	T4402	pobj	regions,in
R2749	T4405	T4406	punct	(,staining
R275	T1071	T1076	nsubjpass	cells,attached
R2750	T4406	T4401	parataxis	staining,persisted
R2751	T4407	T4408	compound	Figure,3I
R2752	T4408	T4406	dep	3I,staining
R2753	T4409	T4408	cc	and,3I
R2754	T4410	T4408	conj	3J,3I
R2755	T4411	T4406	punct	;,staining
R2756	T4412	T4413	amod	anti-cyclin,D
R2757	T4413	T4406	compound	D,staining
R2758	T4414	T4406	punct	),staining
R2759	T4415	T4382	punct	.,developed
R276	T1072	T1071	amod	multipotent,cells
R2760	T4417	T4418	det	The,undulations
R2761	T4418	T4419	nsubjpass	undulations,accompanied
R2762	T4420	T4419	auxpass	were,accompanied
R2763	T4421	T4419	agent	by,accompanied
R2764	T4422	T4423	amod	partial,dissolution
R2765	T4423	T4421	pobj	dissolution,by
R2766	T4424	T4423	prep	of,dissolution
R2767	T4425	T4426	det	the,membrane
R2768	T4426	T4424	pobj	membrane,of
R2769	T4427	T4426	amod	underlying,membrane
R277	T1073	T1071	punct	", ",cells
R2770	T4428	T4426	compound	basement,membrane
R2771	T4429	T4430	punct	(,3K
R2772	T4430	T4419	parataxis	3K,accompanied
R2773	T4431	T4430	compound	Figure,3K
R2774	T4432	T4430	cc	and,3K
R2775	T4433	T4430	conj	3L,3K
R2776	T4434	T4430	punct	),3K
R2777	T4435	T4419	punct	.,accompanied
R2778	T4437	T4438	amod	Aberrant,staining
R2779	T4438	T4439	nsubjpass	staining,observed
R278	T1074	T1071	amod	adhering,cells
R2780	T4440	T4439	auxpass	was,observed
R2781	T4441	T4439	advmod	also,observed
R2782	T4442	T4439	prep	with,observed
R2783	T4443	T4442	pobj	antibodies,with
R2784	T4444	T4443	prep	against,antibodies
R2785	T4445	T4444	pobj	components,against
R2786	T4446	T4445	prep	of,components
R2787	T4447	T4448	det	the,hemidesmosomes
R2788	T4448	T4446	pobj	hemidesmosomes,of
R2789	T4449	T4445	punct	", ",components
R279	T1075	T1071	amod	epithelial,cells
R2790	T4450	T4451	dep	which,provide
R2791	T4451	T4445	relcl	provide,components
R2792	T4452	T4453	amod	strong,adhesion
R2793	T4453	T4451	dobj	adhesion,provide
R2794	T4454	T4453	prep	of,adhesion
R2795	T4455	T4456	amod	basal,cells
R2796	T4456	T4454	pobj	cells,of
R2797	T4457	T4456	amod	epidermal,cells
R2798	T4458	T4451	prep	to,provide
R2799	T4459	T4460	det	the,lamina
R280	T1077	T1076	auxpass	are,attached
R2800	T4460	T4458	pobj	lamina,to
R2801	T4461	T4460	amod	underlying,lamina
R2802	T4462	T4460	amod	basal,lamina
R2803	T4463	T4464	punct	(,3M
R2804	T4464	T4439	parataxis	3M,observed
R2805	T4465	T4464	compound	Figure,3M
R2806	T4466	T4464	cc	and,3M
R2807	T4467	T4464	conj	3N,3M
R2808	T4468	T4464	punct	),3M
R2809	T4469	T4439	punct	.,observed
R281	T1078	T1076	advmod	typically,attached
R2810	T4471	T4472	advmod	Interestingly,occur
R2811	T4473	T4472	punct	", ",occur
R2812	T4474	T4475	amod	similar,types
R2813	T4475	T4472	nsubj	types,occur
R2814	T4476	T4475	prep	of,types
R2815	T4477	T4476	pobj	alterations,of
R2816	T4478	T4472	prep	in,occur
R2817	T4479	T4480	det	the,membrane
R2818	T4480	T4478	pobj	membrane,in
R2819	T4481	T4480	compound	basement,membrane
R282	T1079	T1076	prep	to,attached
R2820	T4482	T4472	prep	in,occur
R2821	T4483	T4484	det	the,bud
R2822	T4484	T4482	pobj	bud,in
R2823	T4485	T4484	compound	hair,bud
R2824	T4486	T4484	prep	of,bud
R2825	T4487	T4488	amod	embryonic,mice
R2826	T4488	T4486	pobj	mice,of
R2827	T4489	T4487	cc	and,embryonic
R2828	T4490	T4487	conj	newborn,embryonic
R2829	T4491	T4492	advmod	when,expressed
R283	T1080	T1081	det	an,lamina
R2830	T4492	T4472	advcl	expressed,occur
R2831	T4493	T4492	nsubjpass	Snail,expressed
R2832	T4494	T4492	auxpass	is,expressed
R2833	T4495	T4492	advmod	normally,expressed
R2834	T4496	T4472	punct	.,occur
R2835	T4498	T4499	det	The,fact
R2836	T4499	T4500	nsubj	fact,suggests
R2837	T4501	T4502	mark	that,altered
R2838	T4502	T4499	acl	altered,fact
R2839	T4503	T4504	det	the,membrane
R284	T1081	T1079	pobj	lamina,to
R2840	T4504	T4502	nsubjpass	membrane,altered
R2841	T4505	T4504	compound	basement,membrane
R2842	T4506	T4504	acl	separating,membrane
R2843	T4507	T4508	det	the,epidermis
R2844	T4508	T4506	dobj	epidermis,separating
R2845	T4509	T4506	prep	from,separating
R2846	T4510	T4511	det	the,dermis
R2847	T4511	T4509	pobj	dermis,from
R2848	T4512	T4502	auxpass	is,altered
R2849	T4513	T4514	advmod	only,in
R285	T1082	T1081	amod	underlying,lamina
R2850	T4514	T4502	prep	in,altered
R2851	T4515	T4516	det	the,animals
R2852	T4516	T4514	pobj	animals,in
R2853	T4517	T4516	amod	adult,animals
R2854	T4518	T4516	compound	Tg,animals
R2855	T4519	T4520	det	the,involvement
R2856	T4520	T4500	dobj	involvement,suggests
R2857	T4521	T4520	prep	of,involvement
R2858	T4522	T4523	amod	intermediary,factors
R2859	T4523	T4521	pobj	factors,of
R286	T1083	T1081	amod	basal,lamina
R2860	T4524	T4525	neg	not,available
R2861	T4525	T4523	relcl	available,factors
R2862	T4526	T4527	advmod	as,readily
R2863	T4527	T4525	advmod	readily,available
R2864	T4528	T4525	prep	in,available
R2865	T4529	T4530	det	the,epidermis
R2866	T4530	T4528	pobj	epidermis,in
R2867	T4531	T4532	mark	as,are
R2868	T4532	T4525	advcl	are,available
R2869	T4533	T4532	nsubj	they,are
R287	T1084	T1085	dep	that,polarizes
R2870	T4534	T4532	prep	in,are
R2871	T4535	T4536	det	the,follicle
R2872	T4536	T4534	pobj	follicle,in
R2873	T4537	T4500	punct	.,suggests
R2878	T5218	T5219	amod	Possible,Links
R2879	T5220	T5219	prep	between,Links
R288	T1085	T1081	relcl	polarizes,lamina
R2880	T5221	T5222	amod	Epidermal,Hyperproliferation
R2881	T5222	T5220	pobj	Hyperproliferation,between
R2882	T5223	T5222	cc	and,Hyperproliferation
R2883	T5224	T5225	amod	Down,regulation
R2884	T5225	T5222	conj	regulation,Hyperproliferation
R2885	T5226	T5225	punct	-,regulation
R2886	T5227	T5225	prep	of,regulation
R2887	T5228	T5229	compound	AJ,Proteins
R2888	T5229	T5227	pobj	Proteins,of
R2889	T5230	T5219	prep	in,Links
R289	T1086	T1087	det	the,sheet
R2890	T5231	T5232	compound	Snail,Mice
R2891	T5232	T5230	pobj	Mice,in
R2892	T5233	T5232	compound	Tg,Mice
R2893	T5235	T5236	prep	Given,examined
R2894	T5237	T5238	mark	that,is
R2895	T5238	T5235	pcomp	is,Given
R2896	T5239	T5240	det	the,promoter
R2897	T5240	T5238	nsubj	promoter,is
R2898	T5241	T5242	compound	E,cadherin
R2899	T5242	T5240	compound	cadherin,promoter
R290	T1087	T1085	dobj	sheet,polarizes
R2900	T5243	T5242	punct	-,cadherin
R2901	T5244	T5245	det	a,target
R2902	T5245	T5238	attr	target,is
R2903	T5246	T5245	amod	direct,target
R2904	T5247	T5245	prep	for,target
R2905	T5248	T5249	npadvmod	Snail,mediated
R2906	T5249	T5251	amod	mediated,repression
R2907	T5250	T5249	punct	-,mediated
R2908	T5251	T5247	pobj	repression,for
R2909	T5252	T5253	advmod	in,vitro
R291	T1088	T1087	amod	epithelial,sheet
R2910	T5253	T5238	advmod	vitro,is
R2911	T5254	T5255	punct	[,13
R2912	T5255	T5238	parataxis	13,is
R2913	T5256	T5255	nummod	4,13
R2914	T5257	T5255	punct	",",13
R2915	T5258	T5255	nummod	12,13
R2916	T5259	T5255	punct	",",13
R2917	T5260	T5255	punct	],13
R2918	T5261	T5238	punct	", ",is
R2919	T5262	T5238	cc	and,is
R292	T1089	T1085	cc	and,polarizes
R2920	T5263	T5264	mark	that,regulated
R2921	T5264	T5238	conj	regulated,is
R2922	T5265	T5266	compound	E,cadherin
R2923	T5266	T5264	nsubjpass	cadherin,regulated
R2924	T5267	T5266	punct	-,cadherin
R2925	T5268	T5264	auxpass	was,regulated
R2926	T5269	T5264	advmod	down,regulated
R2927	T5270	T5264	punct	-,regulated
R2928	T5271	T5264	prep	in,regulated
R2929	T5272	T5273	npadvmod	Snail,expressing
R293	T1090	T1085	conj	separates,polarizes
R2930	T5273	T5275	amod	expressing,buds
R2931	T5274	T5273	punct	-,expressing
R2932	T5275	T5271	pobj	buds,in
R2933	T5276	T5275	compound	hair,buds
R2934	T5277	T5236	punct	", ",examined
R2935	T5278	T5236	nsubj	we,examined
R2936	T5279	T5280	det	the,status
R2937	T5280	T5236	dobj	status,examined
R2938	T5281	T5280	prep	of,status
R2939	T5282	T5283	compound	E,cadherin
R294	T1091	T1090	dobj	it,separates
R2940	T5283	T5281	pobj	cadherin,of
R2941	T5284	T5283	punct	-,cadherin
R2942	T5285	T5283	cc	and,cadherin
R2943	T5286	T5287	amod	other,proteins
R2944	T5287	T5283	conj	proteins,cadherin
R2945	T5288	T5287	compound	AJ,proteins
R2946	T5289	T5236	prep	within,examined
R2947	T5290	T5289	pobj	regions,within
R2948	T5291	T5290	prep	of,regions
R2949	T5292	T5293	amod	hyperproliferative,epidermis
R295	T1092	T1090	prep	from,separates
R2950	T5293	T5291	pobj	epidermis,of
R2951	T5294	T5295	advmod	where,was
R2952	T5295	T5290	relcl	was,regions
R2953	T5296	T5297	compound	Tg,Snail
R2954	T5297	T5295	nsubj	Snail,was
R2955	T5298	T5295	acomp	present,was
R2956	T5299	T5300	punct	(,4A
R2957	T5300	T5236	parataxis	4A,examined
R2958	T5301	T5300	compound	Figure,4A
R2959	T5302	T5300	punct	),4A
R296	T1093	T1094	amod	surrounding,mesenchyme
R2960	T5303	T5236	punct	.,examined
R2961	T5305	T5306	prep	In,diminished
R2962	T5307	T5308	det	these,regions
R2963	T5308	T5305	pobj	regions,In
R2964	T5309	T5306	punct	", ",diminished
R2965	T5310	T5311	compound	immunofluorescence,staining
R2966	T5311	T5306	nsubjpass	staining,diminished
R2967	T5312	T5311	prep	of,staining
R2968	T5313	T5314	compound	E,cadherin
R2969	T5314	T5312	pobj	cadherin,of
R297	T1094	T1092	pobj	mesenchyme,from
R2970	T5315	T5314	punct	-,cadherin
R2971	T5316	T5314	cc	and,cadherin
R2972	T5317	T5318	compound	α,catenin
R2973	T5318	T5314	conj	catenin,cadherin
R2974	T5319	T5318	punct	-,catenin
R2975	T5320	T5306	auxpass	were,diminished
R2976	T5321	T5306	advmod	markedly,diminished
R2977	T5322	T5306	punct	.,diminished
R2978	T5324	T5325	prep	In,was
R2979	T5326	T5324	pobj	contrast,In
R298	T1095	T1076	punct	.,attached
R2980	T5327	T5325	punct	", ",was
R2981	T5328	T5329	det	the,intensity
R2982	T5329	T5325	nsubj	intensity,was
R2983	T5330	T5329	prep	of,intensity
R2984	T5331	T5332	compound	antibody,staining
R2985	T5332	T5330	pobj	staining,of
R2986	T5333	T5329	prep	for,intensity
R2987	T5334	T5335	nummod	two,proteins
R2988	T5335	T5333	pobj	proteins,for
R2989	T5336	T5335	amod	other,proteins
R299	T1097	T1098	amod	Budding,morphogenesis
R2990	T5337	T5335	compound	AJ,proteins
R2991	T5338	T5335	punct	", ",proteins
R2992	T5339	T5340	compound	β,catenin
R2993	T5340	T5335	appos	catenin,proteins
R2994	T5341	T5340	punct	-,catenin
R2995	T5342	T5340	cc	and,catenin
R2996	T5343	T5340	conj	Ajuba,catenin
R2997	T5344	T5325	punct	", ",was
R2998	T5345	T5325	advmod	still,was
R2999	T5346	T5325	acomp	strong,was
R300	T1098	T1099	nsubjpass	morphogenesis,guided
R3000	T5347	T5325	punct	.,was
R3001	T5349	T5350	advmod	Interestingly,appeared
R3002	T5351	T5350	punct	", ",appeared
R3003	T5352	T5350	advmod	however,appeared
R3004	T5353	T5350	punct	", ",appeared
R3005	T5354	T5350	prep	despite,appeared
R3006	T5355	T5356	amod	appreciable,immunofluorescence
R3007	T5356	T5354	pobj	immunofluorescence,despite
R3008	T5357	T5350	punct	", ",appeared
R3009	T5358	T5350	nsubj	localization,appeared
R301	T1100	T1099	auxpass	is,guided
R3010	T5359	T5358	prep	of,localization
R3011	T5360	T5361	compound	β,catenin
R3012	T5361	T5359	pobj	catenin,of
R3013	T5362	T5361	punct	-,catenin
R3014	T5363	T5361	cc	and,catenin
R3015	T5364	T5361	conj	Ajuba,catenin
R3016	T5365	T5366	aux	to,be
R3017	T5366	T5350	xcomp	be,appeared
R3018	T5367	T5368	advmod	largely,cytoplasmic
R3019	T5368	T5366	acomp	cytoplasmic,be
R302	T1101	T1099	agent	by,guided
R3020	T5369	T5370	advmod	rather,than
R3021	T5370	T5368	cc	than,cytoplasmic
R3022	T5371	T5368	conj	at,cytoplasmic
R3023	T5372	T5373	compound	cell,cell
R3024	T5373	T5375	compound	cell,borders
R3025	T5374	T5373	punct	-,cell
R3026	T5375	T5371	pobj	borders,at
R3027	T5376	T5377	punct	(,insets
R3028	T5377	T5350	parataxis	insets,appeared
R3029	T5378	T5379	compound	Figure,4A
R303	T1102	T1103	det	a,exchange
R3030	T5379	T5377	compound	4A,insets
R3031	T5380	T5377	punct	),insets
R3032	T5381	T5350	punct	.,appeared
R3033	T5383	T5384	amod	Architectural,differences
R3034	T5384	T5385	nsubj	differences,made
R3035	T5386	T5384	prep	in,differences
R3036	T5387	T5388	det	the,epidermis
R3037	T5388	T5386	pobj	epidermis,in
R3038	T5389	T5390	compound	Western,blot
R3039	T5390	T5391	compound	blot,analyses
R304	T1103	T1101	pobj	exchange,by
R3040	T5391	T5392	nsubj	analyses,difficult
R3041	T5392	T5385	ccomp	difficult,made
R3042	T5393	T5392	advmod	somewhat,difficult
R3043	T5394	T5395	aux	to,gauge
R3044	T5395	T5392	advcl	gauge,difficult
R3045	T5396	T5385	punct	.,made
R3046	T5398	T5399	advmod	However,accentuated
R3047	T5400	T5399	punct	", ",accentuated
R3048	T5401	T5399	prep	in,accentuated
R3049	T5402	T5401	pobj	regions,in
R305	T1104	T1103	amod	reciprocal,exchange
R3050	T5403	T5404	amod	such,as
R3051	T5404	T5402	prep	as,regions
R3052	T5405	T5406	compound	ear,skin
R3053	T5406	T5404	pobj	skin,as
R3054	T5407	T5402	punct	", ",regions
R3055	T5408	T5409	advmod	where,expressed
R3056	T5409	T5402	relcl	expressed,regions
R3057	T5410	T5411	det	the,levels
R3058	T5411	T5409	nsubjpass	levels,expressed
R3059	T5412	T5411	amod	highest,levels
R306	T1105	T1103	prep	of,exchange
R3060	T5413	T5411	prep	of,levels
R3061	T5414	T5415	compound	Snail,protein
R3062	T5415	T5413	pobj	protein,of
R3063	T5416	T5409	auxpass	were,expressed
R3064	T5417	T5399	punct	", ",accentuated
R3065	T5418	T5419	det	the,effects
R3066	T5419	T5399	nsubjpass	effects,accentuated
R3067	T5420	T5399	auxpass	were,accentuated
R3068	T5421	T5399	punct	.,accentuated
R3069	T5423	T5424	prep	In,reduced
R307	T1106	T1105	pobj	signals,of
R3070	T5425	T5426	preconj	both,skin
R3071	T5426	T5423	pobj	skin,In
R3072	T5427	T5426	compound	back,skin
R3073	T5428	T5426	cc	and,skin
R3074	T5429	T5430	compound	ear,skin
R3075	T5430	T5426	conj	skin,skin
R3076	T5431	T5424	punct	", ",reduced
R3077	T5432	T5433	amod	overall,levels
R3078	T5433	T5424	nsubjpass	levels,reduced
R3079	T5434	T5433	prep	of,levels
R308	T1107	T1103	prep	between,exchange
R3080	T5435	T5436	compound	E,cadherin
R3081	T5436	T5434	pobj	cadherin,of
R3082	T5437	T5436	punct	-,cadherin
R3083	T5438	T5436	cc	and,cadherin
R3084	T5439	T5440	compound	α,catenin
R3085	T5440	T5436	conj	catenin,cadherin
R3086	T5441	T5440	punct	-,catenin
R3087	T5442	T5424	auxpass	were,reduced
R3088	T5443	T5424	punct	", ",reduced
R3089	T5444	T5424	prep	under,reduced
R309	T1108	T1107	pobj	epithelium,between
R3090	T5445	T5444	pobj	conditions,under
R3091	T5446	T5447	advmod	where,remained
R3092	T5447	T5445	relcl	remained,conditions
R3093	T5448	T5449	nmod	β,catenin
R3094	T5449	T5451	nmod	catenin,levels
R3095	T5450	T5449	punct	-,catenin
R3096	T5451	T5447	nsubj	levels,remained
R3097	T5452	T5449	cc	and,catenin
R3098	T5453	T5449	conj	Ajuba,catenin
R3099	T5454	T5447	acomp	unchanged,remained
R310	T1109	T1108	cc	and,epithelium
R3100	T5455	T5447	advcl	relative,remained
R3101	T5456	T5455	prep	to,relative
R3102	T5457	T5456	pobj	controls,to
R3103	T5458	T5459	punct	(,4B
R3104	T5459	T5424	parataxis	4B,reduced
R3105	T5460	T5459	compound	Figure,4B
R3106	T5461	T5459	punct	),4B
R3107	T5462	T5424	punct	.,reduced
R3108	T5464	T5465	advcl	Taken,were
R3109	T5466	T5464	advmod	together,Taken
R311	T1110	T1108	conj	mesenchyme,epithelium
R3110	T5467	T5465	punct	", ",were
R3111	T5468	T5469	det	these,data
R3112	T5469	T5465	nsubj	data,were
R3113	T5470	T5465	acomp	consistent,were
R3114	T5471	T5470	prep	with,consistent
R3115	T5472	T5473	poss	our,results
R3116	T5473	T5471	pobj	results,with
R3117	T5474	T5473	acl	obtained,results
R3118	T5475	T5474	prep	from,obtained
R3119	T5476	T5477	compound	immunofluorescence,microscopy
R312	T1111	T1112	aux	to,specify
R3120	T5477	T5475	pobj	microscopy,from
R3121	T5478	T5465	punct	.,were
R3122	T5480	T5481	advmod	A,priori
R3123	T5481	T5482	advmod	priori,be
R3124	T5483	T5482	punct	", ",be
R3125	T5484	T5485	det	the,decrease
R3126	T5485	T5482	nsubj	decrease,be
R3127	T5486	T5485	prep	in,decrease
R3128	T5487	T5488	compound	α,catenin
R3129	T5488	T5490	compound	catenin,levels
R313	T1112	T1103	advcl	specify,exchange
R3130	T5489	T5488	punct	-,catenin
R3131	T5490	T5486	pobj	levels,in
R3132	T5491	T5482	aux	could,be
R3133	T5492	T5482	prep	due,be
R3134	T5493	T5492	pcomp	to,due
R3135	T5494	T5495	preconj	either,repression
R3136	T5495	T5492	pobj	repression,due
R3137	T5496	T5495	amod	direct,repression
R3138	T5497	T5495	amod	transcriptional,repression
R3139	T5498	T5495	prep	by,repression
R314	T1113	T1114	det	the,identity
R3140	T5499	T5498	pobj	Snail,by
R3141	T5500	T5495	cc	or,repression
R3142	T5501	T5495	conj	perturbations,repression
R3143	T5502	T5501	prep	in,perturbations
R3144	T5503	T5504	compound	AJ,formation
R3145	T5504	T5502	pobj	formation,in
R3146	T5505	T5501	acl	caused,perturbations
R3147	T5506	T5505	agent	by,caused
R3148	T5507	T5508	det	the,decrease
R3149	T5508	T5506	pobj	decrease,by
R315	T1114	T1112	dobj	identity,specify
R3150	T5509	T5508	prep	in,decrease
R3151	T5510	T5511	compound	E,cadherin
R3152	T5511	T5513	compound	cadherin,expression
R3153	T5512	T5511	punct	-,cadherin
R3154	T5513	T5509	pobj	expression,in
R3155	T5514	T5513	compound	gene,expression
R3156	T5515	T5482	punct	.,be
R3157	T5517	T5518	aux	To,distinguish
R3158	T5518	T5519	advcl	distinguish,tested
R3159	T5520	T5518	prep	between,distinguish
R316	T1115	T1114	prep	of,identity
R3160	T5521	T5522	det	these,possibilities
R3161	T5522	T5520	pobj	possibilities,between
R3162	T5523	T5519	punct	", ",tested
R3163	T5524	T5519	nsubj	we,tested
R3164	T5525	T5526	mark	whether,restored
R3165	T5526	T5519	ccomp	restored,tested
R3166	T5527	T5528	compound	α,catenin
R3167	T5528	T5530	compound	catenin,levels
R3168	T5529	T5528	punct	-,catenin
R3169	T5530	T5526	nsubjpass	levels,restored
R317	T1116	T1117	det	the,organ
R3170	T5531	T5526	aux	could,restored
R3171	T5532	T5526	auxpass	be,restored
R3172	T5533	T5526	agent	by,restored
R3173	T5534	T5535	amod	exogenous,expression
R3174	T5535	T5533	pobj	expression,by
R3175	T5536	T5535	prep	of,expression
R3176	T5537	T5538	compound	E,cadherin
R3177	T5538	T5536	pobj	cadherin,of
R3178	T5539	T5538	punct	-,cadherin
R3179	T5540	T5526	prep	in,restored
R318	T1117	T1115	pobj	organ,of
R3180	T5541	T5542	npadvmod	Snail,expressing
R3181	T5542	T5544	amod	expressing,keratinocytes
R3182	T5543	T5542	punct	-,expressing
R3183	T5544	T5540	pobj	keratinocytes,in
R3184	T5545	T5519	punct	.,tested
R3185	T5547	T5548	mark	As,shown
R3186	T5548	T5549	advcl	shown,displayed
R3187	T5550	T5548	prep	in,shown
R3188	T5551	T5552	compound	Figure,4C
R3189	T5552	T5550	pobj	4C,in
R319	T1118	T1119	dep	that,form
R3190	T5553	T5549	punct	", ",displayed
R3191	T5554	T5555	advmod	transiently,transfected
R3192	T5555	T5556	amod	transfected,keratinocytes
R3193	T5556	T5549	nsubj	keratinocytes,displayed
R3194	T5557	T5556	acl	expressing,keratinocytes
R3195	T5558	T5559	npadvmod	HA,tagged
R3196	T5559	T5561	amod	tagged,Snail
R3197	T5560	T5559	punct	-,tagged
R3198	T5561	T5557	dobj	Snail,expressing
R3199	T5562	T5563	det	a,loss
R320	T1119	T1117	relcl	form,organ
R3200	T5563	T5549	dobj	loss,displayed
R3201	T5564	T5563	prep	of,loss
R3202	T5565	T5566	compound	E,cadherin
R3203	T5566	T5564	pobj	cadherin,of
R3204	T5567	T5566	punct	-,cadherin
R3205	T5568	T5566	cc	and,cadherin
R3206	T5569	T5570	compound	α,catenin
R3207	T5570	T5566	conj	catenin,cadherin
R3208	T5571	T5570	punct	-,catenin
R3209	T5572	T5549	prep	at,displayed
R321	T1120	T1119	aux	will,form
R3210	T5573	T5574	compound	cell,cell
R3211	T5574	T5576	compound	cell,borders
R3212	T5575	T5574	punct	-,cell
R3213	T5576	T5572	pobj	borders,at
R3214	T5577	T5549	punct	.,displayed
R3215	T5579	T5580	nsubj	Coexpression,enabled
R3216	T5581	T5579	prep	of,Coexpression
R3217	T5582	T5583	amod	exogenous,cadherin
R3218	T5583	T5581	pobj	cadherin,of
R3219	T5584	T5585	npadvmod	HA,tagged
R322	T1121	T1112	cc	and,specify
R3220	T5585	T5583	amod	tagged,cadherin
R3221	T5586	T5585	punct	-,tagged
R3222	T5587	T5583	compound	E,cadherin
R3223	T5588	T5583	punct	-,cadherin
R3224	T5589	T5580	preconj	not,enabled
R3225	T5590	T5589	advmod	only,not
R3226	T5591	T5592	compound	cell,cell
R3227	T5592	T5594	compound	cell,localization
R3228	T5593	T5592	punct	-,cell
R3229	T5594	T5580	dobj	localization,enabled
R323	T1122	T1123	aux	to,govern
R3230	T5595	T5594	compound	border,localization
R3231	T5596	T5594	prep	of,localization
R3232	T5597	T5598	compound	E,cadherin
R3233	T5598	T5600	compound	cadherin,protein
R3234	T5599	T5598	punct	-,cadherin
R3235	T5600	T5596	pobj	protein,of
R3236	T5601	T5580	punct	", ",enabled
R3237	T5602	T5580	cc	but,enabled
R3238	T5603	T5602	advmod	also,but
R3239	T5604	T5580	conj	rescued,enabled
R324	T1123	T1112	conj	govern,specify
R3240	T5605	T5606	det	the,staining
R3241	T5606	T5604	dobj	staining,rescued
R3242	T5607	T5608	compound	cell,cell
R3243	T5608	T5606	compound	cell,staining
R3244	T5609	T5608	punct	-,cell
R3245	T5610	T5606	compound	border,staining
R3246	T5611	T5606	prep	of,staining
R3247	T5612	T5613	compound	α,catenin
R3248	T5613	T5611	pobj	catenin,of
R3249	T5614	T5613	punct	-,catenin
R325	T1124	T1125	poss	its,growth
R3250	T5615	T5616	punct	(,4C
R3251	T5616	T5604	parataxis	4C,rescued
R3252	T5617	T5616	compound	Figure,4C
R3253	T5618	T5616	punct	),4C
R3254	T5619	T5580	punct	.,enabled
R3255	T5621	T5622	det	The,ability
R3256	T5622	T5623	nsubj	ability,argues
R3257	T5624	T5625	aux	to,restore
R3258	T5625	T5622	acl	restore,ability
R3259	T5626	T5627	compound	α,catenin
R326	T1125	T1123	dobj	growth,govern
R3260	T5627	T5625	dobj	catenin,restore
R3261	T5628	T5627	punct	-,catenin
R3262	T5629	T5627	appos	expression,catenin
R3263	T5630	T5629	cc	and,expression
R3264	T5631	T5629	conj	localization,expression
R3265	T5632	T5625	prep	under,restore
R3266	T5633	T5634	det	these,conditions
R3267	T5634	T5632	pobj	conditions,under
R3268	T5635	T5623	prep	against,argues
R3269	T5636	T5637	det	the,notion
R327	T1126	T1099	punct	.,guided
R3270	T5637	T5635	pobj	notion,against
R3271	T5638	T5639	mark	that,represses
R3272	T5639	T5637	acl	represses,notion
R3273	T5640	T5639	nsubj	Snail,represses
R3274	T5641	T5639	advmod	transcriptionally,represses
R3275	T5642	T5643	compound	α,catenin
R3276	T5643	T5639	dobj	catenin,represses
R3277	T5644	T5643	punct	-,catenin
R3278	T5645	T5623	punct	.,argues
R3279	T5647	T5648	advmod	Rather,are
R328	T1128	T1129	prep	At,are
R3280	T5649	T5648	punct	", ",are
R3281	T5650	T5651	det	the,findings
R3282	T5651	T5648	nsubj	findings,are
R3283	T5652	T5648	acomp	consistent,are
R3284	T5653	T5652	prep	with,consistent
R3285	T5654	T5655	det	a,report
R3286	T5655	T5653	pobj	report,with
R3287	T5656	T5655	amod	previous,report
R3288	T5657	T5658	mark	that,required
R3289	T5658	T5655	acl	required,report
R329	T1130	T1131	det	the,helm
R3290	T5659	T5660	compound	E,cadherin
R3291	T5660	T5658	nsubjpass	cadherin,required
R3292	T5661	T5660	punct	-,cadherin
R3293	T5662	T5658	auxpass	is,required
R3294	T5663	T5658	prep	for,required
R3295	T5664	T5665	det	the,translation
R3296	T5665	T5663	pobj	translation,for
R3297	T5666	T5665	prep	of,translation
R3298	T5667	T5668	compound	α,catenin
R3299	T5668	T5670	compound	catenin,mRNA
R330	T1131	T1128	pobj	helm,At
R3300	T5669	T5668	punct	-,catenin
R3301	T5670	T5666	pobj	mRNA,of
R3302	T5671	T5672	punct	[,22
R3303	T5672	T5648	parataxis	22,are
R3304	T5673	T5672	punct	],22
R3305	T5674	T5648	punct	.,are
R3306	T5676	T5677	prep	Despite,displayed
R3307	T5678	T5679	det	the,reductions
R3308	T5679	T5676	pobj	reductions,Despite
R3309	T5680	T5679	prep	in,reductions
R331	T1132	T1131	prep	of,helm
R3310	T5681	T5682	compound	AJ,markers
R3311	T5682	T5680	pobj	markers,in
R3312	T5683	T5677	punct	", ",displayed
R3313	T5684	T5685	compound	Tg,skin
R3314	T5685	T5677	nsubj	skin,displayed
R3315	T5686	T5677	advmod	still,displayed
R3316	T5687	T5688	amod	sealed,membranes
R3317	T5688	T5677	dobj	membranes,displayed
R3318	T5689	T5688	cc	and,membranes
R3319	T5690	T5691	amod	intercellular,junctions
R332	T1133	T1134	det	these,pathways
R3320	T5691	T5688	conj	junctions,membranes
R3321	T5692	T5693	dep	that,were
R3322	T5693	T5691	relcl	were,junctions
R3323	T5694	T5693	advmod	largely,were
R3324	T5695	T5693	acomp	intact,were
R3325	T5696	T5693	punct	", ",were
R3326	T5697	T5698	mark	as,judged
R3327	T5698	T5693	advcl	judged,were
R3328	T5699	T5698	prep	by,judged
R3329	T5700	T5701	amod	ultrastructural,analyses
R333	T1134	T1132	pobj	pathways,of
R3330	T5701	T5699	pobj	analyses,by
R3331	T5702	T5703	punct	(,data
R3332	T5703	T5677	meta	data,displayed
R3333	T5704	T5703	amod	unpublished,data
R3334	T5705	T5703	punct	),data
R3335	T5706	T5677	punct	.,displayed
R3336	T5708	T5709	prep	In,resembled
R3337	T5710	T5711	det	this,respect
R3338	T5711	T5708	pobj	respect,In
R3339	T5712	T5709	punct	", ",resembled
R334	T1135	T1136	amod	molecular,communication
R3340	T5713	T5714	det	the,epithelium
R3341	T5714	T5709	nsubj	epithelium,resembled
R3342	T5715	T5714	compound	skin,epithelium
R3343	T5716	T5709	dobj	that,resembled
R3344	T5717	T5716	prep	of,that
R3345	T5718	T5719	det	the,bud
R3346	T5719	T5717	pobj	bud,of
R3347	T5720	T5719	compound	hair,bud
R3348	T5721	T5719	punct	", ",bud
R3349	T5722	T5723	advmod	where,is
R335	T1136	T1134	compound	communication,pathways
R3350	T5723	T5719	relcl	is,bud
R3351	T5724	T5725	det	the,regulation
R3352	T5725	T5723	nsubj	regulation,is
R3353	T5726	T5725	amod	down,regulation
R3354	T5727	T5725	punct	-,regulation
R3355	T5728	T5725	prep	in,regulation
R3356	T5729	T5730	compound	junction,proteins
R3357	T5730	T5728	pobj	proteins,in
R3358	T5731	T5723	acomp	permissive,is
R3359	T5732	T5723	prep	for,is
R336	T1137	T1129	nsubj	Wnts,are
R3360	T5733	T5734	compound	cell,cell
R3361	T5734	T5736	compound	cell,remodeling
R3362	T5735	T5734	punct	-,cell
R3363	T5736	T5732	pobj	remodeling,for
R3364	T5737	T5723	prep	without,is
R3365	T5738	T5737	pcomp	abrogating,without
R3366	T5739	T5740	amod	intercellular,adhesion
R3367	T5740	T5738	dobj	adhesion,abrogating
R3368	T5741	T5709	punct	.,resembled
R3369	T5743	T5744	det	The,similarities
R337	T1138	T1137	punct	", ",Wnts
R3370	T5744	T5745	nsubj	similarities,extended
R3371	T5746	T5744	prep	between,similarities
R3372	T5747	T5748	compound	Snail,epidermis
R3373	T5748	T5746	pobj	epidermis,between
R3374	T5749	T5748	compound	Tg,epidermis
R3375	T5750	T5748	cc	and,epidermis
R3376	T5751	T5752	compound	hair,buds
R3377	T5752	T5748	conj	buds,epidermis
R3378	T5753	T5745	prep	to,extended
R3379	T5754	T5755	det	the,state
R338	T1139	T1140	nmod	bone,proteins
R3380	T5755	T5753	pobj	state,to
R3381	T5756	T5755	amod	hyperproliferative,state
R3382	T5757	T5745	punct	", ",extended
R3383	T5758	T5745	advcl	leading,extended
R3384	T5759	T5758	dobj	us,leading
R3385	T5760	T5761	aux	to,wonder
R3386	T5761	T5758	xcomp	wonder,leading
R3387	T5762	T5763	mark	whether,contribute
R3388	T5763	T5761	ccomp	contribute,wonder
R3389	T5764	T5765	det	the,regulation
R339	T1140	T1137	appos	proteins,Wnts
R3390	T5765	T5763	nsubj	regulation,contribute
R3391	T5766	T5765	amod	down,regulation
R3392	T5767	T5765	punct	-,regulation
R3393	T5768	T5765	prep	of,regulation
R3394	T5769	T5770	compound	AJ,proteins
R3395	T5770	T5768	pobj	proteins,of
R3396	T5771	T5763	aux	might,contribute
R3397	T5772	T5763	prep	to,contribute
R3398	T5773	T5774	det	this,condition
R3399	T5774	T5772	pobj	condition,to
R340	T1141	T1140	amod	morphogenic,proteins
R3400	T5775	T5745	punct	.,extended
R3401	T5777	T5778	prep	Given,used
R3402	T5779	T5780	det	the,increase
R3403	T5780	T5777	pobj	increase,Given
R3404	T5781	T5780	prep	in,increase
R3405	T5782	T5783	compound	pMAPK,staining
R3406	T5783	T5781	pobj	staining,in
R3407	T5784	T5780	prep	in,increase
R3408	T5785	T5786	compound	Snail,epidermis
R3409	T5786	T5784	pobj	epidermis,in
R341	T1142	T1140	punct	(,proteins
R3410	T5787	T5786	compound	Tg,epidermis
R3411	T5788	T5789	punct	(,see
R3412	T5789	T5780	parataxis	see,increase
R3413	T5790	T5791	compound	Figure,2G
R3414	T5791	T5789	dobj	2G,see
R3415	T5792	T5789	punct	),see
R3416	T5793	T5778	punct	", ",used
R3417	T5794	T5778	nsubj	we,used
R3418	T5795	T5796	compound	pMAPK,levels
R3419	T5796	T5778	dobj	levels,used
R342	T1143	T1140	appos	BMPs,proteins
R3420	T5797	T5778	prep	as,used
R3421	T5798	T5799	poss	our,assay
R3422	T5799	T5797	pobj	assay,as
R3423	T5800	T5801	aux	to,test
R3424	T5801	T5778	advcl	test,used
R3425	T5802	T5803	mark	whether,contributed
R3426	T5803	T5801	ccomp	contributed,test
R3427	T5804	T5805	det	the,loss
R3428	T5805	T5803	nsubj	loss,contributed
R3429	T5806	T5805	prep	of,loss
R343	T1144	T1137	punct	),Wnts
R3430	T5807	T5808	compound	E,cadherin
R3431	T5808	T5806	pobj	cadherin,of
R3432	T5809	T5808	punct	-,cadherin
R3433	T5810	T5803	prep	to,contributed
R3434	T5811	T5812	det	the,increase
R3435	T5812	T5810	pobj	increase,to
R3436	T5813	T5814	npadvmod	Snail,mediated
R3437	T5814	T5812	amod	mediated,increase
R3438	T5815	T5814	punct	-,mediated
R3439	T5816	T5812	prep	in,increase
R344	T1145	T1137	punct	", ",Wnts
R3440	T5817	T5816	pobj	proliferation,in
R3441	T5818	T5778	punct	.,used
R3442	T5820	T5821	advcl	Consistent,exhibited
R3443	T5822	T5820	prep	with,Consistent
R3444	T5823	T5824	poss	our,observations
R3445	T5824	T5822	pobj	observations,with
R3446	T5825	T5826	advmod	in,vivo
R3447	T5826	T5824	amod	vivo,observations
R3448	T5827	T5821	punct	", ",exhibited
R3449	T5828	T5829	amod	transfected,keratinocytes
R345	T1146	T1147	amod	transforming,βs
R3450	T5829	T5821	nsubj	keratinocytes,exhibited
R3451	T5830	T5829	acl	expressing,keratinocytes
R3452	T5831	T5830	dobj	Snail,expressing
R3453	T5832	T5833	det	a,increase
R3454	T5833	T5821	dobj	increase,exhibited
R3455	T5834	T5833	amod	substantial,increase
R3456	T5835	T5833	prep	in,increase
R3457	T5836	T5837	compound	pMAPK,levels
R3458	T5837	T5835	pobj	levels,in
R3459	T5838	T5833	amod	relative,increase
R346	T1147	T1137	appos	βs,Wnts
R3460	T5839	T5838	prep	to,relative
R3461	T5840	T5841	compound	control,cells
R3462	T5841	T5839	pobj	cells,to
R3463	T5842	T5843	punct	(,4D
R3464	T5843	T5821	parataxis	4D,exhibited
R3465	T5844	T5843	compound	Figure,4D
R3466	T5845	T5843	punct	),4D
R3467	T5846	T5821	punct	.,exhibited
R3468	T5848	T5849	nsubj	Coexpression,appeared
R3469	T5850	T5848	prep	of,Coexpression
R347	T1148	T1147	compound	growth,βs
R3470	T5851	T5852	compound	E,cadherin
R3471	T5852	T5850	pobj	cadherin,of
R3472	T5853	T5852	punct	-,cadherin
R3473	T5854	T5848	prep	with,Coexpression
R3474	T5855	T5854	pobj	Snail,with
R3475	T5856	T5857	aux	to,abrogate
R3476	T5857	T5849	xcomp	abrogate,appeared
R3477	T5858	T5859	det	this,effect
R3478	T5859	T5857	dobj	effect,abrogate
R3479	T5860	T5849	punct	.,appeared
R348	T1149	T1147	compound	factor,βs
R3480	T5862	T5863	advmod	Together,raised
R3481	T5864	T5863	punct	", ",raised
R3482	T5865	T5866	det	these,findings
R3483	T5866	T5863	nsubj	findings,raised
R3484	T5867	T5868	det	the,possibility
R3485	T5868	T5863	dobj	possibility,raised
R3486	T5869	T5870	mark	that,participate
R3487	T5870	T5868	acl	participate,possibility
R3488	T5871	T5872	det	an,protein
R3489	T5872	T5870	nsubj	protein,participate
R349	T1150	T1147	punct	(,βs
R3490	T5873	T5874	npadvmod	AJ,associated
R3491	T5874	T5872	amod	associated,protein
R3492	T5875	T5874	punct	-,associated
R3493	T5876	T5877	dep	that,sequestered
R3494	T5877	T5872	relcl	sequestered,protein
R3495	T5878	T5877	auxpass	is,sequestered
R3496	T5879	T5877	advmod	normally,sequestered
R3497	T5880	T5877	prep	at,sequestered
R3498	T5881	T5882	det	the,membrane
R3499	T5882	T5880	pobj	membrane,at
R350	T1151	T1152	compound	TGF,βs
R3500	T5883	T5882	compound	plasma,membrane
R3501	T5884	T5870	aux	may,participate
R3502	T5885	T5870	prep	in,participate
R3503	T5886	T5887	det	a,pathway
R3504	T5887	T5885	pobj	pathway,in
R3505	T5888	T5887	compound	proliferation,pathway
R3506	T5889	T5887	compound	signaling,pathway
R3507	T5890	T5891	advmod	when,deconstructed
R3508	T5891	T5870	advcl	deconstructed,participate
R3509	T5892	T5891	nsubjpass	AJs,deconstructed
R351	T1152	T1147	appos	βs,βs
R3510	T5893	T5891	auxpass	are,deconstructed
R3511	T5894	T5863	punct	.,raised
R3512	T5896	T5897	amod	Numerous,studies
R3513	T5897	T5898	nsubj	studies,correlated
R3514	T5899	T5898	aux	have,correlated
R3515	T5900	T5901	det	a,regulation
R3516	T5901	T5898	dobj	regulation,correlated
R3517	T5902	T5901	amod	down,regulation
R3518	T5903	T5901	punct	-,regulation
R3519	T5904	T5901	prep	of,regulation
R352	T1153	T1152	punct	-,βs
R3520	T5905	T5906	compound	E,cadherin
R3521	T5906	T5904	pobj	cadherin,of
R3522	T5907	T5906	punct	-,cadherin
R3523	T5908	T5898	prep	with,correlated
R3524	T5909	T5910	det	a,translocation
R3525	T5910	T5908	pobj	translocation,with
R3526	T5911	T5910	prep	of,translocation
R3527	T5912	T5913	compound	β,catenin
R3528	T5913	T5911	pobj	catenin,of
R3529	T5914	T5913	punct	-,catenin
R353	T1154	T1137	punct	),Wnts
R3530	T5915	T5910	prep	to,translocation
R3531	T5916	T5917	det	the,nucleus
R3532	T5917	T5915	pobj	nucleus,to
R3533	T5918	T5910	cc	and,translocation
R3534	T5919	T5920	det	a,transactivation
R3535	T5920	T5910	conj	transactivation,translocation
R3536	T5921	T5920	prep	of,transactivation
R3537	T5922	T5921	pobj	genes,of
R3538	T5923	T5924	dep	that,regulated
R3539	T5924	T5922	relcl	regulated,genes
R354	T1155	T1137	punct	", ",Wnts
R3540	T5925	T5924	auxpass	are,regulated
R3541	T5926	T5924	agent	by,regulated
R3542	T5927	T5928	det	the,family
R3543	T5928	T5926	pobj	family,by
R3544	T5929	T5928	nmod	LEF,family
R3545	T5930	T5929	punct	-,LEF
R3546	T5931	T5929	nummod	1,LEF
R3547	T5932	T5929	punct	/,LEF
R3548	T5933	T5934	compound	T,cell
R3549	T5934	T5935	compound	cell,factor
R355	T1156	T1137	cc	and,Wnts
R3550	T5935	T5929	appos	factor,LEF
R3551	T5936	T5935	punct	(,factor
R3552	T5937	T5935	appos	TCF,factor
R3553	T5938	T5928	punct	),family
R3554	T5939	T5928	prep	of,family
R3555	T5940	T5941	npadvmod	DNA,binding
R3556	T5941	T5942	amod	binding,proteins
R3557	T5942	T5939	pobj	proteins,of
R3558	T5943	T5944	punct	[,25
R3559	T5944	T5898	parataxis	25,correlated
R356	T1157	T1158	compound	fibroblast,factors
R3560	T5945	T5944	nummod	23,25
R3561	T5946	T5944	punct	",",25
R3562	T5947	T5944	nummod	24,25
R3563	T5948	T5944	punct	",",25
R3564	T5949	T5944	punct	],25
R3565	T5950	T5898	punct	.,correlated
R3566	T5952	T5953	det	The,presence
R3567	T5953	T5954	nsubj	presence,was
R3568	T5955	T5953	prep	of,presence
R3569	T5956	T5957	amod	nuclear,D
R357	T1158	T1137	conj	factors,Wnts
R3570	T5957	T5955	pobj	D,of
R3571	T5958	T5957	compound	cyclin,D
R3572	T5959	T5953	prep	in,presence
R3573	T5960	T5961	amod	hyperproliferative,epidermis
R3574	T5961	T5959	pobj	epidermis,in
R3575	T5962	T5961	compound	Snail,epidermis
R3576	T5963	T5961	compound	Tg,epidermis
R3577	T5964	T5965	advmod	particularly,intriguing
R3578	T5965	T5954	acomp	intriguing,was
R3579	T5966	T5967	mark	since,reported
R358	T1159	T1158	compound	growth,factors
R3580	T5967	T5954	advcl	reported,was
R3581	T5968	T5969	amod	prior,studies
R3582	T5969	T5967	nsubj	studies,reported
R3583	T5970	T5967	aux	have,reported
R3584	T5971	T5972	compound	cyclin,D
R3585	T5972	T5973	compound	D,gene
R3586	T5973	T5967	dobj	gene,reported
R3587	T5974	T5967	prep	as,reported
R3588	T5975	T5976	det	a,target
R3589	T5976	T5974	pobj	target,as
R359	T1160	T1158	punct	(,factors
R3590	T5977	T5976	amod	direct,target
R3591	T5978	T5976	prep	of,target
R3592	T5979	T5980	nmod	TCF,β
R3593	T5980	T5982	nmod	β,transcription
R3594	T5981	T5980	punct	/,β
R3595	T5982	T5978	pobj	transcription,of
R3596	T5983	T5980	punct	-,β
R3597	T5984	T5980	appos	catenin,β
R3598	T5985	T5986	punct	[,26
R3599	T5986	T5954	parataxis	26,was
R360	T1161	T1158	appos	FGFs,factors
R3600	T5987	T5986	punct	],26
R3601	T5988	T5954	punct	.,was
R3602	T5990	T5991	nsubj	This,said
R3603	T5991	T5992	advcl	said,detect
R3604	T5993	T5992	punct	", ",detect
R3605	T5994	T5992	nsubj	we,detect
R3606	T5995	T5992	aux	did,detect
R3607	T5996	T5992	neg	not,detect
R3608	T5997	T5998	amod	nuclear,catenin
R3609	T5998	T5992	dobj	catenin,detect
R361	T1162	T1129	punct	),are
R3610	T5999	T5998	compound	β,catenin
R3611	T6000	T5998	punct	-,catenin
R3612	T6001	T5992	prep	in,detect
R3613	T6002	T6003	poss	our,epidermis
R3614	T6003	T6001	pobj	epidermis,in
R3615	T6004	T6003	compound	Tg,epidermis
R3616	T6005	T5992	punct	", ",detect
R3617	T6006	T5992	cc	and,detect
R3618	T6007	T6008	csubj	mating,gave
R3619	T6008	T5992	conj	gave,detect
R362	T1163	T1129	punct	.,are
R3620	T6009	T6010	det	the,mice
R3621	T6010	T6007	dobj	mice,mating
R3622	T6011	T6010	compound	Snail,mice
R3623	T6012	T6010	compound	Tg,mice
R3624	T6013	T6007	prep	against,mating
R3625	T6014	T6015	det	the,mouse
R3626	T6015	T6013	pobj	mouse,against
R3627	T6016	T6015	compound	TOPGal,mouse
R3628	T6017	T6015	compound	reporter,mouse
R3629	T6018	T6019	punct	[,20
R363	T1165	T1166	prep	Through,trigger
R3630	T6019	T6007	parataxis	20,mating
R3631	T6020	T6019	punct	],20
R3632	T6021	T6022	det	no,signs
R3633	T6022	T6008	dobj	signs,gave
R3634	T6023	T6022	prep	of,signs
R3635	T6024	T6025	amod	ectopic,activity
R3636	T6025	T6023	pobj	activity,of
R3637	T6026	T6025	nmod	LEF,activity
R3638	T6027	T6026	punct	-,LEF
R3639	T6028	T6026	nummod	1,LEF
R364	T1167	T1165	pobj	activation,Through
R3640	T6029	T6026	punct	/,LEF
R3641	T6030	T6026	appos	Tcf,LEF
R3642	T6031	T6026	punct	/,LEF
R3643	T6032	T6033	compound	β,catenin
R3644	T6033	T6026	appos	catenin,LEF
R3645	T6034	T6033	punct	-,catenin
R3646	T6035	T6036	punct	(,data
R3647	T6036	T6008	meta	data,gave
R3648	T6037	T6036	amod	unpublished,data
R3649	T6038	T6036	punct	),data
R365	T1168	T1167	prep	of,activation
R3650	T6039	T5992	punct	.,detect
R3651	T6041	T6042	nsubj	We,turned
R3652	T6043	T6042	advmod	next,turned
R3653	T6044	T6042	prep	to,turned
R3654	T6045	T6046	det	the,presence
R3655	T6093	T6091	nummod	2,protein
R3656	T6046	T6044	pobj	presence,to
R3657	T6094	T6091	punct	(,protein
R3658	T6095	T6091	appos	Grb,protein
R3659	T6047	T6046	prep	of,presence
R366	T1169	T1170	compound	cell,surface
R3660	T6096	T6095	punct	-,Grb
R3661	T6097	T6095	nummod	2,Grb
R3662	T6048	T6049	amod	cytoplasmic,Ajuba
R3663	T6098	T6091	punct	),protein
R3664	T6099	T6091	punct	/,protein
R3665	T6100	T6091	appos	son,protein
R3666	T6049	T6047	pobj	Ajuba,of
R3667	T6101	T6100	prep	of,son
R3668	T6102	T6101	pobj	sevenless,of
R3669	T6103	T6100	punct	(,son
R367	T1170	T1171	compound	surface,receptors
R3670	T6050	T6042	prep	for,turned
R3671	T6104	T6100	appos	Sos,son
R3672	T6105	T6091	punct	),protein
R3673	T6106	T6091	punct	", ",protein
R3674	T6051	T6052	det	a,link
R3675	T6107	T6108	det	the,factor
R3676	T6108	T6091	appos	factor,protein
R3677	T6109	T6108	compound	nucleotide,factor
R3678	T6052	T6050	pobj	link,for
R3679	T6110	T6108	compound	exchange,factor
R368	T1171	T1168	pobj	receptors,of
R3680	T6111	T6108	prep	for,factor
R3681	T6112	T6111	pobj	Ras,for
R3682	T6053	T6052	amod	possible,link
R3683	T6113	T6112	punct	", ",Ras
R3684	T6114	T6115	dep	which,is
R3685	T6115	T6112	relcl	is,Ras
R3686	T6054	T6052	amod	mechanistic,link
R3687	T6116	T6115	advmod	upstream,is
R3688	T6117	T6116	prep	from,upstream
R3689	T6118	T6117	pobj	activation,from
R369	T1172	T1171	compound	transmembrane,receptors
R3690	T6119	T6118	prep	of,activation
R3691	T6055	T6052	prep	to,link
R3692	T6120	T6119	pobj	MAPK,of
R3693	T6121	T6122	punct	[,9
R3694	T6056	T6057	det	the,increase
R3695	T6122	T6067	parataxis	9,associate
R3696	T6123	T6122	punct	],9
R3697	T6124	T6067	punct	.,associate
R3698	T6057	T6055	pobj	increase,to
R3699	T6126	T6127	prep	Given,examined
R370	T1173	T1166	punct	", ",trigger
R3700	T6058	T6057	amod	proliferative,increase
R3701	T6128	T6129	det	the,increase
R3702	T6129	T6126	pobj	increase,Given
R3703	T6130	T6129	prep	in,increase
R3704	T6131	T6132	compound	pMAPK,staining
R3705	T6059	T6057	prep	in,increase
R3706	T6132	T6130	pobj	staining,in
R3707	T6060	T6061	poss	our,epidermis
R3708	T6133	T6129	prep	in,increase
R3709	T6134	T6135	compound	Tg,skin
R371	T1174	T1175	det	these,molecules
R3710	T6061	T6059	pobj	epidermis,in
R3711	T6135	T6133	pobj	skin,in
R3712	T6136	T6127	punct	", ",examined
R3713	T6137	T6127	nsubj	we,examined
R3714	T6062	T6061	compound	Snail,epidermis
R3715	T6138	T6139	det	the,possibility
R3716	T6139	T6127	dobj	possibility,examined
R3717	T6140	T6141	mark	that,changed
R3718	T6063	T6061	compound	Tg,epidermis
R3719	T6141	T6139	acl	changed,possibility
R372	T1175	T1166	nsubj	molecules,trigger
R3720	T6142	T6141	nsubj	Ajuba,changed
R3721	T6143	T6141	aux	might,changed
R3722	T6064	T6042	punct	.,turned
R3723	T6144	T6141	aux	have,changed
R3724	T6145	T6146	poss	its,partner
R3725	T6146	T6141	dobj	partner,changed
R3726	T6066	T6067	prep	In,associate
R3727	T6147	T6146	compound	binding,partner
R3728	T6148	T6141	prep	in,changed
R3729	T6149	T6150	npadvmod	Snail,expressing
R373	T1176	T1175	amod	external,molecules
R3730	T6150	T6152	amod	expressing,epidermis
R3731	T6151	T6150	punct	-,expressing
R3732	T6068	T6066	pobj	addition,In
R3733	T6152	T6148	pobj	epidermis,in
R3734	T6153	T6127	punct	.,examined
R3735	T6155	T6156	advmod	Interestingly,detected
R3736	T6069	T6068	prep	to,addition
R3737	T6157	T6156	punct	", ",detected
R3738	T6070	T6071	poss	its,ability
R3739	T6158	T6156	nsubjpass	Ajuba,detected
R374	T1177	T1175	compound	signaling,molecules
R3740	T6159	T6156	auxpass	was,detected
R3741	T6160	T6156	advmod	readily,detected
R3742	T6071	T6069	pobj	ability,to
R3743	T6161	T6156	prep	in,detected
R3744	T6162	T6163	amod	anti-Grb,immunoprecipitates
R3745	T6163	T6161	pobj	immunoprecipitates,in
R3746	T6072	T6071	amod	documented,ability
R3747	T6164	T6162	punct	-,anti-Grb
R3748	T6165	T6162	advmod	2,anti-Grb
R3749	T6073	T6074	aux	to,bind
R375	T1178	T1179	amod	distinct,cascades
R3750	T6166	T6163	prep	of,immunoprecipitates
R3751	T6167	T6168	compound	protein,lysates
R3752	T6168	T6166	pobj	lysates,of
R3753	T6074	T6071	acl	bind,ability
R3754	T6169	T6168	prep	from,lysates
R3755	T6170	T6169	pobj	skins,from
R3756	T6171	T6170	prep	of,skins
R3757	T6075	T6076	compound	α,catenin
R3758	T6172	T6173	compound	Snail,mice
R3759	T6173	T6171	pobj	mice,of
R376	T1179	T1166	dobj	cascades,trigger
R3760	T6076	T6074	dobj	catenin,bind
R3761	T6174	T6173	compound	Tg,mice
R3762	T6175	T6169	cc	but,from
R3763	T6176	T6175	neg	not,but
R3764	T6077	T6076	punct	-,catenin
R3765	T6177	T6169	conj	from,from
R3766	T6178	T6179	det	the,animals
R3767	T6078	T6079	punct	[,10
R3768	T6179	T6177	pobj	animals,from
R3769	T6180	T6179	amod	corresponding,animals
R377	T1180	T1179	prep	of,cascades
R3770	T6181	T6182	amod	wild,type
R3771	T6079	T6068	parataxis	10,addition
R3772	T6182	T6179	nmod	type,animals
R3773	T6183	T6182	punct	-,type
R3774	T6184	T6182	punct	(,type
R3775	T6080	T6079	punct	],10
R3776	T6185	T6182	appos	WT,type
R3777	T6186	T6179	punct	),animals
R3778	T6081	T6067	punct	", ",associate
R3779	T6187	T6188	punct	(,4E
R378	T1181	T1182	amod	intracellular,events
R3780	T6188	T6156	parataxis	4E,detected
R3781	T6189	T6188	compound	Figure,4E
R3782	T6082	T6067	nsubj	Ajuba,associate
R3783	T6190	T6188	punct	),4E
R3784	T6191	T6156	punct	.,detected
R3785	T6083	T6067	aux	can,associate
R3786	T6193	T6194	advmod	When,repeated
R3787	T6194	T6198	advcl	repeated,detected
R3788	T6195	T6196	det	these,experiments
R3789	T6084	T6067	advmod	also,associate
R379	T1182	T1180	pobj	events,of
R3790	T6196	T6194	nsubjpass	experiments,repeated
R3791	T6197	T6194	auxpass	were,repeated
R3792	T6085	T6067	prep	with,associate
R3793	T6086	T6087	compound	growth,factor
R3794	T6087	T6088	compound	factor,receptor
R3795	T6199	T6194	prep	with,repeated
R3796	T6088	T6089	npadvmod	receptor,bound
R3797	T6200	T6201	compound	α,catenin
R3798	T6201	T6203	npadvmod	catenin,null
R3799	T6202	T6201	punct	-,catenin
R380	T1183	T1184	dep	that,culminate
R3800	T6089	T6091	amod	bound,protein
R3801	T6203	T6205	amod	null,epidermis
R3802	T6204	T6203	punct	-,null
R3803	T6205	T6199	pobj	epidermis,with
R3804	T6090	T6089	punct	-,bound
R3805	T6206	T6198	punct	", ",detected
R3806	T6207	T6208	det	a,association
R3807	T6208	T6198	nsubjpass	association,detected
R3808	T6209	T6208	amod	similar,association
R3809	T6210	T6211	nmod	Grb,Ajuba
R381	T1184	T1179	relcl	culminate,cascades
R3810	T6211	T6208	compound	Ajuba,association
R3811	T6091	T6085	pobj	protein,with
R3812	T6212	T6211	punct	-,Ajuba
R3813	T6213	T6211	nummod	2,Ajuba
R3814	T6214	T6211	punct	-,Ajuba
R3815	T6215	T6198	auxpass	was,detected
R3816	T6092	T6091	punct	-,protein
R3817	T6216	T6198	punct	", ",detected
R3818	T6217	T6198	cc	and,detected
R3819	T6218	T6219	advmod	again,detected
R382	T1185	T1184	prep	in,culminate
R3820	T6303	T6286	acomp	relevant,is
R3821	T6219	T6198	conj	detected,detected
R3822	T6220	T6219	punct	", ",detected
R3823	T6221	T6222	det	this,interaction
R3824	T6222	T6219	nsubjpass	interaction,detected
R3825	T6223	T6219	auxpass	was,detected
R3826	T6304	T6303	prep	to,relevant
R3827	T6224	T6219	neg	not,detected
R3828	T6225	T6219	prep	in,detected
R3829	T6226	T6227	det	the,extracts
R383	T1186	T1185	pobj	changes,in
R3830	T6227	T6225	pobj	extracts,in
R3831	T6305	T6306	det	the,state
R3832	T6228	T6227	compound	protein,extracts
R3833	T6229	T6227	prep	from,extracts
R3834	T6230	T6231	compound	control,skin
R3835	T6306	T6304	pobj	state,to
R3836	T6231	T6229	pobj	skin,from
R3837	T6232	T6231	compound	littermate,skin
R3838	T6233	T6234	punct	(,4E
R3839	T6307	T6306	amod	hyperproliferative,state
R384	T1187	T1186	prep	in,changes
R3840	T6234	T6219	parataxis	4E,detected
R3841	T6235	T6234	compound	Figure,4E
R3842	T6236	T6234	punct	),4E
R3843	T6308	T6306	prep	of,state
R3844	T6237	T6198	punct	.,detected
R3845	T6239	T6240	advmod	Together,demonstrate
R3846	T6309	T6310	det	a,keratinocyte
R3847	T6241	T6240	punct	", ",demonstrate
R3848	T6242	T6243	det	these,data
R3849	T6243	T6240	nsubj	data,demonstrate
R385	T1188	T1189	compound	gene,expression
R3850	T6310	T6308	pobj	keratinocyte,of
R3851	T6244	T6245	mark	that,allows
R3852	T6311	T6301	punct	", ",expected
R3853	T6245	T6240	ccomp	allows,demonstrate
R3854	T6246	T6247	det	the,reduction
R3855	T6312	T6301	advmod	then,expected
R3856	T6247	T6245	nsubj	reduction,allows
R3857	T6248	T6247	prep	in,reduction
R3858	T6249	T6250	compound	α,catenin
R3859	T6250	T6252	compound	catenin,levels
R386	T1189	T1187	pobj	expression,in
R3860	T6251	T6250	punct	-,catenin
R3861	T6252	T6248	pobj	levels,in
R3862	T6253	T6247	punct	", ",reduction
R3863	T6313	T6301	nsubjpass	overexpression,expected
R3864	T6254	T6255	preconj	either,by
R3865	T6255	T6247	prep	by,reduction
R3866	T6256	T6257	npadvmod	Snail,mediated
R3867	T6314	T6313	prep	of,overexpression
R3868	T6257	T6259	amod	mediated,regulation
R3869	T6258	T6257	punct	-,mediated
R387	T1190	T1189	punct	", ",expression
R3870	T6259	T6255	pobj	regulation,by
R3871	T6315	T6314	pobj	Ajuba,of
R3872	T6260	T6259	amod	down,regulation
R3873	T6261	T6259	punct	-,regulation
R3874	T6262	T6259	prep	of,regulation
R3875	T6316	T6301	aux	would,expected
R3876	T6263	T6264	compound	E,cadherin
R3877	T6264	T6262	pobj	cadherin,of
R3878	T6265	T6264	punct	-,cadherin
R3879	T6317	T6301	auxpass	be,expected
R388	T1191	T1189	conj	growth,expression
R3880	T6266	T6255	cc	or,by
R3881	T6267	T6255	conj	by,by
R3882	T6268	T6269	compound	α,catenin
R3883	T6318	T6319	aux	to,bypass
R3884	T6269	T6271	npadvmod	catenin,conditional
R3885	T6270	T6269	punct	-,catenin
R3886	T6271	T6272	amod	conditional,targeting
R3887	T6319	T6301	xcomp	bypass,expected
R3888	T6272	T6267	pobj	targeting,by
R3889	T6273	T6245	punct	", ",allows
R389	T1192	T1191	punct	", ",growth
R3890	T6274	T6275	nsubj	Ajuba,interact
R3891	T6320	T6321	det	the,competition
R3892	T6275	T6245	ccomp	interact,allows
R3893	T6276	T6275	aux	to,interact
R3894	T6277	T6275	prep	with,interact
R3895	T6321	T6319	dobj	competition,bypass
R3896	T6278	T6277	pobj	Grb,with
R3897	T6279	T6278	punct	-,Grb
R3898	T6280	T6278	nummod	2,Grb
R3899	T6281	T6278	punct	/,Grb
R390	T1193	T1191	cc	and,growth
R3900	T6322	T6319	cc	and,bypass
R3901	T6282	T6278	appos	Sos,Grb
R3902	T6283	T6240	punct	.,demonstrate
R3903	T6323	T6319	conj	promote,bypass
R3904	T6285	T6286	mark	If,is
R3905	T6286	T6301	advcl	is,expected
R3906	T6287	T6288	det	the,competition
R3907	T6324	T6323	dobj	activation,promote
R3908	T6288	T6286	nsubj	competition,is
R3909	T6289	T6288	prep	between,competition
R391	T1194	T1191	conj	differentiation,growth
R3910	T6290	T6289	pobj	Grb,between
R3911	T6291	T6290	punct	-,Grb
R3912	T6292	T6290	nummod	2,Grb
R3913	T6293	T6290	punct	/,Grb
R3914	T6294	T6290	appos	Sos,Grb
R3915	T6325	T6324	prep	of,activation
R3916	T6295	T6290	cc	and,Grb
R3917	T6296	T6297	compound	α,catenin
R3918	T6297	T6290	conj	catenin,Grb
R3919	T6326	T6327	det	the,pathway
R392	T1195	T1196	punct	[,2
R3920	T6298	T6297	punct	-,catenin
R3921	T6299	T6288	prep	for,competition
R3922	T6300	T6299	pobj	Ajuba,for
R3923	T6327	T6325	pobj	pathway,of
R3924	T6302	T6303	advmod	functionally,relevant
R3925	T6328	T6329	compound	Ras,MAPK
R3926	T6329	T6327	compound	MAPK,pathway
R3927	T6330	T6329	punct	-,MAPK
R3928	T6331	T6324	prep	in,activation
R3929	T6409	T6407	oprd	inh,marked
R393	T1196	T1166	parataxis	2,trigger
R3930	T6410	T6405	punct	”,see
R3931	T6332	T6333	compound	WT,keratinocytes
R3932	T6411	T6402	punct	),4F
R3933	T6412	T6413	punct	[,27
R3934	T6413	T6380	parataxis	27,abolished
R3935	T6333	T6331	pobj	keratinocytes,in
R3936	T6414	T6413	punct	],27
R3937	T6415	T6380	punct	.,abolished
R3938	T6334	T6301	punct	.,expected
R3939	T6417	T6418	poss	Ajuba,domain
R394	T1197	T1196	punct	],2
R3940	T6418	T6421	nsubj	domain,is
R3941	T6419	T6417	case	's,Ajuba
R3942	T6336	T6337	advmod	Indeed,were
R3943	T6420	T6418	amod	pre-LIM,domain
R3944	T6422	T6423	det	the,segment
R3945	T6423	T6421	attr	segment,is
R3946	T6338	T6337	punct	", ",were
R3947	T6424	T6425	dep	that,associates
R3948	T6339	T6340	advmod	when,transfected
R3949	T6425	T6423	relcl	associates,segment
R395	T1198	T1166	punct	.,trigger
R3950	T6426	T6425	prep	with,associates
R3951	T6340	T6337	advcl	transfected,were
R3952	T6427	T6428	poss	Grb,domain
R3953	T6428	T6426	pobj	domain,with
R3954	T6429	T6427	punct	-,Grb
R3955	T6341	T6342	npadvmod	serum,starved
R3956	T6430	T6427	nummod	2,Grb
R3957	T6431	T6427	case	's,Grb
R3958	T6432	T6433	nmod	Src,homology
R3959	T6433	T6428	nmod	homology,domain
R396	T1200	T1201	advmod	How,collaborates
R3960	T6342	T6344	amod	starved,keratinocytes
R3961	T6434	T6433	punct	-,homology
R3962	T6435	T6433	nummod	3,homology
R3963	T6436	T6437	punct	[,9
R3964	T6437	T6421	parataxis	9,is
R3965	T6438	T6437	punct	],9
R3966	T6439	T6421	punct	.,is
R3967	T6343	T6342	punct	-,starved
R3968	T6441	T6442	advmod	When,overexpressed
R3969	T6442	T6446	advcl	overexpressed,observed
R397	T1201	T1206	csubj	collaborates,has
R3970	T6344	T6340	nsubjpass	keratinocytes,transfected
R3971	T6443	T6444	det	this,domain
R3972	T6444	T6442	nsubjpass	domain,overexpressed
R3973	T6445	T6442	auxpass	was,overexpressed
R3974	T6345	T6340	auxpass	were,transfected
R3975	T6447	T6442	prep	in,overexpressed
R3976	T6448	T6449	npadvmod	serum,starved
R3977	T6346	T6340	advmod	transiently,transfected
R3978	T6449	T6451	amod	starved,keratinocytes
R3979	T6450	T6449	punct	-,starved
R398	T1202	T1203	det	this,constellation
R3980	T6451	T6447	pobj	keratinocytes,in
R3981	T6347	T6340	prep	with,transfected
R3982	T6452	T6446	punct	", ",observed
R3983	T6453	T6454	det	a,elevation
R3984	T6454	T6446	nsubjpass	elevation,observed
R3985	T6348	T6349	det	an,vector
R3986	T6455	T6454	amod	comparable,elevation
R3987	T6456	T6454	prep	in,elevation
R3988	T6457	T6456	pobj	pMAPK,in
R3989	T6458	T6446	auxpass	was,observed
R399	T1203	T1201	nsubj	constellation,collaborates
R3990	T6349	T6347	pobj	vector,with
R3991	T6459	T6460	punct	(,4F
R3992	T6460	T6446	parataxis	4F,observed
R3993	T6461	T6460	compound	Figure,4F
R3994	T6350	T6351	compound	Ajuba,expression
R3995	T6462	T6460	punct	),4F
R3996	T6463	T6446	punct	.,observed
R3997	T6351	T6349	compound	expression,vector
R3998	T6465	T6466	mark	As,expected
R3999	T6466	T6467	advcl	expected,blocked
R400	T1204	T1203	prep	of,constellation
R4000	T6352	T6337	punct	", ",were
R4001	T6468	T6467	punct	", ",blocked
R4002	T6469	T6470	det	the,inhibitor
R4003	T6470	T6467	nsubj	inhibitor,blocked
R4004	T6471	T6470	amod	small,inhibitor
R4005	T6353	T6354	det	the,levels
R4006	T6472	T6470	compound	peptide,inhibitor
R4007	T6473	T6474	dep	that,interrupts
R4008	T6474	T6470	relcl	interrupts,inhibitor
R4009	T6354	T6337	nsubj	levels,were
R401	T1205	T1204	pobj	signals,of
R4010	T6475	T6476	det	the,association
R4011	T6476	T6474	dobj	association,interrupts
R4012	T6477	T6476	nmod	Grb,association
R4013	T6478	T6477	punct	-,Grb
R4014	T6479	T6477	nummod	2,Grb
R4015	T6355	T6354	prep	of,levels
R4016	T6480	T6477	punct	/,Grb
R4017	T6481	T6477	appos	Sos,Grb
R4018	T6482	T6483	det	the,effects
R4019	T6483	T6467	dobj	effects,blocked
R402	T1207	T1201	prep	in,collaborates
R4020	T6356	T6355	pobj	pMAPK,of
R4021	T6484	T6467	punct	.,blocked
R4022	T6486	T6487	det	These,data
R4023	T6357	T6358	preconj	not,elevated
R4024	T6487	T6488	nsubj	data,suggested
R4025	T6489	T6490	mark	that,associate
R4026	T6358	T6337	acomp	elevated,were
R4027	T6490	T6488	ccomp	associate,suggested
R4028	T6491	T6490	prep	by,associate
R4029	T6492	T6491	pcomp	elevating,by
R403	T1208	T1207	pcomp	tailoring,in
R4030	T6493	T6494	amod	cytosolic,levels
R4031	T6359	T6357	advmod	only,not
R4032	T6494	T6492	dobj	levels,elevating
R4033	T6495	T6494	compound	Ajuba,levels
R4034	T6496	T6490	punct	", ",associate
R4035	T6360	T6358	cc	but,elevated
R4036	T6497	T6498	poss	Ajuba,domain
R4037	T6498	T6490	nsubj	domain,associate
R4038	T6499	T6497	case	's,Ajuba
R4039	T6500	T6498	amod	pre-LIM,domain
R404	T1209	T1210	det	each,process
R4040	T6361	T6360	advmod	also,but
R4041	T6501	T6490	aux	may,associate
R4042	T6502	T6490	prep	with,associate
R4043	T6503	T6502	pobj	Grb,with
R4044	T6362	T6358	conj	comparable,elevated
R4045	T6504	T6503	punct	-,Grb
R4046	T6505	T6503	nummod	2,Grb
R4047	T6506	T6503	punct	/,Grb
R4048	T6363	T6362	prep	to,comparable
R4049	T6507	T6503	appos	Sos,Grb
R405	T1210	T1208	dobj	process,tailoring
R4050	T6508	T6490	prep	in,associate
R4051	T6509	T6510	det	a,manner
R4052	T6364	T6363	pobj	those,to
R4053	T6510	T6508	pobj	manner,in
R4054	T6511	T6512	dep	that,stimulates
R4055	T6365	T6364	acl	transfected,those
R4056	T6512	T6510	relcl	stimulates,manner
R4057	T6513	T6514	poss	its,activity
R4058	T6366	T6365	prep	with,transfected
R4059	T6367	T6368	det	the,transgene
R406	T1211	T1210	compound	budding,process
R4060	T6368	T6366	pobj	transgene,with
R4061	T6514	T6512	dobj	activity,stimulates
R4062	T6515	T6514	compound	nucleotide,activity
R4063	T6516	T6514	compound	exchange,activity
R4064	T6369	T6370	compound	K14,HASnail
R4065	T6517	T6512	cc	and,stimulates
R4066	T6518	T6512	conj	leads,stimulates
R4067	T6370	T6368	compound	HASnail,transgene
R4068	T6519	T6518	prep	to,leads
R4069	T6520	T6519	pobj	activation,to
R407	T1212	T1213	mark	so,executes
R4070	T6521	T6520	prep	of,activation
R4071	T6371	T6370	punct	-,HASnail
R4072	T6522	T6523	det	the,pathway
R4073	T6523	T6521	pobj	pathway,of
R4074	T6524	T6525	compound	Ras,MAPK
R4075	T6372	T6373	punct	(,4F
R4076	T6525	T6523	compound	MAPK,pathway
R4077	T6526	T6525	punct	-,MAPK
R4078	T6527	T6488	punct	.,suggested
R4079	T6373	T6337	parataxis	4F,were
R408	T1213	T1208	advcl	executes,tailoring
R4080	T6529	T6530	mark	Although,provides
R4081	T6530	T6533	advcl	provides,known
R4082	T6374	T6373	compound	Figure,4F
R4083	T6531	T6532	det	this,pathway
R4084	T6532	T6530	nsubj	pathway,provides
R4085	T6375	T6373	punct	),4F
R4086	T6534	T6535	nummod	one,mechanism
R4087	T6535	T6530	dobj	mechanism,provides
R4088	T6536	T6537	prep	by,coupled
R4089	T6537	T6535	relcl	coupled,mechanism
R409	T1214	T1213	mark	that,executes
R4090	T6376	T6337	punct	.,were
R4091	T6538	T6536	pobj	which,by
R4092	T6539	T6540	compound	Snail,expression
R4093	T6540	T6537	nsubjpass	expression,coupled
R4094	T6378	T6379	det	This,activation
R4095	T6541	T6540	cc	and,expression
R4096	T6542	T6540	conj	proliferation,expression
R4097	T6543	T6537	aux	may,coupled
R4098	T6544	T6537	auxpass	be,coupled
R4099	T6379	T6380	nsubjpass	activation,abolished
R410	T1215	T1213	nsubj	it,executes
R4100	T6545	T6537	prep	in,coupled
R4101	T6546	T6547	compound	skin,epithelium
R4102	T6547	T6545	pobj	epithelium,in
R4103	T6381	T6380	auxpass	was,abolished
R4104	T6548	T6533	punct	", ",known
R4105	T6549	T6550	amod	proliferative,circuitries
R4106	T6550	T6533	nsubjpass	circuitries,known
R4107	T6551	T6550	acl	involving,circuitries
R4108	T6382	T6383	advmod	when,treated
R4109	T6552	T6551	dobj	AJs,involving
R411	T1216	T1217	det	a,program
R4110	T6553	T6533	auxpass	are,known
R4111	T6554	T6555	aux	to,be
R4112	T6555	T6533	xcomp	be,known
R4113	T6383	T6380	advcl	treated,abolished
R4114	T6556	T6555	acomp	complex,be
R4115	T6557	T6556	cc	and,complex
R4116	T6558	T6559	advmod	often,interwoven
R4117	T6559	T6556	conj	interwoven,complex
R4118	T6560	T6533	punct	.,known
R4119	T6384	T6383	nsubjpass	cells,treated
R412	T1217	T1213	dobj	program,executes
R4120	T6562	T6563	amod	Future,studies
R4121	T6563	T6564	nsubjpass	studies,needed
R4122	T6385	T6383	auxpass	were,treated
R4123	T6565	T6564	aux	will,needed
R4124	T6566	T6564	auxpass	be,needed
R4125	T6567	T6568	aux	to,dissect
R4126	T6386	T6383	prep	with,treated
R4127	T6568	T6564	advcl	dissect,needed
R4128	T6569	T6568	advmod	systematically,dissect
R4129	T6570	T6571	det	these,intricacies
R413	T1218	T1217	amod	distinct,program
R4130	T6387	T6388	det	a,inhibitor
R4131	T6571	T6568	dobj	intricacies,dissect
R4132	T6572	T6571	amod	putative,intricacies
R4133	T6573	T6568	prep	at,dissect
R4134	T6388	T6386	pobj	inhibitor,with
R4135	T6574	T6575	det	a,level
R4136	T6575	T6573	pobj	level,at
R4137	T6576	T6575	amod	molecular,level
R4138	T6389	T6388	amod	small,inhibitor
R4139	T6577	T6564	punct	.,needed
R414	T1219	T1217	amod	morphogenetic,program
R4140	T6390	T6388	compound	peptide,inhibitor
R4141	T6391	T6392	dep	that,interrupts
R4142	T6392	T6388	relcl	interrupts,inhibitor
R4143	T6393	T6392	advmod	specifically,interrupts
R4144	T6394	T6395	det	the,interaction
R4145	T6395	T6392	dobj	interaction,interrupts
R4146	T6396	T6395	nmod	Grb,interaction
R4147	T6397	T6396	punct	-,Grb
R4148	T6398	T6396	nummod	2,Grb
R4149	T6399	T6396	punct	/,Grb
R415	T1220	T1221	advmod	yet,defined
R4150	T6400	T6396	appos	Sos,Grb
R4151	T6401	T6402	punct	(,4F
R4152	T6402	T6380	parataxis	4F,abolished
R4153	T6403	T6402	compound	Figure,4F
R4154	T6404	T6402	punct	;,4F
R4155	T6405	T6402	advcl	see,4F
R4156	T6406	T6405	dobj	lanes,see
R4157	T6407	T6406	acl	marked,lanes
R4158	T6408	T6409	punct	“,inh
R416	T1221	T1206	xcomp	defined,has
R417	T1222	T1221	aux	to,defined
R4172	T7354	T7355	csubj	Probing,Reveals
R4173	T7356	T7357	det	the,Regulation
R4174	T7357	T7354	dobj	Regulation,Probing
R4175	T7358	T7357	prep	of,Regulation
R4176	T7359	T7360	compound	Snail,Gene
R4177	T7360	T7361	compound	Gene,Expression
R4178	T7361	T7358	pobj	Expression,of
R4179	T7362	T7363	det	an,Link
R418	T1223	T1221	auxpass	be,defined
R4180	T7363	T7355	dobj	Link,Reveals
R4181	T7364	T7363	amod	Essential,Link
R4182	T7365	T7363	prep	to,Link
R4183	T7366	T7367	compound	TGF,β2
R4184	T7367	T7369	compound	β2,Signaling
R4185	T7368	T7367	punct	-,β2
R4186	T7369	T7365	pobj	Signaling,to
R4187	T7370	T7355	prep	in,Reveals
R4188	T7371	T7372	det	the,Bud
R4189	T7372	T7370	pobj	Bud,in
R419	T1224	T1221	advmod	comprehensively,defined
R4190	T7373	T7372	amod	Developing,Bud
R4191	T7374	T7372	compound	Hair,Bud
R4192	T7376	T7377	det	The,spike
R4193	T7377	T7379	nsubj	spike,prompted
R4194	T7378	T7377	amod	temporal,spike
R4195	T7380	T7377	prep	of,spike
R4196	T7381	T7382	compound	Snail,expression
R4197	T7382	T7380	pobj	expression,of
R4198	T7383	T7382	compound	mRNA,expression
R4199	T7384	T7377	prep	in,spike
R420	T1225	T1206	punct	.,has
R4200	T7385	T7386	det	the,bud
R4201	T7386	T7384	pobj	bud,in
R4202	T7387	T7386	compound	hair,bud
R4203	T7388	T7379	dobj	us,prompted
R4204	T7389	T7390	aux	to,consider
R4205	T7390	T7379	xcomp	consider,prompted
R4206	T7391	T7392	det	what,factor
R4207	T7392	T7393	dep	factor,regulating
R4208	T7393	T7390	ccomp	regulating,consider
R4209	T7394	T7392	punct	(,factor
R421	T1227	T1228	advmod	However,appears
R4210	T7395	T7392	nmod	s,factor
R4211	T7396	T7392	punct	),factor
R4212	T7397	T7393	aux	may,regulating
R4213	T7398	T7393	aux	be,regulating
R4214	T7399	T7400	det	the,gene
R4215	T7400	T7393	dobj	gene,regulating
R4216	T7401	T7400	compound	Snail,gene
R4217	T7402	T7379	punct	.,prompted
R4218	T7404	T7405	det	A,variety
R4219	T7405	T7406	nsubj	variety,have
R422	T1229	T1228	punct	", ",appears
R4220	T7407	T7405	prep	of,variety
R4221	T7408	T7409	amod	extracellular,signals
R4222	T7409	T7407	pobj	signals,of
R4223	T7410	T7411	det	an,impact
R4224	T7411	T7406	dobj	impact,have
R4225	T7412	T7411	prep	on,impact
R4226	T7413	T7414	det	the,expression
R4227	T7414	T7412	pobj	expression,on
R4228	T7415	T7416	compound	cell,type
R4229	T7416	T7417	npadvmod	type,specific
R423	T1230	T1231	det	the,process
R4230	T7417	T7414	amod	specific,expression
R4231	T7418	T7417	punct	-,specific
R4232	T7419	T7414	prep	of,expression
R4233	T7420	T7421	amod	different,members
R4234	T7421	T7419	pobj	members,of
R4235	T7422	T7423	compound	Snail,family
R4236	T7423	T7421	compound	family,members
R4237	T7424	T7406	punct	", ",have
R4238	T7425	T7406	cc	and,have
R4239	T7426	T7427	nsubj	many,affect
R424	T1231	T1228	nsubj	process,appears
R4240	T7427	T7406	conj	affect,have
R4241	T7428	T7426	prep	of,many
R4242	T7429	T7428	pobj	them,of
R4243	T7430	T7426	punct	", ",many
R4244	T7431	T7426	prep	including,many
R4245	T7432	T7431	pobj	Wnts,including
R4246	T7433	T7432	punct	", ",Wnts
R4247	T7434	T7432	conj	BMPs,Wnts
R4248	T7435	T7434	punct	", ",BMPs
R4249	T7436	T7434	conj	FGFs,BMPs
R425	T1232	T1233	aux	to,patterned
R4250	T7437	T7436	punct	", ",FGFs
R4251	T7438	T7436	cc	and,FGFs
R4252	T7439	T7440	compound	TGF,βs
R4253	T7440	T7436	conj	βs,FGFs
R4254	T7441	T7440	punct	-,βs
R4255	T7442	T7427	punct	", ",affect
R4256	T7443	T7427	advmod	also,affect
R4257	T7444	T7445	compound	hair,bud
R4258	T7445	T7446	compound	bud,development
R4259	T7446	T7427	dobj	development,affect
R426	T1233	T1228	xcomp	patterned,appears
R4260	T7447	T7448	punct	[,28
R4261	T7448	T7427	parataxis	28,affect
R4262	T7449	T7448	nummod	2,28
R4263	T7450	T7448	punct	",",28
R4264	T7451	T7448	nummod	16,28
R4265	T7452	T7448	punct	",",28
R4266	T7453	T7448	punct	],28
R4267	T7454	T7427	punct	.,affect
R4268	T7456	T7457	mark	Since,expressed
R4269	T7457	T7461	advcl	expressed,focused
R427	T1234	T1233	auxpass	be,patterned
R4270	T7458	T7457	nsubjpass	Snail,expressed
R4271	T7459	T7457	auxpass	is,expressed
R4272	T7460	T7457	neg	not,expressed
R4273	T7462	T7457	prep	in,expressed
R4274	T7463	T7464	amod	cultured,keratinocytes
R4275	T7464	T7462	pobj	keratinocytes,in
R4276	T7465	T7464	compound	skin,keratinocytes
R4277	T7466	T7467	dep	that,secrete
R4278	T7467	T7464	relcl	secrete,keratinocytes
R4279	T7468	T7469	amod	active,BMPs
R428	T1235	T1233	prep	at,patterned
R4280	T7469	T7467	dobj	BMPs,secrete
R4281	T7470	T7469	cc	and,BMPs
R4282	T7471	T7469	conj	FGFs,BMPs
R4283	T7472	T7473	punct	(,see
R4284	T7473	T7457	parataxis	see,expressed
R4285	T7474	T7475	compound	Figure,1B
R4286	T7475	T7473	dobj	1B,see
R4287	T7476	T7473	punct	),see
R4288	T7477	T7461	punct	", ",focused
R4289	T7478	T7461	nsubj	we,focused
R429	T1236	T1237	det	the,stages
R4290	T7479	T7480	poss	our,attention
R4291	T7480	T7461	dobj	attention,focused
R4292	T7481	T7461	prep	on,focused
R4293	T7482	T7483	nmod	Wnt,signaling
R4294	T7483	T7481	pobj	signaling,on
R4295	T7484	T7482	cc	and,Wnt
R4296	T7485	T7486	compound	TGF,β
R4297	T7486	T7482	conj	β,Wnt
R4298	T7487	T7486	punct	-,β
R4299	T7488	T7483	prep	as,signaling
R430	T1237	T1235	pobj	stages,at
R4300	T7489	T7490	advmod	more,likely
R4301	T7490	T7491	amod	likely,candidates
R4302	T7491	T7488	pobj	candidates,as
R4303	T7492	T7491	prep	for,candidates
R4304	T7493	T7494	compound	Snail,induction
R4305	T7494	T7492	pobj	induction,for
R4306	T7495	T7491	prep	in,candidates
R4307	T7496	T7497	det	this,type
R4308	T7497	T7495	pobj	type,in
R4309	T7498	T7497	compound	cell,type
R431	T1238	T1237	amod	initial,stages
R4310	T7499	T7461	punct	.,focused
R4311	T7501	T7502	advmod	Previously,showed
R4312	T7503	T7502	punct	", ",showed
R4313	T7504	T7502	nsubj	we,showed
R4314	T7505	T7506	mark	that,achieved
R4315	T7506	T7502	ccomp	achieved,showed
R4316	T7507	T7508	amod	effective,transmission
R4317	T7508	T7506	nsubjpass	transmission,achieved
R4318	T7509	T7508	prep	of,transmission
R4319	T7510	T7511	det	a,signal
R432	T1239	T1237	prep	of,stages
R4320	T7511	T7509	pobj	signal,of
R4321	T7512	T7511	nmod	Wnt,signal
R4322	T7513	T7512	punct	-,Wnt
R4323	T7514	T7512	nummod	3a,Wnt
R4324	T7515	T7508	prep	in,transmission
R4325	T7516	T7517	amod	cultured,keratinocytes
R4326	T7517	T7515	pobj	keratinocytes,in
R4327	T7518	T7506	aux	can,achieved
R4328	T7519	T7506	auxpass	be,achieved
R4329	T7520	T7506	prep	through,achieved
R433	T1240	T1241	compound	bud,formation
R4330	T7521	T7522	poss	their,exposure
R4331	T7522	T7520	pobj	exposure,through
R4332	T7523	T7522	prep	to,exposure
R4333	T7524	T7525	det	the,inhibitor
R4334	T7525	T7523	pobj	inhibitor,to
R4335	T7526	T7525	compound	BMP,inhibitor
R4336	T7527	T7525	appos	noggin,inhibitor
R4337	T7528	T7525	punct	", ",inhibitor
R4338	T7529	T7530	dep	which,induces
R4339	T7530	T7525	relcl	induces,inhibitor
R434	T1241	T1239	pobj	formation,of
R4340	T7531	T7532	nmod	LEF,expression
R4341	T7532	T7530	dobj	expression,induces
R4342	T7533	T7531	punct	-,LEF
R4343	T7534	T7531	nummod	1,LEF
R4344	T7535	T7536	punct	[,4
R4345	T7536	T7502	parataxis	4,showed
R4346	T7537	T7536	punct	],4
R4347	T7538	T7502	punct	.,showed
R4348	T7540	T7541	advmod	In,vitro
R4349	T7541	T7542	advmod	vitro,regulated
R435	T1242	T1228	punct	", ",appears
R4350	T7543	T7542	punct	", ",regulated
R4351	T7544	T7545	det	these,conditions
R4352	T7545	T7542	nsubj	conditions,regulated
R4353	T7546	T7542	advmod	down,regulated
R4354	T7547	T7542	punct	-,regulated
R4355	T7548	T7549	det	the,promoter
R4356	T7549	T7542	dobj	promoter,regulated
R4357	T7550	T7551	compound	E,cadherin
R4358	T7551	T7549	compound	cadherin,promoter
R4359	T7552	T7551	punct	-,cadherin
R436	T1243	T1244	mark	since,differ
R4360	T7553	T7542	cc	and,regulated
R4361	T7554	T7542	conj	induced,regulated
R4362	T7555	T7556	det	a,gene
R4363	T7556	T7554	dobj	gene,induced
R4364	T7557	T7558	npadvmod	LEF,sensitive
R4365	T7558	T7556	amod	sensitive,gene
R4366	T7559	T7557	punct	-,LEF
R4367	T7560	T7557	nummod	1,LEF
R4368	T7561	T7557	punct	/,LEF
R4369	T7562	T7563	compound	β,catenin
R437	T1244	T1228	advcl	differ,appears
R4370	T7563	T7557	appos	catenin,LEF
R4371	T7564	T7563	punct	-,catenin
R4372	T7565	T7558	punct	-,sensitive
R4373	T7566	T7556	compound	reporter,gene
R4374	T7567	T7556	punct	", ",gene
R4375	T7568	T7556	appos	TOPFLASH,gene
R4376	T7569	T7570	punct	[,4
R4377	T7570	T7554	parataxis	4,induced
R4378	T7571	T7570	punct	],4
R4379	T7572	T7542	punct	.,regulated
R438	T1245	T1246	det	the,importance
R4380	T7574	T7575	prep	In,induced
R4381	T7576	T7574	pobj	contrast,In
R4382	T7577	T7575	punct	", ",induced
R4383	T7578	T7579	compound	Snail,expression
R4384	T7579	T7575	nsubjpass	expression,induced
R4385	T7580	T7575	auxpass	was,induced
R4386	T7581	T7575	neg	not,induced
R4387	T7582	T7575	agent	by,induced
R4388	T7583	T7584	det	these,conditions
R4389	T7584	T7582	pobj	conditions,by
R439	T1246	T1244	nsubj	importance,differ
R4390	T7585	T7586	punct	(,5A
R4391	T7586	T7575	parataxis	5A,induced
R4392	T7587	T7586	compound	Figure,5A
R4393	T7588	T7586	punct	),5A
R4394	T7589	T7575	punct	.,induced
R4395	T7591	T7592	advmod	Thus,support
R4396	T7593	T7592	punct	", ",support
R4397	T7594	T7592	prep	despite,support
R4398	T7595	T7596	amod	essential,roles
R4399	T7596	T7594	pobj	roles,despite
R440	T1247	T1246	amod	relative,importance
R4400	T7597	T7596	prep	for,roles
R4401	T7598	T7597	pobj	Wnts,for
R4402	T7599	T7598	cc	and,Wnts
R4403	T7600	T7598	conj	noggin,Wnts
R4404	T7601	T7596	prep	in,roles
R4405	T7602	T7603	compound	hair,follicle
R4406	T7603	T7604	compound	follicle,specification
R4407	T7604	T7601	pobj	specification,in
R4408	T7605	T7606	punct	[,30
R4409	T7606	T7596	parataxis	30,roles
R441	T1248	T1246	prep	of,importance
R4410	T7607	T7606	nummod	4,30
R4411	T7608	T7606	punct	",",30
R4412	T7609	T7606	nummod	29,30
R4413	T7610	T7606	punct	",",30
R4414	T7611	T7606	punct	],30
R4415	T7612	T7592	punct	", ",support
R4416	T7613	T7614	poss	our,studies
R4417	T7614	T7592	nsubj	studies,support
R4418	T7615	T7592	aux	did,support
R4419	T7616	T7592	neg	not,support
R442	T1249	T1250	det	these,pathways
R4420	T7617	T7618	det	an,role
R4421	T7618	T7592	dobj	role,support
R4422	T7619	T7618	amod	essential,role
R4423	T7620	T7618	prep	for,role
R4424	T7621	T7622	det	these,signals
R4425	T7622	T7620	pobj	signals,for
R4426	T7623	T7618	prep	in,role
R4427	T7624	T7623	pcomp	governing,in
R4428	T7625	T7626	compound	Snail,expression
R4429	T7626	T7624	dobj	expression,governing
R443	T1250	T1248	pobj	pathways,of
R4430	T7627	T7624	prep	in,governing
R4431	T7628	T7627	pobj	keratinocytes,in
R4432	T7629	T7592	punct	.,support
R4433	T7631	T7632	compound	TGF,β1
R4434	T7632	T7634	nsubjpass	β1,shown
R4435	T7633	T7632	punct	-,β1
R4436	T7635	T7634	aux	has,shown
R4437	T7636	T7634	auxpass	been,shown
R4438	T7637	T7638	aux	to,induce
R4439	T7638	T7634	xcomp	induce,shown
R444	T1251	T1250	cc	and,pathways
R4440	T7639	T7640	compound	Snail,members
R4441	T7640	T7638	dobj	members,induce
R4442	T7641	T7640	compound	family,members
R4443	T7642	T7638	prep	in,induce
R4444	T7643	T7642	pobj	hepatocytes,in
R4445	T7644	T7643	cc	and,hepatocytes
R4446	T7645	T7643	conj	heart,hepatocytes
R4447	T7646	T7647	punct	[,31
R4448	T7647	T7634	parataxis	31,shown
R4449	T7648	T7647	nummod	15,31
R445	T1252	T1253	poss	their,effectors
R4450	T7649	T7647	punct	", ",31
R4451	T7650	T7647	punct	],31
R4452	T7651	T7634	punct	.,shown
R4453	T7653	T7654	prep	In,inhibits
R4454	T7655	T7653	pobj	keratinocytes,In
R4455	T7656	T7654	punct	", ",inhibits
R4456	T7657	T7654	advmod	however,inhibits
R4457	T7658	T7654	punct	", ",inhibits
R4458	T7659	T7660	compound	TGF,β1
R4459	T7660	T7654	nsubj	β1,inhibits
R446	T1253	T1250	conj	effectors,pathways
R4460	T7661	T7660	punct	-,β1
R4461	T7662	T7663	compound	keratinocyte,growth
R4462	T7663	T7654	dobj	growth,inhibits
R4463	T7664	T7654	cc	and,inhibits
R4464	T7665	T7654	conj	seems,inhibits
R4465	T7666	T7667	aux	to,involved
R4466	T7667	T7665	xcomp	involved,seems
R4467	T7668	T7667	auxpass	be,involved
R4468	T7669	T7667	prep	in,involved
R4469	T7670	T7669	pcomp	triggering,in
R447	T1254	T1253	amod	downstream,effectors
R4470	T7671	T7672	det	the,phase
R4471	T7672	T7670	dobj	phase,triggering
R4472	T7673	T7672	amod	destructive,phase
R4473	T7674	T7672	prep	of,phase
R4474	T7675	T7676	det	the,follicle
R4475	T7676	T7674	pobj	follicle,of
R4476	T7677	T7676	amod	cycling,follicle
R4477	T7678	T7676	compound	hair,follicle
R4478	T7679	T7680	punct	[,32
R4479	T7680	T7665	parataxis	32,seems
R448	T1255	T1256	mark	as,begin
R4480	T7681	T7680	punct	],32
R4481	T7682	T7654	punct	.,inhibits
R4482	T7684	T7685	prep	Of,blocked
R4483	T7686	T7687	det	the,mutations
R4484	T7687	T7684	pobj	mutations,Of
R4485	T7688	T7687	nmod	loss,mutations
R4486	T7689	T7688	punct	-,loss
R4487	T7690	T7688	prep	of,loss
R4488	T7691	T7690	punct	-,of
R4489	T7692	T7690	pobj	function,of
R449	T1256	T1244	advcl	begin,differ
R4490	T7693	T7687	acl	generated,mutations
R4491	T7694	T7693	prep	in,generated
R4492	T7695	T7694	pobj	each,in
R4493	T7696	T7695	prep	of,each
R4494	T7697	T7698	det	the,genes
R4495	T7698	T7696	pobj	genes,of
R4496	T7699	T7700	compound	TGF,β
R4497	T7700	T7698	compound	β,genes
R4498	T7701	T7700	punct	-,β
R4499	T7702	T7685	punct	", ",blocked
R450	T1257	T1256	nsubj	buds,begin
R4500	T7703	T7704	advmod	only,state
R4501	T7704	T7685	nsubj	state,blocked
R4502	T7705	T7704	det	the,state
R4503	T7706	T7707	nmod	TGF,β2
R4504	T7707	T7704	nmod	β2,state
R4505	T7708	T7707	punct	-,β2
R4506	T7709	T7704	amod	null,state
R4507	T7710	T7711	compound	follicle,development
R4508	T7711	T7685	dobj	development,blocked
R4509	T7712	T7685	prep	at,blocked
R451	T1258	T1259	aux	to,develop
R4510	T7713	T7714	det	the,stage
R4511	T7714	T7712	pobj	stage,at
R4512	T7715	T7716	compound	hair,bud
R4513	T7716	T7714	compound	bud,stage
R4514	T7717	T7718	punct	[,32
R4515	T7718	T7685	parataxis	32,blocked
R4516	T7719	T7718	punct	],32
R4517	T7720	T7685	punct	.,blocked
R4518	T7722	T7723	advmod	Thus,turned
R4519	T7724	T7723	punct	", ",turned
R452	T1259	T1256	xcomp	develop,begin
R4520	T7725	T7723	nsubj	we,turned
R4521	T7726	T7723	prep	towards,turned
R4522	T7727	T7726	pcomp	addressing,towards
R4523	T7728	T7729	mark	whether,involved
R4524	T7729	T7727	ccomp	involved,addressing
R4525	T7730	T7731	compound	TGF,β2
R4526	T7731	T7729	nsubjpass	β2,involved
R4527	T7732	T7731	punct	-,β2
R4528	T7733	T7729	aux	might,involved
R4529	T7734	T7729	auxpass	be,involved
R453	T1260	T1256	cc	and,begin
R4530	T7735	T7729	prep	in,involved
R4531	T7736	T7735	pcomp	regulating,in
R4532	T7737	T7738	compound	Snail,expression
R4533	T7738	T7736	dobj	expression,regulating
R4534	T7739	T7736	prep	in,regulating
R4535	T7740	T7739	pobj	keratinocytes,in
R4536	T7741	T7740	acl	isolated,keratinocytes
R4537	T7742	T7741	prep	from,isolated
R4538	T7743	T7744	det	the,layer
R4539	T7744	T7742	pobj	layer,from
R454	T1261	T1262	compound	cell,fates
R4540	T7745	T7744	amod	basal,layer
R4541	T7746	T7744	prep	of,layer
R4542	T7747	T7748	det	the,epidermis
R4543	T7748	T7746	pobj	epidermis,of
R4544	T7749	T7723	punct	.,turned
R4545	T7751	T7752	mark	Though,is
R4546	T7752	T7754	advcl	is,are
R4547	T7753	T7752	expl	there,is
R4548	T7755	T7756	det	no,system
R4549	T7756	T7752	attr	system,is
R455	T1262	T1263	nsubjpass	fates,specified
R4550	T7757	T7758	compound	cell,culture
R4551	T7758	T7756	compound	culture,system
R4552	T7759	T7756	amod	available,system
R4553	T7760	T7761	aux	to,study
R4554	T7761	T7759	xcomp	study,available
R4555	T7762	T7761	advmod	specifically,study
R4556	T7763	T7764	amod	placodal,cells
R4557	T7764	T7761	dobj	cells,study
R4558	T7765	T7754	punct	", ",are
R4559	T7766	T7767	det	these,keratinocytes
R456	T1263	T1256	conj	specified,begin
R4560	T7767	T7754	nsubj	keratinocytes,are
R4561	T7768	T7769	poss	their,progenitors
R4562	T7769	T7754	attr	progenitors,are
R4563	T7770	T7754	cc	and,are
R4564	T7771	T7754	conj	are,are
R4565	T7772	T7773	det	the,approximation
R4566	T7773	T7771	attr	approximation,are
R4567	T7774	T7773	amod	closest,approximation
R4568	T7775	T7773	amod	available,approximation
R4569	T7776	T7777	aux	to,study
R457	T1264	T1263	auxpass	are,specified
R4570	T7777	T7775	xcomp	study,available
R4571	T7778	T7779	det	the,behavior
R4572	T7779	T7777	dobj	behavior,study
R4573	T7780	T7779	prep	of,behavior
R4574	T7781	T7782	amod	epithelial,cells
R4575	T7782	T7780	pobj	cells,of
R4576	T7783	T7782	prep	of,cells
R4577	T7784	T7785	det	the,placode
R4578	T7785	T7783	pobj	placode,of
R4579	T7786	T7754	punct	.,are
R458	T1265	T1228	punct	.,appears
R4580	T7788	T7789	advmod	Interestingly,caused
R4581	T7790	T7789	punct	", ",caused
R4582	T7791	T7789	nsubj	treatment,caused
R4583	T7792	T7791	prep	of,treatment
R4584	T7793	T7794	amod	cultured,keratinocytes
R4585	T7794	T7792	pobj	keratinocytes,of
R4586	T7795	T7791	prep	with,treatment
R4587	T7796	T7797	advmod	as,5
R4588	T7797	T7800	nummod	5,ng
R4589	T7798	T7797	amod	little,5
R459	T1267	T1268	det	The,development
R4590	T7799	T7797	quantmod	as,5
R4591	T7800	T7795	pobj	ng,with
R4592	T7801	T7802	punct	/,ml
R4593	T7802	T7800	prep	ml,ng
R4594	T7803	T7800	prep	of,ng
R4595	T7804	T7805	compound	TGF,β2
R4596	T7805	T7803	pobj	β2,of
R4597	T7806	T7805	punct	-,β2
R4598	T7807	T7808	det	a,induction
R4599	T7808	T7789	dobj	induction,caused
R460	T1268	T1269	nsubj	development,requires
R4600	T7809	T7808	amod	rapid,induction
R4601	T7810	T7809	cc	and,rapid
R4602	T7811	T7809	conj	transient,rapid
R4603	T7812	T7808	prep	of,induction
R4604	T7813	T7812	pobj	Snail,of
R4605	T7814	T7815	punct	(,5B
R4606	T7815	T7789	parataxis	5B,caused
R4607	T7816	T7815	compound	Figure,5B
R4608	T7817	T7815	punct	),5B
R4609	T7818	T7789	punct	.,caused
R461	T1270	T1268	prep	of,development
R4610	T7820	T7821	prep	Following,detected
R4611	T7822	T7823	det	this,treatment
R4612	T7823	T7820	pobj	treatment,Following
R4613	T7824	T7821	punct	", ",detected
R4614	T7825	T7826	compound	Snail,protein
R4615	T7826	T7821	nsubjpass	protein,detected
R4616	T7827	T7821	auxpass	was,detected
R4617	T7828	T7821	prep	within,detected
R4618	T7829	T7830	nummod	30,min
R4619	T7830	T7828	pobj	min,within
R462	T1271	T1272	det	a,bud
R4620	T7831	T7821	punct	", ",detected
R4621	T7832	T7821	conj	peaked,detected
R4622	T7833	T7832	prep	at,peaked
R4623	T7834	T7835	nummod	2,h
R4624	T7835	T7833	pobj	h,at
R4625	T7836	T7832	punct	", ",peaked
R4626	T7837	T7832	cc	and,peaked
R4627	T7838	T7839	advmod	then,declined
R4628	T7839	T7832	conj	declined,peaked
R4629	T7840	T7839	advmod	thereafter,declined
R463	T1272	T1270	pobj	bud,of
R4630	T7841	T7821	punct	.,detected
R4631	T7843	T7844	det	The,induction
R4632	T7844	T7845	nsubj	induction,appeared
R4633	T7846	T7844	prep	of,induction
R4634	T7847	T7846	pobj	Snail,of
R4635	T7848	T7849	aux	to,be
R4636	T7849	T7845	xcomp	be,appeared
R4637	T7850	T7849	acomp	specific,be
R4638	T7851	T7850	prep	for,specific
R4639	T7852	T7853	det	the,form
R464	T1273	T1274	det	a,number
R4640	T7853	T7851	pobj	form,for
R4641	T7854	T7853	amod	active,form
R4642	T7855	T7853	prep	of,form
R4643	T7856	T7857	det	the,factor
R4644	T7857	T7855	pobj	factor,of
R4645	T7858	T7857	compound	growth,factor
R4646	T7859	T7849	punct	", ",be
R4647	T7860	T7861	mark	as,obliterated
R4648	T7861	T7849	advcl	obliterated,be
R4649	T7862	T7861	nsubj	pretreatment,obliterated
R465	T1274	T1269	dobj	number,requires
R4650	T7863	T7862	prep	of,pretreatment
R4651	T7864	T7865	compound	TGF,β2
R4652	T7865	T7863	pobj	β2,of
R4653	T7866	T7865	punct	-,β2
R4654	T7867	T7862	prep	for,pretreatment
R4655	T7868	T7869	nummod	10,min
R4656	T7869	T7867	pobj	min,for
R4657	T7870	T7862	prep	at,pretreatment
R4658	T7871	T7872	nummod	100,°C
R4659	T7872	T7870	pobj	°C,at
R466	T1275	T1274	prep	of,number
R4660	T7873	T7874	det	the,response
R4661	T7874	T7861	dobj	response,obliterated
R4662	T7875	T7876	punct	[,lanes
R4663	T7876	T7849	parataxis	lanes,be
R4664	T7877	T7878	compound	Figure,5B
R4665	T7878	T7876	dep	5B,lanes
R4666	T7879	T7876	punct	", ",lanes
R4667	T7880	T7876	acl	marked,lanes
R4668	T7881	T7880	punct	(,marked
R4669	T7882	T7880	oprd	–,marked
R467	T1276	T1277	amod	coordinated,changes
R4670	T7883	T7876	punct	),lanes
R4671	T7884	T7876	punct	],lanes
R4672	T7885	T7845	punct	.,appeared
R4673	T7887	T7888	prep	By,maintained
R4674	T7889	T7887	pobj	contrast,By
R4675	T7890	T7888	punct	", ",maintained
R4676	T7891	T7892	mark	although,remained
R4677	T7892	T7888	advcl	remained,maintained
R4678	T7893	T7894	compound	TGF,β
R4679	T7894	T7896	compound	β,receptor
R468	T1277	T1275	pobj	changes,of
R4680	T7895	T7894	punct	-,β
R4681	T7896	T7897	compound	receptor,activation
R4682	T7897	T7892	nsubj	activation,remained
R4683	T7898	T7892	acomp	elevated,remained
R4684	T7899	T7892	prep	during,remained
R4685	T7900	T7901	det	the,duration
R4686	T7901	T7899	pobj	duration,during
R4687	T7902	T7901	prep	of,duration
R4688	T7903	T7904	det	the,experiment
R4689	T7904	T7902	pobj	experiment,of
R469	T1278	T1277	prep	in,changes
R4690	T7905	T7892	punct	(,remained
R4691	T7906	T7907	mark	as,measured
R4692	T7907	T7892	advcl	measured,remained
R4693	T7908	T7907	prep	by,measured
R4694	T7909	T7910	det	the,phosphorylation
R4695	T7910	T7908	pobj	phosphorylation,by
R4696	T7911	T7910	amod	sustained,phosphorylation
R4697	T7912	T7910	prep	of,phosphorylation
R4698	T7913	T7914	det	the,effector
R4699	T7914	T7912	pobj	effector,of
R470	T1279	T1280	det	the,behavior
R4700	T7915	T7914	amod	downstream,effector
R4701	T7916	T7914	appos	SMAD2,effector
R4702	T7917	T7888	punct	),maintained
R4703	T7918	T7919	compound	Snail,expression
R4704	T7919	T7888	nsubjpass	expression,maintained
R4705	T7920	T7888	aux	could,maintained
R4706	T7921	T7888	neg	not,maintained
R4707	T7922	T7888	auxpass	be,maintained
R4708	T7923	T7924	punct	(,5B
R4709	T7924	T7888	parataxis	5B,maintained
R471	T1280	T1278	pobj	behavior,in
R4710	T7925	T7924	compound	Figure,5B
R4711	T7926	T7924	punct	),5B
R4712	T7927	T7888	punct	.,maintained
R4713	T7929	T7930	advmod	Thus,seemed
R4714	T7931	T7930	punct	", ",seemed
R4715	T7932	T7933	mark	although,correlated
R4716	T7933	T7930	advcl	correlated,seemed
R4717	T7934	T7935	compound	Snail,expression
R4718	T7935	T7933	nsubj	expression,correlated
R4719	T7936	T7933	prep	with,correlated
R472	T1281	T1280	prep	of,behavior
R4720	T7937	T7938	amod	phosphorylated,SMAD2
R4721	T7938	T7939	nmod	SMAD2,induction
R4722	T7939	T7936	pobj	induction,with
R4723	T7940	T7938	punct	(,SMAD2
R4724	T7941	T7938	appos	pSMAD2,SMAD2
R4725	T7942	T7939	punct	),induction
R4726	T7943	T7930	punct	", ",seemed
R4727	T7944	T7945	poss	its,decline
R4728	T7945	T7930	nsubj	decline,seemed
R4729	T7946	T7947	aux	to,rely
R473	T1282	T1283	det	the,cells
R4730	T7947	T7930	xcomp	rely,seemed
R4731	T7948	T7947	prep	on,rely
R4732	T7949	T7950	amod	secondary,events
R4733	T7950	T7948	pobj	events,on
R4734	T7951	T7950	amod	downstream,events
R4735	T7952	T7930	punct	.,seemed
R4736	T7954	T7955	det	The,kinetics
R4737	T7955	T7957	nsubjpass	kinetics,reflected
R4738	T7956	T7955	amod	rapid,kinetics
R4739	T7958	T7955	prep	of,kinetics
R474	T1283	T1281	pobj	cells,of
R4740	T7959	T7960	compound	Snail,expression
R4741	T7960	T7958	pobj	expression,of
R4742	T7961	T7957	auxpass	were,reflected
R4743	T7962	T7957	prep	at,reflected
R4744	T7963	T7964	det	the,level
R4745	T7964	T7962	pobj	level,at
R4746	T7965	T7964	compound	mRNA,level
R4747	T7966	T7957	punct	", ",reflected
R4748	T7967	T7957	advcl	suggesting,reflected
R4749	T7968	T7969	mark	that,be
R475	T1284	T1283	amod	targeted,cells
R4750	T7969	T7967	ccomp	be,suggesting
R4751	T7970	T7971	compound	Snail,promoter
R4752	T7971	T7972	compound	promoter,activity
R4753	T7972	T7969	nsubj	activity,be
R4754	T7973	T7972	prep	in,activity
R4755	T7974	T7973	pobj	keratinocytes,in
R4756	T7975	T7969	aux	might,be
R4757	T7976	T7969	acomp	sensitive,be
R4758	T7977	T7976	prep	to,sensitive
R4759	T7978	T7979	compound	TGF,β2
R476	T1285	T1280	prep	within,behavior
R4760	T7979	T7981	compound	β2,signaling
R4761	T7980	T7979	punct	-,β2
R4762	T7981	T7977	pobj	signaling,to
R4763	T7982	T7983	punct	(,5C
R4764	T7983	T7957	parataxis	5C,reflected
R4765	T7984	T7983	compound	Figure,5C
R4766	T7985	T7983	punct	),5C
R4767	T7986	T7957	punct	.,reflected
R4768	T7988	T7989	aux	To,test
R4769	T7989	T7990	advcl	test,engineered
R477	T1286	T1287	det	an,sheet
R4770	T7991	T7992	det	this,possibility
R4771	T7992	T7989	dobj	possibility,test
R4772	T7993	T7990	punct	", ",engineered
R4773	T7994	T7990	nsubj	we,engineered
R4774	T7995	T7996	det	a,transgene
R4775	T7996	T7990	dobj	transgene,engineered
R4776	T7997	T7996	acl	driving,transgene
R4777	T7998	T7999	det	the,reporter
R4778	T7999	T7997	dobj	reporter,driving
R4779	T8000	T8001	compound	β,galactosidase
R478	T1287	T1285	pobj	sheet,within
R4780	T8001	T7999	compound	galactosidase,reporter
R4781	T8002	T8001	punct	-,galactosidase
R4782	T8003	T7997	prep	under,driving
R4783	T8004	T8005	det	the,control
R4784	T8005	T8003	pobj	control,under
R4785	T8006	T8005	prep	of,control
R4786	T8007	T8008	advmod	approximately,2.2
R4787	T8008	T8009	nummod	2.2,kb
R4788	T8009	T8006	pobj	kb,of
R4789	T8010	T8009	prep	of,kb
R479	T1288	T1287	amod	epithelial,sheet
R4790	T8011	T8012	compound	promoter,sequence
R4791	T8012	T8010	pobj	sequence,of
R4792	T8013	T8012	acl	located,sequence
R4793	T8014	T8013	npadvmod	5,located
R4794	T8015	T8014	punct	′,5
R4795	T8016	T8014	prep	from,5
R4796	T8017	T8018	det	the,site
R4797	T8018	T8016	pobj	site,from
R4798	T8019	T8018	compound	transcription,site
R4799	T8020	T8018	compound	initiation,site
R480	T1289	T1269	punct	.,requires
R4800	T8021	T8018	prep	of,site
R4801	T8022	T8023	det	the,gene
R4802	T8023	T8021	pobj	gene,of
R4803	T8024	T8023	compound	mouse,gene
R4804	T8025	T8023	compound	Snail,gene
R4805	T8026	T7990	punct	.,engineered
R4806	T8028	T8029	prep	At,treated
R4807	T8030	T8031	nummod	2,d
R4808	T8031	T8028	pobj	d,At
R4809	T8032	T8031	prep	after,d
R481	T1291	T1292	det	The,process
R4810	T8033	T8034	amod	transient,transfection
R4811	T8034	T8032	pobj	transfection,after
R4812	T8035	T8029	punct	", ",treated
R4813	T8036	T8029	nsubjpass	keratinocytes,treated
R4814	T8037	T8029	auxpass	were,treated
R4815	T8038	T8029	prep	with,treated
R4816	T8039	T8040	compound	TGF,β2
R4817	T8040	T8038	pobj	β2,with
R4818	T8041	T8040	punct	-,β2
R4819	T8042	T8043	punct	(,0
R482	T1292	T1293	nsubjpass	process,accompanied
R4820	T8043	T8029	parataxis	0,treated
R4821	T8044	T8043	nsubj	t,0
R4822	T8045	T8043	punct	=,0
R4823	T8046	T8043	punct	),0
R4824	T8047	T8029	cc	and,treated
R4825	T8048	T8049	advmod	then,assayed
R4826	T8049	T8029	conj	assayed,treated
R4827	T8050	T8049	prep	for,assayed
R4828	T8051	T8052	compound	transgene,activity
R4829	T8052	T8050	pobj	activity,for
R483	T1294	T1293	aux	must,accompanied
R4830	T8053	T8049	prep	over,assayed
R4831	T8054	T8055	det	the,course
R4832	T8055	T8053	pobj	course,over
R4833	T8056	T8055	amod	same,course
R4834	T8057	T8055	compound	time,course
R4835	T8058	T8059	prep	in,observed
R4836	T8059	T8055	relcl	observed,course
R4837	T8060	T8058	pobj	which,in
R4838	T8061	T8059	nsubj	we,observed
R4839	T8062	T8059	aux	had,observed
R484	T1295	T1293	auxpass	be,accompanied
R4840	T8063	T8064	compound	Snail,protein
R4841	T8064	T8065	compound	protein,induction
R4842	T8065	T8059	dobj	induction,observed
R4843	T8066	T8029	punct	.,treated
R4844	T8068	T8069	det	The,results
R4845	T8069	T8070	nsubjpass	results,presented
R4846	T8071	T8069	prep	of,results
R4847	T8072	T8073	det	this,experiment
R4848	T8073	T8071	pobj	experiment,of
R4849	T8074	T8070	auxpass	are,presented
R485	T1296	T1293	prep	by,accompanied
R4850	T8075	T8070	prep	in,presented
R4851	T8076	T8077	compound	Figure,5D
R4852	T8077	T8075	pobj	5D,in
R4853	T8078	T8070	punct	.,presented
R4854	T8080	T8081	prep	Within,increased
R4855	T8082	T8083	nummod	0.5,h
R4856	T8083	T8080	pobj	h,Within
R4857	T8084	T8083	prep	of,h
R4858	T8085	T8086	compound	TGF,β2
R4859	T8086	T8088	compound	β2,treatment
R486	T1297	T1296	pobj	alterations,by
R4860	T8087	T8086	punct	-,β2
R4861	T8088	T8084	pobj	treatment,of
R4862	T8089	T8081	punct	", ",increased
R4863	T8090	T8091	compound	Snail,promoter
R4864	T8091	T8092	compound	promoter,activity
R4865	T8092	T8081	nsubj	activity,increased
R4866	T8093	T8081	aux	had,increased
R4867	T8094	T8081	advmod	3-fold,increased
R4868	T8095	T8081	punct	", ",increased
R4869	T8096	T8081	cc	and,increased
R487	T1298	T1297	prep	in,alterations
R4870	T8097	T8098	prep	by,peaked
R4871	T8098	T8081	conj	peaked,increased
R4872	T8099	T8100	nummod	2,h
R4873	T8100	T8097	pobj	h,by
R4874	T8101	T8098	punct	", ",peaked
R4875	T8102	T8098	nsubj	it,peaked
R4876	T8103	T8098	prep	to,peaked
R4877	T8104	T8105	advmod	approximately,10-fold
R4878	T8105	T8103	pcomp	10-fold,to
R4879	T8106	T8105	prep	over,10-fold
R488	T1299	T1300	det	the,proliferation
R4880	T8107	T8108	compound	control,levels
R4881	T8108	T8106	pobj	levels,over
R4882	T8109	T8110	punct	(,5D
R4883	T8110	T8098	parataxis	5D,peaked
R4884	T8111	T8110	compound	Figure,5D
R4885	T8112	T8110	punct	),5D
R4886	T8113	T8098	punct	.,peaked
R4887	T8115	T8116	advmod	Thereafter,returned
R4888	T8117	T8116	punct	", ",returned
R4889	T8118	T8119	compound	Snail,promoter
R489	T1300	T1298	pobj	proliferation,in
R4890	T8119	T8120	compound	promoter,activity
R4891	T8120	T8116	nsubj	activity,returned
R4892	T8121	T8116	advmod	rapidly,returned
R4893	T8122	T8116	prep	to,returned
R4894	T8123	T8124	det	the,levels
R4895	T8124	T8122	pobj	levels,to
R4896	T8125	T8124	amod	basal,levels
R4897	T8126	T8124	acl	seen,levels
R4898	T8127	T8126	prep	in,seen
R4899	T8128	T8129	amod	unstimulated,keratinocytes
R490	T1301	T1300	punct	", ",proliferation
R4900	T8129	T8127	pobj	keratinocytes,in
R4901	T8130	T8116	punct	.,returned
R4902	T8132	T8133	det	The,kinetics
R4903	T8133	T8134	nsubj	kinetics,paralleled
R4904	T8135	T8133	prep	of,kinetics
R4905	T8136	T8137	compound	Snail,promoter
R4906	T8137	T8138	compound	promoter,activity
R4907	T8138	T8135	pobj	activity,of
R4908	T8139	T8134	advmod	closely,paralleled
R4909	T8140	T8134	dobj	those,paralleled
R491	T1302	T1300	conj	polarity,proliferation
R4910	T8141	T8140	acl	observed,those
R4911	T8142	T8141	prep	for,observed
R4912	T8143	T8144	compound	Snail,protein
R4913	T8144	T8145	compound	protein,induction
R4914	T8145	T8142	pobj	induction,for
R4915	T8146	T8134	punct	.,paralleled
R4916	T8148	T8149	advmod	Moreover,appeared
R4917	T8150	T8149	punct	", ",appeared
R4918	T8151	T8152	det	the,effects
R4919	T8152	T8149	nsubj	effects,appeared
R492	T1303	T1302	punct	", ",polarity
R4920	T8153	T8152	amod	stimulatory,effects
R4921	T8154	T8155	aux	to,be
R4922	T8155	T8149	xcomp	be,appeared
R4923	T8156	T8155	acomp	specific,be
R4924	T8157	T8156	prep	to,specific
R4925	T8158	T8159	compound	TGF,β2
R4926	T8159	T8157	pobj	β2,to
R4927	T8160	T8159	punct	-,β2
R4928	T8161	T8155	punct	", ",be
R4929	T8162	T8163	mark	since,abrogated
R493	T1304	T1302	conj	shape,polarity
R4930	T8163	T8155	advcl	abrogated,be
R4931	T8164	T8163	nsubjpass	they,abrogated
R4932	T8165	T8163	auxpass	were,abrogated
R4933	T8166	T8167	preconj	either,by
R4934	T8167	T8163	prep	by,abrogated
R4935	T8168	T8169	compound	heat,inactivation
R4936	T8169	T8167	pobj	inactivation,by
R4937	T8170	T8169	prep	of,inactivation
R4938	T8171	T8172	det	the,protein
R4939	T8172	T8170	pobj	protein,of
R494	T1305	T1304	punct	", ",shape
R4940	T8173	T8174	compound	TGF,β2
R4941	T8174	T8172	compound	β2,protein
R4942	T8175	T8174	punct	-,β2
R4943	T8176	T8167	cc	or,by
R4944	T8177	T8167	conj	by,by
R4945	T8178	T8177	pobj	mutation,by
R4946	T8179	T8178	prep	of,mutation
R4947	T8180	T8181	det	a,element
R4948	T8181	T8179	pobj	element,of
R4949	T8182	T8181	amod	putative,element
R495	T1306	T1304	cc	and,shape
R4950	T8183	T8184	compound	SMAD,binding
R4951	T8184	T8181	compound	binding,element
R4952	T8185	T8181	acl	located,element
R4953	T8186	T8187	quantmod	about,1.8
R4954	T8187	T8188	nummod	1.8,kb
R4955	T8188	T8185	npadvmod	kb,located
R4956	T8189	T8188	nummod	5,kb
R4957	T8190	T8188	punct	′,kb
R4958	T8191	T8188	prep	from,kb
R4959	T8192	T8193	det	the,site
R496	T1307	T1304	conj	adhesiveness,shape
R4960	T8193	T8191	pobj	site,from
R4961	T8194	T8193	compound	Snail,site
R4962	T8195	T8193	compound	transcription,site
R4963	T8196	T8193	compound	start,site
R4964	T8197	T8198	punct	(,5D
R4965	T8198	T8163	parataxis	5D,abrogated
R4966	T8199	T8198	compound	Figure,5D
R4967	T8200	T8198	punct	),5D
R4968	T8201	T8149	punct	.,appeared
R4969	T8203	T8204	advcl	Taken,suggested
R497	T1308	T1300	prep	of,proliferation
R4970	T8205	T8203	advmod	together,Taken
R4971	T8206	T8204	punct	", ",suggested
R4972	T8207	T8208	det	these,results
R4973	T8208	T8204	nsubj	results,suggested
R4974	T8209	T8210	mark	that,results
R4975	T8210	T8204	advcl	results,suggested
R4976	T8211	T8210	prep	in,results
R4977	T8212	T8211	pobj	keratinocytes,in
R4978	T8213	T8210	punct	", ",results
R4979	T8214	T8215	compound	TGF,β2
R498	T1309	T1310	amod	selected,cells
R4980	T8215	T8217	compound	β2,signaling
R4981	T8216	T8215	punct	-,β2
R4982	T8217	T8210	nsubj	signaling,results
R4983	T8218	T8210	prep	in,results
R4984	T8219	T8220	det	a,activation
R4985	T8220	T8218	pobj	activation,in
R4986	T8221	T8222	npadvmod	pSMAD2,dependent
R4987	T8222	T8220	amod	dependent,activation
R4988	T8223	T8222	punct	-,dependent
R4989	T8224	T8220	amod	transient,activation
R499	T1310	T1308	pobj	cells,of
R4990	T8225	T8220	prep	of,activation
R4991	T8226	T8227	det	the,gene
R4992	T8227	T8225	pobj	gene,of
R4993	T8228	T8227	compound	Snail,gene
R4994	T8229	T8210	punct	", ",results
R4995	T8230	T8210	cc	and,results
R4996	T8231	T8232	mark	that,relies
R4997	T8232	T8210	conj	relies,results
R4998	T8233	T8232	nsubj	maintenance,relies
R4999	T8234	T8233	prep	of,maintenance
R500	T1311	T1296	punct	", ",by
R5000	T8235	T8236	compound	Snail,protein
R5001	T8236	T8234	pobj	protein,of
R5002	T8237	T8232	punct	", ",relies
R5003	T8238	T8232	prep	in,relies
R5004	T8239	T8238	pobj	part,in
R5005	T8240	T8232	punct	", ",relies
R5006	T8241	T8232	prep	upon,relies
R5007	T8242	T8243	amod	sustained,activity
R5008	T8243	T8241	pobj	activity,upon
R5009	T8244	T8243	compound	promoter,activity
R501	T1312	T1313	advmod	as,as
R5010	T8245	T8204	punct	.,suggested
R5011	T8247	T8248	det	The,brevity
R5012	T8248	T8249	nsubj	brevity,resembled
R5013	T8250	T8248	prep	of,brevity
R5014	T8251	T8252	nmod	Snail,gene
R5015	T8252	T8253	nmod	gene,induction
R5016	T8253	T8250	pobj	induction,of
R5017	T8254	T8252	cc	and,gene
R5018	T8255	T8252	conj	protein,gene
R5019	T8256	T8253	prep	in,induction
R502	T1313	T1296	cc	as,by
R5020	T8257	T8258	compound	TGF,β2
R5021	T8258	T8260	npadvmod	β2,treated
R5022	T8259	T8258	punct	-,β2
R5023	T8260	T8261	amod	treated,keratinocytes
R5024	T8261	T8256	pobj	keratinocytes,in
R5025	T8262	T8261	amod	cultured,keratinocytes
R5026	T8263	T8264	det	the,appearance
R5027	T8264	T8249	dobj	appearance,resembled
R5028	T8265	T8264	amod	temporal,appearance
R5029	T8266	T8264	prep	of,appearance
R503	T1314	T1313	advmod	well,as
R5030	T8267	T8268	compound	Snail,mRNA
R5031	T8268	T8266	pobj	mRNA,of
R5032	T8269	T8268	cc	and,mRNA
R5033	T8270	T8268	conj	protein,mRNA
R5034	T8271	T8264	prep	at,appearance
R5035	T8272	T8273	det	the,initiation
R5036	T8273	T8271	pobj	initiation,at
R5037	T8274	T8273	prep	of,initiation
R5038	T8275	T8276	compound	hair,follicle
R5039	T8276	T8277	compound	follicle,morphogenesis
R504	T1315	T1296	conj	by,by
R5040	T8277	T8274	pobj	morphogenesis,of
R5041	T8278	T8249	prep	in,resembled
R5042	T8279	T8280	amod	embryonic,skin
R5043	T8280	T8278	pobj	skin,in
R5044	T8281	T8280	compound	mouse,skin
R5045	T8282	T8249	punct	.,resembled
R5046	T8284	T8285	aux	To,test
R5047	T8285	T8286	advcl	test,analyzed
R5048	T8287	T8288	mark	whether,required
R5049	T8288	T8285	ccomp	required,test
R505	T1316	T1315	pobj	modifications,by
R5050	T8289	T8290	compound	TGF,β2
R5051	T8290	T8288	nsubjpass	β2,required
R5052	T8291	T8290	punct	-,β2
R5053	T8292	T8288	aux	might,required
R5054	T8293	T8288	auxpass	be,required
R5055	T8294	T8288	prep	for,required
R5056	T8295	T8296	compound	Snail,induction
R5057	T8296	T8294	pobj	induction,for
R5058	T8297	T8296	prep	in,induction
R5059	T8298	T8299	compound	hair,bud
R506	T1317	T1316	prep	in,modifications
R5060	T8299	T8300	compound	bud,formation
R5061	T8300	T8297	pobj	formation,in
R5062	T8301	T8302	advmod	in,vivo
R5063	T8302	T8288	advmod	vivo,required
R5064	T8303	T8286	punct	", ",analyzed
R5065	T8304	T8286	nsubj	we,analyzed
R5066	T8305	T8286	advmod	first,analyzed
R5067	T8306	T8307	mark	whether,expressed
R5068	T8307	T8286	ccomp	expressed,analyzed
R5069	T8308	T8309	compound	TGF,β2
R507	T1318	T1319	poss	their,lamina
R5070	T8309	T8307	nsubjpass	β2,expressed
R5071	T8310	T8309	punct	-,β2
R5072	T8311	T8307	auxpass	was,expressed
R5073	T8312	T8307	prep	in,expressed
R5074	T8313	T8312	cc	or,in
R5075	T8314	T8312	conj	around,in
R5076	T8315	T8316	det	the,bud
R5077	T8316	T8314	pobj	bud,around
R5078	T8317	T8316	compound	hair,bud
R5079	T8318	T8286	punct	.,analyzed
R508	T1319	T1317	pobj	lamina,in
R5080	T8320	T8321	advcl	Consistent,labeled
R5081	T8322	T8320	prep	with,Consistent
R5082	T8323	T8324	amod	previous,observations
R5083	T8324	T8322	pobj	observations,with
R5084	T8325	T8326	punct	[,33
R5085	T8326	T8320	parataxis	33,Consistent
R5086	T8327	T8326	punct	],33
R5087	T8328	T8321	punct	", ",labeled
R5088	T8329	T8330	det	an,antibody
R5089	T8330	T8321	nsubj	antibody,labeled
R509	T1320	T1319	amod	underlying,lamina
R5090	T8331	T8332	amod	anti-TGF,β2
R5091	T8332	T8330	compound	β2,antibody
R5092	T8333	T8332	punct	-,β2
R5093	T8334	T8335	amod	developing,buds
R5094	T8335	T8321	dobj	buds,labeled
R5095	T8336	T8335	compound	hair,buds
R5096	T8337	T8338	punct	(,6A
R5097	T8338	T8321	parataxis	6A,labeled
R5098	T8339	T8338	compound	Figure,6A
R5099	T8340	T8338	punct	),6A
R510	T1321	T1319	amod	basal,lamina
R5100	T8341	T8321	punct	.,labeled
R5101	T8343	T8344	det	This,labeling
R5102	T8344	T8345	nsubj	labeling,appeared
R5103	T8346	T8347	aux	to,be
R5104	T8347	T8345	xcomp	be,appeared
R5105	T8348	T8347	acomp	specific,be
R5106	T8349	T8350	mark	as,judged
R5107	T8350	T8347	advcl	judged,be
R5108	T8351	T8350	prep	by,judged
R5109	T8352	T8353	det	the,lack
R511	T1322	T1293	punct	.,accompanied
R5110	T8353	T8351	pobj	lack,by
R5111	T8354	T8353	prep	of,lack
R5112	T8355	T8354	pobj	staining,of
R5113	T8356	T8353	prep	in,lack
R5114	T8357	T8358	compound	follicle,buds
R5115	T8358	T8356	pobj	buds,in
R5116	T8359	T8358	prep	from,buds
R5117	T8360	T8359	pobj	mice,from
R5118	T8361	T8360	amod	homozygous,mice
R5119	T8362	T8361	prep	for,homozygous
R512	T1324	T1325	advmod	Thus,orchestrated
R5120	T8363	T8364	det	a,mutation
R5121	T8364	T8362	pobj	mutation,for
R5122	T8365	T8366	nmod	TGF,β2
R5123	T8366	T8364	nmod	β2,mutation
R5124	T8367	T8366	punct	-,β2
R5125	T8368	T8364	amod	null,mutation
R5126	T8369	T8370	punct	(,34
R5127	T8370	T8345	parataxis	34,appeared
R5128	T8371	T8372	compound	Figure,6A
R5129	T8372	T8370	dep	6A,34
R513	T1326	T1325	punct	", ",orchestrated
R5130	T8373	T8370	punct	;,34
R5131	T8374	T8370	punct	[,34
R5132	T8375	T8370	punct	],34
R5133	T8376	T8370	punct	),34
R5134	T8377	T8345	punct	.,appeared
R5135	T8379	T8380	advmod	Moreover,expressed
R5136	T8381	T8380	punct	", ",expressed
R5137	T8382	T8383	det	the,effector
R5138	T8383	T8380	nsubjpass	effector,expressed
R5139	T8384	T8383	amod	downstream,effector
R514	T1327	T1328	amod	extracellular,crosstalk
R5140	T8385	T8383	prep	of,effector
R5141	T8386	T8387	compound	TGF,β2
R5142	T8387	T8389	compound	β2,signaling
R5143	T8388	T8387	punct	-,β2
R5144	T8389	T8385	pobj	signaling,of
R5145	T8390	T8383	punct	", ",effector
R5146	T8391	T8383	appos	pSMAD2,effector
R5147	T8392	T8380	punct	", ",expressed
R5148	T8393	T8380	auxpass	was,expressed
R5149	T8394	T8380	advmod	also,expressed
R515	T1328	T1325	nsubjpass	crosstalk,orchestrated
R5150	T8395	T8380	prep	in,expressed
R5151	T8396	T8397	nmod	WT,buds
R5152	T8397	T8395	pobj	buds,in
R5153	T8398	T8396	punct	", ",WT
R5154	T8399	T8396	cc	but,WT
R5155	T8400	T8399	neg	not,but
R5156	T8401	T8402	compound	TGF,β2
R5157	T8402	T8404	npadvmod	β2,null
R5158	T8403	T8402	punct	-,β2
R5159	T8404	T8396	conj	null,WT
R516	T1329	T1330	amod	epithelial,mesenchymal
R5160	T8405	T8404	punct	-,null
R5161	T8406	T8397	punct	", ",buds
R5162	T8407	T8397	compound	hair,buds
R5163	T8408	T8409	punct	(,6B
R5164	T8409	T8380	parataxis	6B,expressed
R5165	T8410	T8409	compound	Figure,6B
R5166	T8411	T8409	punct	),6B
R5167	T8412	T8380	punct	.,expressed
R5168	T8414	T8415	advmod	Together,underscore
R5169	T8416	T8415	punct	", ",underscore
R517	T1330	T1328	amod	mesenchymal,crosstalk
R5170	T8417	T8418	det	these,data
R5171	T8418	T8415	nsubj	data,underscore
R5172	T8419	T8420	det	the,importance
R5173	T8420	T8415	dobj	importance,underscore
R5174	T8421	T8420	prep	of,importance
R5175	T8422	T8423	det	the,isoform
R5176	T8423	T8421	pobj	isoform,of
R5177	T8424	T8425	compound	TGF,β2
R5178	T8425	T8423	compound	β2,isoform
R5179	T8426	T8425	punct	-,β2
R518	T1331	T1330	punct	-,mesenchymal
R5180	T8427	T8415	prep	despite,underscore
R5181	T8428	T8427	pobj	expression,despite
R5182	T8429	T8428	prep	of,expression
R5183	T8430	T8431	preconj	both,β1
R5184	T8431	T8429	pobj	β1,of
R5185	T8432	T8431	compound	TGF,β1
R5186	T8433	T8431	punct	-,β1
R5187	T8434	T8431	cc	and,β1
R5188	T8435	T8436	compound	TGF,β2
R5189	T8436	T8431	conj	β2,β1
R519	T1332	T1325	aux	must,orchestrated
R5190	T8437	T8436	punct	-,β2
R5191	T8438	T8428	prep	in,expression
R5192	T8439	T8440	amod	developing,buds
R5193	T8440	T8438	pobj	buds,in
R5194	T8441	T8440	compound	hair,buds
R5195	T8442	T8428	prep	at,expression
R5196	T8443	T8444	det	this,stage
R5197	T8444	T8442	pobj	stage,at
R5198	T8445	T8415	punct	.,underscore
R5199	T8447	T8448	aux	To,explore
R520	T1333	T1325	auxpass	be,orchestrated
R5200	T8448	T8450	advcl	explore,examined
R5201	T8449	T8448	advmod	further,explore
R5202	T8451	T8452	det	the,relation
R5203	T8452	T8448	dobj	relation,explore
R5204	T8453	T8452	amod	possible,relation
R5205	T8454	T8452	prep	between,relation
R5206	T8455	T8454	pobj	Snail,between
R5207	T8456	T8455	cc	and,Snail
R5208	T8457	T8458	compound	TGF,β2
R5209	T8458	T8455	conj	β2,Snail
R521	T1334	T1325	advmod	intricately,orchestrated
R5210	T8459	T8458	punct	-,β2
R5211	T8460	T8450	punct	", ",examined
R5212	T8461	T8450	nsubj	we,examined
R5213	T8462	T8463	det	the,status
R5214	T8463	T8450	dobj	status,examined
R5215	T8464	T8463	prep	of,status
R5216	T8465	T8466	compound	Snail,expression
R5217	T8466	T8464	pobj	expression,of
R5218	T8467	T8463	prep	in,status
R5219	T8468	T8469	compound	TGF,β2
R522	T1335	T1336	aux	to,couple
R5220	T8469	T8471	npadvmod	β2,null
R5221	T8470	T8469	punct	-,β2
R5222	T8471	T8473	amod	null,buds
R5223	T8472	T8471	punct	-,null
R5224	T8473	T8467	pobj	buds,in
R5225	T8474	T8473	compound	hair,buds
R5226	T8475	T8450	punct	.,examined
R5227	T8477	T8478	mark	As,judged
R5228	T8478	T8479	advcl	judged,was
R5229	T8480	T8478	prep	by,judged
R523	T1336	T1325	advcl	couple,orchestrated
R5230	T8481	T8480	pobj	immunohistochemistry,by
R5231	T8482	T8479	punct	", ",was
R5232	T8483	T8484	compound	Snail,protein
R5233	T8484	T8479	nsubj	protein,was
R5234	T8485	T8479	acomp	absent,was
R5235	T8486	T8485	prep	from,absent
R5236	T8487	T8488	compound	E17.5,skin
R5237	T8488	T8486	pobj	skin,from
R5238	T8489	T8488	prep	of,skin
R5239	T8490	T8491	compound	TGF,β2
R524	T1337	T1338	det	the,determination
R5240	T8491	T8493	npadvmod	β2,null
R5241	T8492	T8491	punct	-,β2
R5242	T8493	T8495	amod	null,embryos
R5243	T8494	T8493	punct	-,null
R5244	T8495	T8489	pobj	embryos,of
R5245	T8496	T8486	cc	but,from
R5246	T8497	T8496	neg	not,but
R5247	T8498	T8486	conj	from,from
R5248	T8499	T8498	pobj	that,from
R5249	T8500	T8499	prep	of,that
R525	T1338	T1336	dobj	determination,couple
R5250	T8501	T8502	compound	control,littermates
R5251	T8502	T8500	pobj	littermates,of
R5252	T8503	T8504	punct	(,6C
R5253	T8504	T8479	parataxis	6C,was
R5254	T8505	T8504	compound	Figure,6C
R5255	T8506	T8504	punct	),6C
R5256	T8507	T8479	punct	.,was
R5257	T8509	T8510	det	This,effect
R5258	T8510	T8511	nsubj	effect,appeared
R5259	T8512	T8513	aux	to,exerted
R526	T1339	T1338	prep	of,determination
R5260	T8513	T8511	xcomp	exerted,appeared
R5261	T8514	T8513	auxpass	be,exerted
R5262	T8515	T8513	prep	at,exerted
R5263	T8516	T8517	det	the,level
R5264	T8517	T8515	pobj	level,at
R5265	T8518	T8517	amod	transcriptional,level
R5266	T8519	T8511	punct	", ",appeared
R5267	T8520	T8521	mark	since,found
R5268	T8521	T8511	advcl	found,appeared
R5269	T8522	T8523	compound	Snail,mRNAs
R527	T1340	T1341	amod	distinct,fates
R5270	T8523	T8521	nsubjpass	mRNAs,found
R5271	T8524	T8521	auxpass	were,found
R5272	T8525	T8521	advmod	also,found
R5273	T8526	T8521	neg	not,found
R5274	T8527	T8521	prep	in,found
R5275	T8528	T8529	compound	TGF,β2
R5276	T8529	T8531	npadvmod	β2,null
R5277	T8530	T8529	punct	-,β2
R5278	T8531	T8532	amod	null,buds
R5279	T8532	T8527	pobj	buds,in
R528	T1341	T1339	pobj	fates,of
R5280	T8533	T8532	compound	hair,buds
R5281	T8534	T8521	prep	under,found
R5282	T8535	T8534	pobj	conditions,under
R5283	T8536	T8537	prep	in,detected
R5284	T8537	T8535	relcl	detected,conditions
R5285	T8538	T8536	pobj	which,in
R5286	T8539	T8540	det	the,signal
R5287	T8559	T8557	prep	in,detected
R5288	T8540	T8537	nsubjpass	signal,detected
R5289	T8541	T8537	auxpass	was,detected
R529	T1342	T1341	compound	cell,fates
R5290	T8560	T8561	nummod	2,wk
R5291	T8561	T8563	npadvmod	wk,old
R5292	T8542	T8537	advmod	readily,detected
R5293	T8562	T8561	punct	-,wk
R5294	T8563	T8565	amod	old,mice
R5295	T8564	T8563	punct	-,old
R5296	T8543	T8537	prep	in,detected
R5297	T8565	T8559	pobj	mice,in
R5298	T8566	T8567	compound	K14,Smad2
R5299	T8567	T8565	compound	Smad2,mice
R530	T1343	T1336	prep	with,couple
R5300	T8568	T8567	punct	-,Smad2
R5301	T8569	T8565	compound	Tg,mice
R5302	T8544	T8545	det	the,buds
R5303	T8570	T8565	punct	", ",mice
R5304	T8571	T8572	dep	which,display
R5305	T8572	T8565	relcl	display,mice
R5306	T8545	T8543	pobj	buds,in
R5307	T8573	T8574	amod	elevated,signaling
R5308	T8546	T8545	compound	hair,buds
R5309	T8574	T8572	dobj	signaling,display
R531	T1344	T1345	det	the,remodeling
R5310	T8575	T8576	compound	TGF,β
R5311	T8547	T8545	prep	of,buds
R5312	T8576	T8574	compound	β,signaling
R5313	T8577	T8576	punct	-,β
R5314	T8578	T8572	prep	in,display
R5315	T8548	T8549	compound	littermate,skin
R5316	T8549	T8547	pobj	skin,of
R5317	T8579	T8578	pobj	skin,in
R5318	T8580	T8581	punct	[,35
R5319	T8550	T8551	punct	(,6D
R532	T1345	T1343	pobj	remodeling,with
R5320	T8581	T8559	parataxis	35,in
R5321	T8582	T8581	punct	],35
R5322	T8583	T8557	punct	", ",detected
R5323	T8551	T8521	parataxis	6D,found
R5324	T8584	T8585	compound	Snail,protein
R5325	T8585	T8557	nsubjpass	protein,detected
R5326	T8552	T8551	compound	Figure,6D
R5327	T8586	T8557	auxpass	was,detected
R5328	T8587	T8557	advmod	readily,detected
R5329	T8553	T8551	punct	),6D
R533	T1346	T1345	amod	contemporaneous,remodeling
R5330	T8588	T8557	prep	by,detected
R5331	T8554	T8511	punct	.,appeared
R5332	T8589	T8590	compound	Western,blot
R5333	T8590	T8591	compound	blot,analyses
R5334	T8591	T8588	pobj	analyses,by
R5335	T8592	T8557	punct	", ",detected
R5336	T8593	T8594	advmod	where,found
R5337	T8556	T8557	advmod	Conversely,detected
R5338	T8594	T8557	advcl	found,detected
R5339	T8595	T8594	nsubjpass	it,found
R534	T1347	T1345	prep	of,remodeling
R5340	T8596	T8594	auxpass	was,found
R5341	T8597	T8594	neg	not,found
R5342	T8558	T8557	punct	", ",detected
R5343	T8598	T8594	prep	in,found
R5344	T8599	T8600	amod	postnatal,skin
R5345	T8600	T8598	pobj	skin,in
R5346	T8601	T8602	punct	(,6E
R5347	T8664	T8660	advmod	still,formed
R5348	T8602	T8557	parataxis	6E,detected
R5349	T8603	T8602	compound	Figure,6E
R535	T1348	T1349	det	the,properties
R5350	T8604	T8602	punct	),6E
R5351	T8605	T8557	punct	.,detected
R5352	T8607	T8608	advcl	Taken,provide
R5353	T8609	T8607	advmod	together,Taken
R5354	T8610	T8608	punct	", ",provide
R5355	T8611	T8612	det	these,results
R5356	T8666	T8660	prep	in,formed
R5357	T8612	T8608	nsubj	results,provide
R5358	T8613	T8614	amod	compelling,evidence
R5359	T8614	T8608	dobj	evidence,provide
R536	T1349	T1347	pobj	properties,of
R5360	T8615	T8616	mark	that,is
R5361	T8616	T8614	acl	is,evidence
R5362	T8667	T8668	compound	TGF,β2
R5363	T8617	T8618	compound	TGF,β2
R5364	T8618	T8616	nsubj	β2,is
R5365	T8619	T8618	punct	-,β2
R5366	T8668	T8670	npadvmod	β2,null
R5367	T8620	T8621	advmod	functionally,important
R5368	T8621	T8616	acomp	important,is
R5369	T8669	T8668	punct	-,β2
R537	T1350	T1349	amod	physical,properties
R5370	T8622	T8621	prep	for,important
R5371	T8623	T8622	pcomp	inducing,for
R5372	T8624	T8625	compound	Snail,expression
R5373	T8670	T8671	amod	null,skin
R5374	T8625	T8623	dobj	expression,inducing
R5375	T8626	T8625	compound	gene,expression
R5376	T8627	T8623	prep	in,inducing
R5377	T8671	T8666	pobj	skin,in
R5378	T8628	T8629	det	a,manner
R5379	T8629	T8627	pobj	manner,in
R538	T1351	T1350	cc	and,physical
R5380	T8672	T8665	punct	", ",reduced
R5381	T8630	T8631	npadvmod	pSMAD,dependent
R5382	T8631	T8629	amod	dependent,manner
R5383	T8632	T8631	punct	-,dependent
R5384	T8673	T8674	poss	their,number
R5385	T8633	T8623	prep	in,inducing
R5386	T8634	T8635	amod	developing,buds
R5387	T8635	T8633	pobj	buds,in
R5388	T8674	T8665	nsubjpass	number,reduced
R5389	T8636	T8635	compound	hair,buds
R539	T1352	T1350	conj	structural,physical
R5390	T8637	T8608	punct	.,provide
R5391	T8675	T8665	auxpass	was,reduced
R5392	T8639	T8640	mark	Whether,contributes
R5393	T8640	T8644	csubjpass	contributes,addressed
R5394	T8676	T8665	prep	by,reduced
R5395	T8641	T8642	compound	pMARK,activity
R5396	T8642	T8640	nsubj	activity,contributes
R5397	T8643	T8640	advmod	also,contributes
R5398	T8677	T8678	advmod	approximately,50
R5399	T8645	T8640	prep	to,contributes
R540	T1353	T1349	prep	of,properties
R5400	T8646	T8647	compound	Snail,induction
R5401	T8647	T8645	pobj	induction,to
R5402	T8648	T8644	auxpass	was,addressed
R5403	T8649	T8644	neg	not,addressed
R5404	T8678	T8679	nummod	50,%
R5405	T8650	T8644	prep	in,addressed
R5406	T8651	T8652	det	the,study
R5407	T8652	T8650	pobj	study,in
R5408	T8679	T8676	pobj	%,by
R5409	T8653	T8652	amod	present,study
R541	T1354	T1355	det	the,cell
R5410	T8654	T8655	punct	[,15
R5411	T8655	T8644	parataxis	15,addressed
R5412	T8680	T8681	punct	[,32
R5413	T8656	T8655	punct	],15
R5414	T8657	T8644	punct	.,addressed
R5415	T8681	T8665	parataxis	32,reduced
R5416	T8659	T8660	mark	Although,formed
R5417	T8660	T8665	advcl	formed,reduced
R5418	T8661	T8662	det	some,buds
R5419	T8682	T8681	punct	],32
R542	T1355	T1353	pobj	cell,of
R5420	T8662	T8660	nsubj	buds,formed
R5421	T8663	T8662	compound	hair,buds
R5422	T8683	T8665	punct	.,reduced
R5423	T8685	T8686	advmod	Thus,appear
R5424	T8687	T8686	punct	", ",appear
R5425	T8770	T8772	compound	β2,signaling
R5426	T8688	T8689	mark	although,impacts
R5427	T8689	T8686	advcl	impacts,appear
R5428	T8771	T8770	punct	-,β2
R5429	T8772	T8766	nsubj	signaling,appeared
R543	T1356	T1325	punct	.,orchestrated
R5430	T8690	T8691	det	the,pathway
R5431	T8773	T8774	aux	to,be
R5432	T8774	T8766	xcomp	be,appeared
R5433	T8775	T8776	det	an,factor
R5434	T8691	T8689	nsubj	pathway,impacts
R5435	T8776	T8774	attr	factor,be
R5436	T8777	T8776	amod	important,factor
R5437	T8692	T8691	acl	mediated,pathway
R5438	T8778	T8776	prep	for,factor
R5439	T8779	T8780	det	the,proliferation
R544	T1358	T1359	prep	Among,offer
R5440	T8693	T8692	prep	by,mediated
R5441	T8780	T8778	pobj	proliferation,for
R5442	T8781	T8780	amod	early,proliferation
R5443	T8782	T8783	dep	that,occurs
R5444	T8694	T8695	compound	TGF,β2
R5445	T8783	T8780	relcl	occurs,proliferation
R5446	T8784	T8783	prep	in,occurs
R5447	T8695	T8697	compound	β2,signaling
R5448	T8785	T8786	det	the,buds
R5449	T8786	T8784	pobj	buds,in
R545	T1360	T1361	det	the,organs
R5450	T8787	T8786	amod	developing,buds
R5451	T8696	T8695	punct	-,β2
R5452	T8788	T8786	compound	hair,buds
R5453	T8789	T8774	punct	", ",be
R5454	T8790	T8791	mark	as,judged
R5455	T8791	T8774	advcl	judged,be
R5456	T8792	T8791	prep	by,judged
R5457	T8793	T8794	amod	anti-Ki67,immunofluorescence
R5458	T8697	T8693	pobj	signaling,by
R5459	T8794	T8792	pobj	immunofluorescence,by
R546	T1361	T1358	pobj	organs,Among
R5460	T8795	T8793	cc	and,anti-Ki67
R5461	T8698	T8699	det	the,step
R5462	T8796	T8793	conj	anti-pMAPK,anti-Ki67
R5463	T8797	T8798	punct	(,6F
R5464	T8798	T8774	parataxis	6F,be
R5465	T8699	T8689	dobj	step,impacts
R5466	T8799	T8798	compound	Figure,6F
R5467	T8800	T8798	cc	and,6F
R5468	T8801	T8798	conj	6G,6F
R5469	T8700	T8699	amod	earliest,step
R547	T1362	T1361	amod	few,organs
R5470	T8802	T8798	punct	),6F
R5471	T8803	T8766	punct	.,appeared
R5472	T8701	T8699	prep	of,step
R5473	T8805	T8806	mark	If,stimulates
R5474	T8806	T8810	advcl	stimulates,expected
R5475	T8702	T8703	amod	epithelial,invagination
R5476	T8807	T8808	compound	TGF,β2
R5477	T8808	T8806	nsubj	β2,stimulates
R5478	T8809	T8808	punct	-,β2
R5479	T8703	T8701	pobj	invagination,of
R548	T1363	T1361	amod	dispensable,organs
R5480	T8811	T8812	compound	Snail,expression
R5481	T8812	T8806	dobj	expression,stimulates
R5482	T8704	T8686	punct	", ",appear
R5483	T8813	T8806	prep	in,stimulates
R5484	T8814	T8815	amod	developing,buds
R5485	T8815	T8813	pobj	buds,in
R5486	T8705	T8686	nsubj	it,appear
R5487	T8816	T8810	punct	", ",expected
R5488	T8817	T8810	nsubjpass	loss,expected
R5489	T8818	T8817	prep	of,loss
R549	T1364	T1359	punct	", ",offer
R5490	T8706	T8686	aux	does,appear
R5491	T8819	T8820	det	this,morphogen
R5492	T8820	T8818	pobj	morphogen,of
R5493	T8707	T8686	neg	not,appear
R5494	T8821	T8810	aux	would,expected
R5495	T8822	T8810	auxpass	be,expected
R5496	T8823	T8824	aux	to,affect
R5497	T8708	T8709	aux	to,be
R5498	T8824	T8810	xcomp	affect,expected
R5499	T8825	T8826	det	the,expression
R550	T1365	T1366	compound	hair,follicles
R5500	T8826	T8824	dobj	expression,affect
R5501	T8709	T8686	xcomp	be,appear
R5502	T8827	T8826	prep	of,expression
R5503	T8828	T8827	pobj	genes,of
R5504	T8829	T8830	dep	that,repressed
R5505	T8830	T8828	relcl	repressed,genes
R5506	T8831	T8830	auxpass	are,repressed
R5507	T8832	T8830	advmod	typically,repressed
R5508	T8710	T8709	acomp	essential,be
R5509	T8833	T8830	agent	by,repressed
R551	T1366	T1359	nsubj	follicles,offer
R5510	T8834	T8833	pobj	Snail,by
R5511	T8835	T8810	punct	.,expected
R5512	T8711	T8710	prep	for,essential
R5513	T8712	T8713	compound	bud,morphogenesis
R5514	T8837	T8838	mark	Since,is
R5515	T8713	T8711	pobj	morphogenesis,for
R5516	T8838	T8847	advcl	is,investigated
R5517	T8839	T8840	det	a,target
R5518	T8714	T8686	punct	.,appear
R5519	T8840	T8838	nsubj	target,is
R552	T1367	T1368	det	an,system
R5520	T8841	T8840	amod	major,target
R5521	T8842	T8840	prep	for,target
R5522	T8843	T8844	npadvmod	Snail,mediated
R5523	T8716	T8717	advcl	Consistent,took
R5524	T8844	T8846	amod	mediated,repression
R5525	T8845	T8844	punct	-,mediated
R5526	T8846	T8842	pobj	repression,for
R5527	T8718	T8716	prep	with,Consistent
R5528	T8848	T8849	det	the,gene
R5529	T8849	T8838	attr	gene,is
R553	T1368	T1359	dobj	system,offer
R5530	T8850	T8851	compound	E,cadherin
R5531	T8719	T8720	det	this,notion
R5532	T8720	T8718	pobj	notion,with
R5533	T8721	T8717	punct	", ",took
R5534	T8851	T8849	compound	cadherin,gene
R5535	T8722	T8723	compound	basement,membrane
R5536	T8723	T8724	compound	membrane,remodeling
R5537	T8852	T8851	punct	-,cadherin
R5538	T8853	T8854	punct	[,13
R5539	T8854	T8838	parataxis	13,is
R554	T1369	T1368	amod	excellent,system
R5540	T8724	T8717	nsubj	remodeling,took
R5541	T8855	T8854	nummod	12,13
R5542	T8856	T8854	punct	",",13
R5543	T8725	T8717	advmod	still,took
R5544	T8857	T8854	punct	],13
R5545	T8858	T8847	punct	", ",investigated
R5546	T8859	T8847	nsubj	we,investigated
R5547	T8726	T8717	dobj	place,took
R5548	T8860	T8861	det	the,status
R5549	T8861	T8847	dobj	status,investigated
R555	T1370	T1368	compound	model,system
R5550	T8727	T8717	prep	in,took
R5551	T8862	T8861	prep	of,status
R5552	T8863	T8864	compound	E,cadherin
R5553	T8864	T8862	pobj	cadherin,of
R5554	T8728	T8729	det	the,buds
R5555	T8865	T8864	punct	-,cadherin
R5556	T8866	T8847	prep	in,investigated
R5557	T8867	T8868	compound	TGF,β2
R5558	T8868	T8870	npadvmod	β2,null
R5559	T8869	T8868	punct	-,β2
R556	T1371	T1372	aux	to,study
R5560	T8870	T8872	amod	null,buds
R5561	T8729	T8727	pobj	buds,in
R5562	T8871	T8870	punct	-,null
R5563	T8872	T8866	pobj	buds,in
R5564	T8873	T8847	punct	.,investigated
R5565	T8730	T8731	compound	TGF,β2
R5566	T8875	T8876	mark	As,shown
R5567	T8731	T8733	npadvmod	β2,null
R5568	T8732	T8731	punct	-,β2
R5569	T8733	T8729	amod	null,buds
R557	T1372	T1368	advcl	study,system
R5570	T8734	T8733	punct	-,null
R5571	T8876	T8877	advcl	shown,displayed
R5572	T8735	T8717	punct	", ",took
R5573	T8878	T8876	prep	in,shown
R5574	T8736	T8737	mark	as,judged
R5575	T8879	T8880	compound	Figure,6H
R5576	T8880	T8878	pobj	6H,in
R5577	T8881	T8877	punct	", ",displayed
R5578	T8737	T8717	advcl	judged,took
R5579	T8882	T8883	compound	hair,buds
R558	T1373	T1374	amod	epithelial,bud
R5580	T8883	T8877	nsubj	buds,displayed
R5581	T8884	T8883	prep	in,buds
R5582	T8738	T8737	prep	by,judged
R5583	T8885	T8886	compound	TGF,β2
R5584	T8886	T8888	npadvmod	β2,null
R5585	T8887	T8886	punct	-,β2
R5586	T8739	T8738	pobj	immunofluorescence,by
R5587	T8888	T8889	amod	null,skin
R5588	T8889	T8884	pobj	skin,in
R5589	T8740	T8739	prep	with,immunofluorescence
R559	T1374	T1375	compound	bud,formation
R5590	T8890	T8891	amod	elevated,staining
R5591	T8891	T8877	dobj	staining,displayed
R5592	T8892	T8891	compound	immunofluorescence,staining
R5593	T8741	T8740	pobj	antibodies,with
R5594	T8893	T8877	advcl	relative,displayed
R5595	T8894	T8893	prep	to,relative
R5596	T8895	T8896	poss	their,counterparts
R5597	T8742	T8741	prep	against,antibodies
R5598	T8896	T8894	pobj	counterparts,to
R5599	T8897	T8896	compound	WT,counterparts
R560	T1375	T1372	dobj	formation,study
R5600	T8898	T8877	punct	.,displayed
R5601	T8743	T8744	compound	β4,integrin
R5602	T8900	T8901	advmod	Previously,demonstrated
R5603	T8744	T8742	pobj	integrin,against
R5604	T8902	T8901	nsubj	we,demonstrated
R5605	T8903	T8904	mark	that,required
R5606	T8745	T8744	punct	", ",integrin
R5607	T8904	T8901	ccomp	required,demonstrated
R5608	T8905	T8906	det	the,action
R5609	T8906	T8904	nsubjpass	action,required
R561	T1376	T1359	punct	.,offer
R5610	T8907	T8906	amod	concerted,action
R5611	T8908	T8906	prep	of,action
R5612	T8746	T8747	det	an,component
R5613	T8909	T8910	det	the,signals
R5614	T8910	T8908	pobj	signals,of
R5615	T8911	T8910	amod	extracellular,signals
R5616	T8747	T8744	appos	component,integrin
R5617	T8912	T8910	appos	Wnt,signals
R5618	T8913	T8912	cc	and,Wnt
R5619	T8914	T8912	conj	noggin,Wnt
R562	T1378	T1379	amod	Mammalian,epithelium
R5620	T8748	T8747	amod	integral,component
R5621	T8915	T8904	auxpass	are,required
R5622	T8916	T8917	mark	for,repress
R5623	T8749	T8747	prep	of,component
R5624	T8750	T8751	npadvmod	keratinocyte,mediated
R5625	T8917	T8904	advcl	repress,required
R5626	T8918	T8919	det	the,generation
R5627	T8751	T8753	amod	mediated,adhesion
R5628	T8919	T8917	nsubj	generation,repress
R5629	T8920	T8919	prep	of,generation
R563	T1379	T1381	nsubj	epithelium,begins
R5630	T8921	T8922	det	a,complex
R5631	T8752	T8751	punct	-,mediated
R5632	T8922	T8920	pobj	complex,of
R5633	T8923	T8922	nmod	LEF,complex
R5634	T8924	T8923	punct	-,LEF
R5635	T8753	T8749	pobj	adhesion,of
R5636	T8925	T8923	nummod	1,LEF
R5637	T8926	T8923	punct	/,LEF
R5638	T8927	T8928	compound	β,catenin
R5639	T8754	T8753	prep	to,adhesion
R564	T1380	T1379	compound	skin,epithelium
R5640	T8928	T8923	appos	catenin,LEF
R5641	T8929	T8928	punct	-,catenin
R5642	T8755	T8756	poss	its,membrane
R5643	T8930	T8922	compound	transcription,complex
R5644	T8931	T8917	aux	to,repress
R5645	T8932	T8933	compound	E,cadherin
R5646	T8756	T8754	pobj	membrane,to
R5647	T8933	T8935	compound	cadherin,transcription
R5648	T8934	T8933	punct	-,cadherin
R5649	T8757	T8756	amod	underlying,membrane
R565	T1382	T1381	prep	as,begins
R5650	T8935	T8917	dobj	transcription,repress
R5651	T8936	T8917	prep	at,repress
R5652	T8937	T8938	det	the,onset
R5653	T8758	T8756	compound	basement,membrane
R5654	T8938	T8936	pobj	onset,at
R5655	T8939	T8938	prep	of,onset
R5656	T8759	T8760	punct	(,6F
R5657	T8940	T8941	compound	hair,fate
R5658	T8941	T8942	compound	fate,specification
R5659	T8942	T8939	pobj	specification,of
R566	T1383	T1384	det	a,sheet
R5660	T8943	T8901	punct	.,demonstrated
R5661	T8760	T8717	parataxis	6F,took
R5662	T8945	T8946	mark	As,shown
R5663	T8946	T8947	advcl	shown,exhibited
R5664	T8948	T8946	prep	in,shown
R5665	T8761	T8760	compound	Figure,6F
R5666	T8949	T8950	compound	Figure,6I
R5667	T8950	T8948	pobj	6I,in
R5668	T8762	T8760	punct	),6F
R5669	T8951	T8950	cc	and,6I
R567	T1384	T1382	pobj	sheet,as
R5670	T8952	T8950	conj	6J,6I
R5671	T8953	T8947	punct	", ",exhibited
R5672	T8763	T8717	punct	.,took
R5673	T8954	T8955	preconj	both,WT
R5674	T8955	T8956	npadvmod	WT,null
R5675	T8765	T8766	prep	In,appeared
R5676	T8956	T8961	amod	null,buds
R5677	T8957	T8955	cc	and,WT
R5678	T8958	T8959	compound	TGF,β2
R5679	T8959	T8955	conj	β2,WT
R568	T1385	T1384	amod	single,sheet
R5680	T8960	T8959	punct	-,β2
R5681	T8767	T8765	pobj	contrast,In
R5682	T8961	T8947	nsubj	buds,exhibited
R5683	T8962	T8963	amod	nuclear,localization
R5684	T8963	T8947	dobj	localization,exhibited
R5685	T8964	T8963	nmod	LEF,localization
R5686	T8768	T8766	punct	", ",appeared
R5687	T8965	T8964	punct	-,LEF
R5688	T8966	T8964	nummod	1,LEF
R5689	T8967	T8964	cc	and,LEF
R569	T1386	T1384	prep	of,sheet
R5690	T8769	T8770	compound	TGF,β2
R5691	T8968	T8969	compound	β,catenin
R5692	T8969	T8964	conj	catenin,LEF
R5693	T8970	T8969	punct	-,catenin
R5694	T8982	T8947	punct	.,exhibited
R5695	T8971	T8947	punct	", ",exhibited
R5696	T8972	T8947	npadvmod	signs,exhibited
R5697	T8973	T8974	mark	that,was
R5698	T8974	T8972	acl	was,signs
R5699	T8975	T8976	det	the,pathway
R570	T1387	T1388	amod	multipotent,cells
R5700	T8976	T8974	nsubj	pathway,was
R5701	T8977	T8978	compound	Wnt,noggin
R5702	T8984	T8985	det	These,data
R5703	T8978	T8976	compound	noggin,pathway
R5704	T8979	T8978	punct	-,noggin
R5705	T8980	T8976	compound	signaling,pathway
R5706	T8981	T8974	acomp	intact,was
R5707	T8985	T8986	nsubj	data,suggest
R5708	T8987	T8988	mark	that,functions
R5709	T8988	T8986	ccomp	functions,suggest
R571	T1388	T1386	pobj	cells,of
R5710	T8989	T8988	prep	during,functions
R5711	T8990	T8991	compound	hair,follicle
R5712	T8991	T8992	compound	follicle,morphogenesis
R5713	T8992	T8989	pobj	morphogenesis,during
R5714	T8993	T8988	punct	", ",functions
R5715	T8994	T8995	compound	TGF,β2
R5716	T8995	T8988	nsubj	β2,functions
R5717	T8996	T8995	punct	-,β2
R5718	T8997	T8988	advmod	subsequently,functions
R5719	T8998	T8997	prep	to,subsequently
R572	T1389	T1388	amod	ectodermal,cells
R5720	T8999	T9000	compound	Wnt,noggin
R5721	T9000	T9002	npadvmod	noggin,mediated
R5722	T9001	T9000	punct	/,noggin
R5723	T9002	T9004	amod	mediated,determination
R5724	T9003	T9002	punct	-,mediated
R5725	T9004	T8998	pobj	determination,to
R5726	T9005	T9004	prep	of,determination
R5727	T9006	T9007	compound	hair,fate
R5728	T9007	T9005	pobj	fate,of
R5729	T9008	T8986	punct	.,suggest
R573	T1390	T1381	punct	.,begins
R5730	T9010	T9011	advmod	Moreover,appears
R5731	T9012	T9011	punct	", ",appears
R5732	T9013	T9011	prep	through,appears
R5733	T9014	T9013	pobj	activation,through
R5734	T9015	T9014	prep	of,activation
R5735	T9016	T9017	compound	Snail,expression
R5736	T9017	T9015	pobj	expression,of
R5737	T9018	T9017	compound	gene,expression
R5738	T9019	T9011	punct	", ",appears
R5739	T9020	T9021	compound	TGF,β2
R574	T1392	T1393	prep	During,populate
R5740	T9021	T9011	nsubj	β2,appears
R5741	T9022	T9021	punct	-,β2
R5742	T9023	T9024	aux	to,work
R5743	T9024	T9011	xcomp	work,appears
R5744	T9025	T9024	prep	in,work
R5745	T9026	T9025	pobj	tandem,in
R5746	T9027	T9026	prep	with,tandem
R5747	T9028	T9029	det	these,morphogens
R5748	T9029	T9027	pobj	morphogens,with
R5749	T9030	T9029	amod	other,morphogens
R575	T1394	T1392	pobj	development,During
R5750	T9031	T9032	aux	to,regulate
R5751	T9032	T9024	advcl	regulate,work
R5752	T9033	T9032	advmod	down,regulate
R5753	T9034	T9032	punct	-,regulate
R5754	T9035	T9036	compound	E,cadherin
R5755	T9036	T9038	compound	cadherin,levels
R5756	T9037	T9036	punct	-,cadherin
R5757	T9038	T9032	dobj	levels,regulate
R5758	T9039	T9024	punct	", ",work
R5759	T9040	T9041	dep	which,contributes
R576	T1395	T1393	punct	", ",populate
R5760	T9041	T9024	advcl	contributes,work
R5761	T9042	T9041	prep	to,contributes
R5762	T9043	T9044	det	the,activation
R5763	T9044	T9042	pobj	activation,to
R5764	T9045	T9044	prep	of,activation
R5765	T9046	T9047	amod	proliferative,circuitries
R5766	T9047	T9045	pobj	circuitries,of
R5767	T9048	T9011	punct	.,appears
R577	T1396	T1397	amod	specialized,cells
R5772	T9102	T9103	prep	During,govern
R5773	T9104	T9105	compound	budding,morphogenesis
R5774	T9105	T9102	pobj	morphogenesis,During
R5775	T9106	T9103	punct	", ",govern
R5776	T9107	T9108	amod	intersecting,networks
R5777	T9108	T9103	nsubj	networks,govern
R5778	T9109	T9108	compound	signaling,networks
R5779	T9110	T9108	prep	from,networks
R578	T1397	T1393	nsubj	cells,populate
R5780	T9111	T9112	det	the,epithelium
R5781	T9112	T9110	pobj	epithelium,from
R5782	T9113	T9112	cc	and,epithelium
R5783	T9114	T9112	conj	mesenchyme,epithelium
R5784	T9115	T9116	amod	transcriptional,programs
R5785	T9116	T9103	dobj	programs,govern
R5786	T9117	T9115	punct	", ",transcriptional
R5787	T9118	T9115	conj	adhesive,transcriptional
R5788	T9119	T9118	punct	", ",adhesive
R5789	T9120	T9118	conj	polarity,adhesive
R579	T1398	T1397	amod	mesenchymal,cells
R5790	T9121	T9120	punct	", ",polarity
R5791	T9122	T9120	cc	and,polarity
R5792	T9123	T9120	conj	motility,polarity
R5793	T9124	T9103	prep	in,govern
R5794	T9125	T9126	det	these,groups
R5795	T9126	T9124	pobj	groups,in
R5796	T9127	T9126	amod	select,groups
R5797	T9128	T9126	prep	of,groups
R5798	T9129	T9128	pobj	cells,of
R5799	T9130	T9103	punct	.,govern
R580	T1399	T1400	det	the,skin
R5800	T9132	T9133	det	The,changes
R5801	T9133	T9138	nsubj	changes,form
R5802	T9134	T9133	amod	dynamic,changes
R5803	T9135	T9133	amod	nuclear,changes
R5804	T9136	T9135	cc	and,nuclear
R5805	T9137	T9135	conj	cytosolic,nuclear
R5806	T9139	T9140	dep	that,take
R5807	T9140	T9133	relcl	take,changes
R5808	T9141	T9140	dobj	place,take
R5809	T9142	T9140	prep	during,take
R581	T1400	T1393	dobj	skin,populate
R5810	T9143	T9144	det	this,time
R5811	T9144	T9142	pobj	time,during
R5812	T9145	T9146	det	the,cornerstone
R5813	T9146	T9138	dobj	cornerstone,form
R5814	T9147	T9146	prep	for,cornerstone
R5815	T9148	T9149	compound	organ,morphogenesis
R5816	T9149	T9147	pobj	morphogenesis,for
R5817	T9150	T9138	punct	.,form
R5818	T9152	T9153	nummod	Two,challenges
R5819	T9153	T9155	nsubj	challenges,are
R582	T1401	T1393	prep	in,populate
R5820	T9154	T9153	amod	major,challenges
R5821	T9156	T9153	prep	in,challenges
R5822	T9157	T9156	pcomp	understanding,in
R5823	T9158	T9159	det	the,mechanisms
R5824	T9159	T9157	dobj	mechanisms,understanding
R5825	T9160	T9159	acl	underlying,mechanisms
R5826	T9161	T9162	det	a,process
R5827	T9162	T9160	dobj	process,underlying
R5828	T9163	T9162	amod	particular,process
R5829	T9164	T9162	compound	budding,process
R583	T1402	T1403	det	a,pattern
R5830	T9165	T9166	aux	to,order
R5831	T9166	T9155	xcomp	order,are
R5832	T9167	T9168	det	the,sequence
R5833	T9168	T9166	dobj	sequence,order
R5834	T9169	T9168	amod	temporal,sequence
R5835	T9170	T9168	prep	of,sequence
R5836	T9171	T9172	amod	external,cues
R5837	T9172	T9170	pobj	cues,of
R5838	T9173	T9172	acl	involved,cues
R5839	T9174	T9166	cc	and,order
R584	T1403	T1401	pobj	pattern,in
R5840	T9175	T9176	advmod	then,dissect
R5841	T9176	T9166	conj	dissect,order
R5842	T9177	T9176	aux	to,dissect
R5843	T9178	T9179	advmod	how,translate
R5844	T9179	T9176	ccomp	translate,dissect
R5845	T9180	T9181	det	the,cells
R5846	T9181	T9179	nsubj	cells,translate
R5847	T9182	T9181	prep	of,cells
R5848	T9183	T9184	det	the,bud
R5849	T9184	T9182	pobj	bud,of
R585	T1404	T1405	advmod	spatially,defined
R5850	T9185	T9184	amod	developing,bud
R5851	T9186	T9187	det	these,signals
R5852	T9187	T9179	dobj	signals,translate
R5853	T9188	T9179	prep	into,translate
R5854	T9189	T9190	det	the,events
R5855	T9190	T9188	pobj	events,into
R5856	T9191	T9190	amod	downstream,events
R5857	T9192	T9190	prep	of,events
R5858	T9193	T9194	amod	cellular,remodeling
R5859	T9194	T9192	pobj	remodeling,of
R586	T1405	T1403	amod	defined,pattern
R5860	T9195	T9194	punct	", ",remodeling
R5861	T9196	T9194	conj	proliferation,remodeling
R5862	T9197	T9196	punct	", ",proliferation
R5863	T9198	T9196	cc	and,proliferation
R5864	T9199	T9196	conj	differentiation,proliferation
R5865	T9200	T9155	punct	.,are
R5866	T9202	T9203	poss	Our,studies
R5867	T9203	T9204	nsubj	studies,provide
R5868	T9205	T9203	advmod	here,studies
R5869	T9206	T9207	det	some,insights
R587	T1406	T1407	aux	to,initiate
R5870	T9207	T9204	dobj	insights,provide
R5871	T9208	T9207	prep	into,insights
R5872	T9209	T9210	advmod	how,orchestrated
R5873	T9210	T9208	pcomp	orchestrated,into
R5874	T9211	T9212	det	these,events
R5875	T9212	T9210	nsubjpass	events,orchestrated
R5876	T9213	T9210	auxpass	are,orchestrated
R5877	T9214	T9210	prep	during,orchestrated
R5878	T9215	T9216	compound	hair,bud
R5879	T9216	T9217	compound	bud,formation
R588	T1407	T1393	advcl	initiate,populate
R5880	T9217	T9214	pobj	formation,during
R5881	T9218	T9210	prep	in,orchestrated
R5882	T9219	T9220	amod	developing,skin
R5883	T9220	T9218	pobj	skin,in
R5884	T9221	T9204	punct	.,provide
R589	T1408	T1409	det	the,crosstalk
R5895	T9654	T9653	prep	during,Signaling
R5896	T9655	T9656	amod	Early,Morphogenesis
R5897	T9656	T9654	pobj	Morphogenesis,during
R5898	T9657	T9658	compound	Hair,Follicle
R5899	T9658	T9656	compound	Follicle,Morphogenesis
R590	T1409	T1407	dobj	crosstalk,initiate
R5900	T9660	T9661	amod	Recent,studies
R5901	T9661	T9662	nsubj	studies,suggest
R5902	T9663	T9661	prep	on,studies
R5903	T9664	T9665	compound	hair,bud
R5904	T9665	T9666	compound	bud,morphogenesis
R5905	T9666	T9663	pobj	morphogenesis,on
R5906	T9667	T9668	mark	that,converge
R5907	T9668	T9662	ccomp	converge,suggest
R5908	T9669	T9670	compound	Wnt,signals
R5909	T9670	T9668	nsubj	signals,converge
R591	T1410	T1409	amod	complex,crosstalk
R5910	T9671	T9672	advmod	likely,from
R5911	T9672	T9670	prep	from,signals
R5912	T9673	T9674	det	the,epithelium
R5913	T9674	T9672	pobj	epithelium,from
R5914	T9675	T9670	cc	and,signals
R5915	T9676	T9677	nmod	BMP,signals
R5916	T9677	T9670	conj	signals,signals
R5917	T9678	T9677	amod	inhibitory,signals
R5918	T9679	T9677	prep	from,signals
R5919	T9680	T9681	det	the,mesenchyme
R592	T1411	T1412	amod	epithelial,mesenchymal
R5920	T9681	T9679	pobj	mesenchyme,from
R5921	T9682	T9681	amod	underlying,mesenchyme
R5922	T9683	T9684	aux	to,produce
R5923	T9684	T9668	advcl	produce,converge
R5924	T9685	T9686	det	an,complex
R5925	T9686	T9684	dobj	complex,produce
R5926	T9687	T9686	amod	active,complex
R5927	T9688	T9689	compound	transcription,factor
R5928	T9689	T9686	compound	factor,complex
R5929	T9690	T9686	acl	involving,complex
R593	T1412	T1409	amod	mesenchymal,crosstalk
R5930	T9691	T9692	compound	β,catenin
R5931	T9692	T9690	dobj	catenin,involving
R5932	T9693	T9692	punct	-,catenin
R5933	T9694	T9692	cc	and,catenin
R5934	T9695	T9692	conj	LEF,catenin
R5935	T9696	T9695	punct	-,LEF
R5936	T9697	T9695	nummod	1,LEF
R5937	T9698	T9686	punct	", ",complex
R5938	T9699	T9700	dep	which,plays
R5939	T9700	T9686	relcl	plays,complex
R594	T1413	T1412	punct	-,mesenchymal
R5940	T9701	T9700	prep	in,plays
R5941	T9702	T9701	pobj	turn,in
R5942	T9703	T9704	det	a,role
R5943	T9704	T9700	dobj	role,plays
R5944	T9705	T9704	amod	key,role
R5945	T9706	T9700	prep	in,plays
R5946	T9707	T9706	pcomp	specifying,in
R5947	T9708	T9709	det	the,fate
R5948	T9709	T9707	dobj	fate,specifying
R5949	T9710	T9711	compound	hair,follicle
R595	T1414	T1415	dep	that,specify
R5950	T9711	T9709	compound	follicle,fate
R5951	T9712	T9713	punct	[,37
R5952	T9713	T9684	parataxis	37,produce
R5953	T9714	T9713	nummod	4,37
R5954	T9715	T9713	punct	",",37
R5955	T9716	T9713	nummod	29,37
R5956	T9717	T9713	punct	",",37
R5957	T9718	T9713	nummod	30,37
R5958	T9719	T9713	punct	",",37
R5959	T9720	T9713	nummod	36,37
R596	T1415	T1409	relcl	specify,crosstalk
R5960	T9721	T9713	punct	",",37
R5961	T9722	T9713	punct	],37
R5962	T9723	T9662	punct	.,suggest
R5963	T9725	T9726	amod	Sonic,hedgehog
R5964	T9726	T9727	nsubj	hedgehog,play
R5965	T9728	T9726	punct	(,hedgehog
R5966	T9729	T9726	appos	Shh,hedgehog
R5967	T9730	T9726	punct	),hedgehog
R5968	T9731	T9726	cc	and,hedgehog
R5969	T9732	T9733	compound	TGF,β2
R597	T1416	T1415	aux	will,specify
R5970	T9733	T9735	compound	β2,signaling
R5971	T9734	T9733	punct	-,β2
R5972	T9735	T9726	conj	signaling,hedgehog
R5973	T9736	T9727	advmod	also,play
R5974	T9737	T9738	amod	essential,roles
R5975	T9738	T9727	dobj	roles,play
R5976	T9739	T9727	prep	in,play
R5977	T9740	T9741	compound	follicle,morphogenesis
R5978	T9741	T9739	pobj	morphogenesis,in
R5979	T9742	T9727	punct	", ",play
R598	T1417	T1418	det	the,bud
R5980	T9743	T9727	cc	but,play
R5981	T9744	T9745	prep	in,form
R5982	T9745	T9727	conj	form,play
R5983	T9746	T9744	pobj	contrast,in
R5984	T9747	T9746	prep	to,contrast
R5985	T9748	T9749	compound	β,catenin
R5986	T9749	T9751	npadvmod	catenin,null
R5987	T9750	T9749	punct	-,catenin
R5988	T9751	T9752	amod	null,skin
R5989	T9752	T9747	pobj	skin,to
R599	T1418	T1415	dobj	bud,specify
R5990	T9753	T9752	punct	", ",skin
R5991	T9754	T9755	prep	in,are
R5992	T9755	T9752	relcl	are,skin
R5993	T9756	T9754	pobj	which,in
R5994	T9757	T9758	compound	follicle,invaginations
R5995	T9758	T9755	nsubj	invaginations,are
R5996	T9759	T9755	acomp	absent,are
R5997	T9760	T9761	punct	[,30
R5998	T9761	T9747	parataxis	30,to
R5999	T9762	T9761	punct	],30
R600	T1419	T1420	punct	[,3
R6000	T9763	T9745	punct	", ",form
R6001	T9764	T9765	det	some,buds
R6002	T9765	T9745	nsubj	buds,form
R6003	T9766	T9765	compound	hair,buds
R6004	T9767	T9745	advmod	still,form
R6005	T9768	T9745	prep	in,form
R6006	T9769	T9770	det	the,absence
R6007	T9770	T9768	pobj	absence,in
R6008	T9771	T9770	prep	of,absence
R6009	T9772	T9771	pobj	LEF,of
R601	T1420	T1393	parataxis	3,populate
R6010	T9773	T9772	punct	-,LEF
R6011	T9774	T9772	nummod	1,LEF
R6012	T9775	T9772	punct	", ",LEF
R6013	T9776	T9772	conj	Shh,LEF
R6014	T9777	T9776	punct	", ",Shh
R6015	T9778	T9776	cc	or,Shh
R6016	T9779	T9780	compound	TGF,β2
R6017	T9780	T9776	conj	β2,Shh
R6018	T9781	T9780	punct	-,β2
R6019	T9782	T9783	punct	[,38
R602	T1421	T1420	punct	],3
R6020	T9783	T9745	parataxis	38,form
R6021	T9784	T9783	nummod	32,38
R6022	T9785	T9783	punct	",",38
R6023	T9786	T9783	punct	],38
R6024	T9787	T9745	punct	.,form
R6025	T9789	T9790	nsubj	These,reflect
R6026	T9791	T9790	advmod	likely,reflect
R6027	T9792	T9793	det	the,wave
R6028	T9793	T9790	dobj	wave,reflect
R6029	T9794	T9793	amod	first,wave
R603	T1422	T1393	punct	.,populate
R6030	T9795	T9793	prep	of,wave
R6031	T9796	T9797	nmod	follicle,morphogenesis
R6032	T9797	T9795	pobj	morphogenesis,of
R6033	T9798	T9799	punct	(,hair
R6034	T9799	T9797	parataxis	hair,morphogenesis
R6035	T9800	T9799	advmod	i.e.,hair
R6036	T9801	T9799	punct	", ",hair
R6037	T9802	T9799	compound	guard,hair
R6038	T9803	T9799	punct	),hair
R6039	T9804	T9793	punct	", ",wave
R604	T1424	T1425	mark	Once,committed
R6040	T9805	T9806	dep	which,accounts
R6041	T9806	T9793	relcl	accounts,wave
R6042	T9807	T9806	prep	for,accounts
R6043	T9808	T9809	det	a,number
R6044	T9809	T9807	pobj	number,for
R6045	T9810	T9809	amod	small,number
R6046	T9811	T9809	punct	(,number
R6047	T9812	T9813	amod	fewer,5
R6048	T9813	T9815	nummod	5,%
R6049	T9814	T9813	quantmod	than,5
R605	T1425	T1426	advcl	committed,instructs
R6050	T9815	T9809	appos	%,number
R6051	T9816	T9809	punct	),number
R6052	T9817	T9809	prep	of,number
R6053	T9818	T9817	pobj	hairs,of
R6054	T9819	T9806	cc	and,accounts
R6055	T9820	T9806	conj	is,accounts
R6056	T9821	T9820	prep	under,is
R6057	T9822	T9823	amod	distinct,control
R6058	T9823	T9821	pobj	control,under
R6059	T9824	T9823	amod	regulatory,control
R606	T1427	T1426	punct	", ",instructs
R6060	T9825	T9790	punct	.,reflect
R6061	T9827	T9828	compound	Guard,hairs
R6062	T9828	T9829	nsubj	hairs,form
R6063	T9830	T9829	prep	in,form
R6064	T9831	T9832	det	the,absence
R6065	T9832	T9830	pobj	absence,in
R6066	T9833	T9832	prep	of,absence
R6067	T9834	T9833	pobj	LEF,of
R6068	T9835	T9834	punct	-,LEF
R6069	T9836	T9834	nummod	1,LEF
R607	T1428	T1429	det	a,cluster
R6070	T9837	T9834	cc	and,LEF
R6071	T9838	T9839	compound	TGF,β2
R6072	T9839	T9834	conj	β2,LEF
R6073	T9840	T9839	punct	-,β2
R6074	T9841	T9829	punct	", ",form
R6075	T9842	T9829	cc	and,form
R6076	T9843	T9844	nsubj	we,found
R6077	T9844	T9829	conj	found,form
R6078	T9845	T9844	aux	have,found
R6079	T9846	T9847	mark	that,fail
R608	T1429	T1426	nsubj	cluster,instructs
R6080	T9847	T9844	ccomp	fail,found
R6081	T9848	T9847	nsubj	they,fail
R6082	T9849	T9847	advmod	also,fail
R6083	T9850	T9851	aux	to,express
R6084	T9851	T9847	xcomp	express,fail
R6085	T9852	T9851	dobj	Snail,express
R6086	T9853	T9847	prep	at,fail
R6087	T9854	T9855	det	the,stage
R6088	T9855	T9853	pobj	stage,at
R6089	T9856	T9855	compound	budding,stage
R609	T1430	T1429	amod	small,cluster
R6090	T9857	T9855	prep	of,stage
R6091	T9858	T9857	pobj	development,of
R6092	T9859	T9860	punct	(,data
R6093	T9860	T9844	meta	data,found
R6094	T9861	T9860	amod	unpublished,data
R6095	T9862	T9860	punct	),data
R6096	T9863	T9844	punct	.,found
R6097	T9865	T9866	advmod	How,regulated
R6098	T9866	T9871	csubj	regulated,remains
R6099	T9867	T9868	compound	E,cadherin
R610	T1431	T1429	prep	of,cluster
R6100	T9868	T9866	nsubjpass	cadherin,regulated
R6101	T9869	T9868	punct	-,cadherin
R6102	T9870	T9866	auxpass	is,regulated
R6103	T9872	T9866	prep	in,regulated
R6104	T9873	T9874	compound	guard,hairs
R6105	T9874	T9872	pobj	hairs,in
R6106	T9875	T9876	aux	to,determined
R6107	T9876	T9871	xcomp	determined,remains
R6108	T9877	T9876	auxpass	be,determined
R6109	T9878	T9871	punct	.,remains
R611	T1432	T1433	amod	epithelial,cells
R6110	T9880	T9881	amod	Several,candidates
R6111	T9881	T9882	nsubj	candidates,include
R6112	T9883	T9884	amod	other,members
R6113	T9884	T9882	dobj	members,include
R6114	T9885	T9884	compound	Snail,members
R6115	T9886	T9884	compound	family,members
R6116	T9887	T9888	amod	such,as
R6117	T9888	T9884	prep	as,members
R6118	T9889	T9888	pobj	Slug,as
R6119	T9890	T9889	cc	or,Slug
R612	T1433	T1431	pobj	cells,of
R6120	T9891	T9889	conj	twist,Slug
R6121	T9892	T9884	punct	", ",members
R6122	T9893	T9884	cc	or,members
R6123	T9894	T9895	advmod	alternatively,factors
R6124	T9895	T9884	conj	factors,members
R6125	T9896	T9895	punct	", ",factors
R6126	T9897	T9895	compound	transcription,factors
R6127	T9898	T9895	acl	involving,factors
R6128	T9899	T9900	compound	β,catenin
R6129	T9900	T9898	dobj	catenin,involving
R613	T1434	T1429	punct	", ",cluster
R6130	T9901	T9900	punct	-,catenin
R6131	T9902	T9900	cc	and,catenin
R6132	T9903	T9904	det	a,member
R6133	T9904	T9900	conj	member,catenin
R6134	T9905	T9904	amod	different,member
R6135	T9906	T9904	prep	of,member
R6136	T9907	T9908	det	the,family
R6137	T9908	T9906	pobj	family,of
R6138	T9909	T9910	nmod	LEF,box
R6139	T9910	T9908	nmod	box,family
R614	T1435	T1436	det	the,placode
R6140	T9911	T9909	punct	-,LEF
R6141	T9912	T9909	nummod	1,LEF
R6142	T9913	T9909	punct	/,LEF
R6143	T9914	T9909	appos	TCF,LEF
R6144	T9915	T9909	punct	/,LEF
R6145	T9916	T9917	compound	Sry,type
R6146	T9917	T9909	appos	type,LEF
R6147	T9918	T9917	punct	-,type
R6148	T9919	T9910	nmod	HMG,box
R6149	T9920	T9921	punct	(,known
R615	T1436	T1429	appos	placode,cluster
R6150	T9921	T9910	parataxis	known,box
R6151	T9922	T9921	advmod	commonly,known
R6152	T9923	T9921	prep	as,known
R6153	T9924	T9923	pobj	SOX,as
R6154	T9925	T9921	punct	),known
R6155	T9926	T9927	punct	[,40
R6156	T9927	T9882	parataxis	40,include
R6157	T9928	T9927	nummod	39,40
R6158	T9929	T9927	punct	",",40
R6159	T9930	T9927	punct	],40
R616	T1437	T1426	punct	", ",instructs
R6160	T9931	T9882	punct	.,include
R6161	T9933	T9934	amod	Further,investigation
R6162	T9934	T9935	nsubjpass	investigation,required
R6163	T9936	T9935	aux	will,required
R6164	T9937	T9935	auxpass	be,required
R6165	T9938	T9939	aux	to,determine
R6166	T9939	T9935	advcl	determine,required
R6167	T9940	T9941	mark	whether,is
R6168	T9941	T9939	ccomp	is,determine
R6169	T9942	T9943	det	the,pathway
R617	T1438	T1439	det	a,group
R6170	T9943	T9941	nsubj	pathway,is
R6171	T9944	T9943	compound	signaling,pathway
R6172	T9945	T9946	nsubj	we,elucidated
R6173	T9946	T9943	advcl	elucidated,pathway
R6174	T9947	T9946	aux	have,elucidated
R6175	T9948	T9946	advmod	here,elucidated
R6176	T9949	T9950	det	a,theme
R6177	T9950	T9941	attr	theme,is
R6178	T9951	T9950	prep	with,theme
R6179	T9952	T9953	amod	multiple,variations
R618	T1439	T1440	nsubj	group,condense
R6180	T9953	T9951	pobj	variations,with
R6181	T9954	T9935	punct	.,required
R6182	T9956	T9957	compound	TGF,βs
R6183	T9957	T9959	nsubjpass	βs,known
R6184	T9958	T9957	punct	-,βs
R6185	T9960	T9959	auxpass	are,known
R6186	T9961	T9962	aux	to,promote
R6187	T9962	T9959	xcomp	promote,known
R6188	T9963	T9962	dobj	withdrawal,promote
R6189	T9964	T9963	prep	of,withdrawal
R619	T1440	T1426	ccomp	condense,instructs
R6190	T9965	T9964	pobj	keratinocytes,of
R6191	T9966	T9963	prep	from,withdrawal
R6192	T9967	T9968	det	the,cycle
R6193	T9968	T9966	pobj	cycle,from
R6194	T9969	T9968	compound	cell,cycle
R6195	T9970	T9971	punct	[,41
R6196	T9971	T9959	parataxis	41,known
R6197	T9972	T9971	punct	],41
R6198	T9973	T9959	punct	.,known
R6199	T9975	T9976	advmod	Hence,focused
R620	T1441	T1439	prep	of,group
R6200	T9977	T9976	punct	", ",focused
R6201	T9978	T9979	advmod	when,detected
R6202	T9979	T9976	advcl	detected,focused
R6203	T9980	T9981	compound	TGF,β2
R6204	T9981	T9983	compound	β2,protein
R6205	T9982	T9981	punct	-,β2
R6206	T9983	T9979	nsubjpass	protein,detected
R6207	T9984	T9979	auxpass	was,detected
R6208	T9985	T9979	prep	at,detected
R6209	T9986	T9987	det	the,transition
R621	T1442	T1443	amod	underlying,cells
R6210	T9987	T9985	pobj	transition,at
R6211	T9988	T9987	prep	between,transition
R6212	T9989	T9990	det	the,phases
R6213	T9990	T9988	pobj	phases,between
R6214	T9991	T9992	npadvmod	growing,destructive
R6215	T9992	T9990	amod	destructive,phases
R6216	T9993	T9992	cc	and,destructive
R6217	T9994	T9990	prep	of,phases
R6218	T9995	T9996	det	the,cycle
R6219	T9996	T9994	pobj	cycle,of
R622	T1443	T1441	pobj	cells,of
R6220	T9997	T9996	amod	adult,cycle
R6221	T9998	T9996	compound	hair,cycle
R6222	T9999	T9976	punct	", ",focused
R6223	T10000	T9976	nsubj	research,focused
R6224	T10001	T9976	advmod	initially,focused
R6225	T10002	T10001	cc	and,initially
R6226	T10003	T10001	conj	naturally,initially
R6227	T10004	T9976	prep	on,focused
R6228	T10005	T10006	det	a,role
R6229	T10006	T10004	pobj	role,on
R623	T1444	T1443	amod	mesenchymal,cells
R6230	T10007	T10006	prep	for,role
R6231	T10008	T10009	det	this,member
R6232	T10009	T10007	pobj	member,for
R6233	T10010	T10009	compound	family,member
R6234	T10011	T10006	prep	in,role
R6235	T10012	T10011	pobj	cessation,in
R6236	T10013	T10012	prep	of,cessation
R6237	T10014	T10013	pobj	growth,of
R6238	T10015	T10012	cc	and,cessation
R6239	T10016	T10015	punct	/,and
R624	T1445	T1440	aux	to,condense
R6240	T10017	T10015	cc	or,and
R6241	T10018	T10012	conj	triggering,cessation
R6242	T10019	T10018	dobj	apoptosis,triggering
R6243	T10020	T10021	punct	(,42
R6244	T10021	T9976	parataxis	42,focused
R6245	T10022	T10021	punct	[,42
R6246	T10023	T10021	punct	],42
R6247	T10024	T10021	cc	and,42
R6248	T10025	T10021	conj	references,42
R6249	T10026	T10025	advmod	therein,references
R625	T1446	T1440	cc	and,condense
R6250	T10027	T10021	punct	),42
R6251	T10028	T9976	punct	.,focused
R6252	T10030	T10031	advmod	However,displays
R6253	T10032	T10031	punct	", ",displays
R6254	T10033	T10031	prep	in,displays
R6255	T10034	T10033	pobj	contrast,in
R6256	T10035	T10034	prep	to,contrast
R6257	T10036	T10037	compound	TGF,β1
R6258	T10037	T10039	npadvmod	β1,null
R6259	T10038	T10037	punct	-,β1
R626	T1447	T1440	conj	form,condense
R6260	T10039	T10041	amod	null,skin
R6261	T10040	T10039	punct	-,null
R6262	T10041	T10035	pobj	skin,to
R6263	T10042	T10041	punct	", ",skin
R6264	T10043	T10044	dep	which,exhibits
R6265	T10044	T10041	relcl	exhibits,skin
R6266	T10045	T10046	det	an,phase
R6267	T10046	T10044	dobj	phase,exhibits
R6268	T10047	T10046	amod	extended,phase
R6269	T10048	T10046	compound	growing,phase
R627	T1448	T1449	det	the,papilla
R6270	T10049	T10046	prep	of,phase
R6271	T10050	T10051	amod	postnatal,follicles
R6272	T10051	T10049	pobj	follicles,of
R6273	T10052	T10051	compound	hair,follicles
R6274	T10053	T10031	punct	", ",displays
R6275	T10054	T10055	compound	TGF,β2
R6276	T10055	T10057	npadvmod	β2,null
R6277	T10056	T10055	punct	-,β2
R6278	T10057	T10059	amod	null,skin
R6279	T10058	T10057	punct	-,null
R628	T1449	T1447	dobj	papilla,form
R6280	T10059	T10031	nsubj	skin,displays
R6281	T10060	T10061	det	an,block
R6282	T10061	T10031	dobj	block,displays
R6283	T10062	T10061	amod	embryonic,block
R6284	T10063	T10061	prep	in,block
R6285	T10064	T10065	compound	follicle,bud
R6286	T10065	T10066	compound	bud,progression
R6287	T10066	T10063	pobj	progression,in
R6288	T10067	T10068	punct	[,32
R6289	T10068	T10031	parataxis	32,displays
R629	T1450	T1449	amod	nascent,papilla
R6290	T10069	T10068	punct	],32
R6291	T10070	T10031	punct	.,displays
R6292	T10072	T10073	mark	Although,is
R6293	T10073	T10076	advcl	is,appear
R6294	T10074	T10075	det	this,phenotype
R6295	T10075	T10073	nsubj	phenotype,is
R6296	T10077	T10073	acomp	consistent,is
R6297	T10078	T10077	prep	with,consistent
R6298	T10079	T10080	compound	TGF,β2
R6299	T10080	T10082	poss	β2,patterns
R630	T1451	T1449	amod	dermal,papilla
R6300	T10081	T10080	punct	-,β2
R6301	T10082	T10078	pobj	patterns,with
R6302	T10083	T10080	case	's,β2
R6303	T10084	T10082	amod	embryonic,patterns
R6304	T10085	T10082	compound	expression,patterns
R6305	T10086	T10087	punct	[,33
R6306	T10087	T10073	parataxis	33,is
R6307	T10088	T10087	punct	],33
R6308	T10089	T10076	punct	", ",appear
R6309	T10090	T10091	quantmod	about,50
R631	T1452	T1449	punct	", ",papilla
R6310	T10091	T10092	nummod	50,%
R6311	T10092	T10076	nsubj	%,appear
R6312	T10093	T10092	prep	of,%
R6313	T10094	T10095	compound	TGF,β2
R6314	T10095	T10097	npadvmod	β2,null
R6315	T10096	T10095	punct	-,β2
R6316	T10097	T10098	amod	null,buds
R6317	T10098	T10093	pobj	buds,of
R6318	T10099	T10076	oprd	unable,appear
R6319	T10100	T10101	aux	to,progress
R632	T1453	T1454	dep	which,be
R6320	T10101	T10099	xcomp	progress,unable
R6321	T10102	T10101	prep	to,progress
R6322	T10103	T10104	det	the,phase
R6323	T10104	T10102	pobj	phase,to
R6324	T10105	T10106	amod	down,growth
R6325	T10106	T10104	compound	growth,phase
R6326	T10107	T10106	punct	-,growth
R6327	T10108	T10099	punct	", ",unable
R6328	T10109	T10110	det	a,feature
R6329	T10110	T10099	npadvmod	feature,unable
R633	T1454	T1449	relcl	be,papilla
R6330	T10111	T10112	dep	that,explained
R6331	T10112	T10110	relcl	explained,feature
R6332	T10113	T10112	aux	can,explained
R6333	T10114	T10112	neg	not,explained
R6334	T10115	T10112	auxpass	be,explained
R6335	T10116	T10112	advmod	readily,explained
R6336	T10117	T10112	prep	on,explained
R6337	T10118	T10119	det	the,basis
R6338	T10119	T10117	pobj	basis,on
R6339	T10120	T10119	prep	of,basis
R634	T1455	T1454	aux	will,be
R6340	T10121	T10122	advmod	previously,established
R6341	T10122	T10123	amod	established,effects
R6342	T10123	T10120	pobj	effects,of
R6343	T10124	T10123	prep	of,effects
R6344	T10125	T10126	compound	TGF,βs
R6345	T10126	T10124	pobj	βs,of
R6346	T10127	T10126	punct	-,βs
R6347	T10128	T10076	punct	.,appear
R6348	T10130	T10131	poss	Our,finding
R6349	T10131	T10132	nsubj	finding,lends
R635	T1456	T1457	det	a,fixture
R6350	T10133	T10134	mark	that,is
R6351	T10134	T10131	acl	is,finding
R6352	T10135	T10136	compound	TGF,β2
R6353	T10136	T10134	nsubj	β2,is
R6354	T10137	T10136	punct	-,β2
R6355	T10138	T10134	advmod	upstream,is
R6356	T10139	T10138	prep	from,upstream
R6357	T10140	T10141	compound	Ki67,expression
R6358	T10141	T10139	pobj	expression,from
R6359	T10142	T10141	cc	and,expression
R636	T1457	T1454	attr	fixture,be
R6360	T10143	T10144	compound	MAPK,activation
R6361	T10144	T10141	conj	activation,expression
R6362	T10145	T10146	amod	further,support
R6363	T10146	T10132	dobj	support,lends
R6364	T10147	T10132	dative	to,lends
R6365	T10148	T10149	det	the,notion
R6366	T10149	T10147	pobj	notion,to
R6367	T10150	T10151	mark	that,react
R6368	T10151	T10149	acl	react,notion
R6369	T10152	T10153	compound	hair,follicle
R637	T1458	T1457	amod	permanent,fixture
R6370	T10153	T10154	compound	follicle,keratinocytes
R6371	T10154	T10151	nsubj	keratinocytes,react
R6372	T10155	T10154	prep	at,keratinocytes
R6373	T10156	T10157	det	this,stage
R6374	T10157	T10155	pobj	stage,at
R6375	T10158	T10157	amod	early,stage
R6376	T10159	T10157	prep	of,stage
R6377	T10160	T10159	pobj	development,of
R6378	T10161	T10151	prep	to,react
R6379	T10162	T10163	compound	TGF,β2
R638	T1459	T1457	prep	of,fixture
R6380	T10163	T10165	compound	β2,signaling
R6381	T10164	T10163	punct	-,β2
R6382	T10165	T10161	pobj	signaling,to
R6383	T10166	T10151	prep	in,react
R6384	T10167	T10168	det	a,fashion
R6385	T10168	T10166	pobj	fashion,in
R6386	T10169	T10168	amod	opposite,fashion
R6387	T10170	T10169	prep	to,opposite
R6388	T10171	T10170	pobj	that,to
R6389	T10172	T10173	advmod	typically,expected
R639	T1460	T1461	det	the,follicle
R6390	T10173	T10171	acl	expected,that
R6391	T10174	T10173	prep	for,expected
R6392	T10175	T10176	compound	TGF,β
R6393	T10176	T10178	compound	β,factors
R6394	T10177	T10176	punct	-,β
R6395	T10178	T10174	pobj	factors,for
R6396	T10179	T10132	punct	.,lends
R6397	T10181	T10182	nsubj	This,said
R6398	T10182	T10183	advcl	said,appeared
R6399	T10184	T10183	punct	", ",appeared
R640	T1461	T1459	pobj	follicle,of
R6400	T10185	T10183	prep	based,appeared
R6401	T10186	T10185	prep	upon,based
R6402	T10187	T10188	compound	pSMAD2,immunohistochemistry
R6403	T10188	T10186	pobj	immunohistochemistry,upon
R6404	T10189	T10183	punct	", ",appeared
R6405	T10190	T10191	det	the,steps
R6406	T10191	T10183	nsubj	steps,appeared
R6407	T10192	T10191	amod	immediate,steps
R6408	T10193	T10191	prep	of,steps
R6409	T10194	T10195	amod	downstream,signaling
R641	T1462	T1461	compound	hair,follicle
R6410	T10195	T10193	pobj	signaling,of
R6411	T10196	T10197	aux	to,be
R6412	T10197	T10183	xcomp	be,appeared
R6413	T10198	T10197	acomp	intact,be
R6414	T10199	T10183	punct	.,appeared
R6415	T10201	T10202	advmod	Thus,surmise
R6416	T10203	T10202	punct	", ",surmise
R6417	T10204	T10202	nsubj	we,surmise
R6418	T10205	T10206	mark	that,is
R6419	T10206	T10202	ccomp	is,surmise
R642	T1463	T1426	punct	.,instructs
R6420	T10207	T10208	det	the,outcome
R6421	T10208	T10206	nsubj	outcome,is
R6422	T10209	T10208	amod	proliferative,outcome
R6423	T10210	T10206	acomp	likely,is
R6424	T10211	T10212	aux	to,rooted
R6425	T10212	T10210	xcomp	rooted,likely
R6426	T10213	T10212	auxpass	be,rooted
R6427	T10214	T10212	prep	in,rooted
R6428	T10215	T10214	pobj	differences,in
R6429	T10216	T10215	prep	in,differences
R643	T1465	T1466	amod	Subsequent,exchanges
R6430	T10217	T10218	det	the,repertoire
R6431	T10218	T10216	pobj	repertoire,in
R6432	T10219	T10218	prep	of,repertoire
R6433	T10220	T10221	amod	activated,genes
R6434	T10221	T10219	pobj	genes,of
R6435	T10222	T10221	compound	SMAD,genes
R6436	T10223	T10221	compound	target,genes
R6437	T10224	T10202	punct	.,surmise
R6438	T10226	T10227	prep	In,be
R6439	T10228	T10229	det	this,regard
R644	T1466	T1467	nsubj	exchanges,result
R6440	T10229	T10226	pobj	regard,In
R6441	T10230	T10227	punct	", ",be
R6442	T10231	T10232	det	the,effects
R6443	T10232	T10227	nsubj	effects,be
R6444	T10233	T10232	amod	positive,effects
R6445	T10234	T10232	prep	of,effects
R6446	T10235	T10236	compound	TGF,β2
R6447	T10236	T10234	pobj	β2,of
R6448	T10237	T10236	punct	-,β2
R6449	T10238	T10232	prep	on,effects
R645	T1468	T1466	prep	between,exchanges
R6450	T10239	T10238	pobj	proliferation,on
R6451	T10240	T10232	prep	within,effects
R6452	T10241	T10242	det	the,bud
R6453	T10242	T10240	pobj	bud,within
R6454	T10243	T10242	compound	hair,bud
R6455	T10244	T10227	aux	may,be
R6456	T10245	T10246	advmod	more,analogous
R6457	T10246	T10227	acomp	analogous,be
R6458	T10247	T10246	prep	to,analogous
R6459	T10248	T10249	dep	what,seen
R646	T1469	T1470	det	the,placode
R6460	T10249	T10247	pobj	seen,to
R6461	T10250	T10249	aux	has,seen
R6462	T10251	T10249	auxpass	been,seen
R6463	T10252	T10249	prep	in,seen
R6464	T10253	T10252	pobj	progression,in
R6465	T10254	T10253	prep	of,progression
R6466	T10255	T10256	amod	squamous,cell
R6467	T10256	T10257	compound	cell,carcinoma
R6468	T10257	T10254	pobj	carcinoma,of
R6469	T10258	T10253	prep	to,progression
R647	T1470	T1468	pobj	placode,between
R6470	T10259	T10260	amod	metastatic,carcinoma
R6471	T10260	T10258	pobj	carcinoma,to
R6472	T10261	T10262	punct	[,43
R6473	T10262	T10249	parataxis	43,seen
R6474	T10263	T10262	punct	],43
R6475	T10264	T10265	advmod	rather,than
R6476	T10265	T10249	cc	than,seen
R6477	T10266	T10249	conj	that,seen
R6478	T10267	T10268	advmod	typically,observed
R6479	T10268	T10266	acl	observed,that
R648	T1471	T1470	cc	and,placode
R6480	T10269	T10268	prep	for,observed
R6481	T10270	T10269	pobj	keratinocytes,for
R6482	T10271	T10272	punct	[,46
R6483	T10272	T10227	parataxis	46,be
R6484	T10273	T10272	nummod	44,46
R6485	T10274	T10272	punct	",",46
R6486	T10275	T10272	nummod	45,46
R6487	T10276	T10272	punct	",",46
R6488	T10277	T10272	punct	],46
R6489	T10278	T10227	punct	.,be
R649	T1472	T1473	amod	nascent,papilla
R6490	T10280	T10281	det	The,identification
R6491	T10281	T10283	nsubj	identification,was
R6492	T10282	T10281	amod	prior,identification
R6493	T10284	T10281	prep	of,identification
R6494	T10285	T10286	det	the,gene
R6495	T10286	T10284	pobj	gene,of
R6496	T10287	T10286	compound	Snail,gene
R6497	T10288	T10281	prep	as,identification
R6498	T10289	T10290	det	a,target
R6499	T10290	T10288	pobj	target,as
R650	T1473	T1470	conj	papilla,placode
R6500	T10291	T10290	amod	potential,target
R6501	T10292	T10290	prep	of,target
R6502	T10293	T10294	compound	TGF,β
R6503	T10294	T10296	compound	β,signaling
R6504	T10295	T10294	punct	-,β
R6505	T10296	T10292	pobj	signaling,of
R6506	T10297	T10298	punct	[,15
R6507	T10298	T10281	parataxis	15,identification
R6508	T10299	T10298	punct	],15
R6509	T10300	T10283	acomp	intriguing,was
R651	T1474	T1473	amod	dermal,papilla
R6510	T10301	T10283	punct	", ",was
R6511	T10302	T10283	prep	given,was
R6512	T10303	T10304	det	the,wave
R6513	T10304	T10302	pobj	wave,given
R6514	T10305	T10304	amod	temporal,wave
R6515	T10306	T10304	prep	of,wave
R6516	T10307	T10308	compound	Snail,expression
R6517	T10308	T10306	pobj	expression,of
R6518	T10309	T10308	compound	gene,expression
R6519	T10310	T10311	dep	that,occurs
R652	T1475	T1467	prep	in,result
R6520	T10311	T10304	relcl	occurs,wave
R6521	T10312	T10311	prep	in,occurs
R6522	T10313	T10314	det	the,bud
R6523	T10314	T10312	pobj	bud,in
R6524	T10315	T10314	amod	developing,bud
R6525	T10316	T10314	compound	hair,bud
R6526	T10317	T10283	punct	.,was
R6527	T10319	T10320	det	The,correlation
R6528	T10320	T10322	nsubj	correlation,fostered
R6529	T10321	T10320	amod	additional,correlation
R653	T1476	T1477	amod	further,growth
R6530	T10323	T10320	prep	between,correlation
R6531	T10324	T10325	amod	epithelial,hyperproliferation
R6532	T10325	T10323	pobj	hyperproliferation,between
R6533	T10326	T10325	cc	and,hyperproliferation
R6534	T10327	T10328	compound	Snail,expression
R6535	T10328	T10325	conj	expression,hyperproliferation
R6536	T10329	T10328	compound	transgene,expression
R6537	T10330	T10322	advmod	further,fostered
R6538	T10331	T10332	poss	our,interest
R6539	T10332	T10322	dobj	interest,fostered
R654	T1477	T1475	pobj	growth,in
R6540	T10333	T10332	prep	in,interest
R6541	T10334	T10335	det	a,link
R6542	T10335	T10333	pobj	link,in
R6543	T10336	T10335	amod	possible,link
R6544	T10337	T10335	prep	between,link
R6545	T10338	T10339	compound	TGF,β2
R6546	T10339	T10337	pobj	β2,between
R6547	T10340	T10339	punct	-,β2
R6548	T10341	T10339	cc	and,β2
R6549	T10342	T10339	conj	Snail,β2
R655	T1478	T1477	prep	of,growth
R6550	T10343	T10322	punct	.,fostered
R6551	T10345	T10346	poss	Our,studies
R6552	T10346	T10348	nsubj	studies,demonstrate
R6553	T10347	T10346	amod	functional,studies
R6554	T10349	T10350	mark	that,abolished
R6555	T10350	T10348	ccomp	abolished,demonstrate
R6556	T10351	T10350	prep	without,abolished
R6557	T10352	T10353	compound	TGF,β2
R6558	T10353	T10351	pobj	β2,without
R6559	T10354	T10353	punct	-,β2
R656	T1479	T1480	det	the,follicle
R6560	T10355	T10350	punct	", ",abolished
R6561	T10356	T10357	compound	Snail,expression
R6562	T10357	T10350	nsubjpass	expression,abolished
R6563	T10358	T10350	auxpass	is,abolished
R6564	T10359	T10350	prep	in,abolished
R6565	T10360	T10361	det	the,buds
R6566	T10361	T10359	pobj	buds,in
R6567	T10362	T10361	compound	mutant,buds
R6568	T10363	T10361	compound	hair,buds
R6569	T10364	T10350	punct	", ",abolished
R657	T1480	T1478	pobj	follicle,of
R6570	T10365	T10350	cc	and,abolished
R6571	T10366	T10367	advmod	conversely,activated
R6572	T10367	T10350	conj	activated,abolished
R6573	T10368	T10367	punct	", ",activated
R6574	T10369	T10367	prep	in,activated
R6575	T10370	T10371	compound	K14,Smad2
R6576	T10371	T10373	compound	Smad2,skin
R6577	T10372	T10371	punct	-,Smad2
R6578	T10373	T10369	pobj	skin,in
R6579	T10374	T10367	punct	", ",activated
R658	T1481	T1477	prep	into,growth
R6580	T10375	T10367	nsubjpass	Snail,activated
R6581	T10376	T10367	auxpass	is,activated
R6582	T10377	T10367	advmod	ectopically,activated
R6583	T10378	T10348	punct	.,demonstrate
R6584	T10380	T10381	advmod	Moreover,indicate
R6585	T10382	T10381	punct	", ",indicate
R6586	T10383	T10384	poss	our,studies
R6587	T10384	T10381	nsubj	studies,indicate
R6588	T10385	T10386	advmod	in,vitro
R6589	T10386	T10384	amod	vitro,studies
R659	T1482	T1483	det	the,dermis
R6590	T10387	T10388	mark	that,cause
R6591	T10388	T10381	ccomp	cause,indicate
R6592	T10389	T10390	advmod	even,exposure
R6593	T10390	T10388	nsubj	exposure,cause
R6594	T10391	T10390	amod	sustained,exposure
R6595	T10392	T10393	compound	TGF,β2
R6596	T10393	T10390	compound	β2,exposure
R6597	T10394	T10393	punct	-,β2
R6598	T10395	T10388	aux	may,cause
R6599	T10396	T10397	advmod	only,induction
R660	T1483	T1481	pobj	dermis,into
R6600	T10397	T10388	dobj	induction,cause
R6601	T10398	T10397	det	a,induction
R6602	T10399	T10397	amod	transient,induction
R6603	T10400	T10397	prep	of,induction
R6604	T10401	T10400	pobj	Snail,of
R6605	T10402	T10381	punct	", ",indicate
R6606	T10403	T10381	advcl	offering,indicate
R6607	T10404	T10405	det	a,explanation
R6608	T10405	T10403	dobj	explanation,offering
R6609	T10406	T10405	amod	possible,explanation
R661	T1484	T1483	amod	underlying,dermis
R6610	T10407	T10405	prep	as,explanation
R6611	T10408	T10407	prep	to,as
R6612	T10409	T10410	advmod	why,expressed
R6613	T10410	T10408	pcomp	expressed,to
R6614	T10411	T10410	nsubjpass	Snail,expressed
R6615	T10412	T10410	auxpass	is,expressed
R6616	T10413	T10414	advmod	so,briefly
R6617	T10414	T10410	advmod	briefly,expressed
R6618	T10415	T10410	prep	during,expressed
R6619	T10416	T10417	compound	hair,follicle
R662	T1485	T1477	punct	", ",growth
R6620	T10417	T10418	compound	follicle,morphogenesis
R6621	T10418	T10415	pobj	morphogenesis,during
R6622	T10419	T10381	punct	.,indicate
R6623	T10421	T10422	det	An,point
R6624	T10422	T10424	nsubj	point,is
R6625	T10423	T10422	amod	additional,point
R6626	T10425	T10422	amod	worth,point
R6627	T10426	T10425	advcl	mentioning,worth
R6628	T10427	T10428	mark	that,resulted
R6629	T10428	T10424	ccomp	resulted,is
R663	T1486	T1477	cc	or,growth
R6630	T10429	T10430	amod	prolonged,expression
R6631	T10430	T10428	nsubj	expression,resulted
R6632	T10431	T10430	prep	of,expression
R6633	T10432	T10433	compound	Tg,Snail
R6634	T10433	T10431	pobj	Snail,of
R6635	T10434	T10430	prep	in,expression
R6636	T10435	T10436	amod	postnatal,skin
R6637	T10436	T10434	pobj	skin,in
R6638	T10437	T10428	prep	in,resulted
R6639	T10438	T10439	amod	morphological,signs
R664	T1487	T1488	amod	down,growth
R6640	T10439	T10437	pobj	signs,in
R6641	T10440	T10438	cc	and,morphological
R6642	T10441	T10438	conj	biochemical,morphological
R6643	T10442	T10439	prep	of,signs
R6644	T10443	T10444	amod	epithelial,transitions
R6645	T10444	T10442	pobj	transitions,of
R6646	T10445	T10443	prep	to,epithelial
R6647	T10446	T10445	amod	mesenchymal,to
R6648	T10447	T10448	punct	(,data
R6649	T10448	T10428	meta	data,resulted
R665	T1488	T1477	conj	growth,growth
R6650	T10449	T10448	amod	unpublished,data
R6651	T10450	T10448	punct	),data
R6652	T10451	T10428	punct	", ",resulted
R6653	T10452	T10428	advcl	underscoring,resulted
R6654	T10453	T10454	advmod	why,be
R6655	T10454	T10452	ccomp	be,underscoring
R6656	T10455	T10456	amod	transient,expression
R6657	T10456	T10454	nsubj	expression,be
R6658	T10457	T10456	compound	Snail,expression
R6659	T10458	T10454	aux	may,be
R666	T1489	T1488	punct	-,growth
R6660	T10459	T10460	advmod	so,important
R6661	T10460	T10454	acomp	important,be
R6662	T10461	T10454	prep	during,be
R6663	T10462	T10463	amod	normal,morphogenesis
R6664	T10463	T10461	pobj	morphogenesis,during
R6665	T10464	T10465	compound	hair,follicle
R6666	T10465	T10463	compound	follicle,morphogenesis
R6667	T10466	T10467	punct	[,18
R6668	T10467	T10424	parataxis	18,is
R6669	T10468	T10467	punct	],18
R667	T1490	T1488	punct	", ",growth
R6670	T10469	T10424	punct	.,is
R6671	T10471	T10472	prep	At,seemed
R6672	T10473	T10474	amod	first,glance
R6673	T10474	T10471	pobj	glance,At
R6674	T10475	T10472	punct	", ",seemed
R6675	T10476	T10477	det	the,sparsity
R6676	T10477	T10472	nsubj	sparsity,seemed
R6677	T10478	T10477	prep	in,sparsity
R6678	T10479	T10480	compound	hair,coat
R6679	T10480	T10478	pobj	coat,in
R668	T1491	T1488	cc	and,growth
R6680	T10481	T10480	prep	of,coat
R6681	T10482	T10483	compound	K14,Snail
R6682	T10483	T10485	compound	Snail,mice
R6683	T10484	T10483	punct	-,Snail
R6684	T10485	T10481	pobj	mice,of
R6685	T10486	T10485	compound	Tg,mice
R6686	T10487	T10472	oprd	indicative,seemed
R6687	T10488	T10487	prep	of,indicative
R6688	T10489	T10490	det	a,defect
R6689	T10490	T10488	pobj	defect,of
R669	T1492	T1493	amod	eventual,differentiation
R6690	T10491	T10490	prep	in,defect
R6691	T10492	T10493	compound	follicle,formation
R6692	T10493	T10491	pobj	formation,in
R6693	T10494	T10495	punct	(,see
R6694	T10495	T10472	parataxis	see,seemed
R6695	T10496	T10497	compound	Figure,2A
R6696	T10497	T10495	dobj	2A,see
R6697	T10498	T10495	punct	),see
R6698	T10499	T10472	punct	.,seemed
R6699	T10501	T10502	amod	Closer,inspection
R670	T1493	T1488	conj	differentiation,growth
R6700	T10502	T10503	nsubj	inspection,revealed
R6701	T10504	T10503	punct	", ",revealed
R6702	T10505	T10503	advmod	however,revealed
R6703	T10506	T10503	punct	", ",revealed
R6704	T10507	T10508	mark	that,penetrated
R6705	T10508	T10503	ccomp	penetrated,revealed
R6706	T10509	T10510	neg	not,hairs
R6707	T10510	T10508	nsubj	hairs,penetrated
R6708	T10511	T10510	det	all,hairs
R6709	T10512	T10513	det	the,epidermis
R671	T1494	T1493	prep	into,differentiation
R6710	T10513	T10508	dobj	epidermis,penetrated
R6711	T10514	T10513	amod	hyperthickened,epidermis
R6712	T10515	T10513	compound	Tg,epidermis
R6713	T10516	T10503	punct	.,revealed
R6714	T10518	T10519	amod	Several,factors
R6715	T10519	T10520	nsubj	factors,contribute
R6716	T10521	T10520	aux	may,contribute
R6717	T10522	T10520	prep	to,contribute
R6718	T10523	T10524	det	the,development
R6719	T10524	T10522	pobj	development,to
R672	T1495	T1496	det	the,layers
R6720	T10525	T10526	advmod	seemingly,normal
R6721	T10526	T10524	amod	normal,development
R6722	T10527	T10524	compound	follicle,development
R6723	T10528	T10524	prep	in,development
R6724	T10529	T10530	det	these,mice
R6725	T10530	T10528	pobj	mice,in
R6726	T10531	T10520	punct	.,contribute
R6727	T10533	T10534	nummod	One,factor
R6728	T10534	T10536	nsubj	factor,is
R6729	T10535	T10534	amod	obvious,factor
R673	T1496	T1494	pobj	layers,into
R6730	T10537	T10538	det	the,promoter
R6731	T10538	T10536	attr	promoter,is
R6732	T10539	T10538	compound	K14,promoter
R6733	T10540	T10538	punct	", ",promoter
R6734	T10541	T10542	dep	which,is
R6735	T10542	T10538	relcl	is,promoter
R6736	T10543	T10542	acomp	elevated,is
R6737	T10544	T10542	prep	in,is
R6738	T10545	T10546	det	the,layer
R6739	T10546	T10544	pobj	layer,in
R674	T1497	T1496	nummod	six,layers
R6740	T10547	T10546	amod	basal,layer
R6741	T10548	T10546	prep	of,layer
R6742	T10549	T10550	det	the,epidermis
R6743	T10550	T10548	pobj	epidermis,of
R6744	T10551	T10546	cc	and,layer
R6745	T10552	T10553	det	the,sheath
R6746	T10553	T10546	conj	sheath,layer
R6747	T10554	T10553	amod	outer,sheath
R6748	T10555	T10553	compound	root,sheath
R6749	T10556	T10553	punct	(,sheath
R675	T1498	T1496	amod	concentric,layers
R6750	T10557	T10553	appos	ORS,sheath
R6751	T10558	T10553	punct	),sheath
R6752	T10559	T10553	prep	of,sheath
R6753	T10560	T10561	det	the,follicle
R6754	T10561	T10559	pobj	follicle,of
R6755	T10562	T10561	compound	hair,follicle
R6756	T10563	T10536	punct	", ",is
R6757	T10564	T10536	cc	but,is
R6758	T10565	T10566	auxpass	is,regulated
R6759	T10566	T10536	conj	regulated,is
R676	T1499	T1496	prep	of,layers
R6760	T10567	T10566	advmod	markedly,regulated
R6761	T10568	T10566	advmod	down,regulated
R6762	T10569	T10566	punct	-,regulated
R6763	T10570	T10566	prep	in,regulated
R6764	T10571	T10572	amod	developing,buds
R6765	T10572	T10570	pobj	buds,in
R6766	T10573	T10572	amod	embryonic,buds
R6767	T10574	T10572	compound	hair,buds
R6768	T10575	T10576	advmod	as,as
R6769	T10576	T10570	cc	as,in
R677	T1500	T1501	det	the,follicle
R6770	T10577	T10576	advmod	well,as
R6771	T10578	T10570	conj	in,in
R6772	T10579	T10580	det	the,cells
R6773	T10580	T10578	pobj	cells,in
R6774	T10581	T10580	amod	postnatal,cells
R6775	T10582	T10580	compound	hair,cells
R6776	T10583	T10580	compound	progenitor,cells
R6777	T10584	T10536	punct	.,is
R6778	T10586	T10587	det	The,promoter
R6779	T10587	T10589	nsubj	promoter,is
R678	T1501	T1499	pobj	follicle,of
R6780	T10588	T10587	compound	K14,promoter
R6781	T10590	T10589	advmod	also,is
R6782	T10591	T10592	advmod	less,active
R6783	T10592	T10589	acomp	active,is
R6784	T10593	T10592	prep	in,active
R6785	T10594	T10595	det	the,ORS
R6786	T10595	T10593	pobj	ORS,in
R6787	T10596	T10592	prep	than,active
R6788	T10597	T10596	pcomp	epidermis,than
R6789	T10598	T10589	cc	and,is
R679	T1502	T1501	amod	mature,follicle
R6790	T10599	T10600	advmod	hence,account
R6791	T10600	T10589	conj	account,is
R6792	T10601	T10600	nsubj	this,account
R6793	T10602	T10600	aux	might,account
R6794	T10603	T10600	advmod	also,account
R6795	T10604	T10600	prep	for,account
R6796	T10605	T10606	det	the,lack
R6797	T10606	T10604	pobj	lack,for
R6798	T10607	T10606	prep	of,lack
R6799	T10608	T10609	amod	apparent,response
R680	T1503	T1467	punct	.,result
R6800	T10609	T10607	pobj	response,of
R6801	T10610	T10609	prep	of,response
R6802	T10611	T10612	det	the,ORS
R6803	T10612	T10610	pobj	ORS,of
R6804	T10613	T10609	prep	to,response
R6805	T10614	T10615	amod	ectopic,Snail
R6806	T10615	T10613	pobj	Snail,to
R6807	T10616	T10600	punct	.,account
R6808	T10618	T10619	amod	Additional,factors
R6809	T10619	T10621	nsubj	factors,be
R681	T1505	T1506	advmod	Previously,delineated
R6810	T10620	T10619	amod	contributing,factors
R6811	T10622	T10621	aux	could,be
R6812	T10623	T10624	det	the,multiplicity
R6813	T10624	T10621	attr	multiplicity,be
R6814	T10625	T10624	prep	of,multiplicity
R6815	T10626	T10627	compound	Snail,members
R6816	T10627	T10625	pobj	members,of
R6817	T10628	T10627	compound	family,members
R6818	T10629	T10627	cc	and,members
R6819	T10630	T10631	poss	their,expression
R682	T1507	T1506	punct	", ",delineated
R6820	T10631	T10627	conj	expression,members
R6821	T10632	T10631	amod	differential,expression
R6822	T10633	T10627	punct	", ",members
R6823	T10634	T10635	det	the,saturation
R6824	T10635	T10627	appos	saturation,members
R6825	T10636	T10635	punct	", ",saturation
R6826	T10637	T10635	cc	and,saturation
R6827	T10638	T10637	punct	/,and
R6828	T10639	T10637	cc	or,and
R6829	T10640	T10635	conj	diversity,saturation
R683	T1508	T1506	nsubj	we,delineated
R6830	T10641	T10635	prep	of,saturation
R6831	T10642	T10643	amod	regulatory,mechanisms
R6832	T10643	T10641	pobj	mechanisms,of
R6833	T10644	T10645	dep	that,govern
R6834	T10645	T10643	relcl	govern,mechanisms
R6835	T10646	T10647	compound	AJ,formation
R6836	T10647	T10645	dobj	formation,govern
R6837	T10648	T10647	punct	", ",formation
R6838	T10649	T10647	conj	migration,formation
R6839	T10650	T10649	punct	", ",migration
R684	T1509	T1510	advmod	how,function
R6840	T10651	T10649	cc	and,migration
R6841	T10652	T10649	conj	proliferation,migration
R6842	T10653	T10645	prep	in,govern
R6843	T10654	T10655	det	the,ORS
R6844	T10655	T10653	pobj	ORS,in
R6845	T10656	T10655	compound	follicle,ORS
R6846	T10657	T10621	punct	.,be
R6847	T10659	T10660	csubj	Distinguishing,await
R6848	T10661	T10659	prep	between,Distinguishing
R6849	T10662	T10663	det	these,possibilities
R685	T1510	T1506	ccomp	function,delineated
R6850	T10663	T10661	pobj	possibilities,between
R6851	T10664	T10660	aux	must,await
R6852	T10665	T10666	det	the,generation
R6853	T10666	T10660	dobj	generation,await
R6854	T10667	T10666	prep	of,generation
R6855	T10668	T10667	pobj	mice,of
R6856	T10669	T10668	acl	harboring,mice
R6857	T10670	T10671	npadvmod	skin,specific
R6858	T10671	T10673	amod	specific,mutations
R6859	T10672	T10671	punct	-,specific
R686	T1511	T1512	nummod	two,signals
R6860	T10673	T10669	dobj	mutations,harboring
R6861	T10674	T10673	nmod	loss,mutations
R6862	T10675	T10674	punct	-,loss
R6863	T10676	T10674	prep	of,loss
R6864	T10677	T10676	punct	-,of
R6865	T10678	T10676	pobj	function,of
R6866	T10679	T10673	compound	Snail,mutations
R6867	T10680	T10660	punct	.,await
R687	T1512	T1510	nsubj	signals,function
R6876	T11058	T11057	prep	between,Links
R6877	T11059	T11058	pobj	Signaling,between
R6878	T11060	T11059	punct	", ",Signaling
R6879	T11061	T11062	amod	Transcriptional,Cascades
R688	T1513	T1512	amod	respective,signals
R6880	T11062	T11059	conj	Cascades,Signaling
R6881	T11063	T11062	punct	", ",Cascades
R6882	T11064	T11065	amod	Epithelial,Remodeling
R6883	T11065	T11062	conj	Remodeling,Cascades
R6884	T11066	T11065	punct	", ",Remodeling
R6885	T11067	T11065	cc	and,Remodeling
R6886	T11068	T11065	conj	Proliferation,Remodeling
R6887	T11069	T11057	prep	in,Links
R6888	T11070	T11071	det	the,Bud
R6889	T11071	T11069	pobj	Bud,in
R689	T1514	T1512	amod	epithelial,signals
R6890	T11072	T11071	compound	Hair,Bud
R6891	T11074	T11075	advmod	Previously,discovered
R6892	T11076	T11075	punct	", ",discovered
R6893	T11077	T11075	nsubj	we,discovered
R6894	T11078	T11079	mark	that,regulated
R6895	T11079	T11075	ccomp	regulated,discovered
R6896	T11080	T11079	advmod	early,regulated
R6897	T11081	T11080	prep	during,early
R6898	T11082	T11083	compound	hair,follicle
R6899	T11083	T11084	compound	follicle,morphogenesis
R690	T1515	T1514	cc	and,epithelial
R6900	T11084	T11081	pobj	morphogenesis,during
R6901	T11085	T11079	punct	", ",regulated
R6902	T11086	T11087	compound	E,cadherin
R6903	T11087	T11089	compound	cadherin,expression
R6904	T11088	T11087	punct	-,cadherin
R6905	T11089	T11079	nsubjpass	expression,regulated
R6906	T11090	T11089	compound	gene,expression
R6907	T11091	T11079	auxpass	is,regulated
R6908	T11092	T11079	advmod	down,regulated
R6909	T11093	T11079	punct	-,regulated
R691	T1516	T1514	conj	mesenchymal,epithelial
R6910	T11094	T11079	advmod	concomitantly,regulated
R6911	T11095	T11079	prep	with,regulated
R6912	T11096	T11097	det	the,invagination
R6913	T11097	T11095	pobj	invagination,with
R6914	T11098	T11097	prep	of,invagination
R6915	T11099	T11100	amod	developing,cells
R6916	T11100	T11098	pobj	cells,of
R6917	T11101	T11100	compound	bud,cells
R6918	T11102	T11097	prep	into,invagination
R6919	T11103	T11104	det	the,skin
R692	T1517	T1512	punct	", ",signals
R6920	T11104	T11102	pobj	skin,into
R6921	T11105	T11106	punct	[,4
R6922	T11106	T11075	parataxis	4,discovered
R6923	T11107	T11106	punct	],4
R6924	T11108	T11075	punct	.,discovered
R6925	T11110	T11111	mark	Because,correlated
R6926	T11111	T11117	advcl	correlated,intrigued
R6927	T11112	T11113	det	the,timing
R6928	T11113	T11111	nsubj	timing,correlated
R6929	T11114	T11113	prep	of,timing
R693	T1518	T1512	appos	Wnts,signals
R6930	T11115	T11116	det	this,event
R6931	T11116	T11114	pobj	event,of
R6932	T11118	T11111	prep	with,correlated
R6933	T11119	T11120	det	the,activation
R6934	T11120	T11118	pobj	activation,with
R6935	T11121	T11120	prep	of,activation
R6936	T11122	T11123	det	a,complex
R6937	T11123	T11121	pobj	complex,of
R6938	T11124	T11125	nmod	LEF,factor
R6939	T11125	T11123	compound	factor,complex
R694	T1519	T1518	cc	and,Wnts
R6940	T11126	T11124	punct	-,LEF
R6941	T11127	T11124	nummod	1,LEF
R6942	T11128	T11124	punct	/,LEF
R6943	T11129	T11130	compound	β,catenin
R6944	T11130	T11124	appos	catenin,LEF
R6945	T11131	T11130	punct	-,catenin
R6946	T11132	T11125	compound	transcription,factor
R6947	T11133	T11134	punct	[,20
R6948	T11134	T11111	parataxis	20,correlated
R6949	T11135	T11134	punct	],20
R695	T1520	T1521	det	the,factor
R6950	T11136	T11117	punct	", ",intrigued
R6951	T11137	T11117	nsubjpass	we,intrigued
R6952	T11138	T11117	auxpass	were,intrigued
R6953	T11139	T11117	agent	by,intrigued
R6954	T11140	T11141	det	the,presence
R6955	T11141	T11139	pobj	presence,by
R6956	T11142	T11141	prep	of,presence
R6957	T11143	T11144	det	a,site
R6958	T11144	T11142	pobj	site,of
R6959	T11145	T11144	amod	putative,site
R696	T1521	T1518	conj	factor,Wnts
R6960	T11146	T11144	nmod	LEF,site
R6961	T11147	T11146	punct	-,LEF
R6962	T11148	T11146	nummod	1,LEF
R6963	T11149	T11146	punct	/,LEF
R6964	T11150	T11146	appos	TCF,LEF
R6965	T11151	T11144	compound	binding,site
R6966	T11152	T11141	prep	in,presence
R6967	T11153	T11154	det	the,promoter
R6968	T11154	T11152	pobj	promoter,in
R6969	T11155	T11156	compound	E,cadherin
R697	T1522	T1523	npadvmod	BMP,inhibitory
R6970	T11156	T11154	compound	cadherin,promoter
R6971	T11157	T11156	punct	-,cadherin
R6972	T11158	T11117	punct	.,intrigued
R6973	T11160	T11161	nsubj	This,prompted
R6974	T11162	T11163	det	an,investigation
R6975	T11163	T11161	dobj	investigation,prompted
R6976	T11164	T11165	dep	that,led
R6977	T11165	T11163	relcl	led,investigation
R6978	T11166	T11165	advmod	subsequently,led
R6979	T11167	T11165	prep	to,led
R698	T1523	T1521	amod	inhibitory,factor
R6980	T11168	T11169	poss	our,discovery
R6981	T11169	T11167	pobj	discovery,to
R6982	T11170	T11171	mark	that,contribute
R6983	T11171	T11169	acl	contribute,discovery
R6984	T11172	T11171	nsubj	LEF,contribute
R6985	T11173	T11172	punct	-,LEF
R6986	T11174	T11172	nummod	1,LEF
R6987	T11175	T11172	punct	/,LEF
R6988	T11176	T11177	compound	β,catenin
R6989	T11177	T11172	appos	catenin,LEF
R699	T1524	T1523	punct	-,inhibitory
R6990	T11178	T11177	punct	-,catenin
R6991	T11179	T11171	aux	can,contribute
R6992	T11180	T11171	prep	to,contribute
R6993	T11181	T11180	pobj	repression,to
R6994	T11182	T11181	prep	of,repression
R6995	T11183	T11184	compound	E,cadherin
R6996	T11184	T11186	compound	cadherin,expression
R6997	T11185	T11184	punct	-,cadherin
R6998	T11186	T11182	pobj	expression,of
R6999	T11187	T11186	compound	gene,expression
R700	T1525	T1521	appos	noggin,factor
R7000	T11188	T11171	prep	in,contribute
R7001	T11189	T11190	compound	skin,keratinocytes
R7002	T11190	T11188	pobj	keratinocytes,in
R7003	T11191	T11192	punct	[,4
R7004	T11192	T11161	parataxis	4,prompted
R7005	T11193	T11192	punct	],4
R7006	T11194	T11161	punct	.,prompted
R7007	T11196	T11197	prep	In,noted
R7008	T11198	T11199	det	the,course
R7009	T11199	T11196	pobj	course,In
R701	T1526	T1510	punct	", ",function
R7010	T11200	T11199	prep	of,course
R7011	T11201	T11202	det	these,studies
R7012	T11202	T11200	pobj	studies,of
R7013	T11203	T11197	punct	", ",noted
R7014	T11204	T11197	nsubj	we,noted
R7015	T11205	T11197	advmod	also,noted
R7016	T11206	T11207	mark	that,contribute
R7017	T11207	T11197	ccomp	contribute,noted
R7018	T11208	T11207	nsubj	Snail,contribute
R7019	T11209	T11207	aux	can,contribute
R702	T1527	T1510	prep	in,function
R7020	T11210	T11207	advmod	also,contribute
R7021	T11211	T11207	prep	to,contribute
R7022	T11212	T11213	det	this,process
R7023	T11213	T11211	pobj	process,to
R7024	T11214	T11207	prep	in,contribute
R7025	T11215	T11214	pobj	keratinocytes,in
R7026	T11216	T11217	advmod	in,vitro
R7027	T11217	T11207	advmod	vitro,contribute
R7028	T11218	T11197	punct	", ",noted
R7029	T11219	T11197	cc	and,noted
R703	T1528	T1527	pobj	concert,in
R7030	T11220	T11221	poss	our,studies
R7031	T11221	T11223	nsubj	studies,revealed
R7032	T11222	T11221	amod	present,studies
R7033	T11223	T11197	conj	revealed,noted
R7034	T11224	T11225	mark	that,expressed
R7035	T11225	T11223	ccomp	expressed,revealed
R7036	T11226	T11225	nsubjpass	Snail,expressed
R7037	T11227	T11225	auxpass	is,expressed
R7038	T11228	T11225	prep	at,expressed
R7039	T11229	T11230	det	the,place
R704	T1529	T1530	aux	to,induce
R7040	T11230	T11228	pobj	place,at
R7041	T11231	T11230	amod	right,place
R7042	T11232	T11230	cc	and,place
R7043	T11233	T11230	conj	time,place
R7044	T11234	T11235	aux	to,be
R7045	T11235	T11230	advcl	be,place
R7046	T11236	T11237	advmod	physiologically,relevant
R7047	T11237	T11235	acomp	relevant,be
R7048	T11238	T11235	prep	in,be
R7049	T11239	T11240	det	the,process
R705	T1530	T1510	advcl	induce,function
R7050	T11240	T11238	pobj	process,in
R7051	T11241	T11223	punct	.,revealed
R7052	T11243	T11244	prep	In,abrogated
R7053	T11245	T11246	npadvmod	noggin,null
R7054	T11246	T11248	amod	null,skin
R7055	T11247	T11246	punct	-,null
R7056	T11248	T11243	pobj	skin,In
R7057	T11249	T11248	amod	embryonic,skin
R7058	T11250	T11244	punct	", ",abrogated
R7059	T11251	T11252	nmod	LEF,expression
R706	T1531	T1532	amod	lymphoid,factor
R7060	T11252	T11244	nsubjpass	expression,abrogated
R7061	T11253	T11251	punct	-,LEF
R7062	T11254	T11251	nummod	1,LEF
R7063	T11255	T11252	cc	and,expression
R7064	T11256	T11257	amod	subsequent,activation
R7065	T11257	T11252	conj	activation,expression
R7066	T11258	T11257	prep	of,activation
R7067	T11259	T11260	det	the,gene
R7068	T11260	T11258	pobj	gene,of
R7069	T11261	T11260	nmod	LEF,gene
R707	T1532	T1534	npadvmod	factor,mediated
R7070	T11262	T11261	punct	-,LEF
R7071	T11263	T11261	nummod	1,LEF
R7072	T11264	T11261	punct	/,LEF
R7073	T11265	T11266	compound	β,catenin
R7074	T11266	T11261	appos	catenin,LEF
R7075	T11267	T11266	punct	-,catenin
R7076	T11268	T11260	compound	reporter,gene
R7077	T11269	T11244	auxpass	is,abrogated
R7078	T11270	T11244	prep	in,abrogated
R7079	T11271	T11272	det	the,placodes
R708	T1533	T1532	compound	enhancer,factor
R7080	T11272	T11270	pobj	placodes,in
R7081	T11273	T11272	amod	developing,placodes
R7082	T11274	T11244	punct	.,abrogated
R7083	T11276	T11277	det	The,failure
R7084	T11277	T11279	nsubj	failure,underscores
R7085	T11278	T11277	amod	corresponding,failure
R7086	T11280	T11277	prep	of,failure
R7087	T11281	T11282	nmod	E,cadherin
R7088	T11282	T11284	nmod	cadherin,regulation
R7089	T11283	T11282	punct	-,cadherin
R709	T1534	T1551	amod	mediated,transcription
R7090	T11284	T11280	pobj	regulation,of
R7091	T11285	T11284	amod	down,regulation
R7092	T11286	T11284	punct	-,regulation
R7093	T11287	T11288	det	the,importance
R7094	T11288	T11279	dobj	importance,underscores
R7095	T11289	T11288	prep	of,importance
R7096	T11290	T11291	compound	Wnt,noggin
R7097	T11291	T11293	compound	noggin,signaling
R7098	T11292	T11291	punct	/,noggin
R7099	T11293	T11289	pobj	signaling,of
R710	T1535	T1532	punct	-,factor
R7100	T11294	T11288	prep	in,importance
R7101	T11295	T11294	pcomp	regulating,in
R7102	T11296	T11297	det	this,event
R7103	T11297	T11295	dobj	event,regulating
R7104	T11298	T11295	prep	in,regulating
R7105	T11299	T11300	compound	follicle,morphogenesis
R7106	T11300	T11298	pobj	morphogenesis,in
R7107	T11301	T11302	punct	[,4
R7108	T11302	T11279	parataxis	4,underscores
R7109	T11303	T11302	punct	],4
R711	T1536	T1532	nummod	1,factor
R7110	T11304	T11279	punct	.,underscores
R7111	T11306	T11307	amod	Conditional,studies
R7112	T11307	T11310	nsubj	studies,be
R7113	T11308	T11309	compound	gene,targeting
R7114	T11309	T11307	compound	targeting,studies
R7115	T11311	T11310	aux	will,be
R7116	T11312	T11310	acomp	necessary,be
R7117	T11313	T11314	aux	to,establish
R7118	T11314	T11310	advcl	establish,be
R7119	T11315	T11316	mark	whether,contribute
R712	T1537	T1532	punct	/,factor
R7120	T11316	T11314	ccomp	contribute,establish
R7121	T11317	T11318	compound	Snail,members
R7122	T11318	T11316	nsubj	members,contribute
R7123	T11319	T11318	compound	family,members
R7124	T11320	T11316	advmod	also,contribute
R7125	T11321	T11316	prep	to,contribute
R7126	T11322	T11323	det	the,regulation
R7127	T11323	T11321	pobj	regulation,to
R7128	T11324	T11323	amod	down,regulation
R7129	T11325	T11323	punct	-,regulation
R713	T1538	T1539	compound	β,catenin
R7130	T11326	T11323	prep	in,regulation
R7131	T11327	T11328	compound	E,cadherin
R7132	T11328	T11330	compound	cadherin,expression
R7133	T11329	T11328	punct	-,cadherin
R7134	T11330	T11326	pobj	expression,in
R7135	T11331	T11330	compound	gene,expression
R7136	T11332	T11333	dep	that,occurs
R7137	T11333	T11323	relcl	occurs,regulation
R7138	T11334	T11333	prep	during,occurs
R7139	T11335	T11336	compound	follicle,formation
R714	T1539	T1532	appos	catenin,factor
R7140	T11336	T11334	pobj	formation,during
R7141	T11337	T11310	punct	.,be
R7142	T11339	T11340	advmod	However,is
R7143	T11341	T11340	punct	", ",is
R7144	T11342	T11340	nsubj	it,is
R7145	T11343	T11340	acomp	intriguing,is
R7146	T11344	T11345	mark	that,displayed
R7147	T11345	T11340	ccomp	displayed,is
R7148	T11346	T11347	compound	K14,Snail
R7149	T11347	T11349	compound	Snail,epidermis
R715	T1540	T1539	punct	-,catenin
R7150	T11348	T11347	punct	-,Snail
R7151	T11349	T11345	nsubj	epidermis,displayed
R7152	T11350	T11349	compound	Tg,epidermis
R7153	T11351	T11352	det	a,regulation
R7154	T11352	T11345	dobj	regulation,displayed
R7155	T11353	T11352	amod	marked,regulation
R7156	T11354	T11352	amod	down,regulation
R7157	T11355	T11352	punct	-,regulation
R7158	T11356	T11352	prep	in,regulation
R7159	T11357	T11358	compound	E,cadherin
R716	T1541	T1532	punct	(,factor
R7160	T11358	T11360	compound	cadherin,expression
R7161	T11359	T11358	punct	-,cadherin
R7162	T11360	T11356	pobj	expression,in
R7163	T11361	T11345	punct	", ",displayed
R7164	T11362	T11363	advmod	thereby,demonstrating
R7165	T11363	T11345	advcl	demonstrating,displayed
R7166	T11364	T11365	poss	its,potential
R7167	T11365	T11363	dobj	potential,demonstrating
R7168	T11366	T11367	aux	to,do
R7169	T11367	T11365	acl	do,potential
R717	T1542	T1532	appos	LEF,factor
R7170	T11368	T11367	advmod	so,do
R7171	T11369	T11367	prep	in,do
R7172	T11370	T11369	pobj	skin,in
R7173	T11371	T11340	punct	.,is
R7174	T11373	T11374	poss	Our,findings
R7175	T11374	T11376	nsubj	findings,showed
R7176	T11375	T11374	amod	prior,findings
R7177	T11377	T11378	mark	that,impaired
R7178	T11378	T11376	ccomp	impaired,showed
R7179	T11379	T11378	prep	by,impaired
R718	T1543	T1542	punct	-,LEF
R7180	T11380	T11379	pcomp	elevating,by
R7181	T11381	T11382	compound	E,cadherin
R7182	T11382	T11384	compound	cadherin,levels
R7183	T11383	T11382	punct	-,cadherin
R7184	T11384	T11380	dobj	levels,elevating
R7185	T11385	T11379	cc	or,by
R7186	T11386	T11379	conj	by,by
R7187	T11387	T11388	advmod	conditionally,ablating
R7188	T11388	T11386	pcomp	ablating,by
R7189	T11389	T11390	compound	α,catenin
R719	T1544	T1542	nummod	1,LEF
R7190	T11390	T11388	dobj	catenin,ablating
R7191	T11391	T11390	punct	-,catenin
R7192	T11392	T11378	punct	", ",impaired
R7193	T11393	T11394	compound	hair,follicle
R7194	T11394	T11395	compound	follicle,morphogenesis
R7195	T11395	T11378	nsubjpass	morphogenesis,impaired
R7196	T11396	T11378	aux	can,impaired
R7197	T11397	T11378	auxpass	be,impaired
R7198	T11398	T11399	punct	[,7
R7199	T11399	T11376	parataxis	7,showed
R720	T1545	T1542	punct	/,LEF
R7200	T11400	T11399	nummod	4,7
R7201	T11401	T11399	punct	",",7
R7202	T11402	T11399	punct	],7
R7203	T11403	T11376	punct	.,showed
R7204	T11405	T11406	det	The,hyperproliferation
R7205	T11406	T11409	nsubj	hyperproliferation,led
R7206	T11407	T11406	amod	marked,hyperproliferation
R7207	T11408	T11406	amod	epidermal,hyperproliferation
R7208	T11410	T11406	acl	seen,hyperproliferation
R7209	T11411	T11410	prep	in,seen
R721	T1546	T1547	compound	β,catenin
R7210	T11412	T11413	det	the,skin
R7211	T11413	T11411	pobj	skin,in
R7212	T11414	T11415	compound	K14,Snail
R7213	T11415	T11413	compound	Snail,skin
R7214	T11416	T11415	punct	-,Snail
R7215	T11417	T11413	compound	Tg,skin
R7216	T11418	T11406	punct	", ",hyperproliferation
R7217	T11419	T11406	acl	coupled,hyperproliferation
R7218	T11420	T11419	prep	with,coupled
R7219	T11421	T11422	det	the,suppression
R722	T1547	T1542	appos	catenin,LEF
R7220	T11422	T11420	pobj	suppression,with
R7221	T11423	T11422	amod	converse,suppression
R7222	T11424	T11422	prep	of,suppression
R7223	T11425	T11424	pobj	proliferation,of
R7224	T11426	T11425	cc	and,proliferation
R7225	T11427	T11425	conj	Snail,proliferation
R7226	T11428	T11422	prep	in,suppression
R7227	T11429	T11430	compound	TGF,β2
R7228	T11430	T11432	npadvmod	β2,null
R7229	T11431	T11430	punct	-,β2
R723	T1548	T1547	punct	-,catenin
R7230	T11432	T11434	amod	null,buds
R7231	T11433	T11432	punct	-,null
R7232	T11434	T11428	pobj	buds,in
R7233	T11435	T11434	compound	hair,buds
R7234	T11436	T11409	dobj	us,led
R7235	T11437	T11438	aux	to,wonder
R7236	T11438	T11409	xcomp	wonder,led
R7237	T11439	T11440	mark	whether,have
R7238	T11440	T11438	ccomp	have,wonder
R7239	T11441	T11442	det	the,regulation
R724	T1549	T1534	punct	),mediated
R7240	T11442	T11440	nsubj	regulation,have
R7241	T11443	T11442	amod	down,regulation
R7242	T11444	T11442	punct	-,regulation
R7243	T11445	T11442	prep	of,regulation
R7244	T11446	T11447	compound	E,cadherin
R7245	T11447	T11445	pobj	cadherin,of
R7246	T11448	T11447	punct	-,cadherin
R7247	T11449	T11442	prep	during,regulation
R7248	T11450	T11451	compound	follicle,morphogenesis
R7249	T11451	T11449	pobj	morphogenesis,during
R725	T1550	T1534	punct	-,mediated
R7250	T11452	T11440	aux	might,have
R7251	T11453	T11454	det	a,impact
R7252	T11454	T11440	dobj	impact,have
R7253	T11455	T11454	amod	direct,impact
R7254	T11456	T11454	prep	on,impact
R7255	T11457	T11456	pcomp	elevating,on
R7256	T11458	T11459	det	the,state
R7257	T11459	T11457	dobj	state,elevating
R7258	T11460	T11459	amod	proliferative,state
R7259	T11461	T11459	prep	of,state
R726	T1551	T1530	dobj	transcription,induce
R7260	T11462	T11463	det	these,cells
R7261	T11463	T11461	pobj	cells,of
R7262	T11464	T11409	punct	.,led
R7263	T11466	T11467	poss	Our,studies
R7264	T11467	T11469	nsubj	studies,suggested
R7265	T11468	T11467	compound	Tg,studies
R7266	T11470	T11471	mark	that,is
R7267	T11471	T11469	ccomp	is,suggested
R7268	T11472	T11471	punct	", ",is
R7269	T11473	T11474	advmod	at,least
R727	T1552	T1551	compound	gene,transcription
R7270	T11474	T11475	advmod	least,in
R7271	T11475	T11476	prep	in,through
R7272	T11476	T11471	prep	through,is
R7273	T11477	T11475	pobj	part,in
R7274	T11478	T11479	poss	its,regulation
R7275	T11479	T11476	pobj	regulation,through
R7276	T11480	T11479	prep	of,regulation
R7277	T11481	T11482	compound	E,cadherin
R7278	T11482	T11480	pobj	cadherin,of
R7279	T11483	T11482	punct	-,cadherin
R728	T1553	T1530	prep	within,induce
R7280	T11484	T11471	punct	", ",is
R7281	T11485	T11471	nsubj	Snail,is
R7282	T11486	T11471	acomp	able,is
R7283	T11487	T11488	aux	to,influence
R7284	T11488	T11486	xcomp	influence,able
R7285	T11489	T11490	det	the,localization
R7286	T11490	T11488	dobj	localization,influence
R7287	T11491	T11490	amod	subcellular,localization
R7288	T11492	T11490	prep	of,localization
R7289	T11493	T11494	det	a,variety
R729	T1554	T1555	det	the,placode
R7290	T11494	T11492	pobj	variety,of
R7291	T11495	T11494	prep	of,variety
R7292	T11496	T11497	npadvmod	AJ,associated
R7293	T11497	T11499	amod	associated,proteins
R7294	T11498	T11497	punct	-,associated
R7295	T11499	T11495	pobj	proteins,of
R7296	T11500	T11469	punct	.,suggested
R7297	T11502	T11503	nsubj	One,appears
R7298	T11504	T11502	prep	of,One
R7299	T11505	T11504	pobj	these,of
R730	T1555	T1553	pobj	placode,within
R7300	T11506	T11507	aux	to,be
R7301	T11507	T11503	xcomp	be,appears
R7302	T11508	T11507	attr	Ajuba,be
R7303	T11509	T11508	punct	", ",Ajuba
R7304	T11510	T11511	dep	which,shown
R7305	T11511	T11508	relcl	shown,Ajuba
R7306	T11512	T11511	auxpass	was,shown
R7307	T11513	T11511	advmod	previously,shown
R7308	T11514	T11515	aux	to,have
R7309	T11515	T11511	xcomp	have,shown
R731	T1556	T1555	compound	follicle,placode
R7310	T11516	T11517	det	the,capacity
R7311	T11517	T11515	dobj	capacity,have
R7312	T11518	T11517	amod	dual,capacity
R7313	T11519	T11520	aux	to,bind
R7314	T11520	T11517	acl	bind,capacity
R7315	T11521	T11520	dobj	Grb,bind
R7316	T11522	T11521	punct	-,Grb
R7317	T11523	T11521	nummod	2,Grb
R7318	T11524	T11525	advmod	as,as
R7319	T11525	T11521	cc	as,Grb
R732	T1557	T1558	punct	[,4
R7320	T11526	T11525	advmod	well,as
R7321	T11527	T11528	compound	α,catenin
R7322	T11528	T11521	conj	catenin,Grb
R7323	T11529	T11528	punct	-,catenin
R7324	T11530	T11531	punct	[,10
R7325	T11531	T11503	parataxis	10,appears
R7326	T11532	T11531	nummod	9,10
R7327	T11533	T11531	punct	",",10
R7328	T11534	T11531	punct	],10
R7329	T11535	T11503	punct	.,appears
R733	T1558	T1506	parataxis	4,delineated
R7330	T11537	T11538	poss	Our,studies
R7331	T11538	T11539	nsubj	studies,revealed
R7332	T11540	T11541	mark	that,develops
R7333	T11541	T11539	ccomp	develops,revealed
R7334	T11542	T11541	prep	in,develops
R7335	T11543	T11544	compound	skin,keratinocytes
R7336	T11544	T11542	pobj	keratinocytes,in
R7337	T11545	T11546	dep	that,harbor
R7338	T11546	T11544	advcl	harbor,keratinocytes
R7339	T11547	T11546	preconj	either,harbor
R734	T1559	T1558	punct	],4
R7340	T11548	T11549	det	a,mutation
R7341	T11549	T11546	dobj	mutation,harbor
R7342	T11550	T11549	amod	conditional,mutation
R7343	T11551	T11549	amod	null,mutation
R7344	T11552	T11546	prep	in,harbor
R7345	T11553	T11554	compound	α,catenin
R7346	T11554	T11552	pobj	catenin,in
R7347	T11555	T11554	punct	-,catenin
R7348	T11556	T11546	cc	or,harbor
R7349	T11557	T11558	dep	that,overexpress
R735	T1560	T1506	punct	.,delineated
R7350	T11558	T11546	conj	overexpress,harbor
R7351	T11559	T11558	dobj	Snail,overexpress
R7352	T11560	T11541	punct	", ",develops
R7353	T11561	T11541	nsubj	Ajuba,develops
R7354	T11562	T11563	det	an,interaction
R7355	T11563	T11541	dobj	interaction,develops
R7356	T11564	T11563	prep	with,interaction
R7357	T11565	T11564	pobj	Grb,with
R7358	T11566	T11565	punct	-,Grb
R7359	T11567	T11565	nummod	2,Grb
R736	T1562	T1563	det	The,changes
R7360	T11568	T11569	dep	that,observed
R7361	T11569	T11563	relcl	observed,interaction
R7362	T11570	T11569	auxpass	is,observed
R7363	T11571	T11569	advmod	otherwise,observed
R7364	T11572	T11569	neg	not,observed
R7365	T11573	T11569	prep	in,observed
R7366	T11574	T11575	compound	WT,keratinocytes
R7367	T11575	T11573	pobj	keratinocytes,in
R7368	T11576	T11539	punct	.,revealed
R7369	T11578	T11579	det	The,abilities
R737	T1563	T1565	nsubj	changes,include
R7370	T11579	T11581	nsubj	abilities,suggest
R7371	T11580	T11579	amod	corresponding,abilities
R7372	T11582	T11579	prep	of,abilities
R7373	T11583	T11584	preconj	either,keratinocytes
R7374	T11584	T11582	pobj	keratinocytes,of
R7375	T11585	T11586	npadvmod	Snail,transfected
R7376	T11586	T11584	amod	transfected,keratinocytes
R7377	T11587	T11586	punct	-,transfected
R7378	T11588	T11586	cc	or,transfected
R7379	T11589	T11590	npadvmod	Ajuba,transfected
R738	T1564	T1563	amod	downstream,changes
R7380	T11590	T11586	conj	transfected,transfected
R7381	T11591	T11590	punct	-,transfected
R7382	T11592	T11593	aux	to,exhibit
R7383	T11593	T11579	acl	exhibit,abilities
R7384	T11594	T11595	amod	elevated,activation
R7385	T11595	T11593	dobj	activation,exhibit
R7386	T11596	T11595	prep	of,activation
R7387	T11597	T11598	det	the,pathway
R7388	T11598	T11596	pobj	pathway,of
R7389	T11599	T11600	compound	Ras,MAPK
R739	T1566	T1563	acl	elicited,changes
R7390	T11600	T11598	compound	MAPK,pathway
R7391	T11601	T11600	punct	-,MAPK
R7392	T11602	T11603	mark	that,be
R7393	T11603	T11581	ccomp	be,suggest
R7394	T11604	T11605	det	the,association
R7395	T11605	T11603	nsubj	association,be
R7396	T11606	T11605	nmod	Grb,association
R7397	T11607	T11606	punct	-,Grb
R7398	T11608	T11606	nummod	2,Grb
R7399	T11609	T11605	prep	of,association
R740	T1567	T1566	prep	through,elicited
R7400	T11610	T11609	pobj	Ajuba,of
R7401	T11611	T11603	prep	under,be
R7402	T11612	T11611	pobj	conditions,under
R7403	T11613	T11612	prep	of,conditions
R7404	T11614	T11615	amod	reduced,levels
R7405	T11615	T11613	pobj	levels,of
R7406	T11616	T11615	prep	of,levels
R7407	T11617	T11618	compound	AJ,proteins
R7408	T11618	T11616	pobj	proteins,of
R7409	T11619	T11603	aux	may,be
R741	T1568	T1567	pobj	convergence,through
R7410	T11620	T11621	advmod	directly,relevant
R7411	T11621	T11603	acomp	relevant,be
R7412	T11622	T11621	prep	to,relevant
R7413	T11623	T11624	det	the,parallel
R7414	T11624	T11622	pobj	parallel,to
R7415	T11625	T11624	prep	in,parallel
R7416	T11626	T11625	pobj	hyperproliferation,in
R7417	T11627	T11581	punct	.,suggest
R7418	T11629	T11630	prep	In,shown
R7419	T11631	T11632	amod	stable,lines
R742	T1569	T1568	prep	of,convergence
R7420	T11632	T11629	pobj	lines,In
R7421	T11633	T11632	amod	epithelial,lines
R7422	T11634	T11635	punct	(,kidney
R7423	T11635	T11632	parataxis	kidney,lines
R7424	T11636	T11635	advmod	i.e.,kidney
R7425	T11637	T11635	punct	", ",kidney
R7426	T11638	T11639	nmod	Madin,Darby
R7427	T11639	T11635	nmod	Darby,kidney
R7428	T11640	T11639	punct	-,Darby
R7429	T11641	T11635	amod	canine,kidney
R743	T1675	T1677	compound	cell,contact
R7430	T11642	T11635	punct	", ",kidney
R7431	T11643	T11635	cc	or,kidney
R7432	T11644	T11635	conj	MDCK,kidney
R7433	T11645	T11635	punct	),kidney
R7434	T11646	T11632	compound	cell,lines
R7435	T11647	T11630	punct	", ",shown
R7436	T11648	T11630	nsubjpass	Snail,shown
R7437	T11649	T11630	aux	has,shown
R7438	T11650	T11630	auxpass	been,shown
R7439	T11651	T11652	aux	to,block
R744	T1676	T1675	punct	-,cell
R7440	T11652	T11630	xcomp	block,shown
R7441	T11653	T11654	compound	cell,cycle
R7442	T11654	T11655	compound	cycle,progression
R7443	T11655	T11652	dobj	progression,block
R7444	T11656	T11652	cc	and,block
R7445	T11657	T11652	conj	promote,block
R7446	T11658	T11659	nmod	motility,changes
R7447	T11659	T11657	dobj	changes,promote
R7448	T11660	T11658	cc	and,motility
R7449	T11661	T11658	conj	shape,motility
R745	T1677	T1673	pobj	contact,of
R7450	T11662	T11657	prep	for,promote
R7451	T11663	T11662	pobj	invasion,for
R7452	T11664	T11665	punct	[,47
R7453	T11665	T11630	parataxis	47,shown
R7454	T11666	T11665	punct	],47
R7455	T11667	T11630	punct	.,shown
R7456	T11669	T11670	mark	While,are
R7457	T11670	T11675	advcl	are,differ
R7458	T11671	T11672	poss	our,studies
R7459	T11672	T11670	nsubj	studies,are
R746	T1678	T1671	punct	", ",recruit
R7460	T11673	T11674	advmod	in,vivo
R7461	T11674	T11672	amod	vivo,studies
R7462	T11676	T11670	acomp	consistent,are
R7463	T11677	T11676	prep	with,consistent
R7464	T11678	T11679	det	a,role
R7465	T11679	T11677	pobj	role,with
R7466	T11680	T11679	prep	for,role
R7467	T11681	T11680	pobj	Snail,for
R7468	T11682	T11679	prep	in,role
R7469	T11683	T11684	nmod	motility,remodeling
R747	T1679	T1671	advmod	however,recruit
R7470	T11684	T11682	pobj	remodeling,in
R7471	T11685	T11683	cc	and,motility
R7472	T11686	T11683	conj	epithelial,motility
R7473	T11687	T11675	punct	", ",differ
R7474	T11688	T11675	nsubj	they,differ
R7475	T11689	T11675	prep	with,differ
R7476	T11690	T11689	pobj	respect,with
R7477	T11691	T11690	prep	to,respect
R7478	T11692	T11693	poss	Snail,effects
R7479	T11693	T11691	pobj	effects,to
R748	T1570	T1571	det	these,pathways
R7480	T11694	T11692	case	's,Snail
R7481	T11695	T11693	amod	apparent,effects
R7482	T11696	T11693	amod	proliferative,effects
R7483	T11697	T11675	punct	.,differ
R7484	T11699	T11700	advmod	A,priori
R7485	T11700	T11701	advmod	priori,be
R7486	T11702	T11701	punct	", ",be
R7487	T11703	T11701	nsubj	this,be
R7488	T11704	T11701	aux	could,be
R7489	T11705	T11701	advmod	simply,be
R749	T1680	T1671	punct	", ",recruit
R7490	T11706	T11701	prep	due,be
R7491	T11707	T11706	pcomp	to,due
R7492	T11708	T11706	pobj	variations,due
R7493	T11709	T11708	prep	in,variations
R7494	T11710	T11711	det	the,response
R7495	T11711	T11709	pobj	response,in
R7496	T11712	T11711	prep	of,response
R7497	T11713	T11714	amod	different,types
R7498	T11714	T11712	pobj	types,of
R7499	T11715	T11714	compound	cell,types
R750	T1571	T1569	pobj	pathways,of
R7500	T11716	T11711	prep	to,response
R7501	T11717	T11718	compound	Snail,expression
R7502	T11718	T11716	pobj	expression,to
R7503	T11719	T11701	punct	.,be
R7504	T11721	T11722	advmod	Alternatively,be
R7505	T11723	T11722	punct	", ",be
R7506	T11724	T11725	det	these,differences
R7507	T11725	T11722	nsubj	differences,be
R7508	T11726	T11722	aux	may,be
R7509	T11727	T11722	acomp	relevant,be
R751	T1681	T1682	nmod	E,cadherin
R7510	T11728	T11727	prep	to,relevant
R7511	T11729	T11730	det	the,benefit
R7512	T11730	T11728	pobj	benefit,to
R7513	T11731	T11730	prep	of,benefit
R7514	T11732	T11731	pcomp	using,of
R7515	T11733	T11734	compound	mouse,models
R7516	T11734	T11732	dobj	models,using
R7517	T11735	T11736	aux	to,reveal
R7518	T11736	T11732	advcl	reveal,using
R7519	T11737	T11736	dobj	functions,reveal
R752	T1572	T1571	nummod	two,pathways
R7520	T11738	T11739	neg	not,recapitulated
R7521	T11739	T11737	acl	recapitulated,functions
R7522	T11740	T11739	advmod	always,recapitulated
R7523	T11741	T11739	prep	in,recapitulated
R7524	T11742	T11743	amod	stable,models
R7525	T11743	T11741	pobj	models,in
R7526	T11744	T11745	compound	cell,line
R7527	T11745	T11743	compound	line,models
R7528	T11746	T11722	punct	.,be
R7529	T11748	T11749	amod	Future,studies
R753	T1573	T1571	amod	early,pathways
R7530	T11749	T11750	nsubj	studies,highlight
R7531	T11751	T11750	aux	should,highlight
R7532	T11752	T11753	det	the,reasons
R7533	T11753	T11750	dobj	reasons,highlight
R7534	T11754	T11753	amod	underlying,reasons
R7535	T11755	T11753	prep	for,reasons
R7536	T11756	T11757	det	these,results
R7537	T11757	T11755	pobj	results,for
R7538	T11758	T11757	amod	opposing,results
R7539	T11759	T11750	punct	.,highlight
R754	T1682	T1684	nmod	cadherin,complexes
R7540	T11761	T11762	advcl	Irrespective,stand
R7541	T11763	T11761	prep	of,Irrespective
R7542	T11764	T11765	det	these,differences
R7543	T11765	T11763	pobj	differences,of
R7544	T11766	T11762	punct	", ",stand
R7545	T11767	T11768	poss	our,studies
R7546	T11768	T11762	nsubj	studies,stand
R7547	T11769	T11770	advmod	in,vivo
R7548	T11770	T11768	amod	vivo,studies
R7549	T11771	T11762	aux	do,stand
R755	T1574	T1571	compound	signaling,pathways
R7550	T11772	T11762	neg	not,stand
R7551	T11773	T11762	advmod	alone,stand
R7552	T11774	T11762	punct	", ",stand
R7553	T11775	T11776	mark	as,are
R7554	T11776	T11762	advcl	are,stand
R7555	T11777	T11776	expl	there,are
R7556	T11778	T11779	amod	many,situations
R7557	T11779	T11776	attr	situations,are
R7558	T11780	T11781	prep	in,correlate
R7559	T11781	T11779	relcl	correlate,situations
R756	T1575	T1576	amod	down,regulation
R7560	T11782	T11780	pobj	which,in
R7561	T11783	T11784	det	a,regulation
R7562	T11784	T11781	nsubj	regulation,correlate
R7563	T11785	T11784	amod	down,regulation
R7564	T11786	T11784	punct	-,regulation
R7565	T11787	T11784	prep	in,regulation
R7566	T11788	T11789	compound	AJ,proteins
R7567	T11789	T11787	pobj	proteins,in
R7568	T11790	T11781	prep	with,correlate
R7569	T11791	T11792	amod	enhanced,proliferation
R757	T1683	T1682	punct	-,cadherin
R7570	T11792	T11790	pobj	proliferation,with
R7571	T11793	T11762	punct	.,stand
R7572	T11795	T11796	prep	In,implicated
R7573	T11797	T11795	pobj	fact,In
R7574	T11798	T11796	punct	", ",implicated
R7575	T11799	T11800	det	a,myriad
R7576	T11800	T11796	nsubjpass	myriad,implicated
R7577	T11801	T11800	prep	of,myriad
R7578	T11802	T11803	amod	diverse,mechanisms
R7579	T11803	T11801	pobj	mechanisms,of
R758	T1576	T1565	dobj	regulation,include
R7580	T11804	T11796	aux	have,implicated
R7581	T11805	T11796	auxpass	been,implicated
R7582	T11806	T11796	prep	in,implicated
R7583	T11807	T11806	pcomp	activating,in
R7584	T11808	T11809	amod	epithelial,proliferation
R7585	T11809	T11807	dobj	proliferation,activating
R7586	T11810	T11807	prep	upon,activating
R7587	T11811	T11812	amod	down,regulation
R7588	T11812	T11810	pobj	regulation,upon
R7589	T11813	T11812	punct	-,regulation
R759	T1577	T1576	punct	-,regulation
R7590	T11814	T11812	prep	of,regulation
R7591	T11815	T11816	compound	AJ,proteins
R7592	T11816	T11814	pobj	proteins,of
R7593	T11817	T11818	punct	[,48
R7594	T11818	T11796	parataxis	48,implicated
R7595	T11819	T11818	nummod	7,48
R7596	T11820	T11818	punct	",",48
R7597	T11821	T11818	nummod	23,48
R7598	T11822	T11818	punct	",",48
R7599	T11823	T11818	nummod	24,48
R760	T1578	T1576	prep	of,regulation
R7600	T11824	T11818	punct	",",48
R7601	T11825	T11818	punct	],48
R7602	T11826	T11796	punct	.,implicated
R7603	T11828	T11829	csubj	Sifting,is
R7604	T11830	T11828	prep	through,Sifting
R7605	T11831	T11832	det	these,pathways
R7606	T11832	T11830	pobj	pathways,through
R7607	T11833	T11832	amod	converging,pathways
R7608	T11834	T11829	acomp	likely,is
R7609	T11835	T11836	aux	to,be
R761	T1684	T1671	nsubj	complexes,recruit
R7610	T11836	T11834	xcomp	be,likely
R7611	T11837	T11838	det	a,process
R7612	T11838	T11836	attr	process,be
R7613	T11839	T11838	amod	difficult,process
R7614	T11840	T11839	cc	and,difficult
R7615	T11841	T11839	conj	painstaking,difficult
R7616	T11842	T11829	punct	.,is
R7617	T11844	T11845	nsubj	This,said
R7618	T11845	T11846	advcl	said,begin
R7619	T11847	T11846	punct	", ",begin
R762	T1579	T1580	det	the,gene
R7620	T11848	T11846	prep	by,begin
R7621	T11849	T11848	pcomp	identifying,by
R7622	T11850	T11851	det	the,status
R7623	T11851	T11849	dobj	status,identifying
R7624	T11852	T11851	prep	of,status
R7625	T11853	T11854	amod	different,players
R7626	T11854	T11852	pobj	players,of
R7627	T11855	T11854	acl	involved,players
R7628	T11856	T11849	prep	in,identifying
R7629	T11857	T11858	amod	specific,types
R763	T1580	T1578	pobj	gene,of
R7630	T11858	T11856	pobj	types,in
R7631	T11859	T11858	compound	cell,types
R7632	T11860	T11856	cc	and,in
R7633	T11861	T11856	conj	at,in
R7634	T11862	T11863	amod	specific,stages
R7635	T11863	T11861	pobj	stages,at
R7636	T11864	T11863	prep	in,stages
R7637	T11865	T11864	pobj	development,in
R7638	T11866	T11846	punct	", ",begin
R7639	T11867	T11868	poss	our,understanding
R764	T1685	T1682	punct	-,cadherin
R7640	T11868	T11846	nsubj	understanding,begin
R7641	T11869	T11868	amod	mechanistic,understanding
R7642	T11870	T11868	prep	of,understanding
R7643	T11871	T11872	advmod	how,linked
R7644	T11872	T11870	pcomp	linked,of
R7645	T11873	T11874	amod	intercellular,remodeling
R7646	T11874	T11872	nsubjpass	remodeling,linked
R7647	T11875	T11872	auxpass	is,linked
R7648	T11876	T11872	prep	to,linked
R7649	T11877	T11876	pobj	proliferation,to
R765	T1581	T1580	acl	encoding,gene
R7650	T11878	T11872	prep	in,linked
R7651	T11879	T11880	amod	epithelial,morphogenesis
R7652	T11880	T11878	pobj	morphogenesis,in
R7653	T11881	T11846	aux	should,begin
R7654	T11882	T11883	aux	to,surface
R7655	T11883	T11846	xcomp	surface,begin
R7656	T11884	T11883	prep	in,surface
R7657	T11885	T11886	det	the,future
R7658	T11886	T11884	pobj	future,in
R7659	T11887	T11846	punct	.,begin
R766	T1582	T1583	compound	E,cadherin
R7660	T11889	T11890	csubj	Elucidating,is
R7661	T11891	T11892	det	the,mechanisms
R7662	T11892	T11889	dobj	mechanisms,Elucidating
R7663	T11893	T11892	amod	molecular,mechanisms
R7664	T11894	T11895	prep	through,converge
R7665	T11895	T11892	relcl	converge,mechanisms
R7666	T11896	T11894	pobj	which,through
R7667	T11897	T11898	det	these,networks
R7668	T11898	T11895	nsubj	networks,converge
R7669	T11899	T11890	advmod	also,is
R767	T1583	T1581	dobj	cadherin,encoding
R7670	T11900	T11901	det	a,prerequisite
R7671	T11901	T11890	attr	prerequisite,is
R7672	T11902	T11901	prep	for,prerequisite
R7673	T11903	T11902	pcomp	understanding,for
R7674	T11904	T11905	advmod	how,go
R7675	T11905	T11903	ccomp	go,understanding
R7676	T11906	T11907	det	these,processes
R7677	T11907	T11905	nsubj	processes,go
R7678	T11908	T11905	advmod	awry,go
R7679	T11909	T11905	prep	during,go
R768	T1686	T1687	compound	β,catenin
R7680	T11910	T11909	pobj	tumorigenesis,during
R7681	T11911	T11890	punct	.,is
R7682	T12023	T12024	amod	Primary,antibodies
R7683	T12024	T12025	nsubj	antibodies,were
R7684	T12026	T12024	acl	used,antibodies
R7685	T12027	T12025	prep	against,were
R7686	T12028	T12027	punct	: ,against
R7687	T12029	T12030	compound	E,cadherin
R7688	T12030	T12027	pobj	cadherin,against
R7689	T12031	T12030	punct	-,cadherin
R769	T1584	T1583	punct	-,cadherin
R7690	T12032	T12033	punct	(,Takeichi
R7691	T12033	T12030	parataxis	Takeichi,cadherin
R7692	T12034	T12033	compound	M.,Takeichi
R7693	T12035	T12033	punct	", ",Takeichi
R7694	T12036	T12037	compound	Kyoto,University
R7695	T12037	T12033	npadvmod	University,Takeichi
R7696	T12038	T12033	punct	", ",Takeichi
R7697	T12039	T12033	npadvmod	Japan,Takeichi
R7698	T12040	T12033	punct	),Takeichi
R7699	T12041	T12030	punct	;,cadherin
R770	T1585	T1583	punct	", ",cadherin
R7700	T12042	T12043	compound	α,catenin
R7701	T12043	T12030	conj	catenin,cadherin
R7702	T12044	T12043	punct	-,catenin
R7703	T12045	T12043	punct	", ",catenin
R7704	T12046	T12047	compound	β,catenin
R7705	T12047	T12043	appos	catenin,catenin
R7706	T12048	T12047	punct	-,catenin
R7707	T12049	T12043	punct	", ",catenin
R7708	T12050	T12043	appos	pMAPK,catenin
R7709	T12051	T12043	punct	", ",catenin
R771	T1586	T1587	det	the,cadherin
R7710	T12052	T12043	appos	tubulin,catenin
R7711	T12053	T12054	punct	(,Sigma
R7712	T12054	T12043	parataxis	Sigma,catenin
R7713	T12055	T12054	punct	", ",Sigma
R7714	T12056	T12057	compound	St.,Louis
R7715	T12057	T12054	npadvmod	Louis,Sigma
R7716	T12058	T12054	punct	", ",Sigma
R7717	T12059	T12054	npadvmod	Missouri,Sigma
R7718	T12060	T12054	punct	", ",Sigma
R7719	T12061	T12062	compound	United,States
R772	T1687	T1682	appos	catenin,cadherin
R7720	T12062	T12054	npadvmod	States,Sigma
R7721	T12063	T12054	punct	),Sigma
R7722	T12064	T12043	punct	", ",catenin
R7723	T12065	T12043	conj	Ajuba,catenin
R7724	T12066	T12067	punct	(,Longmore
R7725	T12067	T12065	parataxis	Longmore,Ajuba
R7726	T12068	T12067	compound	G.,Longmore
R7727	T12069	T12067	punct	", ",Longmore
R7728	T12070	T12071	compound	Washington,University
R7729	T12071	T12067	npadvmod	University,Longmore
R773	T1587	T1583	appos	cadherin,cadherin
R7730	T12072	T12067	punct	", ",Longmore
R7731	T12073	T12074	compound	St.,Louis
R7732	T12074	T12067	npadvmod	Louis,Longmore
R7733	T12075	T12067	punct	", ",Longmore
R7734	T12076	T12067	npadvmod	Missouri,Longmore
R7735	T12077	T12067	punct	", ",Longmore
R7736	T12078	T12079	compound	United,States
R7737	T12079	T12067	npadvmod	States,Longmore
R7738	T12080	T12067	punct	),Longmore
R7739	T12081	T12065	punct	;,Ajuba
R774	T1588	T1587	amod	prototypical,cadherin
R7740	T12082	T12083	compound	β4,integrin
R7741	T12083	T12065	conj	integrin,Ajuba
R7742	T12084	T12083	punct	/,integrin
R7743	T12085	T12083	appos	CD104,integrin
R7744	T12086	T12087	punct	(,Pharmingen
R7745	T12087	T12083	parataxis	Pharmingen,integrin
R7746	T12088	T12087	compound	BD,Pharmingen
R7747	T12089	T12087	punct	", ",Pharmingen
R7748	T12090	T12091	compound	San,Diego
R7749	T12091	T12087	npadvmod	Diego,Pharmingen
R775	T1688	T1687	punct	-,catenin
R7750	T12092	T12087	punct	", ",Pharmingen
R7751	T12093	T12087	npadvmod	California,Pharmingen
R7752	T12094	T12087	punct	", ",Pharmingen
R7753	T12095	T12096	compound	United,States
R7754	T12096	T12087	npadvmod	States,Pharmingen
R7755	T12097	T12087	punct	),Pharmingen
R7756	T12098	T12083	punct	", ",integrin
R7757	T12099	T12083	conj	laminin,integrin
R7758	T12100	T12099	nummod	5,laminin
R7759	T12101	T12102	punct	(,Burgeson
R776	T1589	T1587	amod	epithelial,cadherin
R7760	T12102	T12099	parataxis	Burgeson,laminin
R7761	T12103	T12102	compound	R.,Burgeson
R7762	T12104	T12102	punct	", ",Burgeson
R7763	T12105	T12106	compound	Harvard,University
R7764	T12106	T12102	npadvmod	University,Burgeson
R7765	T12107	T12102	punct	", ",Burgeson
R7766	T12108	T12102	npadvmod	Cambridge,Burgeson
R7767	T12109	T12102	punct	", ",Burgeson
R7768	T12110	T12102	npadvmod	Massachusetts,Burgeson
R7769	T12111	T12102	punct	", ",Burgeson
R777	T1590	T1591	dep	that,forms
R7770	T12112	T12113	compound	United,States
R7771	T12113	T12102	npadvmod	States,Burgeson
R7772	T12114	T12102	punct	),Burgeson
R7773	T12115	T12099	punct	", ",laminin
R7774	T12116	T12099	conj	K5,laminin
R7775	T12117	T12116	punct	", ",K5
R7776	T12118	T12116	conj	K1,K5
R7777	T12119	T12118	punct	", ",K1
R7778	T12120	T12118	conj	loricrin,K1
R7779	T12121	T12122	punct	(,Lab
R778	T1591	T1587	relcl	forms,cadherin
R7780	T12122	T12120	parataxis	Lab,loricrin
R7781	T12123	T12122	compound	Fuchs,Lab
R7782	T12124	T12122	punct	),Lab
R7783	T12125	T12120	punct	", ",loricrin
R7784	T12126	T12120	conj	involucrin,loricrin
R7785	T12127	T12126	punct	", ",involucrin
R7786	T12128	T12126	conj	fillagrin,involucrin
R7787	T12129	T12130	punct	(,Covance
R7788	T12130	T12128	parataxis	Covance,fillagrin
R7789	T12131	T12130	punct	", ",Covance
R779	T1689	T1690	compound	α,catenin
R7790	T12132	T12130	npadvmod	Berkeley,Covance
R7791	T12133	T12130	punct	", ",Covance
R7792	T12134	T12130	npadvmod	California,Covance
R7793	T12135	T12130	punct	", ",Covance
R7794	T12136	T12137	compound	United,States
R7795	T12137	T12130	npadvmod	States,Covance
R7796	T12138	T12130	punct	),Covance
R7797	T12139	T12128	punct	", ",fillagrin
R7798	T12140	T12128	conj	MAPK,fillagrin
R7799	T12141	T12140	punct	", ",MAPK
R780	T1592	T1593	det	the,core
R7800	T12142	T12140	conj	pSMAD2,MAPK
R7801	T12143	T12144	punct	(,Signaling
R7802	T12144	T12142	parataxis	Signaling,pSMAD2
R7803	T12145	T12144	compound	Cell,Signaling
R7804	T12146	T12144	punct	", ",Signaling
R7805	T12147	T12144	npadvmod	Beverly,Signaling
R7806	T12148	T12144	punct	", ",Signaling
R7807	T12149	T12144	npadvmod	Massachusetts,Signaling
R7808	T12150	T12144	punct	", ",Signaling
R7809	T12151	T12152	compound	United,States
R781	T1593	T1591	dobj	core,forms
R7810	T12152	T12144	npadvmod	States,Signaling
R7811	T12153	T12144	punct	),Signaling
R7812	T12154	T12142	punct	;,pSMAD2
R7813	T12155	T12142	conj	Grb,pSMAD2
R7814	T12156	T12155	punct	-,Grb
R7815	T12157	T12155	nummod	2,Grb
R7816	T12158	T12159	punct	(,Biotech
R7817	T12159	T12155	parataxis	Biotech,Grb
R7818	T12160	T12161	compound	Santa,Cruz
R7819	T12161	T12159	compound	Cruz,Biotech
R782	T1690	T1671	dobj	catenin,recruit
R7820	T12162	T12159	punct	", ",Biotech
R7821	T12163	T12164	compound	Santa,Cruz
R7822	T12164	T12159	npadvmod	Cruz,Biotech
R7823	T12165	T12159	punct	", ",Biotech
R7824	T12166	T12159	npadvmod	California,Biotech
R7825	T12167	T12159	punct	", ",Biotech
R7826	T12168	T12169	compound	United,States
R7827	T12169	T12159	npadvmod	States,Biotech
R7828	T12170	T12159	punct	),Biotech
R7829	T12171	T12155	punct	;,Grb
R783	T1594	T1593	amod	transmembrane,core
R7830	T12172	T12173	compound	P,cadherin
R7831	T12173	T12155	conj	cadherin,Grb
R7832	T12174	T12173	punct	-,cadherin
R7833	T12175	T12176	punct	(,Laboratories
R7834	T12176	T12173	parataxis	Laboratories,cadherin
R7835	T12177	T12176	compound	Zymed,Laboratories
R7836	T12178	T12176	punct	", ",Laboratories
R7837	T12179	T12180	amod	South,Francisco
R7838	T12180	T12176	npadvmod	Francisco,Laboratories
R7839	T12181	T12180	compound	San,Francisco
R784	T1595	T1593	prep	of,core
R7840	T12182	T12176	punct	", ",Laboratories
R7841	T12183	T12176	npadvmod	California,Laboratories
R7842	T12184	T12176	punct	", ",Laboratories
R7843	T12185	T12186	compound	United,States
R7844	T12186	T12176	npadvmod	States,Laboratories
R7845	T12187	T12176	punct	),Laboratories
R7846	T12188	T12173	punct	;,cadherin
R7847	T12189	T12173	conj	HA,cadherin
R7848	T12190	T12191	punct	(,Biochemicals
R7849	T12191	T12189	parataxis	Biochemicals,HA
R785	T1596	T1597	amod	intercellular,junctions
R7850	T12192	T12191	compound	Roche,Biochemicals
R7851	T12193	T12191	punct	),Biochemicals
R7852	T12194	T12189	punct	", ",HA
R7853	T12195	T12189	conj	vimentin,HA
R7854	T12196	T12197	punct	(,Chemicon
R7855	T12197	T12195	parataxis	Chemicon,vimentin
R7856	T12198	T12197	punct	", ",Chemicon
R7857	T12199	T12197	npadvmod	Temecula,Chemicon
R7858	T12200	T12197	punct	", ",Chemicon
R7859	T12201	T12197	npadvmod	California,Chemicon
R786	T1691	T1690	punct	-,catenin
R7860	T12202	T12197	punct	", ",Chemicon
R7861	T12203	T12204	compound	United,States
R7862	T12204	T12197	npadvmod	States,Chemicon
R7863	T12205	T12197	punct	),Chemicon
R7864	T12206	T12195	punct	", ",vimentin
R7865	T12207	T12195	conj	Ki67,vimentin
R7866	T12208	T12209	punct	(,Castra
R7867	T12209	T12207	parataxis	Castra,Ki67
R7868	T12210	T12209	compound	Novo,Castra
R7869	T12211	T12209	punct	", ",Castra
R787	T1597	T1595	pobj	junctions,of
R7870	T12212	T12209	npadvmod	Newcastle,Castra
R7871	T12213	T12212	prep	Upon,Newcastle
R7872	T12214	T12213	pobj	Tyne,Upon
R7873	T12215	T12209	punct	", ",Castra
R7874	T12216	T12217	compound	United,Kingdom
R7875	T12217	T12209	npadvmod	Kingdom,Castra
R7876	T12218	T12209	punct	),Castra
R7877	T12219	T12207	punct	", ",Ki67
R7878	T12220	T12207	conj	keratin,Ki67
R7879	T12221	T12220	nummod	6,keratin
R788	T1598	T1597	compound	adherens,junctions
R7880	T12222	T12223	punct	(,Coulombe
R7881	T12223	T12220	parataxis	Coulombe,keratin
R7882	T12224	T12223	compound	P.,Coulombe
R7883	T12225	T12223	punct	", ",Coulombe
R7884	T12226	T12227	compound	John,Hopkins
R7885	T12227	T12228	compound	Hopkins,University
R7886	T12228	T12223	npadvmod	University,Coulombe
R7887	T12229	T12223	punct	", ",Coulombe
R7888	T12230	T12223	npadvmod	Baltimore,Coulombe
R7889	T12231	T12223	punct	", ",Coulombe
R789	T1599	T1597	punct	(,junctions
R7890	T12232	T12223	npadvmod	Maryland,Coulombe
R7891	T12233	T12223	punct	", ",Coulombe
R7892	T12234	T12235	compound	United,States
R7893	T12235	T12223	npadvmod	States,Coulombe
R7894	T12236	T12223	punct	),Coulombe
R7895	T12237	T12220	punct	", ",keratin
R7896	T12238	T12239	compound	cyclin,D
R7897	T12239	T12220	conj	D,keratin
R7898	T12240	T12241	punct	(,Oncogene
R7899	T12241	T12239	parataxis	Oncogene,D
R790	T1692	T1690	punct	", ",catenin
R7900	T12242	T12241	punct	", ",Oncogene
R7901	T12243	T12244	compound	San,Diego
R7902	T12244	T12241	npadvmod	Diego,Oncogene
R7903	T12245	T12241	punct	", ",Oncogene
R7904	T12246	T12241	npadvmod	California,Oncogene
R7905	T12247	T12241	punct	", ",Oncogene
R7906	T12248	T12249	compound	United,States
R7907	T12249	T12241	npadvmod	States,Oncogene
R7908	T12250	T12241	punct	),Oncogene
R7909	T12251	T12239	punct	", ",D
R791	T1600	T1597	appos	AJs,junctions
R7910	T12252	T12239	cc	and,D
R7911	T12253	T12254	compound	TGF,β2
R7912	T12254	T12239	conj	β2,D
R7913	T12255	T12254	punct	-,β2
R7914	T12256	T12257	punct	(,Gold
R7915	T12257	T12254	parataxis	Gold,β2
R7916	T12258	T12257	compound	L.,Gold
R7917	T12259	T12257	punct	", ",Gold
R7918	T12260	T12261	compound	New,York
R7919	T12261	T12262	compound	York,University
R792	T1601	T1565	punct	),include
R7920	T12262	T12257	npadvmod	University,Gold
R7921	T12263	T12257	punct	", ",Gold
R7922	T12264	T12265	compound	New,York
R7923	T12265	T12257	npadvmod	York,Gold
R7924	T12266	T12257	punct	", ",Gold
R7925	T12267	T12268	compound	New,York
R7926	T12268	T12257	npadvmod	York,Gold
R7927	T12269	T12257	punct	", ",Gold
R7928	T12270	T12271	compound	United,States
R7929	T12271	T12257	npadvmod	States,Gold
R793	T1602	T1603	punct	[,5
R7930	T12272	T12257	punct	),Gold
R7931	T12273	T12025	punct	.,were
R7932	T12275	T12276	npadvmod	FITC,conjugated
R7933	T12276	T12286	amod	conjugated,antibodies
R7934	T12277	T12275	punct	-,FITC
R7935	T12278	T12275	punct	", ",FITC
R7936	T12279	T12280	compound	Texas,Red
R7937	T12280	T12275	conj	Red,FITC
R7938	T12281	T12280	punct	-,Red
R7939	T12282	T12280	punct	", ",Red
R794	T1603	T1565	parataxis	5,include
R7940	T12283	T12280	cc	or,Red
R7941	T12284	T12280	conj	HRP,Red
R7942	T12285	T12276	punct	-,conjugated
R7943	T12286	T12288	nsubj	antibodies,were
R7944	T12287	T12286	amod	secondary,antibodies
R7945	T12289	T12288	prep	from,were
R7946	T12290	T12291	compound	Jackson,ImmunoResearch
R7947	T12291	T12289	pobj	ImmunoResearch,from
R7948	T12292	T12293	punct	(,Grove
R7949	T12293	T12291	parataxis	Grove,ImmunoResearch
R795	T1604	T1603	punct	],5
R7950	T12294	T12293	compound	West,Grove
R7951	T12295	T12293	punct	", ",Grove
R7952	T12296	T12293	npadvmod	Pennsylvania,Grove
R7953	T12297	T12293	punct	", ",Grove
R7954	T12298	T12299	compound	United,States
R7955	T12299	T12293	npadvmod	States,Grove
R7956	T12300	T12293	punct	),Grove
R7957	T12301	T12288	punct	.,were
R7958	T12303	T12304	amod	Biotinylated,antibodies
R7959	T12304	T12306	nsubj	antibodies,were
R796	T1693	T1694	dep	which,coordinates
R7960	T12305	T12304	amod	secondary,antibodies
R7961	T12307	T12306	prep	from,were
R7962	T12308	T12309	compound	Vector,Labs
R7963	T12309	T12307	pobj	Labs,from
R7964	T12310	T12311	punct	(,Burlingame
R7965	T12311	T12309	parataxis	Burlingame,Labs
R7966	T12312	T12311	punct	", ",Burlingame
R7967	T12313	T12311	npadvmod	California,Burlingame
R7968	T12314	T12311	punct	", ",Burlingame
R7969	T12315	T12316	compound	United,States
R797	T1605	T1565	punct	.,include
R7970	T12316	T12311	npadvmod	States,Burlingame
R7971	T12317	T12311	punct	),Burlingame
R7972	T12318	T12306	punct	.,were
R7973	T12320	T12321	nsubj	Dilutions,were
R7974	T12322	T12321	prep	according,were
R7975	T12323	T12322	prep	to,according
R7976	T12324	T12325	det	the,manufacturer
R7977	T12325	T12326	poss	manufacturer,recommendation
R7978	T12326	T12323	pobj	recommendation,to
R7979	T12327	T12325	case	's,manufacturer
R798	T1607	T1608	nsubj	We,showed
R7980	T12328	T12321	punct	.,were
R7981	T12330	T12331	det	The,antibody
R7982	T12331	T12333	nsubjpass	antibody,generated
R7983	T12332	T12331	compound	Snail,antibody
R7984	T12334	T12333	auxpass	was,generated
R7985	T12335	T12333	prep	in,generated
R7986	T12336	T12337	compound	Guinea,pigs
R7987	T12337	T12335	pobj	pigs,in
R7988	T12338	T12333	prep	by,generated
R7989	T12339	T12338	pcomp	inoculating,by
R799	T1694	T1690	relcl	coordinates,catenin
R7990	T12340	T12339	dobj	them,inoculating
R7991	T12341	T12339	prep	with,inoculating
R7992	T12342	T12343	det	the,sequence
R7993	T12343	T12341	pobj	sequence,with
R7994	T12344	T12345	npadvmod	N,terminal
R7995	T12345	T12343	amod	terminal,sequence
R7996	T12346	T12345	punct	-,terminal
R7997	T12347	T12343	prep	of,sequence
R7998	T12348	T12349	amod	murine,Snail
R7999	T12349	T12347	pobj	Snail,of
R800	T1609	T1608	advmod	subsequently,showed
R8000	T12350	T12343	acl	fused,sequence
R8001	T12351	T12350	prep	to,fused
R8002	T12352	T12351	pobj	GST,to
R8003	T12353	T12354	punct	(,Covance
R8004	T12354	T12352	parataxis	Covance,GST
R8005	T12355	T12354	punct	", ",Covance
R8006	T12356	T12354	npadvmod	Princeton,Covance
R8007	T12357	T12354	punct	", ",Covance
R8008	T12358	T12359	compound	New,Jersey
R8009	T12359	T12354	npadvmod	Jersey,Covance
R801	T1695	T1694	prep	in,coordinates
R8010	T12360	T12354	punct	", ",Covance
R8011	T12361	T12362	compound	United,States
R8012	T12362	T12354	npadvmod	States,Covance
R8013	T12363	T12354	punct	),Covance
R8014	T12364	T12333	punct	.,generated
R8015	T12366	T12367	amod	Recombinant,β2
R8016	T12367	T12371	nsubjpass	β2,purchased
R8017	T12368	T12367	amod	human,β2
R8018	T12369	T12367	compound	TGF,β2
R8019	T12370	T12367	punct	-,β2
R802	T1610	T1611	mark	that,blocked
R8020	T12372	T12371	auxpass	was,purchased
R8021	T12373	T12371	prep	from,purchased
R8022	T12374	T12373	pobj	R,from
R8023	T12375	T12374	cc	&,R
R8024	T12376	T12374	conj	D,R
R8025	T12377	T12378	punct	(,Minneapolis
R8026	T12378	T12376	parataxis	Minneapolis,D
R8027	T12379	T12378	punct	", ",Minneapolis
R8028	T12380	T12378	npadvmod	Minnesota,Minneapolis
R8029	T12381	T12378	punct	", ",Minneapolis
R803	T1696	T1695	pobj	turn,in
R8030	T12382	T12383	compound	United,States
R8031	T12383	T12378	npadvmod	States,Minneapolis
R8032	T12384	T12378	punct	),Minneapolis
R8033	T12385	T12371	punct	.,purchased
R8034	T12387	T12388	npadvmod	Heat,inactivated
R8035	T12388	T12389	amod	inactivated,β2
R8036	T12389	T12392	nsubjpass	β2,generated
R8037	T12390	T12389	compound	TGF,β2
R8038	T12391	T12389	punct	-,β2
R8039	T12393	T12392	auxpass	was,generated
R804	T1611	T1608	ccomp	blocked,showed
R8040	T12394	T12392	prep	by,generated
R8041	T12395	T12394	pcomp	heating,by
R8042	T12396	T12397	det	the,protein
R8043	T12397	T12395	dobj	protein,heating
R8044	T12398	T12397	amod	recombinant,protein
R8045	T12399	T12395	prep	at,heating
R8046	T12400	T12401	nummod	100,°C
R8047	T12401	T12399	pobj	°C,at
R8048	T12402	T12395	prep	for,heating
R8049	T12403	T12404	nummod	10,min
R805	T1697	T1698	det	the,dynamics
R8050	T12404	T12402	pobj	min,for
R8051	T12405	T12392	punct	.,generated
R8052	T12466	T12467	det	The,mouse
R8053	T12467	T12472	nsubjpass	mouse,generated
R8054	T12468	T12469	compound	K14,Snail
R8055	T12469	T12467	compound	Snail,mouse
R8056	T12470	T12469	punct	-,Snail
R8057	T12471	T12467	compound	Tg,mouse
R8058	T12473	T12472	auxpass	was,generated
R8059	T12474	T12472	prep	by,generated
R806	T1612	T1613	advmod	when,elevated
R8060	T12475	T12474	pcomp	digesting,by
R8061	T12476	T12477	det	the,plasmid
R8062	T12477	T12475	dobj	plasmid,digesting
R8063	T12478	T12479	compound	pcDNA3,mm
R8064	T12479	T12477	compound	mm,plasmid
R8065	T12480	T12479	punct	-,mm
R8066	T12481	T12482	compound	Snail,HA
R8067	T12482	T12477	compound	HA,plasmid
R8068	T12483	T12482	punct	-,HA
R8069	T12484	T12485	punct	(,Herreros
R807	T1613	T1611	advcl	elevated,blocked
R8070	T12485	T12477	parataxis	Herreros,plasmid
R8071	T12486	T12485	compound	G.,Herreros
R8072	T12487	T12485	compound	de,Herreros
R8073	T12488	T12485	punct	", ",Herreros
R8074	T12489	T12490	compound	Universitat,Pompeu
R8075	T12490	T12485	npadvmod	Pompeu,Herreros
R8076	T12491	T12485	punct	", ",Herreros
R8077	T12492	T12485	npadvmod	Fabra,Herreros
R8078	T12493	T12485	punct	", ",Herreros
R8079	T12494	T12485	npadvmod	Barcelona,Herreros
R808	T1698	T1694	dobj	dynamics,coordinates
R8080	T12495	T12485	punct	", ",Herreros
R8081	T12496	T12485	npadvmod	Spain,Herreros
R8082	T12497	T12485	punct	),Herreros
R8083	T12498	T12475	prep	with,digesting
R8084	T12499	T12498	pobj	BamHI,with
R8085	T12500	T12499	cc	and,BamHI
R8086	T12501	T12499	conj	NotI,BamHI
R8087	T12502	T12472	cc	and,generated
R8088	T12503	T12472	conj	subcloned,generated
R8089	T12504	T12503	prep	into,subcloned
R809	T1614	T1615	compound	E,cadherin
R8090	T12505	T12506	det	the,vector
R8091	T12506	T12504	pobj	vector,into
R8092	T12507	T12506	compound	K14,vector
R8093	T12508	T12509	punct	[,49
R8094	T12509	T12503	parataxis	49,subcloned
R8095	T12510	T12509	punct	],49
R8096	T12511	T12472	punct	.,generated
R8097	T12513	T12514	det	The,construct
R8098	T12514	T12516	nsubjpass	construct,injected
R8099	T12515	T12514	amod	linearized,construct
R810	T1615	T1613	nsubjpass	cadherin,elevated
R8100	T12517	T12516	auxpass	was,injected
R8101	T12518	T12516	prep	into,injected
R8102	T12519	T12520	det	the,nucleus
R8103	T12520	T12518	pobj	nucleus,into
R8104	T12521	T12520	prep	of,nucleus
R8105	T12522	T12521	pobj	embryos,of
R8106	T12523	T12522	prep	from,embryos
R8107	T12524	T12525	compound	CD1,mice
R8108	T12525	T12523	pobj	mice,from
R8109	T12526	T12516	punct	.,injected
R811	T1616	T1615	punct	-,cadherin
R8110	T12528	T12529	det	The,mouse
R8111	T12529	T12535	nsubjpass	mouse,reported
R8112	T12530	T12531	nmod	K14,Smad
R8113	T12531	T12529	nmod	Smad,mouse
R8114	T12532	T12531	punct	-,Smad
R8115	T12533	T12534	nummod	2,Tg
R8116	T12534	T12529	compound	Tg,mouse
R8117	T12536	T12535	auxpass	was,reported
R8118	T12537	T12535	prep	in,reported
R8119	T12538	T12537	pobj	Ito,in
R812	T1699	T1698	amod	associated,dynamics
R8120	T12539	T12540	advmod	et,al.
R8121	T12540	T12538	advmod	al.,Ito
R8122	T12541	T12538	punct	", ",Ito
R8123	T12542	T12538	npadvmod	2001,Ito
R8124	T12543	T12535	punct	.,reported
R8125	T12545	T12546	det	The,mouse
R8126	T12546	T12554	nsubjpass	mouse,described
R8127	T12547	T12548	nmod	TGF,β2
R8128	T12548	T12546	nmod	β2,mouse
R8129	T12549	T12548	punct	-,β2
R813	T1617	T1613	auxpass	is,elevated
R8130	T12550	T12548	appos	knockout,β2
R8131	T12551	T12550	punct	(,knockout
R8132	T12552	T12550	appos	KO,knockout
R8133	T12553	T12546	punct	),mouse
R8134	T12555	T12554	auxpass	was,described
R8135	T12556	T12554	prep	in,described
R8136	T12557	T12558	punct	[,34
R8137	T12558	T12556	pobj	34,in
R8138	T12559	T12554	punct	],described
R8139	T12560	T12554	punct	.,described
R814	T1618	T1613	advmod	transgenically,elevated
R8140	T12562	T12563	det	The,mouse
R8141	T12563	T12566	nsubjpass	mouse,reported
R8142	T12564	T12565	compound	shh,KO
R8143	T12565	T12563	compound	KO,mouse
R8144	T12567	T12568	punct	[,38
R8145	T12568	T12563	parataxis	38,mouse
R8146	T12569	T12568	punct	],38
R8147	T12570	T12563	cc	and,mouse
R8148	T12571	T12572	compound	TOPGal,mouse
R8149	T12572	T12563	conj	mouse,mouse
R815	T1700	T1698	compound	actin,dynamics
R8150	T12573	T12574	punct	[,20
R8151	T12574	T12572	parataxis	20,mouse
R8152	T12575	T12574	punct	],20
R8153	T12576	T12566	aux	have,reported
R8154	T12577	T12566	advmod	previously,reported
R8155	T12578	T12566	auxpass	been,reported
R8156	T12579	T12566	punct	.,reported
R8157	T12687	T12688	compound	Western,blot
R8158	T12689	T12688	cc	and,blot
R8159	T12690	T12688	conj	immunoprecipitation,blot
R816	T1619	T1613	prep	in,elevated
R8160	T12692	T12693	compound	Protein,extracts
R8161	T12693	T12694	nsubjpass	extracts,generated
R8162	T12695	T12693	prep	from,extracts
R8163	T12696	T12697	amod	primary,keratinocytes
R8164	T12697	T12695	pobj	keratinocytes,from
R8165	T12698	T12694	auxpass	were,generated
R8166	T12699	T12700	preconj	either,by
R8167	T12700	T12694	prep	by,generated
R8168	T12701	T12700	pcomp	lysing,by
R8169	T12702	T12701	dobj	cells,lysing
R817	T1620	T1621	compound	mouse,skin
R8170	T12703	T12701	prep	in,lysing
R8171	T12704	T12705	compound	lysis,buffer
R8172	T12705	T12703	pobj	buffer,in
R8173	T12706	T12707	punct	(,NP
R8174	T12707	T12705	parataxis	NP,buffer
R8175	T12708	T12709	nummod	1,%
R8176	T12709	T12707	compound	%,NP
R8177	T12710	T12707	punct	-,NP
R8178	T12711	T12707	nummod	40,NP
R8179	T12712	T12707	punct	", ",NP
R818	T1621	T1619	pobj	skin,in
R8180	T12713	T12714	nummod	1,%
R8181	T12714	T12715	compound	%,deoxycholate
R8182	T12715	T12707	conj	deoxycholate,NP
R8183	T12716	T12715	compound	sodium,deoxycholate
R8184	T12717	T12715	punct	", ",deoxycholate
R8185	T12718	T12719	nummod	20,mM
R8186	T12719	T12720	compound	mM,Cl
R8187	T12720	T12715	conj	Cl,deoxycholate
R8188	T12721	T12720	compound	Tris,Cl
R8189	T12722	T12720	punct	-,Cl
R819	T1701	T1698	compound	polymerization,dynamics
R8190	T12723	T12720	punct	[,Cl
R8191	T12724	T12720	npadvmod	pH,Cl
R8192	T12725	T12724	nummod	7.4,pH
R8193	T12726	T12720	punct	],Cl
R8194	T12727	T12720	punct	", ",Cl
R8195	T12728	T12729	nummod	140,mM
R8196	T12729	T12730	compound	mM,NaCl
R8197	T12730	T12720	conj	NaCl,Cl
R8198	T12731	T12730	acl	containing,NaCl
R8199	T12732	T12733	nummod	1,mM
R820	T1622	T1611	punct	", ",blocked
R8200	T12733	T12734	compound	mM,vanadate
R8201	T12734	T12731	dobj	vanadate,containing
R8202	T12735	T12734	compound	sodium,vanadate
R8203	T12736	T12730	punct	", ",NaCl
R8204	T12737	T12738	nummod	2,mM
R8205	T12738	T12739	compound	mM,fluoride
R8206	T12739	T12730	conj	fluoride,NaCl
R8207	T12740	T12739	compound	phenylmethylsulfonyl,fluoride
R8208	T12741	T12739	punct	", ",fluoride
R8209	T12742	T12739	cc	and,fluoride
R821	T1623	T1624	compound	hair,follicle
R8210	T12743	T12744	compound	protease,inhibitors
R8211	T12744	T12739	conj	inhibitors,fluoride
R8212	T12745	T12707	punct	),NP
R8213	T12746	T12703	cc	or,in
R8214	T12747	T12748	advmod	directly,in
R8215	T12748	T12703	conj	in,in
R8216	T12749	T12750	compound	Laemmli,bβuffer
R8217	T12750	T12748	pobj	bβuffer,in
R8218	T12751	T12694	cc	and,generated
R8219	T12752	T12694	conj	boiled,generated
R822	T1624	T1625	compound	follicle,morphogenesis
R8220	T12753	T12694	punct	.,generated
R8221	T12755	T12756	prep	For,pulverized
R8222	T12757	T12758	compound	skin,tissue
R8223	T12758	T12755	pobj	tissue,For
R8224	T12759	T12756	punct	: ,pulverized
R8225	T12760	T12761	amod	Frozen,tissue
R8226	T12761	T12756	nsubjpass	tissue,pulverized
R8227	T12762	T12756	auxpass	was,pulverized
R8228	T12763	T12756	prep	in,pulverized
R8229	T12764	T12765	det	a,Gevebesmascher
R823	T1702	T1698	amod	necessary,dynamics
R8230	T12765	T12763	pobj	Gevebesmascher,in
R8231	T12766	T12767	amod	liquid,nitrogen
R8232	T12767	T12768	npadvmod	nitrogen,cooled
R8233	T12768	T12765	amod	cooled,Gevebesmascher
R8234	T12769	T12768	punct	-,cooled
R8235	T12770	T12756	cc	and,pulverized
R8236	T12771	T12772	det	the,powder
R8237	T12772	T12773	nsubj	powder,scraped
R8238	T12773	T12756	conj	scraped,pulverized
R8239	T12774	T12773	prep	into,scraped
R824	T1625	T1611	nsubjpass	morphogenesis,blocked
R8240	T12775	T12776	det	a,tube
R8241	T12776	T12774	pobj	tube,into
R8242	T12777	T12776	amod	chilled,tube
R8243	T12778	T12776	compound	microcentrifuge,tube
R8244	T12779	T12756	punct	.,pulverized
R8245	T12781	T12782	compound	RIPA,buffer
R8246	T12782	T12783	nsubjpass	buffer,added
R8247	T12784	T12785	punct	(,X
R8248	T12785	T12782	parataxis	X,buffer
R8249	T12786	T12787	nummod	1,%
R825	T1626	T1611	auxpass	is,blocked
R8250	T12787	T12785	compound	%,X
R8251	T12788	T12785	compound	Triton,X
R8252	T12789	T12785	punct	-,X
R8253	T12790	T12785	nummod	100,X
R8254	T12791	T12785	prep	in,X
R8255	T12792	T12791	pobj	PBS,in
R8256	T12793	T12785	prep	with,X
R8257	T12794	T12795	nummod	10,mM
R8258	T12795	T12796	compound	mM,EDTA
R8259	T12796	T12793	pobj	EDTA,with
R826	T1627	T1611	punct	", ",blocked
R8260	T12797	T12796	punct	", ",EDTA
R8261	T12798	T12799	nummod	150,mN
R8262	T12799	T12800	compound	mN,NaCl
R8263	T12800	T12796	conj	NaCl,EDTA
R8264	T12801	T12800	punct	", ",NaCl
R8265	T12802	T12803	nummod	1,%
R8266	T12803	T12804	compound	%,deoxycholate
R8267	T12804	T12800	conj	deoxycholate,NaCl
R8268	T12805	T12804	compound	sodium,deoxycholate
R8269	T12806	T12804	punct	", ",deoxycholate
R827	T1703	T1704	aux	to,stabilize
R8270	T12807	T12804	cc	and,deoxycholate
R8271	T12808	T12809	nummod	0.1,%
R8272	T12809	T12810	compound	%,SDS
R8273	T12810	T12804	conj	SDS,deoxycholate
R8274	T12811	T12785	punct	),X
R8275	T12812	T12782	cc	and,buffer
R8276	T12813	T12814	compound	protease,inhibitors
R8277	T12814	T12782	conj	inhibitors,buffer
R8278	T12815	T12814	cc	or,inhibitors
R8279	T12816	T12817	compound	Laemmli,buffer
R828	T1628	T1611	advcl	suggesting,blocked
R8280	T12817	T12814	conj	buffer,inhibitors
R8281	T12818	T12783	auxpass	was,added
R8282	T12819	T12783	punct	.,added
R8283	T12821	T12822	det	The,suspension
R8284	T12822	T12824	nsubjpass	suspension,sonicated
R8285	T12823	T12822	compound	cell,suspension
R8286	T12825	T12824	auxpass	was,sonicated
R8287	T12826	T12827	nummod	three,times
R8288	T12827	T12824	npadvmod	times,sonicated
R8289	T12828	T12824	prep	for,sonicated
R829	T1629	T1630	mark	that,is
R8290	T12829	T12830	nummod	15,s
R8291	T12830	T12828	pobj	s,for
R8292	T12831	T12824	cc	and,sonicated
R8293	T12832	T12824	conj	centrifuged,sonicated
R8294	T12833	T12832	prep	at,centrifuged
R8295	T12834	T12835	nummod	"14,000",rpm
R8296	T12835	T12833	pobj	rpm,at
R8297	T12836	T12832	prep	at,centrifuged
R8298	T12837	T12838	nummod	4,°C
R8299	T12838	T12836	pobj	°C,at
R830	T1630	T1628	ccomp	is,suggesting
R8300	T12839	T12824	punct	.,sonicated
R8301	T12841	T12842	det	The,supernatant
R8302	T12842	T12843	nsubjpass	supernatant,separated
R8303	T12844	T12843	auxpass	was,separated
R8304	T12845	T12843	prep	from,separated
R8305	T12846	T12847	det	the,pellet
R8306	T12847	T12845	pobj	pellet,from
R8307	T12848	T12843	cc	and,separated
R8308	T12849	T12843	conj	used,separated
R8309	T12850	T12849	prep	in,used
R831	T1704	T1702	xcomp	stabilize,necessary
R8310	T12851	T12852	det	the,experiments
R8311	T12852	T12850	pobj	experiments,in
R8312	T12853	T12843	punct	.,separated
R8313	T12855	T12856	nsubjpass	Extracts,precleared
R8314	T12857	T12855	acl	subjected,Extracts
R8315	T12858	T12857	prep	to,subjected
R8316	T12859	T12858	pobj	immunoprecipitation,to
R8317	T12860	T12856	auxpass	were,precleared
R8318	T12861	T12856	prep	with,precleared
R8319	T12862	T12863	compound	Protein,G
R832	T1631	T1632	nmod	E,cadherin
R8320	T12863	T12864	compound	G,Sepharose
R8321	T12864	T12861	pobj	Sepharose,with
R8322	T12865	T12866	punct	(,Amersham
R8323	T12866	T12864	parataxis	Amersham,Sepharose
R8324	T12867	T12866	punct	", ",Amersham
R8325	T12868	T12866	npadvmod	Piscataway,Amersham
R8326	T12869	T12866	punct	", ",Amersham
R8327	T12870	T12871	compound	New,York
R8328	T12871	T12866	npadvmod	York,Amersham
R8329	T12872	T12866	punct	", ",Amersham
R833	T1632	T1634	nmod	cadherin,regulation
R8330	T12873	T12874	compound	United,States
R8331	T12874	T12866	npadvmod	States,Amersham
R8332	T12875	T12866	punct	),Amersham
R8333	T12876	T12856	cc	and,precleared
R8334	T12877	T12856	conj	incubated,precleared
R8335	T12878	T12877	prep	with,incubated
R8336	T12879	T12878	pobj	antibody,with
R8337	T12880	T12877	prep	with,incubated
R8338	T12881	T12880	pobj	rocking,with
R8339	T12882	T12877	advmod	overnight,incubated
R834	T1705	T1706	amod	nascent,AJs
R8340	T12883	T12877	prep	at,incubated
R8341	T12884	T12885	nummod	4,°C
R8342	T12885	T12883	pobj	°C,at
R8343	T12886	T12856	punct	.,precleared
R8344	T12888	T12889	compound	Protein,G
R8345	T12889	T12890	compound	G,Sepharose
R8346	T12890	T12891	nsubjpass	Sepharose,added
R8347	T12892	T12891	auxpass	was,added
R8348	T12893	T12891	cc	and,added
R8349	T12894	T12895	nsubjpass	samples,incubated
R835	T1633	T1632	punct	-,cadherin
R8350	T12895	T12891	conj	incubated,added
R8351	T12896	T12895	auxpass	were,incubated
R8352	T12897	T12895	prep	for,incubated
R8353	T12898	T12899	nummod	1,h
R8354	T12899	T12897	pobj	h,for
R8355	T12900	T12895	prep	at,incubated
R8356	T12901	T12902	nummod	4,°C
R8357	T12902	T12900	pobj	°C,at
R8358	T12903	T12895	prep	with,incubated
R8359	T12904	T12903	pobj	rocking,with
R836	T1634	T1630	nsubj	regulation,is
R8360	T12905	T12895	punct	.,incubated
R8361	T12907	T12908	nsubjpass	Samples,washed
R8362	T12909	T12908	auxpass	were,washed
R8363	T12910	T12911	nummod	three,times
R8364	T12911	T12908	npadvmod	times,washed
R8365	T12912	T12908	prep	for,washed
R8366	T12913	T12914	nummod	5,min
R8367	T12914	T12912	pobj	min,for
R8368	T12915	T12914	advmod	each,min
R8369	T12916	T12908	prep	in,washed
R837	T1635	T1634	amod	down,regulation
R8370	T12917	T12918	compound	lysis,buffer
R8371	T12918	T12916	pobj	buffer,in
R8372	T12919	T12908	punct	", ",washed
R8373	T12920	T12908	cc	and,washed
R8374	T12921	T12922	det	the,pellet
R8375	T12922	T12930	nsubjpass	pellet,resuspended
R8376	T12923	T12924	compound	Protein,G
R8377	T12924	T12925	compound	G,antigen
R8378	T12925	T12922	compound	antigen,pellet
R8379	T12926	T12925	compound	Sepharose,antigen
R838	T1706	T1704	dobj	AJs,stabilize
R8380	T12927	T12925	punct	-,antigen
R8381	T12928	T12925	compound	antibody,antigen
R8382	T12929	T12925	punct	-,antigen
R8383	T12930	T12908	conj	resuspended,washed
R8384	T12931	T12930	auxpass	was,resuspended
R8385	T12932	T12930	prep	in,resuspended
R8386	T12933	T12934	compound	Laemmli,buffer
R8387	T12934	T12932	pobj	buffer,in
R8388	T12935	T12930	cc	and,resuspended
R8389	T12936	T12930	conj	boiled,resuspended
R839	T1636	T1634	punct	-,regulation
R8390	T12937	T12936	prep	for,boiled
R8391	T12938	T12939	nummod	10,min
R8392	T12939	T12937	pobj	min,for
R8393	T12940	T12930	punct	.,resuspended
R8394	T12942	T12943	nsubjpass	Samples,run
R8395	T12944	T12943	auxpass	were,run
R8396	T12945	T12943	prep	on,run
R8397	T12946	T12947	compound	SDS,PAGE
R8398	T12947	T12945	pobj	PAGE,on
R8399	T12948	T12947	punct	-,PAGE
R840	T1637	T1638	det	a,event
R8400	T12949	T12943	cc	and,run
R8401	T12950	T12943	conj	transferred,run
R8402	T12951	T12950	prep	to,transferred
R8403	T12952	T12953	compound	nitrocellulose,membrane
R8404	T12953	T12951	pobj	membrane,to
R8405	T12954	T12955	punct	(,Bioscience
R8406	T12955	T12953	parataxis	Bioscience,membrane
R8407	T12956	T12955	nmod	Schleicher,Bioscience
R8408	T12957	T12956	cc	and,Schleicher
R8409	T12958	T12956	conj	Schuell,Schleicher
R841	T1638	T1630	attr	event,is
R8410	T12959	T12955	punct	", ",Bioscience
R8411	T12960	T12955	npadvmod	Keene,Bioscience
R8412	T12961	T12955	punct	", ",Bioscience
R8413	T12962	T12963	compound	New,Hampshire
R8414	T12963	T12955	npadvmod	Hampshire,Bioscience
R8415	T12964	T12955	punct	", ",Bioscience
R8416	T12965	T12966	compound	United,States
R8417	T12966	T12955	npadvmod	States,Bioscience
R8418	T12967	T12955	punct	),Bioscience
R8419	T12968	T12943	punct	.,run
R842	T1639	T1638	amod	critical,event
R8420	T12970	T12971	compound	Western,blot
R8421	T12971	T12972	compound	blot,signals
R8422	T12972	T12973	nsubjpass	signals,developed
R8423	T12974	T12973	auxpass	were,developed
R8424	T12975	T12973	advcl	using,developed
R8425	T12976	T12977	det	the,kit
R8426	T12977	T12975	dobj	kit,using
R8427	T12978	T12977	amod	enhanced,kit
R8428	T12979	T12977	compound	chemiluminescence,kit
R8429	T12980	T12977	prep	from,kit
R843	T1640	T1638	prep	in,event
R8430	T12981	T12980	pobj	Amersham,from
R8431	T13041	T13042	compound	Cell,culture
R8432	T13044	T13045	amod	Primary,keratinocytes
R8433	T13045	T13046	nsubjpass	keratinocytes,culture
R8434	T13047	T13046	auxpass	were,culture
R8435	T13048	T13046	prep	in,culture
R8436	T13049	T13050	amod	low,calcium
R8437	T13050	T13052	compound	calcium,medium
R8438	T13051	T13050	punct	-,calcium
R8439	T13052	T13048	pobj	medium,in
R844	T1707	T1704	cc	and,stabilize
R8440	T13053	T13054	mark	as,described
R8441	T13054	T13046	advcl	described,culture
R8442	T13055	T13054	advmod	previously,described
R8443	T13056	T13057	punct	[,4
R8444	T13057	T13046	parataxis	4,culture
R8445	T13058	T13057	punct	],4
R8446	T13059	T13046	punct	.,culture
R8447	T13061	T13062	amod	Transient,transfections
R8448	T13062	T13063	nsubjpass	transfections,carried
R8449	T13064	T13063	auxpass	were,carried
R845	T1708	T1704	conj	integrate,stabilize
R8450	T13065	T13063	prt	out,carried
R8451	T13066	T13063	prep	with,carried
R8452	T13067	T13068	compound	FuGENE6,reagent
R8453	T13068	T13066	pobj	reagent,with
R8454	T13069	T13070	punct	(,Roche
R8455	T13070	T13068	parataxis	Roche,reagent
R8456	T13071	T13070	punct	", ",Roche
R8457	T13072	T13070	npadvmod	Indianapolis,Roche
R8458	T13073	T13070	punct	", ",Roche
R8459	T13074	T13070	npadvmod	Indiana,Roche
R846	T1641	T1640	pcomp	governing,in
R8460	T13075	T13070	punct	", ",Roche
R8461	T13076	T13077	compound	United,States
R8462	T13077	T13070	npadvmod	States,Roche
R8463	T13078	T13070	punct	),Roche
R8464	T13079	T13063	prep	according,carried
R8465	T13080	T13079	prep	to,according
R8466	T13081	T13082	det	the,manufacturer
R8467	T13082	T13083	poss	manufacturer,protocol
R8468	T13083	T13080	pobj	protocol,to
R8469	T13084	T13082	case	's,manufacturer
R847	T1709	T1710	det	the,cytoskeleton
R8470	T13085	T13063	punct	.,carried
R8471	T13087	T13088	nsubjpass	Measurement,carried
R8472	T13089	T13087	prep	of,Measurement
R8473	T13090	T13091	nmod	β,galactosidase
R8474	T13091	T13093	nmod	galactosidase,levels
R8475	T13092	T13091	punct	-,galactosidase
R8476	T13093	T13089	pobj	levels,of
R8477	T13094	T13091	cc	or,galactosidase
R8478	T13095	T13091	conj	luciferase,galactosidase
R8479	T13096	T13088	prep	in,carried
R848	T1642	T1643	det	the,dynamics
R8480	T13097	T13098	compound	promoter,activity
R8481	T13098	T13099	compound	activity,studies
R8482	T13099	T13096	pobj	studies,in
R8483	T13100	T13088	auxpass	were,carried
R8484	T13101	T13088	prt	out,carried
R8485	T13102	T13088	prep	with,carried
R8486	T13103	T13104	det	the,kit
R8487	T13104	T13102	pobj	kit,with
R8488	T13105	T13106	compound	Galacto,Lite
R8489	T13106	T13104	compound	Lite,kit
R849	T1643	T1641	dobj	dynamics,governing
R8490	T13107	T13106	punct	-,Lite
R8491	T13108	T13104	compound	assay,kit
R8492	T13109	T13110	punct	(,TROPIX
R8493	T13110	T13104	parataxis	TROPIX,kit
R8494	T13111	T13110	punct	", ",TROPIX
R8495	T13112	T13110	npadvmod	Bedford,TROPIX
R8496	T13113	T13110	punct	", ",TROPIX
R8497	T13114	T13110	npadvmod	Massachusetts,TROPIX
R8498	T13115	T13110	punct	", ",TROPIX
R8499	T13116	T13117	compound	United,States
R850	T1644	T1643	compound	adhesion,dynamics
R8500	T13117	T13110	npadvmod	States,TROPIX
R8501	T13118	T13110	punct	),TROPIX
R8502	T13119	T13104	cc	and,kit
R8503	T13120	T13121	det	the,luciferase
R8504	T13121	T13104	conj	luciferase,kit
R8505	T13122	T13121	amod	Dual,luciferase
R8506	T13123	T13124	punct	(,Promega
R8507	T13124	T13121	parataxis	Promega,luciferase
R8508	T13125	T13124	punct	", ",Promega
R8509	T13126	T13124	npadvmod	Madison,Promega
R851	T1710	T1708	dobj	cytoskeleton,integrate
R8510	T13127	T13124	punct	", ",Promega
R8511	T13128	T13124	npadvmod	Wisconsin,Promega
R8512	T13129	T13124	punct	", ",Promega
R8513	T13130	T13131	compound	United,States
R8514	T13131	T13124	npadvmod	States,Promega
R8515	T13132	T13124	punct	),Promega
R8516	T13133	T13121	punct	", ",luciferase
R8517	T13134	T13088	advmod	respectively,carried
R8518	T13135	T13088	punct	.,carried
R8519	T13137	T13138	compound	Runella,luciferase
R852	T1645	T1643	amod	necessary,dynamics
R8520	T13138	T13139	nsubjpass	luciferase,cotransfected
R8521	T13140	T13139	auxpass	was,cotransfected
R8522	T13141	T13139	prep	into,cotransfected
R8523	T13142	T13141	pobj	cells,into
R8524	T13143	T13144	aux	to,correct
R8525	T13144	T13139	advcl	correct,cotransfected
R8526	T13145	T13144	prep	for,correct
R8527	T13146	T13147	compound	transfection,efficiency
R8528	T13147	T13145	pobj	efficiency,for
R8529	T13148	T13139	punct	.,cotransfected
R853	T1646	T1645	prep	for,necessary
R8530	T13150	T13151	nsubjpass	Experiments,done
R8531	T13152	T13151	auxpass	were,done
R8532	T13153	T13151	prep	in,done
R8533	T13154	T13153	pobj	triplicate,in
R8534	T13155	T13151	cc	and,done
R8535	T13156	T13151	conj	repeated,done
R8536	T13157	T13158	advmod	at,three
R8537	T13158	T13160	nummod	three,times
R8538	T13159	T13158	advmod	least,three
R8539	T13160	T13156	npadvmod	times,repeated
R854	T1647	T1648	compound	budding,morphogenesis
R8540	T13161	T13151	punct	.,done
R8541	T13163	T13164	nsubjpass	Measurements,done
R8542	T13165	T13164	auxpass	were,done
R8543	T13166	T13164	prep	on,done
R8544	T13167	T13168	det	a,luminometer
R8545	T13168	T13166	pobj	luminometer,on
R8546	T13169	T13170	punct	(,Instruments
R8547	T13170	T13168	parataxis	Instruments,luminometer
R8548	T13171	T13170	compound	MGM,Instruments
R8549	T13172	T13170	punct	", ",Instruments
R855	T1711	T1708	prep	across,integrate
R8550	T13173	T13170	npadvmod	Hamden,Instruments
R8551	T13174	T13170	punct	", ",Instruments
R8552	T13175	T13170	npadvmod	Connecticut,Instruments
R8553	T13176	T13170	punct	", ",Instruments
R8554	T13177	T13178	compound	United,States
R8555	T13178	T13170	npadvmod	States,Instruments
R8556	T13179	T13170	punct	),Instruments
R8557	T13180	T13164	punct	.,done
R8558	T13182	T13183	prep	For,starved
R8559	T13184	T13182	pobj	experiments,For
R856	T1648	T1646	pobj	morphogenesis,for
R8560	T13185	T13184	acl	measuring,experiments
R8561	T13186	T13185	dobj	phosphorylation,measuring
R8562	T13187	T13186	prep	of,phosphorylation
R8563	T13188	T13187	pobj	MAPK,of
R8564	T13189	T13183	punct	", ",starved
R8565	T13190	T13183	nsubjpass	keratinocytes,starved
R8566	T13191	T13183	auxpass	were,starved
R8567	T13192	T13183	dep	serum,starved
R8568	T13193	T13183	prep	for,starved
R8569	T13194	T13195	nummod	3,h
R857	T1649	T1650	punct	[,4
R8570	T13195	T13193	pobj	h,for
R8571	T13196	T13197	amod	prior,to
R8572	T13197	T13183	prep	to,starved
R8573	T13198	T13197	pobj	harvesting,to
R8574	T13199	T13198	prep	of,harvesting
R8575	T13200	T13199	pobj	cells,of
R8576	T13201	T13198	prep	by,harvesting
R8577	T13202	T13201	pobj	incubation,by
R8578	T13203	T13202	prep	in,incubation
R8579	T13204	T13203	pobj	medium,in
R858	T1712	T1713	det	an,sheet
R8580	T13205	T13204	acl	lacking,medium
R8581	T13206	T13205	dobj	serum,lacking
R8582	T13207	T13183	punct	.,starved
R8583	T13209	T13210	nsubjpass	Treatment,described
R8584	T13211	T13209	prep	of,Treatment
R8585	T13212	T13211	pobj	cells,of
R8586	T13213	T13209	prep	with,Treatment
R8587	T13214	T13215	npadvmod	Wnt,conditioned
R8588	T13215	T13220	amod	conditioned,medium
R8589	T13216	T13214	punct	-,Wnt
R859	T1650	T1608	parataxis	4,showed
R8590	T13217	T13214	cc	and,Wnt
R8591	T13218	T13214	conj	noggin,Wnt
R8592	T13219	T13215	punct	-,conditioned
R8593	T13220	T13213	pobj	medium,with
R8594	T13221	T13210	auxpass	was,described
R8595	T13222	T13210	advmod	previously,described
R8596	T13223	T13224	punct	[,4
R8597	T13224	T13210	parataxis	4,described
R8598	T13225	T13224	punct	],4
R8599	T13226	T13210	punct	.,described
R860	T1651	T1650	punct	],4
R8600	T13310	T13311	det	The,promoter
R8601	T13311	T13317	nsubjpass	promoter,generated
R8602	T13312	T13313	nummod	2.2,kb
R8603	T13313	T13311	nmod	kb,promoter
R8604	T13314	T13313	punct	-,kb
R8605	T13315	T13311	amod	murine,promoter
R8606	T13316	T13311	compound	Snail,promoter
R8607	T13318	T13317	auxpass	was,generated
R8608	T13319	T13317	prep	by,generated
R8609	T13320	T13319	pobj	PCR,by
R861	T1652	T1608	punct	.,showed
R8610	T13321	T13317	advcl	using,generated
R8611	T13322	T13323	det	a,primer
R8612	T13323	T13321	dobj	primer,using
R8613	T13324	T13323	amod	forward,primer
R8614	T13325	T13321	prep	with,using
R8615	T13326	T13327	det	an,sequence
R8616	T13327	T13325	pobj	sequence,with
R8617	T13328	T13327	compound	XbaI,sequence
R8618	T13329	T13327	compound	linker,sequence
R8619	T13330	T13327	punct	", ",sequence
R862	T1713	T1711	pobj	sheet,across
R8620	T13331	T13332	nummod	5,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8621	T13332	T13327	appos	TCTAGAATTGTTTGCTGCTGTATGGTCTTC,sequence
R8622	T13333	T13331	punct	′,5
R8623	T13334	T13332	punct	-,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8624	T13335	T13332	punct	-,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8625	T13336	T13332	nummod	3,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8626	T13337	T13332	punct	′,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8627	T13338	T13327	punct	", ",sequence
R8628	T13339	T13327	cc	along,sequence
R8629	T13340	T13339	dep	with,along
R863	T1654	T1655	prep	Like,binds
R8630	T13341	T13342	det	a,primer
R8631	T13342	T13327	conj	primer,sequence
R8632	T13343	T13342	amod	reverse,primer
R8633	T13344	T13342	prep	with,primer
R8634	T13345	T13346	det	a,sequence
R8635	T13346	T13344	pobj	sequence,with
R8636	T13347	T13346	compound	BglII,sequence
R8637	T13348	T13346	compound	linker,sequence
R8638	T13349	T13346	punct	", ",sequence
R8639	T13350	T13351	nummod	5,GTAG
R864	T1714	T1713	amod	epithelial,sheet
R8640	T13351	T13346	appos	GTAG,sequence
R8641	T13352	T13350	punct	′,5
R8642	T13353	T13351	punct	-,GTAG
R8643	T13354	T13351	compound	AGATCTGTTGGCCAGAGCGACCTAG,GTAG
R8644	T13355	T13351	punct	-,GTAG
R8645	T13356	T13351	punct	-,GTAG
R8646	T13357	T13351	nummod	3,GTAG
R8647	T13358	T13351	punct	′,GTAG
R8648	T13359	T13342	punct	", ",primer
R8649	T13360	T13342	cc	and,primer
R865	T1656	T1654	pobj	LEF,Like
R8650	T13361	T13362	nmod	mouse,DNA
R8651	T13362	T13342	conj	DNA,primer
R8652	T13363	T13362	amod	genomic,DNA
R8653	T13364	T13362	prep	as,DNA
R8654	T13365	T13366	det	a,template
R8655	T13366	T13364	pobj	template,as
R8656	T13367	T13317	punct	.,generated
R8657	T13369	T13370	det	The,product
R8658	T13370	T13372	nsubjpass	product,purified
R8659	T13371	T13370	compound	PCR,product
R866	T1657	T1656	punct	-,LEF
R8660	T13373	T13372	auxpass	was,purified
R8661	T13374	T13372	prep	with,purified
R8662	T13375	T13376	det	the,Kit
R8663	T13376	T13374	pobj	Kit,with
R8664	T13377	T13378	compound	Gel,Extraction
R8665	T13378	T13376	compound	Extraction,Kit
R8666	T13379	T13380	punct	(,Qiagen
R8667	T13380	T13376	parataxis	Qiagen,Kit
R8668	T13381	T13380	punct	", ",Qiagen
R8669	T13382	T13380	npadvmod	Valencia,Qiagen
R867	T1715	T1716	punct	[,8
R8670	T13383	T13380	punct	", ",Qiagen
R8671	T13384	T13380	npadvmod	California,Qiagen
R8672	T13385	T13380	punct	", ",Qiagen
R8673	T13386	T13387	compound	United,States
R8674	T13387	T13380	npadvmod	States,Qiagen
R8675	T13388	T13380	punct	),Qiagen
R8676	T13389	T13372	cc	and,purified
R8677	T13390	T13372	conj	ligated,purified
R8678	T13391	T13390	prep	into,ligated
R8679	T13392	T13393	compound	pCRII,TOPO
R868	T1658	T1656	nummod	1,LEF
R8680	T13393	T13395	compound	TOPO,vector
R8681	T13394	T13393	punct	-,TOPO
R8682	T13395	T13391	pobj	vector,into
R8683	T13396	T13395	compound	TA,vector
R8684	T13397	T13398	punct	(,Invitrogen
R8685	T13398	T13395	parataxis	Invitrogen,vector
R8686	T13399	T13398	punct	", ",Invitrogen
R8687	T13400	T13398	npadvmod	Carlsbad,Invitrogen
R8688	T13401	T13398	punct	", ",Invitrogen
R8689	T13402	T13398	npadvmod	California,Invitrogen
R869	T1659	T1655	punct	", ",binds
R8690	T13403	T13398	punct	", ",Invitrogen
R8691	T13404	T13405	compound	United,States
R8692	T13405	T13398	npadvmod	States,Invitrogen
R8693	T13406	T13398	punct	),Invitrogen
R8694	T13407	T13372	punct	.,purified
R8695	T13409	T13410	det	The,promoter
R8696	T13410	T13411	nsubjpass	promoter,verified
R8697	T13412	T13411	auxpass	was,verified
R8698	T13413	T13411	prep	by,verified
R8699	T13414	T13413	pobj	sequencing,by
R870	T1660	T1661	compound	E,cadherin
R8700	T13415	T13411	cc	and,verified
R8701	T13416	T13411	conj	digested,verified
R8702	T13417	T13416	prep	with,digested
R8703	T13418	T13417	pobj	XbaI,with
R8704	T13419	T13418	cc	and,XbaI
R8705	T13420	T13418	conj	BglII,XbaI
R8706	T13421	T13416	cc	and,digested
R8707	T13422	T13416	conj	subcloned,digested
R8708	T13423	T13422	prep	into,subcloned
R8709	T13424	T13425	det	the,vector
R871	T1661	T1655	nsubj	cadherin,binds
R8710	T13425	T13423	pobj	vector,into
R8711	T13426	T13427	compound	pβ,gal
R8712	T13427	T13425	compound	gal,vector
R8713	T13428	T13427	punct	-,gal
R8714	T13429	T13425	compound	BASIC,vector
R8715	T13430	T13431	punct	(,Clontech
R8716	T13431	T13425	parataxis	Clontech,vector
R8717	T13432	T13431	compound	BD,Clontech
R8718	T13433	T13431	compound	Biosciences,Clontech
R8719	T13434	T13431	punct	", ",Clontech
R872	T1716	T1694	parataxis	8,coordinates
R8720	T13435	T13436	compound	Palo,Alto
R8721	T13436	T13431	npadvmod	Alto,Clontech
R8722	T13437	T13431	punct	", ",Clontech
R8723	T13438	T13431	npadvmod	California,Clontech
R8724	T13439	T13431	punct	", ",Clontech
R8725	T13440	T13441	compound	United,States
R8726	T13441	T13431	npadvmod	States,Clontech
R8727	T13442	T13431	punct	),Clontech
R8728	T13443	T13411	punct	.,verified
R8729	T13445	T13446	det	The,mutations
R873	T1662	T1661	punct	-,cadherin
R8730	T13446	T13448	nsubjpass	mutations,generated
R8731	T13447	T13446	compound	point,mutations
R8732	T13449	T13446	prep	in,mutations
R8733	T13450	T13451	det	the,element
R8734	T13451	T13449	pobj	element,in
R8735	T13452	T13451	compound	SMAD,element
R8736	T13453	T13451	compound	binding,element
R8737	T13454	T13448	auxpass	was,generated
R8738	T13455	T13448	prep	with,generated
R8739	T13456	T13457	det	the,Kit
R874	T1663	T1655	advmod	also,binds
R8740	T13457	T13455	pobj	Kit,with
R8741	T13458	T13459	compound	Quik,Change
R8742	T13459	T13457	compound	Change,Kit
R8743	T13460	T13459	punct	-,Change
R8744	T13461	T13462	punct	(,Stratagene
R8745	T13462	T13457	parataxis	Stratagene,Kit
R8746	T13463	T13462	punct	", ",Stratagene
R8747	T13464	T13465	compound	La,Jolla
R8748	T13465	T13462	npadvmod	Jolla,Stratagene
R8749	T13466	T13462	punct	", ",Stratagene
R875	T1717	T1716	nummod	6,8
R8750	T13467	T13462	npadvmod	California,Stratagene
R8751	T13468	T13462	punct	", ",Stratagene
R8752	T13469	T13470	compound	United,States
R8753	T13470	T13462	npadvmod	States,Stratagene
R8754	T13471	T13462	punct	),Stratagene
R8755	T13472	T13448	advcl	using,generated
R8756	T13473	T13474	det	the,primer
R8757	T13474	T13472	dobj	primer,using
R8758	T13475	T13474	amod	forward,primer
R8759	T13476	T13477	nummod	5,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R876	T1664	T1655	prep	to,binds
R8760	T13477	T13474	appos	GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC,primer
R8761	T13478	T13476	punct	′,5
R8762	T13479	T13477	punct	-,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8763	T13480	T13477	punct	-,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8764	T13481	T13477	nummod	3,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8765	T13482	T13477	punct	′,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8766	T13483	T13474	cc	and,primer
R8767	T13484	T13485	det	the,primer
R8768	T13485	T13474	conj	primer,primer
R8769	T13486	T13485	amod	reverse,primer
R877	T1665	T1666	compound	β,catenin
R8770	T13487	T13488	nummod	5,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8771	T13488	T13485	appos	CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC,primer
R8772	T13489	T13487	punct	′,5
R8773	T13490	T13488	punct	-,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8774	T13491	T13488	compound	GCTTTT,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8775	T13492	T13488	punct	-,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8776	T13493	T13488	punct	-,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8777	T13494	T13488	nummod	3,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8778	T13495	T13488	punct	′,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8779	T13496	T13448	punct	.,generated
R878	T1666	T1664	pobj	catenin,to
R8780	T13498	T13499	det	The,probes
R8781	T13499	T13500	nsubjpass	probes,generated
R8782	T13501	T13499	prep	for,probes
R8783	T13502	T13503	det	the,hybridization
R8784	T13503	T13501	pobj	hybridization,for
R8785	T13504	T13503	nmod	Snail,hybridization
R8786	T13505	T13506	advmod	in,situ
R8787	T13506	T13503	amod	situ,hybridization
R8788	T13507	T13500	auxpass	were,generated
R8789	T13508	T13500	prep	against,generated
R879	T1718	T1716	punct	",",8
R8790	T13509	T13510	det	the,UTR
R8791	T13510	T13508	pobj	UTR,against
R8792	T13511	T13510	nummod	3,UTR
R8793	T13512	T13511	punct	′,3
R8794	T13513	T13500	prep	by,generated
R8795	T13514	T13513	pobj	PCR,by
R8796	T13515	T13500	advcl	using,generated
R8797	T13516	T13517	det	the,primer
R8798	T13517	T13515	dobj	primer,using
R8799	T13518	T13517	amod	forward,primer
R880	T1667	T1666	punct	-,catenin
R8800	T13519	T13520	nummod	5,ACCTTCTCCCGCATGTCCTTGCTCC
R8801	T13520	T13517	appos	ACCTTCTCCCGCATGTCCTTGCTCC,primer
R8802	T13521	T13519	punct	′,5
R8803	T13522	T13520	punct	-,ACCTTCTCCCGCATGTCCTTGCTCC
R8804	T13523	T13520	punct	-,ACCTTCTCCCGCATGTCCTTGCTCC
R8805	T13524	T13520	nummod	3,ACCTTCTCCCGCATGTCCTTGCTCC
R8806	T13525	T13520	punct	′,ACCTTCTCCCGCATGTCCTTGCTCC
R8807	T13526	T13517	cc	and,primer
R8808	T13527	T13528	det	the,primer
R8809	T13528	T13517	conj	primer,primer
R881	T1668	T1655	punct	.,binds
R8810	T13529	T13528	amod	reverse,primer
R8811	T13530	T13531	nummod	5,CTGCTGAGGCATGGTTACAGCTGG
R8812	T13531	T13528	appos	CTGCTGAGGCATGGTTACAGCTGG,primer
R8813	T13532	T13530	punct	′,5
R8814	T13533	T13531	punct	-,CTGCTGAGGCATGGTTACAGCTGG
R8815	T13534	T13531	punct	-,CTGCTGAGGCATGGTTACAGCTGG
R8816	T13535	T13531	nummod	3,CTGCTGAGGCATGGTTACAGCTGG
R8817	T13536	T13531	punct	′,CTGCTGAGGCATGGTTACAGCTGG
R8818	T13537	T13528	punct	", ",primer
R8819	T13538	T13528	cc	and,primer
R882	T1719	T1716	nummod	7,8
R8820	T13539	T13540	amod	genomic,DNA
R8821	T13540	T13528	conj	DNA,primer
R8822	T13541	T13540	prep	as,DNA
R8823	T13542	T13543	det	a,template
R8824	T13543	T13541	pobj	template,as
R8825	T13544	T13500	punct	.,generated
R8826	T13546	T13547	det	The,product
R8827	T13547	T13549	nsubjpass	product,purified
R8828	T13548	T13547	compound	PCR,product
R8829	T13550	T13549	auxpass	was,purified
R883	T1670	T1671	prep	At,recruit
R8830	T13551	T13549	dep	gel,purified
R8831	T13552	T13549	cc	and,purified
R8832	T13553	T13549	conj	ligated,purified
R8833	T13554	T13553	prep	into,ligated
R8834	T13555	T13556	compound	pCRII,TOPO
R8835	T13556	T13558	compound	TOPO,vector
R8836	T13557	T13556	punct	-,TOPO
R8837	T13558	T13554	pobj	vector,into
R8838	T13559	T13558	compound	TA,vector
R8839	T13560	T13549	punct	.,purified
R884	T1720	T1716	punct	",",8
R8840	T13562	T13563	det	The,domain
R8841	T13563	T13565	nsubjpass	domain,generated
R8842	T13564	T13563	amod	pre-LIM,domain
R8843	T13566	T13563	prep	of,domain
R8844	T13567	T13566	pobj	Ajuba,of
R8845	T13568	T13565	auxpass	was,generated
R8846	T13569	T13565	advmod	essentially,generated
R8847	T13570	T13571	mark	as,described
R8848	T13571	T13565	advcl	described,generated
R8849	T13572	T13573	punct	[,9
R885	T1672	T1670	pobj	sites,At
R8850	T13573	T13571	parataxis	9,described
R8851	T13574	T13573	punct	],9
R8852	T13575	T13565	punct	", ",generated
R8853	T13576	T13565	cc	but,generated
R8854	T13577	T13578	auxpass	was,fused
R8855	T13578	T13565	conj	fused,generated
R8856	T13579	T13578	prep	to,fused
R8857	T13580	T13579	pobj	GFP,to
R8858	T13581	T13578	prep	by,fused
R8859	T13582	T13581	pcomp	subcloning,by
R886	T1673	T1672	prep	of,sites
R8860	T13583	T13582	prep	from,subcloning
R8861	T13584	T13585	det	the,vector
R8862	T13585	T13583	pobj	vector,from
R8863	T13586	T13587	nmod	pEGFP,N1
R8864	T13587	T13585	nmod	N1,vector
R8865	T13588	T13587	punct	-,N1
R8866	T13589	T13585	nummod	20,vector
R8867	T13590	T13591	punct	(,Clontech
R8868	T13591	T13585	parataxis	Clontech,vector
R8869	T13592	T13591	compound	BD,Clontech
R887	T1674	T1675	compound	cell,cell
R8870	T13593	T13591	compound	Biosciences,Clontech
R8871	T13594	T13591	punct	),Clontech
R8874	T13649	T13650	advmod	In,situ
R8875	T13650	T13651	amod	situ,hybridization
R8876	T13653	T13654	det	The,vector
R8877	T13654	T13659	nsubjpass	vector,used
R8878	T13655	T13656	compound	pCRII,TOPO
R8879	T13656	T13654	compound	TOPO,vector
R888	T1721	T1716	punct	],8
R8880	T13657	T13656	punct	-,TOPO
R8881	T13658	T13654	compound	TA,vector
R8882	T13660	T13654	acl	containing,vector
R8883	T13661	T13662	det	a,region
R8884	T13662	T13660	dobj	region,containing
R8885	T13663	T13662	prep	of,region
R8886	T13664	T13665	det	the,UTR
R8887	T13665	T13663	pobj	UTR,of
R8888	T13666	T13665	nummod	3,UTR
R8889	T13667	T13666	punct	′,3
R889	T1722	T1671	punct	.,recruit
R8890	T13668	T13665	prep	of,UTR
R8891	T13669	T13668	pobj	Snail,of
R8892	T13670	T13659	auxpass	was,used
R8893	T13671	T13659	prep	as,used
R8894	T13672	T13673	det	a,template
R8895	T13673	T13671	pobj	template,as
R8896	T13674	T13675	aux	to,generate
R8897	T13675	T13659	advcl	generate,used
R8898	T13676	T13677	npadvmod	digoxigenin,labeled
R8899	T13677	T13679	amod	labeled,riboprobes
R890	T1724	T1725	compound	α,Catenin
R8900	T13678	T13677	punct	-,labeled
R8901	T13679	T13675	dobj	riboprobes,generate
R8902	T13680	T13679	nmod	sense,riboprobes
R8903	T13681	T13680	cc	and,sense
R8904	T13682	T13680	conj	antisense,sense
R8905	T13683	T13684	punct	(,Roche
R8906	T13684	T13679	parataxis	Roche,riboprobes
R8907	T13685	T13684	punct	),Roche
R8908	T13686	T13659	punct	.,used
R8909	T13688	T13689	det	The,probes
R891	T1725	T1727	nsubj	Catenin,binds
R8910	T13689	T13691	nsubjpass	probes,obtained
R8911	T13690	T13689	amod	respective,probes
R8912	T13692	T13691	auxpass	were,obtained
R8913	T13693	T13691	prep	by,obtained
R8914	T13694	T13695	nmod	XhoI,digestions
R8915	T13695	T13693	pobj	digestions,by
R8916	T13696	T13694	cc	and,XhoI
R8917	T13697	T13694	conj	BamH1,XhoI
R8918	T13698	T13691	punct	.,obtained
R8919	T13700	T13701	advmod	In,situ
R892	T1726	T1725	punct	-,Catenin
R8920	T13701	T13702	amod	situ,hybridizations
R8921	T13702	T13703	nsubjpass	hybridizations,performed
R8922	T13704	T13703	auxpass	were,performed
R8923	T13705	T13703	prep	on,performed
R8924	T13706	T13707	nummod	10,μm
R8925	T13707	T13709	npadvmod	μm,thick
R8926	T13708	T13707	punct	-,μm
R8927	T13709	T13710	amod	thick,sections
R8928	T13710	T13705	pobj	sections,on
R8929	T13711	T13710	prep	of,sections
R893	T1780	T1779	compound	protein,kinase
R8930	T13712	T13713	compound	E17.5,embryos
R8931	T13713	T13711	pobj	embryos,of
R8932	T13714	T13713	compound	mouse,embryos
R8933	T13715	T13703	punct	.,performed
R8934	T13717	T13718	det	The,sections
R8935	T13718	T13719	nsubjpass	sections,fixed
R8936	T13720	T13719	auxpass	were,fixed
R8937	T13721	T13719	prep	with,fixed
R8938	T13722	T13723	nummod	4,%
R8939	T13723	T13724	compound	%,PFA
R894	T1781	T1779	punct	(,kinase
R8940	T13724	T13721	pobj	PFA,with
R8941	T13725	T13719	prep	for,fixed
R8942	T13726	T13727	nummod	10,min
R8943	T13727	T13725	pobj	min,for
R8944	T13728	T13719	prep	at,fixed
R8945	T13729	T13730	compound	room,temperature
R8946	T13730	T13728	pobj	temperature,at
R8947	T13731	T13719	punct	", ",fixed
R8948	T13732	T13719	conj	prehybridized,fixed
R8949	T13733	T13732	prep	at,prehybridized
R895	T1782	T1779	appos	MAPK,kinase
R8950	T13734	T13735	compound	room,temperature
R8951	T13735	T13733	pobj	temperature,at
R8952	T13736	T13732	prep	for,prehybridized
R8953	T13737	T13738	nummod	4.5,h
R8954	T13738	T13736	pobj	h,for
R8955	T13739	T13732	punct	", ",prehybridized
R8956	T13740	T13732	conj	hybridized,prehybridized
R8957	T13741	T13740	prep	with,hybridized
R8958	T13742	T13743	det	the,probe
R8959	T13743	T13741	pobj	probe,with
R896	T1728	T1727	advmod	also,binds
R8960	T13744	T13745	punct	(,μg
R8961	T13745	T13743	parataxis	μg,probe
R8962	T13746	T13745	nummod	2,μg
R8963	T13747	T13748	punct	/,ml
R8964	T13748	T13745	prep	ml,μg
R8965	T13749	T13745	punct	),μg
R8966	T13750	T13740	prep	at,hybridized
R8967	T13751	T13752	nummod	55,°C
R8968	T13752	T13750	pobj	°C,at
R8969	T13753	T13740	prep	for,hybridized
R897	T1783	T1773	punct	),pathway
R8970	T13754	T13755	quantmod	12,14
R8971	T13755	T13757	nummod	14,h
R8972	T13756	T13755	punct	–,14
R8973	T13757	T13753	pobj	h,for
R8974	T13758	T13740	punct	", ",hybridized
R8975	T13759	T13740	conj	blocked,hybridized
R8976	T13760	T13759	prep	with,blocked
R8977	T13761	T13762	nummod	10,%
R8978	T13762	T13763	compound	%,NGS
R8979	T13763	T13760	pobj	NGS,with
R898	T1784	T1785	punct	[,10
R8980	T13764	T13759	punct	", ",blocked
R8981	T13765	T13759	cc	and,blocked
R8982	T13766	T13759	conj	treated,blocked
R8983	T13767	T13766	prep	with,treated
R8984	T13768	T13769	amod	anti-dig,antibody
R8985	T13769	T13767	pobj	antibody,with
R8986	T13770	T13771	compound	Fab,AP
R8987	T13771	T13769	compound	AP,antibody
R8988	T13772	T13771	punct	-,AP
R8989	T13773	T13774	punct	(,Roche
R899	T1729	T1727	prep	to,binds
R8990	T13774	T13769	parataxis	Roche,antibody
R8991	T13775	T13774	punct	#,Roche
R8992	T13776	T13774	nummod	1093274,Roche
R8993	T13777	T13774	punct	),Roche
R8994	T13778	T13766	prep	at,treated
R8995	T13779	T13780	det	a,dilution
R8996	T13780	T13778	pobj	dilution,at
R8997	T13781	T13782	quantmod	1,"2,500"
R8998	T13782	T13780	nummod	"2,500",dilution
R8999	T13783	T13782	punct	:,"2,500"
R900	T1785	T1727	parataxis	10,binds
R9000	T13784	T13766	prep	for,treated
R9001	T13785	T13786	nummod	3,h
R9002	T13786	T13784	pobj	h,for
R9003	T13787	T13719	punct	.,fixed
R9004	T13789	T13790	det	The,sections
R9005	T13790	T13791	nsubjpass	sections,incubated
R9006	T13792	T13791	auxpass	were,incubated
R9007	T13793	T13791	prep	with,incubated
R9008	T13794	T13793	pobj	NBT,with
R9009	T13795	T13794	cc	and,NBT
R901	T1786	T1785	nummod	9,10
R9010	T13796	T13794	conj	BCIP,NBT
R9011	T13797	T13798	mark	until,detected
R9012	T13798	T13791	advcl	detected,incubated
R9013	T13799	T13800	amod	adequate,signal
R9014	T13800	T13798	nsubjpass	signal,detected
R9015	T13801	T13798	auxpass	was,detected
R9016	T13802	T13791	punct	.,incubated
R9017	T13829	T13828	cc	and,Immunofluorescence
R9018	T13830	T13828	conj	immunohistochemistry,Immunofluorescence
R9019	T13832	T13833	compound	Tissue,samples
R902	T1730	T1731	det	the,protein
R9020	T13833	T13834	nsubjpass	samples,frozen
R9021	T13835	T13833	prep	for,samples
R9022	T13836	T13835	pobj	immunofluorescence,for
R9023	T13837	T13834	auxpass	were,frozen
R9024	T13838	T13834	prep	in,frozen
R9025	T13839	T13838	pobj	OCT,in
R9026	T13840	T13834	cc	and,frozen
R9027	T13841	T13834	conj	sectioned,frozen
R9028	T13842	T13843	nummod	10,μm
R9029	T13843	T13844	npadvmod	μm,thick
R903	T1787	T1785	punct	",",10
R9030	T13844	T13841	advcl	thick,sectioned
R9031	T13845	T13841	prep	on,sectioned
R9032	T13846	T13847	det	a,cryostat
R9033	T13847	T13845	pobj	cryostat,on
R9034	T13848	T13834	punct	.,frozen
R9035	T13850	T13851	nsubjpass	Sections,fixed
R9036	T13852	T13851	auxpass	were,fixed
R9037	T13853	T13851	prep	in,fixed
R9038	T13854	T13855	nummod	4,%
R9039	T13855	T13856	compound	%,paraformaldehyde
R904	T1788	T1785	punct	],10
R9040	T13856	T13853	pobj	paraformaldehyde,in
R9041	T13857	T13851	prep	for,fixed
R9042	T13858	T13859	nummod	10,min
R9043	T13859	T13857	pobj	min,for
R9044	T13860	T13851	prep	at,fixed
R9045	T13861	T13862	compound	room,temperature
R9046	T13862	T13860	pobj	temperature,at
R9047	T13863	T13851	punct	", ",fixed
R9048	T13864	T13851	conj	blocked,fixed
R9049	T13865	T13864	punct	", ",blocked
R905	T1731	T1729	pobj	protein,to
R9050	T13866	T13864	cc	and,blocked
R9051	T13867	T13864	conj	stained,blocked
R9052	T13868	T13867	prep	with,stained
R9053	T13869	T13868	pobj	antibodies,with
R9054	T13870	T13851	punct	.,fixed
R9055	T13872	T13873	compound	Tissue,samples
R9056	T13873	T13874	nsubjpass	samples,fixed
R9057	T13875	T13873	prep	for,samples
R9058	T13876	T13875	pobj	immunohistochemistry,for
R9059	T13877	T13874	auxpass	were,fixed
R906	T1789	T1727	punct	.,binds
R9060	T13878	T13874	prep	in,fixed
R9061	T13879	T13880	nummod	4,%
R9062	T13880	T13881	compound	%,paraformaldehyde
R9063	T13881	T13878	pobj	paraformaldehyde,in
R9064	T13882	T13874	punct	", ",fixed
R9065	T13883	T13874	conj	dehydrated,fixed
R9066	T13884	T13883	punct	", ",dehydrated
R9067	T13885	T13883	cc	and,dehydrated
R9068	T13886	T13883	conj	embedded,dehydrated
R9069	T13887	T13886	prep	in,embedded
R907	T1732	T1731	nmod	class,protein
R9070	T13888	T13887	pobj	paraffin,in
R9071	T13889	T13874	punct	.,fixed
R9072	T13891	T13892	nsubjpass	Samples,sectioned
R9073	T13893	T13892	auxpass	were,sectioned
R9074	T13894	T13892	prep	on,sectioned
R9075	T13895	T13896	det	a,microtome
R9076	T13896	T13894	pobj	microtome,on
R9077	T13897	T13898	punct	(,thick
R9078	T13898	T13892	parataxis	thick,sectioned
R9079	T13899	T13900	nummod	10,μm
R908	T1791	T1792	prep	Through,appear
R9080	T13900	T13898	npadvmod	μm,thick
R9081	T13901	T13898	punct	),thick
R9082	T13902	T13892	cc	and,sectioned
R9083	T13903	T13892	conj	rehydrated,sectioned
R9084	T13904	T13903	prep	prior,rehydrated
R9085	T13905	T13904	pcomp	to,prior
R9086	T13906	T13904	pcomp	staining,prior
R9087	T13907	T13906	prep	with,staining
R9088	T13908	T13907	pobj	antibody,with
R9089	T13909	T13892	punct	.,sectioned
R909	T1793	T1794	det	these,links
R9090	T13911	T13912	nsubjpass	Samples,unmasked
R9091	T13913	T13911	acl	stained,Samples
R9092	T13914	T13913	prep	with,stained
R9093	T13915	T13914	pobj	Snail,with
R9094	T13916	T13915	punct	", ",Snail
R9095	T13917	T13915	conj	pMAPK,Snail
R9096	T13918	T13917	punct	", ",pMAPK
R9097	T13919	T13917	conj	pSmad2,pMAPK
R9098	T13920	T13919	punct	", ",pSmad2
R9099	T13921	T13919	cc	and,pSmad2
R910	T1733	T1732	nummod	III,class
R9100	T13922	T13923	compound	cyclin,D
R9101	T13923	T13919	conj	D,pSmad2
R9102	T13924	T13912	auxpass	were,unmasked
R9103	T13925	T13912	dep	antigen,unmasked
R9104	T13926	T13912	prep	with,unmasked
R9105	T13927	T13928	nummod	10,mM
R9106	T13928	T13929	compound	mM,citrate
R9107	T13929	T13926	pobj	citrate,with
R9108	T13930	T13929	compound	sodium,citrate
R9109	T13931	T13929	punct	(,citrate
R911	T1794	T1791	pobj	links,Through
R9110	T13932	T13929	npadvmod	pH,citrate
R9111	T13933	T13932	nummod	6,pH
R9112	T13934	T13929	punct	),citrate
R9113	T13935	T13912	prep	in,unmasked
R9114	T13936	T13937	det	an,Retriever
R9115	T13937	T13935	pobj	Retriever,in
R9116	T13938	T13937	compound	Antigen,Retriever
R9117	T13939	T13937	nummod	2100,Retriever
R9118	T13940	T13941	punct	(,Laboratories
R9119	T13941	T13937	parataxis	Laboratories,Retriever
R912	T1795	T1792	punct	", ",appear
R9120	T13942	T13941	compound	Pickcell,Laboratories
R9121	T13943	T13941	punct	", ",Laboratories
R9122	T13944	T13941	npadvmod	Leiden,Laboratories
R9123	T13945	T13941	punct	", ",Laboratories
R9124	T13946	T13941	npadvmod	Netherlands,Laboratories
R9125	T13947	T13941	punct	),Laboratories
R9126	T13948	T13912	punct	.,unmasked
R9127	T13950	T13951	det	The,kit
R9128	T13951	T13954	nsubjpass	kit,used
R9129	T13952	T13953	compound	DAB,substrate
R913	T1734	T1731	nmod	Lin,protein
R9130	T13953	T13951	compound	substrate,kit
R9131	T13955	T13956	punct	(,Labs
R9132	T13956	T13951	parataxis	Labs,kit
R9133	T13957	T13956	compound	Vector,Labs
R9134	T13958	T13956	punct	),Labs
R9135	T13959	T13954	auxpass	was,used
R9136	T13960	T13954	prep	according,used
R9137	T13961	T13960	prep	to,according
R9138	T13962	T13963	poss	manufacturer,instructions
R9139	T13963	T13961	pobj	instructions,to
R914	T1796	T1792	nsubj	AJs,appear
R9140	T13964	T13962	case	's,manufacturer
R9141	T13965	T13966	aux	to,develop
R9142	T13966	T13954	advcl	develop,used
R9143	T13967	T13968	det	the,signal
R9144	T13968	T13966	dobj	signal,develop
R9145	T13969	T13954	punct	.,used
R9146	T14008	T14009	compound	RT,PCR
R9147	T14010	T14009	punct	-,PCR
R9148	T14012	T14013	nsubjpass	RNA,extracted
R9149	T14014	T14013	auxpass	was,extracted
R915	T1797	T1792	oprd	able,appear
R9150	T14015	T14013	prep	from,extracted
R9151	T14016	T14015	pobj	keratinocytes,from
R9152	T14017	T14016	cc	or,keratinocytes
R9153	T14018	T14019	compound	skin,tissue
R9154	T14019	T14016	conj	tissue,keratinocytes
R9155	T14020	T14013	prep	with,extracted
R9156	T14021	T14020	pobj	Trizol,with
R9157	T14022	T14023	punct	(,Invitrogen
R9158	T14023	T14021	parataxis	Invitrogen,Trizol
R9159	T14024	T14023	punct	),Invitrogen
R916	T1798	T1799	aux	to,couple
R9160	T14025	T14013	prep	according,extracted
R9161	T14026	T14025	prep	to,according
R9162	T14027	T14028	det	the,manufacturer
R9163	T14028	T14029	poss	manufacturer,protocol
R9164	T14029	T14026	pobj	protocol,to
R9165	T14030	T14028	case	's,manufacturer
R9166	T14031	T14013	punct	.,extracted
R9167	T14033	T14034	nsubjpass	cDNA,generated
R9168	T14035	T14034	auxpass	was,generated
R9169	T14036	T14034	advcl	using,generated
R917	T1735	T1734	punct	-,Lin
R9170	T14037	T14038	compound	oligo,dT
R9171	T14038	T14040	compound	dT,primers
R9172	T14039	T14038	punct	-,dT
R9173	T14040	T14036	dobj	primers,using
R9174	T14041	T14040	cc	and,primers
R9175	T14042	T14043	det	the,kit
R9176	T14043	T14040	conj	kit,primers
R9177	T14044	T14043	nmod	Superscript,kit
R9178	T14045	T14044	nummod	II,Superscript
R9179	T14046	T14047	punct	(,Invitrogen
R918	T1799	T1797	xcomp	couple,able
R9180	T14047	T14043	parataxis	Invitrogen,kit
R9181	T14048	T14047	punct	),Invitrogen
R9182	T14049	T14034	punct	.,generated
R9183	T14051	T14052	det	The,primers
R9184	T14052	T14053	nsubj	primers,were
R9185	T14054	T14052	acl	used,primers
R9186	T14055	T14054	prep	for,used
R9187	T14056	T14055	pobj	PCR,for
R9188	T14057	T14058	compound	Snail,forward
R9189	T14058	T14053	attr	forward,were
R919	T1800	T1799	dobj	adhesion,couple
R9190	T14059	T14058	punct	: ,forward
R9191	T14060	T14061	nummod	5,CAGCTGGCCAGGCTCTCGGT
R9192	T14061	T14058	appos	CAGCTGGCCAGGCTCTCGGT,forward
R9193	T14062	T14060	punct	′,5
R9194	T14063	T14061	punct	-,CAGCTGGCCAGGCTCTCGGT
R9195	T14064	T14061	punct	-,CAGCTGGCCAGGCTCTCGGT
R9196	T14065	T14061	nummod	3,CAGCTGGCCAGGCTCTCGGT
R9197	T14066	T14061	punct	′,CAGCTGGCCAGGCTCTCGGT
R9198	T14067	T14058	punct	;,forward
R9199	T14068	T14069	compound	Snail,reverse
R920	T1736	T1734	nummod	1,Lin
R9200	T14069	T14058	conj	reverse,forward
R9201	T14070	T14069	punct	: ,reverse
R9202	T14071	T14072	nummod	5,GCGAGGGCCTCCGGAGCA
R9203	T14072	T14069	appos	GCGAGGGCCTCCGGAGCA,reverse
R9204	T14073	T14071	punct	′,5
R9205	T14074	T14072	punct	-,GCGAGGGCCTCCGGAGCA
R9206	T14075	T14072	punct	-,GCGAGGGCCTCCGGAGCA
R9207	T14076	T14072	nummod	3,GCGAGGGCCTCCGGAGCA
R9208	T14077	T14072	punct	′,GCGAGGGCCTCCGGAGCA
R9209	T14078	T14069	punct	;,reverse
R921	T1801	T1799	prep	with,couple
R9210	T14079	T14080	compound	GAPDH,forward
R9211	T14080	T14069	conj	forward,reverse
R9212	T14081	T14082	nummod	5,CGTAGACAAAATGGTGAAGGTCGG
R9213	T14082	T14080	appos	CGTAGACAAAATGGTGAAGGTCGG,forward
R9214	T14083	T14081	punct	′,5
R9215	T14084	T14082	punct	-,CGTAGACAAAATGGTGAAGGTCGG
R9216	T14085	T14082	punct	-,CGTAGACAAAATGGTGAAGGTCGG
R9217	T14086	T14082	nummod	3,CGTAGACAAAATGGTGAAGGTCGG
R9218	T14087	T14082	punct	′,CGTAGACAAAATGGTGAAGGTCGG
R9219	T14088	T14080	punct	;,forward
R922	T1802	T1803	amod	cytoskeletal,dynamics
R9220	T14089	T14080	cc	and,forward
R9221	T14090	T14091	compound	GAPDH,reverse
R9222	T14091	T14080	conj	reverse,forward
R9223	T14092	T14091	punct	: ,reverse
R9224	T14093	T14094	nummod	5,AAGCAGTTGGTGGTGCAGGATG
R9225	T14094	T14091	appos	AAGCAGTTGGTGGTGCAGGATG,reverse
R9226	T14095	T14093	punct	′,5
R9227	T14096	T14094	punct	-,AAGCAGTTGGTGGTGCAGGATG
R9228	T14097	T14094	punct	-,AAGCAGTTGGTGGTGCAGGATG
R9229	T14098	T14094	nummod	3,AAGCAGTTGGTGGTGCAGGATG
R923	T1803	T1801	pobj	dynamics,with
R9230	T14099	T14094	punct	′,AAGCAGTTGGTGGTGCAGGATG
R9231	T14100	T14053	punct	.,were
R924	T1737	T1734	punct	", ",Lin
R925	T1804	T1805	advmod	as,as
R926	T1805	T1801	cc	as,with
R927	T1806	T1805	advmod	well,as
R928	T1738	T1734	appos	Isl,Lin
R929	T1807	T1801	conj	with,with
R930	T1808	T1809	amod	nuclear,signaling
R931	T1739	T1738	punct	-,Isl
R932	T1809	T1807	pobj	signaling,with
R933	T1810	T1808	cc	and,nuclear
R934	T1811	T1808	conj	cytoplasmic,nuclear
R935	T1740	T1738	nummod	1,Isl
R936	T1812	T1792	punct	.,appear
R937	T1814	T1815	nsubj	This,provides
R938	T1741	T1734	punct	", ",Lin
R939	T1816	T1817	det	a,framework
R940	T1742	T1734	appos	Mec,Lin
R941	T1817	T1815	dobj	framework,provides
R942	T1818	T1817	prep	for,framework
R943	T1819	T1818	pcomp	conceptualizing,for
R944	T1820	T1821	advmod	why,appear
R945	T1821	T1819	ccomp	appear,conceptualizing
R946	T1822	T1823	compound	E,cadherin
R947	T1743	T1742	punct	-,Mec
R948	T1823	T1825	compound	cadherin,levels
R949	T1824	T1823	punct	-,cadherin
R950	T1825	T1821	nsubj	levels,appear
R951	T1826	T1827	aux	to,impact
R952	T1744	T1742	nummod	3,Mec
R953	T1827	T1821	xcomp	impact,appear
R954	T1828	T1827	prep	upon,impact
R955	T1745	T1734	punct	(,Lin
R956	T1829	T1830	det	a,plethora
R957	T1830	T1828	pobj	plethora,upon
R958	T1831	T1830	prep	of,plethora
R959	T1746	T1734	appos	LIM,Lin
R960	T1832	T1833	amod	developmental,processes
R961	T1833	T1831	pobj	processes,of
R962	T1834	T1835	punct	(,reviewed
R963	T1747	T1731	punct	),protein
R964	T1835	T1815	parataxis	reviewed,provides
R965	T1836	T1835	prep	in,reviewed
R966	T1837	T1836	punct	[,in
R967	T1748	T1731	appos	Ajuba,protein
R968	T1838	T1836	pobj	11,in
R969	T1839	T1835	punct	],reviewed
R970	T1840	T1835	punct	),reviewed
R971	T1749	T1731	punct	(,protein
R972	T1841	T1815	punct	.,provides
R973	T1750	T1751	det	a,member
R974	T1843	T1844	mark	As,probed
R975	T1844	T1846	advcl	probed,intrigued
R976	T1845	T1844	nsubj	we,probed
R977	T1751	T1731	appos	member,protein
R978	T1847	T1848	advmod	more,deeply
R979	T1848	T1844	advmod	deeply,probed
R980	T1752	T1751	prep	of,member
R981	T1849	T1844	prep	into,probed
R982	T1850	T1851	det	the,mechanisms
R983	T1851	T1849	pobj	mechanisms,into
R984	T1753	T1754	det	the,family
R985	T1852	T1851	amod	underlying,mechanisms
R986	T1853	T1851	acl	governing,mechanisms
R987	T1854	T1855	compound	E,cadherin
R988	T1754	T1752	pobj	family,of
R989	T1855	T1857	compound	cadherin,promoter
R990	T1856	T1855	punct	-,cadherin
R991	T1857	T1858	compound	promoter,activity
R992	T1755	T1754	compound	zyxin,family
R993	T1858	T1853	dobj	activity,governing
R994	T1859	T1846	punct	", ",intrigued
R995	T1756	T1754	prep	of,family
R996	T1860	T1846	nsubjpass	we,intrigued
R997	T1861	T1846	auxpass	were,intrigued
R998	T1862	T1846	agent	by,intrigued
R999	T1863	T1864	det	the,proximity

PMC:539061 / 1627-64171 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

PMC:539061 / 1627-64171 JSON TXT 5 Projects