PMC:539061 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T146	0-1	DT	denotes	A
T148	0-77	sentence	denotes	A Signaling Pathway Involving TGF-β2 and Snail in Hair Follicle Morphogenesis
T149	2-11	NN	denotes	Signaling
T147	12-19	NN	denotes	Pathway
T150	20-29	VBG	denotes	Involving
T151	30-33	NN	denotes	TGF
T153	33-34	HYPH	denotes	-
T152	34-36	NN	denotes	β2
T154	37-40	CC	denotes	and
T155	41-46	NN	denotes	Snail
T156	47-49	IN	denotes	in
T157	50-54	NN	denotes	Hair
T158	55-63	NN	denotes	Follicle
T159	64-77	NN	denotes	Morphogenesis
T160	77-78	sentence	denotes
T162	129-131	IN	denotes	In
T161	129-288	sentence	denotes	In a common theme of organogenesis, certain cells within a multipotent epithelial sheet exchange signals with their neighbors and develop into a bud structure.
T164	132-133	DT	denotes	a
T166	134-140	JJ	denotes	common
T165	141-146	NN	denotes	theme
T167	147-149	IN	denotes	of
T168	150-163	NN	denotes	organogenesis
T169	163-165	,	denotes	,
T170	165-172	JJ	denotes	certain
T171	173-178	NNS	denotes	cells
T172	179-185	IN	denotes	within
T173	186-187	DT	denotes	a
T175	188-199	JJ	denotes	multipotent
T176	200-210	JJ	denotes	epithelial
T174	211-216	NN	denotes	sheet
T163	217-225	VB	denotes	exchange
T177	226-233	NNS	denotes	signals
T178	234-238	IN	denotes	with
T179	239-244	PRP$	denotes	their
T180	245-254	NNS	denotes	neighbors
T181	255-258	CC	denotes	and
T182	259-266	VBP	denotes	develop
T183	267-271	IN	denotes	into
T184	272-273	DT	denotes	a
T186	274-277	NN	denotes	bud
T185	278-287	NN	denotes	structure
T187	287-288	.	denotes	.
T188	288-544	sentence	denotes	Using hair bud morphogenesis as a paradigm, we employed mutant mouse models and cultured keratinocytes to dissect the contributions of multiple extracellular cues in orchestrating adhesion dynamics and proliferation to shape the cluster of cells involved.
T189	289-294	VBG	denotes	Using
T191	295-299	NN	denotes	hair
T192	300-303	NN	denotes	bud
T193	304-317	NN	denotes	morphogenesis
T194	318-320	IN	denotes	as
T195	321-322	DT	denotes	a
T196	323-331	NN	denotes	paradigm
T197	331-333	,	denotes	,
T198	333-335	PRP	denotes	we
T190	336-344	VBD	denotes	employed
T199	345-351	NN	denotes	mutant
T200	352-357	NN	denotes	mouse
T201	358-364	NNS	denotes	models
T202	365-368	CC	denotes	and
T203	369-377	VBN	denotes	cultured
T204	378-391	NNS	denotes	keratinocytes
T205	392-394	TO	denotes	to
T206	395-402	VB	denotes	dissect
T207	403-406	DT	denotes	the
T208	407-420	NNS	denotes	contributions
T209	421-423	IN	denotes	of
T210	424-432	JJ	denotes	multiple
T212	433-446	JJ	denotes	extracellular
T211	447-451	NNS	denotes	cues
T213	452-454	IN	denotes	in
T214	455-468	VBG	denotes	orchestrating
T215	469-477	NN	denotes	adhesion
T216	478-486	NNS	denotes	dynamics
T217	487-490	CC	denotes	and
T218	491-504	NN	denotes	proliferation
T219	505-507	TO	denotes	to
T220	508-513	VB	denotes	shape
T221	514-517	DT	denotes	the
T222	518-525	NN	denotes	cluster
T223	526-528	IN	denotes	of
T224	529-534	NNS	denotes	cells
T225	535-543	VBN	denotes	involved
T226	543-544	.	denotes	.
T227	544-745	sentence	denotes	We found that transforming growth factor β2 signaling is necessary to transiently induce the transcription factor Snail and activate the Ras-mitogen-activated protein kinase (MAPK) pathway in the bud.
T228	545-547	PRP	denotes	We
T229	548-553	VBD	denotes	found
T230	554-558	IN	denotes	that
T232	559-571	VBG	denotes	transforming
T233	572-578	NN	denotes	growth
T235	579-585	NN	denotes	factor
T234	586-588	NN	denotes	β2
T236	589-598	NN	denotes	signaling
T231	599-601	VBZ	denotes	is
T237	602-611	JJ	denotes	necessary
T238	612-614	TO	denotes	to
T240	615-626	RB	denotes	transiently
T239	627-633	VB	denotes	induce
T241	634-637	DT	denotes	the
T243	638-651	NN	denotes	transcription
T244	652-658	NN	denotes	factor
T242	659-664	NN	denotes	Snail
T245	665-668	CC	denotes	and
T246	669-677	VB	denotes	activate
T247	678-681	DT	denotes	the
T249	682-685	NN	denotes	Ras
T251	685-686	HYPH	denotes	-
T252	686-693	NN	denotes	mitogen
T254	693-694	HYPH	denotes	-
T253	694-703	VBN	denotes	activated
T255	704-711	NN	denotes	protein
T250	712-718	NN	denotes	kinase
T256	719-720	-LRB-	denotes	(
T257	720-724	NN	denotes	MAPK
T258	724-725	-RRB-	denotes	)
T248	726-733	NN	denotes	pathway
T259	734-736	IN	denotes	in
T260	737-740	DT	denotes	the
T261	741-744	NN	denotes	bud
T262	744-745	.	denotes	.
T263	745-854	sentence	denotes	In the epidermis, Snail misexpression leads to hyperproliferation and a reduction in intercellular adhesion.
T264	746-748	IN	denotes	In
T266	749-752	DT	denotes	the
T267	753-762	NN	denotes	epidermis
T268	762-764	,	denotes	,
T269	764-769	NN	denotes	Snail
T270	770-783	NN	denotes	misexpression
T265	784-789	VBZ	denotes	leads
T271	790-792	IN	denotes	to
T272	793-811	NN	denotes	hyperproliferation
T273	812-815	CC	denotes	and
T274	816-817	DT	denotes	a
T275	818-827	NN	denotes	reduction
T276	828-830	IN	denotes	in
T277	831-844	JJ	denotes	intercellular
T278	845-853	NN	denotes	adhesion
T279	853-854	.	denotes	.
T280	854-1068	sentence	denotes	When E-cadherin is transcriptionally down-regulated, associated adhesion proteins with dual functions in signaling are released from cell-cell contacts, a process which we demonstrate leads to Ras-MAPK activation.
T281	855-859	WRB	denotes	When
T283	860-861	NN	denotes	E
T285	861-862	HYPH	denotes	-
T284	862-870	NN	denotes	cadherin
T286	871-873	VBZ	denotes	is
T287	874-891	RB	denotes	transcriptionally
T288	892-896	RB	denotes	down
T289	896-897	HYPH	denotes	-
T282	897-906	VBN	denotes	regulated
T291	906-908	,	denotes	,
T292	908-918	VBN	denotes	associated
T294	919-927	NN	denotes	adhesion
T293	928-936	NN	denotes	proteins
T295	937-941	IN	denotes	with
T296	942-946	JJ	denotes	dual
T297	947-956	NNS	denotes	functions
T298	957-959	IN	denotes	in
T299	960-969	NN	denotes	signaling
T300	970-973	VBP	denotes	are
T290	974-982	VBN	denotes	released
T301	983-987	IN	denotes	from
T302	988-992	NN	denotes	cell
T304	992-993	HYPH	denotes	-
T303	993-997	NN	denotes	cell
T305	998-1006	NNS	denotes	contacts
T306	1006-1008	,	denotes	,
T307	1008-1009	DT	denotes	a
T308	1010-1017	NN	denotes	process
T309	1018-1023	WDT	denotes	which
T311	1024-1026	PRP	denotes	we
T310	1027-1038	VBP	denotes	demonstrate
T312	1039-1044	VBZ	denotes	leads
T313	1045-1047	IN	denotes	to
T314	1048-1051	NN	denotes	Ras
T316	1051-1052	HYPH	denotes	-
T315	1052-1056	NN	denotes	MAPK
T317	1057-1067	NN	denotes	activation
T318	1067-1068	.	denotes	.
T319	1068-1263	sentence	denotes	These studies provide insights into how multipotent cells within a sheet are stimulated to undergo transcriptional changes that result in proliferation, junctional remodeling, and bud formation.
T320	1069-1074	DT	denotes	These
T321	1075-1082	NNS	denotes	studies
T322	1083-1090	VBP	denotes	provide
T323	1091-1099	NNS	denotes	insights
T324	1100-1104	IN	denotes	into
T325	1105-1108	WRB	denotes	how
T327	1109-1120	JJ	denotes	multipotent
T328	1121-1126	NNS	denotes	cells
T329	1127-1133	IN	denotes	within
T330	1134-1135	DT	denotes	a
T331	1136-1141	NN	denotes	sheet
T332	1142-1145	VBP	denotes	are
T326	1146-1156	VBN	denotes	stimulated
T333	1157-1159	TO	denotes	to
T334	1160-1167	VB	denotes	undergo
T335	1168-1183	JJ	denotes	transcriptional
T336	1184-1191	NNS	denotes	changes
T337	1192-1196	WDT	denotes	that
T338	1197-1203	VBP	denotes	result
T339	1204-1206	IN	denotes	in
T340	1207-1220	NN	denotes	proliferation
T341	1220-1222	,	denotes	,
T342	1222-1232	JJ	denotes	junctional
T343	1233-1243	NN	denotes	remodeling
T344	1243-1245	,	denotes	,
T345	1245-1248	CC	denotes	and
T346	1249-1252	NN	denotes	bud
T347	1253-1262	NN	denotes	formation
T348	1262-1263	.	denotes	.
T349	1263-1444	sentence	denotes	This novel signaling pathway further weaves together the web of different morphogens and downstream transcriptional events that guide hair bud formation within the developing skin.
T350	1264-1268	DT	denotes	This
T352	1269-1274	JJ	denotes	novel
T353	1275-1284	NN	denotes	signaling
T351	1285-1292	NN	denotes	pathway
T355	1293-1300	RB	denotes	further
T354	1301-1307	VBZ	denotes	weaves
T356	1308-1316	RP	denotes	together
T357	1317-1320	DT	denotes	the
T358	1321-1324	NN	denotes	web
T359	1325-1327	IN	denotes	of
T360	1328-1337	JJ	denotes	different
T361	1338-1348	NNS	denotes	morphogens
T362	1349-1352	CC	denotes	and
T363	1353-1363	JJ	denotes	downstream
T365	1364-1379	JJ	denotes	transcriptional
T364	1380-1386	NNS	denotes	events
T366	1387-1391	WDT	denotes	that
T367	1392-1397	VBP	denotes	guide
T368	1398-1402	NN	denotes	hair
T369	1403-1406	NN	denotes	bud
T370	1407-1416	NN	denotes	formation
T371	1417-1423	IN	denotes	within
T372	1424-1427	DT	denotes	the
T374	1428-1438	VBG	denotes	developing
T373	1439-1443	NN	denotes	skin
T375	1443-1444	.	denotes	.
T1019	1640-1649	JJ	denotes	Mammalian
T1020	1650-1661	NN	denotes	development
T1021	1662-1670	VBZ	denotes	involves
T1022	1671-1674	DT	denotes	the
T1023	1675-1688	NN	denotes	morphogenesis
T1024	1689-1691	IN	denotes	of
T1025	1692-1699	JJ	denotes	complex
T1027	1700-1705	CD	denotes	three
T1029	1705-1706	HYPH	denotes	-
T1028	1706-1717	JJ	denotes	dimensional
T1026	1718-1728	NNS	denotes	structures
T1030	1729-1733	IN	denotes	from
T1031	1734-1743	RB	denotes	seemingly
T1032	1744-1751	JJ	denotes	uniform
T1033	1752-1758	NNS	denotes	sheets
T1034	1759-1761	CC	denotes	or
T1035	1762-1768	NNS	denotes	masses
T1036	1769-1771	IN	denotes	of
T1037	1772-1777	NNS	denotes	cells
T1038	1777-1778	.	denotes	.
T1039	1778-1916	sentence	denotes	A simple bud-like structure initiates the formation of many organs, including lungs, spinal cord, mammary glands, and hair follicles [1].
T1040	1779-1780	DT	denotes	A
T1042	1781-1787	JJ	denotes	simple
T1043	1788-1791	NN	denotes	bud
T1045	1791-1792	HYPH	denotes	-
T1044	1792-1796	JJ	denotes	like
T1041	1797-1806	NN	denotes	structure
T1046	1807-1816	VBZ	denotes	initiates
T1047	1817-1820	DT	denotes	the
T1048	1821-1830	NN	denotes	formation
T1049	1831-1833	IN	denotes	of
T1050	1834-1838	JJ	denotes	many
T1051	1839-1845	NNS	denotes	organs
T1052	1845-1847	,	denotes	,
T1053	1847-1856	VBG	denotes	including
T1054	1857-1862	NNS	denotes	lungs
T1055	1862-1864	,	denotes	,
T1056	1864-1870	JJ	denotes	spinal
T1057	1871-1875	NN	denotes	cord
T1058	1875-1877	,	denotes	,
T1059	1877-1884	JJ	denotes	mammary
T1060	1885-1891	NNS	denotes	glands
T1061	1891-1893	,	denotes	,
T1062	1893-1896	CC	denotes	and
T1063	1897-1901	NN	denotes	hair
T1064	1902-1911	NNS	denotes	follicles
T1065	1912-1913	-LRB-	denotes	[
T1066	1913-1914	CD	denotes	1
T1067	1914-1915	-RRB-	denotes	]
T1068	1915-1916	.	denotes	.
T1069	1916-2094	sentence	denotes	The multipotent, adhering epithelial cells are typically attached to an underlying basal lamina that polarizes the epithelial sheet and separates it from surrounding mesenchyme.
T1070	1917-1920	DT	denotes	The
T1072	1921-1932	JJ	denotes	multipotent
T1073	1932-1934	,	denotes	,
T1074	1934-1942	VBG	denotes	adhering
T1075	1943-1953	JJ	denotes	epithelial
T1071	1954-1959	NNS	denotes	cells
T1077	1960-1963	VBP	denotes	are
T1078	1964-1973	RB	denotes	typically
T1076	1974-1982	VBN	denotes	attached
T1079	1983-1985	IN	denotes	to
T1080	1986-1988	DT	denotes	an
T1082	1989-1999	VBG	denotes	underlying
T1083	2000-2005	JJ	denotes	basal
T1081	2006-2012	NN	denotes	lamina
T1084	2013-2017	WDT	denotes	that
T1085	2018-2027	VBZ	denotes	polarizes
T1086	2028-2031	DT	denotes	the
T1088	2032-2042	JJ	denotes	epithelial
T1087	2043-2048	NN	denotes	sheet
T1089	2049-2052	CC	denotes	and
T1090	2053-2062	VBZ	denotes	separates
T1091	2063-2065	PRP	denotes	it
T1092	2066-2070	IN	denotes	from
T1093	2071-2082	VBG	denotes	surrounding
T1094	2083-2093	NN	denotes	mesenchyme
T1095	2093-2094	.	denotes	.
T1096	2094-2274	sentence	denotes	Budding morphogenesis is guided by a reciprocal exchange of signals between epithelium and mesenchyme to specify the identity of the organ that will form and to govern its growth.
T1097	2095-2102	VBG	denotes	Budding
T1098	2103-2116	NN	denotes	morphogenesis
T1100	2117-2119	VBZ	denotes	is
T1099	2120-2126	VBN	denotes	guided
T1101	2127-2129	IN	denotes	by
T1102	2130-2131	DT	denotes	a
T1104	2132-2142	JJ	denotes	reciprocal
T1103	2143-2151	NN	denotes	exchange
T1105	2152-2154	IN	denotes	of
T1106	2155-2162	NNS	denotes	signals
T1107	2163-2170	IN	denotes	between
T1108	2171-2181	NN	denotes	epithelium
T1109	2182-2185	CC	denotes	and
T1110	2186-2196	NN	denotes	mesenchyme
T1111	2197-2199	TO	denotes	to
T1112	2200-2207	VB	denotes	specify
T1113	2208-2211	DT	denotes	the
T1114	2212-2220	NN	denotes	identity
T1115	2221-2223	IN	denotes	of
T1116	2224-2227	DT	denotes	the
T1117	2228-2233	NN	denotes	organ
T1118	2234-2238	WDT	denotes	that
T1120	2239-2243	MD	denotes	will
T1119	2244-2248	VB	denotes	form
T1121	2249-2252	CC	denotes	and
T1122	2253-2255	TO	denotes	to
T1123	2256-2262	VB	denotes	govern
T1124	2263-2266	PRP$	denotes	its
T1125	2267-2273	NN	denotes	growth
T1126	2273-2274	.	denotes	.
T1127	2274-2450	sentence	denotes	At the helm of these molecular communication pathways are Wnts, bone morphogenic proteins (BMPs), transforming growth factor βs (TGF-βs), and fibroblast growth factors (FGFs).
T1128	2275-2277	IN	denotes	At
T1130	2278-2281	DT	denotes	the
T1131	2282-2286	NN	denotes	helm
T1132	2287-2289	IN	denotes	of
T1133	2290-2295	DT	denotes	these
T1135	2296-2305	JJ	denotes	molecular
T1136	2306-2319	NN	denotes	communication
T1134	2320-2328	NNS	denotes	pathways
T1129	2329-2332	VBP	denotes	are
T1137	2333-2337	NNS	denotes	Wnts
T1138	2337-2339	,	denotes	,
T1139	2339-2343	NN	denotes	bone
T1141	2344-2355	JJ	denotes	morphogenic
T1140	2356-2364	NN	denotes	proteins
T1142	2365-2366	-LRB-	denotes	(
T1143	2366-2370	NNS	denotes	BMPs
T1144	2370-2371	-RRB-	denotes	)
T1145	2371-2373	,	denotes	,
T1146	2373-2385	VBG	denotes	transforming
T1148	2386-2392	NN	denotes	growth
T1149	2393-2399	NN	denotes	factor
T1147	2400-2402	NNS	denotes	βs
T1150	2403-2404	-LRB-	denotes	(
T1151	2404-2407	NN	denotes	TGF
T1153	2407-2408	HYPH	denotes	-
T1152	2408-2410	NNS	denotes	βs
T1154	2410-2411	-RRB-	denotes	)
T1155	2411-2413	,	denotes	,
T1156	2413-2416	CC	denotes	and
T1157	2417-2427	NN	denotes	fibroblast
T1159	2428-2434	NN	denotes	growth
T1158	2435-2442	NNS	denotes	factors
T1160	2443-2444	-LRB-	denotes	(
T1161	2444-2448	NNS	denotes	FGFs
T1162	2448-2449	-RRB-	denotes	)
T1163	2449-2450	.	denotes	.
T1164	2450-2674	sentence	denotes	Through activation of cell surface transmembrane receptors, these external signaling molecules trigger distinct cascades of intracellular events that culminate in changes in gene expression, growth, and differentiation [2].
T1165	2451-2458	IN	denotes	Through
T1167	2459-2469	NN	denotes	activation
T1168	2470-2472	IN	denotes	of
T1169	2473-2477	NN	denotes	cell
T1170	2478-2485	NN	denotes	surface
T1172	2486-2499	NN	denotes	transmembrane
T1171	2500-2509	NNS	denotes	receptors
T1173	2509-2511	,	denotes	,
T1174	2511-2516	DT	denotes	these
T1176	2517-2525	JJ	denotes	external
T1177	2526-2535	NN	denotes	signaling
T1175	2536-2545	NNS	denotes	molecules
T1166	2546-2553	VBP	denotes	trigger
T1178	2554-2562	JJ	denotes	distinct
T1179	2563-2571	NNS	denotes	cascades
T1180	2572-2574	IN	denotes	of
T1181	2575-2588	JJ	denotes	intracellular
T1182	2589-2595	NNS	denotes	events
T1183	2596-2600	WDT	denotes	that
T1184	2601-2610	VBP	denotes	culminate
T1185	2611-2613	IN	denotes	in
T1186	2614-2621	NNS	denotes	changes
T1187	2622-2624	IN	denotes	in
T1188	2625-2629	NN	denotes	gene
T1189	2630-2640	NN	denotes	expression
T1190	2640-2642	,	denotes	,
T1191	2642-2648	NN	denotes	growth
T1192	2648-2650	,	denotes	,
T1193	2650-2653	CC	denotes	and
T1194	2654-2669	NN	denotes	differentiation
T1195	2670-2671	-LRB-	denotes	[
T1196	2671-2672	CD	denotes	2
T1197	2672-2673	-RRB-	denotes	]
T1198	2673-2674	.	denotes	.
T1199	2674-2847	sentence	denotes	How this constellation of signals collaborates in tailoring each budding process so that it executes a distinct morphogenetic program has yet to be comprehensively defined.
T1200	2675-2678	WRB	denotes	How
T1202	2679-2683	DT	denotes	this
T1203	2684-2697	NN	denotes	constellation
T1204	2698-2700	IN	denotes	of
T1205	2701-2708	NNS	denotes	signals
T1201	2709-2721	VBZ	denotes	collaborates
T1207	2722-2724	IN	denotes	in
T1208	2725-2734	VBG	denotes	tailoring
T1209	2735-2739	DT	denotes	each
T1211	2740-2747	NN	denotes	budding
T1210	2748-2755	NN	denotes	process
T1212	2756-2758	IN	denotes	so
T1214	2759-2763	IN	denotes	that
T1215	2764-2766	PRP	denotes	it
T1213	2767-2775	VBZ	denotes	executes
T1216	2776-2777	DT	denotes	a
T1218	2778-2786	JJ	denotes	distinct
T1219	2787-2800	JJ	denotes	morphogenetic
T1217	2801-2808	NN	denotes	program
T1206	2809-2812	VBZ	denotes	has
T1220	2813-2816	RB	denotes	yet
T1222	2817-2819	TO	denotes	to
T1223	2820-2822	VB	denotes	be
T1224	2823-2838	RB	denotes	comprehensively
T1221	2839-2846	VBN	denotes	defined
T1225	2846-2847	.	denotes	.
T1226	2847-3073	sentence	denotes	However, the process appears to be patterned at the initial stages of bud formation, since the relative importance of these pathways and their downstream effectors differ as buds begin to develop and cell fates are specified.
T1227	2848-2855	RB	denotes	However
T1229	2855-2857	,	denotes	,
T1230	2857-2860	DT	denotes	the
T1231	2861-2868	NN	denotes	process
T1228	2869-2876	VBZ	denotes	appears
T1232	2877-2879	TO	denotes	to
T1234	2880-2882	VB	denotes	be
T1233	2883-2892	VBN	denotes	patterned
T1235	2893-2895	IN	denotes	at
T1236	2896-2899	DT	denotes	the
T1238	2900-2907	JJ	denotes	initial
T1237	2908-2914	NNS	denotes	stages
T1239	2915-2917	IN	denotes	of
T1240	2918-2921	NN	denotes	bud
T1241	2922-2931	NN	denotes	formation
T1242	2931-2933	,	denotes	,
T1243	2933-2938	IN	denotes	since
T1245	2939-2942	DT	denotes	the
T1247	2943-2951	JJ	denotes	relative
T1246	2952-2962	NN	denotes	importance
T1248	2963-2965	IN	denotes	of
T1249	2966-2971	DT	denotes	these
T1250	2972-2980	NNS	denotes	pathways
T1251	2981-2984	CC	denotes	and
T1252	2985-2990	PRP$	denotes	their
T1254	2991-3001	JJ	denotes	downstream
T1253	3002-3011	NNS	denotes	effectors
T1244	3012-3018	VBP	denotes	differ
T1255	3019-3021	IN	denotes	as
T1257	3022-3026	NNS	denotes	buds
T1256	3027-3032	VBP	denotes	begin
T1258	3033-3035	TO	denotes	to
T1259	3036-3043	VB	denotes	develop
T1260	3044-3047	CC	denotes	and
T1261	3048-3052	NN	denotes	cell
T1262	3053-3058	NNS	denotes	fates
T1264	3059-3062	VBP	denotes	are
T1263	3063-3072	VBN	denotes	specified
T1265	3072-3073	.	denotes	.
T1266	3073-3205	sentence	denotes	The development of a bud requires a number of coordinated changes in the behavior of the targeted cells within an epithelial sheet.
T1267	3074-3077	DT	denotes	The
T1268	3078-3089	NN	denotes	development
T1270	3090-3092	IN	denotes	of
T1271	3093-3094	DT	denotes	a
T1272	3095-3098	NN	denotes	bud
T1269	3099-3107	VBZ	denotes	requires
T1273	3108-3109	DT	denotes	a
T1274	3110-3116	NN	denotes	number
T1275	3117-3119	IN	denotes	of
T1276	3120-3131	VBN	denotes	coordinated
T1277	3132-3139	NNS	denotes	changes
T1278	3140-3142	IN	denotes	in
T1279	3143-3146	DT	denotes	the
T1280	3147-3155	NN	denotes	behavior
T1281	3156-3158	IN	denotes	of
T1282	3159-3162	DT	denotes	the
T1284	3163-3171	VBN	denotes	targeted
T1283	3172-3177	NNS	denotes	cells
T1285	3178-3184	IN	denotes	within
T1286	3185-3187	DT	denotes	an
T1288	3188-3198	JJ	denotes	epithelial
T1287	3199-3204	NN	denotes	sheet
T1289	3204-3205	.	denotes	.
T1290	3205-3389	sentence	denotes	The process must be accompanied by alterations in the proliferation, polarity, shape, and adhesiveness of selected cells, as well as by modifications in their underlying basal lamina.
T1291	3206-3209	DT	denotes	The
T1292	3210-3217	NN	denotes	process
T1294	3218-3222	MD	denotes	must
T1295	3223-3225	VB	denotes	be
T1293	3226-3237	VBN	denotes	accompanied
T1296	3238-3240	IN	denotes	by
T1297	3241-3252	NNS	denotes	alterations
T1298	3253-3255	IN	denotes	in
T1299	3256-3259	DT	denotes	the
T1300	3260-3273	NN	denotes	proliferation
T1301	3273-3275	,	denotes	,
T1302	3275-3283	NN	denotes	polarity
T1303	3283-3285	,	denotes	,
T1304	3285-3290	NN	denotes	shape
T1305	3290-3292	,	denotes	,
T1306	3292-3295	CC	denotes	and
T1307	3296-3308	NN	denotes	adhesiveness
T1308	3309-3311	IN	denotes	of
T1309	3312-3320	VBN	denotes	selected
T1310	3321-3326	NNS	denotes	cells
T1311	3326-3328	,	denotes	,
T1312	3328-3330	RB	denotes	as
T1314	3331-3335	RB	denotes	well
T1313	3336-3338	IN	denotes	as
T1315	3339-3341	IN	denotes	by
T1316	3342-3355	NNS	denotes	modifications
T1317	3356-3358	IN	denotes	in
T1318	3359-3364	PRP$	denotes	their
T1320	3365-3375	VBG	denotes	underlying
T1321	3376-3381	JJ	denotes	basal
T1319	3382-3388	NN	denotes	lamina
T1322	3388-3389	.	denotes	.
T1323	3389-3617	sentence	denotes	Thus, extracellular epithelial-mesenchymal crosstalk must be intricately orchestrated to couple the determination of distinct cell fates with the contemporaneous remodeling of the physical and structural properties of the cell.
T1324	3390-3394	RB	denotes	Thus
T1326	3394-3396	,	denotes	,
T1327	3396-3409	JJ	denotes	extracellular
T1329	3410-3420	JJ	denotes	epithelial
T1331	3420-3421	HYPH	denotes	-
T1330	3421-3432	JJ	denotes	mesenchymal
T1328	3433-3442	NN	denotes	crosstalk
T1332	3443-3447	MD	denotes	must
T1333	3448-3450	VB	denotes	be
T1334	3451-3462	RB	denotes	intricately
T1325	3463-3475	VBN	denotes	orchestrated
T1335	3476-3478	TO	denotes	to
T1336	3479-3485	VB	denotes	couple
T1337	3486-3489	DT	denotes	the
T1338	3490-3503	NN	denotes	determination
T1339	3504-3506	IN	denotes	of
T1340	3507-3515	JJ	denotes	distinct
T1342	3516-3520	NN	denotes	cell
T1341	3521-3526	NNS	denotes	fates
T1343	3527-3531	IN	denotes	with
T1344	3532-3535	DT	denotes	the
T1346	3536-3551	JJ	denotes	contemporaneous
T1345	3552-3562	NN	denotes	remodeling
T1347	3563-3565	IN	denotes	of
T1348	3566-3569	DT	denotes	the
T1350	3570-3578	JJ	denotes	physical
T1351	3579-3582	CC	denotes	and
T1352	3583-3593	JJ	denotes	structural
T1349	3594-3604	NNS	denotes	properties
T1353	3605-3607	IN	denotes	of
T1354	3608-3611	DT	denotes	the
T1355	3612-3616	NN	denotes	cell
T1356	3616-3617	.	denotes	.
T1357	3617-3733	sentence	denotes	Among the few dispensable organs, hair follicles offer an excellent model system to study epithelial bud formation.
T1358	3618-3623	IN	denotes	Among
T1360	3624-3627	DT	denotes	the
T1362	3628-3631	JJ	denotes	few
T1363	3632-3643	JJ	denotes	dispensable
T1361	3644-3650	NNS	denotes	organs
T1364	3650-3652	,	denotes	,
T1365	3652-3656	NN	denotes	hair
T1366	3657-3666	NNS	denotes	follicles
T1359	3667-3672	VBP	denotes	offer
T1367	3673-3675	DT	denotes	an
T1369	3676-3685	JJ	denotes	excellent
T1370	3686-3691	NN	denotes	model
T1368	3692-3698	NN	denotes	system
T1371	3699-3701	TO	denotes	to
T1372	3702-3707	VB	denotes	study
T1373	3708-3718	JJ	denotes	epithelial
T1374	3719-3722	NN	denotes	bud
T1375	3723-3732	NN	denotes	formation
T1376	3732-3733	.	denotes	.
T1377	3733-3817	sentence	denotes	Mammalian skin epithelium begins as a single sheet of multipotent ectodermal cells.
T1378	3734-3743	JJ	denotes	Mammalian
T1380	3744-3748	NN	denotes	skin
T1379	3749-3759	NN	denotes	epithelium
T1381	3760-3766	VBZ	denotes	begins
T1382	3767-3769	IN	denotes	as
T1383	3770-3771	DT	denotes	a
T1385	3772-3778	JJ	denotes	single
T1384	3779-3784	NN	denotes	sheet
T1386	3785-3787	IN	denotes	of
T1387	3788-3799	JJ	denotes	multipotent
T1389	3800-3810	JJ	denotes	ectodermal
T1388	3811-3816	NNS	denotes	cells
T1390	3816-3817	.	denotes	.
T1391	3817-4004	sentence	denotes	During development, specialized mesenchymal cells populate the skin in a spatially defined pattern to initiate the complex epithelial-mesenchymal crosstalk that will specify the bud [3].
T1392	3818-3824	IN	denotes	During
T1394	3825-3836	NN	denotes	development
T1395	3836-3838	,	denotes	,
T1396	3838-3849	JJ	denotes	specialized
T1398	3850-3861	JJ	denotes	mesenchymal
T1397	3862-3867	NNS	denotes	cells
T1393	3868-3876	VBP	denotes	populate
T1399	3877-3880	DT	denotes	the
T1400	3881-3885	NN	denotes	skin
T1401	3886-3888	IN	denotes	in
T1402	3889-3890	DT	denotes	a
T1404	3891-3900	RB	denotes	spatially
T1405	3901-3908	VBN	denotes	defined
T1403	3909-3916	NN	denotes	pattern
T1406	3917-3919	TO	denotes	to
T1407	3920-3928	VB	denotes	initiate
T1408	3929-3932	DT	denotes	the
T1410	3933-3940	JJ	denotes	complex
T1411	3941-3951	JJ	denotes	epithelial
T1413	3951-3952	HYPH	denotes	-
T1412	3952-3963	JJ	denotes	mesenchymal
T1409	3964-3973	NN	denotes	crosstalk
T1414	3974-3978	WDT	denotes	that
T1416	3979-3983	MD	denotes	will
T1415	3984-3991	VB	denotes	specify
T1417	3992-3995	DT	denotes	the
T1418	3996-3999	NN	denotes	bud
T1419	4000-4001	-LRB-	denotes	[
T1420	4001-4002	CD	denotes	3
T1421	4002-4003	-RRB-	denotes	]
T1422	4003-4004	.	denotes	.
T1423	4004-4225	sentence	denotes	Once committed, a small cluster of epithelial cells, the placode, instructs a group of underlying mesenchymal cells to condense and form the nascent dermal papilla, which will be a permanent fixture of the hair follicle.
T1424	4005-4009	IN	denotes	Once
T1425	4010-4019	VBN	denotes	committed
T1427	4019-4021	,	denotes	,
T1428	4021-4022	DT	denotes	a
T1430	4023-4028	JJ	denotes	small
T1429	4029-4036	NN	denotes	cluster
T1431	4037-4039	IN	denotes	of
T1432	4040-4050	JJ	denotes	epithelial
T1433	4051-4056	NNS	denotes	cells
T1434	4056-4058	,	denotes	,
T1435	4058-4061	DT	denotes	the
T1436	4062-4069	NN	denotes	placode
T1437	4069-4071	,	denotes	,
T1426	4071-4080	VBZ	denotes	instructs
T1438	4081-4082	DT	denotes	a
T1439	4083-4088	NN	denotes	group
T1441	4089-4091	IN	denotes	of
T1442	4092-4102	VBG	denotes	underlying
T1444	4103-4114	JJ	denotes	mesenchymal
T1443	4115-4120	NNS	denotes	cells
T1445	4121-4123	TO	denotes	to
T1440	4124-4132	VB	denotes	condense
T1446	4133-4136	CC	denotes	and
T1447	4137-4141	VB	denotes	form
T1448	4142-4145	DT	denotes	the
T1450	4146-4153	JJ	denotes	nascent
T1451	4154-4160	JJ	denotes	dermal
T1449	4161-4168	NN	denotes	papilla
T1452	4168-4170	,	denotes	,
T1453	4170-4175	WDT	denotes	which
T1455	4176-4180	MD	denotes	will
T1454	4181-4183	VB	denotes	be
T1456	4184-4185	DT	denotes	a
T1458	4186-4195	JJ	denotes	permanent
T1457	4196-4203	NN	denotes	fixture
T1459	4204-4206	IN	denotes	of
T1460	4207-4210	DT	denotes	the
T1462	4211-4215	NN	denotes	hair
T1461	4216-4224	NN	denotes	follicle
T1463	4224-4225	.	denotes	.
T1464	4225-4462	sentence	denotes	Subsequent exchanges between the placode and nascent dermal papilla result in further growth of the follicle into the underlying dermis, or down-growth, and eventual differentiation into the six concentric layers of the mature follicle.
T1465	4226-4236	JJ	denotes	Subsequent
T1466	4237-4246	NNS	denotes	exchanges
T1468	4247-4254	IN	denotes	between
T1469	4255-4258	DT	denotes	the
T1470	4259-4266	NN	denotes	placode
T1471	4267-4270	CC	denotes	and
T1472	4271-4278	JJ	denotes	nascent
T1474	4279-4285	JJ	denotes	dermal
T1473	4286-4293	NN	denotes	papilla
T1467	4294-4300	VBP	denotes	result
T1475	4301-4303	IN	denotes	in
T1476	4304-4311	JJ	denotes	further
T1477	4312-4318	NN	denotes	growth
T1478	4319-4321	IN	denotes	of
T1479	4322-4325	DT	denotes	the
T1480	4326-4334	NN	denotes	follicle
T1481	4335-4339	IN	denotes	into
T1482	4340-4343	DT	denotes	the
T1484	4344-4354	JJ	denotes	underlying
T1483	4355-4361	NN	denotes	dermis
T1485	4361-4363	,	denotes	,
T1486	4363-4365	CC	denotes	or
T1487	4366-4370	JJ	denotes	down
T1489	4370-4371	HYPH	denotes	-
T1488	4371-4377	NN	denotes	growth
T1490	4377-4379	,	denotes	,
T1491	4379-4382	CC	denotes	and
T1492	4383-4391	JJ	denotes	eventual
T1493	4392-4407	NN	denotes	differentiation
T1494	4408-4412	IN	denotes	into
T1495	4413-4416	DT	denotes	the
T1497	4417-4420	CD	denotes	six
T1498	4421-4431	JJ	denotes	concentric
T1496	4432-4438	NNS	denotes	layers
T1499	4439-4441	IN	denotes	of
T1500	4442-4445	DT	denotes	the
T1502	4446-4452	JJ	denotes	mature
T1501	4453-4461	NN	denotes	follicle
T1503	4461-4462	.	denotes	.
T1504	4462-4732	sentence	denotes	Previously, we delineated how two respective epithelial and mesenchymal signals, Wnts and the BMP-inhibitory factor noggin, function in concert to induce lymphoid enhancer factor-1/β-catenin (LEF-1/β-catenin)-mediated gene transcription within the follicle placode [4].
T1505	4463-4473	RB	denotes	Previously
T1507	4473-4475	,	denotes	,
T1508	4475-4477	PRP	denotes	we
T1506	4478-4488	VBD	denotes	delineated
T1509	4489-4492	WRB	denotes	how
T1511	4493-4496	CD	denotes	two
T1513	4497-4507	JJ	denotes	respective
T1514	4508-4518	JJ	denotes	epithelial
T1515	4519-4522	CC	denotes	and
T1516	4523-4534	JJ	denotes	mesenchymal
T1512	4535-4542	NNS	denotes	signals
T1517	4542-4544	,	denotes	,
T1518	4544-4548	NNS	denotes	Wnts
T1519	4549-4552	CC	denotes	and
T1520	4553-4556	DT	denotes	the
T1522	4557-4560	NN	denotes	BMP
T1524	4560-4561	HYPH	denotes	-
T1523	4561-4571	JJ	denotes	inhibitory
T1521	4572-4578	NN	denotes	factor
T1525	4579-4585	NN	denotes	noggin
T1526	4585-4587	,	denotes	,
T1510	4587-4595	VBP	denotes	function
T1527	4596-4598	IN	denotes	in
T1528	4599-4606	NN	denotes	concert
T1529	4607-4609	TO	denotes	to
T1530	4610-4616	VB	denotes	induce
T1531	4617-4625	JJ	denotes	lymphoid
T1533	4626-4634	NN	denotes	enhancer
T1532	4635-4641	NN	denotes	factor
T1535	4641-4642	HYPH	denotes	-
T1536	4642-4643	CD	denotes	1
T1537	4643-4644	HYPH	denotes	/
T1538	4644-4645	NN	denotes	β
T1540	4645-4646	HYPH	denotes	-
T1539	4646-4653	NN	denotes	catenin
T1541	4654-4655	-LRB-	denotes	(
T1542	4655-4658	NN	denotes	LEF
T1543	4658-4659	HYPH	denotes	-
T1544	4659-4660	CD	denotes	1
T1545	4660-4661	HYPH	denotes	/
T1546	4661-4662	NN	denotes	β
T1548	4662-4663	HYPH	denotes	-
T1547	4663-4670	NN	denotes	catenin
T1549	4670-4671	-RRB-	denotes	)
T1550	4671-4672	HYPH	denotes	-
T1534	4672-4680	VBN	denotes	mediated
T1552	4681-4685	NN	denotes	gene
T1551	4686-4699	NN	denotes	transcription
T1553	4700-4706	IN	denotes	within
T1554	4707-4710	DT	denotes	the
T1556	4711-4719	NN	denotes	follicle
T1555	4720-4727	NN	denotes	placode
T1557	4728-4729	-LRB-	denotes	[
T1558	4729-4730	CD	denotes	4
T1559	4730-4731	-RRB-	denotes	]
T1560	4731-4732	.	denotes	.
T1561	4732-4997	sentence	denotes	The downstream changes elicited through convergence of these two early signaling pathways include down-regulation of the gene encoding E-cadherin, the prototypical epithelial cadherin that forms the transmembrane core of intercellular adherens junctions (AJs) [5].
T1562	4733-4736	DT	denotes	The
T1564	4737-4747	JJ	denotes	downstream
T1563	4748-4755	NNS	denotes	changes
T1566	4756-4764	VBN	denotes	elicited
T1567	4765-4772	IN	denotes	through
T1568	4773-4784	NN	denotes	convergence
T1569	4785-4787	IN	denotes	of
T1570	4788-4793	DT	denotes	these
T1572	4794-4797	CD	denotes	two
T1573	4798-4803	JJ	denotes	early
T1574	4804-4813	NN	denotes	signaling
T1571	4814-4822	NNS	denotes	pathways
T1565	4823-4830	VBP	denotes	include
T1575	4831-4835	JJ	denotes	down
T1577	4835-4836	HYPH	denotes	-
T1576	4836-4846	NN	denotes	regulation
T1578	4847-4849	IN	denotes	of
T1579	4850-4853	DT	denotes	the
T1580	4854-4858	NN	denotes	gene
T1581	4859-4867	VBG	denotes	encoding
T1582	4868-4869	NN	denotes	E
T1584	4869-4870	HYPH	denotes	-
T1583	4870-4878	NN	denotes	cadherin
T1585	4878-4880	,	denotes	,
T1586	4880-4883	DT	denotes	the
T1588	4884-4896	JJ	denotes	prototypical
T1589	4897-4907	JJ	denotes	epithelial
T1587	4908-4916	NN	denotes	cadherin
T1590	4917-4921	WDT	denotes	that
T1591	4922-4927	VBZ	denotes	forms
T1592	4928-4931	DT	denotes	the
T1594	4932-4945	JJ	denotes	transmembrane
T1593	4946-4950	NN	denotes	core
T1595	4951-4953	IN	denotes	of
T1596	4954-4967	JJ	denotes	intercellular
T1598	4968-4976	NN	denotes	adherens
T1597	4977-4986	NNS	denotes	junctions
T1599	4987-4988	-LRB-	denotes	(
T1600	4988-4991	NNS	denotes	AJs
T1601	4991-4992	-RRB-	denotes	)
T1602	4993-4994	-LRB-	denotes	[
T1603	4994-4995	CD	denotes	5
T1604	4995-4996	-RRB-	denotes	]
T1605	4996-4997	.	denotes	.
T1606	4997-5262	sentence	denotes	We subsequently showed that when E-cadherin is transgenically elevated in mouse skin, hair follicle morphogenesis is blocked, suggesting that E-cadherin down-regulation is a critical event in governing the adhesion dynamics necessary for budding morphogenesis [4].
T1607	4998-5000	PRP	denotes	We
T1609	5001-5013	RB	denotes	subsequently
T1608	5014-5020	VBD	denotes	showed
T1610	5021-5025	IN	denotes	that
T1612	5026-5030	WRB	denotes	when
T1614	5031-5032	NN	denotes	E
T1616	5032-5033	HYPH	denotes	-
T1615	5033-5041	NN	denotes	cadherin
T1617	5042-5044	VBZ	denotes	is
T1618	5045-5059	RB	denotes	transgenically
T1613	5060-5068	VBN	denotes	elevated
T1619	5069-5071	IN	denotes	in
T1620	5072-5077	NN	denotes	mouse
T1621	5078-5082	NN	denotes	skin
T1622	5082-5084	,	denotes	,
T1623	5084-5088	NN	denotes	hair
T1624	5089-5097	NN	denotes	follicle
T1625	5098-5111	NN	denotes	morphogenesis
T1626	5112-5114	VBZ	denotes	is
T1611	5115-5122	VBN	denotes	blocked
T1627	5122-5124	,	denotes	,
T1628	5124-5134	VBG	denotes	suggesting
T1629	5135-5139	IN	denotes	that
T1631	5140-5141	NN	denotes	E
T1633	5141-5142	HYPH	denotes	-
T1632	5142-5150	NN	denotes	cadherin
T1635	5151-5155	JJ	denotes	down
T1636	5155-5156	HYPH	denotes	-
T1634	5156-5166	NN	denotes	regulation
T1630	5167-5169	VBZ	denotes	is
T1637	5170-5171	DT	denotes	a
T1639	5172-5180	JJ	denotes	critical
T1638	5181-5186	NN	denotes	event
T1640	5187-5189	IN	denotes	in
T1641	5190-5199	VBG	denotes	governing
T1642	5200-5203	DT	denotes	the
T1644	5204-5212	NN	denotes	adhesion
T1643	5213-5221	NNS	denotes	dynamics
T1645	5222-5231	JJ	denotes	necessary
T1646	5232-5235	IN	denotes	for
T1647	5236-5243	NN	denotes	budding
T1648	5244-5257	NN	denotes	morphogenesis
T1649	5258-5259	-LRB-	denotes	[
T1650	5259-5260	CD	denotes	4
T1651	5260-5261	-RRB-	denotes	]
T1652	5261-5262	.	denotes	.
T1653	5262-5310	sentence	denotes	Like LEF-1, E-cadherin also binds to β-catenin.
T1654	5263-5267	IN	denotes	Like
T1656	5268-5271	NN	denotes	LEF
T1657	5271-5272	HYPH	denotes	-
T1658	5272-5273	CD	denotes	1
T1659	5273-5275	,	denotes	,
T1660	5275-5276	NN	denotes	E
T1662	5276-5277	HYPH	denotes	-
T1661	5277-5285	NN	denotes	cadherin
T1663	5286-5290	RB	denotes	also
T1655	5291-5296	VBZ	denotes	binds
T1664	5297-5299	IN	denotes	to
T1665	5300-5301	NN	denotes	β
T1667	5301-5302	HYPH	denotes	-
T1666	5302-5309	NN	denotes	catenin
T1668	5309-5310	.	denotes	.
T1669	5310-5573	sentence	denotes	At sites of cell-cell contact, however, E-cadherin-β-catenin complexes recruit α-catenin, which in turn coordinates the associated actin polymerization dynamics necessary to stabilize nascent AJs and integrate the cytoskeleton across an epithelial sheet [6,7,8].
T1670	5311-5313	IN	denotes	At
T1672	5314-5319	NNS	denotes	sites
T1673	5320-5322	IN	denotes	of
T1674	5323-5327	NN	denotes	cell
T1676	5327-5328	HYPH	denotes	-
T1675	5328-5332	NN	denotes	cell
T1677	5333-5340	NN	denotes	contact
T1678	5340-5342	,	denotes	,
T1679	5342-5349	RB	denotes	however
T1680	5349-5351	,	denotes	,
T1681	5351-5352	NN	denotes	E
T1683	5352-5353	HYPH	denotes	-
T1682	5353-5361	NN	denotes	cadherin
T1685	5361-5362	HYPH	denotes	-
T1686	5362-5363	NN	denotes	β
T1688	5363-5364	HYPH	denotes	-
T1687	5364-5371	NN	denotes	catenin
T1684	5372-5381	NNS	denotes	complexes
T1671	5382-5389	VBP	denotes	recruit
T1689	5390-5391	NN	denotes	α
T1691	5391-5392	HYPH	denotes	-
T1690	5392-5399	NN	denotes	catenin
T1692	5399-5401	,	denotes	,
T1693	5401-5406	WDT	denotes	which
T1695	5407-5409	IN	denotes	in
T1696	5410-5414	NN	denotes	turn
T1694	5415-5426	VBZ	denotes	coordinates
T1697	5427-5430	DT	denotes	the
T1699	5431-5441	VBN	denotes	associated
T1700	5442-5447	NN	denotes	actin
T1701	5448-5462	NN	denotes	polymerization
T1698	5463-5471	NNS	denotes	dynamics
T1702	5472-5481	JJ	denotes	necessary
T1703	5482-5484	TO	denotes	to
T1704	5485-5494	VB	denotes	stabilize
T1705	5495-5502	JJ	denotes	nascent
T1706	5503-5506	NNS	denotes	AJs
T1707	5507-5510	CC	denotes	and
T1708	5511-5520	VB	denotes	integrate
T1709	5521-5524	DT	denotes	the
T1710	5525-5537	NN	denotes	cytoskeleton
T1711	5538-5544	IN	denotes	across
T1712	5545-5547	DT	denotes	an
T1714	5548-5558	JJ	denotes	epithelial
T1713	5559-5564	NN	denotes	sheet
T1715	5565-5566	-LRB-	denotes	[
T1717	5566-5567	CD	denotes	6
T1718	5567-5568	,	denotes	,
T1719	5568-5569	CD	denotes	7
T1720	5569-5570	,	denotes	,
T1716	5570-5571	CD	denotes	8
T1721	5571-5572	-RRB-	denotes	]
T1722	5572-5573	.	denotes	.
T1723	5573-5830	sentence	denotes	α-Catenin also binds to the class III Lin-1, Isl-1, Mec-3 (LIM) protein Ajuba (a member of the zyxin family of proteins), which appears to function dually in both adhesion and in activation of the Ras-mitogen-activated protein kinase (MAPK) pathway [9,10].
T1724	5574-5575	NN	denotes	α
T1726	5575-5576	HYPH	denotes	-
T1725	5576-5583	NN	denotes	Catenin
T1728	5584-5588	RB	denotes	also
T1727	5589-5594	VBZ	denotes	binds
T1729	5595-5597	IN	denotes	to
T1730	5598-5601	DT	denotes	the
T1732	5602-5607	NN	denotes	class
T1733	5608-5611	CD	denotes	III
T1734	5612-5615	NN	denotes	Lin
T1735	5615-5616	HYPH	denotes	-
T1736	5616-5617	CD	denotes	1
T1737	5617-5619	,	denotes	,
T1738	5619-5622	NN	denotes	Isl
T1739	5622-5623	HYPH	denotes	-
T1740	5623-5624	CD	denotes	1
T1741	5624-5626	,	denotes	,
T1742	5626-5629	NN	denotes	Mec
T1743	5629-5630	HYPH	denotes	-
T1744	5630-5631	CD	denotes	3
T1745	5632-5633	-LRB-	denotes	(
T1746	5633-5636	NN	denotes	LIM
T1747	5636-5637	-RRB-	denotes	)
T1731	5638-5645	NN	denotes	protein
T1748	5646-5651	NN	denotes	Ajuba
T1749	5652-5653	-LRB-	denotes	(
T1750	5653-5654	DT	denotes	a
T1751	5655-5661	NN	denotes	member
T1752	5662-5664	IN	denotes	of
T1753	5665-5668	DT	denotes	the
T1755	5669-5674	NN	denotes	zyxin
T1754	5675-5681	NN	denotes	family
T1756	5682-5684	IN	denotes	of
T1757	5685-5693	NN	denotes	proteins
T1758	5693-5694	-RRB-	denotes	)
T1759	5694-5696	,	denotes	,
T1760	5696-5701	WDT	denotes	which
T1761	5702-5709	VBZ	denotes	appears
T1762	5710-5712	TO	denotes	to
T1763	5713-5721	VB	denotes	function
T1764	5722-5728	RB	denotes	dually
T1765	5729-5731	IN	denotes	in
T1766	5732-5736	CC	denotes	both
T1767	5737-5745	NN	denotes	adhesion
T1768	5746-5749	CC	denotes	and
T1769	5750-5752	IN	denotes	in
T1770	5753-5763	NN	denotes	activation
T1771	5764-5766	IN	denotes	of
T1772	5767-5770	DT	denotes	the
T1774	5771-5774	NN	denotes	Ras
T1775	5774-5775	HYPH	denotes	-
T1776	5775-5782	NN	denotes	mitogen
T1778	5782-5783	HYPH	denotes	-
T1777	5783-5792	VBN	denotes	activated
T1780	5793-5800	NN	denotes	protein
T1779	5801-5807	NN	denotes	kinase
T1781	5808-5809	-LRB-	denotes	(
T1782	5809-5813	NN	denotes	MAPK
T1783	5813-5814	-RRB-	denotes	)
T1773	5815-5822	NN	denotes	pathway
T1784	5823-5824	-LRB-	denotes	[
T1786	5824-5825	CD	denotes	9
T1787	5825-5826	,	denotes	,
T1785	5826-5828	CD	denotes	10
T1788	5828-5829	-RRB-	denotes	]
T1789	5829-5830	.	denotes	.
T1790	5830-5964	sentence	denotes	Through these links, AJs appear able to couple adhesion with cytoskeletal dynamics as well as with nuclear and cytoplasmic signaling.
T1791	5831-5838	IN	denotes	Through
T1793	5839-5844	DT	denotes	these
T1794	5845-5850	NNS	denotes	links
T1795	5850-5852	,	denotes	,
T1796	5852-5855	NNS	denotes	AJs
T1792	5856-5862	VBP	denotes	appear
T1797	5863-5867	JJ	denotes	able
T1798	5868-5870	TO	denotes	to
T1799	5871-5877	VB	denotes	couple
T1800	5878-5886	NN	denotes	adhesion
T1801	5887-5891	IN	denotes	with
T1802	5892-5904	JJ	denotes	cytoskeletal
T1803	5905-5913	NNS	denotes	dynamics
T1804	5914-5916	RB	denotes	as
T1806	5917-5921	RB	denotes	well
T1805	5922-5924	IN	denotes	as
T1807	5925-5929	IN	denotes	with
T1808	5930-5937	JJ	denotes	nuclear
T1810	5938-5941	CC	denotes	and
T1811	5942-5953	JJ	denotes	cytoplasmic
T1809	5954-5963	NN	denotes	signaling
T1812	5963-5964	.	denotes	.
T1813	5964-6112	sentence	denotes	This provides a framework for conceptualizing why E-cadherin levels appear to impact upon a plethora of developmental processes (reviewed in [11]).
T1814	5965-5969	DT	denotes	This
T1815	5970-5978	VBZ	denotes	provides
T1816	5979-5980	DT	denotes	a
T1817	5981-5990	NN	denotes	framework
T1818	5991-5994	IN	denotes	for
T1819	5995-6010	VBG	denotes	conceptualizing
T1820	6011-6014	WRB	denotes	why
T1822	6015-6016	NN	denotes	E
T1824	6016-6017	HYPH	denotes	-
T1823	6017-6025	NN	denotes	cadherin
T1825	6026-6032	NNS	denotes	levels
T1821	6033-6039	VBP	denotes	appear
T1826	6040-6042	TO	denotes	to
T1827	6043-6049	VB	denotes	impact
T1828	6050-6054	IN	denotes	upon
T1829	6055-6056	DT	denotes	a
T1830	6057-6065	NN	denotes	plethora
T1831	6066-6068	IN	denotes	of
T1832	6069-6082	JJ	denotes	developmental
T1833	6083-6092	NNS	denotes	processes
T1834	6093-6094	-LRB-	denotes	(
T1835	6094-6102	VBN	denotes	reviewed
T1836	6103-6105	IN	denotes	in
T1837	6106-6107	-LRB-	denotes	[
T1838	6107-6109	CD	denotes	11
T1839	6109-6110	-RRB-	denotes	]
T1840	6110-6111	-RRB-	denotes	)
T1841	6111-6112	.	denotes	.
T1842	6112-6396	sentence	denotes	As we probed more deeply into the underlying mechanisms governing E-cadherin promoter activity, we were intrigued by the close proximity of the LEF-1/β-catenin binding site to a site known to bind the Snail/Slug family of zinc finger transcriptional repressor proteins [12,13,14,15].
T1843	6113-6115	IN	denotes	As
T1845	6116-6118	PRP	denotes	we
T1844	6119-6125	VBD	denotes	probed
T1847	6126-6130	RBR	denotes	more
T1848	6131-6137	RB	denotes	deeply
T1849	6138-6142	IN	denotes	into
T1850	6143-6146	DT	denotes	the
T1852	6147-6157	JJ	denotes	underlying
T1851	6158-6168	NNS	denotes	mechanisms
T1853	6169-6178	VBG	denotes	governing
T1854	6179-6180	NN	denotes	E
T1856	6180-6181	HYPH	denotes	-
T1855	6181-6189	NN	denotes	cadherin
T1857	6190-6198	NN	denotes	promoter
T1858	6199-6207	NN	denotes	activity
T1859	6207-6209	,	denotes	,
T1860	6209-6211	PRP	denotes	we
T1861	6212-6216	VBD	denotes	were
T1846	6217-6226	VBN	denotes	intrigued
T1862	6227-6229	IN	denotes	by
T1863	6230-6233	DT	denotes	the
T1865	6234-6239	JJ	denotes	close
T1864	6240-6249	NN	denotes	proximity
T1866	6250-6252	IN	denotes	of
T1867	6253-6256	DT	denotes	the
T1869	6257-6260	NN	denotes	LEF
T1870	6260-6261	HYPH	denotes	-
T1871	6261-6262	CD	denotes	1
T1872	6262-6263	HYPH	denotes	/
T1873	6263-6264	NN	denotes	β
T1875	6264-6265	HYPH	denotes	-
T1874	6265-6272	NN	denotes	catenin
T1876	6273-6280	NN	denotes	binding
T1868	6281-6285	NN	denotes	site
T1877	6286-6288	IN	denotes	to
T1878	6289-6290	DT	denotes	a
T1879	6291-6295	NN	denotes	site
T1880	6296-6301	VBN	denotes	known
T1881	6302-6304	TO	denotes	to
T1882	6305-6309	VB	denotes	bind
T1883	6310-6313	DT	denotes	the
T1885	6314-6319	NN	denotes	Snail
T1887	6319-6320	HYPH	denotes	/
T1886	6320-6324	NN	denotes	Slug
T1884	6325-6331	NN	denotes	family
T1888	6332-6334	IN	denotes	of
T1889	6335-6339	NN	denotes	zinc
T1890	6340-6346	NN	denotes	finger
T1892	6347-6362	JJ	denotes	transcriptional
T1893	6363-6372	NN	denotes	repressor
T1891	6373-6381	NN	denotes	proteins
T1894	6382-6383	-LRB-	denotes	[
T1896	6383-6385	CD	denotes	12
T1897	6385-6386	,	denotes	,
T1898	6386-6388	CD	denotes	13
T1899	6388-6389	,	denotes	,
T1900	6389-6391	CD	denotes	14
T1901	6391-6392	,	denotes	,
T1895	6392-6394	CD	denotes	15
T1902	6394-6395	-RRB-	denotes	]
T1903	6395-6396	.	denotes	.
T1904	6396-6631	sentence	denotes	Both activity of Snail and down-regulation of E-cadherin play pivotal roles in epithelial to mesenchymal transitions (EMTs), typified by the transformation of polarized, adhering epithelial cells into motile mesenchymal cells [16,17].
T1905	6397-6401	CC	denotes	Both
T1906	6402-6410	NN	denotes	activity
T1908	6411-6413	IN	denotes	of
T1909	6414-6419	NN	denotes	Snail
T1910	6420-6423	CC	denotes	and
T1911	6424-6428	NN	denotes	down
T1913	6428-6429	HYPH	denotes	-
T1912	6429-6439	NN	denotes	regulation
T1914	6440-6442	IN	denotes	of
T1915	6443-6444	NN	denotes	E
T1917	6444-6445	HYPH	denotes	-
T1916	6445-6453	NN	denotes	cadherin
T1907	6454-6458	VBP	denotes	play
T1918	6459-6466	JJ	denotes	pivotal
T1919	6467-6472	NNS	denotes	roles
T1920	6473-6475	IN	denotes	in
T1921	6476-6486	JJ	denotes	epithelial
T1923	6487-6489	IN	denotes	to
T1924	6490-6501	JJ	denotes	mesenchymal
T1922	6502-6513	NNS	denotes	transitions
T1925	6514-6515	-LRB-	denotes	(
T1926	6515-6519	NNS	denotes	EMTs
T1927	6519-6520	-RRB-	denotes	)
T1928	6520-6522	,	denotes	,
T1929	6522-6530	VBN	denotes	typified
T1930	6531-6533	IN	denotes	by
T1931	6534-6537	DT	denotes	the
T1932	6538-6552	NN	denotes	transformation
T1933	6553-6555	IN	denotes	of
T1934	6556-6565	VBN	denotes	polarized
T1936	6565-6567	,	denotes	,
T1937	6567-6575	VBG	denotes	adhering
T1938	6576-6586	JJ	denotes	epithelial
T1935	6587-6592	NNS	denotes	cells
T1939	6593-6597	IN	denotes	into
T1940	6598-6604	JJ	denotes	motile
T1942	6605-6616	JJ	denotes	mesenchymal
T1941	6617-6622	NNS	denotes	cells
T1943	6623-6624	-LRB-	denotes	[
T1945	6624-6626	CD	denotes	16
T1946	6626-6627	,	denotes	,
T1944	6627-6629	CD	denotes	17
T1947	6629-6630	-RRB-	denotes	]
T1948	6630-6631	.	denotes	.
T1949	6631-6817	sentence	denotes	Bud formation differs from an EMT in that E-cadherin activity needs to be down-regulated but not prevented, so that adhesive junctions are remodeled rather than quantitatively impaired.
T1950	6632-6635	NN	denotes	Bud
T1951	6636-6645	NN	denotes	formation
T1952	6646-6653	VBZ	denotes	differs
T1953	6654-6658	IN	denotes	from
T1954	6659-6661	DT	denotes	an
T1955	6662-6665	NN	denotes	EMT
T1956	6666-6668	IN	denotes	in
T1958	6669-6673	DT	denotes	that
T1959	6674-6675	NN	denotes	E
T1961	6675-6676	HYPH	denotes	-
T1960	6676-6684	NN	denotes	cadherin
T1962	6685-6693	NN	denotes	activity
T1957	6694-6699	VBZ	denotes	needs
T1963	6700-6702	TO	denotes	to
T1965	6703-6705	VB	denotes	be
T1966	6706-6710	RB	denotes	down
T1967	6710-6711	HYPH	denotes	-
T1964	6711-6720	VBN	denotes	regulated
T1968	6721-6724	CC	denotes	but
T1969	6725-6728	RB	denotes	not
T1970	6729-6738	VBN	denotes	prevented
T1971	6738-6740	,	denotes	,
T1972	6740-6742	IN	denotes	so
T1974	6743-6747	IN	denotes	that
T1975	6748-6756	JJ	denotes	adhesive
T1976	6757-6766	NNS	denotes	junctions
T1977	6767-6770	VBP	denotes	are
T1973	6771-6780	VBN	denotes	remodeled
T1978	6781-6787	RB	denotes	rather
T1979	6788-6792	IN	denotes	than
T1980	6793-6807	RB	denotes	quantitatively
T1981	6808-6816	VBN	denotes	impaired
T1982	6816-6817	.	denotes	.
T1983	6817-7035	sentence	denotes	Supportive of an underlying ability to fine-tune cadherin expression at the transcriptional level, Snail seems to have an additive effect with LEF-1/β-catenin in negatively modulating E-cadherin promoter activity [4].
T1984	6818-6828	JJ	denotes	Supportive
T1986	6829-6831	IN	denotes	of
T1987	6832-6834	DT	denotes	an
T1989	6835-6845	JJ	denotes	underlying
T1988	6846-6853	NN	denotes	ability
T1990	6854-6856	TO	denotes	to
T1992	6857-6861	RB	denotes	fine
T1993	6861-6862	HYPH	denotes	-
T1991	6862-6866	VB	denotes	tune
T1994	6867-6875	NN	denotes	cadherin
T1995	6876-6886	NN	denotes	expression
T1996	6887-6889	IN	denotes	at
T1997	6890-6893	DT	denotes	the
T1999	6894-6909	JJ	denotes	transcriptional
T1998	6910-6915	NN	denotes	level
T2000	6915-6917	,	denotes	,
T2001	6917-6922	NN	denotes	Snail
T1985	6923-6928	VBZ	denotes	seems
T2002	6929-6931	TO	denotes	to
T2003	6932-6936	VB	denotes	have
T2004	6937-6939	DT	denotes	an
T2006	6940-6948	JJ	denotes	additive
T2005	6949-6955	NN	denotes	effect
T2007	6956-6960	IN	denotes	with
T2008	6961-6964	NN	denotes	LEF
T2009	6964-6965	HYPH	denotes	-
T2010	6965-6966	CD	denotes	1
T2011	6966-6967	HYPH	denotes	/
T2012	6967-6968	NN	denotes	β
T2014	6968-6969	HYPH	denotes	-
T2013	6969-6976	NN	denotes	catenin
T2015	6977-6979	IN	denotes	in
T2016	6980-6990	RB	denotes	negatively
T2017	6991-7001	VBG	denotes	modulating
T2018	7002-7003	NN	denotes	E
T2020	7003-7004	HYPH	denotes	-
T2019	7004-7012	NN	denotes	cadherin
T2021	7013-7021	NN	denotes	promoter
T2022	7022-7030	NN	denotes	activity
T2023	7031-7032	-LRB-	denotes	[
T2024	7032-7033	CD	denotes	4
T2025	7033-7034	-RRB-	denotes	]
T2026	7034-7035	.	denotes	.
T2027	7035-7220	sentence	denotes	In the present study, we discovered that Snail is expressed briefly at an early stage of hair bud formation, when E-cadherin down-regulation and activation of proliferation take place.
T2028	7036-7038	IN	denotes	In
T2030	7039-7042	DT	denotes	the
T2032	7043-7050	JJ	denotes	present
T2031	7051-7056	NN	denotes	study
T2033	7056-7058	,	denotes	,
T2034	7058-7060	PRP	denotes	we
T2029	7061-7071	VBD	denotes	discovered
T2035	7072-7076	IN	denotes	that
T2037	7077-7082	NN	denotes	Snail
T2038	7083-7085	VBZ	denotes	is
T2036	7086-7095	VBN	denotes	expressed
T2039	7096-7103	RB	denotes	briefly
T2040	7104-7106	IN	denotes	at
T2041	7107-7109	DT	denotes	an
T2043	7110-7115	JJ	denotes	early
T2042	7116-7121	NN	denotes	stage
T2044	7122-7124	IN	denotes	of
T2045	7125-7129	NN	denotes	hair
T2046	7130-7133	NN	denotes	bud
T2047	7134-7143	NN	denotes	formation
T2048	7143-7145	,	denotes	,
T2049	7145-7149	WRB	denotes	when
T2051	7150-7151	NN	denotes	E
T2053	7151-7152	HYPH	denotes	-
T2052	7152-7160	NN	denotes	cadherin
T2054	7161-7165	JJ	denotes	down
T2056	7165-7166	HYPH	denotes	-
T2055	7166-7176	NN	denotes	regulation
T2057	7177-7180	CC	denotes	and
T2058	7181-7191	NN	denotes	activation
T2059	7192-7194	IN	denotes	of
T2060	7195-7208	NN	denotes	proliferation
T2050	7209-7213	VBP	denotes	take
T2061	7214-7219	NN	denotes	place
T2062	7219-7220	.	denotes	.
T2063	7220-7323	sentence	denotes	Thereafter, Snail disappears and remains absent during subsequent follicle down-growth and maturation.
T2064	7221-7231	RB	denotes	Thereafter
T2066	7231-7233	,	denotes	,
T2067	7233-7238	NN	denotes	Snail
T2065	7239-7249	VBZ	denotes	disappears
T2068	7250-7253	CC	denotes	and
T2069	7254-7261	VBZ	denotes	remains
T2070	7262-7268	JJ	denotes	absent
T2071	7269-7275	IN	denotes	during
T2072	7276-7286	JJ	denotes	subsequent
T2074	7287-7295	NN	denotes	follicle
T2075	7296-7300	JJ	denotes	down
T2076	7300-7301	HYPH	denotes	-
T2073	7301-7307	NN	denotes	growth
T2077	7308-7311	CC	denotes	and
T2078	7312-7322	NN	denotes	maturation
T2079	7322-7323	.	denotes	.
T2080	7323-7583	sentence	denotes	This exquisite pattern appears to be functionally relevant since altering it in vivo correspondingly affects features associated with hair bud formation, including down-regulation of E-cadherin, increased proliferation, and repressed terminal differentiation.
T2081	7324-7328	DT	denotes	This
T2083	7329-7338	JJ	denotes	exquisite
T2082	7339-7346	NN	denotes	pattern
T2084	7347-7354	VBZ	denotes	appears
T2085	7355-7357	TO	denotes	to
T2086	7358-7360	VB	denotes	be
T2087	7361-7373	RB	denotes	functionally
T2088	7374-7382	JJ	denotes	relevant
T2089	7383-7388	IN	denotes	since
T2091	7389-7397	VBG	denotes	altering
T2092	7398-7400	PRP	denotes	it
T2093	7401-7403	FW	denotes	in
T2094	7404-7408	FW	denotes	vivo
T2095	7409-7424	RB	denotes	correspondingly
T2090	7425-7432	VBZ	denotes	affects
T2096	7433-7441	NNS	denotes	features
T2097	7442-7452	VBN	denotes	associated
T2098	7453-7457	IN	denotes	with
T2099	7458-7462	NN	denotes	hair
T2100	7463-7466	NN	denotes	bud
T2101	7467-7476	NN	denotes	formation
T2102	7476-7478	,	denotes	,
T2103	7478-7487	VBG	denotes	including
T2104	7488-7492	NN	denotes	down
T2106	7492-7493	HYPH	denotes	-
T2105	7493-7503	NN	denotes	regulation
T2107	7504-7506	IN	denotes	of
T2108	7507-7508	NN	denotes	E
T2110	7508-7509	HYPH	denotes	-
T2109	7509-7517	NN	denotes	cadherin
T2111	7517-7519	,	denotes	,
T2112	7519-7528	VBN	denotes	increased
T2113	7529-7542	NN	denotes	proliferation
T2114	7542-7544	,	denotes	,
T2115	7544-7547	CC	denotes	and
T2116	7548-7557	VBN	denotes	repressed
T2118	7558-7566	JJ	denotes	terminal
T2117	7567-7582	NN	denotes	differentiation
T2119	7582-7583	.	denotes	.
T2120	7583-7829	sentence	denotes	Although the temporal spike of Snail in the hair bud is reflected at the mRNA level and seems to follow Wnt signaling and BMP inhibition, LEF-1/β-catenin activation does not appear to induce Snail gene expression in embryonic skin keratinocytes.
T2121	7584-7592	IN	denotes	Although
T2123	7593-7596	DT	denotes	the
T2125	7597-7605	JJ	denotes	temporal
T2124	7606-7611	NN	denotes	spike
T2126	7612-7614	IN	denotes	of
T2127	7615-7620	NN	denotes	Snail
T2128	7621-7623	IN	denotes	in
T2129	7624-7627	DT	denotes	the
T2131	7628-7632	NN	denotes	hair
T2130	7633-7636	NN	denotes	bud
T2132	7637-7639	VBZ	denotes	is
T2122	7640-7649	VBN	denotes	reflected
T2134	7650-7652	IN	denotes	at
T2135	7653-7656	DT	denotes	the
T2137	7657-7661	NN	denotes	mRNA
T2136	7662-7667	NN	denotes	level
T2138	7668-7671	CC	denotes	and
T2139	7672-7677	VBZ	denotes	seems
T2140	7678-7680	TO	denotes	to
T2141	7681-7687	VB	denotes	follow
T2142	7688-7691	NN	denotes	Wnt
T2143	7692-7701	NN	denotes	signaling
T2144	7702-7705	CC	denotes	and
T2145	7706-7709	NN	denotes	BMP
T2146	7710-7720	NN	denotes	inhibition
T2147	7720-7722	,	denotes	,
T2148	7722-7725	NN	denotes	LEF
T2150	7725-7726	HYPH	denotes	-
T2151	7726-7727	CD	denotes	1
T2152	7727-7728	HYPH	denotes	/
T2153	7728-7729	NN	denotes	β
T2155	7729-7730	HYPH	denotes	-
T2154	7730-7737	NN	denotes	catenin
T2149	7738-7748	NN	denotes	activation
T2156	7749-7753	VBZ	denotes	does
T2157	7754-7757	RB	denotes	not
T2133	7758-7764	VB	denotes	appear
T2158	7765-7767	TO	denotes	to
T2159	7768-7774	VB	denotes	induce
T2160	7775-7780	NN	denotes	Snail
T2162	7781-7785	NN	denotes	gene
T2161	7786-7796	NN	denotes	expression
T2163	7797-7799	IN	denotes	in
T2164	7800-7809	JJ	denotes	embryonic
T2166	7810-7814	NN	denotes	skin
T2165	7815-7828	NNS	denotes	keratinocytes
T2167	7828-7829	.	denotes	.
T2168	7829-8088	sentence	denotes	In contrast, we provide in vitro, transgenic (Tg), and gene targeting evidence to show that TGF-β2 and small phenotype– and mothers against decapentaplegic–related protein 2 (SMAD2) signaling are upstream inducers of Snail gene expression in skin epithelium.
T2169	7830-7832	IN	denotes	In
T2171	7833-7841	NN	denotes	contrast
T2172	7841-7843	,	denotes	,
T2173	7843-7845	PRP	denotes	we
T2170	7846-7853	VBP	denotes	provide
T2174	7854-7856	FW	denotes	in
T2175	7857-7862	FW	denotes	vitro
T2177	7862-7864	,	denotes	,
T2178	7864-7874	JJ	denotes	transgenic
T2179	7875-7876	-LRB-	denotes	(
T2180	7876-7878	JJ	denotes	Tg
T2181	7878-7879	-RRB-	denotes	)
T2182	7879-7881	,	denotes	,
T2183	7881-7884	CC	denotes	and
T2184	7885-7889	NN	denotes	gene
T2185	7890-7899	VBG	denotes	targeting
T2176	7900-7908	NN	denotes	evidence
T2186	7909-7911	TO	denotes	to
T2187	7912-7916	VB	denotes	show
T2188	7917-7921	IN	denotes	that
T2190	7922-7925	NN	denotes	TGF
T2192	7925-7926	HYPH	denotes	-
T2191	7926-7928	NN	denotes	β2
T2194	7929-7932	CC	denotes	and
T2195	7933-7938	JJ	denotes	small
T2196	7939-7948	NN	denotes	phenotype
T2198	7948-7949	-LRB-	denotes	–
T2199	7950-7953	CC	denotes	and
T2200	7954-7961	NNS	denotes	mothers
T2201	7962-7969	IN	denotes	against
T2202	7970-7985	NN	denotes	decapentaplegic
T2203	7985-7986	-RRB-	denotes	–
T2197	7986-7993	VBN	denotes	related
T2204	7994-8001	NN	denotes	protein
T2205	8002-8003	CD	denotes	2
T2206	8004-8005	-LRB-	denotes	(
T2207	8005-8010	NN	denotes	SMAD2
T2208	8010-8011	-RRB-	denotes	)
T2193	8012-8021	NN	denotes	signaling
T2189	8022-8025	VBP	denotes	are
T2209	8026-8034	JJ	denotes	upstream
T2210	8035-8043	NNS	denotes	inducers
T2211	8044-8046	IN	denotes	of
T2212	8047-8052	NN	denotes	Snail
T2214	8053-8057	NN	denotes	gene
T2213	8058-8068	NN	denotes	expression
T2215	8069-8071	IN	denotes	in
T2216	8072-8076	NN	denotes	skin
T2217	8077-8087	NN	denotes	epithelium
T2218	8087-8088	.	denotes	.
T2219	8088-8219	sentence	denotes	In the absence of TGF-β2 signaling and Snail gene expression, hair placodes can form, but further follicle down-growth is blocked.
T2220	8089-8091	IN	denotes	In
T2222	8092-8095	DT	denotes	the
T2223	8096-8103	NN	denotes	absence
T2224	8104-8106	IN	denotes	of
T2225	8107-8110	NN	denotes	TGF
T2227	8110-8111	HYPH	denotes	-
T2226	8111-8113	NN	denotes	β2
T2228	8114-8123	NN	denotes	signaling
T2229	8124-8127	CC	denotes	and
T2230	8128-8133	NN	denotes	Snail
T2232	8134-8138	NN	denotes	gene
T2231	8139-8149	NN	denotes	expression
T2233	8149-8151	,	denotes	,
T2234	8151-8155	NN	denotes	hair
T2235	8156-8164	NNS	denotes	placodes
T2236	8165-8168	MD	denotes	can
T2221	8169-8173	VB	denotes	form
T2237	8173-8175	,	denotes	,
T2238	8175-8178	CC	denotes	but
T2239	8179-8186	JJ	denotes	further
T2241	8187-8195	NN	denotes	follicle
T2242	8196-8200	JJ	denotes	down
T2243	8200-8201	HYPH	denotes	-
T2240	8201-8207	NN	denotes	growth
T2245	8208-8210	VBZ	denotes	is
T2244	8211-8218	VBN	denotes	blocked
T2246	8218-8219	.	denotes	.
T2247	8219-8397	sentence	denotes	Our studies point to the view that Snail likely functions downstream of cell fate specification, at a stage where the bud begins to exhibit enhanced proliferation and migration.
T2248	8220-8223	PRP$	denotes	Our
T2249	8224-8231	NNS	denotes	studies
T2250	8232-8237	VBP	denotes	point
T2251	8238-8240	IN	denotes	to
T2252	8241-8244	DT	denotes	the
T2253	8245-8249	NN	denotes	view
T2254	8250-8254	IN	denotes	that
T2256	8255-8260	NN	denotes	Snail
T2257	8261-8267	RB	denotes	likely
T2255	8268-8277	VBZ	denotes	functions
T2258	8278-8288	RB	denotes	downstream
T2259	8289-8291	IN	denotes	of
T2260	8292-8296	NN	denotes	cell
T2261	8297-8301	NN	denotes	fate
T2262	8302-8315	NN	denotes	specification
T2263	8315-8317	,	denotes	,
T2264	8317-8319	IN	denotes	at
T2265	8320-8321	DT	denotes	a
T2266	8322-8327	NN	denotes	stage
T2267	8328-8333	WRB	denotes	where
T2269	8334-8337	DT	denotes	the
T2270	8338-8341	NN	denotes	bud
T2268	8342-8348	VBZ	denotes	begins
T2271	8349-8351	TO	denotes	to
T2272	8352-8359	VB	denotes	exhibit
T2273	8360-8368	VBN	denotes	enhanced
T2274	8369-8382	NN	denotes	proliferation
T2275	8383-8386	CC	denotes	and
T2276	8387-8396	NN	denotes	migration
T2277	8396-8397	.	denotes	.
T2702	8408-8413	NN	denotes	Snail
T2703	8414-8418	NN	denotes	mRNA
T2705	8419-8422	CC	denotes	and
T2706	8423-8430	NN	denotes	Protein
T2707	8431-8434	VBP	denotes	Are
T2704	8435-8444	VBN	denotes	Expressed
T2708	8445-8456	RB	denotes	Transiently
T2709	8457-8459	IN	denotes	at
T2710	8460-8463	DT	denotes	the
T2712	8464-8468	NN	denotes	Hair
T2713	8469-8472	NN	denotes	Bud
T2711	8473-8478	NN	denotes	Stage
T2714	8479-8481	IN	denotes	of
T2715	8482-8490	NN	denotes	Follicle
T2716	8491-8504	NN	denotes	Morphogenesis
T2717	8504-8721	sentence	denotes	Although Snail family members are most frequently associated with EMTs, they also participate in many malignant processes involving a down-regulation but not a quantitative abrogation of intercellular junctions [18].
T2718	8505-8513	IN	denotes	Although
T2720	8514-8519	NN	denotes	Snail
T2722	8520-8526	NN	denotes	family
T2721	8527-8534	NNS	denotes	members
T2723	8535-8538	VBP	denotes	are
T2724	8539-8543	RBS	denotes	most
T2725	8544-8554	RB	denotes	frequently
T2719	8555-8565	VBN	denotes	associated
T2727	8566-8570	IN	denotes	with
T2728	8571-8575	NNS	denotes	EMTs
T2729	8575-8577	,	denotes	,
T2730	8577-8581	PRP	denotes	they
T2731	8582-8586	RB	denotes	also
T2726	8587-8598	VBP	denotes	participate
T2732	8599-8601	IN	denotes	in
T2733	8602-8606	JJ	denotes	many
T2735	8607-8616	JJ	denotes	malignant
T2734	8617-8626	NNS	denotes	processes
T2736	8627-8636	VBG	denotes	involving
T2737	8637-8638	DT	denotes	a
T2739	8639-8643	JJ	denotes	down
T2740	8643-8644	HYPH	denotes	-
T2738	8644-8654	NN	denotes	regulation
T2741	8655-8658	CC	denotes	but
T2742	8659-8662	RB	denotes	not
T2743	8663-8664	DT	denotes	a
T2745	8665-8677	JJ	denotes	quantitative
T2744	8678-8688	NN	denotes	abrogation
T2746	8689-8691	IN	denotes	of
T2747	8692-8705	JJ	denotes	intercellular
T2748	8706-8715	NNS	denotes	junctions
T2749	8716-8717	-LRB-	denotes	[
T2750	8717-8719	CD	denotes	18
T2751	8719-8720	-RRB-	denotes	]
T2752	8720-8721	.	denotes	.
T2753	8721-8906	sentence	denotes	The range of developmental processes in which Snail family members have been implicated thus includes the type of epithelial remodeling that is observed in hair follicle bud formation.
T2754	8722-8725	DT	denotes	The
T2755	8726-8731	NN	denotes	range
T2757	8732-8734	IN	denotes	of
T2758	8735-8748	JJ	denotes	developmental
T2759	8749-8758	NNS	denotes	processes
T2760	8759-8761	IN	denotes	in
T2762	8762-8767	WDT	denotes	which
T2763	8768-8773	NN	denotes	Snail
T2765	8774-8780	NN	denotes	family
T2764	8781-8788	NNS	denotes	members
T2766	8789-8793	VBP	denotes	have
T2767	8794-8798	VBN	denotes	been
T2761	8799-8809	VBN	denotes	implicated
T2768	8810-8814	RB	denotes	thus
T2756	8815-8823	VBZ	denotes	includes
T2769	8824-8827	DT	denotes	the
T2770	8828-8832	NN	denotes	type
T2771	8833-8835	IN	denotes	of
T2772	8836-8846	JJ	denotes	epithelial
T2773	8847-8857	NN	denotes	remodeling
T2774	8858-8862	WDT	denotes	that
T2776	8863-8865	VBZ	denotes	is
T2775	8866-8874	VBN	denotes	observed
T2777	8875-8877	IN	denotes	in
T2778	8878-8882	NN	denotes	hair
T2779	8883-8891	NN	denotes	follicle
T2780	8892-8895	NN	denotes	bud
T2781	8896-8905	NN	denotes	formation
T2782	8905-8906	.	denotes	.
T2783	8906-9260	sentence	denotes	Given our prior observation that exogenously added Snail can participate with LEF-1/β-catenin in down-regulating E-cadherin expression in keratinocytes [4], coupled with the established requirement for LEF-1/β-catenin in hair follicle morphogenesis [4,19], we turned to addressing whether Snail/Slug family members might also participate in the process.
T2784	8907-8912	VBN	denotes	Given
T2786	8913-8916	PRP$	denotes	our
T2788	8917-8922	JJ	denotes	prior
T2787	8923-8934	NN	denotes	observation
T2789	8935-8939	IN	denotes	that
T2791	8940-8951	RB	denotes	exogenously
T2792	8952-8957	VBN	denotes	added
T2793	8958-8963	NN	denotes	Snail
T2794	8964-8967	MD	denotes	can
T2790	8968-8979	VB	denotes	participate
T2795	8980-8984	IN	denotes	with
T2796	8985-8988	NN	denotes	LEF
T2797	8988-8989	HYPH	denotes	-
T2798	8989-8990	CD	denotes	1
T2799	8990-8991	HYPH	denotes	/
T2800	8991-8992	NN	denotes	β
T2802	8992-8993	HYPH	denotes	-
T2801	8993-9000	NN	denotes	catenin
T2803	9001-9003	IN	denotes	in
T2804	9004-9008	RB	denotes	down
T2806	9008-9009	HYPH	denotes	-
T2805	9009-9019	VBG	denotes	regulating
T2807	9020-9021	NN	denotes	E
T2809	9021-9022	HYPH	denotes	-
T2808	9022-9030	NN	denotes	cadherin
T2810	9031-9041	NN	denotes	expression
T2811	9042-9044	IN	denotes	in
T2812	9045-9058	NNS	denotes	keratinocytes
T2813	9059-9060	-LRB-	denotes	[
T2814	9060-9061	CD	denotes	4
T2815	9061-9062	-RRB-	denotes	]
T2816	9062-9064	,	denotes	,
T2817	9064-9071	VBN	denotes	coupled
T2818	9072-9076	IN	denotes	with
T2819	9077-9080	DT	denotes	the
T2821	9081-9092	JJ	denotes	established
T2820	9093-9104	NN	denotes	requirement
T2822	9105-9108	IN	denotes	for
T2823	9109-9112	NN	denotes	LEF
T2824	9112-9113	HYPH	denotes	-
T2825	9113-9114	CD	denotes	1
T2826	9114-9115	HYPH	denotes	/
T2827	9115-9116	NN	denotes	β
T2829	9116-9117	HYPH	denotes	-
T2828	9117-9124	NN	denotes	catenin
T2830	9125-9127	IN	denotes	in
T2831	9128-9132	NN	denotes	hair
T2832	9133-9141	NN	denotes	follicle
T2833	9142-9155	NN	denotes	morphogenesis
T2834	9156-9157	-LRB-	denotes	[
T2836	9157-9158	CD	denotes	4
T2837	9158-9159	,	denotes	,
T2835	9159-9161	CD	denotes	19
T2838	9161-9162	-RRB-	denotes	]
T2839	9162-9164	,	denotes	,
T2840	9164-9166	PRP	denotes	we
T2785	9167-9173	VBD	denotes	turned
T2841	9174-9176	IN	denotes	to
T2842	9177-9187	VBG	denotes	addressing
T2843	9188-9195	IN	denotes	whether
T2845	9196-9201	NN	denotes	Snail
T2847	9201-9202	HYPH	denotes	/
T2846	9202-9206	NN	denotes	Slug
T2849	9207-9213	NN	denotes	family
T2848	9214-9221	NNS	denotes	members
T2850	9222-9227	MD	denotes	might
T2851	9228-9232	RB	denotes	also
T2844	9233-9244	VB	denotes	participate
T2852	9245-9247	IN	denotes	in
T2853	9248-9251	DT	denotes	the
T2854	9252-9259	NN	denotes	process
T2855	9259-9260	.	denotes	.
T2856	9260-9415	sentence	denotes	PCR analyses identified transient Snail mRNA expression during a period of skin embryogenesis when waves of hair follicles are forming (unpublished data).
T2857	9261-9264	NN	denotes	PCR
T2858	9265-9273	NNS	denotes	analyses
T2859	9274-9284	VBD	denotes	identified
T2860	9285-9294	JJ	denotes	transient
T2862	9295-9300	NN	denotes	Snail
T2863	9301-9305	NN	denotes	mRNA
T2861	9306-9316	NN	denotes	expression
T2864	9317-9323	IN	denotes	during
T2865	9324-9325	DT	denotes	a
T2866	9326-9332	NN	denotes	period
T2867	9333-9335	IN	denotes	of
T2868	9336-9340	NN	denotes	skin
T2869	9341-9354	NN	denotes	embryogenesis
T2870	9355-9359	WRB	denotes	when
T2872	9360-9365	NNS	denotes	waves
T2873	9366-9368	IN	denotes	of
T2874	9369-9373	NN	denotes	hair
T2875	9374-9383	NNS	denotes	follicles
T2876	9384-9387	VBP	denotes	are
T2871	9388-9395	VBG	denotes	forming
T2877	9396-9397	-LRB-	denotes	(
T2879	9397-9408	JJ	denotes	unpublished
T2878	9409-9413	NNS	denotes	data
T2880	9413-9414	-RRB-	denotes	)
T2881	9414-9415	.	denotes	.
T2882	9415-9417	TO	denotes	To
T2884	9415-9597	sentence	denotes	To pinpoint specifically where Snail mRNA is expressed in the developing skin, we conducted in situ hybridization using a cRNA probe unique to the Snail 3′ untranslated region (UTR).
T2883	9418-9426	VB	denotes	pinpoint
T2886	9427-9439	RB	denotes	specifically
T2887	9440-9445	WRB	denotes	where
T2889	9446-9451	NN	denotes	Snail
T2890	9452-9456	NN	denotes	mRNA
T2891	9457-9459	VBZ	denotes	is
T2888	9460-9469	VBN	denotes	expressed
T2892	9470-9472	IN	denotes	in
T2893	9473-9476	DT	denotes	the
T2895	9477-9487	VBG	denotes	developing
T2894	9488-9492	NN	denotes	skin
T2896	9492-9494	,	denotes	,
T2897	9494-9496	PRP	denotes	we
T2885	9497-9506	VBD	denotes	conducted
T2898	9507-9509	FW	denotes	in
T2899	9510-9514	FW	denotes	situ
T2900	9515-9528	NN	denotes	hybridization
T2901	9529-9534	VBG	denotes	using
T2902	9535-9536	DT	denotes	a
T2904	9537-9541	NN	denotes	cRNA
T2903	9542-9547	NN	denotes	probe
T2905	9548-9554	JJ	denotes	unique
T2906	9555-9557	IN	denotes	to
T2907	9558-9561	DT	denotes	the
T2909	9562-9567	NNP	denotes	Snail
T2910	9568-9569	CD	denotes	3
T2911	9569-9570	SYM	denotes	′
T2912	9571-9583	JJ	denotes	untranslated
T2908	9584-9590	NN	denotes	region
T2913	9591-9592	-LRB-	denotes	(
T2914	9592-9595	NN	denotes	UTR
T2915	9595-9596	-RRB-	denotes	)
T2916	9596-9597	.	denotes	.
T2917	9597-9786	sentence	denotes	Embryonic day 17.5 (E17.5) was chosen, since the multiple waves of follicle morphogenesis occurring at this time enabled us to evaluate Snail expression at different stages of the process.
T2918	9598-9607	JJ	denotes	Embryonic
T2919	9608-9611	NN	denotes	day
T2921	9612-9616	CD	denotes	17.5
T2922	9617-9618	-LRB-	denotes	(
T2923	9618-9623	NN	denotes	E17.5
T2924	9623-9624	-RRB-	denotes	)
T2925	9625-9628	VBD	denotes	was
T2920	9629-9635	VBN	denotes	chosen
T2926	9635-9637	,	denotes	,
T2927	9637-9642	IN	denotes	since
T2929	9643-9646	DT	denotes	the
T2931	9647-9655	JJ	denotes	multiple
T2930	9656-9661	NNS	denotes	waves
T2932	9662-9664	IN	denotes	of
T2933	9665-9673	NN	denotes	follicle
T2934	9674-9687	NN	denotes	morphogenesis
T2935	9688-9697	VBG	denotes	occurring
T2936	9698-9700	IN	denotes	at
T2937	9701-9705	DT	denotes	this
T2938	9706-9710	NN	denotes	time
T2928	9711-9718	VBD	denotes	enabled
T2939	9719-9721	PRP	denotes	us
T2940	9722-9724	TO	denotes	to
T2941	9725-9733	VB	denotes	evaluate
T2942	9734-9739	NN	denotes	Snail
T2943	9740-9750	NN	denotes	expression
T2944	9751-9753	IN	denotes	at
T2945	9754-9763	JJ	denotes	different
T2946	9764-9770	NNS	denotes	stages
T2947	9771-9773	IN	denotes	of
T2948	9774-9777	DT	denotes	the
T2949	9778-9785	NN	denotes	process
T2950	9785-9786	.	denotes	.
T2951	9786-9889	sentence	denotes	As shown in Figure 1A, specific hybridization was detected within the epithelium of nascent hair buds.
T2952	9787-9789	IN	denotes	As
T2953	9790-9795	VBN	denotes	shown
T2955	9796-9798	IN	denotes	in
T2956	9799-9805	NN	denotes	Figure
T2957	9806-9808	NN	denotes	1A
T2958	9808-9810	,	denotes	,
T2959	9810-9818	JJ	denotes	specific
T2960	9819-9832	NN	denotes	hybridization
T2961	9833-9836	VBD	denotes	was
T2954	9837-9845	VBN	denotes	detected
T2962	9846-9852	IN	denotes	within
T2963	9853-9856	DT	denotes	the
T2964	9857-9867	NN	denotes	epithelium
T2965	9868-9870	IN	denotes	of
T2966	9871-9878	JJ	denotes	nascent
T2968	9879-9883	NN	denotes	hair
T2967	9884-9888	NNS	denotes	buds
T2969	9888-9889	.	denotes	.
T2970	9889-10043	sentence	denotes	By contrast, as follicles progressed further through their development (e.g., germ and peg stages), they exhibited no signs of hybridization (Figure 1A).
T2971	9890-9892	IN	denotes	By
T2973	9893-9901	NN	denotes	contrast
T2974	9901-9903	,	denotes	,
T2975	9903-9905	IN	denotes	as
T2977	9906-9915	NNS	denotes	follicles
T2976	9916-9926	VBD	denotes	progressed
T2978	9927-9934	RBR	denotes	further
T2979	9935-9942	IN	denotes	through
T2980	9943-9948	PRP$	denotes	their
T2981	9949-9960	NN	denotes	development
T2982	9961-9962	-LRB-	denotes	(
T2984	9962-9966	FW	denotes	e.g.
T2985	9966-9968	,	denotes	,
T2986	9968-9972	NN	denotes	germ
T2987	9973-9976	CC	denotes	and
T2988	9977-9980	NN	denotes	peg
T2983	9981-9987	NNS	denotes	stages
T2989	9987-9988	-RRB-	denotes	)
T2990	9988-9990	,	denotes	,
T2991	9990-9994	PRP	denotes	they
T2972	9995-10004	VBD	denotes	exhibited
T2992	10005-10007	DT	denotes	no
T2993	10008-10013	NNS	denotes	signs
T2994	10014-10016	IN	denotes	of
T2995	10017-10030	NN	denotes	hybridization
T2996	10031-10032	-LRB-	denotes	(
T2998	10032-10038	NN	denotes	Figure
T2997	10039-10041	NN	denotes	1A
T2999	10041-10042	-RRB-	denotes	)
T3000	10042-10043	.	denotes	.
T3001	10043-10250	sentence	denotes	The transient nature of Snail mRNA expression during follicle development was most apparent in hybridized skin sections containing follicles from two different waves of morphogenesis (as shown in Figure 1).
T3002	10044-10047	DT	denotes	The
T3004	10048-10057	JJ	denotes	transient
T3003	10058-10064	NN	denotes	nature
T3006	10065-10067	IN	denotes	of
T3007	10068-10073	NN	denotes	Snail
T3009	10074-10078	NN	denotes	mRNA
T3008	10079-10089	NN	denotes	expression
T3010	10090-10096	IN	denotes	during
T3011	10097-10105	NN	denotes	follicle
T3012	10106-10117	NN	denotes	development
T3005	10118-10121	VBD	denotes	was
T3013	10122-10126	RBS	denotes	most
T3014	10127-10135	JJ	denotes	apparent
T3015	10136-10138	IN	denotes	in
T3016	10139-10149	VBN	denotes	hybridized
T3018	10150-10154	NN	denotes	skin
T3017	10155-10163	NNS	denotes	sections
T3019	10164-10174	VBG	denotes	containing
T3020	10175-10184	NNS	denotes	follicles
T3021	10185-10189	IN	denotes	from
T3022	10190-10193	CD	denotes	two
T3024	10194-10203	JJ	denotes	different
T3023	10204-10209	NNS	denotes	waves
T3025	10210-10212	IN	denotes	of
T3026	10213-10226	NN	denotes	morphogenesis
T3027	10227-10228	-LRB-	denotes	(
T3029	10228-10230	IN	denotes	as
T3028	10231-10236	VBN	denotes	shown
T3030	10237-10239	IN	denotes	in
T3031	10240-10246	NN	denotes	Figure
T3032	10247-10248	CD	denotes	1
T3033	10248-10249	-RRB-	denotes	)
T3034	10249-10250	.	denotes	.
T3035	10250-10362	sentence	denotes	Hybridizing hair buds from a later wave appeared juxtaposed with nonhybridizing follicles from an earlier wave.
T3036	10251-10262	VBG	denotes	Hybridizing
T3038	10263-10267	NN	denotes	hair
T3037	10268-10272	NNS	denotes	buds
T3040	10273-10277	IN	denotes	from
T3041	10278-10279	DT	denotes	a
T3043	10280-10285	JJ	denotes	later
T3042	10286-10290	NN	denotes	wave
T3039	10291-10299	VBD	denotes	appeared
T3044	10300-10310	JJ	denotes	juxtaposed
T3045	10311-10315	IN	denotes	with
T3046	10316-10330	JJ	denotes	nonhybridizing
T3047	10331-10340	NNS	denotes	follicles
T3048	10341-10345	IN	denotes	from
T3049	10346-10348	DT	denotes	an
T3051	10349-10356	JJR	denotes	earlier
T3050	10357-10361	NN	denotes	wave
T3052	10361-10362	.	denotes	.
T3053	10362-12983	sentence	denotes	Figure 1 Snail Is Expressed Exclusively in the Hair Bud during Morphogenesis Embryos were either frozen in OCT embedding compound (A, F, and H) or embedded in paraffin (C, D, E, and G), and then sectioned (8 μm). (A) In situ hybridizations with Snail sense or antisense cRNA probes. Black dotted lines demarcate the basement membrane that separates the epidermis (epi) from dermis (der). Arrows point to Snail RNA expression, restricted to the hair bud stage of follicle morphogenesis. It was not seen in later hair germ or peg stages. (B) Expression of Snail protein coincides with hair development. Protein extracts were prepared from keratinocytes transfected with empty expression vector (K14), containing the K14 promoter or with the vector driving HA-tagged Snail (K14-Snail); or from whole skin from E13.5 to P5 animals, including newborn (nb). Equal amounts of proteins were then resolved by SDS-PAGE through 12% gels and subjected to Western blotting using either an affinity-purified Snail polyclonal antiserum, which we generated, or anti-tubulin (loading control). (C–E) Immunohistochemistry shows expression of Snail protein in the nuclei of cells within the hair and skin. (C) E13.5 skin with a single layered epidermis (epi) shows no Snail expression. (D) The first morphological sign that cells have adopted a hair follicle fate is a cluster of cells called a placode in E16.5 skin. Snail is not expressed at this stage of development. (E) Snail is expressed in the hair bud of E17.5 skin but not in later stages of development such as the germ or peg. (F) Immunofluorescence with anti-Ki67 (green) identifies the proliferating cells of the skin, restricted to the basal layer of the epidermis and developing hair follicles. Anti-β4 int labeling reveals the presence of the hemidesmosomal integrin β4, restricted to the base of cells adhering to the underlying basement membrane. The white dotted line marks the outermost surface of the skin. (G) Immunohistochemistry with pMAPK marks a subset of proliferating cells within the epidermis and hair bud. Anti-pMAPK labeling was consistently robust within the hair bud. (H) Immunofluorescence with anti-laminin 5 (lam5), which demarcates the basement membrane, and anti-E-cadherin (E-cad), a component of AJs. At the leading edge of the growing bud, cell-cell borders show markedly diminished anti-E-cadherin labeling (arrowheads). To determine whether this transient nature of Snail mRNA expression is reflected at the protein level, we generated an antibody against the N-terminal sequence that resides upstream of the more conserved zinc finger domains.
T3054	12759-12761	TO	denotes	To
T3055	12762-12771	VB	denotes	determine
T3057	12772-12779	IN	denotes	whether
T3059	12780-12784	DT	denotes	this
T3061	12785-12794	JJ	denotes	transient
T3060	12795-12801	NN	denotes	nature
T3062	12802-12804	IN	denotes	of
T3063	12805-12810	NN	denotes	Snail
T3065	12811-12815	NN	denotes	mRNA
T3064	12816-12826	NN	denotes	expression
T3066	12827-12829	VBZ	denotes	is
T3058	12830-12839	VBN	denotes	reflected
T3067	12840-12842	IN	denotes	at
T3068	12843-12846	DT	denotes	the
T3070	12847-12854	NN	denotes	protein
T3069	12855-12860	NN	denotes	level
T3071	12860-12862	,	denotes	,
T3072	12862-12864	PRP	denotes	we
T3056	12865-12874	VBD	denotes	generated
T3073	12875-12877	DT	denotes	an
T3074	12878-12886	NN	denotes	antibody
T3075	12887-12894	IN	denotes	against
T3076	12895-12898	DT	denotes	the
T3078	12899-12900	NN	denotes	N
T3080	12900-12901	HYPH	denotes	-
T3079	12901-12909	JJ	denotes	terminal
T3077	12910-12918	NN	denotes	sequence
T3081	12919-12923	WDT	denotes	that
T3082	12924-12931	VBZ	denotes	resides
T3083	12932-12940	RB	denotes	upstream
T3084	12941-12943	IN	denotes	of
T3085	12944-12947	DT	denotes	the
T3087	12948-12952	RBR	denotes	more
T3088	12953-12962	VBN	denotes	conserved
T3089	12963-12967	NN	denotes	zinc
T3090	12968-12974	NN	denotes	finger
T3086	12975-12982	NNS	denotes	domains
T3091	12982-12983	.	denotes	.
T3092	12983-13238	sentence	denotes	As judged by Western blot analysis, the antibody did not detect endogenous proteins from cultured keratinocytes, but it did yield a band of the expected size from keratinocytes transiently expressing a hemagglutinin (HA)-tagged Snail protein (Figure 1B).
T3093	12984-12986	IN	denotes	As
T3094	12987-12993	VBN	denotes	judged
T3096	12994-12996	IN	denotes	by
T3097	12997-13004	NNP	denotes	Western
T3098	13005-13009	NN	denotes	blot
T3099	13010-13018	NN	denotes	analysis
T3100	13018-13020	,	denotes	,
T3101	13020-13023	DT	denotes	the
T3102	13024-13032	NN	denotes	antibody
T3103	13033-13036	VBD	denotes	did
T3104	13037-13040	RB	denotes	not
T3095	13041-13047	VB	denotes	detect
T3105	13048-13058	JJ	denotes	endogenous
T3106	13059-13067	NN	denotes	proteins
T3107	13068-13072	IN	denotes	from
T3108	13073-13081	VBN	denotes	cultured
T3109	13082-13095	NNS	denotes	keratinocytes
T3110	13095-13097	,	denotes	,
T3111	13097-13100	CC	denotes	but
T3112	13101-13103	PRP	denotes	it
T3114	13104-13107	VBD	denotes	did
T3113	13108-13113	VB	denotes	yield
T3115	13114-13115	DT	denotes	a
T3116	13116-13120	NN	denotes	band
T3117	13121-13123	IN	denotes	of
T3118	13124-13127	DT	denotes	the
T3120	13128-13136	VBN	denotes	expected
T3119	13137-13141	NN	denotes	size
T3121	13142-13146	IN	denotes	from
T3122	13147-13160	NNS	denotes	keratinocytes
T3123	13161-13172	RB	denotes	transiently
T3124	13173-13183	VBG	denotes	expressing
T3125	13184-13185	DT	denotes	a
T3127	13186-13199	NN	denotes	hemagglutinin
T3129	13200-13201	-LRB-	denotes	(
T3130	13201-13203	NN	denotes	HA
T3131	13203-13204	-RRB-	denotes	)
T3132	13204-13205	HYPH	denotes	-
T3128	13205-13211	VBN	denotes	tagged
T3133	13212-13217	NN	denotes	Snail
T3126	13218-13225	NN	denotes	protein
T3134	13226-13227	-LRB-	denotes	(
T3136	13227-13233	NN	denotes	Figure
T3135	13234-13236	NN	denotes	1B
T3137	13236-13237	-RRB-	denotes	)
T3138	13237-13238	.	denotes	.
T3139	13238-13555	sentence	denotes	The antibody also recognized a band corresponding to the size of endogenous Snail (approximately 28 kDa) in lysates from embryonic mouse skin, the temporal appearance of which corresponded to the waves of hair follicle morphogenesis from E15.5 to newborn when over 90% of the hair on the mouse is formed (Figure 1B).
T3140	13239-13242	DT	denotes	The
T3141	13243-13251	NN	denotes	antibody
T3143	13252-13256	RB	denotes	also
T3142	13257-13267	VBD	denotes	recognized
T3144	13268-13269	DT	denotes	a
T3145	13270-13274	NN	denotes	band
T3146	13275-13288	VBG	denotes	corresponding
T3147	13289-13291	IN	denotes	to
T3148	13292-13295	DT	denotes	the
T3149	13296-13300	NN	denotes	size
T3150	13301-13303	IN	denotes	of
T3151	13304-13314	JJ	denotes	endogenous
T3152	13315-13320	NN	denotes	Snail
T3153	13321-13322	-LRB-	denotes	(
T3154	13322-13335	RB	denotes	approximately
T3155	13336-13338	CD	denotes	28
T3156	13339-13342	NN	denotes	kDa
T3157	13342-13343	-RRB-	denotes	)
T3158	13344-13346	IN	denotes	in
T3159	13347-13354	NNS	denotes	lysates
T3160	13355-13359	IN	denotes	from
T3161	13360-13369	JJ	denotes	embryonic
T3163	13370-13375	NN	denotes	mouse
T3162	13376-13380	NN	denotes	skin
T3164	13380-13382	,	denotes	,
T3165	13382-13385	DT	denotes	the
T3167	13386-13394	JJ	denotes	temporal
T3166	13395-13405	NN	denotes	appearance
T3169	13406-13408	IN	denotes	of
T3170	13409-13414	WDT	denotes	which
T3168	13415-13427	VBD	denotes	corresponded
T3171	13428-13430	IN	denotes	to
T3172	13431-13434	DT	denotes	the
T3173	13435-13440	NNS	denotes	waves
T3174	13441-13443	IN	denotes	of
T3175	13444-13448	NN	denotes	hair
T3176	13449-13457	NN	denotes	follicle
T3177	13458-13471	NN	denotes	morphogenesis
T3178	13472-13476	IN	denotes	from
T3179	13477-13482	NN	denotes	E15.5
T3180	13483-13485	IN	denotes	to
T3181	13486-13493	JJ	denotes	newborn
T3182	13494-13498	WRB	denotes	when
T3184	13499-13503	IN	denotes	over
T3185	13504-13506	CD	denotes	90
T3186	13506-13507	NN	denotes	%
T3187	13508-13510	IN	denotes	of
T3188	13511-13514	DT	denotes	the
T3189	13515-13519	NN	denotes	hair
T3190	13520-13522	IN	denotes	on
T3191	13523-13526	DT	denotes	the
T3192	13527-13532	NN	denotes	mouse
T3193	13533-13535	VBZ	denotes	is
T3183	13536-13542	VBN	denotes	formed
T3194	13543-13544	-LRB-	denotes	(
T3196	13544-13550	NN	denotes	Figure
T3195	13551-13553	NN	denotes	1B
T3197	13553-13554	-RRB-	denotes	)
T3198	13554-13555	.	denotes	.
T3199	13555-13849	sentence	denotes	Consistent with the Western blot data, immunohistochemical analysis did not detect Snail in single-layered E13.5 epidermis (Figure 1C) nor in the placode, which is the earliest morphological sign of the commitment of multipotent cells of the embryonic ectoderm to a hair cell fate (Figure 1D).
T3200	13556-13566	JJ	denotes	Consistent
T3202	13567-13571	IN	denotes	with
T3203	13572-13575	DT	denotes	the
T3205	13576-13583	NNP	denotes	Western
T3206	13584-13588	NN	denotes	blot
T3204	13589-13593	NNS	denotes	data
T3207	13593-13595	,	denotes	,
T3208	13595-13614	JJ	denotes	immunohistochemical
T3209	13615-13623	NN	denotes	analysis
T3210	13624-13627	VBD	denotes	did
T3211	13628-13631	RB	denotes	not
T3201	13632-13638	VB	denotes	detect
T3212	13639-13644	NN	denotes	Snail
T3213	13645-13647	IN	denotes	in
T3214	13648-13654	JJ	denotes	single
T3216	13654-13655	HYPH	denotes	-
T3215	13655-13662	JJ	denotes	layered
T3218	13663-13668	NN	denotes	E13.5
T3217	13669-13678	NN	denotes	epidermis
T3219	13679-13680	-LRB-	denotes	(
T3221	13680-13686	NN	denotes	Figure
T3220	13687-13689	NN	denotes	1C
T3222	13689-13690	-RRB-	denotes	)
T3223	13691-13694	CC	denotes	nor
T3224	13695-13697	IN	denotes	in
T3225	13698-13701	DT	denotes	the
T3226	13702-13709	NN	denotes	placode
T3227	13709-13711	,	denotes	,
T3228	13711-13716	WDT	denotes	which
T3229	13717-13719	VBZ	denotes	is
T3230	13720-13723	DT	denotes	the
T3232	13724-13732	JJS	denotes	earliest
T3233	13733-13746	JJ	denotes	morphological
T3231	13747-13751	NN	denotes	sign
T3234	13752-13754	IN	denotes	of
T3235	13755-13758	DT	denotes	the
T3236	13759-13769	NN	denotes	commitment
T3237	13770-13772	IN	denotes	of
T3238	13773-13784	JJ	denotes	multipotent
T3239	13785-13790	NNS	denotes	cells
T3240	13791-13793	IN	denotes	of
T3241	13794-13797	DT	denotes	the
T3243	13798-13807	JJ	denotes	embryonic
T3242	13808-13816	NN	denotes	ectoderm
T3244	13817-13819	IN	denotes	to
T3245	13820-13821	DT	denotes	a
T3247	13822-13826	NN	denotes	hair
T3248	13827-13831	NN	denotes	cell
T3246	13832-13836	NN	denotes	fate
T3249	13837-13838	-LRB-	denotes	(
T3251	13838-13844	NN	denotes	Figure
T3250	13845-13847	NN	denotes	1D
T3252	13847-13848	-RRB-	denotes	)
T3253	13848-13849	.	denotes	.
T3254	13849-14011	sentence	denotes	Consistent with the in situ hybridization results, anti-Snail antibody labeled only hair buds and not follicles at more mature stages of development (Figure 1E).
T3255	13850-13860	JJ	denotes	Consistent
T3257	13861-13865	IN	denotes	with
T3258	13866-13869	DT	denotes	the
T3260	13870-13872	FW	denotes	in
T3261	13873-13877	FW	denotes	situ
T3262	13878-13891	NN	denotes	hybridization
T3259	13892-13899	NNS	denotes	results
T3263	13899-13901	,	denotes	,
T3264	13901-13911	JJ	denotes	anti-Snail
T3265	13912-13920	NN	denotes	antibody
T3256	13921-13928	VBD	denotes	labeled
T3266	13929-13933	RB	denotes	only
T3268	13934-13938	NN	denotes	hair
T3267	13939-13943	NNS	denotes	buds
T3269	13944-13947	CC	denotes	and
T3270	13948-13951	RB	denotes	not
T3271	13952-13961	NNS	denotes	follicles
T3272	13962-13964	IN	denotes	at
T3273	13965-13969	RBR	denotes	more
T3275	13970-13976	JJ	denotes	mature
T3274	13977-13983	NNS	denotes	stages
T3276	13984-13986	IN	denotes	of
T3277	13987-13998	NN	denotes	development
T3278	13999-14000	-LRB-	denotes	(
T3280	14000-14006	NN	denotes	Figure
T3279	14007-14009	NN	denotes	1E
T3281	14009-14010	-RRB-	denotes	)
T3282	14010-14011	.	denotes	.
T3283	14011-14099	sentence	denotes	Taken together, the anti-Snail antibody appeared to be specific for its target protein.
T3284	14012-14017	VBN	denotes	Taken
T3286	14018-14026	RB	denotes	together
T3287	14026-14028	,	denotes	,
T3288	14028-14031	DT	denotes	the
T3290	14032-14042	JJ	denotes	anti-Snail
T3289	14043-14051	NN	denotes	antibody
T3285	14052-14060	VBD	denotes	appeared
T3291	14061-14063	TO	denotes	to
T3292	14064-14066	VB	denotes	be
T3293	14067-14075	JJ	denotes	specific
T3294	14076-14079	IN	denotes	for
T3295	14080-14083	PRP$	denotes	its
T3297	14084-14090	NN	denotes	target
T3296	14091-14098	NN	denotes	protein
T3298	14098-14099	.	denotes	.
T3299	14099-14215	sentence	denotes	It did not detect other Snail family members known to be expressed in keratinocytes and/or skin (unpublished data).
T3300	14100-14102	PRP	denotes	It
T3302	14103-14106	VBD	denotes	did
T3303	14107-14110	RB	denotes	not
T3301	14111-14117	VB	denotes	detect
T3304	14118-14123	JJ	denotes	other
T3306	14124-14129	NN	denotes	Snail
T3307	14130-14136	NN	denotes	family
T3305	14137-14144	NNS	denotes	members
T3308	14145-14150	VBN	denotes	known
T3309	14151-14153	TO	denotes	to
T3311	14154-14156	VB	denotes	be
T3310	14157-14166	VBN	denotes	expressed
T3312	14167-14169	IN	denotes	in
T3313	14170-14183	NNS	denotes	keratinocytes
T3314	14184-14187	CC	denotes	and
T3315	14187-14188	HYPH	denotes	/
T3316	14188-14190	CC	denotes	or
T3317	14191-14195	NN	denotes	skin
T3318	14196-14197	-LRB-	denotes	(
T3320	14197-14208	JJ	denotes	unpublished
T3319	14209-14213	NNS	denotes	data
T3321	14213-14214	-RRB-	denotes	)
T3322	14214-14215	.	denotes	.
T3323	14215-14377	sentence	denotes	Furthermore, the immunohistochemical data paralleled our Snail in situ hybridization data revealing transient Snail expression at the hair bud stage (Figure 1A).
T3324	14216-14227	RB	denotes	Furthermore
T3326	14227-14229	,	denotes	,
T3327	14229-14232	DT	denotes	the
T3329	14233-14252	JJ	denotes	immunohistochemical
T3328	14253-14257	NNS	denotes	data
T3325	14258-14268	VBD	denotes	paralleled
T3330	14269-14272	PRP$	denotes	our
T3332	14273-14278	NN	denotes	Snail
T3334	14279-14281	FW	denotes	in
T3335	14282-14286	FW	denotes	situ
T3333	14287-14300	NN	denotes	hybridization
T3331	14301-14305	NNS	denotes	data
T3336	14306-14315	VBG	denotes	revealing
T3337	14316-14325	JJ	denotes	transient
T3339	14326-14331	NN	denotes	Snail
T3338	14332-14342	NN	denotes	expression
T3340	14343-14345	IN	denotes	at
T3341	14346-14349	DT	denotes	the
T3343	14350-14354	NN	denotes	hair
T3344	14355-14358	NN	denotes	bud
T3342	14359-14364	NN	denotes	stage
T3345	14365-14366	-LRB-	denotes	(
T3347	14366-14372	NN	denotes	Figure
T3346	14373-14375	NN	denotes	1A
T3348	14375-14376	-RRB-	denotes	)
T3349	14376-14377	.	denotes	.
T3350	14377-14489	sentence	denotes	As judged by immunohistochemistry, Snail protein was localized to the nuclei of the hair bud cells (Figure 1E).
T3351	14378-14380	IN	denotes	As
T3352	14381-14387	VBN	denotes	judged
T3354	14388-14390	IN	denotes	by
T3355	14391-14411	NN	denotes	immunohistochemistry
T3356	14411-14413	,	denotes	,
T3357	14413-14418	NN	denotes	Snail
T3358	14419-14426	NN	denotes	protein
T3359	14427-14430	VBD	denotes	was
T3353	14431-14440	VBN	denotes	localized
T3360	14441-14443	IN	denotes	to
T3361	14444-14447	DT	denotes	the
T3362	14448-14454	NNS	denotes	nuclei
T3363	14455-14457	IN	denotes	of
T3364	14458-14461	DT	denotes	the
T3366	14462-14466	NN	denotes	hair
T3367	14467-14470	NN	denotes	bud
T3365	14471-14476	NNS	denotes	cells
T3368	14477-14478	-LRB-	denotes	(
T3370	14478-14484	NN	denotes	Figure
T3369	14485-14487	NN	denotes	1E
T3371	14487-14488	-RRB-	denotes	)
T3372	14488-14489	.	denotes	.
T3373	14489-14585	sentence	denotes	This feature was consistent with Snail's known function as a transcriptional repressor [12,13].
T3374	14490-14494	DT	denotes	This
T3375	14495-14502	NN	denotes	feature
T3376	14503-14506	VBD	denotes	was
T3377	14507-14517	JJ	denotes	consistent
T3378	14518-14522	IN	denotes	with
T3379	14523-14528	NN	denotes	Snail
T3381	14528-14530	POS	denotes	's
T3382	14531-14536	VBN	denotes	known
T3380	14537-14545	NN	denotes	function
T3383	14546-14548	IN	denotes	as
T3384	14549-14550	DT	denotes	a
T3386	14551-14566	JJ	denotes	transcriptional
T3385	14567-14576	NN	denotes	repressor
T3387	14577-14578	-LRB-	denotes	[
T3389	14578-14580	CD	denotes	12
T3390	14580-14581	,	denotes	,
T3388	14581-14583	CD	denotes	13
T3391	14583-14584	-RRB-	denotes	]
T3392	14584-14585	.	denotes	.
T3393	14585-14697	sentence	denotes	Additionally, anti-Snail labeling was detected in only three of the four major waves of follicle morphogenesis.
T3394	14586-14598	RB	denotes	Additionally
T3396	14598-14600	,	denotes	,
T3397	14600-14610	JJ	denotes	anti-Snail
T3398	14611-14619	NN	denotes	labeling
T3399	14620-14623	VBD	denotes	was
T3395	14624-14632	VBN	denotes	detected
T3400	14633-14635	IN	denotes	in
T3401	14636-14640	RB	denotes	only
T3403	14641-14646	CD	denotes	three
T3404	14647-14649	IN	denotes	of
T3405	14650-14653	DT	denotes	the
T3402	14654-14658	CD	denotes	four
T3407	14659-14664	JJ	denotes	major
T3406	14665-14670	NNS	denotes	waves
T3408	14671-14673	IN	denotes	of
T3409	14674-14682	NN	denotes	follicle
T3410	14683-14696	NN	denotes	morphogenesis
T3411	14696-14697	.	denotes	.
T3412	14697-14871	sentence	denotes	Snail was not found in the buds of guard hairs that are the earliest of all hairs to form (at E13.5), and which constitute less than 5% of the mouse coat (unpublished data).
T3413	14698-14703	NN	denotes	Snail
T3415	14704-14707	VBD	denotes	was
T3416	14708-14711	RB	denotes	not
T3414	14712-14717	VBN	denotes	found
T3417	14718-14720	IN	denotes	in
T3418	14721-14724	DT	denotes	the
T3419	14725-14729	NNS	denotes	buds
T3420	14730-14732	IN	denotes	of
T3421	14733-14738	NN	denotes	guard
T3422	14739-14744	NNS	denotes	hairs
T3423	14745-14749	WDT	denotes	that
T3424	14750-14753	VBP	denotes	are
T3425	14754-14757	DT	denotes	the
T3426	14758-14766	JJS	denotes	earliest
T3427	14767-14769	IN	denotes	of
T3428	14770-14773	DT	denotes	all
T3429	14774-14779	NNS	denotes	hairs
T3430	14780-14782	TO	denotes	to
T3431	14783-14787	VB	denotes	form
T3432	14788-14789	-LRB-	denotes	(
T3433	14789-14791	IN	denotes	at
T3434	14792-14797	NN	denotes	E13.5
T3435	14797-14798	-RRB-	denotes	)
T3436	14798-14800	,	denotes	,
T3437	14800-14803	CC	denotes	and
T3438	14804-14809	WDT	denotes	which
T3439	14810-14820	VBP	denotes	constitute
T3440	14821-14825	JJR	denotes	less
T3442	14826-14830	IN	denotes	than
T3441	14831-14832	CD	denotes	5
T3443	14832-14833	NN	denotes	%
T3444	14834-14836	IN	denotes	of
T3445	14837-14840	DT	denotes	the
T3447	14841-14846	NN	denotes	mouse
T3446	14847-14851	NN	denotes	coat
T3448	14852-14853	-LRB-	denotes	(
T3450	14853-14864	JJ	denotes	unpublished
T3449	14865-14869	NNS	denotes	data
T3451	14869-14870	-RRB-	denotes	)
T3452	14870-14871	.	denotes	.
T3453	14871-15112	sentence	denotes	As judged by immunofluorescence with antibodies against the proliferating nuclear antigen Ki67, the timing of Snail expression coincided with the stage at which the developing follicle enhanced its proliferation and down-growth (Figure 1F).
T3454	14872-14874	IN	denotes	As
T3455	14875-14881	VBN	denotes	judged
T3457	14882-14884	IN	denotes	by
T3458	14885-14903	NN	denotes	immunofluorescence
T3459	14904-14908	IN	denotes	with
T3460	14909-14919	NNS	denotes	antibodies
T3461	14920-14927	IN	denotes	against
T3462	14928-14931	DT	denotes	the
T3464	14932-14945	VBG	denotes	proliferating
T3465	14946-14953	JJ	denotes	nuclear
T3463	14954-14961	NN	denotes	antigen
T3466	14962-14966	NN	denotes	Ki67
T3467	14966-14968	,	denotes	,
T3468	14968-14971	DT	denotes	the
T3469	14972-14978	NN	denotes	timing
T3470	14979-14981	IN	denotes	of
T3471	14982-14987	NN	denotes	Snail
T3472	14988-14998	NN	denotes	expression
T3456	14999-15008	VBD	denotes	coincided
T3473	15009-15013	IN	denotes	with
T3474	15014-15017	DT	denotes	the
T3475	15018-15023	NN	denotes	stage
T3476	15024-15026	IN	denotes	at
T3478	15027-15032	WDT	denotes	which
T3479	15033-15036	DT	denotes	the
T3481	15037-15047	VBG	denotes	developing
T3480	15048-15056	NN	denotes	follicle
T3477	15057-15065	VBD	denotes	enhanced
T3482	15066-15069	PRP$	denotes	its
T3483	15070-15083	NN	denotes	proliferation
T3484	15084-15087	CC	denotes	and
T3485	15088-15092	JJ	denotes	down
T3487	15092-15093	HYPH	denotes	-
T3486	15093-15099	NN	denotes	growth
T3488	15100-15101	-LRB-	denotes	(
T3490	15101-15107	NN	denotes	Figure
T3489	15108-15110	NN	denotes	1F
T3491	15110-15111	-RRB-	denotes	)
T3492	15111-15112	.	denotes	.
T3493	15112-15334	sentence	denotes	Immunohistochemistry with antibodies against the active (phosphorylated) form of MAPK (pMAPK) marked a subset of the proliferating (Ki67-positive) cells, and pMAPK-positive cells were enriched in the hair bud (Figure 1G).
T3494	15113-15133	NN	denotes	Immunohistochemistry
T3496	15134-15138	IN	denotes	with
T3497	15139-15149	NNS	denotes	antibodies
T3498	15150-15157	IN	denotes	against
T3499	15158-15161	DT	denotes	the
T3501	15162-15168	JJ	denotes	active
T3502	15169-15170	-LRB-	denotes	(
T3503	15170-15184	VBN	denotes	phosphorylated
T3504	15184-15185	-RRB-	denotes	)
T3500	15186-15190	NN	denotes	form
T3505	15191-15193	IN	denotes	of
T3506	15194-15198	NN	denotes	MAPK
T3507	15199-15200	-LRB-	denotes	(
T3508	15200-15205	NN	denotes	pMAPK
T3509	15205-15206	-RRB-	denotes	)
T3495	15207-15213	VBD	denotes	marked
T3510	15214-15215	DT	denotes	a
T3511	15216-15222	NN	denotes	subset
T3512	15223-15225	IN	denotes	of
T3513	15226-15229	DT	denotes	the
T3515	15230-15243	VBG	denotes	proliferating
T3516	15244-15245	-LRB-	denotes	(
T3517	15245-15249	NN	denotes	Ki67
T3519	15249-15250	HYPH	denotes	-
T3518	15250-15258	JJ	denotes	positive
T3520	15258-15259	-RRB-	denotes	)
T3514	15260-15265	NNS	denotes	cells
T3521	15265-15267	,	denotes	,
T3522	15267-15270	CC	denotes	and
T3523	15271-15276	NN	denotes	pMAPK
T3525	15276-15277	HYPH	denotes	-
T3524	15277-15285	JJ	denotes	positive
T3526	15286-15291	NNS	denotes	cells
T3528	15292-15296	VBD	denotes	were
T3527	15297-15305	VBN	denotes	enriched
T3529	15306-15308	IN	denotes	in
T3530	15309-15312	DT	denotes	the
T3532	15313-15317	NN	denotes	hair
T3531	15318-15321	NN	denotes	bud
T3533	15322-15323	-LRB-	denotes	(
T3535	15323-15329	NN	denotes	Figure
T3534	15330-15332	NN	denotes	1G
T3536	15332-15333	-RRB-	denotes	)
T3537	15333-15334	.	denotes	.
T3538	15334-15530	sentence	denotes	The timing of Snail induction and Ki67 and pMAPK enrichment in the hair bud appeared to follow closely the induction of LEF-1/β-catenin activity, known to initiate in the hair placode stage [20].
T3539	15335-15338	DT	denotes	The
T3540	15339-15345	NN	denotes	timing
T3542	15346-15348	IN	denotes	of
T3543	15349-15354	NN	denotes	Snail
T3544	15355-15364	NN	denotes	induction
T3545	15365-15368	CC	denotes	and
T3546	15369-15373	NN	denotes	Ki67
T3548	15374-15377	CC	denotes	and
T3549	15378-15383	NN	denotes	pMAPK
T3547	15384-15394	NN	denotes	enrichment
T3550	15395-15397	IN	denotes	in
T3551	15398-15401	DT	denotes	the
T3553	15402-15406	NN	denotes	hair
T3552	15407-15410	NN	denotes	bud
T3541	15411-15419	VBD	denotes	appeared
T3554	15420-15422	TO	denotes	to
T3555	15423-15429	VB	denotes	follow
T3556	15430-15437	RB	denotes	closely
T3557	15438-15441	DT	denotes	the
T3558	15442-15451	NN	denotes	induction
T3559	15452-15454	IN	denotes	of
T3560	15455-15458	NN	denotes	LEF
T3562	15458-15459	HYPH	denotes	-
T3563	15459-15460	CD	denotes	1
T3564	15460-15461	HYPH	denotes	/
T3565	15461-15462	NN	denotes	β
T3567	15462-15463	HYPH	denotes	-
T3566	15463-15470	NN	denotes	catenin
T3561	15471-15479	NN	denotes	activity
T3568	15479-15481	,	denotes	,
T3569	15481-15486	VBN	denotes	known
T3570	15487-15489	TO	denotes	to
T3571	15490-15498	VB	denotes	initiate
T3572	15499-15501	IN	denotes	in
T3573	15502-15505	DT	denotes	the
T3575	15506-15510	NN	denotes	hair
T3576	15511-15518	NN	denotes	placode
T3574	15519-15524	NN	denotes	stage
T3577	15525-15526	-LRB-	denotes	[
T3578	15526-15528	CD	denotes	20
T3579	15528-15529	-RRB-	denotes	]
T3580	15529-15530	.	denotes	.
T3581	15530-15642	sentence	denotes	However, like placodes, hair buds exhibited down-regulation in E-cadherin expression (Figure 1H; see also [4]).
T3582	15531-15538	RB	denotes	However
T3584	15538-15540	,	denotes	,
T3585	15540-15544	IN	denotes	like
T3586	15545-15553	NNS	denotes	placodes
T3587	15553-15555	,	denotes	,
T3588	15555-15559	NN	denotes	hair
T3589	15560-15564	NNS	denotes	buds
T3583	15565-15574	VBD	denotes	exhibited
T3590	15575-15579	JJ	denotes	down
T3592	15579-15580	HYPH	denotes	-
T3591	15580-15590	NN	denotes	regulation
T3593	15591-15593	IN	denotes	in
T3594	15594-15595	NN	denotes	E
T3596	15595-15596	HYPH	denotes	-
T3595	15596-15604	NN	denotes	cadherin
T3597	15605-15615	NN	denotes	expression
T3598	15616-15617	-LRB-	denotes	(
T3600	15617-15623	NN	denotes	Figure
T3599	15624-15626	NN	denotes	1H
T3601	15626-15627	:	denotes	;
T3602	15628-15631	VB	denotes	see
T3603	15632-15636	RB	denotes	also
T3604	15637-15638	-LRB-	denotes	[
T3605	15638-15639	CD	denotes	4
T3606	15639-15640	-RRB-	denotes	]
T3607	15640-15641	-RRB-	denotes	)
T3608	15641-15642	.	denotes	.
T3910	15644-15653	JJ	denotes	Sustained
T3911	15654-15664	NN	denotes	Expression
T3913	15665-15667	IN	denotes	of
T3914	15668-15673	NN	denotes	Snail
T3912	15674-15681	VBZ	denotes	Results
T3915	15682-15684	IN	denotes	in
T3916	15685-15694	JJ	denotes	Epidermal
T3917	15695-15713	NN	denotes	Hyperproliferation
T3918	15714-15717	CC	denotes	and
T3919	15718-15733	NN	denotes	Differentiation
T3920	15734-15741	NNS	denotes	Defects
T3921	15742-15744	IN	denotes	in
T3922	15745-15747	NN	denotes	Tg
T3924	15748-15753	NN	denotes	Mouse
T3923	15754-15758	NN	denotes	Skin
T3925	15758-15966	sentence	denotes	The striking spike of Snail expression coincident with hair bud formation and enhanced proliferation prompted us to examine the consequences of ectopically expressing Snail elsewhere in mouse skin epidermis.
T3926	15759-15762	DT	denotes	The
T3928	15763-15771	JJ	denotes	striking
T3927	15772-15777	NN	denotes	spike
T3930	15778-15780	IN	denotes	of
T3931	15781-15786	NN	denotes	Snail
T3932	15787-15797	NN	denotes	expression
T3933	15798-15808	JJ	denotes	coincident
T3934	15809-15813	IN	denotes	with
T3935	15814-15818	NN	denotes	hair
T3936	15819-15822	NN	denotes	bud
T3937	15823-15832	NN	denotes	formation
T3938	15833-15836	CC	denotes	and
T3939	15837-15845	VBN	denotes	enhanced
T3940	15846-15859	NN	denotes	proliferation
T3929	15860-15868	VBD	denotes	prompted
T3941	15869-15871	PRP	denotes	us
T3942	15872-15874	TO	denotes	to
T3943	15875-15882	VB	denotes	examine
T3944	15883-15886	DT	denotes	the
T3945	15887-15899	NNS	denotes	consequences
T3946	15900-15902	IN	denotes	of
T3947	15903-15914	RB	denotes	ectopically
T3948	15915-15925	VBG	denotes	expressing
T3949	15926-15931	NN	denotes	Snail
T3950	15932-15941	RB	denotes	elsewhere
T3951	15942-15944	IN	denotes	in
T3952	15945-15950	NN	denotes	mouse
T3954	15951-15955	NN	denotes	skin
T3953	15956-15965	NN	denotes	epidermis
T3955	15965-15966	.	denotes	.
T3956	15966-16100	sentence	denotes	To distinguish Tg from endogenous Snail, we used the HA-epitope, shown previously not to alter Snail's transcriptional activity [12].
T3957	15967-15969	TO	denotes	To
T3958	15970-15981	VB	denotes	distinguish
T3960	15982-15984	NN	denotes	Tg
T3961	15985-15989	IN	denotes	from
T3962	15990-16000	JJ	denotes	endogenous
T3963	16001-16006	NN	denotes	Snail
T3964	16006-16008	,	denotes	,
T3965	16008-16010	PRP	denotes	we
T3959	16011-16015	VBD	denotes	used
T3966	16016-16019	DT	denotes	the
T3968	16020-16022	NN	denotes	HA
T3969	16022-16023	HYPH	denotes	-
T3967	16023-16030	NN	denotes	epitope
T3970	16030-16032	,	denotes	,
T3971	16032-16037	VBN	denotes	shown
T3972	16038-16048	RB	denotes	previously
T3973	16049-16052	RB	denotes	not
T3975	16053-16055	TO	denotes	to
T3974	16056-16061	VB	denotes	alter
T3976	16062-16067	NNP	denotes	Snail
T3978	16067-16069	POS	denotes	's
T3979	16070-16085	JJ	denotes	transcriptional
T3977	16086-16094	NN	denotes	activity
T3980	16095-16096	-LRB-	denotes	[
T3981	16096-16098	CD	denotes	12
T3982	16098-16099	-RRB-	denotes	]
T3983	16099-16100	.	denotes	.
T3984	16100-16212	sentence	denotes	Of 20 K14-Snail[HA] Tg animals generated, three expressed the transgene and all exhibited analogous phenotypes.
T3985	16101-16103	IN	denotes	Of
T3987	16104-16106	CD	denotes	20
T3989	16107-16110	NN	denotes	K14
T3991	16110-16111	HYPH	denotes	-
T3990	16111-16116	NN	denotes	Snail
T3992	16116-16117	-LRB-	denotes	[
T3993	16117-16119	NN	denotes	HA
T3994	16119-16120	-RRB-	denotes	]
T3995	16121-16123	NN	denotes	Tg
T3988	16124-16131	NNS	denotes	animals
T3996	16132-16141	VBN	denotes	generated
T3997	16141-16143	,	denotes	,
T3998	16143-16148	CD	denotes	three
T3986	16149-16158	VBD	denotes	expressed
T3999	16159-16162	DT	denotes	the
T4000	16163-16172	NN	denotes	transgene
T4001	16173-16176	CC	denotes	and
T4002	16177-16180	DT	denotes	all
T4003	16181-16190	VBD	denotes	exhibited
T4004	16191-16200	JJ	denotes	analogous
T4005	16201-16211	NNS	denotes	phenotypes
T4006	16211-16212	.	denotes	.
T4007	16212-16304	sentence	denotes	Mice that integrated the transgene at the single-cell stage died at or shortly after birth.
T4008	16213-16217	NNS	denotes	Mice
T4010	16218-16222	WDT	denotes	that
T4011	16223-16233	VBD	denotes	integrated
T4012	16234-16237	DT	denotes	the
T4013	16238-16247	NN	denotes	transgene
T4014	16248-16250	IN	denotes	at
T4015	16251-16254	DT	denotes	the
T4017	16255-16261	JJ	denotes	single
T4019	16261-16262	HYPH	denotes	-
T4018	16262-16266	NN	denotes	cell
T4016	16267-16272	NN	denotes	stage
T4009	16273-16277	VBD	denotes	died
T4020	16278-16280	IN	denotes	at
T4021	16281-16283	CC	denotes	or
T4022	16284-16291	RB	denotes	shortly
T4023	16292-16297	IN	denotes	after
T4024	16298-16303	NN	denotes	birth
T4025	16303-16304	.	denotes	.
T4026	16304-16461	sentence	denotes	The three surviving full-Tg founder mice harbored transgene integrations that gave stable transmission of mosaic Snail gene expression through the germline.
T4027	16305-16308	DT	denotes	The
T4029	16309-16314	CD	denotes	three
T4030	16315-16324	VBG	denotes	surviving
T4031	16325-16329	JJ	denotes	full
T4033	16329-16330	HYPH	denotes	-
T4032	16330-16332	NN	denotes	Tg
T4034	16333-16340	NN	denotes	founder
T4028	16341-16345	NNS	denotes	mice
T4035	16346-16354	VBD	denotes	harbored
T4036	16355-16364	NN	denotes	transgene
T4037	16365-16377	NNS	denotes	integrations
T4038	16378-16382	WDT	denotes	that
T4039	16383-16387	VBD	denotes	gave
T4040	16388-16394	JJ	denotes	stable
T4041	16395-16407	NN	denotes	transmission
T4042	16408-16410	IN	denotes	of
T4043	16411-16417	JJ	denotes	mosaic
T4045	16418-16423	NN	denotes	Snail
T4044	16424-16428	NN	denotes	gene
T4046	16429-16439	NN	denotes	expression
T4047	16440-16447	IN	denotes	through
T4048	16448-16451	DT	denotes	the
T4049	16452-16460	NN	denotes	germline
T4050	16460-16461	.	denotes	.
T4051	16461-16572	sentence	denotes	Progressively poor health necessitated our sacrificing most offspring from these lines within a year of birth.
T4052	16462-16475	RB	denotes	Progressively
T4053	16476-16480	JJ	denotes	poor
T4054	16481-16487	NN	denotes	health
T4055	16488-16500	VBD	denotes	necessitated
T4056	16501-16504	PRP$	denotes	our
T4057	16505-16516	VBG	denotes	sacrificing
T4058	16517-16521	JJS	denotes	most
T4059	16522-16531	NN	denotes	offspring
T4060	16532-16536	IN	denotes	from
T4061	16537-16542	DT	denotes	these
T4062	16543-16548	NNS	denotes	lines
T4063	16549-16555	IN	denotes	within
T4064	16556-16557	DT	denotes	a
T4065	16558-16562	NN	denotes	year
T4066	16563-16565	IN	denotes	of
T4067	16566-16571	NN	denotes	birth
T4068	16571-16572	.	denotes	.
T4069	16572-16686	sentence	denotes	As Snail Tg animals grew, they became distinguished by their small size, short tails, and flaky skin (Figure 2A).
T4070	16573-16575	IN	denotes	As
T4072	16576-16581	NN	denotes	Snail
T4074	16582-16584	NN	denotes	Tg
T4073	16585-16592	NNS	denotes	animals
T4071	16593-16597	VBD	denotes	grew
T4076	16597-16599	,	denotes	,
T4077	16599-16603	PRP	denotes	they
T4075	16604-16610	VBD	denotes	became
T4078	16611-16624	JJ	denotes	distinguished
T4079	16625-16627	IN	denotes	by
T4080	16628-16633	PRP$	denotes	their
T4082	16634-16639	JJ	denotes	small
T4081	16640-16644	NN	denotes	size
T4083	16644-16646	,	denotes	,
T4084	16646-16651	JJ	denotes	short
T4085	16652-16657	NNS	denotes	tails
T4086	16657-16659	,	denotes	,
T4087	16659-16662	CC	denotes	and
T4088	16663-16668	JJ	denotes	flaky
T4089	16669-16673	NN	denotes	skin
T4090	16674-16675	-LRB-	denotes	(
T4092	16675-16681	NN	denotes	Figure
T4091	16682-16684	NN	denotes	2A
T4093	16684-16685	-RRB-	denotes	)
T4094	16685-16686	.	denotes	.
T4095	16686-16797	sentence	denotes	Histological analyses of 3-d old (P3) mice revealed mosaic patches marked by epidermal thickening (Figure 2B).
T4096	16687-16699	JJ	denotes	Histological
T4097	16700-16708	NNS	denotes	analyses
T4099	16709-16711	IN	denotes	of
T4100	16712-16713	CD	denotes	3
T4102	16713-16714	HYPH	denotes	-
T4101	16714-16715	NN	denotes	d
T4103	16716-16719	JJ	denotes	old
T4105	16720-16721	-LRB-	denotes	(
T4106	16721-16723	JJ	denotes	P3
T4107	16723-16724	-RRB-	denotes	)
T4104	16725-16729	NNS	denotes	mice
T4098	16730-16738	VBD	denotes	revealed
T4108	16739-16745	NN	denotes	mosaic
T4109	16746-16753	NNS	denotes	patches
T4110	16754-16760	VBN	denotes	marked
T4111	16761-16763	IN	denotes	by
T4112	16764-16773	JJ	denotes	epidermal
T4113	16774-16784	NN	denotes	thickening
T4114	16785-16786	-LRB-	denotes	(
T4116	16786-16792	NN	denotes	Figure
T4115	16793-16795	NN	denotes	2B
T4117	16795-16796	-RRB-	denotes	)
T4118	16796-16797	.	denotes	.
T4119	16797-16952	sentence	denotes	The mosaic morphology was reflected at the level of Tg Snail protein, with only the hyperthickened regions expressing nuclear HA-tagged Snail (Figure 2C).
T4120	16798-16801	DT	denotes	The
T4122	16802-16808	NN	denotes	mosaic
T4121	16809-16819	NN	denotes	morphology
T4124	16820-16823	VBD	denotes	was
T4123	16824-16833	VBN	denotes	reflected
T4125	16834-16836	IN	denotes	at
T4126	16837-16840	DT	denotes	the
T4127	16841-16846	NN	denotes	level
T4128	16847-16849	IN	denotes	of
T4129	16850-16852	NN	denotes	Tg
T4131	16853-16858	NN	denotes	Snail
T4130	16859-16866	NN	denotes	protein
T4132	16866-16868	,	denotes	,
T4133	16868-16872	IN	denotes	with
T4135	16873-16877	RB	denotes	only
T4137	16878-16881	DT	denotes	the
T4138	16882-16896	VBN	denotes	hyperthickened
T4136	16897-16904	NNS	denotes	regions
T4134	16905-16915	VBG	denotes	expressing
T4139	16916-16923	JJ	denotes	nuclear
T4141	16924-16926	NN	denotes	HA
T4143	16926-16927	HYPH	denotes	-
T4142	16927-16933	VBN	denotes	tagged
T4140	16934-16939	NN	denotes	Snail
T4144	16940-16941	-LRB-	denotes	(
T4146	16941-16947	NN	denotes	Figure
T4145	16948-16950	NN	denotes	2C
T4147	16950-16951	-RRB-	denotes	)
T4148	16951-16952	.	denotes	.
T4149	16952-17112	sentence	denotes	These hyperthickened areas were marked by excessive proliferation, as revealed by antibodies against the proliferating nuclear antigen Ki67 (Figure 2D and 2E).
T4150	16953-16958	DT	denotes	These
T4152	16959-16973	VBN	denotes	hyperthickened
T4151	16974-16979	NNS	denotes	areas
T4154	16980-16984	VBD	denotes	were
T4153	16985-16991	VBN	denotes	marked
T4155	16992-16994	IN	denotes	by
T4156	16995-17004	JJ	denotes	excessive
T4157	17005-17018	NN	denotes	proliferation
T4158	17018-17020	,	denotes	,
T4159	17020-17022	IN	denotes	as
T4160	17023-17031	VBN	denotes	revealed
T4161	17032-17034	IN	denotes	by
T4162	17035-17045	NNS	denotes	antibodies
T4163	17046-17053	IN	denotes	against
T4164	17054-17057	DT	denotes	the
T4166	17058-17071	VBG	denotes	proliferating
T4167	17072-17079	JJ	denotes	nuclear
T4165	17080-17087	NN	denotes	antigen
T4168	17088-17092	NN	denotes	Ki67
T4169	17093-17094	-LRB-	denotes	(
T4171	17094-17100	NN	denotes	Figure
T4170	17101-17103	NN	denotes	2D
T4172	17104-17107	CC	denotes	and
T4173	17108-17110	NN	denotes	2E
T4174	17110-17111	-RRB-	denotes	)
T4175	17111-17112	.	denotes	.
T4176	17112-17325	sentence	denotes	Activated, pMAPK-positive cells were also prevalent in these areas (Figure 2F and 2G), as were cells expressing keratin 6, a keratin induced in the suprabasal layers of hyperproliferative skin (Figure 2H and 2I).
T4177	17113-17122	VBN	denotes	Activated
T4179	17122-17124	,	denotes	,
T4180	17124-17129	NN	denotes	pMAPK
T4182	17129-17130	HYPH	denotes	-
T4181	17130-17138	JJ	denotes	positive
T4178	17139-17144	NNS	denotes	cells
T4183	17145-17149	VBD	denotes	were
T4184	17150-17154	RB	denotes	also
T4185	17155-17164	JJ	denotes	prevalent
T4186	17165-17167	IN	denotes	in
T4187	17168-17173	DT	denotes	these
T4188	17174-17179	NNS	denotes	areas
T4189	17180-17181	-LRB-	denotes	(
T4191	17181-17187	NN	denotes	Figure
T4190	17188-17190	NN	denotes	2F
T4192	17191-17194	CC	denotes	and
T4193	17195-17197	NN	denotes	2G
T4194	17197-17198	-RRB-	denotes	)
T4195	17198-17200	,	denotes	,
T4196	17200-17202	IN	denotes	as
T4197	17203-17207	VBD	denotes	were
T4198	17208-17213	NNS	denotes	cells
T4199	17214-17224	VBG	denotes	expressing
T4200	17225-17232	NN	denotes	keratin
T4201	17233-17234	CD	denotes	6
T4202	17234-17236	,	denotes	,
T4203	17236-17237	DT	denotes	a
T4204	17238-17245	NN	denotes	keratin
T4205	17246-17253	VBN	denotes	induced
T4206	17254-17256	IN	denotes	in
T4207	17257-17260	DT	denotes	the
T4209	17261-17271	JJ	denotes	suprabasal
T4208	17272-17278	NNS	denotes	layers
T4210	17279-17281	IN	denotes	of
T4211	17282-17300	JJ	denotes	hyperproliferative
T4212	17301-17305	NN	denotes	skin
T4213	17306-17307	-LRB-	denotes	(
T4215	17307-17313	NN	denotes	Figure
T4214	17314-17316	NN	denotes	2H
T4216	17317-17320	CC	denotes	and
T4217	17321-17323	NN	denotes	2I
T4218	17323-17324	-RRB-	denotes	)
T4219	17324-17325	.	denotes	.
T4220	17325-19464	sentence	denotes	Figure 2 Misexpression of Snail in Mouse Skin Epidermis Results in Hyperproliferation Three different surviving Tg founder mice harbored a K14-Snail transgene that was integrated into a locus that resulted in inheritable, mosaic expression of the transgene in skin epidermis. All displayed similar abnormalities, as did their offspring. (A) P16 WT and K14-Snail Tg mice. Insets denote magnified tail segments, which displayed a mosaic, flaky appearance in Tg mice. Size differences appeared with age, and are likely due to K14-promoter activity in the tongue and oral epithelium, resulting in progressive defects and reduced food intake. Hence, skin sections from young (P3) mice were analyzed (B–I). (B) Hematoxylin- and eosin-stained Tg skin section. Double arrows demarcate the border of mosaic histology, with seemingly normal epidermis (epi) and a mature hair follicle (hf) at left and hyperthickened epidermis at right. (C) Immunofluorescence of Tg skin section labeled with antibodies as color-coded on frame. Double arrows demarcate the border of mosaic anti-Snail (green), revealing Snail expression coincident with regions of hyperthickened epidermis (at left) and absent in regions of normal epidermis (at right). (D–I) Sections of P3 WT or Tg skin (affected region) subjected to either immunofluorescence (D, E, H, and I) or immunohistochemistry (F and G) with antibodies as indicated on the panel. Anti-keratin 5 indicates K5, normally restricted to the basal layer of the epidermis; anti-keratin 6 detects keratin 6, expressed in postnatal epidermis under conditions such as wounding, in which hyperproliferation occurs. All other antibodies are as in the legend to Figure 2. Comparison of D and E provide representative examples that illustrate that pMAPK is found in only a subset of all proliferating (Ki67-positive) cells. Note also the presence of Ki67- (E) and pMAPK-positive (G) cells in some suprabasal areas; Ki67-positive cells colabeled with anti-Snail (E). Expression of the Snail transgene did not block terminal differentiation in the hyperproliferative epidermis, but it distorted it markedly (Figure 3A–3H).
T4221	19310-19320	NN	denotes	Expression
T4223	19321-19323	IN	denotes	of
T4224	19324-19327	DT	denotes	the
T4226	19328-19333	NN	denotes	Snail
T4225	19334-19343	NN	denotes	transgene
T4227	19344-19347	VBD	denotes	did
T4228	19348-19351	RB	denotes	not
T4222	19352-19357	VB	denotes	block
T4229	19358-19366	JJ	denotes	terminal
T4230	19367-19382	NN	denotes	differentiation
T4231	19383-19385	IN	denotes	in
T4232	19386-19389	DT	denotes	the
T4234	19390-19408	JJ	denotes	hyperproliferative
T4233	19409-19418	NN	denotes	epidermis
T4235	19418-19420	,	denotes	,
T4236	19420-19423	CC	denotes	but
T4237	19424-19426	PRP	denotes	it
T4238	19427-19436	VBD	denotes	distorted
T4239	19437-19439	PRP	denotes	it
T4240	19440-19448	RB	denotes	markedly
T4241	19449-19450	-LRB-	denotes	(
T4243	19450-19456	NN	denotes	Figure
T4242	19457-19459	NN	denotes	3A
T4244	19459-19460	SYM	denotes	–
T4245	19460-19462	NN	denotes	3H
T4246	19462-19463	-RRB-	denotes	)
T4247	19463-19464	.	denotes	.
T4248	19464-19601	sentence	denotes	Typical of most hyperproliferating conditions, Snail expression led to a large expansion in layers with spinous and granular morphology.
T4249	19465-19472	JJ	denotes	Typical
T4251	19473-19475	IN	denotes	of
T4252	19476-19480	JJS	denotes	most
T4254	19481-19499	NN	denotes	hyperproliferating
T4253	19500-19510	NNS	denotes	conditions
T4255	19510-19512	,	denotes	,
T4256	19512-19517	NN	denotes	Snail
T4257	19518-19528	NN	denotes	expression
T4250	19529-19532	VBD	denotes	led
T4258	19533-19535	IN	denotes	to
T4259	19536-19537	DT	denotes	a
T4261	19538-19543	JJ	denotes	large
T4260	19544-19553	NN	denotes	expansion
T4262	19554-19556	IN	denotes	in
T4263	19557-19563	NNS	denotes	layers
T4264	19564-19568	IN	denotes	with
T4265	19569-19576	JJ	denotes	spinous
T4267	19577-19580	CC	denotes	and
T4268	19581-19589	JJ	denotes	granular
T4266	19590-19600	NN	denotes	morphology
T4269	19600-19601	.	denotes	.
T4270	19601-19744	sentence	denotes	Additionally, however, was a marked and variable expansion of keratin 5 (K5), normally restricted to the innermost basal layer (see Figure 3).
T4271	19602-19614	RB	denotes	Additionally
T4273	19614-19616	,	denotes	,
T4274	19616-19623	RB	denotes	however
T4275	19623-19625	,	denotes	,
T4272	19625-19628	VBD	denotes	was
T4276	19629-19630	DT	denotes	a
T4278	19631-19637	JJ	denotes	marked
T4279	19638-19641	CC	denotes	and
T4280	19642-19650	JJ	denotes	variable
T4277	19651-19660	NN	denotes	expansion
T4281	19661-19663	IN	denotes	of
T4282	19664-19671	NN	denotes	keratin
T4283	19672-19673	CD	denotes	5
T4284	19674-19675	-LRB-	denotes	(
T4285	19675-19677	NN	denotes	K5
T4286	19677-19678	-RRB-	denotes	)
T4287	19678-19680	,	denotes	,
T4288	19680-19688	RB	denotes	normally
T4289	19689-19699	VBN	denotes	restricted
T4290	19700-19702	IN	denotes	to
T4291	19703-19706	DT	denotes	the
T4293	19707-19716	JJS	denotes	innermost
T4294	19717-19722	JJ	denotes	basal
T4292	19723-19728	NN	denotes	layer
T4295	19729-19730	-LRB-	denotes	(
T4296	19730-19733	VB	denotes	see
T4297	19734-19740	NN	denotes	Figure
T4298	19741-19742	CD	denotes	3
T4299	19742-19743	-RRB-	denotes	)
T4300	19743-19744	.	denotes	.
T4301	19744-20053	sentence	denotes	Although the failure of Snail-null mice to develop past gastrulation [21] precluded our ability to study the loss of Snail function in skin development, a good correlation emerged between the expression of Snail protein and the extension of K5, Ki67, and pMAPK suprabasally (compare data in Figures 2 and 3).
T4302	19745-19753	IN	denotes	Although
T4304	19754-19757	DT	denotes	the
T4305	19758-19765	NN	denotes	failure
T4306	19766-19768	IN	denotes	of
T4307	19769-19774	NN	denotes	Snail
T4309	19774-19775	HYPH	denotes	-
T4310	19775-19779	JJ	denotes	null
T4308	19780-19784	NNS	denotes	mice
T4311	19785-19787	TO	denotes	to
T4312	19788-19795	VB	denotes	develop
T4313	19796-19800	JJ	denotes	past
T4314	19801-19813	NN	denotes	gastrulation
T4315	19814-19815	-LRB-	denotes	[
T4316	19815-19817	CD	denotes	21
T4317	19817-19818	-RRB-	denotes	]
T4303	19819-19828	VBD	denotes	precluded
T4319	19829-19832	PRP$	denotes	our
T4320	19833-19840	NN	denotes	ability
T4321	19841-19843	TO	denotes	to
T4322	19844-19849	VB	denotes	study
T4323	19850-19853	DT	denotes	the
T4324	19854-19858	NN	denotes	loss
T4325	19859-19861	IN	denotes	of
T4326	19862-19867	NN	denotes	Snail
T4327	19868-19876	NN	denotes	function
T4328	19877-19879	IN	denotes	in
T4329	19880-19884	NN	denotes	skin
T4330	19885-19896	NN	denotes	development
T4331	19896-19898	,	denotes	,
T4332	19898-19899	DT	denotes	a
T4334	19900-19904	JJ	denotes	good
T4333	19905-19916	NN	denotes	correlation
T4318	19917-19924	VBD	denotes	emerged
T4335	19925-19932	IN	denotes	between
T4336	19933-19936	DT	denotes	the
T4337	19937-19947	NN	denotes	expression
T4338	19948-19950	IN	denotes	of
T4339	19951-19956	NN	denotes	Snail
T4340	19957-19964	NN	denotes	protein
T4341	19965-19968	CC	denotes	and
T4342	19969-19972	DT	denotes	the
T4343	19973-19982	NN	denotes	extension
T4344	19983-19985	IN	denotes	of
T4345	19986-19988	NN	denotes	K5
T4346	19988-19990	,	denotes	,
T4347	19990-19994	NN	denotes	Ki67
T4348	19994-19996	,	denotes	,
T4349	19996-19999	CC	denotes	and
T4350	20000-20005	NN	denotes	pMAPK
T4351	20006-20018	RB	denotes	suprabasally
T4352	20019-20020	-LRB-	denotes	(
T4353	20020-20027	VB	denotes	compare
T4354	20028-20032	NNS	denotes	data
T4355	20033-20035	IN	denotes	in
T4356	20036-20043	NNS	denotes	Figures
T4357	20044-20045	CD	denotes	2
T4358	20046-20049	CC	denotes	and
T4359	20050-20051	CD	denotes	3
T4360	20051-20052	-RRB-	denotes	)
T4361	20052-20053	.	denotes	.
T4362	20053-22035	sentence	denotes	Figure 3 Alterations in the Differentiation Program and Basement Membrane Organization in Snail-Expressing Tg Epidermis (A–H) Immunofluorescence of skin sections from P3 WT and Tg mice. Shown are affected areas of Tg skin; in areas where Snail protein was not expressed, stainings were normal. Sections were labeled with antibodies as indicated and color-coded on each frame. Antibodies are against markers of normal epidermal differentiation, and include K5 (a basally expressed keratin), K1 (a suprabasal keratin, expressed in spinous layer cells), involucrin (Inv; a suprabasally expressed cornified envelope protein found in upper spinous and granular layer cells), loricrin (Lor; a cornified envelope protein expressed in the granular layer), and filaggrin (Fil; a protein that bundles keratin filaments in the granular layer and stratum corneum). Note abnormal extension of anti-K5 suprabasally, often present in anti-K1 positive suprabasal Tg cells. (I–N) Immunohistochemistry (I and J) or immunofluorescence (K–N) of sections of P30 Wt (I, K, and M) and Tg (J, L, and N) (affected areas) skins using the antibodies indicated. Note that with age, affected areas of the Tg epidermis became increasingly undulating, often exhibiting papilloma-like invaginations (J). Insets in I and J are magnified views of the boxed areas, illustrating the absence (Wt) or presence (Tg) of nuclear anti-cyclin D staining. With age, affected areas of the Tg epidermis also displayed perturbations within the basement membrane, as judged by antibody labeling against either basement membrane (K and L) or hemidesmosomal (M and N) components. Double arrows in L demarcate mosaic zones, revealing that perturbations were restricted to hyperthickened, i.e., Snail-positive zones (to left of double arrows). Other abbreviations are as noted in the legend to Figure 2. The changes in hyperproliferation and differentiation were not initially accompanied by gross signs of epithelial invaginations.
T4363	21907-21910	DT	denotes	The
T4364	21911-21918	NNS	denotes	changes
T4366	21919-21921	IN	denotes	in
T4367	21922-21940	NN	denotes	hyperproliferation
T4368	21941-21944	CC	denotes	and
T4369	21945-21960	NN	denotes	differentiation
T4370	21961-21965	VBD	denotes	were
T4371	21966-21969	RB	denotes	not
T4372	21970-21979	RB	denotes	initially
T4365	21980-21991	VBN	denotes	accompanied
T4373	21992-21994	IN	denotes	by
T4374	21995-22000	JJ	denotes	gross
T4375	22001-22006	NNS	denotes	signs
T4376	22007-22009	IN	denotes	of
T4377	22010-22020	JJ	denotes	epithelial
T4378	22021-22034	NNS	denotes	invaginations
T4379	22034-22035	.	denotes	.
T4380	22035-22229	sentence	denotes	With age, however, epidermal folds and undulations developed in areas where Snail was expressed, and proliferative markers persisted in these regions (Figure 3I and 3J; anti-cyclin D staining).
T4381	22036-22040	IN	denotes	With
T4383	22041-22044	NN	denotes	age
T4384	22044-22046	,	denotes	,
T4385	22046-22053	RB	denotes	however
T4386	22053-22055	,	denotes	,
T4387	22055-22064	JJ	denotes	epidermal
T4388	22065-22070	NNS	denotes	folds
T4389	22071-22074	CC	denotes	and
T4390	22075-22086	NNS	denotes	undulations
T4382	22087-22096	VBN	denotes	developed
T4391	22097-22099	IN	denotes	in
T4392	22100-22105	NNS	denotes	areas
T4393	22106-22111	WRB	denotes	where
T4395	22112-22117	NN	denotes	Snail
T4396	22118-22121	VBD	denotes	was
T4394	22122-22131	VBN	denotes	expressed
T4397	22131-22133	,	denotes	,
T4398	22133-22136	CC	denotes	and
T4399	22137-22150	JJ	denotes	proliferative
T4400	22151-22158	NNS	denotes	markers
T4401	22159-22168	VBD	denotes	persisted
T4402	22169-22171	IN	denotes	in
T4403	22172-22177	DT	denotes	these
T4404	22178-22185	NNS	denotes	regions
T4405	22186-22187	-LRB-	denotes	(
T4407	22187-22193	NN	denotes	Figure
T4408	22194-22196	NN	denotes	3I
T4409	22197-22200	CC	denotes	and
T4410	22201-22203	NN	denotes	3J
T4411	22203-22204	:	denotes	;
T4412	22205-22216	JJ	denotes	anti-cyclin
T4413	22217-22218	NN	denotes	D
T4406	22219-22227	NN	denotes	staining
T4414	22227-22228	-RRB-	denotes	)
T4415	22228-22229	.	denotes	.
T4416	22229-22341	sentence	denotes	The undulations were accompanied by partial dissolution of the underlying basement membrane (Figure 3K and 3L).
T4417	22230-22233	DT	denotes	The
T4418	22234-22245	NNS	denotes	undulations
T4420	22246-22250	VBD	denotes	were
T4419	22251-22262	VBN	denotes	accompanied
T4421	22263-22265	IN	denotes	by
T4422	22266-22273	JJ	denotes	partial
T4423	22274-22285	NN	denotes	dissolution
T4424	22286-22288	IN	denotes	of
T4425	22289-22292	DT	denotes	the
T4427	22293-22303	JJ	denotes	underlying
T4428	22304-22312	NN	denotes	basement
T4426	22313-22321	NN	denotes	membrane
T4429	22322-22323	-LRB-	denotes	(
T4431	22323-22329	NN	denotes	Figure
T4430	22330-22332	NN	denotes	3K
T4432	22333-22336	CC	denotes	and
T4433	22337-22339	NN	denotes	3L
T4434	22339-22340	-RRB-	denotes	)
T4435	22340-22341	.	denotes	.
T4436	22341-22541	sentence	denotes	Aberrant staining was also observed with antibodies against components of the hemidesmosomes, which provide strong adhesion of basal epidermal cells to the underlying basal lamina (Figure 3M and 3N).
T4437	22342-22350	JJ	denotes	Aberrant
T4438	22351-22359	NN	denotes	staining
T4440	22360-22363	VBD	denotes	was
T4441	22364-22368	RB	denotes	also
T4439	22369-22377	VBN	denotes	observed
T4442	22378-22382	IN	denotes	with
T4443	22383-22393	NNS	denotes	antibodies
T4444	22394-22401	IN	denotes	against
T4445	22402-22412	NNS	denotes	components
T4446	22413-22415	IN	denotes	of
T4447	22416-22419	DT	denotes	the
T4448	22420-22434	NNS	denotes	hemidesmosomes
T4449	22434-22436	,	denotes	,
T4450	22436-22441	WDT	denotes	which
T4451	22442-22449	VBP	denotes	provide
T4452	22450-22456	JJ	denotes	strong
T4453	22457-22465	NN	denotes	adhesion
T4454	22466-22468	IN	denotes	of
T4455	22469-22474	JJ	denotes	basal
T4457	22475-22484	JJ	denotes	epidermal
T4456	22485-22490	NNS	denotes	cells
T4458	22491-22493	IN	denotes	to
T4459	22494-22497	DT	denotes	the
T4461	22498-22508	JJ	denotes	underlying
T4462	22509-22514	JJ	denotes	basal
T4460	22515-22521	NN	denotes	lamina
T4463	22522-22523	-LRB-	denotes	(
T4465	22523-22529	NN	denotes	Figure
T4464	22530-22532	NN	denotes	3M
T4466	22533-22536	CC	denotes	and
T4467	22537-22539	NN	denotes	3N
T4468	22539-22540	-RRB-	denotes	)
T4469	22540-22541	.	denotes	.
T4470	22541-22696	sentence	denotes	Interestingly, similar types of alterations occur in the basement membrane in the hair bud of embryonic and newborn mice when Snail is normally expressed.
T4471	22542-22555	RB	denotes	Interestingly
T4473	22555-22557	,	denotes	,
T4474	22557-22564	JJ	denotes	similar
T4475	22565-22570	NNS	denotes	types
T4476	22571-22573	IN	denotes	of
T4477	22574-22585	NNS	denotes	alterations
T4472	22586-22591	VBP	denotes	occur
T4478	22592-22594	IN	denotes	in
T4479	22595-22598	DT	denotes	the
T4481	22599-22607	NN	denotes	basement
T4480	22608-22616	NN	denotes	membrane
T4482	22617-22619	IN	denotes	in
T4483	22620-22623	DT	denotes	the
T4485	22624-22628	NN	denotes	hair
T4484	22629-22632	NN	denotes	bud
T4486	22633-22635	IN	denotes	of
T4487	22636-22645	JJ	denotes	embryonic
T4489	22646-22649	CC	denotes	and
T4490	22650-22657	JJ	denotes	newborn
T4488	22658-22662	NNS	denotes	mice
T4491	22663-22667	WRB	denotes	when
T4493	22668-22673	NN	denotes	Snail
T4494	22674-22676	VBZ	denotes	is
T4495	22677-22685	RB	denotes	normally
T4492	22686-22695	VBN	denotes	expressed
T4496	22695-22696	.	denotes	.
T4497	22696-22933	sentence	denotes	The fact that the basement membrane separating the epidermis from the dermis is altered only in the adult Tg animals suggests the involvement of intermediary factors not as readily available in the epidermis as they are in the follicle.
T4498	22697-22700	DT	denotes	The
T4499	22701-22705	NN	denotes	fact
T4501	22706-22710	IN	denotes	that
T4503	22711-22714	DT	denotes	the
T4505	22715-22723	NN	denotes	basement
T4504	22724-22732	NN	denotes	membrane
T4506	22733-22743	VBG	denotes	separating
T4507	22744-22747	DT	denotes	the
T4508	22748-22757	NN	denotes	epidermis
T4509	22758-22762	IN	denotes	from
T4510	22763-22766	DT	denotes	the
T4511	22767-22773	NN	denotes	dermis
T4512	22774-22776	VBZ	denotes	is
T4502	22777-22784	VBN	denotes	altered
T4513	22785-22789	RB	denotes	only
T4514	22790-22792	IN	denotes	in
T4515	22793-22796	DT	denotes	the
T4517	22797-22802	JJ	denotes	adult
T4518	22803-22805	NN	denotes	Tg
T4516	22806-22813	NNS	denotes	animals
T4500	22814-22822	VBZ	denotes	suggests
T4519	22823-22826	DT	denotes	the
T4520	22827-22838	NN	denotes	involvement
T4521	22839-22841	IN	denotes	of
T4522	22842-22854	JJ	denotes	intermediary
T4523	22855-22862	NNS	denotes	factors
T4524	22863-22866	RB	denotes	not
T4526	22867-22869	RB	denotes	as
T4527	22870-22877	RB	denotes	readily
T4525	22878-22887	JJ	denotes	available
T4528	22888-22890	IN	denotes	in
T4529	22891-22894	DT	denotes	the
T4530	22895-22904	NN	denotes	epidermis
T4531	22905-22907	IN	denotes	as
T4533	22908-22912	PRP	denotes	they
T4532	22913-22916	VBP	denotes	are
T4534	22917-22919	IN	denotes	in
T4535	22920-22923	DT	denotes	the
T4536	22924-22932	NN	denotes	follicle
T4537	22932-22933	.	denotes	.
T5218	22935-22943	JJ	denotes	Possible
T5219	22944-22949	NNS	denotes	Links
T5220	22950-22957	IN	denotes	between
T5221	22958-22967	JJ	denotes	Epidermal
T5222	22968-22986	NN	denotes	Hyperproliferation
T5223	22987-22990	CC	denotes	and
T5224	22991-22995	JJ	denotes	Down
T5226	22995-22996	HYPH	denotes	-
T5225	22996-23006	NN	denotes	regulation
T5227	23007-23009	IN	denotes	of
T5228	23010-23012	NN	denotes	AJ
T5229	23013-23021	NNS	denotes	Proteins
T5230	23022-23024	IN	denotes	in
T5231	23025-23030	NN	denotes	Snail
T5233	23031-23033	NN	denotes	Tg
T5232	23034-23038	NNS	denotes	Mice
T5234	23038-23358	sentence	denotes	Given that the E-cadherin promoter is a direct target for Snail-mediated repression in vitro [4,12,13], and that E-cadherin was down-regulated in Snail-expressing hair buds, we examined the status of E-cadherin and other AJ proteins within regions of hyperproliferative epidermis where Tg Snail was present (Figure 4A).
T5235	23039-23044	VBN	denotes	Given
T5237	23045-23049	IN	denotes	that
T5239	23050-23053	DT	denotes	the
T5241	23054-23055	NN	denotes	E
T5243	23055-23056	HYPH	denotes	-
T5242	23056-23064	NN	denotes	cadherin
T5240	23065-23073	NN	denotes	promoter
T5238	23074-23076	VBZ	denotes	is
T5244	23077-23078	DT	denotes	a
T5246	23079-23085	JJ	denotes	direct
T5245	23086-23092	NN	denotes	target
T5247	23093-23096	IN	denotes	for
T5248	23097-23102	NN	denotes	Snail
T5250	23102-23103	HYPH	denotes	-
T5249	23103-23111	VBN	denotes	mediated
T5251	23112-23122	NN	denotes	repression
T5252	23123-23125	FW	denotes	in
T5253	23126-23131	FW	denotes	vitro
T5254	23132-23133	-LRB-	denotes	[
T5256	23133-23134	CD	denotes	4
T5257	23134-23135	,	denotes	,
T5258	23135-23137	CD	denotes	12
T5259	23137-23138	,	denotes	,
T5255	23138-23140	CD	denotes	13
T5260	23140-23141	-RRB-	denotes	]
T5261	23141-23143	,	denotes	,
T5262	23143-23146	CC	denotes	and
T5263	23147-23151	IN	denotes	that
T5265	23152-23153	NN	denotes	E
T5267	23153-23154	HYPH	denotes	-
T5266	23154-23162	NN	denotes	cadherin
T5268	23163-23166	VBD	denotes	was
T5269	23167-23171	RB	denotes	down
T5270	23171-23172	HYPH	denotes	-
T5264	23172-23181	VBN	denotes	regulated
T5271	23182-23184	IN	denotes	in
T5272	23185-23190	NN	denotes	Snail
T5274	23190-23191	HYPH	denotes	-
T5273	23191-23201	VBG	denotes	expressing
T5276	23202-23206	NN	denotes	hair
T5275	23207-23211	NNS	denotes	buds
T5277	23211-23213	,	denotes	,
T5278	23213-23215	PRP	denotes	we
T5236	23216-23224	VBD	denotes	examined
T5279	23225-23228	DT	denotes	the
T5280	23229-23235	NN	denotes	status
T5281	23236-23238	IN	denotes	of
T5282	23239-23240	NN	denotes	E
T5284	23240-23241	HYPH	denotes	-
T5283	23241-23249	NN	denotes	cadherin
T5285	23250-23253	CC	denotes	and
T5286	23254-23259	JJ	denotes	other
T5288	23260-23262	NN	denotes	AJ
T5287	23263-23271	NN	denotes	proteins
T5289	23272-23278	IN	denotes	within
T5290	23279-23286	NNS	denotes	regions
T5291	23287-23289	IN	denotes	of
T5292	23290-23308	JJ	denotes	hyperproliferative
T5293	23309-23318	NN	denotes	epidermis
T5294	23319-23324	WRB	denotes	where
T5296	23325-23327	NN	denotes	Tg
T5297	23328-23333	NN	denotes	Snail
T5295	23334-23337	VBD	denotes	was
T5298	23338-23345	JJ	denotes	present
T5299	23346-23347	-LRB-	denotes	(
T5301	23347-23353	NN	denotes	Figure
T5300	23354-23356	NN	denotes	4A
T5302	23356-23357	-RRB-	denotes	)
T5303	23357-23358	.	denotes	.
T5304	23358-23458	sentence	denotes	In these regions, immunofluorescence staining of E-cadherin and α-catenin were markedly diminished.
T5305	23359-23361	IN	denotes	In
T5307	23362-23367	DT	denotes	these
T5308	23368-23375	NNS	denotes	regions
T5309	23375-23377	,	denotes	,
T5310	23377-23395	NN	denotes	immunofluorescence
T5311	23396-23404	NN	denotes	staining
T5312	23405-23407	IN	denotes	of
T5313	23408-23409	NN	denotes	E
T5315	23409-23410	HYPH	denotes	-
T5314	23410-23418	NN	denotes	cadherin
T5316	23419-23422	CC	denotes	and
T5317	23423-23424	NN	denotes	α
T5319	23424-23425	HYPH	denotes	-
T5318	23425-23432	NN	denotes	catenin
T5320	23433-23437	VBD	denotes	were
T5321	23438-23446	RB	denotes	markedly
T5306	23447-23457	VBN	denotes	diminished
T5322	23457-23458	.	denotes	.
T5323	23458-23572	sentence	denotes	In contrast, the intensity of antibody staining for two other AJ proteins, β-catenin and Ajuba, was still strong.
T5324	23459-23461	IN	denotes	In
T5326	23462-23470	NN	denotes	contrast
T5327	23470-23472	,	denotes	,
T5328	23472-23475	DT	denotes	the
T5329	23476-23485	NN	denotes	intensity
T5330	23486-23488	IN	denotes	of
T5331	23489-23497	NN	denotes	antibody
T5332	23498-23506	NN	denotes	staining
T5333	23507-23510	IN	denotes	for
T5334	23511-23514	CD	denotes	two
T5336	23515-23520	JJ	denotes	other
T5337	23521-23523	NN	denotes	AJ
T5335	23524-23532	NN	denotes	proteins
T5338	23532-23534	,	denotes	,
T5339	23534-23535	NN	denotes	β
T5341	23535-23536	HYPH	denotes	-
T5340	23536-23543	NN	denotes	catenin
T5342	23544-23547	CC	denotes	and
T5343	23548-23553	NN	denotes	Ajuba
T5344	23553-23555	,	denotes	,
T5325	23555-23558	VBD	denotes	was
T5345	23559-23564	RB	denotes	still
T5346	23565-23571	JJ	denotes	strong
T5347	23571-23572	.	denotes	.
T5348	23572-23760	sentence	denotes	Interestingly, however, despite appreciable immunofluorescence, localization of β-catenin and Ajuba appeared to be largely cytoplasmic rather than at cell-cell borders (Figure 4A insets).
T5349	23573-23586	RB	denotes	Interestingly
T5351	23586-23588	,	denotes	,
T5352	23588-23595	RB	denotes	however
T5353	23595-23597	,	denotes	,
T5354	23597-23604	IN	denotes	despite
T5355	23605-23616	JJ	denotes	appreciable
T5356	23617-23635	NN	denotes	immunofluorescence
T5357	23635-23637	,	denotes	,
T5358	23637-23649	NN	denotes	localization
T5359	23650-23652	IN	denotes	of
T5360	23653-23654	NN	denotes	β
T5362	23654-23655	HYPH	denotes	-
T5361	23655-23662	NN	denotes	catenin
T5363	23663-23666	CC	denotes	and
T5364	23667-23672	NN	denotes	Ajuba
T5350	23673-23681	VBD	denotes	appeared
T5365	23682-23684	TO	denotes	to
T5366	23685-23687	VB	denotes	be
T5367	23688-23695	RB	denotes	largely
T5368	23696-23707	JJ	denotes	cytoplasmic
T5369	23708-23714	RB	denotes	rather
T5370	23715-23719	IN	denotes	than
T5371	23720-23722	IN	denotes	at
T5372	23723-23727	NN	denotes	cell
T5374	23727-23728	HYPH	denotes	-
T5373	23728-23732	NN	denotes	cell
T5375	23733-23740	NNS	denotes	borders
T5376	23741-23742	-LRB-	denotes	(
T5378	23742-23748	NN	denotes	Figure
T5379	23749-23751	NN	denotes	4A
T5377	23752-23758	NNS	denotes	insets
T5380	23758-23759	-RRB-	denotes	)
T5381	23759-23760	.	denotes	.
T5382	23760-27837	sentence	denotes	Figure 4 Snail-Mediated Remodeling of AJs Contributes to Hyperproliferation (A) Immunofluorescence of skin sections from P30 Wt and Tg mice. Shown are affected areas of Tg skin; in areas where Snail protein was not expressed, stainings were normal. Antibodies used are against AJ proteins and include E-cadherin (E-cad), the transmembrane core protein; β-catenin (β-cat), which binds E-cadherin at AJs and which can also participate as a transcription cofactor when associated with LEF-1/TCF proteins in the nucleus; α-catenin (α-cat) which binds to both β-catenin and Ajuba, a close relative of zyxin; and Ajuba, which can associate with proteins that bind to the actin cytoskeleton, as well as with Grb-2, a mediator of the GTP nucleotide-exchange protein Sos, involved in activation of the Ras-MAPK signaling cascade. In Snail-expressing Tg regions, there was a reduced staining with anti-E-cad and anti-α-cat and a more diffuse staining with anti-Ajuba. Insets in the panels for β-catenin and Ajuba staining are magnified views of the boxed areas. Arrows mark membrane localization of the protein and asterisks mark cells with elevated levels of cytoplasmic β-catenin or Ajuba. (B) Western blot analyses of protein extracts from P30 Wt and Tg back and ear skins. Antibodies are as in (A) except anti-P-cad, which detects P-cadherin, whose expression in the hair follicle was not affected, and anti-tubulin, which detects tubulin, a control for equal protein loadings. Note that the reductions seen in E-cadherin and α-catenin are likely to be underestimates of the actual differences in affected regions, since the Tg skin expressed Snail mosaically. (C) In the presence of elevated Snail, α-catenin levels can be restored by overexpression of E-cadherin. Keratinocytes were transfected with either HA-tagged Snail (Snail[HA]; images on the left) or Snail(HA) and Ecad(HA) (images on the right). 2 d after transfection, cells were switched from low-calcium growth medium to high-calcium medium for 6 h to induce AJ formation. Cells were stained with antibodies as indicated on the panels. Arrowheads point to sites of intercellular contact between a Snail-transfected keratinocyte and its neighboring untransfected cell. (D) Reintroduction of E-cadherin in keratinocytes expressing Snail returns pMAPK to basal levels. Keratinocytes were transfected with control vector (K14), or Snail(HA), or Snail(HA) + E-cad(HA). After 2 d, cells were serum starved for 4 h and whole cell lysates were made and Western blotted with antibodies to pMAPK, HA to recognize the HA-tagged Snail and E-cadherin protein, 20or tubulin as a loading control. (E) Ajuba interacts with Grb-2 under conditions where α-catenin levels are reduced. Protein extracts were made from skins of P30 Wt and K14-Snail Tg P30 mice (blots on the left) and of newborn Wt and K14-Cre/α-catenin (fl/fl) conditionally null animals (blots on the right) [7]. Equal amounts of protein extracts were treated with anti-Grb-2 antibody (+) or control isotype antibody (–), and following centrifugation, immunoprecipitates were subjected to SDS-PAGE and Western blot analysis with anti-Ajuba and anti-Grb-2 antibodies. Note the presence of Ajuba only under conditions where levels of α-catenin and other AJ proteins were aberrantly low or absent. (F) Transgene expression of excess Ajuba or the Grb-2-interacting domain (pre-LIM) of Ajuba in keratinocytes results in the activation of the Ras-MAPK pathway. Primary newborn mouse keratinocytes were transfected with either the empty K14 expression vector (K14), or the expression vector driving Snail, full length Ajuba, or the pre-LIM domain of Ajuba in the absence or presence of a peptide inhibitor (inh) that disrupts the interaction between Grb-2 and Sos. 48 h posttransfection, protein extracts were prepared and subjected to SDS-PAGE and Western blot analyses with antibodies against pMAPK, total MAPK, Ajuba (also recognizing the smaller, pre-LIM domain), and Snail. Architectural differences in the epidermis made Western blot analyses somewhat difficult to gauge.
T5383	27739-27752	JJ	denotes	Architectural
T5384	27753-27764	NNS	denotes	differences
T5386	27765-27767	IN	denotes	in
T5387	27768-27771	DT	denotes	the
T5388	27772-27781	NN	denotes	epidermis
T5385	27782-27786	VBD	denotes	made
T5389	27787-27794	NNP	denotes	Western
T5390	27795-27799	NN	denotes	blot
T5391	27800-27808	NNS	denotes	analyses
T5393	27809-27817	RB	denotes	somewhat
T5392	27818-27827	JJ	denotes	difficult
T5394	27828-27830	TO	denotes	to
T5395	27831-27836	VB	denotes	gauge
T5396	27836-27837	.	denotes	.
T5397	27837-27963	sentence	denotes	However, in regions such as ear skin, where the highest levels of Snail protein were expressed, the effects were accentuated.
T5398	27838-27845	RB	denotes	However
T5400	27845-27847	,	denotes	,
T5401	27847-27849	IN	denotes	in
T5402	27850-27857	NNS	denotes	regions
T5403	27858-27862	JJ	denotes	such
T5404	27863-27865	IN	denotes	as
T5405	27866-27869	NN	denotes	ear
T5406	27870-27874	NN	denotes	skin
T5407	27874-27876	,	denotes	,
T5408	27876-27881	WRB	denotes	where
T5410	27882-27885	DT	denotes	the
T5412	27886-27893	JJS	denotes	highest
T5411	27894-27900	NNS	denotes	levels
T5413	27901-27903	IN	denotes	of
T5414	27904-27909	NN	denotes	Snail
T5415	27910-27917	NN	denotes	protein
T5416	27918-27922	VBD	denotes	were
T5409	27923-27932	VBN	denotes	expressed
T5417	27932-27934	,	denotes	,
T5418	27934-27937	DT	denotes	the
T5419	27938-27945	NNS	denotes	effects
T5420	27946-27950	VBD	denotes	were
T5399	27951-27962	VBN	denotes	accentuated
T5421	27962-27963	.	denotes	.
T5422	27963-28155	sentence	denotes	In both back skin and ear skin, overall levels of E-cadherin and α-catenin were reduced, under conditions where β-catenin and Ajuba levels remained unchanged relative to controls (Figure 4B).
T5423	27964-27966	IN	denotes	In
T5425	27967-27971	CC	denotes	both
T5427	27972-27976	NN	denotes	back
T5426	27977-27981	NN	denotes	skin
T5428	27982-27985	CC	denotes	and
T5429	27986-27989	NN	denotes	ear
T5430	27990-27994	NN	denotes	skin
T5431	27994-27996	,	denotes	,
T5432	27996-28003	JJ	denotes	overall
T5433	28004-28010	NNS	denotes	levels
T5434	28011-28013	IN	denotes	of
T5435	28014-28015	NN	denotes	E
T5437	28015-28016	HYPH	denotes	-
T5436	28016-28024	NN	denotes	cadherin
T5438	28025-28028	CC	denotes	and
T5439	28029-28030	NN	denotes	α
T5441	28030-28031	HYPH	denotes	-
T5440	28031-28038	NN	denotes	catenin
T5442	28039-28043	VBD	denotes	were
T5424	28044-28051	VBN	denotes	reduced
T5443	28051-28053	,	denotes	,
T5444	28053-28058	IN	denotes	under
T5445	28059-28069	NNS	denotes	conditions
T5446	28070-28075	WRB	denotes	where
T5448	28076-28077	NN	denotes	β
T5450	28077-28078	HYPH	denotes	-
T5449	28078-28085	NN	denotes	catenin
T5452	28086-28089	CC	denotes	and
T5453	28090-28095	NN	denotes	Ajuba
T5451	28096-28102	NNS	denotes	levels
T5447	28103-28111	VBD	denotes	remained
T5454	28112-28121	JJ	denotes	unchanged
T5455	28122-28130	JJ	denotes	relative
T5456	28131-28133	IN	denotes	to
T5457	28134-28142	NNS	denotes	controls
T5458	28143-28144	-LRB-	denotes	(
T5460	28144-28150	NN	denotes	Figure
T5459	28151-28153	NN	denotes	4B
T5461	28153-28154	-RRB-	denotes	)
T5462	28154-28155	.	denotes	.
T5463	28155-28260	sentence	denotes	Taken together, these data were consistent with our results obtained from immunofluorescence microscopy.
T5464	28156-28161	VBN	denotes	Taken
T5466	28162-28170	RB	denotes	together
T5467	28170-28172	,	denotes	,
T5468	28172-28177	DT	denotes	these
T5469	28178-28182	NNS	denotes	data
T5465	28183-28187	VBD	denotes	were
T5470	28188-28198	JJ	denotes	consistent
T5471	28199-28203	IN	denotes	with
T5472	28204-28207	PRP$	denotes	our
T5473	28208-28215	NNS	denotes	results
T5474	28216-28224	VBN	denotes	obtained
T5475	28225-28229	IN	denotes	from
T5476	28230-28248	NN	denotes	immunofluorescence
T5477	28249-28259	NN	denotes	microscopy
T5478	28259-28260	.	denotes	.
T5479	28260-28456	sentence	denotes	A priori, the decrease in α-catenin levels could be due to either direct transcriptional repression by Snail or perturbations in AJ formation caused by the decrease in E-cadherin gene expression.
T5480	28261-28262	FW	denotes	A
T5481	28263-28269	FW	denotes	priori
T5483	28269-28271	,	denotes	,
T5484	28271-28274	DT	denotes	the
T5485	28275-28283	NN	denotes	decrease
T5486	28284-28286	IN	denotes	in
T5487	28287-28288	NN	denotes	α
T5489	28288-28289	HYPH	denotes	-
T5488	28289-28296	NN	denotes	catenin
T5490	28297-28303	NNS	denotes	levels
T5491	28304-28309	MD	denotes	could
T5482	28310-28312	VB	denotes	be
T5492	28313-28316	IN	denotes	due
T5493	28317-28319	IN	denotes	to
T5494	28320-28326	CC	denotes	either
T5496	28327-28333	JJ	denotes	direct
T5497	28334-28349	JJ	denotes	transcriptional
T5495	28350-28360	NN	denotes	repression
T5498	28361-28363	IN	denotes	by
T5499	28364-28369	NN	denotes	Snail
T5500	28370-28372	CC	denotes	or
T5501	28373-28386	NNS	denotes	perturbations
T5502	28387-28389	IN	denotes	in
T5503	28390-28392	NN	denotes	AJ
T5504	28393-28402	NN	denotes	formation
T5505	28403-28409	VBN	denotes	caused
T5506	28410-28412	IN	denotes	by
T5507	28413-28416	DT	denotes	the
T5508	28417-28425	NN	denotes	decrease
T5509	28426-28428	IN	denotes	in
T5510	28429-28430	NN	denotes	E
T5512	28430-28431	HYPH	denotes	-
T5511	28431-28439	NN	denotes	cadherin
T5514	28440-28444	NN	denotes	gene
T5513	28445-28455	NN	denotes	expression
T5515	28455-28456	.	denotes	.
T5516	28456-28626	sentence	denotes	To distinguish between these possibilities, we tested whether α-catenin levels could be restored by exogenous expression of E-cadherin in Snail-expressing keratinocytes.
T5517	28457-28459	TO	denotes	To
T5518	28460-28471	VB	denotes	distinguish
T5520	28472-28479	IN	denotes	between
T5521	28480-28485	DT	denotes	these
T5522	28486-28499	NNS	denotes	possibilities
T5523	28499-28501	,	denotes	,
T5524	28501-28503	PRP	denotes	we
T5519	28504-28510	VBD	denotes	tested
T5525	28511-28518	IN	denotes	whether
T5527	28519-28520	NN	denotes	α
T5529	28520-28521	HYPH	denotes	-
T5528	28521-28528	NN	denotes	catenin
T5530	28529-28535	NNS	denotes	levels
T5531	28536-28541	MD	denotes	could
T5532	28542-28544	VB	denotes	be
T5526	28545-28553	VBN	denotes	restored
T5533	28554-28556	IN	denotes	by
T5534	28557-28566	JJ	denotes	exogenous
T5535	28567-28577	NN	denotes	expression
T5536	28578-28580	IN	denotes	of
T5537	28581-28582	NN	denotes	E
T5539	28582-28583	HYPH	denotes	-
T5538	28583-28591	NN	denotes	cadherin
T5540	28592-28594	IN	denotes	in
T5541	28595-28600	NN	denotes	Snail
T5543	28600-28601	HYPH	denotes	-
T5542	28601-28611	VBG	denotes	expressing
T5544	28612-28625	NNS	denotes	keratinocytes
T5545	28625-28626	.	denotes	.
T5546	28626-28781	sentence	denotes	As shown in Figure 4C, transiently transfected keratinocytes expressing HA-tagged Snail displayed a loss of E-cadherin and α-catenin at cell-cell borders.
T5547	28627-28629	IN	denotes	As
T5548	28630-28635	VBN	denotes	shown
T5550	28636-28638	IN	denotes	in
T5551	28639-28645	NN	denotes	Figure
T5552	28646-28648	NN	denotes	4C
T5553	28648-28650	,	denotes	,
T5554	28650-28661	RB	denotes	transiently
T5555	28662-28673	VBN	denotes	transfected
T5556	28674-28687	NNS	denotes	keratinocytes
T5557	28688-28698	VBG	denotes	expressing
T5558	28699-28701	NN	denotes	HA
T5560	28701-28702	HYPH	denotes	-
T5559	28702-28708	VBN	denotes	tagged
T5561	28709-28714	NN	denotes	Snail
T5549	28715-28724	VBD	denotes	displayed
T5562	28725-28726	DT	denotes	a
T5563	28727-28731	NN	denotes	loss
T5564	28732-28734	IN	denotes	of
T5565	28735-28736	NN	denotes	E
T5567	28736-28737	HYPH	denotes	-
T5566	28737-28745	NN	denotes	cadherin
T5568	28746-28749	CC	denotes	and
T5569	28750-28751	NN	denotes	α
T5571	28751-28752	HYPH	denotes	-
T5570	28752-28759	NN	denotes	catenin
T5572	28760-28762	IN	denotes	at
T5573	28763-28767	NN	denotes	cell
T5575	28767-28768	HYPH	denotes	-
T5574	28768-28772	NN	denotes	cell
T5576	28773-28780	NNS	denotes	borders
T5577	28780-28781	.	denotes	.
T5578	28781-28971	sentence	denotes	Coexpression of exogenous HA-tagged E-cadherin not only enabled cell-cell border localization of E-cadherin protein, but also rescued the cell-cell border staining of α-catenin (Figure 4C).
T5579	28782-28794	NN	denotes	Coexpression
T5581	28795-28797	IN	denotes	of
T5582	28798-28807	JJ	denotes	exogenous
T5584	28808-28810	NN	denotes	HA
T5586	28810-28811	HYPH	denotes	-
T5585	28811-28817	VBN	denotes	tagged
T5587	28818-28819	NN	denotes	E
T5588	28819-28820	HYPH	denotes	-
T5583	28820-28828	NN	denotes	cadherin
T5589	28829-28832	RB	denotes	not
T5590	28833-28837	RB	denotes	only
T5580	28838-28845	VBD	denotes	enabled
T5591	28846-28850	NN	denotes	cell
T5593	28850-28851	HYPH	denotes	-
T5592	28851-28855	NN	denotes	cell
T5595	28856-28862	NN	denotes	border
T5594	28863-28875	NN	denotes	localization
T5596	28876-28878	IN	denotes	of
T5597	28879-28880	NN	denotes	E
T5599	28880-28881	HYPH	denotes	-
T5598	28881-28889	NN	denotes	cadherin
T5600	28890-28897	NN	denotes	protein
T5601	28897-28899	,	denotes	,
T5602	28899-28902	CC	denotes	but
T5603	28903-28907	RB	denotes	also
T5604	28908-28915	VBD	denotes	rescued
T5605	28916-28919	DT	denotes	the
T5607	28920-28924	NN	denotes	cell
T5609	28924-28925	HYPH	denotes	-
T5608	28925-28929	NN	denotes	cell
T5610	28930-28936	NN	denotes	border
T5606	28937-28945	NN	denotes	staining
T5611	28946-28948	IN	denotes	of
T5612	28949-28950	NN	denotes	α
T5614	28950-28951	HYPH	denotes	-
T5613	28951-28958	NN	denotes	catenin
T5615	28959-28960	-LRB-	denotes	(
T5617	28960-28966	NN	denotes	Figure
T5616	28967-28969	NN	denotes	4C
T5618	28969-28970	-RRB-	denotes	)
T5619	28970-28971	.	denotes	.
T5620	28971-29131	sentence	denotes	The ability to restore α-catenin expression and localization under these conditions argues against the notion that Snail transcriptionally represses α-catenin.
T5621	28972-28975	DT	denotes	The
T5622	28976-28983	NN	denotes	ability
T5624	28984-28986	TO	denotes	to
T5625	28987-28994	VB	denotes	restore
T5626	28995-28996	NN	denotes	α
T5628	28996-28997	HYPH	denotes	-
T5627	28997-29004	NN	denotes	catenin
T5629	29005-29015	NN	denotes	expression
T5630	29016-29019	CC	denotes	and
T5631	29020-29032	NN	denotes	localization
T5632	29033-29038	IN	denotes	under
T5633	29039-29044	DT	denotes	these
T5634	29045-29055	NNS	denotes	conditions
T5623	29056-29062	VBZ	denotes	argues
T5635	29063-29070	IN	denotes	against
T5636	29071-29074	DT	denotes	the
T5637	29075-29081	NN	denotes	notion
T5638	29082-29086	IN	denotes	that
T5640	29087-29092	NN	denotes	Snail
T5641	29093-29110	RB	denotes	transcriptionally
T5639	29111-29120	VBZ	denotes	represses
T5642	29121-29122	NN	denotes	α
T5644	29122-29123	HYPH	denotes	-
T5643	29123-29130	NN	denotes	catenin
T5645	29130-29131	.	denotes	.
T5646	29131-29262	sentence	denotes	Rather, the findings are consistent with a previous report that E-cadherin is required for the translation of α-catenin mRNA [22].
T5647	29132-29138	RB	denotes	Rather
T5649	29138-29140	,	denotes	,
T5650	29140-29143	DT	denotes	the
T5651	29144-29152	NNS	denotes	findings
T5648	29153-29156	VBP	denotes	are
T5652	29157-29167	JJ	denotes	consistent
T5653	29168-29172	IN	denotes	with
T5654	29173-29174	DT	denotes	a
T5656	29175-29183	JJ	denotes	previous
T5655	29184-29190	NN	denotes	report
T5657	29191-29195	IN	denotes	that
T5659	29196-29197	NN	denotes	E
T5661	29197-29198	HYPH	denotes	-
T5660	29198-29206	NN	denotes	cadherin
T5662	29207-29209	VBZ	denotes	is
T5658	29210-29218	VBN	denotes	required
T5663	29219-29222	IN	denotes	for
T5664	29223-29226	DT	denotes	the
T5665	29227-29238	NN	denotes	translation
T5666	29239-29241	IN	denotes	of
T5667	29242-29243	NN	denotes	α
T5669	29243-29244	HYPH	denotes	-
T5668	29244-29251	NN	denotes	catenin
T5670	29252-29256	NN	denotes	mRNA
T5671	29257-29258	-LRB-	denotes	[
T5672	29258-29260	CD	denotes	22
T5673	29260-29261	-RRB-	denotes	]
T5674	29261-29262	.	denotes	.
T5675	29262-29453	sentence	denotes	Despite the reductions in AJ markers, Tg skin still displayed sealed membranes and intercellular junctions that were largely intact, as judged by ultrastructural analyses (unpublished data).
T5676	29263-29270	IN	denotes	Despite
T5678	29271-29274	DT	denotes	the
T5679	29275-29285	NNS	denotes	reductions
T5680	29286-29288	IN	denotes	in
T5681	29289-29291	NN	denotes	AJ
T5682	29292-29299	NNS	denotes	markers
T5683	29299-29301	,	denotes	,
T5684	29301-29303	NN	denotes	Tg
T5685	29304-29308	NN	denotes	skin
T5686	29309-29314	RB	denotes	still
T5677	29315-29324	VBD	denotes	displayed
T5687	29325-29331	JJ	denotes	sealed
T5688	29332-29341	NNS	denotes	membranes
T5689	29342-29345	CC	denotes	and
T5690	29346-29359	JJ	denotes	intercellular
T5691	29360-29369	NNS	denotes	junctions
T5692	29370-29374	WDT	denotes	that
T5693	29375-29379	VBD	denotes	were
T5694	29380-29387	RB	denotes	largely
T5695	29388-29394	JJ	denotes	intact
T5696	29394-29396	,	denotes	,
T5697	29396-29398	IN	denotes	as
T5698	29399-29405	VBN	denotes	judged
T5699	29406-29408	IN	denotes	by
T5700	29409-29424	JJ	denotes	ultrastructural
T5701	29425-29433	NNS	denotes	analyses
T5702	29434-29435	-LRB-	denotes	(
T5704	29435-29446	JJ	denotes	unpublished
T5703	29447-29451	NNS	denotes	data
T5705	29451-29452	-RRB-	denotes	)
T5706	29452-29453	.	denotes	.
T5707	29453-29651	sentence	denotes	In this respect, the skin epithelium resembled that of the hair bud, where the down-regulation in junction proteins is permissive for cell-cell remodeling without abrogating intercellular adhesion.
T5708	29454-29456	IN	denotes	In
T5710	29457-29461	DT	denotes	this
T5711	29462-29469	NN	denotes	respect
T5712	29469-29471	,	denotes	,
T5713	29471-29474	DT	denotes	the
T5715	29475-29479	NN	denotes	skin
T5714	29480-29490	NN	denotes	epithelium
T5709	29491-29500	VBD	denotes	resembled
T5716	29501-29505	DT	denotes	that
T5717	29506-29508	IN	denotes	of
T5718	29509-29512	DT	denotes	the
T5720	29513-29517	NN	denotes	hair
T5719	29518-29521	NN	denotes	bud
T5721	29521-29523	,	denotes	,
T5722	29523-29528	WRB	denotes	where
T5724	29529-29532	DT	denotes	the
T5726	29533-29537	JJ	denotes	down
T5727	29537-29538	HYPH	denotes	-
T5725	29538-29548	NN	denotes	regulation
T5728	29549-29551	IN	denotes	in
T5729	29552-29560	NN	denotes	junction
T5730	29561-29569	NN	denotes	proteins
T5723	29570-29572	VBZ	denotes	is
T5731	29573-29583	JJ	denotes	permissive
T5732	29584-29587	IN	denotes	for
T5733	29588-29592	NN	denotes	cell
T5735	29592-29593	HYPH	denotes	-
T5734	29593-29597	NN	denotes	cell
T5736	29598-29608	NN	denotes	remodeling
T5737	29609-29616	IN	denotes	without
T5738	29617-29627	VBG	denotes	abrogating
T5739	29628-29641	JJ	denotes	intercellular
T5740	29642-29650	NN	denotes	adhesion
T5741	29650-29651	.	denotes	.
T5742	29651-29851	sentence	denotes	The similarities between Snail Tg epidermis and hair buds extended to the hyperproliferative state, leading us to wonder whether the down-regulation of AJ proteins might contribute to this condition.
T5743	29652-29655	DT	denotes	The
T5744	29656-29668	NNS	denotes	similarities
T5746	29669-29676	IN	denotes	between
T5747	29677-29682	NN	denotes	Snail
T5749	29683-29685	NN	denotes	Tg
T5748	29686-29695	NN	denotes	epidermis
T5750	29696-29699	CC	denotes	and
T5751	29700-29704	NN	denotes	hair
T5752	29705-29709	NNS	denotes	buds
T5745	29710-29718	VBD	denotes	extended
T5753	29719-29721	IN	denotes	to
T5754	29722-29725	DT	denotes	the
T5756	29726-29744	JJ	denotes	hyperproliferative
T5755	29745-29750	NN	denotes	state
T5757	29750-29752	,	denotes	,
T5758	29752-29759	VBG	denotes	leading
T5759	29760-29762	PRP	denotes	us
T5760	29763-29765	TO	denotes	to
T5761	29766-29772	VB	denotes	wonder
T5762	29773-29780	IN	denotes	whether
T5764	29781-29784	DT	denotes	the
T5766	29785-29789	JJ	denotes	down
T5767	29789-29790	HYPH	denotes	-
T5765	29790-29800	NN	denotes	regulation
T5768	29801-29803	IN	denotes	of
T5769	29804-29806	NN	denotes	AJ
T5770	29807-29815	NN	denotes	proteins
T5771	29816-29821	MD	denotes	might
T5763	29822-29832	VB	denotes	contribute
T5772	29833-29835	IN	denotes	to
T5773	29836-29840	DT	denotes	this
T5774	29841-29850	NN	denotes	condition
T5775	29850-29851	.	denotes	.
T5776	29851-30061	sentence	denotes	Given the increase in pMAPK staining in Snail Tg epidermis (see Figure 2G), we used pMAPK levels as our assay to test whether the loss of E-cadherin contributed to the Snail-mediated increase in proliferation.
T5777	29852-29857	VBN	denotes	Given
T5779	29858-29861	DT	denotes	the
T5780	29862-29870	NN	denotes	increase
T5781	29871-29873	IN	denotes	in
T5782	29874-29879	NN	denotes	pMAPK
T5783	29880-29888	NN	denotes	staining
T5784	29889-29891	IN	denotes	in
T5785	29892-29897	NN	denotes	Snail
T5787	29898-29900	NN	denotes	Tg
T5786	29901-29910	NN	denotes	epidermis
T5788	29911-29912	-LRB-	denotes	(
T5789	29912-29915	VB	denotes	see
T5790	29916-29922	NN	denotes	Figure
T5791	29923-29925	NN	denotes	2G
T5792	29925-29926	-RRB-	denotes	)
T5793	29926-29928	,	denotes	,
T5794	29928-29930	PRP	denotes	we
T5778	29931-29935	VBD	denotes	used
T5795	29936-29941	NN	denotes	pMAPK
T5796	29942-29948	NNS	denotes	levels
T5797	29949-29951	IN	denotes	as
T5798	29952-29955	PRP$	denotes	our
T5799	29956-29961	NN	denotes	assay
T5800	29962-29964	TO	denotes	to
T5801	29965-29969	VB	denotes	test
T5802	29970-29977	IN	denotes	whether
T5804	29978-29981	DT	denotes	the
T5805	29982-29986	NN	denotes	loss
T5806	29987-29989	IN	denotes	of
T5807	29990-29991	NN	denotes	E
T5809	29991-29992	HYPH	denotes	-
T5808	29992-30000	NN	denotes	cadherin
T5803	30001-30012	VBD	denotes	contributed
T5810	30013-30015	IN	denotes	to
T5811	30016-30019	DT	denotes	the
T5813	30020-30025	NN	denotes	Snail
T5815	30025-30026	HYPH	denotes	-
T5814	30026-30034	VBN	denotes	mediated
T5812	30035-30043	NN	denotes	increase
T5816	30044-30046	IN	denotes	in
T5817	30047-30060	NN	denotes	proliferation
T5818	30060-30061	.	denotes	.
T5819	30061-30234	sentence	denotes	Consistent with our in vivo observations, transfected keratinocytes expressing Snail exhibited a substantial increase in pMAPK levels relative to control cells (Figure 4D).
T5820	30062-30072	JJ	denotes	Consistent
T5822	30073-30077	IN	denotes	with
T5823	30078-30081	PRP$	denotes	our
T5825	30082-30084	FW	denotes	in
T5826	30085-30089	FW	denotes	vivo
T5824	30090-30102	NNS	denotes	observations
T5827	30102-30104	,	denotes	,
T5828	30104-30115	VBN	denotes	transfected
T5829	30116-30129	NNS	denotes	keratinocytes
T5830	30130-30140	VBG	denotes	expressing
T5831	30141-30146	NN	denotes	Snail
T5821	30147-30156	VBD	denotes	exhibited
T5832	30157-30158	DT	denotes	a
T5834	30159-30170	JJ	denotes	substantial
T5833	30171-30179	NN	denotes	increase
T5835	30180-30182	IN	denotes	in
T5836	30183-30188	NN	denotes	pMAPK
T5837	30189-30195	NNS	denotes	levels
T5838	30196-30204	JJ	denotes	relative
T5839	30205-30207	IN	denotes	to
T5840	30208-30215	NN	denotes	control
T5841	30216-30221	NNS	denotes	cells
T5842	30222-30223	-LRB-	denotes	(
T5844	30223-30229	NN	denotes	Figure
T5843	30230-30232	NN	denotes	4D
T5845	30232-30233	-RRB-	denotes	)
T5846	30233-30234	.	denotes	.
T5847	30234-30306	sentence	denotes	Coexpression of E-cadherin with Snail appeared to abrogate this effect.
T5848	30235-30247	NN	denotes	Coexpression
T5850	30248-30250	IN	denotes	of
T5851	30251-30252	NN	denotes	E
T5853	30252-30253	HYPH	denotes	-
T5852	30253-30261	NN	denotes	cadherin
T5854	30262-30266	IN	denotes	with
T5855	30267-30272	NN	denotes	Snail
T5849	30273-30281	VBD	denotes	appeared
T5856	30282-30284	TO	denotes	to
T5857	30285-30293	VB	denotes	abrogate
T5858	30294-30298	DT	denotes	this
T5859	30299-30305	NN	denotes	effect
T5860	30305-30306	.	denotes	.
T5861	30306-30517	sentence	denotes	Together, these findings raised the possibility that an AJ-associated protein that is normally sequestered at the plasma membrane may participate in a proliferation signaling pathway when AJs are deconstructed.
T5862	30307-30315	RB	denotes	Together
T5864	30315-30317	,	denotes	,
T5865	30317-30322	DT	denotes	these
T5866	30323-30331	NNS	denotes	findings
T5863	30332-30338	VBD	denotes	raised
T5867	30339-30342	DT	denotes	the
T5868	30343-30354	NN	denotes	possibility
T5869	30355-30359	IN	denotes	that
T5871	30360-30362	DT	denotes	an
T5873	30363-30365	NN	denotes	AJ
T5875	30365-30366	HYPH	denotes	-
T5874	30366-30376	VBN	denotes	associated
T5872	30377-30384	NN	denotes	protein
T5876	30385-30389	WDT	denotes	that
T5878	30390-30392	VBZ	denotes	is
T5879	30393-30401	RB	denotes	normally
T5877	30402-30413	VBN	denotes	sequestered
T5880	30414-30416	IN	denotes	at
T5881	30417-30420	DT	denotes	the
T5883	30421-30427	NN	denotes	plasma
T5882	30428-30436	NN	denotes	membrane
T5884	30437-30440	MD	denotes	may
T5870	30441-30452	VB	denotes	participate
T5885	30453-30455	IN	denotes	in
T5886	30456-30457	DT	denotes	a
T5888	30458-30471	NN	denotes	proliferation
T5889	30472-30481	NN	denotes	signaling
T5887	30482-30489	NN	denotes	pathway
T5890	30490-30494	WRB	denotes	when
T5892	30495-30498	NNS	denotes	AJs
T5893	30499-30502	VBP	denotes	are
T5891	30503-30516	VBN	denotes	deconstructed
T5894	30516-30517	.	denotes	.
T5895	30517-30757	sentence	denotes	Numerous studies have correlated a down-regulation of E-cadherin with a translocation of β-catenin to the nucleus and a transactivation of genes that are regulated by the LEF-1/T cell factor (TCF) family of DNA binding proteins [23,24,25].
T5896	30518-30526	JJ	denotes	Numerous
T5897	30527-30534	NNS	denotes	studies
T5899	30535-30539	VBP	denotes	have
T5898	30540-30550	VBN	denotes	correlated
T5900	30551-30552	DT	denotes	a
T5902	30553-30557	JJ	denotes	down
T5903	30557-30558	HYPH	denotes	-
T5901	30558-30568	NN	denotes	regulation
T5904	30569-30571	IN	denotes	of
T5905	30572-30573	NN	denotes	E
T5907	30573-30574	HYPH	denotes	-
T5906	30574-30582	NN	denotes	cadherin
T5908	30583-30587	IN	denotes	with
T5909	30588-30589	DT	denotes	a
T5910	30590-30603	NN	denotes	translocation
T5911	30604-30606	IN	denotes	of
T5912	30607-30608	NN	denotes	β
T5914	30608-30609	HYPH	denotes	-
T5913	30609-30616	NN	denotes	catenin
T5915	30617-30619	IN	denotes	to
T5916	30620-30623	DT	denotes	the
T5917	30624-30631	NN	denotes	nucleus
T5918	30632-30635	CC	denotes	and
T5919	30636-30637	DT	denotes	a
T5920	30638-30653	NN	denotes	transactivation
T5921	30654-30656	IN	denotes	of
T5922	30657-30662	NNS	denotes	genes
T5923	30663-30667	WDT	denotes	that
T5925	30668-30671	VBP	denotes	are
T5924	30672-30681	VBN	denotes	regulated
T5926	30682-30684	IN	denotes	by
T5927	30685-30688	DT	denotes	the
T5929	30689-30692	NN	denotes	LEF
T5930	30692-30693	HYPH	denotes	-
T5931	30693-30694	CD	denotes	1
T5932	30694-30695	HYPH	denotes	/
T5933	30695-30696	NN	denotes	T
T5934	30697-30701	NN	denotes	cell
T5935	30702-30708	NN	denotes	factor
T5936	30709-30710	-LRB-	denotes	(
T5937	30710-30713	NN	denotes	TCF
T5938	30713-30714	-RRB-	denotes	)
T5928	30715-30721	NN	denotes	family
T5939	30722-30724	IN	denotes	of
T5940	30725-30728	NN	denotes	DNA
T5941	30729-30736	VBG	denotes	binding
T5942	30737-30745	NN	denotes	proteins
T5943	30746-30747	-LRB-	denotes	[
T5945	30747-30749	CD	denotes	23
T5946	30749-30750	,	denotes	,
T5947	30750-30752	CD	denotes	24
T5948	30752-30753	,	denotes	,
T5944	30753-30755	CD	denotes	25
T5949	30755-30756	-RRB-	denotes	]
T5950	30756-30757	.	denotes	.
T5951	30757-30963	sentence	denotes	The presence of nuclear cyclin D in hyperproliferative Snail Tg epidermis was particularly intriguing since prior studies have reported cyclin D gene as a direct target of TCF/β-catenin transcription [26].
T5952	30758-30761	DT	denotes	The
T5953	30762-30770	NN	denotes	presence
T5955	30771-30773	IN	denotes	of
T5956	30774-30781	JJ	denotes	nuclear
T5958	30782-30788	NN	denotes	cyclin
T5957	30789-30790	NN	denotes	D
T5959	30791-30793	IN	denotes	in
T5960	30794-30812	JJ	denotes	hyperproliferative
T5962	30813-30818	NN	denotes	Snail
T5963	30819-30821	NN	denotes	Tg
T5961	30822-30831	NN	denotes	epidermis
T5954	30832-30835	VBD	denotes	was
T5964	30836-30848	RB	denotes	particularly
T5965	30849-30859	JJ	denotes	intriguing
T5966	30860-30865	IN	denotes	since
T5968	30866-30871	JJ	denotes	prior
T5969	30872-30879	NNS	denotes	studies
T5970	30880-30884	VBP	denotes	have
T5967	30885-30893	VBN	denotes	reported
T5971	30894-30900	NN	denotes	cyclin
T5972	30901-30902	NN	denotes	D
T5973	30903-30907	NN	denotes	gene
T5974	30908-30910	IN	denotes	as
T5975	30911-30912	DT	denotes	a
T5977	30913-30919	JJ	denotes	direct
T5976	30920-30926	NN	denotes	target
T5978	30927-30929	IN	denotes	of
T5979	30930-30933	NN	denotes	TCF
T5981	30933-30934	HYPH	denotes	/
T5980	30934-30935	NN	denotes	β
T5983	30935-30936	HYPH	denotes	-
T5984	30936-30943	NN	denotes	catenin
T5982	30944-30957	NN	denotes	transcription
T5985	30958-30959	-LRB-	denotes	[
T5986	30959-30961	CD	denotes	26
T5987	30961-30962	-RRB-	denotes	]
T5988	30962-30963	.	denotes	.
T5989	30963-31173	sentence	denotes	This said, we did not detect nuclear β-catenin in our Tg epidermis, and mating the Snail Tg mice against the TOPGal reporter mouse [20] gave no signs of ectopic LEF-1/Tcf/β-catenin activity (unpublished data).
T5990	30964-30968	DT	denotes	This
T5991	30969-30973	VBN	denotes	said
T5993	30973-30975	,	denotes	,
T5994	30975-30977	PRP	denotes	we
T5995	30978-30981	VBD	denotes	did
T5996	30982-30985	RB	denotes	not
T5992	30986-30992	VB	denotes	detect
T5997	30993-31000	JJ	denotes	nuclear
T5999	31001-31002	NN	denotes	β
T6000	31002-31003	HYPH	denotes	-
T5998	31003-31010	NN	denotes	catenin
T6001	31011-31013	IN	denotes	in
T6002	31014-31017	PRP$	denotes	our
T6004	31018-31020	NN	denotes	Tg
T6003	31021-31030	NN	denotes	epidermis
T6005	31030-31032	,	denotes	,
T6006	31032-31035	CC	denotes	and
T6007	31036-31042	VBG	denotes	mating
T6009	31043-31046	DT	denotes	the
T6011	31047-31052	NN	denotes	Snail
T6012	31053-31055	NN	denotes	Tg
T6010	31056-31060	NNS	denotes	mice
T6013	31061-31068	IN	denotes	against
T6014	31069-31072	DT	denotes	the
T6016	31073-31079	NN	denotes	TOPGal
T6017	31080-31088	NN	denotes	reporter
T6015	31089-31094	NN	denotes	mouse
T6018	31095-31096	-LRB-	denotes	[
T6019	31096-31098	CD	denotes	20
T6020	31098-31099	-RRB-	denotes	]
T6008	31100-31104	VBD	denotes	gave
T6021	31105-31107	DT	denotes	no
T6022	31108-31113	NNS	denotes	signs
T6023	31114-31116	IN	denotes	of
T6024	31117-31124	JJ	denotes	ectopic
T6026	31125-31128	NN	denotes	LEF
T6027	31128-31129	HYPH	denotes	-
T6028	31129-31130	CD	denotes	1
T6029	31130-31131	HYPH	denotes	/
T6030	31131-31134	NN	denotes	Tcf
T6031	31134-31135	HYPH	denotes	/
T6032	31135-31136	NN	denotes	β
T6034	31136-31137	HYPH	denotes	-
T6033	31137-31144	NN	denotes	catenin
T6025	31145-31153	NN	denotes	activity
T6035	31154-31155	-LRB-	denotes	(
T6037	31155-31166	JJ	denotes	unpublished
T6036	31167-31171	NNS	denotes	data
T6038	31171-31172	-RRB-	denotes	)
T6039	31172-31173	.	denotes	.
T6040	31173-31314	sentence	denotes	We next turned to the presence of cytoplasmic Ajuba for a possible mechanistic link to the proliferative increase in our Snail Tg epidermis.
T6041	31174-31176	PRP	denotes	We
T6043	31177-31181	RB	denotes	next
T6042	31182-31188	VBD	denotes	turned
T6044	31189-31191	IN	denotes	to
T6045	31192-31195	DT	denotes	the
T6046	31196-31204	NN	denotes	presence
T6047	31205-31207	IN	denotes	of
T6048	31208-31219	JJ	denotes	cytoplasmic
T6049	31220-31225	NN	denotes	Ajuba
T6050	31226-31229	IN	denotes	for
T6051	31230-31231	DT	denotes	a
T6053	31232-31240	JJ	denotes	possible
T6054	31241-31252	JJ	denotes	mechanistic
T6052	31253-31257	NN	denotes	link
T6055	31258-31260	IN	denotes	to
T6056	31261-31264	DT	denotes	the
T6058	31265-31278	JJ	denotes	proliferative
T6057	31279-31287	NN	denotes	increase
T6059	31288-31290	IN	denotes	in
T6060	31291-31294	PRP$	denotes	our
T6062	31295-31300	NN	denotes	Snail
T6063	31301-31303	NN	denotes	Tg
T6061	31304-31313	NN	denotes	epidermis
T6064	31313-31314	.	denotes	.
T6065	31314-31564	sentence	denotes	In addition to its documented ability to bind α-catenin [10], Ajuba can also associate with growth factor receptor-bound protein-2 (Grb-2)/son of sevenless (Sos), the nucleotide exchange factor for Ras, which is upstream from activation of MAPK [9].
T6066	31315-31317	IN	denotes	In
T6068	31318-31326	NN	denotes	addition
T6069	31327-31329	IN	denotes	to
T6070	31330-31333	PRP$	denotes	its
T6072	31334-31344	VBN	denotes	documented
T6071	31345-31352	NN	denotes	ability
T6073	31353-31355	TO	denotes	to
T6074	31356-31360	VB	denotes	bind
T6075	31361-31362	NN	denotes	α
T6077	31362-31363	HYPH	denotes	-
T6076	31363-31370	NN	denotes	catenin
T6078	31371-31372	-LRB-	denotes	[
T6079	31372-31374	CD	denotes	10
T6080	31374-31375	-RRB-	denotes	]
T6081	31375-31377	,	denotes	,
T6082	31377-31382	NN	denotes	Ajuba
T6083	31383-31386	MD	denotes	can
T6084	31387-31391	RB	denotes	also
T6067	31392-31401	VB	denotes	associate
T6085	31402-31406	IN	denotes	with
T6086	31407-31413	NN	denotes	growth
T6087	31414-31420	NN	denotes	factor
T6088	31421-31429	NN	denotes	receptor
T6090	31429-31430	HYPH	denotes	-
T6089	31430-31435	JJ	denotes	bound
T6091	31436-31443	NN	denotes	protein
T6092	31443-31444	HYPH	denotes	-
T6093	31444-31445	CD	denotes	2
T6094	31446-31447	-LRB-	denotes	(
T6095	31447-31450	NN	denotes	Grb
T6096	31450-31451	HYPH	denotes	-
T6097	31451-31452	CD	denotes	2
T6098	31452-31453	-RRB-	denotes	)
T6099	31453-31454	HYPH	denotes	/
T6100	31454-31457	NN	denotes	son
T6101	31458-31460	IN	denotes	of
T6102	31461-31470	NN	denotes	sevenless
T6103	31471-31472	-LRB-	denotes	(
T6104	31472-31475	NN	denotes	Sos
T6105	31475-31476	-RRB-	denotes	)
T6106	31476-31478	,	denotes	,
T6107	31478-31481	DT	denotes	the
T6109	31482-31492	NN	denotes	nucleotide
T6110	31493-31501	NN	denotes	exchange
T6108	31502-31508	NN	denotes	factor
T6111	31509-31512	IN	denotes	for
T6112	31513-31516	NN	denotes	Ras
T6113	31516-31518	,	denotes	,
T6114	31518-31523	WDT	denotes	which
T6115	31524-31526	VBZ	denotes	is
T6116	31527-31535	RB	denotes	upstream
T6117	31536-31540	IN	denotes	from
T6118	31541-31551	NN	denotes	activation
T6119	31552-31554	IN	denotes	of
T6120	31555-31559	NN	denotes	MAPK
T6121	31560-31561	-LRB-	denotes	[
T6122	31561-31562	CD	denotes	9
T6123	31562-31563	-RRB-	denotes	]
T6124	31563-31564	.	denotes	.
T6125	31564-31722	sentence	denotes	Given the increase in pMAPK staining in Tg skin, we examined the possibility that Ajuba might have changed its binding partner in Snail-expressing epidermis.
T6126	31565-31570	VBN	denotes	Given
T6128	31571-31574	DT	denotes	the
T6129	31575-31583	NN	denotes	increase
T6130	31584-31586	IN	denotes	in
T6131	31587-31592	NN	denotes	pMAPK
T6132	31593-31601	NN	denotes	staining
T6133	31602-31604	IN	denotes	in
T6134	31605-31607	NN	denotes	Tg
T6135	31608-31612	NN	denotes	skin
T6136	31612-31614	,	denotes	,
T6137	31614-31616	PRP	denotes	we
T6127	31617-31625	VBD	denotes	examined
T6138	31626-31629	DT	denotes	the
T6139	31630-31641	NN	denotes	possibility
T6140	31642-31646	IN	denotes	that
T6142	31647-31652	NN	denotes	Ajuba
T6143	31653-31658	MD	denotes	might
T6144	31659-31663	VB	denotes	have
T6141	31664-31671	VBN	denotes	changed
T6145	31672-31675	PRP$	denotes	its
T6147	31676-31683	NN	denotes	binding
T6146	31684-31691	NN	denotes	partner
T6148	31692-31694	IN	denotes	in
T6149	31695-31700	NN	denotes	Snail
T6151	31700-31701	HYPH	denotes	-
T6150	31701-31711	VBG	denotes	expressing
T6152	31712-31721	NN	denotes	epidermis
T6153	31721-31722	.	denotes	.
T6154	31722-31911	sentence	denotes	Interestingly, Ajuba was readily detected in anti-Grb-2 immunoprecipitates of protein lysates from skins of Snail Tg mice but not from the corresponding wild-type (WT) animals (Figure 4E).
T6155	31723-31736	RB	denotes	Interestingly
T6157	31736-31738	,	denotes	,
T6158	31738-31743	NN	denotes	Ajuba
T6159	31744-31747	VBD	denotes	was
T6160	31748-31755	RB	denotes	readily
T6156	31756-31764	VBN	denotes	detected
T6161	31765-31767	IN	denotes	in
T6162	31768-31776	JJ	denotes	anti-Grb
T6164	31776-31777	HYPH	denotes	-
T6165	31777-31778	CD	denotes	2
T6163	31779-31797	NNS	denotes	immunoprecipitates
T6166	31798-31800	IN	denotes	of
T6167	31801-31808	NN	denotes	protein
T6168	31809-31816	NNS	denotes	lysates
T6169	31817-31821	IN	denotes	from
T6170	31822-31827	NNS	denotes	skins
T6171	31828-31830	IN	denotes	of
T6172	31831-31836	NN	denotes	Snail
T6174	31837-31839	NN	denotes	Tg
T6173	31840-31844	NNS	denotes	mice
T6175	31845-31848	CC	denotes	but
T6176	31849-31852	RB	denotes	not
T6177	31853-31857	IN	denotes	from
T6178	31858-31861	DT	denotes	the
T6180	31862-31875	JJ	denotes	corresponding
T6181	31876-31880	JJ	denotes	wild
T6183	31880-31881	HYPH	denotes	-
T6182	31881-31885	NN	denotes	type
T6184	31886-31887	-LRB-	denotes	(
T6185	31887-31889	NN	denotes	WT
T6186	31889-31890	-RRB-	denotes	)
T6179	31891-31898	NNS	denotes	animals
T6187	31899-31900	-LRB-	denotes	(
T6189	31900-31906	NN	denotes	Figure
T6188	31907-31909	NN	denotes	4E
T6190	31909-31910	-RRB-	denotes	)
T6191	31910-31911	.	denotes	.
T6192	31911-32138	sentence	denotes	When these experiments were repeated with α-catenin-null epidermis, a similar Grb-2-Ajuba association was detected, and again, this interaction was not detected in the protein extracts from control littermate skin (Figure 4E).
T6193	31912-31916	WRB	denotes	When
T6195	31917-31922	DT	denotes	these
T6196	31923-31934	NNS	denotes	experiments
T6197	31935-31939	VBD	denotes	were
T6194	31940-31948	VBN	denotes	repeated
T6199	31949-31953	IN	denotes	with
T6200	31954-31955	NN	denotes	α
T6202	31955-31956	HYPH	denotes	-
T6201	31956-31963	NN	denotes	catenin
T6204	31963-31964	HYPH	denotes	-
T6203	31964-31968	JJ	denotes	null
T6205	31969-31978	NN	denotes	epidermis
T6206	31978-31980	,	denotes	,
T6207	31980-31981	DT	denotes	a
T6209	31982-31989	JJ	denotes	similar
T6210	31990-31993	NN	denotes	Grb
T6212	31993-31994	HYPH	denotes	-
T6213	31994-31995	CD	denotes	2
T6214	31995-31996	HYPH	denotes	-
T6211	31996-32001	NN	denotes	Ajuba
T6208	32002-32013	NN	denotes	association
T6215	32014-32017	VBD	denotes	was
T6198	32018-32026	VBN	denotes	detected
T6216	32026-32028	,	denotes	,
T6217	32028-32031	CC	denotes	and
T6218	32032-32037	RB	denotes	again
T6220	32037-32039	,	denotes	,
T6221	32039-32043	DT	denotes	this
T6222	32044-32055	NN	denotes	interaction
T6223	32056-32059	VBD	denotes	was
T6224	32060-32063	RB	denotes	not
T6219	32064-32072	VBN	denotes	detected
T6225	32073-32075	IN	denotes	in
T6226	32076-32079	DT	denotes	the
T6228	32080-32087	NN	denotes	protein
T6227	32088-32096	NNS	denotes	extracts
T6229	32097-32101	IN	denotes	from
T6230	32102-32109	NN	denotes	control
T6232	32110-32120	NN	denotes	littermate
T6231	32121-32125	NN	denotes	skin
T6233	32126-32127	-LRB-	denotes	(
T6235	32127-32133	NN	denotes	Figure
T6234	32134-32136	NN	denotes	4E
T6236	32136-32137	-RRB-	denotes	)
T6237	32137-32138	.	denotes	.
T6238	32138-32346	sentence	denotes	Together, these data demonstrate that the reduction in α-catenin levels, either by Snail-mediated down-regulation of E-cadherin or by α-catenin conditional targeting, allows Ajuba to interact with Grb-2/Sos.
T6239	32139-32147	RB	denotes	Together
T6241	32147-32149	,	denotes	,
T6242	32149-32154	DT	denotes	these
T6243	32155-32159	NNS	denotes	data
T6240	32160-32171	VBP	denotes	demonstrate
T6244	32172-32176	IN	denotes	that
T6246	32177-32180	DT	denotes	the
T6247	32181-32190	NN	denotes	reduction
T6248	32191-32193	IN	denotes	in
T6249	32194-32195	NN	denotes	α
T6251	32195-32196	HYPH	denotes	-
T6250	32196-32203	NN	denotes	catenin
T6252	32204-32210	NNS	denotes	levels
T6253	32210-32212	,	denotes	,
T6254	32212-32218	CC	denotes	either
T6255	32219-32221	IN	denotes	by
T6256	32222-32227	NN	denotes	Snail
T6258	32227-32228	HYPH	denotes	-
T6257	32228-32236	VBN	denotes	mediated
T6260	32237-32241	JJ	denotes	down
T6261	32241-32242	HYPH	denotes	-
T6259	32242-32252	NN	denotes	regulation
T6262	32253-32255	IN	denotes	of
T6263	32256-32257	NN	denotes	E
T6265	32257-32258	HYPH	denotes	-
T6264	32258-32266	NN	denotes	cadherin
T6266	32267-32269	CC	denotes	or
T6267	32270-32272	IN	denotes	by
T6268	32273-32274	NN	denotes	α
T6270	32274-32275	HYPH	denotes	-
T6269	32275-32282	NN	denotes	catenin
T6271	32283-32294	JJ	denotes	conditional
T6272	32295-32304	NN	denotes	targeting
T6273	32304-32306	,	denotes	,
T6245	32306-32312	VBZ	denotes	allows
T6274	32313-32318	NN	denotes	Ajuba
T6276	32319-32321	TO	denotes	to
T6275	32322-32330	VB	denotes	interact
T6277	32331-32335	IN	denotes	with
T6278	32336-32339	NN	denotes	Grb
T6279	32339-32340	HYPH	denotes	-
T6280	32340-32341	CD	denotes	2
T6281	32341-32342	HYPH	denotes	/
T6282	32342-32345	NN	denotes	Sos
T6283	32345-32346	.	denotes	.
T6284	32346-32624	sentence	denotes	If the competition between Grb-2/Sos and α-catenin for Ajuba is functionally relevant to the hyperproliferative state of a keratinocyte, then overexpression of Ajuba would be expected to bypass the competition and promote activation of the Ras-MAPK pathway in WT keratinocytes.
T6285	32347-32349	IN	denotes	If
T6287	32350-32353	DT	denotes	the
T6288	32354-32365	NN	denotes	competition
T6289	32366-32373	IN	denotes	between
T6290	32374-32377	NN	denotes	Grb
T6291	32377-32378	HYPH	denotes	-
T6292	32378-32379	CD	denotes	2
T6293	32379-32380	HYPH	denotes	/
T6294	32380-32383	NN	denotes	Sos
T6295	32384-32387	CC	denotes	and
T6296	32388-32389	NN	denotes	α
T6298	32389-32390	HYPH	denotes	-
T6297	32390-32397	NN	denotes	catenin
T6299	32398-32401	IN	denotes	for
T6300	32402-32407	NN	denotes	Ajuba
T6286	32408-32410	VBZ	denotes	is
T6302	32411-32423	RB	denotes	functionally
T6303	32424-32432	JJ	denotes	relevant
T6304	32433-32435	IN	denotes	to
T6305	32436-32439	DT	denotes	the
T6307	32440-32458	JJ	denotes	hyperproliferative
T6306	32459-32464	NN	denotes	state
T6308	32465-32467	IN	denotes	of
T6309	32468-32469	DT	denotes	a
T6310	32470-32482	NN	denotes	keratinocyte
T6311	32482-32484	,	denotes	,
T6312	32484-32488	RB	denotes	then
T6313	32489-32503	NN	denotes	overexpression
T6314	32504-32506	IN	denotes	of
T6315	32507-32512	NN	denotes	Ajuba
T6316	32513-32518	MD	denotes	would
T6317	32519-32521	VB	denotes	be
T6301	32522-32530	VBN	denotes	expected
T6318	32531-32533	TO	denotes	to
T6319	32534-32540	VB	denotes	bypass
T6320	32541-32544	DT	denotes	the
T6321	32545-32556	NN	denotes	competition
T6322	32557-32560	CC	denotes	and
T6323	32561-32568	VB	denotes	promote
T6324	32569-32579	NN	denotes	activation
T6325	32580-32582	IN	denotes	of
T6326	32583-32586	DT	denotes	the
T6328	32587-32590	NN	denotes	Ras
T6330	32590-32591	HYPH	denotes	-
T6329	32591-32595	NN	denotes	MAPK
T6327	32596-32603	NN	denotes	pathway
T6331	32604-32606	IN	denotes	in
T6332	32607-32609	NN	denotes	WT
T6333	32610-32623	NNS	denotes	keratinocytes
T6334	32623-32624	.	denotes	.
T6335	32624-32855	sentence	denotes	Indeed, when serum-starved keratinocytes were transiently transfected with an Ajuba expression vector, the levels of pMAPK were not only elevated but also comparable to those transfected with the K14-HASnail transgene (Figure 4F).
T6336	32625-32631	RB	denotes	Indeed
T6338	32631-32633	,	denotes	,
T6339	32633-32637	WRB	denotes	when
T6341	32638-32643	NN	denotes	serum
T6343	32643-32644	HYPH	denotes	-
T6342	32644-32651	VBN	denotes	starved
T6344	32652-32665	NNS	denotes	keratinocytes
T6345	32666-32670	VBD	denotes	were
T6346	32671-32682	RB	denotes	transiently
T6340	32683-32694	VBN	denotes	transfected
T6347	32695-32699	IN	denotes	with
T6348	32700-32702	DT	denotes	an
T6350	32703-32708	NN	denotes	Ajuba
T6351	32709-32719	NN	denotes	expression
T6349	32720-32726	NN	denotes	vector
T6352	32726-32728	,	denotes	,
T6353	32728-32731	DT	denotes	the
T6354	32732-32738	NNS	denotes	levels
T6355	32739-32741	IN	denotes	of
T6356	32742-32747	NN	denotes	pMAPK
T6337	32748-32752	VBD	denotes	were
T6357	32753-32756	RB	denotes	not
T6359	32757-32761	RB	denotes	only
T6358	32762-32770	JJ	denotes	elevated
T6360	32771-32774	CC	denotes	but
T6361	32775-32779	RB	denotes	also
T6362	32780-32790	JJ	denotes	comparable
T6363	32791-32793	IN	denotes	to
T6364	32794-32799	DT	denotes	those
T6365	32800-32811	VBN	denotes	transfected
T6366	32812-32816	IN	denotes	with
T6367	32817-32820	DT	denotes	the
T6369	32821-32824	NN	denotes	K14
T6371	32824-32825	HYPH	denotes	-
T6370	32825-32832	NN	denotes	HASnail
T6368	32833-32842	NN	denotes	transgene
T6372	32843-32844	-LRB-	denotes	(
T6374	32844-32850	NN	denotes	Figure
T6373	32851-32853	NN	denotes	4F
T6375	32853-32854	-RRB-	denotes	)
T6376	32854-32855	.	denotes	.
T6377	32855-33037	sentence	denotes	This activation was abolished when cells were treated with a small peptide inhibitor that specifically interrupts the Grb-2/Sos interaction (Figure 4F; see lanes marked “inh”) [27].
T6378	32856-32860	DT	denotes	This
T6379	32861-32871	NN	denotes	activation
T6381	32872-32875	VBD	denotes	was
T6380	32876-32885	VBN	denotes	abolished
T6382	32886-32890	WRB	denotes	when
T6384	32891-32896	NNS	denotes	cells
T6385	32897-32901	VBD	denotes	were
T6383	32902-32909	VBN	denotes	treated
T6386	32910-32914	IN	denotes	with
T6387	32915-32916	DT	denotes	a
T6389	32917-32922	JJ	denotes	small
T6390	32923-32930	NN	denotes	peptide
T6388	32931-32940	NN	denotes	inhibitor
T6391	32941-32945	WDT	denotes	that
T6393	32946-32958	RB	denotes	specifically
T6392	32959-32969	VBZ	denotes	interrupts
T6394	32970-32973	DT	denotes	the
T6396	32974-32977	NN	denotes	Grb
T6397	32977-32978	HYPH	denotes	-
T6398	32978-32979	CD	denotes	2
T6399	32979-32980	HYPH	denotes	/
T6400	32980-32983	NN	denotes	Sos
T6395	32984-32995	NN	denotes	interaction
T6401	32996-32997	-LRB-	denotes	(
T6403	32997-33003	NN	denotes	Figure
T6402	33004-33006	NN	denotes	4F
T6404	33006-33007	:	denotes	;
T6405	33008-33011	VB	denotes	see
T6406	33012-33017	NNS	denotes	lanes
T6407	33018-33024	VBN	denotes	marked
T6408	33025-33026	``	denotes	“
T6409	33026-33029	NN	denotes	inh
T6410	33029-33030	''	denotes	”
T6411	33030-33031	-RRB-	denotes	)
T6412	33032-33033	-LRB-	denotes	[
T6413	33033-33035	CD	denotes	27
T6414	33035-33036	-RRB-	denotes	]
T6415	33036-33037	.	denotes	.
T6416	33037-33131	sentence	denotes	Ajuba's pre-LIM domain is the segment that associates with Grb-2's Src-homology 3 domain [9].
T6417	33038-33043	NN	denotes	Ajuba
T6419	33043-33045	POS	denotes	's
T6420	33046-33053	JJ	denotes	pre-LIM
T6418	33054-33060	NN	denotes	domain
T6421	33061-33063	VBZ	denotes	is
T6422	33064-33067	DT	denotes	the
T6423	33068-33075	NN	denotes	segment
T6424	33076-33080	WDT	denotes	that
T6425	33081-33091	VBZ	denotes	associates
T6426	33092-33096	IN	denotes	with
T6427	33097-33100	NN	denotes	Grb
T6429	33100-33101	HYPH	denotes	-
T6430	33101-33102	CD	denotes	2
T6431	33102-33104	POS	denotes	's
T6432	33105-33108	NN	denotes	Src
T6434	33108-33109	HYPH	denotes	-
T6433	33109-33117	NN	denotes	homology
T6435	33118-33119	CD	denotes	3
T6428	33120-33126	NN	denotes	domain
T6436	33127-33128	-LRB-	denotes	[
T6437	33128-33129	CD	denotes	9
T6438	33129-33130	-RRB-	denotes	]
T6439	33130-33131	.	denotes	.
T6440	33131-33256	sentence	denotes	When this domain was overexpressed in serum-starved keratinocytes, a comparable elevation in pMAPK was observed (Figure 4F).
T6441	33132-33136	WRB	denotes	When
T6443	33137-33141	DT	denotes	this
T6444	33142-33148	NN	denotes	domain
T6445	33149-33152	VBD	denotes	was
T6442	33153-33166	VBN	denotes	overexpressed
T6447	33167-33169	IN	denotes	in
T6448	33170-33175	NN	denotes	serum
T6450	33175-33176	HYPH	denotes	-
T6449	33176-33183	VBN	denotes	starved
T6451	33184-33197	NNS	denotes	keratinocytes
T6452	33197-33199	,	denotes	,
T6453	33199-33200	DT	denotes	a
T6455	33201-33211	JJ	denotes	comparable
T6454	33212-33221	NN	denotes	elevation
T6456	33222-33224	IN	denotes	in
T6457	33225-33230	NN	denotes	pMAPK
T6458	33231-33234	VBD	denotes	was
T6446	33235-33243	VBN	denotes	observed
T6459	33244-33245	-LRB-	denotes	(
T6461	33245-33251	NN	denotes	Figure
T6460	33252-33254	NN	denotes	4F
T6462	33254-33255	-RRB-	denotes	)
T6463	33255-33256	.	denotes	.
T6464	33256-33360	sentence	denotes	As expected, the small peptide inhibitor that interrupts the Grb-2/Sos association blocked the effects.
T6465	33257-33259	IN	denotes	As
T6466	33260-33268	VBN	denotes	expected
T6468	33268-33270	,	denotes	,
T6469	33270-33273	DT	denotes	the
T6471	33274-33279	JJ	denotes	small
T6472	33280-33287	NN	denotes	peptide
T6470	33288-33297	NN	denotes	inhibitor
T6473	33298-33302	WDT	denotes	that
T6474	33303-33313	VBZ	denotes	interrupts
T6475	33314-33317	DT	denotes	the
T6477	33318-33321	NN	denotes	Grb
T6478	33321-33322	HYPH	denotes	-
T6479	33322-33323	CD	denotes	2
T6480	33323-33324	HYPH	denotes	/
T6481	33324-33327	NN	denotes	Sos
T6476	33328-33339	NN	denotes	association
T6467	33340-33347	VBD	denotes	blocked
T6482	33348-33351	DT	denotes	the
T6483	33352-33359	NNS	denotes	effects
T6484	33359-33360	.	denotes	.
T6485	33360-33585	sentence	denotes	These data suggested that by elevating cytosolic Ajuba levels, Ajuba's pre-LIM domain may associate with Grb-2/Sos in a manner that stimulates its nucleotide exchange activity and leads to activation of the Ras-MAPK pathway.
T6486	33361-33366	DT	denotes	These
T6487	33367-33371	NNS	denotes	data
T6488	33372-33381	VBD	denotes	suggested
T6489	33382-33386	IN	denotes	that
T6491	33387-33389	IN	denotes	by
T6492	33390-33399	VBG	denotes	elevating
T6493	33400-33409	JJ	denotes	cytosolic
T6495	33410-33415	NN	denotes	Ajuba
T6494	33416-33422	NNS	denotes	levels
T6496	33422-33424	,	denotes	,
T6497	33424-33429	NN	denotes	Ajuba
T6499	33429-33431	POS	denotes	's
T6500	33432-33439	JJ	denotes	pre-LIM
T6498	33440-33446	NN	denotes	domain
T6501	33447-33450	MD	denotes	may
T6490	33451-33460	VB	denotes	associate
T6502	33461-33465	IN	denotes	with
T6503	33466-33469	NN	denotes	Grb
T6504	33469-33470	HYPH	denotes	-
T6505	33470-33471	CD	denotes	2
T6506	33471-33472	HYPH	denotes	/
T6507	33472-33475	NN	denotes	Sos
T6508	33476-33478	IN	denotes	in
T6509	33479-33480	DT	denotes	a
T6510	33481-33487	NN	denotes	manner
T6511	33488-33492	WDT	denotes	that
T6512	33493-33503	VBZ	denotes	stimulates
T6513	33504-33507	PRP$	denotes	its
T6515	33508-33518	NN	denotes	nucleotide
T6516	33519-33527	NN	denotes	exchange
T6514	33528-33536	NN	denotes	activity
T6517	33537-33540	CC	denotes	and
T6518	33541-33546	VBZ	denotes	leads
T6519	33547-33549	IN	denotes	to
T6520	33550-33560	NN	denotes	activation
T6521	33561-33563	IN	denotes	of
T6522	33564-33567	DT	denotes	the
T6524	33568-33571	NN	denotes	Ras
T6526	33571-33572	HYPH	denotes	-
T6525	33572-33576	NN	denotes	MAPK
T6523	33577-33584	NN	denotes	pathway
T6527	33584-33585	.	denotes	.
T6528	33585-33795	sentence	denotes	Although this pathway provides one mechanism by which Snail expression and proliferation may be coupled in skin epithelium, proliferative circuitries involving AJs are known to be complex and often interwoven.
T6529	33586-33594	IN	denotes	Although
T6531	33595-33599	DT	denotes	this
T6532	33600-33607	NN	denotes	pathway
T6530	33608-33616	VBZ	denotes	provides
T6534	33617-33620	CD	denotes	one
T6535	33621-33630	NN	denotes	mechanism
T6536	33631-33633	IN	denotes	by
T6538	33634-33639	WDT	denotes	which
T6539	33640-33645	NN	denotes	Snail
T6540	33646-33656	NN	denotes	expression
T6541	33657-33660	CC	denotes	and
T6542	33661-33674	NN	denotes	proliferation
T6543	33675-33678	MD	denotes	may
T6544	33679-33681	VB	denotes	be
T6537	33682-33689	VBN	denotes	coupled
T6545	33690-33692	IN	denotes	in
T6546	33693-33697	NN	denotes	skin
T6547	33698-33708	NN	denotes	epithelium
T6548	33708-33710	,	denotes	,
T6549	33710-33723	JJ	denotes	proliferative
T6550	33724-33735	NNS	denotes	circuitries
T6551	33736-33745	VBG	denotes	involving
T6552	33746-33749	NNS	denotes	AJs
T6553	33750-33753	VBP	denotes	are
T6533	33754-33759	VBN	denotes	known
T6554	33760-33762	TO	denotes	to
T6555	33763-33765	VB	denotes	be
T6556	33766-33773	JJ	denotes	complex
T6557	33774-33777	CC	denotes	and
T6558	33778-33783	RB	denotes	often
T6559	33784-33794	VBN	denotes	interwoven
T6560	33794-33795	.	denotes	.
T6561	33795-33900	sentence	denotes	Future studies will be needed to systematically dissect these putative intricacies at a molecular level.
T6562	33796-33802	JJ	denotes	Future
T6563	33803-33810	NNS	denotes	studies
T6565	33811-33815	MD	denotes	will
T6566	33816-33818	VB	denotes	be
T6564	33819-33825	VBN	denotes	needed
T6567	33826-33828	TO	denotes	to
T6569	33829-33843	RB	denotes	systematically
T6568	33844-33851	VB	denotes	dissect
T6570	33852-33857	DT	denotes	these
T6572	33858-33866	JJ	denotes	putative
T6571	33867-33878	NNS	denotes	intricacies
T6573	33879-33881	IN	denotes	at
T6574	33882-33883	DT	denotes	a
T6576	33884-33893	JJ	denotes	molecular
T6575	33894-33899	NN	denotes	level
T6577	33899-33900	.	denotes	.
T7354	33902-33909	VBG	denotes	Probing
T7356	33910-33913	DT	denotes	the
T7357	33914-33924	NN	denotes	Regulation
T7358	33925-33927	IN	denotes	of
T7359	33928-33933	NN	denotes	Snail
T7360	33934-33938	NN	denotes	Gene
T7361	33939-33949	NN	denotes	Expression
T7355	33950-33957	VBZ	denotes	Reveals
T7362	33958-33960	DT	denotes	an
T7364	33961-33970	JJ	denotes	Essential
T7363	33971-33975	NN	denotes	Link
T7365	33976-33978	IN	denotes	to
T7366	33979-33982	NN	denotes	TGF
T7368	33982-33983	HYPH	denotes	-
T7367	33983-33985	NN	denotes	β2
T7369	33986-33995	NN	denotes	Signaling
T7370	33996-33998	IN	denotes	in
T7371	33999-34002	DT	denotes	the
T7373	34003-34013	VBG	denotes	Developing
T7374	34014-34018	NN	denotes	Hair
T7372	34019-34022	NN	denotes	Bud
T7375	34022-34155	sentence	denotes	The temporal spike of Snail mRNA expression in the hair bud prompted us to consider what factor(s) may be regulating the Snail gene.
T7376	34023-34026	DT	denotes	The
T7378	34027-34035	JJ	denotes	temporal
T7377	34036-34041	NN	denotes	spike
T7380	34042-34044	IN	denotes	of
T7381	34045-34050	NN	denotes	Snail
T7383	34051-34055	NN	denotes	mRNA
T7382	34056-34066	NN	denotes	expression
T7384	34067-34069	IN	denotes	in
T7385	34070-34073	DT	denotes	the
T7387	34074-34078	NN	denotes	hair
T7386	34079-34082	NN	denotes	bud
T7379	34083-34091	VBD	denotes	prompted
T7388	34092-34094	PRP	denotes	us
T7389	34095-34097	TO	denotes	to
T7390	34098-34106	VB	denotes	consider
T7391	34107-34111	WDT	denotes	what
T7392	34112-34118	NN	denotes	factor
T7394	34118-34119	-LRB-	denotes	(
T7395	34119-34120	AFX	denotes	s
T7396	34120-34121	-RRB-	denotes	)
T7397	34122-34125	MD	denotes	may
T7398	34126-34128	VB	denotes	be
T7393	34129-34139	VBG	denotes	regulating
T7399	34140-34143	DT	denotes	the
T7401	34144-34149	NN	denotes	Snail
T7400	34150-34154	NN	denotes	gene
T7402	34154-34155	.	denotes	.
T7403	34155-34379	sentence	denotes	A variety of extracellular signals have an impact on the cell type-specific expression of different Snail family members, and many of them, including Wnts, BMPs, FGFs, and TGF-βs, also affect hair bud development [2,16,28].
T7404	34156-34157	DT	denotes	A
T7405	34158-34165	NN	denotes	variety
T7407	34166-34168	IN	denotes	of
T7408	34169-34182	JJ	denotes	extracellular
T7409	34183-34190	NNS	denotes	signals
T7406	34191-34195	VBP	denotes	have
T7410	34196-34198	DT	denotes	an
T7411	34199-34205	NN	denotes	impact
T7412	34206-34208	IN	denotes	on
T7413	34209-34212	DT	denotes	the
T7415	34213-34217	NN	denotes	cell
T7416	34218-34222	NN	denotes	type
T7418	34222-34223	HYPH	denotes	-
T7417	34223-34231	JJ	denotes	specific
T7414	34232-34242	NN	denotes	expression
T7419	34243-34245	IN	denotes	of
T7420	34246-34255	JJ	denotes	different
T7422	34256-34261	NN	denotes	Snail
T7423	34262-34268	NN	denotes	family
T7421	34269-34276	NNS	denotes	members
T7424	34276-34278	,	denotes	,
T7425	34278-34281	CC	denotes	and
T7426	34282-34286	JJ	denotes	many
T7428	34287-34289	IN	denotes	of
T7429	34290-34294	PRP	denotes	them
T7430	34294-34296	,	denotes	,
T7431	34296-34305	VBG	denotes	including
T7432	34306-34310	NNS	denotes	Wnts
T7433	34310-34312	,	denotes	,
T7434	34312-34316	NNS	denotes	BMPs
T7435	34316-34318	,	denotes	,
T7436	34318-34322	NNS	denotes	FGFs
T7437	34322-34324	,	denotes	,
T7438	34324-34327	CC	denotes	and
T7439	34328-34331	NN	denotes	TGF
T7441	34331-34332	HYPH	denotes	-
T7440	34332-34334	NNS	denotes	βs
T7442	34334-34336	,	denotes	,
T7443	34336-34340	RB	denotes	also
T7427	34341-34347	VBP	denotes	affect
T7444	34348-34352	NN	denotes	hair
T7445	34353-34356	NN	denotes	bud
T7446	34357-34368	NN	denotes	development
T7447	34369-34370	-LRB-	denotes	[
T7449	34370-34371	CD	denotes	2
T7450	34371-34372	,	denotes	,
T7451	34372-34374	CD	denotes	16
T7452	34374-34375	,	denotes	,
T7448	34375-34377	CD	denotes	28
T7453	34377-34378	-RRB-	denotes	]
T7454	34378-34379	.	denotes	.
T7455	34379-34607	sentence	denotes	Since Snail is not expressed in cultured skin keratinocytes that secrete active BMPs and FGFs (see Figure 1B), we focused our attention on Wnt and TGF-β signaling as more likely candidates for Snail induction in this cell type.
T7456	34380-34385	IN	denotes	Since
T7458	34386-34391	NN	denotes	Snail
T7459	34392-34394	VBZ	denotes	is
T7460	34395-34398	RB	denotes	not
T7457	34399-34408	VBN	denotes	expressed
T7462	34409-34411	IN	denotes	in
T7463	34412-34420	VBN	denotes	cultured
T7465	34421-34425	NN	denotes	skin
T7464	34426-34439	NNS	denotes	keratinocytes
T7466	34440-34444	WDT	denotes	that
T7467	34445-34452	VBP	denotes	secrete
T7468	34453-34459	JJ	denotes	active
T7469	34460-34464	NNS	denotes	BMPs
T7470	34465-34468	CC	denotes	and
T7471	34469-34473	NNS	denotes	FGFs
T7472	34474-34475	-LRB-	denotes	(
T7473	34475-34478	VB	denotes	see
T7474	34479-34485	NN	denotes	Figure
T7475	34486-34488	NN	denotes	1B
T7476	34488-34489	-RRB-	denotes	)
T7477	34489-34491	,	denotes	,
T7478	34491-34493	PRP	denotes	we
T7461	34494-34501	VBD	denotes	focused
T7479	34502-34505	PRP$	denotes	our
T7480	34506-34515	NN	denotes	attention
T7481	34516-34518	IN	denotes	on
T7482	34519-34522	NN	denotes	Wnt
T7484	34523-34526	CC	denotes	and
T7485	34527-34530	NN	denotes	TGF
T7487	34530-34531	HYPH	denotes	-
T7486	34531-34532	NN	denotes	β
T7483	34533-34542	NN	denotes	signaling
T7488	34543-34545	IN	denotes	as
T7489	34546-34550	RBR	denotes	more
T7490	34551-34557	JJ	denotes	likely
T7491	34558-34568	NNS	denotes	candidates
T7492	34569-34572	IN	denotes	for
T7493	34573-34578	NN	denotes	Snail
T7494	34579-34588	NN	denotes	induction
T7495	34589-34591	IN	denotes	in
T7496	34592-34596	DT	denotes	this
T7498	34597-34601	NN	denotes	cell
T7497	34602-34606	NN	denotes	type
T7499	34606-34607	.	denotes	.
T7500	34607-34806	sentence	denotes	Previously, we showed that effective transmission of a Wnt-3a signal in cultured keratinocytes can be achieved through their exposure to the BMP inhibitor noggin, which induces LEF-1 expression [4].
T7501	34608-34618	RB	denotes	Previously
T7503	34618-34620	,	denotes	,
T7504	34620-34622	PRP	denotes	we
T7502	34623-34629	VBD	denotes	showed
T7505	34630-34634	IN	denotes	that
T7507	34635-34644	JJ	denotes	effective
T7508	34645-34657	NN	denotes	transmission
T7509	34658-34660	IN	denotes	of
T7510	34661-34662	DT	denotes	a
T7512	34663-34666	NN	denotes	Wnt
T7513	34666-34667	HYPH	denotes	-
T7514	34667-34669	CD	denotes	3a
T7511	34670-34676	NN	denotes	signal
T7515	34677-34679	IN	denotes	in
T7516	34680-34688	VBN	denotes	cultured
T7517	34689-34702	NNS	denotes	keratinocytes
T7518	34703-34706	MD	denotes	can
T7519	34707-34709	VB	denotes	be
T7506	34710-34718	VBN	denotes	achieved
T7520	34719-34726	IN	denotes	through
T7521	34727-34732	PRP$	denotes	their
T7522	34733-34741	NN	denotes	exposure
T7523	34742-34744	IN	denotes	to
T7524	34745-34748	DT	denotes	the
T7526	34749-34752	NN	denotes	BMP
T7525	34753-34762	NN	denotes	inhibitor
T7527	34763-34769	NN	denotes	noggin
T7528	34769-34771	,	denotes	,
T7529	34771-34776	WDT	denotes	which
T7530	34777-34784	VBZ	denotes	induces
T7531	34785-34788	NN	denotes	LEF
T7533	34788-34789	HYPH	denotes	-
T7534	34789-34790	CD	denotes	1
T7532	34791-34801	NN	denotes	expression
T7535	34802-34803	-LRB-	denotes	[
T7536	34803-34804	CD	denotes	4
T7537	34804-34805	-RRB-	denotes	]
T7538	34805-34806	.	denotes	.
T7539	34806-34941	sentence	denotes	In vitro, these conditions down-regulated the E-cadherin promoter and induced a LEF-1/β-catenin-sensitive reporter gene, TOPFLASH [4].
T7540	34807-34809	FW	denotes	In
T7541	34810-34815	FW	denotes	vitro
T7543	34815-34817	,	denotes	,
T7544	34817-34822	DT	denotes	these
T7545	34823-34833	NNS	denotes	conditions
T7546	34834-34838	RB	denotes	down
T7547	34838-34839	HYPH	denotes	-
T7542	34839-34848	VBD	denotes	regulated
T7548	34849-34852	DT	denotes	the
T7550	34853-34854	NN	denotes	E
T7552	34854-34855	HYPH	denotes	-
T7551	34855-34863	NN	denotes	cadherin
T7549	34864-34872	NN	denotes	promoter
T7553	34873-34876	CC	denotes	and
T7554	34877-34884	VBD	denotes	induced
T7555	34885-34886	DT	denotes	a
T7557	34887-34890	NN	denotes	LEF
T7559	34890-34891	HYPH	denotes	-
T7560	34891-34892	CD	denotes	1
T7561	34892-34893	HYPH	denotes	/
T7562	34893-34894	NN	denotes	β
T7564	34894-34895	HYPH	denotes	-
T7563	34895-34902	NN	denotes	catenin
T7565	34902-34903	HYPH	denotes	-
T7558	34903-34912	JJ	denotes	sensitive
T7566	34913-34921	NN	denotes	reporter
T7556	34922-34926	NN	denotes	gene
T7567	34926-34928	,	denotes	,
T7568	34928-34936	NN	denotes	TOPFLASH
T7569	34937-34938	-LRB-	denotes	[
T7570	34938-34939	CD	denotes	4
T7571	34939-34940	-RRB-	denotes	]
T7572	34940-34941	.	denotes	.
T7573	34941-35020	sentence	denotes	In contrast, Snail expression was not induced by these conditions (Figure 5A).
T7574	34942-34944	IN	denotes	In
T7576	34945-34953	NN	denotes	contrast
T7577	34953-34955	,	denotes	,
T7578	34955-34960	NN	denotes	Snail
T7579	34961-34971	NN	denotes	expression
T7580	34972-34975	VBD	denotes	was
T7581	34976-34979	RB	denotes	not
T7575	34980-34987	VBN	denotes	induced
T7582	34988-34990	IN	denotes	by
T7583	34991-34996	DT	denotes	these
T7584	34997-35007	NNS	denotes	conditions
T7585	35008-35009	-LRB-	denotes	(
T7587	35009-35015	NN	denotes	Figure
T7586	35016-35018	NN	denotes	5A
T7588	35018-35019	-RRB-	denotes	)
T7589	35019-35020	.	denotes	.
T7590	35020-35224	sentence	denotes	Thus, despite essential roles for Wnts and noggin in hair follicle specification [4,29,30], our studies did not support an essential role for these signals in governing Snail expression in keratinocytes.
T7591	35021-35025	RB	denotes	Thus
T7593	35025-35027	,	denotes	,
T7594	35027-35034	IN	denotes	despite
T7595	35035-35044	JJ	denotes	essential
T7596	35045-35050	NNS	denotes	roles
T7597	35051-35054	IN	denotes	for
T7598	35055-35059	NNS	denotes	Wnts
T7599	35060-35063	CC	denotes	and
T7600	35064-35070	NN	denotes	noggin
T7601	35071-35073	IN	denotes	in
T7602	35074-35078	NN	denotes	hair
T7603	35079-35087	NN	denotes	follicle
T7604	35088-35101	NN	denotes	specification
T7605	35102-35103	-LRB-	denotes	[
T7607	35103-35104	CD	denotes	4
T7608	35104-35105	,	denotes	,
T7609	35105-35107	CD	denotes	29
T7610	35107-35108	,	denotes	,
T7606	35108-35110	CD	denotes	30
T7611	35110-35111	-RRB-	denotes	]
T7612	35111-35113	,	denotes	,
T7613	35113-35116	PRP$	denotes	our
T7614	35117-35124	NNS	denotes	studies
T7615	35125-35128	VBD	denotes	did
T7616	35129-35132	RB	denotes	not
T7592	35133-35140	VB	denotes	support
T7617	35141-35143	DT	denotes	an
T7619	35144-35153	JJ	denotes	essential
T7618	35154-35158	NN	denotes	role
T7620	35159-35162	IN	denotes	for
T7621	35163-35168	DT	denotes	these
T7622	35169-35176	NNS	denotes	signals
T7623	35177-35179	IN	denotes	in
T7624	35180-35189	VBG	denotes	governing
T7625	35190-35195	NN	denotes	Snail
T7626	35196-35206	NN	denotes	expression
T7627	35207-35209	IN	denotes	in
T7628	35210-35223	NNS	denotes	keratinocytes
T7629	35223-35224	.	denotes	.
T7630	35224-37423	sentence	denotes	Figure 5 TGF-β2, but Not Wnt/noggin, Transiently Induces Snail Expression In Vitro (A) Failure of Wnt and noggin signaling to induce Snail in cultured keratinocytes. Primary mouse keratinocytes were treated with Wnt- and/or noggin-conditioned medium (+) or the corresponding control medium (–). These conditions are known to activate the LEF-1/β-catenin reporter TOPGal and down-regulate the E-cadherin promoter (see [4] for details). Using Western blot analyses, cellular proteins were then analyzed for Snail, LEF-1, β-catenin, and tubulin. Proteins from keratinocytes transfected with K14-Snail were used as a positive control for Snail expression. (B) TGF-β2 can induce Snail protein. Primary keratinocytes were treated for the indicated times with recombinant TGF-β2 (+) or heat inactivated TGF-β2 (–).Total cellular proteins were then isolated and analyzed by Western blot for Snail, pSMAD2 (reflective of activated TGF- signaling), and tubulin. Note the activation of Snail expression, peaking at 2 h post-TGF-β2 treatment and then disappearing thereafter. (C) Snail mRNA expression is transiently induced by TGF-β2. The experiment in (B) was repeated, and this time, total RNAs were isolated from keratinocytes treated with TGF-β2 for the indicated times. RT-PCR was then used with (+) or without (–) reverse transcriptase (RT) and with primer sets specific for Snail and GAPDH mRNAs. Note that Snail mRNA expression also peaked at 2 h, paralleling Snail protein. (D) TGF-β2 treatment results in enhanced activity of a Snail promoter-β-galactosidase reporter. Keratinocytes were transfected with a β-galactosidase reporter driven by a Snail promoter that is either WT (wt prom) or harbors a mutation in a putative pSMAD2/pSMAD4 binding site (mt prom). At 2 d posttransfection, cells were treated with either TGF-β or heat-inactivated TGF-β2 (inact) for the times indicated. β-galactosidase assays were then conducted, and results are reported as fold increase over a basal level of activity of 1. The experiment was repeated three times in triplicate, and error bars reflect variations in the results. TGF-β1 has been shown to induce Snail family members in hepatocytes and heart [15, 31].
T7631	37336-37339	NN	denotes	TGF
T7633	37339-37340	HYPH	denotes	-
T7632	37340-37342	NN	denotes	β1
T7635	37343-37346	VBZ	denotes	has
T7636	37347-37351	VBN	denotes	been
T7634	37352-37357	VBN	denotes	shown
T7637	37358-37360	TO	denotes	to
T7638	37361-37367	VB	denotes	induce
T7639	37368-37373	NN	denotes	Snail
T7641	37374-37380	NN	denotes	family
T7640	37381-37388	NNS	denotes	members
T7642	37389-37391	IN	denotes	in
T7643	37392-37403	NNS	denotes	hepatocytes
T7644	37404-37407	CC	denotes	and
T7645	37408-37413	NN	denotes	heart
T7646	37414-37415	-LRB-	denotes	[
T7648	37415-37417	CD	denotes	15
T7649	37417-37419	,	denotes	,
T7647	37419-37421	CD	denotes	31
T7650	37421-37422	-RRB-	denotes	]
T7651	37422-37423	.	denotes	.
T7652	37423-37582	sentence	denotes	In keratinocytes, however, TGF-β1 inhibits keratinocyte growth and seems to be involved in triggering the destructive phase of the cycling hair follicle [32].
T7653	37424-37426	IN	denotes	In
T7655	37427-37440	NNS	denotes	keratinocytes
T7656	37440-37442	,	denotes	,
T7657	37442-37449	RB	denotes	however
T7658	37449-37451	,	denotes	,
T7659	37451-37454	NN	denotes	TGF
T7661	37454-37455	HYPH	denotes	-
T7660	37455-37457	NN	denotes	β1
T7654	37458-37466	VBZ	denotes	inhibits
T7662	37467-37479	NN	denotes	keratinocyte
T7663	37480-37486	NN	denotes	growth
T7664	37487-37490	CC	denotes	and
T7665	37491-37496	VBZ	denotes	seems
T7666	37497-37499	TO	denotes	to
T7668	37500-37502	VB	denotes	be
T7667	37503-37511	VBN	denotes	involved
T7669	37512-37514	IN	denotes	in
T7670	37515-37525	VBG	denotes	triggering
T7671	37526-37529	DT	denotes	the
T7673	37530-37541	JJ	denotes	destructive
T7672	37542-37547	NN	denotes	phase
T7674	37548-37550	IN	denotes	of
T7675	37551-37554	DT	denotes	the
T7677	37555-37562	VBG	denotes	cycling
T7678	37563-37567	NN	denotes	hair
T7676	37568-37576	NN	denotes	follicle
T7679	37577-37578	-LRB-	denotes	[
T7680	37578-37580	CD	denotes	32
T7681	37580-37581	-RRB-	denotes	]
T7682	37581-37582	.	denotes	.
T7683	37582-37738	sentence	denotes	Of the loss-of-function mutations generated in each of the TGF-β genes, only the TGF-β2 null state blocked follicle development at the hair bud stage [32].
T7684	37583-37585	IN	denotes	Of
T7686	37586-37589	DT	denotes	the
T7688	37590-37594	NN	denotes	loss
T7689	37594-37595	HYPH	denotes	-
T7690	37595-37597	IN	denotes	of
T7691	37597-37598	HYPH	denotes	-
T7692	37598-37606	NN	denotes	function
T7687	37607-37616	NNS	denotes	mutations
T7693	37617-37626	VBN	denotes	generated
T7694	37627-37629	IN	denotes	in
T7695	37630-37634	DT	denotes	each
T7696	37635-37637	IN	denotes	of
T7697	37638-37641	DT	denotes	the
T7699	37642-37645	NN	denotes	TGF
T7701	37645-37646	HYPH	denotes	-
T7700	37646-37647	NN	denotes	β
T7698	37648-37653	NNS	denotes	genes
T7702	37653-37655	,	denotes	,
T7703	37655-37659	RB	denotes	only
T7705	37660-37663	DT	denotes	the
T7706	37664-37667	NN	denotes	TGF
T7708	37667-37668	HYPH	denotes	-
T7707	37668-37670	NN	denotes	β2
T7709	37671-37675	JJ	denotes	null
T7704	37676-37681	NN	denotes	state
T7685	37682-37689	VBD	denotes	blocked
T7710	37690-37698	NN	denotes	follicle
T7711	37699-37710	NN	denotes	development
T7712	37711-37713	IN	denotes	at
T7713	37714-37717	DT	denotes	the
T7715	37718-37722	NN	denotes	hair
T7716	37723-37726	NN	denotes	bud
T7714	37727-37732	NN	denotes	stage
T7717	37733-37734	-LRB-	denotes	[
T7718	37734-37736	CD	denotes	32
T7719	37736-37737	-RRB-	denotes	]
T7720	37737-37738	.	denotes	.
T7721	37738-37902	sentence	denotes	Thus, we turned towards addressing whether TGF-β2 might be involved in regulating Snail expression in keratinocytes isolated from the basal layer of the epidermis.
T7722	37739-37743	RB	denotes	Thus
T7724	37743-37745	,	denotes	,
T7725	37745-37747	PRP	denotes	we
T7723	37748-37754	VBD	denotes	turned
T7726	37755-37762	IN	denotes	towards
T7727	37763-37773	VBG	denotes	addressing
T7728	37774-37781	IN	denotes	whether
T7730	37782-37785	NN	denotes	TGF
T7732	37785-37786	HYPH	denotes	-
T7731	37786-37788	NN	denotes	β2
T7733	37789-37794	MD	denotes	might
T7734	37795-37797	VB	denotes	be
T7729	37798-37806	VBN	denotes	involved
T7735	37807-37809	IN	denotes	in
T7736	37810-37820	VBG	denotes	regulating
T7737	37821-37826	NN	denotes	Snail
T7738	37827-37837	NN	denotes	expression
T7739	37838-37840	IN	denotes	in
T7740	37841-37854	NNS	denotes	keratinocytes
T7741	37855-37863	VBN	denotes	isolated
T7742	37864-37868	IN	denotes	from
T7743	37869-37872	DT	denotes	the
T7745	37873-37878	JJ	denotes	basal
T7744	37879-37884	NN	denotes	layer
T7746	37885-37887	IN	denotes	of
T7747	37888-37891	DT	denotes	the
T7748	37892-37901	NN	denotes	epidermis
T7749	37901-37902	.	denotes	.
T7750	37902-38133	sentence	denotes	Though there is no cell culture system available to specifically study placodal cells, these keratinocytes are their progenitors and are the closest approximation available to study the behavior of epithelial cells of the placode.
T7751	37903-37909	IN	denotes	Though
T7753	37910-37915	EX	denotes	there
T7752	37916-37918	VBZ	denotes	is
T7755	37919-37921	DT	denotes	no
T7757	37922-37926	NN	denotes	cell
T7758	37927-37934	NN	denotes	culture
T7756	37935-37941	NN	denotes	system
T7759	37942-37951	JJ	denotes	available
T7760	37952-37954	TO	denotes	to
T7762	37955-37967	RB	denotes	specifically
T7761	37968-37973	VB	denotes	study
T7763	37974-37982	JJ	denotes	placodal
T7764	37983-37988	NNS	denotes	cells
T7765	37988-37990	,	denotes	,
T7766	37990-37995	DT	denotes	these
T7767	37996-38009	NNS	denotes	keratinocytes
T7754	38010-38013	VBP	denotes	are
T7768	38014-38019	PRP$	denotes	their
T7769	38020-38031	NNS	denotes	progenitors
T7770	38032-38035	CC	denotes	and
T7771	38036-38039	VBP	denotes	are
T7772	38040-38043	DT	denotes	the
T7774	38044-38051	JJS	denotes	closest
T7773	38052-38065	NN	denotes	approximation
T7775	38066-38075	JJ	denotes	available
T7776	38076-38078	TO	denotes	to
T7777	38079-38084	VB	denotes	study
T7778	38085-38088	DT	denotes	the
T7779	38089-38097	NN	denotes	behavior
T7780	38098-38100	IN	denotes	of
T7781	38101-38111	JJ	denotes	epithelial
T7782	38112-38117	NNS	denotes	cells
T7783	38118-38120	IN	denotes	of
T7784	38121-38124	DT	denotes	the
T7785	38125-38132	NN	denotes	placode
T7786	38132-38133	.	denotes	.
T7787	38133-38281	sentence	denotes	Interestingly, treatment of cultured keratinocytes with as little as 5 ng/ml of TGF-β2 caused a rapid and transient induction of Snail (Figure 5B).
T7788	38134-38147	RB	denotes	Interestingly
T7790	38147-38149	,	denotes	,
T7791	38149-38158	NN	denotes	treatment
T7792	38159-38161	IN	denotes	of
T7793	38162-38170	VBN	denotes	cultured
T7794	38171-38184	NNS	denotes	keratinocytes
T7795	38185-38189	IN	denotes	with
T7796	38190-38192	RB	denotes	as
T7798	38193-38199	JJ	denotes	little
T7799	38200-38202	IN	denotes	as
T7797	38203-38204	CD	denotes	5
T7800	38205-38207	NN	denotes	ng
T7801	38207-38208	SYM	denotes	/
T7802	38208-38210	NN	denotes	ml
T7803	38211-38213	IN	denotes	of
T7804	38214-38217	NN	denotes	TGF
T7806	38217-38218	HYPH	denotes	-
T7805	38218-38220	NN	denotes	β2
T7789	38221-38227	VBD	denotes	caused
T7807	38228-38229	DT	denotes	a
T7809	38230-38235	JJ	denotes	rapid
T7810	38236-38239	CC	denotes	and
T7811	38240-38249	JJ	denotes	transient
T7808	38250-38259	NN	denotes	induction
T7812	38260-38262	IN	denotes	of
T7813	38263-38268	NN	denotes	Snail
T7814	38269-38270	-LRB-	denotes	(
T7816	38270-38276	NN	denotes	Figure
T7815	38277-38279	NN	denotes	5B
T7817	38279-38280	-RRB-	denotes	)
T7818	38280-38281	.	denotes	.
T7819	38281-38394	sentence	denotes	Following this treatment, Snail protein was detected within 30 min, peaked at 2 h, and then declined thereafter.
T7820	38282-38291	VBG	denotes	Following
T7822	38292-38296	DT	denotes	this
T7823	38297-38306	NN	denotes	treatment
T7824	38306-38308	,	denotes	,
T7825	38308-38313	NN	denotes	Snail
T7826	38314-38321	NN	denotes	protein
T7827	38322-38325	VBD	denotes	was
T7821	38326-38334	VBN	denotes	detected
T7828	38335-38341	IN	denotes	within
T7829	38342-38344	CD	denotes	30
T7830	38345-38348	NN	denotes	min
T7831	38348-38350	,	denotes	,
T7832	38350-38356	VBD	denotes	peaked
T7833	38357-38359	IN	denotes	at
T7834	38360-38361	CD	denotes	2
T7835	38362-38363	NN	denotes	h
T7836	38363-38365	,	denotes	,
T7837	38365-38368	CC	denotes	and
T7838	38369-38373	RB	denotes	then
T7839	38374-38382	VBD	denotes	declined
T7840	38383-38393	RB	denotes	thereafter
T7841	38393-38394	.	denotes	.
T7842	38394-38586	sentence	denotes	The induction of Snail appeared to be specific for the active form of the growth factor, as pretreatment of TGF-β2 for 10 min at 100 °C obliterated the response [Figure 5B, lanes marked (–)].
T7843	38395-38398	DT	denotes	The
T7844	38399-38408	NN	denotes	induction
T7846	38409-38411	IN	denotes	of
T7847	38412-38417	NN	denotes	Snail
T7845	38418-38426	VBD	denotes	appeared
T7848	38427-38429	TO	denotes	to
T7849	38430-38432	VB	denotes	be
T7850	38433-38441	JJ	denotes	specific
T7851	38442-38445	IN	denotes	for
T7852	38446-38449	DT	denotes	the
T7854	38450-38456	JJ	denotes	active
T7853	38457-38461	NN	denotes	form
T7855	38462-38464	IN	denotes	of
T7856	38465-38468	DT	denotes	the
T7858	38469-38475	NN	denotes	growth
T7857	38476-38482	NN	denotes	factor
T7859	38482-38484	,	denotes	,
T7860	38484-38486	IN	denotes	as
T7862	38487-38499	NN	denotes	pretreatment
T7863	38500-38502	IN	denotes	of
T7864	38503-38506	NN	denotes	TGF
T7866	38506-38507	HYPH	denotes	-
T7865	38507-38509	NN	denotes	β2
T7867	38510-38513	IN	denotes	for
T7868	38514-38516	CD	denotes	10
T7869	38517-38520	NN	denotes	min
T7870	38521-38523	IN	denotes	at
T7871	38524-38527	CD	denotes	100
T7872	38528-38530	NN	denotes	°C
T7861	38531-38542	VBD	denotes	obliterated
T7873	38543-38546	DT	denotes	the
T7874	38547-38555	NN	denotes	response
T7875	38556-38557	-LRB-	denotes	[
T7877	38557-38563	NN	denotes	Figure
T7878	38564-38566	NN	denotes	5B
T7879	38566-38568	,	denotes	,
T7876	38568-38573	NNS	denotes	lanes
T7880	38574-38580	VBN	denotes	marked
T7881	38581-38582	-LRB-	denotes	(
T7882	38582-38583	SYM	denotes	–
T7883	38583-38584	-RRB-	denotes	)
T7884	38584-38585	-RRB-	denotes	]
T7885	38585-38586	.	denotes	.
T7886	38586-38824	sentence	denotes	By contrast, although TGF-β receptor activation remained elevated during the duration of the experiment (as measured by the sustained phosphorylation of the downstream effector SMAD2) Snail expression could not be maintained (Figure 5B).
T7887	38587-38589	IN	denotes	By
T7889	38590-38598	NN	denotes	contrast
T7890	38598-38600	,	denotes	,
T7891	38600-38608	IN	denotes	although
T7893	38609-38612	NN	denotes	TGF
T7895	38612-38613	HYPH	denotes	-
T7894	38613-38614	NN	denotes	β
T7896	38615-38623	NN	denotes	receptor
T7897	38624-38634	NN	denotes	activation
T7892	38635-38643	VBD	denotes	remained
T7898	38644-38652	JJ	denotes	elevated
T7899	38653-38659	IN	denotes	during
T7900	38660-38663	DT	denotes	the
T7901	38664-38672	NN	denotes	duration
T7902	38673-38675	IN	denotes	of
T7903	38676-38679	DT	denotes	the
T7904	38680-38690	NN	denotes	experiment
T7905	38691-38692	-LRB-	denotes	(
T7906	38692-38694	IN	denotes	as
T7907	38695-38703	VBN	denotes	measured
T7908	38704-38706	IN	denotes	by
T7909	38707-38710	DT	denotes	the
T7911	38711-38720	JJ	denotes	sustained
T7910	38721-38736	NN	denotes	phosphorylation
T7912	38737-38739	IN	denotes	of
T7913	38740-38743	DT	denotes	the
T7915	38744-38754	JJ	denotes	downstream
T7914	38755-38763	NN	denotes	effector
T7916	38764-38769	NN	denotes	SMAD2
T7917	38769-38770	-RRB-	denotes	)
T7918	38771-38776	NN	denotes	Snail
T7919	38777-38787	NN	denotes	expression
T7920	38788-38793	MD	denotes	could
T7921	38794-38797	RB	denotes	not
T7922	38798-38800	VB	denotes	be
T7888	38801-38811	VBN	denotes	maintained
T7923	38812-38813	-LRB-	denotes	(
T7925	38813-38819	NN	denotes	Figure
T7924	38820-38822	NN	denotes	5B
T7926	38822-38823	-RRB-	denotes	)
T7927	38823-38824	.	denotes	.
T7928	38824-38972	sentence	denotes	Thus, although Snail expression correlated with phosphorylated SMAD2 (pSMAD2) induction, its decline seemed to rely on secondary downstream events.
T7929	38825-38829	RB	denotes	Thus
T7931	38829-38831	,	denotes	,
T7932	38831-38839	IN	denotes	although
T7934	38840-38845	NN	denotes	Snail
T7935	38846-38856	NN	denotes	expression
T7933	38857-38867	VBD	denotes	correlated
T7936	38868-38872	IN	denotes	with
T7937	38873-38887	VBN	denotes	phosphorylated
T7938	38888-38893	NN	denotes	SMAD2
T7940	38894-38895	-LRB-	denotes	(
T7941	38895-38901	NN	denotes	pSMAD2
T7942	38901-38902	-RRB-	denotes	)
T7939	38903-38912	NN	denotes	induction
T7943	38912-38914	,	denotes	,
T7944	38914-38917	PRP$	denotes	its
T7945	38918-38925	NN	denotes	decline
T7930	38926-38932	VBD	denotes	seemed
T7946	38933-38935	TO	denotes	to
T7947	38936-38940	VB	denotes	rely
T7948	38941-38943	IN	denotes	on
T7949	38944-38953	JJ	denotes	secondary
T7951	38954-38964	JJ	denotes	downstream
T7950	38965-38971	NNS	denotes	events
T7952	38971-38972	.	denotes	.
T7953	38972-39154	sentence	denotes	The rapid kinetics of Snail expression were reflected at the mRNA level, suggesting that Snail promoter activity in keratinocytes might be sensitive to TGF-β2 signaling (Figure 5C).
T7954	38973-38976	DT	denotes	The
T7956	38977-38982	JJ	denotes	rapid
T7955	38983-38991	NNS	denotes	kinetics
T7958	38992-38994	IN	denotes	of
T7959	38995-39000	NN	denotes	Snail
T7960	39001-39011	NN	denotes	expression
T7961	39012-39016	VBD	denotes	were
T7957	39017-39026	VBN	denotes	reflected
T7962	39027-39029	IN	denotes	at
T7963	39030-39033	DT	denotes	the
T7965	39034-39038	NN	denotes	mRNA
T7964	39039-39044	NN	denotes	level
T7966	39044-39046	,	denotes	,
T7967	39046-39056	VBG	denotes	suggesting
T7968	39057-39061	IN	denotes	that
T7970	39062-39067	NN	denotes	Snail
T7971	39068-39076	NN	denotes	promoter
T7972	39077-39085	NN	denotes	activity
T7973	39086-39088	IN	denotes	in
T7974	39089-39102	NNS	denotes	keratinocytes
T7975	39103-39108	MD	denotes	might
T7969	39109-39111	VB	denotes	be
T7976	39112-39121	JJ	denotes	sensitive
T7977	39122-39124	IN	denotes	to
T7978	39125-39128	NN	denotes	TGF
T7980	39128-39129	HYPH	denotes	-
T7979	39129-39131	NN	denotes	β2
T7981	39132-39141	NN	denotes	signaling
T7982	39142-39143	-LRB-	denotes	(
T7984	39143-39149	NN	denotes	Figure
T7983	39150-39152	NN	denotes	5C
T7985	39152-39153	-RRB-	denotes	)
T7986	39153-39154	.	denotes	.
T7987	39154-39381	sentence	denotes	To test this possibility, we engineered a transgene driving the β-galactosidase reporter under the control of approximately 2.2 kb of promoter sequence located 5′ from the transcription initiation site of the mouse Snail gene.
T7988	39155-39157	TO	denotes	To
T7989	39158-39162	VB	denotes	test
T7991	39163-39167	DT	denotes	this
T7992	39168-39179	NN	denotes	possibility
T7993	39179-39181	,	denotes	,
T7994	39181-39183	PRP	denotes	we
T7990	39184-39194	VBD	denotes	engineered
T7995	39195-39196	DT	denotes	a
T7996	39197-39206	NN	denotes	transgene
T7997	39207-39214	VBG	denotes	driving
T7998	39215-39218	DT	denotes	the
T8000	39219-39220	NN	denotes	β
T8002	39220-39221	HYPH	denotes	-
T8001	39221-39234	NN	denotes	galactosidase
T7999	39235-39243	NN	denotes	reporter
T8003	39244-39249	IN	denotes	under
T8004	39250-39253	DT	denotes	the
T8005	39254-39261	NN	denotes	control
T8006	39262-39264	IN	denotes	of
T8007	39265-39278	RB	denotes	approximately
T8008	39279-39282	CD	denotes	2.2
T8009	39283-39285	NN	denotes	kb
T8010	39286-39288	IN	denotes	of
T8011	39289-39297	NN	denotes	promoter
T8012	39298-39306	NN	denotes	sequence
T8013	39307-39314	VBN	denotes	located
T8014	39315-39316	CD	denotes	5
T8015	39316-39317	SYM	denotes	′
T8016	39318-39322	IN	denotes	from
T8017	39323-39326	DT	denotes	the
T8019	39327-39340	NN	denotes	transcription
T8020	39341-39351	NN	denotes	initiation
T8018	39352-39356	NN	denotes	site
T8021	39357-39359	IN	denotes	of
T8022	39360-39363	DT	denotes	the
T8024	39364-39369	NN	denotes	mouse
T8025	39370-39375	NN	denotes	Snail
T8023	39376-39380	NN	denotes	gene
T8026	39380-39381	.	denotes	.
T8027	39381-39581	sentence	denotes	At 2 d after transient transfection, keratinocytes were treated with TGF-β2 (t = 0) and then assayed for transgene activity over the same time course in which we had observed Snail protein induction.
T8028	39382-39384	IN	denotes	At
T8030	39385-39386	CD	denotes	2
T8031	39387-39388	NN	denotes	d
T8032	39389-39394	IN	denotes	after
T8033	39395-39404	JJ	denotes	transient
T8034	39405-39417	NN	denotes	transfection
T8035	39417-39419	,	denotes	,
T8036	39419-39432	NNS	denotes	keratinocytes
T8037	39433-39437	VBD	denotes	were
T8029	39438-39445	VBN	denotes	treated
T8038	39446-39450	IN	denotes	with
T8039	39451-39454	NN	denotes	TGF
T8041	39454-39455	HYPH	denotes	-
T8040	39455-39457	NN	denotes	β2
T8042	39458-39459	-LRB-	denotes	(
T8044	39459-39460	NN	denotes	t
T8045	39461-39462	SYM	denotes	=
T8043	39463-39464	CD	denotes	0
T8046	39464-39465	-RRB-	denotes	)
T8047	39466-39469	CC	denotes	and
T8048	39470-39474	RB	denotes	then
T8049	39475-39482	VBN	denotes	assayed
T8050	39483-39486	IN	denotes	for
T8051	39487-39496	NN	denotes	transgene
T8052	39497-39505	NN	denotes	activity
T8053	39506-39510	IN	denotes	over
T8054	39511-39514	DT	denotes	the
T8056	39515-39519	JJ	denotes	same
T8057	39520-39524	NN	denotes	time
T8055	39525-39531	NN	denotes	course
T8058	39532-39534	IN	denotes	in
T8060	39535-39540	WDT	denotes	which
T8061	39541-39543	PRP	denotes	we
T8062	39544-39547	VBD	denotes	had
T8059	39548-39556	VBN	denotes	observed
T8063	39557-39562	NN	denotes	Snail
T8064	39563-39570	NN	denotes	protein
T8065	39571-39580	NN	denotes	induction
T8066	39580-39581	.	denotes	.
T8067	39581-39640	sentence	denotes	The results of this experiment are presented in Figure 5D.
T8068	39582-39585	DT	denotes	The
T8069	39586-39593	NNS	denotes	results
T8071	39594-39596	IN	denotes	of
T8072	39597-39601	DT	denotes	this
T8073	39602-39612	NN	denotes	experiment
T8074	39613-39616	VBP	denotes	are
T8070	39617-39626	VBN	denotes	presented
T8075	39627-39629	IN	denotes	in
T8076	39630-39636	NN	denotes	Figure
T8077	39637-39639	NN	denotes	5D
T8078	39639-39640	.	denotes	.
T8079	39640-39800	sentence	denotes	Within 0.5 h of TGF-β2 treatment, Snail promoter activity had increased 3-fold, and by 2 h, it peaked to approximately 10-fold over control levels (Figure 5D).
T8080	39641-39647	IN	denotes	Within
T8082	39648-39651	CD	denotes	0.5
T8083	39652-39653	NN	denotes	h
T8084	39654-39656	IN	denotes	of
T8085	39657-39660	NN	denotes	TGF
T8087	39660-39661	HYPH	denotes	-
T8086	39661-39663	NN	denotes	β2
T8088	39664-39673	NN	denotes	treatment
T8089	39673-39675	,	denotes	,
T8090	39675-39680	NN	denotes	Snail
T8091	39681-39689	NN	denotes	promoter
T8092	39690-39698	NN	denotes	activity
T8093	39699-39702	VBD	denotes	had
T8081	39703-39712	VBN	denotes	increased
T8094	39713-39719	RB	denotes	3-fold
T8095	39719-39721	,	denotes	,
T8096	39721-39724	CC	denotes	and
T8097	39725-39727	IN	denotes	by
T8099	39728-39729	CD	denotes	2
T8100	39730-39731	NN	denotes	h
T8101	39731-39733	,	denotes	,
T8102	39733-39735	PRP	denotes	it
T8098	39736-39742	VBD	denotes	peaked
T8103	39743-39745	IN	denotes	to
T8104	39746-39759	RB	denotes	approximately
T8105	39760-39767	RB	denotes	10-fold
T8106	39768-39772	IN	denotes	over
T8107	39773-39780	NN	denotes	control
T8108	39781-39787	NNS	denotes	levels
T8109	39788-39789	-LRB-	denotes	(
T8111	39789-39795	NN	denotes	Figure
T8110	39796-39798	NN	denotes	5D
T8112	39798-39799	-RRB-	denotes	)
T8113	39799-39800	.	denotes	.
T8114	39800-39909	sentence	denotes	Thereafter, Snail promoter activity rapidly returned to the basal levels seen in unstimulated keratinocytes.
T8115	39801-39811	RB	denotes	Thereafter
T8117	39811-39813	,	denotes	,
T8118	39813-39818	NN	denotes	Snail
T8119	39819-39827	NN	denotes	promoter
T8120	39828-39836	NN	denotes	activity
T8121	39837-39844	RB	denotes	rapidly
T8116	39845-39853	VBD	denotes	returned
T8122	39854-39856	IN	denotes	to
T8123	39857-39860	DT	denotes	the
T8125	39861-39866	JJ	denotes	basal
T8124	39867-39873	NNS	denotes	levels
T8126	39874-39878	VBN	denotes	seen
T8127	39879-39881	IN	denotes	in
T8128	39882-39894	JJ	denotes	unstimulated
T8129	39895-39908	NNS	denotes	keratinocytes
T8130	39908-39909	.	denotes	.
T8131	39909-40012	sentence	denotes	The kinetics of Snail promoter activity closely paralleled those observed for Snail protein induction.
T8132	39910-39913	DT	denotes	The
T8133	39914-39922	NNS	denotes	kinetics
T8135	39923-39925	IN	denotes	of
T8136	39926-39931	NN	denotes	Snail
T8137	39932-39940	NN	denotes	promoter
T8138	39941-39949	NN	denotes	activity
T8139	39950-39957	RB	denotes	closely
T8134	39958-39968	VBD	denotes	paralleled
T8140	39969-39974	DT	denotes	those
T8141	39975-39983	VBN	denotes	observed
T8142	39984-39987	IN	denotes	for
T8143	39988-39993	NN	denotes	Snail
T8144	39994-40001	NN	denotes	protein
T8145	40002-40011	NN	denotes	induction
T8146	40011-40012	.	denotes	.
T8147	40012-40284	sentence	denotes	Moreover, the stimulatory effects appeared to be specific to TGF-β2, since they were abrogated either by heat inactivation of the TGF-β2 protein or by mutation of a putative SMAD binding element located about 1.8 kb 5′ from the Snail transcription start site (Figure 5D).
T8148	40013-40021	RB	denotes	Moreover
T8150	40021-40023	,	denotes	,
T8151	40023-40026	DT	denotes	the
T8153	40027-40038	JJ	denotes	stimulatory
T8152	40039-40046	NNS	denotes	effects
T8149	40047-40055	VBD	denotes	appeared
T8154	40056-40058	TO	denotes	to
T8155	40059-40061	VB	denotes	be
T8156	40062-40070	JJ	denotes	specific
T8157	40071-40073	IN	denotes	to
T8158	40074-40077	NN	denotes	TGF
T8160	40077-40078	HYPH	denotes	-
T8159	40078-40080	NN	denotes	β2
T8161	40080-40082	,	denotes	,
T8162	40082-40087	IN	denotes	since
T8164	40088-40092	PRP	denotes	they
T8165	40093-40097	VBD	denotes	were
T8163	40098-40107	VBN	denotes	abrogated
T8166	40108-40114	CC	denotes	either
T8167	40115-40117	IN	denotes	by
T8168	40118-40122	NN	denotes	heat
T8169	40123-40135	NN	denotes	inactivation
T8170	40136-40138	IN	denotes	of
T8171	40139-40142	DT	denotes	the
T8173	40143-40146	NN	denotes	TGF
T8175	40146-40147	HYPH	denotes	-
T8174	40147-40149	NN	denotes	β2
T8172	40150-40157	NN	denotes	protein
T8176	40158-40160	CC	denotes	or
T8177	40161-40163	IN	denotes	by
T8178	40164-40172	NN	denotes	mutation
T8179	40173-40175	IN	denotes	of
T8180	40176-40177	DT	denotes	a
T8182	40178-40186	JJ	denotes	putative
T8183	40187-40191	NN	denotes	SMAD
T8184	40192-40199	NN	denotes	binding
T8181	40200-40207	NN	denotes	element
T8185	40208-40215	VBN	denotes	located
T8186	40216-40221	IN	denotes	about
T8187	40222-40225	CD	denotes	1.8
T8188	40226-40228	NN	denotes	kb
T8189	40229-40230	CD	denotes	5
T8190	40230-40231	SYM	denotes	′
T8191	40232-40236	IN	denotes	from
T8192	40237-40240	DT	denotes	the
T8194	40241-40246	NN	denotes	Snail
T8195	40247-40260	NN	denotes	transcription
T8196	40261-40266	NN	denotes	start
T8193	40267-40271	NN	denotes	site
T8197	40272-40273	-LRB-	denotes	(
T8199	40273-40279	NN	denotes	Figure
T8198	40280-40282	NN	denotes	5D
T8200	40282-40283	-RRB-	denotes	)
T8201	40283-40284	.	denotes	.
T8202	40284-40523	sentence	denotes	Taken together, these results suggested that in keratinocytes, TGF-β2 signaling results in a pSMAD2-dependent transient activation of the Snail gene, and that maintenance of Snail protein relies, in part, upon sustained promoter activity.
T8203	40285-40290	VBN	denotes	Taken
T8205	40291-40299	RB	denotes	together
T8206	40299-40301	,	denotes	,
T8207	40301-40306	DT	denotes	these
T8208	40307-40314	NNS	denotes	results
T8204	40315-40324	VBD	denotes	suggested
T8209	40325-40329	IN	denotes	that
T8211	40330-40332	IN	denotes	in
T8212	40333-40346	NNS	denotes	keratinocytes
T8213	40346-40348	,	denotes	,
T8214	40348-40351	NN	denotes	TGF
T8216	40351-40352	HYPH	denotes	-
T8215	40352-40354	NN	denotes	β2
T8217	40355-40364	NN	denotes	signaling
T8210	40365-40372	VBZ	denotes	results
T8218	40373-40375	IN	denotes	in
T8219	40376-40377	DT	denotes	a
T8221	40378-40384	NN	denotes	pSMAD2
T8223	40384-40385	HYPH	denotes	-
T8222	40385-40394	JJ	denotes	dependent
T8224	40395-40404	JJ	denotes	transient
T8220	40405-40415	NN	denotes	activation
T8225	40416-40418	IN	denotes	of
T8226	40419-40422	DT	denotes	the
T8228	40423-40428	NN	denotes	Snail
T8227	40429-40433	NN	denotes	gene
T8229	40433-40435	,	denotes	,
T8230	40435-40438	CC	denotes	and
T8231	40439-40443	IN	denotes	that
T8233	40444-40455	NN	denotes	maintenance
T8234	40456-40458	IN	denotes	of
T8235	40459-40464	NN	denotes	Snail
T8236	40465-40472	NN	denotes	protein
T8232	40473-40479	VBZ	denotes	relies
T8237	40479-40481	,	denotes	,
T8238	40481-40483	IN	denotes	in
T8239	40484-40488	NN	denotes	part
T8240	40488-40490	,	denotes	,
T8241	40490-40494	IN	denotes	upon
T8242	40495-40504	JJ	denotes	sustained
T8244	40505-40513	NN	denotes	promoter
T8243	40514-40522	NN	denotes	activity
T8245	40522-40523	.	denotes	.
T8246	40523-40746	sentence	denotes	The brevity of Snail gene and protein induction in TGF-β2 treated cultured keratinocytes resembled the temporal appearance of Snail mRNA and protein at the initiation of hair follicle morphogenesis in embryonic mouse skin.
T8247	40524-40527	DT	denotes	The
T8248	40528-40535	NN	denotes	brevity
T8250	40536-40538	IN	denotes	of
T8251	40539-40544	NN	denotes	Snail
T8252	40545-40549	NN	denotes	gene
T8254	40550-40553	CC	denotes	and
T8255	40554-40561	NN	denotes	protein
T8253	40562-40571	NN	denotes	induction
T8256	40572-40574	IN	denotes	in
T8257	40575-40578	NN	denotes	TGF
T8259	40578-40579	HYPH	denotes	-
T8258	40579-40581	NN	denotes	β2
T8260	40582-40589	VBN	denotes	treated
T8262	40590-40598	VBN	denotes	cultured
T8261	40599-40612	NNS	denotes	keratinocytes
T8249	40613-40622	VBD	denotes	resembled
T8263	40623-40626	DT	denotes	the
T8265	40627-40635	JJ	denotes	temporal
T8264	40636-40646	NN	denotes	appearance
T8266	40647-40649	IN	denotes	of
T8267	40650-40655	NN	denotes	Snail
T8268	40656-40660	NN	denotes	mRNA
T8269	40661-40664	CC	denotes	and
T8270	40665-40672	NN	denotes	protein
T8271	40673-40675	IN	denotes	at
T8272	40676-40679	DT	denotes	the
T8273	40680-40690	NN	denotes	initiation
T8274	40691-40693	IN	denotes	of
T8275	40694-40698	NN	denotes	hair
T8276	40699-40707	NN	denotes	follicle
T8277	40708-40721	NN	denotes	morphogenesis
T8278	40722-40724	IN	denotes	in
T8279	40725-40734	JJ	denotes	embryonic
T8281	40735-40740	NN	denotes	mouse
T8280	40741-40745	NN	denotes	skin
T8282	40745-40746	.	denotes	.
T8283	40746-40912	sentence	denotes	To test whether TGF-β2 might be required for Snail induction in hair bud formation in vivo, we first analyzed whether TGF-β2 was expressed in or around the hair bud.
T8284	40747-40749	TO	denotes	To
T8285	40750-40754	VB	denotes	test
T8287	40755-40762	IN	denotes	whether
T8289	40763-40766	NN	denotes	TGF
T8291	40766-40767	HYPH	denotes	-
T8290	40767-40769	NN	denotes	β2
T8292	40770-40775	MD	denotes	might
T8293	40776-40778	VB	denotes	be
T8288	40779-40787	VBN	denotes	required
T8294	40788-40791	IN	denotes	for
T8295	40792-40797	NN	denotes	Snail
T8296	40798-40807	NN	denotes	induction
T8297	40808-40810	IN	denotes	in
T8298	40811-40815	NN	denotes	hair
T8299	40816-40819	NN	denotes	bud
T8300	40820-40829	NN	denotes	formation
T8301	40830-40832	FW	denotes	in
T8302	40833-40837	FW	denotes	vivo
T8303	40837-40839	,	denotes	,
T8304	40839-40841	PRP	denotes	we
T8305	40842-40847	RB	denotes	first
T8286	40848-40856	VBD	denotes	analyzed
T8306	40857-40864	IN	denotes	whether
T8308	40865-40868	NN	denotes	TGF
T8310	40868-40869	HYPH	denotes	-
T8309	40869-40871	NN	denotes	β2
T8311	40872-40875	VBD	denotes	was
T8307	40876-40885	VBN	denotes	expressed
T8312	40886-40888	IN	denotes	in
T8313	40889-40891	CC	denotes	or
T8314	40892-40898	IN	denotes	around
T8315	40899-40902	DT	denotes	the
T8317	40903-40907	NN	denotes	hair
T8316	40908-40911	NN	denotes	bud
T8318	40911-40912	.	denotes	.
T8319	40912-41022	sentence	denotes	Consistent with previous observations [33], an anti-TGF-β2 antibody labeled developing hair buds (Figure 6A).
T8320	40913-40923	JJ	denotes	Consistent
T8322	40924-40928	IN	denotes	with
T8323	40929-40937	JJ	denotes	previous
T8324	40938-40950	NNS	denotes	observations
T8325	40951-40952	-LRB-	denotes	[
T8326	40952-40954	CD	denotes	33
T8327	40954-40955	-RRB-	denotes	]
T8328	40955-40957	,	denotes	,
T8329	40957-40959	DT	denotes	an
T8331	40960-40968	JJ	denotes	anti-TGF
T8333	40968-40969	HYPH	denotes	-
T8332	40969-40971	NN	denotes	β2
T8330	40972-40980	NN	denotes	antibody
T8321	40981-40988	VBD	denotes	labeled
T8334	40989-40999	VBG	denotes	developing
T8336	41000-41004	NN	denotes	hair
T8335	41005-41009	NNS	denotes	buds
T8337	41010-41011	-LRB-	denotes	(
T8339	41011-41017	NN	denotes	Figure
T8338	41018-41020	NN	denotes	6A
T8340	41020-41021	-RRB-	denotes	)
T8341	41021-41022	.	denotes	.
T8342	41022-41178	sentence	denotes	This labeling appeared to be specific as judged by the lack of staining in follicle buds from mice homozygous for a TGF-β2 null mutation (Figure 6A; [34]).
T8343	41023-41027	DT	denotes	This
T8344	41028-41036	NN	denotes	labeling
T8345	41037-41045	VBD	denotes	appeared
T8346	41046-41048	TO	denotes	to
T8347	41049-41051	VB	denotes	be
T8348	41052-41060	JJ	denotes	specific
T8349	41061-41063	IN	denotes	as
T8350	41064-41070	VBN	denotes	judged
T8351	41071-41073	IN	denotes	by
T8352	41074-41077	DT	denotes	the
T8353	41078-41082	NN	denotes	lack
T8354	41083-41085	IN	denotes	of
T8355	41086-41094	NN	denotes	staining
T8356	41095-41097	IN	denotes	in
T8357	41098-41106	NN	denotes	follicle
T8358	41107-41111	NNS	denotes	buds
T8359	41112-41116	IN	denotes	from
T8360	41117-41121	NNS	denotes	mice
T8361	41122-41132	JJ	denotes	homozygous
T8362	41133-41136	IN	denotes	for
T8363	41137-41138	DT	denotes	a
T8365	41139-41142	NN	denotes	TGF
T8367	41142-41143	HYPH	denotes	-
T8366	41143-41145	NN	denotes	β2
T8368	41146-41150	JJ	denotes	null
T8364	41151-41159	NN	denotes	mutation
T8369	41160-41161	-LRB-	denotes	(
T8371	41161-41167	NN	denotes	Figure
T8372	41168-41170	NN	denotes	6A
T8373	41170-41171	:	denotes	;
T8374	41172-41173	-LRB-	denotes	[
T8370	41173-41175	CD	denotes	34
T8375	41175-41176	-RRB-	denotes	]
T8376	41176-41177	-RRB-	denotes	)
T8377	41177-41178	.	denotes	.
T8378	41178-41311	sentence	denotes	Moreover, the downstream effector of TGF-β2 signaling, pSMAD2, was also expressed in WT, but not TGF-β2-null, hair buds (Figure 6B).
T8379	41179-41187	RB	denotes	Moreover
T8381	41187-41189	,	denotes	,
T8382	41189-41192	DT	denotes	the
T8384	41193-41203	JJ	denotes	downstream
T8383	41204-41212	NN	denotes	effector
T8385	41213-41215	IN	denotes	of
T8386	41216-41219	NN	denotes	TGF
T8388	41219-41220	HYPH	denotes	-
T8387	41220-41222	NN	denotes	β2
T8389	41223-41232	NN	denotes	signaling
T8390	41232-41234	,	denotes	,
T8391	41234-41240	NN	denotes	pSMAD2
T8392	41240-41242	,	denotes	,
T8393	41242-41245	VBD	denotes	was
T8394	41246-41250	RB	denotes	also
T8380	41251-41260	VBN	denotes	expressed
T8395	41261-41263	IN	denotes	in
T8396	41264-41266	NN	denotes	WT
T8398	41266-41268	,	denotes	,
T8399	41268-41271	CC	denotes	but
T8400	41272-41275	RB	denotes	not
T8401	41276-41279	NN	denotes	TGF
T8403	41279-41280	HYPH	denotes	-
T8402	41280-41282	NN	denotes	β2
T8405	41282-41283	HYPH	denotes	-
T8404	41283-41287	JJ	denotes	null
T8406	41287-41289	,	denotes	,
T8407	41289-41293	NN	denotes	hair
T8397	41294-41298	NNS	denotes	buds
T8408	41299-41300	-LRB-	denotes	(
T8410	41300-41306	NN	denotes	Figure
T8409	41307-41309	NN	denotes	6B
T8411	41309-41310	-RRB-	denotes	)
T8412	41310-41311	.	denotes	.
T8413	41311-41464	sentence	denotes	Together, these data underscore the importance of the TGF-β2 isoform despite expression of both TGF-β1 and TGF-β2 in developing hair buds at this stage.
T8414	41312-41320	RB	denotes	Together
T8416	41320-41322	,	denotes	,
T8417	41322-41327	DT	denotes	these
T8418	41328-41332	NNS	denotes	data
T8415	41333-41343	VBP	denotes	underscore
T8419	41344-41347	DT	denotes	the
T8420	41348-41358	NN	denotes	importance
T8421	41359-41361	IN	denotes	of
T8422	41362-41365	DT	denotes	the
T8424	41366-41369	NN	denotes	TGF
T8426	41369-41370	HYPH	denotes	-
T8425	41370-41372	NN	denotes	β2
T8423	41373-41380	NN	denotes	isoform
T8427	41381-41388	IN	denotes	despite
T8428	41389-41399	NN	denotes	expression
T8429	41400-41402	IN	denotes	of
T8430	41403-41407	CC	denotes	both
T8432	41408-41411	NN	denotes	TGF
T8433	41411-41412	HYPH	denotes	-
T8431	41412-41414	NN	denotes	β1
T8434	41415-41418	CC	denotes	and
T8435	41419-41422	NN	denotes	TGF
T8437	41422-41423	HYPH	denotes	-
T8436	41423-41425	NN	denotes	β2
T8438	41426-41428	IN	denotes	in
T8439	41429-41439	VBG	denotes	developing
T8441	41440-41444	NN	denotes	hair
T8440	41445-41449	NNS	denotes	buds
T8442	41450-41452	IN	denotes	at
T8443	41453-41457	DT	denotes	this
T8444	41458-41463	NN	denotes	stage
T8445	41463-41464	.	denotes	.
T8446	41464-42595	sentence	denotes	Figure 6 TGF-β2 Is Necessary to Induce Snail Expression and Regulate Proliferation and E-Cadherin in the Hair Bud (A–D) Skins from TGF-β2 WT or KO E17.5 embryos were analyzed for expression of TGF-β2 protein (A), which is present in the epidermis and dermis as previously described [33] and in the hair bud, pSMAD2 (B), Snail (C), and Snail mRNA (D). Arrows point to the hair buds. (E) Western blot analyses of Snail expression in the skins of 2-wk-old K14-Smad2 transgenic (SMAD2 TG) and WT littermate (WT) mice. Antibody to tubulin was used as a control for equal protein loadings. The K14-Smad2 Tg mouse was previously shown to possess activated TGF-β signaling [35]. (F–G) Proliferation markers Ki67 (F) and pMAPK (G) are diminished in TGF-β2-null hair relative to its WT counterpart. (H–J) TGF-β2-null hair fails to down-regulate E-cadherin (H). Wnt and noggin signaling pathways are still intact in the TGF-β2 null hair as nuclear LEF-1 (I) and nuclear β-catenin (J) are still expressed. To further explore the possible relation between Snail and TGF-β2, we examined the status of Snail expression in TGF-β2-null hair buds.
T8447	42460-42462	TO	denotes	To
T8449	42463-42470	RB	denotes	further
T8448	42471-42478	VB	denotes	explore
T8451	42479-42482	DT	denotes	the
T8453	42483-42491	JJ	denotes	possible
T8452	42492-42500	NN	denotes	relation
T8454	42501-42508	IN	denotes	between
T8455	42509-42514	NN	denotes	Snail
T8456	42515-42518	CC	denotes	and
T8457	42519-42522	NN	denotes	TGF
T8459	42522-42523	HYPH	denotes	-
T8458	42523-42525	NN	denotes	β2
T8460	42525-42527	,	denotes	,
T8461	42527-42529	PRP	denotes	we
T8450	42530-42538	VBD	denotes	examined
T8462	42539-42542	DT	denotes	the
T8463	42543-42549	NN	denotes	status
T8464	42550-42552	IN	denotes	of
T8465	42553-42558	NN	denotes	Snail
T8466	42559-42569	NN	denotes	expression
T8467	42570-42572	IN	denotes	in
T8468	42573-42576	NN	denotes	TGF
T8470	42576-42577	HYPH	denotes	-
T8469	42577-42579	NN	denotes	β2
T8472	42579-42580	HYPH	denotes	-
T8471	42580-42584	JJ	denotes	null
T8474	42585-42589	NN	denotes	hair
T8473	42590-42594	NNS	denotes	buds
T8475	42594-42595	.	denotes	.
T8476	42595-42748	sentence	denotes	As judged by immunohistochemistry, Snail protein was absent from E17.5 skin of TGF-β2-null embryos but not from that of control littermates (Figure 6C).
T8477	42596-42598	IN	denotes	As
T8478	42599-42605	VBN	denotes	judged
T8480	42606-42608	IN	denotes	by
T8481	42609-42629	NN	denotes	immunohistochemistry
T8482	42629-42631	,	denotes	,
T8483	42631-42636	NN	denotes	Snail
T8484	42637-42644	NN	denotes	protein
T8479	42645-42648	VBD	denotes	was
T8485	42649-42655	JJ	denotes	absent
T8486	42656-42660	IN	denotes	from
T8487	42661-42666	NN	denotes	E17.5
T8488	42667-42671	NN	denotes	skin
T8489	42672-42674	IN	denotes	of
T8490	42675-42678	NN	denotes	TGF
T8492	42678-42679	HYPH	denotes	-
T8491	42679-42681	NN	denotes	β2
T8494	42681-42682	HYPH	denotes	-
T8493	42682-42686	JJ	denotes	null
T8495	42687-42694	NNS	denotes	embryos
T8496	42695-42698	CC	denotes	but
T8497	42699-42702	RB	denotes	not
T8498	42703-42707	IN	denotes	from
T8499	42708-42712	DT	denotes	that
T8500	42713-42715	IN	denotes	of
T8501	42716-42723	NN	denotes	control
T8502	42724-42735	NNS	denotes	littermates
T8503	42736-42737	-LRB-	denotes	(
T8505	42737-42743	NN	denotes	Figure
T8504	42744-42746	NN	denotes	6C
T8506	42746-42747	-RRB-	denotes	)
T8507	42747-42748	.	denotes	.
T8508	42748-42983	sentence	denotes	This effect appeared to be exerted at the transcriptional level, since Snail mRNAs were also not found in TGF-β2 null hair buds under conditions in which the signal was readily detected in the hair buds of littermate skin (Figure 6D).
T8509	42749-42753	DT	denotes	This
T8510	42754-42760	NN	denotes	effect
T8511	42761-42769	VBD	denotes	appeared
T8512	42770-42772	TO	denotes	to
T8514	42773-42775	VB	denotes	be
T8513	42776-42783	VBN	denotes	exerted
T8515	42784-42786	IN	denotes	at
T8516	42787-42790	DT	denotes	the
T8518	42791-42806	JJ	denotes	transcriptional
T8517	42807-42812	NN	denotes	level
T8519	42812-42814	,	denotes	,
T8520	42814-42819	IN	denotes	since
T8522	42820-42825	NN	denotes	Snail
T8523	42826-42831	NNS	denotes	mRNAs
T8524	42832-42836	VBD	denotes	were
T8525	42837-42841	RB	denotes	also
T8526	42842-42845	RB	denotes	not
T8521	42846-42851	VBN	denotes	found
T8527	42852-42854	IN	denotes	in
T8528	42855-42858	NN	denotes	TGF
T8530	42858-42859	HYPH	denotes	-
T8529	42859-42861	NN	denotes	β2
T8531	42862-42866	JJ	denotes	null
T8533	42867-42871	NN	denotes	hair
T8532	42872-42876	NNS	denotes	buds
T8534	42877-42882	IN	denotes	under
T8535	42883-42893	NNS	denotes	conditions
T8536	42894-42896	IN	denotes	in
T8538	42897-42902	WDT	denotes	which
T8539	42903-42906	DT	denotes	the
T8540	42907-42913	NN	denotes	signal
T8541	42914-42917	VBD	denotes	was
T8542	42918-42925	RB	denotes	readily
T8537	42926-42934	VBN	denotes	detected
T8543	42935-42937	IN	denotes	in
T8544	42938-42941	DT	denotes	the
T8546	42942-42946	NN	denotes	hair
T8545	42947-42951	NNS	denotes	buds
T8547	42952-42954	IN	denotes	of
T8548	42955-42965	NN	denotes	littermate
T8549	42966-42970	NN	denotes	skin
T8550	42971-42972	-LRB-	denotes	(
T8552	42972-42978	NN	denotes	Figure
T8551	42979-42981	NN	denotes	6D
T8553	42981-42982	-RRB-	denotes	)
T8554	42982-42983	.	denotes	.
T8555	42983-43194	sentence	denotes	Conversely, in 2-wk-old K14-Smad2 Tg mice, which display elevated TGF-β signaling in skin [35], Snail protein was readily detected by Western blot analyses, where it was not found in postnatal skin (Figure 6E).
T8556	42984-42994	RB	denotes	Conversely
T8558	42994-42996	,	denotes	,
T8559	42996-42998	IN	denotes	in
T8560	42999-43000	CD	denotes	2
T8562	43000-43001	HYPH	denotes	-
T8561	43001-43003	NN	denotes	wk
T8564	43003-43004	HYPH	denotes	-
T8563	43004-43007	JJ	denotes	old
T8566	43008-43011	NN	denotes	K14
T8568	43011-43012	HYPH	denotes	-
T8567	43012-43017	NN	denotes	Smad2
T8569	43018-43020	NN	denotes	Tg
T8565	43021-43025	NNS	denotes	mice
T8570	43025-43027	,	denotes	,
T8571	43027-43032	WDT	denotes	which
T8572	43033-43040	VBP	denotes	display
T8573	43041-43049	VBN	denotes	elevated
T8575	43050-43053	NN	denotes	TGF
T8577	43053-43054	HYPH	denotes	-
T8576	43054-43055	NN	denotes	β
T8574	43056-43065	NN	denotes	signaling
T8578	43066-43068	IN	denotes	in
T8579	43069-43073	NN	denotes	skin
T8580	43074-43075	-LRB-	denotes	[
T8581	43075-43077	CD	denotes	35
T8582	43077-43078	-RRB-	denotes	]
T8583	43078-43080	,	denotes	,
T8584	43080-43085	NN	denotes	Snail
T8585	43086-43093	NN	denotes	protein
T8586	43094-43097	VBD	denotes	was
T8587	43098-43105	RB	denotes	readily
T8557	43106-43114	VBN	denotes	detected
T8588	43115-43117	IN	denotes	by
T8589	43118-43125	NNP	denotes	Western
T8590	43126-43130	NN	denotes	blot
T8591	43131-43139	NNS	denotes	analyses
T8592	43139-43141	,	denotes	,
T8593	43141-43146	WRB	denotes	where
T8595	43147-43149	PRP	denotes	it
T8596	43150-43153	VBD	denotes	was
T8597	43154-43157	RB	denotes	not
T8594	43158-43163	VBN	denotes	found
T8598	43164-43166	IN	denotes	in
T8599	43167-43176	JJ	denotes	postnatal
T8600	43177-43181	NN	denotes	skin
T8601	43182-43183	-LRB-	denotes	(
T8603	43183-43189	NN	denotes	Figure
T8602	43190-43192	NN	denotes	6E
T8604	43192-43193	-RRB-	denotes	)
T8605	43193-43194	.	denotes	.
T8606	43194-43378	sentence	denotes	Taken together, these results provide compelling evidence that TGF-β2 is functionally important for inducing Snail gene expression in a pSMAD-dependent manner in developing hair buds.
T8607	43195-43200	VBN	denotes	Taken
T8609	43201-43209	RB	denotes	together
T8610	43209-43211	,	denotes	,
T8611	43211-43216	DT	denotes	these
T8612	43217-43224	NNS	denotes	results
T8608	43225-43232	VBP	denotes	provide
T8613	43233-43243	JJ	denotes	compelling
T8614	43244-43252	NN	denotes	evidence
T8615	43253-43257	IN	denotes	that
T8617	43258-43261	NN	denotes	TGF
T8619	43261-43262	HYPH	denotes	-
T8618	43262-43264	NN	denotes	β2
T8616	43265-43267	VBZ	denotes	is
T8620	43268-43280	RB	denotes	functionally
T8621	43281-43290	JJ	denotes	important
T8622	43291-43294	IN	denotes	for
T8623	43295-43303	VBG	denotes	inducing
T8624	43304-43309	NN	denotes	Snail
T8626	43310-43314	NN	denotes	gene
T8625	43315-43325	NN	denotes	expression
T8627	43326-43328	IN	denotes	in
T8628	43329-43330	DT	denotes	a
T8630	43331-43336	NN	denotes	pSMAD
T8632	43336-43337	HYPH	denotes	-
T8631	43337-43346	JJ	denotes	dependent
T8629	43347-43353	NN	denotes	manner
T8633	43354-43356	IN	denotes	in
T8634	43357-43367	VBG	denotes	developing
T8636	43368-43372	NN	denotes	hair
T8635	43373-43377	NNS	denotes	buds
T8637	43377-43378	.	denotes	.
T8638	43378-43482	sentence	denotes	Whether pMARK activity also contributes to Snail induction was not addressed in the present study [15].
T8639	43379-43386	IN	denotes	Whether
T8641	43387-43392	NN	denotes	pMARK
T8642	43393-43401	NN	denotes	activity
T8643	43402-43406	RB	denotes	also
T8640	43407-43418	VBZ	denotes	contributes
T8645	43419-43421	IN	denotes	to
T8646	43422-43427	NN	denotes	Snail
T8647	43428-43437	NN	denotes	induction
T8648	43438-43441	VBD	denotes	was
T8649	43442-43445	RB	denotes	not
T8644	43446-43455	VBN	denotes	addressed
T8650	43456-43458	IN	denotes	in
T8651	43459-43462	DT	denotes	the
T8653	43463-43470	JJ	denotes	present
T8652	43471-43476	NN	denotes	study
T8654	43477-43478	-LRB-	denotes	[
T8655	43478-43480	CD	denotes	15
T8656	43480-43481	-RRB-	denotes	]
T8657	43481-43482	.	denotes	.
T8658	43482-43592	sentence	denotes	Although some hair buds still formed in TGF-β2 null skin, their number was reduced by approximately 50% [32].
T8659	43483-43491	IN	denotes	Although
T8661	43492-43496	DT	denotes	some
T8663	43497-43501	NN	denotes	hair
T8662	43502-43506	NNS	denotes	buds
T8664	43507-43512	RB	denotes	still
T8660	43513-43519	VBD	denotes	formed
T8666	43520-43522	IN	denotes	in
T8667	43523-43526	NN	denotes	TGF
T8669	43526-43527	HYPH	denotes	-
T8668	43527-43529	NN	denotes	β2
T8670	43530-43534	JJ	denotes	null
T8671	43535-43539	NN	denotes	skin
T8672	43539-43541	,	denotes	,
T8673	43541-43546	PRP$	denotes	their
T8674	43547-43553	NN	denotes	number
T8675	43554-43557	VBD	denotes	was
T8665	43558-43565	VBN	denotes	reduced
T8676	43566-43568	IN	denotes	by
T8677	43569-43582	RB	denotes	approximately
T8678	43583-43585	CD	denotes	50
T8679	43585-43586	NN	denotes	%
T8680	43587-43588	-LRB-	denotes	[
T8681	43588-43590	CD	denotes	32
T8682	43590-43591	-RRB-	denotes	]
T8683	43591-43592	.	denotes	.
T8684	43592-43760	sentence	denotes	Thus, although the pathway mediated by TGF-β2 signaling impacts the earliest step of epithelial invagination, it does not appear to be essential for bud morphogenesis.
T8685	43593-43597	RB	denotes	Thus
T8687	43597-43599	,	denotes	,
T8688	43599-43607	IN	denotes	although
T8690	43608-43611	DT	denotes	the
T8691	43612-43619	NN	denotes	pathway
T8692	43620-43628	VBN	denotes	mediated
T8693	43629-43631	IN	denotes	by
T8694	43632-43635	NN	denotes	TGF
T8696	43635-43636	HYPH	denotes	-
T8695	43636-43638	NN	denotes	β2
T8697	43639-43648	NN	denotes	signaling
T8689	43649-43656	VBZ	denotes	impacts
T8698	43657-43660	DT	denotes	the
T8700	43661-43669	JJS	denotes	earliest
T8699	43670-43674	NN	denotes	step
T8701	43675-43677	IN	denotes	of
T8702	43678-43688	JJ	denotes	epithelial
T8703	43689-43701	NN	denotes	invagination
T8704	43701-43703	,	denotes	,
T8705	43703-43705	PRP	denotes	it
T8706	43706-43710	VBZ	denotes	does
T8707	43711-43714	RB	denotes	not
T8686	43715-43721	VB	denotes	appear
T8708	43722-43724	TO	denotes	to
T8709	43725-43727	VB	denotes	be
T8710	43728-43737	JJ	denotes	essential
T8711	43738-43741	IN	denotes	for
T8712	43742-43745	NN	denotes	bud
T8713	43746-43759	NN	denotes	morphogenesis
T8714	43759-43760	.	denotes	.
T8715	43760-44034	sentence	denotes	Consistent with this notion, basement membrane remodeling still took place in the TGF-β2-null buds, as judged by immunofluorescence with antibodies against β4 integrin, an integral component of keratinocyte-mediated adhesion to its underlying basement membrane (Figure 6F).
T8716	43761-43771	JJ	denotes	Consistent
T8718	43772-43776	IN	denotes	with
T8719	43777-43781	DT	denotes	this
T8720	43782-43788	NN	denotes	notion
T8721	43788-43790	,	denotes	,
T8722	43790-43798	NN	denotes	basement
T8723	43799-43807	NN	denotes	membrane
T8724	43808-43818	NN	denotes	remodeling
T8725	43819-43824	RB	denotes	still
T8717	43825-43829	VBD	denotes	took
T8726	43830-43835	RP	denotes	place
T8727	43836-43838	IN	denotes	in
T8728	43839-43842	DT	denotes	the
T8730	43843-43846	NN	denotes	TGF
T8732	43846-43847	HYPH	denotes	-
T8731	43847-43849	NN	denotes	β2
T8734	43849-43850	HYPH	denotes	-
T8733	43850-43854	JJ	denotes	null
T8729	43855-43859	NNS	denotes	buds
T8735	43859-43861	,	denotes	,
T8736	43861-43863	IN	denotes	as
T8737	43864-43870	VBN	denotes	judged
T8738	43871-43873	IN	denotes	by
T8739	43874-43892	NN	denotes	immunofluorescence
T8740	43893-43897	IN	denotes	with
T8741	43898-43908	NNS	denotes	antibodies
T8742	43909-43916	IN	denotes	against
T8743	43917-43919	NN	denotes	β4
T8744	43920-43928	NN	denotes	integrin
T8745	43928-43930	,	denotes	,
T8746	43930-43932	DT	denotes	an
T8748	43933-43941	JJ	denotes	integral
T8747	43942-43951	NN	denotes	component
T8749	43952-43954	IN	denotes	of
T8750	43955-43967	NN	denotes	keratinocyte
T8752	43967-43968	HYPH	denotes	-
T8751	43968-43976	VBN	denotes	mediated
T8753	43977-43985	NN	denotes	adhesion
T8754	43986-43988	IN	denotes	to
T8755	43989-43992	PRP$	denotes	its
T8757	43993-44003	JJ	denotes	underlying
T8758	44004-44012	NN	denotes	basement
T8756	44013-44021	NN	denotes	membrane
T8759	44022-44023	-LRB-	denotes	(
T8761	44023-44029	NN	denotes	Figure
T8760	44030-44032	NN	denotes	6F
T8762	44032-44033	-RRB-	denotes	)
T8763	44033-44034	.	denotes	.
T8764	44034-44245	sentence	denotes	In contrast, TGF-β2 signaling appeared to be an important factor for the early proliferation that occurs in the developing hair buds, as judged by anti-Ki67 and anti-pMAPK immunofluorescence (Figure 6F and 6G).
T8765	44035-44037	IN	denotes	In
T8767	44038-44046	NN	denotes	contrast
T8768	44046-44048	,	denotes	,
T8769	44048-44051	NN	denotes	TGF
T8771	44051-44052	HYPH	denotes	-
T8770	44052-44054	NN	denotes	β2
T8772	44055-44064	NN	denotes	signaling
T8766	44065-44073	VBD	denotes	appeared
T8773	44074-44076	TO	denotes	to
T8774	44077-44079	VB	denotes	be
T8775	44080-44082	DT	denotes	an
T8777	44083-44092	JJ	denotes	important
T8776	44093-44099	NN	denotes	factor
T8778	44100-44103	IN	denotes	for
T8779	44104-44107	DT	denotes	the
T8781	44108-44113	JJ	denotes	early
T8780	44114-44127	NN	denotes	proliferation
T8782	44128-44132	WDT	denotes	that
T8783	44133-44139	VBZ	denotes	occurs
T8784	44140-44142	IN	denotes	in
T8785	44143-44146	DT	denotes	the
T8787	44147-44157	VBG	denotes	developing
T8788	44158-44162	NN	denotes	hair
T8786	44163-44167	NNS	denotes	buds
T8789	44167-44169	,	denotes	,
T8790	44169-44171	IN	denotes	as
T8791	44172-44178	VBN	denotes	judged
T8792	44179-44181	IN	denotes	by
T8793	44182-44191	JJ	denotes	anti-Ki67
T8795	44192-44195	CC	denotes	and
T8796	44196-44206	JJ	denotes	anti-pMAPK
T8794	44207-44225	NN	denotes	immunofluorescence
T8797	44226-44227	-LRB-	denotes	(
T8799	44227-44233	NN	denotes	Figure
T8798	44234-44236	NN	denotes	6F
T8800	44237-44240	CC	denotes	and
T8801	44241-44243	NN	denotes	6G
T8802	44243-44244	-RRB-	denotes	)
T8803	44244-44245	.	denotes	.
T8804	44245-44417	sentence	denotes	If TGF-β2 stimulates Snail expression in developing buds, loss of this morphogen would be expected to affect the expression of genes that are typically repressed by Snail.
T8805	44246-44248	IN	denotes	If
T8807	44249-44252	NN	denotes	TGF
T8809	44252-44253	HYPH	denotes	-
T8808	44253-44255	NN	denotes	β2
T8806	44256-44266	VBZ	denotes	stimulates
T8811	44267-44272	NN	denotes	Snail
T8812	44273-44283	NN	denotes	expression
T8813	44284-44286	IN	denotes	in
T8814	44287-44297	VBG	denotes	developing
T8815	44298-44302	NNS	denotes	buds
T8816	44302-44304	,	denotes	,
T8817	44304-44308	NN	denotes	loss
T8818	44309-44311	IN	denotes	of
T8819	44312-44316	DT	denotes	this
T8820	44317-44326	NN	denotes	morphogen
T8821	44327-44332	MD	denotes	would
T8822	44333-44335	VB	denotes	be
T8810	44336-44344	VBN	denotes	expected
T8823	44345-44347	TO	denotes	to
T8824	44348-44354	VB	denotes	affect
T8825	44355-44358	DT	denotes	the
T8826	44359-44369	NN	denotes	expression
T8827	44370-44372	IN	denotes	of
T8828	44373-44378	NNS	denotes	genes
T8829	44379-44383	WDT	denotes	that
T8831	44384-44387	VBP	denotes	are
T8832	44388-44397	RB	denotes	typically
T8830	44398-44407	VBN	denotes	repressed
T8833	44408-44410	IN	denotes	by
T8834	44411-44416	NN	denotes	Snail
T8835	44416-44417	.	denotes	.
T8836	44417-44562	sentence	denotes	Since a major target for Snail-mediated repression is the E-cadherin gene [12,13], we investigated the status of E-cadherin in TGF-β2-null buds.
T8837	44418-44423	IN	denotes	Since
T8839	44424-44425	DT	denotes	a
T8841	44426-44431	JJ	denotes	major
T8840	44432-44438	NN	denotes	target
T8842	44439-44442	IN	denotes	for
T8843	44443-44448	NN	denotes	Snail
T8845	44448-44449	HYPH	denotes	-
T8844	44449-44457	VBN	denotes	mediated
T8846	44458-44468	NN	denotes	repression
T8838	44469-44471	VBZ	denotes	is
T8848	44472-44475	DT	denotes	the
T8850	44476-44477	NN	denotes	E
T8852	44477-44478	HYPH	denotes	-
T8851	44478-44486	NN	denotes	cadherin
T8849	44487-44491	NN	denotes	gene
T8853	44492-44493	-LRB-	denotes	[
T8855	44493-44495	CD	denotes	12
T8856	44495-44496	,	denotes	,
T8854	44496-44498	CD	denotes	13
T8857	44498-44499	-RRB-	denotes	]
T8858	44499-44501	,	denotes	,
T8859	44501-44503	PRP	denotes	we
T8847	44504-44516	VBD	denotes	investigated
T8860	44517-44520	DT	denotes	the
T8861	44521-44527	NN	denotes	status
T8862	44528-44530	IN	denotes	of
T8863	44531-44532	NN	denotes	E
T8865	44532-44533	HYPH	denotes	-
T8864	44533-44541	NN	denotes	cadherin
T8866	44542-44544	IN	denotes	in
T8867	44545-44548	NN	denotes	TGF
T8869	44548-44549	HYPH	denotes	-
T8868	44549-44551	NN	denotes	β2
T8871	44551-44552	HYPH	denotes	-
T8870	44552-44556	JJ	denotes	null
T8872	44557-44561	NNS	denotes	buds
T8873	44561-44562	.	denotes	.
T8874	44562-44697	sentence	denotes	As shown in Figure 6H, hair buds in TGF-β2 null skin displayed elevated immunofluorescence staining relative to their WT counterparts.
T8875	44563-44565	IN	denotes	As
T8876	44566-44571	VBN	denotes	shown
T8878	44572-44574	IN	denotes	in
T8879	44575-44581	NN	denotes	Figure
T8880	44582-44584	NN	denotes	6H
T8881	44584-44586	,	denotes	,
T8882	44586-44590	NN	denotes	hair
T8883	44591-44595	NNS	denotes	buds
T8884	44596-44598	IN	denotes	in
T8885	44599-44602	NN	denotes	TGF
T8887	44602-44603	HYPH	denotes	-
T8886	44603-44605	NN	denotes	β2
T8888	44606-44610	JJ	denotes	null
T8889	44611-44615	NN	denotes	skin
T8877	44616-44625	VBD	denotes	displayed
T8890	44626-44634	JJ	denotes	elevated
T8892	44635-44653	NN	denotes	immunofluorescence
T8891	44654-44662	NN	denotes	staining
T8893	44663-44671	JJ	denotes	relative
T8894	44672-44674	IN	denotes	to
T8895	44675-44680	PRP$	denotes	their
T8897	44681-44683	NN	denotes	WT
T8896	44684-44696	NNS	denotes	counterparts
T8898	44696-44697	.	denotes	.
T8899	44697-44946	sentence	denotes	Previously we demonstrated that the concerted action of the extracellular signals Wnt and noggin are required for the generation of a LEF-1/β-catenin transcription complex to repress E-cadherin transcription at the onset of hair fate specification.
T8900	44698-44708	RB	denotes	Previously
T8902	44709-44711	PRP	denotes	we
T8901	44712-44724	VBD	denotes	demonstrated
T8903	44725-44729	IN	denotes	that
T8905	44730-44733	DT	denotes	the
T8907	44734-44743	JJ	denotes	concerted
T8906	44744-44750	NN	denotes	action
T8908	44751-44753	IN	denotes	of
T8909	44754-44757	DT	denotes	the
T8911	44758-44771	JJ	denotes	extracellular
T8910	44772-44779	NNS	denotes	signals
T8912	44780-44783	NN	denotes	Wnt
T8913	44784-44787	CC	denotes	and
T8914	44788-44794	NN	denotes	noggin
T8915	44795-44798	VBP	denotes	are
T8904	44799-44807	VBN	denotes	required
T8916	44808-44811	IN	denotes	for
T8918	44812-44815	DT	denotes	the
T8919	44816-44826	NN	denotes	generation
T8920	44827-44829	IN	denotes	of
T8921	44830-44831	DT	denotes	a
T8923	44832-44835	NN	denotes	LEF
T8924	44835-44836	HYPH	denotes	-
T8925	44836-44837	CD	denotes	1
T8926	44837-44838	HYPH	denotes	/
T8927	44838-44839	NN	denotes	β
T8929	44839-44840	HYPH	denotes	-
T8928	44840-44847	NN	denotes	catenin
T8930	44848-44861	NN	denotes	transcription
T8922	44862-44869	NN	denotes	complex
T8931	44870-44872	TO	denotes	to
T8917	44873-44880	VB	denotes	repress
T8932	44881-44882	NN	denotes	E
T8934	44882-44883	HYPH	denotes	-
T8933	44883-44891	NN	denotes	cadherin
T8935	44892-44905	NN	denotes	transcription
T8936	44906-44908	IN	denotes	at
T8937	44909-44912	DT	denotes	the
T8938	44913-44918	NN	denotes	onset
T8939	44919-44921	IN	denotes	of
T8940	44922-44926	NN	denotes	hair
T8941	44927-44931	NN	denotes	fate
T8942	44932-44945	NN	denotes	specification
T8943	44945-44946	.	denotes	.
T8944	44946-45113	sentence	denotes	As shown in Figure 6I and 6J, both WT and TGF-β2 null buds exhibited nuclear LEF-1 and β-catenin localization, signs that the Wnt-noggin signaling pathway was intact.
T8945	44947-44949	IN	denotes	As
T8946	44950-44955	VBN	denotes	shown
T8948	44956-44958	IN	denotes	in
T8949	44959-44965	NN	denotes	Figure
T8950	44966-44968	NN	denotes	6I
T8951	44969-44972	CC	denotes	and
T8952	44973-44975	NN	denotes	6J
T8953	44975-44977	,	denotes	,
T8954	44977-44981	CC	denotes	both
T8955	44982-44984	NN	denotes	WT
T8957	44985-44988	CC	denotes	and
T8958	44989-44992	NN	denotes	TGF
T8960	44992-44993	HYPH	denotes	-
T8959	44993-44995	NN	denotes	β2
T8956	44996-45000	JJ	denotes	null
T8961	45001-45005	NNS	denotes	buds
T8947	45006-45015	VBD	denotes	exhibited
T8962	45016-45023	JJ	denotes	nuclear
T8964	45024-45027	NN	denotes	LEF
T8965	45027-45028	HYPH	denotes	-
T8966	45028-45029	CD	denotes	1
T8967	45030-45033	CC	denotes	and
T8968	45034-45035	NN	denotes	β
T8970	45035-45036	HYPH	denotes	-
T8969	45036-45043	NN	denotes	catenin
T8963	45044-45056	NN	denotes	localization
T8971	45056-45058	,	denotes	,
T8972	45058-45063	NNS	denotes	signs
T8973	45064-45068	IN	denotes	that
T8975	45069-45072	DT	denotes	the
T8977	45073-45076	NN	denotes	Wnt
T8979	45076-45077	HYPH	denotes	-
T8978	45077-45083	NN	denotes	noggin
T8980	45084-45093	NN	denotes	signaling
T8976	45094-45101	NN	denotes	pathway
T8974	45102-45105	VBD	denotes	was
T8981	45106-45112	JJ	denotes	intact
T8982	45112-45113	.	denotes	.
T8983	45113-45254	sentence	denotes	These data suggest that during hair follicle morphogenesis, TGF-β2 functions subsequently to Wnt/noggin-mediated determination of hair fate.
T8984	45114-45119	DT	denotes	These
T8985	45120-45124	NNS	denotes	data
T8986	45125-45132	VBP	denotes	suggest
T8987	45133-45137	IN	denotes	that
T8989	45138-45144	IN	denotes	during
T8990	45145-45149	NN	denotes	hair
T8991	45150-45158	NN	denotes	follicle
T8992	45159-45172	NN	denotes	morphogenesis
T8993	45172-45174	,	denotes	,
T8994	45174-45177	NN	denotes	TGF
T8996	45177-45178	HYPH	denotes	-
T8995	45178-45180	NN	denotes	β2
T8988	45181-45190	VBZ	denotes	functions
T8997	45191-45203	RB	denotes	subsequently
T8998	45204-45206	IN	denotes	to
T8999	45207-45210	NN	denotes	Wnt
T9001	45210-45211	HYPH	denotes	/
T9000	45211-45217	NN	denotes	noggin
T9003	45217-45218	HYPH	denotes	-
T9002	45218-45226	VBN	denotes	mediated
T9004	45227-45240	NN	denotes	determination
T9005	45241-45243	IN	denotes	of
T9006	45244-45248	NN	denotes	hair
T9007	45249-45253	NN	denotes	fate
T9008	45253-45254	.	denotes	.
T9009	45254-45472	sentence	denotes	Moreover, through activation of Snail gene expression, TGF-β2 appears to work in tandem with these other morphogens to down-regulate E-cadherin levels, which contributes to the activation of proliferative circuitries.
T9010	45255-45263	RB	denotes	Moreover
T9012	45263-45265	,	denotes	,
T9013	45265-45272	IN	denotes	through
T9014	45273-45283	NN	denotes	activation
T9015	45284-45286	IN	denotes	of
T9016	45287-45292	NN	denotes	Snail
T9018	45293-45297	NN	denotes	gene
T9017	45298-45308	NN	denotes	expression
T9019	45308-45310	,	denotes	,
T9020	45310-45313	NN	denotes	TGF
T9022	45313-45314	HYPH	denotes	-
T9021	45314-45316	NN	denotes	β2
T9011	45317-45324	VBZ	denotes	appears
T9023	45325-45327	TO	denotes	to
T9024	45328-45332	VB	denotes	work
T9025	45333-45335	IN	denotes	in
T9026	45336-45342	NN	denotes	tandem
T9027	45343-45347	IN	denotes	with
T9028	45348-45353	DT	denotes	these
T9030	45354-45359	JJ	denotes	other
T9029	45360-45370	NNS	denotes	morphogens
T9031	45371-45373	TO	denotes	to
T9033	45374-45378	RB	denotes	down
T9034	45378-45379	HYPH	denotes	-
T9032	45379-45387	VB	denotes	regulate
T9035	45388-45389	NN	denotes	E
T9037	45389-45390	HYPH	denotes	-
T9036	45390-45398	NN	denotes	cadherin
T9038	45399-45405	NNS	denotes	levels
T9039	45405-45407	,	denotes	,
T9040	45407-45412	WDT	denotes	which
T9041	45413-45424	VBZ	denotes	contributes
T9042	45425-45427	IN	denotes	to
T9043	45428-45431	DT	denotes	the
T9044	45432-45442	NN	denotes	activation
T9045	45443-45445	IN	denotes	of
T9046	45446-45459	JJ	denotes	proliferative
T9047	45460-45471	NNS	denotes	circuitries
T9048	45471-45472	.	denotes	.
T9102	45485-45491	IN	denotes	During
T9104	45492-45499	NN	denotes	budding
T9105	45500-45513	NN	denotes	morphogenesis
T9106	45513-45515	,	denotes	,
T9107	45515-45527	VBG	denotes	intersecting
T9109	45528-45537	NN	denotes	signaling
T9108	45538-45546	NNS	denotes	networks
T9110	45547-45551	IN	denotes	from
T9111	45552-45555	DT	denotes	the
T9112	45556-45566	NN	denotes	epithelium
T9113	45567-45570	CC	denotes	and
T9114	45571-45581	NN	denotes	mesenchyme
T9103	45582-45588	VBP	denotes	govern
T9115	45589-45604	JJ	denotes	transcriptional
T9117	45604-45606	,	denotes	,
T9118	45606-45614	JJ	denotes	adhesive
T9119	45614-45616	,	denotes	,
T9120	45616-45624	NN	denotes	polarity
T9121	45624-45626	,	denotes	,
T9122	45626-45629	CC	denotes	and
T9123	45630-45638	NN	denotes	motility
T9116	45639-45647	NNS	denotes	programs
T9124	45648-45650	IN	denotes	in
T9125	45651-45656	DT	denotes	these
T9127	45657-45663	JJ	denotes	select
T9126	45664-45670	NNS	denotes	groups
T9128	45671-45673	IN	denotes	of
T9129	45674-45679	NNS	denotes	cells
T9130	45679-45680	.	denotes	.
T9131	45680-45801	sentence	denotes	The dynamic nuclear and cytosolic changes that take place during this time form the cornerstone for organ morphogenesis.
T9132	45681-45684	DT	denotes	The
T9134	45685-45692	JJ	denotes	dynamic
T9135	45693-45700	JJ	denotes	nuclear
T9136	45701-45704	CC	denotes	and
T9137	45705-45714	JJ	denotes	cytosolic
T9133	45715-45722	NNS	denotes	changes
T9139	45723-45727	WDT	denotes	that
T9140	45728-45732	VBP	denotes	take
T9141	45733-45738	NN	denotes	place
T9142	45739-45745	IN	denotes	during
T9143	45746-45750	DT	denotes	this
T9144	45751-45755	NN	denotes	time
T9138	45756-45760	VBP	denotes	form
T9145	45761-45764	DT	denotes	the
T9146	45765-45776	NN	denotes	cornerstone
T9147	45777-45780	IN	denotes	for
T9148	45781-45786	NN	denotes	organ
T9149	45787-45800	NN	denotes	morphogenesis
T9150	45800-45801	.	denotes	.
T9151	45801-46122	sentence	denotes	Two major challenges in understanding the mechanisms underlying a particular budding process are to order the temporal sequence of external cues involved and then to dissect how the cells of the developing bud translate these signals into the downstream events of cellular remodeling, proliferation, and differentiation.
T9152	45802-45805	CD	denotes	Two
T9154	45806-45811	JJ	denotes	major
T9153	45812-45822	NNS	denotes	challenges
T9156	45823-45825	IN	denotes	in
T9157	45826-45839	VBG	denotes	understanding
T9158	45840-45843	DT	denotes	the
T9159	45844-45854	NNS	denotes	mechanisms
T9160	45855-45865	VBG	denotes	underlying
T9161	45866-45867	DT	denotes	a
T9163	45868-45878	JJ	denotes	particular
T9164	45879-45886	NN	denotes	budding
T9162	45887-45894	NN	denotes	process
T9155	45895-45898	VBP	denotes	are
T9165	45899-45901	TO	denotes	to
T9166	45902-45907	VB	denotes	order
T9167	45908-45911	DT	denotes	the
T9169	45912-45920	JJ	denotes	temporal
T9168	45921-45929	NN	denotes	sequence
T9170	45930-45932	IN	denotes	of
T9171	45933-45941	JJ	denotes	external
T9172	45942-45946	NNS	denotes	cues
T9173	45947-45955	VBN	denotes	involved
T9174	45956-45959	CC	denotes	and
T9175	45960-45964	RB	denotes	then
T9177	45965-45967	TO	denotes	to
T9176	45968-45975	VB	denotes	dissect
T9178	45976-45979	WRB	denotes	how
T9180	45980-45983	DT	denotes	the
T9181	45984-45989	NNS	denotes	cells
T9182	45990-45992	IN	denotes	of
T9183	45993-45996	DT	denotes	the
T9185	45997-46007	VBG	denotes	developing
T9184	46008-46011	NN	denotes	bud
T9179	46012-46021	VB	denotes	translate
T9186	46022-46027	DT	denotes	these
T9187	46028-46035	NNS	denotes	signals
T9188	46036-46040	IN	denotes	into
T9189	46041-46044	DT	denotes	the
T9191	46045-46055	JJ	denotes	downstream
T9190	46056-46062	NNS	denotes	events
T9192	46063-46065	IN	denotes	of
T9193	46066-46074	JJ	denotes	cellular
T9194	46075-46085	NN	denotes	remodeling
T9195	46085-46087	,	denotes	,
T9196	46087-46100	NN	denotes	proliferation
T9197	46100-46102	,	denotes	,
T9198	46102-46105	CC	denotes	and
T9199	46106-46121	NN	denotes	differentiation
T9200	46121-46122	.	denotes	.
T9201	46122-46246	sentence	denotes	Our studies here provide some insights into how these events are orchestrated during hair bud formation in developing skin.
T9202	46123-46126	PRP$	denotes	Our
T9203	46127-46134	NNS	denotes	studies
T9205	46135-46139	RB	denotes	here
T9204	46140-46147	VBP	denotes	provide
T9206	46148-46152	DT	denotes	some
T9207	46153-46161	NNS	denotes	insights
T9208	46162-46166	IN	denotes	into
T9209	46167-46170	WRB	denotes	how
T9211	46171-46176	DT	denotes	these
T9212	46177-46183	NNS	denotes	events
T9213	46184-46187	VBP	denotes	are
T9210	46188-46200	VBN	denotes	orchestrated
T9214	46201-46207	IN	denotes	during
T9215	46208-46212	NN	denotes	hair
T9216	46213-46216	NN	denotes	bud
T9217	46217-46226	NN	denotes	formation
T9218	46227-46229	IN	denotes	in
T9219	46230-46240	VBG	denotes	developing
T9220	46241-46245	NN	denotes	skin
T9221	46245-46246	.	denotes	.
T9653	46248-46257	NN	denotes	Signaling
T9654	46258-46264	IN	denotes	during
T9655	46265-46270	JJ	denotes	Early
T9657	46271-46275	NN	denotes	Hair
T9658	46276-46284	NN	denotes	Follicle
T9656	46285-46298	NN	denotes	Morphogenesis
T9659	46298-46624	sentence	denotes	Recent studies on hair bud morphogenesis suggest that Wnt signals likely from the epithelium and BMP inhibitory signals from the underlying mesenchyme converge to produce an active transcription factor complex involving β-catenin and LEF-1, which in turn plays a key role in specifying the hair follicle fate [4,29,30,36,37].
T9660	46299-46305	JJ	denotes	Recent
T9661	46306-46313	NNS	denotes	studies
T9663	46314-46316	IN	denotes	on
T9664	46317-46321	NN	denotes	hair
T9665	46322-46325	NN	denotes	bud
T9666	46326-46339	NN	denotes	morphogenesis
T9662	46340-46347	VBP	denotes	suggest
T9667	46348-46352	IN	denotes	that
T9669	46353-46356	NN	denotes	Wnt
T9670	46357-46364	NNS	denotes	signals
T9671	46365-46371	RB	denotes	likely
T9672	46372-46376	IN	denotes	from
T9673	46377-46380	DT	denotes	the
T9674	46381-46391	NN	denotes	epithelium
T9675	46392-46395	CC	denotes	and
T9676	46396-46399	NN	denotes	BMP
T9678	46400-46410	JJ	denotes	inhibitory
T9677	46411-46418	NNS	denotes	signals
T9679	46419-46423	IN	denotes	from
T9680	46424-46427	DT	denotes	the
T9682	46428-46438	VBG	denotes	underlying
T9681	46439-46449	NN	denotes	mesenchyme
T9668	46450-46458	VBP	denotes	converge
T9683	46459-46461	TO	denotes	to
T9684	46462-46469	VB	denotes	produce
T9685	46470-46472	DT	denotes	an
T9687	46473-46479	JJ	denotes	active
T9688	46480-46493	NN	denotes	transcription
T9689	46494-46500	NN	denotes	factor
T9686	46501-46508	NN	denotes	complex
T9690	46509-46518	VBG	denotes	involving
T9691	46519-46520	NN	denotes	β
T9693	46520-46521	HYPH	denotes	-
T9692	46521-46528	NN	denotes	catenin
T9694	46529-46532	CC	denotes	and
T9695	46533-46536	NN	denotes	LEF
T9696	46536-46537	HYPH	denotes	-
T9697	46537-46538	CD	denotes	1
T9698	46538-46540	,	denotes	,
T9699	46540-46545	WDT	denotes	which
T9701	46546-46548	IN	denotes	in
T9702	46549-46553	NN	denotes	turn
T9700	46554-46559	VBZ	denotes	plays
T9703	46560-46561	DT	denotes	a
T9705	46562-46565	JJ	denotes	key
T9704	46566-46570	NN	denotes	role
T9706	46571-46573	IN	denotes	in
T9707	46574-46584	VBG	denotes	specifying
T9708	46585-46588	DT	denotes	the
T9710	46589-46593	NN	denotes	hair
T9711	46594-46602	NN	denotes	follicle
T9709	46603-46607	NN	denotes	fate
T9712	46608-46609	-LRB-	denotes	[
T9714	46609-46610	CD	denotes	4
T9715	46610-46611	,	denotes	,
T9716	46611-46613	CD	denotes	29
T9717	46613-46614	,	denotes	,
T9718	46614-46616	CD	denotes	30
T9719	46616-46617	,	denotes	,
T9720	46617-46619	CD	denotes	36
T9721	46619-46620	,	denotes	,
T9713	46620-46622	CD	denotes	37
T9722	46622-46623	-RRB-	denotes	]
T9723	46623-46624	.	denotes	.
T9724	46624-46883	sentence	denotes	Sonic hedgehog (Shh) and TGF-β2 signaling also play essential roles in follicle morphogenesis, but in contrast to β-catenin null skin, in which follicle invaginations are absent [30], some hair buds still form in the absence of LEF-1, Shh, or TGF-β2 [32,38].
T9725	46625-46630	JJ	denotes	Sonic
T9726	46631-46639	NN	denotes	hedgehog
T9728	46640-46641	-LRB-	denotes	(
T9729	46641-46644	NN	denotes	Shh
T9730	46644-46645	-RRB-	denotes	)
T9731	46646-46649	CC	denotes	and
T9732	46650-46653	NN	denotes	TGF
T9734	46653-46654	HYPH	denotes	-
T9733	46654-46656	NN	denotes	β2
T9735	46657-46666	NN	denotes	signaling
T9736	46667-46671	RB	denotes	also
T9727	46672-46676	VBP	denotes	play
T9737	46677-46686	JJ	denotes	essential
T9738	46687-46692	NNS	denotes	roles
T9739	46693-46695	IN	denotes	in
T9740	46696-46704	NN	denotes	follicle
T9741	46705-46718	NN	denotes	morphogenesis
T9742	46718-46720	,	denotes	,
T9743	46720-46723	CC	denotes	but
T9744	46724-46726	IN	denotes	in
T9746	46727-46735	NN	denotes	contrast
T9747	46736-46738	IN	denotes	to
T9748	46739-46740	NN	denotes	β
T9750	46740-46741	HYPH	denotes	-
T9749	46741-46748	NN	denotes	catenin
T9751	46749-46753	JJ	denotes	null
T9752	46754-46758	NN	denotes	skin
T9753	46758-46760	,	denotes	,
T9754	46760-46762	IN	denotes	in
T9756	46763-46768	WDT	denotes	which
T9757	46769-46777	NN	denotes	follicle
T9758	46778-46791	NNS	denotes	invaginations
T9755	46792-46795	VBP	denotes	are
T9759	46796-46802	JJ	denotes	absent
T9760	46803-46804	-LRB-	denotes	[
T9761	46804-46806	CD	denotes	30
T9762	46806-46807	-RRB-	denotes	]
T9763	46807-46809	,	denotes	,
T9764	46809-46813	DT	denotes	some
T9766	46814-46818	NN	denotes	hair
T9765	46819-46823	NNS	denotes	buds
T9767	46824-46829	RB	denotes	still
T9745	46830-46834	VBP	denotes	form
T9768	46835-46837	IN	denotes	in
T9769	46838-46841	DT	denotes	the
T9770	46842-46849	NN	denotes	absence
T9771	46850-46852	IN	denotes	of
T9772	46853-46856	NN	denotes	LEF
T9773	46856-46857	HYPH	denotes	-
T9774	46857-46858	CD	denotes	1
T9775	46858-46860	,	denotes	,
T9776	46860-46863	NN	denotes	Shh
T9777	46863-46865	,	denotes	,
T9778	46865-46867	CC	denotes	or
T9779	46868-46871	NN	denotes	TGF
T9781	46871-46872	HYPH	denotes	-
T9780	46872-46874	NN	denotes	β2
T9782	46875-46876	-LRB-	denotes	[
T9784	46876-46878	CD	denotes	32
T9785	46878-46879	,	denotes	,
T9783	46879-46881	CD	denotes	38
T9786	46881-46882	-RRB-	denotes	]
T9787	46882-46883	.	denotes	.
T9788	46883-47066	sentence	denotes	These likely reflect the first wave of follicle (i.e., guard hair) morphogenesis, which accounts for a small number (fewer than 5%) of hairs and is under distinct regulatory control.
T9789	46884-46889	DT	denotes	These
T9791	46890-46896	RB	denotes	likely
T9790	46897-46904	VBP	denotes	reflect
T9792	46905-46908	DT	denotes	the
T9794	46909-46914	JJ	denotes	first
T9793	46915-46919	NN	denotes	wave
T9795	46920-46922	IN	denotes	of
T9796	46923-46931	NN	denotes	follicle
T9798	46932-46933	-LRB-	denotes	(
T9800	46933-46937	FW	denotes	i.e.
T9801	46937-46939	,	denotes	,
T9802	46939-46944	NN	denotes	guard
T9799	46945-46949	NN	denotes	hair
T9803	46949-46950	-RRB-	denotes	)
T9797	46951-46964	NN	denotes	morphogenesis
T9804	46964-46966	,	denotes	,
T9805	46966-46971	WDT	denotes	which
T9806	46972-46980	VBZ	denotes	accounts
T9807	46981-46984	IN	denotes	for
T9808	46985-46986	DT	denotes	a
T9810	46987-46992	JJ	denotes	small
T9809	46993-46999	NN	denotes	number
T9811	47000-47001	-LRB-	denotes	(
T9812	47001-47006	JJR	denotes	fewer
T9814	47007-47011	IN	denotes	than
T9813	47012-47013	CD	denotes	5
T9815	47013-47014	NN	denotes	%
T9816	47014-47015	-RRB-	denotes	)
T9817	47016-47018	IN	denotes	of
T9818	47019-47024	NNS	denotes	hairs
T9819	47025-47028	CC	denotes	and
T9820	47029-47031	VBZ	denotes	is
T9821	47032-47037	IN	denotes	under
T9822	47038-47046	JJ	denotes	distinct
T9824	47047-47057	JJ	denotes	regulatory
T9823	47058-47065	NN	denotes	control
T9825	47065-47066	.	denotes	.
T9826	47066-47230	sentence	denotes	Guard hairs form in the absence of LEF-1 and TGF-β2, and we have found that they also fail to express Snail at the budding stage of development (unpublished data).
T9827	47067-47072	NN	denotes	Guard
T9828	47073-47078	NNS	denotes	hairs
T9829	47079-47083	VBP	denotes	form
T9830	47084-47086	IN	denotes	in
T9831	47087-47090	DT	denotes	the
T9832	47091-47098	NN	denotes	absence
T9833	47099-47101	IN	denotes	of
T9834	47102-47105	NN	denotes	LEF
T9835	47105-47106	HYPH	denotes	-
T9836	47106-47107	CD	denotes	1
T9837	47108-47111	CC	denotes	and
T9838	47112-47115	NN	denotes	TGF
T9840	47115-47116	HYPH	denotes	-
T9839	47116-47118	NN	denotes	β2
T9841	47118-47120	,	denotes	,
T9842	47120-47123	CC	denotes	and
T9843	47124-47126	PRP	denotes	we
T9845	47127-47131	VBP	denotes	have
T9844	47132-47137	VBN	denotes	found
T9846	47138-47142	IN	denotes	that
T9848	47143-47147	PRP	denotes	they
T9849	47148-47152	RB	denotes	also
T9847	47153-47157	VBP	denotes	fail
T9850	47158-47160	TO	denotes	to
T9851	47161-47168	VB	denotes	express
T9852	47169-47174	NN	denotes	Snail
T9853	47175-47177	IN	denotes	at
T9854	47178-47181	DT	denotes	the
T9856	47182-47189	NN	denotes	budding
T9855	47190-47195	NN	denotes	stage
T9857	47196-47198	IN	denotes	of
T9858	47199-47210	NN	denotes	development
T9859	47211-47212	-LRB-	denotes	(
T9861	47212-47223	JJ	denotes	unpublished
T9860	47224-47228	NNS	denotes	data
T9862	47228-47229	-RRB-	denotes	)
T9863	47229-47230	.	denotes	.
T9864	47230-47299	sentence	denotes	How E-cadherin is regulated in guard hairs remains to be determined.
T9865	47231-47234	WRB	denotes	How
T9867	47235-47236	NN	denotes	E
T9869	47236-47237	HYPH	denotes	-
T9868	47237-47245	NN	denotes	cadherin
T9870	47246-47248	VBZ	denotes	is
T9866	47249-47258	VBN	denotes	regulated
T9872	47259-47261	IN	denotes	in
T9873	47262-47267	NN	denotes	guard
T9874	47268-47273	NNS	denotes	hairs
T9871	47274-47281	VBZ	denotes	remains
T9875	47282-47284	TO	denotes	to
T9877	47285-47287	VB	denotes	be
T9876	47288-47298	VBN	denotes	determined
T9878	47298-47299	.	denotes	.
T9879	47299-47533	sentence	denotes	Several candidates include other Snail family members such as Slug or twist, or alternatively, transcription factors involving β-catenin and a different member of the LEF-1/TCF/Sry-type HMG box (commonly known as SOX) family [39,40].
T9880	47300-47307	JJ	denotes	Several
T9881	47308-47318	NNS	denotes	candidates
T9882	47319-47326	VBP	denotes	include
T9883	47327-47332	JJ	denotes	other
T9885	47333-47338	NN	denotes	Snail
T9886	47339-47345	NN	denotes	family
T9884	47346-47353	NNS	denotes	members
T9887	47354-47358	JJ	denotes	such
T9888	47359-47361	IN	denotes	as
T9889	47362-47366	NN	denotes	Slug
T9890	47367-47369	CC	denotes	or
T9891	47370-47375	NN	denotes	twist
T9892	47375-47377	,	denotes	,
T9893	47377-47379	CC	denotes	or
T9894	47380-47393	RB	denotes	alternatively
T9896	47393-47395	,	denotes	,
T9897	47395-47408	NN	denotes	transcription
T9895	47409-47416	NNS	denotes	factors
T9898	47417-47426	VBG	denotes	involving
T9899	47427-47428	NN	denotes	β
T9901	47428-47429	HYPH	denotes	-
T9900	47429-47436	NN	denotes	catenin
T9902	47437-47440	CC	denotes	and
T9903	47441-47442	DT	denotes	a
T9905	47443-47452	JJ	denotes	different
T9904	47453-47459	NN	denotes	member
T9906	47460-47462	IN	denotes	of
T9907	47463-47466	DT	denotes	the
T9909	47467-47470	NN	denotes	LEF
T9911	47470-47471	HYPH	denotes	-
T9912	47471-47472	CD	denotes	1
T9913	47472-47473	HYPH	denotes	/
T9914	47473-47476	NN	denotes	TCF
T9915	47476-47477	HYPH	denotes	/
T9916	47477-47480	NN	denotes	Sry
T9918	47480-47481	HYPH	denotes	-
T9917	47481-47485	NN	denotes	type
T9919	47486-47489	NN	denotes	HMG
T9910	47490-47493	NN	denotes	box
T9920	47494-47495	-LRB-	denotes	(
T9922	47495-47503	RB	denotes	commonly
T9921	47504-47509	VBN	denotes	known
T9923	47510-47512	IN	denotes	as
T9924	47513-47516	NN	denotes	SOX
T9925	47516-47517	-RRB-	denotes	)
T9908	47518-47524	NN	denotes	family
T9926	47525-47526	-LRB-	denotes	[
T9928	47526-47528	CD	denotes	39
T9929	47528-47529	,	denotes	,
T9927	47529-47531	CD	denotes	40
T9930	47531-47532	-RRB-	denotes	]
T9931	47532-47533	.	denotes	.
T9932	47533-47676	sentence	denotes	Further investigation will be required to determine whether the signaling pathway we have elucidated here is a theme with multiple variations.
T9933	47534-47541	JJ	denotes	Further
T9934	47542-47555	NN	denotes	investigation
T9936	47556-47560	MD	denotes	will
T9937	47561-47563	VB	denotes	be
T9935	47564-47572	VBN	denotes	required
T9938	47573-47575	TO	denotes	to
T9939	47576-47585	VB	denotes	determine
T9940	47586-47593	IN	denotes	whether
T9942	47594-47597	DT	denotes	the
T9944	47598-47607	NN	denotes	signaling
T9943	47608-47615	NN	denotes	pathway
T9945	47616-47618	PRP	denotes	we
T9947	47619-47623	VBP	denotes	have
T9946	47624-47634	VBN	denotes	elucidated
T9948	47635-47639	RB	denotes	here
T9941	47640-47642	VBZ	denotes	is
T9949	47643-47644	DT	denotes	a
T9950	47645-47650	NN	denotes	theme
T9951	47651-47655	IN	denotes	with
T9952	47656-47664	JJ	denotes	multiple
T9953	47665-47675	NNS	denotes	variations
T9954	47675-47676	.	denotes	.
T9955	47676-47758	sentence	denotes	TGF-βs are known to promote withdrawal of keratinocytes from the cell cycle [41].
T9956	47677-47680	NN	denotes	TGF
T9958	47680-47681	HYPH	denotes	-
T9957	47681-47683	NNS	denotes	βs
T9960	47684-47687	VBP	denotes	are
T9959	47688-47693	VBN	denotes	known
T9961	47694-47696	TO	denotes	to
T9962	47697-47704	VB	denotes	promote
T9963	47705-47715	NN	denotes	withdrawal
T9964	47716-47718	IN	denotes	of
T9965	47719-47732	NNS	denotes	keratinocytes
T9966	47733-47737	IN	denotes	from
T9967	47738-47741	DT	denotes	the
T9969	47742-47746	NN	denotes	cell
T9968	47747-47752	NN	denotes	cycle
T9970	47753-47754	-LRB-	denotes	[
T9971	47754-47756	CD	denotes	41
T9972	47756-47757	-RRB-	denotes	]
T9973	47757-47758	.	denotes	.
T9974	47758-48040	sentence	denotes	Hence, when TGF-β2 protein was detected at the transition between the growing and destructive phases of the adult hair cycle, research initially and naturally focused on a role for this family member in cessation of growth and/or triggering apoptosis ([42] and references therein).
T9975	47759-47764	RB	denotes	Hence
T9977	47764-47766	,	denotes	,
T9978	47766-47770	WRB	denotes	when
T9980	47771-47774	NN	denotes	TGF
T9982	47774-47775	HYPH	denotes	-
T9981	47775-47777	NN	denotes	β2
T9983	47778-47785	NN	denotes	protein
T9984	47786-47789	VBD	denotes	was
T9979	47790-47798	VBN	denotes	detected
T9985	47799-47801	IN	denotes	at
T9986	47802-47805	DT	denotes	the
T9987	47806-47816	NN	denotes	transition
T9988	47817-47824	IN	denotes	between
T9989	47825-47828	DT	denotes	the
T9991	47829-47836	NN	denotes	growing
T9993	47837-47840	CC	denotes	and
T9992	47841-47852	JJ	denotes	destructive
T9990	47853-47859	NNS	denotes	phases
T9994	47860-47862	IN	denotes	of
T9995	47863-47866	DT	denotes	the
T9997	47867-47872	JJ	denotes	adult
T9998	47873-47877	NN	denotes	hair
T9996	47878-47883	NN	denotes	cycle
T9999	47883-47885	,	denotes	,
T10000	47885-47893	NN	denotes	research
T10001	47894-47903	RB	denotes	initially
T10002	47904-47907	CC	denotes	and
T10003	47908-47917	RB	denotes	naturally
T9976	47918-47925	VBD	denotes	focused
T10004	47926-47928	IN	denotes	on
T10005	47929-47930	DT	denotes	a
T10006	47931-47935	NN	denotes	role
T10007	47936-47939	IN	denotes	for
T10008	47940-47944	DT	denotes	this
T10010	47945-47951	NN	denotes	family
T10009	47952-47958	NN	denotes	member
T10011	47959-47961	IN	denotes	in
T10012	47962-47971	NN	denotes	cessation
T10013	47972-47974	IN	denotes	of
T10014	47975-47981	NN	denotes	growth
T10015	47982-47985	CC	denotes	and
T10016	47985-47986	HYPH	denotes	/
T10017	47986-47988	CC	denotes	or
T10018	47989-47999	VBG	denotes	triggering
T10019	48000-48009	NN	denotes	apoptosis
T10020	48010-48011	-LRB-	denotes	(
T10022	48011-48012	-LRB-	denotes	[
T10021	48012-48014	CD	denotes	42
T10023	48014-48015	-RRB-	denotes	]
T10024	48016-48019	CC	denotes	and
T10025	48020-48030	NNS	denotes	references
T10026	48031-48038	RB	denotes	therein
T10027	48038-48039	-RRB-	denotes	)
T10028	48039-48040	.	denotes	.
T10029	48040-48231	sentence	denotes	However, in contrast to TGF-β1-null skin, which exhibits an extended growing phase of postnatal hair follicles, TGF-β2-null skin displays an embryonic block in follicle bud progression [32].
T10030	48041-48048	RB	denotes	However
T10032	48048-48050	,	denotes	,
T10033	48050-48052	IN	denotes	in
T10034	48053-48061	NN	denotes	contrast
T10035	48062-48064	IN	denotes	to
T10036	48065-48068	NN	denotes	TGF
T10038	48068-48069	HYPH	denotes	-
T10037	48069-48071	NN	denotes	β1
T10040	48071-48072	HYPH	denotes	-
T10039	48072-48076	JJ	denotes	null
T10041	48077-48081	NN	denotes	skin
T10042	48081-48083	,	denotes	,
T10043	48083-48088	WDT	denotes	which
T10044	48089-48097	VBZ	denotes	exhibits
T10045	48098-48100	DT	denotes	an
T10047	48101-48109	JJ	denotes	extended
T10048	48110-48117	NN	denotes	growing
T10046	48118-48123	NN	denotes	phase
T10049	48124-48126	IN	denotes	of
T10050	48127-48136	JJ	denotes	postnatal
T10052	48137-48141	NN	denotes	hair
T10051	48142-48151	NNS	denotes	follicles
T10053	48151-48153	,	denotes	,
T10054	48153-48156	NN	denotes	TGF
T10056	48156-48157	HYPH	denotes	-
T10055	48157-48159	NN	denotes	β2
T10058	48159-48160	HYPH	denotes	-
T10057	48160-48164	JJ	denotes	null
T10059	48165-48169	NN	denotes	skin
T10031	48170-48178	VBZ	denotes	displays
T10060	48179-48181	DT	denotes	an
T10062	48182-48191	JJ	denotes	embryonic
T10061	48192-48197	NN	denotes	block
T10063	48198-48200	IN	denotes	in
T10064	48201-48209	NN	denotes	follicle
T10065	48210-48213	NN	denotes	bud
T10066	48214-48225	NN	denotes	progression
T10067	48226-48227	-LRB-	denotes	[
T10068	48227-48229	CD	denotes	32
T10069	48229-48230	-RRB-	denotes	]
T10070	48230-48231	.	denotes	.
T10071	48231-48502	sentence	denotes	Although this phenotype is consistent with TGF-β2's embryonic expression patterns [33], about 50% of TGF-β2 null buds appear unable to progress to the down-growth phase, a feature that cannot be explained readily on the basis of previously established effects of TGF-βs.
T10072	48232-48240	IN	denotes	Although
T10074	48241-48245	DT	denotes	this
T10075	48246-48255	NN	denotes	phenotype
T10073	48256-48258	VBZ	denotes	is
T10077	48259-48269	JJ	denotes	consistent
T10078	48270-48274	IN	denotes	with
T10079	48275-48278	NN	denotes	TGF
T10081	48278-48279	HYPH	denotes	-
T10080	48279-48281	NN	denotes	β2
T10083	48281-48283	POS	denotes	's
T10084	48284-48293	JJ	denotes	embryonic
T10085	48294-48304	NN	denotes	expression
T10082	48305-48313	NNS	denotes	patterns
T10086	48314-48315	-LRB-	denotes	[
T10087	48315-48317	CD	denotes	33
T10088	48317-48318	-RRB-	denotes	]
T10089	48318-48320	,	denotes	,
T10090	48320-48325	IN	denotes	about
T10091	48326-48328	CD	denotes	50
T10092	48328-48329	NN	denotes	%
T10093	48330-48332	IN	denotes	of
T10094	48333-48336	NN	denotes	TGF
T10096	48336-48337	HYPH	denotes	-
T10095	48337-48339	NN	denotes	β2
T10097	48340-48344	JJ	denotes	null
T10098	48345-48349	NNS	denotes	buds
T10076	48350-48356	VBP	denotes	appear
T10099	48357-48363	JJ	denotes	unable
T10100	48364-48366	TO	denotes	to
T10101	48367-48375	VB	denotes	progress
T10102	48376-48378	IN	denotes	to
T10103	48379-48382	DT	denotes	the
T10105	48383-48387	JJ	denotes	down
T10107	48387-48388	HYPH	denotes	-
T10106	48388-48394	NN	denotes	growth
T10104	48395-48400	NN	denotes	phase
T10108	48400-48402	,	denotes	,
T10109	48402-48403	DT	denotes	a
T10110	48404-48411	NN	denotes	feature
T10111	48412-48416	WDT	denotes	that
T10113	48417-48420	MD	denotes	can
T10114	48420-48423	RB	denotes	not
T10115	48424-48426	VB	denotes	be
T10112	48427-48436	VBN	denotes	explained
T10116	48437-48444	RB	denotes	readily
T10117	48445-48447	IN	denotes	on
T10118	48448-48451	DT	denotes	the
T10119	48452-48457	NN	denotes	basis
T10120	48458-48460	IN	denotes	of
T10121	48461-48471	RB	denotes	previously
T10122	48472-48483	VBN	denotes	established
T10123	48484-48491	NNS	denotes	effects
T10124	48492-48494	IN	denotes	of
T10125	48495-48498	NN	denotes	TGF
T10127	48498-48499	HYPH	denotes	-
T10126	48499-48501	NNS	denotes	βs
T10128	48501-48502	.	denotes	.
T10129	48502-48777	sentence	denotes	Our finding that TGF-β2 is upstream from Ki67 expression and MAPK activation lends further support to the notion that hair follicle keratinocytes at this early stage of development react to TGF-β2 signaling in a fashion opposite to that typically expected for TGF-β factors.
T10130	48503-48506	PRP$	denotes	Our
T10131	48507-48514	NN	denotes	finding
T10133	48515-48519	IN	denotes	that
T10135	48520-48523	NN	denotes	TGF
T10137	48523-48524	HYPH	denotes	-
T10136	48524-48526	NN	denotes	β2
T10134	48527-48529	VBZ	denotes	is
T10138	48530-48538	RB	denotes	upstream
T10139	48539-48543	IN	denotes	from
T10140	48544-48548	NN	denotes	Ki67
T10141	48549-48559	NN	denotes	expression
T10142	48560-48563	CC	denotes	and
T10143	48564-48568	NN	denotes	MAPK
T10144	48569-48579	NN	denotes	activation
T10132	48580-48585	VBZ	denotes	lends
T10145	48586-48593	JJ	denotes	further
T10146	48594-48601	NN	denotes	support
T10147	48602-48604	IN	denotes	to
T10148	48605-48608	DT	denotes	the
T10149	48609-48615	NN	denotes	notion
T10150	48616-48620	IN	denotes	that
T10152	48621-48625	NN	denotes	hair
T10153	48626-48634	NN	denotes	follicle
T10154	48635-48648	NNS	denotes	keratinocytes
T10155	48649-48651	IN	denotes	at
T10156	48652-48656	DT	denotes	this
T10158	48657-48662	JJ	denotes	early
T10157	48663-48668	NN	denotes	stage
T10159	48669-48671	IN	denotes	of
T10160	48672-48683	NN	denotes	development
T10151	48684-48689	VBP	denotes	react
T10161	48690-48692	IN	denotes	to
T10162	48693-48696	NN	denotes	TGF
T10164	48696-48697	HYPH	denotes	-
T10163	48697-48699	NN	denotes	β2
T10165	48700-48709	NN	denotes	signaling
T10166	48710-48712	IN	denotes	in
T10167	48713-48714	DT	denotes	a
T10168	48715-48722	NN	denotes	fashion
T10169	48723-48731	JJ	denotes	opposite
T10170	48732-48734	IN	denotes	to
T10171	48735-48739	DT	denotes	that
T10172	48740-48749	RB	denotes	typically
T10173	48750-48758	VBN	denotes	expected
T10174	48759-48762	IN	denotes	for
T10175	48763-48766	NN	denotes	TGF
T10177	48766-48767	HYPH	denotes	-
T10176	48767-48768	NN	denotes	β
T10178	48769-48776	NNS	denotes	factors
T10179	48776-48777	.	denotes	.
T10180	48777-48895	sentence	denotes	This said, based upon pSMAD2 immunohistochemistry, the immediate steps of downstream signaling appeared to be intact.
T10181	48778-48782	DT	denotes	This
T10182	48783-48787	VBN	denotes	said
T10184	48787-48789	,	denotes	,
T10185	48789-48794	VBN	denotes	based
T10186	48795-48799	IN	denotes	upon
T10187	48800-48806	NN	denotes	pSMAD2
T10188	48807-48827	NN	denotes	immunohistochemistry
T10189	48827-48829	,	denotes	,
T10190	48829-48832	DT	denotes	the
T10192	48833-48842	JJ	denotes	immediate
T10191	48843-48848	NNS	denotes	steps
T10193	48849-48851	IN	denotes	of
T10194	48852-48862	JJ	denotes	downstream
T10195	48863-48872	NN	denotes	signaling
T10183	48873-48881	VBD	denotes	appeared
T10196	48882-48884	TO	denotes	to
T10197	48885-48887	VB	denotes	be
T10198	48888-48894	JJ	denotes	intact
T10199	48894-48895	.	denotes	.
T10200	48895-49031	sentence	denotes	Thus, we surmise that the proliferative outcome is likely to be rooted in differences in the repertoire of activated SMAD target genes.
T10201	48896-48900	RB	denotes	Thus
T10203	48900-48902	,	denotes	,
T10204	48902-48904	PRP	denotes	we
T10202	48905-48912	VBP	denotes	surmise
T10205	48913-48917	IN	denotes	that
T10207	48918-48921	DT	denotes	the
T10209	48922-48935	JJ	denotes	proliferative
T10208	48936-48943	NN	denotes	outcome
T10206	48944-48946	VBZ	denotes	is
T10210	48947-48953	JJ	denotes	likely
T10211	48954-48956	TO	denotes	to
T10213	48957-48959	VB	denotes	be
T10212	48960-48966	VBN	denotes	rooted
T10214	48967-48969	IN	denotes	in
T10215	48970-48981	NNS	denotes	differences
T10216	48982-48984	IN	denotes	in
T10217	48985-48988	DT	denotes	the
T10218	48989-48999	NN	denotes	repertoire
T10219	49000-49002	IN	denotes	of
T10220	49003-49012	VBN	denotes	activated
T10222	49013-49017	NN	denotes	SMAD
T10223	49018-49024	NN	denotes	target
T10221	49025-49030	NNS	denotes	genes
T10224	49030-49031	.	denotes	.
T10225	49031-49296	sentence	denotes	In this regard, the positive effects of TGF-β2 on proliferation within the hair bud may be more analogous to what has been seen in progression of squamous cell carcinoma to metastatic carcinoma [43] rather than that typically observed for keratinocytes [44,45,46].
T10226	49032-49034	IN	denotes	In
T10228	49035-49039	DT	denotes	this
T10229	49040-49046	NN	denotes	regard
T10230	49046-49048	,	denotes	,
T10231	49048-49051	DT	denotes	the
T10233	49052-49060	JJ	denotes	positive
T10232	49061-49068	NNS	denotes	effects
T10234	49069-49071	IN	denotes	of
T10235	49072-49075	NN	denotes	TGF
T10237	49075-49076	HYPH	denotes	-
T10236	49076-49078	NN	denotes	β2
T10238	49079-49081	IN	denotes	on
T10239	49082-49095	NN	denotes	proliferation
T10240	49096-49102	IN	denotes	within
T10241	49103-49106	DT	denotes	the
T10243	49107-49111	NN	denotes	hair
T10242	49112-49115	NN	denotes	bud
T10244	49116-49119	MD	denotes	may
T10227	49120-49122	VB	denotes	be
T10245	49123-49127	RBR	denotes	more
T10246	49128-49137	JJ	denotes	analogous
T10247	49138-49140	IN	denotes	to
T10248	49141-49145	WP	denotes	what
T10250	49146-49149	VBZ	denotes	has
T10251	49150-49154	VBN	denotes	been
T10249	49155-49159	VBN	denotes	seen
T10252	49160-49162	IN	denotes	in
T10253	49163-49174	NN	denotes	progression
T10254	49175-49177	IN	denotes	of
T10255	49178-49186	JJ	denotes	squamous
T10256	49187-49191	NN	denotes	cell
T10257	49192-49201	NN	denotes	carcinoma
T10258	49202-49204	IN	denotes	to
T10259	49205-49215	JJ	denotes	metastatic
T10260	49216-49225	NN	denotes	carcinoma
T10261	49226-49227	-LRB-	denotes	[
T10262	49227-49229	CD	denotes	43
T10263	49229-49230	-RRB-	denotes	]
T10264	49231-49237	RB	denotes	rather
T10265	49238-49242	IN	denotes	than
T10266	49243-49247	DT	denotes	that
T10267	49248-49257	RB	denotes	typically
T10268	49258-49266	VBN	denotes	observed
T10269	49267-49270	IN	denotes	for
T10270	49271-49284	NNS	denotes	keratinocytes
T10271	49285-49286	-LRB-	denotes	[
T10273	49286-49288	CD	denotes	44
T10274	49288-49289	,	denotes	,
T10275	49289-49291	CD	denotes	45
T10276	49291-49292	,	denotes	,
T10272	49292-49294	CD	denotes	46
T10277	49294-49295	-RRB-	denotes	]
T10278	49295-49296	.	denotes	.
T10279	49296-49490	sentence	denotes	The prior identification of the Snail gene as a potential target of TGF-β signaling [15] was intriguing, given the temporal wave of Snail gene expression that occurs in the developing hair bud.
T10280	49297-49300	DT	denotes	The
T10282	49301-49306	JJ	denotes	prior
T10281	49307-49321	NN	denotes	identification
T10284	49322-49324	IN	denotes	of
T10285	49325-49328	DT	denotes	the
T10287	49329-49334	NN	denotes	Snail
T10286	49335-49339	NN	denotes	gene
T10288	49340-49342	IN	denotes	as
T10289	49343-49344	DT	denotes	a
T10291	49345-49354	JJ	denotes	potential
T10290	49355-49361	NN	denotes	target
T10292	49362-49364	IN	denotes	of
T10293	49365-49368	NN	denotes	TGF
T10295	49368-49369	HYPH	denotes	-
T10294	49369-49370	NN	denotes	β
T10296	49371-49380	NN	denotes	signaling
T10297	49381-49382	-LRB-	denotes	[
T10298	49382-49384	CD	denotes	15
T10299	49384-49385	-RRB-	denotes	]
T10283	49386-49389	VBD	denotes	was
T10300	49390-49400	JJ	denotes	intriguing
T10301	49400-49402	,	denotes	,
T10302	49402-49407	VBN	denotes	given
T10303	49408-49411	DT	denotes	the
T10305	49412-49420	JJ	denotes	temporal
T10304	49421-49425	NN	denotes	wave
T10306	49426-49428	IN	denotes	of
T10307	49429-49434	NN	denotes	Snail
T10309	49435-49439	NN	denotes	gene
T10308	49440-49450	NN	denotes	expression
T10310	49451-49455	WDT	denotes	that
T10311	49456-49462	VBZ	denotes	occurs
T10312	49463-49465	IN	denotes	in
T10313	49466-49469	DT	denotes	the
T10315	49470-49480	VBG	denotes	developing
T10316	49481-49485	NN	denotes	hair
T10314	49486-49489	NN	denotes	bud
T10317	49489-49490	.	denotes	.
T10318	49490-49661	sentence	denotes	The additional correlation between epithelial hyperproliferation and Snail transgene expression further fostered our interest in a possible link between TGF-β2 and Snail.
T10319	49491-49494	DT	denotes	The
T10321	49495-49505	JJ	denotes	additional
T10320	49506-49517	NN	denotes	correlation
T10323	49518-49525	IN	denotes	between
T10324	49526-49536	JJ	denotes	epithelial
T10325	49537-49555	NN	denotes	hyperproliferation
T10326	49556-49559	CC	denotes	and
T10327	49560-49565	NN	denotes	Snail
T10329	49566-49575	NN	denotes	transgene
T10328	49576-49586	NN	denotes	expression
T10330	49587-49594	RB	denotes	further
T10322	49595-49603	VBD	denotes	fostered
T10331	49604-49607	PRP$	denotes	our
T10332	49608-49616	NN	denotes	interest
T10333	49617-49619	IN	denotes	in
T10334	49620-49621	DT	denotes	a
T10336	49622-49630	JJ	denotes	possible
T10335	49631-49635	NN	denotes	link
T10337	49636-49643	IN	denotes	between
T10338	49644-49647	NN	denotes	TGF
T10340	49647-49648	HYPH	denotes	-
T10339	49648-49650	NN	denotes	β2
T10341	49651-49654	CC	denotes	and
T10342	49655-49660	NN	denotes	Snail
T10343	49660-49661	.	denotes	.
T10344	49661-49839	sentence	denotes	Our functional studies demonstrate that without TGF-β2, Snail expression is abolished in the mutant hair buds, and conversely, in K14-Smad2 skin, Snail is ectopically activated.
T10345	49662-49665	PRP$	denotes	Our
T10347	49666-49676	JJ	denotes	functional
T10346	49677-49684	NNS	denotes	studies
T10348	49685-49696	VBP	denotes	demonstrate
T10349	49697-49701	IN	denotes	that
T10351	49702-49709	IN	denotes	without
T10352	49710-49713	NN	denotes	TGF
T10354	49713-49714	HYPH	denotes	-
T10353	49714-49716	NN	denotes	β2
T10355	49716-49718	,	denotes	,
T10356	49718-49723	NN	denotes	Snail
T10357	49724-49734	NN	denotes	expression
T10358	49735-49737	VBZ	denotes	is
T10350	49738-49747	VBN	denotes	abolished
T10359	49748-49750	IN	denotes	in
T10360	49751-49754	DT	denotes	the
T10362	49755-49761	NN	denotes	mutant
T10363	49762-49766	NN	denotes	hair
T10361	49767-49771	NNS	denotes	buds
T10364	49771-49773	,	denotes	,
T10365	49773-49776	CC	denotes	and
T10366	49777-49787	RB	denotes	conversely
T10368	49787-49789	,	denotes	,
T10369	49789-49791	IN	denotes	in
T10370	49792-49795	NN	denotes	K14
T10372	49795-49796	HYPH	denotes	-
T10371	49796-49801	NN	denotes	Smad2
T10373	49802-49806	NN	denotes	skin
T10374	49806-49808	,	denotes	,
T10375	49808-49813	NN	denotes	Snail
T10376	49814-49816	VBZ	denotes	is
T10377	49817-49828	RB	denotes	ectopically
T10367	49829-49838	VBN	denotes	activated
T10378	49838-49839	.	denotes	.
T10379	49839-50070	sentence	denotes	Moreover, our in vitro studies indicate that even sustained TGF-β2 exposure may cause only a transient induction of Snail, offering a possible explanation as to why Snail is so briefly expressed during hair follicle morphogenesis.
T10380	49840-49848	RB	denotes	Moreover
T10382	49848-49850	,	denotes	,
T10383	49850-49853	PRP$	denotes	our
T10385	49854-49856	FW	denotes	in
T10386	49857-49862	FW	denotes	vitro
T10384	49863-49870	NNS	denotes	studies
T10381	49871-49879	VBP	denotes	indicate
T10387	49880-49884	IN	denotes	that
T10389	49885-49889	RB	denotes	even
T10391	49890-49899	JJ	denotes	sustained
T10392	49900-49903	NN	denotes	TGF
T10394	49903-49904	HYPH	denotes	-
T10393	49904-49906	NN	denotes	β2
T10390	49907-49915	NN	denotes	exposure
T10395	49916-49919	MD	denotes	may
T10388	49920-49925	VB	denotes	cause
T10396	49926-49930	RB	denotes	only
T10398	49931-49932	DT	denotes	a
T10399	49933-49942	JJ	denotes	transient
T10397	49943-49952	NN	denotes	induction
T10400	49953-49955	IN	denotes	of
T10401	49956-49961	NN	denotes	Snail
T10402	49961-49963	,	denotes	,
T10403	49963-49971	VBG	denotes	offering
T10404	49972-49973	DT	denotes	a
T10406	49974-49982	JJ	denotes	possible
T10405	49983-49994	NN	denotes	explanation
T10407	49995-49997	IN	denotes	as
T10408	49998-50000	IN	denotes	to
T10409	50001-50004	WRB	denotes	why
T10411	50005-50010	NN	denotes	Snail
T10412	50011-50013	VBZ	denotes	is
T10413	50014-50016	RB	denotes	so
T10414	50017-50024	RB	denotes	briefly
T10410	50025-50034	VBN	denotes	expressed
T10415	50035-50041	IN	denotes	during
T10416	50042-50046	NN	denotes	hair
T10417	50047-50055	NN	denotes	follicle
T10418	50056-50069	NN	denotes	morphogenesis
T10419	50069-50070	.	denotes	.
T10420	50070-50387	sentence	denotes	An additional point worth mentioning is that prolonged expression of Tg Snail in postnatal skin resulted in morphological and biochemical signs of epithelial to mesenchymal transitions (unpublished data), underscoring why transient Snail expression may be so important during normal hair follicle morphogenesis [18].
T10421	50071-50073	DT	denotes	An
T10423	50074-50084	JJ	denotes	additional
T10422	50085-50090	NN	denotes	point
T10425	50091-50096	JJ	denotes	worth
T10426	50097-50107	VBG	denotes	mentioning
T10424	50108-50110	VBZ	denotes	is
T10427	50111-50115	IN	denotes	that
T10429	50116-50125	VBN	denotes	prolonged
T10430	50126-50136	NN	denotes	expression
T10431	50137-50139	IN	denotes	of
T10432	50140-50142	NN	denotes	Tg
T10433	50143-50148	NN	denotes	Snail
T10434	50149-50151	IN	denotes	in
T10435	50152-50161	JJ	denotes	postnatal
T10436	50162-50166	NN	denotes	skin
T10428	50167-50175	VBD	denotes	resulted
T10437	50176-50178	IN	denotes	in
T10438	50179-50192	JJ	denotes	morphological
T10440	50193-50196	CC	denotes	and
T10441	50197-50208	JJ	denotes	biochemical
T10439	50209-50214	NNS	denotes	signs
T10442	50215-50217	IN	denotes	of
T10443	50218-50228	JJ	denotes	epithelial
T10445	50229-50231	IN	denotes	to
T10446	50232-50243	JJ	denotes	mesenchymal
T10444	50244-50255	NNS	denotes	transitions
T10447	50256-50257	-LRB-	denotes	(
T10449	50257-50268	JJ	denotes	unpublished
T10448	50269-50273	NNS	denotes	data
T10450	50273-50274	-RRB-	denotes	)
T10451	50274-50276	,	denotes	,
T10452	50276-50288	VBG	denotes	underscoring
T10453	50289-50292	WRB	denotes	why
T10455	50293-50302	JJ	denotes	transient
T10457	50303-50308	NN	denotes	Snail
T10456	50309-50319	NN	denotes	expression
T10458	50320-50323	MD	denotes	may
T10454	50324-50326	VB	denotes	be
T10459	50327-50329	RB	denotes	so
T10460	50330-50339	JJ	denotes	important
T10461	50340-50346	IN	denotes	during
T10462	50347-50353	JJ	denotes	normal
T10464	50354-50358	NN	denotes	hair
T10465	50359-50367	NN	denotes	follicle
T10463	50368-50381	NN	denotes	morphogenesis
T10466	50382-50383	-LRB-	denotes	[
T10467	50383-50385	CD	denotes	18
T10468	50385-50386	-RRB-	denotes	]
T10469	50386-50387	.	denotes	.
T10470	50387-50520	sentence	denotes	At first glance, the sparsity in hair coat of K14-Snail Tg mice seemed indicative of a defect in follicle formation (see Figure 2A).
T10471	50388-50390	IN	denotes	At
T10473	50391-50396	JJ	denotes	first
T10474	50397-50403	NN	denotes	glance
T10475	50403-50405	,	denotes	,
T10476	50405-50408	DT	denotes	the
T10477	50409-50417	NN	denotes	sparsity
T10478	50418-50420	IN	denotes	in
T10479	50421-50425	NN	denotes	hair
T10480	50426-50430	NN	denotes	coat
T10481	50431-50433	IN	denotes	of
T10482	50434-50437	NN	denotes	K14
T10484	50437-50438	HYPH	denotes	-
T10483	50438-50443	NN	denotes	Snail
T10486	50444-50446	NN	denotes	Tg
T10485	50447-50451	NNS	denotes	mice
T10472	50452-50458	VBD	denotes	seemed
T10487	50459-50469	JJ	denotes	indicative
T10488	50470-50472	IN	denotes	of
T10489	50473-50474	DT	denotes	a
T10490	50475-50481	NN	denotes	defect
T10491	50482-50484	IN	denotes	in
T10492	50485-50493	NN	denotes	follicle
T10493	50494-50503	NN	denotes	formation
T10494	50504-50505	-LRB-	denotes	(
T10495	50505-50508	VB	denotes	see
T10496	50509-50515	NN	denotes	Figure
T10497	50516-50518	NN	denotes	2A
T10498	50518-50519	-RRB-	denotes	)
T10499	50519-50520	.	denotes	.
T10500	50520-50620	sentence	denotes	Closer inspection, however, revealed that not all hairs penetrated the hyperthickened Tg epidermis.
T10501	50521-50527	JJR	denotes	Closer
T10502	50528-50538	NN	denotes	inspection
T10504	50538-50540	,	denotes	,
T10505	50540-50547	RB	denotes	however
T10506	50547-50549	,	denotes	,
T10503	50549-50557	VBD	denotes	revealed
T10507	50558-50562	IN	denotes	that
T10509	50563-50566	RB	denotes	not
T10511	50567-50570	DT	denotes	all
T10510	50571-50576	NNS	denotes	hairs
T10508	50577-50587	VBD	denotes	penetrated
T10512	50588-50591	DT	denotes	the
T10514	50592-50606	VBN	denotes	hyperthickened
T10515	50607-50609	NN	denotes	Tg
T10513	50610-50619	NN	denotes	epidermis
T10516	50619-50620	.	denotes	.
T10517	50620-50711	sentence	denotes	Several factors may contribute to the seemingly normal follicle development in these mice.
T10518	50621-50628	JJ	denotes	Several
T10519	50629-50636	NNS	denotes	factors
T10521	50637-50640	MD	denotes	may
T10520	50641-50651	VB	denotes	contribute
T10522	50652-50654	IN	denotes	to
T10523	50655-50658	DT	denotes	the
T10525	50659-50668	RB	denotes	seemingly
T10526	50669-50675	JJ	denotes	normal
T10527	50676-50684	NN	denotes	follicle
T10524	50685-50696	NN	denotes	development
T10528	50697-50699	IN	denotes	in
T10529	50700-50705	DT	denotes	these
T10530	50706-50710	NNS	denotes	mice
T10531	50710-50711	.	denotes	.
T10532	50711-50975	sentence	denotes	One obvious factor is the K14 promoter, which is elevated in the basal layer of the epidermis and the outer root sheath (ORS) of the hair follicle, but is markedly down-regulated in developing embryonic hair buds as well as in the postnatal hair progenitor cells.
T10533	50712-50715	CD	denotes	One
T10535	50716-50723	JJ	denotes	obvious
T10534	50724-50730	NN	denotes	factor
T10536	50731-50733	VBZ	denotes	is
T10537	50734-50737	DT	denotes	the
T10539	50738-50741	NN	denotes	K14
T10538	50742-50750	NN	denotes	promoter
T10540	50750-50752	,	denotes	,
T10541	50752-50757	WDT	denotes	which
T10542	50758-50760	VBZ	denotes	is
T10543	50761-50769	JJ	denotes	elevated
T10544	50770-50772	IN	denotes	in
T10545	50773-50776	DT	denotes	the
T10547	50777-50782	JJ	denotes	basal
T10546	50783-50788	NN	denotes	layer
T10548	50789-50791	IN	denotes	of
T10549	50792-50795	DT	denotes	the
T10550	50796-50805	NN	denotes	epidermis
T10551	50806-50809	CC	denotes	and
T10552	50810-50813	DT	denotes	the
T10554	50814-50819	JJ	denotes	outer
T10555	50820-50824	NN	denotes	root
T10553	50825-50831	NN	denotes	sheath
T10556	50832-50833	-LRB-	denotes	(
T10557	50833-50836	NN	denotes	ORS
T10558	50836-50837	-RRB-	denotes	)
T10559	50838-50840	IN	denotes	of
T10560	50841-50844	DT	denotes	the
T10562	50845-50849	NN	denotes	hair
T10561	50850-50858	NN	denotes	follicle
T10563	50858-50860	,	denotes	,
T10564	50860-50863	CC	denotes	but
T10565	50864-50866	VBZ	denotes	is
T10567	50867-50875	RB	denotes	markedly
T10568	50876-50880	RB	denotes	down
T10569	50880-50881	HYPH	denotes	-
T10566	50881-50890	VBN	denotes	regulated
T10570	50891-50893	IN	denotes	in
T10571	50894-50904	JJ	denotes	developing
T10573	50905-50914	JJ	denotes	embryonic
T10574	50915-50919	NN	denotes	hair
T10572	50920-50924	NNS	denotes	buds
T10575	50925-50927	RB	denotes	as
T10577	50928-50932	RB	denotes	well
T10576	50933-50935	IN	denotes	as
T10578	50936-50938	IN	denotes	in
T10579	50939-50942	DT	denotes	the
T10581	50943-50952	JJ	denotes	postnatal
T10582	50953-50957	NN	denotes	hair
T10583	50958-50968	NN	denotes	progenitor
T10580	50969-50974	NNS	denotes	cells
T10584	50974-50975	.	denotes	.
T10585	50975-51135	sentence	denotes	The K14 promoter is also less active in the ORS than epidermis and hence this might also account for the lack of apparent response of the ORS to ectopic Snail.
T10586	50976-50979	DT	denotes	The
T10588	50980-50983	NN	denotes	K14
T10587	50984-50992	NN	denotes	promoter
T10589	50993-50995	VBZ	denotes	is
T10590	50996-51000	RB	denotes	also
T10591	51001-51005	RBR	denotes	less
T10592	51006-51012	JJ	denotes	active
T10593	51013-51015	IN	denotes	in
T10594	51016-51019	DT	denotes	the
T10595	51020-51023	NN	denotes	ORS
T10596	51024-51028	IN	denotes	than
T10597	51029-51038	NN	denotes	epidermis
T10598	51039-51042	CC	denotes	and
T10599	51043-51048	RB	denotes	hence
T10601	51049-51053	DT	denotes	this
T10602	51054-51059	MD	denotes	might
T10603	51060-51064	RB	denotes	also
T10600	51065-51072	VB	denotes	account
T10604	51073-51076	IN	denotes	for
T10605	51077-51080	DT	denotes	the
T10606	51081-51085	NN	denotes	lack
T10607	51086-51088	IN	denotes	of
T10608	51089-51097	JJ	denotes	apparent
T10609	51098-51106	NN	denotes	response
T10610	51107-51109	IN	denotes	of
T10611	51110-51113	DT	denotes	the
T10612	51114-51117	NN	denotes	ORS
T10613	51118-51120	IN	denotes	to
T10614	51121-51128	JJ	denotes	ectopic
T10615	51129-51134	NN	denotes	Snail
T10616	51134-51135	.	denotes	.
T10617	51135-51386	sentence	denotes	Additional contributing factors could be the multiplicity of Snail family members and their differential expression, the saturation, and/or diversity of regulatory mechanisms that govern AJ formation, migration, and proliferation in the follicle ORS.
T10618	51136-51146	JJ	denotes	Additional
T10620	51147-51159	VBG	denotes	contributing
T10619	51160-51167	NNS	denotes	factors
T10622	51168-51173	MD	denotes	could
T10621	51174-51176	VB	denotes	be
T10623	51177-51180	DT	denotes	the
T10624	51181-51193	NN	denotes	multiplicity
T10625	51194-51196	IN	denotes	of
T10626	51197-51202	NN	denotes	Snail
T10628	51203-51209	NN	denotes	family
T10627	51210-51217	NNS	denotes	members
T10629	51218-51221	CC	denotes	and
T10630	51222-51227	PRP$	denotes	their
T10632	51228-51240	JJ	denotes	differential
T10631	51241-51251	NN	denotes	expression
T10633	51251-51253	,	denotes	,
T10634	51253-51256	DT	denotes	the
T10635	51257-51267	NN	denotes	saturation
T10636	51267-51269	,	denotes	,
T10637	51269-51272	CC	denotes	and
T10638	51272-51273	HYPH	denotes	/
T10639	51273-51275	CC	denotes	or
T10640	51276-51285	NN	denotes	diversity
T10641	51286-51288	IN	denotes	of
T10642	51289-51299	JJ	denotes	regulatory
T10643	51300-51310	NNS	denotes	mechanisms
T10644	51311-51315	WDT	denotes	that
T10645	51316-51322	VBP	denotes	govern
T10646	51323-51325	NN	denotes	AJ
T10647	51326-51335	NN	denotes	formation
T10648	51335-51337	,	denotes	,
T10649	51337-51346	NN	denotes	migration
T10650	51346-51348	,	denotes	,
T10651	51348-51351	CC	denotes	and
T10652	51352-51365	NN	denotes	proliferation
T10653	51366-51368	IN	denotes	in
T10654	51369-51372	DT	denotes	the
T10656	51373-51381	NN	denotes	follicle
T10655	51382-51385	NN	denotes	ORS
T10657	51385-51386	.	denotes	.
T10658	51386-51521	sentence	denotes	Distinguishing between these possibilities must await the generation of mice harboring skin-specific loss-of-function Snail mutations.
T10659	51387-51401	VBG	denotes	Distinguishing
T10661	51402-51409	IN	denotes	between
T10662	51410-51415	DT	denotes	these
T10663	51416-51429	NNS	denotes	possibilities
T10664	51430-51434	MD	denotes	must
T10660	51435-51440	VB	denotes	await
T10665	51441-51444	DT	denotes	the
T10666	51445-51455	NN	denotes	generation
T10667	51456-51458	IN	denotes	of
T10668	51459-51463	NNS	denotes	mice
T10669	51464-51473	VBG	denotes	harboring
T10670	51474-51478	NN	denotes	skin
T10672	51478-51479	HYPH	denotes	-
T10671	51479-51487	JJ	denotes	specific
T10674	51488-51492	NN	denotes	loss
T10675	51492-51493	HYPH	denotes	-
T10676	51493-51495	IN	denotes	of
T10677	51495-51496	HYPH	denotes	-
T10678	51496-51504	NN	denotes	function
T10679	51505-51510	NN	denotes	Snail
T10673	51511-51520	NNS	denotes	mutations
T10680	51520-51521	.	denotes	.
T11057	51523-51528	NNS	denotes	Links
T11058	51529-51536	IN	denotes	between
T11059	51537-51546	NN	denotes	Signaling
T11060	51546-51548	,	denotes	,
T11061	51548-51563	JJ	denotes	Transcriptional
T11062	51564-51572	NNS	denotes	Cascades
T11063	51572-51574	,	denotes	,
T11064	51574-51584	JJ	denotes	Epithelial
T11065	51585-51595	NN	denotes	Remodeling
T11066	51595-51597	,	denotes	,
T11067	51597-51600	CC	denotes	and
T11068	51601-51614	NN	denotes	Proliferation
T11069	51615-51617	IN	denotes	in
T11070	51618-51621	DT	denotes	the
T11072	51622-51626	NN	denotes	Hair
T11071	51627-51630	NN	denotes	Bud
T11073	51630-51827	sentence	denotes	Previously, we discovered that early during hair follicle morphogenesis, E-cadherin gene expression is down-regulated concomitantly with the invagination of developing bud cells into the skin [4].
T11074	51631-51641	RB	denotes	Previously
T11076	51641-51643	,	denotes	,
T11077	51643-51645	PRP	denotes	we
T11075	51646-51656	VBD	denotes	discovered
T11078	51657-51661	IN	denotes	that
T11080	51662-51667	RB	denotes	early
T11081	51668-51674	IN	denotes	during
T11082	51675-51679	NN	denotes	hair
T11083	51680-51688	NN	denotes	follicle
T11084	51689-51702	NN	denotes	morphogenesis
T11085	51702-51704	,	denotes	,
T11086	51704-51705	NN	denotes	E
T11088	51705-51706	HYPH	denotes	-
T11087	51706-51714	NN	denotes	cadherin
T11090	51715-51719	NN	denotes	gene
T11089	51720-51730	NN	denotes	expression
T11091	51731-51733	VBZ	denotes	is
T11092	51734-51738	RB	denotes	down
T11093	51738-51739	HYPH	denotes	-
T11079	51739-51748	VBN	denotes	regulated
T11094	51749-51762	RB	denotes	concomitantly
T11095	51763-51767	IN	denotes	with
T11096	51768-51771	DT	denotes	the
T11097	51772-51784	NN	denotes	invagination
T11098	51785-51787	IN	denotes	of
T11099	51788-51798	JJ	denotes	developing
T11101	51799-51802	NN	denotes	bud
T11100	51803-51808	NNS	denotes	cells
T11102	51809-51813	IN	denotes	into
T11103	51814-51817	DT	denotes	the
T11104	51818-51822	NN	denotes	skin
T11105	51823-51824	-LRB-	denotes	[
T11106	51824-51825	CD	denotes	4
T11107	51825-51826	-RRB-	denotes	]
T11108	51826-51827	.	denotes	.
T11109	51827-52046	sentence	denotes	Because the timing of this event correlated with the activation of a LEF-1/β-catenin transcription factor complex [20], we were intrigued by the presence of a putative LEF-1/TCF binding site in the E-cadherin promoter.
T11110	51828-51835	IN	denotes	Because
T11112	51836-51839	DT	denotes	the
T11113	51840-51846	NN	denotes	timing
T11114	51847-51849	IN	denotes	of
T11115	51850-51854	DT	denotes	this
T11116	51855-51860	NN	denotes	event
T11111	51861-51871	VBD	denotes	correlated
T11118	51872-51876	IN	denotes	with
T11119	51877-51880	DT	denotes	the
T11120	51881-51891	NN	denotes	activation
T11121	51892-51894	IN	denotes	of
T11122	51895-51896	DT	denotes	a
T11124	51897-51900	NN	denotes	LEF
T11126	51900-51901	HYPH	denotes	-
T11127	51901-51902	CD	denotes	1
T11128	51902-51903	HYPH	denotes	/
T11129	51903-51904	NN	denotes	β
T11131	51904-51905	HYPH	denotes	-
T11130	51905-51912	NN	denotes	catenin
T11132	51913-51926	NN	denotes	transcription
T11125	51927-51933	NN	denotes	factor
T11123	51934-51941	NN	denotes	complex
T11133	51942-51943	-LRB-	denotes	[
T11134	51943-51945	CD	denotes	20
T11135	51945-51946	-RRB-	denotes	]
T11136	51946-51948	,	denotes	,
T11137	51948-51950	PRP	denotes	we
T11138	51951-51955	VBD	denotes	were
T11117	51956-51965	VBN	denotes	intrigued
T11139	51966-51968	IN	denotes	by
T11140	51969-51972	DT	denotes	the
T11141	51973-51981	NN	denotes	presence
T11142	51982-51984	IN	denotes	of
T11143	51985-51986	DT	denotes	a
T11145	51987-51995	JJ	denotes	putative
T11146	51996-51999	NN	denotes	LEF
T11147	51999-52000	HYPH	denotes	-
T11148	52000-52001	CD	denotes	1
T11149	52001-52002	HYPH	denotes	/
T11150	52002-52005	NN	denotes	TCF
T11151	52006-52013	NN	denotes	binding
T11144	52014-52018	NN	denotes	site
T11152	52019-52021	IN	denotes	in
T11153	52022-52025	DT	denotes	the
T11155	52026-52027	NN	denotes	E
T11157	52027-52028	HYPH	denotes	-
T11156	52028-52036	NN	denotes	cadherin
T11154	52037-52045	NN	denotes	promoter
T11158	52045-52046	.	denotes	.
T11159	52046-52223	sentence	denotes	This prompted an investigation that subsequently led to our discovery that LEF-1/β-catenin can contribute to repression of E-cadherin gene expression in skin keratinocytes [4].
T11160	52047-52051	DT	denotes	This
T11161	52052-52060	VBD	denotes	prompted
T11162	52061-52063	DT	denotes	an
T11163	52064-52077	NN	denotes	investigation
T11164	52078-52082	WDT	denotes	that
T11166	52083-52095	RB	denotes	subsequently
T11165	52096-52099	VBD	denotes	led
T11167	52100-52102	IN	denotes	to
T11168	52103-52106	PRP$	denotes	our
T11169	52107-52116	NN	denotes	discovery
T11170	52117-52121	IN	denotes	that
T11172	52122-52125	NN	denotes	LEF
T11173	52125-52126	HYPH	denotes	-
T11174	52126-52127	CD	denotes	1
T11175	52127-52128	HYPH	denotes	/
T11176	52128-52129	NN	denotes	β
T11178	52129-52130	HYPH	denotes	-
T11177	52130-52137	NN	denotes	catenin
T11179	52138-52141	MD	denotes	can
T11171	52142-52152	VB	denotes	contribute
T11180	52153-52155	IN	denotes	to
T11181	52156-52166	NN	denotes	repression
T11182	52167-52169	IN	denotes	of
T11183	52170-52171	NN	denotes	E
T11185	52171-52172	HYPH	denotes	-
T11184	52172-52180	NN	denotes	cadherin
T11187	52181-52185	NN	denotes	gene
T11186	52186-52196	NN	denotes	expression
T11188	52197-52199	IN	denotes	in
T11189	52200-52204	NN	denotes	skin
T11190	52205-52218	NNS	denotes	keratinocytes
T11191	52219-52220	-LRB-	denotes	[
T11192	52220-52221	CD	denotes	4
T11193	52221-52222	-RRB-	denotes	]
T11194	52222-52223	.	denotes	.
T11195	52223-52475	sentence	denotes	In the course of these studies, we also noted that Snail can also contribute to this process in keratinocytes in vitro, and our present studies revealed that Snail is expressed at the right place and time to be physiologically relevant in the process.
T11196	52224-52226	IN	denotes	In
T11198	52227-52230	DT	denotes	the
T11199	52231-52237	NN	denotes	course
T11200	52238-52240	IN	denotes	of
T11201	52241-52246	DT	denotes	these
T11202	52247-52254	NNS	denotes	studies
T11203	52254-52256	,	denotes	,
T11204	52256-52258	PRP	denotes	we
T11205	52259-52263	RB	denotes	also
T11197	52264-52269	VBD	denotes	noted
T11206	52270-52274	IN	denotes	that
T11208	52275-52280	NN	denotes	Snail
T11209	52281-52284	MD	denotes	can
T11210	52285-52289	RB	denotes	also
T11207	52290-52300	VB	denotes	contribute
T11211	52301-52303	IN	denotes	to
T11212	52304-52308	DT	denotes	this
T11213	52309-52316	NN	denotes	process
T11214	52317-52319	IN	denotes	in
T11215	52320-52333	NNS	denotes	keratinocytes
T11216	52334-52336	FW	denotes	in
T11217	52337-52342	FW	denotes	vitro
T11218	52342-52344	,	denotes	,
T11219	52344-52347	CC	denotes	and
T11220	52348-52351	PRP$	denotes	our
T11222	52352-52359	JJ	denotes	present
T11221	52360-52367	NNS	denotes	studies
T11223	52368-52376	VBD	denotes	revealed
T11224	52377-52381	IN	denotes	that
T11226	52382-52387	NN	denotes	Snail
T11227	52388-52390	VBZ	denotes	is
T11225	52391-52400	VBN	denotes	expressed
T11228	52401-52403	IN	denotes	at
T11229	52404-52407	DT	denotes	the
T11231	52408-52413	JJ	denotes	right
T11230	52414-52419	NN	denotes	place
T11232	52420-52423	CC	denotes	and
T11233	52424-52428	NN	denotes	time
T11234	52429-52431	TO	denotes	to
T11235	52432-52434	VB	denotes	be
T11236	52435-52450	RB	denotes	physiologically
T11237	52451-52459	JJ	denotes	relevant
T11238	52460-52462	IN	denotes	in
T11239	52463-52466	DT	denotes	the
T11240	52467-52474	NN	denotes	process
T11241	52474-52475	.	denotes	.
T11242	52475-52627	sentence	denotes	In noggin-null embryonic skin, LEF-1 expression and subsequent activation of the LEF-1/β-catenin reporter gene is abrogated in the developing placodes.
T11243	52476-52478	IN	denotes	In
T11245	52479-52485	NN	denotes	noggin
T11247	52485-52486	HYPH	denotes	-
T11246	52486-52490	JJ	denotes	null
T11249	52491-52500	JJ	denotes	embryonic
T11248	52501-52505	NN	denotes	skin
T11250	52505-52507	,	denotes	,
T11251	52507-52510	NN	denotes	LEF
T11253	52510-52511	HYPH	denotes	-
T11254	52511-52512	CD	denotes	1
T11252	52513-52523	NN	denotes	expression
T11255	52524-52527	CC	denotes	and
T11256	52528-52538	JJ	denotes	subsequent
T11257	52539-52549	NN	denotes	activation
T11258	52550-52552	IN	denotes	of
T11259	52553-52556	DT	denotes	the
T11261	52557-52560	NN	denotes	LEF
T11262	52560-52561	HYPH	denotes	-
T11263	52561-52562	CD	denotes	1
T11264	52562-52563	HYPH	denotes	/
T11265	52563-52564	NN	denotes	β
T11267	52564-52565	HYPH	denotes	-
T11266	52565-52572	NN	denotes	catenin
T11268	52573-52581	NN	denotes	reporter
T11260	52582-52586	NN	denotes	gene
T11269	52587-52589	VBZ	denotes	is
T11244	52590-52599	VBN	denotes	abrogated
T11270	52600-52602	IN	denotes	in
T11271	52603-52606	DT	denotes	the
T11273	52607-52617	VBG	denotes	developing
T11272	52618-52626	NNS	denotes	placodes
T11274	52626-52627	.	denotes	.
T11275	52627-52790	sentence	denotes	The corresponding failure of E-cadherin down-regulation underscores the importance of Wnt/noggin signaling in regulating this event in follicle morphogenesis [4].
T11276	52628-52631	DT	denotes	The
T11278	52632-52645	VBG	denotes	corresponding
T11277	52646-52653	NN	denotes	failure
T11280	52654-52656	IN	denotes	of
T11281	52657-52658	NN	denotes	E
T11283	52658-52659	HYPH	denotes	-
T11282	52659-52667	NN	denotes	cadherin
T11285	52668-52672	JJ	denotes	down
T11286	52672-52673	HYPH	denotes	-
T11284	52673-52683	NN	denotes	regulation
T11279	52684-52695	VBZ	denotes	underscores
T11287	52696-52699	DT	denotes	the
T11288	52700-52710	NN	denotes	importance
T11289	52711-52713	IN	denotes	of
T11290	52714-52717	NN	denotes	Wnt
T11292	52717-52718	HYPH	denotes	/
T11291	52718-52724	NN	denotes	noggin
T11293	52725-52734	NN	denotes	signaling
T11294	52735-52737	IN	denotes	in
T11295	52738-52748	VBG	denotes	regulating
T11296	52749-52753	DT	denotes	this
T11297	52754-52759	NN	denotes	event
T11298	52760-52762	IN	denotes	in
T11299	52763-52771	NN	denotes	follicle
T11300	52772-52785	NN	denotes	morphogenesis
T11301	52786-52787	-LRB-	denotes	[
T11302	52787-52788	CD	denotes	4
T11303	52788-52789	-RRB-	denotes	]
T11304	52789-52790	.	denotes	.
T11305	52790-52993	sentence	denotes	Conditional gene targeting studies will be necessary to establish whether Snail family members also contribute to the down-regulation in E-cadherin gene expression that occurs during follicle formation.
T11306	52791-52802	JJ	denotes	Conditional
T11308	52803-52807	NN	denotes	gene
T11309	52808-52817	NN	denotes	targeting
T11307	52818-52825	NNS	denotes	studies
T11311	52826-52830	MD	denotes	will
T11310	52831-52833	VB	denotes	be
T11312	52834-52843	JJ	denotes	necessary
T11313	52844-52846	TO	denotes	to
T11314	52847-52856	VB	denotes	establish
T11315	52857-52864	IN	denotes	whether
T11317	52865-52870	NN	denotes	Snail
T11319	52871-52877	NN	denotes	family
T11318	52878-52885	NNS	denotes	members
T11320	52886-52890	RB	denotes	also
T11316	52891-52901	VBP	denotes	contribute
T11321	52902-52904	IN	denotes	to
T11322	52905-52908	DT	denotes	the
T11324	52909-52913	JJ	denotes	down
T11325	52913-52914	HYPH	denotes	-
T11323	52914-52924	NN	denotes	regulation
T11326	52925-52927	IN	denotes	in
T11327	52928-52929	NN	denotes	E
T11329	52929-52930	HYPH	denotes	-
T11328	52930-52938	NN	denotes	cadherin
T11331	52939-52943	NN	denotes	gene
T11330	52944-52954	NN	denotes	expression
T11332	52955-52959	WDT	denotes	that
T11333	52960-52966	VBZ	denotes	occurs
T11334	52967-52973	IN	denotes	during
T11335	52974-52982	NN	denotes	follicle
T11336	52983-52992	NN	denotes	formation
T11337	52992-52993	.	denotes	.
T11338	52993-53162	sentence	denotes	However, it is intriguing that K14-Snail Tg epidermis displayed a marked down-regulation in E-cadherin expression, thereby demonstrating its potential to do so in skin.
T11339	52994-53001	RB	denotes	However
T11341	53001-53003	,	denotes	,
T11342	53003-53005	PRP	denotes	it
T11340	53006-53008	VBZ	denotes	is
T11343	53009-53019	VBG	denotes	intriguing
T11344	53020-53024	IN	denotes	that
T11346	53025-53028	NN	denotes	K14
T11348	53028-53029	HYPH	denotes	-
T11347	53029-53034	NN	denotes	Snail
T11350	53035-53037	NN	denotes	Tg
T11349	53038-53047	NN	denotes	epidermis
T11345	53048-53057	VBD	denotes	displayed
T11351	53058-53059	DT	denotes	a
T11353	53060-53066	JJ	denotes	marked
T11354	53067-53071	JJ	denotes	down
T11355	53071-53072	HYPH	denotes	-
T11352	53072-53082	NN	denotes	regulation
T11356	53083-53085	IN	denotes	in
T11357	53086-53087	NN	denotes	E
T11359	53087-53088	HYPH	denotes	-
T11358	53088-53096	NN	denotes	cadherin
T11360	53097-53107	NN	denotes	expression
T11361	53107-53109	,	denotes	,
T11362	53109-53116	RB	denotes	thereby
T11363	53117-53130	VBG	denotes	demonstrating
T11364	53131-53134	PRP$	denotes	its
T11365	53135-53144	NN	denotes	potential
T11366	53145-53147	TO	denotes	to
T11367	53148-53150	VB	denotes	do
T11368	53151-53153	RB	denotes	so
T11369	53154-53156	IN	denotes	in
T11370	53157-53161	NN	denotes	skin
T11371	53161-53162	.	denotes	.
T11372	53162-53315	sentence	denotes	Our prior findings showed that by elevating E-cadherin levels or by conditionally ablating α-catenin, hair follicle morphogenesis can be impaired [4,7].
T11373	53163-53166	PRP$	denotes	Our
T11375	53167-53172	JJ	denotes	prior
T11374	53173-53181	NNS	denotes	findings
T11376	53182-53188	VBD	denotes	showed
T11377	53189-53193	IN	denotes	that
T11379	53194-53196	IN	denotes	by
T11380	53197-53206	VBG	denotes	elevating
T11381	53207-53208	NN	denotes	E
T11383	53208-53209	HYPH	denotes	-
T11382	53209-53217	NN	denotes	cadherin
T11384	53218-53224	NNS	denotes	levels
T11385	53225-53227	CC	denotes	or
T11386	53228-53230	IN	denotes	by
T11387	53231-53244	RB	denotes	conditionally
T11388	53245-53253	VBG	denotes	ablating
T11389	53254-53255	NN	denotes	α
T11391	53255-53256	HYPH	denotes	-
T11390	53256-53263	NN	denotes	catenin
T11392	53263-53265	,	denotes	,
T11393	53265-53269	NN	denotes	hair
T11394	53270-53278	NN	denotes	follicle
T11395	53279-53292	NN	denotes	morphogenesis
T11396	53293-53296	MD	denotes	can
T11397	53297-53299	VB	denotes	be
T11378	53300-53308	VBN	denotes	impaired
T11398	53309-53310	-LRB-	denotes	[
T11400	53310-53311	CD	denotes	4
T11401	53311-53312	,	denotes	,
T11399	53312-53313	CD	denotes	7
T11402	53313-53314	-RRB-	denotes	]
T11403	53314-53315	.	denotes	.
T11404	53315-53645	sentence	denotes	The marked epidermal hyperproliferation seen in the K14-Snail Tg skin, coupled with the converse suppression of proliferation and Snail in TGF-β2-null hair buds led us to wonder whether the down-regulation of E-cadherin during follicle morphogenesis might have a direct impact on elevating the proliferative state of these cells.
T11405	53316-53319	DT	denotes	The
T11407	53320-53326	JJ	denotes	marked
T11408	53327-53336	JJ	denotes	epidermal
T11406	53337-53355	NN	denotes	hyperproliferation
T11410	53356-53360	VBN	denotes	seen
T11411	53361-53363	IN	denotes	in
T11412	53364-53367	DT	denotes	the
T11414	53368-53371	NN	denotes	K14
T11416	53371-53372	HYPH	denotes	-
T11415	53372-53377	NN	denotes	Snail
T11417	53378-53380	NN	denotes	Tg
T11413	53381-53385	NN	denotes	skin
T11418	53385-53387	,	denotes	,
T11419	53387-53394	VBN	denotes	coupled
T11420	53395-53399	IN	denotes	with
T11421	53400-53403	DT	denotes	the
T11423	53404-53412	JJ	denotes	converse
T11422	53413-53424	NN	denotes	suppression
T11424	53425-53427	IN	denotes	of
T11425	53428-53441	NN	denotes	proliferation
T11426	53442-53445	CC	denotes	and
T11427	53446-53451	NN	denotes	Snail
T11428	53452-53454	IN	denotes	in
T11429	53455-53458	NN	denotes	TGF
T11431	53458-53459	HYPH	denotes	-
T11430	53459-53461	NN	denotes	β2
T11433	53461-53462	HYPH	denotes	-
T11432	53462-53466	JJ	denotes	null
T11435	53467-53471	NN	denotes	hair
T11434	53472-53476	NNS	denotes	buds
T11409	53477-53480	VBD	denotes	led
T11436	53481-53483	PRP	denotes	us
T11437	53484-53486	TO	denotes	to
T11438	53487-53493	VB	denotes	wonder
T11439	53494-53501	IN	denotes	whether
T11441	53502-53505	DT	denotes	the
T11443	53506-53510	JJ	denotes	down
T11444	53510-53511	HYPH	denotes	-
T11442	53511-53521	NN	denotes	regulation
T11445	53522-53524	IN	denotes	of
T11446	53525-53526	NN	denotes	E
T11448	53526-53527	HYPH	denotes	-
T11447	53527-53535	NN	denotes	cadherin
T11449	53536-53542	IN	denotes	during
T11450	53543-53551	NN	denotes	follicle
T11451	53552-53565	NN	denotes	morphogenesis
T11452	53566-53571	MD	denotes	might
T11440	53572-53576	VB	denotes	have
T11453	53577-53578	DT	denotes	a
T11455	53579-53585	JJ	denotes	direct
T11454	53586-53592	NN	denotes	impact
T11456	53593-53595	IN	denotes	on
T11457	53596-53605	VBG	denotes	elevating
T11458	53606-53609	DT	denotes	the
T11460	53610-53623	JJ	denotes	proliferative
T11459	53624-53629	NN	denotes	state
T11461	53630-53632	IN	denotes	of
T11462	53633-53638	DT	denotes	these
T11463	53639-53644	NNS	denotes	cells
T11464	53644-53645	.	denotes	.
T11465	53645-53827	sentence	denotes	Our Tg studies suggested that, at least in part through its regulation of E-cadherin, Snail is able to influence the subcellular localization of a variety of AJ-associated proteins.
T11466	53646-53649	PRP$	denotes	Our
T11468	53650-53652	NN	denotes	Tg
T11467	53653-53660	NNS	denotes	studies
T11469	53661-53670	VBD	denotes	suggested
T11470	53671-53675	IN	denotes	that
T11472	53675-53677	,	denotes	,
T11473	53677-53679	RB	denotes	at
T11474	53680-53685	RBS	denotes	least
T11475	53686-53688	IN	denotes	in
T11477	53689-53693	NN	denotes	part
T11476	53694-53701	IN	denotes	through
T11478	53702-53705	PRP$	denotes	its
T11479	53706-53716	NN	denotes	regulation
T11480	53717-53719	IN	denotes	of
T11481	53720-53721	NN	denotes	E
T11483	53721-53722	HYPH	denotes	-
T11482	53722-53730	NN	denotes	cadherin
T11484	53730-53732	,	denotes	,
T11485	53732-53737	NN	denotes	Snail
T11471	53738-53740	VBZ	denotes	is
T11486	53741-53745	JJ	denotes	able
T11487	53746-53748	TO	denotes	to
T11488	53749-53758	VB	denotes	influence
T11489	53759-53762	DT	denotes	the
T11491	53763-53774	JJ	denotes	subcellular
T11490	53775-53787	NN	denotes	localization
T11492	53788-53790	IN	denotes	of
T11493	53791-53792	DT	denotes	a
T11494	53793-53800	NN	denotes	variety
T11495	53801-53803	IN	denotes	of
T11496	53804-53806	NN	denotes	AJ
T11498	53806-53807	HYPH	denotes	-
T11497	53807-53817	VBN	denotes	associated
T11499	53818-53826	NN	denotes	proteins
T11500	53826-53827	.	denotes	.
T11501	53827-53957	sentence	denotes	One of these appears to be Ajuba, which was previously shown to have the dual capacity to bind Grb-2 as well as α-catenin [9,10].
T11502	53828-53831	CD	denotes	One
T11504	53832-53834	IN	denotes	of
T11505	53835-53840	DT	denotes	these
T11503	53841-53848	VBZ	denotes	appears
T11506	53849-53851	TO	denotes	to
T11507	53852-53854	VB	denotes	be
T11508	53855-53860	NN	denotes	Ajuba
T11509	53860-53862	,	denotes	,
T11510	53862-53867	WDT	denotes	which
T11512	53868-53871	VBD	denotes	was
T11513	53872-53882	RB	denotes	previously
T11511	53883-53888	VBN	denotes	shown
T11514	53889-53891	TO	denotes	to
T11515	53892-53896	VB	denotes	have
T11516	53897-53900	DT	denotes	the
T11518	53901-53905	JJ	denotes	dual
T11517	53906-53914	NN	denotes	capacity
T11519	53915-53917	TO	denotes	to
T11520	53918-53922	VB	denotes	bind
T11521	53923-53926	NN	denotes	Grb
T11522	53926-53927	HYPH	denotes	-
T11523	53927-53928	CD	denotes	2
T11524	53929-53931	RB	denotes	as
T11526	53932-53936	RB	denotes	well
T11525	53937-53939	IN	denotes	as
T11527	53940-53941	NN	denotes	α
T11529	53941-53942	HYPH	denotes	-
T11528	53942-53949	NN	denotes	catenin
T11530	53950-53951	-LRB-	denotes	[
T11532	53951-53952	CD	denotes	9
T11533	53952-53953	,	denotes	,
T11531	53953-53955	CD	denotes	10
T11534	53955-53956	-RRB-	denotes	]
T11535	53956-53957	.	denotes	.
T11536	53957-54185	sentence	denotes	Our studies revealed that in skin keratinocytes that either harbor a conditional null mutation in α-catenin or that overexpress Snail, Ajuba develops an interaction with Grb-2 that is otherwise not observed in WT keratinocytes.
T11537	53958-53961	PRP$	denotes	Our
T11538	53962-53969	NNS	denotes	studies
T11539	53970-53978	VBD	denotes	revealed
T11540	53979-53983	IN	denotes	that
T11542	53984-53986	IN	denotes	in
T11543	53987-53991	NN	denotes	skin
T11544	53992-54005	NNS	denotes	keratinocytes
T11545	54006-54010	WDT	denotes	that
T11547	54011-54017	CC	denotes	either
T11546	54018-54024	VBP	denotes	harbor
T11548	54025-54026	DT	denotes	a
T11550	54027-54038	JJ	denotes	conditional
T11551	54039-54043	JJ	denotes	null
T11549	54044-54052	NN	denotes	mutation
T11552	54053-54055	IN	denotes	in
T11553	54056-54057	NN	denotes	α
T11555	54057-54058	HYPH	denotes	-
T11554	54058-54065	NN	denotes	catenin
T11556	54066-54068	CC	denotes	or
T11557	54069-54073	WDT	denotes	that
T11558	54074-54085	VBP	denotes	overexpress
T11559	54086-54091	NN	denotes	Snail
T11560	54091-54093	,	denotes	,
T11561	54093-54098	NN	denotes	Ajuba
T11541	54099-54107	VBZ	denotes	develops
T11562	54108-54110	DT	denotes	an
T11563	54111-54122	NN	denotes	interaction
T11564	54123-54127	IN	denotes	with
T11565	54128-54131	NN	denotes	Grb
T11566	54131-54132	HYPH	denotes	-
T11567	54132-54133	CD	denotes	2
T11568	54134-54138	WDT	denotes	that
T11570	54139-54141	VBZ	denotes	is
T11571	54142-54151	RB	denotes	otherwise
T11572	54152-54155	RB	denotes	not
T11569	54156-54164	VBN	denotes	observed
T11573	54165-54167	IN	denotes	in
T11574	54168-54170	NN	denotes	WT
T11575	54171-54184	NNS	denotes	keratinocytes
T11576	54184-54185	.	denotes	.
T11577	54185-54489	sentence	denotes	The corresponding abilities of either Snail-transfected or Ajuba-transfected keratinocytes to exhibit elevated activation of the Ras-MAPK pathway suggest that the Grb-2 association of Ajuba under conditions of reduced levels of AJ proteins may be directly relevant to the parallel in hyperproliferation.
T11578	54186-54189	DT	denotes	The
T11580	54190-54203	VBG	denotes	corresponding
T11579	54204-54213	NNS	denotes	abilities
T11582	54214-54216	IN	denotes	of
T11583	54217-54223	CC	denotes	either
T11585	54224-54229	NN	denotes	Snail
T11587	54229-54230	HYPH	denotes	-
T11586	54230-54241	VBN	denotes	transfected
T11588	54242-54244	CC	denotes	or
T11589	54245-54250	NN	denotes	Ajuba
T11591	54250-54251	HYPH	denotes	-
T11590	54251-54262	VBN	denotes	transfected
T11584	54263-54276	NNS	denotes	keratinocytes
T11592	54277-54279	TO	denotes	to
T11593	54280-54287	VB	denotes	exhibit
T11594	54288-54296	JJ	denotes	elevated
T11595	54297-54307	NN	denotes	activation
T11596	54308-54310	IN	denotes	of
T11597	54311-54314	DT	denotes	the
T11599	54315-54318	NN	denotes	Ras
T11601	54318-54319	HYPH	denotes	-
T11600	54319-54323	NN	denotes	MAPK
T11598	54324-54331	NN	denotes	pathway
T11581	54332-54339	VBP	denotes	suggest
T11602	54340-54344	IN	denotes	that
T11604	54345-54348	DT	denotes	the
T11606	54349-54352	NN	denotes	Grb
T11607	54352-54353	HYPH	denotes	-
T11608	54353-54354	CD	denotes	2
T11605	54355-54366	NN	denotes	association
T11609	54367-54369	IN	denotes	of
T11610	54370-54375	NN	denotes	Ajuba
T11611	54376-54381	IN	denotes	under
T11612	54382-54392	NNS	denotes	conditions
T11613	54393-54395	IN	denotes	of
T11614	54396-54403	JJ	denotes	reduced
T11615	54404-54410	NNS	denotes	levels
T11616	54411-54413	IN	denotes	of
T11617	54414-54416	NN	denotes	AJ
T11618	54417-54425	NN	denotes	proteins
T11619	54426-54429	MD	denotes	may
T11603	54430-54432	VB	denotes	be
T11620	54433-54441	RB	denotes	directly
T11621	54442-54450	JJ	denotes	relevant
T11622	54451-54453	IN	denotes	to
T11623	54454-54457	DT	denotes	the
T11624	54458-54466	NN	denotes	parallel
T11625	54467-54469	IN	denotes	in
T11626	54470-54488	NN	denotes	hyperproliferation
T11627	54488-54489	.	denotes	.
T11628	54489-54676	sentence	denotes	In stable epithelial (i.e., Madin-Darby canine kidney, or MDCK) cell lines, Snail has been shown to block cell cycle progression and promote motility and shape changes for invasion [47].
T11629	54490-54492	IN	denotes	In
T11631	54493-54499	JJ	denotes	stable
T11633	54500-54510	JJ	denotes	epithelial
T11634	54511-54512	-LRB-	denotes	(
T11636	54512-54516	FW	denotes	i.e.
T11637	54516-54518	,	denotes	,
T11638	54518-54523	NNP	denotes	Madin
T11640	54523-54524	HYPH	denotes	-
T11639	54524-54529	NNP	denotes	Darby
T11641	54530-54536	JJ	denotes	canine
T11635	54537-54543	NN	denotes	kidney
T11642	54543-54545	,	denotes	,
T11643	54545-54547	CC	denotes	or
T11644	54548-54552	NN	denotes	MDCK
T11645	54552-54553	-RRB-	denotes	)
T11646	54554-54558	NN	denotes	cell
T11632	54559-54564	NNS	denotes	lines
T11647	54564-54566	,	denotes	,
T11648	54566-54571	NN	denotes	Snail
T11649	54572-54575	VBZ	denotes	has
T11650	54576-54580	VBN	denotes	been
T11630	54581-54586	VBN	denotes	shown
T11651	54587-54589	TO	denotes	to
T11652	54590-54595	VB	denotes	block
T11653	54596-54600	NN	denotes	cell
T11654	54601-54606	NN	denotes	cycle
T11655	54607-54618	NN	denotes	progression
T11656	54619-54622	CC	denotes	and
T11657	54623-54630	VB	denotes	promote
T11658	54631-54639	NN	denotes	motility
T11660	54640-54643	CC	denotes	and
T11661	54644-54649	NN	denotes	shape
T11659	54650-54657	NNS	denotes	changes
T11662	54658-54661	IN	denotes	for
T11663	54662-54670	NN	denotes	invasion
T11664	54671-54672	-LRB-	denotes	[
T11665	54672-54674	CD	denotes	47
T11666	54674-54675	-RRB-	denotes	]
T11667	54675-54676	.	denotes	.
T11668	54676-54846	sentence	denotes	While our in vivo studies are consistent with a role for Snail in motility and epithelial remodeling, they differ with respect to Snail's apparent proliferative effects.
T11669	54677-54682	IN	denotes	While
T11671	54683-54686	PRP$	denotes	our
T11673	54687-54689	FW	denotes	in
T11674	54690-54694	FW	denotes	vivo
T11672	54695-54702	NNS	denotes	studies
T11670	54703-54706	VBP	denotes	are
T11676	54707-54717	JJ	denotes	consistent
T11677	54718-54722	IN	denotes	with
T11678	54723-54724	DT	denotes	a
T11679	54725-54729	NN	denotes	role
T11680	54730-54733	IN	denotes	for
T11681	54734-54739	NN	denotes	Snail
T11682	54740-54742	IN	denotes	in
T11683	54743-54751	NN	denotes	motility
T11685	54752-54755	CC	denotes	and
T11686	54756-54766	JJ	denotes	epithelial
T11684	54767-54777	NN	denotes	remodeling
T11687	54777-54779	,	denotes	,
T11688	54779-54783	PRP	denotes	they
T11675	54784-54790	VBP	denotes	differ
T11689	54791-54795	IN	denotes	with
T11690	54796-54803	NN	denotes	respect
T11691	54804-54806	IN	denotes	to
T11692	54807-54812	NN	denotes	Snail
T11694	54812-54814	POS	denotes	's
T11695	54815-54823	JJ	denotes	apparent
T11696	54824-54837	JJ	denotes	proliferative
T11693	54838-54845	NNS	denotes	effects
T11697	54845-54846	.	denotes	.
T11698	54846-54956	sentence	denotes	A priori, this could be simply due to variations in the response of different cell types to Snail expression.
T11699	54847-54848	FW	denotes	A
T11700	54849-54855	FW	denotes	priori
T11702	54855-54857	,	denotes	,
T11703	54857-54861	DT	denotes	this
T11704	54862-54867	MD	denotes	could
T11701	54868-54870	VB	denotes	be
T11705	54871-54877	RB	denotes	simply
T11706	54878-54881	IN	denotes	due
T11707	54882-54884	IN	denotes	to
T11708	54885-54895	NNS	denotes	variations
T11709	54896-54898	IN	denotes	in
T11710	54899-54902	DT	denotes	the
T11711	54903-54911	NN	denotes	response
T11712	54912-54914	IN	denotes	of
T11713	54915-54924	JJ	denotes	different
T11715	54925-54929	NN	denotes	cell
T11714	54930-54935	NNS	denotes	types
T11716	54936-54938	IN	denotes	to
T11717	54939-54944	NN	denotes	Snail
T11718	54945-54955	NN	denotes	expression
T11719	54955-54956	.	denotes	.
T11720	54956-55115	sentence	denotes	Alternatively, these differences may be relevant to the benefit of using mouse models to reveal functions not always recapitulated in stable cell line models.
T11721	54957-54970	RB	denotes	Alternatively
T11723	54970-54972	,	denotes	,
T11724	54972-54977	DT	denotes	these
T11725	54978-54989	NNS	denotes	differences
T11726	54990-54993	MD	denotes	may
T11722	54994-54996	VB	denotes	be
T11727	54997-55005	JJ	denotes	relevant
T11728	55006-55008	IN	denotes	to
T11729	55009-55012	DT	denotes	the
T11730	55013-55020	NN	denotes	benefit
T11731	55021-55023	IN	denotes	of
T11732	55024-55029	VBG	denotes	using
T11733	55030-55035	NN	denotes	mouse
T11734	55036-55042	NNS	denotes	models
T11735	55043-55045	TO	denotes	to
T11736	55046-55052	VB	denotes	reveal
T11737	55053-55062	NNS	denotes	functions
T11738	55063-55066	RB	denotes	not
T11740	55067-55073	RB	denotes	always
T11739	55074-55087	VBN	denotes	recapitulated
T11741	55088-55090	IN	denotes	in
T11742	55091-55097	JJ	denotes	stable
T11744	55098-55102	NN	denotes	cell
T11745	55103-55107	NN	denotes	line
T11743	55108-55114	NNS	denotes	models
T11746	55114-55115	.	denotes	.
T11747	55115-55198	sentence	denotes	Future studies should highlight the underlying reasons for these opposing results.
T11748	55116-55122	JJ	denotes	Future
T11749	55123-55130	NNS	denotes	studies
T11751	55131-55137	MD	denotes	should
T11750	55138-55147	VB	denotes	highlight
T11752	55148-55151	DT	denotes	the
T11754	55152-55162	JJ	denotes	underlying
T11753	55163-55170	NNS	denotes	reasons
T11755	55171-55174	IN	denotes	for
T11756	55175-55180	DT	denotes	these
T11758	55181-55189	VBG	denotes	opposing
T11757	55190-55197	NNS	denotes	results
T11759	55197-55198	.	denotes	.
T11760	55198-55383	sentence	denotes	Irrespective of these differences, our in vivo studies do not stand alone, as there are many situations in which a down-regulation in AJ proteins correlate with enhanced proliferation.
T11761	55199-55211	JJ	denotes	Irrespective
T11763	55212-55214	IN	denotes	of
T11764	55215-55220	DT	denotes	these
T11765	55221-55232	NNS	denotes	differences
T11766	55232-55234	,	denotes	,
T11767	55234-55237	PRP$	denotes	our
T11769	55238-55240	FW	denotes	in
T11770	55241-55245	FW	denotes	vivo
T11768	55246-55253	NNS	denotes	studies
T11771	55254-55256	VBP	denotes	do
T11772	55257-55260	RB	denotes	not
T11762	55261-55266	VB	denotes	stand
T11773	55267-55272	RB	denotes	alone
T11774	55272-55274	,	denotes	,
T11775	55274-55276	IN	denotes	as
T11777	55277-55282	EX	denotes	there
T11776	55283-55286	VBP	denotes	are
T11778	55287-55291	JJ	denotes	many
T11779	55292-55302	NNS	denotes	situations
T11780	55303-55305	IN	denotes	in
T11782	55306-55311	WDT	denotes	which
T11783	55312-55313	DT	denotes	a
T11785	55314-55318	JJ	denotes	down
T11786	55318-55319	HYPH	denotes	-
T11784	55319-55329	NN	denotes	regulation
T11787	55330-55332	IN	denotes	in
T11788	55333-55335	NN	denotes	AJ
T11789	55336-55344	NN	denotes	proteins
T11781	55345-55354	VBP	denotes	correlate
T11790	55355-55359	IN	denotes	with
T11791	55360-55368	VBN	denotes	enhanced
T11792	55369-55382	NN	denotes	proliferation
T11793	55382-55383	.	denotes	.
T11794	55383-55533	sentence	denotes	In fact, a myriad of diverse mechanisms have been implicated in activating epithelial proliferation upon down-regulation of AJ proteins [7,23,24,48].
T11795	55384-55386	IN	denotes	In
T11797	55387-55391	NN	denotes	fact
T11798	55391-55393	,	denotes	,
T11799	55393-55394	DT	denotes	a
T11800	55395-55401	NN	denotes	myriad
T11801	55402-55404	IN	denotes	of
T11802	55405-55412	JJ	denotes	diverse
T11803	55413-55423	NNS	denotes	mechanisms
T11804	55424-55428	VBP	denotes	have
T11805	55429-55433	VBN	denotes	been
T11796	55434-55444	VBN	denotes	implicated
T11806	55445-55447	IN	denotes	in
T11807	55448-55458	VBG	denotes	activating
T11808	55459-55469	JJ	denotes	epithelial
T11809	55470-55483	NN	denotes	proliferation
T11810	55484-55488	IN	denotes	upon
T11811	55489-55493	JJ	denotes	down
T11813	55493-55494	HYPH	denotes	-
T11812	55494-55504	NN	denotes	regulation
T11814	55505-55507	IN	denotes	of
T11815	55508-55510	NN	denotes	AJ
T11816	55511-55519	NN	denotes	proteins
T11817	55520-55521	-LRB-	denotes	[
T11819	55521-55522	CD	denotes	7
T11820	55522-55523	,	denotes	,
T11821	55523-55525	CD	denotes	23
T11822	55525-55526	,	denotes	,
T11823	55526-55528	CD	denotes	24
T11824	55528-55529	,	denotes	,
T11818	55529-55531	CD	denotes	48
T11825	55531-55532	-RRB-	denotes	]
T11826	55532-55533	.	denotes	.
T11827	55533-55628	sentence	denotes	Sifting through these converging pathways is likely to be a difficult and painstaking process.
T11828	55534-55541	VBG	denotes	Sifting
T11830	55542-55549	IN	denotes	through
T11831	55550-55555	DT	denotes	these
T11833	55556-55566	VBG	denotes	converging
T11832	55567-55575	NNS	denotes	pathways
T11829	55576-55578	VBZ	denotes	is
T11834	55579-55585	JJ	denotes	likely
T11835	55586-55588	TO	denotes	to
T11836	55589-55591	VB	denotes	be
T11837	55592-55593	DT	denotes	a
T11839	55594-55603	JJ	denotes	difficult
T11840	55604-55607	CC	denotes	and
T11841	55608-55619	JJ	denotes	painstaking
T11838	55620-55627	NN	denotes	process
T11842	55627-55628	.	denotes	.
T11843	55628-55913	sentence	denotes	This said, by identifying the status of different players involved in specific cell types and at specific stages in development, our mechanistic understanding of how intercellular remodeling is linked to proliferation in epithelial morphogenesis should begin to surface in the future.
T11844	55629-55633	DT	denotes	This
T11845	55634-55638	VBD	denotes	said
T11847	55638-55640	,	denotes	,
T11848	55640-55642	IN	denotes	by
T11849	55643-55654	VBG	denotes	identifying
T11850	55655-55658	DT	denotes	the
T11851	55659-55665	NN	denotes	status
T11852	55666-55668	IN	denotes	of
T11853	55669-55678	JJ	denotes	different
T11854	55679-55686	NNS	denotes	players
T11855	55687-55695	VBN	denotes	involved
T11856	55696-55698	IN	denotes	in
T11857	55699-55707	JJ	denotes	specific
T11859	55708-55712	NN	denotes	cell
T11858	55713-55718	NNS	denotes	types
T11860	55719-55722	CC	denotes	and
T11861	55723-55725	IN	denotes	at
T11862	55726-55734	JJ	denotes	specific
T11863	55735-55741	NNS	denotes	stages
T11864	55742-55744	IN	denotes	in
T11865	55745-55756	NN	denotes	development
T11866	55756-55758	,	denotes	,
T11867	55758-55761	PRP$	denotes	our
T11869	55762-55773	JJ	denotes	mechanistic
T11868	55774-55787	NN	denotes	understanding
T11870	55788-55790	IN	denotes	of
T11871	55791-55794	WRB	denotes	how
T11873	55795-55808	JJ	denotes	intercellular
T11874	55809-55819	NN	denotes	remodeling
T11875	55820-55822	VBZ	denotes	is
T11872	55823-55829	VBN	denotes	linked
T11876	55830-55832	IN	denotes	to
T11877	55833-55846	NN	denotes	proliferation
T11878	55847-55849	IN	denotes	in
T11879	55850-55860	JJ	denotes	epithelial
T11880	55861-55874	NN	denotes	morphogenesis
T11881	55875-55881	MD	denotes	should
T11846	55882-55887	VB	denotes	begin
T11882	55888-55890	TO	denotes	to
T11883	55891-55898	VB	denotes	surface
T11884	55899-55901	IN	denotes	in
T11885	55902-55905	DT	denotes	the
T11886	55906-55912	NN	denotes	future
T11887	55912-55913	.	denotes	.
T11888	55913-56079	sentence	denotes	Elucidating the molecular mechanisms through which these networks converge is also a prerequisite for understanding how these processes go awry during tumorigenesis.
T11889	55914-55925	VBG	denotes	Elucidating
T11891	55926-55929	DT	denotes	the
T11893	55930-55939	JJ	denotes	molecular
T11892	55940-55950	NNS	denotes	mechanisms
T11894	55951-55958	IN	denotes	through
T11896	55959-55964	WDT	denotes	which
T11897	55965-55970	DT	denotes	these
T11898	55971-55979	NNS	denotes	networks
T11895	55980-55988	VBP	denotes	converge
T11890	55989-55991	VBZ	denotes	is
T11899	55992-55996	RB	denotes	also
T11900	55997-55998	DT	denotes	a
T11901	55999-56011	NN	denotes	prerequisite
T11902	56012-56015	IN	denotes	for
T11903	56016-56029	VBG	denotes	understanding
T11904	56030-56033	WRB	denotes	how
T11906	56034-56039	DT	denotes	these
T11907	56040-56049	NNS	denotes	processes
T11905	56050-56052	VBP	denotes	go
T11908	56053-56057	RB	denotes	awry
T11909	56058-56064	IN	denotes	during
T11910	56065-56078	NN	denotes	tumorigenesis
T11911	56078-56079	.	denotes	.
T12021	56104-56112	NNS	denotes	Reagents
T12022	56112-57203	sentence	denotes	Primary antibodies used were against: E-cadherin (M. Takeichi, Kyoto University, Japan); α-catenin, β-catenin, pMAPK, tubulin (Sigma, St. Louis, Missouri, United States), Ajuba (G. Longmore, Washington University, St. Louis, Missouri, United States); β4 integrin/CD104 (BD Pharmingen, San Diego, California, United States), laminin 5 (R. Burgeson, Harvard University, Cambridge, Massachusetts, United States), K5, K1, loricrin (Fuchs Lab), involucrin, fillagrin (Covance, Berkeley, California, United States), MAPK, pSMAD2 (Cell Signaling, Beverly, Massachusetts, United States); Grb-2 (Santa Cruz Biotech, Santa Cruz, California, United States); P-cadherin (Zymed Laboratories, South San Francisco, California, United States); HA (Roche Biochemicals), vimentin (Chemicon, Temecula, California, United States), Ki67 (Novo Castra, Newcastle Upon Tyne, United Kingdom), keratin 6 (P. Coulombe, John Hopkins University, Baltimore, Maryland, United States), cyclin D (Oncogene, San Diego, California, United States), and TGF-β2 (L. Gold, New York University, New York, New York, United States).
T12023	56113-56120	JJ	denotes	Primary
T12024	56121-56131	NNS	denotes	antibodies
T12026	56132-56136	VBN	denotes	used
T12025	56137-56141	VBD	denotes	were
T12027	56142-56149	IN	denotes	against
T12028	56149-56151	:	denotes	:
T12029	56151-56152	NN	denotes	E
T12031	56152-56153	HYPH	denotes	-
T12030	56153-56161	NN	denotes	cadherin
T12032	56162-56163	-LRB-	denotes	(
T12034	56163-56165	NNP	denotes	M.
T12033	56166-56174	NNP	denotes	Takeichi
T12035	56174-56176	,	denotes	,
T12036	56176-56181	NNP	denotes	Kyoto
T12037	56182-56192	NNP	denotes	University
T12038	56192-56194	,	denotes	,
T12039	56194-56199	NNP	denotes	Japan
T12040	56199-56200	-RRB-	denotes	)
T12041	56200-56201	:	denotes	;
T12042	56202-56203	NN	denotes	α
T12044	56203-56204	HYPH	denotes	-
T12043	56204-56211	NN	denotes	catenin
T12045	56211-56213	,	denotes	,
T12046	56213-56214	NN	denotes	β
T12048	56214-56215	HYPH	denotes	-
T12047	56215-56222	NN	denotes	catenin
T12049	56222-56224	,	denotes	,
T12050	56224-56229	NN	denotes	pMAPK
T12051	56229-56231	,	denotes	,
T12052	56231-56238	NN	denotes	tubulin
T12053	56239-56240	-LRB-	denotes	(
T12054	56240-56245	NNP	denotes	Sigma
T12055	56245-56247	,	denotes	,
T12056	56247-56250	NNP	denotes	St.
T12057	56251-56256	NNP	denotes	Louis
T12058	56256-56258	,	denotes	,
T12059	56258-56266	NNP	denotes	Missouri
T12060	56266-56268	,	denotes	,
T12061	56268-56274	NNP	denotes	United
T12062	56275-56281	NNP	denotes	States
T12063	56281-56282	-RRB-	denotes	)
T12064	56282-56284	,	denotes	,
T12065	56284-56289	NN	denotes	Ajuba
T12066	56290-56291	-LRB-	denotes	(
T12068	56291-56293	NNP	denotes	G.
T12067	56294-56302	NNP	denotes	Longmore
T12069	56302-56304	,	denotes	,
T12070	56304-56314	NNP	denotes	Washington
T12071	56315-56325	NNP	denotes	University
T12072	56325-56327	,	denotes	,
T12073	56327-56330	NNP	denotes	St.
T12074	56331-56336	NNP	denotes	Louis
T12075	56336-56338	,	denotes	,
T12076	56338-56346	NNP	denotes	Missouri
T12077	56346-56348	,	denotes	,
T12078	56348-56354	NNP	denotes	United
T12079	56355-56361	NNP	denotes	States
T12080	56361-56362	-RRB-	denotes	)
T12081	56362-56363	:	denotes	;
T12082	56364-56366	NN	denotes	β4
T12083	56367-56375	NN	denotes	integrin
T12084	56375-56376	HYPH	denotes	/
T12085	56376-56381	NN	denotes	CD104
T12086	56382-56383	-LRB-	denotes	(
T12088	56383-56385	NNP	denotes	BD
T12087	56386-56396	NNP	denotes	Pharmingen
T12089	56396-56398	,	denotes	,
T12090	56398-56401	NNP	denotes	San
T12091	56402-56407	NNP	denotes	Diego
T12092	56407-56409	,	denotes	,
T12093	56409-56419	NNP	denotes	California
T12094	56419-56421	,	denotes	,
T12095	56421-56427	NNP	denotes	United
T12096	56428-56434	NNP	denotes	States
T12097	56434-56435	-RRB-	denotes	)
T12098	56435-56437	,	denotes	,
T12099	56437-56444	NN	denotes	laminin
T12100	56445-56446	CD	denotes	5
T12101	56447-56448	-LRB-	denotes	(
T12103	56448-56450	NNP	denotes	R.
T12102	56451-56459	NNP	denotes	Burgeson
T12104	56459-56461	,	denotes	,
T12105	56461-56468	NNP	denotes	Harvard
T12106	56469-56479	NNP	denotes	University
T12107	56479-56481	,	denotes	,
T12108	56481-56490	NNP	denotes	Cambridge
T12109	56490-56492	,	denotes	,
T12110	56492-56505	NNP	denotes	Massachusetts
T12111	56505-56507	,	denotes	,
T12112	56507-56513	NNP	denotes	United
T12113	56514-56520	NNP	denotes	States
T12114	56520-56521	-RRB-	denotes	)
T12115	56521-56523	,	denotes	,
T12116	56523-56525	NN	denotes	K5
T12117	56525-56527	,	denotes	,
T12118	56527-56529	NN	denotes	K1
T12119	56529-56531	,	denotes	,
T12120	56531-56539	NN	denotes	loricrin
T12121	56540-56541	-LRB-	denotes	(
T12123	56541-56546	NNP	denotes	Fuchs
T12122	56547-56550	NNP	denotes	Lab
T12124	56550-56551	-RRB-	denotes	)
T12125	56551-56553	,	denotes	,
T12126	56553-56563	NN	denotes	involucrin
T12127	56563-56565	,	denotes	,
T12128	56565-56574	NN	denotes	fillagrin
T12129	56575-56576	-LRB-	denotes	(
T12130	56576-56583	NNP	denotes	Covance
T12131	56583-56585	,	denotes	,
T12132	56585-56593	NNP	denotes	Berkeley
T12133	56593-56595	,	denotes	,
T12134	56595-56605	NNP	denotes	California
T12135	56605-56607	,	denotes	,
T12136	56607-56613	NNP	denotes	United
T12137	56614-56620	NNP	denotes	States
T12138	56620-56621	-RRB-	denotes	)
T12139	56621-56623	,	denotes	,
T12140	56623-56627	NN	denotes	MAPK
T12141	56627-56629	,	denotes	,
T12142	56629-56635	NN	denotes	pSMAD2
T12143	56636-56637	-LRB-	denotes	(
T12145	56637-56641	NNP	denotes	Cell
T12144	56642-56651	NNP	denotes	Signaling
T12146	56651-56653	,	denotes	,
T12147	56653-56660	NNP	denotes	Beverly
T12148	56660-56662	,	denotes	,
T12149	56662-56675	NNP	denotes	Massachusetts
T12150	56675-56677	,	denotes	,
T12151	56677-56683	NNP	denotes	United
T12152	56684-56690	NNP	denotes	States
T12153	56690-56691	-RRB-	denotes	)
T12154	56691-56692	:	denotes	;
T12155	56693-56696	NN	denotes	Grb
T12156	56696-56697	HYPH	denotes	-
T12157	56697-56698	CD	denotes	2
T12158	56699-56700	-LRB-	denotes	(
T12160	56700-56705	NNP	denotes	Santa
T12161	56706-56710	NNP	denotes	Cruz
T12159	56711-56718	NNP	denotes	Biotech
T12162	56718-56720	,	denotes	,
T12163	56720-56725	NNP	denotes	Santa
T12164	56726-56730	NNP	denotes	Cruz
T12165	56730-56732	,	denotes	,
T12166	56732-56742	NNP	denotes	California
T12167	56742-56744	,	denotes	,
T12168	56744-56750	NNP	denotes	United
T12169	56751-56757	NNP	denotes	States
T12170	56757-56758	-RRB-	denotes	)
T12171	56758-56759	:	denotes	;
T12172	56760-56761	NN	denotes	P
T12174	56761-56762	HYPH	denotes	-
T12173	56762-56770	NN	denotes	cadherin
T12175	56771-56772	-LRB-	denotes	(
T12177	56772-56777	NNP	denotes	Zymed
T12176	56778-56790	NNP	denotes	Laboratories
T12178	56790-56792	,	denotes	,
T12179	56792-56797	JJ	denotes	South
T12181	56798-56801	NNP	denotes	San
T12180	56802-56811	NNP	denotes	Francisco
T12182	56811-56813	,	denotes	,
T12183	56813-56823	NNP	denotes	California
T12184	56823-56825	,	denotes	,
T12185	56825-56831	NNP	denotes	United
T12186	56832-56838	NNP	denotes	States
T12187	56838-56839	-RRB-	denotes	)
T12188	56839-56840	:	denotes	;
T12189	56841-56843	NN	denotes	HA
T12190	56844-56845	-LRB-	denotes	(
T12192	56845-56850	NNP	denotes	Roche
T12191	56851-56863	NNP	denotes	Biochemicals
T12193	56863-56864	-RRB-	denotes	)
T12194	56864-56866	,	denotes	,
T12195	56866-56874	NN	denotes	vimentin
T12196	56875-56876	-LRB-	denotes	(
T12197	56876-56884	NNP	denotes	Chemicon
T12198	56884-56886	,	denotes	,
T12199	56886-56894	NNP	denotes	Temecula
T12200	56894-56896	,	denotes	,
T12201	56896-56906	NNP	denotes	California
T12202	56906-56908	,	denotes	,
T12203	56908-56914	NNP	denotes	United
T12204	56915-56921	NNP	denotes	States
T12205	56921-56922	-RRB-	denotes	)
T12206	56922-56924	,	denotes	,
T12207	56924-56928	NN	denotes	Ki67
T12208	56929-56930	-LRB-	denotes	(
T12210	56930-56934	NNP	denotes	Novo
T12209	56935-56941	NNP	denotes	Castra
T12211	56941-56943	,	denotes	,
T12212	56943-56952	NNP	denotes	Newcastle
T12213	56953-56957	IN	denotes	Upon
T12214	56958-56962	NNP	denotes	Tyne
T12215	56962-56964	,	denotes	,
T12216	56964-56970	NNP	denotes	United
T12217	56971-56978	NNP	denotes	Kingdom
T12218	56978-56979	-RRB-	denotes	)
T12219	56979-56981	,	denotes	,
T12220	56981-56988	NN	denotes	keratin
T12221	56989-56990	CD	denotes	6
T12222	56991-56992	-LRB-	denotes	(
T12224	56992-56994	NNP	denotes	P.
T12223	56995-57003	NNP	denotes	Coulombe
T12225	57003-57005	,	denotes	,
T12226	57005-57009	NNP	denotes	John
T12227	57010-57017	NNP	denotes	Hopkins
T12228	57018-57028	NNP	denotes	University
T12229	57028-57030	,	denotes	,
T12230	57030-57039	NNP	denotes	Baltimore
T12231	57039-57041	,	denotes	,
T12232	57041-57049	NNP	denotes	Maryland
T12233	57049-57051	,	denotes	,
T12234	57051-57057	NNP	denotes	United
T12235	57058-57064	NNP	denotes	States
T12236	57064-57065	-RRB-	denotes	)
T12237	57065-57067	,	denotes	,
T12238	57067-57073	NN	denotes	cyclin
T12239	57074-57075	NN	denotes	D
T12240	57076-57077	-LRB-	denotes	(
T12241	57077-57085	NNP	denotes	Oncogene
T12242	57085-57087	,	denotes	,
T12243	57087-57090	NNP	denotes	San
T12244	57091-57096	NNP	denotes	Diego
T12245	57096-57098	,	denotes	,
T12246	57098-57108	NNP	denotes	California
T12247	57108-57110	,	denotes	,
T12248	57110-57116	NNP	denotes	United
T12249	57117-57123	NNP	denotes	States
T12250	57123-57124	-RRB-	denotes	)
T12251	57124-57126	,	denotes	,
T12252	57126-57129	CC	denotes	and
T12253	57130-57133	NN	denotes	TGF
T12255	57133-57134	HYPH	denotes	-
T12254	57134-57136	NN	denotes	β2
T12256	57137-57138	-LRB-	denotes	(
T12258	57138-57140	NNP	denotes	L.
T12257	57141-57145	NNP	denotes	Gold
T12259	57145-57147	,	denotes	,
T12260	57147-57150	NNP	denotes	New
T12261	57151-57155	NNP	denotes	York
T12262	57156-57166	NNP	denotes	University
T12263	57166-57168	,	denotes	,
T12264	57168-57171	NNP	denotes	New
T12265	57172-57176	NNP	denotes	York
T12266	57176-57178	,	denotes	,
T12267	57178-57181	NNP	denotes	New
T12268	57182-57186	NNP	denotes	York
T12269	57186-57188	,	denotes	,
T12270	57188-57194	NNP	denotes	United
T12271	57195-57201	NNP	denotes	States
T12272	57201-57202	-RRB-	denotes	)
T12273	57202-57203	.	denotes	.
T12274	57203-57337	sentence	denotes	FITC-, Texas Red-, or HRP-conjugated secondary antibodies were from Jackson ImmunoResearch (West Grove, Pennsylvania, United States).
T12275	57204-57208	NN	denotes	FITC
T12277	57208-57209	HYPH	denotes	-
T12278	57209-57211	,	denotes	,
T12279	57211-57216	NNP	denotes	Texas
T12280	57217-57220	NNP	denotes	Red
T12281	57220-57221	HYPH	denotes	-
T12282	57221-57223	,	denotes	,
T12283	57223-57225	CC	denotes	or
T12284	57226-57229	NN	denotes	HRP
T12285	57229-57230	HYPH	denotes	-
T12276	57230-57240	VBN	denotes	conjugated
T12287	57241-57250	JJ	denotes	secondary
T12286	57251-57261	NNS	denotes	antibodies
T12288	57262-57266	VBD	denotes	were
T12289	57267-57271	IN	denotes	from
T12290	57272-57279	NNP	denotes	Jackson
T12291	57280-57294	NNP	denotes	ImmunoResearch
T12292	57295-57296	-LRB-	denotes	(
T12294	57296-57300	NNP	denotes	West
T12293	57301-57306	NNP	denotes	Grove
T12295	57306-57308	,	denotes	,
T12296	57308-57320	NNP	denotes	Pennsylvania
T12297	57320-57322	,	denotes	,
T12298	57322-57328	NNP	denotes	United
T12299	57329-57335	NNP	denotes	States
T12300	57335-57336	-RRB-	denotes	)
T12301	57336-57337	.	denotes	.
T12302	57337-57434	sentence	denotes	Biotinylated secondary antibodies were from Vector Labs (Burlingame, California, United States).
T12303	57338-57350	VBN	denotes	Biotinylated
T12305	57351-57360	JJ	denotes	secondary
T12304	57361-57371	NNS	denotes	antibodies
T12306	57372-57376	VBD	denotes	were
T12307	57377-57381	IN	denotes	from
T12308	57382-57388	NNP	denotes	Vector
T12309	57389-57393	NNP	denotes	Labs
T12310	57394-57395	-LRB-	denotes	(
T12311	57395-57405	NNP	denotes	Burlingame
T12312	57405-57407	,	denotes	,
T12313	57407-57417	NNP	denotes	California
T12314	57417-57419	,	denotes	,
T12315	57419-57425	NNP	denotes	United
T12316	57426-57432	NNP	denotes	States
T12317	57432-57433	-RRB-	denotes	)
T12318	57433-57434	.	denotes	.
T12319	57434-57497	sentence	denotes	Dilutions were according to the manufacturer's recommendation.
T12320	57435-57444	NNS	denotes	Dilutions
T12321	57445-57449	VBD	denotes	were
T12322	57450-57459	VBG	denotes	according
T12323	57460-57462	IN	denotes	to
T12324	57463-57466	DT	denotes	the
T12325	57467-57479	NN	denotes	manufacturer
T12327	57479-57481	POS	denotes	's
T12326	57482-57496	NN	denotes	recommendation
T12328	57496-57497	.	denotes	.
T12329	57497-57672	sentence	denotes	The Snail antibody was generated in Guinea pigs by inoculating them with the N-terminal sequence of murine Snail fused to GST (Covance, Princeton, New Jersey, United States).
T12330	57498-57501	DT	denotes	The
T12332	57502-57507	NN	denotes	Snail
T12331	57508-57516	NN	denotes	antibody
T12334	57517-57520	VBD	denotes	was
T12333	57521-57530	VBN	denotes	generated
T12335	57531-57533	IN	denotes	in
T12336	57534-57540	NN	denotes	Guinea
T12337	57541-57545	NNS	denotes	pigs
T12338	57546-57548	IN	denotes	by
T12339	57549-57560	VBG	denotes	inoculating
T12340	57561-57565	PRP	denotes	them
T12341	57566-57570	IN	denotes	with
T12342	57571-57574	DT	denotes	the
T12344	57575-57576	NN	denotes	N
T12346	57576-57577	HYPH	denotes	-
T12345	57577-57585	JJ	denotes	terminal
T12343	57586-57594	NN	denotes	sequence
T12347	57595-57597	IN	denotes	of
T12348	57598-57604	JJ	denotes	murine
T12349	57605-57610	NN	denotes	Snail
T12350	57611-57616	VBN	denotes	fused
T12351	57617-57619	IN	denotes	to
T12352	57620-57623	NN	denotes	GST
T12353	57624-57625	-LRB-	denotes	(
T12354	57625-57632	NNP	denotes	Covance
T12355	57632-57634	,	denotes	,
T12356	57634-57643	NNP	denotes	Princeton
T12357	57643-57645	,	denotes	,
T12358	57645-57648	NNP	denotes	New
T12359	57649-57655	NNP	denotes	Jersey
T12360	57655-57657	,	denotes	,
T12361	57657-57663	NNP	denotes	United
T12362	57664-57670	NNP	denotes	States
T12363	57670-57671	-RRB-	denotes	)
T12364	57671-57672	.	denotes	.
T12365	57672-57761	sentence	denotes	Recombinant human TGF-β2 was purchased from R&D (Minneapolis, Minnesota, United States).
T12366	57673-57684	JJ	denotes	Recombinant
T12368	57685-57690	JJ	denotes	human
T12369	57691-57694	NN	denotes	TGF
T12370	57694-57695	HYPH	denotes	-
T12367	57695-57697	NN	denotes	β2
T12372	57698-57701	VBD	denotes	was
T12371	57702-57711	VBN	denotes	purchased
T12373	57712-57716	IN	denotes	from
T12374	57717-57718	NNP	denotes	R
T12375	57718-57719	CC	denotes	&
T12376	57719-57720	NNP	denotes	D
T12377	57721-57722	-LRB-	denotes	(
T12378	57722-57733	NNP	denotes	Minneapolis
T12379	57733-57735	,	denotes	,
T12380	57735-57744	NNP	denotes	Minnesota
T12381	57744-57746	,	denotes	,
T12382	57746-57752	NNP	denotes	United
T12383	57753-57759	NNP	denotes	States
T12384	57759-57760	-RRB-	denotes	)
T12385	57760-57761	.	denotes	.
T12386	57761-57856	sentence	denotes	Heat inactivated TGF-β2 was generated by heating the recombinant protein at 100 °C for 10 min.
T12387	57762-57766	NN	denotes	Heat
T12388	57767-57778	VBN	denotes	inactivated
T12390	57779-57782	NN	denotes	TGF
T12391	57782-57783	HYPH	denotes	-
T12389	57783-57785	NN	denotes	β2
T12393	57786-57789	VBD	denotes	was
T12392	57790-57799	VBN	denotes	generated
T12394	57800-57802	IN	denotes	by
T12395	57803-57810	VBG	denotes	heating
T12396	57811-57814	DT	denotes	the
T12398	57815-57826	JJ	denotes	recombinant
T12397	57827-57834	NN	denotes	protein
T12399	57835-57837	IN	denotes	at
T12400	57838-57841	CD	denotes	100
T12401	57842-57844	NN	denotes	°C
T12402	57845-57848	IN	denotes	for
T12403	57849-57851	CD	denotes	10
T12404	57852-57855	NN	denotes	min
T12405	57855-57856	.	denotes	.
T12464	57858-57862	NNS	denotes	Mice
T12465	57862-58065	sentence	denotes	The K14-Snail Tg mouse was generated by digesting the pcDNA3-mm Snail-HA plasmid (G. de Herreros, Universitat Pompeu, Fabra, Barcelona, Spain) with BamHI and NotI and subcloned into the K14 vector [49].
T12466	57863-57866	DT	denotes	The
T12468	57867-57870	NN	denotes	K14
T12470	57870-57871	HYPH	denotes	-
T12469	57871-57876	NN	denotes	Snail
T12471	57877-57879	NN	denotes	Tg
T12467	57880-57885	NN	denotes	mouse
T12473	57886-57889	VBD	denotes	was
T12472	57890-57899	VBN	denotes	generated
T12474	57900-57902	IN	denotes	by
T12475	57903-57912	VBG	denotes	digesting
T12476	57913-57916	DT	denotes	the
T12478	57917-57923	NN	denotes	pcDNA3
T12480	57923-57924	HYPH	denotes	-
T12479	57924-57926	NN	denotes	mm
T12481	57927-57932	NN	denotes	Snail
T12483	57932-57933	HYPH	denotes	-
T12482	57933-57935	NN	denotes	HA
T12477	57936-57943	NN	denotes	plasmid
T12484	57944-57945	-LRB-	denotes	(
T12486	57945-57947	NNP	denotes	G.
T12487	57948-57950	NNP	denotes	de
T12485	57951-57959	NNP	denotes	Herreros
T12488	57959-57961	,	denotes	,
T12489	57961-57972	NNP	denotes	Universitat
T12490	57973-57979	NNP	denotes	Pompeu
T12491	57979-57981	,	denotes	,
T12492	57981-57986	NNP	denotes	Fabra
T12493	57986-57988	,	denotes	,
T12494	57988-57997	NNP	denotes	Barcelona
T12495	57997-57999	,	denotes	,
T12496	57999-58004	NNP	denotes	Spain
T12497	58004-58005	-RRB-	denotes	)
T12498	58006-58010	IN	denotes	with
T12499	58011-58016	NN	denotes	BamHI
T12500	58017-58020	CC	denotes	and
T12501	58021-58025	NN	denotes	NotI
T12502	58026-58029	CC	denotes	and
T12503	58030-58039	VBN	denotes	subcloned
T12504	58040-58044	IN	denotes	into
T12505	58045-58048	DT	denotes	the
T12507	58049-58052	NN	denotes	K14
T12506	58053-58059	NN	denotes	vector
T12508	58060-58061	-LRB-	denotes	[
T12509	58061-58063	CD	denotes	49
T12510	58063-58064	-RRB-	denotes	]
T12511	58064-58065	.	denotes	.
T12512	58065-58146	sentence	denotes	The linearized construct was injected into the nucleus of embryos from CD1 mice.
T12513	58066-58069	DT	denotes	The
T12515	58070-58080	VBN	denotes	linearized
T12514	58081-58090	NN	denotes	construct
T12517	58091-58094	VBD	denotes	was
T12516	58095-58103	VBN	denotes	injected
T12518	58104-58108	IN	denotes	into
T12519	58109-58112	DT	denotes	the
T12520	58113-58120	NN	denotes	nucleus
T12521	58121-58123	IN	denotes	of
T12522	58124-58131	NNS	denotes	embryos
T12523	58132-58136	IN	denotes	from
T12524	58137-58140	NN	denotes	CD1
T12525	58141-58145	NNS	denotes	mice
T12526	58145-58146	.	denotes	.
T12527	58146-58204	sentence	denotes	The K14-Smad 2 Tg mouse was reported in Ito et al., 2001.
T12528	58147-58150	DT	denotes	The
T12530	58151-58154	NN	denotes	K14
T12532	58154-58155	HYPH	denotes	-
T12531	58155-58159	NN	denotes	Smad
T12533	58160-58161	CD	denotes	2
T12534	58162-58164	NN	denotes	Tg
T12529	58165-58170	NN	denotes	mouse
T12536	58171-58174	VBD	denotes	was
T12535	58175-58183	VBN	denotes	reported
T12537	58184-58186	IN	denotes	in
T12538	58187-58190	NNP	denotes	Ito
T12539	58191-58193	FW	denotes	et
T12540	58194-58197	FW	denotes	al.
T12541	58197-58199	,	denotes	,
T12542	58199-58203	CD	denotes	2001
T12543	58203-58204	.	denotes	.
T12544	58204-58258	sentence	denotes	The TGF-β2 knockout (KO) mouse was described in [34].
T12545	58205-58208	DT	denotes	The
T12547	58209-58212	NN	denotes	TGF
T12549	58212-58213	HYPH	denotes	-
T12548	58213-58215	NN	denotes	β2
T12550	58216-58224	NN	denotes	knockout
T12551	58225-58226	-LRB-	denotes	(
T12552	58226-58228	NN	denotes	KO
T12553	58228-58229	-RRB-	denotes	)
T12546	58230-58235	NN	denotes	mouse
T12555	58236-58239	VBD	denotes	was
T12554	58240-58249	VBN	denotes	described
T12556	58250-58252	IN	denotes	in
T12557	58253-58254	-LRB-	denotes	[
T12558	58254-58256	NN	denotes	34
T12559	58256-58257	-RRB-	denotes	]
T12560	58257-58258	.	denotes	.
T12561	58258-58333	sentence	denotes	The shh KO mouse [38] and TOPGal mouse [20] have previously been reported.
T12562	58259-58262	DT	denotes	The
T12564	58263-58266	NN	denotes	shh
T12565	58267-58269	NN	denotes	KO
T12563	58270-58275	NN	denotes	mouse
T12567	58276-58277	-LRB-	denotes	[
T12568	58277-58279	CD	denotes	38
T12569	58279-58280	-RRB-	denotes	]
T12570	58281-58284	CC	denotes	and
T12571	58285-58291	NN	denotes	TOPGal
T12572	58292-58297	NN	denotes	mouse
T12573	58298-58299	-LRB-	denotes	[
T12574	58299-58301	CD	denotes	20
T12575	58301-58302	-RRB-	denotes	]
T12576	58303-58307	VBP	denotes	have
T12577	58308-58318	RB	denotes	previously
T12578	58319-58323	VBN	denotes	been
T12566	58324-58332	VBN	denotes	reported
T12579	58332-58333	.	denotes	.
T12687	58335-58342	NNP	denotes	Western
T12688	58343-58347	NN	denotes	blot
T12689	58348-58351	CC	denotes	and
T12690	58352-58371	NN	denotes	immunoprecipitation
T12691	58371-58677	sentence	denotes	Protein extracts from primary keratinocytes were generated either by lysing cells in lysis buffer (1% NP-40, 1% sodium deoxycholate, 20 mM Tris-Cl [pH 7.4], 140 mM NaCl containing 1 mM sodium vanadate, 2 mM phenylmethylsulfonyl fluoride, and protease inhibitors) or directly in Laemmli bβuffer and boiled.
T12692	58372-58379	NN	denotes	Protein
T12693	58380-58388	NNS	denotes	extracts
T12695	58389-58393	IN	denotes	from
T12696	58394-58401	JJ	denotes	primary
T12697	58402-58415	NNS	denotes	keratinocytes
T12698	58416-58420	VBD	denotes	were
T12694	58421-58430	VBN	denotes	generated
T12699	58431-58437	CC	denotes	either
T12700	58438-58440	IN	denotes	by
T12701	58441-58447	VBG	denotes	lysing
T12702	58448-58453	NNS	denotes	cells
T12703	58454-58456	IN	denotes	in
T12704	58457-58462	NN	denotes	lysis
T12705	58463-58469	NN	denotes	buffer
T12706	58470-58471	-LRB-	denotes	(
T12708	58471-58472	CD	denotes	1
T12709	58472-58473	NN	denotes	%
T12707	58474-58476	NN	denotes	NP
T12710	58476-58477	HYPH	denotes	-
T12711	58477-58479	CD	denotes	40
T12712	58479-58481	,	denotes	,
T12713	58481-58482	CD	denotes	1
T12714	58482-58483	NN	denotes	%
T12716	58484-58490	NN	denotes	sodium
T12715	58491-58503	NN	denotes	deoxycholate
T12717	58503-58505	,	denotes	,
T12718	58505-58507	CD	denotes	20
T12719	58508-58510	NN	denotes	mM
T12721	58511-58515	NN	denotes	Tris
T12722	58515-58516	HYPH	denotes	-
T12720	58516-58518	NN	denotes	Cl
T12723	58519-58520	-LRB-	denotes	[
T12724	58520-58522	NN	denotes	pH
T12725	58523-58526	CD	denotes	7.4
T12726	58526-58527	-RRB-	denotes	]
T12727	58527-58529	,	denotes	,
T12728	58529-58532	CD	denotes	140
T12729	58533-58535	NN	denotes	mM
T12730	58536-58540	NN	denotes	NaCl
T12731	58541-58551	VBG	denotes	containing
T12732	58552-58553	CD	denotes	1
T12733	58554-58556	NN	denotes	mM
T12735	58557-58563	NN	denotes	sodium
T12734	58564-58572	NN	denotes	vanadate
T12736	58572-58574	,	denotes	,
T12737	58574-58575	CD	denotes	2
T12738	58576-58578	NN	denotes	mM
T12740	58579-58599	NN	denotes	phenylmethylsulfonyl
T12739	58600-58608	NN	denotes	fluoride
T12741	58608-58610	,	denotes	,
T12742	58610-58613	CC	denotes	and
T12743	58614-58622	NN	denotes	protease
T12744	58623-58633	NNS	denotes	inhibitors
T12745	58633-58634	-RRB-	denotes	)
T12746	58635-58637	CC	denotes	or
T12747	58638-58646	RB	denotes	directly
T12748	58647-58649	IN	denotes	in
T12749	58650-58657	NNP	denotes	Laemmli
T12750	58658-58665	NN	denotes	bβuffer
T12751	58666-58669	CC	denotes	and
T12752	58670-58676	VBN	denotes	boiled
T12753	58676-58677	.	denotes	.
T12754	58677-58826	sentence	denotes	For skin tissue: Frozen tissue was pulverized in a liquid nitrogen-cooled Gevebesmascher and the powder scraped into a chilled microcentrifuge tube.
T12755	58678-58681	IN	denotes	For
T12757	58682-58686	NN	denotes	skin
T12758	58687-58693	NN	denotes	tissue
T12759	58693-58695	:	denotes	:
T12760	58695-58701	JJ	denotes	Frozen
T12761	58702-58708	NN	denotes	tissue
T12762	58709-58712	VBD	denotes	was
T12756	58713-58723	VBN	denotes	pulverized
T12763	58724-58726	IN	denotes	in
T12764	58727-58728	DT	denotes	a
T12766	58729-58735	JJ	denotes	liquid
T12767	58736-58744	NN	denotes	nitrogen
T12769	58744-58745	HYPH	denotes	-
T12768	58745-58751	VBN	denotes	cooled
T12765	58752-58766	NN	denotes	Gevebesmascher
T12770	58767-58770	CC	denotes	and
T12771	58771-58774	DT	denotes	the
T12772	58775-58781	NN	denotes	powder
T12773	58782-58789	VBN	denotes	scraped
T12774	58790-58794	IN	denotes	into
T12775	58795-58796	DT	denotes	a
T12777	58797-58804	JJ	denotes	chilled
T12778	58805-58820	NN	denotes	microcentrifuge
T12776	58821-58825	NN	denotes	tube
T12779	58825-58826	.	denotes	.
T12780	58826-58983	sentence	denotes	RIPA buffer (1% Triton X-100 in PBS with 10 mM EDTA, 150 mN NaCl, 1% sodium deoxycholate, and 0.1% SDS) and protease inhibitors or Laemmli buffer was added.
T12781	58827-58831	NN	denotes	RIPA
T12782	58832-58838	NN	denotes	buffer
T12784	58839-58840	-LRB-	denotes	(
T12786	58840-58841	CD	denotes	1
T12787	58841-58842	NN	denotes	%
T12788	58843-58849	NN	denotes	Triton
T12785	58850-58851	NN	denotes	X
T12789	58851-58852	HYPH	denotes	-
T12790	58852-58855	CD	denotes	100
T12791	58856-58858	IN	denotes	in
T12792	58859-58862	NN	denotes	PBS
T12793	58863-58867	IN	denotes	with
T12794	58868-58870	CD	denotes	10
T12795	58871-58873	NN	denotes	mM
T12796	58874-58878	NN	denotes	EDTA
T12797	58878-58880	,	denotes	,
T12798	58880-58883	CD	denotes	150
T12799	58884-58886	NN	denotes	mN
T12800	58887-58891	NN	denotes	NaCl
T12801	58891-58893	,	denotes	,
T12802	58893-58894	CD	denotes	1
T12803	58894-58895	NN	denotes	%
T12805	58896-58902	NN	denotes	sodium
T12804	58903-58915	NN	denotes	deoxycholate
T12806	58915-58917	,	denotes	,
T12807	58917-58920	CC	denotes	and
T12808	58921-58924	CD	denotes	0.1
T12809	58924-58925	NN	denotes	%
T12810	58926-58929	NN	denotes	SDS
T12811	58929-58930	-RRB-	denotes	)
T12812	58931-58934	CC	denotes	and
T12813	58935-58943	NN	denotes	protease
T12814	58944-58954	NNS	denotes	inhibitors
T12815	58955-58957	CC	denotes	or
T12816	58958-58965	NNP	denotes	Laemmli
T12817	58966-58972	NN	denotes	buffer
T12818	58973-58976	VBD	denotes	was
T12783	58977-58982	VBN	denotes	added
T12819	58982-58983	.	denotes	.
T12820	58983-59077	sentence	denotes	The cell suspension was sonicated three times for 15 s and centrifuged at 14,000 rpm at 4 °C.
T12821	58984-58987	DT	denotes	The
T12823	58988-58992	NN	denotes	cell
T12822	58993-59003	NN	denotes	suspension
T12825	59004-59007	VBD	denotes	was
T12824	59008-59017	VBN	denotes	sonicated
T12826	59018-59023	CD	denotes	three
T12827	59024-59029	NNS	denotes	times
T12828	59030-59033	IN	denotes	for
T12829	59034-59036	CD	denotes	15
T12830	59037-59038	NN	denotes	s
T12831	59039-59042	CC	denotes	and
T12832	59043-59054	VBN	denotes	centrifuged
T12833	59055-59057	IN	denotes	at
T12834	59058-59064	CD	denotes	14,000
T12835	59065-59068	NN	denotes	rpm
T12836	59069-59071	IN	denotes	at
T12837	59072-59073	CD	denotes	4
T12838	59074-59076	NN	denotes	°C
T12839	59076-59077	.	denotes	.
T12840	59077-59152	sentence	denotes	The supernatant was separated from the pellet and used in the experiments.
T12841	59078-59081	DT	denotes	The
T12842	59082-59093	NN	denotes	supernatant
T12844	59094-59097	VBD	denotes	was
T12843	59098-59107	VBN	denotes	separated
T12845	59108-59112	IN	denotes	from
T12846	59113-59116	DT	denotes	the
T12847	59117-59123	NN	denotes	pellet
T12848	59124-59127	CC	denotes	and
T12849	59128-59132	VBN	denotes	used
T12850	59133-59135	IN	denotes	in
T12851	59136-59139	DT	denotes	the
T12852	59140-59151	NNS	denotes	experiments
T12853	59151-59152	.	denotes	.
T12854	59152-59343	sentence	denotes	Extracts subjected to immunoprecipitation were precleared with Protein G Sepharose (Amersham, Piscataway, New York, United States) and incubated with antibody with rocking overnight at 4 °C.
T12855	59153-59161	NNS	denotes	Extracts
T12857	59162-59171	VBN	denotes	subjected
T12858	59172-59174	IN	denotes	to
T12859	59175-59194	NN	denotes	immunoprecipitation
T12860	59195-59199	VBD	denotes	were
T12856	59200-59210	VBN	denotes	precleared
T12861	59211-59215	IN	denotes	with
T12862	59216-59223	NN	denotes	Protein
T12863	59224-59225	NN	denotes	G
T12864	59226-59235	NN	denotes	Sepharose
T12865	59236-59237	-LRB-	denotes	(
T12866	59237-59245	NNP	denotes	Amersham
T12867	59245-59247	,	denotes	,
T12868	59247-59257	NNP	denotes	Piscataway
T12869	59257-59259	,	denotes	,
T12870	59259-59262	NNP	denotes	New
T12871	59263-59267	NNP	denotes	York
T12872	59267-59269	,	denotes	,
T12873	59269-59275	NNP	denotes	United
T12874	59276-59282	NNP	denotes	States
T12875	59282-59283	-RRB-	denotes	)
T12876	59284-59287	CC	denotes	and
T12877	59288-59297	VBN	denotes	incubated
T12878	59298-59302	IN	denotes	with
T12879	59303-59311	NN	denotes	antibody
T12880	59312-59316	IN	denotes	with
T12881	59317-59324	NN	denotes	rocking
T12882	59325-59334	RB	denotes	overnight
T12883	59335-59337	IN	denotes	at
T12884	59338-59339	CD	denotes	4
T12885	59340-59342	NN	denotes	°C
T12886	59342-59343	.	denotes	.
T12887	59343-59430	sentence	denotes	Protein G Sepharose was added and samples were incubated for 1 h at 4 °C with rocking.
T12888	59344-59351	NN	denotes	Protein
T12889	59352-59353	NN	denotes	G
T12890	59354-59363	NN	denotes	Sepharose
T12892	59364-59367	VBD	denotes	was
T12891	59368-59373	VBN	denotes	added
T12893	59374-59377	CC	denotes	and
T12894	59378-59385	NNS	denotes	samples
T12896	59386-59390	VBD	denotes	were
T12895	59391-59400	VBN	denotes	incubated
T12897	59401-59404	IN	denotes	for
T12898	59405-59406	CD	denotes	1
T12899	59407-59408	NN	denotes	h
T12900	59409-59411	IN	denotes	at
T12901	59412-59413	CD	denotes	4
T12902	59414-59416	NN	denotes	°C
T12903	59417-59421	IN	denotes	with
T12904	59422-59429	NN	denotes	rocking
T12905	59429-59430	.	denotes	.
T12906	59430-59603	sentence	denotes	Samples were washed three times for 5 min each in lysis buffer, and the Protein G Sepharose-antibody-antigen pellet was resuspended in Laemmli buffer and boiled for 10 min.
T12907	59431-59438	NNS	denotes	Samples
T12909	59439-59443	VBD	denotes	were
T12908	59444-59450	VBN	denotes	washed
T12910	59451-59456	CD	denotes	three
T12911	59457-59462	NNS	denotes	times
T12912	59463-59466	IN	denotes	for
T12913	59467-59468	CD	denotes	5
T12914	59469-59472	NN	denotes	min
T12915	59473-59477	RB	denotes	each
T12916	59478-59480	IN	denotes	in
T12917	59481-59486	NN	denotes	lysis
T12918	59487-59493	NN	denotes	buffer
T12919	59493-59495	,	denotes	,
T12920	59495-59498	CC	denotes	and
T12921	59499-59502	DT	denotes	the
T12923	59503-59510	NN	denotes	Protein
T12924	59511-59512	NN	denotes	G
T12926	59513-59522	NN	denotes	Sepharose
T12927	59522-59523	HYPH	denotes	-
T12928	59523-59531	NN	denotes	antibody
T12929	59531-59532	HYPH	denotes	-
T12925	59532-59539	NN	denotes	antigen
T12922	59540-59546	NN	denotes	pellet
T12931	59547-59550	VBD	denotes	was
T12930	59551-59562	VBN	denotes	resuspended
T12932	59563-59565	IN	denotes	in
T12933	59566-59573	NNP	denotes	Laemmli
T12934	59574-59580	NN	denotes	buffer
T12935	59581-59584	CC	denotes	and
T12936	59585-59591	VBN	denotes	boiled
T12937	59592-59595	IN	denotes	for
T12938	59596-59598	CD	denotes	10
T12939	59599-59602	NN	denotes	min
T12940	59602-59603	.	denotes	.
T12941	59603-59749	sentence	denotes	Samples were run on SDS-PAGE and transferred to nitrocellulose membrane (Schleicher and Schuell Bioscience, Keene, New Hampshire, United States).
T12942	59604-59611	NNS	denotes	Samples
T12944	59612-59616	VBD	denotes	were
T12943	59617-59620	VBN	denotes	run
T12945	59621-59623	IN	denotes	on
T12946	59624-59627	NN	denotes	SDS
T12948	59627-59628	HYPH	denotes	-
T12947	59628-59632	NN	denotes	PAGE
T12949	59633-59636	CC	denotes	and
T12950	59637-59648	VBN	denotes	transferred
T12951	59649-59651	IN	denotes	to
T12952	59652-59666	NN	denotes	nitrocellulose
T12953	59667-59675	NN	denotes	membrane
T12954	59676-59677	-LRB-	denotes	(
T12956	59677-59687	NNP	denotes	Schleicher
T12957	59688-59691	CC	denotes	and
T12958	59692-59699	NNP	denotes	Schuell
T12955	59700-59710	NNP	denotes	Bioscience
T12959	59710-59712	,	denotes	,
T12960	59712-59717	NNP	denotes	Keene
T12961	59717-59719	,	denotes	,
T12962	59719-59722	NNP	denotes	New
T12963	59723-59732	NNP	denotes	Hampshire
T12964	59732-59734	,	denotes	,
T12965	59734-59740	NNP	denotes	United
T12966	59741-59747	NNP	denotes	States
T12967	59747-59748	-RRB-	denotes	)
T12968	59748-59749	.	denotes	.
T12969	59749-59840	sentence	denotes	Western blot signals were developed using the enhanced chemiluminescence kit from Amersham
T12970	59750-59757	NNP	denotes	Western
T12971	59758-59762	NN	denotes	blot
T12972	59763-59770	NNS	denotes	signals
T12974	59771-59775	VBD	denotes	were
T12973	59776-59785	VBN	denotes	developed
T12975	59786-59791	VBG	denotes	using
T12976	59792-59795	DT	denotes	the
T12978	59796-59804	VBN	denotes	enhanced
T12979	59805-59822	NN	denotes	chemiluminescence
T12977	59823-59826	NN	denotes	kit
T12980	59827-59831	IN	denotes	from
T12981	59832-59840	NNP	denotes	Amersham
T13041	59842-59846	NN	denotes	Cell
T13042	59847-59854	NN	denotes	culture
T13043	59854-59940	sentence	denotes	Primary keratinocytes were culture in low-calcium medium as previously described [4].
T13044	59855-59862	JJ	denotes	Primary
T13045	59863-59876	NNS	denotes	keratinocytes
T13047	59877-59881	VBD	denotes	were
T13046	59882-59889	VBN	denotes	culture
T13048	59890-59892	IN	denotes	in
T13049	59893-59896	JJ	denotes	low
T13051	59896-59897	HYPH	denotes	-
T13050	59897-59904	NN	denotes	calcium
T13052	59905-59911	NN	denotes	medium
T13053	59912-59914	IN	denotes	as
T13055	59915-59925	RB	denotes	previously
T13054	59926-59935	VBN	denotes	described
T13056	59936-59937	-LRB-	denotes	[
T13057	59937-59938	CD	denotes	4
T13058	59938-59939	-RRB-	denotes	]
T13059	59939-59940	.	denotes	.
T13060	59940-60090	sentence	denotes	Transient transfections were carried out with FuGENE6 reagent (Roche, Indianapolis, Indiana, United States) according to the manufacturer's protocol.
T13061	59941-59950	JJ	denotes	Transient
T13062	59951-59964	NNS	denotes	transfections
T13064	59965-59969	VBD	denotes	were
T13063	59970-59977	VBN	denotes	carried
T13065	59978-59981	RP	denotes	out
T13066	59982-59986	IN	denotes	with
T13067	59987-59994	NN	denotes	FuGENE6
T13068	59995-60002	NN	denotes	reagent
T13069	60003-60004	-LRB-	denotes	(
T13070	60004-60009	NNP	denotes	Roche
T13071	60009-60011	,	denotes	,
T13072	60011-60023	NNP	denotes	Indianapolis
T13073	60023-60025	,	denotes	,
T13074	60025-60032	NNP	denotes	Indiana
T13075	60032-60034	,	denotes	,
T13076	60034-60040	NNP	denotes	United
T13077	60041-60047	NNP	denotes	States
T13078	60047-60048	-RRB-	denotes	)
T13079	60049-60058	VBG	denotes	according
T13080	60059-60061	IN	denotes	to
T13081	60062-60065	DT	denotes	the
T13082	60066-60078	NN	denotes	manufacturer
T13084	60078-60080	POS	denotes	's
T13083	60081-60089	NN	denotes	protocol
T13085	60089-60090	.	denotes	.
T13086	60090-60352	sentence	denotes	Measurement of β-galactosidase or luciferase levels in promoter activity studies were carried out with the Galacto-Lite assay kit (TROPIX, Bedford, Massachusetts, United States) and the Dual luciferase (Promega, Madison, Wisconsin, United States), respectively.
T13087	60091-60102	NN	denotes	Measurement
T13089	60103-60105	IN	denotes	of
T13090	60106-60107	NN	denotes	β
T13092	60107-60108	HYPH	denotes	-
T13091	60108-60121	NN	denotes	galactosidase
T13094	60122-60124	CC	denotes	or
T13095	60125-60135	NN	denotes	luciferase
T13093	60136-60142	NNS	denotes	levels
T13096	60143-60145	IN	denotes	in
T13097	60146-60154	NN	denotes	promoter
T13098	60155-60163	NN	denotes	activity
T13099	60164-60171	NNS	denotes	studies
T13100	60172-60176	VBD	denotes	were
T13088	60177-60184	VBN	denotes	carried
T13101	60185-60188	RP	denotes	out
T13102	60189-60193	IN	denotes	with
T13103	60194-60197	DT	denotes	the
T13105	60198-60205	NNP	denotes	Galacto
T13107	60205-60206	HYPH	denotes	-
T13106	60206-60210	NNP	denotes	Lite
T13108	60211-60216	NN	denotes	assay
T13104	60217-60220	NN	denotes	kit
T13109	60221-60222	-LRB-	denotes	(
T13110	60222-60228	NNP	denotes	TROPIX
T13111	60228-60230	,	denotes	,
T13112	60230-60237	NNP	denotes	Bedford
T13113	60237-60239	,	denotes	,
T13114	60239-60252	NNP	denotes	Massachusetts
T13115	60252-60254	,	denotes	,
T13116	60254-60260	NNP	denotes	United
T13117	60261-60267	NNP	denotes	States
T13118	60267-60268	-RRB-	denotes	)
T13119	60269-60272	CC	denotes	and
T13120	60273-60276	DT	denotes	the
T13122	60277-60281	JJ	denotes	Dual
T13121	60282-60292	NN	denotes	luciferase
T13123	60293-60294	-LRB-	denotes	(
T13124	60294-60301	NNP	denotes	Promega
T13125	60301-60303	,	denotes	,
T13126	60303-60310	NNP	denotes	Madison
T13127	60310-60312	,	denotes	,
T13128	60312-60321	NNP	denotes	Wisconsin
T13129	60321-60323	,	denotes	,
T13130	60323-60329	NNP	denotes	United
T13131	60330-60336	NNP	denotes	States
T13132	60336-60337	-RRB-	denotes	)
T13133	60337-60339	,	denotes	,
T13134	60339-60351	RB	denotes	respectively
T13135	60351-60352	.	denotes	.
T13136	60352-60440	sentence	denotes	Runella luciferase was cotransfected into cells to correct for transfection efficiency.
T13137	60353-60360	NN	denotes	Runella
T13138	60361-60371	NN	denotes	luciferase
T13140	60372-60375	VBD	denotes	was
T13139	60376-60389	VBN	denotes	cotransfected
T13141	60390-60394	IN	denotes	into
T13142	60395-60400	NNS	denotes	cells
T13143	60401-60403	TO	denotes	to
T13144	60404-60411	VB	denotes	correct
T13145	60412-60415	IN	denotes	for
T13146	60416-60428	NN	denotes	transfection
T13147	60429-60439	NN	denotes	efficiency
T13148	60439-60440	.	denotes	.
T13149	60440-60511	sentence	denotes	Experiments were done in triplicate and repeated at least three times.
T13150	60441-60452	NNS	denotes	Experiments
T13152	60453-60457	VBD	denotes	were
T13151	60458-60462	VBN	denotes	done
T13153	60463-60465	IN	denotes	in
T13154	60466-60476	NN	denotes	triplicate
T13155	60477-60480	CC	denotes	and
T13156	60481-60489	VBN	denotes	repeated
T13157	60490-60492	RB	denotes	at
T13159	60493-60498	RBS	denotes	least
T13158	60499-60504	CD	denotes	three
T13160	60505-60510	NNS	denotes	times
T13161	60510-60511	.	denotes	.
T13162	60511-60606	sentence	denotes	Measurements were done on a luminometer (MGM Instruments, Hamden, Connecticut, United States).
T13163	60512-60524	NNS	denotes	Measurements
T13165	60525-60529	VBD	denotes	were
T13164	60530-60534	VBN	denotes	done
T13166	60535-60537	IN	denotes	on
T13167	60538-60539	DT	denotes	a
T13168	60540-60551	NN	denotes	luminometer
T13169	60552-60553	-LRB-	denotes	(
T13171	60553-60556	NNP	denotes	MGM
T13170	60557-60568	NNP	denotes	Instruments
T13172	60568-60570	,	denotes	,
T13173	60570-60576	NNP	denotes	Hamden
T13174	60576-60578	,	denotes	,
T13175	60578-60589	NNP	denotes	Connecticut
T13176	60589-60591	,	denotes	,
T13177	60591-60597	NNP	denotes	United
T13178	60598-60604	NNP	denotes	States
T13179	60604-60605	-RRB-	denotes	)
T13180	60605-60606	.	denotes	.
T13181	60606-60766	sentence	denotes	For experiments measuring phosphorylation of MAPK, keratinocytes were serum starved for 3 h prior to harvesting of cells by incubation in medium lacking serum.
T13182	60607-60610	IN	denotes	For
T13184	60611-60622	NNS	denotes	experiments
T13185	60623-60632	VBG	denotes	measuring
T13186	60633-60648	NN	denotes	phosphorylation
T13187	60649-60651	IN	denotes	of
T13188	60652-60656	NN	denotes	MAPK
T13189	60656-60658	,	denotes	,
T13190	60658-60671	NNS	denotes	keratinocytes
T13191	60672-60676	VBD	denotes	were
T13192	60677-60682	NN	denotes	serum
T13183	60683-60690	VBN	denotes	starved
T13193	60691-60694	IN	denotes	for
T13194	60695-60696	CD	denotes	3
T13195	60697-60698	NN	denotes	h
T13196	60699-60704	JJ	denotes	prior
T13197	60705-60707	IN	denotes	to
T13198	60708-60718	NN	denotes	harvesting
T13199	60719-60721	IN	denotes	of
T13200	60722-60727	NNS	denotes	cells
T13201	60728-60730	IN	denotes	by
T13202	60731-60741	NN	denotes	incubation
T13203	60742-60744	IN	denotes	in
T13204	60745-60751	NN	denotes	medium
T13205	60752-60759	VBG	denotes	lacking
T13206	60760-60765	NN	denotes	serum
T13207	60765-60766	.	denotes	.
T13208	60766-60855	sentence	denotes	Treatment of cells with Wnt- and noggin-conditioned medium was previously described [4].
T13209	60767-60776	NN	denotes	Treatment
T13211	60777-60779	IN	denotes	of
T13212	60780-60785	NNS	denotes	cells
T13213	60786-60790	IN	denotes	with
T13214	60791-60794	NN	denotes	Wnt
T13216	60794-60795	HYPH	denotes	-
T13217	60796-60799	CC	denotes	and
T13218	60800-60806	NN	denotes	noggin
T13219	60806-60807	HYPH	denotes	-
T13215	60807-60818	VBN	denotes	conditioned
T13220	60819-60825	NN	denotes	medium
T13221	60826-60829	VBD	denotes	was
T13222	60830-60840	RB	denotes	previously
T13210	60841-60850	VBN	denotes	described
T13223	60851-60852	-LRB-	denotes	[
T13224	60852-60853	CD	denotes	4
T13225	60853-60854	-RRB-	denotes	]
T13226	60854-60855	.	denotes	.
T13308	60857-60867	NNS	denotes	Constructs
T13309	60867-61148	sentence	denotes	The 2.2-kb murine Snail promoter was generated by PCR using a forward primer with an XbaI linker sequence, 5′- TCTAGAATTGTTTGCTGCTGTATGGTCTTC-3′, along with a reverse primer with a BglII linker sequence, 5′- AGATCTGTTGGCCAGAGCGACCTAG- GTAG-3′, and mouse genomic DNA as a template.
T13310	60868-60871	DT	denotes	The
T13312	60872-60875	CD	denotes	2.2
T13314	60875-60876	HYPH	denotes	-
T13313	60876-60878	NN	denotes	kb
T13315	60879-60885	JJ	denotes	murine
T13316	60886-60891	NN	denotes	Snail
T13311	60892-60900	NN	denotes	promoter
T13318	60901-60904	VBD	denotes	was
T13317	60905-60914	VBN	denotes	generated
T13319	60915-60917	IN	denotes	by
T13320	60918-60921	NN	denotes	PCR
T13321	60922-60927	VBG	denotes	using
T13322	60928-60929	DT	denotes	a
T13324	60930-60937	JJ	denotes	forward
T13323	60938-60944	NN	denotes	primer
T13325	60945-60949	IN	denotes	with
T13326	60950-60952	DT	denotes	an
T13328	60953-60957	NN	denotes	XbaI
T13329	60958-60964	NN	denotes	linker
T13327	60965-60973	NN	denotes	sequence
T13330	60973-60975	,	denotes	,
T13331	60975-60976	CD	denotes	5
T13333	60976-60977	SYM	denotes	′
T13334	60977-60978	HYPH	denotes	-
T13332	60979-61009	NN	denotes	TCTAGAATTGTTTGCTGCTGTATGGTCTTC
T13335	61009-61010	HYPH	denotes	-
T13336	61010-61011	CD	denotes	3
T13337	61011-61012	SYM	denotes	′
T13338	61012-61014	,	denotes	,
T13339	61014-61019	IN	denotes	along
T13340	61020-61024	IN	denotes	with
T13341	61025-61026	DT	denotes	a
T13343	61027-61034	JJ	denotes	reverse
T13342	61035-61041	NN	denotes	primer
T13344	61042-61046	IN	denotes	with
T13345	61047-61048	DT	denotes	a
T13347	61049-61054	NN	denotes	BglII
T13348	61055-61061	NN	denotes	linker
T13346	61062-61070	NN	denotes	sequence
T13349	61070-61072	,	denotes	,
T13350	61072-61073	CD	denotes	5
T13352	61073-61074	SYM	denotes	′
T13353	61074-61075	HYPH	denotes	-
T13354	61076-61101	NN	denotes	AGATCTGTTGGCCAGAGCGACCTAG
T13355	61101-61102	HYPH	denotes	-
T13351	61103-61107	NN	denotes	GTAG
T13356	61107-61108	HYPH	denotes	-
T13357	61108-61109	CD	denotes	3
T13358	61109-61110	SYM	denotes	′
T13359	61110-61112	,	denotes	,
T13360	61112-61115	CC	denotes	and
T13361	61116-61121	NN	denotes	mouse
T13363	61122-61129	JJ	denotes	genomic
T13362	61130-61133	NN	denotes	DNA
T13364	61134-61136	IN	denotes	as
T13365	61137-61138	DT	denotes	a
T13366	61139-61147	NN	denotes	template
T13367	61147-61148	.	denotes	.
T13368	61148-61340	sentence	denotes	The PCR product was purified with the Gel Extraction Kit (Qiagen, Valencia, California, United States) and ligated into pCRII-TOPO TA vector (Invitrogen, Carlsbad, California, United States).
T13369	61149-61152	DT	denotes	The
T13371	61153-61156	NN	denotes	PCR
T13370	61157-61164	NN	denotes	product
T13373	61165-61168	VBD	denotes	was
T13372	61169-61177	VBN	denotes	purified
T13374	61178-61182	IN	denotes	with
T13375	61183-61186	DT	denotes	the
T13377	61187-61190	NN	denotes	Gel
T13378	61191-61201	NN	denotes	Extraction
T13376	61202-61205	NN	denotes	Kit
T13379	61206-61207	-LRB-	denotes	(
T13380	61207-61213	NNP	denotes	Qiagen
T13381	61213-61215	,	denotes	,
T13382	61215-61223	NNP	denotes	Valencia
T13383	61223-61225	,	denotes	,
T13384	61225-61235	NNP	denotes	California
T13385	61235-61237	,	denotes	,
T13386	61237-61243	NNP	denotes	United
T13387	61244-61250	NNP	denotes	States
T13388	61250-61251	-RRB-	denotes	)
T13389	61252-61255	CC	denotes	and
T13390	61256-61263	VBN	denotes	ligated
T13391	61264-61268	IN	denotes	into
T13392	61269-61274	NN	denotes	pCRII
T13394	61274-61275	HYPH	denotes	-
T13393	61275-61279	NN	denotes	TOPO
T13396	61280-61282	NN	denotes	TA
T13395	61283-61289	NN	denotes	vector
T13397	61290-61291	-LRB-	denotes	(
T13398	61291-61301	NNP	denotes	Invitrogen
T13399	61301-61303	,	denotes	,
T13400	61303-61311	NNP	denotes	Carlsbad
T13401	61311-61313	,	denotes	,
T13402	61313-61323	NNP	denotes	California
T13403	61323-61325	,	denotes	,
T13404	61325-61331	NNP	denotes	United
T13405	61332-61338	NNP	denotes	States
T13406	61338-61339	-RRB-	denotes	)
T13407	61339-61340	.	denotes	.
T13408	61340-61521	sentence	denotes	The promoter was verified by sequencing and digested with XbaI and BglII and subcloned into the pβ-gal BASIC vector (BD Biosciences Clontech, Palo Alto, California, United States).
T13409	61341-61344	DT	denotes	The
T13410	61345-61353	NN	denotes	promoter
T13412	61354-61357	VBD	denotes	was
T13411	61358-61366	VBN	denotes	verified
T13413	61367-61369	IN	denotes	by
T13414	61370-61380	NN	denotes	sequencing
T13415	61381-61384	CC	denotes	and
T13416	61385-61393	VBN	denotes	digested
T13417	61394-61398	IN	denotes	with
T13418	61399-61403	NN	denotes	XbaI
T13419	61404-61407	CC	denotes	and
T13420	61408-61413	NN	denotes	BglII
T13421	61414-61417	CC	denotes	and
T13422	61418-61427	VBN	denotes	subcloned
T13423	61428-61432	IN	denotes	into
T13424	61433-61436	DT	denotes	the
T13426	61437-61439	NN	denotes	pβ
T13428	61439-61440	HYPH	denotes	-
T13427	61440-61443	NN	denotes	gal
T13429	61444-61449	NN	denotes	BASIC
T13425	61450-61456	NN	denotes	vector
T13430	61457-61458	-LRB-	denotes	(
T13432	61458-61460	NNP	denotes	BD
T13433	61461-61472	NNP	denotes	Biosciences
T13431	61473-61481	NNP	denotes	Clontech
T13434	61481-61483	,	denotes	,
T13435	61483-61487	NNP	denotes	Palo
T13436	61488-61492	NNP	denotes	Alto
T13437	61492-61494	,	denotes	,
T13438	61494-61504	NNP	denotes	California
T13439	61504-61506	,	denotes	,
T13440	61506-61512	NNP	denotes	United
T13441	61513-61519	NNP	denotes	States
T13442	61519-61520	-RRB-	denotes	)
T13443	61520-61521	.	denotes	.
T13444	61521-61815	sentence	denotes	The point mutations in the SMAD binding element was generated with the Quik-Change Kit (Stratagene, La Jolla, California, United States) using the forward primer 5′- GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC-3′ and the reverse primer 5′- GCTTTT- CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC-3′.
T13445	61522-61525	DT	denotes	The
T13447	61526-61531	NN	denotes	point
T13446	61532-61541	NNS	denotes	mutations
T13449	61542-61544	IN	denotes	in
T13450	61545-61548	DT	denotes	the
T13452	61549-61553	NN	denotes	SMAD
T13453	61554-61561	NN	denotes	binding
T13451	61562-61569	NN	denotes	element
T13454	61570-61573	VBD	denotes	was
T13448	61574-61583	VBN	denotes	generated
T13455	61584-61588	IN	denotes	with
T13456	61589-61592	DT	denotes	the
T13458	61593-61597	NNP	denotes	Quik
T13460	61597-61598	HYPH	denotes	-
T13459	61598-61604	NNP	denotes	Change
T13457	61605-61608	NNP	denotes	Kit
T13461	61609-61610	-LRB-	denotes	(
T13462	61610-61620	NNP	denotes	Stratagene
T13463	61620-61622	,	denotes	,
T13464	61622-61624	NNP	denotes	La
T13465	61625-61630	NNP	denotes	Jolla
T13466	61630-61632	,	denotes	,
T13467	61632-61642	NNP	denotes	California
T13468	61642-61644	,	denotes	,
T13469	61644-61650	NNP	denotes	United
T13470	61651-61657	NNP	denotes	States
T13471	61657-61658	-RRB-	denotes	)
T13472	61659-61664	VBG	denotes	using
T13473	61665-61668	DT	denotes	the
T13475	61669-61676	JJ	denotes	forward
T13474	61677-61683	NN	denotes	primer
T13476	61684-61685	CD	denotes	5
T13478	61685-61686	SYM	denotes	′
T13479	61686-61687	HYPH	denotes	-
T13477	61688-61733	NN	denotes	GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
T13480	61733-61734	HYPH	denotes	-
T13481	61734-61735	CD	denotes	3
T13482	61735-61736	SYM	denotes	′
T13483	61737-61740	CC	denotes	and
T13484	61741-61744	DT	denotes	the
T13486	61745-61752	JJ	denotes	reverse
T13485	61753-61759	NN	denotes	primer
T13487	61760-61761	CD	denotes	5
T13489	61761-61762	SYM	denotes	′
T13490	61762-61763	HYPH	denotes	-
T13491	61764-61770	NN	denotes	GCTTTT
T13492	61770-61771	HYPH	denotes	-
T13488	61772-61811	NN	denotes	CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
T13493	61811-61812	HYPH	denotes	-
T13494	61812-61813	CD	denotes	3
T13495	61813-61814	SYM	denotes	′
T13496	61814-61815	.	denotes	.
T13497	61815-62048	sentence	denotes	The probes for the Snail in situ hybridization were generated against the 3′ UTR by PCR using the forward primer 5′- ACCTTCTCCCGCATGTCCTTGCTCC-3′ and the reverse primer 5′- CTGCTGAGGCATGGTTACAGCTGG-3′, and genomic DNA as a template.
T13498	61816-61819	DT	denotes	The
T13499	61820-61826	NNS	denotes	probes
T13501	61827-61830	IN	denotes	for
T13502	61831-61834	DT	denotes	the
T13504	61835-61840	NN	denotes	Snail
T13505	61841-61843	FW	denotes	in
T13506	61844-61848	FW	denotes	situ
T13503	61849-61862	NN	denotes	hybridization
T13507	61863-61867	VBD	denotes	were
T13500	61868-61877	VBN	denotes	generated
T13508	61878-61885	IN	denotes	against
T13509	61886-61889	DT	denotes	the
T13511	61890-61891	CD	denotes	3
T13512	61891-61892	SYM	denotes	′
T13510	61893-61896	NN	denotes	UTR
T13513	61897-61899	IN	denotes	by
T13514	61900-61903	NN	denotes	PCR
T13515	61904-61909	VBG	denotes	using
T13516	61910-61913	DT	denotes	the
T13518	61914-61921	JJ	denotes	forward
T13517	61922-61928	NN	denotes	primer
T13519	61929-61930	CD	denotes	5
T13521	61930-61931	SYM	denotes	′
T13522	61931-61932	HYPH	denotes	-
T13520	61933-61958	NN	denotes	ACCTTCTCCCGCATGTCCTTGCTCC
T13523	61958-61959	HYPH	denotes	-
T13524	61959-61960	CD	denotes	3
T13525	61960-61961	SYM	denotes	′
T13526	61962-61965	CC	denotes	and
T13527	61966-61969	DT	denotes	the
T13529	61970-61977	JJ	denotes	reverse
T13528	61978-61984	NN	denotes	primer
T13530	61985-61986	CD	denotes	5
T13532	61986-61987	SYM	denotes	′
T13533	61987-61988	HYPH	denotes	-
T13531	61989-62013	NN	denotes	CTGCTGAGGCATGGTTACAGCTGG
T13534	62013-62014	HYPH	denotes	-
T13535	62014-62015	CD	denotes	3
T13536	62015-62016	SYM	denotes	′
T13537	62016-62018	,	denotes	,
T13538	62018-62021	CC	denotes	and
T13539	62022-62029	JJ	denotes	genomic
T13540	62030-62033	NN	denotes	DNA
T13541	62034-62036	IN	denotes	as
T13542	62037-62038	DT	denotes	a
T13543	62039-62047	NN	denotes	template
T13544	62047-62048	.	denotes	.
T13545	62048-62120	sentence	denotes	The PCR product was gel purified and ligated into pCRII-TOPO TA vector.
T13546	62049-62052	DT	denotes	The
T13548	62053-62056	NN	denotes	PCR
T13547	62057-62064	NN	denotes	product
T13550	62065-62068	VBD	denotes	was
T13551	62069-62072	NN	denotes	gel
T13549	62073-62081	VBN	denotes	purified
T13552	62082-62085	CC	denotes	and
T13553	62086-62093	VBN	denotes	ligated
T13554	62094-62098	IN	denotes	into
T13555	62099-62104	NN	denotes	pCRII
T13557	62104-62105	HYPH	denotes	-
T13556	62105-62109	NN	denotes	TOPO
T13559	62110-62112	NN	denotes	TA
T13558	62113-62119	NN	denotes	vector
T13560	62119-62120	.	denotes	.
T13561	62120-62281	sentence	denotes	The pre-LIM domain of Ajuba was generated essentially as described [9], but was fused to GFP by subcloning from the pEGFP-N1 20 vector (BD Biosciences Clontech)
T13562	62121-62124	DT	denotes	The
T13564	62125-62132	JJ	denotes	pre-LIM
T13563	62133-62139	NN	denotes	domain
T13566	62140-62142	IN	denotes	of
T13567	62143-62148	NN	denotes	Ajuba
T13568	62149-62152	VBD	denotes	was
T13565	62153-62162	VBN	denotes	generated
T13569	62163-62174	RB	denotes	essentially
T13570	62175-62177	IN	denotes	as
T13571	62178-62187	VBN	denotes	described
T13572	62188-62189	-LRB-	denotes	[
T13573	62189-62190	CD	denotes	9
T13574	62190-62191	-RRB-	denotes	]
T13575	62191-62193	,	denotes	,
T13576	62193-62196	CC	denotes	but
T13577	62197-62200	VBD	denotes	was
T13578	62201-62206	VBN	denotes	fused
T13579	62207-62209	IN	denotes	to
T13580	62210-62213	NN	denotes	GFP
T13581	62214-62216	IN	denotes	by
T13582	62217-62227	VBG	denotes	subcloning
T13583	62228-62232	IN	denotes	from
T13584	62233-62236	DT	denotes	the
T13586	62237-62242	NN	denotes	pEGFP
T13588	62242-62243	HYPH	denotes	-
T13587	62243-62245	NN	denotes	N1
T13589	62246-62248	CD	denotes	20
T13585	62249-62255	NN	denotes	vector
T13590	62256-62257	-LRB-	denotes	(
T13592	62257-62259	NNP	denotes	BD
T13593	62260-62271	NNP	denotes	Biosciences
T13591	62272-62280	NNP	denotes	Clontech
T13594	62280-62281	-RRB-	denotes	)
T13649	62283-62285	FW	denotes	In
T13650	62286-62290	FW	denotes	situ
T13651	62291-62304	NN	denotes	hybridization
T13652	62304-62467	sentence	denotes	The pCRII-TOPO TA vector containing a region of the 3′ UTR of Snail was used as a template to generate digoxigenin-labeled sense and antisense riboprobes (Roche).
T13653	62305-62308	DT	denotes	The
T13655	62309-62314	NN	denotes	pCRII
T13657	62314-62315	HYPH	denotes	-
T13656	62315-62319	NN	denotes	TOPO
T13658	62320-62322	NN	denotes	TA
T13654	62323-62329	NN	denotes	vector
T13660	62330-62340	VBG	denotes	containing
T13661	62341-62342	DT	denotes	a
T13662	62343-62349	NN	denotes	region
T13663	62350-62352	IN	denotes	of
T13664	62353-62356	DT	denotes	the
T13666	62357-62358	CD	denotes	3
T13667	62358-62359	SYM	denotes	′
T13665	62360-62363	NN	denotes	UTR
T13668	62364-62366	IN	denotes	of
T13669	62367-62372	NN	denotes	Snail
T13670	62373-62376	VBD	denotes	was
T13659	62377-62381	VBN	denotes	used
T13671	62382-62384	IN	denotes	as
T13672	62385-62386	DT	denotes	a
T13673	62387-62395	NN	denotes	template
T13674	62396-62398	TO	denotes	to
T13675	62399-62407	VB	denotes	generate
T13676	62408-62419	NN	denotes	digoxigenin
T13678	62419-62420	HYPH	denotes	-
T13677	62420-62427	VBN	denotes	labeled
T13680	62428-62433	NN	denotes	sense
T13681	62434-62437	CC	denotes	and
T13682	62438-62447	NN	denotes	antisense
T13679	62448-62458	NNS	denotes	riboprobes
T13683	62459-62460	-LRB-	denotes	(
T13684	62460-62465	NNP	denotes	Roche
T13685	62465-62466	-RRB-	denotes	)
T13686	62466-62467	.	denotes	.
T13687	62467-62533	sentence	denotes	The respective probes were obtained by XhoI and BamH1 digestions.
T13688	62468-62471	DT	denotes	The
T13690	62472-62482	JJ	denotes	respective
T13689	62483-62489	NNS	denotes	probes
T13692	62490-62494	VBD	denotes	were
T13691	62495-62503	VBN	denotes	obtained
T13693	62504-62506	IN	denotes	by
T13694	62507-62511	NN	denotes	XhoI
T13696	62512-62515	CC	denotes	and
T13697	62516-62521	NN	denotes	BamH1
T13695	62522-62532	NNS	denotes	digestions
T13698	62532-62533	.	denotes	.
T13699	62533-62619	sentence	denotes	In situ hybridizations were performed on 10-μm thick sections of E17.5 mouse embryos.
T13700	62534-62536	FW	denotes	In
T13701	62537-62541	FW	denotes	situ
T13702	62542-62556	NNS	denotes	hybridizations
T13704	62557-62561	VBD	denotes	were
T13703	62562-62571	VBN	denotes	performed
T13705	62572-62574	IN	denotes	on
T13706	62575-62577	CD	denotes	10
T13708	62577-62578	HYPH	denotes	-
T13707	62578-62580	NN	denotes	μm
T13709	62581-62586	JJ	denotes	thick
T13710	62587-62595	NNS	denotes	sections
T13711	62596-62598	IN	denotes	of
T13712	62599-62604	NN	denotes	E17.5
T13714	62605-62610	NN	denotes	mouse
T13713	62611-62618	NNS	denotes	embryos
T13715	62618-62619	.	denotes	.
T13716	62619-62902	sentence	denotes	The sections were fixed with 4% PFA for 10 min at room temperature, prehybridized at room temperature for 4.5 h, hybridized with the probe (2 μg/ml) at 55 °C for 12–14 h, blocked with 10% NGS, and treated with anti-dig Fab-AP antibody (Roche #1093274) at a 1:2,500 dilution for 3 h.
T13717	62620-62623	DT	denotes	The
T13718	62624-62632	NNS	denotes	sections
T13720	62633-62637	VBD	denotes	were
T13719	62638-62643	VBN	denotes	fixed
T13721	62644-62648	IN	denotes	with
T13722	62649-62650	CD	denotes	4
T13723	62650-62651	NN	denotes	%
T13724	62652-62655	NN	denotes	PFA
T13725	62656-62659	IN	denotes	for
T13726	62660-62662	CD	denotes	10
T13727	62663-62666	NN	denotes	min
T13728	62667-62669	IN	denotes	at
T13729	62670-62674	NN	denotes	room
T13730	62675-62686	NN	denotes	temperature
T13731	62686-62688	,	denotes	,
T13732	62688-62701	VBN	denotes	prehybridized
T13733	62702-62704	IN	denotes	at
T13734	62705-62709	NN	denotes	room
T13735	62710-62721	NN	denotes	temperature
T13736	62722-62725	IN	denotes	for
T13737	62726-62729	CD	denotes	4.5
T13738	62730-62731	NN	denotes	h
T13739	62731-62733	,	denotes	,
T13740	62733-62743	VBN	denotes	hybridized
T13741	62744-62748	IN	denotes	with
T13742	62749-62752	DT	denotes	the
T13743	62753-62758	NN	denotes	probe
T13744	62759-62760	-LRB-	denotes	(
T13746	62760-62761	CD	denotes	2
T13745	62762-62764	NN	denotes	μg
T13747	62764-62765	SYM	denotes	/
T13748	62765-62767	NN	denotes	ml
T13749	62767-62768	-RRB-	denotes	)
T13750	62769-62771	IN	denotes	at
T13751	62772-62774	CD	denotes	55
T13752	62775-62777	NN	denotes	°C
T13753	62778-62781	IN	denotes	for
T13754	62782-62784	CD	denotes	12
T13756	62784-62785	SYM	denotes	–
T13755	62785-62787	CD	denotes	14
T13757	62788-62789	NN	denotes	h
T13758	62789-62791	,	denotes	,
T13759	62791-62798	VBN	denotes	blocked
T13760	62799-62803	IN	denotes	with
T13761	62804-62806	CD	denotes	10
T13762	62806-62807	NN	denotes	%
T13763	62808-62811	NN	denotes	NGS
T13764	62811-62813	,	denotes	,
T13765	62813-62816	CC	denotes	and
T13766	62817-62824	VBN	denotes	treated
T13767	62825-62829	IN	denotes	with
T13768	62830-62838	JJ	denotes	anti-dig
T13770	62839-62842	NN	denotes	Fab
T13772	62842-62843	HYPH	denotes	-
T13771	62843-62845	NN	denotes	AP
T13769	62846-62854	NN	denotes	antibody
T13773	62855-62856	-LRB-	denotes	(
T13774	62856-62861	NNP	denotes	Roche
T13775	62862-62863	SYM	denotes	#
T13776	62863-62870	CD	denotes	1093274
T13777	62870-62871	-RRB-	denotes	)
T13778	62872-62874	IN	denotes	at
T13779	62875-62876	DT	denotes	a
T13781	62877-62878	CD	denotes	1
T13783	62878-62879	SYM	denotes	:
T13782	62879-62884	CD	denotes	2,500
T13780	62885-62893	NN	denotes	dilution
T13784	62894-62897	IN	denotes	for
T13785	62898-62899	CD	denotes	3
T13786	62900-62901	NN	denotes	h
T13787	62901-62902	.	denotes	.
T13788	62902-62984	sentence	denotes	The sections were incubated with NBT and BCIP until adequate signal was detected.
T13789	62903-62906	DT	denotes	The
T13790	62907-62915	NNS	denotes	sections
T13792	62916-62920	VBD	denotes	were
T13791	62921-62930	VBN	denotes	incubated
T13793	62931-62935	IN	denotes	with
T13794	62936-62939	NN	denotes	NBT
T13795	62940-62943	CC	denotes	and
T13796	62944-62948	NN	denotes	BCIP
T13797	62949-62954	IN	denotes	until
T13799	62955-62963	JJ	denotes	adequate
T13800	62964-62970	NN	denotes	signal
T13801	62971-62974	VBD	denotes	was
T13798	62975-62983	VBN	denotes	detected
T13802	62983-62984	.	denotes	.
T13828	62986-63004	NN	denotes	Immunofluorescence
T13829	63005-63008	CC	denotes	and
T13830	63009-63029	NN	denotes	immunohistochemistry
T13831	63029-63127	sentence	denotes	Tissue samples for immunofluorescence were frozen in OCT and sectioned 10 μm thick on a cryostat.
T13832	63030-63036	NN	denotes	Tissue
T13833	63037-63044	NNS	denotes	samples
T13835	63045-63048	IN	denotes	for
T13836	63049-63067	NN	denotes	immunofluorescence
T13837	63068-63072	VBD	denotes	were
T13834	63073-63079	VBN	denotes	frozen
T13838	63080-63082	IN	denotes	in
T13839	63083-63086	NN	denotes	OCT
T13840	63087-63090	CC	denotes	and
T13841	63091-63100	VBN	denotes	sectioned
T13842	63101-63103	CD	denotes	10
T13843	63104-63106	NN	denotes	μm
T13844	63107-63112	JJ	denotes	thick
T13845	63113-63115	IN	denotes	on
T13846	63116-63117	DT	denotes	a
T13847	63118-63126	NN	denotes	cryostat
T13848	63126-63127	.	denotes	.
T13849	63127-63240	sentence	denotes	Sections were fixed in 4% paraformaldehyde for 10 min at room temperature, blocked, and stained with antibodies.
T13850	63128-63136	NNS	denotes	Sections
T13852	63137-63141	VBD	denotes	were
T13851	63142-63147	VBN	denotes	fixed
T13853	63148-63150	IN	denotes	in
T13854	63151-63152	CD	denotes	4
T13855	63152-63153	NN	denotes	%
T13856	63154-63170	NN	denotes	paraformaldehyde
T13857	63171-63174	IN	denotes	for
T13858	63175-63177	CD	denotes	10
T13859	63178-63181	NN	denotes	min
T13860	63182-63184	IN	denotes	at
T13861	63185-63189	NN	denotes	room
T13862	63190-63201	NN	denotes	temperature
T13863	63201-63203	,	denotes	,
T13864	63203-63210	VBN	denotes	blocked
T13865	63210-63212	,	denotes	,
T13866	63212-63215	CC	denotes	and
T13867	63216-63223	VBN	denotes	stained
T13868	63224-63228	IN	denotes	with
T13869	63229-63239	NNS	denotes	antibodies
T13870	63239-63240	.	denotes	.
T13871	63240-63353	sentence	denotes	Tissue samples for immunohistochemistry were fixed in 4% paraformaldehyde, dehydrated, and embedded in paraffin.
T13872	63241-63247	NN	denotes	Tissue
T13873	63248-63255	NNS	denotes	samples
T13875	63256-63259	IN	denotes	for
T13876	63260-63280	NN	denotes	immunohistochemistry
T13877	63281-63285	VBD	denotes	were
T13874	63286-63291	VBN	denotes	fixed
T13878	63292-63294	IN	denotes	in
T13879	63295-63296	CD	denotes	4
T13880	63296-63297	NN	denotes	%
T13881	63298-63314	NN	denotes	paraformaldehyde
T13882	63314-63316	,	denotes	,
T13883	63316-63326	VBN	denotes	dehydrated
T13884	63326-63328	,	denotes	,
T13885	63328-63331	CC	denotes	and
T13886	63332-63340	VBN	denotes	embedded
T13887	63341-63343	IN	denotes	in
T13888	63344-63352	NN	denotes	paraffin
T13889	63352-63353	.	denotes	.
T13890	63353-63453	sentence	denotes	Samples were sectioned on a microtome (10 μm thick) and rehydrated prior to staining with antibody.
T13891	63354-63361	NNS	denotes	Samples
T13893	63362-63366	VBD	denotes	were
T13892	63367-63376	VBN	denotes	sectioned
T13894	63377-63379	IN	denotes	on
T13895	63380-63381	DT	denotes	a
T13896	63382-63391	NN	denotes	microtome
T13897	63392-63393	-LRB-	denotes	(
T13899	63393-63395	CD	denotes	10
T13900	63396-63398	NN	denotes	μm
T13898	63399-63404	JJ	denotes	thick
T13901	63404-63405	-RRB-	denotes	)
T13902	63406-63409	CC	denotes	and
T13903	63410-63420	VBD	denotes	rehydrated
T13904	63421-63426	IN	denotes	prior
T13905	63427-63429	IN	denotes	to
T13906	63430-63438	VBG	denotes	staining
T13907	63439-63443	IN	denotes	with
T13908	63444-63452	NN	denotes	antibody
T13909	63452-63453	.	denotes	.
T13910	63453-63639	sentence	denotes	Samples stained with Snail, pMAPK, pSmad2, and cyclin D were antigen unmasked with 10 mM sodium citrate (pH 6) in an Antigen Retriever 2100 (Pickcell Laboratories, Leiden, Netherlands).
T13911	63454-63461	NNS	denotes	Samples
T13913	63462-63469	VBN	denotes	stained
T13914	63470-63474	IN	denotes	with
T13915	63475-63480	NN	denotes	Snail
T13916	63480-63482	,	denotes	,
T13917	63482-63487	NN	denotes	pMAPK
T13918	63487-63489	,	denotes	,
T13919	63489-63495	NN	denotes	pSmad2
T13920	63495-63497	,	denotes	,
T13921	63497-63500	CC	denotes	and
T13922	63501-63507	NN	denotes	cyclin
T13923	63508-63509	NN	denotes	D
T13924	63510-63514	VBD	denotes	were
T13925	63515-63522	NN	denotes	antigen
T13912	63523-63531	VBN	denotes	unmasked
T13926	63532-63536	IN	denotes	with
T13927	63537-63539	CD	denotes	10
T13928	63540-63542	NN	denotes	mM
T13930	63543-63549	NN	denotes	sodium
T13929	63550-63557	NN	denotes	citrate
T13931	63558-63559	-LRB-	denotes	(
T13932	63559-63561	NN	denotes	pH
T13933	63562-63563	CD	denotes	6
T13934	63563-63564	-RRB-	denotes	)
T13935	63565-63567	IN	denotes	in
T13936	63568-63570	DT	denotes	an
T13938	63571-63578	NNP	denotes	Antigen
T13937	63579-63588	NNP	denotes	Retriever
T13939	63589-63593	CD	denotes	2100
T13940	63594-63595	-LRB-	denotes	(
T13942	63595-63603	NNP	denotes	Pickcell
T13941	63604-63616	NNP	denotes	Laboratories
T13943	63616-63618	,	denotes	,
T13944	63618-63624	NNP	denotes	Leiden
T13945	63624-63626	,	denotes	,
T13946	63626-63637	NNP	denotes	Netherlands
T13947	63637-63638	-RRB-	denotes	)
T13948	63638-63639	.	denotes	.
T13949	63639-63748	sentence	denotes	The DAB substrate kit (Vector Labs) was used according to manufacturer's instructions to develop the signal.
T13950	63640-63643	DT	denotes	The
T13952	63644-63647	NN	denotes	DAB
T13953	63648-63657	NN	denotes	substrate
T13951	63658-63661	NN	denotes	kit
T13955	63662-63663	-LRB-	denotes	(
T13957	63663-63669	NNP	denotes	Vector
T13956	63670-63674	NNPS	denotes	Labs
T13958	63674-63675	-RRB-	denotes	)
T13959	63676-63679	VBD	denotes	was
T13954	63680-63684	VBN	denotes	used
T13960	63685-63694	VBG	denotes	according
T13961	63695-63697	IN	denotes	to
T13962	63698-63710	NN	denotes	manufacturer
T13964	63710-63712	POS	denotes	's
T13963	63713-63725	NNS	denotes	instructions
T13965	63726-63728	TO	denotes	to
T13966	63729-63736	VB	denotes	develop
T13967	63737-63740	DT	denotes	the
T13968	63741-63747	NN	denotes	signal
T13969	63747-63748	.	denotes	.
T14008	63750-63752	NN	denotes	RT
T14010	63752-63753	HYPH	denotes	-
T14009	63753-63756	NN	denotes	PCR
T14011	63756-63875	sentence	denotes	RNA was extracted from keratinocytes or skin tissue with Trizol (Invitrogen) according to the manufacturer's protocol.
T14012	63757-63760	NN	denotes	RNA
T14014	63761-63764	VBD	denotes	was
T14013	63765-63774	VBN	denotes	extracted
T14015	63775-63779	IN	denotes	from
T14016	63780-63793	NNS	denotes	keratinocytes
T14017	63794-63796	CC	denotes	or
T14018	63797-63801	NN	denotes	skin
T14019	63802-63808	NN	denotes	tissue
T14020	63809-63813	IN	denotes	with
T14021	63814-63820	NN	denotes	Trizol
T14022	63821-63822	-LRB-	denotes	(
T14023	63822-63832	NNP	denotes	Invitrogen
T14024	63832-63833	-RRB-	denotes	)
T14025	63834-63843	VBG	denotes	according
T14026	63844-63846	IN	denotes	to
T14027	63847-63850	DT	denotes	the
T14028	63851-63863	NN	denotes	manufacturer
T14030	63863-63865	POS	denotes	's
T14029	63866-63874	NN	denotes	protocol
T14031	63874-63875	.	denotes	.
T14032	63875-63958	sentence	denotes	cDNA was generated using oligo-dT primers and the Superscript II kit (Invitrogen).
T14033	63876-63880	NN	denotes	cDNA
T14035	63881-63884	VBD	denotes	was
T14034	63885-63894	VBN	denotes	generated
T14036	63895-63900	VBG	denotes	using
T14037	63901-63906	NN	denotes	oligo
T14039	63906-63907	HYPH	denotes	-
T14038	63907-63909	NN	denotes	dT
T14040	63910-63917	NNS	denotes	primers
T14041	63918-63921	CC	denotes	and
T14042	63922-63925	DT	denotes	the
T14044	63926-63937	NNP	denotes	Superscript
T14045	63938-63940	CD	denotes	II
T14043	63941-63944	NN	denotes	kit
T14046	63945-63946	-LRB-	denotes	(
T14047	63946-63956	NNP	denotes	Invitrogen
T14048	63956-63957	-RRB-	denotes	)
T14049	63957-63958	.	denotes	.
T14050	63958-64171	sentence	denotes	The primers used for PCR were Snail forward: 5′- CAGCTGGCCAGGCTCTCGGT-3′; Snail reverse: 5′- GCGAGGGCCTCCGGAGCA-3′; GAPDH forward 5′- CGTAGACAAAATGGTGAAGGTCGG-3′; and GAPDH reverse: 5′- AAGCAGTTGGTGGTGCAGGATG-3′.
T14051	63959-63962	DT	denotes	The
T14052	63963-63970	NNS	denotes	primers
T14054	63971-63975	VBN	denotes	used
T14055	63976-63979	IN	denotes	for
T14056	63980-63983	NN	denotes	PCR
T14053	63984-63988	VBD	denotes	were
T14057	63989-63994	NN	denotes	Snail
T14058	63995-64002	NN	denotes	forward
T14059	64002-64004	:	denotes	:
T14060	64004-64005	CD	denotes	5
T14062	64005-64006	SYM	denotes	′
T14063	64006-64007	HYPH	denotes	-
T14061	64008-64028	NN	denotes	CAGCTGGCCAGGCTCTCGGT
T14064	64028-64029	HYPH	denotes	-
T14065	64029-64030	CD	denotes	3
T14066	64030-64031	SYM	denotes	′
T14067	64031-64032	:	denotes	;
T14068	64033-64038	NN	denotes	Snail
T14069	64039-64046	NN	denotes	reverse
T14070	64046-64048	:	denotes	:
T14071	64048-64049	CD	denotes	5
T14073	64049-64050	SYM	denotes	′
T14074	64050-64051	HYPH	denotes	-
T14072	64052-64070	NN	denotes	GCGAGGGCCTCCGGAGCA
T14075	64070-64071	HYPH	denotes	-
T14076	64071-64072	CD	denotes	3
T14077	64072-64073	SYM	denotes	′
T14078	64073-64074	:	denotes	;
T14079	64075-64080	NN	denotes	GAPDH
T14080	64081-64088	NN	denotes	forward
T14081	64089-64090	CD	denotes	5
T14083	64090-64091	SYM	denotes	′
T14084	64091-64092	HYPH	denotes	-
T14082	64093-64117	NN	denotes	CGTAGACAAAATGGTGAAGGTCGG
T14085	64117-64118	HYPH	denotes	-
T14086	64118-64119	CD	denotes	3
T14087	64119-64120	SYM	denotes	′
T14088	64120-64121	:	denotes	;
T14089	64122-64125	CC	denotes	and
T14090	64126-64131	NN	denotes	GAPDH
T14091	64132-64139	NN	denotes	reverse
T14092	64139-64141	:	denotes	:
T14093	64141-64142	CD	denotes	5
T14095	64142-64143	SYM	denotes	′
T14096	64143-64144	HYPH	denotes	-
T14094	64145-64167	NN	denotes	AAGCAGTTGGTGGTGCAGGATG
T14097	64167-64168	HYPH	denotes	-
T14098	64168-64169	CD	denotes	3
T14099	64169-64170	SYM	denotes	′
T14100	64170-64171	.	denotes	.
R10	T157	T158	compound	Hair,Follicle
R100	T254	T253	punct	-,activated
R1000	T1864	T1862	pobj	proximity,by
R1001	T1865	T1864	amod	close,proximity
R1002	T1757	T1756	pobj	proteins,of
R1003	T1866	T1864	prep	of,proximity
R1004	T1867	T1868	det	the,site
R1005	T1758	T1731	punct	),protein
R1006	T1759	T1731	punct	", ",protein
R1007	T1868	T1866	pobj	site,of
R1008	T1869	T1868	nmod	LEF,site
R1009	T1870	T1869	punct	-,LEF
R101	T255	T250	nmod	protein,kinase
R1010	T1760	T1761	dep	which,appears
R1011	T1871	T1869	nummod	1,LEF
R1012	T1872	T1869	punct	/,LEF
R1013	T1761	T1731	relcl	appears,protein
R1014	T1873	T1874	compound	β,catenin
R1015	T1762	T1763	aux	to,function
R1016	T1874	T1869	appos	catenin,LEF
R1017	T1875	T1874	punct	-,catenin
R1018	T1876	T1868	compound	binding,site
R1019	T1763	T1761	xcomp	function,appears
R102	T256	T250	punct	(,kinase
R1020	T1877	T1864	prep	to,proximity
R1021	T1878	T1879	det	a,site
R1022	T1879	T1877	pobj	site,to
R1023	T1764	T1763	advmod	dually,function
R1024	T1880	T1879	acl	known,site
R1025	T1881	T1882	aux	to,bind
R1026	T1765	T1763	prep	in,function
R1027	T1882	T1880	xcomp	bind,known
R1028	T1883	T1884	det	the,family
R1029	T1884	T1882	dobj	family,bind
R103	T257	T250	appos	MAPK,kinase
R1030	T1766	T1765	preconj	both,in
R1031	T1767	T1765	conj	adhesion,in
R1032	T1768	T1765	cc	and,in
R1033	T1769	T1765	conj	in,in
R1034	T1885	T1886	compound	Snail,Slug
R1035	T1886	T1884	compound	Slug,family
R1036	T1770	T1769	pobj	activation,in
R1037	T1887	T1886	punct	/,Slug
R1038	T1888	T1884	prep	of,family
R1039	T1889	T1890	nmod	zinc,finger
R104	T258	T248	punct	),pathway
R1040	T1771	T1770	prep	of,activation
R1041	T1890	T1891	nmod	finger,proteins
R1042	T1891	T1888	pobj	proteins,of
R1043	T1772	T1773	det	the,pathway
R1044	T1892	T1893	amod	transcriptional,repressor
R1045	T1893	T1891	compound	repressor,proteins
R1046	T1894	T1895	punct	[,15
R1047	T1773	T1771	pobj	pathway,of
R1048	T1895	T1846	parataxis	15,intrigued
R1049	T1896	T1895	nummod	12,15
R105	T259	T246	prep	in,activate
R1050	T1897	T1895	punct	",",15
R1051	T1898	T1895	nummod	13,15
R1052	T1899	T1895	punct	",",15
R1053	T1900	T1895	nummod	14,15
R1054	T1774	T1773	nmod	Ras,pathway
R1055	T1901	T1895	punct	",",15
R1056	T1902	T1895	punct	],15
R1057	T1903	T1846	punct	.,intrigued
R1058	T1775	T1774	punct	-,Ras
R1059	T1905	T1906	preconj	Both,activity
R106	T260	T261	det	the,bud
R1060	T1776	T1777	npadvmod	mitogen,activated
R1061	T1906	T1907	nsubj	activity,play
R1062	T1908	T1906	prep	of,activity
R1063	T1777	T1779	amod	activated,kinase
R1064	T1909	T1908	pobj	Snail,of
R1065	T1910	T1906	cc	and,activity
R1066	T1911	T1912	compound	down,regulation
R1067	T1778	T1777	punct	-,activated
R1068	T1912	T1906	conj	regulation,activity
R1069	T1913	T1912	punct	-,regulation
R107	T261	T259	pobj	bud,in
R1070	T1914	T1912	prep	of,regulation
R1071	T1779	T1774	appos	kinase,Ras
R1072	T1915	T1916	compound	E,cadherin
R1073	T1916	T1914	pobj	cadherin,of
R1074	T1991	T1988	acl	tune,ability
R1075	T1917	T1916	punct	-,cadherin
R1076	T1918	T1919	amod	pivotal,roles
R1077	T1919	T1907	dobj	roles,play
R1078	T1920	T1907	prep	in,play
R1079	T1992	T1991	advmod	fine,tune
R108	T262	T229	punct	.,found
R1080	T1921	T1922	amod	epithelial,transitions
R1081	T1922	T1920	pobj	transitions,in
R1082	T1923	T1921	prep	to,epithelial
R1083	T1993	T1991	punct	-,tune
R1084	T1924	T1923	amod	mesenchymal,to
R1085	T1925	T1922	punct	(,transitions
R1086	T1994	T1995	compound	cadherin,expression
R1087	T1926	T1922	appos	EMTs,transitions
R1088	T1927	T1922	punct	),transitions
R1089	T1928	T1922	punct	", ",transitions
R109	T264	T265	prep	In,leads
R1090	T1995	T1991	dobj	expression,tune
R1091	T1929	T1922	acl	typified,transitions
R1092	T1930	T1929	agent	by,typified
R1093	T1931	T1932	det	the,transformation
R1094	T1996	T1991	prep	at,tune
R1095	T1932	T1930	pobj	transformation,by
R1096	T1933	T1932	prep	of,transformation
R1097	T1934	T1935	amod	polarized,cells
R1098	T1997	T1998	det	the,level
R1099	T1935	T1933	pobj	cells,of
R11	T158	T159	compound	Follicle,Morphogenesis
R110	T266	T267	det	the,epidermis
R1100	T1936	T1935	punct	", ",cells
R1101	T1937	T1935	amod	adhering,cells
R1102	T1938	T1935	amod	epithelial,cells
R1103	T1939	T1932	prep	into,transformation
R1104	T1998	T1996	pobj	level,at
R1105	T1940	T1941	amod	motile,cells
R1106	T1941	T1939	pobj	cells,into
R1107	T1999	T1998	amod	transcriptional,level
R1108	T1942	T1941	amod	mesenchymal,cells
R1109	T1943	T1944	punct	[,17
R111	T267	T264	pobj	epidermis,In
R1110	T1944	T1907	parataxis	17,play
R1111	T2000	T1985	punct	", ",seems
R1112	T1945	T1944	nummod	16,17
R1113	T1946	T1944	punct	",",17
R1114	T1947	T1944	punct	],17
R1115	T2001	T1985	nsubj	Snail,seems
R1116	T1948	T1907	punct	.,play
R1117	T1950	T1951	compound	Bud,formation
R1118	T2002	T2003	aux	to,have
R1119	T1951	T1952	nsubj	formation,differs
R112	T268	T265	punct	", ",leads
R1120	T2003	T1985	xcomp	have,seems
R1121	T1953	T1952	prep	from,differs
R1122	T1954	T1955	det	an,EMT
R1123	T2004	T2005	det	an,effect
R1124	T1955	T1953	pobj	EMT,from
R1125	T1956	T1957	mark	in,needs
R1126	T2005	T2003	dobj	effect,have
R1127	T2006	T2005	amod	additive,effect
R1128	T1957	T1952	advcl	needs,differs
R1129	T1958	T1957	mark	that,needs
R113	T269	T270	compound	Snail,misexpression
R1130	T1959	T1960	compound	E,cadherin
R1131	T2007	T2003	prep	with,have
R1132	T1960	T1962	compound	cadherin,activity
R1133	T1961	T1960	punct	-,cadherin
R1134	T1962	T1957	nsubj	activity,needs
R1135	T2008	T2007	pobj	LEF,with
R1136	T1963	T1964	aux	to,regulated
R1137	T1964	T1957	xcomp	regulated,needs
R1138	T1965	T1964	aux	be,regulated
R1139	T2009	T2008	punct	-,LEF
R114	T270	T265	nsubj	misexpression,leads
R1140	T1966	T1964	advmod	down,regulated
R1141	T1967	T1964	punct	-,regulated
R1142	T2010	T2008	nummod	1,LEF
R1143	T1968	T1964	cc	but,regulated
R1144	T1969	T1968	neg	not,but
R1145	T2011	T2008	punct	/,LEF
R1146	T1970	T1964	conj	prevented,regulated
R1147	T1971	T1964	punct	", ",regulated
R1148	T1972	T1973	mark	so,remodeled
R1149	T2012	T2013	compound	β,catenin
R115	T271	T265	prep	to,leads
R1150	T1973	T1964	advcl	remodeled,regulated
R1151	T1974	T1973	mark	that,remodeled
R1152	T2013	T2008	appos	catenin,LEF
R1153	T1975	T1976	amod	adhesive,junctions
R1154	T1976	T1973	nsubjpass	junctions,remodeled
R1155	T1977	T1973	auxpass	are,remodeled
R1156	T1978	T1979	advmod	rather,than
R1157	T1979	T1973	cc	than,remodeled
R1158	T1980	T1981	advmod	quantitatively,impaired
R1159	T1981	T1973	conj	impaired,remodeled
R116	T272	T271	pobj	hyperproliferation,to
R1160	T2014	T2013	punct	-,catenin
R1161	T1982	T1952	punct	.,differs
R1162	T2015	T2003	prep	in,have
R1163	T1984	T1985	advcl	Supportive,seems
R1164	T1986	T1984	prep	of,Supportive
R1165	T2016	T2017	advmod	negatively,modulating
R1166	T1987	T1988	det	an,ability
R1167	T1988	T1986	pobj	ability,of
R1168	T1989	T1988	amod	underlying,ability
R1169	T2017	T2015	pcomp	modulating,in
R117	T273	T272	cc	and,hyperproliferation
R1170	T1990	T1991	aux	to,tune
R1171	T2018	T2019	compound	E,cadherin
R1172	T2019	T2021	compound	cadherin,promoter
R1173	T2020	T2019	punct	-,cadherin
R1174	T2021	T2022	compound	promoter,activity
R1175	T2022	T2017	dobj	activity,modulating
R1176	T2023	T2024	punct	[,4
R1177	T2097	T2096	acl	associated,features
R1178	T2098	T2097	prep	with,associated
R1179	T2024	T1985	parataxis	4,seems
R118	T274	T275	det	a,reduction
R1180	T2099	T2100	compound	hair,bud
R1181	T2100	T2101	compound	bud,formation
R1182	T2025	T2024	punct	],4
R1183	T2101	T2098	pobj	formation,with
R1184	T2102	T2096	punct	", ",features
R1185	T2103	T2096	prep	including,features
R1186	T2026	T1985	punct	.,seems
R1187	T2104	T2105	compound	down,regulation
R1188	T2105	T2103	pobj	regulation,including
R1189	T2028	T2029	prep	In,discovered
R119	T275	T272	conj	reduction,hyperproliferation
R1190	T2106	T2105	punct	-,regulation
R1191	T2107	T2105	prep	of,regulation
R1192	T2108	T2109	compound	E,cadherin
R1193	T2109	T2107	pobj	cadherin,of
R1194	T2030	T2031	det	the,study
R1195	T2110	T2109	punct	-,cadherin
R1196	T2111	T2105	punct	", ",regulation
R1197	T2112	T2113	amod	increased,proliferation
R1198	T2113	T2105	conj	proliferation,regulation
R1199	T2031	T2028	pobj	study,In
R12	T159	T156	pobj	Morphogenesis,in
R120	T276	T275	prep	in,reduction
R1200	T2114	T2113	punct	", ",proliferation
R1201	T2115	T2113	cc	and,proliferation
R1202	T2032	T2031	amod	present,study
R1203	T2116	T2117	amod	repressed,differentiation
R1204	T2117	T2113	conj	differentiation,proliferation
R1205	T2118	T2117	amod	terminal,differentiation
R1206	T2119	T2084	punct	.,appears
R1207	T2033	T2029	punct	", ",discovered
R1208	T2121	T2122	mark	Although,reflected
R1209	T2034	T2029	nsubj	we,discovered
R121	T277	T278	amod	intercellular,adhesion
R1210	T2122	T2133	advcl	reflected,appear
R1211	T2123	T2124	det	the,spike
R1212	T2035	T2036	mark	that,expressed
R1213	T2124	T2122	nsubjpass	spike,reflected
R1214	T2125	T2124	amod	temporal,spike
R1215	T2036	T2029	ccomp	expressed,discovered
R1216	T2126	T2124	prep	of,spike
R1217	T2127	T2126	pobj	Snail,of
R1218	T2037	T2036	nsubjpass	Snail,expressed
R1219	T2128	T2124	prep	in,spike
R122	T278	T276	pobj	adhesion,in
R1220	T2129	T2130	det	the,bud
R1221	T2130	T2128	pobj	bud,in
R1222	T2038	T2036	auxpass	is,expressed
R1223	T2131	T2130	compound	hair,bud
R1224	T2132	T2122	auxpass	is,reflected
R1225	T2039	T2036	advmod	briefly,expressed
R1226	T2134	T2122	prep	at,reflected
R1227	T2135	T2136	det	the,level
R1228	T2136	T2134	pobj	level,at
R1229	T2040	T2036	prep	at,expressed
R123	T279	T265	punct	.,leads
R1230	T2137	T2136	compound	mRNA,level
R1231	T2138	T2122	cc	and,reflected
R1232	T2139	T2122	conj	seems,reflected
R1233	T2041	T2042	det	an,stage
R1234	T2140	T2141	aux	to,follow
R1235	T2141	T2139	xcomp	follow,seems
R1236	T2142	T2143	compound	Wnt,signaling
R1237	T2042	T2040	pobj	stage,at
R1238	T2143	T2141	dobj	signaling,follow
R1239	T2144	T2143	cc	and,signaling
R124	T281	T282	advmod	When,regulated
R1240	T2043	T2042	amod	early,stage
R1241	T2145	T2146	compound	BMP,inhibition
R1242	T2146	T2143	conj	inhibition,signaling
R1243	T2147	T2133	punct	", ",appear
R1244	T2044	T2042	prep	of,stage
R1245	T2148	T2149	nmod	LEF,activation
R1246	T2149	T2133	nsubj	activation,appear
R1247	T2045	T2046	compound	hair,bud
R1248	T2150	T2148	punct	-,LEF
R1249	T2151	T2148	nummod	1,LEF
R125	T282	T290	advcl	regulated,released
R1250	T2152	T2148	punct	/,LEF
R1251	T2046	T2047	compound	bud,formation
R1252	T2153	T2154	compound	β,catenin
R1253	T2154	T2148	appos	catenin,LEF
R1254	T2155	T2154	punct	-,catenin
R1255	T2047	T2044	pobj	formation,of
R1256	T2156	T2133	aux	does,appear
R1257	T2157	T2133	neg	not,appear
R1258	T2158	T2159	aux	to,induce
R1259	T2159	T2133	xcomp	induce,appear
R126	T283	T284	compound	E,cadherin
R1260	T2160	T2161	compound	Snail,expression
R1261	T2161	T2159	dobj	expression,induce
R1262	T2162	T2161	compound	gene,expression
R1263	T2048	T2042	punct	", ",stage
R1264	T2163	T2159	prep	in,induce
R1265	T2164	T2165	amod	embryonic,keratinocytes
R1266	T2165	T2163	pobj	keratinocytes,in
R1267	T2166	T2165	compound	skin,keratinocytes
R1268	T2049	T2050	advmod	when,take
R1269	T2167	T2133	punct	.,appear
R127	T284	T282	nsubjpass	cadherin,regulated
R1270	T2050	T2042	relcl	take,stage
R1271	T2169	T2170	prep	In,provide
R1272	T2171	T2169	pobj	contrast,In
R1273	T2051	T2052	compound	E,cadherin
R1274	T2172	T2170	punct	", ",provide
R1275	T2173	T2170	nsubj	we,provide
R1276	T2174	T2175	advmod	in,vitro
R1277	T2052	T2050	nsubj	cadherin,take
R1278	T2175	T2176	amod	vitro,evidence
R1279	T2176	T2170	dobj	evidence,provide
R128	T285	T284	punct	-,cadherin
R1280	T2053	T2052	punct	-,cadherin
R1281	T2177	T2175	punct	", ",vitro
R1282	T2178	T2175	conj	transgenic,vitro
R1283	T2179	T2180	punct	(,Tg
R1284	T2054	T2055	amod	down,regulation
R1285	T2180	T2178	parataxis	Tg,transgenic
R1286	T2181	T2180	punct	),Tg
R1287	T2182	T2178	punct	", ",transgenic
R1288	T2055	T2052	appos	regulation,cadherin
R1289	T2183	T2178	cc	and,transgenic
R129	T286	T282	auxpass	is,regulated
R1290	T2184	T2185	npadvmod	gene,targeting
R1291	T2056	T2055	punct	-,regulation
R1292	T2185	T2178	conj	targeting,transgenic
R1293	T2186	T2187	aux	to,show
R1294	T2057	T2055	cc	and,regulation
R1295	T2187	T2170	advcl	show,provide
R1296	T2188	T2189	mark	that,are
R1297	T2058	T2055	conj	activation,regulation
R1298	T2059	T2058	prep	of,activation
R1299	T2189	T2187	ccomp	are,show
R13	T162	T163	prep	In,exchange
R130	T287	T282	advmod	transcriptionally,regulated
R1300	T2190	T2191	nmod	TGF,β2
R1301	T2060	T2059	pobj	proliferation,of
R1302	T2191	T2193	nmod	β2,signaling
R1303	T2192	T2191	punct	-,β2
R1304	T2193	T2189	nsubj	signaling,are
R1305	T2061	T2050	dobj	place,take
R1306	T2194	T2191	cc	and,β2
R1307	T2195	T2196	amod	small,phenotype
R1308	T2196	T2197	npadvmod	phenotype,related
R1309	T2197	T2204	amod	related,protein
R131	T288	T282	advmod	down,regulated
R1310	T2198	T2196	punct	–,phenotype
R1311	T2199	T2196	cc	and,phenotype
R1312	T2062	T2029	punct	.,discovered
R1313	T2200	T2196	conj	mothers,phenotype
R1314	T2201	T2200	prep	against,mothers
R1315	T2202	T2201	pobj	decapentaplegic,against
R1316	T2064	T2065	advmod	Thereafter,disappears
R1317	T2066	T2065	punct	", ",disappears
R1318	T2203	T2197	punct	–,related
R1319	T2204	T2191	conj	protein,β2
R132	T289	T282	punct	-,regulated
R1320	T2067	T2065	nsubj	Snail,disappears
R1321	T2205	T2204	nummod	2,protein
R1322	T2206	T2204	punct	(,protein
R1323	T2207	T2204	appos	SMAD2,protein
R1324	T2068	T2065	cc	and,disappears
R1325	T2208	T2193	punct	),signaling
R1326	T2209	T2210	amod	upstream,inducers
R1327	T2069	T2065	conj	remains,disappears
R1328	T2210	T2189	attr	inducers,are
R1329	T2211	T2210	prep	of,inducers
R133	T291	T290	punct	", ",released
R1330	T2212	T2213	compound	Snail,expression
R1331	T2070	T2069	acomp	absent,remains
R1332	T2213	T2211	pobj	expression,of
R1333	T2214	T2213	compound	gene,expression
R1334	T2071	T2069	prep	during,remains
R1335	T2215	T2189	prep	in,are
R1336	T2216	T2217	compound	skin,epithelium
R1337	T2072	T2073	amod	subsequent,growth
R1338	T2217	T2215	pobj	epithelium,in
R1339	T2218	T2170	punct	.,provide
R134	T292	T293	amod	associated,proteins
R1340	T2073	T2071	pobj	growth,during
R1341	T2220	T2221	prep	In,form
R1342	T2074	T2073	nmod	follicle,growth
R1343	T2222	T2223	det	the,absence
R1344	T2223	T2220	pobj	absence,In
R1345	T2075	T2073	amod	down,growth
R1346	T2224	T2223	prep	of,absence
R1347	T2225	T2226	compound	TGF,β2
R1348	T2226	T2228	compound	β2,signaling
R1349	T2076	T2073	punct	-,growth
R135	T293	T290	nsubjpass	proteins,released
R1350	T2227	T2226	punct	-,β2
R1351	T2077	T2073	cc	and,growth
R1352	T2228	T2224	pobj	signaling,of
R1353	T2229	T2228	cc	and,signaling
R1354	T2230	T2231	compound	Snail,expression
R1355	T2078	T2073	conj	maturation,growth
R1356	T2231	T2228	conj	expression,signaling
R1357	T2232	T2231	compound	gene,expression
R1358	T2079	T2065	punct	.,disappears
R1359	T2233	T2221	punct	", ",form
R136	T294	T293	compound	adhesion,proteins
R1360	T2234	T2235	compound	hair,placodes
R1361	T2235	T2221	nsubj	placodes,form
R1362	T2236	T2221	aux	can,form
R1363	T2237	T2221	punct	", ",form
R1364	T2238	T2221	cc	but,form
R1365	T2081	T2082	det	This,pattern
R1366	T2239	T2240	amod	further,growth
R1367	T2240	T2244	nsubjpass	growth,blocked
R1368	T2241	T2240	nmod	follicle,growth
R1369	T2242	T2240	amod	down,growth
R137	T295	T293	prep	with,proteins
R1370	T2082	T2084	nsubj	pattern,appears
R1371	T2243	T2240	punct	-,growth
R1372	T2244	T2221	conj	blocked,form
R1373	T2245	T2244	auxpass	is,blocked
R1374	T2083	T2082	amod	exquisite,pattern
R1375	T2246	T2221	punct	.,form
R1376	T2085	T2086	aux	to,be
R1377	T2248	T2249	poss	Our,studies
R1378	T2249	T2250	nsubj	studies,point
R1379	T2251	T2250	prep	to,point
R138	T296	T297	amod	dual,functions
R1380	T2086	T2084	xcomp	be,appears
R1381	T2252	T2253	det	the,view
R1382	T2253	T2251	pobj	view,to
R1383	T2087	T2088	advmod	functionally,relevant
R1384	T2254	T2255	mark	that,functions
R1385	T2255	T2253	acl	functions,view
R1386	T2256	T2255	nsubj	Snail,functions
R1387	T2088	T2086	acomp	relevant,be
R1388	T2257	T2255	advmod	likely,functions
R1389	T2258	T2255	advmod	downstream,functions
R139	T297	T295	pobj	functions,with
R1390	T2259	T2258	prep	of,downstream
R1391	T2089	T2090	mark	since,affects
R1392	T2260	T2261	compound	cell,fate
R1393	T2261	T2262	compound	fate,specification
R1394	T2090	T2086	advcl	affects,be
R1395	T2262	T2259	pobj	specification,of
R1396	T2263	T2250	punct	", ",point
R1397	T2264	T2250	prep	at,point
R1398	T2091	T2090	csubj	altering,affects
R1399	T2265	T2266	det	a,stage
R14	T164	T165	det	a,theme
R140	T298	T297	prep	in,functions
R1400	T2266	T2264	pobj	stage,at
R1401	T2092	T2091	dobj	it,altering
R1402	T2267	T2268	advmod	where,begins
R1403	T2268	T2266	relcl	begins,stage
R1404	T2093	T2094	advmod	in,vivo
R1405	T2269	T2270	det	the,bud
R1406	T2270	T2268	nsubj	bud,begins
R1407	T2094	T2091	advmod	vivo,altering
R1408	T2271	T2272	aux	to,exhibit
R1409	T2272	T2268	xcomp	exhibit,begins
R141	T299	T298	pobj	signaling,in
R1410	T2273	T2274	amod	enhanced,proliferation
R1411	T2095	T2090	advmod	correspondingly,affects
R1412	T2274	T2272	dobj	proliferation,exhibit
R1413	T2275	T2274	cc	and,proliferation
R1414	T2276	T2274	conj	migration,proliferation
R1415	T2277	T2250	punct	.,point
R1416	T2096	T2090	dobj	features,affects
R142	T300	T290	auxpass	are,released
R143	T301	T290	prep	from,released
R1433	T2702	T2703	compound	Snail,mRNA
R1434	T2703	T2704	nsubjpass	mRNA,Expressed
R1435	T2705	T2703	cc	and,mRNA
R1436	T2706	T2703	conj	Protein,mRNA
R1437	T2707	T2704	auxpass	Are,Expressed
R1438	T2708	T2704	advmod	Transiently,Expressed
R1439	T2709	T2704	prep	at,Expressed
R144	T302	T303	compound	cell,cell
R1440	T2710	T2711	det	the,Stage
R1441	T2711	T2709	pobj	Stage,at
R1442	T2712	T2713	compound	Hair,Bud
R1443	T2713	T2711	compound	Bud,Stage
R1444	T2714	T2711	prep	of,Stage
R1445	T2715	T2716	compound	Follicle,Morphogenesis
R1446	T2716	T2714	pobj	Morphogenesis,of
R1447	T2718	T2719	mark	Although,associated
R1448	T2719	T2726	advcl	associated,participate
R1449	T2720	T2721	compound	Snail,members
R145	T303	T305	compound	cell,contacts
R1450	T2721	T2719	nsubjpass	members,associated
R1451	T2722	T2721	compound	family,members
R1452	T2723	T2719	auxpass	are,associated
R1453	T2724	T2725	advmod	most,frequently
R1454	T2725	T2719	advmod	frequently,associated
R1455	T2727	T2719	prep	with,associated
R1456	T2728	T2727	pobj	EMTs,with
R1457	T2729	T2726	punct	", ",participate
R1458	T2730	T2726	nsubj	they,participate
R1459	T2731	T2726	advmod	also,participate
R146	T304	T303	punct	-,cell
R1460	T2732	T2726	prep	in,participate
R1461	T2733	T2734	amod	many,processes
R1462	T2734	T2732	pobj	processes,in
R1463	T2735	T2734	amod	malignant,processes
R1464	T2736	T2734	acl	involving,processes
R1465	T2737	T2738	det	a,regulation
R1466	T2738	T2736	dobj	regulation,involving
R1467	T2739	T2738	amod	down,regulation
R1468	T2740	T2738	punct	-,regulation
R1469	T2741	T2738	cc	but,regulation
R147	T305	T301	pobj	contacts,from
R1470	T2742	T2741	neg	not,but
R1471	T2743	T2744	det	a,abrogation
R1472	T2744	T2738	conj	abrogation,regulation
R1473	T2745	T2744	amod	quantitative,abrogation
R1474	T2746	T2738	prep	of,regulation
R1475	T2747	T2748	amod	intercellular,junctions
R1476	T2748	T2746	pobj	junctions,of
R1477	T2749	T2750	punct	[,18
R1478	T2750	T2726	parataxis	18,participate
R1479	T2751	T2750	punct	],18
R148	T306	T290	punct	", ",released
R1480	T2752	T2726	punct	.,participate
R1481	T2754	T2755	det	The,range
R1482	T2755	T2756	nsubj	range,includes
R1483	T2757	T2755	prep	of,range
R1484	T2758	T2759	amod	developmental,processes
R1485	T2759	T2757	pobj	processes,of
R1486	T2760	T2761	prep	in,implicated
R1487	T2761	T2759	relcl	implicated,processes
R1488	T2762	T2760	pobj	which,in
R1489	T2763	T2764	compound	Snail,members
R149	T307	T308	det	a,process
R1490	T2764	T2761	nsubjpass	members,implicated
R1491	T2765	T2764	compound	family,members
R1492	T2766	T2761	aux	have,implicated
R1493	T2767	T2761	auxpass	been,implicated
R1494	T2768	T2756	advmod	thus,includes
R1495	T2769	T2770	det	the,type
R1496	T2770	T2756	dobj	type,includes
R1497	T2771	T2770	prep	of,type
R1498	T2772	T2773	amod	epithelial,remodeling
R1499	T2773	T2771	pobj	remodeling,of
R15	T165	T162	pobj	theme,In
R150	T308	T290	npadvmod	process,released
R1500	T2774	T2775	dep	that,observed
R1501	T2775	T2770	relcl	observed,type
R1502	T2776	T2775	auxpass	is,observed
R1503	T2777	T2775	prep	in,observed
R1504	T2778	T2779	compound	hair,follicle
R1505	T2779	T2780	compound	follicle,bud
R1506	T2780	T2781	compound	bud,formation
R1507	T2781	T2777	pobj	formation,in
R1508	T2782	T2756	punct	.,includes
R1509	T2784	T2785	prep	Given,turned
R151	T309	T310	dep	which,demonstrate
R1510	T2786	T2787	poss	our,observation
R1511	T2787	T2784	pobj	observation,Given
R1512	T2788	T2787	amod	prior,observation
R1513	T2789	T2790	mark	that,participate
R1514	T2790	T2787	acl	participate,observation
R1515	T2791	T2792	advmod	exogenously,added
R1516	T2792	T2793	amod	added,Snail
R1517	T2793	T2790	nsubj	Snail,participate
R1518	T2794	T2790	aux	can,participate
R1519	T2795	T2790	prep	with,participate
R152	T310	T308	relcl	demonstrate,process
R1520	T2796	T2795	pobj	LEF,with
R1521	T2797	T2796	punct	-,LEF
R1522	T2798	T2796	nummod	1,LEF
R1523	T2799	T2796	punct	/,LEF
R1524	T2800	T2801	compound	β,catenin
R1525	T2801	T2796	appos	catenin,LEF
R1526	T2802	T2801	punct	-,catenin
R1527	T2803	T2790	prep	in,participate
R1528	T2804	T2805	advmod	down,regulating
R1529	T2805	T2803	pcomp	regulating,in
R153	T311	T310	nsubj	we,demonstrate
R1530	T2806	T2805	punct	-,regulating
R1531	T2807	T2808	compound	E,cadherin
R1532	T2808	T2810	compound	cadherin,expression
R1533	T2809	T2808	punct	-,cadherin
R1534	T2810	T2805	dobj	expression,regulating
R1535	T2811	T2790	prep	in,participate
R1536	T2812	T2811	pobj	keratinocytes,in
R1537	T2813	T2814	punct	[,4
R1538	T2814	T2790	parataxis	4,participate
R1539	T2815	T2814	punct	],4
R154	T312	T310	advcl	leads,demonstrate
R1540	T2816	T2787	punct	", ",observation
R1541	T2817	T2787	acl	coupled,observation
R1542	T2818	T2817	prep	with,coupled
R1543	T2819	T2820	det	the,requirement
R1544	T2820	T2818	pobj	requirement,with
R1545	T2821	T2820	amod	established,requirement
R1546	T2822	T2820	prep	for,requirement
R1547	T2823	T2822	pobj	LEF,for
R1548	T2824	T2823	punct	-,LEF
R1549	T2825	T2823	nummod	1,LEF
R155	T313	T312	prep	to,leads
R1550	T2826	T2823	punct	/,LEF
R1551	T2827	T2828	compound	β,catenin
R1552	T2828	T2823	appos	catenin,LEF
R1553	T2829	T2828	punct	-,catenin
R1554	T2830	T2820	prep	in,requirement
R1555	T2831	T2832	compound	hair,follicle
R1556	T2832	T2833	compound	follicle,morphogenesis
R1557	T2833	T2830	pobj	morphogenesis,in
R1558	T2834	T2835	punct	[,19
R1559	T2835	T2817	parataxis	19,coupled
R156	T314	T315	compound	Ras,MAPK
R1560	T2836	T2835	nummod	4,19
R1561	T2837	T2835	punct	",",19
R1562	T2838	T2835	punct	],19
R1563	T2839	T2785	punct	", ",turned
R1564	T2840	T2785	nsubj	we,turned
R1565	T2841	T2785	prep	to,turned
R1566	T2842	T2841	pcomp	addressing,to
R1567	T2843	T2844	mark	whether,participate
R1568	T2844	T2842	ccomp	participate,addressing
R1569	T2845	T2846	compound	Snail,Slug
R157	T315	T317	compound	MAPK,activation
R1570	T2846	T2848	compound	Slug,members
R1571	T2847	T2846	punct	/,Slug
R1572	T2848	T2844	nsubj	members,participate
R1573	T2849	T2848	compound	family,members
R1574	T2850	T2844	aux	might,participate
R1575	T2851	T2844	advmod	also,participate
R1576	T2852	T2844	prep	in,participate
R1577	T2853	T2854	det	the,process
R1578	T2854	T2852	pobj	process,in
R1579	T2855	T2785	punct	.,turned
R158	T316	T315	punct	-,MAPK
R1580	T2857	T2858	compound	PCR,analyses
R1581	T2858	T2859	nsubj	analyses,identified
R1582	T2860	T2861	amod	transient,expression
R1583	T2861	T2859	dobj	expression,identified
R1584	T2862	T2861	compound	Snail,expression
R1585	T2863	T2861	compound	mRNA,expression
R1586	T2864	T2859	prep	during,identified
R1587	T2865	T2866	det	a,period
R1588	T2866	T2864	pobj	period,during
R1589	T2867	T2866	prep	of,period
R159	T317	T313	pobj	activation,to
R1590	T2868	T2869	compound	skin,embryogenesis
R1591	T2869	T2867	pobj	embryogenesis,of
R1592	T2870	T2871	advmod	when,forming
R1593	T2871	T2866	advcl	forming,period
R1594	T2872	T2871	nsubj	waves,forming
R1595	T2873	T2872	prep	of,waves
R1596	T2874	T2875	compound	hair,follicles
R1597	T2875	T2873	pobj	follicles,of
R1598	T2876	T2871	aux	are,forming
R1599	T2877	T2878	punct	(,data
R16	T166	T165	amod	common,theme
R160	T318	T290	punct	.,released
R1600	T2878	T2859	meta	data,identified
R1601	T2879	T2878	amod	unpublished,data
R1602	T2880	T2878	punct	),data
R1603	T2881	T2859	punct	.,identified
R1604	T2882	T2883	aux	To,pinpoint
R1605	T2883	T2885	advcl	pinpoint,conducted
R1606	T2886	T2883	advmod	specifically,pinpoint
R1607	T2887	T2888	advmod	where,expressed
R1608	T2888	T2883	advcl	expressed,pinpoint
R1609	T2889	T2890	compound	Snail,mRNA
R161	T320	T321	det	These,studies
R1610	T2890	T2888	nsubjpass	mRNA,expressed
R1611	T2891	T2888	auxpass	is,expressed
R1612	T2892	T2888	prep	in,expressed
R1613	T2893	T2894	det	the,skin
R1614	T2894	T2892	pobj	skin,in
R1615	T2895	T2894	amod	developing,skin
R1616	T2896	T2885	punct	", ",conducted
R1617	T2897	T2885	nsubj	we,conducted
R1618	T2898	T2899	advmod	in,situ
R1619	T2899	T2900	amod	situ,hybridization
R162	T321	T322	nsubj	studies,provide
R1620	T2900	T2885	dobj	hybridization,conducted
R1621	T2901	T2885	advcl	using,conducted
R1622	T2902	T2903	det	a,probe
R1623	T2903	T2901	dobj	probe,using
R1624	T2904	T2903	compound	cRNA,probe
R1625	T2905	T2903	amod	unique,probe
R1626	T2906	T2905	prep	to,unique
R1627	T2907	T2908	det	the,region
R1628	T2908	T2906	pobj	region,to
R1629	T2909	T2908	nmod	Snail,region
R163	T323	T322	dobj	insights,provide
R1630	T2910	T2908	nummod	3,region
R1631	T2911	T2910	punct	′,3
R1632	T2912	T2908	amod	untranslated,region
R1633	T2913	T2908	punct	(,region
R1634	T2914	T2908	appos	UTR,region
R1635	T2915	T2908	punct	),region
R1636	T2916	T2885	punct	.,conducted
R1637	T2918	T2919	amod	Embryonic,day
R1638	T2919	T2920	nsubjpass	day,chosen
R1639	T2921	T2919	nummod	17.5,day
R164	T324	T323	prep	into,insights
R1640	T2922	T2919	punct	(,day
R1641	T2923	T2919	appos	E17.5,day
R1642	T2924	T2920	punct	),chosen
R1643	T2925	T2920	auxpass	was,chosen
R1644	T2926	T2920	punct	", ",chosen
R1645	T2927	T2928	mark	since,enabled
R1646	T2928	T2920	advcl	enabled,chosen
R1647	T2929	T2930	det	the,waves
R1648	T2930	T2928	nsubj	waves,enabled
R1649	T2931	T2930	amod	multiple,waves
R165	T325	T326	advmod	how,stimulated
R1650	T2932	T2930	prep	of,waves
R1651	T2933	T2934	compound	follicle,morphogenesis
R1652	T2934	T2932	pobj	morphogenesis,of
R1653	T2935	T2930	acl	occurring,waves
R1654	T2936	T2935	prep	at,occurring
R1655	T2937	T2938	det	this,time
R1656	T2938	T2936	pobj	time,at
R1657	T2939	T2928	dobj	us,enabled
R1658	T2940	T2941	aux	to,evaluate
R1659	T2941	T2928	xcomp	evaluate,enabled
R166	T326	T324	pcomp	stimulated,into
R1660	T2942	T2943	compound	Snail,expression
R1661	T2943	T2941	dobj	expression,evaluate
R1662	T2944	T2941	prep	at,evaluate
R1663	T2945	T2946	amod	different,stages
R1664	T2946	T2944	pobj	stages,at
R1665	T2947	T2946	prep	of,stages
R1666	T2948	T2949	det	the,process
R1667	T2949	T2947	pobj	process,of
R1668	T2950	T2920	punct	.,chosen
R1669	T2952	T2953	mark	As,shown
R167	T327	T328	amod	multipotent,cells
R1670	T2953	T2954	advcl	shown,detected
R1671	T2955	T2953	prep	in,shown
R1672	T2956	T2957	compound	Figure,1A
R1673	T2957	T2955	pobj	1A,in
R1674	T2958	T2954	punct	", ",detected
R1675	T2959	T2960	amod	specific,hybridization
R1676	T2960	T2954	nsubjpass	hybridization,detected
R1677	T2961	T2954	auxpass	was,detected
R1678	T2962	T2954	prep	within,detected
R1679	T2963	T2964	det	the,epithelium
R168	T328	T326	nsubjpass	cells,stimulated
R1680	T2964	T2962	pobj	epithelium,within
R1681	T2965	T2964	prep	of,epithelium
R1682	T2966	T2967	amod	nascent,buds
R1683	T2967	T2965	pobj	buds,of
R1684	T2968	T2967	compound	hair,buds
R1685	T2969	T2954	punct	.,detected
R1686	T2971	T2972	prep	By,exhibited
R1687	T2973	T2971	pobj	contrast,By
R1688	T2974	T2972	punct	", ",exhibited
R1689	T2975	T2976	mark	as,progressed
R169	T329	T328	prep	within,cells
R1690	T2976	T2972	advcl	progressed,exhibited
R1691	T2977	T2976	nsubj	follicles,progressed
R1692	T2978	T2976	advmod	further,progressed
R1693	T2979	T2976	prep	through,progressed
R1694	T2980	T2981	poss	their,development
R1695	T2981	T2979	pobj	development,through
R1696	T2982	T2983	punct	(,stages
R1697	T2983	T2976	parataxis	stages,progressed
R1698	T2984	T2983	advmod	e.g.,stages
R1699	T2985	T2983	punct	", ",stages
R17	T167	T165	prep	of,theme
R170	T330	T331	det	a,sheet
R1700	T2986	T2983	nmod	germ,stages
R1701	T2987	T2986	cc	and,germ
R1702	T2988	T2986	conj	peg,germ
R1703	T2989	T2983	punct	),stages
R1704	T2990	T2972	punct	", ",exhibited
R1705	T2991	T2972	nsubj	they,exhibited
R1706	T2992	T2993	det	no,signs
R1707	T2993	T2972	dobj	signs,exhibited
R1708	T2994	T2993	prep	of,signs
R1709	T2995	T2994	pobj	hybridization,of
R171	T331	T329	pobj	sheet,within
R1710	T2996	T2997	punct	(,1A
R1711	T2997	T2972	parataxis	1A,exhibited
R1712	T2998	T2997	compound	Figure,1A
R1713	T2999	T2997	punct	),1A
R1714	T3000	T2972	punct	.,exhibited
R1715	T3002	T3003	det	The,nature
R1716	T3003	T3005	nsubj	nature,was
R1717	T3004	T3003	amod	transient,nature
R1718	T3006	T3003	prep	of,nature
R1719	T3007	T3008	compound	Snail,expression
R172	T332	T326	auxpass	are,stimulated
R1720	T3008	T3006	pobj	expression,of
R1721	T3009	T3008	compound	mRNA,expression
R1722	T3010	T3008	prep	during,expression
R1723	T3011	T3012	compound	follicle,development
R1724	T3012	T3010	pobj	development,during
R1725	T3013	T3014	advmod	most,apparent
R1726	T3014	T3005	acomp	apparent,was
R1727	T3015	T3005	prep	in,was
R1728	T3016	T3017	amod	hybridized,sections
R1729	T3017	T3015	pobj	sections,in
R173	T333	T334	aux	to,undergo
R1730	T3018	T3017	compound	skin,sections
R1731	T3019	T3017	acl	containing,sections
R1732	T3020	T3019	dobj	follicles,containing
R1733	T3021	T3020	prep	from,follicles
R1734	T3022	T3023	nummod	two,waves
R1735	T3023	T3021	pobj	waves,from
R1736	T3024	T3023	amod	different,waves
R1737	T3025	T3023	prep	of,waves
R1738	T3026	T3025	pobj	morphogenesis,of
R1739	T3027	T3028	punct	(,shown
R174	T334	T326	advcl	undergo,stimulated
R1740	T3028	T3005	parataxis	shown,was
R1741	T3029	T3028	mark	as,shown
R1742	T3030	T3028	prep	in,shown
R1743	T3031	T3030	pobj	Figure,in
R1744	T3032	T3031	nummod	1,Figure
R1745	T3033	T3028	punct	),shown
R1746	T3034	T3005	punct	.,was
R1747	T3036	T3037	amod	Hybridizing,buds
R1748	T3037	T3039	nsubj	buds,appeared
R1749	T3038	T3037	compound	hair,buds
R175	T335	T336	amod	transcriptional,changes
R1750	T3040	T3037	prep	from,buds
R1751	T3041	T3042	det	a,wave
R1752	T3042	T3040	pobj	wave,from
R1753	T3043	T3042	amod	later,wave
R1754	T3044	T3039	oprd	juxtaposed,appeared
R1755	T3045	T3044	prep	with,juxtaposed
R1756	T3046	T3047	amod	nonhybridizing,follicles
R1757	T3047	T3045	pobj	follicles,with
R1758	T3048	T3047	prep	from,follicles
R1759	T3049	T3050	det	an,wave
R176	T336	T334	dobj	changes,undergo
R1760	T3050	T3048	pobj	wave,from
R1761	T3051	T3050	amod	earlier,wave
R1762	T3052	T3039	punct	.,appeared
R1763	T3054	T3055	aux	To,determine
R1764	T3055	T3056	advcl	determine,generated
R1765	T3057	T3058	mark	whether,reflected
R1766	T3058	T3055	ccomp	reflected,determine
R1767	T3059	T3060	det	this,nature
R1768	T3060	T3058	nsubjpass	nature,reflected
R1769	T3061	T3060	amod	transient,nature
R177	T337	T338	dep	that,result
R1770	T3062	T3060	prep	of,nature
R1771	T3063	T3064	compound	Snail,expression
R1772	T3064	T3062	pobj	expression,of
R1773	T3065	T3064	compound	mRNA,expression
R1774	T3066	T3058	auxpass	is,reflected
R1775	T3067	T3058	prep	at,reflected
R1776	T3068	T3069	det	the,level
R1777	T3069	T3067	pobj	level,at
R1778	T3070	T3069	compound	protein,level
R1779	T3071	T3056	punct	", ",generated
R178	T338	T336	relcl	result,changes
R1780	T3072	T3056	nsubj	we,generated
R1781	T3073	T3074	det	an,antibody
R1782	T3074	T3056	dobj	antibody,generated
R1783	T3075	T3074	prep	against,antibody
R1784	T3076	T3077	det	the,sequence
R1785	T3077	T3075	pobj	sequence,against
R1786	T3078	T3079	npadvmod	N,terminal
R1787	T3079	T3077	amod	terminal,sequence
R1788	T3080	T3079	punct	-,terminal
R1789	T3081	T3082	dep	that,resides
R179	T339	T338	prep	in,result
R1790	T3082	T3077	relcl	resides,sequence
R1791	T3083	T3082	advmod	upstream,resides
R1792	T3084	T3083	prep	of,upstream
R1793	T3085	T3086	det	the,domains
R1794	T3086	T3084	pobj	domains,of
R1795	T3087	T3088	advmod	more,conserved
R1796	T3088	T3086	amod	conserved,domains
R1797	T3089	T3090	compound	zinc,finger
R1798	T3090	T3086	compound	finger,domains
R1799	T3091	T3056	punct	.,generated
R18	T168	T167	pobj	organogenesis,of
R180	T340	T339	pobj	proliferation,in
R1800	T3093	T3094	mark	As,judged
R1801	T3094	T3095	advcl	judged,detect
R1802	T3096	T3094	prep	by,judged
R1803	T3097	T3098	compound	Western,blot
R1804	T3098	T3099	compound	blot,analysis
R1805	T3099	T3096	pobj	analysis,by
R1806	T3100	T3095	punct	", ",detect
R1807	T3101	T3102	det	the,antibody
R1808	T3102	T3095	nsubj	antibody,detect
R1809	T3103	T3095	aux	did,detect
R181	T341	T340	punct	", ",proliferation
R1810	T3104	T3095	neg	not,detect
R1811	T3105	T3106	amod	endogenous,proteins
R1812	T3106	T3095	dobj	proteins,detect
R1813	T3107	T3106	prep	from,proteins
R1814	T3108	T3109	amod	cultured,keratinocytes
R1815	T3109	T3107	pobj	keratinocytes,from
R1816	T3110	T3095	punct	", ",detect
R1817	T3111	T3095	cc	but,detect
R1818	T3112	T3113	nsubj	it,yield
R1819	T3113	T3095	conj	yield,detect
R182	T342	T343	amod	junctional,remodeling
R1820	T3114	T3113	aux	did,yield
R1821	T3115	T3116	det	a,band
R1822	T3116	T3113	dobj	band,yield
R1823	T3117	T3116	prep	of,band
R1824	T3118	T3119	det	the,size
R1825	T3119	T3117	pobj	size,of
R1826	T3120	T3119	amod	expected,size
R1827	T3121	T3113	prep	from,yield
R1828	T3122	T3121	pobj	keratinocytes,from
R1829	T3123	T3124	advmod	transiently,expressing
R183	T343	T340	conj	remodeling,proliferation
R1830	T3124	T3122	acl	expressing,keratinocytes
R1831	T3125	T3126	det	a,protein
R1832	T3126	T3124	dobj	protein,expressing
R1833	T3127	T3128	npadvmod	hemagglutinin,tagged
R1834	T3128	T3126	amod	tagged,protein
R1835	T3129	T3127	punct	(,hemagglutinin
R1836	T3130	T3127	appos	HA,hemagglutinin
R1837	T3131	T3128	punct	),tagged
R1838	T3132	T3128	punct	-,tagged
R1839	T3133	T3126	compound	Snail,protein
R184	T344	T343	punct	", ",remodeling
R1840	T3134	T3135	punct	(,1B
R1841	T3135	T3113	parataxis	1B,yield
R1842	T3136	T3135	compound	Figure,1B
R1843	T3137	T3135	punct	),1B
R1844	T3138	T3095	punct	.,detect
R1845	T3140	T3141	det	The,antibody
R1846	T3141	T3142	nsubj	antibody,recognized
R1847	T3143	T3142	advmod	also,recognized
R1848	T3144	T3145	det	a,band
R1849	T3145	T3142	dobj	band,recognized
R185	T345	T343	cc	and,remodeling
R1850	T3146	T3145	acl	corresponding,band
R1851	T3147	T3146	prep	to,corresponding
R1852	T3148	T3149	det	the,size
R1853	T3149	T3147	pobj	size,to
R1854	T3150	T3149	prep	of,size
R1855	T3151	T3152	amod	endogenous,Snail
R1856	T3152	T3150	pobj	Snail,of
R1857	T3153	T3149	punct	(,size
R1858	T3154	T3155	advmod	approximately,28
R1859	T3155	T3156	nummod	28,kDa
R186	T346	T347	compound	bud,formation
R1860	T3156	T3149	appos	kDa,size
R1861	T3157	T3149	punct	),size
R1862	T3158	T3149	prep	in,size
R1863	T3159	T3158	pobj	lysates,in
R1864	T3160	T3159	prep	from,lysates
R1865	T3161	T3162	amod	embryonic,skin
R1866	T3162	T3160	pobj	skin,from
R1867	T3163	T3162	compound	mouse,skin
R1868	T3164	T3145	punct	", ",band
R1869	T3165	T3166	det	the,appearance
R187	T347	T343	conj	formation,remodeling
R1870	T3166	T3168	dep	appearance,corresponded
R1871	T3167	T3166	amod	temporal,appearance
R1872	T3168	T3145	relcl	corresponded,band
R1873	T3169	T3166	prep	of,appearance
R1874	T3170	T3169	pobj	which,of
R1875	T3171	T3168	prep	to,corresponded
R1876	T3172	T3173	det	the,waves
R1877	T3173	T3171	pobj	waves,to
R1878	T3174	T3173	prep	of,waves
R1879	T3175	T3176	compound	hair,follicle
R188	T348	T322	punct	.,provide
R1880	T3176	T3177	compound	follicle,morphogenesis
R1881	T3177	T3174	pobj	morphogenesis,of
R1882	T3178	T3173	prep	from,waves
R1883	T3179	T3178	pobj	E15.5,from
R1884	T3180	T3178	prep	to,from
R1885	T3181	T3180	amod	newborn,to
R1886	T3182	T3183	advmod	when,formed
R1887	T3183	T3173	relcl	formed,waves
R1888	T3184	T3185	quantmod	over,90
R1889	T3185	T3186	nummod	90,%
R189	T350	T351	det	This,pathway
R1890	T3186	T3183	nsubjpass	%,formed
R1891	T3187	T3186	prep	of,%
R1892	T3188	T3189	det	the,hair
R1893	T3189	T3187	pobj	hair,of
R1894	T3190	T3189	prep	on,hair
R1895	T3191	T3192	det	the,mouse
R1896	T3192	T3190	pobj	mouse,on
R1897	T3193	T3183	auxpass	is,formed
R1898	T3194	T3195	punct	(,1B
R1899	T3195	T3142	parataxis	1B,recognized
R19	T169	T163	punct	", ",exchange
R190	T351	T354	nsubj	pathway,weaves
R1900	T3196	T3195	compound	Figure,1B
R1901	T3197	T3195	punct	),1B
R1902	T3198	T3142	punct	.,recognized
R1903	T3200	T3201	advcl	Consistent,detect
R1904	T3202	T3200	prep	with,Consistent
R1905	T3203	T3204	det	the,data
R1906	T3204	T3202	pobj	data,with
R1907	T3205	T3206	compound	Western,blot
R1908	T3206	T3204	compound	blot,data
R1909	T3207	T3201	punct	", ",detect
R191	T352	T351	amod	novel,pathway
R1910	T3208	T3209	amod	immunohistochemical,analysis
R1911	T3209	T3201	nsubj	analysis,detect
R1912	T3210	T3201	aux	did,detect
R1913	T3211	T3201	neg	not,detect
R1914	T3212	T3201	dobj	Snail,detect
R1915	T3213	T3201	prep	in,detect
R1916	T3214	T3215	amod	single,layered
R1917	T3215	T3217	amod	layered,epidermis
R1918	T3216	T3215	punct	-,layered
R1919	T3217	T3213	pobj	epidermis,in
R192	T353	T351	compound	signaling,pathway
R1920	T3218	T3217	compound	E13.5,epidermis
R1921	T3219	T3220	punct	(,1C
R1922	T3220	T3217	parataxis	1C,epidermis
R1923	T3221	T3220	compound	Figure,1C
R1924	T3222	T3220	punct	),1C
R1925	T3223	T3213	cc	nor,in
R1926	T3224	T3213	conj	in,in
R1927	T3225	T3226	det	the,placode
R1928	T3226	T3224	pobj	placode,in
R1929	T3227	T3201	punct	", ",detect
R193	T355	T354	advmod	further,weaves
R1930	T3228	T3229	dep	which,is
R1931	T3229	T3201	advcl	is,detect
R1932	T3230	T3231	det	the,sign
R1933	T3231	T3229	attr	sign,is
R1934	T3232	T3231	amod	earliest,sign
R1935	T3233	T3231	amod	morphological,sign
R1936	T3234	T3231	prep	of,sign
R1937	T3235	T3236	det	the,commitment
R1938	T3236	T3234	pobj	commitment,of
R1939	T3237	T3236	prep	of,commitment
R194	T356	T354	prt	together,weaves
R1940	T3238	T3239	amod	multipotent,cells
R1941	T3239	T3237	pobj	cells,of
R1942	T3240	T3239	prep	of,cells
R1943	T3241	T3242	det	the,ectoderm
R1944	T3242	T3240	pobj	ectoderm,of
R1945	T3243	T3242	amod	embryonic,ectoderm
R1946	T3244	T3236	prep	to,commitment
R1947	T3245	T3246	det	a,fate
R1948	T3246	T3244	pobj	fate,to
R1949	T3247	T3248	compound	hair,cell
R195	T357	T358	det	the,web
R1950	T3248	T3246	compound	cell,fate
R1951	T3249	T3250	punct	(,1D
R1952	T3250	T3201	parataxis	1D,detect
R1953	T3251	T3250	compound	Figure,1D
R1954	T3252	T3250	punct	),1D
R1955	T3253	T3201	punct	.,detect
R1956	T3255	T3256	advcl	Consistent,labeled
R1957	T3257	T3255	prep	with,Consistent
R1958	T3258	T3259	det	the,results
R1959	T3259	T3257	pobj	results,with
R196	T358	T354	dobj	web,weaves
R1960	T3260	T3261	advmod	in,situ
R1961	T3261	T3259	amod	situ,results
R1962	T3262	T3259	compound	hybridization,results
R1963	T3263	T3256	punct	", ",labeled
R1964	T3264	T3265	amod	anti-Snail,antibody
R1965	T3265	T3256	nsubj	antibody,labeled
R1966	T3266	T3267	advmod	only,buds
R1967	T3267	T3256	dobj	buds,labeled
R1968	T3268	T3267	compound	hair,buds
R1969	T3269	T3267	cc	and,buds
R197	T359	T358	prep	of,web
R1970	T3270	T3269	neg	not,and
R1971	T3271	T3267	conj	follicles,buds
R1972	T3272	T3256	prep	at,labeled
R1973	T3273	T3274	advmod	more,stages
R1974	T3274	T3272	pobj	stages,at
R1975	T3275	T3274	amod	mature,stages
R1976	T3276	T3274	prep	of,stages
R1977	T3277	T3276	pobj	development,of
R1978	T3278	T3279	punct	(,1E
R1979	T3279	T3256	parataxis	1E,labeled
R198	T360	T361	amod	different,morphogens
R1980	T3280	T3279	compound	Figure,1E
R1981	T3281	T3279	punct	),1E
R1982	T3282	T3256	punct	.,labeled
R1983	T3284	T3285	advcl	Taken,appeared
R1984	T3286	T3284	advmod	together,Taken
R1985	T3287	T3285	punct	", ",appeared
R1986	T3288	T3289	det	the,antibody
R1987	T3289	T3285	nsubj	antibody,appeared
R1988	T3290	T3289	amod	anti-Snail,antibody
R1989	T3291	T3292	aux	to,be
R199	T361	T359	pobj	morphogens,of
R1990	T3292	T3285	xcomp	be,appeared
R1991	T3293	T3292	acomp	specific,be
R1992	T3294	T3293	prep	for,specific
R1993	T3295	T3296	poss	its,protein
R1994	T3296	T3294	pobj	protein,for
R1995	T3297	T3296	compound	target,protein
R1996	T3298	T3285	punct	.,appeared
R1997	T3300	T3301	nsubj	It,detect
R1998	T3302	T3301	aux	did,detect
R1999	T3303	T3301	neg	not,detect
R2	T149	T147	compound	Signaling,Pathway
R20	T170	T171	amod	certain,cells
R200	T362	T361	cc	and,morphogens
R2000	T3304	T3305	amod	other,members
R2001	T3305	T3301	dobj	members,detect
R2002	T3306	T3305	compound	Snail,members
R2003	T3307	T3305	compound	family,members
R2004	T3308	T3305	acl	known,members
R2005	T3309	T3310	aux	to,expressed
R2006	T3310	T3308	xcomp	expressed,known
R2007	T3311	T3310	auxpass	be,expressed
R2008	T3312	T3310	prep	in,expressed
R2009	T3313	T3312	pobj	keratinocytes,in
R201	T363	T364	amod	downstream,events
R2010	T3314	T3313	cc	and,keratinocytes
R2011	T3315	T3314	punct	/,and
R2012	T3316	T3314	cc	or,and
R2013	T3317	T3313	conj	skin,keratinocytes
R2014	T3318	T3319	punct	(,data
R2015	T3319	T3308	meta	data,known
R2016	T3320	T3319	amod	unpublished,data
R2017	T3321	T3319	punct	),data
R2018	T3322	T3301	punct	.,detect
R2019	T3324	T3325	advmod	Furthermore,paralleled
R202	T364	T361	conj	events,morphogens
R2020	T3326	T3325	punct	", ",paralleled
R2021	T3327	T3328	det	the,data
R2022	T3328	T3325	nsubj	data,paralleled
R2023	T3329	T3328	amod	immunohistochemical,data
R2024	T3330	T3331	poss	our,data
R2025	T3331	T3325	dobj	data,paralleled
R2026	T3332	T3333	nmod	Snail,hybridization
R2027	T3333	T3331	compound	hybridization,data
R2028	T3334	T3335	advmod	in,situ
R2029	T3335	T3333	amod	situ,hybridization
R203	T365	T364	amod	transcriptional,events
R2030	T3336	T3325	advcl	revealing,paralleled
R2031	T3337	T3338	amod	transient,expression
R2032	T3338	T3336	dobj	expression,revealing
R2033	T3339	T3338	compound	Snail,expression
R2034	T3340	T3336	prep	at,revealing
R2035	T3341	T3342	det	the,stage
R2036	T3342	T3340	pobj	stage,at
R2037	T3343	T3344	compound	hair,bud
R2038	T3344	T3342	compound	bud,stage
R2039	T3345	T3346	punct	(,1A
R204	T366	T367	dep	that,guide
R2040	T3346	T3325	parataxis	1A,paralleled
R2041	T3347	T3346	compound	Figure,1A
R2042	T3348	T3346	punct	),1A
R2043	T3349	T3325	punct	.,paralleled
R2044	T3351	T3352	mark	As,judged
R2045	T3352	T3353	advcl	judged,localized
R2046	T3354	T3352	prep	by,judged
R2047	T3355	T3354	pobj	immunohistochemistry,by
R2048	T3356	T3353	punct	", ",localized
R2049	T3357	T3358	compound	Snail,protein
R205	T367	T358	relcl	guide,web
R2050	T3358	T3353	nsubjpass	protein,localized
R2051	T3359	T3353	auxpass	was,localized
R2052	T3360	T3353	prep	to,localized
R2053	T3361	T3362	det	the,nuclei
R2054	T3362	T3360	pobj	nuclei,to
R2055	T3363	T3362	prep	of,nuclei
R2056	T3364	T3365	det	the,cells
R2057	T3365	T3363	pobj	cells,of
R2058	T3366	T3367	compound	hair,bud
R2059	T3367	T3365	compound	bud,cells
R206	T368	T369	compound	hair,bud
R2060	T3368	T3369	punct	(,1E
R2061	T3369	T3353	parataxis	1E,localized
R2062	T3370	T3369	compound	Figure,1E
R2063	T3371	T3369	punct	),1E
R2064	T3372	T3353	punct	.,localized
R2065	T3374	T3375	det	This,feature
R2066	T3375	T3376	nsubj	feature,was
R2067	T3377	T3376	acomp	consistent,was
R2068	T3378	T3377	prep	with,consistent
R2069	T3379	T3380	poss	Snail,function
R207	T369	T370	compound	bud,formation
R2070	T3380	T3378	pobj	function,with
R2071	T3381	T3379	case	's,Snail
R2072	T3382	T3380	amod	known,function
R2073	T3383	T3380	prep	as,function
R2074	T3384	T3385	det	a,repressor
R2075	T3385	T3383	pobj	repressor,as
R2076	T3386	T3385	amod	transcriptional,repressor
R2077	T3387	T3388	punct	[,13
R2078	T3388	T3376	parataxis	13,was
R2079	T3389	T3388	nummod	12,13
R208	T370	T367	dobj	formation,guide
R2080	T3390	T3388	punct	",",13
R2081	T3391	T3388	punct	],13
R2082	T3392	T3376	punct	.,was
R2083	T3394	T3395	advmod	Additionally,detected
R2084	T3396	T3395	punct	", ",detected
R2085	T3397	T3398	amod	anti-Snail,labeling
R2086	T3398	T3395	nsubjpass	labeling,detected
R2087	T3399	T3395	auxpass	was,detected
R2088	T3400	T3395	prep	in,detected
R2089	T3401	T3402	advmod	only,four
R209	T371	T367	prep	within,guide
R2090	T3402	T3406	nummod	four,waves
R2091	T3403	T3402	quantmod	three,four
R2092	T3404	T3402	quantmod	of,four
R2093	T3405	T3402	quantmod	the,four
R2094	T3406	T3400	pobj	waves,in
R2095	T3407	T3406	amod	major,waves
R2096	T3408	T3406	prep	of,waves
R2097	T3409	T3410	compound	follicle,morphogenesis
R2098	T3410	T3408	pobj	morphogenesis,of
R2099	T3411	T3395	punct	.,detected
R21	T171	T163	nsubj	cells,exchange
R210	T372	T373	det	the,skin
R2100	T3413	T3414	nsubjpass	Snail,found
R2101	T3415	T3414	auxpass	was,found
R2102	T3416	T3414	neg	not,found
R2103	T3417	T3414	prep	in,found
R2104	T3418	T3419	det	the,buds
R2105	T3419	T3417	pobj	buds,in
R2106	T3420	T3419	prep	of,buds
R2107	T3421	T3422	compound	guard,hairs
R2108	T3422	T3420	pobj	hairs,of
R2109	T3423	T3424	dep	that,are
R211	T373	T371	pobj	skin,within
R2110	T3424	T3422	advcl	are,hairs
R2111	T3425	T3426	det	the,earliest
R2112	T3426	T3424	attr	earliest,are
R2113	T3427	T3426	prep	of,earliest
R2114	T3428	T3429	det	all,hairs
R2115	T3429	T3427	pobj	hairs,of
R2116	T3430	T3431	aux	to,form
R2117	T3431	T3429	advcl	form,hairs
R2118	T3432	T3433	punct	(,at
R2119	T3433	T3426	parataxis	at,earliest
R212	T374	T373	amod	developing,skin
R2120	T3434	T3433	pobj	E13.5,at
R2121	T3435	T3433	punct	),at
R2122	T3436	T3424	punct	", ",are
R2123	T3437	T3424	cc	and,are
R2124	T3438	T3439	dep	which,constitute
R2125	T3439	T3424	conj	constitute,are
R2126	T3440	T3441	amod	less,5
R2127	T3441	T3443	nummod	5,%
R2128	T3442	T3441	quantmod	than,5
R2129	T3443	T3439	dobj	%,constitute
R213	T375	T354	punct	.,weaves
R2130	T3444	T3443	prep	of,%
R2131	T3445	T3446	det	the,coat
R2132	T3446	T3444	pobj	coat,of
R2133	T3447	T3446	compound	mouse,coat
R2134	T3448	T3449	punct	(,data
R2135	T3449	T3414	meta	data,found
R2136	T3450	T3449	amod	unpublished,data
R2137	T3451	T3449	punct	),data
R2138	T3452	T3414	punct	.,found
R2139	T3454	T3455	mark	As,judged
R214	T146	T147	det	A,Pathway
R2140	T3455	T3456	advcl	judged,coincided
R2141	T3457	T3455	prep	by,judged
R2142	T3458	T3457	pobj	immunofluorescence,by
R2143	T3459	T3458	prep	with,immunofluorescence
R2144	T3460	T3459	pobj	antibodies,with
R2145	T3461	T3460	prep	against,antibodies
R2146	T3462	T3463	det	the,antigen
R2147	T3463	T3461	pobj	antigen,against
R2148	T3464	T3463	amod	proliferating,antigen
R2149	T3465	T3463	amod	nuclear,antigen
R2150	T3466	T3463	appos	Ki67,antigen
R2151	T3467	T3456	punct	", ",coincided
R2152	T3468	T3469	det	the,timing
R2153	T3469	T3456	nsubj	timing,coincided
R2154	T3470	T3469	prep	of,timing
R2155	T3471	T3472	compound	Snail,expression
R2156	T3472	T3470	pobj	expression,of
R2157	T3473	T3456	prep	with,coincided
R2158	T3474	T3475	det	the,stage
R2159	T3475	T3473	pobj	stage,with
R2160	T3476	T3477	prep	at,enhanced
R2161	T3477	T3475	relcl	enhanced,stage
R2162	T3478	T3476	pobj	which,at
R2163	T3479	T3480	det	the,follicle
R2164	T3480	T3477	nsubj	follicle,enhanced
R2165	T3481	T3480	amod	developing,follicle
R2166	T3482	T3483	poss	its,proliferation
R2167	T3483	T3477	dobj	proliferation,enhanced
R2168	T3484	T3483	cc	and,proliferation
R2169	T3485	T3486	amod	down,growth
R2170	T3486	T3483	conj	growth,proliferation
R2171	T3487	T3486	punct	-,growth
R2172	T3488	T3489	punct	(,1F
R2173	T3489	T3456	parataxis	1F,coincided
R2174	T3490	T3489	compound	Figure,1F
R2175	T3491	T3489	punct	),1F
R2176	T3492	T3456	punct	.,coincided
R2177	T3494	T3495	nsubj	Immunohistochemistry,marked
R2178	T3496	T3494	prep	with,Immunohistochemistry
R2179	T3497	T3496	pobj	antibodies,with
R2180	T3498	T3497	prep	against,antibodies
R2181	T3499	T3500	det	the,form
R2182	T3500	T3498	pobj	form,against
R2183	T3501	T3500	amod	active,form
R2184	T3502	T3500	punct	(,form
R2185	T3503	T3500	amod	phosphorylated,form
R2186	T3504	T3500	punct	),form
R2187	T3505	T3500	prep	of,form
R2188	T3506	T3505	pobj	MAPK,of
R2189	T3507	T3500	punct	(,form
R2190	T3508	T3500	appos	pMAPK,form
R2191	T3509	T3495	punct	),marked
R2192	T3510	T3511	det	a,subset
R2193	T3511	T3495	dobj	subset,marked
R2194	T3512	T3511	prep	of,subset
R2195	T3513	T3514	det	the,cells
R2196	T3514	T3512	pobj	cells,of
R2197	T3601	T3599	punct	;,1H
R2198	T3515	T3514	amod	proliferating,cells
R2199	T3602	T3599	advcl	see,1H
R22	T172	T171	prep	within,cells
R2200	T3516	T3514	punct	(,cells
R2201	T3603	T3602	advmod	also,see
R2202	T3604	T3602	punct	[,see
R2203	T3517	T3518	npadvmod	Ki67,positive
R2204	T3605	T3602	dobj	4,see
R2205	T3606	T3599	punct	],1H
R2206	T3607	T3599	punct	),1H
R2207	T3518	T3514	amod	positive,cells
R2208	T3608	T3583	punct	.,exhibited
R2209	T3519	T3518	punct	-,positive
R2210	T3520	T3514	punct	),cells
R2211	T3521	T3495	punct	", ",marked
R2212	T3522	T3495	cc	and,marked
R2213	T3523	T3524	npadvmod	pMAPK,positive
R2214	T3524	T3526	amod	positive,cells
R2215	T3525	T3524	punct	-,positive
R2216	T3526	T3527	nsubjpass	cells,enriched
R2217	T3527	T3495	conj	enriched,marked
R2218	T3528	T3527	auxpass	were,enriched
R2219	T3529	T3527	prep	in,enriched
R2220	T3530	T3531	det	the,bud
R2221	T3531	T3529	pobj	bud,in
R2222	T3532	T3531	compound	hair,bud
R2223	T3533	T3534	punct	(,1G
R2224	T3534	T3527	parataxis	1G,enriched
R2225	T3535	T3534	compound	Figure,1G
R2226	T3536	T3534	punct	),1G
R2227	T3537	T3527	punct	.,enriched
R2228	T3539	T3540	det	The,timing
R2229	T3540	T3541	nsubj	timing,appeared
R2230	T3542	T3540	prep	of,timing
R2231	T3543	T3544	compound	Snail,induction
R2232	T3544	T3542	pobj	induction,of
R2233	T3545	T3544	cc	and,induction
R2234	T3546	T3547	nmod	Ki67,enrichment
R2235	T3547	T3544	conj	enrichment,induction
R2236	T3548	T3546	cc	and,Ki67
R2237	T3549	T3546	conj	pMAPK,Ki67
R2238	T3550	T3540	prep	in,timing
R2239	T3551	T3552	det	the,bud
R2240	T3552	T3550	pobj	bud,in
R2241	T3553	T3552	compound	hair,bud
R2242	T3554	T3555	aux	to,follow
R2243	T3555	T3541	xcomp	follow,appeared
R2244	T3556	T3555	advmod	closely,follow
R2245	T3557	T3558	det	the,induction
R2246	T3558	T3555	dobj	induction,follow
R2247	T3559	T3558	prep	of,induction
R2248	T3560	T3561	nmod	LEF,activity
R2249	T3561	T3559	pobj	activity,of
R2250	T3562	T3560	punct	-,LEF
R2251	T3563	T3560	nummod	1,LEF
R2252	T3564	T3560	punct	/,LEF
R2253	T3565	T3566	compound	β,catenin
R2254	T3566	T3560	appos	catenin,LEF
R2255	T3567	T3566	punct	-,catenin
R2256	T3568	T3561	punct	", ",activity
R2257	T3569	T3561	acl	known,activity
R2258	T3570	T3571	aux	to,initiate
R2259	T3571	T3569	xcomp	initiate,known
R2260	T3572	T3571	prep	in,initiate
R2261	T3573	T3574	det	the,stage
R2262	T3574	T3572	pobj	stage,in
R2263	T3575	T3576	compound	hair,placode
R2264	T3576	T3574	compound	placode,stage
R2265	T3577	T3578	punct	[,20
R2266	T3578	T3555	parataxis	20,follow
R2267	T3579	T3578	punct	],20
R2268	T3580	T3541	punct	.,appeared
R2269	T3582	T3583	advmod	However,exhibited
R227	T1019	T1020	amod	Mammalian,development
R2270	T3584	T3583	punct	", ",exhibited
R2271	T3585	T3583	prep	like,exhibited
R2272	T3586	T3585	pobj	placodes,like
R2273	T3587	T3583	punct	", ",exhibited
R2274	T3588	T3589	compound	hair,buds
R2275	T3589	T3583	nsubj	buds,exhibited
R2276	T3590	T3591	amod	down,regulation
R2277	T3591	T3583	dobj	regulation,exhibited
R2278	T3592	T3591	punct	-,regulation
R2279	T3593	T3591	prep	in,regulation
R228	T1020	T1021	nsubj	development,involves
R2280	T3594	T3595	compound	E,cadherin
R2281	T3595	T3597	compound	cadherin,expression
R2282	T3596	T3595	punct	-,cadherin
R2283	T3597	T3593	pobj	expression,in
R2284	T3598	T3599	punct	(,1H
R2285	T3599	T3583	parataxis	1H,exhibited
R2286	T3600	T3599	compound	Figure,1H
R2289	T3910	T3911	amod	Sustained,Expression
R229	T1022	T1023	det	the,morphogenesis
R2290	T3911	T3912	nsubj	Expression,Results
R2291	T3913	T3911	prep	of,Expression
R2292	T3914	T3913	pobj	Snail,of
R2293	T3915	T3912	prep	in,Results
R2294	T3916	T3917	amod	Epidermal,Hyperproliferation
R2295	T3917	T3915	pobj	Hyperproliferation,in
R2296	T3918	T3917	cc	and,Hyperproliferation
R2297	T3919	T3920	compound	Differentiation,Defects
R2298	T3920	T3917	conj	Defects,Hyperproliferation
R2299	T3921	T3912	prep	in,Results
R23	T173	T174	det	a,sheet
R230	T1023	T1021	dobj	morphogenesis,involves
R2300	T3922	T3923	compound	Tg,Skin
R2301	T3923	T3921	pobj	Skin,in
R2302	T3924	T3923	compound	Mouse,Skin
R2303	T3926	T3927	det	The,spike
R2304	T3927	T3929	nsubj	spike,prompted
R2305	T3928	T3927	amod	striking,spike
R2306	T3930	T3927	prep	of,spike
R2307	T3931	T3932	compound	Snail,expression
R2308	T3932	T3930	pobj	expression,of
R2309	T3933	T3927	amod	coincident,spike
R231	T1024	T1023	prep	of,morphogenesis
R2310	T3934	T3933	prep	with,coincident
R2311	T3935	T3936	compound	hair,bud
R2312	T3936	T3937	compound	bud,formation
R2313	T3937	T3934	pobj	formation,with
R2314	T3938	T3937	cc	and,formation
R2315	T3939	T3940	amod	enhanced,proliferation
R2316	T3940	T3937	conj	proliferation,formation
R2317	T3941	T3929	dobj	us,prompted
R2318	T3942	T3943	aux	to,examine
R2319	T3943	T3929	xcomp	examine,prompted
R232	T1025	T1026	amod	complex,structures
R2320	T3944	T3945	det	the,consequences
R2321	T3945	T3943	dobj	consequences,examine
R2322	T3946	T3945	prep	of,consequences
R2323	T3947	T3948	advmod	ectopically,expressing
R2324	T3948	T3946	pcomp	expressing,of
R2325	T3949	T3948	dobj	Snail,expressing
R2326	T3950	T3948	advmod	elsewhere,expressing
R2327	T3951	T3950	prep	in,elsewhere
R2328	T3952	T3953	compound	mouse,epidermis
R2329	T3953	T3951	pobj	epidermis,in
R233	T1026	T1024	pobj	structures,of
R2330	T3954	T3953	compound	skin,epidermis
R2331	T3955	T3929	punct	.,prompted
R2332	T3957	T3958	aux	To,distinguish
R2333	T3958	T3959	advcl	distinguish,used
R2334	T3960	T3958	dobj	Tg,distinguish
R2335	T3961	T3958	prep	from,distinguish
R2336	T3962	T3963	amod	endogenous,Snail
R2337	T3963	T3961	pobj	Snail,from
R2338	T3964	T3959	punct	", ",used
R2339	T3965	T3959	nsubj	we,used
R234	T1027	T1028	advmod	three,dimensional
R2340	T3966	T3967	det	the,epitope
R2341	T3967	T3959	dobj	epitope,used
R2342	T3968	T3967	compound	HA,epitope
R2343	T3969	T3967	punct	-,epitope
R2344	T3970	T3967	punct	", ",epitope
R2345	T3971	T3967	acl	shown,epitope
R2346	T3972	T3971	advmod	previously,shown
R2347	T3973	T3974	neg	not,alter
R2348	T3974	T3971	xcomp	alter,shown
R2349	T3975	T3974	aux	to,alter
R235	T1028	T1026	amod	dimensional,structures
R2350	T3976	T3977	poss	Snail,activity
R2351	T3977	T3974	dobj	activity,alter
R2352	T3978	T3976	case	's,Snail
R2353	T3979	T3977	amod	transcriptional,activity
R2354	T3980	T3981	punct	[,12
R2355	T3981	T3959	parataxis	12,used
R2356	T3982	T3981	punct	],12
R2357	T3983	T3959	punct	.,used
R2358	T3985	T3986	prep	Of,expressed
R2359	T3987	T3988	nummod	20,animals
R236	T1029	T1028	punct	-,dimensional
R2360	T3988	T3985	pobj	animals,Of
R2361	T3989	T3990	nmod	K14,Snail
R2362	T3990	T3988	nmod	Snail,animals
R2363	T3991	T3990	punct	-,Snail
R2364	T3992	T3988	punct	[,animals
R2365	T3993	T3988	nmod	HA,animals
R2366	T3994	T3988	punct	],animals
R2367	T3995	T3988	compound	Tg,animals
R2368	T3996	T3988	acl	generated,animals
R2369	T3997	T3986	punct	", ",expressed
R237	T1030	T1023	prep	from,morphogenesis
R2370	T3998	T3986	nsubj	three,expressed
R2371	T3999	T4000	det	the,transgene
R2372	T4000	T3986	dobj	transgene,expressed
R2373	T4001	T3986	cc	and,expressed
R2374	T4002	T4003	nsubj	all,exhibited
R2375	T4003	T3986	conj	exhibited,expressed
R2376	T4004	T4005	amod	analogous,phenotypes
R2377	T4005	T4003	dobj	phenotypes,exhibited
R2378	T4006	T3986	punct	.,expressed
R2379	T4008	T4009	nsubj	Mice,died
R238	T1031	T1032	advmod	seemingly,uniform
R2380	T4010	T4011	dep	that,integrated
R2381	T4011	T4008	relcl	integrated,Mice
R2382	T4012	T4013	det	the,transgene
R2383	T4013	T4011	dobj	transgene,integrated
R2384	T4014	T4011	prep	at,integrated
R2385	T4015	T4016	det	the,stage
R2386	T4016	T4014	pobj	stage,at
R2387	T4017	T4018	amod	single,cell
R2388	T4018	T4016	compound	cell,stage
R2389	T4019	T4018	punct	-,cell
R239	T1032	T1033	amod	uniform,sheets
R2390	T4020	T4009	prep	at,died
R2391	T4021	T4020	cc	or,at
R2392	T4022	T4023	advmod	shortly,after
R2393	T4023	T4020	conj	after,at
R2394	T4024	T4023	pobj	birth,after
R2395	T4025	T4009	punct	.,died
R2396	T4027	T4028	det	The,mice
R2397	T4028	T4035	nsubj	mice,harbored
R2398	T4029	T4028	nummod	three,mice
R2399	T4030	T4028	amod	surviving,mice
R24	T174	T172	pobj	sheet,within
R240	T1033	T1030	pobj	sheets,from
R2400	T4031	T4032	amod	full,Tg
R2401	T4032	T4028	compound	Tg,mice
R2402	T4033	T4032	punct	-,Tg
R2403	T4034	T4028	compound	founder,mice
R2404	T4036	T4037	compound	transgene,integrations
R2405	T4037	T4035	dobj	integrations,harbored
R2406	T4038	T4039	dep	that,gave
R2407	T4039	T4037	relcl	gave,integrations
R2408	T4040	T4041	amod	stable,transmission
R2409	T4041	T4039	dobj	transmission,gave
R241	T1034	T1033	cc	or,sheets
R2410	T4042	T4041	prep	of,transmission
R2411	T4043	T4044	amod	mosaic,gene
R2412	T4044	T4046	compound	gene,expression
R2413	T4045	T4044	compound	Snail,gene
R2414	T4046	T4042	pobj	expression,of
R2415	T4047	T4039	prep	through,gave
R2416	T4048	T4049	det	the,germline
R2417	T4049	T4047	pobj	germline,through
R2418	T4050	T4035	punct	.,harbored
R2419	T4052	T4053	advmod	Progressively,poor
R242	T1035	T1033	conj	masses,sheets
R2420	T4053	T4054	amod	poor,health
R2421	T4054	T4055	nsubj	health,necessitated
R2422	T4056	T4057	nsubj	our,sacrificing
R2423	T4057	T4055	ccomp	sacrificing,necessitated
R2424	T4058	T4059	amod	most,offspring
R2425	T4059	T4057	dobj	offspring,sacrificing
R2426	T4060	T4059	prep	from,offspring
R2427	T4061	T4062	det	these,lines
R2428	T4062	T4060	pobj	lines,from
R2429	T4063	T4057	prep	within,sacrificing
R243	T1036	T1033	prep	of,sheets
R2430	T4064	T4065	det	a,year
R2431	T4065	T4063	pobj	year,within
R2432	T4066	T4065	prep	of,year
R2433	T4067	T4066	pobj	birth,of
R2434	T4068	T4055	punct	.,necessitated
R2435	T4070	T4071	mark	As,grew
R2436	T4071	T4075	advcl	grew,became
R2437	T4072	T4073	compound	Snail,animals
R2438	T4073	T4071	nsubj	animals,grew
R2439	T4074	T4073	compound	Tg,animals
R244	T1037	T1036	pobj	cells,of
R2440	T4076	T4075	punct	", ",became
R2441	T4077	T4075	nsubj	they,became
R2442	T4078	T4075	acomp	distinguished,became
R2443	T4079	T4078	prep	by,distinguished
R2444	T4080	T4081	poss	their,size
R2445	T4081	T4079	pobj	size,by
R2446	T4082	T4081	amod	small,size
R2447	T4083	T4081	punct	", ",size
R2448	T4084	T4085	amod	short,tails
R2449	T4085	T4081	conj	tails,size
R245	T1038	T1021	punct	.,involves
R2450	T4086	T4085	punct	", ",tails
R2451	T4087	T4085	cc	and,tails
R2452	T4088	T4089	amod	flaky,skin
R2453	T4089	T4085	conj	skin,tails
R2454	T4090	T4091	punct	(,2A
R2455	T4091	T4075	parataxis	2A,became
R2456	T4092	T4091	compound	Figure,2A
R2457	T4093	T4091	punct	),2A
R2458	T4094	T4075	punct	.,became
R2459	T4096	T4097	amod	Histological,analyses
R246	T1040	T1041	det	A,structure
R2460	T4097	T4098	nsubj	analyses,revealed
R2461	T4099	T4097	prep	of,analyses
R2462	T4100	T4101	nummod	3,d
R2463	T4101	T4103	npadvmod	d,old
R2464	T4102	T4101	punct	-,d
R2465	T4103	T4104	amod	old,mice
R2466	T4104	T4099	pobj	mice,of
R2467	T4105	T4106	punct	(,P3
R2468	T4106	T4103	parataxis	P3,old
R2469	T4107	T4106	punct	),P3
R247	T1041	T1046	nsubj	structure,initiates
R2470	T4108	T4109	compound	mosaic,patches
R2471	T4109	T4098	dobj	patches,revealed
R2472	T4110	T4109	acl	marked,patches
R2473	T4111	T4110	agent	by,marked
R2474	T4112	T4113	amod	epidermal,thickening
R2475	T4113	T4111	pobj	thickening,by
R2476	T4114	T4115	punct	(,2B
R2477	T4115	T4098	parataxis	2B,revealed
R2478	T4116	T4115	compound	Figure,2B
R2479	T4117	T4115	punct	),2B
R248	T1042	T1041	amod	simple,structure
R2480	T4118	T4098	punct	.,revealed
R2481	T4120	T4121	det	The,morphology
R2482	T4121	T4123	nsubjpass	morphology,reflected
R2483	T4122	T4121	compound	mosaic,morphology
R2484	T4124	T4123	auxpass	was,reflected
R2485	T4125	T4123	prep	at,reflected
R2486	T4126	T4127	det	the,level
R2487	T4127	T4125	pobj	level,at
R2488	T4128	T4127	prep	of,level
R2489	T4129	T4130	compound	Tg,protein
R249	T1043	T1044	npadvmod	bud,like
R2490	T4130	T4128	pobj	protein,of
R2491	T4131	T4130	compound	Snail,protein
R2492	T4132	T4123	punct	", ",reflected
R2493	T4133	T4134	mark	with,expressing
R2494	T4134	T4123	advcl	expressing,reflected
R2495	T4135	T4136	advmod	only,regions
R2496	T4136	T4134	nsubj	regions,expressing
R2497	T4137	T4136	det	the,regions
R2498	T4138	T4136	amod	hyperthickened,regions
R2499	T4139	T4140	amod	nuclear,Snail
R25	T175	T174	amod	multipotent,sheet
R250	T1044	T1041	amod	like,structure
R2500	T4140	T4134	dobj	Snail,expressing
R2501	T4141	T4142	npadvmod	HA,tagged
R2502	T4142	T4140	amod	tagged,Snail
R2503	T4143	T4142	punct	-,tagged
R2504	T4144	T4145	punct	(,2C
R2505	T4145	T4123	parataxis	2C,reflected
R2506	T4146	T4145	compound	Figure,2C
R2507	T4147	T4145	punct	),2C
R2508	T4148	T4123	punct	.,reflected
R2509	T4150	T4151	det	These,areas
R251	T1045	T1044	punct	-,like
R2510	T4151	T4153	nsubjpass	areas,marked
R2511	T4152	T4151	amod	hyperthickened,areas
R2512	T4154	T4153	auxpass	were,marked
R2513	T4155	T4153	agent	by,marked
R2514	T4156	T4157	amod	excessive,proliferation
R2515	T4157	T4155	pobj	proliferation,by
R2516	T4158	T4153	punct	", ",marked
R2517	T4159	T4160	mark	as,revealed
R2518	T4160	T4153	advcl	revealed,marked
R2519	T4161	T4160	agent	by,revealed
R252	T1047	T1048	det	the,formation
R2520	T4162	T4161	pobj	antibodies,by
R2521	T4163	T4162	prep	against,antibodies
R2522	T4164	T4165	det	the,antigen
R2523	T4165	T4163	pobj	antigen,against
R2524	T4166	T4165	amod	proliferating,antigen
R2525	T4167	T4165	amod	nuclear,antigen
R2526	T4168	T4165	appos	Ki67,antigen
R2527	T4169	T4170	punct	(,2D
R2528	T4170	T4153	parataxis	2D,marked
R2529	T4171	T4170	compound	Figure,2D
R253	T1048	T1046	dobj	formation,initiates
R2530	T4172	T4170	cc	and,2D
R2531	T4173	T4170	conj	2E,2D
R2532	T4174	T4170	punct	),2D
R2533	T4175	T4153	punct	.,marked
R2534	T4177	T4178	amod	Activated,cells
R2535	T4178	T4183	nsubj	cells,were
R2536	T4179	T4178	punct	", ",cells
R2537	T4180	T4181	npadvmod	pMAPK,positive
R2538	T4181	T4178	amod	positive,cells
R2539	T4182	T4181	punct	-,positive
R254	T1049	T1048	prep	of,formation
R2540	T4184	T4183	advmod	also,were
R2541	T4185	T4183	acomp	prevalent,were
R2542	T4186	T4183	prep	in,were
R2543	T4187	T4188	det	these,areas
R2544	T4188	T4186	pobj	areas,in
R2545	T4189	T4190	punct	(,2F
R2546	T4190	T4183	parataxis	2F,were
R2547	T4191	T4190	compound	Figure,2F
R2548	T4192	T4190	cc	and,2F
R2549	T4193	T4190	conj	2G,2F
R255	T1050	T1051	amod	many,organs
R2550	T4194	T4190	punct	),2F
R2551	T4195	T4183	punct	", ",were
R2552	T4196	T4197	mark	as,were
R2553	T4197	T4183	advcl	were,were
R2554	T4198	T4197	nsubj	cells,were
R2555	T4199	T4198	acl	expressing,cells
R2556	T4200	T4199	dobj	keratin,expressing
R2557	T4201	T4200	nummod	6,keratin
R2558	T4202	T4200	punct	", ",keratin
R2559	T4203	T4204	det	a,keratin
R256	T1051	T1049	pobj	organs,of
R2560	T4204	T4200	appos	keratin,keratin
R2561	T4205	T4204	acl	induced,keratin
R2562	T4206	T4205	prep	in,induced
R2563	T4207	T4208	det	the,layers
R2564	T4208	T4206	pobj	layers,in
R2565	T4209	T4208	amod	suprabasal,layers
R2566	T4210	T4208	prep	of,layers
R2567	T4211	T4212	amod	hyperproliferative,skin
R2568	T4212	T4210	pobj	skin,of
R2569	T4213	T4214	punct	(,2H
R257	T1052	T1051	punct	", ",organs
R2570	T4214	T4183	parataxis	2H,were
R2571	T4215	T4214	compound	Figure,2H
R2572	T4216	T4214	cc	and,2H
R2573	T4217	T4214	conj	2I,2H
R2574	T4218	T4214	punct	),2H
R2575	T4219	T4183	punct	.,were
R2576	T4221	T4222	nsubj	Expression,block
R2577	T4223	T4221	prep	of,Expression
R2578	T4224	T4225	det	the,transgene
R2579	T4225	T4223	pobj	transgene,of
R258	T1053	T1051	prep	including,organs
R2580	T4226	T4225	compound	Snail,transgene
R2581	T4227	T4222	aux	did,block
R2582	T4228	T4222	neg	not,block
R2583	T4229	T4230	amod	terminal,differentiation
R2584	T4230	T4222	dobj	differentiation,block
R2585	T4231	T4222	prep	in,block
R2586	T4232	T4233	det	the,epidermis
R2587	T4233	T4231	pobj	epidermis,in
R2588	T4234	T4233	amod	hyperproliferative,epidermis
R2589	T4235	T4222	punct	", ",block
R259	T1054	T1053	pobj	lungs,including
R2590	T4236	T4222	cc	but,block
R2591	T4237	T4238	nsubj	it,distorted
R2592	T4238	T4222	conj	distorted,block
R2593	T4239	T4238	dobj	it,distorted
R2594	T4240	T4238	advmod	markedly,distorted
R2595	T4241	T4242	punct	(,3A
R2596	T4242	T4238	parataxis	3A,distorted
R2597	T4243	T4242	compound	Figure,3A
R2598	T4244	T4245	punct	–,3H
R2599	T4245	T4242	prep	3H,3A
R26	T176	T174	amod	epithelial,sheet
R260	T1055	T1054	punct	", ",lungs
R2600	T4246	T4242	punct	),3A
R2601	T4247	T4238	punct	.,distorted
R2602	T4249	T4250	advcl	Typical,led
R2603	T4251	T4249	prep	of,Typical
R2604	T4252	T4253	amod	most,conditions
R2605	T4253	T4251	pobj	conditions,of
R2606	T4254	T4253	compound	hyperproliferating,conditions
R2607	T4255	T4250	punct	", ",led
R2608	T4256	T4257	compound	Snail,expression
R2609	T4257	T4250	nsubj	expression,led
R261	T1056	T1057	amod	spinal,cord
R2610	T4258	T4250	prep	to,led
R2611	T4259	T4260	det	a,expansion
R2612	T4260	T4258	pobj	expansion,to
R2613	T4261	T4260	amod	large,expansion
R2614	T4262	T4250	prep	in,led
R2615	T4263	T4262	pobj	layers,in
R2616	T4264	T4263	prep	with,layers
R2617	T4265	T4266	amod	spinous,morphology
R2618	T4266	T4264	pobj	morphology,with
R2619	T4267	T4265	cc	and,spinous
R262	T1057	T1054	conj	cord,lungs
R2620	T4268	T4265	conj	granular,spinous
R2621	T4269	T4250	punct	.,led
R2622	T4271	T4272	advmod	Additionally,was
R2623	T4273	T4272	punct	", ",was
R2624	T4274	T4272	advmod	however,was
R2625	T4275	T4272	punct	", ",was
R2626	T4276	T4277	det	a,expansion
R2627	T4277	T4272	attr	expansion,was
R2628	T4278	T4277	amod	marked,expansion
R2629	T4279	T4278	cc	and,marked
R263	T1058	T1057	punct	", ",cord
R2630	T4280	T4278	conj	variable,marked
R2631	T4281	T4277	prep	of,expansion
R2632	T4282	T4281	pobj	keratin,of
R2633	T4283	T4282	nummod	5,keratin
R2634	T4284	T4282	punct	(,keratin
R2635	T4285	T4282	appos	K5,keratin
R2636	T4286	T4282	punct	),keratin
R2637	T4287	T4282	punct	", ",keratin
R2638	T4288	T4289	advmod	normally,restricted
R2639	T4289	T4282	acl	restricted,keratin
R264	T1059	T1060	amod	mammary,glands
R2640	T4290	T4289	prep	to,restricted
R2641	T4291	T4292	det	the,layer
R2642	T4292	T4290	pobj	layer,to
R2643	T4293	T4292	amod	innermost,layer
R2644	T4294	T4292	amod	basal,layer
R2645	T4295	T4296	punct	(,see
R2646	T4296	T4272	parataxis	see,was
R2647	T4297	T4296	dobj	Figure,see
R2648	T4298	T4297	nummod	3,Figure
R2649	T4299	T4296	punct	),see
R265	T1060	T1057	conj	glands,cord
R2650	T4300	T4272	punct	.,was
R2651	T4302	T4303	mark	Although,precluded
R2652	T4303	T4318	advcl	precluded,emerged
R2653	T4304	T4305	det	the,failure
R2654	T4305	T4303	nsubj	failure,precluded
R2655	T4306	T4305	prep	of,failure
R2656	T4307	T4308	nmod	Snail,mice
R2657	T4308	T4306	pobj	mice,of
R2658	T4309	T4307	punct	-,Snail
R2659	T4310	T4307	amod	null,Snail
R266	T1061	T1060	punct	", ",glands
R2660	T4311	T4312	aux	to,develop
R2661	T4312	T4305	acl	develop,failure
R2662	T4313	T4314	amod	past,gastrulation
R2663	T4314	T4312	dobj	gastrulation,develop
R2664	T4315	T4316	punct	[,21
R2665	T4316	T4305	parataxis	21,failure
R2666	T4317	T4316	punct	],21
R2667	T4319	T4320	poss	our,ability
R2668	T4320	T4303	dobj	ability,precluded
R2669	T4321	T4322	aux	to,study
R267	T1062	T1060	cc	and,glands
R2670	T4322	T4320	acl	study,ability
R2671	T4323	T4324	det	the,loss
R2672	T4324	T4322	dobj	loss,study
R2673	T4325	T4324	prep	of,loss
R2674	T4326	T4327	compound	Snail,function
R2675	T4327	T4325	pobj	function,of
R2676	T4328	T4324	prep	in,loss
R2677	T4329	T4330	compound	skin,development
R2678	T4330	T4328	pobj	development,in
R2679	T4331	T4318	punct	", ",emerged
R268	T1063	T1064	compound	hair,follicles
R2680	T4332	T4333	det	a,correlation
R2681	T4333	T4318	nsubj	correlation,emerged
R2682	T4334	T4333	amod	good,correlation
R2683	T4335	T4318	prep	between,emerged
R2684	T4336	T4337	det	the,expression
R2685	T4337	T4335	pobj	expression,between
R2686	T4338	T4337	prep	of,expression
R2687	T4339	T4340	compound	Snail,protein
R2688	T4340	T4338	pobj	protein,of
R2689	T4341	T4337	cc	and,expression
R269	T1064	T1060	conj	follicles,glands
R2690	T4342	T4343	det	the,extension
R2691	T4343	T4337	conj	extension,expression
R2692	T4344	T4343	prep	of,extension
R2693	T4345	T4344	pobj	K5,of
R2694	T4346	T4345	punct	", ",K5
R2695	T4347	T4345	conj	Ki67,K5
R2696	T4348	T4347	punct	", ",Ki67
R2697	T4349	T4347	cc	and,Ki67
R2698	T4350	T4347	conj	pMAPK,Ki67
R2699	T4351	T4343	advmod	suprabasally,extension
R27	T177	T163	dobj	signals,exchange
R270	T1065	T1066	punct	[,1
R2700	T4352	T4353	punct	(,compare
R2701	T4353	T4318	parataxis	compare,emerged
R2702	T4354	T4353	dobj	data,compare
R2703	T4355	T4353	prep	in,compare
R2704	T4356	T4357	nmod	Figures,2
R2705	T4357	T4355	pobj	2,in
R2706	T4358	T4357	cc	and,2
R2707	T4359	T4357	conj	3,2
R2708	T4360	T4353	punct	),compare
R2709	T4361	T4318	punct	.,emerged
R271	T1066	T1046	parataxis	1,initiates
R2710	T4363	T4364	det	The,changes
R2711	T4364	T4365	nsubjpass	changes,accompanied
R2712	T4366	T4364	prep	in,changes
R2713	T4367	T4366	pobj	hyperproliferation,in
R2714	T4368	T4367	cc	and,hyperproliferation
R2715	T4369	T4367	conj	differentiation,hyperproliferation
R2716	T4370	T4365	auxpass	were,accompanied
R2717	T4371	T4365	neg	not,accompanied
R2718	T4372	T4365	advmod	initially,accompanied
R2719	T4373	T4365	agent	by,accompanied
R272	T1067	T1066	punct	],1
R2720	T4374	T4375	amod	gross,signs
R2721	T4375	T4373	pobj	signs,by
R2722	T4376	T4375	prep	of,signs
R2723	T4377	T4378	amod	epithelial,invaginations
R2724	T4378	T4376	pobj	invaginations,of
R2725	T4379	T4365	punct	.,accompanied
R2726	T4381	T4382	prep	With,developed
R2727	T4383	T4381	pobj	age,With
R2728	T4384	T4382	punct	", ",developed
R2729	T4385	T4382	advmod	however,developed
R273	T1068	T1046	punct	.,initiates
R2730	T4386	T4382	punct	", ",developed
R2731	T4387	T4388	amod	epidermal,folds
R2732	T4388	T4382	nsubj	folds,developed
R2733	T4389	T4388	cc	and,folds
R2734	T4390	T4388	conj	undulations,folds
R2735	T4391	T4382	prep	in,developed
R2736	T4392	T4391	pobj	areas,in
R2737	T4393	T4394	advmod	where,expressed
R2738	T4394	T4392	relcl	expressed,areas
R2739	T4395	T4394	nsubjpass	Snail,expressed
R274	T1070	T1071	det	The,cells
R2740	T4396	T4394	auxpass	was,expressed
R2741	T4397	T4382	punct	", ",developed
R2742	T4398	T4382	cc	and,developed
R2743	T4399	T4400	amod	proliferative,markers
R2744	T4400	T4401	nsubj	markers,persisted
R2745	T4401	T4382	conj	persisted,developed
R2746	T4402	T4401	prep	in,persisted
R2747	T4403	T4404	det	these,regions
R2748	T4404	T4402	pobj	regions,in
R2749	T4405	T4406	punct	(,staining
R275	T1071	T1076	nsubjpass	cells,attached
R2750	T4406	T4401	parataxis	staining,persisted
R2751	T4407	T4408	compound	Figure,3I
R2752	T4408	T4406	dep	3I,staining
R2753	T4409	T4408	cc	and,3I
R2754	T4410	T4408	conj	3J,3I
R2755	T4411	T4406	punct	;,staining
R2756	T4412	T4413	amod	anti-cyclin,D
R2757	T4413	T4406	compound	D,staining
R2758	T4414	T4406	punct	),staining
R2759	T4415	T4382	punct	.,developed
R276	T1072	T1071	amod	multipotent,cells
R2760	T4417	T4418	det	The,undulations
R2761	T4418	T4419	nsubjpass	undulations,accompanied
R2762	T4420	T4419	auxpass	were,accompanied
R2763	T4421	T4419	agent	by,accompanied
R2764	T4422	T4423	amod	partial,dissolution
R2765	T4423	T4421	pobj	dissolution,by
R2766	T4424	T4423	prep	of,dissolution
R2767	T4425	T4426	det	the,membrane
R2768	T4426	T4424	pobj	membrane,of
R2769	T4427	T4426	amod	underlying,membrane
R277	T1073	T1071	punct	", ",cells
R2770	T4428	T4426	compound	basement,membrane
R2771	T4429	T4430	punct	(,3K
R2772	T4430	T4419	parataxis	3K,accompanied
R2773	T4431	T4430	compound	Figure,3K
R2774	T4432	T4430	cc	and,3K
R2775	T4433	T4430	conj	3L,3K
R2776	T4434	T4430	punct	),3K
R2777	T4435	T4419	punct	.,accompanied
R2778	T4437	T4438	amod	Aberrant,staining
R2779	T4438	T4439	nsubjpass	staining,observed
R278	T1074	T1071	amod	adhering,cells
R2780	T4440	T4439	auxpass	was,observed
R2781	T4441	T4439	advmod	also,observed
R2782	T4442	T4439	prep	with,observed
R2783	T4443	T4442	pobj	antibodies,with
R2784	T4444	T4443	prep	against,antibodies
R2785	T4445	T4444	pobj	components,against
R2786	T4446	T4445	prep	of,components
R2787	T4447	T4448	det	the,hemidesmosomes
R2788	T4448	T4446	pobj	hemidesmosomes,of
R2789	T4449	T4445	punct	", ",components
R279	T1075	T1071	amod	epithelial,cells
R2790	T4450	T4451	dep	which,provide
R2791	T4451	T4445	relcl	provide,components
R2792	T4452	T4453	amod	strong,adhesion
R2793	T4453	T4451	dobj	adhesion,provide
R2794	T4454	T4453	prep	of,adhesion
R2795	T4455	T4456	amod	basal,cells
R2796	T4456	T4454	pobj	cells,of
R2797	T4457	T4456	amod	epidermal,cells
R2798	T4458	T4451	prep	to,provide
R2799	T4459	T4460	det	the,lamina
R28	T178	T163	prep	with,exchange
R280	T1077	T1076	auxpass	are,attached
R2800	T4460	T4458	pobj	lamina,to
R2801	T4461	T4460	amod	underlying,lamina
R2802	T4462	T4460	amod	basal,lamina
R2803	T4463	T4464	punct	(,3M
R2804	T4464	T4439	parataxis	3M,observed
R2805	T4465	T4464	compound	Figure,3M
R2806	T4466	T4464	cc	and,3M
R2807	T4467	T4464	conj	3N,3M
R2808	T4468	T4464	punct	),3M
R2809	T4469	T4439	punct	.,observed
R281	T1078	T1076	advmod	typically,attached
R2810	T4471	T4472	advmod	Interestingly,occur
R2811	T4473	T4472	punct	", ",occur
R2812	T4474	T4475	amod	similar,types
R2813	T4475	T4472	nsubj	types,occur
R2814	T4476	T4475	prep	of,types
R2815	T4477	T4476	pobj	alterations,of
R2816	T4478	T4472	prep	in,occur
R2817	T4479	T4480	det	the,membrane
R2818	T4480	T4478	pobj	membrane,in
R2819	T4481	T4480	compound	basement,membrane
R282	T1079	T1076	prep	to,attached
R2820	T4482	T4472	prep	in,occur
R2821	T4483	T4484	det	the,bud
R2822	T4484	T4482	pobj	bud,in
R2823	T4485	T4484	compound	hair,bud
R2824	T4486	T4484	prep	of,bud
R2825	T4487	T4488	amod	embryonic,mice
R2826	T4488	T4486	pobj	mice,of
R2827	T4489	T4487	cc	and,embryonic
R2828	T4490	T4487	conj	newborn,embryonic
R2829	T4491	T4492	advmod	when,expressed
R283	T1080	T1081	det	an,lamina
R2830	T4492	T4472	advcl	expressed,occur
R2831	T4493	T4492	nsubjpass	Snail,expressed
R2832	T4494	T4492	auxpass	is,expressed
R2833	T4495	T4492	advmod	normally,expressed
R2834	T4496	T4472	punct	.,occur
R2835	T4498	T4499	det	The,fact
R2836	T4499	T4500	nsubj	fact,suggests
R2837	T4501	T4502	mark	that,altered
R2838	T4502	T4499	acl	altered,fact
R2839	T4503	T4504	det	the,membrane
R284	T1081	T1079	pobj	lamina,to
R2840	T4504	T4502	nsubjpass	membrane,altered
R2841	T4505	T4504	compound	basement,membrane
R2842	T4506	T4504	acl	separating,membrane
R2843	T4507	T4508	det	the,epidermis
R2844	T4508	T4506	dobj	epidermis,separating
R2845	T4509	T4506	prep	from,separating
R2846	T4510	T4511	det	the,dermis
R2847	T4511	T4509	pobj	dermis,from
R2848	T4512	T4502	auxpass	is,altered
R2849	T4513	T4514	advmod	only,in
R285	T1082	T1081	amod	underlying,lamina
R2850	T4514	T4502	prep	in,altered
R2851	T4515	T4516	det	the,animals
R2852	T4516	T4514	pobj	animals,in
R2853	T4517	T4516	amod	adult,animals
R2854	T4518	T4516	compound	Tg,animals
R2855	T4519	T4520	det	the,involvement
R2856	T4520	T4500	dobj	involvement,suggests
R2857	T4521	T4520	prep	of,involvement
R2858	T4522	T4523	amod	intermediary,factors
R2859	T4523	T4521	pobj	factors,of
R286	T1083	T1081	amod	basal,lamina
R2860	T4524	T4525	neg	not,available
R2861	T4525	T4523	relcl	available,factors
R2862	T4526	T4527	advmod	as,readily
R2863	T4527	T4525	advmod	readily,available
R2864	T4528	T4525	prep	in,available
R2865	T4529	T4530	det	the,epidermis
R2866	T4530	T4528	pobj	epidermis,in
R2867	T4531	T4532	mark	as,are
R2868	T4532	T4525	advcl	are,available
R2869	T4533	T4532	nsubj	they,are
R287	T1084	T1085	dep	that,polarizes
R2870	T4534	T4532	prep	in,are
R2871	T4535	T4536	det	the,follicle
R2872	T4536	T4534	pobj	follicle,in
R2873	T4537	T4500	punct	.,suggests
R2878	T5218	T5219	amod	Possible,Links
R2879	T5220	T5219	prep	between,Links
R288	T1085	T1081	relcl	polarizes,lamina
R2880	T5221	T5222	amod	Epidermal,Hyperproliferation
R2881	T5222	T5220	pobj	Hyperproliferation,between
R2882	T5223	T5222	cc	and,Hyperproliferation
R2883	T5224	T5225	amod	Down,regulation
R2884	T5225	T5222	conj	regulation,Hyperproliferation
R2885	T5226	T5225	punct	-,regulation
R2886	T5227	T5225	prep	of,regulation
R2887	T5228	T5229	compound	AJ,Proteins
R2888	T5229	T5227	pobj	Proteins,of
R2889	T5230	T5219	prep	in,Links
R289	T1086	T1087	det	the,sheet
R2890	T5231	T5232	compound	Snail,Mice
R2891	T5232	T5230	pobj	Mice,in
R2892	T5233	T5232	compound	Tg,Mice
R2893	T5235	T5236	prep	Given,examined
R2894	T5237	T5238	mark	that,is
R2895	T5238	T5235	pcomp	is,Given
R2896	T5239	T5240	det	the,promoter
R2897	T5240	T5238	nsubj	promoter,is
R2898	T5241	T5242	compound	E,cadherin
R2899	T5242	T5240	compound	cadherin,promoter
R29	T179	T180	poss	their,neighbors
R290	T1087	T1085	dobj	sheet,polarizes
R2900	T5243	T5242	punct	-,cadherin
R2901	T5244	T5245	det	a,target
R2902	T5245	T5238	attr	target,is
R2903	T5246	T5245	amod	direct,target
R2904	T5247	T5245	prep	for,target
R2905	T5248	T5249	npadvmod	Snail,mediated
R2906	T5249	T5251	amod	mediated,repression
R2907	T5250	T5249	punct	-,mediated
R2908	T5251	T5247	pobj	repression,for
R2909	T5252	T5253	advmod	in,vitro
R291	T1088	T1087	amod	epithelial,sheet
R2910	T5253	T5238	advmod	vitro,is
R2911	T5254	T5255	punct	[,13
R2912	T5255	T5238	parataxis	13,is
R2913	T5256	T5255	nummod	4,13
R2914	T5257	T5255	punct	",",13
R2915	T5258	T5255	nummod	12,13
R2916	T5259	T5255	punct	",",13
R2917	T5260	T5255	punct	],13
R2918	T5261	T5238	punct	", ",is
R2919	T5262	T5238	cc	and,is
R292	T1089	T1085	cc	and,polarizes
R2920	T5263	T5264	mark	that,regulated
R2921	T5264	T5238	conj	regulated,is
R2922	T5265	T5266	compound	E,cadherin
R2923	T5266	T5264	nsubjpass	cadherin,regulated
R2924	T5267	T5266	punct	-,cadherin
R2925	T5268	T5264	auxpass	was,regulated
R2926	T5269	T5264	advmod	down,regulated
R2927	T5270	T5264	punct	-,regulated
R2928	T5271	T5264	prep	in,regulated
R2929	T5272	T5273	npadvmod	Snail,expressing
R293	T1090	T1085	conj	separates,polarizes
R2930	T5273	T5275	amod	expressing,buds
R2931	T5274	T5273	punct	-,expressing
R2932	T5275	T5271	pobj	buds,in
R2933	T5276	T5275	compound	hair,buds
R2934	T5277	T5236	punct	", ",examined
R2935	T5278	T5236	nsubj	we,examined
R2936	T5279	T5280	det	the,status
R2937	T5280	T5236	dobj	status,examined
R2938	T5281	T5280	prep	of,status
R2939	T5282	T5283	compound	E,cadherin
R294	T1091	T1090	dobj	it,separates
R2940	T5283	T5281	pobj	cadherin,of
R2941	T5284	T5283	punct	-,cadherin
R2942	T5285	T5283	cc	and,cadherin
R2943	T5286	T5287	amod	other,proteins
R2944	T5287	T5283	conj	proteins,cadherin
R2945	T5288	T5287	compound	AJ,proteins
R2946	T5289	T5236	prep	within,examined
R2947	T5290	T5289	pobj	regions,within
R2948	T5291	T5290	prep	of,regions
R2949	T5292	T5293	amod	hyperproliferative,epidermis
R295	T1092	T1090	prep	from,separates
R2950	T5293	T5291	pobj	epidermis,of
R2951	T5294	T5295	advmod	where,was
R2952	T5295	T5290	relcl	was,regions
R2953	T5296	T5297	compound	Tg,Snail
R2954	T5297	T5295	nsubj	Snail,was
R2955	T5298	T5295	acomp	present,was
R2956	T5299	T5300	punct	(,4A
R2957	T5300	T5236	parataxis	4A,examined
R2958	T5301	T5300	compound	Figure,4A
R2959	T5302	T5300	punct	),4A
R296	T1093	T1094	amod	surrounding,mesenchyme
R2960	T5303	T5236	punct	.,examined
R2961	T5305	T5306	prep	In,diminished
R2962	T5307	T5308	det	these,regions
R2963	T5308	T5305	pobj	regions,In
R2964	T5309	T5306	punct	", ",diminished
R2965	T5310	T5311	compound	immunofluorescence,staining
R2966	T5311	T5306	nsubjpass	staining,diminished
R2967	T5312	T5311	prep	of,staining
R2968	T5313	T5314	compound	E,cadherin
R2969	T5314	T5312	pobj	cadherin,of
R297	T1094	T1092	pobj	mesenchyme,from
R2970	T5315	T5314	punct	-,cadherin
R2971	T5316	T5314	cc	and,cadherin
R2972	T5317	T5318	compound	α,catenin
R2973	T5318	T5314	conj	catenin,cadherin
R2974	T5319	T5318	punct	-,catenin
R2975	T5320	T5306	auxpass	were,diminished
R2976	T5321	T5306	advmod	markedly,diminished
R2977	T5322	T5306	punct	.,diminished
R2978	T5324	T5325	prep	In,was
R2979	T5326	T5324	pobj	contrast,In
R298	T1095	T1076	punct	.,attached
R2980	T5327	T5325	punct	", ",was
R2981	T5328	T5329	det	the,intensity
R2982	T5329	T5325	nsubj	intensity,was
R2983	T5330	T5329	prep	of,intensity
R2984	T5331	T5332	compound	antibody,staining
R2985	T5332	T5330	pobj	staining,of
R2986	T5333	T5329	prep	for,intensity
R2987	T5334	T5335	nummod	two,proteins
R2988	T5335	T5333	pobj	proteins,for
R2989	T5336	T5335	amod	other,proteins
R299	T1097	T1098	amod	Budding,morphogenesis
R2990	T5337	T5335	compound	AJ,proteins
R2991	T5338	T5335	punct	", ",proteins
R2992	T5339	T5340	compound	β,catenin
R2993	T5340	T5335	appos	catenin,proteins
R2994	T5341	T5340	punct	-,catenin
R2995	T5342	T5340	cc	and,catenin
R2996	T5343	T5340	conj	Ajuba,catenin
R2997	T5344	T5325	punct	", ",was
R2998	T5345	T5325	advmod	still,was
R2999	T5346	T5325	acomp	strong,was
R3	T150	T147	acl	Involving,Pathway
R30	T180	T178	pobj	neighbors,with
R300	T1098	T1099	nsubjpass	morphogenesis,guided
R3000	T5347	T5325	punct	.,was
R3001	T5349	T5350	advmod	Interestingly,appeared
R3002	T5351	T5350	punct	", ",appeared
R3003	T5352	T5350	advmod	however,appeared
R3004	T5353	T5350	punct	", ",appeared
R3005	T5354	T5350	prep	despite,appeared
R3006	T5355	T5356	amod	appreciable,immunofluorescence
R3007	T5356	T5354	pobj	immunofluorescence,despite
R3008	T5357	T5350	punct	", ",appeared
R3009	T5358	T5350	nsubj	localization,appeared
R301	T1100	T1099	auxpass	is,guided
R3010	T5359	T5358	prep	of,localization
R3011	T5360	T5361	compound	β,catenin
R3012	T5361	T5359	pobj	catenin,of
R3013	T5362	T5361	punct	-,catenin
R3014	T5363	T5361	cc	and,catenin
R3015	T5364	T5361	conj	Ajuba,catenin
R3016	T5365	T5366	aux	to,be
R3017	T5366	T5350	xcomp	be,appeared
R3018	T5367	T5368	advmod	largely,cytoplasmic
R3019	T5368	T5366	acomp	cytoplasmic,be
R302	T1101	T1099	agent	by,guided
R3020	T5369	T5370	advmod	rather,than
R3021	T5370	T5368	cc	than,cytoplasmic
R3022	T5371	T5368	conj	at,cytoplasmic
R3023	T5372	T5373	compound	cell,cell
R3024	T5373	T5375	compound	cell,borders
R3025	T5374	T5373	punct	-,cell
R3026	T5375	T5371	pobj	borders,at
R3027	T5376	T5377	punct	(,insets
R3028	T5377	T5350	parataxis	insets,appeared
R3029	T5378	T5379	compound	Figure,4A
R303	T1102	T1103	det	a,exchange
R3030	T5379	T5377	compound	4A,insets
R3031	T5380	T5377	punct	),insets
R3032	T5381	T5350	punct	.,appeared
R3033	T5383	T5384	amod	Architectural,differences
R3034	T5384	T5385	nsubj	differences,made
R3035	T5386	T5384	prep	in,differences
R3036	T5387	T5388	det	the,epidermis
R3037	T5388	T5386	pobj	epidermis,in
R3038	T5389	T5390	compound	Western,blot
R3039	T5390	T5391	compound	blot,analyses
R304	T1103	T1101	pobj	exchange,by
R3040	T5391	T5392	nsubj	analyses,difficult
R3041	T5392	T5385	ccomp	difficult,made
R3042	T5393	T5392	advmod	somewhat,difficult
R3043	T5394	T5395	aux	to,gauge
R3044	T5395	T5392	advcl	gauge,difficult
R3045	T5396	T5385	punct	.,made
R3046	T5398	T5399	advmod	However,accentuated
R3047	T5400	T5399	punct	", ",accentuated
R3048	T5401	T5399	prep	in,accentuated
R3049	T5402	T5401	pobj	regions,in
R305	T1104	T1103	amod	reciprocal,exchange
R3050	T5403	T5404	amod	such,as
R3051	T5404	T5402	prep	as,regions
R3052	T5405	T5406	compound	ear,skin
R3053	T5406	T5404	pobj	skin,as
R3054	T5407	T5402	punct	", ",regions
R3055	T5408	T5409	advmod	where,expressed
R3056	T5409	T5402	relcl	expressed,regions
R3057	T5410	T5411	det	the,levels
R3058	T5411	T5409	nsubjpass	levels,expressed
R3059	T5412	T5411	amod	highest,levels
R306	T1105	T1103	prep	of,exchange
R3060	T5413	T5411	prep	of,levels
R3061	T5414	T5415	compound	Snail,protein
R3062	T5415	T5413	pobj	protein,of
R3063	T5416	T5409	auxpass	were,expressed
R3064	T5417	T5399	punct	", ",accentuated
R3065	T5418	T5419	det	the,effects
R3066	T5419	T5399	nsubjpass	effects,accentuated
R3067	T5420	T5399	auxpass	were,accentuated
R3068	T5421	T5399	punct	.,accentuated
R3069	T5423	T5424	prep	In,reduced
R307	T1106	T1105	pobj	signals,of
R3070	T5425	T5426	preconj	both,skin
R3071	T5426	T5423	pobj	skin,In
R3072	T5427	T5426	compound	back,skin
R3073	T5428	T5426	cc	and,skin
R3074	T5429	T5430	compound	ear,skin
R3075	T5430	T5426	conj	skin,skin
R3076	T5431	T5424	punct	", ",reduced
R3077	T5432	T5433	amod	overall,levels
R3078	T5433	T5424	nsubjpass	levels,reduced
R3079	T5434	T5433	prep	of,levels
R308	T1107	T1103	prep	between,exchange
R3080	T5435	T5436	compound	E,cadherin
R3081	T5436	T5434	pobj	cadherin,of
R3082	T5437	T5436	punct	-,cadherin
R3083	T5438	T5436	cc	and,cadherin
R3084	T5439	T5440	compound	α,catenin
R3085	T5440	T5436	conj	catenin,cadherin
R3086	T5441	T5440	punct	-,catenin
R3087	T5442	T5424	auxpass	were,reduced
R3088	T5443	T5424	punct	", ",reduced
R3089	T5444	T5424	prep	under,reduced
R309	T1108	T1107	pobj	epithelium,between
R3090	T5445	T5444	pobj	conditions,under
R3091	T5446	T5447	advmod	where,remained
R3092	T5447	T5445	relcl	remained,conditions
R3093	T5448	T5449	nmod	β,catenin
R3094	T5449	T5451	nmod	catenin,levels
R3095	T5450	T5449	punct	-,catenin
R3096	T5451	T5447	nsubj	levels,remained
R3097	T5452	T5449	cc	and,catenin
R3098	T5453	T5449	conj	Ajuba,catenin
R3099	T5454	T5447	acomp	unchanged,remained
R31	T181	T163	cc	and,exchange
R310	T1109	T1108	cc	and,epithelium
R3100	T5455	T5447	advcl	relative,remained
R3101	T5456	T5455	prep	to,relative
R3102	T5457	T5456	pobj	controls,to
R3103	T5458	T5459	punct	(,4B
R3104	T5459	T5424	parataxis	4B,reduced
R3105	T5460	T5459	compound	Figure,4B
R3106	T5461	T5459	punct	),4B
R3107	T5462	T5424	punct	.,reduced
R3108	T5464	T5465	advcl	Taken,were
R3109	T5466	T5464	advmod	together,Taken
R311	T1110	T1108	conj	mesenchyme,epithelium
R3110	T5467	T5465	punct	", ",were
R3111	T5468	T5469	det	these,data
R3112	T5469	T5465	nsubj	data,were
R3113	T5470	T5465	acomp	consistent,were
R3114	T5471	T5470	prep	with,consistent
R3115	T5472	T5473	poss	our,results
R3116	T5473	T5471	pobj	results,with
R3117	T5474	T5473	acl	obtained,results
R3118	T5475	T5474	prep	from,obtained
R3119	T5476	T5477	compound	immunofluorescence,microscopy
R312	T1111	T1112	aux	to,specify
R3120	T5477	T5475	pobj	microscopy,from
R3121	T5478	T5465	punct	.,were
R3122	T5480	T5481	advmod	A,priori
R3123	T5481	T5482	advmod	priori,be
R3124	T5483	T5482	punct	", ",be
R3125	T5484	T5485	det	the,decrease
R3126	T5485	T5482	nsubj	decrease,be
R3127	T5486	T5485	prep	in,decrease
R3128	T5487	T5488	compound	α,catenin
R3129	T5488	T5490	compound	catenin,levels
R313	T1112	T1103	advcl	specify,exchange
R3130	T5489	T5488	punct	-,catenin
R3131	T5490	T5486	pobj	levels,in
R3132	T5491	T5482	aux	could,be
R3133	T5492	T5482	prep	due,be
R3134	T5493	T5492	pcomp	to,due
R3135	T5494	T5495	preconj	either,repression
R3136	T5495	T5492	pobj	repression,due
R3137	T5496	T5495	amod	direct,repression
R3138	T5497	T5495	amod	transcriptional,repression
R3139	T5498	T5495	prep	by,repression
R314	T1113	T1114	det	the,identity
R3140	T5499	T5498	pobj	Snail,by
R3141	T5500	T5495	cc	or,repression
R3142	T5501	T5495	conj	perturbations,repression
R3143	T5502	T5501	prep	in,perturbations
R3144	T5503	T5504	compound	AJ,formation
R3145	T5504	T5502	pobj	formation,in
R3146	T5505	T5501	acl	caused,perturbations
R3147	T5506	T5505	agent	by,caused
R3148	T5507	T5508	det	the,decrease
R3149	T5508	T5506	pobj	decrease,by
R315	T1114	T1112	dobj	identity,specify
R3150	T5509	T5508	prep	in,decrease
R3151	T5510	T5511	compound	E,cadherin
R3152	T5511	T5513	compound	cadherin,expression
R3153	T5512	T5511	punct	-,cadherin
R3154	T5513	T5509	pobj	expression,in
R3155	T5514	T5513	compound	gene,expression
R3156	T5515	T5482	punct	.,be
R3157	T5517	T5518	aux	To,distinguish
R3158	T5518	T5519	advcl	distinguish,tested
R3159	T5520	T5518	prep	between,distinguish
R316	T1115	T1114	prep	of,identity
R3160	T5521	T5522	det	these,possibilities
R3161	T5522	T5520	pobj	possibilities,between
R3162	T5523	T5519	punct	", ",tested
R3163	T5524	T5519	nsubj	we,tested
R3164	T5525	T5526	mark	whether,restored
R3165	T5526	T5519	ccomp	restored,tested
R3166	T5527	T5528	compound	α,catenin
R3167	T5528	T5530	compound	catenin,levels
R3168	T5529	T5528	punct	-,catenin
R3169	T5530	T5526	nsubjpass	levels,restored
R317	T1116	T1117	det	the,organ
R3170	T5531	T5526	aux	could,restored
R3171	T5532	T5526	auxpass	be,restored
R3172	T5533	T5526	agent	by,restored
R3173	T5534	T5535	amod	exogenous,expression
R3174	T5535	T5533	pobj	expression,by
R3175	T5536	T5535	prep	of,expression
R3176	T5537	T5538	compound	E,cadherin
R3177	T5538	T5536	pobj	cadherin,of
R3178	T5539	T5538	punct	-,cadherin
R3179	T5540	T5526	prep	in,restored
R318	T1117	T1115	pobj	organ,of
R3180	T5541	T5542	npadvmod	Snail,expressing
R3181	T5542	T5544	amod	expressing,keratinocytes
R3182	T5543	T5542	punct	-,expressing
R3183	T5544	T5540	pobj	keratinocytes,in
R3184	T5545	T5519	punct	.,tested
R3185	T5547	T5548	mark	As,shown
R3186	T5548	T5549	advcl	shown,displayed
R3187	T5550	T5548	prep	in,shown
R3188	T5551	T5552	compound	Figure,4C
R3189	T5552	T5550	pobj	4C,in
R319	T1118	T1119	dep	that,form
R3190	T5553	T5549	punct	", ",displayed
R3191	T5554	T5555	advmod	transiently,transfected
R3192	T5555	T5556	amod	transfected,keratinocytes
R3193	T5556	T5549	nsubj	keratinocytes,displayed
R3194	T5557	T5556	acl	expressing,keratinocytes
R3195	T5558	T5559	npadvmod	HA,tagged
R3196	T5559	T5561	amod	tagged,Snail
R3197	T5560	T5559	punct	-,tagged
R3198	T5561	T5557	dobj	Snail,expressing
R3199	T5562	T5563	det	a,loss
R32	T182	T163	conj	develop,exchange
R320	T1119	T1117	relcl	form,organ
R3200	T5563	T5549	dobj	loss,displayed
R3201	T5564	T5563	prep	of,loss
R3202	T5565	T5566	compound	E,cadherin
R3203	T5566	T5564	pobj	cadherin,of
R3204	T5567	T5566	punct	-,cadherin
R3205	T5568	T5566	cc	and,cadherin
R3206	T5569	T5570	compound	α,catenin
R3207	T5570	T5566	conj	catenin,cadherin
R3208	T5571	T5570	punct	-,catenin
R3209	T5572	T5549	prep	at,displayed
R321	T1120	T1119	aux	will,form
R3210	T5573	T5574	compound	cell,cell
R3211	T5574	T5576	compound	cell,borders
R3212	T5575	T5574	punct	-,cell
R3213	T5576	T5572	pobj	borders,at
R3214	T5577	T5549	punct	.,displayed
R3215	T5579	T5580	nsubj	Coexpression,enabled
R3216	T5581	T5579	prep	of,Coexpression
R3217	T5582	T5583	amod	exogenous,cadherin
R3218	T5583	T5581	pobj	cadherin,of
R3219	T5584	T5585	npadvmod	HA,tagged
R322	T1121	T1112	cc	and,specify
R3220	T5585	T5583	amod	tagged,cadherin
R3221	T5586	T5585	punct	-,tagged
R3222	T5587	T5583	compound	E,cadherin
R3223	T5588	T5583	punct	-,cadherin
R3224	T5589	T5580	preconj	not,enabled
R3225	T5590	T5589	advmod	only,not
R3226	T5591	T5592	compound	cell,cell
R3227	T5592	T5594	compound	cell,localization
R3228	T5593	T5592	punct	-,cell
R3229	T5594	T5580	dobj	localization,enabled
R323	T1122	T1123	aux	to,govern
R3230	T5595	T5594	compound	border,localization
R3231	T5596	T5594	prep	of,localization
R3232	T5597	T5598	compound	E,cadherin
R3233	T5598	T5600	compound	cadherin,protein
R3234	T5599	T5598	punct	-,cadherin
R3235	T5600	T5596	pobj	protein,of
R3236	T5601	T5580	punct	", ",enabled
R3237	T5602	T5580	cc	but,enabled
R3238	T5603	T5602	advmod	also,but
R3239	T5604	T5580	conj	rescued,enabled
R324	T1123	T1112	conj	govern,specify
R3240	T5605	T5606	det	the,staining
R3241	T5606	T5604	dobj	staining,rescued
R3242	T5607	T5608	compound	cell,cell
R3243	T5608	T5606	compound	cell,staining
R3244	T5609	T5608	punct	-,cell
R3245	T5610	T5606	compound	border,staining
R3246	T5611	T5606	prep	of,staining
R3247	T5612	T5613	compound	α,catenin
R3248	T5613	T5611	pobj	catenin,of
R3249	T5614	T5613	punct	-,catenin
R325	T1124	T1125	poss	its,growth
R3250	T5615	T5616	punct	(,4C
R3251	T5616	T5604	parataxis	4C,rescued
R3252	T5617	T5616	compound	Figure,4C
R3253	T5618	T5616	punct	),4C
R3254	T5619	T5580	punct	.,enabled
R3255	T5621	T5622	det	The,ability
R3256	T5622	T5623	nsubj	ability,argues
R3257	T5624	T5625	aux	to,restore
R3258	T5625	T5622	acl	restore,ability
R3259	T5626	T5627	compound	α,catenin
R326	T1125	T1123	dobj	growth,govern
R3260	T5627	T5625	dobj	catenin,restore
R3261	T5628	T5627	punct	-,catenin
R3262	T5629	T5627	appos	expression,catenin
R3263	T5630	T5629	cc	and,expression
R3264	T5631	T5629	conj	localization,expression
R3265	T5632	T5625	prep	under,restore
R3266	T5633	T5634	det	these,conditions
R3267	T5634	T5632	pobj	conditions,under
R3268	T5635	T5623	prep	against,argues
R3269	T5636	T5637	det	the,notion
R327	T1126	T1099	punct	.,guided
R3270	T5637	T5635	pobj	notion,against
R3271	T5638	T5639	mark	that,represses
R3272	T5639	T5637	acl	represses,notion
R3273	T5640	T5639	nsubj	Snail,represses
R3274	T5641	T5639	advmod	transcriptionally,represses
R3275	T5642	T5643	compound	α,catenin
R3276	T5643	T5639	dobj	catenin,represses
R3277	T5644	T5643	punct	-,catenin
R3278	T5645	T5623	punct	.,argues
R3279	T5647	T5648	advmod	Rather,are
R328	T1128	T1129	prep	At,are
R3280	T5649	T5648	punct	", ",are
R3281	T5650	T5651	det	the,findings
R3282	T5651	T5648	nsubj	findings,are
R3283	T5652	T5648	acomp	consistent,are
R3284	T5653	T5652	prep	with,consistent
R3285	T5654	T5655	det	a,report
R3286	T5655	T5653	pobj	report,with
R3287	T5656	T5655	amod	previous,report
R3288	T5657	T5658	mark	that,required
R3289	T5658	T5655	acl	required,report
R329	T1130	T1131	det	the,helm
R3290	T5659	T5660	compound	E,cadherin
R3291	T5660	T5658	nsubjpass	cadherin,required
R3292	T5661	T5660	punct	-,cadherin
R3293	T5662	T5658	auxpass	is,required
R3294	T5663	T5658	prep	for,required
R3295	T5664	T5665	det	the,translation
R3296	T5665	T5663	pobj	translation,for
R3297	T5666	T5665	prep	of,translation
R3298	T5667	T5668	compound	α,catenin
R3299	T5668	T5670	compound	catenin,mRNA
R33	T183	T182	prep	into,develop
R330	T1131	T1128	pobj	helm,At
R3300	T5669	T5668	punct	-,catenin
R3301	T5670	T5666	pobj	mRNA,of
R3302	T5671	T5672	punct	[,22
R3303	T5672	T5648	parataxis	22,are
R3304	T5673	T5672	punct	],22
R3305	T5674	T5648	punct	.,are
R3306	T5676	T5677	prep	Despite,displayed
R3307	T5678	T5679	det	the,reductions
R3308	T5679	T5676	pobj	reductions,Despite
R3309	T5680	T5679	prep	in,reductions
R331	T1132	T1131	prep	of,helm
R3310	T5681	T5682	compound	AJ,markers
R3311	T5682	T5680	pobj	markers,in
R3312	T5683	T5677	punct	", ",displayed
R3313	T5684	T5685	compound	Tg,skin
R3314	T5685	T5677	nsubj	skin,displayed
R3315	T5686	T5677	advmod	still,displayed
R3316	T5687	T5688	amod	sealed,membranes
R3317	T5688	T5677	dobj	membranes,displayed
R3318	T5689	T5688	cc	and,membranes
R3319	T5690	T5691	amod	intercellular,junctions
R332	T1133	T1134	det	these,pathways
R3320	T5691	T5688	conj	junctions,membranes
R3321	T5692	T5693	dep	that,were
R3322	T5693	T5691	relcl	were,junctions
R3323	T5694	T5693	advmod	largely,were
R3324	T5695	T5693	acomp	intact,were
R3325	T5696	T5693	punct	", ",were
R3326	T5697	T5698	mark	as,judged
R3327	T5698	T5693	advcl	judged,were
R3328	T5699	T5698	prep	by,judged
R3329	T5700	T5701	amod	ultrastructural,analyses
R333	T1134	T1132	pobj	pathways,of
R3330	T5701	T5699	pobj	analyses,by
R3331	T5702	T5703	punct	(,data
R3332	T5703	T5677	meta	data,displayed
R3333	T5704	T5703	amod	unpublished,data
R3334	T5705	T5703	punct	),data
R3335	T5706	T5677	punct	.,displayed
R3336	T5708	T5709	prep	In,resembled
R3337	T5710	T5711	det	this,respect
R3338	T5711	T5708	pobj	respect,In
R3339	T5712	T5709	punct	", ",resembled
R334	T1135	T1136	amod	molecular,communication
R3340	T5713	T5714	det	the,epithelium
R3341	T5714	T5709	nsubj	epithelium,resembled
R3342	T5715	T5714	compound	skin,epithelium
R3343	T5716	T5709	dobj	that,resembled
R3344	T5717	T5716	prep	of,that
R3345	T5718	T5719	det	the,bud
R3346	T5719	T5717	pobj	bud,of
R3347	T5720	T5719	compound	hair,bud
R3348	T5721	T5719	punct	", ",bud
R3349	T5722	T5723	advmod	where,is
R335	T1136	T1134	compound	communication,pathways
R3350	T5723	T5719	relcl	is,bud
R3351	T5724	T5725	det	the,regulation
R3352	T5725	T5723	nsubj	regulation,is
R3353	T5726	T5725	amod	down,regulation
R3354	T5727	T5725	punct	-,regulation
R3355	T5728	T5725	prep	in,regulation
R3356	T5729	T5730	compound	junction,proteins
R3357	T5730	T5728	pobj	proteins,in
R3358	T5731	T5723	acomp	permissive,is
R3359	T5732	T5723	prep	for,is
R336	T1137	T1129	nsubj	Wnts,are
R3360	T5733	T5734	compound	cell,cell
R3361	T5734	T5736	compound	cell,remodeling
R3362	T5735	T5734	punct	-,cell
R3363	T5736	T5732	pobj	remodeling,for
R3364	T5737	T5723	prep	without,is
R3365	T5738	T5737	pcomp	abrogating,without
R3366	T5739	T5740	amod	intercellular,adhesion
R3367	T5740	T5738	dobj	adhesion,abrogating
R3368	T5741	T5709	punct	.,resembled
R3369	T5743	T5744	det	The,similarities
R337	T1138	T1137	punct	", ",Wnts
R3370	T5744	T5745	nsubj	similarities,extended
R3371	T5746	T5744	prep	between,similarities
R3372	T5747	T5748	compound	Snail,epidermis
R3373	T5748	T5746	pobj	epidermis,between
R3374	T5749	T5748	compound	Tg,epidermis
R3375	T5750	T5748	cc	and,epidermis
R3376	T5751	T5752	compound	hair,buds
R3377	T5752	T5748	conj	buds,epidermis
R3378	T5753	T5745	prep	to,extended
R3379	T5754	T5755	det	the,state
R338	T1139	T1140	nmod	bone,proteins
R3380	T5755	T5753	pobj	state,to
R3381	T5756	T5755	amod	hyperproliferative,state
R3382	T5757	T5745	punct	", ",extended
R3383	T5758	T5745	advcl	leading,extended
R3384	T5759	T5758	dobj	us,leading
R3385	T5760	T5761	aux	to,wonder
R3386	T5761	T5758	xcomp	wonder,leading
R3387	T5762	T5763	mark	whether,contribute
R3388	T5763	T5761	ccomp	contribute,wonder
R3389	T5764	T5765	det	the,regulation
R339	T1140	T1137	appos	proteins,Wnts
R3390	T5765	T5763	nsubj	regulation,contribute
R3391	T5766	T5765	amod	down,regulation
R3392	T5767	T5765	punct	-,regulation
R3393	T5768	T5765	prep	of,regulation
R3394	T5769	T5770	compound	AJ,proteins
R3395	T5770	T5768	pobj	proteins,of
R3396	T5771	T5763	aux	might,contribute
R3397	T5772	T5763	prep	to,contribute
R3398	T5773	T5774	det	this,condition
R3399	T5774	T5772	pobj	condition,to
R34	T184	T185	det	a,structure
R340	T1141	T1140	amod	morphogenic,proteins
R3400	T5775	T5745	punct	.,extended
R3401	T5777	T5778	prep	Given,used
R3402	T5779	T5780	det	the,increase
R3403	T5780	T5777	pobj	increase,Given
R3404	T5781	T5780	prep	in,increase
R3405	T5782	T5783	compound	pMAPK,staining
R3406	T5783	T5781	pobj	staining,in
R3407	T5784	T5780	prep	in,increase
R3408	T5785	T5786	compound	Snail,epidermis
R3409	T5786	T5784	pobj	epidermis,in
R341	T1142	T1140	punct	(,proteins
R3410	T5787	T5786	compound	Tg,epidermis
R3411	T5788	T5789	punct	(,see
R3412	T5789	T5780	parataxis	see,increase
R3413	T5790	T5791	compound	Figure,2G
R3414	T5791	T5789	dobj	2G,see
R3415	T5792	T5789	punct	),see
R3416	T5793	T5778	punct	", ",used
R3417	T5794	T5778	nsubj	we,used
R3418	T5795	T5796	compound	pMAPK,levels
R3419	T5796	T5778	dobj	levels,used
R342	T1143	T1140	appos	BMPs,proteins
R3420	T5797	T5778	prep	as,used
R3421	T5798	T5799	poss	our,assay
R3422	T5799	T5797	pobj	assay,as
R3423	T5800	T5801	aux	to,test
R3424	T5801	T5778	advcl	test,used
R3425	T5802	T5803	mark	whether,contributed
R3426	T5803	T5801	ccomp	contributed,test
R3427	T5804	T5805	det	the,loss
R3428	T5805	T5803	nsubj	loss,contributed
R3429	T5806	T5805	prep	of,loss
R343	T1144	T1137	punct	),Wnts
R3430	T5807	T5808	compound	E,cadherin
R3431	T5808	T5806	pobj	cadherin,of
R3432	T5809	T5808	punct	-,cadherin
R3433	T5810	T5803	prep	to,contributed
R3434	T5811	T5812	det	the,increase
R3435	T5812	T5810	pobj	increase,to
R3436	T5813	T5814	npadvmod	Snail,mediated
R3437	T5814	T5812	amod	mediated,increase
R3438	T5815	T5814	punct	-,mediated
R3439	T5816	T5812	prep	in,increase
R344	T1145	T1137	punct	", ",Wnts
R3440	T5817	T5816	pobj	proliferation,in
R3441	T5818	T5778	punct	.,used
R3442	T5820	T5821	advcl	Consistent,exhibited
R3443	T5822	T5820	prep	with,Consistent
R3444	T5823	T5824	poss	our,observations
R3445	T5824	T5822	pobj	observations,with
R3446	T5825	T5826	advmod	in,vivo
R3447	T5826	T5824	amod	vivo,observations
R3448	T5827	T5821	punct	", ",exhibited
R3449	T5828	T5829	amod	transfected,keratinocytes
R345	T1146	T1147	amod	transforming,βs
R3450	T5829	T5821	nsubj	keratinocytes,exhibited
R3451	T5830	T5829	acl	expressing,keratinocytes
R3452	T5831	T5830	dobj	Snail,expressing
R3453	T5832	T5833	det	a,increase
R3454	T5833	T5821	dobj	increase,exhibited
R3455	T5834	T5833	amod	substantial,increase
R3456	T5835	T5833	prep	in,increase
R3457	T5836	T5837	compound	pMAPK,levels
R3458	T5837	T5835	pobj	levels,in
R3459	T5838	T5833	amod	relative,increase
R346	T1147	T1137	appos	βs,Wnts
R3460	T5839	T5838	prep	to,relative
R3461	T5840	T5841	compound	control,cells
R3462	T5841	T5839	pobj	cells,to
R3463	T5842	T5843	punct	(,4D
R3464	T5843	T5821	parataxis	4D,exhibited
R3465	T5844	T5843	compound	Figure,4D
R3466	T5845	T5843	punct	),4D
R3467	T5846	T5821	punct	.,exhibited
R3468	T5848	T5849	nsubj	Coexpression,appeared
R3469	T5850	T5848	prep	of,Coexpression
R347	T1148	T1147	compound	growth,βs
R3470	T5851	T5852	compound	E,cadherin
R3471	T5852	T5850	pobj	cadherin,of
R3472	T5853	T5852	punct	-,cadherin
R3473	T5854	T5848	prep	with,Coexpression
R3474	T5855	T5854	pobj	Snail,with
R3475	T5856	T5857	aux	to,abrogate
R3476	T5857	T5849	xcomp	abrogate,appeared
R3477	T5858	T5859	det	this,effect
R3478	T5859	T5857	dobj	effect,abrogate
R3479	T5860	T5849	punct	.,appeared
R348	T1149	T1147	compound	factor,βs
R3480	T5862	T5863	advmod	Together,raised
R3481	T5864	T5863	punct	", ",raised
R3482	T5865	T5866	det	these,findings
R3483	T5866	T5863	nsubj	findings,raised
R3484	T5867	T5868	det	the,possibility
R3485	T5868	T5863	dobj	possibility,raised
R3486	T5869	T5870	mark	that,participate
R3487	T5870	T5868	acl	participate,possibility
R3488	T5871	T5872	det	an,protein
R3489	T5872	T5870	nsubj	protein,participate
R349	T1150	T1147	punct	(,βs
R3490	T5873	T5874	npadvmod	AJ,associated
R3491	T5874	T5872	amod	associated,protein
R3492	T5875	T5874	punct	-,associated
R3493	T5876	T5877	dep	that,sequestered
R3494	T5877	T5872	relcl	sequestered,protein
R3495	T5878	T5877	auxpass	is,sequestered
R3496	T5879	T5877	advmod	normally,sequestered
R3497	T5880	T5877	prep	at,sequestered
R3498	T5881	T5882	det	the,membrane
R3499	T5882	T5880	pobj	membrane,at
R35	T185	T183	pobj	structure,into
R350	T1151	T1152	compound	TGF,βs
R3500	T5883	T5882	compound	plasma,membrane
R3501	T5884	T5870	aux	may,participate
R3502	T5885	T5870	prep	in,participate
R3503	T5886	T5887	det	a,pathway
R3504	T5887	T5885	pobj	pathway,in
R3505	T5888	T5887	compound	proliferation,pathway
R3506	T5889	T5887	compound	signaling,pathway
R3507	T5890	T5891	advmod	when,deconstructed
R3508	T5891	T5870	advcl	deconstructed,participate
R3509	T5892	T5891	nsubjpass	AJs,deconstructed
R351	T1152	T1147	appos	βs,βs
R3510	T5893	T5891	auxpass	are,deconstructed
R3511	T5894	T5863	punct	.,raised
R3512	T5896	T5897	amod	Numerous,studies
R3513	T5897	T5898	nsubj	studies,correlated
R3514	T5899	T5898	aux	have,correlated
R3515	T5900	T5901	det	a,regulation
R3516	T5901	T5898	dobj	regulation,correlated
R3517	T5902	T5901	amod	down,regulation
R3518	T5903	T5901	punct	-,regulation
R3519	T5904	T5901	prep	of,regulation
R352	T1153	T1152	punct	-,βs
R3520	T5905	T5906	compound	E,cadherin
R3521	T5906	T5904	pobj	cadherin,of
R3522	T5907	T5906	punct	-,cadherin
R3523	T5908	T5898	prep	with,correlated
R3524	T5909	T5910	det	a,translocation
R3525	T5910	T5908	pobj	translocation,with
R3526	T5911	T5910	prep	of,translocation
R3527	T5912	T5913	compound	β,catenin
R3528	T5913	T5911	pobj	catenin,of
R3529	T5914	T5913	punct	-,catenin
R353	T1154	T1137	punct	),Wnts
R3530	T5915	T5910	prep	to,translocation
R3531	T5916	T5917	det	the,nucleus
R3532	T5917	T5915	pobj	nucleus,to
R3533	T5918	T5910	cc	and,translocation
R3534	T5919	T5920	det	a,transactivation
R3535	T5920	T5910	conj	transactivation,translocation
R3536	T5921	T5920	prep	of,transactivation
R3537	T5922	T5921	pobj	genes,of
R3538	T5923	T5924	dep	that,regulated
R3539	T5924	T5922	relcl	regulated,genes
R354	T1155	T1137	punct	", ",Wnts
R3540	T5925	T5924	auxpass	are,regulated
R3541	T5926	T5924	agent	by,regulated
R3542	T5927	T5928	det	the,family
R3543	T5928	T5926	pobj	family,by
R3544	T5929	T5928	nmod	LEF,family
R3545	T5930	T5929	punct	-,LEF
R3546	T5931	T5929	nummod	1,LEF
R3547	T5932	T5929	punct	/,LEF
R3548	T5933	T5934	compound	T,cell
R3549	T5934	T5935	compound	cell,factor
R355	T1156	T1137	cc	and,Wnts
R3550	T5935	T5929	appos	factor,LEF
R3551	T5936	T5935	punct	(,factor
R3552	T5937	T5935	appos	TCF,factor
R3553	T5938	T5928	punct	),family
R3554	T5939	T5928	prep	of,family
R3555	T5940	T5941	npadvmod	DNA,binding
R3556	T5941	T5942	amod	binding,proteins
R3557	T5942	T5939	pobj	proteins,of
R3558	T5943	T5944	punct	[,25
R3559	T5944	T5898	parataxis	25,correlated
R356	T1157	T1158	compound	fibroblast,factors
R3560	T5945	T5944	nummod	23,25
R3561	T5946	T5944	punct	",",25
R3562	T5947	T5944	nummod	24,25
R3563	T5948	T5944	punct	",",25
R3564	T5949	T5944	punct	],25
R3565	T5950	T5898	punct	.,correlated
R3566	T5952	T5953	det	The,presence
R3567	T5953	T5954	nsubj	presence,was
R3568	T5955	T5953	prep	of,presence
R3569	T5956	T5957	amod	nuclear,D
R357	T1158	T1137	conj	factors,Wnts
R3570	T5957	T5955	pobj	D,of
R3571	T5958	T5957	compound	cyclin,D
R3572	T5959	T5953	prep	in,presence
R3573	T5960	T5961	amod	hyperproliferative,epidermis
R3574	T5961	T5959	pobj	epidermis,in
R3575	T5962	T5961	compound	Snail,epidermis
R3576	T5963	T5961	compound	Tg,epidermis
R3577	T5964	T5965	advmod	particularly,intriguing
R3578	T5965	T5954	acomp	intriguing,was
R3579	T5966	T5967	mark	since,reported
R358	T1159	T1158	compound	growth,factors
R3580	T5967	T5954	advcl	reported,was
R3581	T5968	T5969	amod	prior,studies
R3582	T5969	T5967	nsubj	studies,reported
R3583	T5970	T5967	aux	have,reported
R3584	T5971	T5972	compound	cyclin,D
R3585	T5972	T5973	compound	D,gene
R3586	T5973	T5967	dobj	gene,reported
R3587	T5974	T5967	prep	as,reported
R3588	T5975	T5976	det	a,target
R3589	T5976	T5974	pobj	target,as
R359	T1160	T1158	punct	(,factors
R3590	T5977	T5976	amod	direct,target
R3591	T5978	T5976	prep	of,target
R3592	T5979	T5980	nmod	TCF,β
R3593	T5980	T5982	nmod	β,transcription
R3594	T5981	T5980	punct	/,β
R3595	T5982	T5978	pobj	transcription,of
R3596	T5983	T5980	punct	-,β
R3597	T5984	T5980	appos	catenin,β
R3598	T5985	T5986	punct	[,26
R3599	T5986	T5954	parataxis	26,was
R36	T186	T185	compound	bud,structure
R360	T1161	T1158	appos	FGFs,factors
R3600	T5987	T5986	punct	],26
R3601	T5988	T5954	punct	.,was
R3602	T5990	T5991	nsubj	This,said
R3603	T5991	T5992	advcl	said,detect
R3604	T5993	T5992	punct	", ",detect
R3605	T5994	T5992	nsubj	we,detect
R3606	T5995	T5992	aux	did,detect
R3607	T5996	T5992	neg	not,detect
R3608	T5997	T5998	amod	nuclear,catenin
R3609	T5998	T5992	dobj	catenin,detect
R361	T1162	T1129	punct	),are
R3610	T5999	T5998	compound	β,catenin
R3611	T6000	T5998	punct	-,catenin
R3612	T6001	T5992	prep	in,detect
R3613	T6002	T6003	poss	our,epidermis
R3614	T6003	T6001	pobj	epidermis,in
R3615	T6004	T6003	compound	Tg,epidermis
R3616	T6005	T5992	punct	", ",detect
R3617	T6006	T5992	cc	and,detect
R3618	T6007	T6008	csubj	mating,gave
R3619	T6008	T5992	conj	gave,detect
R362	T1163	T1129	punct	.,are
R3620	T6009	T6010	det	the,mice
R3621	T6010	T6007	dobj	mice,mating
R3622	T6011	T6010	compound	Snail,mice
R3623	T6012	T6010	compound	Tg,mice
R3624	T6013	T6007	prep	against,mating
R3625	T6014	T6015	det	the,mouse
R3626	T6015	T6013	pobj	mouse,against
R3627	T6016	T6015	compound	TOPGal,mouse
R3628	T6017	T6015	compound	reporter,mouse
R3629	T6018	T6019	punct	[,20
R363	T1165	T1166	prep	Through,trigger
R3630	T6019	T6007	parataxis	20,mating
R3631	T6020	T6019	punct	],20
R3632	T6021	T6022	det	no,signs
R3633	T6022	T6008	dobj	signs,gave
R3634	T6023	T6022	prep	of,signs
R3635	T6024	T6025	amod	ectopic,activity
R3636	T6025	T6023	pobj	activity,of
R3637	T6026	T6025	nmod	LEF,activity
R3638	T6027	T6026	punct	-,LEF
R3639	T6028	T6026	nummod	1,LEF
R364	T1167	T1165	pobj	activation,Through
R3640	T6029	T6026	punct	/,LEF
R3641	T6030	T6026	appos	Tcf,LEF
R3642	T6031	T6026	punct	/,LEF
R3643	T6032	T6033	compound	β,catenin
R3644	T6033	T6026	appos	catenin,LEF
R3645	T6034	T6033	punct	-,catenin
R3646	T6035	T6036	punct	(,data
R3647	T6036	T6008	meta	data,gave
R3648	T6037	T6036	amod	unpublished,data
R3649	T6038	T6036	punct	),data
R365	T1168	T1167	prep	of,activation
R3650	T6039	T5992	punct	.,detect
R3651	T6041	T6042	nsubj	We,turned
R3652	T6043	T6042	advmod	next,turned
R3653	T6044	T6042	prep	to,turned
R3654	T6045	T6046	det	the,presence
R3655	T6093	T6091	nummod	2,protein
R3656	T6046	T6044	pobj	presence,to
R3657	T6094	T6091	punct	(,protein
R3658	T6095	T6091	appos	Grb,protein
R3659	T6047	T6046	prep	of,presence
R366	T1169	T1170	compound	cell,surface
R3660	T6096	T6095	punct	-,Grb
R3661	T6097	T6095	nummod	2,Grb
R3662	T6048	T6049	amod	cytoplasmic,Ajuba
R3663	T6098	T6091	punct	),protein
R3664	T6099	T6091	punct	/,protein
R3665	T6100	T6091	appos	son,protein
R3666	T6049	T6047	pobj	Ajuba,of
R3667	T6101	T6100	prep	of,son
R3668	T6102	T6101	pobj	sevenless,of
R3669	T6103	T6100	punct	(,son
R367	T1170	T1171	compound	surface,receptors
R3670	T6050	T6042	prep	for,turned
R3671	T6104	T6100	appos	Sos,son
R3672	T6105	T6091	punct	),protein
R3673	T6106	T6091	punct	", ",protein
R3674	T6051	T6052	det	a,link
R3675	T6107	T6108	det	the,factor
R3676	T6108	T6091	appos	factor,protein
R3677	T6109	T6108	compound	nucleotide,factor
R3678	T6052	T6050	pobj	link,for
R3679	T6110	T6108	compound	exchange,factor
R368	T1171	T1168	pobj	receptors,of
R3680	T6111	T6108	prep	for,factor
R3681	T6112	T6111	pobj	Ras,for
R3682	T6053	T6052	amod	possible,link
R3683	T6113	T6112	punct	", ",Ras
R3684	T6114	T6115	dep	which,is
R3685	T6115	T6112	relcl	is,Ras
R3686	T6054	T6052	amod	mechanistic,link
R3687	T6116	T6115	advmod	upstream,is
R3688	T6117	T6116	prep	from,upstream
R3689	T6118	T6117	pobj	activation,from
R369	T1172	T1171	compound	transmembrane,receptors
R3690	T6119	T6118	prep	of,activation
R3691	T6055	T6052	prep	to,link
R3692	T6120	T6119	pobj	MAPK,of
R3693	T6121	T6122	punct	[,9
R3694	T6056	T6057	det	the,increase
R3695	T6122	T6067	parataxis	9,associate
R3696	T6123	T6122	punct	],9
R3697	T6124	T6067	punct	.,associate
R3698	T6057	T6055	pobj	increase,to
R3699	T6126	T6127	prep	Given,examined
R37	T187	T163	punct	.,exchange
R370	T1173	T1166	punct	", ",trigger
R3700	T6058	T6057	amod	proliferative,increase
R3701	T6128	T6129	det	the,increase
R3702	T6129	T6126	pobj	increase,Given
R3703	T6130	T6129	prep	in,increase
R3704	T6131	T6132	compound	pMAPK,staining
R3705	T6059	T6057	prep	in,increase
R3706	T6132	T6130	pobj	staining,in
R3707	T6060	T6061	poss	our,epidermis
R3708	T6133	T6129	prep	in,increase
R3709	T6134	T6135	compound	Tg,skin
R371	T1174	T1175	det	these,molecules
R3710	T6061	T6059	pobj	epidermis,in
R3711	T6135	T6133	pobj	skin,in
R3712	T6136	T6127	punct	", ",examined
R3713	T6137	T6127	nsubj	we,examined
R3714	T6062	T6061	compound	Snail,epidermis
R3715	T6138	T6139	det	the,possibility
R3716	T6139	T6127	dobj	possibility,examined
R3717	T6140	T6141	mark	that,changed
R3718	T6063	T6061	compound	Tg,epidermis
R3719	T6141	T6139	acl	changed,possibility
R372	T1175	T1166	nsubj	molecules,trigger
R3720	T6142	T6141	nsubj	Ajuba,changed
R3721	T6143	T6141	aux	might,changed
R3722	T6064	T6042	punct	.,turned
R3723	T6144	T6141	aux	have,changed
R3724	T6145	T6146	poss	its,partner
R3725	T6146	T6141	dobj	partner,changed
R3726	T6066	T6067	prep	In,associate
R3727	T6147	T6146	compound	binding,partner
R3728	T6148	T6141	prep	in,changed
R3729	T6149	T6150	npadvmod	Snail,expressing
R373	T1176	T1175	amod	external,molecules
R3730	T6150	T6152	amod	expressing,epidermis
R3731	T6151	T6150	punct	-,expressing
R3732	T6068	T6066	pobj	addition,In
R3733	T6152	T6148	pobj	epidermis,in
R3734	T6153	T6127	punct	.,examined
R3735	T6155	T6156	advmod	Interestingly,detected
R3736	T6069	T6068	prep	to,addition
R3737	T6157	T6156	punct	", ",detected
R3738	T6070	T6071	poss	its,ability
R3739	T6158	T6156	nsubjpass	Ajuba,detected
R374	T1177	T1175	compound	signaling,molecules
R3740	T6159	T6156	auxpass	was,detected
R3741	T6160	T6156	advmod	readily,detected
R3742	T6071	T6069	pobj	ability,to
R3743	T6161	T6156	prep	in,detected
R3744	T6162	T6163	amod	anti-Grb,immunoprecipitates
R3745	T6163	T6161	pobj	immunoprecipitates,in
R3746	T6072	T6071	amod	documented,ability
R3747	T6164	T6162	punct	-,anti-Grb
R3748	T6165	T6162	advmod	2,anti-Grb
R3749	T6073	T6074	aux	to,bind
R375	T1178	T1179	amod	distinct,cascades
R3750	T6166	T6163	prep	of,immunoprecipitates
R3751	T6167	T6168	compound	protein,lysates
R3752	T6168	T6166	pobj	lysates,of
R3753	T6074	T6071	acl	bind,ability
R3754	T6169	T6168	prep	from,lysates
R3755	T6170	T6169	pobj	skins,from
R3756	T6171	T6170	prep	of,skins
R3757	T6075	T6076	compound	α,catenin
R3758	T6172	T6173	compound	Snail,mice
R3759	T6173	T6171	pobj	mice,of
R376	T1179	T1166	dobj	cascades,trigger
R3760	T6076	T6074	dobj	catenin,bind
R3761	T6174	T6173	compound	Tg,mice
R3762	T6175	T6169	cc	but,from
R3763	T6176	T6175	neg	not,but
R3764	T6077	T6076	punct	-,catenin
R3765	T6177	T6169	conj	from,from
R3766	T6178	T6179	det	the,animals
R3767	T6078	T6079	punct	[,10
R3768	T6179	T6177	pobj	animals,from
R3769	T6180	T6179	amod	corresponding,animals
R377	T1180	T1179	prep	of,cascades
R3770	T6181	T6182	amod	wild,type
R3771	T6079	T6068	parataxis	10,addition
R3772	T6182	T6179	nmod	type,animals
R3773	T6183	T6182	punct	-,type
R3774	T6184	T6182	punct	(,type
R3775	T6080	T6079	punct	],10
R3776	T6185	T6182	appos	WT,type
R3777	T6186	T6179	punct	),animals
R3778	T6081	T6067	punct	", ",associate
R3779	T6187	T6188	punct	(,4E
R378	T1181	T1182	amod	intracellular,events
R3780	T6188	T6156	parataxis	4E,detected
R3781	T6189	T6188	compound	Figure,4E
R3782	T6082	T6067	nsubj	Ajuba,associate
R3783	T6190	T6188	punct	),4E
R3784	T6191	T6156	punct	.,detected
R3785	T6083	T6067	aux	can,associate
R3786	T6193	T6194	advmod	When,repeated
R3787	T6194	T6198	advcl	repeated,detected
R3788	T6195	T6196	det	these,experiments
R3789	T6084	T6067	advmod	also,associate
R379	T1182	T1180	pobj	events,of
R3790	T6196	T6194	nsubjpass	experiments,repeated
R3791	T6197	T6194	auxpass	were,repeated
R3792	T6085	T6067	prep	with,associate
R3793	T6086	T6087	compound	growth,factor
R3794	T6087	T6088	compound	factor,receptor
R3795	T6199	T6194	prep	with,repeated
R3796	T6088	T6089	npadvmod	receptor,bound
R3797	T6200	T6201	compound	α,catenin
R3798	T6201	T6203	npadvmod	catenin,null
R3799	T6202	T6201	punct	-,catenin
R38	T189	T190	advcl	Using,employed
R380	T1183	T1184	dep	that,culminate
R3800	T6089	T6091	amod	bound,protein
R3801	T6203	T6205	amod	null,epidermis
R3802	T6204	T6203	punct	-,null
R3803	T6205	T6199	pobj	epidermis,with
R3804	T6090	T6089	punct	-,bound
R3805	T6206	T6198	punct	", ",detected
R3806	T6207	T6208	det	a,association
R3807	T6208	T6198	nsubjpass	association,detected
R3808	T6209	T6208	amod	similar,association
R3809	T6210	T6211	nmod	Grb,Ajuba
R381	T1184	T1179	relcl	culminate,cascades
R3810	T6211	T6208	compound	Ajuba,association
R3811	T6091	T6085	pobj	protein,with
R3812	T6212	T6211	punct	-,Ajuba
R3813	T6213	T6211	nummod	2,Ajuba
R3814	T6214	T6211	punct	-,Ajuba
R3815	T6215	T6198	auxpass	was,detected
R3816	T6092	T6091	punct	-,protein
R3817	T6216	T6198	punct	", ",detected
R3818	T6217	T6198	cc	and,detected
R3819	T6218	T6219	advmod	again,detected
R382	T1185	T1184	prep	in,culminate
R3820	T6303	T6286	acomp	relevant,is
R3821	T6219	T6198	conj	detected,detected
R3822	T6220	T6219	punct	", ",detected
R3823	T6221	T6222	det	this,interaction
R3824	T6222	T6219	nsubjpass	interaction,detected
R3825	T6223	T6219	auxpass	was,detected
R3826	T6304	T6303	prep	to,relevant
R3827	T6224	T6219	neg	not,detected
R3828	T6225	T6219	prep	in,detected
R3829	T6226	T6227	det	the,extracts
R383	T1186	T1185	pobj	changes,in
R3830	T6227	T6225	pobj	extracts,in
R3831	T6305	T6306	det	the,state
R3832	T6228	T6227	compound	protein,extracts
R3833	T6229	T6227	prep	from,extracts
R3834	T6230	T6231	compound	control,skin
R3835	T6306	T6304	pobj	state,to
R3836	T6231	T6229	pobj	skin,from
R3837	T6232	T6231	compound	littermate,skin
R3838	T6233	T6234	punct	(,4E
R3839	T6307	T6306	amod	hyperproliferative,state
R384	T1187	T1186	prep	in,changes
R3840	T6234	T6219	parataxis	4E,detected
R3841	T6235	T6234	compound	Figure,4E
R3842	T6236	T6234	punct	),4E
R3843	T6308	T6306	prep	of,state
R3844	T6237	T6198	punct	.,detected
R3845	T6239	T6240	advmod	Together,demonstrate
R3846	T6309	T6310	det	a,keratinocyte
R3847	T6241	T6240	punct	", ",demonstrate
R3848	T6242	T6243	det	these,data
R3849	T6243	T6240	nsubj	data,demonstrate
R385	T1188	T1189	compound	gene,expression
R3850	T6310	T6308	pobj	keratinocyte,of
R3851	T6244	T6245	mark	that,allows
R3852	T6311	T6301	punct	", ",expected
R3853	T6245	T6240	ccomp	allows,demonstrate
R3854	T6246	T6247	det	the,reduction
R3855	T6312	T6301	advmod	then,expected
R3856	T6247	T6245	nsubj	reduction,allows
R3857	T6248	T6247	prep	in,reduction
R3858	T6249	T6250	compound	α,catenin
R3859	T6250	T6252	compound	catenin,levels
R386	T1189	T1187	pobj	expression,in
R3860	T6251	T6250	punct	-,catenin
R3861	T6252	T6248	pobj	levels,in
R3862	T6253	T6247	punct	", ",reduction
R3863	T6313	T6301	nsubjpass	overexpression,expected
R3864	T6254	T6255	preconj	either,by
R3865	T6255	T6247	prep	by,reduction
R3866	T6256	T6257	npadvmod	Snail,mediated
R3867	T6314	T6313	prep	of,overexpression
R3868	T6257	T6259	amod	mediated,regulation
R3869	T6258	T6257	punct	-,mediated
R387	T1190	T1189	punct	", ",expression
R3870	T6259	T6255	pobj	regulation,by
R3871	T6315	T6314	pobj	Ajuba,of
R3872	T6260	T6259	amod	down,regulation
R3873	T6261	T6259	punct	-,regulation
R3874	T6262	T6259	prep	of,regulation
R3875	T6316	T6301	aux	would,expected
R3876	T6263	T6264	compound	E,cadherin
R3877	T6264	T6262	pobj	cadherin,of
R3878	T6265	T6264	punct	-,cadherin
R3879	T6317	T6301	auxpass	be,expected
R388	T1191	T1189	conj	growth,expression
R3880	T6266	T6255	cc	or,by
R3881	T6267	T6255	conj	by,by
R3882	T6268	T6269	compound	α,catenin
R3883	T6318	T6319	aux	to,bypass
R3884	T6269	T6271	npadvmod	catenin,conditional
R3885	T6270	T6269	punct	-,catenin
R3886	T6271	T6272	amod	conditional,targeting
R3887	T6319	T6301	xcomp	bypass,expected
R3888	T6272	T6267	pobj	targeting,by
R3889	T6273	T6245	punct	", ",allows
R389	T1192	T1191	punct	", ",growth
R3890	T6274	T6275	nsubj	Ajuba,interact
R3891	T6320	T6321	det	the,competition
R3892	T6275	T6245	ccomp	interact,allows
R3893	T6276	T6275	aux	to,interact
R3894	T6277	T6275	prep	with,interact
R3895	T6321	T6319	dobj	competition,bypass
R3896	T6278	T6277	pobj	Grb,with
R3897	T6279	T6278	punct	-,Grb
R3898	T6280	T6278	nummod	2,Grb
R3899	T6281	T6278	punct	/,Grb
R39	T191	T192	compound	hair,bud
R390	T1193	T1191	cc	and,growth
R3900	T6322	T6319	cc	and,bypass
R3901	T6282	T6278	appos	Sos,Grb
R3902	T6283	T6240	punct	.,demonstrate
R3903	T6323	T6319	conj	promote,bypass
R3904	T6285	T6286	mark	If,is
R3905	T6286	T6301	advcl	is,expected
R3906	T6287	T6288	det	the,competition
R3907	T6324	T6323	dobj	activation,promote
R3908	T6288	T6286	nsubj	competition,is
R3909	T6289	T6288	prep	between,competition
R391	T1194	T1191	conj	differentiation,growth
R3910	T6290	T6289	pobj	Grb,between
R3911	T6291	T6290	punct	-,Grb
R3912	T6292	T6290	nummod	2,Grb
R3913	T6293	T6290	punct	/,Grb
R3914	T6294	T6290	appos	Sos,Grb
R3915	T6325	T6324	prep	of,activation
R3916	T6295	T6290	cc	and,Grb
R3917	T6296	T6297	compound	α,catenin
R3918	T6297	T6290	conj	catenin,Grb
R3919	T6326	T6327	det	the,pathway
R392	T1195	T1196	punct	[,2
R3920	T6298	T6297	punct	-,catenin
R3921	T6299	T6288	prep	for,competition
R3922	T6300	T6299	pobj	Ajuba,for
R3923	T6327	T6325	pobj	pathway,of
R3924	T6302	T6303	advmod	functionally,relevant
R3925	T6328	T6329	compound	Ras,MAPK
R3926	T6329	T6327	compound	MAPK,pathway
R3927	T6330	T6329	punct	-,MAPK
R3928	T6331	T6324	prep	in,activation
R3929	T6409	T6407	oprd	inh,marked
R393	T1196	T1166	parataxis	2,trigger
R3930	T6410	T6405	punct	”,see
R3931	T6332	T6333	compound	WT,keratinocytes
R3932	T6411	T6402	punct	),4F
R3933	T6412	T6413	punct	[,27
R3934	T6413	T6380	parataxis	27,abolished
R3935	T6333	T6331	pobj	keratinocytes,in
R3936	T6414	T6413	punct	],27
R3937	T6415	T6380	punct	.,abolished
R3938	T6334	T6301	punct	.,expected
R3939	T6417	T6418	poss	Ajuba,domain
R394	T1197	T1196	punct	],2
R3940	T6418	T6421	nsubj	domain,is
R3941	T6419	T6417	case	's,Ajuba
R3942	T6336	T6337	advmod	Indeed,were
R3943	T6420	T6418	amod	pre-LIM,domain
R3944	T6422	T6423	det	the,segment
R3945	T6423	T6421	attr	segment,is
R3946	T6338	T6337	punct	", ",were
R3947	T6424	T6425	dep	that,associates
R3948	T6339	T6340	advmod	when,transfected
R3949	T6425	T6423	relcl	associates,segment
R395	T1198	T1166	punct	.,trigger
R3950	T6426	T6425	prep	with,associates
R3951	T6340	T6337	advcl	transfected,were
R3952	T6427	T6428	poss	Grb,domain
R3953	T6428	T6426	pobj	domain,with
R3954	T6429	T6427	punct	-,Grb
R3955	T6341	T6342	npadvmod	serum,starved
R3956	T6430	T6427	nummod	2,Grb
R3957	T6431	T6427	case	's,Grb
R3958	T6432	T6433	nmod	Src,homology
R3959	T6433	T6428	nmod	homology,domain
R396	T1200	T1201	advmod	How,collaborates
R3960	T6342	T6344	amod	starved,keratinocytes
R3961	T6434	T6433	punct	-,homology
R3962	T6435	T6433	nummod	3,homology
R3963	T6436	T6437	punct	[,9
R3964	T6437	T6421	parataxis	9,is
R3965	T6438	T6437	punct	],9
R3966	T6439	T6421	punct	.,is
R3967	T6343	T6342	punct	-,starved
R3968	T6441	T6442	advmod	When,overexpressed
R3969	T6442	T6446	advcl	overexpressed,observed
R397	T1201	T1206	csubj	collaborates,has
R3970	T6344	T6340	nsubjpass	keratinocytes,transfected
R3971	T6443	T6444	det	this,domain
R3972	T6444	T6442	nsubjpass	domain,overexpressed
R3973	T6445	T6442	auxpass	was,overexpressed
R3974	T6345	T6340	auxpass	were,transfected
R3975	T6447	T6442	prep	in,overexpressed
R3976	T6448	T6449	npadvmod	serum,starved
R3977	T6346	T6340	advmod	transiently,transfected
R3978	T6449	T6451	amod	starved,keratinocytes
R3979	T6450	T6449	punct	-,starved
R398	T1202	T1203	det	this,constellation
R3980	T6451	T6447	pobj	keratinocytes,in
R3981	T6347	T6340	prep	with,transfected
R3982	T6452	T6446	punct	", ",observed
R3983	T6453	T6454	det	a,elevation
R3984	T6454	T6446	nsubjpass	elevation,observed
R3985	T6348	T6349	det	an,vector
R3986	T6455	T6454	amod	comparable,elevation
R3987	T6456	T6454	prep	in,elevation
R3988	T6457	T6456	pobj	pMAPK,in
R3989	T6458	T6446	auxpass	was,observed
R399	T1203	T1201	nsubj	constellation,collaborates
R3990	T6349	T6347	pobj	vector,with
R3991	T6459	T6460	punct	(,4F
R3992	T6460	T6446	parataxis	4F,observed
R3993	T6461	T6460	compound	Figure,4F
R3994	T6350	T6351	compound	Ajuba,expression
R3995	T6462	T6460	punct	),4F
R3996	T6463	T6446	punct	.,observed
R3997	T6351	T6349	compound	expression,vector
R3998	T6465	T6466	mark	As,expected
R3999	T6466	T6467	advcl	expected,blocked
R4	T151	T152	compound	TGF,β2
R40	T192	T193	compound	bud,morphogenesis
R400	T1204	T1203	prep	of,constellation
R4000	T6352	T6337	punct	", ",were
R4001	T6468	T6467	punct	", ",blocked
R4002	T6469	T6470	det	the,inhibitor
R4003	T6470	T6467	nsubj	inhibitor,blocked
R4004	T6471	T6470	amod	small,inhibitor
R4005	T6353	T6354	det	the,levels
R4006	T6472	T6470	compound	peptide,inhibitor
R4007	T6473	T6474	dep	that,interrupts
R4008	T6474	T6470	relcl	interrupts,inhibitor
R4009	T6354	T6337	nsubj	levels,were
R401	T1205	T1204	pobj	signals,of
R4010	T6475	T6476	det	the,association
R4011	T6476	T6474	dobj	association,interrupts
R4012	T6477	T6476	nmod	Grb,association
R4013	T6478	T6477	punct	-,Grb
R4014	T6479	T6477	nummod	2,Grb
R4015	T6355	T6354	prep	of,levels
R4016	T6480	T6477	punct	/,Grb
R4017	T6481	T6477	appos	Sos,Grb
R4018	T6482	T6483	det	the,effects
R4019	T6483	T6467	dobj	effects,blocked
R402	T1207	T1201	prep	in,collaborates
R4020	T6356	T6355	pobj	pMAPK,of
R4021	T6484	T6467	punct	.,blocked
R4022	T6486	T6487	det	These,data
R4023	T6357	T6358	preconj	not,elevated
R4024	T6487	T6488	nsubj	data,suggested
R4025	T6489	T6490	mark	that,associate
R4026	T6358	T6337	acomp	elevated,were
R4027	T6490	T6488	ccomp	associate,suggested
R4028	T6491	T6490	prep	by,associate
R4029	T6492	T6491	pcomp	elevating,by
R403	T1208	T1207	pcomp	tailoring,in
R4030	T6493	T6494	amod	cytosolic,levels
R4031	T6359	T6357	advmod	only,not
R4032	T6494	T6492	dobj	levels,elevating
R4033	T6495	T6494	compound	Ajuba,levels
R4034	T6496	T6490	punct	", ",associate
R4035	T6360	T6358	cc	but,elevated
R4036	T6497	T6498	poss	Ajuba,domain
R4037	T6498	T6490	nsubj	domain,associate
R4038	T6499	T6497	case	's,Ajuba
R4039	T6500	T6498	amod	pre-LIM,domain
R404	T1209	T1210	det	each,process
R4040	T6361	T6360	advmod	also,but
R4041	T6501	T6490	aux	may,associate
R4042	T6502	T6490	prep	with,associate
R4043	T6503	T6502	pobj	Grb,with
R4044	T6362	T6358	conj	comparable,elevated
R4045	T6504	T6503	punct	-,Grb
R4046	T6505	T6503	nummod	2,Grb
R4047	T6506	T6503	punct	/,Grb
R4048	T6363	T6362	prep	to,comparable
R4049	T6507	T6503	appos	Sos,Grb
R405	T1210	T1208	dobj	process,tailoring
R4050	T6508	T6490	prep	in,associate
R4051	T6509	T6510	det	a,manner
R4052	T6364	T6363	pobj	those,to
R4053	T6510	T6508	pobj	manner,in
R4054	T6511	T6512	dep	that,stimulates
R4055	T6365	T6364	acl	transfected,those
R4056	T6512	T6510	relcl	stimulates,manner
R4057	T6513	T6514	poss	its,activity
R4058	T6366	T6365	prep	with,transfected
R4059	T6367	T6368	det	the,transgene
R406	T1211	T1210	compound	budding,process
R4060	T6368	T6366	pobj	transgene,with
R4061	T6514	T6512	dobj	activity,stimulates
R4062	T6515	T6514	compound	nucleotide,activity
R4063	T6516	T6514	compound	exchange,activity
R4064	T6369	T6370	compound	K14,HASnail
R4065	T6517	T6512	cc	and,stimulates
R4066	T6518	T6512	conj	leads,stimulates
R4067	T6370	T6368	compound	HASnail,transgene
R4068	T6519	T6518	prep	to,leads
R4069	T6520	T6519	pobj	activation,to
R407	T1212	T1213	mark	so,executes
R4070	T6521	T6520	prep	of,activation
R4071	T6371	T6370	punct	-,HASnail
R4072	T6522	T6523	det	the,pathway
R4073	T6523	T6521	pobj	pathway,of
R4074	T6524	T6525	compound	Ras,MAPK
R4075	T6372	T6373	punct	(,4F
R4076	T6525	T6523	compound	MAPK,pathway
R4077	T6526	T6525	punct	-,MAPK
R4078	T6527	T6488	punct	.,suggested
R4079	T6373	T6337	parataxis	4F,were
R408	T1213	T1208	advcl	executes,tailoring
R4080	T6529	T6530	mark	Although,provides
R4081	T6530	T6533	advcl	provides,known
R4082	T6374	T6373	compound	Figure,4F
R4083	T6531	T6532	det	this,pathway
R4084	T6532	T6530	nsubj	pathway,provides
R4085	T6375	T6373	punct	),4F
R4086	T6534	T6535	nummod	one,mechanism
R4087	T6535	T6530	dobj	mechanism,provides
R4088	T6536	T6537	prep	by,coupled
R4089	T6537	T6535	relcl	coupled,mechanism
R409	T1214	T1213	mark	that,executes
R4090	T6376	T6337	punct	.,were
R4091	T6538	T6536	pobj	which,by
R4092	T6539	T6540	compound	Snail,expression
R4093	T6540	T6537	nsubjpass	expression,coupled
R4094	T6378	T6379	det	This,activation
R4095	T6541	T6540	cc	and,expression
R4096	T6542	T6540	conj	proliferation,expression
R4097	T6543	T6537	aux	may,coupled
R4098	T6544	T6537	auxpass	be,coupled
R4099	T6379	T6380	nsubjpass	activation,abolished
R41	T193	T189	dobj	morphogenesis,Using
R410	T1215	T1213	nsubj	it,executes
R4100	T6545	T6537	prep	in,coupled
R4101	T6546	T6547	compound	skin,epithelium
R4102	T6547	T6545	pobj	epithelium,in
R4103	T6381	T6380	auxpass	was,abolished
R4104	T6548	T6533	punct	", ",known
R4105	T6549	T6550	amod	proliferative,circuitries
R4106	T6550	T6533	nsubjpass	circuitries,known
R4107	T6551	T6550	acl	involving,circuitries
R4108	T6382	T6383	advmod	when,treated
R4109	T6552	T6551	dobj	AJs,involving
R411	T1216	T1217	det	a,program
R4110	T6553	T6533	auxpass	are,known
R4111	T6554	T6555	aux	to,be
R4112	T6555	T6533	xcomp	be,known
R4113	T6383	T6380	advcl	treated,abolished
R4114	T6556	T6555	acomp	complex,be
R4115	T6557	T6556	cc	and,complex
R4116	T6558	T6559	advmod	often,interwoven
R4117	T6559	T6556	conj	interwoven,complex
R4118	T6560	T6533	punct	.,known
R4119	T6384	T6383	nsubjpass	cells,treated
R412	T1217	T1213	dobj	program,executes
R4120	T6562	T6563	amod	Future,studies
R4121	T6563	T6564	nsubjpass	studies,needed
R4122	T6385	T6383	auxpass	were,treated
R4123	T6565	T6564	aux	will,needed
R4124	T6566	T6564	auxpass	be,needed
R4125	T6567	T6568	aux	to,dissect
R4126	T6386	T6383	prep	with,treated
R4127	T6568	T6564	advcl	dissect,needed
R4128	T6569	T6568	advmod	systematically,dissect
R4129	T6570	T6571	det	these,intricacies
R413	T1218	T1217	amod	distinct,program
R4130	T6387	T6388	det	a,inhibitor
R4131	T6571	T6568	dobj	intricacies,dissect
R4132	T6572	T6571	amod	putative,intricacies
R4133	T6573	T6568	prep	at,dissect
R4134	T6388	T6386	pobj	inhibitor,with
R4135	T6574	T6575	det	a,level
R4136	T6575	T6573	pobj	level,at
R4137	T6576	T6575	amod	molecular,level
R4138	T6389	T6388	amod	small,inhibitor
R4139	T6577	T6564	punct	.,needed
R414	T1219	T1217	amod	morphogenetic,program
R4140	T6390	T6388	compound	peptide,inhibitor
R4141	T6391	T6392	dep	that,interrupts
R4142	T6392	T6388	relcl	interrupts,inhibitor
R4143	T6393	T6392	advmod	specifically,interrupts
R4144	T6394	T6395	det	the,interaction
R4145	T6395	T6392	dobj	interaction,interrupts
R4146	T6396	T6395	nmod	Grb,interaction
R4147	T6397	T6396	punct	-,Grb
R4148	T6398	T6396	nummod	2,Grb
R4149	T6399	T6396	punct	/,Grb
R415	T1220	T1221	advmod	yet,defined
R4150	T6400	T6396	appos	Sos,Grb
R4151	T6401	T6402	punct	(,4F
R4152	T6402	T6380	parataxis	4F,abolished
R4153	T6403	T6402	compound	Figure,4F
R4154	T6404	T6402	punct	;,4F
R4155	T6405	T6402	advcl	see,4F
R4156	T6406	T6405	dobj	lanes,see
R4157	T6407	T6406	acl	marked,lanes
R4158	T6408	T6409	punct	“,inh
R416	T1221	T1206	xcomp	defined,has
R417	T1222	T1221	aux	to,defined
R4172	T7354	T7355	csubj	Probing,Reveals
R4173	T7356	T7357	det	the,Regulation
R4174	T7357	T7354	dobj	Regulation,Probing
R4175	T7358	T7357	prep	of,Regulation
R4176	T7359	T7360	compound	Snail,Gene
R4177	T7360	T7361	compound	Gene,Expression
R4178	T7361	T7358	pobj	Expression,of
R4179	T7362	T7363	det	an,Link
R418	T1223	T1221	auxpass	be,defined
R4180	T7363	T7355	dobj	Link,Reveals
R4181	T7364	T7363	amod	Essential,Link
R4182	T7365	T7363	prep	to,Link
R4183	T7366	T7367	compound	TGF,β2
R4184	T7367	T7369	compound	β2,Signaling
R4185	T7368	T7367	punct	-,β2
R4186	T7369	T7365	pobj	Signaling,to
R4187	T7370	T7355	prep	in,Reveals
R4188	T7371	T7372	det	the,Bud
R4189	T7372	T7370	pobj	Bud,in
R419	T1224	T1221	advmod	comprehensively,defined
R4190	T7373	T7372	amod	Developing,Bud
R4191	T7374	T7372	compound	Hair,Bud
R4192	T7376	T7377	det	The,spike
R4193	T7377	T7379	nsubj	spike,prompted
R4194	T7378	T7377	amod	temporal,spike
R4195	T7380	T7377	prep	of,spike
R4196	T7381	T7382	compound	Snail,expression
R4197	T7382	T7380	pobj	expression,of
R4198	T7383	T7382	compound	mRNA,expression
R4199	T7384	T7377	prep	in,spike
R42	T194	T189	prep	as,Using
R420	T1225	T1206	punct	.,has
R4200	T7385	T7386	det	the,bud
R4201	T7386	T7384	pobj	bud,in
R4202	T7387	T7386	compound	hair,bud
R4203	T7388	T7379	dobj	us,prompted
R4204	T7389	T7390	aux	to,consider
R4205	T7390	T7379	xcomp	consider,prompted
R4206	T7391	T7392	det	what,factor
R4207	T7392	T7393	dep	factor,regulating
R4208	T7393	T7390	ccomp	regulating,consider
R4209	T7394	T7392	punct	(,factor
R421	T1227	T1228	advmod	However,appears
R4210	T7395	T7392	nmod	s,factor
R4211	T7396	T7392	punct	),factor
R4212	T7397	T7393	aux	may,regulating
R4213	T7398	T7393	aux	be,regulating
R4214	T7399	T7400	det	the,gene
R4215	T7400	T7393	dobj	gene,regulating
R4216	T7401	T7400	compound	Snail,gene
R4217	T7402	T7379	punct	.,prompted
R4218	T7404	T7405	det	A,variety
R4219	T7405	T7406	nsubj	variety,have
R422	T1229	T1228	punct	", ",appears
R4220	T7407	T7405	prep	of,variety
R4221	T7408	T7409	amod	extracellular,signals
R4222	T7409	T7407	pobj	signals,of
R4223	T7410	T7411	det	an,impact
R4224	T7411	T7406	dobj	impact,have
R4225	T7412	T7411	prep	on,impact
R4226	T7413	T7414	det	the,expression
R4227	T7414	T7412	pobj	expression,on
R4228	T7415	T7416	compound	cell,type
R4229	T7416	T7417	npadvmod	type,specific
R423	T1230	T1231	det	the,process
R4230	T7417	T7414	amod	specific,expression
R4231	T7418	T7417	punct	-,specific
R4232	T7419	T7414	prep	of,expression
R4233	T7420	T7421	amod	different,members
R4234	T7421	T7419	pobj	members,of
R4235	T7422	T7423	compound	Snail,family
R4236	T7423	T7421	compound	family,members
R4237	T7424	T7406	punct	", ",have
R4238	T7425	T7406	cc	and,have
R4239	T7426	T7427	nsubj	many,affect
R424	T1231	T1228	nsubj	process,appears
R4240	T7427	T7406	conj	affect,have
R4241	T7428	T7426	prep	of,many
R4242	T7429	T7428	pobj	them,of
R4243	T7430	T7426	punct	", ",many
R4244	T7431	T7426	prep	including,many
R4245	T7432	T7431	pobj	Wnts,including
R4246	T7433	T7432	punct	", ",Wnts
R4247	T7434	T7432	conj	BMPs,Wnts
R4248	T7435	T7434	punct	", ",BMPs
R4249	T7436	T7434	conj	FGFs,BMPs
R425	T1232	T1233	aux	to,patterned
R4250	T7437	T7436	punct	", ",FGFs
R4251	T7438	T7436	cc	and,FGFs
R4252	T7439	T7440	compound	TGF,βs
R4253	T7440	T7436	conj	βs,FGFs
R4254	T7441	T7440	punct	-,βs
R4255	T7442	T7427	punct	", ",affect
R4256	T7443	T7427	advmod	also,affect
R4257	T7444	T7445	compound	hair,bud
R4258	T7445	T7446	compound	bud,development
R4259	T7446	T7427	dobj	development,affect
R426	T1233	T1228	xcomp	patterned,appears
R4260	T7447	T7448	punct	[,28
R4261	T7448	T7427	parataxis	28,affect
R4262	T7449	T7448	nummod	2,28
R4263	T7450	T7448	punct	",",28
R4264	T7451	T7448	nummod	16,28
R4265	T7452	T7448	punct	",",28
R4266	T7453	T7448	punct	],28
R4267	T7454	T7427	punct	.,affect
R4268	T7456	T7457	mark	Since,expressed
R4269	T7457	T7461	advcl	expressed,focused
R427	T1234	T1233	auxpass	be,patterned
R4270	T7458	T7457	nsubjpass	Snail,expressed
R4271	T7459	T7457	auxpass	is,expressed
R4272	T7460	T7457	neg	not,expressed
R4273	T7462	T7457	prep	in,expressed
R4274	T7463	T7464	amod	cultured,keratinocytes
R4275	T7464	T7462	pobj	keratinocytes,in
R4276	T7465	T7464	compound	skin,keratinocytes
R4277	T7466	T7467	dep	that,secrete
R4278	T7467	T7464	relcl	secrete,keratinocytes
R4279	T7468	T7469	amod	active,BMPs
R428	T1235	T1233	prep	at,patterned
R4280	T7469	T7467	dobj	BMPs,secrete
R4281	T7470	T7469	cc	and,BMPs
R4282	T7471	T7469	conj	FGFs,BMPs
R4283	T7472	T7473	punct	(,see
R4284	T7473	T7457	parataxis	see,expressed
R4285	T7474	T7475	compound	Figure,1B
R4286	T7475	T7473	dobj	1B,see
R4287	T7476	T7473	punct	),see
R4288	T7477	T7461	punct	", ",focused
R4289	T7478	T7461	nsubj	we,focused
R429	T1236	T1237	det	the,stages
R4290	T7479	T7480	poss	our,attention
R4291	T7480	T7461	dobj	attention,focused
R4292	T7481	T7461	prep	on,focused
R4293	T7482	T7483	nmod	Wnt,signaling
R4294	T7483	T7481	pobj	signaling,on
R4295	T7484	T7482	cc	and,Wnt
R4296	T7485	T7486	compound	TGF,β
R4297	T7486	T7482	conj	β,Wnt
R4298	T7487	T7486	punct	-,β
R4299	T7488	T7483	prep	as,signaling
R43	T195	T196	det	a,paradigm
R430	T1237	T1235	pobj	stages,at
R4300	T7489	T7490	advmod	more,likely
R4301	T7490	T7491	amod	likely,candidates
R4302	T7491	T7488	pobj	candidates,as
R4303	T7492	T7491	prep	for,candidates
R4304	T7493	T7494	compound	Snail,induction
R4305	T7494	T7492	pobj	induction,for
R4306	T7495	T7491	prep	in,candidates
R4307	T7496	T7497	det	this,type
R4308	T7497	T7495	pobj	type,in
R4309	T7498	T7497	compound	cell,type
R431	T1238	T1237	amod	initial,stages
R4310	T7499	T7461	punct	.,focused
R4311	T7501	T7502	advmod	Previously,showed
R4312	T7503	T7502	punct	", ",showed
R4313	T7504	T7502	nsubj	we,showed
R4314	T7505	T7506	mark	that,achieved
R4315	T7506	T7502	ccomp	achieved,showed
R4316	T7507	T7508	amod	effective,transmission
R4317	T7508	T7506	nsubjpass	transmission,achieved
R4318	T7509	T7508	prep	of,transmission
R4319	T7510	T7511	det	a,signal
R432	T1239	T1237	prep	of,stages
R4320	T7511	T7509	pobj	signal,of
R4321	T7512	T7511	nmod	Wnt,signal
R4322	T7513	T7512	punct	-,Wnt
R4323	T7514	T7512	nummod	3a,Wnt
R4324	T7515	T7508	prep	in,transmission
R4325	T7516	T7517	amod	cultured,keratinocytes
R4326	T7517	T7515	pobj	keratinocytes,in
R4327	T7518	T7506	aux	can,achieved
R4328	T7519	T7506	auxpass	be,achieved
R4329	T7520	T7506	prep	through,achieved
R433	T1240	T1241	compound	bud,formation
R4330	T7521	T7522	poss	their,exposure
R4331	T7522	T7520	pobj	exposure,through
R4332	T7523	T7522	prep	to,exposure
R4333	T7524	T7525	det	the,inhibitor
R4334	T7525	T7523	pobj	inhibitor,to
R4335	T7526	T7525	compound	BMP,inhibitor
R4336	T7527	T7525	appos	noggin,inhibitor
R4337	T7528	T7525	punct	", ",inhibitor
R4338	T7529	T7530	dep	which,induces
R4339	T7530	T7525	relcl	induces,inhibitor
R434	T1241	T1239	pobj	formation,of
R4340	T7531	T7532	nmod	LEF,expression
R4341	T7532	T7530	dobj	expression,induces
R4342	T7533	T7531	punct	-,LEF
R4343	T7534	T7531	nummod	1,LEF
R4344	T7535	T7536	punct	[,4
R4345	T7536	T7502	parataxis	4,showed
R4346	T7537	T7536	punct	],4
R4347	T7538	T7502	punct	.,showed
R4348	T7540	T7541	advmod	In,vitro
R4349	T7541	T7542	advmod	vitro,regulated
R435	T1242	T1228	punct	", ",appears
R4350	T7543	T7542	punct	", ",regulated
R4351	T7544	T7545	det	these,conditions
R4352	T7545	T7542	nsubj	conditions,regulated
R4353	T7546	T7542	advmod	down,regulated
R4354	T7547	T7542	punct	-,regulated
R4355	T7548	T7549	det	the,promoter
R4356	T7549	T7542	dobj	promoter,regulated
R4357	T7550	T7551	compound	E,cadherin
R4358	T7551	T7549	compound	cadherin,promoter
R4359	T7552	T7551	punct	-,cadherin
R436	T1243	T1244	mark	since,differ
R4360	T7553	T7542	cc	and,regulated
R4361	T7554	T7542	conj	induced,regulated
R4362	T7555	T7556	det	a,gene
R4363	T7556	T7554	dobj	gene,induced
R4364	T7557	T7558	npadvmod	LEF,sensitive
R4365	T7558	T7556	amod	sensitive,gene
R4366	T7559	T7557	punct	-,LEF
R4367	T7560	T7557	nummod	1,LEF
R4368	T7561	T7557	punct	/,LEF
R4369	T7562	T7563	compound	β,catenin
R437	T1244	T1228	advcl	differ,appears
R4370	T7563	T7557	appos	catenin,LEF
R4371	T7564	T7563	punct	-,catenin
R4372	T7565	T7558	punct	-,sensitive
R4373	T7566	T7556	compound	reporter,gene
R4374	T7567	T7556	punct	", ",gene
R4375	T7568	T7556	appos	TOPFLASH,gene
R4376	T7569	T7570	punct	[,4
R4377	T7570	T7554	parataxis	4,induced
R4378	T7571	T7570	punct	],4
R4379	T7572	T7542	punct	.,regulated
R438	T1245	T1246	det	the,importance
R4380	T7574	T7575	prep	In,induced
R4381	T7576	T7574	pobj	contrast,In
R4382	T7577	T7575	punct	", ",induced
R4383	T7578	T7579	compound	Snail,expression
R4384	T7579	T7575	nsubjpass	expression,induced
R4385	T7580	T7575	auxpass	was,induced
R4386	T7581	T7575	neg	not,induced
R4387	T7582	T7575	agent	by,induced
R4388	T7583	T7584	det	these,conditions
R4389	T7584	T7582	pobj	conditions,by
R439	T1246	T1244	nsubj	importance,differ
R4390	T7585	T7586	punct	(,5A
R4391	T7586	T7575	parataxis	5A,induced
R4392	T7587	T7586	compound	Figure,5A
R4393	T7588	T7586	punct	),5A
R4394	T7589	T7575	punct	.,induced
R4395	T7591	T7592	advmod	Thus,support
R4396	T7593	T7592	punct	", ",support
R4397	T7594	T7592	prep	despite,support
R4398	T7595	T7596	amod	essential,roles
R4399	T7596	T7594	pobj	roles,despite
R44	T196	T194	pobj	paradigm,as
R440	T1247	T1246	amod	relative,importance
R4400	T7597	T7596	prep	for,roles
R4401	T7598	T7597	pobj	Wnts,for
R4402	T7599	T7598	cc	and,Wnts
R4403	T7600	T7598	conj	noggin,Wnts
R4404	T7601	T7596	prep	in,roles
R4405	T7602	T7603	compound	hair,follicle
R4406	T7603	T7604	compound	follicle,specification
R4407	T7604	T7601	pobj	specification,in
R4408	T7605	T7606	punct	[,30
R4409	T7606	T7596	parataxis	30,roles
R441	T1248	T1246	prep	of,importance
R4410	T7607	T7606	nummod	4,30
R4411	T7608	T7606	punct	",",30
R4412	T7609	T7606	nummod	29,30
R4413	T7610	T7606	punct	",",30
R4414	T7611	T7606	punct	],30
R4415	T7612	T7592	punct	", ",support
R4416	T7613	T7614	poss	our,studies
R4417	T7614	T7592	nsubj	studies,support
R4418	T7615	T7592	aux	did,support
R4419	T7616	T7592	neg	not,support
R442	T1249	T1250	det	these,pathways
R4420	T7617	T7618	det	an,role
R4421	T7618	T7592	dobj	role,support
R4422	T7619	T7618	amod	essential,role
R4423	T7620	T7618	prep	for,role
R4424	T7621	T7622	det	these,signals
R4425	T7622	T7620	pobj	signals,for
R4426	T7623	T7618	prep	in,role
R4427	T7624	T7623	pcomp	governing,in
R4428	T7625	T7626	compound	Snail,expression
R4429	T7626	T7624	dobj	expression,governing
R443	T1250	T1248	pobj	pathways,of
R4430	T7627	T7624	prep	in,governing
R4431	T7628	T7627	pobj	keratinocytes,in
R4432	T7629	T7592	punct	.,support
R4433	T7631	T7632	compound	TGF,β1
R4434	T7632	T7634	nsubjpass	β1,shown
R4435	T7633	T7632	punct	-,β1
R4436	T7635	T7634	aux	has,shown
R4437	T7636	T7634	auxpass	been,shown
R4438	T7637	T7638	aux	to,induce
R4439	T7638	T7634	xcomp	induce,shown
R444	T1251	T1250	cc	and,pathways
R4440	T7639	T7640	compound	Snail,members
R4441	T7640	T7638	dobj	members,induce
R4442	T7641	T7640	compound	family,members
R4443	T7642	T7638	prep	in,induce
R4444	T7643	T7642	pobj	hepatocytes,in
R4445	T7644	T7643	cc	and,hepatocytes
R4446	T7645	T7643	conj	heart,hepatocytes
R4447	T7646	T7647	punct	[,31
R4448	T7647	T7634	parataxis	31,shown
R4449	T7648	T7647	nummod	15,31
R445	T1252	T1253	poss	their,effectors
R4450	T7649	T7647	punct	", ",31
R4451	T7650	T7647	punct	],31
R4452	T7651	T7634	punct	.,shown
R4453	T7653	T7654	prep	In,inhibits
R4454	T7655	T7653	pobj	keratinocytes,In
R4455	T7656	T7654	punct	", ",inhibits
R4456	T7657	T7654	advmod	however,inhibits
R4457	T7658	T7654	punct	", ",inhibits
R4458	T7659	T7660	compound	TGF,β1
R4459	T7660	T7654	nsubj	β1,inhibits
R446	T1253	T1250	conj	effectors,pathways
R4460	T7661	T7660	punct	-,β1
R4461	T7662	T7663	compound	keratinocyte,growth
R4462	T7663	T7654	dobj	growth,inhibits
R4463	T7664	T7654	cc	and,inhibits
R4464	T7665	T7654	conj	seems,inhibits
R4465	T7666	T7667	aux	to,involved
R4466	T7667	T7665	xcomp	involved,seems
R4467	T7668	T7667	auxpass	be,involved
R4468	T7669	T7667	prep	in,involved
R4469	T7670	T7669	pcomp	triggering,in
R447	T1254	T1253	amod	downstream,effectors
R4470	T7671	T7672	det	the,phase
R4471	T7672	T7670	dobj	phase,triggering
R4472	T7673	T7672	amod	destructive,phase
R4473	T7674	T7672	prep	of,phase
R4474	T7675	T7676	det	the,follicle
R4475	T7676	T7674	pobj	follicle,of
R4476	T7677	T7676	amod	cycling,follicle
R4477	T7678	T7676	compound	hair,follicle
R4478	T7679	T7680	punct	[,32
R4479	T7680	T7665	parataxis	32,seems
R448	T1255	T1256	mark	as,begin
R4480	T7681	T7680	punct	],32
R4481	T7682	T7654	punct	.,inhibits
R4482	T7684	T7685	prep	Of,blocked
R4483	T7686	T7687	det	the,mutations
R4484	T7687	T7684	pobj	mutations,Of
R4485	T7688	T7687	nmod	loss,mutations
R4486	T7689	T7688	punct	-,loss
R4487	T7690	T7688	prep	of,loss
R4488	T7691	T7690	punct	-,of
R4489	T7692	T7690	pobj	function,of
R449	T1256	T1244	advcl	begin,differ
R4490	T7693	T7687	acl	generated,mutations
R4491	T7694	T7693	prep	in,generated
R4492	T7695	T7694	pobj	each,in
R4493	T7696	T7695	prep	of,each
R4494	T7697	T7698	det	the,genes
R4495	T7698	T7696	pobj	genes,of
R4496	T7699	T7700	compound	TGF,β
R4497	T7700	T7698	compound	β,genes
R4498	T7701	T7700	punct	-,β
R4499	T7702	T7685	punct	", ",blocked
R45	T197	T190	punct	", ",employed
R450	T1257	T1256	nsubj	buds,begin
R4500	T7703	T7704	advmod	only,state
R4501	T7704	T7685	nsubj	state,blocked
R4502	T7705	T7704	det	the,state
R4503	T7706	T7707	nmod	TGF,β2
R4504	T7707	T7704	nmod	β2,state
R4505	T7708	T7707	punct	-,β2
R4506	T7709	T7704	amod	null,state
R4507	T7710	T7711	compound	follicle,development
R4508	T7711	T7685	dobj	development,blocked
R4509	T7712	T7685	prep	at,blocked
R451	T1258	T1259	aux	to,develop
R4510	T7713	T7714	det	the,stage
R4511	T7714	T7712	pobj	stage,at
R4512	T7715	T7716	compound	hair,bud
R4513	T7716	T7714	compound	bud,stage
R4514	T7717	T7718	punct	[,32
R4515	T7718	T7685	parataxis	32,blocked
R4516	T7719	T7718	punct	],32
R4517	T7720	T7685	punct	.,blocked
R4518	T7722	T7723	advmod	Thus,turned
R4519	T7724	T7723	punct	", ",turned
R452	T1259	T1256	xcomp	develop,begin
R4520	T7725	T7723	nsubj	we,turned
R4521	T7726	T7723	prep	towards,turned
R4522	T7727	T7726	pcomp	addressing,towards
R4523	T7728	T7729	mark	whether,involved
R4524	T7729	T7727	ccomp	involved,addressing
R4525	T7730	T7731	compound	TGF,β2
R4526	T7731	T7729	nsubjpass	β2,involved
R4527	T7732	T7731	punct	-,β2
R4528	T7733	T7729	aux	might,involved
R4529	T7734	T7729	auxpass	be,involved
R453	T1260	T1256	cc	and,begin
R4530	T7735	T7729	prep	in,involved
R4531	T7736	T7735	pcomp	regulating,in
R4532	T7737	T7738	compound	Snail,expression
R4533	T7738	T7736	dobj	expression,regulating
R4534	T7739	T7736	prep	in,regulating
R4535	T7740	T7739	pobj	keratinocytes,in
R4536	T7741	T7740	acl	isolated,keratinocytes
R4537	T7742	T7741	prep	from,isolated
R4538	T7743	T7744	det	the,layer
R4539	T7744	T7742	pobj	layer,from
R454	T1261	T1262	compound	cell,fates
R4540	T7745	T7744	amod	basal,layer
R4541	T7746	T7744	prep	of,layer
R4542	T7747	T7748	det	the,epidermis
R4543	T7748	T7746	pobj	epidermis,of
R4544	T7749	T7723	punct	.,turned
R4545	T7751	T7752	mark	Though,is
R4546	T7752	T7754	advcl	is,are
R4547	T7753	T7752	expl	there,is
R4548	T7755	T7756	det	no,system
R4549	T7756	T7752	attr	system,is
R455	T1262	T1263	nsubjpass	fates,specified
R4550	T7757	T7758	compound	cell,culture
R4551	T7758	T7756	compound	culture,system
R4552	T7759	T7756	amod	available,system
R4553	T7760	T7761	aux	to,study
R4554	T7761	T7759	xcomp	study,available
R4555	T7762	T7761	advmod	specifically,study
R4556	T7763	T7764	amod	placodal,cells
R4557	T7764	T7761	dobj	cells,study
R4558	T7765	T7754	punct	", ",are
R4559	T7766	T7767	det	these,keratinocytes
R456	T1263	T1256	conj	specified,begin
R4560	T7767	T7754	nsubj	keratinocytes,are
R4561	T7768	T7769	poss	their,progenitors
R4562	T7769	T7754	attr	progenitors,are
R4563	T7770	T7754	cc	and,are
R4564	T7771	T7754	conj	are,are
R4565	T7772	T7773	det	the,approximation
R4566	T7773	T7771	attr	approximation,are
R4567	T7774	T7773	amod	closest,approximation
R4568	T7775	T7773	amod	available,approximation
R4569	T7776	T7777	aux	to,study
R457	T1264	T1263	auxpass	are,specified
R4570	T7777	T7775	xcomp	study,available
R4571	T7778	T7779	det	the,behavior
R4572	T7779	T7777	dobj	behavior,study
R4573	T7780	T7779	prep	of,behavior
R4574	T7781	T7782	amod	epithelial,cells
R4575	T7782	T7780	pobj	cells,of
R4576	T7783	T7782	prep	of,cells
R4577	T7784	T7785	det	the,placode
R4578	T7785	T7783	pobj	placode,of
R4579	T7786	T7754	punct	.,are
R458	T1265	T1228	punct	.,appears
R4580	T7788	T7789	advmod	Interestingly,caused
R4581	T7790	T7789	punct	", ",caused
R4582	T7791	T7789	nsubj	treatment,caused
R4583	T7792	T7791	prep	of,treatment
R4584	T7793	T7794	amod	cultured,keratinocytes
R4585	T7794	T7792	pobj	keratinocytes,of
R4586	T7795	T7791	prep	with,treatment
R4587	T7796	T7797	advmod	as,5
R4588	T7797	T7800	nummod	5,ng
R4589	T7798	T7797	amod	little,5
R459	T1267	T1268	det	The,development
R4590	T7799	T7797	quantmod	as,5
R4591	T7800	T7795	pobj	ng,with
R4592	T7801	T7802	punct	/,ml
R4593	T7802	T7800	prep	ml,ng
R4594	T7803	T7800	prep	of,ng
R4595	T7804	T7805	compound	TGF,β2
R4596	T7805	T7803	pobj	β2,of
R4597	T7806	T7805	punct	-,β2
R4598	T7807	T7808	det	a,induction
R4599	T7808	T7789	dobj	induction,caused
R46	T198	T190	nsubj	we,employed
R460	T1268	T1269	nsubj	development,requires
R4600	T7809	T7808	amod	rapid,induction
R4601	T7810	T7809	cc	and,rapid
R4602	T7811	T7809	conj	transient,rapid
R4603	T7812	T7808	prep	of,induction
R4604	T7813	T7812	pobj	Snail,of
R4605	T7814	T7815	punct	(,5B
R4606	T7815	T7789	parataxis	5B,caused
R4607	T7816	T7815	compound	Figure,5B
R4608	T7817	T7815	punct	),5B
R4609	T7818	T7789	punct	.,caused
R461	T1270	T1268	prep	of,development
R4610	T7820	T7821	prep	Following,detected
R4611	T7822	T7823	det	this,treatment
R4612	T7823	T7820	pobj	treatment,Following
R4613	T7824	T7821	punct	", ",detected
R4614	T7825	T7826	compound	Snail,protein
R4615	T7826	T7821	nsubjpass	protein,detected
R4616	T7827	T7821	auxpass	was,detected
R4617	T7828	T7821	prep	within,detected
R4618	T7829	T7830	nummod	30,min
R4619	T7830	T7828	pobj	min,within
R462	T1271	T1272	det	a,bud
R4620	T7831	T7821	punct	", ",detected
R4621	T7832	T7821	conj	peaked,detected
R4622	T7833	T7832	prep	at,peaked
R4623	T7834	T7835	nummod	2,h
R4624	T7835	T7833	pobj	h,at
R4625	T7836	T7832	punct	", ",peaked
R4626	T7837	T7832	cc	and,peaked
R4627	T7838	T7839	advmod	then,declined
R4628	T7839	T7832	conj	declined,peaked
R4629	T7840	T7839	advmod	thereafter,declined
R463	T1272	T1270	pobj	bud,of
R4630	T7841	T7821	punct	.,detected
R4631	T7843	T7844	det	The,induction
R4632	T7844	T7845	nsubj	induction,appeared
R4633	T7846	T7844	prep	of,induction
R4634	T7847	T7846	pobj	Snail,of
R4635	T7848	T7849	aux	to,be
R4636	T7849	T7845	xcomp	be,appeared
R4637	T7850	T7849	acomp	specific,be
R4638	T7851	T7850	prep	for,specific
R4639	T7852	T7853	det	the,form
R464	T1273	T1274	det	a,number
R4640	T7853	T7851	pobj	form,for
R4641	T7854	T7853	amod	active,form
R4642	T7855	T7853	prep	of,form
R4643	T7856	T7857	det	the,factor
R4644	T7857	T7855	pobj	factor,of
R4645	T7858	T7857	compound	growth,factor
R4646	T7859	T7849	punct	", ",be
R4647	T7860	T7861	mark	as,obliterated
R4648	T7861	T7849	advcl	obliterated,be
R4649	T7862	T7861	nsubj	pretreatment,obliterated
R465	T1274	T1269	dobj	number,requires
R4650	T7863	T7862	prep	of,pretreatment
R4651	T7864	T7865	compound	TGF,β2
R4652	T7865	T7863	pobj	β2,of
R4653	T7866	T7865	punct	-,β2
R4654	T7867	T7862	prep	for,pretreatment
R4655	T7868	T7869	nummod	10,min
R4656	T7869	T7867	pobj	min,for
R4657	T7870	T7862	prep	at,pretreatment
R4658	T7871	T7872	nummod	100,°C
R4659	T7872	T7870	pobj	°C,at
R466	T1275	T1274	prep	of,number
R4660	T7873	T7874	det	the,response
R4661	T7874	T7861	dobj	response,obliterated
R4662	T7875	T7876	punct	[,lanes
R4663	T7876	T7849	parataxis	lanes,be
R4664	T7877	T7878	compound	Figure,5B
R4665	T7878	T7876	dep	5B,lanes
R4666	T7879	T7876	punct	", ",lanes
R4667	T7880	T7876	acl	marked,lanes
R4668	T7881	T7880	punct	(,marked
R4669	T7882	T7880	oprd	–,marked
R467	T1276	T1277	amod	coordinated,changes
R4670	T7883	T7876	punct	),lanes
R4671	T7884	T7876	punct	],lanes
R4672	T7885	T7845	punct	.,appeared
R4673	T7887	T7888	prep	By,maintained
R4674	T7889	T7887	pobj	contrast,By
R4675	T7890	T7888	punct	", ",maintained
R4676	T7891	T7892	mark	although,remained
R4677	T7892	T7888	advcl	remained,maintained
R4678	T7893	T7894	compound	TGF,β
R4679	T7894	T7896	compound	β,receptor
R468	T1277	T1275	pobj	changes,of
R4680	T7895	T7894	punct	-,β
R4681	T7896	T7897	compound	receptor,activation
R4682	T7897	T7892	nsubj	activation,remained
R4683	T7898	T7892	acomp	elevated,remained
R4684	T7899	T7892	prep	during,remained
R4685	T7900	T7901	det	the,duration
R4686	T7901	T7899	pobj	duration,during
R4687	T7902	T7901	prep	of,duration
R4688	T7903	T7904	det	the,experiment
R4689	T7904	T7902	pobj	experiment,of
R469	T1278	T1277	prep	in,changes
R4690	T7905	T7892	punct	(,remained
R4691	T7906	T7907	mark	as,measured
R4692	T7907	T7892	advcl	measured,remained
R4693	T7908	T7907	prep	by,measured
R4694	T7909	T7910	det	the,phosphorylation
R4695	T7910	T7908	pobj	phosphorylation,by
R4696	T7911	T7910	amod	sustained,phosphorylation
R4697	T7912	T7910	prep	of,phosphorylation
R4698	T7913	T7914	det	the,effector
R4699	T7914	T7912	pobj	effector,of
R47	T199	T200	compound	mutant,mouse
R470	T1279	T1280	det	the,behavior
R4700	T7915	T7914	amod	downstream,effector
R4701	T7916	T7914	appos	SMAD2,effector
R4702	T7917	T7888	punct	),maintained
R4703	T7918	T7919	compound	Snail,expression
R4704	T7919	T7888	nsubjpass	expression,maintained
R4705	T7920	T7888	aux	could,maintained
R4706	T7921	T7888	neg	not,maintained
R4707	T7922	T7888	auxpass	be,maintained
R4708	T7923	T7924	punct	(,5B
R4709	T7924	T7888	parataxis	5B,maintained
R471	T1280	T1278	pobj	behavior,in
R4710	T7925	T7924	compound	Figure,5B
R4711	T7926	T7924	punct	),5B
R4712	T7927	T7888	punct	.,maintained
R4713	T7929	T7930	advmod	Thus,seemed
R4714	T7931	T7930	punct	", ",seemed
R4715	T7932	T7933	mark	although,correlated
R4716	T7933	T7930	advcl	correlated,seemed
R4717	T7934	T7935	compound	Snail,expression
R4718	T7935	T7933	nsubj	expression,correlated
R4719	T7936	T7933	prep	with,correlated
R472	T1281	T1280	prep	of,behavior
R4720	T7937	T7938	amod	phosphorylated,SMAD2
R4721	T7938	T7939	nmod	SMAD2,induction
R4722	T7939	T7936	pobj	induction,with
R4723	T7940	T7938	punct	(,SMAD2
R4724	T7941	T7938	appos	pSMAD2,SMAD2
R4725	T7942	T7939	punct	),induction
R4726	T7943	T7930	punct	", ",seemed
R4727	T7944	T7945	poss	its,decline
R4728	T7945	T7930	nsubj	decline,seemed
R4729	T7946	T7947	aux	to,rely
R473	T1282	T1283	det	the,cells
R4730	T7947	T7930	xcomp	rely,seemed
R4731	T7948	T7947	prep	on,rely
R4732	T7949	T7950	amod	secondary,events
R4733	T7950	T7948	pobj	events,on
R4734	T7951	T7950	amod	downstream,events
R4735	T7952	T7930	punct	.,seemed
R4736	T7954	T7955	det	The,kinetics
R4737	T7955	T7957	nsubjpass	kinetics,reflected
R4738	T7956	T7955	amod	rapid,kinetics
R4739	T7958	T7955	prep	of,kinetics
R474	T1283	T1281	pobj	cells,of
R4740	T7959	T7960	compound	Snail,expression
R4741	T7960	T7958	pobj	expression,of
R4742	T7961	T7957	auxpass	were,reflected
R4743	T7962	T7957	prep	at,reflected
R4744	T7963	T7964	det	the,level
R4745	T7964	T7962	pobj	level,at
R4746	T7965	T7964	compound	mRNA,level
R4747	T7966	T7957	punct	", ",reflected
R4748	T7967	T7957	advcl	suggesting,reflected
R4749	T7968	T7969	mark	that,be
R475	T1284	T1283	amod	targeted,cells
R4750	T7969	T7967	ccomp	be,suggesting
R4751	T7970	T7971	compound	Snail,promoter
R4752	T7971	T7972	compound	promoter,activity
R4753	T7972	T7969	nsubj	activity,be
R4754	T7973	T7972	prep	in,activity
R4755	T7974	T7973	pobj	keratinocytes,in
R4756	T7975	T7969	aux	might,be
R4757	T7976	T7969	acomp	sensitive,be
R4758	T7977	T7976	prep	to,sensitive
R4759	T7978	T7979	compound	TGF,β2
R476	T1285	T1280	prep	within,behavior
R4760	T7979	T7981	compound	β2,signaling
R4761	T7980	T7979	punct	-,β2
R4762	T7981	T7977	pobj	signaling,to
R4763	T7982	T7983	punct	(,5C
R4764	T7983	T7957	parataxis	5C,reflected
R4765	T7984	T7983	compound	Figure,5C
R4766	T7985	T7983	punct	),5C
R4767	T7986	T7957	punct	.,reflected
R4768	T7988	T7989	aux	To,test
R4769	T7989	T7990	advcl	test,engineered
R477	T1286	T1287	det	an,sheet
R4770	T7991	T7992	det	this,possibility
R4771	T7992	T7989	dobj	possibility,test
R4772	T7993	T7990	punct	", ",engineered
R4773	T7994	T7990	nsubj	we,engineered
R4774	T7995	T7996	det	a,transgene
R4775	T7996	T7990	dobj	transgene,engineered
R4776	T7997	T7996	acl	driving,transgene
R4777	T7998	T7999	det	the,reporter
R4778	T7999	T7997	dobj	reporter,driving
R4779	T8000	T8001	compound	β,galactosidase
R478	T1287	T1285	pobj	sheet,within
R4780	T8001	T7999	compound	galactosidase,reporter
R4781	T8002	T8001	punct	-,galactosidase
R4782	T8003	T7997	prep	under,driving
R4783	T8004	T8005	det	the,control
R4784	T8005	T8003	pobj	control,under
R4785	T8006	T8005	prep	of,control
R4786	T8007	T8008	advmod	approximately,2.2
R4787	T8008	T8009	nummod	2.2,kb
R4788	T8009	T8006	pobj	kb,of
R4789	T8010	T8009	prep	of,kb
R479	T1288	T1287	amod	epithelial,sheet
R4790	T8011	T8012	compound	promoter,sequence
R4791	T8012	T8010	pobj	sequence,of
R4792	T8013	T8012	acl	located,sequence
R4793	T8014	T8013	npadvmod	5,located
R4794	T8015	T8014	punct	′,5
R4795	T8016	T8014	prep	from,5
R4796	T8017	T8018	det	the,site
R4797	T8018	T8016	pobj	site,from
R4798	T8019	T8018	compound	transcription,site
R4799	T8020	T8018	compound	initiation,site
R48	T200	T201	compound	mouse,models
R480	T1289	T1269	punct	.,requires
R4800	T8021	T8018	prep	of,site
R4801	T8022	T8023	det	the,gene
R4802	T8023	T8021	pobj	gene,of
R4803	T8024	T8023	compound	mouse,gene
R4804	T8025	T8023	compound	Snail,gene
R4805	T8026	T7990	punct	.,engineered
R4806	T8028	T8029	prep	At,treated
R4807	T8030	T8031	nummod	2,d
R4808	T8031	T8028	pobj	d,At
R4809	T8032	T8031	prep	after,d
R481	T1291	T1292	det	The,process
R4810	T8033	T8034	amod	transient,transfection
R4811	T8034	T8032	pobj	transfection,after
R4812	T8035	T8029	punct	", ",treated
R4813	T8036	T8029	nsubjpass	keratinocytes,treated
R4814	T8037	T8029	auxpass	were,treated
R4815	T8038	T8029	prep	with,treated
R4816	T8039	T8040	compound	TGF,β2
R4817	T8040	T8038	pobj	β2,with
R4818	T8041	T8040	punct	-,β2
R4819	T8042	T8043	punct	(,0
R482	T1292	T1293	nsubjpass	process,accompanied
R4820	T8043	T8029	parataxis	0,treated
R4821	T8044	T8043	nsubj	t,0
R4822	T8045	T8043	punct	=,0
R4823	T8046	T8043	punct	),0
R4824	T8047	T8029	cc	and,treated
R4825	T8048	T8049	advmod	then,assayed
R4826	T8049	T8029	conj	assayed,treated
R4827	T8050	T8049	prep	for,assayed
R4828	T8051	T8052	compound	transgene,activity
R4829	T8052	T8050	pobj	activity,for
R483	T1294	T1293	aux	must,accompanied
R4830	T8053	T8049	prep	over,assayed
R4831	T8054	T8055	det	the,course
R4832	T8055	T8053	pobj	course,over
R4833	T8056	T8055	amod	same,course
R4834	T8057	T8055	compound	time,course
R4835	T8058	T8059	prep	in,observed
R4836	T8059	T8055	relcl	observed,course
R4837	T8060	T8058	pobj	which,in
R4838	T8061	T8059	nsubj	we,observed
R4839	T8062	T8059	aux	had,observed
R484	T1295	T1293	auxpass	be,accompanied
R4840	T8063	T8064	compound	Snail,protein
R4841	T8064	T8065	compound	protein,induction
R4842	T8065	T8059	dobj	induction,observed
R4843	T8066	T8029	punct	.,treated
R4844	T8068	T8069	det	The,results
R4845	T8069	T8070	nsubjpass	results,presented
R4846	T8071	T8069	prep	of,results
R4847	T8072	T8073	det	this,experiment
R4848	T8073	T8071	pobj	experiment,of
R4849	T8074	T8070	auxpass	are,presented
R485	T1296	T1293	prep	by,accompanied
R4850	T8075	T8070	prep	in,presented
R4851	T8076	T8077	compound	Figure,5D
R4852	T8077	T8075	pobj	5D,in
R4853	T8078	T8070	punct	.,presented
R4854	T8080	T8081	prep	Within,increased
R4855	T8082	T8083	nummod	0.5,h
R4856	T8083	T8080	pobj	h,Within
R4857	T8084	T8083	prep	of,h
R4858	T8085	T8086	compound	TGF,β2
R4859	T8086	T8088	compound	β2,treatment
R486	T1297	T1296	pobj	alterations,by
R4860	T8087	T8086	punct	-,β2
R4861	T8088	T8084	pobj	treatment,of
R4862	T8089	T8081	punct	", ",increased
R4863	T8090	T8091	compound	Snail,promoter
R4864	T8091	T8092	compound	promoter,activity
R4865	T8092	T8081	nsubj	activity,increased
R4866	T8093	T8081	aux	had,increased
R4867	T8094	T8081	advmod	3-fold,increased
R4868	T8095	T8081	punct	", ",increased
R4869	T8096	T8081	cc	and,increased
R487	T1298	T1297	prep	in,alterations
R4870	T8097	T8098	prep	by,peaked
R4871	T8098	T8081	conj	peaked,increased
R4872	T8099	T8100	nummod	2,h
R4873	T8100	T8097	pobj	h,by
R4874	T8101	T8098	punct	", ",peaked
R4875	T8102	T8098	nsubj	it,peaked
R4876	T8103	T8098	prep	to,peaked
R4877	T8104	T8105	advmod	approximately,10-fold
R4878	T8105	T8103	pcomp	10-fold,to
R4879	T8106	T8105	prep	over,10-fold
R488	T1299	T1300	det	the,proliferation
R4880	T8107	T8108	compound	control,levels
R4881	T8108	T8106	pobj	levels,over
R4882	T8109	T8110	punct	(,5D
R4883	T8110	T8098	parataxis	5D,peaked
R4884	T8111	T8110	compound	Figure,5D
R4885	T8112	T8110	punct	),5D
R4886	T8113	T8098	punct	.,peaked
R4887	T8115	T8116	advmod	Thereafter,returned
R4888	T8117	T8116	punct	", ",returned
R4889	T8118	T8119	compound	Snail,promoter
R489	T1300	T1298	pobj	proliferation,in
R4890	T8119	T8120	compound	promoter,activity
R4891	T8120	T8116	nsubj	activity,returned
R4892	T8121	T8116	advmod	rapidly,returned
R4893	T8122	T8116	prep	to,returned
R4894	T8123	T8124	det	the,levels
R4895	T8124	T8122	pobj	levels,to
R4896	T8125	T8124	amod	basal,levels
R4897	T8126	T8124	acl	seen,levels
R4898	T8127	T8126	prep	in,seen
R4899	T8128	T8129	amod	unstimulated,keratinocytes
R49	T201	T190	dobj	models,employed
R490	T1301	T1300	punct	", ",proliferation
R4900	T8129	T8127	pobj	keratinocytes,in
R4901	T8130	T8116	punct	.,returned
R4902	T8132	T8133	det	The,kinetics
R4903	T8133	T8134	nsubj	kinetics,paralleled
R4904	T8135	T8133	prep	of,kinetics
R4905	T8136	T8137	compound	Snail,promoter
R4906	T8137	T8138	compound	promoter,activity
R4907	T8138	T8135	pobj	activity,of
R4908	T8139	T8134	advmod	closely,paralleled
R4909	T8140	T8134	dobj	those,paralleled
R491	T1302	T1300	conj	polarity,proliferation
R4910	T8141	T8140	acl	observed,those
R4911	T8142	T8141	prep	for,observed
R4912	T8143	T8144	compound	Snail,protein
R4913	T8144	T8145	compound	protein,induction
R4914	T8145	T8142	pobj	induction,for
R4915	T8146	T8134	punct	.,paralleled
R4916	T8148	T8149	advmod	Moreover,appeared
R4917	T8150	T8149	punct	", ",appeared
R4918	T8151	T8152	det	the,effects
R4919	T8152	T8149	nsubj	effects,appeared
R492	T1303	T1302	punct	", ",polarity
R4920	T8153	T8152	amod	stimulatory,effects
R4921	T8154	T8155	aux	to,be
R4922	T8155	T8149	xcomp	be,appeared
R4923	T8156	T8155	acomp	specific,be
R4924	T8157	T8156	prep	to,specific
R4925	T8158	T8159	compound	TGF,β2
R4926	T8159	T8157	pobj	β2,to
R4927	T8160	T8159	punct	-,β2
R4928	T8161	T8155	punct	", ",be
R4929	T8162	T8163	mark	since,abrogated
R493	T1304	T1302	conj	shape,polarity
R4930	T8163	T8155	advcl	abrogated,be
R4931	T8164	T8163	nsubjpass	they,abrogated
R4932	T8165	T8163	auxpass	were,abrogated
R4933	T8166	T8167	preconj	either,by
R4934	T8167	T8163	prep	by,abrogated
R4935	T8168	T8169	compound	heat,inactivation
R4936	T8169	T8167	pobj	inactivation,by
R4937	T8170	T8169	prep	of,inactivation
R4938	T8171	T8172	det	the,protein
R4939	T8172	T8170	pobj	protein,of
R494	T1305	T1304	punct	", ",shape
R4940	T8173	T8174	compound	TGF,β2
R4941	T8174	T8172	compound	β2,protein
R4942	T8175	T8174	punct	-,β2
R4943	T8176	T8167	cc	or,by
R4944	T8177	T8167	conj	by,by
R4945	T8178	T8177	pobj	mutation,by
R4946	T8179	T8178	prep	of,mutation
R4947	T8180	T8181	det	a,element
R4948	T8181	T8179	pobj	element,of
R4949	T8182	T8181	amod	putative,element
R495	T1306	T1304	cc	and,shape
R4950	T8183	T8184	compound	SMAD,binding
R4951	T8184	T8181	compound	binding,element
R4952	T8185	T8181	acl	located,element
R4953	T8186	T8187	quantmod	about,1.8
R4954	T8187	T8188	nummod	1.8,kb
R4955	T8188	T8185	npadvmod	kb,located
R4956	T8189	T8188	nummod	5,kb
R4957	T8190	T8188	punct	′,kb
R4958	T8191	T8188	prep	from,kb
R4959	T8192	T8193	det	the,site
R496	T1307	T1304	conj	adhesiveness,shape
R4960	T8193	T8191	pobj	site,from
R4961	T8194	T8193	compound	Snail,site
R4962	T8195	T8193	compound	transcription,site
R4963	T8196	T8193	compound	start,site
R4964	T8197	T8198	punct	(,5D
R4965	T8198	T8163	parataxis	5D,abrogated
R4966	T8199	T8198	compound	Figure,5D
R4967	T8200	T8198	punct	),5D
R4968	T8201	T8149	punct	.,appeared
R4969	T8203	T8204	advcl	Taken,suggested
R497	T1308	T1300	prep	of,proliferation
R4970	T8205	T8203	advmod	together,Taken
R4971	T8206	T8204	punct	", ",suggested
R4972	T8207	T8208	det	these,results
R4973	T8208	T8204	nsubj	results,suggested
R4974	T8209	T8210	mark	that,results
R4975	T8210	T8204	advcl	results,suggested
R4976	T8211	T8210	prep	in,results
R4977	T8212	T8211	pobj	keratinocytes,in
R4978	T8213	T8210	punct	", ",results
R4979	T8214	T8215	compound	TGF,β2
R498	T1309	T1310	amod	selected,cells
R4980	T8215	T8217	compound	β2,signaling
R4981	T8216	T8215	punct	-,β2
R4982	T8217	T8210	nsubj	signaling,results
R4983	T8218	T8210	prep	in,results
R4984	T8219	T8220	det	a,activation
R4985	T8220	T8218	pobj	activation,in
R4986	T8221	T8222	npadvmod	pSMAD2,dependent
R4987	T8222	T8220	amod	dependent,activation
R4988	T8223	T8222	punct	-,dependent
R4989	T8224	T8220	amod	transient,activation
R499	T1310	T1308	pobj	cells,of
R4990	T8225	T8220	prep	of,activation
R4991	T8226	T8227	det	the,gene
R4992	T8227	T8225	pobj	gene,of
R4993	T8228	T8227	compound	Snail,gene
R4994	T8229	T8210	punct	", ",results
R4995	T8230	T8210	cc	and,results
R4996	T8231	T8232	mark	that,relies
R4997	T8232	T8210	conj	relies,results
R4998	T8233	T8232	nsubj	maintenance,relies
R4999	T8234	T8233	prep	of,maintenance
R5	T152	T150	dobj	β2,Involving
R50	T202	T201	cc	and,models
R500	T1311	T1296	punct	", ",by
R5000	T8235	T8236	compound	Snail,protein
R5001	T8236	T8234	pobj	protein,of
R5002	T8237	T8232	punct	", ",relies
R5003	T8238	T8232	prep	in,relies
R5004	T8239	T8238	pobj	part,in
R5005	T8240	T8232	punct	", ",relies
R5006	T8241	T8232	prep	upon,relies
R5007	T8242	T8243	amod	sustained,activity
R5008	T8243	T8241	pobj	activity,upon
R5009	T8244	T8243	compound	promoter,activity
R501	T1312	T1313	advmod	as,as
R5010	T8245	T8204	punct	.,suggested
R5011	T8247	T8248	det	The,brevity
R5012	T8248	T8249	nsubj	brevity,resembled
R5013	T8250	T8248	prep	of,brevity
R5014	T8251	T8252	nmod	Snail,gene
R5015	T8252	T8253	nmod	gene,induction
R5016	T8253	T8250	pobj	induction,of
R5017	T8254	T8252	cc	and,gene
R5018	T8255	T8252	conj	protein,gene
R5019	T8256	T8253	prep	in,induction
R502	T1313	T1296	cc	as,by
R5020	T8257	T8258	compound	TGF,β2
R5021	T8258	T8260	npadvmod	β2,treated
R5022	T8259	T8258	punct	-,β2
R5023	T8260	T8261	amod	treated,keratinocytes
R5024	T8261	T8256	pobj	keratinocytes,in
R5025	T8262	T8261	amod	cultured,keratinocytes
R5026	T8263	T8264	det	the,appearance
R5027	T8264	T8249	dobj	appearance,resembled
R5028	T8265	T8264	amod	temporal,appearance
R5029	T8266	T8264	prep	of,appearance
R503	T1314	T1313	advmod	well,as
R5030	T8267	T8268	compound	Snail,mRNA
R5031	T8268	T8266	pobj	mRNA,of
R5032	T8269	T8268	cc	and,mRNA
R5033	T8270	T8268	conj	protein,mRNA
R5034	T8271	T8264	prep	at,appearance
R5035	T8272	T8273	det	the,initiation
R5036	T8273	T8271	pobj	initiation,at
R5037	T8274	T8273	prep	of,initiation
R5038	T8275	T8276	compound	hair,follicle
R5039	T8276	T8277	compound	follicle,morphogenesis
R504	T1315	T1296	conj	by,by
R5040	T8277	T8274	pobj	morphogenesis,of
R5041	T8278	T8249	prep	in,resembled
R5042	T8279	T8280	amod	embryonic,skin
R5043	T8280	T8278	pobj	skin,in
R5044	T8281	T8280	compound	mouse,skin
R5045	T8282	T8249	punct	.,resembled
R5046	T8284	T8285	aux	To,test
R5047	T8285	T8286	advcl	test,analyzed
R5048	T8287	T8288	mark	whether,required
R5049	T8288	T8285	ccomp	required,test
R505	T1316	T1315	pobj	modifications,by
R5050	T8289	T8290	compound	TGF,β2
R5051	T8290	T8288	nsubjpass	β2,required
R5052	T8291	T8290	punct	-,β2
R5053	T8292	T8288	aux	might,required
R5054	T8293	T8288	auxpass	be,required
R5055	T8294	T8288	prep	for,required
R5056	T8295	T8296	compound	Snail,induction
R5057	T8296	T8294	pobj	induction,for
R5058	T8297	T8296	prep	in,induction
R5059	T8298	T8299	compound	hair,bud
R506	T1317	T1316	prep	in,modifications
R5060	T8299	T8300	compound	bud,formation
R5061	T8300	T8297	pobj	formation,in
R5062	T8301	T8302	advmod	in,vivo
R5063	T8302	T8288	advmod	vivo,required
R5064	T8303	T8286	punct	", ",analyzed
R5065	T8304	T8286	nsubj	we,analyzed
R5066	T8305	T8286	advmod	first,analyzed
R5067	T8306	T8307	mark	whether,expressed
R5068	T8307	T8286	ccomp	expressed,analyzed
R5069	T8308	T8309	compound	TGF,β2
R507	T1318	T1319	poss	their,lamina
R5070	T8309	T8307	nsubjpass	β2,expressed
R5071	T8310	T8309	punct	-,β2
R5072	T8311	T8307	auxpass	was,expressed
R5073	T8312	T8307	prep	in,expressed
R5074	T8313	T8312	cc	or,in
R5075	T8314	T8312	conj	around,in
R5076	T8315	T8316	det	the,bud
R5077	T8316	T8314	pobj	bud,around
R5078	T8317	T8316	compound	hair,bud
R5079	T8318	T8286	punct	.,analyzed
R508	T1319	T1317	pobj	lamina,in
R5080	T8320	T8321	advcl	Consistent,labeled
R5081	T8322	T8320	prep	with,Consistent
R5082	T8323	T8324	amod	previous,observations
R5083	T8324	T8322	pobj	observations,with
R5084	T8325	T8326	punct	[,33
R5085	T8326	T8320	parataxis	33,Consistent
R5086	T8327	T8326	punct	],33
R5087	T8328	T8321	punct	", ",labeled
R5088	T8329	T8330	det	an,antibody
R5089	T8330	T8321	nsubj	antibody,labeled
R509	T1320	T1319	amod	underlying,lamina
R5090	T8331	T8332	amod	anti-TGF,β2
R5091	T8332	T8330	compound	β2,antibody
R5092	T8333	T8332	punct	-,β2
R5093	T8334	T8335	amod	developing,buds
R5094	T8335	T8321	dobj	buds,labeled
R5095	T8336	T8335	compound	hair,buds
R5096	T8337	T8338	punct	(,6A
R5097	T8338	T8321	parataxis	6A,labeled
R5098	T8339	T8338	compound	Figure,6A
R5099	T8340	T8338	punct	),6A
R51	T203	T204	amod	cultured,keratinocytes
R510	T1321	T1319	amod	basal,lamina
R5100	T8341	T8321	punct	.,labeled
R5101	T8343	T8344	det	This,labeling
R5102	T8344	T8345	nsubj	labeling,appeared
R5103	T8346	T8347	aux	to,be
R5104	T8347	T8345	xcomp	be,appeared
R5105	T8348	T8347	acomp	specific,be
R5106	T8349	T8350	mark	as,judged
R5107	T8350	T8347	advcl	judged,be
R5108	T8351	T8350	prep	by,judged
R5109	T8352	T8353	det	the,lack
R511	T1322	T1293	punct	.,accompanied
R5110	T8353	T8351	pobj	lack,by
R5111	T8354	T8353	prep	of,lack
R5112	T8355	T8354	pobj	staining,of
R5113	T8356	T8353	prep	in,lack
R5114	T8357	T8358	compound	follicle,buds
R5115	T8358	T8356	pobj	buds,in
R5116	T8359	T8358	prep	from,buds
R5117	T8360	T8359	pobj	mice,from
R5118	T8361	T8360	amod	homozygous,mice
R5119	T8362	T8361	prep	for,homozygous
R512	T1324	T1325	advmod	Thus,orchestrated
R5120	T8363	T8364	det	a,mutation
R5121	T8364	T8362	pobj	mutation,for
R5122	T8365	T8366	nmod	TGF,β2
R5123	T8366	T8364	nmod	β2,mutation
R5124	T8367	T8366	punct	-,β2
R5125	T8368	T8364	amod	null,mutation
R5126	T8369	T8370	punct	(,34
R5127	T8370	T8345	parataxis	34,appeared
R5128	T8371	T8372	compound	Figure,6A
R5129	T8372	T8370	dep	6A,34
R513	T1326	T1325	punct	", ",orchestrated
R5130	T8373	T8370	punct	;,34
R5131	T8374	T8370	punct	[,34
R5132	T8375	T8370	punct	],34
R5133	T8376	T8370	punct	),34
R5134	T8377	T8345	punct	.,appeared
R5135	T8379	T8380	advmod	Moreover,expressed
R5136	T8381	T8380	punct	", ",expressed
R5137	T8382	T8383	det	the,effector
R5138	T8383	T8380	nsubjpass	effector,expressed
R5139	T8384	T8383	amod	downstream,effector
R514	T1327	T1328	amod	extracellular,crosstalk
R5140	T8385	T8383	prep	of,effector
R5141	T8386	T8387	compound	TGF,β2
R5142	T8387	T8389	compound	β2,signaling
R5143	T8388	T8387	punct	-,β2
R5144	T8389	T8385	pobj	signaling,of
R5145	T8390	T8383	punct	", ",effector
R5146	T8391	T8383	appos	pSMAD2,effector
R5147	T8392	T8380	punct	", ",expressed
R5148	T8393	T8380	auxpass	was,expressed
R5149	T8394	T8380	advmod	also,expressed
R515	T1328	T1325	nsubjpass	crosstalk,orchestrated
R5150	T8395	T8380	prep	in,expressed
R5151	T8396	T8397	nmod	WT,buds
R5152	T8397	T8395	pobj	buds,in
R5153	T8398	T8396	punct	", ",WT
R5154	T8399	T8396	cc	but,WT
R5155	T8400	T8399	neg	not,but
R5156	T8401	T8402	compound	TGF,β2
R5157	T8402	T8404	npadvmod	β2,null
R5158	T8403	T8402	punct	-,β2
R5159	T8404	T8396	conj	null,WT
R516	T1329	T1330	amod	epithelial,mesenchymal
R5160	T8405	T8404	punct	-,null
R5161	T8406	T8397	punct	", ",buds
R5162	T8407	T8397	compound	hair,buds
R5163	T8408	T8409	punct	(,6B
R5164	T8409	T8380	parataxis	6B,expressed
R5165	T8410	T8409	compound	Figure,6B
R5166	T8411	T8409	punct	),6B
R5167	T8412	T8380	punct	.,expressed
R5168	T8414	T8415	advmod	Together,underscore
R5169	T8416	T8415	punct	", ",underscore
R517	T1330	T1328	amod	mesenchymal,crosstalk
R5170	T8417	T8418	det	these,data
R5171	T8418	T8415	nsubj	data,underscore
R5172	T8419	T8420	det	the,importance
R5173	T8420	T8415	dobj	importance,underscore
R5174	T8421	T8420	prep	of,importance
R5175	T8422	T8423	det	the,isoform
R5176	T8423	T8421	pobj	isoform,of
R5177	T8424	T8425	compound	TGF,β2
R5178	T8425	T8423	compound	β2,isoform
R5179	T8426	T8425	punct	-,β2
R518	T1331	T1330	punct	-,mesenchymal
R5180	T8427	T8415	prep	despite,underscore
R5181	T8428	T8427	pobj	expression,despite
R5182	T8429	T8428	prep	of,expression
R5183	T8430	T8431	preconj	both,β1
R5184	T8431	T8429	pobj	β1,of
R5185	T8432	T8431	compound	TGF,β1
R5186	T8433	T8431	punct	-,β1
R5187	T8434	T8431	cc	and,β1
R5188	T8435	T8436	compound	TGF,β2
R5189	T8436	T8431	conj	β2,β1
R519	T1332	T1325	aux	must,orchestrated
R5190	T8437	T8436	punct	-,β2
R5191	T8438	T8428	prep	in,expression
R5192	T8439	T8440	amod	developing,buds
R5193	T8440	T8438	pobj	buds,in
R5194	T8441	T8440	compound	hair,buds
R5195	T8442	T8428	prep	at,expression
R5196	T8443	T8444	det	this,stage
R5197	T8444	T8442	pobj	stage,at
R5198	T8445	T8415	punct	.,underscore
R5199	T8447	T8448	aux	To,explore
R52	T204	T201	conj	keratinocytes,models
R520	T1333	T1325	auxpass	be,orchestrated
R5200	T8448	T8450	advcl	explore,examined
R5201	T8449	T8448	advmod	further,explore
R5202	T8451	T8452	det	the,relation
R5203	T8452	T8448	dobj	relation,explore
R5204	T8453	T8452	amod	possible,relation
R5205	T8454	T8452	prep	between,relation
R5206	T8455	T8454	pobj	Snail,between
R5207	T8456	T8455	cc	and,Snail
R5208	T8457	T8458	compound	TGF,β2
R5209	T8458	T8455	conj	β2,Snail
R521	T1334	T1325	advmod	intricately,orchestrated
R5210	T8459	T8458	punct	-,β2
R5211	T8460	T8450	punct	", ",examined
R5212	T8461	T8450	nsubj	we,examined
R5213	T8462	T8463	det	the,status
R5214	T8463	T8450	dobj	status,examined
R5215	T8464	T8463	prep	of,status
R5216	T8465	T8466	compound	Snail,expression
R5217	T8466	T8464	pobj	expression,of
R5218	T8467	T8463	prep	in,status
R5219	T8468	T8469	compound	TGF,β2
R522	T1335	T1336	aux	to,couple
R5220	T8469	T8471	npadvmod	β2,null
R5221	T8470	T8469	punct	-,β2
R5222	T8471	T8473	amod	null,buds
R5223	T8472	T8471	punct	-,null
R5224	T8473	T8467	pobj	buds,in
R5225	T8474	T8473	compound	hair,buds
R5226	T8475	T8450	punct	.,examined
R5227	T8477	T8478	mark	As,judged
R5228	T8478	T8479	advcl	judged,was
R5229	T8480	T8478	prep	by,judged
R523	T1336	T1325	advcl	couple,orchestrated
R5230	T8481	T8480	pobj	immunohistochemistry,by
R5231	T8482	T8479	punct	", ",was
R5232	T8483	T8484	compound	Snail,protein
R5233	T8484	T8479	nsubj	protein,was
R5234	T8485	T8479	acomp	absent,was
R5235	T8486	T8485	prep	from,absent
R5236	T8487	T8488	compound	E17.5,skin
R5237	T8488	T8486	pobj	skin,from
R5238	T8489	T8488	prep	of,skin
R5239	T8490	T8491	compound	TGF,β2
R524	T1337	T1338	det	the,determination
R5240	T8491	T8493	npadvmod	β2,null
R5241	T8492	T8491	punct	-,β2
R5242	T8493	T8495	amod	null,embryos
R5243	T8494	T8493	punct	-,null
R5244	T8495	T8489	pobj	embryos,of
R5245	T8496	T8486	cc	but,from
R5246	T8497	T8496	neg	not,but
R5247	T8498	T8486	conj	from,from
R5248	T8499	T8498	pobj	that,from
R5249	T8500	T8499	prep	of,that
R525	T1338	T1336	dobj	determination,couple
R5250	T8501	T8502	compound	control,littermates
R5251	T8502	T8500	pobj	littermates,of
R5252	T8503	T8504	punct	(,6C
R5253	T8504	T8479	parataxis	6C,was
R5254	T8505	T8504	compound	Figure,6C
R5255	T8506	T8504	punct	),6C
R5256	T8507	T8479	punct	.,was
R5257	T8509	T8510	det	This,effect
R5258	T8510	T8511	nsubj	effect,appeared
R5259	T8512	T8513	aux	to,exerted
R526	T1339	T1338	prep	of,determination
R5260	T8513	T8511	xcomp	exerted,appeared
R5261	T8514	T8513	auxpass	be,exerted
R5262	T8515	T8513	prep	at,exerted
R5263	T8516	T8517	det	the,level
R5264	T8517	T8515	pobj	level,at
R5265	T8518	T8517	amod	transcriptional,level
R5266	T8519	T8511	punct	", ",appeared
R5267	T8520	T8521	mark	since,found
R5268	T8521	T8511	advcl	found,appeared
R5269	T8522	T8523	compound	Snail,mRNAs
R527	T1340	T1341	amod	distinct,fates
R5270	T8523	T8521	nsubjpass	mRNAs,found
R5271	T8524	T8521	auxpass	were,found
R5272	T8525	T8521	advmod	also,found
R5273	T8526	T8521	neg	not,found
R5274	T8527	T8521	prep	in,found
R5275	T8528	T8529	compound	TGF,β2
R5276	T8529	T8531	npadvmod	β2,null
R5277	T8530	T8529	punct	-,β2
R5278	T8531	T8532	amod	null,buds
R5279	T8532	T8527	pobj	buds,in
R528	T1341	T1339	pobj	fates,of
R5280	T8533	T8532	compound	hair,buds
R5281	T8534	T8521	prep	under,found
R5282	T8535	T8534	pobj	conditions,under
R5283	T8536	T8537	prep	in,detected
R5284	T8537	T8535	relcl	detected,conditions
R5285	T8538	T8536	pobj	which,in
R5286	T8539	T8540	det	the,signal
R5287	T8559	T8557	prep	in,detected
R5288	T8540	T8537	nsubjpass	signal,detected
R5289	T8541	T8537	auxpass	was,detected
R529	T1342	T1341	compound	cell,fates
R5290	T8560	T8561	nummod	2,wk
R5291	T8561	T8563	npadvmod	wk,old
R5292	T8542	T8537	advmod	readily,detected
R5293	T8562	T8561	punct	-,wk
R5294	T8563	T8565	amod	old,mice
R5295	T8564	T8563	punct	-,old
R5296	T8543	T8537	prep	in,detected
R5297	T8565	T8559	pobj	mice,in
R5298	T8566	T8567	compound	K14,Smad2
R5299	T8567	T8565	compound	Smad2,mice
R53	T205	T206	aux	to,dissect
R530	T1343	T1336	prep	with,couple
R5300	T8568	T8567	punct	-,Smad2
R5301	T8569	T8565	compound	Tg,mice
R5302	T8544	T8545	det	the,buds
R5303	T8570	T8565	punct	", ",mice
R5304	T8571	T8572	dep	which,display
R5305	T8572	T8565	relcl	display,mice
R5306	T8545	T8543	pobj	buds,in
R5307	T8573	T8574	amod	elevated,signaling
R5308	T8546	T8545	compound	hair,buds
R5309	T8574	T8572	dobj	signaling,display
R531	T1344	T1345	det	the,remodeling
R5310	T8575	T8576	compound	TGF,β
R5311	T8547	T8545	prep	of,buds
R5312	T8576	T8574	compound	β,signaling
R5313	T8577	T8576	punct	-,β
R5314	T8578	T8572	prep	in,display
R5315	T8548	T8549	compound	littermate,skin
R5316	T8549	T8547	pobj	skin,of
R5317	T8579	T8578	pobj	skin,in
R5318	T8580	T8581	punct	[,35
R5319	T8550	T8551	punct	(,6D
R532	T1345	T1343	pobj	remodeling,with
R5320	T8581	T8559	parataxis	35,in
R5321	T8582	T8581	punct	],35
R5322	T8583	T8557	punct	", ",detected
R5323	T8551	T8521	parataxis	6D,found
R5324	T8584	T8585	compound	Snail,protein
R5325	T8585	T8557	nsubjpass	protein,detected
R5326	T8552	T8551	compound	Figure,6D
R5327	T8586	T8557	auxpass	was,detected
R5328	T8587	T8557	advmod	readily,detected
R5329	T8553	T8551	punct	),6D
R533	T1346	T1345	amod	contemporaneous,remodeling
R5330	T8588	T8557	prep	by,detected
R5331	T8554	T8511	punct	.,appeared
R5332	T8589	T8590	compound	Western,blot
R5333	T8590	T8591	compound	blot,analyses
R5334	T8591	T8588	pobj	analyses,by
R5335	T8592	T8557	punct	", ",detected
R5336	T8593	T8594	advmod	where,found
R5337	T8556	T8557	advmod	Conversely,detected
R5338	T8594	T8557	advcl	found,detected
R5339	T8595	T8594	nsubjpass	it,found
R534	T1347	T1345	prep	of,remodeling
R5340	T8596	T8594	auxpass	was,found
R5341	T8597	T8594	neg	not,found
R5342	T8558	T8557	punct	", ",detected
R5343	T8598	T8594	prep	in,found
R5344	T8599	T8600	amod	postnatal,skin
R5345	T8600	T8598	pobj	skin,in
R5346	T8601	T8602	punct	(,6E
R5347	T8664	T8660	advmod	still,formed
R5348	T8602	T8557	parataxis	6E,detected
R5349	T8603	T8602	compound	Figure,6E
R535	T1348	T1349	det	the,properties
R5350	T8604	T8602	punct	),6E
R5351	T8605	T8557	punct	.,detected
R5352	T8607	T8608	advcl	Taken,provide
R5353	T8609	T8607	advmod	together,Taken
R5354	T8610	T8608	punct	", ",provide
R5355	T8611	T8612	det	these,results
R5356	T8666	T8660	prep	in,formed
R5357	T8612	T8608	nsubj	results,provide
R5358	T8613	T8614	amod	compelling,evidence
R5359	T8614	T8608	dobj	evidence,provide
R536	T1349	T1347	pobj	properties,of
R5360	T8615	T8616	mark	that,is
R5361	T8616	T8614	acl	is,evidence
R5362	T8667	T8668	compound	TGF,β2
R5363	T8617	T8618	compound	TGF,β2
R5364	T8618	T8616	nsubj	β2,is
R5365	T8619	T8618	punct	-,β2
R5366	T8668	T8670	npadvmod	β2,null
R5367	T8620	T8621	advmod	functionally,important
R5368	T8621	T8616	acomp	important,is
R5369	T8669	T8668	punct	-,β2
R537	T1350	T1349	amod	physical,properties
R5370	T8622	T8621	prep	for,important
R5371	T8623	T8622	pcomp	inducing,for
R5372	T8624	T8625	compound	Snail,expression
R5373	T8670	T8671	amod	null,skin
R5374	T8625	T8623	dobj	expression,inducing
R5375	T8626	T8625	compound	gene,expression
R5376	T8627	T8623	prep	in,inducing
R5377	T8671	T8666	pobj	skin,in
R5378	T8628	T8629	det	a,manner
R5379	T8629	T8627	pobj	manner,in
R538	T1351	T1350	cc	and,physical
R5380	T8672	T8665	punct	", ",reduced
R5381	T8630	T8631	npadvmod	pSMAD,dependent
R5382	T8631	T8629	amod	dependent,manner
R5383	T8632	T8631	punct	-,dependent
R5384	T8673	T8674	poss	their,number
R5385	T8633	T8623	prep	in,inducing
R5386	T8634	T8635	amod	developing,buds
R5387	T8635	T8633	pobj	buds,in
R5388	T8674	T8665	nsubjpass	number,reduced
R5389	T8636	T8635	compound	hair,buds
R539	T1352	T1350	conj	structural,physical
R5390	T8637	T8608	punct	.,provide
R5391	T8675	T8665	auxpass	was,reduced
R5392	T8639	T8640	mark	Whether,contributes
R5393	T8640	T8644	csubjpass	contributes,addressed
R5394	T8676	T8665	prep	by,reduced
R5395	T8641	T8642	compound	pMARK,activity
R5396	T8642	T8640	nsubj	activity,contributes
R5397	T8643	T8640	advmod	also,contributes
R5398	T8677	T8678	advmod	approximately,50
R5399	T8645	T8640	prep	to,contributes
R54	T206	T190	advcl	dissect,employed
R540	T1353	T1349	prep	of,properties
R5400	T8646	T8647	compound	Snail,induction
R5401	T8647	T8645	pobj	induction,to
R5402	T8648	T8644	auxpass	was,addressed
R5403	T8649	T8644	neg	not,addressed
R5404	T8678	T8679	nummod	50,%
R5405	T8650	T8644	prep	in,addressed
R5406	T8651	T8652	det	the,study
R5407	T8652	T8650	pobj	study,in
R5408	T8679	T8676	pobj	%,by
R5409	T8653	T8652	amod	present,study
R541	T1354	T1355	det	the,cell
R5410	T8654	T8655	punct	[,15
R5411	T8655	T8644	parataxis	15,addressed
R5412	T8680	T8681	punct	[,32
R5413	T8656	T8655	punct	],15
R5414	T8657	T8644	punct	.,addressed
R5415	T8681	T8665	parataxis	32,reduced
R5416	T8659	T8660	mark	Although,formed
R5417	T8660	T8665	advcl	formed,reduced
R5418	T8661	T8662	det	some,buds
R5419	T8682	T8681	punct	],32
R542	T1355	T1353	pobj	cell,of
R5420	T8662	T8660	nsubj	buds,formed
R5421	T8663	T8662	compound	hair,buds
R5422	T8683	T8665	punct	.,reduced
R5423	T8685	T8686	advmod	Thus,appear
R5424	T8687	T8686	punct	", ",appear
R5425	T8770	T8772	compound	β2,signaling
R5426	T8688	T8689	mark	although,impacts
R5427	T8689	T8686	advcl	impacts,appear
R5428	T8771	T8770	punct	-,β2
R5429	T8772	T8766	nsubj	signaling,appeared
R543	T1356	T1325	punct	.,orchestrated
R5430	T8690	T8691	det	the,pathway
R5431	T8773	T8774	aux	to,be
R5432	T8774	T8766	xcomp	be,appeared
R5433	T8775	T8776	det	an,factor
R5434	T8691	T8689	nsubj	pathway,impacts
R5435	T8776	T8774	attr	factor,be
R5436	T8777	T8776	amod	important,factor
R5437	T8692	T8691	acl	mediated,pathway
R5438	T8778	T8776	prep	for,factor
R5439	T8779	T8780	det	the,proliferation
R544	T1358	T1359	prep	Among,offer
R5440	T8693	T8692	prep	by,mediated
R5441	T8780	T8778	pobj	proliferation,for
R5442	T8781	T8780	amod	early,proliferation
R5443	T8782	T8783	dep	that,occurs
R5444	T8694	T8695	compound	TGF,β2
R5445	T8783	T8780	relcl	occurs,proliferation
R5446	T8784	T8783	prep	in,occurs
R5447	T8695	T8697	compound	β2,signaling
R5448	T8785	T8786	det	the,buds
R5449	T8786	T8784	pobj	buds,in
R545	T1360	T1361	det	the,organs
R5450	T8787	T8786	amod	developing,buds
R5451	T8696	T8695	punct	-,β2
R5452	T8788	T8786	compound	hair,buds
R5453	T8789	T8774	punct	", ",be
R5454	T8790	T8791	mark	as,judged
R5455	T8791	T8774	advcl	judged,be
R5456	T8792	T8791	prep	by,judged
R5457	T8793	T8794	amod	anti-Ki67,immunofluorescence
R5458	T8697	T8693	pobj	signaling,by
R5459	T8794	T8792	pobj	immunofluorescence,by
R546	T1361	T1358	pobj	organs,Among
R5460	T8795	T8793	cc	and,anti-Ki67
R5461	T8698	T8699	det	the,step
R5462	T8796	T8793	conj	anti-pMAPK,anti-Ki67
R5463	T8797	T8798	punct	(,6F
R5464	T8798	T8774	parataxis	6F,be
R5465	T8699	T8689	dobj	step,impacts
R5466	T8799	T8798	compound	Figure,6F
R5467	T8800	T8798	cc	and,6F
R5468	T8801	T8798	conj	6G,6F
R5469	T8700	T8699	amod	earliest,step
R547	T1362	T1361	amod	few,organs
R5470	T8802	T8798	punct	),6F
R5471	T8803	T8766	punct	.,appeared
R5472	T8701	T8699	prep	of,step
R5473	T8805	T8806	mark	If,stimulates
R5474	T8806	T8810	advcl	stimulates,expected
R5475	T8702	T8703	amod	epithelial,invagination
R5476	T8807	T8808	compound	TGF,β2
R5477	T8808	T8806	nsubj	β2,stimulates
R5478	T8809	T8808	punct	-,β2
R5479	T8703	T8701	pobj	invagination,of
R548	T1363	T1361	amod	dispensable,organs
R5480	T8811	T8812	compound	Snail,expression
R5481	T8812	T8806	dobj	expression,stimulates
R5482	T8704	T8686	punct	", ",appear
R5483	T8813	T8806	prep	in,stimulates
R5484	T8814	T8815	amod	developing,buds
R5485	T8815	T8813	pobj	buds,in
R5486	T8705	T8686	nsubj	it,appear
R5487	T8816	T8810	punct	", ",expected
R5488	T8817	T8810	nsubjpass	loss,expected
R5489	T8818	T8817	prep	of,loss
R549	T1364	T1359	punct	", ",offer
R5490	T8706	T8686	aux	does,appear
R5491	T8819	T8820	det	this,morphogen
R5492	T8820	T8818	pobj	morphogen,of
R5493	T8707	T8686	neg	not,appear
R5494	T8821	T8810	aux	would,expected
R5495	T8822	T8810	auxpass	be,expected
R5496	T8823	T8824	aux	to,affect
R5497	T8708	T8709	aux	to,be
R5498	T8824	T8810	xcomp	affect,expected
R5499	T8825	T8826	det	the,expression
R55	T207	T208	det	the,contributions
R550	T1365	T1366	compound	hair,follicles
R5500	T8826	T8824	dobj	expression,affect
R5501	T8709	T8686	xcomp	be,appear
R5502	T8827	T8826	prep	of,expression
R5503	T8828	T8827	pobj	genes,of
R5504	T8829	T8830	dep	that,repressed
R5505	T8830	T8828	relcl	repressed,genes
R5506	T8831	T8830	auxpass	are,repressed
R5507	T8832	T8830	advmod	typically,repressed
R5508	T8710	T8709	acomp	essential,be
R5509	T8833	T8830	agent	by,repressed
R551	T1366	T1359	nsubj	follicles,offer
R5510	T8834	T8833	pobj	Snail,by
R5511	T8835	T8810	punct	.,expected
R5512	T8711	T8710	prep	for,essential
R5513	T8712	T8713	compound	bud,morphogenesis
R5514	T8837	T8838	mark	Since,is
R5515	T8713	T8711	pobj	morphogenesis,for
R5516	T8838	T8847	advcl	is,investigated
R5517	T8839	T8840	det	a,target
R5518	T8714	T8686	punct	.,appear
R5519	T8840	T8838	nsubj	target,is
R552	T1367	T1368	det	an,system
R5520	T8841	T8840	amod	major,target
R5521	T8842	T8840	prep	for,target
R5522	T8843	T8844	npadvmod	Snail,mediated
R5523	T8716	T8717	advcl	Consistent,took
R5524	T8844	T8846	amod	mediated,repression
R5525	T8845	T8844	punct	-,mediated
R5526	T8846	T8842	pobj	repression,for
R5527	T8718	T8716	prep	with,Consistent
R5528	T8848	T8849	det	the,gene
R5529	T8849	T8838	attr	gene,is
R553	T1368	T1359	dobj	system,offer
R5530	T8850	T8851	compound	E,cadherin
R5531	T8719	T8720	det	this,notion
R5532	T8720	T8718	pobj	notion,with
R5533	T8721	T8717	punct	", ",took
R5534	T8851	T8849	compound	cadherin,gene
R5535	T8722	T8723	compound	basement,membrane
R5536	T8723	T8724	compound	membrane,remodeling
R5537	T8852	T8851	punct	-,cadherin
R5538	T8853	T8854	punct	[,13
R5539	T8854	T8838	parataxis	13,is
R554	T1369	T1368	amod	excellent,system
R5540	T8724	T8717	nsubj	remodeling,took
R5541	T8855	T8854	nummod	12,13
R5542	T8856	T8854	punct	",",13
R5543	T8725	T8717	advmod	still,took
R5544	T8857	T8854	punct	],13
R5545	T8858	T8847	punct	", ",investigated
R5546	T8859	T8847	nsubj	we,investigated
R5547	T8726	T8717	dobj	place,took
R5548	T8860	T8861	det	the,status
R5549	T8861	T8847	dobj	status,investigated
R555	T1370	T1368	compound	model,system
R5550	T8727	T8717	prep	in,took
R5551	T8862	T8861	prep	of,status
R5552	T8863	T8864	compound	E,cadherin
R5553	T8864	T8862	pobj	cadherin,of
R5554	T8728	T8729	det	the,buds
R5555	T8865	T8864	punct	-,cadherin
R5556	T8866	T8847	prep	in,investigated
R5557	T8867	T8868	compound	TGF,β2
R5558	T8868	T8870	npadvmod	β2,null
R5559	T8869	T8868	punct	-,β2
R556	T1371	T1372	aux	to,study
R5560	T8870	T8872	amod	null,buds
R5561	T8729	T8727	pobj	buds,in
R5562	T8871	T8870	punct	-,null
R5563	T8872	T8866	pobj	buds,in
R5564	T8873	T8847	punct	.,investigated
R5565	T8730	T8731	compound	TGF,β2
R5566	T8875	T8876	mark	As,shown
R5567	T8731	T8733	npadvmod	β2,null
R5568	T8732	T8731	punct	-,β2
R5569	T8733	T8729	amod	null,buds
R557	T1372	T1368	advcl	study,system
R5570	T8734	T8733	punct	-,null
R5571	T8876	T8877	advcl	shown,displayed
R5572	T8735	T8717	punct	", ",took
R5573	T8878	T8876	prep	in,shown
R5574	T8736	T8737	mark	as,judged
R5575	T8879	T8880	compound	Figure,6H
R5576	T8880	T8878	pobj	6H,in
R5577	T8881	T8877	punct	", ",displayed
R5578	T8737	T8717	advcl	judged,took
R5579	T8882	T8883	compound	hair,buds
R558	T1373	T1374	amod	epithelial,bud
R5580	T8883	T8877	nsubj	buds,displayed
R5581	T8884	T8883	prep	in,buds
R5582	T8738	T8737	prep	by,judged
R5583	T8885	T8886	compound	TGF,β2
R5584	T8886	T8888	npadvmod	β2,null
R5585	T8887	T8886	punct	-,β2
R5586	T8739	T8738	pobj	immunofluorescence,by
R5587	T8888	T8889	amod	null,skin
R5588	T8889	T8884	pobj	skin,in
R5589	T8740	T8739	prep	with,immunofluorescence
R559	T1374	T1375	compound	bud,formation
R5590	T8890	T8891	amod	elevated,staining
R5591	T8891	T8877	dobj	staining,displayed
R5592	T8892	T8891	compound	immunofluorescence,staining
R5593	T8741	T8740	pobj	antibodies,with
R5594	T8893	T8877	advcl	relative,displayed
R5595	T8894	T8893	prep	to,relative
R5596	T8895	T8896	poss	their,counterparts
R5597	T8742	T8741	prep	against,antibodies
R5598	T8896	T8894	pobj	counterparts,to
R5599	T8897	T8896	compound	WT,counterparts
R56	T208	T206	dobj	contributions,dissect
R560	T1375	T1372	dobj	formation,study
R5600	T8898	T8877	punct	.,displayed
R5601	T8743	T8744	compound	β4,integrin
R5602	T8900	T8901	advmod	Previously,demonstrated
R5603	T8744	T8742	pobj	integrin,against
R5604	T8902	T8901	nsubj	we,demonstrated
R5605	T8903	T8904	mark	that,required
R5606	T8745	T8744	punct	", ",integrin
R5607	T8904	T8901	ccomp	required,demonstrated
R5608	T8905	T8906	det	the,action
R5609	T8906	T8904	nsubjpass	action,required
R561	T1376	T1359	punct	.,offer
R5610	T8907	T8906	amod	concerted,action
R5611	T8908	T8906	prep	of,action
R5612	T8746	T8747	det	an,component
R5613	T8909	T8910	det	the,signals
R5614	T8910	T8908	pobj	signals,of
R5615	T8911	T8910	amod	extracellular,signals
R5616	T8747	T8744	appos	component,integrin
R5617	T8912	T8910	appos	Wnt,signals
R5618	T8913	T8912	cc	and,Wnt
R5619	T8914	T8912	conj	noggin,Wnt
R562	T1378	T1379	amod	Mammalian,epithelium
R5620	T8748	T8747	amod	integral,component
R5621	T8915	T8904	auxpass	are,required
R5622	T8916	T8917	mark	for,repress
R5623	T8749	T8747	prep	of,component
R5624	T8750	T8751	npadvmod	keratinocyte,mediated
R5625	T8917	T8904	advcl	repress,required
R5626	T8918	T8919	det	the,generation
R5627	T8751	T8753	amod	mediated,adhesion
R5628	T8919	T8917	nsubj	generation,repress
R5629	T8920	T8919	prep	of,generation
R563	T1379	T1381	nsubj	epithelium,begins
R5630	T8921	T8922	det	a,complex
R5631	T8752	T8751	punct	-,mediated
R5632	T8922	T8920	pobj	complex,of
R5633	T8923	T8922	nmod	LEF,complex
R5634	T8924	T8923	punct	-,LEF
R5635	T8753	T8749	pobj	adhesion,of
R5636	T8925	T8923	nummod	1,LEF
R5637	T8926	T8923	punct	/,LEF
R5638	T8927	T8928	compound	β,catenin
R5639	T8754	T8753	prep	to,adhesion
R564	T1380	T1379	compound	skin,epithelium
R5640	T8928	T8923	appos	catenin,LEF
R5641	T8929	T8928	punct	-,catenin
R5642	T8755	T8756	poss	its,membrane
R5643	T8930	T8922	compound	transcription,complex
R5644	T8931	T8917	aux	to,repress
R5645	T8932	T8933	compound	E,cadherin
R5646	T8756	T8754	pobj	membrane,to
R5647	T8933	T8935	compound	cadherin,transcription
R5648	T8934	T8933	punct	-,cadherin
R5649	T8757	T8756	amod	underlying,membrane
R565	T1382	T1381	prep	as,begins
R5650	T8935	T8917	dobj	transcription,repress
R5651	T8936	T8917	prep	at,repress
R5652	T8937	T8938	det	the,onset
R5653	T8758	T8756	compound	basement,membrane
R5654	T8938	T8936	pobj	onset,at
R5655	T8939	T8938	prep	of,onset
R5656	T8759	T8760	punct	(,6F
R5657	T8940	T8941	compound	hair,fate
R5658	T8941	T8942	compound	fate,specification
R5659	T8942	T8939	pobj	specification,of
R566	T1383	T1384	det	a,sheet
R5660	T8943	T8901	punct	.,demonstrated
R5661	T8760	T8717	parataxis	6F,took
R5662	T8945	T8946	mark	As,shown
R5663	T8946	T8947	advcl	shown,exhibited
R5664	T8948	T8946	prep	in,shown
R5665	T8761	T8760	compound	Figure,6F
R5666	T8949	T8950	compound	Figure,6I
R5667	T8950	T8948	pobj	6I,in
R5668	T8762	T8760	punct	),6F
R5669	T8951	T8950	cc	and,6I
R567	T1384	T1382	pobj	sheet,as
R5670	T8952	T8950	conj	6J,6I
R5671	T8953	T8947	punct	", ",exhibited
R5672	T8763	T8717	punct	.,took
R5673	T8954	T8955	preconj	both,WT
R5674	T8955	T8956	npadvmod	WT,null
R5675	T8765	T8766	prep	In,appeared
R5676	T8956	T8961	amod	null,buds
R5677	T8957	T8955	cc	and,WT
R5678	T8958	T8959	compound	TGF,β2
R5679	T8959	T8955	conj	β2,WT
R568	T1385	T1384	amod	single,sheet
R5680	T8960	T8959	punct	-,β2
R5681	T8767	T8765	pobj	contrast,In
R5682	T8961	T8947	nsubj	buds,exhibited
R5683	T8962	T8963	amod	nuclear,localization
R5684	T8963	T8947	dobj	localization,exhibited
R5685	T8964	T8963	nmod	LEF,localization
R5686	T8768	T8766	punct	", ",appeared
R5687	T8965	T8964	punct	-,LEF
R5688	T8966	T8964	nummod	1,LEF
R5689	T8967	T8964	cc	and,LEF
R569	T1386	T1384	prep	of,sheet
R5690	T8769	T8770	compound	TGF,β2
R5691	T8968	T8969	compound	β,catenin
R5692	T8969	T8964	conj	catenin,LEF
R5693	T8970	T8969	punct	-,catenin
R5694	T8982	T8947	punct	.,exhibited
R5695	T8971	T8947	punct	", ",exhibited
R5696	T8972	T8947	npadvmod	signs,exhibited
R5697	T8973	T8974	mark	that,was
R5698	T8974	T8972	acl	was,signs
R5699	T8975	T8976	det	the,pathway
R57	T209	T208	prep	of,contributions
R570	T1387	T1388	amod	multipotent,cells
R5700	T8976	T8974	nsubj	pathway,was
R5701	T8977	T8978	compound	Wnt,noggin
R5702	T8984	T8985	det	These,data
R5703	T8978	T8976	compound	noggin,pathway
R5704	T8979	T8978	punct	-,noggin
R5705	T8980	T8976	compound	signaling,pathway
R5706	T8981	T8974	acomp	intact,was
R5707	T8985	T8986	nsubj	data,suggest
R5708	T8987	T8988	mark	that,functions
R5709	T8988	T8986	ccomp	functions,suggest
R571	T1388	T1386	pobj	cells,of
R5710	T8989	T8988	prep	during,functions
R5711	T8990	T8991	compound	hair,follicle
R5712	T8991	T8992	compound	follicle,morphogenesis
R5713	T8992	T8989	pobj	morphogenesis,during
R5714	T8993	T8988	punct	", ",functions
R5715	T8994	T8995	compound	TGF,β2
R5716	T8995	T8988	nsubj	β2,functions
R5717	T8996	T8995	punct	-,β2
R5718	T8997	T8988	advmod	subsequently,functions
R5719	T8998	T8997	prep	to,subsequently
R572	T1389	T1388	amod	ectodermal,cells
R5720	T8999	T9000	compound	Wnt,noggin
R5721	T9000	T9002	npadvmod	noggin,mediated
R5722	T9001	T9000	punct	/,noggin
R5723	T9002	T9004	amod	mediated,determination
R5724	T9003	T9002	punct	-,mediated
R5725	T9004	T8998	pobj	determination,to
R5726	T9005	T9004	prep	of,determination
R5727	T9006	T9007	compound	hair,fate
R5728	T9007	T9005	pobj	fate,of
R5729	T9008	T8986	punct	.,suggest
R573	T1390	T1381	punct	.,begins
R5730	T9010	T9011	advmod	Moreover,appears
R5731	T9012	T9011	punct	", ",appears
R5732	T9013	T9011	prep	through,appears
R5733	T9014	T9013	pobj	activation,through
R5734	T9015	T9014	prep	of,activation
R5735	T9016	T9017	compound	Snail,expression
R5736	T9017	T9015	pobj	expression,of
R5737	T9018	T9017	compound	gene,expression
R5738	T9019	T9011	punct	", ",appears
R5739	T9020	T9021	compound	TGF,β2
R574	T1392	T1393	prep	During,populate
R5740	T9021	T9011	nsubj	β2,appears
R5741	T9022	T9021	punct	-,β2
R5742	T9023	T9024	aux	to,work
R5743	T9024	T9011	xcomp	work,appears
R5744	T9025	T9024	prep	in,work
R5745	T9026	T9025	pobj	tandem,in
R5746	T9027	T9026	prep	with,tandem
R5747	T9028	T9029	det	these,morphogens
R5748	T9029	T9027	pobj	morphogens,with
R5749	T9030	T9029	amod	other,morphogens
R575	T1394	T1392	pobj	development,During
R5750	T9031	T9032	aux	to,regulate
R5751	T9032	T9024	advcl	regulate,work
R5752	T9033	T9032	advmod	down,regulate
R5753	T9034	T9032	punct	-,regulate
R5754	T9035	T9036	compound	E,cadherin
R5755	T9036	T9038	compound	cadherin,levels
R5756	T9037	T9036	punct	-,cadherin
R5757	T9038	T9032	dobj	levels,regulate
R5758	T9039	T9024	punct	", ",work
R5759	T9040	T9041	dep	which,contributes
R576	T1395	T1393	punct	", ",populate
R5760	T9041	T9024	advcl	contributes,work
R5761	T9042	T9041	prep	to,contributes
R5762	T9043	T9044	det	the,activation
R5763	T9044	T9042	pobj	activation,to
R5764	T9045	T9044	prep	of,activation
R5765	T9046	T9047	amod	proliferative,circuitries
R5766	T9047	T9045	pobj	circuitries,of
R5767	T9048	T9011	punct	.,appears
R577	T1396	T1397	amod	specialized,cells
R5772	T9102	T9103	prep	During,govern
R5773	T9104	T9105	compound	budding,morphogenesis
R5774	T9105	T9102	pobj	morphogenesis,During
R5775	T9106	T9103	punct	", ",govern
R5776	T9107	T9108	amod	intersecting,networks
R5777	T9108	T9103	nsubj	networks,govern
R5778	T9109	T9108	compound	signaling,networks
R5779	T9110	T9108	prep	from,networks
R578	T1397	T1393	nsubj	cells,populate
R5780	T9111	T9112	det	the,epithelium
R5781	T9112	T9110	pobj	epithelium,from
R5782	T9113	T9112	cc	and,epithelium
R5783	T9114	T9112	conj	mesenchyme,epithelium
R5784	T9115	T9116	amod	transcriptional,programs
R5785	T9116	T9103	dobj	programs,govern
R5786	T9117	T9115	punct	", ",transcriptional
R5787	T9118	T9115	conj	adhesive,transcriptional
R5788	T9119	T9118	punct	", ",adhesive
R5789	T9120	T9118	conj	polarity,adhesive
R579	T1398	T1397	amod	mesenchymal,cells
R5790	T9121	T9120	punct	", ",polarity
R5791	T9122	T9120	cc	and,polarity
R5792	T9123	T9120	conj	motility,polarity
R5793	T9124	T9103	prep	in,govern
R5794	T9125	T9126	det	these,groups
R5795	T9126	T9124	pobj	groups,in
R5796	T9127	T9126	amod	select,groups
R5797	T9128	T9126	prep	of,groups
R5798	T9129	T9128	pobj	cells,of
R5799	T9130	T9103	punct	.,govern
R58	T210	T211	amod	multiple,cues
R580	T1399	T1400	det	the,skin
R5800	T9132	T9133	det	The,changes
R5801	T9133	T9138	nsubj	changes,form
R5802	T9134	T9133	amod	dynamic,changes
R5803	T9135	T9133	amod	nuclear,changes
R5804	T9136	T9135	cc	and,nuclear
R5805	T9137	T9135	conj	cytosolic,nuclear
R5806	T9139	T9140	dep	that,take
R5807	T9140	T9133	relcl	take,changes
R5808	T9141	T9140	dobj	place,take
R5809	T9142	T9140	prep	during,take
R581	T1400	T1393	dobj	skin,populate
R5810	T9143	T9144	det	this,time
R5811	T9144	T9142	pobj	time,during
R5812	T9145	T9146	det	the,cornerstone
R5813	T9146	T9138	dobj	cornerstone,form
R5814	T9147	T9146	prep	for,cornerstone
R5815	T9148	T9149	compound	organ,morphogenesis
R5816	T9149	T9147	pobj	morphogenesis,for
R5817	T9150	T9138	punct	.,form
R5818	T9152	T9153	nummod	Two,challenges
R5819	T9153	T9155	nsubj	challenges,are
R582	T1401	T1393	prep	in,populate
R5820	T9154	T9153	amod	major,challenges
R5821	T9156	T9153	prep	in,challenges
R5822	T9157	T9156	pcomp	understanding,in
R5823	T9158	T9159	det	the,mechanisms
R5824	T9159	T9157	dobj	mechanisms,understanding
R5825	T9160	T9159	acl	underlying,mechanisms
R5826	T9161	T9162	det	a,process
R5827	T9162	T9160	dobj	process,underlying
R5828	T9163	T9162	amod	particular,process
R5829	T9164	T9162	compound	budding,process
R583	T1402	T1403	det	a,pattern
R5830	T9165	T9166	aux	to,order
R5831	T9166	T9155	xcomp	order,are
R5832	T9167	T9168	det	the,sequence
R5833	T9168	T9166	dobj	sequence,order
R5834	T9169	T9168	amod	temporal,sequence
R5835	T9170	T9168	prep	of,sequence
R5836	T9171	T9172	amod	external,cues
R5837	T9172	T9170	pobj	cues,of
R5838	T9173	T9172	acl	involved,cues
R5839	T9174	T9166	cc	and,order
R584	T1403	T1401	pobj	pattern,in
R5840	T9175	T9176	advmod	then,dissect
R5841	T9176	T9166	conj	dissect,order
R5842	T9177	T9176	aux	to,dissect
R5843	T9178	T9179	advmod	how,translate
R5844	T9179	T9176	ccomp	translate,dissect
R5845	T9180	T9181	det	the,cells
R5846	T9181	T9179	nsubj	cells,translate
R5847	T9182	T9181	prep	of,cells
R5848	T9183	T9184	det	the,bud
R5849	T9184	T9182	pobj	bud,of
R585	T1404	T1405	advmod	spatially,defined
R5850	T9185	T9184	amod	developing,bud
R5851	T9186	T9187	det	these,signals
R5852	T9187	T9179	dobj	signals,translate
R5853	T9188	T9179	prep	into,translate
R5854	T9189	T9190	det	the,events
R5855	T9190	T9188	pobj	events,into
R5856	T9191	T9190	amod	downstream,events
R5857	T9192	T9190	prep	of,events
R5858	T9193	T9194	amod	cellular,remodeling
R5859	T9194	T9192	pobj	remodeling,of
R586	T1405	T1403	amod	defined,pattern
R5860	T9195	T9194	punct	", ",remodeling
R5861	T9196	T9194	conj	proliferation,remodeling
R5862	T9197	T9196	punct	", ",proliferation
R5863	T9198	T9196	cc	and,proliferation
R5864	T9199	T9196	conj	differentiation,proliferation
R5865	T9200	T9155	punct	.,are
R5866	T9202	T9203	poss	Our,studies
R5867	T9203	T9204	nsubj	studies,provide
R5868	T9205	T9203	advmod	here,studies
R5869	T9206	T9207	det	some,insights
R587	T1406	T1407	aux	to,initiate
R5870	T9207	T9204	dobj	insights,provide
R5871	T9208	T9207	prep	into,insights
R5872	T9209	T9210	advmod	how,orchestrated
R5873	T9210	T9208	pcomp	orchestrated,into
R5874	T9211	T9212	det	these,events
R5875	T9212	T9210	nsubjpass	events,orchestrated
R5876	T9213	T9210	auxpass	are,orchestrated
R5877	T9214	T9210	prep	during,orchestrated
R5878	T9215	T9216	compound	hair,bud
R5879	T9216	T9217	compound	bud,formation
R588	T1407	T1393	advcl	initiate,populate
R5880	T9217	T9214	pobj	formation,during
R5881	T9218	T9210	prep	in,orchestrated
R5882	T9219	T9220	amod	developing,skin
R5883	T9220	T9218	pobj	skin,in
R5884	T9221	T9204	punct	.,provide
R589	T1408	T1409	det	the,crosstalk
R5895	T9654	T9653	prep	during,Signaling
R5896	T9655	T9656	amod	Early,Morphogenesis
R5897	T9656	T9654	pobj	Morphogenesis,during
R5898	T9657	T9658	compound	Hair,Follicle
R5899	T9658	T9656	compound	Follicle,Morphogenesis
R59	T211	T209	pobj	cues,of
R590	T1409	T1407	dobj	crosstalk,initiate
R5900	T9660	T9661	amod	Recent,studies
R5901	T9661	T9662	nsubj	studies,suggest
R5902	T9663	T9661	prep	on,studies
R5903	T9664	T9665	compound	hair,bud
R5904	T9665	T9666	compound	bud,morphogenesis
R5905	T9666	T9663	pobj	morphogenesis,on
R5906	T9667	T9668	mark	that,converge
R5907	T9668	T9662	ccomp	converge,suggest
R5908	T9669	T9670	compound	Wnt,signals
R5909	T9670	T9668	nsubj	signals,converge
R591	T1410	T1409	amod	complex,crosstalk
R5910	T9671	T9672	advmod	likely,from
R5911	T9672	T9670	prep	from,signals
R5912	T9673	T9674	det	the,epithelium
R5913	T9674	T9672	pobj	epithelium,from
R5914	T9675	T9670	cc	and,signals
R5915	T9676	T9677	nmod	BMP,signals
R5916	T9677	T9670	conj	signals,signals
R5917	T9678	T9677	amod	inhibitory,signals
R5918	T9679	T9677	prep	from,signals
R5919	T9680	T9681	det	the,mesenchyme
R592	T1411	T1412	amod	epithelial,mesenchymal
R5920	T9681	T9679	pobj	mesenchyme,from
R5921	T9682	T9681	amod	underlying,mesenchyme
R5922	T9683	T9684	aux	to,produce
R5923	T9684	T9668	advcl	produce,converge
R5924	T9685	T9686	det	an,complex
R5925	T9686	T9684	dobj	complex,produce
R5926	T9687	T9686	amod	active,complex
R5927	T9688	T9689	compound	transcription,factor
R5928	T9689	T9686	compound	factor,complex
R5929	T9690	T9686	acl	involving,complex
R593	T1412	T1409	amod	mesenchymal,crosstalk
R5930	T9691	T9692	compound	β,catenin
R5931	T9692	T9690	dobj	catenin,involving
R5932	T9693	T9692	punct	-,catenin
R5933	T9694	T9692	cc	and,catenin
R5934	T9695	T9692	conj	LEF,catenin
R5935	T9696	T9695	punct	-,LEF
R5936	T9697	T9695	nummod	1,LEF
R5937	T9698	T9686	punct	", ",complex
R5938	T9699	T9700	dep	which,plays
R5939	T9700	T9686	relcl	plays,complex
R594	T1413	T1412	punct	-,mesenchymal
R5940	T9701	T9700	prep	in,plays
R5941	T9702	T9701	pobj	turn,in
R5942	T9703	T9704	det	a,role
R5943	T9704	T9700	dobj	role,plays
R5944	T9705	T9704	amod	key,role
R5945	T9706	T9700	prep	in,plays
R5946	T9707	T9706	pcomp	specifying,in
R5947	T9708	T9709	det	the,fate
R5948	T9709	T9707	dobj	fate,specifying
R5949	T9710	T9711	compound	hair,follicle
R595	T1414	T1415	dep	that,specify
R5950	T9711	T9709	compound	follicle,fate
R5951	T9712	T9713	punct	[,37
R5952	T9713	T9684	parataxis	37,produce
R5953	T9714	T9713	nummod	4,37
R5954	T9715	T9713	punct	",",37
R5955	T9716	T9713	nummod	29,37
R5956	T9717	T9713	punct	",",37
R5957	T9718	T9713	nummod	30,37
R5958	T9719	T9713	punct	",",37
R5959	T9720	T9713	nummod	36,37
R596	T1415	T1409	relcl	specify,crosstalk
R5960	T9721	T9713	punct	",",37
R5961	T9722	T9713	punct	],37
R5962	T9723	T9662	punct	.,suggest
R5963	T9725	T9726	amod	Sonic,hedgehog
R5964	T9726	T9727	nsubj	hedgehog,play
R5965	T9728	T9726	punct	(,hedgehog
R5966	T9729	T9726	appos	Shh,hedgehog
R5967	T9730	T9726	punct	),hedgehog
R5968	T9731	T9726	cc	and,hedgehog
R5969	T9732	T9733	compound	TGF,β2
R597	T1416	T1415	aux	will,specify
R5970	T9733	T9735	compound	β2,signaling
R5971	T9734	T9733	punct	-,β2
R5972	T9735	T9726	conj	signaling,hedgehog
R5973	T9736	T9727	advmod	also,play
R5974	T9737	T9738	amod	essential,roles
R5975	T9738	T9727	dobj	roles,play
R5976	T9739	T9727	prep	in,play
R5977	T9740	T9741	compound	follicle,morphogenesis
R5978	T9741	T9739	pobj	morphogenesis,in
R5979	T9742	T9727	punct	", ",play
R598	T1417	T1418	det	the,bud
R5980	T9743	T9727	cc	but,play
R5981	T9744	T9745	prep	in,form
R5982	T9745	T9727	conj	form,play
R5983	T9746	T9744	pobj	contrast,in
R5984	T9747	T9746	prep	to,contrast
R5985	T9748	T9749	compound	β,catenin
R5986	T9749	T9751	npadvmod	catenin,null
R5987	T9750	T9749	punct	-,catenin
R5988	T9751	T9752	amod	null,skin
R5989	T9752	T9747	pobj	skin,to
R599	T1418	T1415	dobj	bud,specify
R5990	T9753	T9752	punct	", ",skin
R5991	T9754	T9755	prep	in,are
R5992	T9755	T9752	relcl	are,skin
R5993	T9756	T9754	pobj	which,in
R5994	T9757	T9758	compound	follicle,invaginations
R5995	T9758	T9755	nsubj	invaginations,are
R5996	T9759	T9755	acomp	absent,are
R5997	T9760	T9761	punct	[,30
R5998	T9761	T9747	parataxis	30,to
R5999	T9762	T9761	punct	],30
R6	T153	T152	punct	-,β2
R60	T212	T211	amod	extracellular,cues
R600	T1419	T1420	punct	[,3
R6000	T9763	T9745	punct	", ",form
R6001	T9764	T9765	det	some,buds
R6002	T9765	T9745	nsubj	buds,form
R6003	T9766	T9765	compound	hair,buds
R6004	T9767	T9745	advmod	still,form
R6005	T9768	T9745	prep	in,form
R6006	T9769	T9770	det	the,absence
R6007	T9770	T9768	pobj	absence,in
R6008	T9771	T9770	prep	of,absence
R6009	T9772	T9771	pobj	LEF,of
R601	T1420	T1393	parataxis	3,populate
R6010	T9773	T9772	punct	-,LEF
R6011	T9774	T9772	nummod	1,LEF
R6012	T9775	T9772	punct	", ",LEF
R6013	T9776	T9772	conj	Shh,LEF
R6014	T9777	T9776	punct	", ",Shh
R6015	T9778	T9776	cc	or,Shh
R6016	T9779	T9780	compound	TGF,β2
R6017	T9780	T9776	conj	β2,Shh
R6018	T9781	T9780	punct	-,β2
R6019	T9782	T9783	punct	[,38
R602	T1421	T1420	punct	],3
R6020	T9783	T9745	parataxis	38,form
R6021	T9784	T9783	nummod	32,38
R6022	T9785	T9783	punct	",",38
R6023	T9786	T9783	punct	],38
R6024	T9787	T9745	punct	.,form
R6025	T9789	T9790	nsubj	These,reflect
R6026	T9791	T9790	advmod	likely,reflect
R6027	T9792	T9793	det	the,wave
R6028	T9793	T9790	dobj	wave,reflect
R6029	T9794	T9793	amod	first,wave
R603	T1422	T1393	punct	.,populate
R6030	T9795	T9793	prep	of,wave
R6031	T9796	T9797	nmod	follicle,morphogenesis
R6032	T9797	T9795	pobj	morphogenesis,of
R6033	T9798	T9799	punct	(,hair
R6034	T9799	T9797	parataxis	hair,morphogenesis
R6035	T9800	T9799	advmod	i.e.,hair
R6036	T9801	T9799	punct	", ",hair
R6037	T9802	T9799	compound	guard,hair
R6038	T9803	T9799	punct	),hair
R6039	T9804	T9793	punct	", ",wave
R604	T1424	T1425	mark	Once,committed
R6040	T9805	T9806	dep	which,accounts
R6041	T9806	T9793	relcl	accounts,wave
R6042	T9807	T9806	prep	for,accounts
R6043	T9808	T9809	det	a,number
R6044	T9809	T9807	pobj	number,for
R6045	T9810	T9809	amod	small,number
R6046	T9811	T9809	punct	(,number
R6047	T9812	T9813	amod	fewer,5
R6048	T9813	T9815	nummod	5,%
R6049	T9814	T9813	quantmod	than,5
R605	T1425	T1426	advcl	committed,instructs
R6050	T9815	T9809	appos	%,number
R6051	T9816	T9809	punct	),number
R6052	T9817	T9809	prep	of,number
R6053	T9818	T9817	pobj	hairs,of
R6054	T9819	T9806	cc	and,accounts
R6055	T9820	T9806	conj	is,accounts
R6056	T9821	T9820	prep	under,is
R6057	T9822	T9823	amod	distinct,control
R6058	T9823	T9821	pobj	control,under
R6059	T9824	T9823	amod	regulatory,control
R606	T1427	T1426	punct	", ",instructs
R6060	T9825	T9790	punct	.,reflect
R6061	T9827	T9828	compound	Guard,hairs
R6062	T9828	T9829	nsubj	hairs,form
R6063	T9830	T9829	prep	in,form
R6064	T9831	T9832	det	the,absence
R6065	T9832	T9830	pobj	absence,in
R6066	T9833	T9832	prep	of,absence
R6067	T9834	T9833	pobj	LEF,of
R6068	T9835	T9834	punct	-,LEF
R6069	T9836	T9834	nummod	1,LEF
R607	T1428	T1429	det	a,cluster
R6070	T9837	T9834	cc	and,LEF
R6071	T9838	T9839	compound	TGF,β2
R6072	T9839	T9834	conj	β2,LEF
R6073	T9840	T9839	punct	-,β2
R6074	T9841	T9829	punct	", ",form
R6075	T9842	T9829	cc	and,form
R6076	T9843	T9844	nsubj	we,found
R6077	T9844	T9829	conj	found,form
R6078	T9845	T9844	aux	have,found
R6079	T9846	T9847	mark	that,fail
R608	T1429	T1426	nsubj	cluster,instructs
R6080	T9847	T9844	ccomp	fail,found
R6081	T9848	T9847	nsubj	they,fail
R6082	T9849	T9847	advmod	also,fail
R6083	T9850	T9851	aux	to,express
R6084	T9851	T9847	xcomp	express,fail
R6085	T9852	T9851	dobj	Snail,express
R6086	T9853	T9847	prep	at,fail
R6087	T9854	T9855	det	the,stage
R6088	T9855	T9853	pobj	stage,at
R6089	T9856	T9855	compound	budding,stage
R609	T1430	T1429	amod	small,cluster
R6090	T9857	T9855	prep	of,stage
R6091	T9858	T9857	pobj	development,of
R6092	T9859	T9860	punct	(,data
R6093	T9860	T9844	meta	data,found
R6094	T9861	T9860	amod	unpublished,data
R6095	T9862	T9860	punct	),data
R6096	T9863	T9844	punct	.,found
R6097	T9865	T9866	advmod	How,regulated
R6098	T9866	T9871	csubj	regulated,remains
R6099	T9867	T9868	compound	E,cadherin
R61	T213	T208	prep	in,contributions
R610	T1431	T1429	prep	of,cluster
R6100	T9868	T9866	nsubjpass	cadherin,regulated
R6101	T9869	T9868	punct	-,cadherin
R6102	T9870	T9866	auxpass	is,regulated
R6103	T9872	T9866	prep	in,regulated
R6104	T9873	T9874	compound	guard,hairs
R6105	T9874	T9872	pobj	hairs,in
R6106	T9875	T9876	aux	to,determined
R6107	T9876	T9871	xcomp	determined,remains
R6108	T9877	T9876	auxpass	be,determined
R6109	T9878	T9871	punct	.,remains
R611	T1432	T1433	amod	epithelial,cells
R6110	T9880	T9881	amod	Several,candidates
R6111	T9881	T9882	nsubj	candidates,include
R6112	T9883	T9884	amod	other,members
R6113	T9884	T9882	dobj	members,include
R6114	T9885	T9884	compound	Snail,members
R6115	T9886	T9884	compound	family,members
R6116	T9887	T9888	amod	such,as
R6117	T9888	T9884	prep	as,members
R6118	T9889	T9888	pobj	Slug,as
R6119	T9890	T9889	cc	or,Slug
R612	T1433	T1431	pobj	cells,of
R6120	T9891	T9889	conj	twist,Slug
R6121	T9892	T9884	punct	", ",members
R6122	T9893	T9884	cc	or,members
R6123	T9894	T9895	advmod	alternatively,factors
R6124	T9895	T9884	conj	factors,members
R6125	T9896	T9895	punct	", ",factors
R6126	T9897	T9895	compound	transcription,factors
R6127	T9898	T9895	acl	involving,factors
R6128	T9899	T9900	compound	β,catenin
R6129	T9900	T9898	dobj	catenin,involving
R613	T1434	T1429	punct	", ",cluster
R6130	T9901	T9900	punct	-,catenin
R6131	T9902	T9900	cc	and,catenin
R6132	T9903	T9904	det	a,member
R6133	T9904	T9900	conj	member,catenin
R6134	T9905	T9904	amod	different,member
R6135	T9906	T9904	prep	of,member
R6136	T9907	T9908	det	the,family
R6137	T9908	T9906	pobj	family,of
R6138	T9909	T9910	nmod	LEF,box
R6139	T9910	T9908	nmod	box,family
R614	T1435	T1436	det	the,placode
R6140	T9911	T9909	punct	-,LEF
R6141	T9912	T9909	nummod	1,LEF
R6142	T9913	T9909	punct	/,LEF
R6143	T9914	T9909	appos	TCF,LEF
R6144	T9915	T9909	punct	/,LEF
R6145	T9916	T9917	compound	Sry,type
R6146	T9917	T9909	appos	type,LEF
R6147	T9918	T9917	punct	-,type
R6148	T9919	T9910	nmod	HMG,box
R6149	T9920	T9921	punct	(,known
R615	T1436	T1429	appos	placode,cluster
R6150	T9921	T9910	parataxis	known,box
R6151	T9922	T9921	advmod	commonly,known
R6152	T9923	T9921	prep	as,known
R6153	T9924	T9923	pobj	SOX,as
R6154	T9925	T9921	punct	),known
R6155	T9926	T9927	punct	[,40
R6156	T9927	T9882	parataxis	40,include
R6157	T9928	T9927	nummod	39,40
R6158	T9929	T9927	punct	",",40
R6159	T9930	T9927	punct	],40
R616	T1437	T1426	punct	", ",instructs
R6160	T9931	T9882	punct	.,include
R6161	T9933	T9934	amod	Further,investigation
R6162	T9934	T9935	nsubjpass	investigation,required
R6163	T9936	T9935	aux	will,required
R6164	T9937	T9935	auxpass	be,required
R6165	T9938	T9939	aux	to,determine
R6166	T9939	T9935	advcl	determine,required
R6167	T9940	T9941	mark	whether,is
R6168	T9941	T9939	ccomp	is,determine
R6169	T9942	T9943	det	the,pathway
R617	T1438	T1439	det	a,group
R6170	T9943	T9941	nsubj	pathway,is
R6171	T9944	T9943	compound	signaling,pathway
R6172	T9945	T9946	nsubj	we,elucidated
R6173	T9946	T9943	advcl	elucidated,pathway
R6174	T9947	T9946	aux	have,elucidated
R6175	T9948	T9946	advmod	here,elucidated
R6176	T9949	T9950	det	a,theme
R6177	T9950	T9941	attr	theme,is
R6178	T9951	T9950	prep	with,theme
R6179	T9952	T9953	amod	multiple,variations
R618	T1439	T1440	nsubj	group,condense
R6180	T9953	T9951	pobj	variations,with
R6181	T9954	T9935	punct	.,required
R6182	T9956	T9957	compound	TGF,βs
R6183	T9957	T9959	nsubjpass	βs,known
R6184	T9958	T9957	punct	-,βs
R6185	T9960	T9959	auxpass	are,known
R6186	T9961	T9962	aux	to,promote
R6187	T9962	T9959	xcomp	promote,known
R6188	T9963	T9962	dobj	withdrawal,promote
R6189	T9964	T9963	prep	of,withdrawal
R619	T1440	T1426	ccomp	condense,instructs
R6190	T9965	T9964	pobj	keratinocytes,of
R6191	T9966	T9963	prep	from,withdrawal
R6192	T9967	T9968	det	the,cycle
R6193	T9968	T9966	pobj	cycle,from
R6194	T9969	T9968	compound	cell,cycle
R6195	T9970	T9971	punct	[,41
R6196	T9971	T9959	parataxis	41,known
R6197	T9972	T9971	punct	],41
R6198	T9973	T9959	punct	.,known
R6199	T9975	T9976	advmod	Hence,focused
R62	T214	T213	pcomp	orchestrating,in
R620	T1441	T1439	prep	of,group
R6200	T9977	T9976	punct	", ",focused
R6201	T9978	T9979	advmod	when,detected
R6202	T9979	T9976	advcl	detected,focused
R6203	T9980	T9981	compound	TGF,β2
R6204	T9981	T9983	compound	β2,protein
R6205	T9982	T9981	punct	-,β2
R6206	T9983	T9979	nsubjpass	protein,detected
R6207	T9984	T9979	auxpass	was,detected
R6208	T9985	T9979	prep	at,detected
R6209	T9986	T9987	det	the,transition
R621	T1442	T1443	amod	underlying,cells
R6210	T9987	T9985	pobj	transition,at
R6211	T9988	T9987	prep	between,transition
R6212	T9989	T9990	det	the,phases
R6213	T9990	T9988	pobj	phases,between
R6214	T9991	T9992	npadvmod	growing,destructive
R6215	T9992	T9990	amod	destructive,phases
R6216	T9993	T9992	cc	and,destructive
R6217	T9994	T9990	prep	of,phases
R6218	T9995	T9996	det	the,cycle
R6219	T9996	T9994	pobj	cycle,of
R622	T1443	T1441	pobj	cells,of
R6220	T9997	T9996	amod	adult,cycle
R6221	T9998	T9996	compound	hair,cycle
R6222	T9999	T9976	punct	", ",focused
R6223	T10000	T9976	nsubj	research,focused
R6224	T10001	T9976	advmod	initially,focused
R6225	T10002	T10001	cc	and,initially
R6226	T10003	T10001	conj	naturally,initially
R6227	T10004	T9976	prep	on,focused
R6228	T10005	T10006	det	a,role
R6229	T10006	T10004	pobj	role,on
R623	T1444	T1443	amod	mesenchymal,cells
R6230	T10007	T10006	prep	for,role
R6231	T10008	T10009	det	this,member
R6232	T10009	T10007	pobj	member,for
R6233	T10010	T10009	compound	family,member
R6234	T10011	T10006	prep	in,role
R6235	T10012	T10011	pobj	cessation,in
R6236	T10013	T10012	prep	of,cessation
R6237	T10014	T10013	pobj	growth,of
R6238	T10015	T10012	cc	and,cessation
R6239	T10016	T10015	punct	/,and
R624	T1445	T1440	aux	to,condense
R6240	T10017	T10015	cc	or,and
R6241	T10018	T10012	conj	triggering,cessation
R6242	T10019	T10018	dobj	apoptosis,triggering
R6243	T10020	T10021	punct	(,42
R6244	T10021	T9976	parataxis	42,focused
R6245	T10022	T10021	punct	[,42
R6246	T10023	T10021	punct	],42
R6247	T10024	T10021	cc	and,42
R6248	T10025	T10021	conj	references,42
R6249	T10026	T10025	advmod	therein,references
R625	T1446	T1440	cc	and,condense
R6250	T10027	T10021	punct	),42
R6251	T10028	T9976	punct	.,focused
R6252	T10030	T10031	advmod	However,displays
R6253	T10032	T10031	punct	", ",displays
R6254	T10033	T10031	prep	in,displays
R6255	T10034	T10033	pobj	contrast,in
R6256	T10035	T10034	prep	to,contrast
R6257	T10036	T10037	compound	TGF,β1
R6258	T10037	T10039	npadvmod	β1,null
R6259	T10038	T10037	punct	-,β1
R626	T1447	T1440	conj	form,condense
R6260	T10039	T10041	amod	null,skin
R6261	T10040	T10039	punct	-,null
R6262	T10041	T10035	pobj	skin,to
R6263	T10042	T10041	punct	", ",skin
R6264	T10043	T10044	dep	which,exhibits
R6265	T10044	T10041	relcl	exhibits,skin
R6266	T10045	T10046	det	an,phase
R6267	T10046	T10044	dobj	phase,exhibits
R6268	T10047	T10046	amod	extended,phase
R6269	T10048	T10046	compound	growing,phase
R627	T1448	T1449	det	the,papilla
R6270	T10049	T10046	prep	of,phase
R6271	T10050	T10051	amod	postnatal,follicles
R6272	T10051	T10049	pobj	follicles,of
R6273	T10052	T10051	compound	hair,follicles
R6274	T10053	T10031	punct	", ",displays
R6275	T10054	T10055	compound	TGF,β2
R6276	T10055	T10057	npadvmod	β2,null
R6277	T10056	T10055	punct	-,β2
R6278	T10057	T10059	amod	null,skin
R6279	T10058	T10057	punct	-,null
R628	T1449	T1447	dobj	papilla,form
R6280	T10059	T10031	nsubj	skin,displays
R6281	T10060	T10061	det	an,block
R6282	T10061	T10031	dobj	block,displays
R6283	T10062	T10061	amod	embryonic,block
R6284	T10063	T10061	prep	in,block
R6285	T10064	T10065	compound	follicle,bud
R6286	T10065	T10066	compound	bud,progression
R6287	T10066	T10063	pobj	progression,in
R6288	T10067	T10068	punct	[,32
R6289	T10068	T10031	parataxis	32,displays
R629	T1450	T1449	amod	nascent,papilla
R6290	T10069	T10068	punct	],32
R6291	T10070	T10031	punct	.,displays
R6292	T10072	T10073	mark	Although,is
R6293	T10073	T10076	advcl	is,appear
R6294	T10074	T10075	det	this,phenotype
R6295	T10075	T10073	nsubj	phenotype,is
R6296	T10077	T10073	acomp	consistent,is
R6297	T10078	T10077	prep	with,consistent
R6298	T10079	T10080	compound	TGF,β2
R6299	T10080	T10082	poss	β2,patterns
R63	T215	T216	compound	adhesion,dynamics
R630	T1451	T1449	amod	dermal,papilla
R6300	T10081	T10080	punct	-,β2
R6301	T10082	T10078	pobj	patterns,with
R6302	T10083	T10080	case	's,β2
R6303	T10084	T10082	amod	embryonic,patterns
R6304	T10085	T10082	compound	expression,patterns
R6305	T10086	T10087	punct	[,33
R6306	T10087	T10073	parataxis	33,is
R6307	T10088	T10087	punct	],33
R6308	T10089	T10076	punct	", ",appear
R6309	T10090	T10091	quantmod	about,50
R631	T1452	T1449	punct	", ",papilla
R6310	T10091	T10092	nummod	50,%
R6311	T10092	T10076	nsubj	%,appear
R6312	T10093	T10092	prep	of,%
R6313	T10094	T10095	compound	TGF,β2
R6314	T10095	T10097	npadvmod	β2,null
R6315	T10096	T10095	punct	-,β2
R6316	T10097	T10098	amod	null,buds
R6317	T10098	T10093	pobj	buds,of
R6318	T10099	T10076	oprd	unable,appear
R6319	T10100	T10101	aux	to,progress
R632	T1453	T1454	dep	which,be
R6320	T10101	T10099	xcomp	progress,unable
R6321	T10102	T10101	prep	to,progress
R6322	T10103	T10104	det	the,phase
R6323	T10104	T10102	pobj	phase,to
R6324	T10105	T10106	amod	down,growth
R6325	T10106	T10104	compound	growth,phase
R6326	T10107	T10106	punct	-,growth
R6327	T10108	T10099	punct	", ",unable
R6328	T10109	T10110	det	a,feature
R6329	T10110	T10099	npadvmod	feature,unable
R633	T1454	T1449	relcl	be,papilla
R6330	T10111	T10112	dep	that,explained
R6331	T10112	T10110	relcl	explained,feature
R6332	T10113	T10112	aux	can,explained
R6333	T10114	T10112	neg	not,explained
R6334	T10115	T10112	auxpass	be,explained
R6335	T10116	T10112	advmod	readily,explained
R6336	T10117	T10112	prep	on,explained
R6337	T10118	T10119	det	the,basis
R6338	T10119	T10117	pobj	basis,on
R6339	T10120	T10119	prep	of,basis
R634	T1455	T1454	aux	will,be
R6340	T10121	T10122	advmod	previously,established
R6341	T10122	T10123	amod	established,effects
R6342	T10123	T10120	pobj	effects,of
R6343	T10124	T10123	prep	of,effects
R6344	T10125	T10126	compound	TGF,βs
R6345	T10126	T10124	pobj	βs,of
R6346	T10127	T10126	punct	-,βs
R6347	T10128	T10076	punct	.,appear
R6348	T10130	T10131	poss	Our,finding
R6349	T10131	T10132	nsubj	finding,lends
R635	T1456	T1457	det	a,fixture
R6350	T10133	T10134	mark	that,is
R6351	T10134	T10131	acl	is,finding
R6352	T10135	T10136	compound	TGF,β2
R6353	T10136	T10134	nsubj	β2,is
R6354	T10137	T10136	punct	-,β2
R6355	T10138	T10134	advmod	upstream,is
R6356	T10139	T10138	prep	from,upstream
R6357	T10140	T10141	compound	Ki67,expression
R6358	T10141	T10139	pobj	expression,from
R6359	T10142	T10141	cc	and,expression
R636	T1457	T1454	attr	fixture,be
R6360	T10143	T10144	compound	MAPK,activation
R6361	T10144	T10141	conj	activation,expression
R6362	T10145	T10146	amod	further,support
R6363	T10146	T10132	dobj	support,lends
R6364	T10147	T10132	dative	to,lends
R6365	T10148	T10149	det	the,notion
R6366	T10149	T10147	pobj	notion,to
R6367	T10150	T10151	mark	that,react
R6368	T10151	T10149	acl	react,notion
R6369	T10152	T10153	compound	hair,follicle
R637	T1458	T1457	amod	permanent,fixture
R6370	T10153	T10154	compound	follicle,keratinocytes
R6371	T10154	T10151	nsubj	keratinocytes,react
R6372	T10155	T10154	prep	at,keratinocytes
R6373	T10156	T10157	det	this,stage
R6374	T10157	T10155	pobj	stage,at
R6375	T10158	T10157	amod	early,stage
R6376	T10159	T10157	prep	of,stage
R6377	T10160	T10159	pobj	development,of
R6378	T10161	T10151	prep	to,react
R6379	T10162	T10163	compound	TGF,β2
R638	T1459	T1457	prep	of,fixture
R6380	T10163	T10165	compound	β2,signaling
R6381	T10164	T10163	punct	-,β2
R6382	T10165	T10161	pobj	signaling,to
R6383	T10166	T10151	prep	in,react
R6384	T10167	T10168	det	a,fashion
R6385	T10168	T10166	pobj	fashion,in
R6386	T10169	T10168	amod	opposite,fashion
R6387	T10170	T10169	prep	to,opposite
R6388	T10171	T10170	pobj	that,to
R6389	T10172	T10173	advmod	typically,expected
R639	T1460	T1461	det	the,follicle
R6390	T10173	T10171	acl	expected,that
R6391	T10174	T10173	prep	for,expected
R6392	T10175	T10176	compound	TGF,β
R6393	T10176	T10178	compound	β,factors
R6394	T10177	T10176	punct	-,β
R6395	T10178	T10174	pobj	factors,for
R6396	T10179	T10132	punct	.,lends
R6397	T10181	T10182	nsubj	This,said
R6398	T10182	T10183	advcl	said,appeared
R6399	T10184	T10183	punct	", ",appeared
R64	T216	T214	dobj	dynamics,orchestrating
R640	T1461	T1459	pobj	follicle,of
R6400	T10185	T10183	prep	based,appeared
R6401	T10186	T10185	prep	upon,based
R6402	T10187	T10188	compound	pSMAD2,immunohistochemistry
R6403	T10188	T10186	pobj	immunohistochemistry,upon
R6404	T10189	T10183	punct	", ",appeared
R6405	T10190	T10191	det	the,steps
R6406	T10191	T10183	nsubj	steps,appeared
R6407	T10192	T10191	amod	immediate,steps
R6408	T10193	T10191	prep	of,steps
R6409	T10194	T10195	amod	downstream,signaling
R641	T1462	T1461	compound	hair,follicle
R6410	T10195	T10193	pobj	signaling,of
R6411	T10196	T10197	aux	to,be
R6412	T10197	T10183	xcomp	be,appeared
R6413	T10198	T10197	acomp	intact,be
R6414	T10199	T10183	punct	.,appeared
R6415	T10201	T10202	advmod	Thus,surmise
R6416	T10203	T10202	punct	", ",surmise
R6417	T10204	T10202	nsubj	we,surmise
R6418	T10205	T10206	mark	that,is
R6419	T10206	T10202	ccomp	is,surmise
R642	T1463	T1426	punct	.,instructs
R6420	T10207	T10208	det	the,outcome
R6421	T10208	T10206	nsubj	outcome,is
R6422	T10209	T10208	amod	proliferative,outcome
R6423	T10210	T10206	acomp	likely,is
R6424	T10211	T10212	aux	to,rooted
R6425	T10212	T10210	xcomp	rooted,likely
R6426	T10213	T10212	auxpass	be,rooted
R6427	T10214	T10212	prep	in,rooted
R6428	T10215	T10214	pobj	differences,in
R6429	T10216	T10215	prep	in,differences
R643	T1465	T1466	amod	Subsequent,exchanges
R6430	T10217	T10218	det	the,repertoire
R6431	T10218	T10216	pobj	repertoire,in
R6432	T10219	T10218	prep	of,repertoire
R6433	T10220	T10221	amod	activated,genes
R6434	T10221	T10219	pobj	genes,of
R6435	T10222	T10221	compound	SMAD,genes
R6436	T10223	T10221	compound	target,genes
R6437	T10224	T10202	punct	.,surmise
R6438	T10226	T10227	prep	In,be
R6439	T10228	T10229	det	this,regard
R644	T1466	T1467	nsubj	exchanges,result
R6440	T10229	T10226	pobj	regard,In
R6441	T10230	T10227	punct	", ",be
R6442	T10231	T10232	det	the,effects
R6443	T10232	T10227	nsubj	effects,be
R6444	T10233	T10232	amod	positive,effects
R6445	T10234	T10232	prep	of,effects
R6446	T10235	T10236	compound	TGF,β2
R6447	T10236	T10234	pobj	β2,of
R6448	T10237	T10236	punct	-,β2
R6449	T10238	T10232	prep	on,effects
R645	T1468	T1466	prep	between,exchanges
R6450	T10239	T10238	pobj	proliferation,on
R6451	T10240	T10232	prep	within,effects
R6452	T10241	T10242	det	the,bud
R6453	T10242	T10240	pobj	bud,within
R6454	T10243	T10242	compound	hair,bud
R6455	T10244	T10227	aux	may,be
R6456	T10245	T10246	advmod	more,analogous
R6457	T10246	T10227	acomp	analogous,be
R6458	T10247	T10246	prep	to,analogous
R6459	T10248	T10249	dep	what,seen
R646	T1469	T1470	det	the,placode
R6460	T10249	T10247	pobj	seen,to
R6461	T10250	T10249	aux	has,seen
R6462	T10251	T10249	auxpass	been,seen
R6463	T10252	T10249	prep	in,seen
R6464	T10253	T10252	pobj	progression,in
R6465	T10254	T10253	prep	of,progression
R6466	T10255	T10256	amod	squamous,cell
R6467	T10256	T10257	compound	cell,carcinoma
R6468	T10257	T10254	pobj	carcinoma,of
R6469	T10258	T10253	prep	to,progression
R647	T1470	T1468	pobj	placode,between
R6470	T10259	T10260	amod	metastatic,carcinoma
R6471	T10260	T10258	pobj	carcinoma,to
R6472	T10261	T10262	punct	[,43
R6473	T10262	T10249	parataxis	43,seen
R6474	T10263	T10262	punct	],43
R6475	T10264	T10265	advmod	rather,than
R6476	T10265	T10249	cc	than,seen
R6477	T10266	T10249	conj	that,seen
R6478	T10267	T10268	advmod	typically,observed
R6479	T10268	T10266	acl	observed,that
R648	T1471	T1470	cc	and,placode
R6480	T10269	T10268	prep	for,observed
R6481	T10270	T10269	pobj	keratinocytes,for
R6482	T10271	T10272	punct	[,46
R6483	T10272	T10227	parataxis	46,be
R6484	T10273	T10272	nummod	44,46
R6485	T10274	T10272	punct	",",46
R6486	T10275	T10272	nummod	45,46
R6487	T10276	T10272	punct	",",46
R6488	T10277	T10272	punct	],46
R6489	T10278	T10227	punct	.,be
R649	T1472	T1473	amod	nascent,papilla
R6490	T10280	T10281	det	The,identification
R6491	T10281	T10283	nsubj	identification,was
R6492	T10282	T10281	amod	prior,identification
R6493	T10284	T10281	prep	of,identification
R6494	T10285	T10286	det	the,gene
R6495	T10286	T10284	pobj	gene,of
R6496	T10287	T10286	compound	Snail,gene
R6497	T10288	T10281	prep	as,identification
R6498	T10289	T10290	det	a,target
R6499	T10290	T10288	pobj	target,as
R65	T217	T216	cc	and,dynamics
R650	T1473	T1470	conj	papilla,placode
R6500	T10291	T10290	amod	potential,target
R6501	T10292	T10290	prep	of,target
R6502	T10293	T10294	compound	TGF,β
R6503	T10294	T10296	compound	β,signaling
R6504	T10295	T10294	punct	-,β
R6505	T10296	T10292	pobj	signaling,of
R6506	T10297	T10298	punct	[,15
R6507	T10298	T10281	parataxis	15,identification
R6508	T10299	T10298	punct	],15
R6509	T10300	T10283	acomp	intriguing,was
R651	T1474	T1473	amod	dermal,papilla
R6510	T10301	T10283	punct	", ",was
R6511	T10302	T10283	prep	given,was
R6512	T10303	T10304	det	the,wave
R6513	T10304	T10302	pobj	wave,given
R6514	T10305	T10304	amod	temporal,wave
R6515	T10306	T10304	prep	of,wave
R6516	T10307	T10308	compound	Snail,expression
R6517	T10308	T10306	pobj	expression,of
R6518	T10309	T10308	compound	gene,expression
R6519	T10310	T10311	dep	that,occurs
R652	T1475	T1467	prep	in,result
R6520	T10311	T10304	relcl	occurs,wave
R6521	T10312	T10311	prep	in,occurs
R6522	T10313	T10314	det	the,bud
R6523	T10314	T10312	pobj	bud,in
R6524	T10315	T10314	amod	developing,bud
R6525	T10316	T10314	compound	hair,bud
R6526	T10317	T10283	punct	.,was
R6527	T10319	T10320	det	The,correlation
R6528	T10320	T10322	nsubj	correlation,fostered
R6529	T10321	T10320	amod	additional,correlation
R653	T1476	T1477	amod	further,growth
R6530	T10323	T10320	prep	between,correlation
R6531	T10324	T10325	amod	epithelial,hyperproliferation
R6532	T10325	T10323	pobj	hyperproliferation,between
R6533	T10326	T10325	cc	and,hyperproliferation
R6534	T10327	T10328	compound	Snail,expression
R6535	T10328	T10325	conj	expression,hyperproliferation
R6536	T10329	T10328	compound	transgene,expression
R6537	T10330	T10322	advmod	further,fostered
R6538	T10331	T10332	poss	our,interest
R6539	T10332	T10322	dobj	interest,fostered
R654	T1477	T1475	pobj	growth,in
R6540	T10333	T10332	prep	in,interest
R6541	T10334	T10335	det	a,link
R6542	T10335	T10333	pobj	link,in
R6543	T10336	T10335	amod	possible,link
R6544	T10337	T10335	prep	between,link
R6545	T10338	T10339	compound	TGF,β2
R6546	T10339	T10337	pobj	β2,between
R6547	T10340	T10339	punct	-,β2
R6548	T10341	T10339	cc	and,β2
R6549	T10342	T10339	conj	Snail,β2
R655	T1478	T1477	prep	of,growth
R6550	T10343	T10322	punct	.,fostered
R6551	T10345	T10346	poss	Our,studies
R6552	T10346	T10348	nsubj	studies,demonstrate
R6553	T10347	T10346	amod	functional,studies
R6554	T10349	T10350	mark	that,abolished
R6555	T10350	T10348	ccomp	abolished,demonstrate
R6556	T10351	T10350	prep	without,abolished
R6557	T10352	T10353	compound	TGF,β2
R6558	T10353	T10351	pobj	β2,without
R6559	T10354	T10353	punct	-,β2
R656	T1479	T1480	det	the,follicle
R6560	T10355	T10350	punct	", ",abolished
R6561	T10356	T10357	compound	Snail,expression
R6562	T10357	T10350	nsubjpass	expression,abolished
R6563	T10358	T10350	auxpass	is,abolished
R6564	T10359	T10350	prep	in,abolished
R6565	T10360	T10361	det	the,buds
R6566	T10361	T10359	pobj	buds,in
R6567	T10362	T10361	compound	mutant,buds
R6568	T10363	T10361	compound	hair,buds
R6569	T10364	T10350	punct	", ",abolished
R657	T1480	T1478	pobj	follicle,of
R6570	T10365	T10350	cc	and,abolished
R6571	T10366	T10367	advmod	conversely,activated
R6572	T10367	T10350	conj	activated,abolished
R6573	T10368	T10367	punct	", ",activated
R6574	T10369	T10367	prep	in,activated
R6575	T10370	T10371	compound	K14,Smad2
R6576	T10371	T10373	compound	Smad2,skin
R6577	T10372	T10371	punct	-,Smad2
R6578	T10373	T10369	pobj	skin,in
R6579	T10374	T10367	punct	", ",activated
R658	T1481	T1477	prep	into,growth
R6580	T10375	T10367	nsubjpass	Snail,activated
R6581	T10376	T10367	auxpass	is,activated
R6582	T10377	T10367	advmod	ectopically,activated
R6583	T10378	T10348	punct	.,demonstrate
R6584	T10380	T10381	advmod	Moreover,indicate
R6585	T10382	T10381	punct	", ",indicate
R6586	T10383	T10384	poss	our,studies
R6587	T10384	T10381	nsubj	studies,indicate
R6588	T10385	T10386	advmod	in,vitro
R6589	T10386	T10384	amod	vitro,studies
R659	T1482	T1483	det	the,dermis
R6590	T10387	T10388	mark	that,cause
R6591	T10388	T10381	ccomp	cause,indicate
R6592	T10389	T10390	advmod	even,exposure
R6593	T10390	T10388	nsubj	exposure,cause
R6594	T10391	T10390	amod	sustained,exposure
R6595	T10392	T10393	compound	TGF,β2
R6596	T10393	T10390	compound	β2,exposure
R6597	T10394	T10393	punct	-,β2
R6598	T10395	T10388	aux	may,cause
R6599	T10396	T10397	advmod	only,induction
R66	T218	T216	conj	proliferation,dynamics
R660	T1483	T1481	pobj	dermis,into
R6600	T10397	T10388	dobj	induction,cause
R6601	T10398	T10397	det	a,induction
R6602	T10399	T10397	amod	transient,induction
R6603	T10400	T10397	prep	of,induction
R6604	T10401	T10400	pobj	Snail,of
R6605	T10402	T10381	punct	", ",indicate
R6606	T10403	T10381	advcl	offering,indicate
R6607	T10404	T10405	det	a,explanation
R6608	T10405	T10403	dobj	explanation,offering
R6609	T10406	T10405	amod	possible,explanation
R661	T1484	T1483	amod	underlying,dermis
R6610	T10407	T10405	prep	as,explanation
R6611	T10408	T10407	prep	to,as
R6612	T10409	T10410	advmod	why,expressed
R6613	T10410	T10408	pcomp	expressed,to
R6614	T10411	T10410	nsubjpass	Snail,expressed
R6615	T10412	T10410	auxpass	is,expressed
R6616	T10413	T10414	advmod	so,briefly
R6617	T10414	T10410	advmod	briefly,expressed
R6618	T10415	T10410	prep	during,expressed
R6619	T10416	T10417	compound	hair,follicle
R662	T1485	T1477	punct	", ",growth
R6620	T10417	T10418	compound	follicle,morphogenesis
R6621	T10418	T10415	pobj	morphogenesis,during
R6622	T10419	T10381	punct	.,indicate
R6623	T10421	T10422	det	An,point
R6624	T10422	T10424	nsubj	point,is
R6625	T10423	T10422	amod	additional,point
R6626	T10425	T10422	amod	worth,point
R6627	T10426	T10425	advcl	mentioning,worth
R6628	T10427	T10428	mark	that,resulted
R6629	T10428	T10424	ccomp	resulted,is
R663	T1486	T1477	cc	or,growth
R6630	T10429	T10430	amod	prolonged,expression
R6631	T10430	T10428	nsubj	expression,resulted
R6632	T10431	T10430	prep	of,expression
R6633	T10432	T10433	compound	Tg,Snail
R6634	T10433	T10431	pobj	Snail,of
R6635	T10434	T10430	prep	in,expression
R6636	T10435	T10436	amod	postnatal,skin
R6637	T10436	T10434	pobj	skin,in
R6638	T10437	T10428	prep	in,resulted
R6639	T10438	T10439	amod	morphological,signs
R664	T1487	T1488	amod	down,growth
R6640	T10439	T10437	pobj	signs,in
R6641	T10440	T10438	cc	and,morphological
R6642	T10441	T10438	conj	biochemical,morphological
R6643	T10442	T10439	prep	of,signs
R6644	T10443	T10444	amod	epithelial,transitions
R6645	T10444	T10442	pobj	transitions,of
R6646	T10445	T10443	prep	to,epithelial
R6647	T10446	T10445	amod	mesenchymal,to
R6648	T10447	T10448	punct	(,data
R6649	T10448	T10428	meta	data,resulted
R665	T1488	T1477	conj	growth,growth
R6650	T10449	T10448	amod	unpublished,data
R6651	T10450	T10448	punct	),data
R6652	T10451	T10428	punct	", ",resulted
R6653	T10452	T10428	advcl	underscoring,resulted
R6654	T10453	T10454	advmod	why,be
R6655	T10454	T10452	ccomp	be,underscoring
R6656	T10455	T10456	amod	transient,expression
R6657	T10456	T10454	nsubj	expression,be
R6658	T10457	T10456	compound	Snail,expression
R6659	T10458	T10454	aux	may,be
R666	T1489	T1488	punct	-,growth
R6660	T10459	T10460	advmod	so,important
R6661	T10460	T10454	acomp	important,be
R6662	T10461	T10454	prep	during,be
R6663	T10462	T10463	amod	normal,morphogenesis
R6664	T10463	T10461	pobj	morphogenesis,during
R6665	T10464	T10465	compound	hair,follicle
R6666	T10465	T10463	compound	follicle,morphogenesis
R6667	T10466	T10467	punct	[,18
R6668	T10467	T10424	parataxis	18,is
R6669	T10468	T10467	punct	],18
R667	T1490	T1488	punct	", ",growth
R6670	T10469	T10424	punct	.,is
R6671	T10471	T10472	prep	At,seemed
R6672	T10473	T10474	amod	first,glance
R6673	T10474	T10471	pobj	glance,At
R6674	T10475	T10472	punct	", ",seemed
R6675	T10476	T10477	det	the,sparsity
R6676	T10477	T10472	nsubj	sparsity,seemed
R6677	T10478	T10477	prep	in,sparsity
R6678	T10479	T10480	compound	hair,coat
R6679	T10480	T10478	pobj	coat,in
R668	T1491	T1488	cc	and,growth
R6680	T10481	T10480	prep	of,coat
R6681	T10482	T10483	compound	K14,Snail
R6682	T10483	T10485	compound	Snail,mice
R6683	T10484	T10483	punct	-,Snail
R6684	T10485	T10481	pobj	mice,of
R6685	T10486	T10485	compound	Tg,mice
R6686	T10487	T10472	oprd	indicative,seemed
R6687	T10488	T10487	prep	of,indicative
R6688	T10489	T10490	det	a,defect
R6689	T10490	T10488	pobj	defect,of
R669	T1492	T1493	amod	eventual,differentiation
R6690	T10491	T10490	prep	in,defect
R6691	T10492	T10493	compound	follicle,formation
R6692	T10493	T10491	pobj	formation,in
R6693	T10494	T10495	punct	(,see
R6694	T10495	T10472	parataxis	see,seemed
R6695	T10496	T10497	compound	Figure,2A
R6696	T10497	T10495	dobj	2A,see
R6697	T10498	T10495	punct	),see
R6698	T10499	T10472	punct	.,seemed
R6699	T10501	T10502	amod	Closer,inspection
R67	T219	T220	aux	to,shape
R670	T1493	T1488	conj	differentiation,growth
R6700	T10502	T10503	nsubj	inspection,revealed
R6701	T10504	T10503	punct	", ",revealed
R6702	T10505	T10503	advmod	however,revealed
R6703	T10506	T10503	punct	", ",revealed
R6704	T10507	T10508	mark	that,penetrated
R6705	T10508	T10503	ccomp	penetrated,revealed
R6706	T10509	T10510	neg	not,hairs
R6707	T10510	T10508	nsubj	hairs,penetrated
R6708	T10511	T10510	det	all,hairs
R6709	T10512	T10513	det	the,epidermis
R671	T1494	T1493	prep	into,differentiation
R6710	T10513	T10508	dobj	epidermis,penetrated
R6711	T10514	T10513	amod	hyperthickened,epidermis
R6712	T10515	T10513	compound	Tg,epidermis
R6713	T10516	T10503	punct	.,revealed
R6714	T10518	T10519	amod	Several,factors
R6715	T10519	T10520	nsubj	factors,contribute
R6716	T10521	T10520	aux	may,contribute
R6717	T10522	T10520	prep	to,contribute
R6718	T10523	T10524	det	the,development
R6719	T10524	T10522	pobj	development,to
R672	T1495	T1496	det	the,layers
R6720	T10525	T10526	advmod	seemingly,normal
R6721	T10526	T10524	amod	normal,development
R6722	T10527	T10524	compound	follicle,development
R6723	T10528	T10524	prep	in,development
R6724	T10529	T10530	det	these,mice
R6725	T10530	T10528	pobj	mice,in
R6726	T10531	T10520	punct	.,contribute
R6727	T10533	T10534	nummod	One,factor
R6728	T10534	T10536	nsubj	factor,is
R6729	T10535	T10534	amod	obvious,factor
R673	T1496	T1494	pobj	layers,into
R6730	T10537	T10538	det	the,promoter
R6731	T10538	T10536	attr	promoter,is
R6732	T10539	T10538	compound	K14,promoter
R6733	T10540	T10538	punct	", ",promoter
R6734	T10541	T10542	dep	which,is
R6735	T10542	T10538	relcl	is,promoter
R6736	T10543	T10542	acomp	elevated,is
R6737	T10544	T10542	prep	in,is
R6738	T10545	T10546	det	the,layer
R6739	T10546	T10544	pobj	layer,in
R674	T1497	T1496	nummod	six,layers
R6740	T10547	T10546	amod	basal,layer
R6741	T10548	T10546	prep	of,layer
R6742	T10549	T10550	det	the,epidermis
R6743	T10550	T10548	pobj	epidermis,of
R6744	T10551	T10546	cc	and,layer
R6745	T10552	T10553	det	the,sheath
R6746	T10553	T10546	conj	sheath,layer
R6747	T10554	T10553	amod	outer,sheath
R6748	T10555	T10553	compound	root,sheath
R6749	T10556	T10553	punct	(,sheath
R675	T1498	T1496	amod	concentric,layers
R6750	T10557	T10553	appos	ORS,sheath
R6751	T10558	T10553	punct	),sheath
R6752	T10559	T10553	prep	of,sheath
R6753	T10560	T10561	det	the,follicle
R6754	T10561	T10559	pobj	follicle,of
R6755	T10562	T10561	compound	hair,follicle
R6756	T10563	T10536	punct	", ",is
R6757	T10564	T10536	cc	but,is
R6758	T10565	T10566	auxpass	is,regulated
R6759	T10566	T10536	conj	regulated,is
R676	T1499	T1496	prep	of,layers
R6760	T10567	T10566	advmod	markedly,regulated
R6761	T10568	T10566	advmod	down,regulated
R6762	T10569	T10566	punct	-,regulated
R6763	T10570	T10566	prep	in,regulated
R6764	T10571	T10572	amod	developing,buds
R6765	T10572	T10570	pobj	buds,in
R6766	T10573	T10572	amod	embryonic,buds
R6767	T10574	T10572	compound	hair,buds
R6768	T10575	T10576	advmod	as,as
R6769	T10576	T10570	cc	as,in
R677	T1500	T1501	det	the,follicle
R6770	T10577	T10576	advmod	well,as
R6771	T10578	T10570	conj	in,in
R6772	T10579	T10580	det	the,cells
R6773	T10580	T10578	pobj	cells,in
R6774	T10581	T10580	amod	postnatal,cells
R6775	T10582	T10580	compound	hair,cells
R6776	T10583	T10580	compound	progenitor,cells
R6777	T10584	T10536	punct	.,is
R6778	T10586	T10587	det	The,promoter
R6779	T10587	T10589	nsubj	promoter,is
R678	T1501	T1499	pobj	follicle,of
R6780	T10588	T10587	compound	K14,promoter
R6781	T10590	T10589	advmod	also,is
R6782	T10591	T10592	advmod	less,active
R6783	T10592	T10589	acomp	active,is
R6784	T10593	T10592	prep	in,active
R6785	T10594	T10595	det	the,ORS
R6786	T10595	T10593	pobj	ORS,in
R6787	T10596	T10592	prep	than,active
R6788	T10597	T10596	pcomp	epidermis,than
R6789	T10598	T10589	cc	and,is
R679	T1502	T1501	amod	mature,follicle
R6790	T10599	T10600	advmod	hence,account
R6791	T10600	T10589	conj	account,is
R6792	T10601	T10600	nsubj	this,account
R6793	T10602	T10600	aux	might,account
R6794	T10603	T10600	advmod	also,account
R6795	T10604	T10600	prep	for,account
R6796	T10605	T10606	det	the,lack
R6797	T10606	T10604	pobj	lack,for
R6798	T10607	T10606	prep	of,lack
R6799	T10608	T10609	amod	apparent,response
R68	T220	T214	advcl	shape,orchestrating
R680	T1503	T1467	punct	.,result
R6800	T10609	T10607	pobj	response,of
R6801	T10610	T10609	prep	of,response
R6802	T10611	T10612	det	the,ORS
R6803	T10612	T10610	pobj	ORS,of
R6804	T10613	T10609	prep	to,response
R6805	T10614	T10615	amod	ectopic,Snail
R6806	T10615	T10613	pobj	Snail,to
R6807	T10616	T10600	punct	.,account
R6808	T10618	T10619	amod	Additional,factors
R6809	T10619	T10621	nsubj	factors,be
R681	T1505	T1506	advmod	Previously,delineated
R6810	T10620	T10619	amod	contributing,factors
R6811	T10622	T10621	aux	could,be
R6812	T10623	T10624	det	the,multiplicity
R6813	T10624	T10621	attr	multiplicity,be
R6814	T10625	T10624	prep	of,multiplicity
R6815	T10626	T10627	compound	Snail,members
R6816	T10627	T10625	pobj	members,of
R6817	T10628	T10627	compound	family,members
R6818	T10629	T10627	cc	and,members
R6819	T10630	T10631	poss	their,expression
R682	T1507	T1506	punct	", ",delineated
R6820	T10631	T10627	conj	expression,members
R6821	T10632	T10631	amod	differential,expression
R6822	T10633	T10627	punct	", ",members
R6823	T10634	T10635	det	the,saturation
R6824	T10635	T10627	appos	saturation,members
R6825	T10636	T10635	punct	", ",saturation
R6826	T10637	T10635	cc	and,saturation
R6827	T10638	T10637	punct	/,and
R6828	T10639	T10637	cc	or,and
R6829	T10640	T10635	conj	diversity,saturation
R683	T1508	T1506	nsubj	we,delineated
R6830	T10641	T10635	prep	of,saturation
R6831	T10642	T10643	amod	regulatory,mechanisms
R6832	T10643	T10641	pobj	mechanisms,of
R6833	T10644	T10645	dep	that,govern
R6834	T10645	T10643	relcl	govern,mechanisms
R6835	T10646	T10647	compound	AJ,formation
R6836	T10647	T10645	dobj	formation,govern
R6837	T10648	T10647	punct	", ",formation
R6838	T10649	T10647	conj	migration,formation
R6839	T10650	T10649	punct	", ",migration
R684	T1509	T1510	advmod	how,function
R6840	T10651	T10649	cc	and,migration
R6841	T10652	T10649	conj	proliferation,migration
R6842	T10653	T10645	prep	in,govern
R6843	T10654	T10655	det	the,ORS
R6844	T10655	T10653	pobj	ORS,in
R6845	T10656	T10655	compound	follicle,ORS
R6846	T10657	T10621	punct	.,be
R6847	T10659	T10660	csubj	Distinguishing,await
R6848	T10661	T10659	prep	between,Distinguishing
R6849	T10662	T10663	det	these,possibilities
R685	T1510	T1506	ccomp	function,delineated
R6850	T10663	T10661	pobj	possibilities,between
R6851	T10664	T10660	aux	must,await
R6852	T10665	T10666	det	the,generation
R6853	T10666	T10660	dobj	generation,await
R6854	T10667	T10666	prep	of,generation
R6855	T10668	T10667	pobj	mice,of
R6856	T10669	T10668	acl	harboring,mice
R6857	T10670	T10671	npadvmod	skin,specific
R6858	T10671	T10673	amod	specific,mutations
R6859	T10672	T10671	punct	-,specific
R686	T1511	T1512	nummod	two,signals
R6860	T10673	T10669	dobj	mutations,harboring
R6861	T10674	T10673	nmod	loss,mutations
R6862	T10675	T10674	punct	-,loss
R6863	T10676	T10674	prep	of,loss
R6864	T10677	T10676	punct	-,of
R6865	T10678	T10676	pobj	function,of
R6866	T10679	T10673	compound	Snail,mutations
R6867	T10680	T10660	punct	.,await
R687	T1512	T1510	nsubj	signals,function
R6876	T11058	T11057	prep	between,Links
R6877	T11059	T11058	pobj	Signaling,between
R6878	T11060	T11059	punct	", ",Signaling
R6879	T11061	T11062	amod	Transcriptional,Cascades
R688	T1513	T1512	amod	respective,signals
R6880	T11062	T11059	conj	Cascades,Signaling
R6881	T11063	T11062	punct	", ",Cascades
R6882	T11064	T11065	amod	Epithelial,Remodeling
R6883	T11065	T11062	conj	Remodeling,Cascades
R6884	T11066	T11065	punct	", ",Remodeling
R6885	T11067	T11065	cc	and,Remodeling
R6886	T11068	T11065	conj	Proliferation,Remodeling
R6887	T11069	T11057	prep	in,Links
R6888	T11070	T11071	det	the,Bud
R6889	T11071	T11069	pobj	Bud,in
R689	T1514	T1512	amod	epithelial,signals
R6890	T11072	T11071	compound	Hair,Bud
R6891	T11074	T11075	advmod	Previously,discovered
R6892	T11076	T11075	punct	", ",discovered
R6893	T11077	T11075	nsubj	we,discovered
R6894	T11078	T11079	mark	that,regulated
R6895	T11079	T11075	ccomp	regulated,discovered
R6896	T11080	T11079	advmod	early,regulated
R6897	T11081	T11080	prep	during,early
R6898	T11082	T11083	compound	hair,follicle
R6899	T11083	T11084	compound	follicle,morphogenesis
R69	T221	T222	det	the,cluster
R690	T1515	T1514	cc	and,epithelial
R6900	T11084	T11081	pobj	morphogenesis,during
R6901	T11085	T11079	punct	", ",regulated
R6902	T11086	T11087	compound	E,cadherin
R6903	T11087	T11089	compound	cadherin,expression
R6904	T11088	T11087	punct	-,cadherin
R6905	T11089	T11079	nsubjpass	expression,regulated
R6906	T11090	T11089	compound	gene,expression
R6907	T11091	T11079	auxpass	is,regulated
R6908	T11092	T11079	advmod	down,regulated
R6909	T11093	T11079	punct	-,regulated
R691	T1516	T1514	conj	mesenchymal,epithelial
R6910	T11094	T11079	advmod	concomitantly,regulated
R6911	T11095	T11079	prep	with,regulated
R6912	T11096	T11097	det	the,invagination
R6913	T11097	T11095	pobj	invagination,with
R6914	T11098	T11097	prep	of,invagination
R6915	T11099	T11100	amod	developing,cells
R6916	T11100	T11098	pobj	cells,of
R6917	T11101	T11100	compound	bud,cells
R6918	T11102	T11097	prep	into,invagination
R6919	T11103	T11104	det	the,skin
R692	T1517	T1512	punct	", ",signals
R6920	T11104	T11102	pobj	skin,into
R6921	T11105	T11106	punct	[,4
R6922	T11106	T11075	parataxis	4,discovered
R6923	T11107	T11106	punct	],4
R6924	T11108	T11075	punct	.,discovered
R6925	T11110	T11111	mark	Because,correlated
R6926	T11111	T11117	advcl	correlated,intrigued
R6927	T11112	T11113	det	the,timing
R6928	T11113	T11111	nsubj	timing,correlated
R6929	T11114	T11113	prep	of,timing
R693	T1518	T1512	appos	Wnts,signals
R6930	T11115	T11116	det	this,event
R6931	T11116	T11114	pobj	event,of
R6932	T11118	T11111	prep	with,correlated
R6933	T11119	T11120	det	the,activation
R6934	T11120	T11118	pobj	activation,with
R6935	T11121	T11120	prep	of,activation
R6936	T11122	T11123	det	a,complex
R6937	T11123	T11121	pobj	complex,of
R6938	T11124	T11125	nmod	LEF,factor
R6939	T11125	T11123	compound	factor,complex
R694	T1519	T1518	cc	and,Wnts
R6940	T11126	T11124	punct	-,LEF
R6941	T11127	T11124	nummod	1,LEF
R6942	T11128	T11124	punct	/,LEF
R6943	T11129	T11130	compound	β,catenin
R6944	T11130	T11124	appos	catenin,LEF
R6945	T11131	T11130	punct	-,catenin
R6946	T11132	T11125	compound	transcription,factor
R6947	T11133	T11134	punct	[,20
R6948	T11134	T11111	parataxis	20,correlated
R6949	T11135	T11134	punct	],20
R695	T1520	T1521	det	the,factor
R6950	T11136	T11117	punct	", ",intrigued
R6951	T11137	T11117	nsubjpass	we,intrigued
R6952	T11138	T11117	auxpass	were,intrigued
R6953	T11139	T11117	agent	by,intrigued
R6954	T11140	T11141	det	the,presence
R6955	T11141	T11139	pobj	presence,by
R6956	T11142	T11141	prep	of,presence
R6957	T11143	T11144	det	a,site
R6958	T11144	T11142	pobj	site,of
R6959	T11145	T11144	amod	putative,site
R696	T1521	T1518	conj	factor,Wnts
R6960	T11146	T11144	nmod	LEF,site
R6961	T11147	T11146	punct	-,LEF
R6962	T11148	T11146	nummod	1,LEF
R6963	T11149	T11146	punct	/,LEF
R6964	T11150	T11146	appos	TCF,LEF
R6965	T11151	T11144	compound	binding,site
R6966	T11152	T11141	prep	in,presence
R6967	T11153	T11154	det	the,promoter
R6968	T11154	T11152	pobj	promoter,in
R6969	T11155	T11156	compound	E,cadherin
R697	T1522	T1523	npadvmod	BMP,inhibitory
R6970	T11156	T11154	compound	cadherin,promoter
R6971	T11157	T11156	punct	-,cadherin
R6972	T11158	T11117	punct	.,intrigued
R6973	T11160	T11161	nsubj	This,prompted
R6974	T11162	T11163	det	an,investigation
R6975	T11163	T11161	dobj	investigation,prompted
R6976	T11164	T11165	dep	that,led
R6977	T11165	T11163	relcl	led,investigation
R6978	T11166	T11165	advmod	subsequently,led
R6979	T11167	T11165	prep	to,led
R698	T1523	T1521	amod	inhibitory,factor
R6980	T11168	T11169	poss	our,discovery
R6981	T11169	T11167	pobj	discovery,to
R6982	T11170	T11171	mark	that,contribute
R6983	T11171	T11169	acl	contribute,discovery
R6984	T11172	T11171	nsubj	LEF,contribute
R6985	T11173	T11172	punct	-,LEF
R6986	T11174	T11172	nummod	1,LEF
R6987	T11175	T11172	punct	/,LEF
R6988	T11176	T11177	compound	β,catenin
R6989	T11177	T11172	appos	catenin,LEF
R699	T1524	T1523	punct	-,inhibitory
R6990	T11178	T11177	punct	-,catenin
R6991	T11179	T11171	aux	can,contribute
R6992	T11180	T11171	prep	to,contribute
R6993	T11181	T11180	pobj	repression,to
R6994	T11182	T11181	prep	of,repression
R6995	T11183	T11184	compound	E,cadherin
R6996	T11184	T11186	compound	cadherin,expression
R6997	T11185	T11184	punct	-,cadherin
R6998	T11186	T11182	pobj	expression,of
R6999	T11187	T11186	compound	gene,expression
R7	T154	T152	cc	and,β2
R70	T222	T220	dobj	cluster,shape
R700	T1525	T1521	appos	noggin,factor
R7000	T11188	T11171	prep	in,contribute
R7001	T11189	T11190	compound	skin,keratinocytes
R7002	T11190	T11188	pobj	keratinocytes,in
R7003	T11191	T11192	punct	[,4
R7004	T11192	T11161	parataxis	4,prompted
R7005	T11193	T11192	punct	],4
R7006	T11194	T11161	punct	.,prompted
R7007	T11196	T11197	prep	In,noted
R7008	T11198	T11199	det	the,course
R7009	T11199	T11196	pobj	course,In
R701	T1526	T1510	punct	", ",function
R7010	T11200	T11199	prep	of,course
R7011	T11201	T11202	det	these,studies
R7012	T11202	T11200	pobj	studies,of
R7013	T11203	T11197	punct	", ",noted
R7014	T11204	T11197	nsubj	we,noted
R7015	T11205	T11197	advmod	also,noted
R7016	T11206	T11207	mark	that,contribute
R7017	T11207	T11197	ccomp	contribute,noted
R7018	T11208	T11207	nsubj	Snail,contribute
R7019	T11209	T11207	aux	can,contribute
R702	T1527	T1510	prep	in,function
R7020	T11210	T11207	advmod	also,contribute
R7021	T11211	T11207	prep	to,contribute
R7022	T11212	T11213	det	this,process
R7023	T11213	T11211	pobj	process,to
R7024	T11214	T11207	prep	in,contribute
R7025	T11215	T11214	pobj	keratinocytes,in
R7026	T11216	T11217	advmod	in,vitro
R7027	T11217	T11207	advmod	vitro,contribute
R7028	T11218	T11197	punct	", ",noted
R7029	T11219	T11197	cc	and,noted
R703	T1528	T1527	pobj	concert,in
R7030	T11220	T11221	poss	our,studies
R7031	T11221	T11223	nsubj	studies,revealed
R7032	T11222	T11221	amod	present,studies
R7033	T11223	T11197	conj	revealed,noted
R7034	T11224	T11225	mark	that,expressed
R7035	T11225	T11223	ccomp	expressed,revealed
R7036	T11226	T11225	nsubjpass	Snail,expressed
R7037	T11227	T11225	auxpass	is,expressed
R7038	T11228	T11225	prep	at,expressed
R7039	T11229	T11230	det	the,place
R704	T1529	T1530	aux	to,induce
R7040	T11230	T11228	pobj	place,at
R7041	T11231	T11230	amod	right,place
R7042	T11232	T11230	cc	and,place
R7043	T11233	T11230	conj	time,place
R7044	T11234	T11235	aux	to,be
R7045	T11235	T11230	advcl	be,place
R7046	T11236	T11237	advmod	physiologically,relevant
R7047	T11237	T11235	acomp	relevant,be
R7048	T11238	T11235	prep	in,be
R7049	T11239	T11240	det	the,process
R705	T1530	T1510	advcl	induce,function
R7050	T11240	T11238	pobj	process,in
R7051	T11241	T11223	punct	.,revealed
R7052	T11243	T11244	prep	In,abrogated
R7053	T11245	T11246	npadvmod	noggin,null
R7054	T11246	T11248	amod	null,skin
R7055	T11247	T11246	punct	-,null
R7056	T11248	T11243	pobj	skin,In
R7057	T11249	T11248	amod	embryonic,skin
R7058	T11250	T11244	punct	", ",abrogated
R7059	T11251	T11252	nmod	LEF,expression
R706	T1531	T1532	amod	lymphoid,factor
R7060	T11252	T11244	nsubjpass	expression,abrogated
R7061	T11253	T11251	punct	-,LEF
R7062	T11254	T11251	nummod	1,LEF
R7063	T11255	T11252	cc	and,expression
R7064	T11256	T11257	amod	subsequent,activation
R7065	T11257	T11252	conj	activation,expression
R7066	T11258	T11257	prep	of,activation
R7067	T11259	T11260	det	the,gene
R7068	T11260	T11258	pobj	gene,of
R7069	T11261	T11260	nmod	LEF,gene
R707	T1532	T1534	npadvmod	factor,mediated
R7070	T11262	T11261	punct	-,LEF
R7071	T11263	T11261	nummod	1,LEF
R7072	T11264	T11261	punct	/,LEF
R7073	T11265	T11266	compound	β,catenin
R7074	T11266	T11261	appos	catenin,LEF
R7075	T11267	T11266	punct	-,catenin
R7076	T11268	T11260	compound	reporter,gene
R7077	T11269	T11244	auxpass	is,abrogated
R7078	T11270	T11244	prep	in,abrogated
R7079	T11271	T11272	det	the,placodes
R708	T1533	T1532	compound	enhancer,factor
R7080	T11272	T11270	pobj	placodes,in
R7081	T11273	T11272	amod	developing,placodes
R7082	T11274	T11244	punct	.,abrogated
R7083	T11276	T11277	det	The,failure
R7084	T11277	T11279	nsubj	failure,underscores
R7085	T11278	T11277	amod	corresponding,failure
R7086	T11280	T11277	prep	of,failure
R7087	T11281	T11282	nmod	E,cadherin
R7088	T11282	T11284	nmod	cadherin,regulation
R7089	T11283	T11282	punct	-,cadherin
R709	T1534	T1551	amod	mediated,transcription
R7090	T11284	T11280	pobj	regulation,of
R7091	T11285	T11284	amod	down,regulation
R7092	T11286	T11284	punct	-,regulation
R7093	T11287	T11288	det	the,importance
R7094	T11288	T11279	dobj	importance,underscores
R7095	T11289	T11288	prep	of,importance
R7096	T11290	T11291	compound	Wnt,noggin
R7097	T11291	T11293	compound	noggin,signaling
R7098	T11292	T11291	punct	/,noggin
R7099	T11293	T11289	pobj	signaling,of
R71	T223	T222	prep	of,cluster
R710	T1535	T1532	punct	-,factor
R7100	T11294	T11288	prep	in,importance
R7101	T11295	T11294	pcomp	regulating,in
R7102	T11296	T11297	det	this,event
R7103	T11297	T11295	dobj	event,regulating
R7104	T11298	T11295	prep	in,regulating
R7105	T11299	T11300	compound	follicle,morphogenesis
R7106	T11300	T11298	pobj	morphogenesis,in
R7107	T11301	T11302	punct	[,4
R7108	T11302	T11279	parataxis	4,underscores
R7109	T11303	T11302	punct	],4
R711	T1536	T1532	nummod	1,factor
R7110	T11304	T11279	punct	.,underscores
R7111	T11306	T11307	amod	Conditional,studies
R7112	T11307	T11310	nsubj	studies,be
R7113	T11308	T11309	compound	gene,targeting
R7114	T11309	T11307	compound	targeting,studies
R7115	T11311	T11310	aux	will,be
R7116	T11312	T11310	acomp	necessary,be
R7117	T11313	T11314	aux	to,establish
R7118	T11314	T11310	advcl	establish,be
R7119	T11315	T11316	mark	whether,contribute
R712	T1537	T1532	punct	/,factor
R7120	T11316	T11314	ccomp	contribute,establish
R7121	T11317	T11318	compound	Snail,members
R7122	T11318	T11316	nsubj	members,contribute
R7123	T11319	T11318	compound	family,members
R7124	T11320	T11316	advmod	also,contribute
R7125	T11321	T11316	prep	to,contribute
R7126	T11322	T11323	det	the,regulation
R7127	T11323	T11321	pobj	regulation,to
R7128	T11324	T11323	amod	down,regulation
R7129	T11325	T11323	punct	-,regulation
R713	T1538	T1539	compound	β,catenin
R7130	T11326	T11323	prep	in,regulation
R7131	T11327	T11328	compound	E,cadherin
R7132	T11328	T11330	compound	cadherin,expression
R7133	T11329	T11328	punct	-,cadherin
R7134	T11330	T11326	pobj	expression,in
R7135	T11331	T11330	compound	gene,expression
R7136	T11332	T11333	dep	that,occurs
R7137	T11333	T11323	relcl	occurs,regulation
R7138	T11334	T11333	prep	during,occurs
R7139	T11335	T11336	compound	follicle,formation
R714	T1539	T1532	appos	catenin,factor
R7140	T11336	T11334	pobj	formation,during
R7141	T11337	T11310	punct	.,be
R7142	T11339	T11340	advmod	However,is
R7143	T11341	T11340	punct	", ",is
R7144	T11342	T11340	nsubj	it,is
R7145	T11343	T11340	acomp	intriguing,is
R7146	T11344	T11345	mark	that,displayed
R7147	T11345	T11340	ccomp	displayed,is
R7148	T11346	T11347	compound	K14,Snail
R7149	T11347	T11349	compound	Snail,epidermis
R715	T1540	T1539	punct	-,catenin
R7150	T11348	T11347	punct	-,Snail
R7151	T11349	T11345	nsubj	epidermis,displayed
R7152	T11350	T11349	compound	Tg,epidermis
R7153	T11351	T11352	det	a,regulation
R7154	T11352	T11345	dobj	regulation,displayed
R7155	T11353	T11352	amod	marked,regulation
R7156	T11354	T11352	amod	down,regulation
R7157	T11355	T11352	punct	-,regulation
R7158	T11356	T11352	prep	in,regulation
R7159	T11357	T11358	compound	E,cadherin
R716	T1541	T1532	punct	(,factor
R7160	T11358	T11360	compound	cadherin,expression
R7161	T11359	T11358	punct	-,cadherin
R7162	T11360	T11356	pobj	expression,in
R7163	T11361	T11345	punct	", ",displayed
R7164	T11362	T11363	advmod	thereby,demonstrating
R7165	T11363	T11345	advcl	demonstrating,displayed
R7166	T11364	T11365	poss	its,potential
R7167	T11365	T11363	dobj	potential,demonstrating
R7168	T11366	T11367	aux	to,do
R7169	T11367	T11365	acl	do,potential
R717	T1542	T1532	appos	LEF,factor
R7170	T11368	T11367	advmod	so,do
R7171	T11369	T11367	prep	in,do
R7172	T11370	T11369	pobj	skin,in
R7173	T11371	T11340	punct	.,is
R7174	T11373	T11374	poss	Our,findings
R7175	T11374	T11376	nsubj	findings,showed
R7176	T11375	T11374	amod	prior,findings
R7177	T11377	T11378	mark	that,impaired
R7178	T11378	T11376	ccomp	impaired,showed
R7179	T11379	T11378	prep	by,impaired
R718	T1543	T1542	punct	-,LEF
R7180	T11380	T11379	pcomp	elevating,by
R7181	T11381	T11382	compound	E,cadherin
R7182	T11382	T11384	compound	cadherin,levels
R7183	T11383	T11382	punct	-,cadherin
R7184	T11384	T11380	dobj	levels,elevating
R7185	T11385	T11379	cc	or,by
R7186	T11386	T11379	conj	by,by
R7187	T11387	T11388	advmod	conditionally,ablating
R7188	T11388	T11386	pcomp	ablating,by
R7189	T11389	T11390	compound	α,catenin
R719	T1544	T1542	nummod	1,LEF
R7190	T11390	T11388	dobj	catenin,ablating
R7191	T11391	T11390	punct	-,catenin
R7192	T11392	T11378	punct	", ",impaired
R7193	T11393	T11394	compound	hair,follicle
R7194	T11394	T11395	compound	follicle,morphogenesis
R7195	T11395	T11378	nsubjpass	morphogenesis,impaired
R7196	T11396	T11378	aux	can,impaired
R7197	T11397	T11378	auxpass	be,impaired
R7198	T11398	T11399	punct	[,7
R7199	T11399	T11376	parataxis	7,showed
R72	T224	T223	pobj	cells,of
R720	T1545	T1542	punct	/,LEF
R7200	T11400	T11399	nummod	4,7
R7201	T11401	T11399	punct	",",7
R7202	T11402	T11399	punct	],7
R7203	T11403	T11376	punct	.,showed
R7204	T11405	T11406	det	The,hyperproliferation
R7205	T11406	T11409	nsubj	hyperproliferation,led
R7206	T11407	T11406	amod	marked,hyperproliferation
R7207	T11408	T11406	amod	epidermal,hyperproliferation
R7208	T11410	T11406	acl	seen,hyperproliferation
R7209	T11411	T11410	prep	in,seen
R721	T1546	T1547	compound	β,catenin
R7210	T11412	T11413	det	the,skin
R7211	T11413	T11411	pobj	skin,in
R7212	T11414	T11415	compound	K14,Snail
R7213	T11415	T11413	compound	Snail,skin
R7214	T11416	T11415	punct	-,Snail
R7215	T11417	T11413	compound	Tg,skin
R7216	T11418	T11406	punct	", ",hyperproliferation
R7217	T11419	T11406	acl	coupled,hyperproliferation
R7218	T11420	T11419	prep	with,coupled
R7219	T11421	T11422	det	the,suppression
R722	T1547	T1542	appos	catenin,LEF
R7220	T11422	T11420	pobj	suppression,with
R7221	T11423	T11422	amod	converse,suppression
R7222	T11424	T11422	prep	of,suppression
R7223	T11425	T11424	pobj	proliferation,of
R7224	T11426	T11425	cc	and,proliferation
R7225	T11427	T11425	conj	Snail,proliferation
R7226	T11428	T11422	prep	in,suppression
R7227	T11429	T11430	compound	TGF,β2
R7228	T11430	T11432	npadvmod	β2,null
R7229	T11431	T11430	punct	-,β2
R723	T1548	T1547	punct	-,catenin
R7230	T11432	T11434	amod	null,buds
R7231	T11433	T11432	punct	-,null
R7232	T11434	T11428	pobj	buds,in
R7233	T11435	T11434	compound	hair,buds
R7234	T11436	T11409	dobj	us,led
R7235	T11437	T11438	aux	to,wonder
R7236	T11438	T11409	xcomp	wonder,led
R7237	T11439	T11440	mark	whether,have
R7238	T11440	T11438	ccomp	have,wonder
R7239	T11441	T11442	det	the,regulation
R724	T1549	T1534	punct	),mediated
R7240	T11442	T11440	nsubj	regulation,have
R7241	T11443	T11442	amod	down,regulation
R7242	T11444	T11442	punct	-,regulation
R7243	T11445	T11442	prep	of,regulation
R7244	T11446	T11447	compound	E,cadherin
R7245	T11447	T11445	pobj	cadherin,of
R7246	T11448	T11447	punct	-,cadherin
R7247	T11449	T11442	prep	during,regulation
R7248	T11450	T11451	compound	follicle,morphogenesis
R7249	T11451	T11449	pobj	morphogenesis,during
R725	T1550	T1534	punct	-,mediated
R7250	T11452	T11440	aux	might,have
R7251	T11453	T11454	det	a,impact
R7252	T11454	T11440	dobj	impact,have
R7253	T11455	T11454	amod	direct,impact
R7254	T11456	T11454	prep	on,impact
R7255	T11457	T11456	pcomp	elevating,on
R7256	T11458	T11459	det	the,state
R7257	T11459	T11457	dobj	state,elevating
R7258	T11460	T11459	amod	proliferative,state
R7259	T11461	T11459	prep	of,state
R726	T1551	T1530	dobj	transcription,induce
R7260	T11462	T11463	det	these,cells
R7261	T11463	T11461	pobj	cells,of
R7262	T11464	T11409	punct	.,led
R7263	T11466	T11467	poss	Our,studies
R7264	T11467	T11469	nsubj	studies,suggested
R7265	T11468	T11467	compound	Tg,studies
R7266	T11470	T11471	mark	that,is
R7267	T11471	T11469	ccomp	is,suggested
R7268	T11472	T11471	punct	", ",is
R7269	T11473	T11474	advmod	at,least
R727	T1552	T1551	compound	gene,transcription
R7270	T11474	T11475	advmod	least,in
R7271	T11475	T11476	prep	in,through
R7272	T11476	T11471	prep	through,is
R7273	T11477	T11475	pobj	part,in
R7274	T11478	T11479	poss	its,regulation
R7275	T11479	T11476	pobj	regulation,through
R7276	T11480	T11479	prep	of,regulation
R7277	T11481	T11482	compound	E,cadherin
R7278	T11482	T11480	pobj	cadherin,of
R7279	T11483	T11482	punct	-,cadherin
R728	T1553	T1530	prep	within,induce
R7280	T11484	T11471	punct	", ",is
R7281	T11485	T11471	nsubj	Snail,is
R7282	T11486	T11471	acomp	able,is
R7283	T11487	T11488	aux	to,influence
R7284	T11488	T11486	xcomp	influence,able
R7285	T11489	T11490	det	the,localization
R7286	T11490	T11488	dobj	localization,influence
R7287	T11491	T11490	amod	subcellular,localization
R7288	T11492	T11490	prep	of,localization
R7289	T11493	T11494	det	a,variety
R729	T1554	T1555	det	the,placode
R7290	T11494	T11492	pobj	variety,of
R7291	T11495	T11494	prep	of,variety
R7292	T11496	T11497	npadvmod	AJ,associated
R7293	T11497	T11499	amod	associated,proteins
R7294	T11498	T11497	punct	-,associated
R7295	T11499	T11495	pobj	proteins,of
R7296	T11500	T11469	punct	.,suggested
R7297	T11502	T11503	nsubj	One,appears
R7298	T11504	T11502	prep	of,One
R7299	T11505	T11504	pobj	these,of
R73	T225	T222	acl	involved,cluster
R730	T1555	T1553	pobj	placode,within
R7300	T11506	T11507	aux	to,be
R7301	T11507	T11503	xcomp	be,appears
R7302	T11508	T11507	attr	Ajuba,be
R7303	T11509	T11508	punct	", ",Ajuba
R7304	T11510	T11511	dep	which,shown
R7305	T11511	T11508	relcl	shown,Ajuba
R7306	T11512	T11511	auxpass	was,shown
R7307	T11513	T11511	advmod	previously,shown
R7308	T11514	T11515	aux	to,have
R7309	T11515	T11511	xcomp	have,shown
R731	T1556	T1555	compound	follicle,placode
R7310	T11516	T11517	det	the,capacity
R7311	T11517	T11515	dobj	capacity,have
R7312	T11518	T11517	amod	dual,capacity
R7313	T11519	T11520	aux	to,bind
R7314	T11520	T11517	acl	bind,capacity
R7315	T11521	T11520	dobj	Grb,bind
R7316	T11522	T11521	punct	-,Grb
R7317	T11523	T11521	nummod	2,Grb
R7318	T11524	T11525	advmod	as,as
R7319	T11525	T11521	cc	as,Grb
R732	T1557	T1558	punct	[,4
R7320	T11526	T11525	advmod	well,as
R7321	T11527	T11528	compound	α,catenin
R7322	T11528	T11521	conj	catenin,Grb
R7323	T11529	T11528	punct	-,catenin
R7324	T11530	T11531	punct	[,10
R7325	T11531	T11503	parataxis	10,appears
R7326	T11532	T11531	nummod	9,10
R7327	T11533	T11531	punct	",",10
R7328	T11534	T11531	punct	],10
R7329	T11535	T11503	punct	.,appears
R733	T1558	T1506	parataxis	4,delineated
R7330	T11537	T11538	poss	Our,studies
R7331	T11538	T11539	nsubj	studies,revealed
R7332	T11540	T11541	mark	that,develops
R7333	T11541	T11539	ccomp	develops,revealed
R7334	T11542	T11541	prep	in,develops
R7335	T11543	T11544	compound	skin,keratinocytes
R7336	T11544	T11542	pobj	keratinocytes,in
R7337	T11545	T11546	dep	that,harbor
R7338	T11546	T11544	advcl	harbor,keratinocytes
R7339	T11547	T11546	preconj	either,harbor
R734	T1559	T1558	punct	],4
R7340	T11548	T11549	det	a,mutation
R7341	T11549	T11546	dobj	mutation,harbor
R7342	T11550	T11549	amod	conditional,mutation
R7343	T11551	T11549	amod	null,mutation
R7344	T11552	T11546	prep	in,harbor
R7345	T11553	T11554	compound	α,catenin
R7346	T11554	T11552	pobj	catenin,in
R7347	T11555	T11554	punct	-,catenin
R7348	T11556	T11546	cc	or,harbor
R7349	T11557	T11558	dep	that,overexpress
R735	T1560	T1506	punct	.,delineated
R7350	T11558	T11546	conj	overexpress,harbor
R7351	T11559	T11558	dobj	Snail,overexpress
R7352	T11560	T11541	punct	", ",develops
R7353	T11561	T11541	nsubj	Ajuba,develops
R7354	T11562	T11563	det	an,interaction
R7355	T11563	T11541	dobj	interaction,develops
R7356	T11564	T11563	prep	with,interaction
R7357	T11565	T11564	pobj	Grb,with
R7358	T11566	T11565	punct	-,Grb
R7359	T11567	T11565	nummod	2,Grb
R736	T1562	T1563	det	The,changes
R7360	T11568	T11569	dep	that,observed
R7361	T11569	T11563	relcl	observed,interaction
R7362	T11570	T11569	auxpass	is,observed
R7363	T11571	T11569	advmod	otherwise,observed
R7364	T11572	T11569	neg	not,observed
R7365	T11573	T11569	prep	in,observed
R7366	T11574	T11575	compound	WT,keratinocytes
R7367	T11575	T11573	pobj	keratinocytes,in
R7368	T11576	T11539	punct	.,revealed
R7369	T11578	T11579	det	The,abilities
R737	T1563	T1565	nsubj	changes,include
R7370	T11579	T11581	nsubj	abilities,suggest
R7371	T11580	T11579	amod	corresponding,abilities
R7372	T11582	T11579	prep	of,abilities
R7373	T11583	T11584	preconj	either,keratinocytes
R7374	T11584	T11582	pobj	keratinocytes,of
R7375	T11585	T11586	npadvmod	Snail,transfected
R7376	T11586	T11584	amod	transfected,keratinocytes
R7377	T11587	T11586	punct	-,transfected
R7378	T11588	T11586	cc	or,transfected
R7379	T11589	T11590	npadvmod	Ajuba,transfected
R738	T1564	T1563	amod	downstream,changes
R7380	T11590	T11586	conj	transfected,transfected
R7381	T11591	T11590	punct	-,transfected
R7382	T11592	T11593	aux	to,exhibit
R7383	T11593	T11579	acl	exhibit,abilities
R7384	T11594	T11595	amod	elevated,activation
R7385	T11595	T11593	dobj	activation,exhibit
R7386	T11596	T11595	prep	of,activation
R7387	T11597	T11598	det	the,pathway
R7388	T11598	T11596	pobj	pathway,of
R7389	T11599	T11600	compound	Ras,MAPK
R739	T1566	T1563	acl	elicited,changes
R7390	T11600	T11598	compound	MAPK,pathway
R7391	T11601	T11600	punct	-,MAPK
R7392	T11602	T11603	mark	that,be
R7393	T11603	T11581	ccomp	be,suggest
R7394	T11604	T11605	det	the,association
R7395	T11605	T11603	nsubj	association,be
R7396	T11606	T11605	nmod	Grb,association
R7397	T11607	T11606	punct	-,Grb
R7398	T11608	T11606	nummod	2,Grb
R7399	T11609	T11605	prep	of,association
R74	T226	T190	punct	.,employed
R740	T1567	T1566	prep	through,elicited
R7400	T11610	T11609	pobj	Ajuba,of
R7401	T11611	T11603	prep	under,be
R7402	T11612	T11611	pobj	conditions,under
R7403	T11613	T11612	prep	of,conditions
R7404	T11614	T11615	amod	reduced,levels
R7405	T11615	T11613	pobj	levels,of
R7406	T11616	T11615	prep	of,levels
R7407	T11617	T11618	compound	AJ,proteins
R7408	T11618	T11616	pobj	proteins,of
R7409	T11619	T11603	aux	may,be
R741	T1568	T1567	pobj	convergence,through
R7410	T11620	T11621	advmod	directly,relevant
R7411	T11621	T11603	acomp	relevant,be
R7412	T11622	T11621	prep	to,relevant
R7413	T11623	T11624	det	the,parallel
R7414	T11624	T11622	pobj	parallel,to
R7415	T11625	T11624	prep	in,parallel
R7416	T11626	T11625	pobj	hyperproliferation,in
R7417	T11627	T11581	punct	.,suggest
R7418	T11629	T11630	prep	In,shown
R7419	T11631	T11632	amod	stable,lines
R742	T1569	T1568	prep	of,convergence
R7420	T11632	T11629	pobj	lines,In
R7421	T11633	T11632	amod	epithelial,lines
R7422	T11634	T11635	punct	(,kidney
R7423	T11635	T11632	parataxis	kidney,lines
R7424	T11636	T11635	advmod	i.e.,kidney
R7425	T11637	T11635	punct	", ",kidney
R7426	T11638	T11639	nmod	Madin,Darby
R7427	T11639	T11635	nmod	Darby,kidney
R7428	T11640	T11639	punct	-,Darby
R7429	T11641	T11635	amod	canine,kidney
R743	T1675	T1677	compound	cell,contact
R7430	T11642	T11635	punct	", ",kidney
R7431	T11643	T11635	cc	or,kidney
R7432	T11644	T11635	conj	MDCK,kidney
R7433	T11645	T11635	punct	),kidney
R7434	T11646	T11632	compound	cell,lines
R7435	T11647	T11630	punct	", ",shown
R7436	T11648	T11630	nsubjpass	Snail,shown
R7437	T11649	T11630	aux	has,shown
R7438	T11650	T11630	auxpass	been,shown
R7439	T11651	T11652	aux	to,block
R744	T1676	T1675	punct	-,cell
R7440	T11652	T11630	xcomp	block,shown
R7441	T11653	T11654	compound	cell,cycle
R7442	T11654	T11655	compound	cycle,progression
R7443	T11655	T11652	dobj	progression,block
R7444	T11656	T11652	cc	and,block
R7445	T11657	T11652	conj	promote,block
R7446	T11658	T11659	nmod	motility,changes
R7447	T11659	T11657	dobj	changes,promote
R7448	T11660	T11658	cc	and,motility
R7449	T11661	T11658	conj	shape,motility
R745	T1677	T1673	pobj	contact,of
R7450	T11662	T11657	prep	for,promote
R7451	T11663	T11662	pobj	invasion,for
R7452	T11664	T11665	punct	[,47
R7453	T11665	T11630	parataxis	47,shown
R7454	T11666	T11665	punct	],47
R7455	T11667	T11630	punct	.,shown
R7456	T11669	T11670	mark	While,are
R7457	T11670	T11675	advcl	are,differ
R7458	T11671	T11672	poss	our,studies
R7459	T11672	T11670	nsubj	studies,are
R746	T1678	T1671	punct	", ",recruit
R7460	T11673	T11674	advmod	in,vivo
R7461	T11674	T11672	amod	vivo,studies
R7462	T11676	T11670	acomp	consistent,are
R7463	T11677	T11676	prep	with,consistent
R7464	T11678	T11679	det	a,role
R7465	T11679	T11677	pobj	role,with
R7466	T11680	T11679	prep	for,role
R7467	T11681	T11680	pobj	Snail,for
R7468	T11682	T11679	prep	in,role
R7469	T11683	T11684	nmod	motility,remodeling
R747	T1679	T1671	advmod	however,recruit
R7470	T11684	T11682	pobj	remodeling,in
R7471	T11685	T11683	cc	and,motility
R7472	T11686	T11683	conj	epithelial,motility
R7473	T11687	T11675	punct	", ",differ
R7474	T11688	T11675	nsubj	they,differ
R7475	T11689	T11675	prep	with,differ
R7476	T11690	T11689	pobj	respect,with
R7477	T11691	T11690	prep	to,respect
R7478	T11692	T11693	poss	Snail,effects
R7479	T11693	T11691	pobj	effects,to
R748	T1570	T1571	det	these,pathways
R7480	T11694	T11692	case	's,Snail
R7481	T11695	T11693	amod	apparent,effects
R7482	T11696	T11693	amod	proliferative,effects
R7483	T11697	T11675	punct	.,differ
R7484	T11699	T11700	advmod	A,priori
R7485	T11700	T11701	advmod	priori,be
R7486	T11702	T11701	punct	", ",be
R7487	T11703	T11701	nsubj	this,be
R7488	T11704	T11701	aux	could,be
R7489	T11705	T11701	advmod	simply,be
R749	T1680	T1671	punct	", ",recruit
R7490	T11706	T11701	prep	due,be
R7491	T11707	T11706	pcomp	to,due
R7492	T11708	T11706	pobj	variations,due
R7493	T11709	T11708	prep	in,variations
R7494	T11710	T11711	det	the,response
R7495	T11711	T11709	pobj	response,in
R7496	T11712	T11711	prep	of,response
R7497	T11713	T11714	amod	different,types
R7498	T11714	T11712	pobj	types,of
R7499	T11715	T11714	compound	cell,types
R75	T228	T229	nsubj	We,found
R750	T1571	T1569	pobj	pathways,of
R7500	T11716	T11711	prep	to,response
R7501	T11717	T11718	compound	Snail,expression
R7502	T11718	T11716	pobj	expression,to
R7503	T11719	T11701	punct	.,be
R7504	T11721	T11722	advmod	Alternatively,be
R7505	T11723	T11722	punct	", ",be
R7506	T11724	T11725	det	these,differences
R7507	T11725	T11722	nsubj	differences,be
R7508	T11726	T11722	aux	may,be
R7509	T11727	T11722	acomp	relevant,be
R751	T1681	T1682	nmod	E,cadherin
R7510	T11728	T11727	prep	to,relevant
R7511	T11729	T11730	det	the,benefit
R7512	T11730	T11728	pobj	benefit,to
R7513	T11731	T11730	prep	of,benefit
R7514	T11732	T11731	pcomp	using,of
R7515	T11733	T11734	compound	mouse,models
R7516	T11734	T11732	dobj	models,using
R7517	T11735	T11736	aux	to,reveal
R7518	T11736	T11732	advcl	reveal,using
R7519	T11737	T11736	dobj	functions,reveal
R752	T1572	T1571	nummod	two,pathways
R7520	T11738	T11739	neg	not,recapitulated
R7521	T11739	T11737	acl	recapitulated,functions
R7522	T11740	T11739	advmod	always,recapitulated
R7523	T11741	T11739	prep	in,recapitulated
R7524	T11742	T11743	amod	stable,models
R7525	T11743	T11741	pobj	models,in
R7526	T11744	T11745	compound	cell,line
R7527	T11745	T11743	compound	line,models
R7528	T11746	T11722	punct	.,be
R7529	T11748	T11749	amod	Future,studies
R753	T1573	T1571	amod	early,pathways
R7530	T11749	T11750	nsubj	studies,highlight
R7531	T11751	T11750	aux	should,highlight
R7532	T11752	T11753	det	the,reasons
R7533	T11753	T11750	dobj	reasons,highlight
R7534	T11754	T11753	amod	underlying,reasons
R7535	T11755	T11753	prep	for,reasons
R7536	T11756	T11757	det	these,results
R7537	T11757	T11755	pobj	results,for
R7538	T11758	T11757	amod	opposing,results
R7539	T11759	T11750	punct	.,highlight
R754	T1682	T1684	nmod	cadherin,complexes
R7540	T11761	T11762	advcl	Irrespective,stand
R7541	T11763	T11761	prep	of,Irrespective
R7542	T11764	T11765	det	these,differences
R7543	T11765	T11763	pobj	differences,of
R7544	T11766	T11762	punct	", ",stand
R7545	T11767	T11768	poss	our,studies
R7546	T11768	T11762	nsubj	studies,stand
R7547	T11769	T11770	advmod	in,vivo
R7548	T11770	T11768	amod	vivo,studies
R7549	T11771	T11762	aux	do,stand
R755	T1574	T1571	compound	signaling,pathways
R7550	T11772	T11762	neg	not,stand
R7551	T11773	T11762	advmod	alone,stand
R7552	T11774	T11762	punct	", ",stand
R7553	T11775	T11776	mark	as,are
R7554	T11776	T11762	advcl	are,stand
R7555	T11777	T11776	expl	there,are
R7556	T11778	T11779	amod	many,situations
R7557	T11779	T11776	attr	situations,are
R7558	T11780	T11781	prep	in,correlate
R7559	T11781	T11779	relcl	correlate,situations
R756	T1575	T1576	amod	down,regulation
R7560	T11782	T11780	pobj	which,in
R7561	T11783	T11784	det	a,regulation
R7562	T11784	T11781	nsubj	regulation,correlate
R7563	T11785	T11784	amod	down,regulation
R7564	T11786	T11784	punct	-,regulation
R7565	T11787	T11784	prep	in,regulation
R7566	T11788	T11789	compound	AJ,proteins
R7567	T11789	T11787	pobj	proteins,in
R7568	T11790	T11781	prep	with,correlate
R7569	T11791	T11792	amod	enhanced,proliferation
R757	T1683	T1682	punct	-,cadherin
R7570	T11792	T11790	pobj	proliferation,with
R7571	T11793	T11762	punct	.,stand
R7572	T11795	T11796	prep	In,implicated
R7573	T11797	T11795	pobj	fact,In
R7574	T11798	T11796	punct	", ",implicated
R7575	T11799	T11800	det	a,myriad
R7576	T11800	T11796	nsubjpass	myriad,implicated
R7577	T11801	T11800	prep	of,myriad
R7578	T11802	T11803	amod	diverse,mechanisms
R7579	T11803	T11801	pobj	mechanisms,of
R758	T1576	T1565	dobj	regulation,include
R7580	T11804	T11796	aux	have,implicated
R7581	T11805	T11796	auxpass	been,implicated
R7582	T11806	T11796	prep	in,implicated
R7583	T11807	T11806	pcomp	activating,in
R7584	T11808	T11809	amod	epithelial,proliferation
R7585	T11809	T11807	dobj	proliferation,activating
R7586	T11810	T11807	prep	upon,activating
R7587	T11811	T11812	amod	down,regulation
R7588	T11812	T11810	pobj	regulation,upon
R7589	T11813	T11812	punct	-,regulation
R759	T1577	T1576	punct	-,regulation
R7590	T11814	T11812	prep	of,regulation
R7591	T11815	T11816	compound	AJ,proteins
R7592	T11816	T11814	pobj	proteins,of
R7593	T11817	T11818	punct	[,48
R7594	T11818	T11796	parataxis	48,implicated
R7595	T11819	T11818	nummod	7,48
R7596	T11820	T11818	punct	",",48
R7597	T11821	T11818	nummod	23,48
R7598	T11822	T11818	punct	",",48
R7599	T11823	T11818	nummod	24,48
R76	T230	T231	mark	that,is
R760	T1578	T1576	prep	of,regulation
R7600	T11824	T11818	punct	",",48
R7601	T11825	T11818	punct	],48
R7602	T11826	T11796	punct	.,implicated
R7603	T11828	T11829	csubj	Sifting,is
R7604	T11830	T11828	prep	through,Sifting
R7605	T11831	T11832	det	these,pathways
R7606	T11832	T11830	pobj	pathways,through
R7607	T11833	T11832	amod	converging,pathways
R7608	T11834	T11829	acomp	likely,is
R7609	T11835	T11836	aux	to,be
R761	T1684	T1671	nsubj	complexes,recruit
R7610	T11836	T11834	xcomp	be,likely
R7611	T11837	T11838	det	a,process
R7612	T11838	T11836	attr	process,be
R7613	T11839	T11838	amod	difficult,process
R7614	T11840	T11839	cc	and,difficult
R7615	T11841	T11839	conj	painstaking,difficult
R7616	T11842	T11829	punct	.,is
R7617	T11844	T11845	nsubj	This,said
R7618	T11845	T11846	advcl	said,begin
R7619	T11847	T11846	punct	", ",begin
R762	T1579	T1580	det	the,gene
R7620	T11848	T11846	prep	by,begin
R7621	T11849	T11848	pcomp	identifying,by
R7622	T11850	T11851	det	the,status
R7623	T11851	T11849	dobj	status,identifying
R7624	T11852	T11851	prep	of,status
R7625	T11853	T11854	amod	different,players
R7626	T11854	T11852	pobj	players,of
R7627	T11855	T11854	acl	involved,players
R7628	T11856	T11849	prep	in,identifying
R7629	T11857	T11858	amod	specific,types
R763	T1580	T1578	pobj	gene,of
R7630	T11858	T11856	pobj	types,in
R7631	T11859	T11858	compound	cell,types
R7632	T11860	T11856	cc	and,in
R7633	T11861	T11856	conj	at,in
R7634	T11862	T11863	amod	specific,stages
R7635	T11863	T11861	pobj	stages,at
R7636	T11864	T11863	prep	in,stages
R7637	T11865	T11864	pobj	development,in
R7638	T11866	T11846	punct	", ",begin
R7639	T11867	T11868	poss	our,understanding
R764	T1685	T1682	punct	-,cadherin
R7640	T11868	T11846	nsubj	understanding,begin
R7641	T11869	T11868	amod	mechanistic,understanding
R7642	T11870	T11868	prep	of,understanding
R7643	T11871	T11872	advmod	how,linked
R7644	T11872	T11870	pcomp	linked,of
R7645	T11873	T11874	amod	intercellular,remodeling
R7646	T11874	T11872	nsubjpass	remodeling,linked
R7647	T11875	T11872	auxpass	is,linked
R7648	T11876	T11872	prep	to,linked
R7649	T11877	T11876	pobj	proliferation,to
R765	T1581	T1580	acl	encoding,gene
R7650	T11878	T11872	prep	in,linked
R7651	T11879	T11880	amod	epithelial,morphogenesis
R7652	T11880	T11878	pobj	morphogenesis,in
R7653	T11881	T11846	aux	should,begin
R7654	T11882	T11883	aux	to,surface
R7655	T11883	T11846	xcomp	surface,begin
R7656	T11884	T11883	prep	in,surface
R7657	T11885	T11886	det	the,future
R7658	T11886	T11884	pobj	future,in
R7659	T11887	T11846	punct	.,begin
R766	T1582	T1583	compound	E,cadherin
R7660	T11889	T11890	csubj	Elucidating,is
R7661	T11891	T11892	det	the,mechanisms
R7662	T11892	T11889	dobj	mechanisms,Elucidating
R7663	T11893	T11892	amod	molecular,mechanisms
R7664	T11894	T11895	prep	through,converge
R7665	T11895	T11892	relcl	converge,mechanisms
R7666	T11896	T11894	pobj	which,through
R7667	T11897	T11898	det	these,networks
R7668	T11898	T11895	nsubj	networks,converge
R7669	T11899	T11890	advmod	also,is
R767	T1583	T1581	dobj	cadherin,encoding
R7670	T11900	T11901	det	a,prerequisite
R7671	T11901	T11890	attr	prerequisite,is
R7672	T11902	T11901	prep	for,prerequisite
R7673	T11903	T11902	pcomp	understanding,for
R7674	T11904	T11905	advmod	how,go
R7675	T11905	T11903	ccomp	go,understanding
R7676	T11906	T11907	det	these,processes
R7677	T11907	T11905	nsubj	processes,go
R7678	T11908	T11905	advmod	awry,go
R7679	T11909	T11905	prep	during,go
R768	T1686	T1687	compound	β,catenin
R7680	T11910	T11909	pobj	tumorigenesis,during
R7681	T11911	T11890	punct	.,is
R7682	T12023	T12024	amod	Primary,antibodies
R7683	T12024	T12025	nsubj	antibodies,were
R7684	T12026	T12024	acl	used,antibodies
R7685	T12027	T12025	prep	against,were
R7686	T12028	T12027	punct	: ,against
R7687	T12029	T12030	compound	E,cadherin
R7688	T12030	T12027	pobj	cadherin,against
R7689	T12031	T12030	punct	-,cadherin
R769	T1584	T1583	punct	-,cadherin
R7690	T12032	T12033	punct	(,Takeichi
R7691	T12033	T12030	parataxis	Takeichi,cadherin
R7692	T12034	T12033	compound	M.,Takeichi
R7693	T12035	T12033	punct	", ",Takeichi
R7694	T12036	T12037	compound	Kyoto,University
R7695	T12037	T12033	npadvmod	University,Takeichi
R7696	T12038	T12033	punct	", ",Takeichi
R7697	T12039	T12033	npadvmod	Japan,Takeichi
R7698	T12040	T12033	punct	),Takeichi
R7699	T12041	T12030	punct	;,cadherin
R77	T231	T229	ccomp	is,found
R770	T1585	T1583	punct	", ",cadherin
R7700	T12042	T12043	compound	α,catenin
R7701	T12043	T12030	conj	catenin,cadherin
R7702	T12044	T12043	punct	-,catenin
R7703	T12045	T12043	punct	", ",catenin
R7704	T12046	T12047	compound	β,catenin
R7705	T12047	T12043	appos	catenin,catenin
R7706	T12048	T12047	punct	-,catenin
R7707	T12049	T12043	punct	", ",catenin
R7708	T12050	T12043	appos	pMAPK,catenin
R7709	T12051	T12043	punct	", ",catenin
R771	T1586	T1587	det	the,cadherin
R7710	T12052	T12043	appos	tubulin,catenin
R7711	T12053	T12054	punct	(,Sigma
R7712	T12054	T12043	parataxis	Sigma,catenin
R7713	T12055	T12054	punct	", ",Sigma
R7714	T12056	T12057	compound	St.,Louis
R7715	T12057	T12054	npadvmod	Louis,Sigma
R7716	T12058	T12054	punct	", ",Sigma
R7717	T12059	T12054	npadvmod	Missouri,Sigma
R7718	T12060	T12054	punct	", ",Sigma
R7719	T12061	T12062	compound	United,States
R772	T1687	T1682	appos	catenin,cadherin
R7720	T12062	T12054	npadvmod	States,Sigma
R7721	T12063	T12054	punct	),Sigma
R7722	T12064	T12043	punct	", ",catenin
R7723	T12065	T12043	conj	Ajuba,catenin
R7724	T12066	T12067	punct	(,Longmore
R7725	T12067	T12065	parataxis	Longmore,Ajuba
R7726	T12068	T12067	compound	G.,Longmore
R7727	T12069	T12067	punct	", ",Longmore
R7728	T12070	T12071	compound	Washington,University
R7729	T12071	T12067	npadvmod	University,Longmore
R773	T1587	T1583	appos	cadherin,cadherin
R7730	T12072	T12067	punct	", ",Longmore
R7731	T12073	T12074	compound	St.,Louis
R7732	T12074	T12067	npadvmod	Louis,Longmore
R7733	T12075	T12067	punct	", ",Longmore
R7734	T12076	T12067	npadvmod	Missouri,Longmore
R7735	T12077	T12067	punct	", ",Longmore
R7736	T12078	T12079	compound	United,States
R7737	T12079	T12067	npadvmod	States,Longmore
R7738	T12080	T12067	punct	),Longmore
R7739	T12081	T12065	punct	;,Ajuba
R774	T1588	T1587	amod	prototypical,cadherin
R7740	T12082	T12083	compound	β4,integrin
R7741	T12083	T12065	conj	integrin,Ajuba
R7742	T12084	T12083	punct	/,integrin
R7743	T12085	T12083	appos	CD104,integrin
R7744	T12086	T12087	punct	(,Pharmingen
R7745	T12087	T12083	parataxis	Pharmingen,integrin
R7746	T12088	T12087	compound	BD,Pharmingen
R7747	T12089	T12087	punct	", ",Pharmingen
R7748	T12090	T12091	compound	San,Diego
R7749	T12091	T12087	npadvmod	Diego,Pharmingen
R775	T1688	T1687	punct	-,catenin
R7750	T12092	T12087	punct	", ",Pharmingen
R7751	T12093	T12087	npadvmod	California,Pharmingen
R7752	T12094	T12087	punct	", ",Pharmingen
R7753	T12095	T12096	compound	United,States
R7754	T12096	T12087	npadvmod	States,Pharmingen
R7755	T12097	T12087	punct	),Pharmingen
R7756	T12098	T12083	punct	", ",integrin
R7757	T12099	T12083	conj	laminin,integrin
R7758	T12100	T12099	nummod	5,laminin
R7759	T12101	T12102	punct	(,Burgeson
R776	T1589	T1587	amod	epithelial,cadherin
R7760	T12102	T12099	parataxis	Burgeson,laminin
R7761	T12103	T12102	compound	R.,Burgeson
R7762	T12104	T12102	punct	", ",Burgeson
R7763	T12105	T12106	compound	Harvard,University
R7764	T12106	T12102	npadvmod	University,Burgeson
R7765	T12107	T12102	punct	", ",Burgeson
R7766	T12108	T12102	npadvmod	Cambridge,Burgeson
R7767	T12109	T12102	punct	", ",Burgeson
R7768	T12110	T12102	npadvmod	Massachusetts,Burgeson
R7769	T12111	T12102	punct	", ",Burgeson
R777	T1590	T1591	dep	that,forms
R7770	T12112	T12113	compound	United,States
R7771	T12113	T12102	npadvmod	States,Burgeson
R7772	T12114	T12102	punct	),Burgeson
R7773	T12115	T12099	punct	", ",laminin
R7774	T12116	T12099	conj	K5,laminin
R7775	T12117	T12116	punct	", ",K5
R7776	T12118	T12116	conj	K1,K5
R7777	T12119	T12118	punct	", ",K1
R7778	T12120	T12118	conj	loricrin,K1
R7779	T12121	T12122	punct	(,Lab
R778	T1591	T1587	relcl	forms,cadherin
R7780	T12122	T12120	parataxis	Lab,loricrin
R7781	T12123	T12122	compound	Fuchs,Lab
R7782	T12124	T12122	punct	),Lab
R7783	T12125	T12120	punct	", ",loricrin
R7784	T12126	T12120	conj	involucrin,loricrin
R7785	T12127	T12126	punct	", ",involucrin
R7786	T12128	T12126	conj	fillagrin,involucrin
R7787	T12129	T12130	punct	(,Covance
R7788	T12130	T12128	parataxis	Covance,fillagrin
R7789	T12131	T12130	punct	", ",Covance
R779	T1689	T1690	compound	α,catenin
R7790	T12132	T12130	npadvmod	Berkeley,Covance
R7791	T12133	T12130	punct	", ",Covance
R7792	T12134	T12130	npadvmod	California,Covance
R7793	T12135	T12130	punct	", ",Covance
R7794	T12136	T12137	compound	United,States
R7795	T12137	T12130	npadvmod	States,Covance
R7796	T12138	T12130	punct	),Covance
R7797	T12139	T12128	punct	", ",fillagrin
R7798	T12140	T12128	conj	MAPK,fillagrin
R7799	T12141	T12140	punct	", ",MAPK
R78	T232	T231	csubj	transforming,is
R780	T1592	T1593	det	the,core
R7800	T12142	T12140	conj	pSMAD2,MAPK
R7801	T12143	T12144	punct	(,Signaling
R7802	T12144	T12142	parataxis	Signaling,pSMAD2
R7803	T12145	T12144	compound	Cell,Signaling
R7804	T12146	T12144	punct	", ",Signaling
R7805	T12147	T12144	npadvmod	Beverly,Signaling
R7806	T12148	T12144	punct	", ",Signaling
R7807	T12149	T12144	npadvmod	Massachusetts,Signaling
R7808	T12150	T12144	punct	", ",Signaling
R7809	T12151	T12152	compound	United,States
R781	T1593	T1591	dobj	core,forms
R7810	T12152	T12144	npadvmod	States,Signaling
R7811	T12153	T12144	punct	),Signaling
R7812	T12154	T12142	punct	;,pSMAD2
R7813	T12155	T12142	conj	Grb,pSMAD2
R7814	T12156	T12155	punct	-,Grb
R7815	T12157	T12155	nummod	2,Grb
R7816	T12158	T12159	punct	(,Biotech
R7817	T12159	T12155	parataxis	Biotech,Grb
R7818	T12160	T12161	compound	Santa,Cruz
R7819	T12161	T12159	compound	Cruz,Biotech
R782	T1690	T1671	dobj	catenin,recruit
R7820	T12162	T12159	punct	", ",Biotech
R7821	T12163	T12164	compound	Santa,Cruz
R7822	T12164	T12159	npadvmod	Cruz,Biotech
R7823	T12165	T12159	punct	", ",Biotech
R7824	T12166	T12159	npadvmod	California,Biotech
R7825	T12167	T12159	punct	", ",Biotech
R7826	T12168	T12169	compound	United,States
R7827	T12169	T12159	npadvmod	States,Biotech
R7828	T12170	T12159	punct	),Biotech
R7829	T12171	T12155	punct	;,Grb
R783	T1594	T1593	amod	transmembrane,core
R7830	T12172	T12173	compound	P,cadherin
R7831	T12173	T12155	conj	cadherin,Grb
R7832	T12174	T12173	punct	-,cadherin
R7833	T12175	T12176	punct	(,Laboratories
R7834	T12176	T12173	parataxis	Laboratories,cadherin
R7835	T12177	T12176	compound	Zymed,Laboratories
R7836	T12178	T12176	punct	", ",Laboratories
R7837	T12179	T12180	amod	South,Francisco
R7838	T12180	T12176	npadvmod	Francisco,Laboratories
R7839	T12181	T12180	compound	San,Francisco
R784	T1595	T1593	prep	of,core
R7840	T12182	T12176	punct	", ",Laboratories
R7841	T12183	T12176	npadvmod	California,Laboratories
R7842	T12184	T12176	punct	", ",Laboratories
R7843	T12185	T12186	compound	United,States
R7844	T12186	T12176	npadvmod	States,Laboratories
R7845	T12187	T12176	punct	),Laboratories
R7846	T12188	T12173	punct	;,cadherin
R7847	T12189	T12173	conj	HA,cadherin
R7848	T12190	T12191	punct	(,Biochemicals
R7849	T12191	T12189	parataxis	Biochemicals,HA
R785	T1596	T1597	amod	intercellular,junctions
R7850	T12192	T12191	compound	Roche,Biochemicals
R7851	T12193	T12191	punct	),Biochemicals
R7852	T12194	T12189	punct	", ",HA
R7853	T12195	T12189	conj	vimentin,HA
R7854	T12196	T12197	punct	(,Chemicon
R7855	T12197	T12195	parataxis	Chemicon,vimentin
R7856	T12198	T12197	punct	", ",Chemicon
R7857	T12199	T12197	npadvmod	Temecula,Chemicon
R7858	T12200	T12197	punct	", ",Chemicon
R7859	T12201	T12197	npadvmod	California,Chemicon
R786	T1691	T1690	punct	-,catenin
R7860	T12202	T12197	punct	", ",Chemicon
R7861	T12203	T12204	compound	United,States
R7862	T12204	T12197	npadvmod	States,Chemicon
R7863	T12205	T12197	punct	),Chemicon
R7864	T12206	T12195	punct	", ",vimentin
R7865	T12207	T12195	conj	Ki67,vimentin
R7866	T12208	T12209	punct	(,Castra
R7867	T12209	T12207	parataxis	Castra,Ki67
R7868	T12210	T12209	compound	Novo,Castra
R7869	T12211	T12209	punct	", ",Castra
R787	T1597	T1595	pobj	junctions,of
R7870	T12212	T12209	npadvmod	Newcastle,Castra
R7871	T12213	T12212	prep	Upon,Newcastle
R7872	T12214	T12213	pobj	Tyne,Upon
R7873	T12215	T12209	punct	", ",Castra
R7874	T12216	T12217	compound	United,Kingdom
R7875	T12217	T12209	npadvmod	Kingdom,Castra
R7876	T12218	T12209	punct	),Castra
R7877	T12219	T12207	punct	", ",Ki67
R7878	T12220	T12207	conj	keratin,Ki67
R7879	T12221	T12220	nummod	6,keratin
R788	T1598	T1597	compound	adherens,junctions
R7880	T12222	T12223	punct	(,Coulombe
R7881	T12223	T12220	parataxis	Coulombe,keratin
R7882	T12224	T12223	compound	P.,Coulombe
R7883	T12225	T12223	punct	", ",Coulombe
R7884	T12226	T12227	compound	John,Hopkins
R7885	T12227	T12228	compound	Hopkins,University
R7886	T12228	T12223	npadvmod	University,Coulombe
R7887	T12229	T12223	punct	", ",Coulombe
R7888	T12230	T12223	npadvmod	Baltimore,Coulombe
R7889	T12231	T12223	punct	", ",Coulombe
R789	T1599	T1597	punct	(,junctions
R7890	T12232	T12223	npadvmod	Maryland,Coulombe
R7891	T12233	T12223	punct	", ",Coulombe
R7892	T12234	T12235	compound	United,States
R7893	T12235	T12223	npadvmod	States,Coulombe
R7894	T12236	T12223	punct	),Coulombe
R7895	T12237	T12220	punct	", ",keratin
R7896	T12238	T12239	compound	cyclin,D
R7897	T12239	T12220	conj	D,keratin
R7898	T12240	T12241	punct	(,Oncogene
R7899	T12241	T12239	parataxis	Oncogene,D
R79	T233	T234	compound	growth,β2
R790	T1692	T1690	punct	", ",catenin
R7900	T12242	T12241	punct	", ",Oncogene
R7901	T12243	T12244	compound	San,Diego
R7902	T12244	T12241	npadvmod	Diego,Oncogene
R7903	T12245	T12241	punct	", ",Oncogene
R7904	T12246	T12241	npadvmod	California,Oncogene
R7905	T12247	T12241	punct	", ",Oncogene
R7906	T12248	T12249	compound	United,States
R7907	T12249	T12241	npadvmod	States,Oncogene
R7908	T12250	T12241	punct	),Oncogene
R7909	T12251	T12239	punct	", ",D
R791	T1600	T1597	appos	AJs,junctions
R7910	T12252	T12239	cc	and,D
R7911	T12253	T12254	compound	TGF,β2
R7912	T12254	T12239	conj	β2,D
R7913	T12255	T12254	punct	-,β2
R7914	T12256	T12257	punct	(,Gold
R7915	T12257	T12254	parataxis	Gold,β2
R7916	T12258	T12257	compound	L.,Gold
R7917	T12259	T12257	punct	", ",Gold
R7918	T12260	T12261	compound	New,York
R7919	T12261	T12262	compound	York,University
R792	T1601	T1565	punct	),include
R7920	T12262	T12257	npadvmod	University,Gold
R7921	T12263	T12257	punct	", ",Gold
R7922	T12264	T12265	compound	New,York
R7923	T12265	T12257	npadvmod	York,Gold
R7924	T12266	T12257	punct	", ",Gold
R7925	T12267	T12268	compound	New,York
R7926	T12268	T12257	npadvmod	York,Gold
R7927	T12269	T12257	punct	", ",Gold
R7928	T12270	T12271	compound	United,States
R7929	T12271	T12257	npadvmod	States,Gold
R793	T1602	T1603	punct	[,5
R7930	T12272	T12257	punct	),Gold
R7931	T12273	T12025	punct	.,were
R7932	T12275	T12276	npadvmod	FITC,conjugated
R7933	T12276	T12286	amod	conjugated,antibodies
R7934	T12277	T12275	punct	-,FITC
R7935	T12278	T12275	punct	", ",FITC
R7936	T12279	T12280	compound	Texas,Red
R7937	T12280	T12275	conj	Red,FITC
R7938	T12281	T12280	punct	-,Red
R7939	T12282	T12280	punct	", ",Red
R794	T1603	T1565	parataxis	5,include
R7940	T12283	T12280	cc	or,Red
R7941	T12284	T12280	conj	HRP,Red
R7942	T12285	T12276	punct	-,conjugated
R7943	T12286	T12288	nsubj	antibodies,were
R7944	T12287	T12286	amod	secondary,antibodies
R7945	T12289	T12288	prep	from,were
R7946	T12290	T12291	compound	Jackson,ImmunoResearch
R7947	T12291	T12289	pobj	ImmunoResearch,from
R7948	T12292	T12293	punct	(,Grove
R7949	T12293	T12291	parataxis	Grove,ImmunoResearch
R795	T1604	T1603	punct	],5
R7950	T12294	T12293	compound	West,Grove
R7951	T12295	T12293	punct	", ",Grove
R7952	T12296	T12293	npadvmod	Pennsylvania,Grove
R7953	T12297	T12293	punct	", ",Grove
R7954	T12298	T12299	compound	United,States
R7955	T12299	T12293	npadvmod	States,Grove
R7956	T12300	T12293	punct	),Grove
R7957	T12301	T12288	punct	.,were
R7958	T12303	T12304	amod	Biotinylated,antibodies
R7959	T12304	T12306	nsubj	antibodies,were
R796	T1693	T1694	dep	which,coordinates
R7960	T12305	T12304	amod	secondary,antibodies
R7961	T12307	T12306	prep	from,were
R7962	T12308	T12309	compound	Vector,Labs
R7963	T12309	T12307	pobj	Labs,from
R7964	T12310	T12311	punct	(,Burlingame
R7965	T12311	T12309	parataxis	Burlingame,Labs
R7966	T12312	T12311	punct	", ",Burlingame
R7967	T12313	T12311	npadvmod	California,Burlingame
R7968	T12314	T12311	punct	", ",Burlingame
R7969	T12315	T12316	compound	United,States
R797	T1605	T1565	punct	.,include
R7970	T12316	T12311	npadvmod	States,Burlingame
R7971	T12317	T12311	punct	),Burlingame
R7972	T12318	T12306	punct	.,were
R7973	T12320	T12321	nsubj	Dilutions,were
R7974	T12322	T12321	prep	according,were
R7975	T12323	T12322	prep	to,according
R7976	T12324	T12325	det	the,manufacturer
R7977	T12325	T12326	poss	manufacturer,recommendation
R7978	T12326	T12323	pobj	recommendation,to
R7979	T12327	T12325	case	's,manufacturer
R798	T1607	T1608	nsubj	We,showed
R7980	T12328	T12321	punct	.,were
R7981	T12330	T12331	det	The,antibody
R7982	T12331	T12333	nsubjpass	antibody,generated
R7983	T12332	T12331	compound	Snail,antibody
R7984	T12334	T12333	auxpass	was,generated
R7985	T12335	T12333	prep	in,generated
R7986	T12336	T12337	compound	Guinea,pigs
R7987	T12337	T12335	pobj	pigs,in
R7988	T12338	T12333	prep	by,generated
R7989	T12339	T12338	pcomp	inoculating,by
R799	T1694	T1690	relcl	coordinates,catenin
R7990	T12340	T12339	dobj	them,inoculating
R7991	T12341	T12339	prep	with,inoculating
R7992	T12342	T12343	det	the,sequence
R7993	T12343	T12341	pobj	sequence,with
R7994	T12344	T12345	npadvmod	N,terminal
R7995	T12345	T12343	amod	terminal,sequence
R7996	T12346	T12345	punct	-,terminal
R7997	T12347	T12343	prep	of,sequence
R7998	T12348	T12349	amod	murine,Snail
R7999	T12349	T12347	pobj	Snail,of
R8	T155	T152	conj	Snail,β2
R80	T234	T236	compound	β2,signaling
R800	T1609	T1608	advmod	subsequently,showed
R8000	T12350	T12343	acl	fused,sequence
R8001	T12351	T12350	prep	to,fused
R8002	T12352	T12351	pobj	GST,to
R8003	T12353	T12354	punct	(,Covance
R8004	T12354	T12352	parataxis	Covance,GST
R8005	T12355	T12354	punct	", ",Covance
R8006	T12356	T12354	npadvmod	Princeton,Covance
R8007	T12357	T12354	punct	", ",Covance
R8008	T12358	T12359	compound	New,Jersey
R8009	T12359	T12354	npadvmod	Jersey,Covance
R801	T1695	T1694	prep	in,coordinates
R8010	T12360	T12354	punct	", ",Covance
R8011	T12361	T12362	compound	United,States
R8012	T12362	T12354	npadvmod	States,Covance
R8013	T12363	T12354	punct	),Covance
R8014	T12364	T12333	punct	.,generated
R8015	T12366	T12367	amod	Recombinant,β2
R8016	T12367	T12371	nsubjpass	β2,purchased
R8017	T12368	T12367	amod	human,β2
R8018	T12369	T12367	compound	TGF,β2
R8019	T12370	T12367	punct	-,β2
R802	T1610	T1611	mark	that,blocked
R8020	T12372	T12371	auxpass	was,purchased
R8021	T12373	T12371	prep	from,purchased
R8022	T12374	T12373	pobj	R,from
R8023	T12375	T12374	cc	&,R
R8024	T12376	T12374	conj	D,R
R8025	T12377	T12378	punct	(,Minneapolis
R8026	T12378	T12376	parataxis	Minneapolis,D
R8027	T12379	T12378	punct	", ",Minneapolis
R8028	T12380	T12378	npadvmod	Minnesota,Minneapolis
R8029	T12381	T12378	punct	", ",Minneapolis
R803	T1696	T1695	pobj	turn,in
R8030	T12382	T12383	compound	United,States
R8031	T12383	T12378	npadvmod	States,Minneapolis
R8032	T12384	T12378	punct	),Minneapolis
R8033	T12385	T12371	punct	.,purchased
R8034	T12387	T12388	npadvmod	Heat,inactivated
R8035	T12388	T12389	amod	inactivated,β2
R8036	T12389	T12392	nsubjpass	β2,generated
R8037	T12390	T12389	compound	TGF,β2
R8038	T12391	T12389	punct	-,β2
R8039	T12393	T12392	auxpass	was,generated
R804	T1611	T1608	ccomp	blocked,showed
R8040	T12394	T12392	prep	by,generated
R8041	T12395	T12394	pcomp	heating,by
R8042	T12396	T12397	det	the,protein
R8043	T12397	T12395	dobj	protein,heating
R8044	T12398	T12397	amod	recombinant,protein
R8045	T12399	T12395	prep	at,heating
R8046	T12400	T12401	nummod	100,°C
R8047	T12401	T12399	pobj	°C,at
R8048	T12402	T12395	prep	for,heating
R8049	T12403	T12404	nummod	10,min
R805	T1697	T1698	det	the,dynamics
R8050	T12404	T12402	pobj	min,for
R8051	T12405	T12392	punct	.,generated
R8052	T12466	T12467	det	The,mouse
R8053	T12467	T12472	nsubjpass	mouse,generated
R8054	T12468	T12469	compound	K14,Snail
R8055	T12469	T12467	compound	Snail,mouse
R8056	T12470	T12469	punct	-,Snail
R8057	T12471	T12467	compound	Tg,mouse
R8058	T12473	T12472	auxpass	was,generated
R8059	T12474	T12472	prep	by,generated
R806	T1612	T1613	advmod	when,elevated
R8060	T12475	T12474	pcomp	digesting,by
R8061	T12476	T12477	det	the,plasmid
R8062	T12477	T12475	dobj	plasmid,digesting
R8063	T12478	T12479	compound	pcDNA3,mm
R8064	T12479	T12477	compound	mm,plasmid
R8065	T12480	T12479	punct	-,mm
R8066	T12481	T12482	compound	Snail,HA
R8067	T12482	T12477	compound	HA,plasmid
R8068	T12483	T12482	punct	-,HA
R8069	T12484	T12485	punct	(,Herreros
R807	T1613	T1611	advcl	elevated,blocked
R8070	T12485	T12477	parataxis	Herreros,plasmid
R8071	T12486	T12485	compound	G.,Herreros
R8072	T12487	T12485	compound	de,Herreros
R8073	T12488	T12485	punct	", ",Herreros
R8074	T12489	T12490	compound	Universitat,Pompeu
R8075	T12490	T12485	npadvmod	Pompeu,Herreros
R8076	T12491	T12485	punct	", ",Herreros
R8077	T12492	T12485	npadvmod	Fabra,Herreros
R8078	T12493	T12485	punct	", ",Herreros
R8079	T12494	T12485	npadvmod	Barcelona,Herreros
R808	T1698	T1694	dobj	dynamics,coordinates
R8080	T12495	T12485	punct	", ",Herreros
R8081	T12496	T12485	npadvmod	Spain,Herreros
R8082	T12497	T12485	punct	),Herreros
R8083	T12498	T12475	prep	with,digesting
R8084	T12499	T12498	pobj	BamHI,with
R8085	T12500	T12499	cc	and,BamHI
R8086	T12501	T12499	conj	NotI,BamHI
R8087	T12502	T12472	cc	and,generated
R8088	T12503	T12472	conj	subcloned,generated
R8089	T12504	T12503	prep	into,subcloned
R809	T1614	T1615	compound	E,cadherin
R8090	T12505	T12506	det	the,vector
R8091	T12506	T12504	pobj	vector,into
R8092	T12507	T12506	compound	K14,vector
R8093	T12508	T12509	punct	[,49
R8094	T12509	T12503	parataxis	49,subcloned
R8095	T12510	T12509	punct	],49
R8096	T12511	T12472	punct	.,generated
R8097	T12513	T12514	det	The,construct
R8098	T12514	T12516	nsubjpass	construct,injected
R8099	T12515	T12514	amod	linearized,construct
R81	T235	T234	compound	factor,β2
R810	T1615	T1613	nsubjpass	cadherin,elevated
R8100	T12517	T12516	auxpass	was,injected
R8101	T12518	T12516	prep	into,injected
R8102	T12519	T12520	det	the,nucleus
R8103	T12520	T12518	pobj	nucleus,into
R8104	T12521	T12520	prep	of,nucleus
R8105	T12522	T12521	pobj	embryos,of
R8106	T12523	T12522	prep	from,embryos
R8107	T12524	T12525	compound	CD1,mice
R8108	T12525	T12523	pobj	mice,from
R8109	T12526	T12516	punct	.,injected
R811	T1616	T1615	punct	-,cadherin
R8110	T12528	T12529	det	The,mouse
R8111	T12529	T12535	nsubjpass	mouse,reported
R8112	T12530	T12531	nmod	K14,Smad
R8113	T12531	T12529	nmod	Smad,mouse
R8114	T12532	T12531	punct	-,Smad
R8115	T12533	T12534	nummod	2,Tg
R8116	T12534	T12529	compound	Tg,mouse
R8117	T12536	T12535	auxpass	was,reported
R8118	T12537	T12535	prep	in,reported
R8119	T12538	T12537	pobj	Ito,in
R812	T1699	T1698	amod	associated,dynamics
R8120	T12539	T12540	advmod	et,al.
R8121	T12540	T12538	advmod	al.,Ito
R8122	T12541	T12538	punct	", ",Ito
R8123	T12542	T12538	npadvmod	2001,Ito
R8124	T12543	T12535	punct	.,reported
R8125	T12545	T12546	det	The,mouse
R8126	T12546	T12554	nsubjpass	mouse,described
R8127	T12547	T12548	nmod	TGF,β2
R8128	T12548	T12546	nmod	β2,mouse
R8129	T12549	T12548	punct	-,β2
R813	T1617	T1613	auxpass	is,elevated
R8130	T12550	T12548	appos	knockout,β2
R8131	T12551	T12550	punct	(,knockout
R8132	T12552	T12550	appos	KO,knockout
R8133	T12553	T12546	punct	),mouse
R8134	T12555	T12554	auxpass	was,described
R8135	T12556	T12554	prep	in,described
R8136	T12557	T12558	punct	[,34
R8137	T12558	T12556	pobj	34,in
R8138	T12559	T12554	punct	],described
R8139	T12560	T12554	punct	.,described
R814	T1618	T1613	advmod	transgenically,elevated
R8140	T12562	T12563	det	The,mouse
R8141	T12563	T12566	nsubjpass	mouse,reported
R8142	T12564	T12565	compound	shh,KO
R8143	T12565	T12563	compound	KO,mouse
R8144	T12567	T12568	punct	[,38
R8145	T12568	T12563	parataxis	38,mouse
R8146	T12569	T12568	punct	],38
R8147	T12570	T12563	cc	and,mouse
R8148	T12571	T12572	compound	TOPGal,mouse
R8149	T12572	T12563	conj	mouse,mouse
R815	T1700	T1698	compound	actin,dynamics
R8150	T12573	T12574	punct	[,20
R8151	T12574	T12572	parataxis	20,mouse
R8152	T12575	T12574	punct	],20
R8153	T12576	T12566	aux	have,reported
R8154	T12577	T12566	advmod	previously,reported
R8155	T12578	T12566	auxpass	been,reported
R8156	T12579	T12566	punct	.,reported
R8157	T12687	T12688	compound	Western,blot
R8158	T12689	T12688	cc	and,blot
R8159	T12690	T12688	conj	immunoprecipitation,blot
R816	T1619	T1613	prep	in,elevated
R8160	T12692	T12693	compound	Protein,extracts
R8161	T12693	T12694	nsubjpass	extracts,generated
R8162	T12695	T12693	prep	from,extracts
R8163	T12696	T12697	amod	primary,keratinocytes
R8164	T12697	T12695	pobj	keratinocytes,from
R8165	T12698	T12694	auxpass	were,generated
R8166	T12699	T12700	preconj	either,by
R8167	T12700	T12694	prep	by,generated
R8168	T12701	T12700	pcomp	lysing,by
R8169	T12702	T12701	dobj	cells,lysing
R817	T1620	T1621	compound	mouse,skin
R8170	T12703	T12701	prep	in,lysing
R8171	T12704	T12705	compound	lysis,buffer
R8172	T12705	T12703	pobj	buffer,in
R8173	T12706	T12707	punct	(,NP
R8174	T12707	T12705	parataxis	NP,buffer
R8175	T12708	T12709	nummod	1,%
R8176	T12709	T12707	compound	%,NP
R8177	T12710	T12707	punct	-,NP
R8178	T12711	T12707	nummod	40,NP
R8179	T12712	T12707	punct	", ",NP
R818	T1621	T1619	pobj	skin,in
R8180	T12713	T12714	nummod	1,%
R8181	T12714	T12715	compound	%,deoxycholate
R8182	T12715	T12707	conj	deoxycholate,NP
R8183	T12716	T12715	compound	sodium,deoxycholate
R8184	T12717	T12715	punct	", ",deoxycholate
R8185	T12718	T12719	nummod	20,mM
R8186	T12719	T12720	compound	mM,Cl
R8187	T12720	T12715	conj	Cl,deoxycholate
R8188	T12721	T12720	compound	Tris,Cl
R8189	T12722	T12720	punct	-,Cl
R819	T1701	T1698	compound	polymerization,dynamics
R8190	T12723	T12720	punct	[,Cl
R8191	T12724	T12720	npadvmod	pH,Cl
R8192	T12725	T12724	nummod	7.4,pH
R8193	T12726	T12720	punct	],Cl
R8194	T12727	T12720	punct	", ",Cl
R8195	T12728	T12729	nummod	140,mM
R8196	T12729	T12730	compound	mM,NaCl
R8197	T12730	T12720	conj	NaCl,Cl
R8198	T12731	T12730	acl	containing,NaCl
R8199	T12732	T12733	nummod	1,mM
R82	T236	T232	dobj	signaling,transforming
R820	T1622	T1611	punct	", ",blocked
R8200	T12733	T12734	compound	mM,vanadate
R8201	T12734	T12731	dobj	vanadate,containing
R8202	T12735	T12734	compound	sodium,vanadate
R8203	T12736	T12730	punct	", ",NaCl
R8204	T12737	T12738	nummod	2,mM
R8205	T12738	T12739	compound	mM,fluoride
R8206	T12739	T12730	conj	fluoride,NaCl
R8207	T12740	T12739	compound	phenylmethylsulfonyl,fluoride
R8208	T12741	T12739	punct	", ",fluoride
R8209	T12742	T12739	cc	and,fluoride
R821	T1623	T1624	compound	hair,follicle
R8210	T12743	T12744	compound	protease,inhibitors
R8211	T12744	T12739	conj	inhibitors,fluoride
R8212	T12745	T12707	punct	),NP
R8213	T12746	T12703	cc	or,in
R8214	T12747	T12748	advmod	directly,in
R8215	T12748	T12703	conj	in,in
R8216	T12749	T12750	compound	Laemmli,bβuffer
R8217	T12750	T12748	pobj	bβuffer,in
R8218	T12751	T12694	cc	and,generated
R8219	T12752	T12694	conj	boiled,generated
R822	T1624	T1625	compound	follicle,morphogenesis
R8220	T12753	T12694	punct	.,generated
R8221	T12755	T12756	prep	For,pulverized
R8222	T12757	T12758	compound	skin,tissue
R8223	T12758	T12755	pobj	tissue,For
R8224	T12759	T12756	punct	: ,pulverized
R8225	T12760	T12761	amod	Frozen,tissue
R8226	T12761	T12756	nsubjpass	tissue,pulverized
R8227	T12762	T12756	auxpass	was,pulverized
R8228	T12763	T12756	prep	in,pulverized
R8229	T12764	T12765	det	a,Gevebesmascher
R823	T1702	T1698	amod	necessary,dynamics
R8230	T12765	T12763	pobj	Gevebesmascher,in
R8231	T12766	T12767	amod	liquid,nitrogen
R8232	T12767	T12768	npadvmod	nitrogen,cooled
R8233	T12768	T12765	amod	cooled,Gevebesmascher
R8234	T12769	T12768	punct	-,cooled
R8235	T12770	T12756	cc	and,pulverized
R8236	T12771	T12772	det	the,powder
R8237	T12772	T12773	nsubj	powder,scraped
R8238	T12773	T12756	conj	scraped,pulverized
R8239	T12774	T12773	prep	into,scraped
R824	T1625	T1611	nsubjpass	morphogenesis,blocked
R8240	T12775	T12776	det	a,tube
R8241	T12776	T12774	pobj	tube,into
R8242	T12777	T12776	amod	chilled,tube
R8243	T12778	T12776	compound	microcentrifuge,tube
R8244	T12779	T12756	punct	.,pulverized
R8245	T12781	T12782	compound	RIPA,buffer
R8246	T12782	T12783	nsubjpass	buffer,added
R8247	T12784	T12785	punct	(,X
R8248	T12785	T12782	parataxis	X,buffer
R8249	T12786	T12787	nummod	1,%
R825	T1626	T1611	auxpass	is,blocked
R8250	T12787	T12785	compound	%,X
R8251	T12788	T12785	compound	Triton,X
R8252	T12789	T12785	punct	-,X
R8253	T12790	T12785	nummod	100,X
R8254	T12791	T12785	prep	in,X
R8255	T12792	T12791	pobj	PBS,in
R8256	T12793	T12785	prep	with,X
R8257	T12794	T12795	nummod	10,mM
R8258	T12795	T12796	compound	mM,EDTA
R8259	T12796	T12793	pobj	EDTA,with
R826	T1627	T1611	punct	", ",blocked
R8260	T12797	T12796	punct	", ",EDTA
R8261	T12798	T12799	nummod	150,mN
R8262	T12799	T12800	compound	mN,NaCl
R8263	T12800	T12796	conj	NaCl,EDTA
R8264	T12801	T12800	punct	", ",NaCl
R8265	T12802	T12803	nummod	1,%
R8266	T12803	T12804	compound	%,deoxycholate
R8267	T12804	T12800	conj	deoxycholate,NaCl
R8268	T12805	T12804	compound	sodium,deoxycholate
R8269	T12806	T12804	punct	", ",deoxycholate
R827	T1703	T1704	aux	to,stabilize
R8270	T12807	T12804	cc	and,deoxycholate
R8271	T12808	T12809	nummod	0.1,%
R8272	T12809	T12810	compound	%,SDS
R8273	T12810	T12804	conj	SDS,deoxycholate
R8274	T12811	T12785	punct	),X
R8275	T12812	T12782	cc	and,buffer
R8276	T12813	T12814	compound	protease,inhibitors
R8277	T12814	T12782	conj	inhibitors,buffer
R8278	T12815	T12814	cc	or,inhibitors
R8279	T12816	T12817	compound	Laemmli,buffer
R828	T1628	T1611	advcl	suggesting,blocked
R8280	T12817	T12814	conj	buffer,inhibitors
R8281	T12818	T12783	auxpass	was,added
R8282	T12819	T12783	punct	.,added
R8283	T12821	T12822	det	The,suspension
R8284	T12822	T12824	nsubjpass	suspension,sonicated
R8285	T12823	T12822	compound	cell,suspension
R8286	T12825	T12824	auxpass	was,sonicated
R8287	T12826	T12827	nummod	three,times
R8288	T12827	T12824	npadvmod	times,sonicated
R8289	T12828	T12824	prep	for,sonicated
R829	T1629	T1630	mark	that,is
R8290	T12829	T12830	nummod	15,s
R8291	T12830	T12828	pobj	s,for
R8292	T12831	T12824	cc	and,sonicated
R8293	T12832	T12824	conj	centrifuged,sonicated
R8294	T12833	T12832	prep	at,centrifuged
R8295	T12834	T12835	nummod	"14,000",rpm
R8296	T12835	T12833	pobj	rpm,at
R8297	T12836	T12832	prep	at,centrifuged
R8298	T12837	T12838	nummod	4,°C
R8299	T12838	T12836	pobj	°C,at
R83	T237	T231	acomp	necessary,is
R830	T1630	T1628	ccomp	is,suggesting
R8300	T12839	T12824	punct	.,sonicated
R8301	T12841	T12842	det	The,supernatant
R8302	T12842	T12843	nsubjpass	supernatant,separated
R8303	T12844	T12843	auxpass	was,separated
R8304	T12845	T12843	prep	from,separated
R8305	T12846	T12847	det	the,pellet
R8306	T12847	T12845	pobj	pellet,from
R8307	T12848	T12843	cc	and,separated
R8308	T12849	T12843	conj	used,separated
R8309	T12850	T12849	prep	in,used
R831	T1704	T1702	xcomp	stabilize,necessary
R8310	T12851	T12852	det	the,experiments
R8311	T12852	T12850	pobj	experiments,in
R8312	T12853	T12843	punct	.,separated
R8313	T12855	T12856	nsubjpass	Extracts,precleared
R8314	T12857	T12855	acl	subjected,Extracts
R8315	T12858	T12857	prep	to,subjected
R8316	T12859	T12858	pobj	immunoprecipitation,to
R8317	T12860	T12856	auxpass	were,precleared
R8318	T12861	T12856	prep	with,precleared
R8319	T12862	T12863	compound	Protein,G
R832	T1631	T1632	nmod	E,cadherin
R8320	T12863	T12864	compound	G,Sepharose
R8321	T12864	T12861	pobj	Sepharose,with
R8322	T12865	T12866	punct	(,Amersham
R8323	T12866	T12864	parataxis	Amersham,Sepharose
R8324	T12867	T12866	punct	", ",Amersham
R8325	T12868	T12866	npadvmod	Piscataway,Amersham
R8326	T12869	T12866	punct	", ",Amersham
R8327	T12870	T12871	compound	New,York
R8328	T12871	T12866	npadvmod	York,Amersham
R8329	T12872	T12866	punct	", ",Amersham
R833	T1632	T1634	nmod	cadherin,regulation
R8330	T12873	T12874	compound	United,States
R8331	T12874	T12866	npadvmod	States,Amersham
R8332	T12875	T12866	punct	),Amersham
R8333	T12876	T12856	cc	and,precleared
R8334	T12877	T12856	conj	incubated,precleared
R8335	T12878	T12877	prep	with,incubated
R8336	T12879	T12878	pobj	antibody,with
R8337	T12880	T12877	prep	with,incubated
R8338	T12881	T12880	pobj	rocking,with
R8339	T12882	T12877	advmod	overnight,incubated
R834	T1705	T1706	amod	nascent,AJs
R8340	T12883	T12877	prep	at,incubated
R8341	T12884	T12885	nummod	4,°C
R8342	T12885	T12883	pobj	°C,at
R8343	T12886	T12856	punct	.,precleared
R8344	T12888	T12889	compound	Protein,G
R8345	T12889	T12890	compound	G,Sepharose
R8346	T12890	T12891	nsubjpass	Sepharose,added
R8347	T12892	T12891	auxpass	was,added
R8348	T12893	T12891	cc	and,added
R8349	T12894	T12895	nsubjpass	samples,incubated
R835	T1633	T1632	punct	-,cadherin
R8350	T12895	T12891	conj	incubated,added
R8351	T12896	T12895	auxpass	were,incubated
R8352	T12897	T12895	prep	for,incubated
R8353	T12898	T12899	nummod	1,h
R8354	T12899	T12897	pobj	h,for
R8355	T12900	T12895	prep	at,incubated
R8356	T12901	T12902	nummod	4,°C
R8357	T12902	T12900	pobj	°C,at
R8358	T12903	T12895	prep	with,incubated
R8359	T12904	T12903	pobj	rocking,with
R836	T1634	T1630	nsubj	regulation,is
R8360	T12905	T12895	punct	.,incubated
R8361	T12907	T12908	nsubjpass	Samples,washed
R8362	T12909	T12908	auxpass	were,washed
R8363	T12910	T12911	nummod	three,times
R8364	T12911	T12908	npadvmod	times,washed
R8365	T12912	T12908	prep	for,washed
R8366	T12913	T12914	nummod	5,min
R8367	T12914	T12912	pobj	min,for
R8368	T12915	T12914	advmod	each,min
R8369	T12916	T12908	prep	in,washed
R837	T1635	T1634	amod	down,regulation
R8370	T12917	T12918	compound	lysis,buffer
R8371	T12918	T12916	pobj	buffer,in
R8372	T12919	T12908	punct	", ",washed
R8373	T12920	T12908	cc	and,washed
R8374	T12921	T12922	det	the,pellet
R8375	T12922	T12930	nsubjpass	pellet,resuspended
R8376	T12923	T12924	compound	Protein,G
R8377	T12924	T12925	compound	G,antigen
R8378	T12925	T12922	compound	antigen,pellet
R8379	T12926	T12925	compound	Sepharose,antigen
R838	T1706	T1704	dobj	AJs,stabilize
R8380	T12927	T12925	punct	-,antigen
R8381	T12928	T12925	compound	antibody,antigen
R8382	T12929	T12925	punct	-,antigen
R8383	T12930	T12908	conj	resuspended,washed
R8384	T12931	T12930	auxpass	was,resuspended
R8385	T12932	T12930	prep	in,resuspended
R8386	T12933	T12934	compound	Laemmli,buffer
R8387	T12934	T12932	pobj	buffer,in
R8388	T12935	T12930	cc	and,resuspended
R8389	T12936	T12930	conj	boiled,resuspended
R839	T1636	T1634	punct	-,regulation
R8390	T12937	T12936	prep	for,boiled
R8391	T12938	T12939	nummod	10,min
R8392	T12939	T12937	pobj	min,for
R8393	T12940	T12930	punct	.,resuspended
R8394	T12942	T12943	nsubjpass	Samples,run
R8395	T12944	T12943	auxpass	were,run
R8396	T12945	T12943	prep	on,run
R8397	T12946	T12947	compound	SDS,PAGE
R8398	T12947	T12945	pobj	PAGE,on
R8399	T12948	T12947	punct	-,PAGE
R84	T238	T239	aux	to,induce
R840	T1637	T1638	det	a,event
R8400	T12949	T12943	cc	and,run
R8401	T12950	T12943	conj	transferred,run
R8402	T12951	T12950	prep	to,transferred
R8403	T12952	T12953	compound	nitrocellulose,membrane
R8404	T12953	T12951	pobj	membrane,to
R8405	T12954	T12955	punct	(,Bioscience
R8406	T12955	T12953	parataxis	Bioscience,membrane
R8407	T12956	T12955	nmod	Schleicher,Bioscience
R8408	T12957	T12956	cc	and,Schleicher
R8409	T12958	T12956	conj	Schuell,Schleicher
R841	T1638	T1630	attr	event,is
R8410	T12959	T12955	punct	", ",Bioscience
R8411	T12960	T12955	npadvmod	Keene,Bioscience
R8412	T12961	T12955	punct	", ",Bioscience
R8413	T12962	T12963	compound	New,Hampshire
R8414	T12963	T12955	npadvmod	Hampshire,Bioscience
R8415	T12964	T12955	punct	", ",Bioscience
R8416	T12965	T12966	compound	United,States
R8417	T12966	T12955	npadvmod	States,Bioscience
R8418	T12967	T12955	punct	),Bioscience
R8419	T12968	T12943	punct	.,run
R842	T1639	T1638	amod	critical,event
R8420	T12970	T12971	compound	Western,blot
R8421	T12971	T12972	compound	blot,signals
R8422	T12972	T12973	nsubjpass	signals,developed
R8423	T12974	T12973	auxpass	were,developed
R8424	T12975	T12973	advcl	using,developed
R8425	T12976	T12977	det	the,kit
R8426	T12977	T12975	dobj	kit,using
R8427	T12978	T12977	amod	enhanced,kit
R8428	T12979	T12977	compound	chemiluminescence,kit
R8429	T12980	T12977	prep	from,kit
R843	T1640	T1638	prep	in,event
R8430	T12981	T12980	pobj	Amersham,from
R8431	T13041	T13042	compound	Cell,culture
R8432	T13044	T13045	amod	Primary,keratinocytes
R8433	T13045	T13046	nsubjpass	keratinocytes,culture
R8434	T13047	T13046	auxpass	were,culture
R8435	T13048	T13046	prep	in,culture
R8436	T13049	T13050	amod	low,calcium
R8437	T13050	T13052	compound	calcium,medium
R8438	T13051	T13050	punct	-,calcium
R8439	T13052	T13048	pobj	medium,in
R844	T1707	T1704	cc	and,stabilize
R8440	T13053	T13054	mark	as,described
R8441	T13054	T13046	advcl	described,culture
R8442	T13055	T13054	advmod	previously,described
R8443	T13056	T13057	punct	[,4
R8444	T13057	T13046	parataxis	4,culture
R8445	T13058	T13057	punct	],4
R8446	T13059	T13046	punct	.,culture
R8447	T13061	T13062	amod	Transient,transfections
R8448	T13062	T13063	nsubjpass	transfections,carried
R8449	T13064	T13063	auxpass	were,carried
R845	T1708	T1704	conj	integrate,stabilize
R8450	T13065	T13063	prt	out,carried
R8451	T13066	T13063	prep	with,carried
R8452	T13067	T13068	compound	FuGENE6,reagent
R8453	T13068	T13066	pobj	reagent,with
R8454	T13069	T13070	punct	(,Roche
R8455	T13070	T13068	parataxis	Roche,reagent
R8456	T13071	T13070	punct	", ",Roche
R8457	T13072	T13070	npadvmod	Indianapolis,Roche
R8458	T13073	T13070	punct	", ",Roche
R8459	T13074	T13070	npadvmod	Indiana,Roche
R846	T1641	T1640	pcomp	governing,in
R8460	T13075	T13070	punct	", ",Roche
R8461	T13076	T13077	compound	United,States
R8462	T13077	T13070	npadvmod	States,Roche
R8463	T13078	T13070	punct	),Roche
R8464	T13079	T13063	prep	according,carried
R8465	T13080	T13079	prep	to,according
R8466	T13081	T13082	det	the,manufacturer
R8467	T13082	T13083	poss	manufacturer,protocol
R8468	T13083	T13080	pobj	protocol,to
R8469	T13084	T13082	case	's,manufacturer
R847	T1709	T1710	det	the,cytoskeleton
R8470	T13085	T13063	punct	.,carried
R8471	T13087	T13088	nsubjpass	Measurement,carried
R8472	T13089	T13087	prep	of,Measurement
R8473	T13090	T13091	nmod	β,galactosidase
R8474	T13091	T13093	nmod	galactosidase,levels
R8475	T13092	T13091	punct	-,galactosidase
R8476	T13093	T13089	pobj	levels,of
R8477	T13094	T13091	cc	or,galactosidase
R8478	T13095	T13091	conj	luciferase,galactosidase
R8479	T13096	T13088	prep	in,carried
R848	T1642	T1643	det	the,dynamics
R8480	T13097	T13098	compound	promoter,activity
R8481	T13098	T13099	compound	activity,studies
R8482	T13099	T13096	pobj	studies,in
R8483	T13100	T13088	auxpass	were,carried
R8484	T13101	T13088	prt	out,carried
R8485	T13102	T13088	prep	with,carried
R8486	T13103	T13104	det	the,kit
R8487	T13104	T13102	pobj	kit,with
R8488	T13105	T13106	compound	Galacto,Lite
R8489	T13106	T13104	compound	Lite,kit
R849	T1643	T1641	dobj	dynamics,governing
R8490	T13107	T13106	punct	-,Lite
R8491	T13108	T13104	compound	assay,kit
R8492	T13109	T13110	punct	(,TROPIX
R8493	T13110	T13104	parataxis	TROPIX,kit
R8494	T13111	T13110	punct	", ",TROPIX
R8495	T13112	T13110	npadvmod	Bedford,TROPIX
R8496	T13113	T13110	punct	", ",TROPIX
R8497	T13114	T13110	npadvmod	Massachusetts,TROPIX
R8498	T13115	T13110	punct	", ",TROPIX
R8499	T13116	T13117	compound	United,States
R85	T239	T231	advcl	induce,is
R850	T1644	T1643	compound	adhesion,dynamics
R8500	T13117	T13110	npadvmod	States,TROPIX
R8501	T13118	T13110	punct	),TROPIX
R8502	T13119	T13104	cc	and,kit
R8503	T13120	T13121	det	the,luciferase
R8504	T13121	T13104	conj	luciferase,kit
R8505	T13122	T13121	amod	Dual,luciferase
R8506	T13123	T13124	punct	(,Promega
R8507	T13124	T13121	parataxis	Promega,luciferase
R8508	T13125	T13124	punct	", ",Promega
R8509	T13126	T13124	npadvmod	Madison,Promega
R851	T1710	T1708	dobj	cytoskeleton,integrate
R8510	T13127	T13124	punct	", ",Promega
R8511	T13128	T13124	npadvmod	Wisconsin,Promega
R8512	T13129	T13124	punct	", ",Promega
R8513	T13130	T13131	compound	United,States
R8514	T13131	T13124	npadvmod	States,Promega
R8515	T13132	T13124	punct	),Promega
R8516	T13133	T13121	punct	", ",luciferase
R8517	T13134	T13088	advmod	respectively,carried
R8518	T13135	T13088	punct	.,carried
R8519	T13137	T13138	compound	Runella,luciferase
R852	T1645	T1643	amod	necessary,dynamics
R8520	T13138	T13139	nsubjpass	luciferase,cotransfected
R8521	T13140	T13139	auxpass	was,cotransfected
R8522	T13141	T13139	prep	into,cotransfected
R8523	T13142	T13141	pobj	cells,into
R8524	T13143	T13144	aux	to,correct
R8525	T13144	T13139	advcl	correct,cotransfected
R8526	T13145	T13144	prep	for,correct
R8527	T13146	T13147	compound	transfection,efficiency
R8528	T13147	T13145	pobj	efficiency,for
R8529	T13148	T13139	punct	.,cotransfected
R853	T1646	T1645	prep	for,necessary
R8530	T13150	T13151	nsubjpass	Experiments,done
R8531	T13152	T13151	auxpass	were,done
R8532	T13153	T13151	prep	in,done
R8533	T13154	T13153	pobj	triplicate,in
R8534	T13155	T13151	cc	and,done
R8535	T13156	T13151	conj	repeated,done
R8536	T13157	T13158	advmod	at,three
R8537	T13158	T13160	nummod	three,times
R8538	T13159	T13158	advmod	least,three
R8539	T13160	T13156	npadvmod	times,repeated
R854	T1647	T1648	compound	budding,morphogenesis
R8540	T13161	T13151	punct	.,done
R8541	T13163	T13164	nsubjpass	Measurements,done
R8542	T13165	T13164	auxpass	were,done
R8543	T13166	T13164	prep	on,done
R8544	T13167	T13168	det	a,luminometer
R8545	T13168	T13166	pobj	luminometer,on
R8546	T13169	T13170	punct	(,Instruments
R8547	T13170	T13168	parataxis	Instruments,luminometer
R8548	T13171	T13170	compound	MGM,Instruments
R8549	T13172	T13170	punct	", ",Instruments
R855	T1711	T1708	prep	across,integrate
R8550	T13173	T13170	npadvmod	Hamden,Instruments
R8551	T13174	T13170	punct	", ",Instruments
R8552	T13175	T13170	npadvmod	Connecticut,Instruments
R8553	T13176	T13170	punct	", ",Instruments
R8554	T13177	T13178	compound	United,States
R8555	T13178	T13170	npadvmod	States,Instruments
R8556	T13179	T13170	punct	),Instruments
R8557	T13180	T13164	punct	.,done
R8558	T13182	T13183	prep	For,starved
R8559	T13184	T13182	pobj	experiments,For
R856	T1648	T1646	pobj	morphogenesis,for
R8560	T13185	T13184	acl	measuring,experiments
R8561	T13186	T13185	dobj	phosphorylation,measuring
R8562	T13187	T13186	prep	of,phosphorylation
R8563	T13188	T13187	pobj	MAPK,of
R8564	T13189	T13183	punct	", ",starved
R8565	T13190	T13183	nsubjpass	keratinocytes,starved
R8566	T13191	T13183	auxpass	were,starved
R8567	T13192	T13183	dep	serum,starved
R8568	T13193	T13183	prep	for,starved
R8569	T13194	T13195	nummod	3,h
R857	T1649	T1650	punct	[,4
R8570	T13195	T13193	pobj	h,for
R8571	T13196	T13197	amod	prior,to
R8572	T13197	T13183	prep	to,starved
R8573	T13198	T13197	pobj	harvesting,to
R8574	T13199	T13198	prep	of,harvesting
R8575	T13200	T13199	pobj	cells,of
R8576	T13201	T13198	prep	by,harvesting
R8577	T13202	T13201	pobj	incubation,by
R8578	T13203	T13202	prep	in,incubation
R8579	T13204	T13203	pobj	medium,in
R858	T1712	T1713	det	an,sheet
R8580	T13205	T13204	acl	lacking,medium
R8581	T13206	T13205	dobj	serum,lacking
R8582	T13207	T13183	punct	.,starved
R8583	T13209	T13210	nsubjpass	Treatment,described
R8584	T13211	T13209	prep	of,Treatment
R8585	T13212	T13211	pobj	cells,of
R8586	T13213	T13209	prep	with,Treatment
R8587	T13214	T13215	npadvmod	Wnt,conditioned
R8588	T13215	T13220	amod	conditioned,medium
R8589	T13216	T13214	punct	-,Wnt
R859	T1650	T1608	parataxis	4,showed
R8590	T13217	T13214	cc	and,Wnt
R8591	T13218	T13214	conj	noggin,Wnt
R8592	T13219	T13215	punct	-,conditioned
R8593	T13220	T13213	pobj	medium,with
R8594	T13221	T13210	auxpass	was,described
R8595	T13222	T13210	advmod	previously,described
R8596	T13223	T13224	punct	[,4
R8597	T13224	T13210	parataxis	4,described
R8598	T13225	T13224	punct	],4
R8599	T13226	T13210	punct	.,described
R86	T240	T239	advmod	transiently,induce
R860	T1651	T1650	punct	],4
R8600	T13310	T13311	det	The,promoter
R8601	T13311	T13317	nsubjpass	promoter,generated
R8602	T13312	T13313	nummod	2.2,kb
R8603	T13313	T13311	nmod	kb,promoter
R8604	T13314	T13313	punct	-,kb
R8605	T13315	T13311	amod	murine,promoter
R8606	T13316	T13311	compound	Snail,promoter
R8607	T13318	T13317	auxpass	was,generated
R8608	T13319	T13317	prep	by,generated
R8609	T13320	T13319	pobj	PCR,by
R861	T1652	T1608	punct	.,showed
R8610	T13321	T13317	advcl	using,generated
R8611	T13322	T13323	det	a,primer
R8612	T13323	T13321	dobj	primer,using
R8613	T13324	T13323	amod	forward,primer
R8614	T13325	T13321	prep	with,using
R8615	T13326	T13327	det	an,sequence
R8616	T13327	T13325	pobj	sequence,with
R8617	T13328	T13327	compound	XbaI,sequence
R8618	T13329	T13327	compound	linker,sequence
R8619	T13330	T13327	punct	", ",sequence
R862	T1713	T1711	pobj	sheet,across
R8620	T13331	T13332	nummod	5,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8621	T13332	T13327	appos	TCTAGAATTGTTTGCTGCTGTATGGTCTTC,sequence
R8622	T13333	T13331	punct	′,5
R8623	T13334	T13332	punct	-,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8624	T13335	T13332	punct	-,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8625	T13336	T13332	nummod	3,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8626	T13337	T13332	punct	′,TCTAGAATTGTTTGCTGCTGTATGGTCTTC
R8627	T13338	T13327	punct	", ",sequence
R8628	T13339	T13327	cc	along,sequence
R8629	T13340	T13339	dep	with,along
R863	T1654	T1655	prep	Like,binds
R8630	T13341	T13342	det	a,primer
R8631	T13342	T13327	conj	primer,sequence
R8632	T13343	T13342	amod	reverse,primer
R8633	T13344	T13342	prep	with,primer
R8634	T13345	T13346	det	a,sequence
R8635	T13346	T13344	pobj	sequence,with
R8636	T13347	T13346	compound	BglII,sequence
R8637	T13348	T13346	compound	linker,sequence
R8638	T13349	T13346	punct	", ",sequence
R8639	T13350	T13351	nummod	5,GTAG
R864	T1714	T1713	amod	epithelial,sheet
R8640	T13351	T13346	appos	GTAG,sequence
R8641	T13352	T13350	punct	′,5
R8642	T13353	T13351	punct	-,GTAG
R8643	T13354	T13351	compound	AGATCTGTTGGCCAGAGCGACCTAG,GTAG
R8644	T13355	T13351	punct	-,GTAG
R8645	T13356	T13351	punct	-,GTAG
R8646	T13357	T13351	nummod	3,GTAG
R8647	T13358	T13351	punct	′,GTAG
R8648	T13359	T13342	punct	", ",primer
R8649	T13360	T13342	cc	and,primer
R865	T1656	T1654	pobj	LEF,Like
R8650	T13361	T13362	nmod	mouse,DNA
R8651	T13362	T13342	conj	DNA,primer
R8652	T13363	T13362	amod	genomic,DNA
R8653	T13364	T13362	prep	as,DNA
R8654	T13365	T13366	det	a,template
R8655	T13366	T13364	pobj	template,as
R8656	T13367	T13317	punct	.,generated
R8657	T13369	T13370	det	The,product
R8658	T13370	T13372	nsubjpass	product,purified
R8659	T13371	T13370	compound	PCR,product
R866	T1657	T1656	punct	-,LEF
R8660	T13373	T13372	auxpass	was,purified
R8661	T13374	T13372	prep	with,purified
R8662	T13375	T13376	det	the,Kit
R8663	T13376	T13374	pobj	Kit,with
R8664	T13377	T13378	compound	Gel,Extraction
R8665	T13378	T13376	compound	Extraction,Kit
R8666	T13379	T13380	punct	(,Qiagen
R8667	T13380	T13376	parataxis	Qiagen,Kit
R8668	T13381	T13380	punct	", ",Qiagen
R8669	T13382	T13380	npadvmod	Valencia,Qiagen
R867	T1715	T1716	punct	[,8
R8670	T13383	T13380	punct	", ",Qiagen
R8671	T13384	T13380	npadvmod	California,Qiagen
R8672	T13385	T13380	punct	", ",Qiagen
R8673	T13386	T13387	compound	United,States
R8674	T13387	T13380	npadvmod	States,Qiagen
R8675	T13388	T13380	punct	),Qiagen
R8676	T13389	T13372	cc	and,purified
R8677	T13390	T13372	conj	ligated,purified
R8678	T13391	T13390	prep	into,ligated
R8679	T13392	T13393	compound	pCRII,TOPO
R868	T1658	T1656	nummod	1,LEF
R8680	T13393	T13395	compound	TOPO,vector
R8681	T13394	T13393	punct	-,TOPO
R8682	T13395	T13391	pobj	vector,into
R8683	T13396	T13395	compound	TA,vector
R8684	T13397	T13398	punct	(,Invitrogen
R8685	T13398	T13395	parataxis	Invitrogen,vector
R8686	T13399	T13398	punct	", ",Invitrogen
R8687	T13400	T13398	npadvmod	Carlsbad,Invitrogen
R8688	T13401	T13398	punct	", ",Invitrogen
R8689	T13402	T13398	npadvmod	California,Invitrogen
R869	T1659	T1655	punct	", ",binds
R8690	T13403	T13398	punct	", ",Invitrogen
R8691	T13404	T13405	compound	United,States
R8692	T13405	T13398	npadvmod	States,Invitrogen
R8693	T13406	T13398	punct	),Invitrogen
R8694	T13407	T13372	punct	.,purified
R8695	T13409	T13410	det	The,promoter
R8696	T13410	T13411	nsubjpass	promoter,verified
R8697	T13412	T13411	auxpass	was,verified
R8698	T13413	T13411	prep	by,verified
R8699	T13414	T13413	pobj	sequencing,by
R87	T241	T242	det	the,Snail
R870	T1660	T1661	compound	E,cadherin
R8700	T13415	T13411	cc	and,verified
R8701	T13416	T13411	conj	digested,verified
R8702	T13417	T13416	prep	with,digested
R8703	T13418	T13417	pobj	XbaI,with
R8704	T13419	T13418	cc	and,XbaI
R8705	T13420	T13418	conj	BglII,XbaI
R8706	T13421	T13416	cc	and,digested
R8707	T13422	T13416	conj	subcloned,digested
R8708	T13423	T13422	prep	into,subcloned
R8709	T13424	T13425	det	the,vector
R871	T1661	T1655	nsubj	cadherin,binds
R8710	T13425	T13423	pobj	vector,into
R8711	T13426	T13427	compound	pβ,gal
R8712	T13427	T13425	compound	gal,vector
R8713	T13428	T13427	punct	-,gal
R8714	T13429	T13425	compound	BASIC,vector
R8715	T13430	T13431	punct	(,Clontech
R8716	T13431	T13425	parataxis	Clontech,vector
R8717	T13432	T13431	compound	BD,Clontech
R8718	T13433	T13431	compound	Biosciences,Clontech
R8719	T13434	T13431	punct	", ",Clontech
R872	T1716	T1694	parataxis	8,coordinates
R8720	T13435	T13436	compound	Palo,Alto
R8721	T13436	T13431	npadvmod	Alto,Clontech
R8722	T13437	T13431	punct	", ",Clontech
R8723	T13438	T13431	npadvmod	California,Clontech
R8724	T13439	T13431	punct	", ",Clontech
R8725	T13440	T13441	compound	United,States
R8726	T13441	T13431	npadvmod	States,Clontech
R8727	T13442	T13431	punct	),Clontech
R8728	T13443	T13411	punct	.,verified
R8729	T13445	T13446	det	The,mutations
R873	T1662	T1661	punct	-,cadherin
R8730	T13446	T13448	nsubjpass	mutations,generated
R8731	T13447	T13446	compound	point,mutations
R8732	T13449	T13446	prep	in,mutations
R8733	T13450	T13451	det	the,element
R8734	T13451	T13449	pobj	element,in
R8735	T13452	T13451	compound	SMAD,element
R8736	T13453	T13451	compound	binding,element
R8737	T13454	T13448	auxpass	was,generated
R8738	T13455	T13448	prep	with,generated
R8739	T13456	T13457	det	the,Kit
R874	T1663	T1655	advmod	also,binds
R8740	T13457	T13455	pobj	Kit,with
R8741	T13458	T13459	compound	Quik,Change
R8742	T13459	T13457	compound	Change,Kit
R8743	T13460	T13459	punct	-,Change
R8744	T13461	T13462	punct	(,Stratagene
R8745	T13462	T13457	parataxis	Stratagene,Kit
R8746	T13463	T13462	punct	", ",Stratagene
R8747	T13464	T13465	compound	La,Jolla
R8748	T13465	T13462	npadvmod	Jolla,Stratagene
R8749	T13466	T13462	punct	", ",Stratagene
R875	T1717	T1716	nummod	6,8
R8750	T13467	T13462	npadvmod	California,Stratagene
R8751	T13468	T13462	punct	", ",Stratagene
R8752	T13469	T13470	compound	United,States
R8753	T13470	T13462	npadvmod	States,Stratagene
R8754	T13471	T13462	punct	),Stratagene
R8755	T13472	T13448	advcl	using,generated
R8756	T13473	T13474	det	the,primer
R8757	T13474	T13472	dobj	primer,using
R8758	T13475	T13474	amod	forward,primer
R8759	T13476	T13477	nummod	5,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R876	T1664	T1655	prep	to,binds
R8760	T13477	T13474	appos	GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC,primer
R8761	T13478	T13476	punct	′,5
R8762	T13479	T13477	punct	-,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8763	T13480	T13477	punct	-,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8764	T13481	T13477	nummod	3,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8765	T13482	T13477	punct	′,GGGCGGGCTTAGGTGTTTTCATTTACTCTTGAGGAAAAGCTTGGC
R8766	T13483	T13474	cc	and,primer
R8767	T13484	T13485	det	the,primer
R8768	T13485	T13474	conj	primer,primer
R8769	T13486	T13485	amod	reverse,primer
R877	T1665	T1666	compound	β,catenin
R8770	T13487	T13488	nummod	5,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8771	T13488	T13485	appos	CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC,primer
R8772	T13489	T13487	punct	′,5
R8773	T13490	T13488	punct	-,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8774	T13491	T13488	compound	GCTTTT,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8775	T13492	T13488	punct	-,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8776	T13493	T13488	punct	-,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8777	T13494	T13488	nummod	3,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8778	T13495	T13488	punct	′,CCTCAAGAGTAAATGAAAACACCTAAGCCCGCCCTGCCC
R8779	T13496	T13448	punct	.,generated
R878	T1666	T1664	pobj	catenin,to
R8780	T13498	T13499	det	The,probes
R8781	T13499	T13500	nsubjpass	probes,generated
R8782	T13501	T13499	prep	for,probes
R8783	T13502	T13503	det	the,hybridization
R8784	T13503	T13501	pobj	hybridization,for
R8785	T13504	T13503	nmod	Snail,hybridization
R8786	T13505	T13506	advmod	in,situ
R8787	T13506	T13503	amod	situ,hybridization
R8788	T13507	T13500	auxpass	were,generated
R8789	T13508	T13500	prep	against,generated
R879	T1718	T1716	punct	",",8
R8790	T13509	T13510	det	the,UTR
R8791	T13510	T13508	pobj	UTR,against
R8792	T13511	T13510	nummod	3,UTR
R8793	T13512	T13511	punct	′,3
R8794	T13513	T13500	prep	by,generated
R8795	T13514	T13513	pobj	PCR,by
R8796	T13515	T13500	advcl	using,generated
R8797	T13516	T13517	det	the,primer
R8798	T13517	T13515	dobj	primer,using
R8799	T13518	T13517	amod	forward,primer
R88	T242	T239	dobj	Snail,induce
R880	T1667	T1666	punct	-,catenin
R8800	T13519	T13520	nummod	5,ACCTTCTCCCGCATGTCCTTGCTCC
R8801	T13520	T13517	appos	ACCTTCTCCCGCATGTCCTTGCTCC,primer
R8802	T13521	T13519	punct	′,5
R8803	T13522	T13520	punct	-,ACCTTCTCCCGCATGTCCTTGCTCC
R8804	T13523	T13520	punct	-,ACCTTCTCCCGCATGTCCTTGCTCC
R8805	T13524	T13520	nummod	3,ACCTTCTCCCGCATGTCCTTGCTCC
R8806	T13525	T13520	punct	′,ACCTTCTCCCGCATGTCCTTGCTCC
R8807	T13526	T13517	cc	and,primer
R8808	T13527	T13528	det	the,primer
R8809	T13528	T13517	conj	primer,primer
R881	T1668	T1655	punct	.,binds
R8810	T13529	T13528	amod	reverse,primer
R8811	T13530	T13531	nummod	5,CTGCTGAGGCATGGTTACAGCTGG
R8812	T13531	T13528	appos	CTGCTGAGGCATGGTTACAGCTGG,primer
R8813	T13532	T13530	punct	′,5
R8814	T13533	T13531	punct	-,CTGCTGAGGCATGGTTACAGCTGG
R8815	T13534	T13531	punct	-,CTGCTGAGGCATGGTTACAGCTGG
R8816	T13535	T13531	nummod	3,CTGCTGAGGCATGGTTACAGCTGG
R8817	T13536	T13531	punct	′,CTGCTGAGGCATGGTTACAGCTGG
R8818	T13537	T13528	punct	", ",primer
R8819	T13538	T13528	cc	and,primer
R882	T1719	T1716	nummod	7,8
R8820	T13539	T13540	amod	genomic,DNA
R8821	T13540	T13528	conj	DNA,primer
R8822	T13541	T13540	prep	as,DNA
R8823	T13542	T13543	det	a,template
R8824	T13543	T13541	pobj	template,as
R8825	T13544	T13500	punct	.,generated
R8826	T13546	T13547	det	The,product
R8827	T13547	T13549	nsubjpass	product,purified
R8828	T13548	T13547	compound	PCR,product
R8829	T13550	T13549	auxpass	was,purified
R883	T1670	T1671	prep	At,recruit
R8830	T13551	T13549	dep	gel,purified
R8831	T13552	T13549	cc	and,purified
R8832	T13553	T13549	conj	ligated,purified
R8833	T13554	T13553	prep	into,ligated
R8834	T13555	T13556	compound	pCRII,TOPO
R8835	T13556	T13558	compound	TOPO,vector
R8836	T13557	T13556	punct	-,TOPO
R8837	T13558	T13554	pobj	vector,into
R8838	T13559	T13558	compound	TA,vector
R8839	T13560	T13549	punct	.,purified
R884	T1720	T1716	punct	",",8
R8840	T13562	T13563	det	The,domain
R8841	T13563	T13565	nsubjpass	domain,generated
R8842	T13564	T13563	amod	pre-LIM,domain
R8843	T13566	T13563	prep	of,domain
R8844	T13567	T13566	pobj	Ajuba,of
R8845	T13568	T13565	auxpass	was,generated
R8846	T13569	T13565	advmod	essentially,generated
R8847	T13570	T13571	mark	as,described
R8848	T13571	T13565	advcl	described,generated
R8849	T13572	T13573	punct	[,9
R885	T1672	T1670	pobj	sites,At
R8850	T13573	T13571	parataxis	9,described
R8851	T13574	T13573	punct	],9
R8852	T13575	T13565	punct	", ",generated
R8853	T13576	T13565	cc	but,generated
R8854	T13577	T13578	auxpass	was,fused
R8855	T13578	T13565	conj	fused,generated
R8856	T13579	T13578	prep	to,fused
R8857	T13580	T13579	pobj	GFP,to
R8858	T13581	T13578	prep	by,fused
R8859	T13582	T13581	pcomp	subcloning,by
R886	T1673	T1672	prep	of,sites
R8860	T13583	T13582	prep	from,subcloning
R8861	T13584	T13585	det	the,vector
R8862	T13585	T13583	pobj	vector,from
R8863	T13586	T13587	nmod	pEGFP,N1
R8864	T13587	T13585	nmod	N1,vector
R8865	T13588	T13587	punct	-,N1
R8866	T13589	T13585	nummod	20,vector
R8867	T13590	T13591	punct	(,Clontech
R8868	T13591	T13585	parataxis	Clontech,vector
R8869	T13592	T13591	compound	BD,Clontech
R887	T1674	T1675	compound	cell,cell
R8870	T13593	T13591	compound	Biosciences,Clontech
R8871	T13594	T13591	punct	),Clontech
R8874	T13649	T13650	advmod	In,situ
R8875	T13650	T13651	amod	situ,hybridization
R8876	T13653	T13654	det	The,vector
R8877	T13654	T13659	nsubjpass	vector,used
R8878	T13655	T13656	compound	pCRII,TOPO
R8879	T13656	T13654	compound	TOPO,vector
R888	T1721	T1716	punct	],8
R8880	T13657	T13656	punct	-,TOPO
R8881	T13658	T13654	compound	TA,vector
R8882	T13660	T13654	acl	containing,vector
R8883	T13661	T13662	det	a,region
R8884	T13662	T13660	dobj	region,containing
R8885	T13663	T13662	prep	of,region
R8886	T13664	T13665	det	the,UTR
R8887	T13665	T13663	pobj	UTR,of
R8888	T13666	T13665	nummod	3,UTR
R8889	T13667	T13666	punct	′,3
R889	T1722	T1671	punct	.,recruit
R8890	T13668	T13665	prep	of,UTR
R8891	T13669	T13668	pobj	Snail,of
R8892	T13670	T13659	auxpass	was,used
R8893	T13671	T13659	prep	as,used
R8894	T13672	T13673	det	a,template
R8895	T13673	T13671	pobj	template,as
R8896	T13674	T13675	aux	to,generate
R8897	T13675	T13659	advcl	generate,used
R8898	T13676	T13677	npadvmod	digoxigenin,labeled
R8899	T13677	T13679	amod	labeled,riboprobes
R89	T243	T242	compound	transcription,Snail
R890	T1724	T1725	compound	α,Catenin
R8900	T13678	T13677	punct	-,labeled
R8901	T13679	T13675	dobj	riboprobes,generate
R8902	T13680	T13679	nmod	sense,riboprobes
R8903	T13681	T13680	cc	and,sense
R8904	T13682	T13680	conj	antisense,sense
R8905	T13683	T13684	punct	(,Roche
R8906	T13684	T13679	parataxis	Roche,riboprobes
R8907	T13685	T13684	punct	),Roche
R8908	T13686	T13659	punct	.,used
R8909	T13688	T13689	det	The,probes
R891	T1725	T1727	nsubj	Catenin,binds
R8910	T13689	T13691	nsubjpass	probes,obtained
R8911	T13690	T13689	amod	respective,probes
R8912	T13692	T13691	auxpass	were,obtained
R8913	T13693	T13691	prep	by,obtained
R8914	T13694	T13695	nmod	XhoI,digestions
R8915	T13695	T13693	pobj	digestions,by
R8916	T13696	T13694	cc	and,XhoI
R8917	T13697	T13694	conj	BamH1,XhoI
R8918	T13698	T13691	punct	.,obtained
R8919	T13700	T13701	advmod	In,situ
R892	T1726	T1725	punct	-,Catenin
R8920	T13701	T13702	amod	situ,hybridizations
R8921	T13702	T13703	nsubjpass	hybridizations,performed
R8922	T13704	T13703	auxpass	were,performed
R8923	T13705	T13703	prep	on,performed
R8924	T13706	T13707	nummod	10,μm
R8925	T13707	T13709	npadvmod	μm,thick
R8926	T13708	T13707	punct	-,μm
R8927	T13709	T13710	amod	thick,sections
R8928	T13710	T13705	pobj	sections,on
R8929	T13711	T13710	prep	of,sections
R893	T1780	T1779	compound	protein,kinase
R8930	T13712	T13713	compound	E17.5,embryos
R8931	T13713	T13711	pobj	embryos,of
R8932	T13714	T13713	compound	mouse,embryos
R8933	T13715	T13703	punct	.,performed
R8934	T13717	T13718	det	The,sections
R8935	T13718	T13719	nsubjpass	sections,fixed
R8936	T13720	T13719	auxpass	were,fixed
R8937	T13721	T13719	prep	with,fixed
R8938	T13722	T13723	nummod	4,%
R8939	T13723	T13724	compound	%,PFA
R894	T1781	T1779	punct	(,kinase
R8940	T13724	T13721	pobj	PFA,with
R8941	T13725	T13719	prep	for,fixed
R8942	T13726	T13727	nummod	10,min
R8943	T13727	T13725	pobj	min,for
R8944	T13728	T13719	prep	at,fixed
R8945	T13729	T13730	compound	room,temperature
R8946	T13730	T13728	pobj	temperature,at
R8947	T13731	T13719	punct	", ",fixed
R8948	T13732	T13719	conj	prehybridized,fixed
R8949	T13733	T13732	prep	at,prehybridized
R895	T1782	T1779	appos	MAPK,kinase
R8950	T13734	T13735	compound	room,temperature
R8951	T13735	T13733	pobj	temperature,at
R8952	T13736	T13732	prep	for,prehybridized
R8953	T13737	T13738	nummod	4.5,h
R8954	T13738	T13736	pobj	h,for
R8955	T13739	T13732	punct	", ",prehybridized
R8956	T13740	T13732	conj	hybridized,prehybridized
R8957	T13741	T13740	prep	with,hybridized
R8958	T13742	T13743	det	the,probe
R8959	T13743	T13741	pobj	probe,with
R896	T1728	T1727	advmod	also,binds
R8960	T13744	T13745	punct	(,μg
R8961	T13745	T13743	parataxis	μg,probe
R8962	T13746	T13745	nummod	2,μg
R8963	T13747	T13748	punct	/,ml
R8964	T13748	T13745	prep	ml,μg
R8965	T13749	T13745	punct	),μg
R8966	T13750	T13740	prep	at,hybridized
R8967	T13751	T13752	nummod	55,°C
R8968	T13752	T13750	pobj	°C,at
R8969	T13753	T13740	prep	for,hybridized
R897	T1783	T1773	punct	),pathway
R8970	T13754	T13755	quantmod	12,14
R8971	T13755	T13757	nummod	14,h
R8972	T13756	T13755	punct	–,14
R8973	T13757	T13753	pobj	h,for
R8974	T13758	T13740	punct	", ",hybridized
R8975	T13759	T13740	conj	blocked,hybridized
R8976	T13760	T13759	prep	with,blocked
R8977	T13761	T13762	nummod	10,%
R8978	T13762	T13763	compound	%,NGS
R8979	T13763	T13760	pobj	NGS,with
R898	T1784	T1785	punct	[,10
R8980	T13764	T13759	punct	", ",blocked
R8981	T13765	T13759	cc	and,blocked
R8982	T13766	T13759	conj	treated,blocked
R8983	T13767	T13766	prep	with,treated
R8984	T13768	T13769	amod	anti-dig,antibody
R8985	T13769	T13767	pobj	antibody,with
R8986	T13770	T13771	compound	Fab,AP
R8987	T13771	T13769	compound	AP,antibody
R8988	T13772	T13771	punct	-,AP
R8989	T13773	T13774	punct	(,Roche
R899	T1729	T1727	prep	to,binds
R8990	T13774	T13769	parataxis	Roche,antibody
R8991	T13775	T13774	punct	#,Roche
R8992	T13776	T13774	nummod	1093274,Roche
R8993	T13777	T13774	punct	),Roche
R8994	T13778	T13766	prep	at,treated
R8995	T13779	T13780	det	a,dilution
R8996	T13780	T13778	pobj	dilution,at
R8997	T13781	T13782	quantmod	1,"2,500"
R8998	T13782	T13780	nummod	"2,500",dilution
R8999	T13783	T13782	punct	:,"2,500"
R9	T156	T147	prep	in,Pathway
R90	T244	T242	compound	factor,Snail
R900	T1785	T1727	parataxis	10,binds
R9000	T13784	T13766	prep	for,treated
R9001	T13785	T13786	nummod	3,h
R9002	T13786	T13784	pobj	h,for
R9003	T13787	T13719	punct	.,fixed
R9004	T13789	T13790	det	The,sections
R9005	T13790	T13791	nsubjpass	sections,incubated
R9006	T13792	T13791	auxpass	were,incubated
R9007	T13793	T13791	prep	with,incubated
R9008	T13794	T13793	pobj	NBT,with
R9009	T13795	T13794	cc	and,NBT
R901	T1786	T1785	nummod	9,10
R9010	T13796	T13794	conj	BCIP,NBT
R9011	T13797	T13798	mark	until,detected
R9012	T13798	T13791	advcl	detected,incubated
R9013	T13799	T13800	amod	adequate,signal
R9014	T13800	T13798	nsubjpass	signal,detected
R9015	T13801	T13798	auxpass	was,detected
R9016	T13802	T13791	punct	.,incubated
R9017	T13829	T13828	cc	and,Immunofluorescence
R9018	T13830	T13828	conj	immunohistochemistry,Immunofluorescence
R9019	T13832	T13833	compound	Tissue,samples
R902	T1730	T1731	det	the,protein
R9020	T13833	T13834	nsubjpass	samples,frozen
R9021	T13835	T13833	prep	for,samples
R9022	T13836	T13835	pobj	immunofluorescence,for
R9023	T13837	T13834	auxpass	were,frozen
R9024	T13838	T13834	prep	in,frozen
R9025	T13839	T13838	pobj	OCT,in
R9026	T13840	T13834	cc	and,frozen
R9027	T13841	T13834	conj	sectioned,frozen
R9028	T13842	T13843	nummod	10,μm
R9029	T13843	T13844	npadvmod	μm,thick
R903	T1787	T1785	punct	",",10
R9030	T13844	T13841	advcl	thick,sectioned
R9031	T13845	T13841	prep	on,sectioned
R9032	T13846	T13847	det	a,cryostat
R9033	T13847	T13845	pobj	cryostat,on
R9034	T13848	T13834	punct	.,frozen
R9035	T13850	T13851	nsubjpass	Sections,fixed
R9036	T13852	T13851	auxpass	were,fixed
R9037	T13853	T13851	prep	in,fixed
R9038	T13854	T13855	nummod	4,%
R9039	T13855	T13856	compound	%,paraformaldehyde
R904	T1788	T1785	punct	],10
R9040	T13856	T13853	pobj	paraformaldehyde,in
R9041	T13857	T13851	prep	for,fixed
R9042	T13858	T13859	nummod	10,min
R9043	T13859	T13857	pobj	min,for
R9044	T13860	T13851	prep	at,fixed
R9045	T13861	T13862	compound	room,temperature
R9046	T13862	T13860	pobj	temperature,at
R9047	T13863	T13851	punct	", ",fixed
R9048	T13864	T13851	conj	blocked,fixed
R9049	T13865	T13864	punct	", ",blocked
R905	T1731	T1729	pobj	protein,to
R9050	T13866	T13864	cc	and,blocked
R9051	T13867	T13864	conj	stained,blocked
R9052	T13868	T13867	prep	with,stained
R9053	T13869	T13868	pobj	antibodies,with
R9054	T13870	T13851	punct	.,fixed
R9055	T13872	T13873	compound	Tissue,samples
R9056	T13873	T13874	nsubjpass	samples,fixed
R9057	T13875	T13873	prep	for,samples
R9058	T13876	T13875	pobj	immunohistochemistry,for
R9059	T13877	T13874	auxpass	were,fixed
R906	T1789	T1727	punct	.,binds
R9060	T13878	T13874	prep	in,fixed
R9061	T13879	T13880	nummod	4,%
R9062	T13880	T13881	compound	%,paraformaldehyde
R9063	T13881	T13878	pobj	paraformaldehyde,in
R9064	T13882	T13874	punct	", ",fixed
R9065	T13883	T13874	conj	dehydrated,fixed
R9066	T13884	T13883	punct	", ",dehydrated
R9067	T13885	T13883	cc	and,dehydrated
R9068	T13886	T13883	conj	embedded,dehydrated
R9069	T13887	T13886	prep	in,embedded
R907	T1732	T1731	nmod	class,protein
R9070	T13888	T13887	pobj	paraffin,in
R9071	T13889	T13874	punct	.,fixed
R9072	T13891	T13892	nsubjpass	Samples,sectioned
R9073	T13893	T13892	auxpass	were,sectioned
R9074	T13894	T13892	prep	on,sectioned
R9075	T13895	T13896	det	a,microtome
R9076	T13896	T13894	pobj	microtome,on
R9077	T13897	T13898	punct	(,thick
R9078	T13898	T13892	parataxis	thick,sectioned
R9079	T13899	T13900	nummod	10,μm
R908	T1791	T1792	prep	Through,appear
R9080	T13900	T13898	npadvmod	μm,thick
R9081	T13901	T13898	punct	),thick
R9082	T13902	T13892	cc	and,sectioned
R9083	T13903	T13892	conj	rehydrated,sectioned
R9084	T13904	T13903	prep	prior,rehydrated
R9085	T13905	T13904	pcomp	to,prior
R9086	T13906	T13904	pcomp	staining,prior
R9087	T13907	T13906	prep	with,staining
R9088	T13908	T13907	pobj	antibody,with
R9089	T13909	T13892	punct	.,sectioned
R909	T1793	T1794	det	these,links
R9090	T13911	T13912	nsubjpass	Samples,unmasked
R9091	T13913	T13911	acl	stained,Samples
R9092	T13914	T13913	prep	with,stained
R9093	T13915	T13914	pobj	Snail,with
R9094	T13916	T13915	punct	", ",Snail
R9095	T13917	T13915	conj	pMAPK,Snail
R9096	T13918	T13917	punct	", ",pMAPK
R9097	T13919	T13917	conj	pSmad2,pMAPK
R9098	T13920	T13919	punct	", ",pSmad2
R9099	T13921	T13919	cc	and,pSmad2
R91	T245	T239	cc	and,induce
R910	T1733	T1732	nummod	III,class
R9100	T13922	T13923	compound	cyclin,D
R9101	T13923	T13919	conj	D,pSmad2
R9102	T13924	T13912	auxpass	were,unmasked
R9103	T13925	T13912	dep	antigen,unmasked
R9104	T13926	T13912	prep	with,unmasked
R9105	T13927	T13928	nummod	10,mM
R9106	T13928	T13929	compound	mM,citrate
R9107	T13929	T13926	pobj	citrate,with
R9108	T13930	T13929	compound	sodium,citrate
R9109	T13931	T13929	punct	(,citrate
R911	T1794	T1791	pobj	links,Through
R9110	T13932	T13929	npadvmod	pH,citrate
R9111	T13933	T13932	nummod	6,pH
R9112	T13934	T13929	punct	),citrate
R9113	T13935	T13912	prep	in,unmasked
R9114	T13936	T13937	det	an,Retriever
R9115	T13937	T13935	pobj	Retriever,in
R9116	T13938	T13937	compound	Antigen,Retriever
R9117	T13939	T13937	nummod	2100,Retriever
R9118	T13940	T13941	punct	(,Laboratories
R9119	T13941	T13937	parataxis	Laboratories,Retriever
R912	T1795	T1792	punct	", ",appear
R9120	T13942	T13941	compound	Pickcell,Laboratories
R9121	T13943	T13941	punct	", ",Laboratories
R9122	T13944	T13941	npadvmod	Leiden,Laboratories
R9123	T13945	T13941	punct	", ",Laboratories
R9124	T13946	T13941	npadvmod	Netherlands,Laboratories
R9125	T13947	T13941	punct	),Laboratories
R9126	T13948	T13912	punct	.,unmasked
R9127	T13950	T13951	det	The,kit
R9128	T13951	T13954	nsubjpass	kit,used
R9129	T13952	T13953	compound	DAB,substrate
R913	T1734	T1731	nmod	Lin,protein
R9130	T13953	T13951	compound	substrate,kit
R9131	T13955	T13956	punct	(,Labs
R9132	T13956	T13951	parataxis	Labs,kit
R9133	T13957	T13956	compound	Vector,Labs
R9134	T13958	T13956	punct	),Labs
R9135	T13959	T13954	auxpass	was,used
R9136	T13960	T13954	prep	according,used
R9137	T13961	T13960	prep	to,according
R9138	T13962	T13963	poss	manufacturer,instructions
R9139	T13963	T13961	pobj	instructions,to
R914	T1796	T1792	nsubj	AJs,appear
R9140	T13964	T13962	case	's,manufacturer
R9141	T13965	T13966	aux	to,develop
R9142	T13966	T13954	advcl	develop,used
R9143	T13967	T13968	det	the,signal
R9144	T13968	T13966	dobj	signal,develop
R9145	T13969	T13954	punct	.,used
R9146	T14008	T14009	compound	RT,PCR
R9147	T14010	T14009	punct	-,PCR
R9148	T14012	T14013	nsubjpass	RNA,extracted
R9149	T14014	T14013	auxpass	was,extracted
R915	T1797	T1792	oprd	able,appear
R9150	T14015	T14013	prep	from,extracted
R9151	T14016	T14015	pobj	keratinocytes,from
R9152	T14017	T14016	cc	or,keratinocytes
R9153	T14018	T14019	compound	skin,tissue
R9154	T14019	T14016	conj	tissue,keratinocytes
R9155	T14020	T14013	prep	with,extracted
R9156	T14021	T14020	pobj	Trizol,with
R9157	T14022	T14023	punct	(,Invitrogen
R9158	T14023	T14021	parataxis	Invitrogen,Trizol
R9159	T14024	T14023	punct	),Invitrogen
R916	T1798	T1799	aux	to,couple
R9160	T14025	T14013	prep	according,extracted
R9161	T14026	T14025	prep	to,according
R9162	T14027	T14028	det	the,manufacturer
R9163	T14028	T14029	poss	manufacturer,protocol
R9164	T14029	T14026	pobj	protocol,to
R9165	T14030	T14028	case	's,manufacturer
R9166	T14031	T14013	punct	.,extracted
R9167	T14033	T14034	nsubjpass	cDNA,generated
R9168	T14035	T14034	auxpass	was,generated
R9169	T14036	T14034	advcl	using,generated
R917	T1735	T1734	punct	-,Lin
R9170	T14037	T14038	compound	oligo,dT
R9171	T14038	T14040	compound	dT,primers
R9172	T14039	T14038	punct	-,dT
R9173	T14040	T14036	dobj	primers,using
R9174	T14041	T14040	cc	and,primers
R9175	T14042	T14043	det	the,kit
R9176	T14043	T14040	conj	kit,primers
R9177	T14044	T14043	nmod	Superscript,kit
R9178	T14045	T14044	nummod	II,Superscript
R9179	T14046	T14047	punct	(,Invitrogen
R918	T1799	T1797	xcomp	couple,able
R9180	T14047	T14043	parataxis	Invitrogen,kit
R9181	T14048	T14047	punct	),Invitrogen
R9182	T14049	T14034	punct	.,generated
R9183	T14051	T14052	det	The,primers
R9184	T14052	T14053	nsubj	primers,were
R9185	T14054	T14052	acl	used,primers
R9186	T14055	T14054	prep	for,used
R9187	T14056	T14055	pobj	PCR,for
R9188	T14057	T14058	compound	Snail,forward
R9189	T14058	T14053	attr	forward,were
R919	T1800	T1799	dobj	adhesion,couple
R9190	T14059	T14058	punct	: ,forward
R9191	T14060	T14061	nummod	5,CAGCTGGCCAGGCTCTCGGT
R9192	T14061	T14058	appos	CAGCTGGCCAGGCTCTCGGT,forward
R9193	T14062	T14060	punct	′,5
R9194	T14063	T14061	punct	-,CAGCTGGCCAGGCTCTCGGT
R9195	T14064	T14061	punct	-,CAGCTGGCCAGGCTCTCGGT
R9196	T14065	T14061	nummod	3,CAGCTGGCCAGGCTCTCGGT
R9197	T14066	T14061	punct	′,CAGCTGGCCAGGCTCTCGGT
R9198	T14067	T14058	punct	;,forward
R9199	T14068	T14069	compound	Snail,reverse
R92	T246	T239	conj	activate,induce
R920	T1736	T1734	nummod	1,Lin
R9200	T14069	T14058	conj	reverse,forward
R9201	T14070	T14069	punct	: ,reverse
R9202	T14071	T14072	nummod	5,GCGAGGGCCTCCGGAGCA
R9203	T14072	T14069	appos	GCGAGGGCCTCCGGAGCA,reverse
R9204	T14073	T14071	punct	′,5
R9205	T14074	T14072	punct	-,GCGAGGGCCTCCGGAGCA
R9206	T14075	T14072	punct	-,GCGAGGGCCTCCGGAGCA
R9207	T14076	T14072	nummod	3,GCGAGGGCCTCCGGAGCA
R9208	T14077	T14072	punct	′,GCGAGGGCCTCCGGAGCA
R9209	T14078	T14069	punct	;,reverse
R921	T1801	T1799	prep	with,couple
R9210	T14079	T14080	compound	GAPDH,forward
R9211	T14080	T14069	conj	forward,reverse
R9212	T14081	T14082	nummod	5,CGTAGACAAAATGGTGAAGGTCGG
R9213	T14082	T14080	appos	CGTAGACAAAATGGTGAAGGTCGG,forward
R9214	T14083	T14081	punct	′,5
R9215	T14084	T14082	punct	-,CGTAGACAAAATGGTGAAGGTCGG
R9216	T14085	T14082	punct	-,CGTAGACAAAATGGTGAAGGTCGG
R9217	T14086	T14082	nummod	3,CGTAGACAAAATGGTGAAGGTCGG
R9218	T14087	T14082	punct	′,CGTAGACAAAATGGTGAAGGTCGG
R9219	T14088	T14080	punct	;,forward
R922	T1802	T1803	amod	cytoskeletal,dynamics
R9220	T14089	T14080	cc	and,forward
R9221	T14090	T14091	compound	GAPDH,reverse
R9222	T14091	T14080	conj	reverse,forward
R9223	T14092	T14091	punct	: ,reverse
R9224	T14093	T14094	nummod	5,AAGCAGTTGGTGGTGCAGGATG
R9225	T14094	T14091	appos	AAGCAGTTGGTGGTGCAGGATG,reverse
R9226	T14095	T14093	punct	′,5
R9227	T14096	T14094	punct	-,AAGCAGTTGGTGGTGCAGGATG
R9228	T14097	T14094	punct	-,AAGCAGTTGGTGGTGCAGGATG
R9229	T14098	T14094	nummod	3,AAGCAGTTGGTGGTGCAGGATG
R923	T1803	T1801	pobj	dynamics,with
R9230	T14099	T14094	punct	′,AAGCAGTTGGTGGTGCAGGATG
R9231	T14100	T14053	punct	.,were
R924	T1737	T1734	punct	", ",Lin
R925	T1804	T1805	advmod	as,as
R926	T1805	T1801	cc	as,with
R927	T1806	T1805	advmod	well,as
R928	T1738	T1734	appos	Isl,Lin
R929	T1807	T1801	conj	with,with
R93	T247	T248	det	the,pathway
R930	T1808	T1809	amod	nuclear,signaling
R931	T1739	T1738	punct	-,Isl
R932	T1809	T1807	pobj	signaling,with
R933	T1810	T1808	cc	and,nuclear
R934	T1811	T1808	conj	cytoplasmic,nuclear
R935	T1740	T1738	nummod	1,Isl
R936	T1812	T1792	punct	.,appear
R937	T1814	T1815	nsubj	This,provides
R938	T1741	T1734	punct	", ",Lin
R939	T1816	T1817	det	a,framework
R94	T248	T246	dobj	pathway,activate
R940	T1742	T1734	appos	Mec,Lin
R941	T1817	T1815	dobj	framework,provides
R942	T1818	T1817	prep	for,framework
R943	T1819	T1818	pcomp	conceptualizing,for
R944	T1820	T1821	advmod	why,appear
R945	T1821	T1819	ccomp	appear,conceptualizing
R946	T1822	T1823	compound	E,cadherin
R947	T1743	T1742	punct	-,Mec
R948	T1823	T1825	compound	cadherin,levels
R949	T1824	T1823	punct	-,cadherin
R95	T249	T250	nmod	Ras,kinase
R950	T1825	T1821	nsubj	levels,appear
R951	T1826	T1827	aux	to,impact
R952	T1744	T1742	nummod	3,Mec
R953	T1827	T1821	xcomp	impact,appear
R954	T1828	T1827	prep	upon,impact
R955	T1745	T1734	punct	(,Lin
R956	T1829	T1830	det	a,plethora
R957	T1830	T1828	pobj	plethora,upon
R958	T1831	T1830	prep	of,plethora
R959	T1746	T1734	appos	LIM,Lin
R96	T250	T248	nmod	kinase,pathway
R960	T1832	T1833	amod	developmental,processes
R961	T1833	T1831	pobj	processes,of
R962	T1834	T1835	punct	(,reviewed
R963	T1747	T1731	punct	),protein
R964	T1835	T1815	parataxis	reviewed,provides
R965	T1836	T1835	prep	in,reviewed
R966	T1837	T1836	punct	[,in
R967	T1748	T1731	appos	Ajuba,protein
R968	T1838	T1836	pobj	11,in
R969	T1839	T1835	punct	],reviewed
R97	T251	T250	punct	-,kinase
R970	T1840	T1835	punct	),reviewed
R971	T1749	T1731	punct	(,protein
R972	T1841	T1815	punct	.,provides
R973	T1750	T1751	det	a,member
R974	T1843	T1844	mark	As,probed
R975	T1844	T1846	advcl	probed,intrigued
R976	T1845	T1844	nsubj	we,probed
R977	T1751	T1731	appos	member,protein
R978	T1847	T1848	advmod	more,deeply
R979	T1848	T1844	advmod	deeply,probed
R98	T252	T253	npadvmod	mitogen,activated
R980	T1752	T1751	prep	of,member
R981	T1849	T1844	prep	into,probed
R982	T1850	T1851	det	the,mechanisms
R983	T1851	T1849	pobj	mechanisms,into
R984	T1753	T1754	det	the,family
R985	T1852	T1851	amod	underlying,mechanisms
R986	T1853	T1851	acl	governing,mechanisms
R987	T1854	T1855	compound	E,cadherin
R988	T1754	T1752	pobj	family,of
R989	T1855	T1857	compound	cadherin,promoter
R99	T253	T250	amod	activated,kinase
R990	T1856	T1855	punct	-,cadherin
R991	T1857	T1858	compound	promoter,activity
R992	T1755	T1754	compound	zyxin,family
R993	T1858	T1853	dobj	activity,governing
R994	T1859	T1846	punct	", ",intrigued
R995	T1756	T1754	prep	of,family
R996	T1860	T1846	nsubjpass	we,intrigued
R997	T1861	T1846	auxpass	were,intrigued
R998	T1862	T1846	agent	by,intrigued
R999	T1863	T1864	det	the,proximity

PMC:539061 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

PMC:539061 JSON TXT 8 Projects