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PMC:529315 / 34596-35397 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T19120 0-6 JJ denotes Spinal
T19121 7-12 NNS denotes cords
T19123 13-17 IN denotes with
T19124 18-26 VBN denotes attached
T19125 27-31 NNS denotes DRGs
T19126 32-36 VBD denotes were
T19122 37-46 VBN denotes dissected
T19127 47-50 RP denotes out
T19128 51-55 IN denotes from
T19129 56-61 NNP denotes Swiss
T19130 62-69 NNP denotes Webster
T19132 70-75 NN denotes mouse
T19131 76-83 NNS denotes embryos
T19133 84-86 IN denotes at
T19134 87-90 NN denotes E13
T19135 90-92 , denotes ,
T19136 92-95 CC denotes and
T19137 96-105 VBN denotes sectioned
T19138 106-110 IN denotes into
T19139 111-114 CD denotes 300
T19141 114-115 HYPH denotes -
T19140 115-117 NN denotes μm
T19143 117-118 HYPH denotes -
T19142 118-123 JJ denotes thick
T19144 124-130 NNS denotes slices
T19145 130-131 . denotes .
T19146 131-246 sentence denotes Tissue slices were placed on Millicell Tissue Culture Inserts (Millipore, Billrica, Massachusetts, United States).
T19147 132-138 NN denotes Tissue
T19148 139-145 NNS denotes slices
T19150 146-150 VBD denotes were
T19149 151-157 VBN denotes placed
T19151 158-160 IN denotes on
T19152 161-170 NN denotes Millicell
T19154 171-177 NN denotes Tissue
T19155 178-185 NN denotes Culture
T19153 186-193 NNS denotes Inserts
T19156 194-195 -LRB- denotes (
T19157 195-204 NNP denotes Millipore
T19158 204-206 , denotes ,
T19159 206-214 NNP denotes Billrica
T19160 214-216 , denotes ,
T19161 216-229 NNP denotes Massachusetts
T19162 229-231 , denotes ,
T19163 231-237 NNP denotes United
T19164 238-244 NNP denotes States
T19165 244-245 -RRB- denotes )
T19166 245-246 . denotes .
T19167 246-391 sentence denotes NT-3-loaded (n = 15) or PBS-loaded (n = 6) sepharose beads were prepared as described above, and placed in the midline at mid-spinal cord level.
T19168 247-249 NN denotes NT
T19170 249-250 HYPH denotes -
T19171 250-251 CD denotes 3
T19172 251-252 HYPH denotes -
T19169 252-258 VBN denotes loaded
T19174 259-260 -LRB- denotes (
T19176 260-261 NN denotes n
T19177 262-263 SYM denotes =
T19175 264-266 CD denotes 15
T19178 266-267 -RRB- denotes )
T19179 268-270 CC denotes or
T19180 271-274 NN denotes PBS
T19182 274-275 HYPH denotes -
T19181 275-281 VBN denotes loaded
T19183 282-283 -LRB- denotes (
T19185 283-284 NN denotes n
T19186 285-286 SYM denotes =
T19184 287-288 CD denotes 6
T19187 288-289 -RRB- denotes )
T19188 290-299 NN denotes sepharose
T19173 300-305 NNS denotes beads
T19190 306-310 VBD denotes were
T19189 311-319 VBN denotes prepared
T19191 320-322 IN denotes as
T19192 323-332 VBN denotes described
T19193 333-338 RB denotes above
T19194 338-340 , denotes ,
T19195 340-343 CC denotes and
T19196 344-350 VBN denotes placed
T19197 351-353 IN denotes in
T19198 354-357 DT denotes the
T19199 358-365 NN denotes midline
T19200 366-368 IN denotes at
T19201 369-379 JJ denotes mid-spinal
T19202 380-384 NN denotes cord
T19203 385-390 NN denotes level
T19204 390-391 . denotes .
T19205 391-534 sentence denotes Inserts were placed in six-well plates with serum-free medium at the bottom of the wells, and kept at 33 °C for 3 d in the presence of 5% CO2.
T19206 392-399 NNS denotes Inserts
T19208 400-404 VBD denotes were
T19207 405-411 VBN denotes placed
T19209 412-414 IN denotes in
T19210 415-418 CD denotes six
T19212 418-419 HYPH denotes -
T19211 419-423 NN denotes well
T19213 424-430 NNS denotes plates
T19214 431-435 IN denotes with
T19215 436-441 NN denotes serum
T19217 441-442 HYPH denotes -
T19216 442-446 JJ denotes free
T19218 447-453 NN denotes medium
T19219 454-456 IN denotes at
T19220 457-460 DT denotes the
T19221 461-467 NN denotes bottom
T19222 468-470 IN denotes of
T19223 471-474 DT denotes the
T19224 475-480 NNS denotes wells
T19225 480-482 , denotes ,
T19226 482-485 CC denotes and
T19227 486-490 VBD denotes kept
T19228 491-493 IN denotes at
T19229 494-496 CD denotes 33
T19230 497-499 NN denotes °C
T19231 500-503 IN denotes for
T19232 504-505 CD denotes 3
T19233 506-507 NN denotes d
T19234 508-510 IN denotes in
T19235 511-514 DT denotes the
T19236 515-523 NN denotes presence
T19237 524-526 IN denotes of
T19238 527-528 CD denotes 5
T19239 528-529 NN denotes %
T19240 530-533 NN denotes CO2
T19241 533-534 . denotes .
T19242 534-645 sentence denotes Cultures were then fixed with 4% paraformaldehyde in PBS, and small crystals of DiI were placed into the DRGs.
T19243 535-543 NNS denotes Cultures
T19245 544-548 VBD denotes were
T19246 549-553 RB denotes then
T19244 554-559 VBN denotes fixed
T19247 560-564 IN denotes with
T19248 565-566 CD denotes 4
T19249 566-567 NN denotes %
T19250 568-584 NN denotes paraformaldehyde
T19251 585-587 IN denotes in
T19252 588-591 NN denotes PBS
T19253 591-593 , denotes ,
T19254 593-596 CC denotes and
T19255 597-602 JJ denotes small
T19256 603-611 NNS denotes crystals
T19258 612-614 IN denotes of
T19259 615-618 NN denotes DiI
T19260 619-623 VBD denotes were
T19257 624-630 VBN denotes placed
T19261 631-635 IN denotes into
T19262 636-639 DT denotes the
T19263 640-644 NNS denotes DRGs
T19264 644-645 . denotes .
T19265 645-801 sentence denotes Samples were incubated in a 37-°C incubator, allowing the dye to diffuse, and photographed under a Nikon (Tokyo, Japan) inverted epifluoresence microscope.
T19266 646-653 NNS denotes Samples
T19268 654-658 VBD denotes were
T19267 659-668 VBN denotes incubated
T19269 669-671 IN denotes in
T19270 672-673 DT denotes a
T19272 674-676 CD denotes 37
T19274 676-677 HYPH denotes -
T19273 677-679 NN denotes °C
T19271 680-689 NN denotes incubator
T19275 689-691 , denotes ,
T19276 691-699 VBG denotes allowing
T19277 700-703 DT denotes the
T19278 704-707 NN denotes dye
T19280 708-710 TO denotes to
T19279 711-718 VB denotes diffuse
T19281 718-720 , denotes ,
T19282 720-723 CC denotes and
T19283 724-736 VBN denotes photographed
T19284 737-742 IN denotes under
T19285 743-744 DT denotes a
T19287 745-750 NNP denotes Nikon
T19288 751-752 -LRB- denotes (
T19289 752-757 NNP denotes Tokyo
T19290 757-759 , denotes ,
T19291 759-764 NNP denotes Japan
T19292 764-765 -RRB- denotes )
T19293 766-774 JJ denotes inverted
T19294 775-789 NN denotes epifluoresence
T19286 790-800 NN denotes microscope
T19295 800-801 . denotes .
R5635 T19120 T19121 amod Spinal,cords
R5636 T19121 T19122 nsubjpass cords,dissected
R5637 T19123 T19121 prep with,cords
R5638 T19124 T19125 amod attached,DRGs
R5639 T19125 T19123 pobj DRGs,with
R5640 T19126 T19122 auxpass were,dissected
R5641 T19127 T19122 prt out,dissected
R5642 T19128 T19122 prep from,dissected
R5643 T19129 T19130 compound Swiss,Webster
R5644 T19130 T19131 compound Webster,embryos
R5645 T19131 T19128 pobj embryos,from
R5646 T19132 T19131 compound mouse,embryos
R5647 T19133 T19131 prep at,embryos
R5648 T19134 T19133 pobj E13,at
R5649 T19135 T19122 punct ", ",dissected
R5650 T19136 T19122 cc and,dissected
R5651 T19137 T19122 conj sectioned,dissected
R5652 T19138 T19137 prep into,sectioned
R5653 T19139 T19140 nummod 300,μm
R5654 T19140 T19142 npadvmod μm,thick
R5655 T19141 T19140 punct -,μm
R5656 T19142 T19144 amod thick,slices
R5657 T19143 T19142 punct -,thick
R5658 T19144 T19138 pobj slices,into
R5659 T19145 T19122 punct .,dissected
R5660 T19147 T19148 compound Tissue,slices
R5661 T19148 T19149 nsubjpass slices,placed
R5662 T19150 T19149 auxpass were,placed
R5663 T19151 T19149 prep on,placed
R5664 T19152 T19153 compound Millicell,Inserts
R5665 T19153 T19151 pobj Inserts,on
R5666 T19154 T19155 compound Tissue,Culture
R5667 T19155 T19153 compound Culture,Inserts
R5668 T19156 T19157 punct (,Millipore
R5669 T19157 T19153 parataxis Millipore,Inserts
R5670 T19158 T19157 punct ", ",Millipore
R5671 T19159 T19157 npadvmod Billrica,Millipore
R5672 T19160 T19157 punct ", ",Millipore
R5673 T19161 T19157 npadvmod Massachusetts,Millipore
R5674 T19162 T19157 punct ", ",Millipore
R5675 T19163 T19164 compound United,States
R5676 T19164 T19157 npadvmod States,Millipore
R5677 T19165 T19157 punct ),Millipore
R5678 T19166 T19149 punct .,placed
R5679 T19168 T19169 npadvmod NT,loaded
R5680 T19169 T19173 amod loaded,beads
R5681 T19170 T19168 punct -,NT
R5682 T19171 T19168 nummod 3,NT
R5683 T19172 T19169 punct -,loaded
R5684 T19173 T19189 nsubjpass beads,prepared
R5685 T19174 T19175 punct (,15
R5686 T19175 T19169 parataxis 15,loaded
R5687 T19176 T19175 nsubj n,15
R5688 T19177 T19175 punct =,15
R5689 T19178 T19175 punct ),15
R5690 T19179 T19169 cc or,loaded
R5691 T19180 T19181 npadvmod PBS,loaded
R5692 T19181 T19169 conj loaded,loaded
R5693 T19182 T19181 punct -,loaded
R5694 T19183 T19184 punct (,6
R5695 T19184 T19181 parataxis 6,loaded
R5696 T19185 T19184 nsubj n,6
R5697 T19186 T19184 punct =,6
R5698 T19187 T19184 punct ),6
R5699 T19188 T19173 compound sepharose,beads
R5700 T19190 T19189 auxpass were,prepared
R5701 T19191 T19192 mark as,described
R5702 T19192 T19189 advcl described,prepared
R5703 T19193 T19192 advmod above,described
R5704 T19194 T19189 punct ", ",prepared
R5705 T19195 T19189 cc and,prepared
R5706 T19196 T19189 conj placed,prepared
R5707 T19197 T19196 prep in,placed
R5708 T19198 T19199 det the,midline
R5709 T19199 T19197 pobj midline,in
R5710 T19200 T19196 prep at,placed
R5711 T19201 T19202 amod mid-spinal,cord
R5712 T19202 T19203 compound cord,level
R5713 T19203 T19200 pobj level,at
R5714 T19204 T19189 punct .,prepared
R5715 T19206 T19207 nsubjpass Inserts,placed
R5716 T19208 T19207 auxpass were,placed
R5717 T19209 T19207 prep in,placed
R5718 T19210 T19211 nummod six,well
R5719 T19211 T19213 compound well,plates
R5720 T19212 T19211 punct -,well
R5721 T19213 T19209 pobj plates,in
R5722 T19214 T19207 prep with,placed
R5723 T19215 T19216 npadvmod serum,free
R5724 T19216 T19218 amod free,medium
R5725 T19217 T19216 punct -,free
R5726 T19218 T19214 pobj medium,with
R5727 T19219 T19218 prep at,medium
R5728 T19220 T19221 det the,bottom
R5729 T19221 T19219 pobj bottom,at
R5730 T19222 T19221 prep of,bottom
R5731 T19223 T19224 det the,wells
R5732 T19224 T19222 pobj wells,of
R5733 T19225 T19207 punct ", ",placed
R5734 T19226 T19207 cc and,placed
R5735 T19227 T19207 conj kept,placed
R5736 T19228 T19227 prep at,kept
R5737 T19229 T19230 nummod 33,°C
R5738 T19230 T19228 pobj °C,at
R5739 T19231 T19227 prep for,kept
R5740 T19232 T19233 nummod 3,d
R5741 T19233 T19231 pobj d,for
R5742 T19234 T19227 prep in,kept
R5743 T19235 T19236 det the,presence
R5744 T19236 T19234 pobj presence,in
R5745 T19237 T19236 prep of,presence
R5746 T19238 T19239 nummod 5,%
R5747 T19239 T19240 compound %,CO2
R5748 T19240 T19237 pobj CO2,of
R5749 T19241 T19207 punct .,placed
R5750 T19243 T19244 nsubjpass Cultures,fixed
R5751 T19245 T19244 auxpass were,fixed
R5752 T19246 T19244 advmod then,fixed
R5753 T19247 T19244 prep with,fixed
R5754 T19248 T19249 nummod 4,%
R5755 T19249 T19250 compound %,paraformaldehyde
R5756 T19250 T19247 pobj paraformaldehyde,with
R5757 T19251 T19244 prep in,fixed
R5758 T19252 T19251 pobj PBS,in
R5759 T19253 T19244 punct ", ",fixed
R5760 T19254 T19244 cc and,fixed
R5761 T19255 T19256 amod small,crystals
R5762 T19256 T19257 nsubjpass crystals,placed
R5763 T19257 T19244 conj placed,fixed
R5764 T19258 T19256 prep of,crystals
R5765 T19259 T19258 pobj DiI,of
R5766 T19260 T19257 auxpass were,placed
R5767 T19261 T19257 prep into,placed
R5768 T19262 T19263 det the,DRGs
R5769 T19263 T19261 pobj DRGs,into
R5770 T19264 T19257 punct .,placed
R5771 T19266 T19267 nsubjpass Samples,incubated
R5772 T19268 T19267 auxpass were,incubated
R5773 T19269 T19267 prep in,incubated
R5774 T19270 T19271 det a,incubator
R5775 T19271 T19269 pobj incubator,in
R5776 T19272 T19273 nummod 37,°C
R5777 T19273 T19271 compound °C,incubator
R5778 T19274 T19273 punct -,°C
R5779 T19275 T19267 punct ", ",incubated
R5780 T19276 T19267 advcl allowing,incubated
R5781 T19277 T19278 det the,dye
R5782 T19278 T19279 nsubj dye,diffuse
R5783 T19279 T19276 ccomp diffuse,allowing
R5784 T19280 T19279 aux to,diffuse
R5785 T19281 T19267 punct ", ",incubated
R5786 T19282 T19267 cc and,incubated
R5787 T19283 T19267 conj photographed,incubated
R5788 T19284 T19283 prep under,photographed
R5789 T19285 T19286 det a,microscope
R5790 T19286 T19284 pobj microscope,under
R5791 T19287 T19286 nmod Nikon,microscope
R5792 T19288 T19289 punct (,Tokyo
R5793 T19289 T19287 parataxis Tokyo,Nikon
R5794 T19290 T19289 punct ", ",Tokyo
R5795 T19291 T19289 npadvmod Japan,Tokyo
R5796 T19292 T19289 punct ),Tokyo
R5797 T19293 T19294 amod inverted,epifluoresence
R5798 T19294 T19286 compound epifluoresence,microscope
R5799 T19295 T19267 punct .,incubated