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PMC:529315 / 29537-35397 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T16203 23-33 NN denotes Generation
T16204 34-36 IN denotes of
T16205 37-43 JJ denotes double
T16206 44-47 NNS denotes KOs
T16207 47-48 . denotes .
T16208 48-244 sentence denotes We crossed Bax KO females on a C57BL/6 background (Jackson Laboratory, Bar Harbor, Maine, United States) to NT-3 heterozygote males on a 129 Sv background to generate double heterozygote animals.
T16209 49-51 PRP denotes We
T16210 52-59 VBD denotes crossed
T16211 60-63 NN denotes Bax
T16212 64-66 NN denotes KO
T16213 67-74 NNS denotes females
T16214 75-77 IN denotes on
T16215 78-79 DT denotes a
T16217 80-85 NN denotes C57BL
T16218 85-86 HYPH denotes /
T16219 86-87 CD denotes 6
T16216 88-98 NN denotes background
T16220 99-100 -LRB- denotes (
T16222 100-107 NNP denotes Jackson
T16221 108-118 NNP denotes Laboratory
T16223 118-120 , denotes ,
T16224 120-123 NNP denotes Bar
T16225 124-130 NNP denotes Harbor
T16226 130-132 , denotes ,
T16227 132-137 NNP denotes Maine
T16228 137-139 , denotes ,
T16229 139-145 NNP denotes United
T16230 146-152 NNP denotes States
T16231 152-153 -RRB- denotes )
T16232 154-156 IN denotes to
T16233 157-159 NN denotes NT
T16235 159-160 HYPH denotes -
T16236 160-161 CD denotes 3
T16234 162-174 NN denotes heterozygote
T16237 175-180 NNS denotes males
T16238 181-183 IN denotes on
T16239 184-185 DT denotes a
T16241 186-189 CD denotes 129
T16242 190-192 NN denotes Sv
T16240 193-203 NN denotes background
T16243 204-206 TO denotes to
T16244 207-215 VB denotes generate
T16245 216-222 JJ denotes double
T16246 223-235 NN denotes heterozygote
T16247 236-243 NNS denotes animals
T16248 243-244 . denotes .
T16249 244-352 sentence denotes Progeny was genotyped with PCR, and animals heterozygous for both genes were bred to obtain the double KOs.
T16250 245-252 NN denotes Progeny
T16252 253-256 VBD denotes was
T16251 257-266 VBN denotes genotyped
T16253 267-271 IN denotes with
T16254 272-275 NN denotes PCR
T16255 275-277 , denotes ,
T16256 277-280 CC denotes and
T16257 281-288 NNS denotes animals
T16259 289-301 JJ denotes heterozygous
T16260 302-305 IN denotes for
T16261 306-310 DT denotes both
T16262 311-316 NNS denotes genes
T16263 317-321 VBD denotes were
T16258 322-326 VBN denotes bred
T16264 327-329 TO denotes to
T16265 330-336 VB denotes obtain
T16266 337-340 DT denotes the
T16268 341-347 JJ denotes double
T16267 348-351 NNS denotes KOs
T16269 351-352 . denotes .
T16270 352-495 sentence denotes Primers used for the Bax locus were R661, GTT GAC CAG AGT GGC GTA GG; R662, CCG CTT CCA TTG CTC AGC GG; and R663, GAG CTG ATC AGA ACC ATC ATG.
T16271 353-360 NNS denotes Primers
T16273 361-365 VBN denotes used
T16274 366-369 IN denotes for
T16275 370-373 DT denotes the
T16277 374-377 NN denotes Bax
T16276 378-383 NN denotes locus
T16272 384-388 VBD denotes were
T16278 389-393 NN denotes R661
T16279 393-395 , denotes ,
T16280 395-398 NN denotes GTT
T16282 399-402 NN denotes GAC
T16283 403-406 NN denotes CAG
T16284 407-410 NN denotes AGT
T16285 411-414 NN denotes GGC
T16286 415-418 NN denotes GTA
T16281 419-421 NN denotes GG
T16287 421-422 : denotes ;
T16288 423-427 NN denotes R662
T16289 427-429 , denotes ,
T16290 429-432 NN denotes CCG
T16292 433-436 NN denotes CTT
T16293 437-440 NN denotes CCA
T16294 441-444 NN denotes TTG
T16295 445-448 NN denotes CTC
T16296 449-452 NN denotes AGC
T16291 453-455 NN denotes GG
T16297 455-456 : denotes ;
T16298 457-460 CC denotes and
T16299 461-465 NN denotes R663
T16300 465-467 , denotes ,
T16301 467-470 NN denotes GAG
T16303 471-474 NN denotes CTG
T16304 475-478 NN denotes ATC
T16305 479-482 NN denotes AGA
T16306 483-486 NN denotes ACC
T16307 487-490 NN denotes ATC
T16302 491-494 NN denotes ATG
T16308 494-495 . denotes .
T16309 495-678 sentence denotes Primers used for the NT-3 locus were NT3A, CGT GGT GAG GTT CTA TTG GCT AC; NT3B, CAG AGC ACC CTG CCC AAA GCA GAG; NT3R, CCT TGA CAA TAC TGA ATG CC; and NEOF, GGG AAC TTC CTG ACT AGG.
T16310 496-503 NNS denotes Primers
T16312 504-508 VBN denotes used
T16313 509-512 IN denotes for
T16314 513-516 DT denotes the
T16316 517-519 NN denotes NT
T16317 519-520 HYPH denotes -
T16318 520-521 CD denotes 3
T16315 522-527 NN denotes locus
T16311 528-532 VBD denotes were
T16319 533-537 NN denotes NT3A
T16320 537-539 , denotes ,
T16321 539-542 NN denotes CGT
T16323 543-546 NN denotes GGT
T16324 547-550 NN denotes GAG
T16325 551-554 NN denotes GTT
T16326 555-558 NN denotes CTA
T16327 559-562 NN denotes TTG
T16328 563-566 NN denotes GCT
T16322 567-569 NN denotes AC
T16329 569-570 : denotes ;
T16330 571-575 NN denotes NT3B
T16331 575-577 , denotes ,
T16332 577-580 NN denotes CAG
T16334 581-584 NN denotes AGC
T16335 585-588 NN denotes ACC
T16336 589-592 NN denotes CTG
T16337 593-596 NN denotes CCC
T16338 597-600 NN denotes AAA
T16339 601-604 NN denotes GCA
T16333 605-608 NN denotes GAG
T16340 608-609 : denotes ;
T16341 610-614 NN denotes NT3R
T16342 614-616 , denotes ,
T16343 616-619 NN denotes CCT
T16345 620-623 NN denotes TGA
T16346 624-627 NN denotes CAA
T16347 628-631 NN denotes TAC
T16348 632-635 NN denotes TGA
T16349 636-639 NN denotes ATG
T16344 640-642 NN denotes CC
T16350 642-643 : denotes ;
T16351 644-647 CC denotes and
T16352 648-652 NN denotes NEOF
T16353 652-654 , denotes ,
T16354 654-657 NN denotes GGG
T16356 658-661 NN denotes AAC
T16357 662-665 NN denotes TTC
T16358 666-669 NN denotes CTG
T16359 670-673 NN denotes ACT
T16355 674-677 NN denotes AGG
T16360 677-678 . denotes .
T16361 678-737 sentence denotes WT, Bax KO, and NT-3 KO littermates were used as controls.
T16362 679-681 NN denotes WT
T16364 681-683 , denotes ,
T16365 683-686 NN denotes Bax
T16366 687-689 NN denotes KO
T16367 689-691 , denotes ,
T16368 691-694 CC denotes and
T16369 695-697 NN denotes NT
T16371 697-698 HYPH denotes -
T16372 698-699 CD denotes 3
T16370 700-702 NN denotes KO
T16363 703-714 NNS denotes littermates
T16374 715-719 VBD denotes were
T16373 720-724 VBN denotes used
T16375 725-727 IN denotes as
T16376 728-736 NNS denotes controls
T16377 736-737 . denotes .
T16378 737-818 sentence denotes A total of 11 Bax/NT-3 double KO mice were analyzed, of these nine were P0 pups.
T16379 738-739 DT denotes A
T16380 740-745 NN denotes total
T16382 746-748 IN denotes of
T16383 749-751 CD denotes 11
T16385 752-755 NN denotes Bax
T16387 755-756 HYPH denotes /
T16388 756-758 NN denotes NT
T16389 758-759 HYPH denotes -
T16390 759-760 CD denotes 3
T16391 761-767 JJ denotes double
T16386 768-770 NN denotes KO
T16384 771-775 NNS denotes mice
T16392 776-780 VBD denotes were
T16381 781-789 VBN denotes analyzed
T16394 789-791 , denotes ,
T16395 791-793 IN denotes of
T16396 794-799 DT denotes these
T16397 800-804 CD denotes nine
T16393 805-809 VBD denotes were
T16398 810-812 NN denotes P0
T16399 813-817 NNS denotes pups
T16400 817-818 . denotes .
T16401 818-894 sentence denotes The p75 colony on a 129 S1 background was received from Jackson Laboratory.
T16402 819-822 DT denotes The
T16404 823-826 NN denotes p75
T16403 827-833 NN denotes colony
T16406 834-836 IN denotes on
T16407 837-838 DT denotes a
T16409 839-842 CD denotes 129
T16410 843-845 NN denotes S1
T16408 846-856 NN denotes background
T16411 857-860 VBD denotes was
T16405 861-869 VBN denotes received
T16412 870-874 IN denotes from
T16413 875-882 NNP denotes Jackson
T16414 883-893 NNP denotes Laboratory
T16415 893-894 . denotes .
T16416 894-1140 sentence denotes We used tail DNA to genotype animals using the primers IMR0013, CTT GGG TGG AGA GGC TAT TC; IMR0014, AGG TGA GAT GAC AGG AGA TC (generic neo primers); IMR0710, TGT TAC GTT CTC TGA CGT GGT GAG; and IMR0711, TCA GCC CAG GGT GTG CAC TC (p75 locus).
T16417 895-897 PRP denotes We
T16418 898-902 VBD denotes used
T16419 903-907 NN denotes tail
T16420 908-911 NN denotes DNA
T16421 912-914 TO denotes to
T16422 915-923 VB denotes genotype
T16423 924-931 NNS denotes animals
T16424 932-937 VBG denotes using
T16425 938-941 DT denotes the
T16426 942-949 NNS denotes primers
T16427 950-957 NN denotes IMR0013
T16428 957-959 , denotes ,
T16429 959-962 NN denotes CTT
T16431 963-966 NN denotes GGG
T16432 967-970 NN denotes TGG
T16433 971-974 NN denotes AGA
T16434 975-978 NN denotes GGC
T16435 979-982 NN denotes TAT
T16430 983-985 NN denotes TC
T16436 985-986 : denotes ;
T16437 987-994 NN denotes IMR0014
T16438 994-996 , denotes ,
T16439 996-999 NN denotes AGG
T16441 1000-1003 NN denotes TGA
T16442 1004-1007 NN denotes GAT
T16443 1008-1011 NN denotes GAC
T16444 1012-1015 NN denotes AGG
T16445 1016-1019 NN denotes AGA
T16440 1020-1022 NN denotes TC
T16446 1023-1024 -LRB- denotes (
T16447 1024-1031 JJ denotes generic
T16449 1032-1035 NN denotes neo
T16448 1036-1043 NNS denotes primers
T16450 1043-1044 -RRB- denotes )
T16451 1044-1045 : denotes ;
T16452 1046-1053 NN denotes IMR0710
T16453 1053-1055 , denotes ,
T16454 1055-1058 NN denotes TGT
T16456 1059-1062 NN denotes TAC
T16457 1063-1066 NN denotes GTT
T16458 1067-1070 NN denotes CTC
T16459 1071-1074 NN denotes TGA
T16460 1075-1078 NN denotes CGT
T16461 1079-1082 NN denotes GGT
T16455 1083-1086 NN denotes GAG
T16462 1086-1087 : denotes ;
T16463 1088-1091 CC denotes and
T16464 1092-1099 NN denotes IMR0711
T16465 1099-1101 , denotes ,
T16466 1101-1104 NN denotes TCA
T16468 1105-1108 NN denotes GCC
T16469 1109-1112 NN denotes CAG
T16470 1113-1116 NN denotes GGT
T16471 1117-1120 NN denotes GTG
T16472 1121-1124 NN denotes CAC
T16467 1125-1127 NN denotes TC
T16473 1128-1129 -LRB- denotes (
T16475 1129-1132 NN denotes p75
T16474 1133-1138 NN denotes locus
T16476 1138-1139 -RRB- denotes )
T16477 1139-1140 . denotes .
T16478 1140-1209 sentence denotes For embryonic experiments, day of plug positivity was considered E0.
T16479 1141-1144 IN denotes For
T16481 1145-1154 JJ denotes embryonic
T16482 1155-1166 NNS denotes experiments
T16483 1166-1168 , denotes ,
T16484 1168-1171 NN denotes day
T16485 1172-1174 IN denotes of
T16486 1175-1179 NN denotes plug
T16487 1180-1190 NN denotes positivity
T16488 1191-1194 VBD denotes was
T16480 1195-1205 VBN denotes considered
T16489 1206-1208 NN denotes E0
T16490 1208-1209 . denotes .
T16491 1209-1459 sentence denotes All of the protocols used in this study were approved by the Louisiana State University Health Sciences Center Institutional Animal Care and Use Committee and conformed to the National Institutes of Health guidelines for use of experimental animals.
T16492 1210-1213 DT denotes All
T16494 1214-1216 IN denotes of
T16495 1217-1220 DT denotes the
T16496 1221-1230 NNS denotes protocols
T16497 1231-1235 VBN denotes used
T16498 1236-1238 IN denotes in
T16499 1239-1243 DT denotes this
T16500 1244-1249 NN denotes study
T16501 1250-1254 VBD denotes were
T16493 1255-1263 VBN denotes approved
T16502 1264-1266 IN denotes by
T16503 1267-1270 DT denotes the
T16505 1271-1280 NNP denotes Louisiana
T16507 1281-1286 NNP denotes State
T16506 1287-1297 NNP denotes University
T16509 1298-1304 NNP denotes Health
T16510 1305-1313 NNPS denotes Sciences
T16508 1314-1320 NNP denotes Center
T16511 1321-1334 NNP denotes Institutional
T16512 1335-1341 NNP denotes Animal
T16513 1342-1346 NNP denotes Care
T16514 1347-1350 CC denotes and
T16515 1351-1354 NNP denotes Use
T16504 1355-1364 NNP denotes Committee
T16516 1365-1368 CC denotes and
T16517 1369-1378 VBD denotes conformed
T16518 1379-1381 IN denotes to
T16519 1382-1385 DT denotes the
T16521 1386-1394 NNP denotes National
T16522 1395-1405 NNPS denotes Institutes
T16523 1406-1408 IN denotes of
T16524 1409-1415 NNP denotes Health
T16520 1416-1426 NNS denotes guidelines
T16525 1427-1430 IN denotes for
T16526 1431-1434 NN denotes use
T16527 1435-1437 IN denotes of
T16528 1438-1450 JJ denotes experimental
T16529 1451-1458 NNS denotes animals
T16530 1458-1459 . denotes .
T16880 1461-1465 NN denotes TrkA
T16882 1465-1466 HYPH denotes /
T16881 1466-1470 NN denotes TrkC
T16883 1471-1491 NN denotes immunohistochemistry
T16884 1491-1492 . denotes .
T16885 1492-1890 sentence denotes Frozen spinal cord sections (10 μm thick) were blocked and incubated in a cocktail of rabbit anti-TrkA and goat anti-TrkC antibodies (gift of Dr. Reichardt; Huang et al. 1999), followed by a cocktail of CY3 conjugated donkey anti-rabbit and FITC conjugated donkey anti-goat antibodies (Chemicon, Temecula, California, United States) in the presence of 0.3% TritonX-100 and 10% normal donkey serum.
T16886 1493-1499 JJ denotes Frozen
T16888 1500-1506 JJ denotes spinal
T16889 1507-1511 NN denotes cord
T16887 1512-1520 NNS denotes sections
T16891 1521-1522 -LRB- denotes (
T16892 1522-1524 CD denotes 10
T16893 1525-1527 NN denotes μm
T16894 1528-1533 JJ denotes thick
T16895 1533-1534 -RRB- denotes )
T16896 1535-1539 VBD denotes were
T16890 1540-1547 VBN denotes blocked
T16897 1548-1551 CC denotes and
T16898 1552-1561 VBN denotes incubated
T16899 1562-1564 IN denotes in
T16900 1565-1566 DT denotes a
T16901 1567-1575 NN denotes cocktail
T16902 1576-1578 IN denotes of
T16903 1579-1585 NN denotes rabbit
T16904 1586-1595 JJ denotes anti-TrkA
T16905 1596-1599 CC denotes and
T16906 1600-1604 NN denotes goat
T16908 1605-1614 JJ denotes anti-TrkC
T16907 1615-1625 NNS denotes antibodies
T16909 1626-1627 -LRB- denotes (
T16910 1627-1631 NN denotes gift
T16911 1632-1634 IN denotes of
T16912 1635-1638 NNP denotes Dr.
T16913 1639-1648 NNP denotes Reichardt
T16914 1648-1649 : denotes ;
T16915 1650-1655 NNP denotes Huang
T16916 1656-1658 FW denotes et
T16917 1659-1662 FW denotes al.
T16918 1663-1667 CD denotes 1999
T16919 1667-1668 -RRB- denotes )
T16920 1668-1670 , denotes ,
T16921 1670-1678 VBN denotes followed
T16922 1679-1681 IN denotes by
T16923 1682-1683 DT denotes a
T16924 1684-1692 NN denotes cocktail
T16925 1693-1695 IN denotes of
T16926 1696-1699 NN denotes CY3
T16927 1700-1710 VBN denotes conjugated
T16928 1711-1717 NN denotes donkey
T16929 1718-1729 JJ denotes anti-rabbit
T16930 1730-1733 CC denotes and
T16931 1734-1738 NN denotes FITC
T16932 1739-1749 VBN denotes conjugated
T16933 1750-1756 NN denotes donkey
T16935 1757-1766 JJ denotes anti-goat
T16934 1767-1777 NNS denotes antibodies
T16936 1778-1779 -LRB- denotes (
T16937 1779-1787 NNP denotes Chemicon
T16938 1787-1789 , denotes ,
T16939 1789-1797 NNP denotes Temecula
T16940 1797-1799 , denotes ,
T16941 1799-1809 NNP denotes California
T16942 1809-1811 , denotes ,
T16943 1811-1817 NNP denotes United
T16944 1818-1824 NNP denotes States
T16945 1824-1825 -RRB- denotes )
T16946 1826-1828 IN denotes in
T16947 1829-1832 DT denotes the
T16948 1833-1841 NN denotes presence
T16949 1842-1844 IN denotes of
T16950 1845-1848 CD denotes 0.3
T16951 1848-1849 NN denotes %
T16952 1850-1857 NN denotes TritonX
T16953 1857-1858 HYPH denotes -
T16954 1858-1861 CD denotes 100
T16955 1862-1865 CC denotes and
T16956 1866-1868 CD denotes 10
T16957 1868-1869 NN denotes %
T16959 1870-1876 JJ denotes normal
T16960 1877-1883 NN denotes donkey
T16958 1884-1889 NN denotes serum
T16961 1889-1890 . denotes .
T16962 1890-2142 sentence denotes For PV immunohistochemistry, sections were reacted with monoclonal mouse anti-PV antibody (Sigma, St. Louis, Missouri, United States), and developed by Vector fluorescein mouse on mouse kit (Vector Laboratories, Burlingame, California, United States).
T16963 1891-1894 IN denotes For
T16965 1895-1897 NN denotes PV
T16966 1898-1918 NN denotes immunohistochemistry
T16967 1918-1920 , denotes ,
T16968 1920-1928 NNS denotes sections
T16969 1929-1933 VBD denotes were
T16964 1934-1941 VBN denotes reacted
T16970 1942-1946 IN denotes with
T16971 1947-1957 JJ denotes monoclonal
T16973 1958-1963 NN denotes mouse
T16974 1964-1971 JJ denotes anti-PV
T16972 1972-1980 NN denotes antibody
T16975 1981-1982 -LRB- denotes (
T16976 1982-1987 NNP denotes Sigma
T16977 1987-1989 , denotes ,
T16978 1989-1992 NNP denotes St.
T16979 1993-1998 NNP denotes Louis
T16980 1998-2000 , denotes ,
T16981 2000-2008 NNP denotes Missouri
T16982 2008-2010 , denotes ,
T16983 2010-2016 NNP denotes United
T16984 2017-2023 NNP denotes States
T16985 2023-2024 -RRB- denotes )
T16986 2024-2026 , denotes ,
T16987 2026-2029 CC denotes and
T16988 2030-2039 VBN denotes developed
T16989 2040-2042 IN denotes by
T16990 2043-2049 NNP denotes Vector
T16991 2050-2061 NN denotes fluorescein
T16993 2062-2067 NN denotes mouse
T16994 2068-2070 IN denotes on
T16995 2071-2076 NN denotes mouse
T16992 2077-2080 NN denotes kit
T16996 2081-2082 -LRB- denotes (
T16998 2082-2088 NNP denotes Vector
T16997 2089-2101 NNP denotes Laboratories
T16999 2101-2103 , denotes ,
T17000 2103-2113 NNP denotes Burlingame
T17001 2113-2115 , denotes ,
T17002 2115-2125 NNP denotes California
T17003 2125-2127 , denotes ,
T17004 2127-2133 NNP denotes United
T17005 2134-2140 NNP denotes States
T17006 2140-2141 -RRB- denotes )
T17007 2141-2142 . denotes .
T17008 2142-2292 sentence denotes For quantification purposes, TrkA- and TrkC-labeled cells from four different DRGs of each genotype and age studied were counted, and ratios plotted.
T17009 2143-2146 IN denotes For
T17011 2147-2161 NN denotes quantification
T17012 2162-2170 NNS denotes purposes
T17013 2170-2172 , denotes ,
T17014 2172-2176 NN denotes TrkA
T17016 2176-2177 HYPH denotes -
T17017 2178-2181 CC denotes and
T17018 2182-2186 NN denotes TrkC
T17019 2186-2187 HYPH denotes -
T17015 2187-2194 VBN denotes labeled
T17020 2195-2200 NNS denotes cells
T17021 2201-2205 IN denotes from
T17022 2206-2210 CD denotes four
T17024 2211-2220 JJ denotes different
T17023 2221-2225 NNS denotes DRGs
T17025 2226-2228 IN denotes of
T17026 2229-2233 DT denotes each
T17027 2234-2242 NN denotes genotype
T17028 2243-2246 CC denotes and
T17029 2247-2250 NN denotes age
T17030 2251-2258 VBN denotes studied
T17031 2259-2263 VBD denotes were
T17010 2264-2271 VBN denotes counted
T17032 2271-2273 , denotes ,
T17033 2273-2276 CC denotes and
T17034 2277-2283 NNS denotes ratios
T17035 2284-2291 VBN denotes plotted
T17036 2291-2292 . denotes .
T17352 2294-2300 NN denotes Muscle
T17353 2301-2308 NN denotes spindle
T17354 2309-2318 NN denotes detection
T17355 2318-2319 . denotes .
T17356 2319-2519 sentence denotes Gastrocnemius muscle was dissected out in P0 pups and sectioned longitudinally, or, in some cases, the whole leg at the level of gastrocnemius muscle with tibial nerve was sectioned in cross section.
T17357 2320-2333 NN denotes Gastrocnemius
T17358 2334-2340 NN denotes muscle
T17360 2341-2344 VBD denotes was
T17359 2345-2354 VBN denotes dissected
T17361 2355-2358 RP denotes out
T17362 2359-2361 IN denotes in
T17363 2362-2364 NN denotes P0
T17364 2365-2369 NNS denotes pups
T17365 2370-2373 CC denotes and
T17366 2374-2383 VBN denotes sectioned
T17367 2384-2398 RB denotes longitudinally
T17368 2398-2400 , denotes ,
T17369 2400-2402 CC denotes or
T17370 2402-2404 , denotes ,
T17371 2404-2406 IN denotes in
T17373 2407-2411 DT denotes some
T17374 2412-2417 NNS denotes cases
T17375 2417-2419 , denotes ,
T17376 2419-2422 DT denotes the
T17378 2423-2428 JJ denotes whole
T17377 2429-2432 NN denotes leg
T17379 2433-2435 IN denotes at
T17380 2436-2439 DT denotes the
T17381 2440-2445 NN denotes level
T17382 2446-2448 IN denotes of
T17383 2449-2462 NN denotes gastrocnemius
T17384 2463-2469 NN denotes muscle
T17385 2470-2474 IN denotes with
T17386 2475-2481 JJ denotes tibial
T17387 2482-2487 NN denotes nerve
T17388 2488-2491 VBD denotes was
T17372 2492-2501 VBN denotes sectioned
T17389 2502-2504 IN denotes in
T17390 2505-2510 NN denotes cross
T17391 2511-2518 NN denotes section
T17392 2518-2519 . denotes .
T17393 2519-2877 sentence denotes Sections were incubated with monoclonal S46 antibody (gift of Dr. Stockdale) reactive to the spindle-specific slow-tonic myosin heavy chain isoform and developed by Vector mouse on mouse kit as described above, followed by rabbit anti-NF-M antibody (Chemicon) and CY3 conjugated goat anti-rabbit antibody (Chemicon) in a sequential double-labeling protocol.
T17394 2520-2528 NNS denotes Sections
T17396 2529-2533 VBD denotes were
T17395 2534-2543 VBN denotes incubated
T17397 2544-2548 IN denotes with
T17398 2549-2559 JJ denotes monoclonal
T17400 2560-2563 NN denotes S46
T17399 2564-2572 NN denotes antibody
T17401 2573-2574 -LRB- denotes (
T17402 2574-2578 NN denotes gift
T17403 2579-2581 IN denotes of
T17404 2582-2585 NNP denotes Dr.
T17405 2586-2595 NNP denotes Stockdale
T17406 2595-2596 -RRB- denotes )
T17407 2597-2605 JJ denotes reactive
T17408 2606-2608 IN denotes to
T17409 2609-2612 DT denotes the
T17411 2613-2620 NN denotes spindle
T17413 2620-2621 HYPH denotes -
T17412 2621-2629 JJ denotes specific
T17414 2630-2634 JJ denotes slow
T17416 2634-2635 HYPH denotes -
T17415 2635-2640 NN denotes tonic
T17417 2641-2647 NN denotes myosin
T17418 2648-2653 JJ denotes heavy
T17419 2654-2659 NN denotes chain
T17410 2660-2667 NN denotes isoform
T17420 2668-2671 CC denotes and
T17421 2672-2681 VBN denotes developed
T17422 2682-2684 IN denotes by
T17423 2685-2691 NNP denotes Vector
T17425 2692-2697 NN denotes mouse
T17426 2698-2700 IN denotes on
T17427 2701-2706 NN denotes mouse
T17424 2707-2710 NN denotes kit
T17428 2711-2713 IN denotes as
T17429 2714-2723 VBN denotes described
T17430 2724-2729 RB denotes above
T17431 2729-2731 , denotes ,
T17432 2731-2739 VBN denotes followed
T17433 2740-2742 IN denotes by
T17434 2743-2749 NN denotes rabbit
T17436 2750-2757 JJ denotes anti-NF
T17438 2757-2758 HYPH denotes -
T17437 2758-2759 NN denotes M
T17435 2760-2768 NN denotes antibody
T17439 2769-2770 -LRB- denotes (
T17440 2770-2778 NNP denotes Chemicon
T17441 2778-2779 -RRB- denotes )
T17442 2780-2783 CC denotes and
T17443 2784-2787 NN denotes CY3
T17445 2788-2798 VBN denotes conjugated
T17446 2799-2803 NN denotes goat
T17447 2804-2815 JJ denotes anti-rabbit
T17444 2816-2824 NN denotes antibody
T17448 2825-2826 -LRB- denotes (
T17449 2826-2834 NNP denotes Chemicon
T17450 2834-2835 -RRB- denotes )
T17451 2836-2838 IN denotes in
T17452 2839-2840 DT denotes a
T17454 2841-2851 JJ denotes sequential
T17455 2852-2858 JJ denotes double
T17457 2858-2859 HYPH denotes -
T17456 2859-2867 NN denotes labeling
T17453 2868-2876 NN denotes protocol
T17458 2876-2877 . denotes .
T17459 2877-3074 sentence denotes In another method, DiI crystals were placed in DRGs of E17 embryos from different genotypes (n = 2), and the gastrocnemius muscle was isolated and sectioned into 40-μm-thick slices on a vibratome.
T17460 2878-2880 IN denotes In
T17462 2881-2888 DT denotes another
T17463 2889-2895 NN denotes method
T17464 2895-2897 , denotes ,
T17465 2897-2900 NN denotes DiI
T17466 2901-2909 NNS denotes crystals
T17467 2910-2914 VBD denotes were
T17461 2915-2921 VBN denotes placed
T17468 2922-2924 IN denotes in
T17469 2925-2929 NNS denotes DRGs
T17470 2930-2932 IN denotes of
T17471 2933-2936 NN denotes E17
T17472 2937-2944 NNS denotes embryos
T17473 2945-2949 IN denotes from
T17474 2950-2959 JJ denotes different
T17475 2960-2969 NNS denotes genotypes
T17476 2970-2971 -LRB- denotes (
T17478 2971-2972 NN denotes n
T17479 2973-2974 SYM denotes =
T17477 2975-2976 CD denotes 2
T17480 2976-2977 -RRB- denotes )
T17481 2977-2979 , denotes ,
T17482 2979-2982 CC denotes and
T17483 2983-2986 DT denotes the
T17485 2987-3000 NN denotes gastrocnemius
T17484 3001-3007 NN denotes muscle
T17487 3008-3011 VBD denotes was
T17486 3012-3020 VBN denotes isolated
T17488 3021-3024 CC denotes and
T17489 3025-3034 VBN denotes sectioned
T17490 3035-3039 IN denotes into
T17491 3040-3042 CD denotes 40
T17493 3042-3043 HYPH denotes -
T17492 3043-3045 NN denotes μm
T17495 3045-3046 HYPH denotes -
T17494 3046-3051 JJ denotes thick
T17496 3052-3058 NNS denotes slices
T17497 3059-3061 IN denotes on
T17498 3062-3063 DT denotes a
T17499 3064-3073 NN denotes vibratome
T17500 3073-3074 . denotes .
T17771 3076-3079 NN denotes DiI
T17772 3080-3088 NN denotes labeling
T17773 3088-3089 . denotes .
T17774 3089-3366 sentence denotes Spinal cords were dissected out with the DRGs attached, motor root was cut to prevent backfilling of motor neurons, and crystals of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI; Molecular Probes, Eugene, Oregon, United States) were inserted in DRGs.
T17775 3090-3096 JJ denotes Spinal
T17776 3097-3102 NNS denotes cords
T17778 3103-3107 VBD denotes were
T17777 3108-3117 VBN denotes dissected
T17779 3118-3121 RP denotes out
T17780 3122-3126 IN denotes with
T17782 3127-3130 DT denotes the
T17783 3131-3135 NNS denotes DRGs
T17781 3136-3144 VBN denotes attached
T17784 3144-3146 , denotes ,
T17785 3146-3151 NN denotes motor
T17786 3152-3156 NN denotes root
T17788 3157-3160 VBD denotes was
T17787 3161-3164 VBN denotes cut
T17789 3165-3167 TO denotes to
T17790 3168-3175 VB denotes prevent
T17791 3176-3187 NN denotes backfilling
T17792 3188-3190 IN denotes of
T17793 3191-3196 NN denotes motor
T17794 3197-3204 NNS denotes neurons
T17795 3204-3206 , denotes ,
T17796 3206-3209 CC denotes and
T17797 3210-3218 NNS denotes crystals
T17799 3219-3221 IN denotes of
T17800 3222-3223 CD denotes 1
T17802 3223-3224 , denotes ,
T17803 3224-3225 CD denotes 1
T17804 3225-3226 SYM denotes ′
T17805 3226-3227 HYPH denotes -
T17806 3227-3238 NN denotes dioctadecyl
T17807 3238-3239 HYPH denotes -
T17808 3239-3240 CD denotes 3
T17809 3240-3241 , denotes ,
T17810 3241-3242 CD denotes 3
T17811 3242-3243 , denotes ,
T17812 3243-3244 CD denotes 3
T17813 3244-3245 SYM denotes ′
T17814 3245-3246 , denotes ,
T17815 3246-3247 CD denotes 3
T17816 3247-3248 SYM denotes ′
T17817 3248-3249 HYPH denotes -
T17818 3249-3276 NN denotes tetramethylindocarbocyanine
T17801 3277-3288 NN denotes perchlorate
T17819 3289-3290 -LRB- denotes (
T17821 3290-3293 NN denotes DiI
T17822 3293-3294 : denotes ;
T17823 3295-3304 NNP denotes Molecular
T17820 3305-3311 NNP denotes Probes
T17824 3311-3313 , denotes ,
T17825 3313-3319 NNP denotes Eugene
T17826 3319-3321 , denotes ,
T17827 3321-3327 NNP denotes Oregon
T17828 3327-3329 , denotes ,
T17829 3329-3335 NNP denotes United
T17830 3336-3342 NNP denotes States
T17831 3342-3343 -RRB- denotes )
T17832 3344-3348 VBD denotes were
T17798 3349-3357 VBN denotes inserted
T17833 3358-3360 IN denotes in
T17834 3361-3365 NNS denotes DRGs
T17835 3365-3366 . denotes .
T17836 3366-3459 sentence denotes Labeled spinal cords were incubated at 37 °C for 8 d and sectioned on a vibrotome at 100 μm.
T17837 3367-3374 VBN denotes Labeled
T17839 3375-3381 JJ denotes spinal
T17838 3382-3387 NNS denotes cords
T17841 3388-3392 VBD denotes were
T17840 3393-3402 VBN denotes incubated
T17842 3403-3405 IN denotes at
T17843 3406-3408 CD denotes 37
T17844 3409-3411 NN denotes °C
T17845 3412-3415 IN denotes for
T17846 3416-3417 CD denotes 8
T17847 3418-3419 NN denotes d
T17848 3420-3423 CC denotes and
T17849 3424-3433 VBN denotes sectioned
T17850 3434-3436 IN denotes on
T17851 3437-3438 DT denotes a
T17852 3439-3448 NN denotes vibrotome
T17853 3449-3451 IN denotes at
T17854 3452-3455 CD denotes 100
T17855 3456-3458 NN denotes μm
T17856 3458-3459 . denotes .
T17857 3459-3633 sentence denotes Sections were observed under the fluorescent microscope and photographed, and images were transferred to Adobe Photoshop, inverted, and adjusted for brightness and contrast.
T17858 3460-3468 NNS denotes Sections
T17860 3469-3473 VBD denotes were
T17859 3474-3482 VBN denotes observed
T17861 3483-3488 IN denotes under
T17862 3489-3492 DT denotes the
T17864 3493-3504 JJ denotes fluorescent
T17863 3505-3515 NN denotes microscope
T17865 3516-3519 CC denotes and
T17866 3520-3532 VBN denotes photographed
T17867 3532-3534 , denotes ,
T17868 3534-3537 CC denotes and
T17869 3538-3544 NNS denotes images
T17871 3545-3549 VBD denotes were
T17870 3550-3561 VBN denotes transferred
T17872 3562-3564 IN denotes to
T17873 3565-3570 NNP denotes Adobe
T17874 3571-3580 NNP denotes Photoshop
T17875 3580-3582 , denotes ,
T17876 3582-3590 VBN denotes inverted
T17877 3590-3592 , denotes ,
T17878 3592-3595 CC denotes and
T17879 3596-3604 VBN denotes adjusted
T17880 3605-3608 IN denotes for
T17881 3609-3619 NN denotes brightness
T17882 3620-3623 CC denotes and
T17883 3624-3632 NN denotes contrast
T17884 3632-3633 . denotes .
T17885 3633-3722 sentence denotes Photoconverted in 0.15% DAB (3,3′-diaminobenzidine; Sigma) in 0.1M Tris buffer (pH 8.2).
T17886 3634-3648 VBN denotes Photoconverted
T17887 3649-3651 IN denotes in
T17888 3652-3656 CD denotes 0.15
T17889 3656-3657 NN denotes %
T17890 3658-3661 NN denotes DAB
T17891 3662-3663 -LRB- denotes (
T17893 3663-3664 CD denotes 3
T17895 3664-3665 , denotes ,
T17896 3665-3666 CD denotes 3
T17897 3666-3667 SYM denotes ′
T17898 3667-3668 HYPH denotes -
T17894 3668-3684 NN denotes diaminobenzidine
T17899 3684-3685 : denotes ;
T17892 3686-3691 NNP denotes Sigma
T17900 3691-3692 -RRB- denotes )
T17901 3693-3695 IN denotes in
T17902 3696-3699 CD denotes 0.1
T17903 3699-3700 NN denotes M
T17905 3701-3705 NN denotes Tris
T17904 3706-3712 NN denotes buffer
T17906 3713-3714 -LRB- denotes (
T17907 3714-3716 NN denotes pH
T17908 3717-3720 CD denotes 8.2
T17909 3720-3721 -RRB- denotes )
T17910 3721-3722 . denotes .
T18468 3724-3726 FW denotes In
T18469 3727-3732 FW denotes vitro
T18471 3733-3743 NN denotes co-culture
T18470 3744-3749 NN denotes assay
T18472 3749-3750 . denotes .
T18473 3750-3840 sentence denotes DRG explants were derived from Swiss Webster, Bax null, and p75 null mouse embryos (E13).
T18474 3751-3754 NN denotes DRG
T18475 3755-3763 NNS denotes explants
T18477 3764-3768 VBD denotes were
T18476 3769-3776 VBN denotes derived
T18478 3777-3781 IN denotes from
T18479 3782-3787 NNP denotes Swiss
T18480 3788-3795 NNP denotes Webster
T18482 3795-3797 , denotes ,
T18483 3797-3800 NNP denotes Bax
T18484 3801-3805 JJ denotes null
T18485 3805-3807 , denotes ,
T18486 3807-3810 CC denotes and
T18487 3811-3814 NN denotes p75
T18488 3815-3819 JJ denotes null
T18489 3820-3825 NN denotes mouse
T18481 3826-3833 NNS denotes embryos
T18490 3834-3835 -LRB- denotes (
T18491 3835-3838 NN denotes E13
T18492 3838-3839 -RRB- denotes )
T18493 3839-3840 . denotes .
T18494 3840-3941 sentence denotes For collagen matrix assays, DRGs were dissected out under a stereomicroscope using tungsten needles.
T18495 3841-3844 IN denotes For
T18497 3845-3853 NN denotes collagen
T18498 3854-3860 NN denotes matrix
T18499 3861-3867 NNS denotes assays
T18500 3867-3869 , denotes ,
T18501 3869-3873 NNS denotes DRGs
T18502 3874-3878 VBD denotes were
T18496 3879-3888 VBN denotes dissected
T18503 3889-3892 RP denotes out
T18504 3893-3898 IN denotes under
T18505 3899-3900 DT denotes a
T18506 3901-3917 NN denotes stereomicroscope
T18507 3918-3923 VBG denotes using
T18508 3924-3932 NN denotes tungsten
T18509 3933-3940 NNS denotes needles
T18510 3940-3941 . denotes .
T18511 3941-4127 sentence denotes Collagen matrix was prepared with 430 μl of collagen (3 mg/ml dissolved in 0.1M acetic acid, Sigma), 50 μl of 10X DMEM medium, and 2.5 μl of 0.8 M NaCa2, and the pH was adjusted to 7.5.
T18512 3942-3950 NN denotes Collagen
T18513 3951-3957 NN denotes matrix
T18515 3958-3961 VBD denotes was
T18514 3962-3970 VBN denotes prepared
T18516 3971-3975 IN denotes with
T18517 3976-3979 CD denotes 430
T18518 3980-3982 NN denotes μl
T18519 3983-3985 IN denotes of
T18520 3986-3994 NN denotes collagen
T18521 3995-3996 -LRB- denotes (
T18523 3996-3997 CD denotes 3
T18524 3998-4000 NN denotes mg
T18525 4000-4001 SYM denotes /
T18526 4001-4003 NN denotes ml
T18527 4004-4013 VBN denotes dissolved
T18528 4014-4016 IN denotes in
T18529 4017-4020 CD denotes 0.1
T18530 4020-4021 NN denotes M
T18532 4022-4028 JJ denotes acetic
T18531 4029-4033 NN denotes acid
T18533 4033-4035 , denotes ,
T18522 4035-4040 NNP denotes Sigma
T18534 4040-4041 -RRB- denotes )
T18535 4041-4043 , denotes ,
T18536 4043-4045 CD denotes 50
T18537 4046-4048 NN denotes μl
T18538 4049-4051 IN denotes of
T18539 4052-4054 CD denotes 10
T18541 4054-4055 NN denotes X
T18540 4056-4060 NN denotes DMEM
T18542 4061-4067 NN denotes medium
T18543 4067-4069 , denotes ,
T18544 4069-4072 CC denotes and
T18545 4073-4076 CD denotes 2.5
T18546 4077-4079 NN denotes μl
T18547 4080-4082 IN denotes of
T18548 4083-4086 CD denotes 0.8
T18549 4087-4088 NN denotes M
T18550 4089-4094 NN denotes NaCa2
T18551 4094-4096 , denotes ,
T18552 4096-4099 CC denotes and
T18553 4100-4103 DT denotes the
T18554 4104-4106 NN denotes pH
T18556 4107-4110 VBD denotes was
T18555 4111-4119 VBN denotes adjusted
T18557 4120-4122 IN denotes to
T18558 4123-4126 CD denotes 7.5
T18559 4126-4127 . denotes .
T18560 4127-4230 sentence denotes Individual ganglion explants were placed in 24-well plates and covered with freshly prepared collagen.
T18561 4128-4138 JJ denotes Individual
T18563 4139-4147 NN denotes ganglion
T18562 4148-4156 NNS denotes explants
T18565 4157-4161 VBD denotes were
T18564 4162-4168 VBN denotes placed
T18566 4169-4171 IN denotes in
T18567 4172-4174 CD denotes 24
T18569 4174-4175 HYPH denotes -
T18568 4175-4179 NN denotes well
T18570 4180-4186 NNS denotes plates
T18571 4187-4190 CC denotes and
T18572 4191-4198 VBN denotes covered
T18573 4199-4203 IN denotes with
T18574 4204-4211 RB denotes freshly
T18575 4212-4220 VBN denotes prepared
T18576 4221-4229 NN denotes collagen
T18577 4229-4230 . denotes .
T18578 4230-4292 sentence denotes Sepharose beads with an average diameter of 150 μm were used.
T18579 4231-4240 NN denotes Sepharose
T18580 4241-4246 NNS denotes beads
T18582 4247-4251 IN denotes with
T18583 4252-4254 DT denotes an
T18585 4255-4262 JJ denotes average
T18584 4263-4271 NN denotes diameter
T18586 4272-4274 IN denotes of
T18587 4275-4278 CD denotes 150
T18588 4279-4281 NN denotes μm
T18589 4282-4286 VBD denotes were
T18581 4287-4291 VBN denotes used
T18590 4291-4292 . denotes .
T18591 4292-4459 sentence denotes Beads were washed twice with PBS, air dried, and loaded with 10, 20, 50, or 100 ng/μl NT-3 (Collaborative Research, Chemicon) at 4 °C overnight with constant shaking.
T18592 4293-4298 NNS denotes Beads
T18594 4299-4303 VBD denotes were
T18593 4304-4310 VBN denotes washed
T18595 4311-4316 RB denotes twice
T18596 4317-4321 IN denotes with
T18597 4322-4325 NN denotes PBS
T18598 4325-4327 , denotes ,
T18599 4327-4330 NN denotes air
T18600 4331-4336 VBN denotes dried
T18601 4336-4338 , denotes ,
T18602 4338-4341 CC denotes and
T18603 4342-4348 VBN denotes loaded
T18604 4349-4353 IN denotes with
T18605 4354-4356 CD denotes 10
T18607 4356-4358 , denotes ,
T18608 4358-4360 CD denotes 20
T18609 4360-4362 , denotes ,
T18610 4362-4364 CD denotes 50
T18611 4364-4366 , denotes ,
T18612 4366-4368 CC denotes or
T18613 4369-4372 CD denotes 100
T18606 4373-4375 NN denotes ng
T18615 4375-4376 SYM denotes /
T18616 4376-4378 NN denotes μl
T18614 4379-4381 NN denotes NT
T18617 4381-4382 HYPH denotes -
T18618 4382-4383 CD denotes 3
T18619 4384-4385 -LRB- denotes (
T18621 4385-4398 JJ denotes Collaborative
T18620 4399-4407 NN denotes Research
T18622 4407-4409 , denotes ,
T18623 4409-4417 NNP denotes Chemicon
T18624 4417-4418 -RRB- denotes )
T18625 4419-4421 IN denotes at
T18626 4422-4423 CD denotes 4
T18627 4424-4426 NN denotes °C
T18628 4427-4436 RB denotes overnight
T18629 4437-4441 IN denotes with
T18630 4442-4450 JJ denotes constant
T18631 4451-4458 NN denotes shaking
T18632 4458-4459 . denotes .
T18633 4459-4531 sentence denotes For negative control, beads were loaded with BSA (10–100 ng/μl) or PBS.
T18634 4460-4463 IN denotes For
T18636 4464-4472 JJ denotes negative
T18637 4473-4480 NN denotes control
T18638 4480-4482 , denotes ,
T18639 4482-4487 NNS denotes beads
T18640 4488-4492 VBD denotes were
T18635 4493-4499 VBN denotes loaded
T18641 4500-4504 IN denotes with
T18642 4505-4508 NN denotes BSA
T18643 4509-4510 -LRB- denotes (
T18645 4510-4512 CD denotes 10
T18647 4512-4513 HYPH denotes –
T18646 4513-4516 CD denotes 100
T18644 4517-4519 NN denotes ng
T18648 4519-4520 SYM denotes /
T18649 4520-4522 NN denotes μl
T18650 4522-4523 -RRB- denotes )
T18651 4524-4526 CC denotes or
T18652 4527-4530 NN denotes PBS
T18653 4530-4531 . denotes .
T18654 4531-4674 sentence denotes Either a single neurotrophin-loaded bead or a single BSA (or PBS)–loaded bead was implanted about 200–1,200 μm away from the ganglion explant.
T18655 4532-4538 CC denotes Either
T18657 4539-4540 DT denotes a
T18658 4541-4547 JJ denotes single
T18659 4548-4560 NN denotes neurotrophin
T18661 4560-4561 HYPH denotes -
T18660 4561-4567 VBN denotes loaded
T18656 4568-4572 NN denotes bead
T18663 4573-4575 CC denotes or
T18664 4576-4577 DT denotes a
T18666 4578-4584 JJ denotes single
T18667 4585-4588 NN denotes BSA
T18669 4589-4590 -LRB- denotes (
T18670 4590-4592 CC denotes or
T18671 4593-4596 NN denotes PBS
T18672 4596-4597 -RRB- denotes )
T18673 4597-4598 HYPH denotes –
T18668 4598-4604 VBN denotes loaded
T18665 4605-4609 NN denotes bead
T18674 4610-4613 VBD denotes was
T18662 4614-4623 VBN denotes implanted
T18675 4624-4629 IN denotes about
T18677 4630-4633 CD denotes 200
T18678 4633-4634 HYPH denotes –
T18676 4634-4639 CD denotes 1,200
T18679 4640-4642 NN denotes μm
T18680 4643-4647 RB denotes away
T18681 4648-4652 IN denotes from
T18682 4653-4656 DT denotes the
T18684 4657-4665 NN denotes ganglion
T18683 4666-4673 NN denotes explant
T18685 4673-4674 . denotes .
T18686 4674-4764 sentence denotes Collagen-embedded cultures were then placed at 33 °C for 15 min for the matrix to harden.
T18687 4675-4683 NN denotes Collagen
T18689 4683-4684 HYPH denotes -
T18688 4684-4692 VBN denotes embedded
T18690 4693-4701 NNS denotes cultures
T18692 4702-4706 VBD denotes were
T18693 4707-4711 RB denotes then
T18691 4712-4718 VBN denotes placed
T18694 4719-4721 IN denotes at
T18695 4722-4724 CD denotes 33
T18696 4725-4727 NN denotes °C
T18697 4728-4731 IN denotes for
T18698 4732-4734 CD denotes 15
T18699 4735-4738 NN denotes min
T18700 4739-4742 IN denotes for
T18702 4743-4746 DT denotes the
T18703 4747-4753 NN denotes matrix
T18704 4754-4756 TO denotes to
T18701 4757-4763 VB denotes harden
T18705 4763-4764 . denotes .
T18706 4764-4819 sentence denotes Serum-free culture medium was then added to each well.
T18707 4765-4770 NN denotes Serum
T18709 4770-4771 HYPH denotes -
T18708 4771-4775 JJ denotes free
T18711 4776-4783 NN denotes culture
T18710 4784-4790 NN denotes medium
T18713 4791-4794 VBD denotes was
T18714 4795-4799 RB denotes then
T18712 4800-4805 VBN denotes added
T18715 4806-4808 IN denotes to
T18716 4809-4813 DT denotes each
T18717 4814-4818 NN denotes well
T18718 4818-4819 . denotes .
T18719 4819-4919 sentence denotes In cultures with WT DRG and control beads, 10% serum was added to ensure viability of the explants.
T18720 4820-4822 IN denotes In
T18722 4823-4831 NNS denotes cultures
T18723 4832-4836 IN denotes with
T18724 4837-4839 NN denotes WT
T18725 4840-4843 NN denotes DRG
T18726 4844-4847 CC denotes and
T18727 4848-4855 NN denotes control
T18728 4856-4861 NNS denotes beads
T18729 4861-4863 , denotes ,
T18730 4863-4865 CD denotes 10
T18731 4865-4866 NN denotes %
T18732 4867-4872 NN denotes serum
T18733 4873-4876 VBD denotes was
T18721 4877-4882 VBN denotes added
T18734 4883-4885 TO denotes to
T18735 4886-4892 VB denotes ensure
T18736 4893-4902 NN denotes viability
T18737 4903-4905 IN denotes of
T18738 4906-4909 DT denotes the
T18739 4910-4918 NNS denotes explants
T18740 4918-4919 . denotes .
T18741 4919-5037 sentence denotes TrkC-Fc (Regeneron Pharmaceuticals, Tarrytown, New York, United States) was added (20 μg/ml) into the culture medium.
T18742 4920-4924 NN denotes TrkC
T18744 4924-4925 HYPH denotes -
T18743 4925-4927 NN denotes Fc
T18746 4928-4929 -LRB- denotes (
T18748 4929-4938 NNP denotes Regeneron
T18747 4939-4954 NNP denotes Pharmaceuticals
T18749 4954-4956 , denotes ,
T18750 4956-4965 NNP denotes Tarrytown
T18751 4965-4967 , denotes ,
T18752 4967-4970 NNP denotes New
T18753 4971-4975 NNP denotes York
T18754 4975-4977 , denotes ,
T18755 4977-4983 NNP denotes United
T18756 4984-4990 NNP denotes States
T18757 4990-4991 -RRB- denotes )
T18758 4992-4995 VBD denotes was
T18745 4996-5001 VBN denotes added
T18759 5002-5003 -LRB- denotes (
T18761 5003-5005 CD denotes 20
T18760 5006-5008 NN denotes μg
T18762 5008-5009 SYM denotes /
T18763 5009-5011 NN denotes ml
T18764 5011-5012 -RRB- denotes )
T18765 5013-5017 IN denotes into
T18766 5018-5021 DT denotes the
T18768 5022-5029 NN denotes culture
T18767 5030-5036 NN denotes medium
T18769 5036-5037 . denotes .
T19117 5039-5046 NN denotes Explant
T19118 5047-5058 NNS denotes co-cultures
T19119 5058-5190 sentence denotes Spinal cords with attached DRGs were dissected out from Swiss Webster mouse embryos at E13, and sectioned into 300-μm-thick slices.
T19120 5059-5065 JJ denotes Spinal
T19121 5066-5071 NNS denotes cords
T19123 5072-5076 IN denotes with
T19124 5077-5085 VBN denotes attached
T19125 5086-5090 NNS denotes DRGs
T19126 5091-5095 VBD denotes were
T19122 5096-5105 VBN denotes dissected
T19127 5106-5109 RP denotes out
T19128 5110-5114 IN denotes from
T19129 5115-5120 NNP denotes Swiss
T19130 5121-5128 NNP denotes Webster
T19132 5129-5134 NN denotes mouse
T19131 5135-5142 NNS denotes embryos
T19133 5143-5145 IN denotes at
T19134 5146-5149 NN denotes E13
T19135 5149-5151 , denotes ,
T19136 5151-5154 CC denotes and
T19137 5155-5164 VBN denotes sectioned
T19138 5165-5169 IN denotes into
T19139 5170-5173 CD denotes 300
T19141 5173-5174 HYPH denotes -
T19140 5174-5176 NN denotes μm
T19143 5176-5177 HYPH denotes -
T19142 5177-5182 JJ denotes thick
T19144 5183-5189 NNS denotes slices
T19145 5189-5190 . denotes .
T19146 5190-5305 sentence denotes Tissue slices were placed on Millicell Tissue Culture Inserts (Millipore, Billrica, Massachusetts, United States).
T19147 5191-5197 NN denotes Tissue
T19148 5198-5204 NNS denotes slices
T19150 5205-5209 VBD denotes were
T19149 5210-5216 VBN denotes placed
T19151 5217-5219 IN denotes on
T19152 5220-5229 NN denotes Millicell
T19154 5230-5236 NN denotes Tissue
T19155 5237-5244 NN denotes Culture
T19153 5245-5252 NNS denotes Inserts
T19156 5253-5254 -LRB- denotes (
T19157 5254-5263 NNP denotes Millipore
T19158 5263-5265 , denotes ,
T19159 5265-5273 NNP denotes Billrica
T19160 5273-5275 , denotes ,
T19161 5275-5288 NNP denotes Massachusetts
T19162 5288-5290 , denotes ,
T19163 5290-5296 NNP denotes United
T19164 5297-5303 NNP denotes States
T19165 5303-5304 -RRB- denotes )
T19166 5304-5305 . denotes .
T19167 5305-5450 sentence denotes NT-3-loaded (n = 15) or PBS-loaded (n = 6) sepharose beads were prepared as described above, and placed in the midline at mid-spinal cord level.
T19168 5306-5308 NN denotes NT
T19170 5308-5309 HYPH denotes -
T19171 5309-5310 CD denotes 3
T19172 5310-5311 HYPH denotes -
T19169 5311-5317 VBN denotes loaded
T19174 5318-5319 -LRB- denotes (
T19176 5319-5320 NN denotes n
T19177 5321-5322 SYM denotes =
T19175 5323-5325 CD denotes 15
T19178 5325-5326 -RRB- denotes )
T19179 5327-5329 CC denotes or
T19180 5330-5333 NN denotes PBS
T19182 5333-5334 HYPH denotes -
T19181 5334-5340 VBN denotes loaded
T19183 5341-5342 -LRB- denotes (
T19185 5342-5343 NN denotes n
T19186 5344-5345 SYM denotes =
T19184 5346-5347 CD denotes 6
T19187 5347-5348 -RRB- denotes )
T19188 5349-5358 NN denotes sepharose
T19173 5359-5364 NNS denotes beads
T19190 5365-5369 VBD denotes were
T19189 5370-5378 VBN denotes prepared
T19191 5379-5381 IN denotes as
T19192 5382-5391 VBN denotes described
T19193 5392-5397 RB denotes above
T19194 5397-5399 , denotes ,
T19195 5399-5402 CC denotes and
T19196 5403-5409 VBN denotes placed
T19197 5410-5412 IN denotes in
T19198 5413-5416 DT denotes the
T19199 5417-5424 NN denotes midline
T19200 5425-5427 IN denotes at
T19201 5428-5438 JJ denotes mid-spinal
T19202 5439-5443 NN denotes cord
T19203 5444-5449 NN denotes level
T19204 5449-5450 . denotes .
T19205 5450-5593 sentence denotes Inserts were placed in six-well plates with serum-free medium at the bottom of the wells, and kept at 33 °C for 3 d in the presence of 5% CO2.
T19206 5451-5458 NNS denotes Inserts
T19208 5459-5463 VBD denotes were
T19207 5464-5470 VBN denotes placed
T19209 5471-5473 IN denotes in
T19210 5474-5477 CD denotes six
T19212 5477-5478 HYPH denotes -
T19211 5478-5482 NN denotes well
T19213 5483-5489 NNS denotes plates
T19214 5490-5494 IN denotes with
T19215 5495-5500 NN denotes serum
T19217 5500-5501 HYPH denotes -
T19216 5501-5505 JJ denotes free
T19218 5506-5512 NN denotes medium
T19219 5513-5515 IN denotes at
T19220 5516-5519 DT denotes the
T19221 5520-5526 NN denotes bottom
T19222 5527-5529 IN denotes of
T19223 5530-5533 DT denotes the
T19224 5534-5539 NNS denotes wells
T19225 5539-5541 , denotes ,
T19226 5541-5544 CC denotes and
T19227 5545-5549 VBD denotes kept
T19228 5550-5552 IN denotes at
T19229 5553-5555 CD denotes 33
T19230 5556-5558 NN denotes °C
T19231 5559-5562 IN denotes for
T19232 5563-5564 CD denotes 3
T19233 5565-5566 NN denotes d
T19234 5567-5569 IN denotes in
T19235 5570-5573 DT denotes the
T19236 5574-5582 NN denotes presence
T19237 5583-5585 IN denotes of
T19238 5586-5587 CD denotes 5
T19239 5587-5588 NN denotes %
T19240 5589-5592 NN denotes CO2
T19241 5592-5593 . denotes .
T19242 5593-5704 sentence denotes Cultures were then fixed with 4% paraformaldehyde in PBS, and small crystals of DiI were placed into the DRGs.
T19243 5594-5602 NNS denotes Cultures
T19245 5603-5607 VBD denotes were
T19246 5608-5612 RB denotes then
T19244 5613-5618 VBN denotes fixed
T19247 5619-5623 IN denotes with
T19248 5624-5625 CD denotes 4
T19249 5625-5626 NN denotes %
T19250 5627-5643 NN denotes paraformaldehyde
T19251 5644-5646 IN denotes in
T19252 5647-5650 NN denotes PBS
T19253 5650-5652 , denotes ,
T19254 5652-5655 CC denotes and
T19255 5656-5661 JJ denotes small
T19256 5662-5670 NNS denotes crystals
T19258 5671-5673 IN denotes of
T19259 5674-5677 NN denotes DiI
T19260 5678-5682 VBD denotes were
T19257 5683-5689 VBN denotes placed
T19261 5690-5694 IN denotes into
T19262 5695-5698 DT denotes the
T19263 5699-5703 NNS denotes DRGs
T19264 5703-5704 . denotes .
T19265 5704-5860 sentence denotes Samples were incubated in a 37-°C incubator, allowing the dye to diffuse, and photographed under a Nikon (Tokyo, Japan) inverted epifluoresence microscope.
T19266 5705-5712 NNS denotes Samples
T19268 5713-5717 VBD denotes were
T19267 5718-5727 VBN denotes incubated
T19269 5728-5730 IN denotes in
T19270 5731-5732 DT denotes a
T19272 5733-5735 CD denotes 37
T19274 5735-5736 HYPH denotes -
T19273 5736-5738 NN denotes °C
T19271 5739-5748 NN denotes incubator
T19275 5748-5750 , denotes ,
T19276 5750-5758 VBG denotes allowing
T19277 5759-5762 DT denotes the
T19278 5763-5766 NN denotes dye
T19280 5767-5769 TO denotes to
T19279 5770-5777 VB denotes diffuse
T19281 5777-5779 , denotes ,
T19282 5779-5782 CC denotes and
T19283 5783-5795 VBN denotes photographed
T19284 5796-5801 IN denotes under
T19285 5802-5803 DT denotes a
T19287 5804-5809 NNP denotes Nikon
T19288 5810-5811 -LRB- denotes (
T19289 5811-5816 NNP denotes Tokyo
T19290 5816-5818 , denotes ,
T19291 5818-5823 NNP denotes Japan
T19292 5823-5824 -RRB- denotes )
T19293 5825-5833 JJ denotes inverted
T19294 5834-5848 NN denotes epifluoresence
T19286 5849-5859 NN denotes microscope
T19295 5859-5860 . denotes .
R4627 T16204 T16203 prep of,Generation
R4628 T16205 T16206 amod double,KOs
R4629 T16206 T16204 pobj KOs,of
R4630 T16207 T16203 punct .,Generation
R4631 T16209 T16210 nsubj We,crossed
R4632 T16211 T16212 compound Bax,KO
R4633 T16212 T16213 compound KO,females
R4634 T16213 T16210 dobj females,crossed
R4635 T16214 T16213 prep on,females
R4636 T16215 T16216 det a,background
R4637 T16216 T16214 pobj background,on
R4638 T16217 T16216 nmod C57BL,background
R4639 T16218 T16217 punct /,C57BL
R4640 T16219 T16217 nummod 6,C57BL
R4641 T16220 T16221 punct (,Laboratory
R4642 T16221 T16213 parataxis Laboratory,females
R4643 T16222 T16221 compound Jackson,Laboratory
R4644 T16223 T16221 punct ", ",Laboratory
R4645 T16224 T16225 compound Bar,Harbor
R4646 T16225 T16221 npadvmod Harbor,Laboratory
R4647 T16226 T16221 punct ", ",Laboratory
R4648 T16227 T16221 npadvmod Maine,Laboratory
R4649 T16228 T16221 punct ", ",Laboratory
R4650 T16229 T16230 compound United,States
R4651 T16230 T16221 npadvmod States,Laboratory
R4652 T16231 T16221 punct ),Laboratory
R4653 T16232 T16210 prep to,crossed
R4654 T16233 T16234 nmod NT,heterozygote
R4655 T16234 T16237 compound heterozygote,males
R4656 T16235 T16233 punct -,NT
R4657 T16236 T16233 nummod 3,NT
R4658 T16237 T16232 pobj males,to
R4659 T16238 T16210 prep on,crossed
R4660 T16239 T16240 det a,background
R4661 T16240 T16238 pobj background,on
R4662 T16241 T16242 nummod 129,Sv
R4663 T16242 T16240 compound Sv,background
R4664 T16243 T16244 aux to,generate
R4665 T16244 T16210 advcl generate,crossed
R4666 T16245 T16246 amod double,heterozygote
R4667 T16246 T16247 compound heterozygote,animals
R4668 T16247 T16244 dobj animals,generate
R4669 T16248 T16210 punct .,crossed
R4670 T16250 T16251 nsubjpass Progeny,genotyped
R4671 T16252 T16251 auxpass was,genotyped
R4672 T16253 T16251 prep with,genotyped
R4673 T16254 T16253 pobj PCR,with
R4674 T16255 T16251 punct ", ",genotyped
R4675 T16256 T16251 cc and,genotyped
R4676 T16257 T16258 nsubjpass animals,bred
R4677 T16258 T16251 conj bred,genotyped
R4678 T16259 T16257 amod heterozygous,animals
R4679 T16260 T16259 prep for,heterozygous
R4680 T16261 T16262 det both,genes
R4681 T16262 T16260 pobj genes,for
R4682 T16263 T16258 auxpass were,bred
R4683 T16264 T16265 aux to,obtain
R4684 T16265 T16258 advcl obtain,bred
R4685 T16266 T16267 det the,KOs
R4686 T16267 T16265 dobj KOs,obtain
R4687 T16268 T16267 amod double,KOs
R4688 T16269 T16258 punct .,bred
R4689 T16271 T16272 nsubj Primers,were
R4690 T16273 T16271 acl used,Primers
R4691 T16274 T16273 prep for,used
R4692 T16275 T16276 det the,locus
R4693 T16276 T16274 pobj locus,for
R4694 T16277 T16276 compound Bax,locus
R4695 T16278 T16272 attr R661,were
R4696 T16279 T16278 punct ", ",R661
R4697 T16280 T16281 compound GTT,GG
R4698 T16281 T16278 appos GG,R661
R4699 T16282 T16281 compound GAC,GG
R4700 T16283 T16281 compound CAG,GG
R4701 T16284 T16281 compound AGT,GG
R4702 T16285 T16281 compound GGC,GG
R4703 T16286 T16281 compound GTA,GG
R4704 T16287 T16278 punct ;,R661
R4705 T16288 T16278 conj R662,R661
R4706 T16289 T16288 punct ", ",R662
R4707 T16290 T16291 compound CCG,GG
R4708 T16291 T16288 appos GG,R662
R4709 T16292 T16291 compound CTT,GG
R4710 T16293 T16291 compound CCA,GG
R4711 T16294 T16291 compound TTG,GG
R4712 T16295 T16291 compound CTC,GG
R4713 T16296 T16291 compound AGC,GG
R4714 T16297 T16288 punct ;,R662
R4715 T16298 T16288 cc and,R662
R4716 T16299 T16288 conj R663,R662
R4717 T16300 T16299 punct ", ",R663
R4718 T16301 T16302 compound GAG,ATG
R4719 T16302 T16299 appos ATG,R663
R4720 T16303 T16302 compound CTG,ATG
R4721 T16304 T16302 compound ATC,ATG
R4722 T16305 T16302 compound AGA,ATG
R4723 T16306 T16302 compound ACC,ATG
R4724 T16307 T16302 compound ATC,ATG
R4725 T16308 T16272 punct .,were
R4726 T16310 T16311 nsubj Primers,were
R4727 T16312 T16310 acl used,Primers
R4728 T16313 T16312 prep for,used
R4729 T16314 T16315 det the,locus
R4730 T16315 T16313 pobj locus,for
R4731 T16316 T16315 nmod NT,locus
R4732 T16317 T16316 punct -,NT
R4733 T16318 T16316 nummod 3,NT
R4734 T16319 T16311 attr NT3A,were
R4735 T16320 T16319 punct ", ",NT3A
R4736 T16321 T16322 compound CGT,AC
R4737 T16322 T16319 appos AC,NT3A
R4738 T16323 T16322 compound GGT,AC
R4739 T16324 T16322 compound GAG,AC
R4740 T16325 T16322 compound GTT,AC
R4741 T16326 T16322 compound CTA,AC
R4742 T16327 T16322 compound TTG,AC
R4743 T16328 T16322 compound GCT,AC
R4744 T16329 T16319 punct ;,NT3A
R4745 T16330 T16319 conj NT3B,NT3A
R4746 T16331 T16330 punct ", ",NT3B
R4747 T16332 T16333 compound CAG,GAG
R4748 T16333 T16330 appos GAG,NT3B
R4749 T16334 T16333 compound AGC,GAG
R4750 T16335 T16333 compound ACC,GAG
R4751 T16336 T16333 compound CTG,GAG
R4752 T16337 T16333 compound CCC,GAG
R4753 T16338 T16333 compound AAA,GAG
R4754 T16339 T16333 compound GCA,GAG
R4755 T16340 T16330 punct ;,NT3B
R4756 T16341 T16330 conj NT3R,NT3B
R4757 T16342 T16341 punct ", ",NT3R
R4758 T16343 T16344 compound CCT,CC
R4759 T16344 T16341 appos CC,NT3R
R4760 T16345 T16344 compound TGA,CC
R4761 T16346 T16344 compound CAA,CC
R4762 T16347 T16344 compound TAC,CC
R4763 T16348 T16344 compound TGA,CC
R4764 T16349 T16344 compound ATG,CC
R4765 T16350 T16341 punct ;,NT3R
R4766 T16351 T16341 cc and,NT3R
R4767 T16352 T16341 conj NEOF,NT3R
R4768 T16353 T16352 punct ", ",NEOF
R4769 T16354 T16355 compound GGG,AGG
R4770 T16355 T16352 appos AGG,NEOF
R4771 T16356 T16355 compound AAC,AGG
R4772 T16357 T16355 compound TTC,AGG
R4773 T16358 T16355 compound CTG,AGG
R4774 T16359 T16355 compound ACT,AGG
R4775 T16360 T16311 punct .,were
R4776 T16362 T16363 nmod WT,littermates
R4777 T16363 T16373 nsubjpass littermates,used
R4778 T16364 T16362 punct ", ",WT
R4779 T16365 T16366 compound Bax,KO
R4780 T16366 T16362 conj KO,WT
R4781 T16367 T16366 punct ", ",KO
R4782 T16368 T16366 cc and,KO
R4783 T16369 T16370 nmod NT,KO
R4784 T16370 T16366 conj KO,KO
R4785 T16371 T16369 punct -,NT
R4786 T16372 T16369 nummod 3,NT
R4787 T16374 T16373 auxpass were,used
R4788 T16375 T16373 prep as,used
R4789 T16376 T16375 pobj controls,as
R4790 T16377 T16373 punct .,used
R4791 T16379 T16380 det A,total
R4792 T16380 T16381 nsubjpass total,analyzed
R4793 T16381 T16393 ccomp analyzed,were
R4794 T16382 T16380 prep of,total
R4795 T16383 T16384 nummod 11,mice
R4796 T16384 T16382 pobj mice,of
R4797 T16385 T16386 nmod Bax,KO
R4798 T16386 T16384 compound KO,mice
R4799 T16387 T16385 punct /,Bax
R4800 T16388 T16385 appos NT,Bax
R4801 T16389 T16388 punct -,NT
R4802 T16390 T16388 nummod 3,NT
R4803 T16391 T16386 amod double,KO
R4804 T16392 T16381 auxpass were,analyzed
R4805 T16394 T16393 punct ", ",were
R4806 T16395 T16393 prep of,were
R4807 T16396 T16395 pobj these,of
R4808 T16397 T16393 nsubj nine,were
R4809 T16398 T16399 compound P0,pups
R4810 T16399 T16393 attr pups,were
R4811 T16400 T16393 punct .,were
R4812 T16402 T16403 det The,colony
R4813 T16403 T16405 nsubjpass colony,received
R4814 T16404 T16403 compound p75,colony
R4815 T16406 T16403 prep on,colony
R4816 T16407 T16408 det a,background
R4817 T16408 T16406 pobj background,on
R4818 T16409 T16410 nummod 129,S1
R4819 T16410 T16408 compound S1,background
R4820 T16411 T16405 auxpass was,received
R4821 T16412 T16405 prep from,received
R4822 T16413 T16414 compound Jackson,Laboratory
R4823 T16414 T16412 pobj Laboratory,from
R4824 T16415 T16405 punct .,received
R4825 T16417 T16418 nsubj We,used
R4826 T16419 T16420 compound tail,DNA
R4827 T16420 T16418 dobj DNA,used
R4828 T16421 T16422 aux to,genotype
R4829 T16422 T16418 advcl genotype,used
R4830 T16423 T16422 dobj animals,genotype
R4831 T16424 T16422 advcl using,genotype
R4832 T16425 T16426 det the,primers
R4833 T16426 T16424 dobj primers,using
R4834 T16427 T16426 appos IMR0013,primers
R4835 T16428 T16427 punct ", ",IMR0013
R4836 T16429 T16430 compound CTT,TC
R4837 T16430 T16427 appos TC,IMR0013
R4838 T16431 T16430 compound GGG,TC
R4839 T16432 T16430 compound TGG,TC
R4840 T16433 T16430 compound AGA,TC
R4841 T16434 T16430 compound GGC,TC
R4842 T16435 T16430 compound TAT,TC
R4843 T16436 T16427 punct ;,IMR0013
R4844 T16437 T16427 conj IMR0014,IMR0013
R4845 T16438 T16437 punct ", ",IMR0014
R4846 T16439 T16440 compound AGG,TC
R4847 T16440 T16437 appos TC,IMR0014
R4848 T16441 T16440 compound TGA,TC
R4849 T16442 T16440 compound GAT,TC
R4850 T16443 T16440 compound GAC,TC
R4851 T16444 T16440 compound AGG,TC
R4852 T16445 T16440 compound AGA,TC
R4853 T16446 T16437 punct (,IMR0014
R4854 T16447 T16448 amod generic,primers
R4855 T16448 T16437 appos primers,IMR0014
R4856 T16449 T16448 compound neo,primers
R4857 T16450 T16437 punct ),IMR0014
R4858 T16451 T16437 punct ;,IMR0014
R4859 T16452 T16437 conj IMR0710,IMR0014
R4860 T16453 T16452 punct ", ",IMR0710
R4861 T16454 T16455 compound TGT,GAG
R4862 T16455 T16452 appos GAG,IMR0710
R4863 T16456 T16455 compound TAC,GAG
R4864 T16457 T16455 compound GTT,GAG
R4865 T16458 T16455 compound CTC,GAG
R4866 T16459 T16455 compound TGA,GAG
R4867 T16460 T16455 compound CGT,GAG
R4868 T16461 T16455 compound GGT,GAG
R4869 T16462 T16452 punct ;,IMR0710
R4870 T16463 T16452 cc and,IMR0710
R4871 T16464 T16452 conj IMR0711,IMR0710
R4872 T16465 T16464 punct ", ",IMR0711
R4873 T16466 T16467 compound TCA,TC
R4874 T16467 T16464 appos TC,IMR0711
R4875 T16468 T16467 compound GCC,TC
R4876 T16469 T16467 compound CAG,TC
R4877 T16470 T16467 compound GGT,TC
R4878 T16471 T16467 compound GTG,TC
R4879 T16472 T16467 compound CAC,TC
R4880 T16473 T16474 punct (,locus
R4881 T16474 T16464 appos locus,IMR0711
R4882 T16475 T16474 compound p75,locus
R4883 T16476 T16464 punct ),IMR0711
R4884 T16477 T16418 punct .,used
R4885 T16479 T16480 prep For,considered
R4886 T16481 T16482 amod embryonic,experiments
R4887 T16482 T16479 pobj experiments,For
R4888 T16483 T16480 punct ", ",considered
R4889 T16484 T16480 nsubjpass day,considered
R4890 T16485 T16484 prep of,day
R4891 T16486 T16487 compound plug,positivity
R4892 T16487 T16485 pobj positivity,of
R4893 T16488 T16480 auxpass was,considered
R4894 T16489 T16480 oprd E0,considered
R4895 T16490 T16480 punct .,considered
R4896 T16492 T16493 nsubjpass All,approved
R4897 T16494 T16492 prep of,All
R4898 T16495 T16496 det the,protocols
R4899 T16496 T16494 pobj protocols,of
R4900 T16497 T16496 acl used,protocols
R4901 T16498 T16497 prep in,used
R4902 T16499 T16500 det this,study
R4903 T16500 T16498 pobj study,in
R4904 T16501 T16493 auxpass were,approved
R4905 T16502 T16493 agent by,approved
R4906 T16503 T16504 det the,Committee
R4907 T16504 T16502 pobj Committee,by
R4908 T16505 T16506 nmod Louisiana,University
R4909 T16506 T16508 nmod University,Center
R4910 T16507 T16506 nmod State,University
R4911 T16508 T16504 nmod Center,Committee
R4912 T16509 T16510 nmod Health,Sciences
R4913 T16510 T16508 nmod Sciences,Center
R4914 T16511 T16512 nmod Institutional,Animal
R4915 T16512 T16513 nmod Animal,Care
R4916 T16513 T16504 nmod Care,Committee
R4917 T16514 T16513 cc and,Care
R4918 T16515 T16513 conj Use,Care
R4919 T16516 T16493 cc and,approved
R4920 T16517 T16493 conj conformed,approved
R4921 T16518 T16517 prep to,conformed
R4922 T16519 T16520 det the,guidelines
R4923 T16520 T16518 pobj guidelines,to
R4924 T16521 T16522 nmod National,Institutes
R4925 T16522 T16520 nmod Institutes,guidelines
R4926 T16523 T16522 prep of,Institutes
R4927 T16524 T16523 pobj Health,of
R4928 T16525 T16520 prep for,guidelines
R4929 T16526 T16525 pobj use,for
R4930 T16527 T16526 prep of,use
R4931 T16528 T16529 amod experimental,animals
R4932 T16529 T16527 pobj animals,of
R4933 T16530 T16493 punct .,approved
R4934 T16880 T16881 compound TrkA,TrkC
R4935 T16881 T16883 compound TrkC,immunohistochemistry
R4936 T16882 T16881 punct /,TrkC
R4937 T16884 T16883 punct .,immunohistochemistry
R4938 T16886 T16887 amod Frozen,sections
R4939 T16887 T16890 nsubjpass sections,blocked
R4940 T16888 T16889 amod spinal,cord
R4941 T16889 T16887 compound cord,sections
R4942 T16891 T16887 punct (,sections
R4943 T16892 T16893 nummod 10,μm
R4944 T16893 T16894 npadvmod μm,thick
R4945 T16894 T16887 amod thick,sections
R4946 T16895 T16887 punct ),sections
R4947 T16896 T16890 auxpass were,blocked
R4948 T16897 T16890 cc and,blocked
R4949 T16898 T16890 conj incubated,blocked
R4950 T16899 T16898 prep in,incubated
R4951 T16900 T16901 det a,cocktail
R4952 T16901 T16899 pobj cocktail,in
R4953 T16902 T16901 prep of,cocktail
R4954 T16903 T16902 pobj rabbit,of
R4955 T16904 T16903 amod anti-TrkA,rabbit
R4956 T16905 T16903 cc and,rabbit
R4957 T16906 T16907 nmod goat,antibodies
R4958 T16907 T16903 conj antibodies,rabbit
R4959 T16908 T16906 amod anti-TrkC,goat
R4960 T16909 T16910 punct (,gift
R4961 T16910 T16907 parataxis gift,antibodies
R4962 T16911 T16910 prep of,gift
R4963 T16912 T16913 compound Dr.,Reichardt
R4964 T16913 T16911 pobj Reichardt,of
R4965 T16914 T16910 punct ;,gift
R4966 T16915 T16910 meta Huang,gift
R4967 T16916 T16915 nmod et,Huang
R4968 T16917 T16915 nmod al.,Huang
R4969 T16918 T16915 nummod 1999,Huang
R4970 T16919 T16910 punct ),gift
R4971 T16920 T16898 punct ", ",incubated
R4972 T16921 T16898 conj followed,incubated
R4973 T16922 T16921 agent by,followed
R4974 T16923 T16924 det a,cocktail
R4975 T16924 T16922 pobj cocktail,by
R4976 T16925 T16924 prep of,cocktail
R4977 T16926 T16927 npadvmod CY3,conjugated
R4978 T16927 T16928 amod conjugated,donkey
R4979 T16928 T16925 pobj donkey,of
R4980 T16929 T16928 amod anti-rabbit,donkey
R4981 T16930 T16928 cc and,donkey
R4982 T16931 T16932 npadvmod FITC,conjugated
R4983 T16932 T16933 amod conjugated,donkey
R4984 T16933 T16934 nmod donkey,antibodies
R4985 T16934 T16928 conj antibodies,donkey
R4986 T16935 T16933 amod anti-goat,donkey
R4987 T16936 T16937 punct (,Chemicon
R4988 T16937 T16924 parataxis Chemicon,cocktail
R4989 T16938 T16937 punct ", ",Chemicon
R4990 T16939 T16937 npadvmod Temecula,Chemicon
R4991 T16940 T16937 punct ", ",Chemicon
R4992 T16941 T16937 npadvmod California,Chemicon
R4993 T16942 T16937 punct ", ",Chemicon
R4994 T16943 T16944 compound United,States
R4995 T16944 T16937 npadvmod States,Chemicon
R4996 T16945 T16937 punct ),Chemicon
R4997 T16946 T16924 prep in,cocktail
R4998 T16947 T16948 det the,presence
R4999 T16948 T16946 pobj presence,in
R5000 T16949 T16948 prep of,presence
R5001 T16950 T16951 nummod 0.3,%
R5002 T16951 T16952 compound %,TritonX
R5003 T16952 T16949 pobj TritonX,of
R5004 T16953 T16952 punct -,TritonX
R5005 T16954 T16952 nummod 100,TritonX
R5006 T16955 T16952 cc and,TritonX
R5007 T16956 T16957 nummod 10,%
R5008 T16957 T16958 nmod %,serum
R5009 T16958 T16952 conj serum,TritonX
R5010 T16959 T16958 amod normal,serum
R5011 T16960 T16958 compound donkey,serum
R5012 T16961 T16890 punct .,blocked
R5013 T16963 T16964 prep For,reacted
R5014 T16965 T16966 compound PV,immunohistochemistry
R5015 T16966 T16963 pobj immunohistochemistry,For
R5016 T16967 T16964 punct ", ",reacted
R5017 T16968 T16964 nsubjpass sections,reacted
R5018 T16969 T16964 auxpass were,reacted
R5019 T16970 T16964 prep with,reacted
R5020 T16971 T16972 amod monoclonal,antibody
R5021 T16972 T16970 pobj antibody,with
R5022 T16973 T16972 nmod mouse,antibody
R5023 T16974 T16972 amod anti-PV,antibody
R5024 T16975 T16976 punct (,Sigma
R5025 T16976 T16972 parataxis Sigma,antibody
R5026 T16977 T16976 punct ", ",Sigma
R5027 T16978 T16979 compound St.,Louis
R5028 T16979 T16976 npadvmod Louis,Sigma
R5029 T16980 T16976 punct ", ",Sigma
R5030 T16981 T16976 npadvmod Missouri,Sigma
R5031 T16982 T16976 punct ", ",Sigma
R5032 T16983 T16984 compound United,States
R5033 T16984 T16976 npadvmod States,Sigma
R5034 T16985 T16976 punct ),Sigma
R5035 T16986 T16964 punct ", ",reacted
R5036 T16987 T16964 cc and,reacted
R5037 T16988 T16964 conj developed,reacted
R5038 T16989 T16988 agent by,developed
R5039 T16990 T16991 nmod Vector,fluorescein
R5040 T16991 T16992 nmod fluorescein,kit
R5041 T16992 T16989 pobj kit,by
R5042 T16993 T16992 nmod mouse,kit
R5043 T16994 T16993 prep on,mouse
R5044 T16995 T16994 pobj mouse,on
R5045 T16996 T16997 punct (,Laboratories
R5046 T16997 T16992 parataxis Laboratories,kit
R5047 T16998 T16997 compound Vector,Laboratories
R5048 T16999 T16997 punct ", ",Laboratories
R5049 T17000 T16997 npadvmod Burlingame,Laboratories
R5050 T17001 T16997 punct ", ",Laboratories
R5051 T17002 T16997 npadvmod California,Laboratories
R5052 T17003 T16997 punct ", ",Laboratories
R5053 T17004 T17005 compound United,States
R5054 T17005 T16997 npadvmod States,Laboratories
R5055 T17006 T16997 punct ),Laboratories
R5056 T17007 T16964 punct .,reacted
R5057 T17009 T17010 prep For,counted
R5058 T17011 T17012 compound quantification,purposes
R5059 T17012 T17009 pobj purposes,For
R5060 T17013 T17010 punct ", ",counted
R5061 T17014 T17015 npadvmod TrkA,labeled
R5062 T17015 T17020 amod labeled,cells
R5063 T17016 T17014 punct -,TrkA
R5064 T17017 T17014 cc and,TrkA
R5065 T17018 T17014 conj TrkC,TrkA
R5066 T17019 T17015 punct -,labeled
R5067 T17020 T17010 nsubjpass cells,counted
R5068 T17021 T17020 prep from,cells
R5069 T17022 T17023 nummod four,DRGs
R5070 T17023 T17021 pobj DRGs,from
R5071 T17024 T17023 amod different,DRGs
R5072 T17025 T17023 prep of,DRGs
R5073 T17026 T17027 det each,genotype
R5074 T17027 T17025 pobj genotype,of
R5075 T17028 T17027 cc and,genotype
R5076 T17029 T17027 conj age,genotype
R5077 T17030 T17027 acl studied,genotype
R5078 T17031 T17010 auxpass were,counted
R5079 T17032 T17010 punct ", ",counted
R5080 T17033 T17010 cc and,counted
R5081 T17034 T17035 nsubj ratios,plotted
R5082 T17035 T17010 conj plotted,counted
R5083 T17036 T17010 punct .,counted
R5084 T17352 T17353 compound Muscle,spindle
R5085 T17353 T17354 compound spindle,detection
R5086 T17355 T17354 punct .,detection
R5087 T17357 T17358 compound Gastrocnemius,muscle
R5088 T17358 T17359 nsubjpass muscle,dissected
R5089 T17360 T17359 auxpass was,dissected
R5090 T17361 T17359 prt out,dissected
R5091 T17362 T17359 prep in,dissected
R5092 T17363 T17364 compound P0,pups
R5093 T17364 T17362 pobj pups,in
R5094 T17365 T17359 cc and,dissected
R5095 T17366 T17359 conj sectioned,dissected
R5096 T17367 T17366 advmod longitudinally,sectioned
R5097 T17368 T17359 punct ", ",dissected
R5098 T17369 T17359 cc or,dissected
R5099 T17370 T17359 punct ", ",dissected
R5100 T17371 T17372 prep in,sectioned
R5101 T17372 T17359 conj sectioned,dissected
R5102 T17373 T17374 det some,cases
R5103 T17374 T17371 pobj cases,in
R5104 T17375 T17372 punct ", ",sectioned
R5105 T17376 T17377 det the,leg
R5106 T17377 T17372 nsubjpass leg,sectioned
R5107 T17378 T17377 amod whole,leg
R5108 T17379 T17377 prep at,leg
R5109 T17380 T17381 det the,level
R5110 T17381 T17379 pobj level,at
R5111 T17382 T17381 prep of,level
R5112 T17383 T17384 compound gastrocnemius,muscle
R5113 T17384 T17382 pobj muscle,of
R5114 T17385 T17384 prep with,muscle
R5115 T17386 T17387 amod tibial,nerve
R5116 T17387 T17385 pobj nerve,with
R5117 T17388 T17372 auxpass was,sectioned
R5118 T17389 T17372 prep in,sectioned
R5119 T17390 T17391 compound cross,section
R5120 T17391 T17389 pobj section,in
R5121 T17392 T17372 punct .,sectioned
R5122 T17394 T17395 nsubj Sections,incubated
R5123 T17396 T17395 aux were,incubated
R5124 T17397 T17395 prep with,incubated
R5125 T17398 T17399 amod monoclonal,antibody
R5126 T17399 T17397 pobj antibody,with
R5127 T17400 T17399 compound S46,antibody
R5128 T17401 T17402 punct (,gift
R5129 T17402 T17399 parataxis gift,antibody
R5130 T17403 T17402 prep of,gift
R5131 T17404 T17405 compound Dr.,Stockdale
R5132 T17405 T17403 pobj Stockdale,of
R5133 T17406 T17402 punct ),gift
R5134 T17407 T17399 amod reactive,antibody
R5135 T17408 T17407 prep to,reactive
R5136 T17409 T17410 det the,isoform
R5137 T17410 T17408 pobj isoform,to
R5138 T17411 T17412 npadvmod spindle,specific
R5139 T17412 T17410 amod specific,isoform
R5140 T17413 T17412 punct -,specific
R5141 T17414 T17415 amod slow,tonic
R5142 T17415 T17410 nmod tonic,isoform
R5143 T17416 T17415 punct -,tonic
R5144 T17417 T17410 nmod myosin,isoform
R5145 T17418 T17419 amod heavy,chain
R5146 T17419 T17410 compound chain,isoform
R5147 T17420 T17395 cc and,incubated
R5148 T17421 T17395 conj developed,incubated
R5149 T17422 T17421 prep by,developed
R5150 T17423 T17424 nmod Vector,kit
R5151 T17424 T17422 pobj kit,by
R5152 T17425 T17424 nmod mouse,kit
R5153 T17426 T17425 prep on,mouse
R5154 T17427 T17426 pobj mouse,on
R5155 T17428 T17429 mark as,described
R5156 T17429 T17421 advcl described,developed
R5157 T17430 T17429 advmod above,described
R5158 T17431 T17395 punct ", ",incubated
R5159 T17432 T17395 advcl followed,incubated
R5160 T17433 T17432 agent by,followed
R5161 T17434 T17435 nmod rabbit,antibody
R5162 T17435 T17433 pobj antibody,by
R5163 T17436 T17437 amod anti-NF,M
R5164 T17437 T17435 compound M,antibody
R5165 T17438 T17437 punct -,M
R5166 T17439 T17440 punct (,Chemicon
R5167 T17440 T17435 parataxis Chemicon,antibody
R5168 T17441 T17440 punct ),Chemicon
R5169 T17442 T17435 cc and,antibody
R5170 T17443 T17444 nmod CY3,antibody
R5171 T17444 T17435 conj antibody,antibody
R5172 T17445 T17444 amod conjugated,antibody
R5173 T17446 T17444 nmod goat,antibody
R5174 T17447 T17444 amod anti-rabbit,antibody
R5175 T17448 T17449 punct (,Chemicon
R5176 T17449 T17444 parataxis Chemicon,antibody
R5177 T17450 T17449 punct ),Chemicon
R5178 T17451 T17432 prep in,followed
R5179 T17452 T17453 det a,protocol
R5180 T17453 T17451 pobj protocol,in
R5181 T17454 T17453 amod sequential,protocol
R5182 T17455 T17456 amod double,labeling
R5183 T17456 T17453 compound labeling,protocol
R5184 T17457 T17456 punct -,labeling
R5185 T17458 T17395 punct .,incubated
R5186 T17460 T17461 prep In,placed
R5187 T17462 T17463 det another,method
R5188 T17463 T17460 pobj method,In
R5189 T17464 T17461 punct ", ",placed
R5190 T17465 T17466 compound DiI,crystals
R5191 T17466 T17461 nsubjpass crystals,placed
R5192 T17467 T17461 auxpass were,placed
R5193 T17468 T17461 prep in,placed
R5194 T17469 T17468 pobj DRGs,in
R5195 T17470 T17469 prep of,DRGs
R5196 T17471 T17472 compound E17,embryos
R5197 T17472 T17470 pobj embryos,of
R5198 T17473 T17472 prep from,embryos
R5199 T17474 T17475 amod different,genotypes
R5200 T17475 T17473 pobj genotypes,from
R5201 T17476 T17477 punct (,2
R5202 T17477 T17472 parataxis 2,embryos
R5203 T17478 T17477 nsubj n,2
R5204 T17479 T17477 punct =,2
R5205 T17480 T17477 punct ),2
R5206 T17481 T17461 punct ", ",placed
R5207 T17482 T17461 cc and,placed
R5208 T17483 T17484 det the,muscle
R5209 T17484 T17486 nsubjpass muscle,isolated
R5210 T17485 T17484 compound gastrocnemius,muscle
R5211 T17486 T17461 conj isolated,placed
R5212 T17487 T17486 auxpass was,isolated
R5213 T17488 T17486 cc and,isolated
R5214 T17489 T17486 conj sectioned,isolated
R5215 T17490 T17489 prep into,sectioned
R5216 T17491 T17492 nummod 40,μm
R5217 T17492 T17494 npadvmod μm,thick
R5218 T17493 T17492 punct -,μm
R5219 T17494 T17496 amod thick,slices
R5220 T17495 T17494 punct -,thick
R5221 T17496 T17490 pobj slices,into
R5222 T17497 T17489 prep on,sectioned
R5223 T17498 T17499 det a,vibratome
R5224 T17499 T17497 pobj vibratome,on
R5225 T17500 T17486 punct .,isolated
R5226 T17771 T17772 compound DiI,labeling
R5227 T17773 T17772 punct .,labeling
R5228 T17775 T17776 amod Spinal,cords
R5229 T17776 T17777 nsubjpass cords,dissected
R5230 T17778 T17777 auxpass were,dissected
R5231 T17779 T17777 prt out,dissected
R5232 T17780 T17781 mark with,attached
R5233 T17781 T17777 advcl attached,dissected
R5234 T17782 T17783 det the,DRGs
R5235 T17783 T17781 nsubj DRGs,attached
R5236 T17784 T17777 punct ", ",dissected
R5237 T17785 T17786 compound motor,root
R5238 T17786 T17787 nsubjpass root,cut
R5239 T17787 T17777 conj cut,dissected
R5240 T17788 T17787 auxpass was,cut
R5241 T17789 T17790 aux to,prevent
R5242 T17790 T17787 advcl prevent,cut
R5243 T17791 T17790 dobj backfilling,prevent
R5244 T17792 T17791 prep of,backfilling
R5245 T17793 T17794 compound motor,neurons
R5246 T17794 T17792 pobj neurons,of
R5247 T17795 T17787 punct ", ",cut
R5248 T17796 T17787 cc and,cut
R5249 T17797 T17798 nsubjpass crystals,inserted
R5250 T17798 T17787 conj inserted,cut
R5251 T17799 T17797 prep of,crystals
R5252 T17800 T17801 nummod 1,perchlorate
R5253 T17801 T17799 pobj perchlorate,of
R5254 T17802 T17800 punct ",",1
R5255 T17803 T17800 appos 1,1
R5256 T17804 T17803 punct ′,1
R5257 T17805 T17801 punct -,perchlorate
R5258 T17806 T17801 nmod dioctadecyl,perchlorate
R5259 T17807 T17801 punct -,perchlorate
R5260 T17808 T17801 nummod 3,perchlorate
R5261 T17809 T17808 punct ",",3
R5262 T17810 T17808 appos 3,3
R5263 T17811 T17808 punct ",",3
R5264 T17812 T17808 appos 3,3
R5265 T17813 T17812 punct ′,3
R5266 T17814 T17808 punct ",",3
R5267 T17815 T17808 appos 3,3
R5268 T17816 T17815 punct ′,3
R5269 T17817 T17801 punct -,perchlorate
R5270 T17818 T17801 compound tetramethylindocarbocyanine,perchlorate
R5271 T17819 T17820 punct (,Probes
R5272 T17820 T17797 parataxis Probes,crystals
R5273 T17821 T17820 dep DiI,Probes
R5274 T17822 T17820 punct ;,Probes
R5275 T17823 T17820 compound Molecular,Probes
R5276 T17824 T17820 punct ", ",Probes
R5277 T17825 T17820 npadvmod Eugene,Probes
R5278 T17826 T17820 punct ", ",Probes
R5279 T17827 T17820 npadvmod Oregon,Probes
R5280 T17828 T17820 punct ", ",Probes
R5281 T17829 T17830 compound United,States
R5282 T17830 T17820 npadvmod States,Probes
R5283 T17831 T17820 punct ),Probes
R5284 T17832 T17798 auxpass were,inserted
R5285 T17833 T17798 prep in,inserted
R5286 T17834 T17833 pobj DRGs,in
R5287 T17835 T17798 punct .,inserted
R5288 T17837 T17838 amod Labeled,cords
R5289 T17838 T17840 nsubjpass cords,incubated
R5290 T17839 T17838 amod spinal,cords
R5291 T17841 T17840 auxpass were,incubated
R5292 T17842 T17840 prep at,incubated
R5293 T17843 T17844 nummod 37,°C
R5294 T17844 T17842 pobj °C,at
R5295 T17845 T17840 prep for,incubated
R5296 T17846 T17847 nummod 8,d
R5297 T17847 T17845 pobj d,for
R5298 T17848 T17840 cc and,incubated
R5299 T17849 T17840 conj sectioned,incubated
R5300 T17850 T17849 prep on,sectioned
R5301 T17851 T17852 det a,vibrotome
R5302 T17852 T17850 pobj vibrotome,on
R5303 T17853 T17849 prep at,sectioned
R5304 T17854 T17855 nummod 100,μm
R5305 T17855 T17853 pobj μm,at
R5306 T17856 T17840 punct .,incubated
R5307 T17858 T17859 nsubjpass Sections,observed
R5308 T17860 T17859 auxpass were,observed
R5309 T17861 T17859 prep under,observed
R5310 T17862 T17863 det the,microscope
R5311 T17863 T17861 pobj microscope,under
R5312 T17864 T17863 amod fluorescent,microscope
R5313 T17865 T17859 cc and,observed
R5314 T17866 T17859 conj photographed,observed
R5315 T17867 T17859 punct ", ",observed
R5316 T17868 T17859 cc and,observed
R5317 T17869 T17870 nsubjpass images,transferred
R5318 T17870 T17859 conj transferred,observed
R5319 T17871 T17870 auxpass were,transferred
R5320 T17872 T17870 prep to,transferred
R5321 T17873 T17874 compound Adobe,Photoshop
R5322 T17874 T17872 pobj Photoshop,to
R5323 T17875 T17870 punct ", ",transferred
R5324 T17876 T17870 conj inverted,transferred
R5325 T17877 T17876 punct ", ",inverted
R5326 T17878 T17876 cc and,inverted
R5327 T17879 T17876 conj adjusted,inverted
R5328 T17880 T17879 prep for,adjusted
R5329 T17881 T17880 pobj brightness,for
R5330 T17882 T17881 cc and,brightness
R5331 T17883 T17881 conj contrast,brightness
R5332 T17884 T17870 punct .,transferred
R5333 T17887 T17886 prep in,Photoconverted
R5334 T17888 T17889 nummod 0.15,%
R5335 T17889 T17890 compound %,DAB
R5336 T17890 T17887 pobj DAB,in
R5337 T17891 T17892 punct (,Sigma
R5338 T17892 T17890 parataxis Sigma,DAB
R5339 T17893 T17894 nummod 3,diaminobenzidine
R5340 T17894 T17892 dep diaminobenzidine,Sigma
R5341 T17895 T17893 punct ",",3
R5342 T17896 T17893 appos 3,3
R5343 T17897 T17896 punct ′,3
R5344 T17898 T17894 punct -,diaminobenzidine
R5345 T17899 T17892 punct ;,Sigma
R5346 T17900 T17892 punct ),Sigma
R5347 T17901 T17886 prep in,Photoconverted
R5348 T17902 T17903 nummod 0.1,M
R5349 T17903 T17904 compound M,buffer
R5350 T17904 T17901 pobj buffer,in
R5351 T17905 T17904 compound Tris,buffer
R5352 T17906 T17904 punct (,buffer
R5353 T17907 T17904 npadvmod pH,buffer
R5354 T17908 T17907 nummod 8.2,pH
R5355 T17909 T17904 punct ),buffer
R5356 T17910 T17886 punct .,Photoconverted
R5357 T18468 T18469 advmod In,vitro
R5358 T18469 T18470 amod vitro,assay
R5359 T18471 T18470 compound co-culture,assay
R5360 T18472 T18470 punct .,assay
R5361 T18474 T18475 compound DRG,explants
R5362 T18475 T18476 nsubjpass explants,derived
R5363 T18477 T18476 auxpass were,derived
R5364 T18478 T18476 prep from,derived
R5365 T18479 T18480 nmod Swiss,Webster
R5366 T18480 T18481 nmod Webster,embryos
R5367 T18481 T18478 pobj embryos,from
R5368 T18482 T18480 punct ", ",Webster
R5369 T18483 T18484 npadvmod Bax,null
R5370 T18484 T18480 conj null,Webster
R5371 T18485 T18484 punct ", ",null
R5372 T18486 T18484 cc and,null
R5373 T18487 T18488 npadvmod p75,null
R5374 T18488 T18484 conj null,null
R5375 T18489 T18481 compound mouse,embryos
R5376 T18490 T18491 punct (,E13
R5377 T18491 T18481 parataxis E13,embryos
R5378 T18492 T18491 punct ),E13
R5379 T18493 T18476 punct .,derived
R5380 T18495 T18496 prep For,dissected
R5381 T18497 T18498 compound collagen,matrix
R5382 T18498 T18499 compound matrix,assays
R5383 T18499 T18495 pobj assays,For
R5384 T18500 T18496 punct ", ",dissected
R5385 T18501 T18496 nsubjpass DRGs,dissected
R5386 T18502 T18496 auxpass were,dissected
R5387 T18503 T18496 prt out,dissected
R5388 T18504 T18496 prep under,dissected
R5389 T18505 T18506 det a,stereomicroscope
R5390 T18506 T18504 pobj stereomicroscope,under
R5391 T18507 T18496 advcl using,dissected
R5392 T18508 T18509 compound tungsten,needles
R5393 T18509 T18507 dobj needles,using
R5394 T18510 T18496 punct .,dissected
R5395 T18512 T18513 compound Collagen,matrix
R5396 T18513 T18514 nsubjpass matrix,prepared
R5397 T18515 T18514 auxpass was,prepared
R5398 T18516 T18514 prep with,prepared
R5399 T18517 T18518 nummod 430,μl
R5400 T18518 T18516 pobj μl,with
R5401 T18519 T18518 prep of,μl
R5402 T18520 T18519 pobj collagen,of
R5403 T18521 T18522 punct (,Sigma
R5404 T18522 T18518 parataxis Sigma,μl
R5405 T18523 T18524 nummod 3,mg
R5406 T18524 T18522 dep mg,Sigma
R5407 T18525 T18526 punct /,ml
R5408 T18526 T18524 prep ml,mg
R5409 T18527 T18524 acl dissolved,mg
R5410 T18528 T18527 prep in,dissolved
R5411 T18529 T18530 nummod 0.1,M
R5412 T18530 T18531 nmod M,acid
R5413 T18531 T18528 pobj acid,in
R5414 T18532 T18531 amod acetic,acid
R5415 T18533 T18522 punct ", ",Sigma
R5416 T18534 T18522 punct ),Sigma
R5417 T18535 T18518 punct ", ",μl
R5418 T18536 T18537 nummod 50,μl
R5419 T18537 T18518 conj μl,μl
R5420 T18538 T18537 prep of,μl
R5421 T18539 T18540 nummod 10,DMEM
R5422 T18540 T18542 compound DMEM,medium
R5423 T18541 T18540 compound X,DMEM
R5424 T18542 T18538 pobj medium,of
R5425 T18543 T18537 punct ", ",μl
R5426 T18544 T18537 cc and,μl
R5427 T18545 T18546 nummod 2.5,μl
R5428 T18546 T18537 conj μl,μl
R5429 T18547 T18546 prep of,μl
R5430 T18548 T18549 nummod 0.8,M
R5431 T18549 T18550 compound M,NaCa2
R5432 T18550 T18547 pobj NaCa2,of
R5433 T18551 T18514 punct ", ",prepared
R5434 T18552 T18514 cc and,prepared
R5435 T18553 T18554 det the,pH
R5436 T18554 T18555 nsubjpass pH,adjusted
R5437 T18555 T18514 conj adjusted,prepared
R5438 T18556 T18555 auxpass was,adjusted
R5439 T18557 T18555 prep to,adjusted
R5440 T18558 T18557 pobj 7.5,to
R5441 T18559 T18555 punct .,adjusted
R5442 T18561 T18562 amod Individual,explants
R5443 T18562 T18564 nsubjpass explants,placed
R5444 T18563 T18562 compound ganglion,explants
R5445 T18565 T18564 auxpass were,placed
R5446 T18566 T18564 prep in,placed
R5447 T18567 T18568 nummod 24,well
R5448 T18568 T18570 compound well,plates
R5449 T18569 T18568 punct -,well
R5450 T18570 T18566 pobj plates,in
R5451 T18571 T18564 cc and,placed
R5452 T18572 T18564 conj covered,placed
R5453 T18573 T18572 prep with,covered
R5454 T18574 T18575 advmod freshly,prepared
R5455 T18575 T18576 amod prepared,collagen
R5456 T18576 T18573 pobj collagen,with
R5457 T18577 T18564 punct .,placed
R5458 T18579 T18580 compound Sepharose,beads
R5459 T18580 T18581 nsubjpass beads,used
R5460 T18582 T18580 prep with,beads
R5461 T18583 T18584 det an,diameter
R5462 T18584 T18582 pobj diameter,with
R5463 T18585 T18584 amod average,diameter
R5464 T18586 T18584 prep of,diameter
R5465 T18587 T18588 nummod 150,μm
R5466 T18588 T18586 pobj μm,of
R5467 T18589 T18581 auxpass were,used
R5468 T18590 T18581 punct .,used
R5469 T18592 T18593 nsubjpass Beads,washed
R5470 T18594 T18593 auxpass were,washed
R5471 T18595 T18593 advmod twice,washed
R5472 T18596 T18593 prep with,washed
R5473 T18597 T18596 pobj PBS,with
R5474 T18598 T18593 punct ", ",washed
R5475 T18599 T18600 dep air,dried
R5476 T18600 T18593 conj dried,washed
R5477 T18601 T18600 punct ", ",dried
R5478 T18602 T18600 cc and,dried
R5479 T18603 T18600 conj loaded,dried
R5480 T18604 T18603 prep with,loaded
R5481 T18605 T18606 nummod 10,ng
R5482 T18606 T18614 nmod ng,NT
R5483 T18607 T18605 punct ", ",10
R5484 T18608 T18605 conj 20,10
R5485 T18609 T18608 punct ", ",20
R5486 T18610 T18608 conj 50,20
R5487 T18611 T18610 punct ", ",50
R5488 T18612 T18610 cc or,50
R5489 T18613 T18610 conj 100,50
R5490 T18614 T18604 pobj NT,with
R5491 T18615 T18616 punct /,μl
R5492 T18616 T18606 prep μl,ng
R5493 T18617 T18614 punct -,NT
R5494 T18618 T18614 nummod 3,NT
R5495 T18619 T18620 punct (,Research
R5496 T18620 T18614 parataxis Research,NT
R5497 T18621 T18620 amod Collaborative,Research
R5498 T18622 T18620 punct ", ",Research
R5499 T18623 T18620 npadvmod Chemicon,Research
R5500 T18624 T18620 punct ),Research
R5501 T18625 T18603 prep at,loaded
R5502 T18626 T18627 nummod 4,°C
R5503 T18627 T18625 pobj °C,at
R5504 T18628 T18603 advmod overnight,loaded
R5505 T18629 T18603 prep with,loaded
R5506 T18630 T18631 amod constant,shaking
R5507 T18631 T18629 pobj shaking,with
R5508 T18632 T18593 punct .,washed
R5509 T18634 T18635 prep For,loaded
R5510 T18636 T18637 amod negative,control
R5511 T18637 T18634 pobj control,For
R5512 T18638 T18635 punct ", ",loaded
R5513 T18639 T18635 nsubjpass beads,loaded
R5514 T18640 T18635 auxpass were,loaded
R5515 T18641 T18635 prep with,loaded
R5516 T18642 T18641 pobj BSA,with
R5517 T18643 T18644 punct (,ng
R5518 T18644 T18642 parataxis ng,BSA
R5519 T18645 T18646 compound 10,100
R5520 T18646 T18644 nummod 100,ng
R5521 T18647 T18646 punct –,100
R5522 T18648 T18649 punct /,μl
R5523 T18649 T18644 prep μl,ng
R5524 T18650 T18644 punct ),ng
R5525 T18651 T18642 cc or,BSA
R5526 T18652 T18642 conj PBS,BSA
R5527 T18653 T18635 punct .,loaded
R5528 T18655 T18656 preconj Either,bead
R5529 T18656 T18662 nsubjpass bead,implanted
R5530 T18657 T18656 det a,bead
R5531 T18658 T18656 amod single,bead
R5532 T18659 T18660 npadvmod neurotrophin,loaded
R5533 T18660 T18656 amod loaded,bead
R5534 T18661 T18660 punct -,loaded
R5535 T18663 T18656 cc or,bead
R5536 T18664 T18665 det a,bead
R5537 T18665 T18656 conj bead,bead
R5538 T18666 T18665 amod single,bead
R5539 T18667 T18668 npadvmod BSA,loaded
R5540 T18668 T18665 amod loaded,bead
R5541 T18669 T18667 punct (,BSA
R5542 T18670 T18667 cc or,BSA
R5543 T18671 T18667 conj PBS,BSA
R5544 T18672 T18671 punct ),PBS
R5545 T18673 T18668 punct –,loaded
R5546 T18674 T18662 auxpass was,implanted
R5547 T18675 T18676 quantmod about,"1,200"
R5548 T18676 T18679 nummod "1,200",μm
R5549 T18677 T18676 compound 200,"1,200"
R5550 T18678 T18676 punct –,"1,200"
R5551 T18679 T18680 npadvmod μm,away
R5552 T18680 T18662 advmod away,implanted
R5553 T18681 T18680 prep from,away
R5554 T18682 T18683 det the,explant
R5555 T18683 T18681 pobj explant,from
R5556 T18684 T18683 compound ganglion,explant
R5557 T18685 T18662 punct .,implanted
R5558 T18687 T18688 npadvmod Collagen,embedded
R5559 T18688 T18690 amod embedded,cultures
R5560 T18689 T18688 punct -,embedded
R5561 T18690 T18691 nsubjpass cultures,placed
R5562 T18692 T18691 auxpass were,placed
R5563 T18693 T18691 advmod then,placed
R5564 T18694 T18691 prep at,placed
R5565 T18695 T18696 nummod 33,°C
R5566 T18696 T18694 pobj °C,at
R5567 T18697 T18691 prep for,placed
R5568 T18698 T18699 nummod 15,min
R5569 T18699 T18697 pobj min,for
R5570 T18700 T18701 mark for,harden
R5571 T18701 T18691 advcl harden,placed
R5572 T18702 T18703 det the,matrix
R5573 T18703 T18701 nsubj matrix,harden
R5574 T18704 T18701 aux to,harden
R5575 T18705 T18691 punct .,placed
R5576 T18707 T18708 npadvmod Serum,free
R5577 T18708 T18710 amod free,medium
R5578 T18709 T18708 punct -,free
R5579 T18710 T18712 nsubjpass medium,added
R5580 T18711 T18710 compound culture,medium
R5581 T18713 T18712 auxpass was,added
R5582 T18714 T18712 advmod then,added
R5583 T18715 T18712 prep to,added
R5584 T18716 T18717 det each,well
R5585 T18717 T18715 pobj well,to
R5586 T18718 T18712 punct .,added
R5587 T18720 T18721 prep In,added
R5588 T18722 T18720 pobj cultures,In
R5589 T18723 T18722 prep with,cultures
R5590 T18724 T18725 compound WT,DRG
R5591 T18725 T18723 pobj DRG,with
R5592 T18726 T18725 cc and,DRG
R5593 T18727 T18728 compound control,beads
R5594 T18728 T18725 conj beads,DRG
R5595 T18729 T18721 punct ", ",added
R5596 T18730 T18731 nummod 10,%
R5597 T18731 T18732 compound %,serum
R5598 T18732 T18721 nsubjpass serum,added
R5599 T18733 T18721 auxpass was,added
R5600 T18734 T18735 aux to,ensure
R5601 T18735 T18721 advcl ensure,added
R5602 T18736 T18735 dobj viability,ensure
R5603 T18737 T18736 prep of,viability
R5604 T18738 T18739 det the,explants
R5605 T18739 T18737 pobj explants,of
R5606 T18740 T18721 punct .,added
R5607 T18742 T18743 compound TrkC,Fc
R5608 T18743 T18745 nsubjpass Fc,added
R5609 T18744 T18743 punct -,Fc
R5610 T18746 T18747 punct (,Pharmaceuticals
R5611 T18747 T18743 parataxis Pharmaceuticals,Fc
R5612 T18748 T18747 compound Regeneron,Pharmaceuticals
R5613 T18749 T18747 punct ", ",Pharmaceuticals
R5614 T18750 T18747 npadvmod Tarrytown,Pharmaceuticals
R5615 T18751 T18747 punct ", ",Pharmaceuticals
R5616 T18752 T18753 compound New,York
R5617 T18753 T18747 npadvmod York,Pharmaceuticals
R5618 T18754 T18747 punct ", ",Pharmaceuticals
R5619 T18755 T18756 compound United,States
R5620 T18756 T18747 npadvmod States,Pharmaceuticals
R5621 T18757 T18747 punct ),Pharmaceuticals
R5622 T18758 T18745 auxpass was,added
R5623 T18759 T18760 punct (,μg
R5624 T18760 T18745 parataxis μg,added
R5625 T18761 T18760 nummod 20,μg
R5626 T18762 T18763 punct /,ml
R5627 T18763 T18760 prep ml,μg
R5628 T18764 T18760 punct ),μg
R5629 T18765 T18745 prep into,added
R5630 T18766 T18767 det the,medium
R5631 T18767 T18765 pobj medium,into
R5632 T18768 T18767 compound culture,medium
R5633 T18769 T18745 punct .,added
R5634 T19117 T19118 compound Explant,co-cultures
R5635 T19120 T19121 amod Spinal,cords
R5636 T19121 T19122 nsubjpass cords,dissected
R5637 T19123 T19121 prep with,cords
R5638 T19124 T19125 amod attached,DRGs
R5639 T19125 T19123 pobj DRGs,with
R5640 T19126 T19122 auxpass were,dissected
R5641 T19127 T19122 prt out,dissected
R5642 T19128 T19122 prep from,dissected
R5643 T19129 T19130 compound Swiss,Webster
R5644 T19130 T19131 compound Webster,embryos
R5645 T19131 T19128 pobj embryos,from
R5646 T19132 T19131 compound mouse,embryos
R5647 T19133 T19131 prep at,embryos
R5648 T19134 T19133 pobj E13,at
R5649 T19135 T19122 punct ", ",dissected
R5650 T19136 T19122 cc and,dissected
R5651 T19137 T19122 conj sectioned,dissected
R5652 T19138 T19137 prep into,sectioned
R5653 T19139 T19140 nummod 300,μm
R5654 T19140 T19142 npadvmod μm,thick
R5655 T19141 T19140 punct -,μm
R5656 T19142 T19144 amod thick,slices
R5657 T19143 T19142 punct -,thick
R5658 T19144 T19138 pobj slices,into
R5659 T19145 T19122 punct .,dissected
R5660 T19147 T19148 compound Tissue,slices
R5661 T19148 T19149 nsubjpass slices,placed
R5662 T19150 T19149 auxpass were,placed
R5663 T19151 T19149 prep on,placed
R5664 T19152 T19153 compound Millicell,Inserts
R5665 T19153 T19151 pobj Inserts,on
R5666 T19154 T19155 compound Tissue,Culture
R5667 T19155 T19153 compound Culture,Inserts
R5668 T19156 T19157 punct (,Millipore
R5669 T19157 T19153 parataxis Millipore,Inserts
R5670 T19158 T19157 punct ", ",Millipore
R5671 T19159 T19157 npadvmod Billrica,Millipore
R5672 T19160 T19157 punct ", ",Millipore
R5673 T19161 T19157 npadvmod Massachusetts,Millipore
R5674 T19162 T19157 punct ", ",Millipore
R5675 T19163 T19164 compound United,States
R5676 T19164 T19157 npadvmod States,Millipore
R5677 T19165 T19157 punct ),Millipore
R5678 T19166 T19149 punct .,placed
R5679 T19168 T19169 npadvmod NT,loaded
R5680 T19169 T19173 amod loaded,beads
R5681 T19170 T19168 punct -,NT
R5682 T19171 T19168 nummod 3,NT
R5683 T19172 T19169 punct -,loaded
R5684 T19173 T19189 nsubjpass beads,prepared
R5685 T19174 T19175 punct (,15
R5686 T19175 T19169 parataxis 15,loaded
R5687 T19176 T19175 nsubj n,15
R5688 T19177 T19175 punct =,15
R5689 T19178 T19175 punct ),15
R5690 T19179 T19169 cc or,loaded
R5691 T19180 T19181 npadvmod PBS,loaded
R5692 T19181 T19169 conj loaded,loaded
R5693 T19182 T19181 punct -,loaded
R5694 T19183 T19184 punct (,6
R5695 T19184 T19181 parataxis 6,loaded
R5696 T19185 T19184 nsubj n,6
R5697 T19186 T19184 punct =,6
R5698 T19187 T19184 punct ),6
R5699 T19188 T19173 compound sepharose,beads
R5700 T19190 T19189 auxpass were,prepared
R5701 T19191 T19192 mark as,described
R5702 T19192 T19189 advcl described,prepared
R5703 T19193 T19192 advmod above,described
R5704 T19194 T19189 punct ", ",prepared
R5705 T19195 T19189 cc and,prepared
R5706 T19196 T19189 conj placed,prepared
R5707 T19197 T19196 prep in,placed
R5708 T19198 T19199 det the,midline
R5709 T19199 T19197 pobj midline,in
R5710 T19200 T19196 prep at,placed
R5711 T19201 T19202 amod mid-spinal,cord
R5712 T19202 T19203 compound cord,level
R5713 T19203 T19200 pobj level,at
R5714 T19204 T19189 punct .,prepared
R5715 T19206 T19207 nsubjpass Inserts,placed
R5716 T19208 T19207 auxpass were,placed
R5717 T19209 T19207 prep in,placed
R5718 T19210 T19211 nummod six,well
R5719 T19211 T19213 compound well,plates
R5720 T19212 T19211 punct -,well
R5721 T19213 T19209 pobj plates,in
R5722 T19214 T19207 prep with,placed
R5723 T19215 T19216 npadvmod serum,free
R5724 T19216 T19218 amod free,medium
R5725 T19217 T19216 punct -,free
R5726 T19218 T19214 pobj medium,with
R5727 T19219 T19218 prep at,medium
R5728 T19220 T19221 det the,bottom
R5729 T19221 T19219 pobj bottom,at
R5730 T19222 T19221 prep of,bottom
R5731 T19223 T19224 det the,wells
R5732 T19224 T19222 pobj wells,of
R5733 T19225 T19207 punct ", ",placed
R5734 T19226 T19207 cc and,placed
R5735 T19227 T19207 conj kept,placed
R5736 T19228 T19227 prep at,kept
R5737 T19229 T19230 nummod 33,°C
R5738 T19230 T19228 pobj °C,at
R5739 T19231 T19227 prep for,kept
R5740 T19232 T19233 nummod 3,d
R5741 T19233 T19231 pobj d,for
R5742 T19234 T19227 prep in,kept
R5743 T19235 T19236 det the,presence
R5744 T19236 T19234 pobj presence,in
R5745 T19237 T19236 prep of,presence
R5746 T19238 T19239 nummod 5,%
R5747 T19239 T19240 compound %,CO2
R5748 T19240 T19237 pobj CO2,of
R5749 T19241 T19207 punct .,placed
R5750 T19243 T19244 nsubjpass Cultures,fixed
R5751 T19245 T19244 auxpass were,fixed
R5752 T19246 T19244 advmod then,fixed
R5753 T19247 T19244 prep with,fixed
R5754 T19248 T19249 nummod 4,%
R5755 T19249 T19250 compound %,paraformaldehyde
R5756 T19250 T19247 pobj paraformaldehyde,with
R5757 T19251 T19244 prep in,fixed
R5758 T19252 T19251 pobj PBS,in
R5759 T19253 T19244 punct ", ",fixed
R5760 T19254 T19244 cc and,fixed
R5761 T19255 T19256 amod small,crystals
R5762 T19256 T19257 nsubjpass crystals,placed
R5763 T19257 T19244 conj placed,fixed
R5764 T19258 T19256 prep of,crystals
R5765 T19259 T19258 pobj DiI,of
R5766 T19260 T19257 auxpass were,placed
R5767 T19261 T19257 prep into,placed
R5768 T19262 T19263 det the,DRGs
R5769 T19263 T19261 pobj DRGs,into
R5770 T19264 T19257 punct .,placed
R5771 T19266 T19267 nsubjpass Samples,incubated
R5772 T19268 T19267 auxpass were,incubated
R5773 T19269 T19267 prep in,incubated
R5774 T19270 T19271 det a,incubator
R5775 T19271 T19269 pobj incubator,in
R5776 T19272 T19273 nummod 37,°C
R5777 T19273 T19271 compound °C,incubator
R5778 T19274 T19273 punct -,°C
R5779 T19275 T19267 punct ", ",incubated
R5780 T19276 T19267 advcl allowing,incubated
R5781 T19277 T19278 det the,dye
R5782 T19278 T19279 nsubj dye,diffuse
R5783 T19279 T19276 ccomp diffuse,allowing
R5784 T19280 T19279 aux to,diffuse
R5785 T19281 T19267 punct ", ",incubated
R5786 T19282 T19267 cc and,incubated
R5787 T19283 T19267 conj photographed,incubated
R5788 T19284 T19283 prep under,photographed
R5789 T19285 T19286 det a,microscope
R5790 T19286 T19284 pobj microscope,under
R5791 T19287 T19286 nmod Nikon,microscope
R5792 T19288 T19289 punct (,Tokyo
R5793 T19289 T19287 parataxis Tokyo,Nikon
R5794 T19290 T19289 punct ", ",Tokyo
R5795 T19291 T19289 npadvmod Japan,Tokyo
R5796 T19292 T19289 punct ),Tokyo
R5797 T19293 T19294 amod inverted,epifluoresence
R5798 T19294 T19286 compound epifluoresence,microscope
R5799 T19295 T19267 punct .,incubated