PMC:523229 / 59695-60660 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T13619 0-8 NNS denotes Sections
T13621 9-12 IN denotes for
T13622 13-33 NN denotes immunohistochemistry
T13623 34-38 VBD denotes were
T13620 39-44 VBN denotes fixed
T13624 45-47 IN denotes in
T13625 48-49 CD denotes 4
T13626 49-50 NN denotes %
T13627 51-54 NN denotes PFA
T13628 54-56 , denotes ,
T13629 56-60 RB denotes then
T13630 61-69 VBN denotes digested
T13631 70-74 IN denotes with
T13632 75-78 CD denotes 942
T13634 78-79 SYM denotes
T13633 79-84 CD denotes 2,000
T13635 85-86 NNS denotes U
T13637 86-87 SYM denotes /
T13638 87-89 NN denotes ml
T13639 90-94 NN denotes type
T13641 95-97 CD denotes IV
T13642 97-98 HYPH denotes -
T13640 98-99 NN denotes S
T13643 100-106 JJ denotes bovine
T13636 107-121 NN denotes hyaluronindase
T13644 122-123 -LRB- denotes (
T13645 123-128 NNP denotes Sigma
T13646 128-130 , denotes ,
T13647 130-133 NNP denotes St.
T13648 134-139 NNP denotes Louis
T13649 139-141 , denotes ,
T13650 141-149 NNP denotes Missouri
T13651 149-151 , denotes ,
T13652 151-157 NNP denotes United
T13653 158-164 NNP denotes States
T13654 164-165 -RRB- denotes )
T13655 166-168 IN denotes in
T13656 169-172 NNS denotes PBS
T13657 173-174 -LRB- denotes (
T13658 174-176 NN denotes pH
T13659 177-178 CD denotes 5
T13660 178-179 -RRB- denotes )
T13661 180-182 IN denotes at
T13662 183-185 CD denotes 37
T13663 186-188 NN denotes °C
T13664 189-192 IN denotes for
T13665 193-195 CD denotes 30
T13666 196-199 NN denotes min
T13667 200-202 IN denotes to
T13668 203-204 CD denotes 2
T13669 205-206 NN denotes h
T13670 207-216 VBG denotes depending
T13671 217-219 IN denotes on
T13672 220-223 DT denotes the
T13673 224-229 NN denotes stage
T13674 229-230 . denotes .
T13675 230-528 sentence denotes Slides were then washed in PBS, treated with 0.3% hydrogen peroxide in 100% methanol for 30 min, washed, blocked with PBS + 0.05% Tween20 + 5% goat or fetal bovine serum, washed again, and incubated with primary antibodies in PBS + 0.05% Tween 20 + 1% goat or fetal bovine serum overnight at 4 °C.
T13676 231-237 NNS denotes Slides
T13678 238-242 VBD denotes were
T13679 243-247 RB denotes then
T13677 248-254 VBN denotes washed
T13680 255-257 IN denotes in
T13681 258-261 NN denotes PBS
T13682 261-263 , denotes ,
T13683 263-270 VBN denotes treated
T13684 271-275 IN denotes with
T13685 276-279 CD denotes 0.3
T13686 279-280 NN denotes %
T13688 281-289 NN denotes hydrogen
T13687 290-298 NN denotes peroxide
T13689 299-301 IN denotes in
T13690 302-305 CD denotes 100
T13691 305-306 NN denotes %
T13692 307-315 NN denotes methanol
T13693 316-319 IN denotes for
T13694 320-322 CD denotes 30
T13695 323-326 NN denotes min
T13696 326-328 , denotes ,
T13697 328-334 VBN denotes washed
T13698 334-336 , denotes ,
T13699 336-343 VBN denotes blocked
T13700 344-348 IN denotes with
T13701 349-352 NN denotes PBS
T13702 353-354 SYM denotes +
T13703 355-359 CD denotes 0.05
T13704 359-360 NN denotes %
T13705 361-368 NN denotes Tween20
T13706 369-370 SYM denotes +
T13707 371-372 CD denotes 5
T13708 372-373 NN denotes %
T13710 374-378 NN denotes goat
T13711 379-381 CC denotes or
T13712 382-387 JJ denotes fetal
T13713 388-394 JJ denotes bovine
T13709 395-400 NN denotes serum
T13714 400-402 , denotes ,
T13715 402-408 VBD denotes washed
T13716 409-414 RB denotes again
T13717 414-416 , denotes ,
T13718 416-419 CC denotes and
T13719 420-429 VBN denotes incubated
T13720 430-434 IN denotes with
T13721 435-442 JJ denotes primary
T13722 443-453 NNS denotes antibodies
T13723 454-456 IN denotes in
T13724 457-460 NN denotes PBS
T13725 461-462 SYM denotes +
T13726 463-467 CD denotes 0.05
T13727 467-468 NN denotes %
T13728 469-474 NN denotes Tween
T13729 475-477 CD denotes 20
T13730 478-479 SYM denotes +
T13731 480-481 CD denotes 1
T13732 481-482 NN denotes %
T13734 483-487 NN denotes goat
T13735 488-490 CC denotes or
T13736 491-496 JJ denotes fetal
T13737 497-503 JJ denotes bovine
T13733 504-509 NN denotes serum
T13738 510-519 RB denotes overnight
T13739 520-522 IN denotes at
T13740 523-524 CD denotes 4
T13741 525-527 NN denotes °C
T13742 527-528 . denotes .
T13743 528-694 sentence denotes Biotin-labeled secondary antibodies (Vector Labs) were tagged with HRP using the Vectastain Elite ABC kit (Vector Labs) followed by detection with DAB (Vector Labs).
T13744 529-535 NN denotes Biotin
T13746 535-536 HYPH denotes -
T13745 536-543 VBN denotes labeled
T13748 544-553 JJ denotes secondary
T13747 554-564 NNS denotes antibodies
T13750 565-566 -LRB- denotes (
T13752 566-572 NN denotes Vector
T13751 573-577 NNS denotes Labs
T13753 577-578 -RRB- denotes )
T13754 579-583 VBD denotes were
T13749 584-590 VBN denotes tagged
T13755 591-595 IN denotes with
T13756 596-599 NN denotes HRP
T13757 600-605 VBG denotes using
T13758 606-609 DT denotes the
T13760 610-620 NNP denotes Vectastain
T13761 621-626 NNP denotes Elite
T13762 627-630 NNP denotes ABC
T13759 631-634 NN denotes kit
T13763 635-636 -LRB- denotes (
T13765 636-642 NNP denotes Vector
T13764 643-647 NNPS denotes Labs
T13766 647-648 -RRB- denotes )
T13767 649-657 VBN denotes followed
T13768 658-660 IN denotes by
T13769 661-670 NN denotes detection
T13770 671-675 IN denotes with
T13771 676-679 NN denotes DAB
T13772 680-681 -LRB- denotes (
T13774 681-687 NNP denotes Vector
T13773 688-692 NNPS denotes Labs
T13775 692-693 -RRB- denotes )
T13776 693-694 . denotes .
T13777 694-965 sentence denotes Primary antibodies and dilutions used were: goat anti-mouse MMP13, 1:100 (Chemicon International, Temecula, California, United States); rabbit anti-human SOX9, 1:500 (Morais da Silva et al. 1996); rabbit anti-phosphorylated-SOX9 (SOX9.P), 1:10–1:250 (Huang et al. 2000).
T13778 695-702 JJ denotes Primary
T13779 703-713 NNS denotes antibodies
T13781 714-717 CC denotes and
T13782 718-727 NNS denotes dilutions
T13783 728-732 VBN denotes used
T13780 733-737 VBD denotes were
T13784 737-739 : denotes :
T13785 739-743 NN denotes goat
T13787 744-754 JJ denotes anti-mouse
T13786 755-760 NN denotes MMP13
T13788 760-762 , denotes ,
T13789 762-763 CD denotes 1
T13790 763-764 SYM denotes :
T13791 764-767 CD denotes 100
T13792 768-769 -LRB- denotes (
T13794 769-777 NNP denotes Chemicon
T13793 778-791 NNP denotes International
T13795 791-793 , denotes ,
T13796 793-801 NNP denotes Temecula
T13797 801-803 , denotes ,
T13798 803-813 NNP denotes California
T13799 813-815 , denotes ,
T13800 815-821 NNP denotes United
T13801 822-828 NNP denotes States
T13802 828-829 -RRB- denotes )
T13803 829-830 : denotes ;
T13804 831-837 NN denotes rabbit
T13806 838-848 JJ denotes anti-human
T13805 849-853 NN denotes SOX9
T13807 853-855 , denotes ,
T13808 855-856 CD denotes 1
T13809 856-857 SYM denotes :
T13810 857-860 CD denotes 500
T13811 861-862 -LRB- denotes (
T13812 862-868 NNP denotes Morais
T13813 869-871 NNP denotes da
T13814 872-877 NNP denotes Silva
T13815 878-880 FW denotes et
T13816 881-884 FW denotes al.
T13817 885-889 CD denotes 1996
T13818 889-890 -RRB- denotes )
T13819 890-891 : denotes ;
T13820 892-898 NN denotes rabbit
T13822 899-918 VBN denotes anti-phosphorylated
T13823 918-919 HYPH denotes -
T13821 919-923 NN denotes SOX9
T13824 924-925 -LRB- denotes (
T13825 925-929 NN denotes SOX9
T13827 929-930 , denotes .
T13826 930-931 NN denotes P
T13828 931-932 -RRB- denotes )
T13829 932-934 , denotes ,
T13830 934-935 CD denotes 1
T13831 935-936 SYM denotes :
T13832 936-938 CD denotes 10
T13833 938-939 SYM denotes
T13834 939-940 CD denotes 1
T13835 940-941 SYM denotes :
T13836 941-944 CD denotes 250
T13837 945-946 -LRB- denotes (
T13838 946-951 NNP denotes Huang
T13839 952-954 FW denotes et
T13840 955-958 FW denotes al.
T13841 959-963 CD denotes 2000
T13842 963-964 -RRB- denotes )
T13843 964-965 . denotes .
R9060 T13619 T13620 nsubjpass Sections,fixed
R9061 T13621 T13619 prep for,Sections
R9062 T13622 T13621 pobj immunohistochemistry,for
R9063 T13623 T13620 auxpass were,fixed
R9064 T13624 T13620 prep in,fixed
R9065 T13625 T13626 nummod 4,%
R9066 T13626 T13627 compound %,PFA
R9067 T13627 T13624 pobj PFA,in
R9068 T13628 T13620 punct ", ",fixed
R9069 T13629 T13630 advmod then,digested
R9070 T13630 T13620 dep digested,fixed
R9071 T13631 T13630 prep with,digested
R9072 T13632 T13633 quantmod 942,"2,000"
R9073 T13633 T13635 nummod "2,000",U
R9074 T13634 T13633 punct –,"2,000"
R9075 T13635 T13636 nmod U,hyaluronindase
R9076 T13636 T13631 pobj hyaluronindase,with
R9077 T13637 T13638 punct /,ml
R9078 T13638 T13635 prep ml,U
R9079 T13639 T13640 nmod type,S
R9080 T13640 T13636 nmod S,hyaluronindase
R9081 T13641 T13640 nummod IV,S
R9082 T13642 T13640 punct -,S
R9083 T13643 T13636 amod bovine,hyaluronindase
R9084 T13644 T13645 punct (,Sigma
R9085 T13645 T13636 parataxis Sigma,hyaluronindase
R9086 T13646 T13645 punct ", ",Sigma
R9087 T13647 T13648 compound St.,Louis
R9088 T13648 T13645 npadvmod Louis,Sigma
R9089 T13649 T13645 punct ", ",Sigma
R9090 T13650 T13645 npadvmod Missouri,Sigma
R9091 T13651 T13645 punct ", ",Sigma
R9092 T13652 T13653 compound United,States
R9093 T13653 T13645 npadvmod States,Sigma
R9094 T13654 T13645 punct ),Sigma
R9095 T13655 T13630 prep in,digested
R9096 T13656 T13655 pobj PBS,in
R9097 T13657 T13656 punct (,PBS
R9098 T13658 T13656 npadvmod pH,PBS
R9099 T13659 T13658 nummod 5,pH
R9100 T13660 T13656 punct ),PBS
R9101 T13661 T13630 prep at,digested
R9102 T13662 T13663 nummod 37,°C
R9103 T13663 T13661 pobj °C,at
R9104 T13664 T13630 prep for,digested
R9105 T13665 T13666 nummod 30,min
R9106 T13666 T13664 pobj min,for
R9107 T13667 T13666 prep to,min
R9108 T13668 T13669 nummod 2,h
R9109 T13669 T13667 pobj h,to
R9110 T13670 T13666 prep depending,min
R9111 T13671 T13670 prep on,depending
R9112 T13672 T13673 det the,stage
R9113 T13673 T13671 pobj stage,on
R9114 T13674 T13620 punct .,fixed
R9115 T13676 T13677 nsubjpass Slides,washed
R9116 T13678 T13677 auxpass were,washed
R9117 T13679 T13677 advmod then,washed
R9118 T13680 T13677 prep in,washed
R9119 T13681 T13680 pobj PBS,in
R9120 T13682 T13677 punct ", ",washed
R9121 T13683 T13677 conj treated,washed
R9122 T13684 T13683 prep with,treated
R9123 T13685 T13686 nummod 0.3,%
R9124 T13686 T13687 compound %,peroxide
R9125 T13687 T13684 pobj peroxide,with
R9126 T13688 T13687 compound hydrogen,peroxide
R9127 T13689 T13683 prep in,treated
R9128 T13690 T13691 nummod 100,%
R9129 T13691 T13692 compound %,methanol
R9130 T13692 T13689 pobj methanol,in
R9131 T13693 T13683 prep for,treated
R9132 T13694 T13695 nummod 30,min
R9133 T13695 T13693 pobj min,for
R9134 T13696 T13683 punct ", ",treated
R9135 T13697 T13683 conj washed,treated
R9136 T13698 T13697 punct ", ",washed
R9137 T13699 T13697 conj blocked,washed
R9138 T13700 T13699 prep with,blocked
R9139 T13701 T13700 pobj PBS,with
R9140 T13702 T13701 punct +,PBS
R9141 T13703 T13704 nummod 0.05,%
R9142 T13704 T13705 compound %,Tween20
R9143 T13705 T13701 appos Tween20,PBS
R9144 T13706 T13701 punct +,PBS
R9145 T13707 T13708 nummod 5,%
R9146 T13708 T13709 nmod %,serum
R9147 T13709 T13701 appos serum,PBS
R9148 T13710 T13709 nmod goat,serum
R9149 T13711 T13710 cc or,goat
R9150 T13712 T13713 amod fetal,bovine
R9151 T13713 T13710 conj bovine,goat
R9152 T13714 T13699 punct ", ",blocked
R9153 T13715 T13699 conj washed,blocked
R9154 T13716 T13715 advmod again,washed
R9155 T13717 T13715 punct ", ",washed
R9156 T13718 T13715 cc and,washed
R9157 T13719 T13715 conj incubated,washed
R9158 T13720 T13719 prep with,incubated
R9159 T13721 T13722 amod primary,antibodies
R9160 T13722 T13720 pobj antibodies,with
R9161 T13723 T13719 prep in,incubated
R9162 T13724 T13723 pobj PBS,in
R9163 T13725 T13724 punct +,PBS
R9164 T13726 T13727 nummod 0.05,%
R9165 T13727 T13728 compound %,Tween
R9166 T13728 T13724 appos Tween,PBS
R9167 T13729 T13728 nummod 20,Tween
R9168 T13730 T13724 punct +,PBS
R9169 T13731 T13732 nummod 1,%
R9170 T13732 T13733 nmod %,serum
R9171 T13733 T13724 appos serum,PBS
R9172 T13734 T13733 nmod goat,serum
R9173 T13735 T13734 cc or,goat
R9174 T13736 T13737 amod fetal,bovine
R9175 T13737 T13734 conj bovine,goat
R9176 T13738 T13719 advmod overnight,incubated
R9177 T13739 T13719 prep at,incubated
R9178 T13740 T13741 nummod 4,°C
R9179 T13741 T13739 pobj °C,at
R9180 T13742 T13677 punct .,washed
R9181 T13744 T13745 npadvmod Biotin,labeled
R9182 T13745 T13747 amod labeled,antibodies
R9183 T13746 T13745 punct -,labeled
R9184 T13747 T13749 nsubjpass antibodies,tagged
R9185 T13748 T13747 amod secondary,antibodies
R9186 T13750 T13751 punct (,Labs
R9187 T13751 T13747 parataxis Labs,antibodies
R9188 T13752 T13751 compound Vector,Labs
R9189 T13753 T13751 punct ),Labs
R9190 T13754 T13749 auxpass were,tagged
R9191 T13755 T13749 prep with,tagged
R9192 T13756 T13755 pobj HRP,with
R9193 T13757 T13749 advcl using,tagged
R9194 T13758 T13759 det the,kit
R9195 T13759 T13757 dobj kit,using
R9196 T13760 T13761 compound Vectastain,Elite
R9197 T13761 T13759 compound Elite,kit
R9198 T13762 T13759 compound ABC,kit
R9199 T13763 T13764 punct (,Labs
R9200 T13764 T13759 parataxis Labs,kit
R9201 T13765 T13764 compound Vector,Labs
R9202 T13766 T13764 punct ),Labs
R9203 T13767 T13749 advcl followed,tagged
R9204 T13768 T13767 agent by,followed
R9205 T13769 T13768 pobj detection,by
R9206 T13770 T13769 prep with,detection
R9207 T13771 T13770 pobj DAB,with
R9208 T13772 T13773 punct (,Labs
R9209 T13773 T13771 parataxis Labs,DAB
R9210 T13774 T13773 compound Vector,Labs
R9211 T13775 T13773 punct ),Labs
R9212 T13776 T13749 punct .,tagged
R9213 T13778 T13779 amod Primary,antibodies
R9214 T13779 T13780 nsubj antibodies,were
R9215 T13781 T13779 cc and,antibodies
R9216 T13782 T13779 conj dilutions,antibodies
R9217 T13783 T13779 acl used,antibodies
R9218 T13784 T13780 punct : ,were
R9219 T13785 T13786 nmod goat,MMP13
R9220 T13786 T13780 attr MMP13,were
R9221 T13787 T13785 amod anti-mouse,goat
R9222 T13788 T13786 punct ", ",MMP13
R9223 T13789 T13786 npadvmod 1,MMP13
R9224 T13790 T13791 punct :,100
R9225 T13791 T13789 prep 100,1
R9226 T13792 T13793 punct (,International
R9227 T13793 T13789 parataxis International,1
R9228 T13794 T13793 compound Chemicon,International
R9229 T13795 T13793 punct ", ",International
R9230 T13796 T13793 npadvmod Temecula,International
R9231 T13797 T13793 punct ", ",International
R9232 T13798 T13793 npadvmod California,International
R9233 T13799 T13793 punct ", ",International
R9234 T13800 T13801 compound United,States
R9235 T13801 T13793 npadvmod States,International
R9236 T13802 T13793 punct ),International
R9237 T13803 T13786 punct ;,MMP13
R9238 T13804 T13805 nmod rabbit,SOX9
R9239 T13805 T13786 appos SOX9,MMP13
R9240 T13806 T13804 amod anti-human,rabbit
R9241 T13807 T13805 punct ", ",SOX9
R9242 T13808 T13805 npadvmod 1,SOX9
R9243 T13809 T13810 punct :,500
R9244 T13810 T13808 prep 500,1
R9245 T13811 T13812 punct (,Morais
R9246 T13812 T13808 meta Morais,1
R9247 T13813 T13812 nmod da,Morais
R9248 T13814 T13812 nmod Silva,Morais
R9249 T13815 T13812 nmod et,Morais
R9250 T13816 T13812 nmod al.,Morais
R9251 T13817 T13812 nummod 1996,Morais
R9252 T13818 T13812 punct ),Morais
R9253 T13819 T13786 punct ;,MMP13
R9254 T13820 T13821 nmod rabbit,SOX9
R9255 T13821 T13786 appos SOX9,MMP13
R9256 T13822 T13821 amod anti-phosphorylated,SOX9
R9257 T13823 T13821 punct -,SOX9
R9258 T13824 T13821 punct (,SOX9
R9259 T13825 T13826 nmod SOX9,P
R9260 T13826 T13821 appos P,SOX9
R9261 T13827 T13826 punct .,P
R9262 T13828 T13821 punct ),SOX9
R9263 T13829 T13821 punct ", ",SOX9
R9264 T13830 T13821 npadvmod 1,SOX9
R9265 T13831 T13832 punct :,10
R9266 T13832 T13830 prep 10,1
R9267 T13833 T13834 punct –,1
R9268 T13834 T13830 prep 1,1
R9269 T13835 T13836 punct :,250
R9270 T13836 T13834 prep 250,1
R9271 T13837 T13838 punct (,Huang
R9272 T13838 T13780 meta Huang,were
R9273 T13839 T13838 nmod et,Huang
R9274 T13840 T13838 nmod al.,Huang
R9275 T13841 T13838 nummod 2000,Huang
R9276 T13842 T13838 punct ),Huang
R9277 T13843 T13780 punct .,were