PMC:523229 / 58607-59083 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T13385 0-476 sentence denotes RNA in situ hybridization was performed as described (Storm and Kingsley 1996), with the following modifications: (1) Prior to the acetylation step, sections were incubated with 10–20 μg/ml proteinase K for 30 s to 7 min at room temperature (depending on the developmental stage), followed by refixing in 4% PFA and washing three times in PBS; (2) prehybridization step was skipped, and (3) embryonic tissue sections used a different color development mix (Thut et al. 2001).
T13386 1-4 NN denotes RNA
T13388 5-7 FW denotes in
T13389 8-12 FW denotes situ
T13387 13-26 NN denotes hybridization
T13391 27-30 VBD denotes was
T13390 31-40 VBN denotes performed
T13392 41-43 IN denotes as
T13393 44-53 VBN denotes described
T13394 54-55 -LRB- denotes (
T13395 55-60 NNP denotes Storm
T13396 61-64 CC denotes and
T13397 65-73 NNP denotes Kingsley
T13398 74-78 CD denotes 1996
T13399 78-79 -RRB- denotes )
T13400 79-81 , denotes ,
T13401 81-85 IN denotes with
T13402 86-89 DT denotes the
T13404 90-99 VBG denotes following
T13403 100-113 NNS denotes modifications
T13405 113-115 : denotes :
T13406 115-116 -LRB- denotes (
T13407 116-117 LS denotes 1
T13409 117-118 -RRB- denotes )
T13410 119-124 JJ denotes Prior
T13411 125-127 IN denotes to
T13412 128-131 DT denotes the
T13414 132-143 NN denotes acetylation
T13413 144-148 NN denotes step
T13415 148-150 , denotes ,
T13416 150-158 NNS denotes sections
T13417 159-163 VBD denotes were
T13408 164-173 VBN denotes incubated
T13418 174-178 IN denotes with
T13419 179-181 CD denotes 10
T13421 181-182 HYPH denotes
T13420 182-184 CD denotes 20
T13422 185-187 NNS denotes μg
T13424 187-188 SYM denotes /
T13425 188-190 NN denotes ml
T13426 191-201 NN denotes proteinase
T13423 202-203 NN denotes K
T13427 204-207 IN denotes for
T13428 208-210 CD denotes 30
T13429 211-212 NN denotes s
T13430 213-215 IN denotes to
T13431 216-217 CD denotes 7
T13432 218-221 NN denotes min
T13433 222-224 IN denotes at
T13434 225-229 NN denotes room
T13435 230-241 NN denotes temperature
T13436 242-243 -LRB- denotes (
T13437 243-252 VBG denotes depending
T13438 253-255 IN denotes on
T13439 256-259 DT denotes the
T13441 260-273 JJ denotes developmental
T13440 274-279 NN denotes stage
T13442 279-280 -RRB- denotes )
T13443 280-282 , denotes ,
T13444 282-290 VBN denotes followed
T13445 291-293 IN denotes by
T13446 294-302 VBG denotes refixing
T13447 303-305 IN denotes in
T13448 306-307 CD denotes 4
T13449 307-308 NN denotes %
T13450 309-312 NN denotes PFA
T13451 313-316 CC denotes and
T13452 317-324 VBG denotes washing
T13453 325-330 CD denotes three
T13454 331-336 NNS denotes times
T13455 337-339 IN denotes in
T13456 340-343 NN denotes PBS
T13457 343-344 : denotes ;
T13458 345-346 -LRB- denotes (
T13459 346-347 LS denotes 2
T13461 347-348 -RRB- denotes )
T13462 349-365 NN denotes prehybridization
T13463 366-370 NN denotes step
T13464 371-374 VBD denotes was
T13460 375-382 VBN denotes skipped
T13465 382-384 , denotes ,
T13466 384-387 CC denotes and
T13467 388-389 -LRB- denotes (
T13468 389-390 LS denotes 3
T13470 390-391 -RRB- denotes )
T13471 392-401 JJ denotes embryonic
T13472 402-408 NN denotes tissue
T13473 409-417 NNS denotes sections
T13469 418-422 VBD denotes used
T13474 423-424 DT denotes a
T13476 425-434 JJ denotes different
T13477 435-440 NN denotes color
T13478 441-452 NN denotes development
T13475 453-456 NN denotes mix
T13479 457-458 -LRB- denotes (
T13480 458-462 NNP denotes Thut
T13481 463-465 FW denotes et
T13482 466-469 FW denotes al.
T13483 470-474 CD denotes 2001
T13484 474-475 -RRB- denotes )
T13485 475-476 . denotes .
R8833 T13386 T13387 nmod RNA,hybridization
R8834 T13387 T13390 nsubjpass hybridization,performed
R8835 T13388 T13389 advmod in,situ
R8836 T13389 T13387 amod situ,hybridization
R8837 T13391 T13390 auxpass was,performed
R8838 T13392 T13393 mark as,described
R8839 T13393 T13390 advcl described,performed
R8840 T13394 T13395 punct (,Storm
R8841 T13395 T13393 meta Storm,described
R8842 T13396 T13395 cc and,Storm
R8843 T13397 T13395 conj Kingsley,Storm
R8844 T13398 T13397 nummod 1996,Kingsley
R8845 T13399 T13397 punct ),Kingsley
R8846 T13400 T13390 punct ", ",performed
R8847 T13401 T13390 prep with,performed
R8848 T13402 T13403 det the,modifications
R8849 T13403 T13401 pobj modifications,with
R8850 T13404 T13403 amod following,modifications
R8851 T13405 T13390 punct : ,performed
R8852 T13406 T13407 punct (,1
R8853 T13407 T13408 meta 1,incubated
R8854 T13408 T13390 conj incubated,performed
R8855 T13409 T13407 punct ),1
R8856 T13410 T13411 amod Prior,to
R8857 T13411 T13408 prep to,incubated
R8858 T13412 T13413 det the,step
R8859 T13413 T13411 pobj step,to
R8860 T13414 T13413 compound acetylation,step
R8861 T13415 T13408 punct ", ",incubated
R8862 T13416 T13408 nsubjpass sections,incubated
R8863 T13417 T13408 auxpass were,incubated
R8864 T13418 T13408 prep with,incubated
R8865 T13419 T13420 compound 10,20
R8866 T13420 T13422 nummod 20,μg
R8867 T13421 T13420 punct –,20
R8868 T13422 T13423 nmod μg,K
R8869 T13423 T13418 pobj K,with
R8870 T13424 T13425 punct /,ml
R8871 T13425 T13422 prep ml,μg
R8872 T13426 T13423 compound proteinase,K
R8873 T13427 T13408 prep for,incubated
R8874 T13428 T13429 nummod 30,s
R8875 T13429 T13427 pobj s,for
R8876 T13430 T13429 prep to,s
R8877 T13431 T13432 nummod 7,min
R8878 T13432 T13430 pobj min,to
R8879 T13433 T13408 prep at,incubated
R8880 T13434 T13435 compound room,temperature
R8881 T13435 T13433 pobj temperature,at
R8882 T13436 T13435 punct (,temperature
R8883 T13437 T13435 prep depending,temperature
R8884 T13438 T13437 prep on,depending
R8885 T13439 T13440 det the,stage
R8886 T13440 T13438 pobj stage,on
R8887 T13441 T13440 amod developmental,stage
R8888 T13442 T13435 punct ),temperature
R8889 T13443 T13408 punct ", ",incubated
R8890 T13444 T13408 advcl followed,incubated
R8891 T13445 T13444 agent by,followed
R8892 T13446 T13445 pcomp refixing,by
R8893 T13447 T13446 prep in,refixing
R8894 T13448 T13449 nummod 4,%
R8895 T13449 T13450 compound %,PFA
R8896 T13450 T13447 pobj PFA,in
R8897 T13451 T13446 cc and,refixing
R8898 T13452 T13446 conj washing,refixing
R8899 T13453 T13454 nummod three,times
R8900 T13454 T13452 npadvmod times,washing
R8901 T13455 T13452 prep in,washing
R8902 T13456 T13455 pobj PBS,in
R8903 T13457 T13408 punct ;,incubated
R8904 T13458 T13459 punct (,2
R8905 T13459 T13460 meta 2,skipped
R8906 T13460 T13408 conj skipped,incubated
R8907 T13461 T13459 punct ),2
R8908 T13462 T13463 compound prehybridization,step
R8909 T13463 T13460 nsubjpass step,skipped
R8910 T13464 T13460 auxpass was,skipped
R8911 T13465 T13460 punct ", ",skipped
R8912 T13466 T13460 cc and,skipped
R8913 T13467 T13468 punct (,3
R8914 T13468 T13469 meta 3,used
R8915 T13469 T13460 conj used,skipped
R8916 T13470 T13468 punct ),3
R8917 T13471 T13472 amod embryonic,tissue
R8918 T13472 T13473 compound tissue,sections
R8919 T13473 T13469 nsubj sections,used
R8920 T13474 T13475 det a,mix
R8921 T13475 T13469 dobj mix,used
R8922 T13476 T13475 amod different,mix
R8923 T13477 T13475 compound color,mix
R8924 T13478 T13475 compound development,mix
R8925 T13479 T13480 punct (,Thut
R8926 T13480 T13469 meta Thut,used
R8927 T13481 T13480 nmod et,Thut
R8928 T13482 T13480 nmod al.,Thut
R8929 T13483 T13480 nummod 2001,Thut
R8930 T13484 T13480 punct ),Thut
R8931 T13485 T13469 punct .,used