PMC:523229 / 58607-59083 5 Projects
Annnotations
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T13385 | 0-476 | sentence | denotes | RNA in situ hybridization was performed as described (Storm and Kingsley 1996), with the following modifications: (1) Prior to the acetylation step, sections were incubated with 10–20 μg/ml proteinase K for 30 s to 7 min at room temperature (depending on the developmental stage), followed by refixing in 4% PFA and washing three times in PBS; (2) prehybridization step was skipped, and (3) embryonic tissue sections used a different color development mix (Thut et al. 2001). |
T13386 | 1-4 | NN | denotes | RNA |
T13388 | 5-7 | FW | denotes | in |
T13389 | 8-12 | FW | denotes | situ |
T13387 | 13-26 | NN | denotes | hybridization |
T13391 | 27-30 | VBD | denotes | was |
T13390 | 31-40 | VBN | denotes | performed |
T13392 | 41-43 | IN | denotes | as |
T13393 | 44-53 | VBN | denotes | described |
T13394 | 54-55 | -LRB- | denotes | ( |
T13395 | 55-60 | NNP | denotes | Storm |
T13396 | 61-64 | CC | denotes | and |
T13397 | 65-73 | NNP | denotes | Kingsley |
T13398 | 74-78 | CD | denotes | 1996 |
T13399 | 78-79 | -RRB- | denotes | ) |
T13400 | 79-81 | , | denotes | , |
T13401 | 81-85 | IN | denotes | with |
T13402 | 86-89 | DT | denotes | the |
T13404 | 90-99 | VBG | denotes | following |
T13403 | 100-113 | NNS | denotes | modifications |
T13405 | 113-115 | : | denotes | : |
T13406 | 115-116 | -LRB- | denotes | ( |
T13407 | 116-117 | LS | denotes | 1 |
T13409 | 117-118 | -RRB- | denotes | ) |
T13410 | 119-124 | JJ | denotes | Prior |
T13411 | 125-127 | IN | denotes | to |
T13412 | 128-131 | DT | denotes | the |
T13414 | 132-143 | NN | denotes | acetylation |
T13413 | 144-148 | NN | denotes | step |
T13415 | 148-150 | , | denotes | , |
T13416 | 150-158 | NNS | denotes | sections |
T13417 | 159-163 | VBD | denotes | were |
T13408 | 164-173 | VBN | denotes | incubated |
T13418 | 174-178 | IN | denotes | with |
T13419 | 179-181 | CD | denotes | 10 |
T13421 | 181-182 | HYPH | denotes | – |
T13420 | 182-184 | CD | denotes | 20 |
T13422 | 185-187 | NNS | denotes | μg |
T13424 | 187-188 | SYM | denotes | / |
T13425 | 188-190 | NN | denotes | ml |
T13426 | 191-201 | NN | denotes | proteinase |
T13423 | 202-203 | NN | denotes | K |
T13427 | 204-207 | IN | denotes | for |
T13428 | 208-210 | CD | denotes | 30 |
T13429 | 211-212 | NN | denotes | s |
T13430 | 213-215 | IN | denotes | to |
T13431 | 216-217 | CD | denotes | 7 |
T13432 | 218-221 | NN | denotes | min |
T13433 | 222-224 | IN | denotes | at |
T13434 | 225-229 | NN | denotes | room |
T13435 | 230-241 | NN | denotes | temperature |
T13436 | 242-243 | -LRB- | denotes | ( |
T13437 | 243-252 | VBG | denotes | depending |
T13438 | 253-255 | IN | denotes | on |
T13439 | 256-259 | DT | denotes | the |
T13441 | 260-273 | JJ | denotes | developmental |
T13440 | 274-279 | NN | denotes | stage |
T13442 | 279-280 | -RRB- | denotes | ) |
T13443 | 280-282 | , | denotes | , |
T13444 | 282-290 | VBN | denotes | followed |
T13445 | 291-293 | IN | denotes | by |
T13446 | 294-302 | VBG | denotes | refixing |
T13447 | 303-305 | IN | denotes | in |
T13448 | 306-307 | CD | denotes | 4 |
T13449 | 307-308 | NN | denotes | % |
T13450 | 309-312 | NN | denotes | PFA |
T13451 | 313-316 | CC | denotes | and |
T13452 | 317-324 | VBG | denotes | washing |
T13453 | 325-330 | CD | denotes | three |
T13454 | 331-336 | NNS | denotes | times |
T13455 | 337-339 | IN | denotes | in |
T13456 | 340-343 | NN | denotes | PBS |
T13457 | 343-344 | : | denotes | ; |
T13458 | 345-346 | -LRB- | denotes | ( |
T13459 | 346-347 | LS | denotes | 2 |
T13461 | 347-348 | -RRB- | denotes | ) |
T13462 | 349-365 | NN | denotes | prehybridization |
T13463 | 366-370 | NN | denotes | step |
T13464 | 371-374 | VBD | denotes | was |
T13460 | 375-382 | VBN | denotes | skipped |
T13465 | 382-384 | , | denotes | , |
T13466 | 384-387 | CC | denotes | and |
T13467 | 388-389 | -LRB- | denotes | ( |
T13468 | 389-390 | LS | denotes | 3 |
T13470 | 390-391 | -RRB- | denotes | ) |
T13471 | 392-401 | JJ | denotes | embryonic |
T13472 | 402-408 | NN | denotes | tissue |
T13473 | 409-417 | NNS | denotes | sections |
T13469 | 418-422 | VBD | denotes | used |
T13474 | 423-424 | DT | denotes | a |
T13476 | 425-434 | JJ | denotes | different |
T13477 | 435-440 | NN | denotes | color |
T13478 | 441-452 | NN | denotes | development |
T13475 | 453-456 | NN | denotes | mix |
T13479 | 457-458 | -LRB- | denotes | ( |
T13480 | 458-462 | NNP | denotes | Thut |
T13481 | 463-465 | FW | denotes | et |
T13482 | 466-469 | FW | denotes | al. |
T13483 | 470-474 | CD | denotes | 2001 |
T13484 | 474-475 | -RRB- | denotes | ) |
T13485 | 475-476 | . | denotes | . |
R8833 | T13386 | T13387 | nmod | RNA,hybridization |
R8834 | T13387 | T13390 | nsubjpass | hybridization,performed |
R8835 | T13388 | T13389 | advmod | in,situ |
R8836 | T13389 | T13387 | amod | situ,hybridization |
R8837 | T13391 | T13390 | auxpass | was,performed |
R8838 | T13392 | T13393 | mark | as,described |
R8839 | T13393 | T13390 | advcl | described,performed |
R8840 | T13394 | T13395 | punct | (,Storm |
R8841 | T13395 | T13393 | meta | Storm,described |
R8842 | T13396 | T13395 | cc | and,Storm |
R8843 | T13397 | T13395 | conj | Kingsley,Storm |
R8844 | T13398 | T13397 | nummod | 1996,Kingsley |
R8845 | T13399 | T13397 | punct | ),Kingsley |
R8846 | T13400 | T13390 | punct | ", ",performed |
R8847 | T13401 | T13390 | prep | with,performed |
R8848 | T13402 | T13403 | det | the,modifications |
R8849 | T13403 | T13401 | pobj | modifications,with |
R8850 | T13404 | T13403 | amod | following,modifications |
R8851 | T13405 | T13390 | punct | : ,performed |
R8852 | T13406 | T13407 | punct | (,1 |
R8853 | T13407 | T13408 | meta | 1,incubated |
R8854 | T13408 | T13390 | conj | incubated,performed |
R8855 | T13409 | T13407 | punct | ),1 |
R8856 | T13410 | T13411 | amod | Prior,to |
R8857 | T13411 | T13408 | prep | to,incubated |
R8858 | T13412 | T13413 | det | the,step |
R8859 | T13413 | T13411 | pobj | step,to |
R8860 | T13414 | T13413 | compound | acetylation,step |
R8861 | T13415 | T13408 | punct | ", ",incubated |
R8862 | T13416 | T13408 | nsubjpass | sections,incubated |
R8863 | T13417 | T13408 | auxpass | were,incubated |
R8864 | T13418 | T13408 | prep | with,incubated |
R8865 | T13419 | T13420 | compound | 10,20 |
R8866 | T13420 | T13422 | nummod | 20,μg |
R8867 | T13421 | T13420 | punct | –,20 |
R8868 | T13422 | T13423 | nmod | μg,K |
R8869 | T13423 | T13418 | pobj | K,with |
R8870 | T13424 | T13425 | punct | /,ml |
R8871 | T13425 | T13422 | prep | ml,μg |
R8872 | T13426 | T13423 | compound | proteinase,K |
R8873 | T13427 | T13408 | prep | for,incubated |
R8874 | T13428 | T13429 | nummod | 30,s |
R8875 | T13429 | T13427 | pobj | s,for |
R8876 | T13430 | T13429 | prep | to,s |
R8877 | T13431 | T13432 | nummod | 7,min |
R8878 | T13432 | T13430 | pobj | min,to |
R8879 | T13433 | T13408 | prep | at,incubated |
R8880 | T13434 | T13435 | compound | room,temperature |
R8881 | T13435 | T13433 | pobj | temperature,at |
R8882 | T13436 | T13435 | punct | (,temperature |
R8883 | T13437 | T13435 | prep | depending,temperature |
R8884 | T13438 | T13437 | prep | on,depending |
R8885 | T13439 | T13440 | det | the,stage |
R8886 | T13440 | T13438 | pobj | stage,on |
R8887 | T13441 | T13440 | amod | developmental,stage |
R8888 | T13442 | T13435 | punct | ),temperature |
R8889 | T13443 | T13408 | punct | ", ",incubated |
R8890 | T13444 | T13408 | advcl | followed,incubated |
R8891 | T13445 | T13444 | agent | by,followed |
R8892 | T13446 | T13445 | pcomp | refixing,by |
R8893 | T13447 | T13446 | prep | in,refixing |
R8894 | T13448 | T13449 | nummod | 4,% |
R8895 | T13449 | T13450 | compound | %,PFA |
R8896 | T13450 | T13447 | pobj | PFA,in |
R8897 | T13451 | T13446 | cc | and,refixing |
R8898 | T13452 | T13446 | conj | washing,refixing |
R8899 | T13453 | T13454 | nummod | three,times |
R8900 | T13454 | T13452 | npadvmod | times,washing |
R8901 | T13455 | T13452 | prep | in,washing |
R8902 | T13456 | T13455 | pobj | PBS,in |
R8903 | T13457 | T13408 | punct | ;,incubated |
R8904 | T13458 | T13459 | punct | (,2 |
R8905 | T13459 | T13460 | meta | 2,skipped |
R8906 | T13460 | T13408 | conj | skipped,incubated |
R8907 | T13461 | T13459 | punct | ),2 |
R8908 | T13462 | T13463 | compound | prehybridization,step |
R8909 | T13463 | T13460 | nsubjpass | step,skipped |
R8910 | T13464 | T13460 | auxpass | was,skipped |
R8911 | T13465 | T13460 | punct | ", ",skipped |
R8912 | T13466 | T13460 | cc | and,skipped |
R8913 | T13467 | T13468 | punct | (,3 |
R8914 | T13468 | T13469 | meta | 3,used |
R8915 | T13469 | T13460 | conj | used,skipped |
R8916 | T13470 | T13468 | punct | ),3 |
R8917 | T13471 | T13472 | amod | embryonic,tissue |
R8918 | T13472 | T13473 | compound | tissue,sections |
R8919 | T13473 | T13469 | nsubj | sections,used |
R8920 | T13474 | T13475 | det | a,mix |
R8921 | T13475 | T13469 | dobj | mix,used |
R8922 | T13476 | T13475 | amod | different,mix |
R8923 | T13477 | T13475 | compound | color,mix |
R8924 | T13478 | T13475 | compound | development,mix |
R8925 | T13479 | T13480 | punct | (,Thut |
R8926 | T13480 | T13469 | meta | Thut,used |
R8927 | T13481 | T13480 | nmod | et,Thut |
R8928 | T13482 | T13480 | nmod | al.,Thut |
R8929 | T13483 | T13480 | nummod | 2001,Thut |
R8930 | T13484 | T13480 | punct | ),Thut |
R8931 | T13485 | T13469 | punct | .,used |