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PMC:523229 / 50578-52543 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T11321 0-1 DT denotes A
T11323 2-7 NN denotes mouse
T11325 8-13 NN denotes 129x1
T11327 13-14 HYPH denotes /
T11326 14-17 NN denotes SvJ
T11324 18-21 NN denotes BAC
T11322 22-29 NN denotes library
T11329 30-31 -LRB- denotes (
T11330 31-41 NNP denotes Invitrogen
T11331 41-42 -RRB- denotes )
T11332 43-46 VBD denotes was
T11328 47-55 VBN denotes screened
T11333 56-58 TO denotes to
T11334 59-67 VB denotes identify
T11335 68-69 DT denotes a
T11337 70-73 CD denotes 140
T11339 73-74 HYPH denotes -
T11338 74-76 NN denotes kb
T11336 77-80 NN denotes BAC
T11340 81-85 IN denotes from
T11341 86-89 DT denotes the
T11343 90-94 NN denotes Gdf5
T11342 95-100 NN denotes locus
T11344 100-101 . denotes .
T11345 101-388 sentence denotes This BAC was modified using a homologous recombination system in E. coli (Yang et al. 1997) to place nuclear-localized Cre recombinase (from plasmid pML78, gift of Gail Martin) followed by IRES-hPLAP (from plasmid 1726, gift of Oliver Bogler) directly behind the ATG start site of Gdf5.
T11346 102-106 DT denotes This
T11347 107-110 NN denotes BAC
T11349 111-114 VBD denotes was
T11348 115-123 VBN denotes modified
T11350 124-129 VBG denotes using
T11351 130-131 DT denotes a
T11353 132-142 JJ denotes homologous
T11354 143-156 NN denotes recombination
T11352 157-163 NN denotes system
T11355 164-166 IN denotes in
T11356 167-169 NNP denotes E.
T11357 170-174 NNP denotes coli
T11358 175-176 -LRB- denotes (
T11359 176-180 NNP denotes Yang
T11360 181-183 FW denotes et
T11361 184-187 FW denotes al.
T11362 188-192 CD denotes 1997
T11363 192-193 -RRB- denotes )
T11364 194-196 TO denotes to
T11365 197-202 VB denotes place
T11366 203-210 JJ denotes nuclear
T11368 210-211 HYPH denotes -
T11367 211-220 VBN denotes localized
T11370 221-224 NN denotes Cre
T11369 225-236 NN denotes recombinase
T11371 237-238 -LRB- denotes (
T11372 238-242 IN denotes from
T11373 243-250 NN denotes plasmid
T11374 251-256 NN denotes pML78
T11375 256-258 , denotes ,
T11376 258-262 NN denotes gift
T11377 263-265 IN denotes of
T11378 266-270 NNP denotes Gail
T11379 271-277 NNP denotes Martin
T11380 277-278 -RRB- denotes )
T11381 279-287 VBN denotes followed
T11382 288-290 IN denotes by
T11383 291-295 NN denotes IRES
T11385 295-296 HYPH denotes -
T11384 296-301 NN denotes hPLAP
T11386 302-303 -LRB- denotes (
T11387 303-307 IN denotes from
T11388 308-315 NN denotes plasmid
T11389 316-320 CD denotes 1726
T11390 320-322 , denotes ,
T11391 322-326 NN denotes gift
T11392 327-329 IN denotes of
T11393 330-336 NNP denotes Oliver
T11394 337-343 NNP denotes Bogler
T11395 343-344 -RRB- denotes )
T11396 345-353 RB denotes directly
T11397 354-360 IN denotes behind
T11398 361-364 DT denotes the
T11400 365-368 NN denotes ATG
T11401 369-374 NN denotes start
T11399 375-379 NN denotes site
T11402 380-382 IN denotes of
T11403 383-387 NN denotes Gdf5
T11404 387-388 . denotes .
T11405 388-509 sentence denotes In the process, 583 bp of the first exon of Gdf5 was removed and no functional GDF5 protein is predicted to be produced.
T11406 389-391 IN denotes In
T11408 392-395 DT denotes the
T11409 396-403 NN denotes process
T11410 403-405 , denotes ,
T11411 405-408 CD denotes 583
T11412 409-411 NNS denotes bp
T11413 412-414 IN denotes of
T11414 415-418 DT denotes the
T11416 419-424 JJ denotes first
T11415 425-429 NN denotes exon
T11417 430-432 IN denotes of
T11418 433-437 NN denotes Gdf5
T11419 438-441 VBD denotes was
T11407 442-449 VBN denotes removed
T11420 450-453 CC denotes and
T11421 454-456 DT denotes no
T11423 457-467 JJ denotes functional
T11424 468-472 NN denotes GDF5
T11422 473-480 NN denotes protein
T11426 481-483 VBZ denotes is
T11425 484-493 VBN denotes predicted
T11427 494-496 TO denotes to
T11429 497-499 VB denotes be
T11428 500-508 VBN denotes produced
T11430 508-509 . denotes .
T11431 509-801 sentence denotes The 5′ homology arm was subcloned from a PCR product tailed with XhoI and Bsp120I restriction sites that contains 781 bp of 5′ genomic Gdf5 sequence ending at the ATG translation start site (forward primer 5′-CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC-3′; reverse 5′-GTTTGGGCCCATCCTCTGGCCAGCCGCTG-3′).
T11432 510-513 DT denotes The
T11434 514-515 CD denotes 5
T11435 515-516 SYM denotes ′
T11436 517-525 NN denotes homology
T11433 526-529 NN denotes arm
T11438 530-533 VBD denotes was
T11437 534-543 VBN denotes subcloned
T11439 544-548 IN denotes from
T11440 549-550 DT denotes a
T11442 551-554 NN denotes PCR
T11441 555-562 NN denotes product
T11443 563-569 VBN denotes tailed
T11444 570-574 IN denotes with
T11445 575-579 NN denotes XhoI
T11447 580-583 CC denotes and
T11448 584-591 NN denotes Bsp120I
T11449 592-603 NN denotes restriction
T11446 604-609 NNS denotes sites
T11450 610-614 WDT denotes that
T11451 615-623 VBZ denotes contains
T11452 624-627 CD denotes 781
T11453 628-630 NNS denotes bp
T11454 631-633 IN denotes of
T11455 634-635 CD denotes 5
T11457 635-636 SYM denotes ′
T11458 637-644 JJ denotes genomic
T11459 645-649 NN denotes Gdf5
T11456 650-658 NN denotes sequence
T11460 659-665 VBG denotes ending
T11461 666-668 IN denotes at
T11462 669-672 DT denotes the
T11464 673-676 NN denotes ATG
T11465 677-688 NN denotes translation
T11466 689-694 NN denotes start
T11463 695-699 NN denotes site
T11467 700-701 -LRB- denotes (
T11469 701-708 JJ denotes forward
T11470 709-715 NN denotes primer
T11472 716-717 CD denotes 5
T11473 717-718 SYM denotes ′
T11474 718-719 HYPH denotes -
T11471 719-751 NN denotes CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
T11475 751-752 HYPH denotes -
T11476 752-753 CD denotes 3
T11477 753-754 SYM denotes ′
T11478 754-755 : denotes ;
T11479 756-763 JJ denotes reverse
T11480 764-765 CD denotes 5
T11481 765-766 SYM denotes ′
T11482 766-767 HYPH denotes -
T11468 767-796 NN denotes GTTTGGGCCCATCCTCTGGCCAGCCGCTG
T11483 796-797 HYPH denotes -
T11484 797-798 CD denotes 3
T11485 798-799 SYM denotes ′
T11486 799-800 -RRB- denotes )
T11487 800-801 . denotes .
T11488 801-866 sentence denotes Cre was subcloned from a 1.1-kb Bsp120I/EcoRI fragment of pML78.
T11489 802-805 NN denotes Cre
T11491 806-809 VBD denotes was
T11490 810-819 VBN denotes subcloned
T11492 820-824 IN denotes from
T11493 825-826 DT denotes a
T11495 827-830 CD denotes 1.1
T11497 830-831 HYPH denotes -
T11496 831-833 NN denotes kb
T11498 834-841 NN denotes Bsp120I
T11500 841-842 HYPH denotes /
T11499 842-847 NN denotes EcoRI
T11494 848-856 NN denotes fragment
T11501 857-859 IN denotes of
T11502 860-865 NN denotes pML78
T11503 865-866 . denotes .
T11504 866-1113 sentence denotes IRES hPLAP was subcloned from a 2.1-kb PCR product tailed with EcoRI and SpeI sites that contains the hPLAP translation stop site (forward primer 5′-ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG-3′; reverse 5′-AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG-3′).
T11505 867-871 NN denotes IRES
T11506 872-877 NN denotes hPLAP
T11508 878-881 VBD denotes was
T11507 882-891 VBN denotes subcloned
T11509 892-896 IN denotes from
T11510 897-898 DT denotes a
T11512 899-902 CD denotes 2.1
T11514 902-903 HYPH denotes -
T11513 903-905 NN denotes kb
T11515 906-909 NN denotes PCR
T11511 910-917 NN denotes product
T11516 918-924 VBN denotes tailed
T11517 925-929 IN denotes with
T11518 930-935 NN denotes EcoRI
T11520 936-939 CC denotes and
T11521 940-944 NN denotes SpeI
T11519 945-950 NNS denotes sites
T11522 951-955 WDT denotes that
T11523 956-964 VBZ denotes contains
T11524 965-968 DT denotes the
T11526 969-974 NN denotes hPLAP
T11527 975-986 NN denotes translation
T11528 987-991 NN denotes stop
T11525 992-996 NN denotes site
T11529 997-998 -LRB- denotes (
T11531 998-1005 JJ denotes forward
T11532 1006-1012 NN denotes primer
T11534 1013-1014 CD denotes 5
T11535 1014-1015 SYM denotes ′
T11536 1015-1016 HYPH denotes -
T11533 1016-1054 NN denotes ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
T11537 1054-1055 HYPH denotes -
T11538 1055-1056 CD denotes 3
T11539 1056-1057 SYM denotes ′
T11540 1057-1058 : denotes ;
T11541 1059-1066 JJ denotes reverse
T11542 1067-1068 CD denotes 5
T11543 1068-1069 SYM denotes ′
T11544 1069-1070 HYPH denotes -
T11530 1070-1108 NN denotes AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
T11545 1108-1109 HYPH denotes -
T11546 1109-1110 CD denotes 3
T11547 1110-1111 SYM denotes ′
T11548 1111-1112 -RRB- denotes )
T11549 1112-1113 . denotes .
T11550 1113-1281 sentence denotes The 3′ homology arm was subcloned from a 0.8-kb PCR product amplified from a 0.9-kb XhoI Gdf5 genomic subclone containing part of the first exon and downstream intron.
T11551 1114-1117 DT denotes The
T11553 1118-1119 CD denotes 3
T11554 1119-1120 SYM denotes ′
T11555 1121-1129 NN denotes homology
T11552 1130-1133 NN denotes arm
T11557 1134-1137 VBD denotes was
T11556 1138-1147 VBN denotes subcloned
T11558 1148-1152 IN denotes from
T11559 1153-1154 DT denotes a
T11561 1155-1158 CD denotes 0.8
T11563 1158-1159 HYPH denotes -
T11562 1159-1161 NN denotes kb
T11564 1162-1165 NN denotes PCR
T11560 1166-1173 NN denotes product
T11565 1174-1183 VBN denotes amplified
T11566 1184-1188 IN denotes from
T11567 1189-1190 DT denotes a
T11569 1191-1194 CD denotes 0.9
T11571 1194-1195 HYPH denotes -
T11570 1195-1197 NN denotes kb
T11572 1198-1202 NN denotes XhoI
T11573 1203-1207 NN denotes Gdf5
T11574 1208-1215 JJ denotes genomic
T11568 1216-1224 NN denotes subclone
T11575 1225-1235 VBG denotes containing
T11576 1236-1240 NN denotes part
T11577 1241-1243 IN denotes of
T11578 1244-1247 DT denotes the
T11580 1248-1253 JJ denotes first
T11579 1254-1258 NN denotes exon
T11581 1259-1262 CC denotes and
T11582 1263-1273 JJ denotes downstream
T11583 1274-1280 NN denotes intron
T11584 1280-1281 . denotes .
T11585 1281-1596 sentence denotes The forward primer contains the 3′ end of the first exon and is tailed with a SpeI site; the reverse primer is from the T7 promoter of the vector containing the 0.9-kb subclone and flanks the intronic XhoI site (forward primer 5′-CTAAACTAGTCACCAGCTTTATTGACAAAGG-3′; reverse 5′-GATTTCTAGAGTAATACGACTCACTATAGGGC-3′).
T11586 1282-1285 DT denotes The
T11588 1286-1293 JJ denotes forward
T11587 1294-1300 NN denotes primer
T11589 1301-1309 VBZ denotes contains
T11591 1310-1313 DT denotes the
T11593 1314-1315 CD denotes 3
T11594 1315-1316 SYM denotes ′
T11592 1317-1320 NN denotes end
T11595 1321-1323 IN denotes of
T11596 1324-1327 DT denotes the
T11598 1328-1333 JJ denotes first
T11597 1334-1338 NN denotes exon
T11599 1339-1342 CC denotes and
T11600 1343-1345 VBZ denotes is
T11601 1346-1352 VBN denotes tailed
T11602 1353-1357 IN denotes with
T11603 1358-1359 DT denotes a
T11605 1360-1364 NN denotes SpeI
T11604 1365-1369 NN denotes site
T11606 1369-1370 : denotes ;
T11607 1371-1374 DT denotes the
T11609 1375-1382 JJ denotes reverse
T11608 1383-1389 NN denotes primer
T11590 1390-1392 VBZ denotes is
T11610 1393-1397 IN denotes from
T11611 1398-1401 DT denotes the
T11613 1402-1404 NN denotes T7
T11612 1405-1413 NN denotes promoter
T11614 1414-1416 IN denotes of
T11615 1417-1420 DT denotes the
T11616 1421-1427 NN denotes vector
T11617 1428-1438 VBG denotes containing
T11618 1439-1442 DT denotes the
T11620 1443-1446 CD denotes 0.9
T11622 1446-1447 HYPH denotes -
T11621 1447-1449 NN denotes kb
T11619 1450-1458 NN denotes subclone
T11623 1459-1462 CC denotes and
T11624 1463-1469 VBZ denotes flanks
T11625 1470-1473 DT denotes the
T11627 1474-1482 JJ denotes intronic
T11628 1483-1487 NN denotes XhoI
T11626 1488-1492 NN denotes site
T11629 1493-1494 -LRB- denotes (
T11631 1494-1501 JJ denotes forward
T11632 1502-1508 NN denotes primer
T11634 1509-1510 CD denotes 5
T11635 1510-1511 SYM denotes ′
T11636 1511-1512 HYPH denotes -
T11633 1512-1543 NN denotes CTAAACTAGTCACCAGCTTTATTGACAAAGG
T11637 1543-1544 HYPH denotes -
T11638 1544-1545 CD denotes 3
T11639 1545-1546 SYM denotes ′
T11640 1546-1547 : denotes ;
T11641 1548-1555 JJ denotes reverse
T11642 1556-1557 CD denotes 5
T11643 1557-1558 SYM denotes ′
T11644 1558-1559 HYPH denotes -
T11630 1559-1591 NN denotes GATTTCTAGAGTAATACGACTCACTATAGGGC
T11645 1591-1592 HYPH denotes -
T11646 1592-1593 CD denotes 3
T11647 1593-1594 SYM denotes ′
T11648 1594-1595 -RRB- denotes )
T11649 1595-1596 . denotes .
T11650 1596-1817 sentence denotes The targeting construct was built and verified in pBSSK (Stratagene, La Jolla, California, United States), then digested with XhoI and subcloned into pSV1, the vector used for homologous recombination (Yang et al. 1997).
T11651 1597-1600 DT denotes The
T11653 1601-1610 NN denotes targeting
T11652 1611-1620 NN denotes construct
T11655 1621-1624 VBD denotes was
T11654 1625-1630 VBN denotes built
T11656 1631-1634 CC denotes and
T11657 1635-1643 VBN denotes verified
T11658 1644-1646 IN denotes in
T11659 1647-1652 NN denotes pBSSK
T11660 1653-1654 -LRB- denotes (
T11661 1654-1664 NNP denotes Stratagene
T11662 1664-1666 , denotes ,
T11663 1666-1668 NNP denotes La
T11664 1669-1674 NNP denotes Jolla
T11665 1674-1676 , denotes ,
T11666 1676-1686 NNP denotes California
T11667 1686-1688 , denotes ,
T11668 1688-1694 NNP denotes United
T11669 1695-1701 NNP denotes States
T11670 1701-1702 -RRB- denotes )
T11671 1702-1704 , denotes ,
T11672 1704-1708 RB denotes then
T11673 1709-1717 VBN denotes digested
T11674 1718-1722 IN denotes with
T11675 1723-1727 NN denotes XhoI
T11676 1728-1731 CC denotes and
T11677 1732-1741 VBN denotes subcloned
T11678 1742-1746 IN denotes into
T11679 1747-1751 NN denotes pSV1
T11680 1751-1753 , denotes ,
T11681 1753-1756 DT denotes the
T11682 1757-1763 NN denotes vector
T11683 1764-1768 VBN denotes used
T11684 1769-1772 IN denotes for
T11685 1773-1783 JJ denotes homologous
T11686 1784-1797 NN denotes recombination
T11687 1798-1799 -LRB- denotes (
T11688 1799-1803 NNP denotes Yang
T11689 1804-1806 FW denotes et
T11690 1807-1810 FW denotes al.
T11691 1811-1815 CD denotes 1997
T11692 1815-1816 -RRB- denotes )
T11693 1816-1817 . denotes .
T11694 1817-1965 sentence denotes Southern blotting, PCR, and DNA sequence analysis confirmed the appropriate targeting construct and BAC modifications were made (unpublished data).
T11695 1818-1826 NNP denotes Southern
T11696 1827-1835 NN denotes blotting
T11698 1835-1837 , denotes ,
T11699 1837-1840 NN denotes PCR
T11700 1840-1842 , denotes ,
T11701 1842-1845 CC denotes and
T11702 1846-1849 NN denotes DNA
T11703 1850-1858 NN denotes sequence
T11697 1859-1867 NN denotes analysis
T11704 1868-1877 VBD denotes confirmed
T11705 1878-1881 DT denotes the
T11707 1882-1893 JJ denotes appropriate
T11708 1894-1903 NN denotes targeting
T11706 1904-1913 NN denotes construct
T11710 1914-1917 CC denotes and
T11711 1918-1921 NN denotes BAC
T11712 1922-1935 NNS denotes modifications
T11713 1936-1940 VBD denotes were
T11709 1941-1945 VBN denotes made
T11714 1946-1947 -LRB- denotes (
T11716 1947-1958 JJ denotes unpublished
T11715 1959-1963 NNS denotes data
T11717 1963-1964 -RRB- denotes )
T11718 1964-1965 . denotes .
R7272 T11321 T11322 det A,library
R7273 T11322 T11328 nsubjpass library,screened
R7274 T11323 T11324 compound mouse,BAC
R7275 T11324 T11322 compound BAC,library
R7276 T11325 T11326 compound 129x1,SvJ
R7277 T11326 T11324 compound SvJ,BAC
R7278 T11327 T11326 punct /,SvJ
R7279 T11329 T11330 punct (,Invitrogen
R7280 T11330 T11322 parataxis Invitrogen,library
R7281 T11331 T11330 punct ),Invitrogen
R7282 T11332 T11328 auxpass was,screened
R7283 T11333 T11334 aux to,identify
R7284 T11334 T11328 advcl identify,screened
R7285 T11335 T11336 det a,BAC
R7286 T11336 T11334 dobj BAC,identify
R7287 T11337 T11338 nummod 140,kb
R7288 T11338 T11336 compound kb,BAC
R7289 T11339 T11338 punct -,kb
R7290 T11340 T11336 prep from,BAC
R7291 T11341 T11342 det the,locus
R7292 T11342 T11340 pobj locus,from
R7293 T11343 T11342 compound Gdf5,locus
R7294 T11344 T11328 punct .,screened
R7295 T11346 T11347 det This,BAC
R7296 T11347 T11348 nsubjpass BAC,modified
R7297 T11349 T11348 auxpass was,modified
R7298 T11350 T11348 advcl using,modified
R7299 T11351 T11352 det a,system
R7300 T11352 T11350 dobj system,using
R7301 T11353 T11354 amod homologous,recombination
R7302 T11354 T11352 compound recombination,system
R7303 T11355 T11350 prep in,using
R7304 T11356 T11357 compound E.,coli
R7305 T11357 T11355 pobj coli,in
R7306 T11358 T11359 punct (,Yang
R7307 T11359 T11350 meta Yang,using
R7308 T11360 T11359 nmod et,Yang
R7309 T11361 T11359 nmod al.,Yang
R7310 T11362 T11359 nummod 1997,Yang
R7311 T11363 T11359 punct ),Yang
R7312 T11364 T11365 aux to,place
R7313 T11365 T11350 advcl place,using
R7314 T11366 T11367 amod nuclear,localized
R7315 T11367 T11369 amod localized,recombinase
R7316 T11368 T11367 punct -,localized
R7317 T11369 T11365 dobj recombinase,place
R7318 T11370 T11369 compound Cre,recombinase
R7319 T11371 T11369 punct (,recombinase
R7320 T11372 T11369 prep from,recombinase
R7321 T11373 T11374 compound plasmid,pML78
R7322 T11374 T11372 pobj pML78,from
R7323 T11375 T11376 punct ", ",gift
R7324 T11376 T11374 parataxis gift,pML78
R7325 T11377 T11376 prep of,gift
R7326 T11378 T11379 compound Gail,Martin
R7327 T11379 T11377 pobj Martin,of
R7328 T11380 T11369 punct ),recombinase
R7329 T11381 T11369 acl followed,recombinase
R7330 T11382 T11381 agent by,followed
R7331 T11383 T11384 compound IRES,hPLAP
R7332 T11384 T11382 pobj hPLAP,by
R7333 T11385 T11384 punct -,hPLAP
R7334 T11386 T11384 punct (,hPLAP
R7335 T11387 T11384 prep from,hPLAP
R7336 T11388 T11387 pobj plasmid,from
R7337 T11389 T11388 nummod 1726,plasmid
R7338 T11390 T11388 punct ", ",plasmid
R7339 T11391 T11388 parataxis gift,plasmid
R7340 T11392 T11391 prep of,gift
R7341 T11393 T11394 compound Oliver,Bogler
R7342 T11394 T11392 pobj Bogler,of
R7343 T11395 T11384 punct ),hPLAP
R7344 T11396 T11397 advmod directly,behind
R7345 T11397 T11384 prep behind,hPLAP
R7346 T11398 T11399 det the,site
R7347 T11399 T11397 pobj site,behind
R7348 T11400 T11399 compound ATG,site
R7349 T11401 T11399 compound start,site
R7350 T11402 T11399 prep of,site
R7351 T11403 T11402 pobj Gdf5,of
R7352 T11404 T11348 punct .,modified
R7353 T11406 T11407 prep In,removed
R7354 T11408 T11409 det the,process
R7355 T11409 T11406 pobj process,In
R7356 T11410 T11407 punct ", ",removed
R7357 T11411 T11412 nummod 583,bp
R7358 T11412 T11407 nsubjpass bp,removed
R7359 T11413 T11412 prep of,bp
R7360 T11414 T11415 det the,exon
R7361 T11415 T11413 pobj exon,of
R7362 T11416 T11415 amod first,exon
R7363 T11417 T11415 prep of,exon
R7364 T11418 T11417 pobj Gdf5,of
R7365 T11419 T11407 auxpass was,removed
R7366 T11420 T11407 cc and,removed
R7367 T11421 T11422 det no,protein
R7368 T11422 T11425 nsubjpass protein,predicted
R7369 T11423 T11422 amod functional,protein
R7370 T11424 T11422 compound GDF5,protein
R7371 T11425 T11407 conj predicted,removed
R7372 T11426 T11425 auxpass is,predicted
R7373 T11427 T11428 aux to,produced
R7374 T11428 T11425 xcomp produced,predicted
R7375 T11429 T11428 auxpass be,produced
R7376 T11430 T11425 punct .,predicted
R7377 T11432 T11433 det The,arm
R7378 T11433 T11437 nsubjpass arm,subcloned
R7379 T11434 T11433 nummod 5,arm
R7380 T11435 T11434 punct ′,5
R7381 T11436 T11433 compound homology,arm
R7382 T11438 T11437 auxpass was,subcloned
R7383 T11439 T11437 prep from,subcloned
R7384 T11440 T11441 det a,product
R7385 T11441 T11439 pobj product,from
R7386 T11442 T11441 compound PCR,product
R7387 T11443 T11441 acl tailed,product
R7388 T11444 T11443 prep with,tailed
R7389 T11445 T11446 nmod XhoI,sites
R7390 T11446 T11444 pobj sites,with
R7391 T11447 T11445 cc and,XhoI
R7392 T11448 T11445 conj Bsp120I,XhoI
R7393 T11449 T11446 compound restriction,sites
R7394 T11450 T11451 dep that,contains
R7395 T11451 T11441 relcl contains,product
R7396 T11452 T11453 nummod 781,bp
R7397 T11453 T11451 dobj bp,contains
R7398 T11454 T11453 prep of,bp
R7399 T11455 T11456 nummod 5,sequence
R7400 T11456 T11454 pobj sequence,of
R7401 T11457 T11455 punct ′,5
R7402 T11458 T11456 amod genomic,sequence
R7403 T11459 T11456 compound Gdf5,sequence
R7404 T11460 T11456 acl ending,sequence
R7405 T11461 T11460 prep at,ending
R7406 T11462 T11463 det the,site
R7407 T11463 T11461 pobj site,at
R7408 T11464 T11465 compound ATG,translation
R7409 T11465 T11463 compound translation,site
R7410 T11466 T11463 compound start,site
R7411 T11467 T11468 punct (,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7412 T11468 T11460 parataxis GTTTGGGCCCATCCTCTGGCCAGCCGCTG,ending
R7413 T11469 T11470 amod forward,primer
R7414 T11470 T11471 dep primer,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7415 T11471 T11468 dep CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7416 T11472 T11471 nummod 5,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7417 T11473 T11472 punct ′,5
R7418 T11474 T11471 punct -,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7419 T11475 T11471 punct -,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7420 T11476 T11471 nummod 3,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7421 T11477 T11471 punct ′,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7422 T11478 T11468 punct ;,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7423 T11479 T11468 amod reverse,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7424 T11480 T11468 nummod 5,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7425 T11481 T11480 punct ′,5
R7426 T11482 T11468 punct -,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7427 T11483 T11468 punct -,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7428 T11484 T11468 nummod 3,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7429 T11485 T11468 punct ′,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7430 T11486 T11468 punct ),GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7431 T11487 T11437 punct .,subcloned
R7432 T11489 T11490 nsubjpass Cre,subcloned
R7433 T11491 T11490 auxpass was,subcloned
R7434 T11492 T11490 prep from,subcloned
R7435 T11493 T11494 det a,fragment
R7436 T11494 T11492 pobj fragment,from
R7437 T11495 T11496 nummod 1.1,kb
R7438 T11496 T11494 compound kb,fragment
R7439 T11497 T11496 punct -,kb
R7440 T11498 T11499 compound Bsp120I,EcoRI
R7441 T11499 T11494 compound EcoRI,fragment
R7442 T11500 T11499 punct /,EcoRI
R7443 T11501 T11494 prep of,fragment
R7444 T11502 T11501 pobj pML78,of
R7445 T11503 T11490 punct .,subcloned
R7446 T11505 T11506 compound IRES,hPLAP
R7447 T11506 T11507 nsubjpass hPLAP,subcloned
R7448 T11508 T11507 auxpass was,subcloned
R7449 T11509 T11507 prep from,subcloned
R7450 T11510 T11511 det a,product
R7451 T11511 T11509 pobj product,from
R7452 T11512 T11513 nummod 2.1,kb
R7453 T11513 T11511 compound kb,product
R7454 T11514 T11513 punct -,kb
R7455 T11515 T11511 compound PCR,product
R7456 T11516 T11511 acl tailed,product
R7457 T11517 T11516 prep with,tailed
R7458 T11518 T11519 nmod EcoRI,sites
R7459 T11519 T11517 pobj sites,with
R7460 T11520 T11518 cc and,EcoRI
R7461 T11521 T11518 conj SpeI,EcoRI
R7462 T11522 T11523 dep that,contains
R7463 T11523 T11511 relcl contains,product
R7464 T11524 T11525 det the,site
R7465 T11525 T11523 dobj site,contains
R7466 T11526 T11527 compound hPLAP,translation
R7467 T11527 T11525 compound translation,site
R7468 T11528 T11525 compound stop,site
R7469 T11529 T11530 punct (,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7470 T11530 T11525 parataxis AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG,site
R7471 T11531 T11532 amod forward,primer
R7472 T11532 T11533 dep primer,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7473 T11533 T11530 dep ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7474 T11534 T11533 nummod 5,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7475 T11535 T11534 punct ′,5
R7476 T11536 T11533 punct -,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7477 T11537 T11533 punct -,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7478 T11538 T11533 nummod 3,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7479 T11539 T11533 punct ′,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7480 T11540 T11530 punct ;,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7481 T11541 T11530 amod reverse,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7482 T11542 T11530 nummod 5,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7483 T11543 T11542 punct ′,5
R7484 T11544 T11530 punct -,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7485 T11545 T11530 punct -,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7486 T11546 T11530 nummod 3,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7487 T11547 T11530 punct ′,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7488 T11548 T11530 punct ),AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7489 T11549 T11507 punct .,subcloned
R7490 T11551 T11552 det The,arm
R7491 T11552 T11556 nsubjpass arm,subcloned
R7492 T11553 T11552 nummod 3,arm
R7493 T11554 T11553 punct ′,3
R7494 T11555 T11552 compound homology,arm
R7495 T11557 T11556 auxpass was,subcloned
R7496 T11558 T11556 prep from,subcloned
R7497 T11559 T11560 det a,product
R7498 T11560 T11558 pobj product,from
R7499 T11561 T11562 nummod 0.8,kb
R7500 T11562 T11560 compound kb,product
R7501 T11563 T11562 punct -,kb
R7502 T11564 T11560 compound PCR,product
R7503 T11565 T11560 acl amplified,product
R7504 T11566 T11565 prep from,amplified
R7505 T11567 T11568 det a,subclone
R7506 T11568 T11566 pobj subclone,from
R7507 T11569 T11570 nummod 0.9,kb
R7508 T11570 T11568 nmod kb,subclone
R7509 T11571 T11570 punct -,kb
R7510 T11572 T11573 nmod XhoI,Gdf5
R7511 T11573 T11568 nmod Gdf5,subclone
R7512 T11574 T11568 amod genomic,subclone
R7513 T11575 T11568 acl containing,subclone
R7514 T11576 T11575 dobj part,containing
R7515 T11577 T11576 prep of,part
R7516 T11578 T11579 det the,exon
R7517 T11579 T11577 pobj exon,of
R7518 T11580 T11579 amod first,exon
R7519 T11581 T11579 cc and,exon
R7520 T11582 T11583 amod downstream,intron
R7521 T11583 T11579 conj intron,exon
R7522 T11584 T11556 punct .,subcloned
R7523 T11586 T11587 det The,primer
R7524 T11587 T11589 nsubj primer,contains
R7525 T11588 T11587 amod forward,primer
R7526 T11589 T11590 ccomp contains,is
R7527 T11591 T11592 det the,end
R7528 T11592 T11589 dobj end,contains
R7529 T11593 T11592 nummod 3,end
R7530 T11594 T11593 punct ′,3
R7531 T11595 T11592 prep of,end
R7532 T11596 T11597 det the,exon
R7533 T11597 T11595 pobj exon,of
R7534 T11598 T11597 amod first,exon
R7535 T11599 T11589 cc and,contains
R7536 T11600 T11601 auxpass is,tailed
R7537 T11601 T11589 conj tailed,contains
R7538 T11602 T11601 prep with,tailed
R7539 T11603 T11604 det a,site
R7540 T11604 T11602 pobj site,with
R7541 T11605 T11604 compound SpeI,site
R7542 T11606 T11590 punct ;,is
R7543 T11607 T11608 det the,primer
R7544 T11608 T11590 nsubj primer,is
R7545 T11609 T11608 amod reverse,primer
R7546 T11610 T11590 prep from,is
R7547 T11611 T11612 det the,promoter
R7548 T11612 T11610 pobj promoter,from
R7549 T11613 T11612 compound T7,promoter
R7550 T11614 T11612 prep of,promoter
R7551 T11615 T11616 det the,vector
R7552 T11616 T11614 pobj vector,of
R7553 T11617 T11616 acl containing,vector
R7554 T11618 T11619 det the,subclone
R7555 T11619 T11617 dobj subclone,containing
R7556 T11620 T11621 nummod 0.9,kb
R7557 T11621 T11619 compound kb,subclone
R7558 T11622 T11621 punct -,kb
R7559 T11623 T11590 cc and,is
R7560 T11624 T11590 conj flanks,is
R7561 T11625 T11626 det the,site
R7562 T11626 T11624 dobj site,flanks
R7563 T11627 T11628 amod intronic,XhoI
R7564 T11628 T11626 compound XhoI,site
R7565 T11629 T11630 punct (,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7566 T11630 T11626 parataxis GATTTCTAGAGTAATACGACTCACTATAGGGC,site
R7567 T11631 T11632 amod forward,primer
R7568 T11632 T11633 dep primer,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7569 T11633 T11630 dep CTAAACTAGTCACCAGCTTTATTGACAAAGG,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7570 T11634 T11633 nummod 5,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7571 T11635 T11634 punct ′,5
R7572 T11636 T11633 punct -,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7573 T11637 T11633 punct -,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7574 T11638 T11633 nummod 3,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7575 T11639 T11633 punct ′,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7576 T11640 T11630 punct ;,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7577 T11641 T11630 amod reverse,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7578 T11642 T11630 nummod 5,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7579 T11643 T11642 punct ′,5
R7580 T11644 T11630 punct -,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7581 T11645 T11630 punct -,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7582 T11646 T11630 nummod 3,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7583 T11647 T11630 punct ′,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7584 T11648 T11630 punct ),GATTTCTAGAGTAATACGACTCACTATAGGGC
R7585 T11649 T11590 punct .,is
R7586 T11651 T11652 det The,construct
R7587 T11652 T11654 nsubjpass construct,built
R7588 T11653 T11652 compound targeting,construct
R7589 T11655 T11654 auxpass was,built
R7590 T11656 T11654 cc and,built
R7591 T11657 T11654 conj verified,built
R7592 T11658 T11657 prep in,verified
R7593 T11659 T11658 pobj pBSSK,in
R7594 T11660 T11661 punct (,Stratagene
R7595 T11661 T11659 parataxis Stratagene,pBSSK
R7596 T11662 T11661 punct ", ",Stratagene
R7597 T11663 T11664 compound La,Jolla
R7598 T11664 T11661 npadvmod Jolla,Stratagene
R7599 T11665 T11661 punct ", ",Stratagene
R7600 T11666 T11661 npadvmod California,Stratagene
R7601 T11667 T11661 punct ", ",Stratagene
R7602 T11668 T11669 compound United,States
R7603 T11669 T11661 npadvmod States,Stratagene
R7604 T11670 T11661 punct ),Stratagene
R7605 T11671 T11654 punct ", ",built
R7606 T11672 T11673 advmod then,digested
R7607 T11673 T11654 conj digested,built
R7608 T11674 T11673 prep with,digested
R7609 T11675 T11674 pobj XhoI,with
R7610 T11676 T11673 cc and,digested
R7611 T11677 T11673 conj subcloned,digested
R7612 T11678 T11677 prep into,subcloned
R7613 T11679 T11678 pobj pSV1,into
R7614 T11680 T11679 punct ", ",pSV1
R7615 T11681 T11682 det the,vector
R7616 T11682 T11679 appos vector,pSV1
R7617 T11683 T11682 acl used,vector
R7618 T11684 T11683 prep for,used
R7619 T11685 T11686 amod homologous,recombination
R7620 T11686 T11684 pobj recombination,for
R7621 T11687 T11688 punct (,Yang
R7622 T11688 T11683 meta Yang,used
R7623 T11689 T11688 nmod et,Yang
R7624 T11690 T11688 nmod al.,Yang
R7625 T11691 T11688 nummod 1997,Yang
R7626 T11692 T11688 punct ),Yang
R7627 T11693 T11654 punct .,built
R7628 T11695 T11696 nmod Southern,blotting
R7629 T11696 T11697 nmod blotting,analysis
R7630 T11697 T11704 nsubj analysis,confirmed
R7631 T11698 T11696 punct ", ",blotting
R7632 T11699 T11696 conj PCR,blotting
R7633 T11700 T11699 punct ", ",PCR
R7634 T11701 T11699 cc and,PCR
R7635 T11702 T11703 compound DNA,sequence
R7636 T11703 T11699 conj sequence,PCR
R7637 T11705 T11706 det the,construct
R7638 T11706 T11709 nsubjpass construct,made
R7639 T11707 T11706 amod appropriate,construct
R7640 T11708 T11706 compound targeting,construct
R7641 T11709 T11704 advcl made,confirmed
R7642 T11710 T11706 cc and,construct
R7643 T11711 T11712 compound BAC,modifications
R7644 T11712 T11706 conj modifications,construct
R7645 T11713 T11709 auxpass were,made
R7646 T11714 T11715 punct (,data
R7647 T11715 T11709 meta data,made
R7648 T11716 T11715 amod unpublished,data
R7649 T11717 T11715 punct ),data
R7650 T11718 T11704 punct .,confirmed