PMC:523229 / 50516-60660 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T11313 23-33 NN denotes Generation
T11314 34-36 IN denotes of
T11315 37-41 NN denotes Gdf5
T11317 41-42 HYPH denotes -
T11316 42-45 NN denotes Cre
T11318 46-56 JJ denotes transgenic
T11319 57-61 NNS denotes mice
T11320 61-163 sentence denotes A mouse 129x1/SvJ BAC library (Invitrogen) was screened to identify a 140-kb BAC from the Gdf5 locus.
T11321 62-63 DT denotes A
T11323 64-69 NN denotes mouse
T11325 70-75 NN denotes 129x1
T11327 75-76 HYPH denotes /
T11326 76-79 NN denotes SvJ
T11324 80-83 NN denotes BAC
T11322 84-91 NN denotes library
T11329 92-93 -LRB- denotes (
T11330 93-103 NNP denotes Invitrogen
T11331 103-104 -RRB- denotes )
T11332 105-108 VBD denotes was
T11328 109-117 VBN denotes screened
T11333 118-120 TO denotes to
T11334 121-129 VB denotes identify
T11335 130-131 DT denotes a
T11337 132-135 CD denotes 140
T11339 135-136 HYPH denotes -
T11338 136-138 NN denotes kb
T11336 139-142 NN denotes BAC
T11340 143-147 IN denotes from
T11341 148-151 DT denotes the
T11343 152-156 NN denotes Gdf5
T11342 157-162 NN denotes locus
T11344 162-163 . denotes .
T11345 163-450 sentence denotes This BAC was modified using a homologous recombination system in E. coli (Yang et al. 1997) to place nuclear-localized Cre recombinase (from plasmid pML78, gift of Gail Martin) followed by IRES-hPLAP (from plasmid 1726, gift of Oliver Bogler) directly behind the ATG start site of Gdf5.
T11346 164-168 DT denotes This
T11347 169-172 NN denotes BAC
T11349 173-176 VBD denotes was
T11348 177-185 VBN denotes modified
T11350 186-191 VBG denotes using
T11351 192-193 DT denotes a
T11353 194-204 JJ denotes homologous
T11354 205-218 NN denotes recombination
T11352 219-225 NN denotes system
T11355 226-228 IN denotes in
T11356 229-231 NNP denotes E.
T11357 232-236 NNP denotes coli
T11358 237-238 -LRB- denotes (
T11359 238-242 NNP denotes Yang
T11360 243-245 FW denotes et
T11361 246-249 FW denotes al.
T11362 250-254 CD denotes 1997
T11363 254-255 -RRB- denotes )
T11364 256-258 TO denotes to
T11365 259-264 VB denotes place
T11366 265-272 JJ denotes nuclear
T11368 272-273 HYPH denotes -
T11367 273-282 VBN denotes localized
T11370 283-286 NN denotes Cre
T11369 287-298 NN denotes recombinase
T11371 299-300 -LRB- denotes (
T11372 300-304 IN denotes from
T11373 305-312 NN denotes plasmid
T11374 313-318 NN denotes pML78
T11375 318-320 , denotes ,
T11376 320-324 NN denotes gift
T11377 325-327 IN denotes of
T11378 328-332 NNP denotes Gail
T11379 333-339 NNP denotes Martin
T11380 339-340 -RRB- denotes )
T11381 341-349 VBN denotes followed
T11382 350-352 IN denotes by
T11383 353-357 NN denotes IRES
T11385 357-358 HYPH denotes -
T11384 358-363 NN denotes hPLAP
T11386 364-365 -LRB- denotes (
T11387 365-369 IN denotes from
T11388 370-377 NN denotes plasmid
T11389 378-382 CD denotes 1726
T11390 382-384 , denotes ,
T11391 384-388 NN denotes gift
T11392 389-391 IN denotes of
T11393 392-398 NNP denotes Oliver
T11394 399-405 NNP denotes Bogler
T11395 405-406 -RRB- denotes )
T11396 407-415 RB denotes directly
T11397 416-422 IN denotes behind
T11398 423-426 DT denotes the
T11400 427-430 NN denotes ATG
T11401 431-436 NN denotes start
T11399 437-441 NN denotes site
T11402 442-444 IN denotes of
T11403 445-449 NN denotes Gdf5
T11404 449-450 . denotes .
T11405 450-571 sentence denotes In the process, 583 bp of the first exon of Gdf5 was removed and no functional GDF5 protein is predicted to be produced.
T11406 451-453 IN denotes In
T11408 454-457 DT denotes the
T11409 458-465 NN denotes process
T11410 465-467 , denotes ,
T11411 467-470 CD denotes 583
T11412 471-473 NNS denotes bp
T11413 474-476 IN denotes of
T11414 477-480 DT denotes the
T11416 481-486 JJ denotes first
T11415 487-491 NN denotes exon
T11417 492-494 IN denotes of
T11418 495-499 NN denotes Gdf5
T11419 500-503 VBD denotes was
T11407 504-511 VBN denotes removed
T11420 512-515 CC denotes and
T11421 516-518 DT denotes no
T11423 519-529 JJ denotes functional
T11424 530-534 NN denotes GDF5
T11422 535-542 NN denotes protein
T11426 543-545 VBZ denotes is
T11425 546-555 VBN denotes predicted
T11427 556-558 TO denotes to
T11429 559-561 VB denotes be
T11428 562-570 VBN denotes produced
T11430 570-571 . denotes .
T11431 571-863 sentence denotes The 5′ homology arm was subcloned from a PCR product tailed with XhoI and Bsp120I restriction sites that contains 781 bp of 5′ genomic Gdf5 sequence ending at the ATG translation start site (forward primer 5′-CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC-3′; reverse 5′-GTTTGGGCCCATCCTCTGGCCAGCCGCTG-3′).
T11432 572-575 DT denotes The
T11434 576-577 CD denotes 5
T11435 577-578 SYM denotes
T11436 579-587 NN denotes homology
T11433 588-591 NN denotes arm
T11438 592-595 VBD denotes was
T11437 596-605 VBN denotes subcloned
T11439 606-610 IN denotes from
T11440 611-612 DT denotes a
T11442 613-616 NN denotes PCR
T11441 617-624 NN denotes product
T11443 625-631 VBN denotes tailed
T11444 632-636 IN denotes with
T11445 637-641 NN denotes XhoI
T11447 642-645 CC denotes and
T11448 646-653 NN denotes Bsp120I
T11449 654-665 NN denotes restriction
T11446 666-671 NNS denotes sites
T11450 672-676 WDT denotes that
T11451 677-685 VBZ denotes contains
T11452 686-689 CD denotes 781
T11453 690-692 NNS denotes bp
T11454 693-695 IN denotes of
T11455 696-697 CD denotes 5
T11457 697-698 SYM denotes
T11458 699-706 JJ denotes genomic
T11459 707-711 NN denotes Gdf5
T11456 712-720 NN denotes sequence
T11460 721-727 VBG denotes ending
T11461 728-730 IN denotes at
T11462 731-734 DT denotes the
T11464 735-738 NN denotes ATG
T11465 739-750 NN denotes translation
T11466 751-756 NN denotes start
T11463 757-761 NN denotes site
T11467 762-763 -LRB- denotes (
T11469 763-770 JJ denotes forward
T11470 771-777 NN denotes primer
T11472 778-779 CD denotes 5
T11473 779-780 SYM denotes
T11474 780-781 HYPH denotes -
T11471 781-813 NN denotes CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
T11475 813-814 HYPH denotes -
T11476 814-815 CD denotes 3
T11477 815-816 SYM denotes
T11478 816-817 : denotes ;
T11479 818-825 JJ denotes reverse
T11480 826-827 CD denotes 5
T11481 827-828 SYM denotes
T11482 828-829 HYPH denotes -
T11468 829-858 NN denotes GTTTGGGCCCATCCTCTGGCCAGCCGCTG
T11483 858-859 HYPH denotes -
T11484 859-860 CD denotes 3
T11485 860-861 SYM denotes
T11486 861-862 -RRB- denotes )
T11487 862-863 . denotes .
T11488 863-928 sentence denotes Cre was subcloned from a 1.1-kb Bsp120I/EcoRI fragment of pML78.
T11489 864-867 NN denotes Cre
T11491 868-871 VBD denotes was
T11490 872-881 VBN denotes subcloned
T11492 882-886 IN denotes from
T11493 887-888 DT denotes a
T11495 889-892 CD denotes 1.1
T11497 892-893 HYPH denotes -
T11496 893-895 NN denotes kb
T11498 896-903 NN denotes Bsp120I
T11500 903-904 HYPH denotes /
T11499 904-909 NN denotes EcoRI
T11494 910-918 NN denotes fragment
T11501 919-921 IN denotes of
T11502 922-927 NN denotes pML78
T11503 927-928 . denotes .
T11504 928-1175 sentence denotes IRES hPLAP was subcloned from a 2.1-kb PCR product tailed with EcoRI and SpeI sites that contains the hPLAP translation stop site (forward primer 5′-ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG-3′; reverse 5′-AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG-3′).
T11505 929-933 NN denotes IRES
T11506 934-939 NN denotes hPLAP
T11508 940-943 VBD denotes was
T11507 944-953 VBN denotes subcloned
T11509 954-958 IN denotes from
T11510 959-960 DT denotes a
T11512 961-964 CD denotes 2.1
T11514 964-965 HYPH denotes -
T11513 965-967 NN denotes kb
T11515 968-971 NN denotes PCR
T11511 972-979 NN denotes product
T11516 980-986 VBN denotes tailed
T11517 987-991 IN denotes with
T11518 992-997 NN denotes EcoRI
T11520 998-1001 CC denotes and
T11521 1002-1006 NN denotes SpeI
T11519 1007-1012 NNS denotes sites
T11522 1013-1017 WDT denotes that
T11523 1018-1026 VBZ denotes contains
T11524 1027-1030 DT denotes the
T11526 1031-1036 NN denotes hPLAP
T11527 1037-1048 NN denotes translation
T11528 1049-1053 NN denotes stop
T11525 1054-1058 NN denotes site
T11529 1059-1060 -LRB- denotes (
T11531 1060-1067 JJ denotes forward
T11532 1068-1074 NN denotes primer
T11534 1075-1076 CD denotes 5
T11535 1076-1077 SYM denotes
T11536 1077-1078 HYPH denotes -
T11533 1078-1116 NN denotes ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
T11537 1116-1117 HYPH denotes -
T11538 1117-1118 CD denotes 3
T11539 1118-1119 SYM denotes
T11540 1119-1120 : denotes ;
T11541 1121-1128 JJ denotes reverse
T11542 1129-1130 CD denotes 5
T11543 1130-1131 SYM denotes
T11544 1131-1132 HYPH denotes -
T11530 1132-1170 NN denotes AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
T11545 1170-1171 HYPH denotes -
T11546 1171-1172 CD denotes 3
T11547 1172-1173 SYM denotes
T11548 1173-1174 -RRB- denotes )
T11549 1174-1175 . denotes .
T11550 1175-1343 sentence denotes The 3′ homology arm was subcloned from a 0.8-kb PCR product amplified from a 0.9-kb XhoI Gdf5 genomic subclone containing part of the first exon and downstream intron.
T11551 1176-1179 DT denotes The
T11553 1180-1181 CD denotes 3
T11554 1181-1182 SYM denotes
T11555 1183-1191 NN denotes homology
T11552 1192-1195 NN denotes arm
T11557 1196-1199 VBD denotes was
T11556 1200-1209 VBN denotes subcloned
T11558 1210-1214 IN denotes from
T11559 1215-1216 DT denotes a
T11561 1217-1220 CD denotes 0.8
T11563 1220-1221 HYPH denotes -
T11562 1221-1223 NN denotes kb
T11564 1224-1227 NN denotes PCR
T11560 1228-1235 NN denotes product
T11565 1236-1245 VBN denotes amplified
T11566 1246-1250 IN denotes from
T11567 1251-1252 DT denotes a
T11569 1253-1256 CD denotes 0.9
T11571 1256-1257 HYPH denotes -
T11570 1257-1259 NN denotes kb
T11572 1260-1264 NN denotes XhoI
T11573 1265-1269 NN denotes Gdf5
T11574 1270-1277 JJ denotes genomic
T11568 1278-1286 NN denotes subclone
T11575 1287-1297 VBG denotes containing
T11576 1298-1302 NN denotes part
T11577 1303-1305 IN denotes of
T11578 1306-1309 DT denotes the
T11580 1310-1315 JJ denotes first
T11579 1316-1320 NN denotes exon
T11581 1321-1324 CC denotes and
T11582 1325-1335 JJ denotes downstream
T11583 1336-1342 NN denotes intron
T11584 1342-1343 . denotes .
T11585 1343-1658 sentence denotes The forward primer contains the 3′ end of the first exon and is tailed with a SpeI site; the reverse primer is from the T7 promoter of the vector containing the 0.9-kb subclone and flanks the intronic XhoI site (forward primer 5′-CTAAACTAGTCACCAGCTTTATTGACAAAGG-3′; reverse 5′-GATTTCTAGAGTAATACGACTCACTATAGGGC-3′).
T11586 1344-1347 DT denotes The
T11588 1348-1355 JJ denotes forward
T11587 1356-1362 NN denotes primer
T11589 1363-1371 VBZ denotes contains
T11591 1372-1375 DT denotes the
T11593 1376-1377 CD denotes 3
T11594 1377-1378 SYM denotes
T11592 1379-1382 NN denotes end
T11595 1383-1385 IN denotes of
T11596 1386-1389 DT denotes the
T11598 1390-1395 JJ denotes first
T11597 1396-1400 NN denotes exon
T11599 1401-1404 CC denotes and
T11600 1405-1407 VBZ denotes is
T11601 1408-1414 VBN denotes tailed
T11602 1415-1419 IN denotes with
T11603 1420-1421 DT denotes a
T11605 1422-1426 NN denotes SpeI
T11604 1427-1431 NN denotes site
T11606 1431-1432 : denotes ;
T11607 1433-1436 DT denotes the
T11609 1437-1444 JJ denotes reverse
T11608 1445-1451 NN denotes primer
T11590 1452-1454 VBZ denotes is
T11610 1455-1459 IN denotes from
T11611 1460-1463 DT denotes the
T11613 1464-1466 NN denotes T7
T11612 1467-1475 NN denotes promoter
T11614 1476-1478 IN denotes of
T11615 1479-1482 DT denotes the
T11616 1483-1489 NN denotes vector
T11617 1490-1500 VBG denotes containing
T11618 1501-1504 DT denotes the
T11620 1505-1508 CD denotes 0.9
T11622 1508-1509 HYPH denotes -
T11621 1509-1511 NN denotes kb
T11619 1512-1520 NN denotes subclone
T11623 1521-1524 CC denotes and
T11624 1525-1531 VBZ denotes flanks
T11625 1532-1535 DT denotes the
T11627 1536-1544 JJ denotes intronic
T11628 1545-1549 NN denotes XhoI
T11626 1550-1554 NN denotes site
T11629 1555-1556 -LRB- denotes (
T11631 1556-1563 JJ denotes forward
T11632 1564-1570 NN denotes primer
T11634 1571-1572 CD denotes 5
T11635 1572-1573 SYM denotes
T11636 1573-1574 HYPH denotes -
T11633 1574-1605 NN denotes CTAAACTAGTCACCAGCTTTATTGACAAAGG
T11637 1605-1606 HYPH denotes -
T11638 1606-1607 CD denotes 3
T11639 1607-1608 SYM denotes
T11640 1608-1609 : denotes ;
T11641 1610-1617 JJ denotes reverse
T11642 1618-1619 CD denotes 5
T11643 1619-1620 SYM denotes
T11644 1620-1621 HYPH denotes -
T11630 1621-1653 NN denotes GATTTCTAGAGTAATACGACTCACTATAGGGC
T11645 1653-1654 HYPH denotes -
T11646 1654-1655 CD denotes 3
T11647 1655-1656 SYM denotes
T11648 1656-1657 -RRB- denotes )
T11649 1657-1658 . denotes .
T11650 1658-1879 sentence denotes The targeting construct was built and verified in pBSSK (Stratagene, La Jolla, California, United States), then digested with XhoI and subcloned into pSV1, the vector used for homologous recombination (Yang et al. 1997).
T11651 1659-1662 DT denotes The
T11653 1663-1672 NN denotes targeting
T11652 1673-1682 NN denotes construct
T11655 1683-1686 VBD denotes was
T11654 1687-1692 VBN denotes built
T11656 1693-1696 CC denotes and
T11657 1697-1705 VBN denotes verified
T11658 1706-1708 IN denotes in
T11659 1709-1714 NN denotes pBSSK
T11660 1715-1716 -LRB- denotes (
T11661 1716-1726 NNP denotes Stratagene
T11662 1726-1728 , denotes ,
T11663 1728-1730 NNP denotes La
T11664 1731-1736 NNP denotes Jolla
T11665 1736-1738 , denotes ,
T11666 1738-1748 NNP denotes California
T11667 1748-1750 , denotes ,
T11668 1750-1756 NNP denotes United
T11669 1757-1763 NNP denotes States
T11670 1763-1764 -RRB- denotes )
T11671 1764-1766 , denotes ,
T11672 1766-1770 RB denotes then
T11673 1771-1779 VBN denotes digested
T11674 1780-1784 IN denotes with
T11675 1785-1789 NN denotes XhoI
T11676 1790-1793 CC denotes and
T11677 1794-1803 VBN denotes subcloned
T11678 1804-1808 IN denotes into
T11679 1809-1813 NN denotes pSV1
T11680 1813-1815 , denotes ,
T11681 1815-1818 DT denotes the
T11682 1819-1825 NN denotes vector
T11683 1826-1830 VBN denotes used
T11684 1831-1834 IN denotes for
T11685 1835-1845 JJ denotes homologous
T11686 1846-1859 NN denotes recombination
T11687 1860-1861 -LRB- denotes (
T11688 1861-1865 NNP denotes Yang
T11689 1866-1868 FW denotes et
T11690 1869-1872 FW denotes al.
T11691 1873-1877 CD denotes 1997
T11692 1877-1878 -RRB- denotes )
T11693 1878-1879 . denotes .
T11694 1879-2027 sentence denotes Southern blotting, PCR, and DNA sequence analysis confirmed the appropriate targeting construct and BAC modifications were made (unpublished data).
T11695 1880-1888 NNP denotes Southern
T11696 1889-1897 NN denotes blotting
T11698 1897-1899 , denotes ,
T11699 1899-1902 NN denotes PCR
T11700 1902-1904 , denotes ,
T11701 1904-1907 CC denotes and
T11702 1908-1911 NN denotes DNA
T11703 1912-1920 NN denotes sequence
T11697 1921-1929 NN denotes analysis
T11704 1930-1939 VBD denotes confirmed
T11705 1940-1943 DT denotes the
T11707 1944-1955 JJ denotes appropriate
T11708 1956-1965 NN denotes targeting
T11706 1966-1975 NN denotes construct
T11710 1976-1979 CC denotes and
T11711 1980-1983 NN denotes BAC
T11712 1984-1997 NNS denotes modifications
T11713 1998-2002 VBD denotes were
T11709 2003-2007 VBN denotes made
T11714 2008-2009 -LRB- denotes (
T11716 2009-2020 JJ denotes unpublished
T11715 2021-2025 NNS denotes data
T11717 2025-2026 -RRB- denotes )
T11718 2026-2027 . denotes .
T11719 2027-2229 sentence denotes Before the modified BAC was injected to produce transgenic animals, a loxP site present in the BAC vector, pBeloBAC11, was removed to prevent the addition of undesired Cre target sites into the genome.
T11720 2028-2034 IN denotes Before
T11722 2035-2038 DT denotes the
T11724 2039-2047 VBN denotes modified
T11723 2048-2051 NN denotes BAC
T11725 2052-2055 VBD denotes was
T11721 2056-2064 VBN denotes injected
T11727 2065-2067 TO denotes to
T11728 2068-2075 VB denotes produce
T11729 2076-2086 JJ denotes transgenic
T11730 2087-2094 NNS denotes animals
T11731 2094-2096 , denotes ,
T11732 2096-2097 DT denotes a
T11734 2098-2102 NN denotes loxP
T11733 2103-2107 NN denotes site
T11735 2108-2115 JJ denotes present
T11736 2116-2118 IN denotes in
T11737 2119-2122 DT denotes the
T11739 2123-2126 NN denotes BAC
T11738 2127-2133 NN denotes vector
T11740 2133-2135 , denotes ,
T11741 2135-2145 NN denotes pBeloBAC11
T11742 2145-2147 , denotes ,
T11743 2147-2150 VBD denotes was
T11726 2151-2158 VBN denotes removed
T11744 2159-2161 TO denotes to
T11745 2162-2169 VB denotes prevent
T11746 2170-2173 DT denotes the
T11747 2174-2182 NN denotes addition
T11748 2183-2185 IN denotes of
T11749 2186-2195 JJ denotes undesired
T11751 2196-2199 NN denotes Cre
T11752 2200-2206 NN denotes target
T11750 2207-2212 NNS denotes sites
T11753 2213-2217 IN denotes into
T11754 2218-2221 DT denotes the
T11755 2222-2228 NN denotes genome
T11756 2228-2229 . denotes .
T11757 2229-2384 sentence denotes To do this, BAC DNA was prepared by CsCl separation, digested with NotI to free the insert from the vector, and size-fractionated over a sucrose gradient.
T11758 2230-2232 TO denotes To
T11759 2233-2235 VB denotes do
T11761 2236-2240 DT denotes this
T11762 2240-2242 , denotes ,
T11763 2242-2245 NN denotes BAC
T11764 2246-2249 NN denotes DNA
T11765 2250-2253 VBD denotes was
T11760 2254-2262 VBN denotes prepared
T11766 2263-2265 IN denotes by
T11767 2266-2270 NN denotes CsCl
T11768 2271-2281 NN denotes separation
T11769 2281-2283 , denotes ,
T11770 2283-2291 VBN denotes digested
T11771 2292-2296 IN denotes with
T11772 2297-2301 NN denotes NotI
T11773 2302-2304 TO denotes to
T11774 2305-2309 VB denotes free
T11775 2310-2313 DT denotes the
T11776 2314-2320 NN denotes insert
T11777 2321-2325 IN denotes from
T11778 2326-2329 DT denotes the
T11779 2330-2336 NN denotes vector
T11780 2336-2338 , denotes ,
T11781 2338-2341 CC denotes and
T11782 2342-2346 NN denotes size
T11784 2346-2347 HYPH denotes -
T11783 2347-2359 VBN denotes fractionated
T11785 2360-2364 IN denotes over
T11786 2365-2366 DT denotes a
T11788 2367-2374 NN denotes sucrose
T11787 2375-2383 NN denotes gradient
T11789 2383-2384 . denotes .
T11790 2384-2495 sentence denotes Aliquots of fractions were run on a pulse-field gel and Southern blotted using vector-specific DNA as a probe.
T11791 2385-2393 NNS denotes Aliquots
T11793 2394-2396 IN denotes of
T11794 2397-2406 NNS denotes fractions
T11795 2407-2411 VBD denotes were
T11792 2412-2415 VBN denotes run
T11796 2416-2418 IN denotes on
T11797 2419-2420 DT denotes a
T11799 2421-2426 NN denotes pulse
T11801 2426-2427 HYPH denotes -
T11800 2427-2432 NN denotes field
T11798 2433-2436 NN denotes gel
T11802 2437-2440 CC denotes and
T11803 2441-2449 NNP denotes Southern
T11804 2450-2457 VBN denotes blotted
T11805 2458-2463 VBG denotes using
T11806 2464-2470 NN denotes vector
T11808 2470-2471 HYPH denotes -
T11809 2471-2479 JJ denotes specific
T11807 2480-2483 NN denotes DNA
T11810 2484-2486 IN denotes as
T11811 2487-2488 DT denotes a
T11812 2489-2494 NN denotes probe
T11813 2494-2495 . denotes .
T11814 2495-2745 sentence denotes Fractions containing unsheared insert and almost no detectable vector DNA were dialyzed in microinjection buffer (10 mM Tris [pH 7.4] with 0.15 mM EDTA [pH 8.0]) using Centriprep-30 concentrators (Millipore, Billerica, Massachusetts, United States).
T11815 2496-2505 NNS denotes Fractions
T11817 2506-2516 VBG denotes containing
T11818 2517-2526 JJ denotes unsheared
T11819 2527-2533 NN denotes insert
T11820 2534-2537 CC denotes and
T11821 2538-2544 RB denotes almost
T11823 2545-2547 DT denotes no
T11824 2548-2558 JJ denotes detectable
T11825 2559-2565 NN denotes vector
T11822 2566-2569 NN denotes DNA
T11826 2570-2574 VBD denotes were
T11816 2575-2583 VBN denotes dialyzed
T11827 2584-2586 IN denotes in
T11828 2587-2601 NN denotes microinjection
T11829 2602-2608 NN denotes buffer
T11830 2609-2610 -LRB- denotes (
T11832 2610-2612 CD denotes 10
T11833 2613-2615 NN denotes mM
T11831 2616-2620 NN denotes Tris
T11834 2621-2622 -LRB- denotes [
T11835 2622-2624 NN denotes pH
T11836 2625-2628 CD denotes 7.4
T11837 2628-2629 -RRB- denotes ]
T11838 2630-2634 IN denotes with
T11839 2635-2639 CD denotes 0.15
T11840 2640-2642 NN denotes mM
T11841 2643-2647 NN denotes EDTA
T11842 2648-2649 -LRB- denotes [
T11843 2649-2651 NN denotes pH
T11844 2652-2655 CD denotes 8.0
T11845 2655-2656 -RRB- denotes ]
T11846 2656-2657 -RRB- denotes )
T11847 2658-2663 VBG denotes using
T11848 2664-2674 NN denotes Centriprep
T11850 2674-2675 HYPH denotes -
T11851 2675-2677 CD denotes 30
T11849 2678-2691 NNS denotes concentrators
T11852 2692-2693 -LRB- denotes (
T11853 2693-2702 NNP denotes Millipore
T11854 2702-2704 , denotes ,
T11855 2704-2713 NNP denotes Billerica
T11856 2713-2715 , denotes ,
T11857 2715-2728 NNP denotes Massachusetts
T11858 2728-2730 , denotes ,
T11859 2730-2736 NNP denotes United
T11860 2737-2743 NNP denotes States
T11861 2743-2744 -RRB- denotes )
T11862 2744-2745 . denotes .
T11863 2745-2899 sentence denotes This purified insert DNA was adjusted to 1 ng/μl and injected into the pronucleus of fertilized eggs from FVB/N mice by the Stanford Transgenic Facility.
T11864 2746-2750 DT denotes This
T11866 2751-2759 VBN denotes purified
T11867 2760-2766 NN denotes insert
T11865 2767-2770 NN denotes DNA
T11869 2771-2774 VBD denotes was
T11868 2775-2783 VBN denotes adjusted
T11870 2784-2786 IN denotes to
T11871 2787-2788 CD denotes 1
T11872 2789-2791 NN denotes ng
T11873 2791-2792 SYM denotes /
T11874 2792-2794 NN denotes μl
T11875 2795-2798 CC denotes and
T11876 2799-2807 VBN denotes injected
T11877 2808-2812 IN denotes into
T11878 2813-2816 DT denotes the
T11879 2817-2827 NN denotes pronucleus
T11880 2828-2830 IN denotes of
T11881 2831-2841 VBN denotes fertilized
T11882 2842-2846 NNS denotes eggs
T11883 2847-2851 IN denotes from
T11884 2852-2855 NN denotes FVB
T11886 2855-2856 HYPH denotes /
T11885 2856-2857 NN denotes N
T11887 2858-2862 NNS denotes mice
T11888 2863-2865 IN denotes by
T11889 2866-2869 DT denotes the
T11891 2870-2878 NNP denotes Stanford
T11892 2879-2889 NNP denotes Transgenic
T11890 2890-2898 NNP denotes Facility
T11893 2898-2899 . denotes .
T11894 2899-3248 sentence denotes Transgenic founder mice were identified by PCR using Cre-specific primers 5′-GCCTGCATTACCGGTCGATGCAACGA-3′ and 5′-GTGGCAGATGGCGCGGCAACACCATT-3′, which amplify a 725-bp product, and were assessed for absence of BAC vector using vector-specific primers 5′-CGGAGTCTGATGCGGTTGCGATG-3′ and 5′-AGTGCTGTTCCCTGGTGCTTCCTC-3′, which amplify a 465-bp product.
T11895 2900-2910 JJ denotes Transgenic
T11897 2911-2918 NN denotes founder
T11896 2919-2923 NNS denotes mice
T11899 2924-2928 VBD denotes were
T11898 2929-2939 VBN denotes identified
T11900 2940-2942 IN denotes by
T11901 2943-2946 NN denotes PCR
T11902 2947-2952 VBG denotes using
T11903 2953-2956 NN denotes Cre
T11905 2956-2957 HYPH denotes -
T11904 2957-2965 JJ denotes specific
T11907 2966-2973 NNS denotes primers
T11908 2974-2975 CD denotes 5
T11909 2975-2976 SYM denotes
T11910 2976-2977 HYPH denotes -
T11906 2977-3003 NN denotes GCCTGCATTACCGGTCGATGCAACGA
T11911 3003-3004 HYPH denotes -
T11912 3004-3005 CD denotes 3
T11913 3005-3006 SYM denotes
T11914 3007-3010 CC denotes and
T11915 3011-3012 CD denotes 5
T11917 3012-3013 SYM denotes
T11918 3013-3014 HYPH denotes -
T11916 3014-3040 NN denotes GTGGCAGATGGCGCGGCAACACCATT
T11919 3040-3041 HYPH denotes -
T11920 3041-3042 CD denotes 3
T11921 3042-3043 SYM denotes
T11922 3043-3045 , denotes ,
T11923 3045-3050 WDT denotes which
T11924 3051-3058 VBP denotes amplify
T11925 3059-3060 DT denotes a
T11927 3061-3064 CD denotes 725
T11929 3064-3065 HYPH denotes -
T11928 3065-3067 NN denotes bp
T11926 3068-3075 NN denotes product
T11930 3075-3077 , denotes ,
T11931 3077-3080 CC denotes and
T11932 3081-3085 VBD denotes were
T11933 3086-3094 VBN denotes assessed
T11934 3095-3098 IN denotes for
T11935 3099-3106 NN denotes absence
T11936 3107-3109 IN denotes of
T11937 3110-3113 NN denotes BAC
T11938 3114-3120 NN denotes vector
T11939 3121-3126 VBG denotes using
T11940 3127-3133 NN denotes vector
T11942 3133-3134 HYPH denotes -
T11943 3134-3142 JJ denotes specific
T11941 3143-3150 NNS denotes primers
T11944 3151-3152 CD denotes 5
T11946 3152-3153 SYM denotes
T11947 3153-3154 HYPH denotes -
T11945 3154-3177 NN denotes CGGAGTCTGATGCGGTTGCGATG
T11948 3177-3178 HYPH denotes -
T11949 3178-3179 CD denotes 3
T11950 3179-3180 SYM denotes
T11951 3181-3184 CC denotes and
T11952 3185-3186 CD denotes 5
T11954 3186-3187 SYM denotes
T11955 3187-3188 HYPH denotes -
T11953 3188-3212 NN denotes AGTGCTGTTCCCTGGTGCTTCCTC
T11956 3212-3213 HYPH denotes -
T11957 3213-3214 CD denotes 3
T11958 3214-3215 SYM denotes
T11959 3215-3217 , denotes ,
T11960 3217-3222 WDT denotes which
T11961 3223-3230 VBP denotes amplify
T11962 3231-3232 DT denotes a
T11964 3233-3236 CD denotes 465
T11966 3236-3237 HYPH denotes -
T11965 3237-3239 NN denotes bp
T11963 3240-3247 NN denotes product
T11967 3247-3248 . denotes .
T11968 3248-3332 sentence denotes Three lines of Gdf5-Cre mice were established and maintained on the FVB background.
T11969 3249-3254 CD denotes Three
T11970 3255-3260 NNS denotes lines
T11972 3261-3263 IN denotes of
T11973 3264-3268 NN denotes Gdf5
T11975 3268-3269 HYPH denotes -
T11974 3269-3272 NN denotes Cre
T11976 3273-3277 NNS denotes mice
T11977 3278-3282 VBD denotes were
T11971 3283-3294 VBN denotes established
T11978 3295-3298 CC denotes and
T11979 3299-3309 VBN denotes maintained
T11980 3310-3312 IN denotes on
T11981 3313-3316 DT denotes the
T11983 3317-3320 NN denotes FVB
T11982 3321-3331 NN denotes background
T11984 3331-3332 . denotes .
T11985 3332-3434 sentence denotes Matings with R26R Cre-inducible LACZ reporter mice (Soriano 1999) were used to test for Cre activity.
T11986 3333-3340 NNS denotes Matings
T11988 3341-3345 IN denotes with
T11989 3346-3350 NN denotes R26R
T11990 3351-3354 NN denotes Cre
T11992 3354-3355 HYPH denotes -
T11991 3355-3364 JJ denotes inducible
T11994 3365-3369 NN denotes LACZ
T11995 3370-3378 NN denotes reporter
T11993 3379-3383 NNS denotes mice
T11996 3384-3385 -LRB- denotes (
T11997 3385-3392 NNP denotes Soriano
T11998 3393-3397 CD denotes 1999
T11999 3397-3398 -RRB- denotes )
T12000 3399-3403 VBD denotes were
T11987 3404-3408 VBN denotes used
T12001 3409-3411 TO denotes to
T12002 3412-3416 VB denotes test
T12003 3417-3420 IN denotes for
T12004 3421-3424 NN denotes Cre
T12005 3425-3433 NN denotes activity
T12006 3433-3434 . denotes .
T12007 3434-3571 sentence denotes Staining for LACZ and HPLAP on whole embryos or sections of embryos was accomplished following established protocols (Lobe et al. 1999).
T12008 3435-3443 VBG denotes Staining
T12010 3444-3447 IN denotes for
T12011 3448-3452 NN denotes LACZ
T12012 3453-3456 CC denotes and
T12013 3457-3462 NN denotes HPLAP
T12014 3463-3465 IN denotes on
T12015 3466-3471 JJ denotes whole
T12016 3472-3479 NNS denotes embryos
T12017 3480-3482 CC denotes or
T12018 3483-3491 NNS denotes sections
T12019 3492-3494 IN denotes of
T12020 3495-3502 NNS denotes embryos
T12021 3503-3506 VBD denotes was
T12009 3507-3519 VBN denotes accomplished
T12022 3520-3529 VBG denotes following
T12023 3530-3541 VBN denotes established
T12024 3542-3551 NNS denotes protocols
T12025 3552-3553 -LRB- denotes (
T12026 3553-3557 NNP denotes Lobe
T12027 3558-3560 FW denotes et
T12028 3561-3564 FW denotes al.
T12029 3565-3569 CD denotes 1999
T12030 3569-3570 -RRB- denotes )
T12031 3570-3571 . denotes .
T12032 3571-3720 sentence denotes The red LACZ substrate (see Figure 1E) is 6-chloro-3-indoxyl-beta-D-galactopyranoside (Biosynth International, Naperville, Illinois, United States).
T12033 3572-3575 DT denotes The
T12035 3576-3579 JJ denotes red
T12036 3580-3584 NN denotes LACZ
T12034 3585-3594 NN denotes substrate
T12038 3595-3596 -LRB- denotes (
T12039 3596-3599 VB denotes see
T12040 3600-3606 NN denotes Figure
T12041 3607-3609 CD denotes 1E
T12042 3609-3610 -RRB- denotes )
T12037 3611-3613 VBZ denotes is
T12043 3614-3615 CD denotes 6
T12045 3615-3616 HYPH denotes -
T12046 3616-3622 NN denotes chloro
T12047 3622-3623 HYPH denotes -
T12048 3623-3624 CD denotes 3
T12049 3624-3625 HYPH denotes -
T12050 3625-3632 NN denotes indoxyl
T12051 3632-3633 HYPH denotes -
T12052 3633-3637 NN denotes beta
T12053 3637-3638 HYPH denotes -
T12054 3638-3639 NN denotes D
T12055 3639-3640 HYPH denotes -
T12044 3640-3657 NN denotes galactopyranoside
T12056 3658-3659 -LRB- denotes (
T12058 3659-3667 NNP denotes Biosynth
T12057 3668-3681 NNP denotes International
T12059 3681-3683 , denotes ,
T12060 3683-3693 NNP denotes Naperville
T12061 3693-3695 , denotes ,
T12062 3695-3703 NNP denotes Illinois
T12063 3703-3705 , denotes ,
T12064 3705-3711 NNP denotes United
T12065 3712-3718 NNP denotes States
T12066 3718-3719 -RRB- denotes )
T12067 3719-3720 . denotes .
T12177 3722-3729 JJ denotes General
T12178 3730-3746 NN denotes characterization
T12179 3747-3749 IN denotes of
T12180 3750-3756 NN denotes Bmpr1a
T12181 3757-3763 JJ denotes mutant
T12182 3764-3768 NNS denotes mice
T12183 3768-3925 sentence denotes Bmpr1a null and floxed alleles (Ahn et al. 2001; Mishina et al. 2002) were obtained on a mixed 129 and C57BL/6 background and maintained by random breeding.
T12184 3769-3775 NN denotes Bmpr1a
T12185 3776-3780 JJ denotes null
T12187 3781-3784 CC denotes and
T12188 3785-3791 VBN denotes floxed
T12186 3792-3799 NNS denotes alleles
T12190 3800-3801 -LRB- denotes (
T12191 3801-3804 NNP denotes Ahn
T12192 3805-3807 FW denotes et
T12193 3808-3811 FW denotes al.
T12194 3812-3816 CD denotes 2001
T12195 3816-3817 : denotes ;
T12196 3818-3825 NNP denotes Mishina
T12197 3826-3828 FW denotes et
T12198 3829-3832 FW denotes al.
T12199 3833-3837 CD denotes 2002
T12200 3837-3838 -RRB- denotes )
T12201 3839-3843 VBD denotes were
T12189 3844-3852 VBN denotes obtained
T12202 3853-3855 IN denotes on
T12203 3856-3857 DT denotes a
T12205 3858-3863 VBN denotes mixed
T12206 3864-3867 CD denotes 129
T12207 3868-3871 CC denotes and
T12208 3872-3877 NN denotes C57BL
T12209 3877-3878 HYPH denotes /
T12210 3878-3879 CD denotes 6
T12204 3880-3890 NN denotes background
T12211 3891-3894 CC denotes and
T12212 3895-3905 VBN denotes maintained
T12213 3906-3908 IN denotes by
T12214 3909-3915 JJ denotes random
T12215 3916-3924 NN denotes breeding
T12216 3924-3925 . denotes .
T12217 3925-4027 sentence denotes Mice carrying the null and floxed alleles were typically mated to Gdf5-Cre mice as shown in Figure 3.
T12218 3926-3930 NNS denotes Mice
T12220 3931-3939 VBG denotes carrying
T12221 3940-3943 DT denotes the
T12223 3944-3948 JJ denotes null
T12224 3949-3952 CC denotes and
T12225 3953-3959 VBN denotes floxed
T12222 3960-3967 NNS denotes alleles
T12226 3968-3972 VBD denotes were
T12227 3973-3982 RB denotes typically
T12219 3983-3988 VBN denotes mated
T12228 3989-3991 IN denotes to
T12229 3992-3996 NN denotes Gdf5
T12231 3996-3997 HYPH denotes -
T12230 3997-4000 NN denotes Cre
T12232 4001-4005 NNS denotes mice
T12233 4006-4008 IN denotes as
T12234 4009-4014 VBN denotes shown
T12235 4015-4017 IN denotes in
T12236 4018-4024 NN denotes Figure
T12237 4025-4026 CD denotes 3
T12238 4026-4027 . denotes .
T12239 4027-4179 sentence denotes The resulting mice are on a mixed 129; C57Bl/6; FVB/N background, with both controls and mutant animals generated as littermates from the same matings.
T12240 4028-4031 DT denotes The
T12242 4032-4041 VBG denotes resulting
T12241 4042-4046 NNS denotes mice
T12243 4047-4050 VBP denotes are
T12244 4051-4053 IN denotes on
T12245 4054-4055 DT denotes a
T12247 4056-4061 VBN denotes mixed
T12248 4062-4065 CD denotes 129
T12249 4065-4066 : denotes ;
T12250 4067-4072 NN denotes C57Bl
T12251 4072-4073 HYPH denotes /
T12252 4073-4074 CD denotes 6
T12253 4074-4075 : denotes ;
T12254 4076-4079 NN denotes FVB
T12256 4079-4080 HYPH denotes /
T12255 4080-4081 NN denotes N
T12246 4082-4092 NN denotes background
T12257 4092-4094 , denotes ,
T12258 4094-4098 IN denotes with
T12260 4099-4103 CC denotes both
T12261 4104-4112 NNS denotes controls
T12262 4113-4116 CC denotes and
T12263 4117-4123 NN denotes mutant
T12264 4124-4131 NNS denotes animals
T12259 4132-4141 VBN denotes generated
T12265 4142-4144 IN denotes as
T12266 4145-4156 NNS denotes littermates
T12267 4157-4161 IN denotes from
T12268 4162-4165 DT denotes the
T12270 4166-4170 JJ denotes same
T12269 4171-4178 NNS denotes matings
T12271 4178-4179 . denotes .
T12272 4179-4271 sentence denotes Whole-mount skeletal preparations were made from 34- to 36-d-old mice (Lufkin et al. 1992).
T12273 4180-4185 JJ denotes Whole
T12275 4185-4186 HYPH denotes -
T12274 4186-4191 NN denotes mount
T12277 4192-4200 JJ denotes skeletal
T12276 4201-4213 NNS denotes preparations
T12279 4214-4218 VBD denotes were
T12278 4219-4223 VBN denotes made
T12280 4224-4228 IN denotes from
T12281 4229-4231 CD denotes 34
T12283 4231-4232 HYPH denotes -
T12284 4233-4235 IN denotes to
T12282 4236-4238 CD denotes 36
T12286 4238-4239 HYPH denotes -
T12285 4239-4240 NN denotes d
T12288 4240-4241 HYPH denotes -
T12287 4241-4244 JJ denotes old
T12289 4245-4249 NNS denotes mice
T12290 4250-4251 -LRB- denotes (
T12291 4251-4257 NNP denotes Lufkin
T12292 4258-4260 FW denotes et
T12293 4261-4264 FW denotes al.
T12294 4265-4269 CD denotes 1992
T12295 4269-4270 -RRB- denotes )
T12296 4270-4271 . denotes .
T12297 4271-4495 sentence denotes Pairs of ears from euthanized 6-mo-old animals were removed, pinned, photographed, projected, and measured from the base of the curve formed between the tragus and antitragus to the farthest point at the edge of the pinnae.
T12298 4272-4277 NNS denotes Pairs
T12300 4278-4280 IN denotes of
T12301 4281-4285 NNS denotes ears
T12302 4286-4290 IN denotes from
T12303 4291-4301 VBN denotes euthanized
T12305 4302-4303 CD denotes 6
T12307 4303-4304 HYPH denotes -
T12306 4304-4306 NN denotes mo
T12309 4306-4307 HYPH denotes -
T12308 4307-4310 JJ denotes old
T12304 4311-4318 NNS denotes animals
T12310 4319-4323 VBD denotes were
T12299 4324-4331 VBN denotes removed
T12311 4331-4333 , denotes ,
T12312 4333-4339 VBN denotes pinned
T12313 4339-4341 , denotes ,
T12314 4341-4353 VBN denotes photographed
T12315 4353-4355 , denotes ,
T12316 4355-4364 VBN denotes projected
T12317 4364-4366 , denotes ,
T12318 4366-4369 CC denotes and
T12319 4370-4378 VBN denotes measured
T12320 4379-4383 IN denotes from
T12321 4384-4387 DT denotes the
T12322 4388-4392 NN denotes base
T12323 4393-4395 IN denotes of
T12324 4396-4399 DT denotes the
T12325 4400-4405 NN denotes curve
T12326 4406-4412 VBN denotes formed
T12327 4413-4420 IN denotes between
T12328 4421-4424 DT denotes the
T12329 4425-4431 NN denotes tragus
T12330 4432-4435 CC denotes and
T12331 4436-4446 NN denotes antitragus
T12332 4447-4449 IN denotes to
T12333 4450-4453 DT denotes the
T12335 4454-4462 JJS denotes farthest
T12334 4463-4468 NN denotes point
T12336 4469-4471 IN denotes at
T12337 4472-4475 DT denotes the
T12338 4476-4480 NN denotes edge
T12339 4481-4483 IN denotes of
T12340 4484-4487 DT denotes the
T12341 4488-4494 NNS denotes pinnae
T12342 4494-4495 . denotes .
T12343 4495-4674 sentence denotes Grasping ability in 6-mo-old mice was measured by placing animals on a slender rod and timing how long they could remain suspended on the rod, to a maximum time allowed of 2 min.
T12344 4496-4504 NN denotes Grasping
T12345 4505-4512 NN denotes ability
T12347 4513-4515 IN denotes in
T12348 4516-4517 CD denotes 6
T12350 4517-4518 HYPH denotes -
T12349 4518-4520 NN denotes mo
T12352 4520-4521 HYPH denotes -
T12351 4521-4524 JJ denotes old
T12353 4525-4529 NNS denotes mice
T12354 4530-4533 VBD denotes was
T12346 4534-4542 VBN denotes measured
T12355 4543-4545 IN denotes by
T12356 4546-4553 VBG denotes placing
T12357 4554-4561 NNS denotes animals
T12358 4562-4564 IN denotes on
T12359 4565-4566 DT denotes a
T12361 4567-4574 JJ denotes slender
T12360 4575-4578 NN denotes rod
T12362 4579-4582 CC denotes and
T12363 4583-4589 VBG denotes timing
T12364 4590-4593 WRB denotes how
T12365 4594-4598 RB denotes long
T12367 4599-4603 PRP denotes they
T12368 4604-4609 MD denotes could
T12366 4610-4616 VB denotes remain
T12369 4617-4626 JJ denotes suspended
T12370 4627-4629 IN denotes on
T12371 4630-4633 DT denotes the
T12372 4634-4637 NN denotes rod
T12373 4637-4639 , denotes ,
T12374 4639-4641 IN denotes to
T12375 4642-4643 DT denotes a
T12377 4644-4651 JJ denotes maximum
T12376 4652-4656 NN denotes time
T12378 4657-4664 VBN denotes allowed
T12379 4665-4667 IN denotes of
T12380 4668-4669 CD denotes 2
T12381 4670-4673 NN denotes min
T12382 4673-4674 . denotes .
T12383 4674-4738 sentence denotes Data from five consecutive trials for each mouse were averaged.
T12384 4675-4679 NNS denotes Data
T12386 4680-4684 IN denotes from
T12387 4685-4689 CD denotes five
T12389 4690-4701 JJ denotes consecutive
T12388 4702-4708 NNS denotes trials
T12390 4709-4712 IN denotes for
T12391 4713-4717 DT denotes each
T12392 4718-4723 NN denotes mouse
T12393 4724-4728 VBD denotes were
T12385 4729-4737 VBN denotes averaged
T12394 4737-4738 . denotes .
T12395 4738-4870 sentence denotes Range of motion assays were conducted on the MT/P1 and P1/P2 joints of the second hindlimb digit from euthanized 18-wk-old animals.
T12396 4739-4744 NN denotes Range
T12398 4745-4747 IN denotes of
T12399 4748-4754 NN denotes motion
T12397 4755-4761 NNS denotes assays
T12401 4762-4766 VBD denotes were
T12400 4767-4776 VBN denotes conducted
T12402 4777-4779 IN denotes on
T12403 4780-4783 DT denotes the
T12405 4784-4786 NN denotes MT
T12407 4786-4787 HYPH denotes /
T12406 4787-4789 NN denotes P1
T12408 4790-4793 CC denotes and
T12409 4794-4796 NN denotes P1
T12411 4796-4797 HYPH denotes /
T12410 4797-4799 NN denotes P2
T12404 4800-4806 NNS denotes joints
T12412 4807-4809 IN denotes of
T12413 4810-4813 DT denotes the
T12415 4814-4820 JJ denotes second
T12416 4821-4829 NN denotes hindlimb
T12414 4830-4835 NN denotes digit
T12417 4836-4840 IN denotes from
T12418 4841-4851 VBN denotes euthanized
T12420 4852-4854 CD denotes 18
T12422 4854-4855 HYPH denotes -
T12421 4855-4857 NN denotes wk
T12424 4857-4858 HYPH denotes -
T12423 4858-4861 JJ denotes old
T12419 4862-4869 NNS denotes animals
T12425 4869-4870 . denotes .
T12426 4870-5045 sentence denotes Forceps were used to bend the joint to its natural stopping position, and the resulting angle was measured to the nearest 10° under 12.5× magnification using a 360° reticule.
T12427 4871-4878 NNS denotes Forceps
T12429 4879-4883 VBD denotes were
T12428 4884-4888 VBN denotes used
T12430 4889-4891 TO denotes to
T12431 4892-4896 VB denotes bend
T12432 4897-4900 DT denotes the
T12433 4901-4906 NN denotes joint
T12434 4907-4909 IN denotes to
T12435 4910-4913 PRP$ denotes its
T12437 4914-4921 JJ denotes natural
T12438 4922-4930 NN denotes stopping
T12436 4931-4939 NN denotes position
T12439 4939-4941 , denotes ,
T12440 4941-4944 CC denotes and
T12441 4945-4948 DT denotes the
T12443 4949-4958 VBG denotes resulting
T12442 4959-4964 NN denotes angle
T12445 4965-4968 VBD denotes was
T12444 4969-4977 VBN denotes measured
T12446 4978-4980 IN denotes to
T12447 4981-4984 DT denotes the
T12449 4985-4992 JJS denotes nearest
T12450 4993-4995 CD denotes 10
T12448 4995-4996 NNS denotes °
T12451 4997-5002 IN denotes under
T12452 5003-5007 CD denotes 12.5
T12454 5007-5008 SYM denotes ×
T12453 5009-5022 NN denotes magnification
T12455 5023-5028 VBG denotes using
T12456 5029-5030 DT denotes a
T12458 5031-5034 CD denotes 360
T12459 5034-5035 NN denotes °
T12457 5036-5044 NN denotes reticule
T12460 5044-5045 . denotes .
T12461 5045-5114 sentence denotes Analysis described in this section occurred on animals lacking R26R.
T12462 5046-5054 NN denotes Analysis
T12464 5055-5064 VBN denotes described
T12465 5065-5067 IN denotes in
T12466 5068-5072 DT denotes this
T12467 5073-5080 NN denotes section
T12463 5081-5089 VBD denotes occurred
T12468 5090-5092 IN denotes on
T12469 5093-5100 NNS denotes animals
T12470 5101-5108 VBG denotes lacking
T12471 5109-5113 NN denotes R26R
T12472 5113-5114 . denotes .
T12473 5114-5293 sentence denotes Control mice included all nonmutant genotypes generated by Parent 1 being heterozygous for Gdf5-Cre and Bmpr1anull and Parent 2 being heterozygous for Bmpr1afloxP (see Figure 3).
T12474 5115-5122 NN denotes Control
T12475 5123-5127 NNS denotes mice
T12476 5128-5136 VBD denotes included
T12477 5137-5140 DT denotes all
T12479 5141-5150 JJ denotes nonmutant
T12478 5151-5160 NNS denotes genotypes
T12480 5161-5170 VBN denotes generated
T12481 5171-5173 IN denotes by
T12482 5174-5180 NN denotes Parent
T12484 5181-5182 CD denotes 1
T12483 5183-5188 VBG denotes being
T12485 5189-5201 JJ denotes heterozygous
T12486 5202-5205 IN denotes for
T12487 5206-5210 NN denotes Gdf5
T12489 5210-5211 HYPH denotes -
T12488 5211-5214 NN denotes Cre
T12490 5215-5218 CC denotes and
T12491 5219-5229 NN denotes Bmpr1anull
T12492 5230-5233 CC denotes and
T12493 5234-5240 NN denotes Parent
T12495 5241-5242 CD denotes 2
T12494 5243-5248 VBG denotes being
T12496 5249-5261 JJ denotes heterozygous
T12497 5262-5265 IN denotes for
T12498 5266-5277 NN denotes Bmpr1afloxP
T12499 5278-5279 -LRB- denotes (
T12500 5279-5282 VB denotes see
T12501 5283-5289 NN denotes Figure
T12502 5290-5291 CD denotes 3
T12503 5291-5292 -RRB- denotes )
T12504 5292-5293 . denotes .
T12505 5293-5420 sentence denotes All statistical analysis used the Student's t-test or Welch's t-test, and values listed are mean ± standard error of the mean.
T12506 5294-5297 DT denotes All
T12508 5298-5309 JJ denotes statistical
T12507 5310-5318 NN denotes analysis
T12509 5319-5323 VBD denotes used
T12510 5324-5327 DT denotes the
T12511 5328-5335 NNP denotes Student
T12513 5335-5337 POS denotes 's
T12514 5338-5339 NN denotes t
T12515 5339-5340 HYPH denotes -
T12512 5340-5344 NN denotes test
T12516 5345-5347 CC denotes or
T12517 5348-5353 NNP denotes Welch
T12519 5353-5355 POS denotes 's
T12520 5356-5357 NN denotes t
T12521 5357-5358 HYPH denotes -
T12518 5358-5362 NN denotes test
T12522 5362-5364 , denotes ,
T12523 5364-5367 CC denotes and
T12524 5368-5374 NNS denotes values
T12526 5375-5381 VBN denotes listed
T12525 5382-5385 VBP denotes are
T12527 5386-5390 JJ denotes mean
T12528 5391-5392 SYM denotes ±
T12529 5393-5401 JJ denotes standard
T12530 5402-5407 NN denotes error
T12531 5408-5410 IN denotes of
T12532 5411-5414 DT denotes the
T12533 5415-5419 NN denotes mean
T12534 5419-5420 . denotes .
T12651 5422-5426 NN denotes Cell
T12652 5427-5432 NN denotes death
T12653 5433-5436 CC denotes and
T12654 5437-5450 NN denotes proliferation
T12655 5451-5457 NNS denotes assays
T12656 5457-5593 sentence denotes Limbs from mutant and control animals at E13.5 and E14.5 were dissected and frozen in OCT (Sakura Finetek,Torrence, CA, United States).
T12657 5458-5463 NNS denotes Limbs
T12659 5464-5468 IN denotes from
T12660 5469-5475 JJ denotes mutant
T12662 5476-5479 CC denotes and
T12663 5480-5487 NN denotes control
T12661 5488-5495 NNS denotes animals
T12664 5496-5498 IN denotes at
T12665 5499-5504 NN denotes E13.5
T12666 5505-5508 CC denotes and
T12667 5509-5514 NN denotes E14.5
T12668 5515-5519 VBD denotes were
T12658 5520-5529 VBN denotes dissected
T12669 5530-5533 CC denotes and
T12670 5534-5540 VBN denotes frozen
T12671 5541-5543 IN denotes in
T12672 5544-5547 NN denotes OCT
T12673 5548-5549 -LRB- denotes (
T12675 5549-5555 NNP denotes Sakura
T12674 5556-5563 NNP denotes Finetek
T12676 5563-5564 , denotes ,
T12677 5564-5572 NNP denotes Torrence
T12678 5572-5574 , denotes ,
T12679 5574-5576 NNP denotes CA
T12680 5576-5578 , denotes ,
T12681 5578-5584 NNP denotes United
T12682 5585-5591 NNP denotes States
T12683 5591-5592 -RRB- denotes )
T12684 5592-5593 . denotes .
T12685 5593-5723 sentence denotes Cryosections of tissue were assayed by TUNEL using the In Situ Cell Death Detection Kit, Fluorescein (Roche, Basel, Switzerland).
T12686 5594-5606 NNS denotes Cryosections
T12688 5607-5609 IN denotes of
T12689 5610-5616 NN denotes tissue
T12690 5617-5621 VBD denotes were
T12687 5622-5629 VBN denotes assayed
T12691 5630-5632 IN denotes by
T12692 5633-5638 NN denotes TUNEL
T12693 5639-5644 VBG denotes using
T12694 5645-5648 DT denotes the
T12696 5649-5651 FW denotes In
T12697 5652-5656 FW denotes Situ
T12699 5657-5661 NN denotes Cell
T12700 5662-5667 NN denotes Death
T12698 5668-5677 NN denotes Detection
T12695 5678-5681 NN denotes Kit
T12701 5681-5683 , denotes ,
T12702 5683-5694 NNP denotes Fluorescein
T12703 5695-5696 -LRB- denotes (
T12704 5696-5701 NNP denotes Roche
T12705 5701-5703 , denotes ,
T12706 5703-5708 NNP denotes Basel
T12707 5708-5710 , denotes ,
T12708 5710-5721 NNP denotes Switzerland
T12709 5721-5722 -RRB- denotes )
T12710 5722-5723 . denotes .
T12711 5723-6027 sentence denotes Following TUNEL, slides were washed in PBS, blocked with PBS + 0.05% Tween-20 + 5% goat serum, washed again, and incubated with a 1:200 dilution of a rabbit anti-phospho-histone-H3 antibody called Mitosis Marker (Upstate Biotechnology, Lake Placid, New York, United States) to identify cells in mitosis.
T12712 5724-5733 VBG denotes Following
T12714 5734-5739 NN denotes TUNEL
T12715 5739-5741 , denotes ,
T12716 5741-5747 NNS denotes slides
T12717 5748-5752 VBD denotes were
T12713 5753-5759 VBN denotes washed
T12718 5760-5762 IN denotes in
T12719 5763-5766 NN denotes PBS
T12720 5766-5768 , denotes ,
T12721 5768-5775 VBN denotes blocked
T12722 5776-5780 IN denotes with
T12723 5781-5784 NN denotes PBS
T12724 5785-5786 SYM denotes +
T12725 5787-5791 CD denotes 0.05
T12726 5791-5792 NN denotes %
T12727 5793-5798 NN denotes Tween
T12728 5798-5799 HYPH denotes -
T12729 5799-5801 CD denotes 20
T12730 5802-5803 SYM denotes +
T12731 5804-5805 CD denotes 5
T12732 5805-5806 NN denotes %
T12734 5807-5811 NN denotes goat
T12733 5812-5817 NN denotes serum
T12735 5817-5819 , denotes ,
T12736 5819-5825 VBD denotes washed
T12737 5826-5831 RB denotes again
T12738 5831-5833 , denotes ,
T12739 5833-5836 CC denotes and
T12740 5837-5846 VBN denotes incubated
T12741 5847-5851 IN denotes with
T12742 5852-5853 DT denotes a
T12744 5854-5855 CD denotes 1
T12745 5855-5856 SYM denotes :
T12746 5856-5859 CD denotes 200
T12743 5860-5868 NN denotes dilution
T12747 5869-5871 IN denotes of
T12748 5872-5873 DT denotes a
T12750 5874-5880 NN denotes rabbit
T12751 5881-5901 JJ denotes anti-phospho-histone
T12753 5901-5902 HYPH denotes -
T12752 5902-5904 NN denotes H3
T12749 5905-5913 NN denotes antibody
T12754 5914-5920 VBN denotes called
T12755 5921-5928 NN denotes Mitosis
T12756 5929-5935 NN denotes Marker
T12757 5936-5937 -LRB- denotes (
T12759 5937-5944 NNP denotes Upstate
T12758 5945-5958 NNP denotes Biotechnology
T12760 5958-5960 , denotes ,
T12761 5960-5964 NNP denotes Lake
T12762 5965-5971 NNP denotes Placid
T12763 5971-5973 , denotes ,
T12764 5973-5976 NNP denotes New
T12765 5977-5981 NNP denotes York
T12766 5981-5983 , denotes ,
T12767 5983-5989 NNP denotes United
T12768 5990-5996 NNP denotes States
T12769 5996-5997 -RRB- denotes )
T12770 5998-6000 TO denotes to
T12771 6001-6009 VB denotes identify
T12772 6010-6015 NNS denotes cells
T12773 6016-6018 IN denotes in
T12774 6019-6026 NN denotes mitosis
T12775 6026-6027 . denotes .
T12776 6027-6103 sentence denotes Cy3-labeled anti-rabbit secondary antibody was used to detect the antibody.
T12777 6028-6031 NN denotes Cy3
T12779 6031-6032 HYPH denotes -
T12778 6032-6039 VBN denotes labeled
T12781 6040-6051 JJ denotes anti-rabbit
T12782 6052-6061 JJ denotes secondary
T12780 6062-6070 NN denotes antibody
T12784 6071-6074 VBD denotes was
T12783 6075-6079 VBN denotes used
T12785 6080-6082 TO denotes to
T12786 6083-6089 VB denotes detect
T12787 6090-6093 DT denotes the
T12788 6094-6102 NN denotes antibody
T12789 6102-6103 . denotes .
T12790 6103-6276 sentence denotes Cell nuclei were labeled with DAPI, and slides were mounted in Vectamount (Vector Laboratories, Burlingame, California, United States) and visualized at 100× magnification.
T12791 6104-6108 NN denotes Cell
T12792 6109-6115 NNS denotes nuclei
T12794 6116-6120 VBD denotes were
T12793 6121-6128 VBN denotes labeled
T12795 6129-6133 IN denotes with
T12796 6134-6138 NN denotes DAPI
T12797 6138-6140 , denotes ,
T12798 6140-6143 CC denotes and
T12799 6144-6150 NNS denotes slides
T12801 6151-6155 VBD denotes were
T12800 6156-6163 VBN denotes mounted
T12802 6164-6166 IN denotes in
T12803 6167-6177 NNP denotes Vectamount
T12804 6178-6179 -LRB- denotes (
T12806 6179-6185 NNP denotes Vector
T12805 6186-6198 NNP denotes Laboratories
T12807 6198-6200 , denotes ,
T12808 6200-6210 NNP denotes Burlingame
T12809 6210-6212 , denotes ,
T12810 6212-6222 NNP denotes California
T12811 6222-6224 , denotes ,
T12812 6224-6230 NNP denotes United
T12813 6231-6237 NNP denotes States
T12814 6237-6238 -RRB- denotes )
T12815 6239-6242 CC denotes and
T12816 6243-6253 VBN denotes visualized
T12817 6254-6256 IN denotes at
T12818 6257-6260 CD denotes 100
T12820 6260-6261 SYM denotes ×
T12819 6262-6275 NN denotes magnification
T12821 6275-6276 . denotes .
T12822 6276-6516 sentence denotes The area of selected anatomical sites were measured, and the number of TUNEL-labeled nuclear fragments and the number of Cy3-labeled nuclei were counted from three 10-μm sections spanning 50 μm, from three control and three mutant animals.
T12823 6277-6280 DT denotes The
T12824 6281-6285 NN denotes area
T12826 6286-6288 IN denotes of
T12827 6289-6297 VBN denotes selected
T12829 6298-6308 JJ denotes anatomical
T12828 6309-6314 NNS denotes sites
T12830 6315-6319 VBD denotes were
T12825 6320-6328 VBN denotes measured
T12831 6328-6330 , denotes ,
T12832 6330-6333 CC denotes and
T12833 6334-6337 DT denotes the
T12834 6338-6344 NN denotes number
T12836 6345-6347 IN denotes of
T12837 6348-6353 NN denotes TUNEL
T12839 6353-6354 HYPH denotes -
T12838 6354-6361 VBN denotes labeled
T12841 6362-6369 JJ denotes nuclear
T12840 6370-6379 NNS denotes fragments
T12842 6380-6383 CC denotes and
T12843 6384-6387 DT denotes the
T12844 6388-6394 NN denotes number
T12845 6395-6397 IN denotes of
T12846 6398-6401 NN denotes Cy3
T12848 6401-6402 HYPH denotes -
T12847 6402-6409 VBN denotes labeled
T12849 6410-6416 NNS denotes nuclei
T12850 6417-6421 VBD denotes were
T12835 6422-6429 VBN denotes counted
T12851 6430-6434 IN denotes from
T12852 6435-6440 CD denotes three
T12854 6441-6443 CD denotes 10
T12856 6443-6444 HYPH denotes -
T12855 6444-6446 NN denotes μm
T12853 6447-6455 NNS denotes sections
T12857 6456-6464 VBG denotes spanning
T12858 6465-6467 CD denotes 50
T12859 6468-6470 NN denotes μm
T12860 6470-6472 , denotes ,
T12861 6472-6476 IN denotes from
T12862 6477-6482 CD denotes three
T12863 6483-6490 NN denotes control
T12864 6491-6494 CC denotes and
T12865 6495-6500 CD denotes three
T12866 6501-6507 NN denotes mutant
T12867 6508-6515 NNS denotes animals
T12868 6515-6516 . denotes .
T12869 6516-6688 sentence denotes The number of labeled cells in the metacarpal-phalangeal and metatarsal-phalangeal joints was counted in a 290 μm × 365 μm rectangle placed around the center of the joint.
T12870 6517-6520 DT denotes The
T12871 6521-6527 NN denotes number
T12873 6528-6530 IN denotes of
T12874 6531-6538 VBN denotes labeled
T12875 6539-6544 NNS denotes cells
T12876 6545-6547 IN denotes in
T12877 6548-6551 DT denotes the
T12879 6552-6562 JJ denotes metacarpal
T12881 6562-6563 HYPH denotes -
T12880 6563-6573 JJ denotes phalangeal
T12882 6574-6577 CC denotes and
T12883 6578-6588 JJ denotes metatarsal
T12885 6588-6589 HYPH denotes -
T12884 6589-6599 JJ denotes phalangeal
T12878 6600-6606 NNS denotes joints
T12886 6607-6610 VBD denotes was
T12872 6611-6618 VBN denotes counted
T12887 6619-6621 IN denotes in
T12888 6622-6623 DT denotes a
T12890 6624-6627 CD denotes 290
T12891 6628-6630 NN denotes μm
T12892 6631-6632 SYM denotes ×
T12894 6633-6636 CD denotes 365
T12893 6637-6639 NN denotes μm
T12889 6640-6649 NN denotes rectangle
T12895 6650-6656 VBN denotes placed
T12896 6657-6663 IN denotes around
T12897 6664-6667 DT denotes the
T12898 6668-6674 NN denotes center
T12899 6675-6677 IN denotes of
T12900 6678-6681 DT denotes the
T12901 6682-6687 NN denotes joint
T12902 6687-6688 . denotes .
T12903 6688-6843 sentence denotes The posterior region of the fifth digit was defined by drawing a line from the tip of the digit down 2.15 mm and across to the lateral edge of the tissue.
T12904 6689-6692 DT denotes The
T12906 6693-6702 JJ denotes posterior
T12905 6703-6709 NN denotes region
T12908 6710-6712 IN denotes of
T12909 6713-6716 DT denotes the
T12911 6717-6722 JJ denotes fifth
T12910 6723-6728 NN denotes digit
T12912 6729-6732 VBD denotes was
T12907 6733-6740 VBN denotes defined
T12913 6741-6743 IN denotes by
T12914 6744-6751 VBG denotes drawing
T12915 6752-6753 DT denotes a
T12916 6754-6758 NN denotes line
T12917 6759-6763 IN denotes from
T12918 6764-6767 DT denotes the
T12919 6768-6771 NN denotes tip
T12920 6772-6774 IN denotes of
T12921 6775-6778 DT denotes the
T12922 6779-6784 NN denotes digit
T12923 6785-6789 IN denotes down
T12924 6790-6794 CD denotes 2.15
T12925 6795-6797 NN denotes mm
T12926 6798-6801 CC denotes and
T12927 6802-6808 IN denotes across
T12928 6809-6811 IN denotes to
T12929 6812-6815 DT denotes the
T12931 6816-6823 JJ denotes lateral
T12930 6824-6828 NN denotes edge
T12932 6829-6831 IN denotes of
T12933 6832-6835 DT denotes the
T12934 6836-6842 NN denotes tissue
T12935 6842-6843 . denotes .
T12936 6843-6901 sentence denotes For this analysis, the R26R Cre reporter was not present.
T12937 6844-6847 IN denotes For
T12939 6848-6852 DT denotes this
T12940 6853-6861 NN denotes analysis
T12941 6861-6863 , denotes ,
T12942 6863-6866 DT denotes the
T12944 6867-6871 NN denotes R26R
T12945 6872-6875 NN denotes Cre
T12943 6876-6884 NN denotes reporter
T12938 6885-6888 VBD denotes was
T12946 6889-6892 RB denotes not
T12947 6893-6900 JJ denotes present
T12948 6900-6901 . denotes .
T13137 6903-6912 NN denotes Histology
T13138 6913-6916 CC denotes and
T13139 6917-6931 NN denotes histochemistry
T13140 6931-7262 sentence denotes Tissue from animals ranging from stages E14.5 to P14 was prepared for analysis by fixing in 4% paraformaldehyde (PFA) in PBS for 45 min to 4 h depending on the stage; washing three times in PBS, once in PBS + 15% sucrose for 1 h, and once in PBS + 30% sucrose for 2 h to overnight depending on the stage; and then freezing in OCT.
T13141 6932-6938 NN denotes Tissue
T13143 6939-6943 IN denotes from
T13144 6944-6951 NNS denotes animals
T13145 6952-6959 VBG denotes ranging
T13146 6960-6964 IN denotes from
T13147 6965-6971 NNS denotes stages
T13148 6972-6977 NN denotes E14.5
T13149 6978-6980 IN denotes to
T13150 6981-6984 NN denotes P14
T13151 6985-6988 VBD denotes was
T13142 6989-6997 VBN denotes prepared
T13152 6998-7001 IN denotes for
T13153 7002-7010 NN denotes analysis
T13154 7011-7013 IN denotes by
T13155 7014-7020 VBG denotes fixing
T13156 7021-7023 IN denotes in
T13157 7024-7025 CD denotes 4
T13158 7025-7026 NN denotes %
T13159 7027-7043 NN denotes paraformaldehyde
T13160 7044-7045 -LRB- denotes (
T13161 7045-7048 NN denotes PFA
T13162 7048-7049 -RRB- denotes )
T13163 7050-7052 IN denotes in
T13164 7053-7056 NN denotes PBS
T13165 7057-7060 IN denotes for
T13166 7061-7063 CD denotes 45
T13167 7064-7067 NN denotes min
T13168 7068-7070 IN denotes to
T13169 7071-7072 CD denotes 4
T13170 7073-7074 NN denotes h
T13171 7075-7084 VBG denotes depending
T13172 7085-7087 IN denotes on
T13173 7088-7091 DT denotes the
T13174 7092-7097 NN denotes stage
T13175 7097-7098 : denotes ;
T13176 7099-7106 VBG denotes washing
T13177 7107-7112 CD denotes three
T13178 7113-7118 NNS denotes times
T13179 7119-7121 IN denotes in
T13180 7122-7125 NN denotes PBS
T13181 7125-7127 , denotes ,
T13182 7127-7131 RB denotes once
T13183 7132-7134 IN denotes in
T13184 7135-7138 NN denotes PBS
T13185 7139-7140 SYM denotes +
T13186 7141-7143 CD denotes 15
T13187 7143-7144 NN denotes %
T13188 7145-7152 NN denotes sucrose
T13189 7153-7156 IN denotes for
T13190 7157-7158 CD denotes 1
T13191 7159-7160 NN denotes h
T13192 7160-7162 , denotes ,
T13193 7162-7165 CC denotes and
T13194 7166-7170 RB denotes once
T13195 7171-7173 IN denotes in
T13196 7174-7177 NN denotes PBS
T13197 7178-7179 SYM denotes +
T13198 7180-7182 CD denotes 30
T13199 7182-7183 NN denotes %
T13200 7184-7191 NN denotes sucrose
T13201 7192-7195 IN denotes for
T13202 7196-7197 CD denotes 2
T13203 7198-7199 NN denotes h
T13204 7200-7202 IN denotes to
T13205 7203-7212 NN denotes overnight
T13206 7213-7222 VBG denotes depending
T13207 7223-7225 IN denotes on
T13208 7226-7229 DT denotes the
T13209 7230-7235 NN denotes stage
T13210 7235-7236 : denotes ;
T13211 7237-7240 CC denotes and
T13212 7241-7245 RB denotes then
T13213 7246-7254 VBG denotes freezing
T13214 7255-7257 IN denotes in
T13215 7258-7261 NN denotes OCT
T13216 7261-7262 . denotes .
T13217 7262-7436 sentence denotes Tissue from animals aged 7 wk to 9 mo was processed similarly to earlier stages except that it was decalcified in 0.5 M EDTA (pH 7.4) for 4 d prior to incubating in sucrose.
T13218 7263-7269 NN denotes Tissue
T13220 7270-7274 IN denotes from
T13221 7275-7282 NNS denotes animals
T13222 7283-7287 VBN denotes aged
T13223 7288-7289 CD denotes 7
T13224 7290-7292 NNS denotes wk
T13225 7293-7295 IN denotes to
T13226 7296-7297 CD denotes 9
T13227 7298-7300 NNS denotes mo
T13228 7301-7304 VBD denotes was
T13219 7305-7314 VBN denotes processed
T13229 7315-7324 RB denotes similarly
T13230 7325-7327 IN denotes to
T13231 7328-7335 JJR denotes earlier
T13232 7336-7342 NNS denotes stages
T13233 7343-7349 IN denotes except
T13234 7350-7354 IN denotes that
T13236 7355-7357 PRP denotes it
T13237 7358-7361 VBD denotes was
T13235 7362-7373 VBN denotes decalcified
T13238 7374-7376 IN denotes in
T13239 7377-7380 CD denotes 0.5
T13240 7381-7382 NN denotes M
T13241 7383-7387 NN denotes EDTA
T13242 7388-7389 -LRB- denotes (
T13243 7389-7391 NN denotes pH
T13244 7392-7395 CD denotes 7.4
T13245 7395-7396 -RRB- denotes )
T13246 7397-7400 IN denotes for
T13247 7401-7402 CD denotes 4
T13248 7403-7404 NN denotes d
T13249 7405-7410 JJ denotes prior
T13250 7411-7413 IN denotes to
T13251 7414-7424 VBG denotes incubating
T13252 7425-7427 IN denotes in
T13253 7428-7435 NN denotes sucrose
T13254 7435-7436 . denotes .
T13255 7436-7587 sentence denotes All solutions were prechilled and used at 4 °C with agitation, and skin from tissues of P0 or older mice was lacerated or removed prior to processing.
T13256 7437-7440 DT denotes All
T13257 7441-7450 NNS denotes solutions
T13259 7451-7455 VBD denotes were
T13258 7456-7466 VBN denotes prechilled
T13260 7467-7470 CC denotes and
T13261 7471-7475 VBN denotes used
T13262 7476-7478 IN denotes at
T13263 7479-7480 CD denotes 4
T13264 7481-7483 NN denotes °C
T13265 7484-7488 IN denotes with
T13266 7489-7498 NN denotes agitation
T13267 7498-7500 , denotes ,
T13268 7500-7503 CC denotes and
T13269 7504-7508 NN denotes skin
T13271 7509-7513 IN denotes from
T13272 7514-7521 NNS denotes tissues
T13273 7522-7524 IN denotes of
T13274 7525-7527 NN denotes P0
T13276 7528-7530 CC denotes or
T13277 7531-7536 JJR denotes older
T13275 7537-7541 NNS denotes mice
T13278 7542-7545 VBD denotes was
T13270 7546-7555 VBN denotes lacerated
T13279 7556-7558 CC denotes or
T13280 7559-7566 VBN denotes removed
T13281 7567-7572 JJ denotes prior
T13282 7573-7575 IN denotes to
T13283 7576-7586 NN denotes processing
T13284 7586-7587 . denotes .
T13285 7587-7641 sentence denotes Tissue was then cryosectioned at 12 μm and processed.
T13286 7588-7594 NN denotes Tissue
T13288 7595-7598 VBD denotes was
T13289 7599-7603 RB denotes then
T13287 7604-7617 VBN denotes cryosectioned
T13290 7618-7620 IN denotes at
T13291 7621-7623 CD denotes 12
T13292 7624-7626 NN denotes μm
T13293 7627-7630 CC denotes and
T13294 7631-7640 VBN denotes processed
T13295 7640-7641 . denotes .
T13296 7641-7771 sentence denotes Staining of sections with Safranin O, Fast Green, and Harris' hematoxylin was carried out using standard histological procedures.
T13297 7642-7650 NN denotes Staining
T13299 7651-7653 IN denotes of
T13300 7654-7662 NNS denotes sections
T13301 7663-7667 IN denotes with
T13302 7668-7676 NN denotes Safranin
T13303 7677-7678 NN denotes O
T13304 7678-7680 , denotes ,
T13305 7680-7684 JJ denotes Fast
T13306 7685-7690 NN denotes Green
T13307 7690-7692 , denotes ,
T13308 7692-7695 CC denotes and
T13309 7696-7702 NNP denotes Harris
T13311 7702-7703 POS denotes '
T13310 7704-7715 NN denotes hematoxylin
T13312 7716-7719 VBD denotes was
T13298 7720-7727 VBN denotes carried
T13313 7728-7731 RP denotes out
T13314 7732-7737 VBG denotes using
T13315 7738-7746 JJ denotes standard
T13317 7747-7759 JJ denotes histological
T13316 7760-7770 NNS denotes procedures
T13318 7770-7771 . denotes .
T13319 7771-8091 sentence denotes Detection of LACZ activity with X-Gal was performed as described (Lobe et al. 1999) and was followed by refixing in 4% PFA, rinsing with deionized water, counterstaining with Nuclear Fast Red (Vector Labs), rinsing with water again, and then mounting in Aquamount (Lerner Labs, Pittsburgh, Pennsylvania, United States).
T13320 7772-7781 NN denotes Detection
T13322 7782-7784 IN denotes of
T13323 7785-7789 NN denotes LACZ
T13324 7790-7798 NN denotes activity
T13325 7799-7803 IN denotes with
T13326 7804-7805 NN denotes X
T13328 7805-7806 HYPH denotes -
T13327 7806-7809 NN denotes Gal
T13329 7810-7813 VBD denotes was
T13321 7814-7823 VBN denotes performed
T13330 7824-7826 IN denotes as
T13331 7827-7836 VBN denotes described
T13332 7837-7838 -LRB- denotes (
T13333 7838-7842 NNP denotes Lobe
T13334 7843-7845 FW denotes et
T13335 7846-7849 FW denotes al.
T13336 7850-7854 CD denotes 1999
T13337 7854-7855 -RRB- denotes )
T13338 7856-7859 CC denotes and
T13339 7860-7863 VBD denotes was
T13340 7864-7872 VBN denotes followed
T13341 7873-7875 IN denotes by
T13342 7876-7884 VBG denotes refixing
T13343 7885-7887 IN denotes in
T13344 7888-7889 CD denotes 4
T13345 7889-7890 NN denotes %
T13346 7891-7894 NN denotes PFA
T13347 7894-7896 , denotes ,
T13348 7896-7903 VBG denotes rinsing
T13349 7904-7908 IN denotes with
T13350 7909-7918 VBN denotes deionized
T13351 7919-7924 NN denotes water
T13352 7924-7926 , denotes ,
T13353 7926-7941 VBG denotes counterstaining
T13354 7942-7946 IN denotes with
T13355 7947-7954 JJ denotes Nuclear
T13357 7955-7959 JJ denotes Fast
T13356 7960-7963 NN denotes Red
T13358 7964-7965 -LRB- denotes (
T13359 7965-7971 NNP denotes Vector
T13360 7972-7976 NNP denotes Labs
T13361 7976-7977 -RRB- denotes )
T13362 7977-7979 , denotes ,
T13363 7979-7986 VBG denotes rinsing
T13364 7987-7991 IN denotes with
T13365 7992-7997 NN denotes water
T13366 7998-8003 RB denotes again
T13367 8003-8005 , denotes ,
T13368 8005-8008 CC denotes and
T13369 8009-8013 RB denotes then
T13370 8014-8022 VBG denotes mounting
T13371 8023-8025 IN denotes in
T13372 8026-8035 NN denotes Aquamount
T13373 8036-8037 -LRB- denotes (
T13375 8037-8043 NNP denotes Lerner
T13374 8044-8048 NNP denotes Labs
T13376 8048-8050 , denotes ,
T13377 8050-8060 NNP denotes Pittsburgh
T13378 8060-8062 , denotes ,
T13379 8062-8074 NNP denotes Pennsylvania
T13380 8074-8076 , denotes ,
T13381 8076-8082 NNP denotes United
T13382 8083-8089 NNP denotes States
T13383 8089-8090 -RRB- denotes )
T13384 8090-8091 . denotes .
T13385 8091-8567 sentence denotes RNA in situ hybridization was performed as described (Storm and Kingsley 1996), with the following modifications: (1) Prior to the acetylation step, sections were incubated with 10–20 μg/ml proteinase K for 30 s to 7 min at room temperature (depending on the developmental stage), followed by refixing in 4% PFA and washing three times in PBS; (2) prehybridization step was skipped, and (3) embryonic tissue sections used a different color development mix (Thut et al. 2001).
T13386 8092-8095 NN denotes RNA
T13388 8096-8098 FW denotes in
T13389 8099-8103 FW denotes situ
T13387 8104-8117 NN denotes hybridization
T13391 8118-8121 VBD denotes was
T13390 8122-8131 VBN denotes performed
T13392 8132-8134 IN denotes as
T13393 8135-8144 VBN denotes described
T13394 8145-8146 -LRB- denotes (
T13395 8146-8151 NNP denotes Storm
T13396 8152-8155 CC denotes and
T13397 8156-8164 NNP denotes Kingsley
T13398 8165-8169 CD denotes 1996
T13399 8169-8170 -RRB- denotes )
T13400 8170-8172 , denotes ,
T13401 8172-8176 IN denotes with
T13402 8177-8180 DT denotes the
T13404 8181-8190 VBG denotes following
T13403 8191-8204 NNS denotes modifications
T13405 8204-8206 : denotes :
T13406 8206-8207 -LRB- denotes (
T13407 8207-8208 LS denotes 1
T13409 8208-8209 -RRB- denotes )
T13410 8210-8215 JJ denotes Prior
T13411 8216-8218 IN denotes to
T13412 8219-8222 DT denotes the
T13414 8223-8234 NN denotes acetylation
T13413 8235-8239 NN denotes step
T13415 8239-8241 , denotes ,
T13416 8241-8249 NNS denotes sections
T13417 8250-8254 VBD denotes were
T13408 8255-8264 VBN denotes incubated
T13418 8265-8269 IN denotes with
T13419 8270-8272 CD denotes 10
T13421 8272-8273 HYPH denotes
T13420 8273-8275 CD denotes 20
T13422 8276-8278 NNS denotes μg
T13424 8278-8279 SYM denotes /
T13425 8279-8281 NN denotes ml
T13426 8282-8292 NN denotes proteinase
T13423 8293-8294 NN denotes K
T13427 8295-8298 IN denotes for
T13428 8299-8301 CD denotes 30
T13429 8302-8303 NN denotes s
T13430 8304-8306 IN denotes to
T13431 8307-8308 CD denotes 7
T13432 8309-8312 NN denotes min
T13433 8313-8315 IN denotes at
T13434 8316-8320 NN denotes room
T13435 8321-8332 NN denotes temperature
T13436 8333-8334 -LRB- denotes (
T13437 8334-8343 VBG denotes depending
T13438 8344-8346 IN denotes on
T13439 8347-8350 DT denotes the
T13441 8351-8364 JJ denotes developmental
T13440 8365-8370 NN denotes stage
T13442 8370-8371 -RRB- denotes )
T13443 8371-8373 , denotes ,
T13444 8373-8381 VBN denotes followed
T13445 8382-8384 IN denotes by
T13446 8385-8393 VBG denotes refixing
T13447 8394-8396 IN denotes in
T13448 8397-8398 CD denotes 4
T13449 8398-8399 NN denotes %
T13450 8400-8403 NN denotes PFA
T13451 8404-8407 CC denotes and
T13452 8408-8415 VBG denotes washing
T13453 8416-8421 CD denotes three
T13454 8422-8427 NNS denotes times
T13455 8428-8430 IN denotes in
T13456 8431-8434 NN denotes PBS
T13457 8434-8435 : denotes ;
T13458 8436-8437 -LRB- denotes (
T13459 8437-8438 LS denotes 2
T13461 8438-8439 -RRB- denotes )
T13462 8440-8456 NN denotes prehybridization
T13463 8457-8461 NN denotes step
T13464 8462-8465 VBD denotes was
T13460 8466-8473 VBN denotes skipped
T13465 8473-8475 , denotes ,
T13466 8475-8478 CC denotes and
T13467 8479-8480 -LRB- denotes (
T13468 8480-8481 LS denotes 3
T13470 8481-8482 -RRB- denotes )
T13471 8483-8492 JJ denotes embryonic
T13472 8493-8499 NN denotes tissue
T13473 8500-8508 NNS denotes sections
T13469 8509-8513 VBD denotes used
T13474 8514-8515 DT denotes a
T13476 8516-8525 JJ denotes different
T13477 8526-8531 NN denotes color
T13478 8532-8543 NN denotes development
T13475 8544-8547 NN denotes mix
T13479 8548-8549 -LRB- denotes (
T13480 8549-8553 NNP denotes Thut
T13481 8554-8556 FW denotes et
T13482 8557-8560 FW denotes al.
T13483 8561-8565 CD denotes 2001
T13484 8565-8566 -RRB- denotes )
T13485 8566-8567 . denotes .
T13486 8567-8830 sentence denotes Probes for the following genes have been published previously: Bmpr1a (Mishina et al. 1995), Col2a1 (Metsaranta et al. 1991), Col10a1 (Apte et al. 1992), Gdf5 (Storm and Kingsley 1996), Osteocalcin (Celeste et al. 1986), and Sox5 and Sox6 (Lefebvre et al. 1998).
T13487 8568-8574 NNS denotes Probes
T13489 8575-8578 IN denotes for
T13490 8579-8582 DT denotes the
T13492 8583-8592 VBG denotes following
T13491 8593-8598 NNS denotes genes
T13493 8599-8603 VBP denotes have
T13494 8604-8608 VBN denotes been
T13488 8609-8618 VBN denotes published
T13495 8619-8629 RB denotes previously
T13496 8629-8631 : denotes :
T13497 8631-8637 NN denotes Bmpr1a
T13498 8638-8639 -LRB- denotes (
T13499 8639-8646 NNP denotes Mishina
T13500 8647-8649 FW denotes et
T13501 8650-8653 FW denotes al.
T13502 8654-8658 CD denotes 1995
T13503 8658-8659 -RRB- denotes )
T13504 8659-8661 , denotes ,
T13505 8661-8667 NN denotes Col2a1
T13506 8668-8669 -LRB- denotes (
T13507 8669-8679 NNP denotes Metsaranta
T13508 8680-8682 FW denotes et
T13509 8683-8686 FW denotes al.
T13510 8687-8691 CD denotes 1991
T13511 8691-8692 -RRB- denotes )
T13512 8692-8694 , denotes ,
T13513 8694-8701 NN denotes Col10a1
T13514 8702-8703 -LRB- denotes (
T13515 8703-8707 NNP denotes Apte
T13516 8708-8710 FW denotes et
T13517 8711-8714 FW denotes al.
T13518 8715-8719 CD denotes 1992
T13519 8719-8720 -RRB- denotes )
T13520 8720-8722 , denotes ,
T13521 8722-8726 NN denotes Gdf5
T13522 8727-8728 -LRB- denotes (
T13523 8728-8733 NNP denotes Storm
T13524 8734-8737 CC denotes and
T13525 8738-8746 NNP denotes Kingsley
T13526 8747-8751 CD denotes 1996
T13527 8751-8752 -RRB- denotes )
T13528 8752-8754 , denotes ,
T13529 8754-8765 NN denotes Osteocalcin
T13530 8766-8767 -LRB- denotes (
T13531 8767-8774 NNP denotes Celeste
T13532 8775-8777 FW denotes et
T13533 8778-8781 FW denotes al.
T13534 8782-8786 CD denotes 1986
T13535 8786-8787 -RRB- denotes )
T13536 8787-8789 , denotes ,
T13537 8789-8792 CC denotes and
T13538 8793-8797 NN denotes Sox5
T13539 8798-8801 CC denotes and
T13540 8802-8806 NN denotes Sox6
T13541 8807-8808 -LRB- denotes (
T13542 8808-8816 NNP denotes Lefebvre
T13543 8817-8819 FW denotes et
T13544 8820-8823 FW denotes al.
T13545 8824-8828 CD denotes 1998
T13546 8828-8829 -RRB- denotes )
T13547 8829-8830 . denotes .
T13548 8830-9178 sentence denotes The following probe templates were gifts: Agg, Dr. Vicki Rosen, Genetics Institute; Bmp2 and Bmp4, Arend Sidow, Stanford University; Col1a1, Bjorn Olsen, Harvard Medical School; Bmpr1b, Col3a1, and Mat4 probes were made from ESTs with IMAGE clone numbers 5056341, 478480, and 406027, respectively (Invitrogen, Carlsbad, California, United States).
T13549 8831-8834 DT denotes The
T13551 8835-8844 VBG denotes following
T13552 8845-8850 NN denotes probe
T13550 8851-8860 NNS denotes templates
T13553 8861-8865 VBD denotes were
T13555 8866-8871 NNS denotes gifts
T13556 8871-8873 : denotes :
T13557 8873-8876 NN denotes Agg
T13558 8876-8878 , denotes ,
T13559 8878-8881 NNP denotes Dr.
T13561 8882-8887 NNP denotes Vicki
T13560 8888-8893 NNP denotes Rosen
T13562 8893-8895 , denotes ,
T13563 8895-8903 NNP denotes Genetics
T13564 8904-8913 NNP denotes Institute
T13565 8913-8914 : denotes ;
T13566 8915-8919 NN denotes Bmp2
T13567 8920-8923 CC denotes and
T13568 8924-8928 NN denotes Bmp4
T13569 8928-8930 , denotes ,
T13570 8930-8935 NNP denotes Arend
T13571 8936-8941 NNP denotes Sidow
T13572 8941-8943 , denotes ,
T13573 8943-8951 NNP denotes Stanford
T13574 8952-8962 NNP denotes University
T13575 8962-8963 : denotes ;
T13576 8964-8970 NN denotes Col1a1
T13577 8970-8972 , denotes ,
T13578 8972-8977 NNP denotes Bjorn
T13579 8978-8983 NNP denotes Olsen
T13580 8983-8985 , denotes ,
T13581 8985-8992 NNP denotes Harvard
T13583 8993-9000 NNP denotes Medical
T13582 9001-9007 NNP denotes School
T13584 9007-9008 : denotes ;
T13585 9009-9015 NN denotes Bmpr1b
T13587 9015-9017 , denotes ,
T13588 9017-9023 NN denotes Col3a1
T13589 9023-9025 , denotes ,
T13590 9025-9028 CC denotes and
T13591 9029-9033 NN denotes Mat4
T13586 9034-9040 NNS denotes probes
T13592 9041-9045 VBD denotes were
T13554 9046-9050 VBN denotes made
T13593 9051-9055 IN denotes from
T13594 9056-9060 NNS denotes ESTs
T13595 9061-9065 IN denotes with
T13596 9066-9071 NN denotes IMAGE
T13598 9072-9077 NN denotes clone
T13597 9078-9085 NNS denotes numbers
T13599 9086-9093 CD denotes 5056341
T13600 9093-9095 , denotes ,
T13601 9095-9101 CD denotes 478480
T13602 9101-9103 , denotes ,
T13603 9103-9106 CC denotes and
T13604 9107-9113 CD denotes 406027
T13605 9113-9115 , denotes ,
T13606 9115-9127 RB denotes respectively
T13607 9128-9129 -LRB- denotes (
T13608 9129-9139 NNP denotes Invitrogen
T13609 9139-9141 , denotes ,
T13610 9141-9149 NNP denotes Carlsbad
T13611 9149-9151 , denotes ,
T13612 9151-9161 NNP denotes California
T13613 9161-9163 , denotes ,
T13614 9163-9169 NNP denotes United
T13615 9170-9176 NNP denotes States
T13616 9176-9177 -RRB- denotes )
T13617 9177-9178 . denotes .
T13618 9178-9409 sentence denotes Sections for immunohistochemistry were fixed in 4% PFA, then digested with 942–2,000 U/ml type IV-S bovine hyaluronindase (Sigma, St. Louis, Missouri, United States) in PBS (pH 5) at 37 °C for 30 min to 2 h depending on the stage.
T13619 9179-9187 NNS denotes Sections
T13621 9188-9191 IN denotes for
T13622 9192-9212 NN denotes immunohistochemistry
T13623 9213-9217 VBD denotes were
T13620 9218-9223 VBN denotes fixed
T13624 9224-9226 IN denotes in
T13625 9227-9228 CD denotes 4
T13626 9228-9229 NN denotes %
T13627 9230-9233 NN denotes PFA
T13628 9233-9235 , denotes ,
T13629 9235-9239 RB denotes then
T13630 9240-9248 VBN denotes digested
T13631 9249-9253 IN denotes with
T13632 9254-9257 CD denotes 942
T13634 9257-9258 SYM denotes
T13633 9258-9263 CD denotes 2,000
T13635 9264-9265 NNS denotes U
T13637 9265-9266 SYM denotes /
T13638 9266-9268 NN denotes ml
T13639 9269-9273 NN denotes type
T13641 9274-9276 CD denotes IV
T13642 9276-9277 HYPH denotes -
T13640 9277-9278 NN denotes S
T13643 9279-9285 JJ denotes bovine
T13636 9286-9300 NN denotes hyaluronindase
T13644 9301-9302 -LRB- denotes (
T13645 9302-9307 NNP denotes Sigma
T13646 9307-9309 , denotes ,
T13647 9309-9312 NNP denotes St.
T13648 9313-9318 NNP denotes Louis
T13649 9318-9320 , denotes ,
T13650 9320-9328 NNP denotes Missouri
T13651 9328-9330 , denotes ,
T13652 9330-9336 NNP denotes United
T13653 9337-9343 NNP denotes States
T13654 9343-9344 -RRB- denotes )
T13655 9345-9347 IN denotes in
T13656 9348-9351 NNS denotes PBS
T13657 9352-9353 -LRB- denotes (
T13658 9353-9355 NN denotes pH
T13659 9356-9357 CD denotes 5
T13660 9357-9358 -RRB- denotes )
T13661 9359-9361 IN denotes at
T13662 9362-9364 CD denotes 37
T13663 9365-9367 NN denotes °C
T13664 9368-9371 IN denotes for
T13665 9372-9374 CD denotes 30
T13666 9375-9378 NN denotes min
T13667 9379-9381 IN denotes to
T13668 9382-9383 CD denotes 2
T13669 9384-9385 NN denotes h
T13670 9386-9395 VBG denotes depending
T13671 9396-9398 IN denotes on
T13672 9399-9402 DT denotes the
T13673 9403-9408 NN denotes stage
T13674 9408-9409 . denotes .
T13675 9409-9707 sentence denotes Slides were then washed in PBS, treated with 0.3% hydrogen peroxide in 100% methanol for 30 min, washed, blocked with PBS + 0.05% Tween20 + 5% goat or fetal bovine serum, washed again, and incubated with primary antibodies in PBS + 0.05% Tween 20 + 1% goat or fetal bovine serum overnight at 4 °C.
T13676 9410-9416 NNS denotes Slides
T13678 9417-9421 VBD denotes were
T13679 9422-9426 RB denotes then
T13677 9427-9433 VBN denotes washed
T13680 9434-9436 IN denotes in
T13681 9437-9440 NN denotes PBS
T13682 9440-9442 , denotes ,
T13683 9442-9449 VBN denotes treated
T13684 9450-9454 IN denotes with
T13685 9455-9458 CD denotes 0.3
T13686 9458-9459 NN denotes %
T13688 9460-9468 NN denotes hydrogen
T13687 9469-9477 NN denotes peroxide
T13689 9478-9480 IN denotes in
T13690 9481-9484 CD denotes 100
T13691 9484-9485 NN denotes %
T13692 9486-9494 NN denotes methanol
T13693 9495-9498 IN denotes for
T13694 9499-9501 CD denotes 30
T13695 9502-9505 NN denotes min
T13696 9505-9507 , denotes ,
T13697 9507-9513 VBN denotes washed
T13698 9513-9515 , denotes ,
T13699 9515-9522 VBN denotes blocked
T13700 9523-9527 IN denotes with
T13701 9528-9531 NN denotes PBS
T13702 9532-9533 SYM denotes +
T13703 9534-9538 CD denotes 0.05
T13704 9538-9539 NN denotes %
T13705 9540-9547 NN denotes Tween20
T13706 9548-9549 SYM denotes +
T13707 9550-9551 CD denotes 5
T13708 9551-9552 NN denotes %
T13710 9553-9557 NN denotes goat
T13711 9558-9560 CC denotes or
T13712 9561-9566 JJ denotes fetal
T13713 9567-9573 JJ denotes bovine
T13709 9574-9579 NN denotes serum
T13714 9579-9581 , denotes ,
T13715 9581-9587 VBD denotes washed
T13716 9588-9593 RB denotes again
T13717 9593-9595 , denotes ,
T13718 9595-9598 CC denotes and
T13719 9599-9608 VBN denotes incubated
T13720 9609-9613 IN denotes with
T13721 9614-9621 JJ denotes primary
T13722 9622-9632 NNS denotes antibodies
T13723 9633-9635 IN denotes in
T13724 9636-9639 NN denotes PBS
T13725 9640-9641 SYM denotes +
T13726 9642-9646 CD denotes 0.05
T13727 9646-9647 NN denotes %
T13728 9648-9653 NN denotes Tween
T13729 9654-9656 CD denotes 20
T13730 9657-9658 SYM denotes +
T13731 9659-9660 CD denotes 1
T13732 9660-9661 NN denotes %
T13734 9662-9666 NN denotes goat
T13735 9667-9669 CC denotes or
T13736 9670-9675 JJ denotes fetal
T13737 9676-9682 JJ denotes bovine
T13733 9683-9688 NN denotes serum
T13738 9689-9698 RB denotes overnight
T13739 9699-9701 IN denotes at
T13740 9702-9703 CD denotes 4
T13741 9704-9706 NN denotes °C
T13742 9706-9707 . denotes .
T13743 9707-9873 sentence denotes Biotin-labeled secondary antibodies (Vector Labs) were tagged with HRP using the Vectastain Elite ABC kit (Vector Labs) followed by detection with DAB (Vector Labs).
T13744 9708-9714 NN denotes Biotin
T13746 9714-9715 HYPH denotes -
T13745 9715-9722 VBN denotes labeled
T13748 9723-9732 JJ denotes secondary
T13747 9733-9743 NNS denotes antibodies
T13750 9744-9745 -LRB- denotes (
T13752 9745-9751 NN denotes Vector
T13751 9752-9756 NNS denotes Labs
T13753 9756-9757 -RRB- denotes )
T13754 9758-9762 VBD denotes were
T13749 9763-9769 VBN denotes tagged
T13755 9770-9774 IN denotes with
T13756 9775-9778 NN denotes HRP
T13757 9779-9784 VBG denotes using
T13758 9785-9788 DT denotes the
T13760 9789-9799 NNP denotes Vectastain
T13761 9800-9805 NNP denotes Elite
T13762 9806-9809 NNP denotes ABC
T13759 9810-9813 NN denotes kit
T13763 9814-9815 -LRB- denotes (
T13765 9815-9821 NNP denotes Vector
T13764 9822-9826 NNPS denotes Labs
T13766 9826-9827 -RRB- denotes )
T13767 9828-9836 VBN denotes followed
T13768 9837-9839 IN denotes by
T13769 9840-9849 NN denotes detection
T13770 9850-9854 IN denotes with
T13771 9855-9858 NN denotes DAB
T13772 9859-9860 -LRB- denotes (
T13774 9860-9866 NNP denotes Vector
T13773 9867-9871 NNPS denotes Labs
T13775 9871-9872 -RRB- denotes )
T13776 9872-9873 . denotes .
T13777 9873-10144 sentence denotes Primary antibodies and dilutions used were: goat anti-mouse MMP13, 1:100 (Chemicon International, Temecula, California, United States); rabbit anti-human SOX9, 1:500 (Morais da Silva et al. 1996); rabbit anti-phosphorylated-SOX9 (SOX9.P), 1:10–1:250 (Huang et al. 2000).
T13778 9874-9881 JJ denotes Primary
T13779 9882-9892 NNS denotes antibodies
T13781 9893-9896 CC denotes and
T13782 9897-9906 NNS denotes dilutions
T13783 9907-9911 VBN denotes used
T13780 9912-9916 VBD denotes were
T13784 9916-9918 : denotes :
T13785 9918-9922 NN denotes goat
T13787 9923-9933 JJ denotes anti-mouse
T13786 9934-9939 NN denotes MMP13
T13788 9939-9941 , denotes ,
T13789 9941-9942 CD denotes 1
T13790 9942-9943 SYM denotes :
T13791 9943-9946 CD denotes 100
T13792 9947-9948 -LRB- denotes (
T13794 9948-9956 NNP denotes Chemicon
T13793 9957-9970 NNP denotes International
T13795 9970-9972 , denotes ,
T13796 9972-9980 NNP denotes Temecula
T13797 9980-9982 , denotes ,
T13798 9982-9992 NNP denotes California
T13799 9992-9994 , denotes ,
T13800 9994-10000 NNP denotes United
T13801 10001-10007 NNP denotes States
T13802 10007-10008 -RRB- denotes )
T13803 10008-10009 : denotes ;
T13804 10010-10016 NN denotes rabbit
T13806 10017-10027 JJ denotes anti-human
T13805 10028-10032 NN denotes SOX9
T13807 10032-10034 , denotes ,
T13808 10034-10035 CD denotes 1
T13809 10035-10036 SYM denotes :
T13810 10036-10039 CD denotes 500
T13811 10040-10041 -LRB- denotes (
T13812 10041-10047 NNP denotes Morais
T13813 10048-10050 NNP denotes da
T13814 10051-10056 NNP denotes Silva
T13815 10057-10059 FW denotes et
T13816 10060-10063 FW denotes al.
T13817 10064-10068 CD denotes 1996
T13818 10068-10069 -RRB- denotes )
T13819 10069-10070 : denotes ;
T13820 10071-10077 NN denotes rabbit
T13822 10078-10097 VBN denotes anti-phosphorylated
T13823 10097-10098 HYPH denotes -
T13821 10098-10102 NN denotes SOX9
T13824 10103-10104 -LRB- denotes (
T13825 10104-10108 NN denotes SOX9
T13827 10108-10109 , denotes .
T13826 10109-10110 NN denotes P
T13828 10110-10111 -RRB- denotes )
T13829 10111-10113 , denotes ,
T13830 10113-10114 CD denotes 1
T13831 10114-10115 SYM denotes :
T13832 10115-10117 CD denotes 10
T13833 10117-10118 SYM denotes
T13834 10118-10119 CD denotes 1
T13835 10119-10120 SYM denotes :
T13836 10120-10123 CD denotes 250
T13837 10124-10125 -LRB- denotes (
T13838 10125-10130 NNP denotes Huang
T13839 10131-10133 FW denotes et
T13840 10134-10137 FW denotes al.
T13841 10138-10142 CD denotes 2000
T13842 10142-10143 -RRB- denotes )
T13843 10143-10144 . denotes .
R7266 T11314 T11313 prep of,Generation
R7267 T11315 T11316 compound Gdf5,Cre
R7268 T11316 T11318 npadvmod Cre,transgenic
R7269 T11317 T11316 punct -,Cre
R7270 T11318 T11319 amod transgenic,mice
R7271 T11319 T11314 pobj mice,of
R7272 T11321 T11322 det A,library
R7273 T11322 T11328 nsubjpass library,screened
R7274 T11323 T11324 compound mouse,BAC
R7275 T11324 T11322 compound BAC,library
R7276 T11325 T11326 compound 129x1,SvJ
R7277 T11326 T11324 compound SvJ,BAC
R7278 T11327 T11326 punct /,SvJ
R7279 T11329 T11330 punct (,Invitrogen
R7280 T11330 T11322 parataxis Invitrogen,library
R7281 T11331 T11330 punct ),Invitrogen
R7282 T11332 T11328 auxpass was,screened
R7283 T11333 T11334 aux to,identify
R7284 T11334 T11328 advcl identify,screened
R7285 T11335 T11336 det a,BAC
R7286 T11336 T11334 dobj BAC,identify
R7287 T11337 T11338 nummod 140,kb
R7288 T11338 T11336 compound kb,BAC
R7289 T11339 T11338 punct -,kb
R7290 T11340 T11336 prep from,BAC
R7291 T11341 T11342 det the,locus
R7292 T11342 T11340 pobj locus,from
R7293 T11343 T11342 compound Gdf5,locus
R7294 T11344 T11328 punct .,screened
R7295 T11346 T11347 det This,BAC
R7296 T11347 T11348 nsubjpass BAC,modified
R7297 T11349 T11348 auxpass was,modified
R7298 T11350 T11348 advcl using,modified
R7299 T11351 T11352 det a,system
R7300 T11352 T11350 dobj system,using
R7301 T11353 T11354 amod homologous,recombination
R7302 T11354 T11352 compound recombination,system
R7303 T11355 T11350 prep in,using
R7304 T11356 T11357 compound E.,coli
R7305 T11357 T11355 pobj coli,in
R7306 T11358 T11359 punct (,Yang
R7307 T11359 T11350 meta Yang,using
R7308 T11360 T11359 nmod et,Yang
R7309 T11361 T11359 nmod al.,Yang
R7310 T11362 T11359 nummod 1997,Yang
R7311 T11363 T11359 punct ),Yang
R7312 T11364 T11365 aux to,place
R7313 T11365 T11350 advcl place,using
R7314 T11366 T11367 amod nuclear,localized
R7315 T11367 T11369 amod localized,recombinase
R7316 T11368 T11367 punct -,localized
R7317 T11369 T11365 dobj recombinase,place
R7318 T11370 T11369 compound Cre,recombinase
R7319 T11371 T11369 punct (,recombinase
R7320 T11372 T11369 prep from,recombinase
R7321 T11373 T11374 compound plasmid,pML78
R7322 T11374 T11372 pobj pML78,from
R7323 T11375 T11376 punct ", ",gift
R7324 T11376 T11374 parataxis gift,pML78
R7325 T11377 T11376 prep of,gift
R7326 T11378 T11379 compound Gail,Martin
R7327 T11379 T11377 pobj Martin,of
R7328 T11380 T11369 punct ),recombinase
R7329 T11381 T11369 acl followed,recombinase
R7330 T11382 T11381 agent by,followed
R7331 T11383 T11384 compound IRES,hPLAP
R7332 T11384 T11382 pobj hPLAP,by
R7333 T11385 T11384 punct -,hPLAP
R7334 T11386 T11384 punct (,hPLAP
R7335 T11387 T11384 prep from,hPLAP
R7336 T11388 T11387 pobj plasmid,from
R7337 T11389 T11388 nummod 1726,plasmid
R7338 T11390 T11388 punct ", ",plasmid
R7339 T11391 T11388 parataxis gift,plasmid
R7340 T11392 T11391 prep of,gift
R7341 T11393 T11394 compound Oliver,Bogler
R7342 T11394 T11392 pobj Bogler,of
R7343 T11395 T11384 punct ),hPLAP
R7344 T11396 T11397 advmod directly,behind
R7345 T11397 T11384 prep behind,hPLAP
R7346 T11398 T11399 det the,site
R7347 T11399 T11397 pobj site,behind
R7348 T11400 T11399 compound ATG,site
R7349 T11401 T11399 compound start,site
R7350 T11402 T11399 prep of,site
R7351 T11403 T11402 pobj Gdf5,of
R7352 T11404 T11348 punct .,modified
R7353 T11406 T11407 prep In,removed
R7354 T11408 T11409 det the,process
R7355 T11409 T11406 pobj process,In
R7356 T11410 T11407 punct ", ",removed
R7357 T11411 T11412 nummod 583,bp
R7358 T11412 T11407 nsubjpass bp,removed
R7359 T11413 T11412 prep of,bp
R7360 T11414 T11415 det the,exon
R7361 T11415 T11413 pobj exon,of
R7362 T11416 T11415 amod first,exon
R7363 T11417 T11415 prep of,exon
R7364 T11418 T11417 pobj Gdf5,of
R7365 T11419 T11407 auxpass was,removed
R7366 T11420 T11407 cc and,removed
R7367 T11421 T11422 det no,protein
R7368 T11422 T11425 nsubjpass protein,predicted
R7369 T11423 T11422 amod functional,protein
R7370 T11424 T11422 compound GDF5,protein
R7371 T11425 T11407 conj predicted,removed
R7372 T11426 T11425 auxpass is,predicted
R7373 T11427 T11428 aux to,produced
R7374 T11428 T11425 xcomp produced,predicted
R7375 T11429 T11428 auxpass be,produced
R7376 T11430 T11425 punct .,predicted
R7377 T11432 T11433 det The,arm
R7378 T11433 T11437 nsubjpass arm,subcloned
R7379 T11434 T11433 nummod 5,arm
R7380 T11435 T11434 punct ′,5
R7381 T11436 T11433 compound homology,arm
R7382 T11438 T11437 auxpass was,subcloned
R7383 T11439 T11437 prep from,subcloned
R7384 T11440 T11441 det a,product
R7385 T11441 T11439 pobj product,from
R7386 T11442 T11441 compound PCR,product
R7387 T11443 T11441 acl tailed,product
R7388 T11444 T11443 prep with,tailed
R7389 T11445 T11446 nmod XhoI,sites
R7390 T11446 T11444 pobj sites,with
R7391 T11447 T11445 cc and,XhoI
R7392 T11448 T11445 conj Bsp120I,XhoI
R7393 T11449 T11446 compound restriction,sites
R7394 T11450 T11451 dep that,contains
R7395 T11451 T11441 relcl contains,product
R7396 T11452 T11453 nummod 781,bp
R7397 T11453 T11451 dobj bp,contains
R7398 T11454 T11453 prep of,bp
R7399 T11455 T11456 nummod 5,sequence
R7400 T11456 T11454 pobj sequence,of
R7401 T11457 T11455 punct ′,5
R7402 T11458 T11456 amod genomic,sequence
R7403 T11459 T11456 compound Gdf5,sequence
R7404 T11460 T11456 acl ending,sequence
R7405 T11461 T11460 prep at,ending
R7406 T11462 T11463 det the,site
R7407 T11463 T11461 pobj site,at
R7408 T11464 T11465 compound ATG,translation
R7409 T11465 T11463 compound translation,site
R7410 T11466 T11463 compound start,site
R7411 T11467 T11468 punct (,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7412 T11468 T11460 parataxis GTTTGGGCCCATCCTCTGGCCAGCCGCTG,ending
R7413 T11469 T11470 amod forward,primer
R7414 T11470 T11471 dep primer,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7415 T11471 T11468 dep CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7416 T11472 T11471 nummod 5,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7417 T11473 T11472 punct ′,5
R7418 T11474 T11471 punct -,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7419 T11475 T11471 punct -,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7420 T11476 T11471 nummod 3,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7421 T11477 T11471 punct ′,CTGTCTCGAGATGAGGTGGAGGTGAAGACCCC
R7422 T11478 T11468 punct ;,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7423 T11479 T11468 amod reverse,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7424 T11480 T11468 nummod 5,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7425 T11481 T11480 punct ′,5
R7426 T11482 T11468 punct -,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7427 T11483 T11468 punct -,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7428 T11484 T11468 nummod 3,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7429 T11485 T11468 punct ′,GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7430 T11486 T11468 punct ),GTTTGGGCCCATCCTCTGGCCAGCCGCTG
R7431 T11487 T11437 punct .,subcloned
R7432 T11489 T11490 nsubjpass Cre,subcloned
R7433 T11491 T11490 auxpass was,subcloned
R7434 T11492 T11490 prep from,subcloned
R7435 T11493 T11494 det a,fragment
R7436 T11494 T11492 pobj fragment,from
R7437 T11495 T11496 nummod 1.1,kb
R7438 T11496 T11494 compound kb,fragment
R7439 T11497 T11496 punct -,kb
R7440 T11498 T11499 compound Bsp120I,EcoRI
R7441 T11499 T11494 compound EcoRI,fragment
R7442 T11500 T11499 punct /,EcoRI
R7443 T11501 T11494 prep of,fragment
R7444 T11502 T11501 pobj pML78,of
R7445 T11503 T11490 punct .,subcloned
R7446 T11505 T11506 compound IRES,hPLAP
R7447 T11506 T11507 nsubjpass hPLAP,subcloned
R7448 T11508 T11507 auxpass was,subcloned
R7449 T11509 T11507 prep from,subcloned
R7450 T11510 T11511 det a,product
R7451 T11511 T11509 pobj product,from
R7452 T11512 T11513 nummod 2.1,kb
R7453 T11513 T11511 compound kb,product
R7454 T11514 T11513 punct -,kb
R7455 T11515 T11511 compound PCR,product
R7456 T11516 T11511 acl tailed,product
R7457 T11517 T11516 prep with,tailed
R7458 T11518 T11519 nmod EcoRI,sites
R7459 T11519 T11517 pobj sites,with
R7460 T11520 T11518 cc and,EcoRI
R7461 T11521 T11518 conj SpeI,EcoRI
R7462 T11522 T11523 dep that,contains
R7463 T11523 T11511 relcl contains,product
R7464 T11524 T11525 det the,site
R7465 T11525 T11523 dobj site,contains
R7466 T11526 T11527 compound hPLAP,translation
R7467 T11527 T11525 compound translation,site
R7468 T11528 T11525 compound stop,site
R7469 T11529 T11530 punct (,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7470 T11530 T11525 parataxis AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG,site
R7471 T11531 T11532 amod forward,primer
R7472 T11532 T11533 dep primer,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7473 T11533 T11530 dep ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7474 T11534 T11533 nummod 5,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7475 T11535 T11534 punct ′,5
R7476 T11536 T11533 punct -,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7477 T11537 T11533 punct -,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7478 T11538 T11533 nummod 3,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7479 T11539 T11533 punct ′,ATCTCTCGAGGAATTCTCCACCATATTGCCGTCTTTTG
R7480 T11540 T11530 punct ;,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7481 T11541 T11530 amod reverse,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7482 T11542 T11530 nummod 5,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7483 T11543 T11542 punct ′,5
R7484 T11544 T11530 punct -,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7485 T11545 T11530 punct -,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7486 T11546 T11530 nummod 3,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7487 T11547 T11530 punct ′,AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7488 T11548 T11530 punct ),AGAACTCGAGACTAGTCGGGACACTCAGGGAGTAGTGG
R7489 T11549 T11507 punct .,subcloned
R7490 T11551 T11552 det The,arm
R7491 T11552 T11556 nsubjpass arm,subcloned
R7492 T11553 T11552 nummod 3,arm
R7493 T11554 T11553 punct ′,3
R7494 T11555 T11552 compound homology,arm
R7495 T11557 T11556 auxpass was,subcloned
R7496 T11558 T11556 prep from,subcloned
R7497 T11559 T11560 det a,product
R7498 T11560 T11558 pobj product,from
R7499 T11561 T11562 nummod 0.8,kb
R7500 T11562 T11560 compound kb,product
R7501 T11563 T11562 punct -,kb
R7502 T11564 T11560 compound PCR,product
R7503 T11565 T11560 acl amplified,product
R7504 T11566 T11565 prep from,amplified
R7505 T11567 T11568 det a,subclone
R7506 T11568 T11566 pobj subclone,from
R7507 T11569 T11570 nummod 0.9,kb
R7508 T11570 T11568 nmod kb,subclone
R7509 T11571 T11570 punct -,kb
R7510 T11572 T11573 nmod XhoI,Gdf5
R7511 T11573 T11568 nmod Gdf5,subclone
R7512 T11574 T11568 amod genomic,subclone
R7513 T11575 T11568 acl containing,subclone
R7514 T11576 T11575 dobj part,containing
R7515 T11577 T11576 prep of,part
R7516 T11578 T11579 det the,exon
R7517 T11579 T11577 pobj exon,of
R7518 T11580 T11579 amod first,exon
R7519 T11581 T11579 cc and,exon
R7520 T11582 T11583 amod downstream,intron
R7521 T11583 T11579 conj intron,exon
R7522 T11584 T11556 punct .,subcloned
R7523 T11586 T11587 det The,primer
R7524 T11587 T11589 nsubj primer,contains
R7525 T11588 T11587 amod forward,primer
R7526 T11589 T11590 ccomp contains,is
R7527 T11591 T11592 det the,end
R7528 T11592 T11589 dobj end,contains
R7529 T11593 T11592 nummod 3,end
R7530 T11594 T11593 punct ′,3
R7531 T11595 T11592 prep of,end
R7532 T11596 T11597 det the,exon
R7533 T11597 T11595 pobj exon,of
R7534 T11598 T11597 amod first,exon
R7535 T11599 T11589 cc and,contains
R7536 T11600 T11601 auxpass is,tailed
R7537 T11601 T11589 conj tailed,contains
R7538 T11602 T11601 prep with,tailed
R7539 T11603 T11604 det a,site
R7540 T11604 T11602 pobj site,with
R7541 T11605 T11604 compound SpeI,site
R7542 T11606 T11590 punct ;,is
R7543 T11607 T11608 det the,primer
R7544 T11608 T11590 nsubj primer,is
R7545 T11609 T11608 amod reverse,primer
R7546 T11610 T11590 prep from,is
R7547 T11611 T11612 det the,promoter
R7548 T11612 T11610 pobj promoter,from
R7549 T11613 T11612 compound T7,promoter
R7550 T11614 T11612 prep of,promoter
R7551 T11615 T11616 det the,vector
R7552 T11616 T11614 pobj vector,of
R7553 T11617 T11616 acl containing,vector
R7554 T11618 T11619 det the,subclone
R7555 T11619 T11617 dobj subclone,containing
R7556 T11620 T11621 nummod 0.9,kb
R7557 T11621 T11619 compound kb,subclone
R7558 T11622 T11621 punct -,kb
R7559 T11623 T11590 cc and,is
R7560 T11624 T11590 conj flanks,is
R7561 T11625 T11626 det the,site
R7562 T11626 T11624 dobj site,flanks
R7563 T11627 T11628 amod intronic,XhoI
R7564 T11628 T11626 compound XhoI,site
R7565 T11629 T11630 punct (,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7566 T11630 T11626 parataxis GATTTCTAGAGTAATACGACTCACTATAGGGC,site
R7567 T11631 T11632 amod forward,primer
R7568 T11632 T11633 dep primer,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7569 T11633 T11630 dep CTAAACTAGTCACCAGCTTTATTGACAAAGG,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7570 T11634 T11633 nummod 5,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7571 T11635 T11634 punct ′,5
R7572 T11636 T11633 punct -,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7573 T11637 T11633 punct -,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7574 T11638 T11633 nummod 3,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7575 T11639 T11633 punct ′,CTAAACTAGTCACCAGCTTTATTGACAAAGG
R7576 T11640 T11630 punct ;,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7577 T11641 T11630 amod reverse,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7578 T11642 T11630 nummod 5,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7579 T11643 T11642 punct ′,5
R7580 T11644 T11630 punct -,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7581 T11645 T11630 punct -,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7582 T11646 T11630 nummod 3,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7583 T11647 T11630 punct ′,GATTTCTAGAGTAATACGACTCACTATAGGGC
R7584 T11648 T11630 punct ),GATTTCTAGAGTAATACGACTCACTATAGGGC
R7585 T11649 T11590 punct .,is
R7586 T11651 T11652 det The,construct
R7587 T11652 T11654 nsubjpass construct,built
R7588 T11653 T11652 compound targeting,construct
R7589 T11655 T11654 auxpass was,built
R7590 T11656 T11654 cc and,built
R7591 T11657 T11654 conj verified,built
R7592 T11658 T11657 prep in,verified
R7593 T11659 T11658 pobj pBSSK,in
R7594 T11660 T11661 punct (,Stratagene
R7595 T11661 T11659 parataxis Stratagene,pBSSK
R7596 T11662 T11661 punct ", ",Stratagene
R7597 T11663 T11664 compound La,Jolla
R7598 T11664 T11661 npadvmod Jolla,Stratagene
R7599 T11665 T11661 punct ", ",Stratagene
R7600 T11666 T11661 npadvmod California,Stratagene
R7601 T11667 T11661 punct ", ",Stratagene
R7602 T11668 T11669 compound United,States
R7603 T11669 T11661 npadvmod States,Stratagene
R7604 T11670 T11661 punct ),Stratagene
R7605 T11671 T11654 punct ", ",built
R7606 T11672 T11673 advmod then,digested
R7607 T11673 T11654 conj digested,built
R7608 T11674 T11673 prep with,digested
R7609 T11675 T11674 pobj XhoI,with
R7610 T11676 T11673 cc and,digested
R7611 T11677 T11673 conj subcloned,digested
R7612 T11678 T11677 prep into,subcloned
R7613 T11679 T11678 pobj pSV1,into
R7614 T11680 T11679 punct ", ",pSV1
R7615 T11681 T11682 det the,vector
R7616 T11682 T11679 appos vector,pSV1
R7617 T11683 T11682 acl used,vector
R7618 T11684 T11683 prep for,used
R7619 T11685 T11686 amod homologous,recombination
R7620 T11686 T11684 pobj recombination,for
R7621 T11687 T11688 punct (,Yang
R7622 T11688 T11683 meta Yang,used
R7623 T11689 T11688 nmod et,Yang
R7624 T11690 T11688 nmod al.,Yang
R7625 T11691 T11688 nummod 1997,Yang
R7626 T11692 T11688 punct ),Yang
R7627 T11693 T11654 punct .,built
R7628 T11695 T11696 nmod Southern,blotting
R7629 T11696 T11697 nmod blotting,analysis
R7630 T11697 T11704 nsubj analysis,confirmed
R7631 T11698 T11696 punct ", ",blotting
R7632 T11699 T11696 conj PCR,blotting
R7633 T11700 T11699 punct ", ",PCR
R7634 T11701 T11699 cc and,PCR
R7635 T11702 T11703 compound DNA,sequence
R7636 T11703 T11699 conj sequence,PCR
R7637 T11705 T11706 det the,construct
R7638 T11706 T11709 nsubjpass construct,made
R7639 T11707 T11706 amod appropriate,construct
R7640 T11708 T11706 compound targeting,construct
R7641 T11709 T11704 advcl made,confirmed
R7642 T11710 T11706 cc and,construct
R7643 T11711 T11712 compound BAC,modifications
R7644 T11712 T11706 conj modifications,construct
R7645 T11713 T11709 auxpass were,made
R7646 T11714 T11715 punct (,data
R7647 T11715 T11709 meta data,made
R7648 T11716 T11715 amod unpublished,data
R7649 T11717 T11715 punct ),data
R7650 T11718 T11704 punct .,confirmed
R7651 T11720 T11721 mark Before,injected
R7652 T11721 T11726 advcl injected,removed
R7653 T11722 T11723 det the,BAC
R7654 T11723 T11721 nsubjpass BAC,injected
R7655 T11724 T11723 amod modified,BAC
R7656 T11725 T11721 auxpass was,injected
R7657 T11727 T11728 aux to,produce
R7658 T11728 T11721 advcl produce,injected
R7659 T11729 T11730 amod transgenic,animals
R7660 T11730 T11728 dobj animals,produce
R7661 T11731 T11726 punct ", ",removed
R7662 T11732 T11733 det a,site
R7663 T11733 T11726 nsubjpass site,removed
R7664 T11734 T11733 compound loxP,site
R7665 T11735 T11733 relcl present,site
R7666 T11736 T11735 prep in,present
R7667 T11737 T11738 det the,vector
R7668 T11738 T11736 pobj vector,in
R7669 T11739 T11738 compound BAC,vector
R7670 T11740 T11733 punct ", ",site
R7671 T11741 T11733 appos pBeloBAC11,site
R7672 T11742 T11726 punct ", ",removed
R7673 T11743 T11726 auxpass was,removed
R7674 T11744 T11745 aux to,prevent
R7675 T11745 T11726 advcl prevent,removed
R7676 T11746 T11747 det the,addition
R7677 T11747 T11745 dobj addition,prevent
R7678 T11748 T11747 prep of,addition
R7679 T11749 T11750 amod undesired,sites
R7680 T11750 T11748 pobj sites,of
R7681 T11751 T11750 compound Cre,sites
R7682 T11752 T11750 compound target,sites
R7683 T11753 T11747 prep into,addition
R7684 T11754 T11755 det the,genome
R7685 T11755 T11753 pobj genome,into
R7686 T11756 T11726 punct .,removed
R7687 T11758 T11759 aux To,do
R7688 T11759 T11760 advcl do,prepared
R7689 T11761 T11759 dobj this,do
R7690 T11762 T11760 punct ", ",prepared
R7691 T11763 T11764 compound BAC,DNA
R7692 T11764 T11760 nsubjpass DNA,prepared
R7693 T11765 T11760 auxpass was,prepared
R7694 T11766 T11760 prep by,prepared
R7695 T11767 T11768 compound CsCl,separation
R7696 T11768 T11766 pobj separation,by
R7697 T11769 T11760 punct ", ",prepared
R7698 T11770 T11760 conj digested,prepared
R7699 T11771 T11770 prep with,digested
R7700 T11772 T11771 pobj NotI,with
R7701 T11773 T11774 aux to,free
R7702 T11774 T11770 advcl free,digested
R7703 T11775 T11776 det the,insert
R7704 T11776 T11774 dobj insert,free
R7705 T11777 T11774 prep from,free
R7706 T11778 T11779 det the,vector
R7707 T11779 T11777 pobj vector,from
R7708 T11780 T11770 punct ", ",digested
R7709 T11781 T11770 cc and,digested
R7710 T11782 T11783 dep size,fractionated
R7711 T11783 T11770 conj fractionated,digested
R7712 T11784 T11783 punct -,fractionated
R7713 T11785 T11783 prep over,fractionated
R7714 T11786 T11787 det a,gradient
R7715 T11787 T11785 pobj gradient,over
R7716 T11788 T11787 compound sucrose,gradient
R7717 T11789 T11760 punct .,prepared
R7718 T11791 T11792 nsubjpass Aliquots,run
R7719 T11793 T11791 prep of,Aliquots
R7720 T11794 T11793 pobj fractions,of
R7721 T11795 T11792 auxpass were,run
R7722 T11796 T11792 prep on,run
R7723 T11797 T11798 det a,gel
R7724 T11798 T11796 pobj gel,on
R7725 T11799 T11800 compound pulse,field
R7726 T11800 T11798 compound field,gel
R7727 T11801 T11800 punct -,field
R7728 T11802 T11792 cc and,run
R7729 T11803 T11804 dep Southern,blotted
R7730 T11804 T11792 conj blotted,run
R7731 T11805 T11804 advcl using,blotted
R7732 T11806 T11807 nmod vector,DNA
R7733 T11807 T11805 dobj DNA,using
R7734 T11808 T11806 punct -,vector
R7735 T11809 T11806 amod specific,vector
R7736 T11810 T11805 prep as,using
R7737 T11811 T11812 det a,probe
R7738 T11812 T11810 pobj probe,as
R7739 T11813 T11792 punct .,run
R7740 T11815 T11816 nsubjpass Fractions,dialyzed
R7741 T11817 T11815 acl containing,Fractions
R7742 T11818 T11819 amod unsheared,insert
R7743 T11819 T11817 dobj insert,containing
R7744 T11820 T11819 cc and,insert
R7745 T11821 T11822 advmod almost,DNA
R7746 T11822 T11819 conj DNA,insert
R7747 T11823 T11822 det no,DNA
R7748 T11824 T11822 amod detectable,DNA
R7749 T11825 T11822 compound vector,DNA
R7750 T11826 T11816 auxpass were,dialyzed
R7751 T11827 T11816 prep in,dialyzed
R7752 T11828 T11829 compound microinjection,buffer
R7753 T11829 T11827 pobj buffer,in
R7754 T11830 T11831 punct (,Tris
R7755 T11831 T11829 parataxis Tris,buffer
R7756 T11832 T11833 nummod 10,mM
R7757 T11833 T11831 compound mM,Tris
R7758 T11834 T11831 punct [,Tris
R7759 T11835 T11831 npadvmod pH,Tris
R7760 T11836 T11835 nummod 7.4,pH
R7761 T11837 T11831 punct ],Tris
R7762 T11838 T11831 prep with,Tris
R7763 T11839 T11840 nummod 0.15,mM
R7764 T11840 T11841 compound mM,EDTA
R7765 T11841 T11838 pobj EDTA,with
R7766 T11842 T11841 punct [,EDTA
R7767 T11843 T11841 npadvmod pH,EDTA
R7768 T11844 T11843 nummod 8.0,pH
R7769 T11845 T11841 punct ],EDTA
R7770 T11846 T11831 punct ),Tris
R7771 T11847 T11816 advcl using,dialyzed
R7772 T11848 T11849 nmod Centriprep,concentrators
R7773 T11849 T11847 dobj concentrators,using
R7774 T11850 T11848 punct -,Centriprep
R7775 T11851 T11848 nummod 30,Centriprep
R7776 T11852 T11853 punct (,Millipore
R7777 T11853 T11849 parataxis Millipore,concentrators
R7778 T11854 T11853 punct ", ",Millipore
R7779 T11855 T11853 npadvmod Billerica,Millipore
R7780 T11856 T11853 punct ", ",Millipore
R7781 T11857 T11853 npadvmod Massachusetts,Millipore
R7782 T11858 T11853 punct ", ",Millipore
R7783 T11859 T11860 compound United,States
R7784 T11860 T11853 npadvmod States,Millipore
R7785 T11861 T11853 punct ),Millipore
R7786 T11862 T11816 punct .,dialyzed
R7787 T11864 T11865 det This,DNA
R7788 T11865 T11868 nsubj DNA,adjusted
R7789 T11866 T11865 amod purified,DNA
R7790 T11867 T11865 compound insert,DNA
R7791 T11869 T11868 aux was,adjusted
R7792 T11870 T11868 prep to,adjusted
R7793 T11871 T11872 nummod 1,ng
R7794 T11872 T11870 pobj ng,to
R7795 T11873 T11874 punct /,μl
R7796 T11874 T11872 prep μl,ng
R7797 T11875 T11868 cc and,adjusted
R7798 T11876 T11868 conj injected,adjusted
R7799 T11877 T11876 prep into,injected
R7800 T11878 T11879 det the,pronucleus
R7801 T11879 T11877 pobj pronucleus,into
R7802 T11880 T11879 prep of,pronucleus
R7803 T11881 T11882 amod fertilized,eggs
R7804 T11882 T11880 pobj eggs,of
R7805 T11883 T11882 prep from,eggs
R7806 T11884 T11885 compound FVB,N
R7807 T11885 T11887 compound N,mice
R7808 T11886 T11885 punct /,N
R7809 T11887 T11883 pobj mice,from
R7810 T11888 T11868 agent by,adjusted
R7811 T11889 T11890 det the,Facility
R7812 T11890 T11888 pobj Facility,by
R7813 T11891 T11890 compound Stanford,Facility
R7814 T11892 T11890 compound Transgenic,Facility
R7815 T11893 T11868 punct .,adjusted
R7816 T11895 T11896 amod Transgenic,mice
R7817 T11896 T11898 nsubjpass mice,identified
R7818 T11897 T11896 compound founder,mice
R7819 T11899 T11898 auxpass were,identified
R7820 T11900 T11898 prep by,identified
R7821 T11901 T11900 pobj PCR,by
R7822 T11902 T11901 acl using,PCR
R7823 T11903 T11904 npadvmod Cre,specific
R7824 T11904 T11906 amod specific,GCCTGCATTACCGGTCGATGCAACGA
R7825 T11905 T11904 punct -,specific
R7826 T11906 T11902 dobj GCCTGCATTACCGGTCGATGCAACGA,using
R7827 T11907 T11906 nmod primers,GCCTGCATTACCGGTCGATGCAACGA
R7828 T11908 T11906 nummod 5,GCCTGCATTACCGGTCGATGCAACGA
R7829 T11909 T11908 punct ′,5
R7830 T11910 T11906 punct -,GCCTGCATTACCGGTCGATGCAACGA
R7831 T11911 T11906 punct -,GCCTGCATTACCGGTCGATGCAACGA
R7832 T11912 T11906 nummod 3,GCCTGCATTACCGGTCGATGCAACGA
R7833 T11913 T11906 punct ′,GCCTGCATTACCGGTCGATGCAACGA
R7834 T11914 T11906 cc and,GCCTGCATTACCGGTCGATGCAACGA
R7835 T11915 T11916 nummod 5,GTGGCAGATGGCGCGGCAACACCATT
R7836 T11916 T11906 conj GTGGCAGATGGCGCGGCAACACCATT,GCCTGCATTACCGGTCGATGCAACGA
R7837 T11917 T11915 punct ′,5
R7838 T11918 T11916 punct -,GTGGCAGATGGCGCGGCAACACCATT
R7839 T11919 T11916 punct -,GTGGCAGATGGCGCGGCAACACCATT
R7840 T11920 T11916 nummod 3,GTGGCAGATGGCGCGGCAACACCATT
R7841 T11921 T11916 punct ′,GTGGCAGATGGCGCGGCAACACCATT
R7842 T11922 T11906 punct ", ",GCCTGCATTACCGGTCGATGCAACGA
R7843 T11923 T11924 dep which,amplify
R7844 T11924 T11906 relcl amplify,GCCTGCATTACCGGTCGATGCAACGA
R7845 T11925 T11926 det a,product
R7846 T11926 T11924 dobj product,amplify
R7847 T11927 T11928 nummod 725,bp
R7848 T11928 T11926 compound bp,product
R7849 T11929 T11928 punct -,bp
R7850 T11930 T11898 punct ", ",identified
R7851 T11931 T11898 cc and,identified
R7852 T11932 T11933 auxpass were,assessed
R7853 T11933 T11898 conj assessed,identified
R7854 T11934 T11933 prep for,assessed
R7855 T11935 T11934 pobj absence,for
R7856 T11936 T11935 prep of,absence
R7857 T11937 T11938 compound BAC,vector
R7858 T11938 T11936 pobj vector,of
R7859 T11939 T11933 advcl using,assessed
R7860 T11940 T11941 nmod vector,primers
R7861 T11941 T11939 dobj primers,using
R7862 T11942 T11940 punct -,vector
R7863 T11943 T11940 amod specific,vector
R7864 T11944 T11945 nummod 5,CGGAGTCTGATGCGGTTGCGATG
R7865 T11945 T11941 appos CGGAGTCTGATGCGGTTGCGATG,primers
R7866 T11946 T11944 punct ′,5
R7867 T11947 T11945 punct -,CGGAGTCTGATGCGGTTGCGATG
R7868 T11948 T11945 punct -,CGGAGTCTGATGCGGTTGCGATG
R7869 T11949 T11945 nummod 3,CGGAGTCTGATGCGGTTGCGATG
R7870 T11950 T11945 punct ′,CGGAGTCTGATGCGGTTGCGATG
R7871 T11951 T11945 cc and,CGGAGTCTGATGCGGTTGCGATG
R7872 T11952 T11953 nummod 5,AGTGCTGTTCCCTGGTGCTTCCTC
R7873 T11953 T11945 conj AGTGCTGTTCCCTGGTGCTTCCTC,CGGAGTCTGATGCGGTTGCGATG
R7874 T11954 T11952 punct ′,5
R7875 T11955 T11953 punct -,AGTGCTGTTCCCTGGTGCTTCCTC
R7876 T11956 T11953 punct -,AGTGCTGTTCCCTGGTGCTTCCTC
R7877 T11957 T11953 nummod 3,AGTGCTGTTCCCTGGTGCTTCCTC
R7878 T11958 T11953 punct ′,AGTGCTGTTCCCTGGTGCTTCCTC
R7879 T11959 T11941 punct ", ",primers
R7880 T11960 T11961 dep which,amplify
R7881 T11961 T11941 relcl amplify,primers
R7882 T11962 T11963 det a,product
R7883 T11963 T11961 dobj product,amplify
R7884 T11964 T11965 nummod 465,bp
R7885 T11965 T11963 compound bp,product
R7886 T11966 T11965 punct -,bp
R7887 T11967 T11898 punct .,identified
R7888 T11969 T11970 nummod Three,lines
R7889 T11970 T11971 nsubjpass lines,established
R7890 T11972 T11970 prep of,lines
R7891 T11973 T11974 compound Gdf5,Cre
R7892 T11974 T11976 compound Cre,mice
R7893 T11975 T11974 punct -,Cre
R7894 T11976 T11972 pobj mice,of
R7895 T11977 T11971 auxpass were,established
R7896 T11978 T11971 cc and,established
R7897 T11979 T11971 conj maintained,established
R7898 T11980 T11979 prep on,maintained
R7899 T11981 T11982 det the,background
R7900 T11982 T11980 pobj background,on
R7901 T11983 T11982 compound FVB,background
R7902 T11984 T11971 punct .,established
R7903 T11986 T11987 nsubjpass Matings,used
R7904 T11988 T11986 prep with,Matings
R7905 T11989 T11990 compound R26R,Cre
R7906 T11990 T11991 npadvmod Cre,inducible
R7907 T11991 T11993 amod inducible,mice
R7908 T11992 T11991 punct -,inducible
R7909 T11993 T11988 pobj mice,with
R7910 T11994 T11995 compound LACZ,reporter
R7911 T11995 T11993 compound reporter,mice
R7912 T11996 T11997 punct (,Soriano
R7913 T11997 T11993 meta Soriano,mice
R7914 T11998 T11997 nummod 1999,Soriano
R7915 T11999 T11997 punct ),Soriano
R7916 T12000 T11987 auxpass were,used
R7917 T12001 T12002 aux to,test
R7918 T12002 T11987 advcl test,used
R7919 T12003 T12002 prep for,test
R7920 T12004 T12005 compound Cre,activity
R7921 T12005 T12003 pobj activity,for
R7922 T12006 T11987 punct .,used
R7923 T12008 T12009 nsubjpass Staining,accomplished
R7924 T12010 T12008 prep for,Staining
R7925 T12011 T12010 pobj LACZ,for
R7926 T12012 T12011 cc and,LACZ
R7927 T12013 T12011 conj HPLAP,LACZ
R7928 T12014 T12008 prep on,Staining
R7929 T12015 T12016 amod whole,embryos
R7930 T12016 T12014 pobj embryos,on
R7931 T12017 T12016 cc or,embryos
R7932 T12018 T12016 conj sections,embryos
R7933 T12019 T12018 prep of,sections
R7934 T12020 T12019 pobj embryos,of
R7935 T12021 T12009 auxpass was,accomplished
R7936 T12022 T12009 advcl following,accomplished
R7937 T12023 T12024 amod established,protocols
R7938 T12024 T12022 dobj protocols,following
R7939 T12025 T12026 punct (,Lobe
R7940 T12026 T12009 meta Lobe,accomplished
R7941 T12027 T12026 nmod et,Lobe
R7942 T12028 T12026 nmod al.,Lobe
R7943 T12029 T12026 nummod 1999,Lobe
R7944 T12030 T12026 punct ),Lobe
R7945 T12031 T12009 punct .,accomplished
R7946 T12033 T12034 det The,substrate
R7947 T12034 T12037 nsubj substrate,is
R7948 T12035 T12036 amod red,LACZ
R7949 T12036 T12034 compound LACZ,substrate
R7950 T12038 T12039 punct (,see
R7951 T12039 T12034 parataxis see,substrate
R7952 T12040 T12039 dobj Figure,see
R7953 T12041 T12040 nummod 1E,Figure
R7954 T12042 T12039 punct ),see
R7955 T12043 T12044 nummod 6,galactopyranoside
R7956 T12044 T12037 attr galactopyranoside,is
R7957 T12045 T12044 punct -,galactopyranoside
R7958 T12046 T12044 nmod chloro,galactopyranoside
R7959 T12047 T12044 punct -,galactopyranoside
R7960 T12048 T12044 nummod 3,galactopyranoside
R7961 T12049 T12044 punct -,galactopyranoside
R7962 T12050 T12044 compound indoxyl,galactopyranoside
R7963 T12051 T12044 punct -,galactopyranoside
R7964 T12052 T12044 compound beta,galactopyranoside
R7965 T12053 T12044 punct -,galactopyranoside
R7966 T12054 T12044 compound D,galactopyranoside
R7967 T12055 T12044 punct -,galactopyranoside
R7968 T12056 T12057 punct (,International
R7969 T12057 T12037 parataxis International,is
R7970 T12058 T12057 compound Biosynth,International
R7971 T12059 T12057 punct ", ",International
R7972 T12060 T12057 npadvmod Naperville,International
R7973 T12061 T12057 punct ", ",International
R7974 T12062 T12057 npadvmod Illinois,International
R7975 T12063 T12057 punct ", ",International
R7976 T12064 T12065 compound United,States
R7977 T12065 T12057 npadvmod States,International
R7978 T12066 T12057 punct ),International
R7979 T12067 T12037 punct .,is
R7980 T12177 T12178 amod General,characterization
R7981 T12179 T12178 prep of,characterization
R7982 T12180 T12181 npadvmod Bmpr1a,mutant
R7983 T12181 T12182 amod mutant,mice
R7984 T12182 T12179 pobj mice,of
R7985 T12184 T12185 npadvmod Bmpr1a,null
R7986 T12185 T12186 amod null,alleles
R7987 T12186 T12189 nsubjpass alleles,obtained
R7988 T12187 T12185 cc and,null
R7989 T12188 T12185 conj floxed,null
R7990 T12190 T12191 punct (,Ahn
R7991 T12191 T12186 meta Ahn,alleles
R7992 T12192 T12191 nmod et,Ahn
R7993 T12193 T12191 nmod al.,Ahn
R7994 T12194 T12191 nummod 2001,Ahn
R7995 T12195 T12191 punct ;,Ahn
R7996 T12196 T12191 nmod Mishina,Ahn
R7997 T12197 T12191 nmod et,Ahn
R7998 T12198 T12191 nmod al.,Ahn
R7999 T12199 T12191 nummod 2002,Ahn
R8000 T12200 T12191 punct ),Ahn
R8001 T12201 T12189 auxpass were,obtained
R8002 T12202 T12189 prep on,obtained
R8003 T12203 T12204 det a,background
R8004 T12204 T12202 pobj background,on
R8005 T12205 T12204 amod mixed,background
R8006 T12206 T12204 nummod 129,background
R8007 T12207 T12206 cc and,129
R8008 T12208 T12206 conj C57BL,129
R8009 T12209 T12208 punct /,C57BL
R8010 T12210 T12208 nummod 6,C57BL
R8011 T12211 T12189 cc and,obtained
R8012 T12212 T12189 conj maintained,obtained
R8013 T12213 T12212 prep by,maintained
R8014 T12214 T12215 amod random,breeding
R8015 T12215 T12213 pobj breeding,by
R8016 T12216 T12189 punct .,obtained
R8017 T12218 T12219 nsubjpass Mice,mated
R8018 T12220 T12218 acl carrying,Mice
R8019 T12221 T12222 det the,alleles
R8020 T12222 T12220 dobj alleles,carrying
R8021 T12223 T12222 amod null,alleles
R8022 T12224 T12223 cc and,null
R8023 T12225 T12223 conj floxed,null
R8024 T12226 T12219 auxpass were,mated
R8025 T12227 T12219 advmod typically,mated
R8026 T12228 T12219 prep to,mated
R8027 T12229 T12230 compound Gdf5,Cre
R8028 T12230 T12232 compound Cre,mice
R8029 T12231 T12230 punct -,Cre
R8030 T12232 T12228 pobj mice,to
R8031 T12233 T12234 mark as,shown
R8032 T12234 T12219 advcl shown,mated
R8033 T12235 T12234 prep in,shown
R8034 T12236 T12235 pobj Figure,in
R8035 T12237 T12236 nummod 3,Figure
R8036 T12238 T12219 punct .,mated
R8037 T12240 T12241 det The,mice
R8038 T12241 T12243 nsubj mice,are
R8039 T12242 T12241 amod resulting,mice
R8040 T12244 T12243 prep on,are
R8041 T12245 T12246 det a,background
R8042 T12246 T12244 pobj background,on
R8043 T12247 T12246 amod mixed,background
R8044 T12248 T12246 nummod 129,background
R8045 T12249 T12248 punct ;,129
R8046 T12250 T12248 appos C57Bl,129
R8047 T12251 T12250 punct /,C57Bl
R8048 T12252 T12250 nummod 6,C57Bl
R8049 T12253 T12248 punct ;,129
R8050 T12254 T12255 compound FVB,N
R8051 T12255 T12248 appos N,129
R8052 T12256 T12255 punct /,N
R8053 T12257 T12243 punct ", ",are
R8054 T12258 T12259 mark with,generated
R8055 T12259 T12243 advcl generated,are
R8056 T12260 T12261 preconj both,controls
R8057 T12261 T12259 nsubj controls,generated
R8058 T12262 T12261 cc and,controls
R8059 T12263 T12264 compound mutant,animals
R8060 T12264 T12261 conj animals,controls
R8061 T12265 T12259 prep as,generated
R8062 T12266 T12265 pobj littermates,as
R8063 T12267 T12266 prep from,littermates
R8064 T12268 T12269 det the,matings
R8065 T12269 T12267 pobj matings,from
R8066 T12270 T12269 amod same,matings
R8067 T12271 T12243 punct .,are
R8068 T12273 T12274 amod Whole,mount
R8069 T12274 T12276 nmod mount,preparations
R8070 T12275 T12274 punct -,mount
R8071 T12276 T12278 nsubjpass preparations,made
R8072 T12277 T12276 amod skeletal,preparations
R8073 T12279 T12278 auxpass were,made
R8074 T12280 T12278 prep from,made
R8075 T12281 T12282 quantmod 34,36
R8076 T12282 T12285 nummod 36,d
R8077 T12283 T12282 punct -,36
R8078 T12284 T12282 quantmod to,36
R8079 T12285 T12287 npadvmod d,old
R8080 T12286 T12285 punct -,d
R8081 T12287 T12289 amod old,mice
R8082 T12288 T12287 punct -,old
R8083 T12289 T12280 pobj mice,from
R8084 T12290 T12291 punct (,Lufkin
R8085 T12291 T12278 meta Lufkin,made
R8086 T12292 T12291 nmod et,Lufkin
R8087 T12293 T12291 nmod al.,Lufkin
R8088 T12294 T12291 nummod 1992,Lufkin
R8089 T12295 T12291 punct ),Lufkin
R8090 T12296 T12278 punct .,made
R8091 T12298 T12299 nsubjpass Pairs,removed
R8092 T12300 T12298 prep of,Pairs
R8093 T12301 T12300 pobj ears,of
R8094 T12302 T12298 prep from,Pairs
R8095 T12303 T12304 amod euthanized,animals
R8096 T12304 T12302 pobj animals,from
R8097 T12305 T12306 nummod 6,mo
R8098 T12306 T12308 npadvmod mo,old
R8099 T12307 T12306 punct -,mo
R8100 T12308 T12304 amod old,animals
R8101 T12309 T12308 punct -,old
R8102 T12310 T12299 auxpass were,removed
R8103 T12311 T12299 punct ", ",removed
R8104 T12312 T12299 conj pinned,removed
R8105 T12313 T12312 punct ", ",pinned
R8106 T12314 T12312 conj photographed,pinned
R8107 T12315 T12314 punct ", ",photographed
R8108 T12316 T12314 conj projected,photographed
R8109 T12317 T12316 punct ", ",projected
R8110 T12318 T12316 cc and,projected
R8111 T12319 T12316 conj measured,projected
R8112 T12320 T12319 prep from,measured
R8113 T12321 T12322 det the,base
R8114 T12322 T12320 pobj base,from
R8115 T12323 T12322 prep of,base
R8116 T12324 T12325 det the,curve
R8117 T12325 T12323 pobj curve,of
R8118 T12326 T12325 acl formed,curve
R8119 T12327 T12326 prep between,formed
R8120 T12328 T12329 det the,tragus
R8121 T12329 T12327 pobj tragus,between
R8122 T12330 T12329 cc and,tragus
R8123 T12331 T12329 conj antitragus,tragus
R8124 T12332 T12320 prep to,from
R8125 T12333 T12334 det the,point
R8126 T12334 T12332 pobj point,to
R8127 T12335 T12334 amod farthest,point
R8128 T12336 T12334 prep at,point
R8129 T12337 T12338 det the,edge
R8130 T12338 T12336 pobj edge,at
R8131 T12339 T12338 prep of,edge
R8132 T12340 T12341 det the,pinnae
R8133 T12341 T12339 pobj pinnae,of
R8134 T12342 T12299 punct .,removed
R8135 T12344 T12345 compound Grasping,ability
R8136 T12345 T12346 nsubjpass ability,measured
R8137 T12347 T12345 prep in,ability
R8138 T12348 T12349 nummod 6,mo
R8139 T12349 T12351 npadvmod mo,old
R8140 T12350 T12349 punct -,mo
R8141 T12351 T12353 amod old,mice
R8142 T12352 T12351 punct -,old
R8143 T12353 T12347 pobj mice,in
R8144 T12354 T12346 auxpass was,measured
R8145 T12355 T12346 prep by,measured
R8146 T12356 T12355 pcomp placing,by
R8147 T12357 T12356 dobj animals,placing
R8148 T12358 T12356 prep on,placing
R8149 T12359 T12360 det a,rod
R8150 T12360 T12358 pobj rod,on
R8151 T12361 T12360 amod slender,rod
R8152 T12362 T12356 cc and,placing
R8153 T12363 T12356 conj timing,placing
R8154 T12364 T12365 advmod how,long
R8155 T12365 T12366 advmod long,remain
R8156 T12366 T12363 ccomp remain,timing
R8157 T12367 T12366 nsubj they,remain
R8158 T12368 T12366 aux could,remain
R8159 T12369 T12366 oprd suspended,remain
R8160 T12370 T12369 prep on,suspended
R8161 T12371 T12372 det the,rod
R8162 T12372 T12370 pobj rod,on
R8163 T12373 T12363 punct ", ",timing
R8164 T12374 T12363 prep to,timing
R8165 T12375 T12376 det a,time
R8166 T12376 T12374 pobj time,to
R8167 T12377 T12376 amod maximum,time
R8168 T12378 T12376 acl allowed,time
R8169 T12379 T12376 prep of,time
R8170 T12380 T12381 nummod 2,min
R8171 T12381 T12379 pobj min,of
R8172 T12382 T12346 punct .,measured
R8173 T12384 T12385 nsubjpass Data,averaged
R8174 T12386 T12384 prep from,Data
R8175 T12387 T12388 nummod five,trials
R8176 T12388 T12386 pobj trials,from
R8177 T12389 T12388 amod consecutive,trials
R8178 T12390 T12388 prep for,trials
R8179 T12391 T12392 det each,mouse
R8180 T12392 T12390 pobj mouse,for
R8181 T12393 T12385 auxpass were,averaged
R8182 T12394 T12385 punct .,averaged
R8183 T12396 T12397 nmod Range,assays
R8184 T12397 T12400 nsubjpass assays,conducted
R8185 T12398 T12396 prep of,Range
R8186 T12399 T12398 pobj motion,of
R8187 T12401 T12400 auxpass were,conducted
R8188 T12402 T12400 prep on,conducted
R8189 T12403 T12404 det the,joints
R8190 T12404 T12402 pobj joints,on
R8191 T12405 T12406 nmod MT,P1
R8192 T12406 T12404 nmod P1,joints
R8193 T12407 T12406 punct /,P1
R8194 T12408 T12406 cc and,P1
R8195 T12409 T12410 compound P1,P2
R8196 T12410 T12406 conj P2,P1
R8197 T12411 T12410 punct /,P2
R8198 T12412 T12404 prep of,joints
R8199 T12413 T12414 det the,digit
R8200 T12414 T12412 pobj digit,of
R8201 T12415 T12414 amod second,digit
R8202 T12416 T12414 compound hindlimb,digit
R8203 T12417 T12404 prep from,joints
R8204 T12418 T12419 amod euthanized,animals
R8205 T12419 T12417 pobj animals,from
R8206 T12420 T12421 nummod 18,wk
R8207 T12421 T12423 npadvmod wk,old
R8208 T12422 T12421 punct -,wk
R8209 T12423 T12419 amod old,animals
R8210 T12424 T12423 punct -,old
R8211 T12425 T12400 punct .,conducted
R8212 T12427 T12428 nsubjpass Forceps,used
R8213 T12429 T12428 auxpass were,used
R8214 T12430 T12431 aux to,bend
R8215 T12431 T12428 advcl bend,used
R8216 T12432 T12433 det the,joint
R8217 T12433 T12431 dobj joint,bend
R8218 T12434 T12431 prep to,bend
R8219 T12435 T12436 poss its,position
R8220 T12436 T12434 pobj position,to
R8221 T12437 T12436 amod natural,position
R8222 T12438 T12436 compound stopping,position
R8223 T12439 T12428 punct ", ",used
R8224 T12440 T12428 cc and,used
R8225 T12441 T12442 det the,angle
R8226 T12442 T12444 nsubjpass angle,measured
R8227 T12443 T12442 amod resulting,angle
R8228 T12444 T12428 conj measured,used
R8229 T12445 T12444 auxpass was,measured
R8230 T12446 T12444 prep to,measured
R8231 T12447 T12448 det the,°
R8232 T12448 T12446 pobj °,to
R8233 T12449 T12448 amod nearest,°
R8234 T12450 T12448 nummod 10,°
R8235 T12451 T12444 prep under,measured
R8236 T12452 T12453 nummod 12.5,magnification
R8237 T12453 T12451 pobj magnification,under
R8238 T12454 T12452 punct ×,12.5
R8239 T12455 T12444 advcl using,measured
R8240 T12456 T12457 det a,reticule
R8241 T12457 T12455 dobj reticule,using
R8242 T12458 T12459 nummod 360,°
R8243 T12459 T12457 compound °,reticule
R8244 T12460 T12444 punct .,measured
R8245 T12462 T12463 nsubj Analysis,occurred
R8246 T12464 T12462 acl described,Analysis
R8247 T12465 T12464 prep in,described
R8248 T12466 T12467 det this,section
R8249 T12467 T12465 pobj section,in
R8250 T12468 T12463 prep on,occurred
R8251 T12469 T12468 pobj animals,on
R8252 T12470 T12469 acl lacking,animals
R8253 T12471 T12470 dobj R26R,lacking
R8254 T12472 T12463 punct .,occurred
R8255 T12474 T12475 compound Control,mice
R8256 T12475 T12476 nsubj mice,included
R8257 T12477 T12478 det all,genotypes
R8258 T12478 T12476 dobj genotypes,included
R8259 T12479 T12478 amod nonmutant,genotypes
R8260 T12480 T12478 acl generated,genotypes
R8261 T12481 T12480 prep by,generated
R8262 T12482 T12483 nsubj Parent,being
R8263 T12483 T12481 pobj being,by
R8264 T12484 T12482 nummod 1,Parent
R8265 T12485 T12483 acomp heterozygous,being
R8266 T12486 T12485 prep for,heterozygous
R8267 T12487 T12488 compound Gdf5,Cre
R8268 T12488 T12486 pobj Cre,for
R8269 T12489 T12488 punct -,Cre
R8270 T12490 T12488 cc and,Cre
R8271 T12491 T12488 conj Bmpr1anull,Cre
R8272 T12492 T12483 cc and,being
R8273 T12493 T12494 nsubj Parent,being
R8274 T12494 T12483 conj being,being
R8275 T12495 T12493 nummod 2,Parent
R8276 T12496 T12494 acomp heterozygous,being
R8277 T12497 T12496 prep for,heterozygous
R8278 T12498 T12497 pobj Bmpr1afloxP,for
R8279 T12499 T12500 punct (,see
R8280 T12500 T12494 parataxis see,being
R8281 T12501 T12500 dobj Figure,see
R8282 T12502 T12501 nummod 3,Figure
R8283 T12503 T12500 punct ),see
R8284 T12504 T12476 punct .,included
R8285 T12506 T12507 det All,analysis
R8286 T12507 T12509 nsubj analysis,used
R8287 T12508 T12507 amod statistical,analysis
R8288 T12510 T12511 det the,Student
R8289 T12511 T12512 poss Student,test
R8290 T12512 T12509 dobj test,used
R8291 T12513 T12511 case 's,Student
R8292 T12514 T12512 compound t,test
R8293 T12515 T12512 punct -,test
R8294 T12516 T12512 cc or,test
R8295 T12517 T12518 poss Welch,test
R8296 T12518 T12512 conj test,test
R8297 T12519 T12517 case 's,Welch
R8298 T12520 T12518 compound t,test
R8299 T12521 T12518 punct -,test
R8300 T12522 T12509 punct ", ",used
R8301 T12523 T12509 cc and,used
R8302 T12524 T12525 nsubj values,are
R8303 T12525 T12509 conj are,used
R8304 T12526 T12524 acl listed,values
R8305 T12527 T12525 attr mean,are
R8306 T12528 T12527 punct ±,mean
R8307 T12529 T12530 amod standard,error
R8308 T12530 T12527 appos error,mean
R8309 T12531 T12530 prep of,error
R8310 T12532 T12533 det the,mean
R8311 T12533 T12531 pobj mean,of
R8312 T12534 T12525 punct .,are
R8319 T12651 T12652 compound Cell,death
R8320 T12653 T12652 cc and,death
R8321 T12654 T12655 compound proliferation,assays
R8322 T12655 T12652 conj assays,death
R8323 T12657 T12658 nsubjpass Limbs,dissected
R8324 T12659 T12657 prep from,Limbs
R8325 T12660 T12661 amod mutant,animals
R8326 T12661 T12659 pobj animals,from
R8327 T12662 T12660 cc and,mutant
R8328 T12663 T12660 conj control,mutant
R8329 T12664 T12657 prep at,Limbs
R8330 T12665 T12664 pobj E13.5,at
R8331 T12666 T12665 cc and,E13.5
R8332 T12667 T12665 conj E14.5,E13.5
R8333 T12668 T12658 auxpass were,dissected
R8334 T12669 T12658 cc and,dissected
R8335 T12670 T12658 conj frozen,dissected
R8336 T12671 T12670 prep in,frozen
R8337 T12672 T12671 pobj OCT,in
R8338 T12673 T12674 punct (,Finetek
R8339 T12674 T12670 parataxis Finetek,frozen
R8340 T12675 T12674 compound Sakura,Finetek
R8341 T12676 T12674 punct ",",Finetek
R8342 T12677 T12674 npadvmod Torrence,Finetek
R8343 T12678 T12674 punct ", ",Finetek
R8344 T12679 T12674 npadvmod CA,Finetek
R8345 T12680 T12674 punct ", ",Finetek
R8346 T12681 T12682 compound United,States
R8347 T12682 T12674 npadvmod States,Finetek
R8348 T12683 T12674 punct ),Finetek
R8349 T12684 T12658 punct .,dissected
R8350 T12686 T12687 nsubjpass Cryosections,assayed
R8351 T12688 T12686 prep of,Cryosections
R8352 T12689 T12688 pobj tissue,of
R8353 T12690 T12687 auxpass were,assayed
R8354 T12691 T12687 prep by,assayed
R8355 T12692 T12691 pobj TUNEL,by
R8356 T12693 T12687 advcl using,assayed
R8357 T12694 T12695 det the,Kit
R8358 T12695 T12693 dobj Kit,using
R8359 T12696 T12697 advmod In,Situ
R8360 T12697 T12698 amod Situ,Detection
R8361 T12698 T12695 compound Detection,Kit
R8362 T12699 T12700 compound Cell,Death
R8363 T12700 T12698 compound Death,Detection
R8364 T12701 T12695 punct ", ",Kit
R8365 T12702 T12695 npadvmod Fluorescein,Kit
R8366 T12703 T12702 punct (,Fluorescein
R8367 T12704 T12702 npadvmod Roche,Fluorescein
R8368 T12705 T12702 punct ", ",Fluorescein
R8369 T12706 T12702 npadvmod Basel,Fluorescein
R8370 T12707 T12702 punct ", ",Fluorescein
R8371 T12708 T12702 npadvmod Switzerland,Fluorescein
R8372 T12709 T12702 punct ),Fluorescein
R8373 T12710 T12687 punct .,assayed
R8374 T12712 T12713 prep Following,washed
R8375 T12714 T12712 pobj TUNEL,Following
R8376 T12715 T12713 punct ", ",washed
R8377 T12716 T12713 nsubjpass slides,washed
R8378 T12717 T12713 auxpass were,washed
R8379 T12718 T12713 prep in,washed
R8380 T12719 T12718 pobj PBS,in
R8381 T12720 T12713 punct ", ",washed
R8382 T12721 T12713 conj blocked,washed
R8383 T12722 T12721 prep with,blocked
R8384 T12723 T12722 pobj PBS,with
R8385 T12724 T12723 punct +,PBS
R8386 T12725 T12726 nummod 0.05,%
R8387 T12726 T12727 compound %,Tween
R8388 T12727 T12723 appos Tween,PBS
R8389 T12728 T12727 punct -,Tween
R8390 T12729 T12727 nummod 20,Tween
R8391 T12730 T12723 punct +,PBS
R8392 T12731 T12732 nummod 5,%
R8393 T12732 T12733 compound %,serum
R8394 T12733 T12723 appos serum,PBS
R8395 T12734 T12733 compound goat,serum
R8396 T12735 T12721 punct ", ",blocked
R8397 T12736 T12721 conj washed,blocked
R8398 T12737 T12736 advmod again,washed
R8399 T12738 T12736 punct ", ",washed
R8400 T12739 T12736 cc and,washed
R8401 T12740 T12736 conj incubated,washed
R8402 T12741 T12740 prep with,incubated
R8403 T12742 T12743 det a,dilution
R8404 T12743 T12741 pobj dilution,with
R8405 T12744 T12743 nummod 1,dilution
R8406 T12745 T12746 punct :,200
R8407 T12746 T12744 prep 200,1
R8408 T12747 T12743 prep of,dilution
R8409 T12748 T12749 det a,antibody
R8410 T12749 T12747 pobj antibody,of
R8411 T12750 T12749 nmod rabbit,antibody
R8412 T12751 T12752 amod anti-phospho-histone,H3
R8413 T12752 T12749 compound H3,antibody
R8414 T12753 T12752 punct -,H3
R8415 T12754 T12749 acl called,antibody
R8416 T12755 T12756 compound Mitosis,Marker
R8417 T12756 T12754 oprd Marker,called
R8418 T12757 T12758 punct (,Biotechnology
R8419 T12758 T12756 parataxis Biotechnology,Marker
R8420 T12759 T12758 compound Upstate,Biotechnology
R8421 T12760 T12758 punct ", ",Biotechnology
R8422 T12761 T12762 compound Lake,Placid
R8423 T12762 T12758 npadvmod Placid,Biotechnology
R8424 T12763 T12758 punct ", ",Biotechnology
R8425 T12764 T12765 compound New,York
R8426 T12765 T12758 npadvmod York,Biotechnology
R8427 T12766 T12758 punct ", ",Biotechnology
R8428 T12767 T12768 compound United,States
R8429 T12768 T12758 npadvmod States,Biotechnology
R8430 T12769 T12758 punct ),Biotechnology
R8431 T12770 T12771 aux to,identify
R8432 T12771 T12740 advcl identify,incubated
R8433 T12772 T12771 dobj cells,identify
R8434 T12773 T12772 prep in,cells
R8435 T12774 T12773 pobj mitosis,in
R8436 T12775 T12713 punct .,washed
R8437 T12777 T12778 npadvmod Cy3,labeled
R8438 T12778 T12780 amod labeled,antibody
R8439 T12779 T12778 punct -,labeled
R8440 T12780 T12783 nsubjpass antibody,used
R8441 T12781 T12780 amod anti-rabbit,antibody
R8442 T12782 T12780 amod secondary,antibody
R8443 T12784 T12783 auxpass was,used
R8444 T12785 T12786 aux to,detect
R8445 T12786 T12783 advcl detect,used
R8446 T12787 T12788 det the,antibody
R8447 T12788 T12786 dobj antibody,detect
R8448 T12789 T12783 punct .,used
R8449 T12791 T12792 compound Cell,nuclei
R8450 T12792 T12793 nsubjpass nuclei,labeled
R8451 T12794 T12793 auxpass were,labeled
R8452 T12795 T12793 prep with,labeled
R8453 T12796 T12795 pobj DAPI,with
R8454 T12797 T12793 punct ", ",labeled
R8455 T12798 T12793 cc and,labeled
R8456 T12799 T12800 nsubjpass slides,mounted
R8457 T12800 T12793 conj mounted,labeled
R8458 T12801 T12800 auxpass were,mounted
R8459 T12802 T12800 prep in,mounted
R8460 T12803 T12802 pobj Vectamount,in
R8461 T12804 T12805 punct (,Laboratories
R8462 T12805 T12803 parataxis Laboratories,Vectamount
R8463 T12806 T12805 compound Vector,Laboratories
R8464 T12807 T12805 punct ", ",Laboratories
R8465 T12808 T12805 npadvmod Burlingame,Laboratories
R8466 T12809 T12805 punct ", ",Laboratories
R8467 T12810 T12805 npadvmod California,Laboratories
R8468 T12811 T12805 punct ", ",Laboratories
R8469 T12812 T12813 compound United,States
R8470 T12813 T12805 npadvmod States,Laboratories
R8471 T12814 T12805 punct ),Laboratories
R8472 T12815 T12800 cc and,mounted
R8473 T12816 T12800 conj visualized,mounted
R8474 T12817 T12816 prep at,visualized
R8475 T12818 T12819 nummod 100,magnification
R8476 T12819 T12817 pobj magnification,at
R8477 T12820 T12818 punct ×,100
R8478 T12821 T12800 punct .,mounted
R8479 T12823 T12824 det The,area
R8480 T12824 T12825 nsubjpass area,measured
R8481 T12826 T12824 prep of,area
R8482 T12827 T12828 amod selected,sites
R8483 T12828 T12826 pobj sites,of
R8484 T12829 T12828 amod anatomical,sites
R8485 T12830 T12825 auxpass were,measured
R8486 T12831 T12825 punct ", ",measured
R8487 T12832 T12825 cc and,measured
R8488 T12833 T12834 det the,number
R8489 T12834 T12835 nsubjpass number,counted
R8490 T12835 T12825 conj counted,measured
R8491 T12836 T12834 prep of,number
R8492 T12837 T12838 npadvmod TUNEL,labeled
R8493 T12838 T12840 amod labeled,fragments
R8494 T12839 T12838 punct -,labeled
R8495 T12840 T12836 pobj fragments,of
R8496 T12841 T12840 amod nuclear,fragments
R8497 T12842 T12834 cc and,number
R8498 T12843 T12844 det the,number
R8499 T12844 T12834 conj number,number
R8500 T12845 T12844 prep of,number
R8501 T12846 T12847 npadvmod Cy3,labeled
R8502 T12847 T12849 amod labeled,nuclei
R8503 T12848 T12847 punct -,labeled
R8504 T12849 T12845 pobj nuclei,of
R8505 T12850 T12835 auxpass were,counted
R8506 T12851 T12835 prep from,counted
R8507 T12852 T12853 nummod three,sections
R8508 T12853 T12851 pobj sections,from
R8509 T12854 T12855 nummod 10,μm
R8510 T12855 T12853 compound μm,sections
R8511 T12856 T12855 punct -,μm
R8512 T12857 T12853 acl spanning,sections
R8513 T12858 T12859 nummod 50,μm
R8514 T12859 T12857 dobj μm,spanning
R8515 T12860 T12853 punct ", ",sections
R8516 T12861 T12853 prep from,sections
R8517 T12862 T12863 nummod three,control
R8518 T12863 T12861 pobj control,from
R8519 T12864 T12863 cc and,control
R8520 T12865 T12866 nummod three,mutant
R8521 T12866 T12867 compound mutant,animals
R8522 T12867 T12863 conj animals,control
R8523 T12868 T12835 punct .,counted
R8524 T12870 T12871 det The,number
R8525 T12871 T12872 nsubjpass number,counted
R8526 T12873 T12871 prep of,number
R8527 T12874 T12875 amod labeled,cells
R8528 T12875 T12873 pobj cells,of
R8529 T12876 T12871 prep in,number
R8530 T12877 T12878 det the,joints
R8531 T12878 T12876 pobj joints,in
R8532 T12879 T12880 amod metacarpal,phalangeal
R8533 T12880 T12878 amod phalangeal,joints
R8534 T12881 T12880 punct -,phalangeal
R8535 T12882 T12880 cc and,phalangeal
R8536 T12883 T12884 amod metatarsal,phalangeal
R8537 T12884 T12880 conj phalangeal,phalangeal
R8538 T12885 T12884 punct -,phalangeal
R8539 T12886 T12872 auxpass was,counted
R8540 T12887 T12872 prep in,counted
R8541 T12888 T12889 det a,rectangle
R8542 T12889 T12887 pobj rectangle,in
R8543 T12890 T12891 nummod 290,μm
R8544 T12891 T12889 nmod μm,rectangle
R8545 T12892 T12893 punct ×,μm
R8546 T12893 T12891 prep μm,μm
R8547 T12894 T12893 nummod 365,μm
R8548 T12895 T12889 acl placed,rectangle
R8549 T12896 T12895 prep around,placed
R8550 T12897 T12898 det the,center
R8551 T12898 T12896 pobj center,around
R8552 T12899 T12898 prep of,center
R8553 T12900 T12901 det the,joint
R8554 T12901 T12899 pobj joint,of
R8555 T12902 T12872 punct .,counted
R8556 T12904 T12905 det The,region
R8557 T12905 T12907 nsubjpass region,defined
R8558 T12906 T12905 amod posterior,region
R8559 T12908 T12905 prep of,region
R8560 T12909 T12910 det the,digit
R8561 T12910 T12908 pobj digit,of
R8562 T12911 T12910 amod fifth,digit
R8563 T12912 T12907 auxpass was,defined
R8564 T12913 T12907 prep by,defined
R8565 T12914 T12913 pcomp drawing,by
R8566 T12915 T12916 det a,line
R8567 T12916 T12914 dobj line,drawing
R8568 T12917 T12914 prep from,drawing
R8569 T12918 T12919 det the,tip
R8570 T12919 T12917 pobj tip,from
R8571 T12920 T12919 prep of,tip
R8572 T12921 T12922 det the,digit
R8573 T12922 T12920 pobj digit,of
R8574 T12923 T12914 prep down,drawing
R8575 T12924 T12925 nummod 2.15,mm
R8576 T12925 T12923 pobj mm,down
R8577 T12926 T12923 cc and,down
R8578 T12927 T12923 conj across,down
R8579 T12928 T12927 prep to,across
R8580 T12929 T12930 det the,edge
R8581 T12930 T12928 pobj edge,to
R8582 T12931 T12930 amod lateral,edge
R8583 T12932 T12930 prep of,edge
R8584 T12933 T12934 det the,tissue
R8585 T12934 T12932 pobj tissue,of
R8586 T12935 T12907 punct .,defined
R8587 T12937 T12938 prep For,was
R8588 T12939 T12940 det this,analysis
R8589 T12940 T12937 pobj analysis,For
R8590 T12941 T12938 punct ", ",was
R8591 T12942 T12943 det the,reporter
R8592 T12943 T12938 nsubj reporter,was
R8593 T12944 T12945 compound R26R,Cre
R8594 T12945 T12943 compound Cre,reporter
R8595 T12946 T12938 neg not,was
R8596 T12947 T12938 acomp present,was
R8597 T12948 T12938 punct .,was
R8598 T13138 T13137 cc and,Histology
R8599 T13139 T13137 conj histochemistry,Histology
R8600 T13141 T13142 nsubjpass Tissue,prepared
R8601 T13143 T13141 prep from,Tissue
R8602 T13144 T13143 pobj animals,from
R8603 T13145 T13144 acl ranging,animals
R8604 T13146 T13145 prep from,ranging
R8605 T13147 T13148 compound stages,E14.5
R8606 T13148 T13146 pobj E14.5,from
R8607 T13149 T13146 prep to,from
R8608 T13150 T13149 pobj P14,to
R8609 T13151 T13142 auxpass was,prepared
R8610 T13152 T13142 prep for,prepared
R8611 T13153 T13152 pobj analysis,for
R8612 T13154 T13142 prep by,prepared
R8613 T13155 T13154 pcomp fixing,by
R8614 T13156 T13155 prep in,fixing
R8615 T13157 T13158 nummod 4,%
R8616 T13158 T13159 compound %,paraformaldehyde
R8617 T13159 T13156 pobj paraformaldehyde,in
R8618 T13160 T13161 punct (,PFA
R8619 T13161 T13159 parataxis PFA,paraformaldehyde
R8620 T13162 T13161 punct ),PFA
R8621 T13163 T13159 prep in,paraformaldehyde
R8622 T13164 T13163 pobj PBS,in
R8623 T13165 T13155 prep for,fixing
R8624 T13166 T13167 nummod 45,min
R8625 T13167 T13165 pobj min,for
R8626 T13168 T13167 prep to,min
R8627 T13169 T13170 nummod 4,h
R8628 T13170 T13168 pobj h,to
R8629 T13171 T13167 prep depending,min
R8630 T13172 T13171 prep on,depending
R8631 T13173 T13174 det the,stage
R8632 T13174 T13172 pobj stage,on
R8633 T13175 T13155 punct ;,fixing
R8634 T13176 T13155 conj washing,fixing
R8635 T13177 T13178 nummod three,times
R8636 T13178 T13176 npadvmod times,washing
R8637 T13179 T13176 prep in,washing
R8638 T13180 T13179 pobj PBS,in
R8639 T13181 T13176 punct ", ",washing
R8640 T13182 T13176 conj once,washing
R8641 T13183 T13182 prep in,once
R8642 T13184 T13183 pobj PBS,in
R8643 T13185 T13184 punct +,PBS
R8644 T13186 T13187 nummod 15,%
R8645 T13187 T13188 compound %,sucrose
R8646 T13188 T13184 appos sucrose,PBS
R8647 T13189 T13182 prep for,once
R8648 T13190 T13191 nummod 1,h
R8649 T13191 T13189 pobj h,for
R8650 T13192 T13182 punct ", ",once
R8651 T13193 T13182 cc and,once
R8652 T13194 T13182 conj once,once
R8653 T13195 T13194 prep in,once
R8654 T13196 T13195 pobj PBS,in
R8655 T13197 T13196 punct +,PBS
R8656 T13198 T13199 nummod 30,%
R8657 T13199 T13200 compound %,sucrose
R8658 T13200 T13196 appos sucrose,PBS
R8659 T13201 T13194 prep for,once
R8660 T13202 T13203 nummod 2,h
R8661 T13203 T13201 pobj h,for
R8662 T13204 T13203 prep to,h
R8663 T13205 T13204 pobj overnight,to
R8664 T13206 T13203 prep depending,h
R8665 T13207 T13206 prep on,depending
R8666 T13208 T13209 det the,stage
R8667 T13209 T13207 pobj stage,on
R8668 T13210 T13176 punct ;,washing
R8669 T13211 T13176 cc and,washing
R8670 T13212 T13213 advmod then,freezing
R8671 T13213 T13176 conj freezing,washing
R8672 T13214 T13213 prep in,freezing
R8673 T13215 T13214 pobj OCT,in
R8674 T13216 T13142 punct .,prepared
R8675 T13218 T13219 nsubjpass Tissue,processed
R8676 T13220 T13218 prep from,Tissue
R8677 T13221 T13220 pobj animals,from
R8678 T13222 T13221 acl aged,animals
R8679 T13223 T13224 nummod 7,wk
R8680 T13224 T13222 npadvmod wk,aged
R8681 T13225 T13224 prep to,wk
R8682 T13226 T13227 nummod 9,mo
R8683 T13227 T13225 pobj mo,to
R8684 T13228 T13219 auxpass was,processed
R8685 T13229 T13219 advmod similarly,processed
R8686 T13230 T13229 prep to,similarly
R8687 T13231 T13232 amod earlier,stages
R8688 T13232 T13230 pobj stages,to
R8689 T13233 T13219 prep except,processed
R8690 T13234 T13235 mark that,decalcified
R8691 T13235 T13233 pcomp decalcified,except
R8692 T13236 T13235 nsubjpass it,decalcified
R8693 T13237 T13235 auxpass was,decalcified
R8694 T13238 T13235 prep in,decalcified
R8695 T13239 T13240 nummod 0.5,M
R8696 T13240 T13241 compound M,EDTA
R8697 T13241 T13238 pobj EDTA,in
R8698 T13242 T13241 punct (,EDTA
R8699 T13243 T13241 npadvmod pH,EDTA
R8700 T13244 T13243 nummod 7.4,pH
R8701 T13245 T13241 punct ),EDTA
R8702 T13246 T13235 prep for,decalcified
R8703 T13247 T13248 nummod 4,d
R8704 T13248 T13246 pobj d,for
R8705 T13249 T13250 amod prior,to
R8706 T13250 T13235 prep to,decalcified
R8707 T13251 T13250 pcomp incubating,to
R8708 T13252 T13251 prep in,incubating
R8709 T13253 T13252 pobj sucrose,in
R8710 T13254 T13219 punct .,processed
R8711 T13256 T13257 det All,solutions
R8712 T13257 T13258 nsubjpass solutions,prechilled
R8713 T13259 T13258 auxpass were,prechilled
R8714 T13260 T13258 cc and,prechilled
R8715 T13261 T13258 conj used,prechilled
R8716 T13262 T13261 prep at,used
R8717 T13263 T13264 nummod 4,°C
R8718 T13264 T13262 pobj °C,at
R8719 T13265 T13261 prep with,used
R8720 T13266 T13265 pobj agitation,with
R8721 T13267 T13258 punct ", ",prechilled
R8722 T13268 T13258 cc and,prechilled
R8723 T13269 T13270 nsubjpass skin,lacerated
R8724 T13270 T13258 conj lacerated,prechilled
R8725 T13271 T13269 prep from,skin
R8726 T13272 T13271 pobj tissues,from
R8727 T13273 T13272 prep of,tissues
R8728 T13274 T13275 nmod P0,mice
R8729 T13275 T13273 pobj mice,of
R8730 T13276 T13274 cc or,P0
R8731 T13277 T13274 conj older,P0
R8732 T13278 T13270 auxpass was,lacerated
R8733 T13279 T13270 cc or,lacerated
R8734 T13280 T13270 conj removed,lacerated
R8735 T13281 T13282 amod prior,to
R8736 T13282 T13280 prep to,removed
R8737 T13283 T13282 pobj processing,to
R8738 T13284 T13270 punct .,lacerated
R8739 T13286 T13287 nsubjpass Tissue,cryosectioned
R8740 T13288 T13287 auxpass was,cryosectioned
R8741 T13289 T13287 advmod then,cryosectioned
R8742 T13290 T13287 prep at,cryosectioned
R8743 T13291 T13292 nummod 12,μm
R8744 T13292 T13290 pobj μm,at
R8745 T13293 T13287 cc and,cryosectioned
R8746 T13294 T13287 conj processed,cryosectioned
R8747 T13295 T13287 punct .,cryosectioned
R8748 T13297 T13298 nsubjpass Staining,carried
R8749 T13299 T13297 prep of,Staining
R8750 T13300 T13299 pobj sections,of
R8751 T13301 T13297 prep with,Staining
R8752 T13302 T13303 compound Safranin,O
R8753 T13303 T13301 pobj O,with
R8754 T13304 T13303 punct ", ",O
R8755 T13305 T13306 amod Fast,Green
R8756 T13306 T13303 conj Green,O
R8757 T13307 T13306 punct ", ",Green
R8758 T13308 T13306 cc and,Green
R8759 T13309 T13310 poss Harris,hematoxylin
R8760 T13310 T13306 conj hematoxylin,Green
R8761 T13311 T13309 case ',Harris
R8762 T13312 T13298 auxpass was,carried
R8763 T13313 T13298 prt out,carried
R8764 T13314 T13298 advcl using,carried
R8765 T13315 T13316 amod standard,procedures
R8766 T13316 T13314 dobj procedures,using
R8767 T13317 T13316 amod histological,procedures
R8768 T13318 T13298 punct .,carried
R8769 T13320 T13321 nsubjpass Detection,performed
R8770 T13322 T13320 prep of,Detection
R8771 T13323 T13324 compound LACZ,activity
R8772 T13324 T13322 pobj activity,of
R8773 T13325 T13320 prep with,Detection
R8774 T13326 T13327 compound X,Gal
R8775 T13327 T13325 pobj Gal,with
R8776 T13328 T13327 punct -,Gal
R8777 T13329 T13321 auxpass was,performed
R8778 T13330 T13331 mark as,described
R8779 T13331 T13321 advcl described,performed
R8780 T13332 T13333 punct (,Lobe
R8781 T13333 T13331 meta Lobe,described
R8782 T13334 T13333 nmod et,Lobe
R8783 T13335 T13333 nmod al.,Lobe
R8784 T13336 T13333 nummod 1999,Lobe
R8785 T13337 T13333 punct ),Lobe
R8786 T13338 T13321 cc and,performed
R8787 T13339 T13340 auxpass was,followed
R8788 T13340 T13321 conj followed,performed
R8789 T13341 T13340 agent by,followed
R8790 T13342 T13341 pcomp refixing,by
R8791 T13343 T13342 prep in,refixing
R8792 T13344 T13345 nummod 4,%
R8793 T13345 T13346 compound %,PFA
R8794 T13346 T13343 pobj PFA,in
R8795 T13347 T13342 punct ", ",refixing
R8796 T13348 T13342 conj rinsing,refixing
R8797 T13349 T13348 prep with,rinsing
R8798 T13350 T13351 amod deionized,water
R8799 T13351 T13349 pobj water,with
R8800 T13352 T13348 punct ", ",rinsing
R8801 T13353 T13348 conj counterstaining,rinsing
R8802 T13354 T13353 prep with,counterstaining
R8803 T13355 T13356 amod Nuclear,Red
R8804 T13356 T13354 pobj Red,with
R8805 T13357 T13356 amod Fast,Red
R8806 T13358 T13356 punct (,Red
R8807 T13359 T13360 compound Vector,Labs
R8808 T13360 T13356 npadvmod Labs,Red
R8809 T13361 T13356 punct ),Red
R8810 T13362 T13353 punct ", ",counterstaining
R8811 T13363 T13353 conj rinsing,counterstaining
R8812 T13364 T13363 prep with,rinsing
R8813 T13365 T13364 pobj water,with
R8814 T13366 T13363 advmod again,rinsing
R8815 T13367 T13363 punct ", ",rinsing
R8816 T13368 T13363 cc and,rinsing
R8817 T13369 T13370 advmod then,mounting
R8818 T13370 T13363 conj mounting,rinsing
R8819 T13371 T13370 prep in,mounting
R8820 T13372 T13371 pobj Aquamount,in
R8821 T13373 T13374 punct (,Labs
R8822 T13374 T13372 parataxis Labs,Aquamount
R8823 T13375 T13374 compound Lerner,Labs
R8824 T13376 T13374 punct ", ",Labs
R8825 T13377 T13374 npadvmod Pittsburgh,Labs
R8826 T13378 T13374 punct ", ",Labs
R8827 T13379 T13374 npadvmod Pennsylvania,Labs
R8828 T13380 T13374 punct ", ",Labs
R8829 T13381 T13382 compound United,States
R8830 T13382 T13374 npadvmod States,Labs
R8831 T13383 T13374 punct ),Labs
R8832 T13384 T13321 punct .,performed
R8833 T13386 T13387 nmod RNA,hybridization
R8834 T13387 T13390 nsubjpass hybridization,performed
R8835 T13388 T13389 advmod in,situ
R8836 T13389 T13387 amod situ,hybridization
R8837 T13391 T13390 auxpass was,performed
R8838 T13392 T13393 mark as,described
R8839 T13393 T13390 advcl described,performed
R8840 T13394 T13395 punct (,Storm
R8841 T13395 T13393 meta Storm,described
R8842 T13396 T13395 cc and,Storm
R8843 T13397 T13395 conj Kingsley,Storm
R8844 T13398 T13397 nummod 1996,Kingsley
R8845 T13399 T13397 punct ),Kingsley
R8846 T13400 T13390 punct ", ",performed
R8847 T13401 T13390 prep with,performed
R8848 T13402 T13403 det the,modifications
R8849 T13403 T13401 pobj modifications,with
R8850 T13404 T13403 amod following,modifications
R8851 T13405 T13390 punct : ,performed
R8852 T13406 T13407 punct (,1
R8853 T13407 T13408 meta 1,incubated
R8854 T13408 T13390 conj incubated,performed
R8855 T13409 T13407 punct ),1
R8856 T13410 T13411 amod Prior,to
R8857 T13411 T13408 prep to,incubated
R8858 T13412 T13413 det the,step
R8859 T13413 T13411 pobj step,to
R8860 T13414 T13413 compound acetylation,step
R8861 T13415 T13408 punct ", ",incubated
R8862 T13416 T13408 nsubjpass sections,incubated
R8863 T13417 T13408 auxpass were,incubated
R8864 T13418 T13408 prep with,incubated
R8865 T13419 T13420 compound 10,20
R8866 T13420 T13422 nummod 20,μg
R8867 T13421 T13420 punct –,20
R8868 T13422 T13423 nmod μg,K
R8869 T13423 T13418 pobj K,with
R8870 T13424 T13425 punct /,ml
R8871 T13425 T13422 prep ml,μg
R8872 T13426 T13423 compound proteinase,K
R8873 T13427 T13408 prep for,incubated
R8874 T13428 T13429 nummod 30,s
R8875 T13429 T13427 pobj s,for
R8876 T13430 T13429 prep to,s
R8877 T13431 T13432 nummod 7,min
R8878 T13432 T13430 pobj min,to
R8879 T13433 T13408 prep at,incubated
R8880 T13434 T13435 compound room,temperature
R8881 T13435 T13433 pobj temperature,at
R8882 T13436 T13435 punct (,temperature
R8883 T13437 T13435 prep depending,temperature
R8884 T13438 T13437 prep on,depending
R8885 T13439 T13440 det the,stage
R8886 T13440 T13438 pobj stage,on
R8887 T13441 T13440 amod developmental,stage
R8888 T13442 T13435 punct ),temperature
R8889 T13443 T13408 punct ", ",incubated
R8890 T13444 T13408 advcl followed,incubated
R8891 T13445 T13444 agent by,followed
R8892 T13446 T13445 pcomp refixing,by
R8893 T13447 T13446 prep in,refixing
R8894 T13448 T13449 nummod 4,%
R8895 T13449 T13450 compound %,PFA
R8896 T13450 T13447 pobj PFA,in
R8897 T13451 T13446 cc and,refixing
R8898 T13452 T13446 conj washing,refixing
R8899 T13453 T13454 nummod three,times
R8900 T13454 T13452 npadvmod times,washing
R8901 T13455 T13452 prep in,washing
R8902 T13456 T13455 pobj PBS,in
R8903 T13457 T13408 punct ;,incubated
R8904 T13458 T13459 punct (,2
R8905 T13459 T13460 meta 2,skipped
R8906 T13460 T13408 conj skipped,incubated
R8907 T13461 T13459 punct ),2
R8908 T13462 T13463 compound prehybridization,step
R8909 T13463 T13460 nsubjpass step,skipped
R8910 T13464 T13460 auxpass was,skipped
R8911 T13465 T13460 punct ", ",skipped
R8912 T13466 T13460 cc and,skipped
R8913 T13467 T13468 punct (,3
R8914 T13468 T13469 meta 3,used
R8915 T13469 T13460 conj used,skipped
R8916 T13470 T13468 punct ),3
R8917 T13471 T13472 amod embryonic,tissue
R8918 T13472 T13473 compound tissue,sections
R8919 T13473 T13469 nsubj sections,used
R8920 T13474 T13475 det a,mix
R8921 T13475 T13469 dobj mix,used
R8922 T13476 T13475 amod different,mix
R8923 T13477 T13475 compound color,mix
R8924 T13478 T13475 compound development,mix
R8925 T13479 T13480 punct (,Thut
R8926 T13480 T13469 meta Thut,used
R8927 T13481 T13480 nmod et,Thut
R8928 T13482 T13480 nmod al.,Thut
R8929 T13483 T13480 nummod 2001,Thut
R8930 T13484 T13480 punct ),Thut
R8931 T13485 T13469 punct .,used
R8932 T13487 T13488 nsubjpass Probes,published
R8933 T13489 T13487 prep for,Probes
R8934 T13490 T13491 det the,genes
R8935 T13491 T13489 pobj genes,for
R8936 T13492 T13491 amod following,genes
R8937 T13493 T13488 aux have,published
R8938 T13494 T13488 auxpass been,published
R8939 T13495 T13488 advmod previously,published
R8940 T13496 T13488 punct : ,published
R8941 T13497 T13488 dobj Bmpr1a,published
R8942 T13498 T13499 punct (,Mishina
R8943 T13499 T13497 meta Mishina,Bmpr1a
R8944 T13500 T13499 nmod et,Mishina
R8945 T13501 T13499 nmod al.,Mishina
R8946 T13502 T13499 nummod 1995,Mishina
R8947 T13503 T13499 punct ),Mishina
R8948 T13504 T13497 punct ", ",Bmpr1a
R8949 T13505 T13497 conj Col2a1,Bmpr1a
R8950 T13506 T13507 punct (,Metsaranta
R8951 T13507 T13505 meta Metsaranta,Col2a1
R8952 T13508 T13507 nmod et,Metsaranta
R8953 T13509 T13507 nmod al.,Metsaranta
R8954 T13510 T13507 nummod 1991,Metsaranta
R8955 T13511 T13507 punct ),Metsaranta
R8956 T13512 T13505 punct ", ",Col2a1
R8957 T13513 T13505 conj Col10a1,Col2a1
R8958 T13514 T13515 punct (,Apte
R8959 T13515 T13513 meta Apte,Col10a1
R8960 T13516 T13515 nmod et,Apte
R8961 T13517 T13515 nmod al.,Apte
R8962 T13518 T13515 nummod 1992,Apte
R8963 T13519 T13515 punct ),Apte
R8964 T13520 T13513 punct ", ",Col10a1
R8965 T13521 T13513 conj Gdf5,Col10a1
R8966 T13522 T13523 punct (,Storm
R8967 T13523 T13521 meta Storm,Gdf5
R8968 T13524 T13523 cc and,Storm
R8969 T13525 T13523 conj Kingsley,Storm
R8970 T13526 T13525 nummod 1996,Kingsley
R8971 T13527 T13525 punct ),Kingsley
R8972 T13528 T13521 punct ", ",Gdf5
R8973 T13529 T13521 conj Osteocalcin,Gdf5
R8974 T13530 T13531 punct (,Celeste
R8975 T13531 T13529 meta Celeste,Osteocalcin
R8976 T13532 T13531 nmod et,Celeste
R8977 T13533 T13531 nmod al.,Celeste
R8978 T13534 T13531 nummod 1986,Celeste
R8979 T13535 T13531 punct ),Celeste
R8980 T13536 T13529 punct ", ",Osteocalcin
R8981 T13537 T13529 cc and,Osteocalcin
R8982 T13538 T13529 conj Sox5,Osteocalcin
R8983 T13539 T13538 cc and,Sox5
R8984 T13540 T13538 conj Sox6,Sox5
R8985 T13541 T13542 punct (,Lefebvre
R8986 T13542 T13540 meta Lefebvre,Sox6
R8987 T13543 T13542 nmod et,Lefebvre
R8988 T13544 T13542 nmod al.,Lefebvre
R8989 T13545 T13542 nummod 1998,Lefebvre
R8990 T13546 T13542 punct ),Lefebvre
R8991 T13547 T13488 punct .,published
R8992 T13549 T13550 det The,templates
R8993 T13550 T13553 nsubj templates,were
R8994 T13551 T13550 amod following,templates
R8995 T13552 T13550 compound probe,templates
R8996 T13553 T13554 ccomp were,made
R8997 T13555 T13553 attr gifts,were
R8998 T13556 T13553 punct : ,were
R8999 T13557 T13553 dobj Agg,were
R9000 T13558 T13557 punct ", ",Agg
R9001 T13559 T13560 compound Dr.,Rosen
R9002 T13560 T13557 npadvmod Rosen,Agg
R9003 T13561 T13560 compound Vicki,Rosen
R9004 T13562 T13557 punct ", ",Agg
R9005 T13563 T13564 compound Genetics,Institute
R9006 T13564 T13557 npadvmod Institute,Agg
R9007 T13565 T13557 punct ;,Agg
R9008 T13566 T13557 appos Bmp2,Agg
R9009 T13567 T13566 cc and,Bmp2
R9010 T13568 T13566 conj Bmp4,Bmp2
R9011 T13569 T13566 punct ", ",Bmp2
R9012 T13570 T13571 compound Arend,Sidow
R9013 T13571 T13566 npadvmod Sidow,Bmp2
R9014 T13572 T13566 punct ", ",Bmp2
R9015 T13573 T13574 compound Stanford,University
R9016 T13574 T13566 npadvmod University,Bmp2
R9017 T13575 T13557 punct ;,Agg
R9018 T13576 T13557 appos Col1a1,Agg
R9019 T13577 T13576 punct ", ",Col1a1
R9020 T13578 T13579 compound Bjorn,Olsen
R9021 T13579 T13576 npadvmod Olsen,Col1a1
R9022 T13580 T13576 punct ", ",Col1a1
R9023 T13581 T13582 compound Harvard,School
R9024 T13582 T13576 npadvmod School,Col1a1
R9025 T13583 T13582 compound Medical,School
R9026 T13584 T13554 punct ;,made
R9027 T13585 T13586 nmod Bmpr1b,probes
R9028 T13586 T13554 nsubjpass probes,made
R9029 T13587 T13585 punct ", ",Bmpr1b
R9030 T13588 T13585 conj Col3a1,Bmpr1b
R9031 T13589 T13588 punct ", ",Col3a1
R9032 T13590 T13588 cc and,Col3a1
R9033 T13591 T13588 conj Mat4,Col3a1
R9034 T13592 T13554 auxpass were,made
R9035 T13593 T13554 prep from,made
R9036 T13594 T13593 pobj ESTs,from
R9037 T13595 T13594 prep with,ESTs
R9038 T13596 T13597 compound IMAGE,numbers
R9039 T13597 T13595 pobj numbers,with
R9040 T13598 T13597 compound clone,numbers
R9041 T13599 T13597 appos 5056341,numbers
R9042 T13600 T13599 punct ", ",5056341
R9043 T13601 T13599 conj 478480,5056341
R9044 T13602 T13601 punct ", ",478480
R9045 T13603 T13601 cc and,478480
R9046 T13604 T13601 conj 406027,478480
R9047 T13605 T13554 punct ", ",made
R9048 T13606 T13554 advmod respectively,made
R9049 T13607 T13608 punct (,Invitrogen
R9050 T13608 T13554 parataxis Invitrogen,made
R9051 T13609 T13608 punct ", ",Invitrogen
R9052 T13610 T13608 npadvmod Carlsbad,Invitrogen
R9053 T13611 T13608 punct ", ",Invitrogen
R9054 T13612 T13608 npadvmod California,Invitrogen
R9055 T13613 T13608 punct ", ",Invitrogen
R9056 T13614 T13615 compound United,States
R9057 T13615 T13608 npadvmod States,Invitrogen
R9058 T13616 T13608 punct ),Invitrogen
R9059 T13617 T13554 punct .,made
R9060 T13619 T13620 nsubjpass Sections,fixed
R9061 T13621 T13619 prep for,Sections
R9062 T13622 T13621 pobj immunohistochemistry,for
R9063 T13623 T13620 auxpass were,fixed
R9064 T13624 T13620 prep in,fixed
R9065 T13625 T13626 nummod 4,%
R9066 T13626 T13627 compound %,PFA
R9067 T13627 T13624 pobj PFA,in
R9068 T13628 T13620 punct ", ",fixed
R9069 T13629 T13630 advmod then,digested
R9070 T13630 T13620 dep digested,fixed
R9071 T13631 T13630 prep with,digested
R9072 T13632 T13633 quantmod 942,"2,000"
R9073 T13633 T13635 nummod "2,000",U
R9074 T13634 T13633 punct –,"2,000"
R9075 T13635 T13636 nmod U,hyaluronindase
R9076 T13636 T13631 pobj hyaluronindase,with
R9077 T13637 T13638 punct /,ml
R9078 T13638 T13635 prep ml,U
R9079 T13639 T13640 nmod type,S
R9080 T13640 T13636 nmod S,hyaluronindase
R9081 T13641 T13640 nummod IV,S
R9082 T13642 T13640 punct -,S
R9083 T13643 T13636 amod bovine,hyaluronindase
R9084 T13644 T13645 punct (,Sigma
R9085 T13645 T13636 parataxis Sigma,hyaluronindase
R9086 T13646 T13645 punct ", ",Sigma
R9087 T13647 T13648 compound St.,Louis
R9088 T13648 T13645 npadvmod Louis,Sigma
R9089 T13649 T13645 punct ", ",Sigma
R9090 T13650 T13645 npadvmod Missouri,Sigma
R9091 T13651 T13645 punct ", ",Sigma
R9092 T13652 T13653 compound United,States
R9093 T13653 T13645 npadvmod States,Sigma
R9094 T13654 T13645 punct ),Sigma
R9095 T13655 T13630 prep in,digested
R9096 T13656 T13655 pobj PBS,in
R9097 T13657 T13656 punct (,PBS
R9098 T13658 T13656 npadvmod pH,PBS
R9099 T13659 T13658 nummod 5,pH
R9100 T13660 T13656 punct ),PBS
R9101 T13661 T13630 prep at,digested
R9102 T13662 T13663 nummod 37,°C
R9103 T13663 T13661 pobj °C,at
R9104 T13664 T13630 prep for,digested
R9105 T13665 T13666 nummod 30,min
R9106 T13666 T13664 pobj min,for
R9107 T13667 T13666 prep to,min
R9108 T13668 T13669 nummod 2,h
R9109 T13669 T13667 pobj h,to
R9110 T13670 T13666 prep depending,min
R9111 T13671 T13670 prep on,depending
R9112 T13672 T13673 det the,stage
R9113 T13673 T13671 pobj stage,on
R9114 T13674 T13620 punct .,fixed
R9115 T13676 T13677 nsubjpass Slides,washed
R9116 T13678 T13677 auxpass were,washed
R9117 T13679 T13677 advmod then,washed
R9118 T13680 T13677 prep in,washed
R9119 T13681 T13680 pobj PBS,in
R9120 T13682 T13677 punct ", ",washed
R9121 T13683 T13677 conj treated,washed
R9122 T13684 T13683 prep with,treated
R9123 T13685 T13686 nummod 0.3,%
R9124 T13686 T13687 compound %,peroxide
R9125 T13687 T13684 pobj peroxide,with
R9126 T13688 T13687 compound hydrogen,peroxide
R9127 T13689 T13683 prep in,treated
R9128 T13690 T13691 nummod 100,%
R9129 T13691 T13692 compound %,methanol
R9130 T13692 T13689 pobj methanol,in
R9131 T13693 T13683 prep for,treated
R9132 T13694 T13695 nummod 30,min
R9133 T13695 T13693 pobj min,for
R9134 T13696 T13683 punct ", ",treated
R9135 T13697 T13683 conj washed,treated
R9136 T13698 T13697 punct ", ",washed
R9137 T13699 T13697 conj blocked,washed
R9138 T13700 T13699 prep with,blocked
R9139 T13701 T13700 pobj PBS,with
R9140 T13702 T13701 punct +,PBS
R9141 T13703 T13704 nummod 0.05,%
R9142 T13704 T13705 compound %,Tween20
R9143 T13705 T13701 appos Tween20,PBS
R9144 T13706 T13701 punct +,PBS
R9145 T13707 T13708 nummod 5,%
R9146 T13708 T13709 nmod %,serum
R9147 T13709 T13701 appos serum,PBS
R9148 T13710 T13709 nmod goat,serum
R9149 T13711 T13710 cc or,goat
R9150 T13712 T13713 amod fetal,bovine
R9151 T13713 T13710 conj bovine,goat
R9152 T13714 T13699 punct ", ",blocked
R9153 T13715 T13699 conj washed,blocked
R9154 T13716 T13715 advmod again,washed
R9155 T13717 T13715 punct ", ",washed
R9156 T13718 T13715 cc and,washed
R9157 T13719 T13715 conj incubated,washed
R9158 T13720 T13719 prep with,incubated
R9159 T13721 T13722 amod primary,antibodies
R9160 T13722 T13720 pobj antibodies,with
R9161 T13723 T13719 prep in,incubated
R9162 T13724 T13723 pobj PBS,in
R9163 T13725 T13724 punct +,PBS
R9164 T13726 T13727 nummod 0.05,%
R9165 T13727 T13728 compound %,Tween
R9166 T13728 T13724 appos Tween,PBS
R9167 T13729 T13728 nummod 20,Tween
R9168 T13730 T13724 punct +,PBS
R9169 T13731 T13732 nummod 1,%
R9170 T13732 T13733 nmod %,serum
R9171 T13733 T13724 appos serum,PBS
R9172 T13734 T13733 nmod goat,serum
R9173 T13735 T13734 cc or,goat
R9174 T13736 T13737 amod fetal,bovine
R9175 T13737 T13734 conj bovine,goat
R9176 T13738 T13719 advmod overnight,incubated
R9177 T13739 T13719 prep at,incubated
R9178 T13740 T13741 nummod 4,°C
R9179 T13741 T13739 pobj °C,at
R9180 T13742 T13677 punct .,washed
R9181 T13744 T13745 npadvmod Biotin,labeled
R9182 T13745 T13747 amod labeled,antibodies
R9183 T13746 T13745 punct -,labeled
R9184 T13747 T13749 nsubjpass antibodies,tagged
R9185 T13748 T13747 amod secondary,antibodies
R9186 T13750 T13751 punct (,Labs
R9187 T13751 T13747 parataxis Labs,antibodies
R9188 T13752 T13751 compound Vector,Labs
R9189 T13753 T13751 punct ),Labs
R9190 T13754 T13749 auxpass were,tagged
R9191 T13755 T13749 prep with,tagged
R9192 T13756 T13755 pobj HRP,with
R9193 T13757 T13749 advcl using,tagged
R9194 T13758 T13759 det the,kit
R9195 T13759 T13757 dobj kit,using
R9196 T13760 T13761 compound Vectastain,Elite
R9197 T13761 T13759 compound Elite,kit
R9198 T13762 T13759 compound ABC,kit
R9199 T13763 T13764 punct (,Labs
R9200 T13764 T13759 parataxis Labs,kit
R9201 T13765 T13764 compound Vector,Labs
R9202 T13766 T13764 punct ),Labs
R9203 T13767 T13749 advcl followed,tagged
R9204 T13768 T13767 agent by,followed
R9205 T13769 T13768 pobj detection,by
R9206 T13770 T13769 prep with,detection
R9207 T13771 T13770 pobj DAB,with
R9208 T13772 T13773 punct (,Labs
R9209 T13773 T13771 parataxis Labs,DAB
R9210 T13774 T13773 compound Vector,Labs
R9211 T13775 T13773 punct ),Labs
R9212 T13776 T13749 punct .,tagged
R9213 T13778 T13779 amod Primary,antibodies
R9214 T13779 T13780 nsubj antibodies,were
R9215 T13781 T13779 cc and,antibodies
R9216 T13782 T13779 conj dilutions,antibodies
R9217 T13783 T13779 acl used,antibodies
R9218 T13784 T13780 punct : ,were
R9219 T13785 T13786 nmod goat,MMP13
R9220 T13786 T13780 attr MMP13,were
R9221 T13787 T13785 amod anti-mouse,goat
R9222 T13788 T13786 punct ", ",MMP13
R9223 T13789 T13786 npadvmod 1,MMP13
R9224 T13790 T13791 punct :,100
R9225 T13791 T13789 prep 100,1
R9226 T13792 T13793 punct (,International
R9227 T13793 T13789 parataxis International,1
R9228 T13794 T13793 compound Chemicon,International
R9229 T13795 T13793 punct ", ",International
R9230 T13796 T13793 npadvmod Temecula,International
R9231 T13797 T13793 punct ", ",International
R9232 T13798 T13793 npadvmod California,International
R9233 T13799 T13793 punct ", ",International
R9234 T13800 T13801 compound United,States
R9235 T13801 T13793 npadvmod States,International
R9236 T13802 T13793 punct ),International
R9237 T13803 T13786 punct ;,MMP13
R9238 T13804 T13805 nmod rabbit,SOX9
R9239 T13805 T13786 appos SOX9,MMP13
R9240 T13806 T13804 amod anti-human,rabbit
R9241 T13807 T13805 punct ", ",SOX9
R9242 T13808 T13805 npadvmod 1,SOX9
R9243 T13809 T13810 punct :,500
R9244 T13810 T13808 prep 500,1
R9245 T13811 T13812 punct (,Morais
R9246 T13812 T13808 meta Morais,1
R9247 T13813 T13812 nmod da,Morais
R9248 T13814 T13812 nmod Silva,Morais
R9249 T13815 T13812 nmod et,Morais
R9250 T13816 T13812 nmod al.,Morais
R9251 T13817 T13812 nummod 1996,Morais
R9252 T13818 T13812 punct ),Morais
R9253 T13819 T13786 punct ;,MMP13
R9254 T13820 T13821 nmod rabbit,SOX9
R9255 T13821 T13786 appos SOX9,MMP13
R9256 T13822 T13821 amod anti-phosphorylated,SOX9
R9257 T13823 T13821 punct -,SOX9
R9258 T13824 T13821 punct (,SOX9
R9259 T13825 T13826 nmod SOX9,P
R9260 T13826 T13821 appos P,SOX9
R9261 T13827 T13826 punct .,P
R9262 T13828 T13821 punct ),SOX9
R9263 T13829 T13821 punct ", ",SOX9
R9264 T13830 T13821 npadvmod 1,SOX9
R9265 T13831 T13832 punct :,10
R9266 T13832 T13830 prep 10,1
R9267 T13833 T13834 punct –,1
R9268 T13834 T13830 prep 1,1
R9269 T13835 T13836 punct :,250
R9270 T13836 T13834 prep 250,1
R9271 T13837 T13838 punct (,Huang
R9272 T13838 T13780 meta Huang,were
R9273 T13839 T13838 nmod et,Huang
R9274 T13840 T13838 nmod al.,Huang
R9275 T13841 T13838 nummod 2000,Huang
R9276 T13842 T13838 punct ),Huang
R9277 T13843 T13780 punct .,were