PMC:516044 / 5290-6994 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1678 0-8 JJ denotes Confocal
T1679 9-28 NN denotes immunocytochemistry
T1680 28-221 sentence denotes Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.) and transcardially perfused with a Cl--free phosphate-buffered Na-gluconate solution containing 4% paraformaldehyde.
T1681 29-36 NNS denotes Animals
T1683 37-41 VBD denotes were
T1684 42-48 RB denotes deeply
T1682 49-61 VBN denotes anesthetized
T1685 62-66 IN denotes with
T1686 67-73 NN denotes sodium
T1687 74-87 NN denotes pentobarbital
T1688 88-89 -LRB- denotes (
T1690 89-92 CD denotes 100
T1689 93-95 NNS denotes mg
T1691 95-96 SYM denotes /
T1692 96-98 NN denotes kg
T1693 99-103 RB denotes i.p.
T1694 103-104 -RRB- denotes )
T1695 105-108 CC denotes and
T1696 109-123 RB denotes transcardially
T1697 124-132 VBN denotes perfused
T1698 133-137 IN denotes with
T1699 138-139 DT denotes a
T1701 140-142 NN denotes Cl
T1703 142-143 SYM denotes -
T1704 143-144 HYPH denotes -
T1702 144-148 JJ denotes free
T1705 149-158 NN denotes phosphate
T1707 158-159 HYPH denotes -
T1706 159-167 VBN denotes buffered
T1708 168-170 NN denotes Na
T1710 170-171 SYM denotes -
T1709 171-180 NN denotes gluconate
T1700 181-189 NN denotes solution
T1711 190-200 VBG denotes containing
T1712 201-202 CD denotes 4
T1713 202-203 NN denotes %
T1714 204-220 NN denotes paraformaldehyde
T1715 220-221 . denotes .
T1716 221-396 sentence denotes Temporal bones were removed and cochleae fixed by perilymphatic perfusion, decalcified in EDTA, processed through a sucrose gradient and infiltrated with polyethylene glycol.
T1717 222-230 JJ denotes Temporal
T1718 231-236 NNS denotes bones
T1720 237-241 VBD denotes were
T1719 242-249 VBN denotes removed
T1721 250-253 CC denotes and
T1722 254-262 NNS denotes cochleae
T1723 263-268 VBN denotes fixed
T1724 269-271 IN denotes by
T1725 272-285 JJ denotes perilymphatic
T1726 286-295 NN denotes perfusion
T1727 295-297 , denotes ,
T1728 297-308 VBN denotes decalcified
T1729 309-311 IN denotes in
T1730 312-316 NN denotes EDTA
T1731 316-318 , denotes ,
T1732 318-327 VBN denotes processed
T1733 328-335 IN denotes through
T1734 336-337 DT denotes a
T1736 338-345 NN denotes sucrose
T1735 346-354 NN denotes gradient
T1737 355-358 CC denotes and
T1738 359-370 VBN denotes infiltrated
T1739 371-375 IN denotes with
T1740 376-388 NN denotes polyethylene
T1741 389-395 NN denotes glycol
T1742 395-396 . denotes .
T1743 396-543 sentence denotes Mid-modiolar cryosections (12 μm, CM3050S, Leica, Nussloch, Germany) were blocked in PBS with 0.2% Triton-X (PBS-TX) and 10% bovine serum albumin.
T1744 397-409 JJ denotes Mid-modiolar
T1745 410-422 NNS denotes cryosections
T1747 423-424 -LRB- denotes (
T1749 424-426 CD denotes 12
T1750 427-429 NNS denotes μm
T1751 429-431 , denotes ,
T1748 431-438 NN denotes CM3050S
T1752 438-440 , denotes ,
T1753 440-445 NNP denotes Leica
T1754 445-447 , denotes ,
T1755 447-455 NNP denotes Nussloch
T1756 455-457 , denotes ,
T1757 457-464 NNP denotes Germany
T1758 464-465 -RRB- denotes )
T1759 466-470 VBD denotes were
T1746 471-478 VBN denotes blocked
T1760 479-481 IN denotes in
T1761 482-485 NN denotes PBS
T1762 486-490 IN denotes with
T1763 491-494 CD denotes 0.2
T1764 494-495 NN denotes %
T1766 496-502 NN denotes Triton
T1767 502-503 HYPH denotes -
T1765 503-504 NN denotes X
T1768 505-506 -LRB- denotes (
T1770 506-509 NN denotes PBS
T1771 509-510 HYPH denotes -
T1769 510-512 NN denotes TX
T1772 512-513 -RRB- denotes )
T1773 514-517 CC denotes and
T1774 518-520 CD denotes 10
T1775 520-521 NN denotes %
T1777 522-528 JJ denotes bovine
T1778 529-534 NN denotes serum
T1776 535-542 NN denotes albumin
T1779 542-543 . denotes .
T1780 543-984 sentence denotes Slides were incubated overnight at 4°C with primary antibodies in PBS-TX with 1–3% BSA [rabbit anti-pendrin, 1:500 (h766–780); rat anti-ZO-1, 1:100 (Chemicon, Temecula, CA); goat anti-KCNQ1, 1:200 (C20, Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-KCNE1, 1:200 (Alomone, Jerusalem, Israel); rabbit anti-KCNJ10, 1:300 (Alomone); rabbit anti-SLC12A2, 1:100 (Chemicon); and rabbit anti-connexin 26, 1:100 (Zymed, San Francisco, CA)].
T1781 544-550 NNS denotes Slides
T1783 551-555 VBD denotes were
T1782 556-565 VBN denotes incubated
T1784 566-575 RB denotes overnight
T1785 576-578 IN denotes at
T1786 579-580 CD denotes 4
T1787 580-582 NN denotes °C
T1788 583-587 IN denotes with
T1789 588-595 JJ denotes primary
T1790 596-606 NNS denotes antibodies
T1791 607-609 IN denotes in
T1792 610-613 NN denotes PBS
T1794 613-614 HYPH denotes -
T1793 614-616 NN denotes TX
T1795 617-621 IN denotes with
T1796 622-623 CD denotes 1
T1798 623-624 SYM denotes
T1797 624-625 CD denotes 3
T1799 625-626 NN denotes %
T1800 627-630 NN denotes BSA
T1801 631-632 -LRB- denotes [
T1803 632-638 NN denotes rabbit
T1804 639-651 NN denotes anti-pendrin
T1805 651-653 , denotes ,
T1806 653-654 CD denotes 1
T1807 654-655 SYM denotes :
T1808 655-658 CD denotes 500
T1809 659-660 -LRB- denotes (
T1802 660-664 NN denotes h766
T1810 664-665 SYM denotes
T1811 665-668 CD denotes 780
T1812 668-669 -RRB- denotes )
T1813 669-670 , denotes ;
T1814 671-674 NN denotes rat
T1815 675-682 NN denotes anti-ZO
T1817 682-683 HYPH denotes -
T1818 683-684 CD denotes 1
T1819 684-686 , denotes ,
T1820 686-687 CD denotes 1
T1821 687-688 SYM denotes :
T1822 688-691 CD denotes 100
T1823 692-693 -LRB- denotes (
T1816 693-701 NNP denotes Chemicon
T1824 701-703 , denotes ,
T1825 703-711 NNP denotes Temecula
T1826 711-713 , denotes ,
T1827 713-715 NNP denotes CA
T1828 715-716 -RRB- denotes )
T1829 716-717 , denotes ;
T1830 718-722 NN denotes goat
T1831 723-733 NN denotes anti-KCNQ1
T1833 733-735 , denotes ,
T1834 735-736 CD denotes 1
T1835 736-737 SYM denotes :
T1836 737-740 CD denotes 200
T1837 741-742 -LRB- denotes (
T1832 742-745 NN denotes C20
T1838 745-747 , denotes ,
T1839 747-752 NNP denotes Santa
T1840 753-757 NNP denotes Cruz
T1841 758-771 NNP denotes Biotechnology
T1842 771-773 , denotes ,
T1843 773-778 NNP denotes Santa
T1844 779-783 NNP denotes Cruz
T1845 783-785 , denotes ,
T1846 785-787 NNP denotes CA
T1847 787-788 -RRB- denotes )
T1848 788-790 , denotes ,
T1849 790-796 NN denotes rabbit
T1850 797-807 NN denotes anti-KCNE1
T1852 807-809 , denotes ,
T1853 809-810 CD denotes 1
T1854 810-811 SYM denotes :
T1855 811-814 CD denotes 200
T1856 815-816 -LRB- denotes (
T1851 816-823 NNP denotes Alomone
T1857 823-825 , denotes ,
T1858 825-834 NNP denotes Jerusalem
T1859 834-836 , denotes ,
T1860 836-842 NNP denotes Israel
T1861 842-843 -RRB- denotes )
T1862 843-844 , denotes ;
T1863 845-851 NN denotes rabbit
T1864 852-863 NN denotes anti-KCNJ10
T1866 863-865 , denotes ,
T1867 865-866 CD denotes 1
T1868 866-867 SYM denotes :
T1869 867-870 CD denotes 300
T1870 871-872 -LRB- denotes (
T1865 872-879 NNP denotes Alomone
T1871 879-880 -RRB- denotes )
T1872 880-881 , denotes ;
T1873 882-888 NN denotes rabbit
T1874 889-901 NN denotes anti-SLC12A2
T1876 901-903 , denotes ,
T1877 903-904 CD denotes 1
T1878 904-905 SYM denotes :
T1879 905-908 CD denotes 100
T1880 909-910 -LRB- denotes (
T1875 910-918 NNP denotes Chemicon
T1881 918-919 -RRB- denotes )
T1882 919-920 : denotes ;
T1883 921-924 CC denotes and
T1884 925-931 NN denotes rabbit
T1885 932-945 NN denotes anti-connexin
T1887 946-948 CD denotes 26
T1888 948-950 , denotes ,
T1889 950-951 CD denotes 1
T1890 951-952 SYM denotes :
T1891 952-955 CD denotes 100
T1892 956-957 -LRB- denotes (
T1886 957-962 NNP denotes Zymed
T1893 962-964 , denotes ,
T1894 964-967 NNP denotes San
T1895 968-977 NNP denotes Francisco
T1896 977-979 , denotes ,
T1897 979-981 NNP denotes CA
T1898 981-982 -RRB- denotes )
T1899 982-983 -RRB- denotes ]
T1900 983-984 . denotes .
T1901 984-1250 sentence denotes Slides were washed in PBS-TX and incubated for 1 h at 25°C with appropriate secondary antibodies at a 1:1000 dilution in PBS-TX with 1–3% BSA [donkey anti-rabbit Alexa 488, chicken anti-rat Alexa 594, and chicken anti-goat Alexa 594 (Molecular Probes, Eugene, OR)].
T1902 985-991 NNS denotes Slides
T1904 992-996 VBD denotes were
T1903 997-1003 VBN denotes washed
T1905 1004-1006 IN denotes in
T1906 1007-1010 NN denotes PBS
T1908 1010-1011 HYPH denotes -
T1907 1011-1013 NN denotes TX
T1909 1014-1017 CC denotes and
T1910 1018-1027 VBN denotes incubated
T1911 1028-1031 IN denotes for
T1912 1032-1033 CD denotes 1
T1913 1034-1035 NN denotes h
T1914 1036-1038 IN denotes at
T1915 1039-1041 CD denotes 25
T1916 1041-1043 NN denotes °C
T1917 1044-1048 IN denotes with
T1918 1049-1060 JJ denotes appropriate
T1920 1061-1070 JJ denotes secondary
T1919 1071-1081 NNS denotes antibodies
T1921 1082-1084 IN denotes at
T1922 1085-1086 DT denotes a
T1924 1087-1088 CD denotes 1
T1925 1088-1089 SYM denotes :
T1926 1089-1093 CD denotes 1000
T1923 1094-1102 NN denotes dilution
T1927 1103-1105 IN denotes in
T1928 1106-1109 NN denotes PBS
T1930 1109-1110 HYPH denotes -
T1929 1110-1112 NN denotes TX
T1931 1113-1117 IN denotes with
T1932 1118-1119 CD denotes 1
T1934 1119-1120 SYM denotes
T1933 1120-1121 CD denotes 3
T1935 1121-1122 NN denotes %
T1936 1123-1126 NN denotes BSA
T1937 1127-1128 -LRB- denotes [
T1939 1128-1134 NN denotes donkey
T1941 1135-1146 JJ denotes anti-rabbit
T1940 1147-1152 NNP denotes Alexa
T1942 1153-1156 CD denotes 488
T1943 1156-1158 , denotes ,
T1944 1158-1165 NN denotes chicken
T1946 1166-1174 JJ denotes anti-rat
T1945 1175-1180 NNP denotes Alexa
T1947 1181-1184 CD denotes 594
T1948 1184-1186 , denotes ,
T1949 1186-1189 CC denotes and
T1950 1190-1197 NN denotes chicken
T1952 1198-1207 JJ denotes anti-goat
T1951 1208-1213 NNP denotes Alexa
T1953 1214-1217 CD denotes 594
T1954 1218-1219 -LRB- denotes (
T1955 1219-1228 NNP denotes Molecular
T1938 1229-1235 NNP denotes Probes
T1956 1235-1237 , denotes ,
T1957 1237-1243 NNP denotes Eugene
T1958 1243-1245 , denotes ,
T1959 1245-1247 NNP denotes OR
T1960 1247-1248 -RRB- denotes )
T1961 1248-1249 -RRB- denotes ]
T1962 1249-1250 . denotes .
T1963 1250-1351 sentence denotes Actin filaments were visualized by staining with Alexa 488 conjugated phalloidin (Molecular Probes).
T1964 1251-1256 NN denotes Actin
T1965 1257-1266 NNS denotes filaments
T1967 1267-1271 VBD denotes were
T1966 1272-1282 VBN denotes visualized
T1968 1283-1285 IN denotes by
T1969 1286-1294 VBG denotes staining
T1970 1295-1299 IN denotes with
T1971 1300-1305 NNP denotes Alexa
T1973 1306-1309 CD denotes 488
T1972 1310-1320 VBN denotes conjugated
T1974 1321-1331 NN denotes phalloidin
T1975 1332-1333 -LRB- denotes (
T1977 1333-1342 NNP denotes Molecular
T1976 1343-1349 NNP denotes Probes
T1978 1349-1350 -RRB- denotes )
T1979 1350-1351 . denotes .
T1980 1351-1558 sentence denotes After incubation, slides were washed with PBS-TX, mounted with FluorSave (Calbiochem, La Jolla, CA), and viewed by confocal microscopy (LSM 5 Pascal or LSM 510 Meta, Carl Zeiss, Jena or Göttingen, Germany).
T1981 1352-1357 IN denotes After
T1983 1358-1368 NN denotes incubation
T1984 1368-1370 , denotes ,
T1985 1370-1376 NNS denotes slides
T1986 1377-1381 VBD denotes were
T1982 1382-1388 VBN denotes washed
T1987 1389-1393 IN denotes with
T1988 1394-1397 NN denotes PBS
T1990 1397-1398 HYPH denotes -
T1989 1398-1400 NN denotes TX
T1991 1400-1402 , denotes ,
T1992 1402-1409 VBN denotes mounted
T1993 1410-1414 IN denotes with
T1994 1415-1424 NNP denotes FluorSave
T1995 1425-1426 -LRB- denotes (
T1996 1426-1436 NNP denotes Calbiochem
T1997 1436-1438 , denotes ,
T1998 1438-1440 NNP denotes La
T1999 1441-1446 NNP denotes Jolla
T2000 1446-1448 , denotes ,
T2001 1448-1450 NNP denotes CA
T2002 1450-1451 -RRB- denotes )
T2003 1451-1453 , denotes ,
T2004 1453-1456 CC denotes and
T2005 1457-1463 VBN denotes viewed
T2006 1464-1466 IN denotes by
T2007 1467-1475 JJ denotes confocal
T2008 1476-1486 NN denotes microscopy
T2009 1487-1488 -LRB- denotes (
T2011 1488-1491 NN denotes LSM
T2012 1492-1493 CD denotes 5
T2010 1494-1500 NN denotes Pascal
T2013 1501-1503 CC denotes or
T2014 1504-1507 NN denotes LSM
T2016 1508-1511 CD denotes 510
T2015 1512-1516 NN denotes Meta
T2017 1516-1518 , denotes ,
T2018 1518-1522 NNP denotes Carl
T2019 1523-1528 NNP denotes Zeiss
T2020 1528-1530 , denotes ,
T2021 1530-1534 NNP denotes Jena
T2022 1535-1537 CC denotes or
T2023 1538-1547 NNP denotes Göttingen
T2024 1547-1549 , denotes ,
T2025 1549-1556 NNP denotes Germany
T2026 1556-1557 -RRB- denotes )
T2027 1557-1558 . denotes .
T2028 1558-1704 sentence denotes Laser scanning brightfield images were collected to document structural preservation, for morphometric analysis and for analysis of pigmentation.
T2029 1559-1564 NN denotes Laser
T2030 1565-1573 NN denotes scanning
T2032 1574-1585 NN denotes brightfield
T2031 1586-1592 NNS denotes images
T2034 1593-1597 VBD denotes were
T2033 1598-1607 VBN denotes collected
T2035 1608-1610 TO denotes to
T2036 1611-1619 VB denotes document
T2037 1620-1630 JJ denotes structural
T2038 1631-1643 NN denotes preservation
T2039 1643-1645 , denotes ,
T2040 1645-1648 IN denotes for
T2041 1649-1661 JJ denotes morphometric
T2042 1662-1670 NN denotes analysis
T2043 1671-1674 CC denotes and
T2044 1675-1678 IN denotes for
T2045 1679-1687 NN denotes analysis
T2046 1688-1690 IN denotes of
T2047 1691-1703 NN denotes pigmentation
T2048 1703-1704 . denotes .
R1000 T1739 T1738 prep with,infiltrated
R1001 T1740 T1741 compound polyethylene,glycol
R1002 T1741 T1739 pobj glycol,with
R1003 T1742 T1723 punct .,fixed
R1004 T1744 T1745 amod Mid-modiolar,cryosections
R1005 T1745 T1746 nsubjpass cryosections,blocked
R1006 T1747 T1748 punct (,CM3050S
R1007 T1748 T1745 parataxis CM3050S,cryosections
R1008 T1749 T1750 nummod 12,μm
R1009 T1750 T1748 nmod μm,CM3050S
R1010 T1751 T1748 punct ", ",CM3050S
R1011 T1752 T1748 punct ", ",CM3050S
R1012 T1753 T1748 npadvmod Leica,CM3050S
R1013 T1754 T1748 punct ", ",CM3050S
R1014 T1755 T1748 npadvmod Nussloch,CM3050S
R1015 T1756 T1748 punct ", ",CM3050S
R1016 T1757 T1748 npadvmod Germany,CM3050S
R1017 T1758 T1748 punct ),CM3050S
R1018 T1759 T1746 auxpass were,blocked
R1019 T1760 T1746 prep in,blocked
R1020 T1761 T1760 pobj PBS,in
R1021 T1762 T1746 prep with,blocked
R1022 T1763 T1764 nummod 0.2,%
R1023 T1764 T1765 compound %,X
R1024 T1765 T1762 pobj X,with
R1025 T1766 T1765 compound Triton,X
R1026 T1767 T1765 punct -,X
R1027 T1768 T1769 punct (,TX
R1028 T1769 T1765 parataxis TX,X
R1029 T1770 T1769 compound PBS,TX
R1030 T1771 T1769 punct -,TX
R1031 T1772 T1769 punct ),TX
R1032 T1773 T1765 cc and,X
R1033 T1774 T1775 nummod 10,%
R1034 T1775 T1776 nmod %,albumin
R1035 T1776 T1765 conj albumin,X
R1036 T1777 T1776 amod bovine,albumin
R1037 T1778 T1776 compound serum,albumin
R1038 T1779 T1746 punct .,blocked
R1039 T1781 T1782 nsubjpass Slides,incubated
R1040 T1783 T1782 auxpass were,incubated
R1041 T1784 T1782 advmod overnight,incubated
R1042 T1785 T1782 prep at,incubated
R1043 T1786 T1787 nummod 4,°C
R1044 T1787 T1785 pobj °C,at
R1045 T1788 T1782 prep with,incubated
R1046 T1789 T1790 amod primary,antibodies
R1047 T1790 T1788 pobj antibodies,with
R1048 T1791 T1782 prep in,incubated
R1049 T1792 T1793 compound PBS,TX
R1050 T1793 T1791 pobj TX,in
R1051 T1794 T1793 punct -,TX
R1052 T1795 T1782 prep with,incubated
R1053 T1796 T1797 quantmod 1,3
R1054 T1797 T1799 nummod 3,%
R1055 T1798 T1797 punct –,3
R1056 T1799 T1800 compound %,BSA
R1057 T1800 T1795 pobj BSA,with
R1058 T1801 T1802 punct [,h766
R1059 T1802 T1782 parataxis h766,incubated
R1060 T1803 T1804 compound rabbit,anti-pendrin
R1061 T1804 T1802 dep anti-pendrin,h766
R1062 T1805 T1802 punct ", ",h766
R1063 T1806 T1802 dep 1,h766
R1064 T1807 T1808 punct :,500
R1065 T1808 T1806 prep 500,1
R1066 T1809 T1802 punct (,h766
R1067 T1810 T1811 punct –,780
R1068 T1811 T1802 prep 780,h766
R1069 T1812 T1802 punct ),h766
R1070 T1813 T1802 punct ;,h766
R1071 T1814 T1815 compound rat,anti-ZO
R1072 T1815 T1816 dep anti-ZO,Chemicon
R1073 T1816 T1802 conj Chemicon,h766
R1074 T1817 T1815 punct -,anti-ZO
R1075 T1818 T1815 nummod 1,anti-ZO
R1076 T1819 T1816 punct ", ",Chemicon
R1077 T1820 T1816 dep 1,Chemicon
R1078 T1821 T1822 punct :,100
R1079 T1822 T1820 prep 100,1
R1080 T1823 T1816 punct (,Chemicon
R1081 T1824 T1816 punct ", ",Chemicon
R1082 T1825 T1816 npadvmod Temecula,Chemicon
R1083 T1826 T1816 punct ", ",Chemicon
R1084 T1827 T1816 npadvmod CA,Chemicon
R1085 T1828 T1816 punct ),Chemicon
R1086 T1829 T1816 punct ;,Chemicon
R1087 T1830 T1831 compound goat,anti-KCNQ1
R1088 T1831 T1832 dep anti-KCNQ1,C20
R1089 T1832 T1816 conj C20,Chemicon
R1090 T1833 T1832 punct ", ",C20
R1091 T1834 T1832 dep 1,C20
R1092 T1835 T1836 punct :,200
R1093 T1836 T1834 prep 200,1
R1094 T1837 T1832 punct (,C20
R1095 T1838 T1832 punct ", ",C20
R1096 T1839 T1840 compound Santa,Cruz
R1097 T1840 T1841 compound Cruz,Biotechnology
R1098 T1841 T1832 npadvmod Biotechnology,C20
R1099 T1842 T1832 punct ", ",C20
R1100 T1843 T1844 compound Santa,Cruz
R1101 T1844 T1832 npadvmod Cruz,C20
R1102 T1845 T1832 punct ", ",C20
R1103 T1846 T1832 npadvmod CA,C20
R1104 T1847 T1832 punct ),C20
R1105 T1848 T1832 punct ", ",C20
R1106 T1849 T1850 compound rabbit,anti-KCNE1
R1107 T1850 T1851 dep anti-KCNE1,Alomone
R1108 T1851 T1832 conj Alomone,C20
R1109 T1852 T1851 punct ", ",Alomone
R1110 T1853 T1851 dep 1,Alomone
R1111 T1854 T1855 punct :,200
R1112 T1855 T1853 prep 200,1
R1113 T1856 T1851 punct (,Alomone
R1114 T1857 T1851 punct ", ",Alomone
R1115 T1858 T1851 npadvmod Jerusalem,Alomone
R1116 T1859 T1851 punct ", ",Alomone
R1117 T1860 T1851 npadvmod Israel,Alomone
R1118 T1861 T1851 punct ),Alomone
R1119 T1862 T1851 punct ;,Alomone
R1120 T1863 T1864 compound rabbit,anti-KCNJ10
R1121 T1864 T1865 dep anti-KCNJ10,Alomone
R1122 T1865 T1851 conj Alomone,Alomone
R1123 T1866 T1865 punct ", ",Alomone
R1124 T1867 T1865 dep 1,Alomone
R1125 T1868 T1869 punct :,300
R1126 T1869 T1867 prep 300,1
R1127 T1870 T1865 punct (,Alomone
R1128 T1871 T1865 punct ),Alomone
R1129 T1872 T1865 punct ;,Alomone
R1130 T1873 T1874 compound rabbit,anti-SLC12A2
R1131 T1874 T1875 dep anti-SLC12A2,Chemicon
R1132 T1875 T1865 conj Chemicon,Alomone
R1133 T1876 T1875 punct ", ",Chemicon
R1134 T1877 T1875 dep 1,Chemicon
R1135 T1878 T1879 punct :,100
R1136 T1879 T1877 prep 100,1
R1137 T1880 T1875 punct (,Chemicon
R1138 T1881 T1875 punct ),Chemicon
R1139 T1882 T1875 punct ;,Chemicon
R1140 T1883 T1875 cc and,Chemicon
R1141 T1884 T1885 compound rabbit,anti-connexin
R1142 T1885 T1886 dep anti-connexin,Zymed
R1143 T1886 T1875 conj Zymed,Chemicon
R1144 T1887 T1885 nummod 26,anti-connexin
R1145 T1888 T1886 punct ", ",Zymed
R1146 T1889 T1886 dep 1,Zymed
R1147 T1890 T1891 punct :,100
R1148 T1891 T1889 prep 100,1
R1149 T1892 T1886 punct (,Zymed
R1150 T1893 T1886 punct ", ",Zymed
R1151 T1894 T1895 compound San,Francisco
R1152 T1895 T1886 npadvmod Francisco,Zymed
R1153 T1896 T1886 punct ", ",Zymed
R1154 T1897 T1886 npadvmod CA,Zymed
R1155 T1898 T1886 punct ),Zymed
R1156 T1899 T1886 punct ],Zymed
R1157 T1900 T1782 punct .,incubated
R1158 T1902 T1903 nsubjpass Slides,washed
R1159 T1904 T1903 auxpass were,washed
R1160 T1905 T1903 prep in,washed
R1161 T1906 T1907 compound PBS,TX
R1162 T1907 T1905 pobj TX,in
R1163 T1908 T1907 punct -,TX
R1164 T1909 T1903 cc and,washed
R1165 T1910 T1903 conj incubated,washed
R1166 T1911 T1910 prep for,incubated
R1167 T1912 T1913 nummod 1,h
R1168 T1913 T1911 pobj h,for
R1169 T1914 T1910 prep at,incubated
R1170 T1915 T1916 nummod 25,°C
R1171 T1916 T1914 pobj °C,at
R1172 T1917 T1910 prep with,incubated
R1173 T1918 T1919 amod appropriate,antibodies
R1174 T1919 T1917 pobj antibodies,with
R1175 T1920 T1919 amod secondary,antibodies
R1176 T1921 T1919 prep at,antibodies
R1177 T1922 T1923 det a,dilution
R1178 T1923 T1921 pobj dilution,at
R1179 T1924 T1923 nummod 1,dilution
R1180 T1925 T1926 punct :,1000
R1181 T1926 T1924 prep 1000,1
R1182 T1927 T1923 prep in,dilution
R1183 T1928 T1929 compound PBS,TX
R1184 T1929 T1927 pobj TX,in
R1185 T1930 T1929 punct -,TX
R1186 T1931 T1923 prep with,dilution
R1187 T1932 T1933 quantmod 1,3
R1188 T1933 T1935 nummod 3,%
R1189 T1934 T1933 punct –,3
R1190 T1935 T1936 compound %,BSA
R1191 T1936 T1931 pobj BSA,with
R1192 T1937 T1938 punct [,Probes
R1193 T1938 T1910 parataxis Probes,incubated
R1194 T1939 T1940 nmod donkey,Alexa
R1195 T1940 T1938 dep Alexa,Probes
R1196 T1941 T1939 amod anti-rabbit,donkey
R1197 T1942 T1940 nummod 488,Alexa
R1198 T1943 T1940 punct ", ",Alexa
R1199 T1944 T1945 nmod chicken,Alexa
R1200 T1945 T1940 conj Alexa,Alexa
R1201 T1946 T1944 amod anti-rat,chicken
R1202 T1947 T1945 nummod 594,Alexa
R1203 T1948 T1945 punct ", ",Alexa
R1204 T1949 T1945 cc and,Alexa
R1205 T1950 T1951 nmod chicken,Alexa
R1206 T1951 T1945 conj Alexa,Alexa
R1207 T1952 T1950 amod anti-goat,chicken
R1208 T1953 T1951 nummod 594,Alexa
R1209 T1954 T1938 punct (,Probes
R1210 T1955 T1938 compound Molecular,Probes
R1211 T1956 T1938 punct ", ",Probes
R1212 T1957 T1938 npadvmod Eugene,Probes
R1213 T1958 T1938 punct ", ",Probes
R1214 T1959 T1938 npadvmod OR,Probes
R1215 T1960 T1938 punct ),Probes
R1216 T1961 T1938 punct ],Probes
R1217 T1962 T1903 punct .,washed
R1218 T1964 T1965 compound Actin,filaments
R1219 T1965 T1966 nsubjpass filaments,visualized
R1220 T1967 T1966 auxpass were,visualized
R1221 T1968 T1966 prep by,visualized
R1222 T1969 T1968 pcomp staining,by
R1223 T1970 T1969 prep with,staining
R1224 T1971 T1972 npadvmod Alexa,conjugated
R1225 T1972 T1974 amod conjugated,phalloidin
R1226 T1973 T1971 nummod 488,Alexa
R1227 T1974 T1970 pobj phalloidin,with
R1228 T1975 T1976 punct (,Probes
R1229 T1976 T1974 parataxis Probes,phalloidin
R1230 T1977 T1976 compound Molecular,Probes
R1231 T1978 T1976 punct ),Probes
R1232 T1979 T1966 punct .,visualized
R1233 T1981 T1982 prep After,washed
R1234 T1983 T1981 pobj incubation,After
R1235 T1984 T1982 punct ", ",washed
R1236 T1985 T1982 nsubjpass slides,washed
R1237 T1986 T1982 auxpass were,washed
R1238 T1987 T1982 prep with,washed
R1239 T1988 T1989 compound PBS,TX
R1240 T1989 T1987 pobj TX,with
R1241 T1990 T1989 punct -,TX
R1242 T1991 T1982 punct ", ",washed
R1243 T1992 T1982 conj mounted,washed
R1244 T1993 T1992 prep with,mounted
R1245 T1994 T1993 pobj FluorSave,with
R1246 T1995 T1996 punct (,Calbiochem
R1247 T1996 T1994 parataxis Calbiochem,FluorSave
R1248 T1997 T1996 punct ", ",Calbiochem
R1249 T1998 T1999 compound La,Jolla
R1250 T1999 T1996 npadvmod Jolla,Calbiochem
R1251 T2000 T1996 punct ", ",Calbiochem
R1252 T2001 T1996 npadvmod CA,Calbiochem
R1253 T2002 T1996 punct ),Calbiochem
R1254 T2003 T1992 punct ", ",mounted
R1255 T2004 T1992 cc and,mounted
R1256 T2005 T1992 conj viewed,mounted
R1257 T2006 T2005 prep by,viewed
R1258 T2007 T2008 amod confocal,microscopy
R1259 T2008 T2006 pobj microscopy,by
R1260 T2009 T2010 punct (,Pascal
R1261 T2010 T2008 parataxis Pascal,microscopy
R1262 T2011 T2010 nmod LSM,Pascal
R1263 T2012 T2010 nummod 5,Pascal
R1264 T2013 T2010 cc or,Pascal
R1265 T2014 T2015 nmod LSM,Meta
R1266 T2015 T2010 conj Meta,Pascal
R1267 T2016 T2015 nummod 510,Meta
R1268 T2017 T2010 punct ", ",Pascal
R1269 T2018 T2019 compound Carl,Zeiss
R1270 T2019 T2010 npadvmod Zeiss,Pascal
R1271 T2020 T2010 punct ", ",Pascal
R1272 T2021 T2010 npadvmod Jena,Pascal
R1273 T2022 T2021 cc or,Jena
R1274 T2023 T2021 conj Göttingen,Jena
R1275 T2024 T2010 punct ", ",Pascal
R1276 T2025 T2010 npadvmod Germany,Pascal
R1277 T2026 T2010 punct ),Pascal
R1278 T2027 T1982 punct .,washed
R1279 T2029 T2030 compound Laser,scanning
R1280 T2030 T2031 compound scanning,images
R1281 T2031 T2033 nsubjpass images,collected
R1282 T2032 T2031 compound brightfield,images
R1283 T2034 T2033 auxpass were,collected
R1284 T2035 T2036 aux to,document
R1285 T2036 T2033 advcl document,collected
R1286 T2037 T2038 amod structural,preservation
R1287 T2038 T2036 dobj preservation,document
R1288 T2039 T2033 punct ", ",collected
R1289 T2040 T2033 prep for,collected
R1290 T2041 T2042 amod morphometric,analysis
R1291 T2042 T2040 pobj analysis,for
R1292 T2043 T2040 cc and,for
R1293 T2044 T2040 conj for,for
R1294 T2045 T2044 pobj analysis,for
R1295 T2046 T2045 prep of,analysis
R1296 T2047 T2046 pobj pigmentation,of
R1297 T2048 T2033 punct .,collected
R944 T1678 T1679 amod Confocal,immunocytochemistry
R945 T1681 T1682 nsubjpass Animals,anesthetized
R946 T1683 T1682 auxpass were,anesthetized
R947 T1684 T1682 advmod deeply,anesthetized
R948 T1685 T1682 prep with,anesthetized
R949 T1686 T1687 compound sodium,pentobarbital
R950 T1687 T1685 pobj pentobarbital,with
R951 T1688 T1689 punct (,mg
R952 T1689 T1687 parataxis mg,pentobarbital
R953 T1690 T1689 nummod 100,mg
R954 T1691 T1692 punct /,kg
R955 T1692 T1689 prep kg,mg
R956 T1693 T1689 advmod i.p.,mg
R957 T1694 T1689 punct ),mg
R958 T1695 T1682 cc and,anesthetized
R959 T1696 T1697 advmod transcardially,perfused
R960 T1697 T1682 conj perfused,anesthetized
R961 T1698 T1697 prep with,perfused
R962 T1699 T1700 det a,solution
R963 T1700 T1698 pobj solution,with
R964 T1701 T1702 npadvmod Cl,free
R965 T1702 T1700 amod free,solution
R966 T1703 T1701 punct -,Cl
R967 T1704 T1702 punct -,free
R968 T1705 T1706 npadvmod phosphate,buffered
R969 T1706 T1700 amod buffered,solution
R970 T1707 T1706 punct -,buffered
R971 T1708 T1709 nmod Na,gluconate
R972 T1709 T1700 compound gluconate,solution
R973 T1710 T1708 punct -,Na
R974 T1711 T1700 acl containing,solution
R975 T1712 T1713 nummod 4,%
R976 T1713 T1714 compound %,paraformaldehyde
R977 T1714 T1711 dobj paraformaldehyde,containing
R978 T1715 T1682 punct .,anesthetized
R979 T1717 T1718 amod Temporal,bones
R980 T1718 T1719 nsubjpass bones,removed
R981 T1720 T1719 auxpass were,removed
R982 T1721 T1719 cc and,removed
R983 T1722 T1723 nsubj cochleae,fixed
R984 T1723 T1719 conj fixed,removed
R985 T1724 T1723 prep by,fixed
R986 T1725 T1726 amod perilymphatic,perfusion
R987 T1726 T1724 pobj perfusion,by
R988 T1727 T1723 punct ", ",fixed
R989 T1728 T1723 conj decalcified,fixed
R990 T1729 T1728 prep in,decalcified
R991 T1730 T1729 pobj EDTA,in
R992 T1731 T1728 punct ", ",decalcified
R993 T1732 T1728 conj processed,decalcified
R994 T1733 T1732 prep through,processed
R995 T1734 T1735 det a,gradient
R996 T1735 T1733 pobj gradient,through
R997 T1736 T1735 compound sucrose,gradient
R998 T1737 T1732 cc and,processed
R999 T1738 T1732 conj infiltrated,processed