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PMC:516044 / 2015-33345 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T770 11-18 NNP denotes Pendred
T771 19-27 NN denotes syndrome
T772 28-30 VBZ denotes is
T773 31-32 DT denotes a
T775 33-43 RB denotes relatively
T776 44-50 JJ denotes common
T777 51-60 JJ denotes autosomal
T779 60-61 HYPH denotes -
T778 61-70 JJ denotes recessive
T774 71-79 NN denotes disorder
T780 80-93 VBN denotes characterized
T781 94-96 IN denotes by
T782 97-105 NN denotes deafness
T783 106-109 CC denotes and
T784 110-116 NN denotes goiter
T785 117-118 -LRB- denotes [
T786 118-119 CD denotes 1
T787 119-120 -RRB- denotes ]
T788 120-121 . denotes .
T789 121-223 sentence denotes The syndrome is caused by mutations of the PDS gene SLC26A4, which codes for the protein pendrin [2].
T790 122-125 DT denotes The
T791 126-134 NN denotes syndrome
T793 135-137 VBZ denotes is
T792 138-144 VBN denotes caused
T794 145-147 IN denotes by
T795 148-157 NNS denotes mutations
T796 158-160 IN denotes of
T797 161-164 DT denotes the
T799 165-168 NN denotes PDS
T798 169-173 NN denotes gene
T800 174-181 NN denotes SLC26A4
T801 181-183 , denotes ,
T802 183-188 WDT denotes which
T803 189-194 VBZ denotes codes
T804 195-198 IN denotes for
T805 199-202 DT denotes the
T807 203-210 NN denotes protein
T806 211-218 NN denotes pendrin
T808 219-220 -LRB- denotes [
T809 220-221 CD denotes 2
T810 221-222 -RRB- denotes ]
T811 222-223 . denotes .
T812 223-337 sentence denotes Deafness is congenital and generally profound although sometimes late in onset and provoked by light head injury.
T813 224-232 NN denotes Deafness
T814 233-235 VBZ denotes is
T815 236-246 JJ denotes congenital
T816 247-250 CC denotes and
T817 251-260 RB denotes generally
T818 261-269 JJ denotes profound
T819 270-278 IN denotes although
T821 279-288 RB denotes sometimes
T820 289-293 JJ denotes late
T822 294-296 IN denotes in
T823 297-302 NN denotes onset
T824 303-306 CC denotes and
T825 307-315 VBN denotes provoked
T826 316-318 IN denotes by
T827 319-324 JJ denotes light
T829 325-329 NN denotes head
T828 330-336 NN denotes injury
T830 336-337 . denotes .
T831 337-373 sentence denotes Vestibular dysfunction is uncommon.
T832 338-348 JJ denotes Vestibular
T833 349-360 NN denotes dysfunction
T834 361-363 VBZ denotes is
T835 364-372 JJ denotes uncommon
T836 372-373 . denotes .
T837 373-435 sentence denotes Goiter is variable and generally develops around puberty [3].
T838 374-380 NN denotes Goiter
T839 381-383 VBZ denotes is
T840 384-392 JJ denotes variable
T841 393-396 CC denotes and
T842 397-406 RB denotes generally
T843 407-415 VBZ denotes develops
T844 416-422 IN denotes around
T845 423-430 NN denotes puberty
T846 431-432 -LRB- denotes [
T847 432-433 CD denotes 3
T848 433-434 -RRB- denotes ]
T849 434-435 . denotes .
T850 435-634 sentence denotes The cause of goiter appears to be an impairment of iodide fixation in the follicular lumen due to a reduced rate of iodide transport across the apical membrane of thyroid gland epithelial cells [4].
T851 436-439 DT denotes The
T852 440-445 NN denotes cause
T854 446-448 IN denotes of
T855 449-455 NN denotes goiter
T853 456-463 VBZ denotes appears
T856 464-466 TO denotes to
T857 467-469 VB denotes be
T858 470-472 DT denotes an
T859 473-483 NN denotes impairment
T860 484-486 IN denotes of
T861 487-493 NN denotes iodide
T862 494-502 NN denotes fixation
T863 503-505 IN denotes in
T864 506-509 DT denotes the
T866 510-520 JJ denotes follicular
T865 521-526 NN denotes lumen
T867 527-530 IN denotes due
T868 531-533 IN denotes to
T869 534-535 DT denotes a
T871 536-543 VBN denotes reduced
T870 544-548 NN denotes rate
T872 549-551 IN denotes of
T873 552-558 NN denotes iodide
T874 559-568 NN denotes transport
T875 569-575 IN denotes across
T876 576-579 DT denotes the
T878 580-586 JJ denotes apical
T877 587-595 NN denotes membrane
T879 596-598 IN denotes of
T880 599-606 JJ denotes thyroid
T881 607-612 NN denotes gland
T883 613-623 JJ denotes epithelial
T882 624-629 NNS denotes cells
T884 630-631 -LRB- denotes [
T885 631-632 CD denotes 4
T886 632-633 -RRB- denotes ]
T887 633-634 . denotes .
T888 634-779 sentence denotes A positive perchlorate discharge test and an enlarged vestibular aqueduct appear to be the most reliable clinical signs of Pendred syndrome [5].
T889 635-636 DT denotes A
T891 637-645 JJ denotes positive
T892 646-657 NN denotes perchlorate
T893 658-667 NN denotes discharge
T890 668-672 NN denotes test
T895 673-676 CC denotes and
T896 677-679 DT denotes an
T898 680-688 VBN denotes enlarged
T899 689-699 JJ denotes vestibular
T897 700-708 NN denotes aqueduct
T894 709-715 VBP denotes appear
T900 716-718 TO denotes to
T901 719-721 VB denotes be
T902 722-725 DT denotes the
T904 726-730 RBS denotes most
T905 731-739 JJ denotes reliable
T906 740-748 JJ denotes clinical
T903 749-754 NNS denotes signs
T907 755-757 IN denotes of
T908 758-765 NNP denotes Pendred
T909 766-774 NN denotes syndrome
T910 775-776 -LRB- denotes [
T911 776-777 CD denotes 5
T912 777-778 -RRB- denotes ]
T913 778-779 . denotes .
T914 779-863 sentence denotes Pendrin is an anion exchanger that can transport Cl-, I-, HCO3 - and formate [6,7].
T915 780-787 NN denotes Pendrin
T916 788-790 VBZ denotes is
T917 791-793 DT denotes an
T919 794-799 NN denotes anion
T918 800-809 NN denotes exchanger
T920 810-814 WDT denotes that
T922 815-818 MD denotes can
T921 819-828 VB denotes transport
T923 829-831 NN denotes Cl
T924 831-832 SYM denotes -
T925 832-834 , denotes ,
T926 834-835 NN denotes I
T927 835-836 SYM denotes -
T928 836-838 , denotes ,
T929 838-842 NN denotes HCO3
T930 843-844 SYM denotes -
T931 845-848 CC denotes and
T932 849-856 NN denotes formate
T933 857-858 -LRB- denotes [
T935 858-859 CD denotes 6
T936 859-860 , denotes ,
T934 860-861 CD denotes 7
T937 861-862 -RRB- denotes ]
T938 862-863 . denotes .
T939 863-991 sentence denotes Expression has been found in the inner ear and thyroid gland consistent with the clinical signs of deafness and goiter [2,3,8].
T940 864-874 NN denotes Expression
T942 875-878 VBZ denotes has
T943 879-883 VBN denotes been
T941 884-889 VBN denotes found
T944 890-892 IN denotes in
T945 893-896 DT denotes the
T947 897-902 JJ denotes inner
T946 903-906 NN denotes ear
T948 907-910 CC denotes and
T949 911-918 NN denotes thyroid
T950 919-924 NN denotes gland
T951 925-935 JJ denotes consistent
T952 936-940 IN denotes with
T953 941-944 DT denotes the
T955 945-953 JJ denotes clinical
T954 954-959 NNS denotes signs
T956 960-962 IN denotes of
T957 963-971 NN denotes deafness
T958 972-975 CC denotes and
T959 976-982 NN denotes goiter
T960 983-984 -LRB- denotes [
T962 984-985 CD denotes 2
T963 985-986 , denotes ,
T964 986-987 CD denotes 3
T965 987-988 , denotes ,
T961 988-989 CD denotes 8
T966 989-990 -RRB- denotes ]
T967 990-991 . denotes .
T968 991-1121 sentence denotes In addition, pendrin expression has been found in the kidney [9], mammary gland [10], uterus [11], testes [12] and placenta [13].
T969 992-994 IN denotes In
T971 995-1003 NN denotes addition
T972 1003-1005 , denotes ,
T973 1005-1012 NN denotes pendrin
T974 1013-1023 NN denotes expression
T975 1024-1027 VBZ denotes has
T976 1028-1032 VBN denotes been
T970 1033-1038 VBN denotes found
T977 1039-1041 IN denotes in
T978 1042-1045 DT denotes the
T979 1046-1052 NN denotes kidney
T980 1053-1054 -LRB- denotes [
T981 1054-1055 CD denotes 9
T982 1055-1056 -RRB- denotes ]
T983 1056-1058 , denotes ,
T984 1058-1065 JJ denotes mammary
T985 1066-1071 NN denotes gland
T986 1072-1073 -LRB- denotes [
T987 1073-1075 CD denotes 10
T988 1075-1076 -RRB- denotes ]
T989 1076-1078 , denotes ,
T990 1078-1084 NN denotes uterus
T991 1085-1086 -LRB- denotes [
T992 1086-1088 CD denotes 11
T993 1088-1089 -RRB- denotes ]
T994 1089-1091 , denotes ,
T995 1091-1097 NNS denotes testes
T996 1098-1099 -LRB- denotes [
T997 1099-1101 CD denotes 12
T998 1101-1102 -RRB- denotes ]
T999 1103-1106 CC denotes and
T1000 1107-1115 NN denotes placenta
T1001 1116-1117 -LRB- denotes [
T1002 1117-1119 CD denotes 13
T1003 1119-1120 -RRB- denotes ]
T1004 1120-1121 . denotes .
T1005 1121-1225 sentence denotes No expression was found in fetal or adult brain, consistent with a peripheral cause of deafness [2,11].
T1006 1122-1124 DT denotes No
T1007 1125-1135 NN denotes expression
T1009 1136-1139 VBD denotes was
T1008 1140-1145 VBN denotes found
T1010 1146-1148 IN denotes in
T1011 1149-1154 JJ denotes fetal
T1013 1155-1157 CC denotes or
T1014 1158-1163 JJ denotes adult
T1012 1164-1169 NN denotes brain
T1015 1169-1171 , denotes ,
T1016 1171-1181 JJ denotes consistent
T1017 1182-1186 IN denotes with
T1018 1187-1188 DT denotes a
T1020 1189-1199 JJ denotes peripheral
T1019 1200-1205 NN denotes cause
T1021 1206-1208 IN denotes of
T1022 1209-1217 NN denotes deafness
T1023 1218-1219 -LRB- denotes [
T1025 1219-1220 CD denotes 2
T1026 1220-1221 , denotes ,
T1024 1221-1223 CD denotes 11
T1027 1223-1224 -RRB- denotes ]
T1028 1224-1225 . denotes .
T1029 1225-1381 sentence denotes Expression of pendrin mRNA in the inner ear has been found in several places including the cochlea, the vestibular labyrinth and the endolymphatic sac [8].
T1030 1226-1236 NN denotes Expression
T1032 1237-1239 IN denotes of
T1033 1240-1247 NN denotes pendrin
T1034 1248-1252 NN denotes mRNA
T1035 1253-1255 IN denotes in
T1036 1256-1259 DT denotes the
T1038 1260-1265 JJ denotes inner
T1037 1266-1269 NN denotes ear
T1039 1270-1273 VBZ denotes has
T1040 1274-1278 VBN denotes been
T1031 1279-1284 VBN denotes found
T1041 1285-1287 IN denotes in
T1042 1288-1295 JJ denotes several
T1043 1296-1302 NNS denotes places
T1044 1303-1312 VBG denotes including
T1045 1313-1316 DT denotes the
T1046 1317-1324 NN denotes cochlea
T1047 1324-1326 , denotes ,
T1048 1326-1329 DT denotes the
T1050 1330-1340 JJ denotes vestibular
T1049 1341-1350 NN denotes labyrinth
T1051 1351-1354 CC denotes and
T1052 1355-1358 DT denotes the
T1054 1359-1372 JJ denotes endolymphatic
T1053 1373-1376 NN denotes sac
T1055 1377-1378 -LRB- denotes [
T1056 1378-1379 CD denotes 8
T1057 1379-1380 -RRB- denotes ]
T1058 1380-1381 . denotes .
T1059 1381-1471 sentence denotes The precise location of pendrin protein expression, however, has not yet been determined.
T1060 1382-1385 DT denotes The
T1062 1386-1393 JJ denotes precise
T1061 1394-1402 NN denotes location
T1064 1403-1405 IN denotes of
T1065 1406-1413 NN denotes pendrin
T1067 1414-1421 NN denotes protein
T1066 1422-1432 NN denotes expression
T1068 1432-1434 , denotes ,
T1069 1434-1441 RB denotes however
T1070 1441-1443 , denotes ,
T1071 1443-1446 VBZ denotes has
T1072 1447-1450 RB denotes not
T1073 1451-1454 RB denotes yet
T1074 1455-1459 VBN denotes been
T1063 1460-1470 VBN denotes determined
T1075 1470-1471 . denotes .
T1076 1471-1654 sentence denotes The variability of deafness in Pendred syndrome and the observation that deafness is sometimes late in onset suggest that pendrin dysfunction may not be the direct cause of deafness.
T1077 1472-1475 DT denotes The
T1078 1476-1487 NN denotes variability
T1080 1488-1490 IN denotes of
T1081 1491-1499 NN denotes deafness
T1082 1500-1502 IN denotes in
T1083 1503-1510 NNP denotes Pendred
T1084 1511-1519 NN denotes syndrome
T1085 1520-1523 CC denotes and
T1086 1524-1527 DT denotes the
T1087 1528-1539 NN denotes observation
T1088 1540-1544 IN denotes that
T1090 1545-1553 NN denotes deafness
T1089 1554-1556 VBZ denotes is
T1091 1557-1566 RB denotes sometimes
T1092 1567-1571 JJ denotes late
T1093 1572-1574 IN denotes in
T1094 1575-1580 NN denotes onset
T1079 1581-1588 VBP denotes suggest
T1095 1589-1593 IN denotes that
T1097 1594-1601 NN denotes pendrin
T1098 1602-1613 NN denotes dysfunction
T1099 1614-1617 MD denotes may
T1100 1618-1621 RB denotes not
T1096 1622-1624 VB denotes be
T1101 1625-1628 DT denotes the
T1103 1629-1635 JJ denotes direct
T1102 1636-1641 NN denotes cause
T1104 1642-1644 IN denotes of
T1105 1645-1653 NN denotes deafness
T1106 1653-1654 . denotes .
T1107 1654-1812 sentence denotes It is conceivable that pendrin dysfunction favors changes in the expression levels of proteins that are critical for the maintenance of the hearing function.
T1108 1655-1657 PRP denotes It
T1109 1658-1660 VBZ denotes is
T1110 1661-1672 JJ denotes conceivable
T1111 1673-1677 IN denotes that
T1113 1678-1685 NN denotes pendrin
T1114 1686-1697 NN denotes dysfunction
T1112 1698-1704 VBZ denotes favors
T1115 1705-1712 NNS denotes changes
T1116 1713-1715 IN denotes in
T1117 1716-1719 DT denotes the
T1119 1720-1730 NN denotes expression
T1118 1731-1737 NNS denotes levels
T1120 1738-1740 IN denotes of
T1121 1741-1749 NN denotes proteins
T1122 1750-1754 WDT denotes that
T1123 1755-1758 VBP denotes are
T1124 1759-1767 JJ denotes critical
T1125 1768-1771 IN denotes for
T1126 1772-1775 DT denotes the
T1127 1776-1787 NN denotes maintenance
T1128 1788-1790 IN denotes of
T1129 1791-1794 DT denotes the
T1131 1795-1802 NN denotes hearing
T1130 1803-1811 NN denotes function
T1132 1811-1812 . denotes .
T1133 1812-2102 sentence denotes Detailed studies aimed at identifying the direct cause of deafness in Pendred syndrome have recently become possible due to the generation of a pendrin-specific polyclonal antibody [9] and the development of Slc26a4-/- mice, which bear a targeted disruption of the mouse Slc26a4 gene [14].
T1134 1813-1821 JJ denotes Detailed
T1135 1822-1829 NNS denotes studies
T1137 1830-1835 VBN denotes aimed
T1138 1836-1838 IN denotes at
T1139 1839-1850 VBG denotes identifying
T1140 1851-1854 DT denotes the
T1142 1855-1861 JJ denotes direct
T1141 1862-1867 NN denotes cause
T1143 1868-1870 IN denotes of
T1144 1871-1879 NN denotes deafness
T1145 1880-1882 IN denotes in
T1146 1883-1890 NNP denotes Pendred
T1147 1891-1899 NN denotes syndrome
T1148 1900-1904 VBP denotes have
T1149 1905-1913 RB denotes recently
T1136 1914-1920 VB denotes become
T1150 1921-1929 JJ denotes possible
T1151 1930-1933 IN denotes due
T1152 1934-1936 IN denotes to
T1153 1937-1940 DT denotes the
T1154 1941-1951 NN denotes generation
T1155 1952-1954 IN denotes of
T1156 1955-1956 DT denotes a
T1158 1957-1964 NN denotes pendrin
T1160 1964-1965 HYPH denotes -
T1159 1965-1973 JJ denotes specific
T1161 1974-1984 JJ denotes polyclonal
T1157 1985-1993 NN denotes antibody
T1162 1994-1995 -LRB- denotes [
T1163 1995-1996 CD denotes 9
T1164 1996-1997 -RRB- denotes ]
T1165 1998-2001 CC denotes and
T1166 2002-2005 DT denotes the
T1167 2006-2017 NN denotes development
T1168 2018-2020 IN denotes of
T1169 2021-2028 NN denotes Slc26a4
T1171 2028-2029 SYM denotes -
T1172 2029-2030 HYPH denotes /
T1173 2030-2031 SYM denotes -
T1170 2032-2036 NNS denotes mice
T1174 2036-2038 , denotes ,
T1175 2038-2043 WDT denotes which
T1176 2044-2048 VBP denotes bear
T1177 2049-2050 DT denotes a
T1179 2051-2059 JJ denotes targeted
T1178 2060-2070 NN denotes disruption
T1180 2071-2073 IN denotes of
T1181 2074-2077 DT denotes the
T1183 2078-2083 NN denotes mouse
T1184 2084-2091 NN denotes Slc26a4
T1182 2092-2096 NN denotes gene
T1185 2097-2098 -LRB- denotes [
T1186 2098-2100 CD denotes 14
T1187 2100-2101 -RRB- denotes ]
T1188 2101-2102 . denotes .
T1189 2102-2218 sentence denotes The aim in the present study was to determine the location of pendrin and the cause of deafness in Slc26a4-/- mice.
T1190 2103-2106 DT denotes The
T1191 2107-2110 NN denotes aim
T1193 2111-2113 IN denotes in
T1194 2114-2117 DT denotes the
T1196 2118-2125 JJ denotes present
T1195 2126-2131 NN denotes study
T1192 2132-2135 VBD denotes was
T1197 2136-2138 TO denotes to
T1198 2139-2148 VB denotes determine
T1199 2149-2152 DT denotes the
T1200 2153-2161 NN denotes location
T1201 2162-2164 IN denotes of
T1202 2165-2172 NN denotes pendrin
T1203 2173-2176 CC denotes and
T1204 2177-2180 DT denotes the
T1205 2181-2186 NN denotes cause
T1206 2187-2189 IN denotes of
T1207 2190-2198 NN denotes deafness
T1208 2199-2201 IN denotes in
T1209 2202-2209 NN denotes Slc26a4
T1211 2209-2210 SYM denotes -
T1212 2210-2211 HYPH denotes /
T1213 2211-2212 SYM denotes -
T1210 2213-2217 NNS denotes mice
T1214 2217-2218 . denotes .
T1322 2277-2280 CC denotes and
T1323 2281-2294 JJ denotes perilymphatic
T1324 2295-2296 NN denotes K
T1325 2296-2297 SYM denotes +
T1320 2298-2312 NNS denotes concentrations
T1326 2313-2317 VBD denotes were
T1317 2318-2326 VBN denotes measured
T1327 2327-2329 IN denotes in
T1328 2330-2335 JJ denotes young
T1330 2336-2341 JJ denotes adult
T1329 2342-2346 NNS denotes mice
T1331 2347-2348 -LRB- denotes (
T1333 2348-2349 CD denotes 1
T1335 2349-2350 SYM denotes –
T1334 2350-2351 CD denotes 4
T1332 2352-2357 NN denotes month
T1336 2358-2360 IN denotes of
T1337 2361-2364 NN denotes age
T1338 2364-2365 -RRB- denotes )
T1339 2366-2370 WDT denotes that
T1341 2371-2377 CC denotes either
T1340 2378-2382 VBP denotes have
T1342 2383-2384 -LRB- denotes (
T1343 2384-2391 NN denotes Slc26a4
T1344 2391-2392 SYM denotes +
T1345 2392-2393 HYPH denotes /
T1346 2393-2394 SYM denotes +
T1347 2394-2395 -RRB- denotes )
T1348 2396-2398 CC denotes or
T1349 2399-2403 VBP denotes lack
T1350 2404-2405 -LRB- denotes (
T1351 2405-2412 NN denotes Slc26a4
T1352 2412-2413 SYM denotes -
T1353 2413-2414 HYPH denotes /
T1354 2414-2415 SYM denotes -
T1355 2415-2416 -RRB- denotes )
T1356 2417-2418 DT denotes a
T1358 2419-2429 JJ denotes functional
T1357 2430-2434 NN denotes gene
T1359 2435-2438 IN denotes for
T1360 2439-2446 NN denotes pendrin
T1361 2447-2448 -LRB- denotes [
T1362 2448-2450 CD denotes 14
T1363 2450-2451 -RRB- denotes ]
T1364 2451-2452 . denotes .
T1365 2452-2670 sentence denotes The mouse strain 129Sv/Ev (Taconic, Germantown, NY) was chosen as the source of Slc26a4+/+ controls, since Slc26a4-/- mice were propagated in the this strain and generated using a stem cell line derived from 129Sv/Ev.
T1366 2453-2456 DT denotes The
T1368 2457-2462 NN denotes mouse
T1367 2463-2469 NN denotes strain
T1370 2470-2475 NN denotes 129Sv
T1372 2475-2476 HYPH denotes /
T1371 2476-2478 NN denotes Ev
T1373 2479-2480 -LRB- denotes (
T1374 2480-2487 NNP denotes Taconic
T1375 2487-2489 , denotes ,
T1376 2489-2499 NNP denotes Germantown
T1377 2499-2501 , denotes ,
T1378 2501-2503 NNP denotes NY
T1379 2503-2504 -RRB- denotes )
T1380 2505-2508 VBD denotes was
T1369 2509-2515 VBN denotes chosen
T1381 2516-2518 IN denotes as
T1382 2519-2522 DT denotes the
T1383 2523-2529 NN denotes source
T1384 2530-2532 IN denotes of
T1385 2533-2540 NN denotes Slc26a4
T1387 2540-2541 SYM denotes +
T1388 2541-2542 HYPH denotes /
T1389 2542-2543 SYM denotes +
T1386 2544-2552 NNS denotes controls
T1390 2552-2554 , denotes ,
T1391 2554-2559 IN denotes since
T1393 2560-2567 NN denotes Slc26a4
T1395 2567-2568 SYM denotes -
T1396 2568-2569 HYPH denotes /
T1397 2569-2570 SYM denotes -
T1394 2571-2575 NNS denotes mice
T1398 2576-2580 VBD denotes were
T1392 2581-2591 VBN denotes propagated
T1399 2592-2594 IN denotes in
T1400 2595-2598 DT denotes the
T1402 2599-2603 DT denotes this
T1401 2604-2610 NN denotes strain
T1403 2611-2614 CC denotes and
T1404 2615-2624 VBN denotes generated
T1405 2625-2630 VBG denotes using
T1406 2631-2632 DT denotes a
T1408 2633-2637 NN denotes stem
T1409 2638-2642 NN denotes cell
T1407 2643-2647 NN denotes line
T1410 2648-2655 VBN denotes derived
T1411 2656-2660 IN denotes from
T1412 2661-2666 NN denotes 129Sv
T1414 2666-2667 HYPH denotes /
T1413 2667-2669 NN denotes Ev
T1415 2669-2670 . denotes .
T1417 2671-2678 NN denotes Slc26a4
T1416 2671-2709 sentence denotes Slc26a4+/+ and Slc26a4-/- were agouti.
T1419 2678-2679 SYM denotes +
T1420 2679-2680 HYPH denotes /
T1421 2680-2681 SYM denotes +
T1422 2682-2685 CC denotes and
T1423 2686-2693 NN denotes Slc26a4
T1424 2693-2694 SYM denotes -
T1425 2694-2695 HYPH denotes /
T1426 2695-2696 SYM denotes -
T1418 2697-2701 VBD denotes were
T1427 2702-2708 NN denotes agouti
T1428 2708-2709 . denotes .
T1429 2709-2744 sentence denotes They did not differ in coat color.
T1430 2710-2714 PRP denotes They
T1432 2715-2718 VBD denotes did
T1433 2719-2722 RB denotes not
T1431 2723-2729 VB denotes differ
T1434 2730-2732 IN denotes in
T1435 2733-2737 NN denotes coat
T1436 2738-2743 NN denotes color
T1437 2743-2744 . denotes .
T1438 2744-2869 sentence denotes Differences in pigmentation were verified using coisogenic age-matched Slc26a4+/+ and Slc26a4-/- that were bred in parallel.
T1439 2745-2756 NNS denotes Differences
T1441 2757-2759 IN denotes in
T1442 2760-2772 NN denotes pigmentation
T1443 2773-2777 VBD denotes were
T1440 2778-2786 VBN denotes verified
T1444 2787-2792 VBG denotes using
T1445 2793-2803 JJ denotes coisogenic
T1447 2804-2807 NN denotes age
T1449 2807-2808 HYPH denotes -
T1448 2808-2815 VBN denotes matched
T1446 2816-2823 NN denotes Slc26a4
T1450 2823-2824 SYM denotes +
T1451 2824-2825 HYPH denotes /
T1452 2825-2826 SYM denotes +
T1453 2827-2830 CC denotes and
T1454 2831-2838 NN denotes Slc26a4
T1455 2838-2839 SYM denotes -
T1456 2839-2840 HYPH denotes /
T1457 2840-2841 SYM denotes -
T1458 2842-2846 WDT denotes that
T1460 2847-2851 VBD denotes were
T1459 2852-2856 VBN denotes bred
T1461 2857-2859 IN denotes in
T1462 2860-2868 NN denotes parallel
T1463 2868-2869 . denotes .
T1465 2870-2880 NN denotes Expression
T1464 2870-3049 sentence denotes Expression of key proteins involved in the generation of the endocochlear potential and the transport of K+ was studied using confocal immunocytochemistry and quantitative RT-PCR.
T1467 2881-2883 IN denotes of
T1468 2884-2887 JJ denotes key
T1469 2888-2896 NN denotes proteins
T1470 2897-2905 VBN denotes involved
T1471 2906-2908 IN denotes in
T1472 2909-2912 DT denotes the
T1473 2913-2923 NN denotes generation
T1474 2924-2926 IN denotes of
T1475 2927-2930 DT denotes the
T1477 2931-2943 JJ denotes endocochlear
T1476 2944-2953 NN denotes potential
T1478 2954-2957 CC denotes and
T1479 2958-2961 DT denotes the
T1480 2962-2971 NN denotes transport
T1481 2972-2974 IN denotes of
T1482 2975-2976 NN denotes K
T1483 2976-2977 SYM denotes +
T1484 2978-2981 VBD denotes was
T1466 2982-2989 VBN denotes studied
T1485 2990-2995 VBG denotes using
T1486 2996-3004 JJ denotes confocal
T1487 3005-3024 NN denotes immunocytochemistry
T1488 3025-3028 CC denotes and
T1489 3029-3041 JJ denotes quantitative
T1491 3042-3044 NN denotes RT
T1492 3044-3045 HYPH denotes -
T1490 3045-3048 NN denotes PCR
T1493 3048-3049 . denotes .
T1494 3049-3164 sentence denotes K+ secretion and the generation of the endocochlear potential were measured using electrophysiological techniques.
T1495 3050-3051 NN denotes K
T1497 3051-3052 SYM denotes +
T1496 3053-3062 NN denotes secretion
T1499 3063-3066 CC denotes and
T1500 3067-3070 DT denotes the
T1501 3071-3081 NN denotes generation
T1502 3082-3084 IN denotes of
T1503 3085-3088 DT denotes the
T1505 3089-3101 JJ denotes endocochlear
T1504 3102-3111 NN denotes potential
T1506 3112-3116 VBD denotes were
T1498 3117-3125 VBN denotes measured
T1507 3126-3131 VBG denotes using
T1508 3132-3152 JJ denotes electrophysiological
T1509 3153-3163 NNS denotes techniques
T1510 3163-3164 . denotes .
T1511 3164-3273 sentence denotes All experiments were approved by the Institutional Animal Care and Use Committee of Kansas State University.
T1512 3165-3168 DT denotes All
T1513 3169-3180 NNS denotes experiments
T1515 3181-3185 VBD denotes were
T1514 3186-3194 VBN denotes approved
T1516 3195-3197 IN denotes by
T1517 3198-3201 DT denotes the
T1519 3202-3215 NNP denotes Institutional
T1520 3216-3222 NNP denotes Animal
T1521 3223-3227 NNP denotes Care
T1522 3228-3231 CC denotes and
T1523 3232-3235 NNP denotes Use
T1518 3236-3245 NNP denotes Committee
T1524 3246-3248 IN denotes of
T1525 3249-3255 NNP denotes Kansas
T1527 3256-3261 NNP denotes State
T1526 3262-3272 NNP denotes University
T1528 3272-3273 . denotes .
T1678 3275-3283 JJ denotes Confocal
T1679 3284-3303 NN denotes immunocytochemistry
T1680 3303-3496 sentence denotes Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.) and transcardially perfused with a Cl--free phosphate-buffered Na-gluconate solution containing 4% paraformaldehyde.
T1681 3304-3311 NNS denotes Animals
T1683 3312-3316 VBD denotes were
T1684 3317-3323 RB denotes deeply
T1682 3324-3336 VBN denotes anesthetized
T1685 3337-3341 IN denotes with
T1686 3342-3348 NN denotes sodium
T1687 3349-3362 NN denotes pentobarbital
T1688 3363-3364 -LRB- denotes (
T1690 3364-3367 CD denotes 100
T1689 3368-3370 NNS denotes mg
T1691 3370-3371 SYM denotes /
T1692 3371-3373 NN denotes kg
T1693 3374-3378 RB denotes i.p.
T1694 3378-3379 -RRB- denotes )
T1695 3380-3383 CC denotes and
T1696 3384-3398 RB denotes transcardially
T1697 3399-3407 VBN denotes perfused
T1698 3408-3412 IN denotes with
T1699 3413-3414 DT denotes a
T1701 3415-3417 NN denotes Cl
T1703 3417-3418 SYM denotes -
T1704 3418-3419 HYPH denotes -
T1702 3419-3423 JJ denotes free
T1705 3424-3433 NN denotes phosphate
T1707 3433-3434 HYPH denotes -
T1706 3434-3442 VBN denotes buffered
T1708 3443-3445 NN denotes Na
T1710 3445-3446 SYM denotes -
T1709 3446-3455 NN denotes gluconate
T1700 3456-3464 NN denotes solution
T1711 3465-3475 VBG denotes containing
T1712 3476-3477 CD denotes 4
T1713 3477-3478 NN denotes %
T1714 3479-3495 NN denotes paraformaldehyde
T1715 3495-3496 . denotes .
T1716 3496-3671 sentence denotes Temporal bones were removed and cochleae fixed by perilymphatic perfusion, decalcified in EDTA, processed through a sucrose gradient and infiltrated with polyethylene glycol.
T1717 3497-3505 JJ denotes Temporal
T1718 3506-3511 NNS denotes bones
T1720 3512-3516 VBD denotes were
T1719 3517-3524 VBN denotes removed
T1721 3525-3528 CC denotes and
T1722 3529-3537 NNS denotes cochleae
T1723 3538-3543 VBN denotes fixed
T1724 3544-3546 IN denotes by
T1725 3547-3560 JJ denotes perilymphatic
T1726 3561-3570 NN denotes perfusion
T1727 3570-3572 , denotes ,
T1728 3572-3583 VBN denotes decalcified
T1729 3584-3586 IN denotes in
T1730 3587-3591 NN denotes EDTA
T1731 3591-3593 , denotes ,
T1732 3593-3602 VBN denotes processed
T1733 3603-3610 IN denotes through
T1734 3611-3612 DT denotes a
T1736 3613-3620 NN denotes sucrose
T1735 3621-3629 NN denotes gradient
T1737 3630-3633 CC denotes and
T1738 3634-3645 VBN denotes infiltrated
T1739 3646-3650 IN denotes with
T1740 3651-3663 NN denotes polyethylene
T1741 3664-3670 NN denotes glycol
T1742 3670-3671 . denotes .
T1743 3671-3818 sentence denotes Mid-modiolar cryosections (12 μm, CM3050S, Leica, Nussloch, Germany) were blocked in PBS with 0.2% Triton-X (PBS-TX) and 10% bovine serum albumin.
T1744 3672-3684 JJ denotes Mid-modiolar
T1745 3685-3697 NNS denotes cryosections
T1747 3698-3699 -LRB- denotes (
T1749 3699-3701 CD denotes 12
T1750 3702-3704 NNS denotes μm
T1751 3704-3706 , denotes ,
T1748 3706-3713 NN denotes CM3050S
T1752 3713-3715 , denotes ,
T1753 3715-3720 NNP denotes Leica
T1754 3720-3722 , denotes ,
T1755 3722-3730 NNP denotes Nussloch
T1756 3730-3732 , denotes ,
T1757 3732-3739 NNP denotes Germany
T1758 3739-3740 -RRB- denotes )
T1759 3741-3745 VBD denotes were
T1746 3746-3753 VBN denotes blocked
T1760 3754-3756 IN denotes in
T1761 3757-3760 NN denotes PBS
T1762 3761-3765 IN denotes with
T1763 3766-3769 CD denotes 0.2
T1764 3769-3770 NN denotes %
T1766 3771-3777 NN denotes Triton
T1767 3777-3778 HYPH denotes -
T1765 3778-3779 NN denotes X
T1768 3780-3781 -LRB- denotes (
T1770 3781-3784 NN denotes PBS
T1771 3784-3785 HYPH denotes -
T1769 3785-3787 NN denotes TX
T1772 3787-3788 -RRB- denotes )
T1773 3789-3792 CC denotes and
T1774 3793-3795 CD denotes 10
T1775 3795-3796 NN denotes %
T1777 3797-3803 JJ denotes bovine
T1778 3804-3809 NN denotes serum
T1776 3810-3817 NN denotes albumin
T1779 3817-3818 . denotes .
T1780 3818-4259 sentence denotes Slides were incubated overnight at 4°C with primary antibodies in PBS-TX with 1–3% BSA [rabbit anti-pendrin, 1:500 (h766–780); rat anti-ZO-1, 1:100 (Chemicon, Temecula, CA); goat anti-KCNQ1, 1:200 (C20, Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-KCNE1, 1:200 (Alomone, Jerusalem, Israel); rabbit anti-KCNJ10, 1:300 (Alomone); rabbit anti-SLC12A2, 1:100 (Chemicon); and rabbit anti-connexin 26, 1:100 (Zymed, San Francisco, CA)].
T1781 3819-3825 NNS denotes Slides
T1783 3826-3830 VBD denotes were
T1782 3831-3840 VBN denotes incubated
T1784 3841-3850 RB denotes overnight
T1785 3851-3853 IN denotes at
T1786 3854-3855 CD denotes 4
T1787 3855-3857 NN denotes °C
T1788 3858-3862 IN denotes with
T1789 3863-3870 JJ denotes primary
T1790 3871-3881 NNS denotes antibodies
T1791 3882-3884 IN denotes in
T1792 3885-3888 NN denotes PBS
T1794 3888-3889 HYPH denotes -
T1793 3889-3891 NN denotes TX
T1795 3892-3896 IN denotes with
T1796 3897-3898 CD denotes 1
T1798 3898-3899 SYM denotes –
T1797 3899-3900 CD denotes 3
T1799 3900-3901 NN denotes %
T1800 3902-3905 NN denotes BSA
T1801 3906-3907 -LRB- denotes [
T1803 3907-3913 NN denotes rabbit
T1804 3914-3926 NN denotes anti-pendrin
T1805 3926-3928 , denotes ,
T1806 3928-3929 CD denotes 1
T1807 3929-3930 SYM denotes :
T1808 3930-3933 CD denotes 500
T1809 3934-3935 -LRB- denotes (
T1802 3935-3939 NN denotes h766
T1810 3939-3940 SYM denotes –
T1811 3940-3943 CD denotes 780
T1812 3943-3944 -RRB- denotes )
T1813 3944-3945 , denotes ;
T1814 3946-3949 NN denotes rat
T1815 3950-3957 NN denotes anti-ZO
T1817 3957-3958 HYPH denotes -
T1818 3958-3959 CD denotes 1
T1819 3959-3961 , denotes ,
T1820 3961-3962 CD denotes 1
T1821 3962-3963 SYM denotes :
T1822 3963-3966 CD denotes 100
T1823 3967-3968 -LRB- denotes (
T1816 3968-3976 NNP denotes Chemicon
T1824 3976-3978 , denotes ,
T1825 3978-3986 NNP denotes Temecula
T1826 3986-3988 , denotes ,
T1827 3988-3990 NNP denotes CA
T1828 3990-3991 -RRB- denotes )
T1829 3991-3992 , denotes ;
T1830 3993-3997 NN denotes goat
T1831 3998-4008 NN denotes anti-KCNQ1
T1833 4008-4010 , denotes ,
T1834 4010-4011 CD denotes 1
T1835 4011-4012 SYM denotes :
T1836 4012-4015 CD denotes 200
T1837 4016-4017 -LRB- denotes (
T1832 4017-4020 NN denotes C20
T1838 4020-4022 , denotes ,
T1839 4022-4027 NNP denotes Santa
T1840 4028-4032 NNP denotes Cruz
T1841 4033-4046 NNP denotes Biotechnology
T1842 4046-4048 , denotes ,
T1843 4048-4053 NNP denotes Santa
T1844 4054-4058 NNP denotes Cruz
T1845 4058-4060 , denotes ,
T1846 4060-4062 NNP denotes CA
T1847 4062-4063 -RRB- denotes )
T1848 4063-4065 , denotes ,
T1849 4065-4071 NN denotes rabbit
T1850 4072-4082 NN denotes anti-KCNE1
T1852 4082-4084 , denotes ,
T1853 4084-4085 CD denotes 1
T1854 4085-4086 SYM denotes :
T1855 4086-4089 CD denotes 200
T1856 4090-4091 -LRB- denotes (
T1851 4091-4098 NNP denotes Alomone
T1857 4098-4100 , denotes ,
T1858 4100-4109 NNP denotes Jerusalem
T1859 4109-4111 , denotes ,
T1860 4111-4117 NNP denotes Israel
T1861 4117-4118 -RRB- denotes )
T1862 4118-4119 , denotes ;
T1863 4120-4126 NN denotes rabbit
T1864 4127-4138 NN denotes anti-KCNJ10
T1866 4138-4140 , denotes ,
T1867 4140-4141 CD denotes 1
T1868 4141-4142 SYM denotes :
T1869 4142-4145 CD denotes 300
T1870 4146-4147 -LRB- denotes (
T1865 4147-4154 NNP denotes Alomone
T1871 4154-4155 -RRB- denotes )
T1872 4155-4156 , denotes ;
T1873 4157-4163 NN denotes rabbit
T1874 4164-4176 NN denotes anti-SLC12A2
T1876 4176-4178 , denotes ,
T1877 4178-4179 CD denotes 1
T1878 4179-4180 SYM denotes :
T1879 4180-4183 CD denotes 100
T1880 4184-4185 -LRB- denotes (
T1875 4185-4193 NNP denotes Chemicon
T1881 4193-4194 -RRB- denotes )
T1882 4194-4195 : denotes ;
T1883 4196-4199 CC denotes and
T1884 4200-4206 NN denotes rabbit
T1885 4207-4220 NN denotes anti-connexin
T1887 4221-4223 CD denotes 26
T1888 4223-4225 , denotes ,
T1889 4225-4226 CD denotes 1
T1890 4226-4227 SYM denotes :
T1891 4227-4230 CD denotes 100
T1892 4231-4232 -LRB- denotes (
T1886 4232-4237 NNP denotes Zymed
T1893 4237-4239 , denotes ,
T1894 4239-4242 NNP denotes San
T1895 4243-4252 NNP denotes Francisco
T1896 4252-4254 , denotes ,
T1897 4254-4256 NNP denotes CA
T1898 4256-4257 -RRB- denotes )
T1899 4257-4258 -RRB- denotes ]
T1900 4258-4259 . denotes .
T1901 4259-4525 sentence denotes Slides were washed in PBS-TX and incubated for 1 h at 25°C with appropriate secondary antibodies at a 1:1000 dilution in PBS-TX with 1–3% BSA [donkey anti-rabbit Alexa 488, chicken anti-rat Alexa 594, and chicken anti-goat Alexa 594 (Molecular Probes, Eugene, OR)].
T1902 4260-4266 NNS denotes Slides
T1904 4267-4271 VBD denotes were
T1903 4272-4278 VBN denotes washed
T1905 4279-4281 IN denotes in
T1906 4282-4285 NN denotes PBS
T1908 4285-4286 HYPH denotes -
T1907 4286-4288 NN denotes TX
T1909 4289-4292 CC denotes and
T1910 4293-4302 VBN denotes incubated
T1911 4303-4306 IN denotes for
T1912 4307-4308 CD denotes 1
T1913 4309-4310 NN denotes h
T1914 4311-4313 IN denotes at
T1915 4314-4316 CD denotes 25
T1916 4316-4318 NN denotes °C
T1917 4319-4323 IN denotes with
T1918 4324-4335 JJ denotes appropriate
T1920 4336-4345 JJ denotes secondary
T1919 4346-4356 NNS denotes antibodies
T1921 4357-4359 IN denotes at
T1922 4360-4361 DT denotes a
T1924 4362-4363 CD denotes 1
T1925 4363-4364 SYM denotes :
T1926 4364-4368 CD denotes 1000
T1923 4369-4377 NN denotes dilution
T1927 4378-4380 IN denotes in
T1928 4381-4384 NN denotes PBS
T1930 4384-4385 HYPH denotes -
T1929 4385-4387 NN denotes TX
T1931 4388-4392 IN denotes with
T1932 4393-4394 CD denotes 1
T1934 4394-4395 SYM denotes –
T1933 4395-4396 CD denotes 3
T1935 4396-4397 NN denotes %
T1936 4398-4401 NN denotes BSA
T1937 4402-4403 -LRB- denotes [
T1939 4403-4409 NN denotes donkey
T1941 4410-4421 JJ denotes anti-rabbit
T1940 4422-4427 NNP denotes Alexa
T1942 4428-4431 CD denotes 488
T1943 4431-4433 , denotes ,
T1944 4433-4440 NN denotes chicken
T1946 4441-4449 JJ denotes anti-rat
T1945 4450-4455 NNP denotes Alexa
T1947 4456-4459 CD denotes 594
T1948 4459-4461 , denotes ,
T1949 4461-4464 CC denotes and
T1950 4465-4472 NN denotes chicken
T1952 4473-4482 JJ denotes anti-goat
T1951 4483-4488 NNP denotes Alexa
T1953 4489-4492 CD denotes 594
T1954 4493-4494 -LRB- denotes (
T1955 4494-4503 NNP denotes Molecular
T1938 4504-4510 NNP denotes Probes
T1956 4510-4512 , denotes ,
T1957 4512-4518 NNP denotes Eugene
T1958 4518-4520 , denotes ,
T1959 4520-4522 NNP denotes OR
T1960 4522-4523 -RRB- denotes )
T1961 4523-4524 -RRB- denotes ]
T1962 4524-4525 . denotes .
T1963 4525-4626 sentence denotes Actin filaments were visualized by staining with Alexa 488 conjugated phalloidin (Molecular Probes).
T1964 4526-4531 NN denotes Actin
T1965 4532-4541 NNS denotes filaments
T1967 4542-4546 VBD denotes were
T1966 4547-4557 VBN denotes visualized
T1968 4558-4560 IN denotes by
T1969 4561-4569 VBG denotes staining
T1970 4570-4574 IN denotes with
T1971 4575-4580 NNP denotes Alexa
T1973 4581-4584 CD denotes 488
T1972 4585-4595 VBN denotes conjugated
T1974 4596-4606 NN denotes phalloidin
T1975 4607-4608 -LRB- denotes (
T1977 4608-4617 NNP denotes Molecular
T1976 4618-4624 NNP denotes Probes
T1978 4624-4625 -RRB- denotes )
T1979 4625-4626 . denotes .
T1980 4626-4833 sentence denotes After incubation, slides were washed with PBS-TX, mounted with FluorSave (Calbiochem, La Jolla, CA), and viewed by confocal microscopy (LSM 5 Pascal or LSM 510 Meta, Carl Zeiss, Jena or Göttingen, Germany).
T1981 4627-4632 IN denotes After
T1983 4633-4643 NN denotes incubation
T1984 4643-4645 , denotes ,
T1985 4645-4651 NNS denotes slides
T1986 4652-4656 VBD denotes were
T1982 4657-4663 VBN denotes washed
T1987 4664-4668 IN denotes with
T1988 4669-4672 NN denotes PBS
T1990 4672-4673 HYPH denotes -
T1989 4673-4675 NN denotes TX
T1991 4675-4677 , denotes ,
T1992 4677-4684 VBN denotes mounted
T1993 4685-4689 IN denotes with
T1994 4690-4699 NNP denotes FluorSave
T1995 4700-4701 -LRB- denotes (
T1996 4701-4711 NNP denotes Calbiochem
T1997 4711-4713 , denotes ,
T1998 4713-4715 NNP denotes La
T1999 4716-4721 NNP denotes Jolla
T2000 4721-4723 , denotes ,
T2001 4723-4725 NNP denotes CA
T2002 4725-4726 -RRB- denotes )
T2003 4726-4728 , denotes ,
T2004 4728-4731 CC denotes and
T2005 4732-4738 VBN denotes viewed
T2006 4739-4741 IN denotes by
T2007 4742-4750 JJ denotes confocal
T2008 4751-4761 NN denotes microscopy
T2009 4762-4763 -LRB- denotes (
T2011 4763-4766 NN denotes LSM
T2012 4767-4768 CD denotes 5
T2010 4769-4775 NN denotes Pascal
T2013 4776-4778 CC denotes or
T2014 4779-4782 NN denotes LSM
T2016 4783-4786 CD denotes 510
T2015 4787-4791 NN denotes Meta
T2017 4791-4793 , denotes ,
T2018 4793-4797 NNP denotes Carl
T2019 4798-4803 NNP denotes Zeiss
T2020 4803-4805 , denotes ,
T2021 4805-4809 NNP denotes Jena
T2022 4810-4812 CC denotes or
T2023 4813-4822 NNP denotes Göttingen
T2024 4822-4824 , denotes ,
T2025 4824-4831 NNP denotes Germany
T2026 4831-4832 -RRB- denotes )
T2027 4832-4833 . denotes .
T2028 4833-4979 sentence denotes Laser scanning brightfield images were collected to document structural preservation, for morphometric analysis and for analysis of pigmentation.
T2029 4834-4839 NN denotes Laser
T2030 4840-4848 NN denotes scanning
T2032 4849-4860 NN denotes brightfield
T2031 4861-4867 NNS denotes images
T2034 4868-4872 VBD denotes were
T2033 4873-4882 VBN denotes collected
T2035 4883-4885 TO denotes to
T2036 4886-4894 VB denotes document
T2037 4895-4905 JJ denotes structural
T2038 4906-4918 NN denotes preservation
T2039 4918-4920 , denotes ,
T2040 4920-4923 IN denotes for
T2041 4924-4936 JJ denotes morphometric
T2042 4937-4945 NN denotes analysis
T2043 4946-4949 CC denotes and
T2044 4950-4953 IN denotes for
T2045 4954-4962 NN denotes analysis
T2046 4963-4965 IN denotes of
T2047 4966-4978 NN denotes pigmentation
T2048 4978-4979 . denotes .
T2210 4981-4993 JJ denotes Quantitative
T2212 4994-4996 NN denotes RT
T2213 4996-4997 HYPH denotes -
T2211 4997-5000 NN denotes PCR
T2214 5000-5077 sentence denotes Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.).
T2215 5001-5008 NNS denotes Animals
T2217 5009-5013 VBD denotes were
T2218 5014-5020 RB denotes deeply
T2216 5021-5033 VBN denotes anesthetized
T2219 5034-5038 IN denotes with
T2220 5039-5045 NN denotes sodium
T2221 5046-5059 NN denotes pentobarbital
T2222 5060-5061 -LRB- denotes (
T2224 5061-5064 CD denotes 100
T2223 5065-5067 NN denotes mg
T2225 5067-5068 SYM denotes /
T2226 5068-5070 NN denotes kg
T2227 5071-5075 RB denotes i.p.
T2228 5075-5076 -RRB- denotes )
T2229 5076-5077 . denotes .
T2230 5077-5137 sentence denotes Kidneys and brain were removed and pulverized in liquid N2.
T2231 5078-5085 NNS denotes Kidneys
T2233 5086-5089 CC denotes and
T2234 5090-5095 NN denotes brain
T2235 5096-5100 VBD denotes were
T2232 5101-5108 VBN denotes removed
T2236 5109-5112 CC denotes and
T2237 5113-5123 VBN denotes pulverized
T2238 5124-5126 IN denotes in
T2239 5127-5133 NN denotes liquid
T2240 5134-5136 NN denotes N2
T2241 5136-5137 . denotes .
T2242 5137-5365 sentence denotes Temporal bones were removed and two preparations of inner ear tissues were obtained by microdissection: (1) stria vascularis without spiral ligament, and (2) spiral ganglia including the surrounding bone and the organ of Corti.
T2243 5138-5146 JJ denotes Temporal
T2244 5147-5152 NNS denotes bones
T2246 5153-5157 VBD denotes were
T2245 5158-5165 VBN denotes removed
T2247 5166-5169 CC denotes and
T2248 5170-5173 CD denotes two
T2249 5174-5186 NNS denotes preparations
T2251 5187-5189 IN denotes of
T2252 5190-5195 JJ denotes inner
T2254 5196-5199 NN denotes ear
T2253 5200-5207 NNS denotes tissues
T2255 5208-5212 VBD denotes were
T2250 5213-5221 VBN denotes obtained
T2256 5222-5224 IN denotes by
T2257 5225-5240 NN denotes microdissection
T2258 5240-5242 : denotes :
T2259 5242-5243 -LRB- denotes (
T2260 5243-5244 LS denotes 1
T2262 5244-5245 -RRB- denotes )
T2263 5246-5251 NN denotes stria
T2261 5252-5262 NN denotes vascularis
T2264 5263-5270 IN denotes without
T2265 5271-5277 JJ denotes spiral
T2266 5278-5286 NN denotes ligament
T2267 5286-5288 , denotes ,
T2268 5288-5291 CC denotes and
T2269 5292-5293 -LRB- denotes (
T2270 5293-5294 LS denotes 2
T2272 5294-5295 -RRB- denotes )
T2273 5296-5302 JJ denotes spiral
T2271 5303-5310 NNS denotes ganglia
T2274 5311-5320 VBG denotes including
T2275 5321-5324 DT denotes the
T2277 5325-5336 VBG denotes surrounding
T2276 5337-5341 NN denotes bone
T2278 5342-5345 CC denotes and
T2279 5346-5349 DT denotes the
T2280 5350-5355 NN denotes organ
T2281 5356-5358 IN denotes of
T2282 5359-5364 NNP denotes Corti
T2283 5364-5365 . denotes .
T2284 5365-5510 sentence denotes The dissection medium was changed twice during the microdissection and isolated tissues were washed three times to minimize cross-contamination.
T2285 5366-5369 DT denotes The
T2287 5370-5380 NN denotes dissection
T2286 5381-5387 NN denotes medium
T2289 5388-5391 VBD denotes was
T2288 5392-5399 VBN denotes changed
T2290 5400-5405 RB denotes twice
T2291 5406-5412 IN denotes during
T2292 5413-5416 DT denotes the
T2293 5417-5432 NN denotes microdissection
T2294 5433-5436 CC denotes and
T2295 5437-5445 VBN denotes isolated
T2296 5446-5453 NNS denotes tissues
T2298 5454-5458 VBD denotes were
T2297 5459-5465 VBN denotes washed
T2299 5466-5471 CD denotes three
T2300 5472-5477 NNS denotes times
T2301 5478-5480 TO denotes to
T2302 5481-5489 VB denotes minimize
T2303 5490-5509 NN denotes cross-contamination
T2304 5509-5510 . denotes .
T2305 5510-5613 sentence denotes The Slc26a4-/-genotype was verified by the observation of large otoconia in the utricular macula [14].
T2306 5511-5514 DT denotes The
T2308 5515-5522 NN denotes Slc26a4
T2309 5522-5523 SYM denotes -
T2310 5523-5524 HYPH denotes /
T2311 5524-5525 SYM denotes -
T2307 5525-5533 NN denotes genotype
T2313 5534-5537 VBD denotes was
T2312 5538-5546 VBN denotes verified
T2314 5547-5549 IN denotes by
T2315 5550-5553 DT denotes the
T2316 5554-5565 NN denotes observation
T2317 5566-5568 IN denotes of
T2318 5569-5574 JJ denotes large
T2319 5575-5583 NN denotes otoconia
T2320 5584-5586 IN denotes in
T2321 5587-5590 DT denotes the
T2323 5591-5600 JJ denotes utricular
T2322 5601-5607 NN denotes macula
T2324 5608-5609 -LRB- denotes [
T2325 5609-5611 CD denotes 14
T2326 5611-5612 -RRB- denotes ]
T2327 5612-5613 . denotes .
T2328 5613-5740 sentence denotes Total RNA was isolated and residual DNA contamination was removed by enzymatic digestion (RNeasy micro, Qiagen, Valencia, CA).
T2329 5614-5619 JJ denotes Total
T2330 5620-5623 NN denotes RNA
T2332 5624-5627 VBD denotes was
T2331 5628-5636 VBN denotes isolated
T2333 5637-5640 CC denotes and
T2334 5641-5649 JJ denotes residual
T2336 5650-5653 NN denotes DNA
T2335 5654-5667 NN denotes contamination
T2338 5668-5671 VBD denotes was
T2337 5672-5679 VBN denotes removed
T2339 5680-5682 IN denotes by
T2340 5683-5692 JJ denotes enzymatic
T2341 5693-5702 NN denotes digestion
T2342 5703-5704 -LRB- denotes (
T2344 5704-5710 NN denotes RNeasy
T2343 5711-5716 NN denotes micro
T2345 5716-5718 , denotes ,
T2346 5718-5724 NNP denotes Qiagen
T2347 5724-5726 , denotes ,
T2348 5726-5734 NNP denotes Valencia
T2349 5734-5736 , denotes ,
T2350 5736-5738 NNP denotes CA
T2351 5738-5739 -RRB- denotes )
T2352 5739-5740 . denotes .
T2353 5740-5877 sentence denotes Quality and quantity of 18S rRNA obtained from kidneys and brain were determined (Nano Assay, 2100 Bioanalyzer, Agilent, Palo Alto, CA).
T2354 5741-5748 NN denotes Quality
T2356 5749-5752 CC denotes and
T2357 5753-5761 NN denotes quantity
T2358 5762-5764 IN denotes of
T2359 5765-5768 NN denotes 18S
T2360 5769-5773 NN denotes rRNA
T2361 5774-5782 VBN denotes obtained
T2362 5783-5787 IN denotes from
T2363 5788-5795 NNS denotes kidneys
T2364 5796-5799 CC denotes and
T2365 5800-5805 NN denotes brain
T2366 5806-5810 VBD denotes were
T2355 5811-5821 VBN denotes determined
T2367 5822-5823 -LRB- denotes (
T2369 5823-5827 NNP denotes Nano
T2368 5828-5833 NNP denotes Assay
T2370 5833-5835 , denotes ,
T2371 5835-5839 CD denotes 2100
T2372 5840-5851 NNP denotes Bioanalyzer
T2373 5851-5853 , denotes ,
T2374 5853-5860 NNP denotes Agilent
T2375 5860-5862 , denotes ,
T2376 5862-5866 NNP denotes Palo
T2377 5867-5871 NNP denotes Alto
T2378 5871-5873 , denotes ,
T2379 5873-5875 NNP denotes CA
T2380 5875-5876 -RRB- denotes )
T2381 5876-5877 . denotes .
T2382 5877-6013 sentence denotes Further, the quality of RNA preparations obtained from stria vascularis and spiral ganglia was assessed (Pico Assay, 2100 Bioanalyzer).
T2383 5878-5885 RB denotes Further
T2385 5885-5887 , denotes ,
T2386 5887-5890 DT denotes the
T2387 5891-5898 NN denotes quality
T2388 5899-5901 IN denotes of
T2389 5902-5905 NN denotes RNA
T2390 5906-5918 NNS denotes preparations
T2391 5919-5927 VBN denotes obtained
T2392 5928-5932 IN denotes from
T2393 5933-5938 NN denotes stria
T2394 5939-5949 NN denotes vascularis
T2395 5950-5953 CC denotes and
T2396 5954-5960 JJ denotes spiral
T2397 5961-5968 NNS denotes ganglia
T2398 5969-5972 VBD denotes was
T2384 5973-5981 VBN denotes assessed
T2399 5982-5983 -LRB- denotes (
T2401 5983-5987 NNP denotes Pico
T2400 5988-5993 NNP denotes Assay
T2402 5993-5995 , denotes ,
T2403 5995-5999 CD denotes 2100
T2404 6000-6011 NNP denotes Bioanalyzer
T2405 6011-6012 -RRB- denotes )
T2406 6012-6013 . denotes .
T2407 6013-6204 sentence denotes Real time RT-PCR was performed on RNA obtained from individual animals (OneStep RT-PCR, Qiagen; Smart Cycler, Cepheid, Sunnyvale, CA) in the presence of 0.2× SYBR green I (Molecular Probes).
T2408 6014-6018 JJ denotes Real
T2409 6019-6023 NN denotes time
T2411 6024-6026 NN denotes RT
T2412 6026-6027 HYPH denotes -
T2410 6027-6030 NN denotes PCR
T2414 6031-6034 VBD denotes was
T2413 6035-6044 VBN denotes performed
T2415 6045-6047 IN denotes on
T2416 6048-6051 NN denotes RNA
T2417 6052-6060 VBN denotes obtained
T2418 6061-6065 IN denotes from
T2419 6066-6076 JJ denotes individual
T2420 6077-6084 NNS denotes animals
T2421 6085-6086 -LRB- denotes (
T2423 6086-6093 NNP denotes OneStep
T2425 6094-6096 NN denotes RT
T2426 6096-6097 HYPH denotes -
T2424 6097-6100 NN denotes PCR
T2427 6100-6102 , denotes ,
T2428 6102-6108 NNP denotes Qiagen
T2429 6108-6109 : denotes ;
T2430 6110-6115 NNP denotes Smart
T2422 6116-6122 NNP denotes Cycler
T2431 6122-6124 , denotes ,
T2432 6124-6131 NNP denotes Cepheid
T2433 6131-6133 , denotes ,
T2434 6133-6142 NNP denotes Sunnyvale
T2435 6142-6144 , denotes ,
T2436 6144-6146 NNP denotes CA
T2437 6146-6147 -RRB- denotes )
T2438 6148-6150 IN denotes in
T2439 6151-6154 DT denotes the
T2440 6155-6163 NN denotes presence
T2441 6164-6166 IN denotes of
T2442 6167-6170 CD denotes 0.2
T2444 6170-6171 SYM denotes ×
T2443 6172-6176 NN denotes SYBR
T2446 6177-6182 JJ denotes green
T2445 6183-6184 NN denotes I
T2447 6185-6186 -LRB- denotes (
T2449 6186-6195 NNP denotes Molecular
T2448 6196-6202 NNP denotes Probes
T2450 6202-6203 -RRB- denotes )
T2451 6203-6204 . denotes .
T2452 6204-6335 sentence denotes Transcripts of 18S rRNA and mRNA for the K+ channels KCNJ10, KCNQ1 and KCNQ4 were amplified using gene-specific primers (Table 1).
T2453 6205-6216 NNS denotes Transcripts
T2455 6217-6219 IN denotes of
T2456 6220-6223 NN denotes 18S
T2457 6224-6228 NN denotes rRNA
T2458 6229-6232 CC denotes and
T2459 6233-6237 NN denotes mRNA
T2460 6238-6241 IN denotes for
T2461 6242-6245 DT denotes the
T2463 6246-6247 NN denotes K
T2464 6247-6248 SYM denotes +
T2462 6249-6257 NNS denotes channels
T2465 6258-6264 NN denotes KCNJ10
T2466 6264-6266 , denotes ,
T2467 6266-6271 NN denotes KCNQ1
T2468 6272-6275 CC denotes and
T2469 6276-6281 NN denotes KCNQ4
T2470 6282-6286 VBD denotes were
T2454 6287-6296 VBN denotes amplified
T2471 6297-6302 VBG denotes using
T2472 6303-6307 NN denotes gene
T2474 6307-6308 HYPH denotes -
T2473 6308-6316 JJ denotes specific
T2475 6317-6324 NNS denotes primers
T2476 6325-6326 -LRB- denotes (
T2477 6326-6331 NN denotes Table
T2478 6332-6333 CD denotes 1
T2479 6333-6334 -RRB- denotes )
T2480 6334-6335 . denotes .
T2481 6335-6391 sentence denotes RT was performed for 30 min at 50°C and 15 min at 95°C.
T2482 6336-6338 NN denotes RT
T2484 6339-6342 VBD denotes was
T2483 6343-6352 VBN denotes performed
T2485 6353-6356 IN denotes for
T2486 6357-6359 CD denotes 30
T2487 6360-6363 NN denotes min
T2488 6364-6366 IN denotes at
T2489 6367-6369 CD denotes 50
T2490 6369-6371 NN denotes °C
T2491 6372-6375 CC denotes and
T2492 6376-6378 CD denotes 15
T2493 6379-6382 NN denotes min
T2494 6383-6385 IN denotes at
T2495 6386-6388 CD denotes 95
T2496 6388-6390 NN denotes °C
T2497 6390-6391 . denotes .
T2498 6391-6586 sentence denotes PCR consisted of 50 cycles of 1 min at 60°C, 1 min at 72°C, 7s heating to hot-measurement temperature, 13s hot-measurement at 3–5°C below product melting temperature (Table 1) and 1 min at 94°C.
T2499 6392-6395 NN denotes PCR
T2500 6396-6405 VBD denotes consisted
T2501 6406-6408 IN denotes of
T2502 6409-6411 CD denotes 50
T2503 6412-6418 NNS denotes cycles
T2504 6419-6421 IN denotes of
T2505 6422-6423 CD denotes 1
T2506 6424-6427 NN denotes min
T2507 6428-6430 IN denotes at
T2508 6431-6433 CD denotes 60
T2509 6433-6435 NN denotes °C
T2510 6435-6437 , denotes ,
T2511 6437-6438 CD denotes 1
T2512 6439-6442 NN denotes min
T2513 6443-6445 IN denotes at
T2514 6446-6448 CD denotes 72
T2515 6448-6450 NN denotes °C
T2516 6450-6452 , denotes ,
T2517 6452-6454 NN denotes 7s
T2518 6455-6462 VBG denotes heating
T2519 6463-6465 IN denotes to
T2520 6466-6469 JJ denotes hot
T2522 6469-6470 HYPH denotes -
T2521 6470-6481 NN denotes measurement
T2523 6482-6493 NN denotes temperature
T2524 6493-6495 , denotes ,
T2525 6495-6498 NNS denotes 13s
T2527 6499-6502 NN denotes hot
T2528 6502-6503 HYPH denotes -
T2526 6503-6514 NN denotes measurement
T2529 6515-6517 IN denotes at
T2530 6518-6519 CD denotes 3
T2532 6519-6520 SYM denotes –
T2531 6520-6521 CD denotes 5
T2533 6521-6523 NN denotes °C
T2534 6524-6529 IN denotes below
T2535 6530-6537 NN denotes product
T2537 6538-6545 NN denotes melting
T2536 6546-6557 NN denotes temperature
T2538 6558-6559 -LRB- denotes (
T2539 6559-6564 NN denotes Table
T2540 6565-6566 CD denotes 1
T2541 6566-6567 -RRB- denotes )
T2542 6568-6571 CC denotes and
T2543 6572-6573 CD denotes 1
T2544 6574-6577 NN denotes min
T2545 6578-6580 IN denotes at
T2546 6581-6583 CD denotes 94
T2547 6583-6585 NN denotes °C
T2548 6585-6586 . denotes .
T2549 6586-6718 sentence denotes Hot-measurements were performed to eliminate the detection of primer-dimers that had melting temperatures between 72 and 75°C [15].
T2550 6587-6590 JJ denotes Hot
T2552 6590-6591 HYPH denotes -
T2551 6591-6603 NNS denotes measurements
T2554 6604-6608 VBD denotes were
T2553 6609-6618 VBN denotes performed
T2555 6619-6621 TO denotes to
T2556 6622-6631 VB denotes eliminate
T2557 6632-6635 DT denotes the
T2558 6636-6645 NN denotes detection
T2559 6646-6648 IN denotes of
T2560 6649-6655 NN denotes primer
T2562 6655-6656 HYPH denotes -
T2561 6656-6662 NNS denotes dimers
T2563 6663-6667 WDT denotes that
T2564 6668-6671 VBD denotes had
T2565 6672-6679 NN denotes melting
T2566 6680-6692 NNS denotes temperatures
T2567 6693-6700 IN denotes between
T2568 6701-6703 CD denotes 72
T2570 6704-6707 CC denotes and
T2571 6708-6710 CD denotes 75
T2569 6710-6712 NN denotes °C
T2572 6713-6714 -LRB- denotes [
T2573 6714-6716 CD denotes 15
T2574 6716-6717 -RRB- denotes ]
T2575 6717-6718 . denotes .
T2576 6718-6759 sentence denotes PCR was followed by a melt (60 to 95°C).
T2577 6719-6722 NN denotes PCR
T2579 6723-6726 VBD denotes was
T2578 6727-6735 VBN denotes followed
T2580 6736-6738 IN denotes by
T2581 6739-6740 DT denotes a
T2582 6741-6745 NN denotes melt
T2583 6746-6747 -LRB- denotes (
T2585 6747-6749 CD denotes 60
T2587 6750-6752 IN denotes to
T2586 6753-6755 CD denotes 95
T2584 6755-6757 NN denotes °C
T2588 6757-6758 -RRB- denotes )
T2589 6758-6759 . denotes .
T2590 6759-6910 sentence denotes The generation of a single product of appropriate size was routinely checked by the presence of a single melt peak and by agarose gel-electrophoresis.
T2591 6760-6763 DT denotes The
T2592 6764-6774 NN denotes generation
T2594 6775-6777 IN denotes of
T2595 6778-6779 DT denotes a
T2597 6780-6786 JJ denotes single
T2596 6787-6794 NN denotes product
T2598 6795-6797 IN denotes of
T2599 6798-6809 JJ denotes appropriate
T2600 6810-6814 NN denotes size
T2601 6815-6818 VBD denotes was
T2602 6819-6828 RB denotes routinely
T2593 6829-6836 VBN denotes checked
T2603 6837-6839 IN denotes by
T2604 6840-6843 DT denotes the
T2605 6844-6852 NN denotes presence
T2606 6853-6855 IN denotes of
T2607 6856-6857 DT denotes a
T2609 6858-6864 JJ denotes single
T2610 6865-6869 NN denotes melt
T2608 6870-6874 NN denotes peak
T2611 6875-6878 CC denotes and
T2612 6879-6881 IN denotes by
T2613 6882-6889 NN denotes agarose
T2614 6890-6893 NN denotes gel
T2616 6893-6894 HYPH denotes -
T2615 6894-6909 NN denotes electrophoresis
T2617 6909-6910 . denotes .
T2618 6910-6956 sentence denotes Product identity was confirmed by sequencing.
T2619 6911-6918 NN denotes Product
T2620 6919-6927 NN denotes identity
T2622 6928-6931 VBD denotes was
T2621 6932-6941 VBN denotes confirmed
T2623 6942-6944 IN denotes by
T2624 6945-6955 NN denotes sequencing
T2625 6955-6956 . denotes .
T2626 6956-7794 sentence denotes Table 1 Primers Template Primers Product Length Melting Temperature Hot Measurement Fidelity n 18S gag gtt cga aga cga tca ga (sense) 316 bp 83.2 ± 02°C 78°C 1.89 ± 0.02 17 tcg ctc cac caa cta aga ac (antisense) KCNJ10 tgg tgt ggt gtg gta tct gg (sense) 411 bp 83.2 ± 02°C 78°C 1.86 ± 0.02 15 tga agc agt ttg cct gtc ac (antisense) KCNQ1 ttt gtt cat ccc cat ctc ag (sense) 239 bp 82.5 ± 02°C 79°C 1.85 ± 0.02 17 gtt gct ggg tag gaa gag (antisense) KCNQ4 ccc gga aac cct tct gtg tc (sense) 245 bp 83.2 ± 02°C 80°C 1.87 ± 0.01 17 aaa gat gag cac cag gaa cc (antisense) Template molecules (T) were quantified according to T = P / (F ^Ct) where P is the number of product molecules, F is the fidelity of the reaction and Ct is the cycle at which the number of product molecules reaches a chosen threshold [15].
T2627 6957-6982 NN denotes Table 1 Primers Template
T7759 6966-6973 NNS denotes Primers
T2628 7564-7573 NNS denotes molecules
T2630 7574-7575 -LRB- denotes (
T2631 7575-7576 NN denotes T
T2632 7576-7577 -RRB- denotes )
T2633 7578-7582 VBD denotes were
T2629 7583-7593 VBN denotes quantified
T2634 7594-7603 VBG denotes according
T2635 7604-7606 IN denotes to
T2636 7607-7608 NN denotes T
T2638 7609-7610 SYM denotes =
T2637 7611-7612 NN denotes P
T2639 7613-7614 SYM denotes /
T2641 7615-7616 -LRB- denotes (
T2642 7616-7617 NN denotes F
T2643 7618-7619 SYM denotes ^
T2640 7619-7621 NN denotes Ct
T2644 7621-7622 -RRB- denotes )
T2645 7623-7628 WRB denotes where
T2647 7629-7630 NN denotes P
T2646 7631-7633 VBZ denotes is
T2648 7634-7637 DT denotes the
T2649 7638-7644 NN denotes number
T2650 7645-7647 IN denotes of
T2651 7648-7655 NN denotes product
T2652 7656-7665 NNS denotes molecules
T2653 7665-7667 , denotes ,
T2654 7667-7668 NN denotes F
T2655 7669-7671 VBZ denotes is
T2656 7672-7675 DT denotes the
T2657 7676-7684 NN denotes fidelity
T2658 7685-7687 IN denotes of
T2659 7688-7691 DT denotes the
T2660 7692-7700 NN denotes reaction
T2661 7701-7704 CC denotes and
T2662 7705-7707 NN denotes Ct
T2663 7708-7710 VBZ denotes is
T2664 7711-7714 DT denotes the
T2665 7715-7720 NN denotes cycle
T2666 7721-7723 IN denotes at
T2668 7724-7729 WDT denotes which
T2669 7730-7733 DT denotes the
T2670 7734-7740 NN denotes number
T2671 7741-7743 IN denotes of
T2672 7744-7751 NN denotes product
T2673 7752-7761 NNS denotes molecules
T2667 7762-7769 VBZ denotes reaches
T2674 7770-7771 DT denotes a
T2676 7772-7778 VBN denotes chosen
T2675 7779-7788 NN denotes threshold
T2677 7789-7790 -LRB- denotes [
T2678 7790-7792 CD denotes 15
T2679 7792-7793 -RRB- denotes ]
T2680 7793-7794 . denotes .
T2681 7794-8006 sentence denotes Fidelity (F) was obtained from the slope (S) of the log-linear phase of the growth curve via a best-fit fifth-order polynomial: F = 7.39 + 3.80 × S + 1.05 × S2 + 0.15 × S3 + 11.38 × 10-3 * S4 + 3.39 × 10-4 × S5.
T2682 7795-7803 NN denotes Fidelity
T2684 7804-7805 -LRB- denotes (
T2685 7805-7806 NN denotes F
T2686 7806-7807 -RRB- denotes )
T2687 7808-7811 VBD denotes was
T2683 7812-7820 VBN denotes obtained
T2688 7821-7825 IN denotes from
T2689 7826-7829 DT denotes the
T2690 7830-7835 NN denotes slope
T2691 7836-7837 -LRB- denotes (
T2692 7837-7838 NN denotes S
T2693 7838-7839 -RRB- denotes )
T2694 7840-7842 IN denotes of
T2695 7843-7846 DT denotes the
T2697 7847-7850 NN denotes log
T2699 7850-7851 SYM denotes -
T2698 7851-7857 JJ denotes linear
T2696 7858-7863 NN denotes phase
T2700 7864-7866 IN denotes of
T2701 7867-7870 DT denotes the
T2703 7871-7877 NN denotes growth
T2702 7878-7883 NN denotes curve
T2704 7884-7887 IN denotes via
T2705 7888-7889 DT denotes a
T2707 7890-7894 JJ denotes best
T2709 7894-7895 HYPH denotes -
T2708 7895-7898 NN denotes fit
T2710 7899-7904 JJ denotes fifth
T2712 7904-7905 HYPH denotes -
T2711 7905-7910 NN denotes order
T2706 7911-7921 NN denotes polynomial
T2713 7921-7923 : denotes :
T2714 7923-7924 NN denotes F
T2716 7925-7926 SYM denotes =
T2715 7927-7931 CD denotes 7.39
T2717 7932-7933 SYM denotes +
T2718 7934-7938 CD denotes 3.80
T2720 7939-7940 SYM denotes ×
T2719 7941-7942 NN denotes S
T2721 7943-7944 SYM denotes +
T2722 7945-7949 CD denotes 1.05
T2724 7950-7951 SYM denotes ×
T2723 7952-7954 NN denotes S2
T2725 7955-7956 SYM denotes +
T2726 7957-7961 CD denotes 0.15
T2728 7962-7963 SYM denotes ×
T2727 7964-7966 NN denotes S3
T2729 7967-7968 SYM denotes +
T2730 7969-7974 CD denotes 11.38
T2732 7975-7976 SYM denotes ×
T2733 7977-7979 CD denotes 10
T2734 7979-7980 HYPH denotes -
T2731 7980-7981 CD denotes 3
T2736 7982-7983 SYM denotes *
T2735 7984-7986 NN denotes S4
T2737 7987-7988 SYM denotes +
T2738 7989-7993 CD denotes 3.39
T2740 7994-7995 SYM denotes ×
T2741 7996-7998 CD denotes 10
T2742 7998-7999 HYPH denotes -
T2739 7999-8000 CD denotes 4
T2744 8001-8002 SYM denotes ×
T2743 8003-8005 NN denotes S5
T2745 8005-8006 . denotes .
T2746 8006-8148 sentence denotes The number of product molecules at threshold (PCt) was determined by amplification of known amounts of 18S rRNA according to PCt = T × F ^Ct.
T2747 8007-8010 DT denotes The
T2748 8011-8017 NN denotes number
T2750 8018-8020 IN denotes of
T2751 8021-8028 NN denotes product
T2752 8029-8038 NNS denotes molecules
T2753 8039-8041 IN denotes at
T2754 8042-8051 NN denotes threshold
T2755 8052-8053 -LRB- denotes (
T2756 8053-8056 NN denotes PCt
T2757 8056-8057 -RRB- denotes )
T2758 8058-8061 VBD denotes was
T2749 8062-8072 VBN denotes determined
T2759 8073-8075 IN denotes by
T2760 8076-8089 NN denotes amplification
T2761 8090-8092 IN denotes of
T2762 8093-8098 VBN denotes known
T2763 8099-8106 NNS denotes amounts
T2764 8107-8109 IN denotes of
T2765 8110-8113 NN denotes 18S
T2766 8114-8118 NN denotes rRNA
T2767 8119-8128 VBG denotes according
T2768 8129-8131 IN denotes to
T2769 8132-8135 NN denotes PCt
T2771 8136-8137 SYM denotes =
T2770 8138-8139 NN denotes T
T2772 8140-8141 SYM denotes ×
T2773 8142-8143 NN denotes F
T2775 8144-8145 SYM denotes ^
T2774 8145-8147 NN denotes Ct
T2776 8147-8148 . denotes .
T2777 8148-8213 sentence denotes Quantifications of 18S rRNA were used to compare tissue amounts.
T2778 8149-8164 NNS denotes Quantifications
T2780 8165-8167 IN denotes of
T2781 8168-8171 NN denotes 18S
T2782 8172-8176 NN denotes rRNA
T2783 8177-8181 VBD denotes were
T2779 8182-8186 VBN denotes used
T2784 8187-8189 TO denotes to
T2785 8190-8197 VB denotes compare
T2786 8198-8204 NN denotes tissue
T2787 8205-8212 NNS denotes amounts
T2788 8212-8213 . denotes .
T2789 8213-8310 sentence denotes Genomic contamination of inner ear samples was assessed to be <0.02% by omission of the RT step.
T2790 8214-8221 JJ denotes Genomic
T2791 8222-8235 NN denotes contamination
T2793 8236-8238 IN denotes of
T2794 8239-8244 JJ denotes inner
T2795 8245-8248 NN denotes ear
T2796 8249-8256 NNS denotes samples
T2797 8257-8260 VBD denotes was
T2792 8261-8269 VBN denotes assessed
T2798 8270-8272 TO denotes to
T2799 8273-8275 VB denotes be
T2800 8276-8277 SYM denotes <
T2802 8277-8281 CD denotes 0.02
T2801 8281-8282 NN denotes %
T2803 8283-8285 IN denotes by
T2804 8286-8294 NN denotes omission
T2805 8295-8297 IN denotes of
T2806 8298-8301 DT denotes the
T2808 8302-8304 NN denotes RT
T2807 8305-8309 NN denotes step
T2809 8309-8310 . denotes .
T2833 8312-8314 FW denotes In
T2834 8315-8320 FW denotes vitro
T2835 8321-8338 NN denotes electrophysiology
T2836 8338-8415 sentence denotes Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.).
T2837 8339-8346 NNS denotes Animals
T2839 8347-8351 VBD denotes were
T2840 8352-8358 RB denotes deeply
T2838 8359-8371 VBN denotes anesthetized
T2841 8372-8376 IN denotes with
T2842 8377-8383 NN denotes sodium
T2843 8384-8397 NN denotes pentobarbital
T2844 8398-8399 -LRB- denotes (
T2846 8399-8402 CD denotes 100
T2845 8403-8405 NNS denotes mg
T2847 8405-8406 SYM denotes /
T2848 8406-8408 NN denotes kg
T2849 8409-8413 RB denotes i.p.
T2850 8413-8414 -RRB- denotes )
T2851 8414-8415 . denotes .
T2852 8415-8489 sentence denotes Stria vascularis without spiral ligament was obtained by microdissection.
T2853 8416-8421 NN denotes Stria
T2854 8422-8432 NN denotes vascularis
T2856 8433-8440 IN denotes without
T2857 8441-8447 JJ denotes spiral
T2858 8448-8456 NN denotes ligament
T2859 8457-8460 VBD denotes was
T2855 8461-8469 VBN denotes obtained
T2860 8470-8472 IN denotes by
T2861 8473-8488 NN denotes microdissection
T2862 8488-8489 . denotes .
T2863 8489-8561 sentence denotes Currents generated by the stria marginal epithelium were recorded [16].
T2864 8490-8498 NNS denotes Currents
T2866 8499-8508 VBN denotes generated
T2867 8509-8511 IN denotes by
T2868 8512-8515 DT denotes the
T2870 8516-8521 NN denotes stria
T2871 8522-8530 JJ denotes marginal
T2869 8531-8541 NN denotes epithelium
T2872 8542-8546 VBD denotes were
T2865 8547-8555 VBN denotes recorded
T2873 8556-8557 -LRB- denotes [
T2874 8557-8559 CD denotes 16
T2875 8559-8560 -RRB- denotes ]
T2876 8560-8561 . denotes .
T2877 8561-8833 sentence denotes A Pt-Ir wire microelectrode with a Pt-black tip was positioned 20–30 μm from the apical surface of the epithelium and vibrated at 200–400 Hz by piezo-electric bimorph elements (Applicable Electronics, Forest Dale, MA; ASET version 1.05, Science Wares, East Falmouth, MA).
T2878 8562-8563 DT denotes A
T2880 8564-8566 NN denotes Pt
T2882 8566-8567 HYPH denotes -
T2881 8567-8569 NN denotes Ir
T2883 8570-8574 NN denotes wire
T2879 8575-8589 NN denotes microelectrode
T2885 8590-8594 IN denotes with
T2886 8595-8596 DT denotes a
T2888 8597-8599 NN denotes Pt
T2889 8599-8600 HYPH denotes -
T2890 8600-8605 JJ denotes black
T2887 8606-8609 NN denotes tip
T2891 8610-8613 VBD denotes was
T2884 8614-8624 VBN denotes positioned
T2892 8625-8627 CD denotes 20
T2894 8627-8628 HYPH denotes –
T2893 8628-8630 CD denotes 30
T2895 8631-8633 NN denotes μm
T2896 8634-8638 IN denotes from
T2897 8639-8642 DT denotes the
T2899 8643-8649 JJ denotes apical
T2898 8650-8657 NN denotes surface
T2900 8658-8660 IN denotes of
T2901 8661-8664 DT denotes the
T2902 8665-8675 NN denotes epithelium
T2903 8676-8679 CC denotes and
T2904 8680-8688 VBN denotes vibrated
T2905 8689-8691 IN denotes at
T2906 8692-8695 CD denotes 200
T2908 8695-8696 HYPH denotes –
T2907 8696-8699 CD denotes 400
T2909 8700-8702 NN denotes Hz
T2910 8703-8705 IN denotes by
T2911 8706-8711 JJ denotes piezo
T2913 8711-8712 HYPH denotes -
T2912 8712-8720 JJ denotes electric
T2915 8721-8728 NN denotes bimorph
T2914 8729-8737 NNS denotes elements
T2916 8738-8739 -LRB- denotes (
T2918 8739-8749 NNP denotes Applicable
T2919 8750-8761 NNP denotes Electronics
T2920 8761-8763 , denotes ,
T2921 8763-8769 NNP denotes Forest
T2922 8770-8774 NNP denotes Dale
T2923 8774-8776 , denotes ,
T2924 8776-8778 NNP denotes MA
T2925 8778-8779 : denotes ;
T2926 8780-8784 NN denotes ASET
T2917 8785-8792 NN denotes version
T2927 8793-8797 CD denotes 1.05
T2928 8797-8799 , denotes ,
T2929 8799-8806 NNP denotes Science
T2930 8807-8812 NNP denotes Wares
T2931 8812-8814 , denotes ,
T2932 8814-8818 NNP denotes East
T2933 8819-8827 NNP denotes Falmouth
T2934 8827-8829 , denotes ,
T2935 8829-8831 NNP denotes MA
T2936 8831-8832 -RRB- denotes )
T2937 8832-8833 . denotes .
T2938 8833-8906 sentence denotes A Pt-black electrode (26-gauge) served as reference in the bath chamber.
T2939 8834-8835 DT denotes A
T2941 8836-8838 NN denotes Pt
T2942 8838-8839 HYPH denotes -
T2943 8839-8844 JJ denotes black
T2940 8845-8854 NN denotes electrode
T2945 8855-8856 -LRB- denotes (
T2947 8856-8858 CD denotes 26
T2948 8858-8859 HYPH denotes -
T2946 8859-8864 NN denotes gauge
T2949 8864-8865 -RRB- denotes )
T2944 8866-8872 VBD denotes served
T2950 8873-8875 IN denotes as
T2951 8876-8885 NN denotes reference
T2952 8886-8888 IN denotes in
T2953 8889-8892 DT denotes the
T2955 8893-8897 NN denotes bath
T2954 8898-8905 NN denotes chamber
T2956 8905-8906 . denotes .
T2957 8906-8998 sentence denotes The signals from the phase-sensitive detectors were digitized (16 bit) at a rate of 0.5 Hz.
T2958 8907-8910 DT denotes The
T2959 8911-8918 NNS denotes signals
T2961 8919-8923 IN denotes from
T2962 8924-8927 DT denotes the
T2964 8928-8933 NN denotes phase
T2966 8933-8934 HYPH denotes -
T2965 8934-8943 JJ denotes sensitive
T2963 8944-8953 NNS denotes detectors
T2967 8954-8958 VBD denotes were
T2960 8959-8968 VBN denotes digitized
T2968 8969-8970 -LRB- denotes (
T2970 8970-8972 CD denotes 16
T2969 8973-8976 NN denotes bit
T2971 8976-8977 -RRB- denotes )
T2972 8978-8980 IN denotes at
T2973 8981-8982 DT denotes a
T2974 8983-8987 NN denotes rate
T2975 8988-8990 IN denotes of
T2976 8991-8994 CD denotes 0.5
T2977 8995-8997 NN denotes Hz
T2978 8997-8998 . denotes .
T2979 8998-9060 sentence denotes The output was expressed as current density at the electrode.
T2980 8999-9002 DT denotes The
T2981 9003-9009 NN denotes output
T2983 9010-9013 VBD denotes was
T2982 9014-9023 VBN denotes expressed
T2984 9024-9026 IN denotes as
T2985 9027-9034 JJ denotes current
T2986 9035-9042 NN denotes density
T2987 9043-9045 IN denotes at
T2988 9046-9049 DT denotes the
T2989 9050-9059 NN denotes electrode
T2990 9059-9060 . denotes .
T3050 9062-9064 FW denotes In
T3051 9065-9069 FW denotes situ
T3052 9070-9087 NN denotes electrophysiology
T3053 9087-9172 sentence denotes Animals were anesthetized with inactin (thiobutabarbital sodium salt, 140 mg/kg ip).
T3054 9088-9095 NNS denotes Animals
T3056 9096-9100 VBD denotes were
T3055 9101-9113 VBN denotes anesthetized
T3057 9114-9118 IN denotes with
T3058 9119-9126 NN denotes inactin
T3059 9127-9128 -LRB- denotes (
T3061 9128-9144 NN denotes thiobutabarbital
T3063 9145-9151 NN denotes sodium
T3062 9152-9156 NN denotes salt
T3064 9156-9158 , denotes ,
T3065 9158-9161 CD denotes 140
T3060 9162-9164 NNS denotes mg
T3066 9164-9165 SYM denotes /
T3067 9165-9167 NN denotes kg
T3068 9168-9170 RB denotes ip
T3069 9170-9171 -RRB- denotes )
T3070 9171-9172 . denotes .
T3071 9172-9283 sentence denotes The endocochlear potential and the endolymphatic [K+] were measured with double-barreled microelectrodes [17].
T3072 9173-9176 DT denotes The
T3074 9177-9189 JJ denotes endocochlear
T3073 9190-9199 NN denotes potential
T3076 9200-9203 CC denotes and
T3077 9204-9207 DT denotes the
T3079 9208-9221 JJ denotes endolymphatic
T3080 9222-9223 -LRB- denotes [
T3078 9223-9224 NN denotes K
T3081 9224-9225 SYM denotes +
T3082 9225-9226 -RRB- denotes ]
T3083 9227-9231 VBD denotes were
T3075 9232-9240 VBN denotes measured
T3084 9241-9245 IN denotes with
T3085 9246-9252 JJ denotes double
T3087 9252-9253 HYPH denotes -
T3086 9253-9261 JJ denotes barreled
T3088 9262-9277 NNS denotes microelectrodes
T3089 9278-9279 -LRB- denotes [
T3090 9279-9281 CD denotes 17
T3091 9281-9282 -RRB- denotes ]
T3092 9282-9283 . denotes .
T3093 9283-9488 sentence denotes Measurements were made in the basal turn by a round-window approach through the basilar membrane and in the apical turn after thinning the bone over the stria vascularis and picking a small hole (~30 μm).
T3094 9284-9296 NNS denotes Measurements
T3096 9297-9301 VBD denotes were
T3095 9302-9306 VBN denotes made
T3097 9307-9309 IN denotes in
T3098 9310-9313 DT denotes the
T3100 9314-9319 JJ denotes basal
T3099 9320-9324 NN denotes turn
T3101 9325-9327 IN denotes by
T3102 9328-9329 DT denotes a
T3104 9330-9335 JJ denotes round
T3106 9335-9336 HYPH denotes -
T3105 9336-9342 NN denotes window
T3103 9343-9351 NN denotes approach
T3107 9352-9359 IN denotes through
T3108 9360-9363 DT denotes the
T3110 9364-9371 JJ denotes basilar
T3109 9372-9380 NN denotes membrane
T3111 9381-9384 CC denotes and
T3112 9385-9387 IN denotes in
T3113 9388-9391 DT denotes the
T3115 9392-9398 JJ denotes apical
T3114 9399-9403 NN denotes turn
T3116 9404-9409 IN denotes after
T3117 9410-9418 VBG denotes thinning
T3118 9419-9422 DT denotes the
T3119 9423-9427 NN denotes bone
T3120 9428-9432 IN denotes over
T3121 9433-9436 DT denotes the
T3123 9437-9442 NN denotes stria
T3122 9443-9453 NN denotes vascularis
T3124 9454-9457 CC denotes and
T3125 9458-9465 VBG denotes picking
T3126 9466-9467 DT denotes a
T3128 9468-9473 JJ denotes small
T3127 9474-9478 NN denotes hole
T3129 9479-9480 -LRB- denotes (
T3131 9480-9481 SYM denotes ~
T3132 9481-9483 CD denotes 30
T3130 9484-9486 NNS denotes μm
T3133 9486-9487 -RRB- denotes )
T3134 9487-9488 . denotes .
T3135 9488-9598 sentence denotes K+-selective electrodes were calibrated in solutions of constant cation (K+ and Na+) concentration of 150 mM.
T3136 9489-9490 NN denotes K
T3138 9490-9491 SYM denotes +
T3139 9491-9492 HYPH denotes -
T3137 9492-9501 JJ denotes selective
T3140 9502-9512 NNS denotes electrodes
T3142 9513-9517 VBD denotes were
T3141 9518-9528 VBN denotes calibrated
T3143 9529-9531 IN denotes in
T3144 9532-9541 NNS denotes solutions
T3145 9542-9544 IN denotes of
T3146 9545-9553 JJ denotes constant
T3148 9554-9560 NN denotes cation
T3149 9561-9562 -LRB- denotes (
T3150 9562-9563 NN denotes K
T3151 9563-9564 SYM denotes +
T3152 9565-9568 CC denotes and
T3153 9569-9571 NN denotes Na
T3154 9571-9572 SYM denotes +
T3155 9572-9573 -RRB- denotes )
T3147 9574-9587 NN denotes concentration
T3156 9588-9590 IN denotes of
T3157 9591-9594 CD denotes 150
T3158 9595-9597 NNS denotes mM
T3159 9597-9598 . denotes .
T3160 9598-9945 sentence denotes The minor selectivity of the K+ electrodes for Na+ produced a nonlinearity in the calibration curve, which was closely fitted by the Nicolski equation using nonlinear curve-fitting software (OriginLab, Northampton, MA): V = Vi + S × log ([K+] + A × [Na+]), where Vi is an offset term, S is slope, A is selectivity, and [Na+] is Na+ concentration.
T3161 9599-9602 DT denotes The
T3163 9603-9608 JJ denotes minor
T3162 9609-9620 NN denotes selectivity
T3165 9621-9623 IN denotes of
T3166 9624-9627 DT denotes the
T3168 9628-9629 NN denotes K
T3169 9629-9630 SYM denotes +
T3167 9631-9641 NNS denotes electrodes
T3170 9642-9645 IN denotes for
T3171 9646-9648 NN denotes Na
T3172 9648-9649 SYM denotes +
T3164 9650-9658 VBD denotes produced
T3173 9659-9660 DT denotes a
T3174 9661-9673 NN denotes nonlinearity
T3175 9674-9676 IN denotes in
T3176 9677-9680 DT denotes the
T3178 9681-9692 NN denotes calibration
T3177 9693-9698 NN denotes curve
T3179 9698-9700 , denotes ,
T3180 9700-9705 WDT denotes which
T3182 9706-9709 VBD denotes was
T3183 9710-9717 RB denotes closely
T3181 9718-9724 VBN denotes fitted
T3184 9725-9727 IN denotes by
T3185 9728-9731 DT denotes the
T3187 9732-9740 NNP denotes Nicolski
T3186 9741-9749 NN denotes equation
T3188 9750-9755 VBG denotes using
T3189 9756-9765 JJ denotes nonlinear
T3191 9766-9771 NN denotes curve
T3192 9771-9772 HYPH denotes -
T3193 9772-9779 VBG denotes fitting
T3190 9780-9788 NN denotes software
T3194 9789-9790 -LRB- denotes (
T3195 9790-9799 NNP denotes OriginLab
T3196 9799-9801 , denotes ,
T3197 9801-9812 NNP denotes Northampton
T3198 9812-9814 , denotes ,
T3199 9814-9816 NNP denotes MA
T3200 9816-9817 -RRB- denotes )
T3201 9817-9819 : denotes :
T3202 9819-9820 NN denotes V
T3204 9821-9822 SYM denotes =
T3205 9823-9825 NN denotes Vi
T3206 9826-9827 SYM denotes +
T3207 9828-9829 NN denotes S
T3208 9830-9831 SYM denotes ×
T3209 9832-9835 NN denotes log
T3210 9836-9837 -LRB- denotes (
T3211 9837-9838 -LRB- denotes [
T3203 9838-9839 NN denotes K
T3212 9839-9840 SYM denotes +
T3213 9840-9841 -RRB- denotes ]
T3214 9842-9843 SYM denotes +
T3215 9844-9845 NN denotes A
T3217 9846-9847 SYM denotes ×
T3218 9848-9849 -LRB- denotes [
T3216 9849-9851 NN denotes Na
T3219 9851-9852 SYM denotes +
T3220 9852-9853 -RRB- denotes ]
T3221 9853-9854 -RRB- denotes )
T3222 9854-9856 , denotes ,
T3223 9856-9861 WRB denotes where
T3225 9862-9864 NN denotes Vi
T3224 9865-9867 VBZ denotes is
T3226 9868-9870 DT denotes an
T3228 9871-9877 JJ denotes offset
T3227 9878-9882 NN denotes term
T3229 9882-9884 , denotes ,
T3230 9884-9885 NN denotes S
T3231 9886-9888 VBZ denotes is
T3232 9889-9894 NN denotes slope
T3233 9894-9896 , denotes ,
T3234 9896-9897 NN denotes A
T3235 9898-9900 VBZ denotes is
T3236 9901-9912 NN denotes selectivity
T3237 9912-9914 , denotes ,
T3238 9914-9917 CC denotes and
T3239 9918-9919 -LRB- denotes [
T3240 9919-9921 NN denotes Na
T3242 9921-9922 SYM denotes +
T3243 9922-9923 -RRB- denotes ]
T3241 9924-9926 VBZ denotes is
T3244 9927-9929 NN denotes Na
T3246 9929-9930 SYM denotes +
T3245 9931-9944 NN denotes concentration
T3247 9944-9945 . denotes .
T3248 9945-10033 sentence denotes Calibrations were made immediately after withdrawal of the electrodes from the cochlea.
T3249 9946-9958 NNS denotes Calibrations
T3251 9959-9963 VBD denotes were
T3250 9964-9968 VBN denotes made
T3252 9969-9980 RB denotes immediately
T3253 9981-9986 IN denotes after
T3254 9987-9997 NN denotes withdrawal
T3255 9998-10000 IN denotes of
T3256 10001-10004 DT denotes the
T3257 10005-10015 NNS denotes electrodes
T3258 10016-10020 IN denotes from
T3259 10021-10024 DT denotes the
T3260 10025-10032 NN denotes cochlea
T3261 10032-10033 . denotes .
T3262 10033-10143 sentence denotes Plasma K+ concentrations were obtained using a blood analyzer (Stat Profile M, Nova Biomedical, Waltham, MA).
T3263 10034-10040 NN denotes Plasma
T3265 10041-10042 NN denotes K
T3266 10042-10043 SYM denotes +
T3264 10044-10058 NNS denotes concentrations
T3268 10059-10063 VBD denotes were
T3267 10064-10072 VBN denotes obtained
T3269 10073-10078 VBG denotes using
T3270 10079-10080 DT denotes a
T3272 10081-10086 NN denotes blood
T3271 10087-10095 NN denotes analyzer
T3273 10096-10097 -LRB- denotes (
T3275 10097-10101 NNP denotes Stat
T3276 10102-10109 NNP denotes Profile
T3274 10110-10111 NNP denotes M
T3277 10111-10113 , denotes ,
T3278 10113-10117 NNP denotes Nova
T3279 10118-10128 NNP denotes Biomedical
T3280 10128-10130 , denotes ,
T3281 10130-10137 NNP denotes Waltham
T3282 10137-10139 , denotes ,
T3283 10139-10141 NNP denotes MA
T3284 10141-10142 -RRB- denotes )
T3285 10142-10143 . denotes .
T3286 10143-10214 sentence denotes Data are presented as mean ± sem; n denotes the number of experiments.
T3287 10144-10148 NNS denotes Data
T3289 10149-10152 VBP denotes are
T3288 10153-10162 VBN denotes presented
T3291 10163-10165 IN denotes as
T3292 10166-10170 NN denotes mean
T3294 10171-10172 NN denotes ±
T3293 10173-10176 NN denotes sem
T3295 10176-10177 : denotes ;
T3296 10178-10179 NN denotes n
T3290 10180-10187 VBZ denotes denotes
T3297 10188-10191 DT denotes the
T3298 10192-10198 NN denotes number
T3299 10199-10201 IN denotes of
T3300 10202-10213 NNS denotes experiments
T3301 10213-10214 . denotes .
T3302 10214-10269 sentence denotes Differences were considered significant when p < 0.05.
T3303 10215-10226 NNS denotes Differences
T3305 10227-10231 VBD denotes were
T3304 10232-10242 VBN denotes considered
T3306 10243-10254 JJ denotes significant
T3307 10255-10259 WRB denotes when
T3309 10260-10261 NN denotes p
T3310 10262-10263 SYM denotes <
T3308 10264-10268 CD denotes 0.05
T3311 10268-10269 . denotes .
T4701 10294-10296 FW denotes In
T4702 10297-10301 FW denotes situ
T4703 10302-10315 NN denotes hybridization
T4705 10316-10318 IN denotes in
T4706 10319-10322 DT denotes the
T4707 10323-10330 NN denotes cochlea
T4708 10331-10334 VBZ denotes has
T4704 10335-10344 VBN denotes suggested
T4709 10345-10349 IN denotes that
T4711 10350-10357 NN denotes pendrin
T4712 10358-10362 NN denotes mRNA
T4713 10363-10365 VBZ denotes is
T4710 10366-10375 VBN denotes expressed
T4714 10376-10378 IN denotes in
T4715 10379-10384 NNS denotes cells
T4716 10385-10389 WDT denotes that
T4717 10390-10396 VBP denotes reside
T4718 10397-10408 RB denotes immediately
T4719 10409-10416 IN denotes beneath
T4720 10417-10420 DT denotes the
T4722 10421-10427 JJ denotes spiral
T4721 10428-10438 NN denotes prominence
T4723 10439-10441 IN denotes on
T4724 10442-10445 DT denotes the
T4726 10446-10453 JJ denotes lateral
T4725 10454-10458 NN denotes wall
T4727 10459-10461 IN denotes of
T4728 10462-10465 DT denotes the
T4730 10466-10474 JJ denotes external
T4729 10475-10481 NN denotes sulcus
T4731 10482-10483 -LRB- denotes [
T4732 10483-10484 CD denotes 8
T4733 10484-10485 -RRB- denotes ]
T4734 10485-10486 . denotes .
T4735 10486-10693 sentence denotes To determine the location of pendrin protein expression, we performed confocal immunocytochemistry on cryosections prepared from temporal bones of normal (Slc26a4+/+) mice using an established antibody [3].
T4736 10487-10489 TO denotes To
T4737 10490-10499 VB denotes determine
T4739 10500-10503 DT denotes the
T4740 10504-10512 NN denotes location
T4741 10513-10515 IN denotes of
T4742 10516-10523 NN denotes pendrin
T4744 10524-10531 NN denotes protein
T4743 10532-10542 NN denotes expression
T4745 10542-10544 , denotes ,
T4746 10544-10546 PRP denotes we
T4738 10547-10556 VBD denotes performed
T4747 10557-10565 JJ denotes confocal
T4748 10566-10585 NN denotes immunocytochemistry
T4749 10586-10588 IN denotes on
T4750 10589-10601 NNS denotes cryosections
T4751 10602-10610 VBN denotes prepared
T4752 10611-10615 IN denotes from
T4753 10616-10624 JJ denotes temporal
T4754 10625-10630 NNS denotes bones
T4755 10631-10633 IN denotes of
T4756 10634-10640 JJ denotes normal
T4758 10641-10642 -LRB- denotes (
T4759 10642-10649 NN denotes Slc26a4
T4760 10649-10650 SYM denotes +
T4761 10650-10651 HYPH denotes /
T4762 10651-10652 SYM denotes +
T4763 10652-10653 -RRB- denotes )
T4757 10654-10658 NNS denotes mice
T4764 10659-10664 VBG denotes using
T4765 10665-10667 DT denotes an
T4767 10668-10679 JJ denotes established
T4766 10680-10688 NN denotes antibody
T4768 10689-10690 -LRB- denotes [
T4769 10690-10691 CD denotes 3
T4770 10691-10692 -RRB- denotes ]
T4771 10692-10693 . denotes .
T4772 10693-10801 sentence denotes Staining was absent when the primary antibody was pre-absorbed with the antigenic peptide (data not shown).
T4773 10694-10702 NN denotes Staining
T4774 10703-10706 VBD denotes was
T4775 10707-10713 JJ denotes absent
T4776 10714-10718 WRB denotes when
T4778 10719-10722 DT denotes the
T4780 10723-10730 JJ denotes primary
T4779 10731-10739 NN denotes antibody
T4781 10740-10743 VBD denotes was
T4777 10744-10756 VBN denotes pre-absorbed
T4782 10757-10761 IN denotes with
T4783 10762-10765 DT denotes the
T4785 10766-10775 JJ denotes antigenic
T4784 10776-10783 NN denotes peptide
T4786 10784-10785 -LRB- denotes (
T4788 10785-10789 NNS denotes data
T4789 10790-10793 RB denotes not
T4787 10794-10799 VBN denotes shown
T4790 10799-10800 -RRB- denotes )
T4791 10800-10801 . denotes .
T4792 10801-11121 sentence denotes Strong expression of pendrin was observed not only in outer sulcus epithelial cells, as predicted from in situ hybridization data, but also in root cells, in apical membranes of spiral prominence surface epithelial cells and in apical membranes of spindle-shaped cells that are part of stria vascularis (Fig. 1a,1b,1c).
T4793 10802-10808 JJ denotes Strong
T4794 10809-10819 NN denotes expression
T4796 10820-10822 IN denotes of
T4797 10823-10830 NN denotes pendrin
T4798 10831-10834 VBD denotes was
T4795 10835-10843 VBN denotes observed
T4799 10844-10847 RB denotes not
T4801 10848-10852 RB denotes only
T4800 10853-10855 IN denotes in
T4802 10856-10861 JJ denotes outer
T4803 10862-10868 NN denotes sulcus
T4805 10869-10879 JJ denotes epithelial
T4804 10880-10885 NNS denotes cells
T4806 10885-10887 , denotes ,
T4808 10887-10889 IN denotes as
T4807 10890-10899 VBN denotes predicted
T4809 10900-10904 IN denotes from
T4810 10905-10907 FW denotes in
T4811 10908-10912 FW denotes situ
T4813 10913-10926 NN denotes hybridization
T4812 10927-10931 NNS denotes data
T4814 10931-10933 , denotes ,
T4815 10933-10936 CC denotes but
T4816 10937-10941 RB denotes also
T4817 10942-10944 IN denotes in
T4818 10945-10949 NN denotes root
T4819 10950-10955 NNS denotes cells
T4820 10955-10957 , denotes ,
T4821 10957-10959 IN denotes in
T4822 10960-10966 JJ denotes apical
T4823 10967-10976 NNS denotes membranes
T4824 10977-10979 IN denotes of
T4825 10980-10986 JJ denotes spiral
T4826 10987-10997 NN denotes prominence
T4828 10998-11005 NN denotes surface
T4829 11006-11016 JJ denotes epithelial
T4827 11017-11022 NNS denotes cells
T4830 11023-11026 CC denotes and
T4831 11027-11029 IN denotes in
T4832 11030-11036 JJ denotes apical
T4833 11037-11046 NNS denotes membranes
T4834 11047-11049 IN denotes of
T4835 11050-11057 NN denotes spindle
T4837 11057-11058 HYPH denotes -
T4836 11058-11064 VBN denotes shaped
T4838 11065-11070 NNS denotes cells
T4839 11071-11075 WDT denotes that
T4840 11076-11079 VBP denotes are
T4841 11080-11084 NN denotes part
T4842 11085-11087 IN denotes of
T4843 11088-11093 NN denotes stria
T4844 11094-11104 NN denotes vascularis
T4845 11105-11106 -LRB- denotes (
T4847 11106-11110 NN denotes Fig.
T4848 11111-11113 CD denotes 1a
T4849 11113-11114 , denotes ,
T4850 11114-11116 CD denotes 1b
T4851 11116-11117 , denotes ,
T4846 11117-11119 CD denotes 1c
T4852 11119-11120 -RRB- denotes )
T4853 11120-11121 . denotes .
T4854 11121-11226 sentence denotes The presence of pendrin in spindle-shaped cells suggests that these cells secrete HCO3 - into endolymph.
T4855 11122-11125 DT denotes The
T4856 11126-11134 NN denotes presence
T4858 11135-11137 IN denotes of
T4859 11138-11145 NN denotes pendrin
T4860 11146-11148 IN denotes in
T4861 11149-11156 NN denotes spindle
T4863 11156-11157 HYPH denotes -
T4862 11157-11163 VBN denotes shaped
T4864 11164-11169 NNS denotes cells
T4857 11170-11178 VBZ denotes suggests
T4865 11179-11183 IN denotes that
T4867 11184-11189 DT denotes these
T4868 11190-11195 NNS denotes cells
T4866 11196-11203 VBP denotes secrete
T4869 11204-11208 NN denotes HCO3
T4870 11209-11210 SYM denotes -
T4871 11211-11215 IN denotes into
T4872 11216-11225 NN denotes endolymph
T4873 11225-11226 . denotes .
T4874 11226-11305 sentence denotes Pendrin-mediated HCO3 - transport has previously been shown in the kidney [9].
T4875 11227-11234 NN denotes Pendrin
T4877 11234-11235 HYPH denotes -
T4876 11235-11243 VBN denotes mediated
T4879 11244-11248 NN denotes HCO3
T4880 11249-11250 SYM denotes -
T4878 11251-11260 NN denotes transport
T4882 11261-11264 VBZ denotes has
T4883 11265-11275 RB denotes previously
T4884 11276-11280 VBN denotes been
T4881 11281-11286 VBN denotes shown
T4885 11287-11289 IN denotes in
T4886 11290-11293 DT denotes the
T4887 11294-11300 NN denotes kidney
T4888 11301-11302 -LRB- denotes [
T4889 11302-11303 CD denotes 9
T4890 11303-11304 -RRB- denotes ]
T4891 11304-11305 . denotes .
T4892 11305-11421 sentence denotes HCO3 - in the cochlea may be generated from CO2 by carbonic anhydrase located in strial intermediate cells [18,19].
T4893 11306-11310 NN denotes HCO3
T4895 11311-11312 SYM denotes -
T4896 11313-11315 IN denotes in
T4897 11316-11319 DT denotes the
T4898 11320-11327 NN denotes cochlea
T4899 11328-11331 MD denotes may
T4900 11332-11334 VB denotes be
T4894 11335-11344 VBN denotes generated
T4901 11345-11349 IN denotes from
T4902 11350-11353 NN denotes CO2
T4903 11354-11356 IN denotes by
T4904 11357-11365 JJ denotes carbonic
T4905 11366-11375 NN denotes anhydrase
T4906 11376-11383 VBN denotes located
T4907 11384-11386 IN denotes in
T4908 11387-11393 JJ denotes strial
T4910 11394-11406 JJ denotes intermediate
T4909 11407-11412 NNS denotes cells
T4911 11413-11414 -LRB- denotes [
T4913 11414-11416 CD denotes 18
T4914 11416-11417 , denotes ,
T4912 11417-11419 CD denotes 19
T4915 11419-11420 -RRB- denotes ]
T4916 11420-11421 . denotes .
T4917 11421-11498 sentence denotes CO2 may be supplied by the metabolically highly active stria marginal cells.
T4918 11422-11425 NN denotes CO2
T4920 11426-11429 MD denotes may
T4921 11430-11432 VB denotes be
T4919 11433-11441 VBN denotes supplied
T4922 11442-11444 IN denotes by
T4923 11445-11448 DT denotes the
T4925 11449-11462 RB denotes metabolically
T4927 11463-11469 RB denotes highly
T4926 11470-11476 JJ denotes active
T4928 11477-11482 NN denotes stria
T4929 11483-11491 JJ denotes marginal
T4924 11492-11497 NNS denotes cells
T4930 11497-11498 . denotes .
T4931 11498-11629 sentence denotes It is conceivable that pendrin dysfunction interrupts HCO3 - secretion and leads to an accumulation of HCO3 - in stria vascularis.
T4932 11499-11501 PRP denotes It
T4933 11502-11504 VBZ denotes is
T4934 11505-11516 JJ denotes conceivable
T4935 11517-11521 IN denotes that
T4937 11522-11529 NN denotes pendrin
T4938 11530-11541 NN denotes dysfunction
T4936 11542-11552 VBZ denotes interrupts
T4939 11553-11557 NN denotes HCO3
T4941 11558-11559 SYM denotes -
T4940 11560-11569 NN denotes secretion
T4942 11570-11573 CC denotes and
T4943 11574-11579 VBZ denotes leads
T4944 11580-11582 IN denotes to
T4945 11583-11585 DT denotes an
T4946 11586-11598 NN denotes accumulation
T4947 11599-11601 IN denotes of
T4948 11602-11606 NN denotes HCO3
T4949 11607-11608 SYM denotes -
T4950 11609-11611 IN denotes in
T4951 11612-11617 NN denotes stria
T4952 11618-11628 NN denotes vascularis
T4953 11628-11629 . denotes .
T4954 11629-11741 sentence denotes Preliminary data (Wangemann et al., unpublished) support a role for pendrin in HCO3 - secretion into endolymph.
T4955 11630-11641 JJ denotes Preliminary
T4956 11642-11646 NNS denotes data
T4958 11647-11648 -LRB- denotes (
T4959 11648-11657 NNP denotes Wangemann
T4960 11658-11660 FW denotes et
T4961 11661-11664 FW denotes al.
T4962 11664-11666 , denotes ,
T4963 11666-11677 JJ denotes unpublished
T4964 11677-11678 -RRB- denotes )
T4957 11679-11686 VBP denotes support
T4965 11687-11688 DT denotes a
T4966 11689-11693 NN denotes role
T4967 11694-11697 IN denotes for
T4968 11698-11705 NN denotes pendrin
T4969 11706-11708 IN denotes in
T4970 11709-11713 NN denotes HCO3
T4972 11714-11715 SYM denotes -
T4971 11716-11725 NN denotes secretion
T4973 11726-11730 IN denotes into
T4974 11731-11740 NN denotes endolymph
T4975 11740-11741 . denotes .
T4976 11741-11742 sentence denotes
T7896 11752-11759 NN denotes Protein
T7897 11760-11772 NN denotes localization
T7898 11773-11775 IN denotes of
T7899 11776-11783 NN denotes pendrin
T7900 11783-11785 , denotes ,
T7901 11785-11790 NN denotes KCNQ1
T7902 11790-11792 , denotes ,
T7903 11792-11794 NN denotes ZO
T7904 11794-11795 HYPH denotes -
T7905 11795-11796 CD denotes 1
T7906 11797-11800 CC denotes and
T7907 11801-11802 NN denotes F
T7908 11803-11808 NN denotes actin
T7909 11809-11811 IN denotes in
T7910 11812-11819 NN denotes cochlea
T7911 11820-11823 CC denotes and
T7912 11824-11834 JJ denotes vestibular
T7913 11835-11844 NN denotes labyrinth
T7914 11845-11847 IN denotes of
T7915 11848-11855 NN denotes Slc26a4
T7917 11855-11856 SYM denotes +
T7918 11856-11857 HYPH denotes /
T7919 11857-11858 SYM denotes +
T7920 11859-11862 CC denotes and
T7921 11863-11870 NN denotes Slc26a4
T7922 11870-11871 SYM denotes -
T7923 11871-11872 HYPH denotes /
T7924 11872-11873 SYM denotes -
T7916 11874-11878 NNS denotes mice
T7925 11878-11879 . denotes .
T7926 11879-11917 sentence denotes a: Overview of cochlea; bar = 100 μm.
T7927 11880-11881 LS denotes a
T7929 11881-11883 : denotes :
T7930 11883-11891 NN denotes Overview
T7931 11892-11894 IN denotes of
T7932 11895-11902 NN denotes cochlea
T7933 11902-11903 : denotes ;
T7934 11904-11907 NN denotes bar
T7935 11908-11909 SYM denotes =
T7936 11910-11913 CD denotes 100
T7928 11914-11916 NNS denotes μm
T7937 11916-11917 . denotes .
T7938 11917-11968 sentence denotes b-f: Detail of cochlear lateral wall; bar = 10 μm.
T7939 11918-11919 LS denotes b
T7941 11919-11920 HYPH denotes -
T7942 11920-11921 LS denotes f
T7943 11921-11923 : denotes :
T7944 11923-11929 NN denotes Detail
T7945 11930-11932 IN denotes of
T7946 11933-11941 JJ denotes cochlear
T7948 11942-11949 JJ denotes lateral
T7947 11950-11954 NN denotes wall
T7949 11954-11955 : denotes ;
T7950 11956-11959 NN denotes bar
T7951 11960-11961 SYM denotes =
T7952 11962-11964 CD denotes 10
T7940 11965-11967 NNS denotes μm
T7953 11967-11968 . denotes .
T7954 11968-12003 sentence denotes g: Detail of utricle; bar = 10 μm.
T7955 11969-11970 LS denotes g
T7957 11970-11972 : denotes :
T7958 11972-11978 NN denotes Detail
T7959 11979-11981 IN denotes of
T7960 11982-11989 NN denotes utricle
T7961 11989-11990 : denotes ;
T7962 11991-11994 NN denotes bar
T7963 11995-11996 SYM denotes =
T7964 11997-11999 CD denotes 10
T7956 12000-12002 NNS denotes μm
T7965 12002-12003 . denotes .
T7966 12003-12040 sentence denotes h-i: Detail of ampulla; bar = 10 μm.
T7967 12004-12005 LS denotes h
T7969 12005-12006 HYPH denotes -
T7970 12006-12007 LS denotes i
T7971 12007-12009 : denotes :
T7972 12009-12015 NN denotes Detail
T7973 12016-12018 IN denotes of
T7974 12019-12026 NN denotes ampulla
T7975 12026-12027 : denotes ;
T7976 12028-12031 NN denotes bar
T7977 12032-12033 SYM denotes =
T7978 12034-12036 CD denotes 10
T7968 12037-12039 NNS denotes μm
T7979 12039-12040 . denotes .
T7980 12040-12182 sentence denotes RM, Reissner's membrane; SC, spindle-shaped cells, SMC, strial marginal cells; SV, stria vascularis; SL, spiral ligament; LIM, spiral limbus.
T7981 12041-12043 NN denotes RM
T7982 12043-12045 , denotes ,
T7983 12045-12053 NNP denotes Reissner
T7985 12053-12055 POS denotes 's
T7984 12056-12064 NN denotes membrane
T7986 12064-12065 , denotes ;
T7987 12066-12068 NN denotes SC
T7988 12068-12070 , denotes ,
T7989 12070-12077 NN denotes spindle
T7991 12077-12078 HYPH denotes -
T7990 12078-12084 VBN denotes shaped
T7992 12085-12090 NNS denotes cells
T7993 12090-12092 , denotes ,
T7994 12092-12095 NN denotes SMC
T7995 12095-12097 , denotes ,
T7996 12097-12103 JJ denotes strial
T7997 12104-12112 JJ denotes marginal
T7998 12113-12118 NNS denotes cells
T7999 12118-12119 , denotes ;
T8000 12120-12122 NN denotes SV
T8001 12122-12124 , denotes ,
T8002 12124-12129 NN denotes stria
T8003 12130-12140 NN denotes vascularis
T8004 12140-12141 , denotes ;
T8005 12142-12144 NN denotes SL
T8006 12144-12146 , denotes ,
T8007 12146-12152 JJ denotes spiral
T8008 12153-12161 NN denotes ligament
T8009 12161-12162 , denotes ;
T8010 12163-12166 NN denotes LIM
T8011 12166-12168 , denotes ,
T8012 12168-12174 JJ denotes spiral
T8013 12175-12181 NN denotes limbus
T8014 12181-12182 . denotes .
T8015 12182-12496 sentence denotes BC, basal cells; SP, spiral prominence epithelial cells; RC, root cells; OS, outer sulcus epithelial cells; VHC, vestibular hair cells; VTC, vestibular transitional cells; VDC, vestibular dark cells; arrows, basal cells at the top and bottom of stria vascularis form tight junctions with surface epithelial cells.
T8016 12183-12185 NN denotes BC
T8017 12185-12187 , denotes ,
T8018 12187-12192 JJ denotes basal
T8019 12193-12198 NNS denotes cells
T8020 12198-12199 , denotes ;
T8021 12200-12202 NN denotes SP
T8022 12202-12204 , denotes ,
T8023 12204-12210 JJ denotes spiral
T8024 12211-12221 NN denotes prominence
T8026 12222-12232 JJ denotes epithelial
T8025 12233-12238 NNS denotes cells
T8027 12238-12239 , denotes ;
T8028 12240-12242 NN denotes RC
T8029 12242-12244 , denotes ,
T8030 12244-12248 NN denotes root
T8031 12249-12254 NNS denotes cells
T8032 12254-12255 , denotes ;
T8033 12256-12258 NN denotes OS
T8034 12258-12260 , denotes ,
T8035 12260-12265 JJ denotes outer
T8036 12266-12272 NN denotes sulcus
T8038 12273-12283 JJ denotes epithelial
T8037 12284-12289 NNS denotes cells
T8039 12289-12290 , denotes ;
T8040 12291-12294 NN denotes VHC
T8041 12294-12296 , denotes ,
T8042 12296-12306 JJ denotes vestibular
T8044 12307-12311 NN denotes hair
T8043 12312-12317 NNS denotes cells
T8045 12317-12318 , denotes ;
T8046 12319-12322 NN denotes VTC
T8047 12322-12324 , denotes ,
T8048 12324-12334 JJ denotes vestibular
T8050 12335-12347 JJ denotes transitional
T8049 12348-12353 NNS denotes cells
T8051 12353-12354 , denotes ;
T8052 12355-12358 NN denotes VDC
T8053 12358-12360 , denotes ,
T8054 12360-12370 JJ denotes vestibular
T8056 12371-12375 JJ denotes dark
T8055 12376-12381 NNS denotes cells
T8057 12381-12382 , denotes ;
T8058 12383-12389 NNS denotes arrows
T8060 12389-12391 , denotes ,
T8061 12391-12396 JJ denotes basal
T8062 12397-12402 NNS denotes cells
T8063 12403-12405 IN denotes at
T8064 12406-12409 DT denotes the
T8065 12410-12413 NN denotes top
T8066 12414-12417 CC denotes and
T8067 12418-12424 NN denotes bottom
T8068 12425-12427 IN denotes of
T8069 12428-12433 NN denotes stria
T8070 12434-12444 NN denotes vascularis
T8059 12445-12449 VBP denotes form
T8071 12450-12455 JJ denotes tight
T8072 12456-12465 NNS denotes junctions
T8073 12466-12470 IN denotes with
T8074 12471-12478 NN denotes surface
T8076 12479-12489 JJ denotes epithelial
T8075 12490-12495 NNS denotes cells
T8077 12495-12496 . denotes .
T4978 12497-12498 NN denotes T
T4977 12497-12502 sentence denotes The e
T4979 12498-12500 DT denotes he
T4980 12501-12502 NN denotes e
T4982 12502-12507 NN denotes xtent
T4981 12502-12786 sentence denotes xtent of pendrin expression in spiral prominence cells and stria vascularis was determined by labeling KCNQ1, a K+ channel that is expressed in strial marginal cells, and ZO-1, a tight junction protein that labels basal cells and thereby delineates the boundaries of stria vascularis.
T4984 12508-12510 IN denotes of
T4985 12511-12518 NN denotes pendrin
T4986 12519-12529 NN denotes expression
T4987 12530-12532 IN denotes in
T4988 12533-12539 JJ denotes spiral
T4990 12540-12550 NN denotes prominence
T4989 12551-12556 NNS denotes cells
T4991 12557-12560 CC denotes and
T4992 12561-12566 NN denotes stria
T4993 12567-12577 NN denotes vascularis
T4994 12578-12581 VBD denotes was
T4983 12582-12592 VBN denotes determined
T4995 12593-12595 IN denotes by
T4996 12596-12604 VBG denotes labeling
T4997 12605-12610 NN denotes KCNQ1
T4998 12610-12612 , denotes ,
T4999 12612-12613 DT denotes a
T5001 12614-12615 NN denotes K
T5002 12615-12616 SYM denotes +
T5000 12617-12624 NN denotes channel
T5003 12625-12629 WDT denotes that
T5005 12630-12632 VBZ denotes is
T5004 12633-12642 VBN denotes expressed
T5006 12643-12645 IN denotes in
T5007 12646-12652 JJ denotes strial
T5008 12653-12661 JJ denotes marginal
T5009 12662-12667 NNS denotes cells
T5010 12667-12669 , denotes ,
T5011 12669-12672 CC denotes and
T5012 12673-12675 NN denotes ZO
T5013 12675-12676 HYPH denotes -
T5014 12676-12677 CD denotes 1
T5015 12677-12679 , denotes ,
T5016 12679-12680 DT denotes a
T5018 12681-12686 JJ denotes tight
T5019 12687-12695 NN denotes junction
T5017 12696-12703 NN denotes protein
T5020 12704-12708 WDT denotes that
T5021 12709-12715 VBZ denotes labels
T5022 12716-12721 JJ denotes basal
T5023 12722-12727 NNS denotes cells
T5024 12728-12731 CC denotes and
T5025 12732-12739 RB denotes thereby
T5026 12740-12750 VBZ denotes delineates
T5027 12751-12754 DT denotes the
T5028 12755-12765 NNS denotes boundaries
T5029 12766-12768 IN denotes of
T5030 12769-12774 NN denotes stria
T5031 12775-12785 NN denotes vascularis
T5032 12785-12786 . denotes .
T5033 12786-13047 sentence denotes Dual labeling experiments demonstrated that pendrin is expressed in spindle-shaped cells, which are surface epithelial cells in stria vascularis adjacent to strial marginal cells near the borders of both the spiral prominence and Reissner's membrane (Fig. 1c).
T5034 12787-12791 JJ denotes Dual
T5036 12792-12800 NN denotes labeling
T5035 12801-12812 NNS denotes experiments
T5037 12813-12825 VBD denotes demonstrated
T5038 12826-12830 IN denotes that
T5040 12831-12838 NN denotes pendrin
T5041 12839-12841 VBZ denotes is
T5039 12842-12851 VBN denotes expressed
T5042 12852-12854 IN denotes in
T5043 12855-12862 NN denotes spindle
T5045 12862-12863 HYPH denotes -
T5044 12863-12869 VBN denotes shaped
T5046 12870-12875 NNS denotes cells
T5047 12875-12877 , denotes ,
T5048 12877-12882 WDT denotes which
T5049 12883-12886 VBP denotes are
T5050 12887-12894 JJ denotes surface
T5052 12895-12905 JJ denotes epithelial
T5051 12906-12911 NNS denotes cells
T5053 12912-12914 IN denotes in
T5054 12915-12920 NN denotes stria
T5055 12921-12931 NN denotes vascularis
T5056 12932-12940 JJ denotes adjacent
T5057 12941-12943 IN denotes to
T5058 12944-12950 JJ denotes strial
T5060 12951-12959 JJ denotes marginal
T5059 12960-12965 NNS denotes cells
T5061 12966-12970 IN denotes near
T5062 12971-12974 DT denotes the
T5063 12975-12982 NNS denotes borders
T5064 12983-12985 IN denotes of
T5065 12986-12990 CC denotes both
T5067 12991-12994 DT denotes the
T5068 12995-13001 JJ denotes spiral
T5066 13002-13012 NN denotes prominence
T5069 13013-13016 CC denotes and
T5070 13017-13025 NNP denotes Reissner
T5072 13025-13027 POS denotes 's
T5071 13028-13036 NN denotes membrane
T5073 13037-13038 -LRB- denotes (
T5074 13038-13042 NN denotes Fig.
T5075 13043-13045 CD denotes 1c
T5076 13045-13046 -RRB- denotes )
T5077 13046-13047 . denotes .
T5078 13047-13164 sentence denotes In situ hybridization in the vestibular labyrinth suggested that pendrin mRNA is expressed in non-sensory cells [8].
T5079 13048-13050 FW denotes In
T5080 13051-13055 FW denotes situ
T5081 13056-13069 NN denotes hybridization
T5083 13070-13072 IN denotes in
T5084 13073-13076 DT denotes the
T5086 13077-13087 JJ denotes vestibular
T5085 13088-13097 NN denotes labyrinth
T5082 13098-13107 VBD denotes suggested
T5087 13108-13112 IN denotes that
T5089 13113-13120 NN denotes pendrin
T5090 13121-13125 NN denotes mRNA
T5091 13126-13128 VBZ denotes is
T5088 13129-13138 VBN denotes expressed
T5092 13139-13141 IN denotes in
T5093 13142-13153 JJ denotes non-sensory
T5094 13154-13159 NNS denotes cells
T5095 13160-13161 -LRB- denotes [
T5096 13161-13162 CD denotes 8
T5097 13162-13163 -RRB- denotes ]
T5098 13163-13164 . denotes .
T5099 13164-13370 sentence denotes Using confocal immunocytochemistry on cryosections, we observed strong expression of the pendrin protein in the apical membrane of vestibular transitional cells in the utricle and ampullae (Fig. 1g,1h,1i).
T5100 13165-13170 VBG denotes Using
T5102 13171-13179 JJ denotes confocal
T5103 13180-13199 NN denotes immunocytochemistry
T5104 13200-13202 IN denotes on
T5105 13203-13215 NNS denotes cryosections
T5106 13215-13217 , denotes ,
T5107 13217-13219 PRP denotes we
T5101 13220-13228 VBD denotes observed
T5108 13229-13235 JJ denotes strong
T5109 13236-13246 NN denotes expression
T5110 13247-13249 IN denotes of
T5111 13250-13253 DT denotes the
T5113 13254-13261 NN denotes pendrin
T5112 13262-13269 NN denotes protein
T5114 13270-13272 IN denotes in
T5115 13273-13276 DT denotes the
T5117 13277-13283 JJ denotes apical
T5116 13284-13292 NN denotes membrane
T5118 13293-13295 IN denotes of
T5119 13296-13306 JJ denotes vestibular
T5121 13307-13319 JJ denotes transitional
T5120 13320-13325 NNS denotes cells
T5122 13326-13328 IN denotes in
T5123 13329-13332 DT denotes the
T5124 13333-13340 NN denotes utricle
T5125 13341-13344 CC denotes and
T5126 13345-13353 NNS denotes ampullae
T5127 13354-13355 -LRB- denotes (
T5129 13355-13359 NN denotes Fig.
T5130 13360-13362 CD denotes 1g
T5131 13362-13363 , denotes ,
T5132 13363-13365 CD denotes 1h
T5133 13365-13366 , denotes ,
T5128 13366-13368 CD denotes 1i
T5134 13368-13369 -RRB- denotes )
T5135 13369-13370 . denotes .
T5136 13370-13656 sentence denotes Dual labeling with KCNQ1 demonstrated that pendrin expression was clearly limited to vestibular transitional cells and did not extend to other non-sensory cells such as vestibular dark cells, which were clearly identified by the expression of KCNQ1 and KCNE1 in their apical membranes.
T5137 13371-13375 JJ denotes Dual
T5138 13376-13384 NN denotes labeling
T5140 13385-13389 IN denotes with
T5141 13390-13395 NN denotes KCNQ1
T5139 13396-13408 VBD denotes demonstrated
T5142 13409-13413 IN denotes that
T5144 13414-13421 NN denotes pendrin
T5145 13422-13432 NN denotes expression
T5146 13433-13436 VBD denotes was
T5147 13437-13444 RB denotes clearly
T5143 13445-13452 VBN denotes limited
T5148 13453-13455 IN denotes to
T5149 13456-13466 JJ denotes vestibular
T5151 13467-13479 JJ denotes transitional
T5150 13480-13485 NNS denotes cells
T5152 13486-13489 CC denotes and
T5153 13490-13493 VBD denotes did
T5155 13494-13497 RB denotes not
T5154 13498-13504 VB denotes extend
T5156 13505-13507 IN denotes to
T5157 13508-13513 JJ denotes other
T5159 13514-13525 JJ denotes non-sensory
T5158 13526-13531 NNS denotes cells
T5160 13532-13536 JJ denotes such
T5161 13537-13539 IN denotes as
T5162 13540-13550 JJ denotes vestibular
T5164 13551-13555 JJ denotes dark
T5163 13556-13561 NNS denotes cells
T5165 13561-13563 , denotes ,
T5166 13563-13568 WDT denotes which
T5168 13569-13573 VBD denotes were
T5169 13574-13581 RB denotes clearly
T5167 13582-13592 VBN denotes identified
T5170 13593-13595 IN denotes by
T5171 13596-13599 DT denotes the
T5172 13600-13610 NN denotes expression
T5173 13611-13613 IN denotes of
T5174 13614-13619 NN denotes KCNQ1
T5175 13620-13623 CC denotes and
T5176 13624-13629 NN denotes KCNE1
T5177 13630-13632 IN denotes in
T5178 13633-13638 PRP$ denotes their
T5180 13639-13645 JJ denotes apical
T5179 13646-13655 NNS denotes membranes
T5181 13655-13656 . denotes .
T5182 13656-13784 sentence denotes The onset of pendrin expression during development of the mouse inner ear has been determined to be embryonic day (ED) 13 [14].
T5183 13657-13660 DT denotes The
T5184 13661-13666 NN denotes onset
T5186 13667-13669 IN denotes of
T5187 13670-13677 NN denotes pendrin
T5188 13678-13688 NN denotes expression
T5189 13689-13695 IN denotes during
T5190 13696-13707 NN denotes development
T5191 13708-13710 IN denotes of
T5192 13711-13714 DT denotes the
T5194 13715-13720 NN denotes mouse
T5195 13721-13726 JJ denotes inner
T5193 13727-13730 NN denotes ear
T5196 13731-13734 VBZ denotes has
T5197 13735-13739 VBN denotes been
T5185 13740-13750 VBN denotes determined
T5198 13751-13753 TO denotes to
T5199 13754-13756 VB denotes be
T5200 13757-13766 JJ denotes embryonic
T5201 13767-13770 NN denotes day
T5202 13771-13772 -LRB- denotes (
T5203 13772-13774 NN denotes ED
T5204 13774-13775 -RRB- denotes )
T5205 13776-13778 CD denotes 13
T5206 13779-13780 -LRB- denotes [
T5207 13780-13782 CD denotes 14
T5208 13782-13783 -RRB- denotes ]
T5209 13783-13784 . denotes .
T5210 13784-14014 sentence denotes Morphologically detectable differences in the inner ears of Slc26a4+/+ and Slc26a4-/- mice become evident as early as ED 15, when Slc26a4-/- mice start to develop an enlarged endolymphatic space that persists into adulthood [14].
T5211 13785-13800 RB denotes Morphologically
T5212 13801-13811 JJ denotes detectable
T5213 13812-13823 NNS denotes differences
T5215 13824-13826 IN denotes in
T5216 13827-13830 DT denotes the
T5218 13831-13836 JJ denotes inner
T5217 13837-13841 NNS denotes ears
T5219 13842-13844 IN denotes of
T5220 13845-13852 NN denotes Slc26a4
T5222 13852-13853 SYM denotes +
T5223 13853-13854 HYPH denotes /
T5224 13854-13855 SYM denotes +
T5225 13856-13859 CC denotes and
T5226 13860-13867 NN denotes Slc26a4
T5227 13867-13868 SYM denotes -
T5228 13868-13869 HYPH denotes /
T5229 13869-13870 SYM denotes -
T5221 13871-13875 NNS denotes mice
T5214 13876-13882 VBP denotes become
T5230 13883-13890 JJ denotes evident
T5231 13891-13893 RB denotes as
T5232 13894-13899 RB denotes early
T5233 13900-13902 IN denotes as
T5234 13903-13905 NN denotes ED
T5235 13906-13908 CD denotes 15
T5236 13908-13910 , denotes ,
T5237 13910-13914 WRB denotes when
T5239 13915-13922 NN denotes Slc26a4
T5241 13922-13923 SYM denotes -
T5242 13923-13924 HYPH denotes /
T5243 13924-13925 SYM denotes -
T5240 13926-13930 NNS denotes mice
T5238 13931-13936 VBP denotes start
T5244 13937-13939 TO denotes to
T5245 13940-13947 VB denotes develop
T5246 13948-13950 DT denotes an
T5248 13951-13959 JJ denotes enlarged
T5249 13960-13973 JJ denotes endolymphatic
T5247 13974-13979 NN denotes space
T5250 13980-13984 WDT denotes that
T5251 13985-13993 VBZ denotes persists
T5252 13994-13998 IN denotes into
T5253 13999-14008 NN denotes adulthood
T5254 14009-14010 -LRB- denotes [
T5255 14010-14012 CD denotes 14
T5256 14012-14013 -RRB- denotes ]
T5257 14013-14014 . denotes .
T5258 14014-14159 sentence denotes Interestingly, sensory hair cells in the cochlea appear normal until postnatal day (PD) 7 but show clear evidence of degeneration by PD 15 [14].
T5259 14015-14028 RB denotes Interestingly
T5261 14028-14030 , denotes ,
T5262 14030-14037 JJ denotes sensory
T5264 14038-14042 NN denotes hair
T5263 14043-14048 NNS denotes cells
T5265 14049-14051 IN denotes in
T5266 14052-14055 DT denotes the
T5267 14056-14063 NN denotes cochlea
T5260 14064-14070 VBP denotes appear
T5268 14071-14077 JJ denotes normal
T5269 14078-14083 IN denotes until
T5270 14084-14093 JJ denotes postnatal
T5271 14094-14097 NN denotes day
T5272 14098-14099 -LRB- denotes (
T5273 14099-14101 NN denotes PD
T5274 14101-14102 -RRB- denotes )
T5275 14103-14104 CD denotes 7
T5276 14105-14108 CC denotes but
T5277 14109-14113 VBP denotes show
T5278 14114-14119 JJ denotes clear
T5279 14120-14128 NN denotes evidence
T5280 14129-14131 IN denotes of
T5281 14132-14144 NN denotes degeneration
T5282 14145-14147 IN denotes by
T5283 14148-14150 NN denotes PD
T5284 14151-14153 CD denotes 15
T5285 14154-14155 -LRB- denotes [
T5286 14155-14157 CD denotes 14
T5287 14157-14158 -RRB- denotes ]
T5288 14158-14159 . denotes .
T5289 14159-14315 sentence denotes These observations suggest that the cochlear environment supports the survival of sensory hair cells in spite of the enlargement of the endolymphatic duct.
T5290 14160-14165 DT denotes These
T5291 14166-14178 NNS denotes observations
T5292 14179-14186 VBP denotes suggest
T5293 14187-14191 IN denotes that
T5295 14192-14195 DT denotes the
T5297 14196-14204 JJ denotes cochlear
T5296 14205-14216 NN denotes environment
T5294 14217-14225 VBZ denotes supports
T5298 14226-14229 DT denotes the
T5299 14230-14238 NN denotes survival
T5300 14239-14241 IN denotes of
T5301 14242-14249 JJ denotes sensory
T5303 14250-14254 NN denotes hair
T5302 14255-14260 NNS denotes cells
T5304 14261-14263 IN denotes in
T5305 14264-14269 NN denotes spite
T5306 14270-14272 IN denotes of
T5307 14273-14276 DT denotes the
T5308 14277-14288 NN denotes enlargement
T5309 14289-14291 IN denotes of
T5310 14292-14295 DT denotes the
T5312 14296-14309 JJ denotes endolymphatic
T5311 14310-14314 NN denotes duct
T5313 14314-14315 . denotes .
T5314 14315-14482 sentence denotes A normal endolymphatic K+ concentration, which is critical for hair cell survival [20], is established at PD 3 [21] and may persist in Slc26a4-/- at least until PD 7.
T5315 14316-14317 DT denotes A
T5317 14318-14324 JJ denotes normal
T5318 14325-14338 JJ denotes endolymphatic
T5319 14339-14340 NN denotes K
T5320 14340-14341 SYM denotes +
T5316 14342-14355 NN denotes concentration
T5322 14355-14357 , denotes ,
T5323 14357-14362 WDT denotes which
T5324 14363-14365 VBZ denotes is
T5325 14366-14374 JJ denotes critical
T5326 14375-14378 IN denotes for
T5327 14379-14383 NN denotes hair
T5328 14384-14388 NN denotes cell
T5329 14389-14397 NN denotes survival
T5330 14398-14399 -LRB- denotes [
T5331 14399-14401 CD denotes 20
T5332 14401-14402 -RRB- denotes ]
T5333 14402-14404 , denotes ,
T5334 14404-14406 VBZ denotes is
T5321 14407-14418 VBN denotes established
T5335 14419-14421 IN denotes at
T5336 14422-14424 NN denotes PD
T5337 14425-14426 CD denotes 3
T5338 14427-14428 -LRB- denotes [
T5339 14428-14430 CD denotes 21
T5340 14430-14431 -RRB- denotes ]
T5341 14432-14435 CC denotes and
T5342 14436-14439 MD denotes may
T5343 14440-14447 VB denotes persist
T5344 14448-14450 IN denotes in
T5345 14451-14458 NN denotes Slc26a4
T5346 14458-14459 SYM denotes -
T5347 14459-14460 HYPH denotes /
T5348 14460-14461 SYM denotes -
T5349 14462-14464 RB denotes at
T5350 14465-14470 RBS denotes least
T5351 14471-14476 IN denotes until
T5352 14477-14479 NN denotes PD
T5353 14480-14481 CD denotes 7
T5354 14481-14482 . denotes .
T5355 14482-14601 sentence denotes The time period between PD 7 and 15 is the time when the endocochlear potential develops at the onset of hearing [22].
T5356 14483-14486 DT denotes The
T5358 14487-14491 NN denotes time
T5357 14492-14498 NN denotes period
T5360 14499-14506 IN denotes between
T5361 14507-14509 NN denotes PD
T5362 14510-14511 CD denotes 7
T5363 14512-14515 CC denotes and
T5364 14516-14518 CD denotes 15
T5359 14519-14521 VBZ denotes is
T5365 14522-14525 DT denotes the
T5366 14526-14530 NN denotes time
T5367 14531-14535 WRB denotes when
T5369 14536-14539 DT denotes the
T5371 14540-14552 JJ denotes endocochlear
T5370 14553-14562 NN denotes potential
T5368 14563-14571 VBZ denotes develops
T5372 14572-14574 IN denotes at
T5373 14575-14578 DT denotes the
T5374 14579-14584 NN denotes onset
T5375 14585-14587 IN denotes of
T5376 14588-14595 NN denotes hearing
T5377 14596-14597 -LRB- denotes [
T5378 14597-14599 CD denotes 22
T5379 14599-14600 -RRB- denotes ]
T5380 14600-14601 . denotes .
T5381 14601-14766 sentence denotes We hypothesized that a lack of a normal endocochlear potential or an alteration of the endolymphatic K+ concentration could account for deafness in Slc26a4-/- mice.
T5382 14602-14604 PRP denotes We
T5383 14605-14617 VBD denotes hypothesized
T5384 14618-14622 IN denotes that
T5386 14623-14624 DT denotes a
T5387 14625-14629 NN denotes lack
T5388 14630-14632 IN denotes of
T5389 14633-14634 DT denotes a
T5391 14635-14641 JJ denotes normal
T5392 14642-14654 JJ denotes endocochlear
T5390 14655-14664 NN denotes potential
T5393 14665-14667 CC denotes or
T5394 14668-14670 DT denotes an
T5395 14671-14681 NN denotes alteration
T5396 14682-14684 IN denotes of
T5397 14685-14688 DT denotes the
T5399 14689-14702 JJ denotes endolymphatic
T5400 14703-14704 NN denotes K
T5401 14704-14705 SYM denotes +
T5398 14706-14719 NN denotes concentration
T5402 14720-14725 MD denotes could
T5385 14726-14733 VB denotes account
T5403 14734-14737 IN denotes for
T5404 14738-14746 NN denotes deafness
T5405 14747-14749 IN denotes in
T5406 14750-14757 NN denotes Slc26a4
T5408 14757-14758 SYM denotes -
T5409 14758-14759 HYPH denotes /
T5410 14759-14760 SYM denotes -
T5407 14761-14765 NNS denotes mice
T5411 14765-14766 . denotes .
T5412 14766-14917 sentence denotes Measurements revealed that the endocochlear potential was absent but that the endolymphatic K+ concentration was normal in adult Slc26a4-/- (Fig. 2a).
T5413 14767-14779 NNS denotes Measurements
T5414 14780-14788 VBD denotes revealed
T5415 14789-14793 IN denotes that
T5417 14794-14797 DT denotes the
T5419 14798-14810 JJ denotes endocochlear
T5418 14811-14820 NN denotes potential
T5416 14821-14824 VBD denotes was
T5420 14825-14831 JJ denotes absent
T5421 14832-14835 CC denotes but
T5422 14836-14840 IN denotes that
T5424 14841-14844 DT denotes the
T5426 14845-14858 JJ denotes endolymphatic
T5427 14859-14860 NN denotes K
T5428 14860-14861 SYM denotes +
T5425 14862-14875 NN denotes concentration
T5423 14876-14879 VBD denotes was
T5429 14880-14886 JJ denotes normal
T5430 14887-14889 IN denotes in
T5431 14890-14895 JJ denotes adult
T5432 14896-14903 NN denotes Slc26a4
T5433 14903-14904 SYM denotes -
T5434 14904-14905 HYPH denotes /
T5435 14905-14906 SYM denotes -
T5436 14907-14908 -LRB- denotes (
T5437 14908-14912 NN denotes Fig.
T5438 14913-14915 CD denotes 2a
T5439 14915-14916 -RRB- denotes )
T5440 14916-14917 . denotes .
T5441 14917-15117 sentence denotes No significant differences between Slc26a4+/+ and Slc26a4-/- mice were found in perilymphatic (Fig. 2a) or plasma K+ concentrations (Slc26a4+/+, 4.9 ± 0.3 mM, n = 6; Slc26a4-/-, 5.1 ± 0.3 mM, n = 6).
T5442 14918-14920 DT denotes No
T5444 14921-14932 JJ denotes significant
T5443 14933-14944 NNS denotes differences
T5446 14945-14952 IN denotes between
T5447 14953-14960 NN denotes Slc26a4
T5449 14960-14961 SYM denotes +
T5450 14961-14962 HYPH denotes /
T5451 14962-14963 SYM denotes +
T5452 14964-14967 CC denotes and
T5453 14968-14975 NN denotes Slc26a4
T5454 14975-14976 SYM denotes -
T5455 14976-14977 HYPH denotes /
T5456 14977-14978 SYM denotes -
T5448 14979-14983 NNS denotes mice
T5457 14984-14988 VBD denotes were
T5445 14989-14994 VBN denotes found
T5458 14995-14997 IN denotes in
T5459 14998-15011 JJ denotes perilymphatic
T5461 15012-15013 -LRB- denotes (
T5462 15013-15017 NN denotes Fig.
T5463 15018-15020 CD denotes 2a
T5464 15020-15021 -RRB- denotes )
T5465 15022-15024 CC denotes or
T5466 15025-15031 NN denotes plasma
T5467 15032-15033 NN denotes K
T5468 15033-15034 SYM denotes +
T5460 15035-15049 NNS denotes concentrations
T5469 15050-15051 -LRB- denotes (
T5471 15051-15058 NN denotes Slc26a4
T5473 15058-15059 SYM denotes +
T5474 15059-15060 HYPH denotes /
T5475 15060-15061 SYM denotes +
T5476 15061-15063 , denotes ,
T5477 15063-15066 CD denotes 4.9
T5479 15067-15068 SYM denotes ±
T5478 15069-15072 CD denotes 0.3
T5480 15073-15075 NNS denotes mM
T5481 15075-15077 , denotes ,
T5482 15077-15078 NN denotes n
T5483 15079-15080 SYM denotes =
T5472 15081-15082 CD denotes 6
T5484 15082-15083 : denotes ;
T5485 15084-15091 NN denotes Slc26a4
T5486 15091-15092 SYM denotes -
T5487 15092-15093 HYPH denotes /
T5488 15093-15094 SYM denotes -
T5489 15094-15096 , denotes ,
T5490 15096-15099 CD denotes 5.1
T5492 15100-15101 SYM denotes ±
T5491 15102-15105 CD denotes 0.3
T5493 15106-15108 NNS denotes mM
T5494 15108-15110 , denotes ,
T5495 15110-15111 NN denotes n
T5496 15112-15113 SYM denotes =
T5470 15114-15115 CD denotes 6
T5497 15115-15116 -RRB- denotes )
T5498 15116-15117 . denotes .
T5499 15117-15309 sentence denotes These observations suggest that a primary event leading to deafness in Slc26a4-/- mice, and potentially in patients suffering from Pendred syndrome, is the loss of the endocochlear potential.
T5500 15118-15123 DT denotes These
T5501 15124-15136 NNS denotes observations
T5502 15137-15144 VBP denotes suggest
T5503 15145-15149 IN denotes that
T5505 15150-15151 DT denotes a
T5507 15152-15159 JJ denotes primary
T5506 15160-15165 NN denotes event
T5508 15166-15173 VBG denotes leading
T5509 15174-15176 IN denotes to
T5510 15177-15185 NN denotes deafness
T5511 15186-15188 IN denotes in
T5512 15189-15196 NN denotes Slc26a4
T5514 15196-15197 SYM denotes -
T5515 15197-15198 HYPH denotes /
T5516 15198-15199 SYM denotes -
T5513 15200-15204 NNS denotes mice
T5517 15204-15206 , denotes ,
T5518 15206-15209 CC denotes and
T5519 15210-15221 RB denotes potentially
T5520 15222-15224 IN denotes in
T5521 15225-15233 NNS denotes patients
T5522 15234-15243 VBG denotes suffering
T5523 15244-15248 IN denotes from
T5524 15249-15256 NNP denotes Pendred
T5525 15257-15265 NN denotes syndrome
T5526 15265-15267 , denotes ,
T5504 15267-15269 VBZ denotes is
T5527 15270-15273 DT denotes the
T5528 15274-15278 NN denotes loss
T5529 15279-15281 IN denotes of
T5530 15282-15285 DT denotes the
T5532 15286-15298 JJ denotes endocochlear
T5531 15299-15308 NN denotes potential
T5533 15308-15309 . denotes .
T5534 15309-15402 sentence denotes Degeneration of hair cells is probably a response to the loss of the endocochlear potential.
T5535 15310-15322 NN denotes Degeneration
T5537 15323-15325 IN denotes of
T5538 15326-15330 NN denotes hair
T5539 15331-15336 NNS denotes cells
T5536 15337-15339 VBZ denotes is
T5540 15340-15348 RB denotes probably
T5541 15349-15350 DT denotes a
T5542 15351-15359 NN denotes response
T5543 15360-15362 IN denotes to
T5544 15363-15366 DT denotes the
T5545 15367-15371 NN denotes loss
T5546 15372-15374 IN denotes of
T5547 15375-15378 DT denotes the
T5549 15379-15391 JJ denotes endocochlear
T5548 15392-15401 NN denotes potential
T5550 15401-15402 . denotes .
T5551 15402-15403 sentence denotes
T8159 15413-15422 JJ denotes Potential
T8160 15422-15424 , denotes ,
T8161 15424-15425 NN denotes K
T8163 15425-15426 SYM denotes +
T8162 15427-15441 NNS denotes concentrations
T8164 15442-15445 CC denotes and
T8165 15446-15458 NN denotes pigmentation
T8166 15459-15461 IN denotes of
T8167 15462-15467 NN denotes stria
T8168 15468-15478 NN denotes vascularis
T8169 15479-15481 IN denotes in
T8170 15482-15489 NN denotes Slc26a4
T8172 15489-15490 SYM denotes +
T8173 15490-15491 HYPH denotes /
T8174 15491-15492 SYM denotes +
T8175 15493-15496 CC denotes and
T8176 15497-15504 NN denotes Slc26a4
T8177 15504-15505 SYM denotes -
T8178 15505-15506 HYPH denotes /
T8179 15506-15507 SYM denotes -
T8171 15508-15512 NNS denotes mice
T8180 15512-15513 . denotes .
T8181 15513-15633 sentence denotes a: Endocochlear potential and K+ concentrations in endolymph and perilymph at the apex (A) and base (B) of the cochlea.
T8182 15514-15515 LS denotes a
T8184 15515-15517 : denotes :
T8185 15517-15529 JJ denotes Endocochlear
T8183 15530-15539 NN denotes potential
T8186 15540-15543 CC denotes and
T8187 15544-15545 NN denotes K
T8189 15545-15546 SYM denotes +
T8188 15547-15561 NNS denotes concentrations
T8190 15562-15564 IN denotes in
T8191 15565-15574 NN denotes endolymph
T8192 15575-15578 CC denotes and
T8193 15579-15588 NN denotes perilymph
T8194 15589-15591 IN denotes at
T8195 15592-15595 DT denotes the
T8196 15596-15600 NN denotes apex
T8197 15601-15602 -LRB- denotes (
T8198 15602-15603 NN denotes A
T8199 15603-15604 -RRB- denotes )
T8200 15605-15608 CC denotes and
T8201 15609-15613 NN denotes base
T8202 15614-15615 -LRB- denotes (
T8203 15615-15616 NN denotes B
T8204 15616-15617 -RRB- denotes )
T8205 15618-15620 IN denotes of
T8206 15621-15624 DT denotes the
T8207 15625-15632 NN denotes cochlea
T8208 15632-15633 . denotes .
T8209 15633-15692 sentence denotes Numbers adjacent to symbols denote number of measurements.
T8210 15634-15641 NNS denotes Numbers
T8212 15642-15650 JJ denotes adjacent
T8213 15651-15653 IN denotes to
T8214 15654-15661 NNS denotes symbols
T8211 15662-15668 VBP denotes denote
T8215 15669-15675 NN denotes number
T8216 15676-15678 IN denotes of
T8217 15679-15691 NNS denotes measurements
T8218 15691-15692 . denotes .
T8219 15692-15765 sentence denotes b-d: Pigmentation of stria vascularis in Slc26a4+/+ and Slc26a4-/- mice.
T8220 15693-15694 LS denotes b
T8222 15694-15695 SYM denotes -
T8223 15695-15696 LS denotes d
T8224 15696-15698 : denotes :
T8221 15698-15710 NN denotes Pigmentation
T8225 15711-15713 IN denotes of
T8226 15714-15719 NN denotes stria
T8227 15720-15730 NN denotes vascularis
T8228 15731-15733 IN denotes in
T8229 15734-15741 NN denotes Slc26a4
T8231 15741-15742 SYM denotes +
T8232 15742-15743 HYPH denotes /
T8233 15743-15744 SYM denotes +
T8234 15745-15748 CC denotes and
T8235 15749-15756 NN denotes Slc26a4
T8236 15756-15757 SYM denotes -
T8237 15757-15758 HYPH denotes /
T8238 15758-15759 SYM denotes -
T8230 15760-15764 NNS denotes mice
T8239 15764-15765 . denotes .
T8240 15765-15834 sentence denotes b: View of stria vascularis through the bony capsule of the cochlea.
T8241 15766-15767 LS denotes b
T8243 15767-15769 : denotes :
T8242 15769-15773 NN denotes View
T8244 15774-15776 IN denotes of
T8245 15777-15782 NN denotes stria
T8246 15783-15793 NN denotes vascularis
T8247 15794-15801 IN denotes through
T8248 15802-15805 DT denotes the
T8250 15806-15810 JJ denotes bony
T8249 15811-15818 NN denotes capsule
T8251 15819-15821 IN denotes of
T8252 15822-15825 DT denotes the
T8253 15826-15833 NN denotes cochlea
T8254 15833-15834 . denotes .
T8255 15834-15895 sentence denotes OW, oval window, RW, round window; arrows, stria vascularis.
T8256 15835-15837 NN denotes OW
T8257 15837-15839 , denotes ,
T8258 15839-15843 JJ denotes oval
T8259 15844-15850 NN denotes window
T8260 15850-15852 , denotes ,
T8261 15852-15854 NN denotes RW
T8262 15854-15856 , denotes ,
T8263 15856-15861 JJ denotes round
T8264 15862-15868 NN denotes window
T8265 15868-15869 : denotes ;
T8266 15870-15876 NNS denotes arrows
T8267 15876-15878 , denotes ,
T8268 15878-15883 NN denotes stria
T8269 15884-15894 NN denotes vascularis
T8270 15894-15895 . denotes .
T8271 15895-15965 sentence denotes c-d: Whole-mounts of stria vascularis isolated from age-matched mice.
T8272 15896-15897 LS denotes c
T8274 15897-15898 SYM denotes -
T8275 15898-15899 LS denotes d
T8276 15899-15901 : denotes :
T8277 15901-15906 JJ denotes Whole
T8278 15906-15907 HYPH denotes -
T8273 15907-15913 NNS denotes mounts
T8279 15914-15916 IN denotes of
T8280 15917-15922 NN denotes stria
T8281 15923-15933 NN denotes vascularis
T8282 15934-15942 VBN denotes isolated
T8283 15943-15947 IN denotes from
T8284 15948-15951 NN denotes age
T8286 15951-15952 HYPH denotes -
T8285 15952-15959 VBN denotes matched
T8287 15960-15964 NNS denotes mice
T8288 15964-15965 . denotes .
T8289 15965-16064 sentence denotes c: Laser-scanning images, bar = 10 μm, d: Quantification of pigmentation based on optical density.
T8290 15966-15967 LS denotes c
T8292 15967-15969 : denotes :
T8293 15969-15974 NN denotes Laser
T8295 15974-15975 HYPH denotes -
T8296 15975-15983 VBG denotes scanning
T8294 15984-15990 NNS denotes images
T8297 15990-15992 , denotes ,
T8298 15992-15995 NN denotes bar
T8299 15996-15997 SYM denotes =
T8300 15998-16000 CD denotes 10
T8291 16001-16003 NN denotes μm
T8302 16003-16005 , denotes ,
T8303 16005-16006 LS denotes d
T8304 16006-16008 : denotes :
T8301 16008-16022 NN denotes Quantification
T8305 16023-16025 IN denotes of
T8306 16026-16038 NN denotes pigmentation
T8307 16039-16044 VBN denotes based
T8308 16045-16047 IN denotes on
T8309 16048-16055 JJ denotes optical
T8310 16056-16063 NN denotes density
T8311 16063-16064 . denotes .
T5553 16065-16068 DT denotes The
T5552 16065-16168 sentence denotes The endocochlear potential is generated by stria vascularis in the lateral wall of the cochlea [17,23].
T5555 16069-16081 JJ denotes endocochlear
T5554 16082-16091 NN denotes potential
T5557 16092-16094 VBZ denotes is
T5556 16095-16104 VBN denotes generated
T5558 16105-16107 IN denotes by
T5559 16108-16113 NN denotes stria
T5560 16114-16124 NN denotes vascularis
T5561 16125-16127 IN denotes in
T5562 16128-16131 DT denotes the
T5564 16132-16139 JJ denotes lateral
T5563 16140-16144 NN denotes wall
T5565 16145-16147 IN denotes of
T5566 16148-16151 DT denotes the
T5567 16152-16159 NN denotes cochlea
T5568 16160-16161 -LRB- denotes [
T5570 16161-16163 CD denotes 17
T5571 16163-16164 , denotes ,
T5569 16164-16166 CD denotes 23
T5572 16166-16167 -RRB- denotes ]
T5573 16167-16168 . denotes .
T5574 16168-16382 sentence denotes The potential is generated across the basal cell barrier of stria vascularis by the K+ channel KCNJ10 located in intermediate cells [24], which are connected to basal cells by a high density of gap junctions [25].
T5575 16169-16172 DT denotes The
T5576 16173-16182 NN denotes potential
T5578 16183-16185 VBZ denotes is
T5577 16186-16195 VBN denotes generated
T5579 16196-16202 IN denotes across
T5580 16203-16206 DT denotes the
T5582 16207-16212 JJ denotes basal
T5583 16213-16217 NN denotes cell
T5581 16218-16225 NN denotes barrier
T5584 16226-16228 IN denotes of
T5585 16229-16234 NN denotes stria
T5586 16235-16245 NN denotes vascularis
T5587 16246-16248 IN denotes by
T5588 16249-16252 DT denotes the
T5590 16253-16254 NN denotes K
T5591 16254-16255 SYM denotes +
T5592 16256-16263 NN denotes channel
T5589 16264-16270 NN denotes KCNJ10
T5593 16271-16278 VBN denotes located
T5594 16279-16281 IN denotes in
T5595 16282-16294 JJ denotes intermediate
T5596 16295-16300 NNS denotes cells
T5597 16301-16302 -LRB- denotes [
T5598 16302-16304 CD denotes 24
T5599 16304-16305 -RRB- denotes ]
T5600 16305-16307 , denotes ,
T5601 16307-16312 WDT denotes which
T5603 16313-16316 VBP denotes are
T5602 16317-16326 VBN denotes connected
T5604 16327-16329 IN denotes to
T5605 16330-16335 JJ denotes basal
T5606 16336-16341 NNS denotes cells
T5607 16342-16344 IN denotes by
T5608 16345-16346 DT denotes a
T5610 16347-16351 JJ denotes high
T5609 16352-16359 NN denotes density
T5611 16360-16362 IN denotes of
T5612 16363-16366 NN denotes gap
T5613 16367-16376 NNS denotes junctions
T5614 16377-16378 -LRB- denotes [
T5615 16378-16380 CD denotes 25
T5616 16380-16381 -RRB- denotes ]
T5617 16381-16382 . denotes .
T5618 16382-16588 sentence denotes Marginal cells of stria vascularis, which form the barrier toward endolymph, transport K+ from the intrastrial space into endolymph and keep the K+ concentration low adjacent to the KCNJ10 K+ channel [26].
T5619 16383-16391 JJ denotes Marginal
T5620 16392-16397 NNS denotes cells
T5622 16398-16400 IN denotes of
T5623 16401-16406 NN denotes stria
T5624 16407-16417 NN denotes vascularis
T5625 16417-16419 , denotes ,
T5626 16419-16424 WDT denotes which
T5627 16425-16429 VBP denotes form
T5628 16430-16433 DT denotes the
T5629 16434-16441 NN denotes barrier
T5630 16442-16448 IN denotes toward
T5631 16449-16458 NN denotes endolymph
T5632 16458-16460 , denotes ,
T5621 16460-16469 VBP denotes transport
T5633 16470-16471 NN denotes K
T5634 16471-16472 SYM denotes +
T5635 16473-16477 IN denotes from
T5636 16478-16481 DT denotes the
T5638 16482-16493 JJ denotes intrastrial
T5637 16494-16499 NN denotes space
T5639 16500-16504 IN denotes into
T5640 16505-16514 NN denotes endolymph
T5641 16515-16518 CC denotes and
T5642 16519-16523 VB denotes keep
T5643 16524-16527 DT denotes the
T5645 16528-16529 NN denotes K
T5646 16529-16530 SYM denotes +
T5644 16531-16544 NN denotes concentration
T5647 16545-16548 JJ denotes low
T5648 16549-16557 JJ denotes adjacent
T5649 16558-16560 IN denotes to
T5650 16561-16564 DT denotes the
T5652 16565-16571 NN denotes KCNJ10
T5653 16572-16573 NN denotes K
T5654 16573-16574 SYM denotes +
T5651 16575-16582 NN denotes channel
T5655 16583-16584 -LRB- denotes [
T5656 16584-16586 CD denotes 26
T5657 16586-16587 -RRB- denotes ]
T5658 16587-16588 . denotes .
T5659 16588-16854 sentence denotes To determine the cause of the loss of the endocochlear potential in Slc26a4-/- mice, we first determined whether intermediate cells are present in stria vascularis, since a loss of intermediate cells is known to lead to a loss of the endocochlear potential [27,28].
T5660 16589-16591 TO denotes To
T5661 16592-16601 VB denotes determine
T5663 16602-16605 DT denotes the
T5664 16606-16611 NN denotes cause
T5665 16612-16614 IN denotes of
T5666 16615-16618 DT denotes the
T5667 16619-16623 NN denotes loss
T5668 16624-16626 IN denotes of
T5669 16627-16630 DT denotes the
T5671 16631-16643 JJ denotes endocochlear
T5670 16644-16653 NN denotes potential
T5672 16654-16656 IN denotes in
T5673 16657-16664 NN denotes Slc26a4
T5675 16664-16665 SYM denotes -
T5676 16665-16666 HYPH denotes /
T5677 16666-16667 SYM denotes -
T5674 16668-16672 NNS denotes mice
T5678 16672-16674 , denotes ,
T5679 16674-16676 PRP denotes we
T5680 16677-16682 RB denotes first
T5662 16683-16693 VBD denotes determined
T5681 16694-16701 IN denotes whether
T5683 16702-16714 JJ denotes intermediate
T5684 16715-16720 NNS denotes cells
T5682 16721-16724 VBP denotes are
T5685 16725-16732 JJ denotes present
T5686 16733-16735 IN denotes in
T5687 16736-16741 NN denotes stria
T5688 16742-16752 NN denotes vascularis
T5689 16752-16754 , denotes ,
T5690 16754-16759 IN denotes since
T5692 16760-16761 DT denotes a
T5693 16762-16766 NN denotes loss
T5694 16767-16769 IN denotes of
T5695 16770-16782 JJ denotes intermediate
T5696 16783-16788 NNS denotes cells
T5697 16789-16791 VBZ denotes is
T5691 16792-16797 VBN denotes known
T5698 16798-16800 TO denotes to
T5699 16801-16805 VB denotes lead
T5700 16806-16808 IN denotes to
T5701 16809-16810 DT denotes a
T5702 16811-16815 NN denotes loss
T5703 16816-16818 IN denotes of
T5704 16819-16822 DT denotes the
T5706 16823-16835 JJ denotes endocochlear
T5705 16836-16845 NN denotes potential
T5707 16846-16847 -LRB- denotes [
T5709 16847-16849 CD denotes 27
T5710 16849-16850 , denotes ,
T5708 16850-16852 CD denotes 28
T5711 16852-16853 -RRB- denotes ]
T5712 16853-16854 . denotes .
T5713 16854-16932 sentence denotes Intermediate cells of stria vascularis were visualized by their pigmentation.
T5714 16855-16867 JJ denotes Intermediate
T5715 16868-16873 NNS denotes cells
T5717 16874-16876 IN denotes of
T5718 16877-16882 NN denotes stria
T5719 16883-16893 NN denotes vascularis
T5720 16894-16898 VBD denotes were
T5716 16899-16909 VBN denotes visualized
T5721 16910-16912 IN denotes by
T5722 16913-16918 PRP$ denotes their
T5723 16919-16931 NN denotes pigmentation
T5724 16931-16932 . denotes .
T5725 16932-17059 sentence denotes Pigmentation was present in stria vascularis of Slc26a4-/- mice (Fig. 2b), which suggests that intermediate cells are present.
T5726 16933-16945 NN denotes Pigmentation
T5727 16946-16949 VBD denotes was
T5728 16950-16957 JJ denotes present
T5729 16958-16960 IN denotes in
T5730 16961-16966 NN denotes stria
T5731 16967-16977 NN denotes vascularis
T5732 16978-16980 IN denotes of
T5733 16981-16988 NN denotes Slc26a4
T5735 16988-16989 SYM denotes -
T5736 16989-16990 HYPH denotes /
T5737 16990-16991 SYM denotes -
T5734 16992-16996 NNS denotes mice
T5738 16997-16998 -LRB- denotes (
T5739 16998-17002 NN denotes Fig.
T5740 17003-17005 CD denotes 2b
T5741 17005-17006 -RRB- denotes )
T5742 17006-17008 , denotes ,
T5743 17008-17013 WDT denotes which
T5744 17014-17022 VBZ denotes suggests
T5745 17023-17027 IN denotes that
T5747 17028-17040 JJ denotes intermediate
T5748 17041-17046 NNS denotes cells
T5746 17047-17050 VBP denotes are
T5749 17051-17058 JJ denotes present
T5750 17058-17059 . denotes .
T5751 17059-17164 sentence denotes Interestingly, pigmentation of stria vascularis was much stronger in Slc26a4-/- than in Slc26a4+/+ mice.
T5752 17060-17073 RB denotes Interestingly
T5754 17073-17075 , denotes ,
T5755 17075-17087 NN denotes pigmentation
T5756 17088-17090 IN denotes of
T5757 17091-17096 NN denotes stria
T5758 17097-17107 NN denotes vascularis
T5753 17108-17111 VBD denotes was
T5759 17112-17116 RB denotes much
T5760 17117-17125 JJR denotes stronger
T5761 17126-17128 IN denotes in
T5762 17129-17136 NN denotes Slc26a4
T5763 17136-17137 SYM denotes -
T5764 17137-17138 HYPH denotes /
T5765 17138-17139 SYM denotes -
T5766 17140-17144 IN denotes than
T5767 17145-17147 IN denotes in
T5768 17148-17155 NN denotes Slc26a4
T5770 17155-17156 SYM denotes +
T5771 17156-17157 HYPH denotes /
T5772 17157-17158 SYM denotes +
T5769 17159-17163 NNS denotes mice
T5773 17163-17164 . denotes .
T5774 17164-17528 sentence denotes To determine in greater detail the cause of the loss of the endocochlear potential in Slc26a4-/- mice, we isolated total RNA from stria vascularis and spiral ganglia of young (1–4 month) Slc26a4+/+ and both young and old (~12 month) Slc26a4-/- mice, assessed amounts of isolated tissues by quantification of 18S rRNA, and quantified the expression of KCNJ10 mRNA.
T5775 17165-17167 TO denotes To
T5776 17168-17177 VB denotes determine
T5778 17178-17180 IN denotes in
T5779 17181-17188 JJR denotes greater
T5780 17189-17195 NN denotes detail
T5781 17196-17199 DT denotes the
T5782 17200-17205 NN denotes cause
T5783 17206-17208 IN denotes of
T5784 17209-17212 DT denotes the
T5785 17213-17217 NN denotes loss
T5786 17218-17220 IN denotes of
T5787 17221-17224 DT denotes the
T5789 17225-17237 JJ denotes endocochlear
T5788 17238-17247 NN denotes potential
T5790 17248-17250 IN denotes in
T5791 17251-17258 NN denotes Slc26a4
T5793 17258-17259 SYM denotes -
T5794 17259-17260 HYPH denotes /
T5795 17260-17261 SYM denotes -
T5792 17262-17266 NNS denotes mice
T5796 17266-17268 , denotes ,
T5797 17268-17270 PRP denotes we
T5777 17271-17279 VBD denotes isolated
T5798 17280-17285 JJ denotes total
T5799 17286-17289 NN denotes RNA
T5800 17290-17294 IN denotes from
T5801 17295-17300 NN denotes stria
T5802 17301-17311 NN denotes vascularis
T5803 17312-17315 CC denotes and
T5804 17316-17322 JJ denotes spiral
T5805 17323-17330 NNS denotes ganglia
T5806 17331-17333 IN denotes of
T5807 17334-17339 JJ denotes young
T5809 17340-17341 -LRB- denotes (
T5811 17341-17342 CD denotes 1
T5813 17342-17343 SYM denotes –
T5812 17343-17344 CD denotes 4
T5810 17345-17350 NN denotes month
T5814 17350-17351 -RRB- denotes )
T5808 17352-17359 NN denotes Slc26a4
T5815 17359-17360 SYM denotes +
T5816 17360-17361 HYPH denotes /
T5817 17361-17362 SYM denotes +
T5818 17363-17366 CC denotes and
T5819 17367-17371 CC denotes both
T5821 17372-17377 JJ denotes young
T5822 17378-17381 CC denotes and
T5823 17382-17385 JJ denotes old
T5824 17386-17387 -LRB- denotes (
T5826 17387-17388 SYM denotes ~
T5827 17388-17390 CD denotes 12
T5825 17391-17396 NN denotes month
T5828 17396-17397 -RRB- denotes )
T5820 17398-17405 NN denotes Slc26a4
T5830 17405-17406 SYM denotes -
T5831 17406-17407 HYPH denotes /
T5832 17407-17408 SYM denotes -
T5829 17409-17413 NNS denotes mice
T5833 17413-17415 , denotes ,
T5834 17415-17423 VBN denotes assessed
T5835 17424-17431 NNS denotes amounts
T5836 17432-17434 IN denotes of
T5837 17435-17443 VBN denotes isolated
T5838 17444-17451 NNS denotes tissues
T5839 17452-17454 IN denotes by
T5840 17455-17469 NN denotes quantification
T5841 17470-17472 IN denotes of
T5842 17473-17476 NN denotes 18S
T5843 17477-17481 NN denotes rRNA
T5844 17481-17483 , denotes ,
T5845 17483-17486 CC denotes and
T5846 17487-17497 VBD denotes quantified
T5847 17498-17501 DT denotes the
T5848 17502-17512 NN denotes expression
T5849 17513-17515 IN denotes of
T5850 17516-17522 NN denotes KCNJ10
T5851 17523-17527 NN denotes mRNA
T5852 17527-17528 . denotes .
T5853 17528-17737 sentence denotes Quantities of stria vascularis isolated from these different mice were similar, since no significant differences were found in the numbers of 18S rRNA molecules (log(rRNA) = 9.46 ± 0.08, n = 17, pooled data).
T5854 17529-17539 NNS denotes Quantities
T5856 17540-17542 IN denotes of
T5857 17543-17548 NN denotes stria
T5858 17549-17559 NN denotes vascularis
T5859 17560-17568 VBN denotes isolated
T5860 17569-17573 IN denotes from
T5861 17574-17579 DT denotes these
T5863 17580-17589 JJ denotes different
T5862 17590-17594 NNS denotes mice
T5855 17595-17599 VBD denotes were
T5864 17600-17607 JJ denotes similar
T5865 17607-17609 , denotes ,
T5866 17609-17614 IN denotes since
T5868 17615-17617 DT denotes no
T5870 17618-17629 JJ denotes significant
T5869 17630-17641 NNS denotes differences
T5871 17642-17646 VBD denotes were
T5867 17647-17652 VBN denotes found
T5872 17653-17655 IN denotes in
T5873 17656-17659 DT denotes the
T5874 17660-17667 NNS denotes numbers
T5875 17668-17670 IN denotes of
T5876 17671-17674 NN denotes 18S
T5878 17675-17679 NN denotes rRNA
T5877 17680-17689 NNS denotes molecules
T5879 17690-17691 -LRB- denotes (
T5881 17691-17694 NN denotes log
T5883 17694-17695 -LRB- denotes (
T5882 17695-17699 NN denotes rRNA
T5885 17699-17700 -RRB- denotes )
T5886 17701-17702 SYM denotes =
T5887 17703-17707 CD denotes 9.46
T5888 17708-17709 SYM denotes ±
T5884 17710-17714 CD denotes 0.08
T5889 17714-17716 , denotes ,
T5890 17716-17717 NN denotes n
T5892 17718-17719 SYM denotes =
T5891 17720-17722 CD denotes 17
T5893 17722-17724 , denotes ,
T5894 17724-17730 JJ denotes pooled
T5880 17731-17735 NNS denotes data
T5895 17735-17736 -RRB- denotes )
T5896 17736-17737 . denotes .
T5897 17737-18011 sentence denotes In contrast, quantities of spiral ganglia isolated from young and old Slc26a4-/- mice (log(rRNA) = 9.04 ± 0.18, n = 4 and 9.29 ± 0.15, n = 6) were significantly smaller than in Slc26a4+/+ mice (log(rRNA) = 9.48 ± 0.19, n = 7), consistent with morphometric data (see below).
T5898 17738-17740 IN denotes In
T5900 17741-17749 NN denotes contrast
T5901 17749-17751 , denotes ,
T5902 17751-17761 NNS denotes quantities
T5903 17762-17764 IN denotes of
T5904 17765-17771 JJ denotes spiral
T5905 17772-17779 NNS denotes ganglia
T5906 17780-17788 VBN denotes isolated
T5907 17789-17793 IN denotes from
T5908 17794-17799 JJ denotes young
T5910 17800-17803 CC denotes and
T5911 17804-17807 JJ denotes old
T5912 17808-17815 NN denotes Slc26a4
T5913 17815-17816 SYM denotes -
T5914 17816-17817 HYPH denotes /
T5915 17817-17818 SYM denotes -
T5909 17819-17823 NNS denotes mice
T5916 17824-17825 -LRB- denotes (
T5918 17825-17828 NN denotes log
T5920 17828-17829 -LRB- denotes (
T5919 17829-17833 NN denotes rRNA
T5921 17833-17834 -RRB- denotes )
T5922 17835-17836 SYM denotes =
T5923 17837-17841 CD denotes 9.04
T5924 17842-17843 SYM denotes ±
T5917 17844-17848 CD denotes 0.18
T5925 17848-17850 , denotes ,
T5927 17850-17851 NN denotes n
T5928 17852-17853 SYM denotes =
T5926 17854-17855 CD denotes 4
T5929 17856-17859 CC denotes and
T5930 17860-17864 CD denotes 9.29
T5932 17865-17866 SYM denotes ±
T5931 17867-17871 CD denotes 0.15
T5933 17871-17873 , denotes ,
T5935 17873-17874 NN denotes n
T5936 17875-17876 SYM denotes =
T5934 17877-17878 CD denotes 6
T5937 17878-17879 -RRB- denotes )
T5899 17880-17884 VBD denotes were
T5938 17885-17898 RB denotes significantly
T5939 17899-17906 JJR denotes smaller
T5940 17907-17911 IN denotes than
T5941 17912-17914 IN denotes in
T5942 17915-17922 NN denotes Slc26a4
T5944 17922-17923 SYM denotes +
T5945 17923-17924 HYPH denotes /
T5946 17924-17925 SYM denotes +
T5943 17926-17930 NNS denotes mice
T5947 17931-17932 -LRB- denotes (
T5949 17932-17935 NN denotes log
T5951 17935-17936 -LRB- denotes (
T5950 17936-17940 NN denotes rRNA
T5953 17940-17941 -RRB- denotes )
T5954 17942-17943 SYM denotes =
T5955 17944-17948 CD denotes 9.48
T5956 17949-17950 SYM denotes ±
T5952 17951-17955 CD denotes 0.19
T5957 17955-17957 , denotes ,
T5958 17957-17958 NN denotes n
T5959 17959-17960 SYM denotes =
T5948 17961-17962 CD denotes 7
T5960 17962-17963 -RRB- denotes )
T5961 17963-17965 , denotes ,
T5962 17965-17975 JJ denotes consistent
T5963 17976-17980 IN denotes with
T5964 17981-17993 JJ denotes morphometric
T5965 17994-17998 NNS denotes data
T5966 17999-18000 -LRB- denotes (
T5967 18000-18003 VB denotes see
T5968 18004-18009 RB denotes below
T5969 18009-18010 -RRB- denotes )
T5970 18010-18011 . denotes .
T5971 18011-18171 sentence denotes Expression of KCNJ10 mRNA was normal in stria vascularis and spiral ganglia of young Slc26a4-/- mice but significantly reduced in old Slc26a4-/- mice (Fig. 3).
T5972 18012-18022 NN denotes Expression
T5974 18023-18025 IN denotes of
T5975 18026-18032 NN denotes KCNJ10
T5976 18033-18037 NN denotes mRNA
T5973 18038-18041 VBD denotes was
T5977 18042-18048 JJ denotes normal
T5978 18049-18051 IN denotes in
T5979 18052-18057 NN denotes stria
T5980 18058-18068 NN denotes vascularis
T5981 18069-18072 CC denotes and
T5982 18073-18079 JJ denotes spiral
T5983 18080-18087 NNS denotes ganglia
T5984 18088-18090 IN denotes of
T5985 18091-18096 JJ denotes young
T5987 18097-18104 NN denotes Slc26a4
T5988 18104-18105 SYM denotes -
T5989 18105-18106 HYPH denotes /
T5990 18106-18107 SYM denotes -
T5986 18108-18112 NNS denotes mice
T5991 18113-18116 CC denotes but
T5992 18117-18130 RB denotes significantly
T5993 18131-18138 VBN denotes reduced
T5994 18139-18141 IN denotes in
T5995 18142-18145 JJ denotes old
T5997 18146-18153 NN denotes Slc26a4
T5998 18153-18154 SYM denotes -
T5999 18154-18155 HYPH denotes /
T6000 18155-18156 SYM denotes -
T5996 18157-18161 NNS denotes mice
T6001 18162-18163 -LRB- denotes (
T6002 18163-18167 NN denotes Fig.
T6003 18168-18169 CD denotes 3
T6004 18169-18170 -RRB- denotes )
T6005 18170-18171 . denotes .
T6006 18171-18546 sentence denotes Quantifications of KCNQ1 and KCNQ4 mRNA were used to assess possible cross-contamination between the stria vascularis and spiral ganglia preparations based on the assumptions that (1) KCNQ1 is expressed in cells of the stria vascularis but not the spiral ganglia preparation and (2) KCNQ4 is expressed in cells of the spiral ganglia but not the stria vascularis preparation.
T6007 18172-18187 NNS denotes Quantifications
T6009 18188-18190 IN denotes of
T6010 18191-18196 NN denotes KCNQ1
T6012 18197-18200 CC denotes and
T6013 18201-18206 NN denotes KCNQ4
T6011 18207-18211 NN denotes mRNA
T6014 18212-18216 VBD denotes were
T6008 18217-18221 VBN denotes used
T6015 18222-18224 TO denotes to
T6016 18225-18231 VB denotes assess
T6017 18232-18240 JJ denotes possible
T6018 18241-18260 NN denotes cross-contamination
T6019 18261-18268 IN denotes between
T6020 18269-18272 DT denotes the
T6022 18273-18278 NN denotes stria
T6023 18279-18289 NN denotes vascularis
T6024 18290-18293 CC denotes and
T6025 18294-18300 JJ denotes spiral
T6026 18301-18308 NNS denotes ganglia
T6021 18309-18321 NNS denotes preparations
T6027 18322-18327 VBN denotes based
T6028 18328-18330 IN denotes on
T6029 18331-18334 DT denotes the
T6030 18335-18346 NNS denotes assumptions
T6031 18347-18351 IN denotes that
T6033 18352-18353 -LRB- denotes (
T6034 18353-18354 LS denotes 1
T6035 18354-18355 -RRB- denotes )
T6036 18356-18361 NN denotes KCNQ1
T6037 18362-18364 VBZ denotes is
T6032 18365-18374 VBN denotes expressed
T6038 18375-18377 IN denotes in
T6039 18378-18383 NNS denotes cells
T6040 18384-18386 IN denotes of
T6041 18387-18390 DT denotes the
T6043 18391-18396 NN denotes stria
T6042 18397-18407 NN denotes vascularis
T6044 18408-18411 CC denotes but
T6045 18412-18415 RB denotes not
T6046 18416-18419 DT denotes the
T6048 18420-18426 JJ denotes spiral
T6049 18427-18434 NNS denotes ganglia
T6047 18435-18446 NN denotes preparation
T6050 18447-18450 CC denotes and
T6051 18451-18452 -LRB- denotes (
T6052 18452-18453 LS denotes 2
T6054 18453-18454 -RRB- denotes )
T6055 18455-18460 NN denotes KCNQ4
T6056 18461-18463 VBZ denotes is
T6053 18464-18473 VBN denotes expressed
T6057 18474-18476 IN denotes in
T6058 18477-18482 NNS denotes cells
T6059 18483-18485 IN denotes of
T6060 18486-18489 DT denotes the
T6062 18490-18496 JJ denotes spiral
T6061 18497-18504 NNS denotes ganglia
T6063 18505-18508 CC denotes but
T6064 18509-18512 RB denotes not
T6065 18513-18516 DT denotes the
T6067 18517-18522 NN denotes stria
T6068 18523-18533 NN denotes vascularis
T6066 18534-18545 NN denotes preparation
T6069 18545-18546 . denotes .
T6070 18546-18747 sentence denotes The number of KCNQ1 mRNA molecules in stria vascularis was 24-fold greater than in spiral ganglia, and the number of KCNQ4 mRNA molecules in spiral ganglia was 5-fold greater than in stria vascularis.
T6071 18547-18550 DT denotes The
T6072 18551-18557 NN denotes number
T6074 18558-18560 IN denotes of
T6075 18561-18566 NN denotes KCNQ1
T6077 18567-18571 NN denotes mRNA
T6076 18572-18581 NNS denotes molecules
T6078 18582-18584 IN denotes in
T6079 18585-18590 NN denotes stria
T6080 18591-18601 NN denotes vascularis
T6073 18602-18605 VBD denotes was
T6081 18606-18608 CD denotes 24
T6083 18608-18609 HYPH denotes -
T6084 18609-18613 RB denotes fold
T6082 18614-18621 JJR denotes greater
T6085 18622-18626 IN denotes than
T6086 18627-18629 IN denotes in
T6087 18630-18636 JJ denotes spiral
T6088 18637-18644 NNS denotes ganglia
T6089 18644-18646 , denotes ,
T6090 18646-18649 CC denotes and
T6091 18650-18653 DT denotes the
T6092 18654-18660 NN denotes number
T6094 18661-18663 IN denotes of
T6095 18664-18669 NN denotes KCNQ4
T6097 18670-18674 NN denotes mRNA
T6096 18675-18684 NNS denotes molecules
T6098 18685-18687 IN denotes in
T6099 18688-18694 JJ denotes spiral
T6100 18695-18702 NNS denotes ganglia
T6093 18703-18706 VBD denotes was
T6101 18707-18708 CD denotes 5
T6103 18708-18709 HYPH denotes -
T6104 18709-18713 RB denotes fold
T6102 18714-18721 JJR denotes greater
T6105 18722-18726 IN denotes than
T6106 18727-18729 IN denotes in
T6107 18730-18735 NN denotes stria
T6108 18736-18746 NN denotes vascularis
T6109 18746-18747 . denotes .
T6110 18747-18942 sentence denotes These observations validate our measurements of KCNJ10 and KCNQ1 by demonstrating that the microdissected preparations of stria vascularis and spiral ganglia were 98% and 78% pure, respectively.
T6111 18748-18753 DT denotes These
T6112 18754-18766 NNS denotes observations
T6113 18767-18775 VBP denotes validate
T6114 18776-18779 PRP$ denotes our
T6115 18780-18792 NNS denotes measurements
T6116 18793-18795 IN denotes of
T6117 18796-18802 NN denotes KCNJ10
T6118 18803-18806 CC denotes and
T6119 18807-18812 NN denotes KCNQ1
T6120 18813-18815 IN denotes by
T6121 18816-18829 VBG denotes demonstrating
T6122 18830-18834 IN denotes that
T6124 18835-18838 DT denotes the
T6126 18839-18853 JJ denotes microdissected
T6125 18854-18866 NNS denotes preparations
T6127 18867-18869 IN denotes of
T6128 18870-18875 NN denotes stria
T6129 18876-18886 NN denotes vascularis
T6130 18887-18890 CC denotes and
T6131 18891-18897 JJ denotes spiral
T6132 18898-18905 NNS denotes ganglia
T6123 18906-18910 VBD denotes were
T6133 18911-18913 CD denotes 98
T6134 18913-18914 NN denotes %
T6136 18915-18918 CC denotes and
T6137 18919-18921 CD denotes 78
T6138 18921-18922 NN denotes %
T6135 18923-18927 JJ denotes pure
T6139 18927-18929 , denotes ,
T6140 18929-18941 RB denotes respectively
T6141 18941-18942 . denotes .
T6142 18942-18943 sentence denotes
T8435 18953-18967 NN denotes Quantification
T8436 18968-18970 IN denotes of
T8437 18971-18977 NN denotes KCNJ10
T8439 18978-18981 CC denotes and
T8440 18982-18987 NN denotes KCNQ1
T8441 18988-18992 NN denotes mRNA
T8438 18993-19003 NN denotes expression
T8442 19004-19006 IN denotes in
T8443 19007-19012 NN denotes stria
T8444 19013-19023 NN denotes vascularis
T8445 19024-19027 CC denotes and
T8446 19028-19034 JJ denotes spiral
T8447 19035-19042 NNS denotes ganglia
T8448 19043-19045 IN denotes of
T8449 19046-19053 NN denotes Slc26a4
T8451 19053-19054 SYM denotes +
T8452 19054-19055 HYPH denotes /
T8453 19055-19056 SYM denotes +
T8454 19057-19060 CC denotes and
T8455 19061-19068 NN denotes Slc26a4
T8456 19068-19069 SYM denotes -
T8457 19069-19070 HYPH denotes /
T8458 19070-19071 SYM denotes -
T8450 19072-19076 NNS denotes mice
T8459 19076-19077 . denotes .
T8460 19077-19172 sentence denotes a: Electropherogram of total RNA isolated from stria vascularis microdissected from one mouse.
T8461 19078-19079 LS denotes a
T8463 19079-19081 : denotes :
T8462 19081-19097 NN denotes Electropherogram
T8464 19098-19100 IN denotes of
T8465 19101-19106 JJ denotes total
T8466 19107-19110 NN denotes RNA
T8467 19111-19119 VBN denotes isolated
T8468 19120-19124 IN denotes from
T8469 19125-19130 NN denotes stria
T8470 19131-19141 NN denotes vascularis
T8471 19142-19156 VBN denotes microdissected
T8472 19157-19161 IN denotes from
T8473 19162-19165 CD denotes one
T8474 19166-19171 NN denotes mouse
T8475 19171-19172 . denotes .
T8476 19172-19317 sentence denotes The amount of total RNA was obtained from the total integral (shaded) and the amount of 18S rRNA was obtained from the integral of the 18S peak.
T8477 19173-19176 DT denotes The
T8478 19177-19183 NN denotes amount
T8480 19184-19186 IN denotes of
T8481 19187-19192 JJ denotes total
T8482 19193-19196 NN denotes RNA
T8483 19197-19200 VBD denotes was
T8479 19201-19209 VBN denotes obtained
T8484 19210-19214 IN denotes from
T8485 19215-19218 DT denotes the
T8487 19219-19224 JJ denotes total
T8486 19225-19233 NN denotes integral
T8488 19234-19235 -LRB- denotes (
T8489 19235-19241 JJ denotes shaded
T8490 19241-19242 -RRB- denotes )
T8491 19243-19246 CC denotes and
T8492 19247-19250 DT denotes the
T8493 19251-19257 NN denotes amount
T8495 19258-19260 IN denotes of
T8496 19261-19264 NN denotes 18S
T8497 19265-19269 NN denotes rRNA
T8498 19270-19273 VBD denotes was
T8494 19274-19282 VBN denotes obtained
T8499 19283-19287 IN denotes from
T8500 19288-19291 DT denotes the
T8501 19292-19300 NN denotes integral
T8502 19301-19303 IN denotes of
T8503 19304-19307 DT denotes the
T8505 19308-19311 NN denotes 18S
T8504 19312-19316 NN denotes peak
T8506 19316-19317 . denotes .
T8507 19317-19396 sentence denotes Sharp peaks representing 18S and 28S rRNA demonstrate the high quality of RNA.
T8508 19318-19323 JJ denotes Sharp
T8509 19324-19329 NNS denotes peaks
T8511 19330-19342 VBG denotes representing
T8512 19343-19346 NN denotes 18S
T8514 19347-19350 CC denotes and
T8515 19351-19354 NN denotes 28S
T8513 19355-19359 NN denotes rRNA
T8510 19360-19371 VBP denotes demonstrate
T8516 19372-19375 DT denotes the
T8518 19376-19380 JJ denotes high
T8517 19381-19388 NN denotes quality
T8519 19389-19391 IN denotes of
T8520 19392-19395 NN denotes RNA
T8521 19395-19396 . denotes .
T8522 19396-19543 sentence denotes Insert: Genotype of Slc26a4-/- mice was verified by the observation of one or few very large rhomboedric otoconia in the utricular macula (arrow).
T8523 19397-19403 NN denotes Insert
T8525 19403-19405 : denotes :
T8526 19405-19413 NN denotes Genotype
T8527 19414-19416 IN denotes of
T8528 19417-19424 NN denotes Slc26a4
T8530 19424-19425 SYM denotes -
T8531 19425-19426 HYPH denotes /
T8532 19426-19427 SYM denotes -
T8529 19428-19432 NNS denotes mice
T8533 19433-19436 VBD denotes was
T8524 19437-19445 VBN denotes verified
T8534 19446-19448 IN denotes by
T8535 19449-19452 DT denotes the
T8536 19453-19464 NN denotes observation
T8537 19465-19467 IN denotes of
T8538 19468-19471 CD denotes one
T8540 19472-19474 CC denotes or
T8541 19475-19478 JJ denotes few
T8542 19479-19483 RB denotes very
T8543 19484-19489 JJ denotes large
T8544 19490-19501 JJ denotes rhomboedric
T8539 19502-19510 NNS denotes otoconia
T8545 19511-19513 IN denotes in
T8546 19514-19517 DT denotes the
T8548 19518-19527 JJ denotes utricular
T8547 19528-19534 NN denotes macula
T8549 19535-19536 -LRB- denotes (
T8550 19536-19541 NN denotes arrow
T8551 19541-19542 -RRB- denotes )
T8552 19542-19543 . denotes .
T8553 19543-19586 sentence denotes A, crista ampullaris; U, utricular macula.
T8554 19544-19545 NN denotes A
T8555 19545-19547 , denotes ,
T8556 19547-19553 NN denotes crista
T8557 19554-19564 NN denotes ampullaris
T8558 19564-19565 : denotes ;
T8559 19566-19567 NN denotes U
T8560 19567-19569 , denotes ,
T8561 19569-19578 JJ denotes utricular
T8562 19579-19585 NN denotes macula
T8563 19585-19586 . denotes .
T8564 19586-19605 sentence denotes Scale bar: 100 μm.
T8565 19587-19592 NN denotes Scale
T8566 19593-19596 NN denotes bar
T8568 19596-19598 : denotes :
T8569 19598-19601 CD denotes 100
T8567 19602-19604 NNS denotes μm
T8570 19604-19605 . denotes .
T8571 19605-19698 sentence denotes b: Example of real-time RT-PCR data used for quantification of 18S, KCNJ10, KCNQ1 and KCNQ4.
T8572 19606-19607 LS denotes b
T8574 19607-19609 : denotes :
T8573 19609-19616 NN denotes Example
T8575 19617-19619 IN denotes of
T8576 19620-19624 JJ denotes real
T8578 19624-19625 HYPH denotes -
T8577 19625-19629 NN denotes time
T8580 19630-19632 NN denotes RT
T8582 19632-19633 HYPH denotes -
T8581 19633-19636 NN denotes PCR
T8579 19637-19641 NNS denotes data
T8583 19642-19646 VBN denotes used
T8584 19647-19650 IN denotes for
T8585 19651-19665 NN denotes quantification
T8586 19666-19668 IN denotes of
T8587 19669-19672 NN denotes 18S
T8588 19672-19674 , denotes ,
T8589 19674-19680 NN denotes KCNJ10
T8590 19680-19682 , denotes ,
T8591 19682-19687 NN denotes KCNQ1
T8592 19688-19691 CC denotes and
T8593 19692-19697 NN denotes KCNQ4
T8594 19697-19698 . denotes .
T8595 19698-19765 sentence denotes Known quantities of 18S rRNA were used to calibrate the threshold.
T8596 19699-19704 JJ denotes Known
T8597 19705-19715 NNS denotes quantities
T8599 19716-19718 IN denotes of
T8600 19719-19722 NN denotes 18S
T8601 19723-19727 NN denotes rRNA
T8602 19728-19732 VBD denotes were
T8598 19733-19737 VBN denotes used
T8603 19738-19740 TO denotes to
T8604 19741-19750 VB denotes calibrate
T8605 19751-19754 DT denotes the
T8606 19755-19764 NN denotes threshold
T8607 19764-19765 . denotes .
T8608 19765-19807 sentence denotes SV, stria vascularis; SG, spiral ganglia.
T8609 19766-19768 NN denotes SV
T8610 19768-19770 , denotes ,
T8611 19770-19775 NN denotes stria
T8612 19776-19786 NN denotes vascularis
T8613 19786-19787 : denotes ;
T8614 19788-19790 NN denotes SG
T8615 19790-19792 , denotes ,
T8616 19792-19798 JJ denotes spiral
T8617 19799-19806 NNS denotes ganglia
T8618 19806-19807 . denotes .
T8619 19807-19905 sentence denotes c: Quantification of KCNJ10 and KCNQ1 mRNA in young Slc26a4+/+ and young and old Slc26a4-/- mice.
T8620 19808-19809 LS denotes c
T8622 19809-19811 : denotes :
T8621 19811-19825 NN denotes Quantification
T8623 19826-19828 IN denotes of
T8624 19829-19835 NN denotes KCNJ10
T8626 19836-19839 CC denotes and
T8627 19840-19845 NN denotes KCNQ1
T8625 19846-19850 NN denotes mRNA
T8628 19851-19853 IN denotes in
T8629 19854-19859 JJ denotes young
T8630 19860-19867 NN denotes Slc26a4
T8631 19867-19868 SYM denotes +
T8632 19868-19869 HYPH denotes /
T8633 19869-19870 SYM denotes +
T8634 19871-19874 CC denotes and
T8635 19875-19880 JJ denotes young
T8637 19881-19884 CC denotes and
T8638 19885-19888 JJ denotes old
T8639 19889-19896 NN denotes Slc26a4
T8640 19896-19897 SYM denotes -
T8641 19897-19898 HYPH denotes /
T8636 19898-19899 SYM denotes -
T8642 19900-19904 NNS denotes mice
T8643 19904-19905 . denotes .
T6144 19906-19909 DT denotes The
T6143 19906-19911 sentence denotes The p
T6145 19910-19911 NN denotes p
T6147 19911-19912 JJ denotes r
T6146 19911-19916 sentence denotes resen
T6148 19912-19914 NNS denotes es
T6149 19914-19916 VBG denotes en
T6151 19916-19917 NN denotes c
T6150 19916-19922 sentence denotes ce of
T6152 19917-19918 NNS denotes e
T6153 19919-19920 NN denotes o
T6154 19920-19921 IN denotes f
T6156 19922-19928 NN denotes KCNJ10
T6155 19922-19929 sentence denotes KCNJ10
T6157 19928-19929 , denotes
T6159 19929-19933 NN denotes mRNA
T6158 19929-19937 sentence denotes mRNA in
T6160 19934-19936 IN denotes in
T6162 19937-19938 NN denotes s
T6161 19937-20030 sentence denotes stria vascularis of Slc26a4-/- mice supports the finding that intermediate cells are present.
T6164 19938-19942 NN denotes tria
T6165 19943-19953 NN denotes vascularis
T6166 19954-19956 IN denotes of
T6167 19957-19964 NN denotes Slc26a4
T6169 19964-19965 SYM denotes -
T6170 19965-19966 HYPH denotes /
T6171 19966-19967 SYM denotes -
T6168 19968-19972 NNS denotes mice
T6163 19973-19981 VBZ denotes supports
T6172 19982-19985 DT denotes the
T6173 19986-19993 NN denotes finding
T6174 19994-19998 IN denotes that
T6176 19999-20011 JJ denotes intermediate
T6177 20012-20017 NNS denotes cells
T6175 20018-20021 VBP denotes are
T6178 20022-20029 JJ denotes present
T6179 20029-20030 . denotes .
T6180 20030-20134 sentence denotes Expression of the KCNJ10 protein was assessed in young Slc26a4-/- mice by confocal immunocytochemistry.
T6181 20031-20041 NN denotes Expression
T6183 20042-20044 IN denotes of
T6184 20045-20048 DT denotes the
T6186 20049-20055 NN denotes KCNJ10
T6185 20056-20063 NN denotes protein
T6187 20064-20067 VBD denotes was
T6182 20068-20076 VBN denotes assessed
T6188 20077-20079 IN denotes in
T6189 20080-20085 JJ denotes young
T6191 20086-20093 NN denotes Slc26a4
T6192 20093-20094 SYM denotes -
T6193 20094-20095 HYPH denotes /
T6194 20095-20096 SYM denotes -
T6190 20097-20101 NNS denotes mice
T6195 20102-20104 IN denotes by
T6196 20105-20113 JJ denotes confocal
T6197 20114-20133 NN denotes immunocytochemistry
T6198 20133-20134 . denotes .
T6199 20134-20252 sentence denotes Interestingly, expression of the KCNJ10 protein was absent in stria vascularis but normal in spiral ganglia (Fig. 4).
T6200 20135-20148 RB denotes Interestingly
T6202 20148-20150 , denotes ,
T6203 20150-20160 NN denotes expression
T6204 20161-20163 IN denotes of
T6205 20164-20167 DT denotes the
T6207 20168-20174 NN denotes KCNJ10
T6206 20175-20182 NN denotes protein
T6201 20183-20186 VBD denotes was
T6208 20187-20193 JJ denotes absent
T6209 20194-20196 IN denotes in
T6210 20197-20202 NN denotes stria
T6211 20203-20213 NN denotes vascularis
T6212 20214-20217 CC denotes but
T6213 20218-20224 JJ denotes normal
T6214 20225-20227 IN denotes in
T6215 20228-20234 JJ denotes spiral
T6216 20235-20242 NNS denotes ganglia
T6217 20243-20244 -LRB- denotes (
T6218 20244-20248 NN denotes Fig.
T6219 20249-20250 CD denotes 4
T6220 20250-20251 -RRB- denotes )
T6221 20251-20252 . denotes .
T6222 20252-20379 sentence denotes The absence of the KCNJ10 K+ channel in stria vascularis is sufficient to explain the loss of the endocochlear potential [17].
T6223 20253-20256 DT denotes The
T6224 20257-20264 NN denotes absence
T6226 20265-20267 IN denotes of
T6227 20268-20271 DT denotes the
T6229 20272-20278 NN denotes KCNJ10
T6230 20279-20280 NN denotes K
T6231 20280-20281 SYM denotes +
T6228 20282-20289 NN denotes channel
T6232 20290-20292 IN denotes in
T6233 20293-20298 NN denotes stria
T6234 20299-20309 NN denotes vascularis
T6225 20310-20312 VBZ denotes is
T6235 20313-20323 JJ denotes sufficient
T6236 20324-20326 TO denotes to
T6237 20327-20334 VB denotes explain
T6238 20335-20338 DT denotes the
T6239 20339-20343 NN denotes loss
T6240 20344-20346 IN denotes of
T6241 20347-20350 DT denotes the
T6243 20351-20363 JJ denotes endocochlear
T6242 20364-20373 NN denotes potential
T6244 20374-20375 -LRB- denotes [
T6245 20375-20377 CD denotes 17
T6246 20377-20378 -RRB- denotes ]
T6247 20378-20379 . denotes .
T6248 20379-20380 sentence denotes
T8717 20390-20397 NN denotes Protein
T8718 20398-20410 NN denotes localization
T8719 20411-20413 IN denotes of
T8720 20414-20420 NN denotes KCNJ10
T8721 20421-20423 IN denotes in
T8722 20424-20427 DT denotes the
T8723 20428-20435 NN denotes cochlea
T8724 20436-20438 IN denotes of
T8725 20439-20446 NN denotes Slc26a4
T8727 20446-20447 SYM denotes +
T8728 20447-20448 HYPH denotes /
T8729 20448-20449 SYM denotes +
T8730 20450-20453 CC denotes and
T8731 20454-20461 NN denotes Slc26a4
T8732 20461-20462 SYM denotes -
T8733 20462-20463 HYPH denotes /
T8734 20463-20464 SYM denotes -
T8726 20465-20469 NNS denotes mice
T8735 20469-20470 . denotes .
T8736 20470-20508 sentence denotes a: Overview of cochlea; bar = 100 μm.
T8737 20471-20472 LS denotes a
T8739 20472-20474 : denotes :
T8740 20474-20482 NN denotes Overview
T8741 20483-20485 IN denotes of
T8742 20486-20493 NN denotes cochlea
T8743 20493-20494 : denotes ;
T8744 20495-20498 NN denotes bar
T8745 20499-20500 SYM denotes =
T8746 20501-20504 CD denotes 100
T8738 20505-20507 NNS denotes μm
T8747 20507-20508 . denotes .
T8748 20508-20599 sentence denotes Compare to Fig. 1a to note the enlarged scala media and the distended Reissner's membrane.
T8749 20509-20516 VB denotes Compare
T8750 20517-20519 IN denotes to
T8751 20520-20524 NN denotes Fig.
T8752 20525-20527 CD denotes 1a
T8753 20528-20530 TO denotes to
T8754 20531-20535 VB denotes note
T8755 20536-20539 DT denotes the
T8757 20540-20548 JJ denotes enlarged
T8758 20549-20554 NN denotes scala
T8756 20555-20560 NNS denotes media
T8759 20561-20564 CC denotes and
T8760 20565-20568 DT denotes the
T8762 20569-20578 JJ denotes distended
T8763 20579-20587 NNP denotes Reissner
T8764 20587-20589 POS denotes 's
T8761 20590-20598 NN denotes membrane
T8765 20598-20599 . denotes .
T8766 20599-20687 sentence denotes b-c: Detail of lateral wall and spiral ganglia (insert); main bar: 10 μm, insert: 5 μm.
T8767 20600-20601 LS denotes b
T8769 20601-20602 HYPH denotes -
T8770 20602-20603 LS denotes c
T8771 20603-20605 : denotes :
T8768 20605-20611 NN denotes Detail
T8772 20612-20614 IN denotes of
T8773 20615-20622 JJ denotes lateral
T8774 20623-20627 NN denotes wall
T8775 20628-20631 CC denotes and
T8776 20632-20638 JJ denotes spiral
T8777 20639-20646 NNS denotes ganglia
T8778 20647-20648 -LRB- denotes (
T8779 20648-20654 NN denotes insert
T8780 20654-20655 -RRB- denotes )
T8781 20655-20656 : denotes ;
T8782 20657-20661 JJ denotes main
T8783 20662-20665 NN denotes bar
T8785 20665-20667 : denotes :
T8786 20667-20669 CD denotes 10
T8784 20670-20672 NNS denotes μm
T8787 20672-20674 , denotes ,
T8788 20674-20680 NN denotes insert
T8790 20680-20682 : denotes :
T8791 20682-20683 CD denotes 5
T8789 20684-20686 NNS denotes μm
T8792 20686-20687 . denotes .
T8793 20687-20798 sentence denotes Expression of KCNJ10 in Slc26a4-/- mice was absent in stria vascularis but unchanged in spiral ganglion cells.
T8794 20688-20698 NN denotes Expression
T8796 20699-20701 IN denotes of
T8797 20702-20708 NN denotes KCNJ10
T8798 20709-20711 IN denotes in
T8799 20712-20719 NN denotes Slc26a4
T8801 20719-20720 SYM denotes -
T8802 20720-20721 HYPH denotes /
T8803 20721-20722 SYM denotes -
T8800 20723-20727 NNS denotes mice
T8795 20728-20731 VBD denotes was
T8804 20732-20738 JJ denotes absent
T8805 20739-20741 IN denotes in
T8806 20742-20747 NN denotes stria
T8807 20748-20758 NN denotes vascularis
T8808 20759-20762 CC denotes but
T8809 20763-20772 JJ denotes unchanged
T8810 20773-20775 IN denotes in
T8811 20776-20782 JJ denotes spiral
T8812 20783-20791 NN denotes ganglion
T8813 20792-20797 NNS denotes cells
T8814 20797-20798 . denotes .
T8815 20798-20930 sentence denotes RM, Reissner's membrane, SV, stria vascularis; SP, spiral prominence; SL, spiral ligament; LIM, spiral limbus; SG, spiral ganglion.
T8816 20799-20801 NNP denotes RM
T8817 20801-20803 , denotes ,
T8818 20803-20811 NNP denotes Reissner
T8820 20811-20813 POS denotes 's
T8819 20814-20822 NN denotes membrane
T8821 20822-20824 , denotes ,
T8822 20824-20826 NN denotes SV
T8823 20826-20828 , denotes ,
T8824 20828-20833 NN denotes stria
T8825 20834-20844 NN denotes vascularis
T8826 20844-20845 : denotes ;
T8827 20846-20848 NN denotes SP
T8828 20848-20850 , denotes ,
T8829 20850-20856 JJ denotes spiral
T8830 20857-20867 NN denotes prominence
T8831 20867-20868 : denotes ;
T8832 20869-20871 NN denotes SL
T8833 20871-20873 , denotes ,
T8834 20873-20879 JJ denotes spiral
T8835 20880-20888 NN denotes ligament
T8836 20888-20889 : denotes ;
T8837 20890-20893 NN denotes LIM
T8838 20893-20895 , denotes ,
T8839 20895-20901 JJ denotes spiral
T8840 20902-20908 NN denotes limbus
T8841 20908-20909 : denotes ;
T8842 20910-20912 NN denotes SG
T8843 20912-20914 , denotes ,
T8844 20914-20920 JJ denotes spiral
T8845 20921-20929 NN denotes ganglion
T8846 20929-20930 . denotes .
T6250 20931-20943 JJ denotes Histological
T6249 20931-21132 sentence denotes Histological evaluation of cryosections revealed an enlargement of scala media with a large bulging of Reissner's membrane and an apparent degeneration of the organ of Corti, as described earlier [14].
T6251 20944-20954 NN denotes evaluation
T6253 20955-20957 IN denotes of
T6254 20958-20970 NNS denotes cryosections
T6252 20971-20979 VBD denotes revealed
T6255 20980-20982 DT denotes an
T6256 20983-20994 NN denotes enlargement
T6257 20995-20997 IN denotes of
T6258 20998-21003 NN denotes scala
T6259 21004-21009 NNS denotes media
T6260 21010-21014 IN denotes with
T6261 21015-21016 DT denotes a
T6263 21017-21022 JJ denotes large
T6262 21023-21030 NN denotes bulging
T6264 21031-21033 IN denotes of
T6265 21034-21042 NNP denotes Reissner
T6267 21042-21044 POS denotes 's
T6266 21045-21053 NN denotes membrane
T6268 21054-21057 CC denotes and
T6269 21058-21060 DT denotes an
T6271 21061-21069 JJ denotes apparent
T6270 21070-21082 NN denotes degeneration
T6272 21083-21085 IN denotes of
T6273 21086-21089 DT denotes the
T6274 21090-21095 NN denotes organ
T6275 21096-21098 IN denotes of
T6276 21099-21104 NNP denotes Corti
T6277 21104-21106 , denotes ,
T6278 21106-21108 IN denotes as
T6279 21109-21118 VBN denotes described
T6280 21119-21126 RBR denotes earlier
T6281 21127-21128 -LRB- denotes [
T6282 21128-21130 CD denotes 14
T6283 21130-21131 -RRB- denotes ]
T6284 21131-21132 . denotes .
T6285 21132-21283 sentence denotes Tissue height of stria vascularis was normal (Fig. 5), consistent with the similar numbers of 18S rRNA molecules in isolated preparations (see above).
T6286 21133-21139 NN denotes Tissue
T6287 21140-21146 NN denotes height
T6289 21147-21149 IN denotes of
T6290 21150-21155 NN denotes stria
T6291 21156-21166 NN denotes vascularis
T6288 21167-21170 VBD denotes was
T6292 21171-21177 JJ denotes normal
T6293 21178-21179 -LRB- denotes (
T6294 21179-21183 NN denotes Fig.
T6295 21184-21185 CD denotes 5
T6296 21185-21186 -RRB- denotes )
T6297 21186-21188 , denotes ,
T6298 21188-21198 JJ denotes consistent
T6299 21199-21203 IN denotes with
T6300 21204-21207 DT denotes the
T6302 21208-21215 JJ denotes similar
T6301 21216-21223 NNS denotes numbers
T6303 21224-21226 IN denotes of
T6304 21227-21230 NN denotes 18S
T6306 21231-21235 NN denotes rRNA
T6305 21236-21245 NNS denotes molecules
T6307 21246-21248 IN denotes in
T6308 21249-21257 VBN denotes isolated
T6309 21258-21270 NNS denotes preparations
T6310 21271-21272 -LRB- denotes (
T6311 21272-21275 VB denotes see
T6312 21276-21281 RB denotes above
T6313 21281-21282 -RRB- denotes )
T6314 21282-21283 . denotes .
T6315 21283-21384 sentence denotes The absence of a change in tissue height is consistent with the presence of intermediate cells [28].
T6316 21284-21287 DT denotes The
T6317 21288-21295 NN denotes absence
T6319 21296-21298 IN denotes of
T6320 21299-21300 DT denotes a
T6321 21301-21307 NN denotes change
T6322 21308-21310 IN denotes in
T6323 21311-21317 NN denotes tissue
T6324 21318-21324 NN denotes height
T6318 21325-21327 VBZ denotes is
T6325 21328-21338 JJ denotes consistent
T6326 21339-21343 IN denotes with
T6327 21344-21347 DT denotes the
T6328 21348-21356 NN denotes presence
T6329 21357-21359 IN denotes of
T6330 21360-21372 JJ denotes intermediate
T6331 21373-21378 NNS denotes cells
T6332 21379-21380 -LRB- denotes [
T6333 21380-21382 CD denotes 28
T6334 21382-21383 -RRB- denotes ]
T6335 21383-21384 . denotes .
T6336 21384-21514 sentence denotes Further, we observed an apparent loss of tissue masses in areas that are normally occupied primarily by type I and II fibrocytes.
T6337 21385-21392 RB denotes Further
T6339 21392-21394 , denotes ,
T6340 21394-21396 PRP denotes we
T6338 21397-21405 VBD denotes observed
T6341 21406-21408 DT denotes an
T6343 21409-21417 JJ denotes apparent
T6342 21418-21422 NN denotes loss
T6344 21423-21425 IN denotes of
T6345 21426-21432 NN denotes tissue
T6346 21433-21439 NNS denotes masses
T6347 21440-21442 IN denotes in
T6348 21443-21448 NNS denotes areas
T6349 21449-21453 WDT denotes that
T6351 21454-21457 VBP denotes are
T6352 21458-21466 RB denotes normally
T6350 21467-21475 VBN denotes occupied
T6353 21476-21485 RB denotes primarily
T6354 21486-21488 IN denotes by
T6355 21489-21493 NN denotes type
T6356 21494-21495 CD denotes I
T6358 21496-21499 CC denotes and
T6359 21500-21502 CD denotes II
T6357 21503-21513 NNS denotes fibrocytes
T6360 21513-21514 . denotes .
T6361 21514-21631 sentence denotes Spiral prominence in Slc26a4-/- mice was less prominent, spiral ligament thinner and spiral limbus flatter (Fig. 5).
T6362 21515-21521 JJ denotes Spiral
T6363 21522-21532 NN denotes prominence
T6365 21533-21535 IN denotes in
T6366 21536-21543 NN denotes Slc26a4
T6368 21543-21544 SYM denotes -
T6369 21544-21545 HYPH denotes /
T6370 21545-21546 SYM denotes -
T6367 21547-21551 NNS denotes mice
T6364 21552-21555 VBD denotes was
T6371 21556-21560 RBR denotes less
T6372 21561-21570 JJ denotes prominent
T6373 21570-21572 , denotes ,
T6374 21572-21578 JJ denotes spiral
T6375 21579-21587 NN denotes ligament
T6376 21588-21595 JJR denotes thinner
T6377 21596-21599 CC denotes and
T6378 21600-21606 JJ denotes spiral
T6379 21607-21613 NN denotes limbus
T6380 21614-21621 JJR denotes flatter
T6381 21622-21623 -LRB- denotes (
T6382 21623-21627 NN denotes Fig.
T6383 21628-21629 CD denotes 5
T6384 21629-21630 -RRB- denotes )
T6385 21630-21631 . denotes .
T6386 21631-21824 sentence denotes The observation that tissue masses were lost in the spiral limbus region is consistent with the finding of a reduced number of 18S rRNA molecules in the spiral ganglia preparation (see above).
T6387 21632-21635 DT denotes The
T6388 21636-21647 NN denotes observation
T6390 21648-21652 IN denotes that
T6392 21653-21659 NN denotes tissue
T6393 21660-21666 NNS denotes masses
T6394 21667-21671 VBD denotes were
T6391 21672-21676 VBN denotes lost
T6395 21677-21679 IN denotes in
T6396 21680-21683 DT denotes the
T6398 21684-21690 JJ denotes spiral
T6399 21691-21697 NN denotes limbus
T6397 21698-21704 NN denotes region
T6389 21705-21707 VBZ denotes is
T6400 21708-21718 JJ denotes consistent
T6401 21719-21723 IN denotes with
T6402 21724-21727 DT denotes the
T6403 21728-21735 NN denotes finding
T6404 21736-21738 IN denotes of
T6405 21739-21740 DT denotes a
T6407 21741-21748 VBN denotes reduced
T6406 21749-21755 NN denotes number
T6408 21756-21758 IN denotes of
T6409 21759-21762 NN denotes 18S
T6410 21763-21767 NN denotes rRNA
T6411 21768-21777 NNS denotes molecules
T6412 21778-21780 IN denotes in
T6413 21781-21784 DT denotes the
T6415 21785-21791 JJ denotes spiral
T6416 21792-21799 NNS denotes ganglia
T6414 21800-21811 NN denotes preparation
T6417 21812-21813 -LRB- denotes (
T6418 21813-21816 VB denotes see
T6419 21817-21822 RB denotes above
T6420 21822-21823 -RRB- denotes )
T6421 21823-21824 . denotes .
T6422 21824-22015 sentence denotes In addition, we observed an apparent degeneration of stria vascularis, including an increase in pigmentation and an irregular pattern of the tight junctions of marginal cells (Fig. 2c, 6,7).
T6423 21825-21827 IN denotes In
T6425 21828-21836 NN denotes addition
T6426 21836-21838 , denotes ,
T6427 21838-21840 PRP denotes we
T6424 21841-21849 VBD denotes observed
T6428 21850-21852 DT denotes an
T6430 21853-21861 JJ denotes apparent
T6429 21862-21874 NN denotes degeneration
T6431 21875-21877 IN denotes of
T6432 21878-21883 NN denotes stria
T6433 21884-21894 NN denotes vascularis
T6434 21894-21896 , denotes ,
T6435 21896-21905 VBG denotes including
T6436 21906-21908 DT denotes an
T6437 21909-21917 NN denotes increase
T6438 21918-21920 IN denotes in
T6439 21921-21933 NN denotes pigmentation
T6440 21934-21937 CC denotes and
T6441 21938-21940 DT denotes an
T6443 21941-21950 JJ denotes irregular
T6442 21951-21958 NN denotes pattern
T6444 21959-21961 IN denotes of
T6445 21962-21965 DT denotes the
T6447 21966-21971 JJ denotes tight
T6446 21972-21981 NNS denotes junctions
T6448 21982-21984 IN denotes of
T6449 21985-21993 JJ denotes marginal
T6450 21994-21999 NNS denotes cells
T6451 22000-22001 -LRB- denotes (
T6453 22001-22005 NN denotes Fig.
T6454 22006-22008 CD denotes 2c
T6455 22008-22010 , denotes ,
T6456 22010-22011 CD denotes 6
T6457 22011-22012 , denotes ,
T6452 22012-22013 CD denotes 7
T6458 22013-22014 -RRB- denotes )
T6459 22014-22015 . denotes .
T6460 22015-22070 sentence denotes Tight junctions were visualized by F-actin expression.
T6461 22016-22021 JJ denotes Tight
T6462 22022-22031 NNS denotes junctions
T6464 22032-22036 VBD denotes were
T6463 22037-22047 VBN denotes visualized
T6465 22048-22050 IN denotes by
T6466 22051-22052 NN denotes F
T6468 22052-22053 HYPH denotes -
T6467 22053-22058 NN denotes actin
T6469 22059-22069 NN denotes expression
T6470 22069-22070 . denotes .
T6471 22070-22122 sentence denotes Marginal cells appeared to form a continuous layer.
T6472 22071-22079 JJ denotes Marginal
T6473 22080-22085 NNS denotes cells
T6474 22086-22094 VBD denotes appeared
T6475 22095-22097 TO denotes to
T6476 22098-22102 VB denotes form
T6477 22103-22104 DT denotes a
T6479 22105-22115 JJ denotes continuous
T6478 22116-22121 NN denotes layer
T6480 22121-22122 . denotes .
T6481 22122-22123 sentence denotes
T8911 22133-22145 JJ denotes Morphometric
T8912 22146-22154 NN denotes analysis
T8913 22155-22157 IN denotes of
T8914 22158-22166 JJ denotes cochlear
T8916 22167-22173 NN denotes tissue
T8915 22174-22180 NNS denotes masses
T8917 22181-22183 IN denotes in
T8918 22184-22191 NN denotes Slc26a4
T8920 22191-22192 SYM denotes +
T8921 22192-22193 HYPH denotes /
T8922 22193-22194 SYM denotes +
T8923 22195-22198 CC denotes and
T8924 22199-22206 NN denotes Slc26a4
T8925 22206-22207 SYM denotes -
T8926 22207-22208 HYPH denotes /
T8927 22208-22209 SYM denotes -
T8919 22210-22214 NNS denotes mice
T8928 22214-22215 . denotes .
T8929 22215-22245 sentence denotes a: locations of measurements.
T8930 22216-22217 LS denotes a
T8932 22217-22219 : denotes :
T8931 22219-22228 NNS denotes locations
T8933 22229-22231 IN denotes of
T8934 22232-22244 NNS denotes measurements
T8935 22244-22245 . denotes .
T8936 22245-22383 sentence denotes Thickness of stria vascularis (SV) was obtained as average of three distance measurements perpendicular to the surface of marginal cells.
T8937 22246-22255 NN denotes Thickness
T8939 22256-22258 IN denotes of
T8940 22259-22264 NN denotes stria
T8941 22265-22275 NN denotes vascularis
T8942 22276-22277 -LRB- denotes (
T8943 22277-22279 NN denotes SV
T8944 22279-22280 -RRB- denotes )
T8945 22281-22284 VBD denotes was
T8938 22285-22293 VBN denotes obtained
T8946 22294-22296 IN denotes as
T8947 22297-22304 NN denotes average
T8948 22305-22307 IN denotes of
T8949 22308-22313 CD denotes three
T8951 22314-22322 NN denotes distance
T8950 22323-22335 NNS denotes measurements
T8952 22336-22349 JJ denotes perpendicular
T8953 22350-22352 IN denotes to
T8954 22353-22356 DT denotes the
T8955 22357-22364 NN denotes surface
T8956 22365-22367 IN denotes of
T8957 22368-22376 JJ denotes marginal
T8958 22377-22382 NNS denotes cells
T8959 22382-22383 . denotes .
T8960 22383-22569 sentence denotes Thickness of spiral prominence (SP) was measured perpendicular to a tangential line (dashed) that connects the surface of the outer sulcus (OS) with the basal layer of stria vascularis.
T8961 22384-22393 NN denotes Thickness
T8963 22394-22396 IN denotes of
T8964 22397-22403 JJ denotes spiral
T8965 22404-22414 NN denotes prominence
T8966 22415-22416 -LRB- denotes (
T8967 22416-22418 NN denotes SP
T8968 22418-22419 -RRB- denotes )
T8969 22420-22423 VBD denotes was
T8962 22424-22432 VBN denotes measured
T8970 22433-22446 JJ denotes perpendicular
T8971 22447-22449 IN denotes to
T8972 22450-22451 DT denotes a
T8974 22452-22462 JJ denotes tangential
T8973 22463-22467 NN denotes line
T8975 22468-22469 -LRB- denotes (
T8976 22469-22475 JJ denotes dashed
T8977 22475-22476 -RRB- denotes )
T8978 22477-22481 WDT denotes that
T8979 22482-22490 VBZ denotes connects
T8980 22491-22494 DT denotes the
T8981 22495-22502 NN denotes surface
T8982 22503-22505 IN denotes of
T8983 22506-22509 DT denotes the
T8985 22510-22515 JJ denotes outer
T8984 22516-22522 NN denotes sulcus
T8986 22523-22524 -LRB- denotes (
T8987 22524-22526 NN denotes OS
T8988 22526-22527 -RRB- denotes )
T8989 22528-22532 IN denotes with
T8990 22533-22536 DT denotes the
T8992 22537-22542 JJ denotes basal
T8991 22543-22548 NN denotes layer
T8993 22549-22551 IN denotes of
T8994 22552-22557 NN denotes stria
T8995 22558-22568 NN denotes vascularis
T8996 22568-22569 . denotes .
T8997 22569-22762 sentence denotes Thickness of spiral ligament (SL) was measured perpendicular to the tangential line as distance between the surface of spiral prominence and the interface between spiral ligament and bone (B).
T8998 22570-22579 NN denotes Thickness
T9000 22580-22582 IN denotes of
T9001 22583-22589 JJ denotes spiral
T9002 22590-22598 NN denotes ligament
T9003 22599-22600 -LRB- denotes (
T9004 22600-22602 NN denotes SL
T9005 22602-22603 -RRB- denotes )
T9006 22604-22607 VBD denotes was
T8999 22608-22616 VBN denotes measured
T9007 22617-22630 JJ denotes perpendicular
T9008 22631-22633 IN denotes to
T9009 22634-22637 DT denotes the
T9011 22638-22648 JJ denotes tangential
T9010 22649-22653 NN denotes line
T9012 22654-22656 IN denotes as
T9013 22657-22665 NN denotes distance
T9014 22666-22673 IN denotes between
T9015 22674-22677 DT denotes the
T9016 22678-22685 NN denotes surface
T9017 22686-22688 IN denotes of
T9018 22689-22695 JJ denotes spiral
T9019 22696-22706 NN denotes prominence
T9020 22707-22710 CC denotes and
T9021 22711-22714 DT denotes the
T9022 22715-22724 NN denotes interface
T9023 22725-22732 IN denotes between
T9024 22733-22739 JJ denotes spiral
T9025 22740-22748 NN denotes ligament
T9026 22749-22752 CC denotes and
T9027 22753-22757 NN denotes bone
T9028 22758-22759 -LRB- denotes (
T9029 22759-22760 NN denotes B
T9030 22760-22761 -RRB- denotes )
T9031 22761-22762 . denotes .
T9032 22762-23022 sentence denotes Thickness of spiral limbus (LIM) was obtained perpendicular to the surface of the bone as a tangential line that touches the inner sulcus (IS) and reaches from the surface of the spiral limbus to the interface between spiral limbus connective tissue and bone.
T9033 22763-22772 NN denotes Thickness
T9035 22773-22775 IN denotes of
T9036 22776-22782 JJ denotes spiral
T9037 22783-22789 NN denotes limbus
T9038 22790-22791 -LRB- denotes (
T9039 22791-22794 NN denotes LIM
T9040 22794-22795 -RRB- denotes )
T9041 22796-22799 VBD denotes was
T9034 22800-22808 VBN denotes obtained
T9042 22809-22822 JJ denotes perpendicular
T9043 22823-22825 IN denotes to
T9044 22826-22829 DT denotes the
T9045 22830-22837 NN denotes surface
T9046 22838-22840 IN denotes of
T9047 22841-22844 DT denotes the
T9048 22845-22849 NN denotes bone
T9049 22850-22852 IN denotes as
T9050 22853-22854 DT denotes a
T9052 22855-22865 JJ denotes tangential
T9051 22866-22870 NN denotes line
T9053 22871-22875 WDT denotes that
T9054 22876-22883 VBZ denotes touches
T9055 22884-22887 DT denotes the
T9057 22888-22893 JJ denotes inner
T9056 22894-22900 NN denotes sulcus
T9058 22901-22902 -LRB- denotes (
T9059 22902-22904 NN denotes IS
T9060 22904-22905 -RRB- denotes )
T9061 22906-22909 CC denotes and
T9062 22910-22917 VBZ denotes reaches
T9063 22918-22922 IN denotes from
T9064 22923-22926 DT denotes the
T9065 22927-22934 NN denotes surface
T9066 22935-22937 IN denotes of
T9067 22938-22941 DT denotes the
T9069 22942-22948 JJ denotes spiral
T9068 22949-22955 NN denotes limbus
T9070 22956-22958 IN denotes to
T9071 22959-22962 DT denotes the
T9072 22963-22972 NN denotes interface
T9073 22973-22980 IN denotes between
T9074 22981-22987 JJ denotes spiral
T9075 22988-22994 NN denotes limbus
T9076 22995-23005 JJ denotes connective
T9077 23006-23012 NN denotes tissue
T9078 23013-23016 CC denotes and
T9079 23017-23021 NN denotes bone
T9080 23021-23022 . denotes .
T9081 23022-23034 sentence denotes b: Summary.
T9082 23023-23024 LS denotes b
T9084 23024-23026 : denotes :
T9083 23026-23033 NN denotes Summary
T9085 23033-23034 . denotes .
T9086 23034-23084 sentence denotes Data from 7–8 animals contributed to each column.
T9087 23035-23039 NNS denotes Data
T9089 23040-23044 IN denotes from
T9090 23045-23046 CD denotes 7
T9092 23046-23047 SYM denotes –
T9091 23047-23048 CD denotes 8
T9093 23049-23056 NNS denotes animals
T9088 23057-23068 VBD denotes contributed
T9094 23069-23071 IN denotes to
T9095 23072-23076 DT denotes each
T9096 23077-23083 NN denotes column
T9097 23083-23084 . denotes .
T9176 23095-23103 NN denotes Analysis
T9177 23104-23106 IN denotes of
T9178 23107-23115 JJ denotes marginal
T9180 23116-23119 CC denotes and
T9181 23120-23125 JJ denotes basal
T9179 23126-23130 NN denotes cell
T9182 23131-23139 NNS denotes barriers
T9183 23140-23142 IN denotes by
T9184 23143-23145 IN denotes in
T9185 23146-23153 NN denotes Slc26a4
T9187 23153-23154 SYM denotes -
T9188 23154-23155 HYPH denotes /
T9189 23155-23156 SYM denotes -
T9186 23157-23161 NNS denotes mice
T9190 23161-23162 . denotes .
T9191 23162-23206 sentence denotes Tight junctions were visualized by F actin.
T9192 23163-23168 JJ denotes Tight
T9193 23169-23178 NNS denotes junctions
T9195 23179-23183 VBD denotes were
T9194 23184-23194 VBN denotes visualized
T9196 23195-23197 IN denotes by
T9197 23198-23199 NN denotes F
T9198 23200-23205 NN denotes actin
T9199 23205-23206 . denotes .
T9200 23206-23327 sentence denotes Whole-mounts of stria vascularis were viewed either from the basal cell side (a-f) or from the marginal cell side (g-l).
T9201 23207-23212 JJ denotes Whole
T9203 23212-23213 HYPH denotes -
T9202 23213-23219 NNS denotes mounts
T9205 23220-23222 IN denotes of
T9206 23223-23228 NN denotes stria
T9207 23229-23239 NN denotes vascularis
T9208 23240-23244 VBD denotes were
T9204 23245-23251 VBN denotes viewed
T9209 23252-23258 CC denotes either
T9210 23259-23263 IN denotes from
T9211 23264-23267 DT denotes the
T9213 23268-23273 JJ denotes basal
T9214 23274-23278 NN denotes cell
T9212 23279-23283 NN denotes side
T9215 23284-23285 -LRB- denotes (
T9216 23285-23286 NN denotes a
T9217 23286-23287 SYM denotes -
T9218 23287-23288 NN denotes f
T9219 23288-23289 -RRB- denotes )
T9220 23290-23292 CC denotes or
T9221 23293-23297 IN denotes from
T9222 23298-23301 DT denotes the
T9224 23302-23310 JJ denotes marginal
T9225 23311-23315 NN denotes cell
T9223 23316-23320 NN denotes side
T9226 23321-23322 -LRB- denotes (
T9227 23322-23323 NN denotes g
T9228 23323-23324 SYM denotes -
T9229 23324-23325 NN denotes l
T9230 23325-23326 -RRB- denotes )
T9231 23326-23327 . denotes .
T9232 23327-23412 sentence denotes Bright field images verify that the same area was viewed from either side (b and h).
T9233 23328-23334 JJ denotes Bright
T9234 23335-23340 NN denotes field
T9235 23341-23347 NNS denotes images
T9236 23348-23354 VBP denotes verify
T9237 23355-23359 IN denotes that
T9239 23360-23363 DT denotes the
T9241 23364-23368 JJ denotes same
T9240 23369-23373 NN denotes area
T9242 23374-23377 VBD denotes was
T9238 23378-23384 VBN denotes viewed
T9243 23385-23389 IN denotes from
T9244 23390-23396 CC denotes either
T9245 23397-23401 NN denotes side
T9246 23402-23403 -LRB- denotes (
T9247 23403-23404 NN denotes b
T9248 23405-23408 CC denotes and
T9249 23409-23410 NN denotes h
T9250 23410-23411 -RRB- denotes )
T9251 23411-23412 . denotes .
T9252 23412-23545 sentence denotes Colored bright field images were mixed with images of F actin staining to indicate the position of pigment granules (d, j, f and l).
T9253 23413-23420 JJ denotes Colored
T9255 23421-23427 JJ denotes bright
T9256 23428-23433 NN denotes field
T9254 23434-23440 NNS denotes images
T9258 23441-23445 VBD denotes were
T9257 23446-23451 VBN denotes mixed
T9259 23452-23456 IN denotes with
T9260 23457-23463 NNS denotes images
T9261 23464-23466 IN denotes of
T9262 23467-23468 NN denotes F
T9263 23469-23474 NN denotes actin
T9264 23475-23483 NN denotes staining
T9265 23484-23486 TO denotes to
T9266 23487-23495 VB denotes indicate
T9267 23496-23499 DT denotes the
T9268 23500-23508 NN denotes position
T9269 23509-23511 IN denotes of
T9270 23512-23519 NN denotes pigment
T9271 23520-23528 NNS denotes granules
T9272 23529-23530 -LRB- denotes (
T9273 23530-23531 NN denotes d
T9274 23531-23533 , denotes ,
T9275 23533-23534 NN denotes j
T9276 23534-23536 , denotes ,
T9277 23536-23537 NN denotes f
T9278 23538-23541 CC denotes and
T9279 23542-23543 NN denotes l
T9280 23543-23544 -RRB- denotes )
T9281 23544-23545 . denotes .
T9282 23545-23672 sentence denotes Focus was varied to either visualize the marginal cell barrier (SMC, c-d and i-j) or the basal cell barrier (BC, e-f and k-l).
T9283 23546-23551 NN denotes Focus
T9285 23552-23555 VBD denotes was
T9284 23556-23562 VBN denotes varied
T9286 23563-23565 TO denotes to
T9288 23566-23572 CC denotes either
T9287 23573-23582 VB denotes visualize
T9289 23583-23586 DT denotes the
T9291 23587-23595 JJ denotes marginal
T9292 23596-23600 NN denotes cell
T9290 23601-23608 NN denotes barrier
T9293 23609-23610 -LRB- denotes (
T9295 23610-23613 NN denotes SMC
T9296 23613-23615 , denotes ,
T9294 23615-23616 NN denotes c
T9297 23616-23617 SYM denotes -
T9298 23617-23618 NN denotes d
T9299 23619-23622 CC denotes and
T9300 23623-23624 NN denotes i
T9301 23624-23625 SYM denotes -
T9302 23625-23626 NN denotes j
T9303 23626-23627 -RRB- denotes )
T9304 23628-23630 CC denotes or
T9305 23631-23634 DT denotes the
T9307 23635-23640 JJ denotes basal
T9308 23641-23645 NN denotes cell
T9306 23646-23653 NN denotes barrier
T9309 23654-23655 -LRB- denotes (
T9311 23655-23657 NN denotes BC
T9312 23657-23659 , denotes ,
T9310 23659-23660 NN denotes e
T9313 23660-23661 SYM denotes -
T9314 23661-23662 NN denotes f
T9315 23663-23666 CC denotes and
T9316 23667-23668 NN denotes k
T9317 23668-23669 SYM denotes -
T9318 23669-23670 NN denotes l
T9319 23670-23671 -RRB- denotes )
T9320 23671-23672 . denotes .
T9321 23672-23757 sentence denotes Both the marginal cell (e-f) and the basal cell barrier (i-f) appeared to be intact.
T9322 23673-23677 CC denotes Both
T9324 23678-23681 DT denotes the
T9325 23682-23690 JJ denotes marginal
T9323 23691-23695 NN denotes cell
T9327 23696-23697 -LRB- denotes (
T9328 23697-23698 NN denotes e
T9329 23698-23699 SYM denotes -
T9330 23699-23700 NN denotes f
T9331 23700-23701 -RRB- denotes )
T9332 23702-23705 CC denotes and
T9333 23706-23709 DT denotes the
T9335 23710-23715 JJ denotes basal
T9334 23716-23720 NN denotes cell
T9336 23721-23728 NN denotes barrier
T9337 23729-23730 -LRB- denotes (
T9338 23730-23731 NN denotes i
T9339 23731-23732 SYM denotes -
T9340 23732-23733 NN denotes f
T9341 23733-23734 -RRB- denotes )
T9326 23735-23743 VBD denotes appeared
T9342 23744-23746 TO denotes to
T9343 23747-23749 VB denotes be
T9344 23750-23756 JJ denotes intact
T9345 23756-23757 . denotes .
T9346 23757-23845 sentence denotes It was critical for this finding that pigmentation did not block the path of the laser.
T9347 23758-23760 PRP denotes It
T9348 23761-23764 VBD denotes was
T9349 23765-23773 JJ denotes critical
T9350 23774-23777 IN denotes for
T9351 23778-23782 DT denotes this
T9352 23783-23790 NN denotes finding
T9353 23791-23795 IN denotes that
T9355 23796-23808 NN denotes pigmentation
T9356 23809-23812 VBD denotes did
T9357 23813-23816 RB denotes not
T9354 23817-23822 VB denotes block
T9358 23823-23826 DT denotes the
T9359 23827-23831 NN denotes path
T9360 23832-23834 IN denotes of
T9361 23835-23838 DT denotes the
T9362 23839-23844 NN denotes laser
T9363 23844-23845 . denotes .
T9364 23845-23990 sentence denotes Blockage of the laser by pigmentation produces the untrue impression of a discontinuous marginal cell barrier (c-d) or basal cell barrier (k-l).
T9365 23846-23854 NN denotes Blockage
T9367 23855-23857 IN denotes of
T9368 23858-23861 DT denotes the
T9369 23862-23867 NN denotes laser
T9370 23868-23870 IN denotes by
T9371 23871-23883 NN denotes pigmentation
T9366 23884-23892 VBZ denotes produces
T9372 23893-23896 DT denotes the
T9374 23897-23903 JJ denotes untrue
T9373 23904-23914 NN denotes impression
T9375 23915-23917 IN denotes of
T9376 23918-23919 DT denotes a
T9378 23920-23933 JJ denotes discontinuous
T9379 23934-23942 JJ denotes marginal
T9380 23943-23947 NN denotes cell
T9377 23948-23955 NN denotes barrier
T9381 23956-23957 -LRB- denotes (
T9382 23957-23958 NN denotes c
T9383 23958-23959 SYM denotes -
T9384 23959-23960 NN denotes d
T9385 23960-23961 -RRB- denotes )
T9386 23962-23964 CC denotes or
T9387 23965-23970 JJ denotes basal
T9388 23971-23975 NN denotes cell
T9389 23976-23983 NN denotes barrier
T9390 23984-23985 -LRB- denotes (
T9391 23985-23986 NN denotes k
T9392 23986-23987 SYM denotes -
T9393 23987-23988 NN denotes l
T9394 23988-23989 -RRB- denotes )
T9395 23989-23990 . denotes .
T9396 23990-24063 sentence denotes Comparison of images is aided by marking a significant area with a star.
T9397 23991-24001 NN denotes Comparison
T9399 24002-24004 IN denotes of
T9400 24005-24011 NNS denotes images
T9401 24012-24014 VBZ denotes is
T9398 24015-24020 VBN denotes aided
T9402 24021-24023 IN denotes by
T9403 24024-24031 VBG denotes marking
T9404 24032-24033 DT denotes a
T9406 24034-24045 JJ denotes significant
T9405 24046-24050 NN denotes area
T9407 24051-24055 IN denotes with
T9408 24056-24057 DT denotes a
T9409 24058-24062 NN denotes star
T9410 24062-24063 . denotes .
T9411 24063-24077 sentence denotes Bars = 10 μm.
T9412 24064-24068 NNS denotes Bars
T9414 24069-24070 SYM denotes =
T9415 24071-24073 CD denotes 10
T9413 24074-24076 NNS denotes μm
T9416 24076-24077 . denotes .
T9536 24088-24096 NN denotes Analysis
T9537 24097-24099 IN denotes of
T9538 24100-24108 JJ denotes marginal
T9540 24109-24112 CC denotes and
T9541 24113-24118 JJ denotes basal
T9542 24119-24123 NN denotes cell
T9539 24124-24132 NNS denotes barriers
T9543 24133-24135 IN denotes in
T9544 24136-24143 NN denotes Slc26a4
T9546 24143-24144 SYM denotes +
T9547 24144-24145 HYPH denotes /
T9548 24145-24146 SYM denotes +
T9549 24147-24150 CC denotes and
T9550 24151-24158 NN denotes Slc26a4
T9551 24158-24159 SYM denotes -
T9552 24159-24160 HYPH denotes /
T9553 24160-24161 SYM denotes -
T9545 24162-24166 NNS denotes mice
T9554 24166-24167 . denotes .
T9555 24167-24247 sentence denotes Tight junctions were visualized by F actin in whole-mounts of stria vascularis.
T9556 24168-24173 JJ denotes Tight
T9557 24174-24183 NNS denotes junctions
T9559 24184-24188 VBD denotes were
T9558 24189-24199 VBN denotes visualized
T9560 24200-24202 IN denotes by
T9561 24203-24204 NN denotes F
T9562 24205-24210 NN denotes actin
T9563 24211-24213 IN denotes in
T9564 24214-24219 JJ denotes whole
T9566 24219-24220 HYPH denotes -
T9565 24220-24226 NNS denotes mounts
T9567 24227-24229 IN denotes of
T9568 24230-24235 NN denotes stria
T9569 24236-24246 NN denotes vascularis
T9570 24246-24247 . denotes .
T9571 24247-24317 sentence denotes Bright field images were taken to evaluate pigmentation (a, d and g).
T9572 24248-24254 JJ denotes Bright
T9573 24255-24260 NN denotes field
T9574 24261-24267 NNS denotes images
T9576 24268-24272 VBD denotes were
T9575 24273-24278 VBN denotes taken
T9577 24279-24281 TO denotes to
T9578 24282-24290 VB denotes evaluate
T9579 24291-24303 NN denotes pigmentation
T9580 24304-24305 -LRB- denotes (
T9581 24305-24306 NN denotes a
T9582 24306-24308 , denotes ,
T9583 24308-24309 NN denotes d
T9584 24310-24313 CC denotes and
T9585 24314-24315 NN denotes g
T9586 24315-24316 -RRB- denotes )
T9587 24316-24317 . denotes .
T9588 24317-24399 sentence denotes Note the intact marginal cell (b) and basal cell (c) barriers in Slc26a4+/+ mice.
T9589 24318-24322 VB denotes Note
T9590 24323-24326 DT denotes the
T9592 24327-24333 JJ denotes intact
T9593 24334-24342 JJ denotes marginal
T9594 24343-24347 NN denotes cell
T9595 24348-24349 -LRB- denotes (
T9596 24349-24350 NN denotes b
T9597 24350-24351 -RRB- denotes )
T9598 24352-24355 CC denotes and
T9599 24356-24361 JJ denotes basal
T9600 24362-24366 NN denotes cell
T9601 24367-24368 -LRB- denotes (
T9602 24368-24369 NN denotes c
T9603 24369-24370 -RRB- denotes )
T9591 24371-24379 NNS denotes barriers
T9604 24380-24382 IN denotes in
T9605 24383-24390 NN denotes Slc26a4
T9607 24390-24391 SYM denotes +
T9608 24391-24392 HYPH denotes /
T9609 24392-24393 SYM denotes +
T9606 24394-24398 NNS denotes mice
T9610 24398-24399 . denotes .
T9611 24399-24480 sentence denotes Minimal pigmentation of Slc26a4+/+ mice did not compromise F actin localization.
T9612 24400-24407 JJ denotes Minimal
T9613 24408-24420 NN denotes pigmentation
T9615 24421-24423 IN denotes of
T9616 24424-24431 NN denotes Slc26a4
T9618 24431-24432 SYM denotes +
T9619 24432-24433 HYPH denotes /
T9620 24433-24434 SYM denotes +
T9617 24435-24439 NNS denotes mice
T9621 24440-24443 VBD denotes did
T9622 24444-24447 RB denotes not
T9614 24448-24458 VB denotes compromise
T9623 24459-24460 NN denotes F
T9624 24461-24466 NN denotes actin
T9625 24467-24479 NN denotes localization
T9626 24479-24480 . denotes .
T9627 24480-24622 sentence denotes Whole-mounts of stria vascularis from Slc26a4-/- mice were viewed either from the basal cell side (e-f) or from the marginal cell side (h-i).
T9628 24481-24486 JJ denotes Whole
T9630 24486-24487 HYPH denotes -
T9629 24487-24493 NNS denotes mounts
T9632 24494-24496 IN denotes of
T9633 24497-24502 NN denotes stria
T9634 24503-24513 NN denotes vascularis
T9635 24514-24518 IN denotes from
T9636 24519-24526 NN denotes Slc26a4
T9638 24526-24527 SYM denotes -
T9639 24527-24528 HYPH denotes /
T9640 24528-24529 SYM denotes -
T9637 24530-24534 NNS denotes mice
T9641 24535-24539 VBD denotes were
T9631 24540-24546 VBN denotes viewed
T9642 24547-24553 CC denotes either
T9643 24554-24558 IN denotes from
T9644 24559-24562 DT denotes the
T9646 24563-24568 JJ denotes basal
T9647 24569-24573 NN denotes cell
T9645 24574-24578 NN denotes side
T9648 24579-24580 -LRB- denotes (
T9649 24580-24581 NN denotes e
T9650 24581-24582 SYM denotes -
T9651 24582-24583 NN denotes f
T9652 24583-24584 -RRB- denotes )
T9653 24585-24587 CC denotes or
T9654 24588-24592 IN denotes from
T9655 24593-24596 DT denotes the
T9657 24597-24605 JJ denotes marginal
T9658 24606-24610 NN denotes cell
T9656 24611-24615 NN denotes side
T9659 24616-24617 -LRB- denotes (
T9660 24617-24618 NN denotes h
T9661 24618-24619 SYM denotes -
T9662 24619-24620 NN denotes i
T9663 24620-24621 -RRB- denotes )
T9664 24621-24622 . denotes .
T9665 24622-24707 sentence denotes Bright field images verify that the same area was viewed from either side (d and g).
T9666 24623-24629 JJ denotes Bright
T9667 24630-24635 NN denotes field
T9668 24636-24642 NNS denotes images
T9669 24643-24649 VBP denotes verify
T9670 24650-24654 IN denotes that
T9672 24655-24658 DT denotes the
T9674 24659-24663 JJ denotes same
T9673 24664-24668 NN denotes area
T9675 24669-24672 VBD denotes was
T9671 24673-24679 VBN denotes viewed
T9676 24680-24684 IN denotes from
T9677 24685-24691 CC denotes either
T9678 24692-24696 NN denotes side
T9679 24697-24698 -LRB- denotes (
T9680 24698-24699 NN denotes d
T9681 24700-24703 CC denotes and
T9682 24704-24705 NN denotes g
T9683 24705-24706 -RRB- denotes )
T9684 24706-24707 . denotes .
T9685 24707-24830 sentence denotes Focus was varied to either visualize the marginal cell barrier (SMC, b,e and h) or the basal cell barrier (BC, c,f and i).
T9686 24708-24713 NN denotes Focus
T9688 24714-24717 VBD denotes was
T9687 24718-24724 VBN denotes varied
T9689 24725-24727 TO denotes to
T9691 24728-24734 CC denotes either
T9690 24735-24744 VB denotes visualize
T9692 24745-24748 DT denotes the
T9694 24749-24757 JJ denotes marginal
T9695 24758-24762 NN denotes cell
T9693 24763-24770 NN denotes barrier
T9696 24771-24772 -LRB- denotes (
T9698 24772-24775 NN denotes SMC
T9699 24775-24777 , denotes ,
T9697 24777-24778 NN denotes b
T9700 24778-24779 , denotes ,
T9701 24779-24780 NN denotes e
T9702 24781-24784 CC denotes and
T9703 24785-24786 NN denotes h
T9704 24786-24787 -RRB- denotes )
T9705 24788-24790 CC denotes or
T9706 24791-24794 DT denotes the
T9708 24795-24800 JJ denotes basal
T9709 24801-24805 NN denotes cell
T9707 24806-24813 NN denotes barrier
T9710 24814-24815 -LRB- denotes (
T9712 24815-24817 NN denotes BC
T9713 24817-24819 , denotes ,
T9711 24819-24820 NN denotes c
T9714 24820-24821 , denotes ,
T9715 24821-24822 NN denotes f
T9716 24823-24826 CC denotes and
T9717 24827-24828 NN denotes i
T9718 24828-24829 -RRB- denotes )
T9719 24829-24830 . denotes .
T9720 24830-24912 sentence denotes Both the basal cell (f) and the marginal cell (h) barriers appeared to be intact.
T9721 24831-24835 CC denotes Both
T9723 24836-24839 DT denotes the
T9724 24840-24845 JJ denotes basal
T9722 24846-24850 NN denotes cell
T9726 24851-24852 -LRB- denotes (
T9727 24852-24853 NN denotes f
T9728 24853-24854 -RRB- denotes )
T9729 24855-24858 CC denotes and
T9730 24859-24862 DT denotes the
T9732 24863-24871 JJ denotes marginal
T9731 24872-24876 NN denotes cell
T9734 24877-24878 -LRB- denotes (
T9735 24878-24879 NN denotes h
T9736 24879-24880 -RRB- denotes )
T9733 24881-24889 NNS denotes barriers
T9725 24890-24898 VBD denotes appeared
T9737 24899-24901 TO denotes to
T9738 24902-24904 VB denotes be
T9739 24905-24911 JJ denotes intact
T9740 24911-24912 . denotes .
T9741 24912-25052 sentence denotes Blockage of the laser by pigmentation produces the untrue impression of 'holes' in the marginal cell barrier (e) or basal cell barrier (i).
T9742 24913-24921 NN denotes Blockage
T9744 24922-24924 IN denotes of
T9745 24925-24928 DT denotes the
T9746 24929-24934 NN denotes laser
T9747 24935-24937 IN denotes by
T9748 24938-24950 NN denotes pigmentation
T9743 24951-24959 VBZ denotes produces
T9749 24960-24963 DT denotes the
T9751 24964-24970 JJ denotes untrue
T9750 24971-24981 NN denotes impression
T9752 24982-24984 IN denotes of
T9753 24985-24986 `` denotes '
T9754 24986-24991 NNS denotes holes
T9755 24991-24992 '' denotes '
T9756 24993-24995 IN denotes in
T9757 24996-24999 DT denotes the
T9759 25000-25008 JJ denotes marginal
T9760 25009-25013 NN denotes cell
T9758 25014-25021 NN denotes barrier
T9761 25022-25023 -LRB- denotes (
T9762 25023-25024 NN denotes e
T9763 25024-25025 -RRB- denotes )
T9764 25026-25028 CC denotes or
T9765 25029-25034 JJ denotes basal
T9766 25035-25039 NN denotes cell
T9767 25040-25047 NN denotes barrier
T9768 25048-25049 -LRB- denotes (
T9769 25049-25050 NN denotes i
T9770 25050-25051 -RRB- denotes )
T9771 25051-25052 . denotes .
T9772 25052-25137 sentence denotes Comparison of images is aided by marking three significant areas with colored stars.
T9773 25053-25063 NN denotes Comparison
T9775 25064-25066 IN denotes of
T9776 25067-25073 NNS denotes images
T9777 25074-25076 VBZ denotes is
T9774 25077-25082 VBN denotes aided
T9778 25083-25085 IN denotes by
T9779 25086-25093 VBG denotes marking
T9780 25094-25099 CD denotes three
T9782 25100-25111 JJ denotes significant
T9781 25112-25117 NNS denotes areas
T9783 25118-25122 IN denotes with
T9784 25123-25130 JJ denotes colored
T9785 25131-25136 NNS denotes stars
T9786 25136-25137 . denotes .
T9787 25137-25151 sentence denotes Bars = 10 μm.
T9788 25138-25142 NNS denotes Bars
T9790 25143-25144 SYM denotes =
T9791 25145-25147 CD denotes 10
T9789 25148-25150 NNS denotes μm
T9792 25150-25151 . denotes .
T6484 25152-25153 NN denotes P
T6482 25152-25159 sentence denotes Pendrin
T6483 25153-25154 VBN denotes e
T6485 25154-25157 JJ denotes ndr
T6487 25157-25158 JJ denotes i
T6486 25158-25159 NN denotes n
T6490 25159-25160 SYM denotes -
T6488 25159-25161 sentence denotes -e
T6489 25160-25161 NNS denotes e
T6492 25161-25162 VBN denotes x
T6491 25161-25166 sentence denotes xpres
T6493 25162-25163 NN denotes p
T6494 25163-25166 NNS denotes res
T6496 25166-25167 NN denotes s
T6495 25166-25169 sentence denotes sin
T6497 25167-25168 VBN denotes i
T6498 25168-25169 JJ denotes n
T6500 25169-25170 JJ denotes g
T6499 25169-25172 sentence denotes g s
T6501 25171-25172 JJ denotes s
T6503 25172-25173 VBZ denotes u
T6502 25172-25180 sentence denotes urface e
T6504 25173-25174 JJ denotes r
T6505 25174-25175 IN denotes f
T6506 25175-25176 DT denotes a
T6508 25176-25177 JJ denotes c
T6509 25177-25178 NN denotes e
T6507 25179-25180 NN denotes e
T6511 25180-25182 NN denotes pi
T6510 25180-25184 sentence denotes pith
T6512 25182-25183 JJ denotes t
T6513 25183-25184 IN denotes h
T6515 25184-25185 NN denotes e
T6514 25184-25185 sentence denotes e
T6517 25185-25187 NN denotes li
T6516 25185-25199 sentence denotes lial cells in
T6518 25187-25189 JJ denotes al
T6520 25190-25194 NN denotes cell
T6519 25194-25195 NNS denotes s
T6521 25196-25198 IN denotes in
T6523 25199-25200 JJ denotes t
T6522 25199-25204 sentence denotes the s
T6524 25200-25202 JJ denotes he
T6525 25203-25204 NN denotes s
T6527 25204-25206 NN denotes pi
T6526 25204-25206 sentence denotes pi
T6529 25206-25209 JJ denotes ral
T6528 25206-25210 sentence denotes ral
T6531 25210-25211 NN denotes p
T6530 25210-25216 sentence denotes promin
T6532 25211-25215 VB denotes romi
T6533 25215-25216 NN denotes n
T6535 25216-25217 JJ denotes e
T6534 25216-25225 sentence denotes ence regi
T6536 25217-25218 NNS denotes n
T6537 25218-25219 NN denotes c
T6538 25219-25220 NNS denotes e
T6539 25221-25223 VBD denotes re
T6540 25223-25225 JJ denotes gi
T6542 25225-25226 IN denotes o
T6541 25225-25228 sentence denotes on
T6543 25226-25227 CC denotes n
T6546 25228-25229 VBD denotes a
T6544 25228-25236 sentence denotes are loca
T6545 25229-25231 VBN denotes re
T6547 25232-25233 VB denotes l
T6548 25233-25234 CC denotes o
T6550 25234-25235 NN denotes c
T6549 25235-25236 NN denotes a
T6552 25236-25237 CC denotes t
T6551 25236-25245 sentence denotes ted in an
T6553 25237-25238 DT denotes e
T6554 25238-25239 CC denotes d
T6555 25240-25242 JJ denotes in
T6556 25243-25244 NNS denotes a
T6557 25244-25245 JJ denotes n
T6558 25245-25251 sentence denotes area
T6559 25246-25247 NN denotes a
T6560 25247-25248 NN denotes r
T6561 25248-25250 NN denotes ea
T6563 25251-25253 IN denotes wh
T6562 25251-25255 sentence denotes wher
T6564 25253-25254 JJ denotes e
T6565 25254-25255 NNS denotes r
T6567 25255-25256 NN denotes e
T6566 25255-25382 sentence denotes e basal cells, which are interconnected by tight junctions, form additional tight junctions with surface epithelial cells [29].
T6569 25257-25262 JJ denotes basal
T6570 25263-25268 NNS denotes cells
T6571 25268-25270 , denotes ,
T6572 25270-25275 WDT denotes which
T6573 25276-25279 VBP denotes are
T6574 25280-25294 VBN denotes interconnected
T6575 25295-25297 IN denotes by
T6576 25298-25303 JJ denotes tight
T6577 25304-25313 NNS denotes junctions
T6578 25313-25315 , denotes ,
T6568 25315-25319 VBP denotes form
T6579 25320-25330 JJ denotes additional
T6581 25331-25336 JJ denotes tight
T6580 25337-25346 NNS denotes junctions
T6582 25347-25351 IN denotes with
T6583 25352-25359 NN denotes surface
T6585 25360-25370 JJ denotes epithelial
T6584 25371-25376 NNS denotes cells
T6586 25377-25378 -LRB- denotes [
T6587 25378-25380 CD denotes 29
T6588 25380-25381 -RRB- denotes ]
T6589 25381-25382 . denotes .
T6590 25382-25499 sentence denotes A discontinuity of this complex junction in Slc26a4-/- mice would explain the absence of the endocochlear potential.
T6591 25383-25384 DT denotes A
T6592 25385-25398 NN denotes discontinuity
T6594 25399-25401 IN denotes of
T6595 25402-25406 DT denotes this
T6597 25407-25414 JJ denotes complex
T6596 25415-25423 NN denotes junction
T6598 25424-25426 IN denotes in
T6599 25427-25434 NN denotes Slc26a4
T6601 25434-25435 SYM denotes -
T6602 25435-25436 HYPH denotes /
T6603 25436-25437 SYM denotes -
T6600 25438-25442 NNS denotes mice
T6604 25443-25448 MD denotes would
T6593 25449-25456 VB denotes explain
T6605 25457-25460 DT denotes the
T6606 25461-25468 NN denotes absence
T6607 25469-25471 IN denotes of
T6608 25472-25475 DT denotes the
T6610 25476-25488 JJ denotes endocochlear
T6609 25489-25498 NN denotes potential
T6611 25498-25499 . denotes .
T6612 25499-25676 sentence denotes To evaluate the presence of this connection, we determined by confocal immunocytochemistry the expression of ZO-1 and of F-actin, which associate with tight junction complexes.
T6613 25500-25502 TO denotes To
T6614 25503-25511 VB denotes evaluate
T6616 25512-25515 DT denotes the
T6617 25516-25524 NN denotes presence
T6618 25525-25527 IN denotes of
T6619 25528-25532 DT denotes this
T6620 25533-25543 NN denotes connection
T6621 25543-25545 , denotes ,
T6622 25545-25547 PRP denotes we
T6615 25548-25558 VBD denotes determined
T6623 25559-25561 IN denotes by
T6624 25562-25570 JJ denotes confocal
T6625 25571-25590 NN denotes immunocytochemistry
T6626 25591-25594 DT denotes the
T6627 25595-25605 NN denotes expression
T6628 25606-25608 IN denotes of
T6629 25609-25611 NN denotes ZO
T6630 25611-25612 HYPH denotes -
T6631 25612-25613 CD denotes 1
T6632 25614-25617 CC denotes and
T6633 25618-25620 IN denotes of
T6634 25621-25622 NN denotes F
T6636 25622-25623 HYPH denotes -
T6635 25623-25628 NN denotes actin
T6637 25628-25630 , denotes ,
T6638 25630-25635 WDT denotes which
T6639 25636-25645 VBP denotes associate
T6640 25646-25650 IN denotes with
T6641 25651-25656 JJ denotes tight
T6643 25657-25665 NN denotes junction
T6642 25666-25675 NNS denotes complexes
T6644 25675-25676 . denotes .
T6645 25676-25885 sentence denotes ZO-1 and F-actin expression revealed a continuous layer of basal cells, including a junction of basal cells with surface epithelial cells in Slc26a4-/- mice, as observed in normal mice (Fig. 1c,1d,1e,1f,6,7).
T6646 25677-25679 NN denotes ZO
T6648 25679-25680 HYPH denotes -
T6649 25680-25681 CD denotes 1
T6650 25682-25685 CC denotes and
T6651 25686-25687 NN denotes F
T6653 25687-25688 HYPH denotes -
T6652 25688-25693 NN denotes actin
T6647 25694-25704 NN denotes expression
T6654 25705-25713 VBD denotes revealed
T6655 25714-25715 DT denotes a
T6657 25716-25726 JJ denotes continuous
T6656 25727-25732 NN denotes layer
T6658 25733-25735 IN denotes of
T6659 25736-25741 JJ denotes basal
T6660 25742-25747 NNS denotes cells
T6661 25747-25749 , denotes ,
T6662 25749-25758 VBG denotes including
T6663 25759-25760 DT denotes a
T6664 25761-25769 NN denotes junction
T6665 25770-25772 IN denotes of
T6666 25773-25778 JJ denotes basal
T6667 25779-25784 NNS denotes cells
T6668 25785-25789 IN denotes with
T6669 25790-25797 NN denotes surface
T6671 25798-25808 JJ denotes epithelial
T6670 25809-25814 NNS denotes cells
T6672 25815-25817 IN denotes in
T6673 25818-25825 NN denotes Slc26a4
T6675 25825-25826 SYM denotes -
T6676 25826-25827 HYPH denotes /
T6677 25827-25828 SYM denotes -
T6674 25829-25833 NNS denotes mice
T6678 25833-25835 , denotes ,
T6679 25835-25837 IN denotes as
T6680 25838-25846 VBN denotes observed
T6681 25847-25849 IN denotes in
T6682 25850-25856 JJ denotes normal
T6683 25857-25861 NNS denotes mice
T6684 25862-25863 -LRB- denotes (
T6686 25863-25867 NN denotes Fig.
T6688 25868-25870 CD denotes 1c
T6689 25870-25871 , denotes ,
T6690 25871-25873 CD denotes 1d
T6691 25873-25874 , denotes ,
T6692 25874-25876 CD denotes 1e
T6693 25876-25877 , denotes ,
T6687 25877-25879 CD denotes 1f
T6694 25879-25880 , denotes ,
T6695 25880-25881 CD denotes 6
T6696 25881-25882 , denotes ,
T6685 25882-25883 CD denotes 7
T6697 25883-25884 -RRB- denotes )
T6698 25884-25885 . denotes .
T6699 25885-26029 sentence denotes These observations make it unlikely that the primary cause of the loss of the endocochlear potential is a compromise in the basal cell barrier.
T6700 25886-25891 DT denotes These
T6701 25892-25904 NNS denotes observations
T6702 25905-25909 VBP denotes make
T6703 25910-25912 PRP denotes it
T6704 25913-25921 JJ denotes unlikely
T6705 25922-25926 IN denotes that
T6707 25927-25930 DT denotes the
T6709 25931-25938 JJ denotes primary
T6708 25939-25944 NN denotes cause
T6710 25945-25947 IN denotes of
T6711 25948-25951 DT denotes the
T6712 25952-25956 NN denotes loss
T6713 25957-25959 IN denotes of
T6714 25960-25963 DT denotes the
T6716 25964-25976 JJ denotes endocochlear
T6715 25977-25986 NN denotes potential
T6706 25987-25989 VBZ denotes is
T6717 25990-25991 DT denotes a
T6718 25992-26002 NN denotes compromise
T6719 26003-26005 IN denotes in
T6720 26006-26009 DT denotes the
T6722 26010-26015 JJ denotes basal
T6723 26016-26020 NN denotes cell
T6721 26021-26028 NN denotes barrier
T6724 26028-26029 . denotes .
T6725 26029-26233 sentence denotes The observation that endolymphatic and perilymphatic K+ concentrations were normal in Slc26a4-/- mice suggests that stria vascularis was able to secrete K+ in spite of the apparent signs of degeneration.
T6726 26030-26033 DT denotes The
T6727 26034-26045 NN denotes observation
T6729 26046-26050 IN denotes that
T6731 26051-26064 JJ denotes endolymphatic
T6733 26065-26068 CC denotes and
T6734 26069-26082 JJ denotes perilymphatic
T6735 26083-26084 NN denotes K
T6736 26084-26085 SYM denotes +
T6732 26086-26100 NNS denotes concentrations
T6730 26101-26105 VBD denotes were
T6737 26106-26112 JJ denotes normal
T6738 26113-26115 IN denotes in
T6739 26116-26123 NN denotes Slc26a4
T6741 26123-26124 SYM denotes -
T6742 26124-26125 HYPH denotes /
T6743 26125-26126 SYM denotes -
T6740 26127-26131 NNS denotes mice
T6728 26132-26140 VBZ denotes suggests
T6744 26141-26145 IN denotes that
T6746 26146-26151 NN denotes stria
T6747 26152-26162 NN denotes vascularis
T6745 26163-26166 VBD denotes was
T6748 26167-26171 JJ denotes able
T6749 26172-26174 TO denotes to
T6750 26175-26182 VB denotes secrete
T6751 26183-26184 NN denotes K
T6752 26184-26185 SYM denotes +
T6753 26186-26188 IN denotes in
T6754 26189-26194 NN denotes spite
T6755 26195-26197 IN denotes of
T6756 26198-26201 DT denotes the
T6758 26202-26210 JJ denotes apparent
T6757 26211-26216 NNS denotes signs
T6759 26217-26219 IN denotes of
T6760 26220-26232 NN denotes degeneration
T6761 26232-26233 . denotes .
T6762 26233-26482 sentence denotes The rate of K+ secretion necessary to maintain endolymphatic K+ concentration, however, may be less than necessary in Slc26a4+/+ mice given the reduced numbers of sensory hair cells, which provide a major pathway for K+ exit from endolymph [30,31].
T6763 26234-26237 DT denotes The
T6764 26238-26242 NN denotes rate
T6766 26243-26245 IN denotes of
T6767 26246-26247 NN denotes K
T6769 26247-26248 SYM denotes +
T6768 26249-26258 NN denotes secretion
T6770 26259-26268 JJ denotes necessary
T6771 26269-26271 TO denotes to
T6772 26272-26280 VB denotes maintain
T6773 26281-26294 JJ denotes endolymphatic
T6775 26295-26296 NN denotes K
T6776 26296-26297 SYM denotes +
T6774 26298-26311 NN denotes concentration
T6777 26311-26313 , denotes ,
T6778 26313-26320 RB denotes however
T6779 26320-26322 , denotes ,
T6780 26322-26325 MD denotes may
T6765 26326-26328 VB denotes be
T6781 26329-26333 JJR denotes less
T6782 26334-26338 IN denotes than
T6783 26339-26348 JJ denotes necessary
T6784 26349-26351 IN denotes in
T6785 26352-26359 NN denotes Slc26a4
T6787 26359-26360 SYM denotes +
T6788 26360-26361 HYPH denotes /
T6789 26361-26362 SYM denotes +
T6786 26363-26367 NNS denotes mice
T6790 26368-26373 VBN denotes given
T6791 26374-26377 DT denotes the
T6793 26378-26385 JJ denotes reduced
T6792 26386-26393 NNS denotes numbers
T6794 26394-26396 IN denotes of
T6795 26397-26404 JJ denotes sensory
T6797 26405-26409 NN denotes hair
T6796 26410-26415 NNS denotes cells
T6798 26415-26417 , denotes ,
T6799 26417-26422 WDT denotes which
T6800 26423-26430 VBP denotes provide
T6801 26431-26432 DT denotes a
T6803 26433-26438 JJ denotes major
T6802 26439-26446 NN denotes pathway
T6804 26447-26450 IN denotes for
T6805 26451-26452 NN denotes K
T6807 26452-26453 SYM denotes +
T6806 26454-26458 NN denotes exit
T6808 26459-26463 IN denotes from
T6809 26464-26473 NN denotes endolymph
T6810 26474-26475 -LRB- denotes [
T6812 26475-26477 CD denotes 30
T6813 26477-26478 , denotes ,
T6811 26478-26480 CD denotes 31
T6814 26480-26481 -RRB- denotes ]
T6815 26481-26482 . denotes .
T6816 26482-26896 sentence denotes In order to substantiate the view that stria vascularis in Slc26a4-/- secretes K+, we measured the magnitude of the bumetanide-sensitive current exiting stria vascularis across the apical membrane of strial marginal cells and determined the expression of the proteins KCNQ1, KCNE1 and SLC12A2, which are essential for K+ secretion [20,32,33], and of GJB2 (Cx26), which is thought to contribute to K+ cycling [23].
T6817 26483-26485 IN denotes In
T6819 26486-26491 NN denotes order
T6820 26492-26494 TO denotes to
T6821 26495-26507 VB denotes substantiate
T6822 26508-26511 DT denotes the
T6823 26512-26516 NN denotes view
T6824 26517-26521 IN denotes that
T6826 26522-26527 NN denotes stria
T6827 26528-26538 NN denotes vascularis
T6828 26539-26541 IN denotes in
T6829 26542-26549 NN denotes Slc26a4
T6830 26549-26550 SYM denotes -
T6831 26550-26551 HYPH denotes /
T6832 26551-26552 SYM denotes -
T6825 26553-26561 VBZ denotes secretes
T6833 26562-26563 NN denotes K
T6834 26563-26564 SYM denotes +
T6835 26564-26566 , denotes ,
T6836 26566-26568 PRP denotes we
T6818 26569-26577 VBD denotes measured
T6837 26578-26581 DT denotes the
T6838 26582-26591 NN denotes magnitude
T6839 26592-26594 IN denotes of
T6840 26595-26598 DT denotes the
T6842 26599-26609 NN denotes bumetanide
T6844 26609-26610 HYPH denotes -
T6843 26610-26619 JJ denotes sensitive
T6841 26620-26627 NN denotes current
T6845 26628-26635 VBG denotes exiting
T6846 26636-26641 NN denotes stria
T6847 26642-26652 NN denotes vascularis
T6848 26653-26659 IN denotes across
T6849 26660-26663 DT denotes the
T6851 26664-26670 JJ denotes apical
T6850 26671-26679 NN denotes membrane
T6852 26680-26682 IN denotes of
T6853 26683-26689 JJ denotes strial
T6855 26690-26698 JJ denotes marginal
T6854 26699-26704 NNS denotes cells
T6856 26705-26708 CC denotes and
T6857 26709-26719 VBD denotes determined
T6858 26720-26723 DT denotes the
T6859 26724-26734 NN denotes expression
T6860 26735-26737 IN denotes of
T6861 26738-26741 DT denotes the
T6862 26742-26750 NN denotes proteins
T6863 26751-26756 NN denotes KCNQ1
T6864 26756-26758 , denotes ,
T6865 26758-26763 NN denotes KCNE1
T6866 26764-26767 CC denotes and
T6867 26768-26775 NN denotes SLC12A2
T6868 26775-26777 , denotes ,
T6869 26777-26782 WDT denotes which
T6870 26783-26786 VBP denotes are
T6871 26787-26796 JJ denotes essential
T6872 26797-26800 IN denotes for
T6873 26801-26802 NN denotes K
T6875 26802-26803 SYM denotes +
T6874 26804-26813 NN denotes secretion
T6876 26814-26815 -LRB- denotes [
T6878 26815-26817 CD denotes 20
T6879 26817-26818 , denotes ,
T6880 26818-26820 CD denotes 32
T6881 26820-26821 , denotes ,
T6877 26821-26823 CD denotes 33
T6882 26823-26824 -RRB- denotes ]
T6883 26824-26826 , denotes ,
T6884 26826-26829 CC denotes and
T6885 26830-26832 IN denotes of
T6886 26833-26837 NN denotes GJB2
T6888 26838-26839 -LRB- denotes (
T6887 26839-26843 NN denotes Cx26
T6889 26843-26844 -RRB- denotes )
T6890 26844-26846 , denotes ,
T6891 26846-26851 WDT denotes which
T6893 26852-26854 VBZ denotes is
T6892 26855-26862 VBN denotes thought
T6894 26863-26865 TO denotes to
T6895 26866-26876 VB denotes contribute
T6896 26877-26879 IN denotes to
T6897 26880-26881 NN denotes K
T6899 26881-26882 SYM denotes +
T6898 26883-26890 NN denotes cycling
T6900 26891-26892 -LRB- denotes [
T6901 26892-26894 CD denotes 23
T6902 26894-26895 -RRB- denotes ]
T6903 26895-26896 . denotes .
T6904 26896-27030 sentence denotes K+ secretion is known to be sensitive to 10-5 M bumetanide, a loop-diuretic that inhibits the Na+/2Cl-/K+ cotransporter SLC12A2 [26].
T6905 26897-26898 NN denotes K
T6907 26898-26899 SYM denotes +
T6906 26900-26909 NN denotes secretion
T6909 26910-26912 VBZ denotes is
T6908 26913-26918 VBN denotes known
T6910 26919-26921 TO denotes to
T6911 26922-26924 VB denotes be
T6912 26925-26934 JJ denotes sensitive
T6913 26935-26937 IN denotes to
T6914 26938-26940 CD denotes 10
T6916 26940-26941 HYPH denotes -
T6915 26941-26942 CD denotes 5
T6917 26943-26944 NN denotes M
T6918 26945-26955 NN denotes bumetanide
T6919 26955-26957 , denotes ,
T6920 26957-26958 DT denotes a
T6922 26959-26963 NN denotes loop
T6923 26963-26964 HYPH denotes -
T6921 26964-26972 NN denotes diuretic
T6924 26973-26977 WDT denotes that
T6925 26978-26986 VBZ denotes inhibits
T6926 26987-26990 DT denotes the
T6928 26991-26993 NN denotes Na
T6929 26993-26994 SYM denotes +
T6930 26994-26995 HYPH denotes /
T6931 26995-26998 NN denotes 2Cl
T6932 26998-26999 SYM denotes -
T6933 26999-27000 HYPH denotes /
T6934 27000-27001 NN denotes K
T6935 27001-27002 SYM denotes +
T6927 27003-27016 NN denotes cotransporter
T6936 27017-27024 NN denotes SLC12A2
T6937 27025-27026 -LRB- denotes [
T6938 27026-27028 CD denotes 26
T6939 27028-27029 -RRB- denotes ]
T6940 27029-27030 . denotes .
T6941 27030-27245 sentence denotes Bumetanide-sensitive currents were found in both Slc26a4+/+ and Slc26a4-/- mice although the magnitude of the current was significantly smaller in Slc26a4-/- mice (22 ± 6 μA/cm2, n = 4 versus 14 ± 2 μA/cm2, n = 4).
T6942 27031-27041 NN denotes Bumetanide
T6944 27041-27042 HYPH denotes -
T6943 27042-27051 JJ denotes sensitive
T6945 27052-27060 NNS denotes currents
T6947 27061-27065 VBD denotes were
T6946 27066-27071 VBN denotes found
T6948 27072-27074 IN denotes in
T6949 27075-27079 CC denotes both
T6950 27080-27087 NN denotes Slc26a4
T6952 27087-27088 SYM denotes +
T6953 27088-27089 HYPH denotes /
T6954 27089-27090 SYM denotes +
T6955 27091-27094 CC denotes and
T6956 27095-27102 NN denotes Slc26a4
T6957 27102-27103 SYM denotes -
T6958 27103-27104 HYPH denotes /
T6959 27104-27105 SYM denotes -
T6951 27106-27110 NNS denotes mice
T6960 27111-27119 IN denotes although
T6962 27120-27123 DT denotes the
T6963 27124-27133 NN denotes magnitude
T6964 27134-27136 IN denotes of
T6965 27137-27140 DT denotes the
T6966 27141-27148 NN denotes current
T6961 27149-27152 VBD denotes was
T6967 27153-27166 RB denotes significantly
T6968 27167-27174 JJR denotes smaller
T6969 27175-27177 IN denotes in
T6970 27178-27185 NN denotes Slc26a4
T6972 27185-27186 SYM denotes -
T6973 27186-27187 HYPH denotes /
T6974 27187-27188 SYM denotes -
T6971 27189-27193 NNS denotes mice
T6975 27194-27195 -LRB- denotes (
T6977 27195-27197 CD denotes 22
T6979 27198-27199 SYM denotes ±
T6978 27200-27201 CD denotes 6
T6980 27202-27204 NN denotes μA
T6981 27204-27205 SYM denotes /
T6982 27205-27208 NN denotes cm2
T6983 27208-27210 , denotes ,
T6984 27210-27211 NN denotes n
T6985 27212-27213 SYM denotes =
T6976 27214-27215 CD denotes 4
T6986 27216-27222 CC denotes versus
T6987 27223-27225 CD denotes 14
T6989 27226-27227 SYM denotes ±
T6988 27228-27229 CD denotes 2
T6990 27230-27232 NNS denotes μA
T6992 27232-27233 SYM denotes /
T6993 27233-27236 NN denotes cm2
T6994 27236-27238 , denotes ,
T6995 27238-27239 NN denotes n
T6996 27240-27241 SYM denotes =
T6991 27242-27243 CD denotes 4
T6997 27243-27244 -RRB- denotes )
T6998 27244-27245 . denotes .
T6999 27245-27615 sentence denotes KCNQ1 and KCNE1, subunits of the secretory K+ channel, were co-localized in the apical membrane of strial marginal cells; the Na+/2Cl-/K+ cotransporter SLC12A2, which is located in the basolateral membrane of strial marginal cells, was found in stria vascularis; and the gap junction protein GJB2 was found in spiral ligament of Slc26a4+/+ and Slc26a4-/- mice (Fig. 8).
T7000 27246-27251 NN denotes KCNQ1
T7002 27252-27255 CC denotes and
T7003 27256-27261 NN denotes KCNE1
T7004 27261-27263 , denotes ,
T7005 27263-27271 NNS denotes subunits
T7006 27272-27274 IN denotes of
T7007 27275-27278 DT denotes the
T7009 27279-27288 JJ denotes secretory
T7010 27289-27290 NN denotes K
T7011 27290-27291 SYM denotes +
T7008 27292-27299 NN denotes channel
T7012 27299-27301 , denotes ,
T7013 27301-27305 VBD denotes were
T7001 27306-27318 VBN denotes co-localized
T7014 27319-27321 IN denotes in
T7015 27322-27325 DT denotes the
T7017 27326-27332 JJ denotes apical
T7016 27333-27341 NN denotes membrane
T7018 27342-27344 IN denotes of
T7019 27345-27351 JJ denotes strial
T7020 27352-27360 JJ denotes marginal
T7021 27361-27366 NNS denotes cells
T7022 27366-27367 : denotes ;
T7023 27368-27371 DT denotes the
T7025 27372-27374 NN denotes Na
T7026 27374-27375 SYM denotes +
T7027 27375-27376 HYPH denotes /
T7028 27376-27379 NN denotes 2Cl
T7029 27379-27380 SYM denotes -
T7030 27380-27381 HYPH denotes /
T7031 27381-27382 NN denotes K
T7032 27382-27383 SYM denotes +
T7024 27384-27397 NN denotes cotransporter
T7034 27398-27405 NN denotes SLC12A2
T7035 27405-27407 , denotes ,
T7036 27407-27412 WDT denotes which
T7038 27413-27415 VBZ denotes is
T7037 27416-27423 VBN denotes located
T7039 27424-27426 IN denotes in
T7040 27427-27430 DT denotes the
T7042 27431-27442 JJ denotes basolateral
T7041 27443-27451 NN denotes membrane
T7043 27452-27454 IN denotes of
T7044 27455-27461 JJ denotes strial
T7046 27462-27470 JJ denotes marginal
T7045 27471-27476 NNS denotes cells
T7047 27476-27478 , denotes ,
T7048 27478-27481 VBD denotes was
T7033 27482-27487 VBN denotes found
T7049 27488-27490 IN denotes in
T7050 27491-27496 NN denotes stria
T7051 27497-27507 NN denotes vascularis
T7052 27507-27508 : denotes ;
T7053 27509-27512 CC denotes and
T7054 27513-27516 DT denotes the
T7056 27517-27520 NN denotes gap
T7057 27521-27529 NN denotes junction
T7055 27530-27537 NN denotes protein
T7059 27538-27542 NN denotes GJB2
T7060 27543-27546 VBD denotes was
T7058 27547-27552 VBN denotes found
T7061 27553-27555 IN denotes in
T7062 27556-27562 JJ denotes spiral
T7063 27563-27571 NN denotes ligament
T7064 27572-27574 IN denotes of
T7065 27575-27582 NN denotes Slc26a4
T7067 27582-27583 SYM denotes +
T7068 27583-27584 HYPH denotes /
T7069 27584-27585 SYM denotes +
T7070 27586-27589 CC denotes and
T7071 27590-27597 NN denotes Slc26a4
T7072 27597-27598 SYM denotes -
T7073 27598-27599 HYPH denotes /
T7074 27599-27600 SYM denotes -
T7066 27601-27605 NNS denotes mice
T7075 27606-27607 -LRB- denotes (
T7076 27607-27611 NN denotes Fig.
T7077 27612-27613 CD denotes 8
T7078 27613-27614 -RRB- denotes )
T7079 27614-27615 . denotes .
T7080 27615-27713 sentence denotes Co-localization of KCNQ1 and KCNE1 proteins was also observed in vestibular dark cells (Fig. 1i).
T7081 27616-27631 NN denotes Co-localization
T7083 27632-27634 IN denotes of
T7084 27635-27640 NN denotes KCNQ1
T7086 27641-27644 CC denotes and
T7087 27645-27650 NN denotes KCNE1
T7085 27651-27659 NN denotes proteins
T7088 27660-27663 VBD denotes was
T7089 27664-27668 RB denotes also
T7082 27669-27677 VBN denotes observed
T7090 27678-27680 IN denotes in
T7091 27681-27691 JJ denotes vestibular
T7093 27692-27696 JJ denotes dark
T7092 27697-27702 NNS denotes cells
T7094 27703-27704 -LRB- denotes (
T7095 27704-27708 NN denotes Fig.
T7096 27709-27711 CD denotes 1i
T7097 27711-27712 -RRB- denotes )
T7098 27712-27713 . denotes .
T7099 27713-27846 sentence denotes Expression of KCNQ1 protein in stria vascularis of Slc26a4-/- mice is consistent with the finding of KCNQ1 mRNA expression (Fig. 3).
T7100 27714-27724 NN denotes Expression
T7102 27725-27727 IN denotes of
T7103 27728-27733 NN denotes KCNQ1
T7104 27734-27741 NN denotes protein
T7105 27742-27744 IN denotes in
T7106 27745-27750 NN denotes stria
T7107 27751-27761 NN denotes vascularis
T7108 27762-27764 IN denotes of
T7109 27765-27772 NN denotes Slc26a4
T7111 27772-27773 SYM denotes -
T7112 27773-27774 HYPH denotes /
T7113 27774-27775 SYM denotes -
T7110 27776-27780 NNS denotes mice
T7101 27781-27783 VBZ denotes is
T7114 27784-27794 JJ denotes consistent
T7115 27795-27799 IN denotes with
T7116 27800-27803 DT denotes the
T7117 27804-27811 NN denotes finding
T7118 27812-27814 IN denotes of
T7119 27815-27820 NN denotes KCNQ1
T7120 27821-27825 NN denotes mRNA
T7121 27826-27836 NN denotes expression
T7122 27837-27838 -LRB- denotes (
T7123 27838-27842 NN denotes Fig.
T7124 27843-27844 CD denotes 3
T7125 27844-27845 -RRB- denotes )
T7126 27845-27846 . denotes .
T7127 27846-28057 sentence denotes These observations make it unlikely that the primary cause of the loss of the endocochlear potential is a compromise of K+ secretion by strial marginal cells or a compromise of gap junction mediated K+ cycling.
T7128 27847-27852 DT denotes These
T7129 27853-27865 NNS denotes observations
T7130 27866-27870 VBP denotes make
T7131 27871-27873 PRP denotes it
T7132 27874-27882 JJ denotes unlikely
T7133 27883-27887 IN denotes that
T7135 27888-27891 DT denotes the
T7137 27892-27899 JJ denotes primary
T7136 27900-27905 NN denotes cause
T7138 27906-27908 IN denotes of
T7139 27909-27912 DT denotes the
T7140 27913-27917 NN denotes loss
T7141 27918-27920 IN denotes of
T7142 27921-27924 DT denotes the
T7144 27925-27937 JJ denotes endocochlear
T7143 27938-27947 NN denotes potential
T7134 27948-27950 VBZ denotes is
T7145 27951-27952 DT denotes a
T7146 27953-27963 NN denotes compromise
T7147 27964-27966 IN denotes of
T7148 27967-27968 NN denotes K
T7150 27968-27969 SYM denotes +
T7149 27970-27979 NN denotes secretion
T7151 27980-27982 IN denotes by
T7152 27983-27989 JJ denotes strial
T7153 27990-27998 JJ denotes marginal
T7154 27999-28004 NNS denotes cells
T7155 28005-28007 CC denotes or
T7156 28008-28009 DT denotes a
T7157 28010-28020 NN denotes compromise
T7158 28021-28023 IN denotes of
T7159 28024-28027 NN denotes gap
T7160 28028-28036 NN denotes junction
T7161 28037-28045 VBN denotes mediated
T7163 28046-28047 NN denotes K
T7164 28047-28048 SYM denotes +
T7162 28049-28056 NN denotes cycling
T7165 28056-28057 . denotes .
T9850 28068-28075 NN denotes Protein
T9851 28076-28088 NN denotes localization
T9852 28089-28091 IN denotes of
T9853 28092-28097 NN denotes KCNQ1
T9854 28097-28099 , denotes ,
T9855 28099-28104 NN denotes KCNE1
T9856 28104-28106 , denotes ,
T9857 28106-28113 NN denotes SLC12A2
T9858 28114-28117 CC denotes and
T9859 28118-28122 NN denotes GJB2
T9860 28123-28125 IN denotes in
T9861 28126-28129 DT denotes the
T9863 28130-28138 JJ denotes cochlear
T9864 28139-28146 JJ denotes lateral
T9862 28147-28151 NN denotes wall
T9865 28152-28154 IN denotes of
T9866 28155-28162 NN denotes Slc26a4
T9868 28162-28163 SYM denotes +
T9869 28163-28164 HYPH denotes /
T9870 28164-28165 SYM denotes +
T9871 28166-28169 CC denotes and
T9872 28170-28177 NN denotes Slc26a4
T9873 28177-28178 SYM denotes -
T9874 28178-28179 HYPH denotes /
T9875 28179-28180 SYM denotes -
T9867 28181-28185 NNS denotes mice
T9876 28185-28186 . denotes .
T9877 28186-28204 sentence denotes a-f: bars: 10 μm.
T9878 28187-28188 LS denotes a
T9880 28188-28189 SYM denotes -
T9881 28189-28190 LS denotes f
T9882 28190-28192 : denotes :
T9883 28192-28196 NNS denotes bars
T9884 28196-28198 : denotes :
T9885 28198-28200 CD denotes 10
T9879 28201-28203 NNS denotes μm
T9886 28203-28204 . denotes .
T9887 28204-28292 sentence denotes SMC, strial marginal cells; SV, stria vascularis; BC, basal cells; SL, spiral ligament.
T9888 28205-28208 NN denotes SMC
T9889 28208-28210 , denotes ,
T9890 28210-28216 JJ denotes strial
T9892 28217-28225 JJ denotes marginal
T9891 28226-28231 NNS denotes cells
T9893 28231-28232 : denotes ;
T9894 28233-28235 NN denotes SV
T9895 28235-28237 , denotes ,
T9896 28237-28242 NN denotes stria
T9897 28243-28253 NN denotes vascularis
T9898 28253-28254 : denotes ;
T9899 28255-28257 NN denotes BC
T9900 28257-28259 , denotes ,
T9901 28259-28264 JJ denotes basal
T9902 28265-28270 NNS denotes cells
T9903 28270-28271 : denotes ;
T9904 28272-28274 NN denotes SL
T9905 28274-28276 , denotes ,
T9906 28276-28282 JJ denotes spiral
T9907 28283-28291 NN denotes ligament
T9908 28291-28292 . denotes .
T7393 28306-28308 PRP denotes We
T7394 28309-28318 VBD denotes described
T7395 28319-28328 NNS denotes locations
T7396 28329-28331 IN denotes of
T7397 28332-28339 NN denotes pendrin
T7398 28340-28350 NN denotes expression
T7399 28351-28353 IN denotes in
T7400 28354-28357 DT denotes the
T7401 28358-28365 NN denotes cochlea
T7402 28366-28369 CC denotes and
T7403 28370-28380 JJ denotes vestibular
T7404 28381-28390 NN denotes labyrinth
T7405 28391-28394 CC denotes and
T7406 28395-28403 VBD denotes detected
T7407 28404-28410 JJ denotes normal
T7409 28411-28424 JJ denotes endolymphatic
T7410 28425-28426 NN denotes K
T7411 28426-28427 SYM denotes +
T7408 28428-28442 NNS denotes concentrations
T7412 28443-28445 IN denotes in
T7413 28446-28451 NN denotes spite
T7414 28452-28454 IN denotes of
T7415 28455-28457 DT denotes an
T7416 28458-28469 NN denotes enlargement
T7417 28470-28472 IN denotes of
T7418 28473-28477 DT denotes this
T7420 28478-28483 NN denotes fluid
T7419 28484-28495 NN denotes compartment
T7421 28495-28496 . denotes .
T7422 28496-28602 sentence denotes We found that Slc26a4-/- mice lack the endocochlear potential because they do not express KCNJ10 protein.
T7423 28497-28499 PRP denotes We
T7424 28500-28505 VBD denotes found
T7425 28506-28510 IN denotes that
T7427 28511-28518 NN denotes Slc26a4
T7429 28518-28519 SYM denotes -
T7430 28519-28520 HYPH denotes /
T7431 28520-28521 SYM denotes -
T7428 28522-28526 NNS denotes mice
T7426 28527-28531 VBP denotes lack
T7432 28532-28535 DT denotes the
T7434 28536-28548 JJ denotes endocochlear
T7433 28549-28558 NN denotes potential
T7435 28559-28566 IN denotes because
T7437 28567-28571 PRP denotes they
T7438 28572-28574 VBP denotes do
T7439 28575-28578 RB denotes not
T7436 28579-28586 VB denotes express
T7440 28587-28593 NN denotes KCNJ10
T7441 28594-28601 NN denotes protein
T7442 28601-28602 . denotes .
T7443 28602-28717 sentence denotes Intermediate cells protect stria vascularis by converting CO2 to HCO3 - and by detoxifying free radicals (Fig. 9).
T7444 28603-28615 JJ denotes Intermediate
T7445 28616-28621 NNS denotes cells
T7446 28622-28629 VBP denotes protect
T7447 28630-28635 NN denotes stria
T7448 28636-28646 NN denotes vascularis
T7449 28647-28649 IN denotes by
T7450 28650-28660 VBG denotes converting
T7451 28661-28664 NN denotes CO2
T7452 28665-28667 IN denotes to
T7453 28668-28672 NN denotes HCO3
T7454 28673-28674 SYM denotes -
T7455 28675-28678 CC denotes and
T7456 28679-28681 IN denotes by
T7457 28682-28693 VBG denotes detoxifying
T7458 28694-28698 JJ denotes free
T7459 28699-28707 NNS denotes radicals
T7460 28708-28709 -LRB- denotes (
T7461 28709-28713 NN denotes Fig.
T7462 28714-28715 CD denotes 9
T7463 28715-28716 -RRB- denotes )
T7464 28716-28717 . denotes .
T7465 28717-28848 sentence denotes CO2 and free radicals are generated by the large numbers of mitochondria in the metabolically highly active strial marginal cells.
T7466 28718-28721 NN denotes CO2
T7468 28722-28725 CC denotes and
T7469 28726-28730 JJ denotes free
T7470 28731-28739 NNS denotes radicals
T7471 28740-28743 VBP denotes are
T7467 28744-28753 VBN denotes generated
T7472 28754-28756 IN denotes by
T7473 28757-28760 DT denotes the
T7475 28761-28766 JJ denotes large
T7474 28767-28774 NNS denotes numbers
T7476 28775-28777 IN denotes of
T7477 28778-28790 NNS denotes mitochondria
T7478 28791-28793 IN denotes in
T7479 28794-28797 DT denotes the
T7481 28798-28811 RB denotes metabolically
T7483 28812-28818 RB denotes highly
T7482 28819-28825 JJ denotes active
T7484 28826-28832 JJ denotes strial
T7485 28833-28841 JJ denotes marginal
T7480 28842-28847 NNS denotes cells
T7486 28847-28848 . denotes .
T7487 28848-28966 sentence denotes Intermediate cells employ carbonic anhydrase to convert CO2 to HCO3 - [18,19] and catalase to detoxify free radicals.
T7488 28849-28861 JJ denotes Intermediate
T7489 28862-28867 NNS denotes cells
T7490 28868-28874 VBP denotes employ
T7491 28875-28883 JJ denotes carbonic
T7492 28884-28893 NN denotes anhydrase
T7493 28894-28896 TO denotes to
T7494 28897-28904 VB denotes convert
T7495 28905-28908 NN denotes CO2
T7496 28909-28911 IN denotes to
T7497 28912-28916 NN denotes HCO3
T7498 28917-28918 SYM denotes -
T7499 28919-28920 -LRB- denotes [
T7501 28920-28922 CD denotes 18
T7502 28922-28923 , denotes ,
T7500 28923-28925 CD denotes 19
T7503 28925-28926 -RRB- denotes ]
T7504 28927-28930 CC denotes and
T7505 28931-28939 NN denotes catalase
T7506 28940-28942 TO denotes to
T7507 28943-28951 VB denotes detoxify
T7508 28952-28956 JJ denotes free
T7509 28957-28965 NNS denotes radicals
T7510 28965-28966 . denotes .
T7511 28966-29083 sentence denotes To protect themselves from free radical damage, intermediate cells generate glutathione and melanin pigment [34-37].
T7512 28967-28969 TO denotes To
T7513 28970-28977 VB denotes protect
T7515 28978-28988 PRP denotes themselves
T7516 28989-28993 IN denotes from
T7517 28994-28998 JJ denotes free
T7519 28999-29006 NN denotes radical
T7518 29007-29013 NN denotes damage
T7520 29013-29015 , denotes ,
T7521 29015-29027 JJ denotes intermediate
T7522 29028-29033 NNS denotes cells
T7514 29034-29042 VBP denotes generate
T7523 29043-29054 NN denotes glutathione
T7524 29055-29058 CC denotes and
T7525 29059-29066 NN denotes melanin
T7526 29067-29074 NN denotes pigment
T7527 29075-29076 -LRB- denotes [
T7528 29076-29078 CD denotes 34
T7529 29078-29079 SYM denotes -
T7530 29079-29081 CD denotes 37
T7531 29081-29082 -RRB- denotes ]
T7532 29082-29083 . denotes .
T7533 29083-29250 sentence denotes It is conceivable that loss of pendrin, which may secrete HCO3 - into endolymph, results in an accumulation of HCO3 - and an alkalinization of the intrastrial spaces.
T7534 29084-29086 PRP denotes It
T7535 29087-29089 VBZ denotes is
T7536 29090-29101 JJ denotes conceivable
T7537 29102-29106 IN denotes that
T7539 29107-29111 NN denotes loss
T7540 29112-29114 IN denotes of
T7541 29115-29122 NN denotes pendrin
T7542 29122-29124 , denotes ,
T7543 29124-29129 WDT denotes which
T7545 29130-29133 MD denotes may
T7544 29134-29141 VB denotes secrete
T7546 29142-29146 NN denotes HCO3
T7547 29147-29148 SYM denotes -
T7548 29149-29153 IN denotes into
T7549 29154-29163 NN denotes endolymph
T7550 29163-29165 , denotes ,
T7538 29165-29172 VBZ denotes results
T7551 29173-29175 IN denotes in
T7552 29176-29178 DT denotes an
T7553 29179-29191 NN denotes accumulation
T7554 29192-29194 IN denotes of
T7555 29195-29199 NN denotes HCO3
T7556 29200-29201 SYM denotes -
T7557 29202-29205 CC denotes and
T7558 29206-29208 DT denotes an
T7559 29209-29223 NN denotes alkalinization
T7560 29224-29226 IN denotes of
T7561 29227-29230 DT denotes the
T7563 29231-29242 JJ denotes intrastrial
T7562 29243-29249 NNS denotes spaces
T7564 29249-29250 . denotes .
T7565 29250-29426 sentence denotes This extracellular alkalinization may enhance free radical stress, since it may inhibit the uptake of cysteine and thereby limit production of the protective glutathione [38].
T7566 29251-29255 DT denotes This
T7568 29256-29269 JJ denotes extracellular
T7567 29270-29284 NN denotes alkalinization
T7570 29285-29288 MD denotes may
T7569 29289-29296 VB denotes enhance
T7571 29297-29301 JJ denotes free
T7572 29302-29309 NN denotes radical
T7573 29310-29316 NN denotes stress
T7574 29316-29318 , denotes ,
T7575 29318-29323 IN denotes since
T7577 29324-29326 PRP denotes it
T7578 29327-29330 MD denotes may
T7576 29331-29338 VB denotes inhibit
T7579 29339-29342 DT denotes the
T7580 29343-29349 NN denotes uptake
T7581 29350-29352 IN denotes of
T7582 29353-29361 NN denotes cysteine
T7583 29362-29365 CC denotes and
T7584 29366-29373 RB denotes thereby
T7585 29374-29379 VB denotes limit
T7586 29380-29390 NN denotes production
T7587 29391-29393 IN denotes of
T7588 29394-29397 DT denotes the
T7590 29398-29408 JJ denotes protective
T7589 29409-29420 NN denotes glutathione
T7591 29421-29422 -LRB- denotes [
T7592 29422-29424 CD denotes 38
T7593 29424-29425 -RRB- denotes ]
T7594 29425-29426 . denotes .
T7595 29426-29552 sentence denotes Support for the hypothesis of enhanced free radical stress comes from the observed hyperpigmentation in mice lacking pendrin.
T7596 29427-29434 NN denotes Support
T7598 29435-29438 IN denotes for
T7599 29439-29442 DT denotes the
T7600 29443-29453 NN denotes hypothesis
T7601 29454-29456 IN denotes of
T7602 29457-29465 VBN denotes enhanced
T7604 29466-29470 JJ denotes free
T7605 29471-29478 NN denotes radical
T7603 29479-29485 NN denotes stress
T7597 29486-29491 VBZ denotes comes
T7606 29492-29496 IN denotes from
T7607 29497-29500 DT denotes the
T7609 29501-29509 VBN denotes observed
T7608 29510-29527 NN denotes hyperpigmentation
T7610 29528-29530 IN denotes in
T7611 29531-29535 NNS denotes mice
T7612 29536-29543 VBG denotes lacking
T7613 29544-29551 NN denotes pendrin
T7614 29551-29552 . denotes .
T7615 29552-29696 sentence denotes Strial hyperpigmentation has also been observed in other conditions that are associated with free radical stress, such as acoustic trauma [37].
T7616 29553-29559 JJ denotes Strial
T7617 29560-29577 NN denotes hyperpigmentation
T7619 29578-29581 VBZ denotes has
T7620 29582-29586 RB denotes also
T7621 29587-29591 VBN denotes been
T7618 29592-29600 VBN denotes observed
T7622 29601-29603 IN denotes in
T7623 29604-29609 JJ denotes other
T7624 29610-29620 NNS denotes conditions
T7625 29621-29625 WDT denotes that
T7627 29626-29629 VBP denotes are
T7626 29630-29640 VBN denotes associated
T7628 29641-29645 IN denotes with
T7629 29646-29650 JJ denotes free
T7630 29651-29658 NN denotes radical
T7631 29659-29665 NN denotes stress
T7632 29665-29667 , denotes ,
T7633 29667-29671 JJ denotes such
T7634 29672-29674 IN denotes as
T7635 29675-29683 JJ denotes acoustic
T7636 29684-29690 NN denotes trauma
T7637 29691-29692 -LRB- denotes [
T7638 29692-29694 CD denotes 37
T7639 29694-29695 -RRB- denotes ]
T7640 29695-29696 . denotes .
T7641 29696-29848 sentence denotes Alterations in the cytosolic pH in conjunction with free radical stress may lead to the loss of KCNJ10 protein expression in strial intermediate cells.
T7642 29697-29708 NNS denotes Alterations
T7644 29709-29711 IN denotes in
T7645 29712-29715 DT denotes the
T7647 29716-29725 JJ denotes cytosolic
T7646 29726-29728 NN denotes pH
T7648 29729-29731 IN denotes in
T7649 29732-29743 NN denotes conjunction
T7650 29744-29748 IN denotes with
T7651 29749-29753 JJ denotes free
T7652 29754-29761 NN denotes radical
T7653 29762-29768 NN denotes stress
T7654 29769-29772 MD denotes may
T7643 29773-29777 VB denotes lead
T7655 29778-29780 IN denotes to
T7656 29781-29784 DT denotes the
T7657 29785-29789 NN denotes loss
T7658 29790-29792 IN denotes of
T7659 29793-29799 NN denotes KCNJ10
T7661 29800-29807 NN denotes protein
T7660 29808-29818 NN denotes expression
T7662 29819-29821 IN denotes in
T7663 29822-29828 JJ denotes strial
T7665 29829-29841 JJ denotes intermediate
T7664 29842-29847 NNS denotes cells
T7666 29847-29848 . denotes .
T7667 29848-30015 sentence denotes Function and expression of other K+ channels has been shown to be controlled by the cytosolic pH and free radicals, which encode the metabolic state of the cell [39].
T7668 29849-29857 NN denotes Function
T7670 29858-29861 CC denotes and
T7671 29862-29872 NN denotes expression
T7672 29873-29875 IN denotes of
T7673 29876-29881 JJ denotes other
T7675 29882-29883 NN denotes K
T7676 29883-29884 SYM denotes +
T7674 29885-29893 NNS denotes channels
T7677 29894-29897 VBZ denotes has
T7678 29898-29902 VBN denotes been
T7669 29903-29908 VBN denotes shown
T7679 29909-29911 TO denotes to
T7681 29912-29914 VB denotes be
T7680 29915-29925 VBN denotes controlled
T7682 29926-29928 IN denotes by
T7683 29929-29932 DT denotes the
T7685 29933-29942 JJ denotes cytosolic
T7684 29943-29945 NN denotes pH
T7686 29946-29949 CC denotes and
T7687 29950-29954 JJ denotes free
T7688 29955-29963 NNS denotes radicals
T7689 29963-29965 , denotes ,
T7690 29965-29970 WDT denotes which
T7691 29971-29977 VBP denotes encode
T7692 29978-29981 DT denotes the
T7694 29982-29991 JJ denotes metabolic
T7693 29992-29997 NN denotes state
T7695 29998-30000 IN denotes of
T7696 30001-30004 DT denotes the
T7697 30005-30009 NN denotes cell
T7698 30010-30011 -LRB- denotes [
T7699 30011-30013 CD denotes 39
T7700 30013-30014 -RRB- denotes ]
T7701 30014-30015 . denotes .
T7702 30015-30257 sentence denotes Suppression of the KCNJ10 K+ channel in strial intermediate cells, which is essential for the generation of the endocochlear potential, is probably the direct cause of deafness in Slc26a4-/- mice and patients suffering from Pendred syndrome.
T7703 30016-30027 NN denotes Suppression
T7705 30028-30030 IN denotes of
T7706 30031-30034 DT denotes the
T7708 30035-30041 NN denotes KCNJ10
T7709 30042-30043 NN denotes K
T7710 30043-30044 SYM denotes +
T7707 30045-30052 NN denotes channel
T7711 30053-30055 IN denotes in
T7712 30056-30062 JJ denotes strial
T7714 30063-30075 JJ denotes intermediate
T7713 30076-30081 NNS denotes cells
T7715 30081-30083 , denotes ,
T7716 30083-30088 WDT denotes which
T7717 30089-30091 VBZ denotes is
T7718 30092-30101 JJ denotes essential
T7719 30102-30105 IN denotes for
T7720 30106-30109 DT denotes the
T7721 30110-30120 NN denotes generation
T7722 30121-30123 IN denotes of
T7723 30124-30127 DT denotes the
T7725 30128-30140 JJ denotes endocochlear
T7724 30141-30150 NN denotes potential
T7726 30150-30152 , denotes ,
T7704 30152-30154 VBZ denotes is
T7727 30155-30163 RB denotes probably
T7728 30164-30167 DT denotes the
T7730 30168-30174 JJ denotes direct
T7729 30175-30180 NN denotes cause
T7731 30181-30183 IN denotes of
T7732 30184-30192 NN denotes deafness
T7733 30193-30195 IN denotes in
T7734 30196-30203 NN denotes Slc26a4
T7736 30203-30204 SYM denotes -
T7737 30204-30205 HYPH denotes /
T7738 30205-30206 SYM denotes -
T7735 30207-30211 NNS denotes mice
T7739 30212-30215 CC denotes and
T7740 30216-30224 NNS denotes patients
T7741 30225-30234 VBG denotes suffering
T7742 30235-30239 IN denotes from
T7743 30240-30247 NNP denotes Pendred
T7744 30248-30256 NN denotes syndrome
T7745 30256-30257 . denotes .
T9958 30268-30273 NN denotes Model
T9959 30274-30277 IN denotes for
T9960 30278-30281 DT denotes the
T9961 30282-30286 NN denotes loss
T9962 30287-30289 IN denotes of
T9963 30290-30296 NN denotes KCNJ10
T9964 30297-30299 IN denotes in
T9965 30300-30303 DT denotes the
T9966 30304-30311 NN denotes absence
T9967 30312-30314 IN denotes of
T9968 30315-30322 NN denotes pendrin
T9969 30323-30333 NN denotes expression
T9970 30334-30336 IN denotes in
T9971 30337-30342 NN denotes stria
T9972 30343-30353 NN denotes vascularis
T9973 30353-30354 . denotes .
T9974 30354-30473 sentence denotes Cys, cysteine, Glu, glutamate, Gly, glycine, CA, carbonic anhydrase, GST, glutathione-S-transferase, GSH, glutathione.
T9975 30355-30358 NN denotes Cys
T9976 30358-30360 , denotes ,
T9977 30360-30368 NN denotes cysteine
T9978 30368-30370 , denotes ,
T9979 30370-30373 NN denotes Glu
T9980 30373-30375 , denotes ,
T9981 30375-30384 NN denotes glutamate
T9982 30384-30386 , denotes ,
T9983 30386-30389 NN denotes Gly
T9984 30389-30391 , denotes ,
T9985 30391-30398 NN denotes glycine
T9986 30398-30400 , denotes ,
T9987 30400-30402 NN denotes CA
T9988 30402-30404 , denotes ,
T9989 30404-30412 JJ denotes carbonic
T9990 30413-30422 NN denotes anhydrase
T9991 30422-30424 , denotes ,
T9992 30424-30427 NN denotes GST
T9993 30427-30429 , denotes ,
T9994 30429-30440 NN denotes glutathione
T9996 30440-30441 HYPH denotes -
T9997 30441-30442 NN denotes S
T9998 30442-30443 HYPH denotes -
T9995 30443-30454 NN denotes transferase
T9999 30454-30456 , denotes ,
T10000 30456-30459 NN denotes GSH
T10001 30459-30461 , denotes ,
T10002 30461-30472 NN denotes glutathione
T10003 30472-30473 . denotes .
T7746 31266-31269 DT denotes The
T7748 31270-31285 JJ denotes pre-publication
T7747 31286-31293 NN denotes history
T7750 31294-31297 IN denotes for
T7751 31298-31302 DT denotes this
T7752 31303-31308 NN denotes paper
T7753 31309-31312 MD denotes can
T7754 31313-31315 VB denotes be
T7749 31316-31324 VBN denotes accessed
T7755 31325-31329 RB denotes here
T7756 31329-31330 : denotes :
R1000 T1739 T1738 prep with,infiltrated
R1001 T1740 T1741 compound polyethylene,glycol
R1002 T1741 T1739 pobj glycol,with
R1003 T1742 T1723 punct .,fixed
R1004 T1744 T1745 amod Mid-modiolar,cryosections
R1005 T1745 T1746 nsubjpass cryosections,blocked
R1006 T1747 T1748 punct (,CM3050S
R1007 T1748 T1745 parataxis CM3050S,cryosections
R1008 T1749 T1750 nummod 12,μm
R1009 T1750 T1748 nmod μm,CM3050S
R1010 T1751 T1748 punct ", ",CM3050S
R1011 T1752 T1748 punct ", ",CM3050S
R1012 T1753 T1748 npadvmod Leica,CM3050S
R1013 T1754 T1748 punct ", ",CM3050S
R1014 T1755 T1748 npadvmod Nussloch,CM3050S
R1015 T1756 T1748 punct ", ",CM3050S
R1016 T1757 T1748 npadvmod Germany,CM3050S
R1017 T1758 T1748 punct ),CM3050S
R1018 T1759 T1746 auxpass were,blocked
R1019 T1760 T1746 prep in,blocked
R1020 T1761 T1760 pobj PBS,in
R1021 T1762 T1746 prep with,blocked
R1022 T1763 T1764 nummod 0.2,%
R1023 T1764 T1765 compound %,X
R1024 T1765 T1762 pobj X,with
R1025 T1766 T1765 compound Triton,X
R1026 T1767 T1765 punct -,X
R1027 T1768 T1769 punct (,TX
R1028 T1769 T1765 parataxis TX,X
R1029 T1770 T1769 compound PBS,TX
R1030 T1771 T1769 punct -,TX
R1031 T1772 T1769 punct ),TX
R1032 T1773 T1765 cc and,X
R1033 T1774 T1775 nummod 10,%
R1034 T1775 T1776 nmod %,albumin
R1035 T1776 T1765 conj albumin,X
R1036 T1777 T1776 amod bovine,albumin
R1037 T1778 T1776 compound serum,albumin
R1038 T1779 T1746 punct .,blocked
R1039 T1781 T1782 nsubjpass Slides,incubated
R1040 T1783 T1782 auxpass were,incubated
R1041 T1784 T1782 advmod overnight,incubated
R1042 T1785 T1782 prep at,incubated
R1043 T1786 T1787 nummod 4,°C
R1044 T1787 T1785 pobj °C,at
R1045 T1788 T1782 prep with,incubated
R1046 T1789 T1790 amod primary,antibodies
R1047 T1790 T1788 pobj antibodies,with
R1048 T1791 T1782 prep in,incubated
R1049 T1792 T1793 compound PBS,TX
R1050 T1793 T1791 pobj TX,in
R1051 T1794 T1793 punct -,TX
R1052 T1795 T1782 prep with,incubated
R1053 T1796 T1797 quantmod 1,3
R1054 T1797 T1799 nummod 3,%
R1055 T1798 T1797 punct –,3
R1056 T1799 T1800 compound %,BSA
R1057 T1800 T1795 pobj BSA,with
R1058 T1801 T1802 punct [,h766
R1059 T1802 T1782 parataxis h766,incubated
R1060 T1803 T1804 compound rabbit,anti-pendrin
R1061 T1804 T1802 dep anti-pendrin,h766
R1062 T1805 T1802 punct ", ",h766
R1063 T1806 T1802 dep 1,h766
R1064 T1807 T1808 punct :,500
R1065 T1808 T1806 prep 500,1
R1066 T1809 T1802 punct (,h766
R1067 T1810 T1811 punct –,780
R1068 T1811 T1802 prep 780,h766
R1069 T1812 T1802 punct ),h766
R1070 T1813 T1802 punct ;,h766
R1071 T1814 T1815 compound rat,anti-ZO
R1072 T1815 T1816 dep anti-ZO,Chemicon
R1073 T1816 T1802 conj Chemicon,h766
R1074 T1817 T1815 punct -,anti-ZO
R1075 T1818 T1815 nummod 1,anti-ZO
R1076 T1819 T1816 punct ", ",Chemicon
R1077 T1820 T1816 dep 1,Chemicon
R1078 T1821 T1822 punct :,100
R1079 T1822 T1820 prep 100,1
R1080 T1823 T1816 punct (,Chemicon
R1081 T1824 T1816 punct ", ",Chemicon
R1082 T1825 T1816 npadvmod Temecula,Chemicon
R1083 T1826 T1816 punct ", ",Chemicon
R1084 T1827 T1816 npadvmod CA,Chemicon
R1085 T1828 T1816 punct ),Chemicon
R1086 T1829 T1816 punct ;,Chemicon
R1087 T1830 T1831 compound goat,anti-KCNQ1
R1088 T1831 T1832 dep anti-KCNQ1,C20
R1089 T1832 T1816 conj C20,Chemicon
R1090 T1833 T1832 punct ", ",C20
R1091 T1834 T1832 dep 1,C20
R1092 T1835 T1836 punct :,200
R1093 T1836 T1834 prep 200,1
R1094 T1837 T1832 punct (,C20
R1095 T1838 T1832 punct ", ",C20
R1096 T1839 T1840 compound Santa,Cruz
R1097 T1840 T1841 compound Cruz,Biotechnology
R1098 T1841 T1832 npadvmod Biotechnology,C20
R1099 T1842 T1832 punct ", ",C20
R1100 T1843 T1844 compound Santa,Cruz
R1101 T1844 T1832 npadvmod Cruz,C20
R1102 T1845 T1832 punct ", ",C20
R1103 T1846 T1832 npadvmod CA,C20
R1104 T1847 T1832 punct ),C20
R1105 T1848 T1832 punct ", ",C20
R1106 T1849 T1850 compound rabbit,anti-KCNE1
R1107 T1850 T1851 dep anti-KCNE1,Alomone
R1108 T1851 T1832 conj Alomone,C20
R1109 T1852 T1851 punct ", ",Alomone
R1110 T1853 T1851 dep 1,Alomone
R1111 T1854 T1855 punct :,200
R1112 T1855 T1853 prep 200,1
R1113 T1856 T1851 punct (,Alomone
R1114 T1857 T1851 punct ", ",Alomone
R1115 T1858 T1851 npadvmod Jerusalem,Alomone
R1116 T1859 T1851 punct ", ",Alomone
R1117 T1860 T1851 npadvmod Israel,Alomone
R1118 T1861 T1851 punct ),Alomone
R1119 T1862 T1851 punct ;,Alomone
R1120 T1863 T1864 compound rabbit,anti-KCNJ10
R1121 T1864 T1865 dep anti-KCNJ10,Alomone
R1122 T1865 T1851 conj Alomone,Alomone
R1123 T1866 T1865 punct ", ",Alomone
R1124 T1867 T1865 dep 1,Alomone
R1125 T1868 T1869 punct :,300
R1126 T1869 T1867 prep 300,1
R1127 T1870 T1865 punct (,Alomone
R1128 T1871 T1865 punct ),Alomone
R1129 T1872 T1865 punct ;,Alomone
R1130 T1873 T1874 compound rabbit,anti-SLC12A2
R1131 T1874 T1875 dep anti-SLC12A2,Chemicon
R1132 T1875 T1865 conj Chemicon,Alomone
R1133 T1876 T1875 punct ", ",Chemicon
R1134 T1877 T1875 dep 1,Chemicon
R1135 T1878 T1879 punct :,100
R1136 T1879 T1877 prep 100,1
R1137 T1880 T1875 punct (,Chemicon
R1138 T1881 T1875 punct ),Chemicon
R1139 T1882 T1875 punct ;,Chemicon
R1140 T1883 T1875 cc and,Chemicon
R1141 T1884 T1885 compound rabbit,anti-connexin
R1142 T1885 T1886 dep anti-connexin,Zymed
R1143 T1886 T1875 conj Zymed,Chemicon
R1144 T1887 T1885 nummod 26,anti-connexin
R1145 T1888 T1886 punct ", ",Zymed
R1146 T1889 T1886 dep 1,Zymed
R1147 T1890 T1891 punct :,100
R1148 T1891 T1889 prep 100,1
R1149 T1892 T1886 punct (,Zymed
R1150 T1893 T1886 punct ", ",Zymed
R1151 T1894 T1895 compound San,Francisco
R1152 T1895 T1886 npadvmod Francisco,Zymed
R1153 T1896 T1886 punct ", ",Zymed
R1154 T1897 T1886 npadvmod CA,Zymed
R1155 T1898 T1886 punct ),Zymed
R1156 T1899 T1886 punct ],Zymed
R1157 T1900 T1782 punct .,incubated
R1158 T1902 T1903 nsubjpass Slides,washed
R1159 T1904 T1903 auxpass were,washed
R1160 T1905 T1903 prep in,washed
R1161 T1906 T1907 compound PBS,TX
R1162 T1907 T1905 pobj TX,in
R1163 T1908 T1907 punct -,TX
R1164 T1909 T1903 cc and,washed
R1165 T1910 T1903 conj incubated,washed
R1166 T1911 T1910 prep for,incubated
R1167 T1912 T1913 nummod 1,h
R1168 T1913 T1911 pobj h,for
R1169 T1914 T1910 prep at,incubated
R1170 T1915 T1916 nummod 25,°C
R1171 T1916 T1914 pobj °C,at
R1172 T1917 T1910 prep with,incubated
R1173 T1918 T1919 amod appropriate,antibodies
R1174 T1919 T1917 pobj antibodies,with
R1175 T1920 T1919 amod secondary,antibodies
R1176 T1921 T1919 prep at,antibodies
R1177 T1922 T1923 det a,dilution
R1178 T1923 T1921 pobj dilution,at
R1179 T1924 T1923 nummod 1,dilution
R1180 T1925 T1926 punct :,1000
R1181 T1926 T1924 prep 1000,1
R1182 T1927 T1923 prep in,dilution
R1183 T1928 T1929 compound PBS,TX
R1184 T1929 T1927 pobj TX,in
R1185 T1930 T1929 punct -,TX
R1186 T1931 T1923 prep with,dilution
R1187 T1932 T1933 quantmod 1,3
R1188 T1933 T1935 nummod 3,%
R1189 T1934 T1933 punct –,3
R1190 T1935 T1936 compound %,BSA
R1191 T1936 T1931 pobj BSA,with
R1192 T1937 T1938 punct [,Probes
R1193 T1938 T1910 parataxis Probes,incubated
R1194 T1939 T1940 nmod donkey,Alexa
R1195 T1940 T1938 dep Alexa,Probes
R1196 T1941 T1939 amod anti-rabbit,donkey
R1197 T1942 T1940 nummod 488,Alexa
R1198 T1943 T1940 punct ", ",Alexa
R1199 T1944 T1945 nmod chicken,Alexa
R1200 T1945 T1940 conj Alexa,Alexa
R1201 T1946 T1944 amod anti-rat,chicken
R1202 T1947 T1945 nummod 594,Alexa
R1203 T1948 T1945 punct ", ",Alexa
R1204 T1949 T1945 cc and,Alexa
R1205 T1950 T1951 nmod chicken,Alexa
R1206 T1951 T1945 conj Alexa,Alexa
R1207 T1952 T1950 amod anti-goat,chicken
R1208 T1953 T1951 nummod 594,Alexa
R1209 T1954 T1938 punct (,Probes
R1210 T1955 T1938 compound Molecular,Probes
R1211 T1956 T1938 punct ", ",Probes
R1212 T1957 T1938 npadvmod Eugene,Probes
R1213 T1958 T1938 punct ", ",Probes
R1214 T1959 T1938 npadvmod OR,Probes
R1215 T1960 T1938 punct ),Probes
R1216 T1961 T1938 punct ],Probes
R1217 T1962 T1903 punct .,washed
R1218 T1964 T1965 compound Actin,filaments
R1219 T1965 T1966 nsubjpass filaments,visualized
R1220 T1967 T1966 auxpass were,visualized
R1221 T1968 T1966 prep by,visualized
R1222 T1969 T1968 pcomp staining,by
R1223 T1970 T1969 prep with,staining
R1224 T1971 T1972 npadvmod Alexa,conjugated
R1225 T1972 T1974 amod conjugated,phalloidin
R1226 T1973 T1971 nummod 488,Alexa
R1227 T1974 T1970 pobj phalloidin,with
R1228 T1975 T1976 punct (,Probes
R1229 T1976 T1974 parataxis Probes,phalloidin
R1230 T1977 T1976 compound Molecular,Probes
R1231 T1978 T1976 punct ),Probes
R1232 T1979 T1966 punct .,visualized
R1233 T1981 T1982 prep After,washed
R1234 T1983 T1981 pobj incubation,After
R1235 T1984 T1982 punct ", ",washed
R1236 T1985 T1982 nsubjpass slides,washed
R1237 T1986 T1982 auxpass were,washed
R1238 T1987 T1982 prep with,washed
R1239 T1988 T1989 compound PBS,TX
R1240 T1989 T1987 pobj TX,with
R1241 T1990 T1989 punct -,TX
R1242 T1991 T1982 punct ", ",washed
R1243 T1992 T1982 conj mounted,washed
R1244 T1993 T1992 prep with,mounted
R1245 T1994 T1993 pobj FluorSave,with
R1246 T1995 T1996 punct (,Calbiochem
R1247 T1996 T1994 parataxis Calbiochem,FluorSave
R1248 T1997 T1996 punct ", ",Calbiochem
R1249 T1998 T1999 compound La,Jolla
R1250 T1999 T1996 npadvmod Jolla,Calbiochem
R1251 T2000 T1996 punct ", ",Calbiochem
R1252 T2001 T1996 npadvmod CA,Calbiochem
R1253 T2002 T1996 punct ),Calbiochem
R1254 T2003 T1992 punct ", ",mounted
R1255 T2004 T1992 cc and,mounted
R1256 T2005 T1992 conj viewed,mounted
R1257 T2006 T2005 prep by,viewed
R1258 T2007 T2008 amod confocal,microscopy
R1259 T2008 T2006 pobj microscopy,by
R1260 T2009 T2010 punct (,Pascal
R1261 T2010 T2008 parataxis Pascal,microscopy
R1262 T2011 T2010 nmod LSM,Pascal
R1263 T2012 T2010 nummod 5,Pascal
R1264 T2013 T2010 cc or,Pascal
R1265 T2014 T2015 nmod LSM,Meta
R1266 T2015 T2010 conj Meta,Pascal
R1267 T2016 T2015 nummod 510,Meta
R1268 T2017 T2010 punct ", ",Pascal
R1269 T2018 T2019 compound Carl,Zeiss
R1270 T2019 T2010 npadvmod Zeiss,Pascal
R1271 T2020 T2010 punct ", ",Pascal
R1272 T2021 T2010 npadvmod Jena,Pascal
R1273 T2022 T2021 cc or,Jena
R1274 T2023 T2021 conj Göttingen,Jena
R1275 T2024 T2010 punct ", ",Pascal
R1276 T2025 T2010 npadvmod Germany,Pascal
R1277 T2026 T2010 punct ),Pascal
R1278 T2027 T1982 punct .,washed
R1279 T2029 T2030 compound Laser,scanning
R1280 T2030 T2031 compound scanning,images
R1281 T2031 T2033 nsubjpass images,collected
R1282 T2032 T2031 compound brightfield,images
R1283 T2034 T2033 auxpass were,collected
R1284 T2035 T2036 aux to,document
R1285 T2036 T2033 advcl document,collected
R1286 T2037 T2038 amod structural,preservation
R1287 T2038 T2036 dobj preservation,document
R1288 T2039 T2033 punct ", ",collected
R1289 T2040 T2033 prep for,collected
R1290 T2041 T2042 amod morphometric,analysis
R1291 T2042 T2040 pobj analysis,for
R1292 T2043 T2040 cc and,for
R1293 T2044 T2040 conj for,for
R1294 T2045 T2044 pobj analysis,for
R1295 T2046 T2045 prep of,analysis
R1296 T2047 T2046 pobj pigmentation,of
R1297 T2048 T2033 punct .,collected
R1298 T2210 T2211 amod Quantitative,PCR
R1299 T2212 T2211 compound RT,PCR
R1300 T2213 T2211 punct -,PCR
R1301 T2215 T2216 nsubjpass Animals,anesthetized
R1302 T2217 T2216 auxpass were,anesthetized
R1303 T2218 T2216 advmod deeply,anesthetized
R1304 T2219 T2216 prep with,anesthetized
R1305 T2220 T2221 compound sodium,pentobarbital
R1306 T2221 T2219 pobj pentobarbital,with
R1307 T2222 T2223 punct (,mg
R1308 T2223 T2221 parataxis mg,pentobarbital
R1309 T2224 T2223 nummod 100,mg
R1310 T2225 T2226 punct /,kg
R1311 T2226 T2223 prep kg,mg
R1312 T2227 T2223 advmod i.p.,mg
R1313 T2228 T2223 punct ),mg
R1314 T2229 T2216 punct .,anesthetized
R1315 T2231 T2232 nsubjpass Kidneys,removed
R1316 T2233 T2231 cc and,Kidneys
R1317 T2234 T2231 conj brain,Kidneys
R1318 T2235 T2232 auxpass were,removed
R1319 T2236 T2232 cc and,removed
R1320 T2237 T2232 conj pulverized,removed
R1321 T2238 T2237 prep in,pulverized
R1322 T2239 T2240 compound liquid,N2
R1323 T2240 T2238 pobj N2,in
R1324 T2241 T2232 punct .,removed
R1325 T2243 T2244 amod Temporal,bones
R1326 T2244 T2245 nsubjpass bones,removed
R1327 T2246 T2245 auxpass were,removed
R1328 T2247 T2245 cc and,removed
R1329 T2248 T2249 nummod two,preparations
R1330 T2249 T2250 nsubjpass preparations,obtained
R1331 T2250 T2245 conj obtained,removed
R1332 T2251 T2249 prep of,preparations
R1333 T2252 T2253 amod inner,tissues
R1334 T2253 T2251 pobj tissues,of
R1335 T2254 T2253 compound ear,tissues
R1336 T2255 T2250 auxpass were,obtained
R1337 T2256 T2250 prep by,obtained
R1338 T2257 T2256 pobj microdissection,by
R1339 T2258 T2250 punct : ,obtained
R1340 T2259 T2260 punct (,1
R1341 T2260 T2261 meta 1,vascularis
R1342 T2261 T2250 dobj vascularis,obtained
R1343 T2262 T2260 punct ),1
R1344 T2263 T2261 compound stria,vascularis
R1345 T2264 T2261 prep without,vascularis
R1346 T2265 T2266 amod spiral,ligament
R1347 T2266 T2264 pobj ligament,without
R1348 T2267 T2261 punct ", ",vascularis
R1349 T2268 T2261 cc and,vascularis
R1350 T2269 T2270 punct (,2
R1351 T2270 T2271 meta 2,ganglia
R1352 T2271 T2261 conj ganglia,vascularis
R1353 T2272 T2270 punct ),2
R1354 T2273 T2271 amod spiral,ganglia
R1355 T2274 T2271 prep including,ganglia
R1356 T2275 T2276 det the,bone
R1357 T2276 T2274 pobj bone,including
R1358 T2277 T2276 amod surrounding,bone
R1359 T2278 T2276 cc and,bone
R1360 T2279 T2280 det the,organ
R1361 T2280 T2276 conj organ,bone
R1362 T2281 T2280 prep of,organ
R1363 T2282 T2281 pobj Corti,of
R1364 T2283 T2250 punct .,obtained
R1365 T2285 T2286 det The,medium
R1366 T2286 T2288 nsubjpass medium,changed
R1367 T2287 T2286 compound dissection,medium
R1368 T2289 T2288 auxpass was,changed
R1369 T2290 T2288 advmod twice,changed
R1370 T2291 T2288 prep during,changed
R1371 T2292 T2293 det the,microdissection
R1372 T2293 T2291 pobj microdissection,during
R1373 T2294 T2288 cc and,changed
R1374 T2295 T2296 amod isolated,tissues
R1375 T2296 T2297 nsubjpass tissues,washed
R1376 T2297 T2288 conj washed,changed
R1377 T2298 T2297 auxpass were,washed
R1378 T2299 T2300 nummod three,times
R1379 T2300 T2297 npadvmod times,washed
R1380 T2301 T2302 aux to,minimize
R1381 T2302 T2297 advcl minimize,washed
R1382 T2303 T2302 dobj cross-contamination,minimize
R1383 T2304 T2297 punct .,washed
R1384 T2306 T2307 det The,genotype
R1385 T2307 T2312 nsubjpass genotype,verified
R1386 T2308 T2307 nmod Slc26a4,genotype
R1387 T2309 T2308 punct -,Slc26a4
R1388 T2310 T2308 punct /,Slc26a4
R1389 T2311 T2308 punct -,Slc26a4
R1390 T2313 T2312 auxpass was,verified
R1391 T2314 T2312 prep by,verified
R1392 T2315 T2316 det the,observation
R1393 T2316 T2314 pobj observation,by
R1394 T2317 T2316 prep of,observation
R1395 T2318 T2319 amod large,otoconia
R1396 T2319 T2317 pobj otoconia,of
R1397 T2320 T2316 prep in,observation
R1398 T2321 T2322 det the,macula
R1399 T2322 T2320 pobj macula,in
R1400 T2323 T2322 amod utricular,macula
R1401 T2324 T2325 punct [,14
R1402 T2325 T2312 parataxis 14,verified
R1403 T2326 T2325 punct ],14
R1404 T2327 T2312 punct .,verified
R1405 T2329 T2330 amod Total,RNA
R1406 T2330 T2331 nsubjpass RNA,isolated
R1407 T2332 T2331 auxpass was,isolated
R1408 T2333 T2331 cc and,isolated
R1409 T2334 T2335 amod residual,contamination
R1410 T2335 T2337 nsubjpass contamination,removed
R1411 T2336 T2335 compound DNA,contamination
R1412 T2337 T2331 conj removed,isolated
R1413 T2338 T2337 auxpass was,removed
R1414 T2339 T2337 prep by,removed
R1415 T2340 T2341 amod enzymatic,digestion
R1416 T2341 T2339 pobj digestion,by
R1417 T2342 T2343 punct (,micro
R1418 T2343 T2337 parataxis micro,removed
R1419 T2344 T2343 compound RNeasy,micro
R1420 T2345 T2343 punct ", ",micro
R1421 T2346 T2343 npadvmod Qiagen,micro
R1422 T2347 T2343 punct ", ",micro
R1423 T2348 T2343 npadvmod Valencia,micro
R1424 T2349 T2343 punct ", ",micro
R1425 T2350 T2343 npadvmod CA,micro
R1426 T2351 T2343 punct ),micro
R1427 T2352 T2337 punct .,removed
R1428 T2354 T2355 nsubjpass Quality,determined
R1429 T2356 T2354 cc and,Quality
R1430 T2357 T2354 conj quantity,Quality
R1431 T2358 T2354 prep of,Quality
R1432 T2359 T2360 compound 18S,rRNA
R1433 T2360 T2358 pobj rRNA,of
R1434 T2361 T2360 acl obtained,rRNA
R1435 T2362 T2361 prep from,obtained
R1436 T2363 T2362 pobj kidneys,from
R1437 T2364 T2363 cc and,kidneys
R1438 T2365 T2363 conj brain,kidneys
R1439 T2366 T2355 auxpass were,determined
R1440 T2367 T2368 punct (,Assay
R1441 T2368 T2355 parataxis Assay,determined
R1442 T2369 T2368 compound Nano,Assay
R1443 T2370 T2368 punct ", ",Assay
R1444 T2371 T2372 nummod 2100,Bioanalyzer
R1445 T2372 T2368 appos Bioanalyzer,Assay
R1446 T2373 T2368 punct ", ",Assay
R1447 T2374 T2368 npadvmod Agilent,Assay
R1448 T2375 T2368 punct ", ",Assay
R1449 T2376 T2377 compound Palo,Alto
R1450 T2377 T2368 npadvmod Alto,Assay
R1451 T2378 T2368 punct ", ",Assay
R1452 T2379 T2368 npadvmod CA,Assay
R1453 T2380 T2368 punct ),Assay
R1454 T2381 T2355 punct .,determined
R1455 T2383 T2384 advmod Further,assessed
R1456 T2385 T2384 punct ", ",assessed
R1457 T2386 T2387 det the,quality
R1458 T2387 T2384 nsubjpass quality,assessed
R1459 T2388 T2387 prep of,quality
R1460 T2389 T2390 compound RNA,preparations
R1461 T2390 T2388 pobj preparations,of
R1462 T2391 T2390 acl obtained,preparations
R1463 T2392 T2391 prep from,obtained
R1464 T2393 T2394 compound stria,vascularis
R1465 T2394 T2392 pobj vascularis,from
R1466 T2395 T2394 cc and,vascularis
R1467 T2396 T2397 amod spiral,ganglia
R1468 T2397 T2394 conj ganglia,vascularis
R1469 T2398 T2384 auxpass was,assessed
R1470 T2399 T2400 punct (,Assay
R1471 T2400 T2384 parataxis Assay,assessed
R1472 T2401 T2400 compound Pico,Assay
R1473 T2402 T2400 punct ", ",Assay
R1474 T2403 T2404 nummod 2100,Bioanalyzer
R1475 T2404 T2400 appos Bioanalyzer,Assay
R1476 T2405 T2400 punct ),Assay
R1477 T2406 T2384 punct .,assessed
R1478 T2408 T2409 amod Real,time
R1479 T2409 T2410 compound time,PCR
R1480 T2410 T2413 nsubjpass PCR,performed
R1481 T2411 T2410 compound RT,PCR
R1482 T2412 T2410 punct -,PCR
R1483 T2414 T2413 auxpass was,performed
R1484 T2415 T2413 prep on,performed
R1485 T2416 T2415 pobj RNA,on
R1486 T2417 T2416 acl obtained,RNA
R1487 T2418 T2417 prep from,obtained
R1488 T2419 T2420 amod individual,animals
R1489 T2420 T2418 pobj animals,from
R1490 T2421 T2422 punct (,Cycler
R1491 T2422 T2417 parataxis Cycler,obtained
R1492 T2423 T2424 compound OneStep,PCR
R1493 T2424 T2422 dep PCR,Cycler
R1494 T2425 T2424 compound RT,PCR
R1495 T2426 T2424 punct -,PCR
R1496 T2427 T2424 punct ", ",PCR
R1497 T2428 T2424 npadvmod Qiagen,PCR
R1498 T2429 T2422 punct ;,Cycler
R1499 T2430 T2422 compound Smart,Cycler
R1500 T2431 T2422 punct ", ",Cycler
R1501 T2432 T2422 npadvmod Cepheid,Cycler
R1502 T2433 T2422 punct ", ",Cycler
R1503 T2434 T2422 npadvmod Sunnyvale,Cycler
R1504 T2435 T2422 punct ", ",Cycler
R1505 T2436 T2422 npadvmod CA,Cycler
R1506 T2437 T2422 punct ),Cycler
R1507 T2438 T2413 prep in,performed
R1508 T2439 T2440 det the,presence
R1509 T2440 T2438 pobj presence,in
R1510 T2441 T2440 prep of,presence
R1511 T2442 T2443 nummod 0.2,SYBR
R1512 T2443 T2445 nmod SYBR,I
R1513 T2444 T2443 punct ×,SYBR
R1514 T2445 T2441 pobj I,of
R1515 T2446 T2445 amod green,I
R1516 T2447 T2448 punct (,Probes
R1517 T2448 T2445 parataxis Probes,I
R1518 T2449 T2448 compound Molecular,Probes
R1519 T2450 T2448 punct ),Probes
R1520 T2451 T2413 punct .,performed
R1521 T2453 T2454 nsubjpass Transcripts,amplified
R1522 T2455 T2453 prep of,Transcripts
R1523 T2456 T2457 compound 18S,rRNA
R1524 T2457 T2455 pobj rRNA,of
R1525 T2458 T2457 cc and,rRNA
R1526 T2459 T2457 conj mRNA,rRNA
R1527 T2460 T2453 prep for,Transcripts
R1528 T2461 T2462 det the,channels
R1529 T2462 T2460 pobj channels,for
R1530 T2463 T2462 nmod K,channels
R1531 T2464 T2463 punct +,K
R1532 T2465 T2462 appos KCNJ10,channels
R1533 T2466 T2465 punct ", ",KCNJ10
R1534 T2467 T2465 conj KCNQ1,KCNJ10
R1535 T2468 T2467 cc and,KCNQ1
R1536 T2469 T2467 conj KCNQ4,KCNQ1
R1537 T2470 T2454 auxpass were,amplified
R1538 T2471 T2454 advcl using,amplified
R1539 T2472 T2473 npadvmod gene,specific
R1540 T2473 T2475 amod specific,primers
R1541 T2474 T2473 punct -,specific
R1542 T2475 T2471 dobj primers,using
R1543 T2476 T2477 punct (,Table
R1544 T2477 T2454 parataxis Table,amplified
R1545 T2478 T2477 nummod 1,Table
R1546 T2479 T2477 punct ),Table
R1547 T2480 T2454 punct .,amplified
R1548 T2482 T2483 nsubjpass RT,performed
R1549 T2484 T2483 auxpass was,performed
R1550 T2485 T2483 prep for,performed
R1551 T2486 T2487 nummod 30,min
R1552 T2487 T2485 pobj min,for
R1553 T2488 T2483 prep at,performed
R1554 T2489 T2490 nummod 50,°C
R1555 T2490 T2488 pobj °C,at
R1556 T2491 T2483 cc and,performed
R1557 T2492 T2493 nummod 15,min
R1558 T2493 T2483 conj min,performed
R1559 T2494 T2493 prep at,min
R1560 T2495 T2496 nummod 95,°C
R1561 T2496 T2494 pobj °C,at
R1562 T2497 T2483 punct .,performed
R1563 T2499 T2500 nsubj PCR,consisted
R1564 T2501 T2500 prep of,consisted
R1565 T2502 T2503 nummod 50,cycles
R1566 T2503 T2501 pobj cycles,of
R1567 T2504 T2503 prep of,cycles
R1568 T2505 T2506 nummod 1,min
R1569 T2506 T2504 pobj min,of
R1570 T2507 T2506 prep at,min
R1571 T2508 T2509 nummod 60,°C
R1572 T2509 T2507 pobj °C,at
R1573 T2510 T2506 punct ", ",min
R1574 T2511 T2512 nummod 1,min
R1575 T2512 T2506 conj min,min
R1576 T2513 T2512 prep at,min
R1577 T2514 T2515 nummod 72,°C
R1578 T2515 T2513 pobj °C,at
R1579 T2516 T2512 punct ", ",min
R1580 T2517 T2512 conj 7s,min
R1581 T2518 T2517 amod heating,7s
R1582 T2519 T2517 prep to,7s
R1583 T2520 T2521 amod hot,measurement
R1584 T2521 T2523 compound measurement,temperature
R1585 T2522 T2521 punct -,measurement
R1586 T2523 T2519 pobj temperature,to
R1587 T2524 T2517 punct ", ",7s
R1588 T2525 T2526 compound 13s,measurement
R1589 T2526 T2517 conj measurement,7s
R1590 T2527 T2526 compound hot,measurement
R1591 T2528 T2526 punct -,measurement
R1592 T2529 T2526 prep at,measurement
R1593 T2530 T2531 quantmod 3,5
R1594 T2531 T2533 nummod 5,°C
R1595 T2532 T2531 punct –,5
R1596 T2533 T2534 npadvmod °C,below
R1597 T2534 T2529 prep below,at
R1598 T2535 T2536 compound product,temperature
R1599 T2536 T2534 pobj temperature,below
R1600 T2537 T2536 compound melting,temperature
R1601 T2538 T2539 punct (,Table
R1602 T2539 T2536 parataxis Table,temperature
R1603 T2540 T2539 nummod 1,Table
R1604 T2541 T2539 punct ),Table
R1605 T2542 T2526 cc and,measurement
R1606 T2543 T2544 nummod 1,min
R1607 T2544 T2526 conj min,measurement
R1608 T2545 T2544 prep at,min
R1609 T2546 T2547 nummod 94,°C
R1610 T2547 T2545 pobj °C,at
R1611 T2548 T2500 punct .,consisted
R1612 T2550 T2551 amod Hot,measurements
R1613 T2551 T2553 nsubjpass measurements,performed
R1614 T2552 T2551 punct -,measurements
R1615 T2554 T2553 auxpass were,performed
R1616 T2555 T2556 aux to,eliminate
R1617 T2556 T2553 advcl eliminate,performed
R1618 T2557 T2558 det the,detection
R1619 T2558 T2556 dobj detection,eliminate
R1620 T2559 T2558 prep of,detection
R1621 T2560 T2561 compound primer,dimers
R1622 T2561 T2559 pobj dimers,of
R1623 T2562 T2561 punct -,dimers
R1624 T2563 T2564 dep that,had
R1625 T2564 T2561 relcl had,dimers
R1626 T2565 T2566 compound melting,temperatures
R1627 T2566 T2564 dobj temperatures,had
R1628 T2567 T2564 prep between,had
R1629 T2568 T2569 nummod 72,°C
R1630 T2569 T2567 pobj °C,between
R1631 T2570 T2568 cc and,72
R1632 T2571 T2568 conj 75,72
R1633 T2572 T2573 punct [,15
R1634 T2573 T2564 parataxis 15,had
R1635 T2574 T2573 punct ],15
R1636 T2575 T2553 punct .,performed
R1637 T2577 T2578 nsubjpass PCR,followed
R1638 T2579 T2578 auxpass was,followed
R1639 T2580 T2578 agent by,followed
R1640 T2581 T2582 det a,melt
R1641 T2582 T2580 pobj melt,by
R1642 T2583 T2584 punct (,°C
R1643 T2584 T2582 parataxis °C,melt
R1644 T2585 T2586 quantmod 60,95
R1645 T2586 T2584 nummod 95,°C
R1646 T2587 T2586 quantmod to,95
R1647 T2588 T2584 punct ),°C
R1648 T2589 T2578 punct .,followed
R1649 T2591 T2592 det The,generation
R1650 T2592 T2593 nsubjpass generation,checked
R1651 T2594 T2592 prep of,generation
R1652 T2595 T2596 det a,product
R1653 T2596 T2594 pobj product,of
R1654 T2597 T2596 amod single,product
R1655 T2598 T2596 prep of,product
R1656 T2599 T2600 amod appropriate,size
R1657 T2600 T2598 pobj size,of
R1658 T2601 T2593 auxpass was,checked
R1659 T2602 T2593 advmod routinely,checked
R1660 T2603 T2593 prep by,checked
R1661 T2604 T2605 det the,presence
R1662 T2605 T2603 pobj presence,by
R1663 T2606 T2605 prep of,presence
R1664 T2607 T2608 det a,peak
R1665 T2608 T2606 pobj peak,of
R1666 T2609 T2608 amod single,peak
R1667 T2610 T2608 compound melt,peak
R1668 T2611 T2603 cc and,by
R1669 T2612 T2603 conj by,by
R1670 T2613 T2614 compound agarose,gel
R1671 T2614 T2615 compound gel,electrophoresis
R1672 T2615 T2612 pobj electrophoresis,by
R1673 T2616 T2615 punct -,electrophoresis
R1674 T2617 T2593 punct .,checked
R1675 T2619 T2620 compound Product,identity
R1676 T2620 T2621 nsubjpass identity,confirmed
R1677 T2622 T2621 auxpass was,confirmed
R1678 T2623 T2621 prep by,confirmed
R1679 T2624 T2623 pobj sequencing,by
R1680 T2625 T2621 punct .,confirmed
R1681 T2627 T2628 compound "Table 1 Primers Template",molecules
R1682 T2628 T2629 nsubjpass molecules,quantified
R1683 T2630 T2628 punct (,molecules
R1684 T2631 T2628 appos T,molecules
R1685 T2632 T2628 punct ),molecules
R1686 T2633 T2629 auxpass were,quantified
R1687 T2634 T2629 prep according,quantified
R1688 T2635 T2634 prep to,according
R1689 T2636 T2637 nsubj T,P
R1690 T2637 T2635 pcomp P,to
R1691 T2638 T2637 punct =,P
R1692 T2639 T2640 punct /,Ct
R1693 T2640 T2637 prep Ct,P
R1694 T2641 T2640 punct (,Ct
R1695 T2642 T2640 nmod F,Ct
R1696 T2643 T2640 punct ^,Ct
R1697 T2644 T2640 punct ),Ct
R1698 T2645 T2646 advmod where,is
R1699 T2646 T2637 advcl is,P
R1700 T2647 T2646 nsubj P,is
R1701 T2648 T2649 det the,number
R1702 T2649 T2646 attr number,is
R1703 T2650 T2649 prep of,number
R1704 T2651 T2652 compound product,molecules
R1705 T2652 T2650 pobj molecules,of
R1706 T2653 T2646 punct ", ",is
R1707 T2654 T2655 nsubj F,is
R1708 T2655 T2646 conj is,is
R1709 T2656 T2657 det the,fidelity
R1710 T2657 T2655 attr fidelity,is
R1711 T2658 T2657 prep of,fidelity
R1712 T2659 T2660 det the,reaction
R1713 T2660 T2658 pobj reaction,of
R1714 T2661 T2655 cc and,is
R1715 T2662 T2663 nsubj Ct,is
R1716 T2663 T2655 conj is,is
R1717 T2664 T2665 det the,cycle
R1718 T2665 T2663 attr cycle,is
R1719 T2666 T2667 prep at,reaches
R1720 T2667 T2665 relcl reaches,cycle
R1721 T2668 T2666 pobj which,at
R1722 T2669 T2670 det the,number
R1723 T2670 T2667 nsubj number,reaches
R1724 T2671 T2670 prep of,number
R1725 T2672 T2673 compound product,molecules
R1726 T2673 T2671 pobj molecules,of
R1727 T2674 T2675 det a,threshold
R1728 T2675 T2667 dobj threshold,reaches
R1729 T2676 T2675 amod chosen,threshold
R1730 T2677 T2678 punct [,15
R1731 T2678 T2646 parataxis 15,is
R1732 T2679 T2678 punct ],15
R1733 T2680 T2629 punct .,quantified
R1734 T2682 T2683 nsubjpass Fidelity,obtained
R1735 T2684 T2682 punct (,Fidelity
R1736 T2685 T2682 appos F,Fidelity
R1737 T2686 T2682 punct ),Fidelity
R1738 T2687 T2683 auxpass was,obtained
R1739 T2688 T2683 prep from,obtained
R1740 T2689 T2690 det the,slope
R1741 T2690 T2688 pobj slope,from
R1742 T2691 T2690 punct (,slope
R1743 T2692 T2690 appos S,slope
R1744 T2693 T2690 punct ),slope
R1745 T2694 T2690 prep of,slope
R1746 T2695 T2696 det the,phase
R1747 T2696 T2694 pobj phase,of
R1748 T2697 T2698 npadvmod log,linear
R1749 T2698 T2696 amod linear,phase
R1750 T2699 T2698 punct -,linear
R1751 T2700 T2696 prep of,phase
R1752 T2701 T2702 det the,curve
R1753 T2702 T2700 pobj curve,of
R1754 T2703 T2702 compound growth,curve
R1755 T2704 T2683 prep via,obtained
R1756 T2705 T2706 det a,polynomial
R1757 T2706 T2704 pobj polynomial,via
R1758 T2707 T2708 amod best,fit
R1759 T2708 T2706 nmod fit,polynomial
R1760 T2709 T2708 punct -,fit
R1761 T2710 T2711 amod fifth,order
R1762 T2711 T2706 compound order,polynomial
R1763 T2712 T2711 punct -,order
R1764 T2713 T2706 punct : ,polynomial
R1765 T2714 T2715 nsubj F,7.39
R1766 T2715 T2706 appos 7.39,polynomial
R1767 T2716 T2715 punct =,7.39
R1768 T2717 T2715 punct +,7.39
R1769 T2718 T2719 nummod 3.80,S
R1770 T2719 T2715 appos S,7.39
R1771 T2720 T2719 punct ×,S
R1772 T2721 T2715 punct +,7.39
R1773 T2722 T2723 nummod 1.05,S2
R1774 T2723 T2715 appos S2,7.39
R1775 T2724 T2723 punct ×,S2
R1776 T2725 T2715 punct +,7.39
R1777 T2726 T2727 nummod 0.15,S3
R1778 T2727 T2715 appos S3,7.39
R1779 T2728 T2727 punct ×,S3
R1780 T2729 T2715 punct +,7.39
R1781 T2730 T2731 quantmod 11.38,3
R1782 T2731 T2735 nummod 3,S4
R1783 T2732 T2731 punct ×,3
R1784 T2733 T2731 compound 10,3
R1785 T2734 T2731 punct -,3
R1786 T2735 T2715 appos S4,7.39
R1787 T2736 T2735 punct *,S4
R1788 T2737 T2715 punct +,7.39
R1789 T2738 T2739 quantmod 3.39,4
R1790 T2739 T2743 nummod 4,S5
R1791 T2740 T2739 punct ×,4
R1792 T2741 T2739 compound 10,4
R1793 T2742 T2739 punct -,4
R1794 T2743 T2715 appos S5,7.39
R1795 T2744 T2743 punct ×,S5
R1796 T2745 T2683 punct .,obtained
R1797 T2747 T2748 det The,number
R1798 T2748 T2749 nsubjpass number,determined
R1799 T2750 T2748 prep of,number
R1800 T2751 T2752 compound product,molecules
R1801 T2752 T2750 pobj molecules,of
R1802 T2753 T2752 prep at,molecules
R1803 T2754 T2753 pobj threshold,at
R1804 T2755 T2752 punct (,molecules
R1805 T2756 T2752 appos PCt,molecules
R1806 T2757 T2752 punct ),molecules
R1807 T2758 T2749 auxpass was,determined
R1808 T2759 T2749 prep by,determined
R1809 T2760 T2759 pobj amplification,by
R1810 T2761 T2760 prep of,amplification
R1811 T2762 T2763 amod known,amounts
R1812 T2763 T2761 pobj amounts,of
R1813 T2764 T2763 prep of,amounts
R1814 T2765 T2766 compound 18S,rRNA
R1815 T2766 T2764 pobj rRNA,of
R1816 T2767 T2760 prep according,amplification
R1817 T2768 T2767 prep to,according
R1818 T2769 T2770 nsubj PCt,T
R1819 T2770 T2768 pcomp T,to
R1820 T2771 T2770 punct =,T
R1821 T2772 T2770 punct ×,T
R1822 T2773 T2774 nmod F,Ct
R1823 T2774 T2770 appos Ct,T
R1824 T2775 T2774 punct ^,Ct
R1825 T2776 T2749 punct .,determined
R1826 T2778 T2779 nsubjpass Quantifications,used
R1827 T2780 T2778 prep of,Quantifications
R1828 T2781 T2782 compound 18S,rRNA
R1829 T2782 T2780 pobj rRNA,of
R1830 T2783 T2779 auxpass were,used
R1831 T2784 T2785 aux to,compare
R1832 T2785 T2779 advcl compare,used
R1833 T2786 T2787 compound tissue,amounts
R1834 T2787 T2785 dobj amounts,compare
R1835 T2788 T2779 punct .,used
R1836 T2790 T2791 amod Genomic,contamination
R1837 T2791 T2792 nsubjpass contamination,assessed
R1838 T2793 T2791 prep of,contamination
R1839 T2794 T2795 amod inner,ear
R1840 T2795 T2796 compound ear,samples
R1841 T2796 T2793 pobj samples,of
R1842 T2797 T2792 auxpass was,assessed
R1843 T2798 T2799 aux to,be
R1844 T2799 T2792 xcomp be,assessed
R1845 T2800 T2801 punct <,%
R1846 T2801 T2799 acomp %,be
R1847 T2802 T2801 nummod 0.02,%
R1848 T2803 T2792 prep by,assessed
R1849 T2804 T2803 pobj omission,by
R1850 T2805 T2804 prep of,omission
R1851 T2806 T2807 det the,step
R1852 T2807 T2805 pobj step,of
R1853 T2808 T2807 compound RT,step
R1854 T2809 T2792 punct .,assessed
R1855 T2833 T2834 advmod In,vitro
R1856 T2834 T2835 amod vitro,electrophysiology
R1857 T2837 T2838 nsubjpass Animals,anesthetized
R1858 T2839 T2838 auxpass were,anesthetized
R1859 T2840 T2838 advmod deeply,anesthetized
R1860 T2841 T2838 prep with,anesthetized
R1861 T2842 T2843 compound sodium,pentobarbital
R1862 T2843 T2841 pobj pentobarbital,with
R1863 T2844 T2845 punct (,mg
R1864 T2845 T2838 parataxis mg,anesthetized
R1865 T2846 T2845 nummod 100,mg
R1866 T2847 T2848 punct /,kg
R1867 T2848 T2845 prep kg,mg
R1868 T2849 T2845 advmod i.p.,mg
R1869 T2850 T2845 punct ),mg
R1870 T2851 T2838 punct .,anesthetized
R1871 T2853 T2854 compound Stria,vascularis
R1872 T2854 T2855 nsubjpass vascularis,obtained
R1873 T2856 T2854 prep without,vascularis
R1874 T2857 T2858 amod spiral,ligament
R1875 T2858 T2856 pobj ligament,without
R1876 T2859 T2855 auxpass was,obtained
R1877 T2860 T2855 prep by,obtained
R1878 T2861 T2860 pobj microdissection,by
R1879 T2862 T2855 punct .,obtained
R1880 T2864 T2865 nsubjpass Currents,recorded
R1881 T2866 T2864 acl generated,Currents
R1882 T2867 T2866 agent by,generated
R1883 T2868 T2869 det the,epithelium
R1884 T2869 T2867 pobj epithelium,by
R1885 T2870 T2869 nmod stria,epithelium
R1886 T2871 T2869 amod marginal,epithelium
R1887 T2872 T2865 auxpass were,recorded
R1888 T2873 T2874 punct [,16
R1889 T2874 T2865 parataxis 16,recorded
R1890 T2875 T2874 punct ],16
R1891 T2876 T2865 punct .,recorded
R1892 T2878 T2879 det A,microelectrode
R1893 T2879 T2884 nsubjpass microelectrode,positioned
R1894 T2880 T2881 compound Pt,Ir
R1895 T2881 T2879 compound Ir,microelectrode
R1896 T2882 T2881 punct -,Ir
R1897 T2883 T2879 compound wire,microelectrode
R1898 T2885 T2879 prep with,microelectrode
R1899 T2886 T2887 det a,tip
R1900 T2887 T2885 pobj tip,with
R1901 T2888 T2887 nmod Pt,tip
R1902 T2889 T2888 punct -,Pt
R1903 T2890 T2888 amod black,Pt
R1904 T2891 T2884 auxpass was,positioned
R1905 T2892 T2893 compound 20,30
R1906 T2893 T2895 nummod 30,μm
R1907 T2894 T2893 punct –,30
R1908 T2895 T2896 npadvmod μm,from
R1909 T2896 T2884 prep from,positioned
R1910 T2897 T2898 det the,surface
R1911 T2898 T2896 pobj surface,from
R1912 T2899 T2898 amod apical,surface
R1913 T2900 T2898 prep of,surface
R1914 T2901 T2902 det the,epithelium
R1915 T2902 T2900 pobj epithelium,of
R1916 T2903 T2884 cc and,positioned
R1917 T2904 T2884 conj vibrated,positioned
R1918 T2905 T2904 prep at,vibrated
R1919 T2906 T2907 compound 200,400
R1920 T2907 T2909 nummod 400,Hz
R1921 T2908 T2907 punct –,400
R1922 T2909 T2905 pobj Hz,at
R1923 T2910 T2904 agent by,vibrated
R1924 T2911 T2912 amod piezo,electric
R1925 T2912 T2914 amod electric,elements
R1926 T2913 T2912 punct -,electric
R1927 T2914 T2910 pobj elements,by
R1928 T2915 T2914 compound bimorph,elements
R1929 T2916 T2917 punct (,version
R1930 T2917 T2914 parataxis version,elements
R1931 T2918 T2919 compound Applicable,Electronics
R1932 T2919 T2917 dep Electronics,version
R1933 T2920 T2919 punct ", ",Electronics
R1934 T2921 T2922 compound Forest,Dale
R1935 T2922 T2919 npadvmod Dale,Electronics
R1936 T2923 T2919 punct ", ",Electronics
R1937 T2924 T2919 npadvmod MA,Electronics
R1938 T2925 T2917 punct ;,version
R1939 T2926 T2917 compound ASET,version
R1940 T2927 T2917 nummod 1.05,version
R1941 T2928 T2917 punct ", ",version
R1942 T2929 T2930 compound Science,Wares
R1943 T2930 T2917 npadvmod Wares,version
R1944 T2931 T2917 punct ", ",version
R1945 T2932 T2933 compound East,Falmouth
R1946 T2933 T2917 npadvmod Falmouth,version
R1947 T2934 T2917 punct ", ",version
R1948 T2935 T2917 npadvmod MA,version
R1949 T2936 T2917 punct ),version
R1950 T2937 T2884 punct .,positioned
R1951 T2939 T2940 det A,electrode
R1952 T2940 T2944 nsubj electrode,served
R1953 T2941 T2940 nmod Pt,electrode
R1954 T2942 T2941 punct -,Pt
R1955 T2943 T2941 amod black,Pt
R1956 T2945 T2946 punct (,gauge
R1957 T2946 T2940 parataxis gauge,electrode
R1958 T2947 T2946 nummod 26,gauge
R1959 T2948 T2946 punct -,gauge
R1960 T2949 T2946 punct ),gauge
R1961 T2950 T2944 prep as,served
R1962 T2951 T2950 pobj reference,as
R1963 T2952 T2944 prep in,served
R1964 T2953 T2954 det the,chamber
R1965 T2954 T2952 pobj chamber,in
R1966 T2955 T2954 compound bath,chamber
R1967 T2956 T2944 punct .,served
R1968 T2958 T2959 det The,signals
R1969 T2959 T2960 nsubjpass signals,digitized
R1970 T2961 T2959 prep from,signals
R1971 T2962 T2963 det the,detectors
R1972 T2963 T2961 pobj detectors,from
R1973 T2964 T2965 npadvmod phase,sensitive
R1974 T2965 T2963 amod sensitive,detectors
R1975 T2966 T2965 punct -,sensitive
R1976 T2967 T2960 auxpass were,digitized
R1977 T2968 T2969 punct (,bit
R1978 T2969 T2960 parataxis bit,digitized
R1979 T2970 T2969 nummod 16,bit
R1980 T2971 T2969 punct ),bit
R1981 T2972 T2960 prep at,digitized
R1982 T2973 T2974 det a,rate
R1983 T2974 T2972 pobj rate,at
R1984 T2975 T2974 prep of,rate
R1985 T2976 T2977 nummod 0.5,Hz
R1986 T2977 T2975 pobj Hz,of
R1987 T2978 T2960 punct .,digitized
R1988 T2980 T2981 det The,output
R1989 T2981 T2982 nsubjpass output,expressed
R1990 T2983 T2982 auxpass was,expressed
R1991 T2984 T2982 prep as,expressed
R1992 T2985 T2986 amod current,density
R1993 T2986 T2984 pobj density,as
R1994 T2987 T2986 prep at,density
R1995 T2988 T2989 det the,electrode
R1996 T2989 T2987 pobj electrode,at
R1997 T2990 T2982 punct .,expressed
R1998 T3050 T3051 advmod In,situ
R1999 T3051 T3052 amod situ,electrophysiology
R2000 T3054 T3055 nsubjpass Animals,anesthetized
R2001 T3056 T3055 auxpass were,anesthetized
R2002 T3057 T3055 prep with,anesthetized
R2003 T3058 T3057 pobj inactin,with
R2004 T3059 T3060 punct (,mg
R2005 T3060 T3058 parataxis mg,inactin
R2006 T3061 T3062 compound thiobutabarbital,salt
R2007 T3062 T3060 dep salt,mg
R2008 T3063 T3062 compound sodium,salt
R2009 T3064 T3060 punct ", ",mg
R2010 T3065 T3060 nummod 140,mg
R2011 T3066 T3067 punct /,kg
R2012 T3067 T3060 prep kg,mg
R2013 T3068 T3060 advmod ip,mg
R2014 T3069 T3060 punct ),mg
R2015 T3070 T3055 punct .,anesthetized
R2016 T3072 T3073 det The,potential
R2017 T3073 T3075 nsubjpass potential,measured
R2018 T3074 T3073 amod endocochlear,potential
R2019 T3076 T3073 cc and,potential
R2020 T3077 T3078 det the,K
R2021 T3078 T3073 conj K,potential
R2022 T3079 T3078 amod endolymphatic,K
R2023 T3080 T3078 punct [,K
R2024 T3081 T3078 punct +,K
R2025 T3082 T3078 punct ],K
R2026 T3083 T3075 auxpass were,measured
R2027 T3084 T3075 prep with,measured
R2028 T3085 T3086 amod double,barreled
R2029 T3086 T3088 amod barreled,microelectrodes
R2030 T3087 T3086 punct -,barreled
R2031 T3088 T3084 pobj microelectrodes,with
R2032 T3089 T3090 punct [,17
R2033 T3090 T3075 parataxis 17,measured
R2034 T3091 T3090 punct ],17
R2035 T3092 T3075 punct .,measured
R2036 T3094 T3095 nsubjpass Measurements,made
R2037 T3096 T3095 auxpass were,made
R2038 T3097 T3095 prep in,made
R2039 T3098 T3099 det the,turn
R2040 T3099 T3097 pobj turn,in
R2041 T3100 T3099 amod basal,turn
R2042 T3101 T3095 prep by,made
R2043 T3102 T3103 det a,approach
R2044 T3103 T3101 pobj approach,by
R2045 T3104 T3105 amod round,window
R2046 T3105 T3103 compound window,approach
R2047 T3106 T3105 punct -,window
R2048 T3107 T3103 prep through,approach
R2049 T3108 T3109 det the,membrane
R2050 T3109 T3107 pobj membrane,through
R2051 T3110 T3109 amod basilar,membrane
R2052 T3111 T3107 cc and,through
R2053 T3112 T3107 conj in,through
R2054 T3113 T3114 det the,turn
R2055 T3114 T3112 pobj turn,in
R2056 T3115 T3114 amod apical,turn
R2057 T3116 T3117 mark after,thinning
R2058 T3117 T3095 advcl thinning,made
R2059 T3118 T3119 det the,bone
R2060 T3119 T3117 dobj bone,thinning
R2061 T3120 T3117 prep over,thinning
R2062 T3121 T3122 det the,vascularis
R2063 T3122 T3120 pobj vascularis,over
R2064 T3123 T3122 compound stria,vascularis
R2065 T3124 T3117 cc and,thinning
R2066 T3125 T3117 conj picking,thinning
R2067 T3126 T3127 det a,hole
R2068 T3127 T3125 dobj hole,picking
R2069 T3128 T3127 amod small,hole
R2070 T3129 T3130 punct (,μm
R2071 T3130 T3127 parataxis μm,hole
R2072 T3131 T3132 punct ~,30
R2073 T3132 T3130 nummod 30,μm
R2074 T3133 T3130 punct ),μm
R2075 T3134 T3095 punct .,made
R2076 T3136 T3137 npadvmod K,selective
R2077 T3137 T3140 amod selective,electrodes
R2078 T3138 T3136 punct +,K
R2079 T3139 T3137 punct -,selective
R2080 T3140 T3141 nsubjpass electrodes,calibrated
R2081 T3142 T3141 auxpass were,calibrated
R2082 T3143 T3141 prep in,calibrated
R2083 T3144 T3143 pobj solutions,in
R2084 T3145 T3144 prep of,solutions
R2085 T3146 T3147 amod constant,concentration
R2086 T3147 T3145 pobj concentration,of
R2087 T3148 T3147 nmod cation,concentration
R2088 T3149 T3150 punct (,K
R2089 T3150 T3148 parataxis K,cation
R2090 T3151 T3150 punct +,K
R2091 T3152 T3150 cc and,K
R2092 T3153 T3150 conj Na,K
R2093 T3154 T3153 punct +,Na
R2094 T3155 T3150 punct ),K
R2095 T3156 T3144 prep of,solutions
R2096 T3157 T3158 nummod 150,mM
R2097 T3158 T3156 pobj mM,of
R2098 T3159 T3141 punct .,calibrated
R2099 T3161 T3162 det The,selectivity
R2100 T3162 T3164 nsubj selectivity,produced
R2101 T3163 T3162 amod minor,selectivity
R2102 T3165 T3162 prep of,selectivity
R2103 T3166 T3167 det the,electrodes
R2104 T3167 T3165 pobj electrodes,of
R2105 T3168 T3167 nmod K,electrodes
R2106 T3169 T3168 punct +,K
R2107 T3170 T3162 prep for,selectivity
R2108 T3171 T3170 pobj Na,for
R2109 T3172 T3171 punct +,Na
R2110 T3173 T3174 det a,nonlinearity
R2111 T3174 T3164 dobj nonlinearity,produced
R2112 T3175 T3174 prep in,nonlinearity
R2113 T3176 T3177 det the,curve
R2114 T3177 T3175 pobj curve,in
R2115 T3178 T3177 compound calibration,curve
R2116 T3179 T3174 punct ", ",nonlinearity
R2117 T3180 T3181 dep which,fitted
R2118 T3181 T3174 relcl fitted,nonlinearity
R2119 T3182 T3181 auxpass was,fitted
R2120 T3183 T3181 advmod closely,fitted
R2121 T3184 T3181 prep by,fitted
R2122 T3185 T3186 det the,equation
R2123 T3186 T3184 pobj equation,by
R2124 T3187 T3186 compound Nicolski,equation
R2125 T3188 T3181 advcl using,fitted
R2126 T3189 T3190 amod nonlinear,software
R2127 T3190 T3188 dobj software,using
R2128 T3191 T3190 nmod curve,software
R2129 T3192 T3191 punct -,curve
R2130 T3193 T3191 amod fitting,curve
R2131 T3194 T3195 punct (,OriginLab
R2132 T3195 T3190 parataxis OriginLab,software
R2133 T3196 T3195 punct ", ",OriginLab
R2134 T3197 T3195 npadvmod Northampton,OriginLab
R2135 T3198 T3195 punct ", ",OriginLab
R2136 T3199 T3195 npadvmod MA,OriginLab
R2137 T3200 T3195 punct ),OriginLab
R2138 T3201 T3174 punct : ,nonlinearity
R2139 T3202 T3203 nsubj V,K
R2140 T3203 T3174 appos K,nonlinearity
R2141 T3204 T3203 punct =,K
R2142 T3205 T3203 nmod Vi,K
R2143 T3206 T3203 punct +,K
R2144 T3207 T3203 nmod S,K
R2145 T3208 T3203 punct ×,K
R2146 T3209 T3203 nmod log,K
R2147 T3210 T3203 punct (,K
R2148 T3211 T3203 punct [,K
R2149 T3212 T3203 punct +,K
R2150 T3213 T3203 punct ],K
R2151 T3214 T3203 punct +,K
R2152 T3215 T3216 nmod A,Na
R2153 T3216 T3203 appos Na,K
R2154 T3217 T3216 punct ×,Na
R2155 T3218 T3216 punct [,Na
R2156 T3219 T3216 punct +,Na
R2157 T3220 T3203 punct ],K
R2158 T3221 T3203 punct ),K
R2159 T3222 T3203 punct ", ",K
R2160 T3223 T3224 advmod where,is
R2161 T3224 T3203 advcl is,K
R2162 T3225 T3224 nsubj Vi,is
R2163 T3226 T3227 det an,term
R2164 T3227 T3224 attr term,is
R2165 T3228 T3227 amod offset,term
R2166 T3229 T3224 punct ", ",is
R2167 T3230 T3231 nsubj S,is
R2168 T3231 T3224 conj is,is
R2169 T3232 T3231 attr slope,is
R2170 T3233 T3231 punct ", ",is
R2171 T3234 T3235 nsubj A,is
R2172 T3235 T3231 conj is,is
R2173 T3236 T3235 attr selectivity,is
R2174 T3237 T3235 punct ", ",is
R2175 T3238 T3235 cc and,is
R2176 T3239 T3240 punct [,Na
R2177 T3240 T3241 nsubj Na,is
R2178 T3241 T3235 conj is,is
R2179 T3242 T3240 punct +,Na
R2180 T3243 T3240 punct ],Na
R2181 T3244 T3245 nmod Na,concentration
R2182 T3245 T3241 attr concentration,is
R2183 T3246 T3244 punct +,Na
R2184 T3247 T3164 punct .,produced
R2185 T3249 T3250 nsubjpass Calibrations,made
R2186 T3251 T3250 auxpass were,made
R2187 T3252 T3253 advmod immediately,after
R2188 T3253 T3250 prep after,made
R2189 T3254 T3253 pobj withdrawal,after
R2190 T3255 T3254 prep of,withdrawal
R2191 T3256 T3257 det the,electrodes
R2192 T3257 T3255 pobj electrodes,of
R2193 T3258 T3254 prep from,withdrawal
R2194 T3259 T3260 det the,cochlea
R2195 T3260 T3258 pobj cochlea,from
R2196 T3261 T3250 punct .,made
R2197 T3263 T3264 nmod Plasma,concentrations
R2198 T3264 T3267 nsubjpass concentrations,obtained
R2199 T3265 T3264 nmod K,concentrations
R2200 T3266 T3265 punct +,K
R2201 T3268 T3267 auxpass were,obtained
R2202 T3269 T3267 advcl using,obtained
R2203 T3270 T3271 det a,analyzer
R2204 T3271 T3269 dobj analyzer,using
R2205 T3272 T3271 compound blood,analyzer
R2206 T3273 T3274 punct (,M
R2207 T3274 T3267 parataxis M,obtained
R2208 T3275 T3274 compound Stat,M
R2209 T3276 T3274 compound Profile,M
R2210 T3277 T3274 punct ", ",M
R2211 T3278 T3279 compound Nova,Biomedical
R2212 T3279 T3274 npadvmod Biomedical,M
R2213 T3280 T3274 punct ", ",M
R2214 T3281 T3274 npadvmod Waltham,M
R2215 T3282 T3274 punct ", ",M
R2216 T3283 T3274 npadvmod MA,M
R2217 T3284 T3274 punct ),M
R2218 T3285 T3267 punct .,obtained
R2219 T3287 T3288 nsubjpass Data,presented
R2220 T3288 T3290 ccomp presented,denotes
R2221 T3289 T3288 auxpass are,presented
R2222 T3291 T3288 prep as,presented
R2223 T3292 T3293 compound mean,sem
R2224 T3293 T3291 pobj sem,as
R2225 T3294 T3293 compound ±,sem
R2226 T3295 T3290 punct ;,denotes
R2227 T3296 T3290 nsubj n,denotes
R2228 T3297 T3298 det the,number
R2229 T3298 T3290 dobj number,denotes
R2230 T3299 T3298 prep of,number
R2231 T3300 T3299 pobj experiments,of
R2232 T3301 T3290 punct .,denotes
R2233 T3303 T3304 nsubjpass Differences,considered
R2234 T3305 T3304 auxpass were,considered
R2235 T3306 T3304 oprd significant,considered
R2236 T3307 T3308 advmod when,0.05
R2237 T3308 T3304 advcl 0.05,considered
R2238 T3309 T3308 nsubj p,0.05
R2239 T3310 T3308 punct <,0.05
R2240 T3311 T3304 punct .,considered
R2269 T4701 T4702 advmod In,situ
R2270 T4702 T4703 amod situ,hybridization
R2271 T4703 T4704 nsubj hybridization,suggested
R2272 T4705 T4703 prep in,hybridization
R2273 T4706 T4707 det the,cochlea
R2274 T4707 T4705 pobj cochlea,in
R2275 T4708 T4704 aux has,suggested
R2276 T4709 T4710 mark that,expressed
R2277 T4710 T4704 ccomp expressed,suggested
R2278 T4711 T4712 compound pendrin,mRNA
R2279 T4712 T4710 nsubjpass mRNA,expressed
R2280 T4713 T4710 auxpass is,expressed
R2281 T4714 T4710 prep in,expressed
R2282 T4715 T4714 pobj cells,in
R2283 T4716 T4717 dep that,reside
R2284 T4717 T4715 relcl reside,cells
R2285 T4718 T4719 advmod immediately,beneath
R2286 T4719 T4717 prep beneath,reside
R2287 T4720 T4721 det the,prominence
R2288 T4721 T4719 pobj prominence,beneath
R2289 T4722 T4721 amod spiral,prominence
R2290 T4723 T4721 prep on,prominence
R2291 T4724 T4725 det the,wall
R2292 T4725 T4723 pobj wall,on
R2293 T4726 T4725 amod lateral,wall
R2294 T4727 T4725 prep of,wall
R2295 T4728 T4729 det the,sulcus
R2296 T4729 T4727 pobj sulcus,of
R2297 T4730 T4729 amod external,sulcus
R2298 T4731 T4732 punct [,8
R2299 T4732 T4704 parataxis 8,suggested
R2300 T4733 T4732 punct ],8
R2301 T4734 T4704 punct .,suggested
R2302 T4736 T4737 aux To,determine
R2303 T4737 T4738 advcl determine,performed
R2304 T4739 T4740 det the,location
R2305 T4740 T4737 dobj location,determine
R2306 T4741 T4740 prep of,location
R2307 T4742 T4743 compound pendrin,expression
R2308 T4743 T4741 pobj expression,of
R2309 T4744 T4743 compound protein,expression
R2310 T4745 T4738 punct ", ",performed
R2311 T4746 T4738 nsubj we,performed
R2312 T4747 T4748 amod confocal,immunocytochemistry
R2313 T4748 T4738 dobj immunocytochemistry,performed
R2314 T4749 T4738 prep on,performed
R2315 T4750 T4749 pobj cryosections,on
R2316 T4751 T4750 acl prepared,cryosections
R2317 T4752 T4751 prep from,prepared
R2318 T4753 T4754 amod temporal,bones
R2319 T4754 T4752 pobj bones,from
R2320 T4755 T4754 prep of,bones
R2321 T4756 T4757 amod normal,mice
R2322 T4757 T4755 pobj mice,of
R2323 T4758 T4757 punct (,mice
R2324 T4759 T4757 nmod Slc26a4,mice
R2325 T4760 T4759 punct +,Slc26a4
R2326 T4761 T4759 punct /,Slc26a4
R2327 T4762 T4759 punct +,Slc26a4
R2328 T4763 T4757 punct ),mice
R2329 T4764 T4738 advcl using,performed
R2330 T4765 T4766 det an,antibody
R2331 T4766 T4764 dobj antibody,using
R2332 T4767 T4766 amod established,antibody
R2333 T4768 T4769 punct [,3
R2334 T4769 T4738 parataxis 3,performed
R2335 T4770 T4769 punct ],3
R2336 T4771 T4738 punct .,performed
R2337 T4773 T4774 nsubj Staining,was
R2338 T4775 T4774 acomp absent,was
R2339 T4776 T4777 advmod when,pre-absorbed
R2340 T4777 T4774 advcl pre-absorbed,was
R2341 T4778 T4779 det the,antibody
R2342 T4779 T4777 nsubjpass antibody,pre-absorbed
R2343 T4780 T4779 amod primary,antibody
R2344 T4781 T4777 auxpass was,pre-absorbed
R2345 T4782 T4777 prep with,pre-absorbed
R2346 T4783 T4784 det the,peptide
R2347 T4784 T4782 pobj peptide,with
R2348 T4785 T4784 amod antigenic,peptide
R2349 T4786 T4787 punct (,shown
R2350 T4787 T4777 parataxis shown,pre-absorbed
R2351 T4788 T4787 nsubj data,shown
R2352 T4789 T4787 neg not,shown
R2353 T4790 T4787 punct ),shown
R2354 T4791 T4774 punct .,was
R2355 T4793 T4794 amod Strong,expression
R2356 T4794 T4795 nsubjpass expression,observed
R2357 T4796 T4794 prep of,expression
R2358 T4797 T4796 pobj pendrin,of
R2359 T4798 T4795 auxpass was,observed
R2360 T4799 T4800 preconj not,in
R2361 T4800 T4795 prep in,observed
R2362 T4801 T4799 advmod only,not
R2363 T4802 T4803 amod outer,sulcus
R2364 T4803 T4804 nmod sulcus,cells
R2365 T4804 T4800 pobj cells,in
R2366 T4805 T4804 amod epithelial,cells
R2367 T4806 T4807 punct ", ",predicted
R2368 T4807 T4804 parataxis predicted,cells
R2369 T4808 T4807 mark as,predicted
R2370 T4809 T4807 prep from,predicted
R2371 T4810 T4811 advmod in,situ
R2372 T4811 T4812 amod situ,data
R2373 T4812 T4809 pobj data,from
R2374 T4813 T4812 compound hybridization,data
R2375 T4814 T4807 punct ", ",predicted
R2376 T4815 T4800 cc but,in
R2377 T4816 T4815 advmod also,but
R2378 T4817 T4800 conj in,in
R2379 T4818 T4819 compound root,cells
R2380 T4819 T4817 pobj cells,in
R2381 T4820 T4817 punct ", ",in
R2382 T4821 T4817 conj in,in
R2383 T4822 T4823 amod apical,membranes
R2384 T4823 T4821 pobj membranes,in
R2385 T4824 T4823 prep of,membranes
R2386 T4825 T4826 amod spiral,prominence
R2387 T4826 T4827 nmod prominence,cells
R2388 T4827 T4824 pobj cells,of
R2389 T4828 T4827 nmod surface,cells
R2390 T4829 T4827 amod epithelial,cells
R2391 T4830 T4821 cc and,in
R2392 T4831 T4821 conj in,in
R2393 T4832 T4833 amod apical,membranes
R2394 T4833 T4831 pobj membranes,in
R2395 T4834 T4833 prep of,membranes
R2396 T4835 T4836 npadvmod spindle,shaped
R2397 T4836 T4838 amod shaped,cells
R2398 T4837 T4836 punct -,shaped
R2399 T4838 T4834 pobj cells,of
R2400 T4839 T4840 dep that,are
R2401 T4840 T4838 relcl are,cells
R2402 T4841 T4840 attr part,are
R2403 T4842 T4841 prep of,part
R2404 T4843 T4844 compound stria,vascularis
R2405 T4844 T4842 pobj vascularis,of
R2406 T4845 T4846 punct (,1c
R2407 T4846 T4795 parataxis 1c,observed
R2408 T4847 T4846 nmod Fig.,1c
R2409 T4848 T4846 nummod 1a,1c
R2410 T4849 T4846 punct ",",1c
R2411 T4850 T4846 nummod 1b,1c
R2412 T4851 T4846 punct ",",1c
R2413 T4852 T4846 punct ),1c
R2414 T4853 T4795 punct .,observed
R2415 T4855 T4856 det The,presence
R2416 T4856 T4857 nsubj presence,suggests
R2417 T4858 T4856 prep of,presence
R2418 T4859 T4858 pobj pendrin,of
R2419 T4860 T4856 prep in,presence
R2420 T4861 T4862 npadvmod spindle,shaped
R2421 T4862 T4864 amod shaped,cells
R2422 T4863 T4862 punct -,shaped
R2423 T4864 T4860 pobj cells,in
R2424 T4865 T4866 mark that,secrete
R2425 T4866 T4857 ccomp secrete,suggests
R2426 T4867 T4868 det these,cells
R2427 T4868 T4866 nsubj cells,secrete
R2428 T4869 T4866 dobj HCO3,secrete
R2429 T4870 T4869 punct -,HCO3
R2430 T4871 T4866 prep into,secrete
R2431 T4872 T4871 pobj endolymph,into
R2432 T4873 T4857 punct .,suggests
R2433 T4875 T4876 npadvmod Pendrin,mediated
R2434 T4876 T4878 amod mediated,transport
R2435 T4877 T4876 punct -,mediated
R2436 T4878 T4881 nsubjpass transport,shown
R2437 T4879 T4878 nmod HCO3,transport
R2438 T4880 T4879 punct -,HCO3
R2439 T4882 T4881 aux has,shown
R2440 T4883 T4881 advmod previously,shown
R2441 T4884 T4881 auxpass been,shown
R2442 T4885 T4881 prep in,shown
R2443 T4886 T4887 det the,kidney
R2444 T4887 T4885 pobj kidney,in
R2445 T4888 T4889 punct [,9
R2446 T4889 T4881 parataxis 9,shown
R2447 T4890 T4889 punct ],9
R2448 T4891 T4881 punct .,shown
R2449 T4893 T4894 nsubjpass HCO3,generated
R2450 T4895 T4893 punct -,HCO3
R2451 T4896 T4893 prep in,HCO3
R2452 T4897 T4898 det the,cochlea
R2453 T4898 T4896 pobj cochlea,in
R2454 T4899 T4894 aux may,generated
R2455 T4900 T4894 auxpass be,generated
R2456 T4901 T4894 prep from,generated
R2457 T4902 T4901 pobj CO2,from
R2458 T4903 T4894 prep by,generated
R2459 T4904 T4905 amod carbonic,anhydrase
R2460 T4905 T4903 pobj anhydrase,by
R2461 T4906 T4905 acl located,anhydrase
R2462 T4907 T4906 prep in,located
R2463 T4908 T4909 amod strial,cells
R2464 T4909 T4907 pobj cells,in
R2465 T4910 T4909 amod intermediate,cells
R2466 T4911 T4912 punct [,19
R2467 T4912 T4894 parataxis 19,generated
R2468 T4913 T4912 nummod 18,19
R2469 T4914 T4912 punct ",",19
R2470 T4915 T4912 punct ],19
R2471 T4916 T4894 punct .,generated
R2472 T4918 T4919 nsubjpass CO2,supplied
R2473 T4920 T4919 aux may,supplied
R2474 T4921 T4919 auxpass be,supplied
R2475 T4922 T4919 agent by,supplied
R2476 T4923 T4924 det the,cells
R2477 T4924 T4922 pobj cells,by
R2478 T4925 T4926 advmod metabolically,active
R2479 T4926 T4924 amod active,cells
R2480 T4927 T4926 advmod highly,active
R2481 T4928 T4929 npadvmod stria,marginal
R2482 T4929 T4924 amod marginal,cells
R2483 T4930 T4919 punct .,supplied
R2484 T4932 T4933 nsubj It,is
R2485 T4934 T4933 acomp conceivable,is
R2486 T4935 T4936 mark that,interrupts
R2487 T4936 T4933 ccomp interrupts,is
R2488 T4937 T4938 compound pendrin,dysfunction
R2489 T4938 T4936 nsubj dysfunction,interrupts
R2490 T4939 T4940 nmod HCO3,secretion
R2491 T4940 T4936 dobj secretion,interrupts
R2492 T4941 T4939 punct -,HCO3
R2493 T4942 T4936 cc and,interrupts
R2494 T4943 T4936 conj leads,interrupts
R2495 T4944 T4943 prep to,leads
R2496 T4945 T4946 det an,accumulation
R2497 T4946 T4944 pobj accumulation,to
R2498 T4947 T4946 prep of,accumulation
R2499 T4948 T4947 pobj HCO3,of
R2500 T4949 T4948 punct -,HCO3
R2501 T4950 T4943 prep in,leads
R2502 T4951 T4952 compound stria,vascularis
R2503 T4952 T4950 pobj vascularis,in
R2504 T4953 T4933 punct .,is
R2505 T4955 T4956 amod Preliminary,data
R2506 T4956 T4957 nsubj data,support
R2507 T4958 T4959 punct (,Wangemann
R2508 T4959 T4956 meta Wangemann,data
R2509 T4960 T4959 nmod et,Wangemann
R2510 T4961 T4959 nmod al.,Wangemann
R2511 T4962 T4959 punct ", ",Wangemann
R2512 T4963 T4959 amod unpublished,Wangemann
R2513 T4964 T4959 punct ),Wangemann
R2514 T4965 T4966 det a,role
R2515 T4966 T4957 dobj role,support
R2516 T4967 T4966 prep for,role
R2517 T4968 T4967 pobj pendrin,for
R2518 T4969 T4966 prep in,role
R2519 T4970 T4971 nmod HCO3,secretion
R2520 T4971 T4969 pobj secretion,in
R2521 T4972 T4970 punct -,HCO3
R2522 T4973 T4971 prep into,secretion
R2523 T4974 T4973 pobj endolymph,into
R2524 T4975 T4957 punct .,support
R2525 T4982 T4983 nsubjpass xtent,determined
R2526 T4984 T4982 prep of,xtent
R2527 T4985 T4986 compound pendrin,expression
R2528 T4986 T4984 pobj expression,of
R2529 T4987 T4982 prep in,xtent
R2530 T4988 T4989 amod spiral,cells
R2531 T4989 T4987 pobj cells,in
R2532 T4990 T4989 compound prominence,cells
R2533 T4991 T4989 cc and,cells
R2534 T4992 T4993 compound stria,vascularis
R2535 T4993 T4989 conj vascularis,cells
R2536 T4994 T4983 auxpass was,determined
R2537 T4995 T4983 prep by,determined
R2538 T4996 T4995 pcomp labeling,by
R2539 T4997 T4996 dobj KCNQ1,labeling
R2540 T4998 T4997 punct ", ",KCNQ1
R2541 T4999 T5000 det a,channel
R2542 T5000 T4997 appos channel,KCNQ1
R2543 T5001 T5000 nmod K,channel
R2544 T5002 T5001 punct +,K
R2545 T5003 T5004 dep that,expressed
R2546 T5004 T5000 relcl expressed,channel
R2547 T5005 T5004 auxpass is,expressed
R2548 T5006 T5004 prep in,expressed
R2549 T5007 T5008 amod strial,marginal
R2550 T5008 T5009 amod marginal,cells
R2551 T5009 T5006 pobj cells,in
R2552 T5010 T4997 punct ", ",KCNQ1
R2553 T5011 T4997 cc and,KCNQ1
R2554 T5012 T4997 conj ZO,KCNQ1
R2555 T5013 T5012 punct -,ZO
R2556 T5014 T5012 nummod 1,ZO
R2557 T5015 T5012 punct ", ",ZO
R2558 T5016 T5017 det a,protein
R2559 T5017 T5012 appos protein,ZO
R2560 T5018 T5019 amod tight,junction
R2561 T5019 T5017 compound junction,protein
R2562 T5020 T5021 dep that,labels
R2563 T5021 T5017 relcl labels,protein
R2564 T5022 T5023 amod basal,cells
R2565 T5023 T5021 dobj cells,labels
R2566 T5024 T5021 cc and,labels
R2567 T5025 T5026 advmod thereby,delineates
R2568 T5026 T5021 conj delineates,labels
R2569 T5027 T5028 det the,boundaries
R2570 T5028 T5026 dobj boundaries,delineates
R2571 T5029 T5028 prep of,boundaries
R2572 T5030 T5031 compound stria,vascularis
R2573 T5031 T5029 pobj vascularis,of
R2574 T5032 T4983 punct .,determined
R2575 T5034 T5035 amod Dual,experiments
R2576 T5035 T5037 nsubj experiments,demonstrated
R2577 T5036 T5035 compound labeling,experiments
R2578 T5038 T5039 mark that,expressed
R2579 T5039 T5037 ccomp expressed,demonstrated
R2580 T5040 T5039 nsubjpass pendrin,expressed
R2581 T5041 T5039 auxpass is,expressed
R2582 T5042 T5039 prep in,expressed
R2583 T5043 T5044 npadvmod spindle,shaped
R2584 T5044 T5046 amod shaped,cells
R2585 T5045 T5044 punct -,shaped
R2586 T5046 T5042 pobj cells,in
R2587 T5047 T5046 punct ", ",cells
R2588 T5048 T5049 dep which,are
R2589 T5049 T5046 relcl are,cells
R2590 T5050 T5051 amod surface,cells
R2591 T5051 T5049 attr cells,are
R2592 T5052 T5051 amod epithelial,cells
R2593 T5053 T5051 prep in,cells
R2594 T5054 T5055 compound stria,vascularis
R2595 T5055 T5053 pobj vascularis,in
R2596 T5056 T5051 amod adjacent,cells
R2597 T5057 T5056 prep to,adjacent
R2598 T5058 T5059 amod strial,cells
R2599 T5059 T5057 pobj cells,to
R2600 T5060 T5059 amod marginal,cells
R2601 T5061 T5059 prep near,cells
R2602 T5062 T5063 det the,borders
R2603 T5063 T5061 pobj borders,near
R2604 T5064 T5063 prep of,borders
R2605 T5065 T5066 preconj both,prominence
R2606 T5066 T5064 pobj prominence,of
R2607 T5067 T5066 det the,prominence
R2608 T5068 T5066 amod spiral,prominence
R2609 T5069 T5066 cc and,prominence
R2610 T5070 T5071 poss Reissner,membrane
R2611 T5071 T5066 conj membrane,prominence
R2612 T5072 T5070 case 's,Reissner
R2613 T5073 T5074 punct (,Fig.
R2614 T5074 T5039 parataxis Fig.,expressed
R2615 T5075 T5074 nummod 1c,Fig.
R2616 T5076 T5074 punct ),Fig.
R2617 T5077 T5037 punct .,demonstrated
R2618 T5079 T5080 advmod In,situ
R2619 T5080 T5081 amod situ,hybridization
R2620 T5081 T5082 nsubj hybridization,suggested
R2621 T5083 T5081 prep in,hybridization
R2622 T5084 T5085 det the,labyrinth
R2623 T5085 T5083 pobj labyrinth,in
R2624 T5086 T5085 amod vestibular,labyrinth
R2625 T5087 T5088 mark that,expressed
R2626 T5088 T5082 ccomp expressed,suggested
R2627 T5089 T5090 compound pendrin,mRNA
R2628 T5090 T5088 nsubjpass mRNA,expressed
R2629 T5091 T5088 auxpass is,expressed
R2630 T5092 T5088 prep in,expressed
R2631 T5093 T5094 amod non-sensory,cells
R2632 T5094 T5092 pobj cells,in
R2633 T5095 T5096 punct [,8
R2634 T5096 T5082 parataxis 8,suggested
R2635 T5097 T5096 punct ],8
R2636 T5098 T5082 punct .,suggested
R2637 T5100 T5101 advcl Using,observed
R2638 T5102 T5103 amod confocal,immunocytochemistry
R2639 T5103 T5100 dobj immunocytochemistry,Using
R2640 T5104 T5100 prep on,Using
R2641 T5105 T5104 pobj cryosections,on
R2642 T5106 T5101 punct ", ",observed
R2643 T5107 T5101 nsubj we,observed
R2644 T5108 T5109 amod strong,expression
R2645 T5109 T5101 dobj expression,observed
R2646 T5110 T5109 prep of,expression
R2647 T5111 T5112 det the,protein
R2648 T5112 T5110 pobj protein,of
R2649 T5113 T5112 compound pendrin,protein
R2650 T5114 T5101 prep in,observed
R2651 T5115 T5116 det the,membrane
R2652 T5116 T5114 pobj membrane,in
R2653 T5117 T5116 amod apical,membrane
R2654 T5118 T5116 prep of,membrane
R2655 T5119 T5120 amod vestibular,cells
R2656 T5120 T5118 pobj cells,of
R2657 T5121 T5120 amod transitional,cells
R2658 T5122 T5120 prep in,cells
R2659 T5123 T5124 det the,utricle
R2660 T5124 T5122 pobj utricle,in
R2661 T5125 T5124 cc and,utricle
R2662 T5126 T5124 conj ampullae,utricle
R2663 T5127 T5128 punct (,1i
R2664 T5128 T5101 parataxis 1i,observed
R2665 T5129 T5128 nmod Fig.,1i
R2666 T5130 T5128 nummod 1g,1i
R2667 T5131 T5128 punct ",",1i
R2668 T5132 T5128 nummod 1h,1i
R2669 T5133 T5128 punct ",",1i
R2670 T5140 T5138 prep with,labeling
R2671 T5134 T5128 punct ),1i
R2672 T5141 T5140 pobj KCNQ1,with
R2673 T5142 T5143 mark that,limited
R2674 T5135 T5101 punct .,observed
R2675 T5143 T5139 ccomp limited,demonstrated
R2676 T5144 T5145 compound pendrin,expression
R2677 T5145 T5143 nsubjpass expression,limited
R2678 T5137 T5138 amod Dual,labeling
R2679 T5146 T5143 auxpass was,limited
R2680 T5147 T5143 advmod clearly,limited
R2681 T5148 T5143 prep to,limited
R2682 T5138 T5139 nsubj labeling,demonstrated
R2683 T5149 T5150 amod vestibular,cells
R2684 T5150 T5148 pobj cells,to
R2685 T5151 T5150 amod transitional,cells
R2686 T5245 T5238 xcomp develop,start
R2687 T5152 T5143 cc and,limited
R2688 T5153 T5154 aux did,extend
R2689 T5154 T5143 conj extend,limited
R2690 T5155 T5154 neg not,extend
R2691 T5156 T5154 prep to,extend
R2692 T5246 T5247 det an,space
R2693 T5157 T5158 amod other,cells
R2694 T5158 T5156 pobj cells,to
R2695 T5159 T5158 amod non-sensory,cells
R2696 T5247 T5245 dobj space,develop
R2697 T5160 T5161 amod such,as
R2698 T5161 T5158 prep as,cells
R2699 T5248 T5247 amod enlarged,space
R2700 T5162 T5163 amod vestibular,cells
R2701 T5163 T5161 pobj cells,as
R2702 T5164 T5163 amod dark,cells
R2703 T5249 T5247 amod endolymphatic,space
R2704 T5165 T5163 punct ", ",cells
R2705 T5166 T5167 dep which,identified
R2706 T5250 T5251 dep that,persists
R2707 T5167 T5163 relcl identified,cells
R2708 T5168 T5167 auxpass were,identified
R2709 T5169 T5167 advmod clearly,identified
R2710 T5251 T5247 relcl persists,space
R2711 T5170 T5167 prep by,identified
R2712 T5171 T5172 det the,expression
R2713 T5172 T5170 pobj expression,by
R2714 T5173 T5172 prep of,expression
R2715 T5174 T5173 pobj KCNQ1,of
R2716 T5175 T5174 cc and,KCNQ1
R2717 T5252 T5251 prep into,persists
R2718 T5176 T5174 conj KCNE1,KCNQ1
R2719 T5177 T5172 prep in,expression
R2720 T5178 T5179 poss their,membranes
R2721 T5253 T5252 pobj adulthood,into
R2722 T5179 T5177 pobj membranes,in
R2723 T5180 T5179 amod apical,membranes
R2724 T5181 T5139 punct .,demonstrated
R2725 T5254 T5255 punct [,14
R2726 T5183 T5184 det The,onset
R2727 T5184 T5185 nsubjpass onset,determined
R2728 T5255 T5245 parataxis 14,develop
R2729 T5186 T5184 prep of,onset
R2730 T5256 T5255 punct ],14
R2731 T5187 T5188 compound pendrin,expression
R2732 T5188 T5186 pobj expression,of
R2733 T5189 T5184 prep during,onset
R2734 T5257 T5214 punct .,become
R2735 T5190 T5189 pobj development,during
R2736 T5191 T5190 prep of,development
R2737 T5192 T5193 det the,ear
R2738 T5259 T5260 advmod Interestingly,appear
R2739 T5261 T5260 punct ", ",appear
R2740 T5193 T5191 pobj ear,of
R2741 T5194 T5193 nmod mouse,ear
R2742 T5195 T5193 amod inner,ear
R2743 T5262 T5263 amod sensory,cells
R2744 T5196 T5185 aux has,determined
R2745 T5197 T5185 auxpass been,determined
R2746 T5198 T5199 aux to,be
R2747 T5263 T5260 nsubj cells,appear
R2748 T5199 T5185 xcomp be,determined
R2749 T5200 T5201 amod embryonic,day
R2750 T5264 T5263 compound hair,cells
R2751 T5201 T5199 attr day,be
R2752 T5202 T5203 punct (,ED
R2753 T5265 T5263 prep in,cells
R2754 T5203 T5201 appos ED,day
R2755 T5204 T5203 punct ),ED
R2756 T5205 T5201 nummod 13,day
R2757 T5266 T5267 det the,cochlea
R2758 T5206 T5207 punct [,14
R2759 T5207 T5185 parataxis 14,determined
R2760 T5208 T5207 punct ],14
R2761 T5209 T5185 punct .,determined
R2762 T5267 T5265 pobj cochlea,in
R2763 T5211 T5212 advmod Morphologically,detectable
R2764 T5212 T5213 amod detectable,differences
R2765 T5213 T5214 nsubj differences,become
R2766 T5268 T5260 oprd normal,appear
R2767 T5215 T5213 prep in,differences
R2768 T5216 T5217 det the,ears
R2769 T5269 T5260 prep until,appear
R2770 T5217 T5215 pobj ears,in
R2771 T5218 T5217 amod inner,ears
R2772 T5219 T5217 prep of,ears
R2773 T5270 T5271 amod postnatal,day
R2774 T5220 T5221 nmod Slc26a4,mice
R2775 T5221 T5219 pobj mice,of
R2776 T5222 T5220 punct +,Slc26a4
R2777 T5271 T5269 pobj day,until
R2778 T5223 T5220 punct /,Slc26a4
R2779 T5224 T5220 punct +,Slc26a4
R2780 T5225 T5220 cc and,Slc26a4
R2781 T5272 T5273 punct (,PD
R2782 T5226 T5220 conj Slc26a4,Slc26a4
R2783 T5227 T5226 punct -,Slc26a4
R2784 T5228 T5226 punct /,Slc26a4
R2785 T5273 T5271 appos PD,day
R2786 T5229 T5226 punct -,Slc26a4
R2787 T5230 T5214 acomp evident,become
R2788 T5231 T5232 advmod as,early
R2789 T5274 T5273 punct ),PD
R2790 T5232 T5214 advmod early,become
R2791 T5233 T5232 prep as,early
R2792 T5234 T5233 pobj ED,as
R2793 T5275 T5271 nummod 7,day
R2794 T5235 T5234 nummod 15,ED
R2795 T5236 T5234 punct ", ",ED
R2796 T5276 T5260 cc but,appear
R2797 T5237 T5238 advmod when,start
R2798 T5238 T5234 advcl start,ED
R2799 T5239 T5240 nmod Slc26a4,mice
R2800 T5277 T5260 conj show,appear
R2801 T5240 T5238 nsubj mice,start
R2802 T5241 T5239 punct -,Slc26a4
R2803 T5278 T5279 amod clear,evidence
R2804 T5242 T5239 punct /,Slc26a4
R2805 T5243 T5239 punct -,Slc26a4
R2806 T5244 T5245 aux to,develop
R2807 T5279 T5277 dobj evidence,show
R2808 T5280 T5279 prep of,evidence
R2809 T5281 T5280 pobj degeneration,of
R2810 T5282 T5277 prep by,show
R2811 T5351 T5343 prep until,persist
R2812 T5283 T5282 pobj PD,by
R2813 T5352 T5351 pobj PD,until
R2814 T5353 T5352 nummod 7,PD
R2815 T5354 T5321 punct .,established
R2816 T5356 T5357 det The,period
R2817 T5284 T5283 nummod 15,PD
R2818 T5357 T5359 nsubj period,is
R2819 T5358 T5357 compound time,period
R2820 T5360 T5357 prep between,period
R2821 T5285 T5286 punct [,14
R2822 T5361 T5362 nmod PD,7
R2823 T5362 T5360 pobj 7,between
R2824 T5286 T5277 parataxis 14,show
R2825 T5363 T5362 cc and,7
R2826 T5364 T5362 conj 15,7
R2827 T5287 T5286 punct ],14
R2828 T5365 T5366 det the,time
R2829 T5288 T5260 punct .,appear
R2830 T5366 T5359 attr time,is
R2831 T5367 T5368 advmod when,develops
R2832 T5290 T5291 det These,observations
R2833 T5368 T5366 relcl develops,time
R2834 T5369 T5370 det the,potential
R2835 T5370 T5368 nsubj potential,develops
R2836 T5371 T5370 amod endocochlear,potential
R2837 T5291 T5292 nsubj observations,suggest
R2838 T5372 T5368 prep at,develops
R2839 T5373 T5374 det the,onset
R2840 T5293 T5294 mark that,supports
R2841 T5374 T5372 pobj onset,at
R2842 T5375 T5374 prep of,onset
R2843 T5376 T5375 pobj hearing,of
R2844 T5294 T5292 ccomp supports,suggest
R2845 T5377 T5378 punct [,22
R2846 T5378 T5368 parataxis 22,develops
R2847 T5379 T5378 punct ],22
R2848 T5295 T5296 det the,environment
R2849 T5296 T5294 nsubj environment,supports
R2850 T5380 T5359 punct .,is
R2851 T5297 T5296 amod cochlear,environment
R2852 T5382 T5383 nsubj We,hypothesized
R2853 T5384 T5385 mark that,account
R2854 T5298 T5299 det the,survival
R2855 T5385 T5383 ccomp account,hypothesized
R2856 T5386 T5387 det a,lack
R2857 T5299 T5294 dobj survival,supports
R2858 T5387 T5385 nsubj lack,account
R2859 T5388 T5387 prep of,lack
R2860 T5389 T5390 det a,potential
R2861 T5390 T5388 pobj potential,of
R2862 T5300 T5299 prep of,survival
R2863 T5391 T5390 amod normal,potential
R2864 T5392 T5390 amod endocochlear,potential
R2865 T5393 T5387 cc or,lack
R2866 T5394 T5395 det an,alteration
R2867 T5395 T5387 conj alteration,lack
R2868 T5396 T5395 prep of,alteration
R2869 T5301 T5302 amod sensory,cells
R2870 T5397 T5398 det the,concentration
R2871 T5398 T5396 pobj concentration,of
R2872 T5399 T5398 amod endolymphatic,concentration
R2873 T5302 T5300 pobj cells,of
R2874 T5400 T5398 nmod K,concentration
R2875 T5401 T5400 punct +,K
R2876 T5303 T5302 compound hair,cells
R2877 T5402 T5385 aux could,account
R2878 T5403 T5385 prep for,account
R2879 T5404 T5403 pobj deafness,for
R2880 T5304 T5294 prep in,supports
R2881 T5405 T5385 prep in,account
R2882 T5406 T5407 nmod Slc26a4,mice
R2883 T5305 T5304 pobj spite,in
R2884 T5407 T5405 pobj mice,in
R2885 T5408 T5406 punct -,Slc26a4
R2886 T5409 T5406 punct /,Slc26a4
R2887 T5306 T5305 prep of,spite
R2888 T5410 T5406 punct -,Slc26a4
R2889 T5411 T5383 punct .,hypothesized
R2890 T5307 T5308 det the,enlargement
R2891 T5413 T5414 nsubj Measurements,revealed
R2892 T5308 T5306 pobj enlargement,of
R2893 T5415 T5416 mark that,was
R2894 T5416 T5414 advcl was,revealed
R2895 T5417 T5418 det the,potential
R2896 T5309 T5308 prep of,enlargement
R2897 T5418 T5416 nsubj potential,was
R2898 T5419 T5418 amod endocochlear,potential
R2899 T5420 T5416 acomp absent,was
R2900 T5310 T5311 det the,duct
R2901 T5421 T5416 cc but,was
R2902 T5422 T5423 mark that,was
R2903 T5311 T5309 pobj duct,of
R2904 T5423 T5416 conj was,was
R2905 T5424 T5425 det the,concentration
R2906 T5425 T5423 nsubj concentration,was
R2907 T5312 T5311 amod endolymphatic,duct
R2908 T5426 T5425 amod endolymphatic,concentration
R2909 T5427 T5425 nmod K,concentration
R2910 T5428 T5427 punct +,K
R2911 T5313 T5292 punct .,suggest
R2912 T5429 T5423 acomp normal,was
R2913 T5430 T5423 prep in,was
R2914 T5315 T5316 det A,concentration
R2915 T5431 T5432 amod adult,Slc26a4
R2916 T5432 T5430 pobj Slc26a4,in
R2917 T5433 T5432 punct -,Slc26a4
R2918 T5434 T5432 punct /,Slc26a4
R2919 T5435 T5432 punct -,Slc26a4
R2920 T5436 T5437 punct (,Fig.
R2921 T5316 T5321 nsubjpass concentration,established
R2922 T5437 T5423 parataxis Fig.,was
R2923 T5317 T5316 amod normal,concentration
R2924 T5438 T5437 nummod 2a,Fig.
R2925 T5318 T5316 amod endolymphatic,concentration
R2926 T5439 T5437 punct ),Fig.
R2927 T5319 T5316 nmod K,concentration
R2928 T5440 T5414 punct .,revealed
R2929 T5442 T5443 det No,differences
R2930 T5320 T5319 punct +,K
R2931 T5443 T5445 nsubjpass differences,found
R2932 T5322 T5316 punct ", ",concentration
R2933 T5444 T5443 amod significant,differences
R2934 T5446 T5443 prep between,differences
R2935 T5447 T5448 nmod Slc26a4,mice
R2936 T5323 T5324 dep which,is
R2937 T5324 T5316 relcl is,concentration
R2938 T5325 T5324 acomp critical,is
R2939 T5326 T5325 prep for,critical
R2940 T5448 T5446 pobj mice,between
R2941 T5327 T5328 compound hair,cell
R2942 T5449 T5447 punct +,Slc26a4
R2943 T5450 T5447 punct /,Slc26a4
R2944 T5328 T5329 compound cell,survival
R2945 T5451 T5447 punct +,Slc26a4
R2946 T5452 T5447 cc and,Slc26a4
R2947 T5453 T5447 conj Slc26a4,Slc26a4
R2948 T5329 T5326 pobj survival,for
R2949 T5454 T5453 punct -,Slc26a4
R2950 T5455 T5453 punct /,Slc26a4
R2951 T5456 T5453 punct -,Slc26a4
R2952 T5330 T5331 punct [,20
R2953 T5331 T5324 parataxis 20,is
R2954 T5457 T5445 auxpass were,found
R2955 T5332 T5331 punct ],20
R2956 T5458 T5445 prep in,found
R2957 T5459 T5460 amod perilymphatic,concentrations
R2958 T5333 T5321 punct ", ",established
R2959 T5460 T5458 pobj concentrations,in
R2960 T5461 T5462 punct (,Fig.
R2961 T5334 T5321 auxpass is,established
R2962 T5462 T5459 parataxis Fig.,perilymphatic
R2963 T5463 T5462 nummod 2a,Fig.
R2964 T5464 T5462 punct ),Fig.
R2965 T5335 T5321 prep at,established
R2966 T5465 T5459 cc or,perilymphatic
R2967 T5466 T5467 compound plasma,K
R2968 T5467 T5459 conj K,perilymphatic
R2969 T5336 T5335 pobj PD,at
R2970 T5468 T5467 punct +,K
R2971 T5469 T5470 punct (,6
R2972 T5470 T5445 parataxis 6,found
R2973 T5471 T5472 dep Slc26a4,6
R2974 T5472 T5470 dep 6,6
R2975 T5473 T5471 punct +,Slc26a4
R2976 T5474 T5471 punct /,Slc26a4
R2977 T5337 T5336 nummod 3,PD
R2978 T5475 T5471 punct +,Slc26a4
R2979 T5476 T5472 punct ", ",6
R2980 T5477 T5478 quantmod 4.9,0.3
R2981 T5338 T5339 punct [,21
R2982 T5478 T5480 nummod 0.3,mM
R2983 T5479 T5478 punct ±,0.3
R2984 T5480 T5472 dep mM,6
R2985 T5339 T5321 parataxis 21,established
R2986 T5481 T5472 punct ", ",6
R2987 T5482 T5472 nsubj n,6
R2988 T5483 T5472 punct =,6
R2989 T5340 T5339 punct ],21
R2990 T5484 T5470 punct ;,6
R2991 T5485 T5470 dep Slc26a4,6
R2992 T5341 T5321 cc and,established
R2993 T5486 T5485 punct -,Slc26a4
R2994 T5487 T5485 punct /,Slc26a4
R2995 T5488 T5485 punct -,Slc26a4
R2996 T5342 T5343 aux may,persist
R2997 T5489 T5470 punct ", ",6
R2998 T5490 T5491 quantmod 5.1,0.3
R2999 T5491 T5493 nummod 0.3,mM
R3000 T5343 T5321 conj persist,established
R3001 T5492 T5491 punct ±,0.3
R3002 T5493 T5470 dep mM,6
R3003 T5494 T5470 punct ", ",6
R3004 T5344 T5343 prep in,persist
R3005 T5495 T5470 nsubj n,6
R3006 T5496 T5470 punct =,6
R3007 T5497 T5470 punct ),6
R3008 T5345 T5344 pobj Slc26a4,in
R3009 T5498 T5445 punct .,found
R3010 T5500 T5501 det These,observations
R3011 T5346 T5345 punct -,Slc26a4
R3012 T5501 T5502 nsubj observations,suggest
R3013 T5347 T5345 punct /,Slc26a4
R3014 T5503 T5504 mark that,is
R3015 T5504 T5502 ccomp is,suggest
R3016 T5505 T5506 det a,event
R3017 T5348 T5345 punct -,Slc26a4
R3018 T5506 T5504 nsubj event,is
R3019 T5507 T5506 amod primary,event
R3020 T5508 T5506 acl leading,event
R3021 T5349 T5350 advmod at,least
R3022 T5509 T5508 prep to,leading
R3023 T5510 T5509 pobj deafness,to
R3024 T5511 T5508 prep in,leading
R3025 T5512 T5513 nmod Slc26a4,mice
R3026 T5513 T5511 pobj mice,in
R3027 T5514 T5512 punct -,Slc26a4
R3028 T5350 T5351 advmod least,until
R3029 T5515 T5512 punct /,Slc26a4
R3030 T5516 T5512 punct -,Slc26a4
R3031 T5517 T5511 punct ", ",in
R3032 T5553 T5554 det The,potential
R3033 T5518 T5511 cc and,in
R3034 T5519 T5520 advmod potentially,in
R3035 T5520 T5511 conj in,in
R3036 T5521 T5520 pobj patients,in
R3037 T5522 T5521 acl suffering,patients
R3038 T5523 T5522 prep from,suffering
R3039 T5524 T5525 compound Pendred,syndrome
R3040 T5525 T5523 pobj syndrome,from
R3041 T5554 T5556 nsubjpass potential,generated
R3042 T5526 T5504 punct ", ",is
R3043 T5527 T5528 det the,loss
R3044 T5528 T5504 attr loss,is
R3045 T5529 T5528 prep of,loss
R3046 T5555 T5554 amod endocochlear,potential
R3047 T5530 T5531 det the,potential
R3048 T5531 T5529 pobj potential,of
R3049 T5532 T5531 amod endocochlear,potential
R3050 T5557 T5556 auxpass is,generated
R3051 T5533 T5502 punct .,suggest
R3052 T5535 T5536 nsubj Degeneration,is
R3053 T5558 T5556 agent by,generated
R3054 T5559 T5560 compound stria,vascularis
R3055 T5537 T5535 prep of,Degeneration
R3056 T5538 T5539 compound hair,cells
R3057 T5560 T5558 pobj vascularis,by
R3058 T5539 T5537 pobj cells,of
R3059 T5540 T5536 advmod probably,is
R3060 T5541 T5542 det a,response
R3061 T5561 T5556 prep in,generated
R3062 T5542 T5536 attr response,is
R3063 T5543 T5542 prep to,response
R3064 T5544 T5545 det the,loss
R3065 T5562 T5563 det the,wall
R3066 T5545 T5543 pobj loss,to
R3067 T5546 T5545 prep of,loss
R3068 T5563 T5561 pobj wall,in
R3069 T5547 T5548 det the,potential
R3070 T5548 T5546 pobj potential,of
R3071 T5549 T5548 amod endocochlear,potential
R3072 T5550 T5536 punct .,is
R3073 T5564 T5563 amod lateral,wall
R3074 T5565 T5563 prep of,wall
R3075 T5566 T5567 det the,cochlea
R3076 T5619 T5620 amod Marginal,cells
R3077 T5620 T5621 nsubj cells,transport
R3078 T5567 T5565 pobj cochlea,of
R3079 T5622 T5620 prep of,cells
R3080 T5623 T5624 compound stria,vascularis
R3081 T5568 T5569 punct [,23
R3082 T5624 T5622 pobj vascularis,of
R3083 T5625 T5620 punct ", ",cells
R3084 T5626 T5627 dep which,form
R3085 T5569 T5556 parataxis 23,generated
R3086 T5627 T5620 relcl form,cells
R3087 T5628 T5629 det the,barrier
R3088 T5629 T5627 dobj barrier,form
R3089 T5570 T5569 nummod 17,23
R3090 T5630 T5627 prep toward,form
R3091 T5631 T5630 pobj endolymph,toward
R3092 T5632 T5621 punct ", ",transport
R3093 T5571 T5569 punct ",",23
R3094 T5633 T5621 dobj K,transport
R3095 T5634 T5633 punct +,K
R3096 T5572 T5569 punct ],23
R3097 T5635 T5621 prep from,transport
R3098 T5636 T5637 det the,space
R3099 T5637 T5635 pobj space,from
R3100 T5573 T5556 punct .,generated
R3101 T5638 T5637 amod intrastrial,space
R3102 T5639 T5621 prep into,transport
R3103 T5575 T5576 det The,potential
R3104 T5640 T5639 pobj endolymph,into
R3105 T5641 T5621 cc and,transport
R3106 T5642 T5621 conj keep,transport
R3107 T5643 T5644 det the,concentration
R3108 T5576 T5577 nsubjpass potential,generated
R3109 T5644 T5642 dobj concentration,keep
R3110 T5645 T5644 nmod K,concentration
R3111 T5646 T5645 punct +,K
R3112 T5578 T5577 auxpass is,generated
R3113 T5647 T5642 oprd low,keep
R3114 T5579 T5577 prep across,generated
R3115 T5648 T5642 advcl adjacent,keep
R3116 T5649 T5648 prep to,adjacent
R3117 T5580 T5581 det the,barrier
R3118 T5650 T5651 det the,channel
R3119 T5651 T5649 pobj channel,to
R3120 T5652 T5651 nmod KCNJ10,channel
R3121 T5581 T5579 pobj barrier,across
R3122 T5653 T5651 nmod K,channel
R3123 T5654 T5653 punct +,K
R3124 T5655 T5656 punct [,26
R3125 T5582 T5583 amod basal,cell
R3126 T5656 T5642 parataxis 26,keep
R3127 T5657 T5656 punct ],26
R3128 T5658 T5621 punct .,transport
R3129 T5660 T5661 aux To,determine
R3130 T5583 T5581 compound cell,barrier
R3131 T5661 T5662 advcl determine,determined
R3132 T5584 T5581 prep of,barrier
R3133 T5663 T5664 det the,cause
R3134 T5664 T5661 dobj cause,determine
R3135 T5665 T5664 prep of,cause
R3136 T5585 T5586 compound stria,vascularis
R3137 T5666 T5667 det the,loss
R3138 T5667 T5665 pobj loss,of
R3139 T5586 T5584 pobj vascularis,of
R3140 T5668 T5667 prep of,loss
R3141 T5669 T5670 det the,potential
R3142 T5670 T5668 pobj potential,of
R3143 T5587 T5577 agent by,generated
R3144 T5671 T5670 amod endocochlear,potential
R3145 T5672 T5667 prep in,loss
R3146 T5673 T5674 nmod Slc26a4,mice
R3147 T5588 T5589 det the,KCNJ10
R3148 T5674 T5672 pobj mice,in
R3149 T5675 T5673 punct -,Slc26a4
R3150 T5676 T5673 punct /,Slc26a4
R3151 T5589 T5587 pobj KCNJ10,by
R3152 T5677 T5673 punct -,Slc26a4
R3153 T5678 T5662 punct ", ",determined
R3154 T5590 T5589 nmod K,KCNJ10
R3155 T5679 T5662 nsubj we,determined
R3156 T5591 T5590 punct +,K
R3157 T5680 T5662 advmod first,determined
R3158 T5592 T5589 compound channel,KCNJ10
R3159 T5681 T5682 mark whether,are
R3160 T5682 T5662 ccomp are,determined
R3161 T5683 T5684 amod intermediate,cells
R3162 T5593 T5589 acl located,KCNJ10
R3163 T5684 T5682 nsubj cells,are
R3164 T5685 T5682 acomp present,are
R3165 T5594 T5593 prep in,located
R3166 T5686 T5682 prep in,are
R3167 T5687 T5688 compound stria,vascularis
R3168 T5688 T5686 pobj vascularis,in
R3169 T5595 T5596 amod intermediate,cells
R3170 T5689 T5662 punct ", ",determined
R3171 T5690 T5691 mark since,known
R3172 T5691 T5662 advcl known,determined
R3173 T5596 T5594 pobj cells,in
R3174 T5692 T5693 det a,loss
R3175 T5693 T5691 nsubjpass loss,known
R3176 T5694 T5693 prep of,loss
R3177 T5597 T5598 punct [,24
R3178 T5695 T5696 amod intermediate,cells
R3179 T5696 T5694 pobj cells,of
R3180 T5697 T5691 auxpass is,known
R3181 T5698 T5699 aux to,lead
R3182 T5598 T5596 parataxis 24,cells
R3183 T5699 T5691 xcomp lead,known
R3184 T5700 T5699 prep to,lead
R3185 T5599 T5598 punct ],24
R3186 T5701 T5702 det a,loss
R3187 T5702 T5700 pobj loss,to
R3188 T5600 T5596 punct ", ",cells
R3189 T5703 T5702 prep of,loss
R3190 T5704 T5705 det the,potential
R3191 T5601 T5602 dep which,connected
R3192 T5705 T5703 pobj potential,of
R3193 T5602 T5596 relcl connected,cells
R3194 T5706 T5705 amod endocochlear,potential
R3195 T5707 T5708 punct [,28
R3196 T5603 T5602 auxpass are,connected
R3197 T5708 T5691 parataxis 28,known
R3198 T5709 T5708 nummod 27,28
R3199 T5710 T5708 punct ",",28
R3200 T5604 T5602 prep to,connected
R3201 T5711 T5708 punct ],28
R3202 T5712 T5662 punct .,determined
R3203 T5605 T5606 amod basal,cells
R3204 T5714 T5715 amod Intermediate,cells
R3205 T5715 T5716 nsubjpass cells,visualized
R3206 T5606 T5604 pobj cells,to
R3207 T5717 T5715 prep of,cells
R3208 T5718 T5719 compound stria,vascularis
R3209 T5607 T5602 prep by,connected
R3210 T5719 T5717 pobj vascularis,of
R3211 T5720 T5716 auxpass were,visualized
R3212 T5608 T5609 det a,density
R3213 T5721 T5716 prep by,visualized
R3214 T5722 T5723 poss their,pigmentation
R3215 T5723 T5721 pobj pigmentation,by
R3216 T5609 T5607 pobj density,by
R3217 T5724 T5716 punct .,visualized
R3218 T5610 T5609 amod high,density
R3219 T5611 T5609 prep of,density
R3220 T5726 T5727 nsubj Pigmentation,was
R3221 T5612 T5613 compound gap,junctions
R3222 T5728 T5727 acomp present,was
R3223 T5729 T5727 prep in,was
R3224 T5613 T5611 pobj junctions,of
R3225 T5730 T5731 compound stria,vascularis
R3226 T5731 T5729 pobj vascularis,in
R3227 T5732 T5731 prep of,vascularis
R3228 T5614 T5615 punct [,25
R3229 T5733 T5734 nmod Slc26a4,mice
R3230 T5615 T5602 parataxis 25,connected
R3231 T5734 T5732 pobj mice,of
R3232 T5735 T5733 punct -,Slc26a4
R3233 T5736 T5733 punct /,Slc26a4
R3234 T5737 T5733 punct -,Slc26a4
R3235 T5738 T5739 punct (,Fig.
R3236 T5616 T5615 punct ],25
R3237 T5739 T5727 parataxis Fig.,was
R3238 T5740 T5739 nummod 2b,Fig.
R3239 T5741 T5739 punct ),Fig.
R3240 T5617 T5577 punct .,generated
R3241 T5742 T5727 punct ", ",was
R3242 T5743 T5744 dep which,suggests
R3243 T5831 T5820 punct /,Slc26a4
R3244 T5744 T5727 advcl suggests,was
R3245 T5745 T5746 mark that,are
R3246 T5746 T5744 ccomp are,suggests
R3247 T5747 T5748 amod intermediate,cells
R3248 T5748 T5746 nsubj cells,are
R3249 T5749 T5746 acomp present,are
R3250 T5750 T5727 punct .,was
R3251 T5752 T5753 advmod Interestingly,was
R3252 T5754 T5753 punct ", ",was
R3253 T5755 T5753 nsubj pigmentation,was
R3254 T5832 T5820 punct -,Slc26a4
R3255 T5756 T5755 prep of,pigmentation
R3256 T5757 T5758 compound stria,vascularis
R3257 T5758 T5756 pobj vascularis,of
R3258 T5833 T5777 punct ", ",isolated
R3259 T5759 T5760 advmod much,stronger
R3260 T5760 T5753 acomp stronger,was
R3261 T5761 T5753 prep in,was
R3262 T5834 T5777 conj assessed,isolated
R3263 T5762 T5761 pobj Slc26a4,in
R3264 T5763 T5762 punct -,Slc26a4
R3265 T5835 T5834 dobj amounts,assessed
R3266 T5764 T5762 punct /,Slc26a4
R3267 T5765 T5762 punct -,Slc26a4
R3268 T5766 T5753 prep than,was
R3269 T5767 T5766 prep in,than
R3270 T5836 T5835 prep of,amounts
R3271 T5768 T5769 nmod Slc26a4,mice
R3272 T5769 T5767 pobj mice,in
R3273 T5770 T5768 punct +,Slc26a4
R3274 T5837 T5838 amod isolated,tissues
R3275 T5771 T5768 punct /,Slc26a4
R3276 T5772 T5768 punct +,Slc26a4
R3277 T5773 T5753 punct .,was
R3278 T5838 T5836 pobj tissues,of
R3279 T5775 T5776 aux To,determine
R3280 T5776 T5777 advcl determine,isolated
R3281 T5839 T5834 prep by,assessed
R3282 T5778 T5776 prep in,determine
R3283 T5779 T5780 amod greater,detail
R3284 T5780 T5778 pobj detail,in
R3285 T5781 T5782 det the,cause
R3286 T5782 T5776 dobj cause,determine
R3287 T5840 T5839 pobj quantification,by
R3288 T5783 T5782 prep of,cause
R3289 T5784 T5785 det the,loss
R3290 T5785 T5783 pobj loss,of
R3291 T5841 T5840 prep of,quantification
R3292 T5786 T5785 prep of,loss
R3293 T5787 T5788 det the,potential
R3294 T5788 T5786 pobj potential,of
R3295 T5842 T5843 compound 18S,rRNA
R3296 T5789 T5788 amod endocochlear,potential
R3297 T5790 T5782 prep in,cause
R3298 T5791 T5792 nmod Slc26a4,mice
R3299 T5843 T5841 pobj rRNA,of
R3300 T5792 T5790 pobj mice,in
R3301 T5793 T5791 punct -,Slc26a4
R3302 T5794 T5791 punct /,Slc26a4
R3303 T5844 T5834 punct ", ",assessed
R3304 T5795 T5791 punct -,Slc26a4
R3305 T5796 T5777 punct ", ",isolated
R3306 T5845 T5834 cc and,assessed
R3307 T5797 T5777 nsubj we,isolated
R3308 T5798 T5799 amod total,RNA
R3309 T5799 T5777 dobj RNA,isolated
R3310 T5846 T5834 conj quantified,assessed
R3311 T5800 T5777 prep from,isolated
R3312 T5801 T5802 compound stria,vascularis
R3313 T5802 T5800 pobj vascularis,from
R3314 T5847 T5848 det the,expression
R3315 T5803 T5802 cc and,vascularis
R3316 T5804 T5805 amod spiral,ganglia
R3317 T5848 T5846 dobj expression,quantified
R3318 T5805 T5802 conj ganglia,vascularis
R3319 T5806 T5802 prep of,vascularis
R332 T770 T771 compound Pendred,syndrome
R3320 T5807 T5808 amod young,Slc26a4
R3321 T5849 T5848 prep of,expression
R3322 T5808 T5806 pobj Slc26a4,of
R3323 T5809 T5810 punct (,month
R3324 T5810 T5808 parataxis month,Slc26a4
R3325 T5850 T5851 compound KCNJ10,mRNA
R3326 T5811 T5812 quantmod 1,4
R3327 T5812 T5810 nummod 4,month
R3328 T5813 T5812 punct –,4
R3329 T5851 T5849 pobj mRNA,of
R333 T771 T772 nsubj syndrome,is
R3330 T5814 T5810 punct ),month
R3331 T5815 T5808 punct +,Slc26a4
R3332 T5816 T5808 punct /,Slc26a4
R3333 T5852 T5777 punct .,isolated
R3334 T5817 T5808 punct +,Slc26a4
R3335 T5818 T5808 cc and,Slc26a4
R3336 T5819 T5820 preconj both,Slc26a4
R3337 T5854 T5855 nsubj Quantities,were
R3338 T5820 T5829 nmod Slc26a4,mice
R3339 T5821 T5820 amod young,Slc26a4
R334 T773 T774 det a,disorder
R3340 T5822 T5820 cc and,Slc26a4
R3341 T5823 T5820 amod old,Slc26a4
R3342 T5856 T5854 prep of,Quantities
R3343 T5824 T5825 punct (,month
R3344 T5825 T5820 parataxis month,Slc26a4
R3345 T5826 T5827 punct ~,12
R3346 T5827 T5825 nummod 12,month
R3347 T5857 T5858 compound stria,vascularis
R3348 T5828 T5825 punct ),month
R3349 T5829 T5808 conj mice,Slc26a4
R335 T774 T772 attr disorder,is
R3350 T5830 T5820 punct -,Slc26a4
R3351 T5858 T5856 pobj vascularis,of
R3352 T5859 T5854 acl isolated,Quantities
R3353 T5860 T5859 prep from,isolated
R3354 T5937 T5917 punct ),0.18
R3355 T5861 T5862 det these,mice
R3356 T5938 T5939 advmod significantly,smaller
R3357 T5862 T5860 pobj mice,from
R3358 T5939 T5899 acomp smaller,were
R3359 T5940 T5939 prep than,smaller
R336 T775 T776 advmod relatively,common
R3360 T5941 T5940 prep in,than
R3361 T5863 T5862 amod different,mice
R3362 T5942 T5943 nmod Slc26a4,mice
R3363 T5943 T5941 pobj mice,in
R3364 T5864 T5855 acomp similar,were
R3365 T5944 T5942 punct +,Slc26a4
R3366 T5945 T5942 punct /,Slc26a4
R3367 T5865 T5855 punct ", ",were
R3368 T5946 T5942 punct +,Slc26a4
R3369 T5947 T5948 punct (,7
R337 T776 T774 amod common,disorder
R3370 T5866 T5867 mark since,found
R3371 T5948 T5943 parataxis 7,mice
R3372 T5949 T5950 nmod log,rRNA
R3373 T5950 T5952 nsubj rRNA,0.19
R3374 T5867 T5855 advcl found,were
R3375 T5951 T5950 punct (,rRNA
R3376 T5952 T5948 ccomp 0.19,7
R3377 T5868 T5869 det no,differences
R3378 T5953 T5950 punct ),rRNA
R3379 T5954 T5952 punct =,0.19
R338 T777 T778 amod autosomal,recessive
R3380 T5955 T5952 nummod 9.48,0.19
R3381 T5869 T5867 nsubjpass differences,found
R3382 T5956 T5952 punct ±,0.19
R3383 T5957 T5948 punct ", ",7
R3384 T5958 T5948 nsubj n,7
R3385 T5870 T5869 amod significant,differences
R3386 T5959 T5948 punct =,7
R3387 T5960 T5948 punct ),7
R3388 T5961 T5899 punct ", ",were
R3389 T5962 T5899 advcl consistent,were
R339 T778 T774 amod recessive,disorder
R3390 T5963 T5962 prep with,consistent
R3391 T5964 T5965 amod morphometric,data
R3392 T5871 T5867 auxpass were,found
R3393 T5965 T5963 pobj data,with
R3394 T5966 T5967 punct (,see
R3395 T5967 T5899 parataxis see,were
R3396 T5872 T5867 prep in,found
R3397 T5968 T5967 advmod below,see
R3398 T5969 T5967 punct ),see
R3399 T5970 T5899 punct .,were
R340 T779 T778 punct -,recessive
R3400 T5873 T5874 det the,numbers
R3401 T5972 T5973 nsubj Expression,was
R3402 T5874 T5872 pobj numbers,in
R3403 T5974 T5972 prep of,Expression
R3404 T5975 T5976 compound KCNJ10,mRNA
R3405 T5976 T5974 pobj mRNA,of
R3406 T5875 T5874 prep of,numbers
R3407 T5977 T5973 acomp normal,was
R3408 T5978 T5977 prep in,normal
R3409 T5979 T5980 compound stria,vascularis
R341 T780 T774 acl characterized,disorder
R3410 T5876 T5877 compound 18S,molecules
R3411 T5980 T5978 pobj vascularis,in
R3412 T5981 T5980 cc and,vascularis
R3413 T5982 T5983 amod spiral,ganglia
R3414 T5877 T5875 pobj molecules,of
R3415 T5983 T5980 conj ganglia,vascularis
R3416 T5984 T5980 prep of,vascularis
R3417 T5985 T5986 amod young,mice
R3418 T5878 T5877 compound rRNA,molecules
R3419 T5879 T5880 punct (,data
R342 T781 T780 agent by,characterized
R3420 T5986 T5984 pobj mice,of
R3421 T5987 T5986 nmod Slc26a4,mice
R3422 T5880 T5867 parataxis data,found
R3423 T5988 T5987 punct -,Slc26a4
R3424 T5989 T5987 punct /,Slc26a4
R3425 T5881 T5882 nmod log,rRNA
R3426 T5990 T5987 punct -,Slc26a4
R3427 T5991 T5977 cc but,normal
R3428 T5992 T5993 advmod significantly,reduced
R3429 T5993 T5977 conj reduced,normal
R343 T782 T781 pobj deafness,by
R3430 T5882 T5884 nsubj rRNA,0.08
R3431 T5994 T5993 prep in,reduced
R3432 T5995 T5996 amod old,mice
R3433 T5883 T5882 punct (,rRNA
R3434 T5996 T5994 pobj mice,in
R3435 T5997 T5996 nmod Slc26a4,mice
R3436 T5998 T5997 punct -,Slc26a4
R3437 T5999 T5997 punct /,Slc26a4
R3438 T5884 T5880 ccomp 0.08,data
R3439 T6000 T5997 punct -,Slc26a4
R344 T783 T782 cc and,deafness
R3440 T6001 T6002 punct (,Fig.
R3441 T6002 T5993 parataxis Fig.,reduced
R3442 T6003 T6002 nummod 3,Fig.
R3443 T6004 T6002 punct ),Fig.
R3444 T6005 T5973 punct .,was
R3445 T5885 T5882 punct ),rRNA
R3446 T6007 T6008 nsubjpass Quantifications,used
R3447 T5886 T5884 punct =,0.08
R3448 T6009 T6007 prep of,Quantifications
R3449 T6010 T6011 nmod KCNQ1,mRNA
R345 T784 T782 conj goiter,deafness
R3450 T6011 T6009 pobj mRNA,of
R3451 T5887 T5884 nummod 9.46,0.08
R3452 T6012 T6010 cc and,KCNQ1
R3453 T6013 T6010 conj KCNQ4,KCNQ1
R3454 T6014 T6008 auxpass were,used
R3455 T5888 T5884 punct ±,0.08
R3456 T6015 T6016 aux to,assess
R3457 T6016 T6008 advcl assess,used
R3458 T5889 T5880 punct ", ",data
R3459 T6017 T6018 amod possible,cross-contamination
R346 T785 T786 punct [,1
R3460 T6018 T6016 dobj cross-contamination,assess
R3461 T6019 T6018 prep between,cross-contamination
R3462 T5890 T5891 nsubj n,17
R3463 T6020 T6021 det the,preparations
R3464 T6021 T6019 pobj preparations,between
R3465 T6022 T6023 nmod stria,vascularis
R3466 T5891 T5880 ccomp 17,data
R3467 T6023 T6021 nmod vascularis,preparations
R3468 T6024 T6023 cc and,vascularis
R3469 T6025 T6026 amod spiral,ganglia
R347 T786 T780 parataxis 1,characterized
R3470 T5892 T5891 punct =,17
R3471 T6026 T6023 conj ganglia,vascularis
R3472 T6027 T6008 prep based,used
R3473 T6028 T6027 prep on,based
R3474 T5893 T5880 punct ", ",data
R3475 T6029 T6030 det the,assumptions
R3476 T6030 T6028 pobj assumptions,on
R3477 T5894 T5880 amod pooled,data
R3478 T6031 T6032 mark that,expressed
R3479 T6032 T6030 acl expressed,assumptions
R348 T787 T786 punct ],1
R3480 T6033 T6034 punct (,1
R3481 T5895 T5880 punct ),data
R3482 T6034 T6032 meta 1,expressed
R3483 T6035 T6034 punct ),1
R3484 T6036 T6032 nsubjpass KCNQ1,expressed
R3485 T6037 T6032 auxpass is,expressed
R3486 T5896 T5855 punct .,were
R3487 T6038 T6032 prep in,expressed
R3488 T5898 T5899 prep In,were
R3489 T6039 T6038 pobj cells,in
R349 T788 T772 punct .,is
R3490 T6040 T6039 prep of,cells
R3491 T6041 T6042 det the,vascularis
R3492 T5900 T5898 pobj contrast,In
R3493 T5901 T5899 punct ", ",were
R3494 T6042 T6040 pobj vascularis,of
R3495 T6043 T6042 compound stria,vascularis
R3496 T5902 T5899 nsubj quantities,were
R3497 T6044 T6042 cc but,vascularis
R3498 T6045 T6044 neg not,but
R3499 T5903 T5902 prep of,quantities
R350 T790 T791 det The,syndrome
R3500 T6046 T6047 det the,preparation
R3501 T6047 T6042 conj preparation,vascularis
R3502 T6048 T6047 amod spiral,preparation
R3503 T5904 T5905 amod spiral,ganglia
R3504 T6049 T6047 compound ganglia,preparation
R3505 T6050 T6032 cc and,expressed
R3506 T6051 T6052 punct (,2
R3507 T5905 T5903 pobj ganglia,of
R3508 T6052 T6053 meta 2,expressed
R3509 T6053 T6032 conj expressed,expressed
R351 T791 T792 nsubjpass syndrome,caused
R3510 T6054 T6052 punct ),2
R3511 T6055 T6053 nsubjpass KCNQ4,expressed
R3512 T5906 T5902 acl isolated,quantities
R3513 T6056 T6053 auxpass is,expressed
R3514 T6057 T6053 prep in,expressed
R3515 T5907 T5906 prep from,isolated
R3516 T6058 T6057 pobj cells,in
R3517 T6059 T6058 prep of,cells
R3518 T6060 T6061 det the,ganglia
R3519 T5908 T5909 amod young,mice
R352 T793 T792 auxpass is,caused
R3520 T6061 T6059 pobj ganglia,of
R3521 T5909 T5907 pobj mice,from
R3522 T5910 T5908 cc and,young
R3523 T6062 T6061 amod spiral,ganglia
R3524 T5911 T5908 conj old,young
R3525 T6063 T6061 cc but,ganglia
R3526 T6064 T6063 neg not,but
R3527 T6065 T6066 det the,preparation
R3528 T5912 T5909 nmod Slc26a4,mice
R3529 T6066 T6061 conj preparation,ganglia
R353 T794 T792 agent by,caused
R3530 T6067 T6066 compound stria,preparation
R3531 T6068 T6066 compound vascularis,preparation
R3532 T5913 T5912 punct -,Slc26a4
R3533 T6069 T6008 punct .,used
R3534 T6071 T6072 det The,number
R3535 T5914 T5912 punct /,Slc26a4
R3536 T5915 T5912 punct -,Slc26a4
R3537 T6072 T6073 nsubj number,was
R3538 T5916 T5917 punct (,0.18
R3539 T6074 T6072 prep of,number
R354 T795 T794 pobj mutations,by
R3540 T5917 T5902 parataxis 0.18,quantities
R3541 T6075 T6076 compound KCNQ1,molecules
R3542 T6076 T6074 pobj molecules,of
R3543 T6077 T6076 compound mRNA,molecules
R3544 T5918 T5919 nmod log,rRNA
R3545 T6078 T6072 prep in,number
R3546 T6079 T6080 compound stria,vascularis
R3547 T6080 T6078 pobj vascularis,in
R3548 T6081 T6082 npadvmod 24,greater
R3549 T6082 T6073 acomp greater,was
R355 T796 T795 prep of,mutations
R3550 T5919 T5917 nsubj rRNA,0.18
R3551 T6083 T6081 punct -,24
R3552 T6084 T6081 advmod fold,24
R3553 T6085 T6082 prep than,greater
R3554 T5920 T5919 punct (,rRNA
R3555 T6086 T6085 prep in,than
R3556 T6087 T6088 amod spiral,ganglia
R3557 T6088 T6086 pobj ganglia,in
R3558 T5921 T5919 punct ),rRNA
R3559 T6089 T6073 punct ", ",was
R356 T797 T798 det the,gene
R3560 T6090 T6073 cc and,was
R3561 T5922 T5917 punct =,0.18
R3562 T6091 T6092 det the,number
R3563 T6092 T6093 nsubj number,was
R3564 T6093 T6073 conj was,was
R3565 T5923 T5917 quantmod 9.04,0.18
R3566 T6094 T6092 prep of,number
R3567 T6095 T6096 compound KCNQ4,molecules
R3568 T6096 T6094 pobj molecules,of
R3569 T5924 T5917 punct ±,0.18
R357 T798 T796 pobj gene,of
R3570 T6097 T6096 compound mRNA,molecules
R3571 T6098 T6092 prep in,number
R3572 T6099 T6100 amod spiral,ganglia
R3573 T5925 T5926 punct ", ",4
R3574 T6100 T6098 pobj ganglia,in
R3575 T6101 T6102 npadvmod 5,greater
R3576 T6102 T6093 acomp greater,was
R3577 T5926 T5917 parataxis 4,0.18
R3578 T6103 T6101 punct -,5
R3579 T6104 T6101 advmod fold,5
R358 T799 T798 compound PDS,gene
R3580 T6105 T6102 prep than,greater
R3581 T5927 T5926 nsubj n,4
R3582 T6106 T6105 prep in,than
R3583 T6107 T6108 compound stria,vascularis
R3584 T6108 T6106 pobj vascularis,in
R3585 T5928 T5926 punct =,4
R3586 T6109 T6093 punct .,was
R3587 T6111 T6112 det These,observations
R3588 T5929 T5917 cc and,0.18
R3589 T6112 T6113 nsubj observations,validate
R359 T800 T798 appos SLC26A4,gene
R3590 T5930 T5931 quantmod 9.29,0.15
R3591 T6114 T6115 poss our,measurements
R3592 T6115 T6113 dobj measurements,validate
R3593 T6116 T6115 prep of,measurements
R3594 T5931 T5917 conj 0.15,0.18
R3595 T6117 T6116 pobj KCNJ10,of
R3596 T6118 T6117 cc and,KCNJ10
R3597 T6119 T6117 conj KCNQ1,KCNJ10
R3598 T6120 T6113 prep by,validate
R3599 T6121 T6120 pcomp demonstrating,by
R360 T801 T798 punct ", ",gene
R3600 T6122 T6123 mark that,were
R3601 T5932 T5931 punct ±,0.15
R3602 T6123 T6121 ccomp were,demonstrating
R3603 T6124 T6125 det the,preparations
R3604 T6125 T6123 nsubj preparations,were
R3605 T5933 T5934 punct ", ",6
R3606 T6126 T6125 amod microdissected,preparations
R3607 T6127 T6125 prep of,preparations
R3608 T6128 T6129 compound stria,vascularis
R3609 T5934 T5931 parataxis 6,0.15
R361 T802 T803 dep which,codes
R3610 T6129 T6127 pobj vascularis,of
R3611 T6130 T6129 cc and,vascularis
R3612 T6131 T6132 amod spiral,ganglia
R3613 T5935 T5934 nsubj n,6
R3614 T6132 T6129 conj ganglia,vascularis
R3615 T6133 T6134 nummod 98,%
R3616 T6134 T6135 npadvmod %,pure
R3617 T5936 T5934 punct =,6
R3618 T6135 T6123 acomp pure,were
R3619 T6136 T6134 cc and,%
R362 T803 T798 relcl codes,gene
R3620 T6137 T6138 nummod 78,%
R3621 T6147 T6148 amod r,es
R3622 T6138 T6134 conj %,%
R3623 T6139 T6123 punct ", ",were
R3624 T6140 T6123 advmod respectively,were
R3625 T6141 T6113 punct .,validate
R3626 T6149 T6148 acl en,es
R3627 T6151 T6152 nmod c,e
R3628 T6157 T6156 punct ,KCNJ10
R3629 T6154 T6153 prep f,o
R363 T804 T803 prep for,codes
R3630 T6164 T6165 compound tria,vascularis
R3631 T6162 T6163 nsubj s,supports
R3632 T6166 T6165 prep of,vascularis
R3633 T6167 T6168 nmod Slc26a4,mice
R3634 T6168 T6166 pobj mice,of
R3635 T6250 T6251 amod Histological,evaluation
R3636 T6251 T6252 nsubj evaluation,revealed
R3637 T6169 T6167 punct -,Slc26a4
R3638 T6253 T6251 prep of,evaluation
R3639 T6254 T6253 pobj cryosections,of
R364 T805 T806 det the,pendrin
R3640 T6170 T6167 punct /,Slc26a4
R3641 T6255 T6256 det an,enlargement
R3642 T6256 T6252 dobj enlargement,revealed
R3643 T6171 T6167 punct -,Slc26a4
R3644 T6257 T6256 prep of,enlargement
R3645 T6258 T6259 compound scala,media
R3646 T6259 T6257 pobj media,of
R3647 T6172 T6173 det the,finding
R3648 T6260 T6256 prep with,enlargement
R3649 T6261 T6262 det a,bulging
R365 T806 T804 pobj pendrin,for
R3650 T6262 T6260 pobj bulging,with
R3651 T6263 T6262 amod large,bulging
R3652 T6264 T6262 prep of,bulging
R3653 T6173 T6163 dobj finding,supports
R3654 T6265 T6266 poss Reissner,membrane
R3655 T6266 T6264 pobj membrane,of
R3656 T6267 T6265 case 's,Reissner
R3657 T6268 T6262 cc and,bulging
R3658 T6174 T6175 mark that,are
R3659 T6269 T6270 det an,degeneration
R366 T807 T806 compound protein,pendrin
R3660 T6270 T6262 conj degeneration,bulging
R3661 T6271 T6270 amod apparent,degeneration
R3662 T6175 T6173 acl are,finding
R3663 T6272 T6270 prep of,degeneration
R3664 T6273 T6274 det the,organ
R3665 T6274 T6272 pobj organ,of
R3666 T6176 T6177 amod intermediate,cells
R3667 T6275 T6274 prep of,organ
R3668 T6276 T6275 pobj Corti,of
R3669 T6177 T6175 nsubj cells,are
R367 T808 T809 punct [,2
R3670 T6178 T6175 acomp present,are
R3671 T6277 T6252 punct ", ",revealed
R3672 T6179 T6163 punct .,supports
R3673 T6278 T6279 mark as,described
R3674 T6279 T6252 advcl described,revealed
R3675 T6280 T6279 advmod earlier,described
R3676 T6181 T6182 nsubjpass Expression,assessed
R3677 T6281 T6282 punct [,14
R3678 T6282 T6252 parataxis 14,revealed
R3679 T6283 T6282 punct ],14
R368 T809 T803 parataxis 2,codes
R3680 T6183 T6181 prep of,Expression
R3681 T6284 T6252 punct .,revealed
R3682 T6286 T6287 compound Tissue,height
R3683 T6287 T6288 nsubj height,was
R3684 T6184 T6185 det the,protein
R3685 T6289 T6287 prep of,height
R3686 T6185 T6183 pobj protein,of
R3687 T6290 T6291 compound stria,vascularis
R3688 T6291 T6289 pobj vascularis,of
R3689 T6292 T6288 acomp normal,was
R369 T810 T809 punct ],2
R3690 T6186 T6185 compound KCNJ10,protein
R3691 T6293 T6294 punct (,Fig.
R3692 T6294 T6288 parataxis Fig.,was
R3693 T6187 T6182 auxpass was,assessed
R3694 T6295 T6294 nummod 5,Fig.
R3695 T6296 T6294 punct ),Fig.
R3696 T6297 T6288 punct ", ",was
R3697 T6188 T6182 prep in,assessed
R3698 T6298 T6288 advcl consistent,was
R3699 T6299 T6298 prep with,consistent
R370 T811 T792 punct .,caused
R3700 T6300 T6301 det the,numbers
R3701 T6301 T6299 pobj numbers,with
R3702 T6302 T6301 amod similar,numbers
R3703 T6303 T6301 prep of,numbers
R3704 T6189 T6190 amod young,mice
R3705 T6304 T6305 compound 18S,molecules
R3706 T6305 T6303 pobj molecules,of
R3707 T6306 T6305 compound rRNA,molecules
R3708 T6190 T6188 pobj mice,in
R3709 T6307 T6301 prep in,numbers
R371 T813 T814 nsubj Deafness,is
R3710 T6308 T6309 amod isolated,preparations
R3711 T6309 T6307 pobj preparations,in
R3712 T6191 T6190 nmod Slc26a4,mice
R3713 T6310 T6311 punct (,see
R3714 T6311 T6298 parataxis see,consistent
R3715 T6312 T6311 advmod above,see
R3716 T6192 T6191 punct -,Slc26a4
R3717 T6313 T6311 punct ),see
R3718 T6314 T6288 punct .,was
R3719 T6193 T6191 punct /,Slc26a4
R372 T815 T814 acomp congenital,is
R3720 T6316 T6317 det The,absence
R3721 T6317 T6318 nsubj absence,is
R3722 T6194 T6191 punct -,Slc26a4
R3723 T6319 T6317 prep of,absence
R3724 T6320 T6321 det a,change
R3725 T6321 T6319 pobj change,of
R3726 T6195 T6182 prep by,assessed
R3727 T6322 T6321 prep in,change
R3728 T6323 T6324 compound tissue,height
R3729 T6324 T6322 pobj height,in
R373 T816 T815 cc and,congenital
R3730 T6196 T6197 amod confocal,immunocytochemistry
R3731 T6325 T6318 acomp consistent,is
R3732 T6326 T6325 prep with,consistent
R3733 T6197 T6195 pobj immunocytochemistry,by
R3734 T6327 T6328 det the,presence
R3735 T6328 T6326 pobj presence,with
R3736 T6329 T6328 prep of,presence
R3737 T6198 T6182 punct .,assessed
R3738 T6330 T6331 amod intermediate,cells
R3739 T6331 T6329 pobj cells,of
R374 T817 T818 advmod generally,profound
R3740 T6332 T6333 punct [,28
R3741 T6200 T6201 advmod Interestingly,was
R3742 T6333 T6318 parataxis 28,is
R3743 T6334 T6333 punct ],28
R3744 T6335 T6318 punct .,is
R3745 T6202 T6201 punct ", ",was
R3746 T6337 T6338 advmod Further,observed
R3747 T6339 T6338 punct ", ",observed
R3748 T6340 T6338 nsubj we,observed
R3749 T6203 T6201 nsubj expression,was
R375 T818 T815 conj profound,congenital
R3750 T6341 T6342 det an,loss
R3751 T6342 T6338 dobj loss,observed
R3752 T6343 T6342 amod apparent,loss
R3753 T6204 T6203 prep of,expression
R3754 T6344 T6342 prep of,loss
R3755 T6345 T6346 compound tissue,masses
R3756 T6205 T6206 det the,protein
R3757 T6346 T6344 pobj masses,of
R3758 T6206 T6204 pobj protein,of
R3759 T6347 T6338 prep in,observed
R376 T819 T820 mark although,late
R3760 T6348 T6347 pobj areas,in
R3761 T6349 T6350 dep that,occupied
R3762 T6207 T6206 compound KCNJ10,protein
R3763 T6350 T6348 relcl occupied,areas
R3764 T6351 T6350 auxpass are,occupied
R3765 T6352 T6350 advmod normally,occupied
R3766 T6208 T6201 acomp absent,was
R3767 T6353 T6354 advmod primarily,by
R3768 T6354 T6350 agent by,occupied
R3769 T6355 T6356 nmod type,I
R377 T820 T818 advcl late,profound
R3770 T6209 T6201 prep in,was
R3771 T6356 T6357 nummod I,fibrocytes
R3772 T6357 T6354 pobj fibrocytes,by
R3773 T6210 T6211 compound stria,vascularis
R3774 T6358 T6356 cc and,I
R3775 T6359 T6356 conj II,I
R3776 T6360 T6338 punct .,observed
R3777 T6211 T6209 pobj vascularis,in
R3778 T6362 T6363 amod Spiral,prominence
R3779 T6212 T6201 cc but,was
R378 T821 T820 advmod sometimes,late
R3780 T6363 T6364 nsubj prominence,was
R3781 T6365 T6363 prep in,prominence
R3782 T6213 T6201 conj normal,was
R3783 T6366 T6367 nmod Slc26a4,mice
R3784 T6214 T6213 prep in,normal
R3785 T6367 T6365 pobj mice,in
R3786 T6368 T6366 punct -,Slc26a4
R3787 T6215 T6216 amod spiral,ganglia
R3788 T6369 T6366 punct /,Slc26a4
R3789 T6370 T6366 punct -,Slc26a4
R379 T822 T820 prep in,late
R3790 T6371 T6372 advmod less,prominent
R3791 T6216 T6214 pobj ganglia,in
R3792 T6372 T6364 acomp prominent,was
R3793 T6373 T6364 punct ", ",was
R3794 T6374 T6375 amod spiral,ligament
R3795 T6217 T6218 punct (,Fig.
R3796 T6375 T6376 nsubj ligament,thinner
R3797 T6376 T6364 conj thinner,was
R3798 T6218 T6213 parataxis Fig.,normal
R3799 T6377 T6376 cc and,thinner
R380 T823 T822 pobj onset,in
R3800 T6378 T6379 amod spiral,limbus
R3801 T6379 T6380 nsubj limbus,flatter
R3802 T6380 T6376 conj flatter,thinner
R3803 T6219 T6218 nummod 4,Fig.
R3804 T6381 T6382 punct (,Fig.
R3805 T6382 T6380 parataxis Fig.,flatter
R3806 T6383 T6382 nummod 5,Fig.
R3807 T6384 T6382 punct ),Fig.
R3808 T6385 T6380 punct .,flatter
R3809 T6220 T6218 punct ),Fig.
R381 T824 T815 cc and,congenital
R3810 T6387 T6388 det The,observation
R3811 T6388 T6389 nsubj observation,is
R3812 T6221 T6201 punct .,was
R3813 T6390 T6391 mark that,lost
R3814 T6391 T6388 acl lost,observation
R3815 T6392 T6393 compound tissue,masses
R3816 T6223 T6224 det The,absence
R3817 T6393 T6391 nsubjpass masses,lost
R3818 T6394 T6391 auxpass were,lost
R3819 T6395 T6391 prep in,lost
R382 T825 T815 conj provoked,congenital
R3820 T6396 T6397 det the,region
R3821 T6224 T6225 nsubj absence,is
R3822 T6397 T6395 pobj region,in
R3823 T6398 T6399 amod spiral,limbus
R3824 T6399 T6397 compound limbus,region
R3825 T6226 T6224 prep of,absence
R3826 T6400 T6389 acomp consistent,is
R3827 T6401 T6400 prep with,consistent
R3828 T6402 T6403 det the,finding
R3829 T6403 T6401 pobj finding,with
R383 T826 T825 agent by,provoked
R3830 T6227 T6228 det the,channel
R3831 T6404 T6403 prep of,finding
R3832 T6405 T6406 det a,number
R3833 T6406 T6404 pobj number,of
R3834 T6228 T6226 pobj channel,of
R3835 T6407 T6406 amod reduced,number
R3836 T6408 T6406 prep of,number
R3837 T6409 T6410 compound 18S,rRNA
R3838 T6229 T6228 nmod KCNJ10,channel
R3839 T6410 T6411 compound rRNA,molecules
R384 T827 T828 amod light,injury
R3840 T6411 T6408 pobj molecules,of
R3841 T6412 T6406 prep in,number
R3842 T6230 T6228 nmod K,channel
R3843 T6413 T6414 det the,preparation
R3844 T6414 T6412 pobj preparation,in
R3845 T6415 T6416 amod spiral,ganglia
R3846 T6231 T6230 punct +,K
R3847 T6416 T6414 compound ganglia,preparation
R3848 T6417 T6418 punct (,see
R3849 T6418 T6389 parataxis see,is
R385 T828 T826 pobj injury,by
R3850 T6232 T6224 prep in,absence
R3851 T6419 T6418 advmod above,see
R3852 T6420 T6418 punct ),see
R3853 T6421 T6389 punct .,is
R3854 T6423 T6424 prep In,observed
R3855 T6233 T6234 compound stria,vascularis
R3856 T6425 T6423 pobj addition,In
R3857 T6426 T6424 punct ", ",observed
R3858 T6427 T6424 nsubj we,observed
R3859 T6428 T6429 det an,degeneration
R386 T829 T828 compound head,injury
R3860 T6429 T6424 dobj degeneration,observed
R3861 T6234 T6232 pobj vascularis,in
R3862 T6430 T6429 amod apparent,degeneration
R3863 T6431 T6429 prep of,degeneration
R3864 T6432 T6433 compound stria,vascularis
R3865 T6235 T6225 acomp sufficient,is
R3866 T6433 T6431 pobj vascularis,of
R3867 T6434 T6429 punct ", ",degeneration
R3868 T6435 T6429 prep including,degeneration
R3869 T6236 T6237 aux to,explain
R387 T830 T814 punct .,is
R3870 T6436 T6437 det an,increase
R3871 T6437 T6435 pobj increase,including
R3872 T6438 T6437 prep in,increase
R3873 T6237 T6235 xcomp explain,sufficient
R3874 T6439 T6438 pobj pigmentation,in
R3875 T6440 T6437 cc and,increase
R3876 T6441 T6442 det an,pattern
R3877 T6238 T6239 det the,loss
R3878 T6442 T6437 conj pattern,increase
R3879 T6443 T6442 amod irregular,pattern
R388 T832 T833 amod Vestibular,dysfunction
R3880 T6239 T6237 dobj loss,explain
R3881 T6444 T6442 prep of,pattern
R3882 T6445 T6446 det the,junctions
R3883 T6446 T6444 pobj junctions,of
R3884 T6240 T6239 prep of,loss
R3885 T6447 T6446 amod tight,junctions
R3886 T6448 T6446 prep of,junctions
R3887 T6449 T6450 amod marginal,cells
R3888 T6241 T6242 det the,potential
R3889 T6450 T6448 pobj cells,of
R389 T833 T834 nsubj dysfunction,is
R3890 T6451 T6452 punct (,7
R3891 T6242 T6240 pobj potential,of
R3892 T6243 T6242 amod endocochlear,potential
R3893 T6452 T6424 parataxis 7,observed
R3894 T6244 T6245 punct [,17
R3895 T6453 T6452 dep Fig.,7
R3896 T6454 T6453 nummod 2c,Fig.
R3897 T6455 T6452 punct ", ",7
R3898 T6245 T6225 parataxis 17,is
R3899 T6456 T6452 nummod 6,7
R390 T835 T834 acomp uncommon,is
R3900 T6457 T6452 punct ",",7
R3901 T6458 T6452 punct ),7
R3902 T6246 T6245 punct ],17
R3903 T6459 T6424 punct .,observed
R3904 T6247 T6225 punct .,is
R3905 T6461 T6462 amod Tight,junctions
R3906 T6462 T6463 nsubjpass junctions,visualized
R3907 T6496 T6497 nsubjpass s,i
R3908 T6464 T6463 auxpass were,visualized
R3909 T6465 T6463 prep by,visualized
R391 T836 T834 punct .,is
R3910 T6466 T6467 compound F,actin
R3911 T6467 T6469 compound actin,expression
R3912 T6468 T6467 punct -,actin
R3913 T6469 T6465 pobj expression,by
R3914 T6470 T6463 punct .,visualized
R3915 T6472 T6473 amod Marginal,cells
R3916 T6473 T6474 nsubj cells,appeared
R3917 T6475 T6476 aux to,form
R3918 T6476 T6474 xcomp form,appeared
R3919 T6477 T6478 det a,layer
R392 T838 T839 nsubj Goiter,is
R3920 T6478 T6476 dobj layer,form
R3921 T6479 T6478 amod continuous,layer
R3922 T6480 T6474 punct .,appeared
R3923 T6531 T6532 nsubj p,romi
R3924 T6485 T6483 advcl ndr,e
R3925 T6487 T6486 amod i,n
R3926 T6506 T6507 det a,e
R3927 T6535 T6536 amod e,n
R3928 T6507 T6505 pobj e,f
R3929 T6508 T6507 amod c,e
R393 T840 T839 acomp variable,is
R3930 T6509 T6507 compound e,e
R3931 T6546 T6545 auxpass a,re
R3932 T6518 T6519 amod al,s
R3933 T6520 T6519 compound cell,s
R3934 T6521 T6517 prep in,li
R3935 T6547 T6545 advcl l,re
R3936 T6550 T6549 compound c,a
R3937 T6564 T6565 amod e,r
R3938 T6569 T6570 amod basal,cells
R3939 T6570 T6568 nsubj cells,form
R394 T841 T839 cc and,is
R3940 T6571 T6570 punct ", ",cells
R3941 T6572 T6573 dep which,are
R3942 T6573 T6570 relcl are,cells
R3943 T6574 T6573 acomp interconnected,are
R3944 T6575 T6573 prep by,are
R3945 T6565 T6563 pobj r,wh
R3946 T6576 T6577 amod tight,junctions
R3947 T6577 T6575 pobj junctions,by
R3948 T6668 T6664 prep with,junction
R3949 T6578 T6568 punct ", ",form
R395 T842 T843 advmod generally,develops
R3950 T6579 T6580 amod additional,junctions
R3951 T6580 T6568 dobj junctions,form
R3952 T6581 T6580 amod tight,junctions
R3953 T6582 T6580 prep with,junctions
R3954 T6583 T6584 nmod surface,cells
R3955 T6584 T6582 pobj cells,with
R3956 T6585 T6584 amod epithelial,cells
R3957 T6586 T6587 punct [,29
R3958 T6669 T6670 nmod surface,cells
R3959 T6588 T6587 punct ],29
R396 T843 T839 conj develops,is
R3960 T6670 T6668 pobj cells,with
R3961 T6591 T6592 det A,discontinuity
R3962 T6592 T6593 nsubj discontinuity,explain
R3963 T6594 T6592 prep of,discontinuity
R3964 T6595 T6596 det this,junction
R3965 T6671 T6670 amod epithelial,cells
R3966 T6596 T6594 pobj junction,of
R3967 T6597 T6596 amod complex,junction
R3968 T6598 T6592 prep in,discontinuity
R3969 T6672 T6654 prep in,revealed
R397 T844 T843 prep around,develops
R3970 T6599 T6600 nmod Slc26a4,mice
R3971 T6600 T6598 pobj mice,in
R3972 T6601 T6599 punct -,Slc26a4
R3973 T6673 T6674 nmod Slc26a4,mice
R3974 T6602 T6599 punct /,Slc26a4
R3975 T6603 T6599 punct -,Slc26a4
R3976 T6604 T6593 aux would,explain
R3977 T6674 T6672 pobj mice,in
R3978 T6605 T6606 det the,absence
R3979 T6606 T6593 dobj absence,explain
R398 T845 T844 pobj puberty,around
R3980 T6607 T6606 prep of,absence
R3981 T6675 T6673 punct -,Slc26a4
R3982 T6608 T6609 det the,potential
R3983 T6609 T6607 pobj potential,of
R3984 T6610 T6609 amod endocochlear,potential
R3985 T6676 T6673 punct /,Slc26a4
R3986 T6611 T6593 punct .,explain
R3987 T6613 T6614 aux To,evaluate
R3988 T6677 T6673 punct -,Slc26a4
R3989 T6614 T6615 advcl evaluate,determined
R399 T846 T847 punct [,3
R3990 T6616 T6617 det the,presence
R3991 T6678 T6654 punct ", ",revealed
R3992 T6617 T6614 dobj presence,evaluate
R3993 T6618 T6617 prep of,presence
R3994 T6619 T6620 det this,connection
R3995 T6679 T6680 mark as,observed
R3996 T6620 T6618 pobj connection,of
R3997 T6621 T6615 punct ", ",determined
R3998 T6680 T6654 advcl observed,revealed
R3999 T6622 T6615 nsubj we,determined
R400 T847 T843 parataxis 3,develops
R4000 T6623 T6615 prep by,determined
R4001 T6624 T6625 amod confocal,immunocytochemistry
R4002 T6681 T6680 prep in,observed
R4003 T6625 T6623 pobj immunocytochemistry,by
R4004 T6626 T6627 det the,expression
R4005 T6627 T6615 dobj expression,determined
R4006 T6682 T6683 amod normal,mice
R4007 T6628 T6627 prep of,expression
R4008 T6629 T6628 pobj ZO,of
R4009 T6683 T6681 pobj mice,in
R401 T848 T847 punct ],3
R4010 T6630 T6629 punct -,ZO
R4011 T6684 T6685 punct (,7
R4012 T6631 T6629 nummod 1,ZO
R4013 T6632 T6628 cc and,of
R4014 T6633 T6628 conj of,of
R4015 T6634 T6635 compound F,actin
R4016 T6635 T6633 pobj actin,of
R4017 T6636 T6635 punct -,actin
R4018 T6685 T6683 parataxis 7,mice
R4019 T6637 T6633 punct ", ",of
R402 T849 T839 punct .,is
R4020 T6638 T6639 dep which,associate
R4021 T6686 T6687 nmod Fig.,1f
R4022 T6639 T6633 conj associate,of
R4023 T6640 T6639 prep with,associate
R4024 T6641 T6642 amod tight,complexes
R4025 T6687 T6685 dep 1f,7
R4026 T6642 T6640 pobj complexes,with
R4027 T6643 T6642 compound junction,complexes
R4028 T6644 T6615 punct .,determined
R4029 T6688 T6687 nummod 1c,1f
R403 T851 T852 det The,cause
R4030 T6646 T6647 nmod ZO,expression
R4031 T6647 T6654 nsubj expression,revealed
R4032 T6689 T6687 punct ",",1f
R4033 T6648 T6646 punct -,ZO
R4034 T6649 T6646 nummod 1,ZO
R4035 T6650 T6646 cc and,ZO
R4036 T6690 T6687 nummod 1d,1f
R4037 T6651 T6652 compound F,actin
R4038 T6652 T6646 conj actin,ZO
R4039 T6653 T6652 punct -,actin
R404 T852 T853 nsubj cause,appears
R4040 T6691 T6687 punct ",",1f
R4041 T6655 T6656 det a,layer
R4042 T6656 T6654 dobj layer,revealed
R4043 T6692 T6687 nummod 1e,1f
R4044 T6657 T6656 amod continuous,layer
R4045 T6658 T6656 prep of,layer
R4046 T6659 T6660 amod basal,cells
R4047 T6693 T6687 punct ",",1f
R4048 T6660 T6658 pobj cells,of
R4049 T6661 T6656 punct ", ",layer
R405 T854 T852 prep of,cause
R4050 T6662 T6656 prep including,layer
R4051 T6694 T6685 punct ",",7
R4052 T6663 T6664 det a,junction
R4053 T6664 T6662 pobj junction,including
R4054 T6665 T6664 prep of,junction
R4055 T6695 T6685 nummod 6,7
R4056 T6666 T6667 amod basal,cells
R4057 T6667 T6665 pobj cells,of
R4058 T6696 T6685 punct ",",7
R4059 T6697 T6685 punct ),7
R406 T855 T854 pobj goiter,of
R4060 T6698 T6654 punct .,revealed
R4061 T6700 T6701 det These,observations
R4062 T6774 T6772 dobj concentration,maintain
R4063 T6701 T6702 nsubj observations,make
R4064 T6775 T6774 nmod K,concentration
R4065 T6703 T6704 nsubj it,unlikely
R4066 T6776 T6775 punct +,K
R4067 T6777 T6765 punct ", ",be
R4068 T6778 T6765 advmod however,be
R4069 T6779 T6765 punct ", ",be
R407 T856 T857 aux to,be
R4070 T6780 T6765 aux may,be
R4071 T6781 T6765 acomp less,be
R4072 T6782 T6781 prep than,less
R4073 T6704 T6702 ccomp unlikely,make
R4074 T6783 T6782 amod necessary,than
R4075 T6784 T6765 prep in,be
R4076 T6785 T6786 nmod Slc26a4,mice
R4077 T6705 T6706 mark that,is
R4078 T6786 T6784 pobj mice,in
R4079 T6787 T6785 punct +,Slc26a4
R408 T857 T853 xcomp be,appears
R4080 T6788 T6785 punct /,Slc26a4
R4081 T6706 T6704 advcl is,unlikely
R4082 T6789 T6785 punct +,Slc26a4
R4083 T6790 T6765 prep given,be
R4084 T6791 T6792 det the,numbers
R4085 T6792 T6790 pobj numbers,given
R4086 T6707 T6708 det the,cause
R4087 T6793 T6792 amod reduced,numbers
R4088 T6794 T6792 prep of,numbers
R4089 T6708 T6706 nsubj cause,is
R409 T858 T859 det an,impairment
R4090 T6795 T6796 amod sensory,cells
R4091 T6796 T6794 pobj cells,of
R4092 T6797 T6796 compound hair,cells
R4093 T6709 T6708 amod primary,cause
R4094 T6798 T6796 punct ", ",cells
R4095 T6799 T6800 dep which,provide
R4096 T6710 T6708 prep of,cause
R4097 T6800 T6796 relcl provide,cells
R4098 T6711 T6712 det the,loss
R4099 T6801 T6802 det a,pathway
R410 T859 T857 attr impairment,be
R4100 T6802 T6800 dobj pathway,provide
R4101 T6803 T6802 amod major,pathway
R4102 T6712 T6710 pobj loss,of
R4103 T6804 T6802 prep for,pathway
R4104 T6805 T6806 nmod K,exit
R4105 T6806 T6804 pobj exit,for
R4106 T6807 T6805 punct +,K
R4107 T6808 T6806 prep from,exit
R4108 T6713 T6712 prep of,loss
R4109 T6809 T6808 pobj endolymph,from
R411 T860 T859 prep of,impairment
R4110 T6810 T6811 punct [,31
R4111 T6811 T6765 parataxis 31,be
R4112 T6714 T6715 det the,potential
R4113 T6812 T6811 nummod 30,31
R4114 T6813 T6811 punct ",",31
R4115 T6814 T6811 punct ],31
R4116 T6715 T6713 pobj potential,of
R4117 T6815 T6765 punct .,be
R4118 T6817 T6818 prep In,measured
R4119 T6716 T6715 amod endocochlear,potential
R412 T861 T862 compound iodide,fixation
R4120 T6819 T6817 pobj order,In
R4121 T6820 T6821 aux to,substantiate
R4122 T6821 T6819 acl substantiate,order
R4123 T6822 T6823 det the,view
R4124 T6823 T6821 dobj view,substantiate
R4125 T6717 T6718 det a,compromise
R4126 T6824 T6825 mark that,secretes
R4127 T6825 T6823 acl secretes,view
R4128 T6826 T6827 compound stria,vascularis
R4129 T6827 T6825 nsubj vascularis,secretes
R413 T862 T860 pobj fixation,of
R4130 T6718 T6706 attr compromise,is
R4131 T6828 T6827 prep in,vascularis
R4132 T6719 T6718 prep in,compromise
R4133 T6829 T6828 pobj Slc26a4,in
R4134 T6830 T6829 punct -,Slc26a4
R4135 T6831 T6829 punct /,Slc26a4
R4136 T6720 T6721 det the,barrier
R4137 T6832 T6829 punct -,Slc26a4
R4138 T6833 T6825 dobj K,secretes
R4139 T6834 T6833 punct +,K
R414 T863 T859 prep in,impairment
R4140 T6721 T6719 pobj barrier,in
R4141 T6835 T6818 punct ", ",measured
R4142 T6836 T6818 nsubj we,measured
R4143 T6837 T6838 det the,magnitude
R4144 T6722 T6723 amod basal,cell
R4145 T6838 T6818 dobj magnitude,measured
R4146 T6839 T6838 prep of,magnitude
R4147 T6840 T6841 det the,current
R4148 T6723 T6721 compound cell,barrier
R4149 T6841 T6839 pobj current,of
R415 T864 T865 det the,lumen
R4150 T6842 T6843 npadvmod bumetanide,sensitive
R4151 T6843 T6841 amod sensitive,current
R4152 T6724 T6702 punct .,make
R4153 T6844 T6843 punct -,sensitive
R4154 T6845 T6841 acl exiting,current
R4155 T6846 T6847 compound stria,vascularis
R4156 T6726 T6727 det The,observation
R4157 T6847 T6845 dobj vascularis,exiting
R4158 T6848 T6845 prep across,exiting
R4159 T6849 T6850 det the,membrane
R416 T865 T863 pobj lumen,in
R4160 T6850 T6848 pobj membrane,across
R4161 T6727 T6728 nsubj observation,suggests
R4162 T6851 T6850 amod apical,membrane
R4163 T6852 T6850 prep of,membrane
R4164 T6853 T6854 amod strial,cells
R4165 T6729 T6730 mark that,were
R4166 T6854 T6852 pobj cells,of
R4167 T6855 T6854 amod marginal,cells
R4168 T6856 T6818 cc and,measured
R4169 T6857 T6818 conj determined,measured
R417 T866 T865 amod follicular,lumen
R4170 T6730 T6727 acl were,observation
R4171 T6858 T6859 det the,expression
R4172 T6859 T6857 dobj expression,determined
R4173 T6860 T6859 prep of,expression
R4174 T6861 T6862 det the,proteins
R4175 T6862 T6860 pobj proteins,of
R4176 T6863 T6862 appos KCNQ1,proteins
R4177 T6731 T6732 amod endolymphatic,concentrations
R4178 T6864 T6863 punct ", ",KCNQ1
R4179 T6865 T6863 conj KCNE1,KCNQ1
R418 T867 T857 prep due,be
R4180 T6866 T6865 cc and,KCNE1
R4181 T6732 T6730 nsubj concentrations,were
R4182 T6867 T6865 conj SLC12A2,KCNE1
R4183 T6868 T6862 punct ", ",proteins
R4184 T6869 T6870 dep which,are
R4185 T6733 T6731 cc and,endolymphatic
R4186 T6870 T6862 relcl are,proteins
R4187 T6871 T6870 acomp essential,are
R4188 T6872 T6871 prep for,essential
R4189 T6734 T6731 conj perilymphatic,endolymphatic
R419 T868 T867 pcomp to,due
R4190 T6873 T6874 nmod K,secretion
R4191 T6874 T6872 pobj secretion,for
R4192 T6875 T6873 punct +,K
R4193 T6735 T6732 nmod K,concentrations
R4194 T6876 T6877 punct [,33
R4195 T6877 T6870 parataxis 33,are
R4196 T6878 T6877 nummod 20,33
R4197 T6736 T6735 punct +,K
R4198 T6879 T6877 punct ",",33
R4199 T6737 T6730 acomp normal,were
R420 T869 T870 det a,rate
R4200 T6738 T6730 prep in,were
R4201 T6880 T6877 nummod 32,33
R4202 T6881 T6877 punct ",",33
R4203 T6739 T6740 nmod Slc26a4,mice
R4204 T6882 T6877 punct ],33
R4205 T6740 T6738 pobj mice,in
R4206 T6883 T6860 punct ", ",of
R4207 T6741 T6739 punct -,Slc26a4
R4208 T6884 T6860 cc and,of
R4209 T6885 T6860 conj of,of
R421 T870 T867 pobj rate,due
R4210 T6886 T6887 nmod GJB2,Cx26
R4211 T6742 T6739 punct /,Slc26a4
R4212 T6887 T6885 pobj Cx26,of
R4213 T6888 T6887 punct (,Cx26
R4214 T6743 T6739 punct -,Slc26a4
R4215 T6889 T6887 punct ),Cx26
R4216 T6890 T6887 punct ", ",Cx26
R4217 T6891 T6892 dep which,thought
R4218 T6744 T6745 mark that,was
R4219 T6892 T6887 relcl thought,Cx26
R422 T871 T870 amod reduced,rate
R4220 T6893 T6892 auxpass is,thought
R4221 T6745 T6728 ccomp was,suggests
R4222 T6894 T6895 aux to,contribute
R4223 T6895 T6892 xcomp contribute,thought
R4224 T6746 T6747 compound stria,vascularis
R4225 T6896 T6895 prep to,contribute
R4226 T6897 T6898 nmod K,cycling
R4227 T6898 T6896 pobj cycling,to
R4228 T6899 T6897 punct +,K
R4229 T6900 T6901 punct [,23
R423 T872 T870 prep of,rate
R4230 T6901 T6857 parataxis 23,determined
R4231 T6747 T6745 nsubj vascularis,was
R4232 T6902 T6901 punct ],23
R4233 T6903 T6818 punct .,measured
R4234 T6748 T6745 acomp able,was
R4235 T6905 T6906 nmod K,secretion
R4236 T6906 T6908 nsubjpass secretion,known
R4237 T6907 T6905 punct +,K
R4238 T6749 T6750 aux to,secrete
R4239 T6909 T6908 auxpass is,known
R424 T873 T874 compound iodide,transport
R4240 T6750 T6748 xcomp secrete,able
R4241 T6910 T6911 aux to,be
R4242 T6911 T6908 xcomp be,known
R4243 T6751 T6750 dobj K,secrete
R4244 T6912 T6911 acomp sensitive,be
R4245 T6913 T6912 prep to,sensitive
R4246 T6914 T6915 compound 10,5
R4247 T6752 T6751 punct +,K
R4248 T6915 T6917 nummod 5,M
R4249 T6916 T6915 punct -,5
R425 T874 T872 pobj transport,of
R4250 T6917 T6918 compound M,bumetanide
R4251 T6753 T6745 prep in,was
R4252 T6918 T6913 pobj bumetanide,to
R4253 T6919 T6918 punct ", ",bumetanide
R4254 T6920 T6921 det a,diuretic
R4255 T6754 T6753 pobj spite,in
R4256 T6921 T6918 appos diuretic,bumetanide
R4257 T6922 T6921 compound loop,diuretic
R4258 T6923 T6921 punct -,diuretic
R4259 T6755 T6754 prep of,spite
R426 T875 T874 prep across,transport
R4260 T6924 T6925 dep that,inhibits
R4261 T6925 T6921 relcl inhibits,diuretic
R4262 T6926 T6927 det the,cotransporter
R4263 T6756 T6757 det the,signs
R4264 T6927 T6925 dobj cotransporter,inhibits
R4265 T6928 T6927 nmod Na,cotransporter
R4266 T6757 T6755 pobj signs,of
R4267 T6929 T6928 punct +,Na
R4268 T6930 T6928 punct /,Na
R4269 T6931 T6928 appos 2Cl,Na
R427 T876 T877 det the,membrane
R4270 T6758 T6757 amod apparent,signs
R4271 T6932 T6931 punct -,2Cl
R4272 T6933 T6928 punct /,Na
R4273 T6934 T6928 appos K,Na
R4274 T6759 T6757 prep of,signs
R4275 T6935 T6934 punct +,K
R4276 T6936 T6927 appos SLC12A2,cotransporter
R4277 T6937 T6938 punct [,26
R4278 T6938 T6925 parataxis 26,inhibits
R4279 T6939 T6938 punct ],26
R428 T877 T875 pobj membrane,across
R4280 T6940 T6908 punct .,known
R4281 T6760 T6759 pobj degeneration,of
R4282 T6942 T6943 npadvmod Bumetanide,sensitive
R4283 T6943 T6945 amod sensitive,currents
R4284 T6761 T6728 punct .,suggests
R4285 T6944 T6943 punct -,sensitive
R4286 T6945 T6946 nsubjpass currents,found
R4287 T6763 T6764 det The,rate
R4288 T6947 T6946 auxpass were,found
R4289 T6948 T6946 prep in,found
R429 T878 T877 amod apical,membrane
R4290 T6949 T6950 preconj both,Slc26a4
R4291 T6950 T6951 nmod Slc26a4,mice
R4292 T6764 T6765 nsubj rate,be
R4293 T6951 T6948 pobj mice,in
R4294 T6952 T6950 punct +,Slc26a4
R4295 T6953 T6950 punct /,Slc26a4
R4296 T6766 T6764 prep of,rate
R4297 T6954 T6950 punct +,Slc26a4
R4298 T6955 T6950 cc and,Slc26a4
R4299 T6956 T6950 conj Slc26a4,Slc26a4
R430 T879 T877 prep of,membrane
R4300 T6957 T6956 punct -,Slc26a4
R4301 T6767 T6768 nmod K,secretion
R4302 T6958 T6956 punct /,Slc26a4
R4303 T6959 T6956 punct -,Slc26a4
R4304 T6960 T6961 mark although,was
R4305 T6768 T6766 pobj secretion,of
R4306 T6961 T6946 advcl was,found
R4307 T6962 T6963 det the,magnitude
R4308 T6963 T6961 nsubj magnitude,was
R4309 T6769 T6767 punct +,K
R431 T880 T881 amod thyroid,gland
R4310 T6964 T6963 prep of,magnitude
R4311 T6965 T6966 det the,current
R4312 T6966 T6964 pobj current,of
R4313 T6770 T6764 amod necessary,rate
R4314 T6967 T6968 advmod significantly,smaller
R4315 T6771 T6772 aux to,maintain
R4316 T6968 T6961 acomp smaller,was
R4317 T6969 T6961 prep in,was
R4318 T6772 T6770 xcomp maintain,necessary
R4319 T6970 T6971 nmod Slc26a4,mice
R432 T881 T882 nmod gland,cells
R4320 T6971 T6969 pobj mice,in
R4321 T6773 T6774 amod endolymphatic,concentration
R4322 T6972 T6970 punct -,Slc26a4
R4323 T6973 T6970 punct /,Slc26a4
R4324 T6974 T6970 punct -,Slc26a4
R4325 T6985 T6976 punct =,4
R4326 T6975 T6976 punct (,4
R4327 T6976 T6961 parataxis 4,was
R4328 T6977 T6978 quantmod 22,6
R4329 T6978 T6980 nummod 6,μA
R433 T882 T879 pobj cells,of
R4330 T6979 T6978 punct ±,6
R4331 T6980 T6976 dep μA,4
R4332 T6981 T6982 punct /,cm2
R4333 T6982 T6980 prep cm2,μA
R4334 T6983 T6976 punct ", ",4
R4335 T6984 T6976 nsubj n,4
R4336 T6986 T6976 cc versus,4
R4337 T6987 T6988 quantmod 14,2
R4338 T6988 T6990 nummod 2,μA
R4339 T7091 T7092 amod vestibular,cells
R434 T883 T882 amod epithelial,cells
R4340 T6989 T6988 punct ±,2
R4341 T6990 T6991 dep μA,4
R4342 T6991 T6976 conj 4,4
R4343 T7092 T7090 pobj cells,in
R4344 T6992 T6993 punct /,cm2
R4345 T7093 T7092 amod dark,cells
R4346 T7094 T7095 punct (,Fig.
R4347 T6993 T6990 prep cm2,μA
R4348 T7095 T7082 parataxis Fig.,observed
R4349 T7096 T7095 nummod 1i,Fig.
R435 T884 T885 punct [,4
R4350 T6994 T6991 punct ", ",4
R4351 T7097 T7095 punct ),Fig.
R4352 T7098 T7082 punct .,observed
R4353 T6995 T6991 nsubj n,4
R4354 T7100 T7101 nsubj Expression,is
R4355 T7102 T7100 prep of,Expression
R4356 T6996 T6991 punct =,4
R4357 T7103 T7104 compound KCNQ1,protein
R4358 T7104 T7102 pobj protein,of
R4359 T7105 T7100 prep in,Expression
R436 T885 T857 parataxis 4,be
R4360 T6997 T6991 punct ),4
R4361 T7106 T7107 compound stria,vascularis
R4362 T7107 T7105 pobj vascularis,in
R4363 T6998 T6946 punct .,found
R4364 T7108 T7107 prep of,vascularis
R4365 T7109 T7110 nmod Slc26a4,mice
R4366 T7110 T7108 pobj mice,of
R4367 T7000 T7001 nsubjpass KCNQ1,co-localized
R4368 T7111 T7109 punct -,Slc26a4
R4369 T7112 T7109 punct /,Slc26a4
R437 T886 T885 punct ],4
R4370 T7113 T7109 punct -,Slc26a4
R4371 T7002 T7000 cc and,KCNQ1
R4372 T7114 T7101 acomp consistent,is
R4373 T7115 T7114 prep with,consistent
R4374 T7116 T7117 det the,finding
R4375 T7117 T7115 pobj finding,with
R4376 T7003 T7000 conj KCNE1,KCNQ1
R4377 T7118 T7117 prep of,finding
R4378 T7119 T7120 compound KCNQ1,mRNA
R4379 T7004 T7000 punct ", ",KCNQ1
R438 T887 T853 punct .,appears
R4380 T7120 T7121 compound mRNA,expression
R4381 T7121 T7118 pobj expression,of
R4382 T7122 T7123 punct (,Fig.
R4383 T7123 T7101 parataxis Fig.,is
R4384 T7124 T7123 nummod 3,Fig.
R4385 T7125 T7123 punct ),Fig.
R4386 T7005 T7000 appos subunits,KCNQ1
R4387 T7126 T7101 punct .,is
R4388 T7128 T7129 det These,observations
R4389 T7006 T7005 prep of,subunits
R439 T889 T890 det A,test
R4390 T7129 T7130 nsubj observations,make
R4391 T7131 T7132 nsubj it,unlikely
R4392 T7007 T7008 det the,channel
R4393 T7132 T7130 ccomp unlikely,make
R4394 T7133 T7134 mark that,is
R4395 T7008 T7006 pobj channel,of
R4396 T7009 T7008 amod secretory,channel
R4397 T7134 T7132 advcl is,unlikely
R4398 T7010 T7008 nmod K,channel
R4399 T7135 T7136 det the,cause
R440 T890 T894 nsubj test,appear
R4400 T7136 T7134 nsubj cause,is
R4401 T7137 T7136 amod primary,cause
R4402 T7011 T7010 punct +,K
R4403 T7138 T7136 prep of,cause
R4404 T7139 T7140 det the,loss
R4405 T7012 T7001 punct ", ",co-localized
R4406 T7140 T7138 pobj loss,of
R4407 T7141 T7140 prep of,loss
R4408 T7142 T7143 det the,potential
R4409 T7013 T7001 auxpass were,co-localized
R441 T891 T890 amod positive,test
R4410 T7143 T7141 pobj potential,of
R4411 T7144 T7143 amod endocochlear,potential
R4412 T7145 T7146 det a,compromise
R4413 T7014 T7001 prep in,co-localized
R4414 T7146 T7134 attr compromise,is
R4415 T7147 T7146 prep of,compromise
R4416 T7015 T7016 det the,membrane
R4417 T7148 T7149 nmod K,secretion
R4418 T7149 T7147 pobj secretion,of
R4419 T7016 T7014 pobj membrane,in
R442 T892 T893 compound perchlorate,discharge
R4420 T7150 T7148 punct +,K
R4421 T7151 T7146 prep by,compromise
R4422 T7152 T7153 amod strial,marginal
R4423 T7153 T7154 amod marginal,cells
R4424 T7017 T7016 amod apical,membrane
R4425 T7154 T7151 pobj cells,by
R4426 T7155 T7146 cc or,compromise
R4427 T7018 T7016 prep of,membrane
R4428 T7156 T7157 det a,compromise
R4429 T7157 T7146 conj compromise,compromise
R443 T893 T890 compound discharge,test
R4430 T7158 T7157 prep of,compromise
R4431 T7019 T7020 amod strial,marginal
R4432 T7159 T7160 compound gap,junction
R4433 T7160 T7161 npadvmod junction,mediated
R4434 T7161 T7162 amod mediated,cycling
R4435 T7162 T7158 pobj cycling,of
R4436 T7163 T7162 nmod K,cycling
R4437 T7164 T7163 punct +,K
R4438 T7020 T7021 amod marginal,cells
R4439 T7165 T7130 punct .,make
R444 T895 T890 cc and,test
R4440 T7021 T7018 pobj cells,of
R4441 T7022 T7001 punct ;,co-localized
R4442 T7023 T7024 det the,cotransporter
R4443 T7024 T7033 nsubjpass cotransporter,found
R4444 T7025 T7024 nmod Na,cotransporter
R4445 T7026 T7025 punct +,Na
R4446 T7027 T7025 punct /,Na
R4447 T7028 T7025 appos 2Cl,Na
R4448 T7029 T7028 punct -,2Cl
R4449 T7030 T7025 punct /,Na
R445 T896 T897 det an,aqueduct
R4450 T7031 T7025 appos K,Na
R4451 T7032 T7031 punct +,K
R4452 T7033 T7001 conj found,co-localized
R4453 T7034 T7024 appos SLC12A2,cotransporter
R4454 T7035 T7024 punct ", ",cotransporter
R4455 T7036 T7037 dep which,located
R4456 T7037 T7024 relcl located,cotransporter
R4457 T7038 T7037 auxpass is,located
R4458 T7039 T7037 prep in,located
R4459 T7040 T7041 det the,membrane
R446 T897 T890 conj aqueduct,test
R4460 T7041 T7039 pobj membrane,in
R4461 T7042 T7041 amod basolateral,membrane
R4462 T7043 T7041 prep of,membrane
R4463 T7044 T7045 amod strial,cells
R4464 T7045 T7043 pobj cells,of
R4465 T7046 T7045 amod marginal,cells
R4466 T7047 T7033 punct ", ",found
R4467 T7048 T7033 auxpass was,found
R4468 T7049 T7033 prep in,found
R4469 T7050 T7051 compound stria,vascularis
R447 T898 T897 amod enlarged,aqueduct
R4470 T7051 T7049 pobj vascularis,in
R4471 T7052 T7033 punct ;,found
R4472 T7053 T7033 cc and,found
R4473 T7054 T7055 det the,protein
R4474 T7055 T7058 nsubjpass protein,found
R4475 T7056 T7057 compound gap,junction
R4476 T7057 T7055 compound junction,protein
R4477 T7058 T7033 conj found,found
R4478 T7059 T7055 appos GJB2,protein
R4479 T7060 T7058 auxpass was,found
R448 T899 T897 amod vestibular,aqueduct
R4480 T7061 T7058 prep in,found
R4481 T7062 T7063 amod spiral,ligament
R4482 T7063 T7061 pobj ligament,in
R4483 T7064 T7063 prep of,ligament
R4484 T7065 T7066 nmod Slc26a4,mice
R4485 T7066 T7064 pobj mice,of
R4486 T7067 T7065 punct +,Slc26a4
R4487 T7068 T7065 punct /,Slc26a4
R4488 T7069 T7065 punct +,Slc26a4
R4489 T7070 T7065 cc and,Slc26a4
R449 T900 T901 aux to,be
R4490 T7071 T7065 conj Slc26a4,Slc26a4
R4491 T7072 T7071 punct -,Slc26a4
R4492 T7073 T7071 punct /,Slc26a4
R4493 T7074 T7071 punct -,Slc26a4
R4494 T7075 T7076 punct (,Fig.
R4495 T7076 T7058 parataxis Fig.,found
R4496 T7077 T7076 nummod 8,Fig.
R4497 T7078 T7076 punct ),Fig.
R4498 T7079 T7058 punct .,found
R4499 T7081 T7082 nsubjpass Co-localization,observed
R450 T901 T894 xcomp be,appear
R4500 T7083 T7081 prep of,Co-localization
R4501 T7084 T7085 nmod KCNQ1,proteins
R4502 T7085 T7083 pobj proteins,of
R4503 T7086 T7084 cc and,KCNQ1
R4504 T7087 T7084 conj KCNE1,KCNQ1
R4505 T7088 T7082 auxpass was,observed
R4506 T7089 T7082 advmod also,observed
R4507 T7090 T7082 prep in,observed
R451 T902 T903 det the,signs
R4510 T7393 T7394 nsubj We,described
R4511 T7395 T7394 dobj locations,described
R4512 T7396 T7395 prep of,locations
R4513 T7397 T7398 compound pendrin,expression
R4514 T7398 T7396 pobj expression,of
R4515 T7399 T7395 prep in,locations
R4516 T7400 T7401 det the,cochlea
R4517 T7401 T7399 pobj cochlea,in
R4518 T7402 T7401 cc and,cochlea
R4519 T7403 T7404 amod vestibular,labyrinth
R452 T903 T901 attr signs,be
R4520 T7404 T7401 conj labyrinth,cochlea
R4521 T7405 T7394 cc and,described
R4522 T7406 T7394 conj detected,described
R4523 T7407 T7408 amod normal,concentrations
R4524 T7408 T7406 dobj concentrations,detected
R4525 T7409 T7408 amod endolymphatic,concentrations
R4526 T7410 T7408 nmod K,concentrations
R4527 T7411 T7410 punct +,K
R4528 T7412 T7406 prep in,detected
R4529 T7413 T7412 pobj spite,in
R453 T904 T905 advmod most,reliable
R4530 T7414 T7413 prep of,spite
R4531 T7415 T7416 det an,enlargement
R4532 T7416 T7414 pobj enlargement,of
R4533 T7417 T7416 prep of,enlargement
R4534 T7418 T7419 det this,compartment
R4535 T7419 T7417 pobj compartment,of
R4536 T7420 T7419 compound fluid,compartment
R4537 T7421 T7394 punct .,described
R4538 T7423 T7424 nsubj We,found
R4539 T7425 T7426 mark that,lack
R454 T905 T903 amod reliable,signs
R4540 T7426 T7424 ccomp lack,found
R4541 T7427 T7428 nmod Slc26a4,mice
R4542 T7428 T7426 nsubj mice,lack
R4543 T7429 T7427 punct -,Slc26a4
R4544 T7430 T7427 punct /,Slc26a4
R4545 T7431 T7427 punct -,Slc26a4
R4546 T7432 T7433 det the,potential
R4547 T7433 T7426 dobj potential,lack
R4548 T7434 T7433 amod endocochlear,potential
R4549 T7435 T7436 mark because,express
R455 T906 T903 amod clinical,signs
R4550 T7436 T7426 advcl express,lack
R4551 T7437 T7436 nsubj they,express
R4552 T7438 T7436 aux do,express
R4553 T7439 T7436 neg not,express
R4554 T7440 T7441 compound KCNJ10,protein
R4555 T7441 T7436 dobj protein,express
R4556 T7442 T7424 punct .,found
R4557 T7444 T7445 amod Intermediate,cells
R4558 T7445 T7446 nsubj cells,protect
R4559 T7447 T7448 compound stria,vascularis
R456 T907 T903 prep of,signs
R4560 T7448 T7446 dobj vascularis,protect
R4561 T7449 T7446 prep by,protect
R4562 T7450 T7449 pcomp converting,by
R4563 T7451 T7450 dobj CO2,converting
R4564 T7452 T7450 prep to,converting
R4565 T7453 T7452 pobj HCO3,to
R4566 T7454 T7453 punct -,HCO3
R4567 T7455 T7449 cc and,by
R4568 T7456 T7449 conj by,by
R4569 T7457 T7456 pcomp detoxifying,by
R457 T908 T909 compound Pendred,syndrome
R4570 T7458 T7459 amod free,radicals
R4571 T7459 T7457 dobj radicals,detoxifying
R4572 T7460 T7461 punct (,Fig.
R4573 T7461 T7446 parataxis Fig.,protect
R4574 T7462 T7461 nummod 9,Fig.
R4575 T7463 T7461 punct ),Fig.
R4576 T7464 T7446 punct .,protect
R4577 T7466 T7467 nsubjpass CO2,generated
R4578 T7468 T7466 cc and,CO2
R4579 T7469 T7470 amod free,radicals
R458 T909 T907 pobj syndrome,of
R4580 T7470 T7466 conj radicals,CO2
R4581 T7471 T7467 auxpass are,generated
R4582 T7472 T7467 agent by,generated
R4583 T7473 T7474 det the,numbers
R4584 T7474 T7472 pobj numbers,by
R4585 T7475 T7474 amod large,numbers
R4586 T7476 T7474 prep of,numbers
R4587 T7477 T7476 pobj mitochondria,of
R4588 T7478 T7474 prep in,numbers
R4589 T7479 T7480 det the,cells
R459 T910 T911 punct [,5
R4590 T7480 T7478 pobj cells,in
R4591 T7481 T7482 advmod metabolically,active
R4592 T7482 T7480 amod active,cells
R4593 T7483 T7482 advmod highly,active
R4594 T7484 T7485 amod strial,marginal
R4595 T7485 T7480 amod marginal,cells
R4596 T7486 T7467 punct .,generated
R4597 T7488 T7489 amod Intermediate,cells
R4598 T7489 T7490 nsubj cells,employ
R4599 T7491 T7492 amod carbonic,anhydrase
R460 T911 T901 parataxis 5,be
R4600 T7492 T7490 dobj anhydrase,employ
R4601 T7493 T7494 aux to,convert
R4602 T7494 T7490 advcl convert,employ
R4603 T7495 T7494 dobj CO2,convert
R4604 T7496 T7494 prep to,convert
R4605 T7497 T7496 pobj HCO3,to
R4606 T7498 T7497 punct -,HCO3
R4607 T7499 T7500 punct [,19
R4608 T7500 T7494 parataxis 19,convert
R4609 T7501 T7500 nummod 18,19
R461 T912 T911 punct ],5
R4610 T7502 T7500 punct ",",19
R4611 T7503 T7500 punct ],19
R4612 T7504 T7490 cc and,employ
R4613 T7505 T7490 conj catalase,employ
R4614 T7506 T7507 aux to,detoxify
R4615 T7507 T7505 advcl detoxify,catalase
R4616 T7508 T7509 amod free,radicals
R4617 T7509 T7507 dobj radicals,detoxify
R4618 T7510 T7490 punct .,employ
R4619 T7512 T7513 aux To,protect
R462 T913 T894 punct .,appear
R4620 T7513 T7514 advcl protect,generate
R4621 T7515 T7513 dobj themselves,protect
R4622 T7516 T7513 prep from,protect
R4623 T7517 T7518 amod free,damage
R4624 T7518 T7516 pobj damage,from
R4625 T7519 T7518 compound radical,damage
R4626 T7520 T7514 punct ", ",generate
R4627 T7521 T7522 amod intermediate,cells
R4628 T7522 T7514 nsubj cells,generate
R4629 T7523 T7514 dobj glutathione,generate
R463 T915 T916 nsubj Pendrin,is
R4630 T7524 T7523 cc and,glutathione
R4631 T7525 T7526 compound melanin,pigment
R4632 T7526 T7523 conj pigment,glutathione
R4633 T7527 T7528 punct [,34
R4634 T7528 T7514 parataxis 34,generate
R4635 T7529 T7530 punct -,37
R4636 T7530 T7528 prep 37,34
R4637 T7531 T7528 punct ],34
R4638 T7532 T7514 punct .,generate
R4639 T7534 T7535 nsubj It,is
R464 T917 T918 det an,exchanger
R4640 T7536 T7535 acomp conceivable,is
R4641 T7537 T7538 mark that,results
R4642 T7538 T7535 ccomp results,is
R4643 T7539 T7538 nsubj loss,results
R4644 T7540 T7539 prep of,loss
R4645 T7541 T7540 pobj pendrin,of
R4646 T7542 T7541 punct ", ",pendrin
R4647 T7543 T7544 dep which,secrete
R4648 T7544 T7541 relcl secrete,pendrin
R4649 T7545 T7544 aux may,secrete
R465 T918 T916 attr exchanger,is
R4650 T7546 T7544 dobj HCO3,secrete
R4651 T7547 T7546 punct -,HCO3
R4652 T7548 T7544 prep into,secrete
R4653 T7549 T7548 pobj endolymph,into
R4654 T7550 T7538 punct ", ",results
R4655 T7551 T7538 prep in,results
R4656 T7552 T7553 det an,accumulation
R4657 T7553 T7551 pobj accumulation,in
R4658 T7554 T7553 prep of,accumulation
R4659 T7555 T7554 pobj HCO3,of
R466 T919 T918 compound anion,exchanger
R4660 T7556 T7555 punct -,HCO3
R4661 T7557 T7553 cc and,accumulation
R4662 T7558 T7559 det an,alkalinization
R4663 T7559 T7553 conj alkalinization,accumulation
R4664 T7560 T7559 prep of,alkalinization
R4665 T7561 T7562 det the,spaces
R4666 T7562 T7560 pobj spaces,of
R4667 T7563 T7562 amod intrastrial,spaces
R4668 T7564 T7535 punct .,is
R4669 T7566 T7567 det This,alkalinization
R467 T920 T921 dep that,transport
R4670 T7567 T7569 nsubj alkalinization,enhance
R4671 T7568 T7567 amod extracellular,alkalinization
R4672 T7570 T7569 aux may,enhance
R4673 T7571 T7572 amod free,radical
R4674 T7572 T7573 compound radical,stress
R4675 T7573 T7569 dobj stress,enhance
R4676 T7574 T7569 punct ", ",enhance
R4677 T7575 T7576 mark since,inhibit
R4678 T7576 T7569 advcl inhibit,enhance
R4679 T7577 T7576 nsubj it,inhibit
R468 T921 T918 relcl transport,exchanger
R4680 T7578 T7576 aux may,inhibit
R4681 T7579 T7580 det the,uptake
R4682 T7580 T7576 dobj uptake,inhibit
R4683 T7581 T7580 prep of,uptake
R4684 T7582 T7581 pobj cysteine,of
R4685 T7583 T7576 cc and,inhibit
R4686 T7584 T7585 advmod thereby,limit
R4687 T7585 T7576 conj limit,inhibit
R4688 T7586 T7585 dobj production,limit
R4689 T7587 T7586 prep of,production
R469 T922 T921 aux can,transport
R4690 T7588 T7589 det the,glutathione
R4691 T7589 T7587 pobj glutathione,of
R4692 T7590 T7589 amod protective,glutathione
R4693 T7591 T7592 punct [,38
R4694 T7592 T7569 parataxis 38,enhance
R4695 T7593 T7592 punct ],38
R4696 T7594 T7569 punct .,enhance
R4697 T7596 T7597 nsubj Support,comes
R4698 T7598 T7596 prep for,Support
R4699 T7599 T7600 det the,hypothesis
R470 T923 T921 dobj Cl,transport
R4700 T7600 T7598 pobj hypothesis,for
R4701 T7601 T7600 prep of,hypothesis
R4702 T7602 T7603 amod enhanced,stress
R4703 T7603 T7601 pobj stress,of
R4704 T7604 T7605 amod free,radical
R4705 T7605 T7603 compound radical,stress
R4706 T7606 T7597 prep from,comes
R4707 T7607 T7608 det the,hyperpigmentation
R4708 T7608 T7606 pobj hyperpigmentation,from
R4709 T7609 T7608 amod observed,hyperpigmentation
R471 T924 T923 punct -,Cl
R4710 T7610 T7608 prep in,hyperpigmentation
R4711 T7611 T7610 pobj mice,in
R4712 T7612 T7611 acl lacking,mice
R4713 T7613 T7612 dobj pendrin,lacking
R4714 T7614 T7597 punct .,comes
R4715 T7616 T7617 amod Strial,hyperpigmentation
R4716 T7617 T7618 nsubjpass hyperpigmentation,observed
R4717 T7619 T7618 aux has,observed
R4718 T7620 T7618 advmod also,observed
R4719 T7621 T7618 auxpass been,observed
R472 T925 T923 punct ", ",Cl
R4720 T7622 T7618 prep in,observed
R4721 T7623 T7624 amod other,conditions
R4722 T7624 T7622 pobj conditions,in
R4723 T7625 T7626 dep that,associated
R4724 T7626 T7624 relcl associated,conditions
R4725 T7627 T7626 auxpass are,associated
R4726 T7628 T7626 prep with,associated
R4727 T7629 T7630 amod free,radical
R4728 T7630 T7631 compound radical,stress
R4729 T7631 T7628 pobj stress,with
R473 T926 T923 conj I,Cl
R4730 T7632 T7624 punct ", ",conditions
R4731 T7633 T7634 amod such,as
R4732 T7634 T7624 prep as,conditions
R4733 T7635 T7636 amod acoustic,trauma
R4734 T7636 T7634 pobj trauma,as
R4735 T7637 T7638 punct [,37
R4736 T7638 T7618 parataxis 37,observed
R4737 T7639 T7638 punct ],37
R4738 T7640 T7618 punct .,observed
R4739 T7642 T7643 nsubj Alterations,lead
R474 T927 T926 punct -,I
R4740 T7644 T7642 prep in,Alterations
R4741 T7645 T7646 det the,pH
R4742 T7646 T7644 pobj pH,in
R4743 T7647 T7646 amod cytosolic,pH
R4744 T7648 T7642 prep in,Alterations
R4745 T7649 T7648 pobj conjunction,in
R4746 T7650 T7649 prep with,conjunction
R4747 T7651 T7652 amod free,radical
R4748 T7652 T7653 compound radical,stress
R4749 T7653 T7650 pobj stress,with
R475 T928 T926 punct ", ",I
R4750 T7654 T7643 aux may,lead
R4751 T7655 T7643 prep to,lead
R4752 T7656 T7657 det the,loss
R4753 T7657 T7655 pobj loss,to
R4754 T7658 T7657 prep of,loss
R4755 T7659 T7660 compound KCNJ10,expression
R4756 T7660 T7658 pobj expression,of
R4757 T7661 T7660 compound protein,expression
R4758 T7662 T7643 prep in,lead
R4759 T7663 T7664 amod strial,cells
R476 T929 T926 conj HCO3,I
R4760 T7664 T7662 pobj cells,in
R4761 T7665 T7664 amod intermediate,cells
R4762 T7666 T7643 punct .,lead
R4763 T7668 T7669 nsubjpass Function,shown
R4764 T7670 T7668 cc and,Function
R4765 T7671 T7668 conj expression,Function
R4766 T7672 T7668 prep of,Function
R4767 T7673 T7674 amod other,channels
R4768 T7674 T7672 pobj channels,of
R4769 T7675 T7674 nmod K,channels
R477 T930 T929 punct -,HCO3
R4770 T7676 T7675 punct +,K
R4771 T7677 T7669 aux has,shown
R4772 T7678 T7669 auxpass been,shown
R4773 T7679 T7680 aux to,controlled
R4774 T7680 T7669 xcomp controlled,shown
R4775 T7681 T7680 auxpass be,controlled
R4776 T7682 T7680 agent by,controlled
R4777 T7683 T7684 det the,pH
R4778 T7684 T7682 pobj pH,by
R4779 T7685 T7684 amod cytosolic,pH
R478 T931 T929 cc and,HCO3
R4780 T7686 T7684 cc and,pH
R4781 T7687 T7688 amod free,radicals
R4782 T7688 T7684 conj radicals,pH
R4783 T7689 T7684 punct ", ",pH
R4784 T7690 T7691 dep which,encode
R4785 T7691 T7684 relcl encode,pH
R4786 T7692 T7693 det the,state
R4787 T7693 T7691 dobj state,encode
R4788 T7694 T7693 amod metabolic,state
R4789 T7695 T7693 prep of,state
R479 T932 T929 conj formate,HCO3
R4790 T7696 T7697 det the,cell
R4791 T7697 T7695 pobj cell,of
R4792 T7698 T7699 punct [,39
R4793 T7699 T7669 parataxis 39,shown
R4794 T7700 T7699 punct ],39
R4795 T7701 T7669 punct .,shown
R4796 T7703 T7704 nsubj Suppression,is
R4797 T7705 T7703 prep of,Suppression
R4798 T7706 T7707 det the,channel
R4799 T7707 T7705 pobj channel,of
R480 T933 T934 punct [,7
R4800 T7708 T7707 nmod KCNJ10,channel
R4801 T7709 T7707 nmod K,channel
R4802 T7710 T7709 punct +,K
R4803 T7711 T7703 prep in,Suppression
R4804 T7712 T7713 amod strial,cells
R4805 T7713 T7711 pobj cells,in
R4806 T7714 T7713 amod intermediate,cells
R4807 T7715 T7703 punct ", ",Suppression
R4808 T7716 T7717 dep which,is
R4809 T7717 T7703 relcl is,Suppression
R481 T934 T921 parataxis 7,transport
R4810 T7718 T7717 acomp essential,is
R4811 T7719 T7718 prep for,essential
R4812 T7720 T7721 det the,generation
R4813 T7721 T7719 pobj generation,for
R4814 T7722 T7721 prep of,generation
R4815 T7723 T7724 det the,potential
R4816 T7724 T7722 pobj potential,of
R4817 T7725 T7724 amod endocochlear,potential
R4818 T7726 T7704 punct ", ",is
R4819 T7727 T7704 advmod probably,is
R482 T935 T934 nummod 6,7
R4820 T7728 T7729 det the,cause
R4821 T7729 T7704 attr cause,is
R4822 T7730 T7729 amod direct,cause
R4823 T7731 T7729 prep of,cause
R4824 T7732 T7731 pobj deafness,of
R4825 T7733 T7729 prep in,cause
R4826 T7734 T7735 nmod Slc26a4,mice
R4827 T7735 T7733 pobj mice,in
R4828 T7736 T7734 punct -,Slc26a4
R4829 T7737 T7734 punct /,Slc26a4
R483 T936 T934 punct ",",7
R4830 T7738 T7734 punct -,Slc26a4
R4831 T7739 T7735 cc and,mice
R4832 T7740 T7735 conj patients,mice
R4833 T7741 T7740 acl suffering,patients
R4834 T7742 T7741 prep from,suffering
R4835 T7743 T7744 compound Pendred,syndrome
R4836 T7744 T7742 pobj syndrome,from
R4837 T7745 T7704 punct .,is
R4838 T7746 T7747 det The,history
R4839 T7747 T7749 nsubjpass history,accessed
R484 T937 T934 punct ],7
R4840 T7748 T7747 amod pre-publication,history
R4841 T7750 T7747 prep for,history
R4842 T7751 T7752 det this,paper
R4843 T7752 T7750 pobj paper,for
R4844 T7753 T7749 aux can,accessed
R4845 T7754 T7749 auxpass be,accessed
R4846 T7755 T7749 advmod here,accessed
R4847 T7756 T7749 punct :,accessed
R485 T938 T916 punct .,is
R4850 T7896 T7897 compound Protein,localization
R4851 T7898 T7897 prep of,localization
R4852 T7899 T7898 pobj pendrin,of
R4853 T7900 T7899 punct ", ",pendrin
R4854 T7901 T7899 conj KCNQ1,pendrin
R4855 T7902 T7901 punct ", ",KCNQ1
R4856 T7903 T7901 conj ZO,KCNQ1
R4857 T7904 T7903 punct -,ZO
R4858 T7905 T7903 nummod 1,ZO
R4859 T7906 T7903 cc and,ZO
R486 T940 T941 nsubjpass Expression,found
R4860 T7907 T7908 compound F,actin
R4861 T7908 T7903 conj actin,ZO
R4862 T7909 T7897 prep in,localization
R4863 T7910 T7909 pobj cochlea,in
R4864 T7911 T7910 cc and,cochlea
R4865 T7912 T7913 amod vestibular,labyrinth
R4866 T7913 T7910 conj labyrinth,cochlea
R4867 T7914 T7910 prep of,cochlea
R4868 T7915 T7916 nmod Slc26a4,mice
R4869 T7916 T7914 pobj mice,of
R487 T942 T941 aux has,found
R4870 T7917 T7915 punct +,Slc26a4
R4871 T7918 T7915 punct /,Slc26a4
R4872 T7919 T7915 punct +,Slc26a4
R4873 T7920 T7915 cc and,Slc26a4
R4874 T7921 T7915 conj Slc26a4,Slc26a4
R4875 T7922 T7921 punct -,Slc26a4
R4876 T7923 T7921 punct /,Slc26a4
R4877 T7924 T7921 punct -,Slc26a4
R4878 T7925 T7897 punct .,localization
R4879 T7927 T7928 meta a,μm
R488 T943 T941 auxpass been,found
R4880 T7929 T7928 punct : ,μm
R4881 T7930 T7928 dep Overview,μm
R4882 T7931 T7930 prep of,Overview
R4883 T7932 T7931 pobj cochlea,of
R4884 T7933 T7928 punct ;,μm
R4885 T7934 T7928 nsubj bar,μm
R4886 T7935 T7928 punct =,μm
R4887 T7936 T7928 nummod 100,μm
R4888 T7937 T7928 punct .,μm
R4889 T7939 T7940 meta b,μm
R489 T944 T941 prep in,found
R4890 T7941 T7939 punct -,b
R4891 T7942 T7939 dep f,b
R4892 T7943 T7940 punct : ,μm
R4893 T7944 T7940 dep Detail,μm
R4894 T7945 T7944 prep of,Detail
R4895 T7946 T7947 amod cochlear,wall
R4896 T7947 T7945 pobj wall,of
R4897 T7948 T7947 amod lateral,wall
R4898 T7949 T7940 punct ;,μm
R4899 T7950 T7940 nsubj bar,μm
R490 T945 T946 det the,ear
R4900 T7951 T7940 punct =,μm
R4901 T7952 T7940 nummod 10,μm
R4902 T7953 T7940 punct .,μm
R4903 T7955 T7956 meta g,μm
R4904 T7957 T7956 punct : ,μm
R4905 T7958 T7956 dep Detail,μm
R4906 T7959 T7958 prep of,Detail
R4907 T7960 T7959 pobj utricle,of
R4908 T7961 T7956 punct ;,μm
R4909 T7962 T7956 nsubj bar,μm
R491 T946 T944 pobj ear,in
R4910 T7963 T7956 punct =,μm
R4911 T7964 T7956 nummod 10,μm
R4912 T7965 T7956 punct .,μm
R4913 T7967 T7968 meta h,μm
R4914 T7969 T7967 punct -,h
R4915 T7970 T7967 dep i,h
R4916 T7971 T7968 punct : ,μm
R4917 T7972 T7968 dep Detail,μm
R4918 T7973 T7972 prep of,Detail
R4919 T7974 T7973 pobj ampulla,of
R492 T947 T946 amod inner,ear
R4920 T7975 T7968 punct ;,μm
R4921 T7976 T7968 nsubj bar,μm
R4922 T7977 T7968 punct =,μm
R4923 T7978 T7968 nummod 10,μm
R4924 T7979 T7968 punct .,μm
R4925 T7982 T7981 punct ", ",RM
R4926 T7983 T7984 poss Reissner,membrane
R4927 T7984 T7981 appos membrane,RM
R4928 T7985 T7983 case 's,Reissner
R4929 T7986 T7981 punct ;,RM
R493 T948 T946 cc and,ear
R4930 T7987 T7981 appos SC,RM
R4931 T7988 T7987 punct ", ",SC
R4932 T7989 T7990 npadvmod spindle,shaped
R4933 T7990 T7992 amod shaped,cells
R4934 T7991 T7990 punct -,shaped
R4935 T7992 T7987 appos cells,SC
R4936 T7993 T7981 punct ", ",RM
R4937 T7994 T7981 appos SMC,RM
R4938 T7995 T7994 punct ", ",SMC
R4939 T7996 T7997 amod strial,marginal
R494 T949 T950 compound thyroid,gland
R4940 T7997 T7998 amod marginal,cells
R4941 T7998 T7994 appos cells,SMC
R4942 T7999 T7981 punct ;,RM
R4943 T8000 T7981 appos SV,RM
R4944 T8001 T8000 punct ", ",SV
R4945 T8002 T8003 compound stria,vascularis
R4946 T8003 T8000 appos vascularis,SV
R4947 T8004 T7981 punct ;,RM
R4948 T8005 T7981 appos SL,RM
R4949 T8006 T8005 punct ", ",SL
R495 T950 T946 conj gland,ear
R4950 T8007 T8008 amod spiral,ligament
R4951 T8008 T8005 appos ligament,SL
R4952 T8009 T7981 punct ;,RM
R4953 T8010 T7981 appos LIM,RM
R4954 T8011 T8010 punct ", ",LIM
R4955 T8012 T8013 amod spiral,limbus
R4956 T8013 T8010 appos limbus,LIM
R4957 T8014 T7981 punct .,RM
R4958 T8017 T8016 punct ", ",BC
R4959 T8018 T8019 amod basal,cells
R496 T951 T941 advcl consistent,found
R4960 T8019 T8016 appos cells,BC
R4961 T8020 T8016 punct ;,BC
R4962 T8021 T8016 appos SP,BC
R4963 T8022 T8021 punct ", ",SP
R4964 T8023 T8024 amod spiral,prominence
R4965 T8024 T8025 nmod prominence,cells
R4966 T8025 T8021 appos cells,SP
R4967 T8026 T8025 amod epithelial,cells
R4968 T8027 T8016 punct ;,BC
R4969 T8028 T8016 appos RC,BC
R497 T952 T951 prep with,consistent
R4970 T8029 T8028 punct ", ",RC
R4971 T8030 T8031 compound root,cells
R4972 T8031 T8028 appos cells,RC
R4973 T8032 T8016 punct ;,BC
R4974 T8033 T8016 appos OS,BC
R4975 T8034 T8033 punct ", ",OS
R4976 T8035 T8036 amod outer,sulcus
R4977 T8036 T8037 nmod sulcus,cells
R4978 T8037 T8033 appos cells,OS
R4979 T8038 T8037 amod epithelial,cells
R498 T953 T954 det the,signs
R4980 T8039 T8016 punct ;,BC
R4981 T8040 T8016 appos VHC,BC
R4982 T8041 T8040 punct ", ",VHC
R4983 T8042 T8043 amod vestibular,cells
R4984 T8043 T8040 appos cells,VHC
R4985 T8044 T8043 compound hair,cells
R4986 T8045 T8016 punct ;,BC
R4987 T8046 T8016 appos VTC,BC
R4988 T8047 T8046 punct ", ",VTC
R4989 T8048 T8049 amod vestibular,cells
R499 T954 T952 pobj signs,with
R4990 T8049 T8046 appos cells,VTC
R4991 T8050 T8049 amod transitional,cells
R4992 T8051 T8016 punct ;,BC
R4993 T8052 T8016 appos VDC,BC
R4994 T8053 T8052 punct ", ",VDC
R4995 T8054 T8055 amod vestibular,cells
R4996 T8055 T8052 appos cells,VDC
R4997 T8056 T8055 amod dark,cells
R4998 T8057 T8016 punct ;,BC
R4999 T8058 T8059 dep arrows,form
R500 T955 T954 amod clinical,signs
R5000 T8059 T8016 dep form,BC
R5001 T8060 T8059 punct ", ",form
R5002 T8061 T8062 amod basal,cells
R5003 T8062 T8059 nsubj cells,form
R5004 T8063 T8062 prep at,cells
R5005 T8064 T8065 det the,top
R5006 T8065 T8063 pobj top,at
R5007 T8066 T8065 cc and,top
R5008 T8067 T8065 conj bottom,top
R5009 T8068 T8065 prep of,top
R501 T956 T954 prep of,signs
R5010 T8069 T8070 compound stria,vascularis
R5011 T8070 T8068 pobj vascularis,of
R5012 T8071 T8072 amod tight,junctions
R5013 T8072 T8059 dobj junctions,form
R5014 T8073 T8072 prep with,junctions
R5015 T8074 T8075 nmod surface,cells
R5016 T8075 T8073 pobj cells,with
R5017 T8076 T8075 amod epithelial,cells
R5018 T8077 T8016 punct .,BC
R502 T957 T956 pobj deafness,of
R503 T958 T957 cc and,deafness
R5033 T8160 T8159 punct ", ",Potential
R5034 T8161 T8162 nmod K,concentrations
R5035 T8162 T8159 conj concentrations,Potential
R5036 T8163 T8161 punct +,K
R5037 T8164 T8162 cc and,concentrations
R5038 T8165 T8162 conj pigmentation,concentrations
R5039 T8166 T8165 prep of,pigmentation
R504 T959 T957 conj goiter,deafness
R5040 T8167 T8168 compound stria,vascularis
R5041 T8168 T8166 pobj vascularis,of
R5042 T8169 T8159 prep in,Potential
R5043 T8170 T8171 nmod Slc26a4,mice
R5044 T8171 T8169 pobj mice,in
R5045 T8172 T8170 punct +,Slc26a4
R5046 T8173 T8170 punct /,Slc26a4
R5047 T8174 T8170 punct +,Slc26a4
R5048 T8175 T8170 cc and,Slc26a4
R5049 T8176 T8170 conj Slc26a4,Slc26a4
R505 T960 T961 punct [,8
R5050 T8177 T8176 punct -,Slc26a4
R5051 T8178 T8176 punct /,Slc26a4
R5052 T8179 T8176 punct -,Slc26a4
R5053 T8180 T8159 punct .,Potential
R5054 T8182 T8183 meta a,potential
R5055 T8184 T8183 punct : ,potential
R5056 T8185 T8183 amod Endocochlear,potential
R5057 T8186 T8183 cc and,potential
R5058 T8187 T8188 nmod K,concentrations
R5059 T8188 T8183 conj concentrations,potential
R506 T961 T941 parataxis 8,found
R5060 T8189 T8187 punct +,K
R5061 T8190 T8183 prep in,potential
R5062 T8191 T8190 pobj endolymph,in
R5063 T8192 T8191 cc and,endolymph
R5064 T8193 T8191 conj perilymph,endolymph
R5065 T8194 T8183 prep at,potential
R5066 T8195 T8196 det the,apex
R5067 T8196 T8194 pobj apex,at
R5068 T8197 T8198 punct (,A
R5069 T8198 T8196 parataxis A,apex
R507 T962 T961 nummod 2,8
R5070 T8199 T8198 punct ),A
R5071 T8200 T8196 cc and,apex
R5072 T8201 T8196 conj base,apex
R5073 T8202 T8203 punct (,B
R5074 T8203 T8201 parataxis B,base
R5075 T8204 T8203 punct ),B
R5076 T8205 T8196 prep of,apex
R5077 T8206 T8207 det the,cochlea
R5078 T8207 T8205 pobj cochlea,of
R5079 T8208 T8183 punct .,potential
R508 T963 T961 punct ",",8
R5080 T8210 T8211 nsubj Numbers,denote
R5081 T8212 T8210 amod adjacent,Numbers
R5082 T8213 T8212 prep to,adjacent
R5083 T8214 T8213 pobj symbols,to
R5084 T8215 T8211 dobj number,denote
R5085 T8216 T8215 prep of,number
R5086 T8217 T8216 pobj measurements,of
R5087 T8218 T8211 punct .,denote
R5088 T8220 T8221 meta b,Pigmentation
R5089 T8222 T8220 punct -,b
R509 T964 T961 nummod 3,8
R5090 T8223 T8220 dep d,b
R5091 T8224 T8221 punct : ,Pigmentation
R5092 T8225 T8221 prep of,Pigmentation
R5093 T8226 T8227 compound stria,vascularis
R5094 T8227 T8225 pobj vascularis,of
R5095 T8228 T8221 prep in,Pigmentation
R5096 T8229 T8230 nmod Slc26a4,mice
R5097 T8230 T8228 pobj mice,in
R5098 T8231 T8229 punct +,Slc26a4
R5099 T8232 T8229 punct /,Slc26a4
R510 T965 T961 punct ",",8
R5100 T8233 T8229 punct +,Slc26a4
R5101 T8234 T8229 cc and,Slc26a4
R5102 T8235 T8229 conj Slc26a4,Slc26a4
R5103 T8236 T8235 punct -,Slc26a4
R5104 T8237 T8235 punct /,Slc26a4
R5105 T8238 T8235 punct -,Slc26a4
R5106 T8239 T8221 punct .,Pigmentation
R5107 T8241 T8242 meta b,View
R5108 T8243 T8242 punct : ,View
R5109 T8244 T8242 prep of,View
R511 T966 T961 punct ],8
R5110 T8245 T8246 compound stria,vascularis
R5111 T8246 T8244 pobj vascularis,of
R5112 T8247 T8242 prep through,View
R5113 T8248 T8249 det the,capsule
R5114 T8249 T8247 pobj capsule,through
R5115 T8250 T8249 amod bony,capsule
R5116 T8251 T8249 prep of,capsule
R5117 T8252 T8253 det the,cochlea
R5118 T8253 T8251 pobj cochlea,of
R5119 T8254 T8242 punct .,View
R512 T967 T941 punct .,found
R5120 T8257 T8256 punct ", ",OW
R5121 T8258 T8259 amod oval,window
R5122 T8259 T8256 appos window,OW
R5123 T8260 T8256 punct ", ",OW
R5124 T8261 T8256 appos RW,OW
R5125 T8262 T8261 punct ", ",RW
R5126 T8263 T8264 amod round,window
R5127 T8264 T8261 appos window,RW
R5128 T8265 T8256 punct ;,OW
R5129 T8266 T8256 appos arrows,OW
R513 T969 T970 prep In,found
R5130 T8267 T8266 punct ", ",arrows
R5131 T8268 T8269 compound stria,vascularis
R5132 T8269 T8266 appos vascularis,arrows
R5133 T8270 T8256 punct .,OW
R5134 T8272 T8273 meta c,mounts
R5135 T8274 T8272 punct -,c
R5136 T8275 T8272 dep d,c
R5137 T8276 T8273 punct : ,mounts
R5138 T8277 T8273 amod Whole,mounts
R5139 T8278 T8273 punct -,mounts
R514 T971 T969 pobj addition,In
R5140 T8279 T8273 prep of,mounts
R5141 T8280 T8281 compound stria,vascularis
R5142 T8281 T8279 pobj vascularis,of
R5143 T8282 T8273 acl isolated,mounts
R5144 T8283 T8282 prep from,isolated
R5145 T8284 T8285 npadvmod age,matched
R5146 T8285 T8287 amod matched,mice
R5147 T8286 T8285 punct -,matched
R5148 T8287 T8283 pobj mice,from
R5149 T8288 T8273 punct .,mounts
R515 T972 T970 punct ", ",found
R5150 T8290 T8291 meta c,μm
R5151 T8291 T8301 dep μm,Quantification
R5152 T8292 T8291 punct : ,μm
R5153 T8293 T8294 nmod Laser,images
R5154 T8294 T8291 dep images,μm
R5155 T8295 T8293 punct -,Laser
R5156 T8296 T8293 amod scanning,Laser
R5157 T8297 T8291 punct ", ",μm
R5158 T8298 T8291 nsubj bar,μm
R5159 T8299 T8291 punct =,μm
R516 T973 T974 compound pendrin,expression
R5160 T8300 T8291 nummod 10,μm
R5161 T8302 T8301 punct ", ",Quantification
R5162 T8303 T8301 meta d,Quantification
R5163 T8304 T8301 punct : ,Quantification
R5164 T8305 T8301 prep of,Quantification
R5165 T8306 T8305 pobj pigmentation,of
R5166 T8307 T8301 prep based,Quantification
R5167 T8308 T8307 prep on,based
R5168 T8309 T8310 amod optical,density
R5169 T8310 T8308 pobj density,on
R517 T974 T970 nsubjpass expression,found
R5170 T8311 T8301 punct .,Quantification
R5173 T8436 T8435 prep of,Quantification
R5174 T8437 T8438 nmod KCNJ10,expression
R5175 T8438 T8436 pobj expression,of
R5176 T8439 T8437 cc and,KCNJ10
R5177 T8440 T8437 conj KCNQ1,KCNJ10
R5178 T8441 T8438 compound mRNA,expression
R5179 T8442 T8438 prep in,expression
R518 T975 T970 aux has,found
R5180 T8443 T8444 compound stria,vascularis
R5181 T8444 T8442 pobj vascularis,in
R5182 T8445 T8444 cc and,vascularis
R5183 T8446 T8447 amod spiral,ganglia
R5184 T8447 T8444 conj ganglia,vascularis
R5185 T8448 T8444 prep of,vascularis
R5186 T8449 T8450 nmod Slc26a4,mice
R5187 T8450 T8448 pobj mice,of
R5188 T8451 T8449 punct +,Slc26a4
R5189 T8452 T8449 punct /,Slc26a4
R519 T976 T970 auxpass been,found
R5190 T8453 T8449 punct +,Slc26a4
R5191 T8454 T8449 cc and,Slc26a4
R5192 T8455 T8449 conj Slc26a4,Slc26a4
R5193 T8456 T8455 punct -,Slc26a4
R5194 T8457 T8455 punct /,Slc26a4
R5195 T8458 T8455 punct -,Slc26a4
R5196 T8459 T8435 punct .,Quantification
R5197 T8461 T8462 meta a,Electropherogram
R5198 T8463 T8462 punct : ,Electropherogram
R5199 T8464 T8462 prep of,Electropherogram
R520 T977 T970 prep in,found
R5200 T8465 T8466 amod total,RNA
R5201 T8466 T8464 pobj RNA,of
R5202 T8467 T8466 acl isolated,RNA
R5203 T8468 T8467 prep from,isolated
R5204 T8469 T8470 compound stria,vascularis
R5205 T8470 T8468 pobj vascularis,from
R5206 T8471 T8470 acl microdissected,vascularis
R5207 T8472 T8471 prep from,microdissected
R5208 T8473 T8474 nummod one,mouse
R5209 T8474 T8472 pobj mouse,from
R521 T978 T979 det the,kidney
R5210 T8475 T8462 punct .,Electropherogram
R5211 T8477 T8478 det The,amount
R5212 T8478 T8479 nsubjpass amount,obtained
R5213 T8480 T8478 prep of,amount
R5214 T8481 T8482 amod total,RNA
R5215 T8482 T8480 pobj RNA,of
R5216 T8483 T8479 auxpass was,obtained
R5217 T8484 T8479 prep from,obtained
R5218 T8485 T8486 det the,integral
R5219 T8486 T8484 pobj integral,from
R522 T979 T977 pobj kidney,in
R5220 T8487 T8486 amod total,integral
R5221 T8488 T8489 punct (,shaded
R5222 T8489 T8486 parataxis shaded,integral
R5223 T8490 T8489 punct ),shaded
R5224 T8491 T8479 cc and,obtained
R5225 T8492 T8493 det the,amount
R5226 T8493 T8494 nsubjpass amount,obtained
R5227 T8494 T8479 conj obtained,obtained
R5228 T8495 T8493 prep of,amount
R5229 T8496 T8497 compound 18S,rRNA
R523 T980 T981 punct [,9
R5230 T8497 T8495 pobj rRNA,of
R5231 T8498 T8494 auxpass was,obtained
R5232 T8499 T8494 prep from,obtained
R5233 T8500 T8501 det the,integral
R5234 T8501 T8499 pobj integral,from
R5235 T8502 T8501 prep of,integral
R5236 T8503 T8504 det the,peak
R5237 T8504 T8502 pobj peak,of
R5238 T8505 T8504 compound 18S,peak
R5239 T8506 T8494 punct .,obtained
R524 T981 T979 parataxis 9,kidney
R5240 T8508 T8509 amod Sharp,peaks
R5241 T8509 T8510 nsubj peaks,demonstrate
R5242 T8511 T8509 acl representing,peaks
R5243 T8512 T8513 nmod 18S,rRNA
R5244 T8513 T8511 dobj rRNA,representing
R5245 T8514 T8512 cc and,18S
R5246 T8515 T8512 conj 28S,18S
R5247 T8516 T8517 det the,quality
R5248 T8517 T8510 dobj quality,demonstrate
R5249 T8518 T8517 amod high,quality
R525 T982 T981 punct ],9
R5250 T8519 T8517 prep of,quality
R5251 T8520 T8519 pobj RNA,of
R5252 T8521 T8510 punct .,demonstrate
R5253 T8523 T8524 dep Insert,verified
R5254 T8525 T8524 punct : ,verified
R5255 T8526 T8524 nsubjpass Genotype,verified
R5256 T8527 T8526 prep of,Genotype
R5257 T8528 T8529 nmod Slc26a4,mice
R5258 T8529 T8527 pobj mice,of
R5259 T8530 T8528 punct -,Slc26a4
R526 T983 T979 punct ", ",kidney
R5260 T8531 T8528 punct /,Slc26a4
R5261 T8532 T8528 punct -,Slc26a4
R5262 T8533 T8524 auxpass was,verified
R5263 T8534 T8524 prep by,verified
R5264 T8535 T8536 det the,observation
R5265 T8536 T8534 pobj observation,by
R5266 T8537 T8536 prep of,observation
R5267 T8538 T8539 nummod one,otoconia
R5268 T8539 T8537 pobj otoconia,of
R5269 T8540 T8538 cc or,one
R527 T984 T985 amod mammary,gland
R5270 T8541 T8538 conj few,one
R5271 T8542 T8543 advmod very,large
R5272 T8543 T8539 amod large,otoconia
R5273 T8544 T8539 amod rhomboedric,otoconia
R5274 T8545 T8539 prep in,otoconia
R5275 T8546 T8547 det the,macula
R5276 T8547 T8545 pobj macula,in
R5277 T8548 T8547 amod utricular,macula
R5278 T8549 T8550 punct (,arrow
R5279 T8550 T8524 parataxis arrow,verified
R528 T985 T979 conj gland,kidney
R5280 T8551 T8550 punct ),arrow
R5281 T8552 T8524 punct .,verified
R5282 T8555 T8554 punct ", ",A
R5283 T8556 T8557 compound crista,ampullaris
R5284 T8557 T8554 appos ampullaris,A
R5285 T8558 T8554 punct ;,A
R5286 T8559 T8554 appos U,A
R5287 T8560 T8559 punct ", ",U
R5288 T8561 T8562 amod utricular,macula
R5289 T8562 T8559 appos macula,U
R529 T986 T987 punct [,10
R5290 T8563 T8554 punct .,A
R5291 T8565 T8566 compound Scale,bar
R5292 T8566 T8567 dep bar,μm
R5293 T8568 T8567 punct : ,μm
R5294 T8569 T8567 nummod 100,μm
R5295 T8570 T8567 punct .,μm
R5296 T8572 T8573 meta b,Example
R5297 T8574 T8573 punct : ,Example
R5298 T8575 T8573 prep of,Example
R5299 T8576 T8577 amod real,time
R530 T987 T985 parataxis 10,gland
R5300 T8577 T8579 compound time,data
R5301 T8578 T8577 punct -,time
R5302 T8579 T8575 pobj data,of
R5303 T8580 T8581 compound RT,PCR
R5304 T8581 T8579 compound PCR,data
R5305 T8582 T8581 punct -,PCR
R5306 T8583 T8579 acl used,data
R5307 T8584 T8583 prep for,used
R5308 T8585 T8584 pobj quantification,for
R5309 T8586 T8585 prep of,quantification
R531 T988 T987 punct ],10
R5310 T8587 T8586 pobj 18S,of
R5311 T8588 T8587 punct ", ",18S
R5312 T8589 T8587 conj KCNJ10,18S
R5313 T8590 T8589 punct ", ",KCNJ10
R5314 T8591 T8589 conj KCNQ1,KCNJ10
R5315 T8592 T8591 cc and,KCNQ1
R5316 T8593 T8591 conj KCNQ4,KCNQ1
R5317 T8594 T8573 punct .,Example
R5318 T8596 T8597 amod Known,quantities
R5319 T8597 T8598 nsubjpass quantities,used
R532 T989 T985 punct ", ",gland
R5320 T8599 T8597 prep of,quantities
R5321 T8600 T8601 compound 18S,rRNA
R5322 T8601 T8599 pobj rRNA,of
R5323 T8602 T8598 auxpass were,used
R5324 T8603 T8604 aux to,calibrate
R5325 T8604 T8598 advcl calibrate,used
R5326 T8605 T8606 det the,threshold
R5327 T8606 T8604 dobj threshold,calibrate
R5328 T8607 T8598 punct .,used
R5329 T8610 T8609 punct ", ",SV
R533 T990 T985 conj uterus,gland
R5330 T8611 T8612 compound stria,vascularis
R5331 T8612 T8609 appos vascularis,SV
R5332 T8613 T8609 punct ;,SV
R5333 T8614 T8609 appos SG,SV
R5334 T8615 T8614 punct ", ",SG
R5335 T8616 T8617 amod spiral,ganglia
R5336 T8617 T8614 appos ganglia,SG
R5337 T8618 T8609 punct .,SV
R5338 T8620 T8621 meta c,Quantification
R5339 T8622 T8621 punct : ,Quantification
R534 T991 T992 punct [,11
R5340 T8623 T8621 prep of,Quantification
R5341 T8624 T8625 nmod KCNJ10,mRNA
R5342 T8625 T8623 pobj mRNA,of
R5343 T8626 T8624 cc and,KCNJ10
R5344 T8627 T8624 conj KCNQ1,KCNJ10
R5345 T8628 T8621 prep in,Quantification
R5346 T8629 T8630 amod young,Slc26a4
R5347 T8630 T8628 pobj Slc26a4,in
R5348 T8631 T8630 punct +,Slc26a4
R5349 T8632 T8630 punct /,Slc26a4
R535 T992 T990 parataxis 11,uterus
R5350 T8633 T8630 punct +,Slc26a4
R5351 T8634 T8630 cc and,Slc26a4
R5352 T8635 T8636 amod young,-
R5353 T8636 T8642 punct -,mice
R5354 T8637 T8635 cc and,young
R5355 T8638 T8635 conj old,young
R5356 T8639 T8636 nmod Slc26a4,-
R5357 T8640 T8636 punct -,-
R5358 T8641 T8636 punct /,-
R5359 T8642 T8630 conj mice,Slc26a4
R536 T993 T992 punct ],11
R5360 T8643 T8621 punct .,Quantification
R5361 T8717 T8718 compound Protein,localization
R5362 T8719 T8718 prep of,localization
R5363 T8720 T8719 pobj KCNJ10,of
R5364 T8721 T8718 prep in,localization
R5365 T8722 T8723 det the,cochlea
R5366 T8723 T8721 pobj cochlea,in
R5367 T8724 T8723 prep of,cochlea
R5368 T8725 T8726 nmod Slc26a4,mice
R5369 T8726 T8724 pobj mice,of
R537 T994 T990 punct ", ",uterus
R5370 T8727 T8725 punct +,Slc26a4
R5371 T8728 T8725 punct /,Slc26a4
R5372 T8729 T8725 punct +,Slc26a4
R5373 T8730 T8725 cc and,Slc26a4
R5374 T8731 T8725 conj Slc26a4,Slc26a4
R5375 T8732 T8731 punct -,Slc26a4
R5376 T8733 T8731 punct /,Slc26a4
R5377 T8734 T8731 punct -,Slc26a4
R5378 T8735 T8718 punct .,localization
R5379 T8737 T8738 meta a,μm
R538 T995 T990 conj testes,uterus
R5380 T8739 T8738 punct : ,μm
R5381 T8740 T8738 dep Overview,μm
R5382 T8741 T8740 prep of,Overview
R5383 T8742 T8741 pobj cochlea,of
R5384 T8743 T8738 punct ;,μm
R5385 T8744 T8738 nsubj bar,μm
R5386 T8745 T8738 punct =,μm
R5387 T8746 T8738 nummod 100,μm
R5388 T8747 T8738 punct .,μm
R5389 T8750 T8749 prep to,Compare
R539 T996 T997 punct [,12
R5390 T8751 T8750 pobj Fig.,to
R5391 T8752 T8751 nummod 1a,Fig.
R5392 T8753 T8754 aux to,note
R5393 T8754 T8749 advcl note,Compare
R5394 T8755 T8756 det the,media
R5395 T8756 T8754 dobj media,note
R5396 T8757 T8756 amod enlarged,media
R5397 T8758 T8756 compound scala,media
R5398 T8759 T8756 cc and,media
R5399 T8760 T8761 det the,membrane
R540 T997 T995 parataxis 12,testes
R5400 T8761 T8756 conj membrane,media
R5401 T8762 T8761 amod distended,membrane
R5402 T8763 T8761 poss Reissner,membrane
R5403 T8764 T8763 case 's,Reissner
R5404 T8765 T8749 punct .,Compare
R5405 T8767 T8768 meta b,Detail
R5406 T8769 T8767 punct -,b
R5407 T8770 T8767 dep c,b
R5408 T8771 T8768 punct : ,Detail
R5409 T8772 T8768 prep of,Detail
R541 T998 T997 punct ],12
R5410 T8773 T8774 amod lateral,wall
R5411 T8774 T8772 pobj wall,of
R5412 T8775 T8774 cc and,wall
R5413 T8776 T8777 amod spiral,ganglia
R5414 T8777 T8774 conj ganglia,wall
R5415 T8778 T8779 punct (,insert
R5416 T8779 T8768 parataxis insert,Detail
R5417 T8780 T8779 punct ),insert
R5418 T8781 T8768 punct ;,Detail
R5419 T8782 T8783 amod main,bar
R542 T999 T995 cc and,testes
R5420 T8783 T8784 nsubj bar,μm
R5421 T8784 T8768 dep μm,Detail
R5422 T8785 T8784 punct : ,μm
R5423 T8786 T8784 nummod 10,μm
R5424 T8787 T8768 punct ", ",Detail
R5425 T8788 T8789 nsubj insert,μm
R5426 T8789 T8768 dep μm,Detail
R5427 T8790 T8789 punct : ,μm
R5428 T8791 T8789 nummod 5,μm
R5429 T8792 T8768 punct .,Detail
R543 T1000 T995 conj placenta,testes
R5430 T8794 T8795 nsubj Expression,was
R5431 T8796 T8794 prep of,Expression
R5432 T8797 T8796 pobj KCNJ10,of
R5433 T8798 T8794 prep in,Expression
R5434 T8799 T8800 nmod Slc26a4,mice
R5435 T8800 T8798 pobj mice,in
R5436 T8801 T8799 punct -,Slc26a4
R5437 T8802 T8799 punct /,Slc26a4
R5438 T8803 T8799 punct -,Slc26a4
R5439 T8804 T8795 acomp absent,was
R544 T1001 T1002 punct [,13
R5440 T8805 T8804 prep in,absent
R5441 T8806 T8807 compound stria,vascularis
R5442 T8807 T8805 pobj vascularis,in
R5443 T8808 T8804 cc but,absent
R5444 T8809 T8804 conj unchanged,absent
R5445 T8810 T8809 prep in,unchanged
R5446 T8811 T8812 amod spiral,ganglion
R5447 T8812 T8813 compound ganglion,cells
R5448 T8813 T8810 pobj cells,in
R5449 T8814 T8795 punct .,was
R545 T1002 T1000 parataxis 13,placenta
R5450 T8817 T8816 punct ", ",RM
R5451 T8818 T8819 poss Reissner,membrane
R5452 T8819 T8816 appos membrane,RM
R5453 T8820 T8818 case 's,Reissner
R5454 T8821 T8816 punct ", ",RM
R5455 T8822 T8816 appos SV,RM
R5456 T8823 T8822 punct ", ",SV
R5457 T8824 T8825 compound stria,vascularis
R5458 T8825 T8822 appos vascularis,SV
R5459 T8826 T8816 punct ;,RM
R546 T1003 T1002 punct ],13
R5460 T8827 T8816 appos SP,RM
R5461 T8828 T8827 punct ", ",SP
R5462 T8829 T8830 amod spiral,prominence
R5463 T8830 T8827 appos prominence,SP
R5464 T8831 T8816 punct ;,RM
R5465 T8832 T8816 appos SL,RM
R5466 T8833 T8832 punct ", ",SL
R5467 T8834 T8835 amod spiral,ligament
R5468 T8835 T8832 appos ligament,SL
R5469 T8836 T8816 punct ;,RM
R547 T1004 T970 punct .,found
R5470 T8837 T8816 appos LIM,RM
R5471 T8838 T8837 punct ", ",LIM
R5472 T8839 T8840 amod spiral,limbus
R5473 T8840 T8837 appos limbus,LIM
R5474 T8841 T8816 punct ;,RM
R5475 T8842 T8816 appos SG,RM
R5476 T8843 T8842 punct ", ",SG
R5477 T8844 T8845 amod spiral,ganglion
R5478 T8845 T8842 appos ganglion,SG
R5479 T8846 T8816 punct .,RM
R548 T1006 T1007 det No,expression
R5484 T8911 T8912 amod Morphometric,analysis
R5485 T8913 T8912 prep of,analysis
R5486 T8914 T8915 amod cochlear,masses
R5487 T8915 T8913 pobj masses,of
R5488 T8916 T8915 compound tissue,masses
R5489 T8917 T8912 prep in,analysis
R549 T1007 T1008 nsubjpass expression,found
R5490 T8918 T8919 nmod Slc26a4,mice
R5491 T8919 T8917 pobj mice,in
R5492 T8920 T8918 punct +,Slc26a4
R5493 T8921 T8918 punct /,Slc26a4
R5494 T8922 T8918 punct +,Slc26a4
R5495 T8923 T8918 cc and,Slc26a4
R5496 T8924 T8918 conj Slc26a4,Slc26a4
R5497 T8925 T8924 punct -,Slc26a4
R5498 T8926 T8924 punct /,Slc26a4
R5499 T8927 T8924 punct -,Slc26a4
R550 T1009 T1008 auxpass was,found
R5500 T8928 T8912 punct .,analysis
R5501 T8930 T8931 meta a,locations
R5502 T8932 T8931 punct : ,locations
R5503 T8933 T8931 prep of,locations
R5504 T8934 T8933 pobj measurements,of
R5505 T8935 T8931 punct .,locations
R5506 T8937 T8938 nsubjpass Thickness,obtained
R5507 T8939 T8937 prep of,Thickness
R5508 T8940 T8941 compound stria,vascularis
R5509 T8941 T8939 pobj vascularis,of
R551 T1010 T1008 prep in,found
R5510 T8942 T8941 punct (,vascularis
R5511 T8943 T8941 appos SV,vascularis
R5512 T8944 T8941 punct ),vascularis
R5513 T8945 T8938 auxpass was,obtained
R5514 T8946 T8938 prep as,obtained
R5515 T8947 T8946 pobj average,as
R5516 T8948 T8947 prep of,average
R5517 T8949 T8950 nummod three,measurements
R5518 T8950 T8948 pobj measurements,of
R5519 T8951 T8950 compound distance,measurements
R552 T1011 T1012 amod fetal,brain
R5520 T8952 T8950 amod perpendicular,measurements
R5521 T8953 T8952 prep to,perpendicular
R5522 T8954 T8955 det the,surface
R5523 T8955 T8953 pobj surface,to
R5524 T8956 T8955 prep of,surface
R5525 T8957 T8958 amod marginal,cells
R5526 T8958 T8956 pobj cells,of
R5527 T8959 T8938 punct .,obtained
R5528 T8961 T8962 nsubjpass Thickness,measured
R5529 T8963 T8961 prep of,Thickness
R553 T1012 T1010 pobj brain,in
R5530 T8964 T8965 amod spiral,prominence
R5531 T8965 T8963 pobj prominence,of
R5532 T8966 T8965 punct (,prominence
R5533 T8967 T8965 appos SP,prominence
R5534 T8968 T8965 punct ),prominence
R5535 T8969 T8962 auxpass was,measured
R5536 T8970 T8962 advcl perpendicular,measured
R5537 T8971 T8970 prep to,perpendicular
R5538 T8972 T8973 det a,line
R5539 T8973 T8971 pobj line,to
R554 T1013 T1011 cc or,fetal
R5540 T8974 T8973 amod tangential,line
R5541 T8975 T8976 punct (,dashed
R5542 T8976 T8973 parataxis dashed,line
R5543 T8977 T8976 punct ),dashed
R5544 T8978 T8979 dep that,connects
R5545 T8979 T8973 relcl connects,line
R5546 T8980 T8981 det the,surface
R5547 T8981 T8979 dobj surface,connects
R5548 T8982 T8981 prep of,surface
R5549 T8983 T8984 det the,sulcus
R555 T1014 T1011 conj adult,fetal
R5550 T8984 T8982 pobj sulcus,of
R5551 T8985 T8984 amod outer,sulcus
R5552 T8986 T8984 punct (,sulcus
R5553 T8987 T8984 appos OS,sulcus
R5554 T8988 T8984 punct ),sulcus
R5555 T8989 T8979 prep with,connects
R5556 T8990 T8991 det the,layer
R5557 T8991 T8989 pobj layer,with
R5558 T8992 T8991 amod basal,layer
R5559 T8993 T8991 prep of,layer
R556 T1015 T1008 punct ", ",found
R5560 T8994 T8995 compound stria,vascularis
R5561 T8995 T8993 pobj vascularis,of
R5562 T8996 T8962 punct .,measured
R5563 T8998 T8999 nsubjpass Thickness,measured
R5564 T9000 T8998 prep of,Thickness
R5565 T9001 T9002 amod spiral,ligament
R5566 T9002 T9000 pobj ligament,of
R5567 T9003 T9002 punct (,ligament
R5568 T9004 T9002 appos SL,ligament
R5569 T9005 T9002 punct ),ligament
R557 T1016 T1008 advcl consistent,found
R5570 T9006 T8999 auxpass was,measured
R5571 T9007 T8999 advcl perpendicular,measured
R5572 T9008 T9007 prep to,perpendicular
R5573 T9009 T9010 det the,line
R5574 T9010 T9008 pobj line,to
R5575 T9011 T9010 amod tangential,line
R5576 T9012 T8999 prep as,measured
R5577 T9013 T9012 pobj distance,as
R5578 T9014 T9013 prep between,distance
R5579 T9015 T9016 det the,surface
R558 T1017 T1016 prep with,consistent
R5580 T9016 T9014 pobj surface,between
R5581 T9017 T9016 prep of,surface
R5582 T9018 T9019 amod spiral,prominence
R5583 T9019 T9017 pobj prominence,of
R5584 T9020 T9016 cc and,surface
R5585 T9021 T9022 det the,interface
R5586 T9022 T9016 conj interface,surface
R5587 T9023 T9022 prep between,interface
R5588 T9024 T9025 amod spiral,ligament
R5589 T9025 T9023 pobj ligament,between
R559 T1018 T1019 det a,cause
R5590 T9026 T9025 cc and,ligament
R5591 T9027 T9025 conj bone,ligament
R5592 T9028 T9027 punct (,bone
R5593 T9029 T9027 appos B,bone
R5594 T9030 T9027 punct ),bone
R5595 T9031 T8999 punct .,measured
R5596 T9033 T9034 nsubjpass Thickness,obtained
R5597 T9035 T9033 prep of,Thickness
R5598 T9036 T9037 amod spiral,limbus
R5599 T9037 T9035 pobj limbus,of
R560 T1019 T1017 pobj cause,with
R5600 T9038 T9037 punct (,limbus
R5601 T9039 T9037 appos LIM,limbus
R5602 T9040 T9037 punct ),limbus
R5603 T9041 T9034 auxpass was,obtained
R5604 T9042 T9034 advcl perpendicular,obtained
R5605 T9043 T9042 prep to,perpendicular
R5606 T9044 T9045 det the,surface
R5607 T9045 T9043 pobj surface,to
R5608 T9046 T9045 prep of,surface
R5609 T9047 T9048 det the,bone
R561 T1020 T1019 amod peripheral,cause
R5610 T9048 T9046 pobj bone,of
R5611 T9049 T9034 prep as,obtained
R5612 T9050 T9051 det a,line
R5613 T9051 T9049 pobj line,as
R5614 T9052 T9051 amod tangential,line
R5615 T9053 T9054 dep that,touches
R5616 T9054 T9051 relcl touches,line
R5617 T9055 T9056 det the,sulcus
R5618 T9056 T9054 dobj sulcus,touches
R5619 T9057 T9056 amod inner,sulcus
R562 T1021 T1019 prep of,cause
R5620 T9058 T9056 punct (,sulcus
R5621 T9059 T9056 appos IS,sulcus
R5622 T9060 T9056 punct ),sulcus
R5623 T9061 T9054 cc and,touches
R5624 T9062 T9054 conj reaches,touches
R5625 T9063 T9062 prep from,reaches
R5626 T9064 T9065 det the,surface
R5627 T9065 T9063 pobj surface,from
R5628 T9066 T9065 prep of,surface
R5629 T9067 T9068 det the,limbus
R563 T1022 T1021 pobj deafness,of
R5630 T9068 T9066 pobj limbus,of
R5631 T9069 T9068 amod spiral,limbus
R5632 T9070 T9062 prep to,reaches
R5633 T9071 T9072 det the,interface
R5634 T9072 T9070 pobj interface,to
R5635 T9073 T9072 prep between,interface
R5636 T9074 T9075 amod spiral,limbus
R5637 T9075 T9073 pobj limbus,between
R5638 T9076 T9077 amod connective,tissue
R5639 T9077 T9075 appos tissue,limbus
R564 T1023 T1024 punct [,11
R5640 T9078 T9077 cc and,tissue
R5641 T9079 T9077 conj bone,tissue
R5642 T9080 T9034 punct .,obtained
R5643 T9082 T9083 meta b,Summary
R5644 T9084 T9083 punct : ,Summary
R5645 T9085 T9083 punct .,Summary
R5646 T9087 T9088 nsubj Data,contributed
R5647 T9089 T9087 prep from,Data
R5648 T9090 T9091 quantmod 7,8
R5649 T9091 T9093 nummod 8,animals
R565 T1024 T1008 parataxis 11,found
R5650 T9092 T9091 punct –,8
R5651 T9093 T9089 pobj animals,from
R5652 T9094 T9088 prep to,contributed
R5653 T9095 T9096 det each,column
R5654 T9096 T9094 pobj column,to
R5655 T9097 T9088 punct .,contributed
R5658 T9177 T9176 prep of,Analysis
R5659 T9178 T9179 amod marginal,cell
R566 T1025 T1024 nummod 2,11
R5660 T9179 T9182 compound cell,barriers
R5661 T9180 T9178 cc and,marginal
R5662 T9181 T9178 conj basal,marginal
R5663 T9182 T9177 pobj barriers,of
R5664 T9183 T9182 nmod by,barriers
R5665 T9184 T9182 prep in,barriers
R5666 T9185 T9186 nmod Slc26a4,mice
R5667 T9186 T9184 pobj mice,in
R5668 T9187 T9185 punct -,Slc26a4
R5669 T9188 T9185 punct /,Slc26a4
R567 T1026 T1024 punct ",",11
R5670 T9189 T9185 punct -,Slc26a4
R5671 T9190 T9176 punct .,Analysis
R5672 T9192 T9193 amod Tight,junctions
R5673 T9193 T9194 nsubjpass junctions,visualized
R5674 T9195 T9194 auxpass were,visualized
R5675 T9196 T9194 prep by,visualized
R5676 T9197 T9198 compound F,actin
R5677 T9198 T9196 pobj actin,by
R5678 T9199 T9194 punct .,visualized
R5679 T9201 T9202 amod Whole,mounts
R568 T1027 T1024 punct ],11
R5680 T9202 T9204 nsubjpass mounts,viewed
R5681 T9203 T9202 punct -,mounts
R5682 T9205 T9202 prep of,mounts
R5683 T9206 T9207 compound stria,vascularis
R5684 T9207 T9205 pobj vascularis,of
R5685 T9208 T9204 auxpass were,viewed
R5686 T9209 T9210 preconj either,from
R5687 T9210 T9204 prep from,viewed
R5688 T9211 T9212 det the,side
R5689 T9212 T9210 pobj side,from
R569 T1028 T1008 punct .,found
R5690 T9213 T9214 amod basal,cell
R5691 T9214 T9212 compound cell,side
R5692 T9215 T9216 punct (,a
R5693 T9216 T9212 parataxis a,side
R5694 T9217 T9218 punct -,f
R5695 T9218 T9216 prep f,a
R5696 T9219 T9216 punct ),a
R5697 T9220 T9210 cc or,from
R5698 T9221 T9210 conj from,from
R5699 T9222 T9223 det the,side
R570 T1030 T1031 nsubjpass Expression,found
R5700 T9223 T9221 pobj side,from
R5701 T9224 T9225 amod marginal,cell
R5702 T9225 T9223 compound cell,side
R5703 T9226 T9227 punct (,g
R5704 T9227 T9223 parataxis g,side
R5705 T9228 T9229 punct -,l
R5706 T9229 T9227 prep l,g
R5707 T9230 T9227 punct ),g
R5708 T9231 T9204 punct .,viewed
R5709 T9233 T9234 amod Bright,field
R571 T1032 T1030 prep of,Expression
R5710 T9234 T9235 compound field,images
R5711 T9235 T9236 nsubj images,verify
R5712 T9237 T9238 mark that,viewed
R5713 T9238 T9236 ccomp viewed,verify
R5714 T9239 T9240 det the,area
R5715 T9240 T9238 nsubjpass area,viewed
R5716 T9241 T9240 amod same,area
R5717 T9242 T9238 auxpass was,viewed
R5718 T9243 T9238 prep from,viewed
R5719 T9244 T9245 preconj either,side
R572 T1033 T1034 compound pendrin,mRNA
R5720 T9245 T9243 pobj side,from
R5721 T9246 T9247 punct (,b
R5722 T9247 T9236 parataxis b,verify
R5723 T9248 T9247 cc and,b
R5724 T9249 T9247 conj h,b
R5725 T9250 T9247 punct ),b
R5726 T9251 T9236 punct .,verify
R5727 T9253 T9254 amod Colored,images
R5728 T9254 T9257 nsubjpass images,mixed
R5729 T9255 T9256 amod bright,field
R573 T1034 T1032 pobj mRNA,of
R5730 T9256 T9254 compound field,images
R5731 T9258 T9257 auxpass were,mixed
R5732 T9259 T9257 prep with,mixed
R5733 T9260 T9259 pobj images,with
R5734 T9261 T9260 prep of,images
R5735 T9262 T9263 compound F,actin
R5736 T9263 T9264 compound actin,staining
R5737 T9264 T9261 pobj staining,of
R5738 T9265 T9266 aux to,indicate
R5739 T9266 T9257 advcl indicate,mixed
R574 T1035 T1030 prep in,Expression
R5740 T9267 T9268 det the,position
R5741 T9268 T9266 dobj position,indicate
R5742 T9269 T9268 prep of,position
R5743 T9270 T9271 compound pigment,granules
R5744 T9271 T9269 pobj granules,of
R5745 T9272 T9273 punct (,d
R5746 T9273 T9257 parataxis d,mixed
R5747 T9274 T9273 punct ", ",d
R5748 T9275 T9273 conj j,d
R5749 T9276 T9275 punct ", ",j
R575 T1036 T1037 det the,ear
R5750 T9277 T9275 conj f,j
R5751 T9278 T9277 cc and,f
R5752 T9279 T9277 conj l,f
R5753 T9280 T9273 punct ),d
R5754 T9281 T9257 punct .,mixed
R5755 T9283 T9284 nsubjpass Focus,varied
R5756 T9285 T9284 auxpass was,varied
R5757 T9286 T9287 aux to,visualize
R5758 T9287 T9284 advcl visualize,varied
R5759 T9288 T9287 preconj either,visualize
R576 T1037 T1035 pobj ear,in
R5760 T9289 T9290 det the,barrier
R5761 T9290 T9287 dobj barrier,visualize
R5762 T9291 T9292 amod marginal,cell
R5763 T9292 T9290 compound cell,barrier
R5764 T9293 T9294 punct (,c
R5765 T9294 T9290 parataxis c,barrier
R5766 T9295 T9294 dep SMC,c
R5767 T9296 T9294 punct ", ",c
R5768 T9297 T9298 punct -,d
R5769 T9298 T9294 prep d,c
R577 T1038 T1037 amod inner,ear
R5770 T9299 T9294 cc and,c
R5771 T9300 T9294 conj i,c
R5772 T9301 T9302 punct -,j
R5773 T9302 T9300 prep j,i
R5774 T9303 T9294 punct ),c
R5775 T9304 T9290 cc or,barrier
R5776 T9305 T9306 det the,barrier
R5777 T9306 T9290 conj barrier,barrier
R5778 T9307 T9308 amod basal,cell
R5779 T9308 T9306 compound cell,barrier
R578 T1039 T1031 aux has,found
R5780 T9309 T9310 punct (,e
R5781 T9310 T9306 parataxis e,barrier
R5782 T9311 T9310 dep BC,e
R5783 T9312 T9310 punct ", ",e
R5784 T9313 T9314 punct -,f
R5785 T9314 T9310 prep f,e
R5786 T9315 T9310 cc and,e
R5787 T9316 T9310 conj k,e
R5788 T9317 T9318 punct -,l
R5789 T9318 T9316 prep l,k
R579 T1040 T1031 auxpass been,found
R5790 T9319 T9310 punct ),e
R5791 T9320 T9284 punct .,varied
R5792 T9322 T9323 preconj Both,cell
R5793 T9323 T9326 nsubj cell,appeared
R5794 T9324 T9323 det the,cell
R5795 T9325 T9323 amod marginal,cell
R5796 T9327 T9328 punct (,e
R5797 T9328 T9323 parataxis e,cell
R5798 T9329 T9330 punct -,f
R5799 T9330 T9328 prep f,e
R580 T1041 T1031 prep in,found
R5800 T9331 T9328 punct ),e
R5801 T9332 T9323 cc and,cell
R5802 T9333 T9334 det the,cell
R5803 T9334 T9336 compound cell,barrier
R5804 T9335 T9334 amod basal,cell
R5805 T9336 T9323 conj barrier,cell
R5806 T9337 T9338 punct (,i
R5807 T9338 T9336 parataxis i,barrier
R5808 T9339 T9340 punct -,f
R5809 T9340 T9338 prep f,i
R581 T1042 T1043 amod several,places
R5810 T9341 T9338 punct ),i
R5811 T9342 T9343 aux to,be
R5812 T9343 T9326 xcomp be,appeared
R5813 T9344 T9343 acomp intact,be
R5814 T9345 T9326 punct .,appeared
R5815 T9347 T9348 nsubj It,was
R5816 T9349 T9348 acomp critical,was
R5817 T9350 T9348 prep for,was
R5818 T9351 T9352 det this,finding
R5819 T9352 T9350 pobj finding,for
R582 T1043 T1041 pobj places,in
R5820 T9353 T9354 mark that,block
R5821 T9354 T9348 ccomp block,was
R5822 T9355 T9354 nsubj pigmentation,block
R5823 T9356 T9354 aux did,block
R5824 T9357 T9354 neg not,block
R5825 T9358 T9359 det the,path
R5826 T9359 T9354 dobj path,block
R5827 T9360 T9359 prep of,path
R5828 T9361 T9362 det the,laser
R5829 T9362 T9360 pobj laser,of
R583 T1044 T1043 prep including,places
R5830 T9363 T9348 punct .,was
R5831 T9365 T9366 nsubj Blockage,produces
R5832 T9367 T9365 prep of,Blockage
R5833 T9368 T9369 det the,laser
R5834 T9369 T9367 pobj laser,of
R5835 T9370 T9365 prep by,Blockage
R5836 T9371 T9370 pobj pigmentation,by
R5837 T9372 T9373 det the,impression
R5838 T9373 T9366 dobj impression,produces
R5839 T9374 T9373 amod untrue,impression
R584 T1045 T1046 det the,cochlea
R5840 T9375 T9373 prep of,impression
R5841 T9376 T9377 det a,barrier
R5842 T9377 T9375 pobj barrier,of
R5843 T9378 T9377 amod discontinuous,barrier
R5844 T9379 T9380 amod marginal,cell
R5845 T9380 T9377 compound cell,barrier
R5846 T9381 T9382 punct (,c
R5847 T9382 T9377 parataxis c,barrier
R5848 T9383 T9384 punct -,d
R5849 T9384 T9382 prep d,c
R585 T1046 T1044 pobj cochlea,including
R5850 T9385 T9382 punct ),c
R5851 T9386 T9377 cc or,barrier
R5852 T9387 T9388 amod basal,cell
R5853 T9388 T9389 compound cell,barrier
R5854 T9389 T9377 conj barrier,barrier
R5855 T9390 T9391 punct (,k
R5856 T9391 T9389 parataxis k,barrier
R5857 T9392 T9393 punct -,l
R5858 T9393 T9391 prep l,k
R5859 T9394 T9391 punct ),k
R586 T1047 T1046 punct ", ",cochlea
R5860 T9395 T9366 punct .,produces
R5861 T9397 T9398 nsubjpass Comparison,aided
R5862 T9399 T9397 prep of,Comparison
R5863 T9400 T9399 pobj images,of
R5864 T9401 T9398 auxpass is,aided
R5865 T9402 T9398 prep by,aided
R5866 T9403 T9402 pcomp marking,by
R5867 T9404 T9405 det a,area
R5868 T9405 T9403 dobj area,marking
R5869 T9406 T9405 amod significant,area
R587 T1048 T1049 det the,labyrinth
R5870 T9407 T9403 prep with,marking
R5871 T9408 T9409 det a,star
R5872 T9409 T9407 pobj star,with
R5873 T9410 T9398 punct .,aided
R5874 T9412 T9413 nsubj Bars,μm
R5875 T9414 T9413 punct =,μm
R5876 T9415 T9413 nummod 10,μm
R5877 T9416 T9413 punct .,μm
R588 T1049 T1046 conj labyrinth,cochlea
R5880 T9537 T9536 prep of,Analysis
R5881 T9538 T9539 amod marginal,barriers
R5882 T9539 T9537 pobj barriers,of
R5883 T9540 T9538 cc and,marginal
R5884 T9541 T9538 conj basal,marginal
R5885 T9542 T9539 compound cell,barriers
R5886 T9543 T9536 prep in,Analysis
R5887 T9544 T9545 nmod Slc26a4,mice
R5888 T9545 T9543 pobj mice,in
R5889 T9546 T9544 punct +,Slc26a4
R589 T1050 T1049 amod vestibular,labyrinth
R5890 T9547 T9544 punct /,Slc26a4
R5891 T9548 T9544 punct +,Slc26a4
R5892 T9549 T9544 cc and,Slc26a4
R5893 T9550 T9544 conj Slc26a4,Slc26a4
R5894 T9551 T9550 punct -,Slc26a4
R5895 T9552 T9550 punct /,Slc26a4
R5896 T9553 T9550 punct -,Slc26a4
R5897 T9554 T9536 punct .,Analysis
R5898 T9556 T9557 amod Tight,junctions
R5899 T9557 T9558 nsubjpass junctions,visualized
R590 T1051 T1049 cc and,labyrinth
R5900 T9559 T9558 auxpass were,visualized
R5901 T9560 T9558 prep by,visualized
R5902 T9561 T9562 compound F,actin
R5903 T9562 T9560 pobj actin,by
R5904 T9563 T9558 prep in,visualized
R5905 T9564 T9565 amod whole,mounts
R5906 T9565 T9563 pobj mounts,in
R5907 T9566 T9565 punct -,mounts
R5908 T9567 T9565 prep of,mounts
R5909 T9568 T9569 compound stria,vascularis
R591 T1052 T1053 det the,sac
R5910 T9569 T9567 pobj vascularis,of
R5911 T9570 T9558 punct .,visualized
R5912 T9572 T9573 amod Bright,field
R5913 T9573 T9574 compound field,images
R5914 T9574 T9575 nsubjpass images,taken
R5915 T9576 T9575 auxpass were,taken
R5916 T9577 T9578 aux to,evaluate
R5917 T9578 T9575 advcl evaluate,taken
R5918 T9579 T9578 dobj pigmentation,evaluate
R5919 T9580 T9581 punct (,a
R592 T1053 T1049 conj sac,labyrinth
R5920 T9581 T9575 parataxis a,taken
R5921 T9582 T9581 punct ", ",a
R5922 T9583 T9581 conj d,a
R5923 T9584 T9583 cc and,d
R5924 T9585 T9583 conj g,d
R5925 T9586 T9581 punct ),a
R5926 T9587 T9575 punct .,taken
R5927 T9590 T9591 det the,barriers
R5928 T9591 T9589 dobj barriers,Note
R5929 T9592 T9591 amod intact,barriers
R593 T1054 T1053 amod endolymphatic,sac
R5930 T9593 T9594 amod marginal,cell
R5931 T9594 T9591 nmod cell,barriers
R5932 T9595 T9596 punct (,b
R5933 T9596 T9594 parataxis b,cell
R5934 T9597 T9596 punct ),b
R5935 T9598 T9594 cc and,cell
R5936 T9599 T9600 amod basal,cell
R5937 T9600 T9594 conj cell,cell
R5938 T9601 T9602 punct (,c
R5939 T9602 T9600 parataxis c,cell
R594 T1055 T1056 punct [,8
R5940 T9603 T9602 punct ),c
R5941 T9604 T9591 prep in,barriers
R5942 T9605 T9606 nmod Slc26a4,mice
R5943 T9606 T9604 pobj mice,in
R5944 T9607 T9605 punct +,Slc26a4
R5945 T9608 T9605 punct /,Slc26a4
R5946 T9609 T9605 punct +,Slc26a4
R5947 T9610 T9589 punct .,Note
R5948 T9612 T9613 amod Minimal,pigmentation
R5949 T9613 T9614 nsubj pigmentation,compromise
R595 T1056 T1031 parataxis 8,found
R5950 T9615 T9613 prep of,pigmentation
R5951 T9616 T9617 nmod Slc26a4,mice
R5952 T9617 T9615 pobj mice,of
R5953 T9618 T9616 punct +,Slc26a4
R5954 T9619 T9616 punct /,Slc26a4
R5955 T9620 T9616 punct +,Slc26a4
R5956 T9621 T9614 aux did,compromise
R5957 T9622 T9614 neg not,compromise
R5958 T9623 T9624 compound F,actin
R5959 T9624 T9625 compound actin,localization
R596 T1057 T1056 punct ],8
R5960 T9625 T9614 dobj localization,compromise
R5961 T9626 T9614 punct .,compromise
R5962 T9628 T9629 amod Whole,mounts
R5963 T9629 T9631 nsubjpass mounts,viewed
R5964 T9630 T9629 punct -,mounts
R5965 T9632 T9629 prep of,mounts
R5966 T9633 T9634 compound stria,vascularis
R5967 T9634 T9632 pobj vascularis,of
R5968 T9635 T9629 prep from,mounts
R5969 T9636 T9637 nmod Slc26a4,mice
R597 T1058 T1031 punct .,found
R5970 T9637 T9635 pobj mice,from
R5971 T9638 T9636 punct -,Slc26a4
R5972 T9639 T9636 punct /,Slc26a4
R5973 T9640 T9636 punct -,Slc26a4
R5974 T9641 T9631 auxpass were,viewed
R5975 T9642 T9643 preconj either,from
R5976 T9643 T9631 prep from,viewed
R5977 T9644 T9645 det the,side
R5978 T9645 T9643 pobj side,from
R5979 T9646 T9647 amod basal,cell
R598 T1060 T1061 det The,location
R5980 T9647 T9645 compound cell,side
R5981 T9648 T9649 punct (,e
R5982 T9649 T9645 parataxis e,side
R5983 T9650 T9651 punct -,f
R5984 T9651 T9649 prep f,e
R5985 T9652 T9649 punct ),e
R5986 T9653 T9643 cc or,from
R5987 T9654 T9643 conj from,from
R5988 T9655 T9656 det the,side
R5989 T9656 T9654 pobj side,from
R599 T1061 T1063 nsubjpass location,determined
R5990 T9657 T9658 amod marginal,cell
R5991 T9658 T9656 compound cell,side
R5992 T9659 T9660 punct (,h
R5993 T9660 T9656 parataxis h,side
R5994 T9661 T9662 punct -,i
R5995 T9662 T9660 prep i,h
R5996 T9663 T9660 punct ),h
R5997 T9664 T9631 punct .,viewed
R5998 T9666 T9667 amod Bright,field
R5999 T9667 T9668 compound field,images
R600 T1062 T1061 amod precise,location
R6000 T9668 T9669 nsubj images,verify
R6001 T9670 T9671 mark that,viewed
R6002 T9671 T9669 ccomp viewed,verify
R6003 T9672 T9673 det the,area
R6004 T9673 T9671 nsubjpass area,viewed
R6005 T9674 T9673 amod same,area
R6006 T9675 T9671 auxpass was,viewed
R6007 T9676 T9671 prep from,viewed
R6008 T9677 T9678 preconj either,side
R6009 T9678 T9676 pobj side,from
R601 T1064 T1061 prep of,location
R6010 T9679 T9680 punct (,d
R6011 T9680 T9669 parataxis d,verify
R6012 T9681 T9680 cc and,d
R6013 T9682 T9680 conj g,d
R6014 T9683 T9680 punct ),d
R6015 T9684 T9669 punct .,verify
R6016 T9686 T9687 nsubjpass Focus,varied
R6017 T9688 T9687 auxpass was,varied
R6018 T9689 T9690 aux to,visualize
R6019 T9690 T9687 advcl visualize,varied
R602 T1065 T1066 compound pendrin,expression
R6020 T9691 T9690 preconj either,visualize
R6021 T9692 T9693 det the,barrier
R6022 T9693 T9690 dobj barrier,visualize
R6023 T9694 T9695 amod marginal,cell
R6024 T9695 T9693 compound cell,barrier
R6025 T9696 T9697 punct (,b
R6026 T9697 T9693 parataxis b,barrier
R6027 T9698 T9697 dep SMC,b
R6028 T9699 T9697 punct ", ",b
R6029 T9700 T9697 punct ",",b
R603 T1066 T1064 pobj expression,of
R6030 T9701 T9697 conj e,b
R6031 T9702 T9701 cc and,e
R6032 T9703 T9701 conj h,e
R6033 T9704 T9697 punct ),b
R6034 T9705 T9693 cc or,barrier
R6035 T9706 T9707 det the,barrier
R6036 T9707 T9693 conj barrier,barrier
R6037 T9708 T9709 amod basal,cell
R6038 T9709 T9707 compound cell,barrier
R6039 T9710 T9711 punct (,c
R604 T1067 T1066 compound protein,expression
R6040 T9711 T9707 parataxis c,barrier
R6041 T9712 T9711 dep BC,c
R6042 T9713 T9711 punct ", ",c
R6043 T9714 T9711 punct ",",c
R6044 T9715 T9711 conj f,c
R6045 T9716 T9715 cc and,f
R6046 T9717 T9715 conj i,f
R6047 T9718 T9711 punct ),c
R6048 T9719 T9687 punct .,varied
R6049 T9721 T9722 preconj Both,cell
R605 T1068 T1063 punct ", ",determined
R6050 T9722 T9725 nsubj cell,appeared
R6051 T9723 T9722 det the,cell
R6052 T9724 T9722 amod basal,cell
R6053 T9726 T9727 punct (,f
R6054 T9727 T9722 parataxis f,cell
R6055 T9728 T9727 punct ),f
R6056 T9729 T9722 cc and,cell
R6057 T9730 T9731 det the,cell
R6058 T9731 T9733 nmod cell,barriers
R6059 T9732 T9731 amod marginal,cell
R606 T1069 T1063 advmod however,determined
R6060 T9733 T9722 conj barriers,cell
R6061 T9734 T9735 punct (,h
R6062 T9735 T9731 parataxis h,cell
R6063 T9736 T9735 punct ),h
R6064 T9737 T9738 aux to,be
R6065 T9738 T9725 xcomp be,appeared
R6066 T9739 T9738 acomp intact,be
R6067 T9740 T9725 punct .,appeared
R6068 T9742 T9743 nsubj Blockage,produces
R6069 T9744 T9742 prep of,Blockage
R607 T1070 T1063 punct ", ",determined
R6070 T9745 T9746 det the,laser
R6071 T9746 T9744 pobj laser,of
R6072 T9747 T9742 prep by,Blockage
R6073 T9748 T9747 pobj pigmentation,by
R6074 T9749 T9750 det the,impression
R6075 T9750 T9743 dobj impression,produces
R6076 T9751 T9750 amod untrue,impression
R6077 T9752 T9750 prep of,impression
R6078 T9753 T9752 punct ',of
R6079 T9754 T9752 pobj holes,of
R608 T1071 T1063 aux has,determined
R6080 T9755 T9754 punct ',holes
R6081 T9756 T9754 prep in,holes
R6082 T9757 T9758 det the,barrier
R6083 T9758 T9756 pobj barrier,in
R6084 T9759 T9760 amod marginal,cell
R6085 T9760 T9758 compound cell,barrier
R6086 T9761 T9762 punct (,e
R6087 T9762 T9758 parataxis e,barrier
R6088 T9763 T9762 punct ),e
R6089 T9764 T9758 cc or,barrier
R609 T1072 T1063 neg not,determined
R6090 T9765 T9766 amod basal,cell
R6091 T9766 T9767 compound cell,barrier
R6092 T9767 T9758 conj barrier,barrier
R6093 T9768 T9769 punct (,i
R6094 T9769 T9767 parataxis i,barrier
R6095 T9770 T9769 punct ),i
R6096 T9771 T9743 punct .,produces
R6097 T9773 T9774 nsubjpass Comparison,aided
R6098 T9775 T9773 prep of,Comparison
R6099 T9776 T9775 pobj images,of
R610 T1073 T1063 advmod yet,determined
R6100 T9777 T9774 auxpass is,aided
R6101 T9778 T9774 agent by,aided
R6102 T9779 T9778 pcomp marking,by
R6103 T9780 T9781 nummod three,areas
R6104 T9781 T9779 dobj areas,marking
R6105 T9782 T9781 amod significant,areas
R6106 T9783 T9779 prep with,marking
R6107 T9784 T9785 amod colored,stars
R6108 T9785 T9783 pobj stars,with
R6109 T9786 T9774 punct .,aided
R611 T1074 T1063 auxpass been,determined
R6110 T9788 T9789 nsubj Bars,μm
R6111 T9790 T9789 punct =,μm
R6112 T9791 T9789 nummod 10,μm
R6113 T9792 T9789 punct .,μm
R6116 T9850 T9851 compound Protein,localization
R6117 T9852 T9851 prep of,localization
R6118 T9853 T9852 pobj KCNQ1,of
R6119 T9854 T9853 punct ", ",KCNQ1
R612 T1075 T1063 punct .,determined
R6120 T9855 T9853 conj KCNE1,KCNQ1
R6121 T9856 T9855 punct ", ",KCNE1
R6122 T9857 T9855 conj SLC12A2,KCNE1
R6123 T9858 T9857 cc and,SLC12A2
R6124 T9859 T9857 conj GJB2,SLC12A2
R6125 T9860 T9851 prep in,localization
R6126 T9861 T9862 det the,wall
R6127 T9862 T9860 pobj wall,in
R6128 T9863 T9862 amod cochlear,wall
R6129 T9864 T9862 amod lateral,wall
R613 T1077 T1078 det The,variability
R6130 T9865 T9862 prep of,wall
R6131 T9866 T9867 nmod Slc26a4,mice
R6132 T9867 T9865 pobj mice,of
R6133 T9868 T9866 punct +,Slc26a4
R6134 T9869 T9866 punct /,Slc26a4
R6135 T9870 T9866 punct +,Slc26a4
R6136 T9871 T9866 cc and,Slc26a4
R6137 T9872 T9866 conj Slc26a4,Slc26a4
R6138 T9873 T9872 punct -,Slc26a4
R6139 T9874 T9872 punct /,Slc26a4
R614 T1078 T1079 nsubj variability,suggest
R6140 T9875 T9872 punct -,Slc26a4
R6141 T9876 T9851 punct .,localization
R6142 T9878 T9879 meta a,μm
R6143 T9880 T9878 punct -,a
R6144 T9881 T9878 dep f,a
R6145 T9882 T9879 punct : ,μm
R6146 T9883 T9879 dep bars,μm
R6147 T9884 T9879 punct : ,μm
R6148 T9885 T9879 nummod 10,μm
R6149 T9886 T9879 punct .,μm
R615 T1080 T1078 prep of,variability
R6150 T9889 T9888 punct ", ",SMC
R6151 T9890 T9891 amod strial,cells
R6152 T9891 T9888 appos cells,SMC
R6153 T9892 T9891 amod marginal,cells
R6154 T9893 T9888 punct ;,SMC
R6155 T9894 T9888 appos SV,SMC
R6156 T9895 T9894 punct ", ",SV
R6157 T9896 T9897 compound stria,vascularis
R6158 T9897 T9894 appos vascularis,SV
R6159 T9898 T9888 punct ;,SMC
R616 T1081 T1080 pobj deafness,of
R6160 T9899 T9888 appos BC,SMC
R6161 T9900 T9899 punct ", ",BC
R6162 T9901 T9902 amod basal,cells
R6163 T9902 T9899 appos cells,BC
R6164 T9903 T9888 punct ;,SMC
R6165 T9904 T9888 appos SL,SMC
R6166 T9905 T9904 punct ", ",SL
R6167 T9906 T9907 amod spiral,ligament
R6168 T9907 T9904 appos ligament,SL
R6169 T9908 T9888 punct .,SMC
R617 T1082 T1078 prep in,variability
R6170 T9959 T9958 prep for,Model
R6171 T9960 T9961 det the,loss
R6172 T9961 T9959 pobj loss,for
R6173 T9962 T9961 prep of,loss
R6174 T9963 T9962 pobj KCNJ10,of
R6175 T9964 T9961 prep in,loss
R6176 T9965 T9966 det the,absence
R6177 T9966 T9964 pobj absence,in
R6178 T9967 T9966 prep of,absence
R6179 T9968 T9969 compound pendrin,expression
R618 T1083 T1084 compound Pendred,syndrome
R6180 T9969 T9967 pobj expression,of
R6181 T9970 T9961 prep in,loss
R6182 T9971 T9972 compound stria,vascularis
R6183 T9972 T9970 pobj vascularis,in
R6184 T9973 T9958 punct .,Model
R6185 T9976 T9975 punct ", ",Cys
R6186 T9977 T9975 appos cysteine,Cys
R6187 T9978 T9975 punct ", ",Cys
R6188 T9979 T9975 appos Glu,Cys
R6189 T9980 T9979 punct ", ",Glu
R619 T1084 T1082 pobj syndrome,in
R6190 T9981 T9979 appos glutamate,Glu
R6191 T9982 T9975 punct ", ",Cys
R6192 T9983 T9975 appos Gly,Cys
R6193 T9984 T9983 punct ", ",Gly
R6194 T9985 T9983 appos glycine,Gly
R6195 T9986 T9975 punct ", ",Cys
R6196 T9987 T9975 appos CA,Cys
R6197 T9988 T9987 punct ", ",CA
R6198 T9989 T9990 amod carbonic,anhydrase
R6199 T9990 T9987 appos anhydrase,CA
R620 T1085 T1078 cc and,variability
R6200 T9991 T9975 punct ", ",Cys
R6201 T9992 T9975 appos GST,Cys
R6202 T9993 T9992 punct ", ",GST
R6203 T9994 T9995 compound glutathione,transferase
R6204 T9995 T9992 appos transferase,GST
R6205 T9996 T9995 punct -,transferase
R6206 T9997 T9995 compound S,transferase
R6207 T9998 T9995 punct -,transferase
R6208 T9999 T9975 punct ", ",Cys
R6209 T10000 T9975 appos GSH,Cys
R621 T1086 T1087 det the,observation
R6210 T10001 T10000 punct ", ",GSH
R6211 T10002 T10000 appos glutathione,GSH
R6212 T10003 T9975 punct .,Cys
R622 T1087 T1078 conj observation,variability
R623 T1088 T1089 mark that,is
R624 T1089 T1087 acl is,observation
R625 T1090 T1089 nsubj deafness,is
R626 T1091 T1089 advmod sometimes,is
R627 T1092 T1089 acomp late,is
R628 T1093 T1089 prep in,is
R629 T1094 T1093 pobj onset,in
R630 T1095 T1096 mark that,be
R631 T1096 T1079 ccomp be,suggest
R632 T1097 T1098 compound pendrin,dysfunction
R633 T1098 T1096 nsubj dysfunction,be
R634 T1099 T1096 aux may,be
R635 T1100 T1096 neg not,be
R636 T1101 T1102 det the,cause
R637 T1102 T1096 attr cause,be
R638 T1103 T1102 amod direct,cause
R639 T1104 T1102 prep of,cause
R640 T1105 T1104 pobj deafness,of
R641 T1106 T1079 punct .,suggest
R642 T1108 T1109 nsubj It,is
R643 T1110 T1109 acomp conceivable,is
R644 T1111 T1112 mark that,favors
R645 T1112 T1109 ccomp favors,is
R646 T1113 T1114 compound pendrin,dysfunction
R647 T1114 T1112 nsubj dysfunction,favors
R648 T1115 T1112 dobj changes,favors
R649 T1116 T1115 prep in,changes
R650 T1117 T1118 det the,levels
R651 T1118 T1116 pobj levels,in
R652 T1119 T1118 compound expression,levels
R653 T1120 T1118 prep of,levels
R654 T1121 T1120 pobj proteins,of
R655 T1122 T1123 dep that,are
R656 T1123 T1121 relcl are,proteins
R657 T1124 T1123 acomp critical,are
R658 T1125 T1124 prep for,critical
R659 T1126 T1127 det the,maintenance
R660 T1127 T1125 pobj maintenance,for
R661 T1128 T1127 prep of,maintenance
R662 T1129 T1130 det the,function
R663 T1130 T1128 pobj function,of
R664 T1131 T1130 compound hearing,function
R665 T1132 T1109 punct .,is
R666 T1134 T1135 amod Detailed,studies
R667 T1135 T1136 nsubj studies,become
R668 T1137 T1135 acl aimed,studies
R669 T1138 T1137 prep at,aimed
R670 T1139 T1138 pcomp identifying,at
R671 T1140 T1141 det the,cause
R672 T1141 T1139 dobj cause,identifying
R673 T1142 T1141 amod direct,cause
R674 T1143 T1141 prep of,cause
R675 T1144 T1143 pobj deafness,of
R676 T1145 T1141 prep in,cause
R677 T1146 T1147 compound Pendred,syndrome
R678 T1147 T1145 pobj syndrome,in
R679 T1148 T1136 aux have,become
R680 T1149 T1136 advmod recently,become
R681 T1150 T1136 acomp possible,become
R682 T1151 T1136 prep due,become
R683 T1152 T1151 pcomp to,due
R684 T1153 T1154 det the,generation
R685 T1154 T1151 pobj generation,due
R686 T1155 T1154 prep of,generation
R687 T1156 T1157 det a,antibody
R688 T1157 T1155 pobj antibody,of
R689 T1158 T1159 npadvmod pendrin,specific
R690 T1159 T1157 amod specific,antibody
R691 T1160 T1159 punct -,specific
R692 T1161 T1157 amod polyclonal,antibody
R693 T1162 T1163 punct [,9
R694 T1163 T1154 parataxis 9,generation
R695 T1164 T1163 punct ],9
R696 T1165 T1154 cc and,generation
R697 T1166 T1167 det the,development
R698 T1167 T1154 conj development,generation
R699 T1168 T1167 prep of,development
R700 T1169 T1170 nmod Slc26a4,mice
R701 T1170 T1168 pobj mice,of
R702 T1171 T1169 punct -,Slc26a4
R703 T1172 T1169 punct /,Slc26a4
R704 T1173 T1169 punct -,Slc26a4
R705 T1174 T1170 punct ", ",mice
R706 T1175 T1176 dep which,bear
R707 T1176 T1170 relcl bear,mice
R708 T1177 T1178 det a,disruption
R709 T1178 T1176 dobj disruption,bear
R710 T1179 T1178 amod targeted,disruption
R711 T1180 T1178 prep of,disruption
R712 T1181 T1182 det the,gene
R713 T1182 T1180 pobj gene,of
R714 T1183 T1182 compound mouse,gene
R715 T1184 T1182 compound Slc26a4,gene
R716 T1185 T1186 punct [,14
R717 T1186 T1136 parataxis 14,become
R718 T1187 T1186 punct ],14
R719 T1188 T1136 punct .,become
R720 T1190 T1191 det The,aim
R721 T1191 T1192 nsubj aim,was
R722 T1193 T1191 prep in,aim
R723 T1194 T1195 det the,study
R724 T1195 T1193 pobj study,in
R725 T1196 T1195 amod present,study
R726 T1197 T1198 aux to,determine
R727 T1198 T1192 xcomp determine,was
R728 T1199 T1200 det the,location
R729 T1200 T1198 dobj location,determine
R730 T1201 T1200 prep of,location
R731 T1202 T1201 pobj pendrin,of
R732 T1203 T1200 cc and,location
R733 T1204 T1205 det the,cause
R734 T1205 T1200 conj cause,location
R735 T1206 T1205 prep of,cause
R736 T1207 T1206 pobj deafness,of
R737 T1208 T1198 prep in,determine
R738 T1209 T1210 nmod Slc26a4,mice
R739 T1210 T1208 pobj mice,in
R740 T1211 T1209 punct -,Slc26a4
R741 T1212 T1209 punct /,Slc26a4
R742 T1213 T1209 punct -,Slc26a4
R743 T1214 T1192 punct .,was
R753 T1324 T1320 nmod K,concentrations
R754 T1325 T1324 punct +,K
R755 T1326 T1317 auxpass were,measured
R756 T1327 T1317 prep in,measured
R757 T1328 T1329 amod young,mice
R758 T1329 T1327 pobj mice,in
R759 T1330 T1329 amod adult,mice
R760 T1331 T1332 punct (,month
R761 T1332 T1329 parataxis month,mice
R762 T1333 T1334 quantmod 1,4
R763 T1334 T1332 nummod 4,month
R764 T1335 T1334 punct –,4
R765 T1336 T1332 prep of,month
R766 T1337 T1336 pobj age,of
R767 T1338 T1332 punct ),month
R768 T1339 T1340 dep that,have
R769 T1340 T1329 relcl have,mice
R770 T1341 T1340 preconj either,have
R771 T1342 T1343 punct (,Slc26a4
R772 T1343 T1340 parataxis Slc26a4,have
R773 T1344 T1343 punct +,Slc26a4
R774 T1345 T1343 punct /,Slc26a4
R775 T1346 T1343 punct +,Slc26a4
R776 T1347 T1343 punct ),Slc26a4
R777 T1348 T1340 cc or,have
R778 T1349 T1340 conj lack,have
R779 T1350 T1351 punct (,Slc26a4
R780 T1351 T1349 parataxis Slc26a4,lack
R781 T1352 T1351 punct -,Slc26a4
R782 T1353 T1351 punct /,Slc26a4
R783 T1354 T1351 punct -,Slc26a4
R784 T1355 T1351 punct ),Slc26a4
R785 T1356 T1357 det a,gene
R786 T1357 T1349 dobj gene,lack
R787 T1358 T1357 amod functional,gene
R788 T1359 T1357 prep for,gene
R789 T1360 T1359 pobj pendrin,for
R790 T1361 T1362 punct [,14
R791 T1362 T1349 parataxis 14,lack
R792 T1363 T1362 punct ],14
R793 T1364 T1317 punct .,measured
R794 T1366 T1367 det The,strain
R795 T1367 T1369 nsubjpass strain,chosen
R796 T1368 T1367 compound mouse,strain
R797 T1370 T1371 compound 129Sv,Ev
R798 T1371 T1367 appos Ev,strain
R799 T1372 T1371 punct /,Ev
R800 T1373 T1374 punct (,Taconic
R801 T1374 T1367 parataxis Taconic,strain
R802 T1375 T1374 punct ", ",Taconic
R803 T1376 T1374 npadvmod Germantown,Taconic
R804 T1377 T1374 punct ", ",Taconic
R805 T1378 T1374 npadvmod NY,Taconic
R806 T1379 T1374 punct ),Taconic
R807 T1380 T1369 auxpass was,chosen
R808 T1381 T1369 prep as,chosen
R809 T1382 T1383 det the,source
R810 T1383 T1381 pobj source,as
R811 T1384 T1383 prep of,source
R812 T1385 T1386 nmod Slc26a4,controls
R813 T1386 T1384 pobj controls,of
R814 T1387 T1385 punct +,Slc26a4
R815 T1388 T1385 punct /,Slc26a4
R816 T1389 T1385 punct +,Slc26a4
R817 T1390 T1369 punct ", ",chosen
R818 T1391 T1392 mark since,propagated
R819 T1392 T1369 advcl propagated,chosen
R820 T1393 T1394 nmod Slc26a4,mice
R821 T1394 T1392 nsubjpass mice,propagated
R822 T1395 T1393 punct -,Slc26a4
R823 T1396 T1393 punct /,Slc26a4
R824 T1397 T1393 punct -,Slc26a4
R825 T1398 T1392 auxpass were,propagated
R826 T1399 T1392 prep in,propagated
R827 T1400 T1401 nmod the,strain
R828 T1401 T1399 pobj strain,in
R829 T1402 T1401 det this,strain
R830 T1403 T1392 cc and,propagated
R831 T1404 T1392 conj generated,propagated
R832 T1405 T1404 advcl using,generated
R833 T1406 T1407 det a,line
R834 T1407 T1405 dobj line,using
R835 T1408 T1409 compound stem,cell
R836 T1409 T1407 compound cell,line
R837 T1410 T1407 acl derived,line
R838 T1411 T1410 prep from,derived
R839 T1412 T1413 compound 129Sv,Ev
R840 T1413 T1411 pobj Ev,from
R841 T1414 T1413 punct /,Ev
R842 T1415 T1369 punct .,chosen
R843 T1417 T1418 nsubj Slc26a4,were
R844 T1419 T1417 punct +,Slc26a4
R845 T1420 T1417 punct /,Slc26a4
R846 T1421 T1417 punct +,Slc26a4
R847 T1422 T1417 cc and,Slc26a4
R848 T1423 T1417 conj Slc26a4,Slc26a4
R849 T1424 T1423 punct -,Slc26a4
R850 T1425 T1423 punct /,Slc26a4
R851 T1426 T1423 punct -,Slc26a4
R852 T1427 T1418 attr agouti,were
R853 T1428 T1418 punct .,were
R854 T1430 T1431 nsubj They,differ
R855 T1432 T1431 aux did,differ
R856 T1433 T1431 neg not,differ
R857 T1434 T1431 prep in,differ
R858 T1435 T1436 compound coat,color
R859 T1436 T1434 pobj color,in
R860 T1437 T1431 punct .,differ
R861 T1439 T1440 nsubjpass Differences,verified
R862 T1441 T1439 prep in,Differences
R863 T1442 T1441 pobj pigmentation,in
R864 T1443 T1440 auxpass were,verified
R865 T1444 T1440 advcl using,verified
R866 T1445 T1446 amod coisogenic,Slc26a4
R867 T1446 T1444 dobj Slc26a4,using
R868 T1447 T1448 npadvmod age,matched
R869 T1448 T1446 amod matched,Slc26a4
R870 T1449 T1448 punct -,matched
R871 T1450 T1446 punct +,Slc26a4
R872 T1451 T1446 punct /,Slc26a4
R873 T1452 T1446 punct +,Slc26a4
R874 T1453 T1446 cc and,Slc26a4
R875 T1454 T1446 conj Slc26a4,Slc26a4
R876 T1455 T1454 punct -,Slc26a4
R877 T1456 T1454 punct /,Slc26a4
R878 T1457 T1454 punct -,Slc26a4
R879 T1458 T1459 dep that,bred
R880 T1459 T1446 relcl bred,Slc26a4
R881 T1460 T1459 auxpass were,bred
R882 T1461 T1459 prep in,bred
R883 T1462 T1461 pobj parallel,in
R884 T1463 T1440 punct .,verified
R885 T1465 T1466 nsubjpass Expression,studied
R886 T1467 T1465 prep of,Expression
R887 T1468 T1469 amod key,proteins
R888 T1469 T1467 pobj proteins,of
R889 T1470 T1469 acl involved,proteins
R890 T1471 T1470 prep in,involved
R891 T1472 T1473 det the,generation
R892 T1473 T1471 pobj generation,in
R893 T1474 T1473 prep of,generation
R894 T1475 T1476 det the,potential
R895 T1476 T1474 pobj potential,of
R896 T1477 T1476 amod endocochlear,potential
R897 T1478 T1473 cc and,generation
R898 T1479 T1480 det the,transport
R899 T1480 T1473 conj transport,generation
R900 T1481 T1480 prep of,transport
R901 T1482 T1481 pobj K,of
R902 T1483 T1482 punct +,K
R903 T1484 T1466 auxpass was,studied
R904 T1485 T1466 advcl using,studied
R905 T1486 T1487 amod confocal,immunocytochemistry
R906 T1487 T1485 dobj immunocytochemistry,using
R907 T1488 T1487 cc and,immunocytochemistry
R908 T1489 T1490 amod quantitative,PCR
R909 T1490 T1487 conj PCR,immunocytochemistry
R910 T1491 T1490 compound RT,PCR
R911 T1492 T1490 punct -,PCR
R912 T1493 T1466 punct .,studied
R913 T1495 T1496 nmod K,secretion
R914 T1496 T1498 nsubjpass secretion,measured
R915 T1497 T1495 punct +,K
R916 T1499 T1496 cc and,secretion
R917 T1500 T1501 det the,generation
R918 T1501 T1496 conj generation,secretion
R919 T1502 T1501 prep of,generation
R920 T1503 T1504 det the,potential
R921 T1504 T1502 pobj potential,of
R922 T1505 T1504 amod endocochlear,potential
R923 T1506 T1498 auxpass were,measured
R924 T1507 T1498 advcl using,measured
R925 T1508 T1509 amod electrophysiological,techniques
R926 T1509 T1507 dobj techniques,using
R927 T1510 T1498 punct .,measured
R928 T1512 T1513 det All,experiments
R929 T1513 T1514 nsubjpass experiments,approved
R930 T1515 T1514 auxpass were,approved
R931 T1516 T1514 agent by,approved
R932 T1517 T1518 det the,Committee
R933 T1518 T1516 pobj Committee,by
R934 T1519 T1520 nmod Institutional,Animal
R935 T1520 T1518 nmod Animal,Committee
R936 T1521 T1520 appos Care,Animal
R937 T1522 T1521 cc and,Care
R938 T1523 T1521 conj Use,Care
R939 T1524 T1518 prep of,Committee
R940 T1525 T1526 compound Kansas,University
R941 T1526 T1524 pobj University,of
R942 T1527 T1526 compound State,University
R943 T1528 T1514 punct .,approved
R944 T1678 T1679 amod Confocal,immunocytochemistry
R945 T1681 T1682 nsubjpass Animals,anesthetized
R946 T1683 T1682 auxpass were,anesthetized
R947 T1684 T1682 advmod deeply,anesthetized
R948 T1685 T1682 prep with,anesthetized
R949 T1686 T1687 compound sodium,pentobarbital
R950 T1687 T1685 pobj pentobarbital,with
R951 T1688 T1689 punct (,mg
R952 T1689 T1687 parataxis mg,pentobarbital
R953 T1690 T1689 nummod 100,mg
R954 T1691 T1692 punct /,kg
R955 T1692 T1689 prep kg,mg
R956 T1693 T1689 advmod i.p.,mg
R957 T1694 T1689 punct ),mg
R958 T1695 T1682 cc and,anesthetized
R959 T1696 T1697 advmod transcardially,perfused
R960 T1697 T1682 conj perfused,anesthetized
R961 T1698 T1697 prep with,perfused
R962 T1699 T1700 det a,solution
R963 T1700 T1698 pobj solution,with
R964 T1701 T1702 npadvmod Cl,free
R965 T1702 T1700 amod free,solution
R966 T1703 T1701 punct -,Cl
R967 T1704 T1702 punct -,free
R968 T1705 T1706 npadvmod phosphate,buffered
R969 T1706 T1700 amod buffered,solution
R970 T1707 T1706 punct -,buffered
R971 T1708 T1709 nmod Na,gluconate
R972 T1709 T1700 compound gluconate,solution
R973 T1710 T1708 punct -,Na
R974 T1711 T1700 acl containing,solution
R975 T1712 T1713 nummod 4,%
R976 T1713 T1714 compound %,paraformaldehyde
R977 T1714 T1711 dobj paraformaldehyde,containing
R978 T1715 T1682 punct .,anesthetized
R979 T1717 T1718 amod Temporal,bones
R980 T1718 T1719 nsubjpass bones,removed
R981 T1720 T1719 auxpass were,removed
R982 T1721 T1719 cc and,removed
R983 T1722 T1723 nsubj cochleae,fixed
R984 T1723 T1719 conj fixed,removed
R985 T1724 T1723 prep by,fixed
R986 T1725 T1726 amod perilymphatic,perfusion
R987 T1726 T1724 pobj perfusion,by
R988 T1727 T1723 punct ", ",fixed
R989 T1728 T1723 conj decalcified,fixed
R990 T1729 T1728 prep in,decalcified
R991 T1730 T1729 pobj EDTA,in
R992 T1731 T1728 punct ", ",decalcified
R993 T1732 T1728 conj processed,decalcified
R994 T1733 T1732 prep through,processed
R995 T1734 T1735 det a,gradient
R996 T1735 T1733 pobj gradient,through
R997 T1736 T1735 compound sucrose,gradient
R998 T1737 T1732 cc and,processed
R999 T1738 T1732 conj infiltrated,processed