PMC:516044 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T234	0-4	NN	denotes	Loss
T236	0-118	sentence	denotes	Loss of KCNJ10 protein expression abolishes endocochlear potential and causes deafness in Pendred syndrome mouse model
T237	5-7	IN	denotes	of
T238	8-14	NN	denotes	KCNJ10
T240	15-22	NN	denotes	protein
T239	23-33	NN	denotes	expression
T235	34-43	VBZ	denotes	abolishes
T241	44-56	JJ	denotes	endocochlear
T242	57-66	NN	denotes	potential
T243	67-70	CC	denotes	and
T244	71-77	VBZ	denotes	causes
T245	78-86	NN	denotes	deafness
T246	87-89	IN	denotes	in
T247	90-97	NNP	denotes	Pendred
T248	98-106	NN	denotes	syndrome
T250	107-112	NN	denotes	mouse
T249	113-118	NN	denotes	model
T251	118-119	sentence	denotes
T253	129-139	NN	denotes	Background
T252	129-139	sentence	denotes	Background
T254	139-304	sentence	denotes	Pendred syndrome, a common autosomal-recessive disorder characterized by congenital deafness and goiter, is caused by mutations of SLC26A4, which codes for pendrin.
T255	140-147	NNP	denotes	Pendred
T256	148-156	NN	denotes	syndrome
T258	156-158	,	denotes	,
T259	158-159	DT	denotes	a
T261	160-166	JJ	denotes	common
T262	167-176	JJ	denotes	autosomal
T264	176-177	HYPH	denotes	-
T263	177-186	JJ	denotes	recessive
T260	187-195	NN	denotes	disorder
T265	196-209	VBN	denotes	characterized
T266	210-212	IN	denotes	by
T267	213-223	JJ	denotes	congenital
T268	224-232	NN	denotes	deafness
T269	233-236	CC	denotes	and
T270	237-243	NN	denotes	goiter
T271	243-245	,	denotes	,
T272	245-247	VBZ	denotes	is
T257	248-254	VBN	denotes	caused
T273	255-257	IN	denotes	by
T274	258-267	NNS	denotes	mutations
T275	268-270	IN	denotes	of
T276	271-278	NN	denotes	SLC26A4
T277	278-280	,	denotes	,
T278	280-285	WDT	denotes	which
T279	286-291	VBZ	denotes	codes
T280	292-295	IN	denotes	for
T281	296-303	NN	denotes	pendrin
T282	303-304	.	denotes	.
T283	304-446	sentence	denotes	We investigated the relationship between pendrin and deafness using mice that have (Slc26a4+/+) or lack a complete Slc26a4 gene (Slc26a4-/-).
T284	305-307	PRP	denotes	We
T285	308-320	VBD	denotes	investigated
T286	321-324	DT	denotes	the
T287	325-337	NN	denotes	relationship
T288	338-345	IN	denotes	between
T289	346-353	NN	denotes	pendrin
T290	354-357	CC	denotes	and
T291	358-366	NN	denotes	deafness
T292	367-372	VBG	denotes	using
T293	373-377	NNS	denotes	mice
T294	378-382	WDT	denotes	that
T295	383-387	VBP	denotes	have
T296	388-389	-LRB-	denotes	(
T297	389-396	NN	denotes	Slc26a4
T298	396-397	SYM	denotes	+
T299	397-398	HYPH	denotes	/
T300	398-399	SYM	denotes	+
T301	399-400	-RRB-	denotes	)
T302	401-403	CC	denotes	or
T303	404-408	VBP	denotes	lack
T304	409-410	DT	denotes	a
T306	411-419	JJ	denotes	complete
T307	420-427	NN	denotes	Slc26a4
T305	428-432	NN	denotes	gene
T308	433-434	-LRB-	denotes	(
T310	434-441	NN	denotes	Slc26a4
T311	441-442	SYM	denotes	-
T312	442-443	HYPH	denotes	/
T309	443-444	SYM	denotes	-
T313	444-445	-RRB-	denotes	)
T314	445-446	.	denotes	.
T315	446-455	sentence	denotes	Methods
T316	448-455	NNS	denotes	Methods
T317	455-544	sentence	denotes	Expression of pendrin and other proteins was determined by confocal immunocytochemistry.
T318	456-466	NN	denotes	Expression
T320	467-469	IN	denotes	of
T321	470-477	NN	denotes	pendrin
T322	478-481	CC	denotes	and
T323	482-487	JJ	denotes	other
T324	488-496	NN	denotes	proteins
T325	497-500	VBD	denotes	was
T319	501-511	VBN	denotes	determined
T326	512-514	IN	denotes	by
T327	515-523	JJ	denotes	confocal
T328	524-543	NN	denotes	immunocytochemistry
T329	543-544	.	denotes	.
T330	544-602	sentence	denotes	Expression of mRNA was determined by quantitative RT-PCR.
T331	545-555	NN	denotes	Expression
T333	556-558	IN	denotes	of
T334	559-563	NN	denotes	mRNA
T335	564-567	VBD	denotes	was
T332	568-578	VBN	denotes	determined
T336	579-581	IN	denotes	by
T337	582-594	JJ	denotes	quantitative
T339	595-597	NN	denotes	RT
T340	597-598	HYPH	denotes	-
T338	598-601	NN	denotes	PCR
T341	601-602	.	denotes	.
T342	602-720	sentence	denotes	The endocochlear potential and the endolymphatic K+ concentration were measured with double-barreled microelectrodes.
T343	603-606	DT	denotes	The
T1314	603-606	DT	denotes	The
T345	607-619	JJ	denotes	endocochlear
T1316	607-619	JJ	denotes	endocochlear
T344	620-629	NN	denotes	potential
T1315	620-629	NN	denotes	potential
T347	630-633	CC	denotes	and
T1318	630-633	CC	denotes	and
T348	634-637	DT	denotes	the
T1319	634-637	DT	denotes	the
T350	638-651	JJ	denotes	endolymphatic
T1321	638-651	JJ	denotes	endolymphatic
T351	652-653	NN	denotes	K
T352	653-654	SYM	denotes	+
T349	655-668	NN	denotes	concentration
T353	669-673	VBD	denotes	were
T346	674-682	VBN	denotes	measured
T354	683-687	IN	denotes	with
T355	688-694	JJ	denotes	double
T357	694-695	HYPH	denotes	-
T356	695-703	JJ	denotes	barreled
T358	704-719	NNS	denotes	microelectrodes
T359	719-720	.	denotes	.
T360	720-805	sentence	denotes	Currents generated by the stria marginal cells were recorded with a vibrating probe.
T361	721-729	NNS	denotes	Currents
T363	730-739	VBN	denotes	generated
T364	740-742	IN	denotes	by
T365	743-746	DT	denotes	the
T367	747-752	NN	denotes	stria
T368	753-761	JJ	denotes	marginal
T366	762-767	NNS	denotes	cells
T369	768-772	VBD	denotes	were
T362	773-781	VBN	denotes	recorded
T370	782-786	IN	denotes	with
T371	787-788	DT	denotes	a
T373	789-798	VBG	denotes	vibrating
T372	799-804	NN	denotes	probe
T374	804-805	.	denotes	.
T375	805-921	sentence	denotes	Tissue masses were evaluated by morphometric distance measurements and pigmentation was quantified by densitometry.
T376	806-812	NN	denotes	Tissue
T377	813-819	NNS	denotes	masses
T379	820-824	VBD	denotes	were
T378	825-834	VBN	denotes	evaluated
T380	835-837	IN	denotes	by
T381	838-850	JJ	denotes	morphometric
T383	851-859	NN	denotes	distance
T382	860-872	NNS	denotes	measurements
T384	873-876	CC	denotes	and
T385	877-889	NN	denotes	pigmentation
T387	890-893	VBD	denotes	was
T386	894-904	VBN	denotes	quantified
T388	905-907	IN	denotes	by
T389	908-920	NN	denotes	densitometry
T390	920-921	.	denotes	.
T391	921-930	sentence	denotes	Results
T392	923-930	NNS	denotes	Results
T393	930-1088	sentence	denotes	Pendrin was found in the cochlea in apical membranes of spiral prominence cells and spindle-shaped cells of stria vascularis, in outer sulcus and root cells.
T394	931-938	NN	denotes	Pendrin
T396	939-942	VBD	denotes	was
T395	943-948	VBN	denotes	found
T397	949-951	IN	denotes	in
T398	952-955	DT	denotes	the
T399	956-963	NN	denotes	cochlea
T400	964-966	IN	denotes	in
T401	967-973	JJ	denotes	apical
T402	974-983	NNS	denotes	membranes
T403	984-986	IN	denotes	of
T404	987-993	JJ	denotes	spiral
T406	994-1004	NN	denotes	prominence
T405	1005-1010	NNS	denotes	cells
T407	1011-1014	CC	denotes	and
T408	1015-1022	NN	denotes	spindle
T410	1022-1023	HYPH	denotes	-
T409	1023-1029	VBN	denotes	shaped
T411	1030-1035	NNS	denotes	cells
T412	1036-1038	IN	denotes	of
T413	1039-1044	NN	denotes	stria
T414	1045-1055	NN	denotes	vascularis
T415	1055-1057	,	denotes	,
T416	1057-1059	IN	denotes	in
T417	1060-1065	JJ	denotes	outer
T419	1066-1072	NN	denotes	sulcus
T420	1073-1076	CC	denotes	and
T421	1077-1081	NN	denotes	root
T418	1082-1087	NNS	denotes	cells
T422	1087-1088	.	denotes	.
T423	1088-1225	sentence	denotes	Endolymph volume in Slc26a4-/- mice was increased and tissue masses in areas normally occupied by type I and II fibrocytes were reduced.
T424	1089-1098	NN	denotes	Endolymph
T425	1099-1105	NN	denotes	volume
T427	1106-1108	IN	denotes	in
T428	1109-1116	NN	denotes	Slc26a4
T430	1116-1117	SYM	denotes	-
T431	1117-1118	HYPH	denotes	/
T432	1118-1119	SYM	denotes	-
T429	1120-1124	NNS	denotes	mice
T433	1125-1128	VBD	denotes	was
T426	1129-1138	VBN	denotes	increased
T434	1139-1142	CC	denotes	and
T435	1143-1149	NN	denotes	tissue
T436	1150-1156	NNS	denotes	masses
T438	1157-1159	IN	denotes	in
T439	1160-1165	NNS	denotes	areas
T440	1166-1174	RB	denotes	normally
T441	1175-1183	VBN	denotes	occupied
T442	1184-1186	IN	denotes	by
T443	1187-1191	NN	denotes	type
T444	1192-1193	CD	denotes	I
T446	1194-1197	CC	denotes	and
T447	1198-1200	CD	denotes	II
T445	1201-1211	NNS	denotes	fibrocytes
T448	1212-1216	VBD	denotes	were
T437	1217-1224	VBN	denotes	reduced
T449	1224-1225	.	denotes	.
T450	1225-1383	sentence	denotes	Slc26a4-/- mice lacked the endocochlear potential, which is generated across the basal cell barrier by the K+ channel KCNJ10 localized in intermediate cells.
T451	1226-1233	NN	denotes	Slc26a4
T453	1233-1234	SYM	denotes	-
T454	1234-1235	HYPH	denotes	/
T455	1235-1236	SYM	denotes	-
T452	1237-1241	NNS	denotes	mice
T456	1242-1248	VBD	denotes	lacked
T457	1249-1252	DT	denotes	the
T459	1253-1265	JJ	denotes	endocochlear
T458	1266-1275	NN	denotes	potential
T460	1275-1277	,	denotes	,
T461	1277-1282	WDT	denotes	which
T463	1283-1285	VBZ	denotes	is
T462	1286-1295	VBN	denotes	generated
T464	1296-1302	IN	denotes	across
T465	1303-1306	DT	denotes	the
T467	1307-1312	JJ	denotes	basal
T468	1313-1317	NN	denotes	cell
T466	1318-1325	NN	denotes	barrier
T469	1326-1328	IN	denotes	by
T470	1329-1332	DT	denotes	the
T472	1333-1334	NN	denotes	K
T473	1334-1335	SYM	denotes	+
T471	1336-1343	NN	denotes	channel
T474	1344-1350	NN	denotes	KCNJ10
T475	1351-1360	VBN	denotes	localized
T476	1361-1363	IN	denotes	in
T477	1364-1376	JJ	denotes	intermediate
T478	1377-1382	NNS	denotes	cells
T479	1382-1383	.	denotes	.
T480	1383-1465	sentence	denotes	Stria vascularis was hyperpigmented, suggesting unalleviated free radical damage.
T481	1384-1389	NN	denotes	Stria
T482	1390-1400	NN	denotes	vascularis
T483	1401-1404	VBD	denotes	was
T484	1405-1419	JJ	denotes	hyperpigmented
T485	1419-1421	,	denotes	,
T486	1421-1431	VBG	denotes	suggesting
T487	1432-1444	JJ	denotes	unalleviated
T489	1445-1449	JJ	denotes	free
T490	1450-1457	NN	denotes	radical
T488	1458-1464	NN	denotes	damage
T491	1464-1465	.	denotes	.
T492	1465-1584	sentence	denotes	The basal cell barrier appeared intact; intermediate cells and KCNJ10 mRNA were present but KCNJ10 protein was absent.
T493	1466-1469	DT	denotes	The
T495	1470-1475	JJ	denotes	basal
T496	1476-1480	NN	denotes	cell
T494	1481-1488	NN	denotes	barrier
T497	1489-1497	VBD	denotes	appeared
T499	1498-1504	JJ	denotes	intact
T500	1504-1505	:	denotes	;
T501	1506-1518	JJ	denotes	intermediate
T502	1519-1524	NNS	denotes	cells
T503	1525-1528	CC	denotes	and
T504	1529-1535	NN	denotes	KCNJ10
T505	1536-1540	NN	denotes	mRNA
T498	1541-1545	VBD	denotes	were
T506	1546-1553	JJ	denotes	present
T507	1554-1557	CC	denotes	but
T508	1558-1564	NN	denotes	KCNJ10
T509	1565-1572	NN	denotes	protein
T510	1573-1576	VBD	denotes	was
T511	1577-1583	JJ	denotes	absent
T512	1583-1584	.	denotes	.
T513	1584-1794	sentence	denotes	Endolymphatic K+ concentrations were normal and membrane proteins necessary for K+ secretion were present, including the K+ channel KCNQ1 and KCNE1, Na+/2Cl-/K+ cotransporter SLC12A2 and the gap junction GJB2.
T514	1585-1598	JJ	denotes	Endolymphatic
T516	1599-1600	NN	denotes	K
T517	1600-1601	SYM	denotes	+
T515	1602-1616	NNS	denotes	concentrations
T518	1617-1621	VBD	denotes	were
T519	1622-1628	JJ	denotes	normal
T520	1629-1632	CC	denotes	and
T521	1633-1641	NN	denotes	membrane
T522	1642-1650	NN	denotes	proteins
T524	1651-1660	JJ	denotes	necessary
T525	1661-1664	IN	denotes	for
T526	1665-1666	NN	denotes	K
T528	1666-1667	SYM	denotes	+
T527	1668-1677	NN	denotes	secretion
T523	1678-1682	VBD	denotes	were
T529	1683-1690	JJ	denotes	present
T530	1690-1692	,	denotes	,
T531	1692-1701	VBG	denotes	including
T532	1702-1705	DT	denotes	the
T534	1706-1707	NN	denotes	K
T535	1707-1708	SYM	denotes	+
T533	1709-1716	NN	denotes	channel
T536	1717-1722	NN	denotes	KCNQ1
T537	1723-1726	CC	denotes	and
T538	1727-1732	NN	denotes	KCNE1
T539	1732-1734	,	denotes	,
T540	1734-1736	NN	denotes	Na
T542	1736-1737	SYM	denotes	+
T543	1737-1738	HYPH	denotes	/
T544	1738-1741	NN	denotes	2Cl
T545	1741-1742	SYM	denotes	-
T546	1742-1743	HYPH	denotes	/
T547	1743-1744	NN	denotes	K
T548	1744-1745	SYM	denotes	+
T541	1746-1759	NN	denotes	cotransporter
T549	1760-1767	NN	denotes	SLC12A2
T550	1768-1771	CC	denotes	and
T551	1772-1775	DT	denotes	the
T553	1776-1779	NN	denotes	gap
T552	1780-1788	NN	denotes	junction
T554	1789-1793	NN	denotes	GJB2
T555	1793-1794	.	denotes	.
T556	1794-1807	sentence	denotes	Conclusions
T557	1796-1807	NNS	denotes	Conclusions
T558	1807-2013	sentence	denotes	These observations demonstrate that pendrin dysfunction leads to a loss of KCNJ10 protein expression and a loss of the endocochlear potential, which may be the direct cause of deafness in Pendred syndrome.
T559	1808-1813	DT	denotes	These
T560	1814-1826	NNS	denotes	observations
T561	1827-1838	VBP	denotes	demonstrate
T562	1839-1843	IN	denotes	that
T564	1844-1851	NN	denotes	pendrin
T565	1852-1863	NN	denotes	dysfunction
T563	1864-1869	VBZ	denotes	leads
T566	1870-1872	IN	denotes	to
T567	1873-1874	DT	denotes	a
T568	1875-1879	NN	denotes	loss
T569	1880-1882	IN	denotes	of
T570	1883-1889	NN	denotes	KCNJ10
T572	1890-1897	NN	denotes	protein
T571	1898-1908	NN	denotes	expression
T573	1909-1912	CC	denotes	and
T574	1913-1914	DT	denotes	a
T575	1915-1919	NN	denotes	loss
T576	1920-1922	IN	denotes	of
T577	1923-1926	DT	denotes	the
T579	1927-1939	JJ	denotes	endocochlear
T578	1940-1949	NN	denotes	potential
T580	1949-1951	,	denotes	,
T581	1951-1956	WDT	denotes	which
T583	1957-1960	MD	denotes	may
T582	1961-1963	VB	denotes	be
T584	1964-1967	DT	denotes	the
T586	1968-1974	JJ	denotes	direct
T585	1975-1980	NN	denotes	cause
T587	1981-1983	IN	denotes	of
T588	1984-1992	NN	denotes	deafness
T589	1993-1995	IN	denotes	in
T590	1996-2003	NNP	denotes	Pendred
T591	2004-2012	NN	denotes	syndrome
T592	2012-2013	.	denotes	.
T770	2026-2033	NNP	denotes	Pendred
T771	2034-2042	NN	denotes	syndrome
T772	2043-2045	VBZ	denotes	is
T773	2046-2047	DT	denotes	a
T775	2048-2058	RB	denotes	relatively
T776	2059-2065	JJ	denotes	common
T777	2066-2075	JJ	denotes	autosomal
T779	2075-2076	HYPH	denotes	-
T778	2076-2085	JJ	denotes	recessive
T774	2086-2094	NN	denotes	disorder
T780	2095-2108	VBN	denotes	characterized
T781	2109-2111	IN	denotes	by
T782	2112-2120	NN	denotes	deafness
T783	2121-2124	CC	denotes	and
T784	2125-2131	NN	denotes	goiter
T785	2132-2133	-LRB-	denotes	[
T786	2133-2134	CD	denotes	1
T787	2134-2135	-RRB-	denotes	]
T788	2135-2136	.	denotes	.
T789	2136-2238	sentence	denotes	The syndrome is caused by mutations of the PDS gene SLC26A4, which codes for the protein pendrin [2].
T790	2137-2140	DT	denotes	The
T791	2141-2149	NN	denotes	syndrome
T793	2150-2152	VBZ	denotes	is
T792	2153-2159	VBN	denotes	caused
T794	2160-2162	IN	denotes	by
T795	2163-2172	NNS	denotes	mutations
T796	2173-2175	IN	denotes	of
T797	2176-2179	DT	denotes	the
T799	2180-2183	NN	denotes	PDS
T798	2184-2188	NN	denotes	gene
T800	2189-2196	NN	denotes	SLC26A4
T801	2196-2198	,	denotes	,
T802	2198-2203	WDT	denotes	which
T803	2204-2209	VBZ	denotes	codes
T804	2210-2213	IN	denotes	for
T805	2214-2217	DT	denotes	the
T807	2218-2225	NN	denotes	protein
T806	2226-2233	NN	denotes	pendrin
T808	2234-2235	-LRB-	denotes	[
T809	2235-2236	CD	denotes	2
T810	2236-2237	-RRB-	denotes	]
T811	2237-2238	.	denotes	.
T812	2238-2352	sentence	denotes	Deafness is congenital and generally profound although sometimes late in onset and provoked by light head injury.
T813	2239-2247	NN	denotes	Deafness
T814	2248-2250	VBZ	denotes	is
T815	2251-2261	JJ	denotes	congenital
T816	2262-2265	CC	denotes	and
T817	2266-2275	RB	denotes	generally
T818	2276-2284	JJ	denotes	profound
T819	2285-2293	IN	denotes	although
T821	2294-2303	RB	denotes	sometimes
T820	2304-2308	JJ	denotes	late
T822	2309-2311	IN	denotes	in
T823	2312-2317	NN	denotes	onset
T824	2318-2321	CC	denotes	and
T825	2322-2330	VBN	denotes	provoked
T826	2331-2333	IN	denotes	by
T827	2334-2339	JJ	denotes	light
T829	2340-2344	NN	denotes	head
T828	2345-2351	NN	denotes	injury
T830	2351-2352	.	denotes	.
T831	2352-2388	sentence	denotes	Vestibular dysfunction is uncommon.
T832	2353-2363	JJ	denotes	Vestibular
T833	2364-2375	NN	denotes	dysfunction
T834	2376-2378	VBZ	denotes	is
T835	2379-2387	JJ	denotes	uncommon
T836	2387-2388	.	denotes	.
T837	2388-2450	sentence	denotes	Goiter is variable and generally develops around puberty [3].
T838	2389-2395	NN	denotes	Goiter
T839	2396-2398	VBZ	denotes	is
T840	2399-2407	JJ	denotes	variable
T841	2408-2411	CC	denotes	and
T842	2412-2421	RB	denotes	generally
T843	2422-2430	VBZ	denotes	develops
T844	2431-2437	IN	denotes	around
T845	2438-2445	NN	denotes	puberty
T846	2446-2447	-LRB-	denotes	[
T847	2447-2448	CD	denotes	3
T848	2448-2449	-RRB-	denotes	]
T849	2449-2450	.	denotes	.
T850	2450-2649	sentence	denotes	The cause of goiter appears to be an impairment of iodide fixation in the follicular lumen due to a reduced rate of iodide transport across the apical membrane of thyroid gland epithelial cells [4].
T851	2451-2454	DT	denotes	The
T852	2455-2460	NN	denotes	cause
T854	2461-2463	IN	denotes	of
T855	2464-2470	NN	denotes	goiter
T853	2471-2478	VBZ	denotes	appears
T856	2479-2481	TO	denotes	to
T857	2482-2484	VB	denotes	be
T858	2485-2487	DT	denotes	an
T859	2488-2498	NN	denotes	impairment
T860	2499-2501	IN	denotes	of
T861	2502-2508	NN	denotes	iodide
T862	2509-2517	NN	denotes	fixation
T863	2518-2520	IN	denotes	in
T864	2521-2524	DT	denotes	the
T866	2525-2535	JJ	denotes	follicular
T865	2536-2541	NN	denotes	lumen
T867	2542-2545	IN	denotes	due
T868	2546-2548	IN	denotes	to
T869	2549-2550	DT	denotes	a
T871	2551-2558	VBN	denotes	reduced
T870	2559-2563	NN	denotes	rate
T872	2564-2566	IN	denotes	of
T873	2567-2573	NN	denotes	iodide
T874	2574-2583	NN	denotes	transport
T875	2584-2590	IN	denotes	across
T876	2591-2594	DT	denotes	the
T878	2595-2601	JJ	denotes	apical
T877	2602-2610	NN	denotes	membrane
T879	2611-2613	IN	denotes	of
T880	2614-2621	JJ	denotes	thyroid
T881	2622-2627	NN	denotes	gland
T883	2628-2638	JJ	denotes	epithelial
T882	2639-2644	NNS	denotes	cells
T884	2645-2646	-LRB-	denotes	[
T885	2646-2647	CD	denotes	4
T886	2647-2648	-RRB-	denotes	]
T887	2648-2649	.	denotes	.
T888	2649-2794	sentence	denotes	A positive perchlorate discharge test and an enlarged vestibular aqueduct appear to be the most reliable clinical signs of Pendred syndrome [5].
T889	2650-2651	DT	denotes	A
T891	2652-2660	JJ	denotes	positive
T892	2661-2672	NN	denotes	perchlorate
T893	2673-2682	NN	denotes	discharge
T890	2683-2687	NN	denotes	test
T895	2688-2691	CC	denotes	and
T896	2692-2694	DT	denotes	an
T898	2695-2703	VBN	denotes	enlarged
T899	2704-2714	JJ	denotes	vestibular
T897	2715-2723	NN	denotes	aqueduct
T894	2724-2730	VBP	denotes	appear
T900	2731-2733	TO	denotes	to
T901	2734-2736	VB	denotes	be
T902	2737-2740	DT	denotes	the
T904	2741-2745	RBS	denotes	most
T905	2746-2754	JJ	denotes	reliable
T906	2755-2763	JJ	denotes	clinical
T903	2764-2769	NNS	denotes	signs
T907	2770-2772	IN	denotes	of
T908	2773-2780	NNP	denotes	Pendred
T909	2781-2789	NN	denotes	syndrome
T910	2790-2791	-LRB-	denotes	[
T911	2791-2792	CD	denotes	5
T912	2792-2793	-RRB-	denotes	]
T913	2793-2794	.	denotes	.
T914	2794-2878	sentence	denotes	Pendrin is an anion exchanger that can transport Cl-, I-, HCO3 - and formate [6,7].
T915	2795-2802	NN	denotes	Pendrin
T916	2803-2805	VBZ	denotes	is
T917	2806-2808	DT	denotes	an
T919	2809-2814	NN	denotes	anion
T918	2815-2824	NN	denotes	exchanger
T920	2825-2829	WDT	denotes	that
T922	2830-2833	MD	denotes	can
T921	2834-2843	VB	denotes	transport
T923	2844-2846	NN	denotes	Cl
T924	2846-2847	SYM	denotes	-
T925	2847-2849	,	denotes	,
T926	2849-2850	NN	denotes	I
T927	2850-2851	SYM	denotes	-
T928	2851-2853	,	denotes	,
T929	2853-2857	NN	denotes	HCO3
T930	2858-2859	SYM	denotes	-
T931	2860-2863	CC	denotes	and
T932	2864-2871	NN	denotes	formate
T933	2872-2873	-LRB-	denotes	[
T935	2873-2874	CD	denotes	6
T936	2874-2875	,	denotes	,
T934	2875-2876	CD	denotes	7
T937	2876-2877	-RRB-	denotes	]
T938	2877-2878	.	denotes	.
T939	2878-3006	sentence	denotes	Expression has been found in the inner ear and thyroid gland consistent with the clinical signs of deafness and goiter [2,3,8].
T940	2879-2889	NN	denotes	Expression
T942	2890-2893	VBZ	denotes	has
T943	2894-2898	VBN	denotes	been
T941	2899-2904	VBN	denotes	found
T944	2905-2907	IN	denotes	in
T945	2908-2911	DT	denotes	the
T947	2912-2917	JJ	denotes	inner
T946	2918-2921	NN	denotes	ear
T948	2922-2925	CC	denotes	and
T949	2926-2933	NN	denotes	thyroid
T950	2934-2939	NN	denotes	gland
T951	2940-2950	JJ	denotes	consistent
T952	2951-2955	IN	denotes	with
T953	2956-2959	DT	denotes	the
T955	2960-2968	JJ	denotes	clinical
T954	2969-2974	NNS	denotes	signs
T956	2975-2977	IN	denotes	of
T957	2978-2986	NN	denotes	deafness
T958	2987-2990	CC	denotes	and
T959	2991-2997	NN	denotes	goiter
T960	2998-2999	-LRB-	denotes	[
T962	2999-3000	CD	denotes	2
T963	3000-3001	,	denotes	,
T964	3001-3002	CD	denotes	3
T965	3002-3003	,	denotes	,
T961	3003-3004	CD	denotes	8
T966	3004-3005	-RRB-	denotes	]
T967	3005-3006	.	denotes	.
T968	3006-3136	sentence	denotes	In addition, pendrin expression has been found in the kidney [9], mammary gland [10], uterus [11], testes [12] and placenta [13].
T969	3007-3009	IN	denotes	In
T971	3010-3018	NN	denotes	addition
T972	3018-3020	,	denotes	,
T973	3020-3027	NN	denotes	pendrin
T974	3028-3038	NN	denotes	expression
T975	3039-3042	VBZ	denotes	has
T976	3043-3047	VBN	denotes	been
T970	3048-3053	VBN	denotes	found
T977	3054-3056	IN	denotes	in
T978	3057-3060	DT	denotes	the
T979	3061-3067	NN	denotes	kidney
T980	3068-3069	-LRB-	denotes	[
T981	3069-3070	CD	denotes	9
T982	3070-3071	-RRB-	denotes	]
T983	3071-3073	,	denotes	,
T984	3073-3080	JJ	denotes	mammary
T985	3081-3086	NN	denotes	gland
T986	3087-3088	-LRB-	denotes	[
T987	3088-3090	CD	denotes	10
T988	3090-3091	-RRB-	denotes	]
T989	3091-3093	,	denotes	,
T990	3093-3099	NN	denotes	uterus
T991	3100-3101	-LRB-	denotes	[
T992	3101-3103	CD	denotes	11
T993	3103-3104	-RRB-	denotes	]
T994	3104-3106	,	denotes	,
T995	3106-3112	NNS	denotes	testes
T996	3113-3114	-LRB-	denotes	[
T997	3114-3116	CD	denotes	12
T998	3116-3117	-RRB-	denotes	]
T999	3118-3121	CC	denotes	and
T1000	3122-3130	NN	denotes	placenta
T1001	3131-3132	-LRB-	denotes	[
T1002	3132-3134	CD	denotes	13
T1003	3134-3135	-RRB-	denotes	]
T1004	3135-3136	.	denotes	.
T1005	3136-3240	sentence	denotes	No expression was found in fetal or adult brain, consistent with a peripheral cause of deafness [2,11].
T1006	3137-3139	DT	denotes	No
T1007	3140-3150	NN	denotes	expression
T1009	3151-3154	VBD	denotes	was
T1008	3155-3160	VBN	denotes	found
T1010	3161-3163	IN	denotes	in
T1011	3164-3169	JJ	denotes	fetal
T1013	3170-3172	CC	denotes	or
T1014	3173-3178	JJ	denotes	adult
T1012	3179-3184	NN	denotes	brain
T1015	3184-3186	,	denotes	,
T1016	3186-3196	JJ	denotes	consistent
T1017	3197-3201	IN	denotes	with
T1018	3202-3203	DT	denotes	a
T1020	3204-3214	JJ	denotes	peripheral
T1019	3215-3220	NN	denotes	cause
T1021	3221-3223	IN	denotes	of
T1022	3224-3232	NN	denotes	deafness
T1023	3233-3234	-LRB-	denotes	[
T1025	3234-3235	CD	denotes	2
T1026	3235-3236	,	denotes	,
T1024	3236-3238	CD	denotes	11
T1027	3238-3239	-RRB-	denotes	]
T1028	3239-3240	.	denotes	.
T1029	3240-3396	sentence	denotes	Expression of pendrin mRNA in the inner ear has been found in several places including the cochlea, the vestibular labyrinth and the endolymphatic sac [8].
T1030	3241-3251	NN	denotes	Expression
T1032	3252-3254	IN	denotes	of
T1033	3255-3262	NN	denotes	pendrin
T1034	3263-3267	NN	denotes	mRNA
T1035	3268-3270	IN	denotes	in
T1036	3271-3274	DT	denotes	the
T1038	3275-3280	JJ	denotes	inner
T1037	3281-3284	NN	denotes	ear
T1039	3285-3288	VBZ	denotes	has
T1040	3289-3293	VBN	denotes	been
T1031	3294-3299	VBN	denotes	found
T1041	3300-3302	IN	denotes	in
T1042	3303-3310	JJ	denotes	several
T1043	3311-3317	NNS	denotes	places
T1044	3318-3327	VBG	denotes	including
T1045	3328-3331	DT	denotes	the
T1046	3332-3339	NN	denotes	cochlea
T1047	3339-3341	,	denotes	,
T1048	3341-3344	DT	denotes	the
T1050	3345-3355	JJ	denotes	vestibular
T1049	3356-3365	NN	denotes	labyrinth
T1051	3366-3369	CC	denotes	and
T1052	3370-3373	DT	denotes	the
T1054	3374-3387	JJ	denotes	endolymphatic
T1053	3388-3391	NN	denotes	sac
T1055	3392-3393	-LRB-	denotes	[
T1056	3393-3394	CD	denotes	8
T1057	3394-3395	-RRB-	denotes	]
T1058	3395-3396	.	denotes	.
T1059	3396-3486	sentence	denotes	The precise location of pendrin protein expression, however, has not yet been determined.
T1060	3397-3400	DT	denotes	The
T1062	3401-3408	JJ	denotes	precise
T1061	3409-3417	NN	denotes	location
T1064	3418-3420	IN	denotes	of
T1065	3421-3428	NN	denotes	pendrin
T1067	3429-3436	NN	denotes	protein
T1066	3437-3447	NN	denotes	expression
T1068	3447-3449	,	denotes	,
T1069	3449-3456	RB	denotes	however
T1070	3456-3458	,	denotes	,
T1071	3458-3461	VBZ	denotes	has
T1072	3462-3465	RB	denotes	not
T1073	3466-3469	RB	denotes	yet
T1074	3470-3474	VBN	denotes	been
T1063	3475-3485	VBN	denotes	determined
T1075	3485-3486	.	denotes	.
T1076	3486-3669	sentence	denotes	The variability of deafness in Pendred syndrome and the observation that deafness is sometimes late in onset suggest that pendrin dysfunction may not be the direct cause of deafness.
T1077	3487-3490	DT	denotes	The
T1078	3491-3502	NN	denotes	variability
T1080	3503-3505	IN	denotes	of
T1081	3506-3514	NN	denotes	deafness
T1082	3515-3517	IN	denotes	in
T1083	3518-3525	NNP	denotes	Pendred
T1084	3526-3534	NN	denotes	syndrome
T1085	3535-3538	CC	denotes	and
T1086	3539-3542	DT	denotes	the
T1087	3543-3554	NN	denotes	observation
T1088	3555-3559	IN	denotes	that
T1090	3560-3568	NN	denotes	deafness
T1089	3569-3571	VBZ	denotes	is
T1091	3572-3581	RB	denotes	sometimes
T1092	3582-3586	JJ	denotes	late
T1093	3587-3589	IN	denotes	in
T1094	3590-3595	NN	denotes	onset
T1079	3596-3603	VBP	denotes	suggest
T1095	3604-3608	IN	denotes	that
T1097	3609-3616	NN	denotes	pendrin
T1098	3617-3628	NN	denotes	dysfunction
T1099	3629-3632	MD	denotes	may
T1100	3633-3636	RB	denotes	not
T1096	3637-3639	VB	denotes	be
T1101	3640-3643	DT	denotes	the
T1103	3644-3650	JJ	denotes	direct
T1102	3651-3656	NN	denotes	cause
T1104	3657-3659	IN	denotes	of
T1105	3660-3668	NN	denotes	deafness
T1106	3668-3669	.	denotes	.
T1107	3669-3827	sentence	denotes	It is conceivable that pendrin dysfunction favors changes in the expression levels of proteins that are critical for the maintenance of the hearing function.
T1108	3670-3672	PRP	denotes	It
T1109	3673-3675	VBZ	denotes	is
T1110	3676-3687	JJ	denotes	conceivable
T1111	3688-3692	IN	denotes	that
T1113	3693-3700	NN	denotes	pendrin
T1114	3701-3712	NN	denotes	dysfunction
T1112	3713-3719	VBZ	denotes	favors
T1115	3720-3727	NNS	denotes	changes
T1116	3728-3730	IN	denotes	in
T1117	3731-3734	DT	denotes	the
T1119	3735-3745	NN	denotes	expression
T1118	3746-3752	NNS	denotes	levels
T1120	3753-3755	IN	denotes	of
T1121	3756-3764	NN	denotes	proteins
T1122	3765-3769	WDT	denotes	that
T1123	3770-3773	VBP	denotes	are
T1124	3774-3782	JJ	denotes	critical
T1125	3783-3786	IN	denotes	for
T1126	3787-3790	DT	denotes	the
T1127	3791-3802	NN	denotes	maintenance
T1128	3803-3805	IN	denotes	of
T1129	3806-3809	DT	denotes	the
T1131	3810-3817	NN	denotes	hearing
T1130	3818-3826	NN	denotes	function
T1132	3826-3827	.	denotes	.
T1133	3827-4117	sentence	denotes	Detailed studies aimed at identifying the direct cause of deafness in Pendred syndrome have recently become possible due to the generation of a pendrin-specific polyclonal antibody [9] and the development of Slc26a4-/- mice, which bear a targeted disruption of the mouse Slc26a4 gene [14].
T1134	3828-3836	JJ	denotes	Detailed
T1135	3837-3844	NNS	denotes	studies
T1137	3845-3850	VBN	denotes	aimed
T1138	3851-3853	IN	denotes	at
T1139	3854-3865	VBG	denotes	identifying
T1140	3866-3869	DT	denotes	the
T1142	3870-3876	JJ	denotes	direct
T1141	3877-3882	NN	denotes	cause
T1143	3883-3885	IN	denotes	of
T1144	3886-3894	NN	denotes	deafness
T1145	3895-3897	IN	denotes	in
T1146	3898-3905	NNP	denotes	Pendred
T1147	3906-3914	NN	denotes	syndrome
T1148	3915-3919	VBP	denotes	have
T1149	3920-3928	RB	denotes	recently
T1136	3929-3935	VB	denotes	become
T1150	3936-3944	JJ	denotes	possible
T1151	3945-3948	IN	denotes	due
T1152	3949-3951	IN	denotes	to
T1153	3952-3955	DT	denotes	the
T1154	3956-3966	NN	denotes	generation
T1155	3967-3969	IN	denotes	of
T1156	3970-3971	DT	denotes	a
T1158	3972-3979	NN	denotes	pendrin
T1160	3979-3980	HYPH	denotes	-
T1159	3980-3988	JJ	denotes	specific
T1161	3989-3999	JJ	denotes	polyclonal
T1157	4000-4008	NN	denotes	antibody
T1162	4009-4010	-LRB-	denotes	[
T1163	4010-4011	CD	denotes	9
T1164	4011-4012	-RRB-	denotes	]
T1165	4013-4016	CC	denotes	and
T1166	4017-4020	DT	denotes	the
T1167	4021-4032	NN	denotes	development
T1168	4033-4035	IN	denotes	of
T1169	4036-4043	NN	denotes	Slc26a4
T1171	4043-4044	SYM	denotes	-
T1172	4044-4045	HYPH	denotes	/
T1173	4045-4046	SYM	denotes	-
T1170	4047-4051	NNS	denotes	mice
T1174	4051-4053	,	denotes	,
T1175	4053-4058	WDT	denotes	which
T1176	4059-4063	VBP	denotes	bear
T1177	4064-4065	DT	denotes	a
T1179	4066-4074	JJ	denotes	targeted
T1178	4075-4085	NN	denotes	disruption
T1180	4086-4088	IN	denotes	of
T1181	4089-4092	DT	denotes	the
T1183	4093-4098	NN	denotes	mouse
T1184	4099-4106	NN	denotes	Slc26a4
T1182	4107-4111	NN	denotes	gene
T1185	4112-4113	-LRB-	denotes	[
T1186	4113-4115	CD	denotes	14
T1187	4115-4116	-RRB-	denotes	]
T1188	4116-4117	.	denotes	.
T1189	4117-4233	sentence	denotes	The aim in the present study was to determine the location of pendrin and the cause of deafness in Slc26a4-/- mice.
T1190	4118-4121	DT	denotes	The
T1191	4122-4125	NN	denotes	aim
T1193	4126-4128	IN	denotes	in
T1194	4129-4132	DT	denotes	the
T1196	4133-4140	JJ	denotes	present
T1195	4141-4146	NN	denotes	study
T1192	4147-4150	VBD	denotes	was
T1197	4151-4153	TO	denotes	to
T1198	4154-4163	VB	denotes	determine
T1199	4164-4167	DT	denotes	the
T1200	4168-4176	NN	denotes	location
T1201	4177-4179	IN	denotes	of
T1202	4180-4187	NN	denotes	pendrin
T1203	4188-4191	CC	denotes	and
T1204	4192-4195	DT	denotes	the
T1205	4196-4201	NN	denotes	cause
T1206	4202-4204	IN	denotes	of
T1207	4205-4213	NN	denotes	deafness
T1208	4214-4216	IN	denotes	in
T1209	4217-4224	NN	denotes	Slc26a4
T1211	4224-4225	SYM	denotes	-
T1212	4225-4226	HYPH	denotes	/
T1213	4226-4227	SYM	denotes	-
T1210	4228-4232	NNS	denotes	mice
T1214	4232-4233	.	denotes	.
T1322	4292-4295	CC	denotes	and
T1323	4296-4309	JJ	denotes	perilymphatic
T1324	4310-4311	NN	denotes	K
T1325	4311-4312	SYM	denotes	+
T1320	4313-4327	NNS	denotes	concentrations
T1326	4328-4332	VBD	denotes	were
T1317	4333-4341	VBN	denotes	measured
T1327	4342-4344	IN	denotes	in
T1328	4345-4350	JJ	denotes	young
T1330	4351-4356	JJ	denotes	adult
T1329	4357-4361	NNS	denotes	mice
T1331	4362-4363	-LRB-	denotes	(
T1333	4363-4364	CD	denotes	1
T1335	4364-4365	SYM	denotes	–
T1334	4365-4366	CD	denotes	4
T1332	4367-4372	NN	denotes	month
T1336	4373-4375	IN	denotes	of
T1337	4376-4379	NN	denotes	age
T1338	4379-4380	-RRB-	denotes	)
T1339	4381-4385	WDT	denotes	that
T1341	4386-4392	CC	denotes	either
T1340	4393-4397	VBP	denotes	have
T1342	4398-4399	-LRB-	denotes	(
T1343	4399-4406	NN	denotes	Slc26a4
T1344	4406-4407	SYM	denotes	+
T1345	4407-4408	HYPH	denotes	/
T1346	4408-4409	SYM	denotes	+
T1347	4409-4410	-RRB-	denotes	)
T1348	4411-4413	CC	denotes	or
T1349	4414-4418	VBP	denotes	lack
T1350	4419-4420	-LRB-	denotes	(
T1351	4420-4427	NN	denotes	Slc26a4
T1352	4427-4428	SYM	denotes	-
T1353	4428-4429	HYPH	denotes	/
T1354	4429-4430	SYM	denotes	-
T1355	4430-4431	-RRB-	denotes	)
T1356	4432-4433	DT	denotes	a
T1358	4434-4444	JJ	denotes	functional
T1357	4445-4449	NN	denotes	gene
T1359	4450-4453	IN	denotes	for
T1360	4454-4461	NN	denotes	pendrin
T1361	4462-4463	-LRB-	denotes	[
T1362	4463-4465	CD	denotes	14
T1363	4465-4466	-RRB-	denotes	]
T1364	4466-4467	.	denotes	.
T1365	4467-4685	sentence	denotes	The mouse strain 129Sv/Ev (Taconic, Germantown, NY) was chosen as the source of Slc26a4+/+ controls, since Slc26a4-/- mice were propagated in the this strain and generated using a stem cell line derived from 129Sv/Ev.
T1366	4468-4471	DT	denotes	The
T1368	4472-4477	NN	denotes	mouse
T1367	4478-4484	NN	denotes	strain
T1370	4485-4490	NN	denotes	129Sv
T1372	4490-4491	HYPH	denotes	/
T1371	4491-4493	NN	denotes	Ev
T1373	4494-4495	-LRB-	denotes	(
T1374	4495-4502	NNP	denotes	Taconic
T1375	4502-4504	,	denotes	,
T1376	4504-4514	NNP	denotes	Germantown
T1377	4514-4516	,	denotes	,
T1378	4516-4518	NNP	denotes	NY
T1379	4518-4519	-RRB-	denotes	)
T1380	4520-4523	VBD	denotes	was
T1369	4524-4530	VBN	denotes	chosen
T1381	4531-4533	IN	denotes	as
T1382	4534-4537	DT	denotes	the
T1383	4538-4544	NN	denotes	source
T1384	4545-4547	IN	denotes	of
T1385	4548-4555	NN	denotes	Slc26a4
T1387	4555-4556	SYM	denotes	+
T1388	4556-4557	HYPH	denotes	/
T1389	4557-4558	SYM	denotes	+
T1386	4559-4567	NNS	denotes	controls
T1390	4567-4569	,	denotes	,
T1391	4569-4574	IN	denotes	since
T1393	4575-4582	NN	denotes	Slc26a4
T1395	4582-4583	SYM	denotes	-
T1396	4583-4584	HYPH	denotes	/
T1397	4584-4585	SYM	denotes	-
T1394	4586-4590	NNS	denotes	mice
T1398	4591-4595	VBD	denotes	were
T1392	4596-4606	VBN	denotes	propagated
T1399	4607-4609	IN	denotes	in
T1400	4610-4613	DT	denotes	the
T1402	4614-4618	DT	denotes	this
T1401	4619-4625	NN	denotes	strain
T1403	4626-4629	CC	denotes	and
T1404	4630-4639	VBN	denotes	generated
T1405	4640-4645	VBG	denotes	using
T1406	4646-4647	DT	denotes	a
T1408	4648-4652	NN	denotes	stem
T1409	4653-4657	NN	denotes	cell
T1407	4658-4662	NN	denotes	line
T1410	4663-4670	VBN	denotes	derived
T1411	4671-4675	IN	denotes	from
T1412	4676-4681	NN	denotes	129Sv
T1414	4681-4682	HYPH	denotes	/
T1413	4682-4684	NN	denotes	Ev
T1415	4684-4685	.	denotes	.
T1417	4686-4693	NN	denotes	Slc26a4
T1416	4686-4724	sentence	denotes	Slc26a4+/+ and Slc26a4-/- were agouti.
T1419	4693-4694	SYM	denotes	+
T1420	4694-4695	HYPH	denotes	/
T1421	4695-4696	SYM	denotes	+
T1422	4697-4700	CC	denotes	and
T1423	4701-4708	NN	denotes	Slc26a4
T1424	4708-4709	SYM	denotes	-
T1425	4709-4710	HYPH	denotes	/
T1426	4710-4711	SYM	denotes	-
T1418	4712-4716	VBD	denotes	were
T1427	4717-4723	NN	denotes	agouti
T1428	4723-4724	.	denotes	.
T1429	4724-4759	sentence	denotes	They did not differ in coat color.
T1430	4725-4729	PRP	denotes	They
T1432	4730-4733	VBD	denotes	did
T1433	4734-4737	RB	denotes	not
T1431	4738-4744	VB	denotes	differ
T1434	4745-4747	IN	denotes	in
T1435	4748-4752	NN	denotes	coat
T1436	4753-4758	NN	denotes	color
T1437	4758-4759	.	denotes	.
T1438	4759-4884	sentence	denotes	Differences in pigmentation were verified using coisogenic age-matched Slc26a4+/+ and Slc26a4-/- that were bred in parallel.
T1439	4760-4771	NNS	denotes	Differences
T1441	4772-4774	IN	denotes	in
T1442	4775-4787	NN	denotes	pigmentation
T1443	4788-4792	VBD	denotes	were
T1440	4793-4801	VBN	denotes	verified
T1444	4802-4807	VBG	denotes	using
T1445	4808-4818	JJ	denotes	coisogenic
T1447	4819-4822	NN	denotes	age
T1449	4822-4823	HYPH	denotes	-
T1448	4823-4830	VBN	denotes	matched
T1446	4831-4838	NN	denotes	Slc26a4
T1450	4838-4839	SYM	denotes	+
T1451	4839-4840	HYPH	denotes	/
T1452	4840-4841	SYM	denotes	+
T1453	4842-4845	CC	denotes	and
T1454	4846-4853	NN	denotes	Slc26a4
T1455	4853-4854	SYM	denotes	-
T1456	4854-4855	HYPH	denotes	/
T1457	4855-4856	SYM	denotes	-
T1458	4857-4861	WDT	denotes	that
T1460	4862-4866	VBD	denotes	were
T1459	4867-4871	VBN	denotes	bred
T1461	4872-4874	IN	denotes	in
T1462	4875-4883	NN	denotes	parallel
T1463	4883-4884	.	denotes	.
T1465	4885-4895	NN	denotes	Expression
T1464	4885-5064	sentence	denotes	Expression of key proteins involved in the generation of the endocochlear potential and the transport of K+ was studied using confocal immunocytochemistry and quantitative RT-PCR.
T1467	4896-4898	IN	denotes	of
T1468	4899-4902	JJ	denotes	key
T1469	4903-4911	NN	denotes	proteins
T1470	4912-4920	VBN	denotes	involved
T1471	4921-4923	IN	denotes	in
T1472	4924-4927	DT	denotes	the
T1473	4928-4938	NN	denotes	generation
T1474	4939-4941	IN	denotes	of
T1475	4942-4945	DT	denotes	the
T1477	4946-4958	JJ	denotes	endocochlear
T1476	4959-4968	NN	denotes	potential
T1478	4969-4972	CC	denotes	and
T1479	4973-4976	DT	denotes	the
T1480	4977-4986	NN	denotes	transport
T1481	4987-4989	IN	denotes	of
T1482	4990-4991	NN	denotes	K
T1483	4991-4992	SYM	denotes	+
T1484	4993-4996	VBD	denotes	was
T1466	4997-5004	VBN	denotes	studied
T1485	5005-5010	VBG	denotes	using
T1486	5011-5019	JJ	denotes	confocal
T1487	5020-5039	NN	denotes	immunocytochemistry
T1488	5040-5043	CC	denotes	and
T1489	5044-5056	JJ	denotes	quantitative
T1491	5057-5059	NN	denotes	RT
T1492	5059-5060	HYPH	denotes	-
T1490	5060-5063	NN	denotes	PCR
T1493	5063-5064	.	denotes	.
T1494	5064-5179	sentence	denotes	K+ secretion and the generation of the endocochlear potential were measured using electrophysiological techniques.
T1495	5065-5066	NN	denotes	K
T1497	5066-5067	SYM	denotes	+
T1496	5068-5077	NN	denotes	secretion
T1499	5078-5081	CC	denotes	and
T1500	5082-5085	DT	denotes	the
T1501	5086-5096	NN	denotes	generation
T1502	5097-5099	IN	denotes	of
T1503	5100-5103	DT	denotes	the
T1505	5104-5116	JJ	denotes	endocochlear
T1504	5117-5126	NN	denotes	potential
T1506	5127-5131	VBD	denotes	were
T1498	5132-5140	VBN	denotes	measured
T1507	5141-5146	VBG	denotes	using
T1508	5147-5167	JJ	denotes	electrophysiological
T1509	5168-5178	NNS	denotes	techniques
T1510	5178-5179	.	denotes	.
T1511	5179-5288	sentence	denotes	All experiments were approved by the Institutional Animal Care and Use Committee of Kansas State University.
T1512	5180-5183	DT	denotes	All
T1513	5184-5195	NNS	denotes	experiments
T1515	5196-5200	VBD	denotes	were
T1514	5201-5209	VBN	denotes	approved
T1516	5210-5212	IN	denotes	by
T1517	5213-5216	DT	denotes	the
T1519	5217-5230	NNP	denotes	Institutional
T1520	5231-5237	NNP	denotes	Animal
T1521	5238-5242	NNP	denotes	Care
T1522	5243-5246	CC	denotes	and
T1523	5247-5250	NNP	denotes	Use
T1518	5251-5260	NNP	denotes	Committee
T1524	5261-5263	IN	denotes	of
T1525	5264-5270	NNP	denotes	Kansas
T1527	5271-5276	NNP	denotes	State
T1526	5277-5287	NNP	denotes	University
T1528	5287-5288	.	denotes	.
T1678	5290-5298	JJ	denotes	Confocal
T1679	5299-5318	NN	denotes	immunocytochemistry
T1680	5318-5511	sentence	denotes	Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.) and transcardially perfused with a Cl--free phosphate-buffered Na-gluconate solution containing 4% paraformaldehyde.
T1681	5319-5326	NNS	denotes	Animals
T1683	5327-5331	VBD	denotes	were
T1684	5332-5338	RB	denotes	deeply
T1682	5339-5351	VBN	denotes	anesthetized
T1685	5352-5356	IN	denotes	with
T1686	5357-5363	NN	denotes	sodium
T1687	5364-5377	NN	denotes	pentobarbital
T1688	5378-5379	-LRB-	denotes	(
T1690	5379-5382	CD	denotes	100
T1689	5383-5385	NNS	denotes	mg
T1691	5385-5386	SYM	denotes	/
T1692	5386-5388	NN	denotes	kg
T1693	5389-5393	RB	denotes	i.p.
T1694	5393-5394	-RRB-	denotes	)
T1695	5395-5398	CC	denotes	and
T1696	5399-5413	RB	denotes	transcardially
T1697	5414-5422	VBN	denotes	perfused
T1698	5423-5427	IN	denotes	with
T1699	5428-5429	DT	denotes	a
T1701	5430-5432	NN	denotes	Cl
T1703	5432-5433	SYM	denotes	-
T1704	5433-5434	HYPH	denotes	-
T1702	5434-5438	JJ	denotes	free
T1705	5439-5448	NN	denotes	phosphate
T1707	5448-5449	HYPH	denotes	-
T1706	5449-5457	VBN	denotes	buffered
T1708	5458-5460	NN	denotes	Na
T1710	5460-5461	SYM	denotes	-
T1709	5461-5470	NN	denotes	gluconate
T1700	5471-5479	NN	denotes	solution
T1711	5480-5490	VBG	denotes	containing
T1712	5491-5492	CD	denotes	4
T1713	5492-5493	NN	denotes	%
T1714	5494-5510	NN	denotes	paraformaldehyde
T1715	5510-5511	.	denotes	.
T1716	5511-5686	sentence	denotes	Temporal bones were removed and cochleae fixed by perilymphatic perfusion, decalcified in EDTA, processed through a sucrose gradient and infiltrated with polyethylene glycol.
T1717	5512-5520	JJ	denotes	Temporal
T1718	5521-5526	NNS	denotes	bones
T1720	5527-5531	VBD	denotes	were
T1719	5532-5539	VBN	denotes	removed
T1721	5540-5543	CC	denotes	and
T1722	5544-5552	NNS	denotes	cochleae
T1723	5553-5558	VBN	denotes	fixed
T1724	5559-5561	IN	denotes	by
T1725	5562-5575	JJ	denotes	perilymphatic
T1726	5576-5585	NN	denotes	perfusion
T1727	5585-5587	,	denotes	,
T1728	5587-5598	VBN	denotes	decalcified
T1729	5599-5601	IN	denotes	in
T1730	5602-5606	NN	denotes	EDTA
T1731	5606-5608	,	denotes	,
T1732	5608-5617	VBN	denotes	processed
T1733	5618-5625	IN	denotes	through
T1734	5626-5627	DT	denotes	a
T1736	5628-5635	NN	denotes	sucrose
T1735	5636-5644	NN	denotes	gradient
T1737	5645-5648	CC	denotes	and
T1738	5649-5660	VBN	denotes	infiltrated
T1739	5661-5665	IN	denotes	with
T1740	5666-5678	NN	denotes	polyethylene
T1741	5679-5685	NN	denotes	glycol
T1742	5685-5686	.	denotes	.
T1743	5686-5833	sentence	denotes	Mid-modiolar cryosections (12 μm, CM3050S, Leica, Nussloch, Germany) were blocked in PBS with 0.2% Triton-X (PBS-TX) and 10% bovine serum albumin.
T1744	5687-5699	JJ	denotes	Mid-modiolar
T1745	5700-5712	NNS	denotes	cryosections
T1747	5713-5714	-LRB-	denotes	(
T1749	5714-5716	CD	denotes	12
T1750	5717-5719	NNS	denotes	μm
T1751	5719-5721	,	denotes	,
T1748	5721-5728	NN	denotes	CM3050S
T1752	5728-5730	,	denotes	,
T1753	5730-5735	NNP	denotes	Leica
T1754	5735-5737	,	denotes	,
T1755	5737-5745	NNP	denotes	Nussloch
T1756	5745-5747	,	denotes	,
T1757	5747-5754	NNP	denotes	Germany
T1758	5754-5755	-RRB-	denotes	)
T1759	5756-5760	VBD	denotes	were
T1746	5761-5768	VBN	denotes	blocked
T1760	5769-5771	IN	denotes	in
T1761	5772-5775	NN	denotes	PBS
T1762	5776-5780	IN	denotes	with
T1763	5781-5784	CD	denotes	0.2
T1764	5784-5785	NN	denotes	%
T1766	5786-5792	NN	denotes	Triton
T1767	5792-5793	HYPH	denotes	-
T1765	5793-5794	NN	denotes	X
T1768	5795-5796	-LRB-	denotes	(
T1770	5796-5799	NN	denotes	PBS
T1771	5799-5800	HYPH	denotes	-
T1769	5800-5802	NN	denotes	TX
T1772	5802-5803	-RRB-	denotes	)
T1773	5804-5807	CC	denotes	and
T1774	5808-5810	CD	denotes	10
T1775	5810-5811	NN	denotes	%
T1777	5812-5818	JJ	denotes	bovine
T1778	5819-5824	NN	denotes	serum
T1776	5825-5832	NN	denotes	albumin
T1779	5832-5833	.	denotes	.
T1780	5833-6274	sentence	denotes	Slides were incubated overnight at 4°C with primary antibodies in PBS-TX with 1–3% BSA [rabbit anti-pendrin, 1:500 (h766–780); rat anti-ZO-1, 1:100 (Chemicon, Temecula, CA); goat anti-KCNQ1, 1:200 (C20, Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-KCNE1, 1:200 (Alomone, Jerusalem, Israel); rabbit anti-KCNJ10, 1:300 (Alomone); rabbit anti-SLC12A2, 1:100 (Chemicon); and rabbit anti-connexin 26, 1:100 (Zymed, San Francisco, CA)].
T1781	5834-5840	NNS	denotes	Slides
T1783	5841-5845	VBD	denotes	were
T1782	5846-5855	VBN	denotes	incubated
T1784	5856-5865	RB	denotes	overnight
T1785	5866-5868	IN	denotes	at
T1786	5869-5870	CD	denotes	4
T1787	5870-5872	NN	denotes	°C
T1788	5873-5877	IN	denotes	with
T1789	5878-5885	JJ	denotes	primary
T1790	5886-5896	NNS	denotes	antibodies
T1791	5897-5899	IN	denotes	in
T1792	5900-5903	NN	denotes	PBS
T1794	5903-5904	HYPH	denotes	-
T1793	5904-5906	NN	denotes	TX
T1795	5907-5911	IN	denotes	with
T1796	5912-5913	CD	denotes	1
T1798	5913-5914	SYM	denotes	–
T1797	5914-5915	CD	denotes	3
T1799	5915-5916	NN	denotes	%
T1800	5917-5920	NN	denotes	BSA
T1801	5921-5922	-LRB-	denotes	[
T1803	5922-5928	NN	denotes	rabbit
T1804	5929-5941	NN	denotes	anti-pendrin
T1805	5941-5943	,	denotes	,
T1806	5943-5944	CD	denotes	1
T1807	5944-5945	SYM	denotes	:
T1808	5945-5948	CD	denotes	500
T1809	5949-5950	-LRB-	denotes	(
T1802	5950-5954	NN	denotes	h766
T1810	5954-5955	SYM	denotes	–
T1811	5955-5958	CD	denotes	780
T1812	5958-5959	-RRB-	denotes	)
T1813	5959-5960	,	denotes	;
T1814	5961-5964	NN	denotes	rat
T1815	5965-5972	NN	denotes	anti-ZO
T1817	5972-5973	HYPH	denotes	-
T1818	5973-5974	CD	denotes	1
T1819	5974-5976	,	denotes	,
T1820	5976-5977	CD	denotes	1
T1821	5977-5978	SYM	denotes	:
T1822	5978-5981	CD	denotes	100
T1823	5982-5983	-LRB-	denotes	(
T1816	5983-5991	NNP	denotes	Chemicon
T1824	5991-5993	,	denotes	,
T1825	5993-6001	NNP	denotes	Temecula
T1826	6001-6003	,	denotes	,
T1827	6003-6005	NNP	denotes	CA
T1828	6005-6006	-RRB-	denotes	)
T1829	6006-6007	,	denotes	;
T1830	6008-6012	NN	denotes	goat
T1831	6013-6023	NN	denotes	anti-KCNQ1
T1833	6023-6025	,	denotes	,
T1834	6025-6026	CD	denotes	1
T1835	6026-6027	SYM	denotes	:
T1836	6027-6030	CD	denotes	200
T1837	6031-6032	-LRB-	denotes	(
T1832	6032-6035	NN	denotes	C20
T1838	6035-6037	,	denotes	,
T1839	6037-6042	NNP	denotes	Santa
T1840	6043-6047	NNP	denotes	Cruz
T1841	6048-6061	NNP	denotes	Biotechnology
T1842	6061-6063	,	denotes	,
T1843	6063-6068	NNP	denotes	Santa
T1844	6069-6073	NNP	denotes	Cruz
T1845	6073-6075	,	denotes	,
T1846	6075-6077	NNP	denotes	CA
T1847	6077-6078	-RRB-	denotes	)
T1848	6078-6080	,	denotes	,
T1849	6080-6086	NN	denotes	rabbit
T1850	6087-6097	NN	denotes	anti-KCNE1
T1852	6097-6099	,	denotes	,
T1853	6099-6100	CD	denotes	1
T1854	6100-6101	SYM	denotes	:
T1855	6101-6104	CD	denotes	200
T1856	6105-6106	-LRB-	denotes	(
T1851	6106-6113	NNP	denotes	Alomone
T1857	6113-6115	,	denotes	,
T1858	6115-6124	NNP	denotes	Jerusalem
T1859	6124-6126	,	denotes	,
T1860	6126-6132	NNP	denotes	Israel
T1861	6132-6133	-RRB-	denotes	)
T1862	6133-6134	,	denotes	;
T1863	6135-6141	NN	denotes	rabbit
T1864	6142-6153	NN	denotes	anti-KCNJ10
T1866	6153-6155	,	denotes	,
T1867	6155-6156	CD	denotes	1
T1868	6156-6157	SYM	denotes	:
T1869	6157-6160	CD	denotes	300
T1870	6161-6162	-LRB-	denotes	(
T1865	6162-6169	NNP	denotes	Alomone
T1871	6169-6170	-RRB-	denotes	)
T1872	6170-6171	,	denotes	;
T1873	6172-6178	NN	denotes	rabbit
T1874	6179-6191	NN	denotes	anti-SLC12A2
T1876	6191-6193	,	denotes	,
T1877	6193-6194	CD	denotes	1
T1878	6194-6195	SYM	denotes	:
T1879	6195-6198	CD	denotes	100
T1880	6199-6200	-LRB-	denotes	(
T1875	6200-6208	NNP	denotes	Chemicon
T1881	6208-6209	-RRB-	denotes	)
T1882	6209-6210	:	denotes	;
T1883	6211-6214	CC	denotes	and
T1884	6215-6221	NN	denotes	rabbit
T1885	6222-6235	NN	denotes	anti-connexin
T1887	6236-6238	CD	denotes	26
T1888	6238-6240	,	denotes	,
T1889	6240-6241	CD	denotes	1
T1890	6241-6242	SYM	denotes	:
T1891	6242-6245	CD	denotes	100
T1892	6246-6247	-LRB-	denotes	(
T1886	6247-6252	NNP	denotes	Zymed
T1893	6252-6254	,	denotes	,
T1894	6254-6257	NNP	denotes	San
T1895	6258-6267	NNP	denotes	Francisco
T1896	6267-6269	,	denotes	,
T1897	6269-6271	NNP	denotes	CA
T1898	6271-6272	-RRB-	denotes	)
T1899	6272-6273	-RRB-	denotes	]
T1900	6273-6274	.	denotes	.
T1901	6274-6540	sentence	denotes	Slides were washed in PBS-TX and incubated for 1 h at 25°C with appropriate secondary antibodies at a 1:1000 dilution in PBS-TX with 1–3% BSA [donkey anti-rabbit Alexa 488, chicken anti-rat Alexa 594, and chicken anti-goat Alexa 594 (Molecular Probes, Eugene, OR)].
T1902	6275-6281	NNS	denotes	Slides
T1904	6282-6286	VBD	denotes	were
T1903	6287-6293	VBN	denotes	washed
T1905	6294-6296	IN	denotes	in
T1906	6297-6300	NN	denotes	PBS
T1908	6300-6301	HYPH	denotes	-
T1907	6301-6303	NN	denotes	TX
T1909	6304-6307	CC	denotes	and
T1910	6308-6317	VBN	denotes	incubated
T1911	6318-6321	IN	denotes	for
T1912	6322-6323	CD	denotes	1
T1913	6324-6325	NN	denotes	h
T1914	6326-6328	IN	denotes	at
T1915	6329-6331	CD	denotes	25
T1916	6331-6333	NN	denotes	°C
T1917	6334-6338	IN	denotes	with
T1918	6339-6350	JJ	denotes	appropriate
T1920	6351-6360	JJ	denotes	secondary
T1919	6361-6371	NNS	denotes	antibodies
T1921	6372-6374	IN	denotes	at
T1922	6375-6376	DT	denotes	a
T1924	6377-6378	CD	denotes	1
T1925	6378-6379	SYM	denotes	:
T1926	6379-6383	CD	denotes	1000
T1923	6384-6392	NN	denotes	dilution
T1927	6393-6395	IN	denotes	in
T1928	6396-6399	NN	denotes	PBS
T1930	6399-6400	HYPH	denotes	-
T1929	6400-6402	NN	denotes	TX
T1931	6403-6407	IN	denotes	with
T1932	6408-6409	CD	denotes	1
T1934	6409-6410	SYM	denotes	–
T1933	6410-6411	CD	denotes	3
T1935	6411-6412	NN	denotes	%
T1936	6413-6416	NN	denotes	BSA
T1937	6417-6418	-LRB-	denotes	[
T1939	6418-6424	NN	denotes	donkey
T1941	6425-6436	JJ	denotes	anti-rabbit
T1940	6437-6442	NNP	denotes	Alexa
T1942	6443-6446	CD	denotes	488
T1943	6446-6448	,	denotes	,
T1944	6448-6455	NN	denotes	chicken
T1946	6456-6464	JJ	denotes	anti-rat
T1945	6465-6470	NNP	denotes	Alexa
T1947	6471-6474	CD	denotes	594
T1948	6474-6476	,	denotes	,
T1949	6476-6479	CC	denotes	and
T1950	6480-6487	NN	denotes	chicken
T1952	6488-6497	JJ	denotes	anti-goat
T1951	6498-6503	NNP	denotes	Alexa
T1953	6504-6507	CD	denotes	594
T1954	6508-6509	-LRB-	denotes	(
T1955	6509-6518	NNP	denotes	Molecular
T1938	6519-6525	NNP	denotes	Probes
T1956	6525-6527	,	denotes	,
T1957	6527-6533	NNP	denotes	Eugene
T1958	6533-6535	,	denotes	,
T1959	6535-6537	NNP	denotes	OR
T1960	6537-6538	-RRB-	denotes	)
T1961	6538-6539	-RRB-	denotes	]
T1962	6539-6540	.	denotes	.
T1963	6540-6641	sentence	denotes	Actin filaments were visualized by staining with Alexa 488 conjugated phalloidin (Molecular Probes).
T1964	6541-6546	NN	denotes	Actin
T1965	6547-6556	NNS	denotes	filaments
T1967	6557-6561	VBD	denotes	were
T1966	6562-6572	VBN	denotes	visualized
T1968	6573-6575	IN	denotes	by
T1969	6576-6584	VBG	denotes	staining
T1970	6585-6589	IN	denotes	with
T1971	6590-6595	NNP	denotes	Alexa
T1973	6596-6599	CD	denotes	488
T1972	6600-6610	VBN	denotes	conjugated
T1974	6611-6621	NN	denotes	phalloidin
T1975	6622-6623	-LRB-	denotes	(
T1977	6623-6632	NNP	denotes	Molecular
T1976	6633-6639	NNP	denotes	Probes
T1978	6639-6640	-RRB-	denotes	)
T1979	6640-6641	.	denotes	.
T1980	6641-6848	sentence	denotes	After incubation, slides were washed with PBS-TX, mounted with FluorSave (Calbiochem, La Jolla, CA), and viewed by confocal microscopy (LSM 5 Pascal or LSM 510 Meta, Carl Zeiss, Jena or Göttingen, Germany).
T1981	6642-6647	IN	denotes	After
T1983	6648-6658	NN	denotes	incubation
T1984	6658-6660	,	denotes	,
T1985	6660-6666	NNS	denotes	slides
T1986	6667-6671	VBD	denotes	were
T1982	6672-6678	VBN	denotes	washed
T1987	6679-6683	IN	denotes	with
T1988	6684-6687	NN	denotes	PBS
T1990	6687-6688	HYPH	denotes	-
T1989	6688-6690	NN	denotes	TX
T1991	6690-6692	,	denotes	,
T1992	6692-6699	VBN	denotes	mounted
T1993	6700-6704	IN	denotes	with
T1994	6705-6714	NNP	denotes	FluorSave
T1995	6715-6716	-LRB-	denotes	(
T1996	6716-6726	NNP	denotes	Calbiochem
T1997	6726-6728	,	denotes	,
T1998	6728-6730	NNP	denotes	La
T1999	6731-6736	NNP	denotes	Jolla
T2000	6736-6738	,	denotes	,
T2001	6738-6740	NNP	denotes	CA
T2002	6740-6741	-RRB-	denotes	)
T2003	6741-6743	,	denotes	,
T2004	6743-6746	CC	denotes	and
T2005	6747-6753	VBN	denotes	viewed
T2006	6754-6756	IN	denotes	by
T2007	6757-6765	JJ	denotes	confocal
T2008	6766-6776	NN	denotes	microscopy
T2009	6777-6778	-LRB-	denotes	(
T2011	6778-6781	NN	denotes	LSM
T2012	6782-6783	CD	denotes	5
T2010	6784-6790	NN	denotes	Pascal
T2013	6791-6793	CC	denotes	or
T2014	6794-6797	NN	denotes	LSM
T2016	6798-6801	CD	denotes	510
T2015	6802-6806	NN	denotes	Meta
T2017	6806-6808	,	denotes	,
T2018	6808-6812	NNP	denotes	Carl
T2019	6813-6818	NNP	denotes	Zeiss
T2020	6818-6820	,	denotes	,
T2021	6820-6824	NNP	denotes	Jena
T2022	6825-6827	CC	denotes	or
T2023	6828-6837	NNP	denotes	Göttingen
T2024	6837-6839	,	denotes	,
T2025	6839-6846	NNP	denotes	Germany
T2026	6846-6847	-RRB-	denotes	)
T2027	6847-6848	.	denotes	.
T2028	6848-6994	sentence	denotes	Laser scanning brightfield images were collected to document structural preservation, for morphometric analysis and for analysis of pigmentation.
T2029	6849-6854	NN	denotes	Laser
T2030	6855-6863	NN	denotes	scanning
T2032	6864-6875	NN	denotes	brightfield
T2031	6876-6882	NNS	denotes	images
T2034	6883-6887	VBD	denotes	were
T2033	6888-6897	VBN	denotes	collected
T2035	6898-6900	TO	denotes	to
T2036	6901-6909	VB	denotes	document
T2037	6910-6920	JJ	denotes	structural
T2038	6921-6933	NN	denotes	preservation
T2039	6933-6935	,	denotes	,
T2040	6935-6938	IN	denotes	for
T2041	6939-6951	JJ	denotes	morphometric
T2042	6952-6960	NN	denotes	analysis
T2043	6961-6964	CC	denotes	and
T2044	6965-6968	IN	denotes	for
T2045	6969-6977	NN	denotes	analysis
T2046	6978-6980	IN	denotes	of
T2047	6981-6993	NN	denotes	pigmentation
T2048	6993-6994	.	denotes	.
T2210	6996-7008	JJ	denotes	Quantitative
T2212	7009-7011	NN	denotes	RT
T2213	7011-7012	HYPH	denotes	-
T2211	7012-7015	NN	denotes	PCR
T2214	7015-7092	sentence	denotes	Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.).
T2215	7016-7023	NNS	denotes	Animals
T2217	7024-7028	VBD	denotes	were
T2218	7029-7035	RB	denotes	deeply
T2216	7036-7048	VBN	denotes	anesthetized
T2219	7049-7053	IN	denotes	with
T2220	7054-7060	NN	denotes	sodium
T2221	7061-7074	NN	denotes	pentobarbital
T2222	7075-7076	-LRB-	denotes	(
T2224	7076-7079	CD	denotes	100
T2223	7080-7082	NN	denotes	mg
T2225	7082-7083	SYM	denotes	/
T2226	7083-7085	NN	denotes	kg
T2227	7086-7090	RB	denotes	i.p.
T2228	7090-7091	-RRB-	denotes	)
T2229	7091-7092	.	denotes	.
T2230	7092-7152	sentence	denotes	Kidneys and brain were removed and pulverized in liquid N2.
T2231	7093-7100	NNS	denotes	Kidneys
T2233	7101-7104	CC	denotes	and
T2234	7105-7110	NN	denotes	brain
T2235	7111-7115	VBD	denotes	were
T2232	7116-7123	VBN	denotes	removed
T2236	7124-7127	CC	denotes	and
T2237	7128-7138	VBN	denotes	pulverized
T2238	7139-7141	IN	denotes	in
T2239	7142-7148	NN	denotes	liquid
T2240	7149-7151	NN	denotes	N2
T2241	7151-7152	.	denotes	.
T2242	7152-7380	sentence	denotes	Temporal bones were removed and two preparations of inner ear tissues were obtained by microdissection: (1) stria vascularis without spiral ligament, and (2) spiral ganglia including the surrounding bone and the organ of Corti.
T2243	7153-7161	JJ	denotes	Temporal
T2244	7162-7167	NNS	denotes	bones
T2246	7168-7172	VBD	denotes	were
T2245	7173-7180	VBN	denotes	removed
T2247	7181-7184	CC	denotes	and
T2248	7185-7188	CD	denotes	two
T2249	7189-7201	NNS	denotes	preparations
T2251	7202-7204	IN	denotes	of
T2252	7205-7210	JJ	denotes	inner
T2254	7211-7214	NN	denotes	ear
T2253	7215-7222	NNS	denotes	tissues
T2255	7223-7227	VBD	denotes	were
T2250	7228-7236	VBN	denotes	obtained
T2256	7237-7239	IN	denotes	by
T2257	7240-7255	NN	denotes	microdissection
T2258	7255-7257	:	denotes	:
T2259	7257-7258	-LRB-	denotes	(
T2260	7258-7259	LS	denotes	1
T2262	7259-7260	-RRB-	denotes	)
T2263	7261-7266	NN	denotes	stria
T2261	7267-7277	NN	denotes	vascularis
T2264	7278-7285	IN	denotes	without
T2265	7286-7292	JJ	denotes	spiral
T2266	7293-7301	NN	denotes	ligament
T2267	7301-7303	,	denotes	,
T2268	7303-7306	CC	denotes	and
T2269	7307-7308	-LRB-	denotes	(
T2270	7308-7309	LS	denotes	2
T2272	7309-7310	-RRB-	denotes	)
T2273	7311-7317	JJ	denotes	spiral
T2271	7318-7325	NNS	denotes	ganglia
T2274	7326-7335	VBG	denotes	including
T2275	7336-7339	DT	denotes	the
T2277	7340-7351	VBG	denotes	surrounding
T2276	7352-7356	NN	denotes	bone
T2278	7357-7360	CC	denotes	and
T2279	7361-7364	DT	denotes	the
T2280	7365-7370	NN	denotes	organ
T2281	7371-7373	IN	denotes	of
T2282	7374-7379	NNP	denotes	Corti
T2283	7379-7380	.	denotes	.
T2284	7380-7525	sentence	denotes	The dissection medium was changed twice during the microdissection and isolated tissues were washed three times to minimize cross-contamination.
T2285	7381-7384	DT	denotes	The
T2287	7385-7395	NN	denotes	dissection
T2286	7396-7402	NN	denotes	medium
T2289	7403-7406	VBD	denotes	was
T2288	7407-7414	VBN	denotes	changed
T2290	7415-7420	RB	denotes	twice
T2291	7421-7427	IN	denotes	during
T2292	7428-7431	DT	denotes	the
T2293	7432-7447	NN	denotes	microdissection
T2294	7448-7451	CC	denotes	and
T2295	7452-7460	VBN	denotes	isolated
T2296	7461-7468	NNS	denotes	tissues
T2298	7469-7473	VBD	denotes	were
T2297	7474-7480	VBN	denotes	washed
T2299	7481-7486	CD	denotes	three
T2300	7487-7492	NNS	denotes	times
T2301	7493-7495	TO	denotes	to
T2302	7496-7504	VB	denotes	minimize
T2303	7505-7524	NN	denotes	cross-contamination
T2304	7524-7525	.	denotes	.
T2305	7525-7628	sentence	denotes	The Slc26a4-/-genotype was verified by the observation of large otoconia in the utricular macula [14].
T2306	7526-7529	DT	denotes	The
T2308	7530-7537	NN	denotes	Slc26a4
T2309	7537-7538	SYM	denotes	-
T2310	7538-7539	HYPH	denotes	/
T2311	7539-7540	SYM	denotes	-
T2307	7540-7548	NN	denotes	genotype
T2313	7549-7552	VBD	denotes	was
T2312	7553-7561	VBN	denotes	verified
T2314	7562-7564	IN	denotes	by
T2315	7565-7568	DT	denotes	the
T2316	7569-7580	NN	denotes	observation
T2317	7581-7583	IN	denotes	of
T2318	7584-7589	JJ	denotes	large
T2319	7590-7598	NN	denotes	otoconia
T2320	7599-7601	IN	denotes	in
T2321	7602-7605	DT	denotes	the
T2323	7606-7615	JJ	denotes	utricular
T2322	7616-7622	NN	denotes	macula
T2324	7623-7624	-LRB-	denotes	[
T2325	7624-7626	CD	denotes	14
T2326	7626-7627	-RRB-	denotes	]
T2327	7627-7628	.	denotes	.
T2328	7628-7755	sentence	denotes	Total RNA was isolated and residual DNA contamination was removed by enzymatic digestion (RNeasy micro, Qiagen, Valencia, CA).
T2329	7629-7634	JJ	denotes	Total
T2330	7635-7638	NN	denotes	RNA
T2332	7639-7642	VBD	denotes	was
T2331	7643-7651	VBN	denotes	isolated
T2333	7652-7655	CC	denotes	and
T2334	7656-7664	JJ	denotes	residual
T2336	7665-7668	NN	denotes	DNA
T2335	7669-7682	NN	denotes	contamination
T2338	7683-7686	VBD	denotes	was
T2337	7687-7694	VBN	denotes	removed
T2339	7695-7697	IN	denotes	by
T2340	7698-7707	JJ	denotes	enzymatic
T2341	7708-7717	NN	denotes	digestion
T2342	7718-7719	-LRB-	denotes	(
T2344	7719-7725	NN	denotes	RNeasy
T2343	7726-7731	NN	denotes	micro
T2345	7731-7733	,	denotes	,
T2346	7733-7739	NNP	denotes	Qiagen
T2347	7739-7741	,	denotes	,
T2348	7741-7749	NNP	denotes	Valencia
T2349	7749-7751	,	denotes	,
T2350	7751-7753	NNP	denotes	CA
T2351	7753-7754	-RRB-	denotes	)
T2352	7754-7755	.	denotes	.
T2353	7755-7892	sentence	denotes	Quality and quantity of 18S rRNA obtained from kidneys and brain were determined (Nano Assay, 2100 Bioanalyzer, Agilent, Palo Alto, CA).
T2354	7756-7763	NN	denotes	Quality
T2356	7764-7767	CC	denotes	and
T2357	7768-7776	NN	denotes	quantity
T2358	7777-7779	IN	denotes	of
T2359	7780-7783	NN	denotes	18S
T2360	7784-7788	NN	denotes	rRNA
T2361	7789-7797	VBN	denotes	obtained
T2362	7798-7802	IN	denotes	from
T2363	7803-7810	NNS	denotes	kidneys
T2364	7811-7814	CC	denotes	and
T2365	7815-7820	NN	denotes	brain
T2366	7821-7825	VBD	denotes	were
T2355	7826-7836	VBN	denotes	determined
T2367	7837-7838	-LRB-	denotes	(
T2369	7838-7842	NNP	denotes	Nano
T2368	7843-7848	NNP	denotes	Assay
T2370	7848-7850	,	denotes	,
T2371	7850-7854	CD	denotes	2100
T2372	7855-7866	NNP	denotes	Bioanalyzer
T2373	7866-7868	,	denotes	,
T2374	7868-7875	NNP	denotes	Agilent
T2375	7875-7877	,	denotes	,
T2376	7877-7881	NNP	denotes	Palo
T2377	7882-7886	NNP	denotes	Alto
T2378	7886-7888	,	denotes	,
T2379	7888-7890	NNP	denotes	CA
T2380	7890-7891	-RRB-	denotes	)
T2381	7891-7892	.	denotes	.
T2382	7892-8028	sentence	denotes	Further, the quality of RNA preparations obtained from stria vascularis and spiral ganglia was assessed (Pico Assay, 2100 Bioanalyzer).
T2383	7893-7900	RB	denotes	Further
T2385	7900-7902	,	denotes	,
T2386	7902-7905	DT	denotes	the
T2387	7906-7913	NN	denotes	quality
T2388	7914-7916	IN	denotes	of
T2389	7917-7920	NN	denotes	RNA
T2390	7921-7933	NNS	denotes	preparations
T2391	7934-7942	VBN	denotes	obtained
T2392	7943-7947	IN	denotes	from
T2393	7948-7953	NN	denotes	stria
T2394	7954-7964	NN	denotes	vascularis
T2395	7965-7968	CC	denotes	and
T2396	7969-7975	JJ	denotes	spiral
T2397	7976-7983	NNS	denotes	ganglia
T2398	7984-7987	VBD	denotes	was
T2384	7988-7996	VBN	denotes	assessed
T2399	7997-7998	-LRB-	denotes	(
T2401	7998-8002	NNP	denotes	Pico
T2400	8003-8008	NNP	denotes	Assay
T2402	8008-8010	,	denotes	,
T2403	8010-8014	CD	denotes	2100
T2404	8015-8026	NNP	denotes	Bioanalyzer
T2405	8026-8027	-RRB-	denotes	)
T2406	8027-8028	.	denotes	.
T2407	8028-8219	sentence	denotes	Real time RT-PCR was performed on RNA obtained from individual animals (OneStep RT-PCR, Qiagen; Smart Cycler, Cepheid, Sunnyvale, CA) in the presence of 0.2× SYBR green I (Molecular Probes).
T2408	8029-8033	JJ	denotes	Real
T2409	8034-8038	NN	denotes	time
T2411	8039-8041	NN	denotes	RT
T2412	8041-8042	HYPH	denotes	-
T2410	8042-8045	NN	denotes	PCR
T2414	8046-8049	VBD	denotes	was
T2413	8050-8059	VBN	denotes	performed
T2415	8060-8062	IN	denotes	on
T2416	8063-8066	NN	denotes	RNA
T2417	8067-8075	VBN	denotes	obtained
T2418	8076-8080	IN	denotes	from
T2419	8081-8091	JJ	denotes	individual
T2420	8092-8099	NNS	denotes	animals
T2421	8100-8101	-LRB-	denotes	(
T2423	8101-8108	NNP	denotes	OneStep
T2425	8109-8111	NN	denotes	RT
T2426	8111-8112	HYPH	denotes	-
T2424	8112-8115	NN	denotes	PCR
T2427	8115-8117	,	denotes	,
T2428	8117-8123	NNP	denotes	Qiagen
T2429	8123-8124	:	denotes	;
T2430	8125-8130	NNP	denotes	Smart
T2422	8131-8137	NNP	denotes	Cycler
T2431	8137-8139	,	denotes	,
T2432	8139-8146	NNP	denotes	Cepheid
T2433	8146-8148	,	denotes	,
T2434	8148-8157	NNP	denotes	Sunnyvale
T2435	8157-8159	,	denotes	,
T2436	8159-8161	NNP	denotes	CA
T2437	8161-8162	-RRB-	denotes	)
T2438	8163-8165	IN	denotes	in
T2439	8166-8169	DT	denotes	the
T2440	8170-8178	NN	denotes	presence
T2441	8179-8181	IN	denotes	of
T2442	8182-8185	CD	denotes	0.2
T2444	8185-8186	SYM	denotes	×
T2443	8187-8191	NN	denotes	SYBR
T2446	8192-8197	JJ	denotes	green
T2445	8198-8199	NN	denotes	I
T2447	8200-8201	-LRB-	denotes	(
T2449	8201-8210	NNP	denotes	Molecular
T2448	8211-8217	NNP	denotes	Probes
T2450	8217-8218	-RRB-	denotes	)
T2451	8218-8219	.	denotes	.
T2452	8219-8350	sentence	denotes	Transcripts of 18S rRNA and mRNA for the K+ channels KCNJ10, KCNQ1 and KCNQ4 were amplified using gene-specific primers (Table 1).
T2453	8220-8231	NNS	denotes	Transcripts
T2455	8232-8234	IN	denotes	of
T2456	8235-8238	NN	denotes	18S
T2457	8239-8243	NN	denotes	rRNA
T2458	8244-8247	CC	denotes	and
T2459	8248-8252	NN	denotes	mRNA
T2460	8253-8256	IN	denotes	for
T2461	8257-8260	DT	denotes	the
T2463	8261-8262	NN	denotes	K
T2464	8262-8263	SYM	denotes	+
T2462	8264-8272	NNS	denotes	channels
T2465	8273-8279	NN	denotes	KCNJ10
T2466	8279-8281	,	denotes	,
T2467	8281-8286	NN	denotes	KCNQ1
T2468	8287-8290	CC	denotes	and
T2469	8291-8296	NN	denotes	KCNQ4
T2470	8297-8301	VBD	denotes	were
T2454	8302-8311	VBN	denotes	amplified
T2471	8312-8317	VBG	denotes	using
T2472	8318-8322	NN	denotes	gene
T2474	8322-8323	HYPH	denotes	-
T2473	8323-8331	JJ	denotes	specific
T2475	8332-8339	NNS	denotes	primers
T2476	8340-8341	-LRB-	denotes	(
T2477	8341-8346	NN	denotes	Table
T2478	8347-8348	CD	denotes	1
T2479	8348-8349	-RRB-	denotes	)
T2480	8349-8350	.	denotes	.
T2481	8350-8406	sentence	denotes	RT was performed for 30 min at 50°C and 15 min at 95°C.
T2482	8351-8353	NN	denotes	RT
T2484	8354-8357	VBD	denotes	was
T2483	8358-8367	VBN	denotes	performed
T2485	8368-8371	IN	denotes	for
T2486	8372-8374	CD	denotes	30
T2487	8375-8378	NN	denotes	min
T2488	8379-8381	IN	denotes	at
T2489	8382-8384	CD	denotes	50
T2490	8384-8386	NN	denotes	°C
T2491	8387-8390	CC	denotes	and
T2492	8391-8393	CD	denotes	15
T2493	8394-8397	NN	denotes	min
T2494	8398-8400	IN	denotes	at
T2495	8401-8403	CD	denotes	95
T2496	8403-8405	NN	denotes	°C
T2497	8405-8406	.	denotes	.
T2498	8406-8601	sentence	denotes	PCR consisted of 50 cycles of 1 min at 60°C, 1 min at 72°C, 7s heating to hot-measurement temperature, 13s hot-measurement at 3–5°C below product melting temperature (Table 1) and 1 min at 94°C.
T2499	8407-8410	NN	denotes	PCR
T2500	8411-8420	VBD	denotes	consisted
T2501	8421-8423	IN	denotes	of
T2502	8424-8426	CD	denotes	50
T2503	8427-8433	NNS	denotes	cycles
T2504	8434-8436	IN	denotes	of
T2505	8437-8438	CD	denotes	1
T2506	8439-8442	NN	denotes	min
T2507	8443-8445	IN	denotes	at
T2508	8446-8448	CD	denotes	60
T2509	8448-8450	NN	denotes	°C
T2510	8450-8452	,	denotes	,
T2511	8452-8453	CD	denotes	1
T2512	8454-8457	NN	denotes	min
T2513	8458-8460	IN	denotes	at
T2514	8461-8463	CD	denotes	72
T2515	8463-8465	NN	denotes	°C
T2516	8465-8467	,	denotes	,
T2517	8467-8469	NN	denotes	7s
T2518	8470-8477	VBG	denotes	heating
T2519	8478-8480	IN	denotes	to
T2520	8481-8484	JJ	denotes	hot
T2522	8484-8485	HYPH	denotes	-
T2521	8485-8496	NN	denotes	measurement
T2523	8497-8508	NN	denotes	temperature
T2524	8508-8510	,	denotes	,
T2525	8510-8513	NNS	denotes	13s
T2527	8514-8517	NN	denotes	hot
T2528	8517-8518	HYPH	denotes	-
T2526	8518-8529	NN	denotes	measurement
T2529	8530-8532	IN	denotes	at
T2530	8533-8534	CD	denotes	3
T2532	8534-8535	SYM	denotes	–
T2531	8535-8536	CD	denotes	5
T2533	8536-8538	NN	denotes	°C
T2534	8539-8544	IN	denotes	below
T2535	8545-8552	NN	denotes	product
T2537	8553-8560	NN	denotes	melting
T2536	8561-8572	NN	denotes	temperature
T2538	8573-8574	-LRB-	denotes	(
T2539	8574-8579	NN	denotes	Table
T2540	8580-8581	CD	denotes	1
T2541	8581-8582	-RRB-	denotes	)
T2542	8583-8586	CC	denotes	and
T2543	8587-8588	CD	denotes	1
T2544	8589-8592	NN	denotes	min
T2545	8593-8595	IN	denotes	at
T2546	8596-8598	CD	denotes	94
T2547	8598-8600	NN	denotes	°C
T2548	8600-8601	.	denotes	.
T2549	8601-8733	sentence	denotes	Hot-measurements were performed to eliminate the detection of primer-dimers that had melting temperatures between 72 and 75°C [15].
T2550	8602-8605	JJ	denotes	Hot
T2552	8605-8606	HYPH	denotes	-
T2551	8606-8618	NNS	denotes	measurements
T2554	8619-8623	VBD	denotes	were
T2553	8624-8633	VBN	denotes	performed
T2555	8634-8636	TO	denotes	to
T2556	8637-8646	VB	denotes	eliminate
T2557	8647-8650	DT	denotes	the
T2558	8651-8660	NN	denotes	detection
T2559	8661-8663	IN	denotes	of
T2560	8664-8670	NN	denotes	primer
T2562	8670-8671	HYPH	denotes	-
T2561	8671-8677	NNS	denotes	dimers
T2563	8678-8682	WDT	denotes	that
T2564	8683-8686	VBD	denotes	had
T2565	8687-8694	NN	denotes	melting
T2566	8695-8707	NNS	denotes	temperatures
T2567	8708-8715	IN	denotes	between
T2568	8716-8718	CD	denotes	72
T2570	8719-8722	CC	denotes	and
T2571	8723-8725	CD	denotes	75
T2569	8725-8727	NN	denotes	°C
T2572	8728-8729	-LRB-	denotes	[
T2573	8729-8731	CD	denotes	15
T2574	8731-8732	-RRB-	denotes	]
T2575	8732-8733	.	denotes	.
T2576	8733-8774	sentence	denotes	PCR was followed by a melt (60 to 95°C).
T2577	8734-8737	NN	denotes	PCR
T2579	8738-8741	VBD	denotes	was
T2578	8742-8750	VBN	denotes	followed
T2580	8751-8753	IN	denotes	by
T2581	8754-8755	DT	denotes	a
T2582	8756-8760	NN	denotes	melt
T2583	8761-8762	-LRB-	denotes	(
T2585	8762-8764	CD	denotes	60
T2587	8765-8767	IN	denotes	to
T2586	8768-8770	CD	denotes	95
T2584	8770-8772	NN	denotes	°C
T2588	8772-8773	-RRB-	denotes	)
T2589	8773-8774	.	denotes	.
T2590	8774-8925	sentence	denotes	The generation of a single product of appropriate size was routinely checked by the presence of a single melt peak and by agarose gel-electrophoresis.
T2591	8775-8778	DT	denotes	The
T2592	8779-8789	NN	denotes	generation
T2594	8790-8792	IN	denotes	of
T2595	8793-8794	DT	denotes	a
T2597	8795-8801	JJ	denotes	single
T2596	8802-8809	NN	denotes	product
T2598	8810-8812	IN	denotes	of
T2599	8813-8824	JJ	denotes	appropriate
T2600	8825-8829	NN	denotes	size
T2601	8830-8833	VBD	denotes	was
T2602	8834-8843	RB	denotes	routinely
T2593	8844-8851	VBN	denotes	checked
T2603	8852-8854	IN	denotes	by
T2604	8855-8858	DT	denotes	the
T2605	8859-8867	NN	denotes	presence
T2606	8868-8870	IN	denotes	of
T2607	8871-8872	DT	denotes	a
T2609	8873-8879	JJ	denotes	single
T2610	8880-8884	NN	denotes	melt
T2608	8885-8889	NN	denotes	peak
T2611	8890-8893	CC	denotes	and
T2612	8894-8896	IN	denotes	by
T2613	8897-8904	NN	denotes	agarose
T2614	8905-8908	NN	denotes	gel
T2616	8908-8909	HYPH	denotes	-
T2615	8909-8924	NN	denotes	electrophoresis
T2617	8924-8925	.	denotes	.
T2618	8925-8971	sentence	denotes	Product identity was confirmed by sequencing.
T2619	8926-8933	NN	denotes	Product
T2620	8934-8942	NN	denotes	identity
T2622	8943-8946	VBD	denotes	was
T2621	8947-8956	VBN	denotes	confirmed
T2623	8957-8959	IN	denotes	by
T2624	8960-8970	NN	denotes	sequencing
T2625	8970-8971	.	denotes	.
T2626	8971-9809	sentence	denotes	Table 1 Primers Template Primers Product Length Melting Temperature Hot Measurement Fidelity n 18S gag gtt cga aga cga tca ga (sense) 316 bp 83.2 ± 02°C 78°C 1.89 ± 0.02 17 tcg ctc cac caa cta aga ac (antisense) KCNJ10 tgg tgt ggt gtg gta tct gg (sense) 411 bp 83.2 ± 02°C 78°C 1.86 ± 0.02 15 tga agc agt ttg cct gtc ac (antisense) KCNQ1 ttt gtt cat ccc cat ctc ag (sense) 239 bp 82.5 ± 02°C 79°C 1.85 ± 0.02 17 gtt gct ggg tag gaa gag (antisense) KCNQ4 ccc gga aac cct tct gtg tc (sense) 245 bp 83.2 ± 02°C 80°C 1.87 ± 0.01 17 aaa gat gag cac cag gaa cc (antisense) Template molecules (T) were quantified according to T = P / (F ^Ct) where P is the number of product molecules, F is the fidelity of the reaction and Ct is the cycle at which the number of product molecules reaches a chosen threshold [15].
T2627	8972-8997	NN	denotes	Table 1 Primers Template
T7759	8981-8988	NNS	denotes	Primers
T2628	9579-9588	NNS	denotes	molecules
T2630	9589-9590	-LRB-	denotes	(
T2631	9590-9591	NN	denotes	T
T2632	9591-9592	-RRB-	denotes	)
T2633	9593-9597	VBD	denotes	were
T2629	9598-9608	VBN	denotes	quantified
T2634	9609-9618	VBG	denotes	according
T2635	9619-9621	IN	denotes	to
T2636	9622-9623	NN	denotes	T
T2638	9624-9625	SYM	denotes	=
T2637	9626-9627	NN	denotes	P
T2639	9628-9629	SYM	denotes	/
T2641	9630-9631	-LRB-	denotes	(
T2642	9631-9632	NN	denotes	F
T2643	9633-9634	SYM	denotes	^
T2640	9634-9636	NN	denotes	Ct
T2644	9636-9637	-RRB-	denotes	)
T2645	9638-9643	WRB	denotes	where
T2647	9644-9645	NN	denotes	P
T2646	9646-9648	VBZ	denotes	is
T2648	9649-9652	DT	denotes	the
T2649	9653-9659	NN	denotes	number
T2650	9660-9662	IN	denotes	of
T2651	9663-9670	NN	denotes	product
T2652	9671-9680	NNS	denotes	molecules
T2653	9680-9682	,	denotes	,
T2654	9682-9683	NN	denotes	F
T2655	9684-9686	VBZ	denotes	is
T2656	9687-9690	DT	denotes	the
T2657	9691-9699	NN	denotes	fidelity
T2658	9700-9702	IN	denotes	of
T2659	9703-9706	DT	denotes	the
T2660	9707-9715	NN	denotes	reaction
T2661	9716-9719	CC	denotes	and
T2662	9720-9722	NN	denotes	Ct
T2663	9723-9725	VBZ	denotes	is
T2664	9726-9729	DT	denotes	the
T2665	9730-9735	NN	denotes	cycle
T2666	9736-9738	IN	denotes	at
T2668	9739-9744	WDT	denotes	which
T2669	9745-9748	DT	denotes	the
T2670	9749-9755	NN	denotes	number
T2671	9756-9758	IN	denotes	of
T2672	9759-9766	NN	denotes	product
T2673	9767-9776	NNS	denotes	molecules
T2667	9777-9784	VBZ	denotes	reaches
T2674	9785-9786	DT	denotes	a
T2676	9787-9793	VBN	denotes	chosen
T2675	9794-9803	NN	denotes	threshold
T2677	9804-9805	-LRB-	denotes	[
T2678	9805-9807	CD	denotes	15
T2679	9807-9808	-RRB-	denotes	]
T2680	9808-9809	.	denotes	.
T2681	9809-10021	sentence	denotes	Fidelity (F) was obtained from the slope (S) of the log-linear phase of the growth curve via a best-fit fifth-order polynomial: F = 7.39 + 3.80 × S + 1.05 × S2 + 0.15 × S3 + 11.38 × 10-3 * S4 + 3.39 × 10-4 × S5.
T2682	9810-9818	NN	denotes	Fidelity
T2684	9819-9820	-LRB-	denotes	(
T2685	9820-9821	NN	denotes	F
T2686	9821-9822	-RRB-	denotes	)
T2687	9823-9826	VBD	denotes	was
T2683	9827-9835	VBN	denotes	obtained
T2688	9836-9840	IN	denotes	from
T2689	9841-9844	DT	denotes	the
T2690	9845-9850	NN	denotes	slope
T2691	9851-9852	-LRB-	denotes	(
T2692	9852-9853	NN	denotes	S
T2693	9853-9854	-RRB-	denotes	)
T2694	9855-9857	IN	denotes	of
T2695	9858-9861	DT	denotes	the
T2697	9862-9865	NN	denotes	log
T2699	9865-9866	SYM	denotes	-
T2698	9866-9872	JJ	denotes	linear
T2696	9873-9878	NN	denotes	phase
T2700	9879-9881	IN	denotes	of
T2701	9882-9885	DT	denotes	the
T2703	9886-9892	NN	denotes	growth
T2702	9893-9898	NN	denotes	curve
T2704	9899-9902	IN	denotes	via
T2705	9903-9904	DT	denotes	a
T2707	9905-9909	JJ	denotes	best
T2709	9909-9910	HYPH	denotes	-
T2708	9910-9913	NN	denotes	fit
T2710	9914-9919	JJ	denotes	fifth
T2712	9919-9920	HYPH	denotes	-
T2711	9920-9925	NN	denotes	order
T2706	9926-9936	NN	denotes	polynomial
T2713	9936-9938	:	denotes	:
T2714	9938-9939	NN	denotes	F
T2716	9940-9941	SYM	denotes	=
T2715	9942-9946	CD	denotes	7.39
T2717	9947-9948	SYM	denotes	+
T2718	9949-9953	CD	denotes	3.80
T2720	9954-9955	SYM	denotes	×
T2719	9956-9957	NN	denotes	S
T2721	9958-9959	SYM	denotes	+
T2722	9960-9964	CD	denotes	1.05
T2724	9965-9966	SYM	denotes	×
T2723	9967-9969	NN	denotes	S2
T2725	9970-9971	SYM	denotes	+
T2726	9972-9976	CD	denotes	0.15
T2728	9977-9978	SYM	denotes	×
T2727	9979-9981	NN	denotes	S3
T2729	9982-9983	SYM	denotes	+
T2730	9984-9989	CD	denotes	11.38
T2732	9990-9991	SYM	denotes	×
T2733	9992-9994	CD	denotes	10
T2734	9994-9995	HYPH	denotes	-
T2731	9995-9996	CD	denotes	3
T2736	9997-9998	SYM	denotes	*
T2735	9999-10001	NN	denotes	S4
T2737	10002-10003	SYM	denotes	+
T2738	10004-10008	CD	denotes	3.39
T2740	10009-10010	SYM	denotes	×
T2741	10011-10013	CD	denotes	10
T2742	10013-10014	HYPH	denotes	-
T2739	10014-10015	CD	denotes	4
T2744	10016-10017	SYM	denotes	×
T2743	10018-10020	NN	denotes	S5
T2745	10020-10021	.	denotes	.
T2746	10021-10163	sentence	denotes	The number of product molecules at threshold (PCt) was determined by amplification of known amounts of 18S rRNA according to PCt = T × F ^Ct.
T2747	10022-10025	DT	denotes	The
T2748	10026-10032	NN	denotes	number
T2750	10033-10035	IN	denotes	of
T2751	10036-10043	NN	denotes	product
T2752	10044-10053	NNS	denotes	molecules
T2753	10054-10056	IN	denotes	at
T2754	10057-10066	NN	denotes	threshold
T2755	10067-10068	-LRB-	denotes	(
T2756	10068-10071	NN	denotes	PCt
T2757	10071-10072	-RRB-	denotes	)
T2758	10073-10076	VBD	denotes	was
T2749	10077-10087	VBN	denotes	determined
T2759	10088-10090	IN	denotes	by
T2760	10091-10104	NN	denotes	amplification
T2761	10105-10107	IN	denotes	of
T2762	10108-10113	VBN	denotes	known
T2763	10114-10121	NNS	denotes	amounts
T2764	10122-10124	IN	denotes	of
T2765	10125-10128	NN	denotes	18S
T2766	10129-10133	NN	denotes	rRNA
T2767	10134-10143	VBG	denotes	according
T2768	10144-10146	IN	denotes	to
T2769	10147-10150	NN	denotes	PCt
T2771	10151-10152	SYM	denotes	=
T2770	10153-10154	NN	denotes	T
T2772	10155-10156	SYM	denotes	×
T2773	10157-10158	NN	denotes	F
T2775	10159-10160	SYM	denotes	^
T2774	10160-10162	NN	denotes	Ct
T2776	10162-10163	.	denotes	.
T2777	10163-10228	sentence	denotes	Quantifications of 18S rRNA were used to compare tissue amounts.
T2778	10164-10179	NNS	denotes	Quantifications
T2780	10180-10182	IN	denotes	of
T2781	10183-10186	NN	denotes	18S
T2782	10187-10191	NN	denotes	rRNA
T2783	10192-10196	VBD	denotes	were
T2779	10197-10201	VBN	denotes	used
T2784	10202-10204	TO	denotes	to
T2785	10205-10212	VB	denotes	compare
T2786	10213-10219	NN	denotes	tissue
T2787	10220-10227	NNS	denotes	amounts
T2788	10227-10228	.	denotes	.
T2789	10228-10325	sentence	denotes	Genomic contamination of inner ear samples was assessed to be <0.02% by omission of the RT step.
T2790	10229-10236	JJ	denotes	Genomic
T2791	10237-10250	NN	denotes	contamination
T2793	10251-10253	IN	denotes	of
T2794	10254-10259	JJ	denotes	inner
T2795	10260-10263	NN	denotes	ear
T2796	10264-10271	NNS	denotes	samples
T2797	10272-10275	VBD	denotes	was
T2792	10276-10284	VBN	denotes	assessed
T2798	10285-10287	TO	denotes	to
T2799	10288-10290	VB	denotes	be
T2800	10291-10292	SYM	denotes	<
T2802	10292-10296	CD	denotes	0.02
T2801	10296-10297	NN	denotes	%
T2803	10298-10300	IN	denotes	by
T2804	10301-10309	NN	denotes	omission
T2805	10310-10312	IN	denotes	of
T2806	10313-10316	DT	denotes	the
T2808	10317-10319	NN	denotes	RT
T2807	10320-10324	NN	denotes	step
T2809	10324-10325	.	denotes	.
T2833	10327-10329	FW	denotes	In
T2834	10330-10335	FW	denotes	vitro
T2835	10336-10353	NN	denotes	electrophysiology
T2836	10353-10430	sentence	denotes	Animals were deeply anesthetized with sodium pentobarbital (100 mg/kg i.p.).
T2837	10354-10361	NNS	denotes	Animals
T2839	10362-10366	VBD	denotes	were
T2840	10367-10373	RB	denotes	deeply
T2838	10374-10386	VBN	denotes	anesthetized
T2841	10387-10391	IN	denotes	with
T2842	10392-10398	NN	denotes	sodium
T2843	10399-10412	NN	denotes	pentobarbital
T2844	10413-10414	-LRB-	denotes	(
T2846	10414-10417	CD	denotes	100
T2845	10418-10420	NNS	denotes	mg
T2847	10420-10421	SYM	denotes	/
T2848	10421-10423	NN	denotes	kg
T2849	10424-10428	RB	denotes	i.p.
T2850	10428-10429	-RRB-	denotes	)
T2851	10429-10430	.	denotes	.
T2852	10430-10504	sentence	denotes	Stria vascularis without spiral ligament was obtained by microdissection.
T2853	10431-10436	NN	denotes	Stria
T2854	10437-10447	NN	denotes	vascularis
T2856	10448-10455	IN	denotes	without
T2857	10456-10462	JJ	denotes	spiral
T2858	10463-10471	NN	denotes	ligament
T2859	10472-10475	VBD	denotes	was
T2855	10476-10484	VBN	denotes	obtained
T2860	10485-10487	IN	denotes	by
T2861	10488-10503	NN	denotes	microdissection
T2862	10503-10504	.	denotes	.
T2863	10504-10576	sentence	denotes	Currents generated by the stria marginal epithelium were recorded [16].
T2864	10505-10513	NNS	denotes	Currents
T2866	10514-10523	VBN	denotes	generated
T2867	10524-10526	IN	denotes	by
T2868	10527-10530	DT	denotes	the
T2870	10531-10536	NN	denotes	stria
T2871	10537-10545	JJ	denotes	marginal
T2869	10546-10556	NN	denotes	epithelium
T2872	10557-10561	VBD	denotes	were
T2865	10562-10570	VBN	denotes	recorded
T2873	10571-10572	-LRB-	denotes	[
T2874	10572-10574	CD	denotes	16
T2875	10574-10575	-RRB-	denotes	]
T2876	10575-10576	.	denotes	.
T2877	10576-10848	sentence	denotes	A Pt-Ir wire microelectrode with a Pt-black tip was positioned 20–30 μm from the apical surface of the epithelium and vibrated at 200–400 Hz by piezo-electric bimorph elements (Applicable Electronics, Forest Dale, MA; ASET version 1.05, Science Wares, East Falmouth, MA).
T2878	10577-10578	DT	denotes	A
T2880	10579-10581	NN	denotes	Pt
T2882	10581-10582	HYPH	denotes	-
T2881	10582-10584	NN	denotes	Ir
T2883	10585-10589	NN	denotes	wire
T2879	10590-10604	NN	denotes	microelectrode
T2885	10605-10609	IN	denotes	with
T2886	10610-10611	DT	denotes	a
T2888	10612-10614	NN	denotes	Pt
T2889	10614-10615	HYPH	denotes	-
T2890	10615-10620	JJ	denotes	black
T2887	10621-10624	NN	denotes	tip
T2891	10625-10628	VBD	denotes	was
T2884	10629-10639	VBN	denotes	positioned
T2892	10640-10642	CD	denotes	20
T2894	10642-10643	HYPH	denotes	–
T2893	10643-10645	CD	denotes	30
T2895	10646-10648	NN	denotes	μm
T2896	10649-10653	IN	denotes	from
T2897	10654-10657	DT	denotes	the
T2899	10658-10664	JJ	denotes	apical
T2898	10665-10672	NN	denotes	surface
T2900	10673-10675	IN	denotes	of
T2901	10676-10679	DT	denotes	the
T2902	10680-10690	NN	denotes	epithelium
T2903	10691-10694	CC	denotes	and
T2904	10695-10703	VBN	denotes	vibrated
T2905	10704-10706	IN	denotes	at
T2906	10707-10710	CD	denotes	200
T2908	10710-10711	HYPH	denotes	–
T2907	10711-10714	CD	denotes	400
T2909	10715-10717	NN	denotes	Hz
T2910	10718-10720	IN	denotes	by
T2911	10721-10726	JJ	denotes	piezo
T2913	10726-10727	HYPH	denotes	-
T2912	10727-10735	JJ	denotes	electric
T2915	10736-10743	NN	denotes	bimorph
T2914	10744-10752	NNS	denotes	elements
T2916	10753-10754	-LRB-	denotes	(
T2918	10754-10764	NNP	denotes	Applicable
T2919	10765-10776	NNP	denotes	Electronics
T2920	10776-10778	,	denotes	,
T2921	10778-10784	NNP	denotes	Forest
T2922	10785-10789	NNP	denotes	Dale
T2923	10789-10791	,	denotes	,
T2924	10791-10793	NNP	denotes	MA
T2925	10793-10794	:	denotes	;
T2926	10795-10799	NN	denotes	ASET
T2917	10800-10807	NN	denotes	version
T2927	10808-10812	CD	denotes	1.05
T2928	10812-10814	,	denotes	,
T2929	10814-10821	NNP	denotes	Science
T2930	10822-10827	NNP	denotes	Wares
T2931	10827-10829	,	denotes	,
T2932	10829-10833	NNP	denotes	East
T2933	10834-10842	NNP	denotes	Falmouth
T2934	10842-10844	,	denotes	,
T2935	10844-10846	NNP	denotes	MA
T2936	10846-10847	-RRB-	denotes	)
T2937	10847-10848	.	denotes	.
T2938	10848-10921	sentence	denotes	A Pt-black electrode (26-gauge) served as reference in the bath chamber.
T2939	10849-10850	DT	denotes	A
T2941	10851-10853	NN	denotes	Pt
T2942	10853-10854	HYPH	denotes	-
T2943	10854-10859	JJ	denotes	black
T2940	10860-10869	NN	denotes	electrode
T2945	10870-10871	-LRB-	denotes	(
T2947	10871-10873	CD	denotes	26
T2948	10873-10874	HYPH	denotes	-
T2946	10874-10879	NN	denotes	gauge
T2949	10879-10880	-RRB-	denotes	)
T2944	10881-10887	VBD	denotes	served
T2950	10888-10890	IN	denotes	as
T2951	10891-10900	NN	denotes	reference
T2952	10901-10903	IN	denotes	in
T2953	10904-10907	DT	denotes	the
T2955	10908-10912	NN	denotes	bath
T2954	10913-10920	NN	denotes	chamber
T2956	10920-10921	.	denotes	.
T2957	10921-11013	sentence	denotes	The signals from the phase-sensitive detectors were digitized (16 bit) at a rate of 0.5 Hz.
T2958	10922-10925	DT	denotes	The
T2959	10926-10933	NNS	denotes	signals
T2961	10934-10938	IN	denotes	from
T2962	10939-10942	DT	denotes	the
T2964	10943-10948	NN	denotes	phase
T2966	10948-10949	HYPH	denotes	-
T2965	10949-10958	JJ	denotes	sensitive
T2963	10959-10968	NNS	denotes	detectors
T2967	10969-10973	VBD	denotes	were
T2960	10974-10983	VBN	denotes	digitized
T2968	10984-10985	-LRB-	denotes	(
T2970	10985-10987	CD	denotes	16
T2969	10988-10991	NN	denotes	bit
T2971	10991-10992	-RRB-	denotes	)
T2972	10993-10995	IN	denotes	at
T2973	10996-10997	DT	denotes	a
T2974	10998-11002	NN	denotes	rate
T2975	11003-11005	IN	denotes	of
T2976	11006-11009	CD	denotes	0.5
T2977	11010-11012	NN	denotes	Hz
T2978	11012-11013	.	denotes	.
T2979	11013-11075	sentence	denotes	The output was expressed as current density at the electrode.
T2980	11014-11017	DT	denotes	The
T2981	11018-11024	NN	denotes	output
T2983	11025-11028	VBD	denotes	was
T2982	11029-11038	VBN	denotes	expressed
T2984	11039-11041	IN	denotes	as
T2985	11042-11049	JJ	denotes	current
T2986	11050-11057	NN	denotes	density
T2987	11058-11060	IN	denotes	at
T2988	11061-11064	DT	denotes	the
T2989	11065-11074	NN	denotes	electrode
T2990	11074-11075	.	denotes	.
T3050	11077-11079	FW	denotes	In
T3051	11080-11084	FW	denotes	situ
T3052	11085-11102	NN	denotes	electrophysiology
T3053	11102-11187	sentence	denotes	Animals were anesthetized with inactin (thiobutabarbital sodium salt, 140 mg/kg ip).
T3054	11103-11110	NNS	denotes	Animals
T3056	11111-11115	VBD	denotes	were
T3055	11116-11128	VBN	denotes	anesthetized
T3057	11129-11133	IN	denotes	with
T3058	11134-11141	NN	denotes	inactin
T3059	11142-11143	-LRB-	denotes	(
T3061	11143-11159	NN	denotes	thiobutabarbital
T3063	11160-11166	NN	denotes	sodium
T3062	11167-11171	NN	denotes	salt
T3064	11171-11173	,	denotes	,
T3065	11173-11176	CD	denotes	140
T3060	11177-11179	NNS	denotes	mg
T3066	11179-11180	SYM	denotes	/
T3067	11180-11182	NN	denotes	kg
T3068	11183-11185	RB	denotes	ip
T3069	11185-11186	-RRB-	denotes	)
T3070	11186-11187	.	denotes	.
T3071	11187-11298	sentence	denotes	The endocochlear potential and the endolymphatic [K+] were measured with double-barreled microelectrodes [17].
T3072	11188-11191	DT	denotes	The
T3074	11192-11204	JJ	denotes	endocochlear
T3073	11205-11214	NN	denotes	potential
T3076	11215-11218	CC	denotes	and
T3077	11219-11222	DT	denotes	the
T3079	11223-11236	JJ	denotes	endolymphatic
T3080	11237-11238	-LRB-	denotes	[
T3078	11238-11239	NN	denotes	K
T3081	11239-11240	SYM	denotes	+
T3082	11240-11241	-RRB-	denotes	]
T3083	11242-11246	VBD	denotes	were
T3075	11247-11255	VBN	denotes	measured
T3084	11256-11260	IN	denotes	with
T3085	11261-11267	JJ	denotes	double
T3087	11267-11268	HYPH	denotes	-
T3086	11268-11276	JJ	denotes	barreled
T3088	11277-11292	NNS	denotes	microelectrodes
T3089	11293-11294	-LRB-	denotes	[
T3090	11294-11296	CD	denotes	17
T3091	11296-11297	-RRB-	denotes	]
T3092	11297-11298	.	denotes	.
T3093	11298-11503	sentence	denotes	Measurements were made in the basal turn by a round-window approach through the basilar membrane and in the apical turn after thinning the bone over the stria vascularis and picking a small hole (~30 μm).
T3094	11299-11311	NNS	denotes	Measurements
T3096	11312-11316	VBD	denotes	were
T3095	11317-11321	VBN	denotes	made
T3097	11322-11324	IN	denotes	in
T3098	11325-11328	DT	denotes	the
T3100	11329-11334	JJ	denotes	basal
T3099	11335-11339	NN	denotes	turn
T3101	11340-11342	IN	denotes	by
T3102	11343-11344	DT	denotes	a
T3104	11345-11350	JJ	denotes	round
T3106	11350-11351	HYPH	denotes	-
T3105	11351-11357	NN	denotes	window
T3103	11358-11366	NN	denotes	approach
T3107	11367-11374	IN	denotes	through
T3108	11375-11378	DT	denotes	the
T3110	11379-11386	JJ	denotes	basilar
T3109	11387-11395	NN	denotes	membrane
T3111	11396-11399	CC	denotes	and
T3112	11400-11402	IN	denotes	in
T3113	11403-11406	DT	denotes	the
T3115	11407-11413	JJ	denotes	apical
T3114	11414-11418	NN	denotes	turn
T3116	11419-11424	IN	denotes	after
T3117	11425-11433	VBG	denotes	thinning
T3118	11434-11437	DT	denotes	the
T3119	11438-11442	NN	denotes	bone
T3120	11443-11447	IN	denotes	over
T3121	11448-11451	DT	denotes	the
T3123	11452-11457	NN	denotes	stria
T3122	11458-11468	NN	denotes	vascularis
T3124	11469-11472	CC	denotes	and
T3125	11473-11480	VBG	denotes	picking
T3126	11481-11482	DT	denotes	a
T3128	11483-11488	JJ	denotes	small
T3127	11489-11493	NN	denotes	hole
T3129	11494-11495	-LRB-	denotes	(
T3131	11495-11496	SYM	denotes	~
T3132	11496-11498	CD	denotes	30
T3130	11499-11501	NNS	denotes	μm
T3133	11501-11502	-RRB-	denotes	)
T3134	11502-11503	.	denotes	.
T3135	11503-11613	sentence	denotes	K+-selective electrodes were calibrated in solutions of constant cation (K+ and Na+) concentration of 150 mM.
T3136	11504-11505	NN	denotes	K
T3138	11505-11506	SYM	denotes	+
T3139	11506-11507	HYPH	denotes	-
T3137	11507-11516	JJ	denotes	selective
T3140	11517-11527	NNS	denotes	electrodes
T3142	11528-11532	VBD	denotes	were
T3141	11533-11543	VBN	denotes	calibrated
T3143	11544-11546	IN	denotes	in
T3144	11547-11556	NNS	denotes	solutions
T3145	11557-11559	IN	denotes	of
T3146	11560-11568	JJ	denotes	constant
T3148	11569-11575	NN	denotes	cation
T3149	11576-11577	-LRB-	denotes	(
T3150	11577-11578	NN	denotes	K
T3151	11578-11579	SYM	denotes	+
T3152	11580-11583	CC	denotes	and
T3153	11584-11586	NN	denotes	Na
T3154	11586-11587	SYM	denotes	+
T3155	11587-11588	-RRB-	denotes	)
T3147	11589-11602	NN	denotes	concentration
T3156	11603-11605	IN	denotes	of
T3157	11606-11609	CD	denotes	150
T3158	11610-11612	NNS	denotes	mM
T3159	11612-11613	.	denotes	.
T3160	11613-11960	sentence	denotes	The minor selectivity of the K+ electrodes for Na+ produced a nonlinearity in the calibration curve, which was closely fitted by the Nicolski equation using nonlinear curve-fitting software (OriginLab, Northampton, MA): V = Vi + S × log ([K+] + A × [Na+]), where Vi is an offset term, S is slope, A is selectivity, and [Na+] is Na+ concentration.
T3161	11614-11617	DT	denotes	The
T3163	11618-11623	JJ	denotes	minor
T3162	11624-11635	NN	denotes	selectivity
T3165	11636-11638	IN	denotes	of
T3166	11639-11642	DT	denotes	the
T3168	11643-11644	NN	denotes	K
T3169	11644-11645	SYM	denotes	+
T3167	11646-11656	NNS	denotes	electrodes
T3170	11657-11660	IN	denotes	for
T3171	11661-11663	NN	denotes	Na
T3172	11663-11664	SYM	denotes	+
T3164	11665-11673	VBD	denotes	produced
T3173	11674-11675	DT	denotes	a
T3174	11676-11688	NN	denotes	nonlinearity
T3175	11689-11691	IN	denotes	in
T3176	11692-11695	DT	denotes	the
T3178	11696-11707	NN	denotes	calibration
T3177	11708-11713	NN	denotes	curve
T3179	11713-11715	,	denotes	,
T3180	11715-11720	WDT	denotes	which
T3182	11721-11724	VBD	denotes	was
T3183	11725-11732	RB	denotes	closely
T3181	11733-11739	VBN	denotes	fitted
T3184	11740-11742	IN	denotes	by
T3185	11743-11746	DT	denotes	the
T3187	11747-11755	NNP	denotes	Nicolski
T3186	11756-11764	NN	denotes	equation
T3188	11765-11770	VBG	denotes	using
T3189	11771-11780	JJ	denotes	nonlinear
T3191	11781-11786	NN	denotes	curve
T3192	11786-11787	HYPH	denotes	-
T3193	11787-11794	VBG	denotes	fitting
T3190	11795-11803	NN	denotes	software
T3194	11804-11805	-LRB-	denotes	(
T3195	11805-11814	NNP	denotes	OriginLab
T3196	11814-11816	,	denotes	,
T3197	11816-11827	NNP	denotes	Northampton
T3198	11827-11829	,	denotes	,
T3199	11829-11831	NNP	denotes	MA
T3200	11831-11832	-RRB-	denotes	)
T3201	11832-11834	:	denotes	:
T3202	11834-11835	NN	denotes	V
T3204	11836-11837	SYM	denotes	=
T3205	11838-11840	NN	denotes	Vi
T3206	11841-11842	SYM	denotes	+
T3207	11843-11844	NN	denotes	S
T3208	11845-11846	SYM	denotes	×
T3209	11847-11850	NN	denotes	log
T3210	11851-11852	-LRB-	denotes	(
T3211	11852-11853	-LRB-	denotes	[
T3203	11853-11854	NN	denotes	K
T3212	11854-11855	SYM	denotes	+
T3213	11855-11856	-RRB-	denotes	]
T3214	11857-11858	SYM	denotes	+
T3215	11859-11860	NN	denotes	A
T3217	11861-11862	SYM	denotes	×
T3218	11863-11864	-LRB-	denotes	[
T3216	11864-11866	NN	denotes	Na
T3219	11866-11867	SYM	denotes	+
T3220	11867-11868	-RRB-	denotes	]
T3221	11868-11869	-RRB-	denotes	)
T3222	11869-11871	,	denotes	,
T3223	11871-11876	WRB	denotes	where
T3225	11877-11879	NN	denotes	Vi
T3224	11880-11882	VBZ	denotes	is
T3226	11883-11885	DT	denotes	an
T3228	11886-11892	JJ	denotes	offset
T3227	11893-11897	NN	denotes	term
T3229	11897-11899	,	denotes	,
T3230	11899-11900	NN	denotes	S
T3231	11901-11903	VBZ	denotes	is
T3232	11904-11909	NN	denotes	slope
T3233	11909-11911	,	denotes	,
T3234	11911-11912	NN	denotes	A
T3235	11913-11915	VBZ	denotes	is
T3236	11916-11927	NN	denotes	selectivity
T3237	11927-11929	,	denotes	,
T3238	11929-11932	CC	denotes	and
T3239	11933-11934	-LRB-	denotes	[
T3240	11934-11936	NN	denotes	Na
T3242	11936-11937	SYM	denotes	+
T3243	11937-11938	-RRB-	denotes	]
T3241	11939-11941	VBZ	denotes	is
T3244	11942-11944	NN	denotes	Na
T3246	11944-11945	SYM	denotes	+
T3245	11946-11959	NN	denotes	concentration
T3247	11959-11960	.	denotes	.
T3248	11960-12048	sentence	denotes	Calibrations were made immediately after withdrawal of the electrodes from the cochlea.
T3249	11961-11973	NNS	denotes	Calibrations
T3251	11974-11978	VBD	denotes	were
T3250	11979-11983	VBN	denotes	made
T3252	11984-11995	RB	denotes	immediately
T3253	11996-12001	IN	denotes	after
T3254	12002-12012	NN	denotes	withdrawal
T3255	12013-12015	IN	denotes	of
T3256	12016-12019	DT	denotes	the
T3257	12020-12030	NNS	denotes	electrodes
T3258	12031-12035	IN	denotes	from
T3259	12036-12039	DT	denotes	the
T3260	12040-12047	NN	denotes	cochlea
T3261	12047-12048	.	denotes	.
T3262	12048-12158	sentence	denotes	Plasma K+ concentrations were obtained using a blood analyzer (Stat Profile M, Nova Biomedical, Waltham, MA).
T3263	12049-12055	NN	denotes	Plasma
T3265	12056-12057	NN	denotes	K
T3266	12057-12058	SYM	denotes	+
T3264	12059-12073	NNS	denotes	concentrations
T3268	12074-12078	VBD	denotes	were
T3267	12079-12087	VBN	denotes	obtained
T3269	12088-12093	VBG	denotes	using
T3270	12094-12095	DT	denotes	a
T3272	12096-12101	NN	denotes	blood
T3271	12102-12110	NN	denotes	analyzer
T3273	12111-12112	-LRB-	denotes	(
T3275	12112-12116	NNP	denotes	Stat
T3276	12117-12124	NNP	denotes	Profile
T3274	12125-12126	NNP	denotes	M
T3277	12126-12128	,	denotes	,
T3278	12128-12132	NNP	denotes	Nova
T3279	12133-12143	NNP	denotes	Biomedical
T3280	12143-12145	,	denotes	,
T3281	12145-12152	NNP	denotes	Waltham
T3282	12152-12154	,	denotes	,
T3283	12154-12156	NNP	denotes	MA
T3284	12156-12157	-RRB-	denotes	)
T3285	12157-12158	.	denotes	.
T3286	12158-12229	sentence	denotes	Data are presented as mean ± sem; n denotes the number of experiments.
T3287	12159-12163	NNS	denotes	Data
T3289	12164-12167	VBP	denotes	are
T3288	12168-12177	VBN	denotes	presented
T3291	12178-12180	IN	denotes	as
T3292	12181-12185	NN	denotes	mean
T3294	12186-12187	NN	denotes	±
T3293	12188-12191	NN	denotes	sem
T3295	12191-12192	:	denotes	;
T3296	12193-12194	NN	denotes	n
T3290	12195-12202	VBZ	denotes	denotes
T3297	12203-12206	DT	denotes	the
T3298	12207-12213	NN	denotes	number
T3299	12214-12216	IN	denotes	of
T3300	12217-12228	NNS	denotes	experiments
T3301	12228-12229	.	denotes	.
T3302	12229-12284	sentence	denotes	Differences were considered significant when p < 0.05.
T3303	12230-12241	NNS	denotes	Differences
T3305	12242-12246	VBD	denotes	were
T3304	12247-12257	VBN	denotes	considered
T3306	12258-12269	JJ	denotes	significant
T3307	12270-12274	WRB	denotes	when
T3309	12275-12276	NN	denotes	p
T3310	12277-12278	SYM	denotes	<
T3308	12279-12283	CD	denotes	0.05
T3311	12283-12284	.	denotes	.
T4701	12309-12311	FW	denotes	In
T4702	12312-12316	FW	denotes	situ
T4703	12317-12330	NN	denotes	hybridization
T4705	12331-12333	IN	denotes	in
T4706	12334-12337	DT	denotes	the
T4707	12338-12345	NN	denotes	cochlea
T4708	12346-12349	VBZ	denotes	has
T4704	12350-12359	VBN	denotes	suggested
T4709	12360-12364	IN	denotes	that
T4711	12365-12372	NN	denotes	pendrin
T4712	12373-12377	NN	denotes	mRNA
T4713	12378-12380	VBZ	denotes	is
T4710	12381-12390	VBN	denotes	expressed
T4714	12391-12393	IN	denotes	in
T4715	12394-12399	NNS	denotes	cells
T4716	12400-12404	WDT	denotes	that
T4717	12405-12411	VBP	denotes	reside
T4718	12412-12423	RB	denotes	immediately
T4719	12424-12431	IN	denotes	beneath
T4720	12432-12435	DT	denotes	the
T4722	12436-12442	JJ	denotes	spiral
T4721	12443-12453	NN	denotes	prominence
T4723	12454-12456	IN	denotes	on
T4724	12457-12460	DT	denotes	the
T4726	12461-12468	JJ	denotes	lateral
T4725	12469-12473	NN	denotes	wall
T4727	12474-12476	IN	denotes	of
T4728	12477-12480	DT	denotes	the
T4730	12481-12489	JJ	denotes	external
T4729	12490-12496	NN	denotes	sulcus
T4731	12497-12498	-LRB-	denotes	[
T4732	12498-12499	CD	denotes	8
T4733	12499-12500	-RRB-	denotes	]
T4734	12500-12501	.	denotes	.
T4735	12501-12708	sentence	denotes	To determine the location of pendrin protein expression, we performed confocal immunocytochemistry on cryosections prepared from temporal bones of normal (Slc26a4+/+) mice using an established antibody [3].
T4736	12502-12504	TO	denotes	To
T4737	12505-12514	VB	denotes	determine
T4739	12515-12518	DT	denotes	the
T4740	12519-12527	NN	denotes	location
T4741	12528-12530	IN	denotes	of
T4742	12531-12538	NN	denotes	pendrin
T4744	12539-12546	NN	denotes	protein
T4743	12547-12557	NN	denotes	expression
T4745	12557-12559	,	denotes	,
T4746	12559-12561	PRP	denotes	we
T4738	12562-12571	VBD	denotes	performed
T4747	12572-12580	JJ	denotes	confocal
T4748	12581-12600	NN	denotes	immunocytochemistry
T4749	12601-12603	IN	denotes	on
T4750	12604-12616	NNS	denotes	cryosections
T4751	12617-12625	VBN	denotes	prepared
T4752	12626-12630	IN	denotes	from
T4753	12631-12639	JJ	denotes	temporal
T4754	12640-12645	NNS	denotes	bones
T4755	12646-12648	IN	denotes	of
T4756	12649-12655	JJ	denotes	normal
T4758	12656-12657	-LRB-	denotes	(
T4759	12657-12664	NN	denotes	Slc26a4
T4760	12664-12665	SYM	denotes	+
T4761	12665-12666	HYPH	denotes	/
T4762	12666-12667	SYM	denotes	+
T4763	12667-12668	-RRB-	denotes	)
T4757	12669-12673	NNS	denotes	mice
T4764	12674-12679	VBG	denotes	using
T4765	12680-12682	DT	denotes	an
T4767	12683-12694	JJ	denotes	established
T4766	12695-12703	NN	denotes	antibody
T4768	12704-12705	-LRB-	denotes	[
T4769	12705-12706	CD	denotes	3
T4770	12706-12707	-RRB-	denotes	]
T4771	12707-12708	.	denotes	.
T4772	12708-12816	sentence	denotes	Staining was absent when the primary antibody was pre-absorbed with the antigenic peptide (data not shown).
T4773	12709-12717	NN	denotes	Staining
T4774	12718-12721	VBD	denotes	was
T4775	12722-12728	JJ	denotes	absent
T4776	12729-12733	WRB	denotes	when
T4778	12734-12737	DT	denotes	the
T4780	12738-12745	JJ	denotes	primary
T4779	12746-12754	NN	denotes	antibody
T4781	12755-12758	VBD	denotes	was
T4777	12759-12771	VBN	denotes	pre-absorbed
T4782	12772-12776	IN	denotes	with
T4783	12777-12780	DT	denotes	the
T4785	12781-12790	JJ	denotes	antigenic
T4784	12791-12798	NN	denotes	peptide
T4786	12799-12800	-LRB-	denotes	(
T4788	12800-12804	NNS	denotes	data
T4789	12805-12808	RB	denotes	not
T4787	12809-12814	VBN	denotes	shown
T4790	12814-12815	-RRB-	denotes	)
T4791	12815-12816	.	denotes	.
T4792	12816-13136	sentence	denotes	Strong expression of pendrin was observed not only in outer sulcus epithelial cells, as predicted from in situ hybridization data, but also in root cells, in apical membranes of spiral prominence surface epithelial cells and in apical membranes of spindle-shaped cells that are part of stria vascularis (Fig. 1a,1b,1c).
T4793	12817-12823	JJ	denotes	Strong
T4794	12824-12834	NN	denotes	expression
T4796	12835-12837	IN	denotes	of
T4797	12838-12845	NN	denotes	pendrin
T4798	12846-12849	VBD	denotes	was
T4795	12850-12858	VBN	denotes	observed
T4799	12859-12862	RB	denotes	not
T4801	12863-12867	RB	denotes	only
T4800	12868-12870	IN	denotes	in
T4802	12871-12876	JJ	denotes	outer
T4803	12877-12883	NN	denotes	sulcus
T4805	12884-12894	JJ	denotes	epithelial
T4804	12895-12900	NNS	denotes	cells
T4806	12900-12902	,	denotes	,
T4808	12902-12904	IN	denotes	as
T4807	12905-12914	VBN	denotes	predicted
T4809	12915-12919	IN	denotes	from
T4810	12920-12922	FW	denotes	in
T4811	12923-12927	FW	denotes	situ
T4813	12928-12941	NN	denotes	hybridization
T4812	12942-12946	NNS	denotes	data
T4814	12946-12948	,	denotes	,
T4815	12948-12951	CC	denotes	but
T4816	12952-12956	RB	denotes	also
T4817	12957-12959	IN	denotes	in
T4818	12960-12964	NN	denotes	root
T4819	12965-12970	NNS	denotes	cells
T4820	12970-12972	,	denotes	,
T4821	12972-12974	IN	denotes	in
T4822	12975-12981	JJ	denotes	apical
T4823	12982-12991	NNS	denotes	membranes
T4824	12992-12994	IN	denotes	of
T4825	12995-13001	JJ	denotes	spiral
T4826	13002-13012	NN	denotes	prominence
T4828	13013-13020	NN	denotes	surface
T4829	13021-13031	JJ	denotes	epithelial
T4827	13032-13037	NNS	denotes	cells
T4830	13038-13041	CC	denotes	and
T4831	13042-13044	IN	denotes	in
T4832	13045-13051	JJ	denotes	apical
T4833	13052-13061	NNS	denotes	membranes
T4834	13062-13064	IN	denotes	of
T4835	13065-13072	NN	denotes	spindle
T4837	13072-13073	HYPH	denotes	-
T4836	13073-13079	VBN	denotes	shaped
T4838	13080-13085	NNS	denotes	cells
T4839	13086-13090	WDT	denotes	that
T4840	13091-13094	VBP	denotes	are
T4841	13095-13099	NN	denotes	part
T4842	13100-13102	IN	denotes	of
T4843	13103-13108	NN	denotes	stria
T4844	13109-13119	NN	denotes	vascularis
T4845	13120-13121	-LRB-	denotes	(
T4847	13121-13125	NN	denotes	Fig.
T4848	13126-13128	CD	denotes	1a
T4849	13128-13129	,	denotes	,
T4850	13129-13131	CD	denotes	1b
T4851	13131-13132	,	denotes	,
T4846	13132-13134	CD	denotes	1c
T4852	13134-13135	-RRB-	denotes	)
T4853	13135-13136	.	denotes	.
T4854	13136-13241	sentence	denotes	The presence of pendrin in spindle-shaped cells suggests that these cells secrete HCO3 - into endolymph.
T4855	13137-13140	DT	denotes	The
T4856	13141-13149	NN	denotes	presence
T4858	13150-13152	IN	denotes	of
T4859	13153-13160	NN	denotes	pendrin
T4860	13161-13163	IN	denotes	in
T4861	13164-13171	NN	denotes	spindle
T4863	13171-13172	HYPH	denotes	-
T4862	13172-13178	VBN	denotes	shaped
T4864	13179-13184	NNS	denotes	cells
T4857	13185-13193	VBZ	denotes	suggests
T4865	13194-13198	IN	denotes	that
T4867	13199-13204	DT	denotes	these
T4868	13205-13210	NNS	denotes	cells
T4866	13211-13218	VBP	denotes	secrete
T4869	13219-13223	NN	denotes	HCO3
T4870	13224-13225	SYM	denotes	-
T4871	13226-13230	IN	denotes	into
T4872	13231-13240	NN	denotes	endolymph
T4873	13240-13241	.	denotes	.
T4874	13241-13320	sentence	denotes	Pendrin-mediated HCO3 - transport has previously been shown in the kidney [9].
T4875	13242-13249	NN	denotes	Pendrin
T4877	13249-13250	HYPH	denotes	-
T4876	13250-13258	VBN	denotes	mediated
T4879	13259-13263	NN	denotes	HCO3
T4880	13264-13265	SYM	denotes	-
T4878	13266-13275	NN	denotes	transport
T4882	13276-13279	VBZ	denotes	has
T4883	13280-13290	RB	denotes	previously
T4884	13291-13295	VBN	denotes	been
T4881	13296-13301	VBN	denotes	shown
T4885	13302-13304	IN	denotes	in
T4886	13305-13308	DT	denotes	the
T4887	13309-13315	NN	denotes	kidney
T4888	13316-13317	-LRB-	denotes	[
T4889	13317-13318	CD	denotes	9
T4890	13318-13319	-RRB-	denotes	]
T4891	13319-13320	.	denotes	.
T4892	13320-13436	sentence	denotes	HCO3 - in the cochlea may be generated from CO2 by carbonic anhydrase located in strial intermediate cells [18,19].
T4893	13321-13325	NN	denotes	HCO3
T4895	13326-13327	SYM	denotes	-
T4896	13328-13330	IN	denotes	in
T4897	13331-13334	DT	denotes	the
T4898	13335-13342	NN	denotes	cochlea
T4899	13343-13346	MD	denotes	may
T4900	13347-13349	VB	denotes	be
T4894	13350-13359	VBN	denotes	generated
T4901	13360-13364	IN	denotes	from
T4902	13365-13368	NN	denotes	CO2
T4903	13369-13371	IN	denotes	by
T4904	13372-13380	JJ	denotes	carbonic
T4905	13381-13390	NN	denotes	anhydrase
T4906	13391-13398	VBN	denotes	located
T4907	13399-13401	IN	denotes	in
T4908	13402-13408	JJ	denotes	strial
T4910	13409-13421	JJ	denotes	intermediate
T4909	13422-13427	NNS	denotes	cells
T4911	13428-13429	-LRB-	denotes	[
T4913	13429-13431	CD	denotes	18
T4914	13431-13432	,	denotes	,
T4912	13432-13434	CD	denotes	19
T4915	13434-13435	-RRB-	denotes	]
T4916	13435-13436	.	denotes	.
T4917	13436-13513	sentence	denotes	CO2 may be supplied by the metabolically highly active stria marginal cells.
T4918	13437-13440	NN	denotes	CO2
T4920	13441-13444	MD	denotes	may
T4921	13445-13447	VB	denotes	be
T4919	13448-13456	VBN	denotes	supplied
T4922	13457-13459	IN	denotes	by
T4923	13460-13463	DT	denotes	the
T4925	13464-13477	RB	denotes	metabolically
T4927	13478-13484	RB	denotes	highly
T4926	13485-13491	JJ	denotes	active
T4928	13492-13497	NN	denotes	stria
T4929	13498-13506	JJ	denotes	marginal
T4924	13507-13512	NNS	denotes	cells
T4930	13512-13513	.	denotes	.
T4931	13513-13644	sentence	denotes	It is conceivable that pendrin dysfunction interrupts HCO3 - secretion and leads to an accumulation of HCO3 - in stria vascularis.
T4932	13514-13516	PRP	denotes	It
T4933	13517-13519	VBZ	denotes	is
T4934	13520-13531	JJ	denotes	conceivable
T4935	13532-13536	IN	denotes	that
T4937	13537-13544	NN	denotes	pendrin
T4938	13545-13556	NN	denotes	dysfunction
T4936	13557-13567	VBZ	denotes	interrupts
T4939	13568-13572	NN	denotes	HCO3
T4941	13573-13574	SYM	denotes	-
T4940	13575-13584	NN	denotes	secretion
T4942	13585-13588	CC	denotes	and
T4943	13589-13594	VBZ	denotes	leads
T4944	13595-13597	IN	denotes	to
T4945	13598-13600	DT	denotes	an
T4946	13601-13613	NN	denotes	accumulation
T4947	13614-13616	IN	denotes	of
T4948	13617-13621	NN	denotes	HCO3
T4949	13622-13623	SYM	denotes	-
T4950	13624-13626	IN	denotes	in
T4951	13627-13632	NN	denotes	stria
T4952	13633-13643	NN	denotes	vascularis
T4953	13643-13644	.	denotes	.
T4954	13644-13756	sentence	denotes	Preliminary data (Wangemann et al., unpublished) support a role for pendrin in HCO3 - secretion into endolymph.
T4955	13645-13656	JJ	denotes	Preliminary
T4956	13657-13661	NNS	denotes	data
T4958	13662-13663	-LRB-	denotes	(
T4959	13663-13672	NNP	denotes	Wangemann
T4960	13673-13675	FW	denotes	et
T4961	13676-13679	FW	denotes	al.
T4962	13679-13681	,	denotes	,
T4963	13681-13692	JJ	denotes	unpublished
T4964	13692-13693	-RRB-	denotes	)
T4957	13694-13701	VBP	denotes	support
T4965	13702-13703	DT	denotes	a
T4966	13704-13708	NN	denotes	role
T4967	13709-13712	IN	denotes	for
T4968	13713-13720	NN	denotes	pendrin
T4969	13721-13723	IN	denotes	in
T4970	13724-13728	NN	denotes	HCO3
T4972	13729-13730	SYM	denotes	-
T4971	13731-13740	NN	denotes	secretion
T4973	13741-13745	IN	denotes	into
T4974	13746-13755	NN	denotes	endolymph
T4975	13755-13756	.	denotes	.
T4976	13756-13757	sentence	denotes
T7896	13767-13774	NN	denotes	Protein
T7897	13775-13787	NN	denotes	localization
T7898	13788-13790	IN	denotes	of
T7899	13791-13798	NN	denotes	pendrin
T7900	13798-13800	,	denotes	,
T7901	13800-13805	NN	denotes	KCNQ1
T7902	13805-13807	,	denotes	,
T7903	13807-13809	NN	denotes	ZO
T7904	13809-13810	HYPH	denotes	-
T7905	13810-13811	CD	denotes	1
T7906	13812-13815	CC	denotes	and
T7907	13816-13817	NN	denotes	F
T7908	13818-13823	NN	denotes	actin
T7909	13824-13826	IN	denotes	in
T7910	13827-13834	NN	denotes	cochlea
T7911	13835-13838	CC	denotes	and
T7912	13839-13849	JJ	denotes	vestibular
T7913	13850-13859	NN	denotes	labyrinth
T7914	13860-13862	IN	denotes	of
T7915	13863-13870	NN	denotes	Slc26a4
T7917	13870-13871	SYM	denotes	+
T7918	13871-13872	HYPH	denotes	/
T7919	13872-13873	SYM	denotes	+
T7920	13874-13877	CC	denotes	and
T7921	13878-13885	NN	denotes	Slc26a4
T7922	13885-13886	SYM	denotes	-
T7923	13886-13887	HYPH	denotes	/
T7924	13887-13888	SYM	denotes	-
T7916	13889-13893	NNS	denotes	mice
T7925	13893-13894	.	denotes	.
T7926	13894-13932	sentence	denotes	a: Overview of cochlea; bar = 100 μm.
T7927	13895-13896	LS	denotes	a
T7929	13896-13898	:	denotes	:
T7930	13898-13906	NN	denotes	Overview
T7931	13907-13909	IN	denotes	of
T7932	13910-13917	NN	denotes	cochlea
T7933	13917-13918	:	denotes	;
T7934	13919-13922	NN	denotes	bar
T7935	13923-13924	SYM	denotes	=
T7936	13925-13928	CD	denotes	100
T7928	13929-13931	NNS	denotes	μm
T7937	13931-13932	.	denotes	.
T7938	13932-13983	sentence	denotes	b-f: Detail of cochlear lateral wall; bar = 10 μm.
T7939	13933-13934	LS	denotes	b
T7941	13934-13935	HYPH	denotes	-
T7942	13935-13936	LS	denotes	f
T7943	13936-13938	:	denotes	:
T7944	13938-13944	NN	denotes	Detail
T7945	13945-13947	IN	denotes	of
T7946	13948-13956	JJ	denotes	cochlear
T7948	13957-13964	JJ	denotes	lateral
T7947	13965-13969	NN	denotes	wall
T7949	13969-13970	:	denotes	;
T7950	13971-13974	NN	denotes	bar
T7951	13975-13976	SYM	denotes	=
T7952	13977-13979	CD	denotes	10
T7940	13980-13982	NNS	denotes	μm
T7953	13982-13983	.	denotes	.
T7954	13983-14018	sentence	denotes	g: Detail of utricle; bar = 10 μm.
T7955	13984-13985	LS	denotes	g
T7957	13985-13987	:	denotes	:
T7958	13987-13993	NN	denotes	Detail
T7959	13994-13996	IN	denotes	of
T7960	13997-14004	NN	denotes	utricle
T7961	14004-14005	:	denotes	;
T7962	14006-14009	NN	denotes	bar
T7963	14010-14011	SYM	denotes	=
T7964	14012-14014	CD	denotes	10
T7956	14015-14017	NNS	denotes	μm
T7965	14017-14018	.	denotes	.
T7966	14018-14055	sentence	denotes	h-i: Detail of ampulla; bar = 10 μm.
T7967	14019-14020	LS	denotes	h
T7969	14020-14021	HYPH	denotes	-
T7970	14021-14022	LS	denotes	i
T7971	14022-14024	:	denotes	:
T7972	14024-14030	NN	denotes	Detail
T7973	14031-14033	IN	denotes	of
T7974	14034-14041	NN	denotes	ampulla
T7975	14041-14042	:	denotes	;
T7976	14043-14046	NN	denotes	bar
T7977	14047-14048	SYM	denotes	=
T7978	14049-14051	CD	denotes	10
T7968	14052-14054	NNS	denotes	μm
T7979	14054-14055	.	denotes	.
T7980	14055-14197	sentence	denotes	RM, Reissner's membrane; SC, spindle-shaped cells, SMC, strial marginal cells; SV, stria vascularis; SL, spiral ligament; LIM, spiral limbus.
T7981	14056-14058	NN	denotes	RM
T7982	14058-14060	,	denotes	,
T7983	14060-14068	NNP	denotes	Reissner
T7985	14068-14070	POS	denotes	's
T7984	14071-14079	NN	denotes	membrane
T7986	14079-14080	,	denotes	;
T7987	14081-14083	NN	denotes	SC
T7988	14083-14085	,	denotes	,
T7989	14085-14092	NN	denotes	spindle
T7991	14092-14093	HYPH	denotes	-
T7990	14093-14099	VBN	denotes	shaped
T7992	14100-14105	NNS	denotes	cells
T7993	14105-14107	,	denotes	,
T7994	14107-14110	NN	denotes	SMC
T7995	14110-14112	,	denotes	,
T7996	14112-14118	JJ	denotes	strial
T7997	14119-14127	JJ	denotes	marginal
T7998	14128-14133	NNS	denotes	cells
T7999	14133-14134	,	denotes	;
T8000	14135-14137	NN	denotes	SV
T8001	14137-14139	,	denotes	,
T8002	14139-14144	NN	denotes	stria
T8003	14145-14155	NN	denotes	vascularis
T8004	14155-14156	,	denotes	;
T8005	14157-14159	NN	denotes	SL
T8006	14159-14161	,	denotes	,
T8007	14161-14167	JJ	denotes	spiral
T8008	14168-14176	NN	denotes	ligament
T8009	14176-14177	,	denotes	;
T8010	14178-14181	NN	denotes	LIM
T8011	14181-14183	,	denotes	,
T8012	14183-14189	JJ	denotes	spiral
T8013	14190-14196	NN	denotes	limbus
T8014	14196-14197	.	denotes	.
T8015	14197-14511	sentence	denotes	BC, basal cells; SP, spiral prominence epithelial cells; RC, root cells; OS, outer sulcus epithelial cells; VHC, vestibular hair cells; VTC, vestibular transitional cells; VDC, vestibular dark cells; arrows, basal cells at the top and bottom of stria vascularis form tight junctions with surface epithelial cells.
T8016	14198-14200	NN	denotes	BC
T8017	14200-14202	,	denotes	,
T8018	14202-14207	JJ	denotes	basal
T8019	14208-14213	NNS	denotes	cells
T8020	14213-14214	,	denotes	;
T8021	14215-14217	NN	denotes	SP
T8022	14217-14219	,	denotes	,
T8023	14219-14225	JJ	denotes	spiral
T8024	14226-14236	NN	denotes	prominence
T8026	14237-14247	JJ	denotes	epithelial
T8025	14248-14253	NNS	denotes	cells
T8027	14253-14254	,	denotes	;
T8028	14255-14257	NN	denotes	RC
T8029	14257-14259	,	denotes	,
T8030	14259-14263	NN	denotes	root
T8031	14264-14269	NNS	denotes	cells
T8032	14269-14270	,	denotes	;
T8033	14271-14273	NN	denotes	OS
T8034	14273-14275	,	denotes	,
T8035	14275-14280	JJ	denotes	outer
T8036	14281-14287	NN	denotes	sulcus
T8038	14288-14298	JJ	denotes	epithelial
T8037	14299-14304	NNS	denotes	cells
T8039	14304-14305	,	denotes	;
T8040	14306-14309	NN	denotes	VHC
T8041	14309-14311	,	denotes	,
T8042	14311-14321	JJ	denotes	vestibular
T8044	14322-14326	NN	denotes	hair
T8043	14327-14332	NNS	denotes	cells
T8045	14332-14333	,	denotes	;
T8046	14334-14337	NN	denotes	VTC
T8047	14337-14339	,	denotes	,
T8048	14339-14349	JJ	denotes	vestibular
T8050	14350-14362	JJ	denotes	transitional
T8049	14363-14368	NNS	denotes	cells
T8051	14368-14369	,	denotes	;
T8052	14370-14373	NN	denotes	VDC
T8053	14373-14375	,	denotes	,
T8054	14375-14385	JJ	denotes	vestibular
T8056	14386-14390	JJ	denotes	dark
T8055	14391-14396	NNS	denotes	cells
T8057	14396-14397	,	denotes	;
T8058	14398-14404	NNS	denotes	arrows
T8060	14404-14406	,	denotes	,
T8061	14406-14411	JJ	denotes	basal
T8062	14412-14417	NNS	denotes	cells
T8063	14418-14420	IN	denotes	at
T8064	14421-14424	DT	denotes	the
T8065	14425-14428	NN	denotes	top
T8066	14429-14432	CC	denotes	and
T8067	14433-14439	NN	denotes	bottom
T8068	14440-14442	IN	denotes	of
T8069	14443-14448	NN	denotes	stria
T8070	14449-14459	NN	denotes	vascularis
T8059	14460-14464	VBP	denotes	form
T8071	14465-14470	JJ	denotes	tight
T8072	14471-14480	NNS	denotes	junctions
T8073	14481-14485	IN	denotes	with
T8074	14486-14493	NN	denotes	surface
T8076	14494-14504	JJ	denotes	epithelial
T8075	14505-14510	NNS	denotes	cells
T8077	14510-14511	.	denotes	.
T4978	14512-14513	NN	denotes	T
T4977	14512-14517	sentence	denotes	The e
T4979	14513-14515	DT	denotes	he
T4980	14516-14517	NN	denotes	e
T4982	14517-14522	NN	denotes	xtent
T4981	14517-14801	sentence	denotes	xtent of pendrin expression in spiral prominence cells and stria vascularis was determined by labeling KCNQ1, a K+ channel that is expressed in strial marginal cells, and ZO-1, a tight junction protein that labels basal cells and thereby delineates the boundaries of stria vascularis.
T4984	14523-14525	IN	denotes	of
T4985	14526-14533	NN	denotes	pendrin
T4986	14534-14544	NN	denotes	expression
T4987	14545-14547	IN	denotes	in
T4988	14548-14554	JJ	denotes	spiral
T4990	14555-14565	NN	denotes	prominence
T4989	14566-14571	NNS	denotes	cells
T4991	14572-14575	CC	denotes	and
T4992	14576-14581	NN	denotes	stria
T4993	14582-14592	NN	denotes	vascularis
T4994	14593-14596	VBD	denotes	was
T4983	14597-14607	VBN	denotes	determined
T4995	14608-14610	IN	denotes	by
T4996	14611-14619	VBG	denotes	labeling
T4997	14620-14625	NN	denotes	KCNQ1
T4998	14625-14627	,	denotes	,
T4999	14627-14628	DT	denotes	a
T5001	14629-14630	NN	denotes	K
T5002	14630-14631	SYM	denotes	+
T5000	14632-14639	NN	denotes	channel
T5003	14640-14644	WDT	denotes	that
T5005	14645-14647	VBZ	denotes	is
T5004	14648-14657	VBN	denotes	expressed
T5006	14658-14660	IN	denotes	in
T5007	14661-14667	JJ	denotes	strial
T5008	14668-14676	JJ	denotes	marginal
T5009	14677-14682	NNS	denotes	cells
T5010	14682-14684	,	denotes	,
T5011	14684-14687	CC	denotes	and
T5012	14688-14690	NN	denotes	ZO
T5013	14690-14691	HYPH	denotes	-
T5014	14691-14692	CD	denotes	1
T5015	14692-14694	,	denotes	,
T5016	14694-14695	DT	denotes	a
T5018	14696-14701	JJ	denotes	tight
T5019	14702-14710	NN	denotes	junction
T5017	14711-14718	NN	denotes	protein
T5020	14719-14723	WDT	denotes	that
T5021	14724-14730	VBZ	denotes	labels
T5022	14731-14736	JJ	denotes	basal
T5023	14737-14742	NNS	denotes	cells
T5024	14743-14746	CC	denotes	and
T5025	14747-14754	RB	denotes	thereby
T5026	14755-14765	VBZ	denotes	delineates
T5027	14766-14769	DT	denotes	the
T5028	14770-14780	NNS	denotes	boundaries
T5029	14781-14783	IN	denotes	of
T5030	14784-14789	NN	denotes	stria
T5031	14790-14800	NN	denotes	vascularis
T5032	14800-14801	.	denotes	.
T5033	14801-15062	sentence	denotes	Dual labeling experiments demonstrated that pendrin is expressed in spindle-shaped cells, which are surface epithelial cells in stria vascularis adjacent to strial marginal cells near the borders of both the spiral prominence and Reissner's membrane (Fig. 1c).
T5034	14802-14806	JJ	denotes	Dual
T5036	14807-14815	NN	denotes	labeling
T5035	14816-14827	NNS	denotes	experiments
T5037	14828-14840	VBD	denotes	demonstrated
T5038	14841-14845	IN	denotes	that
T5040	14846-14853	NN	denotes	pendrin
T5041	14854-14856	VBZ	denotes	is
T5039	14857-14866	VBN	denotes	expressed
T5042	14867-14869	IN	denotes	in
T5043	14870-14877	NN	denotes	spindle
T5045	14877-14878	HYPH	denotes	-
T5044	14878-14884	VBN	denotes	shaped
T5046	14885-14890	NNS	denotes	cells
T5047	14890-14892	,	denotes	,
T5048	14892-14897	WDT	denotes	which
T5049	14898-14901	VBP	denotes	are
T5050	14902-14909	JJ	denotes	surface
T5052	14910-14920	JJ	denotes	epithelial
T5051	14921-14926	NNS	denotes	cells
T5053	14927-14929	IN	denotes	in
T5054	14930-14935	NN	denotes	stria
T5055	14936-14946	NN	denotes	vascularis
T5056	14947-14955	JJ	denotes	adjacent
T5057	14956-14958	IN	denotes	to
T5058	14959-14965	JJ	denotes	strial
T5060	14966-14974	JJ	denotes	marginal
T5059	14975-14980	NNS	denotes	cells
T5061	14981-14985	IN	denotes	near
T5062	14986-14989	DT	denotes	the
T5063	14990-14997	NNS	denotes	borders
T5064	14998-15000	IN	denotes	of
T5065	15001-15005	CC	denotes	both
T5067	15006-15009	DT	denotes	the
T5068	15010-15016	JJ	denotes	spiral
T5066	15017-15027	NN	denotes	prominence
T5069	15028-15031	CC	denotes	and
T5070	15032-15040	NNP	denotes	Reissner
T5072	15040-15042	POS	denotes	's
T5071	15043-15051	NN	denotes	membrane
T5073	15052-15053	-LRB-	denotes	(
T5074	15053-15057	NN	denotes	Fig.
T5075	15058-15060	CD	denotes	1c
T5076	15060-15061	-RRB-	denotes	)
T5077	15061-15062	.	denotes	.
T5078	15062-15179	sentence	denotes	In situ hybridization in the vestibular labyrinth suggested that pendrin mRNA is expressed in non-sensory cells [8].
T5079	15063-15065	FW	denotes	In
T5080	15066-15070	FW	denotes	situ
T5081	15071-15084	NN	denotes	hybridization
T5083	15085-15087	IN	denotes	in
T5084	15088-15091	DT	denotes	the
T5086	15092-15102	JJ	denotes	vestibular
T5085	15103-15112	NN	denotes	labyrinth
T5082	15113-15122	VBD	denotes	suggested
T5087	15123-15127	IN	denotes	that
T5089	15128-15135	NN	denotes	pendrin
T5090	15136-15140	NN	denotes	mRNA
T5091	15141-15143	VBZ	denotes	is
T5088	15144-15153	VBN	denotes	expressed
T5092	15154-15156	IN	denotes	in
T5093	15157-15168	JJ	denotes	non-sensory
T5094	15169-15174	NNS	denotes	cells
T5095	15175-15176	-LRB-	denotes	[
T5096	15176-15177	CD	denotes	8
T5097	15177-15178	-RRB-	denotes	]
T5098	15178-15179	.	denotes	.
T5099	15179-15385	sentence	denotes	Using confocal immunocytochemistry on cryosections, we observed strong expression of the pendrin protein in the apical membrane of vestibular transitional cells in the utricle and ampullae (Fig. 1g,1h,1i).
T5100	15180-15185	VBG	denotes	Using
T5102	15186-15194	JJ	denotes	confocal
T5103	15195-15214	NN	denotes	immunocytochemistry
T5104	15215-15217	IN	denotes	on
T5105	15218-15230	NNS	denotes	cryosections
T5106	15230-15232	,	denotes	,
T5107	15232-15234	PRP	denotes	we
T5101	15235-15243	VBD	denotes	observed
T5108	15244-15250	JJ	denotes	strong
T5109	15251-15261	NN	denotes	expression
T5110	15262-15264	IN	denotes	of
T5111	15265-15268	DT	denotes	the
T5113	15269-15276	NN	denotes	pendrin
T5112	15277-15284	NN	denotes	protein
T5114	15285-15287	IN	denotes	in
T5115	15288-15291	DT	denotes	the
T5117	15292-15298	JJ	denotes	apical
T5116	15299-15307	NN	denotes	membrane
T5118	15308-15310	IN	denotes	of
T5119	15311-15321	JJ	denotes	vestibular
T5121	15322-15334	JJ	denotes	transitional
T5120	15335-15340	NNS	denotes	cells
T5122	15341-15343	IN	denotes	in
T5123	15344-15347	DT	denotes	the
T5124	15348-15355	NN	denotes	utricle
T5125	15356-15359	CC	denotes	and
T5126	15360-15368	NNS	denotes	ampullae
T5127	15369-15370	-LRB-	denotes	(
T5129	15370-15374	NN	denotes	Fig.
T5130	15375-15377	CD	denotes	1g
T5131	15377-15378	,	denotes	,
T5132	15378-15380	CD	denotes	1h
T5133	15380-15381	,	denotes	,
T5128	15381-15383	CD	denotes	1i
T5134	15383-15384	-RRB-	denotes	)
T5135	15384-15385	.	denotes	.
T5136	15385-15671	sentence	denotes	Dual labeling with KCNQ1 demonstrated that pendrin expression was clearly limited to vestibular transitional cells and did not extend to other non-sensory cells such as vestibular dark cells, which were clearly identified by the expression of KCNQ1 and KCNE1 in their apical membranes.
T5137	15386-15390	JJ	denotes	Dual
T5138	15391-15399	NN	denotes	labeling
T5140	15400-15404	IN	denotes	with
T5141	15405-15410	NN	denotes	KCNQ1
T5139	15411-15423	VBD	denotes	demonstrated
T5142	15424-15428	IN	denotes	that
T5144	15429-15436	NN	denotes	pendrin
T5145	15437-15447	NN	denotes	expression
T5146	15448-15451	VBD	denotes	was
T5147	15452-15459	RB	denotes	clearly
T5143	15460-15467	VBN	denotes	limited
T5148	15468-15470	IN	denotes	to
T5149	15471-15481	JJ	denotes	vestibular
T5151	15482-15494	JJ	denotes	transitional
T5150	15495-15500	NNS	denotes	cells
T5152	15501-15504	CC	denotes	and
T5153	15505-15508	VBD	denotes	did
T5155	15509-15512	RB	denotes	not
T5154	15513-15519	VB	denotes	extend
T5156	15520-15522	IN	denotes	to
T5157	15523-15528	JJ	denotes	other
T5159	15529-15540	JJ	denotes	non-sensory
T5158	15541-15546	NNS	denotes	cells
T5160	15547-15551	JJ	denotes	such
T5161	15552-15554	IN	denotes	as
T5162	15555-15565	JJ	denotes	vestibular
T5164	15566-15570	JJ	denotes	dark
T5163	15571-15576	NNS	denotes	cells
T5165	15576-15578	,	denotes	,
T5166	15578-15583	WDT	denotes	which
T5168	15584-15588	VBD	denotes	were
T5169	15589-15596	RB	denotes	clearly
T5167	15597-15607	VBN	denotes	identified
T5170	15608-15610	IN	denotes	by
T5171	15611-15614	DT	denotes	the
T5172	15615-15625	NN	denotes	expression
T5173	15626-15628	IN	denotes	of
T5174	15629-15634	NN	denotes	KCNQ1
T5175	15635-15638	CC	denotes	and
T5176	15639-15644	NN	denotes	KCNE1
T5177	15645-15647	IN	denotes	in
T5178	15648-15653	PRP$	denotes	their
T5180	15654-15660	JJ	denotes	apical
T5179	15661-15670	NNS	denotes	membranes
T5181	15670-15671	.	denotes	.
T5182	15671-15799	sentence	denotes	The onset of pendrin expression during development of the mouse inner ear has been determined to be embryonic day (ED) 13 [14].
T5183	15672-15675	DT	denotes	The
T5184	15676-15681	NN	denotes	onset
T5186	15682-15684	IN	denotes	of
T5187	15685-15692	NN	denotes	pendrin
T5188	15693-15703	NN	denotes	expression
T5189	15704-15710	IN	denotes	during
T5190	15711-15722	NN	denotes	development
T5191	15723-15725	IN	denotes	of
T5192	15726-15729	DT	denotes	the
T5194	15730-15735	NN	denotes	mouse
T5195	15736-15741	JJ	denotes	inner
T5193	15742-15745	NN	denotes	ear
T5196	15746-15749	VBZ	denotes	has
T5197	15750-15754	VBN	denotes	been
T5185	15755-15765	VBN	denotes	determined
T5198	15766-15768	TO	denotes	to
T5199	15769-15771	VB	denotes	be
T5200	15772-15781	JJ	denotes	embryonic
T5201	15782-15785	NN	denotes	day
T5202	15786-15787	-LRB-	denotes	(
T5203	15787-15789	NN	denotes	ED
T5204	15789-15790	-RRB-	denotes	)
T5205	15791-15793	CD	denotes	13
T5206	15794-15795	-LRB-	denotes	[
T5207	15795-15797	CD	denotes	14
T5208	15797-15798	-RRB-	denotes	]
T5209	15798-15799	.	denotes	.
T5210	15799-16029	sentence	denotes	Morphologically detectable differences in the inner ears of Slc26a4+/+ and Slc26a4-/- mice become evident as early as ED 15, when Slc26a4-/- mice start to develop an enlarged endolymphatic space that persists into adulthood [14].
T5211	15800-15815	RB	denotes	Morphologically
T5212	15816-15826	JJ	denotes	detectable
T5213	15827-15838	NNS	denotes	differences
T5215	15839-15841	IN	denotes	in
T5216	15842-15845	DT	denotes	the
T5218	15846-15851	JJ	denotes	inner
T5217	15852-15856	NNS	denotes	ears
T5219	15857-15859	IN	denotes	of
T5220	15860-15867	NN	denotes	Slc26a4
T5222	15867-15868	SYM	denotes	+
T5223	15868-15869	HYPH	denotes	/
T5224	15869-15870	SYM	denotes	+
T5225	15871-15874	CC	denotes	and
T5226	15875-15882	NN	denotes	Slc26a4
T5227	15882-15883	SYM	denotes	-
T5228	15883-15884	HYPH	denotes	/
T5229	15884-15885	SYM	denotes	-
T5221	15886-15890	NNS	denotes	mice
T5214	15891-15897	VBP	denotes	become
T5230	15898-15905	JJ	denotes	evident
T5231	15906-15908	RB	denotes	as
T5232	15909-15914	RB	denotes	early
T5233	15915-15917	IN	denotes	as
T5234	15918-15920	NN	denotes	ED
T5235	15921-15923	CD	denotes	15
T5236	15923-15925	,	denotes	,
T5237	15925-15929	WRB	denotes	when
T5239	15930-15937	NN	denotes	Slc26a4
T5241	15937-15938	SYM	denotes	-
T5242	15938-15939	HYPH	denotes	/
T5243	15939-15940	SYM	denotes	-
T5240	15941-15945	NNS	denotes	mice
T5238	15946-15951	VBP	denotes	start
T5244	15952-15954	TO	denotes	to
T5245	15955-15962	VB	denotes	develop
T5246	15963-15965	DT	denotes	an
T5248	15966-15974	JJ	denotes	enlarged
T5249	15975-15988	JJ	denotes	endolymphatic
T5247	15989-15994	NN	denotes	space
T5250	15995-15999	WDT	denotes	that
T5251	16000-16008	VBZ	denotes	persists
T5252	16009-16013	IN	denotes	into
T5253	16014-16023	NN	denotes	adulthood
T5254	16024-16025	-LRB-	denotes	[
T5255	16025-16027	CD	denotes	14
T5256	16027-16028	-RRB-	denotes	]
T5257	16028-16029	.	denotes	.
T5258	16029-16174	sentence	denotes	Interestingly, sensory hair cells in the cochlea appear normal until postnatal day (PD) 7 but show clear evidence of degeneration by PD 15 [14].
T5259	16030-16043	RB	denotes	Interestingly
T5261	16043-16045	,	denotes	,
T5262	16045-16052	JJ	denotes	sensory
T5264	16053-16057	NN	denotes	hair
T5263	16058-16063	NNS	denotes	cells
T5265	16064-16066	IN	denotes	in
T5266	16067-16070	DT	denotes	the
T5267	16071-16078	NN	denotes	cochlea
T5260	16079-16085	VBP	denotes	appear
T5268	16086-16092	JJ	denotes	normal
T5269	16093-16098	IN	denotes	until
T5270	16099-16108	JJ	denotes	postnatal
T5271	16109-16112	NN	denotes	day
T5272	16113-16114	-LRB-	denotes	(
T5273	16114-16116	NN	denotes	PD
T5274	16116-16117	-RRB-	denotes	)
T5275	16118-16119	CD	denotes	7
T5276	16120-16123	CC	denotes	but
T5277	16124-16128	VBP	denotes	show
T5278	16129-16134	JJ	denotes	clear
T5279	16135-16143	NN	denotes	evidence
T5280	16144-16146	IN	denotes	of
T5281	16147-16159	NN	denotes	degeneration
T5282	16160-16162	IN	denotes	by
T5283	16163-16165	NN	denotes	PD
T5284	16166-16168	CD	denotes	15
T5285	16169-16170	-LRB-	denotes	[
T5286	16170-16172	CD	denotes	14
T5287	16172-16173	-RRB-	denotes	]
T5288	16173-16174	.	denotes	.
T5289	16174-16330	sentence	denotes	These observations suggest that the cochlear environment supports the survival of sensory hair cells in spite of the enlargement of the endolymphatic duct.
T5290	16175-16180	DT	denotes	These
T5291	16181-16193	NNS	denotes	observations
T5292	16194-16201	VBP	denotes	suggest
T5293	16202-16206	IN	denotes	that
T5295	16207-16210	DT	denotes	the
T5297	16211-16219	JJ	denotes	cochlear
T5296	16220-16231	NN	denotes	environment
T5294	16232-16240	VBZ	denotes	supports
T5298	16241-16244	DT	denotes	the
T5299	16245-16253	NN	denotes	survival
T5300	16254-16256	IN	denotes	of
T5301	16257-16264	JJ	denotes	sensory
T5303	16265-16269	NN	denotes	hair
T5302	16270-16275	NNS	denotes	cells
T5304	16276-16278	IN	denotes	in
T5305	16279-16284	NN	denotes	spite
T5306	16285-16287	IN	denotes	of
T5307	16288-16291	DT	denotes	the
T5308	16292-16303	NN	denotes	enlargement
T5309	16304-16306	IN	denotes	of
T5310	16307-16310	DT	denotes	the
T5312	16311-16324	JJ	denotes	endolymphatic
T5311	16325-16329	NN	denotes	duct
T5313	16329-16330	.	denotes	.
T5314	16330-16497	sentence	denotes	A normal endolymphatic K+ concentration, which is critical for hair cell survival [20], is established at PD 3 [21] and may persist in Slc26a4-/- at least until PD 7.
T5315	16331-16332	DT	denotes	A
T5317	16333-16339	JJ	denotes	normal
T5318	16340-16353	JJ	denotes	endolymphatic
T5319	16354-16355	NN	denotes	K
T5320	16355-16356	SYM	denotes	+
T5316	16357-16370	NN	denotes	concentration
T5322	16370-16372	,	denotes	,
T5323	16372-16377	WDT	denotes	which
T5324	16378-16380	VBZ	denotes	is
T5325	16381-16389	JJ	denotes	critical
T5326	16390-16393	IN	denotes	for
T5327	16394-16398	NN	denotes	hair
T5328	16399-16403	NN	denotes	cell
T5329	16404-16412	NN	denotes	survival
T5330	16413-16414	-LRB-	denotes	[
T5331	16414-16416	CD	denotes	20
T5332	16416-16417	-RRB-	denotes	]
T5333	16417-16419	,	denotes	,
T5334	16419-16421	VBZ	denotes	is
T5321	16422-16433	VBN	denotes	established
T5335	16434-16436	IN	denotes	at
T5336	16437-16439	NN	denotes	PD
T5337	16440-16441	CD	denotes	3
T5338	16442-16443	-LRB-	denotes	[
T5339	16443-16445	CD	denotes	21
T5340	16445-16446	-RRB-	denotes	]
T5341	16447-16450	CC	denotes	and
T5342	16451-16454	MD	denotes	may
T5343	16455-16462	VB	denotes	persist
T5344	16463-16465	IN	denotes	in
T5345	16466-16473	NN	denotes	Slc26a4
T5346	16473-16474	SYM	denotes	-
T5347	16474-16475	HYPH	denotes	/
T5348	16475-16476	SYM	denotes	-
T5349	16477-16479	RB	denotes	at
T5350	16480-16485	RBS	denotes	least
T5351	16486-16491	IN	denotes	until
T5352	16492-16494	NN	denotes	PD
T5353	16495-16496	CD	denotes	7
T5354	16496-16497	.	denotes	.
T5355	16497-16616	sentence	denotes	The time period between PD 7 and 15 is the time when the endocochlear potential develops at the onset of hearing [22].
T5356	16498-16501	DT	denotes	The
T5358	16502-16506	NN	denotes	time
T5357	16507-16513	NN	denotes	period
T5360	16514-16521	IN	denotes	between
T5361	16522-16524	NN	denotes	PD
T5362	16525-16526	CD	denotes	7
T5363	16527-16530	CC	denotes	and
T5364	16531-16533	CD	denotes	15
T5359	16534-16536	VBZ	denotes	is
T5365	16537-16540	DT	denotes	the
T5366	16541-16545	NN	denotes	time
T5367	16546-16550	WRB	denotes	when
T5369	16551-16554	DT	denotes	the
T5371	16555-16567	JJ	denotes	endocochlear
T5370	16568-16577	NN	denotes	potential
T5368	16578-16586	VBZ	denotes	develops
T5372	16587-16589	IN	denotes	at
T5373	16590-16593	DT	denotes	the
T5374	16594-16599	NN	denotes	onset
T5375	16600-16602	IN	denotes	of
T5376	16603-16610	NN	denotes	hearing
T5377	16611-16612	-LRB-	denotes	[
T5378	16612-16614	CD	denotes	22
T5379	16614-16615	-RRB-	denotes	]
T5380	16615-16616	.	denotes	.
T5381	16616-16781	sentence	denotes	We hypothesized that a lack of a normal endocochlear potential or an alteration of the endolymphatic K+ concentration could account for deafness in Slc26a4-/- mice.
T5382	16617-16619	PRP	denotes	We
T5383	16620-16632	VBD	denotes	hypothesized
T5384	16633-16637	IN	denotes	that
T5386	16638-16639	DT	denotes	a
T5387	16640-16644	NN	denotes	lack
T5388	16645-16647	IN	denotes	of
T5389	16648-16649	DT	denotes	a
T5391	16650-16656	JJ	denotes	normal
T5392	16657-16669	JJ	denotes	endocochlear
T5390	16670-16679	NN	denotes	potential
T5393	16680-16682	CC	denotes	or
T5394	16683-16685	DT	denotes	an
T5395	16686-16696	NN	denotes	alteration
T5396	16697-16699	IN	denotes	of
T5397	16700-16703	DT	denotes	the
T5399	16704-16717	JJ	denotes	endolymphatic
T5400	16718-16719	NN	denotes	K
T5401	16719-16720	SYM	denotes	+
T5398	16721-16734	NN	denotes	concentration
T5402	16735-16740	MD	denotes	could
T5385	16741-16748	VB	denotes	account
T5403	16749-16752	IN	denotes	for
T5404	16753-16761	NN	denotes	deafness
T5405	16762-16764	IN	denotes	in
T5406	16765-16772	NN	denotes	Slc26a4
T5408	16772-16773	SYM	denotes	-
T5409	16773-16774	HYPH	denotes	/
T5410	16774-16775	SYM	denotes	-
T5407	16776-16780	NNS	denotes	mice
T5411	16780-16781	.	denotes	.
T5412	16781-16932	sentence	denotes	Measurements revealed that the endocochlear potential was absent but that the endolymphatic K+ concentration was normal in adult Slc26a4-/- (Fig. 2a).
T5413	16782-16794	NNS	denotes	Measurements
T5414	16795-16803	VBD	denotes	revealed
T5415	16804-16808	IN	denotes	that
T5417	16809-16812	DT	denotes	the
T5419	16813-16825	JJ	denotes	endocochlear
T5418	16826-16835	NN	denotes	potential
T5416	16836-16839	VBD	denotes	was
T5420	16840-16846	JJ	denotes	absent
T5421	16847-16850	CC	denotes	but
T5422	16851-16855	IN	denotes	that
T5424	16856-16859	DT	denotes	the
T5426	16860-16873	JJ	denotes	endolymphatic
T5427	16874-16875	NN	denotes	K
T5428	16875-16876	SYM	denotes	+
T5425	16877-16890	NN	denotes	concentration
T5423	16891-16894	VBD	denotes	was
T5429	16895-16901	JJ	denotes	normal
T5430	16902-16904	IN	denotes	in
T5431	16905-16910	JJ	denotes	adult
T5432	16911-16918	NN	denotes	Slc26a4
T5433	16918-16919	SYM	denotes	-
T5434	16919-16920	HYPH	denotes	/
T5435	16920-16921	SYM	denotes	-
T5436	16922-16923	-LRB-	denotes	(
T5437	16923-16927	NN	denotes	Fig.
T5438	16928-16930	CD	denotes	2a
T5439	16930-16931	-RRB-	denotes	)
T5440	16931-16932	.	denotes	.
T5441	16932-17132	sentence	denotes	No significant differences between Slc26a4+/+ and Slc26a4-/- mice were found in perilymphatic (Fig. 2a) or plasma K+ concentrations (Slc26a4+/+, 4.9 ± 0.3 mM, n = 6; Slc26a4-/-, 5.1 ± 0.3 mM, n = 6).
T5442	16933-16935	DT	denotes	No
T5444	16936-16947	JJ	denotes	significant
T5443	16948-16959	NNS	denotes	differences
T5446	16960-16967	IN	denotes	between
T5447	16968-16975	NN	denotes	Slc26a4
T5449	16975-16976	SYM	denotes	+
T5450	16976-16977	HYPH	denotes	/
T5451	16977-16978	SYM	denotes	+
T5452	16979-16982	CC	denotes	and
T5453	16983-16990	NN	denotes	Slc26a4
T5454	16990-16991	SYM	denotes	-
T5455	16991-16992	HYPH	denotes	/
T5456	16992-16993	SYM	denotes	-
T5448	16994-16998	NNS	denotes	mice
T5457	16999-17003	VBD	denotes	were
T5445	17004-17009	VBN	denotes	found
T5458	17010-17012	IN	denotes	in
T5459	17013-17026	JJ	denotes	perilymphatic
T5461	17027-17028	-LRB-	denotes	(
T5462	17028-17032	NN	denotes	Fig.
T5463	17033-17035	CD	denotes	2a
T5464	17035-17036	-RRB-	denotes	)
T5465	17037-17039	CC	denotes	or
T5466	17040-17046	NN	denotes	plasma
T5467	17047-17048	NN	denotes	K
T5468	17048-17049	SYM	denotes	+
T5460	17050-17064	NNS	denotes	concentrations
T5469	17065-17066	-LRB-	denotes	(
T5471	17066-17073	NN	denotes	Slc26a4
T5473	17073-17074	SYM	denotes	+
T5474	17074-17075	HYPH	denotes	/
T5475	17075-17076	SYM	denotes	+
T5476	17076-17078	,	denotes	,
T5477	17078-17081	CD	denotes	4.9
T5479	17082-17083	SYM	denotes	±
T5478	17084-17087	CD	denotes	0.3
T5480	17088-17090	NNS	denotes	mM
T5481	17090-17092	,	denotes	,
T5482	17092-17093	NN	denotes	n
T5483	17094-17095	SYM	denotes	=
T5472	17096-17097	CD	denotes	6
T5484	17097-17098	:	denotes	;
T5485	17099-17106	NN	denotes	Slc26a4
T5486	17106-17107	SYM	denotes	-
T5487	17107-17108	HYPH	denotes	/
T5488	17108-17109	SYM	denotes	-
T5489	17109-17111	,	denotes	,
T5490	17111-17114	CD	denotes	5.1
T5492	17115-17116	SYM	denotes	±
T5491	17117-17120	CD	denotes	0.3
T5493	17121-17123	NNS	denotes	mM
T5494	17123-17125	,	denotes	,
T5495	17125-17126	NN	denotes	n
T5496	17127-17128	SYM	denotes	=
T5470	17129-17130	CD	denotes	6
T5497	17130-17131	-RRB-	denotes	)
T5498	17131-17132	.	denotes	.
T5499	17132-17324	sentence	denotes	These observations suggest that a primary event leading to deafness in Slc26a4-/- mice, and potentially in patients suffering from Pendred syndrome, is the loss of the endocochlear potential.
T5500	17133-17138	DT	denotes	These
T5501	17139-17151	NNS	denotes	observations
T5502	17152-17159	VBP	denotes	suggest
T5503	17160-17164	IN	denotes	that
T5505	17165-17166	DT	denotes	a
T5507	17167-17174	JJ	denotes	primary
T5506	17175-17180	NN	denotes	event
T5508	17181-17188	VBG	denotes	leading
T5509	17189-17191	IN	denotes	to
T5510	17192-17200	NN	denotes	deafness
T5511	17201-17203	IN	denotes	in
T5512	17204-17211	NN	denotes	Slc26a4
T5514	17211-17212	SYM	denotes	-
T5515	17212-17213	HYPH	denotes	/
T5516	17213-17214	SYM	denotes	-
T5513	17215-17219	NNS	denotes	mice
T5517	17219-17221	,	denotes	,
T5518	17221-17224	CC	denotes	and
T5519	17225-17236	RB	denotes	potentially
T5520	17237-17239	IN	denotes	in
T5521	17240-17248	NNS	denotes	patients
T5522	17249-17258	VBG	denotes	suffering
T5523	17259-17263	IN	denotes	from
T5524	17264-17271	NNP	denotes	Pendred
T5525	17272-17280	NN	denotes	syndrome
T5526	17280-17282	,	denotes	,
T5504	17282-17284	VBZ	denotes	is
T5527	17285-17288	DT	denotes	the
T5528	17289-17293	NN	denotes	loss
T5529	17294-17296	IN	denotes	of
T5530	17297-17300	DT	denotes	the
T5532	17301-17313	JJ	denotes	endocochlear
T5531	17314-17323	NN	denotes	potential
T5533	17323-17324	.	denotes	.
T5534	17324-17417	sentence	denotes	Degeneration of hair cells is probably a response to the loss of the endocochlear potential.
T5535	17325-17337	NN	denotes	Degeneration
T5537	17338-17340	IN	denotes	of
T5538	17341-17345	NN	denotes	hair
T5539	17346-17351	NNS	denotes	cells
T5536	17352-17354	VBZ	denotes	is
T5540	17355-17363	RB	denotes	probably
T5541	17364-17365	DT	denotes	a
T5542	17366-17374	NN	denotes	response
T5543	17375-17377	IN	denotes	to
T5544	17378-17381	DT	denotes	the
T5545	17382-17386	NN	denotes	loss
T5546	17387-17389	IN	denotes	of
T5547	17390-17393	DT	denotes	the
T5549	17394-17406	JJ	denotes	endocochlear
T5548	17407-17416	NN	denotes	potential
T5550	17416-17417	.	denotes	.
T5551	17417-17418	sentence	denotes
T8159	17428-17437	JJ	denotes	Potential
T8160	17437-17439	,	denotes	,
T8161	17439-17440	NN	denotes	K
T8163	17440-17441	SYM	denotes	+
T8162	17442-17456	NNS	denotes	concentrations
T8164	17457-17460	CC	denotes	and
T8165	17461-17473	NN	denotes	pigmentation
T8166	17474-17476	IN	denotes	of
T8167	17477-17482	NN	denotes	stria
T8168	17483-17493	NN	denotes	vascularis
T8169	17494-17496	IN	denotes	in
T8170	17497-17504	NN	denotes	Slc26a4
T8172	17504-17505	SYM	denotes	+
T8173	17505-17506	HYPH	denotes	/
T8174	17506-17507	SYM	denotes	+
T8175	17508-17511	CC	denotes	and
T8176	17512-17519	NN	denotes	Slc26a4
T8177	17519-17520	SYM	denotes	-
T8178	17520-17521	HYPH	denotes	/
T8179	17521-17522	SYM	denotes	-
T8171	17523-17527	NNS	denotes	mice
T8180	17527-17528	.	denotes	.
T8181	17528-17648	sentence	denotes	a: Endocochlear potential and K+ concentrations in endolymph and perilymph at the apex (A) and base (B) of the cochlea.
T8182	17529-17530	LS	denotes	a
T8184	17530-17532	:	denotes	:
T8185	17532-17544	JJ	denotes	Endocochlear
T8183	17545-17554	NN	denotes	potential
T8186	17555-17558	CC	denotes	and
T8187	17559-17560	NN	denotes	K
T8189	17560-17561	SYM	denotes	+
T8188	17562-17576	NNS	denotes	concentrations
T8190	17577-17579	IN	denotes	in
T8191	17580-17589	NN	denotes	endolymph
T8192	17590-17593	CC	denotes	and
T8193	17594-17603	NN	denotes	perilymph
T8194	17604-17606	IN	denotes	at
T8195	17607-17610	DT	denotes	the
T8196	17611-17615	NN	denotes	apex
T8197	17616-17617	-LRB-	denotes	(
T8198	17617-17618	NN	denotes	A
T8199	17618-17619	-RRB-	denotes	)
T8200	17620-17623	CC	denotes	and
T8201	17624-17628	NN	denotes	base
T8202	17629-17630	-LRB-	denotes	(
T8203	17630-17631	NN	denotes	B
T8204	17631-17632	-RRB-	denotes	)
T8205	17633-17635	IN	denotes	of
T8206	17636-17639	DT	denotes	the
T8207	17640-17647	NN	denotes	cochlea
T8208	17647-17648	.	denotes	.
T8209	17648-17707	sentence	denotes	Numbers adjacent to symbols denote number of measurements.
T8210	17649-17656	NNS	denotes	Numbers
T8212	17657-17665	JJ	denotes	adjacent
T8213	17666-17668	IN	denotes	to
T8214	17669-17676	NNS	denotes	symbols
T8211	17677-17683	VBP	denotes	denote
T8215	17684-17690	NN	denotes	number
T8216	17691-17693	IN	denotes	of
T8217	17694-17706	NNS	denotes	measurements
T8218	17706-17707	.	denotes	.
T8219	17707-17780	sentence	denotes	b-d: Pigmentation of stria vascularis in Slc26a4+/+ and Slc26a4-/- mice.
T8220	17708-17709	LS	denotes	b
T8222	17709-17710	SYM	denotes	-
T8223	17710-17711	LS	denotes	d
T8224	17711-17713	:	denotes	:
T8221	17713-17725	NN	denotes	Pigmentation
T8225	17726-17728	IN	denotes	of
T8226	17729-17734	NN	denotes	stria
T8227	17735-17745	NN	denotes	vascularis
T8228	17746-17748	IN	denotes	in
T8229	17749-17756	NN	denotes	Slc26a4
T8231	17756-17757	SYM	denotes	+
T8232	17757-17758	HYPH	denotes	/
T8233	17758-17759	SYM	denotes	+
T8234	17760-17763	CC	denotes	and
T8235	17764-17771	NN	denotes	Slc26a4
T8236	17771-17772	SYM	denotes	-
T8237	17772-17773	HYPH	denotes	/
T8238	17773-17774	SYM	denotes	-
T8230	17775-17779	NNS	denotes	mice
T8239	17779-17780	.	denotes	.
T8240	17780-17849	sentence	denotes	b: View of stria vascularis through the bony capsule of the cochlea.
T8241	17781-17782	LS	denotes	b
T8243	17782-17784	:	denotes	:
T8242	17784-17788	NN	denotes	View
T8244	17789-17791	IN	denotes	of
T8245	17792-17797	NN	denotes	stria
T8246	17798-17808	NN	denotes	vascularis
T8247	17809-17816	IN	denotes	through
T8248	17817-17820	DT	denotes	the
T8250	17821-17825	JJ	denotes	bony
T8249	17826-17833	NN	denotes	capsule
T8251	17834-17836	IN	denotes	of
T8252	17837-17840	DT	denotes	the
T8253	17841-17848	NN	denotes	cochlea
T8254	17848-17849	.	denotes	.
T8255	17849-17910	sentence	denotes	OW, oval window, RW, round window; arrows, stria vascularis.
T8256	17850-17852	NN	denotes	OW
T8257	17852-17854	,	denotes	,
T8258	17854-17858	JJ	denotes	oval
T8259	17859-17865	NN	denotes	window
T8260	17865-17867	,	denotes	,
T8261	17867-17869	NN	denotes	RW
T8262	17869-17871	,	denotes	,
T8263	17871-17876	JJ	denotes	round
T8264	17877-17883	NN	denotes	window
T8265	17883-17884	:	denotes	;
T8266	17885-17891	NNS	denotes	arrows
T8267	17891-17893	,	denotes	,
T8268	17893-17898	NN	denotes	stria
T8269	17899-17909	NN	denotes	vascularis
T8270	17909-17910	.	denotes	.
T8271	17910-17980	sentence	denotes	c-d: Whole-mounts of stria vascularis isolated from age-matched mice.
T8272	17911-17912	LS	denotes	c
T8274	17912-17913	SYM	denotes	-
T8275	17913-17914	LS	denotes	d
T8276	17914-17916	:	denotes	:
T8277	17916-17921	JJ	denotes	Whole
T8278	17921-17922	HYPH	denotes	-
T8273	17922-17928	NNS	denotes	mounts
T8279	17929-17931	IN	denotes	of
T8280	17932-17937	NN	denotes	stria
T8281	17938-17948	NN	denotes	vascularis
T8282	17949-17957	VBN	denotes	isolated
T8283	17958-17962	IN	denotes	from
T8284	17963-17966	NN	denotes	age
T8286	17966-17967	HYPH	denotes	-
T8285	17967-17974	VBN	denotes	matched
T8287	17975-17979	NNS	denotes	mice
T8288	17979-17980	.	denotes	.
T8289	17980-18079	sentence	denotes	c: Laser-scanning images, bar = 10 μm, d: Quantification of pigmentation based on optical density.
T8290	17981-17982	LS	denotes	c
T8292	17982-17984	:	denotes	:
T8293	17984-17989	NN	denotes	Laser
T8295	17989-17990	HYPH	denotes	-
T8296	17990-17998	VBG	denotes	scanning
T8294	17999-18005	NNS	denotes	images
T8297	18005-18007	,	denotes	,
T8298	18007-18010	NN	denotes	bar
T8299	18011-18012	SYM	denotes	=
T8300	18013-18015	CD	denotes	10
T8291	18016-18018	NN	denotes	μm
T8302	18018-18020	,	denotes	,
T8303	18020-18021	LS	denotes	d
T8304	18021-18023	:	denotes	:
T8301	18023-18037	NN	denotes	Quantification
T8305	18038-18040	IN	denotes	of
T8306	18041-18053	NN	denotes	pigmentation
T8307	18054-18059	VBN	denotes	based
T8308	18060-18062	IN	denotes	on
T8309	18063-18070	JJ	denotes	optical
T8310	18071-18078	NN	denotes	density
T8311	18078-18079	.	denotes	.
T5553	18080-18083	DT	denotes	The
T5552	18080-18183	sentence	denotes	The endocochlear potential is generated by stria vascularis in the lateral wall of the cochlea [17,23].
T5555	18084-18096	JJ	denotes	endocochlear
T5554	18097-18106	NN	denotes	potential
T5557	18107-18109	VBZ	denotes	is
T5556	18110-18119	VBN	denotes	generated
T5558	18120-18122	IN	denotes	by
T5559	18123-18128	NN	denotes	stria
T5560	18129-18139	NN	denotes	vascularis
T5561	18140-18142	IN	denotes	in
T5562	18143-18146	DT	denotes	the
T5564	18147-18154	JJ	denotes	lateral
T5563	18155-18159	NN	denotes	wall
T5565	18160-18162	IN	denotes	of
T5566	18163-18166	DT	denotes	the
T5567	18167-18174	NN	denotes	cochlea
T5568	18175-18176	-LRB-	denotes	[
T5570	18176-18178	CD	denotes	17
T5571	18178-18179	,	denotes	,
T5569	18179-18181	CD	denotes	23
T5572	18181-18182	-RRB-	denotes	]
T5573	18182-18183	.	denotes	.
T5574	18183-18397	sentence	denotes	The potential is generated across the basal cell barrier of stria vascularis by the K+ channel KCNJ10 located in intermediate cells [24], which are connected to basal cells by a high density of gap junctions [25].
T5575	18184-18187	DT	denotes	The
T5576	18188-18197	NN	denotes	potential
T5578	18198-18200	VBZ	denotes	is
T5577	18201-18210	VBN	denotes	generated
T5579	18211-18217	IN	denotes	across
T5580	18218-18221	DT	denotes	the
T5582	18222-18227	JJ	denotes	basal
T5583	18228-18232	NN	denotes	cell
T5581	18233-18240	NN	denotes	barrier
T5584	18241-18243	IN	denotes	of
T5585	18244-18249	NN	denotes	stria
T5586	18250-18260	NN	denotes	vascularis
T5587	18261-18263	IN	denotes	by
T5588	18264-18267	DT	denotes	the
T5590	18268-18269	NN	denotes	K
T5591	18269-18270	SYM	denotes	+
T5592	18271-18278	NN	denotes	channel
T5589	18279-18285	NN	denotes	KCNJ10
T5593	18286-18293	VBN	denotes	located
T5594	18294-18296	IN	denotes	in
T5595	18297-18309	JJ	denotes	intermediate
T5596	18310-18315	NNS	denotes	cells
T5597	18316-18317	-LRB-	denotes	[
T5598	18317-18319	CD	denotes	24
T5599	18319-18320	-RRB-	denotes	]
T5600	18320-18322	,	denotes	,
T5601	18322-18327	WDT	denotes	which
T5603	18328-18331	VBP	denotes	are
T5602	18332-18341	VBN	denotes	connected
T5604	18342-18344	IN	denotes	to
T5605	18345-18350	JJ	denotes	basal
T5606	18351-18356	NNS	denotes	cells
T5607	18357-18359	IN	denotes	by
T5608	18360-18361	DT	denotes	a
T5610	18362-18366	JJ	denotes	high
T5609	18367-18374	NN	denotes	density
T5611	18375-18377	IN	denotes	of
T5612	18378-18381	NN	denotes	gap
T5613	18382-18391	NNS	denotes	junctions
T5614	18392-18393	-LRB-	denotes	[
T5615	18393-18395	CD	denotes	25
T5616	18395-18396	-RRB-	denotes	]
T5617	18396-18397	.	denotes	.
T5618	18397-18603	sentence	denotes	Marginal cells of stria vascularis, which form the barrier toward endolymph, transport K+ from the intrastrial space into endolymph and keep the K+ concentration low adjacent to the KCNJ10 K+ channel [26].
T5619	18398-18406	JJ	denotes	Marginal
T5620	18407-18412	NNS	denotes	cells
T5622	18413-18415	IN	denotes	of
T5623	18416-18421	NN	denotes	stria
T5624	18422-18432	NN	denotes	vascularis
T5625	18432-18434	,	denotes	,
T5626	18434-18439	WDT	denotes	which
T5627	18440-18444	VBP	denotes	form
T5628	18445-18448	DT	denotes	the
T5629	18449-18456	NN	denotes	barrier
T5630	18457-18463	IN	denotes	toward
T5631	18464-18473	NN	denotes	endolymph
T5632	18473-18475	,	denotes	,
T5621	18475-18484	VBP	denotes	transport
T5633	18485-18486	NN	denotes	K
T5634	18486-18487	SYM	denotes	+
T5635	18488-18492	IN	denotes	from
T5636	18493-18496	DT	denotes	the
T5638	18497-18508	JJ	denotes	intrastrial
T5637	18509-18514	NN	denotes	space
T5639	18515-18519	IN	denotes	into
T5640	18520-18529	NN	denotes	endolymph
T5641	18530-18533	CC	denotes	and
T5642	18534-18538	VB	denotes	keep
T5643	18539-18542	DT	denotes	the
T5645	18543-18544	NN	denotes	K
T5646	18544-18545	SYM	denotes	+
T5644	18546-18559	NN	denotes	concentration
T5647	18560-18563	JJ	denotes	low
T5648	18564-18572	JJ	denotes	adjacent
T5649	18573-18575	IN	denotes	to
T5650	18576-18579	DT	denotes	the
T5652	18580-18586	NN	denotes	KCNJ10
T5653	18587-18588	NN	denotes	K
T5654	18588-18589	SYM	denotes	+
T5651	18590-18597	NN	denotes	channel
T5655	18598-18599	-LRB-	denotes	[
T5656	18599-18601	CD	denotes	26
T5657	18601-18602	-RRB-	denotes	]
T5658	18602-18603	.	denotes	.
T5659	18603-18869	sentence	denotes	To determine the cause of the loss of the endocochlear potential in Slc26a4-/- mice, we first determined whether intermediate cells are present in stria vascularis, since a loss of intermediate cells is known to lead to a loss of the endocochlear potential [27,28].
T5660	18604-18606	TO	denotes	To
T5661	18607-18616	VB	denotes	determine
T5663	18617-18620	DT	denotes	the
T5664	18621-18626	NN	denotes	cause
T5665	18627-18629	IN	denotes	of
T5666	18630-18633	DT	denotes	the
T5667	18634-18638	NN	denotes	loss
T5668	18639-18641	IN	denotes	of
T5669	18642-18645	DT	denotes	the
T5671	18646-18658	JJ	denotes	endocochlear
T5670	18659-18668	NN	denotes	potential
T5672	18669-18671	IN	denotes	in
T5673	18672-18679	NN	denotes	Slc26a4
T5675	18679-18680	SYM	denotes	-
T5676	18680-18681	HYPH	denotes	/
T5677	18681-18682	SYM	denotes	-
T5674	18683-18687	NNS	denotes	mice
T5678	18687-18689	,	denotes	,
T5679	18689-18691	PRP	denotes	we
T5680	18692-18697	RB	denotes	first
T5662	18698-18708	VBD	denotes	determined
T5681	18709-18716	IN	denotes	whether
T5683	18717-18729	JJ	denotes	intermediate
T5684	18730-18735	NNS	denotes	cells
T5682	18736-18739	VBP	denotes	are
T5685	18740-18747	JJ	denotes	present
T5686	18748-18750	IN	denotes	in
T5687	18751-18756	NN	denotes	stria
T5688	18757-18767	NN	denotes	vascularis
T5689	18767-18769	,	denotes	,
T5690	18769-18774	IN	denotes	since
T5692	18775-18776	DT	denotes	a
T5693	18777-18781	NN	denotes	loss
T5694	18782-18784	IN	denotes	of
T5695	18785-18797	JJ	denotes	intermediate
T5696	18798-18803	NNS	denotes	cells
T5697	18804-18806	VBZ	denotes	is
T5691	18807-18812	VBN	denotes	known
T5698	18813-18815	TO	denotes	to
T5699	18816-18820	VB	denotes	lead
T5700	18821-18823	IN	denotes	to
T5701	18824-18825	DT	denotes	a
T5702	18826-18830	NN	denotes	loss
T5703	18831-18833	IN	denotes	of
T5704	18834-18837	DT	denotes	the
T5706	18838-18850	JJ	denotes	endocochlear
T5705	18851-18860	NN	denotes	potential
T5707	18861-18862	-LRB-	denotes	[
T5709	18862-18864	CD	denotes	27
T5710	18864-18865	,	denotes	,
T5708	18865-18867	CD	denotes	28
T5711	18867-18868	-RRB-	denotes	]
T5712	18868-18869	.	denotes	.
T5713	18869-18947	sentence	denotes	Intermediate cells of stria vascularis were visualized by their pigmentation.
T5714	18870-18882	JJ	denotes	Intermediate
T5715	18883-18888	NNS	denotes	cells
T5717	18889-18891	IN	denotes	of
T5718	18892-18897	NN	denotes	stria
T5719	18898-18908	NN	denotes	vascularis
T5720	18909-18913	VBD	denotes	were
T5716	18914-18924	VBN	denotes	visualized
T5721	18925-18927	IN	denotes	by
T5722	18928-18933	PRP$	denotes	their
T5723	18934-18946	NN	denotes	pigmentation
T5724	18946-18947	.	denotes	.
T5725	18947-19074	sentence	denotes	Pigmentation was present in stria vascularis of Slc26a4-/- mice (Fig. 2b), which suggests that intermediate cells are present.
T5726	18948-18960	NN	denotes	Pigmentation
T5727	18961-18964	VBD	denotes	was
T5728	18965-18972	JJ	denotes	present
T5729	18973-18975	IN	denotes	in
T5730	18976-18981	NN	denotes	stria
T5731	18982-18992	NN	denotes	vascularis
T5732	18993-18995	IN	denotes	of
T5733	18996-19003	NN	denotes	Slc26a4
T5735	19003-19004	SYM	denotes	-
T5736	19004-19005	HYPH	denotes	/
T5737	19005-19006	SYM	denotes	-
T5734	19007-19011	NNS	denotes	mice
T5738	19012-19013	-LRB-	denotes	(
T5739	19013-19017	NN	denotes	Fig.
T5740	19018-19020	CD	denotes	2b
T5741	19020-19021	-RRB-	denotes	)
T5742	19021-19023	,	denotes	,
T5743	19023-19028	WDT	denotes	which
T5744	19029-19037	VBZ	denotes	suggests
T5745	19038-19042	IN	denotes	that
T5747	19043-19055	JJ	denotes	intermediate
T5748	19056-19061	NNS	denotes	cells
T5746	19062-19065	VBP	denotes	are
T5749	19066-19073	JJ	denotes	present
T5750	19073-19074	.	denotes	.
T5751	19074-19179	sentence	denotes	Interestingly, pigmentation of stria vascularis was much stronger in Slc26a4-/- than in Slc26a4+/+ mice.
T5752	19075-19088	RB	denotes	Interestingly
T5754	19088-19090	,	denotes	,
T5755	19090-19102	NN	denotes	pigmentation
T5756	19103-19105	IN	denotes	of
T5757	19106-19111	NN	denotes	stria
T5758	19112-19122	NN	denotes	vascularis
T5753	19123-19126	VBD	denotes	was
T5759	19127-19131	RB	denotes	much
T5760	19132-19140	JJR	denotes	stronger
T5761	19141-19143	IN	denotes	in
T5762	19144-19151	NN	denotes	Slc26a4
T5763	19151-19152	SYM	denotes	-
T5764	19152-19153	HYPH	denotes	/
T5765	19153-19154	SYM	denotes	-
T5766	19155-19159	IN	denotes	than
T5767	19160-19162	IN	denotes	in
T5768	19163-19170	NN	denotes	Slc26a4
T5770	19170-19171	SYM	denotes	+
T5771	19171-19172	HYPH	denotes	/
T5772	19172-19173	SYM	denotes	+
T5769	19174-19178	NNS	denotes	mice
T5773	19178-19179	.	denotes	.
T5774	19179-19543	sentence	denotes	To determine in greater detail the cause of the loss of the endocochlear potential in Slc26a4-/- mice, we isolated total RNA from stria vascularis and spiral ganglia of young (1–4 month) Slc26a4+/+ and both young and old (~12 month) Slc26a4-/- mice, assessed amounts of isolated tissues by quantification of 18S rRNA, and quantified the expression of KCNJ10 mRNA.
T5775	19180-19182	TO	denotes	To
T5776	19183-19192	VB	denotes	determine
T5778	19193-19195	IN	denotes	in
T5779	19196-19203	JJR	denotes	greater
T5780	19204-19210	NN	denotes	detail
T5781	19211-19214	DT	denotes	the
T5782	19215-19220	NN	denotes	cause
T5783	19221-19223	IN	denotes	of
T5784	19224-19227	DT	denotes	the
T5785	19228-19232	NN	denotes	loss
T5786	19233-19235	IN	denotes	of
T5787	19236-19239	DT	denotes	the
T5789	19240-19252	JJ	denotes	endocochlear
T5788	19253-19262	NN	denotes	potential
T5790	19263-19265	IN	denotes	in
T5791	19266-19273	NN	denotes	Slc26a4
T5793	19273-19274	SYM	denotes	-
T5794	19274-19275	HYPH	denotes	/
T5795	19275-19276	SYM	denotes	-
T5792	19277-19281	NNS	denotes	mice
T5796	19281-19283	,	denotes	,
T5797	19283-19285	PRP	denotes	we
T5777	19286-19294	VBD	denotes	isolated
T5798	19295-19300	JJ	denotes	total
T5799	19301-19304	NN	denotes	RNA
T5800	19305-19309	IN	denotes	from
T5801	19310-19315	NN	denotes	stria
T5802	19316-19326	NN	denotes	vascularis
T5803	19327-19330	CC	denotes	and
T5804	19331-19337	JJ	denotes	spiral
T5805	19338-19345	NNS	denotes	ganglia
T5806	19346-19348	IN	denotes	of
T5807	19349-19354	JJ	denotes	young
T5809	19355-19356	-LRB-	denotes	(
T5811	19356-19357	CD	denotes	1
T5813	19357-19358	SYM	denotes	–
T5812	19358-19359	CD	denotes	4
T5810	19360-19365	NN	denotes	month
T5814	19365-19366	-RRB-	denotes	)
T5808	19367-19374	NN	denotes	Slc26a4
T5815	19374-19375	SYM	denotes	+
T5816	19375-19376	HYPH	denotes	/
T5817	19376-19377	SYM	denotes	+
T5818	19378-19381	CC	denotes	and
T5819	19382-19386	CC	denotes	both
T5821	19387-19392	JJ	denotes	young
T5822	19393-19396	CC	denotes	and
T5823	19397-19400	JJ	denotes	old
T5824	19401-19402	-LRB-	denotes	(
T5826	19402-19403	SYM	denotes	~
T5827	19403-19405	CD	denotes	12
T5825	19406-19411	NN	denotes	month
T5828	19411-19412	-RRB-	denotes	)
T5820	19413-19420	NN	denotes	Slc26a4
T5830	19420-19421	SYM	denotes	-
T5831	19421-19422	HYPH	denotes	/
T5832	19422-19423	SYM	denotes	-
T5829	19424-19428	NNS	denotes	mice
T5833	19428-19430	,	denotes	,
T5834	19430-19438	VBN	denotes	assessed
T5835	19439-19446	NNS	denotes	amounts
T5836	19447-19449	IN	denotes	of
T5837	19450-19458	VBN	denotes	isolated
T5838	19459-19466	NNS	denotes	tissues
T5839	19467-19469	IN	denotes	by
T5840	19470-19484	NN	denotes	quantification
T5841	19485-19487	IN	denotes	of
T5842	19488-19491	NN	denotes	18S
T5843	19492-19496	NN	denotes	rRNA
T5844	19496-19498	,	denotes	,
T5845	19498-19501	CC	denotes	and
T5846	19502-19512	VBD	denotes	quantified
T5847	19513-19516	DT	denotes	the
T5848	19517-19527	NN	denotes	expression
T5849	19528-19530	IN	denotes	of
T5850	19531-19537	NN	denotes	KCNJ10
T5851	19538-19542	NN	denotes	mRNA
T5852	19542-19543	.	denotes	.
T5853	19543-19752	sentence	denotes	Quantities of stria vascularis isolated from these different mice were similar, since no significant differences were found in the numbers of 18S rRNA molecules (log(rRNA) = 9.46 ± 0.08, n = 17, pooled data).
T5854	19544-19554	NNS	denotes	Quantities
T5856	19555-19557	IN	denotes	of
T5857	19558-19563	NN	denotes	stria
T5858	19564-19574	NN	denotes	vascularis
T5859	19575-19583	VBN	denotes	isolated
T5860	19584-19588	IN	denotes	from
T5861	19589-19594	DT	denotes	these
T5863	19595-19604	JJ	denotes	different
T5862	19605-19609	NNS	denotes	mice
T5855	19610-19614	VBD	denotes	were
T5864	19615-19622	JJ	denotes	similar
T5865	19622-19624	,	denotes	,
T5866	19624-19629	IN	denotes	since
T5868	19630-19632	DT	denotes	no
T5870	19633-19644	JJ	denotes	significant
T5869	19645-19656	NNS	denotes	differences
T5871	19657-19661	VBD	denotes	were
T5867	19662-19667	VBN	denotes	found
T5872	19668-19670	IN	denotes	in
T5873	19671-19674	DT	denotes	the
T5874	19675-19682	NNS	denotes	numbers
T5875	19683-19685	IN	denotes	of
T5876	19686-19689	NN	denotes	18S
T5878	19690-19694	NN	denotes	rRNA
T5877	19695-19704	NNS	denotes	molecules
T5879	19705-19706	-LRB-	denotes	(
T5881	19706-19709	NN	denotes	log
T5883	19709-19710	-LRB-	denotes	(
T5882	19710-19714	NN	denotes	rRNA
T5885	19714-19715	-RRB-	denotes	)
T5886	19716-19717	SYM	denotes	=
T5887	19718-19722	CD	denotes	9.46
T5888	19723-19724	SYM	denotes	±
T5884	19725-19729	CD	denotes	0.08
T5889	19729-19731	,	denotes	,
T5890	19731-19732	NN	denotes	n
T5892	19733-19734	SYM	denotes	=
T5891	19735-19737	CD	denotes	17
T5893	19737-19739	,	denotes	,
T5894	19739-19745	JJ	denotes	pooled
T5880	19746-19750	NNS	denotes	data
T5895	19750-19751	-RRB-	denotes	)
T5896	19751-19752	.	denotes	.
T5897	19752-20026	sentence	denotes	In contrast, quantities of spiral ganglia isolated from young and old Slc26a4-/- mice (log(rRNA) = 9.04 ± 0.18, n = 4 and 9.29 ± 0.15, n = 6) were significantly smaller than in Slc26a4+/+ mice (log(rRNA) = 9.48 ± 0.19, n = 7), consistent with morphometric data (see below).
T5898	19753-19755	IN	denotes	In
T5900	19756-19764	NN	denotes	contrast
T5901	19764-19766	,	denotes	,
T5902	19766-19776	NNS	denotes	quantities
T5903	19777-19779	IN	denotes	of
T5904	19780-19786	JJ	denotes	spiral
T5905	19787-19794	NNS	denotes	ganglia
T5906	19795-19803	VBN	denotes	isolated
T5907	19804-19808	IN	denotes	from
T5908	19809-19814	JJ	denotes	young
T5910	19815-19818	CC	denotes	and
T5911	19819-19822	JJ	denotes	old
T5912	19823-19830	NN	denotes	Slc26a4
T5913	19830-19831	SYM	denotes	-
T5914	19831-19832	HYPH	denotes	/
T5915	19832-19833	SYM	denotes	-
T5909	19834-19838	NNS	denotes	mice
T5916	19839-19840	-LRB-	denotes	(
T5918	19840-19843	NN	denotes	log
T5920	19843-19844	-LRB-	denotes	(
T5919	19844-19848	NN	denotes	rRNA
T5921	19848-19849	-RRB-	denotes	)
T5922	19850-19851	SYM	denotes	=
T5923	19852-19856	CD	denotes	9.04
T5924	19857-19858	SYM	denotes	±
T5917	19859-19863	CD	denotes	0.18
T5925	19863-19865	,	denotes	,
T5927	19865-19866	NN	denotes	n
T5928	19867-19868	SYM	denotes	=
T5926	19869-19870	CD	denotes	4
T5929	19871-19874	CC	denotes	and
T5930	19875-19879	CD	denotes	9.29
T5932	19880-19881	SYM	denotes	±
T5931	19882-19886	CD	denotes	0.15
T5933	19886-19888	,	denotes	,
T5935	19888-19889	NN	denotes	n
T5936	19890-19891	SYM	denotes	=
T5934	19892-19893	CD	denotes	6
T5937	19893-19894	-RRB-	denotes	)
T5899	19895-19899	VBD	denotes	were
T5938	19900-19913	RB	denotes	significantly
T5939	19914-19921	JJR	denotes	smaller
T5940	19922-19926	IN	denotes	than
T5941	19927-19929	IN	denotes	in
T5942	19930-19937	NN	denotes	Slc26a4
T5944	19937-19938	SYM	denotes	+
T5945	19938-19939	HYPH	denotes	/
T5946	19939-19940	SYM	denotes	+
T5943	19941-19945	NNS	denotes	mice
T5947	19946-19947	-LRB-	denotes	(
T5949	19947-19950	NN	denotes	log
T5951	19950-19951	-LRB-	denotes	(
T5950	19951-19955	NN	denotes	rRNA
T5953	19955-19956	-RRB-	denotes	)
T5954	19957-19958	SYM	denotes	=
T5955	19959-19963	CD	denotes	9.48
T5956	19964-19965	SYM	denotes	±
T5952	19966-19970	CD	denotes	0.19
T5957	19970-19972	,	denotes	,
T5958	19972-19973	NN	denotes	n
T5959	19974-19975	SYM	denotes	=
T5948	19976-19977	CD	denotes	7
T5960	19977-19978	-RRB-	denotes	)
T5961	19978-19980	,	denotes	,
T5962	19980-19990	JJ	denotes	consistent
T5963	19991-19995	IN	denotes	with
T5964	19996-20008	JJ	denotes	morphometric
T5965	20009-20013	NNS	denotes	data
T5966	20014-20015	-LRB-	denotes	(
T5967	20015-20018	VB	denotes	see
T5968	20019-20024	RB	denotes	below
T5969	20024-20025	-RRB-	denotes	)
T5970	20025-20026	.	denotes	.
T5971	20026-20186	sentence	denotes	Expression of KCNJ10 mRNA was normal in stria vascularis and spiral ganglia of young Slc26a4-/- mice but significantly reduced in old Slc26a4-/- mice (Fig. 3).
T5972	20027-20037	NN	denotes	Expression
T5974	20038-20040	IN	denotes	of
T5975	20041-20047	NN	denotes	KCNJ10
T5976	20048-20052	NN	denotes	mRNA
T5973	20053-20056	VBD	denotes	was
T5977	20057-20063	JJ	denotes	normal
T5978	20064-20066	IN	denotes	in
T5979	20067-20072	NN	denotes	stria
T5980	20073-20083	NN	denotes	vascularis
T5981	20084-20087	CC	denotes	and
T5982	20088-20094	JJ	denotes	spiral
T5983	20095-20102	NNS	denotes	ganglia
T5984	20103-20105	IN	denotes	of
T5985	20106-20111	JJ	denotes	young
T5987	20112-20119	NN	denotes	Slc26a4
T5988	20119-20120	SYM	denotes	-
T5989	20120-20121	HYPH	denotes	/
T5990	20121-20122	SYM	denotes	-
T5986	20123-20127	NNS	denotes	mice
T5991	20128-20131	CC	denotes	but
T5992	20132-20145	RB	denotes	significantly
T5993	20146-20153	VBN	denotes	reduced
T5994	20154-20156	IN	denotes	in
T5995	20157-20160	JJ	denotes	old
T5997	20161-20168	NN	denotes	Slc26a4
T5998	20168-20169	SYM	denotes	-
T5999	20169-20170	HYPH	denotes	/
T6000	20170-20171	SYM	denotes	-
T5996	20172-20176	NNS	denotes	mice
T6001	20177-20178	-LRB-	denotes	(
T6002	20178-20182	NN	denotes	Fig.
T6003	20183-20184	CD	denotes	3
T6004	20184-20185	-RRB-	denotes	)
T6005	20185-20186	.	denotes	.
T6006	20186-20561	sentence	denotes	Quantifications of KCNQ1 and KCNQ4 mRNA were used to assess possible cross-contamination between the stria vascularis and spiral ganglia preparations based on the assumptions that (1) KCNQ1 is expressed in cells of the stria vascularis but not the spiral ganglia preparation and (2) KCNQ4 is expressed in cells of the spiral ganglia but not the stria vascularis preparation.
T6007	20187-20202	NNS	denotes	Quantifications
T6009	20203-20205	IN	denotes	of
T6010	20206-20211	NN	denotes	KCNQ1
T6012	20212-20215	CC	denotes	and
T6013	20216-20221	NN	denotes	KCNQ4
T6011	20222-20226	NN	denotes	mRNA
T6014	20227-20231	VBD	denotes	were
T6008	20232-20236	VBN	denotes	used
T6015	20237-20239	TO	denotes	to
T6016	20240-20246	VB	denotes	assess
T6017	20247-20255	JJ	denotes	possible
T6018	20256-20275	NN	denotes	cross-contamination
T6019	20276-20283	IN	denotes	between
T6020	20284-20287	DT	denotes	the
T6022	20288-20293	NN	denotes	stria
T6023	20294-20304	NN	denotes	vascularis
T6024	20305-20308	CC	denotes	and
T6025	20309-20315	JJ	denotes	spiral
T6026	20316-20323	NNS	denotes	ganglia
T6021	20324-20336	NNS	denotes	preparations
T6027	20337-20342	VBN	denotes	based
T6028	20343-20345	IN	denotes	on
T6029	20346-20349	DT	denotes	the
T6030	20350-20361	NNS	denotes	assumptions
T6031	20362-20366	IN	denotes	that
T6033	20367-20368	-LRB-	denotes	(
T6034	20368-20369	LS	denotes	1
T6035	20369-20370	-RRB-	denotes	)
T6036	20371-20376	NN	denotes	KCNQ1
T6037	20377-20379	VBZ	denotes	is
T6032	20380-20389	VBN	denotes	expressed
T6038	20390-20392	IN	denotes	in
T6039	20393-20398	NNS	denotes	cells
T6040	20399-20401	IN	denotes	of
T6041	20402-20405	DT	denotes	the
T6043	20406-20411	NN	denotes	stria
T6042	20412-20422	NN	denotes	vascularis
T6044	20423-20426	CC	denotes	but
T6045	20427-20430	RB	denotes	not
T6046	20431-20434	DT	denotes	the
T6048	20435-20441	JJ	denotes	spiral
T6049	20442-20449	NNS	denotes	ganglia
T6047	20450-20461	NN	denotes	preparation
T6050	20462-20465	CC	denotes	and
T6051	20466-20467	-LRB-	denotes	(
T6052	20467-20468	LS	denotes	2
T6054	20468-20469	-RRB-	denotes	)
T6055	20470-20475	NN	denotes	KCNQ4
T6056	20476-20478	VBZ	denotes	is
T6053	20479-20488	VBN	denotes	expressed
T6057	20489-20491	IN	denotes	in
T6058	20492-20497	NNS	denotes	cells
T6059	20498-20500	IN	denotes	of
T6060	20501-20504	DT	denotes	the
T6062	20505-20511	JJ	denotes	spiral
T6061	20512-20519	NNS	denotes	ganglia
T6063	20520-20523	CC	denotes	but
T6064	20524-20527	RB	denotes	not
T6065	20528-20531	DT	denotes	the
T6067	20532-20537	NN	denotes	stria
T6068	20538-20548	NN	denotes	vascularis
T6066	20549-20560	NN	denotes	preparation
T6069	20560-20561	.	denotes	.
T6070	20561-20762	sentence	denotes	The number of KCNQ1 mRNA molecules in stria vascularis was 24-fold greater than in spiral ganglia, and the number of KCNQ4 mRNA molecules in spiral ganglia was 5-fold greater than in stria vascularis.
T6071	20562-20565	DT	denotes	The
T6072	20566-20572	NN	denotes	number
T6074	20573-20575	IN	denotes	of
T6075	20576-20581	NN	denotes	KCNQ1
T6077	20582-20586	NN	denotes	mRNA
T6076	20587-20596	NNS	denotes	molecules
T6078	20597-20599	IN	denotes	in
T6079	20600-20605	NN	denotes	stria
T6080	20606-20616	NN	denotes	vascularis
T6073	20617-20620	VBD	denotes	was
T6081	20621-20623	CD	denotes	24
T6083	20623-20624	HYPH	denotes	-
T6084	20624-20628	RB	denotes	fold
T6082	20629-20636	JJR	denotes	greater
T6085	20637-20641	IN	denotes	than
T6086	20642-20644	IN	denotes	in
T6087	20645-20651	JJ	denotes	spiral
T6088	20652-20659	NNS	denotes	ganglia
T6089	20659-20661	,	denotes	,
T6090	20661-20664	CC	denotes	and
T6091	20665-20668	DT	denotes	the
T6092	20669-20675	NN	denotes	number
T6094	20676-20678	IN	denotes	of
T6095	20679-20684	NN	denotes	KCNQ4
T6097	20685-20689	NN	denotes	mRNA
T6096	20690-20699	NNS	denotes	molecules
T6098	20700-20702	IN	denotes	in
T6099	20703-20709	JJ	denotes	spiral
T6100	20710-20717	NNS	denotes	ganglia
T6093	20718-20721	VBD	denotes	was
T6101	20722-20723	CD	denotes	5
T6103	20723-20724	HYPH	denotes	-
T6104	20724-20728	RB	denotes	fold
T6102	20729-20736	JJR	denotes	greater
T6105	20737-20741	IN	denotes	than
T6106	20742-20744	IN	denotes	in
T6107	20745-20750	NN	denotes	stria
T6108	20751-20761	NN	denotes	vascularis
T6109	20761-20762	.	denotes	.
T6110	20762-20957	sentence	denotes	These observations validate our measurements of KCNJ10 and KCNQ1 by demonstrating that the microdissected preparations of stria vascularis and spiral ganglia were 98% and 78% pure, respectively.
T6111	20763-20768	DT	denotes	These
T6112	20769-20781	NNS	denotes	observations
T6113	20782-20790	VBP	denotes	validate
T6114	20791-20794	PRP$	denotes	our
T6115	20795-20807	NNS	denotes	measurements
T6116	20808-20810	IN	denotes	of
T6117	20811-20817	NN	denotes	KCNJ10
T6118	20818-20821	CC	denotes	and
T6119	20822-20827	NN	denotes	KCNQ1
T6120	20828-20830	IN	denotes	by
T6121	20831-20844	VBG	denotes	demonstrating
T6122	20845-20849	IN	denotes	that
T6124	20850-20853	DT	denotes	the
T6126	20854-20868	JJ	denotes	microdissected
T6125	20869-20881	NNS	denotes	preparations
T6127	20882-20884	IN	denotes	of
T6128	20885-20890	NN	denotes	stria
T6129	20891-20901	NN	denotes	vascularis
T6130	20902-20905	CC	denotes	and
T6131	20906-20912	JJ	denotes	spiral
T6132	20913-20920	NNS	denotes	ganglia
T6123	20921-20925	VBD	denotes	were
T6133	20926-20928	CD	denotes	98
T6134	20928-20929	NN	denotes	%
T6136	20930-20933	CC	denotes	and
T6137	20934-20936	CD	denotes	78
T6138	20936-20937	NN	denotes	%
T6135	20938-20942	JJ	denotes	pure
T6139	20942-20944	,	denotes	,
T6140	20944-20956	RB	denotes	respectively
T6141	20956-20957	.	denotes	.
T6142	20957-20958	sentence	denotes
T8435	20968-20982	NN	denotes	Quantification
T8436	20983-20985	IN	denotes	of
T8437	20986-20992	NN	denotes	KCNJ10
T8439	20993-20996	CC	denotes	and
T8440	20997-21002	NN	denotes	KCNQ1
T8441	21003-21007	NN	denotes	mRNA
T8438	21008-21018	NN	denotes	expression
T8442	21019-21021	IN	denotes	in
T8443	21022-21027	NN	denotes	stria
T8444	21028-21038	NN	denotes	vascularis
T8445	21039-21042	CC	denotes	and
T8446	21043-21049	JJ	denotes	spiral
T8447	21050-21057	NNS	denotes	ganglia
T8448	21058-21060	IN	denotes	of
T8449	21061-21068	NN	denotes	Slc26a4
T8451	21068-21069	SYM	denotes	+
T8452	21069-21070	HYPH	denotes	/
T8453	21070-21071	SYM	denotes	+
T8454	21072-21075	CC	denotes	and
T8455	21076-21083	NN	denotes	Slc26a4
T8456	21083-21084	SYM	denotes	-
T8457	21084-21085	HYPH	denotes	/
T8458	21085-21086	SYM	denotes	-
T8450	21087-21091	NNS	denotes	mice
T8459	21091-21092	.	denotes	.
T8460	21092-21187	sentence	denotes	a: Electropherogram of total RNA isolated from stria vascularis microdissected from one mouse.
T8461	21093-21094	LS	denotes	a
T8463	21094-21096	:	denotes	:
T8462	21096-21112	NN	denotes	Electropherogram
T8464	21113-21115	IN	denotes	of
T8465	21116-21121	JJ	denotes	total
T8466	21122-21125	NN	denotes	RNA
T8467	21126-21134	VBN	denotes	isolated
T8468	21135-21139	IN	denotes	from
T8469	21140-21145	NN	denotes	stria
T8470	21146-21156	NN	denotes	vascularis
T8471	21157-21171	VBN	denotes	microdissected
T8472	21172-21176	IN	denotes	from
T8473	21177-21180	CD	denotes	one
T8474	21181-21186	NN	denotes	mouse
T8475	21186-21187	.	denotes	.
T8476	21187-21332	sentence	denotes	The amount of total RNA was obtained from the total integral (shaded) and the amount of 18S rRNA was obtained from the integral of the 18S peak.
T8477	21188-21191	DT	denotes	The
T8478	21192-21198	NN	denotes	amount
T8480	21199-21201	IN	denotes	of
T8481	21202-21207	JJ	denotes	total
T8482	21208-21211	NN	denotes	RNA
T8483	21212-21215	VBD	denotes	was
T8479	21216-21224	VBN	denotes	obtained
T8484	21225-21229	IN	denotes	from
T8485	21230-21233	DT	denotes	the
T8487	21234-21239	JJ	denotes	total
T8486	21240-21248	NN	denotes	integral
T8488	21249-21250	-LRB-	denotes	(
T8489	21250-21256	JJ	denotes	shaded
T8490	21256-21257	-RRB-	denotes	)
T8491	21258-21261	CC	denotes	and
T8492	21262-21265	DT	denotes	the
T8493	21266-21272	NN	denotes	amount
T8495	21273-21275	IN	denotes	of
T8496	21276-21279	NN	denotes	18S
T8497	21280-21284	NN	denotes	rRNA
T8498	21285-21288	VBD	denotes	was
T8494	21289-21297	VBN	denotes	obtained
T8499	21298-21302	IN	denotes	from
T8500	21303-21306	DT	denotes	the
T8501	21307-21315	NN	denotes	integral
T8502	21316-21318	IN	denotes	of
T8503	21319-21322	DT	denotes	the
T8505	21323-21326	NN	denotes	18S
T8504	21327-21331	NN	denotes	peak
T8506	21331-21332	.	denotes	.
T8507	21332-21411	sentence	denotes	Sharp peaks representing 18S and 28S rRNA demonstrate the high quality of RNA.
T8508	21333-21338	JJ	denotes	Sharp
T8509	21339-21344	NNS	denotes	peaks
T8511	21345-21357	VBG	denotes	representing
T8512	21358-21361	NN	denotes	18S
T8514	21362-21365	CC	denotes	and
T8515	21366-21369	NN	denotes	28S
T8513	21370-21374	NN	denotes	rRNA
T8510	21375-21386	VBP	denotes	demonstrate
T8516	21387-21390	DT	denotes	the
T8518	21391-21395	JJ	denotes	high
T8517	21396-21403	NN	denotes	quality
T8519	21404-21406	IN	denotes	of
T8520	21407-21410	NN	denotes	RNA
T8521	21410-21411	.	denotes	.
T8522	21411-21558	sentence	denotes	Insert: Genotype of Slc26a4-/- mice was verified by the observation of one or few very large rhomboedric otoconia in the utricular macula (arrow).
T8523	21412-21418	NN	denotes	Insert
T8525	21418-21420	:	denotes	:
T8526	21420-21428	NN	denotes	Genotype
T8527	21429-21431	IN	denotes	of
T8528	21432-21439	NN	denotes	Slc26a4
T8530	21439-21440	SYM	denotes	-
T8531	21440-21441	HYPH	denotes	/
T8532	21441-21442	SYM	denotes	-
T8529	21443-21447	NNS	denotes	mice
T8533	21448-21451	VBD	denotes	was
T8524	21452-21460	VBN	denotes	verified
T8534	21461-21463	IN	denotes	by
T8535	21464-21467	DT	denotes	the
T8536	21468-21479	NN	denotes	observation
T8537	21480-21482	IN	denotes	of
T8538	21483-21486	CD	denotes	one
T8540	21487-21489	CC	denotes	or
T8541	21490-21493	JJ	denotes	few
T8542	21494-21498	RB	denotes	very
T8543	21499-21504	JJ	denotes	large
T8544	21505-21516	JJ	denotes	rhomboedric
T8539	21517-21525	NNS	denotes	otoconia
T8545	21526-21528	IN	denotes	in
T8546	21529-21532	DT	denotes	the
T8548	21533-21542	JJ	denotes	utricular
T8547	21543-21549	NN	denotes	macula
T8549	21550-21551	-LRB-	denotes	(
T8550	21551-21556	NN	denotes	arrow
T8551	21556-21557	-RRB-	denotes	)
T8552	21557-21558	.	denotes	.
T8553	21558-21601	sentence	denotes	A, crista ampullaris; U, utricular macula.
T8554	21559-21560	NN	denotes	A
T8555	21560-21562	,	denotes	,
T8556	21562-21568	NN	denotes	crista
T8557	21569-21579	NN	denotes	ampullaris
T8558	21579-21580	:	denotes	;
T8559	21581-21582	NN	denotes	U
T8560	21582-21584	,	denotes	,
T8561	21584-21593	JJ	denotes	utricular
T8562	21594-21600	NN	denotes	macula
T8563	21600-21601	.	denotes	.
T8564	21601-21620	sentence	denotes	Scale bar: 100 μm.
T8565	21602-21607	NN	denotes	Scale
T8566	21608-21611	NN	denotes	bar
T8568	21611-21613	:	denotes	:
T8569	21613-21616	CD	denotes	100
T8567	21617-21619	NNS	denotes	μm
T8570	21619-21620	.	denotes	.
T8571	21620-21713	sentence	denotes	b: Example of real-time RT-PCR data used for quantification of 18S, KCNJ10, KCNQ1 and KCNQ4.
T8572	21621-21622	LS	denotes	b
T8574	21622-21624	:	denotes	:
T8573	21624-21631	NN	denotes	Example
T8575	21632-21634	IN	denotes	of
T8576	21635-21639	JJ	denotes	real
T8578	21639-21640	HYPH	denotes	-
T8577	21640-21644	NN	denotes	time
T8580	21645-21647	NN	denotes	RT
T8582	21647-21648	HYPH	denotes	-
T8581	21648-21651	NN	denotes	PCR
T8579	21652-21656	NNS	denotes	data
T8583	21657-21661	VBN	denotes	used
T8584	21662-21665	IN	denotes	for
T8585	21666-21680	NN	denotes	quantification
T8586	21681-21683	IN	denotes	of
T8587	21684-21687	NN	denotes	18S
T8588	21687-21689	,	denotes	,
T8589	21689-21695	NN	denotes	KCNJ10
T8590	21695-21697	,	denotes	,
T8591	21697-21702	NN	denotes	KCNQ1
T8592	21703-21706	CC	denotes	and
T8593	21707-21712	NN	denotes	KCNQ4
T8594	21712-21713	.	denotes	.
T8595	21713-21780	sentence	denotes	Known quantities of 18S rRNA were used to calibrate the threshold.
T8596	21714-21719	JJ	denotes	Known
T8597	21720-21730	NNS	denotes	quantities
T8599	21731-21733	IN	denotes	of
T8600	21734-21737	NN	denotes	18S
T8601	21738-21742	NN	denotes	rRNA
T8602	21743-21747	VBD	denotes	were
T8598	21748-21752	VBN	denotes	used
T8603	21753-21755	TO	denotes	to
T8604	21756-21765	VB	denotes	calibrate
T8605	21766-21769	DT	denotes	the
T8606	21770-21779	NN	denotes	threshold
T8607	21779-21780	.	denotes	.
T8608	21780-21822	sentence	denotes	SV, stria vascularis; SG, spiral ganglia.
T8609	21781-21783	NN	denotes	SV
T8610	21783-21785	,	denotes	,
T8611	21785-21790	NN	denotes	stria
T8612	21791-21801	NN	denotes	vascularis
T8613	21801-21802	:	denotes	;
T8614	21803-21805	NN	denotes	SG
T8615	21805-21807	,	denotes	,
T8616	21807-21813	JJ	denotes	spiral
T8617	21814-21821	NNS	denotes	ganglia
T8618	21821-21822	.	denotes	.
T8619	21822-21920	sentence	denotes	c: Quantification of KCNJ10 and KCNQ1 mRNA in young Slc26a4+/+ and young and old Slc26a4-/- mice.
T8620	21823-21824	LS	denotes	c
T8622	21824-21826	:	denotes	:
T8621	21826-21840	NN	denotes	Quantification
T8623	21841-21843	IN	denotes	of
T8624	21844-21850	NN	denotes	KCNJ10
T8626	21851-21854	CC	denotes	and
T8627	21855-21860	NN	denotes	KCNQ1
T8625	21861-21865	NN	denotes	mRNA
T8628	21866-21868	IN	denotes	in
T8629	21869-21874	JJ	denotes	young
T8630	21875-21882	NN	denotes	Slc26a4
T8631	21882-21883	SYM	denotes	+
T8632	21883-21884	HYPH	denotes	/
T8633	21884-21885	SYM	denotes	+
T8634	21886-21889	CC	denotes	and
T8635	21890-21895	JJ	denotes	young
T8637	21896-21899	CC	denotes	and
T8638	21900-21903	JJ	denotes	old
T8639	21904-21911	NN	denotes	Slc26a4
T8640	21911-21912	SYM	denotes	-
T8641	21912-21913	HYPH	denotes	/
T8636	21913-21914	SYM	denotes	-
T8642	21915-21919	NNS	denotes	mice
T8643	21919-21920	.	denotes	.
T6144	21921-21924	DT	denotes	The
T6143	21921-21926	sentence	denotes	The p
T6145	21925-21926	NN	denotes	p
T6147	21926-21927	JJ	denotes	r
T6146	21926-21931	sentence	denotes	resen
T6148	21927-21929	NNS	denotes	es
T6149	21929-21931	VBG	denotes	en
T6151	21931-21932	NN	denotes	c
T6150	21931-21937	sentence	denotes	ce of
T6152	21932-21933	NNS	denotes	e
T6153	21934-21935	NN	denotes	o
T6154	21935-21936	IN	denotes	f
T6156	21937-21943	NN	denotes	KCNJ10
T6155	21937-21944	sentence	denotes	KCNJ10
T6157	21943-21944	,	denotes
T6159	21944-21948	NN	denotes	mRNA
T6158	21944-21952	sentence	denotes	mRNA in
T6160	21949-21951	IN	denotes	in
T6162	21952-21953	NN	denotes	s
T6161	21952-22045	sentence	denotes	stria vascularis of Slc26a4-/- mice supports the finding that intermediate cells are present.
T6164	21953-21957	NN	denotes	tria
T6165	21958-21968	NN	denotes	vascularis
T6166	21969-21971	IN	denotes	of
T6167	21972-21979	NN	denotes	Slc26a4
T6169	21979-21980	SYM	denotes	-
T6170	21980-21981	HYPH	denotes	/
T6171	21981-21982	SYM	denotes	-
T6168	21983-21987	NNS	denotes	mice
T6163	21988-21996	VBZ	denotes	supports
T6172	21997-22000	DT	denotes	the
T6173	22001-22008	NN	denotes	finding
T6174	22009-22013	IN	denotes	that
T6176	22014-22026	JJ	denotes	intermediate
T6177	22027-22032	NNS	denotes	cells
T6175	22033-22036	VBP	denotes	are
T6178	22037-22044	JJ	denotes	present
T6179	22044-22045	.	denotes	.
T6180	22045-22149	sentence	denotes	Expression of the KCNJ10 protein was assessed in young Slc26a4-/- mice by confocal immunocytochemistry.
T6181	22046-22056	NN	denotes	Expression
T6183	22057-22059	IN	denotes	of
T6184	22060-22063	DT	denotes	the
T6186	22064-22070	NN	denotes	KCNJ10
T6185	22071-22078	NN	denotes	protein
T6187	22079-22082	VBD	denotes	was
T6182	22083-22091	VBN	denotes	assessed
T6188	22092-22094	IN	denotes	in
T6189	22095-22100	JJ	denotes	young
T6191	22101-22108	NN	denotes	Slc26a4
T6192	22108-22109	SYM	denotes	-
T6193	22109-22110	HYPH	denotes	/
T6194	22110-22111	SYM	denotes	-
T6190	22112-22116	NNS	denotes	mice
T6195	22117-22119	IN	denotes	by
T6196	22120-22128	JJ	denotes	confocal
T6197	22129-22148	NN	denotes	immunocytochemistry
T6198	22148-22149	.	denotes	.
T6199	22149-22267	sentence	denotes	Interestingly, expression of the KCNJ10 protein was absent in stria vascularis but normal in spiral ganglia (Fig. 4).
T6200	22150-22163	RB	denotes	Interestingly
T6202	22163-22165	,	denotes	,
T6203	22165-22175	NN	denotes	expression
T6204	22176-22178	IN	denotes	of
T6205	22179-22182	DT	denotes	the
T6207	22183-22189	NN	denotes	KCNJ10
T6206	22190-22197	NN	denotes	protein
T6201	22198-22201	VBD	denotes	was
T6208	22202-22208	JJ	denotes	absent
T6209	22209-22211	IN	denotes	in
T6210	22212-22217	NN	denotes	stria
T6211	22218-22228	NN	denotes	vascularis
T6212	22229-22232	CC	denotes	but
T6213	22233-22239	JJ	denotes	normal
T6214	22240-22242	IN	denotes	in
T6215	22243-22249	JJ	denotes	spiral
T6216	22250-22257	NNS	denotes	ganglia
T6217	22258-22259	-LRB-	denotes	(
T6218	22259-22263	NN	denotes	Fig.
T6219	22264-22265	CD	denotes	4
T6220	22265-22266	-RRB-	denotes	)
T6221	22266-22267	.	denotes	.
T6222	22267-22394	sentence	denotes	The absence of the KCNJ10 K+ channel in stria vascularis is sufficient to explain the loss of the endocochlear potential [17].
T6223	22268-22271	DT	denotes	The
T6224	22272-22279	NN	denotes	absence
T6226	22280-22282	IN	denotes	of
T6227	22283-22286	DT	denotes	the
T6229	22287-22293	NN	denotes	KCNJ10
T6230	22294-22295	NN	denotes	K
T6231	22295-22296	SYM	denotes	+
T6228	22297-22304	NN	denotes	channel
T6232	22305-22307	IN	denotes	in
T6233	22308-22313	NN	denotes	stria
T6234	22314-22324	NN	denotes	vascularis
T6225	22325-22327	VBZ	denotes	is
T6235	22328-22338	JJ	denotes	sufficient
T6236	22339-22341	TO	denotes	to
T6237	22342-22349	VB	denotes	explain
T6238	22350-22353	DT	denotes	the
T6239	22354-22358	NN	denotes	loss
T6240	22359-22361	IN	denotes	of
T6241	22362-22365	DT	denotes	the
T6243	22366-22378	JJ	denotes	endocochlear
T6242	22379-22388	NN	denotes	potential
T6244	22389-22390	-LRB-	denotes	[
T6245	22390-22392	CD	denotes	17
T6246	22392-22393	-RRB-	denotes	]
T6247	22393-22394	.	denotes	.
T6248	22394-22395	sentence	denotes
T8717	22405-22412	NN	denotes	Protein
T8718	22413-22425	NN	denotes	localization
T8719	22426-22428	IN	denotes	of
T8720	22429-22435	NN	denotes	KCNJ10
T8721	22436-22438	IN	denotes	in
T8722	22439-22442	DT	denotes	the
T8723	22443-22450	NN	denotes	cochlea
T8724	22451-22453	IN	denotes	of
T8725	22454-22461	NN	denotes	Slc26a4
T8727	22461-22462	SYM	denotes	+
T8728	22462-22463	HYPH	denotes	/
T8729	22463-22464	SYM	denotes	+
T8730	22465-22468	CC	denotes	and
T8731	22469-22476	NN	denotes	Slc26a4
T8732	22476-22477	SYM	denotes	-
T8733	22477-22478	HYPH	denotes	/
T8734	22478-22479	SYM	denotes	-
T8726	22480-22484	NNS	denotes	mice
T8735	22484-22485	.	denotes	.
T8736	22485-22523	sentence	denotes	a: Overview of cochlea; bar = 100 μm.
T8737	22486-22487	LS	denotes	a
T8739	22487-22489	:	denotes	:
T8740	22489-22497	NN	denotes	Overview
T8741	22498-22500	IN	denotes	of
T8742	22501-22508	NN	denotes	cochlea
T8743	22508-22509	:	denotes	;
T8744	22510-22513	NN	denotes	bar
T8745	22514-22515	SYM	denotes	=
T8746	22516-22519	CD	denotes	100
T8738	22520-22522	NNS	denotes	μm
T8747	22522-22523	.	denotes	.
T8748	22523-22614	sentence	denotes	Compare to Fig. 1a to note the enlarged scala media and the distended Reissner's membrane.
T8749	22524-22531	VB	denotes	Compare
T8750	22532-22534	IN	denotes	to
T8751	22535-22539	NN	denotes	Fig.
T8752	22540-22542	CD	denotes	1a
T8753	22543-22545	TO	denotes	to
T8754	22546-22550	VB	denotes	note
T8755	22551-22554	DT	denotes	the
T8757	22555-22563	JJ	denotes	enlarged
T8758	22564-22569	NN	denotes	scala
T8756	22570-22575	NNS	denotes	media
T8759	22576-22579	CC	denotes	and
T8760	22580-22583	DT	denotes	the
T8762	22584-22593	JJ	denotes	distended
T8763	22594-22602	NNP	denotes	Reissner
T8764	22602-22604	POS	denotes	's
T8761	22605-22613	NN	denotes	membrane
T8765	22613-22614	.	denotes	.
T8766	22614-22702	sentence	denotes	b-c: Detail of lateral wall and spiral ganglia (insert); main bar: 10 μm, insert: 5 μm.
T8767	22615-22616	LS	denotes	b
T8769	22616-22617	HYPH	denotes	-
T8770	22617-22618	LS	denotes	c
T8771	22618-22620	:	denotes	:
T8768	22620-22626	NN	denotes	Detail
T8772	22627-22629	IN	denotes	of
T8773	22630-22637	JJ	denotes	lateral
T8774	22638-22642	NN	denotes	wall
T8775	22643-22646	CC	denotes	and
T8776	22647-22653	JJ	denotes	spiral
T8777	22654-22661	NNS	denotes	ganglia
T8778	22662-22663	-LRB-	denotes	(
T8779	22663-22669	NN	denotes	insert
T8780	22669-22670	-RRB-	denotes	)
T8781	22670-22671	:	denotes	;
T8782	22672-22676	JJ	denotes	main
T8783	22677-22680	NN	denotes	bar
T8785	22680-22682	:	denotes	:
T8786	22682-22684	CD	denotes	10
T8784	22685-22687	NNS	denotes	μm
T8787	22687-22689	,	denotes	,
T8788	22689-22695	NN	denotes	insert
T8790	22695-22697	:	denotes	:
T8791	22697-22698	CD	denotes	5
T8789	22699-22701	NNS	denotes	μm
T8792	22701-22702	.	denotes	.
T8793	22702-22813	sentence	denotes	Expression of KCNJ10 in Slc26a4-/- mice was absent in stria vascularis but unchanged in spiral ganglion cells.
T8794	22703-22713	NN	denotes	Expression
T8796	22714-22716	IN	denotes	of
T8797	22717-22723	NN	denotes	KCNJ10
T8798	22724-22726	IN	denotes	in
T8799	22727-22734	NN	denotes	Slc26a4
T8801	22734-22735	SYM	denotes	-
T8802	22735-22736	HYPH	denotes	/
T8803	22736-22737	SYM	denotes	-
T8800	22738-22742	NNS	denotes	mice
T8795	22743-22746	VBD	denotes	was
T8804	22747-22753	JJ	denotes	absent
T8805	22754-22756	IN	denotes	in
T8806	22757-22762	NN	denotes	stria
T8807	22763-22773	NN	denotes	vascularis
T8808	22774-22777	CC	denotes	but
T8809	22778-22787	JJ	denotes	unchanged
T8810	22788-22790	IN	denotes	in
T8811	22791-22797	JJ	denotes	spiral
T8812	22798-22806	NN	denotes	ganglion
T8813	22807-22812	NNS	denotes	cells
T8814	22812-22813	.	denotes	.
T8815	22813-22945	sentence	denotes	RM, Reissner's membrane, SV, stria vascularis; SP, spiral prominence; SL, spiral ligament; LIM, spiral limbus; SG, spiral ganglion.
T8816	22814-22816	NNP	denotes	RM
T8817	22816-22818	,	denotes	,
T8818	22818-22826	NNP	denotes	Reissner
T8820	22826-22828	POS	denotes	's
T8819	22829-22837	NN	denotes	membrane
T8821	22837-22839	,	denotes	,
T8822	22839-22841	NN	denotes	SV
T8823	22841-22843	,	denotes	,
T8824	22843-22848	NN	denotes	stria
T8825	22849-22859	NN	denotes	vascularis
T8826	22859-22860	:	denotes	;
T8827	22861-22863	NN	denotes	SP
T8828	22863-22865	,	denotes	,
T8829	22865-22871	JJ	denotes	spiral
T8830	22872-22882	NN	denotes	prominence
T8831	22882-22883	:	denotes	;
T8832	22884-22886	NN	denotes	SL
T8833	22886-22888	,	denotes	,
T8834	22888-22894	JJ	denotes	spiral
T8835	22895-22903	NN	denotes	ligament
T8836	22903-22904	:	denotes	;
T8837	22905-22908	NN	denotes	LIM
T8838	22908-22910	,	denotes	,
T8839	22910-22916	JJ	denotes	spiral
T8840	22917-22923	NN	denotes	limbus
T8841	22923-22924	:	denotes	;
T8842	22925-22927	NN	denotes	SG
T8843	22927-22929	,	denotes	,
T8844	22929-22935	JJ	denotes	spiral
T8845	22936-22944	NN	denotes	ganglion
T8846	22944-22945	.	denotes	.
T6250	22946-22958	JJ	denotes	Histological
T6249	22946-23147	sentence	denotes	Histological evaluation of cryosections revealed an enlargement of scala media with a large bulging of Reissner's membrane and an apparent degeneration of the organ of Corti, as described earlier [14].
T6251	22959-22969	NN	denotes	evaluation
T6253	22970-22972	IN	denotes	of
T6254	22973-22985	NNS	denotes	cryosections
T6252	22986-22994	VBD	denotes	revealed
T6255	22995-22997	DT	denotes	an
T6256	22998-23009	NN	denotes	enlargement
T6257	23010-23012	IN	denotes	of
T6258	23013-23018	NN	denotes	scala
T6259	23019-23024	NNS	denotes	media
T6260	23025-23029	IN	denotes	with
T6261	23030-23031	DT	denotes	a
T6263	23032-23037	JJ	denotes	large
T6262	23038-23045	NN	denotes	bulging
T6264	23046-23048	IN	denotes	of
T6265	23049-23057	NNP	denotes	Reissner
T6267	23057-23059	POS	denotes	's
T6266	23060-23068	NN	denotes	membrane
T6268	23069-23072	CC	denotes	and
T6269	23073-23075	DT	denotes	an
T6271	23076-23084	JJ	denotes	apparent
T6270	23085-23097	NN	denotes	degeneration
T6272	23098-23100	IN	denotes	of
T6273	23101-23104	DT	denotes	the
T6274	23105-23110	NN	denotes	organ
T6275	23111-23113	IN	denotes	of
T6276	23114-23119	NNP	denotes	Corti
T6277	23119-23121	,	denotes	,
T6278	23121-23123	IN	denotes	as
T6279	23124-23133	VBN	denotes	described
T6280	23134-23141	RBR	denotes	earlier
T6281	23142-23143	-LRB-	denotes	[
T6282	23143-23145	CD	denotes	14
T6283	23145-23146	-RRB-	denotes	]
T6284	23146-23147	.	denotes	.
T6285	23147-23298	sentence	denotes	Tissue height of stria vascularis was normal (Fig. 5), consistent with the similar numbers of 18S rRNA molecules in isolated preparations (see above).
T6286	23148-23154	NN	denotes	Tissue
T6287	23155-23161	NN	denotes	height
T6289	23162-23164	IN	denotes	of
T6290	23165-23170	NN	denotes	stria
T6291	23171-23181	NN	denotes	vascularis
T6288	23182-23185	VBD	denotes	was
T6292	23186-23192	JJ	denotes	normal
T6293	23193-23194	-LRB-	denotes	(
T6294	23194-23198	NN	denotes	Fig.
T6295	23199-23200	CD	denotes	5
T6296	23200-23201	-RRB-	denotes	)
T6297	23201-23203	,	denotes	,
T6298	23203-23213	JJ	denotes	consistent
T6299	23214-23218	IN	denotes	with
T6300	23219-23222	DT	denotes	the
T6302	23223-23230	JJ	denotes	similar
T6301	23231-23238	NNS	denotes	numbers
T6303	23239-23241	IN	denotes	of
T6304	23242-23245	NN	denotes	18S
T6306	23246-23250	NN	denotes	rRNA
T6305	23251-23260	NNS	denotes	molecules
T6307	23261-23263	IN	denotes	in
T6308	23264-23272	VBN	denotes	isolated
T6309	23273-23285	NNS	denotes	preparations
T6310	23286-23287	-LRB-	denotes	(
T6311	23287-23290	VB	denotes	see
T6312	23291-23296	RB	denotes	above
T6313	23296-23297	-RRB-	denotes	)
T6314	23297-23298	.	denotes	.
T6315	23298-23399	sentence	denotes	The absence of a change in tissue height is consistent with the presence of intermediate cells [28].
T6316	23299-23302	DT	denotes	The
T6317	23303-23310	NN	denotes	absence
T6319	23311-23313	IN	denotes	of
T6320	23314-23315	DT	denotes	a
T6321	23316-23322	NN	denotes	change
T6322	23323-23325	IN	denotes	in
T6323	23326-23332	NN	denotes	tissue
T6324	23333-23339	NN	denotes	height
T6318	23340-23342	VBZ	denotes	is
T6325	23343-23353	JJ	denotes	consistent
T6326	23354-23358	IN	denotes	with
T6327	23359-23362	DT	denotes	the
T6328	23363-23371	NN	denotes	presence
T6329	23372-23374	IN	denotes	of
T6330	23375-23387	JJ	denotes	intermediate
T6331	23388-23393	NNS	denotes	cells
T6332	23394-23395	-LRB-	denotes	[
T6333	23395-23397	CD	denotes	28
T6334	23397-23398	-RRB-	denotes	]
T6335	23398-23399	.	denotes	.
T6336	23399-23529	sentence	denotes	Further, we observed an apparent loss of tissue masses in areas that are normally occupied primarily by type I and II fibrocytes.
T6337	23400-23407	RB	denotes	Further
T6339	23407-23409	,	denotes	,
T6340	23409-23411	PRP	denotes	we
T6338	23412-23420	VBD	denotes	observed
T6341	23421-23423	DT	denotes	an
T6343	23424-23432	JJ	denotes	apparent
T6342	23433-23437	NN	denotes	loss
T6344	23438-23440	IN	denotes	of
T6345	23441-23447	NN	denotes	tissue
T6346	23448-23454	NNS	denotes	masses
T6347	23455-23457	IN	denotes	in
T6348	23458-23463	NNS	denotes	areas
T6349	23464-23468	WDT	denotes	that
T6351	23469-23472	VBP	denotes	are
T6352	23473-23481	RB	denotes	normally
T6350	23482-23490	VBN	denotes	occupied
T6353	23491-23500	RB	denotes	primarily
T6354	23501-23503	IN	denotes	by
T6355	23504-23508	NN	denotes	type
T6356	23509-23510	CD	denotes	I
T6358	23511-23514	CC	denotes	and
T6359	23515-23517	CD	denotes	II
T6357	23518-23528	NNS	denotes	fibrocytes
T6360	23528-23529	.	denotes	.
T6361	23529-23646	sentence	denotes	Spiral prominence in Slc26a4-/- mice was less prominent, spiral ligament thinner and spiral limbus flatter (Fig. 5).
T6362	23530-23536	JJ	denotes	Spiral
T6363	23537-23547	NN	denotes	prominence
T6365	23548-23550	IN	denotes	in
T6366	23551-23558	NN	denotes	Slc26a4
T6368	23558-23559	SYM	denotes	-
T6369	23559-23560	HYPH	denotes	/
T6370	23560-23561	SYM	denotes	-
T6367	23562-23566	NNS	denotes	mice
T6364	23567-23570	VBD	denotes	was
T6371	23571-23575	RBR	denotes	less
T6372	23576-23585	JJ	denotes	prominent
T6373	23585-23587	,	denotes	,
T6374	23587-23593	JJ	denotes	spiral
T6375	23594-23602	NN	denotes	ligament
T6376	23603-23610	JJR	denotes	thinner
T6377	23611-23614	CC	denotes	and
T6378	23615-23621	JJ	denotes	spiral
T6379	23622-23628	NN	denotes	limbus
T6380	23629-23636	JJR	denotes	flatter
T6381	23637-23638	-LRB-	denotes	(
T6382	23638-23642	NN	denotes	Fig.
T6383	23643-23644	CD	denotes	5
T6384	23644-23645	-RRB-	denotes	)
T6385	23645-23646	.	denotes	.
T6386	23646-23839	sentence	denotes	The observation that tissue masses were lost in the spiral limbus region is consistent with the finding of a reduced number of 18S rRNA molecules in the spiral ganglia preparation (see above).
T6387	23647-23650	DT	denotes	The
T6388	23651-23662	NN	denotes	observation
T6390	23663-23667	IN	denotes	that
T6392	23668-23674	NN	denotes	tissue
T6393	23675-23681	NNS	denotes	masses
T6394	23682-23686	VBD	denotes	were
T6391	23687-23691	VBN	denotes	lost
T6395	23692-23694	IN	denotes	in
T6396	23695-23698	DT	denotes	the
T6398	23699-23705	JJ	denotes	spiral
T6399	23706-23712	NN	denotes	limbus
T6397	23713-23719	NN	denotes	region
T6389	23720-23722	VBZ	denotes	is
T6400	23723-23733	JJ	denotes	consistent
T6401	23734-23738	IN	denotes	with
T6402	23739-23742	DT	denotes	the
T6403	23743-23750	NN	denotes	finding
T6404	23751-23753	IN	denotes	of
T6405	23754-23755	DT	denotes	a
T6407	23756-23763	VBN	denotes	reduced
T6406	23764-23770	NN	denotes	number
T6408	23771-23773	IN	denotes	of
T6409	23774-23777	NN	denotes	18S
T6410	23778-23782	NN	denotes	rRNA
T6411	23783-23792	NNS	denotes	molecules
T6412	23793-23795	IN	denotes	in
T6413	23796-23799	DT	denotes	the
T6415	23800-23806	JJ	denotes	spiral
T6416	23807-23814	NNS	denotes	ganglia
T6414	23815-23826	NN	denotes	preparation
T6417	23827-23828	-LRB-	denotes	(
T6418	23828-23831	VB	denotes	see
T6419	23832-23837	RB	denotes	above
T6420	23837-23838	-RRB-	denotes	)
T6421	23838-23839	.	denotes	.
T6422	23839-24030	sentence	denotes	In addition, we observed an apparent degeneration of stria vascularis, including an increase in pigmentation and an irregular pattern of the tight junctions of marginal cells (Fig. 2c, 6,7).
T6423	23840-23842	IN	denotes	In
T6425	23843-23851	NN	denotes	addition
T6426	23851-23853	,	denotes	,
T6427	23853-23855	PRP	denotes	we
T6424	23856-23864	VBD	denotes	observed
T6428	23865-23867	DT	denotes	an
T6430	23868-23876	JJ	denotes	apparent
T6429	23877-23889	NN	denotes	degeneration
T6431	23890-23892	IN	denotes	of
T6432	23893-23898	NN	denotes	stria
T6433	23899-23909	NN	denotes	vascularis
T6434	23909-23911	,	denotes	,
T6435	23911-23920	VBG	denotes	including
T6436	23921-23923	DT	denotes	an
T6437	23924-23932	NN	denotes	increase
T6438	23933-23935	IN	denotes	in
T6439	23936-23948	NN	denotes	pigmentation
T6440	23949-23952	CC	denotes	and
T6441	23953-23955	DT	denotes	an
T6443	23956-23965	JJ	denotes	irregular
T6442	23966-23973	NN	denotes	pattern
T6444	23974-23976	IN	denotes	of
T6445	23977-23980	DT	denotes	the
T6447	23981-23986	JJ	denotes	tight
T6446	23987-23996	NNS	denotes	junctions
T6448	23997-23999	IN	denotes	of
T6449	24000-24008	JJ	denotes	marginal
T6450	24009-24014	NNS	denotes	cells
T6451	24015-24016	-LRB-	denotes	(
T6453	24016-24020	NN	denotes	Fig.
T6454	24021-24023	CD	denotes	2c
T6455	24023-24025	,	denotes	,
T6456	24025-24026	CD	denotes	6
T6457	24026-24027	,	denotes	,
T6452	24027-24028	CD	denotes	7
T6458	24028-24029	-RRB-	denotes	)
T6459	24029-24030	.	denotes	.
T6460	24030-24085	sentence	denotes	Tight junctions were visualized by F-actin expression.
T6461	24031-24036	JJ	denotes	Tight
T6462	24037-24046	NNS	denotes	junctions
T6464	24047-24051	VBD	denotes	were
T6463	24052-24062	VBN	denotes	visualized
T6465	24063-24065	IN	denotes	by
T6466	24066-24067	NN	denotes	F
T6468	24067-24068	HYPH	denotes	-
T6467	24068-24073	NN	denotes	actin
T6469	24074-24084	NN	denotes	expression
T6470	24084-24085	.	denotes	.
T6471	24085-24137	sentence	denotes	Marginal cells appeared to form a continuous layer.
T6472	24086-24094	JJ	denotes	Marginal
T6473	24095-24100	NNS	denotes	cells
T6474	24101-24109	VBD	denotes	appeared
T6475	24110-24112	TO	denotes	to
T6476	24113-24117	VB	denotes	form
T6477	24118-24119	DT	denotes	a
T6479	24120-24130	JJ	denotes	continuous
T6478	24131-24136	NN	denotes	layer
T6480	24136-24137	.	denotes	.
T6481	24137-24138	sentence	denotes
T8911	24148-24160	JJ	denotes	Morphometric
T8912	24161-24169	NN	denotes	analysis
T8913	24170-24172	IN	denotes	of
T8914	24173-24181	JJ	denotes	cochlear
T8916	24182-24188	NN	denotes	tissue
T8915	24189-24195	NNS	denotes	masses
T8917	24196-24198	IN	denotes	in
T8918	24199-24206	NN	denotes	Slc26a4
T8920	24206-24207	SYM	denotes	+
T8921	24207-24208	HYPH	denotes	/
T8922	24208-24209	SYM	denotes	+
T8923	24210-24213	CC	denotes	and
T8924	24214-24221	NN	denotes	Slc26a4
T8925	24221-24222	SYM	denotes	-
T8926	24222-24223	HYPH	denotes	/
T8927	24223-24224	SYM	denotes	-
T8919	24225-24229	NNS	denotes	mice
T8928	24229-24230	.	denotes	.
T8929	24230-24260	sentence	denotes	a: locations of measurements.
T8930	24231-24232	LS	denotes	a
T8932	24232-24234	:	denotes	:
T8931	24234-24243	NNS	denotes	locations
T8933	24244-24246	IN	denotes	of
T8934	24247-24259	NNS	denotes	measurements
T8935	24259-24260	.	denotes	.
T8936	24260-24398	sentence	denotes	Thickness of stria vascularis (SV) was obtained as average of three distance measurements perpendicular to the surface of marginal cells.
T8937	24261-24270	NN	denotes	Thickness
T8939	24271-24273	IN	denotes	of
T8940	24274-24279	NN	denotes	stria
T8941	24280-24290	NN	denotes	vascularis
T8942	24291-24292	-LRB-	denotes	(
T8943	24292-24294	NN	denotes	SV
T8944	24294-24295	-RRB-	denotes	)
T8945	24296-24299	VBD	denotes	was
T8938	24300-24308	VBN	denotes	obtained
T8946	24309-24311	IN	denotes	as
T8947	24312-24319	NN	denotes	average
T8948	24320-24322	IN	denotes	of
T8949	24323-24328	CD	denotes	three
T8951	24329-24337	NN	denotes	distance
T8950	24338-24350	NNS	denotes	measurements
T8952	24351-24364	JJ	denotes	perpendicular
T8953	24365-24367	IN	denotes	to
T8954	24368-24371	DT	denotes	the
T8955	24372-24379	NN	denotes	surface
T8956	24380-24382	IN	denotes	of
T8957	24383-24391	JJ	denotes	marginal
T8958	24392-24397	NNS	denotes	cells
T8959	24397-24398	.	denotes	.
T8960	24398-24584	sentence	denotes	Thickness of spiral prominence (SP) was measured perpendicular to a tangential line (dashed) that connects the surface of the outer sulcus (OS) with the basal layer of stria vascularis.
T8961	24399-24408	NN	denotes	Thickness
T8963	24409-24411	IN	denotes	of
T8964	24412-24418	JJ	denotes	spiral
T8965	24419-24429	NN	denotes	prominence
T8966	24430-24431	-LRB-	denotes	(
T8967	24431-24433	NN	denotes	SP
T8968	24433-24434	-RRB-	denotes	)
T8969	24435-24438	VBD	denotes	was
T8962	24439-24447	VBN	denotes	measured
T8970	24448-24461	JJ	denotes	perpendicular
T8971	24462-24464	IN	denotes	to
T8972	24465-24466	DT	denotes	a
T8974	24467-24477	JJ	denotes	tangential
T8973	24478-24482	NN	denotes	line
T8975	24483-24484	-LRB-	denotes	(
T8976	24484-24490	JJ	denotes	dashed
T8977	24490-24491	-RRB-	denotes	)
T8978	24492-24496	WDT	denotes	that
T8979	24497-24505	VBZ	denotes	connects
T8980	24506-24509	DT	denotes	the
T8981	24510-24517	NN	denotes	surface
T8982	24518-24520	IN	denotes	of
T8983	24521-24524	DT	denotes	the
T8985	24525-24530	JJ	denotes	outer
T8984	24531-24537	NN	denotes	sulcus
T8986	24538-24539	-LRB-	denotes	(
T8987	24539-24541	NN	denotes	OS
T8988	24541-24542	-RRB-	denotes	)
T8989	24543-24547	IN	denotes	with
T8990	24548-24551	DT	denotes	the
T8992	24552-24557	JJ	denotes	basal
T8991	24558-24563	NN	denotes	layer
T8993	24564-24566	IN	denotes	of
T8994	24567-24572	NN	denotes	stria
T8995	24573-24583	NN	denotes	vascularis
T8996	24583-24584	.	denotes	.
T8997	24584-24777	sentence	denotes	Thickness of spiral ligament (SL) was measured perpendicular to the tangential line as distance between the surface of spiral prominence and the interface between spiral ligament and bone (B).
T8998	24585-24594	NN	denotes	Thickness
T9000	24595-24597	IN	denotes	of
T9001	24598-24604	JJ	denotes	spiral
T9002	24605-24613	NN	denotes	ligament
T9003	24614-24615	-LRB-	denotes	(
T9004	24615-24617	NN	denotes	SL
T9005	24617-24618	-RRB-	denotes	)
T9006	24619-24622	VBD	denotes	was
T8999	24623-24631	VBN	denotes	measured
T9007	24632-24645	JJ	denotes	perpendicular
T9008	24646-24648	IN	denotes	to
T9009	24649-24652	DT	denotes	the
T9011	24653-24663	JJ	denotes	tangential
T9010	24664-24668	NN	denotes	line
T9012	24669-24671	IN	denotes	as
T9013	24672-24680	NN	denotes	distance
T9014	24681-24688	IN	denotes	between
T9015	24689-24692	DT	denotes	the
T9016	24693-24700	NN	denotes	surface
T9017	24701-24703	IN	denotes	of
T9018	24704-24710	JJ	denotes	spiral
T9019	24711-24721	NN	denotes	prominence
T9020	24722-24725	CC	denotes	and
T9021	24726-24729	DT	denotes	the
T9022	24730-24739	NN	denotes	interface
T9023	24740-24747	IN	denotes	between
T9024	24748-24754	JJ	denotes	spiral
T9025	24755-24763	NN	denotes	ligament
T9026	24764-24767	CC	denotes	and
T9027	24768-24772	NN	denotes	bone
T9028	24773-24774	-LRB-	denotes	(
T9029	24774-24775	NN	denotes	B
T9030	24775-24776	-RRB-	denotes	)
T9031	24776-24777	.	denotes	.
T9032	24777-25037	sentence	denotes	Thickness of spiral limbus (LIM) was obtained perpendicular to the surface of the bone as a tangential line that touches the inner sulcus (IS) and reaches from the surface of the spiral limbus to the interface between spiral limbus connective tissue and bone.
T9033	24778-24787	NN	denotes	Thickness
T9035	24788-24790	IN	denotes	of
T9036	24791-24797	JJ	denotes	spiral
T9037	24798-24804	NN	denotes	limbus
T9038	24805-24806	-LRB-	denotes	(
T9039	24806-24809	NN	denotes	LIM
T9040	24809-24810	-RRB-	denotes	)
T9041	24811-24814	VBD	denotes	was
T9034	24815-24823	VBN	denotes	obtained
T9042	24824-24837	JJ	denotes	perpendicular
T9043	24838-24840	IN	denotes	to
T9044	24841-24844	DT	denotes	the
T9045	24845-24852	NN	denotes	surface
T9046	24853-24855	IN	denotes	of
T9047	24856-24859	DT	denotes	the
T9048	24860-24864	NN	denotes	bone
T9049	24865-24867	IN	denotes	as
T9050	24868-24869	DT	denotes	a
T9052	24870-24880	JJ	denotes	tangential
T9051	24881-24885	NN	denotes	line
T9053	24886-24890	WDT	denotes	that
T9054	24891-24898	VBZ	denotes	touches
T9055	24899-24902	DT	denotes	the
T9057	24903-24908	JJ	denotes	inner
T9056	24909-24915	NN	denotes	sulcus
T9058	24916-24917	-LRB-	denotes	(
T9059	24917-24919	NN	denotes	IS
T9060	24919-24920	-RRB-	denotes	)
T9061	24921-24924	CC	denotes	and
T9062	24925-24932	VBZ	denotes	reaches
T9063	24933-24937	IN	denotes	from
T9064	24938-24941	DT	denotes	the
T9065	24942-24949	NN	denotes	surface
T9066	24950-24952	IN	denotes	of
T9067	24953-24956	DT	denotes	the
T9069	24957-24963	JJ	denotes	spiral
T9068	24964-24970	NN	denotes	limbus
T9070	24971-24973	IN	denotes	to
T9071	24974-24977	DT	denotes	the
T9072	24978-24987	NN	denotes	interface
T9073	24988-24995	IN	denotes	between
T9074	24996-25002	JJ	denotes	spiral
T9075	25003-25009	NN	denotes	limbus
T9076	25010-25020	JJ	denotes	connective
T9077	25021-25027	NN	denotes	tissue
T9078	25028-25031	CC	denotes	and
T9079	25032-25036	NN	denotes	bone
T9080	25036-25037	.	denotes	.
T9081	25037-25049	sentence	denotes	b: Summary.
T9082	25038-25039	LS	denotes	b
T9084	25039-25041	:	denotes	:
T9083	25041-25048	NN	denotes	Summary
T9085	25048-25049	.	denotes	.
T9086	25049-25099	sentence	denotes	Data from 7–8 animals contributed to each column.
T9087	25050-25054	NNS	denotes	Data
T9089	25055-25059	IN	denotes	from
T9090	25060-25061	CD	denotes	7
T9092	25061-25062	SYM	denotes	–
T9091	25062-25063	CD	denotes	8
T9093	25064-25071	NNS	denotes	animals
T9088	25072-25083	VBD	denotes	contributed
T9094	25084-25086	IN	denotes	to
T9095	25087-25091	DT	denotes	each
T9096	25092-25098	NN	denotes	column
T9097	25098-25099	.	denotes	.
T9176	25110-25118	NN	denotes	Analysis
T9177	25119-25121	IN	denotes	of
T9178	25122-25130	JJ	denotes	marginal
T9180	25131-25134	CC	denotes	and
T9181	25135-25140	JJ	denotes	basal
T9179	25141-25145	NN	denotes	cell
T9182	25146-25154	NNS	denotes	barriers
T9183	25155-25157	IN	denotes	by
T9184	25158-25160	IN	denotes	in
T9185	25161-25168	NN	denotes	Slc26a4
T9187	25168-25169	SYM	denotes	-
T9188	25169-25170	HYPH	denotes	/
T9189	25170-25171	SYM	denotes	-
T9186	25172-25176	NNS	denotes	mice
T9190	25176-25177	.	denotes	.
T9191	25177-25221	sentence	denotes	Tight junctions were visualized by F actin.
T9192	25178-25183	JJ	denotes	Tight
T9193	25184-25193	NNS	denotes	junctions
T9195	25194-25198	VBD	denotes	were
T9194	25199-25209	VBN	denotes	visualized
T9196	25210-25212	IN	denotes	by
T9197	25213-25214	NN	denotes	F
T9198	25215-25220	NN	denotes	actin
T9199	25220-25221	.	denotes	.
T9200	25221-25342	sentence	denotes	Whole-mounts of stria vascularis were viewed either from the basal cell side (a-f) or from the marginal cell side (g-l).
T9201	25222-25227	JJ	denotes	Whole
T9203	25227-25228	HYPH	denotes	-
T9202	25228-25234	NNS	denotes	mounts
T9205	25235-25237	IN	denotes	of
T9206	25238-25243	NN	denotes	stria
T9207	25244-25254	NN	denotes	vascularis
T9208	25255-25259	VBD	denotes	were
T9204	25260-25266	VBN	denotes	viewed
T9209	25267-25273	CC	denotes	either
T9210	25274-25278	IN	denotes	from
T9211	25279-25282	DT	denotes	the
T9213	25283-25288	JJ	denotes	basal
T9214	25289-25293	NN	denotes	cell
T9212	25294-25298	NN	denotes	side
T9215	25299-25300	-LRB-	denotes	(
T9216	25300-25301	NN	denotes	a
T9217	25301-25302	SYM	denotes	-
T9218	25302-25303	NN	denotes	f
T9219	25303-25304	-RRB-	denotes	)
T9220	25305-25307	CC	denotes	or
T9221	25308-25312	IN	denotes	from
T9222	25313-25316	DT	denotes	the
T9224	25317-25325	JJ	denotes	marginal
T9225	25326-25330	NN	denotes	cell
T9223	25331-25335	NN	denotes	side
T9226	25336-25337	-LRB-	denotes	(
T9227	25337-25338	NN	denotes	g
T9228	25338-25339	SYM	denotes	-
T9229	25339-25340	NN	denotes	l
T9230	25340-25341	-RRB-	denotes	)
T9231	25341-25342	.	denotes	.
T9232	25342-25427	sentence	denotes	Bright field images verify that the same area was viewed from either side (b and h).
T9233	25343-25349	JJ	denotes	Bright
T9234	25350-25355	NN	denotes	field
T9235	25356-25362	NNS	denotes	images
T9236	25363-25369	VBP	denotes	verify
T9237	25370-25374	IN	denotes	that
T9239	25375-25378	DT	denotes	the
T9241	25379-25383	JJ	denotes	same
T9240	25384-25388	NN	denotes	area
T9242	25389-25392	VBD	denotes	was
T9238	25393-25399	VBN	denotes	viewed
T9243	25400-25404	IN	denotes	from
T9244	25405-25411	CC	denotes	either
T9245	25412-25416	NN	denotes	side
T9246	25417-25418	-LRB-	denotes	(
T9247	25418-25419	NN	denotes	b
T9248	25420-25423	CC	denotes	and
T9249	25424-25425	NN	denotes	h
T9250	25425-25426	-RRB-	denotes	)
T9251	25426-25427	.	denotes	.
T9252	25427-25560	sentence	denotes	Colored bright field images were mixed with images of F actin staining to indicate the position of pigment granules (d, j, f and l).
T9253	25428-25435	JJ	denotes	Colored
T9255	25436-25442	JJ	denotes	bright
T9256	25443-25448	NN	denotes	field
T9254	25449-25455	NNS	denotes	images
T9258	25456-25460	VBD	denotes	were
T9257	25461-25466	VBN	denotes	mixed
T9259	25467-25471	IN	denotes	with
T9260	25472-25478	NNS	denotes	images
T9261	25479-25481	IN	denotes	of
T9262	25482-25483	NN	denotes	F
T9263	25484-25489	NN	denotes	actin
T9264	25490-25498	NN	denotes	staining
T9265	25499-25501	TO	denotes	to
T9266	25502-25510	VB	denotes	indicate
T9267	25511-25514	DT	denotes	the
T9268	25515-25523	NN	denotes	position
T9269	25524-25526	IN	denotes	of
T9270	25527-25534	NN	denotes	pigment
T9271	25535-25543	NNS	denotes	granules
T9272	25544-25545	-LRB-	denotes	(
T9273	25545-25546	NN	denotes	d
T9274	25546-25548	,	denotes	,
T9275	25548-25549	NN	denotes	j
T9276	25549-25551	,	denotes	,
T9277	25551-25552	NN	denotes	f
T9278	25553-25556	CC	denotes	and
T9279	25557-25558	NN	denotes	l
T9280	25558-25559	-RRB-	denotes	)
T9281	25559-25560	.	denotes	.
T9282	25560-25687	sentence	denotes	Focus was varied to either visualize the marginal cell barrier (SMC, c-d and i-j) or the basal cell barrier (BC, e-f and k-l).
T9283	25561-25566	NN	denotes	Focus
T9285	25567-25570	VBD	denotes	was
T9284	25571-25577	VBN	denotes	varied
T9286	25578-25580	TO	denotes	to
T9288	25581-25587	CC	denotes	either
T9287	25588-25597	VB	denotes	visualize
T9289	25598-25601	DT	denotes	the
T9291	25602-25610	JJ	denotes	marginal
T9292	25611-25615	NN	denotes	cell
T9290	25616-25623	NN	denotes	barrier
T9293	25624-25625	-LRB-	denotes	(
T9295	25625-25628	NN	denotes	SMC
T9296	25628-25630	,	denotes	,
T9294	25630-25631	NN	denotes	c
T9297	25631-25632	SYM	denotes	-
T9298	25632-25633	NN	denotes	d
T9299	25634-25637	CC	denotes	and
T9300	25638-25639	NN	denotes	i
T9301	25639-25640	SYM	denotes	-
T9302	25640-25641	NN	denotes	j
T9303	25641-25642	-RRB-	denotes	)
T9304	25643-25645	CC	denotes	or
T9305	25646-25649	DT	denotes	the
T9307	25650-25655	JJ	denotes	basal
T9308	25656-25660	NN	denotes	cell
T9306	25661-25668	NN	denotes	barrier
T9309	25669-25670	-LRB-	denotes	(
T9311	25670-25672	NN	denotes	BC
T9312	25672-25674	,	denotes	,
T9310	25674-25675	NN	denotes	e
T9313	25675-25676	SYM	denotes	-
T9314	25676-25677	NN	denotes	f
T9315	25678-25681	CC	denotes	and
T9316	25682-25683	NN	denotes	k
T9317	25683-25684	SYM	denotes	-
T9318	25684-25685	NN	denotes	l
T9319	25685-25686	-RRB-	denotes	)
T9320	25686-25687	.	denotes	.
T9321	25687-25772	sentence	denotes	Both the marginal cell (e-f) and the basal cell barrier (i-f) appeared to be intact.
T9322	25688-25692	CC	denotes	Both
T9324	25693-25696	DT	denotes	the
T9325	25697-25705	JJ	denotes	marginal
T9323	25706-25710	NN	denotes	cell
T9327	25711-25712	-LRB-	denotes	(
T9328	25712-25713	NN	denotes	e
T9329	25713-25714	SYM	denotes	-
T9330	25714-25715	NN	denotes	f
T9331	25715-25716	-RRB-	denotes	)
T9332	25717-25720	CC	denotes	and
T9333	25721-25724	DT	denotes	the
T9335	25725-25730	JJ	denotes	basal
T9334	25731-25735	NN	denotes	cell
T9336	25736-25743	NN	denotes	barrier
T9337	25744-25745	-LRB-	denotes	(
T9338	25745-25746	NN	denotes	i
T9339	25746-25747	SYM	denotes	-
T9340	25747-25748	NN	denotes	f
T9341	25748-25749	-RRB-	denotes	)
T9326	25750-25758	VBD	denotes	appeared
T9342	25759-25761	TO	denotes	to
T9343	25762-25764	VB	denotes	be
T9344	25765-25771	JJ	denotes	intact
T9345	25771-25772	.	denotes	.
T9346	25772-25860	sentence	denotes	It was critical for this finding that pigmentation did not block the path of the laser.
T9347	25773-25775	PRP	denotes	It
T9348	25776-25779	VBD	denotes	was
T9349	25780-25788	JJ	denotes	critical
T9350	25789-25792	IN	denotes	for
T9351	25793-25797	DT	denotes	this
T9352	25798-25805	NN	denotes	finding
T9353	25806-25810	IN	denotes	that
T9355	25811-25823	NN	denotes	pigmentation
T9356	25824-25827	VBD	denotes	did
T9357	25828-25831	RB	denotes	not
T9354	25832-25837	VB	denotes	block
T9358	25838-25841	DT	denotes	the
T9359	25842-25846	NN	denotes	path
T9360	25847-25849	IN	denotes	of
T9361	25850-25853	DT	denotes	the
T9362	25854-25859	NN	denotes	laser
T9363	25859-25860	.	denotes	.
T9364	25860-26005	sentence	denotes	Blockage of the laser by pigmentation produces the untrue impression of a discontinuous marginal cell barrier (c-d) or basal cell barrier (k-l).
T9365	25861-25869	NN	denotes	Blockage
T9367	25870-25872	IN	denotes	of
T9368	25873-25876	DT	denotes	the
T9369	25877-25882	NN	denotes	laser
T9370	25883-25885	IN	denotes	by
T9371	25886-25898	NN	denotes	pigmentation
T9366	25899-25907	VBZ	denotes	produces
T9372	25908-25911	DT	denotes	the
T9374	25912-25918	JJ	denotes	untrue
T9373	25919-25929	NN	denotes	impression
T9375	25930-25932	IN	denotes	of
T9376	25933-25934	DT	denotes	a
T9378	25935-25948	JJ	denotes	discontinuous
T9379	25949-25957	JJ	denotes	marginal
T9380	25958-25962	NN	denotes	cell
T9377	25963-25970	NN	denotes	barrier
T9381	25971-25972	-LRB-	denotes	(
T9382	25972-25973	NN	denotes	c
T9383	25973-25974	SYM	denotes	-
T9384	25974-25975	NN	denotes	d
T9385	25975-25976	-RRB-	denotes	)
T9386	25977-25979	CC	denotes	or
T9387	25980-25985	JJ	denotes	basal
T9388	25986-25990	NN	denotes	cell
T9389	25991-25998	NN	denotes	barrier
T9390	25999-26000	-LRB-	denotes	(
T9391	26000-26001	NN	denotes	k
T9392	26001-26002	SYM	denotes	-
T9393	26002-26003	NN	denotes	l
T9394	26003-26004	-RRB-	denotes	)
T9395	26004-26005	.	denotes	.
T9396	26005-26078	sentence	denotes	Comparison of images is aided by marking a significant area with a star.
T9397	26006-26016	NN	denotes	Comparison
T9399	26017-26019	IN	denotes	of
T9400	26020-26026	NNS	denotes	images
T9401	26027-26029	VBZ	denotes	is
T9398	26030-26035	VBN	denotes	aided
T9402	26036-26038	IN	denotes	by
T9403	26039-26046	VBG	denotes	marking
T9404	26047-26048	DT	denotes	a
T9406	26049-26060	JJ	denotes	significant
T9405	26061-26065	NN	denotes	area
T9407	26066-26070	IN	denotes	with
T9408	26071-26072	DT	denotes	a
T9409	26073-26077	NN	denotes	star
T9410	26077-26078	.	denotes	.
T9411	26078-26092	sentence	denotes	Bars = 10 μm.
T9412	26079-26083	NNS	denotes	Bars
T9414	26084-26085	SYM	denotes	=
T9415	26086-26088	CD	denotes	10
T9413	26089-26091	NNS	denotes	μm
T9416	26091-26092	.	denotes	.
T9536	26103-26111	NN	denotes	Analysis
T9537	26112-26114	IN	denotes	of
T9538	26115-26123	JJ	denotes	marginal
T9540	26124-26127	CC	denotes	and
T9541	26128-26133	JJ	denotes	basal
T9542	26134-26138	NN	denotes	cell
T9539	26139-26147	NNS	denotes	barriers
T9543	26148-26150	IN	denotes	in
T9544	26151-26158	NN	denotes	Slc26a4
T9546	26158-26159	SYM	denotes	+
T9547	26159-26160	HYPH	denotes	/
T9548	26160-26161	SYM	denotes	+
T9549	26162-26165	CC	denotes	and
T9550	26166-26173	NN	denotes	Slc26a4
T9551	26173-26174	SYM	denotes	-
T9552	26174-26175	HYPH	denotes	/
T9553	26175-26176	SYM	denotes	-
T9545	26177-26181	NNS	denotes	mice
T9554	26181-26182	.	denotes	.
T9555	26182-26262	sentence	denotes	Tight junctions were visualized by F actin in whole-mounts of stria vascularis.
T9556	26183-26188	JJ	denotes	Tight
T9557	26189-26198	NNS	denotes	junctions
T9559	26199-26203	VBD	denotes	were
T9558	26204-26214	VBN	denotes	visualized
T9560	26215-26217	IN	denotes	by
T9561	26218-26219	NN	denotes	F
T9562	26220-26225	NN	denotes	actin
T9563	26226-26228	IN	denotes	in
T9564	26229-26234	JJ	denotes	whole
T9566	26234-26235	HYPH	denotes	-
T9565	26235-26241	NNS	denotes	mounts
T9567	26242-26244	IN	denotes	of
T9568	26245-26250	NN	denotes	stria
T9569	26251-26261	NN	denotes	vascularis
T9570	26261-26262	.	denotes	.
T9571	26262-26332	sentence	denotes	Bright field images were taken to evaluate pigmentation (a, d and g).
T9572	26263-26269	JJ	denotes	Bright
T9573	26270-26275	NN	denotes	field
T9574	26276-26282	NNS	denotes	images
T9576	26283-26287	VBD	denotes	were
T9575	26288-26293	VBN	denotes	taken
T9577	26294-26296	TO	denotes	to
T9578	26297-26305	VB	denotes	evaluate
T9579	26306-26318	NN	denotes	pigmentation
T9580	26319-26320	-LRB-	denotes	(
T9581	26320-26321	NN	denotes	a
T9582	26321-26323	,	denotes	,
T9583	26323-26324	NN	denotes	d
T9584	26325-26328	CC	denotes	and
T9585	26329-26330	NN	denotes	g
T9586	26330-26331	-RRB-	denotes	)
T9587	26331-26332	.	denotes	.
T9588	26332-26414	sentence	denotes	Note the intact marginal cell (b) and basal cell (c) barriers in Slc26a4+/+ mice.
T9589	26333-26337	VB	denotes	Note
T9590	26338-26341	DT	denotes	the
T9592	26342-26348	JJ	denotes	intact
T9593	26349-26357	JJ	denotes	marginal
T9594	26358-26362	NN	denotes	cell
T9595	26363-26364	-LRB-	denotes	(
T9596	26364-26365	NN	denotes	b
T9597	26365-26366	-RRB-	denotes	)
T9598	26367-26370	CC	denotes	and
T9599	26371-26376	JJ	denotes	basal
T9600	26377-26381	NN	denotes	cell
T9601	26382-26383	-LRB-	denotes	(
T9602	26383-26384	NN	denotes	c
T9603	26384-26385	-RRB-	denotes	)
T9591	26386-26394	NNS	denotes	barriers
T9604	26395-26397	IN	denotes	in
T9605	26398-26405	NN	denotes	Slc26a4
T9607	26405-26406	SYM	denotes	+
T9608	26406-26407	HYPH	denotes	/
T9609	26407-26408	SYM	denotes	+
T9606	26409-26413	NNS	denotes	mice
T9610	26413-26414	.	denotes	.
T9611	26414-26495	sentence	denotes	Minimal pigmentation of Slc26a4+/+ mice did not compromise F actin localization.
T9612	26415-26422	JJ	denotes	Minimal
T9613	26423-26435	NN	denotes	pigmentation
T9615	26436-26438	IN	denotes	of
T9616	26439-26446	NN	denotes	Slc26a4
T9618	26446-26447	SYM	denotes	+
T9619	26447-26448	HYPH	denotes	/
T9620	26448-26449	SYM	denotes	+
T9617	26450-26454	NNS	denotes	mice
T9621	26455-26458	VBD	denotes	did
T9622	26459-26462	RB	denotes	not
T9614	26463-26473	VB	denotes	compromise
T9623	26474-26475	NN	denotes	F
T9624	26476-26481	NN	denotes	actin
T9625	26482-26494	NN	denotes	localization
T9626	26494-26495	.	denotes	.
T9627	26495-26637	sentence	denotes	Whole-mounts of stria vascularis from Slc26a4-/- mice were viewed either from the basal cell side (e-f) or from the marginal cell side (h-i).
T9628	26496-26501	JJ	denotes	Whole
T9630	26501-26502	HYPH	denotes	-
T9629	26502-26508	NNS	denotes	mounts
T9632	26509-26511	IN	denotes	of
T9633	26512-26517	NN	denotes	stria
T9634	26518-26528	NN	denotes	vascularis
T9635	26529-26533	IN	denotes	from
T9636	26534-26541	NN	denotes	Slc26a4
T9638	26541-26542	SYM	denotes	-
T9639	26542-26543	HYPH	denotes	/
T9640	26543-26544	SYM	denotes	-
T9637	26545-26549	NNS	denotes	mice
T9641	26550-26554	VBD	denotes	were
T9631	26555-26561	VBN	denotes	viewed
T9642	26562-26568	CC	denotes	either
T9643	26569-26573	IN	denotes	from
T9644	26574-26577	DT	denotes	the
T9646	26578-26583	JJ	denotes	basal
T9647	26584-26588	NN	denotes	cell
T9645	26589-26593	NN	denotes	side
T9648	26594-26595	-LRB-	denotes	(
T9649	26595-26596	NN	denotes	e
T9650	26596-26597	SYM	denotes	-
T9651	26597-26598	NN	denotes	f
T9652	26598-26599	-RRB-	denotes	)
T9653	26600-26602	CC	denotes	or
T9654	26603-26607	IN	denotes	from
T9655	26608-26611	DT	denotes	the
T9657	26612-26620	JJ	denotes	marginal
T9658	26621-26625	NN	denotes	cell
T9656	26626-26630	NN	denotes	side
T9659	26631-26632	-LRB-	denotes	(
T9660	26632-26633	NN	denotes	h
T9661	26633-26634	SYM	denotes	-
T9662	26634-26635	NN	denotes	i
T9663	26635-26636	-RRB-	denotes	)
T9664	26636-26637	.	denotes	.
T9665	26637-26722	sentence	denotes	Bright field images verify that the same area was viewed from either side (d and g).
T9666	26638-26644	JJ	denotes	Bright
T9667	26645-26650	NN	denotes	field
T9668	26651-26657	NNS	denotes	images
T9669	26658-26664	VBP	denotes	verify
T9670	26665-26669	IN	denotes	that
T9672	26670-26673	DT	denotes	the
T9674	26674-26678	JJ	denotes	same
T9673	26679-26683	NN	denotes	area
T9675	26684-26687	VBD	denotes	was
T9671	26688-26694	VBN	denotes	viewed
T9676	26695-26699	IN	denotes	from
T9677	26700-26706	CC	denotes	either
T9678	26707-26711	NN	denotes	side
T9679	26712-26713	-LRB-	denotes	(
T9680	26713-26714	NN	denotes	d
T9681	26715-26718	CC	denotes	and
T9682	26719-26720	NN	denotes	g
T9683	26720-26721	-RRB-	denotes	)
T9684	26721-26722	.	denotes	.
T9685	26722-26845	sentence	denotes	Focus was varied to either visualize the marginal cell barrier (SMC, b,e and h) or the basal cell barrier (BC, c,f and i).
T9686	26723-26728	NN	denotes	Focus
T9688	26729-26732	VBD	denotes	was
T9687	26733-26739	VBN	denotes	varied
T9689	26740-26742	TO	denotes	to
T9691	26743-26749	CC	denotes	either
T9690	26750-26759	VB	denotes	visualize
T9692	26760-26763	DT	denotes	the
T9694	26764-26772	JJ	denotes	marginal
T9695	26773-26777	NN	denotes	cell
T9693	26778-26785	NN	denotes	barrier
T9696	26786-26787	-LRB-	denotes	(
T9698	26787-26790	NN	denotes	SMC
T9699	26790-26792	,	denotes	,
T9697	26792-26793	NN	denotes	b
T9700	26793-26794	,	denotes	,
T9701	26794-26795	NN	denotes	e
T9702	26796-26799	CC	denotes	and
T9703	26800-26801	NN	denotes	h
T9704	26801-26802	-RRB-	denotes	)
T9705	26803-26805	CC	denotes	or
T9706	26806-26809	DT	denotes	the
T9708	26810-26815	JJ	denotes	basal
T9709	26816-26820	NN	denotes	cell
T9707	26821-26828	NN	denotes	barrier
T9710	26829-26830	-LRB-	denotes	(
T9712	26830-26832	NN	denotes	BC
T9713	26832-26834	,	denotes	,
T9711	26834-26835	NN	denotes	c
T9714	26835-26836	,	denotes	,
T9715	26836-26837	NN	denotes	f
T9716	26838-26841	CC	denotes	and
T9717	26842-26843	NN	denotes	i
T9718	26843-26844	-RRB-	denotes	)
T9719	26844-26845	.	denotes	.
T9720	26845-26927	sentence	denotes	Both the basal cell (f) and the marginal cell (h) barriers appeared to be intact.
T9721	26846-26850	CC	denotes	Both
T9723	26851-26854	DT	denotes	the
T9724	26855-26860	JJ	denotes	basal
T9722	26861-26865	NN	denotes	cell
T9726	26866-26867	-LRB-	denotes	(
T9727	26867-26868	NN	denotes	f
T9728	26868-26869	-RRB-	denotes	)
T9729	26870-26873	CC	denotes	and
T9730	26874-26877	DT	denotes	the
T9732	26878-26886	JJ	denotes	marginal
T9731	26887-26891	NN	denotes	cell
T9734	26892-26893	-LRB-	denotes	(
T9735	26893-26894	NN	denotes	h
T9736	26894-26895	-RRB-	denotes	)
T9733	26896-26904	NNS	denotes	barriers
T9725	26905-26913	VBD	denotes	appeared
T9737	26914-26916	TO	denotes	to
T9738	26917-26919	VB	denotes	be
T9739	26920-26926	JJ	denotes	intact
T9740	26926-26927	.	denotes	.
T9741	26927-27067	sentence	denotes	Blockage of the laser by pigmentation produces the untrue impression of 'holes' in the marginal cell barrier (e) or basal cell barrier (i).
T9742	26928-26936	NN	denotes	Blockage
T9744	26937-26939	IN	denotes	of
T9745	26940-26943	DT	denotes	the
T9746	26944-26949	NN	denotes	laser
T9747	26950-26952	IN	denotes	by
T9748	26953-26965	NN	denotes	pigmentation
T9743	26966-26974	VBZ	denotes	produces
T9749	26975-26978	DT	denotes	the
T9751	26979-26985	JJ	denotes	untrue
T9750	26986-26996	NN	denotes	impression
T9752	26997-26999	IN	denotes	of
T9753	27000-27001	``	denotes	'
T9754	27001-27006	NNS	denotes	holes
T9755	27006-27007	''	denotes	'
T9756	27008-27010	IN	denotes	in
T9757	27011-27014	DT	denotes	the
T9759	27015-27023	JJ	denotes	marginal
T9760	27024-27028	NN	denotes	cell
T9758	27029-27036	NN	denotes	barrier
T9761	27037-27038	-LRB-	denotes	(
T9762	27038-27039	NN	denotes	e
T9763	27039-27040	-RRB-	denotes	)
T9764	27041-27043	CC	denotes	or
T9765	27044-27049	JJ	denotes	basal
T9766	27050-27054	NN	denotes	cell
T9767	27055-27062	NN	denotes	barrier
T9768	27063-27064	-LRB-	denotes	(
T9769	27064-27065	NN	denotes	i
T9770	27065-27066	-RRB-	denotes	)
T9771	27066-27067	.	denotes	.
T9772	27067-27152	sentence	denotes	Comparison of images is aided by marking three significant areas with colored stars.
T9773	27068-27078	NN	denotes	Comparison
T9775	27079-27081	IN	denotes	of
T9776	27082-27088	NNS	denotes	images
T9777	27089-27091	VBZ	denotes	is
T9774	27092-27097	VBN	denotes	aided
T9778	27098-27100	IN	denotes	by
T9779	27101-27108	VBG	denotes	marking
T9780	27109-27114	CD	denotes	three
T9782	27115-27126	JJ	denotes	significant
T9781	27127-27132	NNS	denotes	areas
T9783	27133-27137	IN	denotes	with
T9784	27138-27145	JJ	denotes	colored
T9785	27146-27151	NNS	denotes	stars
T9786	27151-27152	.	denotes	.
T9787	27152-27166	sentence	denotes	Bars = 10 μm.
T9788	27153-27157	NNS	denotes	Bars
T9790	27158-27159	SYM	denotes	=
T9791	27160-27162	CD	denotes	10
T9789	27163-27165	NNS	denotes	μm
T9792	27165-27166	.	denotes	.
T6484	27167-27168	NN	denotes	P
T6482	27167-27174	sentence	denotes	Pendrin
T6483	27168-27169	VBN	denotes	e
T6485	27169-27172	JJ	denotes	ndr
T6487	27172-27173	JJ	denotes	i
T6486	27173-27174	NN	denotes	n
T6490	27174-27175	SYM	denotes	-
T6488	27174-27176	sentence	denotes	-e
T6489	27175-27176	NNS	denotes	e
T6492	27176-27177	VBN	denotes	x
T6491	27176-27181	sentence	denotes	xpres
T6493	27177-27178	NN	denotes	p
T6494	27178-27181	NNS	denotes	res
T6496	27181-27182	NN	denotes	s
T6495	27181-27184	sentence	denotes	sin
T6497	27182-27183	VBN	denotes	i
T6498	27183-27184	JJ	denotes	n
T6500	27184-27185	JJ	denotes	g
T6499	27184-27187	sentence	denotes	g s
T6501	27186-27187	JJ	denotes	s
T6503	27187-27188	VBZ	denotes	u
T6502	27187-27195	sentence	denotes	urface e
T6504	27188-27189	JJ	denotes	r
T6505	27189-27190	IN	denotes	f
T6506	27190-27191	DT	denotes	a
T6508	27191-27192	JJ	denotes	c
T6509	27192-27193	NN	denotes	e
T6507	27194-27195	NN	denotes	e
T6511	27195-27197	NN	denotes	pi
T6510	27195-27199	sentence	denotes	pith
T6512	27197-27198	JJ	denotes	t
T6513	27198-27199	IN	denotes	h
T6515	27199-27200	NN	denotes	e
T6514	27199-27200	sentence	denotes	e
T6517	27200-27202	NN	denotes	li
T6516	27200-27214	sentence	denotes	lial cells in
T6518	27202-27204	JJ	denotes	al
T6520	27205-27209	NN	denotes	cell
T6519	27209-27210	NNS	denotes	s
T6521	27211-27213	IN	denotes	in
T6523	27214-27215	JJ	denotes	t
T6522	27214-27219	sentence	denotes	the s
T6524	27215-27217	JJ	denotes	he
T6525	27218-27219	NN	denotes	s
T6527	27219-27221	NN	denotes	pi
T6526	27219-27221	sentence	denotes	pi
T6529	27221-27224	JJ	denotes	ral
T6528	27221-27225	sentence	denotes	ral
T6531	27225-27226	NN	denotes	p
T6530	27225-27231	sentence	denotes	promin
T6532	27226-27230	VB	denotes	romi
T6533	27230-27231	NN	denotes	n
T6535	27231-27232	JJ	denotes	e
T6534	27231-27240	sentence	denotes	ence regi
T6536	27232-27233	NNS	denotes	n
T6537	27233-27234	NN	denotes	c
T6538	27234-27235	NNS	denotes	e
T6539	27236-27238	VBD	denotes	re
T6540	27238-27240	JJ	denotes	gi
T6542	27240-27241	IN	denotes	o
T6541	27240-27243	sentence	denotes	on
T6543	27241-27242	CC	denotes	n
T6546	27243-27244	VBD	denotes	a
T6544	27243-27251	sentence	denotes	are loca
T6545	27244-27246	VBN	denotes	re
T6547	27247-27248	VB	denotes	l
T6548	27248-27249	CC	denotes	o
T6550	27249-27250	NN	denotes	c
T6549	27250-27251	NN	denotes	a
T6552	27251-27252	CC	denotes	t
T6551	27251-27260	sentence	denotes	ted in an
T6553	27252-27253	DT	denotes	e
T6554	27253-27254	CC	denotes	d
T6555	27255-27257	JJ	denotes	in
T6556	27258-27259	NNS	denotes	a
T6557	27259-27260	JJ	denotes	n
T6558	27260-27266	sentence	denotes	area
T6559	27261-27262	NN	denotes	a
T6560	27262-27263	NN	denotes	r
T6561	27263-27265	NN	denotes	ea
T6563	27266-27268	IN	denotes	wh
T6562	27266-27270	sentence	denotes	wher
T6564	27268-27269	JJ	denotes	e
T6565	27269-27270	NNS	denotes	r
T6567	27270-27271	NN	denotes	e
T6566	27270-27397	sentence	denotes	e basal cells, which are interconnected by tight junctions, form additional tight junctions with surface epithelial cells [29].
T6569	27272-27277	JJ	denotes	basal
T6570	27278-27283	NNS	denotes	cells
T6571	27283-27285	,	denotes	,
T6572	27285-27290	WDT	denotes	which
T6573	27291-27294	VBP	denotes	are
T6574	27295-27309	VBN	denotes	interconnected
T6575	27310-27312	IN	denotes	by
T6576	27313-27318	JJ	denotes	tight
T6577	27319-27328	NNS	denotes	junctions
T6578	27328-27330	,	denotes	,
T6568	27330-27334	VBP	denotes	form
T6579	27335-27345	JJ	denotes	additional
T6581	27346-27351	JJ	denotes	tight
T6580	27352-27361	NNS	denotes	junctions
T6582	27362-27366	IN	denotes	with
T6583	27367-27374	NN	denotes	surface
T6585	27375-27385	JJ	denotes	epithelial
T6584	27386-27391	NNS	denotes	cells
T6586	27392-27393	-LRB-	denotes	[
T6587	27393-27395	CD	denotes	29
T6588	27395-27396	-RRB-	denotes	]
T6589	27396-27397	.	denotes	.
T6590	27397-27514	sentence	denotes	A discontinuity of this complex junction in Slc26a4-/- mice would explain the absence of the endocochlear potential.
T6591	27398-27399	DT	denotes	A
T6592	27400-27413	NN	denotes	discontinuity
T6594	27414-27416	IN	denotes	of
T6595	27417-27421	DT	denotes	this
T6597	27422-27429	JJ	denotes	complex
T6596	27430-27438	NN	denotes	junction
T6598	27439-27441	IN	denotes	in
T6599	27442-27449	NN	denotes	Slc26a4
T6601	27449-27450	SYM	denotes	-
T6602	27450-27451	HYPH	denotes	/
T6603	27451-27452	SYM	denotes	-
T6600	27453-27457	NNS	denotes	mice
T6604	27458-27463	MD	denotes	would
T6593	27464-27471	VB	denotes	explain
T6605	27472-27475	DT	denotes	the
T6606	27476-27483	NN	denotes	absence
T6607	27484-27486	IN	denotes	of
T6608	27487-27490	DT	denotes	the
T6610	27491-27503	JJ	denotes	endocochlear
T6609	27504-27513	NN	denotes	potential
T6611	27513-27514	.	denotes	.
T6612	27514-27691	sentence	denotes	To evaluate the presence of this connection, we determined by confocal immunocytochemistry the expression of ZO-1 and of F-actin, which associate with tight junction complexes.
T6613	27515-27517	TO	denotes	To
T6614	27518-27526	VB	denotes	evaluate
T6616	27527-27530	DT	denotes	the
T6617	27531-27539	NN	denotes	presence
T6618	27540-27542	IN	denotes	of
T6619	27543-27547	DT	denotes	this
T6620	27548-27558	NN	denotes	connection
T6621	27558-27560	,	denotes	,
T6622	27560-27562	PRP	denotes	we
T6615	27563-27573	VBD	denotes	determined
T6623	27574-27576	IN	denotes	by
T6624	27577-27585	JJ	denotes	confocal
T6625	27586-27605	NN	denotes	immunocytochemistry
T6626	27606-27609	DT	denotes	the
T6627	27610-27620	NN	denotes	expression
T6628	27621-27623	IN	denotes	of
T6629	27624-27626	NN	denotes	ZO
T6630	27626-27627	HYPH	denotes	-
T6631	27627-27628	CD	denotes	1
T6632	27629-27632	CC	denotes	and
T6633	27633-27635	IN	denotes	of
T6634	27636-27637	NN	denotes	F
T6636	27637-27638	HYPH	denotes	-
T6635	27638-27643	NN	denotes	actin
T6637	27643-27645	,	denotes	,
T6638	27645-27650	WDT	denotes	which
T6639	27651-27660	VBP	denotes	associate
T6640	27661-27665	IN	denotes	with
T6641	27666-27671	JJ	denotes	tight
T6643	27672-27680	NN	denotes	junction
T6642	27681-27690	NNS	denotes	complexes
T6644	27690-27691	.	denotes	.
T6645	27691-27900	sentence	denotes	ZO-1 and F-actin expression revealed a continuous layer of basal cells, including a junction of basal cells with surface epithelial cells in Slc26a4-/- mice, as observed in normal mice (Fig. 1c,1d,1e,1f,6,7).
T6646	27692-27694	NN	denotes	ZO
T6648	27694-27695	HYPH	denotes	-
T6649	27695-27696	CD	denotes	1
T6650	27697-27700	CC	denotes	and
T6651	27701-27702	NN	denotes	F
T6653	27702-27703	HYPH	denotes	-
T6652	27703-27708	NN	denotes	actin
T6647	27709-27719	NN	denotes	expression
T6654	27720-27728	VBD	denotes	revealed
T6655	27729-27730	DT	denotes	a
T6657	27731-27741	JJ	denotes	continuous
T6656	27742-27747	NN	denotes	layer
T6658	27748-27750	IN	denotes	of
T6659	27751-27756	JJ	denotes	basal
T6660	27757-27762	NNS	denotes	cells
T6661	27762-27764	,	denotes	,
T6662	27764-27773	VBG	denotes	including
T6663	27774-27775	DT	denotes	a
T6664	27776-27784	NN	denotes	junction
T6665	27785-27787	IN	denotes	of
T6666	27788-27793	JJ	denotes	basal
T6667	27794-27799	NNS	denotes	cells
T6668	27800-27804	IN	denotes	with
T6669	27805-27812	NN	denotes	surface
T6671	27813-27823	JJ	denotes	epithelial
T6670	27824-27829	NNS	denotes	cells
T6672	27830-27832	IN	denotes	in
T6673	27833-27840	NN	denotes	Slc26a4
T6675	27840-27841	SYM	denotes	-
T6676	27841-27842	HYPH	denotes	/
T6677	27842-27843	SYM	denotes	-
T6674	27844-27848	NNS	denotes	mice
T6678	27848-27850	,	denotes	,
T6679	27850-27852	IN	denotes	as
T6680	27853-27861	VBN	denotes	observed
T6681	27862-27864	IN	denotes	in
T6682	27865-27871	JJ	denotes	normal
T6683	27872-27876	NNS	denotes	mice
T6684	27877-27878	-LRB-	denotes	(
T6686	27878-27882	NN	denotes	Fig.
T6688	27883-27885	CD	denotes	1c
T6689	27885-27886	,	denotes	,
T6690	27886-27888	CD	denotes	1d
T6691	27888-27889	,	denotes	,
T6692	27889-27891	CD	denotes	1e
T6693	27891-27892	,	denotes	,
T6687	27892-27894	CD	denotes	1f
T6694	27894-27895	,	denotes	,
T6695	27895-27896	CD	denotes	6
T6696	27896-27897	,	denotes	,
T6685	27897-27898	CD	denotes	7
T6697	27898-27899	-RRB-	denotes	)
T6698	27899-27900	.	denotes	.
T6699	27900-28044	sentence	denotes	These observations make it unlikely that the primary cause of the loss of the endocochlear potential is a compromise in the basal cell barrier.
T6700	27901-27906	DT	denotes	These
T6701	27907-27919	NNS	denotes	observations
T6702	27920-27924	VBP	denotes	make
T6703	27925-27927	PRP	denotes	it
T6704	27928-27936	JJ	denotes	unlikely
T6705	27937-27941	IN	denotes	that
T6707	27942-27945	DT	denotes	the
T6709	27946-27953	JJ	denotes	primary
T6708	27954-27959	NN	denotes	cause
T6710	27960-27962	IN	denotes	of
T6711	27963-27966	DT	denotes	the
T6712	27967-27971	NN	denotes	loss
T6713	27972-27974	IN	denotes	of
T6714	27975-27978	DT	denotes	the
T6716	27979-27991	JJ	denotes	endocochlear
T6715	27992-28001	NN	denotes	potential
T6706	28002-28004	VBZ	denotes	is
T6717	28005-28006	DT	denotes	a
T6718	28007-28017	NN	denotes	compromise
T6719	28018-28020	IN	denotes	in
T6720	28021-28024	DT	denotes	the
T6722	28025-28030	JJ	denotes	basal
T6723	28031-28035	NN	denotes	cell
T6721	28036-28043	NN	denotes	barrier
T6724	28043-28044	.	denotes	.
T6725	28044-28248	sentence	denotes	The observation that endolymphatic and perilymphatic K+ concentrations were normal in Slc26a4-/- mice suggests that stria vascularis was able to secrete K+ in spite of the apparent signs of degeneration.
T6726	28045-28048	DT	denotes	The
T6727	28049-28060	NN	denotes	observation
T6729	28061-28065	IN	denotes	that
T6731	28066-28079	JJ	denotes	endolymphatic
T6733	28080-28083	CC	denotes	and
T6734	28084-28097	JJ	denotes	perilymphatic
T6735	28098-28099	NN	denotes	K
T6736	28099-28100	SYM	denotes	+
T6732	28101-28115	NNS	denotes	concentrations
T6730	28116-28120	VBD	denotes	were
T6737	28121-28127	JJ	denotes	normal
T6738	28128-28130	IN	denotes	in
T6739	28131-28138	NN	denotes	Slc26a4
T6741	28138-28139	SYM	denotes	-
T6742	28139-28140	HYPH	denotes	/
T6743	28140-28141	SYM	denotes	-
T6740	28142-28146	NNS	denotes	mice
T6728	28147-28155	VBZ	denotes	suggests
T6744	28156-28160	IN	denotes	that
T6746	28161-28166	NN	denotes	stria
T6747	28167-28177	NN	denotes	vascularis
T6745	28178-28181	VBD	denotes	was
T6748	28182-28186	JJ	denotes	able
T6749	28187-28189	TO	denotes	to
T6750	28190-28197	VB	denotes	secrete
T6751	28198-28199	NN	denotes	K
T6752	28199-28200	SYM	denotes	+
T6753	28201-28203	IN	denotes	in
T6754	28204-28209	NN	denotes	spite
T6755	28210-28212	IN	denotes	of
T6756	28213-28216	DT	denotes	the
T6758	28217-28225	JJ	denotes	apparent
T6757	28226-28231	NNS	denotes	signs
T6759	28232-28234	IN	denotes	of
T6760	28235-28247	NN	denotes	degeneration
T6761	28247-28248	.	denotes	.
T6762	28248-28497	sentence	denotes	The rate of K+ secretion necessary to maintain endolymphatic K+ concentration, however, may be less than necessary in Slc26a4+/+ mice given the reduced numbers of sensory hair cells, which provide a major pathway for K+ exit from endolymph [30,31].
T6763	28249-28252	DT	denotes	The
T6764	28253-28257	NN	denotes	rate
T6766	28258-28260	IN	denotes	of
T6767	28261-28262	NN	denotes	K
T6769	28262-28263	SYM	denotes	+
T6768	28264-28273	NN	denotes	secretion
T6770	28274-28283	JJ	denotes	necessary
T6771	28284-28286	TO	denotes	to
T6772	28287-28295	VB	denotes	maintain
T6773	28296-28309	JJ	denotes	endolymphatic
T6775	28310-28311	NN	denotes	K
T6776	28311-28312	SYM	denotes	+
T6774	28313-28326	NN	denotes	concentration
T6777	28326-28328	,	denotes	,
T6778	28328-28335	RB	denotes	however
T6779	28335-28337	,	denotes	,
T6780	28337-28340	MD	denotes	may
T6765	28341-28343	VB	denotes	be
T6781	28344-28348	JJR	denotes	less
T6782	28349-28353	IN	denotes	than
T6783	28354-28363	JJ	denotes	necessary
T6784	28364-28366	IN	denotes	in
T6785	28367-28374	NN	denotes	Slc26a4
T6787	28374-28375	SYM	denotes	+
T6788	28375-28376	HYPH	denotes	/
T6789	28376-28377	SYM	denotes	+
T6786	28378-28382	NNS	denotes	mice
T6790	28383-28388	VBN	denotes	given
T6791	28389-28392	DT	denotes	the
T6793	28393-28400	JJ	denotes	reduced
T6792	28401-28408	NNS	denotes	numbers
T6794	28409-28411	IN	denotes	of
T6795	28412-28419	JJ	denotes	sensory
T6797	28420-28424	NN	denotes	hair
T6796	28425-28430	NNS	denotes	cells
T6798	28430-28432	,	denotes	,
T6799	28432-28437	WDT	denotes	which
T6800	28438-28445	VBP	denotes	provide
T6801	28446-28447	DT	denotes	a
T6803	28448-28453	JJ	denotes	major
T6802	28454-28461	NN	denotes	pathway
T6804	28462-28465	IN	denotes	for
T6805	28466-28467	NN	denotes	K
T6807	28467-28468	SYM	denotes	+
T6806	28469-28473	NN	denotes	exit
T6808	28474-28478	IN	denotes	from
T6809	28479-28488	NN	denotes	endolymph
T6810	28489-28490	-LRB-	denotes	[
T6812	28490-28492	CD	denotes	30
T6813	28492-28493	,	denotes	,
T6811	28493-28495	CD	denotes	31
T6814	28495-28496	-RRB-	denotes	]
T6815	28496-28497	.	denotes	.
T6816	28497-28911	sentence	denotes	In order to substantiate the view that stria vascularis in Slc26a4-/- secretes K+, we measured the magnitude of the bumetanide-sensitive current exiting stria vascularis across the apical membrane of strial marginal cells and determined the expression of the proteins KCNQ1, KCNE1 and SLC12A2, which are essential for K+ secretion [20,32,33], and of GJB2 (Cx26), which is thought to contribute to K+ cycling [23].
T6817	28498-28500	IN	denotes	In
T6819	28501-28506	NN	denotes	order
T6820	28507-28509	TO	denotes	to
T6821	28510-28522	VB	denotes	substantiate
T6822	28523-28526	DT	denotes	the
T6823	28527-28531	NN	denotes	view
T6824	28532-28536	IN	denotes	that
T6826	28537-28542	NN	denotes	stria
T6827	28543-28553	NN	denotes	vascularis
T6828	28554-28556	IN	denotes	in
T6829	28557-28564	NN	denotes	Slc26a4
T6830	28564-28565	SYM	denotes	-
T6831	28565-28566	HYPH	denotes	/
T6832	28566-28567	SYM	denotes	-
T6825	28568-28576	VBZ	denotes	secretes
T6833	28577-28578	NN	denotes	K
T6834	28578-28579	SYM	denotes	+
T6835	28579-28581	,	denotes	,
T6836	28581-28583	PRP	denotes	we
T6818	28584-28592	VBD	denotes	measured
T6837	28593-28596	DT	denotes	the
T6838	28597-28606	NN	denotes	magnitude
T6839	28607-28609	IN	denotes	of
T6840	28610-28613	DT	denotes	the
T6842	28614-28624	NN	denotes	bumetanide
T6844	28624-28625	HYPH	denotes	-
T6843	28625-28634	JJ	denotes	sensitive
T6841	28635-28642	NN	denotes	current
T6845	28643-28650	VBG	denotes	exiting
T6846	28651-28656	NN	denotes	stria
T6847	28657-28667	NN	denotes	vascularis
T6848	28668-28674	IN	denotes	across
T6849	28675-28678	DT	denotes	the
T6851	28679-28685	JJ	denotes	apical
T6850	28686-28694	NN	denotes	membrane
T6852	28695-28697	IN	denotes	of
T6853	28698-28704	JJ	denotes	strial
T6855	28705-28713	JJ	denotes	marginal
T6854	28714-28719	NNS	denotes	cells
T6856	28720-28723	CC	denotes	and
T6857	28724-28734	VBD	denotes	determined
T6858	28735-28738	DT	denotes	the
T6859	28739-28749	NN	denotes	expression
T6860	28750-28752	IN	denotes	of
T6861	28753-28756	DT	denotes	the
T6862	28757-28765	NN	denotes	proteins
T6863	28766-28771	NN	denotes	KCNQ1
T6864	28771-28773	,	denotes	,
T6865	28773-28778	NN	denotes	KCNE1
T6866	28779-28782	CC	denotes	and
T6867	28783-28790	NN	denotes	SLC12A2
T6868	28790-28792	,	denotes	,
T6869	28792-28797	WDT	denotes	which
T6870	28798-28801	VBP	denotes	are
T6871	28802-28811	JJ	denotes	essential
T6872	28812-28815	IN	denotes	for
T6873	28816-28817	NN	denotes	K
T6875	28817-28818	SYM	denotes	+
T6874	28819-28828	NN	denotes	secretion
T6876	28829-28830	-LRB-	denotes	[
T6878	28830-28832	CD	denotes	20
T6879	28832-28833	,	denotes	,
T6880	28833-28835	CD	denotes	32
T6881	28835-28836	,	denotes	,
T6877	28836-28838	CD	denotes	33
T6882	28838-28839	-RRB-	denotes	]
T6883	28839-28841	,	denotes	,
T6884	28841-28844	CC	denotes	and
T6885	28845-28847	IN	denotes	of
T6886	28848-28852	NN	denotes	GJB2
T6888	28853-28854	-LRB-	denotes	(
T6887	28854-28858	NN	denotes	Cx26
T6889	28858-28859	-RRB-	denotes	)
T6890	28859-28861	,	denotes	,
T6891	28861-28866	WDT	denotes	which
T6893	28867-28869	VBZ	denotes	is
T6892	28870-28877	VBN	denotes	thought
T6894	28878-28880	TO	denotes	to
T6895	28881-28891	VB	denotes	contribute
T6896	28892-28894	IN	denotes	to
T6897	28895-28896	NN	denotes	K
T6899	28896-28897	SYM	denotes	+
T6898	28898-28905	NN	denotes	cycling
T6900	28906-28907	-LRB-	denotes	[
T6901	28907-28909	CD	denotes	23
T6902	28909-28910	-RRB-	denotes	]
T6903	28910-28911	.	denotes	.
T6904	28911-29045	sentence	denotes	K+ secretion is known to be sensitive to 10-5 M bumetanide, a loop-diuretic that inhibits the Na+/2Cl-/K+ cotransporter SLC12A2 [26].
T6905	28912-28913	NN	denotes	K
T6907	28913-28914	SYM	denotes	+
T6906	28915-28924	NN	denotes	secretion
T6909	28925-28927	VBZ	denotes	is
T6908	28928-28933	VBN	denotes	known
T6910	28934-28936	TO	denotes	to
T6911	28937-28939	VB	denotes	be
T6912	28940-28949	JJ	denotes	sensitive
T6913	28950-28952	IN	denotes	to
T6914	28953-28955	CD	denotes	10
T6916	28955-28956	HYPH	denotes	-
T6915	28956-28957	CD	denotes	5
T6917	28958-28959	NN	denotes	M
T6918	28960-28970	NN	denotes	bumetanide
T6919	28970-28972	,	denotes	,
T6920	28972-28973	DT	denotes	a
T6922	28974-28978	NN	denotes	loop
T6923	28978-28979	HYPH	denotes	-
T6921	28979-28987	NN	denotes	diuretic
T6924	28988-28992	WDT	denotes	that
T6925	28993-29001	VBZ	denotes	inhibits
T6926	29002-29005	DT	denotes	the
T6928	29006-29008	NN	denotes	Na
T6929	29008-29009	SYM	denotes	+
T6930	29009-29010	HYPH	denotes	/
T6931	29010-29013	NN	denotes	2Cl
T6932	29013-29014	SYM	denotes	-
T6933	29014-29015	HYPH	denotes	/
T6934	29015-29016	NN	denotes	K
T6935	29016-29017	SYM	denotes	+
T6927	29018-29031	NN	denotes	cotransporter
T6936	29032-29039	NN	denotes	SLC12A2
T6937	29040-29041	-LRB-	denotes	[
T6938	29041-29043	CD	denotes	26
T6939	29043-29044	-RRB-	denotes	]
T6940	29044-29045	.	denotes	.
T6941	29045-29260	sentence	denotes	Bumetanide-sensitive currents were found in both Slc26a4+/+ and Slc26a4-/- mice although the magnitude of the current was significantly smaller in Slc26a4-/- mice (22 ± 6 μA/cm2, n = 4 versus 14 ± 2 μA/cm2, n = 4).
T6942	29046-29056	NN	denotes	Bumetanide
T6944	29056-29057	HYPH	denotes	-
T6943	29057-29066	JJ	denotes	sensitive
T6945	29067-29075	NNS	denotes	currents
T6947	29076-29080	VBD	denotes	were
T6946	29081-29086	VBN	denotes	found
T6948	29087-29089	IN	denotes	in
T6949	29090-29094	CC	denotes	both
T6950	29095-29102	NN	denotes	Slc26a4
T6952	29102-29103	SYM	denotes	+
T6953	29103-29104	HYPH	denotes	/
T6954	29104-29105	SYM	denotes	+
T6955	29106-29109	CC	denotes	and
T6956	29110-29117	NN	denotes	Slc26a4
T6957	29117-29118	SYM	denotes	-
T6958	29118-29119	HYPH	denotes	/
T6959	29119-29120	SYM	denotes	-
T6951	29121-29125	NNS	denotes	mice
T6960	29126-29134	IN	denotes	although
T6962	29135-29138	DT	denotes	the
T6963	29139-29148	NN	denotes	magnitude
T6964	29149-29151	IN	denotes	of
T6965	29152-29155	DT	denotes	the
T6966	29156-29163	NN	denotes	current
T6961	29164-29167	VBD	denotes	was
T6967	29168-29181	RB	denotes	significantly
T6968	29182-29189	JJR	denotes	smaller
T6969	29190-29192	IN	denotes	in
T6970	29193-29200	NN	denotes	Slc26a4
T6972	29200-29201	SYM	denotes	-
T6973	29201-29202	HYPH	denotes	/
T6974	29202-29203	SYM	denotes	-
T6971	29204-29208	NNS	denotes	mice
T6975	29209-29210	-LRB-	denotes	(
T6977	29210-29212	CD	denotes	22
T6979	29213-29214	SYM	denotes	±
T6978	29215-29216	CD	denotes	6
T6980	29217-29219	NN	denotes	μA
T6981	29219-29220	SYM	denotes	/
T6982	29220-29223	NN	denotes	cm2
T6983	29223-29225	,	denotes	,
T6984	29225-29226	NN	denotes	n
T6985	29227-29228	SYM	denotes	=
T6976	29229-29230	CD	denotes	4
T6986	29231-29237	CC	denotes	versus
T6987	29238-29240	CD	denotes	14
T6989	29241-29242	SYM	denotes	±
T6988	29243-29244	CD	denotes	2
T6990	29245-29247	NNS	denotes	μA
T6992	29247-29248	SYM	denotes	/
T6993	29248-29251	NN	denotes	cm2
T6994	29251-29253	,	denotes	,
T6995	29253-29254	NN	denotes	n
T6996	29255-29256	SYM	denotes	=
T6991	29257-29258	CD	denotes	4
T6997	29258-29259	-RRB-	denotes	)
T6998	29259-29260	.	denotes	.
T6999	29260-29630	sentence	denotes	KCNQ1 and KCNE1, subunits of the secretory K+ channel, were co-localized in the apical membrane of strial marginal cells; the Na+/2Cl-/K+ cotransporter SLC12A2, which is located in the basolateral membrane of strial marginal cells, was found in stria vascularis; and the gap junction protein GJB2 was found in spiral ligament of Slc26a4+/+ and Slc26a4-/- mice (Fig. 8).
T7000	29261-29266	NN	denotes	KCNQ1
T7002	29267-29270	CC	denotes	and
T7003	29271-29276	NN	denotes	KCNE1
T7004	29276-29278	,	denotes	,
T7005	29278-29286	NNS	denotes	subunits
T7006	29287-29289	IN	denotes	of
T7007	29290-29293	DT	denotes	the
T7009	29294-29303	JJ	denotes	secretory
T7010	29304-29305	NN	denotes	K
T7011	29305-29306	SYM	denotes	+
T7008	29307-29314	NN	denotes	channel
T7012	29314-29316	,	denotes	,
T7013	29316-29320	VBD	denotes	were
T7001	29321-29333	VBN	denotes	co-localized
T7014	29334-29336	IN	denotes	in
T7015	29337-29340	DT	denotes	the
T7017	29341-29347	JJ	denotes	apical
T7016	29348-29356	NN	denotes	membrane
T7018	29357-29359	IN	denotes	of
T7019	29360-29366	JJ	denotes	strial
T7020	29367-29375	JJ	denotes	marginal
T7021	29376-29381	NNS	denotes	cells
T7022	29381-29382	:	denotes	;
T7023	29383-29386	DT	denotes	the
T7025	29387-29389	NN	denotes	Na
T7026	29389-29390	SYM	denotes	+
T7027	29390-29391	HYPH	denotes	/
T7028	29391-29394	NN	denotes	2Cl
T7029	29394-29395	SYM	denotes	-
T7030	29395-29396	HYPH	denotes	/
T7031	29396-29397	NN	denotes	K
T7032	29397-29398	SYM	denotes	+
T7024	29399-29412	NN	denotes	cotransporter
T7034	29413-29420	NN	denotes	SLC12A2
T7035	29420-29422	,	denotes	,
T7036	29422-29427	WDT	denotes	which
T7038	29428-29430	VBZ	denotes	is
T7037	29431-29438	VBN	denotes	located
T7039	29439-29441	IN	denotes	in
T7040	29442-29445	DT	denotes	the
T7042	29446-29457	JJ	denotes	basolateral
T7041	29458-29466	NN	denotes	membrane
T7043	29467-29469	IN	denotes	of
T7044	29470-29476	JJ	denotes	strial
T7046	29477-29485	JJ	denotes	marginal
T7045	29486-29491	NNS	denotes	cells
T7047	29491-29493	,	denotes	,
T7048	29493-29496	VBD	denotes	was
T7033	29497-29502	VBN	denotes	found
T7049	29503-29505	IN	denotes	in
T7050	29506-29511	NN	denotes	stria
T7051	29512-29522	NN	denotes	vascularis
T7052	29522-29523	:	denotes	;
T7053	29524-29527	CC	denotes	and
T7054	29528-29531	DT	denotes	the
T7056	29532-29535	NN	denotes	gap
T7057	29536-29544	NN	denotes	junction
T7055	29545-29552	NN	denotes	protein
T7059	29553-29557	NN	denotes	GJB2
T7060	29558-29561	VBD	denotes	was
T7058	29562-29567	VBN	denotes	found
T7061	29568-29570	IN	denotes	in
T7062	29571-29577	JJ	denotes	spiral
T7063	29578-29586	NN	denotes	ligament
T7064	29587-29589	IN	denotes	of
T7065	29590-29597	NN	denotes	Slc26a4
T7067	29597-29598	SYM	denotes	+
T7068	29598-29599	HYPH	denotes	/
T7069	29599-29600	SYM	denotes	+
T7070	29601-29604	CC	denotes	and
T7071	29605-29612	NN	denotes	Slc26a4
T7072	29612-29613	SYM	denotes	-
T7073	29613-29614	HYPH	denotes	/
T7074	29614-29615	SYM	denotes	-
T7066	29616-29620	NNS	denotes	mice
T7075	29621-29622	-LRB-	denotes	(
T7076	29622-29626	NN	denotes	Fig.
T7077	29627-29628	CD	denotes	8
T7078	29628-29629	-RRB-	denotes	)
T7079	29629-29630	.	denotes	.
T7080	29630-29728	sentence	denotes	Co-localization of KCNQ1 and KCNE1 proteins was also observed in vestibular dark cells (Fig. 1i).
T7081	29631-29646	NN	denotes	Co-localization
T7083	29647-29649	IN	denotes	of
T7084	29650-29655	NN	denotes	KCNQ1
T7086	29656-29659	CC	denotes	and
T7087	29660-29665	NN	denotes	KCNE1
T7085	29666-29674	NN	denotes	proteins
T7088	29675-29678	VBD	denotes	was
T7089	29679-29683	RB	denotes	also
T7082	29684-29692	VBN	denotes	observed
T7090	29693-29695	IN	denotes	in
T7091	29696-29706	JJ	denotes	vestibular
T7093	29707-29711	JJ	denotes	dark
T7092	29712-29717	NNS	denotes	cells
T7094	29718-29719	-LRB-	denotes	(
T7095	29719-29723	NN	denotes	Fig.
T7096	29724-29726	CD	denotes	1i
T7097	29726-29727	-RRB-	denotes	)
T7098	29727-29728	.	denotes	.
T7099	29728-29861	sentence	denotes	Expression of KCNQ1 protein in stria vascularis of Slc26a4-/- mice is consistent with the finding of KCNQ1 mRNA expression (Fig. 3).
T7100	29729-29739	NN	denotes	Expression
T7102	29740-29742	IN	denotes	of
T7103	29743-29748	NN	denotes	KCNQ1
T7104	29749-29756	NN	denotes	protein
T7105	29757-29759	IN	denotes	in
T7106	29760-29765	NN	denotes	stria
T7107	29766-29776	NN	denotes	vascularis
T7108	29777-29779	IN	denotes	of
T7109	29780-29787	NN	denotes	Slc26a4
T7111	29787-29788	SYM	denotes	-
T7112	29788-29789	HYPH	denotes	/
T7113	29789-29790	SYM	denotes	-
T7110	29791-29795	NNS	denotes	mice
T7101	29796-29798	VBZ	denotes	is
T7114	29799-29809	JJ	denotes	consistent
T7115	29810-29814	IN	denotes	with
T7116	29815-29818	DT	denotes	the
T7117	29819-29826	NN	denotes	finding
T7118	29827-29829	IN	denotes	of
T7119	29830-29835	NN	denotes	KCNQ1
T7120	29836-29840	NN	denotes	mRNA
T7121	29841-29851	NN	denotes	expression
T7122	29852-29853	-LRB-	denotes	(
T7123	29853-29857	NN	denotes	Fig.
T7124	29858-29859	CD	denotes	3
T7125	29859-29860	-RRB-	denotes	)
T7126	29860-29861	.	denotes	.
T7127	29861-30072	sentence	denotes	These observations make it unlikely that the primary cause of the loss of the endocochlear potential is a compromise of K+ secretion by strial marginal cells or a compromise of gap junction mediated K+ cycling.
T7128	29862-29867	DT	denotes	These
T7129	29868-29880	NNS	denotes	observations
T7130	29881-29885	VBP	denotes	make
T7131	29886-29888	PRP	denotes	it
T7132	29889-29897	JJ	denotes	unlikely
T7133	29898-29902	IN	denotes	that
T7135	29903-29906	DT	denotes	the
T7137	29907-29914	JJ	denotes	primary
T7136	29915-29920	NN	denotes	cause
T7138	29921-29923	IN	denotes	of
T7139	29924-29927	DT	denotes	the
T7140	29928-29932	NN	denotes	loss
T7141	29933-29935	IN	denotes	of
T7142	29936-29939	DT	denotes	the
T7144	29940-29952	JJ	denotes	endocochlear
T7143	29953-29962	NN	denotes	potential
T7134	29963-29965	VBZ	denotes	is
T7145	29966-29967	DT	denotes	a
T7146	29968-29978	NN	denotes	compromise
T7147	29979-29981	IN	denotes	of
T7148	29982-29983	NN	denotes	K
T7150	29983-29984	SYM	denotes	+
T7149	29985-29994	NN	denotes	secretion
T7151	29995-29997	IN	denotes	by
T7152	29998-30004	JJ	denotes	strial
T7153	30005-30013	JJ	denotes	marginal
T7154	30014-30019	NNS	denotes	cells
T7155	30020-30022	CC	denotes	or
T7156	30023-30024	DT	denotes	a
T7157	30025-30035	NN	denotes	compromise
T7158	30036-30038	IN	denotes	of
T7159	30039-30042	NN	denotes	gap
T7160	30043-30051	NN	denotes	junction
T7161	30052-30060	VBN	denotes	mediated
T7163	30061-30062	NN	denotes	K
T7164	30062-30063	SYM	denotes	+
T7162	30064-30071	NN	denotes	cycling
T7165	30071-30072	.	denotes	.
T9850	30083-30090	NN	denotes	Protein
T9851	30091-30103	NN	denotes	localization
T9852	30104-30106	IN	denotes	of
T9853	30107-30112	NN	denotes	KCNQ1
T9854	30112-30114	,	denotes	,
T9855	30114-30119	NN	denotes	KCNE1
T9856	30119-30121	,	denotes	,
T9857	30121-30128	NN	denotes	SLC12A2
T9858	30129-30132	CC	denotes	and
T9859	30133-30137	NN	denotes	GJB2
T9860	30138-30140	IN	denotes	in
T9861	30141-30144	DT	denotes	the
T9863	30145-30153	JJ	denotes	cochlear
T9864	30154-30161	JJ	denotes	lateral
T9862	30162-30166	NN	denotes	wall
T9865	30167-30169	IN	denotes	of
T9866	30170-30177	NN	denotes	Slc26a4
T9868	30177-30178	SYM	denotes	+
T9869	30178-30179	HYPH	denotes	/
T9870	30179-30180	SYM	denotes	+
T9871	30181-30184	CC	denotes	and
T9872	30185-30192	NN	denotes	Slc26a4
T9873	30192-30193	SYM	denotes	-
T9874	30193-30194	HYPH	denotes	/
T9875	30194-30195	SYM	denotes	-
T9867	30196-30200	NNS	denotes	mice
T9876	30200-30201	.	denotes	.
T9877	30201-30219	sentence	denotes	a-f: bars: 10 μm.
T9878	30202-30203	LS	denotes	a
T9880	30203-30204	SYM	denotes	-
T9881	30204-30205	LS	denotes	f
T9882	30205-30207	:	denotes	:
T9883	30207-30211	NNS	denotes	bars
T9884	30211-30213	:	denotes	:
T9885	30213-30215	CD	denotes	10
T9879	30216-30218	NNS	denotes	μm
T9886	30218-30219	.	denotes	.
T9887	30219-30307	sentence	denotes	SMC, strial marginal cells; SV, stria vascularis; BC, basal cells; SL, spiral ligament.
T9888	30220-30223	NN	denotes	SMC
T9889	30223-30225	,	denotes	,
T9890	30225-30231	JJ	denotes	strial
T9892	30232-30240	JJ	denotes	marginal
T9891	30241-30246	NNS	denotes	cells
T9893	30246-30247	:	denotes	;
T9894	30248-30250	NN	denotes	SV
T9895	30250-30252	,	denotes	,
T9896	30252-30257	NN	denotes	stria
T9897	30258-30268	NN	denotes	vascularis
T9898	30268-30269	:	denotes	;
T9899	30270-30272	NN	denotes	BC
T9900	30272-30274	,	denotes	,
T9901	30274-30279	JJ	denotes	basal
T9902	30280-30285	NNS	denotes	cells
T9903	30285-30286	:	denotes	;
T9904	30287-30289	NN	denotes	SL
T9905	30289-30291	,	denotes	,
T9906	30291-30297	JJ	denotes	spiral
T9907	30298-30306	NN	denotes	ligament
T9908	30306-30307	.	denotes	.
T7393	30321-30323	PRP	denotes	We
T7394	30324-30333	VBD	denotes	described
T7395	30334-30343	NNS	denotes	locations
T7396	30344-30346	IN	denotes	of
T7397	30347-30354	NN	denotes	pendrin
T7398	30355-30365	NN	denotes	expression
T7399	30366-30368	IN	denotes	in
T7400	30369-30372	DT	denotes	the
T7401	30373-30380	NN	denotes	cochlea
T7402	30381-30384	CC	denotes	and
T7403	30385-30395	JJ	denotes	vestibular
T7404	30396-30405	NN	denotes	labyrinth
T7405	30406-30409	CC	denotes	and
T7406	30410-30418	VBD	denotes	detected
T7407	30419-30425	JJ	denotes	normal
T7409	30426-30439	JJ	denotes	endolymphatic
T7410	30440-30441	NN	denotes	K
T7411	30441-30442	SYM	denotes	+
T7408	30443-30457	NNS	denotes	concentrations
T7412	30458-30460	IN	denotes	in
T7413	30461-30466	NN	denotes	spite
T7414	30467-30469	IN	denotes	of
T7415	30470-30472	DT	denotes	an
T7416	30473-30484	NN	denotes	enlargement
T7417	30485-30487	IN	denotes	of
T7418	30488-30492	DT	denotes	this
T7420	30493-30498	NN	denotes	fluid
T7419	30499-30510	NN	denotes	compartment
T7421	30510-30511	.	denotes	.
T7422	30511-30617	sentence	denotes	We found that Slc26a4-/- mice lack the endocochlear potential because they do not express KCNJ10 protein.
T7423	30512-30514	PRP	denotes	We
T7424	30515-30520	VBD	denotes	found
T7425	30521-30525	IN	denotes	that
T7427	30526-30533	NN	denotes	Slc26a4
T7429	30533-30534	SYM	denotes	-
T7430	30534-30535	HYPH	denotes	/
T7431	30535-30536	SYM	denotes	-
T7428	30537-30541	NNS	denotes	mice
T7426	30542-30546	VBP	denotes	lack
T7432	30547-30550	DT	denotes	the
T7434	30551-30563	JJ	denotes	endocochlear
T7433	30564-30573	NN	denotes	potential
T7435	30574-30581	IN	denotes	because
T7437	30582-30586	PRP	denotes	they
T7438	30587-30589	VBP	denotes	do
T7439	30590-30593	RB	denotes	not
T7436	30594-30601	VB	denotes	express
T7440	30602-30608	NN	denotes	KCNJ10
T7441	30609-30616	NN	denotes	protein
T7442	30616-30617	.	denotes	.
T7443	30617-30732	sentence	denotes	Intermediate cells protect stria vascularis by converting CO2 to HCO3 - and by detoxifying free radicals (Fig. 9).
T7444	30618-30630	JJ	denotes	Intermediate
T7445	30631-30636	NNS	denotes	cells
T7446	30637-30644	VBP	denotes	protect
T7447	30645-30650	NN	denotes	stria
T7448	30651-30661	NN	denotes	vascularis
T7449	30662-30664	IN	denotes	by
T7450	30665-30675	VBG	denotes	converting
T7451	30676-30679	NN	denotes	CO2
T7452	30680-30682	IN	denotes	to
T7453	30683-30687	NN	denotes	HCO3
T7454	30688-30689	SYM	denotes	-
T7455	30690-30693	CC	denotes	and
T7456	30694-30696	IN	denotes	by
T7457	30697-30708	VBG	denotes	detoxifying
T7458	30709-30713	JJ	denotes	free
T7459	30714-30722	NNS	denotes	radicals
T7460	30723-30724	-LRB-	denotes	(
T7461	30724-30728	NN	denotes	Fig.
T7462	30729-30730	CD	denotes	9
T7463	30730-30731	-RRB-	denotes	)
T7464	30731-30732	.	denotes	.
T7465	30732-30863	sentence	denotes	CO2 and free radicals are generated by the large numbers of mitochondria in the metabolically highly active strial marginal cells.
T7466	30733-30736	NN	denotes	CO2
T7468	30737-30740	CC	denotes	and
T7469	30741-30745	JJ	denotes	free
T7470	30746-30754	NNS	denotes	radicals
T7471	30755-30758	VBP	denotes	are
T7467	30759-30768	VBN	denotes	generated
T7472	30769-30771	IN	denotes	by
T7473	30772-30775	DT	denotes	the
T7475	30776-30781	JJ	denotes	large
T7474	30782-30789	NNS	denotes	numbers
T7476	30790-30792	IN	denotes	of
T7477	30793-30805	NNS	denotes	mitochondria
T7478	30806-30808	IN	denotes	in
T7479	30809-30812	DT	denotes	the
T7481	30813-30826	RB	denotes	metabolically
T7483	30827-30833	RB	denotes	highly
T7482	30834-30840	JJ	denotes	active
T7484	30841-30847	JJ	denotes	strial
T7485	30848-30856	JJ	denotes	marginal
T7480	30857-30862	NNS	denotes	cells
T7486	30862-30863	.	denotes	.
T7487	30863-30981	sentence	denotes	Intermediate cells employ carbonic anhydrase to convert CO2 to HCO3 - [18,19] and catalase to detoxify free radicals.
T7488	30864-30876	JJ	denotes	Intermediate
T7489	30877-30882	NNS	denotes	cells
T7490	30883-30889	VBP	denotes	employ
T7491	30890-30898	JJ	denotes	carbonic
T7492	30899-30908	NN	denotes	anhydrase
T7493	30909-30911	TO	denotes	to
T7494	30912-30919	VB	denotes	convert
T7495	30920-30923	NN	denotes	CO2
T7496	30924-30926	IN	denotes	to
T7497	30927-30931	NN	denotes	HCO3
T7498	30932-30933	SYM	denotes	-
T7499	30934-30935	-LRB-	denotes	[
T7501	30935-30937	CD	denotes	18
T7502	30937-30938	,	denotes	,
T7500	30938-30940	CD	denotes	19
T7503	30940-30941	-RRB-	denotes	]
T7504	30942-30945	CC	denotes	and
T7505	30946-30954	NN	denotes	catalase
T7506	30955-30957	TO	denotes	to
T7507	30958-30966	VB	denotes	detoxify
T7508	30967-30971	JJ	denotes	free
T7509	30972-30980	NNS	denotes	radicals
T7510	30980-30981	.	denotes	.
T7511	30981-31098	sentence	denotes	To protect themselves from free radical damage, intermediate cells generate glutathione and melanin pigment [34-37].
T7512	30982-30984	TO	denotes	To
T7513	30985-30992	VB	denotes	protect
T7515	30993-31003	PRP	denotes	themselves
T7516	31004-31008	IN	denotes	from
T7517	31009-31013	JJ	denotes	free
T7519	31014-31021	NN	denotes	radical
T7518	31022-31028	NN	denotes	damage
T7520	31028-31030	,	denotes	,
T7521	31030-31042	JJ	denotes	intermediate
T7522	31043-31048	NNS	denotes	cells
T7514	31049-31057	VBP	denotes	generate
T7523	31058-31069	NN	denotes	glutathione
T7524	31070-31073	CC	denotes	and
T7525	31074-31081	NN	denotes	melanin
T7526	31082-31089	NN	denotes	pigment
T7527	31090-31091	-LRB-	denotes	[
T7528	31091-31093	CD	denotes	34
T7529	31093-31094	SYM	denotes	-
T7530	31094-31096	CD	denotes	37
T7531	31096-31097	-RRB-	denotes	]
T7532	31097-31098	.	denotes	.
T7533	31098-31265	sentence	denotes	It is conceivable that loss of pendrin, which may secrete HCO3 - into endolymph, results in an accumulation of HCO3 - and an alkalinization of the intrastrial spaces.
T7534	31099-31101	PRP	denotes	It
T7535	31102-31104	VBZ	denotes	is
T7536	31105-31116	JJ	denotes	conceivable
T7537	31117-31121	IN	denotes	that
T7539	31122-31126	NN	denotes	loss
T7540	31127-31129	IN	denotes	of
T7541	31130-31137	NN	denotes	pendrin
T7542	31137-31139	,	denotes	,
T7543	31139-31144	WDT	denotes	which
T7545	31145-31148	MD	denotes	may
T7544	31149-31156	VB	denotes	secrete
T7546	31157-31161	NN	denotes	HCO3
T7547	31162-31163	SYM	denotes	-
T7548	31164-31168	IN	denotes	into
T7549	31169-31178	NN	denotes	endolymph
T7550	31178-31180	,	denotes	,
T7538	31180-31187	VBZ	denotes	results
T7551	31188-31190	IN	denotes	in
T7552	31191-31193	DT	denotes	an
T7553	31194-31206	NN	denotes	accumulation
T7554	31207-31209	IN	denotes	of
T7555	31210-31214	NN	denotes	HCO3
T7556	31215-31216	SYM	denotes	-
T7557	31217-31220	CC	denotes	and
T7558	31221-31223	DT	denotes	an
T7559	31224-31238	NN	denotes	alkalinization
T7560	31239-31241	IN	denotes	of
T7561	31242-31245	DT	denotes	the
T7563	31246-31257	JJ	denotes	intrastrial
T7562	31258-31264	NNS	denotes	spaces
T7564	31264-31265	.	denotes	.
T7565	31265-31441	sentence	denotes	This extracellular alkalinization may enhance free radical stress, since it may inhibit the uptake of cysteine and thereby limit production of the protective glutathione [38].
T7566	31266-31270	DT	denotes	This
T7568	31271-31284	JJ	denotes	extracellular
T7567	31285-31299	NN	denotes	alkalinization
T7570	31300-31303	MD	denotes	may
T7569	31304-31311	VB	denotes	enhance
T7571	31312-31316	JJ	denotes	free
T7572	31317-31324	NN	denotes	radical
T7573	31325-31331	NN	denotes	stress
T7574	31331-31333	,	denotes	,
T7575	31333-31338	IN	denotes	since
T7577	31339-31341	PRP	denotes	it
T7578	31342-31345	MD	denotes	may
T7576	31346-31353	VB	denotes	inhibit
T7579	31354-31357	DT	denotes	the
T7580	31358-31364	NN	denotes	uptake
T7581	31365-31367	IN	denotes	of
T7582	31368-31376	NN	denotes	cysteine
T7583	31377-31380	CC	denotes	and
T7584	31381-31388	RB	denotes	thereby
T7585	31389-31394	VB	denotes	limit
T7586	31395-31405	NN	denotes	production
T7587	31406-31408	IN	denotes	of
T7588	31409-31412	DT	denotes	the
T7590	31413-31423	JJ	denotes	protective
T7589	31424-31435	NN	denotes	glutathione
T7591	31436-31437	-LRB-	denotes	[
T7592	31437-31439	CD	denotes	38
T7593	31439-31440	-RRB-	denotes	]
T7594	31440-31441	.	denotes	.
T7595	31441-31567	sentence	denotes	Support for the hypothesis of enhanced free radical stress comes from the observed hyperpigmentation in mice lacking pendrin.
T7596	31442-31449	NN	denotes	Support
T7598	31450-31453	IN	denotes	for
T7599	31454-31457	DT	denotes	the
T7600	31458-31468	NN	denotes	hypothesis
T7601	31469-31471	IN	denotes	of
T7602	31472-31480	VBN	denotes	enhanced
T7604	31481-31485	JJ	denotes	free
T7605	31486-31493	NN	denotes	radical
T7603	31494-31500	NN	denotes	stress
T7597	31501-31506	VBZ	denotes	comes
T7606	31507-31511	IN	denotes	from
T7607	31512-31515	DT	denotes	the
T7609	31516-31524	VBN	denotes	observed
T7608	31525-31542	NN	denotes	hyperpigmentation
T7610	31543-31545	IN	denotes	in
T7611	31546-31550	NNS	denotes	mice
T7612	31551-31558	VBG	denotes	lacking
T7613	31559-31566	NN	denotes	pendrin
T7614	31566-31567	.	denotes	.
T7615	31567-31711	sentence	denotes	Strial hyperpigmentation has also been observed in other conditions that are associated with free radical stress, such as acoustic trauma [37].
T7616	31568-31574	JJ	denotes	Strial
T7617	31575-31592	NN	denotes	hyperpigmentation
T7619	31593-31596	VBZ	denotes	has
T7620	31597-31601	RB	denotes	also
T7621	31602-31606	VBN	denotes	been
T7618	31607-31615	VBN	denotes	observed
T7622	31616-31618	IN	denotes	in
T7623	31619-31624	JJ	denotes	other
T7624	31625-31635	NNS	denotes	conditions
T7625	31636-31640	WDT	denotes	that
T7627	31641-31644	VBP	denotes	are
T7626	31645-31655	VBN	denotes	associated
T7628	31656-31660	IN	denotes	with
T7629	31661-31665	JJ	denotes	free
T7630	31666-31673	NN	denotes	radical
T7631	31674-31680	NN	denotes	stress
T7632	31680-31682	,	denotes	,
T7633	31682-31686	JJ	denotes	such
T7634	31687-31689	IN	denotes	as
T7635	31690-31698	JJ	denotes	acoustic
T7636	31699-31705	NN	denotes	trauma
T7637	31706-31707	-LRB-	denotes	[
T7638	31707-31709	CD	denotes	37
T7639	31709-31710	-RRB-	denotes	]
T7640	31710-31711	.	denotes	.
T7641	31711-31863	sentence	denotes	Alterations in the cytosolic pH in conjunction with free radical stress may lead to the loss of KCNJ10 protein expression in strial intermediate cells.
T7642	31712-31723	NNS	denotes	Alterations
T7644	31724-31726	IN	denotes	in
T7645	31727-31730	DT	denotes	the
T7647	31731-31740	JJ	denotes	cytosolic
T7646	31741-31743	NN	denotes	pH
T7648	31744-31746	IN	denotes	in
T7649	31747-31758	NN	denotes	conjunction
T7650	31759-31763	IN	denotes	with
T7651	31764-31768	JJ	denotes	free
T7652	31769-31776	NN	denotes	radical
T7653	31777-31783	NN	denotes	stress
T7654	31784-31787	MD	denotes	may
T7643	31788-31792	VB	denotes	lead
T7655	31793-31795	IN	denotes	to
T7656	31796-31799	DT	denotes	the
T7657	31800-31804	NN	denotes	loss
T7658	31805-31807	IN	denotes	of
T7659	31808-31814	NN	denotes	KCNJ10
T7661	31815-31822	NN	denotes	protein
T7660	31823-31833	NN	denotes	expression
T7662	31834-31836	IN	denotes	in
T7663	31837-31843	JJ	denotes	strial
T7665	31844-31856	JJ	denotes	intermediate
T7664	31857-31862	NNS	denotes	cells
T7666	31862-31863	.	denotes	.
T7667	31863-32030	sentence	denotes	Function and expression of other K+ channels has been shown to be controlled by the cytosolic pH and free radicals, which encode the metabolic state of the cell [39].
T7668	31864-31872	NN	denotes	Function
T7670	31873-31876	CC	denotes	and
T7671	31877-31887	NN	denotes	expression
T7672	31888-31890	IN	denotes	of
T7673	31891-31896	JJ	denotes	other
T7675	31897-31898	NN	denotes	K
T7676	31898-31899	SYM	denotes	+
T7674	31900-31908	NNS	denotes	channels
T7677	31909-31912	VBZ	denotes	has
T7678	31913-31917	VBN	denotes	been
T7669	31918-31923	VBN	denotes	shown
T7679	31924-31926	TO	denotes	to
T7681	31927-31929	VB	denotes	be
T7680	31930-31940	VBN	denotes	controlled
T7682	31941-31943	IN	denotes	by
T7683	31944-31947	DT	denotes	the
T7685	31948-31957	JJ	denotes	cytosolic
T7684	31958-31960	NN	denotes	pH
T7686	31961-31964	CC	denotes	and
T7687	31965-31969	JJ	denotes	free
T7688	31970-31978	NNS	denotes	radicals
T7689	31978-31980	,	denotes	,
T7690	31980-31985	WDT	denotes	which
T7691	31986-31992	VBP	denotes	encode
T7692	31993-31996	DT	denotes	the
T7694	31997-32006	JJ	denotes	metabolic
T7693	32007-32012	NN	denotes	state
T7695	32013-32015	IN	denotes	of
T7696	32016-32019	DT	denotes	the
T7697	32020-32024	NN	denotes	cell
T7698	32025-32026	-LRB-	denotes	[
T7699	32026-32028	CD	denotes	39
T7700	32028-32029	-RRB-	denotes	]
T7701	32029-32030	.	denotes	.
T7702	32030-32272	sentence	denotes	Suppression of the KCNJ10 K+ channel in strial intermediate cells, which is essential for the generation of the endocochlear potential, is probably the direct cause of deafness in Slc26a4-/- mice and patients suffering from Pendred syndrome.
T7703	32031-32042	NN	denotes	Suppression
T7705	32043-32045	IN	denotes	of
T7706	32046-32049	DT	denotes	the
T7708	32050-32056	NN	denotes	KCNJ10
T7709	32057-32058	NN	denotes	K
T7710	32058-32059	SYM	denotes	+
T7707	32060-32067	NN	denotes	channel
T7711	32068-32070	IN	denotes	in
T7712	32071-32077	JJ	denotes	strial
T7714	32078-32090	JJ	denotes	intermediate
T7713	32091-32096	NNS	denotes	cells
T7715	32096-32098	,	denotes	,
T7716	32098-32103	WDT	denotes	which
T7717	32104-32106	VBZ	denotes	is
T7718	32107-32116	JJ	denotes	essential
T7719	32117-32120	IN	denotes	for
T7720	32121-32124	DT	denotes	the
T7721	32125-32135	NN	denotes	generation
T7722	32136-32138	IN	denotes	of
T7723	32139-32142	DT	denotes	the
T7725	32143-32155	JJ	denotes	endocochlear
T7724	32156-32165	NN	denotes	potential
T7726	32165-32167	,	denotes	,
T7704	32167-32169	VBZ	denotes	is
T7727	32170-32178	RB	denotes	probably
T7728	32179-32182	DT	denotes	the
T7730	32183-32189	JJ	denotes	direct
T7729	32190-32195	NN	denotes	cause
T7731	32196-32198	IN	denotes	of
T7732	32199-32207	NN	denotes	deafness
T7733	32208-32210	IN	denotes	in
T7734	32211-32218	NN	denotes	Slc26a4
T7736	32218-32219	SYM	denotes	-
T7737	32219-32220	HYPH	denotes	/
T7738	32220-32221	SYM	denotes	-
T7735	32222-32226	NNS	denotes	mice
T7739	32227-32230	CC	denotes	and
T7740	32231-32239	NNS	denotes	patients
T7741	32240-32249	VBG	denotes	suffering
T7742	32250-32254	IN	denotes	from
T7743	32255-32262	NNP	denotes	Pendred
T7744	32263-32271	NN	denotes	syndrome
T7745	32271-32272	.	denotes	.
T9958	32283-32288	NN	denotes	Model
T9959	32289-32292	IN	denotes	for
T9960	32293-32296	DT	denotes	the
T9961	32297-32301	NN	denotes	loss
T9962	32302-32304	IN	denotes	of
T9963	32305-32311	NN	denotes	KCNJ10
T9964	32312-32314	IN	denotes	in
T9965	32315-32318	DT	denotes	the
T9966	32319-32326	NN	denotes	absence
T9967	32327-32329	IN	denotes	of
T9968	32330-32337	NN	denotes	pendrin
T9969	32338-32348	NN	denotes	expression
T9970	32349-32351	IN	denotes	in
T9971	32352-32357	NN	denotes	stria
T9972	32358-32368	NN	denotes	vascularis
T9973	32368-32369	.	denotes	.
T9974	32369-32488	sentence	denotes	Cys, cysteine, Glu, glutamate, Gly, glycine, CA, carbonic anhydrase, GST, glutathione-S-transferase, GSH, glutathione.
T9975	32370-32373	NN	denotes	Cys
T9976	32373-32375	,	denotes	,
T9977	32375-32383	NN	denotes	cysteine
T9978	32383-32385	,	denotes	,
T9979	32385-32388	NN	denotes	Glu
T9980	32388-32390	,	denotes	,
T9981	32390-32399	NN	denotes	glutamate
T9982	32399-32401	,	denotes	,
T9983	32401-32404	NN	denotes	Gly
T9984	32404-32406	,	denotes	,
T9985	32406-32413	NN	denotes	glycine
T9986	32413-32415	,	denotes	,
T9987	32415-32417	NN	denotes	CA
T9988	32417-32419	,	denotes	,
T9989	32419-32427	JJ	denotes	carbonic
T9990	32428-32437	NN	denotes	anhydrase
T9991	32437-32439	,	denotes	,
T9992	32439-32442	NN	denotes	GST
T9993	32442-32444	,	denotes	,
T9994	32444-32455	NN	denotes	glutathione
T9996	32455-32456	HYPH	denotes	-
T9997	32456-32457	NN	denotes	S
T9998	32457-32458	HYPH	denotes	-
T9995	32458-32469	NN	denotes	transferase
T9999	32469-32471	,	denotes	,
T10000	32471-32474	NN	denotes	GSH
T10001	32474-32476	,	denotes	,
T10002	32476-32487	NN	denotes	glutathione
T10003	32487-32488	.	denotes	.
T7746	33281-33284	DT	denotes	The
T7748	33285-33300	JJ	denotes	pre-publication
T7747	33301-33308	NN	denotes	history
T7750	33309-33312	IN	denotes	for
T7751	33313-33317	DT	denotes	this
T7752	33318-33323	NN	denotes	paper
T7753	33324-33327	MD	denotes	can
T7754	33328-33330	VB	denotes	be
T7749	33331-33339	VBN	denotes	accessed
T7755	33340-33344	RB	denotes	here
T7756	33344-33345	:	denotes	:
R10	T239	T237	pobj	expression,of
R100	T343	T344	det	The,potential
R1000	T1739	T1738	prep	with,infiltrated
R1001	T1740	T1741	compound	polyethylene,glycol
R1002	T1741	T1739	pobj	glycol,with
R1003	T1742	T1723	punct	.,fixed
R1004	T1744	T1745	amod	Mid-modiolar,cryosections
R1005	T1745	T1746	nsubjpass	cryosections,blocked
R1006	T1747	T1748	punct	(,CM3050S
R1007	T1748	T1745	parataxis	CM3050S,cryosections
R1008	T1749	T1750	nummod	12,μm
R1009	T1750	T1748	nmod	μm,CM3050S
R101	T344	T346	nsubjpass	potential,measured
R1010	T1751	T1748	punct	", ",CM3050S
R1011	T1752	T1748	punct	", ",CM3050S
R1012	T1753	T1748	npadvmod	Leica,CM3050S
R1013	T1754	T1748	punct	", ",CM3050S
R1014	T1755	T1748	npadvmod	Nussloch,CM3050S
R1015	T1756	T1748	punct	", ",CM3050S
R1016	T1757	T1748	npadvmod	Germany,CM3050S
R1017	T1758	T1748	punct	),CM3050S
R1018	T1759	T1746	auxpass	were,blocked
R1019	T1760	T1746	prep	in,blocked
R102	T345	T344	amod	endocochlear,potential
R1020	T1761	T1760	pobj	PBS,in
R1021	T1762	T1746	prep	with,blocked
R1022	T1763	T1764	nummod	0.2,%
R1023	T1764	T1765	compound	%,X
R1024	T1765	T1762	pobj	X,with
R1025	T1766	T1765	compound	Triton,X
R1026	T1767	T1765	punct	-,X
R1027	T1768	T1769	punct	(,TX
R1028	T1769	T1765	parataxis	TX,X
R1029	T1770	T1769	compound	PBS,TX
R103	T347	T344	cc	and,potential
R1030	T1771	T1769	punct	-,TX
R1031	T1772	T1769	punct	),TX
R1032	T1773	T1765	cc	and,X
R1033	T1774	T1775	nummod	10,%
R1034	T1775	T1776	nmod	%,albumin
R1035	T1776	T1765	conj	albumin,X
R1036	T1777	T1776	amod	bovine,albumin
R1037	T1778	T1776	compound	serum,albumin
R1038	T1779	T1746	punct	.,blocked
R1039	T1781	T1782	nsubjpass	Slides,incubated
R104	T348	T349	det	the,concentration
R1040	T1783	T1782	auxpass	were,incubated
R1041	T1784	T1782	advmod	overnight,incubated
R1042	T1785	T1782	prep	at,incubated
R1043	T1786	T1787	nummod	4,°C
R1044	T1787	T1785	pobj	°C,at
R1045	T1788	T1782	prep	with,incubated
R1046	T1789	T1790	amod	primary,antibodies
R1047	T1790	T1788	pobj	antibodies,with
R1048	T1791	T1782	prep	in,incubated
R1049	T1792	T1793	compound	PBS,TX
R105	T349	T344	conj	concentration,potential
R1050	T1793	T1791	pobj	TX,in
R1051	T1794	T1793	punct	-,TX
R1052	T1795	T1782	prep	with,incubated
R1053	T1796	T1797	quantmod	1,3
R1054	T1797	T1799	nummod	3,%
R1055	T1798	T1797	punct	–,3
R1056	T1799	T1800	compound	%,BSA
R1057	T1800	T1795	pobj	BSA,with
R1058	T1801	T1802	punct	[,h766
R1059	T1802	T1782	parataxis	h766,incubated
R106	T350	T349	amod	endolymphatic,concentration
R1060	T1803	T1804	compound	rabbit,anti-pendrin
R1061	T1804	T1802	dep	anti-pendrin,h766
R1062	T1805	T1802	punct	", ",h766
R1063	T1806	T1802	dep	1,h766
R1064	T1807	T1808	punct	:,500
R1065	T1808	T1806	prep	500,1
R1066	T1809	T1802	punct	(,h766
R1067	T1810	T1811	punct	–,780
R1068	T1811	T1802	prep	780,h766
R1069	T1812	T1802	punct	),h766
R107	T351	T349	nmod	K,concentration
R1070	T1813	T1802	punct	;,h766
R1071	T1814	T1815	compound	rat,anti-ZO
R1072	T1815	T1816	dep	anti-ZO,Chemicon
R1073	T1816	T1802	conj	Chemicon,h766
R1074	T1817	T1815	punct	-,anti-ZO
R1075	T1818	T1815	nummod	1,anti-ZO
R1076	T1819	T1816	punct	", ",Chemicon
R1077	T1820	T1816	dep	1,Chemicon
R1078	T1821	T1822	punct	:,100
R1079	T1822	T1820	prep	100,1
R108	T352	T351	punct	+,K
R1080	T1823	T1816	punct	(,Chemicon
R1081	T1824	T1816	punct	", ",Chemicon
R1082	T1825	T1816	npadvmod	Temecula,Chemicon
R1083	T1826	T1816	punct	", ",Chemicon
R1084	T1827	T1816	npadvmod	CA,Chemicon
R1085	T1828	T1816	punct	),Chemicon
R1086	T1829	T1816	punct	;,Chemicon
R1087	T1830	T1831	compound	goat,anti-KCNQ1
R1088	T1831	T1832	dep	anti-KCNQ1,C20
R1089	T1832	T1816	conj	C20,Chemicon
R109	T353	T346	auxpass	were,measured
R1090	T1833	T1832	punct	", ",C20
R1091	T1834	T1832	dep	1,C20
R1092	T1835	T1836	punct	:,200
R1093	T1836	T1834	prep	200,1
R1094	T1837	T1832	punct	(,C20
R1095	T1838	T1832	punct	", ",C20
R1096	T1839	T1840	compound	Santa,Cruz
R1097	T1840	T1841	compound	Cruz,Biotechnology
R1098	T1841	T1832	npadvmod	Biotechnology,C20
R1099	T1842	T1832	punct	", ",C20
R11	T240	T239	compound	protein,expression
R110	T354	T346	prep	with,measured
R1100	T1843	T1844	compound	Santa,Cruz
R1101	T1844	T1832	npadvmod	Cruz,C20
R1102	T1845	T1832	punct	", ",C20
R1103	T1846	T1832	npadvmod	CA,C20
R1104	T1847	T1832	punct	),C20
R1105	T1848	T1832	punct	", ",C20
R1106	T1849	T1850	compound	rabbit,anti-KCNE1
R1107	T1850	T1851	dep	anti-KCNE1,Alomone
R1108	T1851	T1832	conj	Alomone,C20
R1109	T1852	T1851	punct	", ",Alomone
R111	T355	T356	amod	double,barreled
R1110	T1853	T1851	dep	1,Alomone
R1111	T1854	T1855	punct	:,200
R1112	T1855	T1853	prep	200,1
R1113	T1856	T1851	punct	(,Alomone
R1114	T1857	T1851	punct	", ",Alomone
R1115	T1858	T1851	npadvmod	Jerusalem,Alomone
R1116	T1859	T1851	punct	", ",Alomone
R1117	T1860	T1851	npadvmod	Israel,Alomone
R1118	T1861	T1851	punct	),Alomone
R1119	T1862	T1851	punct	;,Alomone
R112	T356	T358	amod	barreled,microelectrodes
R1120	T1863	T1864	compound	rabbit,anti-KCNJ10
R1121	T1864	T1865	dep	anti-KCNJ10,Alomone
R1122	T1865	T1851	conj	Alomone,Alomone
R1123	T1866	T1865	punct	", ",Alomone
R1124	T1867	T1865	dep	1,Alomone
R1125	T1868	T1869	punct	:,300
R1126	T1869	T1867	prep	300,1
R1127	T1870	T1865	punct	(,Alomone
R1128	T1871	T1865	punct	),Alomone
R1129	T1872	T1865	punct	;,Alomone
R113	T357	T356	punct	-,barreled
R1130	T1873	T1874	compound	rabbit,anti-SLC12A2
R1131	T1874	T1875	dep	anti-SLC12A2,Chemicon
R1132	T1875	T1865	conj	Chemicon,Alomone
R1133	T1876	T1875	punct	", ",Chemicon
R1134	T1877	T1875	dep	1,Chemicon
R1135	T1878	T1879	punct	:,100
R1136	T1879	T1877	prep	100,1
R1137	T1880	T1875	punct	(,Chemicon
R1138	T1881	T1875	punct	),Chemicon
R1139	T1882	T1875	punct	;,Chemicon
R114	T358	T354	pobj	microelectrodes,with
R1140	T1883	T1875	cc	and,Chemicon
R1141	T1884	T1885	compound	rabbit,anti-connexin
R1142	T1885	T1886	dep	anti-connexin,Zymed
R1143	T1886	T1875	conj	Zymed,Chemicon
R1144	T1887	T1885	nummod	26,anti-connexin
R1145	T1888	T1886	punct	", ",Zymed
R1146	T1889	T1886	dep	1,Zymed
R1147	T1890	T1891	punct	:,100
R1148	T1891	T1889	prep	100,1
R1149	T1892	T1886	punct	(,Zymed
R115	T359	T346	punct	.,measured
R1150	T1893	T1886	punct	", ",Zymed
R1151	T1894	T1895	compound	San,Francisco
R1152	T1895	T1886	npadvmod	Francisco,Zymed
R1153	T1896	T1886	punct	", ",Zymed
R1154	T1897	T1886	npadvmod	CA,Zymed
R1155	T1898	T1886	punct	),Zymed
R1156	T1899	T1886	punct	],Zymed
R1157	T1900	T1782	punct	.,incubated
R1158	T1902	T1903	nsubjpass	Slides,washed
R1159	T1904	T1903	auxpass	were,washed
R116	T361	T362	nsubjpass	Currents,recorded
R1160	T1905	T1903	prep	in,washed
R1161	T1906	T1907	compound	PBS,TX
R1162	T1907	T1905	pobj	TX,in
R1163	T1908	T1907	punct	-,TX
R1164	T1909	T1903	cc	and,washed
R1165	T1910	T1903	conj	incubated,washed
R1166	T1911	T1910	prep	for,incubated
R1167	T1912	T1913	nummod	1,h
R1168	T1913	T1911	pobj	h,for
R1169	T1914	T1910	prep	at,incubated
R117	T363	T361	acl	generated,Currents
R1170	T1915	T1916	nummod	25,°C
R1171	T1916	T1914	pobj	°C,at
R1172	T1917	T1910	prep	with,incubated
R1173	T1918	T1919	amod	appropriate,antibodies
R1174	T1919	T1917	pobj	antibodies,with
R1175	T1920	T1919	amod	secondary,antibodies
R1176	T1921	T1919	prep	at,antibodies
R1177	T1922	T1923	det	a,dilution
R1178	T1923	T1921	pobj	dilution,at
R1179	T1924	T1923	nummod	1,dilution
R118	T364	T363	agent	by,generated
R1180	T1925	T1926	punct	:,1000
R1181	T1926	T1924	prep	1000,1
R1182	T1927	T1923	prep	in,dilution
R1183	T1928	T1929	compound	PBS,TX
R1184	T1929	T1927	pobj	TX,in
R1185	T1930	T1929	punct	-,TX
R1186	T1931	T1923	prep	with,dilution
R1187	T1932	T1933	quantmod	1,3
R1188	T1933	T1935	nummod	3,%
R1189	T1934	T1933	punct	–,3
R119	T365	T366	det	the,cells
R1190	T1935	T1936	compound	%,BSA
R1191	T1936	T1931	pobj	BSA,with
R1192	T1937	T1938	punct	[,Probes
R1193	T1938	T1910	parataxis	Probes,incubated
R1194	T1939	T1940	nmod	donkey,Alexa
R1195	T1940	T1938	dep	Alexa,Probes
R1196	T1941	T1939	amod	anti-rabbit,donkey
R1197	T1942	T1940	nummod	488,Alexa
R1198	T1943	T1940	punct	", ",Alexa
R1199	T1944	T1945	nmod	chicken,Alexa
R12	T241	T242	amod	endocochlear,potential
R120	T366	T364	pobj	cells,by
R1200	T1945	T1940	conj	Alexa,Alexa
R1201	T1946	T1944	amod	anti-rat,chicken
R1202	T1947	T1945	nummod	594,Alexa
R1203	T1948	T1945	punct	", ",Alexa
R1204	T1949	T1945	cc	and,Alexa
R1205	T1950	T1951	nmod	chicken,Alexa
R1206	T1951	T1945	conj	Alexa,Alexa
R1207	T1952	T1950	amod	anti-goat,chicken
R1208	T1953	T1951	nummod	594,Alexa
R1209	T1954	T1938	punct	(,Probes
R121	T367	T366	nmod	stria,cells
R1210	T1955	T1938	compound	Molecular,Probes
R1211	T1956	T1938	punct	", ",Probes
R1212	T1957	T1938	npadvmod	Eugene,Probes
R1213	T1958	T1938	punct	", ",Probes
R1214	T1959	T1938	npadvmod	OR,Probes
R1215	T1960	T1938	punct	),Probes
R1216	T1961	T1938	punct	],Probes
R1217	T1962	T1903	punct	.,washed
R1218	T1964	T1965	compound	Actin,filaments
R1219	T1965	T1966	nsubjpass	filaments,visualized
R122	T368	T366	amod	marginal,cells
R1220	T1967	T1966	auxpass	were,visualized
R1221	T1968	T1966	prep	by,visualized
R1222	T1969	T1968	pcomp	staining,by
R1223	T1970	T1969	prep	with,staining
R1224	T1971	T1972	npadvmod	Alexa,conjugated
R1225	T1972	T1974	amod	conjugated,phalloidin
R1226	T1973	T1971	nummod	488,Alexa
R1227	T1974	T1970	pobj	phalloidin,with
R1228	T1975	T1976	punct	(,Probes
R1229	T1976	T1974	parataxis	Probes,phalloidin
R123	T369	T362	auxpass	were,recorded
R1230	T1977	T1976	compound	Molecular,Probes
R1231	T1978	T1976	punct	),Probes
R1232	T1979	T1966	punct	.,visualized
R1233	T1981	T1982	prep	After,washed
R1234	T1983	T1981	pobj	incubation,After
R1235	T1984	T1982	punct	", ",washed
R1236	T1985	T1982	nsubjpass	slides,washed
R1237	T1986	T1982	auxpass	were,washed
R1238	T1987	T1982	prep	with,washed
R1239	T1988	T1989	compound	PBS,TX
R124	T370	T362	prep	with,recorded
R1240	T1989	T1987	pobj	TX,with
R1241	T1990	T1989	punct	-,TX
R1242	T1991	T1982	punct	", ",washed
R1243	T1992	T1982	conj	mounted,washed
R1244	T1993	T1992	prep	with,mounted
R1245	T1994	T1993	pobj	FluorSave,with
R1246	T1995	T1996	punct	(,Calbiochem
R1247	T1996	T1994	parataxis	Calbiochem,FluorSave
R1248	T1997	T1996	punct	", ",Calbiochem
R1249	T1998	T1999	compound	La,Jolla
R125	T371	T372	det	a,probe
R1250	T1999	T1996	npadvmod	Jolla,Calbiochem
R1251	T2000	T1996	punct	", ",Calbiochem
R1252	T2001	T1996	npadvmod	CA,Calbiochem
R1253	T2002	T1996	punct	),Calbiochem
R1254	T2003	T1992	punct	", ",mounted
R1255	T2004	T1992	cc	and,mounted
R1256	T2005	T1992	conj	viewed,mounted
R1257	T2006	T2005	prep	by,viewed
R1258	T2007	T2008	amod	confocal,microscopy
R1259	T2008	T2006	pobj	microscopy,by
R126	T372	T370	pobj	probe,with
R1260	T2009	T2010	punct	(,Pascal
R1261	T2010	T2008	parataxis	Pascal,microscopy
R1262	T2011	T2010	nmod	LSM,Pascal
R1263	T2012	T2010	nummod	5,Pascal
R1264	T2013	T2010	cc	or,Pascal
R1265	T2014	T2015	nmod	LSM,Meta
R1266	T2015	T2010	conj	Meta,Pascal
R1267	T2016	T2015	nummod	510,Meta
R1268	T2017	T2010	punct	", ",Pascal
R1269	T2018	T2019	compound	Carl,Zeiss
R127	T373	T372	amod	vibrating,probe
R1270	T2019	T2010	npadvmod	Zeiss,Pascal
R1271	T2020	T2010	punct	", ",Pascal
R1272	T2021	T2010	npadvmod	Jena,Pascal
R1273	T2022	T2021	cc	or,Jena
R1274	T2023	T2021	conj	Göttingen,Jena
R1275	T2024	T2010	punct	", ",Pascal
R1276	T2025	T2010	npadvmod	Germany,Pascal
R1277	T2026	T2010	punct	),Pascal
R1278	T2027	T1982	punct	.,washed
R1279	T2029	T2030	compound	Laser,scanning
R128	T374	T362	punct	.,recorded
R1280	T2030	T2031	compound	scanning,images
R1281	T2031	T2033	nsubjpass	images,collected
R1282	T2032	T2031	compound	brightfield,images
R1283	T2034	T2033	auxpass	were,collected
R1284	T2035	T2036	aux	to,document
R1285	T2036	T2033	advcl	document,collected
R1286	T2037	T2038	amod	structural,preservation
R1287	T2038	T2036	dobj	preservation,document
R1288	T2039	T2033	punct	", ",collected
R1289	T2040	T2033	prep	for,collected
R129	T376	T377	compound	Tissue,masses
R1290	T2041	T2042	amod	morphometric,analysis
R1291	T2042	T2040	pobj	analysis,for
R1292	T2043	T2040	cc	and,for
R1293	T2044	T2040	conj	for,for
R1294	T2045	T2044	pobj	analysis,for
R1295	T2046	T2045	prep	of,analysis
R1296	T2047	T2046	pobj	pigmentation,of
R1297	T2048	T2033	punct	.,collected
R1298	T2210	T2211	amod	Quantitative,PCR
R1299	T2212	T2211	compound	RT,PCR
R13	T242	T235	dobj	potential,abolishes
R130	T377	T378	nsubjpass	masses,evaluated
R1300	T2213	T2211	punct	-,PCR
R1301	T2215	T2216	nsubjpass	Animals,anesthetized
R1302	T2217	T2216	auxpass	were,anesthetized
R1303	T2218	T2216	advmod	deeply,anesthetized
R1304	T2219	T2216	prep	with,anesthetized
R1305	T2220	T2221	compound	sodium,pentobarbital
R1306	T2221	T2219	pobj	pentobarbital,with
R1307	T2222	T2223	punct	(,mg
R1308	T2223	T2221	parataxis	mg,pentobarbital
R1309	T2224	T2223	nummod	100,mg
R131	T379	T378	auxpass	were,evaluated
R1310	T2225	T2226	punct	/,kg
R1311	T2226	T2223	prep	kg,mg
R1312	T2227	T2223	advmod	i.p.,mg
R1313	T2228	T2223	punct	),mg
R1314	T2229	T2216	punct	.,anesthetized
R1315	T2231	T2232	nsubjpass	Kidneys,removed
R1316	T2233	T2231	cc	and,Kidneys
R1317	T2234	T2231	conj	brain,Kidneys
R1318	T2235	T2232	auxpass	were,removed
R1319	T2236	T2232	cc	and,removed
R132	T380	T378	prep	by,evaluated
R1320	T2237	T2232	conj	pulverized,removed
R1321	T2238	T2237	prep	in,pulverized
R1322	T2239	T2240	compound	liquid,N2
R1323	T2240	T2238	pobj	N2,in
R1324	T2241	T2232	punct	.,removed
R1325	T2243	T2244	amod	Temporal,bones
R1326	T2244	T2245	nsubjpass	bones,removed
R1327	T2246	T2245	auxpass	were,removed
R1328	T2247	T2245	cc	and,removed
R1329	T2248	T2249	nummod	two,preparations
R133	T381	T382	amod	morphometric,measurements
R1330	T2249	T2250	nsubjpass	preparations,obtained
R1331	T2250	T2245	conj	obtained,removed
R1332	T2251	T2249	prep	of,preparations
R1333	T2252	T2253	amod	inner,tissues
R1334	T2253	T2251	pobj	tissues,of
R1335	T2254	T2253	compound	ear,tissues
R1336	T2255	T2250	auxpass	were,obtained
R1337	T2256	T2250	prep	by,obtained
R1338	T2257	T2256	pobj	microdissection,by
R1339	T2258	T2250	punct	: ,obtained
R134	T382	T380	pobj	measurements,by
R1340	T2259	T2260	punct	(,1
R1341	T2260	T2261	meta	1,vascularis
R1342	T2261	T2250	dobj	vascularis,obtained
R1343	T2262	T2260	punct	),1
R1344	T2263	T2261	compound	stria,vascularis
R1345	T2264	T2261	prep	without,vascularis
R1346	T2265	T2266	amod	spiral,ligament
R1347	T2266	T2264	pobj	ligament,without
R1348	T2267	T2261	punct	", ",vascularis
R1349	T2268	T2261	cc	and,vascularis
R135	T383	T382	compound	distance,measurements
R1350	T2269	T2270	punct	(,2
R1351	T2270	T2271	meta	2,ganglia
R1352	T2271	T2261	conj	ganglia,vascularis
R1353	T2272	T2270	punct	),2
R1354	T2273	T2271	amod	spiral,ganglia
R1355	T2274	T2271	prep	including,ganglia
R1356	T2275	T2276	det	the,bone
R1357	T2276	T2274	pobj	bone,including
R1358	T2277	T2276	amod	surrounding,bone
R1359	T2278	T2276	cc	and,bone
R136	T384	T378	cc	and,evaluated
R1360	T2279	T2280	det	the,organ
R1361	T2280	T2276	conj	organ,bone
R1362	T2281	T2280	prep	of,organ
R1363	T2282	T2281	pobj	Corti,of
R1364	T2283	T2250	punct	.,obtained
R1365	T2285	T2286	det	The,medium
R1366	T2286	T2288	nsubjpass	medium,changed
R1367	T2287	T2286	compound	dissection,medium
R1368	T2289	T2288	auxpass	was,changed
R1369	T2290	T2288	advmod	twice,changed
R137	T385	T386	nsubjpass	pigmentation,quantified
R1370	T2291	T2288	prep	during,changed
R1371	T2292	T2293	det	the,microdissection
R1372	T2293	T2291	pobj	microdissection,during
R1373	T2294	T2288	cc	and,changed
R1374	T2295	T2296	amod	isolated,tissues
R1375	T2296	T2297	nsubjpass	tissues,washed
R1376	T2297	T2288	conj	washed,changed
R1377	T2298	T2297	auxpass	were,washed
R1378	T2299	T2300	nummod	three,times
R1379	T2300	T2297	npadvmod	times,washed
R138	T386	T378	conj	quantified,evaluated
R1380	T2301	T2302	aux	to,minimize
R1381	T2302	T2297	advcl	minimize,washed
R1382	T2303	T2302	dobj	cross-contamination,minimize
R1383	T2304	T2297	punct	.,washed
R1384	T2306	T2307	det	The,genotype
R1385	T2307	T2312	nsubjpass	genotype,verified
R1386	T2308	T2307	nmod	Slc26a4,genotype
R1387	T2309	T2308	punct	-,Slc26a4
R1388	T2310	T2308	punct	/,Slc26a4
R1389	T2311	T2308	punct	-,Slc26a4
R139	T387	T386	auxpass	was,quantified
R1390	T2313	T2312	auxpass	was,verified
R1391	T2314	T2312	prep	by,verified
R1392	T2315	T2316	det	the,observation
R1393	T2316	T2314	pobj	observation,by
R1394	T2317	T2316	prep	of,observation
R1395	T2318	T2319	amod	large,otoconia
R1396	T2319	T2317	pobj	otoconia,of
R1397	T2320	T2316	prep	in,observation
R1398	T2321	T2322	det	the,macula
R1399	T2322	T2320	pobj	macula,in
R14	T243	T235	cc	and,abolishes
R140	T388	T386	prep	by,quantified
R1400	T2323	T2322	amod	utricular,macula
R1401	T2324	T2325	punct	[,14
R1402	T2325	T2312	parataxis	14,verified
R1403	T2326	T2325	punct	],14
R1404	T2327	T2312	punct	.,verified
R1405	T2329	T2330	amod	Total,RNA
R1406	T2330	T2331	nsubjpass	RNA,isolated
R1407	T2332	T2331	auxpass	was,isolated
R1408	T2333	T2331	cc	and,isolated
R1409	T2334	T2335	amod	residual,contamination
R141	T389	T388	pobj	densitometry,by
R1410	T2335	T2337	nsubjpass	contamination,removed
R1411	T2336	T2335	compound	DNA,contamination
R1412	T2337	T2331	conj	removed,isolated
R1413	T2338	T2337	auxpass	was,removed
R1414	T2339	T2337	prep	by,removed
R1415	T2340	T2341	amod	enzymatic,digestion
R1416	T2341	T2339	pobj	digestion,by
R1417	T2342	T2343	punct	(,micro
R1418	T2343	T2337	parataxis	micro,removed
R1419	T2344	T2343	compound	RNeasy,micro
R142	T390	T386	punct	.,quantified
R1420	T2345	T2343	punct	", ",micro
R1421	T2346	T2343	npadvmod	Qiagen,micro
R1422	T2347	T2343	punct	", ",micro
R1423	T2348	T2343	npadvmod	Valencia,micro
R1424	T2349	T2343	punct	", ",micro
R1425	T2350	T2343	npadvmod	CA,micro
R1426	T2351	T2343	punct	),micro
R1427	T2352	T2337	punct	.,removed
R1428	T2354	T2355	nsubjpass	Quality,determined
R1429	T2356	T2354	cc	and,Quality
R143	T394	T395	nsubjpass	Pendrin,found
R1430	T2357	T2354	conj	quantity,Quality
R1431	T2358	T2354	prep	of,Quality
R1432	T2359	T2360	compound	18S,rRNA
R1433	T2360	T2358	pobj	rRNA,of
R1434	T2361	T2360	acl	obtained,rRNA
R1435	T2362	T2361	prep	from,obtained
R1436	T2363	T2362	pobj	kidneys,from
R1437	T2364	T2363	cc	and,kidneys
R1438	T2365	T2363	conj	brain,kidneys
R1439	T2366	T2355	auxpass	were,determined
R144	T396	T395	auxpass	was,found
R1440	T2367	T2368	punct	(,Assay
R1441	T2368	T2355	parataxis	Assay,determined
R1442	T2369	T2368	compound	Nano,Assay
R1443	T2370	T2368	punct	", ",Assay
R1444	T2371	T2372	nummod	2100,Bioanalyzer
R1445	T2372	T2368	appos	Bioanalyzer,Assay
R1446	T2373	T2368	punct	", ",Assay
R1447	T2374	T2368	npadvmod	Agilent,Assay
R1448	T2375	T2368	punct	", ",Assay
R1449	T2376	T2377	compound	Palo,Alto
R145	T397	T395	prep	in,found
R1450	T2377	T2368	npadvmod	Alto,Assay
R1451	T2378	T2368	punct	", ",Assay
R1452	T2379	T2368	npadvmod	CA,Assay
R1453	T2380	T2368	punct	),Assay
R1454	T2381	T2355	punct	.,determined
R1455	T2383	T2384	advmod	Further,assessed
R1456	T2385	T2384	punct	", ",assessed
R1457	T2386	T2387	det	the,quality
R1458	T2387	T2384	nsubjpass	quality,assessed
R1459	T2388	T2387	prep	of,quality
R146	T398	T399	det	the,cochlea
R1460	T2389	T2390	compound	RNA,preparations
R1461	T2390	T2388	pobj	preparations,of
R1462	T2391	T2390	acl	obtained,preparations
R1463	T2392	T2391	prep	from,obtained
R1464	T2393	T2394	compound	stria,vascularis
R1465	T2394	T2392	pobj	vascularis,from
R1466	T2395	T2394	cc	and,vascularis
R1467	T2396	T2397	amod	spiral,ganglia
R1468	T2397	T2394	conj	ganglia,vascularis
R1469	T2398	T2384	auxpass	was,assessed
R147	T399	T397	pobj	cochlea,in
R1470	T2399	T2400	punct	(,Assay
R1471	T2400	T2384	parataxis	Assay,assessed
R1472	T2401	T2400	compound	Pico,Assay
R1473	T2402	T2400	punct	", ",Assay
R1474	T2403	T2404	nummod	2100,Bioanalyzer
R1475	T2404	T2400	appos	Bioanalyzer,Assay
R1476	T2405	T2400	punct	),Assay
R1477	T2406	T2384	punct	.,assessed
R1478	T2408	T2409	amod	Real,time
R1479	T2409	T2410	compound	time,PCR
R148	T400	T395	prep	in,found
R1480	T2410	T2413	nsubjpass	PCR,performed
R1481	T2411	T2410	compound	RT,PCR
R1482	T2412	T2410	punct	-,PCR
R1483	T2414	T2413	auxpass	was,performed
R1484	T2415	T2413	prep	on,performed
R1485	T2416	T2415	pobj	RNA,on
R1486	T2417	T2416	acl	obtained,RNA
R1487	T2418	T2417	prep	from,obtained
R1488	T2419	T2420	amod	individual,animals
R1489	T2420	T2418	pobj	animals,from
R149	T401	T402	amod	apical,membranes
R1490	T2421	T2422	punct	(,Cycler
R1491	T2422	T2417	parataxis	Cycler,obtained
R1492	T2423	T2424	compound	OneStep,PCR
R1493	T2424	T2422	dep	PCR,Cycler
R1494	T2425	T2424	compound	RT,PCR
R1495	T2426	T2424	punct	-,PCR
R1496	T2427	T2424	punct	", ",PCR
R1497	T2428	T2424	npadvmod	Qiagen,PCR
R1498	T2429	T2422	punct	;,Cycler
R1499	T2430	T2422	compound	Smart,Cycler
R15	T244	T235	conj	causes,abolishes
R150	T402	T400	pobj	membranes,in
R1500	T2431	T2422	punct	", ",Cycler
R1501	T2432	T2422	npadvmod	Cepheid,Cycler
R1502	T2433	T2422	punct	", ",Cycler
R1503	T2434	T2422	npadvmod	Sunnyvale,Cycler
R1504	T2435	T2422	punct	", ",Cycler
R1505	T2436	T2422	npadvmod	CA,Cycler
R1506	T2437	T2422	punct	),Cycler
R1507	T2438	T2413	prep	in,performed
R1508	T2439	T2440	det	the,presence
R1509	T2440	T2438	pobj	presence,in
R151	T403	T402	prep	of,membranes
R1510	T2441	T2440	prep	of,presence
R1511	T2442	T2443	nummod	0.2,SYBR
R1512	T2443	T2445	nmod	SYBR,I
R1513	T2444	T2443	punct	×,SYBR
R1514	T2445	T2441	pobj	I,of
R1515	T2446	T2445	amod	green,I
R1516	T2447	T2448	punct	(,Probes
R1517	T2448	T2445	parataxis	Probes,I
R1518	T2449	T2448	compound	Molecular,Probes
R1519	T2450	T2448	punct	),Probes
R152	T404	T405	amod	spiral,cells
R1520	T2451	T2413	punct	.,performed
R1521	T2453	T2454	nsubjpass	Transcripts,amplified
R1522	T2455	T2453	prep	of,Transcripts
R1523	T2456	T2457	compound	18S,rRNA
R1524	T2457	T2455	pobj	rRNA,of
R1525	T2458	T2457	cc	and,rRNA
R1526	T2459	T2457	conj	mRNA,rRNA
R1527	T2460	T2453	prep	for,Transcripts
R1528	T2461	T2462	det	the,channels
R1529	T2462	T2460	pobj	channels,for
R153	T405	T403	pobj	cells,of
R1530	T2463	T2462	nmod	K,channels
R1531	T2464	T2463	punct	+,K
R1532	T2465	T2462	appos	KCNJ10,channels
R1533	T2466	T2465	punct	", ",KCNJ10
R1534	T2467	T2465	conj	KCNQ1,KCNJ10
R1535	T2468	T2467	cc	and,KCNQ1
R1536	T2469	T2467	conj	KCNQ4,KCNQ1
R1537	T2470	T2454	auxpass	were,amplified
R1538	T2471	T2454	advcl	using,amplified
R1539	T2472	T2473	npadvmod	gene,specific
R154	T406	T405	compound	prominence,cells
R1540	T2473	T2475	amod	specific,primers
R1541	T2474	T2473	punct	-,specific
R1542	T2475	T2471	dobj	primers,using
R1543	T2476	T2477	punct	(,Table
R1544	T2477	T2454	parataxis	Table,amplified
R1545	T2478	T2477	nummod	1,Table
R1546	T2479	T2477	punct	),Table
R1547	T2480	T2454	punct	.,amplified
R1548	T2482	T2483	nsubjpass	RT,performed
R1549	T2484	T2483	auxpass	was,performed
R155	T407	T402	cc	and,membranes
R1550	T2485	T2483	prep	for,performed
R1551	T2486	T2487	nummod	30,min
R1552	T2487	T2485	pobj	min,for
R1553	T2488	T2483	prep	at,performed
R1554	T2489	T2490	nummod	50,°C
R1555	T2490	T2488	pobj	°C,at
R1556	T2491	T2483	cc	and,performed
R1557	T2492	T2493	nummod	15,min
R1558	T2493	T2483	conj	min,performed
R1559	T2494	T2493	prep	at,min
R156	T408	T409	npadvmod	spindle,shaped
R1560	T2495	T2496	nummod	95,°C
R1561	T2496	T2494	pobj	°C,at
R1562	T2497	T2483	punct	.,performed
R1563	T2499	T2500	nsubj	PCR,consisted
R1564	T2501	T2500	prep	of,consisted
R1565	T2502	T2503	nummod	50,cycles
R1566	T2503	T2501	pobj	cycles,of
R1567	T2504	T2503	prep	of,cycles
R1568	T2505	T2506	nummod	1,min
R1569	T2506	T2504	pobj	min,of
R157	T409	T411	amod	shaped,cells
R1570	T2507	T2506	prep	at,min
R1571	T2508	T2509	nummod	60,°C
R1572	T2509	T2507	pobj	°C,at
R1573	T2510	T2506	punct	", ",min
R1574	T2511	T2512	nummod	1,min
R1575	T2512	T2506	conj	min,min
R1576	T2513	T2512	prep	at,min
R1577	T2514	T2515	nummod	72,°C
R1578	T2515	T2513	pobj	°C,at
R1579	T2516	T2512	punct	", ",min
R158	T410	T409	punct	-,shaped
R1580	T2517	T2512	conj	7s,min
R1581	T2518	T2517	amod	heating,7s
R1582	T2519	T2517	prep	to,7s
R1583	T2520	T2521	amod	hot,measurement
R1584	T2521	T2523	compound	measurement,temperature
R1585	T2522	T2521	punct	-,measurement
R1586	T2523	T2519	pobj	temperature,to
R1587	T2524	T2517	punct	", ",7s
R1588	T2525	T2526	compound	13s,measurement
R1589	T2526	T2517	conj	measurement,7s
R159	T411	T402	conj	cells,membranes
R1590	T2527	T2526	compound	hot,measurement
R1591	T2528	T2526	punct	-,measurement
R1592	T2529	T2526	prep	at,measurement
R1593	T2530	T2531	quantmod	3,5
R1594	T2531	T2533	nummod	5,°C
R1595	T2532	T2531	punct	–,5
R1596	T2533	T2534	npadvmod	°C,below
R1597	T2534	T2529	prep	below,at
R1598	T2535	T2536	compound	product,temperature
R1599	T2536	T2534	pobj	temperature,below
R16	T245	T244	dobj	deafness,causes
R160	T412	T411	prep	of,cells
R1600	T2537	T2536	compound	melting,temperature
R1601	T2538	T2539	punct	(,Table
R1602	T2539	T2536	parataxis	Table,temperature
R1603	T2540	T2539	nummod	1,Table
R1604	T2541	T2539	punct	),Table
R1605	T2542	T2526	cc	and,measurement
R1606	T2543	T2544	nummod	1,min
R1607	T2544	T2526	conj	min,measurement
R1608	T2545	T2544	prep	at,min
R1609	T2546	T2547	nummod	94,°C
R161	T413	T414	compound	stria,vascularis
R1610	T2547	T2545	pobj	°C,at
R1611	T2548	T2500	punct	.,consisted
R1612	T2550	T2551	amod	Hot,measurements
R1613	T2551	T2553	nsubjpass	measurements,performed
R1614	T2552	T2551	punct	-,measurements
R1615	T2554	T2553	auxpass	were,performed
R1616	T2555	T2556	aux	to,eliminate
R1617	T2556	T2553	advcl	eliminate,performed
R1618	T2557	T2558	det	the,detection
R1619	T2558	T2556	dobj	detection,eliminate
R162	T414	T412	pobj	vascularis,of
R1620	T2559	T2558	prep	of,detection
R1621	T2560	T2561	compound	primer,dimers
R1622	T2561	T2559	pobj	dimers,of
R1623	T2562	T2561	punct	-,dimers
R1624	T2563	T2564	dep	that,had
R1625	T2564	T2561	relcl	had,dimers
R1626	T2565	T2566	compound	melting,temperatures
R1627	T2566	T2564	dobj	temperatures,had
R1628	T2567	T2564	prep	between,had
R1629	T2568	T2569	nummod	72,°C
R163	T415	T395	punct	", ",found
R1630	T2569	T2567	pobj	°C,between
R1631	T2570	T2568	cc	and,72
R1632	T2571	T2568	conj	75,72
R1633	T2572	T2573	punct	[,15
R1634	T2573	T2564	parataxis	15,had
R1635	T2574	T2573	punct	],15
R1636	T2575	T2553	punct	.,performed
R1637	T2577	T2578	nsubjpass	PCR,followed
R1638	T2579	T2578	auxpass	was,followed
R1639	T2580	T2578	agent	by,followed
R164	T416	T395	prep	in,found
R1640	T2581	T2582	det	a,melt
R1641	T2582	T2580	pobj	melt,by
R1642	T2583	T2584	punct	(,°C
R1643	T2584	T2582	parataxis	°C,melt
R1644	T2585	T2586	quantmod	60,95
R1645	T2586	T2584	nummod	95,°C
R1646	T2587	T2586	quantmod	to,95
R1647	T2588	T2584	punct	),°C
R1648	T2589	T2578	punct	.,followed
R1649	T2591	T2592	det	The,generation
R165	T417	T418	amod	outer,cells
R1650	T2592	T2593	nsubjpass	generation,checked
R1651	T2594	T2592	prep	of,generation
R1652	T2595	T2596	det	a,product
R1653	T2596	T2594	pobj	product,of
R1654	T2597	T2596	amod	single,product
R1655	T2598	T2596	prep	of,product
R1656	T2599	T2600	amod	appropriate,size
R1657	T2600	T2598	pobj	size,of
R1658	T2601	T2593	auxpass	was,checked
R1659	T2602	T2593	advmod	routinely,checked
R166	T418	T416	pobj	cells,in
R1660	T2603	T2593	prep	by,checked
R1661	T2604	T2605	det	the,presence
R1662	T2605	T2603	pobj	presence,by
R1663	T2606	T2605	prep	of,presence
R1664	T2607	T2608	det	a,peak
R1665	T2608	T2606	pobj	peak,of
R1666	T2609	T2608	amod	single,peak
R1667	T2610	T2608	compound	melt,peak
R1668	T2611	T2603	cc	and,by
R1669	T2612	T2603	conj	by,by
R167	T419	T418	nmod	sulcus,cells
R1670	T2613	T2614	compound	agarose,gel
R1671	T2614	T2615	compound	gel,electrophoresis
R1672	T2615	T2612	pobj	electrophoresis,by
R1673	T2616	T2615	punct	-,electrophoresis
R1674	T2617	T2593	punct	.,checked
R1675	T2619	T2620	compound	Product,identity
R1676	T2620	T2621	nsubjpass	identity,confirmed
R1677	T2622	T2621	auxpass	was,confirmed
R1678	T2623	T2621	prep	by,confirmed
R1679	T2624	T2623	pobj	sequencing,by
R168	T420	T419	cc	and,sulcus
R1680	T2625	T2621	punct	.,confirmed
R1681	T2627	T2628	compound	"Table 1 Primers Template",molecules
R1682	T2628	T2629	nsubjpass	molecules,quantified
R1683	T2630	T2628	punct	(,molecules
R1684	T2631	T2628	appos	T,molecules
R1685	T2632	T2628	punct	),molecules
R1686	T2633	T2629	auxpass	were,quantified
R1687	T2634	T2629	prep	according,quantified
R1688	T2635	T2634	prep	to,according
R1689	T2636	T2637	nsubj	T,P
R169	T421	T419	conj	root,sulcus
R1690	T2637	T2635	pcomp	P,to
R1691	T2638	T2637	punct	=,P
R1692	T2639	T2640	punct	/,Ct
R1693	T2640	T2637	prep	Ct,P
R1694	T2641	T2640	punct	(,Ct
R1695	T2642	T2640	nmod	F,Ct
R1696	T2643	T2640	punct	^,Ct
R1697	T2644	T2640	punct	),Ct
R1698	T2645	T2646	advmod	where,is
R1699	T2646	T2637	advcl	is,P
R17	T246	T244	prep	in,causes
R170	T422	T395	punct	.,found
R1700	T2647	T2646	nsubj	P,is
R1701	T2648	T2649	det	the,number
R1702	T2649	T2646	attr	number,is
R1703	T2650	T2649	prep	of,number
R1704	T2651	T2652	compound	product,molecules
R1705	T2652	T2650	pobj	molecules,of
R1706	T2653	T2646	punct	", ",is
R1707	T2654	T2655	nsubj	F,is
R1708	T2655	T2646	conj	is,is
R1709	T2656	T2657	det	the,fidelity
R171	T424	T425	compound	Endolymph,volume
R1710	T2657	T2655	attr	fidelity,is
R1711	T2658	T2657	prep	of,fidelity
R1712	T2659	T2660	det	the,reaction
R1713	T2660	T2658	pobj	reaction,of
R1714	T2661	T2655	cc	and,is
R1715	T2662	T2663	nsubj	Ct,is
R1716	T2663	T2655	conj	is,is
R1717	T2664	T2665	det	the,cycle
R1718	T2665	T2663	attr	cycle,is
R1719	T2666	T2667	prep	at,reaches
R172	T425	T426	nsubjpass	volume,increased
R1720	T2667	T2665	relcl	reaches,cycle
R1721	T2668	T2666	pobj	which,at
R1722	T2669	T2670	det	the,number
R1723	T2670	T2667	nsubj	number,reaches
R1724	T2671	T2670	prep	of,number
R1725	T2672	T2673	compound	product,molecules
R1726	T2673	T2671	pobj	molecules,of
R1727	T2674	T2675	det	a,threshold
R1728	T2675	T2667	dobj	threshold,reaches
R1729	T2676	T2675	amod	chosen,threshold
R173	T427	T425	prep	in,volume
R1730	T2677	T2678	punct	[,15
R1731	T2678	T2646	parataxis	15,is
R1732	T2679	T2678	punct	],15
R1733	T2680	T2629	punct	.,quantified
R1734	T2682	T2683	nsubjpass	Fidelity,obtained
R1735	T2684	T2682	punct	(,Fidelity
R1736	T2685	T2682	appos	F,Fidelity
R1737	T2686	T2682	punct	),Fidelity
R1738	T2687	T2683	auxpass	was,obtained
R1739	T2688	T2683	prep	from,obtained
R174	T428	T429	nmod	Slc26a4,mice
R1740	T2689	T2690	det	the,slope
R1741	T2690	T2688	pobj	slope,from
R1742	T2691	T2690	punct	(,slope
R1743	T2692	T2690	appos	S,slope
R1744	T2693	T2690	punct	),slope
R1745	T2694	T2690	prep	of,slope
R1746	T2695	T2696	det	the,phase
R1747	T2696	T2694	pobj	phase,of
R1748	T2697	T2698	npadvmod	log,linear
R1749	T2698	T2696	amod	linear,phase
R175	T429	T427	pobj	mice,in
R1750	T2699	T2698	punct	-,linear
R1751	T2700	T2696	prep	of,phase
R1752	T2701	T2702	det	the,curve
R1753	T2702	T2700	pobj	curve,of
R1754	T2703	T2702	compound	growth,curve
R1755	T2704	T2683	prep	via,obtained
R1756	T2705	T2706	det	a,polynomial
R1757	T2706	T2704	pobj	polynomial,via
R1758	T2707	T2708	amod	best,fit
R1759	T2708	T2706	nmod	fit,polynomial
R176	T430	T428	punct	-,Slc26a4
R1760	T2709	T2708	punct	-,fit
R1761	T2710	T2711	amod	fifth,order
R1762	T2711	T2706	compound	order,polynomial
R1763	T2712	T2711	punct	-,order
R1764	T2713	T2706	punct	: ,polynomial
R1765	T2714	T2715	nsubj	F,7.39
R1766	T2715	T2706	appos	7.39,polynomial
R1767	T2716	T2715	punct	=,7.39
R1768	T2717	T2715	punct	+,7.39
R1769	T2718	T2719	nummod	3.80,S
R177	T431	T428	punct	/,Slc26a4
R1770	T2719	T2715	appos	S,7.39
R1771	T2720	T2719	punct	×,S
R1772	T2721	T2715	punct	+,7.39
R1773	T2722	T2723	nummod	1.05,S2
R1774	T2723	T2715	appos	S2,7.39
R1775	T2724	T2723	punct	×,S2
R1776	T2725	T2715	punct	+,7.39
R1777	T2726	T2727	nummod	0.15,S3
R1778	T2727	T2715	appos	S3,7.39
R1779	T2728	T2727	punct	×,S3
R178	T432	T428	punct	-,Slc26a4
R1780	T2729	T2715	punct	+,7.39
R1781	T2730	T2731	quantmod	11.38,3
R1782	T2731	T2735	nummod	3,S4
R1783	T2732	T2731	punct	×,3
R1784	T2733	T2731	compound	10,3
R1785	T2734	T2731	punct	-,3
R1786	T2735	T2715	appos	S4,7.39
R1787	T2736	T2735	punct	*,S4
R1788	T2737	T2715	punct	+,7.39
R1789	T2738	T2739	quantmod	3.39,4
R179	T433	T426	auxpass	was,increased
R1790	T2739	T2743	nummod	4,S5
R1791	T2740	T2739	punct	×,4
R1792	T2741	T2739	compound	10,4
R1793	T2742	T2739	punct	-,4
R1794	T2743	T2715	appos	S5,7.39
R1795	T2744	T2743	punct	×,S5
R1796	T2745	T2683	punct	.,obtained
R1797	T2747	T2748	det	The,number
R1798	T2748	T2749	nsubjpass	number,determined
R1799	T2750	T2748	prep	of,number
R18	T247	T248	compound	Pendred,syndrome
R180	T434	T426	cc	and,increased
R1800	T2751	T2752	compound	product,molecules
R1801	T2752	T2750	pobj	molecules,of
R1802	T2753	T2752	prep	at,molecules
R1803	T2754	T2753	pobj	threshold,at
R1804	T2755	T2752	punct	(,molecules
R1805	T2756	T2752	appos	PCt,molecules
R1806	T2757	T2752	punct	),molecules
R1807	T2758	T2749	auxpass	was,determined
R1808	T2759	T2749	prep	by,determined
R1809	T2760	T2759	pobj	amplification,by
R181	T435	T436	compound	tissue,masses
R1810	T2761	T2760	prep	of,amplification
R1811	T2762	T2763	amod	known,amounts
R1812	T2763	T2761	pobj	amounts,of
R1813	T2764	T2763	prep	of,amounts
R1814	T2765	T2766	compound	18S,rRNA
R1815	T2766	T2764	pobj	rRNA,of
R1816	T2767	T2760	prep	according,amplification
R1817	T2768	T2767	prep	to,according
R1818	T2769	T2770	nsubj	PCt,T
R1819	T2770	T2768	pcomp	T,to
R182	T436	T437	nsubjpass	masses,reduced
R1820	T2771	T2770	punct	=,T
R1821	T2772	T2770	punct	×,T
R1822	T2773	T2774	nmod	F,Ct
R1823	T2774	T2770	appos	Ct,T
R1824	T2775	T2774	punct	^,Ct
R1825	T2776	T2749	punct	.,determined
R1826	T2778	T2779	nsubjpass	Quantifications,used
R1827	T2780	T2778	prep	of,Quantifications
R1828	T2781	T2782	compound	18S,rRNA
R1829	T2782	T2780	pobj	rRNA,of
R183	T437	T426	conj	reduced,increased
R1830	T2783	T2779	auxpass	were,used
R1831	T2784	T2785	aux	to,compare
R1832	T2785	T2779	advcl	compare,used
R1833	T2786	T2787	compound	tissue,amounts
R1834	T2787	T2785	dobj	amounts,compare
R1835	T2788	T2779	punct	.,used
R1836	T2790	T2791	amod	Genomic,contamination
R1837	T2791	T2792	nsubjpass	contamination,assessed
R1838	T2793	T2791	prep	of,contamination
R1839	T2794	T2795	amod	inner,ear
R184	T438	T436	prep	in,masses
R1840	T2795	T2796	compound	ear,samples
R1841	T2796	T2793	pobj	samples,of
R1842	T2797	T2792	auxpass	was,assessed
R1843	T2798	T2799	aux	to,be
R1844	T2799	T2792	xcomp	be,assessed
R1845	T2800	T2801	punct	<,%
R1846	T2801	T2799	acomp	%,be
R1847	T2802	T2801	nummod	0.02,%
R1848	T2803	T2792	prep	by,assessed
R1849	T2804	T2803	pobj	omission,by
R185	T439	T438	pobj	areas,in
R1850	T2805	T2804	prep	of,omission
R1851	T2806	T2807	det	the,step
R1852	T2807	T2805	pobj	step,of
R1853	T2808	T2807	compound	RT,step
R1854	T2809	T2792	punct	.,assessed
R1855	T2833	T2834	advmod	In,vitro
R1856	T2834	T2835	amod	vitro,electrophysiology
R1857	T2837	T2838	nsubjpass	Animals,anesthetized
R1858	T2839	T2838	auxpass	were,anesthetized
R1859	T2840	T2838	advmod	deeply,anesthetized
R186	T440	T441	advmod	normally,occupied
R1860	T2841	T2838	prep	with,anesthetized
R1861	T2842	T2843	compound	sodium,pentobarbital
R1862	T2843	T2841	pobj	pentobarbital,with
R1863	T2844	T2845	punct	(,mg
R1864	T2845	T2838	parataxis	mg,anesthetized
R1865	T2846	T2845	nummod	100,mg
R1866	T2847	T2848	punct	/,kg
R1867	T2848	T2845	prep	kg,mg
R1868	T2849	T2845	advmod	i.p.,mg
R1869	T2850	T2845	punct	),mg
R187	T441	T439	acl	occupied,areas
R1870	T2851	T2838	punct	.,anesthetized
R1871	T2853	T2854	compound	Stria,vascularis
R1872	T2854	T2855	nsubjpass	vascularis,obtained
R1873	T2856	T2854	prep	without,vascularis
R1874	T2857	T2858	amod	spiral,ligament
R1875	T2858	T2856	pobj	ligament,without
R1876	T2859	T2855	auxpass	was,obtained
R1877	T2860	T2855	prep	by,obtained
R1878	T2861	T2860	pobj	microdissection,by
R1879	T2862	T2855	punct	.,obtained
R188	T442	T441	agent	by,occupied
R1880	T2864	T2865	nsubjpass	Currents,recorded
R1881	T2866	T2864	acl	generated,Currents
R1882	T2867	T2866	agent	by,generated
R1883	T2868	T2869	det	the,epithelium
R1884	T2869	T2867	pobj	epithelium,by
R1885	T2870	T2869	nmod	stria,epithelium
R1886	T2871	T2869	amod	marginal,epithelium
R1887	T2872	T2865	auxpass	were,recorded
R1888	T2873	T2874	punct	[,16
R1889	T2874	T2865	parataxis	16,recorded
R189	T443	T444	nmod	type,I
R1890	T2875	T2874	punct	],16
R1891	T2876	T2865	punct	.,recorded
R1892	T2878	T2879	det	A,microelectrode
R1893	T2879	T2884	nsubjpass	microelectrode,positioned
R1894	T2880	T2881	compound	Pt,Ir
R1895	T2881	T2879	compound	Ir,microelectrode
R1896	T2882	T2881	punct	-,Ir
R1897	T2883	T2879	compound	wire,microelectrode
R1898	T2885	T2879	prep	with,microelectrode
R1899	T2886	T2887	det	a,tip
R19	T248	T249	compound	syndrome,model
R190	T444	T445	nummod	I,fibrocytes
R1900	T2887	T2885	pobj	tip,with
R1901	T2888	T2887	nmod	Pt,tip
R1902	T2889	T2888	punct	-,Pt
R1903	T2890	T2888	amod	black,Pt
R1904	T2891	T2884	auxpass	was,positioned
R1905	T2892	T2893	compound	20,30
R1906	T2893	T2895	nummod	30,μm
R1907	T2894	T2893	punct	–,30
R1908	T2895	T2896	npadvmod	μm,from
R1909	T2896	T2884	prep	from,positioned
R191	T445	T442	pobj	fibrocytes,by
R1910	T2897	T2898	det	the,surface
R1911	T2898	T2896	pobj	surface,from
R1912	T2899	T2898	amod	apical,surface
R1913	T2900	T2898	prep	of,surface
R1914	T2901	T2902	det	the,epithelium
R1915	T2902	T2900	pobj	epithelium,of
R1916	T2903	T2884	cc	and,positioned
R1917	T2904	T2884	conj	vibrated,positioned
R1918	T2905	T2904	prep	at,vibrated
R1919	T2906	T2907	compound	200,400
R192	T446	T444	cc	and,I
R1920	T2907	T2909	nummod	400,Hz
R1921	T2908	T2907	punct	–,400
R1922	T2909	T2905	pobj	Hz,at
R1923	T2910	T2904	agent	by,vibrated
R1924	T2911	T2912	amod	piezo,electric
R1925	T2912	T2914	amod	electric,elements
R1926	T2913	T2912	punct	-,electric
R1927	T2914	T2910	pobj	elements,by
R1928	T2915	T2914	compound	bimorph,elements
R1929	T2916	T2917	punct	(,version
R193	T447	T444	conj	II,I
R1930	T2917	T2914	parataxis	version,elements
R1931	T2918	T2919	compound	Applicable,Electronics
R1932	T2919	T2917	dep	Electronics,version
R1933	T2920	T2919	punct	", ",Electronics
R1934	T2921	T2922	compound	Forest,Dale
R1935	T2922	T2919	npadvmod	Dale,Electronics
R1936	T2923	T2919	punct	", ",Electronics
R1937	T2924	T2919	npadvmod	MA,Electronics
R1938	T2925	T2917	punct	;,version
R1939	T2926	T2917	compound	ASET,version
R194	T448	T437	auxpass	were,reduced
R1940	T2927	T2917	nummod	1.05,version
R1941	T2928	T2917	punct	", ",version
R1942	T2929	T2930	compound	Science,Wares
R1943	T2930	T2917	npadvmod	Wares,version
R1944	T2931	T2917	punct	", ",version
R1945	T2932	T2933	compound	East,Falmouth
R1946	T2933	T2917	npadvmod	Falmouth,version
R1947	T2934	T2917	punct	", ",version
R1948	T2935	T2917	npadvmod	MA,version
R1949	T2936	T2917	punct	),version
R195	T449	T437	punct	.,reduced
R1950	T2937	T2884	punct	.,positioned
R1951	T2939	T2940	det	A,electrode
R1952	T2940	T2944	nsubj	electrode,served
R1953	T2941	T2940	nmod	Pt,electrode
R1954	T2942	T2941	punct	-,Pt
R1955	T2943	T2941	amod	black,Pt
R1956	T2945	T2946	punct	(,gauge
R1957	T2946	T2940	parataxis	gauge,electrode
R1958	T2947	T2946	nummod	26,gauge
R1959	T2948	T2946	punct	-,gauge
R196	T451	T452	nmod	Slc26a4,mice
R1960	T2949	T2946	punct	),gauge
R1961	T2950	T2944	prep	as,served
R1962	T2951	T2950	pobj	reference,as
R1963	T2952	T2944	prep	in,served
R1964	T2953	T2954	det	the,chamber
R1965	T2954	T2952	pobj	chamber,in
R1966	T2955	T2954	compound	bath,chamber
R1967	T2956	T2944	punct	.,served
R1968	T2958	T2959	det	The,signals
R1969	T2959	T2960	nsubjpass	signals,digitized
R197	T452	T456	nsubj	mice,lacked
R1970	T2961	T2959	prep	from,signals
R1971	T2962	T2963	det	the,detectors
R1972	T2963	T2961	pobj	detectors,from
R1973	T2964	T2965	npadvmod	phase,sensitive
R1974	T2965	T2963	amod	sensitive,detectors
R1975	T2966	T2965	punct	-,sensitive
R1976	T2967	T2960	auxpass	were,digitized
R1977	T2968	T2969	punct	(,bit
R1978	T2969	T2960	parataxis	bit,digitized
R1979	T2970	T2969	nummod	16,bit
R198	T453	T451	punct	-,Slc26a4
R1980	T2971	T2969	punct	),bit
R1981	T2972	T2960	prep	at,digitized
R1982	T2973	T2974	det	a,rate
R1983	T2974	T2972	pobj	rate,at
R1984	T2975	T2974	prep	of,rate
R1985	T2976	T2977	nummod	0.5,Hz
R1986	T2977	T2975	pobj	Hz,of
R1987	T2978	T2960	punct	.,digitized
R1988	T2980	T2981	det	The,output
R1989	T2981	T2982	nsubjpass	output,expressed
R199	T454	T451	punct	/,Slc26a4
R1990	T2983	T2982	auxpass	was,expressed
R1991	T2984	T2982	prep	as,expressed
R1992	T2985	T2986	amod	current,density
R1993	T2986	T2984	pobj	density,as
R1994	T2987	T2986	prep	at,density
R1995	T2988	T2989	det	the,electrode
R1996	T2989	T2987	pobj	electrode,at
R1997	T2990	T2982	punct	.,expressed
R1998	T3050	T3051	advmod	In,situ
R1999	T3051	T3052	amod	situ,electrophysiology
R20	T249	T246	pobj	model,in
R200	T455	T451	punct	-,Slc26a4
R2000	T3054	T3055	nsubjpass	Animals,anesthetized
R2001	T3056	T3055	auxpass	were,anesthetized
R2002	T3057	T3055	prep	with,anesthetized
R2003	T3058	T3057	pobj	inactin,with
R2004	T3059	T3060	punct	(,mg
R2005	T3060	T3058	parataxis	mg,inactin
R2006	T3061	T3062	compound	thiobutabarbital,salt
R2007	T3062	T3060	dep	salt,mg
R2008	T3063	T3062	compound	sodium,salt
R2009	T3064	T3060	punct	", ",mg
R201	T457	T458	det	the,potential
R2010	T3065	T3060	nummod	140,mg
R2011	T3066	T3067	punct	/,kg
R2012	T3067	T3060	prep	kg,mg
R2013	T3068	T3060	advmod	ip,mg
R2014	T3069	T3060	punct	),mg
R2015	T3070	T3055	punct	.,anesthetized
R2016	T3072	T3073	det	The,potential
R2017	T3073	T3075	nsubjpass	potential,measured
R2018	T3074	T3073	amod	endocochlear,potential
R2019	T3076	T3073	cc	and,potential
R202	T458	T456	dobj	potential,lacked
R2020	T3077	T3078	det	the,K
R2021	T3078	T3073	conj	K,potential
R2022	T3079	T3078	amod	endolymphatic,K
R2023	T3080	T3078	punct	[,K
R2024	T3081	T3078	punct	+,K
R2025	T3082	T3078	punct	],K
R2026	T3083	T3075	auxpass	were,measured
R2027	T3084	T3075	prep	with,measured
R2028	T3085	T3086	amod	double,barreled
R2029	T3086	T3088	amod	barreled,microelectrodes
R203	T459	T458	amod	endocochlear,potential
R2030	T3087	T3086	punct	-,barreled
R2031	T3088	T3084	pobj	microelectrodes,with
R2032	T3089	T3090	punct	[,17
R2033	T3090	T3075	parataxis	17,measured
R2034	T3091	T3090	punct	],17
R2035	T3092	T3075	punct	.,measured
R2036	T3094	T3095	nsubjpass	Measurements,made
R2037	T3096	T3095	auxpass	were,made
R2038	T3097	T3095	prep	in,made
R2039	T3098	T3099	det	the,turn
R204	T460	T458	punct	", ",potential
R2040	T3099	T3097	pobj	turn,in
R2041	T3100	T3099	amod	basal,turn
R2042	T3101	T3095	prep	by,made
R2043	T3102	T3103	det	a,approach
R2044	T3103	T3101	pobj	approach,by
R2045	T3104	T3105	amod	round,window
R2046	T3105	T3103	compound	window,approach
R2047	T3106	T3105	punct	-,window
R2048	T3107	T3103	prep	through,approach
R2049	T3108	T3109	det	the,membrane
R205	T461	T462	dep	which,generated
R2050	T3109	T3107	pobj	membrane,through
R2051	T3110	T3109	amod	basilar,membrane
R2052	T3111	T3107	cc	and,through
R2053	T3112	T3107	conj	in,through
R2054	T3113	T3114	det	the,turn
R2055	T3114	T3112	pobj	turn,in
R2056	T3115	T3114	amod	apical,turn
R2057	T3116	T3117	mark	after,thinning
R2058	T3117	T3095	advcl	thinning,made
R2059	T3118	T3119	det	the,bone
R206	T462	T458	relcl	generated,potential
R2060	T3119	T3117	dobj	bone,thinning
R2061	T3120	T3117	prep	over,thinning
R2062	T3121	T3122	det	the,vascularis
R2063	T3122	T3120	pobj	vascularis,over
R2064	T3123	T3122	compound	stria,vascularis
R2065	T3124	T3117	cc	and,thinning
R2066	T3125	T3117	conj	picking,thinning
R2067	T3126	T3127	det	a,hole
R2068	T3127	T3125	dobj	hole,picking
R2069	T3128	T3127	amod	small,hole
R207	T463	T462	auxpass	is,generated
R2070	T3129	T3130	punct	(,μm
R2071	T3130	T3127	parataxis	μm,hole
R2072	T3131	T3132	punct	~,30
R2073	T3132	T3130	nummod	30,μm
R2074	T3133	T3130	punct	),μm
R2075	T3134	T3095	punct	.,made
R2076	T3136	T3137	npadvmod	K,selective
R2077	T3137	T3140	amod	selective,electrodes
R2078	T3138	T3136	punct	+,K
R2079	T3139	T3137	punct	-,selective
R208	T464	T462	prep	across,generated
R2080	T3140	T3141	nsubjpass	electrodes,calibrated
R2081	T3142	T3141	auxpass	were,calibrated
R2082	T3143	T3141	prep	in,calibrated
R2083	T3144	T3143	pobj	solutions,in
R2084	T3145	T3144	prep	of,solutions
R2085	T3146	T3147	amod	constant,concentration
R2086	T3147	T3145	pobj	concentration,of
R2087	T3148	T3147	nmod	cation,concentration
R2088	T3149	T3150	punct	(,K
R2089	T3150	T3148	parataxis	K,cation
R209	T465	T466	det	the,barrier
R2090	T3151	T3150	punct	+,K
R2091	T3152	T3150	cc	and,K
R2092	T3153	T3150	conj	Na,K
R2093	T3154	T3153	punct	+,Na
R2094	T3155	T3150	punct	),K
R2095	T3156	T3144	prep	of,solutions
R2096	T3157	T3158	nummod	150,mM
R2097	T3158	T3156	pobj	mM,of
R2098	T3159	T3141	punct	.,calibrated
R2099	T3161	T3162	det	The,selectivity
R21	T250	T249	compound	mouse,model
R210	T466	T464	pobj	barrier,across
R2100	T3162	T3164	nsubj	selectivity,produced
R2101	T3163	T3162	amod	minor,selectivity
R2102	T3165	T3162	prep	of,selectivity
R2103	T3166	T3167	det	the,electrodes
R2104	T3167	T3165	pobj	electrodes,of
R2105	T3168	T3167	nmod	K,electrodes
R2106	T3169	T3168	punct	+,K
R2107	T3170	T3162	prep	for,selectivity
R2108	T3171	T3170	pobj	Na,for
R2109	T3172	T3171	punct	+,Na
R211	T467	T466	amod	basal,barrier
R2110	T3173	T3174	det	a,nonlinearity
R2111	T3174	T3164	dobj	nonlinearity,produced
R2112	T3175	T3174	prep	in,nonlinearity
R2113	T3176	T3177	det	the,curve
R2114	T3177	T3175	pobj	curve,in
R2115	T3178	T3177	compound	calibration,curve
R2116	T3179	T3174	punct	", ",nonlinearity
R2117	T3180	T3181	dep	which,fitted
R2118	T3181	T3174	relcl	fitted,nonlinearity
R2119	T3182	T3181	auxpass	was,fitted
R212	T468	T466	compound	cell,barrier
R2120	T3183	T3181	advmod	closely,fitted
R2121	T3184	T3181	prep	by,fitted
R2122	T3185	T3186	det	the,equation
R2123	T3186	T3184	pobj	equation,by
R2124	T3187	T3186	compound	Nicolski,equation
R2125	T3188	T3181	advcl	using,fitted
R2126	T3189	T3190	amod	nonlinear,software
R2127	T3190	T3188	dobj	software,using
R2128	T3191	T3190	nmod	curve,software
R2129	T3192	T3191	punct	-,curve
R213	T469	T462	agent	by,generated
R2130	T3193	T3191	amod	fitting,curve
R2131	T3194	T3195	punct	(,OriginLab
R2132	T3195	T3190	parataxis	OriginLab,software
R2133	T3196	T3195	punct	", ",OriginLab
R2134	T3197	T3195	npadvmod	Northampton,OriginLab
R2135	T3198	T3195	punct	", ",OriginLab
R2136	T3199	T3195	npadvmod	MA,OriginLab
R2137	T3200	T3195	punct	),OriginLab
R2138	T3201	T3174	punct	: ,nonlinearity
R2139	T3202	T3203	nsubj	V,K
R214	T470	T471	det	the,channel
R2140	T3203	T3174	appos	K,nonlinearity
R2141	T3204	T3203	punct	=,K
R2142	T3205	T3203	nmod	Vi,K
R2143	T3206	T3203	punct	+,K
R2144	T3207	T3203	nmod	S,K
R2145	T3208	T3203	punct	×,K
R2146	T3209	T3203	nmod	log,K
R2147	T3210	T3203	punct	(,K
R2148	T3211	T3203	punct	[,K
R2149	T3212	T3203	punct	+,K
R215	T471	T469	pobj	channel,by
R2150	T3213	T3203	punct	],K
R2151	T3214	T3203	punct	+,K
R2152	T3215	T3216	nmod	A,Na
R2153	T3216	T3203	appos	Na,K
R2154	T3217	T3216	punct	×,Na
R2155	T3218	T3216	punct	[,Na
R2156	T3219	T3216	punct	+,Na
R2157	T3220	T3203	punct	],K
R2158	T3221	T3203	punct	),K
R2159	T3222	T3203	punct	", ",K
R216	T472	T471	nmod	K,channel
R2160	T3223	T3224	advmod	where,is
R2161	T3224	T3203	advcl	is,K
R2162	T3225	T3224	nsubj	Vi,is
R2163	T3226	T3227	det	an,term
R2164	T3227	T3224	attr	term,is
R2165	T3228	T3227	amod	offset,term
R2166	T3229	T3224	punct	", ",is
R2167	T3230	T3231	nsubj	S,is
R2168	T3231	T3224	conj	is,is
R2169	T3232	T3231	attr	slope,is
R217	T473	T472	punct	+,K
R2170	T3233	T3231	punct	", ",is
R2171	T3234	T3235	nsubj	A,is
R2172	T3235	T3231	conj	is,is
R2173	T3236	T3235	attr	selectivity,is
R2174	T3237	T3235	punct	", ",is
R2175	T3238	T3235	cc	and,is
R2176	T3239	T3240	punct	[,Na
R2177	T3240	T3241	nsubj	Na,is
R2178	T3241	T3235	conj	is,is
R2179	T3242	T3240	punct	+,Na
R218	T474	T471	appos	KCNJ10,channel
R2180	T3243	T3240	punct	],Na
R2181	T3244	T3245	nmod	Na,concentration
R2182	T3245	T3241	attr	concentration,is
R2183	T3246	T3244	punct	+,Na
R2184	T3247	T3164	punct	.,produced
R2185	T3249	T3250	nsubjpass	Calibrations,made
R2186	T3251	T3250	auxpass	were,made
R2187	T3252	T3253	advmod	immediately,after
R2188	T3253	T3250	prep	after,made
R2189	T3254	T3253	pobj	withdrawal,after
R219	T475	T471	acl	localized,channel
R2190	T3255	T3254	prep	of,withdrawal
R2191	T3256	T3257	det	the,electrodes
R2192	T3257	T3255	pobj	electrodes,of
R2193	T3258	T3254	prep	from,withdrawal
R2194	T3259	T3260	det	the,cochlea
R2195	T3260	T3258	pobj	cochlea,from
R2196	T3261	T3250	punct	.,made
R2197	T3263	T3264	nmod	Plasma,concentrations
R2198	T3264	T3267	nsubjpass	concentrations,obtained
R2199	T3265	T3264	nmod	K,concentrations
R22	T255	T256	compound	Pendred,syndrome
R220	T476	T475	prep	in,localized
R2200	T3266	T3265	punct	+,K
R2201	T3268	T3267	auxpass	were,obtained
R2202	T3269	T3267	advcl	using,obtained
R2203	T3270	T3271	det	a,analyzer
R2204	T3271	T3269	dobj	analyzer,using
R2205	T3272	T3271	compound	blood,analyzer
R2206	T3273	T3274	punct	(,M
R2207	T3274	T3267	parataxis	M,obtained
R2208	T3275	T3274	compound	Stat,M
R2209	T3276	T3274	compound	Profile,M
R221	T477	T478	amod	intermediate,cells
R2210	T3277	T3274	punct	", ",M
R2211	T3278	T3279	compound	Nova,Biomedical
R2212	T3279	T3274	npadvmod	Biomedical,M
R2213	T3280	T3274	punct	", ",M
R2214	T3281	T3274	npadvmod	Waltham,M
R2215	T3282	T3274	punct	", ",M
R2216	T3283	T3274	npadvmod	MA,M
R2217	T3284	T3274	punct	),M
R2218	T3285	T3267	punct	.,obtained
R2219	T3287	T3288	nsubjpass	Data,presented
R222	T478	T476	pobj	cells,in
R2220	T3288	T3290	ccomp	presented,denotes
R2221	T3289	T3288	auxpass	are,presented
R2222	T3291	T3288	prep	as,presented
R2223	T3292	T3293	compound	mean,sem
R2224	T3293	T3291	pobj	sem,as
R2225	T3294	T3293	compound	±,sem
R2226	T3295	T3290	punct	;,denotes
R2227	T3296	T3290	nsubj	n,denotes
R2228	T3297	T3298	det	the,number
R2229	T3298	T3290	dobj	number,denotes
R223	T479	T456	punct	.,lacked
R2230	T3299	T3298	prep	of,number
R2231	T3300	T3299	pobj	experiments,of
R2232	T3301	T3290	punct	.,denotes
R2233	T3303	T3304	nsubjpass	Differences,considered
R2234	T3305	T3304	auxpass	were,considered
R2235	T3306	T3304	oprd	significant,considered
R2236	T3307	T3308	advmod	when,0.05
R2237	T3308	T3304	advcl	0.05,considered
R2238	T3309	T3308	nsubj	p,0.05
R2239	T3310	T3308	punct	<,0.05
R224	T481	T482	compound	Stria,vascularis
R2240	T3311	T3304	punct	.,considered
R225	T482	T483	nsubj	vascularis,was
R226	T484	T483	acomp	hyperpigmented,was
R2269	T4701	T4702	advmod	In,situ
R227	T485	T483	punct	", ",was
R2270	T4702	T4703	amod	situ,hybridization
R2271	T4703	T4704	nsubj	hybridization,suggested
R2272	T4705	T4703	prep	in,hybridization
R2273	T4706	T4707	det	the,cochlea
R2274	T4707	T4705	pobj	cochlea,in
R2275	T4708	T4704	aux	has,suggested
R2276	T4709	T4710	mark	that,expressed
R2277	T4710	T4704	ccomp	expressed,suggested
R2278	T4711	T4712	compound	pendrin,mRNA
R2279	T4712	T4710	nsubjpass	mRNA,expressed
R228	T486	T483	advcl	suggesting,was
R2280	T4713	T4710	auxpass	is,expressed
R2281	T4714	T4710	prep	in,expressed
R2282	T4715	T4714	pobj	cells,in
R2283	T4716	T4717	dep	that,reside
R2284	T4717	T4715	relcl	reside,cells
R2285	T4718	T4719	advmod	immediately,beneath
R2286	T4719	T4717	prep	beneath,reside
R2287	T4720	T4721	det	the,prominence
R2288	T4721	T4719	pobj	prominence,beneath
R2289	T4722	T4721	amod	spiral,prominence
R229	T487	T488	amod	unalleviated,damage
R2290	T4723	T4721	prep	on,prominence
R2291	T4724	T4725	det	the,wall
R2292	T4725	T4723	pobj	wall,on
R2293	T4726	T4725	amod	lateral,wall
R2294	T4727	T4725	prep	of,wall
R2295	T4728	T4729	det	the,sulcus
R2296	T4729	T4727	pobj	sulcus,of
R2297	T4730	T4729	amod	external,sulcus
R2298	T4731	T4732	punct	[,8
R2299	T4732	T4704	parataxis	8,suggested
R23	T256	T257	nsubjpass	syndrome,caused
R230	T488	T486	dobj	damage,suggesting
R2300	T4733	T4732	punct	],8
R2301	T4734	T4704	punct	.,suggested
R2302	T4736	T4737	aux	To,determine
R2303	T4737	T4738	advcl	determine,performed
R2304	T4739	T4740	det	the,location
R2305	T4740	T4737	dobj	location,determine
R2306	T4741	T4740	prep	of,location
R2307	T4742	T4743	compound	pendrin,expression
R2308	T4743	T4741	pobj	expression,of
R2309	T4744	T4743	compound	protein,expression
R231	T489	T490	amod	free,radical
R2310	T4745	T4738	punct	", ",performed
R2311	T4746	T4738	nsubj	we,performed
R2312	T4747	T4748	amod	confocal,immunocytochemistry
R2313	T4748	T4738	dobj	immunocytochemistry,performed
R2314	T4749	T4738	prep	on,performed
R2315	T4750	T4749	pobj	cryosections,on
R2316	T4751	T4750	acl	prepared,cryosections
R2317	T4752	T4751	prep	from,prepared
R2318	T4753	T4754	amod	temporal,bones
R2319	T4754	T4752	pobj	bones,from
R232	T490	T488	compound	radical,damage
R2320	T4755	T4754	prep	of,bones
R2321	T4756	T4757	amod	normal,mice
R2322	T4757	T4755	pobj	mice,of
R2323	T4758	T4757	punct	(,mice
R2324	T4759	T4757	nmod	Slc26a4,mice
R2325	T4760	T4759	punct	+,Slc26a4
R2326	T4761	T4759	punct	/,Slc26a4
R2327	T4762	T4759	punct	+,Slc26a4
R2328	T4763	T4757	punct	),mice
R2329	T4764	T4738	advcl	using,performed
R233	T491	T483	punct	.,was
R2330	T4765	T4766	det	an,antibody
R2331	T4766	T4764	dobj	antibody,using
R2332	T4767	T4766	amod	established,antibody
R2333	T4768	T4769	punct	[,3
R2334	T4769	T4738	parataxis	3,performed
R2335	T4770	T4769	punct	],3
R2336	T4771	T4738	punct	.,performed
R2337	T4773	T4774	nsubj	Staining,was
R2338	T4775	T4774	acomp	absent,was
R2339	T4776	T4777	advmod	when,pre-absorbed
R234	T493	T494	det	The,barrier
R2340	T4777	T4774	advcl	pre-absorbed,was
R2341	T4778	T4779	det	the,antibody
R2342	T4779	T4777	nsubjpass	antibody,pre-absorbed
R2343	T4780	T4779	amod	primary,antibody
R2344	T4781	T4777	auxpass	was,pre-absorbed
R2345	T4782	T4777	prep	with,pre-absorbed
R2346	T4783	T4784	det	the,peptide
R2347	T4784	T4782	pobj	peptide,with
R2348	T4785	T4784	amod	antigenic,peptide
R2349	T4786	T4787	punct	(,shown
R235	T494	T497	nsubj	barrier,appeared
R2350	T4787	T4777	parataxis	shown,pre-absorbed
R2351	T4788	T4787	nsubj	data,shown
R2352	T4789	T4787	neg	not,shown
R2353	T4790	T4787	punct	),shown
R2354	T4791	T4774	punct	.,was
R2355	T4793	T4794	amod	Strong,expression
R2356	T4794	T4795	nsubjpass	expression,observed
R2357	T4796	T4794	prep	of,expression
R2358	T4797	T4796	pobj	pendrin,of
R2359	T4798	T4795	auxpass	was,observed
R236	T495	T494	amod	basal,barrier
R2360	T4799	T4800	preconj	not,in
R2361	T4800	T4795	prep	in,observed
R2362	T4801	T4799	advmod	only,not
R2363	T4802	T4803	amod	outer,sulcus
R2364	T4803	T4804	nmod	sulcus,cells
R2365	T4804	T4800	pobj	cells,in
R2366	T4805	T4804	amod	epithelial,cells
R2367	T4806	T4807	punct	", ",predicted
R2368	T4807	T4804	parataxis	predicted,cells
R2369	T4808	T4807	mark	as,predicted
R237	T496	T494	compound	cell,barrier
R2370	T4809	T4807	prep	from,predicted
R2371	T4810	T4811	advmod	in,situ
R2372	T4811	T4812	amod	situ,data
R2373	T4812	T4809	pobj	data,from
R2374	T4813	T4812	compound	hybridization,data
R2375	T4814	T4807	punct	", ",predicted
R2376	T4815	T4800	cc	but,in
R2377	T4816	T4815	advmod	also,but
R2378	T4817	T4800	conj	in,in
R2379	T4818	T4819	compound	root,cells
R238	T497	T498	ccomp	appeared,were
R2380	T4819	T4817	pobj	cells,in
R2381	T4820	T4817	punct	", ",in
R2382	T4821	T4817	conj	in,in
R2383	T4822	T4823	amod	apical,membranes
R2384	T4823	T4821	pobj	membranes,in
R2385	T4824	T4823	prep	of,membranes
R2386	T4825	T4826	amod	spiral,prominence
R2387	T4826	T4827	nmod	prominence,cells
R2388	T4827	T4824	pobj	cells,of
R2389	T4828	T4827	nmod	surface,cells
R239	T499	T497	oprd	intact,appeared
R2390	T4829	T4827	amod	epithelial,cells
R2391	T4830	T4821	cc	and,in
R2392	T4831	T4821	conj	in,in
R2393	T4832	T4833	amod	apical,membranes
R2394	T4833	T4831	pobj	membranes,in
R2395	T4834	T4833	prep	of,membranes
R2396	T4835	T4836	npadvmod	spindle,shaped
R2397	T4836	T4838	amod	shaped,cells
R2398	T4837	T4836	punct	-,shaped
R2399	T4838	T4834	pobj	cells,of
R24	T258	T256	punct	", ",syndrome
R240	T500	T498	punct	;,were
R2400	T4839	T4840	dep	that,are
R2401	T4840	T4838	relcl	are,cells
R2402	T4841	T4840	attr	part,are
R2403	T4842	T4841	prep	of,part
R2404	T4843	T4844	compound	stria,vascularis
R2405	T4844	T4842	pobj	vascularis,of
R2406	T4845	T4846	punct	(,1c
R2407	T4846	T4795	parataxis	1c,observed
R2408	T4847	T4846	nmod	Fig.,1c
R2409	T4848	T4846	nummod	1a,1c
R241	T501	T502	amod	intermediate,cells
R2410	T4849	T4846	punct	",",1c
R2411	T4850	T4846	nummod	1b,1c
R2412	T4851	T4846	punct	",",1c
R2413	T4852	T4846	punct	),1c
R2414	T4853	T4795	punct	.,observed
R2415	T4855	T4856	det	The,presence
R2416	T4856	T4857	nsubj	presence,suggests
R2417	T4858	T4856	prep	of,presence
R2418	T4859	T4858	pobj	pendrin,of
R2419	T4860	T4856	prep	in,presence
R242	T502	T498	nsubj	cells,were
R2420	T4861	T4862	npadvmod	spindle,shaped
R2421	T4862	T4864	amod	shaped,cells
R2422	T4863	T4862	punct	-,shaped
R2423	T4864	T4860	pobj	cells,in
R2424	T4865	T4866	mark	that,secrete
R2425	T4866	T4857	ccomp	secrete,suggests
R2426	T4867	T4868	det	these,cells
R2427	T4868	T4866	nsubj	cells,secrete
R2428	T4869	T4866	dobj	HCO3,secrete
R2429	T4870	T4869	punct	-,HCO3
R243	T503	T502	cc	and,cells
R2430	T4871	T4866	prep	into,secrete
R2431	T4872	T4871	pobj	endolymph,into
R2432	T4873	T4857	punct	.,suggests
R2433	T4875	T4876	npadvmod	Pendrin,mediated
R2434	T4876	T4878	amod	mediated,transport
R2435	T4877	T4876	punct	-,mediated
R2436	T4878	T4881	nsubjpass	transport,shown
R2437	T4879	T4878	nmod	HCO3,transport
R2438	T4880	T4879	punct	-,HCO3
R2439	T4882	T4881	aux	has,shown
R244	T504	T505	compound	KCNJ10,mRNA
R2440	T4883	T4881	advmod	previously,shown
R2441	T4884	T4881	auxpass	been,shown
R2442	T4885	T4881	prep	in,shown
R2443	T4886	T4887	det	the,kidney
R2444	T4887	T4885	pobj	kidney,in
R2445	T4888	T4889	punct	[,9
R2446	T4889	T4881	parataxis	9,shown
R2447	T4890	T4889	punct	],9
R2448	T4891	T4881	punct	.,shown
R2449	T4893	T4894	nsubjpass	HCO3,generated
R245	T505	T502	conj	mRNA,cells
R2450	T4895	T4893	punct	-,HCO3
R2451	T4896	T4893	prep	in,HCO3
R2452	T4897	T4898	det	the,cochlea
R2453	T4898	T4896	pobj	cochlea,in
R2454	T4899	T4894	aux	may,generated
R2455	T4900	T4894	auxpass	be,generated
R2456	T4901	T4894	prep	from,generated
R2457	T4902	T4901	pobj	CO2,from
R2458	T4903	T4894	prep	by,generated
R2459	T4904	T4905	amod	carbonic,anhydrase
R246	T506	T498	acomp	present,were
R2460	T4905	T4903	pobj	anhydrase,by
R2461	T4906	T4905	acl	located,anhydrase
R2462	T4907	T4906	prep	in,located
R2463	T4908	T4909	amod	strial,cells
R2464	T4909	T4907	pobj	cells,in
R2465	T4910	T4909	amod	intermediate,cells
R2466	T4911	T4912	punct	[,19
R2467	T4912	T4894	parataxis	19,generated
R2468	T4913	T4912	nummod	18,19
R2469	T4914	T4912	punct	",",19
R247	T507	T498	cc	but,were
R2470	T4915	T4912	punct	],19
R2471	T4916	T4894	punct	.,generated
R2472	T4918	T4919	nsubjpass	CO2,supplied
R2473	T4920	T4919	aux	may,supplied
R2474	T4921	T4919	auxpass	be,supplied
R2475	T4922	T4919	agent	by,supplied
R2476	T4923	T4924	det	the,cells
R2477	T4924	T4922	pobj	cells,by
R2478	T4925	T4926	advmod	metabolically,active
R2479	T4926	T4924	amod	active,cells
R248	T508	T509	compound	KCNJ10,protein
R2480	T4927	T4926	advmod	highly,active
R2481	T4928	T4929	npadvmod	stria,marginal
R2482	T4929	T4924	amod	marginal,cells
R2483	T4930	T4919	punct	.,supplied
R2484	T4932	T4933	nsubj	It,is
R2485	T4934	T4933	acomp	conceivable,is
R2486	T4935	T4936	mark	that,interrupts
R2487	T4936	T4933	ccomp	interrupts,is
R2488	T4937	T4938	compound	pendrin,dysfunction
R2489	T4938	T4936	nsubj	dysfunction,interrupts
R249	T509	T510	nsubj	protein,was
R2490	T4939	T4940	nmod	HCO3,secretion
R2491	T4940	T4936	dobj	secretion,interrupts
R2492	T4941	T4939	punct	-,HCO3
R2493	T4942	T4936	cc	and,interrupts
R2494	T4943	T4936	conj	leads,interrupts
R2495	T4944	T4943	prep	to,leads
R2496	T4945	T4946	det	an,accumulation
R2497	T4946	T4944	pobj	accumulation,to
R2498	T4947	T4946	prep	of,accumulation
R2499	T4948	T4947	pobj	HCO3,of
R25	T259	T260	det	a,disorder
R250	T510	T498	conj	was,were
R2500	T4949	T4948	punct	-,HCO3
R2501	T4950	T4943	prep	in,leads
R2502	T4951	T4952	compound	stria,vascularis
R2503	T4952	T4950	pobj	vascularis,in
R2504	T4953	T4933	punct	.,is
R2505	T4955	T4956	amod	Preliminary,data
R2506	T4956	T4957	nsubj	data,support
R2507	T4958	T4959	punct	(,Wangemann
R2508	T4959	T4956	meta	Wangemann,data
R2509	T4960	T4959	nmod	et,Wangemann
R251	T511	T510	acomp	absent,was
R2510	T4961	T4959	nmod	al.,Wangemann
R2511	T4962	T4959	punct	", ",Wangemann
R2512	T4963	T4959	amod	unpublished,Wangemann
R2513	T4964	T4959	punct	),Wangemann
R2514	T4965	T4966	det	a,role
R2515	T4966	T4957	dobj	role,support
R2516	T4967	T4966	prep	for,role
R2517	T4968	T4967	pobj	pendrin,for
R2518	T4969	T4966	prep	in,role
R2519	T4970	T4971	nmod	HCO3,secretion
R252	T512	T498	punct	.,were
R2520	T4971	T4969	pobj	secretion,in
R2521	T4972	T4970	punct	-,HCO3
R2522	T4973	T4971	prep	into,secretion
R2523	T4974	T4973	pobj	endolymph,into
R2524	T4975	T4957	punct	.,support
R2525	T4982	T4983	nsubjpass	xtent,determined
R2526	T4984	T4982	prep	of,xtent
R2527	T4985	T4986	compound	pendrin,expression
R2528	T4986	T4984	pobj	expression,of
R2529	T4987	T4982	prep	in,xtent
R253	T514	T515	amod	Endolymphatic,concentrations
R2530	T4988	T4989	amod	spiral,cells
R2531	T4989	T4987	pobj	cells,in
R2532	T4990	T4989	compound	prominence,cells
R2533	T4991	T4989	cc	and,cells
R2534	T4992	T4993	compound	stria,vascularis
R2535	T4993	T4989	conj	vascularis,cells
R2536	T4994	T4983	auxpass	was,determined
R2537	T4995	T4983	prep	by,determined
R2538	T4996	T4995	pcomp	labeling,by
R2539	T4997	T4996	dobj	KCNQ1,labeling
R254	T515	T518	nsubj	concentrations,were
R2540	T4998	T4997	punct	", ",KCNQ1
R2541	T4999	T5000	det	a,channel
R2542	T5000	T4997	appos	channel,KCNQ1
R2543	T5001	T5000	nmod	K,channel
R2544	T5002	T5001	punct	+,K
R2545	T5003	T5004	dep	that,expressed
R2546	T5004	T5000	relcl	expressed,channel
R2547	T5005	T5004	auxpass	is,expressed
R2548	T5006	T5004	prep	in,expressed
R2549	T5007	T5008	amod	strial,marginal
R255	T516	T515	nmod	K,concentrations
R2550	T5008	T5009	amod	marginal,cells
R2551	T5009	T5006	pobj	cells,in
R2552	T5010	T4997	punct	", ",KCNQ1
R2553	T5011	T4997	cc	and,KCNQ1
R2554	T5012	T4997	conj	ZO,KCNQ1
R2555	T5013	T5012	punct	-,ZO
R2556	T5014	T5012	nummod	1,ZO
R2557	T5015	T5012	punct	", ",ZO
R2558	T5016	T5017	det	a,protein
R2559	T5017	T5012	appos	protein,ZO
R256	T517	T516	punct	+,K
R2560	T5018	T5019	amod	tight,junction
R2561	T5019	T5017	compound	junction,protein
R2562	T5020	T5021	dep	that,labels
R2563	T5021	T5017	relcl	labels,protein
R2564	T5022	T5023	amod	basal,cells
R2565	T5023	T5021	dobj	cells,labels
R2566	T5024	T5021	cc	and,labels
R2567	T5025	T5026	advmod	thereby,delineates
R2568	T5026	T5021	conj	delineates,labels
R2569	T5027	T5028	det	the,boundaries
R257	T519	T518	acomp	normal,were
R2570	T5028	T5026	dobj	boundaries,delineates
R2571	T5029	T5028	prep	of,boundaries
R2572	T5030	T5031	compound	stria,vascularis
R2573	T5031	T5029	pobj	vascularis,of
R2574	T5032	T4983	punct	.,determined
R2575	T5034	T5035	amod	Dual,experiments
R2576	T5035	T5037	nsubj	experiments,demonstrated
R2577	T5036	T5035	compound	labeling,experiments
R2578	T5038	T5039	mark	that,expressed
R2579	T5039	T5037	ccomp	expressed,demonstrated
R258	T520	T518	cc	and,were
R2580	T5040	T5039	nsubjpass	pendrin,expressed
R2581	T5041	T5039	auxpass	is,expressed
R2582	T5042	T5039	prep	in,expressed
R2583	T5043	T5044	npadvmod	spindle,shaped
R2584	T5044	T5046	amod	shaped,cells
R2585	T5045	T5044	punct	-,shaped
R2586	T5046	T5042	pobj	cells,in
R2587	T5047	T5046	punct	", ",cells
R2588	T5048	T5049	dep	which,are
R2589	T5049	T5046	relcl	are,cells
R259	T521	T522	compound	membrane,proteins
R2590	T5050	T5051	amod	surface,cells
R2591	T5051	T5049	attr	cells,are
R2592	T5052	T5051	amod	epithelial,cells
R2593	T5053	T5051	prep	in,cells
R2594	T5054	T5055	compound	stria,vascularis
R2595	T5055	T5053	pobj	vascularis,in
R2596	T5056	T5051	amod	adjacent,cells
R2597	T5057	T5056	prep	to,adjacent
R2598	T5058	T5059	amod	strial,cells
R2599	T5059	T5057	pobj	cells,to
R26	T260	T256	appos	disorder,syndrome
R260	T522	T523	nsubj	proteins,were
R2600	T5060	T5059	amod	marginal,cells
R2601	T5061	T5059	prep	near,cells
R2602	T5062	T5063	det	the,borders
R2603	T5063	T5061	pobj	borders,near
R2604	T5064	T5063	prep	of,borders
R2605	T5065	T5066	preconj	both,prominence
R2606	T5066	T5064	pobj	prominence,of
R2607	T5067	T5066	det	the,prominence
R2608	T5068	T5066	amod	spiral,prominence
R2609	T5069	T5066	cc	and,prominence
R261	T523	T518	conj	were,were
R2610	T5070	T5071	poss	Reissner,membrane
R2611	T5071	T5066	conj	membrane,prominence
R2612	T5072	T5070	case	's,Reissner
R2613	T5073	T5074	punct	(,Fig.
R2614	T5074	T5039	parataxis	Fig.,expressed
R2615	T5075	T5074	nummod	1c,Fig.
R2616	T5076	T5074	punct	),Fig.
R2617	T5077	T5037	punct	.,demonstrated
R2618	T5079	T5080	advmod	In,situ
R2619	T5080	T5081	amod	situ,hybridization
R262	T524	T522	amod	necessary,proteins
R2620	T5081	T5082	nsubj	hybridization,suggested
R2621	T5083	T5081	prep	in,hybridization
R2622	T5084	T5085	det	the,labyrinth
R2623	T5085	T5083	pobj	labyrinth,in
R2624	T5086	T5085	amod	vestibular,labyrinth
R2625	T5087	T5088	mark	that,expressed
R2626	T5088	T5082	ccomp	expressed,suggested
R2627	T5089	T5090	compound	pendrin,mRNA
R2628	T5090	T5088	nsubjpass	mRNA,expressed
R2629	T5091	T5088	auxpass	is,expressed
R263	T525	T524	prep	for,necessary
R2630	T5092	T5088	prep	in,expressed
R2631	T5093	T5094	amod	non-sensory,cells
R2632	T5094	T5092	pobj	cells,in
R2633	T5095	T5096	punct	[,8
R2634	T5096	T5082	parataxis	8,suggested
R2635	T5097	T5096	punct	],8
R2636	T5098	T5082	punct	.,suggested
R2637	T5100	T5101	advcl	Using,observed
R2638	T5102	T5103	amod	confocal,immunocytochemistry
R2639	T5103	T5100	dobj	immunocytochemistry,Using
R264	T526	T527	nmod	K,secretion
R2640	T5104	T5100	prep	on,Using
R2641	T5105	T5104	pobj	cryosections,on
R2642	T5106	T5101	punct	", ",observed
R2643	T5107	T5101	nsubj	we,observed
R2644	T5108	T5109	amod	strong,expression
R2645	T5109	T5101	dobj	expression,observed
R2646	T5110	T5109	prep	of,expression
R2647	T5111	T5112	det	the,protein
R2648	T5112	T5110	pobj	protein,of
R2649	T5113	T5112	compound	pendrin,protein
R265	T527	T525	pobj	secretion,for
R2650	T5114	T5101	prep	in,observed
R2651	T5115	T5116	det	the,membrane
R2652	T5116	T5114	pobj	membrane,in
R2653	T5117	T5116	amod	apical,membrane
R2654	T5118	T5116	prep	of,membrane
R2655	T5119	T5120	amod	vestibular,cells
R2656	T5120	T5118	pobj	cells,of
R2657	T5121	T5120	amod	transitional,cells
R2658	T5122	T5120	prep	in,cells
R2659	T5123	T5124	det	the,utricle
R266	T528	T526	punct	+,K
R2660	T5124	T5122	pobj	utricle,in
R2661	T5125	T5124	cc	and,utricle
R2662	T5126	T5124	conj	ampullae,utricle
R2663	T5127	T5128	punct	(,1i
R2664	T5128	T5101	parataxis	1i,observed
R2665	T5129	T5128	nmod	Fig.,1i
R2666	T5130	T5128	nummod	1g,1i
R2667	T5131	T5128	punct	",",1i
R2668	T5132	T5128	nummod	1h,1i
R2669	T5133	T5128	punct	",",1i
R267	T529	T523	acomp	present,were
R2670	T5140	T5138	prep	with,labeling
R2671	T5134	T5128	punct	),1i
R2672	T5141	T5140	pobj	KCNQ1,with
R2673	T5142	T5143	mark	that,limited
R2674	T5135	T5101	punct	.,observed
R2675	T5143	T5139	ccomp	limited,demonstrated
R2676	T5144	T5145	compound	pendrin,expression
R2677	T5145	T5143	nsubjpass	expression,limited
R2678	T5137	T5138	amod	Dual,labeling
R2679	T5146	T5143	auxpass	was,limited
R268	T530	T523	punct	", ",were
R2680	T5147	T5143	advmod	clearly,limited
R2681	T5148	T5143	prep	to,limited
R2682	T5138	T5139	nsubj	labeling,demonstrated
R2683	T5149	T5150	amod	vestibular,cells
R2684	T5150	T5148	pobj	cells,to
R2685	T5151	T5150	amod	transitional,cells
R2686	T5245	T5238	xcomp	develop,start
R2687	T5152	T5143	cc	and,limited
R2688	T5153	T5154	aux	did,extend
R2689	T5154	T5143	conj	extend,limited
R269	T531	T523	prep	including,were
R2690	T5155	T5154	neg	not,extend
R2691	T5156	T5154	prep	to,extend
R2692	T5246	T5247	det	an,space
R2693	T5157	T5158	amod	other,cells
R2694	T5158	T5156	pobj	cells,to
R2695	T5159	T5158	amod	non-sensory,cells
R2696	T5247	T5245	dobj	space,develop
R2697	T5160	T5161	amod	such,as
R2698	T5161	T5158	prep	as,cells
R2699	T5248	T5247	amod	enlarged,space
R27	T261	T260	amod	common,disorder
R270	T532	T533	det	the,channel
R2700	T5162	T5163	amod	vestibular,cells
R2701	T5163	T5161	pobj	cells,as
R2702	T5164	T5163	amod	dark,cells
R2703	T5249	T5247	amod	endolymphatic,space
R2704	T5165	T5163	punct	", ",cells
R2705	T5166	T5167	dep	which,identified
R2706	T5250	T5251	dep	that,persists
R2707	T5167	T5163	relcl	identified,cells
R2708	T5168	T5167	auxpass	were,identified
R2709	T5169	T5167	advmod	clearly,identified
R271	T533	T531	pobj	channel,including
R2710	T5251	T5247	relcl	persists,space
R2711	T5170	T5167	prep	by,identified
R2712	T5171	T5172	det	the,expression
R2713	T5172	T5170	pobj	expression,by
R2714	T5173	T5172	prep	of,expression
R2715	T5174	T5173	pobj	KCNQ1,of
R2716	T5175	T5174	cc	and,KCNQ1
R2717	T5252	T5251	prep	into,persists
R2718	T5176	T5174	conj	KCNE1,KCNQ1
R2719	T5177	T5172	prep	in,expression
R272	T534	T533	nmod	K,channel
R2720	T5178	T5179	poss	their,membranes
R2721	T5253	T5252	pobj	adulthood,into
R2722	T5179	T5177	pobj	membranes,in
R2723	T5180	T5179	amod	apical,membranes
R2724	T5181	T5139	punct	.,demonstrated
R2725	T5254	T5255	punct	[,14
R2726	T5183	T5184	det	The,onset
R2727	T5184	T5185	nsubjpass	onset,determined
R2728	T5255	T5245	parataxis	14,develop
R2729	T5186	T5184	prep	of,onset
R273	T535	T534	punct	+,K
R2730	T5256	T5255	punct	],14
R2731	T5187	T5188	compound	pendrin,expression
R2732	T5188	T5186	pobj	expression,of
R2733	T5189	T5184	prep	during,onset
R2734	T5257	T5214	punct	.,become
R2735	T5190	T5189	pobj	development,during
R2736	T5191	T5190	prep	of,development
R2737	T5192	T5193	det	the,ear
R2738	T5259	T5260	advmod	Interestingly,appear
R2739	T5261	T5260	punct	", ",appear
R274	T536	T533	appos	KCNQ1,channel
R2740	T5193	T5191	pobj	ear,of
R2741	T5194	T5193	nmod	mouse,ear
R2742	T5195	T5193	amod	inner,ear
R2743	T5262	T5263	amod	sensory,cells
R2744	T5196	T5185	aux	has,determined
R2745	T5197	T5185	auxpass	been,determined
R2746	T5198	T5199	aux	to,be
R2747	T5263	T5260	nsubj	cells,appear
R2748	T5199	T5185	xcomp	be,determined
R2749	T5200	T5201	amod	embryonic,day
R275	T537	T536	cc	and,KCNQ1
R2750	T5264	T5263	compound	hair,cells
R2751	T5201	T5199	attr	day,be
R2752	T5202	T5203	punct	(,ED
R2753	T5265	T5263	prep	in,cells
R2754	T5203	T5201	appos	ED,day
R2755	T5204	T5203	punct	),ED
R2756	T5205	T5201	nummod	13,day
R2757	T5266	T5267	det	the,cochlea
R2758	T5206	T5207	punct	[,14
R2759	T5207	T5185	parataxis	14,determined
R276	T538	T536	conj	KCNE1,KCNQ1
R2760	T5208	T5207	punct	],14
R2761	T5209	T5185	punct	.,determined
R2762	T5267	T5265	pobj	cochlea,in
R2763	T5211	T5212	advmod	Morphologically,detectable
R2764	T5212	T5213	amod	detectable,differences
R2765	T5213	T5214	nsubj	differences,become
R2766	T5268	T5260	oprd	normal,appear
R2767	T5215	T5213	prep	in,differences
R2768	T5216	T5217	det	the,ears
R2769	T5269	T5260	prep	until,appear
R277	T539	T533	punct	", ",channel
R2770	T5217	T5215	pobj	ears,in
R2771	T5218	T5217	amod	inner,ears
R2772	T5219	T5217	prep	of,ears
R2773	T5270	T5271	amod	postnatal,day
R2774	T5220	T5221	nmod	Slc26a4,mice
R2775	T5221	T5219	pobj	mice,of
R2776	T5222	T5220	punct	+,Slc26a4
R2777	T5271	T5269	pobj	day,until
R2778	T5223	T5220	punct	/,Slc26a4
R2779	T5224	T5220	punct	+,Slc26a4
R278	T540	T541	nmod	Na,cotransporter
R2780	T5225	T5220	cc	and,Slc26a4
R2781	T5272	T5273	punct	(,PD
R2782	T5226	T5220	conj	Slc26a4,Slc26a4
R2783	T5227	T5226	punct	-,Slc26a4
R2784	T5228	T5226	punct	/,Slc26a4
R2785	T5273	T5271	appos	PD,day
R2786	T5229	T5226	punct	-,Slc26a4
R2787	T5230	T5214	acomp	evident,become
R2788	T5231	T5232	advmod	as,early
R2789	T5274	T5273	punct	),PD
R279	T541	T549	compound	cotransporter,SLC12A2
R2790	T5232	T5214	advmod	early,become
R2791	T5233	T5232	prep	as,early
R2792	T5234	T5233	pobj	ED,as
R2793	T5275	T5271	nummod	7,day
R2794	T5235	T5234	nummod	15,ED
R2795	T5236	T5234	punct	", ",ED
R2796	T5276	T5260	cc	but,appear
R2797	T5237	T5238	advmod	when,start
R2798	T5238	T5234	advcl	start,ED
R2799	T5239	T5240	nmod	Slc26a4,mice
R28	T262	T263	amod	autosomal,recessive
R280	T542	T540	punct	+,Na
R2800	T5277	T5260	conj	show,appear
R2801	T5240	T5238	nsubj	mice,start
R2802	T5241	T5239	punct	-,Slc26a4
R2803	T5278	T5279	amod	clear,evidence
R2804	T5242	T5239	punct	/,Slc26a4
R2805	T5243	T5239	punct	-,Slc26a4
R2806	T5244	T5245	aux	to,develop
R2807	T5279	T5277	dobj	evidence,show
R2808	T5280	T5279	prep	of,evidence
R2809	T5281	T5280	pobj	degeneration,of
R281	T543	T540	punct	/,Na
R2810	T5282	T5277	prep	by,show
R2811	T5351	T5343	prep	until,persist
R2812	T5283	T5282	pobj	PD,by
R2813	T5352	T5351	pobj	PD,until
R2814	T5353	T5352	nummod	7,PD
R2815	T5354	T5321	punct	.,established
R2816	T5356	T5357	det	The,period
R2817	T5284	T5283	nummod	15,PD
R2818	T5357	T5359	nsubj	period,is
R2819	T5358	T5357	compound	time,period
R282	T544	T540	appos	2Cl,Na
R2820	T5360	T5357	prep	between,period
R2821	T5285	T5286	punct	[,14
R2822	T5361	T5362	nmod	PD,7
R2823	T5362	T5360	pobj	7,between
R2824	T5286	T5277	parataxis	14,show
R2825	T5363	T5362	cc	and,7
R2826	T5364	T5362	conj	15,7
R2827	T5287	T5286	punct	],14
R2828	T5365	T5366	det	the,time
R2829	T5288	T5260	punct	.,appear
R283	T545	T544	punct	-,2Cl
R2830	T5366	T5359	attr	time,is
R2831	T5367	T5368	advmod	when,develops
R2832	T5290	T5291	det	These,observations
R2833	T5368	T5366	relcl	develops,time
R2834	T5369	T5370	det	the,potential
R2835	T5370	T5368	nsubj	potential,develops
R2836	T5371	T5370	amod	endocochlear,potential
R2837	T5291	T5292	nsubj	observations,suggest
R2838	T5372	T5368	prep	at,develops
R2839	T5373	T5374	det	the,onset
R284	T546	T540	punct	/,Na
R2840	T5293	T5294	mark	that,supports
R2841	T5374	T5372	pobj	onset,at
R2842	T5375	T5374	prep	of,onset
R2843	T5376	T5375	pobj	hearing,of
R2844	T5294	T5292	ccomp	supports,suggest
R2845	T5377	T5378	punct	[,22
R2846	T5378	T5368	parataxis	22,develops
R2847	T5379	T5378	punct	],22
R2848	T5295	T5296	det	the,environment
R2849	T5296	T5294	nsubj	environment,supports
R285	T547	T540	appos	K,Na
R2850	T5380	T5359	punct	.,is
R2851	T5297	T5296	amod	cochlear,environment
R2852	T5382	T5383	nsubj	We,hypothesized
R2853	T5384	T5385	mark	that,account
R2854	T5298	T5299	det	the,survival
R2855	T5385	T5383	ccomp	account,hypothesized
R2856	T5386	T5387	det	a,lack
R2857	T5299	T5294	dobj	survival,supports
R2858	T5387	T5385	nsubj	lack,account
R2859	T5388	T5387	prep	of,lack
R286	T548	T547	punct	+,K
R2860	T5389	T5390	det	a,potential
R2861	T5390	T5388	pobj	potential,of
R2862	T5300	T5299	prep	of,survival
R2863	T5391	T5390	amod	normal,potential
R2864	T5392	T5390	amod	endocochlear,potential
R2865	T5393	T5387	cc	or,lack
R2866	T5394	T5395	det	an,alteration
R2867	T5395	T5387	conj	alteration,lack
R2868	T5396	T5395	prep	of,alteration
R2869	T5301	T5302	amod	sensory,cells
R287	T549	T533	conj	SLC12A2,channel
R2870	T5397	T5398	det	the,concentration
R2871	T5398	T5396	pobj	concentration,of
R2872	T5399	T5398	amod	endolymphatic,concentration
R2873	T5302	T5300	pobj	cells,of
R2874	T5400	T5398	nmod	K,concentration
R2875	T5401	T5400	punct	+,K
R2876	T5303	T5302	compound	hair,cells
R2877	T5402	T5385	aux	could,account
R2878	T5403	T5385	prep	for,account
R2879	T5404	T5403	pobj	deafness,for
R288	T550	T549	cc	and,SLC12A2
R2880	T5304	T5294	prep	in,supports
R2881	T5405	T5385	prep	in,account
R2882	T5406	T5407	nmod	Slc26a4,mice
R2883	T5305	T5304	pobj	spite,in
R2884	T5407	T5405	pobj	mice,in
R2885	T5408	T5406	punct	-,Slc26a4
R2886	T5409	T5406	punct	/,Slc26a4
R2887	T5306	T5305	prep	of,spite
R2888	T5410	T5406	punct	-,Slc26a4
R2889	T5411	T5383	punct	.,hypothesized
R289	T551	T552	det	the,junction
R2890	T5307	T5308	det	the,enlargement
R2891	T5413	T5414	nsubj	Measurements,revealed
R2892	T5308	T5306	pobj	enlargement,of
R2893	T5415	T5416	mark	that,was
R2894	T5416	T5414	advcl	was,revealed
R2895	T5417	T5418	det	the,potential
R2896	T5309	T5308	prep	of,enlargement
R2897	T5418	T5416	nsubj	potential,was
R2898	T5419	T5418	amod	endocochlear,potential
R2899	T5420	T5416	acomp	absent,was
R29	T263	T260	amod	recessive,disorder
R290	T552	T549	conj	junction,SLC12A2
R2900	T5310	T5311	det	the,duct
R2901	T5421	T5416	cc	but,was
R2902	T5422	T5423	mark	that,was
R2903	T5311	T5309	pobj	duct,of
R2904	T5423	T5416	conj	was,was
R2905	T5424	T5425	det	the,concentration
R2906	T5425	T5423	nsubj	concentration,was
R2907	T5312	T5311	amod	endolymphatic,duct
R2908	T5426	T5425	amod	endolymphatic,concentration
R2909	T5427	T5425	nmod	K,concentration
R291	T553	T552	compound	gap,junction
R2910	T5428	T5427	punct	+,K
R2911	T5313	T5292	punct	.,suggest
R2912	T5429	T5423	acomp	normal,was
R2913	T5430	T5423	prep	in,was
R2914	T5315	T5316	det	A,concentration
R2915	T5431	T5432	amod	adult,Slc26a4
R2916	T5432	T5430	pobj	Slc26a4,in
R2917	T5433	T5432	punct	-,Slc26a4
R2918	T5434	T5432	punct	/,Slc26a4
R2919	T5435	T5432	punct	-,Slc26a4
R292	T554	T552	appos	GJB2,junction
R2920	T5436	T5437	punct	(,Fig.
R2921	T5316	T5321	nsubjpass	concentration,established
R2922	T5437	T5423	parataxis	Fig.,was
R2923	T5317	T5316	amod	normal,concentration
R2924	T5438	T5437	nummod	2a,Fig.
R2925	T5318	T5316	amod	endolymphatic,concentration
R2926	T5439	T5437	punct	),Fig.
R2927	T5319	T5316	nmod	K,concentration
R2928	T5440	T5414	punct	.,revealed
R2929	T5442	T5443	det	No,differences
R293	T555	T523	punct	.,were
R2930	T5320	T5319	punct	+,K
R2931	T5443	T5445	nsubjpass	differences,found
R2932	T5322	T5316	punct	", ",concentration
R2933	T5444	T5443	amod	significant,differences
R2934	T5446	T5443	prep	between,differences
R2935	T5447	T5448	nmod	Slc26a4,mice
R2936	T5323	T5324	dep	which,is
R2937	T5324	T5316	relcl	is,concentration
R2938	T5325	T5324	acomp	critical,is
R2939	T5326	T5325	prep	for,critical
R294	T559	T560	det	These,observations
R2940	T5448	T5446	pobj	mice,between
R2941	T5327	T5328	compound	hair,cell
R2942	T5449	T5447	punct	+,Slc26a4
R2943	T5450	T5447	punct	/,Slc26a4
R2944	T5328	T5329	compound	cell,survival
R2945	T5451	T5447	punct	+,Slc26a4
R2946	T5452	T5447	cc	and,Slc26a4
R2947	T5453	T5447	conj	Slc26a4,Slc26a4
R2948	T5329	T5326	pobj	survival,for
R2949	T5454	T5453	punct	-,Slc26a4
R295	T560	T561	nsubj	observations,demonstrate
R2950	T5455	T5453	punct	/,Slc26a4
R2951	T5456	T5453	punct	-,Slc26a4
R2952	T5330	T5331	punct	[,20
R2953	T5331	T5324	parataxis	20,is
R2954	T5457	T5445	auxpass	were,found
R2955	T5332	T5331	punct	],20
R2956	T5458	T5445	prep	in,found
R2957	T5459	T5460	amod	perilymphatic,concentrations
R2958	T5333	T5321	punct	", ",established
R2959	T5460	T5458	pobj	concentrations,in
R296	T562	T563	mark	that,leads
R2960	T5461	T5462	punct	(,Fig.
R2961	T5334	T5321	auxpass	is,established
R2962	T5462	T5459	parataxis	Fig.,perilymphatic
R2963	T5463	T5462	nummod	2a,Fig.
R2964	T5464	T5462	punct	),Fig.
R2965	T5335	T5321	prep	at,established
R2966	T5465	T5459	cc	or,perilymphatic
R2967	T5466	T5467	compound	plasma,K
R2968	T5467	T5459	conj	K,perilymphatic
R2969	T5336	T5335	pobj	PD,at
R297	T563	T561	ccomp	leads,demonstrate
R2970	T5468	T5467	punct	+,K
R2971	T5469	T5470	punct	(,6
R2972	T5470	T5445	parataxis	6,found
R2973	T5471	T5472	dep	Slc26a4,6
R2974	T5472	T5470	dep	6,6
R2975	T5473	T5471	punct	+,Slc26a4
R2976	T5474	T5471	punct	/,Slc26a4
R2977	T5337	T5336	nummod	3,PD
R2978	T5475	T5471	punct	+,Slc26a4
R2979	T5476	T5472	punct	", ",6
R298	T564	T565	compound	pendrin,dysfunction
R2980	T5477	T5478	quantmod	4.9,0.3
R2981	T5338	T5339	punct	[,21
R2982	T5478	T5480	nummod	0.3,mM
R2983	T5479	T5478	punct	±,0.3
R2984	T5480	T5472	dep	mM,6
R2985	T5339	T5321	parataxis	21,established
R2986	T5481	T5472	punct	", ",6
R2987	T5482	T5472	nsubj	n,6
R2988	T5483	T5472	punct	=,6
R2989	T5340	T5339	punct	],21
R299	T565	T563	nsubj	dysfunction,leads
R2990	T5484	T5470	punct	;,6
R2991	T5485	T5470	dep	Slc26a4,6
R2992	T5341	T5321	cc	and,established
R2993	T5486	T5485	punct	-,Slc26a4
R2994	T5487	T5485	punct	/,Slc26a4
R2995	T5488	T5485	punct	-,Slc26a4
R2996	T5342	T5343	aux	may,persist
R2997	T5489	T5470	punct	", ",6
R2998	T5490	T5491	quantmod	5.1,0.3
R2999	T5491	T5493	nummod	0.3,mM
R30	T264	T263	punct	-,recessive
R300	T566	T563	prep	to,leads
R3000	T5343	T5321	conj	persist,established
R3001	T5492	T5491	punct	±,0.3
R3002	T5493	T5470	dep	mM,6
R3003	T5494	T5470	punct	", ",6
R3004	T5344	T5343	prep	in,persist
R3005	T5495	T5470	nsubj	n,6
R3006	T5496	T5470	punct	=,6
R3007	T5497	T5470	punct	),6
R3008	T5345	T5344	pobj	Slc26a4,in
R3009	T5498	T5445	punct	.,found
R301	T567	T568	det	a,loss
R3010	T5500	T5501	det	These,observations
R3011	T5346	T5345	punct	-,Slc26a4
R3012	T5501	T5502	nsubj	observations,suggest
R3013	T5347	T5345	punct	/,Slc26a4
R3014	T5503	T5504	mark	that,is
R3015	T5504	T5502	ccomp	is,suggest
R3016	T5505	T5506	det	a,event
R3017	T5348	T5345	punct	-,Slc26a4
R3018	T5506	T5504	nsubj	event,is
R3019	T5507	T5506	amod	primary,event
R302	T568	T566	pobj	loss,to
R3020	T5508	T5506	acl	leading,event
R3021	T5349	T5350	advmod	at,least
R3022	T5509	T5508	prep	to,leading
R3023	T5510	T5509	pobj	deafness,to
R3024	T5511	T5508	prep	in,leading
R3025	T5512	T5513	nmod	Slc26a4,mice
R3026	T5513	T5511	pobj	mice,in
R3027	T5514	T5512	punct	-,Slc26a4
R3028	T5350	T5351	advmod	least,until
R3029	T5515	T5512	punct	/,Slc26a4
R303	T569	T568	prep	of,loss
R3030	T5516	T5512	punct	-,Slc26a4
R3031	T5517	T5511	punct	", ",in
R3032	T5553	T5554	det	The,potential
R3033	T5518	T5511	cc	and,in
R3034	T5519	T5520	advmod	potentially,in
R3035	T5520	T5511	conj	in,in
R3036	T5521	T5520	pobj	patients,in
R3037	T5522	T5521	acl	suffering,patients
R3038	T5523	T5522	prep	from,suffering
R3039	T5524	T5525	compound	Pendred,syndrome
R304	T570	T571	compound	KCNJ10,expression
R3040	T5525	T5523	pobj	syndrome,from
R3041	T5554	T5556	nsubjpass	potential,generated
R3042	T5526	T5504	punct	", ",is
R3043	T5527	T5528	det	the,loss
R3044	T5528	T5504	attr	loss,is
R3045	T5529	T5528	prep	of,loss
R3046	T5555	T5554	amod	endocochlear,potential
R3047	T5530	T5531	det	the,potential
R3048	T5531	T5529	pobj	potential,of
R3049	T5532	T5531	amod	endocochlear,potential
R305	T571	T569	pobj	expression,of
R3050	T5557	T5556	auxpass	is,generated
R3051	T5533	T5502	punct	.,suggest
R3052	T5535	T5536	nsubj	Degeneration,is
R3053	T5558	T5556	agent	by,generated
R3054	T5559	T5560	compound	stria,vascularis
R3055	T5537	T5535	prep	of,Degeneration
R3056	T5538	T5539	compound	hair,cells
R3057	T5560	T5558	pobj	vascularis,by
R3058	T5539	T5537	pobj	cells,of
R3059	T5540	T5536	advmod	probably,is
R306	T572	T571	compound	protein,expression
R3060	T5541	T5542	det	a,response
R3061	T5561	T5556	prep	in,generated
R3062	T5542	T5536	attr	response,is
R3063	T5543	T5542	prep	to,response
R3064	T5544	T5545	det	the,loss
R3065	T5562	T5563	det	the,wall
R3066	T5545	T5543	pobj	loss,to
R3067	T5546	T5545	prep	of,loss
R3068	T5563	T5561	pobj	wall,in
R3069	T5547	T5548	det	the,potential
R307	T573	T568	cc	and,loss
R3070	T5548	T5546	pobj	potential,of
R3071	T5549	T5548	amod	endocochlear,potential
R3072	T5550	T5536	punct	.,is
R3073	T5564	T5563	amod	lateral,wall
R3074	T5565	T5563	prep	of,wall
R3075	T5566	T5567	det	the,cochlea
R3076	T5619	T5620	amod	Marginal,cells
R3077	T5620	T5621	nsubj	cells,transport
R3078	T5567	T5565	pobj	cochlea,of
R3079	T5622	T5620	prep	of,cells
R308	T574	T575	det	a,loss
R3080	T5623	T5624	compound	stria,vascularis
R3081	T5568	T5569	punct	[,23
R3082	T5624	T5622	pobj	vascularis,of
R3083	T5625	T5620	punct	", ",cells
R3084	T5626	T5627	dep	which,form
R3085	T5569	T5556	parataxis	23,generated
R3086	T5627	T5620	relcl	form,cells
R3087	T5628	T5629	det	the,barrier
R3088	T5629	T5627	dobj	barrier,form
R3089	T5570	T5569	nummod	17,23
R309	T575	T568	conj	loss,loss
R3090	T5630	T5627	prep	toward,form
R3091	T5631	T5630	pobj	endolymph,toward
R3092	T5632	T5621	punct	", ",transport
R3093	T5571	T5569	punct	",",23
R3094	T5633	T5621	dobj	K,transport
R3095	T5634	T5633	punct	+,K
R3096	T5572	T5569	punct	],23
R3097	T5635	T5621	prep	from,transport
R3098	T5636	T5637	det	the,space
R3099	T5637	T5635	pobj	space,from
R31	T265	T260	acl	characterized,disorder
R310	T576	T575	prep	of,loss
R3100	T5573	T5556	punct	.,generated
R3101	T5638	T5637	amod	intrastrial,space
R3102	T5639	T5621	prep	into,transport
R3103	T5575	T5576	det	The,potential
R3104	T5640	T5639	pobj	endolymph,into
R3105	T5641	T5621	cc	and,transport
R3106	T5642	T5621	conj	keep,transport
R3107	T5643	T5644	det	the,concentration
R3108	T5576	T5577	nsubjpass	potential,generated
R3109	T5644	T5642	dobj	concentration,keep
R311	T577	T578	det	the,potential
R3110	T5645	T5644	nmod	K,concentration
R3111	T5646	T5645	punct	+,K
R3112	T5578	T5577	auxpass	is,generated
R3113	T5647	T5642	oprd	low,keep
R3114	T5579	T5577	prep	across,generated
R3115	T5648	T5642	advcl	adjacent,keep
R3116	T5649	T5648	prep	to,adjacent
R3117	T5580	T5581	det	the,barrier
R3118	T5650	T5651	det	the,channel
R3119	T5651	T5649	pobj	channel,to
R312	T578	T576	pobj	potential,of
R3120	T5652	T5651	nmod	KCNJ10,channel
R3121	T5581	T5579	pobj	barrier,across
R3122	T5653	T5651	nmod	K,channel
R3123	T5654	T5653	punct	+,K
R3124	T5655	T5656	punct	[,26
R3125	T5582	T5583	amod	basal,cell
R3126	T5656	T5642	parataxis	26,keep
R3127	T5657	T5656	punct	],26
R3128	T5658	T5621	punct	.,transport
R3129	T5660	T5661	aux	To,determine
R313	T579	T578	amod	endocochlear,potential
R3130	T5583	T5581	compound	cell,barrier
R3131	T5661	T5662	advcl	determine,determined
R3132	T5584	T5581	prep	of,barrier
R3133	T5663	T5664	det	the,cause
R3134	T5664	T5661	dobj	cause,determine
R3135	T5665	T5664	prep	of,cause
R3136	T5585	T5586	compound	stria,vascularis
R3137	T5666	T5667	det	the,loss
R3138	T5667	T5665	pobj	loss,of
R3139	T5586	T5584	pobj	vascularis,of
R314	T580	T575	punct	", ",loss
R3140	T5668	T5667	prep	of,loss
R3141	T5669	T5670	det	the,potential
R3142	T5670	T5668	pobj	potential,of
R3143	T5587	T5577	agent	by,generated
R3144	T5671	T5670	amod	endocochlear,potential
R3145	T5672	T5667	prep	in,loss
R3146	T5673	T5674	nmod	Slc26a4,mice
R3147	T5588	T5589	det	the,KCNJ10
R3148	T5674	T5672	pobj	mice,in
R3149	T5675	T5673	punct	-,Slc26a4
R315	T581	T582	dep	which,be
R3150	T5676	T5673	punct	/,Slc26a4
R3151	T5589	T5587	pobj	KCNJ10,by
R3152	T5677	T5673	punct	-,Slc26a4
R3153	T5678	T5662	punct	", ",determined
R3154	T5590	T5589	nmod	K,KCNJ10
R3155	T5679	T5662	nsubj	we,determined
R3156	T5591	T5590	punct	+,K
R3157	T5680	T5662	advmod	first,determined
R3158	T5592	T5589	compound	channel,KCNJ10
R3159	T5681	T5682	mark	whether,are
R316	T582	T575	relcl	be,loss
R3160	T5682	T5662	ccomp	are,determined
R3161	T5683	T5684	amod	intermediate,cells
R3162	T5593	T5589	acl	located,KCNJ10
R3163	T5684	T5682	nsubj	cells,are
R3164	T5685	T5682	acomp	present,are
R3165	T5594	T5593	prep	in,located
R3166	T5686	T5682	prep	in,are
R3167	T5687	T5688	compound	stria,vascularis
R3168	T5688	T5686	pobj	vascularis,in
R3169	T5595	T5596	amod	intermediate,cells
R317	T583	T582	aux	may,be
R3170	T5689	T5662	punct	", ",determined
R3171	T5690	T5691	mark	since,known
R3172	T5691	T5662	advcl	known,determined
R3173	T5596	T5594	pobj	cells,in
R3174	T5692	T5693	det	a,loss
R3175	T5693	T5691	nsubjpass	loss,known
R3176	T5694	T5693	prep	of,loss
R3177	T5597	T5598	punct	[,24
R3178	T5695	T5696	amod	intermediate,cells
R3179	T5696	T5694	pobj	cells,of
R318	T584	T585	det	the,cause
R3180	T5697	T5691	auxpass	is,known
R3181	T5698	T5699	aux	to,lead
R3182	T5598	T5596	parataxis	24,cells
R3183	T5699	T5691	xcomp	lead,known
R3184	T5700	T5699	prep	to,lead
R3185	T5599	T5598	punct	],24
R3186	T5701	T5702	det	a,loss
R3187	T5702	T5700	pobj	loss,to
R3188	T5600	T5596	punct	", ",cells
R3189	T5703	T5702	prep	of,loss
R319	T585	T582	attr	cause,be
R3190	T5704	T5705	det	the,potential
R3191	T5601	T5602	dep	which,connected
R3192	T5705	T5703	pobj	potential,of
R3193	T5602	T5596	relcl	connected,cells
R3194	T5706	T5705	amod	endocochlear,potential
R3195	T5707	T5708	punct	[,28
R3196	T5603	T5602	auxpass	are,connected
R3197	T5708	T5691	parataxis	28,known
R3198	T5709	T5708	nummod	27,28
R3199	T5710	T5708	punct	",",28
R32	T266	T265	agent	by,characterized
R320	T586	T585	amod	direct,cause
R3200	T5604	T5602	prep	to,connected
R3201	T5711	T5708	punct	],28
R3202	T5712	T5662	punct	.,determined
R3203	T5605	T5606	amod	basal,cells
R3204	T5714	T5715	amod	Intermediate,cells
R3205	T5715	T5716	nsubjpass	cells,visualized
R3206	T5606	T5604	pobj	cells,to
R3207	T5717	T5715	prep	of,cells
R3208	T5718	T5719	compound	stria,vascularis
R3209	T5607	T5602	prep	by,connected
R321	T587	T585	prep	of,cause
R3210	T5719	T5717	pobj	vascularis,of
R3211	T5720	T5716	auxpass	were,visualized
R3212	T5608	T5609	det	a,density
R3213	T5721	T5716	prep	by,visualized
R3214	T5722	T5723	poss	their,pigmentation
R3215	T5723	T5721	pobj	pigmentation,by
R3216	T5609	T5607	pobj	density,by
R3217	T5724	T5716	punct	.,visualized
R3218	T5610	T5609	amod	high,density
R3219	T5611	T5609	prep	of,density
R322	T588	T587	pobj	deafness,of
R3220	T5726	T5727	nsubj	Pigmentation,was
R3221	T5612	T5613	compound	gap,junctions
R3222	T5728	T5727	acomp	present,was
R3223	T5729	T5727	prep	in,was
R3224	T5613	T5611	pobj	junctions,of
R3225	T5730	T5731	compound	stria,vascularis
R3226	T5731	T5729	pobj	vascularis,in
R3227	T5732	T5731	prep	of,vascularis
R3228	T5614	T5615	punct	[,25
R3229	T5733	T5734	nmod	Slc26a4,mice
R323	T589	T585	prep	in,cause
R3230	T5615	T5602	parataxis	25,connected
R3231	T5734	T5732	pobj	mice,of
R3232	T5735	T5733	punct	-,Slc26a4
R3233	T5736	T5733	punct	/,Slc26a4
R3234	T5737	T5733	punct	-,Slc26a4
R3235	T5738	T5739	punct	(,Fig.
R3236	T5616	T5615	punct	],25
R3237	T5739	T5727	parataxis	Fig.,was
R3238	T5740	T5739	nummod	2b,Fig.
R3239	T5741	T5739	punct	),Fig.
R324	T590	T591	compound	Pendred,syndrome
R3240	T5617	T5577	punct	.,generated
R3241	T5742	T5727	punct	", ",was
R3242	T5743	T5744	dep	which,suggests
R3243	T5831	T5820	punct	/,Slc26a4
R3244	T5744	T5727	advcl	suggests,was
R3245	T5745	T5746	mark	that,are
R3246	T5746	T5744	ccomp	are,suggests
R3247	T5747	T5748	amod	intermediate,cells
R3248	T5748	T5746	nsubj	cells,are
R3249	T5749	T5746	acomp	present,are
R325	T591	T589	pobj	syndrome,in
R3250	T5750	T5727	punct	.,was
R3251	T5752	T5753	advmod	Interestingly,was
R3252	T5754	T5753	punct	", ",was
R3253	T5755	T5753	nsubj	pigmentation,was
R3254	T5832	T5820	punct	-,Slc26a4
R3255	T5756	T5755	prep	of,pigmentation
R3256	T5757	T5758	compound	stria,vascularis
R3257	T5758	T5756	pobj	vascularis,of
R3258	T5833	T5777	punct	", ",isolated
R3259	T5759	T5760	advmod	much,stronger
R326	T592	T561	punct	.,demonstrate
R3260	T5760	T5753	acomp	stronger,was
R3261	T5761	T5753	prep	in,was
R3262	T5834	T5777	conj	assessed,isolated
R3263	T5762	T5761	pobj	Slc26a4,in
R3264	T5763	T5762	punct	-,Slc26a4
R3265	T5835	T5834	dobj	amounts,assessed
R3266	T5764	T5762	punct	/,Slc26a4
R3267	T5765	T5762	punct	-,Slc26a4
R3268	T5766	T5753	prep	than,was
R3269	T5767	T5766	prep	in,than
R3270	T5836	T5835	prep	of,amounts
R3271	T5768	T5769	nmod	Slc26a4,mice
R3272	T5769	T5767	pobj	mice,in
R3273	T5770	T5768	punct	+,Slc26a4
R3274	T5837	T5838	amod	isolated,tissues
R3275	T5771	T5768	punct	/,Slc26a4
R3276	T5772	T5768	punct	+,Slc26a4
R3277	T5773	T5753	punct	.,was
R3278	T5838	T5836	pobj	tissues,of
R3279	T5775	T5776	aux	To,determine
R3280	T5776	T5777	advcl	determine,isolated
R3281	T5839	T5834	prep	by,assessed
R3282	T5778	T5776	prep	in,determine
R3283	T5779	T5780	amod	greater,detail
R3284	T5780	T5778	pobj	detail,in
R3285	T5781	T5782	det	the,cause
R3286	T5782	T5776	dobj	cause,determine
R3287	T5840	T5839	pobj	quantification,by
R3288	T5783	T5782	prep	of,cause
R3289	T5784	T5785	det	the,loss
R3290	T5785	T5783	pobj	loss,of
R3291	T5841	T5840	prep	of,quantification
R3292	T5786	T5785	prep	of,loss
R3293	T5787	T5788	det	the,potential
R3294	T5788	T5786	pobj	potential,of
R3295	T5842	T5843	compound	18S,rRNA
R3296	T5789	T5788	amod	endocochlear,potential
R3297	T5790	T5782	prep	in,cause
R3298	T5791	T5792	nmod	Slc26a4,mice
R3299	T5843	T5841	pobj	rRNA,of
R33	T267	T268	amod	congenital,deafness
R3300	T5792	T5790	pobj	mice,in
R3301	T5793	T5791	punct	-,Slc26a4
R3302	T5794	T5791	punct	/,Slc26a4
R3303	T5844	T5834	punct	", ",assessed
R3304	T5795	T5791	punct	-,Slc26a4
R3305	T5796	T5777	punct	", ",isolated
R3306	T5845	T5834	cc	and,assessed
R3307	T5797	T5777	nsubj	we,isolated
R3308	T5798	T5799	amod	total,RNA
R3309	T5799	T5777	dobj	RNA,isolated
R3310	T5846	T5834	conj	quantified,assessed
R3311	T5800	T5777	prep	from,isolated
R3312	T5801	T5802	compound	stria,vascularis
R3313	T5802	T5800	pobj	vascularis,from
R3314	T5847	T5848	det	the,expression
R3315	T5803	T5802	cc	and,vascularis
R3316	T5804	T5805	amod	spiral,ganglia
R3317	T5848	T5846	dobj	expression,quantified
R3318	T5805	T5802	conj	ganglia,vascularis
R3319	T5806	T5802	prep	of,vascularis
R332	T770	T771	compound	Pendred,syndrome
R3320	T5807	T5808	amod	young,Slc26a4
R3321	T5849	T5848	prep	of,expression
R3322	T5808	T5806	pobj	Slc26a4,of
R3323	T5809	T5810	punct	(,month
R3324	T5810	T5808	parataxis	month,Slc26a4
R3325	T5850	T5851	compound	KCNJ10,mRNA
R3326	T5811	T5812	quantmod	1,4
R3327	T5812	T5810	nummod	4,month
R3328	T5813	T5812	punct	–,4
R3329	T5851	T5849	pobj	mRNA,of
R333	T771	T772	nsubj	syndrome,is
R3330	T5814	T5810	punct	),month
R3331	T5815	T5808	punct	+,Slc26a4
R3332	T5816	T5808	punct	/,Slc26a4
R3333	T5852	T5777	punct	.,isolated
R3334	T5817	T5808	punct	+,Slc26a4
R3335	T5818	T5808	cc	and,Slc26a4
R3336	T5819	T5820	preconj	both,Slc26a4
R3337	T5854	T5855	nsubj	Quantities,were
R3338	T5820	T5829	nmod	Slc26a4,mice
R3339	T5821	T5820	amod	young,Slc26a4
R334	T773	T774	det	a,disorder
R3340	T5822	T5820	cc	and,Slc26a4
R3341	T5823	T5820	amod	old,Slc26a4
R3342	T5856	T5854	prep	of,Quantities
R3343	T5824	T5825	punct	(,month
R3344	T5825	T5820	parataxis	month,Slc26a4
R3345	T5826	T5827	punct	~,12
R3346	T5827	T5825	nummod	12,month
R3347	T5857	T5858	compound	stria,vascularis
R3348	T5828	T5825	punct	),month
R3349	T5829	T5808	conj	mice,Slc26a4
R335	T774	T772	attr	disorder,is
R3350	T5830	T5820	punct	-,Slc26a4
R3351	T5858	T5856	pobj	vascularis,of
R3352	T5859	T5854	acl	isolated,Quantities
R3353	T5860	T5859	prep	from,isolated
R3354	T5937	T5917	punct	),0.18
R3355	T5861	T5862	det	these,mice
R3356	T5938	T5939	advmod	significantly,smaller
R3357	T5862	T5860	pobj	mice,from
R3358	T5939	T5899	acomp	smaller,were
R3359	T5940	T5939	prep	than,smaller
R336	T775	T776	advmod	relatively,common
R3360	T5941	T5940	prep	in,than
R3361	T5863	T5862	amod	different,mice
R3362	T5942	T5943	nmod	Slc26a4,mice
R3363	T5943	T5941	pobj	mice,in
R3364	T5864	T5855	acomp	similar,were
R3365	T5944	T5942	punct	+,Slc26a4
R3366	T5945	T5942	punct	/,Slc26a4
R3367	T5865	T5855	punct	", ",were
R3368	T5946	T5942	punct	+,Slc26a4
R3369	T5947	T5948	punct	(,7
R337	T776	T774	amod	common,disorder
R3370	T5866	T5867	mark	since,found
R3371	T5948	T5943	parataxis	7,mice
R3372	T5949	T5950	nmod	log,rRNA
R3373	T5950	T5952	nsubj	rRNA,0.19
R3374	T5867	T5855	advcl	found,were
R3375	T5951	T5950	punct	(,rRNA
R3376	T5952	T5948	ccomp	0.19,7
R3377	T5868	T5869	det	no,differences
R3378	T5953	T5950	punct	),rRNA
R3379	T5954	T5952	punct	=,0.19
R338	T777	T778	amod	autosomal,recessive
R3380	T5955	T5952	nummod	9.48,0.19
R3381	T5869	T5867	nsubjpass	differences,found
R3382	T5956	T5952	punct	±,0.19
R3383	T5957	T5948	punct	", ",7
R3384	T5958	T5948	nsubj	n,7
R3385	T5870	T5869	amod	significant,differences
R3386	T5959	T5948	punct	=,7
R3387	T5960	T5948	punct	),7
R3388	T5961	T5899	punct	", ",were
R3389	T5962	T5899	advcl	consistent,were
R339	T778	T774	amod	recessive,disorder
R3390	T5963	T5962	prep	with,consistent
R3391	T5964	T5965	amod	morphometric,data
R3392	T5871	T5867	auxpass	were,found
R3393	T5965	T5963	pobj	data,with
R3394	T5966	T5967	punct	(,see
R3395	T5967	T5899	parataxis	see,were
R3396	T5872	T5867	prep	in,found
R3397	T5968	T5967	advmod	below,see
R3398	T5969	T5967	punct	),see
R3399	T5970	T5899	punct	.,were
R34	T268	T266	pobj	deafness,by
R340	T779	T778	punct	-,recessive
R3400	T5873	T5874	det	the,numbers
R3401	T5972	T5973	nsubj	Expression,was
R3402	T5874	T5872	pobj	numbers,in
R3403	T5974	T5972	prep	of,Expression
R3404	T5975	T5976	compound	KCNJ10,mRNA
R3405	T5976	T5974	pobj	mRNA,of
R3406	T5875	T5874	prep	of,numbers
R3407	T5977	T5973	acomp	normal,was
R3408	T5978	T5977	prep	in,normal
R3409	T5979	T5980	compound	stria,vascularis
R341	T780	T774	acl	characterized,disorder
R3410	T5876	T5877	compound	18S,molecules
R3411	T5980	T5978	pobj	vascularis,in
R3412	T5981	T5980	cc	and,vascularis
R3413	T5982	T5983	amod	spiral,ganglia
R3414	T5877	T5875	pobj	molecules,of
R3415	T5983	T5980	conj	ganglia,vascularis
R3416	T5984	T5980	prep	of,vascularis
R3417	T5985	T5986	amod	young,mice
R3418	T5878	T5877	compound	rRNA,molecules
R3419	T5879	T5880	punct	(,data
R342	T781	T780	agent	by,characterized
R3420	T5986	T5984	pobj	mice,of
R3421	T5987	T5986	nmod	Slc26a4,mice
R3422	T5880	T5867	parataxis	data,found
R3423	T5988	T5987	punct	-,Slc26a4
R3424	T5989	T5987	punct	/,Slc26a4
R3425	T5881	T5882	nmod	log,rRNA
R3426	T5990	T5987	punct	-,Slc26a4
R3427	T5991	T5977	cc	but,normal
R3428	T5992	T5993	advmod	significantly,reduced
R3429	T5993	T5977	conj	reduced,normal
R343	T782	T781	pobj	deafness,by
R3430	T5882	T5884	nsubj	rRNA,0.08
R3431	T5994	T5993	prep	in,reduced
R3432	T5995	T5996	amod	old,mice
R3433	T5883	T5882	punct	(,rRNA
R3434	T5996	T5994	pobj	mice,in
R3435	T5997	T5996	nmod	Slc26a4,mice
R3436	T5998	T5997	punct	-,Slc26a4
R3437	T5999	T5997	punct	/,Slc26a4
R3438	T5884	T5880	ccomp	0.08,data
R3439	T6000	T5997	punct	-,Slc26a4
R344	T783	T782	cc	and,deafness
R3440	T6001	T6002	punct	(,Fig.
R3441	T6002	T5993	parataxis	Fig.,reduced
R3442	T6003	T6002	nummod	3,Fig.
R3443	T6004	T6002	punct	),Fig.
R3444	T6005	T5973	punct	.,was
R3445	T5885	T5882	punct	),rRNA
R3446	T6007	T6008	nsubjpass	Quantifications,used
R3447	T5886	T5884	punct	=,0.08
R3448	T6009	T6007	prep	of,Quantifications
R3449	T6010	T6011	nmod	KCNQ1,mRNA
R345	T784	T782	conj	goiter,deafness
R3450	T6011	T6009	pobj	mRNA,of
R3451	T5887	T5884	nummod	9.46,0.08
R3452	T6012	T6010	cc	and,KCNQ1
R3453	T6013	T6010	conj	KCNQ4,KCNQ1
R3454	T6014	T6008	auxpass	were,used
R3455	T5888	T5884	punct	±,0.08
R3456	T6015	T6016	aux	to,assess
R3457	T6016	T6008	advcl	assess,used
R3458	T5889	T5880	punct	", ",data
R3459	T6017	T6018	amod	possible,cross-contamination
R346	T785	T786	punct	[,1
R3460	T6018	T6016	dobj	cross-contamination,assess
R3461	T6019	T6018	prep	between,cross-contamination
R3462	T5890	T5891	nsubj	n,17
R3463	T6020	T6021	det	the,preparations
R3464	T6021	T6019	pobj	preparations,between
R3465	T6022	T6023	nmod	stria,vascularis
R3466	T5891	T5880	ccomp	17,data
R3467	T6023	T6021	nmod	vascularis,preparations
R3468	T6024	T6023	cc	and,vascularis
R3469	T6025	T6026	amod	spiral,ganglia
R347	T786	T780	parataxis	1,characterized
R3470	T5892	T5891	punct	=,17
R3471	T6026	T6023	conj	ganglia,vascularis
R3472	T6027	T6008	prep	based,used
R3473	T6028	T6027	prep	on,based
R3474	T5893	T5880	punct	", ",data
R3475	T6029	T6030	det	the,assumptions
R3476	T6030	T6028	pobj	assumptions,on
R3477	T5894	T5880	amod	pooled,data
R3478	T6031	T6032	mark	that,expressed
R3479	T6032	T6030	acl	expressed,assumptions
R348	T787	T786	punct	],1
R3480	T6033	T6034	punct	(,1
R3481	T5895	T5880	punct	),data
R3482	T6034	T6032	meta	1,expressed
R3483	T6035	T6034	punct	),1
R3484	T6036	T6032	nsubjpass	KCNQ1,expressed
R3485	T6037	T6032	auxpass	is,expressed
R3486	T5896	T5855	punct	.,were
R3487	T6038	T6032	prep	in,expressed
R3488	T5898	T5899	prep	In,were
R3489	T6039	T6038	pobj	cells,in
R349	T788	T772	punct	.,is
R3490	T6040	T6039	prep	of,cells
R3491	T6041	T6042	det	the,vascularis
R3492	T5900	T5898	pobj	contrast,In
R3493	T5901	T5899	punct	", ",were
R3494	T6042	T6040	pobj	vascularis,of
R3495	T6043	T6042	compound	stria,vascularis
R3496	T5902	T5899	nsubj	quantities,were
R3497	T6044	T6042	cc	but,vascularis
R3498	T6045	T6044	neg	not,but
R3499	T5903	T5902	prep	of,quantities
R35	T269	T268	cc	and,deafness
R350	T790	T791	det	The,syndrome
R3500	T6046	T6047	det	the,preparation
R3501	T6047	T6042	conj	preparation,vascularis
R3502	T6048	T6047	amod	spiral,preparation
R3503	T5904	T5905	amod	spiral,ganglia
R3504	T6049	T6047	compound	ganglia,preparation
R3505	T6050	T6032	cc	and,expressed
R3506	T6051	T6052	punct	(,2
R3507	T5905	T5903	pobj	ganglia,of
R3508	T6052	T6053	meta	2,expressed
R3509	T6053	T6032	conj	expressed,expressed
R351	T791	T792	nsubjpass	syndrome,caused
R3510	T6054	T6052	punct	),2
R3511	T6055	T6053	nsubjpass	KCNQ4,expressed
R3512	T5906	T5902	acl	isolated,quantities
R3513	T6056	T6053	auxpass	is,expressed
R3514	T6057	T6053	prep	in,expressed
R3515	T5907	T5906	prep	from,isolated
R3516	T6058	T6057	pobj	cells,in
R3517	T6059	T6058	prep	of,cells
R3518	T6060	T6061	det	the,ganglia
R3519	T5908	T5909	amod	young,mice
R352	T793	T792	auxpass	is,caused
R3520	T6061	T6059	pobj	ganglia,of
R3521	T5909	T5907	pobj	mice,from
R3522	T5910	T5908	cc	and,young
R3523	T6062	T6061	amod	spiral,ganglia
R3524	T5911	T5908	conj	old,young
R3525	T6063	T6061	cc	but,ganglia
R3526	T6064	T6063	neg	not,but
R3527	T6065	T6066	det	the,preparation
R3528	T5912	T5909	nmod	Slc26a4,mice
R3529	T6066	T6061	conj	preparation,ganglia
R353	T794	T792	agent	by,caused
R3530	T6067	T6066	compound	stria,preparation
R3531	T6068	T6066	compound	vascularis,preparation
R3532	T5913	T5912	punct	-,Slc26a4
R3533	T6069	T6008	punct	.,used
R3534	T6071	T6072	det	The,number
R3535	T5914	T5912	punct	/,Slc26a4
R3536	T5915	T5912	punct	-,Slc26a4
R3537	T6072	T6073	nsubj	number,was
R3538	T5916	T5917	punct	(,0.18
R3539	T6074	T6072	prep	of,number
R354	T795	T794	pobj	mutations,by
R3540	T5917	T5902	parataxis	0.18,quantities
R3541	T6075	T6076	compound	KCNQ1,molecules
R3542	T6076	T6074	pobj	molecules,of
R3543	T6077	T6076	compound	mRNA,molecules
R3544	T5918	T5919	nmod	log,rRNA
R3545	T6078	T6072	prep	in,number
R3546	T6079	T6080	compound	stria,vascularis
R3547	T6080	T6078	pobj	vascularis,in
R3548	T6081	T6082	npadvmod	24,greater
R3549	T6082	T6073	acomp	greater,was
R355	T796	T795	prep	of,mutations
R3550	T5919	T5917	nsubj	rRNA,0.18
R3551	T6083	T6081	punct	-,24
R3552	T6084	T6081	advmod	fold,24
R3553	T6085	T6082	prep	than,greater
R3554	T5920	T5919	punct	(,rRNA
R3555	T6086	T6085	prep	in,than
R3556	T6087	T6088	amod	spiral,ganglia
R3557	T6088	T6086	pobj	ganglia,in
R3558	T5921	T5919	punct	),rRNA
R3559	T6089	T6073	punct	", ",was
R356	T797	T798	det	the,gene
R3560	T6090	T6073	cc	and,was
R3561	T5922	T5917	punct	=,0.18
R3562	T6091	T6092	det	the,number
R3563	T6092	T6093	nsubj	number,was
R3564	T6093	T6073	conj	was,was
R3565	T5923	T5917	quantmod	9.04,0.18
R3566	T6094	T6092	prep	of,number
R3567	T6095	T6096	compound	KCNQ4,molecules
R3568	T6096	T6094	pobj	molecules,of
R3569	T5924	T5917	punct	±,0.18
R357	T798	T796	pobj	gene,of
R3570	T6097	T6096	compound	mRNA,molecules
R3571	T6098	T6092	prep	in,number
R3572	T6099	T6100	amod	spiral,ganglia
R3573	T5925	T5926	punct	", ",4
R3574	T6100	T6098	pobj	ganglia,in
R3575	T6101	T6102	npadvmod	5,greater
R3576	T6102	T6093	acomp	greater,was
R3577	T5926	T5917	parataxis	4,0.18
R3578	T6103	T6101	punct	-,5
R3579	T6104	T6101	advmod	fold,5
R358	T799	T798	compound	PDS,gene
R3580	T6105	T6102	prep	than,greater
R3581	T5927	T5926	nsubj	n,4
R3582	T6106	T6105	prep	in,than
R3583	T6107	T6108	compound	stria,vascularis
R3584	T6108	T6106	pobj	vascularis,in
R3585	T5928	T5926	punct	=,4
R3586	T6109	T6093	punct	.,was
R3587	T6111	T6112	det	These,observations
R3588	T5929	T5917	cc	and,0.18
R3589	T6112	T6113	nsubj	observations,validate
R359	T800	T798	appos	SLC26A4,gene
R3590	T5930	T5931	quantmod	9.29,0.15
R3591	T6114	T6115	poss	our,measurements
R3592	T6115	T6113	dobj	measurements,validate
R3593	T6116	T6115	prep	of,measurements
R3594	T5931	T5917	conj	0.15,0.18
R3595	T6117	T6116	pobj	KCNJ10,of
R3596	T6118	T6117	cc	and,KCNJ10
R3597	T6119	T6117	conj	KCNQ1,KCNJ10
R3598	T6120	T6113	prep	by,validate
R3599	T6121	T6120	pcomp	demonstrating,by
R36	T270	T268	conj	goiter,deafness
R360	T801	T798	punct	", ",gene
R3600	T6122	T6123	mark	that,were
R3601	T5932	T5931	punct	±,0.15
R3602	T6123	T6121	ccomp	were,demonstrating
R3603	T6124	T6125	det	the,preparations
R3604	T6125	T6123	nsubj	preparations,were
R3605	T5933	T5934	punct	", ",6
R3606	T6126	T6125	amod	microdissected,preparations
R3607	T6127	T6125	prep	of,preparations
R3608	T6128	T6129	compound	stria,vascularis
R3609	T5934	T5931	parataxis	6,0.15
R361	T802	T803	dep	which,codes
R3610	T6129	T6127	pobj	vascularis,of
R3611	T6130	T6129	cc	and,vascularis
R3612	T6131	T6132	amod	spiral,ganglia
R3613	T5935	T5934	nsubj	n,6
R3614	T6132	T6129	conj	ganglia,vascularis
R3615	T6133	T6134	nummod	98,%
R3616	T6134	T6135	npadvmod	%,pure
R3617	T5936	T5934	punct	=,6
R3618	T6135	T6123	acomp	pure,were
R3619	T6136	T6134	cc	and,%
R362	T803	T798	relcl	codes,gene
R3620	T6137	T6138	nummod	78,%
R3621	T6147	T6148	amod	r,es
R3622	T6138	T6134	conj	%,%
R3623	T6139	T6123	punct	", ",were
R3624	T6140	T6123	advmod	respectively,were
R3625	T6141	T6113	punct	.,validate
R3626	T6149	T6148	acl	en,es
R3627	T6151	T6152	nmod	c,e
R3628	T6157	T6156	punct	,KCNJ10
R3629	T6154	T6153	prep	f,o
R363	T804	T803	prep	for,codes
R3630	T6164	T6165	compound	tria,vascularis
R3631	T6162	T6163	nsubj	s,supports
R3632	T6166	T6165	prep	of,vascularis
R3633	T6167	T6168	nmod	Slc26a4,mice
R3634	T6168	T6166	pobj	mice,of
R3635	T6250	T6251	amod	Histological,evaluation
R3636	T6251	T6252	nsubj	evaluation,revealed
R3637	T6169	T6167	punct	-,Slc26a4
R3638	T6253	T6251	prep	of,evaluation
R3639	T6254	T6253	pobj	cryosections,of
R364	T805	T806	det	the,pendrin
R3640	T6170	T6167	punct	/,Slc26a4
R3641	T6255	T6256	det	an,enlargement
R3642	T6256	T6252	dobj	enlargement,revealed
R3643	T6171	T6167	punct	-,Slc26a4
R3644	T6257	T6256	prep	of,enlargement
R3645	T6258	T6259	compound	scala,media
R3646	T6259	T6257	pobj	media,of
R3647	T6172	T6173	det	the,finding
R3648	T6260	T6256	prep	with,enlargement
R3649	T6261	T6262	det	a,bulging
R365	T806	T804	pobj	pendrin,for
R3650	T6262	T6260	pobj	bulging,with
R3651	T6263	T6262	amod	large,bulging
R3652	T6264	T6262	prep	of,bulging
R3653	T6173	T6163	dobj	finding,supports
R3654	T6265	T6266	poss	Reissner,membrane
R3655	T6266	T6264	pobj	membrane,of
R3656	T6267	T6265	case	's,Reissner
R3657	T6268	T6262	cc	and,bulging
R3658	T6174	T6175	mark	that,are
R3659	T6269	T6270	det	an,degeneration
R366	T807	T806	compound	protein,pendrin
R3660	T6270	T6262	conj	degeneration,bulging
R3661	T6271	T6270	amod	apparent,degeneration
R3662	T6175	T6173	acl	are,finding
R3663	T6272	T6270	prep	of,degeneration
R3664	T6273	T6274	det	the,organ
R3665	T6274	T6272	pobj	organ,of
R3666	T6176	T6177	amod	intermediate,cells
R3667	T6275	T6274	prep	of,organ
R3668	T6276	T6275	pobj	Corti,of
R3669	T6177	T6175	nsubj	cells,are
R367	T808	T809	punct	[,2
R3670	T6178	T6175	acomp	present,are
R3671	T6277	T6252	punct	", ",revealed
R3672	T6179	T6163	punct	.,supports
R3673	T6278	T6279	mark	as,described
R3674	T6279	T6252	advcl	described,revealed
R3675	T6280	T6279	advmod	earlier,described
R3676	T6181	T6182	nsubjpass	Expression,assessed
R3677	T6281	T6282	punct	[,14
R3678	T6282	T6252	parataxis	14,revealed
R3679	T6283	T6282	punct	],14
R368	T809	T803	parataxis	2,codes
R3680	T6183	T6181	prep	of,Expression
R3681	T6284	T6252	punct	.,revealed
R3682	T6286	T6287	compound	Tissue,height
R3683	T6287	T6288	nsubj	height,was
R3684	T6184	T6185	det	the,protein
R3685	T6289	T6287	prep	of,height
R3686	T6185	T6183	pobj	protein,of
R3687	T6290	T6291	compound	stria,vascularis
R3688	T6291	T6289	pobj	vascularis,of
R3689	T6292	T6288	acomp	normal,was
R369	T810	T809	punct	],2
R3690	T6186	T6185	compound	KCNJ10,protein
R3691	T6293	T6294	punct	(,Fig.
R3692	T6294	T6288	parataxis	Fig.,was
R3693	T6187	T6182	auxpass	was,assessed
R3694	T6295	T6294	nummod	5,Fig.
R3695	T6296	T6294	punct	),Fig.
R3696	T6297	T6288	punct	", ",was
R3697	T6188	T6182	prep	in,assessed
R3698	T6298	T6288	advcl	consistent,was
R3699	T6299	T6298	prep	with,consistent
R37	T271	T257	punct	", ",caused
R370	T811	T792	punct	.,caused
R3700	T6300	T6301	det	the,numbers
R3701	T6301	T6299	pobj	numbers,with
R3702	T6302	T6301	amod	similar,numbers
R3703	T6303	T6301	prep	of,numbers
R3704	T6189	T6190	amod	young,mice
R3705	T6304	T6305	compound	18S,molecules
R3706	T6305	T6303	pobj	molecules,of
R3707	T6306	T6305	compound	rRNA,molecules
R3708	T6190	T6188	pobj	mice,in
R3709	T6307	T6301	prep	in,numbers
R371	T813	T814	nsubj	Deafness,is
R3710	T6308	T6309	amod	isolated,preparations
R3711	T6309	T6307	pobj	preparations,in
R3712	T6191	T6190	nmod	Slc26a4,mice
R3713	T6310	T6311	punct	(,see
R3714	T6311	T6298	parataxis	see,consistent
R3715	T6312	T6311	advmod	above,see
R3716	T6192	T6191	punct	-,Slc26a4
R3717	T6313	T6311	punct	),see
R3718	T6314	T6288	punct	.,was
R3719	T6193	T6191	punct	/,Slc26a4
R372	T815	T814	acomp	congenital,is
R3720	T6316	T6317	det	The,absence
R3721	T6317	T6318	nsubj	absence,is
R3722	T6194	T6191	punct	-,Slc26a4
R3723	T6319	T6317	prep	of,absence
R3724	T6320	T6321	det	a,change
R3725	T6321	T6319	pobj	change,of
R3726	T6195	T6182	prep	by,assessed
R3727	T6322	T6321	prep	in,change
R3728	T6323	T6324	compound	tissue,height
R3729	T6324	T6322	pobj	height,in
R373	T816	T815	cc	and,congenital
R3730	T6196	T6197	amod	confocal,immunocytochemistry
R3731	T6325	T6318	acomp	consistent,is
R3732	T6326	T6325	prep	with,consistent
R3733	T6197	T6195	pobj	immunocytochemistry,by
R3734	T6327	T6328	det	the,presence
R3735	T6328	T6326	pobj	presence,with
R3736	T6329	T6328	prep	of,presence
R3737	T6198	T6182	punct	.,assessed
R3738	T6330	T6331	amod	intermediate,cells
R3739	T6331	T6329	pobj	cells,of
R374	T817	T818	advmod	generally,profound
R3740	T6332	T6333	punct	[,28
R3741	T6200	T6201	advmod	Interestingly,was
R3742	T6333	T6318	parataxis	28,is
R3743	T6334	T6333	punct	],28
R3744	T6335	T6318	punct	.,is
R3745	T6202	T6201	punct	", ",was
R3746	T6337	T6338	advmod	Further,observed
R3747	T6339	T6338	punct	", ",observed
R3748	T6340	T6338	nsubj	we,observed
R3749	T6203	T6201	nsubj	expression,was
R375	T818	T815	conj	profound,congenital
R3750	T6341	T6342	det	an,loss
R3751	T6342	T6338	dobj	loss,observed
R3752	T6343	T6342	amod	apparent,loss
R3753	T6204	T6203	prep	of,expression
R3754	T6344	T6342	prep	of,loss
R3755	T6345	T6346	compound	tissue,masses
R3756	T6205	T6206	det	the,protein
R3757	T6346	T6344	pobj	masses,of
R3758	T6206	T6204	pobj	protein,of
R3759	T6347	T6338	prep	in,observed
R376	T819	T820	mark	although,late
R3760	T6348	T6347	pobj	areas,in
R3761	T6349	T6350	dep	that,occupied
R3762	T6207	T6206	compound	KCNJ10,protein
R3763	T6350	T6348	relcl	occupied,areas
R3764	T6351	T6350	auxpass	are,occupied
R3765	T6352	T6350	advmod	normally,occupied
R3766	T6208	T6201	acomp	absent,was
R3767	T6353	T6354	advmod	primarily,by
R3768	T6354	T6350	agent	by,occupied
R3769	T6355	T6356	nmod	type,I
R377	T820	T818	advcl	late,profound
R3770	T6209	T6201	prep	in,was
R3771	T6356	T6357	nummod	I,fibrocytes
R3772	T6357	T6354	pobj	fibrocytes,by
R3773	T6210	T6211	compound	stria,vascularis
R3774	T6358	T6356	cc	and,I
R3775	T6359	T6356	conj	II,I
R3776	T6360	T6338	punct	.,observed
R3777	T6211	T6209	pobj	vascularis,in
R3778	T6362	T6363	amod	Spiral,prominence
R3779	T6212	T6201	cc	but,was
R378	T821	T820	advmod	sometimes,late
R3780	T6363	T6364	nsubj	prominence,was
R3781	T6365	T6363	prep	in,prominence
R3782	T6213	T6201	conj	normal,was
R3783	T6366	T6367	nmod	Slc26a4,mice
R3784	T6214	T6213	prep	in,normal
R3785	T6367	T6365	pobj	mice,in
R3786	T6368	T6366	punct	-,Slc26a4
R3787	T6215	T6216	amod	spiral,ganglia
R3788	T6369	T6366	punct	/,Slc26a4
R3789	T6370	T6366	punct	-,Slc26a4
R379	T822	T820	prep	in,late
R3790	T6371	T6372	advmod	less,prominent
R3791	T6216	T6214	pobj	ganglia,in
R3792	T6372	T6364	acomp	prominent,was
R3793	T6373	T6364	punct	", ",was
R3794	T6374	T6375	amod	spiral,ligament
R3795	T6217	T6218	punct	(,Fig.
R3796	T6375	T6376	nsubj	ligament,thinner
R3797	T6376	T6364	conj	thinner,was
R3798	T6218	T6213	parataxis	Fig.,normal
R3799	T6377	T6376	cc	and,thinner
R38	T272	T257	auxpass	is,caused
R380	T823	T822	pobj	onset,in
R3800	T6378	T6379	amod	spiral,limbus
R3801	T6379	T6380	nsubj	limbus,flatter
R3802	T6380	T6376	conj	flatter,thinner
R3803	T6219	T6218	nummod	4,Fig.
R3804	T6381	T6382	punct	(,Fig.
R3805	T6382	T6380	parataxis	Fig.,flatter
R3806	T6383	T6382	nummod	5,Fig.
R3807	T6384	T6382	punct	),Fig.
R3808	T6385	T6380	punct	.,flatter
R3809	T6220	T6218	punct	),Fig.
R381	T824	T815	cc	and,congenital
R3810	T6387	T6388	det	The,observation
R3811	T6388	T6389	nsubj	observation,is
R3812	T6221	T6201	punct	.,was
R3813	T6390	T6391	mark	that,lost
R3814	T6391	T6388	acl	lost,observation
R3815	T6392	T6393	compound	tissue,masses
R3816	T6223	T6224	det	The,absence
R3817	T6393	T6391	nsubjpass	masses,lost
R3818	T6394	T6391	auxpass	were,lost
R3819	T6395	T6391	prep	in,lost
R382	T825	T815	conj	provoked,congenital
R3820	T6396	T6397	det	the,region
R3821	T6224	T6225	nsubj	absence,is
R3822	T6397	T6395	pobj	region,in
R3823	T6398	T6399	amod	spiral,limbus
R3824	T6399	T6397	compound	limbus,region
R3825	T6226	T6224	prep	of,absence
R3826	T6400	T6389	acomp	consistent,is
R3827	T6401	T6400	prep	with,consistent
R3828	T6402	T6403	det	the,finding
R3829	T6403	T6401	pobj	finding,with
R383	T826	T825	agent	by,provoked
R3830	T6227	T6228	det	the,channel
R3831	T6404	T6403	prep	of,finding
R3832	T6405	T6406	det	a,number
R3833	T6406	T6404	pobj	number,of
R3834	T6228	T6226	pobj	channel,of
R3835	T6407	T6406	amod	reduced,number
R3836	T6408	T6406	prep	of,number
R3837	T6409	T6410	compound	18S,rRNA
R3838	T6229	T6228	nmod	KCNJ10,channel
R3839	T6410	T6411	compound	rRNA,molecules
R384	T827	T828	amod	light,injury
R3840	T6411	T6408	pobj	molecules,of
R3841	T6412	T6406	prep	in,number
R3842	T6230	T6228	nmod	K,channel
R3843	T6413	T6414	det	the,preparation
R3844	T6414	T6412	pobj	preparation,in
R3845	T6415	T6416	amod	spiral,ganglia
R3846	T6231	T6230	punct	+,K
R3847	T6416	T6414	compound	ganglia,preparation
R3848	T6417	T6418	punct	(,see
R3849	T6418	T6389	parataxis	see,is
R385	T828	T826	pobj	injury,by
R3850	T6232	T6224	prep	in,absence
R3851	T6419	T6418	advmod	above,see
R3852	T6420	T6418	punct	),see
R3853	T6421	T6389	punct	.,is
R3854	T6423	T6424	prep	In,observed
R3855	T6233	T6234	compound	stria,vascularis
R3856	T6425	T6423	pobj	addition,In
R3857	T6426	T6424	punct	", ",observed
R3858	T6427	T6424	nsubj	we,observed
R3859	T6428	T6429	det	an,degeneration
R386	T829	T828	compound	head,injury
R3860	T6429	T6424	dobj	degeneration,observed
R3861	T6234	T6232	pobj	vascularis,in
R3862	T6430	T6429	amod	apparent,degeneration
R3863	T6431	T6429	prep	of,degeneration
R3864	T6432	T6433	compound	stria,vascularis
R3865	T6235	T6225	acomp	sufficient,is
R3866	T6433	T6431	pobj	vascularis,of
R3867	T6434	T6429	punct	", ",degeneration
R3868	T6435	T6429	prep	including,degeneration
R3869	T6236	T6237	aux	to,explain
R387	T830	T814	punct	.,is
R3870	T6436	T6437	det	an,increase
R3871	T6437	T6435	pobj	increase,including
R3872	T6438	T6437	prep	in,increase
R3873	T6237	T6235	xcomp	explain,sufficient
R3874	T6439	T6438	pobj	pigmentation,in
R3875	T6440	T6437	cc	and,increase
R3876	T6441	T6442	det	an,pattern
R3877	T6238	T6239	det	the,loss
R3878	T6442	T6437	conj	pattern,increase
R3879	T6443	T6442	amod	irregular,pattern
R388	T832	T833	amod	Vestibular,dysfunction
R3880	T6239	T6237	dobj	loss,explain
R3881	T6444	T6442	prep	of,pattern
R3882	T6445	T6446	det	the,junctions
R3883	T6446	T6444	pobj	junctions,of
R3884	T6240	T6239	prep	of,loss
R3885	T6447	T6446	amod	tight,junctions
R3886	T6448	T6446	prep	of,junctions
R3887	T6449	T6450	amod	marginal,cells
R3888	T6241	T6242	det	the,potential
R3889	T6450	T6448	pobj	cells,of
R389	T833	T834	nsubj	dysfunction,is
R3890	T6451	T6452	punct	(,7
R3891	T6242	T6240	pobj	potential,of
R3892	T6243	T6242	amod	endocochlear,potential
R3893	T6452	T6424	parataxis	7,observed
R3894	T6244	T6245	punct	[,17
R3895	T6453	T6452	dep	Fig.,7
R3896	T6454	T6453	nummod	2c,Fig.
R3897	T6455	T6452	punct	", ",7
R3898	T6245	T6225	parataxis	17,is
R3899	T6456	T6452	nummod	6,7
R39	T273	T257	agent	by,caused
R390	T835	T834	acomp	uncommon,is
R3900	T6457	T6452	punct	",",7
R3901	T6458	T6452	punct	),7
R3902	T6246	T6245	punct	],17
R3903	T6459	T6424	punct	.,observed
R3904	T6247	T6225	punct	.,is
R3905	T6461	T6462	amod	Tight,junctions
R3906	T6462	T6463	nsubjpass	junctions,visualized
R3907	T6496	T6497	nsubjpass	s,i
R3908	T6464	T6463	auxpass	were,visualized
R3909	T6465	T6463	prep	by,visualized
R391	T836	T834	punct	.,is
R3910	T6466	T6467	compound	F,actin
R3911	T6467	T6469	compound	actin,expression
R3912	T6468	T6467	punct	-,actin
R3913	T6469	T6465	pobj	expression,by
R3914	T6470	T6463	punct	.,visualized
R3915	T6472	T6473	amod	Marginal,cells
R3916	T6473	T6474	nsubj	cells,appeared
R3917	T6475	T6476	aux	to,form
R3918	T6476	T6474	xcomp	form,appeared
R3919	T6477	T6478	det	a,layer
R392	T838	T839	nsubj	Goiter,is
R3920	T6478	T6476	dobj	layer,form
R3921	T6479	T6478	amod	continuous,layer
R3922	T6480	T6474	punct	.,appeared
R3923	T6531	T6532	nsubj	p,romi
R3924	T6485	T6483	advcl	ndr,e
R3925	T6487	T6486	amod	i,n
R3926	T6506	T6507	det	a,e
R3927	T6535	T6536	amod	e,n
R3928	T6507	T6505	pobj	e,f
R3929	T6508	T6507	amod	c,e
R393	T840	T839	acomp	variable,is
R3930	T6509	T6507	compound	e,e
R3931	T6546	T6545	auxpass	a,re
R3932	T6518	T6519	amod	al,s
R3933	T6520	T6519	compound	cell,s
R3934	T6521	T6517	prep	in,li
R3935	T6547	T6545	advcl	l,re
R3936	T6550	T6549	compound	c,a
R3937	T6564	T6565	amod	e,r
R3938	T6569	T6570	amod	basal,cells
R3939	T6570	T6568	nsubj	cells,form
R394	T841	T839	cc	and,is
R3940	T6571	T6570	punct	", ",cells
R3941	T6572	T6573	dep	which,are
R3942	T6573	T6570	relcl	are,cells
R3943	T6574	T6573	acomp	interconnected,are
R3944	T6575	T6573	prep	by,are
R3945	T6565	T6563	pobj	r,wh
R3946	T6576	T6577	amod	tight,junctions
R3947	T6577	T6575	pobj	junctions,by
R3948	T6668	T6664	prep	with,junction
R3949	T6578	T6568	punct	", ",form
R395	T842	T843	advmod	generally,develops
R3950	T6579	T6580	amod	additional,junctions
R3951	T6580	T6568	dobj	junctions,form
R3952	T6581	T6580	amod	tight,junctions
R3953	T6582	T6580	prep	with,junctions
R3954	T6583	T6584	nmod	surface,cells
R3955	T6584	T6582	pobj	cells,with
R3956	T6585	T6584	amod	epithelial,cells
R3957	T6586	T6587	punct	[,29
R3958	T6669	T6670	nmod	surface,cells
R3959	T6588	T6587	punct	],29
R396	T843	T839	conj	develops,is
R3960	T6670	T6668	pobj	cells,with
R3961	T6591	T6592	det	A,discontinuity
R3962	T6592	T6593	nsubj	discontinuity,explain
R3963	T6594	T6592	prep	of,discontinuity
R3964	T6595	T6596	det	this,junction
R3965	T6671	T6670	amod	epithelial,cells
R3966	T6596	T6594	pobj	junction,of
R3967	T6597	T6596	amod	complex,junction
R3968	T6598	T6592	prep	in,discontinuity
R3969	T6672	T6654	prep	in,revealed
R397	T844	T843	prep	around,develops
R3970	T6599	T6600	nmod	Slc26a4,mice
R3971	T6600	T6598	pobj	mice,in
R3972	T6601	T6599	punct	-,Slc26a4
R3973	T6673	T6674	nmod	Slc26a4,mice
R3974	T6602	T6599	punct	/,Slc26a4
R3975	T6603	T6599	punct	-,Slc26a4
R3976	T6604	T6593	aux	would,explain
R3977	T6674	T6672	pobj	mice,in
R3978	T6605	T6606	det	the,absence
R3979	T6606	T6593	dobj	absence,explain
R398	T845	T844	pobj	puberty,around
R3980	T6607	T6606	prep	of,absence
R3981	T6675	T6673	punct	-,Slc26a4
R3982	T6608	T6609	det	the,potential
R3983	T6609	T6607	pobj	potential,of
R3984	T6610	T6609	amod	endocochlear,potential
R3985	T6676	T6673	punct	/,Slc26a4
R3986	T6611	T6593	punct	.,explain
R3987	T6613	T6614	aux	To,evaluate
R3988	T6677	T6673	punct	-,Slc26a4
R3989	T6614	T6615	advcl	evaluate,determined
R399	T846	T847	punct	[,3
R3990	T6616	T6617	det	the,presence
R3991	T6678	T6654	punct	", ",revealed
R3992	T6617	T6614	dobj	presence,evaluate
R3993	T6618	T6617	prep	of,presence
R3994	T6619	T6620	det	this,connection
R3995	T6679	T6680	mark	as,observed
R3996	T6620	T6618	pobj	connection,of
R3997	T6621	T6615	punct	", ",determined
R3998	T6680	T6654	advcl	observed,revealed
R3999	T6622	T6615	nsubj	we,determined
R40	T274	T273	pobj	mutations,by
R400	T847	T843	parataxis	3,develops
R4000	T6623	T6615	prep	by,determined
R4001	T6624	T6625	amod	confocal,immunocytochemistry
R4002	T6681	T6680	prep	in,observed
R4003	T6625	T6623	pobj	immunocytochemistry,by
R4004	T6626	T6627	det	the,expression
R4005	T6627	T6615	dobj	expression,determined
R4006	T6682	T6683	amod	normal,mice
R4007	T6628	T6627	prep	of,expression
R4008	T6629	T6628	pobj	ZO,of
R4009	T6683	T6681	pobj	mice,in
R401	T848	T847	punct	],3
R4010	T6630	T6629	punct	-,ZO
R4011	T6684	T6685	punct	(,7
R4012	T6631	T6629	nummod	1,ZO
R4013	T6632	T6628	cc	and,of
R4014	T6633	T6628	conj	of,of
R4015	T6634	T6635	compound	F,actin
R4016	T6635	T6633	pobj	actin,of
R4017	T6636	T6635	punct	-,actin
R4018	T6685	T6683	parataxis	7,mice
R4019	T6637	T6633	punct	", ",of
R402	T849	T839	punct	.,is
R4020	T6638	T6639	dep	which,associate
R4021	T6686	T6687	nmod	Fig.,1f
R4022	T6639	T6633	conj	associate,of
R4023	T6640	T6639	prep	with,associate
R4024	T6641	T6642	amod	tight,complexes
R4025	T6687	T6685	dep	1f,7
R4026	T6642	T6640	pobj	complexes,with
R4027	T6643	T6642	compound	junction,complexes
R4028	T6644	T6615	punct	.,determined
R4029	T6688	T6687	nummod	1c,1f
R403	T851	T852	det	The,cause
R4030	T6646	T6647	nmod	ZO,expression
R4031	T6647	T6654	nsubj	expression,revealed
R4032	T6689	T6687	punct	",",1f
R4033	T6648	T6646	punct	-,ZO
R4034	T6649	T6646	nummod	1,ZO
R4035	T6650	T6646	cc	and,ZO
R4036	T6690	T6687	nummod	1d,1f
R4037	T6651	T6652	compound	F,actin
R4038	T6652	T6646	conj	actin,ZO
R4039	T6653	T6652	punct	-,actin
R404	T852	T853	nsubj	cause,appears
R4040	T6691	T6687	punct	",",1f
R4041	T6655	T6656	det	a,layer
R4042	T6656	T6654	dobj	layer,revealed
R4043	T6692	T6687	nummod	1e,1f
R4044	T6657	T6656	amod	continuous,layer
R4045	T6658	T6656	prep	of,layer
R4046	T6659	T6660	amod	basal,cells
R4047	T6693	T6687	punct	",",1f
R4048	T6660	T6658	pobj	cells,of
R4049	T6661	T6656	punct	", ",layer
R405	T854	T852	prep	of,cause
R4050	T6662	T6656	prep	including,layer
R4051	T6694	T6685	punct	",",7
R4052	T6663	T6664	det	a,junction
R4053	T6664	T6662	pobj	junction,including
R4054	T6665	T6664	prep	of,junction
R4055	T6695	T6685	nummod	6,7
R4056	T6666	T6667	amod	basal,cells
R4057	T6667	T6665	pobj	cells,of
R4058	T6696	T6685	punct	",",7
R4059	T6697	T6685	punct	),7
R406	T855	T854	pobj	goiter,of
R4060	T6698	T6654	punct	.,revealed
R4061	T6700	T6701	det	These,observations
R4062	T6774	T6772	dobj	concentration,maintain
R4063	T6701	T6702	nsubj	observations,make
R4064	T6775	T6774	nmod	K,concentration
R4065	T6703	T6704	nsubj	it,unlikely
R4066	T6776	T6775	punct	+,K
R4067	T6777	T6765	punct	", ",be
R4068	T6778	T6765	advmod	however,be
R4069	T6779	T6765	punct	", ",be
R407	T856	T857	aux	to,be
R4070	T6780	T6765	aux	may,be
R4071	T6781	T6765	acomp	less,be
R4072	T6782	T6781	prep	than,less
R4073	T6704	T6702	ccomp	unlikely,make
R4074	T6783	T6782	amod	necessary,than
R4075	T6784	T6765	prep	in,be
R4076	T6785	T6786	nmod	Slc26a4,mice
R4077	T6705	T6706	mark	that,is
R4078	T6786	T6784	pobj	mice,in
R4079	T6787	T6785	punct	+,Slc26a4
R408	T857	T853	xcomp	be,appears
R4080	T6788	T6785	punct	/,Slc26a4
R4081	T6706	T6704	advcl	is,unlikely
R4082	T6789	T6785	punct	+,Slc26a4
R4083	T6790	T6765	prep	given,be
R4084	T6791	T6792	det	the,numbers
R4085	T6792	T6790	pobj	numbers,given
R4086	T6707	T6708	det	the,cause
R4087	T6793	T6792	amod	reduced,numbers
R4088	T6794	T6792	prep	of,numbers
R4089	T6708	T6706	nsubj	cause,is
R409	T858	T859	det	an,impairment
R4090	T6795	T6796	amod	sensory,cells
R4091	T6796	T6794	pobj	cells,of
R4092	T6797	T6796	compound	hair,cells
R4093	T6709	T6708	amod	primary,cause
R4094	T6798	T6796	punct	", ",cells
R4095	T6799	T6800	dep	which,provide
R4096	T6710	T6708	prep	of,cause
R4097	T6800	T6796	relcl	provide,cells
R4098	T6711	T6712	det	the,loss
R4099	T6801	T6802	det	a,pathway
R41	T275	T274	prep	of,mutations
R410	T859	T857	attr	impairment,be
R4100	T6802	T6800	dobj	pathway,provide
R4101	T6803	T6802	amod	major,pathway
R4102	T6712	T6710	pobj	loss,of
R4103	T6804	T6802	prep	for,pathway
R4104	T6805	T6806	nmod	K,exit
R4105	T6806	T6804	pobj	exit,for
R4106	T6807	T6805	punct	+,K
R4107	T6808	T6806	prep	from,exit
R4108	T6713	T6712	prep	of,loss
R4109	T6809	T6808	pobj	endolymph,from
R411	T860	T859	prep	of,impairment
R4110	T6810	T6811	punct	[,31
R4111	T6811	T6765	parataxis	31,be
R4112	T6714	T6715	det	the,potential
R4113	T6812	T6811	nummod	30,31
R4114	T6813	T6811	punct	",",31
R4115	T6814	T6811	punct	],31
R4116	T6715	T6713	pobj	potential,of
R4117	T6815	T6765	punct	.,be
R4118	T6817	T6818	prep	In,measured
R4119	T6716	T6715	amod	endocochlear,potential
R412	T861	T862	compound	iodide,fixation
R4120	T6819	T6817	pobj	order,In
R4121	T6820	T6821	aux	to,substantiate
R4122	T6821	T6819	acl	substantiate,order
R4123	T6822	T6823	det	the,view
R4124	T6823	T6821	dobj	view,substantiate
R4125	T6717	T6718	det	a,compromise
R4126	T6824	T6825	mark	that,secretes
R4127	T6825	T6823	acl	secretes,view
R4128	T6826	T6827	compound	stria,vascularis
R4129	T6827	T6825	nsubj	vascularis,secretes
R413	T862	T860	pobj	fixation,of
R4130	T6718	T6706	attr	compromise,is
R4131	T6828	T6827	prep	in,vascularis
R4132	T6719	T6718	prep	in,compromise
R4133	T6829	T6828	pobj	Slc26a4,in
R4134	T6830	T6829	punct	-,Slc26a4
R4135	T6831	T6829	punct	/,Slc26a4
R4136	T6720	T6721	det	the,barrier
R4137	T6832	T6829	punct	-,Slc26a4
R4138	T6833	T6825	dobj	K,secretes
R4139	T6834	T6833	punct	+,K
R414	T863	T859	prep	in,impairment
R4140	T6721	T6719	pobj	barrier,in
R4141	T6835	T6818	punct	", ",measured
R4142	T6836	T6818	nsubj	we,measured
R4143	T6837	T6838	det	the,magnitude
R4144	T6722	T6723	amod	basal,cell
R4145	T6838	T6818	dobj	magnitude,measured
R4146	T6839	T6838	prep	of,magnitude
R4147	T6840	T6841	det	the,current
R4148	T6723	T6721	compound	cell,barrier
R4149	T6841	T6839	pobj	current,of
R415	T864	T865	det	the,lumen
R4150	T6842	T6843	npadvmod	bumetanide,sensitive
R4151	T6843	T6841	amod	sensitive,current
R4152	T6724	T6702	punct	.,make
R4153	T6844	T6843	punct	-,sensitive
R4154	T6845	T6841	acl	exiting,current
R4155	T6846	T6847	compound	stria,vascularis
R4156	T6726	T6727	det	The,observation
R4157	T6847	T6845	dobj	vascularis,exiting
R4158	T6848	T6845	prep	across,exiting
R4159	T6849	T6850	det	the,membrane
R416	T865	T863	pobj	lumen,in
R4160	T6850	T6848	pobj	membrane,across
R4161	T6727	T6728	nsubj	observation,suggests
R4162	T6851	T6850	amod	apical,membrane
R4163	T6852	T6850	prep	of,membrane
R4164	T6853	T6854	amod	strial,cells
R4165	T6729	T6730	mark	that,were
R4166	T6854	T6852	pobj	cells,of
R4167	T6855	T6854	amod	marginal,cells
R4168	T6856	T6818	cc	and,measured
R4169	T6857	T6818	conj	determined,measured
R417	T866	T865	amod	follicular,lumen
R4170	T6730	T6727	acl	were,observation
R4171	T6858	T6859	det	the,expression
R4172	T6859	T6857	dobj	expression,determined
R4173	T6860	T6859	prep	of,expression
R4174	T6861	T6862	det	the,proteins
R4175	T6862	T6860	pobj	proteins,of
R4176	T6863	T6862	appos	KCNQ1,proteins
R4177	T6731	T6732	amod	endolymphatic,concentrations
R4178	T6864	T6863	punct	", ",KCNQ1
R4179	T6865	T6863	conj	KCNE1,KCNQ1
R418	T867	T857	prep	due,be
R4180	T6866	T6865	cc	and,KCNE1
R4181	T6732	T6730	nsubj	concentrations,were
R4182	T6867	T6865	conj	SLC12A2,KCNE1
R4183	T6868	T6862	punct	", ",proteins
R4184	T6869	T6870	dep	which,are
R4185	T6733	T6731	cc	and,endolymphatic
R4186	T6870	T6862	relcl	are,proteins
R4187	T6871	T6870	acomp	essential,are
R4188	T6872	T6871	prep	for,essential
R4189	T6734	T6731	conj	perilymphatic,endolymphatic
R419	T868	T867	pcomp	to,due
R4190	T6873	T6874	nmod	K,secretion
R4191	T6874	T6872	pobj	secretion,for
R4192	T6875	T6873	punct	+,K
R4193	T6735	T6732	nmod	K,concentrations
R4194	T6876	T6877	punct	[,33
R4195	T6877	T6870	parataxis	33,are
R4196	T6878	T6877	nummod	20,33
R4197	T6736	T6735	punct	+,K
R4198	T6879	T6877	punct	",",33
R4199	T6737	T6730	acomp	normal,were
R42	T276	T275	pobj	SLC26A4,of
R420	T869	T870	det	a,rate
R4200	T6738	T6730	prep	in,were
R4201	T6880	T6877	nummod	32,33
R4202	T6881	T6877	punct	",",33
R4203	T6739	T6740	nmod	Slc26a4,mice
R4204	T6882	T6877	punct	],33
R4205	T6740	T6738	pobj	mice,in
R4206	T6883	T6860	punct	", ",of
R4207	T6741	T6739	punct	-,Slc26a4
R4208	T6884	T6860	cc	and,of
R4209	T6885	T6860	conj	of,of
R421	T870	T867	pobj	rate,due
R4210	T6886	T6887	nmod	GJB2,Cx26
R4211	T6742	T6739	punct	/,Slc26a4
R4212	T6887	T6885	pobj	Cx26,of
R4213	T6888	T6887	punct	(,Cx26
R4214	T6743	T6739	punct	-,Slc26a4
R4215	T6889	T6887	punct	),Cx26
R4216	T6890	T6887	punct	", ",Cx26
R4217	T6891	T6892	dep	which,thought
R4218	T6744	T6745	mark	that,was
R4219	T6892	T6887	relcl	thought,Cx26
R422	T871	T870	amod	reduced,rate
R4220	T6893	T6892	auxpass	is,thought
R4221	T6745	T6728	ccomp	was,suggests
R4222	T6894	T6895	aux	to,contribute
R4223	T6895	T6892	xcomp	contribute,thought
R4224	T6746	T6747	compound	stria,vascularis
R4225	T6896	T6895	prep	to,contribute
R4226	T6897	T6898	nmod	K,cycling
R4227	T6898	T6896	pobj	cycling,to
R4228	T6899	T6897	punct	+,K
R4229	T6900	T6901	punct	[,23
R423	T872	T870	prep	of,rate
R4230	T6901	T6857	parataxis	23,determined
R4231	T6747	T6745	nsubj	vascularis,was
R4232	T6902	T6901	punct	],23
R4233	T6903	T6818	punct	.,measured
R4234	T6748	T6745	acomp	able,was
R4235	T6905	T6906	nmod	K,secretion
R4236	T6906	T6908	nsubjpass	secretion,known
R4237	T6907	T6905	punct	+,K
R4238	T6749	T6750	aux	to,secrete
R4239	T6909	T6908	auxpass	is,known
R424	T873	T874	compound	iodide,transport
R4240	T6750	T6748	xcomp	secrete,able
R4241	T6910	T6911	aux	to,be
R4242	T6911	T6908	xcomp	be,known
R4243	T6751	T6750	dobj	K,secrete
R4244	T6912	T6911	acomp	sensitive,be
R4245	T6913	T6912	prep	to,sensitive
R4246	T6914	T6915	compound	10,5
R4247	T6752	T6751	punct	+,K
R4248	T6915	T6917	nummod	5,M
R4249	T6916	T6915	punct	-,5
R425	T874	T872	pobj	transport,of
R4250	T6917	T6918	compound	M,bumetanide
R4251	T6753	T6745	prep	in,was
R4252	T6918	T6913	pobj	bumetanide,to
R4253	T6919	T6918	punct	", ",bumetanide
R4254	T6920	T6921	det	a,diuretic
R4255	T6754	T6753	pobj	spite,in
R4256	T6921	T6918	appos	diuretic,bumetanide
R4257	T6922	T6921	compound	loop,diuretic
R4258	T6923	T6921	punct	-,diuretic
R4259	T6755	T6754	prep	of,spite
R426	T875	T874	prep	across,transport
R4260	T6924	T6925	dep	that,inhibits
R4261	T6925	T6921	relcl	inhibits,diuretic
R4262	T6926	T6927	det	the,cotransporter
R4263	T6756	T6757	det	the,signs
R4264	T6927	T6925	dobj	cotransporter,inhibits
R4265	T6928	T6927	nmod	Na,cotransporter
R4266	T6757	T6755	pobj	signs,of
R4267	T6929	T6928	punct	+,Na
R4268	T6930	T6928	punct	/,Na
R4269	T6931	T6928	appos	2Cl,Na
R427	T876	T877	det	the,membrane
R4270	T6758	T6757	amod	apparent,signs
R4271	T6932	T6931	punct	-,2Cl
R4272	T6933	T6928	punct	/,Na
R4273	T6934	T6928	appos	K,Na
R4274	T6759	T6757	prep	of,signs
R4275	T6935	T6934	punct	+,K
R4276	T6936	T6927	appos	SLC12A2,cotransporter
R4277	T6937	T6938	punct	[,26
R4278	T6938	T6925	parataxis	26,inhibits
R4279	T6939	T6938	punct	],26
R428	T877	T875	pobj	membrane,across
R4280	T6940	T6908	punct	.,known
R4281	T6760	T6759	pobj	degeneration,of
R4282	T6942	T6943	npadvmod	Bumetanide,sensitive
R4283	T6943	T6945	amod	sensitive,currents
R4284	T6761	T6728	punct	.,suggests
R4285	T6944	T6943	punct	-,sensitive
R4286	T6945	T6946	nsubjpass	currents,found
R4287	T6763	T6764	det	The,rate
R4288	T6947	T6946	auxpass	were,found
R4289	T6948	T6946	prep	in,found
R429	T878	T877	amod	apical,membrane
R4290	T6949	T6950	preconj	both,Slc26a4
R4291	T6950	T6951	nmod	Slc26a4,mice
R4292	T6764	T6765	nsubj	rate,be
R4293	T6951	T6948	pobj	mice,in
R4294	T6952	T6950	punct	+,Slc26a4
R4295	T6953	T6950	punct	/,Slc26a4
R4296	T6766	T6764	prep	of,rate
R4297	T6954	T6950	punct	+,Slc26a4
R4298	T6955	T6950	cc	and,Slc26a4
R4299	T6956	T6950	conj	Slc26a4,Slc26a4
R43	T277	T276	punct	", ",SLC26A4
R430	T879	T877	prep	of,membrane
R4300	T6957	T6956	punct	-,Slc26a4
R4301	T6767	T6768	nmod	K,secretion
R4302	T6958	T6956	punct	/,Slc26a4
R4303	T6959	T6956	punct	-,Slc26a4
R4304	T6960	T6961	mark	although,was
R4305	T6768	T6766	pobj	secretion,of
R4306	T6961	T6946	advcl	was,found
R4307	T6962	T6963	det	the,magnitude
R4308	T6963	T6961	nsubj	magnitude,was
R4309	T6769	T6767	punct	+,K
R431	T880	T881	amod	thyroid,gland
R4310	T6964	T6963	prep	of,magnitude
R4311	T6965	T6966	det	the,current
R4312	T6966	T6964	pobj	current,of
R4313	T6770	T6764	amod	necessary,rate
R4314	T6967	T6968	advmod	significantly,smaller
R4315	T6771	T6772	aux	to,maintain
R4316	T6968	T6961	acomp	smaller,was
R4317	T6969	T6961	prep	in,was
R4318	T6772	T6770	xcomp	maintain,necessary
R4319	T6970	T6971	nmod	Slc26a4,mice
R432	T881	T882	nmod	gland,cells
R4320	T6971	T6969	pobj	mice,in
R4321	T6773	T6774	amod	endolymphatic,concentration
R4322	T6972	T6970	punct	-,Slc26a4
R4323	T6973	T6970	punct	/,Slc26a4
R4324	T6974	T6970	punct	-,Slc26a4
R4325	T6985	T6976	punct	=,4
R4326	T6975	T6976	punct	(,4
R4327	T6976	T6961	parataxis	4,was
R4328	T6977	T6978	quantmod	22,6
R4329	T6978	T6980	nummod	6,μA
R433	T882	T879	pobj	cells,of
R4330	T6979	T6978	punct	±,6
R4331	T6980	T6976	dep	μA,4
R4332	T6981	T6982	punct	/,cm2
R4333	T6982	T6980	prep	cm2,μA
R4334	T6983	T6976	punct	", ",4
R4335	T6984	T6976	nsubj	n,4
R4336	T6986	T6976	cc	versus,4
R4337	T6987	T6988	quantmod	14,2
R4338	T6988	T6990	nummod	2,μA
R4339	T7091	T7092	amod	vestibular,cells
R434	T883	T882	amod	epithelial,cells
R4340	T6989	T6988	punct	±,2
R4341	T6990	T6991	dep	μA,4
R4342	T6991	T6976	conj	4,4
R4343	T7092	T7090	pobj	cells,in
R4344	T6992	T6993	punct	/,cm2
R4345	T7093	T7092	amod	dark,cells
R4346	T7094	T7095	punct	(,Fig.
R4347	T6993	T6990	prep	cm2,μA
R4348	T7095	T7082	parataxis	Fig.,observed
R4349	T7096	T7095	nummod	1i,Fig.
R435	T884	T885	punct	[,4
R4350	T6994	T6991	punct	", ",4
R4351	T7097	T7095	punct	),Fig.
R4352	T7098	T7082	punct	.,observed
R4353	T6995	T6991	nsubj	n,4
R4354	T7100	T7101	nsubj	Expression,is
R4355	T7102	T7100	prep	of,Expression
R4356	T6996	T6991	punct	=,4
R4357	T7103	T7104	compound	KCNQ1,protein
R4358	T7104	T7102	pobj	protein,of
R4359	T7105	T7100	prep	in,Expression
R436	T885	T857	parataxis	4,be
R4360	T6997	T6991	punct	),4
R4361	T7106	T7107	compound	stria,vascularis
R4362	T7107	T7105	pobj	vascularis,in
R4363	T6998	T6946	punct	.,found
R4364	T7108	T7107	prep	of,vascularis
R4365	T7109	T7110	nmod	Slc26a4,mice
R4366	T7110	T7108	pobj	mice,of
R4367	T7000	T7001	nsubjpass	KCNQ1,co-localized
R4368	T7111	T7109	punct	-,Slc26a4
R4369	T7112	T7109	punct	/,Slc26a4
R437	T886	T885	punct	],4
R4370	T7113	T7109	punct	-,Slc26a4
R4371	T7002	T7000	cc	and,KCNQ1
R4372	T7114	T7101	acomp	consistent,is
R4373	T7115	T7114	prep	with,consistent
R4374	T7116	T7117	det	the,finding
R4375	T7117	T7115	pobj	finding,with
R4376	T7003	T7000	conj	KCNE1,KCNQ1
R4377	T7118	T7117	prep	of,finding
R4378	T7119	T7120	compound	KCNQ1,mRNA
R4379	T7004	T7000	punct	", ",KCNQ1
R438	T887	T853	punct	.,appears
R4380	T7120	T7121	compound	mRNA,expression
R4381	T7121	T7118	pobj	expression,of
R4382	T7122	T7123	punct	(,Fig.
R4383	T7123	T7101	parataxis	Fig.,is
R4384	T7124	T7123	nummod	3,Fig.
R4385	T7125	T7123	punct	),Fig.
R4386	T7005	T7000	appos	subunits,KCNQ1
R4387	T7126	T7101	punct	.,is
R4388	T7128	T7129	det	These,observations
R4389	T7006	T7005	prep	of,subunits
R439	T889	T890	det	A,test
R4390	T7129	T7130	nsubj	observations,make
R4391	T7131	T7132	nsubj	it,unlikely
R4392	T7007	T7008	det	the,channel
R4393	T7132	T7130	ccomp	unlikely,make
R4394	T7133	T7134	mark	that,is
R4395	T7008	T7006	pobj	channel,of
R4396	T7009	T7008	amod	secretory,channel
R4397	T7134	T7132	advcl	is,unlikely
R4398	T7010	T7008	nmod	K,channel
R4399	T7135	T7136	det	the,cause
R44	T278	T279	dep	which,codes
R440	T890	T894	nsubj	test,appear
R4400	T7136	T7134	nsubj	cause,is
R4401	T7137	T7136	amod	primary,cause
R4402	T7011	T7010	punct	+,K
R4403	T7138	T7136	prep	of,cause
R4404	T7139	T7140	det	the,loss
R4405	T7012	T7001	punct	", ",co-localized
R4406	T7140	T7138	pobj	loss,of
R4407	T7141	T7140	prep	of,loss
R4408	T7142	T7143	det	the,potential
R4409	T7013	T7001	auxpass	were,co-localized
R441	T891	T890	amod	positive,test
R4410	T7143	T7141	pobj	potential,of
R4411	T7144	T7143	amod	endocochlear,potential
R4412	T7145	T7146	det	a,compromise
R4413	T7014	T7001	prep	in,co-localized
R4414	T7146	T7134	attr	compromise,is
R4415	T7147	T7146	prep	of,compromise
R4416	T7015	T7016	det	the,membrane
R4417	T7148	T7149	nmod	K,secretion
R4418	T7149	T7147	pobj	secretion,of
R4419	T7016	T7014	pobj	membrane,in
R442	T892	T893	compound	perchlorate,discharge
R4420	T7150	T7148	punct	+,K
R4421	T7151	T7146	prep	by,compromise
R4422	T7152	T7153	amod	strial,marginal
R4423	T7153	T7154	amod	marginal,cells
R4424	T7017	T7016	amod	apical,membrane
R4425	T7154	T7151	pobj	cells,by
R4426	T7155	T7146	cc	or,compromise
R4427	T7018	T7016	prep	of,membrane
R4428	T7156	T7157	det	a,compromise
R4429	T7157	T7146	conj	compromise,compromise
R443	T893	T890	compound	discharge,test
R4430	T7158	T7157	prep	of,compromise
R4431	T7019	T7020	amod	strial,marginal
R4432	T7159	T7160	compound	gap,junction
R4433	T7160	T7161	npadvmod	junction,mediated
R4434	T7161	T7162	amod	mediated,cycling
R4435	T7162	T7158	pobj	cycling,of
R4436	T7163	T7162	nmod	K,cycling
R4437	T7164	T7163	punct	+,K
R4438	T7020	T7021	amod	marginal,cells
R4439	T7165	T7130	punct	.,make
R444	T895	T890	cc	and,test
R4440	T7021	T7018	pobj	cells,of
R4441	T7022	T7001	punct	;,co-localized
R4442	T7023	T7024	det	the,cotransporter
R4443	T7024	T7033	nsubjpass	cotransporter,found
R4444	T7025	T7024	nmod	Na,cotransporter
R4445	T7026	T7025	punct	+,Na
R4446	T7027	T7025	punct	/,Na
R4447	T7028	T7025	appos	2Cl,Na
R4448	T7029	T7028	punct	-,2Cl
R4449	T7030	T7025	punct	/,Na
R445	T896	T897	det	an,aqueduct
R4450	T7031	T7025	appos	K,Na
R4451	T7032	T7031	punct	+,K
R4452	T7033	T7001	conj	found,co-localized
R4453	T7034	T7024	appos	SLC12A2,cotransporter
R4454	T7035	T7024	punct	", ",cotransporter
R4455	T7036	T7037	dep	which,located
R4456	T7037	T7024	relcl	located,cotransporter
R4457	T7038	T7037	auxpass	is,located
R4458	T7039	T7037	prep	in,located
R4459	T7040	T7041	det	the,membrane
R446	T897	T890	conj	aqueduct,test
R4460	T7041	T7039	pobj	membrane,in
R4461	T7042	T7041	amod	basolateral,membrane
R4462	T7043	T7041	prep	of,membrane
R4463	T7044	T7045	amod	strial,cells
R4464	T7045	T7043	pobj	cells,of
R4465	T7046	T7045	amod	marginal,cells
R4466	T7047	T7033	punct	", ",found
R4467	T7048	T7033	auxpass	was,found
R4468	T7049	T7033	prep	in,found
R4469	T7050	T7051	compound	stria,vascularis
R447	T898	T897	amod	enlarged,aqueduct
R4470	T7051	T7049	pobj	vascularis,in
R4471	T7052	T7033	punct	;,found
R4472	T7053	T7033	cc	and,found
R4473	T7054	T7055	det	the,protein
R4474	T7055	T7058	nsubjpass	protein,found
R4475	T7056	T7057	compound	gap,junction
R4476	T7057	T7055	compound	junction,protein
R4477	T7058	T7033	conj	found,found
R4478	T7059	T7055	appos	GJB2,protein
R4479	T7060	T7058	auxpass	was,found
R448	T899	T897	amod	vestibular,aqueduct
R4480	T7061	T7058	prep	in,found
R4481	T7062	T7063	amod	spiral,ligament
R4482	T7063	T7061	pobj	ligament,in
R4483	T7064	T7063	prep	of,ligament
R4484	T7065	T7066	nmod	Slc26a4,mice
R4485	T7066	T7064	pobj	mice,of
R4486	T7067	T7065	punct	+,Slc26a4
R4487	T7068	T7065	punct	/,Slc26a4
R4488	T7069	T7065	punct	+,Slc26a4
R4489	T7070	T7065	cc	and,Slc26a4
R449	T900	T901	aux	to,be
R4490	T7071	T7065	conj	Slc26a4,Slc26a4
R4491	T7072	T7071	punct	-,Slc26a4
R4492	T7073	T7071	punct	/,Slc26a4
R4493	T7074	T7071	punct	-,Slc26a4
R4494	T7075	T7076	punct	(,Fig.
R4495	T7076	T7058	parataxis	Fig.,found
R4496	T7077	T7076	nummod	8,Fig.
R4497	T7078	T7076	punct	),Fig.
R4498	T7079	T7058	punct	.,found
R4499	T7081	T7082	nsubjpass	Co-localization,observed
R45	T279	T276	relcl	codes,SLC26A4
R450	T901	T894	xcomp	be,appear
R4500	T7083	T7081	prep	of,Co-localization
R4501	T7084	T7085	nmod	KCNQ1,proteins
R4502	T7085	T7083	pobj	proteins,of
R4503	T7086	T7084	cc	and,KCNQ1
R4504	T7087	T7084	conj	KCNE1,KCNQ1
R4505	T7088	T7082	auxpass	was,observed
R4506	T7089	T7082	advmod	also,observed
R4507	T7090	T7082	prep	in,observed
R451	T902	T903	det	the,signs
R4510	T7393	T7394	nsubj	We,described
R4511	T7395	T7394	dobj	locations,described
R4512	T7396	T7395	prep	of,locations
R4513	T7397	T7398	compound	pendrin,expression
R4514	T7398	T7396	pobj	expression,of
R4515	T7399	T7395	prep	in,locations
R4516	T7400	T7401	det	the,cochlea
R4517	T7401	T7399	pobj	cochlea,in
R4518	T7402	T7401	cc	and,cochlea
R4519	T7403	T7404	amod	vestibular,labyrinth
R452	T903	T901	attr	signs,be
R4520	T7404	T7401	conj	labyrinth,cochlea
R4521	T7405	T7394	cc	and,described
R4522	T7406	T7394	conj	detected,described
R4523	T7407	T7408	amod	normal,concentrations
R4524	T7408	T7406	dobj	concentrations,detected
R4525	T7409	T7408	amod	endolymphatic,concentrations
R4526	T7410	T7408	nmod	K,concentrations
R4527	T7411	T7410	punct	+,K
R4528	T7412	T7406	prep	in,detected
R4529	T7413	T7412	pobj	spite,in
R453	T904	T905	advmod	most,reliable
R4530	T7414	T7413	prep	of,spite
R4531	T7415	T7416	det	an,enlargement
R4532	T7416	T7414	pobj	enlargement,of
R4533	T7417	T7416	prep	of,enlargement
R4534	T7418	T7419	det	this,compartment
R4535	T7419	T7417	pobj	compartment,of
R4536	T7420	T7419	compound	fluid,compartment
R4537	T7421	T7394	punct	.,described
R4538	T7423	T7424	nsubj	We,found
R4539	T7425	T7426	mark	that,lack
R454	T905	T903	amod	reliable,signs
R4540	T7426	T7424	ccomp	lack,found
R4541	T7427	T7428	nmod	Slc26a4,mice
R4542	T7428	T7426	nsubj	mice,lack
R4543	T7429	T7427	punct	-,Slc26a4
R4544	T7430	T7427	punct	/,Slc26a4
R4545	T7431	T7427	punct	-,Slc26a4
R4546	T7432	T7433	det	the,potential
R4547	T7433	T7426	dobj	potential,lack
R4548	T7434	T7433	amod	endocochlear,potential
R4549	T7435	T7436	mark	because,express
R455	T906	T903	amod	clinical,signs
R4550	T7436	T7426	advcl	express,lack
R4551	T7437	T7436	nsubj	they,express
R4552	T7438	T7436	aux	do,express
R4553	T7439	T7436	neg	not,express
R4554	T7440	T7441	compound	KCNJ10,protein
R4555	T7441	T7436	dobj	protein,express
R4556	T7442	T7424	punct	.,found
R4557	T7444	T7445	amod	Intermediate,cells
R4558	T7445	T7446	nsubj	cells,protect
R4559	T7447	T7448	compound	stria,vascularis
R456	T907	T903	prep	of,signs
R4560	T7448	T7446	dobj	vascularis,protect
R4561	T7449	T7446	prep	by,protect
R4562	T7450	T7449	pcomp	converting,by
R4563	T7451	T7450	dobj	CO2,converting
R4564	T7452	T7450	prep	to,converting
R4565	T7453	T7452	pobj	HCO3,to
R4566	T7454	T7453	punct	-,HCO3
R4567	T7455	T7449	cc	and,by
R4568	T7456	T7449	conj	by,by
R4569	T7457	T7456	pcomp	detoxifying,by
R457	T908	T909	compound	Pendred,syndrome
R4570	T7458	T7459	amod	free,radicals
R4571	T7459	T7457	dobj	radicals,detoxifying
R4572	T7460	T7461	punct	(,Fig.
R4573	T7461	T7446	parataxis	Fig.,protect
R4574	T7462	T7461	nummod	9,Fig.
R4575	T7463	T7461	punct	),Fig.
R4576	T7464	T7446	punct	.,protect
R4577	T7466	T7467	nsubjpass	CO2,generated
R4578	T7468	T7466	cc	and,CO2
R4579	T7469	T7470	amod	free,radicals
R458	T909	T907	pobj	syndrome,of
R4580	T7470	T7466	conj	radicals,CO2
R4581	T7471	T7467	auxpass	are,generated
R4582	T7472	T7467	agent	by,generated
R4583	T7473	T7474	det	the,numbers
R4584	T7474	T7472	pobj	numbers,by
R4585	T7475	T7474	amod	large,numbers
R4586	T7476	T7474	prep	of,numbers
R4587	T7477	T7476	pobj	mitochondria,of
R4588	T7478	T7474	prep	in,numbers
R4589	T7479	T7480	det	the,cells
R459	T910	T911	punct	[,5
R4590	T7480	T7478	pobj	cells,in
R4591	T7481	T7482	advmod	metabolically,active
R4592	T7482	T7480	amod	active,cells
R4593	T7483	T7482	advmod	highly,active
R4594	T7484	T7485	amod	strial,marginal
R4595	T7485	T7480	amod	marginal,cells
R4596	T7486	T7467	punct	.,generated
R4597	T7488	T7489	amod	Intermediate,cells
R4598	T7489	T7490	nsubj	cells,employ
R4599	T7491	T7492	amod	carbonic,anhydrase
R46	T280	T279	prep	for,codes
R460	T911	T901	parataxis	5,be
R4600	T7492	T7490	dobj	anhydrase,employ
R4601	T7493	T7494	aux	to,convert
R4602	T7494	T7490	advcl	convert,employ
R4603	T7495	T7494	dobj	CO2,convert
R4604	T7496	T7494	prep	to,convert
R4605	T7497	T7496	pobj	HCO3,to
R4606	T7498	T7497	punct	-,HCO3
R4607	T7499	T7500	punct	[,19
R4608	T7500	T7494	parataxis	19,convert
R4609	T7501	T7500	nummod	18,19
R461	T912	T911	punct	],5
R4610	T7502	T7500	punct	",",19
R4611	T7503	T7500	punct	],19
R4612	T7504	T7490	cc	and,employ
R4613	T7505	T7490	conj	catalase,employ
R4614	T7506	T7507	aux	to,detoxify
R4615	T7507	T7505	advcl	detoxify,catalase
R4616	T7508	T7509	amod	free,radicals
R4617	T7509	T7507	dobj	radicals,detoxify
R4618	T7510	T7490	punct	.,employ
R4619	T7512	T7513	aux	To,protect
R462	T913	T894	punct	.,appear
R4620	T7513	T7514	advcl	protect,generate
R4621	T7515	T7513	dobj	themselves,protect
R4622	T7516	T7513	prep	from,protect
R4623	T7517	T7518	amod	free,damage
R4624	T7518	T7516	pobj	damage,from
R4625	T7519	T7518	compound	radical,damage
R4626	T7520	T7514	punct	", ",generate
R4627	T7521	T7522	amod	intermediate,cells
R4628	T7522	T7514	nsubj	cells,generate
R4629	T7523	T7514	dobj	glutathione,generate
R463	T915	T916	nsubj	Pendrin,is
R4630	T7524	T7523	cc	and,glutathione
R4631	T7525	T7526	compound	melanin,pigment
R4632	T7526	T7523	conj	pigment,glutathione
R4633	T7527	T7528	punct	[,34
R4634	T7528	T7514	parataxis	34,generate
R4635	T7529	T7530	punct	-,37
R4636	T7530	T7528	prep	37,34
R4637	T7531	T7528	punct	],34
R4638	T7532	T7514	punct	.,generate
R4639	T7534	T7535	nsubj	It,is
R464	T917	T918	det	an,exchanger
R4640	T7536	T7535	acomp	conceivable,is
R4641	T7537	T7538	mark	that,results
R4642	T7538	T7535	ccomp	results,is
R4643	T7539	T7538	nsubj	loss,results
R4644	T7540	T7539	prep	of,loss
R4645	T7541	T7540	pobj	pendrin,of
R4646	T7542	T7541	punct	", ",pendrin
R4647	T7543	T7544	dep	which,secrete
R4648	T7544	T7541	relcl	secrete,pendrin
R4649	T7545	T7544	aux	may,secrete
R465	T918	T916	attr	exchanger,is
R4650	T7546	T7544	dobj	HCO3,secrete
R4651	T7547	T7546	punct	-,HCO3
R4652	T7548	T7544	prep	into,secrete
R4653	T7549	T7548	pobj	endolymph,into
R4654	T7550	T7538	punct	", ",results
R4655	T7551	T7538	prep	in,results
R4656	T7552	T7553	det	an,accumulation
R4657	T7553	T7551	pobj	accumulation,in
R4658	T7554	T7553	prep	of,accumulation
R4659	T7555	T7554	pobj	HCO3,of
R466	T919	T918	compound	anion,exchanger
R4660	T7556	T7555	punct	-,HCO3
R4661	T7557	T7553	cc	and,accumulation
R4662	T7558	T7559	det	an,alkalinization
R4663	T7559	T7553	conj	alkalinization,accumulation
R4664	T7560	T7559	prep	of,alkalinization
R4665	T7561	T7562	det	the,spaces
R4666	T7562	T7560	pobj	spaces,of
R4667	T7563	T7562	amod	intrastrial,spaces
R4668	T7564	T7535	punct	.,is
R4669	T7566	T7567	det	This,alkalinization
R467	T920	T921	dep	that,transport
R4670	T7567	T7569	nsubj	alkalinization,enhance
R4671	T7568	T7567	amod	extracellular,alkalinization
R4672	T7570	T7569	aux	may,enhance
R4673	T7571	T7572	amod	free,radical
R4674	T7572	T7573	compound	radical,stress
R4675	T7573	T7569	dobj	stress,enhance
R4676	T7574	T7569	punct	", ",enhance
R4677	T7575	T7576	mark	since,inhibit
R4678	T7576	T7569	advcl	inhibit,enhance
R4679	T7577	T7576	nsubj	it,inhibit
R468	T921	T918	relcl	transport,exchanger
R4680	T7578	T7576	aux	may,inhibit
R4681	T7579	T7580	det	the,uptake
R4682	T7580	T7576	dobj	uptake,inhibit
R4683	T7581	T7580	prep	of,uptake
R4684	T7582	T7581	pobj	cysteine,of
R4685	T7583	T7576	cc	and,inhibit
R4686	T7584	T7585	advmod	thereby,limit
R4687	T7585	T7576	conj	limit,inhibit
R4688	T7586	T7585	dobj	production,limit
R4689	T7587	T7586	prep	of,production
R469	T922	T921	aux	can,transport
R4690	T7588	T7589	det	the,glutathione
R4691	T7589	T7587	pobj	glutathione,of
R4692	T7590	T7589	amod	protective,glutathione
R4693	T7591	T7592	punct	[,38
R4694	T7592	T7569	parataxis	38,enhance
R4695	T7593	T7592	punct	],38
R4696	T7594	T7569	punct	.,enhance
R4697	T7596	T7597	nsubj	Support,comes
R4698	T7598	T7596	prep	for,Support
R4699	T7599	T7600	det	the,hypothesis
R47	T281	T280	pobj	pendrin,for
R470	T923	T921	dobj	Cl,transport
R4700	T7600	T7598	pobj	hypothesis,for
R4701	T7601	T7600	prep	of,hypothesis
R4702	T7602	T7603	amod	enhanced,stress
R4703	T7603	T7601	pobj	stress,of
R4704	T7604	T7605	amod	free,radical
R4705	T7605	T7603	compound	radical,stress
R4706	T7606	T7597	prep	from,comes
R4707	T7607	T7608	det	the,hyperpigmentation
R4708	T7608	T7606	pobj	hyperpigmentation,from
R4709	T7609	T7608	amod	observed,hyperpigmentation
R471	T924	T923	punct	-,Cl
R4710	T7610	T7608	prep	in,hyperpigmentation
R4711	T7611	T7610	pobj	mice,in
R4712	T7612	T7611	acl	lacking,mice
R4713	T7613	T7612	dobj	pendrin,lacking
R4714	T7614	T7597	punct	.,comes
R4715	T7616	T7617	amod	Strial,hyperpigmentation
R4716	T7617	T7618	nsubjpass	hyperpigmentation,observed
R4717	T7619	T7618	aux	has,observed
R4718	T7620	T7618	advmod	also,observed
R4719	T7621	T7618	auxpass	been,observed
R472	T925	T923	punct	", ",Cl
R4720	T7622	T7618	prep	in,observed
R4721	T7623	T7624	amod	other,conditions
R4722	T7624	T7622	pobj	conditions,in
R4723	T7625	T7626	dep	that,associated
R4724	T7626	T7624	relcl	associated,conditions
R4725	T7627	T7626	auxpass	are,associated
R4726	T7628	T7626	prep	with,associated
R4727	T7629	T7630	amod	free,radical
R4728	T7630	T7631	compound	radical,stress
R4729	T7631	T7628	pobj	stress,with
R473	T926	T923	conj	I,Cl
R4730	T7632	T7624	punct	", ",conditions
R4731	T7633	T7634	amod	such,as
R4732	T7634	T7624	prep	as,conditions
R4733	T7635	T7636	amod	acoustic,trauma
R4734	T7636	T7634	pobj	trauma,as
R4735	T7637	T7638	punct	[,37
R4736	T7638	T7618	parataxis	37,observed
R4737	T7639	T7638	punct	],37
R4738	T7640	T7618	punct	.,observed
R4739	T7642	T7643	nsubj	Alterations,lead
R474	T927	T926	punct	-,I
R4740	T7644	T7642	prep	in,Alterations
R4741	T7645	T7646	det	the,pH
R4742	T7646	T7644	pobj	pH,in
R4743	T7647	T7646	amod	cytosolic,pH
R4744	T7648	T7642	prep	in,Alterations
R4745	T7649	T7648	pobj	conjunction,in
R4746	T7650	T7649	prep	with,conjunction
R4747	T7651	T7652	amod	free,radical
R4748	T7652	T7653	compound	radical,stress
R4749	T7653	T7650	pobj	stress,with
R475	T928	T926	punct	", ",I
R4750	T7654	T7643	aux	may,lead
R4751	T7655	T7643	prep	to,lead
R4752	T7656	T7657	det	the,loss
R4753	T7657	T7655	pobj	loss,to
R4754	T7658	T7657	prep	of,loss
R4755	T7659	T7660	compound	KCNJ10,expression
R4756	T7660	T7658	pobj	expression,of
R4757	T7661	T7660	compound	protein,expression
R4758	T7662	T7643	prep	in,lead
R4759	T7663	T7664	amod	strial,cells
R476	T929	T926	conj	HCO3,I
R4760	T7664	T7662	pobj	cells,in
R4761	T7665	T7664	amod	intermediate,cells
R4762	T7666	T7643	punct	.,lead
R4763	T7668	T7669	nsubjpass	Function,shown
R4764	T7670	T7668	cc	and,Function
R4765	T7671	T7668	conj	expression,Function
R4766	T7672	T7668	prep	of,Function
R4767	T7673	T7674	amod	other,channels
R4768	T7674	T7672	pobj	channels,of
R4769	T7675	T7674	nmod	K,channels
R477	T930	T929	punct	-,HCO3
R4770	T7676	T7675	punct	+,K
R4771	T7677	T7669	aux	has,shown
R4772	T7678	T7669	auxpass	been,shown
R4773	T7679	T7680	aux	to,controlled
R4774	T7680	T7669	xcomp	controlled,shown
R4775	T7681	T7680	auxpass	be,controlled
R4776	T7682	T7680	agent	by,controlled
R4777	T7683	T7684	det	the,pH
R4778	T7684	T7682	pobj	pH,by
R4779	T7685	T7684	amod	cytosolic,pH
R478	T931	T929	cc	and,HCO3
R4780	T7686	T7684	cc	and,pH
R4781	T7687	T7688	amod	free,radicals
R4782	T7688	T7684	conj	radicals,pH
R4783	T7689	T7684	punct	", ",pH
R4784	T7690	T7691	dep	which,encode
R4785	T7691	T7684	relcl	encode,pH
R4786	T7692	T7693	det	the,state
R4787	T7693	T7691	dobj	state,encode
R4788	T7694	T7693	amod	metabolic,state
R4789	T7695	T7693	prep	of,state
R479	T932	T929	conj	formate,HCO3
R4790	T7696	T7697	det	the,cell
R4791	T7697	T7695	pobj	cell,of
R4792	T7698	T7699	punct	[,39
R4793	T7699	T7669	parataxis	39,shown
R4794	T7700	T7699	punct	],39
R4795	T7701	T7669	punct	.,shown
R4796	T7703	T7704	nsubj	Suppression,is
R4797	T7705	T7703	prep	of,Suppression
R4798	T7706	T7707	det	the,channel
R4799	T7707	T7705	pobj	channel,of
R48	T282	T257	punct	.,caused
R480	T933	T934	punct	[,7
R4800	T7708	T7707	nmod	KCNJ10,channel
R4801	T7709	T7707	nmod	K,channel
R4802	T7710	T7709	punct	+,K
R4803	T7711	T7703	prep	in,Suppression
R4804	T7712	T7713	amod	strial,cells
R4805	T7713	T7711	pobj	cells,in
R4806	T7714	T7713	amod	intermediate,cells
R4807	T7715	T7703	punct	", ",Suppression
R4808	T7716	T7717	dep	which,is
R4809	T7717	T7703	relcl	is,Suppression
R481	T934	T921	parataxis	7,transport
R4810	T7718	T7717	acomp	essential,is
R4811	T7719	T7718	prep	for,essential
R4812	T7720	T7721	det	the,generation
R4813	T7721	T7719	pobj	generation,for
R4814	T7722	T7721	prep	of,generation
R4815	T7723	T7724	det	the,potential
R4816	T7724	T7722	pobj	potential,of
R4817	T7725	T7724	amod	endocochlear,potential
R4818	T7726	T7704	punct	", ",is
R4819	T7727	T7704	advmod	probably,is
R482	T935	T934	nummod	6,7
R4820	T7728	T7729	det	the,cause
R4821	T7729	T7704	attr	cause,is
R4822	T7730	T7729	amod	direct,cause
R4823	T7731	T7729	prep	of,cause
R4824	T7732	T7731	pobj	deafness,of
R4825	T7733	T7729	prep	in,cause
R4826	T7734	T7735	nmod	Slc26a4,mice
R4827	T7735	T7733	pobj	mice,in
R4828	T7736	T7734	punct	-,Slc26a4
R4829	T7737	T7734	punct	/,Slc26a4
R483	T936	T934	punct	",",7
R4830	T7738	T7734	punct	-,Slc26a4
R4831	T7739	T7735	cc	and,mice
R4832	T7740	T7735	conj	patients,mice
R4833	T7741	T7740	acl	suffering,patients
R4834	T7742	T7741	prep	from,suffering
R4835	T7743	T7744	compound	Pendred,syndrome
R4836	T7744	T7742	pobj	syndrome,from
R4837	T7745	T7704	punct	.,is
R4838	T7746	T7747	det	The,history
R4839	T7747	T7749	nsubjpass	history,accessed
R484	T937	T934	punct	],7
R4840	T7748	T7747	amod	pre-publication,history
R4841	T7750	T7747	prep	for,history
R4842	T7751	T7752	det	this,paper
R4843	T7752	T7750	pobj	paper,for
R4844	T7753	T7749	aux	can,accessed
R4845	T7754	T7749	auxpass	be,accessed
R4846	T7755	T7749	advmod	here,accessed
R4847	T7756	T7749	punct	:,accessed
R485	T938	T916	punct	.,is
R4850	T7896	T7897	compound	Protein,localization
R4851	T7898	T7897	prep	of,localization
R4852	T7899	T7898	pobj	pendrin,of
R4853	T7900	T7899	punct	", ",pendrin
R4854	T7901	T7899	conj	KCNQ1,pendrin
R4855	T7902	T7901	punct	", ",KCNQ1
R4856	T7903	T7901	conj	ZO,KCNQ1
R4857	T7904	T7903	punct	-,ZO
R4858	T7905	T7903	nummod	1,ZO
R4859	T7906	T7903	cc	and,ZO
R486	T940	T941	nsubjpass	Expression,found
R4860	T7907	T7908	compound	F,actin
R4861	T7908	T7903	conj	actin,ZO
R4862	T7909	T7897	prep	in,localization
R4863	T7910	T7909	pobj	cochlea,in
R4864	T7911	T7910	cc	and,cochlea
R4865	T7912	T7913	amod	vestibular,labyrinth
R4866	T7913	T7910	conj	labyrinth,cochlea
R4867	T7914	T7910	prep	of,cochlea
R4868	T7915	T7916	nmod	Slc26a4,mice
R4869	T7916	T7914	pobj	mice,of
R487	T942	T941	aux	has,found
R4870	T7917	T7915	punct	+,Slc26a4
R4871	T7918	T7915	punct	/,Slc26a4
R4872	T7919	T7915	punct	+,Slc26a4
R4873	T7920	T7915	cc	and,Slc26a4
R4874	T7921	T7915	conj	Slc26a4,Slc26a4
R4875	T7922	T7921	punct	-,Slc26a4
R4876	T7923	T7921	punct	/,Slc26a4
R4877	T7924	T7921	punct	-,Slc26a4
R4878	T7925	T7897	punct	.,localization
R4879	T7927	T7928	meta	a,μm
R488	T943	T941	auxpass	been,found
R4880	T7929	T7928	punct	: ,μm
R4881	T7930	T7928	dep	Overview,μm
R4882	T7931	T7930	prep	of,Overview
R4883	T7932	T7931	pobj	cochlea,of
R4884	T7933	T7928	punct	;,μm
R4885	T7934	T7928	nsubj	bar,μm
R4886	T7935	T7928	punct	=,μm
R4887	T7936	T7928	nummod	100,μm
R4888	T7937	T7928	punct	.,μm
R4889	T7939	T7940	meta	b,μm
R489	T944	T941	prep	in,found
R4890	T7941	T7939	punct	-,b
R4891	T7942	T7939	dep	f,b
R4892	T7943	T7940	punct	: ,μm
R4893	T7944	T7940	dep	Detail,μm
R4894	T7945	T7944	prep	of,Detail
R4895	T7946	T7947	amod	cochlear,wall
R4896	T7947	T7945	pobj	wall,of
R4897	T7948	T7947	amod	lateral,wall
R4898	T7949	T7940	punct	;,μm
R4899	T7950	T7940	nsubj	bar,μm
R49	T284	T285	nsubj	We,investigated
R490	T945	T946	det	the,ear
R4900	T7951	T7940	punct	=,μm
R4901	T7952	T7940	nummod	10,μm
R4902	T7953	T7940	punct	.,μm
R4903	T7955	T7956	meta	g,μm
R4904	T7957	T7956	punct	: ,μm
R4905	T7958	T7956	dep	Detail,μm
R4906	T7959	T7958	prep	of,Detail
R4907	T7960	T7959	pobj	utricle,of
R4908	T7961	T7956	punct	;,μm
R4909	T7962	T7956	nsubj	bar,μm
R491	T946	T944	pobj	ear,in
R4910	T7963	T7956	punct	=,μm
R4911	T7964	T7956	nummod	10,μm
R4912	T7965	T7956	punct	.,μm
R4913	T7967	T7968	meta	h,μm
R4914	T7969	T7967	punct	-,h
R4915	T7970	T7967	dep	i,h
R4916	T7971	T7968	punct	: ,μm
R4917	T7972	T7968	dep	Detail,μm
R4918	T7973	T7972	prep	of,Detail
R4919	T7974	T7973	pobj	ampulla,of
R492	T947	T946	amod	inner,ear
R4920	T7975	T7968	punct	;,μm
R4921	T7976	T7968	nsubj	bar,μm
R4922	T7977	T7968	punct	=,μm
R4923	T7978	T7968	nummod	10,μm
R4924	T7979	T7968	punct	.,μm
R4925	T7982	T7981	punct	", ",RM
R4926	T7983	T7984	poss	Reissner,membrane
R4927	T7984	T7981	appos	membrane,RM
R4928	T7985	T7983	case	's,Reissner
R4929	T7986	T7981	punct	;,RM
R493	T948	T946	cc	and,ear
R4930	T7987	T7981	appos	SC,RM
R4931	T7988	T7987	punct	", ",SC
R4932	T7989	T7990	npadvmod	spindle,shaped
R4933	T7990	T7992	amod	shaped,cells
R4934	T7991	T7990	punct	-,shaped
R4935	T7992	T7987	appos	cells,SC
R4936	T7993	T7981	punct	", ",RM
R4937	T7994	T7981	appos	SMC,RM
R4938	T7995	T7994	punct	", ",SMC
R4939	T7996	T7997	amod	strial,marginal
R494	T949	T950	compound	thyroid,gland
R4940	T7997	T7998	amod	marginal,cells
R4941	T7998	T7994	appos	cells,SMC
R4942	T7999	T7981	punct	;,RM
R4943	T8000	T7981	appos	SV,RM
R4944	T8001	T8000	punct	", ",SV
R4945	T8002	T8003	compound	stria,vascularis
R4946	T8003	T8000	appos	vascularis,SV
R4947	T8004	T7981	punct	;,RM
R4948	T8005	T7981	appos	SL,RM
R4949	T8006	T8005	punct	", ",SL
R495	T950	T946	conj	gland,ear
R4950	T8007	T8008	amod	spiral,ligament
R4951	T8008	T8005	appos	ligament,SL
R4952	T8009	T7981	punct	;,RM
R4953	T8010	T7981	appos	LIM,RM
R4954	T8011	T8010	punct	", ",LIM
R4955	T8012	T8013	amod	spiral,limbus
R4956	T8013	T8010	appos	limbus,LIM
R4957	T8014	T7981	punct	.,RM
R4958	T8017	T8016	punct	", ",BC
R4959	T8018	T8019	amod	basal,cells
R496	T951	T941	advcl	consistent,found
R4960	T8019	T8016	appos	cells,BC
R4961	T8020	T8016	punct	;,BC
R4962	T8021	T8016	appos	SP,BC
R4963	T8022	T8021	punct	", ",SP
R4964	T8023	T8024	amod	spiral,prominence
R4965	T8024	T8025	nmod	prominence,cells
R4966	T8025	T8021	appos	cells,SP
R4967	T8026	T8025	amod	epithelial,cells
R4968	T8027	T8016	punct	;,BC
R4969	T8028	T8016	appos	RC,BC
R497	T952	T951	prep	with,consistent
R4970	T8029	T8028	punct	", ",RC
R4971	T8030	T8031	compound	root,cells
R4972	T8031	T8028	appos	cells,RC
R4973	T8032	T8016	punct	;,BC
R4974	T8033	T8016	appos	OS,BC
R4975	T8034	T8033	punct	", ",OS
R4976	T8035	T8036	amod	outer,sulcus
R4977	T8036	T8037	nmod	sulcus,cells
R4978	T8037	T8033	appos	cells,OS
R4979	T8038	T8037	amod	epithelial,cells
R498	T953	T954	det	the,signs
R4980	T8039	T8016	punct	;,BC
R4981	T8040	T8016	appos	VHC,BC
R4982	T8041	T8040	punct	", ",VHC
R4983	T8042	T8043	amod	vestibular,cells
R4984	T8043	T8040	appos	cells,VHC
R4985	T8044	T8043	compound	hair,cells
R4986	T8045	T8016	punct	;,BC
R4987	T8046	T8016	appos	VTC,BC
R4988	T8047	T8046	punct	", ",VTC
R4989	T8048	T8049	amod	vestibular,cells
R499	T954	T952	pobj	signs,with
R4990	T8049	T8046	appos	cells,VTC
R4991	T8050	T8049	amod	transitional,cells
R4992	T8051	T8016	punct	;,BC
R4993	T8052	T8016	appos	VDC,BC
R4994	T8053	T8052	punct	", ",VDC
R4995	T8054	T8055	amod	vestibular,cells
R4996	T8055	T8052	appos	cells,VDC
R4997	T8056	T8055	amod	dark,cells
R4998	T8057	T8016	punct	;,BC
R4999	T8058	T8059	dep	arrows,form
R50	T286	T287	det	the,relationship
R500	T955	T954	amod	clinical,signs
R5000	T8059	T8016	dep	form,BC
R5001	T8060	T8059	punct	", ",form
R5002	T8061	T8062	amod	basal,cells
R5003	T8062	T8059	nsubj	cells,form
R5004	T8063	T8062	prep	at,cells
R5005	T8064	T8065	det	the,top
R5006	T8065	T8063	pobj	top,at
R5007	T8066	T8065	cc	and,top
R5008	T8067	T8065	conj	bottom,top
R5009	T8068	T8065	prep	of,top
R501	T956	T954	prep	of,signs
R5010	T8069	T8070	compound	stria,vascularis
R5011	T8070	T8068	pobj	vascularis,of
R5012	T8071	T8072	amod	tight,junctions
R5013	T8072	T8059	dobj	junctions,form
R5014	T8073	T8072	prep	with,junctions
R5015	T8074	T8075	nmod	surface,cells
R5016	T8075	T8073	pobj	cells,with
R5017	T8076	T8075	amod	epithelial,cells
R5018	T8077	T8016	punct	.,BC
R502	T957	T956	pobj	deafness,of
R503	T958	T957	cc	and,deafness
R5033	T8160	T8159	punct	", ",Potential
R5034	T8161	T8162	nmod	K,concentrations
R5035	T8162	T8159	conj	concentrations,Potential
R5036	T8163	T8161	punct	+,K
R5037	T8164	T8162	cc	and,concentrations
R5038	T8165	T8162	conj	pigmentation,concentrations
R5039	T8166	T8165	prep	of,pigmentation
R504	T959	T957	conj	goiter,deafness
R5040	T8167	T8168	compound	stria,vascularis
R5041	T8168	T8166	pobj	vascularis,of
R5042	T8169	T8159	prep	in,Potential
R5043	T8170	T8171	nmod	Slc26a4,mice
R5044	T8171	T8169	pobj	mice,in
R5045	T8172	T8170	punct	+,Slc26a4
R5046	T8173	T8170	punct	/,Slc26a4
R5047	T8174	T8170	punct	+,Slc26a4
R5048	T8175	T8170	cc	and,Slc26a4
R5049	T8176	T8170	conj	Slc26a4,Slc26a4
R505	T960	T961	punct	[,8
R5050	T8177	T8176	punct	-,Slc26a4
R5051	T8178	T8176	punct	/,Slc26a4
R5052	T8179	T8176	punct	-,Slc26a4
R5053	T8180	T8159	punct	.,Potential
R5054	T8182	T8183	meta	a,potential
R5055	T8184	T8183	punct	: ,potential
R5056	T8185	T8183	amod	Endocochlear,potential
R5057	T8186	T8183	cc	and,potential
R5058	T8187	T8188	nmod	K,concentrations
R5059	T8188	T8183	conj	concentrations,potential
R506	T961	T941	parataxis	8,found
R5060	T8189	T8187	punct	+,K
R5061	T8190	T8183	prep	in,potential
R5062	T8191	T8190	pobj	endolymph,in
R5063	T8192	T8191	cc	and,endolymph
R5064	T8193	T8191	conj	perilymph,endolymph
R5065	T8194	T8183	prep	at,potential
R5066	T8195	T8196	det	the,apex
R5067	T8196	T8194	pobj	apex,at
R5068	T8197	T8198	punct	(,A
R5069	T8198	T8196	parataxis	A,apex
R507	T962	T961	nummod	2,8
R5070	T8199	T8198	punct	),A
R5071	T8200	T8196	cc	and,apex
R5072	T8201	T8196	conj	base,apex
R5073	T8202	T8203	punct	(,B
R5074	T8203	T8201	parataxis	B,base
R5075	T8204	T8203	punct	),B
R5076	T8205	T8196	prep	of,apex
R5077	T8206	T8207	det	the,cochlea
R5078	T8207	T8205	pobj	cochlea,of
R5079	T8208	T8183	punct	.,potential
R508	T963	T961	punct	",",8
R5080	T8210	T8211	nsubj	Numbers,denote
R5081	T8212	T8210	amod	adjacent,Numbers
R5082	T8213	T8212	prep	to,adjacent
R5083	T8214	T8213	pobj	symbols,to
R5084	T8215	T8211	dobj	number,denote
R5085	T8216	T8215	prep	of,number
R5086	T8217	T8216	pobj	measurements,of
R5087	T8218	T8211	punct	.,denote
R5088	T8220	T8221	meta	b,Pigmentation
R5089	T8222	T8220	punct	-,b
R509	T964	T961	nummod	3,8
R5090	T8223	T8220	dep	d,b
R5091	T8224	T8221	punct	: ,Pigmentation
R5092	T8225	T8221	prep	of,Pigmentation
R5093	T8226	T8227	compound	stria,vascularis
R5094	T8227	T8225	pobj	vascularis,of
R5095	T8228	T8221	prep	in,Pigmentation
R5096	T8229	T8230	nmod	Slc26a4,mice
R5097	T8230	T8228	pobj	mice,in
R5098	T8231	T8229	punct	+,Slc26a4
R5099	T8232	T8229	punct	/,Slc26a4
R51	T287	T285	dobj	relationship,investigated
R510	T965	T961	punct	",",8
R5100	T8233	T8229	punct	+,Slc26a4
R5101	T8234	T8229	cc	and,Slc26a4
R5102	T8235	T8229	conj	Slc26a4,Slc26a4
R5103	T8236	T8235	punct	-,Slc26a4
R5104	T8237	T8235	punct	/,Slc26a4
R5105	T8238	T8235	punct	-,Slc26a4
R5106	T8239	T8221	punct	.,Pigmentation
R5107	T8241	T8242	meta	b,View
R5108	T8243	T8242	punct	: ,View
R5109	T8244	T8242	prep	of,View
R511	T966	T961	punct	],8
R5110	T8245	T8246	compound	stria,vascularis
R5111	T8246	T8244	pobj	vascularis,of
R5112	T8247	T8242	prep	through,View
R5113	T8248	T8249	det	the,capsule
R5114	T8249	T8247	pobj	capsule,through
R5115	T8250	T8249	amod	bony,capsule
R5116	T8251	T8249	prep	of,capsule
R5117	T8252	T8253	det	the,cochlea
R5118	T8253	T8251	pobj	cochlea,of
R5119	T8254	T8242	punct	.,View
R512	T967	T941	punct	.,found
R5120	T8257	T8256	punct	", ",OW
R5121	T8258	T8259	amod	oval,window
R5122	T8259	T8256	appos	window,OW
R5123	T8260	T8256	punct	", ",OW
R5124	T8261	T8256	appos	RW,OW
R5125	T8262	T8261	punct	", ",RW
R5126	T8263	T8264	amod	round,window
R5127	T8264	T8261	appos	window,RW
R5128	T8265	T8256	punct	;,OW
R5129	T8266	T8256	appos	arrows,OW
R513	T969	T970	prep	In,found
R5130	T8267	T8266	punct	", ",arrows
R5131	T8268	T8269	compound	stria,vascularis
R5132	T8269	T8266	appos	vascularis,arrows
R5133	T8270	T8256	punct	.,OW
R5134	T8272	T8273	meta	c,mounts
R5135	T8274	T8272	punct	-,c
R5136	T8275	T8272	dep	d,c
R5137	T8276	T8273	punct	: ,mounts
R5138	T8277	T8273	amod	Whole,mounts
R5139	T8278	T8273	punct	-,mounts
R514	T971	T969	pobj	addition,In
R5140	T8279	T8273	prep	of,mounts
R5141	T8280	T8281	compound	stria,vascularis
R5142	T8281	T8279	pobj	vascularis,of
R5143	T8282	T8273	acl	isolated,mounts
R5144	T8283	T8282	prep	from,isolated
R5145	T8284	T8285	npadvmod	age,matched
R5146	T8285	T8287	amod	matched,mice
R5147	T8286	T8285	punct	-,matched
R5148	T8287	T8283	pobj	mice,from
R5149	T8288	T8273	punct	.,mounts
R515	T972	T970	punct	", ",found
R5150	T8290	T8291	meta	c,μm
R5151	T8291	T8301	dep	μm,Quantification
R5152	T8292	T8291	punct	: ,μm
R5153	T8293	T8294	nmod	Laser,images
R5154	T8294	T8291	dep	images,μm
R5155	T8295	T8293	punct	-,Laser
R5156	T8296	T8293	amod	scanning,Laser
R5157	T8297	T8291	punct	", ",μm
R5158	T8298	T8291	nsubj	bar,μm
R5159	T8299	T8291	punct	=,μm
R516	T973	T974	compound	pendrin,expression
R5160	T8300	T8291	nummod	10,μm
R5161	T8302	T8301	punct	", ",Quantification
R5162	T8303	T8301	meta	d,Quantification
R5163	T8304	T8301	punct	: ,Quantification
R5164	T8305	T8301	prep	of,Quantification
R5165	T8306	T8305	pobj	pigmentation,of
R5166	T8307	T8301	prep	based,Quantification
R5167	T8308	T8307	prep	on,based
R5168	T8309	T8310	amod	optical,density
R5169	T8310	T8308	pobj	density,on
R517	T974	T970	nsubjpass	expression,found
R5170	T8311	T8301	punct	.,Quantification
R5173	T8436	T8435	prep	of,Quantification
R5174	T8437	T8438	nmod	KCNJ10,expression
R5175	T8438	T8436	pobj	expression,of
R5176	T8439	T8437	cc	and,KCNJ10
R5177	T8440	T8437	conj	KCNQ1,KCNJ10
R5178	T8441	T8438	compound	mRNA,expression
R5179	T8442	T8438	prep	in,expression
R518	T975	T970	aux	has,found
R5180	T8443	T8444	compound	stria,vascularis
R5181	T8444	T8442	pobj	vascularis,in
R5182	T8445	T8444	cc	and,vascularis
R5183	T8446	T8447	amod	spiral,ganglia
R5184	T8447	T8444	conj	ganglia,vascularis
R5185	T8448	T8444	prep	of,vascularis
R5186	T8449	T8450	nmod	Slc26a4,mice
R5187	T8450	T8448	pobj	mice,of
R5188	T8451	T8449	punct	+,Slc26a4
R5189	T8452	T8449	punct	/,Slc26a4
R519	T976	T970	auxpass	been,found
R5190	T8453	T8449	punct	+,Slc26a4
R5191	T8454	T8449	cc	and,Slc26a4
R5192	T8455	T8449	conj	Slc26a4,Slc26a4
R5193	T8456	T8455	punct	-,Slc26a4
R5194	T8457	T8455	punct	/,Slc26a4
R5195	T8458	T8455	punct	-,Slc26a4
R5196	T8459	T8435	punct	.,Quantification
R5197	T8461	T8462	meta	a,Electropherogram
R5198	T8463	T8462	punct	: ,Electropherogram
R5199	T8464	T8462	prep	of,Electropherogram
R52	T288	T287	prep	between,relationship
R520	T977	T970	prep	in,found
R5200	T8465	T8466	amod	total,RNA
R5201	T8466	T8464	pobj	RNA,of
R5202	T8467	T8466	acl	isolated,RNA
R5203	T8468	T8467	prep	from,isolated
R5204	T8469	T8470	compound	stria,vascularis
R5205	T8470	T8468	pobj	vascularis,from
R5206	T8471	T8470	acl	microdissected,vascularis
R5207	T8472	T8471	prep	from,microdissected
R5208	T8473	T8474	nummod	one,mouse
R5209	T8474	T8472	pobj	mouse,from
R521	T978	T979	det	the,kidney
R5210	T8475	T8462	punct	.,Electropherogram
R5211	T8477	T8478	det	The,amount
R5212	T8478	T8479	nsubjpass	amount,obtained
R5213	T8480	T8478	prep	of,amount
R5214	T8481	T8482	amod	total,RNA
R5215	T8482	T8480	pobj	RNA,of
R5216	T8483	T8479	auxpass	was,obtained
R5217	T8484	T8479	prep	from,obtained
R5218	T8485	T8486	det	the,integral
R5219	T8486	T8484	pobj	integral,from
R522	T979	T977	pobj	kidney,in
R5220	T8487	T8486	amod	total,integral
R5221	T8488	T8489	punct	(,shaded
R5222	T8489	T8486	parataxis	shaded,integral
R5223	T8490	T8489	punct	),shaded
R5224	T8491	T8479	cc	and,obtained
R5225	T8492	T8493	det	the,amount
R5226	T8493	T8494	nsubjpass	amount,obtained
R5227	T8494	T8479	conj	obtained,obtained
R5228	T8495	T8493	prep	of,amount
R5229	T8496	T8497	compound	18S,rRNA
R523	T980	T981	punct	[,9
R5230	T8497	T8495	pobj	rRNA,of
R5231	T8498	T8494	auxpass	was,obtained
R5232	T8499	T8494	prep	from,obtained
R5233	T8500	T8501	det	the,integral
R5234	T8501	T8499	pobj	integral,from
R5235	T8502	T8501	prep	of,integral
R5236	T8503	T8504	det	the,peak
R5237	T8504	T8502	pobj	peak,of
R5238	T8505	T8504	compound	18S,peak
R5239	T8506	T8494	punct	.,obtained
R524	T981	T979	parataxis	9,kidney
R5240	T8508	T8509	amod	Sharp,peaks
R5241	T8509	T8510	nsubj	peaks,demonstrate
R5242	T8511	T8509	acl	representing,peaks
R5243	T8512	T8513	nmod	18S,rRNA
R5244	T8513	T8511	dobj	rRNA,representing
R5245	T8514	T8512	cc	and,18S
R5246	T8515	T8512	conj	28S,18S
R5247	T8516	T8517	det	the,quality
R5248	T8517	T8510	dobj	quality,demonstrate
R5249	T8518	T8517	amod	high,quality
R525	T982	T981	punct	],9
R5250	T8519	T8517	prep	of,quality
R5251	T8520	T8519	pobj	RNA,of
R5252	T8521	T8510	punct	.,demonstrate
R5253	T8523	T8524	dep	Insert,verified
R5254	T8525	T8524	punct	: ,verified
R5255	T8526	T8524	nsubjpass	Genotype,verified
R5256	T8527	T8526	prep	of,Genotype
R5257	T8528	T8529	nmod	Slc26a4,mice
R5258	T8529	T8527	pobj	mice,of
R5259	T8530	T8528	punct	-,Slc26a4
R526	T983	T979	punct	", ",kidney
R5260	T8531	T8528	punct	/,Slc26a4
R5261	T8532	T8528	punct	-,Slc26a4
R5262	T8533	T8524	auxpass	was,verified
R5263	T8534	T8524	prep	by,verified
R5264	T8535	T8536	det	the,observation
R5265	T8536	T8534	pobj	observation,by
R5266	T8537	T8536	prep	of,observation
R5267	T8538	T8539	nummod	one,otoconia
R5268	T8539	T8537	pobj	otoconia,of
R5269	T8540	T8538	cc	or,one
R527	T984	T985	amod	mammary,gland
R5270	T8541	T8538	conj	few,one
R5271	T8542	T8543	advmod	very,large
R5272	T8543	T8539	amod	large,otoconia
R5273	T8544	T8539	amod	rhomboedric,otoconia
R5274	T8545	T8539	prep	in,otoconia
R5275	T8546	T8547	det	the,macula
R5276	T8547	T8545	pobj	macula,in
R5277	T8548	T8547	amod	utricular,macula
R5278	T8549	T8550	punct	(,arrow
R5279	T8550	T8524	parataxis	arrow,verified
R528	T985	T979	conj	gland,kidney
R5280	T8551	T8550	punct	),arrow
R5281	T8552	T8524	punct	.,verified
R5282	T8555	T8554	punct	", ",A
R5283	T8556	T8557	compound	crista,ampullaris
R5284	T8557	T8554	appos	ampullaris,A
R5285	T8558	T8554	punct	;,A
R5286	T8559	T8554	appos	U,A
R5287	T8560	T8559	punct	", ",U
R5288	T8561	T8562	amod	utricular,macula
R5289	T8562	T8559	appos	macula,U
R529	T986	T987	punct	[,10
R5290	T8563	T8554	punct	.,A
R5291	T8565	T8566	compound	Scale,bar
R5292	T8566	T8567	dep	bar,μm
R5293	T8568	T8567	punct	: ,μm
R5294	T8569	T8567	nummod	100,μm
R5295	T8570	T8567	punct	.,μm
R5296	T8572	T8573	meta	b,Example
R5297	T8574	T8573	punct	: ,Example
R5298	T8575	T8573	prep	of,Example
R5299	T8576	T8577	amod	real,time
R53	T289	T288	pobj	pendrin,between
R530	T987	T985	parataxis	10,gland
R5300	T8577	T8579	compound	time,data
R5301	T8578	T8577	punct	-,time
R5302	T8579	T8575	pobj	data,of
R5303	T8580	T8581	compound	RT,PCR
R5304	T8581	T8579	compound	PCR,data
R5305	T8582	T8581	punct	-,PCR
R5306	T8583	T8579	acl	used,data
R5307	T8584	T8583	prep	for,used
R5308	T8585	T8584	pobj	quantification,for
R5309	T8586	T8585	prep	of,quantification
R531	T988	T987	punct	],10
R5310	T8587	T8586	pobj	18S,of
R5311	T8588	T8587	punct	", ",18S
R5312	T8589	T8587	conj	KCNJ10,18S
R5313	T8590	T8589	punct	", ",KCNJ10
R5314	T8591	T8589	conj	KCNQ1,KCNJ10
R5315	T8592	T8591	cc	and,KCNQ1
R5316	T8593	T8591	conj	KCNQ4,KCNQ1
R5317	T8594	T8573	punct	.,Example
R5318	T8596	T8597	amod	Known,quantities
R5319	T8597	T8598	nsubjpass	quantities,used
R532	T989	T985	punct	", ",gland
R5320	T8599	T8597	prep	of,quantities
R5321	T8600	T8601	compound	18S,rRNA
R5322	T8601	T8599	pobj	rRNA,of
R5323	T8602	T8598	auxpass	were,used
R5324	T8603	T8604	aux	to,calibrate
R5325	T8604	T8598	advcl	calibrate,used
R5326	T8605	T8606	det	the,threshold
R5327	T8606	T8604	dobj	threshold,calibrate
R5328	T8607	T8598	punct	.,used
R5329	T8610	T8609	punct	", ",SV
R533	T990	T985	conj	uterus,gland
R5330	T8611	T8612	compound	stria,vascularis
R5331	T8612	T8609	appos	vascularis,SV
R5332	T8613	T8609	punct	;,SV
R5333	T8614	T8609	appos	SG,SV
R5334	T8615	T8614	punct	", ",SG
R5335	T8616	T8617	amod	spiral,ganglia
R5336	T8617	T8614	appos	ganglia,SG
R5337	T8618	T8609	punct	.,SV
R5338	T8620	T8621	meta	c,Quantification
R5339	T8622	T8621	punct	: ,Quantification
R534	T991	T992	punct	[,11
R5340	T8623	T8621	prep	of,Quantification
R5341	T8624	T8625	nmod	KCNJ10,mRNA
R5342	T8625	T8623	pobj	mRNA,of
R5343	T8626	T8624	cc	and,KCNJ10
R5344	T8627	T8624	conj	KCNQ1,KCNJ10
R5345	T8628	T8621	prep	in,Quantification
R5346	T8629	T8630	amod	young,Slc26a4
R5347	T8630	T8628	pobj	Slc26a4,in
R5348	T8631	T8630	punct	+,Slc26a4
R5349	T8632	T8630	punct	/,Slc26a4
R535	T992	T990	parataxis	11,uterus
R5350	T8633	T8630	punct	+,Slc26a4
R5351	T8634	T8630	cc	and,Slc26a4
R5352	T8635	T8636	amod	young,-
R5353	T8636	T8642	punct	-,mice
R5354	T8637	T8635	cc	and,young
R5355	T8638	T8635	conj	old,young
R5356	T8639	T8636	nmod	Slc26a4,-
R5357	T8640	T8636	punct	-,-
R5358	T8641	T8636	punct	/,-
R5359	T8642	T8630	conj	mice,Slc26a4
R536	T993	T992	punct	],11
R5360	T8643	T8621	punct	.,Quantification
R5361	T8717	T8718	compound	Protein,localization
R5362	T8719	T8718	prep	of,localization
R5363	T8720	T8719	pobj	KCNJ10,of
R5364	T8721	T8718	prep	in,localization
R5365	T8722	T8723	det	the,cochlea
R5366	T8723	T8721	pobj	cochlea,in
R5367	T8724	T8723	prep	of,cochlea
R5368	T8725	T8726	nmod	Slc26a4,mice
R5369	T8726	T8724	pobj	mice,of
R537	T994	T990	punct	", ",uterus
R5370	T8727	T8725	punct	+,Slc26a4
R5371	T8728	T8725	punct	/,Slc26a4
R5372	T8729	T8725	punct	+,Slc26a4
R5373	T8730	T8725	cc	and,Slc26a4
R5374	T8731	T8725	conj	Slc26a4,Slc26a4
R5375	T8732	T8731	punct	-,Slc26a4
R5376	T8733	T8731	punct	/,Slc26a4
R5377	T8734	T8731	punct	-,Slc26a4
R5378	T8735	T8718	punct	.,localization
R5379	T8737	T8738	meta	a,μm
R538	T995	T990	conj	testes,uterus
R5380	T8739	T8738	punct	: ,μm
R5381	T8740	T8738	dep	Overview,μm
R5382	T8741	T8740	prep	of,Overview
R5383	T8742	T8741	pobj	cochlea,of
R5384	T8743	T8738	punct	;,μm
R5385	T8744	T8738	nsubj	bar,μm
R5386	T8745	T8738	punct	=,μm
R5387	T8746	T8738	nummod	100,μm
R5388	T8747	T8738	punct	.,μm
R5389	T8750	T8749	prep	to,Compare
R539	T996	T997	punct	[,12
R5390	T8751	T8750	pobj	Fig.,to
R5391	T8752	T8751	nummod	1a,Fig.
R5392	T8753	T8754	aux	to,note
R5393	T8754	T8749	advcl	note,Compare
R5394	T8755	T8756	det	the,media
R5395	T8756	T8754	dobj	media,note
R5396	T8757	T8756	amod	enlarged,media
R5397	T8758	T8756	compound	scala,media
R5398	T8759	T8756	cc	and,media
R5399	T8760	T8761	det	the,membrane
R54	T290	T289	cc	and,pendrin
R540	T997	T995	parataxis	12,testes
R5400	T8761	T8756	conj	membrane,media
R5401	T8762	T8761	amod	distended,membrane
R5402	T8763	T8761	poss	Reissner,membrane
R5403	T8764	T8763	case	's,Reissner
R5404	T8765	T8749	punct	.,Compare
R5405	T8767	T8768	meta	b,Detail
R5406	T8769	T8767	punct	-,b
R5407	T8770	T8767	dep	c,b
R5408	T8771	T8768	punct	: ,Detail
R5409	T8772	T8768	prep	of,Detail
R541	T998	T997	punct	],12
R5410	T8773	T8774	amod	lateral,wall
R5411	T8774	T8772	pobj	wall,of
R5412	T8775	T8774	cc	and,wall
R5413	T8776	T8777	amod	spiral,ganglia
R5414	T8777	T8774	conj	ganglia,wall
R5415	T8778	T8779	punct	(,insert
R5416	T8779	T8768	parataxis	insert,Detail
R5417	T8780	T8779	punct	),insert
R5418	T8781	T8768	punct	;,Detail
R5419	T8782	T8783	amod	main,bar
R542	T999	T995	cc	and,testes
R5420	T8783	T8784	nsubj	bar,μm
R5421	T8784	T8768	dep	μm,Detail
R5422	T8785	T8784	punct	: ,μm
R5423	T8786	T8784	nummod	10,μm
R5424	T8787	T8768	punct	", ",Detail
R5425	T8788	T8789	nsubj	insert,μm
R5426	T8789	T8768	dep	μm,Detail
R5427	T8790	T8789	punct	: ,μm
R5428	T8791	T8789	nummod	5,μm
R5429	T8792	T8768	punct	.,Detail
R543	T1000	T995	conj	placenta,testes
R5430	T8794	T8795	nsubj	Expression,was
R5431	T8796	T8794	prep	of,Expression
R5432	T8797	T8796	pobj	KCNJ10,of
R5433	T8798	T8794	prep	in,Expression
R5434	T8799	T8800	nmod	Slc26a4,mice
R5435	T8800	T8798	pobj	mice,in
R5436	T8801	T8799	punct	-,Slc26a4
R5437	T8802	T8799	punct	/,Slc26a4
R5438	T8803	T8799	punct	-,Slc26a4
R5439	T8804	T8795	acomp	absent,was
R544	T1001	T1002	punct	[,13
R5440	T8805	T8804	prep	in,absent
R5441	T8806	T8807	compound	stria,vascularis
R5442	T8807	T8805	pobj	vascularis,in
R5443	T8808	T8804	cc	but,absent
R5444	T8809	T8804	conj	unchanged,absent
R5445	T8810	T8809	prep	in,unchanged
R5446	T8811	T8812	amod	spiral,ganglion
R5447	T8812	T8813	compound	ganglion,cells
R5448	T8813	T8810	pobj	cells,in
R5449	T8814	T8795	punct	.,was
R545	T1002	T1000	parataxis	13,placenta
R5450	T8817	T8816	punct	", ",RM
R5451	T8818	T8819	poss	Reissner,membrane
R5452	T8819	T8816	appos	membrane,RM
R5453	T8820	T8818	case	's,Reissner
R5454	T8821	T8816	punct	", ",RM
R5455	T8822	T8816	appos	SV,RM
R5456	T8823	T8822	punct	", ",SV
R5457	T8824	T8825	compound	stria,vascularis
R5458	T8825	T8822	appos	vascularis,SV
R5459	T8826	T8816	punct	;,RM
R546	T1003	T1002	punct	],13
R5460	T8827	T8816	appos	SP,RM
R5461	T8828	T8827	punct	", ",SP
R5462	T8829	T8830	amod	spiral,prominence
R5463	T8830	T8827	appos	prominence,SP
R5464	T8831	T8816	punct	;,RM
R5465	T8832	T8816	appos	SL,RM
R5466	T8833	T8832	punct	", ",SL
R5467	T8834	T8835	amod	spiral,ligament
R5468	T8835	T8832	appos	ligament,SL
R5469	T8836	T8816	punct	;,RM
R547	T1004	T970	punct	.,found
R5470	T8837	T8816	appos	LIM,RM
R5471	T8838	T8837	punct	", ",LIM
R5472	T8839	T8840	amod	spiral,limbus
R5473	T8840	T8837	appos	limbus,LIM
R5474	T8841	T8816	punct	;,RM
R5475	T8842	T8816	appos	SG,RM
R5476	T8843	T8842	punct	", ",SG
R5477	T8844	T8845	amod	spiral,ganglion
R5478	T8845	T8842	appos	ganglion,SG
R5479	T8846	T8816	punct	.,RM
R548	T1006	T1007	det	No,expression
R5484	T8911	T8912	amod	Morphometric,analysis
R5485	T8913	T8912	prep	of,analysis
R5486	T8914	T8915	amod	cochlear,masses
R5487	T8915	T8913	pobj	masses,of
R5488	T8916	T8915	compound	tissue,masses
R5489	T8917	T8912	prep	in,analysis
R549	T1007	T1008	nsubjpass	expression,found
R5490	T8918	T8919	nmod	Slc26a4,mice
R5491	T8919	T8917	pobj	mice,in
R5492	T8920	T8918	punct	+,Slc26a4
R5493	T8921	T8918	punct	/,Slc26a4
R5494	T8922	T8918	punct	+,Slc26a4
R5495	T8923	T8918	cc	and,Slc26a4
R5496	T8924	T8918	conj	Slc26a4,Slc26a4
R5497	T8925	T8924	punct	-,Slc26a4
R5498	T8926	T8924	punct	/,Slc26a4
R5499	T8927	T8924	punct	-,Slc26a4
R55	T291	T289	conj	deafness,pendrin
R550	T1009	T1008	auxpass	was,found
R5500	T8928	T8912	punct	.,analysis
R5501	T8930	T8931	meta	a,locations
R5502	T8932	T8931	punct	: ,locations
R5503	T8933	T8931	prep	of,locations
R5504	T8934	T8933	pobj	measurements,of
R5505	T8935	T8931	punct	.,locations
R5506	T8937	T8938	nsubjpass	Thickness,obtained
R5507	T8939	T8937	prep	of,Thickness
R5508	T8940	T8941	compound	stria,vascularis
R5509	T8941	T8939	pobj	vascularis,of
R551	T1010	T1008	prep	in,found
R5510	T8942	T8941	punct	(,vascularis
R5511	T8943	T8941	appos	SV,vascularis
R5512	T8944	T8941	punct	),vascularis
R5513	T8945	T8938	auxpass	was,obtained
R5514	T8946	T8938	prep	as,obtained
R5515	T8947	T8946	pobj	average,as
R5516	T8948	T8947	prep	of,average
R5517	T8949	T8950	nummod	three,measurements
R5518	T8950	T8948	pobj	measurements,of
R5519	T8951	T8950	compound	distance,measurements
R552	T1011	T1012	amod	fetal,brain
R5520	T8952	T8950	amod	perpendicular,measurements
R5521	T8953	T8952	prep	to,perpendicular
R5522	T8954	T8955	det	the,surface
R5523	T8955	T8953	pobj	surface,to
R5524	T8956	T8955	prep	of,surface
R5525	T8957	T8958	amod	marginal,cells
R5526	T8958	T8956	pobj	cells,of
R5527	T8959	T8938	punct	.,obtained
R5528	T8961	T8962	nsubjpass	Thickness,measured
R5529	T8963	T8961	prep	of,Thickness
R553	T1012	T1010	pobj	brain,in
R5530	T8964	T8965	amod	spiral,prominence
R5531	T8965	T8963	pobj	prominence,of
R5532	T8966	T8965	punct	(,prominence
R5533	T8967	T8965	appos	SP,prominence
R5534	T8968	T8965	punct	),prominence
R5535	T8969	T8962	auxpass	was,measured
R5536	T8970	T8962	advcl	perpendicular,measured
R5537	T8971	T8970	prep	to,perpendicular
R5538	T8972	T8973	det	a,line
R5539	T8973	T8971	pobj	line,to
R554	T1013	T1011	cc	or,fetal
R5540	T8974	T8973	amod	tangential,line
R5541	T8975	T8976	punct	(,dashed
R5542	T8976	T8973	parataxis	dashed,line
R5543	T8977	T8976	punct	),dashed
R5544	T8978	T8979	dep	that,connects
R5545	T8979	T8973	relcl	connects,line
R5546	T8980	T8981	det	the,surface
R5547	T8981	T8979	dobj	surface,connects
R5548	T8982	T8981	prep	of,surface
R5549	T8983	T8984	det	the,sulcus
R555	T1014	T1011	conj	adult,fetal
R5550	T8984	T8982	pobj	sulcus,of
R5551	T8985	T8984	amod	outer,sulcus
R5552	T8986	T8984	punct	(,sulcus
R5553	T8987	T8984	appos	OS,sulcus
R5554	T8988	T8984	punct	),sulcus
R5555	T8989	T8979	prep	with,connects
R5556	T8990	T8991	det	the,layer
R5557	T8991	T8989	pobj	layer,with
R5558	T8992	T8991	amod	basal,layer
R5559	T8993	T8991	prep	of,layer
R556	T1015	T1008	punct	", ",found
R5560	T8994	T8995	compound	stria,vascularis
R5561	T8995	T8993	pobj	vascularis,of
R5562	T8996	T8962	punct	.,measured
R5563	T8998	T8999	nsubjpass	Thickness,measured
R5564	T9000	T8998	prep	of,Thickness
R5565	T9001	T9002	amod	spiral,ligament
R5566	T9002	T9000	pobj	ligament,of
R5567	T9003	T9002	punct	(,ligament
R5568	T9004	T9002	appos	SL,ligament
R5569	T9005	T9002	punct	),ligament
R557	T1016	T1008	advcl	consistent,found
R5570	T9006	T8999	auxpass	was,measured
R5571	T9007	T8999	advcl	perpendicular,measured
R5572	T9008	T9007	prep	to,perpendicular
R5573	T9009	T9010	det	the,line
R5574	T9010	T9008	pobj	line,to
R5575	T9011	T9010	amod	tangential,line
R5576	T9012	T8999	prep	as,measured
R5577	T9013	T9012	pobj	distance,as
R5578	T9014	T9013	prep	between,distance
R5579	T9015	T9016	det	the,surface
R558	T1017	T1016	prep	with,consistent
R5580	T9016	T9014	pobj	surface,between
R5581	T9017	T9016	prep	of,surface
R5582	T9018	T9019	amod	spiral,prominence
R5583	T9019	T9017	pobj	prominence,of
R5584	T9020	T9016	cc	and,surface
R5585	T9021	T9022	det	the,interface
R5586	T9022	T9016	conj	interface,surface
R5587	T9023	T9022	prep	between,interface
R5588	T9024	T9025	amod	spiral,ligament
R5589	T9025	T9023	pobj	ligament,between
R559	T1018	T1019	det	a,cause
R5590	T9026	T9025	cc	and,ligament
R5591	T9027	T9025	conj	bone,ligament
R5592	T9028	T9027	punct	(,bone
R5593	T9029	T9027	appos	B,bone
R5594	T9030	T9027	punct	),bone
R5595	T9031	T8999	punct	.,measured
R5596	T9033	T9034	nsubjpass	Thickness,obtained
R5597	T9035	T9033	prep	of,Thickness
R5598	T9036	T9037	amod	spiral,limbus
R5599	T9037	T9035	pobj	limbus,of
R56	T292	T285	advcl	using,investigated
R560	T1019	T1017	pobj	cause,with
R5600	T9038	T9037	punct	(,limbus
R5601	T9039	T9037	appos	LIM,limbus
R5602	T9040	T9037	punct	),limbus
R5603	T9041	T9034	auxpass	was,obtained
R5604	T9042	T9034	advcl	perpendicular,obtained
R5605	T9043	T9042	prep	to,perpendicular
R5606	T9044	T9045	det	the,surface
R5607	T9045	T9043	pobj	surface,to
R5608	T9046	T9045	prep	of,surface
R5609	T9047	T9048	det	the,bone
R561	T1020	T1019	amod	peripheral,cause
R5610	T9048	T9046	pobj	bone,of
R5611	T9049	T9034	prep	as,obtained
R5612	T9050	T9051	det	a,line
R5613	T9051	T9049	pobj	line,as
R5614	T9052	T9051	amod	tangential,line
R5615	T9053	T9054	dep	that,touches
R5616	T9054	T9051	relcl	touches,line
R5617	T9055	T9056	det	the,sulcus
R5618	T9056	T9054	dobj	sulcus,touches
R5619	T9057	T9056	amod	inner,sulcus
R562	T1021	T1019	prep	of,cause
R5620	T9058	T9056	punct	(,sulcus
R5621	T9059	T9056	appos	IS,sulcus
R5622	T9060	T9056	punct	),sulcus
R5623	T9061	T9054	cc	and,touches
R5624	T9062	T9054	conj	reaches,touches
R5625	T9063	T9062	prep	from,reaches
R5626	T9064	T9065	det	the,surface
R5627	T9065	T9063	pobj	surface,from
R5628	T9066	T9065	prep	of,surface
R5629	T9067	T9068	det	the,limbus
R563	T1022	T1021	pobj	deafness,of
R5630	T9068	T9066	pobj	limbus,of
R5631	T9069	T9068	amod	spiral,limbus
R5632	T9070	T9062	prep	to,reaches
R5633	T9071	T9072	det	the,interface
R5634	T9072	T9070	pobj	interface,to
R5635	T9073	T9072	prep	between,interface
R5636	T9074	T9075	amod	spiral,limbus
R5637	T9075	T9073	pobj	limbus,between
R5638	T9076	T9077	amod	connective,tissue
R5639	T9077	T9075	appos	tissue,limbus
R564	T1023	T1024	punct	[,11
R5640	T9078	T9077	cc	and,tissue
R5641	T9079	T9077	conj	bone,tissue
R5642	T9080	T9034	punct	.,obtained
R5643	T9082	T9083	meta	b,Summary
R5644	T9084	T9083	punct	: ,Summary
R5645	T9085	T9083	punct	.,Summary
R5646	T9087	T9088	nsubj	Data,contributed
R5647	T9089	T9087	prep	from,Data
R5648	T9090	T9091	quantmod	7,8
R5649	T9091	T9093	nummod	8,animals
R565	T1024	T1008	parataxis	11,found
R5650	T9092	T9091	punct	–,8
R5651	T9093	T9089	pobj	animals,from
R5652	T9094	T9088	prep	to,contributed
R5653	T9095	T9096	det	each,column
R5654	T9096	T9094	pobj	column,to
R5655	T9097	T9088	punct	.,contributed
R5658	T9177	T9176	prep	of,Analysis
R5659	T9178	T9179	amod	marginal,cell
R566	T1025	T1024	nummod	2,11
R5660	T9179	T9182	compound	cell,barriers
R5661	T9180	T9178	cc	and,marginal
R5662	T9181	T9178	conj	basal,marginal
R5663	T9182	T9177	pobj	barriers,of
R5664	T9183	T9182	nmod	by,barriers
R5665	T9184	T9182	prep	in,barriers
R5666	T9185	T9186	nmod	Slc26a4,mice
R5667	T9186	T9184	pobj	mice,in
R5668	T9187	T9185	punct	-,Slc26a4
R5669	T9188	T9185	punct	/,Slc26a4
R567	T1026	T1024	punct	",",11
R5670	T9189	T9185	punct	-,Slc26a4
R5671	T9190	T9176	punct	.,Analysis
R5672	T9192	T9193	amod	Tight,junctions
R5673	T9193	T9194	nsubjpass	junctions,visualized
R5674	T9195	T9194	auxpass	were,visualized
R5675	T9196	T9194	prep	by,visualized
R5676	T9197	T9198	compound	F,actin
R5677	T9198	T9196	pobj	actin,by
R5678	T9199	T9194	punct	.,visualized
R5679	T9201	T9202	amod	Whole,mounts
R568	T1027	T1024	punct	],11
R5680	T9202	T9204	nsubjpass	mounts,viewed
R5681	T9203	T9202	punct	-,mounts
R5682	T9205	T9202	prep	of,mounts
R5683	T9206	T9207	compound	stria,vascularis
R5684	T9207	T9205	pobj	vascularis,of
R5685	T9208	T9204	auxpass	were,viewed
R5686	T9209	T9210	preconj	either,from
R5687	T9210	T9204	prep	from,viewed
R5688	T9211	T9212	det	the,side
R5689	T9212	T9210	pobj	side,from
R569	T1028	T1008	punct	.,found
R5690	T9213	T9214	amod	basal,cell
R5691	T9214	T9212	compound	cell,side
R5692	T9215	T9216	punct	(,a
R5693	T9216	T9212	parataxis	a,side
R5694	T9217	T9218	punct	-,f
R5695	T9218	T9216	prep	f,a
R5696	T9219	T9216	punct	),a
R5697	T9220	T9210	cc	or,from
R5698	T9221	T9210	conj	from,from
R5699	T9222	T9223	det	the,side
R57	T293	T292	dobj	mice,using
R570	T1030	T1031	nsubjpass	Expression,found
R5700	T9223	T9221	pobj	side,from
R5701	T9224	T9225	amod	marginal,cell
R5702	T9225	T9223	compound	cell,side
R5703	T9226	T9227	punct	(,g
R5704	T9227	T9223	parataxis	g,side
R5705	T9228	T9229	punct	-,l
R5706	T9229	T9227	prep	l,g
R5707	T9230	T9227	punct	),g
R5708	T9231	T9204	punct	.,viewed
R5709	T9233	T9234	amod	Bright,field
R571	T1032	T1030	prep	of,Expression
R5710	T9234	T9235	compound	field,images
R5711	T9235	T9236	nsubj	images,verify
R5712	T9237	T9238	mark	that,viewed
R5713	T9238	T9236	ccomp	viewed,verify
R5714	T9239	T9240	det	the,area
R5715	T9240	T9238	nsubjpass	area,viewed
R5716	T9241	T9240	amod	same,area
R5717	T9242	T9238	auxpass	was,viewed
R5718	T9243	T9238	prep	from,viewed
R5719	T9244	T9245	preconj	either,side
R572	T1033	T1034	compound	pendrin,mRNA
R5720	T9245	T9243	pobj	side,from
R5721	T9246	T9247	punct	(,b
R5722	T9247	T9236	parataxis	b,verify
R5723	T9248	T9247	cc	and,b
R5724	T9249	T9247	conj	h,b
R5725	T9250	T9247	punct	),b
R5726	T9251	T9236	punct	.,verify
R5727	T9253	T9254	amod	Colored,images
R5728	T9254	T9257	nsubjpass	images,mixed
R5729	T9255	T9256	amod	bright,field
R573	T1034	T1032	pobj	mRNA,of
R5730	T9256	T9254	compound	field,images
R5731	T9258	T9257	auxpass	were,mixed
R5732	T9259	T9257	prep	with,mixed
R5733	T9260	T9259	pobj	images,with
R5734	T9261	T9260	prep	of,images
R5735	T9262	T9263	compound	F,actin
R5736	T9263	T9264	compound	actin,staining
R5737	T9264	T9261	pobj	staining,of
R5738	T9265	T9266	aux	to,indicate
R5739	T9266	T9257	advcl	indicate,mixed
R574	T1035	T1030	prep	in,Expression
R5740	T9267	T9268	det	the,position
R5741	T9268	T9266	dobj	position,indicate
R5742	T9269	T9268	prep	of,position
R5743	T9270	T9271	compound	pigment,granules
R5744	T9271	T9269	pobj	granules,of
R5745	T9272	T9273	punct	(,d
R5746	T9273	T9257	parataxis	d,mixed
R5747	T9274	T9273	punct	", ",d
R5748	T9275	T9273	conj	j,d
R5749	T9276	T9275	punct	", ",j
R575	T1036	T1037	det	the,ear
R5750	T9277	T9275	conj	f,j
R5751	T9278	T9277	cc	and,f
R5752	T9279	T9277	conj	l,f
R5753	T9280	T9273	punct	),d
R5754	T9281	T9257	punct	.,mixed
R5755	T9283	T9284	nsubjpass	Focus,varied
R5756	T9285	T9284	auxpass	was,varied
R5757	T9286	T9287	aux	to,visualize
R5758	T9287	T9284	advcl	visualize,varied
R5759	T9288	T9287	preconj	either,visualize
R576	T1037	T1035	pobj	ear,in
R5760	T9289	T9290	det	the,barrier
R5761	T9290	T9287	dobj	barrier,visualize
R5762	T9291	T9292	amod	marginal,cell
R5763	T9292	T9290	compound	cell,barrier
R5764	T9293	T9294	punct	(,c
R5765	T9294	T9290	parataxis	c,barrier
R5766	T9295	T9294	dep	SMC,c
R5767	T9296	T9294	punct	", ",c
R5768	T9297	T9298	punct	-,d
R5769	T9298	T9294	prep	d,c
R577	T1038	T1037	amod	inner,ear
R5770	T9299	T9294	cc	and,c
R5771	T9300	T9294	conj	i,c
R5772	T9301	T9302	punct	-,j
R5773	T9302	T9300	prep	j,i
R5774	T9303	T9294	punct	),c
R5775	T9304	T9290	cc	or,barrier
R5776	T9305	T9306	det	the,barrier
R5777	T9306	T9290	conj	barrier,barrier
R5778	T9307	T9308	amod	basal,cell
R5779	T9308	T9306	compound	cell,barrier
R578	T1039	T1031	aux	has,found
R5780	T9309	T9310	punct	(,e
R5781	T9310	T9306	parataxis	e,barrier
R5782	T9311	T9310	dep	BC,e
R5783	T9312	T9310	punct	", ",e
R5784	T9313	T9314	punct	-,f
R5785	T9314	T9310	prep	f,e
R5786	T9315	T9310	cc	and,e
R5787	T9316	T9310	conj	k,e
R5788	T9317	T9318	punct	-,l
R5789	T9318	T9316	prep	l,k
R579	T1040	T1031	auxpass	been,found
R5790	T9319	T9310	punct	),e
R5791	T9320	T9284	punct	.,varied
R5792	T9322	T9323	preconj	Both,cell
R5793	T9323	T9326	nsubj	cell,appeared
R5794	T9324	T9323	det	the,cell
R5795	T9325	T9323	amod	marginal,cell
R5796	T9327	T9328	punct	(,e
R5797	T9328	T9323	parataxis	e,cell
R5798	T9329	T9330	punct	-,f
R5799	T9330	T9328	prep	f,e
R58	T294	T295	dep	that,have
R580	T1041	T1031	prep	in,found
R5800	T9331	T9328	punct	),e
R5801	T9332	T9323	cc	and,cell
R5802	T9333	T9334	det	the,cell
R5803	T9334	T9336	compound	cell,barrier
R5804	T9335	T9334	amod	basal,cell
R5805	T9336	T9323	conj	barrier,cell
R5806	T9337	T9338	punct	(,i
R5807	T9338	T9336	parataxis	i,barrier
R5808	T9339	T9340	punct	-,f
R5809	T9340	T9338	prep	f,i
R581	T1042	T1043	amod	several,places
R5810	T9341	T9338	punct	),i
R5811	T9342	T9343	aux	to,be
R5812	T9343	T9326	xcomp	be,appeared
R5813	T9344	T9343	acomp	intact,be
R5814	T9345	T9326	punct	.,appeared
R5815	T9347	T9348	nsubj	It,was
R5816	T9349	T9348	acomp	critical,was
R5817	T9350	T9348	prep	for,was
R5818	T9351	T9352	det	this,finding
R5819	T9352	T9350	pobj	finding,for
R582	T1043	T1041	pobj	places,in
R5820	T9353	T9354	mark	that,block
R5821	T9354	T9348	ccomp	block,was
R5822	T9355	T9354	nsubj	pigmentation,block
R5823	T9356	T9354	aux	did,block
R5824	T9357	T9354	neg	not,block
R5825	T9358	T9359	det	the,path
R5826	T9359	T9354	dobj	path,block
R5827	T9360	T9359	prep	of,path
R5828	T9361	T9362	det	the,laser
R5829	T9362	T9360	pobj	laser,of
R583	T1044	T1043	prep	including,places
R5830	T9363	T9348	punct	.,was
R5831	T9365	T9366	nsubj	Blockage,produces
R5832	T9367	T9365	prep	of,Blockage
R5833	T9368	T9369	det	the,laser
R5834	T9369	T9367	pobj	laser,of
R5835	T9370	T9365	prep	by,Blockage
R5836	T9371	T9370	pobj	pigmentation,by
R5837	T9372	T9373	det	the,impression
R5838	T9373	T9366	dobj	impression,produces
R5839	T9374	T9373	amod	untrue,impression
R584	T1045	T1046	det	the,cochlea
R5840	T9375	T9373	prep	of,impression
R5841	T9376	T9377	det	a,barrier
R5842	T9377	T9375	pobj	barrier,of
R5843	T9378	T9377	amod	discontinuous,barrier
R5844	T9379	T9380	amod	marginal,cell
R5845	T9380	T9377	compound	cell,barrier
R5846	T9381	T9382	punct	(,c
R5847	T9382	T9377	parataxis	c,barrier
R5848	T9383	T9384	punct	-,d
R5849	T9384	T9382	prep	d,c
R585	T1046	T1044	pobj	cochlea,including
R5850	T9385	T9382	punct	),c
R5851	T9386	T9377	cc	or,barrier
R5852	T9387	T9388	amod	basal,cell
R5853	T9388	T9389	compound	cell,barrier
R5854	T9389	T9377	conj	barrier,barrier
R5855	T9390	T9391	punct	(,k
R5856	T9391	T9389	parataxis	k,barrier
R5857	T9392	T9393	punct	-,l
R5858	T9393	T9391	prep	l,k
R5859	T9394	T9391	punct	),k
R586	T1047	T1046	punct	", ",cochlea
R5860	T9395	T9366	punct	.,produces
R5861	T9397	T9398	nsubjpass	Comparison,aided
R5862	T9399	T9397	prep	of,Comparison
R5863	T9400	T9399	pobj	images,of
R5864	T9401	T9398	auxpass	is,aided
R5865	T9402	T9398	prep	by,aided
R5866	T9403	T9402	pcomp	marking,by
R5867	T9404	T9405	det	a,area
R5868	T9405	T9403	dobj	area,marking
R5869	T9406	T9405	amod	significant,area
R587	T1048	T1049	det	the,labyrinth
R5870	T9407	T9403	prep	with,marking
R5871	T9408	T9409	det	a,star
R5872	T9409	T9407	pobj	star,with
R5873	T9410	T9398	punct	.,aided
R5874	T9412	T9413	nsubj	Bars,μm
R5875	T9414	T9413	punct	=,μm
R5876	T9415	T9413	nummod	10,μm
R5877	T9416	T9413	punct	.,μm
R588	T1049	T1046	conj	labyrinth,cochlea
R5880	T9537	T9536	prep	of,Analysis
R5881	T9538	T9539	amod	marginal,barriers
R5882	T9539	T9537	pobj	barriers,of
R5883	T9540	T9538	cc	and,marginal
R5884	T9541	T9538	conj	basal,marginal
R5885	T9542	T9539	compound	cell,barriers
R5886	T9543	T9536	prep	in,Analysis
R5887	T9544	T9545	nmod	Slc26a4,mice
R5888	T9545	T9543	pobj	mice,in
R5889	T9546	T9544	punct	+,Slc26a4
R589	T1050	T1049	amod	vestibular,labyrinth
R5890	T9547	T9544	punct	/,Slc26a4
R5891	T9548	T9544	punct	+,Slc26a4
R5892	T9549	T9544	cc	and,Slc26a4
R5893	T9550	T9544	conj	Slc26a4,Slc26a4
R5894	T9551	T9550	punct	-,Slc26a4
R5895	T9552	T9550	punct	/,Slc26a4
R5896	T9553	T9550	punct	-,Slc26a4
R5897	T9554	T9536	punct	.,Analysis
R5898	T9556	T9557	amod	Tight,junctions
R5899	T9557	T9558	nsubjpass	junctions,visualized
R59	T295	T293	relcl	have,mice
R590	T1051	T1049	cc	and,labyrinth
R5900	T9559	T9558	auxpass	were,visualized
R5901	T9560	T9558	prep	by,visualized
R5902	T9561	T9562	compound	F,actin
R5903	T9562	T9560	pobj	actin,by
R5904	T9563	T9558	prep	in,visualized
R5905	T9564	T9565	amod	whole,mounts
R5906	T9565	T9563	pobj	mounts,in
R5907	T9566	T9565	punct	-,mounts
R5908	T9567	T9565	prep	of,mounts
R5909	T9568	T9569	compound	stria,vascularis
R591	T1052	T1053	det	the,sac
R5910	T9569	T9567	pobj	vascularis,of
R5911	T9570	T9558	punct	.,visualized
R5912	T9572	T9573	amod	Bright,field
R5913	T9573	T9574	compound	field,images
R5914	T9574	T9575	nsubjpass	images,taken
R5915	T9576	T9575	auxpass	were,taken
R5916	T9577	T9578	aux	to,evaluate
R5917	T9578	T9575	advcl	evaluate,taken
R5918	T9579	T9578	dobj	pigmentation,evaluate
R5919	T9580	T9581	punct	(,a
R592	T1053	T1049	conj	sac,labyrinth
R5920	T9581	T9575	parataxis	a,taken
R5921	T9582	T9581	punct	", ",a
R5922	T9583	T9581	conj	d,a
R5923	T9584	T9583	cc	and,d
R5924	T9585	T9583	conj	g,d
R5925	T9586	T9581	punct	),a
R5926	T9587	T9575	punct	.,taken
R5927	T9590	T9591	det	the,barriers
R5928	T9591	T9589	dobj	barriers,Note
R5929	T9592	T9591	amod	intact,barriers
R593	T1054	T1053	amod	endolymphatic,sac
R5930	T9593	T9594	amod	marginal,cell
R5931	T9594	T9591	nmod	cell,barriers
R5932	T9595	T9596	punct	(,b
R5933	T9596	T9594	parataxis	b,cell
R5934	T9597	T9596	punct	),b
R5935	T9598	T9594	cc	and,cell
R5936	T9599	T9600	amod	basal,cell
R5937	T9600	T9594	conj	cell,cell
R5938	T9601	T9602	punct	(,c
R5939	T9602	T9600	parataxis	c,cell
R594	T1055	T1056	punct	[,8
R5940	T9603	T9602	punct	),c
R5941	T9604	T9591	prep	in,barriers
R5942	T9605	T9606	nmod	Slc26a4,mice
R5943	T9606	T9604	pobj	mice,in
R5944	T9607	T9605	punct	+,Slc26a4
R5945	T9608	T9605	punct	/,Slc26a4
R5946	T9609	T9605	punct	+,Slc26a4
R5947	T9610	T9589	punct	.,Note
R5948	T9612	T9613	amod	Minimal,pigmentation
R5949	T9613	T9614	nsubj	pigmentation,compromise
R595	T1056	T1031	parataxis	8,found
R5950	T9615	T9613	prep	of,pigmentation
R5951	T9616	T9617	nmod	Slc26a4,mice
R5952	T9617	T9615	pobj	mice,of
R5953	T9618	T9616	punct	+,Slc26a4
R5954	T9619	T9616	punct	/,Slc26a4
R5955	T9620	T9616	punct	+,Slc26a4
R5956	T9621	T9614	aux	did,compromise
R5957	T9622	T9614	neg	not,compromise
R5958	T9623	T9624	compound	F,actin
R5959	T9624	T9625	compound	actin,localization
R596	T1057	T1056	punct	],8
R5960	T9625	T9614	dobj	localization,compromise
R5961	T9626	T9614	punct	.,compromise
R5962	T9628	T9629	amod	Whole,mounts
R5963	T9629	T9631	nsubjpass	mounts,viewed
R5964	T9630	T9629	punct	-,mounts
R5965	T9632	T9629	prep	of,mounts
R5966	T9633	T9634	compound	stria,vascularis
R5967	T9634	T9632	pobj	vascularis,of
R5968	T9635	T9629	prep	from,mounts
R5969	T9636	T9637	nmod	Slc26a4,mice
R597	T1058	T1031	punct	.,found
R5970	T9637	T9635	pobj	mice,from
R5971	T9638	T9636	punct	-,Slc26a4
R5972	T9639	T9636	punct	/,Slc26a4
R5973	T9640	T9636	punct	-,Slc26a4
R5974	T9641	T9631	auxpass	were,viewed
R5975	T9642	T9643	preconj	either,from
R5976	T9643	T9631	prep	from,viewed
R5977	T9644	T9645	det	the,side
R5978	T9645	T9643	pobj	side,from
R5979	T9646	T9647	amod	basal,cell
R598	T1060	T1061	det	The,location
R5980	T9647	T9645	compound	cell,side
R5981	T9648	T9649	punct	(,e
R5982	T9649	T9645	parataxis	e,side
R5983	T9650	T9651	punct	-,f
R5984	T9651	T9649	prep	f,e
R5985	T9652	T9649	punct	),e
R5986	T9653	T9643	cc	or,from
R5987	T9654	T9643	conj	from,from
R5988	T9655	T9656	det	the,side
R5989	T9656	T9654	pobj	side,from
R599	T1061	T1063	nsubjpass	location,determined
R5990	T9657	T9658	amod	marginal,cell
R5991	T9658	T9656	compound	cell,side
R5992	T9659	T9660	punct	(,h
R5993	T9660	T9656	parataxis	h,side
R5994	T9661	T9662	punct	-,i
R5995	T9662	T9660	prep	i,h
R5996	T9663	T9660	punct	),h
R5997	T9664	T9631	punct	.,viewed
R5998	T9666	T9667	amod	Bright,field
R5999	T9667	T9668	compound	field,images
R60	T296	T297	punct	(,Slc26a4
R600	T1062	T1061	amod	precise,location
R6000	T9668	T9669	nsubj	images,verify
R6001	T9670	T9671	mark	that,viewed
R6002	T9671	T9669	ccomp	viewed,verify
R6003	T9672	T9673	det	the,area
R6004	T9673	T9671	nsubjpass	area,viewed
R6005	T9674	T9673	amod	same,area
R6006	T9675	T9671	auxpass	was,viewed
R6007	T9676	T9671	prep	from,viewed
R6008	T9677	T9678	preconj	either,side
R6009	T9678	T9676	pobj	side,from
R601	T1064	T1061	prep	of,location
R6010	T9679	T9680	punct	(,d
R6011	T9680	T9669	parataxis	d,verify
R6012	T9681	T9680	cc	and,d
R6013	T9682	T9680	conj	g,d
R6014	T9683	T9680	punct	),d
R6015	T9684	T9669	punct	.,verify
R6016	T9686	T9687	nsubjpass	Focus,varied
R6017	T9688	T9687	auxpass	was,varied
R6018	T9689	T9690	aux	to,visualize
R6019	T9690	T9687	advcl	visualize,varied
R602	T1065	T1066	compound	pendrin,expression
R6020	T9691	T9690	preconj	either,visualize
R6021	T9692	T9693	det	the,barrier
R6022	T9693	T9690	dobj	barrier,visualize
R6023	T9694	T9695	amod	marginal,cell
R6024	T9695	T9693	compound	cell,barrier
R6025	T9696	T9697	punct	(,b
R6026	T9697	T9693	parataxis	b,barrier
R6027	T9698	T9697	dep	SMC,b
R6028	T9699	T9697	punct	", ",b
R6029	T9700	T9697	punct	",",b
R603	T1066	T1064	pobj	expression,of
R6030	T9701	T9697	conj	e,b
R6031	T9702	T9701	cc	and,e
R6032	T9703	T9701	conj	h,e
R6033	T9704	T9697	punct	),b
R6034	T9705	T9693	cc	or,barrier
R6035	T9706	T9707	det	the,barrier
R6036	T9707	T9693	conj	barrier,barrier
R6037	T9708	T9709	amod	basal,cell
R6038	T9709	T9707	compound	cell,barrier
R6039	T9710	T9711	punct	(,c
R604	T1067	T1066	compound	protein,expression
R6040	T9711	T9707	parataxis	c,barrier
R6041	T9712	T9711	dep	BC,c
R6042	T9713	T9711	punct	", ",c
R6043	T9714	T9711	punct	",",c
R6044	T9715	T9711	conj	f,c
R6045	T9716	T9715	cc	and,f
R6046	T9717	T9715	conj	i,f
R6047	T9718	T9711	punct	),c
R6048	T9719	T9687	punct	.,varied
R6049	T9721	T9722	preconj	Both,cell
R605	T1068	T1063	punct	", ",determined
R6050	T9722	T9725	nsubj	cell,appeared
R6051	T9723	T9722	det	the,cell
R6052	T9724	T9722	amod	basal,cell
R6053	T9726	T9727	punct	(,f
R6054	T9727	T9722	parataxis	f,cell
R6055	T9728	T9727	punct	),f
R6056	T9729	T9722	cc	and,cell
R6057	T9730	T9731	det	the,cell
R6058	T9731	T9733	nmod	cell,barriers
R6059	T9732	T9731	amod	marginal,cell
R606	T1069	T1063	advmod	however,determined
R6060	T9733	T9722	conj	barriers,cell
R6061	T9734	T9735	punct	(,h
R6062	T9735	T9731	parataxis	h,cell
R6063	T9736	T9735	punct	),h
R6064	T9737	T9738	aux	to,be
R6065	T9738	T9725	xcomp	be,appeared
R6066	T9739	T9738	acomp	intact,be
R6067	T9740	T9725	punct	.,appeared
R6068	T9742	T9743	nsubj	Blockage,produces
R6069	T9744	T9742	prep	of,Blockage
R607	T1070	T1063	punct	", ",determined
R6070	T9745	T9746	det	the,laser
R6071	T9746	T9744	pobj	laser,of
R6072	T9747	T9742	prep	by,Blockage
R6073	T9748	T9747	pobj	pigmentation,by
R6074	T9749	T9750	det	the,impression
R6075	T9750	T9743	dobj	impression,produces
R6076	T9751	T9750	amod	untrue,impression
R6077	T9752	T9750	prep	of,impression
R6078	T9753	T9752	punct	',of
R6079	T9754	T9752	pobj	holes,of
R608	T1071	T1063	aux	has,determined
R6080	T9755	T9754	punct	',holes
R6081	T9756	T9754	prep	in,holes
R6082	T9757	T9758	det	the,barrier
R6083	T9758	T9756	pobj	barrier,in
R6084	T9759	T9760	amod	marginal,cell
R6085	T9760	T9758	compound	cell,barrier
R6086	T9761	T9762	punct	(,e
R6087	T9762	T9758	parataxis	e,barrier
R6088	T9763	T9762	punct	),e
R6089	T9764	T9758	cc	or,barrier
R609	T1072	T1063	neg	not,determined
R6090	T9765	T9766	amod	basal,cell
R6091	T9766	T9767	compound	cell,barrier
R6092	T9767	T9758	conj	barrier,barrier
R6093	T9768	T9769	punct	(,i
R6094	T9769	T9767	parataxis	i,barrier
R6095	T9770	T9769	punct	),i
R6096	T9771	T9743	punct	.,produces
R6097	T9773	T9774	nsubjpass	Comparison,aided
R6098	T9775	T9773	prep	of,Comparison
R6099	T9776	T9775	pobj	images,of
R61	T297	T295	parataxis	Slc26a4,have
R610	T1073	T1063	advmod	yet,determined
R6100	T9777	T9774	auxpass	is,aided
R6101	T9778	T9774	agent	by,aided
R6102	T9779	T9778	pcomp	marking,by
R6103	T9780	T9781	nummod	three,areas
R6104	T9781	T9779	dobj	areas,marking
R6105	T9782	T9781	amod	significant,areas
R6106	T9783	T9779	prep	with,marking
R6107	T9784	T9785	amod	colored,stars
R6108	T9785	T9783	pobj	stars,with
R6109	T9786	T9774	punct	.,aided
R611	T1074	T1063	auxpass	been,determined
R6110	T9788	T9789	nsubj	Bars,μm
R6111	T9790	T9789	punct	=,μm
R6112	T9791	T9789	nummod	10,μm
R6113	T9792	T9789	punct	.,μm
R6116	T9850	T9851	compound	Protein,localization
R6117	T9852	T9851	prep	of,localization
R6118	T9853	T9852	pobj	KCNQ1,of
R6119	T9854	T9853	punct	", ",KCNQ1
R612	T1075	T1063	punct	.,determined
R6120	T9855	T9853	conj	KCNE1,KCNQ1
R6121	T9856	T9855	punct	", ",KCNE1
R6122	T9857	T9855	conj	SLC12A2,KCNE1
R6123	T9858	T9857	cc	and,SLC12A2
R6124	T9859	T9857	conj	GJB2,SLC12A2
R6125	T9860	T9851	prep	in,localization
R6126	T9861	T9862	det	the,wall
R6127	T9862	T9860	pobj	wall,in
R6128	T9863	T9862	amod	cochlear,wall
R6129	T9864	T9862	amod	lateral,wall
R613	T1077	T1078	det	The,variability
R6130	T9865	T9862	prep	of,wall
R6131	T9866	T9867	nmod	Slc26a4,mice
R6132	T9867	T9865	pobj	mice,of
R6133	T9868	T9866	punct	+,Slc26a4
R6134	T9869	T9866	punct	/,Slc26a4
R6135	T9870	T9866	punct	+,Slc26a4
R6136	T9871	T9866	cc	and,Slc26a4
R6137	T9872	T9866	conj	Slc26a4,Slc26a4
R6138	T9873	T9872	punct	-,Slc26a4
R6139	T9874	T9872	punct	/,Slc26a4
R614	T1078	T1079	nsubj	variability,suggest
R6140	T9875	T9872	punct	-,Slc26a4
R6141	T9876	T9851	punct	.,localization
R6142	T9878	T9879	meta	a,μm
R6143	T9880	T9878	punct	-,a
R6144	T9881	T9878	dep	f,a
R6145	T9882	T9879	punct	: ,μm
R6146	T9883	T9879	dep	bars,μm
R6147	T9884	T9879	punct	: ,μm
R6148	T9885	T9879	nummod	10,μm
R6149	T9886	T9879	punct	.,μm
R615	T1080	T1078	prep	of,variability
R6150	T9889	T9888	punct	", ",SMC
R6151	T9890	T9891	amod	strial,cells
R6152	T9891	T9888	appos	cells,SMC
R6153	T9892	T9891	amod	marginal,cells
R6154	T9893	T9888	punct	;,SMC
R6155	T9894	T9888	appos	SV,SMC
R6156	T9895	T9894	punct	", ",SV
R6157	T9896	T9897	compound	stria,vascularis
R6158	T9897	T9894	appos	vascularis,SV
R6159	T9898	T9888	punct	;,SMC
R616	T1081	T1080	pobj	deafness,of
R6160	T9899	T9888	appos	BC,SMC
R6161	T9900	T9899	punct	", ",BC
R6162	T9901	T9902	amod	basal,cells
R6163	T9902	T9899	appos	cells,BC
R6164	T9903	T9888	punct	;,SMC
R6165	T9904	T9888	appos	SL,SMC
R6166	T9905	T9904	punct	", ",SL
R6167	T9906	T9907	amod	spiral,ligament
R6168	T9907	T9904	appos	ligament,SL
R6169	T9908	T9888	punct	.,SMC
R617	T1082	T1078	prep	in,variability
R6170	T9959	T9958	prep	for,Model
R6171	T9960	T9961	det	the,loss
R6172	T9961	T9959	pobj	loss,for
R6173	T9962	T9961	prep	of,loss
R6174	T9963	T9962	pobj	KCNJ10,of
R6175	T9964	T9961	prep	in,loss
R6176	T9965	T9966	det	the,absence
R6177	T9966	T9964	pobj	absence,in
R6178	T9967	T9966	prep	of,absence
R6179	T9968	T9969	compound	pendrin,expression
R618	T1083	T1084	compound	Pendred,syndrome
R6180	T9969	T9967	pobj	expression,of
R6181	T9970	T9961	prep	in,loss
R6182	T9971	T9972	compound	stria,vascularis
R6183	T9972	T9970	pobj	vascularis,in
R6184	T9973	T9958	punct	.,Model
R6185	T9976	T9975	punct	", ",Cys
R6186	T9977	T9975	appos	cysteine,Cys
R6187	T9978	T9975	punct	", ",Cys
R6188	T9979	T9975	appos	Glu,Cys
R6189	T9980	T9979	punct	", ",Glu
R619	T1084	T1082	pobj	syndrome,in
R6190	T9981	T9979	appos	glutamate,Glu
R6191	T9982	T9975	punct	", ",Cys
R6192	T9983	T9975	appos	Gly,Cys
R6193	T9984	T9983	punct	", ",Gly
R6194	T9985	T9983	appos	glycine,Gly
R6195	T9986	T9975	punct	", ",Cys
R6196	T9987	T9975	appos	CA,Cys
R6197	T9988	T9987	punct	", ",CA
R6198	T9989	T9990	amod	carbonic,anhydrase
R6199	T9990	T9987	appos	anhydrase,CA
R62	T298	T297	punct	+,Slc26a4
R620	T1085	T1078	cc	and,variability
R6200	T9991	T9975	punct	", ",Cys
R6201	T9992	T9975	appos	GST,Cys
R6202	T9993	T9992	punct	", ",GST
R6203	T9994	T9995	compound	glutathione,transferase
R6204	T9995	T9992	appos	transferase,GST
R6205	T9996	T9995	punct	-,transferase
R6206	T9997	T9995	compound	S,transferase
R6207	T9998	T9995	punct	-,transferase
R6208	T9999	T9975	punct	", ",Cys
R6209	T10000	T9975	appos	GSH,Cys
R621	T1086	T1087	det	the,observation
R6210	T10001	T10000	punct	", ",GSH
R6211	T10002	T10000	appos	glutathione,GSH
R6212	T10003	T9975	punct	.,Cys
R622	T1087	T1078	conj	observation,variability
R623	T1088	T1089	mark	that,is
R624	T1089	T1087	acl	is,observation
R625	T1090	T1089	nsubj	deafness,is
R626	T1091	T1089	advmod	sometimes,is
R627	T1092	T1089	acomp	late,is
R628	T1093	T1089	prep	in,is
R629	T1094	T1093	pobj	onset,in
R63	T299	T297	punct	/,Slc26a4
R630	T1095	T1096	mark	that,be
R631	T1096	T1079	ccomp	be,suggest
R632	T1097	T1098	compound	pendrin,dysfunction
R633	T1098	T1096	nsubj	dysfunction,be
R634	T1099	T1096	aux	may,be
R635	T1100	T1096	neg	not,be
R636	T1101	T1102	det	the,cause
R637	T1102	T1096	attr	cause,be
R638	T1103	T1102	amod	direct,cause
R639	T1104	T1102	prep	of,cause
R64	T300	T297	punct	+,Slc26a4
R640	T1105	T1104	pobj	deafness,of
R641	T1106	T1079	punct	.,suggest
R642	T1108	T1109	nsubj	It,is
R643	T1110	T1109	acomp	conceivable,is
R644	T1111	T1112	mark	that,favors
R645	T1112	T1109	ccomp	favors,is
R646	T1113	T1114	compound	pendrin,dysfunction
R647	T1114	T1112	nsubj	dysfunction,favors
R648	T1115	T1112	dobj	changes,favors
R649	T1116	T1115	prep	in,changes
R65	T301	T297	punct	),Slc26a4
R650	T1117	T1118	det	the,levels
R651	T1118	T1116	pobj	levels,in
R652	T1119	T1118	compound	expression,levels
R653	T1120	T1118	prep	of,levels
R654	T1121	T1120	pobj	proteins,of
R655	T1122	T1123	dep	that,are
R656	T1123	T1121	relcl	are,proteins
R657	T1124	T1123	acomp	critical,are
R658	T1125	T1124	prep	for,critical
R659	T1126	T1127	det	the,maintenance
R66	T302	T295	cc	or,have
R660	T1127	T1125	pobj	maintenance,for
R661	T1128	T1127	prep	of,maintenance
R662	T1129	T1130	det	the,function
R663	T1130	T1128	pobj	function,of
R664	T1131	T1130	compound	hearing,function
R665	T1132	T1109	punct	.,is
R666	T1134	T1135	amod	Detailed,studies
R667	T1135	T1136	nsubj	studies,become
R668	T1137	T1135	acl	aimed,studies
R669	T1138	T1137	prep	at,aimed
R67	T303	T295	conj	lack,have
R670	T1139	T1138	pcomp	identifying,at
R671	T1140	T1141	det	the,cause
R672	T1141	T1139	dobj	cause,identifying
R673	T1142	T1141	amod	direct,cause
R674	T1143	T1141	prep	of,cause
R675	T1144	T1143	pobj	deafness,of
R676	T1145	T1141	prep	in,cause
R677	T1146	T1147	compound	Pendred,syndrome
R678	T1147	T1145	pobj	syndrome,in
R679	T1148	T1136	aux	have,become
R68	T304	T305	det	a,gene
R680	T1149	T1136	advmod	recently,become
R681	T1150	T1136	acomp	possible,become
R682	T1151	T1136	prep	due,become
R683	T1152	T1151	pcomp	to,due
R684	T1153	T1154	det	the,generation
R685	T1154	T1151	pobj	generation,due
R686	T1155	T1154	prep	of,generation
R687	T1156	T1157	det	a,antibody
R688	T1157	T1155	pobj	antibody,of
R689	T1158	T1159	npadvmod	pendrin,specific
R69	T305	T303	dobj	gene,lack
R690	T1159	T1157	amod	specific,antibody
R691	T1160	T1159	punct	-,specific
R692	T1161	T1157	amod	polyclonal,antibody
R693	T1162	T1163	punct	[,9
R694	T1163	T1154	parataxis	9,generation
R695	T1164	T1163	punct	],9
R696	T1165	T1154	cc	and,generation
R697	T1166	T1167	det	the,development
R698	T1167	T1154	conj	development,generation
R699	T1168	T1167	prep	of,development
R7	T234	T235	nsubj	Loss,abolishes
R70	T306	T305	amod	complete,gene
R700	T1169	T1170	nmod	Slc26a4,mice
R701	T1170	T1168	pobj	mice,of
R702	T1171	T1169	punct	-,Slc26a4
R703	T1172	T1169	punct	/,Slc26a4
R704	T1173	T1169	punct	-,Slc26a4
R705	T1174	T1170	punct	", ",mice
R706	T1175	T1176	dep	which,bear
R707	T1176	T1170	relcl	bear,mice
R708	T1177	T1178	det	a,disruption
R709	T1178	T1176	dobj	disruption,bear
R71	T307	T305	compound	Slc26a4,gene
R710	T1179	T1178	amod	targeted,disruption
R711	T1180	T1178	prep	of,disruption
R712	T1181	T1182	det	the,gene
R713	T1182	T1180	pobj	gene,of
R714	T1183	T1182	compound	mouse,gene
R715	T1184	T1182	compound	Slc26a4,gene
R716	T1185	T1186	punct	[,14
R717	T1186	T1136	parataxis	14,become
R718	T1187	T1186	punct	],14
R719	T1188	T1136	punct	.,become
R72	T308	T309	punct	(,-
R720	T1190	T1191	det	The,aim
R721	T1191	T1192	nsubj	aim,was
R722	T1193	T1191	prep	in,aim
R723	T1194	T1195	det	the,study
R724	T1195	T1193	pobj	study,in
R725	T1196	T1195	amod	present,study
R726	T1197	T1198	aux	to,determine
R727	T1198	T1192	xcomp	determine,was
R728	T1199	T1200	det	the,location
R729	T1200	T1198	dobj	location,determine
R73	T309	T303	punct	-,lack
R730	T1201	T1200	prep	of,location
R731	T1202	T1201	pobj	pendrin,of
R732	T1203	T1200	cc	and,location
R733	T1204	T1205	det	the,cause
R734	T1205	T1200	conj	cause,location
R735	T1206	T1205	prep	of,cause
R736	T1207	T1206	pobj	deafness,of
R737	T1208	T1198	prep	in,determine
R738	T1209	T1210	nmod	Slc26a4,mice
R739	T1210	T1208	pobj	mice,in
R74	T310	T309	nmod	Slc26a4,-
R740	T1211	T1209	punct	-,Slc26a4
R741	T1212	T1209	punct	/,Slc26a4
R742	T1213	T1209	punct	-,Slc26a4
R743	T1214	T1192	punct	.,was
R744	T1314	T1315	det	The,potential
R745	T1315	T1317	nsubjpass	potential,measured
R746	T1316	T1315	amod	endocochlear,potential
R747	T1318	T1315	cc	and,potential
R748	T1319	T1320	det	the,concentrations
R749	T1320	T1315	conj	concentrations,potential
R75	T311	T309	punct	-,-
R750	T1321	T1320	amod	endolymphatic,concentrations
R751	T1322	T1321	cc	and,endolymphatic
R752	T1323	T1321	conj	perilymphatic,endolymphatic
R753	T1324	T1320	nmod	K,concentrations
R754	T1325	T1324	punct	+,K
R755	T1326	T1317	auxpass	were,measured
R756	T1327	T1317	prep	in,measured
R757	T1328	T1329	amod	young,mice
R758	T1329	T1327	pobj	mice,in
R759	T1330	T1329	amod	adult,mice
R76	T312	T309	punct	/,-
R760	T1331	T1332	punct	(,month
R761	T1332	T1329	parataxis	month,mice
R762	T1333	T1334	quantmod	1,4
R763	T1334	T1332	nummod	4,month
R764	T1335	T1334	punct	–,4
R765	T1336	T1332	prep	of,month
R766	T1337	T1336	pobj	age,of
R767	T1338	T1332	punct	),month
R768	T1339	T1340	dep	that,have
R769	T1340	T1329	relcl	have,mice
R77	T313	T309	punct	),-
R770	T1341	T1340	preconj	either,have
R771	T1342	T1343	punct	(,Slc26a4
R772	T1343	T1340	parataxis	Slc26a4,have
R773	T1344	T1343	punct	+,Slc26a4
R774	T1345	T1343	punct	/,Slc26a4
R775	T1346	T1343	punct	+,Slc26a4
R776	T1347	T1343	punct	),Slc26a4
R777	T1348	T1340	cc	or,have
R778	T1349	T1340	conj	lack,have
R779	T1350	T1351	punct	(,Slc26a4
R78	T314	T285	punct	.,investigated
R780	T1351	T1349	parataxis	Slc26a4,lack
R781	T1352	T1351	punct	-,Slc26a4
R782	T1353	T1351	punct	/,Slc26a4
R783	T1354	T1351	punct	-,Slc26a4
R784	T1355	T1351	punct	),Slc26a4
R785	T1356	T1357	det	a,gene
R786	T1357	T1349	dobj	gene,lack
R787	T1358	T1357	amod	functional,gene
R788	T1359	T1357	prep	for,gene
R789	T1360	T1359	pobj	pendrin,for
R79	T318	T319	nsubjpass	Expression,determined
R790	T1361	T1362	punct	[,14
R791	T1362	T1349	parataxis	14,lack
R792	T1363	T1362	punct	],14
R793	T1364	T1317	punct	.,measured
R794	T1366	T1367	det	The,strain
R795	T1367	T1369	nsubjpass	strain,chosen
R796	T1368	T1367	compound	mouse,strain
R797	T1370	T1371	compound	129Sv,Ev
R798	T1371	T1367	appos	Ev,strain
R799	T1372	T1371	punct	/,Ev
R8	T237	T234	prep	of,Loss
R80	T320	T318	prep	of,Expression
R800	T1373	T1374	punct	(,Taconic
R801	T1374	T1367	parataxis	Taconic,strain
R802	T1375	T1374	punct	", ",Taconic
R803	T1376	T1374	npadvmod	Germantown,Taconic
R804	T1377	T1374	punct	", ",Taconic
R805	T1378	T1374	npadvmod	NY,Taconic
R806	T1379	T1374	punct	),Taconic
R807	T1380	T1369	auxpass	was,chosen
R808	T1381	T1369	prep	as,chosen
R809	T1382	T1383	det	the,source
R81	T321	T320	pobj	pendrin,of
R810	T1383	T1381	pobj	source,as
R811	T1384	T1383	prep	of,source
R812	T1385	T1386	nmod	Slc26a4,controls
R813	T1386	T1384	pobj	controls,of
R814	T1387	T1385	punct	+,Slc26a4
R815	T1388	T1385	punct	/,Slc26a4
R816	T1389	T1385	punct	+,Slc26a4
R817	T1390	T1369	punct	", ",chosen
R818	T1391	T1392	mark	since,propagated
R819	T1392	T1369	advcl	propagated,chosen
R82	T322	T321	cc	and,pendrin
R820	T1393	T1394	nmod	Slc26a4,mice
R821	T1394	T1392	nsubjpass	mice,propagated
R822	T1395	T1393	punct	-,Slc26a4
R823	T1396	T1393	punct	/,Slc26a4
R824	T1397	T1393	punct	-,Slc26a4
R825	T1398	T1392	auxpass	were,propagated
R826	T1399	T1392	prep	in,propagated
R827	T1400	T1401	nmod	the,strain
R828	T1401	T1399	pobj	strain,in
R829	T1402	T1401	det	this,strain
R83	T323	T324	amod	other,proteins
R830	T1403	T1392	cc	and,propagated
R831	T1404	T1392	conj	generated,propagated
R832	T1405	T1404	advcl	using,generated
R833	T1406	T1407	det	a,line
R834	T1407	T1405	dobj	line,using
R835	T1408	T1409	compound	stem,cell
R836	T1409	T1407	compound	cell,line
R837	T1410	T1407	acl	derived,line
R838	T1411	T1410	prep	from,derived
R839	T1412	T1413	compound	129Sv,Ev
R84	T324	T321	conj	proteins,pendrin
R840	T1413	T1411	pobj	Ev,from
R841	T1414	T1413	punct	/,Ev
R842	T1415	T1369	punct	.,chosen
R843	T1417	T1418	nsubj	Slc26a4,were
R844	T1419	T1417	punct	+,Slc26a4
R845	T1420	T1417	punct	/,Slc26a4
R846	T1421	T1417	punct	+,Slc26a4
R847	T1422	T1417	cc	and,Slc26a4
R848	T1423	T1417	conj	Slc26a4,Slc26a4
R849	T1424	T1423	punct	-,Slc26a4
R85	T325	T319	auxpass	was,determined
R850	T1425	T1423	punct	/,Slc26a4
R851	T1426	T1423	punct	-,Slc26a4
R852	T1427	T1418	attr	agouti,were
R853	T1428	T1418	punct	.,were
R854	T1430	T1431	nsubj	They,differ
R855	T1432	T1431	aux	did,differ
R856	T1433	T1431	neg	not,differ
R857	T1434	T1431	prep	in,differ
R858	T1435	T1436	compound	coat,color
R859	T1436	T1434	pobj	color,in
R86	T326	T319	prep	by,determined
R860	T1437	T1431	punct	.,differ
R861	T1439	T1440	nsubjpass	Differences,verified
R862	T1441	T1439	prep	in,Differences
R863	T1442	T1441	pobj	pigmentation,in
R864	T1443	T1440	auxpass	were,verified
R865	T1444	T1440	advcl	using,verified
R866	T1445	T1446	amod	coisogenic,Slc26a4
R867	T1446	T1444	dobj	Slc26a4,using
R868	T1447	T1448	npadvmod	age,matched
R869	T1448	T1446	amod	matched,Slc26a4
R87	T327	T328	amod	confocal,immunocytochemistry
R870	T1449	T1448	punct	-,matched
R871	T1450	T1446	punct	+,Slc26a4
R872	T1451	T1446	punct	/,Slc26a4
R873	T1452	T1446	punct	+,Slc26a4
R874	T1453	T1446	cc	and,Slc26a4
R875	T1454	T1446	conj	Slc26a4,Slc26a4
R876	T1455	T1454	punct	-,Slc26a4
R877	T1456	T1454	punct	/,Slc26a4
R878	T1457	T1454	punct	-,Slc26a4
R879	T1458	T1459	dep	that,bred
R88	T328	T326	pobj	immunocytochemistry,by
R880	T1459	T1446	relcl	bred,Slc26a4
R881	T1460	T1459	auxpass	were,bred
R882	T1461	T1459	prep	in,bred
R883	T1462	T1461	pobj	parallel,in
R884	T1463	T1440	punct	.,verified
R885	T1465	T1466	nsubjpass	Expression,studied
R886	T1467	T1465	prep	of,Expression
R887	T1468	T1469	amod	key,proteins
R888	T1469	T1467	pobj	proteins,of
R889	T1470	T1469	acl	involved,proteins
R89	T329	T319	punct	.,determined
R890	T1471	T1470	prep	in,involved
R891	T1472	T1473	det	the,generation
R892	T1473	T1471	pobj	generation,in
R893	T1474	T1473	prep	of,generation
R894	T1475	T1476	det	the,potential
R895	T1476	T1474	pobj	potential,of
R896	T1477	T1476	amod	endocochlear,potential
R897	T1478	T1473	cc	and,generation
R898	T1479	T1480	det	the,transport
R899	T1480	T1473	conj	transport,generation
R9	T238	T239	compound	KCNJ10,expression
R90	T331	T332	nsubjpass	Expression,determined
R900	T1481	T1480	prep	of,transport
R901	T1482	T1481	pobj	K,of
R902	T1483	T1482	punct	+,K
R903	T1484	T1466	auxpass	was,studied
R904	T1485	T1466	advcl	using,studied
R905	T1486	T1487	amod	confocal,immunocytochemistry
R906	T1487	T1485	dobj	immunocytochemistry,using
R907	T1488	T1487	cc	and,immunocytochemistry
R908	T1489	T1490	amod	quantitative,PCR
R909	T1490	T1487	conj	PCR,immunocytochemistry
R91	T333	T331	prep	of,Expression
R910	T1491	T1490	compound	RT,PCR
R911	T1492	T1490	punct	-,PCR
R912	T1493	T1466	punct	.,studied
R913	T1495	T1496	nmod	K,secretion
R914	T1496	T1498	nsubjpass	secretion,measured
R915	T1497	T1495	punct	+,K
R916	T1499	T1496	cc	and,secretion
R917	T1500	T1501	det	the,generation
R918	T1501	T1496	conj	generation,secretion
R919	T1502	T1501	prep	of,generation
R92	T334	T333	pobj	mRNA,of
R920	T1503	T1504	det	the,potential
R921	T1504	T1502	pobj	potential,of
R922	T1505	T1504	amod	endocochlear,potential
R923	T1506	T1498	auxpass	were,measured
R924	T1507	T1498	advcl	using,measured
R925	T1508	T1509	amod	electrophysiological,techniques
R926	T1509	T1507	dobj	techniques,using
R927	T1510	T1498	punct	.,measured
R928	T1512	T1513	det	All,experiments
R929	T1513	T1514	nsubjpass	experiments,approved
R93	T335	T332	auxpass	was,determined
R930	T1515	T1514	auxpass	were,approved
R931	T1516	T1514	agent	by,approved
R932	T1517	T1518	det	the,Committee
R933	T1518	T1516	pobj	Committee,by
R934	T1519	T1520	nmod	Institutional,Animal
R935	T1520	T1518	nmod	Animal,Committee
R936	T1521	T1520	appos	Care,Animal
R937	T1522	T1521	cc	and,Care
R938	T1523	T1521	conj	Use,Care
R939	T1524	T1518	prep	of,Committee
R94	T336	T332	prep	by,determined
R940	T1525	T1526	compound	Kansas,University
R941	T1526	T1524	pobj	University,of
R942	T1527	T1526	compound	State,University
R943	T1528	T1514	punct	.,approved
R944	T1678	T1679	amod	Confocal,immunocytochemistry
R945	T1681	T1682	nsubjpass	Animals,anesthetized
R946	T1683	T1682	auxpass	were,anesthetized
R947	T1684	T1682	advmod	deeply,anesthetized
R948	T1685	T1682	prep	with,anesthetized
R949	T1686	T1687	compound	sodium,pentobarbital
R95	T337	T338	amod	quantitative,PCR
R950	T1687	T1685	pobj	pentobarbital,with
R951	T1688	T1689	punct	(,mg
R952	T1689	T1687	parataxis	mg,pentobarbital
R953	T1690	T1689	nummod	100,mg
R954	T1691	T1692	punct	/,kg
R955	T1692	T1689	prep	kg,mg
R956	T1693	T1689	advmod	i.p.,mg
R957	T1694	T1689	punct	),mg
R958	T1695	T1682	cc	and,anesthetized
R959	T1696	T1697	advmod	transcardially,perfused
R96	T338	T336	pobj	PCR,by
R960	T1697	T1682	conj	perfused,anesthetized
R961	T1698	T1697	prep	with,perfused
R962	T1699	T1700	det	a,solution
R963	T1700	T1698	pobj	solution,with
R964	T1701	T1702	npadvmod	Cl,free
R965	T1702	T1700	amod	free,solution
R966	T1703	T1701	punct	-,Cl
R967	T1704	T1702	punct	-,free
R968	T1705	T1706	npadvmod	phosphate,buffered
R969	T1706	T1700	amod	buffered,solution
R97	T339	T338	compound	RT,PCR
R970	T1707	T1706	punct	-,buffered
R971	T1708	T1709	nmod	Na,gluconate
R972	T1709	T1700	compound	gluconate,solution
R973	T1710	T1708	punct	-,Na
R974	T1711	T1700	acl	containing,solution
R975	T1712	T1713	nummod	4,%
R976	T1713	T1714	compound	%,paraformaldehyde
R977	T1714	T1711	dobj	paraformaldehyde,containing
R978	T1715	T1682	punct	.,anesthetized
R979	T1717	T1718	amod	Temporal,bones
R98	T340	T338	punct	-,PCR
R980	T1718	T1719	nsubjpass	bones,removed
R981	T1720	T1719	auxpass	were,removed
R982	T1721	T1719	cc	and,removed
R983	T1722	T1723	nsubj	cochleae,fixed
R984	T1723	T1719	conj	fixed,removed
R985	T1724	T1723	prep	by,fixed
R986	T1725	T1726	amod	perilymphatic,perfusion
R987	T1726	T1724	pobj	perfusion,by
R988	T1727	T1723	punct	", ",fixed
R989	T1728	T1723	conj	decalcified,fixed
R99	T341	T332	punct	.,determined
R990	T1729	T1728	prep	in,decalcified
R991	T1730	T1729	pobj	EDTA,in
R992	T1731	T1728	punct	", ",decalcified
R993	T1732	T1728	conj	processed,decalcified
R994	T1733	T1732	prep	through,processed
R995	T1734	T1735	det	a,gradient
R996	T1735	T1733	pobj	gradient,through
R997	T1736	T1735	compound	sucrose,gradient
R998	T1737	T1732	cc	and,processed
R999	T1738	T1732	conj	infiltrated,processed

PMC:516044 JSONTXT 8 Projects

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PMC:516044 JSON TXT 8 Projects