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PMC:441373 / 41676-49764 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T10098 9-20 NN denotes Compilation
T10099 21-24 CC denotes and
T10100 25-33 NN denotes assembly
T10101 34-36 IN denotes of
T10102 37-47 RB denotes previously
T10103 48-63 JJ denotes uncharacterized
T10105 64-68 NN denotes TACC
T10104 69-74 NNS denotes cDNAs
T10106 75-78 CC denotes and
T10107 79-84 NNS denotes genes
T10108 84-282 sentence denotes Corresponding orthologous sequences for TACC, RHAMM, KLP, KIF, TPM and keratins families were identified initially using the TBLASTN program [36] to search the published genomic and cDNA databases.
T10109 85-98 VBG denotes Corresponding
T10111 99-110 JJ denotes orthologous
T10110 111-120 NNS denotes sequences
T10113 121-124 IN denotes for
T10114 125-129 NN denotes TACC
T10116 129-131 , denotes ,
T10117 131-136 NN denotes RHAMM
T10118 136-138 , denotes ,
T10119 138-141 NN denotes KLP
T10120 141-143 , denotes ,
T10121 143-146 NN denotes KIF
T10122 146-148 , denotes ,
T10123 148-151 NN denotes TPM
T10124 152-155 CC denotes and
T10125 156-164 NNS denotes keratins
T10115 165-173 NNS denotes families
T10126 174-178 VBD denotes were
T10112 179-189 VBN denotes identified
T10127 190-199 RB denotes initially
T10128 200-205 VBG denotes using
T10129 206-209 DT denotes the
T10131 210-217 NNP denotes TBLASTN
T10130 218-225 NN denotes program
T10132 226-227 -LRB- denotes [
T10133 227-229 CD denotes 36
T10134 229-230 -RRB- denotes ]
T10135 231-233 TO denotes to
T10136 234-240 VB denotes search
T10137 241-244 DT denotes the
T10139 245-254 VBN denotes published
T10140 255-262 JJ denotes genomic
T10141 263-266 CC denotes and
T10142 267-271 NN denotes cDNA
T10138 272-281 NNS denotes databases
T10143 281-282 . denotes .
T10144 282-402 sentence denotes For Takifugu rubripes, gene predictions were produced by the Ensembl automated pipeline [37] and the JGI blast server .
T10145 283-286 IN denotes For
T10147 287-295 NNP denotes Takifugu
T10148 296-304 NNP denotes rubripes
T10149 304-306 , denotes ,
T10150 306-310 NN denotes gene
T10151 311-322 NNS denotes predictions
T10152 323-327 VBD denotes were
T10146 328-336 VBN denotes produced
T10153 337-339 IN denotes by
T10154 340-343 DT denotes the
T10156 344-351 NNP denotes Ensembl
T10157 352-361 VBN denotes automated
T10155 362-370 NN denotes pipeline
T10158 371-372 -LRB- denotes [
T10159 372-374 CD denotes 37
T10160 374-375 -RRB- denotes ]
T10161 376-379 CC denotes and
T10162 380-383 DT denotes the
T10164 384-387 NN denotes JGI
T10165 388-393 NN denotes blast
T10163 394-400 NN denotes server
T10166 401-402 . denotes .
T10167 402-512 sentence denotes DNA sequences covering the homology regions were extracted and analyzed by Genscan to obtain potential exons.
T10168 403-406 NN denotes DNA
T10169 407-416 NNS denotes sequences
T10171 417-425 VBG denotes covering
T10172 426-429 DT denotes the
T10174 430-438 NN denotes homology
T10173 439-446 NNS denotes regions
T10175 447-451 VBD denotes were
T10170 452-461 VBN denotes extracted
T10176 462-465 CC denotes and
T10177 466-474 VBN denotes analyzed
T10178 475-477 IN denotes by
T10179 478-485 NNP denotes Genscan
T10180 486-488 TO denotes to
T10181 489-495 VB denotes obtain
T10182 496-505 JJ denotes potential
T10183 506-511 NNS denotes exons
T10184 511-512 . denotes .
T10185 512-655 sentence denotes In some cases, exons were added or modified based on the best similarity of translated peptides to the corresponding mouse and human proteins.
T10186 513-515 IN denotes In
T10188 516-520 DT denotes some
T10189 521-526 NNS denotes cases
T10190 526-528 , denotes ,
T10191 528-533 NNS denotes exons
T10192 534-538 VBD denotes were
T10187 539-544 VBN denotes added
T10193 545-547 CC denotes or
T10194 548-556 VBN denotes modified
T10195 557-562 VBN denotes based
T10196 563-565 IN denotes on
T10197 566-569 DT denotes the
T10199 570-574 JJS denotes best
T10198 575-585 NN denotes similarity
T10200 586-588 IN denotes of
T10201 589-599 VBN denotes translated
T10202 600-608 NNS denotes peptides
T10203 609-611 IN denotes to
T10204 612-615 DT denotes the
T10206 616-629 VBG denotes corresponding
T10207 630-635 NN denotes mouse
T10208 636-639 CC denotes and
T10209 640-645 JJ denotes human
T10205 646-654 NN denotes proteins
T10210 654-655 . denotes .
T10211 655-833 sentence denotes For regions with low sequence similarity, genomic sequences from the fresh water pufferfish, Tetraodon nigroviridis were used as additional means to verify the predicted exons.
T10212 656-659 IN denotes For
T10214 660-667 NNS denotes regions
T10215 668-672 IN denotes with
T10216 673-676 JJ denotes low
T10218 677-685 NN denotes sequence
T10217 686-696 NN denotes similarity
T10219 696-698 , denotes ,
T10220 698-705 JJ denotes genomic
T10221 706-715 NNS denotes sequences
T10222 717-721 IN denotes from
T10223 722-725 DT denotes the
T10225 726-731 JJ denotes fresh
T10226 732-737 NN denotes water
T10224 738-748 NN denotes pufferfish
T10227 748-750 , denotes ,
T10228 750-759 NNP denotes Tetraodon
T10229 760-772 NNP denotes nigroviridis
T10230 773-777 VBD denotes were
T10213 778-782 VBN denotes used
T10231 783-785 IN denotes as
T10232 786-796 JJ denotes additional
T10233 797-802 NNS denotes means
T10234 803-805 TO denotes to
T10235 806-812 VB denotes verify
T10236 813-816 DT denotes the
T10238 817-826 VBN denotes predicted
T10237 827-832 NNS denotes exons
T10239 832-833 . denotes .
T10240 833-1133 sentence denotes Due to the variability of the central region of vertebrate TACC3 cDNAs (see text), to further confirm prediction of the Takifugu rubripes TACC3, full length cDNAs corresponding to the Danio rerio TACC3 (IMAGE clones 2639991, 2640369 and 3724452) were also obtained from A.T.C.C. and fully sequenced.
T10241 834-837 IN denotes Due
T10243 838-840 IN denotes to
T10244 841-844 DT denotes the
T10245 845-856 NN denotes variability
T10246 857-859 IN denotes of
T10247 860-863 DT denotes the
T10249 864-871 JJ denotes central
T10248 872-878 NN denotes region
T10250 879-881 IN denotes of
T10251 882-892 NN denotes vertebrate
T10253 893-898 NN denotes TACC3
T10252 899-904 NNS denotes cDNAs
T10254 905-906 -LRB- denotes (
T10255 906-909 VB denotes see
T10256 910-914 NN denotes text
T10257 914-915 -RRB- denotes )
T10258 915-917 , denotes ,
T10259 917-919 TO denotes to
T10261 920-927 RB denotes further
T10260 928-935 VB denotes confirm
T10262 936-946 NN denotes prediction
T10263 947-949 IN denotes of
T10264 950-953 DT denotes the
T10266 954-962 NNP denotes Takifugu
T10265 963-971 NNP denotes rubripes
T10267 972-977 NN denotes TACC3
T10268 977-979 , denotes ,
T10269 979-983 JJ denotes full
T10270 984-990 NN denotes length
T10271 991-996 NNS denotes cDNAs
T10272 997-1010 VBG denotes corresponding
T10273 1011-1013 IN denotes to
T10274 1014-1017 DT denotes the
T10276 1018-1023 NNP denotes Danio
T10277 1024-1029 NNP denotes rerio
T10275 1030-1035 NN denotes TACC3
T10278 1036-1037 -LRB- denotes (
T10280 1037-1042 NN denotes IMAGE
T10281 1043-1049 NNS denotes clones
T10279 1050-1057 CD denotes 2639991
T10282 1057-1059 , denotes ,
T10283 1059-1066 CD denotes 2640369
T10284 1067-1070 CC denotes and
T10285 1071-1078 CD denotes 3724452
T10286 1078-1079 -RRB- denotes )
T10287 1080-1084 VBD denotes were
T10288 1085-1089 RB denotes also
T10242 1090-1098 VBN denotes obtained
T10289 1099-1103 IN denotes from
T10290 1104-1112 NN denotes A.T.C.C.
T10291 1113-1116 CC denotes and
T10292 1117-1122 RB denotes fully
T10293 1123-1132 VBN denotes sequenced
T10294 1132-1133 . denotes .
T10295 1133-1311 sentence denotes Potential paralogous chromosomal segments and scaffold were identified by searching the public databases deposited at NCBI and at the Human Genome Mapping Project, Cambridge UK.
T10296 1134-1143 JJ denotes Potential
T10298 1144-1154 JJ denotes paralogous
T10299 1155-1166 JJ denotes chromosomal
T10297 1167-1175 NNS denotes segments
T10301 1176-1179 CC denotes and
T10302 1180-1188 NN denotes scaffold
T10303 1189-1193 VBD denotes were
T10300 1194-1204 VBN denotes identified
T10304 1205-1207 IN denotes by
T10305 1208-1217 VBG denotes searching
T10306 1218-1221 DT denotes the
T10308 1222-1228 JJ denotes public
T10307 1229-1238 NNS denotes databases
T10309 1239-1248 VBN denotes deposited
T10310 1249-1251 IN denotes at
T10311 1252-1256 NNP denotes NCBI
T10312 1257-1260 CC denotes and
T10313 1261-1263 IN denotes at
T10314 1264-1267 DT denotes the
T10316 1268-1273 NNP denotes Human
T10317 1274-1280 NNP denotes Genome
T10318 1281-1288 NNP denotes Mapping
T10315 1289-1296 NNP denotes Project
T10319 1296-1298 , denotes ,
T10320 1298-1307 NNP denotes Cambridge
T10321 1308-1310 NNP denotes UK
T10322 1310-1311 . denotes .
T10439 1313-1320 NN denotes Cloning
T10440 1321-1323 IN denotes of
T10441 1324-1334 NN denotes vertebrate
T10442 1335-1339 NN denotes TACC
T10443 1340-1345 NNS denotes cDNAs
T10444 1345-1610 sentence denotes The rabbit TACC3 was cloned by rt-PCR using the "TACC4" specific primer T4RACE2 [9] (5'-cccgaactgctccaggtaatcgatctc-3') and a consensus primer, T3con2, designed to the region encompassing the vertebrate TACC3 initiator methionine (5'-tatgagtctgcaggtcttaaacgac-3').
T10445 1346-1349 DT denotes The
T10447 1350-1356 NN denotes rabbit
T10446 1357-1362 NN denotes TACC3
T10449 1363-1366 VBD denotes was
T10448 1367-1373 VBN denotes cloned
T10450 1374-1376 IN denotes by
T10451 1377-1379 NN denotes rt
T10453 1379-1380 HYPH denotes -
T10452 1380-1383 NN denotes PCR
T10454 1384-1389 VBG denotes using
T10455 1390-1393 DT denotes the
T10457 1394-1395 `` denotes "
T10459 1395-1400 NN denotes TACC4
T10460 1400-1401 '' denotes "
T10458 1402-1410 JJ denotes specific
T10456 1411-1417 NN denotes primer
T10461 1418-1425 NN denotes T4RACE2
T10462 1426-1427 -LRB- denotes [
T10463 1427-1428 CD denotes 9
T10464 1428-1429 -RRB- denotes ]
T10465 1430-1431 -LRB- denotes (
T10467 1431-1432 CD denotes 5
T10468 1432-1433 SYM denotes '
T10469 1433-1434 HYPH denotes -
T10466 1434-1461 NN denotes cccgaactgctccaggtaatcgatctc
T10470 1461-1462 HYPH denotes -
T10471 1462-1463 CD denotes 3
T10472 1463-1464 SYM denotes '
T10473 1464-1465 -RRB- denotes )
T10474 1466-1469 CC denotes and
T10475 1470-1471 DT denotes a
T10477 1472-1481 NN denotes consensus
T10476 1482-1488 NN denotes primer
T10478 1488-1490 , denotes ,
T10479 1490-1496 NN denotes T3con2
T10480 1496-1498 , denotes ,
T10481 1498-1506 VBN denotes designed
T10482 1507-1509 IN denotes to
T10483 1510-1513 DT denotes the
T10484 1514-1520 NN denotes region
T10485 1521-1533 VBG denotes encompassing
T10486 1534-1537 DT denotes the
T10488 1538-1548 NN denotes vertebrate
T10489 1549-1554 NN denotes TACC3
T10487 1555-1564 NN denotes initiator
T10490 1565-1575 NN denotes methionine
T10491 1576-1577 -LRB- denotes (
T10493 1577-1578 CD denotes 5
T10494 1578-1579 SYM denotes '
T10495 1579-1580 HYPH denotes -
T10492 1580-1605 NN denotes tatgagtctgcaggtcttaaacgac
T10496 1605-1606 HYPH denotes -
T10497 1606-1607 CD denotes 3
T10498 1607-1608 SYM denotes '
T10499 1608-1609 -RRB- denotes )
T10500 1609-1610 . denotes .
T10501 1610-1996 sentence denotes For cloning the mouse Tacc1X cDNA, the primers used were based upon the genomic sequence reported, and the sequence of the IMAGE cDNA clone 4933429K08: T1XF (5'-ccatgttcagtcattggcaggtc-3'), T1XF2 (5'-ctgcagaaccaacagttcaag-3'), T1XR1 (5'-agatctgtgacatcacagctc-3'), T1XR2 (5'-ctcgagtcagttagtcttatccagctt-3'), BB617F (5'-accaccaacttgagtacctg-3') and BB617R (5'-gtatcttgaactgttggttctg-3').
T10502 1611-1614 IN denotes For
T10504 1615-1622 VBG denotes cloning
T10505 1623-1626 DT denotes the
T10507 1627-1632 NN denotes mouse
T10508 1633-1639 NN denotes Tacc1X
T10506 1640-1644 NN denotes cDNA
T10509 1644-1646 , denotes ,
T10510 1646-1649 DT denotes the
T10511 1650-1657 NNS denotes primers
T10512 1658-1662 VBN denotes used
T10513 1663-1667 VBD denotes were
T10503 1668-1673 VBN denotes based
T10514 1674-1678 IN denotes upon
T10515 1679-1682 DT denotes the
T10517 1683-1690 JJ denotes genomic
T10516 1691-1699 NN denotes sequence
T10518 1700-1708 VBN denotes reported
T10519 1708-1710 , denotes ,
T10520 1710-1713 CC denotes and
T10521 1714-1717 DT denotes the
T10522 1718-1726 NN denotes sequence
T10523 1727-1729 IN denotes of
T10524 1730-1733 DT denotes the
T10526 1734-1739 NN denotes IMAGE
T10527 1740-1744 NN denotes cDNA
T10525 1745-1750 NN denotes clone
T10528 1751-1761 NN denotes 4933429K08
T10529 1761-1763 : denotes :
T10530 1763-1767 NN denotes T1XF
T10531 1768-1769 -LRB- denotes (
T10533 1769-1770 CD denotes 5
T10534 1770-1771 SYM denotes '
T10535 1771-1772 HYPH denotes -
T10532 1772-1795 NN denotes ccatgttcagtcattggcaggtc
T10536 1795-1796 HYPH denotes -
T10537 1796-1797 CD denotes 3
T10538 1797-1798 SYM denotes '
T10539 1798-1799 -RRB- denotes )
T10540 1799-1801 , denotes ,
T10541 1801-1806 NN denotes T1XF2
T10542 1807-1808 -LRB- denotes (
T10544 1808-1809 CD denotes 5
T10545 1809-1810 SYM denotes '
T10546 1810-1811 HYPH denotes -
T10543 1811-1832 NN denotes ctgcagaaccaacagttcaag
T10547 1832-1833 HYPH denotes -
T10548 1833-1834 CD denotes 3
T10549 1834-1835 SYM denotes '
T10550 1835-1836 -RRB- denotes )
T10551 1836-1838 , denotes ,
T10552 1838-1843 NN denotes T1XR1
T10553 1844-1845 -LRB- denotes (
T10555 1845-1846 CD denotes 5
T10556 1846-1847 SYM denotes '
T10557 1847-1848 HYPH denotes -
T10554 1848-1869 NN denotes agatctgtgacatcacagctc
T10558 1869-1870 HYPH denotes -
T10559 1870-1871 CD denotes 3
T10560 1871-1872 SYM denotes '
T10561 1872-1873 -RRB- denotes )
T10562 1873-1875 , denotes ,
T10563 1875-1880 NN denotes T1XR2
T10564 1881-1882 -LRB- denotes (
T10566 1882-1883 CD denotes 5
T10567 1883-1884 SYM denotes '
T10568 1884-1885 HYPH denotes -
T10565 1885-1912 NN denotes ctcgagtcagttagtcttatccagctt
T10569 1912-1913 HYPH denotes -
T10570 1913-1914 CD denotes 3
T10571 1914-1915 SYM denotes '
T10572 1915-1916 -RRB- denotes )
T10573 1916-1918 , denotes ,
T10574 1918-1924 NN denotes BB617F
T10575 1925-1926 -LRB- denotes (
T10577 1926-1927 CD denotes 5
T10578 1927-1928 SYM denotes '
T10579 1928-1929 HYPH denotes -
T10576 1929-1949 NN denotes accaccaacttgagtacctg
T10580 1949-1950 HYPH denotes -
T10581 1950-1951 CD denotes 3
T10582 1951-1952 SYM denotes '
T10583 1952-1953 -RRB- denotes )
T10584 1954-1957 CC denotes and
T10585 1958-1964 NN denotes BB617R
T10586 1965-1966 -LRB- denotes (
T10588 1966-1967 CD denotes 5
T10589 1967-1968 SYM denotes '
T10590 1968-1969 HYPH denotes -
T10587 1969-1991 NN denotes gtatcttgaactgttggttctg
T10591 1991-1992 HYPH denotes -
T10592 1992-1993 CD denotes 3
T10593 1993-1994 SYM denotes '
T10594 1994-1995 -RRB- denotes )
T10595 1995-1996 . denotes .
T10596 1996-2203 sentence denotes For analysis of TACC1 splice variants, the forward primers used were located in exon 1b: EF (5'-gagagatgcgaaatcagcg-3'), Exon 1d: X3F (5'-agtcaaagaaggcatctgcag-3'), Exon 1a: 1DF (5'-ccaagttctgcgccatggg-3').
T10597 1997-2000 IN denotes For
T10599 2001-2009 NN denotes analysis
T10600 2010-2012 IN denotes of
T10601 2013-2018 NN denotes TACC1
T10603 2019-2025 NN denotes splice
T10602 2026-2034 NNS denotes variants
T10604 2034-2036 , denotes ,
T10605 2036-2039 DT denotes the
T10607 2040-2047 JJ denotes forward
T10606 2048-2055 NNS denotes primers
T10608 2056-2060 VBN denotes used
T10609 2061-2065 VBD denotes were
T10598 2066-2073 VBN denotes located
T10610 2074-2076 IN denotes in
T10611 2077-2081 NN denotes exon
T10612 2082-2084 CD denotes 1b
T10613 2084-2086 : denotes :
T10614 2086-2088 NN denotes EF
T10616 2089-2090 -LRB- denotes (
T10617 2090-2091 CD denotes 5
T10618 2091-2092 SYM denotes '
T10619 2092-2093 HYPH denotes -
T10615 2093-2112 NN denotes gagagatgcgaaatcagcg
T10620 2112-2113 HYPH denotes -
T10621 2113-2114 CD denotes 3
T10622 2114-2115 SYM denotes '
T10623 2115-2116 -RRB- denotes )
T10624 2116-2118 , denotes ,
T10625 2118-2122 NN denotes Exon
T10626 2123-2125 CD denotes 1d
T10627 2125-2127 : denotes :
T10628 2127-2130 NN denotes X3F
T10630 2131-2132 -LRB- denotes (
T10631 2132-2133 CD denotes 5
T10632 2133-2134 SYM denotes '
T10633 2134-2135 HYPH denotes -
T10629 2135-2156 NN denotes agtcaaagaaggcatctgcag
T10634 2156-2157 HYPH denotes -
T10635 2157-2158 CD denotes 3
T10636 2158-2159 SYM denotes '
T10637 2159-2160 -RRB- denotes )
T10638 2160-2162 , denotes ,
T10639 2162-2166 NN denotes Exon
T10640 2167-2169 CD denotes 1a
T10641 2169-2171 : denotes :
T10642 2171-2174 NN denotes 1DF
T10644 2175-2176 -LRB- denotes (
T10645 2176-2177 CD denotes 5
T10646 2177-2178 SYM denotes '
T10647 2178-2179 HYPH denotes -
T10643 2179-2198 NN denotes ccaagttctgcgccatggg
T10648 2198-2199 HYPH denotes -
T10649 2199-2200 CD denotes 3
T10650 2200-2201 SYM denotes '
T10651 2201-2202 -RRB- denotes )
T10652 2202-2203 . denotes .
T10653 2203-2462 sentence denotes The reverse primers used were: Exon 1: X1R (5'-ggatttggtctcggcttgcgaatc-3'), Exon 2: BX647R (5'-cttgtgattcttggcttttgg-3'), Exon 3: 6SPR (5'-gtcatcgcctcgtcctggagggc-3'), Exon 4a: 1DR (5'-aatttcacttgttcagtagtc-3'), Exon 5: 128R (5'-cctgcttctgaggatgaaaacgc-3').
T10654 2204-2207 DT denotes The
T10656 2208-2215 JJ denotes reverse
T10655 2216-2223 NNS denotes primers
T10658 2224-2228 VBN denotes used
T10657 2229-2233 VBD denotes were
T10659 2233-2235 : denotes :
T10660 2235-2239 NN denotes Exon
T10661 2240-2241 CD denotes 1
T10662 2241-2243 : denotes :
T10663 2243-2246 NN denotes X1R
T10665 2247-2248 -LRB- denotes (
T10666 2248-2249 CD denotes 5
T10667 2249-2250 SYM denotes '
T10668 2250-2251 HYPH denotes -
T10664 2251-2275 NN denotes ggatttggtctcggcttgcgaatc
T10669 2275-2276 HYPH denotes -
T10670 2276-2277 CD denotes 3
T10671 2277-2278 SYM denotes '
T10672 2278-2279 -RRB- denotes )
T10673 2279-2281 , denotes ,
T10674 2281-2285 NN denotes Exon
T10675 2286-2287 CD denotes 2
T10676 2287-2289 : denotes :
T10677 2289-2295 NN denotes BX647R
T10679 2296-2297 -LRB- denotes (
T10680 2297-2298 CD denotes 5
T10681 2298-2299 SYM denotes '
T10682 2299-2300 HYPH denotes -
T10678 2300-2321 NN denotes cttgtgattcttggcttttgg
T10683 2321-2322 HYPH denotes -
T10684 2322-2323 CD denotes 3
T10685 2323-2324 SYM denotes '
T10686 2324-2325 -RRB- denotes )
T10687 2325-2327 , denotes ,
T10688 2327-2331 NN denotes Exon
T10689 2332-2333 CD denotes 3
T10690 2333-2335 : denotes :
T10691 2335-2339 NN denotes 6SPR
T10693 2340-2341 -LRB- denotes (
T10694 2341-2342 CD denotes 5
T10695 2342-2343 SYM denotes '
T10696 2343-2344 HYPH denotes -
T10692 2344-2367 NN denotes gtcatcgcctcgtcctggagggc
T10697 2367-2368 HYPH denotes -
T10698 2368-2369 CD denotes 3
T10699 2369-2370 SYM denotes '
T10700 2370-2371 -RRB- denotes )
T10701 2371-2373 , denotes ,
T10702 2373-2377 NN denotes Exon
T10703 2378-2380 NN denotes 4a
T10704 2380-2382 : denotes :
T10705 2382-2385 NN denotes 1DR
T10707 2386-2387 -LRB- denotes (
T10708 2387-2388 CD denotes 5
T10709 2388-2389 SYM denotes '
T10710 2389-2390 HYPH denotes -
T10706 2390-2411 NN denotes aatttcacttgttcagtagtc
T10711 2411-2412 HYPH denotes -
T10712 2412-2413 CD denotes 3
T10713 2413-2414 SYM denotes '
T10714 2414-2415 -RRB- denotes )
T10715 2415-2417 , denotes ,
T10716 2417-2421 NN denotes Exon
T10717 2422-2423 CD denotes 5
T10718 2423-2425 : denotes :
T10719 2425-2429 NN denotes 128R
T10721 2430-2431 -LRB- denotes (
T10722 2431-2432 CD denotes 5
T10723 2432-2433 SYM denotes '
T10724 2433-2434 HYPH denotes -
T10720 2434-2457 NN denotes cctgcttctgaggatgaaaacgc
T10725 2457-2458 HYPH denotes -
T10726 2458-2459 CD denotes 3
T10727 2459-2460 SYM denotes '
T10728 2460-2461 -RRB- denotes )
T10729 2461-2462 . denotes .
T10730 2462-2595 sentence denotes Rabbit brain poly A+ mRNA, mouse testis and human brain total RNA were obtained from BD Bioscience Clontech (Palo Alto, CA, U.S.A.).
T10731 2463-2469 NN denotes Rabbit
T10732 2470-2475 NN denotes brain
T10734 2476-2480 NN denotes poly
T10735 2481-2483 NN denotes A+
T10733 2484-2488 NN denotes mRNA
T10737 2488-2490 , denotes ,
T10738 2490-2495 NN denotes mouse
T10739 2496-2502 NN denotes testis
T10740 2503-2506 CC denotes and
T10741 2507-2512 JJ denotes human
T10742 2513-2518 NN denotes brain
T10744 2519-2524 JJ denotes total
T10743 2525-2528 NN denotes RNA
T10745 2529-2533 VBD denotes were
T10736 2534-2542 VBN denotes obtained
T10746 2543-2547 IN denotes from
T10747 2548-2550 NNP denotes BD
T10749 2551-2561 NNP denotes Bioscience
T10748 2562-2570 NNP denotes Clontech
T10750 2571-2572 -LRB- denotes (
T10751 2572-2576 NNP denotes Palo
T10752 2577-2581 NNP denotes Alto
T10753 2581-2583 , denotes ,
T10754 2583-2585 NNP denotes CA
T10755 2585-2587 , denotes ,
T10756 2587-2593 NNP denotes U.S.A.
T10757 2593-2594 -RRB- denotes )
T10758 2594-2595 . denotes .
T10759 2595-2765 sentence denotes Reverse transcription and PCR was performed as previously described, using either 1 μg of total RNA or 50 ng of poly A+ mRNA as template for first strand cDNA synthesis.
T10760 2596-2603 JJ denotes Reverse
T10761 2604-2617 NN denotes transcription
T10763 2618-2621 CC denotes and
T10764 2622-2625 NN denotes PCR
T10765 2626-2629 VBD denotes was
T10762 2630-2639 VBN denotes performed
T10766 2640-2642 IN denotes as
T10768 2643-2653 RB denotes previously
T10767 2654-2663 VBN denotes described
T10769 2663-2665 , denotes ,
T10770 2665-2670 VBG denotes using
T10771 2671-2677 CC denotes either
T10773 2678-2679 CD denotes 1
T10772 2680-2682 NN denotes μg
T10774 2683-2685 IN denotes of
T10775 2686-2691 JJ denotes total
T10776 2692-2695 NN denotes RNA
T10777 2696-2698 CC denotes or
T10778 2699-2701 CD denotes 50
T10779 2702-2704 NNS denotes ng
T10780 2705-2707 IN denotes of
T10781 2708-2712 NN denotes poly
T10782 2713-2715 NN denotes A+
T10783 2716-2720 NN denotes mRNA
T10784 2721-2723 IN denotes as
T10785 2724-2732 NN denotes template
T10786 2733-2736 IN denotes for
T10787 2737-2742 JJ denotes first
T10788 2743-2749 NN denotes strand
T10789 2750-2754 NN denotes cDNA
T10790 2755-2764 NN denotes synthesis
T10791 2764-2765 . denotes .
T10792 2765-2881 sentence denotes PCR products were cloned into pCR2.1 (Invitrogen, Carlsbad CA, U.S.A.) and transformed into InvαF' competent cells.
T10793 2766-2769 NN denotes PCR
T10794 2770-2778 NNS denotes products
T10796 2779-2783 VBD denotes were
T10795 2784-2790 VBN denotes cloned
T10797 2791-2795 IN denotes into
T10798 2796-2802 NN denotes pCR2.1
T10799 2803-2804 -LRB- denotes (
T10800 2804-2814 NNP denotes Invitrogen
T10801 2814-2816 , denotes ,
T10802 2816-2824 NNP denotes Carlsbad
T10803 2825-2827 NNP denotes CA
T10804 2827-2829 , denotes ,
T10805 2829-2835 NNP denotes U.S.A.
T10806 2835-2836 -RRB- denotes )
T10807 2837-2840 CC denotes and
T10808 2841-2852 VBN denotes transformed
T10809 2853-2857 IN denotes into
T10810 2858-2863 NN denotes InvαF
T10812 2863-2864 POS denotes '
T10811 2865-2874 JJ denotes competent
T10813 2875-2880 NNS denotes cells
T10814 2880-2881 . denotes .
T10815 2881-2975 sentence denotes Plasmid inserts were sequenced by the Roswell Park Cancer Institute Biopolymer Core Facility.
T10816 2882-2889 NN denotes Plasmid
T10817 2890-2897 NNS denotes inserts
T10819 2898-2902 VBD denotes were
T10818 2903-2912 VBN denotes sequenced
T10820 2913-2915 IN denotes by
T10821 2916-2919 DT denotes the
T10823 2920-2927 NNP denotes Roswell
T10824 2928-2932 NNP denotes Park
T10826 2933-2939 NNP denotes Cancer
T10825 2940-2949 NNP denotes Institute
T10827 2950-2960 NNP denotes Biopolymer
T10828 2961-2965 NNP denotes Core
T10822 2966-2974 NNP denotes Facility
T10829 2974-2975 . denotes .
T10982 2977-2987 NN denotes Deposition
T10983 2988-2990 IN denotes of
T10984 2991-3001 NN denotes nucleotide
T10985 3002-3011 NNS denotes sequences
T10986 3011-3363 sentence denotes Sequences from this article have been deposited in the GenBank database with the following accession numbers: Homo sapiens TACC1 short isoform S (AY177411), Mus musculus TACC1 short isoform S (AY177412), Mus musculus TACC1 long isoform A (AY177413), Mus musculus TACC2s (AY177410), Oryctolagus cuniculus TACC3 (AY161270), Danio rerio TACC3 (AY170618).
T10987 3012-3021 NNS denotes Sequences
T10989 3022-3026 IN denotes from
T10990 3027-3031 DT denotes this
T10991 3032-3039 NN denotes article
T10992 3040-3044 VBP denotes have
T10993 3045-3049 VBN denotes been
T10988 3050-3059 VBN denotes deposited
T10994 3060-3062 IN denotes in
T10995 3063-3066 DT denotes the
T10997 3067-3074 NNP denotes GenBank
T10996 3075-3083 NN denotes database
T10998 3084-3088 IN denotes with
T10999 3089-3092 DT denotes the
T11001 3093-3102 VBG denotes following
T11002 3103-3112 NN denotes accession
T11000 3113-3120 NNS denotes numbers
T11003 3120-3122 : denotes :
T11004 3122-3126 NNP denotes Homo
T11005 3127-3134 NNP denotes sapiens
T11007 3135-3140 NN denotes TACC1
T11008 3141-3146 JJ denotes short
T11009 3147-3154 NN denotes isoform
T11006 3155-3156 NN denotes S
T11010 3157-3158 -LRB- denotes (
T11011 3158-3166 NN denotes AY177411
T11012 3166-3167 -RRB- denotes )
T11013 3167-3169 , denotes ,
T11014 3169-3172 NNP denotes Mus
T11015 3173-3181 NNP denotes musculus
T11017 3182-3187 NN denotes TACC1
T11018 3188-3193 JJ denotes short
T11019 3194-3201 NN denotes isoform
T11016 3202-3203 NN denotes S
T11020 3204-3205 -LRB- denotes (
T11021 3205-3213 NN denotes AY177412
T11022 3213-3214 -RRB- denotes )
T11023 3214-3216 , denotes ,
T11024 3216-3219 NNP denotes Mus
T11025 3220-3228 NNP denotes musculus
T11027 3229-3234 NN denotes TACC1
T11028 3235-3239 JJ denotes long
T11029 3240-3247 NN denotes isoform
T11026 3248-3249 NN denotes A
T11030 3250-3251 -LRB- denotes (
T11031 3251-3259 NN denotes AY177413
T11032 3259-3260 -RRB- denotes )
T11033 3260-3262 , denotes ,
T11034 3262-3265 NNP denotes Mus
T11035 3266-3274 NNP denotes musculus
T11036 3275-3281 NNS denotes TACC2s
T11037 3282-3283 -LRB- denotes (
T11038 3283-3291 NN denotes AY177410
T11039 3291-3292 -RRB- denotes )
T11040 3292-3294 , denotes ,
T11041 3294-3305 NNP denotes Oryctolagus
T11042 3306-3315 NNP denotes cuniculus
T11043 3316-3321 NN denotes TACC3
T11044 3322-3323 -LRB- denotes (
T11045 3323-3331 NN denotes AY161270
T11046 3331-3332 -RRB- denotes )
T11047 3332-3334 , denotes ,
T11048 3334-3339 NNP denotes Danio
T11049 3340-3345 NNP denotes rerio
T11050 3346-3351 NN denotes TACC3
T11051 3352-3353 -LRB- denotes (
T11052 3353-3361 NN denotes AY170618
T11053 3361-3362 -RRB- denotes )
T11054 3362-3363 . denotes .
T11055 3363-4077 sentence denotes Annotations submitted to the Third party annotation database at NCBI are as follows: Rattus norvegus TACC1 long isoform A (BK001653), Takifugu rubripes TACC1A (BK000666/BK000667), Takifugu rubripes TACC1B (BK000664), Mus musculus TACC2l (BK001495), Rattus norvegus TACC2l (BK001658), Rattus norvegus TACC2s (BK001657), Takifugu rubripes TACC2l (BK000690), Rattus norvegus TACC3 (BK001491), Gallus gallus TACC3 (BK001482), Silurana tropicalis TACC3 (BK001481),Takifugu rubripes TACC3 (BK000649), Takifugu rubripes RHAMM (BK000676), Ciona intestinalis RHAMM (BK001479), Takifugu rubripes Keratin (BK000677), Takifugu rubripes TPM1 (BAC10576), Ciona intestinalis Kif3b (BK001492), Ciona intestinalis klp2 (BK001493).
T11056 3364-3375 NNS denotes Annotations
T11058 3376-3385 VBN denotes submitted
T11059 3386-3388 IN denotes to
T11060 3389-3392 DT denotes the
T11062 3393-3398 JJ denotes Third
T11063 3399-3404 NN denotes party
T11064 3405-3415 NN denotes annotation
T11061 3416-3424 NN denotes database
T11065 3425-3427 IN denotes at
T11066 3428-3432 NNP denotes NCBI
T11057 3433-3436 VBP denotes are
T11067 3437-3439 IN denotes as
T11068 3440-3447 VBZ denotes follows
T11069 3447-3449 : denotes :
T11070 3449-3455 NNP denotes Rattus
T11071 3456-3464 NNP denotes norvegus
T11073 3465-3470 NN denotes TACC1
T11074 3471-3475 JJ denotes long
T11075 3476-3483 NN denotes isoform
T11072 3484-3485 NN denotes A
T11076 3486-3487 -LRB- denotes (
T11077 3487-3495 NN denotes BK001653
T11078 3495-3496 -RRB- denotes )
T11079 3496-3498 , denotes ,
T11080 3498-3506 NNP denotes Takifugu
T11081 3507-3515 NNP denotes rubripes
T11082 3516-3522 NN denotes TACC1A
T11083 3523-3524 -LRB- denotes (
T11085 3524-3532 NN denotes BK000666
T11086 3532-3533 HYPH denotes /
T11084 3533-3541 NN denotes BK000667
T11087 3541-3542 -RRB- denotes )
T11088 3542-3544 , denotes ,
T11089 3544-3552 NNP denotes Takifugu
T11090 3553-3561 NNP denotes rubripes
T11091 3562-3568 NN denotes TACC1B
T11092 3569-3570 -LRB- denotes (
T11093 3570-3578 NN denotes BK000664
T11094 3578-3579 -RRB- denotes )
T11095 3579-3581 , denotes ,
T11096 3581-3584 NNP denotes Mus
T11097 3585-3593 NNP denotes musculus
T11098 3594-3600 NN denotes TACC2l
T11099 3601-3602 -LRB- denotes (
T11100 3602-3610 NN denotes BK001495
T11101 3610-3611 -RRB- denotes )
T11102 3611-3613 , denotes ,
T11103 3613-3619 NNP denotes Rattus
T11104 3620-3628 NNP denotes norvegus
T11105 3629-3635 NN denotes TACC2l
T11106 3636-3637 -LRB- denotes (
T11107 3637-3645 NN denotes BK001658
T11108 3645-3646 -RRB- denotes )
T11109 3646-3648 , denotes ,
T11110 3648-3654 NNP denotes Rattus
T11111 3655-3663 NNP denotes norvegus
T11112 3664-3670 NNS denotes TACC2s
T11113 3671-3672 -LRB- denotes (
T11114 3672-3680 NN denotes BK001657
T11115 3680-3681 -RRB- denotes )
T11116 3681-3683 , denotes ,
T11117 3683-3691 NNP denotes Takifugu
T11118 3692-3700 NNP denotes rubripes
T11119 3701-3707 NN denotes TACC2l
T11120 3708-3709 -LRB- denotes (
T11121 3709-3717 NN denotes BK000690
T11122 3717-3718 -RRB- denotes )
T11123 3718-3720 , denotes ,
T11124 3720-3726 NNP denotes Rattus
T11125 3727-3735 NNP denotes norvegus
T11126 3736-3741 NN denotes TACC3
T11127 3742-3743 -LRB- denotes (
T11128 3743-3751 NN denotes BK001491
T11129 3751-3752 -RRB- denotes )
T11130 3752-3754 , denotes ,
T11131 3754-3760 NNP denotes Gallus
T11132 3761-3767 NNP denotes gallus
T11133 3768-3773 NN denotes TACC3
T11134 3774-3775 -LRB- denotes (
T11135 3775-3783 NN denotes BK001482
T11136 3783-3784 -RRB- denotes )
T11137 3784-3786 , denotes ,
T11138 3786-3794 NNP denotes Silurana
T11139 3795-3805 NNP denotes tropicalis
T11140 3806-3811 NN denotes TACC3
T11141 3812-3813 -LRB- denotes (
T11142 3813-3821 NN denotes BK001481
T11143 3821-3822 -RRB- denotes )
T11144 3822-3823 , denotes ,
T11145 3823-3831 NNP denotes Takifugu
T11146 3832-3840 NNP denotes rubripes
T11147 3841-3846 NN denotes TACC3
T11148 3847-3848 -LRB- denotes (
T11149 3848-3856 NN denotes BK000649
T11150 3856-3857 -RRB- denotes )
T11151 3857-3859 , denotes ,
T11152 3859-3867 NNP denotes Takifugu
T11153 3868-3876 NNP denotes rubripes
T11154 3877-3882 NN denotes RHAMM
T11155 3883-3884 -LRB- denotes (
T11156 3884-3892 NN denotes BK000676
T11157 3892-3893 -RRB- denotes )
T11158 3893-3895 , denotes ,
T11159 3895-3900 NNP denotes Ciona
T11160 3901-3913 NNP denotes intestinalis
T11161 3914-3919 NN denotes RHAMM
T11162 3920-3921 -LRB- denotes (
T11163 3921-3929 NN denotes BK001479
T11164 3929-3930 -RRB- denotes )
T11165 3930-3932 , denotes ,
T11166 3932-3940 NNP denotes Takifugu
T11167 3941-3949 NNP denotes rubripes
T11168 3950-3957 NN denotes Keratin
T11169 3958-3959 -LRB- denotes (
T11170 3959-3967 NN denotes BK000677
T11171 3967-3968 -RRB- denotes )
T11172 3968-3970 , denotes ,
T11173 3970-3978 NNP denotes Takifugu
T11174 3979-3987 NNP denotes rubripes
T11175 3988-3992 NN denotes TPM1
T11176 3993-3994 -LRB- denotes (
T11177 3994-4002 NN denotes BAC10576
T11178 4002-4003 -RRB- denotes )
T11179 4003-4005 , denotes ,
T11180 4005-4010 NNP denotes Ciona
T11181 4011-4023 NNP denotes intestinalis
T11182 4024-4029 NN denotes Kif3b
T11183 4030-4031 -LRB- denotes (
T11184 4031-4039 NN denotes BK001492
T11185 4039-4040 -RRB- denotes )
T11186 4040-4042 , denotes ,
T11187 4042-4047 NNP denotes Ciona
T11188 4048-4060 NNP denotes intestinalis
T11189 4061-4065 NN denotes klp2
T11190 4066-4067 -LRB- denotes (
T11191 4067-4075 NN denotes BK001493
T11192 4075-4076 -RRB- denotes )
T11193 4076-4077 . denotes .
T11434 4079-4091 JJ denotes Phylogenetic
T11435 4092-4100 NN denotes analysis
T11436 4100-4584 sentence denotes In order to examine evolutionary relationships of proteins containing coiled coil domains, protein sequences representing the major members of this super family, including TACC, RHAMM, KLP, keratin and tropomyosin from available vertebrates and their recognizable orthologues from the urochordate Ciona intestinalis, Drosophila melanogaster, C. elegans and Saccharomyces cerevisiae were either directly retrieved from NCBI sequence databases, newly predicted or isolated (see above).
T11437 4101-4103 IN denotes In
T11439 4104-4109 NN denotes order
T11440 4110-4112 TO denotes to
T11441 4113-4120 VB denotes examine
T11442 4121-4133 JJ denotes evolutionary
T11443 4134-4147 NNS denotes relationships
T11444 4148-4150 IN denotes of
T11445 4151-4159 NN denotes proteins
T11446 4160-4170 VBG denotes containing
T11447 4171-4177 VBN denotes coiled
T11448 4178-4182 NN denotes coil
T11449 4183-4190 NNS denotes domains
T11450 4190-4192 , denotes ,
T11451 4192-4199 NN denotes protein
T11452 4200-4209 NNS denotes sequences
T11453 4210-4222 VBG denotes representing
T11454 4223-4226 DT denotes the
T11456 4227-4232 JJ denotes major
T11455 4233-4240 NNS denotes members
T11457 4241-4243 IN denotes of
T11458 4244-4248 DT denotes this
T11460 4249-4254 JJ denotes super
T11459 4255-4261 NN denotes family
T11461 4261-4263 , denotes ,
T11462 4263-4272 VBG denotes including
T11463 4273-4277 NN denotes TACC
T11464 4277-4279 , denotes ,
T11465 4279-4284 NN denotes RHAMM
T11466 4284-4286 , denotes ,
T11467 4286-4289 NN denotes KLP
T11468 4289-4291 , denotes ,
T11469 4291-4298 NN denotes keratin
T11470 4299-4302 CC denotes and
T11471 4303-4314 NN denotes tropomyosin
T11472 4315-4319 IN denotes from
T11473 4320-4329 JJ denotes available
T11474 4330-4341 NNS denotes vertebrates
T11475 4342-4345 CC denotes and
T11476 4346-4351 PRP$ denotes their
T11478 4352-4364 JJ denotes recognizable
T11477 4365-4376 NNS denotes orthologues
T11479 4377-4381 IN denotes from
T11480 4382-4385 DT denotes the
T11481 4386-4397 NN denotes urochordate
T11482 4398-4403 NNP denotes Ciona
T11483 4404-4416 NNP denotes intestinalis
T11484 4416-4418 , denotes ,
T11485 4418-4428 NNP denotes Drosophila
T11486 4429-4441 NNP denotes melanogaster
T11487 4441-4443 , denotes ,
T11488 4443-4445 NNP denotes C.
T11489 4446-4453 NNP denotes elegans
T11490 4454-4457 CC denotes and
T11491 4458-4471 NNP denotes Saccharomyces
T11492 4472-4482 NNP denotes cerevisiae
T11493 4483-4487 VBD denotes were
T11494 4488-4494 CC denotes either
T11495 4495-4503 RB denotes directly
T11438 4504-4513 VBN denotes retrieved
T11496 4514-4518 IN denotes from
T11497 4519-4523 NN denotes NCBI
T11499 4524-4532 NN denotes sequence
T11498 4533-4542 NNS denotes databases
T11500 4542-4544 , denotes ,
T11501 4544-4549 RB denotes newly
T11502 4550-4559 VBN denotes predicted
T11503 4560-4562 CC denotes or
T11504 4563-4571 VBN denotes isolated
T11505 4572-4573 -LRB- denotes (
T11506 4573-4576 VB denotes see
T11507 4577-4582 RB denotes above
T11508 4582-4583 -RRB- denotes )
T11509 4583-4584 . denotes .
T11510 4584-4914 sentence denotes Species abbreviations are as follows: hs (Homo sapiens), mm (Mus musculus), rn (Rattus norvegus), oc (Oryctolagus cuniculus), gg (Gallus gallus), xl (Xenopus laevis), st (Silurana tropicalis), tr (Takifugu rubripes), dr (Danio rerio), ci (Ciona intestinalis), dm (D. melanogaster), ce (C. elegans), sc (Saccharomyces cerevisiae).
T11511 4585-4592 NN denotes Species
T11512 4593-4606 NNS denotes abbreviations
T11513 4607-4610 VBP denotes are
T11514 4611-4613 IN denotes as
T11515 4614-4621 VBZ denotes follows
T11516 4621-4623 : denotes :
T11517 4623-4625 NNP denotes hs
T11518 4626-4627 -LRB- denotes (
T11519 4627-4631 NNP denotes Homo
T11520 4632-4639 NNP denotes sapiens
T11521 4639-4640 -RRB- denotes )
T11522 4640-4642 , denotes ,
T11523 4642-4644 NNP denotes mm
T11524 4645-4646 -LRB- denotes (
T11525 4646-4649 NNP denotes Mus
T11526 4650-4658 NNP denotes musculus
T11527 4658-4659 -RRB- denotes )
T11528 4659-4661 , denotes ,
T11529 4661-4663 NNP denotes rn
T11530 4664-4665 -LRB- denotes (
T11531 4665-4671 NNP denotes Rattus
T11532 4672-4680 NNP denotes norvegus
T11533 4680-4681 -RRB- denotes )
T11534 4681-4683 , denotes ,
T11535 4683-4685 NNP denotes oc
T11536 4686-4687 -LRB- denotes (
T11537 4687-4698 NNP denotes Oryctolagus
T11538 4699-4708 NNP denotes cuniculus
T11539 4708-4709 -RRB- denotes )
T11540 4709-4711 , denotes ,
T11541 4711-4713 NNP denotes gg
T11542 4714-4715 -LRB- denotes (
T11543 4715-4721 NNP denotes Gallus
T11544 4722-4728 NNP denotes gallus
T11545 4728-4729 -RRB- denotes )
T11546 4729-4731 , denotes ,
T11547 4731-4733 NNP denotes xl
T11548 4734-4735 -LRB- denotes (
T11549 4735-4742 NNP denotes Xenopus
T11550 4743-4749 NNP denotes laevis
T11551 4749-4750 -RRB- denotes )
T11552 4750-4752 , denotes ,
T11553 4752-4754 NNP denotes st
T11554 4755-4756 -LRB- denotes (
T11555 4756-4764 NNP denotes Silurana
T11556 4765-4775 NNP denotes tropicalis
T11557 4775-4776 -RRB- denotes )
T11558 4776-4778 , denotes ,
T11559 4778-4780 NNP denotes tr
T11560 4781-4782 -LRB- denotes (
T11561 4782-4790 NNP denotes Takifugu
T11562 4791-4799 NNP denotes rubripes
T11563 4799-4800 -RRB- denotes )
T11564 4800-4802 , denotes ,
T11565 4802-4804 NNP denotes dr
T11566 4805-4806 -LRB- denotes (
T11567 4806-4811 NNP denotes Danio
T11568 4812-4817 NNP denotes rerio
T11569 4817-4818 -RRB- denotes )
T11570 4818-4820 , denotes ,
T11571 4820-4822 NNP denotes ci
T11572 4823-4824 -LRB- denotes (
T11573 4824-4829 NNP denotes Ciona
T11574 4830-4842 NNP denotes intestinalis
T11575 4842-4843 -RRB- denotes )
T11576 4843-4845 , denotes ,
T11577 4845-4847 NN denotes dm
T11578 4848-4849 -LRB- denotes (
T11579 4849-4851 NNP denotes D.
T11580 4852-4864 NNP denotes melanogaster
T11581 4864-4865 -RRB- denotes )
T11582 4865-4867 , denotes ,
T11583 4867-4869 NNP denotes ce
T11584 4870-4871 -LRB- denotes (
T11585 4871-4873 NNP denotes C.
T11586 4874-4881 NNP denotes elegans
T11587 4881-4882 -RRB- denotes )
T11588 4882-4884 , denotes ,
T11589 4884-4886 NNP denotes sc
T11590 4887-4888 -LRB- denotes (
T11591 4888-4901 NNP denotes Saccharomyces
T11592 4902-4912 NNP denotes cerevisiae
T11593 4912-4913 -RRB- denotes )
T11594 4913-4914 . denotes .
T11595 4914-5882 sentence denotes The sequences identified above and the following protein or predicted translations were used for phylogenetic analysis: hsTACC1A (NP_006274), hsTACC2l (AAO62630) hsTACC2s (AAO62629), hsTACC3 (NP_006333), mmTACC3 (Q9JJ11), xlMaskin (Q9PTG8), dmTACC (AAF52099), ceTAC1 (NP_497059), scSPC72 (NP_009352), hsRHAMM (NP_036616), mmRHAMM (NP_038580), rnRHAMM (NP_037096), drRHAMM (AAQ97980), hsKeratin (CAB76828), mmKeratin (A61368), rnKeratin (XP_235679), hsTPM1 (NP_000357), mmTPM1 (NP_077745), rnTPM1 (NP_62004, drTPM1 (NP_571180) dmTPM1 (P06754), ceTPM (NP_493540) scTPM1 (P17536), hsKLP2 (BAB03309), rnKIF15 (AAP44513), xlKLP2 (CAA08879), dmKLP2 (NP_476818), ceKLP18 (AA034669), hsKIF3A (Q9Y496), mmKIF3A (NP_032469), rnKIF3A (XP_340797), xlKIF3A (CAA08879), ceKLP11 (NP_741473), ciKIF3 (ci0100148992), hsKIF3B (NP_004789), mmKIF3B (NP_004789), rnKIF3B (XP_215883), dmKIF3B (NP_524029), hsKIF3C (NP_002245), mmKIF3C (NP_032471), rnKIF3C (NP_445938), dmKIF3C (NP_651939).
T11596 4915-4918 DT denotes The
T11597 4919-4928 NNS denotes sequences
T11599 4929-4939 VBN denotes identified
T11600 4940-4945 RB denotes above
T11601 4946-4949 CC denotes and
T11602 4950-4953 DT denotes the
T11604 4954-4963 VBG denotes following
T11605 4964-4971 NN denotes protein
T11606 4972-4974 CC denotes or
T11607 4975-4984 VBN denotes predicted
T11603 4985-4997 NNS denotes translations
T11608 4998-5002 VBD denotes were
T11598 5003-5007 VBN denotes used
T11609 5008-5011 IN denotes for
T11610 5012-5024 JJ denotes phylogenetic
T11611 5025-5033 NN denotes analysis
T11612 5033-5035 : denotes :
T11613 5035-5043 NN denotes hsTACC1A
T11614 5044-5045 -LRB- denotes (
T11615 5045-5054 NN denotes NP_006274
T11616 5054-5055 -RRB- denotes )
T11617 5055-5057 , denotes ,
T11618 5057-5065 NN denotes hsTACC2l
T11619 5066-5067 -LRB- denotes (
T11620 5067-5075 NN denotes AAO62630
T11621 5075-5076 -RRB- denotes )
T11622 5077-5085 NN denotes hsTACC2s
T11623 5086-5087 -LRB- denotes (
T11624 5087-5095 NN denotes AAO62629
T11625 5095-5096 -RRB- denotes )
T11626 5096-5098 , denotes ,
T11627 5098-5105 NN denotes hsTACC3
T11628 5106-5107 -LRB- denotes (
T11629 5107-5116 NN denotes NP_006333
T11630 5116-5117 -RRB- denotes )
T11631 5117-5119 , denotes ,
T11632 5119-5126 NN denotes mmTACC3
T11633 5127-5128 -LRB- denotes (
T11634 5128-5134 NN denotes Q9JJ11
T11635 5134-5135 -RRB- denotes )
T11636 5135-5137 , denotes ,
T11637 5137-5145 NN denotes xlMaskin
T11638 5146-5147 -LRB- denotes (
T11639 5147-5153 NN denotes Q9PTG8
T11640 5153-5154 -RRB- denotes )
T11641 5154-5156 , denotes ,
T11642 5156-5162 NN denotes dmTACC
T11643 5163-5164 -LRB- denotes (
T11644 5164-5172 NN denotes AAF52099
T11645 5172-5173 -RRB- denotes )
T11646 5173-5175 , denotes ,
T11647 5175-5181 NN denotes ceTAC1
T11648 5182-5183 -LRB- denotes (
T11649 5183-5192 NN denotes NP_497059
T11650 5192-5193 -RRB- denotes )
T11651 5193-5195 , denotes ,
T11652 5195-5202 NN denotes scSPC72
T11653 5203-5204 -LRB- denotes (
T11654 5204-5213 NN denotes NP_009352
T11655 5213-5214 -RRB- denotes )
T11656 5214-5216 , denotes ,
T11657 5216-5223 NN denotes hsRHAMM
T11658 5224-5225 -LRB- denotes (
T11659 5225-5234 NN denotes NP_036616
T11660 5234-5235 -RRB- denotes )
T11661 5235-5237 , denotes ,
T11662 5237-5244 NN denotes mmRHAMM
T11663 5245-5246 -LRB- denotes (
T11664 5246-5255 NN denotes NP_038580
T11665 5255-5256 -RRB- denotes )
T11666 5256-5258 , denotes ,
T11667 5258-5265 NN denotes rnRHAMM
T11668 5266-5267 -LRB- denotes (
T11669 5267-5276 NN denotes NP_037096
T11670 5276-5277 -RRB- denotes )
T11671 5277-5279 , denotes ,
T11672 5279-5286 NN denotes drRHAMM
T11673 5287-5288 -LRB- denotes (
T11674 5288-5296 NN denotes AAQ97980
T11675 5296-5297 -RRB- denotes )
T11676 5297-5299 , denotes ,
T11677 5299-5308 NN denotes hsKeratin
T11678 5309-5310 -LRB- denotes (
T11679 5310-5318 NN denotes CAB76828
T11680 5318-5319 -RRB- denotes )
T11681 5319-5321 , denotes ,
T11682 5321-5330 NN denotes mmKeratin
T11683 5331-5332 -LRB- denotes (
T11684 5332-5338 NN denotes A61368
T11685 5338-5339 -RRB- denotes )
T11686 5339-5341 , denotes ,
T11687 5341-5350 NN denotes rnKeratin
T11688 5351-5352 -LRB- denotes (
T11689 5352-5361 NN denotes XP_235679
T11690 5361-5362 -RRB- denotes )
T11691 5362-5364 , denotes ,
T11692 5364-5370 NN denotes hsTPM1
T11693 5371-5372 -LRB- denotes (
T11694 5372-5381 NN denotes NP_000357
T11695 5381-5382 -RRB- denotes )
T11696 5382-5384 , denotes ,
T11697 5384-5390 NN denotes mmTPM1
T11698 5391-5392 -LRB- denotes (
T11699 5392-5401 NN denotes NP_077745
T11700 5401-5402 -RRB- denotes )
T11701 5402-5404 , denotes ,
T11702 5404-5410 NN denotes rnTPM1
T11703 5411-5412 -LRB- denotes (
T11704 5412-5420 NN denotes NP_62004
T11705 5420-5422 , denotes ,
T11706 5422-5428 NN denotes drTPM1
T11707 5429-5430 -LRB- denotes (
T11708 5430-5439 NN denotes NP_571180
T11709 5439-5440 -RRB- denotes )
T11710 5441-5447 NN denotes dmTPM1
T11711 5448-5449 -LRB- denotes (
T11712 5449-5455 NN denotes P06754
T11713 5455-5456 -RRB- denotes )
T11714 5456-5458 , denotes ,
T11715 5458-5463 NN denotes ceTPM
T11716 5464-5465 -LRB- denotes (
T11717 5465-5474 NN denotes NP_493540
T11718 5474-5475 -RRB- denotes )
T11719 5476-5482 NN denotes scTPM1
T11720 5483-5484 -LRB- denotes (
T11721 5484-5490 NN denotes P17536
T11722 5490-5491 -RRB- denotes )
T11723 5491-5493 , denotes ,
T11724 5493-5499 NN denotes hsKLP2
T11725 5500-5501 -LRB- denotes (
T11726 5501-5509 NN denotes BAB03309
T11727 5509-5510 -RRB- denotes )
T11728 5510-5512 , denotes ,
T11729 5512-5519 NN denotes rnKIF15
T11730 5520-5521 -LRB- denotes (
T11731 5521-5529 NN denotes AAP44513
T11732 5529-5530 -RRB- denotes )
T11733 5530-5532 , denotes ,
T11734 5532-5538 NN denotes xlKLP2
T11735 5539-5540 -LRB- denotes (
T11736 5540-5548 NN denotes CAA08879
T11737 5548-5549 -RRB- denotes )
T11738 5549-5551 , denotes ,
T11739 5551-5557 NN denotes dmKLP2
T11740 5558-5559 -LRB- denotes (
T11741 5559-5568 NN denotes NP_476818
T11742 5568-5569 -RRB- denotes )
T11743 5569-5571 , denotes ,
T11744 5571-5578 NN denotes ceKLP18
T11745 5579-5580 -LRB- denotes (
T11746 5580-5588 NN denotes AA034669
T11747 5588-5589 -RRB- denotes )
T11748 5589-5591 , denotes ,
T11749 5591-5598 NN denotes hsKIF3A
T11750 5599-5600 -LRB- denotes (
T11751 5600-5606 NN denotes Q9Y496
T11752 5606-5607 -RRB- denotes )
T11753 5607-5609 , denotes ,
T11754 5609-5616 NN denotes mmKIF3A
T11755 5617-5618 -LRB- denotes (
T11756 5618-5627 NN denotes NP_032469
T11757 5627-5628 -RRB- denotes )
T11758 5628-5630 , denotes ,
T11759 5630-5637 NN denotes rnKIF3A
T11760 5638-5639 -LRB- denotes (
T11761 5639-5648 NN denotes XP_340797
T11762 5648-5649 -RRB- denotes )
T11763 5649-5651 , denotes ,
T11764 5651-5658 NN denotes xlKIF3A
T11765 5659-5660 -LRB- denotes (
T11766 5660-5668 NN denotes CAA08879
T11767 5668-5669 -RRB- denotes )
T11768 5669-5671 , denotes ,
T11769 5671-5678 NN denotes ceKLP11
T11770 5679-5680 -LRB- denotes (
T11771 5680-5689 NN denotes NP_741473
T11772 5689-5690 -RRB- denotes )
T11773 5690-5692 , denotes ,
T11774 5692-5698 NN denotes ciKIF3
T11775 5699-5700 -LRB- denotes (
T11776 5700-5712 NN denotes ci0100148992
T11777 5712-5713 -RRB- denotes )
T11778 5713-5715 , denotes ,
T11779 5715-5722 NN denotes hsKIF3B
T11780 5723-5724 -LRB- denotes (
T11781 5724-5733 NN denotes NP_004789
T11782 5733-5734 -RRB- denotes )
T11783 5734-5736 , denotes ,
T11784 5736-5743 NN denotes mmKIF3B
T11785 5744-5745 -LRB- denotes (
T11786 5745-5754 NN denotes NP_004789
T11787 5754-5755 -RRB- denotes )
T11788 5755-5757 , denotes ,
T11789 5757-5764 NN denotes rnKIF3B
T11790 5765-5766 -LRB- denotes (
T11791 5766-5775 NN denotes XP_215883
T11792 5775-5776 -RRB- denotes )
T11793 5776-5778 , denotes ,
T11794 5778-5785 NN denotes dmKIF3B
T11795 5786-5787 -LRB- denotes (
T11796 5787-5796 NN denotes NP_524029
T11797 5796-5797 -RRB- denotes )
T11798 5797-5799 , denotes ,
T11799 5799-5806 NN denotes hsKIF3C
T11800 5807-5808 -LRB- denotes (
T11801 5808-5817 NN denotes NP_002245
T11802 5817-5818 -RRB- denotes )
T11803 5818-5820 , denotes ,
T11804 5820-5827 NN denotes mmKIF3C
T11805 5828-5829 -LRB- denotes (
T11806 5829-5838 NN denotes NP_032471
T11807 5838-5839 -RRB- denotes )
T11808 5839-5841 , denotes ,
T11809 5841-5848 NN denotes rnKIF3C
T11810 5849-5850 -LRB- denotes (
T11811 5850-5859 NN denotes NP_445938
T11812 5859-5860 -RRB- denotes )
T11813 5860-5862 , denotes ,
T11814 5862-5869 NN denotes dmKIF3C
T11815 5870-5871 -LRB- denotes (
T11816 5871-5880 NN denotes NP_651939
T11817 5880-5881 -RRB- denotes )
T11818 5881-5882 . denotes .
T11819 5882-5950 sentence denotes These protein sequences were initially aligned with CLUSTAL X [38].
T11820 5883-5888 DT denotes These
T11822 5889-5896 NN denotes protein
T11821 5897-5906 NNS denotes sequences
T11824 5907-5911 VBD denotes were
T11825 5912-5921 RB denotes initially
T11823 5922-5929 VBN denotes aligned
T11826 5930-5934 IN denotes with
T11827 5935-5942 NNP denotes CLUSTAL
T11828 5943-5944 NNP denotes X
T11829 5945-5946 -LRB- denotes [
T11830 5946-5948 CD denotes 38
T11831 5948-5949 -RRB- denotes ]
T11832 5949-5950 . denotes .
T11833 5950-6286 sentence denotes Minor adjustments to certain regions of the alignment for optimization purposes were made based on pairwise alignments, the output saved in PHYLIP format, after which, the distances between proteins were calculated using Poisson correction and the Unrooted trees were inferred with the NJ method and then displayed using TreeView [39].
T11834 5951-5956 JJ denotes Minor
T11835 5957-5968 NNS denotes adjustments
T11837 5969-5971 IN denotes to
T11838 5972-5979 JJ denotes certain
T11839 5980-5987 NNS denotes regions
T11840 5988-5990 IN denotes of
T11841 5991-5994 DT denotes the
T11842 5995-6004 NN denotes alignment
T11843 6005-6008 IN denotes for
T11844 6009-6021 NN denotes optimization
T11845 6022-6030 NNS denotes purposes
T11846 6031-6035 VBD denotes were
T11836 6036-6040 VBN denotes made
T11848 6041-6046 VBN denotes based
T11849 6047-6049 IN denotes on
T11850 6050-6058 JJ denotes pairwise
T11851 6059-6069 NNS denotes alignments
T11852 6069-6071 , denotes ,
T11853 6071-6074 DT denotes the
T11854 6075-6081 NN denotes output
T11847 6082-6087 VBD denotes saved
T11855 6088-6090 IN denotes in
T11856 6091-6097 NN denotes PHYLIP
T11857 6098-6104 NN denotes format
T11858 6104-6106 , denotes ,
T11859 6106-6111 IN denotes after
T11861 6112-6117 WDT denotes which
T11862 6117-6119 , denotes ,
T11863 6119-6122 DT denotes the
T11864 6123-6132 NNS denotes distances
T11865 6133-6140 IN denotes between
T11866 6141-6149 NN denotes proteins
T11867 6150-6154 VBD denotes were
T11860 6155-6165 VBN denotes calculated
T11868 6166-6171 VBG denotes using
T11869 6172-6179 NNP denotes Poisson
T11870 6180-6190 NN denotes correction
T11871 6191-6194 CC denotes and
T11872 6195-6198 DT denotes the
T11874 6199-6207 JJ denotes Unrooted
T11873 6208-6213 NNS denotes trees
T11876 6214-6218 VBD denotes were
T11875 6219-6227 VBN denotes inferred
T11877 6228-6232 IN denotes with
T11878 6233-6236 DT denotes the
T11880 6237-6239 NN denotes NJ
T11879 6240-6246 NN denotes method
T11881 6247-6250 CC denotes and
T11882 6251-6255 RB denotes then
T11883 6256-6265 VBD denotes displayed
T11884 6266-6271 VBG denotes using
T11885 6272-6280 NNP denotes TreeView
T11886 6281-6282 -LRB- denotes [
T11887 6282-6284 CD denotes 39
T11888 6284-6285 -RRB- denotes ]
T11889 6285-6286 . denotes .
T11890 6286-6352 sentence denotes Bootstrap values above 700 for 1000 trials are shown at the node.
T11891 6287-6296 NN denotes Bootstrap
T11892 6297-6303 NNS denotes values
T11894 6304-6309 IN denotes above
T11895 6310-6313 CD denotes 700
T11896 6314-6317 IN denotes for
T11897 6318-6322 CD denotes 1000
T11898 6323-6329 NNS denotes trials
T11899 6330-6333 VBP denotes are
T11893 6334-6339 VBN denotes shown
T11900 6340-6342 IN denotes at
T11901 6343-6346 DT denotes the
T11902 6347-6351 NN denotes node
T11903 6351-6352 . denotes .
T11904 6352-6528 sentence denotes To validate the tree, the same sequence set was analyzed with tools in the PHYLIP package [40], using PRODIST followed by FITCH or NEIGNBOR and tree displaying using TreeView.
T11905 6353-6355 TO denotes To
T11906 6356-6364 VB denotes validate
T11908 6365-6368 DT denotes the
T11909 6369-6373 NN denotes tree
T11910 6373-6375 , denotes ,
T11911 6375-6378 DT denotes the
T11913 6379-6383 JJ denotes same
T11914 6384-6392 NN denotes sequence
T11912 6393-6396 NN denotes set
T11915 6397-6400 VBD denotes was
T11907 6401-6409 VBN denotes analyzed
T11916 6410-6414 IN denotes with
T11917 6415-6420 NNS denotes tools
T11918 6421-6423 IN denotes in
T11919 6424-6427 DT denotes the
T11921 6428-6434 NN denotes PHYLIP
T11920 6435-6442 NN denotes package
T11922 6443-6444 -LRB- denotes [
T11923 6444-6446 CD denotes 40
T11924 6446-6447 -RRB- denotes ]
T11925 6447-6449 , denotes ,
T11926 6449-6454 VBG denotes using
T11927 6455-6462 NN denotes PRODIST
T11928 6463-6471 VBN denotes followed
T11929 6472-6474 IN denotes by
T11930 6475-6480 NN denotes FITCH
T11931 6481-6483 CC denotes or
T11932 6484-6492 NN denotes NEIGNBOR
T11933 6493-6496 CC denotes and
T11934 6497-6501 NN denotes tree
T11935 6502-6512 VBG denotes displaying
T11936 6513-6518 VBG denotes using
T11937 6519-6527 NNP denotes TreeView
T11938 6527-6528 . denotes .
T11939 6528-6619 sentence denotes This additional method produced trees with essentially the same topology (data not shown).
T11940 6529-6533 DT denotes This
T11942 6534-6544 JJ denotes additional
T11941 6545-6551 NN denotes method
T11943 6552-6560 VBD denotes produced
T11944 6561-6566 NNS denotes trees
T11945 6567-6571 IN denotes with
T11946 6572-6583 RB denotes essentially
T11948 6584-6587 DT denotes the
T11949 6588-6592 JJ denotes same
T11947 6593-6601 NN denotes topology
T11950 6602-6603 -LRB- denotes (
T11952 6603-6607 NNS denotes data
T11953 6608-6611 RB denotes not
T11951 6612-6617 VBN denotes shown
T11954 6617-6618 -RRB- denotes )
T11955 6618-6619 . denotes .
T12120 6621-6623 FW denotes In
T12121 6624-6629 FW denotes vitro
T12122 6630-6641 NN denotes interaction
T12123 6642-6644 IN denotes of
T12124 6645-6650 NN denotes TACC2
T12125 6651-6654 CC denotes and
T12126 6655-6660 NN denotes RXRβ3
T12127 6660-6762 sentence denotes The TACC2 cDNA was cloned into GST fusion vector pGEX5X2 (Amersham Biosciences, Piscataway, NJ, USA).
T12128 6661-6664 DT denotes The
T12130 6665-6670 NN denotes TACC2
T12129 6671-6675 NN denotes cDNA
T12132 6676-6679 VBD denotes was
T12131 6680-6686 VBN denotes cloned
T12133 6687-6691 IN denotes into
T12134 6692-6695 NN denotes GST
T12136 6696-6702 NN denotes fusion
T12137 6703-6709 NN denotes vector
T12135 6710-6717 NN denotes pGEX5X2
T12138 6718-6719 -LRB- denotes (
T12140 6719-6727 NNP denotes Amersham
T12139 6728-6739 NNP denotes Biosciences
T12141 6739-6741 , denotes ,
T12142 6741-6751 NNP denotes Piscataway
T12143 6751-6753 , denotes ,
T12144 6753-6755 NNP denotes NJ
T12145 6755-6757 , denotes ,
T12146 6757-6760 NNP denotes USA
T12147 6760-6761 -RRB- denotes )
T12148 6761-6762 . denotes .
T12149 6762-6875 sentence denotes GST and GST-TACC2 proteins were expressed in E. coli BL21(DE3) plys "S" with 1 mM IPTG at 37°C shaker for 2 hrs.
T12150 6763-6766 NN denotes GST
T12152 6767-6770 CC denotes and
T12153 6771-6774 NN denotes GST
T12155 6774-6775 HYPH denotes -
T12154 6775-6780 NN denotes TACC2
T12151 6781-6789 NN denotes proteins
T12157 6790-6794 VBD denotes were
T12156 6795-6804 VBN denotes expressed
T12158 6805-6807 IN denotes in
T12159 6808-6810 NNP denotes E.
T12160 6811-6815 NNP denotes coli
T12162 6816-6820 NN denotes BL21
T12163 6820-6821 -LRB- denotes (
T12164 6821-6824 NN denotes DE3
T12165 6824-6825 -RRB- denotes )
T12166 6826-6830 NNS denotes plys
T12167 6831-6832 `` denotes "
T12161 6832-6833 NN denotes S
T12168 6833-6834 '' denotes "
T12169 6835-6839 IN denotes with
T12170 6840-6841 CD denotes 1
T12171 6842-6844 NN denotes mM
T12172 6845-6849 NN denotes IPTG
T12173 6850-6852 IN denotes at
T12174 6853-6855 CD denotes 37
T12175 6855-6857 NN denotes °C
T12176 6858-6864 NN denotes shaker
T12177 6865-6868 IN denotes for
T12178 6869-6870 CD denotes 2
T12179 6871-6874 NNS denotes hrs
T12180 6874-6875 . denotes .
T12181 6875-7025 sentence denotes Cells (50 ml) were harvested and resuspended in 5 ml of 20 mM Tris-HCl pH.8.0, 200 mM NaCl, 1 mM EDTA pH8.0, Protease inhibitor set III (Calbiochem).
T12182 6876-6881 NNS denotes Cells
T12184 6882-6883 -LRB- denotes (
T12186 6883-6885 CD denotes 50
T12185 6886-6888 NN denotes ml
T12187 6888-6889 -RRB- denotes )
T12188 6890-6894 VBD denotes were
T12183 6895-6904 VBN denotes harvested
T12189 6905-6908 CC denotes and
T12190 6909-6920 VBN denotes resuspended
T12191 6921-6923 IN denotes in
T12192 6924-6925 CD denotes 5
T12193 6926-6928 NN denotes ml
T12194 6929-6931 IN denotes of
T12195 6932-6934 CD denotes 20
T12196 6935-6937 NN denotes mM
T12198 6938-6942 NN denotes Tris
T12199 6942-6943 HYPH denotes -
T12197 6943-6946 NN denotes HCl
T12200 6947-6953 NN denotes pH.8.0
T12201 6953-6955 , denotes ,
T12202 6955-6958 CD denotes 200
T12203 6959-6961 NN denotes mM
T12204 6962-6966 NN denotes NaCl
T12205 6966-6968 , denotes ,
T12206 6968-6969 CD denotes 1
T12207 6970-6972 NN denotes mM
T12208 6973-6977 NN denotes EDTA
T12209 6978-6983 NN denotes pH8.0
T12210 6983-6985 , denotes ,
T12211 6985-6993 NN denotes Protease
T12212 6994-7003 NN denotes inhibitor
T12213 7004-7007 NN denotes set
T12214 7008-7011 CD denotes III
T12215 7012-7013 -LRB- denotes (
T12216 7013-7023 NNP denotes Calbiochem
T12217 7023-7024 -RRB- denotes )
T12218 7024-7025 . denotes .
T12219 7025-7128 sentence denotes The cells were lysed by sonication and lysate cleared by centrifugation at 7500 rpm at 4°C for 15 min.
T12220 7026-7029 DT denotes The
T12221 7030-7035 NNS denotes cells
T12223 7036-7040 VBD denotes were
T12222 7041-7046 VBN denotes lysed
T12224 7047-7049 IN denotes by
T12225 7050-7060 NN denotes sonication
T12226 7061-7064 CC denotes and
T12227 7065-7071 NN denotes lysate
T12228 7072-7079 VBN denotes cleared
T12229 7080-7082 IN denotes by
T12230 7083-7097 NN denotes centrifugation
T12231 7098-7100 IN denotes at
T12232 7101-7105 CD denotes 7500
T12233 7106-7109 NN denotes rpm
T12234 7110-7112 IN denotes at
T12235 7113-7114 CD denotes 4
T12236 7114-7116 NN denotes °C
T12237 7117-7120 IN denotes for
T12238 7121-7123 CD denotes 15
T12239 7124-7127 NN denotes min
T12240 7127-7128 . denotes .
T12241 7128-7259 sentence denotes The cleared lysate was immobilized on glutathione sepharose beads in 3 ml of 20 mM Tris-HCl pH.8.0, 200 mM NaCl, 1 mM EDTA pH8.0).
T12242 7129-7132 DT denotes The
T12244 7133-7140 JJ denotes cleared
T12243 7141-7147 NN denotes lysate
T12246 7148-7151 VBD denotes was
T12245 7152-7163 VBN denotes immobilized
T12247 7164-7166 IN denotes on
T12248 7167-7178 NN denotes glutathione
T12249 7179-7188 NN denotes sepharose
T12250 7189-7194 NNS denotes beads
T12251 7195-7197 IN denotes in
T12252 7198-7199 CD denotes 3
T12253 7200-7202 NN denotes ml
T12254 7203-7205 IN denotes of
T12255 7206-7208 CD denotes 20
T12256 7209-7211 NN denotes mM
T12258 7212-7216 NN denotes Tris
T12259 7216-7217 HYPH denotes -
T12257 7217-7220 NN denotes HCl
T12260 7221-7227 NN denotes pH.8.0
T12261 7227-7229 , denotes ,
T12262 7229-7232 CD denotes 200
T12263 7233-7235 NN denotes mM
T12264 7236-7240 NN denotes NaCl
T12265 7240-7242 , denotes ,
T12266 7242-7243 CD denotes 1
T12267 7244-7246 NN denotes mM
T12268 7247-7251 NN denotes EDTA
T12269 7252-7257 NN denotes pH8.0
T12270 7257-7258 -RRB- denotes )
T12271 7258-7259 . denotes .
T12272 7259-7500 sentence denotes RXRβ3 cDNA was cloned into pET 28C(+) (Invitrogen, Carlsbad, CA, USA) and protein synthesized by TNT quick coupled transcription/translation system kit (Promega) and radiolabeled with 35S methionine according to manufacturer's instructions.
T12273 7260-7265 NN denotes RXRβ3
T12274 7266-7270 NN denotes cDNA
T12276 7271-7274 VBD denotes was
T12275 7275-7281 VBN denotes cloned
T12277 7282-7286 IN denotes into
T12278 7287-7290 NN denotes pET
T12279 7291-7294 NN denotes 28C
T12280 7294-7295 -LRB- denotes (
T12281 7295-7296 SYM denotes +
T12282 7296-7297 -RRB- denotes )
T12283 7298-7299 -LRB- denotes (
T12284 7299-7309 NNP denotes Invitrogen
T12285 7309-7311 , denotes ,
T12286 7311-7319 NNP denotes Carlsbad
T12287 7319-7321 , denotes ,
T12288 7321-7323 NNP denotes CA
T12289 7323-7325 , denotes ,
T12290 7325-7328 NNP denotes USA
T12291 7328-7329 -RRB- denotes )
T12292 7330-7333 CC denotes and
T12293 7334-7341 NN denotes protein
T12294 7342-7353 VBN denotes synthesized
T12295 7354-7356 IN denotes by
T12296 7357-7360 NN denotes TNT
T12298 7361-7366 RB denotes quick
T12299 7367-7374 VBN denotes coupled
T12300 7375-7388 NN denotes transcription
T12302 7388-7389 HYPH denotes /
T12301 7389-7400 NN denotes translation
T12303 7401-7407 NN denotes system
T12297 7408-7411 NN denotes kit
T12304 7412-7413 -LRB- denotes (
T12305 7413-7420 NNP denotes Promega
T12306 7420-7421 -RRB- denotes )
T12307 7422-7425 CC denotes and
T12308 7426-7438 VBN denotes radiolabeled
T12309 7439-7443 IN denotes with
T12310 7444-7447 NN denotes 35S
T12311 7448-7458 NN denotes methionine
T12312 7459-7468 VBG denotes according
T12313 7469-7471 IN denotes to
T12314 7472-7484 NN denotes manufacturer
T12316 7484-7486 POS denotes 's
T12315 7487-7499 NNS denotes instructions
T12317 7499-7500 . denotes .
T12318 7500-7674 sentence denotes 100 μl of in vitro translated RXRβ3 protein in 1 ml of 20 mM Tris-HCl pH.8.0, 200 mM NaCl, 1 mM EDTA pH8.0 was incubated at 4°C with immobilized GST-TACC2 or GST for 90 min.
T12319 7501-7504 CD denotes 100
T12320 7505-7507 NN denotes μl
T12322 7508-7510 IN denotes of
T12323 7511-7513 FW denotes in
T12324 7514-7519 FW denotes vitro
T12325 7520-7530 VBN denotes translated
T12327 7531-7536 NN denotes RXRβ3
T12326 7537-7544 NN denotes protein
T12328 7545-7547 IN denotes in
T12329 7548-7549 CD denotes 1
T12330 7550-7552 NN denotes ml
T12331 7553-7555 IN denotes of
T12332 7556-7558 CD denotes 20
T12333 7559-7561 NN denotes mM
T12335 7562-7566 NN denotes Tris
T12336 7566-7567 HYPH denotes -
T12334 7567-7570 NN denotes HCl
T12337 7571-7577 NN denotes pH.8.0
T12338 7577-7579 , denotes ,
T12339 7579-7582 CD denotes 200
T12340 7583-7585 NN denotes mM
T12341 7586-7590 NN denotes NaCl
T12342 7590-7592 , denotes ,
T12343 7592-7593 CD denotes 1
T12344 7594-7596 NN denotes mM
T12345 7597-7601 NN denotes EDTA
T12346 7602-7607 NN denotes pH8.0
T12347 7608-7611 VBD denotes was
T12321 7612-7621 VBN denotes incubated
T12348 7622-7624 IN denotes at
T12349 7625-7626 CD denotes 4
T12350 7626-7628 NN denotes °C
T12351 7629-7633 IN denotes with
T12352 7634-7645 VBN denotes immobilized
T12354 7646-7649 NN denotes GST
T12355 7649-7650 HYPH denotes -
T12353 7650-7655 NN denotes TACC2
T12356 7656-7658 CC denotes or
T12357 7659-7662 NN denotes GST
T12358 7663-7666 IN denotes for
T12359 7667-7669 CD denotes 90
T12360 7670-7673 NN denotes min
T12361 7673-7674 . denotes .
T12362 7674-7781 sentence denotes Unbound RXRβ3 was removed by washing three times with 20 mM Tris-HCl pH.8.0, 200 mM NaCl, 1 mM EDTA pH8.0.
T12363 7675-7682 JJ denotes Unbound
T12364 7683-7688 NN denotes RXRβ3
T12366 7689-7692 VBD denotes was
T12365 7693-7700 VBN denotes removed
T12367 7701-7703 IN denotes by
T12368 7704-7711 VBG denotes washing
T12369 7712-7717 CD denotes three
T12370 7718-7723 NNS denotes times
T12371 7724-7728 IN denotes with
T12372 7729-7731 CD denotes 20
T12373 7732-7734 NN denotes mM
T12375 7735-7739 NN denotes Tris
T12376 7739-7740 HYPH denotes -
T12374 7740-7743 NN denotes HCl
T12377 7744-7750 NN denotes pH.8.0
T12378 7750-7752 , denotes ,
T12379 7752-7755 CD denotes 200
T12380 7756-7758 NN denotes mM
T12381 7759-7763 NN denotes NaCl
T12382 7763-7765 , denotes ,
T12383 7765-7766 CD denotes 1
T12384 7767-7769 NN denotes mM
T12385 7770-7774 NN denotes EDTA
T12386 7775-7780 NN denotes pH8.0
T12387 7780-7781 . denotes .
T12388 7781-7925 sentence denotes Bound proteins were eluted from the beads at room temperature for 10 min in elution buffer (100 mM Tris HCl, pH8.0, 20 mM reduced glutathione).
T12389 7782-7787 NN denotes Bound
T12390 7788-7796 NN denotes proteins
T12392 7797-7801 VBD denotes were
T12391 7802-7808 VBN denotes eluted
T12393 7809-7813 IN denotes from
T12394 7814-7817 DT denotes the
T12395 7818-7823 NNS denotes beads
T12396 7824-7826 IN denotes at
T12397 7827-7831 NN denotes room
T12398 7832-7843 NN denotes temperature
T12399 7844-7847 IN denotes for
T12400 7848-7850 CD denotes 10
T12401 7851-7854 NN denotes min
T12402 7855-7857 IN denotes in
T12403 7858-7865 NN denotes elution
T12404 7866-7872 NN denotes buffer
T12405 7873-7874 -LRB- denotes (
T12407 7874-7877 CD denotes 100
T12408 7878-7880 NN denotes mM
T12409 7881-7885 NN denotes Tris
T12406 7886-7889 NN denotes HCl
T12410 7889-7891 , denotes ,
T12411 7891-7896 NN denotes pH8.0
T12412 7896-7898 , denotes ,
T12413 7898-7900 CD denotes 20
T12414 7901-7903 NN denotes mM
T12416 7904-7911 VBN denotes reduced
T12415 7912-7923 NN denotes glutathione
T12417 7923-7924 -RRB- denotes )
T12418 7924-7925 . denotes .
T12419 7925-7984 sentence denotes The proteins were analyzed on 12% SDS polyacrylamide gels.
T12420 7926-7929 DT denotes The
T12421 7930-7938 NN denotes proteins
T12423 7939-7943 VBD denotes were
T12422 7944-7952 VBN denotes analyzed
T12424 7953-7955 IN denotes on
T12425 7956-7958 CD denotes 12
T12426 7958-7959 NN denotes %
T12428 7960-7963 NN denotes SDS
T12429 7964-7978 NN denotes polyacrylamide
T12427 7979-7983 NNS denotes gels
T12430 7983-7984 . denotes .
T12431 7984-8054 sentence denotes Coomassie blue staining verified equal loading of GST fusion protein.
T12432 7985-7994 NNP denotes Coomassie
T12434 7995-7999 JJ denotes blue
T12433 8000-8008 NN denotes staining
T12435 8009-8017 VBD denotes verified
T12436 8018-8023 JJ denotes equal
T12437 8024-8031 NN denotes loading
T12438 8032-8034 IN denotes of
T12439 8035-8038 NN denotes GST
T12441 8039-8045 NN denotes fusion
T12440 8046-8053 NN denotes protein
T12442 8053-8054 . denotes .
T12443 8054-8088 sentence denotes Dried gels were autoradiographed.
T12444 8055-8060 VBN denotes Dried
T12445 8061-8065 NNS denotes gels
T12447 8066-8070 VBD denotes were
T12446 8071-8087 VBN denotes autoradiographed
T12448 8087-8088 . denotes .
R6478 T10099 T10098 cc and,Compilation
R6479 T10100 T10098 conj assembly,Compilation
R6480 T10101 T10098 prep of,Compilation
R6481 T10102 T10103 advmod previously,uncharacterized
R6482 T10103 T10104 amod uncharacterized,cDNAs
R6483 T10104 T10101 pobj cDNAs,of
R6484 T10105 T10104 compound TACC,cDNAs
R6485 T10106 T10104 cc and,cDNAs
R6486 T10107 T10104 conj genes,cDNAs
R6487 T10109 T10110 amod Corresponding,sequences
R6488 T10110 T10112 nsubjpass sequences,identified
R6489 T10111 T10110 amod orthologous,sequences
R6490 T10113 T10110 prep for,sequences
R6491 T10114 T10115 nmod TACC,families
R6492 T10115 T10113 pobj families,for
R6493 T10116 T10114 punct ", ",TACC
R6494 T10117 T10114 conj RHAMM,TACC
R6495 T10118 T10117 punct ", ",RHAMM
R6496 T10119 T10117 conj KLP,RHAMM
R6497 T10120 T10119 punct ", ",KLP
R6498 T10121 T10119 conj KIF,KLP
R6499 T10122 T10121 punct ", ",KIF
R6500 T10123 T10121 conj TPM,KIF
R6501 T10124 T10123 cc and,TPM
R6502 T10125 T10123 conj keratins,TPM
R6503 T10126 T10112 auxpass were,identified
R6504 T10127 T10112 advmod initially,identified
R6505 T10128 T10112 advcl using,identified
R6506 T10129 T10130 det the,program
R6507 T10130 T10128 dobj program,using
R6508 T10131 T10130 compound TBLASTN,program
R6509 T10132 T10133 punct [,36
R6510 T10133 T10128 parataxis 36,using
R6511 T10134 T10133 punct ],36
R6512 T10135 T10136 aux to,search
R6513 T10136 T10128 advcl search,using
R6514 T10137 T10138 det the,databases
R6515 T10138 T10136 dobj databases,search
R6516 T10139 T10138 amod published,databases
R6517 T10140 T10138 amod genomic,databases
R6518 T10141 T10140 cc and,genomic
R6519 T10142 T10140 conj cDNA,genomic
R6520 T10143 T10112 punct .,identified
R6521 T10145 T10146 prep For,produced
R6522 T10147 T10148 compound Takifugu,rubripes
R6523 T10148 T10145 pobj rubripes,For
R6524 T10149 T10146 punct ", ",produced
R6525 T10150 T10151 compound gene,predictions
R6526 T10151 T10146 nsubjpass predictions,produced
R6527 T10152 T10146 auxpass were,produced
R6528 T10153 T10146 agent by,produced
R6529 T10154 T10155 det the,pipeline
R6530 T10155 T10153 pobj pipeline,by
R6531 T10156 T10155 nmod Ensembl,pipeline
R6532 T10157 T10155 amod automated,pipeline
R6533 T10158 T10159 punct [,37
R6534 T10159 T10155 parataxis 37,pipeline
R6535 T10160 T10159 punct ],37
R6536 T10161 T10155 cc and,pipeline
R6537 T10162 T10163 det the,server
R6538 T10163 T10155 conj server,pipeline
R6539 T10164 T10163 compound JGI,server
R6540 T10165 T10163 compound blast,server
R6541 T10166 T10146 punct .,produced
R6542 T10168 T10169 compound DNA,sequences
R6543 T10169 T10170 nsubj sequences,extracted
R6544 T10171 T10169 acl covering,sequences
R6545 T10172 T10173 det the,regions
R6546 T10173 T10171 dobj regions,covering
R6547 T10174 T10173 compound homology,regions
R6548 T10175 T10170 aux were,extracted
R6549 T10176 T10170 cc and,extracted
R6550 T10177 T10170 conj analyzed,extracted
R6551 T10178 T10177 prep by,analyzed
R6552 T10179 T10178 pobj Genscan,by
R6553 T10180 T10181 aux to,obtain
R6554 T10181 T10170 advcl obtain,extracted
R6555 T10182 T10183 amod potential,exons
R6556 T10183 T10181 dobj exons,obtain
R6557 T10184 T10170 punct .,extracted
R6558 T10186 T10187 prep In,added
R6559 T10188 T10189 det some,cases
R6560 T10189 T10186 pobj cases,In
R6561 T10190 T10187 punct ", ",added
R6562 T10191 T10187 nsubjpass exons,added
R6563 T10192 T10187 auxpass were,added
R6564 T10193 T10187 cc or,added
R6565 T10194 T10187 conj modified,added
R6566 T10195 T10194 prep based,modified
R6567 T10196 T10195 prep on,based
R6568 T10197 T10198 det the,similarity
R6569 T10198 T10196 pobj similarity,on
R6570 T10199 T10198 amod best,similarity
R6571 T10200 T10198 prep of,similarity
R6572 T10201 T10202 amod translated,peptides
R6573 T10202 T10200 pobj peptides,of
R6574 T10203 T10198 prep to,similarity
R6575 T10204 T10205 det the,proteins
R6576 T10205 T10203 pobj proteins,to
R6577 T10206 T10205 amod corresponding,proteins
R6578 T10207 T10205 nmod mouse,proteins
R6579 T10208 T10207 cc and,mouse
R6580 T10209 T10207 conj human,mouse
R6581 T10210 T10187 punct .,added
R6582 T10212 T10213 prep For,used
R6583 T10214 T10212 pobj regions,For
R6584 T10215 T10214 prep with,regions
R6585 T10216 T10217 amod low,similarity
R6586 T10217 T10215 pobj similarity,with
R6587 T10218 T10217 compound sequence,similarity
R6588 T10219 T10213 punct ", ",used
R6589 T10220 T10221 amod genomic,sequences
R6590 T10221 T10213 nsubjpass sequences,used
R6591 T10222 T10221 prep from,sequences
R6592 T10223 T10224 det the,pufferfish
R6593 T10224 T10222 pobj pufferfish,from
R6594 T10225 T10226 amod fresh,water
R6595 T10226 T10224 compound water,pufferfish
R6596 T10227 T10224 punct ", ",pufferfish
R6597 T10228 T10229 compound Tetraodon,nigroviridis
R6598 T10229 T10224 appos nigroviridis,pufferfish
R6599 T10230 T10213 auxpass were,used
R6600 T10231 T10213 prep as,used
R6601 T10232 T10233 amod additional,means
R6602 T10233 T10231 pobj means,as
R6603 T10234 T10235 aux to,verify
R6604 T10235 T10233 advcl verify,means
R6605 T10236 T10237 det the,exons
R6606 T10237 T10235 dobj exons,verify
R6607 T10238 T10237 amod predicted,exons
R6608 T10239 T10213 punct .,used
R6609 T10241 T10242 prep Due,obtained
R6610 T10243 T10241 pcomp to,Due
R6611 T10244 T10245 det the,variability
R6612 T10245 T10241 pobj variability,Due
R6613 T10246 T10245 prep of,variability
R6614 T10247 T10248 det the,region
R6615 T10248 T10246 pobj region,of
R6616 T10249 T10248 amod central,region
R6617 T10250 T10248 prep of,region
R6618 T10251 T10252 compound vertebrate,cDNAs
R6619 T10252 T10250 pobj cDNAs,of
R6620 T10253 T10252 compound TACC3,cDNAs
R6621 T10254 T10255 punct (,see
R6622 T10255 T10245 parataxis see,variability
R6623 T10256 T10255 dobj text,see
R6624 T10257 T10255 punct ),see
R6625 T10258 T10242 punct ", ",obtained
R6626 T10259 T10260 aux to,confirm
R6627 T10260 T10242 advcl confirm,obtained
R6628 T10261 T10260 advmod further,confirm
R6629 T10262 T10260 dobj prediction,confirm
R6630 T10263 T10262 prep of,prediction
R6631 T10264 T10265 det the,rubripes
R6632 T10265 T10263 pobj rubripes,of
R6633 T10266 T10265 compound Takifugu,rubripes
R6634 T10267 T10265 appos TACC3,rubripes
R6635 T10268 T10242 punct ", ",obtained
R6636 T10269 T10270 amod full,length
R6637 T10270 T10271 compound length,cDNAs
R6638 T10271 T10242 nsubjpass cDNAs,obtained
R6639 T10272 T10271 acl corresponding,cDNAs
R6640 T10273 T10272 prep to,corresponding
R6641 T10274 T10275 det the,TACC3
R6642 T10275 T10273 pobj TACC3,to
R6643 T10276 T10275 compound Danio,TACC3
R6644 T10277 T10275 compound rerio,TACC3
R6645 T10278 T10279 punct (,2639991
R6646 T10279 T10275 parataxis 2639991,TACC3
R6647 T10280 T10279 nmod IMAGE,2639991
R6648 T10281 T10279 nmod clones,2639991
R6649 T10282 T10279 punct ", ",2639991
R6650 T10283 T10279 conj 2640369,2639991
R6651 T10284 T10283 cc and,2640369
R6652 T10285 T10283 conj 3724452,2640369
R6653 T10286 T10279 punct ),2639991
R6654 T10287 T10242 auxpass were,obtained
R6655 T10288 T10242 advmod also,obtained
R6656 T10289 T10242 prep from,obtained
R6657 T10290 T10289 pobj A.T.C.C.,from
R6658 T10291 T10242 cc and,obtained
R6659 T10292 T10293 advmod fully,sequenced
R6660 T10293 T10242 conj sequenced,obtained
R6661 T10294 T10242 punct .,obtained
R6662 T10296 T10297 amod Potential,segments
R6663 T10297 T10300 nsubjpass segments,identified
R6664 T10298 T10297 amod paralogous,segments
R6665 T10299 T10297 amod chromosomal,segments
R6666 T10301 T10297 cc and,segments
R6667 T10302 T10297 conj scaffold,segments
R6668 T10303 T10300 auxpass were,identified
R6669 T10304 T10300 prep by,identified
R6670 T10305 T10304 pcomp searching,by
R6671 T10306 T10307 det the,databases
R6672 T10307 T10305 dobj databases,searching
R6673 T10308 T10307 amod public,databases
R6674 T10309 T10307 acl deposited,databases
R6675 T10310 T10309 prep at,deposited
R6676 T10311 T10310 pobj NCBI,at
R6677 T10312 T10310 cc and,at
R6678 T10313 T10310 conj at,at
R6679 T10314 T10315 det the,Project
R6680 T10315 T10313 pobj Project,at
R6681 T10316 T10317 compound Human,Genome
R6682 T10317 T10315 compound Genome,Project
R6683 T10318 T10315 compound Mapping,Project
R6684 T10319 T10315 punct ", ",Project
R6685 T10320 T10315 npadvmod Cambridge,Project
R6686 T10321 T10320 npadvmod UK,Cambridge
R6687 T10322 T10300 punct .,identified
R6688 T10440 T10439 prep of,Cloning
R6689 T10441 T10442 compound vertebrate,TACC
R6690 T10442 T10443 compound TACC,cDNAs
R6691 T10443 T10440 pobj cDNAs,of
R6692 T10445 T10446 det The,TACC3
R6693 T10446 T10448 nsubjpass TACC3,cloned
R6694 T10447 T10446 compound rabbit,TACC3
R6695 T10449 T10448 auxpass was,cloned
R6696 T10450 T10448 prep by,cloned
R6697 T10451 T10452 compound rt,PCR
R6698 T10452 T10450 pobj PCR,by
R6699 T10453 T10452 punct -,PCR
R6700 T10454 T10448 advcl using,cloned
R6701 T10455 T10456 det the,primer
R6702 T10456 T10454 dobj primer,using
R6703 T10457 T10458 punct """",specific
R6704 T10458 T10456 amod specific,primer
R6705 T10459 T10458 npadvmod TACC4,specific
R6706 T10460 T10458 punct """",specific
R6707 T10461 T10456 appos T4RACE2,primer
R6708 T10462 T10463 punct [,9
R6709 T10463 T10456 parataxis 9,primer
R6710 T10464 T10463 punct ],9
R6711 T10465 T10466 punct (,cccgaactgctccaggtaatcgatctc
R6712 T10466 T10456 parataxis cccgaactgctccaggtaatcgatctc,primer
R6713 T10467 T10466 nummod 5,cccgaactgctccaggtaatcgatctc
R6714 T10468 T10467 punct ',5
R6715 T10469 T10466 punct -,cccgaactgctccaggtaatcgatctc
R6716 T10470 T10466 punct -,cccgaactgctccaggtaatcgatctc
R6717 T10471 T10466 nummod 3,cccgaactgctccaggtaatcgatctc
R6718 T10472 T10466 punct ',cccgaactgctccaggtaatcgatctc
R6719 T10473 T10466 punct ),cccgaactgctccaggtaatcgatctc
R6720 T10474 T10456 cc and,primer
R6721 T10475 T10476 det a,primer
R6722 T10476 T10456 conj primer,primer
R6723 T10477 T10476 compound consensus,primer
R6724 T10478 T10476 punct ", ",primer
R6725 T10479 T10476 appos T3con2,primer
R6726 T10480 T10476 punct ", ",primer
R6727 T10481 T10476 acl designed,primer
R6728 T10482 T10481 prep to,designed
R6729 T10483 T10484 det the,region
R6730 T10484 T10482 pobj region,to
R6731 T10485 T10484 acl encompassing,region
R6732 T10486 T10487 det the,initiator
R6733 T10487 T10485 dobj initiator,encompassing
R6734 T10488 T10487 compound vertebrate,initiator
R6735 T10489 T10487 compound TACC3,initiator
R6736 T10490 T10487 appos methionine,initiator
R6737 T10491 T10492 punct (,tatgagtctgcaggtcttaaacgac
R6738 T10492 T10487 parataxis tatgagtctgcaggtcttaaacgac,initiator
R6739 T10493 T10492 nummod 5,tatgagtctgcaggtcttaaacgac
R6740 T10494 T10493 punct ',5
R6741 T10495 T10492 punct -,tatgagtctgcaggtcttaaacgac
R6742 T10496 T10492 punct -,tatgagtctgcaggtcttaaacgac
R6743 T10497 T10492 nummod 3,tatgagtctgcaggtcttaaacgac
R6744 T10498 T10492 punct ',tatgagtctgcaggtcttaaacgac
R6745 T10499 T10492 punct ),tatgagtctgcaggtcttaaacgac
R6746 T10500 T10448 punct .,cloned
R6747 T10502 T10503 prep For,based
R6748 T10504 T10502 pcomp cloning,For
R6749 T10505 T10506 det the,cDNA
R6750 T10506 T10504 dobj cDNA,cloning
R6751 T10507 T10508 compound mouse,Tacc1X
R6752 T10508 T10506 compound Tacc1X,cDNA
R6753 T10509 T10503 punct ", ",based
R6754 T10510 T10511 det the,primers
R6755 T10511 T10503 nsubjpass primers,based
R6756 T10512 T10511 acl used,primers
R6757 T10513 T10503 auxpass were,based
R6758 T10514 T10503 prep upon,based
R6759 T10515 T10516 det the,sequence
R6760 T10516 T10514 pobj sequence,upon
R6761 T10517 T10516 amod genomic,sequence
R6762 T10518 T10516 acl reported,sequence
R6763 T10519 T10516 punct ", ",sequence
R6764 T10520 T10516 cc and,sequence
R6765 T10521 T10522 det the,sequence
R6766 T10522 T10516 conj sequence,sequence
R6767 T10523 T10522 prep of,sequence
R6768 T10524 T10525 det the,clone
R6769 T10525 T10523 pobj clone,of
R6770 T10526 T10525 compound IMAGE,clone
R6771 T10527 T10525 compound cDNA,clone
R6772 T10528 T10525 appos 4933429K08,clone
R6773 T10529 T10522 punct : ,sequence
R6774 T10530 T10522 appos T1XF,sequence
R6775 T10531 T10532 punct (,ccatgttcagtcattggcaggtc
R6776 T10532 T10530 parataxis ccatgttcagtcattggcaggtc,T1XF
R6777 T10533 T10532 nummod 5,ccatgttcagtcattggcaggtc
R6778 T10534 T10533 punct ',5
R6779 T10535 T10532 punct -,ccatgttcagtcattggcaggtc
R6780 T10536 T10532 punct -,ccatgttcagtcattggcaggtc
R6781 T10537 T10532 nummod 3,ccatgttcagtcattggcaggtc
R6782 T10538 T10532 punct ',ccatgttcagtcattggcaggtc
R6783 T10539 T10532 punct ),ccatgttcagtcattggcaggtc
R6784 T10540 T10530 punct ", ",T1XF
R6785 T10541 T10530 conj T1XF2,T1XF
R6786 T10542 T10543 punct (,ctgcagaaccaacagttcaag
R6787 T10543 T10541 parataxis ctgcagaaccaacagttcaag,T1XF2
R6788 T10544 T10543 nummod 5,ctgcagaaccaacagttcaag
R6789 T10545 T10544 punct ',5
R6790 T10546 T10543 punct -,ctgcagaaccaacagttcaag
R6791 T10547 T10543 punct -,ctgcagaaccaacagttcaag
R6792 T10548 T10543 nummod 3,ctgcagaaccaacagttcaag
R6793 T10549 T10543 punct ',ctgcagaaccaacagttcaag
R6794 T10550 T10543 punct ),ctgcagaaccaacagttcaag
R6795 T10551 T10541 punct ", ",T1XF2
R6796 T10552 T10541 conj T1XR1,T1XF2
R6797 T10553 T10554 punct (,agatctgtgacatcacagctc
R6798 T10554 T10552 parataxis agatctgtgacatcacagctc,T1XR1
R6799 T10555 T10554 nummod 5,agatctgtgacatcacagctc
R6800 T10556 T10555 punct ',5
R6801 T10557 T10554 punct -,agatctgtgacatcacagctc
R6802 T10558 T10554 punct -,agatctgtgacatcacagctc
R6803 T10559 T10554 nummod 3,agatctgtgacatcacagctc
R6804 T10560 T10554 punct ',agatctgtgacatcacagctc
R6805 T10561 T10554 punct ),agatctgtgacatcacagctc
R6806 T10562 T10552 punct ", ",T1XR1
R6807 T10563 T10552 conj T1XR2,T1XR1
R6808 T10564 T10565 punct (,ctcgagtcagttagtcttatccagctt
R6809 T10565 T10563 parataxis ctcgagtcagttagtcttatccagctt,T1XR2
R6810 T10566 T10565 nummod 5,ctcgagtcagttagtcttatccagctt
R6811 T10567 T10566 punct ',5
R6812 T10568 T10565 punct -,ctcgagtcagttagtcttatccagctt
R6813 T10569 T10565 punct -,ctcgagtcagttagtcttatccagctt
R6814 T10570 T10565 nummod 3,ctcgagtcagttagtcttatccagctt
R6815 T10571 T10565 punct ',ctcgagtcagttagtcttatccagctt
R6816 T10572 T10565 punct ),ctcgagtcagttagtcttatccagctt
R6817 T10573 T10563 punct ", ",T1XR2
R6818 T10574 T10563 conj BB617F,T1XR2
R6819 T10575 T10576 punct (,accaccaacttgagtacctg
R6820 T10576 T10574 parataxis accaccaacttgagtacctg,BB617F
R6821 T10577 T10576 nummod 5,accaccaacttgagtacctg
R6822 T10578 T10577 punct ',5
R6823 T10579 T10576 punct -,accaccaacttgagtacctg
R6824 T10580 T10576 punct -,accaccaacttgagtacctg
R6825 T10581 T10576 nummod 3,accaccaacttgagtacctg
R6826 T10582 T10576 punct ',accaccaacttgagtacctg
R6827 T10583 T10576 punct ),accaccaacttgagtacctg
R6828 T10584 T10574 cc and,BB617F
R6829 T10585 T10574 conj BB617R,BB617F
R6830 T10586 T10587 punct (,gtatcttgaactgttggttctg
R6831 T10587 T10585 parataxis gtatcttgaactgttggttctg,BB617R
R6832 T10588 T10587 nummod 5,gtatcttgaactgttggttctg
R6833 T10589 T10588 punct ',5
R6834 T10590 T10587 punct -,gtatcttgaactgttggttctg
R6835 T10591 T10587 punct -,gtatcttgaactgttggttctg
R6836 T10592 T10587 nummod 3,gtatcttgaactgttggttctg
R6837 T10593 T10587 punct ',gtatcttgaactgttggttctg
R6838 T10594 T10587 punct ),gtatcttgaactgttggttctg
R6839 T10595 T10503 punct .,based
R6840 T10597 T10598 prep For,located
R6841 T10599 T10597 pobj analysis,For
R6842 T10600 T10599 prep of,analysis
R6843 T10601 T10602 compound TACC1,variants
R6844 T10602 T10600 pobj variants,of
R6845 T10603 T10602 compound splice,variants
R6846 T10604 T10598 punct ", ",located
R6847 T10605 T10606 det the,primers
R6848 T10606 T10598 nsubjpass primers,located
R6849 T10607 T10606 amod forward,primers
R6850 T10608 T10606 acl used,primers
R6851 T10609 T10598 auxpass were,located
R6852 T10610 T10598 prep in,located
R6853 T10611 T10610 pobj exon,in
R6854 T10612 T10611 nummod 1b,exon
R6855 T10613 T10611 punct : ,exon
R6856 T10614 T10615 dep EF,gagagatgcgaaatcagcg
R6857 T10615 T10611 parataxis gagagatgcgaaatcagcg,exon
R6858 T10616 T10615 punct (,gagagatgcgaaatcagcg
R6859 T10617 T10615 nummod 5,gagagatgcgaaatcagcg
R6860 T10618 T10617 punct ',5
R6861 T10619 T10615 punct -,gagagatgcgaaatcagcg
R6862 T10620 T10615 punct -,gagagatgcgaaatcagcg
R6863 T10621 T10615 nummod 3,gagagatgcgaaatcagcg
R6864 T10622 T10615 punct ',gagagatgcgaaatcagcg
R6865 T10623 T10615 punct ),gagagatgcgaaatcagcg
R6866 T10624 T10611 punct ", ",exon
R6867 T10625 T10611 appos Exon,exon
R6868 T10626 T10625 nummod 1d,Exon
R6869 T10627 T10625 punct : ,Exon
R6870 T10628 T10629 dep X3F,agtcaaagaaggcatctgcag
R6871 T10629 T10625 parataxis agtcaaagaaggcatctgcag,Exon
R6872 T10630 T10629 punct (,agtcaaagaaggcatctgcag
R6873 T10631 T10629 nummod 5,agtcaaagaaggcatctgcag
R6874 T10632 T10631 punct ',5
R6875 T10633 T10629 punct -,agtcaaagaaggcatctgcag
R6876 T10634 T10629 punct -,agtcaaagaaggcatctgcag
R6877 T10635 T10629 nummod 3,agtcaaagaaggcatctgcag
R6878 T10636 T10629 punct ',agtcaaagaaggcatctgcag
R6879 T10637 T10629 punct ),agtcaaagaaggcatctgcag
R6880 T10638 T10611 punct ", ",exon
R6881 T10639 T10611 appos Exon,exon
R6882 T10640 T10639 nummod 1a,Exon
R6883 T10641 T10639 punct : ,Exon
R6884 T10642 T10643 dep 1DF,ccaagttctgcgccatggg
R6885 T10643 T10639 parataxis ccaagttctgcgccatggg,Exon
R6886 T10644 T10643 punct (,ccaagttctgcgccatggg
R6887 T10645 T10643 nummod 5,ccaagttctgcgccatggg
R6888 T10646 T10645 punct ',5
R6889 T10647 T10643 punct -,ccaagttctgcgccatggg
R6890 T10648 T10643 punct -,ccaagttctgcgccatggg
R6891 T10649 T10643 nummod 3,ccaagttctgcgccatggg
R6892 T10650 T10643 punct ',ccaagttctgcgccatggg
R6893 T10651 T10643 punct ),ccaagttctgcgccatggg
R6894 T10652 T10598 punct .,located
R6895 T10654 T10655 det The,primers
R6896 T10655 T10657 nsubj primers,were
R6897 T10656 T10655 amod reverse,primers
R6898 T10658 T10655 acl used,primers
R6899 T10659 T10657 punct : ,were
R6900 T10660 T10657 attr Exon,were
R6901 T10661 T10660 nummod 1,Exon
R6902 T10662 T10660 punct : ,Exon
R6903 T10663 T10664 dep X1R,ggatttggtctcggcttgcgaatc
R6904 T10664 T10660 parataxis ggatttggtctcggcttgcgaatc,Exon
R6905 T10665 T10664 punct (,ggatttggtctcggcttgcgaatc
R6906 T10666 T10664 nummod 5,ggatttggtctcggcttgcgaatc
R6907 T10667 T10666 punct ',5
R6908 T10668 T10664 punct -,ggatttggtctcggcttgcgaatc
R6909 T10669 T10664 punct -,ggatttggtctcggcttgcgaatc
R6910 T10670 T10664 nummod 3,ggatttggtctcggcttgcgaatc
R6911 T10671 T10664 punct ',ggatttggtctcggcttgcgaatc
R6912 T10672 T10664 punct ),ggatttggtctcggcttgcgaatc
R6913 T10673 T10660 punct ", ",Exon
R6914 T10674 T10660 appos Exon,Exon
R6915 T10675 T10674 nummod 2,Exon
R6916 T10676 T10674 punct : ,Exon
R6917 T10677 T10678 dep BX647R,cttgtgattcttggcttttgg
R6918 T10678 T10674 parataxis cttgtgattcttggcttttgg,Exon
R6919 T10679 T10678 punct (,cttgtgattcttggcttttgg
R6920 T10680 T10678 nummod 5,cttgtgattcttggcttttgg
R6921 T10681 T10680 punct ',5
R6922 T10682 T10678 punct -,cttgtgattcttggcttttgg
R6923 T10683 T10678 punct -,cttgtgattcttggcttttgg
R6924 T10684 T10678 nummod 3,cttgtgattcttggcttttgg
R6925 T10685 T10678 punct ',cttgtgattcttggcttttgg
R6926 T10686 T10678 punct ),cttgtgattcttggcttttgg
R6927 T10687 T10660 punct ", ",Exon
R6928 T10688 T10660 appos Exon,Exon
R6929 T10689 T10688 nummod 3,Exon
R6930 T10690 T10688 punct : ,Exon
R6931 T10691 T10692 dep 6SPR,gtcatcgcctcgtcctggagggc
R6932 T10692 T10688 parataxis gtcatcgcctcgtcctggagggc,Exon
R6933 T10693 T10692 punct (,gtcatcgcctcgtcctggagggc
R6934 T10694 T10692 nummod 5,gtcatcgcctcgtcctggagggc
R6935 T10695 T10694 punct ',5
R6936 T10696 T10692 punct -,gtcatcgcctcgtcctggagggc
R6937 T10697 T10692 punct -,gtcatcgcctcgtcctggagggc
R6938 T10698 T10692 nummod 3,gtcatcgcctcgtcctggagggc
R6939 T10699 T10692 punct ',gtcatcgcctcgtcctggagggc
R6940 T10700 T10692 punct ),gtcatcgcctcgtcctggagggc
R6941 T10701 T10660 punct ", ",Exon
R6942 T10702 T10703 compound Exon,4a
R6943 T10703 T10660 appos 4a,Exon
R6944 T10704 T10703 punct : ,4a
R6945 T10705 T10706 dep 1DR,aatttcacttgttcagtagtc
R6946 T10706 T10703 parataxis aatttcacttgttcagtagtc,4a
R6947 T10707 T10706 punct (,aatttcacttgttcagtagtc
R6948 T10708 T10706 nummod 5,aatttcacttgttcagtagtc
R6949 T10709 T10708 punct ',5
R6950 T10710 T10706 punct -,aatttcacttgttcagtagtc
R6951 T10711 T10706 punct -,aatttcacttgttcagtagtc
R6952 T10712 T10706 nummod 3,aatttcacttgttcagtagtc
R6953 T10713 T10706 punct ',aatttcacttgttcagtagtc
R6954 T10714 T10706 punct ),aatttcacttgttcagtagtc
R6955 T10715 T10660 punct ", ",Exon
R6956 T10716 T10660 appos Exon,Exon
R6957 T10717 T10716 nummod 5,Exon
R6958 T10718 T10716 punct : ,Exon
R6959 T10719 T10720 dep 128R,cctgcttctgaggatgaaaacgc
R6960 T10720 T10716 parataxis cctgcttctgaggatgaaaacgc,Exon
R6961 T10721 T10720 punct (,cctgcttctgaggatgaaaacgc
R6962 T10722 T10720 nummod 5,cctgcttctgaggatgaaaacgc
R6963 T10723 T10722 punct ',5
R6964 T10724 T10720 punct -,cctgcttctgaggatgaaaacgc
R6965 T10725 T10720 punct -,cctgcttctgaggatgaaaacgc
R6966 T10726 T10720 nummod 3,cctgcttctgaggatgaaaacgc
R6967 T10727 T10720 punct ',cctgcttctgaggatgaaaacgc
R6968 T10728 T10720 punct ),cctgcttctgaggatgaaaacgc
R6969 T10729 T10657 punct .,were
R6970 T10731 T10732 compound Rabbit,brain
R6971 T10732 T10733 compound brain,mRNA
R6972 T10733 T10736 nsubjpass mRNA,obtained
R6973 T10734 T10733 compound poly,mRNA
R6974 T10735 T10733 compound A+,mRNA
R6975 T10737 T10733 punct ", ",mRNA
R6976 T10738 T10739 compound mouse,testis
R6977 T10739 T10733 conj testis,mRNA
R6978 T10740 T10739 cc and,testis
R6979 T10741 T10742 amod human,brain
R6980 T10742 T10743 nmod brain,RNA
R6981 T10743 T10739 conj RNA,testis
R6982 T10744 T10743 amod total,RNA
R6983 T10745 T10736 auxpass were,obtained
R6984 T10746 T10736 prep from,obtained
R6985 T10747 T10748 compound BD,Clontech
R6986 T10748 T10746 pobj Clontech,from
R6987 T10749 T10748 compound Bioscience,Clontech
R6988 T10750 T10748 punct (,Clontech
R6989 T10751 T10752 compound Palo,Alto
R6990 T10752 T10748 appos Alto,Clontech
R6991 T10753 T10752 punct ", ",Alto
R6992 T10754 T10752 npadvmod CA,Alto
R6993 T10755 T10752 punct ", ",Alto
R6994 T10756 T10752 npadvmod U.S.A.,Alto
R6995 T10757 T10748 punct ),Clontech
R6996 T10758 T10736 punct .,obtained
R6997 T10760 T10761 amod Reverse,transcription
R6998 T10761 T10762 nsubjpass transcription,performed
R6999 T10763 T10761 cc and,transcription
R7000 T10764 T10761 conj PCR,transcription
R7001 T10765 T10762 auxpass was,performed
R7002 T10766 T10767 mark as,described
R7003 T10767 T10762 advcl described,performed
R7004 T10768 T10767 advmod previously,described
R7005 T10769 T10762 punct ", ",performed
R7006 T10770 T10762 advcl using,performed
R7007 T10771 T10772 preconj either,μg
R7008 T10772 T10770 dobj μg,using
R7009 T10773 T10772 nummod 1,μg
R7010 T10774 T10772 prep of,μg
R7011 T10775 T10776 amod total,RNA
R7012 T10776 T10774 pobj RNA,of
R7013 T10777 T10772 cc or,μg
R7014 T10778 T10779 nummod 50,ng
R7015 T10779 T10772 conj ng,μg
R7016 T10780 T10779 prep of,ng
R7017 T10781 T10782 compound poly,A+
R7018 T10782 T10783 compound A+,mRNA
R7019 T10783 T10780 pobj mRNA,of
R7020 T10784 T10770 prep as,using
R7021 T10785 T10784 pobj template,as
R7022 T10786 T10785 prep for,template
R7023 T10787 T10788 amod first,strand
R7024 T10788 T10789 compound strand,cDNA
R7025 T10789 T10790 compound cDNA,synthesis
R7026 T10790 T10786 pobj synthesis,for
R7027 T10791 T10762 punct .,performed
R7028 T10793 T10794 compound PCR,products
R7029 T10794 T10795 nsubjpass products,cloned
R7030 T10796 T10795 auxpass were,cloned
R7031 T10797 T10795 prep into,cloned
R7032 T10798 T10797 pobj pCR2.1,into
R7033 T10799 T10800 punct (,Invitrogen
R7034 T10800 T10798 parataxis Invitrogen,pCR2.1
R7035 T10801 T10800 punct ", ",Invitrogen
R7036 T10802 T10800 npadvmod Carlsbad,Invitrogen
R7037 T10803 T10800 npadvmod CA,Invitrogen
R7038 T10804 T10800 punct ", ",Invitrogen
R7039 T10805 T10800 npadvmod U.S.A.,Invitrogen
R7040 T10806 T10800 punct ),Invitrogen
R7041 T10807 T10795 cc and,cloned
R7042 T10808 T10795 conj transformed,cloned
R7043 T10809 T10808 prep into,transformed
R7044 T10810 T10811 poss InvαF,competent
R7045 T10811 T10813 amod competent,cells
R7046 T10812 T10810 case ',InvαF
R7047 T10813 T10809 pobj cells,into
R7048 T10814 T10795 punct .,cloned
R7049 T10816 T10817 compound Plasmid,inserts
R7050 T10817 T10818 nsubjpass inserts,sequenced
R7051 T10819 T10818 auxpass were,sequenced
R7052 T10820 T10818 agent by,sequenced
R7053 T10821 T10822 det the,Facility
R7054 T10822 T10820 pobj Facility,by
R7055 T10823 T10824 compound Roswell,Park
R7056 T10824 T10825 compound Park,Institute
R7057 T10825 T10822 compound Institute,Facility
R7058 T10826 T10825 compound Cancer,Institute
R7059 T10827 T10828 compound Biopolymer,Core
R7060 T10828 T10822 compound Core,Facility
R7061 T10829 T10818 punct .,sequenced
R7062 T10983 T10982 prep of,Deposition
R7063 T10984 T10985 compound nucleotide,sequences
R7064 T10985 T10983 pobj sequences,of
R7065 T10987 T10988 nsubjpass Sequences,deposited
R7066 T10989 T10987 prep from,Sequences
R7067 T10990 T10991 det this,article
R7068 T10991 T10989 pobj article,from
R7069 T10992 T10988 aux have,deposited
R7070 T10993 T10988 auxpass been,deposited
R7071 T10994 T10988 prep in,deposited
R7072 T10995 T10996 det the,database
R7073 T10996 T10994 pobj database,in
R7074 T10997 T10996 compound GenBank,database
R7075 T10998 T10988 prep with,deposited
R7076 T10999 T11000 det the,numbers
R7077 T11000 T10998 pobj numbers,with
R7078 T11001 T11000 amod following,numbers
R7079 T11002 T11000 compound accession,numbers
R7080 T11003 T11000 punct : ,numbers
R7081 T11004 T11005 nmod Homo,sapiens
R7082 T11005 T11006 nmod sapiens,S
R7083 T11006 T11000 appos S,numbers
R7084 T11007 T11006 nmod TACC1,S
R7085 T11008 T11009 amod short,isoform
R7086 T11009 T11006 compound isoform,S
R7087 T11010 T11011 punct (,AY177411
R7088 T11011 T11006 parataxis AY177411,S
R7089 T11012 T11011 punct ),AY177411
R7090 T11013 T11006 punct ", ",S
R7091 T11014 T11015 nmod Mus,musculus
R7092 T11015 T11016 nmod musculus,S
R7093 T11016 T11006 appos S,S
R7094 T11017 T11016 nmod TACC1,S
R7095 T11018 T11019 amod short,isoform
R7096 T11019 T11016 compound isoform,S
R7097 T11020 T11021 punct (,AY177412
R7098 T11021 T11016 parataxis AY177412,S
R7099 T11022 T11021 punct ),AY177412
R7100 T11023 T11006 punct ", ",S
R7101 T11024 T11025 nmod Mus,musculus
R7102 T11025 T11026 nmod musculus,A
R7103 T11026 T11006 appos A,S
R7104 T11027 T11026 nmod TACC1,A
R7105 T11028 T11029 amod long,isoform
R7106 T11029 T11026 compound isoform,A
R7107 T11030 T11031 punct (,AY177413
R7108 T11031 T11026 parataxis AY177413,A
R7109 T11032 T11031 punct ),AY177413
R7110 T11033 T11006 punct ", ",S
R7111 T11034 T11035 compound Mus,musculus
R7112 T11035 T11036 compound musculus,TACC2s
R7113 T11036 T11006 appos TACC2s,S
R7114 T11037 T11038 punct (,AY177410
R7115 T11038 T11036 parataxis AY177410,TACC2s
R7116 T11039 T11038 punct ),AY177410
R7117 T11040 T11006 punct ", ",S
R7118 T11041 T11042 compound Oryctolagus,cuniculus
R7119 T11042 T11043 compound cuniculus,TACC3
R7120 T11043 T11006 appos TACC3,S
R7121 T11044 T11045 punct (,AY161270
R7122 T11045 T11043 parataxis AY161270,TACC3
R7123 T11046 T11045 punct ),AY161270
R7124 T11047 T11006 punct ", ",S
R7125 T11048 T11049 compound Danio,rerio
R7126 T11049 T11050 compound rerio,TACC3
R7127 T11050 T11006 appos TACC3,S
R7128 T11051 T11052 punct (,AY170618
R7129 T11052 T11050 parataxis AY170618,TACC3
R7130 T11053 T11052 punct ),AY170618
R7131 T11054 T10988 punct .,deposited
R7132 T11056 T11057 nsubj Annotations,are
R7133 T11058 T11056 acl submitted,Annotations
R7134 T11059 T11058 prep to,submitted
R7135 T11060 T11061 det the,database
R7136 T11061 T11059 pobj database,to
R7137 T11062 T11063 amod Third,party
R7138 T11063 T11061 compound party,database
R7139 T11064 T11061 compound annotation,database
R7140 T11065 T11061 prep at,database
R7141 T11066 T11065 pobj NCBI,at
R7142 T11067 T11068 mark as,follows
R7143 T11068 T11057 advcl follows,are
R7144 T11069 T11057 punct : ,are
R7145 T11070 T11071 nmod Rattus,norvegus
R7146 T11071 T11072 nmod norvegus,A
R7147 T11072 T11057 dep A,are
R7148 T11073 T11072 nmod TACC1,A
R7149 T11074 T11075 amod long,isoform
R7150 T11075 T11072 compound isoform,A
R7151 T11076 T11077 punct (,BK001653
R7152 T11077 T11072 parataxis BK001653,A
R7153 T11078 T11077 punct ),BK001653
R7154 T11079 T11072 punct ", ",A
R7155 T11080 T11081 compound Takifugu,rubripes
R7156 T11081 T11082 compound rubripes,TACC1A
R7157 T11082 T11072 appos TACC1A,A
R7158 T11083 T11084 punct (,BK000667
R7159 T11084 T11082 parataxis BK000667,TACC1A
R7160 T11085 T11084 compound BK000666,BK000667
R7161 T11086 T11084 punct /,BK000667
R7162 T11087 T11084 punct ),BK000667
R7163 T11088 T11072 punct ", ",A
R7164 T11089 T11090 compound Takifugu,rubripes
R7165 T11090 T11091 compound rubripes,TACC1B
R7166 T11091 T11072 appos TACC1B,A
R7167 T11092 T11093 punct (,BK000664
R7168 T11093 T11091 parataxis BK000664,TACC1B
R7169 T11094 T11093 punct ),BK000664
R7170 T11095 T11072 punct ", ",A
R7171 T11096 T11097 compound Mus,musculus
R7172 T11097 T11098 compound musculus,TACC2l
R7173 T11098 T11072 appos TACC2l,A
R7174 T11099 T11100 punct (,BK001495
R7175 T11100 T11098 parataxis BK001495,TACC2l
R7176 T11101 T11100 punct ),BK001495
R7177 T11102 T11072 punct ", ",A
R7178 T11103 T11104 compound Rattus,norvegus
R7179 T11104 T11105 compound norvegus,TACC2l
R7180 T11105 T11072 appos TACC2l,A
R7181 T11106 T11107 punct (,BK001658
R7182 T11107 T11105 parataxis BK001658,TACC2l
R7183 T11108 T11107 punct ),BK001658
R7184 T11109 T11072 punct ", ",A
R7185 T11110 T11111 compound Rattus,norvegus
R7186 T11111 T11112 compound norvegus,TACC2s
R7187 T11112 T11072 appos TACC2s,A
R7188 T11113 T11114 punct (,BK001657
R7189 T11114 T11112 parataxis BK001657,TACC2s
R7190 T11115 T11114 punct ),BK001657
R7191 T11116 T11072 punct ", ",A
R7192 T11117 T11118 compound Takifugu,rubripes
R7193 T11118 T11119 compound rubripes,TACC2l
R7194 T11119 T11072 appos TACC2l,A
R7195 T11120 T11121 punct (,BK000690
R7196 T11121 T11119 parataxis BK000690,TACC2l
R7197 T11122 T11121 punct ),BK000690
R7198 T11123 T11072 punct ", ",A
R7199 T11124 T11125 compound Rattus,norvegus
R7200 T11125 T11126 compound norvegus,TACC3
R7201 T11126 T11072 appos TACC3,A
R7202 T11127 T11128 punct (,BK001491
R7203 T11128 T11126 parataxis BK001491,TACC3
R7204 T11129 T11128 punct ),BK001491
R7205 T11130 T11072 punct ", ",A
R7206 T11131 T11132 compound Gallus,gallus
R7207 T11132 T11133 compound gallus,TACC3
R7208 T11133 T11072 appos TACC3,A
R7209 T11134 T11135 punct (,BK001482
R7210 T11135 T11133 parataxis BK001482,TACC3
R7211 T11136 T11135 punct ),BK001482
R7212 T11137 T11072 punct ", ",A
R7213 T11138 T11139 compound Silurana,tropicalis
R7214 T11139 T11140 compound tropicalis,TACC3
R7215 T11140 T11072 appos TACC3,A
R7216 T11141 T11142 punct (,BK001481
R7217 T11142 T11140 parataxis BK001481,TACC3
R7218 T11143 T11142 punct ),BK001481
R7219 T11144 T11072 punct ",",A
R7220 T11145 T11146 compound Takifugu,rubripes
R7221 T11146 T11147 compound rubripes,TACC3
R7222 T11147 T11072 appos TACC3,A
R7223 T11148 T11149 punct (,BK000649
R7224 T11149 T11147 parataxis BK000649,TACC3
R7225 T11150 T11149 punct ),BK000649
R7226 T11151 T11072 punct ", ",A
R7227 T11152 T11153 compound Takifugu,rubripes
R7228 T11153 T11154 compound rubripes,RHAMM
R7229 T11154 T11072 appos RHAMM,A
R7230 T11155 T11156 punct (,BK000676
R7231 T11156 T11154 parataxis BK000676,RHAMM
R7232 T11157 T11156 punct ),BK000676
R7233 T11158 T11072 punct ", ",A
R7234 T11159 T11160 compound Ciona,intestinalis
R7235 T11160 T11161 compound intestinalis,RHAMM
R7236 T11161 T11072 appos RHAMM,A
R7237 T11162 T11163 punct (,BK001479
R7238 T11163 T11161 parataxis BK001479,RHAMM
R7239 T11164 T11163 punct ),BK001479
R7240 T11165 T11072 punct ", ",A
R7241 T11166 T11167 compound Takifugu,rubripes
R7242 T11167 T11168 compound rubripes,Keratin
R7243 T11168 T11072 appos Keratin,A
R7244 T11169 T11170 punct (,BK000677
R7245 T11170 T11168 parataxis BK000677,Keratin
R7246 T11171 T11170 punct ),BK000677
R7247 T11172 T11072 punct ", ",A
R7248 T11173 T11174 compound Takifugu,rubripes
R7249 T11174 T11175 compound rubripes,TPM1
R7250 T11175 T11072 appos TPM1,A
R7251 T11176 T11177 punct (,BAC10576
R7252 T11177 T11175 parataxis BAC10576,TPM1
R7253 T11178 T11177 punct ),BAC10576
R7254 T11179 T11072 punct ", ",A
R7255 T11180 T11181 compound Ciona,intestinalis
R7256 T11181 T11182 compound intestinalis,Kif3b
R7257 T11182 T11072 appos Kif3b,A
R7258 T11183 T11184 punct (,BK001492
R7259 T11184 T11182 parataxis BK001492,Kif3b
R7260 T11185 T11184 punct ),BK001492
R7261 T11186 T11072 punct ", ",A
R7262 T11187 T11188 compound Ciona,intestinalis
R7263 T11188 T11189 compound intestinalis,klp2
R7264 T11189 T11072 appos klp2,A
R7265 T11190 T11191 punct (,BK001493
R7266 T11191 T11189 parataxis BK001493,klp2
R7267 T11192 T11191 punct ),BK001493
R7268 T11193 T11057 punct .,are
R7269 T11434 T11435 amod Phylogenetic,analysis
R7270 T11437 T11438 prep In,retrieved
R7271 T11439 T11437 pobj order,In
R7272 T11440 T11441 aux to,examine
R7273 T11441 T11439 acl examine,order
R7274 T11442 T11443 amod evolutionary,relationships
R7275 T11443 T11441 dobj relationships,examine
R7276 T11444 T11443 prep of,relationships
R7277 T11445 T11444 pobj proteins,of
R7278 T11446 T11445 acl containing,proteins
R7279 T11447 T11448 amod coiled,coil
R7280 T11448 T11449 compound coil,domains
R7281 T11449 T11446 dobj domains,containing
R7282 T11450 T11438 punct ", ",retrieved
R7283 T11451 T11452 compound protein,sequences
R7284 T11452 T11438 nsubjpass sequences,retrieved
R7285 T11453 T11452 acl representing,sequences
R7286 T11454 T11455 det the,members
R7287 T11455 T11453 dobj members,representing
R7288 T11456 T11455 amod major,members
R7289 T11457 T11455 prep of,members
R7290 T11458 T11459 det this,family
R7291 T11459 T11457 pobj family,of
R7292 T11460 T11459 amod super,family
R7293 T11461 T11452 punct ", ",sequences
R7294 T11462 T11452 prep including,sequences
R7295 T11463 T11462 pobj TACC,including
R7296 T11464 T11463 punct ", ",TACC
R7297 T11465 T11463 conj RHAMM,TACC
R7298 T11466 T11465 punct ", ",RHAMM
R7299 T11467 T11465 conj KLP,RHAMM
R7300 T11468 T11467 punct ", ",KLP
R7301 T11469 T11467 conj keratin,KLP
R7302 T11470 T11469 cc and,keratin
R7303 T11471 T11469 conj tropomyosin,keratin
R7304 T11472 T11452 prep from,sequences
R7305 T11473 T11474 amod available,vertebrates
R7306 T11474 T11472 pobj vertebrates,from
R7307 T11475 T11474 cc and,vertebrates
R7308 T11476 T11477 poss their,orthologues
R7309 T11477 T11474 conj orthologues,vertebrates
R7310 T11478 T11477 amod recognizable,orthologues
R7311 T11479 T11477 prep from,orthologues
R7312 T11480 T11481 det the,urochordate
R7313 T11481 T11479 pobj urochordate,from
R7314 T11482 T11483 compound Ciona,intestinalis
R7315 T11483 T11481 appos intestinalis,urochordate
R7316 T11484 T11481 punct ", ",urochordate
R7317 T11485 T11486 compound Drosophila,melanogaster
R7318 T11486 T11481 conj melanogaster,urochordate
R7319 T11487 T11486 punct ", ",melanogaster
R7320 T11488 T11489 compound C.,elegans
R7321 T11489 T11486 conj elegans,melanogaster
R7322 T11490 T11489 cc and,elegans
R7323 T11491 T11492 compound Saccharomyces,cerevisiae
R7324 T11492 T11489 conj cerevisiae,elegans
R7325 T11493 T11438 auxpass were,retrieved
R7326 T11494 T11438 preconj either,retrieved
R7327 T11495 T11438 advmod directly,retrieved
R7328 T11496 T11438 prep from,retrieved
R7329 T11497 T11498 compound NCBI,databases
R7330 T11498 T11496 pobj databases,from
R7331 T11499 T11498 compound sequence,databases
R7332 T11500 T11498 punct ", ",databases
R7333 T11501 T11502 advmod newly,predicted
R7334 T11502 T11498 acl predicted,databases
R7335 T11503 T11438 cc or,retrieved
R7336 T11504 T11438 conj isolated,retrieved
R7337 T11505 T11506 punct (,see
R7338 T11506 T11504 parataxis see,isolated
R7339 T11507 T11506 advmod above,see
R7340 T11508 T11506 punct ),see
R7341 T11509 T11438 punct .,retrieved
R7342 T11511 T11512 compound Species,abbreviations
R7343 T11512 T11513 nsubj abbreviations,are
R7344 T11514 T11515 mark as,follows
R7345 T11515 T11513 advcl follows,are
R7346 T11516 T11513 punct : ,are
R7347 T11517 T11513 dep hs,are
R7348 T11518 T11517 punct (,hs
R7349 T11519 T11520 compound Homo,sapiens
R7350 T11520 T11517 appos sapiens,hs
R7351 T11521 T11517 punct ),hs
R7352 T11522 T11517 punct ", ",hs
R7353 T11523 T11517 appos mm,hs
R7354 T11524 T11523 punct (,mm
R7355 T11525 T11526 compound Mus,musculus
R7356 T11526 T11523 appos musculus,mm
R7357 T11527 T11517 punct ),hs
R7358 T11528 T11517 punct ", ",hs
R7359 T11529 T11517 appos rn,hs
R7360 T11530 T11529 punct (,rn
R7361 T11531 T11532 compound Rattus,norvegus
R7362 T11532 T11529 appos norvegus,rn
R7363 T11533 T11517 punct ),hs
R7364 T11534 T11517 punct ", ",hs
R7365 T11535 T11517 appos oc,hs
R7366 T11536 T11535 punct (,oc
R7367 T11537 T11538 compound Oryctolagus,cuniculus
R7368 T11538 T11535 appos cuniculus,oc
R7369 T11539 T11517 punct ),hs
R7370 T11540 T11517 punct ", ",hs
R7371 T11541 T11517 appos gg,hs
R7372 T11542 T11541 punct (,gg
R7373 T11543 T11544 compound Gallus,gallus
R7374 T11544 T11541 appos gallus,gg
R7375 T11545 T11517 punct ),hs
R7376 T11546 T11517 punct ", ",hs
R7377 T11547 T11517 appos xl,hs
R7378 T11548 T11547 punct (,xl
R7379 T11549 T11550 compound Xenopus,laevis
R7380 T11550 T11547 appos laevis,xl
R7381 T11551 T11517 punct ),hs
R7382 T11552 T11517 punct ", ",hs
R7383 T11553 T11517 appos st,hs
R7384 T11554 T11553 punct (,st
R7385 T11555 T11556 compound Silurana,tropicalis
R7386 T11556 T11553 appos tropicalis,st
R7387 T11557 T11517 punct ),hs
R7388 T11558 T11517 punct ", ",hs
R7389 T11559 T11517 appos tr,hs
R7390 T11560 T11559 punct (,tr
R7391 T11561 T11562 compound Takifugu,rubripes
R7392 T11562 T11559 appos rubripes,tr
R7393 T11563 T11517 punct ),hs
R7394 T11564 T11517 punct ", ",hs
R7395 T11565 T11517 appos dr,hs
R7396 T11566 T11565 punct (,dr
R7397 T11567 T11568 compound Danio,rerio
R7398 T11568 T11565 appos rerio,dr
R7399 T11569 T11517 punct ),hs
R7400 T11570 T11517 punct ", ",hs
R7401 T11571 T11517 appos ci,hs
R7402 T11572 T11571 punct (,ci
R7403 T11573 T11574 compound Ciona,intestinalis
R7404 T11574 T11571 appos intestinalis,ci
R7405 T11575 T11517 punct ),hs
R7406 T11576 T11517 punct ", ",hs
R7407 T11577 T11517 appos dm,hs
R7408 T11578 T11577 punct (,dm
R7409 T11579 T11580 compound D.,melanogaster
R7410 T11580 T11577 appos melanogaster,dm
R7411 T11581 T11517 punct ),hs
R7412 T11582 T11517 punct ", ",hs
R7413 T11583 T11517 appos ce,hs
R7414 T11584 T11583 punct (,ce
R7415 T11585 T11586 compound C.,elegans
R7416 T11586 T11583 appos elegans,ce
R7417 T11587 T11517 punct ),hs
R7418 T11588 T11517 punct ", ",hs
R7419 T11589 T11517 appos sc,hs
R7420 T11590 T11589 punct (,sc
R7421 T11591 T11592 compound Saccharomyces,cerevisiae
R7422 T11592 T11589 appos cerevisiae,sc
R7423 T11593 T11517 punct ),hs
R7424 T11594 T11513 punct .,are
R7425 T11596 T11597 det The,sequences
R7426 T11597 T11598 nsubjpass sequences,used
R7427 T11599 T11597 acl identified,sequences
R7428 T11600 T11599 advmod above,identified
R7429 T11601 T11597 cc and,sequences
R7430 T11602 T11603 det the,translations
R7431 T11603 T11597 conj translations,sequences
R7432 T11604 T11603 amod following,translations
R7433 T11605 T11603 nmod protein,translations
R7434 T11606 T11605 cc or,protein
R7435 T11607 T11605 conj predicted,protein
R7436 T11608 T11598 auxpass were,used
R7437 T11609 T11598 prep for,used
R7438 T11610 T11611 amod phylogenetic,analysis
R7439 T11611 T11609 pobj analysis,for
R7440 T11612 T11598 punct : ,used
R7441 T11613 T11598 dep hsTACC1A,used
R7442 T11614 T11615 punct (,NP_006274
R7443 T11615 T11613 parataxis NP_006274,hsTACC1A
R7444 T11616 T11615 punct ),NP_006274
R7445 T11617 T11613 punct ", ",hsTACC1A
R7446 T11618 T11613 appos hsTACC2l,hsTACC1A
R7447 T11619 T11620 punct (,AAO62630
R7448 T11620 T11618 parataxis AAO62630,hsTACC2l
R7449 T11621 T11620 punct ),AAO62630
R7450 T11622 T11613 appos hsTACC2s,hsTACC1A
R7451 T11623 T11624 punct (,AAO62629
R7452 T11624 T11622 parataxis AAO62629,hsTACC2s
R7453 T11625 T11624 punct ),AAO62629
R7454 T11626 T11613 punct ", ",hsTACC1A
R7455 T11627 T11613 appos hsTACC3,hsTACC1A
R7456 T11628 T11629 punct (,NP_006333
R7457 T11629 T11627 parataxis NP_006333,hsTACC3
R7458 T11630 T11629 punct ),NP_006333
R7459 T11631 T11613 punct ", ",hsTACC1A
R7460 T11632 T11613 appos mmTACC3,hsTACC1A
R7461 T11633 T11634 punct (,Q9JJ11
R7462 T11634 T11632 parataxis Q9JJ11,mmTACC3
R7463 T11635 T11634 punct ),Q9JJ11
R7464 T11636 T11613 punct ", ",hsTACC1A
R7465 T11637 T11613 appos xlMaskin,hsTACC1A
R7466 T11638 T11639 punct (,Q9PTG8
R7467 T11639 T11637 parataxis Q9PTG8,xlMaskin
R7468 T11640 T11639 punct ),Q9PTG8
R7469 T11641 T11613 punct ", ",hsTACC1A
R7470 T11642 T11613 appos dmTACC,hsTACC1A
R7471 T11643 T11644 punct (,AAF52099
R7472 T11644 T11642 parataxis AAF52099,dmTACC
R7473 T11645 T11644 punct ),AAF52099
R7474 T11646 T11613 punct ", ",hsTACC1A
R7475 T11647 T11613 appos ceTAC1,hsTACC1A
R7476 T11648 T11649 punct (,NP_497059
R7477 T11649 T11647 parataxis NP_497059,ceTAC1
R7478 T11650 T11649 punct ),NP_497059
R7479 T11651 T11613 punct ", ",hsTACC1A
R7480 T11652 T11613 appos scSPC72,hsTACC1A
R7481 T11653 T11654 punct (,NP_009352
R7482 T11654 T11652 parataxis NP_009352,scSPC72
R7483 T11655 T11654 punct ),NP_009352
R7484 T11656 T11613 punct ", ",hsTACC1A
R7485 T11657 T11613 appos hsRHAMM,hsTACC1A
R7486 T11658 T11659 punct (,NP_036616
R7487 T11659 T11657 parataxis NP_036616,hsRHAMM
R7488 T11660 T11659 punct ),NP_036616
R7489 T11661 T11613 punct ", ",hsTACC1A
R7490 T11662 T11613 appos mmRHAMM,hsTACC1A
R7491 T11663 T11664 punct (,NP_038580
R7492 T11664 T11662 parataxis NP_038580,mmRHAMM
R7493 T11665 T11664 punct ),NP_038580
R7494 T11666 T11613 punct ", ",hsTACC1A
R7495 T11667 T11613 appos rnRHAMM,hsTACC1A
R7496 T11668 T11669 punct (,NP_037096
R7497 T11669 T11667 parataxis NP_037096,rnRHAMM
R7498 T11670 T11669 punct ),NP_037096
R7499 T11671 T11613 punct ", ",hsTACC1A
R7500 T11672 T11613 appos drRHAMM,hsTACC1A
R7501 T11673 T11674 punct (,AAQ97980
R7502 T11674 T11672 parataxis AAQ97980,drRHAMM
R7503 T11675 T11674 punct ),AAQ97980
R7504 T11676 T11613 punct ", ",hsTACC1A
R7505 T11677 T11613 appos hsKeratin,hsTACC1A
R7506 T11678 T11679 punct (,CAB76828
R7507 T11679 T11677 parataxis CAB76828,hsKeratin
R7508 T11680 T11679 punct ),CAB76828
R7509 T11681 T11613 punct ", ",hsTACC1A
R7510 T11682 T11613 appos mmKeratin,hsTACC1A
R7511 T11683 T11684 punct (,A61368
R7512 T11684 T11682 parataxis A61368,mmKeratin
R7513 T11685 T11684 punct ),A61368
R7514 T11686 T11613 punct ", ",hsTACC1A
R7515 T11687 T11613 appos rnKeratin,hsTACC1A
R7516 T11688 T11689 punct (,XP_235679
R7517 T11689 T11687 parataxis XP_235679,rnKeratin
R7518 T11690 T11689 punct ),XP_235679
R7519 T11691 T11613 punct ", ",hsTACC1A
R7520 T11692 T11613 appos hsTPM1,hsTACC1A
R7521 T11693 T11694 punct (,NP_000357
R7522 T11694 T11692 parataxis NP_000357,hsTPM1
R7523 T11695 T11694 punct ),NP_000357
R7524 T11696 T11613 punct ", ",hsTACC1A
R7525 T11697 T11613 appos mmTPM1,hsTACC1A
R7526 T11698 T11699 punct (,NP_077745
R7527 T11699 T11697 parataxis NP_077745,mmTPM1
R7528 T11700 T11699 punct ),NP_077745
R7529 T11701 T11613 punct ", ",hsTACC1A
R7530 T11702 T11613 appos rnTPM1,hsTACC1A
R7531 T11703 T11704 punct (,NP_62004
R7532 T11704 T11702 parataxis NP_62004,rnTPM1
R7533 T11705 T11613 punct ", ",hsTACC1A
R7534 T11706 T11613 appos drTPM1,hsTACC1A
R7535 T11707 T11708 punct (,NP_571180
R7536 T11708 T11706 parataxis NP_571180,drTPM1
R7537 T11709 T11708 punct ),NP_571180
R7538 T11710 T11613 appos dmTPM1,hsTACC1A
R7539 T11711 T11712 punct (,P06754
R7540 T11712 T11710 parataxis P06754,dmTPM1
R7541 T11713 T11712 punct ),P06754
R7542 T11714 T11613 punct ", ",hsTACC1A
R7543 T11715 T11613 appos ceTPM,hsTACC1A
R7544 T11716 T11717 punct (,NP_493540
R7545 T11717 T11715 parataxis NP_493540,ceTPM
R7546 T11718 T11717 punct ),NP_493540
R7547 T11719 T11613 appos scTPM1,hsTACC1A
R7548 T11720 T11721 punct (,P17536
R7549 T11721 T11719 parataxis P17536,scTPM1
R7550 T11722 T11721 punct ),P17536
R7551 T11723 T11613 punct ", ",hsTACC1A
R7552 T11724 T11613 appos hsKLP2,hsTACC1A
R7553 T11725 T11726 punct (,BAB03309
R7554 T11726 T11724 parataxis BAB03309,hsKLP2
R7555 T11727 T11726 punct ),BAB03309
R7556 T11728 T11613 punct ", ",hsTACC1A
R7557 T11729 T11613 appos rnKIF15,hsTACC1A
R7558 T11730 T11731 punct (,AAP44513
R7559 T11731 T11729 parataxis AAP44513,rnKIF15
R7560 T11732 T11731 punct ),AAP44513
R7561 T11733 T11613 punct ", ",hsTACC1A
R7562 T11734 T11613 appos xlKLP2,hsTACC1A
R7563 T11735 T11736 punct (,CAA08879
R7564 T11736 T11734 parataxis CAA08879,xlKLP2
R7565 T11737 T11736 punct ),CAA08879
R7566 T11738 T11613 punct ", ",hsTACC1A
R7567 T11739 T11613 appos dmKLP2,hsTACC1A
R7568 T11740 T11741 punct (,NP_476818
R7569 T11741 T11739 parataxis NP_476818,dmKLP2
R7570 T11742 T11741 punct ),NP_476818
R7571 T11743 T11613 punct ", ",hsTACC1A
R7572 T11744 T11613 appos ceKLP18,hsTACC1A
R7573 T11745 T11746 punct (,AA034669
R7574 T11746 T11744 parataxis AA034669,ceKLP18
R7575 T11747 T11746 punct ),AA034669
R7576 T11748 T11613 punct ", ",hsTACC1A
R7577 T11749 T11613 appos hsKIF3A,hsTACC1A
R7578 T11750 T11751 punct (,Q9Y496
R7579 T11751 T11749 parataxis Q9Y496,hsKIF3A
R7580 T11752 T11751 punct ),Q9Y496
R7581 T11753 T11613 punct ", ",hsTACC1A
R7582 T11754 T11613 appos mmKIF3A,hsTACC1A
R7583 T11755 T11756 punct (,NP_032469
R7584 T11756 T11754 parataxis NP_032469,mmKIF3A
R7585 T11757 T11756 punct ),NP_032469
R7586 T11758 T11613 punct ", ",hsTACC1A
R7587 T11759 T11613 appos rnKIF3A,hsTACC1A
R7588 T11760 T11761 punct (,XP_340797
R7589 T11761 T11759 parataxis XP_340797,rnKIF3A
R7590 T11762 T11761 punct ),XP_340797
R7591 T11763 T11613 punct ", ",hsTACC1A
R7592 T11764 T11613 appos xlKIF3A,hsTACC1A
R7593 T11765 T11766 punct (,CAA08879
R7594 T11766 T11764 parataxis CAA08879,xlKIF3A
R7595 T11767 T11766 punct ),CAA08879
R7596 T11768 T11613 punct ", ",hsTACC1A
R7597 T11769 T11613 appos ceKLP11,hsTACC1A
R7598 T11770 T11771 punct (,NP_741473
R7599 T11771 T11769 parataxis NP_741473,ceKLP11
R7600 T11772 T11771 punct ),NP_741473
R7601 T11773 T11613 punct ", ",hsTACC1A
R7602 T11774 T11613 appos ciKIF3,hsTACC1A
R7603 T11775 T11776 punct (,ci0100148992
R7604 T11776 T11774 parataxis ci0100148992,ciKIF3
R7605 T11777 T11776 punct ),ci0100148992
R7606 T11778 T11613 punct ", ",hsTACC1A
R7607 T11779 T11613 appos hsKIF3B,hsTACC1A
R7608 T11780 T11781 punct (,NP_004789
R7609 T11781 T11779 parataxis NP_004789,hsKIF3B
R7610 T11782 T11781 punct ),NP_004789
R7611 T11783 T11613 punct ", ",hsTACC1A
R7612 T11784 T11613 appos mmKIF3B,hsTACC1A
R7613 T11785 T11786 punct (,NP_004789
R7614 T11786 T11784 parataxis NP_004789,mmKIF3B
R7615 T11787 T11786 punct ),NP_004789
R7616 T11788 T11613 punct ", ",hsTACC1A
R7617 T11789 T11613 appos rnKIF3B,hsTACC1A
R7618 T11790 T11791 punct (,XP_215883
R7619 T11791 T11789 parataxis XP_215883,rnKIF3B
R7620 T11792 T11791 punct ),XP_215883
R7621 T11793 T11613 punct ", ",hsTACC1A
R7622 T11794 T11613 appos dmKIF3B,hsTACC1A
R7623 T11795 T11796 punct (,NP_524029
R7624 T11796 T11794 parataxis NP_524029,dmKIF3B
R7625 T11797 T11796 punct ),NP_524029
R7626 T11798 T11613 punct ", ",hsTACC1A
R7627 T11799 T11613 appos hsKIF3C,hsTACC1A
R7628 T11800 T11801 punct (,NP_002245
R7629 T11801 T11799 parataxis NP_002245,hsKIF3C
R7630 T11802 T11801 punct ),NP_002245
R7631 T11803 T11613 punct ", ",hsTACC1A
R7632 T11804 T11613 appos mmKIF3C,hsTACC1A
R7633 T11805 T11806 punct (,NP_032471
R7634 T11806 T11804 parataxis NP_032471,mmKIF3C
R7635 T11807 T11806 punct ),NP_032471
R7636 T11808 T11613 punct ", ",hsTACC1A
R7637 T11809 T11613 appos rnKIF3C,hsTACC1A
R7638 T11810 T11811 punct (,NP_445938
R7639 T11811 T11809 parataxis NP_445938,rnKIF3C
R7640 T11812 T11811 punct ),NP_445938
R7641 T11813 T11613 punct ", ",hsTACC1A
R7642 T11814 T11613 appos dmKIF3C,hsTACC1A
R7643 T11815 T11816 punct (,NP_651939
R7644 T11816 T11814 parataxis NP_651939,dmKIF3C
R7645 T11817 T11816 punct ),NP_651939
R7646 T11818 T11598 punct .,used
R7647 T11820 T11821 det These,sequences
R7648 T11821 T11823 nsubjpass sequences,aligned
R7649 T11822 T11821 compound protein,sequences
R7650 T11824 T11823 auxpass were,aligned
R7651 T11825 T11823 advmod initially,aligned
R7652 T11826 T11823 prep with,aligned
R7653 T11827 T11828 compound CLUSTAL,X
R7654 T11828 T11826 pobj X,with
R7655 T11829 T11830 punct [,38
R7656 T11830 T11823 parataxis 38,aligned
R7657 T11831 T11830 punct ],38
R7658 T11832 T11823 punct .,aligned
R7659 T11834 T11835 amod Minor,adjustments
R7660 T11835 T11836 nsubjpass adjustments,made
R7661 T11836 T11847 ccomp made,saved
R7662 T11837 T11835 prep to,adjustments
R7663 T11838 T11839 amod certain,regions
R7664 T11839 T11837 pobj regions,to
R7665 T11840 T11839 prep of,regions
R7666 T11841 T11842 det the,alignment
R7667 T11842 T11840 pobj alignment,of
R7668 T11843 T11835 prep for,adjustments
R7669 T11844 T11845 compound optimization,purposes
R7670 T11845 T11843 pobj purposes,for
R7671 T11846 T11836 auxpass were,made
R7672 T11848 T11836 prep based,made
R7673 T11849 T11848 prep on,based
R7674 T11850 T11851 amod pairwise,alignments
R7675 T11851 T11849 pobj alignments,on
R7676 T11852 T11847 punct ", ",saved
R7677 T11853 T11854 det the,output
R7678 T11854 T11847 nsubj output,saved
R7679 T11855 T11847 prep in,saved
R7680 T11856 T11857 compound PHYLIP,format
R7681 T11857 T11855 pobj format,in
R7682 T11858 T11847 punct ", ",saved
R7683 T11859 T11860 prep after,calculated
R7684 T11860 T11847 advcl calculated,saved
R7685 T11861 T11859 pobj which,after
R7686 T11862 T11860 punct ", ",calculated
R7687 T11863 T11864 det the,distances
R7688 T11864 T11860 nsubjpass distances,calculated
R7689 T11865 T11864 prep between,distances
R7690 T11866 T11865 pobj proteins,between
R7691 T11867 T11860 auxpass were,calculated
R7692 T11868 T11860 advcl using,calculated
R7693 T11869 T11870 compound Poisson,correction
R7694 T11870 T11868 dobj correction,using
R7695 T11871 T11860 cc and,calculated
R7696 T11872 T11873 det the,trees
R7697 T11873 T11875 nsubjpass trees,inferred
R7698 T11874 T11873 amod Unrooted,trees
R7699 T11875 T11860 conj inferred,calculated
R7700 T11876 T11875 auxpass were,inferred
R7701 T11877 T11875 prep with,inferred
R7702 T11878 T11879 det the,method
R7703 T11879 T11877 pobj method,with
R7704 T11880 T11879 compound NJ,method
R7705 T11881 T11875 cc and,inferred
R7706 T11882 T11883 advmod then,displayed
R7707 T11883 T11875 conj displayed,inferred
R7708 T11884 T11883 advcl using,displayed
R7709 T11885 T11884 dobj TreeView,using
R7710 T11886 T11887 punct [,39
R7711 T11887 T11883 parataxis 39,displayed
R7712 T11888 T11887 punct ],39
R7713 T11889 T11847 punct .,saved
R7714 T11891 T11892 compound Bootstrap,values
R7715 T11892 T11893 nsubjpass values,shown
R7716 T11894 T11892 prep above,values
R7717 T11895 T11894 pobj 700,above
R7718 T11896 T11892 prep for,values
R7719 T11897 T11898 nummod 1000,trials
R7720 T11898 T11896 pobj trials,for
R7721 T11899 T11893 auxpass are,shown
R7722 T11900 T11893 prep at,shown
R7723 T11901 T11902 det the,node
R7724 T11902 T11900 pobj node,at
R7725 T11903 T11893 punct .,shown
R7726 T11905 T11906 aux To,validate
R7727 T11906 T11907 advcl validate,analyzed
R7728 T11908 T11909 det the,tree
R7729 T11909 T11906 dobj tree,validate
R7730 T11910 T11907 punct ", ",analyzed
R7731 T11911 T11912 det the,set
R7732 T11912 T11907 nsubjpass set,analyzed
R7733 T11913 T11912 amod same,set
R7734 T11914 T11912 compound sequence,set
R7735 T11915 T11907 auxpass was,analyzed
R7736 T11916 T11907 prep with,analyzed
R7737 T11917 T11916 pobj tools,with
R7738 T11918 T11917 prep in,tools
R7739 T11919 T11920 det the,package
R7740 T11920 T11918 pobj package,in
R7741 T11921 T11920 compound PHYLIP,package
R7742 T11922 T11923 punct [,40
R7743 T11923 T11907 parataxis 40,analyzed
R7744 T11924 T11923 punct ],40
R7745 T11925 T11907 punct ", ",analyzed
R7746 T11926 T11907 advcl using,analyzed
R7747 T11927 T11926 dobj PRODIST,using
R7748 T11928 T11927 acl followed,PRODIST
R7749 T11929 T11928 agent by,followed
R7750 T11930 T11929 pobj FITCH,by
R7751 T11931 T11930 cc or,FITCH
R7752 T11932 T11930 conj NEIGNBOR,FITCH
R7753 T11933 T11926 cc and,using
R7754 T11934 T11935 dep tree,displaying
R7755 T11935 T11926 conj displaying,using
R7756 T11936 T11935 advcl using,displaying
R7757 T11937 T11936 dobj TreeView,using
R7758 T11938 T11907 punct .,analyzed
R7759 T11940 T11941 det This,method
R7760 T11941 T11943 nsubj method,produced
R7761 T11942 T11941 amod additional,method
R7762 T11944 T11943 dobj trees,produced
R7763 T11945 T11944 prep with,trees
R7764 T11946 T11947 advmod essentially,topology
R7765 T11947 T11945 pobj topology,with
R7766 T11948 T11947 det the,topology
R7767 T11949 T11947 amod same,topology
R7768 T11950 T11951 punct (,shown
R7769 T11951 T11943 parataxis shown,produced
R7770 T11952 T11951 nsubj data,shown
R7771 T11953 T11951 neg not,shown
R7772 T11954 T11951 punct ),shown
R7773 T11955 T11943 punct .,produced
R7774 T12120 T12121 advmod In,vitro
R7775 T12121 T12122 amod vitro,interaction
R7776 T12123 T12122 prep of,interaction
R7777 T12124 T12123 pobj TACC2,of
R7778 T12125 T12124 cc and,TACC2
R7779 T12126 T12124 conj RXRβ3,TACC2
R7780 T12128 T12129 det The,cDNA
R7781 T12129 T12131 nsubjpass cDNA,cloned
R7782 T12130 T12129 compound TACC2,cDNA
R7783 T12132 T12131 auxpass was,cloned
R7784 T12133 T12131 prep into,cloned
R7785 T12134 T12135 compound GST,pGEX5X2
R7786 T12135 T12133 pobj pGEX5X2,into
R7787 T12136 T12135 compound fusion,pGEX5X2
R7788 T12137 T12135 compound vector,pGEX5X2
R7789 T12138 T12139 punct (,Biosciences
R7790 T12139 T12131 parataxis Biosciences,cloned
R7791 T12140 T12139 compound Amersham,Biosciences
R7792 T12141 T12139 punct ", ",Biosciences
R7793 T12142 T12139 npadvmod Piscataway,Biosciences
R7794 T12143 T12139 punct ", ",Biosciences
R7795 T12144 T12139 npadvmod NJ,Biosciences
R7796 T12145 T12139 punct ", ",Biosciences
R7797 T12146 T12139 npadvmod USA,Biosciences
R7798 T12147 T12139 punct ),Biosciences
R7799 T12148 T12131 punct .,cloned
R7800 T12150 T12151 nmod GST,proteins
R7801 T12151 T12156 nsubjpass proteins,expressed
R7802 T12152 T12150 cc and,GST
R7803 T12153 T12154 compound GST,TACC2
R7804 T12154 T12150 conj TACC2,GST
R7805 T12155 T12154 punct -,TACC2
R7806 T12157 T12156 auxpass were,expressed
R7807 T12158 T12156 prep in,expressed
R7808 T12159 T12160 nmod E.,coli
R7809 T12160 T12161 nmod coli,S
R7810 T12161 T12158 pobj S,in
R7811 T12162 T12161 nmod BL21,S
R7812 T12163 T12164 punct (,DE3
R7813 T12164 T12161 parataxis DE3,S
R7814 T12165 T12164 punct ),DE3
R7815 T12166 T12161 nmod plys,S
R7816 T12167 T12161 punct """",S
R7817 T12168 T12161 punct """",S
R7818 T12169 T12156 prep with,expressed
R7819 T12170 T12171 nummod 1,mM
R7820 T12171 T12172 compound mM,IPTG
R7821 T12172 T12169 pobj IPTG,with
R7822 T12173 T12172 prep at,IPTG
R7823 T12174 T12175 nummod 37,°C
R7824 T12175 T12176 compound °C,shaker
R7825 T12176 T12173 pobj shaker,at
R7826 T12177 T12172 prep for,IPTG
R7827 T12178 T12179 nummod 2,hrs
R7828 T12179 T12177 pobj hrs,for
R7829 T12180 T12156 punct .,expressed
R7830 T12182 T12183 nsubjpass Cells,harvested
R7831 T12184 T12185 punct (,ml
R7832 T12185 T12182 parataxis ml,Cells
R7833 T12186 T12185 nummod 50,ml
R7834 T12187 T12185 punct ),ml
R7835 T12188 T12183 auxpass were,harvested
R7836 T12189 T12183 cc and,harvested
R7837 T12190 T12183 conj resuspended,harvested
R7838 T12191 T12190 prep in,resuspended
R7839 T12192 T12193 nummod 5,ml
R7840 T12193 T12191 pobj ml,in
R7841 T12194 T12193 prep of,ml
R7842 T12195 T12196 nummod 20,mM
R7843 T12196 T12197 compound mM,HCl
R7844 T12197 T12194 pobj HCl,of
R7845 T12198 T12197 compound Tris,HCl
R7846 T12199 T12197 punct -,HCl
R7847 T12200 T12197 npadvmod pH.8.0,HCl
R7848 T12201 T12197 punct ", ",HCl
R7849 T12202 T12203 nummod 200,mM
R7850 T12203 T12204 compound mM,NaCl
R7851 T12204 T12197 appos NaCl,HCl
R7852 T12205 T12197 punct ", ",HCl
R7853 T12206 T12207 nummod 1,mM
R7854 T12207 T12208 compound mM,EDTA
R7855 T12208 T12197 appos EDTA,HCl
R7856 T12209 T12208 npadvmod pH8.0,EDTA
R7857 T12210 T12197 punct ", ",HCl
R7858 T12211 T12212 compound Protease,inhibitor
R7859 T12212 T12213 compound inhibitor,set
R7860 T12213 T12197 appos set,HCl
R7861 T12214 T12213 nummod III,set
R7862 T12215 T12216 punct (,Calbiochem
R7863 T12216 T12213 parataxis Calbiochem,set
R7864 T12217 T12216 punct ),Calbiochem
R7865 T12218 T12183 punct .,harvested
R7866 T12220 T12221 det The,cells
R7867 T12221 T12222 nsubjpass cells,lysed
R7868 T12223 T12222 auxpass were,lysed
R7869 T12224 T12222 prep by,lysed
R7870 T12225 T12224 pobj sonication,by
R7871 T12226 T12222 cc and,lysed
R7872 T12227 T12228 nsubj lysate,cleared
R7873 T12228 T12222 conj cleared,lysed
R7874 T12229 T12228 prep by,cleared
R7875 T12230 T12229 pobj centrifugation,by
R7876 T12231 T12230 prep at,centrifugation
R7877 T12232 T12233 nummod 7500,rpm
R7878 T12233 T12231 pobj rpm,at
R7879 T12234 T12230 prep at,centrifugation
R7880 T12235 T12236 nummod 4,°C
R7881 T12236 T12234 pobj °C,at
R7882 T12237 T12230 prep for,centrifugation
R7883 T12238 T12239 nummod 15,min
R7884 T12239 T12237 pobj min,for
R7885 T12240 T12228 punct .,cleared
R7886 T12242 T12243 det The,lysate
R7887 T12243 T12245 nsubjpass lysate,immobilized
R7888 T12244 T12243 amod cleared,lysate
R7889 T12246 T12245 auxpass was,immobilized
R7890 T12247 T12245 prep on,immobilized
R7891 T12248 T12249 compound glutathione,sepharose
R7892 T12249 T12250 compound sepharose,beads
R7893 T12250 T12247 pobj beads,on
R7894 T12251 T12245 prep in,immobilized
R7895 T12252 T12253 nummod 3,ml
R7896 T12253 T12251 pobj ml,in
R7897 T12254 T12253 prep of,ml
R7898 T12255 T12256 nummod 20,mM
R7899 T12256 T12257 compound mM,HCl
R7900 T12257 T12254 pobj HCl,of
R7901 T12258 T12257 compound Tris,HCl
R7902 T12259 T12257 punct -,HCl
R7903 T12260 T12257 npadvmod pH.8.0,HCl
R7904 T12261 T12257 punct ", ",HCl
R7905 T12262 T12263 nummod 200,mM
R7906 T12263 T12264 compound mM,NaCl
R7907 T12264 T12257 appos NaCl,HCl
R7908 T12265 T12257 punct ", ",HCl
R7909 T12266 T12267 nummod 1,mM
R7910 T12267 T12268 compound mM,EDTA
R7911 T12268 T12257 appos EDTA,HCl
R7912 T12269 T12268 npadvmod pH8.0,EDTA
R7913 T12270 T12245 punct ),immobilized
R7914 T12271 T12245 punct .,immobilized
R7915 T12273 T12274 compound RXRβ3,cDNA
R7916 T12274 T12275 nsubjpass cDNA,cloned
R7917 T12276 T12275 auxpass was,cloned
R7918 T12277 T12275 prep into,cloned
R7919 T12278 T12279 compound pET,28C
R7920 T12279 T12277 pobj 28C,into
R7921 T12280 T12279 punct (,28C
R7922 T12281 T12279 punct +,28C
R7923 T12282 T12279 punct ),28C
R7924 T12283 T12284 punct (,Invitrogen
R7925 T12284 T12279 parataxis Invitrogen,28C
R7926 T12285 T12284 punct ", ",Invitrogen
R7927 T12286 T12284 npadvmod Carlsbad,Invitrogen
R7928 T12287 T12284 punct ", ",Invitrogen
R7929 T12288 T12284 npadvmod CA,Invitrogen
R7930 T12289 T12284 punct ", ",Invitrogen
R7931 T12290 T12284 npadvmod USA,Invitrogen
R7932 T12291 T12284 punct ),Invitrogen
R7933 T12292 T12275 cc and,cloned
R7934 T12293 T12294 nsubj protein,synthesized
R7935 T12294 T12275 conj synthesized,cloned
R7936 T12295 T12294 prep by,synthesized
R7937 T12296 T12297 nmod TNT,kit
R7938 T12297 T12295 pobj kit,by
R7939 T12298 T12299 advmod quick,coupled
R7940 T12299 T12297 amod coupled,kit
R7941 T12300 T12301 compound transcription,translation
R7942 T12301 T12297 compound translation,kit
R7943 T12302 T12301 punct /,translation
R7944 T12303 T12297 compound system,kit
R7945 T12304 T12305 punct (,Promega
R7946 T12305 T12297 parataxis Promega,kit
R7947 T12306 T12305 punct ),Promega
R7948 T12307 T12294 cc and,synthesized
R7949 T12308 T12294 conj radiolabeled,synthesized
R7950 T12309 T12308 prep with,radiolabeled
R7951 T12310 T12311 compound 35S,methionine
R7952 T12311 T12309 pobj methionine,with
R7953 T12312 T12308 prep according,radiolabeled
R7954 T12313 T12312 prep to,according
R7955 T12314 T12315 poss manufacturer,instructions
R7956 T12315 T12313 pobj instructions,to
R7957 T12316 T12314 case 's,manufacturer
R7958 T12317 T12294 punct .,synthesized
R7959 T12319 T12320 nummod 100,μl
R7960 T12320 T12321 nsubjpass μl,incubated
R7961 T12322 T12320 prep of,μl
R7962 T12323 T12324 advmod in,vitro
R7963 T12324 T12325 advmod vitro,translated
R7964 T12325 T12326 amod translated,protein
R7965 T12326 T12322 pobj protein,of
R7966 T12327 T12326 compound RXRβ3,protein
R7967 T12328 T12320 prep in,μl
R7968 T12329 T12330 nummod 1,ml
R7969 T12330 T12328 pobj ml,in
R7970 T12331 T12330 prep of,ml
R7971 T12332 T12333 nummod 20,mM
R7972 T12333 T12334 compound mM,HCl
R7973 T12334 T12331 pobj HCl,of
R7974 T12335 T12334 compound Tris,HCl
R7975 T12336 T12334 punct -,HCl
R7976 T12337 T12334 npadvmod pH.8.0,HCl
R7977 T12338 T12334 punct ", ",HCl
R7978 T12339 T12340 nummod 200,mM
R7979 T12340 T12341 compound mM,NaCl
R7980 T12341 T12334 appos NaCl,HCl
R7981 T12342 T12334 punct ", ",HCl
R7982 T12343 T12344 nummod 1,mM
R7983 T12344 T12345 compound mM,EDTA
R7984 T12345 T12334 appos EDTA,HCl
R7985 T12346 T12345 npadvmod pH8.0,EDTA
R7986 T12347 T12321 auxpass was,incubated
R7987 T12348 T12321 prep at,incubated
R7988 T12349 T12350 nummod 4,°C
R7989 T12350 T12348 pobj °C,at
R7990 T12351 T12321 prep with,incubated
R7991 T12352 T12353 amod immobilized,TACC2
R7992 T12353 T12351 pobj TACC2,with
R7993 T12354 T12353 compound GST,TACC2
R7994 T12355 T12353 punct -,TACC2
R7995 T12356 T12353 cc or,TACC2
R7996 T12357 T12353 conj GST,TACC2
R7997 T12358 T12321 prep for,incubated
R7998 T12359 T12360 nummod 90,min
R7999 T12360 T12358 pobj min,for
R8000 T12361 T12321 punct .,incubated
R8001 T12363 T12364 amod Unbound,RXRβ3
R8002 T12364 T12365 nsubjpass RXRβ3,removed
R8003 T12366 T12365 auxpass was,removed
R8004 T12367 T12365 prep by,removed
R8005 T12368 T12367 pcomp washing,by
R8006 T12369 T12370 nummod three,times
R8007 T12370 T12368 npadvmod times,washing
R8008 T12371 T12368 prep with,washing
R8009 T12372 T12373 nummod 20,mM
R8010 T12373 T12374 compound mM,HCl
R8011 T12374 T12371 pobj HCl,with
R8012 T12375 T12374 compound Tris,HCl
R8013 T12376 T12374 punct -,HCl
R8014 T12377 T12374 npadvmod pH.8.0,HCl
R8015 T12378 T12374 punct ", ",HCl
R8016 T12379 T12380 nummod 200,mM
R8017 T12380 T12381 compound mM,NaCl
R8018 T12381 T12374 appos NaCl,HCl
R8019 T12382 T12374 punct ", ",HCl
R8020 T12383 T12384 nummod 1,mM
R8021 T12384 T12385 compound mM,EDTA
R8022 T12385 T12374 appos EDTA,HCl
R8023 T12386 T12385 npadvmod pH8.0,EDTA
R8024 T12387 T12365 punct .,removed
R8025 T12389 T12390 compound Bound,proteins
R8026 T12390 T12391 nsubjpass proteins,eluted
R8027 T12392 T12391 auxpass were,eluted
R8028 T12393 T12391 prep from,eluted
R8029 T12394 T12395 det the,beads
R8030 T12395 T12393 pobj beads,from
R8031 T12396 T12391 prep at,eluted
R8032 T12397 T12398 compound room,temperature
R8033 T12398 T12396 pobj temperature,at
R8034 T12399 T12391 prep for,eluted
R8035 T12400 T12401 nummod 10,min
R8036 T12401 T12399 pobj min,for
R8037 T12402 T12391 prep in,eluted
R8038 T12403 T12404 compound elution,buffer
R8039 T12404 T12402 pobj buffer,in
R8040 T12405 T12406 punct (,HCl
R8041 T12406 T12404 parataxis HCl,buffer
R8042 T12407 T12408 nummod 100,mM
R8043 T12408 T12406 compound mM,HCl
R8044 T12409 T12406 compound Tris,HCl
R8045 T12410 T12406 punct ", ",HCl
R8046 T12411 T12406 npadvmod pH8.0,HCl
R8047 T12412 T12406 punct ", ",HCl
R8048 T12413 T12414 nummod 20,mM
R8049 T12414 T12415 nmod mM,glutathione
R8050 T12415 T12406 appos glutathione,HCl
R8051 T12416 T12415 amod reduced,glutathione
R8052 T12417 T12406 punct ),HCl
R8053 T12418 T12391 punct .,eluted
R8054 T12420 T12421 det The,proteins
R8055 T12421 T12422 nsubjpass proteins,analyzed
R8056 T12423 T12422 auxpass were,analyzed
R8057 T12424 T12422 prep on,analyzed
R8058 T12425 T12426 nummod 12,%
R8059 T12426 T12427 compound %,gels
R8060 T12427 T12424 pobj gels,on
R8061 T12428 T12427 compound SDS,gels
R8062 T12429 T12427 compound polyacrylamide,gels
R8063 T12430 T12422 punct .,analyzed
R8064 T12432 T12433 nmod Coomassie,staining
R8065 T12433 T12435 nsubj staining,verified
R8066 T12434 T12433 amod blue,staining
R8067 T12436 T12437 amod equal,loading
R8068 T12437 T12435 dobj loading,verified
R8069 T12438 T12437 prep of,loading
R8070 T12439 T12440 compound GST,protein
R8071 T12440 T12438 pobj protein,of
R8072 T12441 T12440 compound fusion,protein
R8073 T12442 T12435 punct .,verified
R8074 T12444 T12445 amod Dried,gels
R8075 T12445 T12446 nsubjpass gels,autoradiographed
R8076 T12447 T12446 auxpass were,autoradiographed
R8077 T12448 T12446 punct .,autoradiographed