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PMC:394351 / 7301-9583 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T2220 0-9 NN denotes Targeting
T2221 10-16 NN denotes vector
T2222 17-29 NN denotes construction
T2223 30-33 CC denotes and
T2224 34-44 NN denotes generation
T2225 45-47 IN denotes of
T2226 48-56 NN denotes knockout
T2227 57-61 NNS denotes mice
T2228 61-316 sentence denotes A mouse genomic bacterial artificial chromosome (BAC) library (CitbCJ7, ES cell line/129Sv, Research Genetics, Inc., Huntsville, AL, USA) was screened by using primers designed from the sequences of mouse Abcg5 and Abcg8 cDNA as previously reported [21].
T2229 62-63 DT denotes A
T2231 64-69 NN denotes mouse
T2232 70-77 JJ denotes genomic
T2233 78-87 JJ denotes bacterial
T2235 88-98 JJ denotes artificial
T2234 99-109 NN denotes chromosome
T2236 110-111 -LRB- denotes (
T2237 111-114 NN denotes BAC
T2238 114-115 -RRB- denotes )
T2230 116-123 NN denotes library
T2240 124-125 -LRB- denotes (
T2242 125-132 NN denotes CitbCJ7
T2243 132-134 , denotes ,
T2244 134-136 NN denotes ES
T2245 137-141 NN denotes cell
T2247 142-146 NN denotes line
T2248 146-147 HYPH denotes /
T2246 147-152 NN denotes 129Sv
T2249 152-154 , denotes ,
T2250 154-162 NNP denotes Research
T2251 163-171 NNP denotes Genetics
T2252 171-173 , denotes ,
T2253 173-177 NNP denotes Inc.
T2254 177-179 , denotes ,
T2241 179-189 NNP denotes Huntsville
T2255 189-191 , denotes ,
T2256 191-193 NNP denotes AL
T2257 193-195 , denotes ,
T2258 195-198 NNP denotes USA
T2259 198-199 -RRB- denotes )
T2260 200-203 VBD denotes was
T2239 204-212 VBN denotes screened
T2261 213-215 IN denotes by
T2262 216-221 VBG denotes using
T2263 222-229 NNS denotes primers
T2264 230-238 VBN denotes designed
T2265 239-243 IN denotes from
T2266 244-247 DT denotes the
T2267 248-257 NNS denotes sequences
T2268 258-260 IN denotes of
T2269 261-266 NN denotes mouse
T2271 267-272 NN denotes Abcg5
T2272 273-276 CC denotes and
T2273 277-282 NN denotes Abcg8
T2270 283-287 NN denotes cDNA
T2274 288-290 IN denotes as
T2276 291-301 RB denotes previously
T2275 302-310 VBN denotes reported
T2277 311-312 -LRB- denotes [
T2278 312-314 CD denotes 21
T2279 314-315 -RRB- denotes ]
T2280 315-316 . denotes .
T2281 316-403 sentence denotes A positive BAC clone was used as a template to amplify genomic DNA fragments of Abcg8.
T2282 317-318 DT denotes A
T2284 319-327 JJ denotes positive
T2285 328-331 NN denotes BAC
T2283 332-337 NN denotes clone
T2287 338-341 VBD denotes was
T2286 342-346 VBN denotes used
T2288 347-349 IN denotes as
T2289 350-351 DT denotes a
T2290 352-360 NN denotes template
T2291 361-363 TO denotes to
T2292 364-371 VB denotes amplify
T2293 372-379 JJ denotes genomic
T2295 380-383 NN denotes DNA
T2294 384-393 NNS denotes fragments
T2296 394-396 IN denotes of
T2297 397-402 NN denotes Abcg8
T2298 402-403 . denotes .
T2299 403-555 sentence denotes Long-fragment polymerase chain reaction (PCR) was performed using Expanded Long Template PCR system kit (Roche Applied Science, Indianapolis, IA, USA).
T2300 404-408 JJ denotes Long
T2302 408-409 HYPH denotes -
T2301 409-417 NN denotes fragment
T2304 418-428 NN denotes polymerase
T2306 429-434 NN denotes chain
T2305 435-443 NN denotes reaction
T2307 444-445 -LRB- denotes (
T2308 445-448 NN denotes PCR
T2309 448-449 -RRB- denotes )
T2310 450-453 VBD denotes was
T2303 454-463 VBN denotes performed
T2311 464-469 VBG denotes using
T2312 470-478 NN denotes Expanded
T2314 479-483 JJ denotes Long
T2316 484-492 NN denotes Template
T2315 493-496 NN denotes PCR
T2313 497-503 NN denotes system
T2317 504-507 NN denotes kit
T2318 508-509 -LRB- denotes (
T2320 509-514 NNP denotes Roche
T2321 515-522 NNP denotes Applied
T2319 523-530 NNP denotes Science
T2322 530-532 , denotes ,
T2323 532-544 NNP denotes Indianapolis
T2324 544-546 , denotes ,
T2325 546-548 NNP denotes IA
T2326 548-550 , denotes ,
T2327 550-553 NNP denotes USA
T2328 553-554 -RRB- denotes )
T2329 554-555 . denotes .
T2330 555-726 sentence denotes An approximately 4.5 kb 'long-arm' genomic fragment containing partial exon 1 to partial exon 3 was inserted into the Pml I restriction-cloning site A of OSDUPDEL vector.
T2331 556-558 DT denotes An
T2333 559-572 RB denotes approximately
T2334 573-576 CD denotes 4.5
T2335 577-579 NN denotes kb
T2336 580-581 `` denotes '
T2337 581-585 JJ denotes long
T2339 585-586 HYPH denotes -
T2338 586-589 NN denotes arm
T2340 589-590 '' denotes '
T2341 591-598 JJ denotes genomic
T2332 599-607 NN denotes fragment
T2343 608-618 VBG denotes containing
T2344 619-626 JJ denotes partial
T2345 627-631 NN denotes exon
T2346 632-633 CD denotes 1
T2347 634-636 IN denotes to
T2348 637-644 JJ denotes partial
T2349 645-649 NN denotes exon
T2350 650-651 CD denotes 3
T2351 652-655 VBD denotes was
T2342 656-664 VBN denotes inserted
T2352 665-669 IN denotes into
T2353 670-673 DT denotes the
T2355 674-677 NN denotes Pml
T2356 678-679 CD denotes I
T2357 680-691 NN denotes restriction
T2359 691-692 HYPH denotes -
T2358 692-699 VBG denotes cloning
T2360 700-704 NN denotes site
T2354 705-706 NN denotes A
T2361 707-709 IN denotes of
T2362 710-718 NN denotes OSDUPDEL
T2363 719-725 NN denotes vector
T2364 725-726 . denotes .
T2365 726-856 sentence denotes The 'short-arm' genomic fragment, containing partial exon 4 to partial exon 6, was cloned into the Not I-Kpn I of cloning site B.
T2366 727-730 DT denotes The
T2368 731-732 `` denotes '
T2369 732-737 NN denotes short
T2371 737-738 HYPH denotes -
T2370 738-741 NN denotes arm
T2372 741-742 '' denotes '
T2373 743-750 JJ denotes genomic
T2367 751-759 NN denotes fragment
T2375 759-761 , denotes ,
T2376 761-771 VBG denotes containing
T2377 772-779 JJ denotes partial
T2378 780-784 NN denotes exon
T2379 785-786 CD denotes 4
T2380 787-789 IN denotes to
T2381 790-797 JJ denotes partial
T2382 798-802 NN denotes exon
T2383 803-804 CD denotes 6
T2384 804-806 , denotes ,
T2385 806-809 VBD denotes was
T2374 810-816 VBN denotes cloned
T2386 817-821 IN denotes into
T2387 822-825 DT denotes the
T2389 826-829 NN denotes Not
T2390 830-831 NN denotes I
T2391 831-832 HYPH denotes -
T2388 832-835 NN denotes Kpn
T2392 836-837 CD denotes I
T2393 838-840 IN denotes of
T2394 841-848 VBG denotes cloning
T2396 849-853 NN denotes site
T2395 854-855 NN denotes B
T2397 855-856 . denotes .
T2398 856-1062 sentence denotes Homologous targeting would result in complete intron 3, partial exon 3 and partial exon 4 replacement by the neomycin-resistance cassette, resulting in the disruption of the ABC Walker A motif (Figure 1a).
T2399 857-867 JJ denotes Homologous
T2400 868-877 NN denotes targeting
T2402 878-883 MD denotes would
T2401 884-890 VB denotes result
T2403 891-893 IN denotes in
T2404 894-902 JJ denotes complete
T2405 903-909 NN denotes intron
T2406 910-911 CD denotes 3
T2407 911-913 , denotes ,
T2408 913-920 JJ denotes partial
T2409 921-925 NN denotes exon
T2410 926-927 CD denotes 3
T2411 928-931 CC denotes and
T2412 932-939 JJ denotes partial
T2413 940-944 NN denotes exon
T2415 945-946 CD denotes 4
T2414 947-958 NN denotes replacement
T2416 959-961 IN denotes by
T2417 962-965 DT denotes the
T2419 966-974 NN denotes neomycin
T2420 974-975 HYPH denotes -
T2421 975-985 JJ denotes resistance
T2418 986-994 NN denotes cassette
T2422 994-996 , denotes ,
T2423 996-1005 VBG denotes resulting
T2424 1006-1008 IN denotes in
T2425 1009-1012 DT denotes the
T2426 1013-1023 NN denotes disruption
T2427 1024-1026 IN denotes of
T2428 1027-1030 DT denotes the
T2430 1031-1034 NN denotes ABC
T2432 1035-1041 NN denotes Walker
T2431 1042-1043 NN denotes A
T2429 1044-1049 NN denotes motif
T2433 1050-1051 -LRB- denotes (
T2435 1051-1057 NN denotes Figure
T2434 1058-1060 NN denotes 1a
T2436 1060-1061 -RRB- denotes )
T2437 1061-1062 . denotes .
T2438 1062-1209 sentence denotes ES cells (129/SvEvTac-cell line) were electroporated with the linearized targeting vector DNA and cultured on sub-confluent embryonic fibroblasts.
T2439 1063-1065 NN denotes ES
T2440 1066-1071 NNS denotes cells
T2442 1072-1073 -LRB- denotes (
T2444 1073-1076 CD denotes 129
T2446 1076-1077 HYPH denotes /
T2445 1077-1084 NN denotes SvEvTac
T2447 1084-1085 HYPH denotes -
T2448 1085-1089 NN denotes cell
T2443 1090-1094 NN denotes line
T2449 1094-1095 -RRB- denotes )
T2450 1096-1100 VBD denotes were
T2441 1101-1115 VBN denotes electroporated
T2451 1116-1120 IN denotes with
T2452 1121-1124 DT denotes the
T2454 1125-1135 VBN denotes linearized
T2456 1136-1145 NN denotes targeting
T2455 1146-1152 NN denotes vector
T2453 1153-1156 NN denotes DNA
T2457 1157-1160 CC denotes and
T2458 1161-1169 VBN denotes cultured
T2459 1170-1172 IN denotes on
T2460 1173-1186 JJ denotes sub-confluent
T2462 1187-1196 JJ denotes embryonic
T2461 1197-1208 NNS denotes fibroblasts
T2463 1208-1209 . denotes .
T2464 1209-1345 sentence denotes Transfected cell colonies were selected by culturing cells in medium containing 200 μg/mL G418 (Life Technologies, Rockville, MD, USA).
T2465 1210-1221 VBN denotes Transfected
T2467 1222-1226 NN denotes cell
T2466 1227-1235 NNS denotes colonies
T2469 1236-1240 VBD denotes were
T2468 1241-1249 VBN denotes selected
T2470 1250-1252 IN denotes by
T2471 1253-1262 VBG denotes culturing
T2472 1263-1268 NNS denotes cells
T2473 1269-1271 IN denotes in
T2474 1272-1278 NN denotes medium
T2475 1279-1289 VBG denotes containing
T2476 1290-1293 CD denotes 200
T2477 1294-1296 NN denotes μg
T2479 1296-1297 SYM denotes /
T2480 1297-1299 NN denotes mL
T2478 1300-1304 NN denotes G418
T2481 1305-1306 -LRB- denotes (
T2483 1306-1310 NNP denotes Life
T2482 1311-1323 NNP denotes Technologies
T2484 1323-1325 , denotes ,
T2485 1325-1334 NNP denotes Rockville
T2486 1334-1336 , denotes ,
T2487 1336-1338 NNP denotes MD
T2488 1338-1340 , denotes ,
T2489 1340-1343 NNP denotes USA
T2490 1343-1344 -RRB- denotes )
T2491 1344-1345 . denotes .
T2492 1345-1619 sentence denotes The ES cells were screened for homologous recombination by PCR with the forward primer, neoF (5'-GGGTCGTTTGTTCGGATCAA-3') from neo cassette, and reverse primer intron 6R (5'-ACCAGTTGGTCCTAGCTCGA-3'), which is located outside the targeting construct in mouse Abcg8 intron 6.
T2493 1346-1349 DT denotes The
T2495 1350-1352 NN denotes ES
T2494 1353-1358 NNS denotes cells
T2497 1359-1363 VBD denotes were
T2496 1364-1372 VBN denotes screened
T2498 1373-1376 IN denotes for
T2499 1377-1387 JJ denotes homologous
T2500 1388-1401 NN denotes recombination
T2501 1402-1404 IN denotes by
T2502 1405-1408 NN denotes PCR
T2503 1409-1413 IN denotes with
T2504 1414-1417 DT denotes the
T2506 1418-1425 JJ denotes forward
T2505 1426-1432 NN denotes primer
T2507 1432-1434 , denotes ,
T2508 1434-1438 NN denotes neoF
T2509 1439-1440 -LRB- denotes (
T2510 1440-1441 CD denotes 5
T2512 1441-1442 SYM denotes '
T2513 1442-1443 HYPH denotes -
T2511 1443-1463 NN denotes GGGTCGTTTGTTCGGATCAA
T2514 1463-1464 HYPH denotes -
T2515 1464-1465 CD denotes 3
T2516 1465-1466 SYM denotes '
T2517 1466-1467 -RRB- denotes )
T2518 1468-1472 IN denotes from
T2519 1473-1476 NN denotes neo
T2520 1477-1485 NN denotes cassette
T2521 1485-1487 , denotes ,
T2522 1487-1490 CC denotes and
T2523 1491-1498 JJ denotes reverse
T2524 1499-1505 NN denotes primer
T2526 1506-1512 NN denotes intron
T2525 1513-1515 NN denotes 6R
T2527 1516-1517 -LRB- denotes (
T2528 1517-1518 CD denotes 5
T2530 1518-1519 SYM denotes '
T2531 1519-1520 HYPH denotes -
T2529 1520-1540 NN denotes ACCAGTTGGTCCTAGCTCGA
T2532 1540-1541 HYPH denotes -
T2533 1541-1542 CD denotes 3
T2534 1542-1543 SYM denotes '
T2535 1543-1544 -RRB- denotes )
T2536 1544-1546 , denotes ,
T2537 1546-1551 WDT denotes which
T2539 1552-1554 VBZ denotes is
T2538 1555-1562 VBN denotes located
T2540 1563-1570 IN denotes outside
T2541 1571-1574 DT denotes the
T2543 1575-1584 VBG denotes targeting
T2542 1585-1594 NN denotes construct
T2544 1595-1597 IN denotes in
T2545 1598-1603 NN denotes mouse
T2547 1604-1609 NN denotes Abcg8
T2546 1610-1616 NN denotes intron
T2548 1617-1618 CD denotes 6
T2549 1618-1619 . denotes .
T2550 1619-1823 sentence denotes Positively targeted ES cell clones were confirmed by Southern blotting, using ES cell DNA digested by BamHI and a probe comprising of a 562 bp PCR fragment from partial intron 2 and exon 3 of Abcg8 gene.
T2551 1620-1630 RB denotes Positively
T2552 1631-1639 VBN denotes targeted
T2554 1640-1642 NN denotes ES
T2555 1643-1647 NN denotes cell
T2553 1648-1654 NNS denotes clones
T2557 1655-1659 VBD denotes were
T2556 1660-1669 VBN denotes confirmed
T2558 1670-1672 IN denotes by
T2559 1673-1681 NNP denotes Southern
T2560 1682-1690 NN denotes blotting
T2561 1690-1692 , denotes ,
T2562 1692-1697 VBG denotes using
T2563 1698-1700 NN denotes ES
T2564 1701-1705 NN denotes cell
T2565 1706-1709 NN denotes DNA
T2566 1710-1718 VBN denotes digested
T2567 1719-1721 IN denotes by
T2568 1722-1727 NN denotes BamHI
T2569 1728-1731 CC denotes and
T2570 1732-1733 DT denotes a
T2571 1734-1739 NN denotes probe
T2572 1740-1750 VBZ denotes comprising
T2573 1751-1753 IN denotes of
T2574 1754-1755 DT denotes a
T2576 1756-1759 CD denotes 562
T2577 1760-1762 NN denotes bp
T2578 1763-1766 NN denotes PCR
T2575 1767-1775 NN denotes fragment
T2579 1776-1780 IN denotes from
T2580 1781-1788 JJ denotes partial
T2581 1789-1795 NN denotes intron
T2582 1796-1797 CD denotes 2
T2583 1798-1801 CC denotes and
T2584 1802-1806 NN denotes exon
T2585 1807-1808 CD denotes 3
T2586 1809-1811 IN denotes of
T2587 1812-1817 NN denotes Abcg8
T2588 1818-1822 NN denotes gene
T2589 1822-1823 . denotes .
T2590 1823-1944 sentence denotes Positively targeted ES cells were microinjected into C57BL6 blastocysts and transplanted into pseudopregnant recipients.
T2591 1824-1834 RB denotes Positively
T2592 1835-1843 VBN denotes targeted
T2594 1844-1846 NN denotes ES
T2593 1847-1852 NNS denotes cells
T2596 1853-1857 VBD denotes were
T2595 1858-1871 VBN denotes microinjected
T2597 1872-1876 IN denotes into
T2598 1877-1883 NN denotes C57BL6
T2599 1884-1895 NNS denotes blastocysts
T2600 1896-1899 CC denotes and
T2601 1900-1912 VBN denotes transplanted
T2602 1913-1917 IN denotes into
T2603 1918-1932 JJ denotes pseudopregnant
T2604 1933-1943 NNS denotes recipients
T2605 1943-1944 . denotes .
T2606 1944-2140 sentence denotes Five highly chimeric mice (agouti coat color, three males and two females) were isolated and bred with C57BL/6J mice to generate germ-line transmission heterozygous mice of Abcg8 gene disruption.
T2607 1945-1949 CD denotes Five
T2609 1950-1956 RB denotes highly
T2610 1957-1965 JJ denotes chimeric
T2608 1966-1970 NNS denotes mice
T2612 1971-1972 -LRB- denotes (
T2614 1972-1978 JJ denotes agouti
T2616 1979-1983 NN denotes coat
T2615 1984-1989 NN denotes color
T2617 1989-1991 , denotes ,
T2618 1991-1996 CD denotes three
T2613 1997-2002 NNS denotes males
T2619 2003-2006 CC denotes and
T2620 2007-2010 CD denotes two
T2621 2011-2018 NNS denotes females
T2622 2018-2019 -RRB- denotes )
T2623 2020-2024 VBD denotes were
T2611 2025-2033 VBN denotes isolated
T2624 2034-2037 CC denotes and
T2625 2038-2042 VBN denotes bred
T2626 2043-2047 IN denotes with
T2627 2048-2053 NN denotes C57BL
T2629 2053-2054 HYPH denotes /
T2628 2054-2056 NN denotes 6J
T2630 2057-2061 NNS denotes mice
T2631 2062-2064 TO denotes to
T2632 2065-2073 VB denotes generate
T2633 2074-2078 NN denotes germ
T2635 2078-2079 HYPH denotes -
T2634 2079-2083 NN denotes line
T2636 2084-2096 NN denotes transmission
T2637 2097-2109 JJ denotes heterozygous
T2638 2110-2114 NNS denotes mice
T2639 2115-2117 IN denotes of
T2640 2118-2123 NN denotes Abcg8
T2641 2124-2128 NN denotes gene
T2642 2129-2139 NN denotes disruption
T2643 2139-2140 . denotes .
T2644 2140-2282 sentence denotes Heterozygous offspring mice were back-crossed to C57BL/6J mice (n > 5) to produce disrupted line and inter-crossed to generate knockout mice.
T2645 2141-2153 JJ denotes Heterozygous
T2647 2154-2163 NN denotes offspring
T2646 2164-2168 NNS denotes mice
T2649 2169-2173 VBD denotes were
T2650 2174-2178 RB denotes back
T2651 2178-2179 HYPH denotes -
T2648 2179-2186 VBN denotes crossed
T2652 2187-2189 IN denotes to
T2653 2190-2195 NN denotes C57BL
T2655 2195-2196 HYPH denotes /
T2654 2196-2198 NN denotes 6J
T2656 2199-2203 NNS denotes mice
T2657 2204-2205 -LRB- denotes (
T2659 2205-2206 NN denotes n
T2660 2207-2208 SYM denotes >
T2658 2209-2210 CD denotes 5
T2661 2210-2211 -RRB- denotes )
T2662 2212-2214 TO denotes to
T2663 2215-2222 VB denotes produce
T2664 2223-2232 VBN denotes disrupted
T2665 2233-2237 NN denotes line
T2666 2238-2241 CC denotes and
T2667 2242-2255 VBN denotes inter-crossed
T2668 2256-2258 TO denotes to
T2669 2259-2267 VB denotes generate
T2670 2268-2276 NN denotes knockout
T2671 2277-2281 NNS denotes mice
T2672 2281-2282 . denotes .
R1242 T2220 T2221 compound Targeting,vector
R1243 T2221 T2222 compound vector,construction
R1244 T2223 T2222 cc and,construction
R1245 T2224 T2222 conj generation,construction
R1246 T2225 T2224 prep of,generation
R1247 T2226 T2227 compound knockout,mice
R1248 T2227 T2225 pobj mice,of
R1249 T2229 T2230 det A,library
R1250 T2230 T2239 nsubjpass library,screened
R1251 T2231 T2232 npadvmod mouse,genomic
R1252 T2232 T2230 amod genomic,library
R1253 T2233 T2234 amod bacterial,chromosome
R1254 T2234 T2230 nmod chromosome,library
R1255 T2235 T2234 amod artificial,chromosome
R1256 T2236 T2234 punct (,chromosome
R1257 T2237 T2234 appos BAC,chromosome
R1258 T2238 T2230 punct ),library
R1259 T2240 T2241 punct (,Huntsville
R1260 T2241 T2230 parataxis Huntsville,library
R1261 T2242 T2241 dep CitbCJ7,Huntsville
R1262 T2243 T2241 punct ", ",Huntsville
R1263 T2244 T2245 compound ES,cell
R1264 T2245 T2246 compound cell,129Sv
R1265 T2246 T2241 dep 129Sv,Huntsville
R1266 T2247 T2246 compound line,129Sv
R1267 T2248 T2246 punct /,129Sv
R1268 T2249 T2241 punct ", ",Huntsville
R1269 T2250 T2251 compound Research,Genetics
R1270 T2251 T2241 dep Genetics,Huntsville
R1271 T2252 T2251 punct ", ",Genetics
R1272 T2253 T2251 amod Inc.,Genetics
R1273 T2254 T2241 punct ", ",Huntsville
R1274 T2255 T2241 punct ", ",Huntsville
R1275 T2256 T2241 npadvmod AL,Huntsville
R1276 T2257 T2241 punct ", ",Huntsville
R1277 T2258 T2241 npadvmod USA,Huntsville
R1278 T2259 T2241 punct ),Huntsville
R1279 T2260 T2239 auxpass was,screened
R1280 T2261 T2239 prep by,screened
R1281 T2262 T2261 pcomp using,by
R1282 T2263 T2262 dobj primers,using
R1283 T2264 T2263 acl designed,primers
R1284 T2265 T2264 prep from,designed
R1285 T2266 T2267 det the,sequences
R1286 T2267 T2265 pobj sequences,from
R1287 T2268 T2267 prep of,sequences
R1288 T2269 T2270 nmod mouse,cDNA
R1289 T2270 T2268 pobj cDNA,of
R1290 T2271 T2270 nmod Abcg5,cDNA
R1291 T2272 T2271 cc and,Abcg5
R1292 T2273 T2271 conj Abcg8,Abcg5
R1293 T2274 T2275 mark as,reported
R1294 T2275 T2262 advcl reported,using
R1295 T2276 T2275 advmod previously,reported
R1296 T2277 T2278 punct [,21
R1297 T2278 T2239 parataxis 21,screened
R1298 T2279 T2278 punct ],21
R1299 T2280 T2239 punct .,screened
R1300 T2282 T2283 det A,clone
R1301 T2283 T2286 nsubjpass clone,used
R1302 T2284 T2283 amod positive,clone
R1303 T2285 T2283 compound BAC,clone
R1304 T2287 T2286 auxpass was,used
R1305 T2288 T2286 prep as,used
R1306 T2289 T2290 det a,template
R1307 T2290 T2288 pobj template,as
R1308 T2291 T2292 aux to,amplify
R1309 T2292 T2286 advcl amplify,used
R1310 T2293 T2294 amod genomic,fragments
R1311 T2294 T2292 dobj fragments,amplify
R1312 T2295 T2294 compound DNA,fragments
R1313 T2296 T2294 prep of,fragments
R1314 T2297 T2296 pobj Abcg8,of
R1315 T2298 T2286 punct .,used
R1316 T2300 T2301 amod Long,fragment
R1317 T2301 T2303 nsubjpass fragment,performed
R1318 T2302 T2301 punct -,fragment
R1319 T2304 T2305 compound polymerase,reaction
R1320 T2305 T2301 appos reaction,fragment
R1321 T2306 T2305 compound chain,reaction
R1322 T2307 T2305 punct (,reaction
R1323 T2308 T2305 appos PCR,reaction
R1324 T2309 T2303 punct ),performed
R1325 T2310 T2303 auxpass was,performed
R1326 T2311 T2303 advcl using,performed
R1327 T2312 T2313 nmod Expanded,system
R1328 T2313 T2317 compound system,kit
R1329 T2314 T2315 amod Long,PCR
R1330 T2315 T2313 compound PCR,system
R1331 T2316 T2315 compound Template,PCR
R1332 T2317 T2311 dobj kit,using
R1333 T2318 T2319 punct (,Science
R1334 T2319 T2317 parataxis Science,kit
R1335 T2320 T2319 compound Roche,Science
R1336 T2321 T2319 compound Applied,Science
R1337 T2322 T2319 punct ", ",Science
R1338 T2323 T2319 npadvmod Indianapolis,Science
R1339 T2324 T2319 punct ", ",Science
R1340 T2325 T2319 npadvmod IA,Science
R1341 T2326 T2319 punct ", ",Science
R1342 T2327 T2319 npadvmod USA,Science
R1343 T2328 T2319 punct ),Science
R1344 T2329 T2303 punct .,performed
R1345 T2331 T2332 det An,fragment
R1346 T2332 T2342 nsubjpass fragment,inserted
R1347 T2333 T2334 advmod approximately,4.5
R1348 T2334 T2335 nummod 4.5,kb
R1349 T2335 T2332 nmod kb,fragment
R1350 T2336 T2332 punct ',fragment
R1351 T2337 T2338 amod long,arm
R1352 T2338 T2332 nmod arm,fragment
R1353 T2339 T2338 punct -,arm
R1354 T2340 T2332 punct ',fragment
R1355 T2341 T2332 amod genomic,fragment
R1356 T2343 T2332 acl containing,fragment
R1357 T2344 T2345 amod partial,exon
R1358 T2345 T2343 dobj exon,containing
R1359 T2346 T2345 nummod 1,exon
R1360 T2347 T2345 prep to,exon
R1361 T2348 T2349 amod partial,exon
R1362 T2349 T2347 pobj exon,to
R1363 T2350 T2349 nummod 3,exon
R1364 T2351 T2342 auxpass was,inserted
R1365 T2352 T2342 prep into,inserted
R1366 T2353 T2354 det the,A
R1367 T2354 T2352 pobj A,into
R1368 T2355 T2354 nmod Pml,A
R1369 T2356 T2355 nummod I,Pml
R1370 T2357 T2358 npadvmod restriction,cloning
R1371 T2358 T2354 amod cloning,A
R1372 T2359 T2358 punct -,cloning
R1373 T2360 T2354 compound site,A
R1374 T2361 T2354 prep of,A
R1375 T2362 T2363 compound OSDUPDEL,vector
R1376 T2363 T2361 pobj vector,of
R1377 T2364 T2342 punct .,inserted
R1378 T2366 T2367 det The,fragment
R1379 T2367 T2374 nsubjpass fragment,cloned
R1380 T2368 T2367 punct ',fragment
R1381 T2369 T2370 nmod short,arm
R1382 T2370 T2367 nmod arm,fragment
R1383 T2371 T2370 punct -,arm
R1384 T2372 T2367 punct ',fragment
R1385 T2373 T2367 amod genomic,fragment
R1386 T2375 T2367 punct ", ",fragment
R1387 T2376 T2367 acl containing,fragment
R1388 T2377 T2378 amod partial,exon
R1389 T2378 T2376 dobj exon,containing
R1390 T2379 T2378 nummod 4,exon
R1391 T2380 T2378 prep to,exon
R1392 T2381 T2382 amod partial,exon
R1393 T2382 T2380 pobj exon,to
R1394 T2383 T2382 nummod 6,exon
R1395 T2384 T2374 punct ", ",cloned
R1396 T2385 T2374 auxpass was,cloned
R1397 T2386 T2374 prep into,cloned
R1398 T2387 T2388 det the,Kpn
R1399 T2388 T2386 pobj Kpn,into
R1400 T2389 T2390 compound Not,I
R1401 T2390 T2388 compound I,Kpn
R1402 T2391 T2388 punct -,Kpn
R1403 T2392 T2388 nummod I,Kpn
R1404 T2393 T2388 prep of,Kpn
R1405 T2394 T2395 amod cloning,B
R1406 T2395 T2393 pobj B,of
R1407 T2396 T2395 compound site,B
R1408 T2397 T2374 punct .,cloned
R1409 T2399 T2400 amod Homologous,targeting
R1410 T2400 T2401 nsubj targeting,result
R1411 T2402 T2401 aux would,result
R1412 T2403 T2401 prep in,result
R1413 T2404 T2405 amod complete,intron
R1414 T2405 T2403 pobj intron,in
R1415 T2406 T2405 nummod 3,intron
R1416 T2407 T2405 punct ", ",intron
R1417 T2408 T2409 amod partial,exon
R1418 T2409 T2405 conj exon,intron
R1419 T2410 T2409 nummod 3,exon
R1420 T2411 T2409 cc and,exon
R1421 T2412 T2413 amod partial,exon
R1422 T2413 T2414 nmod exon,replacement
R1423 T2414 T2409 conj replacement,exon
R1424 T2415 T2413 nummod 4,exon
R1425 T2416 T2405 prep by,intron
R1426 T2417 T2418 det the,cassette
R1427 T2418 T2416 pobj cassette,by
R1428 T2419 T2418 nmod neomycin,cassette
R1429 T2420 T2419 punct -,neomycin
R1430 T2421 T2419 amod resistance,neomycin
R1431 T2422 T2401 punct ", ",result
R1432 T2423 T2401 advcl resulting,result
R1433 T2424 T2423 prep in,resulting
R1434 T2425 T2426 det the,disruption
R1435 T2426 T2424 pobj disruption,in
R1436 T2427 T2426 prep of,disruption
R1437 T2428 T2429 det the,motif
R1438 T2429 T2427 pobj motif,of
R1439 T2430 T2431 compound ABC,A
R1440 T2431 T2429 compound A,motif
R1441 T2432 T2431 compound Walker,A
R1442 T2433 T2434 punct (,1a
R1443 T2434 T2401 parataxis 1a,result
R1444 T2435 T2434 compound Figure,1a
R1445 T2436 T2434 punct ),1a
R1446 T2437 T2401 punct .,result
R1447 T2439 T2440 compound ES,cells
R1448 T2440 T2441 nsubjpass cells,electroporated
R1449 T2442 T2443 punct (,line
R1450 T2443 T2440 parataxis line,cells
R1451 T2444 T2445 nummod 129,SvEvTac
R1452 T2445 T2443 compound SvEvTac,line
R1453 T2446 T2445 punct /,SvEvTac
R1454 T2447 T2443 punct -,line
R1455 T2448 T2443 compound cell,line
R1456 T2449 T2443 punct ),line
R1457 T2450 T2441 auxpass were,electroporated
R1458 T2451 T2441 prep with,electroporated
R1459 T2452 T2453 det the,DNA
R1460 T2453 T2451 pobj DNA,with
R1461 T2454 T2455 amod linearized,vector
R1462 T2455 T2453 compound vector,DNA
R1463 T2456 T2455 compound targeting,vector
R1464 T2457 T2441 cc and,electroporated
R1465 T2458 T2441 conj cultured,electroporated
R1466 T2459 T2458 prep on,cultured
R1467 T2460 T2461 amod sub-confluent,fibroblasts
R1468 T2461 T2459 pobj fibroblasts,on
R1469 T2462 T2461 amod embryonic,fibroblasts
R1470 T2463 T2441 punct .,electroporated
R1471 T2465 T2466 amod Transfected,colonies
R1472 T2466 T2468 nsubjpass colonies,selected
R1473 T2467 T2466 compound cell,colonies
R1474 T2469 T2468 auxpass were,selected
R1475 T2470 T2468 agent by,selected
R1476 T2471 T2470 pcomp culturing,by
R1477 T2472 T2471 dobj cells,culturing
R1478 T2473 T2471 prep in,culturing
R1479 T2474 T2473 pobj medium,in
R1480 T2475 T2474 acl containing,medium
R1481 T2476 T2477 nummod 200,μg
R1482 T2477 T2478 nmod μg,G418
R1483 T2478 T2475 dobj G418,containing
R1484 T2479 T2480 punct /,mL
R1485 T2480 T2477 prep mL,μg
R1486 T2481 T2482 punct (,Technologies
R1487 T2482 T2478 parataxis Technologies,G418
R1488 T2483 T2482 compound Life,Technologies
R1489 T2484 T2482 punct ", ",Technologies
R1490 T2485 T2482 npadvmod Rockville,Technologies
R1491 T2486 T2482 punct ", ",Technologies
R1492 T2487 T2482 npadvmod MD,Technologies
R1493 T2488 T2482 punct ", ",Technologies
R1494 T2489 T2482 npadvmod USA,Technologies
R1495 T2490 T2482 punct ),Technologies
R1496 T2491 T2468 punct .,selected
R1497 T2493 T2494 det The,cells
R1498 T2494 T2496 nsubjpass cells,screened
R1499 T2495 T2494 compound ES,cells
R1500 T2497 T2496 auxpass were,screened
R1501 T2498 T2496 prep for,screened
R1502 T2499 T2500 amod homologous,recombination
R1503 T2500 T2498 pobj recombination,for
R1504 T2501 T2496 prep by,screened
R1505 T2502 T2501 pobj PCR,by
R1506 T2503 T2496 prep with,screened
R1507 T2504 T2505 det the,primer
R1508 T2505 T2503 pobj primer,with
R1509 T2506 T2505 amod forward,primer
R1510 T2507 T2505 punct ", ",primer
R1511 T2508 T2505 appos neoF,primer
R1512 T2509 T2508 punct (,neoF
R1513 T2510 T2511 nummod 5,GGGTCGTTTGTTCGGATCAA
R1514 T2511 T2508 appos GGGTCGTTTGTTCGGATCAA,neoF
R1515 T2512 T2511 punct ',GGGTCGTTTGTTCGGATCAA
R1516 T2513 T2511 punct -,GGGTCGTTTGTTCGGATCAA
R1517 T2514 T2511 punct -,GGGTCGTTTGTTCGGATCAA
R1518 T2515 T2511 nummod 3,GGGTCGTTTGTTCGGATCAA
R1519 T2516 T2511 punct ',GGGTCGTTTGTTCGGATCAA
R1520 T2517 T2508 punct ),neoF
R1521 T2518 T2508 prep from,neoF
R1522 T2519 T2520 compound neo,cassette
R1523 T2520 T2518 pobj cassette,from
R1524 T2521 T2505 punct ", ",primer
R1525 T2522 T2505 cc and,primer
R1526 T2523 T2524 amod reverse,primer
R1527 T2524 T2525 compound primer,6R
R1528 T2525 T2505 conj 6R,primer
R1529 T2526 T2525 compound intron,6R
R1530 T2527 T2525 punct (,6R
R1531 T2528 T2529 nummod 5,ACCAGTTGGTCCTAGCTCGA
R1532 T2529 T2525 appos ACCAGTTGGTCCTAGCTCGA,6R
R1533 T2530 T2528 punct ',5
R1534 T2531 T2529 punct -,ACCAGTTGGTCCTAGCTCGA
R1535 T2532 T2529 punct -,ACCAGTTGGTCCTAGCTCGA
R1536 T2533 T2529 nummod 3,ACCAGTTGGTCCTAGCTCGA
R1537 T2534 T2529 punct ',ACCAGTTGGTCCTAGCTCGA
R1538 T2535 T2525 punct ),6R
R1539 T2536 T2525 punct ", ",6R
R1540 T2537 T2538 dep which,located
R1541 T2538 T2525 relcl located,6R
R1542 T2539 T2538 auxpass is,located
R1543 T2540 T2538 prep outside,located
R1544 T2541 T2542 det the,construct
R1545 T2542 T2540 pobj construct,outside
R1546 T2543 T2542 amod targeting,construct
R1547 T2544 T2538 prep in,located
R1548 T2545 T2546 compound mouse,intron
R1549 T2546 T2544 pobj intron,in
R1550 T2547 T2546 compound Abcg8,intron
R1551 T2548 T2546 nummod 6,intron
R1552 T2549 T2496 punct .,screened
R1553 T2551 T2552 advmod Positively,targeted
R1554 T2552 T2553 amod targeted,clones
R1555 T2553 T2556 nsubjpass clones,confirmed
R1556 T2554 T2555 compound ES,cell
R1557 T2555 T2553 compound cell,clones
R1558 T2557 T2556 auxpass were,confirmed
R1559 T2558 T2556 prep by,confirmed
R1560 T2559 T2560 compound Southern,blotting
R1561 T2560 T2558 pobj blotting,by
R1562 T2561 T2556 punct ", ",confirmed
R1563 T2562 T2556 advcl using,confirmed
R1564 T2563 T2564 compound ES,cell
R1565 T2564 T2565 compound cell,DNA
R1566 T2565 T2562 dobj DNA,using
R1567 T2566 T2565 acl digested,DNA
R1568 T2567 T2566 prep by,digested
R1569 T2568 T2567 pobj BamHI,by
R1570 T2569 T2568 cc and,BamHI
R1571 T2570 T2571 det a,probe
R1572 T2571 T2568 conj probe,BamHI
R1573 T2572 T2571 acl comprising,probe
R1574 T2573 T2572 prep of,comprising
R1575 T2574 T2575 det a,fragment
R1576 T2575 T2573 pobj fragment,of
R1577 T2576 T2577 nummod 562,bp
R1578 T2577 T2575 compound bp,fragment
R1579 T2578 T2575 compound PCR,fragment
R1580 T2579 T2575 prep from,fragment
R1581 T2580 T2581 amod partial,intron
R1582 T2581 T2579 pobj intron,from
R1583 T2582 T2581 nummod 2,intron
R1584 T2583 T2581 cc and,intron
R1585 T2584 T2581 conj exon,intron
R1586 T2585 T2584 nummod 3,exon
R1587 T2586 T2581 prep of,intron
R1588 T2587 T2588 compound Abcg8,gene
R1589 T2588 T2586 pobj gene,of
R1590 T2589 T2556 punct .,confirmed
R1591 T2591 T2592 advmod Positively,targeted
R1592 T2592 T2593 amod targeted,cells
R1593 T2593 T2595 nsubjpass cells,microinjected
R1594 T2594 T2593 compound ES,cells
R1595 T2596 T2595 auxpass were,microinjected
R1596 T2597 T2595 prep into,microinjected
R1597 T2598 T2599 compound C57BL6,blastocysts
R1598 T2599 T2597 pobj blastocysts,into
R1599 T2600 T2595 cc and,microinjected
R1600 T2601 T2595 conj transplanted,microinjected
R1601 T2602 T2601 prep into,transplanted
R1602 T2603 T2604 amod pseudopregnant,recipients
R1603 T2604 T2602 pobj recipients,into
R1604 T2605 T2595 punct .,microinjected
R1605 T2607 T2608 nummod Five,mice
R1606 T2608 T2611 nsubjpass mice,isolated
R1607 T2609 T2610 advmod highly,chimeric
R1608 T2610 T2608 amod chimeric,mice
R1609 T2612 T2613 punct (,males
R1610 T2613 T2608 parataxis males,mice
R1611 T2614 T2615 amod agouti,color
R1612 T2615 T2613 dep color,males
R1613 T2616 T2615 compound coat,color
R1614 T2617 T2613 punct ", ",males
R1615 T2618 T2613 nummod three,males
R1616 T2619 T2613 cc and,males
R1617 T2620 T2621 nummod two,females
R1618 T2621 T2613 conj females,males
R1619 T2622 T2613 punct ),males
R1620 T2623 T2611 auxpass were,isolated
R1621 T2624 T2611 cc and,isolated
R1622 T2625 T2611 conj bred,isolated
R1623 T2626 T2625 prep with,bred
R1624 T2627 T2628 compound C57BL,6J
R1625 T2628 T2630 compound 6J,mice
R1626 T2629 T2628 punct /,6J
R1627 T2630 T2626 pobj mice,with
R1628 T2631 T2632 aux to,generate
R1629 T2632 T2625 advcl generate,bred
R1630 T2633 T2634 compound germ,line
R1631 T2634 T2636 compound line,transmission
R1632 T2635 T2634 punct -,line
R1633 T2636 T2637 npadvmod transmission,heterozygous
R1634 T2637 T2638 amod heterozygous,mice
R1635 T2638 T2632 dobj mice,generate
R1636 T2639 T2638 prep of,mice
R1637 T2640 T2641 compound Abcg8,gene
R1638 T2641 T2642 compound gene,disruption
R1639 T2642 T2639 pobj disruption,of
R1640 T2643 T2611 punct .,isolated
R1641 T2645 T2646 amod Heterozygous,mice
R1642 T2646 T2648 nsubjpass mice,crossed
R1643 T2647 T2646 compound offspring,mice
R1644 T2649 T2648 auxpass were,crossed
R1645 T2650 T2648 advmod back,crossed
R1646 T2651 T2648 punct -,crossed
R1647 T2652 T2648 prep to,crossed
R1648 T2653 T2654 compound C57BL,6J
R1649 T2654 T2656 compound 6J,mice
R1650 T2655 T2654 punct /,6J
R1651 T2656 T2652 pobj mice,to
R1652 T2657 T2658 punct (,5
R1653 T2658 T2656 parataxis 5,mice
R1654 T2659 T2658 nsubj n,5
R1655 T2660 T2658 punct >,5
R1656 T2661 T2658 punct ),5
R1657 T2662 T2663 aux to,produce
R1658 T2663 T2648 advcl produce,crossed
R1659 T2664 T2665 amod disrupted,line
R1660 T2665 T2663 dobj line,produce
R1661 T2666 T2648 cc and,crossed
R1662 T2667 T2648 conj inter-crossed,crossed
R1663 T2668 T2669 aux to,generate
R1664 T2669 T2667 advcl generate,inter-crossed
R1665 T2670 T2671 compound knockout,mice
R1666 T2671 T2669 dobj mice,generate
R1667 T2672 T2648 punct .,crossed