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PMC:394351 / 19312-21001 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T5424 0-19 JJ denotes Immunohistochemical
T5425 20-28 NN denotes analysis
T5426 28-194 sentence denotes Snap-frozen liver and intestine tissue were cut to produce 8 μm thick frozen sections, air-dried for 30 minutes onto glass slides and stored at -80°C until required.
T5427 29-33 RB denotes Snap
T5429 33-34 HYPH denotes -
T5428 34-40 VBN denotes frozen
T5431 41-46 NN denotes liver
T5432 47-50 CC denotes and
T5433 51-60 NN denotes intestine
T5430 61-67 NN denotes tissue
T5435 68-72 VBD denotes were
T5434 73-76 VBN denotes cut
T5436 77-79 TO denotes to
T5437 80-87 VB denotes produce
T5438 88-89 CD denotes 8
T5439 90-92 NN denotes μm
T5440 93-98 JJ denotes thick
T5442 99-105 JJ denotes frozen
T5441 106-114 NNS denotes sections
T5443 114-116 , denotes ,
T5444 116-119 NN denotes air
T5446 119-120 HYPH denotes -
T5445 120-125 VBN denotes dried
T5447 126-129 IN denotes for
T5448 130-132 CD denotes 30
T5449 133-140 NNS denotes minutes
T5450 141-145 IN denotes onto
T5451 146-151 NN denotes glass
T5452 152-158 NNS denotes slides
T5453 159-162 CC denotes and
T5454 163-169 VBN denotes stored
T5455 170-172 IN denotes at
T5456 173-174 SYM denotes -
T5457 174-176 CD denotes 80
T5458 176-178 NN denotes °C
T5459 179-184 IN denotes until
T5460 185-193 VBN denotes required
T5461 193-194 . denotes .
T5462 194-350 sentence denotes The slides were stained with hematoxylin, rinsed three times with PBS, fixed for 10 min with cooled methanol at -20°C and then rinsed three times with PBS.
T5463 195-198 DT denotes The
T5464 199-205 NNS denotes slides
T5466 206-210 VBD denotes were
T5465 211-218 VBN denotes stained
T5467 219-223 IN denotes with
T5468 224-235 NN denotes hematoxylin
T5469 235-237 , denotes ,
T5470 237-243 VBD denotes rinsed
T5471 244-249 CD denotes three
T5472 250-255 NNS denotes times
T5473 256-260 IN denotes with
T5474 261-264 NN denotes PBS
T5475 264-266 , denotes ,
T5476 266-271 VBN denotes fixed
T5477 272-275 IN denotes for
T5478 276-278 CD denotes 10
T5479 279-282 NN denotes min
T5480 283-287 IN denotes with
T5481 288-294 VBN denotes cooled
T5482 295-303 NN denotes methanol
T5483 304-306 IN denotes at
T5484 307-308 SYM denotes -
T5485 308-310 CD denotes 20
T5486 310-312 NN denotes °C
T5487 313-316 CC denotes and
T5488 317-321 RB denotes then
T5489 322-328 VBD denotes rinsed
T5490 329-334 CD denotes three
T5491 335-340 NNS denotes times
T5492 341-345 IN denotes with
T5493 346-349 NN denotes PBS
T5494 349-350 . denotes .
T5495 350-548 sentence denotes Slides for antibody staining were initially blocked in PBS/0.1 M glycine/10% goat serum for 60 min at room temperature then incubated with anti-sterolin-1 antibody (1:15 dilution) overnight at 4°C.
T5496 351-357 NNS denotes Slides
T5498 358-361 IN denotes for
T5499 362-370 NN denotes antibody
T5500 371-379 NN denotes staining
T5501 380-384 VBD denotes were
T5502 385-394 RB denotes initially
T5497 395-402 VBN denotes blocked
T5503 403-405 IN denotes in
T5504 406-409 NN denotes PBS
T5506 409-410 HYPH denotes /
T5507 410-413 CD denotes 0.1
T5505 414-415 NN denotes M
T5509 416-423 NN denotes glycine
T5511 423-424 HYPH denotes /
T5512 424-426 CD denotes 10
T5510 426-427 NN denotes %
T5513 428-432 NN denotes goat
T5508 433-438 NN denotes serum
T5514 439-442 IN denotes for
T5515 443-445 CD denotes 60
T5516 446-449 NN denotes min
T5517 450-452 IN denotes at
T5518 453-457 NN denotes room
T5519 458-469 NN denotes temperature
T5520 470-474 RB denotes then
T5521 475-484 VBN denotes incubated
T5522 485-489 IN denotes with
T5523 490-503 JJ denotes anti-sterolin
T5525 503-504 HYPH denotes -
T5526 504-505 CD denotes 1
T5524 506-514 NN denotes antibody
T5527 515-516 -LRB- denotes (
T5529 516-517 CD denotes 1
T5530 517-518 SYM denotes :
T5531 518-520 CD denotes 15
T5528 521-529 NN denotes dilution
T5532 529-530 -RRB- denotes )
T5533 531-540 RB denotes overnight
T5534 541-543 IN denotes at
T5535 544-545 CD denotes 4
T5536 545-547 NN denotes °C
T5537 547-548 . denotes .
T5538 548-802 sentence denotes The slides were washed with 1 × PBS and incubated with secondary antibody (goat-anti-rabbit IgG conjugated with rhodamine, 1:1000 dilution) for 30 min at room temperature, washed and then observed under an Olympus BX-5 confocal microscope with Fluoview.
T5539 549-552 DT denotes The
T5540 553-559 NNS denotes slides
T5542 560-564 VBD denotes were
T5541 565-571 VBN denotes washed
T5543 572-576 IN denotes with
T5544 577-578 CD denotes 1
T5546 579-580 SYM denotes ×
T5545 581-584 NN denotes PBS
T5547 585-588 CC denotes and
T5548 589-598 VBN denotes incubated
T5549 599-603 IN denotes with
T5550 604-613 JJ denotes secondary
T5551 614-622 NN denotes antibody
T5552 623-624 -LRB- denotes (
T5554 624-628 NN denotes goat
T5556 628-629 HYPH denotes -
T5555 629-640 NN denotes anti-rabbit
T5557 641-644 NN denotes IgG
T5558 645-655 VBN denotes conjugated
T5559 656-660 IN denotes with
T5560 661-670 NN denotes rhodamine
T5561 670-672 , denotes ,
T5562 672-673 CD denotes 1
T5563 673-674 SYM denotes :
T5564 674-678 CD denotes 1000
T5553 679-687 NN denotes dilution
T5565 687-688 -RRB- denotes )
T5566 689-692 IN denotes for
T5567 693-695 CD denotes 30
T5568 696-699 NN denotes min
T5569 700-702 IN denotes at
T5570 703-707 NN denotes room
T5571 708-719 NN denotes temperature
T5572 719-721 , denotes ,
T5573 721-727 VBD denotes washed
T5574 728-731 CC denotes and
T5575 732-736 RB denotes then
T5576 737-745 VBN denotes observed
T5577 746-751 IN denotes under
T5578 752-754 DT denotes an
T5580 755-762 NNP denotes Olympus
T5581 763-765 NN denotes BX
T5582 765-766 HYPH denotes -
T5583 766-767 CD denotes 5
T5584 768-776 JJ denotes confocal
T5579 777-787 NN denotes microscope
T5585 788-792 IN denotes with
T5586 793-801 NNP denotes Fluoview
T5587 801-802 . denotes .
T5588 802-1105 sentence denotes The sections stained at the AMC Liver Center were fixed in 100% acetone for 30 min at room temperature, blocked in a solution of PBS/0.05% Tween-20/5% goat serum (GS) for one hour, then incubated with Anti-sterolin-1 (1:40 dilution) and anti-Bsep (1:200 dilution) diluted in PBS/Tween/GS, for one hour.
T5589 803-806 DT denotes The
T5590 807-815 NNS denotes sections
T5592 816-823 VBN denotes stained
T5593 824-826 IN denotes at
T5594 827-830 DT denotes the
T5596 831-834 NNP denotes AMC
T5597 835-840 NNP denotes Liver
T5595 841-847 NNP denotes Center
T5598 848-852 VBD denotes were
T5591 853-858 VBN denotes fixed
T5599 859-861 IN denotes in
T5600 862-865 CD denotes 100
T5601 865-866 NN denotes %
T5602 867-874 NN denotes acetone
T5603 875-878 IN denotes for
T5604 879-881 CD denotes 30
T5605 882-885 NN denotes min
T5606 886-888 IN denotes at
T5607 889-893 NN denotes room
T5608 894-905 NN denotes temperature
T5609 905-907 , denotes ,
T5610 907-914 VBD denotes blocked
T5611 915-917 IN denotes in
T5612 918-919 DT denotes a
T5613 920-928 NN denotes solution
T5614 929-931 IN denotes of
T5615 932-935 NN denotes PBS
T5617 935-936 HYPH denotes /
T5618 936-940 CD denotes 0.05
T5616 940-941 NN denotes %
T5619 942-947 NNP denotes Tween
T5621 947-948 HYPH denotes -
T5622 948-950 CD denotes 20
T5623 950-951 HYPH denotes /
T5624 951-952 CD denotes 5
T5620 952-953 NN denotes %
T5625 954-958 NN denotes goat
T5626 959-964 NN denotes serum
T5627 965-966 -LRB- denotes (
T5628 966-968 NN denotes GS
T5629 968-969 -RRB- denotes )
T5630 970-973 IN denotes for
T5631 974-977 CD denotes one
T5632 978-982 NN denotes hour
T5633 982-984 , denotes ,
T5634 984-988 RB denotes then
T5635 989-998 VBN denotes incubated
T5636 999-1003 IN denotes with
T5637 1004-1017 NN denotes Anti-sterolin
T5638 1017-1018 HYPH denotes -
T5639 1018-1019 CD denotes 1
T5640 1020-1021 -LRB- denotes (
T5642 1021-1022 CD denotes 1
T5643 1022-1023 SYM denotes :
T5644 1023-1025 CD denotes 40
T5641 1026-1034 NN denotes dilution
T5645 1034-1035 -RRB- denotes )
T5646 1036-1039 CC denotes and
T5647 1040-1049 JJ denotes anti-Bsep
T5648 1050-1051 -LRB- denotes (
T5650 1051-1052 CD denotes 1
T5651 1052-1053 SYM denotes :
T5652 1053-1056 CD denotes 200
T5649 1057-1065 NN denotes dilution
T5653 1065-1066 -RRB- denotes )
T5654 1067-1074 VBN denotes diluted
T5655 1075-1077 IN denotes in
T5656 1078-1081 NN denotes PBS
T5658 1081-1082 HYPH denotes /
T5659 1082-1087 NN denotes Tween
T5660 1087-1088 HYPH denotes /
T5657 1088-1090 NN denotes GS
T5661 1090-1092 , denotes ,
T5662 1092-1095 IN denotes for
T5663 1096-1099 CD denotes one
T5664 1100-1104 NN denotes hour
T5665 1104-1105 . denotes .
T5666 1105-1292 sentence denotes Thereafter, slides were washed 3 × 10 min in PBS/Tween and the secondary antibody (goat-anti-rabbit Alexa 488) was applied in 1:1000 dilution in PBST/GS for one hour at room temperature.
T5667 1106-1116 RB denotes Thereafter
T5669 1116-1118 , denotes ,
T5670 1118-1124 NNS denotes slides
T5671 1125-1129 VBD denotes were
T5668 1130-1136 VBN denotes washed
T5672 1137-1138 CD denotes 3
T5674 1139-1140 SYM denotes ×
T5675 1141-1143 CD denotes 10
T5673 1144-1147 NN denotes min
T5676 1148-1150 IN denotes in
T5677 1151-1154 NN denotes PBS
T5679 1154-1155 HYPH denotes /
T5678 1155-1160 NN denotes Tween
T5680 1161-1164 CC denotes and
T5681 1165-1168 DT denotes the
T5683 1169-1178 JJ denotes secondary
T5682 1179-1187 NN denotes antibody
T5685 1188-1189 -LRB- denotes (
T5686 1189-1193 NN denotes goat
T5688 1193-1194 HYPH denotes -
T5687 1194-1205 NN denotes anti-rabbit
T5689 1206-1211 NNP denotes Alexa
T5690 1212-1215 CD denotes 488
T5691 1215-1216 -RRB- denotes )
T5692 1217-1220 VBD denotes was
T5684 1221-1228 VBN denotes applied
T5693 1229-1231 IN denotes in
T5694 1232-1233 CD denotes 1
T5696 1233-1234 SYM denotes :
T5697 1234-1238 CD denotes 1000
T5695 1239-1247 NN denotes dilution
T5698 1248-1250 IN denotes in
T5699 1251-1255 NN denotes PBST
T5701 1255-1256 HYPH denotes /
T5700 1256-1258 NN denotes GS
T5702 1259-1262 IN denotes for
T5703 1263-1266 CD denotes one
T5704 1267-1271 NN denotes hour
T5705 1272-1274 IN denotes at
T5706 1275-1279 NN denotes room
T5707 1280-1291 NN denotes temperature
T5708 1291-1292 . denotes .
T5709 1292-1371 sentence denotes After washing for 3 × 10 min the sections were mounted in Dapi/Dapco solution.
T5710 1293-1298 IN denotes After
T5712 1299-1306 VBG denotes washing
T5713 1307-1310 IN denotes for
T5714 1311-1312 CD denotes 3
T5716 1313-1314 SYM denotes ×
T5717 1315-1317 CD denotes 10
T5715 1318-1321 NN denotes min
T5718 1322-1325 DT denotes the
T5719 1326-1334 NNS denotes sections
T5720 1335-1339 VBD denotes were
T5711 1340-1347 VBN denotes mounted
T5721 1348-1350 IN denotes in
T5722 1351-1355 NN denotes Dapi
T5724 1355-1356 HYPH denotes /
T5723 1356-1361 NN denotes Dapco
T5725 1362-1370 NN denotes solution
T5726 1370-1371 . denotes .
T5727 1371-1478 sentence denotes Fluorescence microscopy was done with a Leica DM RA2 microscope equipped with a Leica DC350F photo camera.
T5728 1372-1384 NN denotes Fluorescence
T5729 1385-1395 NN denotes microscopy
T5731 1396-1399 VBD denotes was
T5730 1400-1404 VBN denotes done
T5732 1405-1409 IN denotes with
T5733 1410-1411 DT denotes a
T5735 1412-1417 NNP denotes Leica
T5736 1418-1420 NN denotes DM
T5737 1421-1424 NN denotes RA2
T5734 1425-1435 NN denotes microscope
T5738 1436-1444 VBN denotes equipped
T5739 1445-1449 IN denotes with
T5740 1450-1451 DT denotes a
T5742 1452-1457 NNP denotes Leica
T5743 1458-1464 NN denotes DC350F
T5744 1465-1470 NN denotes photo
T5741 1471-1477 NN denotes camera
T5745 1477-1478 . denotes .
T5746 1478-1689 sentence denotes For Dapi staining, 359 nm (30.38 ms exposure) was used and for Abcg5 and Bsep staining, 514 nm (for both: 1654.34 ms exposure) was used and the pictures were analyzed and overlaid using FW4000 software (Leica).
T5747 1479-1482 IN denotes For
T5749 1483-1487 NNP denotes Dapi
T5750 1488-1496 NN denotes staining
T5751 1496-1498 , denotes ,
T5752 1498-1501 CD denotes 359
T5753 1502-1504 NN denotes nm
T5754 1505-1506 -LRB- denotes (
T5756 1506-1511 CD denotes 30.38
T5757 1512-1514 NN denotes ms
T5755 1515-1523 NN denotes exposure
T5758 1523-1524 -RRB- denotes )
T5759 1525-1528 VBD denotes was
T5748 1529-1533 VBN denotes used
T5760 1534-1537 CC denotes and
T5761 1538-1541 IN denotes for
T5763 1542-1547 NN denotes Abcg5
T5765 1548-1551 CC denotes and
T5766 1552-1556 NN denotes Bsep
T5764 1557-1565 NN denotes staining
T5767 1565-1567 , denotes ,
T5768 1567-1570 CD denotes 514
T5769 1571-1573 NN denotes nm
T5770 1574-1575 -LRB- denotes (
T5772 1575-1578 IN denotes for
T5773 1579-1583 DT denotes both
T5774 1583-1585 : denotes :
T5775 1585-1592 CD denotes 1654.34
T5776 1593-1595 NN denotes ms
T5771 1596-1604 NN denotes exposure
T5777 1604-1605 -RRB- denotes )
T5778 1606-1609 VBD denotes was
T5762 1610-1614 VBN denotes used
T5779 1615-1618 CC denotes and
T5780 1619-1622 DT denotes the
T5781 1623-1631 NNS denotes pictures
T5783 1632-1636 VBD denotes were
T5782 1637-1645 VBN denotes analyzed
T5784 1646-1649 CC denotes and
T5785 1650-1658 VBN denotes overlaid
T5786 1659-1664 VBG denotes using
T5787 1665-1671 NN denotes FW4000
T5788 1672-1680 NN denotes software
T5789 1681-1682 -LRB- denotes (
T5790 1682-1687 NNP denotes Leica
T5791 1687-1688 -RRB- denotes )
T5792 1688-1689 . denotes .
R3578 T5424 T5425 amod Immunohistochemical,analysis
R3579 T5427 T5428 advmod Snap,frozen
R3580 T5428 T5430 amod frozen,tissue
R3581 T5429 T5428 punct -,frozen
R3582 T5430 T5434 nsubjpass tissue,cut
R3583 T5431 T5430 nmod liver,tissue
R3584 T5432 T5431 cc and,liver
R3585 T5433 T5431 conj intestine,liver
R3586 T5435 T5434 auxpass were,cut
R3587 T5436 T5437 aux to,produce
R3588 T5437 T5434 advcl produce,cut
R3589 T5438 T5439 nummod 8,μm
R3590 T5439 T5440 npadvmod μm,thick
R3591 T5440 T5441 amod thick,sections
R3592 T5441 T5437 dobj sections,produce
R3593 T5442 T5441 amod frozen,sections
R3594 T5443 T5434 punct ", ",cut
R3595 T5444 T5445 dep air,dried
R3596 T5445 T5434 conj dried,cut
R3597 T5446 T5445 punct -,dried
R3598 T5447 T5445 prep for,dried
R3599 T5448 T5449 nummod 30,minutes
R3600 T5449 T5447 pobj minutes,for
R3601 T5450 T5445 prep onto,dried
R3602 T5451 T5452 compound glass,slides
R3603 T5452 T5450 pobj slides,onto
R3604 T5453 T5445 cc and,dried
R3605 T5454 T5445 conj stored,dried
R3606 T5455 T5454 prep at,stored
R3607 T5456 T5457 punct -,80
R3608 T5457 T5458 nummod 80,°C
R3609 T5458 T5455 pobj °C,at
R3610 T5459 T5460 mark until,required
R3611 T5460 T5454 advcl required,stored
R3612 T5461 T5434 punct .,cut
R3613 T5463 T5464 det The,slides
R3614 T5464 T5465 nsubjpass slides,stained
R3615 T5466 T5465 auxpass were,stained
R3616 T5467 T5465 prep with,stained
R3617 T5468 T5467 pobj hematoxylin,with
R3618 T5469 T5465 punct ", ",stained
R3619 T5470 T5465 conj rinsed,stained
R3620 T5471 T5472 nummod three,times
R3621 T5472 T5470 npadvmod times,rinsed
R3622 T5473 T5470 prep with,rinsed
R3623 T5474 T5473 pobj PBS,with
R3624 T5475 T5470 punct ", ",rinsed
R3625 T5476 T5470 conj fixed,rinsed
R3626 T5477 T5476 prep for,fixed
R3627 T5478 T5479 nummod 10,min
R3628 T5479 T5477 pobj min,for
R3629 T5480 T5476 prep with,fixed
R3630 T5481 T5482 amod cooled,methanol
R3631 T5482 T5480 pobj methanol,with
R3632 T5483 T5476 prep at,fixed
R3633 T5484 T5485 punct -,20
R3634 T5485 T5486 nummod 20,°C
R3635 T5486 T5483 pobj °C,at
R3636 T5487 T5476 cc and,fixed
R3637 T5488 T5489 advmod then,rinsed
R3638 T5489 T5476 conj rinsed,fixed
R3639 T5490 T5491 nummod three,times
R3640 T5491 T5489 npadvmod times,rinsed
R3641 T5492 T5489 prep with,rinsed
R3642 T5493 T5492 pobj PBS,with
R3643 T5494 T5465 punct .,stained
R3644 T5496 T5497 nsubjpass Slides,blocked
R3645 T5498 T5496 prep for,Slides
R3646 T5499 T5500 compound antibody,staining
R3647 T5500 T5498 pobj staining,for
R3648 T5501 T5497 auxpass were,blocked
R3649 T5502 T5497 advmod initially,blocked
R3650 T5503 T5497 prep in,blocked
R3651 T5504 T5505 nmod PBS,M
R3652 T5505 T5508 nmod M,serum
R3653 T5506 T5505 punct /,M
R3654 T5507 T5505 nummod 0.1,M
R3655 T5508 T5503 pobj serum,in
R3656 T5509 T5510 nmod glycine,%
R3657 T5510 T5508 compound %,serum
R3658 T5511 T5510 punct /,%
R3659 T5512 T5510 nummod 10,%
R3660 T5513 T5508 compound goat,serum
R3661 T5514 T5497 prep for,blocked
R3662 T5515 T5516 nummod 60,min
R3663 T5516 T5514 pobj min,for
R3664 T5517 T5497 prep at,blocked
R3665 T5518 T5519 compound room,temperature
R3666 T5519 T5517 pobj temperature,at
R3667 T5520 T5521 advmod then,incubated
R3668 T5521 T5497 dep incubated,blocked
R3669 T5522 T5521 prep with,incubated
R3670 T5523 T5524 amod anti-sterolin,antibody
R3671 T5524 T5522 pobj antibody,with
R3672 T5525 T5523 punct -,anti-sterolin
R3673 T5526 T5523 advmod 1,anti-sterolin
R3674 T5527 T5528 punct (,dilution
R3675 T5528 T5524 parataxis dilution,antibody
R3676 T5529 T5528 nummod 1,dilution
R3677 T5530 T5531 punct :,15
R3678 T5531 T5529 prep 15,1
R3679 T5532 T5528 punct ),dilution
R3680 T5533 T5521 advmod overnight,incubated
R3681 T5534 T5521 prep at,incubated
R3682 T5535 T5536 nummod 4,°C
R3683 T5536 T5534 pobj °C,at
R3684 T5537 T5497 punct .,blocked
R3685 T5539 T5540 det The,slides
R3686 T5540 T5541 nsubj slides,washed
R3687 T5542 T5541 aux were,washed
R3688 T5543 T5541 prep with,washed
R3689 T5544 T5545 nummod 1,PBS
R3690 T5545 T5543 pobj PBS,with
R3691 T5546 T5544 punct ×,1
R3692 T5547 T5541 cc and,washed
R3693 T5548 T5541 conj incubated,washed
R3694 T5549 T5548 prep with,incubated
R3695 T5550 T5551 amod secondary,antibody
R3696 T5551 T5549 pobj antibody,with
R3697 T5552 T5553 punct (,dilution
R3698 T5553 T5551 parataxis dilution,antibody
R3699 T5554 T5555 compound goat,anti-rabbit
R3700 T5555 T5557 compound anti-rabbit,IgG
R3701 T5556 T5555 punct -,anti-rabbit
R3702 T5557 T5553 dep IgG,dilution
R3703 T5558 T5557 acl conjugated,IgG
R3704 T5559 T5558 prep with,conjugated
R3705 T5560 T5559 pobj rhodamine,with
R3706 T5561 T5553 punct ", ",dilution
R3707 T5562 T5553 nummod 1,dilution
R3708 T5563 T5564 punct :,1000
R3709 T5564 T5562 prep 1000,1
R3710 T5565 T5553 punct ),dilution
R3711 T5566 T5548 prep for,incubated
R3712 T5567 T5568 nummod 30,min
R3713 T5568 T5566 pobj min,for
R3714 T5569 T5548 prep at,incubated
R3715 T5570 T5571 compound room,temperature
R3716 T5571 T5569 pobj temperature,at
R3717 T5572 T5541 punct ", ",washed
R3718 T5573 T5541 dep washed,washed
R3719 T5574 T5573 cc and,washed
R3720 T5575 T5576 advmod then,observed
R3721 T5576 T5573 conj observed,washed
R3722 T5577 T5576 prep under,observed
R3723 T5578 T5579 det an,microscope
R3724 T5579 T5577 pobj microscope,under
R3725 T5580 T5579 nmod Olympus,microscope
R3726 T5581 T5579 nmod BX,microscope
R3727 T5582 T5581 punct -,BX
R3728 T5583 T5581 nummod 5,BX
R3729 T5584 T5579 amod confocal,microscope
R3730 T5585 T5579 prep with,microscope
R3731 T5586 T5585 pobj Fluoview,with
R3732 T5587 T5541 punct .,washed
R3733 T5589 T5590 det The,sections
R3734 T5590 T5591 nsubjpass sections,fixed
R3735 T5592 T5590 acl stained,sections
R3736 T5593 T5592 prep at,stained
R3737 T5594 T5595 det the,Center
R3738 T5595 T5593 pobj Center,at
R3739 T5596 T5595 compound AMC,Center
R3740 T5597 T5595 compound Liver,Center
R3741 T5598 T5591 auxpass were,fixed
R3742 T5599 T5591 prep in,fixed
R3743 T5600 T5601 nummod 100,%
R3744 T5601 T5602 compound %,acetone
R3745 T5602 T5599 pobj acetone,in
R3746 T5603 T5591 prep for,fixed
R3747 T5604 T5605 nummod 30,min
R3748 T5605 T5603 pobj min,for
R3749 T5606 T5591 prep at,fixed
R3750 T5607 T5608 compound room,temperature
R3751 T5608 T5606 pobj temperature,at
R3752 T5609 T5591 punct ", ",fixed
R3753 T5610 T5591 dep blocked,fixed
R3754 T5611 T5610 prep in,blocked
R3755 T5612 T5613 det a,solution
R3756 T5613 T5611 pobj solution,in
R3757 T5614 T5613 prep of,solution
R3758 T5615 T5616 nmod PBS,%
R3759 T5616 T5614 pobj %,of
R3760 T5617 T5616 punct /,%
R3761 T5618 T5616 nummod 0.05,%
R3762 T5619 T5620 nmod Tween,%
R3763 T5620 T5616 appos %,%
R3764 T5621 T5619 punct -,Tween
R3765 T5622 T5619 nummod 20,Tween
R3766 T5623 T5620 punct /,%
R3767 T5624 T5620 nummod 5,%
R3768 T5625 T5626 compound goat,serum
R3769 T5626 T5616 appos serum,%
R3770 T5627 T5626 punct (,serum
R3771 T5628 T5626 appos GS,serum
R3772 T5629 T5626 punct ),serum
R3773 T5630 T5610 prep for,blocked
R3774 T5631 T5632 nummod one,hour
R3775 T5632 T5630 pobj hour,for
R3776 T5633 T5591 punct ", ",fixed
R3777 T5634 T5635 advmod then,incubated
R3778 T5635 T5591 dep incubated,fixed
R3779 T5636 T5635 prep with,incubated
R3780 T5637 T5636 pobj Anti-sterolin,with
R3781 T5638 T5637 punct -,Anti-sterolin
R3782 T5639 T5637 nummod 1,Anti-sterolin
R3783 T5640 T5641 punct (,dilution
R3784 T5641 T5637 parataxis dilution,Anti-sterolin
R3785 T5642 T5641 nummod 1,dilution
R3786 T5643 T5644 punct :,40
R3787 T5644 T5642 prep 40,1
R3788 T5645 T5641 punct ),dilution
R3789 T5646 T5637 cc and,Anti-sterolin
R3790 T5647 T5637 conj anti-Bsep,Anti-sterolin
R3791 T5648 T5649 punct (,dilution
R3792 T5649 T5647 parataxis dilution,anti-Bsep
R3793 T5650 T5649 nummod 1,dilution
R3794 T5651 T5652 punct :,200
R3795 T5652 T5650 prep 200,1
R3796 T5653 T5649 punct ),dilution
R3797 T5654 T5637 acl diluted,Anti-sterolin
R3798 T5655 T5654 prep in,diluted
R3799 T5656 T5657 compound PBS,GS
R3800 T5657 T5655 pobj GS,in
R3801 T5658 T5657 punct /,GS
R3802 T5659 T5657 compound Tween,GS
R3803 T5660 T5657 punct /,GS
R3804 T5661 T5654 punct ", ",diluted
R3805 T5662 T5654 prep for,diluted
R3806 T5663 T5664 nummod one,hour
R3807 T5664 T5662 pobj hour,for
R3808 T5665 T5591 punct .,fixed
R3809 T5667 T5668 advmod Thereafter,washed
R3810 T5669 T5668 punct ", ",washed
R3811 T5670 T5668 nsubjpass slides,washed
R3812 T5671 T5668 auxpass were,washed
R3813 T5672 T5673 nummod 3,min
R3814 T5673 T5668 npadvmod min,washed
R3815 T5674 T5672 punct ×,3
R3816 T5675 T5673 nummod 10,min
R3817 T5676 T5668 prep in,washed
R3818 T5677 T5678 compound PBS,Tween
R3819 T5678 T5676 pobj Tween,in
R3820 T5679 T5678 punct /,Tween
R3821 T5680 T5668 cc and,washed
R3822 T5681 T5682 det the,antibody
R3823 T5682 T5684 nsubjpass antibody,applied
R3824 T5683 T5682 amod secondary,antibody
R3825 T5684 T5668 conj applied,washed
R3826 T5685 T5682 punct (,antibody
R3827 T5686 T5687 compound goat,anti-rabbit
R3828 T5687 T5689 compound anti-rabbit,Alexa
R3829 T5688 T5687 punct -,anti-rabbit
R3830 T5689 T5682 appos Alexa,antibody
R3831 T5690 T5689 nummod 488,Alexa
R3832 T5691 T5684 punct ),applied
R3833 T5692 T5684 auxpass was,applied
R3834 T5693 T5684 prep in,applied
R3835 T5694 T5695 nummod 1,dilution
R3836 T5695 T5693 pobj dilution,in
R3837 T5696 T5697 punct :,1000
R3838 T5697 T5694 prep 1000,1
R3839 T5698 T5684 prep in,applied
R3840 T5699 T5700 compound PBST,GS
R3841 T5700 T5698 pobj GS,in
R3842 T5701 T5700 punct /,GS
R3843 T5702 T5684 prep for,applied
R3844 T5703 T5704 nummod one,hour
R3845 T5704 T5702 pobj hour,for
R3846 T5705 T5684 prep at,applied
R3847 T5706 T5707 compound room,temperature
R3848 T5707 T5705 pobj temperature,at
R3849 T5708 T5684 punct .,applied
R3850 T5710 T5711 prep After,mounted
R3851 T5712 T5710 pcomp washing,After
R3852 T5713 T5712 prep for,washing
R3853 T5714 T5715 nummod 3,min
R3854 T5715 T5713 pobj min,for
R3855 T5716 T5714 punct ×,3
R3856 T5717 T5715 nummod 10,min
R3857 T5718 T5719 det the,sections
R3858 T5719 T5711 nsubjpass sections,mounted
R3859 T5720 T5711 auxpass were,mounted
R3860 T5721 T5711 prep in,mounted
R3861 T5722 T5723 compound Dapi,Dapco
R3862 T5723 T5725 compound Dapco,solution
R3863 T5724 T5723 punct /,Dapco
R3864 T5725 T5721 pobj solution,in
R3865 T5726 T5711 punct .,mounted
R3866 T5728 T5729 compound Fluorescence,microscopy
R3867 T5729 T5730 nsubjpass microscopy,done
R3868 T5731 T5730 auxpass was,done
R3869 T5732 T5730 prep with,done
R3870 T5733 T5734 det a,microscope
R3871 T5734 T5732 pobj microscope,with
R3872 T5735 T5734 compound Leica,microscope
R3873 T5736 T5737 compound DM,RA2
R3874 T5737 T5734 compound RA2,microscope
R3875 T5738 T5734 acl equipped,microscope
R3876 T5739 T5738 prep with,equipped
R3877 T5740 T5741 det a,camera
R3878 T5741 T5739 pobj camera,with
R3879 T5742 T5741 compound Leica,camera
R3880 T5743 T5741 compound DC350F,camera
R3881 T5744 T5741 compound photo,camera
R3882 T5745 T5730 punct .,done
R3883 T5747 T5748 prep For,used
R3884 T5749 T5750 compound Dapi,staining
R3885 T5750 T5747 pobj staining,For
R3886 T5751 T5748 punct ", ",used
R3887 T5752 T5753 nummod 359,nm
R3888 T5753 T5748 nsubjpass nm,used
R3889 T5754 T5755 punct (,exposure
R3890 T5755 T5753 parataxis exposure,nm
R3891 T5756 T5757 nummod 30.38,ms
R3892 T5757 T5755 compound ms,exposure
R3893 T5758 T5755 punct ),exposure
R3894 T5759 T5748 auxpass was,used
R3895 T5760 T5748 cc and,used
R3896 T5761 T5762 prep for,used
R3897 T5762 T5748 conj used,used
R3898 T5763 T5764 nmod Abcg5,staining
R3899 T5764 T5761 pobj staining,for
R3900 T5765 T5763 cc and,Abcg5
R3901 T5766 T5763 conj Bsep,Abcg5
R3902 T5767 T5762 punct ", ",used
R3903 T5768 T5769 nummod 514,nm
R3904 T5769 T5762 nsubjpass nm,used
R3905 T5770 T5771 punct (,exposure
R3906 T5771 T5769 parataxis exposure,nm
R3907 T5772 T5771 prep for,exposure
R3908 T5773 T5772 pobj both,for
R3909 T5774 T5771 punct : ,exposure
R3910 T5775 T5776 nummod 1654.34,ms
R3911 T5776 T5771 compound ms,exposure
R3912 T5777 T5771 punct ),exposure
R3913 T5778 T5762 auxpass was,used
R3914 T5779 T5762 cc and,used
R3915 T5780 T5781 det the,pictures
R3916 T5781 T5782 nsubjpass pictures,analyzed
R3917 T5782 T5762 conj analyzed,used
R3918 T5783 T5782 auxpass were,analyzed
R3919 T5784 T5782 cc and,analyzed
R3920 T5785 T5782 conj overlaid,analyzed
R3921 T5786 T5785 advcl using,overlaid
R3922 T5787 T5788 compound FW4000,software
R3923 T5788 T5786 dobj software,using
R3924 T5789 T5790 punct (,Leica
R3925 T5790 T5788 parataxis Leica,software
R3926 T5791 T5790 punct ),Leica
R3927 T5792 T5782 punct .,analyzed