Id |
Subject |
Object |
Predicate |
Lexical cue |
T4469 |
0-9 |
VBG |
denotes |
Following |
T4471 |
10-11 |
DT |
denotes |
a |
T4473 |
12-16 |
CD |
denotes |
four |
T4475 |
16-17 |
HYPH |
denotes |
- |
T4474 |
17-21 |
NN |
denotes |
hour |
T4472 |
22-26 |
NN |
denotes |
fast |
T4476 |
27-29 |
IN |
denotes |
of |
T4477 |
30-32 |
CD |
denotes |
12 |
T4479 |
32-33 |
HYPH |
denotes |
- |
T4478 |
33-37 |
NN |
denotes |
week |
T4481 |
37-38 |
HYPH |
denotes |
- |
T4480 |
38-41 |
JJ |
denotes |
old |
T4482 |
42-46 |
NNS |
denotes |
mice |
T4483 |
47-50 |
VBN |
denotes |
fed |
T4484 |
51-53 |
IN |
denotes |
on |
T4485 |
54-61 |
JJ |
denotes |
regular |
T4487 |
62-68 |
NN |
denotes |
rodent |
T4488 |
69-73 |
NN |
denotes |
chow |
T4486 |
74-78 |
NN |
denotes |
diet |
T4489 |
78-79 |
: |
denotes |
; |
T4490 |
80-85 |
NN |
denotes |
blood |
T4491 |
86-89 |
CC |
denotes |
and |
T4492 |
90-97 |
NNS |
denotes |
tissues |
T4493 |
98-102 |
VBD |
denotes |
were |
T4470 |
103-112 |
VBN |
denotes |
collected |
T4494 |
113-118 |
IN |
denotes |
under |
T4495 |
119-130 |
NN |
denotes |
isofluorane |
T4496 |
131-141 |
NN |
denotes |
anesthesia |
T4497 |
142-146 |
IN |
denotes |
from |
T4498 |
147-151 |
DT |
denotes |
each |
T4499 |
152-154 |
IN |
denotes |
of |
T4500 |
155-158 |
DT |
denotes |
the |
T4502 |
159-164 |
NN |
denotes |
Abcg8 |
T4503 |
164-165 |
SYM |
denotes |
+ |
T4504 |
165-166 |
HYPH |
denotes |
/ |
T4505 |
166-167 |
SYM |
denotes |
+ |
T4506 |
167-169 |
, |
denotes |
, |
T4507 |
169-174 |
NN |
denotes |
Abcg8 |
T4508 |
174-175 |
SYM |
denotes |
+ |
T4509 |
175-176 |
HYPH |
denotes |
/ |
T4510 |
176-177 |
SYM |
denotes |
- |
T4511 |
178-181 |
CC |
denotes |
and |
T4512 |
182-187 |
NN |
denotes |
Abcg8 |
T4513 |
187-188 |
SYM |
denotes |
- |
T4514 |
188-189 |
HYPH |
denotes |
/ |
T4515 |
189-190 |
SYM |
denotes |
- |
T4501 |
191-195 |
NNS |
denotes |
mice |
T4516 |
196-197 |
-LRB- |
denotes |
( |
T4518 |
197-198 |
NN |
denotes |
n |
T4519 |
199-200 |
SYM |
denotes |
= |
T4517 |
201-202 |
CD |
denotes |
3 |
T4520 |
203-206 |
IN |
denotes |
for |
T4521 |
207-211 |
DT |
denotes |
each |
T4522 |
212-217 |
NN |
denotes |
group |
T4523 |
217-218 |
-RRB- |
denotes |
) |
T4524 |
218-219 |
. |
denotes |
. |
T4525 |
219-303 |
sentence |
denotes |
Blood was collected from the retro-orbital venous plexus by heparinized glass tube. |
T4526 |
220-225 |
NN |
denotes |
Blood |
T4528 |
226-229 |
VBD |
denotes |
was |
T4527 |
230-239 |
VBN |
denotes |
collected |
T4529 |
240-244 |
IN |
denotes |
from |
T4530 |
245-248 |
DT |
denotes |
the |
T4532 |
249-254 |
JJ |
denotes |
retro |
T4534 |
254-255 |
HYPH |
denotes |
- |
T4533 |
255-262 |
JJ |
denotes |
orbital |
T4535 |
263-269 |
NN |
denotes |
venous |
T4531 |
270-276 |
NN |
denotes |
plexus |
T4536 |
277-279 |
IN |
denotes |
by |
T4537 |
280-291 |
VBN |
denotes |
heparinized |
T4539 |
292-297 |
NN |
denotes |
glass |
T4538 |
298-302 |
NN |
denotes |
tube |
T4540 |
302-303 |
. |
denotes |
. |
T4541 |
303-378 |
sentence |
denotes |
The animals were sacrificed by cervical dislocation for tissue collection. |
T4542 |
304-307 |
DT |
denotes |
The |
T4543 |
308-315 |
NNS |
denotes |
animals |
T4545 |
316-320 |
VBD |
denotes |
were |
T4544 |
321-331 |
VBN |
denotes |
sacrificed |
T4546 |
332-334 |
IN |
denotes |
by |
T4547 |
335-343 |
JJ |
denotes |
cervical |
T4548 |
344-355 |
NN |
denotes |
dislocation |
T4549 |
356-359 |
IN |
denotes |
for |
T4550 |
360-366 |
NN |
denotes |
tissue |
T4551 |
367-377 |
NN |
denotes |
collection |
T4552 |
377-378 |
. |
denotes |
. |
T4553 |
378-606 |
sentence |
denotes |
Plasma (100 μl) was saponified in 1 N NaOH for one hour, 1.5 mL water added and sterols extracted with three sequential portions of 1.5 mL ethyl acetate, containing 70 μg 5α-cholestane as an internal standard, pooled and dried. |
T4554 |
379-385 |
NN |
denotes |
Plasma |
T4556 |
386-387 |
-LRB- |
denotes |
( |
T4558 |
387-390 |
CD |
denotes |
100 |
T4557 |
391-393 |
NN |
denotes |
μl |
T4559 |
393-394 |
-RRB- |
denotes |
) |
T4560 |
395-398 |
VBD |
denotes |
was |
T4555 |
399-409 |
VBN |
denotes |
saponified |
T4561 |
410-412 |
IN |
denotes |
in |
T4562 |
413-414 |
CD |
denotes |
1 |
T4563 |
415-416 |
NN |
denotes |
N |
T4564 |
417-421 |
NN |
denotes |
NaOH |
T4565 |
422-425 |
IN |
denotes |
for |
T4566 |
426-429 |
CD |
denotes |
one |
T4567 |
430-434 |
NN |
denotes |
hour |
T4568 |
434-436 |
, |
denotes |
, |
T4569 |
436-439 |
CD |
denotes |
1.5 |
T4570 |
440-442 |
NN |
denotes |
mL |
T4571 |
443-448 |
NN |
denotes |
water |
T4572 |
449-454 |
VBD |
denotes |
added |
T4573 |
455-458 |
CC |
denotes |
and |
T4574 |
459-466 |
NNS |
denotes |
sterols |
T4575 |
467-476 |
VBN |
denotes |
extracted |
T4576 |
477-481 |
IN |
denotes |
with |
T4577 |
482-487 |
CD |
denotes |
three |
T4579 |
488-498 |
JJ |
denotes |
sequential |
T4578 |
499-507 |
NNS |
denotes |
portions |
T4580 |
508-510 |
IN |
denotes |
of |
T4581 |
511-514 |
CD |
denotes |
1.5 |
T4582 |
515-517 |
NN |
denotes |
mL |
T4584 |
518-523 |
NN |
denotes |
ethyl |
T4583 |
524-531 |
NN |
denotes |
acetate |
T4585 |
531-533 |
, |
denotes |
, |
T4586 |
533-543 |
VBG |
denotes |
containing |
T4587 |
544-546 |
CD |
denotes |
70 |
T4588 |
547-549 |
NN |
denotes |
μg |
T4590 |
550-552 |
NN |
denotes |
5α |
T4591 |
552-553 |
HYPH |
denotes |
- |
T4589 |
553-563 |
NN |
denotes |
cholestane |
T4592 |
564-566 |
IN |
denotes |
as |
T4593 |
567-569 |
DT |
denotes |
an |
T4595 |
570-578 |
JJ |
denotes |
internal |
T4594 |
579-587 |
NN |
denotes |
standard |
T4596 |
587-589 |
, |
denotes |
, |
T4597 |
589-595 |
VBN |
denotes |
pooled |
T4598 |
596-599 |
CC |
denotes |
and |
T4599 |
600-605 |
VBN |
denotes |
dried |
T4600 |
605-606 |
. |
denotes |
. |
T4601 |
606-839 |
sentence |
denotes |
Mouse tissues (liver, spleen and brain) were weighed, homogenized in 1 mL phosphate buffer saline (PBS) with a dounce homogenizer (15 strokes at 500 rpm) and a small aliquot of the whole homogenate assayed for protein concentration. |
T4602 |
607-612 |
NN |
denotes |
Mouse |
T4603 |
613-620 |
NNS |
denotes |
tissues |
T4605 |
621-622 |
-LRB- |
denotes |
( |
T4606 |
622-627 |
NN |
denotes |
liver |
T4607 |
627-629 |
, |
denotes |
, |
T4608 |
629-635 |
NN |
denotes |
spleen |
T4609 |
636-639 |
CC |
denotes |
and |
T4610 |
640-645 |
NN |
denotes |
brain |
T4611 |
645-646 |
-RRB- |
denotes |
) |
T4612 |
647-651 |
VBD |
denotes |
were |
T4604 |
652-659 |
VBN |
denotes |
weighed |
T4613 |
659-661 |
, |
denotes |
, |
T4614 |
661-672 |
VBN |
denotes |
homogenized |
T4615 |
673-675 |
IN |
denotes |
in |
T4616 |
676-677 |
CD |
denotes |
1 |
T4617 |
678-680 |
NN |
denotes |
mL |
T4618 |
681-690 |
NN |
denotes |
phosphate |
T4619 |
691-697 |
NN |
denotes |
buffer |
T4620 |
698-704 |
NN |
denotes |
saline |
T4621 |
705-706 |
-LRB- |
denotes |
( |
T4622 |
706-709 |
NN |
denotes |
PBS |
T4623 |
709-710 |
-RRB- |
denotes |
) |
T4624 |
711-715 |
IN |
denotes |
with |
T4625 |
716-717 |
DT |
denotes |
a |
T4627 |
718-724 |
NN |
denotes |
dounce |
T4626 |
725-736 |
NN |
denotes |
homogenizer |
T4628 |
737-738 |
-LRB- |
denotes |
( |
T4630 |
738-740 |
CD |
denotes |
15 |
T4629 |
741-748 |
NNS |
denotes |
strokes |
T4631 |
749-751 |
IN |
denotes |
at |
T4632 |
752-755 |
CD |
denotes |
500 |
T4633 |
756-759 |
NN |
denotes |
rpm |
T4634 |
759-760 |
-RRB- |
denotes |
) |
T4635 |
761-764 |
CC |
denotes |
and |
T4636 |
765-766 |
DT |
denotes |
a |
T4638 |
767-772 |
JJ |
denotes |
small |
T4637 |
773-780 |
NN |
denotes |
aliquot |
T4639 |
781-783 |
IN |
denotes |
of |
T4640 |
784-787 |
DT |
denotes |
the |
T4642 |
788-793 |
JJ |
denotes |
whole |
T4641 |
794-804 |
NN |
denotes |
homogenate |
T4643 |
805-812 |
VBN |
denotes |
assayed |
T4644 |
813-816 |
IN |
denotes |
for |
T4645 |
817-824 |
NN |
denotes |
protein |
T4646 |
825-838 |
NN |
denotes |
concentration |
T4647 |
838-839 |
. |
denotes |
. |
T4648 |
839-933 |
sentence |
denotes |
Aliquots for sterol analysis from whole tissue homogenates were extracted as described above. |
T4649 |
840-848 |
NNS |
denotes |
Aliquots |
T4651 |
849-852 |
IN |
denotes |
for |
T4652 |
853-859 |
NN |
denotes |
sterol |
T4653 |
860-868 |
NN |
denotes |
analysis |
T4654 |
869-873 |
IN |
denotes |
from |
T4655 |
874-879 |
JJ |
denotes |
whole |
T4657 |
880-886 |
NN |
denotes |
tissue |
T4656 |
887-898 |
NNS |
denotes |
homogenates |
T4658 |
899-903 |
VBD |
denotes |
were |
T4650 |
904-913 |
VBN |
denotes |
extracted |
T4659 |
914-916 |
IN |
denotes |
as |
T4660 |
917-926 |
VBN |
denotes |
described |
T4661 |
927-932 |
RB |
denotes |
above |
T4662 |
932-933 |
. |
denotes |
. |
T4663 |
933-1268 |
sentence |
denotes |
Dried sterol samples were derivatized as trimethylsilylethers, redissolved in 10 μL hexane and 2 μL samples were injected into a capillary column (26 m length, 0.32 mm ID, 0.45 mm OD) coated with liquid CpWAX 57 CB (Chrompak, Bridgewater, NJ, USA) in a Hewlett-Packard Gas Chromatograph equipped with a flame ionization detector [28]. |
T4664 |
934-939 |
VBN |
denotes |
Dried |
T4666 |
940-946 |
NN |
denotes |
sterol |
T4665 |
947-954 |
NNS |
denotes |
samples |
T4668 |
955-959 |
VBD |
denotes |
were |
T4667 |
960-971 |
VBN |
denotes |
derivatized |
T4669 |
972-974 |
IN |
denotes |
as |
T4670 |
975-995 |
NNS |
denotes |
trimethylsilylethers |
T4671 |
995-997 |
, |
denotes |
, |
T4672 |
997-1008 |
VBN |
denotes |
redissolved |
T4673 |
1009-1011 |
IN |
denotes |
in |
T4674 |
1012-1014 |
CD |
denotes |
10 |
T4675 |
1015-1017 |
NN |
denotes |
μL |
T4676 |
1018-1024 |
NN |
denotes |
hexane |
T4677 |
1025-1028 |
CC |
denotes |
and |
T4678 |
1029-1030 |
CD |
denotes |
2 |
T4679 |
1031-1033 |
NN |
denotes |
μL |
T4680 |
1034-1041 |
NNS |
denotes |
samples |
T4682 |
1042-1046 |
VBD |
denotes |
were |
T4681 |
1047-1055 |
VBN |
denotes |
injected |
T4683 |
1056-1060 |
IN |
denotes |
into |
T4684 |
1061-1062 |
DT |
denotes |
a |
T4686 |
1063-1072 |
JJ |
denotes |
capillary |
T4685 |
1073-1079 |
NN |
denotes |
column |
T4687 |
1080-1081 |
-LRB- |
denotes |
( |
T4689 |
1081-1083 |
CD |
denotes |
26 |
T4690 |
1084-1085 |
NN |
denotes |
m |
T4688 |
1086-1092 |
NN |
denotes |
length |
T4691 |
1092-1094 |
, |
denotes |
, |
T4692 |
1094-1098 |
CD |
denotes |
0.32 |
T4693 |
1099-1101 |
NN |
denotes |
mm |
T4694 |
1102-1104 |
NN |
denotes |
ID |
T4695 |
1104-1106 |
, |
denotes |
, |
T4696 |
1106-1110 |
CD |
denotes |
0.45 |
T4697 |
1111-1113 |
NN |
denotes |
mm |
T4698 |
1114-1116 |
NN |
denotes |
OD |
T4699 |
1116-1117 |
-RRB- |
denotes |
) |
T4700 |
1118-1124 |
VBN |
denotes |
coated |
T4701 |
1125-1129 |
IN |
denotes |
with |
T4702 |
1130-1136 |
JJ |
denotes |
liquid |
T4704 |
1137-1142 |
NN |
denotes |
CpWAX |
T4705 |
1143-1145 |
CD |
denotes |
57 |
T4703 |
1146-1148 |
NN |
denotes |
CB |
T4706 |
1149-1150 |
-LRB- |
denotes |
( |
T4707 |
1150-1158 |
NNP |
denotes |
Chrompak |
T4708 |
1158-1160 |
, |
denotes |
, |
T4709 |
1160-1171 |
NNP |
denotes |
Bridgewater |
T4710 |
1171-1173 |
, |
denotes |
, |
T4711 |
1173-1175 |
NNP |
denotes |
NJ |
T4712 |
1175-1177 |
, |
denotes |
, |
T4713 |
1177-1180 |
NNP |
denotes |
USA |
T4714 |
1180-1181 |
-RRB- |
denotes |
) |
T4715 |
1182-1184 |
IN |
denotes |
in |
T4716 |
1185-1186 |
DT |
denotes |
a |
T4718 |
1187-1194 |
NNP |
denotes |
Hewlett |
T4720 |
1194-1195 |
HYPH |
denotes |
- |
T4719 |
1195-1202 |
NNP |
denotes |
Packard |
T4721 |
1203-1206 |
NNP |
denotes |
Gas |
T4717 |
1207-1220 |
NNP |
denotes |
Chromatograph |
T4722 |
1221-1229 |
VBN |
denotes |
equipped |
T4723 |
1230-1234 |
IN |
denotes |
with |
T4724 |
1235-1236 |
DT |
denotes |
a |
T4726 |
1237-1242 |
NN |
denotes |
flame |
T4727 |
1243-1253 |
NN |
denotes |
ionization |
T4725 |
1254-1262 |
NN |
denotes |
detector |
T4728 |
1263-1264 |
-LRB- |
denotes |
[ |
T4729 |
1264-1266 |
CD |
denotes |
28 |
T4730 |
1266-1267 |
-RRB- |
denotes |
] |
T4731 |
1267-1268 |
. |
denotes |
. |
T4732 |
1268-1377 |
sentence |
denotes |
Concentrations of plasma and tissue sterols were reported as mg/dL and μg/g wet weight tissue, respectively. |
T4733 |
1269-1283 |
NNS |
denotes |
Concentrations |
T4735 |
1284-1286 |
IN |
denotes |
of |
T4736 |
1287-1293 |
NN |
denotes |
plasma |
T4738 |
1294-1297 |
CC |
denotes |
and |
T4739 |
1298-1304 |
NN |
denotes |
tissue |
T4737 |
1305-1312 |
NNS |
denotes |
sterols |
T4740 |
1313-1317 |
VBD |
denotes |
were |
T4734 |
1318-1326 |
VBN |
denotes |
reported |
T4741 |
1327-1329 |
IN |
denotes |
as |
T4742 |
1330-1332 |
NN |
denotes |
mg |
T4744 |
1332-1333 |
SYM |
denotes |
/ |
T4745 |
1333-1335 |
NN |
denotes |
dL |
T4746 |
1336-1339 |
CC |
denotes |
and |
T4747 |
1340-1342 |
NN |
denotes |
μg |
T4748 |
1342-1343 |
SYM |
denotes |
/ |
T4749 |
1343-1344 |
NN |
denotes |
g |
T4750 |
1345-1348 |
JJ |
denotes |
wet |
T4751 |
1349-1355 |
NN |
denotes |
weight |
T4743 |
1356-1362 |
NN |
denotes |
tissue |
T4752 |
1362-1364 |
, |
denotes |
, |
T4753 |
1364-1376 |
RB |
denotes |
respectively |
T4754 |
1376-1377 |
. |
denotes |
. |
R2895 |
T4469 |
T4470 |
prep |
Following,collected |
R2896 |
T4471 |
T4472 |
det |
a,fast |
R2897 |
T4472 |
T4469 |
pobj |
fast,Following |
R2898 |
T4473 |
T4474 |
nummod |
four,hour |
R2899 |
T4474 |
T4472 |
compound |
hour,fast |
R2900 |
T4475 |
T4474 |
punct |
-,hour |
R2901 |
T4476 |
T4472 |
prep |
of,fast |
R2902 |
T4477 |
T4478 |
nummod |
12,week |
R2903 |
T4478 |
T4480 |
npadvmod |
week,old |
R2904 |
T4479 |
T4478 |
punct |
-,week |
R2905 |
T4480 |
T4482 |
amod |
old,mice |
R2906 |
T4481 |
T4480 |
punct |
-,old |
R2907 |
T4482 |
T4476 |
pobj |
mice,of |
R2908 |
T4483 |
T4482 |
acl |
fed,mice |
R2909 |
T4484 |
T4483 |
prep |
on,fed |
R2910 |
T4485 |
T4486 |
amod |
regular,diet |
R2911 |
T4486 |
T4484 |
pobj |
diet,on |
R2912 |
T4487 |
T4488 |
compound |
rodent,chow |
R2913 |
T4488 |
T4486 |
compound |
chow,diet |
R2914 |
T4489 |
T4470 |
punct |
;,collected |
R2915 |
T4490 |
T4470 |
nsubjpass |
blood,collected |
R2916 |
T4491 |
T4490 |
cc |
and,blood |
R2917 |
T4492 |
T4490 |
conj |
tissues,blood |
R2918 |
T4493 |
T4470 |
auxpass |
were,collected |
R2919 |
T4494 |
T4470 |
prep |
under,collected |
R2920 |
T4495 |
T4496 |
compound |
isofluorane,anesthesia |
R2921 |
T4496 |
T4494 |
pobj |
anesthesia,under |
R2922 |
T4497 |
T4470 |
prep |
from,collected |
R2923 |
T4498 |
T4497 |
pobj |
each,from |
R2924 |
T4499 |
T4498 |
prep |
of,each |
R2925 |
T4500 |
T4501 |
det |
the,mice |
R2926 |
T4501 |
T4499 |
pobj |
mice,of |
R2927 |
T4502 |
T4501 |
nmod |
Abcg8,mice |
R2928 |
T4503 |
T4502 |
punct |
+,Abcg8 |
R2929 |
T4504 |
T4502 |
punct |
/,Abcg8 |
R2930 |
T4505 |
T4502 |
punct |
+,Abcg8 |
R2931 |
T4506 |
T4502 |
punct |
", ",Abcg8 |
R2932 |
T4507 |
T4502 |
conj |
Abcg8,Abcg8 |
R2933 |
T4508 |
T4507 |
punct |
+,Abcg8 |
R2934 |
T4509 |
T4507 |
punct |
/,Abcg8 |
R2935 |
T4510 |
T4507 |
punct |
-,Abcg8 |
R2936 |
T4511 |
T4507 |
cc |
and,Abcg8 |
R2937 |
T4512 |
T4507 |
conj |
Abcg8,Abcg8 |
R2938 |
T4513 |
T4512 |
punct |
-,Abcg8 |
R2939 |
T4514 |
T4512 |
punct |
/,Abcg8 |
R2940 |
T4515 |
T4512 |
punct |
-,Abcg8 |
R2941 |
T4516 |
T4517 |
punct |
(,3 |
R2942 |
T4517 |
T4501 |
parataxis |
3,mice |
R2943 |
T4518 |
T4517 |
nsubj |
n,3 |
R2944 |
T4519 |
T4517 |
punct |
=,3 |
R2945 |
T4520 |
T4517 |
prep |
for,3 |
R2946 |
T4521 |
T4522 |
det |
each,group |
R2947 |
T4522 |
T4520 |
pobj |
group,for |
R2948 |
T4523 |
T4517 |
punct |
),3 |
R2949 |
T4524 |
T4470 |
punct |
.,collected |
R2950 |
T4526 |
T4527 |
nsubjpass |
Blood,collected |
R2951 |
T4528 |
T4527 |
auxpass |
was,collected |
R2952 |
T4529 |
T4527 |
prep |
from,collected |
R2953 |
T4530 |
T4531 |
det |
the,plexus |
R2954 |
T4531 |
T4529 |
pobj |
plexus,from |
R2955 |
T4532 |
T4533 |
amod |
retro,orbital |
R2956 |
T4533 |
T4531 |
amod |
orbital,plexus |
R2957 |
T4534 |
T4533 |
punct |
-,orbital |
R2958 |
T4535 |
T4531 |
compound |
venous,plexus |
R2959 |
T4536 |
T4527 |
prep |
by,collected |
R2960 |
T4537 |
T4538 |
amod |
heparinized,tube |
R2961 |
T4538 |
T4536 |
pobj |
tube,by |
R2962 |
T4539 |
T4538 |
compound |
glass,tube |
R2963 |
T4540 |
T4527 |
punct |
.,collected |
R2964 |
T4542 |
T4543 |
det |
The,animals |
R2965 |
T4543 |
T4544 |
nsubjpass |
animals,sacrificed |
R2966 |
T4545 |
T4544 |
auxpass |
were,sacrificed |
R2967 |
T4546 |
T4544 |
prep |
by,sacrificed |
R2968 |
T4547 |
T4548 |
amod |
cervical,dislocation |
R2969 |
T4548 |
T4546 |
pobj |
dislocation,by |
R2970 |
T4549 |
T4544 |
prep |
for,sacrificed |
R2971 |
T4550 |
T4551 |
compound |
tissue,collection |
R2972 |
T4551 |
T4549 |
pobj |
collection,for |
R2973 |
T4552 |
T4544 |
punct |
.,sacrificed |
R2974 |
T4554 |
T4555 |
nsubjpass |
Plasma,saponified |
R2975 |
T4556 |
T4557 |
punct |
(,μl |
R2976 |
T4557 |
T4554 |
parataxis |
μl,Plasma |
R2977 |
T4558 |
T4557 |
nummod |
100,μl |
R2978 |
T4559 |
T4557 |
punct |
),μl |
R2979 |
T4560 |
T4555 |
auxpass |
was,saponified |
R2980 |
T4561 |
T4555 |
prep |
in,saponified |
R2981 |
T4562 |
T4563 |
nummod |
1,N |
R2982 |
T4563 |
T4564 |
compound |
N,NaOH |
R2983 |
T4564 |
T4561 |
pobj |
NaOH,in |
R2984 |
T4565 |
T4555 |
prep |
for,saponified |
R2985 |
T4566 |
T4567 |
nummod |
one,hour |
R2986 |
T4567 |
T4565 |
pobj |
hour,for |
R2987 |
T4568 |
T4555 |
punct |
", ",saponified |
R2988 |
T4569 |
T4570 |
nummod |
1.5,mL |
R2989 |
T4570 |
T4571 |
compound |
mL,water |
R2990 |
T4571 |
T4572 |
nsubj |
water,added |
R2991 |
T4572 |
T4555 |
conj |
added,saponified |
R2992 |
T4573 |
T4572 |
cc |
and,added |
R2993 |
T4574 |
T4575 |
nsubj |
sterols,extracted |
R2994 |
T4575 |
T4572 |
conj |
extracted,added |
R2995 |
T4576 |
T4575 |
prep |
with,extracted |
R2996 |
T4577 |
T4578 |
nummod |
three,portions |
R2997 |
T4578 |
T4576 |
pobj |
portions,with |
R2998 |
T4579 |
T4578 |
amod |
sequential,portions |
R2999 |
T4580 |
T4578 |
prep |
of,portions |
R3000 |
T4581 |
T4582 |
nummod |
1.5,mL |
R3001 |
T4582 |
T4583 |
compound |
mL,acetate |
R3002 |
T4583 |
T4580 |
pobj |
acetate,of |
R3003 |
T4584 |
T4583 |
compound |
ethyl,acetate |
R3004 |
T4585 |
T4583 |
punct |
", ",acetate |
R3005 |
T4586 |
T4583 |
acl |
containing,acetate |
R3006 |
T4587 |
T4588 |
nummod |
70,μg |
R3007 |
T4588 |
T4589 |
compound |
μg,cholestane |
R3008 |
T4589 |
T4586 |
dobj |
cholestane,containing |
R3009 |
T4590 |
T4589 |
compound |
5α,cholestane |
R3010 |
T4591 |
T4589 |
punct |
-,cholestane |
R3011 |
T4592 |
T4589 |
prep |
as,cholestane |
R3012 |
T4593 |
T4594 |
det |
an,standard |
R3013 |
T4594 |
T4592 |
pobj |
standard,as |
R3014 |
T4595 |
T4594 |
amod |
internal,standard |
R3015 |
T4596 |
T4575 |
punct |
", ",extracted |
R3016 |
T4597 |
T4575 |
conj |
pooled,extracted |
R3017 |
T4598 |
T4597 |
cc |
and,pooled |
R3018 |
T4599 |
T4597 |
conj |
dried,pooled |
R3019 |
T4600 |
T4575 |
punct |
.,extracted |
R3020 |
T4602 |
T4603 |
compound |
Mouse,tissues |
R3021 |
T4603 |
T4604 |
nsubjpass |
tissues,weighed |
R3022 |
T4605 |
T4603 |
punct |
(,tissues |
R3023 |
T4606 |
T4603 |
appos |
liver,tissues |
R3024 |
T4607 |
T4606 |
punct |
", ",liver |
R3025 |
T4608 |
T4606 |
conj |
spleen,liver |
R3026 |
T4609 |
T4608 |
cc |
and,spleen |
R3027 |
T4610 |
T4608 |
conj |
brain,spleen |
R3028 |
T4611 |
T4604 |
punct |
),weighed |
R3029 |
T4612 |
T4604 |
auxpass |
were,weighed |
R3030 |
T4613 |
T4604 |
punct |
", ",weighed |
R3031 |
T4614 |
T4604 |
dep |
homogenized,weighed |
R3032 |
T4615 |
T4614 |
prep |
in,homogenized |
R3033 |
T4616 |
T4617 |
nummod |
1,mL |
R3034 |
T4617 |
T4615 |
pobj |
mL,in |
R3035 |
T4618 |
T4619 |
compound |
phosphate,buffer |
R3036 |
T4619 |
T4620 |
compound |
buffer,saline |
R3037 |
T4620 |
T4617 |
appos |
saline,mL |
R3038 |
T4621 |
T4620 |
punct |
(,saline |
R3039 |
T4622 |
T4620 |
appos |
PBS,saline |
R3040 |
T4623 |
T4617 |
punct |
),mL |
R3041 |
T4624 |
T4617 |
prep |
with,mL |
R3042 |
T4625 |
T4626 |
det |
a,homogenizer |
R3043 |
T4626 |
T4624 |
pobj |
homogenizer,with |
R3044 |
T4627 |
T4626 |
compound |
dounce,homogenizer |
R3045 |
T4628 |
T4629 |
punct |
(,strokes |
R3046 |
T4629 |
T4626 |
parataxis |
strokes,homogenizer |
R3047 |
T4630 |
T4629 |
nummod |
15,strokes |
R3048 |
T4631 |
T4629 |
prep |
at,strokes |
R3049 |
T4632 |
T4633 |
nummod |
500,rpm |
R3050 |
T4633 |
T4631 |
pobj |
rpm,at |
R3051 |
T4634 |
T4629 |
punct |
),strokes |
R3052 |
T4635 |
T4626 |
cc |
and,homogenizer |
R3053 |
T4636 |
T4637 |
det |
a,aliquot |
R3054 |
T4637 |
T4626 |
conj |
aliquot,homogenizer |
R3055 |
T4638 |
T4637 |
amod |
small,aliquot |
R3056 |
T4639 |
T4637 |
prep |
of,aliquot |
R3057 |
T4640 |
T4641 |
det |
the,homogenate |
R3058 |
T4641 |
T4639 |
pobj |
homogenate,of |
R3059 |
T4642 |
T4641 |
amod |
whole,homogenate |
R3060 |
T4643 |
T4641 |
acl |
assayed,homogenate |
R3061 |
T4644 |
T4643 |
prep |
for,assayed |
R3062 |
T4645 |
T4646 |
compound |
protein,concentration |
R3063 |
T4646 |
T4644 |
pobj |
concentration,for |
R3064 |
T4647 |
T4604 |
punct |
.,weighed |
R3065 |
T4649 |
T4650 |
nsubjpass |
Aliquots,extracted |
R3066 |
T4651 |
T4649 |
prep |
for,Aliquots |
R3067 |
T4652 |
T4653 |
compound |
sterol,analysis |
R3068 |
T4653 |
T4651 |
pobj |
analysis,for |
R3069 |
T4654 |
T4649 |
prep |
from,Aliquots |
R3070 |
T4655 |
T4656 |
amod |
whole,homogenates |
R3071 |
T4656 |
T4654 |
pobj |
homogenates,from |
R3072 |
T4657 |
T4656 |
compound |
tissue,homogenates |
R3073 |
T4658 |
T4650 |
auxpass |
were,extracted |
R3074 |
T4659 |
T4660 |
mark |
as,described |
R3075 |
T4660 |
T4650 |
advcl |
described,extracted |
R3076 |
T4661 |
T4660 |
advmod |
above,described |
R3077 |
T4662 |
T4650 |
punct |
.,extracted |
R3078 |
T4664 |
T4665 |
amod |
Dried,samples |
R3079 |
T4665 |
T4667 |
nsubjpass |
samples,derivatized |
R3080 |
T4666 |
T4665 |
compound |
sterol,samples |
R3081 |
T4668 |
T4667 |
auxpass |
were,derivatized |
R3082 |
T4669 |
T4667 |
prep |
as,derivatized |
R3083 |
T4670 |
T4669 |
pobj |
trimethylsilylethers,as |
R3084 |
T4671 |
T4667 |
punct |
", ",derivatized |
R3085 |
T4672 |
T4667 |
dep |
redissolved,derivatized |
R3086 |
T4673 |
T4672 |
prep |
in,redissolved |
R3087 |
T4674 |
T4675 |
nummod |
10,μL |
R3088 |
T4675 |
T4676 |
compound |
μL,hexane |
R3089 |
T4676 |
T4673 |
pobj |
hexane,in |
R3090 |
T4677 |
T4667 |
cc |
and,derivatized |
R3091 |
T4678 |
T4679 |
nummod |
2,μL |
R3092 |
T4679 |
T4680 |
compound |
μL,samples |
R3093 |
T4680 |
T4681 |
nsubjpass |
samples,injected |
R3094 |
T4681 |
T4667 |
conj |
injected,derivatized |
R3095 |
T4682 |
T4681 |
auxpass |
were,injected |
R3096 |
T4683 |
T4681 |
prep |
into,injected |
R3097 |
T4684 |
T4685 |
det |
a,column |
R3098 |
T4685 |
T4683 |
pobj |
column,into |
R3099 |
T4686 |
T4685 |
amod |
capillary,column |
R3100 |
T4687 |
T4688 |
punct |
(,length |
R3101 |
T4688 |
T4685 |
parataxis |
length,column |
R3102 |
T4689 |
T4690 |
nummod |
26,m |
R3103 |
T4690 |
T4688 |
compound |
m,length |
R3104 |
T4691 |
T4688 |
punct |
", ",length |
R3105 |
T4692 |
T4693 |
nummod |
0.32,mm |
R3106 |
T4693 |
T4694 |
compound |
mm,ID |
R3107 |
T4694 |
T4688 |
appos |
ID,length |
R3108 |
T4695 |
T4688 |
punct |
", ",length |
R3109 |
T4696 |
T4697 |
nummod |
0.45,mm |
R3110 |
T4697 |
T4698 |
compound |
mm,OD |
R3111 |
T4698 |
T4688 |
appos |
OD,length |
R3112 |
T4699 |
T4688 |
punct |
),length |
R3113 |
T4700 |
T4685 |
acl |
coated,column |
R3114 |
T4701 |
T4700 |
prep |
with,coated |
R3115 |
T4702 |
T4703 |
amod |
liquid,CB |
R3116 |
T4703 |
T4701 |
pobj |
CB,with |
R3117 |
T4704 |
T4703 |
nmod |
CpWAX,CB |
R3118 |
T4705 |
T4703 |
nummod |
57,CB |
R3119 |
T4706 |
T4707 |
punct |
(,Chrompak |
R3120 |
T4707 |
T4703 |
parataxis |
Chrompak,CB |
R3121 |
T4708 |
T4707 |
punct |
", ",Chrompak |
R3122 |
T4709 |
T4707 |
npadvmod |
Bridgewater,Chrompak |
R3123 |
T4710 |
T4707 |
punct |
", ",Chrompak |
R3124 |
T4711 |
T4707 |
npadvmod |
NJ,Chrompak |
R3125 |
T4712 |
T4707 |
punct |
", ",Chrompak |
R3126 |
T4713 |
T4707 |
npadvmod |
USA,Chrompak |
R3127 |
T4714 |
T4707 |
punct |
),Chrompak |
R3128 |
T4715 |
T4685 |
prep |
in,column |
R3129 |
T4716 |
T4717 |
det |
a,Chromatograph |
R3130 |
T4717 |
T4715 |
pobj |
Chromatograph,in |
R3131 |
T4718 |
T4719 |
compound |
Hewlett,Packard |
R3132 |
T4719 |
T4717 |
compound |
Packard,Chromatograph |
R3133 |
T4720 |
T4719 |
punct |
-,Packard |
R3134 |
T4721 |
T4717 |
compound |
Gas,Chromatograph |
R3135 |
T4722 |
T4717 |
acl |
equipped,Chromatograph |
R3136 |
T4723 |
T4722 |
prep |
with,equipped |
R3137 |
T4724 |
T4725 |
det |
a,detector |
R3138 |
T4725 |
T4723 |
pobj |
detector,with |
R3139 |
T4726 |
T4727 |
compound |
flame,ionization |
R3140 |
T4727 |
T4725 |
compound |
ionization,detector |
R3141 |
T4728 |
T4729 |
punct |
[,28 |
R3142 |
T4729 |
T4681 |
parataxis |
28,injected |
R3143 |
T4730 |
T4729 |
punct |
],28 |
R3144 |
T4731 |
T4681 |
punct |
.,injected |
R3145 |
T4733 |
T4734 |
nsubjpass |
Concentrations,reported |
R3146 |
T4735 |
T4733 |
prep |
of,Concentrations |
R3147 |
T4736 |
T4737 |
nmod |
plasma,sterols |
R3148 |
T4737 |
T4735 |
pobj |
sterols,of |
R3149 |
T4738 |
T4736 |
cc |
and,plasma |
R3150 |
T4739 |
T4736 |
conj |
tissue,plasma |
R3151 |
T4740 |
T4734 |
auxpass |
were,reported |
R3152 |
T4741 |
T4734 |
prep |
as,reported |
R3153 |
T4742 |
T4743 |
nmod |
mg,tissue |
R3154 |
T4743 |
T4741 |
pobj |
tissue,as |
R3155 |
T4744 |
T4745 |
punct |
/,dL |
R3156 |
T4745 |
T4742 |
prep |
dL,mg |
R3157 |
T4746 |
T4742 |
cc |
and,mg |
R3158 |
T4747 |
T4742 |
conj |
μg,mg |
R3159 |
T4748 |
T4749 |
punct |
/,g |
R3160 |
T4749 |
T4747 |
prep |
g,μg |
R3161 |
T4750 |
T4751 |
amod |
wet,weight |
R3162 |
T4751 |
T4743 |
compound |
weight,tissue |
R3163 |
T4752 |
T4734 |
punct |
", ",reported |
R3164 |
T4753 |
T4734 |
advmod |
respectively,reported |
R3165 |
T4754 |
T4734 |
punct |
.,reported |