PMC:341451 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T240	0-4	JJ	denotes	Late
T242	0-83	sentence	denotes	Late gestational lung hypoplasia in a mouse model of the Smith-Lemli-Opitz syndrome
T241	5-16	JJ	denotes	gestational
T244	17-21	NN	denotes	lung
T243	22-32	NN	denotes	hypoplasia
T245	33-35	IN	denotes	in
T246	36-37	DT	denotes	a
T248	38-43	NN	denotes	mouse
T247	44-49	NN	denotes	model
T249	50-52	IN	denotes	of
T250	53-56	DT	denotes	the
T252	57-62	NNP	denotes	Smith
T254	62-63	HYPH	denotes	-
T255	63-68	NNP	denotes	Lemli
T256	68-69	HYPH	denotes	-
T253	69-74	NNP	denotes	Opitz
T251	75-83	NN	denotes	syndrome
T257	83-84	sentence	denotes
T258	94-104	sentence	denotes	Background
T259	94-104	NN	denotes	Background
T260	104-200	sentence	denotes	Normal post-squalene cholesterol biosynthesis is important for mammalian embryonic development.
T261	105-111	JJ	denotes	Normal
T263	112-125	JJ	denotes	post-squalene
T264	126-137	NN	denotes	cholesterol
T262	138-150	NN	denotes	biosynthesis
T265	151-153	VBZ	denotes	is
T266	154-163	JJ	denotes	important
T267	164-167	IN	denotes	for
T268	168-177	JJ	denotes	mammalian
T270	178-187	JJ	denotes	embryonic
T269	188-199	NN	denotes	development
T271	199-200	.	denotes	.
T272	200-365	sentence	denotes	Neonatal mice lacking functional dehydrocholesterol Δ7-reductase (Dhcr7), a model for the human disease of Smith-Lemli-Opitz syndrome, die within 24 hours of birth.
T273	201-209	JJ	denotes	Neonatal
T274	210-214	NNS	denotes	mice
T276	215-222	VBG	denotes	lacking
T277	223-233	JJ	denotes	functional
T279	234-252	NN	denotes	dehydrocholesterol
T280	253-255	NN	denotes	Δ7
T281	255-256	HYPH	denotes	-
T278	256-265	NN	denotes	reductase
T282	266-267	-LRB-	denotes	(
T283	267-272	NN	denotes	Dhcr7
T284	272-273	-RRB-	denotes	)
T285	273-275	,	denotes	,
T286	275-276	DT	denotes	a
T287	277-282	NN	denotes	model
T288	283-286	IN	denotes	for
T289	287-290	DT	denotes	the
T291	291-296	JJ	denotes	human
T290	297-304	NN	denotes	disease
T292	305-307	IN	denotes	of
T293	308-313	NNP	denotes	Smith
T295	313-314	HYPH	denotes	-
T296	314-319	NNP	denotes	Lemli
T297	319-320	HYPH	denotes	-
T294	320-325	NNP	denotes	Opitz
T298	326-334	NN	denotes	syndrome
T299	334-336	,	denotes	,
T275	336-339	VBP	denotes	die
T300	340-346	IN	denotes	within
T301	347-349	CD	denotes	24
T302	350-355	NNS	denotes	hours
T303	356-358	IN	denotes	of
T304	359-364	NN	denotes	birth
T305	364-365	.	denotes	.
T306	365-540	sentence	denotes	Although they have a number of biochemical and structural abnormalities, one cause of death is from apparent respiratory failure due to developmental pulmonary abnormalities.
T307	366-374	IN	denotes	Although
T309	375-379	PRP	denotes	they
T308	380-384	VBP	denotes	have
T311	385-386	DT	denotes	a
T312	387-393	NN	denotes	number
T313	394-396	IN	denotes	of
T314	397-408	JJ	denotes	biochemical
T316	409-412	CC	denotes	and
T317	413-423	JJ	denotes	structural
T315	424-437	NNS	denotes	abnormalities
T318	437-439	,	denotes	,
T319	439-442	CD	denotes	one
T320	443-448	NN	denotes	cause
T321	449-451	IN	denotes	of
T322	452-457	NN	denotes	death
T310	458-460	VBZ	denotes	is
T323	461-465	IN	denotes	from
T324	466-474	JJ	denotes	apparent
T326	475-486	JJ	denotes	respiratory
T325	487-494	NN	denotes	failure
T327	495-498	IN	denotes	due
T328	499-501	IN	denotes	to
T329	502-515	JJ	denotes	developmental
T331	516-525	JJ	denotes	pulmonary
T330	526-539	NNS	denotes	abnormalities
T332	539-540	.	denotes	.
T333	540-549	sentence	denotes	Results
T334	542-549	NNS	denotes	Results
T335	549-659	sentence	denotes	In this study, we characterized further the role of cholesterol deficiency in lung development of these mice.
T336	550-552	IN	denotes	In
T338	553-557	DT	denotes	this
T339	558-563	NN	denotes	study
T340	563-565	,	denotes	,
T341	565-567	PRP	denotes	we
T337	568-581	VBD	denotes	characterized
T342	582-589	RB	denotes	further
T343	590-593	DT	denotes	the
T344	594-598	NN	denotes	role
T345	599-601	IN	denotes	of
T346	602-613	NN	denotes	cholesterol
T347	614-624	NN	denotes	deficiency
T348	625-627	IN	denotes	in
T349	628-632	NN	denotes	lung
T350	633-644	NN	denotes	development
T351	645-647	IN	denotes	of
T352	648-653	DT	denotes	these
T353	654-658	NNS	denotes	mice
T354	658-659	.	denotes	.
T355	659-751	sentence	denotes	Significant growth retardation, beginning at E14.5~E16.5, was observed in Dhcr7-/- embryos.
T356	660-671	JJ	denotes	Significant
T358	672-678	NN	denotes	growth
T357	679-690	NN	denotes	retardation
T360	690-692	,	denotes	,
T361	692-701	VBG	denotes	beginning
T362	702-704	IN	denotes	at
T363	705-710	NN	denotes	E14.5
T364	710-711	SYM	denotes	~
T365	711-716	NN	denotes	E16.5
T366	716-718	,	denotes	,
T367	718-721	VBD	denotes	was
T359	722-730	VBN	denotes	observed
T368	731-733	IN	denotes	in
T369	734-739	NN	denotes	Dhcr7
T371	739-740	SYM	denotes	-
T372	740-741	SYM	denotes	/
T373	741-742	SYM	denotes	-
T370	743-750	NNS	denotes	embryos
T374	750-751	.	denotes	.
T375	751-895	sentence	denotes	Normal lobation but smaller lungs with a significant decrease in lung-to-body weight ratio was noted in Dhcr7-/- embryos, compared to controls.
T376	752-758	JJ	denotes	Normal
T377	759-767	NN	denotes	lobation
T379	768-771	CC	denotes	but
T380	772-779	JJR	denotes	smaller
T381	780-785	NNS	denotes	lungs
T382	786-790	IN	denotes	with
T383	791-792	DT	denotes	a
T385	793-804	JJ	denotes	significant
T384	805-813	NN	denotes	decrease
T386	814-816	IN	denotes	in
T387	817-821	NN	denotes	lung
T389	821-822	HYPH	denotes	-
T390	822-824	IN	denotes	to
T391	824-825	HYPH	denotes	-
T392	825-829	NN	denotes	body
T393	830-836	NN	denotes	weight
T388	837-842	NN	denotes	ratio
T394	843-846	VBD	denotes	was
T378	847-852	VBN	denotes	noted
T395	853-855	IN	denotes	in
T396	856-861	NN	denotes	Dhcr7
T398	861-862	SYM	denotes	-
T399	862-863	SYM	denotes	/
T400	863-864	SYM	denotes	-
T397	865-872	NNS	denotes	embryos
T401	872-874	,	denotes	,
T402	874-882	VBN	denotes	compared
T403	883-885	IN	denotes	to
T404	886-894	NNS	denotes	controls
T405	894-895	.	denotes	.
T406	895-1075	sentence	denotes	Lung branching morphogenesis was comparable between Dhcr7-/- and controls at early stages, but delayed saccular development was visible in all Dhcr7-/- embryos from E17.5 onwards.
T407	896-900	NN	denotes	Lung
T408	901-910	NN	denotes	branching
T409	911-924	NN	denotes	morphogenesis
T410	925-928	VBD	denotes	was
T411	929-939	JJ	denotes	comparable
T412	940-947	IN	denotes	between
T413	948-953	NN	denotes	Dhcr7
T414	953-954	SYM	denotes	-
T415	954-955	SYM	denotes	/
T416	955-956	SYM	denotes	-
T417	957-960	CC	denotes	and
T418	961-969	NNS	denotes	controls
T419	970-972	IN	denotes	at
T420	973-978	JJ	denotes	early
T421	979-985	NNS	denotes	stages
T422	985-987	,	denotes	,
T423	987-990	CC	denotes	but
T424	991-998	VBN	denotes	delayed
T426	999-1007	JJ	denotes	saccular
T425	1008-1019	NN	denotes	development
T427	1020-1023	VBD	denotes	was
T428	1024-1031	JJ	denotes	visible
T429	1032-1034	IN	denotes	in
T430	1035-1038	DT	denotes	all
T432	1039-1044	NN	denotes	Dhcr7
T433	1044-1045	SYM	denotes	-
T434	1045-1046	SYM	denotes	/
T435	1046-1047	SYM	denotes	-
T431	1048-1055	NNS	denotes	embryos
T436	1056-1060	IN	denotes	from
T438	1061-1066	NN	denotes	E17.5
T437	1067-1074	RB	denotes	onwards
T439	1074-1075	.	denotes	.
T440	1075-1296	sentence	denotes	Impaired pre-alveolar development of varying severity, inhibited cell proliferation, delayed differentiation of type I alveolar epithelial cells (AECs) and delayed vascular development were all evident in knockout lungs.
T441	1076-1084	VBN	denotes	Impaired
T443	1085-1097	JJ	denotes	pre-alveolar
T442	1098-1109	NN	denotes	development
T445	1110-1112	IN	denotes	of
T446	1113-1120	VBG	denotes	varying
T447	1121-1129	NN	denotes	severity
T448	1129-1131	,	denotes	,
T449	1131-1140	VBN	denotes	inhibited
T451	1141-1145	NN	denotes	cell
T450	1146-1159	NN	denotes	proliferation
T452	1159-1161	,	denotes	,
T453	1161-1168	VBN	denotes	delayed
T454	1169-1184	NN	denotes	differentiation
T455	1185-1187	IN	denotes	of
T456	1188-1192	NN	denotes	type
T457	1193-1194	CD	denotes	I
T458	1195-1203	JJ	denotes	alveolar
T460	1204-1214	JJ	denotes	epithelial
T459	1215-1220	NNS	denotes	cells
T461	1221-1222	-LRB-	denotes	(
T462	1222-1226	NNS	denotes	AECs
T463	1226-1227	-RRB-	denotes	)
T464	1228-1231	CC	denotes	and
T465	1232-1239	VBN	denotes	delayed
T467	1240-1248	JJ	denotes	vascular
T466	1249-1260	NN	denotes	development
T444	1261-1265	VBD	denotes	were
T468	1266-1269	RB	denotes	all
T469	1270-1277	JJ	denotes	evident
T470	1278-1280	IN	denotes	in
T471	1281-1289	NN	denotes	knockout
T472	1290-1295	NNS	denotes	lungs
T473	1295-1296	.	denotes	.
T474	1296-1418	sentence	denotes	Differentiation of type II AECs was apparently normal as judged by surfactant protein (SP) mRNAs and SP-C immunostaining.
T475	1297-1312	NN	denotes	Differentiation
T477	1313-1315	IN	denotes	of
T478	1316-1320	NN	denotes	type
T480	1321-1323	CD	denotes	II
T479	1324-1328	NNS	denotes	AECs
T476	1329-1332	VBD	denotes	was
T481	1333-1343	RB	denotes	apparently
T482	1344-1350	JJ	denotes	normal
T483	1351-1353	IN	denotes	as
T484	1354-1360	VBN	denotes	judged
T485	1361-1363	IN	denotes	by
T486	1364-1374	NN	denotes	surfactant
T487	1375-1382	NN	denotes	protein
T489	1383-1384	-LRB-	denotes	(
T490	1384-1386	NN	denotes	SP
T491	1386-1387	-RRB-	denotes	)
T488	1388-1393	NNS	denotes	mRNAs
T492	1394-1397	CC	denotes	and
T493	1398-1400	NN	denotes	SP
T495	1400-1401	HYPH	denotes	-
T494	1401-1402	NN	denotes	C
T496	1403-1417	NN	denotes	immunostaining
T497	1417-1418	.	denotes	.
T498	1418-1539	sentence	denotes	A significant amount of cholesterol was detectable in knockout lungs, implicating some maternal transfer of cholesterol.
T499	1419-1420	DT	denotes	A
T501	1421-1432	JJ	denotes	significant
T500	1433-1439	NN	denotes	amount
T503	1440-1442	IN	denotes	of
T504	1443-1454	NN	denotes	cholesterol
T502	1455-1458	VBD	denotes	was
T505	1459-1469	JJ	denotes	detectable
T506	1470-1472	IN	denotes	in
T507	1473-1481	NN	denotes	knockout
T508	1482-1487	NNS	denotes	lungs
T509	1487-1489	,	denotes	,
T510	1489-1500	VBG	denotes	implicating
T511	1501-1505	DT	denotes	some
T513	1506-1514	JJ	denotes	maternal
T512	1515-1523	NN	denotes	transfer
T514	1524-1526	IN	denotes	of
T515	1527-1538	NN	denotes	cholesterol
T516	1538-1539	.	denotes	.
T517	1539-1803	sentence	denotes	No significant differences of the spatial-temporal localization of sonic hedgehog (Shh) or its downstream targets by immunohistochemistry were detected between knockout and wild-type lungs and Shh autoprocessing occurred normally in tissues from Dhcr7-/- embryos.
T518	1540-1542	DT	denotes	No
T520	1543-1554	JJ	denotes	significant
T519	1555-1566	NNS	denotes	differences
T522	1567-1569	IN	denotes	of
T523	1570-1573	DT	denotes	the
T525	1574-1581	JJ	denotes	spatial
T527	1581-1582	HYPH	denotes	-
T526	1582-1590	JJ	denotes	temporal
T524	1591-1603	NN	denotes	localization
T528	1604-1606	IN	denotes	of
T529	1607-1612	JJ	denotes	sonic
T530	1613-1621	NN	denotes	hedgehog
T531	1622-1623	-LRB-	denotes	(
T532	1623-1626	NN	denotes	Shh
T533	1626-1627	-RRB-	denotes	)
T534	1628-1630	CC	denotes	or
T535	1631-1634	PRP$	denotes	its
T537	1635-1645	JJ	denotes	downstream
T536	1646-1653	NNS	denotes	targets
T538	1654-1656	IN	denotes	by
T539	1657-1677	NN	denotes	immunohistochemistry
T540	1678-1682	VBD	denotes	were
T521	1683-1691	VBN	denotes	detected
T541	1692-1699	IN	denotes	between
T542	1700-1708	NN	denotes	knockout
T544	1709-1712	CC	denotes	and
T545	1713-1717	JJ	denotes	wild
T547	1717-1718	HYPH	denotes	-
T546	1718-1722	NN	denotes	type
T543	1723-1728	NNS	denotes	lungs
T548	1729-1732	CC	denotes	and
T549	1733-1736	NN	denotes	Shh
T550	1737-1751	NN	denotes	autoprocessing
T551	1752-1760	VBD	denotes	occurred
T552	1761-1769	RB	denotes	normally
T553	1770-1772	IN	denotes	in
T554	1773-1780	NNS	denotes	tissues
T555	1781-1785	IN	denotes	from
T556	1786-1791	NN	denotes	Dhcr7
T558	1791-1792	SYM	denotes	-
T559	1792-1793	SYM	denotes	/
T560	1793-1794	SYM	denotes	-
T557	1795-1802	NNS	denotes	embryos
T561	1802-1803	.	denotes	.
T562	1803-1815	sentence	denotes	Conclusion
T563	1805-1815	NN	denotes	Conclusion
T564	1815-2110	sentence	denotes	Our data indicated that cholesterol deficiency caused by Dhcr7 null was associated with a distinct lung saccular hypoplasia, characterized by failure to terminally differentiate alveolar sacs, a delayed differentiation of type I AECs and an immature vascular network at late gestational stages.
T565	1816-1819	PRP$	denotes	Our
T566	1820-1824	NNS	denotes	data
T567	1825-1834	VBD	denotes	indicated
T568	1835-1839	IN	denotes	that
T570	1840-1851	NN	denotes	cholesterol
T571	1852-1862	NN	denotes	deficiency
T572	1863-1869	VBN	denotes	caused
T573	1870-1872	IN	denotes	by
T574	1873-1878	NN	denotes	Dhcr7
T575	1879-1883	NN	denotes	null
T576	1884-1887	VBD	denotes	was
T569	1888-1898	VBN	denotes	associated
T577	1899-1903	IN	denotes	with
T578	1904-1905	DT	denotes	a
T580	1906-1914	JJ	denotes	distinct
T581	1915-1919	NN	denotes	lung
T582	1920-1928	JJ	denotes	saccular
T579	1929-1939	NN	denotes	hypoplasia
T583	1939-1941	,	denotes	,
T584	1941-1954	VBN	denotes	characterized
T585	1955-1957	IN	denotes	by
T586	1958-1965	NN	denotes	failure
T587	1966-1968	TO	denotes	to
T589	1969-1979	RB	denotes	terminally
T588	1980-1993	VB	denotes	differentiate
T590	1994-2002	JJ	denotes	alveolar
T591	2003-2007	NNS	denotes	sacs
T592	2007-2009	,	denotes	,
T593	2009-2010	DT	denotes	a
T595	2011-2018	VBN	denotes	delayed
T594	2019-2034	NN	denotes	differentiation
T596	2035-2037	IN	denotes	of
T597	2038-2042	NN	denotes	type
T599	2043-2044	CD	denotes	I
T598	2045-2049	NNS	denotes	AECs
T600	2050-2053	CC	denotes	and
T601	2054-2056	DT	denotes	an
T603	2057-2065	JJ	denotes	immature
T604	2066-2074	JJ	denotes	vascular
T602	2075-2082	NN	denotes	network
T605	2083-2085	IN	denotes	at
T606	2086-2090	JJ	denotes	late
T608	2091-2102	JJ	denotes	gestational
T607	2103-2109	NNS	denotes	stages
T609	2109-2110	.	denotes	.
T610	2110-2342	sentence	denotes	The molecular mechanism of impaired lung development associated with sterol deficiency by Dhcr7 loss is still unknown, but these results do not support the involvement of dysregulated Shh-Patched-Gli pathway in causing this defect.
T611	2111-2114	DT	denotes	The
T613	2115-2124	JJ	denotes	molecular
T612	2125-2134	NN	denotes	mechanism
T615	2135-2137	IN	denotes	of
T616	2138-2146	JJ	denotes	impaired
T618	2147-2151	NN	denotes	lung
T617	2152-2163	NN	denotes	development
T619	2164-2174	VBN	denotes	associated
T620	2175-2179	IN	denotes	with
T621	2180-2186	NN	denotes	sterol
T622	2187-2197	NN	denotes	deficiency
T623	2198-2200	IN	denotes	by
T624	2201-2206	NN	denotes	Dhcr7
T625	2207-2211	NN	denotes	loss
T614	2212-2214	VBZ	denotes	is
T626	2215-2220	RB	denotes	still
T627	2221-2228	JJ	denotes	unknown
T628	2228-2230	,	denotes	,
T629	2230-2233	CC	denotes	but
T630	2234-2239	DT	denotes	these
T631	2240-2247	NNS	denotes	results
T633	2248-2250	VBP	denotes	do
T634	2251-2254	RB	denotes	not
T632	2255-2262	VB	denotes	support
T635	2263-2266	DT	denotes	the
T636	2267-2278	NN	denotes	involvement
T637	2279-2281	IN	denotes	of
T638	2282-2294	JJ	denotes	dysregulated
T640	2295-2298	NN	denotes	Shh
T642	2298-2299	HYPH	denotes	-
T641	2299-2306	VBN	denotes	Patched
T644	2306-2307	HYPH	denotes	-
T643	2307-2310	NN	denotes	Gli
T639	2311-2318	NN	denotes	pathway
T645	2319-2321	IN	denotes	in
T646	2322-2329	VBG	denotes	causing
T647	2330-2334	DT	denotes	this
T648	2335-2341	NN	denotes	defect
T649	2341-2342	.	denotes	.
T1160	2355-2366	NN	denotes	Cholesterol
T1161	2367-2369	VBZ	denotes	is
T1162	2370-2371	DT	denotes	a
T1164	2372-2381	JJ	denotes	necessary
T1165	2382-2390	NN	denotes	membrane
T1163	2391-2402	NN	denotes	constituent
T1166	2403-2405	IN	denotes	of
T1167	2406-2409	DT	denotes	all
T1169	2410-2419	JJ	denotes	mammalian
T1170	2419-2421	,	denotes	,
T1171	2421-2428	JJ	denotes	reptile
T1172	2429-2432	CC	denotes	and
T1173	2433-2438	JJ	denotes	avian
T1168	2439-2444	NNS	denotes	cells
T1174	2444-2446	,	denotes	,
T1175	2446-2448	RB	denotes	as
T1177	2449-2453	RB	denotes	well
T1176	2454-2456	IN	denotes	as
T1178	2457-2458	DT	denotes	a
T1180	2459-2462	JJ	denotes	few
T1181	2463-2468	JJ	denotes	other
T1179	2469-2478	NNS	denotes	organisms
T1182	2478-2480	,	denotes	,
T1183	2480-2483	CC	denotes	but
T1184	2484-2487	RB	denotes	not
T1185	2488-2491	DT	denotes	all
T1187	2492-2502	JJ	denotes	eukaryotic
T1186	2503-2512	NNS	denotes	organisms
T1188	2512-2513	.	denotes	.
T1189	2513-2653	sentence	denotes	In the latter cases, such as plants and fungi, cholesterol-like sterols (phytosterols, ergosterol etc.) seem to substitute for cholesterol.
T1190	2514-2516	IN	denotes	In
T1192	2517-2520	DT	denotes	the
T1194	2521-2527	JJ	denotes	latter
T1193	2528-2533	NNS	denotes	cases
T1195	2533-2535	,	denotes	,
T1196	2535-2539	JJ	denotes	such
T1197	2540-2542	IN	denotes	as
T1198	2543-2549	NNS	denotes	plants
T1199	2550-2553	CC	denotes	and
T1200	2554-2559	NNS	denotes	fungi
T1201	2559-2561	,	denotes	,
T1202	2561-2572	NN	denotes	cholesterol
T1204	2572-2573	HYPH	denotes	-
T1203	2573-2577	JJ	denotes	like
T1205	2578-2585	NNS	denotes	sterols
T1206	2586-2587	-LRB-	denotes	(
T1207	2587-2599	NNS	denotes	phytosterols
T1208	2599-2601	,	denotes	,
T1209	2601-2611	NN	denotes	ergosterol
T1210	2612-2616	FW	denotes	etc.
T1211	2616-2617	-RRB-	denotes	)
T1191	2618-2622	VBP	denotes	seem
T1212	2623-2625	TO	denotes	to
T1213	2626-2636	VB	denotes	substitute
T1214	2637-2640	IN	denotes	for
T1215	2641-2652	NN	denotes	cholesterol
T1216	2652-2653	.	denotes	.
T1217	2653-2840	sentence	denotes	The precise need for sterols in biological membranes still remains poorly defined, though its link with microdomains (variously termed rafts, caveoli etc.) may be part of the answer [1].
T1218	2654-2657	DT	denotes	The
T1220	2658-2665	JJ	denotes	precise
T1219	2666-2670	NN	denotes	need
T1222	2671-2674	IN	denotes	for
T1223	2675-2682	NNS	denotes	sterols
T1224	2683-2685	IN	denotes	in
T1225	2686-2696	JJ	denotes	biological
T1226	2697-2706	NNS	denotes	membranes
T1227	2707-2712	RB	denotes	still
T1221	2713-2720	VBZ	denotes	remains
T1228	2721-2727	RB	denotes	poorly
T1229	2728-2735	VBN	denotes	defined
T1230	2735-2737	,	denotes	,
T1231	2737-2743	IN	denotes	though
T1233	2744-2747	PRP$	denotes	its
T1234	2748-2752	NN	denotes	link
T1235	2753-2757	IN	denotes	with
T1236	2758-2770	NNS	denotes	microdomains
T1237	2771-2772	-LRB-	denotes	(
T1238	2772-2781	RB	denotes	variously
T1239	2782-2788	VBN	denotes	termed
T1240	2789-2794	NNS	denotes	rafts
T1241	2794-2796	,	denotes	,
T1242	2796-2803	NNS	denotes	caveoli
T1243	2804-2808	FW	denotes	etc.
T1244	2808-2809	-RRB-	denotes	)
T1245	2810-2813	MD	denotes	may
T1232	2814-2816	VB	denotes	be
T1246	2817-2821	NN	denotes	part
T1247	2822-2824	IN	denotes	of
T1248	2825-2828	DT	denotes	the
T1249	2829-2835	NN	denotes	answer
T1250	2836-2837	-LRB-	denotes	[
T1251	2837-2838	CD	denotes	1
T1252	2838-2839	-RRB-	denotes	]
T1253	2839-2840	.	denotes	.
T1254	2840-3077	sentence	denotes	Although the accumulation of cholesterol in atherosclerotic plaques has led to the concept that 'too much' cholesterol may be deleterious, too little cholesterol has now been proven pathophysiological for abnormal embryonic development.
T1255	2841-2849	IN	denotes	Although
T1257	2850-2853	DT	denotes	the
T1258	2854-2866	NN	denotes	accumulation
T1259	2867-2869	IN	denotes	of
T1260	2870-2881	NN	denotes	cholesterol
T1261	2882-2884	IN	denotes	in
T1262	2885-2900	JJ	denotes	atherosclerotic
T1263	2901-2908	NNS	denotes	plaques
T1264	2909-2912	VBZ	denotes	has
T1256	2913-2916	VBN	denotes	led
T1266	2917-2919	IN	denotes	to
T1267	2920-2923	DT	denotes	the
T1268	2924-2931	NN	denotes	concept
T1269	2932-2936	IN	denotes	that
T1271	2937-2938	``	denotes	'
T1273	2938-2941	RB	denotes	too
T1272	2942-2946	JJ	denotes	much
T1275	2946-2947	''	denotes	'
T1274	2948-2959	NN	denotes	cholesterol
T1276	2960-2963	MD	denotes	may
T1270	2964-2966	VB	denotes	be
T1277	2967-2978	JJ	denotes	deleterious
T1278	2978-2980	,	denotes	,
T1279	2980-2983	RB	denotes	too
T1281	2984-2990	JJ	denotes	little
T1280	2991-3002	NN	denotes	cholesterol
T1282	3003-3006	VBZ	denotes	has
T1283	3007-3010	RB	denotes	now
T1284	3011-3015	VBN	denotes	been
T1265	3016-3022	VBN	denotes	proven
T1285	3023-3041	JJ	denotes	pathophysiological
T1286	3042-3045	IN	denotes	for
T1287	3046-3054	JJ	denotes	abnormal
T1289	3055-3064	JJ	denotes	embryonic
T1288	3065-3076	NN	denotes	development
T1290	3076-3077	.	denotes	.
T1291	3077-3313	sentence	denotes	Smith-Lemli-Opitz syndrome (SLOS, MIM 270400), a relative common dysmorphology disorder, is caused by mutations in DHCR7 [2-5], which encodes for 7-dehydrocholesterol Δ7-reductase and catalyzes a final step of cholesterol biosynthesis.
T1292	3078-3083	NNP	denotes	Smith
T1294	3083-3084	HYPH	denotes	-
T1295	3084-3089	NNP	denotes	Lemli
T1296	3089-3090	HYPH	denotes	-
T1293	3090-3095	NNP	denotes	Opitz
T1297	3096-3104	NN	denotes	syndrome
T1299	3105-3106	-LRB-	denotes	(
T1300	3106-3110	NN	denotes	SLOS
T1301	3110-3112	,	denotes	,
T1302	3112-3115	NN	denotes	MIM
T1303	3116-3122	CD	denotes	270400
T1304	3122-3123	-RRB-	denotes	)
T1305	3123-3125	,	denotes	,
T1306	3125-3126	DT	denotes	a
T1308	3127-3135	JJ	denotes	relative
T1309	3136-3142	JJ	denotes	common
T1310	3143-3156	NN	denotes	dysmorphology
T1307	3157-3165	NN	denotes	disorder
T1311	3165-3167	,	denotes	,
T1312	3167-3169	VBZ	denotes	is
T1298	3170-3176	VBN	denotes	caused
T1313	3177-3179	IN	denotes	by
T1314	3180-3189	NNS	denotes	mutations
T1315	3190-3192	IN	denotes	in
T1316	3193-3198	NN	denotes	DHCR7
T1317	3199-3200	-LRB-	denotes	[
T1318	3200-3201	CD	denotes	2
T1319	3201-3202	SYM	denotes	-
T1320	3202-3203	CD	denotes	5
T1321	3203-3204	-RRB-	denotes	]
T1322	3204-3206	,	denotes	,
T1323	3206-3211	WDT	denotes	which
T1324	3212-3219	VBZ	denotes	encodes
T1325	3220-3223	IN	denotes	for
T1326	3224-3225	CD	denotes	7
T1328	3225-3226	HYPH	denotes	-
T1327	3226-3244	NN	denotes	dehydrocholesterol
T1330	3245-3247	NN	denotes	Δ7
T1331	3247-3248	HYPH	denotes	-
T1329	3248-3257	NN	denotes	reductase
T1332	3258-3261	CC	denotes	and
T1333	3262-3271	VBZ	denotes	catalyzes
T1334	3272-3273	DT	denotes	a
T1336	3274-3279	JJ	denotes	final
T1335	3280-3284	NN	denotes	step
T1337	3285-3287	IN	denotes	of
T1338	3288-3299	NN	denotes	cholesterol
T1339	3300-3312	NN	denotes	biosynthesis
T1340	3312-3313	.	denotes	.
T1341	3313-3567	sentence	denotes	Recognition of other dysmorphology syndromes caused by defects in the post-squalene cholesterol biosynthesis pathway, such as desmosterolosis, lathosterolosis, X-linked chondrodysplasia punctanta and CHILD syndrome [6-15], has strengthened this concept.
T1342	3314-3325	NN	denotes	Recognition
T1344	3326-3328	IN	denotes	of
T1345	3329-3334	JJ	denotes	other
T1347	3335-3348	NN	denotes	dysmorphology
T1346	3349-3358	NNS	denotes	syndromes
T1348	3359-3365	VBN	denotes	caused
T1349	3366-3368	IN	denotes	by
T1350	3369-3376	NNS	denotes	defects
T1351	3377-3379	IN	denotes	in
T1352	3380-3383	DT	denotes	the
T1354	3384-3397	JJ	denotes	post-squalene
T1355	3398-3409	NN	denotes	cholesterol
T1356	3410-3422	NN	denotes	biosynthesis
T1353	3423-3430	NN	denotes	pathway
T1357	3430-3432	,	denotes	,
T1358	3432-3436	JJ	denotes	such
T1359	3437-3439	IN	denotes	as
T1360	3440-3455	NN	denotes	desmosterolosis
T1361	3455-3457	,	denotes	,
T1362	3457-3472	NN	denotes	lathosterolosis
T1363	3472-3474	,	denotes	,
T1364	3474-3475	NN	denotes	X
T1366	3475-3476	HYPH	denotes	-
T1365	3476-3482	VBN	denotes	linked
T1368	3483-3499	NN	denotes	chondrodysplasia
T1367	3500-3509	NN	denotes	punctanta
T1369	3510-3513	CC	denotes	and
T1370	3514-3519	NN	denotes	CHILD
T1371	3520-3528	NN	denotes	syndrome
T1372	3529-3530	-LRB-	denotes	[
T1373	3530-3531	CD	denotes	6
T1374	3531-3532	SYM	denotes	-
T1375	3532-3534	CD	denotes	15
T1376	3534-3535	-RRB-	denotes	]
T1377	3535-3537	,	denotes	,
T1378	3537-3540	VBZ	denotes	has
T1343	3541-3553	VBN	denotes	strengthened
T1379	3554-3558	DT	denotes	this
T1380	3559-3566	NN	denotes	concept
T1381	3566-3567	.	denotes	.
T1382	3567-3635	sentence	denotes	Genetic disruption of the Dhcr7 results in neonatal lethality [16].
T1383	3568-3575	JJ	denotes	Genetic
T1384	3576-3586	NN	denotes	disruption
T1386	3587-3589	IN	denotes	of
T1387	3590-3593	DT	denotes	the
T1388	3594-3599	NN	denotes	Dhcr7
T1385	3600-3607	VBZ	denotes	results
T1389	3608-3610	IN	denotes	in
T1390	3611-3619	JJ	denotes	neonatal
T1391	3620-3629	NN	denotes	lethality
T1392	3630-3631	-LRB-	denotes	[
T1393	3631-3633	CD	denotes	16
T1394	3633-3634	-RRB-	denotes	]
T1395	3634-3635	.	denotes	.
T1396	3635-3809	sentence	denotes	Neonatal mice homozygous for the Dhcr7 gene disruption are deficient in cholesterol and have increased accumulation of the cholesterol precursor 7-dehydrocholesterol (7DHC).
T1397	3636-3644	JJ	denotes	Neonatal
T1398	3645-3649	NNS	denotes	mice
T1400	3650-3660	JJ	denotes	homozygous
T1401	3661-3664	IN	denotes	for
T1402	3665-3668	DT	denotes	the
T1404	3669-3674	NN	denotes	Dhcr7
T1405	3675-3679	NN	denotes	gene
T1403	3680-3690	NN	denotes	disruption
T1399	3691-3694	VBP	denotes	are
T1406	3695-3704	JJ	denotes	deficient
T1407	3705-3707	IN	denotes	in
T1408	3708-3719	NN	denotes	cholesterol
T1409	3720-3723	CC	denotes	and
T1410	3724-3728	VBP	denotes	have
T1411	3729-3738	VBN	denotes	increased
T1412	3739-3751	NN	denotes	accumulation
T1413	3752-3754	IN	denotes	of
T1414	3755-3758	DT	denotes	the
T1416	3759-3770	NN	denotes	cholesterol
T1415	3771-3780	NN	denotes	precursor
T1417	3781-3782	CD	denotes	7
T1419	3782-3783	HYPH	denotes	-
T1418	3783-3801	NN	denotes	dehydrocholesterol
T1420	3802-3803	-LRB-	denotes	(
T1421	3803-3807	NN	denotes	7DHC
T1422	3807-3808	-RRB-	denotes	)
T1423	3808-3809	.	denotes	.
T1424	3809-3956	sentence	denotes	Although no embryonic lethality was noted, these knockout pups exhibited a number of developmental abnormalities and died within 24 h after birth.
T1425	3810-3818	IN	denotes	Although
T1427	3819-3821	DT	denotes	no
T1429	3822-3831	JJ	denotes	embryonic
T1428	3832-3841	NN	denotes	lethality
T1430	3842-3845	VBD	denotes	was
T1426	3846-3851	VBN	denotes	noted
T1432	3851-3853	,	denotes	,
T1433	3853-3858	DT	denotes	these
T1435	3859-3867	NN	denotes	knockout
T1434	3868-3872	NNS	denotes	pups
T1431	3873-3882	VBD	denotes	exhibited
T1436	3883-3884	DT	denotes	a
T1437	3885-3891	NN	denotes	number
T1438	3892-3894	IN	denotes	of
T1439	3895-3908	JJ	denotes	developmental
T1440	3909-3922	NNS	denotes	abnormalities
T1441	3923-3926	CC	denotes	and
T1442	3927-3931	VBD	denotes	died
T1443	3932-3938	IN	denotes	within
T1444	3939-3941	CD	denotes	24
T1445	3942-3943	NN	denotes	h
T1446	3944-3949	IN	denotes	after
T1447	3950-3955	NN	denotes	birth
T1448	3955-3956	.	denotes	.
T1449	3956-4172	sentence	denotes	A similar mouse SLOS model, generated by replacing exons 3, 4 and part of exon 5 of the murine Dhcr7 gene, with similar biochemical and structural defects and a 100 % neonatal lethality, has also been reported [17].
T1450	3957-3958	DT	denotes	A
T1452	3959-3966	JJ	denotes	similar
T1453	3967-3972	NN	denotes	mouse
T1454	3973-3977	NN	denotes	SLOS
T1451	3978-3983	NN	denotes	model
T1456	3983-3985	,	denotes	,
T1457	3985-3994	VBN	denotes	generated
T1458	3995-3997	IN	denotes	by
T1459	3998-4007	VBG	denotes	replacing
T1460	4008-4013	NNS	denotes	exons
T1462	4014-4015	CD	denotes	3
T1463	4015-4017	,	denotes	,
T1461	4017-4018	CD	denotes	4
T1464	4019-4022	CC	denotes	and
T1465	4023-4027	NN	denotes	part
T1466	4028-4030	IN	denotes	of
T1467	4031-4035	NN	denotes	exon
T1468	4036-4037	CD	denotes	5
T1469	4038-4040	IN	denotes	of
T1470	4041-4044	DT	denotes	the
T1472	4045-4051	JJ	denotes	murine
T1473	4052-4057	NN	denotes	Dhcr7
T1471	4058-4062	NN	denotes	gene
T1474	4062-4064	,	denotes	,
T1475	4064-4068	IN	denotes	with
T1476	4069-4076	JJ	denotes	similar
T1478	4077-4088	JJ	denotes	biochemical
T1479	4089-4092	CC	denotes	and
T1480	4093-4103	JJ	denotes	structural
T1477	4104-4111	NNS	denotes	defects
T1481	4112-4115	CC	denotes	and
T1482	4116-4117	DT	denotes	a
T1484	4118-4121	CD	denotes	100
T1485	4122-4123	NN	denotes	%
T1486	4124-4132	JJ	denotes	neonatal
T1483	4133-4142	NN	denotes	lethality
T1487	4142-4144	,	denotes	,
T1488	4144-4147	VBZ	denotes	has
T1489	4148-4152	RB	denotes	also
T1490	4153-4157	VBN	denotes	been
T1455	4158-4166	VBN	denotes	reported
T1491	4167-4168	-LRB-	denotes	[
T1492	4168-4170	CD	denotes	17
T1493	4170-4171	-RRB-	denotes	]
T1494	4171-4172	.	denotes	.
T1495	4172-4432	sentence	denotes	Poor lung development or diffuse lung alveolar atelectasis with cyanotic and lethargic breathing are consistent between both Dhcr7 null models [16,17], and therefore is the likely reason for the respiratory insufficiency and subsequent death of Dhcr7-/- pups.
T1496	4173-4177	JJ	denotes	Poor
T1498	4178-4182	NN	denotes	lung
T1497	4183-4194	NN	denotes	development
T1500	4195-4197	CC	denotes	or
T1501	4198-4205	JJ	denotes	diffuse
T1503	4206-4210	NN	denotes	lung
T1504	4211-4219	JJ	denotes	alveolar
T1502	4220-4231	NN	denotes	atelectasis
T1505	4232-4236	IN	denotes	with
T1506	4237-4245	JJ	denotes	cyanotic
T1508	4246-4249	CC	denotes	and
T1509	4250-4259	JJ	denotes	lethargic
T1507	4260-4269	NN	denotes	breathing
T1499	4270-4273	VBP	denotes	are
T1510	4274-4284	JJ	denotes	consistent
T1511	4285-4292	IN	denotes	between
T1512	4293-4297	CC	denotes	both
T1514	4298-4303	NN	denotes	Dhcr7
T1515	4304-4308	JJ	denotes	null
T1513	4309-4315	NNS	denotes	models
T1516	4316-4317	-LRB-	denotes	[
T1518	4317-4319	CD	denotes	16
T1519	4319-4320	,	denotes	,
T1517	4320-4322	CD	denotes	17
T1520	4322-4323	-RRB-	denotes	]
T1521	4323-4325	,	denotes	,
T1522	4325-4328	CC	denotes	and
T1523	4329-4338	RB	denotes	therefore
T1524	4339-4341	VBZ	denotes	is
T1525	4342-4345	DT	denotes	the
T1527	4346-4352	JJ	denotes	likely
T1526	4353-4359	NN	denotes	reason
T1528	4360-4363	IN	denotes	for
T1529	4364-4367	DT	denotes	the
T1531	4368-4379	JJ	denotes	respiratory
T1530	4380-4393	NN	denotes	insufficiency
T1532	4394-4397	CC	denotes	and
T1533	4398-4408	JJ	denotes	subsequent
T1534	4409-4414	NN	denotes	death
T1535	4415-4417	IN	denotes	of
T1536	4418-4423	NN	denotes	Dhcr7
T1538	4423-4424	SYM	denotes	-
T1539	4424-4425	SYM	denotes	/
T1540	4425-4426	SYM	denotes	-
T1537	4427-4431	NNS	denotes	pups
T1541	4431-4432	.	denotes	.
T1542	4432-4636	sentence	denotes	These Dhcr7 knockout mice are models for human SLOS, especially the more severely affected patients, since early postnatal lethality with respiratory failure has been reported in severe SLOS cases [4,5].
T1543	4433-4438	DT	denotes	These
T1545	4439-4444	NN	denotes	Dhcr7
T1546	4445-4453	NN	denotes	knockout
T1544	4454-4458	NNS	denotes	mice
T1547	4459-4462	VBP	denotes	are
T1548	4463-4469	NNS	denotes	models
T1549	4470-4473	IN	denotes	for
T1550	4474-4479	JJ	denotes	human
T1551	4480-4484	NN	denotes	SLOS
T1552	4484-4486	,	denotes	,
T1553	4486-4496	RB	denotes	especially
T1555	4497-4500	DT	denotes	the
T1556	4501-4505	RBR	denotes	more
T1557	4506-4514	RB	denotes	severely
T1558	4515-4523	VBN	denotes	affected
T1554	4524-4532	NNS	denotes	patients
T1559	4532-4534	,	denotes	,
T1560	4534-4539	IN	denotes	since
T1562	4540-4545	JJ	denotes	early
T1563	4546-4555	JJ	denotes	postnatal
T1564	4556-4565	NN	denotes	lethality
T1565	4566-4570	IN	denotes	with
T1566	4571-4582	JJ	denotes	respiratory
T1567	4583-4590	NN	denotes	failure
T1568	4591-4594	VBZ	denotes	has
T1569	4595-4599	VBN	denotes	been
T1561	4600-4608	VBN	denotes	reported
T1570	4609-4611	IN	denotes	in
T1571	4612-4618	JJ	denotes	severe
T1573	4619-4623	NN	denotes	SLOS
T1572	4624-4629	NNS	denotes	cases
T1574	4630-4631	-LRB-	denotes	[
T1576	4631-4632	CD	denotes	4
T1577	4632-4633	,	denotes	,
T1575	4633-4634	CD	denotes	5
T1578	4634-4635	-RRB-	denotes	]
T1579	4635-4636	.	denotes	.
T1580	4636-4852	sentence	denotes	Developmental lung abnormalities are also relatively common in SLOS patients, including pulmonary hypoplasia, abnormal pulmonary lobation and pulmonary arteries, anomalies of laryngeal and tracheal development [18].
T1581	4637-4650	JJ	denotes	Developmental
T1583	4651-4655	NN	denotes	lung
T1582	4656-4669	NNS	denotes	abnormalities
T1584	4670-4673	VBP	denotes	are
T1585	4674-4678	RB	denotes	also
T1586	4679-4689	RB	denotes	relatively
T1587	4690-4696	JJ	denotes	common
T1588	4697-4699	IN	denotes	in
T1589	4700-4704	NN	denotes	SLOS
T1590	4705-4713	NNS	denotes	patients
T1591	4713-4715	,	denotes	,
T1592	4715-4724	VBG	denotes	including
T1593	4725-4734	JJ	denotes	pulmonary
T1594	4735-4745	NN	denotes	hypoplasia
T1595	4745-4747	,	denotes	,
T1596	4747-4755	JJ	denotes	abnormal
T1598	4756-4765	JJ	denotes	pulmonary
T1597	4766-4774	NN	denotes	lobation
T1599	4775-4778	CC	denotes	and
T1600	4779-4788	JJ	denotes	pulmonary
T1601	4789-4797	NNS	denotes	arteries
T1602	4797-4799	,	denotes	,
T1603	4799-4808	NNS	denotes	anomalies
T1604	4809-4811	IN	denotes	of
T1605	4812-4821	JJ	denotes	laryngeal
T1607	4822-4825	CC	denotes	and
T1608	4826-4834	JJ	denotes	tracheal
T1606	4835-4846	NN	denotes	development
T1609	4847-4848	-LRB-	denotes	[
T1610	4848-4850	CD	denotes	18
T1611	4850-4851	-RRB-	denotes	]
T1612	4851-4852	.	denotes	.
T1613	4852-5053	sentence	denotes	Mammalian lung is unique in that it is fully developed, but does not function for gas exchange until at the moment of birth; the majority of structural development and maturation takes place in utero.
T1614	4853-4862	JJ	denotes	Mammalian
T1615	4863-4867	NN	denotes	lung
T1616	4868-4870	VBZ	denotes	is
T1618	4871-4877	JJ	denotes	unique
T1619	4878-4880	IN	denotes	in
T1621	4881-4885	IN	denotes	that
T1622	4886-4888	PRP	denotes	it
T1620	4889-4891	VBZ	denotes	is
T1623	4892-4897	RB	denotes	fully
T1624	4898-4907	JJ	denotes	developed
T1625	4907-4909	,	denotes	,
T1626	4909-4912	CC	denotes	but
T1627	4913-4917	VBZ	denotes	does
T1629	4918-4921	RB	denotes	not
T1628	4922-4930	VB	denotes	function
T1630	4931-4934	IN	denotes	for
T1631	4935-4938	NN	denotes	gas
T1632	4939-4947	NN	denotes	exchange
T1633	4948-4953	IN	denotes	until
T1634	4954-4956	IN	denotes	at
T1635	4957-4960	DT	denotes	the
T1636	4961-4967	NN	denotes	moment
T1637	4968-4970	IN	denotes	of
T1638	4971-4976	NN	denotes	birth
T1639	4976-4977	:	denotes	;
T1640	4978-4981	DT	denotes	the
T1641	4982-4990	NN	denotes	majority
T1642	4991-4993	IN	denotes	of
T1643	4994-5004	JJ	denotes	structural
T1644	5005-5016	NN	denotes	development
T1645	5017-5020	CC	denotes	and
T1646	5021-5031	NN	denotes	maturation
T1617	5032-5037	VBZ	denotes	takes
T1647	5038-5043	NN	denotes	place
T1648	5044-5046	FW	denotes	in
T1649	5047-5052	FW	denotes	utero
T1650	5052-5053	.	denotes	.
T1651	5053-5265	sentence	denotes	Lung development is a complex process that involves branching morphogenesis of epithelium, interstitial development including vasculogenesis, cellular differentiation, biochemical maturation and physical growth.
T1652	5054-5058	NN	denotes	Lung
T1653	5059-5070	NN	denotes	development
T1654	5071-5073	VBZ	denotes	is
T1655	5074-5075	DT	denotes	a
T1657	5076-5083	JJ	denotes	complex
T1656	5084-5091	NN	denotes	process
T1658	5092-5096	WDT	denotes	that
T1659	5097-5105	VBZ	denotes	involves
T1660	5106-5115	NN	denotes	branching
T1661	5116-5129	NN	denotes	morphogenesis
T1662	5130-5132	IN	denotes	of
T1663	5133-5143	NN	denotes	epithelium
T1664	5143-5145	,	denotes	,
T1665	5145-5157	JJ	denotes	interstitial
T1666	5158-5169	NN	denotes	development
T1667	5170-5179	VBG	denotes	including
T1668	5180-5194	NN	denotes	vasculogenesis
T1669	5194-5196	,	denotes	,
T1670	5196-5204	JJ	denotes	cellular
T1671	5205-5220	NN	denotes	differentiation
T1672	5220-5222	,	denotes	,
T1673	5222-5233	JJ	denotes	biochemical
T1674	5234-5244	NN	denotes	maturation
T1675	5245-5248	CC	denotes	and
T1676	5249-5257	JJ	denotes	physical
T1677	5258-5264	NN	denotes	growth
T1678	5264-5265	.	denotes	.
T1679	5265-5447	sentence	denotes	Four stages of mouse lung development have been described, based upon the histological appearances of lung; pseudoglandular, canalicular, terminal saccular and alveolar stages [19].
T1680	5266-5270	CD	denotes	Four
T1681	5271-5277	NNS	denotes	stages
T1683	5278-5280	IN	denotes	of
T1684	5281-5286	NN	denotes	mouse
T1686	5287-5291	NN	denotes	lung
T1685	5292-5303	NN	denotes	development
T1687	5304-5308	VBP	denotes	have
T1688	5309-5313	VBN	denotes	been
T1682	5314-5323	VBN	denotes	described
T1689	5323-5325	,	denotes	,
T1690	5325-5330	VBN	denotes	based
T1691	5331-5335	IN	denotes	upon
T1692	5336-5339	DT	denotes	the
T1694	5340-5352	JJ	denotes	histological
T1693	5353-5364	NNS	denotes	appearances
T1695	5365-5367	IN	denotes	of
T1696	5368-5372	NN	denotes	lung
T1697	5372-5373	:	denotes	;
T1698	5374-5389	JJ	denotes	pseudoglandular
T1700	5389-5391	,	denotes	,
T1701	5391-5402	JJ	denotes	canalicular
T1702	5402-5404	,	denotes	,
T1703	5404-5412	JJ	denotes	terminal
T1704	5413-5421	JJ	denotes	saccular
T1705	5422-5425	CC	denotes	and
T1706	5426-5434	JJ	denotes	alveolar
T1699	5435-5441	NNS	denotes	stages
T1707	5442-5443	-LRB-	denotes	[
T1708	5443-5445	CD	denotes	19
T1709	5445-5446	-RRB-	denotes	]
T1710	5446-5447	.	denotes	.
T1711	5447-5547	sentence	denotes	Impaired hedgehog function has been proposed as a mechanism underlying malformations found in SLOS.
T1712	5448-5456	JJ	denotes	Impaired
T1714	5457-5465	NN	denotes	hedgehog
T1713	5466-5474	NN	denotes	function
T1716	5475-5478	VBZ	denotes	has
T1717	5479-5483	VBN	denotes	been
T1715	5484-5492	VBN	denotes	proposed
T1718	5493-5495	IN	denotes	as
T1719	5496-5497	DT	denotes	a
T1720	5498-5507	NN	denotes	mechanism
T1721	5508-5518	VBG	denotes	underlying
T1722	5519-5532	NNS	denotes	malformations
T1723	5533-5538	VBN	denotes	found
T1724	5539-5541	IN	denotes	in
T1725	5542-5546	NN	denotes	SLOS
T1726	5546-5547	.	denotes	.
T1727	5547-5689	sentence	denotes	Hedgehog family members play diverse roles in embryonic development, and cholesterol is necessary for maturation of these morphogens [20-23].
T1728	5548-5556	NN	denotes	Hedgehog
T1730	5557-5563	NN	denotes	family
T1729	5564-5571	NNS	denotes	members
T1731	5572-5576	VBP	denotes	play
T1732	5577-5584	JJ	denotes	diverse
T1733	5585-5590	NNS	denotes	roles
T1734	5591-5593	IN	denotes	in
T1735	5594-5603	JJ	denotes	embryonic
T1736	5604-5615	NN	denotes	development
T1737	5615-5617	,	denotes	,
T1738	5617-5620	CC	denotes	and
T1739	5621-5632	NN	denotes	cholesterol
T1740	5633-5635	VBZ	denotes	is
T1741	5636-5645	JJ	denotes	necessary
T1742	5646-5649	IN	denotes	for
T1743	5650-5660	NN	denotes	maturation
T1744	5661-5663	IN	denotes	of
T1745	5664-5669	DT	denotes	these
T1746	5670-5680	NNS	denotes	morphogens
T1747	5681-5682	-LRB-	denotes	[
T1748	5682-5684	CD	denotes	20
T1749	5684-5685	SYM	denotes	-
T1750	5685-5687	CD	denotes	23
T1751	5687-5688	-RRB-	denotes	]
T1752	5688-5689	.	denotes	.
T1753	5689-5799	sentence	denotes	The sonic hedgehog (Shh) signaling cascade has been shown to play a central role in lung development [24-26].
T1754	5690-5693	DT	denotes	The
T1756	5694-5699	JJ	denotes	sonic
T1757	5700-5708	NN	denotes	hedgehog
T1758	5709-5710	-LRB-	denotes	(
T1759	5710-5713	NN	denotes	Shh
T1760	5713-5714	-RRB-	denotes	)
T1761	5715-5724	NN	denotes	signaling
T1755	5725-5732	NN	denotes	cascade
T1763	5733-5736	VBZ	denotes	has
T1764	5737-5741	VBN	denotes	been
T1762	5742-5747	VBN	denotes	shown
T1765	5748-5750	TO	denotes	to
T1766	5751-5755	VB	denotes	play
T1767	5756-5757	DT	denotes	a
T1769	5758-5765	JJ	denotes	central
T1768	5766-5770	NN	denotes	role
T1770	5771-5773	IN	denotes	in
T1771	5774-5778	NN	denotes	lung
T1772	5779-5790	NN	denotes	development
T1773	5791-5792	-LRB-	denotes	[
T1774	5792-5794	CD	denotes	24
T1775	5794-5795	SYM	denotes	-
T1776	5795-5797	CD	denotes	26
T1777	5797-5798	-RRB-	denotes	]
T1778	5798-5799	.	denotes	.
T1779	5799-5975	sentence	denotes	Loss of Shh function resulted in severe lung defects associated with loss of branching morphogenesis, but preserved proximal-to-distal differentiation of lung epithelium [24].
T1780	5800-5804	NN	denotes	Loss
T1782	5805-5807	IN	denotes	of
T1783	5808-5811	NN	denotes	Shh
T1784	5812-5820	NN	denotes	function
T1781	5821-5829	VBD	denotes	resulted
T1785	5830-5832	IN	denotes	in
T1786	5833-5839	JJ	denotes	severe
T1788	5840-5844	NN	denotes	lung
T1787	5845-5852	NNS	denotes	defects
T1789	5853-5863	VBN	denotes	associated
T1790	5864-5868	IN	denotes	with
T1791	5869-5873	NN	denotes	loss
T1792	5874-5876	IN	denotes	of
T1793	5877-5886	VBG	denotes	branching
T1794	5887-5900	NN	denotes	morphogenesis
T1795	5900-5902	,	denotes	,
T1796	5902-5905	CC	denotes	but
T1797	5906-5915	VBN	denotes	preserved
T1798	5916-5924	JJ	denotes	proximal
T1800	5924-5925	HYPH	denotes	-
T1801	5925-5927	IN	denotes	to
T1802	5927-5928	HYPH	denotes	-
T1803	5928-5934	JJ	denotes	distal
T1799	5935-5950	NN	denotes	differentiation
T1804	5951-5953	IN	denotes	of
T1805	5954-5958	NN	denotes	lung
T1806	5959-5969	NN	denotes	epithelium
T1807	5970-5971	-LRB-	denotes	[
T1808	5971-5973	CD	denotes	24
T1809	5973-5974	-RRB-	denotes	]
T1810	5974-5975	.	denotes	.
T1811	5975-6195	sentence	denotes	Overexpression of Shh in the distal lung epithelium resulted in the absence of functional alveoli and an increase in interstitial tissue caused by an increased proliferation of both epithelial and mesenchyme cells [26].
T1812	5976-5990	NN	denotes	Overexpression
T1814	5991-5993	IN	denotes	of
T1815	5994-5997	NN	denotes	Shh
T1816	5998-6000	IN	denotes	in
T1817	6001-6004	DT	denotes	the
T1819	6005-6011	JJ	denotes	distal
T1820	6012-6016	NN	denotes	lung
T1818	6017-6027	NN	denotes	epithelium
T1813	6028-6036	VBD	denotes	resulted
T1821	6037-6039	IN	denotes	in
T1822	6040-6043	DT	denotes	the
T1823	6044-6051	NN	denotes	absence
T1824	6052-6054	IN	denotes	of
T1825	6055-6065	JJ	denotes	functional
T1826	6066-6073	NNS	denotes	alveoli
T1827	6074-6077	CC	denotes	and
T1828	6078-6080	DT	denotes	an
T1829	6081-6089	NN	denotes	increase
T1830	6090-6092	IN	denotes	in
T1831	6093-6105	JJ	denotes	interstitial
T1832	6106-6112	NN	denotes	tissue
T1833	6113-6119	VBN	denotes	caused
T1834	6120-6122	IN	denotes	by
T1835	6123-6125	DT	denotes	an
T1837	6126-6135	VBN	denotes	increased
T1836	6136-6149	NN	denotes	proliferation
T1838	6150-6152	IN	denotes	of
T1839	6153-6157	CC	denotes	both
T1840	6158-6168	JJ	denotes	epithelial
T1842	6169-6172	CC	denotes	and
T1843	6173-6183	NN	denotes	mesenchyme
T1841	6184-6189	NNS	denotes	cells
T1844	6190-6191	-LRB-	denotes	[
T1845	6191-6193	CD	denotes	26
T1846	6193-6194	-RRB-	denotes	]
T1847	6194-6195	.	denotes	.
T1848	6195-6296	sentence	denotes	Mice with Shh overexpression died less than 24 h after birth, probably owing to respiratory failure.
T1849	6196-6200	NNS	denotes	Mice
T1851	6201-6205	IN	denotes	with
T1852	6206-6209	NN	denotes	Shh
T1853	6210-6224	NN	denotes	overexpression
T1850	6225-6229	VBD	denotes	died
T1854	6230-6234	JJR	denotes	less
T1856	6235-6239	IN	denotes	than
T1855	6240-6242	CD	denotes	24
T1857	6243-6244	NNS	denotes	h
T1858	6245-6250	IN	denotes	after
T1859	6251-6256	NN	denotes	birth
T1860	6256-6258	,	denotes	,
T1861	6258-6266	RB	denotes	probably
T1862	6267-6272	VBG	denotes	owing
T1863	6273-6275	IN	denotes	to
T1864	6276-6287	JJ	denotes	respiratory
T1865	6288-6295	NN	denotes	failure
T1866	6295-6296	.	denotes	.
T1867	6296-6594	sentence	denotes	It has been reported that inhibition of cholesterol biosynthesis by chemical inhibitors of Dhcr7 also led to a disturbance of the Shh signaling with abnormal embryogenesis [27,28], and severe sterol deprivation can indeed inhibit the processing of transfected Shh in cultured mammalian cells [29].
T1868	6297-6299	PRP	denotes	It
T1870	6300-6303	VBZ	denotes	has
T1871	6304-6308	VBN	denotes	been
T1869	6309-6317	VBN	denotes	reported
T1872	6318-6322	IN	denotes	that
T1874	6323-6333	NN	denotes	inhibition
T1875	6334-6336	IN	denotes	of
T1876	6337-6348	NN	denotes	cholesterol
T1877	6349-6361	NN	denotes	biosynthesis
T1878	6362-6364	IN	denotes	by
T1879	6365-6373	JJ	denotes	chemical
T1880	6374-6384	NNS	denotes	inhibitors
T1881	6385-6387	IN	denotes	of
T1882	6388-6393	NN	denotes	Dhcr7
T1883	6394-6398	RB	denotes	also
T1873	6399-6402	VBD	denotes	led
T1884	6403-6405	IN	denotes	to
T1885	6406-6407	DT	denotes	a
T1886	6408-6419	NN	denotes	disturbance
T1887	6420-6422	IN	denotes	of
T1888	6423-6426	DT	denotes	the
T1890	6427-6430	NN	denotes	Shh
T1889	6431-6440	NN	denotes	signaling
T1891	6441-6445	IN	denotes	with
T1892	6446-6454	JJ	denotes	abnormal
T1893	6455-6468	NN	denotes	embryogenesis
T1894	6469-6470	-LRB-	denotes	[
T1896	6470-6472	CD	denotes	27
T1897	6472-6473	,	denotes	,
T1895	6473-6475	CD	denotes	28
T1898	6475-6476	-RRB-	denotes	]
T1899	6476-6478	,	denotes	,
T1900	6478-6481	CC	denotes	and
T1901	6482-6488	JJ	denotes	severe
T1903	6489-6495	NN	denotes	sterol
T1902	6496-6507	NN	denotes	deprivation
T1905	6508-6511	MD	denotes	can
T1906	6512-6518	RB	denotes	indeed
T1904	6519-6526	VB	denotes	inhibit
T1907	6527-6530	DT	denotes	the
T1908	6531-6541	NN	denotes	processing
T1909	6542-6544	IN	denotes	of
T1910	6545-6556	VBN	denotes	transfected
T1911	6557-6560	NN	denotes	Shh
T1912	6561-6563	IN	denotes	in
T1913	6564-6572	VBN	denotes	cultured
T1915	6573-6582	JJ	denotes	mammalian
T1914	6583-6588	NNS	denotes	cells
T1916	6589-6590	-LRB-	denotes	[
T1917	6590-6592	CD	denotes	29
T1918	6592-6593	-RRB-	denotes	]
T1919	6593-6594	.	denotes	.
T1920	6594-6693	sentence	denotes	In the present studies, we characterized lung development in Dhcr7-/- embryos from E10.5 to birth.
T1921	6595-6597	IN	denotes	In
T1923	6598-6601	DT	denotes	the
T1925	6602-6609	JJ	denotes	present
T1924	6610-6617	NNS	denotes	studies
T1926	6617-6619	,	denotes	,
T1927	6619-6621	PRP	denotes	we
T1922	6622-6635	VBD	denotes	characterized
T1928	6636-6640	NN	denotes	lung
T1929	6641-6652	NN	denotes	development
T1930	6653-6655	IN	denotes	in
T1931	6656-6661	NN	denotes	Dhcr7
T1933	6661-6662	SYM	denotes	-
T1934	6662-6663	SYM	denotes	/
T1935	6663-6664	SYM	denotes	-
T1932	6665-6672	NNS	denotes	embryos
T1936	6673-6677	IN	denotes	from
T1937	6678-6683	NN	denotes	E10.5
T1938	6684-6686	IN	denotes	to
T1939	6687-6692	NN	denotes	birth
T1940	6692-6693	.	denotes	.
T1941	6693-6861	sentence	denotes	Our results showed that cholesterol deficiency, caused by loss of Dhcr7 activity, did not alter lung branching morphogenesis (early gestational stages, E9.5 to E14.5).
T1942	6694-6697	PRP$	denotes	Our
T1943	6698-6705	NNS	denotes	results
T1944	6706-6712	VBD	denotes	showed
T1945	6713-6717	IN	denotes	that
T1947	6718-6729	NN	denotes	cholesterol
T1948	6730-6740	NN	denotes	deficiency
T1949	6740-6742	,	denotes	,
T1950	6742-6748	VBN	denotes	caused
T1951	6749-6751	IN	denotes	by
T1952	6752-6756	NN	denotes	loss
T1953	6757-6759	IN	denotes	of
T1954	6760-6765	NN	denotes	Dhcr7
T1955	6766-6774	NN	denotes	activity
T1956	6774-6776	,	denotes	,
T1957	6776-6779	VBD	denotes	did
T1958	6780-6783	RB	denotes	not
T1946	6784-6789	VB	denotes	alter
T1959	6790-6794	NN	denotes	lung
T1960	6795-6804	NN	denotes	branching
T1961	6805-6818	NN	denotes	morphogenesis
T1962	6819-6820	-LRB-	denotes	(
T1964	6820-6825	JJ	denotes	early
T1965	6826-6837	JJ	denotes	gestational
T1963	6838-6844	NNS	denotes	stages
T1966	6844-6846	,	denotes	,
T1967	6846-6850	NN	denotes	E9.5
T1968	6851-6853	IN	denotes	to
T1969	6854-6859	NN	denotes	E14.5
T1970	6859-6860	-RRB-	denotes	)
T1971	6860-6861	.	denotes	.
T1972	6861-7000	sentence	denotes	However, lung saccular development was impaired, with arrested or partially developed epithelial tubules and delayed vascular development.
T1973	6862-6869	RB	denotes	However
T1975	6869-6871	,	denotes	,
T1976	6871-6875	NN	denotes	lung
T1978	6876-6884	JJ	denotes	saccular
T1977	6885-6896	NN	denotes	development
T1979	6897-6900	VBD	denotes	was
T1974	6901-6909	VBN	denotes	impaired
T1980	6909-6911	,	denotes	,
T1981	6911-6915	IN	denotes	with
T1982	6916-6924	VBN	denotes	arrested
T1984	6925-6927	CC	denotes	or
T1985	6928-6937	RB	denotes	partially
T1986	6938-6947	VBN	denotes	developed
T1987	6948-6958	JJ	denotes	epithelial
T1983	6959-6966	NNS	denotes	tubules
T1988	6967-6970	CC	denotes	and
T1989	6971-6978	VBN	denotes	delayed
T1991	6979-6987	JJ	denotes	vascular
T1990	6988-6999	NN	denotes	development
T1992	6999-7000	.	denotes	.
T1993	7000-7134	sentence	denotes	Differentiation of type II alveolar epithelial cells (AECs) appeared normal but differentiation of type I AECs was severely impaired.
T1994	7001-7016	NN	denotes	Differentiation
T1996	7017-7019	IN	denotes	of
T1997	7020-7024	NN	denotes	type
T1998	7025-7027	CD	denotes	II
T1999	7028-7036	JJ	denotes	alveolar
T2001	7037-7047	JJ	denotes	epithelial
T2000	7048-7053	NNS	denotes	cells
T2002	7054-7055	-LRB-	denotes	(
T2003	7055-7059	NNS	denotes	AECs
T2004	7059-7060	-RRB-	denotes	)
T1995	7061-7069	VBD	denotes	appeared
T2005	7070-7076	JJ	denotes	normal
T2006	7077-7080	CC	denotes	but
T2007	7081-7096	NN	denotes	differentiation
T2009	7097-7099	IN	denotes	of
T2010	7100-7104	NN	denotes	type
T2012	7105-7106	CD	denotes	I
T2011	7107-7111	NNS	denotes	AECs
T2013	7112-7115	VBD	denotes	was
T2014	7116-7124	RB	denotes	severely
T2008	7125-7133	VBN	denotes	impaired
T2015	7133-7134	.	denotes	.
T2016	7134-7339	sentence	denotes	Therefore, cholesterol deficiency resulted in a distinct late gestational lung hypoplasia, characterized by impaired sacculation with delayed type I AECs differentiation and immature vascular development.
T2017	7135-7144	RB	denotes	Therefore
T2019	7144-7146	,	denotes	,
T2020	7146-7157	NN	denotes	cholesterol
T2021	7158-7168	NN	denotes	deficiency
T2018	7169-7177	VBD	denotes	resulted
T2022	7178-7180	IN	denotes	in
T2023	7181-7182	DT	denotes	a
T2025	7183-7191	JJ	denotes	distinct
T2026	7192-7196	JJ	denotes	late
T2027	7197-7208	JJ	denotes	gestational
T2028	7209-7213	NN	denotes	lung
T2024	7214-7224	NN	denotes	hypoplasia
T2029	7224-7226	,	denotes	,
T2030	7226-7239	VBN	denotes	characterized
T2031	7240-7242	IN	denotes	by
T2032	7243-7251	JJ	denotes	impaired
T2033	7252-7263	NN	denotes	sacculation
T2034	7264-7268	IN	denotes	with
T2035	7269-7276	VBN	denotes	delayed
T2037	7277-7281	NN	denotes	type
T2039	7282-7283	CD	denotes	I
T2038	7284-7288	NNS	denotes	AECs
T2036	7289-7304	NN	denotes	differentiation
T2040	7305-7308	CC	denotes	and
T2041	7309-7317	JJ	denotes	immature
T2043	7318-7326	JJ	denotes	vascular
T2042	7327-7338	NN	denotes	development
T2044	7338-7339	.	denotes	.
T2045	7339-7524	sentence	denotes	However, this study does not support the concept that the perturbation of Shh-Patched-Gli pathway was involved in the pathogenesis of developmental lung hypoplasia in Dhcr7-/- embryos.
T2046	7340-7347	RB	denotes	However
T2048	7347-7349	,	denotes	,
T2049	7349-7353	DT	denotes	this
T2050	7354-7359	NN	denotes	study
T2051	7360-7364	VBZ	denotes	does
T2052	7365-7368	RB	denotes	not
T2047	7369-7376	VB	denotes	support
T2053	7377-7380	DT	denotes	the
T2054	7381-7388	NN	denotes	concept
T2055	7389-7393	IN	denotes	that
T2057	7394-7397	DT	denotes	the
T2058	7398-7410	NN	denotes	perturbation
T2059	7411-7413	IN	denotes	of
T2060	7414-7417	NN	denotes	Shh
T2062	7417-7418	HYPH	denotes	-
T2061	7418-7425	VBN	denotes	Patched
T2064	7425-7426	HYPH	denotes	-
T2063	7426-7429	NN	denotes	Gli
T2065	7430-7437	NN	denotes	pathway
T2066	7438-7441	VBD	denotes	was
T2056	7442-7450	VBN	denotes	involved
T2067	7451-7453	IN	denotes	in
T2068	7454-7457	DT	denotes	the
T2069	7458-7470	NN	denotes	pathogenesis
T2070	7471-7473	IN	denotes	of
T2071	7474-7487	JJ	denotes	developmental
T2073	7488-7492	NN	denotes	lung
T2072	7493-7503	NN	denotes	hypoplasia
T2074	7504-7506	IN	denotes	in
T2075	7507-7512	NN	denotes	Dhcr7
T2077	7512-7513	SYM	denotes	-
T2078	7513-7514	SYM	denotes	/
T2079	7514-7515	SYM	denotes	-
T2076	7516-7523	NNS	denotes	embryos
T2080	7523-7524	.	denotes	.
T2081	7524-7637	sentence	denotes	Patterns of expression of Shh signaling cascade members were not altered, and processing of Shh appeared normal.
T2082	7525-7533	NNS	denotes	Patterns
T2084	7534-7536	IN	denotes	of
T2085	7537-7547	NN	denotes	expression
T2086	7548-7550	IN	denotes	of
T2087	7551-7554	NN	denotes	Shh
T2089	7555-7564	NN	denotes	signaling
T2088	7565-7572	NN	denotes	cascade
T2090	7573-7580	NNS	denotes	members
T2091	7581-7585	VBD	denotes	were
T2092	7586-7589	RB	denotes	not
T2083	7590-7597	VBN	denotes	altered
T2093	7597-7599	,	denotes	,
T2094	7599-7602	CC	denotes	and
T2095	7603-7613	NN	denotes	processing
T2097	7614-7616	IN	denotes	of
T2098	7617-7620	NN	denotes	Shh
T2096	7621-7629	VBD	denotes	appeared
T2099	7630-7636	JJ	denotes	normal
T2100	7636-7637	.	denotes	.
T2191	7648-7652	NN	denotes	Body
T2192	7653-7659	NN	denotes	growth
T2193	7660-7671	NN	denotes	retardation
T2194	7672-7674	IN	denotes	at
T2195	7675-7679	JJ	denotes	late
T2197	7680-7689	NN	denotes	gestation
T2196	7690-7695	NN	denotes	stage
T2198	7696-7698	IN	denotes	in
T2199	7699-7704	NN	denotes	Dhcr7
T2201	7704-7705	SYM	denotes	-
T2202	7705-7706	SYM	denotes	/
T2203	7706-7707	SYM	denotes	-
T2200	7708-7715	NNS	denotes	embryos
T2204	7715-7874	sentence	denotes	Dhcr7-/- mice were born in the expected Mendelian ratios from heterozygous parents, but all of these pups died within 24 h and most within 14 h of birth [16].
T2205	7716-7721	NN	denotes	Dhcr7
T2207	7721-7722	SYM	denotes	-
T2208	7722-7723	SYM	denotes	/
T2209	7723-7724	SYM	denotes	-
T2206	7725-7729	NNS	denotes	mice
T2211	7730-7734	VBD	denotes	were
T2210	7735-7739	VBN	denotes	born
T2212	7740-7742	IN	denotes	in
T2213	7743-7746	DT	denotes	the
T2215	7747-7755	VBN	denotes	expected
T2216	7756-7765	JJ	denotes	Mendelian
T2214	7766-7772	NNS	denotes	ratios
T2217	7773-7777	IN	denotes	from
T2218	7778-7790	JJ	denotes	heterozygous
T2219	7791-7798	NNS	denotes	parents
T2220	7798-7800	,	denotes	,
T2221	7800-7803	CC	denotes	but
T2222	7804-7807	DT	denotes	all
T2224	7808-7810	IN	denotes	of
T2225	7811-7816	DT	denotes	these
T2226	7817-7821	NNS	denotes	pups
T2223	7822-7826	VBD	denotes	died
T2227	7827-7833	IN	denotes	within
T2228	7834-7836	CD	denotes	24
T2229	7837-7838	NN	denotes	h
T2230	7839-7842	CC	denotes	and
T2231	7843-7847	JJS	denotes	most
T2232	7848-7854	IN	denotes	within
T2233	7855-7857	CD	denotes	14
T2234	7858-7859	NN	denotes	h
T2235	7860-7862	IN	denotes	of
T2236	7863-7868	NN	denotes	birth
T2237	7869-7870	-LRB-	denotes	[
T2238	7870-7872	CD	denotes	16
T2239	7872-7873	-RRB-	denotes	]
T2240	7873-7874	.	denotes	.
T2241	7874-7954	sentence	denotes	At birth, they were clearly smaller than wild-type or heterozygous littermates.
T2242	7875-7877	IN	denotes	At
T2244	7878-7883	NN	denotes	birth
T2245	7883-7885	,	denotes	,
T2246	7885-7889	PRP	denotes	they
T2243	7890-7894	VBD	denotes	were
T2247	7895-7902	RB	denotes	clearly
T2248	7903-7910	JJR	denotes	smaller
T2249	7911-7915	IN	denotes	than
T2250	7916-7920	JJ	denotes	wild
T2252	7920-7921	HYPH	denotes	-
T2251	7921-7925	NN	denotes	type
T2254	7926-7928	CC	denotes	or
T2255	7929-7941	JJ	denotes	heterozygous
T2253	7942-7953	NNS	denotes	littermates
T2256	7953-7954	.	denotes	.
T2257	7954-8094	sentence	denotes	An experienced investigator can pick out knockout pups based on this along with presence of hypoxia and their lack of spontaneous movement.
T2258	7955-7957	DT	denotes	An
T2260	7958-7969	JJ	denotes	experienced
T2259	7970-7982	NN	denotes	investigator
T2262	7983-7986	MD	denotes	can
T2261	7987-7991	VB	denotes	pick
T2263	7992-7995	RP	denotes	out
T2264	7996-8004	NN	denotes	knockout
T2265	8005-8009	NNS	denotes	pups
T2266	8010-8015	VBN	denotes	based
T2267	8016-8018	IN	denotes	on
T2268	8019-8023	DT	denotes	this
T2269	8024-8029	IN	denotes	along
T2270	8030-8034	IN	denotes	with
T2271	8035-8043	NN	denotes	presence
T2272	8044-8046	IN	denotes	of
T2273	8047-8054	NN	denotes	hypoxia
T2274	8055-8058	CC	denotes	and
T2275	8059-8064	PRP$	denotes	their
T2276	8065-8069	NN	denotes	lack
T2277	8070-8072	IN	denotes	of
T2278	8073-8084	JJ	denotes	spontaneous
T2279	8085-8093	NN	denotes	movement
T2280	8093-8094	.	denotes	.
T2281	8094-8372	sentence	denotes	The breathing frequency in P0 Dhcr7-/- pups was markedly slower than control littermates (34 ± 7 breaths/min in Dhcr7-/-, n = 10 vs. 92 ± 16 breaths/min in controls, n = 32, p < 0.001) and the breathing was observed to be more irregular and shallow, with long periods of apnea.
T2282	8095-8098	DT	denotes	The
T2284	8099-8108	NN	denotes	breathing
T2283	8109-8118	NN	denotes	frequency
T2286	8119-8121	IN	denotes	in
T2287	8122-8124	NN	denotes	P0
T2289	8125-8130	NN	denotes	Dhcr7
T2290	8130-8131	SYM	denotes	-
T2291	8131-8132	SYM	denotes	/
T2292	8132-8133	SYM	denotes	-
T2288	8134-8138	NNS	denotes	pups
T2285	8139-8142	VBD	denotes	was
T2293	8143-8151	RB	denotes	markedly
T2294	8152-8158	JJR	denotes	slower
T2295	8159-8163	IN	denotes	than
T2296	8164-8171	NN	denotes	control
T2297	8172-8183	NNS	denotes	littermates
T2298	8184-8185	-LRB-	denotes	(
T2300	8185-8187	CD	denotes	34
T2302	8188-8189	SYM	denotes	±
T2301	8190-8191	CD	denotes	7
T2299	8192-8199	NNS	denotes	breaths
T2303	8199-8200	SYM	denotes	/
T2304	8200-8203	NN	denotes	min
T2305	8204-8206	IN	denotes	in
T2306	8207-8212	NN	denotes	Dhcr7
T2307	8212-8213	SYM	denotes	-
T2308	8213-8214	SYM	denotes	/
T2309	8214-8215	SYM	denotes	-
T2310	8215-8217	,	denotes	,
T2311	8217-8218	NN	denotes	n
T2313	8219-8220	SYM	denotes	=
T2314	8221-8223	CD	denotes	10
T2315	8224-8227	CC	denotes	vs.
T2316	8228-8230	CD	denotes	92
T2318	8231-8232	SYM	denotes	±
T2317	8233-8235	CD	denotes	16
T2312	8236-8243	NNS	denotes	breaths
T2319	8243-8244	SYM	denotes	/
T2320	8244-8247	NN	denotes	min
T2321	8248-8250	IN	denotes	in
T2322	8251-8259	NNS	denotes	controls
T2323	8259-8261	,	denotes	,
T2324	8261-8262	NN	denotes	n
T2326	8263-8264	SYM	denotes	=
T2325	8265-8267	CD	denotes	32
T2327	8267-8269	,	denotes	,
T2328	8269-8270	NN	denotes	p
T2330	8271-8272	SYM	denotes	<
T2329	8273-8278	CD	denotes	0.001
T2331	8278-8279	-RRB-	denotes	)
T2332	8280-8283	CC	denotes	and
T2333	8284-8287	DT	denotes	the
T2334	8288-8297	NN	denotes	breathing
T2336	8298-8301	VBD	denotes	was
T2335	8302-8310	VBN	denotes	observed
T2337	8311-8313	TO	denotes	to
T2338	8314-8316	VB	denotes	be
T2339	8317-8321	RBR	denotes	more
T2340	8322-8331	JJ	denotes	irregular
T2341	8332-8335	CC	denotes	and
T2342	8336-8343	JJ	denotes	shallow
T2343	8343-8345	,	denotes	,
T2344	8345-8349	IN	denotes	with
T2345	8350-8354	JJ	denotes	long
T2346	8355-8362	NNS	denotes	periods
T2347	8363-8365	IN	denotes	of
T2348	8366-8371	NN	denotes	apnea
T2349	8371-8372	.	denotes	.
T2350	8372-8578	sentence	denotes	To determine the gestational stage at which growth of the knockout embryos was affected, embryos were harvested at various stages of gestation and their body lengths compared between Dhcr7-/- and controls.
T2351	8373-8375	TO	denotes	To
T2352	8376-8385	VB	denotes	determine
T2354	8386-8389	DT	denotes	the
T2356	8390-8401	JJ	denotes	gestational
T2355	8402-8407	NN	denotes	stage
T2357	8408-8410	IN	denotes	at
T2359	8411-8416	WDT	denotes	which
T2360	8417-8423	NN	denotes	growth
T2361	8424-8426	IN	denotes	of
T2362	8427-8430	DT	denotes	the
T2364	8431-8439	NN	denotes	knockout
T2363	8440-8447	NNS	denotes	embryos
T2365	8448-8451	VBD	denotes	was
T2358	8452-8460	VBN	denotes	affected
T2366	8460-8462	,	denotes	,
T2367	8462-8469	NNS	denotes	embryos
T2368	8470-8474	VBD	denotes	were
T2353	8475-8484	VBN	denotes	harvested
T2369	8485-8487	IN	denotes	at
T2370	8488-8495	JJ	denotes	various
T2371	8496-8502	NNS	denotes	stages
T2372	8503-8505	IN	denotes	of
T2373	8506-8515	NN	denotes	gestation
T2374	8516-8519	CC	denotes	and
T2375	8520-8525	PRP$	denotes	their
T2377	8526-8530	NN	denotes	body
T2376	8531-8538	NNS	denotes	lengths
T2378	8539-8547	VBN	denotes	compared
T2379	8548-8555	IN	denotes	between
T2380	8556-8561	NN	denotes	Dhcr7
T2381	8561-8562	SYM	denotes	-
T2382	8562-8563	SYM	denotes	/
T2383	8563-8564	SYM	denotes	-
T2384	8565-8568	CC	denotes	and
T2385	8569-8577	NNS	denotes	controls
T2386	8577-8578	.	denotes	.
T2387	8578-8672	sentence	denotes	A linear increase in body length from E9.5 to E19.5 in control embryos was observed (Fig. 1).
T2388	8579-8580	DT	denotes	A
T2390	8581-8587	JJ	denotes	linear
T2389	8588-8596	NN	denotes	increase
T2392	8597-8599	IN	denotes	in
T2393	8600-8604	NN	denotes	body
T2394	8605-8611	NN	denotes	length
T2395	8612-8616	IN	denotes	from
T2396	8617-8621	NN	denotes	E9.5
T2397	8622-8624	IN	denotes	to
T2398	8625-8630	NN	denotes	E19.5
T2399	8631-8633	IN	denotes	in
T2400	8634-8641	NN	denotes	control
T2401	8642-8649	NNS	denotes	embryos
T2402	8650-8653	VBD	denotes	was
T2391	8654-8662	VBN	denotes	observed
T2403	8663-8664	-LRB-	denotes	(
T2404	8664-8668	NN	denotes	Fig.
T2405	8669-8670	CD	denotes	1
T2406	8670-8671	-RRB-	denotes	)
T2407	8671-8672	.	denotes	.
T2408	8672-8786	sentence	denotes	No significant differences in body length between wild-type and heterozygous embryos were noted (data not shown).
T2409	8673-8675	DT	denotes	No
T2411	8676-8687	JJ	denotes	significant
T2410	8688-8699	NNS	denotes	differences
T2413	8700-8702	IN	denotes	in
T2414	8703-8707	NN	denotes	body
T2415	8708-8714	NN	denotes	length
T2416	8715-8722	IN	denotes	between
T2417	8723-8727	JJ	denotes	wild
T2419	8727-8728	HYPH	denotes	-
T2418	8728-8732	NN	denotes	type
T2421	8733-8736	CC	denotes	and
T2422	8737-8749	JJ	denotes	heterozygous
T2420	8750-8757	NNS	denotes	embryos
T2423	8758-8762	VBD	denotes	were
T2412	8763-8768	VBN	denotes	noted
T2424	8769-8770	-LRB-	denotes	(
T2426	8770-8774	NNS	denotes	data
T2427	8775-8778	RB	denotes	not
T2425	8779-8784	VBN	denotes	shown
T2428	8784-8785	-RRB-	denotes	)
T2429	8785-8786	.	denotes	.
T2430	8786-9079	sentence	denotes	In contrast, knockout embryos showed comparable body length growth until E14.5~E16.5, then began to show a slower increase in length, such that by E17.5, this was statistically significant (18.87 ± 1.29 mm in controls, n = 15 compared to 16.69 ± 1.05 mm in Dhcr7-/-, n = 7, p < 0.05, Fig. 1).
T2431	8787-8789	IN	denotes	In
T2433	8790-8798	NN	denotes	contrast
T2434	8798-8800	,	denotes	,
T2435	8800-8808	NN	denotes	knockout
T2436	8809-8816	NNS	denotes	embryos
T2432	8817-8823	VBD	denotes	showed
T2437	8824-8834	JJ	denotes	comparable
T2439	8835-8839	NN	denotes	body
T2440	8840-8846	NN	denotes	length
T2438	8847-8853	NN	denotes	growth
T2441	8854-8859	IN	denotes	until
T2442	8860-8865	NN	denotes	E14.5
T2443	8865-8866	SYM	denotes	~
T2444	8866-8871	NN	denotes	E16.5
T2445	8871-8873	,	denotes	,
T2446	8873-8877	RB	denotes	then
T2447	8878-8883	VBD	denotes	began
T2448	8884-8886	TO	denotes	to
T2449	8887-8891	VB	denotes	show
T2450	8892-8893	DT	denotes	a
T2452	8894-8900	JJR	denotes	slower
T2451	8901-8909	NN	denotes	increase
T2453	8910-8912	IN	denotes	in
T2454	8913-8919	NN	denotes	length
T2455	8919-8921	,	denotes	,
T2456	8921-8925	JJ	denotes	such
T2458	8926-8930	IN	denotes	that
T2459	8931-8933	IN	denotes	by
T2460	8934-8939	NN	denotes	E17.5
T2461	8939-8941	,	denotes	,
T2462	8941-8945	DT	denotes	this
T2457	8946-8949	VBD	denotes	was
T2463	8950-8963	RB	denotes	statistically
T2464	8964-8975	JJ	denotes	significant
T2465	8976-8977	-LRB-	denotes	(
T2467	8977-8982	CD	denotes	18.87
T2469	8983-8984	SYM	denotes	±
T2468	8985-8989	CD	denotes	1.29
T2470	8990-8992	NNS	denotes	mm
T2471	8993-8995	IN	denotes	in
T2472	8996-9004	NNS	denotes	controls
T2473	9004-9006	,	denotes	,
T2474	9006-9007	NN	denotes	n
T2476	9008-9009	SYM	denotes	=
T2475	9010-9012	CD	denotes	15
T2477	9013-9021	VBN	denotes	compared
T2478	9022-9024	IN	denotes	to
T2479	9025-9030	CD	denotes	16.69
T2481	9031-9032	SYM	denotes	±
T2480	9033-9037	CD	denotes	1.05
T2482	9038-9040	NNS	denotes	mm
T2483	9041-9043	IN	denotes	in
T2484	9044-9049	NN	denotes	Dhcr7
T2485	9049-9050	SYM	denotes	-
T2486	9050-9051	SYM	denotes	/
T2487	9051-9052	SYM	denotes	-
T2488	9052-9054	,	denotes	,
T2489	9054-9055	NN	denotes	n
T2491	9056-9057	SYM	denotes	=
T2490	9058-9059	CD	denotes	7
T2492	9059-9061	,	denotes	,
T2493	9061-9062	NN	denotes	p
T2495	9063-9064	SYM	denotes	<
T2494	9065-9069	CD	denotes	0.05
T2496	9069-9071	,	denotes	,
T2466	9071-9075	NN	denotes	Fig.
T2497	9076-9077	CD	denotes	1
T2498	9077-9078	-RRB-	denotes	)
T2499	9078-9079	.	denotes	.
T11053	9090-9094	JJ	denotes	Body
T11054	9095-9101	NN	denotes	length
T11056	9102-9108	NN	denotes	growth
T11055	9109-9115	NNS	denotes	curves
T11057	9115-9116	.	denotes	.
T11058	9116-9169	sentence	denotes	Body length growth vs. gestational days is as shown.
T11059	9117-9121	JJ	denotes	Body
T11060	9122-9128	NN	denotes	length
T11061	9129-9135	NN	denotes	growth
T11063	9136-9139	CC	denotes	vs.
T11064	9140-9151	JJ	denotes	gestational
T11065	9152-9156	NNS	denotes	days
T11062	9157-9159	VBZ	denotes	is
T11066	9160-9162	IN	denotes	as
T11067	9163-9168	VBN	denotes	shown
T11068	9168-9169	.	denotes	.
T11069	9169-9368	sentence	denotes	Control embryos (filled triangles, wild-type and heterozygous embryos were pooled together as there were no differences between these two groups) and knockout embryos (filled circles) were as shown.
T11070	9170-9177	NN	denotes	Control
T11071	9178-9185	NNS	denotes	embryos
T11073	9186-9187	-LRB-	denotes	(
T11075	9187-9193	VBN	denotes	filled
T11074	9194-9203	NNS	denotes	triangles
T11076	9203-9205	,	denotes	,
T11077	9205-9209	JJ	denotes	wild
T11079	9209-9210	HYPH	denotes	-
T11078	9210-9214	NN	denotes	type
T11081	9215-9218	CC	denotes	and
T11082	9219-9231	JJ	denotes	heterozygous
T11080	9232-9239	NNS	denotes	embryos
T11084	9240-9244	VBD	denotes	were
T11083	9245-9251	VBN	denotes	pooled
T11085	9252-9260	RB	denotes	together
T11086	9261-9263	IN	denotes	as
T11088	9264-9269	EX	denotes	there
T11087	9270-9274	VBD	denotes	were
T11089	9275-9277	DT	denotes	no
T11090	9278-9289	NNS	denotes	differences
T11091	9290-9297	IN	denotes	between
T11092	9298-9303	DT	denotes	these
T11094	9304-9307	CD	denotes	two
T11093	9308-9314	NNS	denotes	groups
T11095	9314-9315	-RRB-	denotes	)
T11096	9316-9319	CC	denotes	and
T11097	9320-9328	NN	denotes	knockout
T11098	9329-9336	NNS	denotes	embryos
T11099	9337-9338	-LRB-	denotes	(
T11101	9338-9344	VBN	denotes	filled
T11100	9345-9352	NNS	denotes	circles
T11102	9352-9353	-RRB-	denotes	)
T11072	9354-9358	VBD	denotes	were
T11103	9359-9361	IN	denotes	as
T11104	9362-9367	VBN	denotes	shown
T11105	9367-9368	.	denotes	.
T11106	9368-9486	sentence	denotes	The growth curve showed a linear increase from E9.5 to E19.5 in controls, but deviated at ~E14.5 in Dhcr7-/- embryos.
T11107	9369-9372	DT	denotes	The
T11109	9373-9379	NN	denotes	growth
T11108	9380-9385	NN	denotes	curve
T11110	9386-9392	VBD	denotes	showed
T11111	9393-9394	DT	denotes	a
T11113	9395-9401	JJ	denotes	linear
T11112	9402-9410	NN	denotes	increase
T11114	9411-9415	IN	denotes	from
T11115	9416-9420	NN	denotes	E9.5
T11116	9421-9423	IN	denotes	to
T11117	9424-9429	NN	denotes	E19.5
T11118	9430-9432	IN	denotes	in
T11119	9433-9441	NNS	denotes	controls
T11120	9441-9443	,	denotes	,
T11121	9443-9446	CC	denotes	but
T11122	9447-9455	VBN	denotes	deviated
T11123	9456-9458	IN	denotes	at
T11124	9459-9460	SYM	denotes	~
T11125	9460-9465	NN	denotes	E14.5
T11126	9466-9468	IN	denotes	in
T11127	9469-9474	NN	denotes	Dhcr7
T11129	9474-9475	SYM	denotes	-
T11130	9475-9476	SYM	denotes	/
T11131	9476-9477	SYM	denotes	-
T11128	9478-9485	NNS	denotes	embryos
T11132	9485-9486	.	denotes	.
T11133	9486-9844	sentence	denotes	Body lengths at E17.5 (18.87 ± 1.29 mm in controls, n = 15 vs. 16.69 ± 1.05 mm in Dhcr7-/-, n = 7, p < 0.05), E18.5 (21.35 ± 1.05 mm in controls, n = 15 vs. 19.38 ± 0.9 mm in Dhcr7-/-, n = 5, p < 0.05) and E19.5 (23.4 ± 0.97 mm in controls, n = 13 vs. 20.3 ± 0.44 mm in Dhcr7-/-, n = 4, p < 0.05) were significantly decreased, compared to those in controls.
T11134	9487-9491	NN	denotes	Body
T11135	9492-9499	NNS	denotes	lengths
T11137	9500-9502	IN	denotes	at
T11138	9503-9508	NN	denotes	E17.5
T11139	9509-9510	-LRB-	denotes	(
T11141	9510-9515	CD	denotes	18.87
T11143	9516-9517	SYM	denotes	±
T11142	9518-9522	CD	denotes	1.29
T11140	9523-9525	NNS	denotes	mm
T11144	9526-9528	IN	denotes	in
T11145	9529-9537	NNS	denotes	controls
T11146	9537-9539	,	denotes	,
T11147	9539-9540	NN	denotes	n
T11149	9541-9542	SYM	denotes	=
T11150	9543-9545	CD	denotes	15
T11151	9546-9549	CC	denotes	vs.
T11152	9550-9555	CD	denotes	16.69
T11154	9556-9557	SYM	denotes	±
T11153	9558-9562	CD	denotes	1.05
T11148	9563-9565	NNS	denotes	mm
T11155	9566-9568	IN	denotes	in
T11156	9569-9574	NN	denotes	Dhcr7
T11157	9574-9575	SYM	denotes	-
T11158	9575-9576	SYM	denotes	/
T11159	9576-9577	SYM	denotes	-
T11160	9577-9579	,	denotes	,
T11161	9579-9580	NN	denotes	n
T11163	9581-9582	SYM	denotes	=
T11162	9583-9584	CD	denotes	7
T11164	9584-9586	,	denotes	,
T11165	9586-9587	NN	denotes	p
T11167	9588-9589	SYM	denotes	<
T11166	9590-9594	CD	denotes	0.05
T11168	9594-9595	-RRB-	denotes	)
T11169	9595-9597	,	denotes	,
T11170	9597-9602	NN	denotes	E18.5
T11171	9603-9604	-LRB-	denotes	(
T11173	9604-9609	CD	denotes	21.35
T11175	9610-9611	SYM	denotes	±
T11174	9612-9616	CD	denotes	1.05
T11172	9617-9619	NNS	denotes	mm
T11176	9620-9622	IN	denotes	in
T11177	9623-9631	NNS	denotes	controls
T11178	9631-9633	,	denotes	,
T11179	9633-9634	NN	denotes	n
T11181	9635-9636	SYM	denotes	=
T11182	9637-9639	CD	denotes	15
T11183	9640-9643	CC	denotes	vs.
T11184	9644-9649	CD	denotes	19.38
T11186	9650-9651	SYM	denotes	±
T11185	9652-9655	CD	denotes	0.9
T11180	9656-9658	NNS	denotes	mm
T11187	9659-9661	IN	denotes	in
T11188	9662-9667	NN	denotes	Dhcr7
T11189	9667-9668	SYM	denotes	-
T11190	9668-9669	SYM	denotes	/
T11191	9669-9670	SYM	denotes	-
T11192	9670-9672	,	denotes	,
T11193	9672-9673	NN	denotes	n
T11195	9674-9675	SYM	denotes	=
T11194	9676-9677	CD	denotes	5
T11196	9677-9679	,	denotes	,
T11197	9679-9680	NN	denotes	p
T11199	9681-9682	SYM	denotes	<
T11198	9683-9687	CD	denotes	0.05
T11200	9687-9688	-RRB-	denotes	)
T11201	9689-9692	CC	denotes	and
T11202	9693-9698	NN	denotes	E19.5
T11203	9699-9700	-LRB-	denotes	(
T11205	9700-9704	CD	denotes	23.4
T11207	9705-9706	SYM	denotes	±
T11206	9707-9711	CD	denotes	0.97
T11204	9712-9714	NNS	denotes	mm
T11208	9715-9717	IN	denotes	in
T11209	9718-9726	NNS	denotes	controls
T11210	9726-9728	,	denotes	,
T11211	9728-9729	NN	denotes	n
T11213	9730-9731	SYM	denotes	=
T11214	9732-9734	CD	denotes	13
T11215	9735-9738	CC	denotes	vs.
T11216	9739-9743	CD	denotes	20.3
T11218	9744-9745	SYM	denotes	±
T11217	9746-9750	CD	denotes	0.44
T11212	9751-9753	NNS	denotes	mm
T11219	9754-9756	IN	denotes	in
T11220	9757-9762	NN	denotes	Dhcr7
T11221	9762-9763	SYM	denotes	-
T11222	9763-9764	SYM	denotes	/
T11223	9764-9765	SYM	denotes	-
T11224	9765-9767	,	denotes	,
T11225	9767-9768	NN	denotes	n
T11227	9769-9770	SYM	denotes	=
T11226	9771-9772	CD	denotes	4
T11228	9772-9774	,	denotes	,
T11229	9774-9775	NN	denotes	p
T11231	9776-9777	SYM	denotes	<
T11230	9778-9782	CD	denotes	0.05
T11232	9782-9783	-RRB-	denotes	)
T11233	9784-9788	VBD	denotes	were
T11234	9789-9802	RB	denotes	significantly
T11136	9803-9812	VBN	denotes	decreased
T11235	9812-9814	,	denotes	,
T11236	9814-9822	VBN	denotes	compared
T11237	9823-9825	IN	denotes	to
T11238	9826-9831	DT	denotes	those
T11239	9832-9834	IN	denotes	in
T11240	9835-9843	NNS	denotes	controls
T11241	9843-9844	.	denotes	.
T11242	9844-9881	sentence	denotes	Results are expressed as mean ± s.d.
T11243	9845-9852	NNS	denotes	Results
T11245	9853-9856	VBP	denotes	are
T11244	9857-9866	VBN	denotes	expressed
T11246	9867-9869	IN	denotes	as
T11247	9870-9874	NN	denotes	mean
T11249	9875-9876	SYM	denotes	±
T11248	9877-9880	NN	denotes	s.d
T11250	9880-9881	.	denotes	.
T2667	9883-9888	NN	denotes	Dhcr7
T2669	9888-9889	SYM	denotes	-
T2670	9889-9890	SYM	denotes	/
T2671	9890-9891	SYM	denotes	-
T2668	9892-9899	NNS	denotes	embryos
T2673	9900-9902	IN	denotes	at
T2674	9903-9907	JJ	denotes	late
T2675	9908-9914	NNS	denotes	stages
T2672	9915-9924	VBD	denotes	displayed
T2676	9925-9934	JJ	denotes	pulmonary
T2677	9935-9945	NN	denotes	hypoplasia
T2678	9946-9950	IN	denotes	with
T2679	9951-9958	VBN	denotes	delayed
T2681	9959-9967	JJ	denotes	saccular
T2680	9968-9979	NN	denotes	development
T2682	9979-10140	sentence	denotes	Wet lung weights, as well as lung to body weight ratios were significantly lower in Dhcr7-/- at E18.5 compared to those in wild-type but not at E13.5 (Table 1).
T2683	9980-9983	JJ	denotes	Wet
T2684	9984-9988	NN	denotes	lung
T2685	9989-9996	NNS	denotes	weights
T2687	9996-9998	,	denotes	,
T2688	9998-10000	RB	denotes	as
T2690	10001-10005	RB	denotes	well
T2689	10006-10008	IN	denotes	as
T2691	10009-10013	NN	denotes	lung
T2693	10014-10016	IN	denotes	to
T2694	10017-10021	NN	denotes	body
T2695	10022-10028	NN	denotes	weight
T2692	10029-10035	NNS	denotes	ratios
T2686	10036-10040	VBD	denotes	were
T2696	10041-10054	RB	denotes	significantly
T2697	10055-10060	JJR	denotes	lower
T2698	10061-10063	IN	denotes	in
T2699	10064-10069	NN	denotes	Dhcr7
T2700	10069-10070	SYM	denotes	-
T2701	10070-10071	SYM	denotes	/
T2702	10071-10072	SYM	denotes	-
T2703	10073-10075	IN	denotes	at
T2704	10076-10081	NN	denotes	E18.5
T2705	10082-10090	VBN	denotes	compared
T2706	10091-10093	IN	denotes	to
T2707	10094-10099	DT	denotes	those
T2708	10100-10102	IN	denotes	in
T2709	10103-10107	JJ	denotes	wild
T2711	10107-10108	HYPH	denotes	-
T2710	10108-10112	NN	denotes	type
T2712	10113-10116	CC	denotes	but
T2713	10117-10120	RB	denotes	not
T2714	10121-10123	IN	denotes	at
T2715	10124-10129	NN	denotes	E13.5
T2716	10130-10131	-LRB-	denotes	(
T2717	10131-10136	NN	denotes	Table
T2718	10137-10138	CD	denotes	1
T2719	10138-10139	-RRB-	denotes	)
T2720	10139-10140	.	denotes	.
T2721	10140-10219	sentence	denotes	Both body and lung weights were reduced, but lungs seemed to be more affected.
T2722	10141-10145	CC	denotes	Both
T2723	10146-10150	NN	denotes	body
T2725	10151-10154	CC	denotes	and
T2726	10155-10159	NN	denotes	lung
T2724	10160-10167	NNS	denotes	weights
T2728	10168-10172	VBD	denotes	were
T2727	10173-10180	VBN	denotes	reduced
T2729	10180-10182	,	denotes	,
T2730	10182-10185	CC	denotes	but
T2731	10186-10191	NNS	denotes	lungs
T2732	10192-10198	VBD	denotes	seemed
T2733	10199-10201	TO	denotes	to
T2734	10202-10204	VB	denotes	be
T2735	10205-10209	RBR	denotes	more
T2736	10210-10218	JJ	denotes	affected
T2737	10218-10219	.	denotes	.
T2738	10219-10317	sentence	denotes	A representative figure of embryos at E10.5 and of the pups at birth was shown in Fig. 2A and 2B.
T2739	10220-10221	DT	denotes	A
T2741	10222-10236	JJ	denotes	representative
T2740	10237-10243	NN	denotes	figure
T2743	10244-10246	IN	denotes	of
T2744	10247-10254	NNS	denotes	embryos
T2745	10255-10257	IN	denotes	at
T2746	10258-10263	NN	denotes	E10.5
T2747	10264-10267	CC	denotes	and
T2748	10268-10270	IN	denotes	of
T2749	10271-10274	DT	denotes	the
T2750	10275-10279	NNS	denotes	pups
T2751	10280-10282	IN	denotes	at
T2752	10283-10288	NN	denotes	birth
T2753	10289-10292	VBD	denotes	was
T2742	10293-10298	VBN	denotes	shown
T2754	10299-10301	IN	denotes	in
T2755	10302-10306	NN	denotes	Fig.
T2756	10307-10309	CD	denotes	2A
T2757	10310-10313	CC	denotes	and
T2758	10314-10316	CD	denotes	2B
T2759	10316-10317	.	denotes	.
T2760	10317-10318	sentence	denotes
T14428	10327-10332	JJ	denotes	Whole
T14429	10333-10337	NN	denotes	body
T14431	10338-10341	CC	denotes	and
T14432	10342-10346	NN	denotes	lung
T14430	10347-10354	NNS	denotes	weights
T14433	10355-10357	IN	denotes	of
T14434	10358-10363	NN	denotes	mouse
T14435	10364-10371	NNS	denotes	embryos
T11316	10723-10732	JJ	denotes	Embryonic
T11317	10733-10737	NN	denotes	size
T11318	10737-10739	,	denotes	,
T11319	10739-10743	NN	denotes	lung
T11320	10744-10754	NN	denotes	morphology
T11321	10755-10758	CC	denotes	and
T11322	10759-10768	NN	denotes	histology
T11323	10769-10771	IN	denotes	at
T11324	10772-10779	JJ	denotes	various
T11325	10780-10786	NNS	denotes	stages
T11326	10787-10789	IN	denotes	of
T11327	10790-10801	NN	denotes	development
T11328	10801-10802	.	denotes	.
T11329	10802-10936	sentence	denotes	Panel A shows the size and general morphology of wild-type (+/+) and Dhcr7-/- (-/-) embryos at E10.5, without significant difference.
T11330	10803-10808	NN	denotes	Panel
T11331	10809-10810	NN	denotes	A
T11332	10811-10816	VBZ	denotes	shows
T11333	10817-10820	DT	denotes	the
T11334	10821-10825	NN	denotes	size
T11335	10826-10829	CC	denotes	and
T11336	10830-10837	JJ	denotes	general
T11337	10838-10848	NN	denotes	morphology
T11338	10849-10851	IN	denotes	of
T11339	10852-10856	JJ	denotes	wild
T11341	10856-10857	HYPH	denotes	-
T11340	10857-10861	NN	denotes	type
T11343	10862-10863	-LRB-	denotes	(
T11344	10863-10864	SYM	denotes	+
T11346	10864-10865	SYM	denotes	/
T11345	10865-10866	SYM	denotes	+
T11347	10866-10867	-RRB-	denotes	)
T11348	10868-10871	CC	denotes	and
T11349	10872-10877	NN	denotes	Dhcr7
T11350	10877-10878	SYM	denotes	-
T11351	10878-10879	SYM	denotes	/
T11352	10879-10880	SYM	denotes	-
T11353	10881-10882	-LRB-	denotes	(
T11354	10882-10883	SYM	denotes	-
T11356	10883-10884	SYM	denotes	/
T11355	10884-10885	SYM	denotes	-
T11357	10885-10886	-RRB-	denotes	)
T11342	10887-10894	NNS	denotes	embryos
T11358	10895-10897	IN	denotes	at
T11359	10898-10903	NN	denotes	E10.5
T11360	10903-10905	,	denotes	,
T11361	10905-10912	IN	denotes	without
T11362	10913-10924	JJ	denotes	significant
T11363	10925-10935	NN	denotes	difference
T11364	10935-10936	.	denotes	.
T11365	10936-11020	sentence	denotes	At P0, the knockout pups were significantly smaller and appeared hypoxic (panel B).
T11366	10937-10939	IN	denotes	At
T11368	10940-10942	NN	denotes	P0
T11369	10942-10944	,	denotes	,
T11370	10944-10947	DT	denotes	the
T11372	10948-10956	NN	denotes	knockout
T11371	10957-10961	NNS	denotes	pups
T11367	10962-10966	VBD	denotes	were
T11373	10967-10980	RB	denotes	significantly
T11374	10981-10988	JJR	denotes	smaller
T11375	10989-10992	CC	denotes	and
T11376	10993-11001	VBD	denotes	appeared
T11377	11002-11009	JJ	denotes	hypoxic
T11378	11010-11011	-LRB-	denotes	(
T11380	11011-11016	NN	denotes	panel
T11379	11017-11018	NN	denotes	B
T11381	11018-11019	-RRB-	denotes	)
T11382	11019-11020	.	denotes	.
T11383	11020-11118	sentence	denotes	Panel C shows dorsal views of lungs from knockout and wild-type at E13.5 and panel D those at P0.
T11384	11021-11026	NN	denotes	Panel
T11385	11027-11028	NN	denotes	C
T11386	11029-11034	VBZ	denotes	shows
T11387	11035-11041	JJ	denotes	dorsal
T11388	11042-11047	NNS	denotes	views
T11389	11048-11050	IN	denotes	of
T11390	11051-11056	NNS	denotes	lungs
T11391	11057-11061	IN	denotes	from
T11392	11062-11070	NN	denotes	knockout
T11393	11071-11074	CC	denotes	and
T11394	11075-11079	JJ	denotes	wild
T11396	11079-11080	HYPH	denotes	-
T11395	11080-11084	NN	denotes	type
T11397	11085-11087	IN	denotes	at
T11398	11088-11093	NN	denotes	E13.5
T11399	11094-11097	CC	denotes	and
T11400	11098-11103	NN	denotes	panel
T11401	11104-11105	NN	denotes	D
T11402	11106-11111	DT	denotes	those
T11403	11112-11114	IN	denotes	at
T11404	11115-11117	NN	denotes	P0
T11405	11117-11118	.	denotes	.
T11406	11118-11338	sentence	denotes	Cross-sectional examination at the cardiopulmonary level at E 20.5 (prior to birth) showed normal lobar septation, but lungs from knockout animals were smaller in size and had less distal sac space (cf. panels E and F).
T11407	11119-11124	JJ	denotes	Cross
T11409	11124-11125	HYPH	denotes	-
T11408	11125-11134	JJ	denotes	sectional
T11410	11135-11146	NN	denotes	examination
T11412	11147-11149	IN	denotes	at
T11413	11150-11153	DT	denotes	the
T11415	11154-11169	JJ	denotes	cardiopulmonary
T11414	11170-11175	NN	denotes	level
T11416	11176-11178	IN	denotes	at
T11417	11179-11180	NN	denotes	E
T11418	11181-11185	CD	denotes	20.5
T11419	11186-11187	-LRB-	denotes	(
T11421	11187-11192	JJ	denotes	prior
T11420	11193-11195	IN	denotes	to
T11422	11196-11201	NN	denotes	birth
T11423	11201-11202	-RRB-	denotes	)
T11411	11203-11209	VBD	denotes	showed
T11424	11210-11216	JJ	denotes	normal
T11426	11217-11222	NN	denotes	lobar
T11425	11223-11232	NN	denotes	septation
T11427	11232-11234	,	denotes	,
T11428	11234-11237	CC	denotes	but
T11429	11238-11243	NNS	denotes	lungs
T11431	11244-11248	IN	denotes	from
T11432	11249-11257	NN	denotes	knockout
T11433	11258-11265	NNS	denotes	animals
T11430	11266-11270	VBD	denotes	were
T11434	11271-11278	JJR	denotes	smaller
T11435	11279-11281	IN	denotes	in
T11436	11282-11286	NN	denotes	size
T11437	11287-11290	CC	denotes	and
T11438	11291-11294	VBD	denotes	had
T11439	11295-11299	JJR	denotes	less
T11440	11300-11306	JJ	denotes	distal
T11442	11307-11310	NN	denotes	sac
T11441	11311-11316	NN	denotes	space
T11443	11317-11318	-LRB-	denotes	(
T11445	11318-11321	RB	denotes	cf.
T11446	11322-11328	NNS	denotes	panels
T11444	11329-11330	NN	denotes	E
T11447	11331-11334	CC	denotes	and
T11448	11335-11336	NN	denotes	F
T11449	11336-11337	-RRB-	denotes	)
T11450	11337-11338	.	denotes	.
T11451	11338-11417	sentence	denotes	Original magnification: Panels A and C, 2X; panels B and D, 0.7X; E and F, 2X.
T11452	11339-11347	JJ	denotes	Original
T11453	11348-11361	NN	denotes	magnification
T11454	11361-11363	:	denotes	:
T11455	11363-11369	NNS	denotes	Panels
T11456	11370-11371	NN	denotes	A
T11458	11372-11375	CC	denotes	and
T11459	11376-11377	NN	denotes	C
T11460	11377-11379	,	denotes	,
T11457	11379-11381	NN	denotes	2X
T11462	11381-11382	:	denotes	;
T11463	11383-11389	NNS	denotes	panels
T11464	11390-11391	NN	denotes	B
T11466	11392-11395	CC	denotes	and
T11467	11396-11397	NN	denotes	D
T11468	11397-11399	,	denotes	,
T11465	11399-11403	NN	denotes	0.7X
T11469	11403-11404	:	denotes	;
T11470	11405-11406	NN	denotes	E
T11471	11407-11410	CC	denotes	and
T11472	11411-11412	NN	denotes	F
T11473	11412-11414	,	denotes	,
T11461	11414-11416	NN	denotes	2X
T11474	11416-11417	.	denotes	.
T11475	11417-11586	sentence	denotes	Labels: r, right; l, left; b, bronchial; br, bronchiole; e, esophagus; pa, pulmonary artery; ra, right atrium; la, left atrium; rv, right ventricle; lv, left ventricle.
T11476	11418-11424	NNS	denotes	Labels
T11478	11424-11426	:	denotes	:
T11477	11426-11427	NN	denotes	r
T11479	11427-11429	,	denotes	,
T11480	11429-11434	JJ	denotes	right
T11481	11434-11435	:	denotes	;
T11482	11436-11437	NN	denotes	l
T11483	11437-11439	,	denotes	,
T11484	11439-11443	JJ	denotes	left
T11485	11443-11444	:	denotes	;
T11486	11445-11446	NN	denotes	b
T11487	11446-11448	,	denotes	,
T11488	11448-11457	JJ	denotes	bronchial
T11489	11457-11458	:	denotes	;
T11490	11459-11461	NN	denotes	br
T11491	11461-11463	,	denotes	,
T11492	11463-11473	NN	denotes	bronchiole
T11493	11473-11474	:	denotes	;
T11494	11475-11476	NN	denotes	e
T11495	11476-11478	,	denotes	,
T11496	11478-11487	NN	denotes	esophagus
T11497	11487-11488	:	denotes	;
T11498	11489-11491	NN	denotes	pa
T11499	11491-11493	,	denotes	,
T11500	11493-11502	JJ	denotes	pulmonary
T11501	11503-11509	NN	denotes	artery
T11502	11509-11510	:	denotes	;
T11503	11511-11513	NN	denotes	ra
T11504	11513-11515	,	denotes	,
T11505	11515-11520	JJ	denotes	right
T11506	11521-11527	NN	denotes	atrium
T11507	11527-11528	:	denotes	;
T11508	11529-11531	NN	denotes	la
T11509	11531-11533	,	denotes	,
T11510	11533-11537	JJ	denotes	left
T11511	11538-11544	NN	denotes	atrium
T11512	11544-11545	:	denotes	;
T11513	11546-11548	NN	denotes	rv
T11514	11548-11550	,	denotes	,
T11515	11550-11555	JJ	denotes	right
T11516	11556-11565	NN	denotes	ventricle
T11517	11565-11566	:	denotes	;
T11518	11567-11569	NN	denotes	lv
T11519	11569-11571	,	denotes	,
T11520	11571-11575	JJ	denotes	left
T11521	11576-11585	NN	denotes	ventricle
T11522	11585-11586	.	denotes	.
T2762	11587-11589	JJ	denotes	We
T2761	11587-11591	sentence	denotes	We e
T2763	11590-11591	NNS	denotes	e
T2765	11591-11592	JJ	denotes	x
T2764	11591-11593	sentence	denotes	xa
T2766	11592-11593	NN	denotes	a
T2768	11593-11594	IN	denotes	m
T2767	11593-11597	sentence	denotes	mine
T2769	11594-11595	JJ	denotes	i
T2770	11595-11596	CC	denotes	n
T2771	11596-11597	NN	denotes	e
T2774	11597-11598	JJ	denotes	d
T2772	11597-11615	sentence	denotes	d the lungs for an
T2775	11599-11600	IN	denotes	t
T2776	11600-11601	NN	denotes	h
T2773	11601-11602	VBD	denotes	e
T2777	11603-11604	JJ	denotes	l
T2778	11604-11605	CC	denotes	u
T2779	11605-11608	NNS	denotes	ngs
T2781	11609-11611	IN	denotes	fo
T2780	11611-11612	VBD	denotes	r
T2782	11613-11614	JJR	denotes	a
T2783	11614-11615	IN	denotes	n
T2785	11615-11616	JJ	denotes	y
T2784	11615-11619	sentence	denotes	y gr
T2786	11617-11618	JJ	denotes	g
T2787	11618-11619	NN	denotes	r
T2789	11619-11620	IN	denotes	o
T2788	11619-11651	sentence	denotes	oss morphological abnormalities.
T2791	11620-11622	JJ	denotes	ss
T2792	11623-11636	JJ	denotes	morphological
T2790	11637-11650	NNS	denotes	abnormalities
T2793	11650-11651	.	denotes	.
T2794	11651-11843	sentence	denotes	The overall organogenesis of lungs was preserved in Dhcr7-/- pups; four right lung lobes and a single left lobe flanking the heart were easily seen on external examination at birth (Fig. 2D).
T2795	11652-11655	DT	denotes	The
T2797	11656-11663	JJ	denotes	overall
T2796	11664-11677	NN	denotes	organogenesis
T2799	11678-11680	IN	denotes	of
T2800	11681-11686	NNS	denotes	lungs
T2801	11687-11690	VBD	denotes	was
T2798	11691-11700	VBN	denotes	preserved
T2803	11701-11703	IN	denotes	in
T2804	11704-11709	NN	denotes	Dhcr7
T2806	11709-11710	SYM	denotes	-
T2807	11710-11711	SYM	denotes	/
T2808	11711-11712	SYM	denotes	-
T2805	11713-11717	NNS	denotes	pups
T2809	11717-11718	:	denotes	;
T2810	11719-11723	CD	denotes	four
T2812	11724-11729	JJ	denotes	right
T2813	11730-11734	NN	denotes	lung
T2811	11735-11740	NNS	denotes	lobes
T2814	11741-11744	CC	denotes	and
T2815	11745-11746	DT	denotes	a
T2817	11747-11753	JJ	denotes	single
T2818	11754-11758	JJ	denotes	left
T2816	11759-11763	NN	denotes	lobe
T2819	11764-11772	VBG	denotes	flanking
T2820	11773-11776	DT	denotes	the
T2821	11777-11782	NN	denotes	heart
T2822	11783-11787	VBD	denotes	were
T2823	11788-11794	RB	denotes	easily
T2802	11795-11799	VBN	denotes	seen
T2824	11800-11802	IN	denotes	on
T2825	11803-11811	JJ	denotes	external
T2826	11812-11823	NN	denotes	examination
T2827	11824-11826	IN	denotes	at
T2828	11827-11832	NN	denotes	birth
T2829	11833-11834	-LRB-	denotes	(
T2830	11834-11838	NN	denotes	Fig.
T2831	11839-11841	CD	denotes	2D
T2832	11841-11842	-RRB-	denotes	)
T2833	11842-11843	.	denotes	.
T2834	11843-11964	sentence	denotes	Examination for lobation at E13.5 also showed comparable gross anatomy (Fig. 2C) between wild-type and knockout embryos.
T2835	11844-11855	NN	denotes	Examination
T2837	11856-11859	IN	denotes	for
T2838	11860-11868	NN	denotes	lobation
T2839	11869-11871	IN	denotes	at
T2840	11872-11877	NN	denotes	E13.5
T2841	11878-11882	RB	denotes	also
T2836	11883-11889	VBD	denotes	showed
T2842	11890-11900	JJ	denotes	comparable
T2844	11901-11906	JJ	denotes	gross
T2843	11907-11914	NN	denotes	anatomy
T2845	11915-11916	-LRB-	denotes	(
T2846	11916-11920	NN	denotes	Fig.
T2847	11921-11923	CD	denotes	2C
T2848	11923-11924	-RRB-	denotes	)
T2849	11925-11932	IN	denotes	between
T2850	11933-11937	JJ	denotes	wild
T2852	11937-11938	HYPH	denotes	-
T2851	11938-11942	NN	denotes	type
T2854	11943-11946	CC	denotes	and
T2855	11947-11955	NN	denotes	knockout
T2853	11956-11963	NNS	denotes	embryos
T2856	11963-11964	.	denotes	.
T2857	11964-12169	sentence	denotes	Histological analyses of the cardiopulmonary structures at E20.5, taken just before birth to avoid artifacts from lack of lung inflation, also confirmed normal gross anatomic development (Fig. 2E and 2F).
T2858	11965-11977	JJ	denotes	Histological
T2859	11978-11986	NNS	denotes	analyses
T2861	11987-11989	IN	denotes	of
T2862	11990-11993	DT	denotes	the
T2864	11994-12009	JJ	denotes	cardiopulmonary
T2863	12010-12020	NNS	denotes	structures
T2865	12021-12023	IN	denotes	at
T2866	12024-12029	NN	denotes	E20.5
T2867	12029-12031	,	denotes	,
T2868	12031-12036	VBN	denotes	taken
T2869	12037-12041	RB	denotes	just
T2870	12042-12048	IN	denotes	before
T2871	12049-12054	NN	denotes	birth
T2872	12055-12057	TO	denotes	to
T2873	12058-12063	VB	denotes	avoid
T2874	12064-12073	NNS	denotes	artifacts
T2875	12074-12078	IN	denotes	from
T2876	12079-12083	NN	denotes	lack
T2877	12084-12086	IN	denotes	of
T2878	12087-12091	NN	denotes	lung
T2879	12092-12101	NN	denotes	inflation
T2880	12101-12103	,	denotes	,
T2881	12103-12107	RB	denotes	also
T2860	12108-12117	VBD	denotes	confirmed
T2882	12118-12124	JJ	denotes	normal
T2884	12125-12130	JJ	denotes	gross
T2885	12131-12139	JJ	denotes	anatomic
T2883	12140-12151	NN	denotes	development
T2886	12152-12153	-LRB-	denotes	(
T2888	12153-12157	NN	denotes	Fig.
T2887	12158-12160	CD	denotes	2E
T2889	12161-12164	CC	denotes	and
T2890	12165-12167	CD	denotes	2F
T2891	12167-12168	-RRB-	denotes	)
T2892	12168-12169	.	denotes	.
T2893	12169-12298	sentence	denotes	Additionally, no cardiac defects were identified and this was confirmed by more detailed histological analyses (data not shown).
T2894	12170-12182	RB	denotes	Additionally
T2896	12182-12184	,	denotes	,
T2897	12184-12186	DT	denotes	no
T2899	12187-12194	JJ	denotes	cardiac
T2898	12195-12202	NNS	denotes	defects
T2900	12203-12207	VBD	denotes	were
T2895	12208-12218	VBN	denotes	identified
T2901	12219-12222	CC	denotes	and
T2902	12223-12227	DT	denotes	this
T2904	12228-12231	VBD	denotes	was
T2903	12232-12241	VBN	denotes	confirmed
T2905	12242-12244	IN	denotes	by
T2906	12245-12249	RBR	denotes	more
T2907	12250-12258	JJ	denotes	detailed
T2909	12259-12271	JJ	denotes	histological
T2908	12272-12280	NNS	denotes	analyses
T2910	12281-12282	-LRB-	denotes	(
T2912	12282-12286	NNS	denotes	data
T2913	12287-12290	RB	denotes	not
T2911	12291-12296	VBN	denotes	shown
T2914	12296-12297	-RRB-	denotes	)
T2915	12297-12298	.	denotes	.
T2916	12298-12471	sentence	denotes	Thus, despite the obvious hypoxia frequently observed at birth of the knockout pups, major cardiac structural abnormalities were not observed to account for this phenotype.
T2917	12299-12303	RB	denotes	Thus
T2919	12303-12305	,	denotes	,
T2920	12305-12312	IN	denotes	despite
T2921	12313-12316	DT	denotes	the
T2923	12317-12324	JJ	denotes	obvious
T2922	12325-12332	NN	denotes	hypoxia
T2924	12333-12343	RB	denotes	frequently
T2925	12344-12352	VBN	denotes	observed
T2926	12353-12355	IN	denotes	at
T2927	12356-12361	NN	denotes	birth
T2928	12362-12364	IN	denotes	of
T2929	12365-12368	DT	denotes	the
T2931	12369-12377	NN	denotes	knockout
T2930	12378-12382	NNS	denotes	pups
T2932	12382-12384	,	denotes	,
T2933	12384-12389	JJ	denotes	major
T2935	12390-12397	JJ	denotes	cardiac
T2936	12398-12408	JJ	denotes	structural
T2934	12409-12422	NNS	denotes	abnormalities
T2937	12423-12427	VBD	denotes	were
T2938	12428-12431	RB	denotes	not
T2918	12432-12440	VBN	denotes	observed
T2939	12441-12443	TO	denotes	to
T2940	12444-12451	VB	denotes	account
T2941	12452-12455	IN	denotes	for
T2942	12456-12460	DT	denotes	this
T2943	12461-12470	NN	denotes	phenotype
T2944	12470-12471	.	denotes	.
T2945	12471-12705	sentence	denotes	No distinguishable morphological differences were discernable between Dhcr7-/- and wild-type lungs at E10.5, E13.5, E14.5 and E16.5 at low (10X) or high (40X) magnifications (Fig. 3, panel A: a-h, data for E10.5 and E13.5 not shown).
T2946	12472-12474	DT	denotes	No
T2948	12475-12490	JJ	denotes	distinguishable
T2949	12491-12504	JJ	denotes	morphological
T2947	12505-12516	NNS	denotes	differences
T2950	12517-12521	VBD	denotes	were
T2951	12522-12533	JJ	denotes	discernable
T2952	12534-12541	IN	denotes	between
T2953	12542-12547	NN	denotes	Dhcr7
T2955	12547-12548	SYM	denotes	-
T2956	12548-12549	SYM	denotes	/
T2957	12549-12550	SYM	denotes	-
T2958	12551-12554	CC	denotes	and
T2959	12555-12559	JJ	denotes	wild
T2961	12559-12560	HYPH	denotes	-
T2960	12560-12564	NN	denotes	type
T2954	12565-12570	NNS	denotes	lungs
T2962	12571-12573	IN	denotes	at
T2963	12574-12579	NN	denotes	E10.5
T2964	12579-12581	,	denotes	,
T2965	12581-12586	NN	denotes	E13.5
T2966	12586-12588	,	denotes	,
T2967	12588-12593	NN	denotes	E14.5
T2968	12594-12597	CC	denotes	and
T2969	12598-12603	NN	denotes	E16.5
T2970	12604-12606	IN	denotes	at
T2971	12607-12610	JJ	denotes	low
T2973	12611-12612	-LRB-	denotes	(
T2974	12612-12615	NN	denotes	10X
T2975	12615-12616	-RRB-	denotes	)
T2976	12617-12619	CC	denotes	or
T2977	12620-12624	JJ	denotes	high
T2978	12625-12626	-LRB-	denotes	(
T2979	12626-12629	NN	denotes	40X
T2980	12629-12630	-RRB-	denotes	)
T2972	12631-12645	NNS	denotes	magnifications
T2981	12646-12647	-LRB-	denotes	(
T2983	12647-12651	NN	denotes	Fig.
T2984	12652-12653	CD	denotes	3
T2985	12653-12655	,	denotes	,
T2986	12655-12660	NN	denotes	panel
T2982	12661-12662	NN	denotes	A
T2987	12662-12664	:	denotes	:
T2988	12664-12665	LS	denotes	a
T2989	12665-12666	HYPH	denotes	-
T2990	12666-12667	LS	denotes	h
T2991	12667-12669	,	denotes	,
T2992	12669-12673	NNS	denotes	data
T2994	12674-12677	IN	denotes	for
T2995	12678-12683	NN	denotes	E10.5
T2996	12684-12687	CC	denotes	and
T2997	12688-12693	NN	denotes	E13.5
T2998	12694-12697	RB	denotes	not
T2993	12698-12703	VBN	denotes	shown
T2999	12703-12704	-RRB-	denotes	)
T3000	12704-12705	.	denotes	.
T3001	12705-12882	sentence	denotes	However, from E17.5 onwards, lungs from knockout embryos appeared to have less saccular space and relatively more mesenchyme compared to wild-type lungs (Fig. 3, panel A: I-x).
T3002	12706-12713	RB	denotes	However
T3004	12713-12715	,	denotes	,
T3005	12715-12719	IN	denotes	from
T3006	12720-12725	NN	denotes	E17.5
T3007	12726-12733	RB	denotes	onwards
T3008	12733-12735	,	denotes	,
T3009	12735-12740	NNS	denotes	lungs
T3010	12741-12745	IN	denotes	from
T3011	12746-12754	NN	denotes	knockout
T3012	12755-12762	NNS	denotes	embryos
T3003	12763-12771	VBD	denotes	appeared
T3013	12772-12774	TO	denotes	to
T3014	12775-12779	VB	denotes	have
T3015	12780-12784	RBR	denotes	less
T3017	12785-12793	JJ	denotes	saccular
T3016	12794-12799	NN	denotes	space
T3018	12800-12803	CC	denotes	and
T3019	12804-12814	RB	denotes	relatively
T3020	12815-12819	JJR	denotes	more
T3021	12820-12830	NN	denotes	mesenchyme
T3022	12831-12839	VBN	denotes	compared
T3023	12840-12842	IN	denotes	to
T3024	12843-12847	JJ	denotes	wild
T3026	12847-12848	HYPH	denotes	-
T3025	12848-12852	NN	denotes	type
T3027	12853-12858	NNS	denotes	lungs
T3028	12859-12860	-LRB-	denotes	(
T3030	12860-12864	NN	denotes	Fig.
T3031	12865-12866	CD	denotes	3
T3032	12866-12868	,	denotes	,
T3033	12868-12873	NN	denotes	panel
T3029	12874-12875	NN	denotes	A
T3034	12875-12877	:	denotes	:
T3035	12877-12878	LS	denotes	I
T3036	12878-12879	SYM	denotes	-
T3037	12879-12880	LS	denotes	x
T3038	12880-12881	-RRB-	denotes	)
T3039	12881-12882	.	denotes	.
T3040	12882-13038	sentence	denotes	Lungs from control animals showed normal progression with formation of sac-like structures, the precursors of the alveoli (Fig. 3, panel A: i, m, q and u).
T3041	12883-12888	NNS	denotes	Lungs
T3043	12889-12893	IN	denotes	from
T3044	12894-12901	NN	denotes	control
T3045	12902-12909	NNS	denotes	animals
T3042	12910-12916	VBD	denotes	showed
T3046	12917-12923	JJ	denotes	normal
T3047	12924-12935	NN	denotes	progression
T3048	12936-12940	IN	denotes	with
T3049	12941-12950	NN	denotes	formation
T3050	12951-12953	IN	denotes	of
T3051	12954-12957	NN	denotes	sac
T3053	12957-12958	HYPH	denotes	-
T3052	12958-12962	JJ	denotes	like
T3054	12963-12973	NNS	denotes	structures
T3055	12973-12975	,	denotes	,
T3056	12975-12978	DT	denotes	the
T3057	12979-12989	NNS	denotes	precursors
T3058	12990-12992	IN	denotes	of
T3059	12993-12996	DT	denotes	the
T3060	12997-13004	NNS	denotes	alveoli
T3061	13005-13006	-LRB-	denotes	(
T3063	13006-13010	NN	denotes	Fig.
T3064	13011-13012	CD	denotes	3
T3065	13012-13014	,	denotes	,
T3066	13014-13019	NN	denotes	panel
T3062	13020-13021	NN	denotes	A
T3067	13021-13023	:	denotes	:
T3068	13023-13024	LS	denotes	i
T3069	13024-13026	,	denotes	,
T3070	13026-13027	LS	denotes	m
T3071	13027-13029	,	denotes	,
T3072	13029-13030	LS	denotes	q
T3073	13031-13034	CC	denotes	and
T3074	13035-13036	LS	denotes	u
T3075	13036-13037	-RRB-	denotes	)
T3076	13037-13038	.	denotes	.
T3077	13038-13195	sentence	denotes	In lungs from knockout embryos, expansion of epithelial tubules occurred but was significantly delayed (Fig. 3, cf. panel A: j, n, r, v with i, m, q and u).
T3078	13039-13041	IN	denotes	In
T3080	13042-13047	NNS	denotes	lungs
T3081	13048-13052	IN	denotes	from
T3082	13053-13061	NN	denotes	knockout
T3083	13062-13069	NNS	denotes	embryos
T3084	13069-13071	,	denotes	,
T3085	13071-13080	NN	denotes	expansion
T3086	13081-13083	IN	denotes	of
T3087	13084-13094	JJ	denotes	epithelial
T3088	13095-13102	NNS	denotes	tubules
T3079	13103-13111	VBD	denotes	occurred
T3089	13112-13115	CC	denotes	but
T3090	13116-13119	VBD	denotes	was
T3092	13120-13133	RB	denotes	significantly
T3091	13134-13141	VBN	denotes	delayed
T3093	13142-13143	-LRB-	denotes	(
T3095	13143-13147	NN	denotes	Fig.
T3096	13148-13149	CD	denotes	3
T3097	13149-13151	,	denotes	,
T3098	13151-13154	FW	denotes	cf.
T3100	13155-13160	NN	denotes	panel
T3099	13161-13162	NN	denotes	A
T3101	13162-13164	:	denotes	:
T3102	13164-13165	NN	denotes	j
T3103	13165-13167	,	denotes	,
T3104	13167-13168	NN	denotes	n
T3105	13168-13170	,	denotes	,
T3106	13170-13171	NN	denotes	r
T3107	13171-13173	,	denotes	,
T3094	13173-13174	NN	denotes	v
T3108	13175-13179	IN	denotes	with
T3109	13180-13181	NN	denotes	i
T3110	13181-13183	,	denotes	,
T3111	13183-13184	NN	denotes	m
T3112	13184-13186	,	denotes	,
T3113	13186-13187	NN	denotes	q
T3114	13188-13191	CC	denotes	and
T3115	13192-13193	NN	denotes	u
T3116	13193-13194	-RRB-	denotes	)
T3117	13194-13195	.	denotes	.
T3118	13195-13433	sentence	denotes	Morphometric measurements of lung sections at E17.5, E18.5, E20.5 and in lungs from neonates showed that the proportion of terminal sac space area was significantly decreased in Dhcr7-/- lungs, compared wild-type lungs (Fig. 3, panel B).
T3119	13196-13208	JJ	denotes	Morphometric
T3120	13209-13221	NNS	denotes	measurements
T3122	13222-13224	IN	denotes	of
T3123	13225-13229	NN	denotes	lung
T3124	13230-13238	NNS	denotes	sections
T3125	13239-13241	IN	denotes	at
T3126	13242-13247	NN	denotes	E17.5
T3128	13247-13249	,	denotes	,
T3129	13249-13254	NN	denotes	E18.5
T3130	13254-13256	,	denotes	,
T3127	13256-13261	NN	denotes	E20.5
T3131	13262-13265	CC	denotes	and
T3132	13266-13268	IN	denotes	in
T3133	13269-13274	NNS	denotes	lungs
T3134	13275-13279	IN	denotes	from
T3135	13280-13288	NNS	denotes	neonates
T3121	13289-13295	VBD	denotes	showed
T3136	13296-13300	IN	denotes	that
T3138	13301-13304	DT	denotes	the
T3139	13305-13315	NN	denotes	proportion
T3140	13316-13318	IN	denotes	of
T3141	13319-13327	JJ	denotes	terminal
T3143	13328-13331	NN	denotes	sac
T3142	13332-13337	NN	denotes	space
T3144	13338-13342	NN	denotes	area
T3145	13343-13346	VBD	denotes	was
T3146	13347-13360	RB	denotes	significantly
T3137	13361-13370	VBN	denotes	decreased
T3147	13371-13373	IN	denotes	in
T3148	13374-13379	NN	denotes	Dhcr7
T3150	13379-13380	SYM	denotes	-
T3151	13380-13381	SYM	denotes	/
T3152	13381-13382	SYM	denotes	-
T3149	13383-13388	NNS	denotes	lungs
T3153	13388-13390	,	denotes	,
T3154	13390-13398	VBN	denotes	compared
T3155	13399-13403	JJ	denotes	wild
T3157	13403-13404	HYPH	denotes	-
T3156	13404-13408	NN	denotes	type
T3158	13409-13414	NNS	denotes	lungs
T3159	13415-13416	-LRB-	denotes	(
T3161	13416-13420	NN	denotes	Fig.
T3162	13421-13422	CD	denotes	3
T3163	13422-13424	,	denotes	,
T3164	13424-13429	NN	denotes	panel
T3160	13430-13431	NN	denotes	B
T3165	13431-13432	-RRB-	denotes	)
T3166	13432-13433	.	denotes	.
T3167	13433-13534	sentence	denotes	Interstitial mesenchyme appeared thicker and the lungs had less septation compared to control lungs.
T3168	13434-13446	JJ	denotes	Interstitial
T3169	13447-13457	NN	denotes	mesenchyme
T3170	13458-13466	VBD	denotes	appeared
T3171	13467-13474	JJR	denotes	thicker
T3172	13475-13478	CC	denotes	and
T3173	13479-13482	DT	denotes	the
T3174	13483-13488	NNS	denotes	lungs
T3175	13489-13492	VBD	denotes	had
T3176	13493-13497	JJR	denotes	less
T3177	13498-13507	NN	denotes	septation
T3178	13508-13516	VBN	denotes	compared
T3179	13517-13519	IN	denotes	to
T3180	13520-13527	NN	denotes	control
T3181	13528-13533	NNS	denotes	lungs
T3182	13533-13534	.	denotes	.
T3183	13534-13634	sentence	denotes	This 'thickening' of the alveoli may explain the hypoxia frequently seen in Dhcr7-/- pups at birth.
T3184	13535-13539	DT	denotes	This
T3186	13540-13541	``	denotes	'
T3185	13541-13551	NN	denotes	thickening
T3188	13551-13552	''	denotes	'
T3189	13553-13555	IN	denotes	of
T3190	13556-13559	DT	denotes	the
T3191	13560-13567	NNS	denotes	alveoli
T3192	13568-13571	MD	denotes	may
T3187	13572-13579	VB	denotes	explain
T3193	13580-13583	DT	denotes	the
T3194	13584-13591	NN	denotes	hypoxia
T3195	13592-13602	RB	denotes	frequently
T3196	13603-13607	VBN	denotes	seen
T3197	13608-13610	IN	denotes	in
T3198	13611-13616	NN	denotes	Dhcr7
T3200	13616-13617	SYM	denotes	-
T3201	13617-13618	SYM	denotes	/
T3202	13618-13619	SYM	denotes	-
T3199	13620-13624	NNS	denotes	pups
T3203	13625-13627	IN	denotes	at
T3204	13628-13633	NN	denotes	birth
T3205	13633-13634	.	denotes	.
T11605	13645-13657	JJ	denotes	Histological
T11606	13658-13666	NNS	denotes	analyses
T11607	13667-13669	IN	denotes	of
T11608	13670-13675	NNS	denotes	lungs
T11609	13676-13678	IN	denotes	at
T11610	13679-13686	JJ	denotes	various
T11612	13687-13696	JJ	denotes	embryonic
T11611	13697-13703	NNS	denotes	stages
T11613	13703-13704	.	denotes	.
T11614	13704-13812	sentence	denotes	Panel A indicates lung sections stained with H and E, and taken at various gestational stages as indicated.
T11615	13705-13710	NN	denotes	Panel
T11616	13711-13712	NN	denotes	A
T11617	13713-13722	VBZ	denotes	indicates
T11618	13723-13727	NN	denotes	lung
T11619	13728-13736	NNS	denotes	sections
T11620	13737-13744	VBN	denotes	stained
T11621	13745-13749	IN	denotes	with
T11622	13750-13751	NN	denotes	H
T11623	13752-13755	CC	denotes	and
T11624	13756-13757	NN	denotes	E
T11625	13757-13759	,	denotes	,
T11626	13759-13762	CC	denotes	and
T11627	13763-13768	VBN	denotes	taken
T11628	13769-13771	IN	denotes	at
T11629	13772-13779	JJ	denotes	various
T11631	13780-13791	JJ	denotes	gestational
T11630	13792-13798	NNS	denotes	stages
T11632	13799-13801	IN	denotes	as
T11633	13802-13811	VBN	denotes	indicated
T11634	13811-13812	.	denotes	.
T11635	13812-13923	sentence	denotes	The first two columns are at lower magnification (10X) and the last two columns at higher magnification (40X).
T11636	13813-13816	DT	denotes	The
T11638	13817-13822	JJ	denotes	first
T11639	13823-13826	CD	denotes	two
T11637	13827-13834	NNS	denotes	columns
T11640	13835-13838	VBP	denotes	are
T11641	13839-13841	IN	denotes	at
T11642	13842-13847	JJR	denotes	lower
T11643	13848-13861	NN	denotes	magnification
T11644	13862-13863	-LRB-	denotes	(
T11645	13863-13866	NN	denotes	10X
T11646	13866-13867	-RRB-	denotes	)
T11647	13868-13871	CC	denotes	and
T11648	13872-13875	DT	denotes	the
T11650	13876-13880	JJ	denotes	last
T11651	13881-13884	CD	denotes	two
T11649	13885-13892	NNS	denotes	columns
T11652	13893-13895	IN	denotes	at
T11653	13896-13902	JJR	denotes	higher
T11654	13903-13916	NN	denotes	magnification
T11655	13917-13918	-LRB-	denotes	(
T11656	13918-13921	NN	denotes	40X
T11657	13921-13922	-RRB-	denotes	)
T11658	13922-13923	.	denotes	.
T11659	13923-14071	sentence	denotes	The first and third columns are representative sections from wild-type (+/+) embryos and the second and fourth columns from knockout (-/-) embryos.
T11660	13924-13927	DT	denotes	The
T11662	13928-13933	JJ	denotes	first
T11663	13934-13937	CC	denotes	and
T11664	13938-13943	JJ	denotes	third
T11661	13944-13951	NNS	denotes	columns
T11665	13952-13955	VBP	denotes	are
T11666	13956-13970	JJ	denotes	representative
T11667	13971-13979	NNS	denotes	sections
T11668	13980-13984	IN	denotes	from
T11669	13985-13989	JJ	denotes	wild
T11671	13989-13990	HYPH	denotes	-
T11670	13990-13994	NN	denotes	type
T11673	13995-13996	-LRB-	denotes	(
T11674	13996-13997	SYM	denotes	+
T11676	13997-13998	SYM	denotes	/
T11675	13998-13999	SYM	denotes	+
T11677	13999-14000	-RRB-	denotes	)
T11672	14001-14008	NNS	denotes	embryos
T11678	14009-14012	CC	denotes	and
T11679	14013-14016	DT	denotes	the
T11681	14017-14023	JJ	denotes	second
T11682	14024-14027	CC	denotes	and
T11683	14028-14034	JJ	denotes	fourth
T11680	14035-14042	NNS	denotes	columns
T11684	14043-14047	IN	denotes	from
T11685	14048-14056	NN	denotes	knockout
T11687	14057-14058	-LRB-	denotes	(
T11688	14058-14059	SYM	denotes	-
T11690	14059-14060	SYM	denotes	/
T11689	14060-14061	SYM	denotes	-
T11691	14061-14062	-RRB-	denotes	)
T11686	14063-14070	NNS	denotes	embryos
T11692	14070-14071	.	denotes	.
T11693	14071-14133	sentence	denotes	No differences were visible at E14.5 or E16.5 (panel A, a-h).
T11694	14072-14074	DT	denotes	No
T11695	14075-14086	NNS	denotes	differences
T11696	14087-14091	VBD	denotes	were
T11697	14092-14099	JJ	denotes	visible
T11698	14100-14102	IN	denotes	at
T11699	14103-14108	NN	denotes	E14.5
T11700	14109-14111	CC	denotes	or
T11701	14112-14117	NN	denotes	E16.5
T11702	14118-14119	-LRB-	denotes	(
T11704	14119-14124	NN	denotes	panel
T11703	14125-14126	NN	denotes	A
T11705	14126-14128	,	denotes	,
T11706	14128-14129	LS	denotes	a
T11707	14129-14130	SYM	denotes	-
T11708	14130-14131	LS	denotes	h
T11709	14131-14132	-RRB-	denotes	)
T11710	14132-14133	.	denotes	.
T11711	14133-14205	sentence	denotes	Significant differences were visible from E17.5 onwards (panel A, i-x).
T11712	14134-14145	JJ	denotes	Significant
T11713	14146-14157	NNS	denotes	differences
T11714	14158-14162	VBD	denotes	were
T11715	14163-14170	JJ	denotes	visible
T11716	14171-14175	IN	denotes	from
T11717	14176-14181	NN	denotes	E17.5
T11718	14182-14189	RB	denotes	onwards
T11719	14190-14191	-LRB-	denotes	(
T11721	14191-14196	NN	denotes	panel
T11720	14197-14198	NN	denotes	A
T11722	14198-14200	,	denotes	,
T11723	14200-14201	LS	denotes	i
T11724	14201-14202	SYM	denotes	-
T11725	14202-14203	LS	denotes	x
T11726	14203-14204	-RRB-	denotes	)
T11727	14204-14205	.	denotes	.
T11728	14205-14435	sentence	denotes	Distal tubules showed dilation and mesenchyme thinning, with progression of septation in wild-type lungs (panel A, i, m and q), whereas the knockout lungs showed less saccular structures and more mesenchyme (panel A, j, n and r).
T11729	14206-14212	JJ	denotes	Distal
T11730	14213-14220	NNS	denotes	tubules
T11731	14221-14227	VBD	denotes	showed
T11732	14228-14236	NN	denotes	dilation
T11733	14237-14240	CC	denotes	and
T11734	14241-14251	NN	denotes	mesenchyme
T11735	14252-14260	NN	denotes	thinning
T11736	14260-14262	,	denotes	,
T11737	14262-14266	IN	denotes	with
T11738	14267-14278	NN	denotes	progression
T11739	14279-14281	IN	denotes	of
T11740	14282-14291	NN	denotes	septation
T11741	14292-14294	IN	denotes	in
T11742	14295-14299	JJ	denotes	wild
T11744	14299-14300	HYPH	denotes	-
T11743	14300-14304	NN	denotes	type
T11745	14305-14310	NNS	denotes	lungs
T11746	14311-14312	-LRB-	denotes	(
T11748	14312-14317	NN	denotes	panel
T11747	14318-14319	NN	denotes	A
T11749	14319-14321	,	denotes	,
T11750	14321-14322	LS	denotes	i
T11751	14322-14324	,	denotes	,
T11752	14324-14325	LS	denotes	m
T11753	14326-14329	CC	denotes	and
T11754	14330-14331	LS	denotes	q
T11755	14331-14332	-RRB-	denotes	)
T11756	14332-14334	,	denotes	,
T11757	14334-14341	IN	denotes	whereas
T11759	14342-14345	DT	denotes	the
T11761	14346-14354	NN	denotes	knockout
T11760	14355-14360	NNS	denotes	lungs
T11758	14361-14367	VBD	denotes	showed
T11762	14368-14372	RBR	denotes	less
T11763	14373-14381	JJ	denotes	saccular
T11764	14382-14392	NNS	denotes	structures
T11765	14393-14396	CC	denotes	and
T11766	14397-14401	JJR	denotes	more
T11767	14402-14412	NN	denotes	mesenchyme
T11768	14413-14414	-LRB-	denotes	(
T11770	14414-14419	NN	denotes	panel
T11769	14420-14421	NN	denotes	A
T11771	14421-14423	,	denotes	,
T11772	14423-14424	LS	denotes	j
T11773	14424-14426	,	denotes	,
T11774	14426-14427	LS	denotes	n
T11775	14428-14431	CC	denotes	and
T11776	14432-14433	LS	denotes	r
T11777	14433-14434	-RRB-	denotes	)
T11778	14434-14435	.	denotes	.
T11779	14435-14677	sentence	denotes	At higher magnifications of wild-type lungs (panel A, k, o and s), developing pre-alveoli and thinning mesenchyme (arrowheads) were seen but sections from knockout embryos showed a delay in this sequence of development (panel A, l, p and t).
T11780	14436-14438	IN	denotes	At
T11782	14439-14445	JJR	denotes	higher
T11783	14446-14460	NNS	denotes	magnifications
T11784	14461-14463	IN	denotes	of
T11785	14464-14468	JJ	denotes	wild
T11787	14468-14469	HYPH	denotes	-
T11786	14469-14473	NN	denotes	type
T11788	14474-14479	NNS	denotes	lungs
T11789	14480-14481	-LRB-	denotes	(
T11791	14481-14486	NN	denotes	panel
T11790	14487-14488	NN	denotes	A
T11792	14488-14490	,	denotes	,
T11793	14490-14491	LS	denotes	k
T11794	14491-14493	,	denotes	,
T11795	14493-14494	LS	denotes	o
T11796	14495-14498	CC	denotes	and
T11797	14499-14500	LS	denotes	s
T11798	14500-14501	-RRB-	denotes	)
T11799	14501-14503	,	denotes	,
T11800	14503-14513	VBG	denotes	developing
T11801	14514-14525	NN	denotes	pre-alveoli
T11802	14526-14529	CC	denotes	and
T11803	14530-14538	VBG	denotes	thinning
T11804	14539-14549	NN	denotes	mesenchyme
T11805	14550-14551	-LRB-	denotes	(
T11806	14551-14561	NNS	denotes	arrowheads
T11807	14561-14562	-RRB-	denotes	)
T11808	14563-14567	VBD	denotes	were
T11781	14568-14572	VBN	denotes	seen
T11809	14573-14576	CC	denotes	but
T11810	14577-14585	NNS	denotes	sections
T11812	14586-14590	IN	denotes	from
T11813	14591-14599	NN	denotes	knockout
T11814	14600-14607	NNS	denotes	embryos
T11811	14608-14614	VBD	denotes	showed
T11815	14615-14616	DT	denotes	a
T11816	14617-14622	NN	denotes	delay
T11817	14623-14625	IN	denotes	in
T11818	14626-14630	DT	denotes	this
T11819	14631-14639	NN	denotes	sequence
T11820	14640-14642	IN	denotes	of
T11821	14643-14654	NN	denotes	development
T11822	14655-14656	-LRB-	denotes	(
T11824	14656-14661	NN	denotes	panel
T11823	14662-14663	NN	denotes	A
T11825	14663-14665	,	denotes	,
T11826	14665-14666	LS	denotes	l
T11827	14666-14668	,	denotes	,
T11828	14668-14669	LS	denotes	p
T11829	14670-14673	CC	denotes	and
T11830	14674-14675	LS	denotes	t
T11831	14675-14676	-RRB-	denotes	)
T11832	14676-14677	.	denotes	.
T11833	14677-14793	sentence	denotes	At birth, lungs from knockout pups showed deficient septation and thick-walled mesenchyme (panel A, x, arrowheads).
T11834	14678-14680	IN	denotes	At
T11836	14681-14686	NN	denotes	birth
T11837	14686-14688	,	denotes	,
T11838	14688-14693	NNS	denotes	lungs
T11839	14694-14698	IN	denotes	from
T11840	14699-14707	NN	denotes	knockout
T11841	14708-14712	NNS	denotes	pups
T11835	14713-14719	VBD	denotes	showed
T11842	14720-14729	JJ	denotes	deficient
T11843	14730-14739	NN	denotes	septation
T11844	14740-14743	CC	denotes	and
T11845	14744-14749	JJ	denotes	thick
T11847	14749-14750	HYPH	denotes	-
T11846	14750-14756	VBN	denotes	walled
T11848	14757-14767	NN	denotes	mesenchyme
T11849	14768-14769	-LRB-	denotes	(
T11851	14769-14774	NN	denotes	panel
T11852	14775-14776	NN	denotes	A
T11853	14776-14778	,	denotes	,
T11854	14778-14779	LS	denotes	x
T11855	14779-14781	,	denotes	,
T11850	14781-14791	NNS	denotes	arrowheads
T11856	14791-14792	-RRB-	denotes	)
T11857	14792-14793	.	denotes	.
T11858	14793-14889	sentence	denotes	Labels: de, distal epithelium; m, mesenchyme; PA, pulmonary artery; a, pre-alveoli; b, bronchi.
T11859	14794-14800	NNS	denotes	Labels
T11861	14800-14802	:	denotes	:
T11860	14802-14804	NN	denotes	de
T11862	14804-14806	,	denotes	,
T11863	14806-14812	JJ	denotes	distal
T11864	14813-14823	NN	denotes	epithelium
T11865	14823-14824	:	denotes	;
T11866	14825-14826	NN	denotes	m
T11868	14826-14828	,	denotes	,
T11867	14828-14838	NN	denotes	mesenchyme
T11869	14838-14839	:	denotes	;
T11870	14840-14842	NN	denotes	PA
T11871	14842-14844	,	denotes	,
T11872	14844-14853	JJ	denotes	pulmonary
T11873	14854-14860	NN	denotes	artery
T11874	14860-14861	:	denotes	;
T11875	14862-14863	NN	denotes	a
T11876	14863-14865	,	denotes	,
T11877	14865-14876	NNS	denotes	pre-alveoli
T11878	14876-14877	:	denotes	;
T11879	14878-14879	NN	denotes	b
T11880	14879-14881	,	denotes	,
T11881	14881-14888	NNS	denotes	bronchi
T11882	14888-14889	.	denotes	.
T11883	14889-14997	sentence	denotes	Panel B shows the morphometric analyses of lung terminal sac spaces in lungs at various gestational stages.
T11884	14890-14895	NN	denotes	Panel
T11885	14896-14897	NN	denotes	B
T11886	14898-14903	VBZ	denotes	shows
T11887	14904-14907	DT	denotes	the
T11889	14908-14920	JJ	denotes	morphometric
T11888	14921-14929	NNS	denotes	analyses
T11890	14930-14932	IN	denotes	of
T11891	14933-14937	NN	denotes	lung
T11893	14938-14946	JJ	denotes	terminal
T11892	14947-14950	NN	denotes	sac
T11894	14951-14957	NNS	denotes	spaces
T11895	14958-14960	IN	denotes	in
T11896	14961-14966	NNS	denotes	lungs
T11897	14967-14969	IN	denotes	at
T11898	14970-14977	JJ	denotes	various
T11900	14978-14989	JJ	denotes	gestational
T11899	14990-14996	NNS	denotes	stages
T11901	14996-14997	.	denotes	.
T11902	14997-15077	sentence	denotes	A significant reduction in the terminal sac space was noted from E17.5 onwards.
T11903	14998-14999	DT	denotes	A
T11905	15000-15011	JJ	denotes	significant
T11904	15012-15021	NN	denotes	reduction
T11907	15022-15024	IN	denotes	in
T11908	15025-15028	DT	denotes	the
T11910	15029-15037	JJ	denotes	terminal
T11911	15038-15041	NN	denotes	sac
T11909	15042-15047	NN	denotes	space
T11912	15048-15051	VBD	denotes	was
T11906	15052-15057	VBN	denotes	noted
T11913	15058-15062	IN	denotes	from
T11914	15063-15068	NN	denotes	E17.5
T11915	15069-15076	RB	denotes	onwards
T11916	15076-15077	.	denotes	.
T11917	15077-15114	sentence	denotes	Results are expressed as mean ± s.d.
T11918	15078-15085	NNS	denotes	Results
T11920	15086-15089	VBP	denotes	are
T11919	15090-15099	VBN	denotes	expressed
T11921	15100-15102	IN	denotes	as
T11922	15103-15107	NN	denotes	mean
T11924	15108-15109	SYM	denotes	±
T11923	15110-15113	NN	denotes	s.d
T11925	15113-15114	.	denotes	.
T3338	15116-15126	VBN	denotes	Attenuated
T3340	15127-15131	NN	denotes	cell
T3339	15132-15145	NN	denotes	proliferation
T3341	15146-15148	IN	denotes	in
T3342	15149-15153	JJ	denotes	late
T3343	15154-15165	JJ	denotes	gestational
T3345	15166-15171	NN	denotes	Dhcr7
T3346	15171-15172	SYM	denotes	-
T3347	15172-15173	SYM	denotes	/
T3348	15173-15174	SYM	denotes	-
T3344	15175-15180	NNS	denotes	lungs
T3349	15180-15297	sentence	denotes	Does the loss of normal cholesterol biosynthesis lead to abnormalities of cell proliferation/division or cell death?
T3350	15181-15185	VBZ	denotes	Does
T3352	15186-15189	DT	denotes	the
T3353	15190-15194	NN	denotes	loss
T3354	15195-15197	IN	denotes	of
T3355	15198-15204	JJ	denotes	normal
T3357	15205-15216	NN	denotes	cholesterol
T3356	15217-15229	NN	denotes	biosynthesis
T3351	15230-15234	VB	denotes	lead
T3358	15235-15237	IN	denotes	to
T3359	15238-15251	NNS	denotes	abnormalities
T3360	15252-15254	IN	denotes	of
T3361	15255-15259	NN	denotes	cell
T3363	15260-15273	NN	denotes	proliferation
T3364	15273-15274	SYM	denotes	/
T3362	15274-15282	NN	denotes	division
T3365	15283-15285	CC	denotes	or
T3366	15286-15290	NN	denotes	cell
T3367	15291-15296	NN	denotes	death
T3368	15296-15297	.	denotes	?
T3369	15297-15388	sentence	denotes	Cell proliferation was examined by immunostaining for PCNA and also by BrdU incorporation.
T3370	15298-15302	NN	denotes	Cell
T3371	15303-15316	NN	denotes	proliferation
T3373	15317-15320	VBD	denotes	was
T3372	15321-15329	VBN	denotes	examined
T3374	15330-15332	IN	denotes	by
T3375	15333-15347	NN	denotes	immunostaining
T3376	15348-15351	IN	denotes	for
T3377	15352-15356	NN	denotes	PCNA
T3378	15357-15360	CC	denotes	and
T3379	15361-15365	RB	denotes	also
T3380	15366-15368	IN	denotes	by
T3381	15369-15373	NN	denotes	BrdU
T3382	15374-15387	NN	denotes	incorporation
T3383	15387-15388	.	denotes	.
T3384	15388-15543	sentence	denotes	Both PCNA and BrdU staining of wild-type and knockout lungs at early gestational stages (E13.5 and E14.5) showed comparable patterns (Fig. 4, panels A-D).
T3385	15389-15393	CC	denotes	Both
T3386	15394-15398	NN	denotes	PCNA
T3388	15399-15402	CC	denotes	and
T3389	15403-15407	NN	denotes	BrdU
T3387	15408-15416	NN	denotes	staining
T3391	15417-15419	IN	denotes	of
T3392	15420-15424	JJ	denotes	wild
T3394	15424-15425	HYPH	denotes	-
T3393	15425-15429	NN	denotes	type
T3396	15430-15433	CC	denotes	and
T3397	15434-15442	NN	denotes	knockout
T3395	15443-15448	NNS	denotes	lungs
T3398	15449-15451	IN	denotes	at
T3399	15452-15457	JJ	denotes	early
T3401	15458-15469	JJ	denotes	gestational
T3400	15470-15476	NNS	denotes	stages
T3402	15477-15478	-LRB-	denotes	(
T3403	15478-15483	NN	denotes	E13.5
T3404	15484-15487	CC	denotes	and
T3405	15488-15493	NN	denotes	E14.5
T3406	15493-15494	-RRB-	denotes	)
T3390	15495-15501	VBD	denotes	showed
T3407	15502-15512	JJ	denotes	comparable
T3408	15513-15521	NNS	denotes	patterns
T3409	15522-15523	-LRB-	denotes	(
T3411	15523-15527	NN	denotes	Fig.
T3412	15528-15529	CD	denotes	4
T3413	15529-15531	,	denotes	,
T3414	15531-15537	NNS	denotes	panels
T3410	15538-15539	NN	denotes	A
T3415	15539-15540	SYM	denotes	-
T3416	15540-15541	NN	denotes	D
T3417	15541-15542	-RRB-	denotes	)
T3418	15542-15543	.	denotes	.
T3419	15543-15713	sentence	denotes	However, a clear reduction of PCNA and BrdU labeling was visualized in distal lungs of knockout embryos at late gestational stages (E18.5 and E20.5, Fig. 4, panels E-H).
T3420	15544-15551	RB	denotes	However
T3422	15551-15553	,	denotes	,
T3423	15553-15554	DT	denotes	a
T3425	15555-15560	JJ	denotes	clear
T3424	15561-15570	NN	denotes	reduction
T3426	15571-15573	IN	denotes	of
T3427	15574-15578	NN	denotes	PCNA
T3429	15579-15582	CC	denotes	and
T3430	15583-15587	NN	denotes	BrdU
T3428	15588-15596	NN	denotes	labeling
T3431	15597-15600	VBD	denotes	was
T3421	15601-15611	VBN	denotes	visualized
T3432	15612-15614	IN	denotes	in
T3433	15615-15621	JJ	denotes	distal
T3434	15622-15627	NNS	denotes	lungs
T3435	15628-15630	IN	denotes	of
T3436	15631-15639	NN	denotes	knockout
T3437	15640-15647	NNS	denotes	embryos
T3438	15648-15650	IN	denotes	at
T3439	15651-15655	JJ	denotes	late
T3441	15656-15667	JJ	denotes	gestational
T3440	15668-15674	NNS	denotes	stages
T3442	15675-15676	-LRB-	denotes	(
T3444	15676-15681	NN	denotes	E18.5
T3445	15682-15685	CC	denotes	and
T3446	15686-15691	NN	denotes	E20.5
T3447	15691-15693	,	denotes	,
T3448	15693-15697	NN	denotes	Fig.
T3449	15698-15699	CD	denotes	4
T3450	15699-15701	,	denotes	,
T3451	15701-15707	NNS	denotes	panels
T3443	15708-15709	NN	denotes	E
T3452	15709-15710	SYM	denotes	-
T3453	15710-15711	NN	denotes	H
T3454	15711-15712	-RRB-	denotes	)
T3455	15712-15713	.	denotes	.
T3456	15713-15810	sentence	denotes	Attenuated PCNA labeling was also noted in knockout hearts at term (Fig. 4, cf. panels I and J).
T3457	15714-15724	VBN	denotes	Attenuated
T3459	15725-15729	NN	denotes	PCNA
T3458	15730-15738	NN	denotes	labeling
T3461	15739-15742	VBD	denotes	was
T3462	15743-15747	RB	denotes	also
T3460	15748-15753	VBN	denotes	noted
T3463	15754-15756	IN	denotes	in
T3464	15757-15765	NN	denotes	knockout
T3465	15766-15772	NNS	denotes	hearts
T3466	15773-15775	IN	denotes	at
T3467	15776-15780	NN	denotes	term
T3468	15781-15782	-LRB-	denotes	(
T3470	15782-15786	NN	denotes	Fig.
T3471	15787-15788	CD	denotes	4
T3472	15788-15790	,	denotes	,
T3473	15790-15793	FW	denotes	cf.
T3474	15794-15800	NNS	denotes	panels
T3469	15801-15802	NN	denotes	I
T3475	15803-15806	CC	denotes	and
T3476	15807-15808	NN	denotes	J
T3477	15808-15809	-RRB-	denotes	)
T3478	15809-15810	.	denotes	.
T3479	15810-16021	sentence	denotes	When lungs were stained for pHH3, a marker of active cell division, a clear reduction of pHH3 expression in the epithelium of knockout lung was observed at E20.5 but not at E14.5 (Fig. 4, panels K, L, O and P).
T3480	15811-15815	WRB	denotes	When
T3482	15816-15821	NNS	denotes	lungs
T3483	15822-15826	VBD	denotes	were
T3481	15827-15834	VBN	denotes	stained
T3485	15835-15838	IN	denotes	for
T3486	15839-15843	NN	denotes	pHH3
T3487	15843-15845	,	denotes	,
T3488	15845-15846	DT	denotes	a
T3489	15847-15853	NN	denotes	marker
T3490	15854-15856	IN	denotes	of
T3491	15857-15863	JJ	denotes	active
T3493	15864-15868	NN	denotes	cell
T3492	15869-15877	NN	denotes	division
T3494	15877-15879	,	denotes	,
T3495	15879-15880	DT	denotes	a
T3497	15881-15886	JJ	denotes	clear
T3496	15887-15896	NN	denotes	reduction
T3498	15897-15899	IN	denotes	of
T3499	15900-15904	NN	denotes	pHH3
T3500	15905-15915	NN	denotes	expression
T3501	15916-15918	IN	denotes	in
T3502	15919-15922	DT	denotes	the
T3503	15923-15933	NN	denotes	epithelium
T3504	15934-15936	IN	denotes	of
T3505	15937-15945	NN	denotes	knockout
T3506	15946-15950	NN	denotes	lung
T3507	15951-15954	VBD	denotes	was
T3484	15955-15963	VBN	denotes	observed
T3508	15964-15966	IN	denotes	at
T3509	15967-15972	NN	denotes	E20.5
T3510	15973-15976	CC	denotes	but
T3511	15977-15980	RB	denotes	not
T3512	15981-15983	IN	denotes	at
T3513	15984-15989	NN	denotes	E14.5
T3514	15990-15991	-LRB-	denotes	(
T3516	15991-15995	NN	denotes	Fig.
T3517	15996-15997	CD	denotes	4
T3518	15997-15999	,	denotes	,
T3519	15999-16005	NNS	denotes	panels
T3515	16006-16007	NN	denotes	K
T3520	16007-16009	,	denotes	,
T3521	16009-16010	NN	denotes	L
T3522	16010-16012	,	denotes	,
T3523	16012-16013	NN	denotes	O
T3524	16014-16017	CC	denotes	and
T3525	16018-16019	NN	denotes	P
T3526	16019-16020	-RRB-	denotes	)
T3527	16020-16021	.	denotes	.
T3528	16021-16195	sentence	denotes	In contrast, using the TUNEL assay, no apparent differences in cell apoptosis rates were observed between wild-type and knockout lungs at E18.5 (Fig. 4, cf. panels M and N).
T3529	16022-16024	IN	denotes	In
T3531	16025-16033	NN	denotes	contrast
T3532	16033-16035	,	denotes	,
T3533	16035-16040	VBG	denotes	using
T3534	16041-16044	DT	denotes	the
T3536	16045-16050	NN	denotes	TUNEL
T3535	16051-16056	NN	denotes	assay
T3537	16056-16058	,	denotes	,
T3538	16058-16060	DT	denotes	no
T3540	16061-16069	JJ	denotes	apparent
T3539	16070-16081	NNS	denotes	differences
T3541	16082-16084	IN	denotes	in
T3542	16085-16089	NN	denotes	cell
T3543	16090-16099	NN	denotes	apoptosis
T3544	16100-16105	NNS	denotes	rates
T3545	16106-16110	VBD	denotes	were
T3530	16111-16119	VBN	denotes	observed
T3546	16120-16127	IN	denotes	between
T3547	16128-16132	JJ	denotes	wild
T3549	16132-16133	HYPH	denotes	-
T3548	16133-16137	NN	denotes	type
T3551	16138-16141	CC	denotes	and
T3552	16142-16150	NN	denotes	knockout
T3550	16151-16156	NNS	denotes	lungs
T3553	16157-16159	IN	denotes	at
T3554	16160-16165	NN	denotes	E18.5
T3555	16166-16167	-LRB-	denotes	(
T3557	16167-16171	NN	denotes	Fig.
T3558	16172-16173	CD	denotes	4
T3559	16173-16175	,	denotes	,
T3560	16175-16178	FW	denotes	cf.
T3561	16179-16185	NNS	denotes	panels
T3556	16186-16187	NN	denotes	M
T3562	16188-16191	CC	denotes	and
T3563	16192-16193	NN	denotes	N
T3564	16193-16194	-RRB-	denotes	)
T3565	16194-16195	.	denotes	.
T3566	16195-16332	sentence	denotes	Thus, at the late gestational stages, cell proliferation/division was impaired in knockout embryos with no increase in cell death rates.
T3567	16196-16200	RB	denotes	Thus
T3569	16200-16202	,	denotes	,
T3570	16202-16204	IN	denotes	at
T3571	16205-16208	DT	denotes	the
T3573	16209-16213	JJ	denotes	late
T3574	16214-16225	JJ	denotes	gestational
T3572	16226-16232	NNS	denotes	stages
T3575	16232-16234	,	denotes	,
T3576	16234-16238	NN	denotes	cell
T3578	16239-16252	NN	denotes	proliferation
T3579	16252-16253	SYM	denotes	/
T3577	16253-16261	NN	denotes	division
T3580	16262-16265	VBD	denotes	was
T3568	16266-16274	VBN	denotes	impaired
T3581	16275-16277	IN	denotes	in
T3582	16278-16286	NN	denotes	knockout
T3583	16287-16294	NNS	denotes	embryos
T3584	16295-16299	IN	denotes	with
T3585	16300-16302	DT	denotes	no
T3586	16303-16311	NN	denotes	increase
T3587	16312-16314	IN	denotes	in
T3588	16315-16319	NN	denotes	cell
T3589	16320-16325	NN	denotes	death
T3590	16326-16331	NNS	denotes	rates
T3591	16331-16332	.	denotes	.
T12044	16343-16351	NNS	denotes	Analyses
T12045	16352-16354	IN	denotes	of
T12046	16355-16359	NN	denotes	cell
T12047	16360-16373	NN	denotes	proliferation
T12048	16373-16375	,	denotes	,
T12049	16375-16379	NN	denotes	cell
T12050	16380-16388	NN	denotes	division
T12051	16389-16392	CC	denotes	and
T12052	16393-16397	NN	denotes	cell
T12053	16398-16403	NN	denotes	death
T12054	16403-16404	.	denotes	.
T12055	16404-16650	sentence	denotes	Cell proliferation (PCNA: panels A, B, E and F; BrdU: panels C, D, G and H), cell division (pHH3: panels K, L, O and P) and cell death (TUNEL: panels M and N) were analyzed by immunostaining on lung sections at early and late gestational stages.
T12056	16405-16409	NN	denotes	Cell
T12057	16410-16423	NN	denotes	proliferation
T12059	16424-16425	-LRB-	denotes	(
T12061	16425-16429	NN	denotes	PCNA
T12063	16429-16431	:	denotes	:
T12064	16431-16437	NNS	denotes	panels
T12062	16438-16439	NN	denotes	A
T12065	16439-16441	,	denotes	,
T12066	16441-16442	NN	denotes	B
T12067	16442-16444	,	denotes	,
T12068	16444-16445	NN	denotes	E
T12069	16446-16449	CC	denotes	and
T12070	16450-16451	NN	denotes	F
T12071	16451-16452	:	denotes	;
T12072	16453-16457	NN	denotes	BrdU
T12073	16457-16459	:	denotes	:
T12074	16459-16465	NNS	denotes	panels
T12060	16466-16467	NN	denotes	C
T12075	16467-16469	,	denotes	,
T12076	16469-16470	NN	denotes	D
T12077	16470-16472	,	denotes	,
T12078	16472-16473	NN	denotes	G
T12079	16474-16477	CC	denotes	and
T12080	16478-16479	NN	denotes	H
T12081	16479-16480	-RRB-	denotes	)
T12082	16480-16482	,	denotes	,
T12083	16482-16486	NN	denotes	cell
T12084	16487-16495	NN	denotes	division
T12085	16496-16497	-LRB-	denotes	(
T12087	16497-16501	NN	denotes	pHH3
T12088	16501-16503	:	denotes	:
T12089	16503-16509	NNS	denotes	panels
T12086	16510-16511	NN	denotes	K
T12090	16511-16513	,	denotes	,
T12091	16513-16514	NN	denotes	L
T12092	16514-16516	,	denotes	,
T12093	16516-16517	NN	denotes	O
T12094	16518-16521	CC	denotes	and
T12095	16522-16523	NN	denotes	P
T12096	16523-16524	-RRB-	denotes	)
T12097	16525-16528	CC	denotes	and
T12098	16529-16533	NN	denotes	cell
T12099	16534-16539	NN	denotes	death
T12100	16540-16541	-LRB-	denotes	(
T12102	16541-16546	NN	denotes	TUNEL
T12103	16546-16548	:	denotes	:
T12104	16548-16554	NNS	denotes	panels
T12101	16555-16556	NN	denotes	M
T12105	16557-16560	CC	denotes	and
T12106	16561-16562	NN	denotes	N
T12107	16562-16563	-RRB-	denotes	)
T12108	16564-16568	VBD	denotes	were
T12058	16569-16577	VBN	denotes	analyzed
T12109	16578-16580	IN	denotes	by
T12110	16581-16595	NN	denotes	immunostaining
T12111	16596-16598	IN	denotes	on
T12112	16599-16603	NN	denotes	lung
T12113	16604-16612	NNS	denotes	sections
T12114	16613-16615	IN	denotes	at
T12115	16616-16621	JJ	denotes	early
T12117	16622-16625	CC	denotes	and
T12118	16626-16630	JJ	denotes	late
T12119	16631-16642	JJ	denotes	gestational
T12116	16643-16649	NNS	denotes	stages
T12120	16649-16650	.	denotes	.
T12121	16650-16844	sentence	denotes	Both PCNA and BrdU labeled cells in both epithelial and mesenchymal regions were comparable at early stages (E14.5 for PCNA and E13.5 for BrdU) between wild-type (+/+) and knockout (-/-) lungs.
T12122	16651-16655	CC	denotes	Both
T12123	16656-16660	NN	denotes	PCNA
T12125	16661-16664	CC	denotes	and
T12126	16665-16669	NN	denotes	BrdU
T12124	16670-16677	VBN	denotes	labeled
T12127	16678-16683	NNS	denotes	cells
T12129	16684-16686	IN	denotes	in
T12130	16687-16691	CC	denotes	both
T12131	16692-16702	JJ	denotes	epithelial
T12133	16703-16706	CC	denotes	and
T12134	16707-16718	JJ	denotes	mesenchymal
T12132	16719-16726	NNS	denotes	regions
T12128	16727-16731	VBD	denotes	were
T12135	16732-16742	JJ	denotes	comparable
T12136	16743-16745	IN	denotes	at
T12137	16746-16751	JJ	denotes	early
T12138	16752-16758	NNS	denotes	stages
T12139	16759-16760	-LRB-	denotes	(
T12140	16760-16765	NN	denotes	E14.5
T12141	16766-16769	IN	denotes	for
T12142	16770-16774	NN	denotes	PCNA
T12143	16775-16778	CC	denotes	and
T12144	16779-16784	NN	denotes	E13.5
T12145	16785-16788	IN	denotes	for
T12146	16789-16793	NN	denotes	BrdU
T12147	16793-16794	-RRB-	denotes	)
T12148	16795-16802	IN	denotes	between
T12149	16803-16807	JJ	denotes	wild
T12151	16807-16808	HYPH	denotes	-
T12150	16808-16812	NN	denotes	type
T12153	16813-16814	-LRB-	denotes	(
T12154	16814-16815	SYM	denotes	+
T12156	16815-16816	SYM	denotes	/
T12155	16816-16817	SYM	denotes	+
T12157	16817-16818	-RRB-	denotes	)
T12158	16819-16822	CC	denotes	and
T12159	16823-16831	NN	denotes	knockout
T12160	16832-16833	-LRB-	denotes	(
T12161	16833-16834	SYM	denotes	-
T12163	16834-16835	SYM	denotes	/
T12162	16835-16836	SYM	denotes	-
T12164	16836-16837	-RRB-	denotes	)
T12152	16838-16843	NNS	denotes	lungs
T12165	16843-16844	.	denotes	.
T12166	16844-17003	sentence	denotes	Positively stained cells in distal lungs at late stage (E20.5 for PCNA and E18.5 for BrdU) from knockout lungs were markedly reduced, compared with wild-type.
T12167	16845-16855	RB	denotes	Positively
T12168	16856-16863	VBN	denotes	stained
T12169	16864-16869	NNS	denotes	cells
T12171	16870-16872	IN	denotes	in
T12172	16873-16879	JJ	denotes	distal
T12173	16880-16885	NNS	denotes	lungs
T12174	16886-16888	IN	denotes	at
T12175	16889-16893	JJ	denotes	late
T12176	16894-16899	NN	denotes	stage
T12177	16900-16901	-LRB-	denotes	(
T12178	16901-16906	NN	denotes	E20.5
T12179	16907-16910	IN	denotes	for
T12180	16911-16915	NN	denotes	PCNA
T12181	16916-16919	CC	denotes	and
T12182	16920-16925	NN	denotes	E18.5
T12183	16926-16929	IN	denotes	for
T12184	16930-16934	NN	denotes	BrdU
T12185	16934-16935	-RRB-	denotes	)
T12186	16936-16940	IN	denotes	from
T12187	16941-16949	NN	denotes	knockout
T12188	16950-16955	NNS	denotes	lungs
T12189	16956-16960	VBD	denotes	were
T12190	16961-16969	RB	denotes	markedly
T12170	16970-16977	VBN	denotes	reduced
T12191	16977-16979	,	denotes	,
T12192	16979-16987	VBN	denotes	compared
T12193	16988-16992	IN	denotes	with
T12194	16993-16997	JJ	denotes	wild
T12196	16997-16998	HYPH	denotes	-
T12195	16998-17002	NN	denotes	type
T12197	17002-17003	.	denotes	.
T12198	17003-17106	sentence	denotes	PCNA labeling of cardiac muscle was also attenuated in knockout embryos at E20.5 (cf. panels I and J).
T12199	17004-17008	NN	denotes	PCNA
T12200	17009-17017	NN	denotes	labeling
T12202	17018-17020	IN	denotes	of
T12203	17021-17028	JJ	denotes	cardiac
T12204	17029-17035	NN	denotes	muscle
T12205	17036-17039	VBD	denotes	was
T12206	17040-17044	RB	denotes	also
T12201	17045-17055	VBN	denotes	attenuated
T12207	17056-17058	IN	denotes	in
T12208	17059-17067	NN	denotes	knockout
T12209	17068-17075	NNS	denotes	embryos
T12210	17076-17078	IN	denotes	at
T12211	17079-17084	NN	denotes	E20.5
T12212	17085-17086	-LRB-	denotes	(
T12214	17086-17089	FW	denotes	cf.
T12215	17090-17096	NNS	denotes	panels
T12213	17097-17098	NN	denotes	I
T12216	17099-17102	CC	denotes	and
T12217	17103-17104	NN	denotes	J
T12218	17104-17105	-RRB-	denotes	)
T12219	17105-17106	.	denotes	.
T12220	17106-17226	sentence	denotes	No apparent differences in TUNEL immunostaining were observed in lung sections at E18.5 between wild-type and knockout.
T12221	17107-17109	DT	denotes	No
T12223	17110-17118	JJ	denotes	apparent
T12222	17119-17130	NNS	denotes	differences
T12225	17131-17133	IN	denotes	in
T12226	17134-17139	NN	denotes	TUNEL
T12227	17140-17154	NN	denotes	immunostaining
T12228	17155-17159	VBD	denotes	were
T12224	17160-17168	VBN	denotes	observed
T12229	17169-17171	IN	denotes	in
T12230	17172-17176	NN	denotes	lung
T12231	17177-17185	NNS	denotes	sections
T12232	17186-17188	IN	denotes	at
T12233	17189-17194	NN	denotes	E18.5
T12234	17195-17202	IN	denotes	between
T12235	17203-17207	JJ	denotes	wild
T12237	17207-17208	HYPH	denotes	-
T12236	17208-17212	NN	denotes	type
T12238	17213-17216	CC	denotes	and
T12239	17217-17225	NN	denotes	knockout
T12240	17225-17226	.	denotes	.
T12241	17226-17461	sentence	denotes	The pHH3 immunostaining was comparable between wild-type and knockout lungs at E14.5, and the lungs from wild-type at E20.5 showed a high index of positive cells, whereas lungs from knockout showed a apparent reduced staining pattern.
T12242	17227-17230	DT	denotes	The
T12244	17231-17235	NN	denotes	pHH3
T12243	17236-17250	NN	denotes	immunostaining
T12245	17251-17254	VBD	denotes	was
T12246	17255-17265	JJ	denotes	comparable
T12247	17266-17273	IN	denotes	between
T12248	17274-17278	JJ	denotes	wild
T12250	17278-17279	HYPH	denotes	-
T12249	17279-17283	NN	denotes	type
T12252	17284-17287	CC	denotes	and
T12253	17288-17296	NN	denotes	knockout
T12251	17297-17302	NNS	denotes	lungs
T12254	17303-17305	IN	denotes	at
T12255	17306-17311	NN	denotes	E14.5
T12256	17311-17313	,	denotes	,
T12257	17313-17316	CC	denotes	and
T12258	17317-17320	DT	denotes	the
T12259	17321-17326	NNS	denotes	lungs
T12261	17327-17331	IN	denotes	from
T12262	17332-17336	JJ	denotes	wild
T12264	17336-17337	HYPH	denotes	-
T12263	17337-17341	NN	denotes	type
T12265	17342-17344	IN	denotes	at
T12266	17345-17350	NN	denotes	E20.5
T12260	17351-17357	VBD	denotes	showed
T12267	17358-17359	DT	denotes	a
T12269	17360-17364	JJ	denotes	high
T12268	17365-17370	NN	denotes	index
T12270	17371-17373	IN	denotes	of
T12271	17374-17382	JJ	denotes	positive
T12272	17383-17388	NNS	denotes	cells
T12273	17388-17390	,	denotes	,
T12274	17390-17397	IN	denotes	whereas
T12276	17398-17403	NNS	denotes	lungs
T12277	17404-17408	IN	denotes	from
T12278	17409-17417	NN	denotes	knockout
T12275	17418-17424	VBD	denotes	showed
T12279	17425-17426	DT	denotes	a
T12281	17427-17435	JJ	denotes	apparent
T12282	17436-17443	VBN	denotes	reduced
T12283	17444-17452	NN	denotes	staining
T12280	17453-17460	NN	denotes	pattern
T12284	17460-17461	.	denotes	.
T12285	17461-17508	sentence	denotes	Note the dividing cells are mainly epithelial.
T12286	17462-17466	VB	denotes	Note
T12287	17467-17470	DT	denotes	the
T12289	17471-17479	VBG	denotes	dividing
T12288	17480-17485	NNS	denotes	cells
T12290	17486-17489	VBP	denotes	are
T12291	17490-17496	RB	denotes	mainly
T12292	17497-17507	JJ	denotes	epithelial
T12293	17507-17508	.	denotes	.
T12294	17508-17576	sentence	denotes	Original magnification: A-D and G-N are 20X; E, F, O and P are 40X.
T12295	17509-17517	JJ	denotes	Original
T12296	17518-17531	NN	denotes	magnification
T12298	17531-17533	:	denotes	:
T12299	17533-17534	NN	denotes	A
T12301	17534-17535	SYM	denotes	-
T12302	17535-17536	NN	denotes	D
T12303	17537-17540	CC	denotes	and
T12304	17541-17542	NN	denotes	G
T12305	17542-17543	SYM	denotes	-
T12306	17543-17544	NN	denotes	N
T12300	17545-17548	VBP	denotes	are
T12307	17549-17552	NN	denotes	20X
T12308	17552-17553	:	denotes	;
T12309	17554-17555	NN	denotes	E
T12310	17555-17557	,	denotes	,
T12311	17557-17558	NN	denotes	F
T12312	17558-17560	,	denotes	,
T12313	17560-17561	NN	denotes	O
T12314	17562-17565	CC	denotes	and
T12315	17566-17567	NN	denotes	P
T12297	17568-17571	VBP	denotes	are
T12316	17572-17575	NN	denotes	40X
T12317	17575-17576	.	denotes	.
T3855	17578-17584	NN	denotes	Sterol
T3856	17585-17592	NN	denotes	content
T3857	17593-17595	IN	denotes	of
T3858	17596-17606	VBG	denotes	developing
T3859	17607-17612	NNS	denotes	lungs
T3860	17613-17615	IN	denotes	in
T3861	17616-17621	NN	denotes	Dhcr7
T3862	17622-17631	JJ	denotes	deficient
T3863	17632-17636	NNS	denotes	mice
T3864	17636-17768	sentence	denotes	Markedly elevated 7-DHC/8-DHC levels and reduced body cholesterol levels have been reported previously in Dhcr7-/- P0 mice [16,17].
T3865	17637-17645	RB	denotes	Markedly
T3866	17646-17654	JJ	denotes	elevated
T3868	17655-17656	CD	denotes	7
T3870	17656-17657	HYPH	denotes	-
T3869	17657-17660	NN	denotes	DHC
T3871	17660-17661	SYM	denotes	/
T3872	17661-17662	CD	denotes	8
T3874	17662-17663	HYPH	denotes	-
T3873	17663-17666	NN	denotes	DHC
T3867	17667-17673	NNS	denotes	levels
T3876	17674-17677	CC	denotes	and
T3877	17678-17685	VBN	denotes	reduced
T3879	17686-17690	NN	denotes	body
T3880	17691-17702	NN	denotes	cholesterol
T3878	17703-17709	NNS	denotes	levels
T3881	17710-17714	VBP	denotes	have
T3882	17715-17719	VBN	denotes	been
T3875	17720-17728	VBN	denotes	reported
T3883	17729-17739	RB	denotes	previously
T3884	17740-17742	IN	denotes	in
T3885	17743-17748	NN	denotes	Dhcr7
T3887	17748-17749	SYM	denotes	-
T3888	17749-17750	SYM	denotes	/
T3889	17750-17751	SYM	denotes	-
T3890	17752-17754	NN	denotes	P0
T3886	17755-17759	NNS	denotes	mice
T3891	17760-17761	-LRB-	denotes	[
T3893	17761-17763	CD	denotes	16
T3894	17763-17764	,	denotes	,
T3892	17764-17766	CD	denotes	17
T3895	17766-17767	-RRB-	denotes	]
T3896	17767-17768	.	denotes	.
T3897	17768-17931	sentence	denotes	A significant amount of cholesterol was detectable in lungs of Dhcr7-/- mice, comprising ~60% and ~30% of total tissue sterols at E13.5 and neonate, respectively.
T3898	17769-17770	DT	denotes	A
T3900	17771-17782	JJ	denotes	significant
T3899	17783-17789	NN	denotes	amount
T3902	17790-17792	IN	denotes	of
T3903	17793-17804	NN	denotes	cholesterol
T3901	17805-17808	VBD	denotes	was
T3904	17809-17819	JJ	denotes	detectable
T3905	17820-17822	IN	denotes	in
T3906	17823-17828	NNS	denotes	lungs
T3907	17829-17831	IN	denotes	of
T3908	17832-17837	NN	denotes	Dhcr7
T3910	17837-17838	SYM	denotes	-
T3911	17838-17839	SYM	denotes	/
T3912	17839-17840	SYM	denotes	-
T3909	17841-17845	NNS	denotes	mice
T3913	17845-17847	,	denotes	,
T3914	17847-17857	VBG	denotes	comprising
T3915	17858-17859	SYM	denotes	~
T3916	17859-17861	CD	denotes	60
T3917	17861-17862	NN	denotes	%
T3918	17863-17866	CC	denotes	and
T3919	17867-17868	SYM	denotes	~
T3920	17868-17870	CD	denotes	30
T3921	17870-17871	NN	denotes	%
T3922	17872-17874	IN	denotes	of
T3923	17875-17880	JJ	denotes	total
T3925	17881-17887	NN	denotes	tissue
T3924	17888-17895	NNS	denotes	sterols
T3926	17896-17898	IN	denotes	at
T3927	17899-17904	NN	denotes	E13.5
T3928	17905-17908	CC	denotes	and
T3929	17909-17916	NN	denotes	neonate
T3930	17916-17918	,	denotes	,
T3931	17918-17930	RB	denotes	respectively
T3932	17930-17931	.	denotes	.
T3933	17931-18207	sentence	denotes	In contrast to the doubling of cholesterol in the lungs of wild type embryos (1.82 mg/g tissue at E13.5 to 3.55 mg/g tissue at P0), cholesterol content in the lungs of Dhcr7 null embryos remained at almost constant low levels between E13.5 and P0 gestational stages (Fig. 5).
T3934	17932-17934	IN	denotes	In
T3936	17935-17943	NN	denotes	contrast
T3937	17944-17946	IN	denotes	to
T3938	17947-17950	DT	denotes	the
T3939	17951-17959	NN	denotes	doubling
T3940	17960-17962	IN	denotes	of
T3941	17963-17974	NN	denotes	cholesterol
T3942	17975-17977	IN	denotes	in
T3943	17978-17981	DT	denotes	the
T3944	17982-17987	NNS	denotes	lungs
T3945	17988-17990	IN	denotes	of
T3946	17991-17995	JJ	denotes	wild
T3947	17996-18000	NN	denotes	type
T3948	18001-18008	NNS	denotes	embryos
T3949	18009-18010	-LRB-	denotes	(
T3951	18010-18014	CD	denotes	1.82
T3950	18015-18017	NNS	denotes	mg
T3952	18017-18018	SYM	denotes	/
T3954	18018-18019	NN	denotes	g
T3953	18020-18026	NN	denotes	tissue
T3955	18027-18029	IN	denotes	at
T3956	18030-18035	NN	denotes	E13.5
T3957	18036-18038	IN	denotes	to
T3958	18039-18043	CD	denotes	3.55
T3959	18044-18046	NNS	denotes	mg
T3960	18046-18047	SYM	denotes	/
T3962	18047-18048	NN	denotes	g
T3961	18049-18055	NN	denotes	tissue
T3963	18056-18058	IN	denotes	at
T3964	18059-18061	NN	denotes	P0
T3965	18061-18062	-RRB-	denotes	)
T3966	18062-18064	,	denotes	,
T3967	18064-18075	NN	denotes	cholesterol
T3968	18076-18083	NN	denotes	content
T3969	18084-18086	IN	denotes	in
T3970	18087-18090	DT	denotes	the
T3971	18091-18096	NNS	denotes	lungs
T3972	18097-18099	IN	denotes	of
T3973	18100-18105	NN	denotes	Dhcr7
T3974	18106-18110	JJ	denotes	null
T3975	18111-18118	NNS	denotes	embryos
T3935	18119-18127	VBD	denotes	remained
T3976	18128-18130	IN	denotes	at
T3977	18131-18137	RB	denotes	almost
T3978	18138-18146	JJ	denotes	constant
T3980	18147-18150	JJ	denotes	low
T3979	18151-18157	NNS	denotes	levels
T3981	18158-18165	IN	denotes	between
T3982	18166-18171	NN	denotes	E13.5
T3984	18172-18175	CC	denotes	and
T3985	18176-18178	NN	denotes	P0
T3986	18179-18190	JJ	denotes	gestational
T3983	18191-18197	NNS	denotes	stages
T3987	18198-18199	-LRB-	denotes	(
T3988	18199-18203	NN	denotes	Fig.
T3989	18204-18205	CD	denotes	5
T3990	18205-18206	-RRB-	denotes	)
T3991	18206-18207	.	denotes	.
T3992	18207-18332	sentence	denotes	There was a progressive increase in 7-DHC and 8-DHC; the latter is generated as a spontaneous isomerization from the former.
T3993	18208-18213	EX	denotes	There
T3994	18214-18217	VBD	denotes	was
T3996	18218-18219	DT	denotes	a
T3998	18220-18231	JJ	denotes	progressive
T3997	18232-18240	NN	denotes	increase
T3999	18241-18243	IN	denotes	in
T4000	18244-18245	CD	denotes	7
T4002	18245-18246	HYPH	denotes	-
T4001	18246-18249	NN	denotes	DHC
T4003	18250-18253	CC	denotes	and
T4004	18254-18255	CD	denotes	8
T4006	18255-18256	HYPH	denotes	-
T4005	18256-18259	NN	denotes	DHC
T4007	18259-18260	:	denotes	;
T4008	18261-18264	DT	denotes	the
T4009	18265-18271	JJ	denotes	latter
T4010	18272-18274	VBZ	denotes	is
T3995	18275-18284	VBN	denotes	generated
T4011	18285-18287	IN	denotes	as
T4012	18288-18289	DT	denotes	a
T4014	18290-18301	JJ	denotes	spontaneous
T4013	18302-18315	NN	denotes	isomerization
T4015	18316-18320	IN	denotes	from
T4016	18321-18324	DT	denotes	the
T4017	18325-18331	JJ	denotes	former
T4018	18331-18332	.	denotes	.
T4019	18332-18463	sentence	denotes	Total sterols at birth were reduced in lungs of the Dhcr7 null mice, but were only lower by ~30% (Fig. 5, see values for P0 mice).
T4020	18333-18338	JJ	denotes	Total
T4021	18339-18346	NNS	denotes	sterols
T4023	18347-18349	IN	denotes	at
T4024	18350-18355	NN	denotes	birth
T4025	18356-18360	VBD	denotes	were
T4022	18361-18368	VBN	denotes	reduced
T4026	18369-18371	IN	denotes	in
T4027	18372-18377	NNS	denotes	lungs
T4028	18378-18380	IN	denotes	of
T4029	18381-18384	DT	denotes	the
T4031	18385-18390	NN	denotes	Dhcr7
T4032	18391-18395	JJ	denotes	null
T4030	18396-18400	NNS	denotes	mice
T4033	18400-18402	,	denotes	,
T4034	18402-18405	CC	denotes	but
T4035	18406-18410	VBD	denotes	were
T4036	18411-18415	RB	denotes	only
T4037	18416-18421	JJR	denotes	lower
T4038	18422-18424	IN	denotes	by
T4039	18425-18426	SYM	denotes	~
T4040	18426-18428	CD	denotes	30
T4041	18428-18429	NN	denotes	%
T4042	18430-18431	-LRB-	denotes	(
T4043	18431-18435	NN	denotes	Fig.
T4044	18436-18437	CD	denotes	5
T4045	18437-18439	,	denotes	,
T4046	18439-18442	VB	denotes	see
T4047	18443-18449	NNS	denotes	values
T4048	18450-18453	IN	denotes	for
T4049	18454-18456	NN	denotes	P0
T4050	18457-18461	NNS	denotes	mice
T4051	18461-18462	-RRB-	denotes	)
T4052	18462-18463	.	denotes	.
T4053	18463-18464	sentence	denotes
T12360	18474-18480	NN	denotes	Sterol
T12361	18481-18489	NNS	denotes	analyses
T12362	18490-18492	IN	denotes	of
T12363	18493-18502	JJ	denotes	embryonic
T12364	18503-18508	NNS	denotes	lungs
T12365	18508-18509	.	denotes	.
T12366	18509-18677	sentence	denotes	Sterol contents, including cholesterol (black bars) and 7DHC/8DHC (white bars), were quantitated by GC-MS in the lungs from wild-type (+/+) and knockout (-/-) embryos.
T12367	18510-18516	NN	denotes	Sterol
T12368	18517-18525	NNS	denotes	contents
T12370	18525-18527	,	denotes	,
T12371	18527-18536	VBG	denotes	including
T12372	18537-18548	NN	denotes	cholesterol
T12373	18549-18550	-LRB-	denotes	(
T12375	18550-18555	JJ	denotes	black
T12374	18556-18560	NNS	denotes	bars
T12376	18560-18561	-RRB-	denotes	)
T12377	18562-18565	CC	denotes	and
T12378	18566-18570	NN	denotes	7DHC
T12380	18570-18571	SYM	denotes	/
T12379	18571-18575	NN	denotes	8DHC
T12381	18576-18577	-LRB-	denotes	(
T12383	18577-18582	JJ	denotes	white
T12382	18583-18587	NNS	denotes	bars
T12384	18587-18588	-RRB-	denotes	)
T12385	18588-18590	,	denotes	,
T12386	18590-18594	VBD	denotes	were
T12369	18595-18606	VBN	denotes	quantitated
T12387	18607-18609	IN	denotes	by
T12388	18610-18612	NN	denotes	GC
T12390	18612-18613	HYPH	denotes	-
T12389	18613-18615	NN	denotes	MS
T12391	18616-18618	IN	denotes	in
T12392	18619-18622	DT	denotes	the
T12393	18623-18628	NNS	denotes	lungs
T12394	18629-18633	IN	denotes	from
T12395	18634-18638	JJ	denotes	wild
T12397	18638-18639	HYPH	denotes	-
T12396	18639-18643	NN	denotes	type
T12399	18644-18645	-LRB-	denotes	(
T12400	18645-18646	SYM	denotes	+
T12402	18646-18647	SYM	denotes	/
T12401	18647-18648	SYM	denotes	+
T12403	18648-18649	-RRB-	denotes	)
T12404	18650-18653	CC	denotes	and
T12405	18654-18662	NN	denotes	knockout
T12406	18663-18664	-LRB-	denotes	(
T12407	18664-18665	SYM	denotes	-
T12409	18665-18666	SYM	denotes	/
T12408	18666-18667	SYM	denotes	-
T12410	18667-18668	-RRB-	denotes	)
T12398	18669-18676	NNS	denotes	embryos
T12411	18676-18677	.	denotes	.
T12412	18677-18763	sentence	denotes	Results are expressed as mean ± s.d and the number of lungs analyzed is as indicated.
T12413	18678-18685	NNS	denotes	Results
T12415	18686-18689	VBP	denotes	are
T12414	18690-18699	VBN	denotes	expressed
T12416	18700-18702	IN	denotes	as
T12417	18703-18707	NN	denotes	mean
T12419	18708-18709	SYM	denotes	±
T12418	18710-18713	NN	denotes	s.d
T12420	18714-18717	CC	denotes	and
T12421	18718-18721	DT	denotes	the
T12422	18722-18728	NN	denotes	number
T12424	18729-18731	IN	denotes	of
T12425	18732-18737	NNS	denotes	lungs
T12426	18738-18746	VBN	denotes	analyzed
T12423	18747-18749	VBZ	denotes	is
T12427	18750-18752	IN	denotes	as
T12428	18753-18762	VBN	denotes	indicated
T12429	18762-18763	.	denotes	.
T12430	18763-19006	sentence	denotes	Total sterols were lower at all stages in knockout lungs, with almost no increase in cholesterol content with time (black bars), but there was a progressive increment in the precursor sterols, 7DHC/8DHC (open bars), with increasing gestation.
T12431	18764-18769	JJ	denotes	Total
T12432	18770-18777	NNS	denotes	sterols
T12433	18778-18782	VBD	denotes	were
T12434	18783-18788	JJR	denotes	lower
T12435	18789-18791	IN	denotes	at
T12436	18792-18795	DT	denotes	all
T12437	18796-18802	NNS	denotes	stages
T12438	18803-18805	IN	denotes	in
T12439	18806-18814	NN	denotes	knockout
T12440	18815-18820	NNS	denotes	lungs
T12441	18820-18822	,	denotes	,
T12442	18822-18826	IN	denotes	with
T12443	18827-18833	RB	denotes	almost
T12445	18834-18836	DT	denotes	no
T12444	18837-18845	NN	denotes	increase
T12446	18846-18848	IN	denotes	in
T12447	18849-18860	NN	denotes	cholesterol
T12448	18861-18868	NN	denotes	content
T12449	18869-18873	IN	denotes	with
T12450	18874-18878	NN	denotes	time
T12451	18879-18880	-LRB-	denotes	(
T12453	18880-18885	JJ	denotes	black
T12452	18886-18890	NNS	denotes	bars
T12454	18890-18891	-RRB-	denotes	)
T12455	18891-18893	,	denotes	,
T12456	18893-18896	CC	denotes	but
T12457	18897-18902	EX	denotes	there
T12458	18903-18906	VBD	denotes	was
T12459	18907-18908	DT	denotes	a
T12461	18909-18920	JJ	denotes	progressive
T12460	18921-18930	NN	denotes	increment
T12462	18931-18933	IN	denotes	in
T12463	18934-18937	DT	denotes	the
T12465	18938-18947	NN	denotes	precursor
T12464	18948-18955	NNS	denotes	sterols
T12466	18955-18957	,	denotes	,
T12467	18957-18961	NN	denotes	7DHC
T12469	18961-18962	SYM	denotes	/
T12468	18962-18966	NN	denotes	8DHC
T12470	18967-18968	-LRB-	denotes	(
T12472	18968-18972	JJ	denotes	open
T12471	18973-18977	NNS	denotes	bars
T12473	18977-18978	-RRB-	denotes	)
T12474	18978-18980	,	denotes	,
T12475	18980-18984	IN	denotes	with
T12476	18985-18995	VBG	denotes	increasing
T12477	18996-19005	NN	denotes	gestation
T12478	19005-19006	.	denotes	.
T4055	19007-19008	NN	denotes	C
T4054	19007-19013	sentence	denotes	Choles
T4056	19008-19009	JJ	denotes	h
T4057	19009-19010	IN	denotes	o
T4058	19010-19011	JJ	denotes	l
T4059	19011-19012	NN	denotes	e
T4060	19012-19013	NNS	denotes	s
T4062	19013-19014	NNS	denotes	t
T4061	19013-19023	sentence	denotes	terol is a
T4064	19014-19015	VBP	denotes	e
T4063	19015-19016	VBN	denotes	r
T4066	19016-19017	IN	denotes	o
T4067	19017-19018	NNS	denotes	l
T4065	19019-19021	VBZ	denotes	is
T4068	19022-19023	IN	denotes	a
T4071	19023-19024	JJ	denotes	b
T4069	19023-19027	sentence	denotes	bsol
T4070	19024-19025	NNS	denotes	s
T4072	19025-19026	JJ	denotes	o
T4073	19026-19027	NNS	denotes	l
T4076	19027-19032	RB	denotes	utely
T4074	19027-19094	sentence	denotes	utely required for steroid hormone synthesis by the adrenal glands.
T4075	19033-19041	VBN	denotes	required
T4077	19042-19045	IN	denotes	for
T4078	19046-19053	NN	denotes	steroid
T4079	19054-19061	NN	denotes	hormone
T4080	19062-19071	NN	denotes	synthesis
T4081	19072-19074	IN	denotes	by
T4082	19075-19078	DT	denotes	the
T4084	19079-19086	JJ	denotes	adrenal
T4083	19087-19093	NNS	denotes	glands
T4085	19093-19094	.	denotes	.
T4086	19094-19252	sentence	denotes	Adrenal insufficiency, as has been reported in some severe cases of SLOS patients, could be responsible for the lung hypoplasia and perinatal lethality [30].
T4087	19095-19102	JJ	denotes	Adrenal
T4088	19103-19116	NN	denotes	insufficiency
T4090	19116-19118	,	denotes	,
T4091	19118-19120	IN	denotes	as
T4093	19121-19124	VBZ	denotes	has
T4094	19125-19129	VBN	denotes	been
T4092	19130-19138	VBN	denotes	reported
T4095	19139-19141	IN	denotes	in
T4096	19142-19146	DT	denotes	some
T4098	19147-19153	JJ	denotes	severe
T4097	19154-19159	NNS	denotes	cases
T4099	19160-19162	IN	denotes	of
T4100	19163-19167	NN	denotes	SLOS
T4101	19168-19176	NNS	denotes	patients
T4102	19176-19178	,	denotes	,
T4103	19178-19183	MD	denotes	could
T4089	19184-19186	VB	denotes	be
T4104	19187-19198	JJ	denotes	responsible
T4105	19199-19202	IN	denotes	for
T4106	19203-19206	DT	denotes	the
T4108	19207-19211	NN	denotes	lung
T4107	19212-19222	NN	denotes	hypoplasia
T4109	19223-19226	CC	denotes	and
T4110	19227-19236	JJ	denotes	perinatal
T4111	19237-19246	NN	denotes	lethality
T4112	19247-19248	-LRB-	denotes	[
T4113	19248-19250	CD	denotes	30
T4114	19250-19251	-RRB-	denotes	]
T4115	19251-19252	.	denotes	.
T4116	19252-19401	sentence	denotes	Steroids, but particularly corticosteroids, are necessary for lung maturation, especially the synthesis and secretion of surfactant components [31].
T4117	19253-19261	NNS	denotes	Steroids
T4119	19261-19263	,	denotes	,
T4120	19263-19266	CC	denotes	but
T4121	19267-19279	RB	denotes	particularly
T4122	19280-19295	NNS	denotes	corticosteroids
T4123	19295-19297	,	denotes	,
T4118	19297-19300	VBP	denotes	are
T4124	19301-19310	JJ	denotes	necessary
T4125	19311-19314	IN	denotes	for
T4126	19315-19319	NN	denotes	lung
T4127	19320-19330	NN	denotes	maturation
T4128	19330-19332	,	denotes	,
T4129	19332-19342	RB	denotes	especially
T4131	19343-19346	DT	denotes	the
T4130	19347-19356	NN	denotes	synthesis
T4132	19357-19360	CC	denotes	and
T4133	19361-19370	NN	denotes	secretion
T4134	19371-19373	IN	denotes	of
T4135	19374-19384	NN	denotes	surfactant
T4136	19385-19395	NNS	denotes	components
T4137	19396-19397	-LRB-	denotes	[
T4138	19397-19399	CD	denotes	31
T4139	19399-19400	-RRB-	denotes	]
T4140	19400-19401	.	denotes	.
T4141	19401-19477	sentence	denotes	We determined plasma corticosterone levels in P0 mice within 12 h of birth.
T4142	19402-19404	PRP	denotes	We
T4143	19405-19415	VBD	denotes	determined
T4144	19416-19422	NN	denotes	plasma
T4145	19423-19437	NN	denotes	corticosterone
T4146	19438-19444	NNS	denotes	levels
T4147	19445-19447	IN	denotes	in
T4148	19448-19450	NN	denotes	P0
T4149	19451-19455	NNS	denotes	mice
T4150	19456-19462	IN	denotes	within
T4151	19463-19465	CD	denotes	12
T4152	19466-19467	NN	denotes	h
T4153	19468-19470	IN	denotes	of
T4154	19471-19476	NN	denotes	birth
T4155	19476-19477	.	denotes	.
T4156	19477-19617	sentence	denotes	Wild type P0 mice had corticosterone levels of 158 ± 50 ng/ml (n = 11), compared with 225 ± 50 ng/ml (n = 7, p < 0.01) in Dhcr7-/- P0 mice.
T4157	19478-19482	JJ	denotes	Wild
T4158	19483-19487	NN	denotes	type
T4160	19488-19490	NN	denotes	P0
T4159	19491-19495	NNS	denotes	mice
T4161	19496-19499	VBD	denotes	had
T4162	19500-19514	NN	denotes	corticosterone
T4163	19515-19521	NNS	denotes	levels
T4164	19522-19524	IN	denotes	of
T4165	19525-19528	CD	denotes	158
T4167	19529-19530	SYM	denotes	±
T4166	19531-19533	CD	denotes	50
T4168	19534-19536	NNS	denotes	ng
T4169	19536-19537	SYM	denotes	/
T4170	19537-19539	NN	denotes	ml
T4171	19540-19541	-LRB-	denotes	(
T4173	19541-19542	NN	denotes	n
T4174	19543-19544	SYM	denotes	=
T4172	19545-19547	CD	denotes	11
T4175	19547-19548	-RRB-	denotes	)
T4176	19548-19550	,	denotes	,
T4177	19550-19558	VBN	denotes	compared
T4178	19559-19563	IN	denotes	with
T4179	19564-19567	CD	denotes	225
T4181	19568-19569	SYM	denotes	±
T4180	19570-19572	CD	denotes	50
T4182	19573-19575	NNS	denotes	ng
T4183	19575-19576	SYM	denotes	/
T4184	19576-19578	NN	denotes	ml
T4185	19579-19580	-LRB-	denotes	(
T4187	19580-19581	NN	denotes	n
T4189	19582-19583	SYM	denotes	=
T4188	19584-19585	CD	denotes	7
T4190	19585-19587	,	denotes	,
T4191	19587-19588	NN	denotes	p
T4192	19589-19590	SYM	denotes	<
T4186	19591-19595	CD	denotes	0.01
T4193	19595-19596	-RRB-	denotes	)
T4194	19597-19599	IN	denotes	in
T4195	19600-19605	NN	denotes	Dhcr7
T4197	19605-19606	SYM	denotes	-
T4198	19606-19607	SYM	denotes	/
T4199	19607-19608	SYM	denotes	-
T4200	19609-19611	NN	denotes	P0
T4196	19612-19616	NNS	denotes	mice
T4201	19616-19617	.	denotes	.
T4202	19617-19709	sentence	denotes	Thus, the corticosterone levels in Dhcr7-/- mice were not decreased, but increased by ~40%.
T4203	19618-19622	RB	denotes	Thus
T4205	19622-19624	,	denotes	,
T4206	19624-19627	DT	denotes	the
T4208	19628-19642	NN	denotes	corticosterone
T4207	19643-19649	NNS	denotes	levels
T4209	19650-19652	IN	denotes	in
T4210	19653-19658	NN	denotes	Dhcr7
T4212	19658-19659	SYM	denotes	-
T4213	19659-19660	SYM	denotes	/
T4214	19660-19661	SYM	denotes	-
T4211	19662-19666	NNS	denotes	mice
T4215	19667-19671	VBD	denotes	were
T4216	19672-19675	RB	denotes	not
T4204	19676-19685	VBN	denotes	decreased
T4217	19685-19687	,	denotes	,
T4218	19687-19690	CC	denotes	but
T4219	19691-19700	VBN	denotes	increased
T4220	19701-19703	IN	denotes	by
T4221	19704-19705	SYM	denotes	~
T4222	19705-19707	CD	denotes	40
T4223	19707-19708	NN	denotes	%
T4224	19708-19709	.	denotes	.
T4225	19709-19806	sentence	denotes	However, since these P0 mice were dying, perhaps this increase was sub-optimal given the stress.
T4226	19710-19717	RB	denotes	However
T4228	19717-19719	,	denotes	,
T4229	19719-19724	IN	denotes	since
T4231	19725-19730	DT	denotes	these
T4233	19731-19733	NN	denotes	P0
T4232	19734-19738	NNS	denotes	mice
T4234	19739-19743	VBD	denotes	were
T4230	19744-19749	VBG	denotes	dying
T4235	19749-19751	,	denotes	,
T4236	19751-19758	RB	denotes	perhaps
T4237	19759-19763	DT	denotes	this
T4238	19764-19772	NN	denotes	increase
T4227	19773-19776	VBD	denotes	was
T4239	19777-19788	JJ	denotes	sub-optimal
T4240	19789-19794	VBN	denotes	given
T4241	19795-19798	DT	denotes	the
T4242	19799-19805	NN	denotes	stress
T4243	19805-19806	.	denotes	.
T4244	19806-19895	sentence	denotes	Nevertheless, an absolute lack of corticosterone was not present in these knockout pups.
T4245	19807-19819	RB	denotes	Nevertheless
T4247	19819-19821	,	denotes	,
T4248	19821-19823	DT	denotes	an
T4250	19824-19832	JJ	denotes	absolute
T4249	19833-19837	NN	denotes	lack
T4251	19838-19840	IN	denotes	of
T4252	19841-19855	NN	denotes	corticosterone
T4246	19856-19859	VBD	denotes	was
T4253	19860-19863	RB	denotes	not
T4254	19864-19871	JJ	denotes	present
T4255	19872-19874	IN	denotes	in
T4256	19875-19880	DT	denotes	these
T4258	19881-19889	NN	denotes	knockout
T4257	19890-19894	NNS	denotes	pups
T4259	19894-19895	.	denotes	.
T4260	19895-20029	sentence	denotes	To further rule out any effects of corticosterone deficiency on lung immaturity, we examined lungs for surfactant protein expression.
T4261	19896-19898	TO	denotes	To
T4263	19899-19906	RB	denotes	further
T4262	19907-19911	VB	denotes	rule
T4265	19912-19915	RP	denotes	out
T4266	19916-19919	DT	denotes	any
T4267	19920-19927	NNS	denotes	effects
T4268	19928-19930	IN	denotes	of
T4269	19931-19945	NN	denotes	corticosterone
T4270	19946-19956	NN	denotes	deficiency
T4271	19957-19959	IN	denotes	on
T4272	19960-19964	NN	denotes	lung
T4273	19965-19975	NN	denotes	immaturity
T4274	19975-19977	,	denotes	,
T4275	19977-19979	PRP	denotes	we
T4264	19980-19988	VBD	denotes	examined
T4276	19989-19994	NNS	denotes	lungs
T4277	19995-19998	IN	denotes	for
T4278	19999-20009	NN	denotes	surfactant
T4279	20010-20017	NN	denotes	protein
T4280	20018-20028	NN	denotes	expression
T4281	20028-20029	.	denotes	.
T4282	20029-20206	sentence	denotes	Surfactant protein C, as determined by IHC, was comparable in lung sections from wild-type or Dhcr7 null embryos at either E18.5 or at E20.5 gestational ages (Fig. 6, panel A).
T4283	20030-20040	NN	denotes	Surfactant
T4285	20041-20048	NN	denotes	protein
T4284	20049-20050	NN	denotes	C
T4287	20050-20052	,	denotes	,
T4288	20052-20054	IN	denotes	as
T4289	20055-20065	VBN	denotes	determined
T4290	20066-20068	IN	denotes	by
T4291	20069-20072	NN	denotes	IHC
T4292	20072-20074	,	denotes	,
T4286	20074-20077	VBD	denotes	was
T4293	20078-20088	JJ	denotes	comparable
T4294	20089-20091	IN	denotes	in
T4295	20092-20096	NN	denotes	lung
T4296	20097-20105	NNS	denotes	sections
T4297	20106-20110	IN	denotes	from
T4298	20111-20115	JJ	denotes	wild
T4300	20115-20116	HYPH	denotes	-
T4299	20116-20120	NN	denotes	type
T4302	20121-20123	CC	denotes	or
T4303	20124-20129	NN	denotes	Dhcr7
T4304	20130-20134	JJ	denotes	null
T4301	20135-20142	NNS	denotes	embryos
T4305	20143-20145	IN	denotes	at
T4306	20146-20152	CC	denotes	either
T4307	20153-20158	NN	denotes	E18.5
T4308	20159-20161	CC	denotes	or
T4309	20162-20164	IN	denotes	at
T4310	20165-20170	NN	denotes	E20.5
T4311	20171-20182	JJ	denotes	gestational
T4312	20183-20187	NNS	denotes	ages
T4313	20188-20189	-LRB-	denotes	(
T4315	20189-20193	NN	denotes	Fig.
T4316	20194-20195	CD	denotes	6
T4317	20195-20197	,	denotes	,
T4318	20197-20202	NN	denotes	panel
T4314	20203-20204	NN	denotes	A
T4319	20204-20205	-RRB-	denotes	)
T4320	20205-20206	.	denotes	.
T4321	20206-20354	sentence	denotes	Northern analyses for SP-A, SP-B, SP-C or SP-D showed no significantly differences between wild-type and Dhcr7 null mice at term (Fig. 6, panel B).
T4322	20207-20215	NNP	denotes	Northern
T4323	20216-20224	NNS	denotes	analyses
T4325	20225-20228	IN	denotes	for
T4326	20229-20231	NN	denotes	SP
T4328	20231-20232	HYPH	denotes	-
T4327	20232-20233	NN	denotes	A
T4329	20233-20235	,	denotes	,
T4330	20235-20237	NN	denotes	SP
T4332	20237-20238	HYPH	denotes	-
T4331	20238-20239	NN	denotes	B
T4333	20239-20241	,	denotes	,
T4334	20241-20243	NN	denotes	SP
T4336	20243-20244	HYPH	denotes	-
T4335	20244-20245	NN	denotes	C
T4337	20246-20248	CC	denotes	or
T4338	20249-20251	NN	denotes	SP
T4340	20251-20252	HYPH	denotes	-
T4339	20252-20253	NN	denotes	D
T4324	20254-20260	VBD	denotes	showed
T4341	20261-20263	DT	denotes	no
T4343	20264-20277	RB	denotes	significantly
T4342	20278-20289	NNS	denotes	differences
T4344	20290-20297	IN	denotes	between
T4345	20298-20302	JJ	denotes	wild
T4347	20302-20303	HYPH	denotes	-
T4346	20303-20307	NN	denotes	type
T4349	20308-20311	CC	denotes	and
T4350	20312-20317	NN	denotes	Dhcr7
T4351	20318-20322	JJ	denotes	null
T4348	20323-20327	NNS	denotes	mice
T4352	20328-20330	IN	denotes	at
T4353	20331-20335	NN	denotes	term
T4354	20336-20337	-LRB-	denotes	(
T4356	20337-20341	NN	denotes	Fig.
T4357	20342-20343	CD	denotes	6
T4358	20343-20345	,	denotes	,
T4359	20345-20350	NN	denotes	panel
T4355	20351-20352	NN	denotes	B
T4360	20352-20353	-RRB-	denotes	)
T4361	20353-20354	.	denotes	.
T4362	20354-20427	sentence	denotes	Thus, surfactant protein expression was not altered by Dhcr7 deficiency.
T4363	20355-20359	RB	denotes	Thus
T4365	20359-20361	,	denotes	,
T4366	20361-20371	NN	denotes	surfactant
T4367	20372-20379	NN	denotes	protein
T4368	20380-20390	NN	denotes	expression
T4369	20391-20394	VBD	denotes	was
T4370	20395-20398	RB	denotes	not
T4364	20399-20406	VBN	denotes	altered
T4371	20407-20409	IN	denotes	by
T4372	20410-20415	NN	denotes	Dhcr7
T4373	20416-20426	NN	denotes	deficiency
T4374	20426-20427	.	denotes	.
T12551	20438-20448	NN	denotes	Surfactant
T12552	20449-20456	NN	denotes	protein
T12553	20457-20467	NN	denotes	expression
T12554	20468-20470	IN	denotes	in
T12555	20471-20476	NNS	denotes	lungs
T12556	20476-20477	.	denotes	.
T12557	20477-20605	sentence	denotes	Panel A shows the immunostaining patterns for SP-C in lungs at E18.5 and E20.5 from wild-type (+/+) and knockout (-/-) embryos.
T12558	20478-20483	NN	denotes	Panel
T12559	20484-20485	NN	denotes	A
T12560	20486-20491	VBZ	denotes	shows
T12561	20492-20495	DT	denotes	the
T12563	20496-20510	NN	denotes	immunostaining
T12562	20511-20519	NNS	denotes	patterns
T12564	20520-20523	IN	denotes	for
T12565	20524-20526	NN	denotes	SP
T12567	20526-20527	HYPH	denotes	-
T12566	20527-20528	NN	denotes	C
T12568	20529-20531	IN	denotes	in
T12569	20532-20537	NNS	denotes	lungs
T12570	20538-20540	IN	denotes	at
T12571	20541-20546	NN	denotes	E18.5
T12572	20547-20550	CC	denotes	and
T12573	20551-20556	NN	denotes	E20.5
T12574	20557-20561	IN	denotes	from
T12575	20562-20566	JJ	denotes	wild
T12577	20566-20567	HYPH	denotes	-
T12576	20567-20571	NN	denotes	type
T12579	20572-20573	-LRB-	denotes	(
T12580	20573-20574	SYM	denotes	+
T12582	20574-20575	SYM	denotes	/
T12581	20575-20576	SYM	denotes	+
T12583	20576-20577	-RRB-	denotes	)
T12584	20578-20581	CC	denotes	and
T12585	20582-20590	NN	denotes	knockout
T12586	20591-20592	-LRB-	denotes	(
T12587	20592-20593	SYM	denotes	-
T12589	20593-20594	SYM	denotes	/
T12588	20594-20595	SYM	denotes	-
T12590	20595-20596	-RRB-	denotes	)
T12578	20597-20604	NNS	denotes	embryos
T12591	20604-20605	.	denotes	.
T12592	20605-20731	sentence	denotes	No significant differences in the patterns of SP-C staining were evident between wild-type and knockout lungs (panel A, a-d).
T12593	20606-20608	DT	denotes	No
T12595	20609-20620	JJ	denotes	significant
T12594	20621-20632	NNS	denotes	differences
T12597	20633-20635	IN	denotes	in
T12598	20636-20639	DT	denotes	the
T12599	20640-20648	NNS	denotes	patterns
T12600	20649-20651	IN	denotes	of
T12601	20652-20654	NN	denotes	SP
T12603	20654-20655	HYPH	denotes	-
T12602	20655-20656	NN	denotes	C
T12604	20657-20665	NN	denotes	staining
T12596	20666-20670	VBD	denotes	were
T12605	20671-20678	JJ	denotes	evident
T12606	20679-20686	IN	denotes	between
T12607	20687-20691	JJ	denotes	wild
T12609	20691-20692	HYPH	denotes	-
T12608	20692-20696	NN	denotes	type
T12611	20697-20700	CC	denotes	and
T12612	20701-20709	NN	denotes	knockout
T12610	20710-20715	NNS	denotes	lungs
T12613	20716-20717	-LRB-	denotes	(
T12615	20717-20722	NN	denotes	panel
T12614	20723-20724	NN	denotes	A
T12616	20724-20726	,	denotes	,
T12617	20726-20727	LS	denotes	a
T12618	20727-20728	HYPH	denotes	-
T12619	20728-20729	LS	denotes	d
T12620	20729-20730	-RRB-	denotes	)
T12621	20730-20731	.	denotes	.
T12622	20731-20897	sentence	denotes	Note that the underdeveloped epithelial tubules in knockout lungs also expressed SP-C (panel A, b and d, arrowheads), indicating normal type II AECs differentiation.
T12623	20732-20736	VB	denotes	Note
T12624	20737-20741	IN	denotes	that
T12626	20742-20745	DT	denotes	the
T12628	20746-20760	JJ	denotes	underdeveloped
T12629	20761-20771	JJ	denotes	epithelial
T12627	20772-20779	NNS	denotes	tubules
T12630	20780-20782	IN	denotes	in
T12631	20783-20791	NN	denotes	knockout
T12632	20792-20797	NNS	denotes	lungs
T12633	20798-20802	RB	denotes	also
T12625	20803-20812	VBD	denotes	expressed
T12634	20813-20815	NN	denotes	SP
T12636	20815-20816	HYPH	denotes	-
T12635	20816-20817	NN	denotes	C
T12637	20818-20819	-LRB-	denotes	(
T12639	20819-20824	NN	denotes	panel
T12640	20825-20826	NN	denotes	A
T12641	20826-20828	,	denotes	,
T12642	20828-20829	LS	denotes	b
T12643	20830-20833	CC	denotes	and
T12644	20834-20835	LS	denotes	d
T12645	20835-20837	,	denotes	,
T12638	20837-20847	NNS	denotes	arrowheads
T12646	20847-20848	-RRB-	denotes	)
T12647	20848-20850	,	denotes	,
T12648	20850-20860	VBG	denotes	indicating
T12649	20861-20867	JJ	denotes	normal
T12651	20868-20872	NN	denotes	type
T12652	20873-20875	CD	denotes	II
T12653	20876-20880	NNS	denotes	AECs
T12650	20881-20896	NN	denotes	differentiation
T12654	20896-20897	.	denotes	.
T12655	20897-20926	sentence	denotes	Original magnification: 10X.
T12656	20898-20906	JJ	denotes	Original
T12657	20907-20920	NN	denotes	magnification
T12659	20920-20922	:	denotes	:
T12658	20922-20925	NN	denotes	10X
T12660	20925-20926	.	denotes	.
T12661	20926-21111	sentence	denotes	Panel B, left-hand panel, shows a representative result from Northern analyses of total RNA from wild-type, heterozygous and knockout P0 lungs for SP-A, SP-B, SP-C and SP-D expression.
T12662	20927-20932	NN	denotes	Panel
T12663	20933-20934	NN	denotes	B
T12665	20934-20936	,	denotes	,
T12666	20936-20940	JJ	denotes	left
T12668	20940-20941	HYPH	denotes	-
T12667	20941-20945	NN	denotes	hand
T12669	20946-20951	NN	denotes	panel
T12670	20951-20953	,	denotes	,
T12664	20953-20958	VBZ	denotes	shows
T12671	20959-20960	DT	denotes	a
T12673	20961-20975	JJ	denotes	representative
T12672	20976-20982	NN	denotes	result
T12674	20983-20987	IN	denotes	from
T12675	20988-20996	NNP	denotes	Northern
T12676	20997-21005	NNS	denotes	analyses
T12677	21006-21008	IN	denotes	of
T12678	21009-21014	JJ	denotes	total
T12679	21015-21018	NN	denotes	RNA
T12680	21019-21023	IN	denotes	from
T12681	21024-21028	JJ	denotes	wild
T12683	21028-21029	HYPH	denotes	-
T12682	21029-21033	NN	denotes	type
T12685	21033-21035	,	denotes	,
T12686	21035-21047	JJ	denotes	heterozygous
T12687	21048-21051	CC	denotes	and
T12688	21052-21060	NN	denotes	knockout
T12689	21061-21063	NN	denotes	P0
T12684	21064-21069	NNS	denotes	lungs
T12690	21070-21073	IN	denotes	for
T12691	21074-21076	NN	denotes	SP
T12693	21076-21077	HYPH	denotes	-
T12692	21077-21078	NN	denotes	A
T12695	21078-21080	,	denotes	,
T12696	21080-21082	NN	denotes	SP
T12698	21082-21083	HYPH	denotes	-
T12697	21083-21084	NN	denotes	B
T12699	21084-21086	,	denotes	,
T12700	21086-21088	NN	denotes	SP
T12702	21088-21089	HYPH	denotes	-
T12701	21089-21090	NN	denotes	C
T12703	21091-21094	CC	denotes	and
T12704	21095-21097	NN	denotes	SP
T12706	21097-21098	HYPH	denotes	-
T12705	21098-21099	NN	denotes	D
T12694	21100-21110	NN	denotes	expression
T12707	21110-21111	.	denotes	.
T12708	21111-21283	sentence	denotes	Densitometry analyses showed the relative expression (n = 3, mean ± s.d.) of these surfactant protein mRNAs were not significantly different between these three genotypes.
T12709	21112-21124	NN	denotes	Densitometry
T12710	21125-21133	NNS	denotes	analyses
T12711	21134-21140	VBD	denotes	showed
T12712	21141-21144	DT	denotes	the
T12714	21145-21153	JJ	denotes	relative
T12713	21154-21164	NN	denotes	expression
T12716	21165-21166	-LRB-	denotes	(
T12718	21166-21167	NN	denotes	n
T12720	21168-21169	SYM	denotes	=
T12719	21170-21171	CD	denotes	3
T12721	21171-21173	,	denotes	,
T12722	21173-21177	NN	denotes	mean
T12723	21178-21179	SYM	denotes	±
T12717	21180-21184	NN	denotes	s.d.
T12724	21184-21185	-RRB-	denotes	)
T12725	21186-21188	IN	denotes	of
T12726	21189-21194	DT	denotes	these
T12728	21195-21205	NN	denotes	surfactant
T12729	21206-21213	NN	denotes	protein
T12727	21214-21219	NNS	denotes	mRNAs
T12715	21220-21224	VBD	denotes	were
T12730	21225-21228	RB	denotes	not
T12731	21229-21242	RB	denotes	significantly
T12732	21243-21252	JJ	denotes	different
T12733	21253-21260	IN	denotes	between
T12734	21261-21266	DT	denotes	these
T12736	21267-21272	CD	denotes	three
T12735	21273-21282	NNS	denotes	genotypes
T12737	21282-21283	.	denotes	.
T4548	21285-21290	NN	denotes	Dhcr7
T4550	21290-21291	SYM	denotes	-
T4551	21291-21292	SYM	denotes	/
T4552	21292-21293	SYM	denotes	-
T4549	21294-21299	NNS	denotes	lungs
T4553	21300-21306	VBD	denotes	showed
T4554	21307-21315	JJ	denotes	abnormal
T4555	21316-21331	NN	denotes	differentiation
T4556	21332-21334	IN	denotes	of
T4557	21335-21339	NN	denotes	type
T4558	21340-21341	CD	denotes	I
T4559	21342-21350	JJ	denotes	alveolar
T4561	21351-21361	JJ	denotes	epithelial
T4560	21362-21367	NNS	denotes	cells
T4562	21368-21369	-LRB-	denotes	(
T4563	21369-21373	NNS	denotes	AECs
T4564	21373-21374	-RRB-	denotes	)
T4565	21374-21542	sentence	denotes	At E20.5, the distal lung of wild type embryos consisted of saccular structures lined by flat type I AECs, and by cuboidal surfactant-producing type II AECs (Fig. 7A).
T4566	21375-21377	IN	denotes	At
T4568	21378-21383	NN	denotes	E20.5
T4569	21383-21385	,	denotes	,
T4570	21385-21388	DT	denotes	the
T4572	21389-21395	JJ	denotes	distal
T4571	21396-21400	NN	denotes	lung
T4573	21401-21403	IN	denotes	of
T4574	21404-21408	JJ	denotes	wild
T4575	21409-21413	NN	denotes	type
T4576	21414-21421	NNS	denotes	embryos
T4567	21422-21431	VBD	denotes	consisted
T4577	21432-21434	IN	denotes	of
T4578	21435-21443	JJ	denotes	saccular
T4579	21444-21454	NNS	denotes	structures
T4580	21455-21460	VBN	denotes	lined
T4581	21461-21463	IN	denotes	by
T4582	21464-21468	JJ	denotes	flat
T4584	21469-21473	NN	denotes	type
T4585	21474-21475	CD	denotes	I
T4583	21476-21480	NNS	denotes	AECs
T4586	21480-21482	,	denotes	,
T4587	21482-21485	CC	denotes	and
T4588	21486-21488	IN	denotes	by
T4589	21489-21497	JJ	denotes	cuboidal
T4591	21498-21508	NN	denotes	surfactant
T4593	21508-21509	HYPH	denotes	-
T4592	21509-21518	VBG	denotes	producing
T4594	21519-21523	NN	denotes	type
T4595	21524-21526	CD	denotes	II
T4590	21527-21531	NNS	denotes	AECs
T4596	21532-21533	-LRB-	denotes	(
T4597	21533-21537	NN	denotes	Fig.
T4598	21538-21540	CD	denotes	7A
T4599	21540-21541	-RRB-	denotes	)
T4600	21541-21542	.	denotes	.
T4601	21542-21634	sentence	denotes	In contrast, the formation of these typical saccules was impaired in Dhcr7 deficient lungs.
T4602	21543-21545	IN	denotes	In
T4604	21546-21554	NN	denotes	contrast
T4605	21554-21556	,	denotes	,
T4606	21556-21559	DT	denotes	the
T4607	21560-21569	NN	denotes	formation
T4608	21570-21572	IN	denotes	of
T4609	21573-21578	DT	denotes	these
T4611	21579-21586	JJ	denotes	typical
T4610	21587-21595	NNS	denotes	saccules
T4612	21596-21599	VBD	denotes	was
T4603	21600-21608	VBN	denotes	impaired
T4613	21609-21611	IN	denotes	in
T4614	21612-21617	NN	denotes	Dhcr7
T4615	21618-21627	JJ	denotes	deficient
T4616	21628-21633	NNS	denotes	lungs
T4617	21633-21634	.	denotes	.
T4618	21634-21829	sentence	denotes	Instead, these saccular structures were lined by a low columnar epithelium that lacked flattened type I-like cells with the presence of many undeveloped or arrested epithelial tubules (Fig. 7B).
T4619	21635-21642	RB	denotes	Instead
T4621	21642-21644	,	denotes	,
T4622	21644-21649	DT	denotes	these
T4624	21650-21658	JJ	denotes	saccular
T4623	21659-21669	NNS	denotes	structures
T4625	21670-21674	VBD	denotes	were
T4620	21675-21680	VBN	denotes	lined
T4626	21681-21683	IN	denotes	by
T4627	21684-21685	DT	denotes	a
T4629	21686-21689	JJ	denotes	low
T4630	21690-21698	JJ	denotes	columnar
T4628	21699-21709	NN	denotes	epithelium
T4631	21710-21714	WDT	denotes	that
T4632	21715-21721	VBD	denotes	lacked
T4633	21722-21731	JJ	denotes	flattened
T4635	21732-21736	NN	denotes	type
T4637	21737-21738	CD	denotes	I
T4638	21738-21739	HYPH	denotes	-
T4636	21739-21743	JJ	denotes	like
T4634	21744-21749	NNS	denotes	cells
T4639	21750-21754	IN	denotes	with
T4640	21755-21758	DT	denotes	the
T4641	21759-21767	NN	denotes	presence
T4642	21768-21770	IN	denotes	of
T4643	21771-21775	JJ	denotes	many
T4645	21776-21787	JJ	denotes	undeveloped
T4646	21788-21790	CC	denotes	or
T4647	21791-21799	JJ	denotes	arrested
T4648	21800-21810	JJ	denotes	epithelial
T4644	21811-21818	NNS	denotes	tubules
T4649	21819-21820	-LRB-	denotes	(
T4650	21820-21824	NN	denotes	Fig.
T4651	21825-21827	CD	denotes	7B
T4652	21827-21828	-RRB-	denotes	)
T4653	21828-21829	.	denotes	.
T4654	21829-22020	sentence	denotes	Consistent with disruption of type I AECs differentiation was the dramatic reduction of expression of T1α, an apical membrane protein marker of lung type I AECs (Fig. 7, cf. panels C and D).
T4655	21830-21840	JJ	denotes	Consistent
T4657	21841-21845	IN	denotes	with
T4658	21846-21856	NN	denotes	disruption
T4659	21857-21859	IN	denotes	of
T4660	21860-21864	NN	denotes	type
T4662	21865-21866	CD	denotes	I
T4661	21867-21871	NNS	denotes	AECs
T4663	21872-21887	NN	denotes	differentiation
T4656	21888-21891	VBD	denotes	was
T4664	21892-21895	DT	denotes	the
T4666	21896-21904	JJ	denotes	dramatic
T4665	21905-21914	NN	denotes	reduction
T4667	21915-21917	IN	denotes	of
T4668	21918-21928	NN	denotes	expression
T4669	21929-21931	IN	denotes	of
T4670	21932-21935	NN	denotes	T1α
T4671	21935-21937	,	denotes	,
T4672	21937-21939	DT	denotes	an
T4674	21940-21946	JJ	denotes	apical
T4675	21947-21955	NN	denotes	membrane
T4676	21956-21963	NN	denotes	protein
T4673	21964-21970	NN	denotes	marker
T4677	21971-21973	IN	denotes	of
T4678	21974-21978	NN	denotes	lung
T4680	21979-21983	NN	denotes	type
T4681	21984-21985	CD	denotes	I
T4679	21986-21990	NNS	denotes	AECs
T4682	21991-21992	-LRB-	denotes	(
T4684	21992-21996	NN	denotes	Fig.
T4685	21997-21998	CD	denotes	7
T4686	21998-22000	,	denotes	,
T4687	22000-22003	FW	denotes	cf.
T4688	22004-22010	NNS	denotes	panels
T4683	22011-22012	NN	denotes	C
T4689	22013-22016	CC	denotes	and
T4690	22017-22018	NN	denotes	D
T4691	22018-22019	-RRB-	denotes	)
T4692	22019-22020	.	denotes	.
T4693	22020-22188	sentence	denotes	Decreased immunostaining for AQP5, another type I AEC marker, was also noted in Dhcr7 deficient lungs at E20.5, compared to control lungs (Fig. 7, cf. panels E and F).
T4694	22021-22030	VBN	denotes	Decreased
T4695	22031-22045	NN	denotes	immunostaining
T4697	22046-22049	IN	denotes	for
T4698	22050-22054	NN	denotes	AQP5
T4699	22054-22056	,	denotes	,
T4700	22056-22063	DT	denotes	another
T4702	22064-22068	NN	denotes	type
T4703	22069-22070	CD	denotes	I
T4704	22071-22074	NN	denotes	AEC
T4701	22075-22081	NN	denotes	marker
T4705	22081-22083	,	denotes	,
T4706	22083-22086	VBD	denotes	was
T4707	22087-22091	RB	denotes	also
T4696	22092-22097	VBN	denotes	noted
T4708	22098-22100	IN	denotes	in
T4709	22101-22106	NN	denotes	Dhcr7
T4710	22107-22116	JJ	denotes	deficient
T4711	22117-22122	NNS	denotes	lungs
T4712	22123-22125	IN	denotes	at
T4713	22126-22131	NN	denotes	E20.5
T4714	22131-22133	,	denotes	,
T4715	22133-22141	VBN	denotes	compared
T4716	22142-22144	IN	denotes	to
T4717	22145-22152	NN	denotes	control
T4718	22153-22158	NNS	denotes	lungs
T4719	22159-22160	-LRB-	denotes	(
T4721	22160-22164	NN	denotes	Fig.
T4722	22165-22166	CD	denotes	7
T4723	22166-22168	,	denotes	,
T4724	22168-22171	FW	denotes	cf.
T4725	22172-22178	NNS	denotes	panels
T4720	22179-22180	NN	denotes	E
T4726	22181-22184	CC	denotes	and
T4727	22185-22186	NN	denotes	F
T4728	22186-22187	-RRB-	denotes	)
T4729	22187-22188	.	denotes	.
T4730	22188-22354	sentence	denotes	Megalin, a low density lipoprotein receptor (LDLR) gene family member, is expressed by embryonic epithelial cells and can function as an endocytic Shh receptor [32].
T4731	22189-22196	NN	denotes	Megalin
T4733	22196-22198	,	denotes	,
T4734	22198-22199	DT	denotes	a
T4736	22200-22203	JJ	denotes	low
T4737	22204-22211	NN	denotes	density
T4739	22212-22223	NN	denotes	lipoprotein
T4738	22224-22232	NN	denotes	receptor
T4740	22233-22234	-LRB-	denotes	(
T4741	22234-22238	NN	denotes	LDLR
T4742	22238-22239	-RRB-	denotes	)
T4743	22240-22244	NN	denotes	gene
T4744	22245-22251	NN	denotes	family
T4735	22252-22258	NN	denotes	member
T4745	22258-22260	,	denotes	,
T4746	22260-22262	VBZ	denotes	is
T4732	22263-22272	VBN	denotes	expressed
T4747	22273-22275	IN	denotes	by
T4748	22276-22285	JJ	denotes	embryonic
T4750	22286-22296	JJ	denotes	epithelial
T4749	22297-22302	NNS	denotes	cells
T4751	22303-22306	CC	denotes	and
T4752	22307-22310	MD	denotes	can
T4753	22311-22319	VB	denotes	function
T4754	22320-22322	IN	denotes	as
T4755	22323-22325	DT	denotes	an
T4757	22326-22335	JJ	denotes	endocytic
T4758	22336-22339	NN	denotes	Shh
T4756	22340-22348	NN	denotes	receptor
T4759	22349-22350	-LRB-	denotes	[
T4760	22350-22352	CD	denotes	32
T4761	22352-22353	-RRB-	denotes	]
T4762	22353-22354	.	denotes	.
T4763	22354-22503	sentence	denotes	Megalin was highly expressed on the distal epithelium at E20.5 from wild-type lungs, as well as type I AECs lining the developed saccules (Fig. 7G).
T4764	22355-22362	NN	denotes	Megalin
T4765	22363-22366	VBD	denotes	was
T4766	22367-22373	RB	denotes	highly
T4767	22374-22383	VBN	denotes	expressed
T4768	22384-22386	IN	denotes	on
T4769	22387-22390	DT	denotes	the
T4771	22391-22397	JJ	denotes	distal
T4770	22398-22408	NN	denotes	epithelium
T4772	22409-22411	IN	denotes	at
T4773	22412-22417	NN	denotes	E20.5
T4774	22418-22422	IN	denotes	from
T4775	22423-22427	JJ	denotes	wild
T4777	22427-22428	HYPH	denotes	-
T4776	22428-22432	NN	denotes	type
T4778	22433-22438	NNS	denotes	lungs
T4779	22438-22440	,	denotes	,
T4780	22440-22442	RB	denotes	as
T4782	22443-22447	RB	denotes	well
T4781	22448-22450	IN	denotes	as
T4783	22451-22455	NN	denotes	type
T4785	22456-22457	CD	denotes	I
T4784	22458-22462	NNS	denotes	AECs
T4786	22463-22469	VBG	denotes	lining
T4787	22470-22473	DT	denotes	the
T4789	22474-22483	VBN	denotes	developed
T4788	22484-22492	NNS	denotes	saccules
T4790	22493-22494	-LRB-	denotes	(
T4791	22494-22498	NN	denotes	Fig.
T4792	22499-22501	CD	denotes	7G
T4793	22501-22502	-RRB-	denotes	)
T4794	22502-22503	.	denotes	.
T4795	22503-22760	sentence	denotes	In contrast, though megalin staining was detected in developing saccules in knockout embryonic lungs, its expression was confined to undifferentiated cuboidal cells (Fig. 7H), further indicating a delay in type I AECs differentiation in the knockout lungs.
T4796	22504-22506	IN	denotes	In
T4798	22507-22515	NN	denotes	contrast
T4799	22515-22517	,	denotes	,
T4800	22517-22523	IN	denotes	though
T4802	22524-22531	NN	denotes	megalin
T4803	22532-22540	NN	denotes	staining
T4804	22541-22544	VBD	denotes	was
T4801	22545-22553	VBN	denotes	detected
T4805	22554-22556	IN	denotes	in
T4806	22557-22567	VBG	denotes	developing
T4807	22568-22576	NNS	denotes	saccules
T4808	22577-22579	IN	denotes	in
T4809	22580-22588	NN	denotes	knockout
T4811	22589-22598	JJ	denotes	embryonic
T4810	22599-22604	NNS	denotes	lungs
T4812	22604-22606	,	denotes	,
T4813	22606-22609	PRP$	denotes	its
T4814	22610-22620	NN	denotes	expression
T4815	22621-22624	VBD	denotes	was
T4797	22625-22633	VBN	denotes	confined
T4816	22634-22636	IN	denotes	to
T4817	22637-22653	JJ	denotes	undifferentiated
T4819	22654-22662	JJ	denotes	cuboidal
T4818	22663-22668	NNS	denotes	cells
T4820	22669-22670	-LRB-	denotes	(
T4821	22670-22674	NN	denotes	Fig.
T4822	22675-22677	CD	denotes	7H
T4823	22677-22678	-RRB-	denotes	)
T4824	22678-22680	,	denotes	,
T4825	22680-22687	RB	denotes	further
T4826	22688-22698	VBG	denotes	indicating
T4827	22699-22700	DT	denotes	a
T4828	22701-22706	NN	denotes	delay
T4829	22707-22709	IN	denotes	in
T4830	22710-22714	NN	denotes	type
T4832	22715-22716	CD	denotes	I
T4831	22717-22721	NNS	denotes	AECs
T4833	22722-22737	NN	denotes	differentiation
T4834	22738-22740	IN	denotes	in
T4835	22741-22744	DT	denotes	the
T4837	22745-22753	NN	denotes	knockout
T4836	22754-22759	NNS	denotes	lungs
T4838	22759-22760	.	denotes	.
T12874	22771-22787	NN	denotes	Characterization
T12875	22788-22790	IN	denotes	of
T12876	22791-22795	NN	denotes	type
T12878	22796-22797	CD	denotes	I
T12877	22798-22802	NNS	denotes	AECs
T12879	22803-22818	NN	denotes	differentiation
T12880	22818-22819	.	denotes	.
T12881	22819-23181	sentence	denotes	The normal distal saccules of wild-type (+/+) at near term (E20.5) showed typical type I (t1) and type II (t2) epithelial cells (panel A, arrowheads), but knockout (-/-) littermate lungs showed less developed saccules lined by columnar epithelial cells (panel B, black arrowheads), with partially arrested tubules in the distal lung (panel B, green arrowheads).
T12882	22820-22823	DT	denotes	The
T12884	22824-22830	JJ	denotes	normal
T12885	22831-22837	JJ	denotes	distal
T12883	22838-22846	NNS	denotes	saccules
T12887	22847-22849	IN	denotes	of
T12888	22850-22854	JJ	denotes	wild
T12890	22854-22855	HYPH	denotes	-
T12889	22855-22859	NN	denotes	type
T12891	22860-22861	-LRB-	denotes	(
T12892	22861-22862	SYM	denotes	+
T12894	22862-22863	SYM	denotes	/
T12893	22863-22864	SYM	denotes	+
T12895	22864-22865	-RRB-	denotes	)
T12896	22866-22868	IN	denotes	at
T12897	22869-22873	JJ	denotes	near
T12898	22874-22878	NN	denotes	term
T12899	22879-22880	-LRB-	denotes	(
T12900	22880-22885	NN	denotes	E20.5
T12901	22885-22886	-RRB-	denotes	)
T12886	22887-22893	VBD	denotes	showed
T12902	22894-22901	JJ	denotes	typical
T12903	22902-22906	NN	denotes	type
T12904	22907-22908	CD	denotes	I
T12905	22909-22910	-LRB-	denotes	(
T12906	22910-22912	NN	denotes	t1
T12907	22912-22913	-RRB-	denotes	)
T12908	22914-22917	CC	denotes	and
T12909	22918-22922	NN	denotes	type
T12911	22923-22925	CD	denotes	II
T12912	22926-22927	-LRB-	denotes	(
T12913	22927-22929	NN	denotes	t2
T12914	22929-22930	-RRB-	denotes	)
T12915	22931-22941	JJ	denotes	epithelial
T12910	22942-22947	NNS	denotes	cells
T12916	22948-22949	-LRB-	denotes	(
T12918	22949-22954	NN	denotes	panel
T12919	22955-22956	NN	denotes	A
T12920	22956-22958	,	denotes	,
T12917	22958-22968	NNS	denotes	arrowheads
T12921	22968-22969	-RRB-	denotes	)
T12922	22969-22971	,	denotes	,
T12923	22971-22974	CC	denotes	but
T12924	22975-22983	NN	denotes	knockout
T12926	22984-22985	-LRB-	denotes	(
T12927	22985-22986	SYM	denotes	-
T12929	22986-22987	SYM	denotes	/
T12928	22987-22988	SYM	denotes	-
T12930	22988-22989	-RRB-	denotes	)
T12931	22990-23000	NN	denotes	littermate
T12925	23001-23006	NNS	denotes	lungs
T12932	23007-23013	VBD	denotes	showed
T12933	23014-23018	RBR	denotes	less
T12934	23019-23028	JJ	denotes	developed
T12935	23029-23037	NNS	denotes	saccules
T12936	23038-23043	VBN	denotes	lined
T12937	23044-23046	IN	denotes	by
T12938	23047-23055	JJ	denotes	columnar
T12940	23056-23066	JJ	denotes	epithelial
T12939	23067-23072	NNS	denotes	cells
T12941	23073-23074	-LRB-	denotes	(
T12943	23074-23079	NN	denotes	panel
T12944	23080-23081	NN	denotes	B
T12945	23081-23083	,	denotes	,
T12946	23083-23088	JJ	denotes	black
T12942	23089-23099	NNS	denotes	arrowheads
T12947	23099-23100	-RRB-	denotes	)
T12948	23100-23102	,	denotes	,
T12949	23102-23106	IN	denotes	with
T12950	23107-23116	RB	denotes	partially
T12951	23117-23125	VBN	denotes	arrested
T12952	23126-23133	NNS	denotes	tubules
T12953	23134-23136	IN	denotes	in
T12954	23137-23140	DT	denotes	the
T12956	23141-23147	JJ	denotes	distal
T12955	23148-23152	NN	denotes	lung
T12957	23153-23154	-LRB-	denotes	(
T12959	23154-23159	NN	denotes	panel
T12960	23160-23161	NN	denotes	B
T12961	23161-23163	,	denotes	,
T12962	23163-23168	JJ	denotes	green
T12958	23169-23179	NNS	denotes	arrowheads
T12963	23179-23180	-RRB-	denotes	)
T12964	23180-23181	.	denotes	.
T12965	23181-23349	sentence	denotes	Immunostaining for type I cell markers T1α and AQP5 demonstrated an intense staining in flat epithelial cells in wild-type lungs at E20.5 (panels C and E, arrowheads).
T12966	23182-23196	NN	denotes	Immunostaining
T12968	23197-23200	IN	denotes	for
T12969	23201-23205	NN	denotes	type
T12971	23206-23207	CD	denotes	I
T12972	23208-23212	NN	denotes	cell
T12970	23213-23220	NNS	denotes	markers
T12973	23221-23224	NN	denotes	T1α
T12974	23225-23228	CC	denotes	and
T12975	23229-23233	NN	denotes	AQP5
T12967	23234-23246	VBD	denotes	demonstrated
T12976	23247-23249	DT	denotes	an
T12978	23250-23257	JJ	denotes	intense
T12977	23258-23266	NN	denotes	staining
T12979	23267-23269	IN	denotes	in
T12980	23270-23274	JJ	denotes	flat
T12982	23275-23285	JJ	denotes	epithelial
T12981	23286-23291	NNS	denotes	cells
T12983	23292-23294	IN	denotes	in
T12984	23295-23299	JJ	denotes	wild
T12986	23299-23300	HYPH	denotes	-
T12985	23300-23304	NN	denotes	type
T12987	23305-23310	NNS	denotes	lungs
T12988	23311-23313	IN	denotes	at
T12989	23314-23319	NN	denotes	E20.5
T12990	23320-23321	-LRB-	denotes	(
T12992	23321-23327	NNS	denotes	panels
T12993	23328-23329	NN	denotes	C
T12994	23330-23333	CC	denotes	and
T12995	23334-23335	NN	denotes	E
T12996	23335-23337	,	denotes	,
T12991	23337-23347	NNS	denotes	arrowheads
T12997	23347-23348	-RRB-	denotes	)
T12998	23348-23349	.	denotes	.
T12999	23349-23471	sentence	denotes	In contrast, in knockout littermate lungs, T1α and AQP5 staining was dramatically decreased (panels D and F, arrowheads).
T13000	23350-23352	IN	denotes	In
T13002	23353-23361	NN	denotes	contrast
T13003	23361-23363	,	denotes	,
T13004	23363-23365	IN	denotes	in
T13005	23366-23374	NN	denotes	knockout
T13007	23375-23385	NN	denotes	littermate
T13006	23386-23391	NNS	denotes	lungs
T13008	23391-23393	,	denotes	,
T13009	23393-23396	NN	denotes	T1α
T13011	23397-23400	CC	denotes	and
T13012	23401-23405	NN	denotes	AQP5
T13010	23406-23414	NN	denotes	staining
T13013	23415-23418	VBD	denotes	was
T13014	23419-23431	RB	denotes	dramatically
T13001	23432-23441	VBN	denotes	decreased
T13015	23442-23443	-LRB-	denotes	(
T13017	23443-23449	NNS	denotes	panels
T13018	23450-23451	NN	denotes	D
T13019	23452-23455	CC	denotes	and
T13020	23456-23457	NN	denotes	F
T13021	23457-23459	,	denotes	,
T13016	23459-23469	NNS	denotes	arrowheads
T13022	23469-23470	-RRB-	denotes	)
T13023	23470-23471	.	denotes	.
T13024	23471-23662	sentence	denotes	Megalin expression (Meg) was detected in the airway epithelial cells, but not in proximal bronchiole epithelial cells (data not shown) in the lungs from both wild-type and knockout at E20.5.
T13025	23472-23479	NN	denotes	Megalin
T13026	23480-23490	NN	denotes	expression
T13028	23491-23492	-LRB-	denotes	(
T13029	23492-23495	NN	denotes	Meg
T13030	23495-23496	-RRB-	denotes	)
T13031	23497-23500	VBD	denotes	was
T13027	23501-23509	VBN	denotes	detected
T13032	23510-23512	IN	denotes	in
T13033	23513-23516	DT	denotes	the
T13035	23517-23523	NN	denotes	airway
T13036	23524-23534	JJ	denotes	epithelial
T13034	23535-23540	NNS	denotes	cells
T13037	23540-23542	,	denotes	,
T13038	23542-23545	CC	denotes	but
T13039	23546-23549	RB	denotes	not
T13040	23550-23552	IN	denotes	in
T13041	23553-23561	JJ	denotes	proximal
T13043	23562-23572	NN	denotes	bronchiole
T13044	23573-23583	JJ	denotes	epithelial
T13042	23584-23589	NNS	denotes	cells
T13045	23590-23591	-LRB-	denotes	(
T13047	23591-23595	NNS	denotes	data
T13048	23596-23599	RB	denotes	not
T13046	23600-23605	VBN	denotes	shown
T13049	23605-23606	-RRB-	denotes	)
T13050	23607-23609	IN	denotes	in
T13051	23610-23613	DT	denotes	the
T13052	23614-23619	NNS	denotes	lungs
T13053	23620-23624	IN	denotes	from
T13054	23625-23629	CC	denotes	both
T13056	23630-23634	JJ	denotes	wild
T13057	23634-23635	HYPH	denotes	-
T13055	23635-23639	NN	denotes	type
T13058	23640-23643	CC	denotes	and
T13059	23644-23652	NN	denotes	knockout
T13060	23653-23655	IN	denotes	at
T13061	23656-23661	NN	denotes	E20.5
T13062	23661-23662	.	denotes	.
T13063	23662-23809	sentence	denotes	The elongated megalin-positive epithelial cells, lining the normally developed sacculi, were readily evident in many areas of the wild-type lungs.
T13064	23663-23666	DT	denotes	The
T13066	23667-23676	VBN	denotes	elongated
T13067	23677-23684	NN	denotes	megalin
T13069	23684-23685	HYPH	denotes	-
T13068	23685-23693	JJ	denotes	positive
T13070	23694-23704	JJ	denotes	epithelial
T13065	23705-23710	NNS	denotes	cells
T13072	23710-23712	,	denotes	,
T13073	23712-23718	VBG	denotes	lining
T13074	23719-23722	DT	denotes	the
T13076	23723-23731	RB	denotes	normally
T13077	23732-23741	VBN	denotes	developed
T13075	23742-23749	NNS	denotes	sacculi
T13078	23749-23751	,	denotes	,
T13071	23751-23755	VBD	denotes	were
T13079	23756-23763	RB	denotes	readily
T13080	23764-23771	JJ	denotes	evident
T13081	23772-23774	IN	denotes	in
T13082	23775-23779	JJ	denotes	many
T13083	23780-23785	NNS	denotes	areas
T13084	23786-23788	IN	denotes	of
T13085	23789-23792	DT	denotes	the
T13087	23793-23797	JJ	denotes	wild
T13089	23797-23798	HYPH	denotes	-
T13088	23798-23802	NN	denotes	type
T13086	23803-23808	NNS	denotes	lungs
T13090	23808-23809	.	denotes	.
T13091	23809-24005	sentence	denotes	In contrast, the terminal epithelial cells positively stained by megalin antibodies in knockout lungs were comprised mainly of undifferentiated cuboidal cells (compare the arrowheads in G and H).
T13092	23810-23812	IN	denotes	In
T13094	23813-23821	NN	denotes	contrast
T13095	23821-23823	,	denotes	,
T13096	23823-23826	DT	denotes	the
T13098	23827-23835	JJ	denotes	terminal
T13099	23836-23846	JJ	denotes	epithelial
T13097	23847-23852	NNS	denotes	cells
T13100	23853-23863	RB	denotes	positively
T13101	23864-23871	VBN	denotes	stained
T13102	23872-23874	IN	denotes	by
T13103	23875-23882	NN	denotes	megalin
T13104	23883-23893	NNS	denotes	antibodies
T13105	23894-23896	IN	denotes	in
T13106	23897-23905	NN	denotes	knockout
T13107	23906-23911	NNS	denotes	lungs
T13108	23912-23916	VBD	denotes	were
T13093	23917-23926	VBN	denotes	comprised
T13109	23927-23933	RB	denotes	mainly
T13110	23934-23936	IN	denotes	of
T13111	23937-23953	JJ	denotes	undifferentiated
T13113	23954-23962	JJ	denotes	cuboidal
T13112	23963-23968	NNS	denotes	cells
T13114	23969-23970	-LRB-	denotes	(
T13115	23970-23977	VB	denotes	compare
T13116	23978-23981	DT	denotes	the
T13117	23982-23992	NNS	denotes	arrowheads
T13118	23993-23995	IN	denotes	in
T13119	23996-23997	NN	denotes	G
T13120	23998-24001	CC	denotes	and
T13121	24002-24003	NN	denotes	H
T13122	24003-24004	-RRB-	denotes	)
T13123	24004-24005	.	denotes	.
T13124	24005-24065	sentence	denotes	Original magnification: A, B, G and H are 60X, C-F are 40X.
T13125	24006-24014	JJ	denotes	Original
T13126	24015-24028	NN	denotes	magnification
T13128	24028-24030	:	denotes	:
T13129	24030-24031	NN	denotes	A
T13131	24031-24033	,	denotes	,
T13132	24033-24034	NN	denotes	B
T13133	24034-24036	,	denotes	,
T13134	24036-24037	NN	denotes	G
T13135	24038-24041	CC	denotes	and
T13136	24042-24043	NN	denotes	H
T13130	24044-24047	VBP	denotes	are
T13137	24048-24051	NN	denotes	60X
T13138	24051-24053	,	denotes	,
T13139	24053-24054	NN	denotes	C
T13141	24054-24055	HYPH	denotes	-
T13140	24055-24056	NN	denotes	F
T13127	24057-24060	VBP	denotes	are
T13142	24061-24064	NN	denotes	40X
T13143	24064-24065	.	denotes	.
T5051	24067-24074	VBN	denotes	Delayed
T5053	24075-24083	JJ	denotes	vascular
T5052	24084-24095	NN	denotes	development
T5054	24096-24098	IN	denotes	in
T5055	24099-24104	NN	denotes	Dhcr7
T5057	24104-24105	SYM	denotes	-
T5058	24105-24106	SYM	denotes	/
T5059	24106-24107	SYM	denotes	-
T5056	24108-24113	NNS	denotes	lungs
T5060	24113-24209	sentence	denotes	There is a close relationship between blood vessel development and lung structural development.
T5061	24114-24119	EX	denotes	There
T5062	24120-24122	VBZ	denotes	is
T5063	24123-24124	DT	denotes	a
T5065	24125-24130	JJ	denotes	close
T5064	24131-24143	NN	denotes	relationship
T5066	24144-24151	IN	denotes	between
T5067	24152-24157	NN	denotes	blood
T5068	24158-24164	NN	denotes	vessel
T5069	24165-24176	NN	denotes	development
T5070	24177-24180	CC	denotes	and
T5071	24181-24185	NN	denotes	lung
T5073	24186-24196	JJ	denotes	structural
T5072	24197-24208	NN	denotes	development
T5074	24208-24209	.	denotes	.
T5075	24209-24416	sentence	denotes	At later lung developmental stages, timed capillary bed development is essential for the formation of mature alveolar gas-exchange, with a loss of mesenchyme separating the capillary beds and the AECs [19].
T5076	24210-24212	IN	denotes	At
T5078	24213-24218	JJR	denotes	later
T5080	24219-24223	NN	denotes	lung
T5081	24224-24237	JJ	denotes	developmental
T5079	24238-24244	NNS	denotes	stages
T5082	24244-24246	,	denotes	,
T5083	24246-24251	VBN	denotes	timed
T5085	24252-24261	JJ	denotes	capillary
T5086	24262-24265	NN	denotes	bed
T5084	24266-24277	NN	denotes	development
T5077	24278-24280	VBZ	denotes	is
T5087	24281-24290	JJ	denotes	essential
T5088	24291-24294	IN	denotes	for
T5089	24295-24298	DT	denotes	the
T5090	24299-24308	NN	denotes	formation
T5091	24309-24311	IN	denotes	of
T5092	24312-24318	JJ	denotes	mature
T5094	24319-24327	JJ	denotes	alveolar
T5095	24328-24331	NN	denotes	gas
T5096	24331-24332	HYPH	denotes	-
T5093	24332-24340	NN	denotes	exchange
T5097	24340-24342	,	denotes	,
T5098	24342-24346	IN	denotes	with
T5099	24347-24348	DT	denotes	a
T5100	24349-24353	NN	denotes	loss
T5102	24354-24356	IN	denotes	of
T5103	24357-24367	NN	denotes	mesenchyme
T5101	24368-24378	VBG	denotes	separating
T5104	24379-24382	DT	denotes	the
T5106	24383-24392	JJ	denotes	capillary
T5105	24393-24397	NNS	denotes	beds
T5107	24398-24401	CC	denotes	and
T5108	24402-24405	DT	denotes	the
T5109	24406-24410	NNS	denotes	AECs
T5110	24411-24412	-LRB-	denotes	[
T5111	24412-24414	CD	denotes	19
T5112	24414-24415	-RRB-	denotes	]
T5113	24415-24416	.	denotes	.
T5114	24416-24554	sentence	denotes	In Dhcr7-/- lungs at late gestational stages, the relative abundance of mesenchyme was consistent with the general arrest in sacculation.
T5115	24417-24419	IN	denotes	In
T5117	24420-24425	NN	denotes	Dhcr7
T5119	24425-24426	SYM	denotes	-
T5120	24426-24427	SYM	denotes	/
T5121	24427-24428	SYM	denotes	-
T5118	24429-24434	NNS	denotes	lungs
T5122	24435-24437	IN	denotes	at
T5123	24438-24442	JJ	denotes	late
T5125	24443-24454	JJ	denotes	gestational
T5124	24455-24461	NNS	denotes	stages
T5126	24461-24463	,	denotes	,
T5127	24463-24466	DT	denotes	the
T5129	24467-24475	JJ	denotes	relative
T5128	24476-24485	NN	denotes	abundance
T5130	24486-24488	IN	denotes	of
T5131	24489-24499	NN	denotes	mesenchyme
T5116	24500-24503	VBD	denotes	was
T5132	24504-24514	JJ	denotes	consistent
T5133	24515-24519	IN	denotes	with
T5134	24520-24523	DT	denotes	the
T5136	24524-24531	JJ	denotes	general
T5135	24532-24538	NN	denotes	arrest
T5137	24539-24541	IN	denotes	in
T5138	24542-24553	NN	denotes	sacculation
T5139	24553-24554	.	denotes	.
T5140	24554-24832	sentence	denotes	To investigate whether the delayed lung sacculation also involved abnormal blood vessel development, immunostaining for the α-isoform of caveolin-1 (cav-1α) and platelet endothelial cell adhesion molecular (PECAM-1) were performed on sections from wild-type and Dhcr7-/- lungs.
T5141	24555-24557	TO	denotes	To
T5142	24558-24569	VB	denotes	investigate
T5144	24570-24577	IN	denotes	whether
T5146	24578-24581	DT	denotes	the
T5148	24582-24589	VBN	denotes	delayed
T5149	24590-24594	NN	denotes	lung
T5147	24595-24606	NN	denotes	sacculation
T5150	24607-24611	RB	denotes	also
T5145	24612-24620	VBD	denotes	involved
T5151	24621-24629	JJ	denotes	abnormal
T5153	24630-24635	NN	denotes	blood
T5154	24636-24642	NN	denotes	vessel
T5152	24643-24654	NN	denotes	development
T5155	24654-24656	,	denotes	,
T5156	24656-24670	VBG	denotes	immunostaining
T5157	24671-24674	IN	denotes	for
T5158	24675-24678	DT	denotes	the
T5160	24679-24680	NN	denotes	α
T5161	24680-24681	HYPH	denotes	-
T5159	24681-24688	NN	denotes	isoform
T5162	24689-24691	IN	denotes	of
T5163	24692-24700	NN	denotes	caveolin
T5164	24700-24701	HYPH	denotes	-
T5165	24701-24702	CD	denotes	1
T5166	24703-24704	-LRB-	denotes	(
T5167	24704-24707	NN	denotes	cav
T5169	24707-24708	HYPH	denotes	-
T5168	24708-24710	NN	denotes	1α
T5170	24710-24711	-RRB-	denotes	)
T5171	24712-24715	CC	denotes	and
T5172	24716-24724	NN	denotes	platelet
T5174	24725-24736	JJ	denotes	endothelial
T5175	24737-24741	NN	denotes	cell
T5173	24742-24750	NN	denotes	adhesion
T5176	24751-24760	JJ	denotes	molecular
T5177	24761-24762	-LRB-	denotes	(
T5178	24762-24767	NN	denotes	PECAM
T5179	24767-24768	HYPH	denotes	-
T5180	24768-24769	CD	denotes	1
T5181	24769-24770	-RRB-	denotes	)
T5182	24771-24775	VBD	denotes	were
T5143	24776-24785	VBN	denotes	performed
T5183	24786-24788	IN	denotes	on
T5184	24789-24797	NNS	denotes	sections
T5185	24798-24802	IN	denotes	from
T5186	24803-24807	JJ	denotes	wild
T5188	24807-24808	HYPH	denotes	-
T5187	24808-24812	NN	denotes	type
T5190	24813-24816	CC	denotes	and
T5191	24817-24822	NN	denotes	Dhcr7
T5192	24822-24823	SYM	denotes	-
T5193	24823-24824	SYM	denotes	/
T5194	24824-24825	SYM	denotes	-
T5189	24826-24831	NNS	denotes	lungs
T5195	24831-24832	.	denotes	.
T5196	24832-24947	sentence	denotes	In the developing lung, cav-1α is expressed strictly in the endothelium of developing capillary and blood vessels.
T5197	24833-24835	IN	denotes	In
T5199	24836-24839	DT	denotes	the
T5201	24840-24850	VBG	denotes	developing
T5200	24851-24855	NN	denotes	lung
T5202	24855-24857	,	denotes	,
T5203	24857-24860	NN	denotes	cav
T5205	24860-24861	HYPH	denotes	-
T5204	24861-24863	NN	denotes	1α
T5206	24864-24866	VBZ	denotes	is
T5198	24867-24876	VBN	denotes	expressed
T5207	24877-24885	RB	denotes	strictly
T5208	24886-24888	IN	denotes	in
T5209	24889-24892	DT	denotes	the
T5210	24893-24904	NN	denotes	endothelium
T5211	24905-24907	IN	denotes	of
T5212	24908-24918	VBG	denotes	developing
T5213	24919-24928	NN	denotes	capillary
T5214	24929-24932	CC	denotes	and
T5215	24933-24938	NN	denotes	blood
T5216	24939-24946	NNS	denotes	vessels
T5217	24946-24947	.	denotes	.
T5218	24947-25074	sentence	denotes	Cav-1α is present in the terminally differentiated type I AECs, but is not detectable in its progenitors or type II AECs [33].
T5219	24948-24951	NN	denotes	Cav
T5221	24951-24952	HYPH	denotes	-
T5220	24952-24954	NN	denotes	1α
T5222	24955-24957	VBZ	denotes	is
T5223	24958-24965	JJ	denotes	present
T5224	24966-24968	IN	denotes	in
T5225	24969-24972	DT	denotes	the
T5227	24973-24983	RB	denotes	terminally
T5228	24984-24998	JJ	denotes	differentiated
T5229	24999-25003	NN	denotes	type
T5230	25004-25005	CD	denotes	I
T5226	25006-25010	NNS	denotes	AECs
T5231	25010-25012	,	denotes	,
T5232	25012-25015	CC	denotes	but
T5233	25016-25018	VBZ	denotes	is
T5234	25019-25022	RB	denotes	not
T5235	25023-25033	JJ	denotes	detectable
T5236	25034-25036	IN	denotes	in
T5237	25037-25040	PRP$	denotes	its
T5238	25041-25052	NNS	denotes	progenitors
T5239	25053-25055	CC	denotes	or
T5240	25056-25060	NN	denotes	type
T5242	25061-25063	CD	denotes	II
T5241	25064-25068	NNS	denotes	AECs
T5243	25069-25070	-LRB-	denotes	[
T5244	25070-25072	CD	denotes	33
T5245	25072-25073	-RRB-	denotes	]
T5246	25073-25074	.	denotes	.
T5247	25074-25319	sentence	denotes	At E14.5, both the epithelium and mesenchyme were negative for cav-1α staining, and cav-1α was detected in the developing blood vessels and capillaries, with no differences observed between wild-type and Dhcr7-/- lungs (Fig. 8, panels A and B).
T5248	25075-25077	IN	denotes	At
T5250	25078-25083	NN	denotes	E14.5
T5251	25083-25085	,	denotes	,
T5252	25085-25089	CC	denotes	both
T5254	25090-25093	DT	denotes	the
T5253	25094-25104	NN	denotes	epithelium
T5255	25105-25108	CC	denotes	and
T5256	25109-25119	NN	denotes	mesenchyme
T5249	25120-25124	VBD	denotes	were
T5257	25125-25133	JJ	denotes	negative
T5258	25134-25137	IN	denotes	for
T5259	25138-25141	NN	denotes	cav
T5261	25141-25142	HYPH	denotes	-
T5260	25142-25144	NN	denotes	1α
T5262	25145-25153	NN	denotes	staining
T5263	25153-25155	,	denotes	,
T5264	25155-25158	CC	denotes	and
T5265	25159-25162	NN	denotes	cav
T5267	25162-25163	HYPH	denotes	-
T5266	25163-25165	NN	denotes	1α
T5269	25166-25169	VBD	denotes	was
T5268	25170-25178	VBN	denotes	detected
T5270	25179-25181	IN	denotes	in
T5271	25182-25185	DT	denotes	the
T5273	25186-25196	VBG	denotes	developing
T5274	25197-25202	NN	denotes	blood
T5272	25203-25210	NNS	denotes	vessels
T5275	25211-25214	CC	denotes	and
T5276	25215-25226	NNS	denotes	capillaries
T5277	25226-25228	,	denotes	,
T5278	25228-25232	IN	denotes	with
T5280	25233-25235	DT	denotes	no
T5281	25236-25247	NNS	denotes	differences
T5279	25248-25256	VBN	denotes	observed
T5282	25257-25264	IN	denotes	between
T5283	25265-25269	JJ	denotes	wild
T5285	25269-25270	HYPH	denotes	-
T5284	25270-25274	NN	denotes	type
T5287	25275-25278	CC	denotes	and
T5288	25279-25284	NN	denotes	Dhcr7
T5289	25284-25285	SYM	denotes	-
T5290	25285-25286	SYM	denotes	/
T5291	25286-25287	SYM	denotes	-
T5286	25288-25293	NNS	denotes	lungs
T5292	25294-25295	-LRB-	denotes	(
T5294	25295-25299	NN	denotes	Fig.
T5295	25300-25301	CD	denotes	8
T5296	25301-25303	,	denotes	,
T5297	25303-25309	NNS	denotes	panels
T5293	25310-25311	NN	denotes	A
T5298	25312-25315	CC	denotes	and
T5299	25316-25317	NN	denotes	B
T5300	25317-25318	-RRB-	denotes	)
T5301	25318-25319	.	denotes	.
T5302	25319-25460	sentence	denotes	Cav-1α immunostaining demonstrated that the vascular development proceeded rapidly during saccular stages in normal lungs (E17.5 and E20.5).
T5303	25320-25323	NN	denotes	Cav
T5305	25323-25324	HYPH	denotes	-
T5304	25324-25326	NN	denotes	1α
T5306	25327-25341	NN	denotes	immunostaining
T5307	25342-25354	VBD	denotes	demonstrated
T5308	25355-25359	IN	denotes	that
T5310	25360-25363	DT	denotes	the
T5312	25364-25372	JJ	denotes	vascular
T5311	25373-25384	NN	denotes	development
T5309	25385-25394	VBD	denotes	proceeded
T5313	25395-25402	RB	denotes	rapidly
T5314	25403-25409	IN	denotes	during
T5315	25410-25418	JJ	denotes	saccular
T5316	25419-25425	NNS	denotes	stages
T5317	25426-25428	IN	denotes	in
T5318	25429-25435	JJ	denotes	normal
T5319	25436-25441	NNS	denotes	lungs
T5320	25442-25443	-LRB-	denotes	(
T5321	25443-25448	NN	denotes	E17.5
T5322	25449-25452	CC	denotes	and
T5323	25453-25458	NN	denotes	E20.5
T5324	25458-25459	-RRB-	denotes	)
T5325	25459-25460	.	denotes	.
T5326	25460-25682	sentence	denotes	In near-term normal lungs (E20.5), an extensive vascular network, associated with markedly thinned septation walls of well-developed alveoli and close apposition to flat type I AECs, was observed (Fig. 8, panels C and E).
T5327	25461-25463	IN	denotes	In
T5329	25464-25468	JJ	denotes	near
T5331	25468-25469	HYPH	denotes	-
T5330	25469-25473	NN	denotes	term
T5333	25474-25480	JJ	denotes	normal
T5332	25481-25486	NNS	denotes	lungs
T5334	25487-25488	-LRB-	denotes	(
T5335	25488-25493	NN	denotes	E20.5
T5336	25493-25494	-RRB-	denotes	)
T5337	25494-25496	,	denotes	,
T5338	25496-25498	DT	denotes	an
T5340	25499-25508	JJ	denotes	extensive
T5341	25509-25517	JJ	denotes	vascular
T5339	25518-25525	NN	denotes	network
T5342	25525-25527	,	denotes	,
T5343	25527-25537	VBN	denotes	associated
T5344	25538-25542	IN	denotes	with
T5345	25543-25551	RB	denotes	markedly
T5346	25552-25559	VBN	denotes	thinned
T5348	25560-25569	NN	denotes	septation
T5347	25570-25575	NNS	denotes	walls
T5349	25576-25578	IN	denotes	of
T5350	25579-25583	RB	denotes	well
T5352	25583-25584	HYPH	denotes	-
T5351	25584-25593	VBN	denotes	developed
T5353	25594-25601	NNS	denotes	alveoli
T5354	25602-25605	CC	denotes	and
T5355	25606-25611	JJ	denotes	close
T5356	25612-25622	NN	denotes	apposition
T5357	25623-25625	IN	denotes	to
T5358	25626-25630	JJ	denotes	flat
T5360	25631-25635	NN	denotes	type
T5361	25636-25637	CD	denotes	I
T5359	25638-25642	NNS	denotes	AECs
T5362	25642-25644	,	denotes	,
T5363	25644-25647	VBD	denotes	was
T5328	25648-25656	VBN	denotes	observed
T5364	25657-25658	-LRB-	denotes	(
T5366	25658-25662	NN	denotes	Fig.
T5367	25663-25664	CD	denotes	8
T5368	25664-25666	,	denotes	,
T5369	25666-25672	NNS	denotes	panels
T5365	25673-25674	NN	denotes	C
T5370	25675-25678	CC	denotes	and
T5371	25679-25680	NN	denotes	E
T5372	25680-25681	-RRB-	denotes	)
T5373	25681-25682	.	denotes	.
T5374	25682-25830	sentence	denotes	In contrast, the cav-1α patterns in lungs from Dhcr7-/- embryos revealed a relatively undeveloped pulmonary capillary bed (Fig. 8, panels D and F).
T5375	25683-25685	IN	denotes	In
T5377	25686-25694	NN	denotes	contrast
T5378	25694-25696	,	denotes	,
T5379	25696-25699	DT	denotes	the
T5381	25700-25703	NN	denotes	cav
T5383	25703-25704	HYPH	denotes	-
T5382	25704-25706	NN	denotes	1α
T5380	25707-25715	NNS	denotes	patterns
T5384	25716-25718	IN	denotes	in
T5385	25719-25724	NNS	denotes	lungs
T5386	25725-25729	IN	denotes	from
T5387	25730-25735	NN	denotes	Dhcr7
T5389	25735-25736	SYM	denotes	-
T5390	25736-25737	SYM	denotes	/
T5391	25737-25738	SYM	denotes	-
T5388	25739-25746	NNS	denotes	embryos
T5376	25747-25755	VBD	denotes	revealed
T5392	25756-25757	DT	denotes	a
T5394	25758-25768	RB	denotes	relatively
T5395	25769-25780	JJ	denotes	undeveloped
T5396	25781-25790	JJ	denotes	pulmonary
T5397	25791-25800	JJ	denotes	capillary
T5393	25801-25804	NN	denotes	bed
T5398	25805-25806	-LRB-	denotes	(
T5400	25806-25810	NN	denotes	Fig.
T5401	25811-25812	CD	denotes	8
T5402	25812-25814	,	denotes	,
T5403	25814-25820	NNS	denotes	panels
T5399	25821-25822	NN	denotes	D
T5404	25823-25826	CC	denotes	and
T5405	25827-25828	NN	denotes	F
T5406	25828-25829	-RRB-	denotes	)
T5407	25829-25830	.	denotes	.
T5408	25830-26061	sentence	denotes	At E20.5, cav-1α stained cells in knockout lungs were embedded in a relatively thick mesenchyme surrounding undeveloped epithelial tubules, and these epithelial cells remained cuboidal, instead of flattening out (Fig. 8, panel F).
T5409	25831-25833	IN	denotes	At
T5411	25834-25839	NN	denotes	E20.5
T5412	25839-25841	,	denotes	,
T5413	25841-25844	NN	denotes	cav
T5415	25844-25845	HYPH	denotes	-
T5414	25845-25847	NN	denotes	1α
T5416	25848-25855	VBN	denotes	stained
T5417	25856-25861	NNS	denotes	cells
T5418	25862-25864	IN	denotes	in
T5419	25865-25873	NN	denotes	knockout
T5420	25874-25879	NNS	denotes	lungs
T5421	25880-25884	VBD	denotes	were
T5410	25885-25893	VBN	denotes	embedded
T5422	25894-25896	IN	denotes	in
T5423	25897-25898	DT	denotes	a
T5425	25899-25909	RB	denotes	relatively
T5426	25910-25915	JJ	denotes	thick
T5424	25916-25926	NN	denotes	mesenchyme
T5427	25927-25938	VBG	denotes	surrounding
T5428	25939-25950	JJ	denotes	undeveloped
T5430	25951-25961	JJ	denotes	epithelial
T5429	25962-25969	NNS	denotes	tubules
T5431	25969-25971	,	denotes	,
T5432	25971-25974	CC	denotes	and
T5433	25975-25980	DT	denotes	these
T5435	25981-25991	JJ	denotes	epithelial
T5434	25992-25997	NNS	denotes	cells
T5436	25998-26006	VBD	denotes	remained
T5437	26007-26015	JJ	denotes	cuboidal
T5438	26015-26017	,	denotes	,
T5439	26017-26024	RB	denotes	instead
T5440	26025-26027	IN	denotes	of
T5441	26028-26038	VBG	denotes	flattening
T5442	26039-26042	RP	denotes	out
T5443	26043-26044	-LRB-	denotes	(
T5445	26044-26048	NN	denotes	Fig.
T5446	26049-26050	CD	denotes	8
T5447	26050-26052	,	denotes	,
T5448	26052-26057	NN	denotes	panel
T5444	26058-26059	NN	denotes	F
T5449	26059-26060	-RRB-	denotes	)
T5450	26060-26061	.	denotes	.
T5451	26061-26225	sentence	denotes	PECAM-1 staining at E20.5 confirmed the delayed lung vascular development at these late gestational stages in Dhcr7 deficient embryos (Fig. 8, cf. panels G and H).
T5452	26062-26067	NN	denotes	PECAM
T5454	26067-26068	HYPH	denotes	-
T5455	26068-26069	CD	denotes	1
T5453	26070-26078	NN	denotes	staining
T5457	26079-26081	IN	denotes	at
T5458	26082-26087	NN	denotes	E20.5
T5456	26088-26097	VBD	denotes	confirmed
T5459	26098-26101	DT	denotes	the
T5461	26102-26109	VBN	denotes	delayed
T5462	26110-26114	NN	denotes	lung
T5463	26115-26123	JJ	denotes	vascular
T5460	26124-26135	NN	denotes	development
T5464	26136-26138	IN	denotes	at
T5465	26139-26144	DT	denotes	these
T5467	26145-26149	JJ	denotes	late
T5468	26150-26161	JJ	denotes	gestational
T5466	26162-26168	NNS	denotes	stages
T5469	26169-26171	IN	denotes	in
T5470	26172-26177	NN	denotes	Dhcr7
T5471	26178-26187	JJ	denotes	deficient
T5472	26188-26195	NNS	denotes	embryos
T5473	26196-26197	-LRB-	denotes	(
T5475	26197-26201	NN	denotes	Fig.
T5476	26202-26203	CD	denotes	8
T5477	26203-26205	,	denotes	,
T5478	26205-26208	FW	denotes	cf.
T5479	26209-26215	NNS	denotes	panels
T5474	26216-26217	NN	denotes	G
T5480	26218-26221	CC	denotes	and
T5481	26222-26223	NN	denotes	H
T5482	26223-26224	-RRB-	denotes	)
T5483	26224-26225	.	denotes	.
T13243	26236-26250	NN	denotes	Immunostaining
T13244	26251-26259	NNS	denotes	patterns
T13245	26260-26263	IN	denotes	for
T13246	26264-26272	NN	denotes	caveolin
T13248	26272-26273	HYPH	denotes	-
T13249	26273-26274	CD	denotes	1
T13247	26274-26275	NN	denotes	α
T13250	26276-26279	CC	denotes	and
T13251	26280-26285	NN	denotes	PECAM
T13252	26285-26286	HYPH	denotes	-
T13253	26286-26287	CD	denotes	1
T13254	26288-26290	IN	denotes	in
T13255	26291-26295	NN	denotes	lung
T13256	26296-26304	NNS	denotes	sections
T13257	26304-26305	.	denotes	.
T13258	26305-26383	sentence	denotes	Cav-1α staining was compared at E14.5, E17.5 and E20.5 stages of development.
T13259	26306-26309	NN	denotes	Cav
T13261	26309-26310	HYPH	denotes	-
T13260	26310-26312	NN	denotes	1α
T13262	26313-26321	NN	denotes	staining
T13264	26322-26325	VBD	denotes	was
T13263	26326-26334	VBN	denotes	compared
T13265	26335-26337	IN	denotes	at
T13266	26338-26343	NN	denotes	E14.5
T13268	26343-26345	,	denotes	,
T13269	26345-26350	NN	denotes	E17.5
T13270	26351-26354	CC	denotes	and
T13271	26355-26360	NN	denotes	E20.5
T13267	26361-26367	NNS	denotes	stages
T13272	26368-26370	IN	denotes	of
T13273	26371-26382	NN	denotes	development
T13274	26382-26383	.	denotes	.
T13275	26383-26453	sentence	denotes	Both the epithelium and mesenchyme were negative for cav-1α at E14.5.
T13276	26384-26388	CC	denotes	Both
T13278	26389-26392	DT	denotes	the
T13277	26393-26403	NN	denotes	epithelium
T13280	26404-26407	CC	denotes	and
T13281	26408-26418	NN	denotes	mesenchyme
T13279	26419-26423	VBD	denotes	were
T13282	26424-26432	JJ	denotes	negative
T13283	26433-26436	IN	denotes	for
T13284	26437-26440	NN	denotes	cav
T13286	26440-26441	HYPH	denotes	-
T13285	26441-26443	NN	denotes	1α
T13287	26444-26446	IN	denotes	at
T13288	26447-26452	NN	denotes	E14.5
T13289	26452-26453	.	denotes	.
T13290	26453-26666	sentence	denotes	Developing blood vessels (red arrowheads) and capillaries (arrows) (panels A and B) were stained positively for cav-1α, but the staining patterns between wild-type (+/+) and knockout (-/-) lungs were not altered.
T13291	26454-26464	VBG	denotes	Developing
T13293	26465-26470	NN	denotes	blood
T13292	26471-26478	NNS	denotes	vessels
T13295	26479-26480	-LRB-	denotes	(
T13297	26480-26483	JJ	denotes	red
T13296	26484-26494	NNS	denotes	arrowheads
T13298	26494-26495	-RRB-	denotes	)
T13299	26496-26499	CC	denotes	and
T13300	26500-26511	NNS	denotes	capillaries
T13301	26512-26513	-LRB-	denotes	(
T13302	26513-26519	NNS	denotes	arrows
T13303	26519-26520	-RRB-	denotes	)
T13304	26521-26522	-LRB-	denotes	(
T13306	26522-26528	NNS	denotes	panels
T13305	26529-26530	NN	denotes	A
T13307	26531-26534	CC	denotes	and
T13308	26535-26536	NN	denotes	B
T13309	26536-26537	-RRB-	denotes	)
T13310	26538-26542	VBD	denotes	were
T13294	26543-26550	VBN	denotes	stained
T13311	26551-26561	RB	denotes	positively
T13312	26562-26565	IN	denotes	for
T13313	26566-26569	NN	denotes	cav
T13315	26569-26570	HYPH	denotes	-
T13314	26570-26572	NN	denotes	1α
T13316	26572-26574	,	denotes	,
T13317	26574-26577	CC	denotes	but
T13318	26578-26581	DT	denotes	the
T13320	26582-26590	NN	denotes	staining
T13319	26591-26599	NNS	denotes	patterns
T13322	26600-26607	IN	denotes	between
T13323	26608-26612	JJ	denotes	wild
T13325	26612-26613	HYPH	denotes	-
T13324	26613-26617	NN	denotes	type
T13327	26618-26619	-LRB-	denotes	(
T13328	26619-26620	SYM	denotes	+
T13330	26620-26621	SYM	denotes	/
T13329	26621-26622	SYM	denotes	+
T13331	26622-26623	-RRB-	denotes	)
T13332	26624-26627	CC	denotes	and
T13333	26628-26636	NN	denotes	knockout
T13334	26637-26638	-LRB-	denotes	(
T13335	26638-26639	SYM	denotes	-
T13337	26639-26640	SYM	denotes	/
T13336	26640-26641	SYM	denotes	-
T13338	26641-26642	-RRB-	denotes	)
T13326	26643-26648	NNS	denotes	lungs
T13339	26649-26653	VBD	denotes	were
T13340	26654-26657	RB	denotes	not
T13321	26658-26665	VBN	denotes	altered
T13341	26665-26666	.	denotes	.
T13342	26666-26774	sentence	denotes	Cav-1α was also detected at the proximal bronchiole sub-epithelial matrices (panels A-D, black arrowheads).
T13343	26667-26670	NN	denotes	Cav
T13345	26670-26671	HYPH	denotes	-
T13346	26671-26672	CD	denotes	1
T13344	26672-26673	NN	denotes	α
T13348	26674-26677	VBD	denotes	was
T13349	26678-26682	RB	denotes	also
T13347	26683-26691	VBN	denotes	detected
T13350	26692-26694	IN	denotes	at
T13351	26695-26698	DT	denotes	the
T13353	26699-26707	JJ	denotes	proximal
T13354	26708-26718	NN	denotes	bronchiole
T13355	26719-26733	JJ	denotes	sub-epithelial
T13352	26734-26742	NNS	denotes	matrices
T13356	26743-26744	-LRB-	denotes	(
T13358	26744-26750	NNS	denotes	panels
T13359	26751-26752	NN	denotes	A
T13360	26752-26753	SYM	denotes	-
T13361	26753-26754	NN	denotes	D
T13362	26754-26756	,	denotes	,
T13363	26756-26761	JJ	denotes	black
T13357	26762-26772	NNS	denotes	arrowheads
T13364	26772-26773	-RRB-	denotes	)
T13365	26773-26774	.	denotes	.
T13366	26774-26935	sentence	denotes	At E17.5, the airway epithelium, negative for cav-1α staining, was surrounded by rings of cav-1α positively stained capillary networks (panels C and D, arrows).
T13367	26775-26777	IN	denotes	At
T13369	26778-26783	NN	denotes	E17.5
T13370	26783-26785	,	denotes	,
T13371	26785-26788	DT	denotes	the
T13373	26789-26795	NN	denotes	airway
T13372	26796-26806	NN	denotes	epithelium
T13374	26806-26808	,	denotes	,
T13375	26808-26816	JJ	denotes	negative
T13376	26817-26820	IN	denotes	for
T13377	26821-26824	NN	denotes	cav
T13379	26824-26825	HYPH	denotes	-
T13380	26825-26826	CD	denotes	1
T13378	26826-26827	NN	denotes	α
T13381	26828-26836	NN	denotes	staining
T13382	26836-26838	,	denotes	,
T13383	26838-26841	VBD	denotes	was
T13368	26842-26852	VBN	denotes	surrounded
T13384	26853-26855	IN	denotes	by
T13385	26856-26861	NNS	denotes	rings
T13386	26862-26864	IN	denotes	of
T13387	26865-26868	NN	denotes	cav
T13389	26868-26869	HYPH	denotes	-
T13390	26869-26870	CD	denotes	1
T13388	26870-26871	NN	denotes	α
T13392	26872-26882	RB	denotes	positively
T13393	26883-26890	VBN	denotes	stained
T13394	26891-26900	JJ	denotes	capillary
T13391	26901-26909	NNS	denotes	networks
T13395	26910-26911	-LRB-	denotes	(
T13397	26911-26917	NNS	denotes	panels
T13398	26918-26919	NN	denotes	C
T13399	26920-26923	CC	denotes	and
T13400	26924-26925	NN	denotes	D
T13401	26925-26927	,	denotes	,
T13396	26927-26933	NNS	denotes	arrows
T13402	26933-26934	-RRB-	denotes	)
T13403	26934-26935	.	denotes	.
T13404	26935-27119	sentence	denotes	Cav-1α expression patterns in lungs from knockout embryos at E17.5 were similar to wild-type embryos, but relative less capillary bed was noted in knockout lungs (cf. panels C and D).
T13405	26936-26939	NN	denotes	Cav
T13407	26939-26940	HYPH	denotes	-
T13408	26940-26941	CD	denotes	1
T13406	26941-26942	NN	denotes	α
T13409	26943-26953	NN	denotes	expression
T13410	26954-26962	NNS	denotes	patterns
T13412	26963-26965	IN	denotes	in
T13413	26966-26971	NNS	denotes	lungs
T13414	26972-26976	IN	denotes	from
T13415	26977-26985	NN	denotes	knockout
T13416	26986-26993	NNS	denotes	embryos
T13417	26994-26996	IN	denotes	at
T13418	26997-27002	NN	denotes	E17.5
T13411	27003-27007	VBD	denotes	were
T13419	27008-27015	JJ	denotes	similar
T13420	27016-27018	IN	denotes	to
T13421	27019-27023	JJ	denotes	wild
T13423	27023-27024	HYPH	denotes	-
T13422	27024-27028	NN	denotes	type
T13424	27029-27036	NNS	denotes	embryos
T13425	27036-27038	,	denotes	,
T13426	27038-27041	CC	denotes	but
T13427	27042-27050	RB	denotes	relative
T13428	27051-27055	JJR	denotes	less
T13430	27056-27065	JJ	denotes	capillary
T13429	27066-27069	NN	denotes	bed
T13432	27070-27073	VBD	denotes	was
T13431	27074-27079	VBN	denotes	noted
T13433	27080-27082	IN	denotes	in
T13434	27083-27091	NN	denotes	knockout
T13435	27092-27097	NNS	denotes	lungs
T13436	27098-27099	-LRB-	denotes	(
T13438	27099-27102	FW	denotes	cf.
T13439	27103-27109	NNS	denotes	panels
T13437	27110-27111	NN	denotes	C
T13440	27112-27115	CC	denotes	and
T13441	27116-27117	NN	denotes	D
T13442	27117-27118	-RRB-	denotes	)
T13443	27118-27119	.	denotes	.
T13444	27119-27371	sentence	denotes	At E20.5 of wild-type lungs, both cav-1α and PECAM-1 staining demonstrated an extensive vascular network in the well developed saccules, with staining observed in close proximity to flat epithelial cells lining the air spaces (panels E and G, arrows).
T13445	27120-27122	IN	denotes	At
T13447	27123-27128	NN	denotes	E20.5
T13448	27129-27131	IN	denotes	of
T13449	27132-27136	JJ	denotes	wild
T13451	27136-27137	HYPH	denotes	-
T13450	27137-27141	NN	denotes	type
T13452	27142-27147	NNS	denotes	lungs
T13453	27147-27149	,	denotes	,
T13454	27149-27153	CC	denotes	both
T13456	27154-27157	NN	denotes	cav
T13457	27157-27158	HYPH	denotes	-
T13458	27158-27159	CD	denotes	1
T13455	27159-27160	NN	denotes	α
T13460	27161-27164	CC	denotes	and
T13461	27165-27170	NN	denotes	PECAM
T13462	27170-27171	HYPH	denotes	-
T13463	27171-27172	CD	denotes	1
T13459	27173-27181	NN	denotes	staining
T13446	27182-27194	VBD	denotes	demonstrated
T13464	27195-27197	DT	denotes	an
T13466	27198-27207	JJ	denotes	extensive
T13467	27208-27216	JJ	denotes	vascular
T13465	27217-27224	NN	denotes	network
T13468	27225-27227	IN	denotes	in
T13469	27228-27231	DT	denotes	the
T13471	27232-27236	RB	denotes	well
T13472	27237-27246	VBN	denotes	developed
T13470	27247-27255	NNS	denotes	saccules
T13473	27255-27257	,	denotes	,
T13474	27257-27261	IN	denotes	with
T13476	27262-27270	NN	denotes	staining
T13475	27271-27279	VBN	denotes	observed
T13477	27280-27282	IN	denotes	in
T13478	27283-27288	JJ	denotes	close
T13479	27289-27298	NN	denotes	proximity
T13480	27299-27301	IN	denotes	to
T13481	27302-27306	JJ	denotes	flat
T13483	27307-27317	JJ	denotes	epithelial
T13482	27318-27323	NNS	denotes	cells
T13484	27324-27330	VBG	denotes	lining
T13485	27331-27334	DT	denotes	the
T13487	27335-27338	NN	denotes	air
T13486	27339-27345	NNS	denotes	spaces
T13488	27346-27347	-LRB-	denotes	(
T13490	27347-27353	NNS	denotes	panels
T13491	27354-27355	NN	denotes	E
T13492	27356-27359	CC	denotes	and
T13493	27360-27361	NN	denotes	G
T13494	27361-27363	,	denotes	,
T13489	27363-27369	NNS	denotes	arrows
T13495	27369-27370	-RRB-	denotes	)
T13496	27370-27371	.	denotes	.
T13497	27371-27673	sentence	denotes	However, delayed vascular development was observed in knockout lungs, showing capillaries (panels F and H, arrows) of the knockout lungs at E20.5 were embedded in a relatively thick mesenchyme around the undeveloped epithelial tubules in which the epithelial cells were remained cuboidal (arrowheads).
T13498	27372-27379	RB	denotes	However
T13500	27379-27381	,	denotes	,
T13501	27381-27388	VBN	denotes	delayed
T13503	27389-27397	JJ	denotes	vascular
T13502	27398-27409	NN	denotes	development
T13504	27410-27413	VBD	denotes	was
T13499	27414-27422	VBN	denotes	observed
T13505	27423-27425	IN	denotes	in
T13506	27426-27434	NN	denotes	knockout
T13507	27435-27440	NNS	denotes	lungs
T13508	27440-27442	,	denotes	,
T13509	27442-27449	VBG	denotes	showing
T13510	27450-27461	NNS	denotes	capillaries
T13512	27462-27463	-LRB-	denotes	(
T13514	27463-27469	NNS	denotes	panels
T13515	27470-27471	NN	denotes	F
T13516	27472-27475	CC	denotes	and
T13517	27476-27477	NN	denotes	H
T13518	27477-27479	,	denotes	,
T13513	27479-27485	NNS	denotes	arrows
T13519	27485-27486	-RRB-	denotes	)
T13520	27487-27489	IN	denotes	of
T13521	27490-27493	DT	denotes	the
T13523	27494-27502	NN	denotes	knockout
T13522	27503-27508	NNS	denotes	lungs
T13524	27509-27511	IN	denotes	at
T13525	27512-27517	NN	denotes	E20.5
T13526	27518-27522	VBD	denotes	were
T13511	27523-27531	VBN	denotes	embedded
T13527	27532-27534	IN	denotes	in
T13528	27535-27536	DT	denotes	a
T13530	27537-27547	RB	denotes	relatively
T13531	27548-27553	JJ	denotes	thick
T13529	27554-27564	NN	denotes	mesenchyme
T13532	27565-27571	IN	denotes	around
T13533	27572-27575	DT	denotes	the
T13535	27576-27587	JJ	denotes	undeveloped
T13536	27588-27598	JJ	denotes	epithelial
T13534	27599-27606	NNS	denotes	tubules
T13537	27607-27609	IN	denotes	in
T13539	27610-27615	WDT	denotes	which
T13540	27616-27619	DT	denotes	the
T13542	27620-27630	JJ	denotes	epithelial
T13541	27631-27636	NNS	denotes	cells
T13543	27637-27641	VBD	denotes	were
T13538	27642-27650	VBN	denotes	remained
T13544	27651-27659	JJ	denotes	cuboidal
T13545	27660-27661	-LRB-	denotes	(
T13546	27661-27671	NNS	denotes	arrowheads
T13547	27671-27672	-RRB-	denotes	)
T13548	27672-27673	.	denotes	.
T13549	27673-27723	sentence	denotes	Original magnification: A-D are 20X; E-H are 40X.
T13550	27674-27682	JJ	denotes	Original
T13551	27683-27696	NN	denotes	magnification
T13553	27696-27698	:	denotes	:
T13554	27698-27699	NN	denotes	A
T13556	27699-27700	HYPH	denotes	-
T13555	27700-27701	NN	denotes	D
T13557	27702-27705	VBP	denotes	are
T13558	27706-27709	NN	denotes	20X
T13559	27709-27710	:	denotes	;
T13560	27711-27712	NN	denotes	E
T13562	27712-27713	HYPH	denotes	-
T13561	27713-27714	NN	denotes	H
T13552	27715-27718	VBP	denotes	are
T13563	27719-27722	NN	denotes	40X
T13564	27722-27723	.	denotes	.
T5726	27725-27727	VBZ	denotes	Is
T5728	27728-27731	DT	denotes	the
T5729	27732-27739	NN	denotes	pattern
T5730	27740-27742	IN	denotes	of
T5731	27743-27748	JJ	denotes	sonic
T5733	27749-27757	NN	denotes	hedgehog
T5732	27758-27768	NN	denotes	expression
T5734	27769-27771	CC	denotes	or
T5735	27772-27781	NN	denotes	signaling
T5727	27782-27789	VBN	denotes	altered
T5736	27790-27792	IN	denotes	in
T5737	27793-27798	NN	denotes	Dhcr7
T5739	27798-27799	SYM	denotes	-
T5740	27799-27800	SYM	denotes	/
T5741	27800-27801	SYM	denotes	-
T5738	27802-27809	NNS	denotes	embryos
T5742	27809-27810	.	denotes	?
T5743	27810-28024	sentence	denotes	Shh is critical for normal embryonic development and its autoprocessing, resulting in a cholesterol molecule covalently attached at its C-terminus, is a necessary step for its regulated signaling function [23,34].
T5744	27811-27814	NN	denotes	Shh
T5745	27815-27817	VBZ	denotes	is
T5746	27818-27826	JJ	denotes	critical
T5747	27827-27830	IN	denotes	for
T5748	27831-27837	JJ	denotes	normal
T5750	27838-27847	JJ	denotes	embryonic
T5749	27848-27859	NN	denotes	development
T5751	27860-27863	CC	denotes	and
T5752	27864-27867	PRP$	denotes	its
T5753	27868-27882	NN	denotes	autoprocessing
T5755	27882-27884	,	denotes	,
T5756	27884-27893	VBG	denotes	resulting
T5757	27894-27896	IN	denotes	in
T5758	27897-27898	DT	denotes	a
T5760	27899-27910	NN	denotes	cholesterol
T5759	27911-27919	NN	denotes	molecule
T5761	27920-27930	RB	denotes	covalently
T5762	27931-27939	VBN	denotes	attached
T5763	27940-27942	IN	denotes	at
T5764	27943-27946	PRP$	denotes	its
T5766	27947-27948	NN	denotes	C
T5767	27948-27949	HYPH	denotes	-
T5765	27949-27957	NN	denotes	terminus
T5768	27957-27959	,	denotes	,
T5754	27959-27961	VBZ	denotes	is
T5769	27962-27963	DT	denotes	a
T5771	27964-27973	JJ	denotes	necessary
T5770	27974-27978	NN	denotes	step
T5772	27979-27982	IN	denotes	for
T5773	27983-27986	PRP$	denotes	its
T5775	27987-27996	VBN	denotes	regulated
T5776	27997-28006	NN	denotes	signaling
T5774	28007-28015	NN	denotes	function
T5777	28016-28017	-LRB-	denotes	[
T5779	28017-28019	CD	denotes	23
T5780	28019-28020	,	denotes	,
T5778	28020-28022	CD	denotes	34
T5781	28022-28023	-RRB-	denotes	]
T5782	28023-28024	.	denotes	.
T5783	28024-28159	sentence	denotes	Thus, disruption of cholesterol biosynthesis could conceivably disrupt its pattern of distribution and thus affect normal development.
T5784	28025-28029	RB	denotes	Thus
T5786	28029-28031	,	denotes	,
T5787	28031-28041	NN	denotes	disruption
T5788	28042-28044	IN	denotes	of
T5789	28045-28056	NN	denotes	cholesterol
T5790	28057-28069	NN	denotes	biosynthesis
T5791	28070-28075	MD	denotes	could
T5792	28076-28087	RB	denotes	conceivably
T5785	28088-28095	VB	denotes	disrupt
T5793	28096-28099	PRP$	denotes	its
T5794	28100-28107	NN	denotes	pattern
T5795	28108-28110	IN	denotes	of
T5796	28111-28123	NN	denotes	distribution
T5797	28124-28127	CC	denotes	and
T5798	28128-28132	RB	denotes	thus
T5799	28133-28139	VB	denotes	affect
T5800	28140-28146	JJ	denotes	normal
T5801	28147-28158	NN	denotes	development
T5802	28158-28159	.	denotes	.
T5803	28159-28374	sentence	denotes	To determine whether aberrant expression of Shh contributed to the Dhcr7-/- lung phenotype, distribution of Shh in the Dhcr7-/- and wild-type lungs was assessed from E10.5 to P0 by IHC using Shh-N peptide antibody.
T5804	28160-28162	TO	denotes	To
T5805	28163-28172	VB	denotes	determine
T5807	28173-28180	IN	denotes	whether
T5809	28181-28189	JJ	denotes	aberrant
T5810	28190-28200	NN	denotes	expression
T5811	28201-28203	IN	denotes	of
T5812	28204-28207	NN	denotes	Shh
T5808	28208-28219	VBD	denotes	contributed
T5813	28220-28222	IN	denotes	to
T5814	28223-28226	DT	denotes	the
T5816	28227-28232	NN	denotes	Dhcr7
T5817	28232-28233	SYM	denotes	-
T5818	28233-28234	SYM	denotes	/
T5819	28234-28235	SYM	denotes	-
T5820	28236-28240	NN	denotes	lung
T5815	28241-28250	NN	denotes	phenotype
T5821	28250-28252	,	denotes	,
T5822	28252-28264	NN	denotes	distribution
T5823	28265-28267	IN	denotes	of
T5824	28268-28271	NN	denotes	Shh
T5825	28272-28274	IN	denotes	in
T5826	28275-28278	DT	denotes	the
T5828	28279-28284	NN	denotes	Dhcr7
T5829	28284-28285	SYM	denotes	-
T5830	28285-28286	SYM	denotes	/
T5831	28286-28287	SYM	denotes	-
T5832	28288-28291	CC	denotes	and
T5833	28292-28296	JJ	denotes	wild
T5835	28296-28297	HYPH	denotes	-
T5834	28297-28301	NN	denotes	type
T5827	28302-28307	NNS	denotes	lungs
T5836	28308-28311	VBD	denotes	was
T5806	28312-28320	VBN	denotes	assessed
T5837	28321-28325	IN	denotes	from
T5838	28326-28331	NN	denotes	E10.5
T5839	28332-28334	IN	denotes	to
T5840	28335-28337	NN	denotes	P0
T5841	28338-28340	IN	denotes	by
T5842	28341-28344	NN	denotes	IHC
T5843	28345-28350	VBG	denotes	using
T5844	28351-28354	NN	denotes	Shh
T5846	28354-28355	HYPH	denotes	-
T5845	28355-28356	NN	denotes	N
T5848	28357-28364	NN	denotes	peptide
T5847	28365-28373	NN	denotes	antibody
T5849	28373-28374	.	denotes	.
T5850	28374-28620	sentence	denotes	At E10.5, the distribution of Shh was detected in the epithelial cells of lung buds (Fig. 9, left panel: C and D), as well as in the more typical Shh-expressing locations, such as the floor plate, notochord and mid gut (Fig. 9, left panel: A-D).
T5851	28375-28377	IN	denotes	At
T5853	28378-28383	NN	denotes	E10.5
T5854	28383-28385	,	denotes	,
T5855	28385-28388	DT	denotes	the
T5856	28389-28401	NN	denotes	distribution
T5857	28402-28404	IN	denotes	of
T5858	28405-28408	NN	denotes	Shh
T5859	28409-28412	VBD	denotes	was
T5852	28413-28421	VBN	denotes	detected
T5860	28422-28424	IN	denotes	in
T5861	28425-28428	DT	denotes	the
T5863	28429-28439	JJ	denotes	epithelial
T5862	28440-28445	NNS	denotes	cells
T5864	28446-28448	IN	denotes	of
T5865	28449-28453	NN	denotes	lung
T5866	28454-28458	NNS	denotes	buds
T5867	28459-28460	-LRB-	denotes	(
T5869	28460-28464	NN	denotes	Fig.
T5870	28465-28466	CD	denotes	9
T5871	28466-28468	,	denotes	,
T5872	28468-28472	JJ	denotes	left
T5868	28473-28478	NN	denotes	panel
T5873	28478-28480	:	denotes	:
T5874	28480-28481	LS	denotes	C
T5875	28482-28485	CC	denotes	and
T5876	28486-28487	LS	denotes	D
T5877	28487-28488	-RRB-	denotes	)
T5878	28488-28490	,	denotes	,
T5879	28490-28492	RB	denotes	as
T5881	28493-28497	RB	denotes	well
T5880	28498-28500	IN	denotes	as
T5882	28501-28503	IN	denotes	in
T5883	28504-28507	DT	denotes	the
T5885	28508-28512	RBR	denotes	more
T5886	28513-28520	JJ	denotes	typical
T5887	28521-28524	NN	denotes	Shh
T5889	28524-28525	HYPH	denotes	-
T5888	28525-28535	VBG	denotes	expressing
T5884	28536-28545	NNS	denotes	locations
T5890	28545-28547	,	denotes	,
T5891	28547-28551	JJ	denotes	such
T5892	28552-28554	IN	denotes	as
T5893	28555-28558	DT	denotes	the
T5895	28559-28564	NN	denotes	floor
T5894	28565-28570	NN	denotes	plate
T5896	28570-28572	,	denotes	,
T5897	28572-28581	NN	denotes	notochord
T5898	28582-28585	CC	denotes	and
T5899	28586-28589	JJ	denotes	mid
T5900	28590-28593	NN	denotes	gut
T5901	28594-28595	-LRB-	denotes	(
T5903	28595-28599	NN	denotes	Fig.
T5904	28600-28601	CD	denotes	9
T5905	28601-28603	,	denotes	,
T5906	28603-28607	JJ	denotes	left
T5902	28608-28613	NN	denotes	panel
T5907	28613-28615	:	denotes	:
T5908	28615-28616	LS	denotes	A
T5909	28616-28617	SYM	denotes	-
T5910	28617-28618	LS	denotes	D
T5911	28618-28619	-RRB-	denotes	)
T5912	28619-28620	.	denotes	.
T5913	28620-28697	sentence	denotes	Importantly, no differences between Dhcr7-/- and control embryos were noted.
T5914	28621-28632	RB	denotes	Importantly
T5916	28632-28634	,	denotes	,
T5917	28634-28636	DT	denotes	no
T5918	28637-28648	NNS	denotes	differences
T5919	28649-28656	IN	denotes	between
T5920	28657-28662	NN	denotes	Dhcr7
T5922	28662-28663	SYM	denotes	-
T5923	28663-28664	SYM	denotes	/
T5924	28664-28665	SYM	denotes	-
T5925	28666-28669	CC	denotes	and
T5926	28670-28677	NN	denotes	control
T5921	28678-28685	NNS	denotes	embryos
T5927	28686-28690	VBD	denotes	were
T5915	28691-28696	VBN	denotes	noted
T5928	28696-28697	.	denotes	.
T5929	28697-28867	sentence	denotes	Likewise, examination of lung sections from increasing gestational ages through to birth showed no significant differences between wild-type and Dhcr7 deficient tissues.
T5930	28698-28706	RB	denotes	Likewise
T5932	28706-28708	,	denotes	,
T5933	28708-28719	NN	denotes	examination
T5934	28720-28722	IN	denotes	of
T5935	28723-28727	NN	denotes	lung
T5936	28728-28736	NNS	denotes	sections
T5937	28737-28741	IN	denotes	from
T5938	28742-28752	VBG	denotes	increasing
T5940	28753-28764	JJ	denotes	gestational
T5939	28765-28769	NNS	denotes	ages
T5941	28770-28777	IN	denotes	through
T5942	28778-28780	IN	denotes	to
T5943	28781-28786	NN	denotes	birth
T5931	28787-28793	VBD	denotes	showed
T5944	28794-28796	DT	denotes	no
T5946	28797-28808	JJ	denotes	significant
T5945	28809-28820	NNS	denotes	differences
T5947	28821-28828	IN	denotes	between
T5948	28829-28833	JJ	denotes	wild
T5950	28833-28834	HYPH	denotes	-
T5949	28834-28838	NN	denotes	type
T5952	28839-28842	CC	denotes	and
T5953	28843-28848	NN	denotes	Dhcr7
T5954	28849-28858	JJ	denotes	deficient
T5951	28859-28866	NNS	denotes	tissues
T5955	28866-28867	.	denotes	.
T5956	28867-28975	sentence	denotes	The patterns of staining observed were comparable to those previously reported for Shh in mouse lungs [25].
T5957	28868-28871	DT	denotes	The
T5958	28872-28880	NNS	denotes	patterns
T5960	28881-28883	IN	denotes	of
T5961	28884-28892	NN	denotes	staining
T5962	28893-28901	VBN	denotes	observed
T5959	28902-28906	VBD	denotes	were
T5963	28907-28917	JJ	denotes	comparable
T5964	28918-28920	IN	denotes	to
T5965	28921-28926	DT	denotes	those
T5966	28927-28937	RB	denotes	previously
T5967	28938-28946	VBN	denotes	reported
T5968	28947-28950	IN	denotes	for
T5969	28951-28954	NN	denotes	Shh
T5970	28955-28957	IN	denotes	in
T5971	28958-28963	NN	denotes	mouse
T5972	28964-28969	NNS	denotes	lungs
T5973	28970-28971	-LRB-	denotes	[
T5974	28971-28973	CD	denotes	25
T5975	28973-28974	-RRB-	denotes	]
T5976	28974-28975	.	denotes	.
T5977	28975-30526	sentence	denotes	Figure 9 Shh and Ptch expression patterns at different gestational stages in Dhcr7-/- and wild-type mice. Left panel shows the Shh immunostaining patterns using an antibody to Shh N-peptide at various gestational stages as indicated by the legend on the y-axis. There were no significant differences noted at any of the stages examined between wild-type (+/+) and knockout (-/-) embryos. At E10.5, Shh was detected in the floor plate (fp), notochord (nc) (A and B), mid-gut (mg) and the epithelial buds of lungs (lb) (C and D). Shh staining was restricted to epithelial cells in the distal region of the primordial tubes of lungs at E13.5 and E15.5. Note that proximal epithelial cells of primordial tubes were not immunostained for Shh (E and F, arrows). Shh was detected in the lung epithelial cells on E18.5, and mainly in the conducting airways in the neonates. Original magnifications: A~H, K and L are 10X, I and J are 20X. Right panel shows the patterns of Ptch IHC at various gestational stages. Immunostaining of Ptch from E13.5 to P 0 confirmed concordance with Shh expression and was not significantly different between wild-type and knockout embryos. Note that the proximal epithelial cells of primordial tubes were also not immunostained for Ptch at E13.5 lungs (arrows). Original magnifications: A~F, I and J are 10X. G and H are 20X. To further investigate Shh signaling in these embryos, we examined the patterns of expression of Patched (Ptch), a cognate receptor for Shh and one whose expression is also regulated by Shh signaling.
T13692	28986-28989	NN	denotes	Shh
T13694	28990-28993	CC	denotes	and
T13695	28994-28998	NN	denotes	Ptch
T13696	28999-29009	NN	denotes	expression
T13693	29010-29018	NNS	denotes	patterns
T13697	29019-29021	IN	denotes	at
T13698	29022-29031	JJ	denotes	different
T13700	29032-29043	JJ	denotes	gestational
T13699	29044-29050	NNS	denotes	stages
T13701	29051-29053	IN	denotes	in
T13702	29054-29059	NN	denotes	Dhcr7
T13704	29059-29060	SYM	denotes	-
T13705	29060-29061	SYM	denotes	/
T13706	29061-29062	SYM	denotes	-
T13707	29063-29066	CC	denotes	and
T13708	29067-29071	JJ	denotes	wild
T13710	29071-29072	HYPH	denotes	-
T13709	29072-29076	NN	denotes	type
T13703	29077-29081	NNS	denotes	mice
T13711	29081-29082	.	denotes	.
T13712	29082-29238	sentence	denotes	Left panel shows the Shh immunostaining patterns using an antibody to Shh N-peptide at various gestational stages as indicated by the legend on the y-axis.
T13713	29083-29087	JJ	denotes	Left
T13714	29088-29093	NN	denotes	panel
T13715	29094-29099	VBZ	denotes	shows
T13716	29100-29103	DT	denotes	the
T13718	29104-29107	NN	denotes	Shh
T13719	29108-29122	NN	denotes	immunostaining
T13717	29123-29131	NNS	denotes	patterns
T13720	29132-29137	VBG	denotes	using
T13721	29138-29140	DT	denotes	an
T13722	29141-29149	NN	denotes	antibody
T13723	29150-29152	IN	denotes	to
T13724	29153-29156	NN	denotes	Shh
T13726	29157-29158	NN	denotes	N
T13727	29158-29159	HYPH	denotes	-
T13725	29159-29166	NN	denotes	peptide
T13728	29167-29169	IN	denotes	at
T13729	29170-29177	JJ	denotes	various
T13731	29178-29189	JJ	denotes	gestational
T13730	29190-29196	NNS	denotes	stages
T13732	29197-29199	IN	denotes	as
T13733	29200-29209	VBN	denotes	indicated
T13734	29210-29212	IN	denotes	by
T13735	29213-29216	DT	denotes	the
T13736	29217-29223	NN	denotes	legend
T13737	29224-29226	IN	denotes	on
T13738	29227-29230	DT	denotes	the
T13740	29231-29232	NN	denotes	y
T13741	29232-29233	HYPH	denotes	-
T13739	29233-29237	NN	denotes	axis
T13742	29237-29238	.	denotes	.
T13743	29238-29364	sentence	denotes	There were no significant differences noted at any of the stages examined between wild-type (+/+) and knockout (-/-) embryos.
T13744	29239-29244	EX	denotes	There
T13745	29245-29249	VBD	denotes	were
T13746	29250-29252	DT	denotes	no
T13748	29253-29264	JJ	denotes	significant
T13747	29265-29276	NNS	denotes	differences
T13749	29277-29282	VBN	denotes	noted
T13750	29283-29285	IN	denotes	at
T13751	29286-29289	DT	denotes	any
T13752	29290-29292	IN	denotes	of
T13753	29293-29296	DT	denotes	the
T13754	29297-29303	NNS	denotes	stages
T13755	29304-29312	VBN	denotes	examined
T13756	29313-29320	IN	denotes	between
T13757	29321-29325	JJ	denotes	wild
T13759	29325-29326	HYPH	denotes	-
T13758	29326-29330	NN	denotes	type
T13761	29331-29332	-LRB-	denotes	(
T13762	29332-29333	SYM	denotes	+
T13764	29333-29334	SYM	denotes	/
T13763	29334-29335	SYM	denotes	+
T13765	29335-29336	-RRB-	denotes	)
T13766	29337-29340	CC	denotes	and
T13767	29341-29349	NN	denotes	knockout
T13768	29350-29351	-LRB-	denotes	(
T13769	29351-29352	SYM	denotes	-
T13771	29352-29353	SYM	denotes	/
T13770	29353-29354	SYM	denotes	-
T13772	29354-29355	-RRB-	denotes	)
T13760	29356-29363	NNS	denotes	embryos
T13773	29363-29364	.	denotes	.
T13774	29364-29504	sentence	denotes	At E10.5, Shh was detected in the floor plate (fp), notochord (nc) (A and B), mid-gut (mg) and the epithelial buds of lungs (lb) (C and D).
T13775	29365-29367	IN	denotes	At
T13777	29368-29373	NN	denotes	E10.5
T13778	29373-29375	,	denotes	,
T13779	29375-29378	NN	denotes	Shh
T13780	29379-29382	VBD	denotes	was
T13776	29383-29391	VBN	denotes	detected
T13781	29392-29394	IN	denotes	in
T13782	29395-29398	DT	denotes	the
T13784	29399-29404	NN	denotes	floor
T13783	29405-29410	NN	denotes	plate
T13785	29411-29412	-LRB-	denotes	(
T13786	29412-29414	NN	denotes	fp
T13787	29414-29415	-RRB-	denotes	)
T13788	29415-29417	,	denotes	,
T13789	29417-29426	NN	denotes	notochord
T13790	29427-29428	-LRB-	denotes	(
T13791	29428-29430	NN	denotes	nc
T13792	29430-29431	-RRB-	denotes	)
T13793	29432-29433	-LRB-	denotes	(
T13794	29433-29434	NN	denotes	A
T13795	29435-29438	CC	denotes	and
T13796	29439-29440	NN	denotes	B
T13797	29440-29441	-RRB-	denotes	)
T13798	29441-29443	,	denotes	,
T13799	29443-29446	JJ	denotes	mid
T13801	29446-29447	HYPH	denotes	-
T13800	29447-29450	NN	denotes	gut
T13802	29451-29452	-LRB-	denotes	(
T13803	29452-29454	NN	denotes	mg
T13804	29454-29455	-RRB-	denotes	)
T13805	29456-29459	CC	denotes	and
T13806	29460-29463	DT	denotes	the
T13808	29464-29474	JJ	denotes	epithelial
T13807	29475-29479	NNS	denotes	buds
T13809	29480-29482	IN	denotes	of
T13810	29483-29488	NNS	denotes	lungs
T13811	29489-29490	-LRB-	denotes	(
T13812	29490-29492	NN	denotes	lb
T13813	29492-29493	-RRB-	denotes	)
T13814	29494-29495	-LRB-	denotes	(
T13815	29495-29496	NN	denotes	C
T13816	29497-29500	CC	denotes	and
T13817	29501-29502	NN	denotes	D
T13818	29502-29503	-RRB-	denotes	)
T13819	29503-29504	.	denotes	.
T13820	29504-29626	sentence	denotes	Shh staining was restricted to epithelial cells in the distal region of the primordial tubes of lungs at E13.5 and E15.5.
T13821	29505-29508	NN	denotes	Shh
T13822	29509-29517	NN	denotes	staining
T13824	29518-29521	VBD	denotes	was
T13823	29522-29532	VBN	denotes	restricted
T13825	29533-29535	IN	denotes	to
T13826	29536-29546	JJ	denotes	epithelial
T13827	29547-29552	NNS	denotes	cells
T13828	29553-29555	IN	denotes	in
T13829	29556-29559	DT	denotes	the
T13831	29560-29566	JJ	denotes	distal
T13830	29567-29573	NN	denotes	region
T13832	29574-29576	IN	denotes	of
T13833	29577-29580	DT	denotes	the
T13835	29581-29591	JJ	denotes	primordial
T13834	29592-29597	NNS	denotes	tubes
T13836	29598-29600	IN	denotes	of
T13837	29601-29606	NNS	denotes	lungs
T13838	29607-29609	IN	denotes	at
T13839	29610-29615	NN	denotes	E13.5
T13840	29616-29619	CC	denotes	and
T13841	29620-29625	NN	denotes	E15.5
T13842	29625-29626	.	denotes	.
T13843	29626-29732	sentence	denotes	Note that proximal epithelial cells of primordial tubes were not immunostained for Shh (E and F, arrows).
T13844	29627-29631	VB	denotes	Note
T13845	29632-29636	IN	denotes	that
T13847	29637-29645	JJ	denotes	proximal
T13849	29646-29656	JJ	denotes	epithelial
T13848	29657-29662	NNS	denotes	cells
T13850	29663-29665	IN	denotes	of
T13851	29666-29676	JJ	denotes	primordial
T13852	29677-29682	NNS	denotes	tubes
T13853	29683-29687	VBD	denotes	were
T13854	29688-29691	RB	denotes	not
T13846	29692-29705	VBN	denotes	immunostained
T13855	29706-29709	IN	denotes	for
T13856	29710-29713	NN	denotes	Shh
T13857	29714-29715	-LRB-	denotes	(
T13859	29715-29716	NN	denotes	E
T13860	29717-29720	CC	denotes	and
T13861	29721-29722	NN	denotes	F
T13862	29722-29724	,	denotes	,
T13858	29724-29730	NNS	denotes	arrows
T13863	29730-29731	-RRB-	denotes	)
T13864	29731-29732	.	denotes	.
T13865	29732-29842	sentence	denotes	Shh was detected in the lung epithelial cells on E18.5, and mainly in the conducting airways in the neonates.
T13866	29733-29736	NN	denotes	Shh
T13868	29737-29740	VBD	denotes	was
T13867	29741-29749	VBN	denotes	detected
T13869	29750-29752	IN	denotes	in
T13870	29753-29756	DT	denotes	the
T13872	29757-29761	NN	denotes	lung
T13873	29762-29772	JJ	denotes	epithelial
T13871	29773-29778	NNS	denotes	cells
T13874	29779-29781	IN	denotes	on
T13875	29782-29787	NN	denotes	E18.5
T13876	29787-29789	,	denotes	,
T13877	29789-29792	CC	denotes	and
T13878	29793-29799	RB	denotes	mainly
T13879	29800-29802	IN	denotes	in
T13880	29803-29806	DT	denotes	the
T13882	29807-29817	VBG	denotes	conducting
T13881	29818-29825	NNS	denotes	airways
T13883	29826-29828	IN	denotes	in
T13884	29829-29832	DT	denotes	the
T13885	29833-29841	NNS	denotes	neonates
T13886	29841-29842	.	denotes	.
T13887	29842-29906	sentence	denotes	Original magnifications: A~H, K and L are 10X, I and J are 20X.
T13888	29843-29851	JJ	denotes	Original
T13889	29852-29866	NNS	denotes	magnifications
T13891	29866-29868	:	denotes	:
T13892	29868-29869	NN	denotes	A
T13894	29869-29870	SYM	denotes	~
T13895	29870-29871	NN	denotes	H
T13896	29871-29873	,	denotes	,
T13897	29873-29874	NN	denotes	K
T13898	29875-29878	CC	denotes	and
T13899	29879-29880	NN	denotes	L
T13893	29881-29884	VBP	denotes	are
T13900	29885-29888	NN	denotes	10X
T13901	29888-29890	,	denotes	,
T13902	29890-29891	NN	denotes	I
T13903	29892-29895	CC	denotes	and
T13904	29896-29897	NN	denotes	J
T13890	29898-29901	VBP	denotes	are
T13905	29902-29905	NN	denotes	20X
T13906	29905-29906	.	denotes	.
T13907	29906-29980	sentence	denotes	Right panel shows the patterns of Ptch IHC at various gestational stages.
T13908	29907-29912	JJ	denotes	Right
T13909	29913-29918	NN	denotes	panel
T13910	29919-29924	VBZ	denotes	shows
T13911	29925-29928	DT	denotes	the
T13912	29929-29937	NNS	denotes	patterns
T13913	29938-29940	IN	denotes	of
T13914	29941-29945	NN	denotes	Ptch
T13915	29946-29949	NN	denotes	IHC
T13916	29950-29952	IN	denotes	at
T13917	29953-29960	JJ	denotes	various
T13919	29961-29972	JJ	denotes	gestational
T13918	29973-29979	NNS	denotes	stages
T13920	29979-29980	.	denotes	.
T13921	29980-30139	sentence	denotes	Immunostaining of Ptch from E13.5 to P 0 confirmed concordance with Shh expression and was not significantly different between wild-type and knockout embryos.
T13922	29981-29995	NN	denotes	Immunostaining
T13924	29996-29998	IN	denotes	of
T13925	29999-30003	NN	denotes	Ptch
T13926	30004-30008	IN	denotes	from
T13927	30009-30014	NN	denotes	E13.5
T13928	30015-30017	IN	denotes	to
T13929	30018-30019	NN	denotes	P
T13930	30020-30021	CD	denotes	0
T13923	30022-30031	VBD	denotes	confirmed
T13931	30032-30043	NN	denotes	concordance
T13932	30044-30048	IN	denotes	with
T13933	30049-30052	NN	denotes	Shh
T13934	30053-30063	NN	denotes	expression
T13935	30064-30067	CC	denotes	and
T13936	30068-30071	VBD	denotes	was
T13937	30072-30075	RB	denotes	not
T13938	30076-30089	RB	denotes	significantly
T13939	30090-30099	JJ	denotes	different
T13940	30100-30107	IN	denotes	between
T13941	30108-30112	JJ	denotes	wild
T13943	30112-30113	HYPH	denotes	-
T13942	30113-30117	NN	denotes	type
T13945	30118-30121	CC	denotes	and
T13946	30122-30130	NN	denotes	knockout
T13944	30131-30138	NNS	denotes	embryos
T13947	30138-30139	.	denotes	.
T13948	30139-30261	sentence	denotes	Note that the proximal epithelial cells of primordial tubes were also not immunostained for Ptch at E13.5 lungs (arrows).
T13949	30140-30144	VB	denotes	Note
T13950	30145-30149	IN	denotes	that
T13952	30150-30153	DT	denotes	the
T13954	30154-30162	JJ	denotes	proximal
T13955	30163-30173	JJ	denotes	epithelial
T13953	30174-30179	NNS	denotes	cells
T13956	30180-30182	IN	denotes	of
T13957	30183-30193	JJ	denotes	primordial
T13958	30194-30199	NNS	denotes	tubes
T13959	30200-30204	VBD	denotes	were
T13960	30205-30209	RB	denotes	also
T13961	30210-30213	RB	denotes	not
T13951	30214-30227	VBN	denotes	immunostained
T13962	30228-30231	IN	denotes	for
T13963	30232-30236	NN	denotes	Ptch
T13964	30237-30239	IN	denotes	at
T13965	30240-30245	NN	denotes	E13.5
T13966	30246-30251	NNS	denotes	lungs
T13967	30252-30253	-LRB-	denotes	(
T13968	30253-30259	NNS	denotes	arrows
T13969	30259-30260	-RRB-	denotes	)
T13970	30260-30261	.	denotes	.
T13971	30261-30308	sentence	denotes	Original magnifications: A~F, I and J are 10X.
T13972	30262-30270	JJ	denotes	Original
T13973	30271-30285	NNS	denotes	magnifications
T13975	30285-30287	:	denotes	:
T13976	30287-30288	NN	denotes	A
T13977	30288-30289	SYM	denotes	~
T13978	30289-30290	NN	denotes	F
T13979	30290-30292	,	denotes	,
T13980	30292-30293	NN	denotes	I
T13981	30294-30297	CC	denotes	and
T13982	30298-30299	NN	denotes	J
T13974	30300-30303	VBP	denotes	are
T13983	30304-30307	NN	denotes	10X
T13984	30307-30308	.	denotes	.
T13985	30308-30325	sentence	denotes	G and H are 20X.
T13986	30309-30310	NN	denotes	G
T13988	30311-30314	CC	denotes	and
T13989	30315-30316	NN	denotes	H
T13987	30317-30320	VBP	denotes	are
T13990	30321-30324	NN	denotes	20X
T13991	30324-30325	.	denotes	.
T5978	30326-30328	TO	denotes	To
T5980	30329-30336	RB	denotes	further
T5979	30337-30348	VB	denotes	investigate
T5982	30349-30352	NN	denotes	Shh
T5983	30353-30362	NN	denotes	signaling
T5984	30363-30365	IN	denotes	in
T5985	30366-30371	DT	denotes	these
T5986	30372-30379	NNS	denotes	embryos
T5987	30379-30381	,	denotes	,
T5988	30381-30383	PRP	denotes	we
T5981	30384-30392	VBD	denotes	examined
T5989	30393-30396	DT	denotes	the
T5990	30397-30405	NNS	denotes	patterns
T5991	30406-30408	IN	denotes	of
T5992	30409-30419	NN	denotes	expression
T5993	30420-30422	IN	denotes	of
T5994	30423-30430	NN	denotes	Patched
T5995	30431-30432	-LRB-	denotes	(
T5996	30432-30436	NN	denotes	Ptch
T5997	30436-30437	-RRB-	denotes	)
T5998	30437-30439	,	denotes	,
T5999	30439-30440	DT	denotes	a
T6001	30441-30448	JJ	denotes	cognate
T6000	30449-30457	NN	denotes	receptor
T6002	30458-30461	IN	denotes	for
T6003	30462-30465	NN	denotes	Shh
T6004	30466-30469	CC	denotes	and
T6005	30470-30473	CD	denotes	one
T6006	30474-30479	WP$	denotes	whose
T6007	30480-30490	NN	denotes	expression
T6009	30491-30493	VBZ	denotes	is
T6010	30494-30498	RB	denotes	also
T6008	30499-30508	VBN	denotes	regulated
T6011	30509-30511	IN	denotes	by
T6012	30512-30515	NN	denotes	Shh
T6013	30516-30525	NN	denotes	signaling
T6014	30525-30526	.	denotes	.
T6015	30526-30687	sentence	denotes	The patterns of Ptch in lung sections from various gestational stages were also indistinguishable between wild-type and knockout embryos (Fig. 9, right panels).
T6016	30527-30530	DT	denotes	The
T6017	30531-30539	NNS	denotes	patterns
T6019	30540-30542	IN	denotes	of
T6020	30543-30547	NN	denotes	Ptch
T6021	30548-30550	IN	denotes	in
T6022	30551-30555	NN	denotes	lung
T6023	30556-30564	NNS	denotes	sections
T6024	30565-30569	IN	denotes	from
T6025	30570-30577	JJ	denotes	various
T6027	30578-30589	JJ	denotes	gestational
T6026	30590-30596	NNS	denotes	stages
T6018	30597-30601	VBD	denotes	were
T6028	30602-30606	RB	denotes	also
T6029	30607-30624	JJ	denotes	indistinguishable
T6030	30625-30632	IN	denotes	between
T6031	30633-30637	JJ	denotes	wild
T6033	30637-30638	HYPH	denotes	-
T6032	30638-30642	NN	denotes	type
T6035	30643-30646	CC	denotes	and
T6036	30647-30655	NN	denotes	knockout
T6034	30656-30663	NNS	denotes	embryos
T6037	30664-30665	-LRB-	denotes	(
T6039	30665-30669	NN	denotes	Fig.
T6040	30670-30671	CD	denotes	9
T6041	30671-30673	,	denotes	,
T6042	30673-30678	JJ	denotes	right
T6038	30679-30685	NNS	denotes	panels
T6043	30685-30686	-RRB-	denotes	)
T6044	30686-30687	.	denotes	.
T6045	30687-30819	sentence	denotes	The expression of Ptch was in concordance with that of Shh (Fig 9, left and right panels), showing lung epithelial cell expression.
T6046	30688-30691	DT	denotes	The
T6047	30692-30702	NN	denotes	expression
T6049	30703-30705	IN	denotes	of
T6050	30706-30710	NN	denotes	Ptch
T6048	30711-30714	VBD	denotes	was
T6051	30715-30717	IN	denotes	in
T6052	30718-30729	NN	denotes	concordance
T6053	30730-30734	IN	denotes	with
T6054	30735-30739	DT	denotes	that
T6055	30740-30742	IN	denotes	of
T6056	30743-30746	NN	denotes	Shh
T6057	30747-30748	-LRB-	denotes	(
T6059	30748-30751	NN	denotes	Fig
T6060	30752-30753	CD	denotes	9
T6061	30753-30755	,	denotes	,
T6062	30755-30759	JJ	denotes	left
T6063	30760-30763	CC	denotes	and
T6064	30764-30769	JJ	denotes	right
T6058	30770-30776	NNS	denotes	panels
T6065	30776-30777	-RRB-	denotes	)
T6066	30777-30779	,	denotes	,
T6067	30779-30786	VBG	denotes	showing
T6068	30787-30791	NN	denotes	lung
T6070	30792-30802	JJ	denotes	epithelial
T6069	30803-30807	NN	denotes	cell
T6071	30808-30818	NN	denotes	expression
T6072	30818-30819	.	denotes	.
T6073	30819-30992	sentence	denotes	Downstream of the Shh-Ptch signaling cascade are proteins such as smoothened (Smo), and Gli proteins, key regulators of target-gene expression in response to Shh signaling.
T6074	30820-30830	RB	denotes	Downstream
T6076	30831-30833	IN	denotes	of
T6077	30834-30837	DT	denotes	the
T6079	30838-30841	NN	denotes	Shh
T6081	30841-30842	HYPH	denotes	-
T6080	30842-30846	NN	denotes	Ptch
T6082	30847-30856	NN	denotes	signaling
T6078	30857-30864	NN	denotes	cascade
T6075	30865-30868	VBP	denotes	are
T6083	30869-30877	NN	denotes	proteins
T6084	30878-30882	JJ	denotes	such
T6085	30883-30885	IN	denotes	as
T6086	30886-30896	VBN	denotes	smoothened
T6088	30897-30898	-LRB-	denotes	(
T6089	30898-30901	NN	denotes	Smo
T6090	30901-30902	-RRB-	denotes	)
T6091	30902-30904	,	denotes	,
T6092	30904-30907	CC	denotes	and
T6093	30908-30911	NN	denotes	Gli
T6087	30912-30920	NN	denotes	proteins
T6094	30920-30922	,	denotes	,
T6095	30922-30925	JJ	denotes	key
T6096	30926-30936	NNS	denotes	regulators
T6097	30937-30939	IN	denotes	of
T6098	30940-30946	NN	denotes	target
T6100	30946-30947	HYPH	denotes	-
T6099	30947-30951	NN	denotes	gene
T6101	30952-30962	NN	denotes	expression
T6102	30963-30965	IN	denotes	in
T6103	30966-30974	NN	denotes	response
T6104	30975-30977	IN	denotes	to
T6105	30978-30981	NN	denotes	Shh
T6106	30982-30991	NN	denotes	signaling
T6107	30991-30992	.	denotes	.
T6108	30992-31218	sentence	denotes	No differences in expression patterns for Smo (Fig. 10, upper panels), Gli1 (Fig. 10, middle panels) or Gli3 (Fig. 10, bottom panels) were noted between wild-type and knockout lungs at E13.5, E15.5 and E17.5 gestational days.
T6109	30993-30995	DT	denotes	No
T6110	30996-31007	NNS	denotes	differences
T6112	31008-31010	IN	denotes	in
T6113	31011-31021	NN	denotes	expression
T6114	31022-31030	NNS	denotes	patterns
T6115	31031-31034	IN	denotes	for
T6116	31035-31038	NN	denotes	Smo
T6117	31039-31040	-LRB-	denotes	(
T6119	31040-31044	NN	denotes	Fig.
T6120	31045-31047	CD	denotes	10
T6121	31047-31049	,	denotes	,
T6122	31049-31054	JJ	denotes	upper
T6118	31055-31061	NNS	denotes	panels
T6123	31061-31062	-RRB-	denotes	)
T6124	31062-31064	,	denotes	,
T6125	31064-31068	NN	denotes	Gli1
T6126	31069-31070	-LRB-	denotes	(
T6128	31070-31074	NN	denotes	Fig.
T6129	31075-31077	CD	denotes	10
T6130	31077-31079	,	denotes	,
T6131	31079-31085	JJ	denotes	middle
T6127	31086-31092	NNS	denotes	panels
T6132	31092-31093	-RRB-	denotes	)
T6133	31094-31096	CC	denotes	or
T6134	31097-31101	NN	denotes	Gli3
T6135	31102-31103	-LRB-	denotes	(
T6137	31103-31107	NN	denotes	Fig.
T6138	31108-31110	CD	denotes	10
T6139	31110-31112	,	denotes	,
T6140	31112-31118	NN	denotes	bottom
T6136	31119-31125	NNS	denotes	panels
T6141	31125-31126	-RRB-	denotes	)
T6142	31127-31131	VBD	denotes	were
T6111	31132-31137	VBN	denotes	noted
T6143	31138-31145	IN	denotes	between
T6144	31146-31150	JJ	denotes	wild
T6146	31150-31151	HYPH	denotes	-
T6145	31151-31155	NN	denotes	type
T6148	31156-31159	CC	denotes	and
T6149	31160-31168	NN	denotes	knockout
T6147	31169-31174	NNS	denotes	lungs
T6150	31175-31177	IN	denotes	at
T6151	31178-31183	NN	denotes	E13.5
T6153	31183-31185	,	denotes	,
T6154	31185-31190	NN	denotes	E15.5
T6155	31191-31194	CC	denotes	and
T6156	31195-31200	NN	denotes	E17.5
T6157	31201-31212	JJ	denotes	gestational
T6152	31213-31217	NNS	denotes	days
T6158	31217-31218	.	denotes	.
T14030	31230-31241	NNS	denotes	Comparisons
T14031	31242-31244	IN	denotes	of
T14032	31245-31248	NN	denotes	Smo
T14034	31248-31250	,	denotes	,
T14035	31250-31254	NN	denotes	Gli1
T14036	31255-31258	CC	denotes	and
T14037	31259-31263	NN	denotes	Gli3
T14033	31264-31274	NN	denotes	expression
T14038	31275-31277	IN	denotes	in
T14039	31278-31282	NN	denotes	lung
T14040	31283-31291	NNS	denotes	sections
T14041	31291-31292	.	denotes	.
T14042	31292-31460	sentence	denotes	Smoothened (Smo, upper panel), Gli1 (middle panel) and Gli3 (bottom panel) showed a pattern of distribution similar to that seen with Shh and Ptch (compare to Fig. 7).
T14043	31293-31303	JJ	denotes	Smoothened
T14045	31304-31305	-LRB-	denotes	(
T14046	31305-31308	NN	denotes	Smo
T14047	31308-31310	,	denotes	,
T14049	31310-31315	JJ	denotes	upper
T14048	31316-31321	NN	denotes	panel
T14050	31321-31322	-RRB-	denotes	)
T14051	31322-31324	,	denotes	,
T14052	31324-31328	NN	denotes	Gli1
T14053	31329-31330	-LRB-	denotes	(
T14055	31330-31336	JJ	denotes	middle
T14054	31337-31342	NN	denotes	panel
T14056	31342-31343	-RRB-	denotes	)
T14057	31344-31347	CC	denotes	and
T14058	31348-31352	NN	denotes	Gli3
T14059	31353-31354	-LRB-	denotes	(
T14061	31354-31360	NN	denotes	bottom
T14060	31361-31366	NN	denotes	panel
T14062	31366-31367	-RRB-	denotes	)
T14044	31368-31374	VBD	denotes	showed
T14063	31375-31376	DT	denotes	a
T14064	31377-31384	NN	denotes	pattern
T14065	31385-31387	IN	denotes	of
T14066	31388-31400	NN	denotes	distribution
T14067	31401-31408	JJ	denotes	similar
T14068	31409-31411	IN	denotes	to
T14069	31412-31416	DT	denotes	that
T14070	31417-31421	VBN	denotes	seen
T14071	31422-31426	IN	denotes	with
T14072	31427-31430	NN	denotes	Shh
T14073	31431-31434	CC	denotes	and
T14074	31435-31439	NN	denotes	Ptch
T14075	31440-31441	-LRB-	denotes	(
T14076	31441-31448	VB	denotes	compare
T14077	31449-31451	IN	denotes	to
T14078	31452-31456	NN	denotes	Fig.
T14079	31457-31458	CD	denotes	7
T14080	31458-31459	-RRB-	denotes	)
T14081	31459-31460	.	denotes	.
T14082	31460-31540	sentence	denotes	No differences between wild-type (+/+) and knockout (-/-) embryos were evident.
T14083	31461-31463	DT	denotes	No
T14084	31464-31475	NNS	denotes	differences
T14086	31476-31483	IN	denotes	between
T14087	31484-31488	JJ	denotes	wild
T14089	31488-31489	HYPH	denotes	-
T14088	31489-31493	NN	denotes	type
T14091	31494-31495	-LRB-	denotes	(
T14092	31495-31496	SYM	denotes	+
T14094	31496-31497	SYM	denotes	/
T14093	31497-31498	SYM	denotes	+
T14095	31498-31499	-RRB-	denotes	)
T14096	31500-31503	CC	denotes	and
T14097	31504-31512	NN	denotes	knockout
T14098	31513-31514	-LRB-	denotes	(
T14099	31514-31515	SYM	denotes	-
T14101	31515-31516	SYM	denotes	/
T14100	31516-31517	SYM	denotes	-
T14102	31517-31518	-RRB-	denotes	)
T14090	31519-31526	NNS	denotes	embryos
T14085	31527-31531	VBD	denotes	were
T14103	31532-31539	JJ	denotes	evident
T14104	31539-31540	.	denotes	.
T14105	31540-31570	sentence	denotes	Original magnifications: 20X.
T14106	31541-31549	JJ	denotes	Original
T14107	31550-31564	NNS	denotes	magnifications
T14109	31564-31566	:	denotes	:
T14108	31566-31569	NN	denotes	20X
T14110	31569-31570	.	denotes	.
T6322	31572-31575	NN	denotes	Shh
T6324	31576-31583	NN	denotes	protein
T6323	31584-31594	NN	denotes	processing
T6325	31595-31597	IN	denotes	in
T6326	31598-31603	NN	denotes	Dhcr7
T6328	31603-31604	SYM	denotes	-
T6329	31604-31605	SYM	denotes	/
T6330	31605-31606	SYM	denotes	-
T6327	31607-31614	NNS	denotes	embryos
T6331	31614-31846	sentence	denotes	Since Shh expression, as well as some of the Shh signaling components appeared to be normal in Dhcr7-/- embryos, endogenous autoprocessing of Shh to generate a cholesterol-modified N-terminal fragment of Shh (Shh-Np) was evaluated.
T6332	31615-31620	IN	denotes	Since
T6334	31621-31624	NN	denotes	Shh
T6335	31625-31635	NN	denotes	expression
T6336	31635-31637	,	denotes	,
T6337	31637-31639	RB	denotes	as
T6339	31640-31644	RB	denotes	well
T6338	31645-31647	IN	denotes	as
T6340	31648-31652	DT	denotes	some
T6341	31653-31655	IN	denotes	of
T6342	31656-31659	DT	denotes	the
T6344	31660-31663	NN	denotes	Shh
T6345	31664-31673	NN	denotes	signaling
T6343	31674-31684	NNS	denotes	components
T6333	31685-31693	VBD	denotes	appeared
T6347	31694-31696	TO	denotes	to
T6348	31697-31699	VB	denotes	be
T6349	31700-31706	JJ	denotes	normal
T6350	31707-31709	IN	denotes	in
T6351	31710-31715	NN	denotes	Dhcr7
T6353	31715-31716	SYM	denotes	-
T6354	31716-31717	SYM	denotes	/
T6355	31717-31718	SYM	denotes	-
T6352	31719-31726	NNS	denotes	embryos
T6356	31726-31728	,	denotes	,
T6357	31728-31738	JJ	denotes	endogenous
T6358	31739-31753	NN	denotes	autoprocessing
T6359	31754-31756	IN	denotes	of
T6360	31757-31760	NN	denotes	Shh
T6361	31761-31763	TO	denotes	to
T6362	31764-31772	VB	denotes	generate
T6363	31773-31774	DT	denotes	a
T6365	31775-31786	NN	denotes	cholesterol
T6367	31786-31787	HYPH	denotes	-
T6366	31787-31795	VBN	denotes	modified
T6368	31796-31797	NN	denotes	N
T6370	31797-31798	HYPH	denotes	-
T6369	31798-31806	JJ	denotes	terminal
T6364	31807-31815	NN	denotes	fragment
T6371	31816-31818	IN	denotes	of
T6372	31819-31822	NN	denotes	Shh
T6373	31823-31824	-LRB-	denotes	(
T6374	31824-31827	NN	denotes	Shh
T6376	31827-31828	HYPH	denotes	-
T6375	31828-31830	NN	denotes	Np
T6377	31830-31831	-RRB-	denotes	)
T6378	31832-31835	VBD	denotes	was
T6346	31836-31845	VBN	denotes	evaluated
T6379	31845-31846	.	denotes	.
T6380	31846-32062	sentence	denotes	COS-1 cells, transfected with full length Shh cDNA (capable of endogenous autocleavage) or a truncated Shh cDNA (Shh-N, expression of the shorter form constitutively) were used as controls (Fig. 11, tracks 1 and 2).
T6381	31847-31850	NN	denotes	COS
T6383	31850-31851	HYPH	denotes	-
T6384	31851-31852	CD	denotes	1
T6382	31853-31858	NNS	denotes	cells
T6386	31858-31860	,	denotes	,
T6387	31860-31871	VBN	denotes	transfected
T6388	31872-31876	IN	denotes	with
T6389	31877-31881	JJ	denotes	full
T6390	31882-31888	NN	denotes	length
T6392	31889-31892	NN	denotes	Shh
T6391	31893-31897	NN	denotes	cDNA
T6393	31898-31899	-LRB-	denotes	(
T6394	31899-31906	JJ	denotes	capable
T6395	31907-31909	IN	denotes	of
T6396	31910-31920	JJ	denotes	endogenous
T6397	31921-31933	NN	denotes	autocleavage
T6398	31933-31934	-RRB-	denotes	)
T6399	31935-31937	CC	denotes	or
T6400	31938-31939	DT	denotes	a
T6402	31940-31949	VBN	denotes	truncated
T6403	31950-31953	NN	denotes	Shh
T6401	31954-31958	NN	denotes	cDNA
T6404	31959-31960	-LRB-	denotes	(
T6405	31960-31963	NN	denotes	Shh
T6407	31963-31964	HYPH	denotes	-
T6406	31964-31965	NN	denotes	N
T6408	31965-31967	,	denotes	,
T6409	31967-31977	NN	denotes	expression
T6410	31978-31980	IN	denotes	of
T6411	31981-31984	DT	denotes	the
T6413	31985-31992	JJR	denotes	shorter
T6412	31993-31997	NN	denotes	form
T6414	31998-32012	RB	denotes	constitutively
T6415	32012-32013	-RRB-	denotes	)
T6416	32014-32018	VBD	denotes	were
T6385	32019-32023	VBN	denotes	used
T6417	32024-32026	IN	denotes	as
T6418	32027-32035	NNS	denotes	controls
T6419	32036-32037	-LRB-	denotes	(
T6421	32037-32041	NN	denotes	Fig.
T6422	32042-32044	CD	denotes	11
T6423	32044-32046	,	denotes	,
T6424	32046-32052	NNS	denotes	tracks
T6420	32053-32054	CD	denotes	1
T6425	32055-32058	CC	denotes	and
T6426	32059-32060	CD	denotes	2
T6427	32060-32061	-RRB-	denotes	)
T6428	32061-32062	.	denotes	.
T6429	32062-32248	sentence	denotes	Transfection of the full-length cDNA for Shh led to the detection of an ~20 kD protein that corresponds with the cleaved and tailed Shh (Fig. 11, track 1), as previously described [35].
T6430	32063-32075	NN	denotes	Transfection
T6432	32076-32078	IN	denotes	of
T6433	32079-32082	DT	denotes	the
T6435	32083-32087	JJ	denotes	full
T6437	32087-32088	HYPH	denotes	-
T6436	32088-32094	NN	denotes	length
T6434	32095-32099	NN	denotes	cDNA
T6438	32100-32103	IN	denotes	for
T6439	32104-32107	NNP	denotes	Shh
T6431	32108-32111	VBD	denotes	led
T6440	32112-32114	IN	denotes	to
T6441	32115-32118	DT	denotes	the
T6442	32119-32128	NN	denotes	detection
T6443	32129-32131	IN	denotes	of
T6444	32132-32134	DT	denotes	an
T6446	32135-32136	SYM	denotes	~
T6447	32136-32138	CD	denotes	20
T6448	32139-32141	NN	denotes	kD
T6445	32142-32149	NN	denotes	protein
T6449	32150-32154	WDT	denotes	that
T6450	32155-32166	VBZ	denotes	corresponds
T6451	32167-32171	IN	denotes	with
T6452	32172-32175	DT	denotes	the
T6454	32176-32183	VBN	denotes	cleaved
T6455	32184-32187	CC	denotes	and
T6456	32188-32194	VBN	denotes	tailed
T6453	32195-32198	NN	denotes	Shh
T6457	32199-32200	-LRB-	denotes	(
T6459	32200-32204	NN	denotes	Fig.
T6460	32205-32207	CD	denotes	11
T6461	32207-32209	,	denotes	,
T6458	32209-32214	NN	denotes	track
T6462	32215-32216	CD	denotes	1
T6463	32216-32217	-RRB-	denotes	)
T6464	32217-32219	,	denotes	,
T6465	32219-32221	IN	denotes	as
T6467	32222-32232	RB	denotes	previously
T6466	32233-32242	VBN	denotes	described
T6468	32243-32244	-LRB-	denotes	[
T6469	32244-32246	CD	denotes	35
T6470	32246-32247	-RRB-	denotes	]
T6471	32247-32248	.	denotes	.
T6472	32248-32495	sentence	denotes	Transfection of COS cells with the constitutive truncated Shh-N cDNA led to two slower migrating bands that correspond to truncated Shh, but which are thought to be generated by differences in lipid modifications, as previously demonstrated [35].
T6473	32249-32261	NN	denotes	Transfection
T6475	32262-32264	IN	denotes	of
T6476	32265-32268	NN	denotes	COS
T6477	32269-32274	NNS	denotes	cells
T6478	32275-32279	IN	denotes	with
T6479	32280-32283	DT	denotes	the
T6481	32284-32296	JJ	denotes	constitutive
T6482	32297-32306	VBN	denotes	truncated
T6483	32307-32310	NN	denotes	Shh
T6485	32310-32311	HYPH	denotes	-
T6484	32311-32312	NN	denotes	N
T6480	32313-32317	NN	denotes	cDNA
T6474	32318-32321	VBD	denotes	led
T6486	32322-32324	IN	denotes	to
T6487	32325-32328	CD	denotes	two
T6489	32329-32335	JJR	denotes	slower
T6490	32336-32345	VBG	denotes	migrating
T6488	32346-32351	NNS	denotes	bands
T6491	32352-32356	WDT	denotes	that
T6492	32357-32367	VBP	denotes	correspond
T6493	32368-32370	IN	denotes	to
T6494	32371-32380	VBN	denotes	truncated
T6495	32381-32384	NN	denotes	Shh
T6496	32384-32386	,	denotes	,
T6497	32386-32389	CC	denotes	but
T6498	32390-32395	WDT	denotes	which
T6500	32396-32399	VBP	denotes	are
T6499	32400-32407	VBN	denotes	thought
T6501	32408-32410	TO	denotes	to
T6503	32411-32413	VB	denotes	be
T6502	32414-32423	VBN	denotes	generated
T6504	32424-32426	IN	denotes	by
T6505	32427-32438	NNS	denotes	differences
T6506	32439-32441	IN	denotes	in
T6507	32442-32447	NN	denotes	lipid
T6508	32448-32461	NNS	denotes	modifications
T6509	32461-32463	,	denotes	,
T6510	32463-32465	IN	denotes	as
T6512	32466-32476	RB	denotes	previously
T6511	32477-32489	VBN	denotes	demonstrated
T6513	32490-32491	-LRB-	denotes	[
T6514	32491-32493	CD	denotes	35
T6515	32493-32494	-RRB-	denotes	]
T6516	32494-32495	.	denotes	.
T6517	32495-32676	sentence	denotes	In tissue lysates from wild-type, Dhcr7+/- and Dhcr7-/- embryos at E11.5, a band running at the same position as Shh-Np (autocleaved and tailed) was detected in all of the lysates.
T6518	32496-32498	IN	denotes	In
T6520	32499-32505	NN	denotes	tissue
T6521	32506-32513	NNS	denotes	lysates
T6522	32514-32518	IN	denotes	from
T6523	32519-32523	JJ	denotes	wild
T6525	32523-32524	HYPH	denotes	-
T6524	32524-32528	NN	denotes	type
T6527	32528-32530	,	denotes	,
T6528	32530-32535	NN	denotes	Dhcr7
T6530	32535-32536	SYM	denotes	+
T6531	32536-32537	SYM	denotes	/
T6529	32537-32538	SYM	denotes	-
T6532	32539-32542	CC	denotes	and
T6533	32543-32548	NN	denotes	Dhcr7
T6534	32548-32549	SYM	denotes	-
T6535	32549-32550	SYM	denotes	/
T6536	32550-32551	SYM	denotes	-
T6526	32552-32559	NNS	denotes	embryos
T6537	32560-32562	IN	denotes	at
T6538	32563-32568	NN	denotes	E11.5
T6539	32568-32570	,	denotes	,
T6540	32570-32571	DT	denotes	a
T6541	32572-32576	NN	denotes	band
T6542	32577-32584	VBG	denotes	running
T6543	32585-32587	IN	denotes	at
T6544	32588-32591	DT	denotes	the
T6546	32592-32596	JJ	denotes	same
T6545	32597-32605	NN	denotes	position
T6547	32606-32608	IN	denotes	as
T6548	32609-32612	NN	denotes	Shh
T6550	32612-32613	HYPH	denotes	-
T6549	32613-32615	NN	denotes	Np
T6551	32616-32617	-LRB-	denotes	(
T6552	32617-32628	VBN	denotes	autocleaved
T6553	32629-32632	CC	denotes	and
T6554	32633-32639	VBN	denotes	tailed
T6555	32639-32640	-RRB-	denotes	)
T6556	32641-32644	VBD	denotes	was
T6519	32645-32653	VBN	denotes	detected
T6557	32654-32656	IN	denotes	in
T6558	32657-32660	DT	denotes	all
T6559	32661-32663	IN	denotes	of
T6560	32664-32667	DT	denotes	the
T6561	32668-32675	NNS	denotes	lysates
T6562	32675-32676	.	denotes	.
T6563	32676-32883	sentence	denotes	There were no distinguishable differences between Dhcr7-/- and the wild-type samples, suggesting that Shh autoprocessing occurred normally, despite an absolute deficiency of cholesterol in knockout embryos.
T6564	32677-32682	EX	denotes	There
T6565	32683-32687	VBD	denotes	were
T6566	32688-32690	DT	denotes	no
T6568	32691-32706	JJ	denotes	distinguishable
T6567	32707-32718	NNS	denotes	differences
T6569	32719-32726	IN	denotes	between
T6570	32727-32732	NN	denotes	Dhcr7
T6571	32732-32733	SYM	denotes	-
T6572	32733-32734	SYM	denotes	/
T6573	32734-32735	SYM	denotes	-
T6574	32736-32739	CC	denotes	and
T6575	32740-32743	DT	denotes	the
T6577	32744-32748	JJ	denotes	wild
T6579	32748-32749	HYPH	denotes	-
T6578	32749-32753	NN	denotes	type
T6576	32754-32761	NNS	denotes	samples
T6580	32761-32763	,	denotes	,
T6581	32763-32773	VBG	denotes	suggesting
T6582	32774-32778	IN	denotes	that
T6584	32779-32782	NN	denotes	Shh
T6585	32783-32797	NN	denotes	autoprocessing
T6583	32798-32806	VBD	denotes	occurred
T6586	32807-32815	RB	denotes	normally
T6587	32815-32817	,	denotes	,
T6588	32817-32824	IN	denotes	despite
T6589	32825-32827	DT	denotes	an
T6591	32828-32836	JJ	denotes	absolute
T6590	32837-32847	NN	denotes	deficiency
T6592	32848-32850	IN	denotes	of
T6593	32851-32862	NN	denotes	cholesterol
T6594	32863-32865	IN	denotes	in
T6595	32866-32874	JJ	denotes	knockout
T6596	32875-32882	NNS	denotes	embryos
T6597	32882-32883	.	denotes	.
T6598	32883-33064	sentence	denotes	More importantly, no band corresponding to a full-length Shh (~45 kD) was present in the lung lysates from the knockout embryos, suggesting that processing of Shh was not impaired.
T6599	32884-32888	RBR	denotes	More
T6600	32889-32900	RB	denotes	importantly
T6602	32900-32902	,	denotes	,
T6603	32902-32904	DT	denotes	no
T6604	32905-32909	NN	denotes	band
T6605	32910-32923	VBG	denotes	corresponding
T6606	32924-32926	IN	denotes	to
T6607	32927-32928	DT	denotes	a
T6609	32929-32933	JJ	denotes	full
T6611	32933-32934	HYPH	denotes	-
T6610	32934-32940	NN	denotes	length
T6608	32941-32944	NN	denotes	Shh
T6612	32945-32946	-LRB-	denotes	(
T6614	32946-32947	SYM	denotes	~
T6615	32947-32949	CD	denotes	45
T6613	32950-32952	NN	denotes	kD
T6616	32952-32953	-RRB-	denotes	)
T6601	32954-32957	VBD	denotes	was
T6617	32958-32965	JJ	denotes	present
T6618	32966-32968	IN	denotes	in
T6619	32969-32972	DT	denotes	the
T6621	32973-32977	NN	denotes	lung
T6620	32978-32985	NNS	denotes	lysates
T6622	32986-32990	IN	denotes	from
T6623	32991-32994	DT	denotes	the
T6625	32995-33003	NN	denotes	knockout
T6624	33004-33011	NNS	denotes	embryos
T6626	33011-33013	,	denotes	,
T6627	33013-33023	VBG	denotes	suggesting
T6628	33024-33028	IN	denotes	that
T6630	33029-33039	NN	denotes	processing
T6631	33040-33042	IN	denotes	of
T6632	33043-33046	NN	denotes	Shh
T6633	33047-33050	VBD	denotes	was
T6634	33051-33054	RB	denotes	not
T6629	33055-33063	VBN	denotes	impaired
T6635	33063-33064	.	denotes	.
T14213	33076-33079	NN	denotes	Shh
T14214	33080-33087	NN	denotes	protein
T14215	33088-33102	NN	denotes	autoprocessing
T14216	33103-33105	IN	denotes	in
T14217	33106-33111	NN	denotes	mouse
T14218	33112-33119	NNS	denotes	embryos
T14219	33119-33120	.	denotes	.
T14220	33120-33344	sentence	denotes	Western blotting of lysates from wild-type (track 3), heterozygous (track 4) and knockout (track 5) embryos at E11.5, or COS1 cells transfected with Shh (track 1) or Shh-N (track 2) were probed with anti-Shh-N19 antibodies.
T14221	33121-33128	NNP	denotes	Western
T14222	33129-33137	NN	denotes	blotting
T14224	33138-33140	IN	denotes	of
T14225	33141-33148	NNS	denotes	lysates
T14226	33149-33153	IN	denotes	from
T14227	33154-33158	JJ	denotes	wild
T14229	33158-33159	HYPH	denotes	-
T14228	33159-33163	NN	denotes	type
T14231	33164-33165	-LRB-	denotes	(
T14232	33165-33170	NN	denotes	track
T14233	33171-33172	CD	denotes	3
T14234	33172-33173	-RRB-	denotes	)
T14235	33173-33175	,	denotes	,
T14236	33175-33187	JJ	denotes	heterozygous
T14237	33188-33189	-LRB-	denotes	(
T14238	33189-33194	NN	denotes	track
T14239	33195-33196	CD	denotes	4
T14240	33196-33197	-RRB-	denotes	)
T14241	33198-33201	CC	denotes	and
T14242	33202-33210	NN	denotes	knockout
T14243	33211-33212	-LRB-	denotes	(
T14244	33212-33217	NN	denotes	track
T14245	33218-33219	CD	denotes	5
T14246	33219-33220	-RRB-	denotes	)
T14230	33221-33228	NNS	denotes	embryos
T14247	33229-33231	IN	denotes	at
T14248	33232-33237	NN	denotes	E11.5
T14249	33237-33239	,	denotes	,
T14250	33239-33241	CC	denotes	or
T14251	33242-33246	NN	denotes	COS1
T14252	33247-33252	NNS	denotes	cells
T14253	33253-33264	VBN	denotes	transfected
T14254	33265-33269	IN	denotes	with
T14255	33270-33273	NN	denotes	Shh
T14256	33274-33275	-LRB-	denotes	(
T14257	33275-33280	NN	denotes	track
T14258	33281-33282	CD	denotes	1
T14259	33282-33283	-RRB-	denotes	)
T14260	33284-33286	CC	denotes	or
T14261	33287-33290	NN	denotes	Shh
T14263	33290-33291	HYPH	denotes	-
T14262	33291-33292	NN	denotes	N
T14264	33293-33294	-LRB-	denotes	(
T14265	33294-33299	NN	denotes	track
T14266	33300-33301	CD	denotes	2
T14267	33301-33302	-RRB-	denotes	)
T14268	33303-33307	VBD	denotes	were
T14223	33308-33314	VBN	denotes	probed
T14269	33315-33319	IN	denotes	with
T14270	33320-33328	NN	denotes	anti-Shh
T14272	33328-33329	HYPH	denotes	-
T14271	33329-33332	NN	denotes	N19
T14273	33333-33343	NNS	denotes	antibodies
T14274	33343-33344	.	denotes	.
T14275	33344-33438	sentence	denotes	20 μ g of lysate proteins from Shh transfected COS1 cells and 75 μ g from embryos was loaded.
T14276	33345-33347	CD	denotes	20
T14278	33348-33349	NN	denotes	μ
T14277	33350-33351	NNS	denotes	g
T14280	33352-33354	IN	denotes	of
T14281	33355-33361	NN	denotes	lysate
T14282	33362-33370	NN	denotes	proteins
T14283	33371-33375	IN	denotes	from
T14284	33376-33379	NN	denotes	Shh
T14285	33380-33391	VBN	denotes	transfected
T14287	33392-33396	NN	denotes	COS1
T14286	33397-33402	NNS	denotes	cells
T14288	33403-33406	CC	denotes	and
T14289	33407-33409	CD	denotes	75
T14291	33410-33411	NN	denotes	μ
T14290	33412-33413	NNS	denotes	g
T14292	33414-33418	IN	denotes	from
T14293	33419-33426	NNS	denotes	embryos
T14294	33427-33430	VBD	denotes	was
T14279	33431-33437	VBN	denotes	loaded
T14295	33437-33438	.	denotes	.
T14296	33438-33617	sentence	denotes	Both unprocessed (Shh, upper arrow ~45 kDa, faint signal) and processed (Shh-Np, lowest arrow, strong signal, track 1) forms of Shh were detected from Shh transfected COS1 cells.
T14297	33439-33443	CC	denotes	Both
T14298	33444-33455	JJ	denotes	unprocessed
T14300	33456-33457	-LRB-	denotes	(
T14302	33457-33460	NN	denotes	Shh
T14303	33460-33462	,	denotes	,
T14304	33462-33467	JJ	denotes	upper
T14305	33468-33473	NN	denotes	arrow
T14306	33474-33475	SYM	denotes	~
T14307	33475-33477	CD	denotes	45
T14308	33478-33481	NN	denotes	kDa
T14309	33481-33483	,	denotes	,
T14310	33483-33488	JJ	denotes	faint
T14301	33489-33495	NN	denotes	signal
T14311	33495-33496	-RRB-	denotes	)
T14312	33497-33500	CC	denotes	and
T14313	33501-33510	VBN	denotes	processed
T14314	33511-33512	-LRB-	denotes	(
T14316	33512-33515	NN	denotes	Shh
T14318	33515-33516	HYPH	denotes	-
T14317	33516-33518	NN	denotes	Np
T14319	33518-33520	,	denotes	,
T14320	33520-33526	JJS	denotes	lowest
T14321	33527-33532	NN	denotes	arrow
T14322	33532-33534	,	denotes	,
T14323	33534-33540	JJ	denotes	strong
T14324	33541-33547	NN	denotes	signal
T14325	33547-33549	,	denotes	,
T14315	33549-33554	NN	denotes	track
T14326	33555-33556	CD	denotes	1
T14327	33556-33557	-RRB-	denotes	)
T14299	33558-33563	NNS	denotes	forms
T14329	33564-33566	IN	denotes	of
T14330	33567-33570	NN	denotes	Shh
T14331	33571-33575	VBD	denotes	were
T14328	33576-33584	VBN	denotes	detected
T14332	33585-33589	IN	denotes	from
T14333	33590-33593	NN	denotes	Shh
T14334	33594-33605	VBN	denotes	transfected
T14336	33606-33610	NN	denotes	COS1
T14335	33611-33616	NNS	denotes	cells
T14337	33616-33617	.	denotes	.
T14338	33617-33771	sentence	denotes	The two slower migrating bands in Shh-N transfected COS1 cells (track 2) represented Shh-N proteins with different lipid modifications at its N terminus.
T14339	33618-33621	DT	denotes	The
T14341	33622-33625	CD	denotes	two
T14342	33626-33632	JJR	denotes	slower
T14343	33633-33642	VBG	denotes	migrating
T14340	33643-33648	NNS	denotes	bands
T14345	33649-33651	IN	denotes	in
T14346	33652-33655	NN	denotes	Shh
T14348	33655-33656	HYPH	denotes	-
T14347	33656-33657	NN	denotes	N
T14349	33658-33669	VBN	denotes	transfected
T14351	33670-33674	NN	denotes	COS1
T14350	33675-33680	NNS	denotes	cells
T14352	33681-33682	-LRB-	denotes	(
T14353	33682-33687	NN	denotes	track
T14354	33688-33689	CD	denotes	2
T14355	33689-33690	-RRB-	denotes	)
T14344	33691-33702	VBD	denotes	represented
T14356	33703-33706	NN	denotes	Shh
T14358	33706-33707	HYPH	denotes	-
T14357	33707-33708	NN	denotes	N
T14359	33709-33717	NN	denotes	proteins
T14360	33718-33722	IN	denotes	with
T14361	33723-33732	JJ	denotes	different
T14363	33733-33738	NN	denotes	lipid
T14362	33739-33752	NNS	denotes	modifications
T14364	33753-33755	IN	denotes	at
T14365	33756-33759	PRP$	denotes	its
T14367	33760-33761	NN	denotes	N
T14366	33762-33770	NN	denotes	terminus
T14368	33770-33771	.	denotes	.
T14369	33771-33889	sentence	denotes	A major band running at the same position as processed Shh was detected in lysates from all the embryos (tracks 3–5).
T14370	33772-33773	DT	denotes	A
T14372	33774-33779	JJ	denotes	major
T14371	33780-33784	NN	denotes	band
T14374	33785-33792	VBG	denotes	running
T14375	33793-33795	IN	denotes	at
T14376	33796-33799	DT	denotes	the
T14378	33800-33804	JJ	denotes	same
T14377	33805-33813	NN	denotes	position
T14379	33814-33816	IN	denotes	as
T14380	33817-33826	VBN	denotes	processed
T14381	33827-33830	NN	denotes	Shh
T14382	33831-33834	VBD	denotes	was
T14373	33835-33843	VBN	denotes	detected
T14383	33844-33846	IN	denotes	in
T14384	33847-33854	NNS	denotes	lysates
T14385	33855-33859	IN	denotes	from
T14386	33860-33863	PDT	denotes	all
T14388	33864-33867	DT	denotes	the
T14387	33868-33875	NNS	denotes	embryos
T14389	33876-33877	-LRB-	denotes	(
T14391	33877-33883	NNS	denotes	tracks
T14390	33884-33885	CD	denotes	3
T14392	33885-33886	SYM	denotes	–
T14393	33886-33887	CD	denotes	5
T14394	33887-33888	-RRB-	denotes	)
T14395	33888-33889	.	denotes	.
T14396	33889-34039	sentence	denotes	Importantly, no significant amounts of unprocessed or aberrantly migrating Shh proteins were detected in lysates from knockout embryos at this stage.
T14397	33890-33901	RB	denotes	Importantly
T14399	33901-33903	,	denotes	,
T14400	33903-33905	DT	denotes	no
T14402	33906-33917	JJ	denotes	significant
T14401	33918-33925	NNS	denotes	amounts
T14403	33926-33928	IN	denotes	of
T14404	33929-33940	JJ	denotes	unprocessed
T14406	33941-33943	CC	denotes	or
T14407	33944-33954	RB	denotes	aberrantly
T14408	33955-33964	VBG	denotes	migrating
T14409	33965-33968	NN	denotes	Shh
T14405	33969-33977	NN	denotes	proteins
T14410	33978-33982	VBD	denotes	were
T14398	33983-33991	VBN	denotes	detected
T14411	33992-33994	IN	denotes	in
T14412	33995-34002	NNS	denotes	lysates
T14413	34003-34007	IN	denotes	from
T14414	34008-34016	NN	denotes	knockout
T14415	34017-34024	NNS	denotes	embryos
T14416	34025-34027	IN	denotes	at
T14417	34028-34032	DT	denotes	this
T14418	34033-34038	NN	denotes	stage
T14419	34038-34039	.	denotes	.
T7458	34052-34055	DT	denotes	The
T7459	34056-34067	NN	denotes	elucidation
T7461	34068-34072	IN	denotes	that
T7463	34073-34077	NN	denotes	SLOS
T7464	34078-34080	VBZ	denotes	is
T7462	34081-34087	VBN	denotes	caused
T7465	34088-34090	IN	denotes	by
T7466	34091-34092	DT	denotes	a
T7467	34093-34099	NN	denotes	defect
T7468	34100-34102	IN	denotes	in
T7469	34103-34105	DT	denotes	an
T7470	34106-34112	NN	denotes	enzyme
T7471	34113-34122	JJ	denotes	necessary
T7472	34123-34126	IN	denotes	for
T7473	34127-34138	NN	denotes	cholesterol
T7474	34139-34151	NN	denotes	biosynthesis
T7475	34152-34155	VBZ	denotes	has
T7460	34156-34164	VBN	denotes	improved
T7476	34165-34168	PRP$	denotes	our
T7477	34169-34182	NN	denotes	understanding
T7478	34183-34185	IN	denotes	of
T7479	34186-34195	JJ	denotes	embryonic
T7480	34196-34207	NN	denotes	development
T7481	34207-34208	.	denotes	.
T7482	34208-34405	sentence	denotes	This discovery has led to the identification of other disorders of embryonic development caused by mutations in genes encoding other enzymes in the post-squalene steps of cholesterol biosynthesis.
T7483	34209-34213	DT	denotes	This
T7484	34214-34223	NN	denotes	discovery
T7486	34224-34227	VBZ	denotes	has
T7485	34228-34231	VBN	denotes	led
T7487	34232-34234	IN	denotes	to
T7488	34235-34238	DT	denotes	the
T7489	34239-34253	NN	denotes	identification
T7490	34254-34256	IN	denotes	of
T7491	34257-34262	JJ	denotes	other
T7492	34263-34272	NNS	denotes	disorders
T7493	34273-34275	IN	denotes	of
T7494	34276-34285	JJ	denotes	embryonic
T7495	34286-34297	NN	denotes	development
T7496	34298-34304	VBN	denotes	caused
T7497	34305-34307	IN	denotes	by
T7498	34308-34317	NNS	denotes	mutations
T7499	34318-34320	IN	denotes	in
T7500	34321-34326	NNS	denotes	genes
T7501	34327-34335	VBG	denotes	encoding
T7502	34336-34341	JJ	denotes	other
T7503	34342-34349	NNS	denotes	enzymes
T7504	34350-34352	IN	denotes	in
T7505	34353-34356	DT	denotes	the
T7507	34357-34370	JJ	denotes	post-squalene
T7506	34371-34376	NNS	denotes	steps
T7508	34377-34379	IN	denotes	of
T7509	34380-34391	NN	denotes	cholesterol
T7510	34392-34404	NN	denotes	biosynthesis
T7511	34404-34405	.	denotes	.
T7512	34405-34589	sentence	denotes	Although these genetic studies now implicate the need for normal cholesterol synthesis, the mechanistic role(s) of cholesterol in normal embryonic development remains to be clarified.
T7513	34406-34414	IN	denotes	Although
T7515	34415-34420	DT	denotes	these
T7517	34421-34428	JJ	denotes	genetic
T7516	34429-34436	NNS	denotes	studies
T7518	34437-34440	RB	denotes	now
T7514	34441-34450	VBP	denotes	implicate
T7520	34451-34454	DT	denotes	the
T7521	34455-34459	NN	denotes	need
T7522	34460-34463	IN	denotes	for
T7523	34464-34470	JJ	denotes	normal
T7525	34471-34482	NN	denotes	cholesterol
T7524	34483-34492	NN	denotes	synthesis
T7526	34492-34494	,	denotes	,
T7527	34494-34497	DT	denotes	the
T7529	34498-34509	JJ	denotes	mechanistic
T7528	34510-34514	NN	denotes	role
T7530	34514-34515	-LRB-	denotes	(
T7531	34515-34516	AFX	denotes	s
T7532	34516-34517	-RRB-	denotes	)
T7533	34518-34520	IN	denotes	of
T7534	34521-34532	NN	denotes	cholesterol
T7535	34533-34535	IN	denotes	in
T7536	34536-34542	JJ	denotes	normal
T7538	34543-34552	JJ	denotes	embryonic
T7537	34553-34564	NN	denotes	development
T7519	34565-34572	VBZ	denotes	remains
T7539	34573-34575	TO	denotes	to
T7541	34576-34578	VB	denotes	be
T7540	34579-34588	VBN	denotes	clarified
T7542	34588-34589	.	denotes	.
T7543	34589-34683	sentence	denotes	In the present study, we report the characterization of lung development in Dhcr7-/- embryos.
T7544	34590-34592	IN	denotes	In
T7546	34593-34596	DT	denotes	the
T7548	34597-34604	JJ	denotes	present
T7547	34605-34610	NN	denotes	study
T7549	34610-34612	,	denotes	,
T7550	34612-34614	PRP	denotes	we
T7545	34615-34621	VBP	denotes	report
T7551	34622-34625	DT	denotes	the
T7552	34626-34642	NN	denotes	characterization
T7553	34643-34645	IN	denotes	of
T7554	34646-34650	NN	denotes	lung
T7555	34651-34662	NN	denotes	development
T7556	34663-34665	IN	denotes	in
T7557	34666-34671	NN	denotes	Dhcr7
T7559	34671-34672	SYM	denotes	-
T7560	34672-34673	SYM	denotes	/
T7561	34673-34674	SYM	denotes	-
T7558	34675-34682	NNS	denotes	embryos
T7562	34682-34683	.	denotes	.
T7563	34683-34843	sentence	denotes	Although cardiac abnormalities are common in human SLOS [36], no structural abnormalities of the heart or the great vessels were observed in the Dhcr7-/- pups.
T7564	34684-34692	IN	denotes	Although
T7566	34693-34700	JJ	denotes	cardiac
T7567	34701-34714	NNS	denotes	abnormalities
T7565	34715-34718	VBP	denotes	are
T7569	34719-34725	JJ	denotes	common
T7570	34726-34728	IN	denotes	in
T7571	34729-34734	JJ	denotes	human
T7572	34735-34739	NN	denotes	SLOS
T7573	34740-34741	-LRB-	denotes	[
T7574	34741-34743	CD	denotes	36
T7575	34743-34744	-RRB-	denotes	]
T7576	34744-34746	,	denotes	,
T7577	34746-34748	DT	denotes	no
T7579	34749-34759	JJ	denotes	structural
T7578	34760-34773	NNS	denotes	abnormalities
T7580	34774-34776	IN	denotes	of
T7581	34777-34780	DT	denotes	the
T7582	34781-34786	NN	denotes	heart
T7583	34787-34789	CC	denotes	or
T7584	34790-34793	DT	denotes	the
T7586	34794-34799	JJ	denotes	great
T7585	34800-34807	NNS	denotes	vessels
T7587	34808-34812	VBD	denotes	were
T7568	34813-34821	VBN	denotes	observed
T7588	34822-34824	IN	denotes	in
T7589	34825-34828	DT	denotes	the
T7591	34829-34834	NN	denotes	Dhcr7
T7592	34834-34835	SYM	denotes	-
T7593	34835-34836	SYM	denotes	/
T7594	34836-34837	SYM	denotes	-
T7590	34838-34842	NNS	denotes	pups
T7595	34842-34843	.	denotes	.
T7596	34843-34988	sentence	denotes	Lung development in Dhcr7-/- embryos at the early stages, from lung bud formation to canalicular stage, was morphologically similar to controls.
T7597	34844-34848	NN	denotes	Lung
T7598	34849-34860	NN	denotes	development
T7600	34861-34863	IN	denotes	in
T7601	34864-34869	NN	denotes	Dhcr7
T7603	34869-34870	SYM	denotes	-
T7604	34870-34871	SYM	denotes	/
T7605	34871-34872	SYM	denotes	-
T7602	34873-34880	NNS	denotes	embryos
T7606	34881-34883	IN	denotes	at
T7607	34884-34887	DT	denotes	the
T7609	34888-34893	JJ	denotes	early
T7608	34894-34900	NNS	denotes	stages
T7610	34900-34902	,	denotes	,
T7611	34902-34906	IN	denotes	from
T7612	34907-34911	NN	denotes	lung
T7613	34912-34915	NN	denotes	bud
T7614	34916-34925	NN	denotes	formation
T7615	34926-34928	IN	denotes	to
T7616	34929-34940	JJ	denotes	canalicular
T7617	34941-34946	NN	denotes	stage
T7618	34946-34948	,	denotes	,
T7599	34948-34951	VBD	denotes	was
T7619	34952-34967	RB	denotes	morphologically
T7620	34968-34975	JJ	denotes	similar
T7621	34976-34978	IN	denotes	to
T7622	34979-34987	NNS	denotes	controls
T7623	34987-34988	.	denotes	.
T7624	34988-35295	sentence	denotes	Instead, a distinct lung hypoplasia at the saccular stage, characterized by arrested or partially developed distal epithelial tubules, reduced terminal sac space, delayed type I AECs differentiation and delayed vascular development, was consistently observed at late gestational stages in Dhcr7-/- embryos.
T7625	34989-34996	RB	denotes	Instead
T7627	34996-34998	,	denotes	,
T7628	34998-34999	DT	denotes	a
T7630	35000-35008	JJ	denotes	distinct
T7631	35009-35013	NN	denotes	lung
T7629	35014-35024	NN	denotes	hypoplasia
T7632	35025-35027	IN	denotes	at
T7633	35028-35031	DT	denotes	the
T7635	35032-35040	JJ	denotes	saccular
T7634	35041-35046	NN	denotes	stage
T7636	35046-35048	,	denotes	,
T7637	35048-35061	VBN	denotes	characterized
T7638	35062-35064	IN	denotes	by
T7639	35065-35073	VBN	denotes	arrested
T7641	35074-35076	CC	denotes	or
T7642	35077-35086	RB	denotes	partially
T7643	35087-35096	VBN	denotes	developed
T7644	35097-35103	JJ	denotes	distal
T7645	35104-35114	JJ	denotes	epithelial
T7640	35115-35122	NNS	denotes	tubules
T7646	35122-35124	,	denotes	,
T7647	35124-35131	VBN	denotes	reduced
T7649	35132-35140	JJ	denotes	terminal
T7650	35141-35144	NN	denotes	sac
T7648	35145-35150	NN	denotes	space
T7651	35150-35152	,	denotes	,
T7652	35152-35159	VBN	denotes	delayed
T7654	35160-35164	NN	denotes	type
T7655	35165-35166	CD	denotes	I
T7656	35167-35171	NNS	denotes	AECs
T7653	35172-35187	NN	denotes	differentiation
T7657	35188-35191	CC	denotes	and
T7658	35192-35199	VBN	denotes	delayed
T7660	35200-35208	JJ	denotes	vascular
T7659	35209-35220	NN	denotes	development
T7661	35220-35222	,	denotes	,
T7662	35222-35225	VBD	denotes	was
T7663	35226-35238	RB	denotes	consistently
T7626	35239-35247	VBN	denotes	observed
T7664	35248-35250	IN	denotes	at
T7665	35251-35255	JJ	denotes	late
T7667	35256-35267	JJ	denotes	gestational
T7666	35268-35274	NNS	denotes	stages
T7668	35275-35277	IN	denotes	in
T7669	35278-35283	NN	denotes	Dhcr7
T7671	35283-35284	SYM	denotes	-
T7672	35284-35285	SYM	denotes	/
T7673	35285-35286	SYM	denotes	-
T7670	35287-35294	NNS	denotes	embryos
T7674	35294-35295	.	denotes	.
T7675	35295-35499	sentence	denotes	At late stages of normal fetal development, the mammalian alveolar epithelium undergoes an abrupt differentiation as a part of the preparation of the lungs for the postnatal demands of gas exchange [37].
T7676	35296-35298	IN	denotes	At
T7678	35299-35303	JJ	denotes	late
T7679	35304-35310	NNS	denotes	stages
T7680	35311-35313	IN	denotes	of
T7681	35314-35320	JJ	denotes	normal
T7683	35321-35326	JJ	denotes	fetal
T7682	35327-35338	NN	denotes	development
T7684	35338-35340	,	denotes	,
T7685	35340-35343	DT	denotes	the
T7687	35344-35353	JJ	denotes	mammalian
T7688	35354-35362	JJ	denotes	alveolar
T7686	35363-35373	NN	denotes	epithelium
T7677	35374-35383	VBZ	denotes	undergoes
T7689	35384-35386	DT	denotes	an
T7691	35387-35393	JJ	denotes	abrupt
T7690	35394-35409	NN	denotes	differentiation
T7692	35410-35412	IN	denotes	as
T7693	35413-35414	DT	denotes	a
T7694	35415-35419	NN	denotes	part
T7695	35420-35422	IN	denotes	of
T7696	35423-35426	DT	denotes	the
T7697	35427-35438	NN	denotes	preparation
T7698	35439-35441	IN	denotes	of
T7699	35442-35445	DT	denotes	the
T7700	35446-35451	NNS	denotes	lungs
T7701	35452-35455	IN	denotes	for
T7702	35456-35459	DT	denotes	the
T7704	35460-35469	JJ	denotes	postnatal
T7703	35470-35477	NNS	denotes	demands
T7705	35478-35480	IN	denotes	of
T7706	35481-35484	NN	denotes	gas
T7707	35485-35493	NN	denotes	exchange
T7708	35494-35495	-LRB-	denotes	[
T7709	35495-35497	CD	denotes	37
T7710	35497-35498	-RRB-	denotes	]
T7711	35498-35499	.	denotes	.
T7712	35499-35636	sentence	denotes	Both type II AECs, producing pulmonary surfactants, and type I AECs, lining the expanded alveoli, are important for alveolar maturation.
T7713	35500-35504	CC	denotes	Both
T7715	35505-35509	NN	denotes	type
T7716	35510-35512	CD	denotes	II
T7714	35513-35517	NNS	denotes	AECs
T7718	35517-35519	,	denotes	,
T7719	35519-35528	VBG	denotes	producing
T7720	35529-35538	JJ	denotes	pulmonary
T7721	35539-35550	NNS	denotes	surfactants
T7722	35550-35552	,	denotes	,
T7723	35552-35555	CC	denotes	and
T7724	35556-35560	NN	denotes	type
T7726	35561-35562	CD	denotes	I
T7725	35563-35567	NNS	denotes	AECs
T7727	35567-35569	,	denotes	,
T7728	35569-35575	VBG	denotes	lining
T7729	35576-35579	DT	denotes	the
T7731	35580-35588	VBN	denotes	expanded
T7730	35589-35596	NNS	denotes	alveoli
T7732	35596-35598	,	denotes	,
T7717	35598-35601	VBP	denotes	are
T7733	35602-35611	JJ	denotes	important
T7734	35612-35615	IN	denotes	for
T7735	35616-35624	JJ	denotes	alveolar
T7736	35625-35635	NN	denotes	maturation
T7737	35635-35636	.	denotes	.
T7738	35636-35766	sentence	denotes	In addition, the vascular development of alveoli is also essential for normal lung morphogenesis at late gestational stages [19].
T7739	35637-35639	IN	denotes	In
T7741	35640-35648	NN	denotes	addition
T7742	35648-35650	,	denotes	,
T7743	35650-35653	DT	denotes	the
T7745	35654-35662	JJ	denotes	vascular
T7744	35663-35674	NN	denotes	development
T7746	35675-35677	IN	denotes	of
T7747	35678-35685	NNS	denotes	alveoli
T7740	35686-35688	VBZ	denotes	is
T7748	35689-35693	RB	denotes	also
T7749	35694-35703	JJ	denotes	essential
T7750	35704-35707	IN	denotes	for
T7751	35708-35714	JJ	denotes	normal
T7753	35715-35719	NN	denotes	lung
T7752	35720-35733	NN	denotes	morphogenesis
T7754	35734-35736	IN	denotes	at
T7755	35737-35741	JJ	denotes	late
T7757	35742-35753	JJ	denotes	gestational
T7756	35754-35760	NNS	denotes	stages
T7758	35761-35762	-LRB-	denotes	[
T7759	35762-35764	CD	denotes	19
T7760	35764-35765	-RRB-	denotes	]
T7761	35765-35766	.	denotes	.
T7762	35766-35994	sentence	denotes	We show here that the differentiation of type I AECs but not type II AECs in Dhcr7-/- lungs was blocked or delayed, as indicated by fewer flattened type I cells and by reduced expression of markers of type I AECs, T1α and AQP5.
T7763	35767-35769	PRP	denotes	We
T7764	35770-35774	VBP	denotes	show
T7765	35775-35779	RB	denotes	here
T7766	35780-35784	IN	denotes	that
T7768	35785-35788	DT	denotes	the
T7769	35789-35804	NN	denotes	differentiation
T7770	35805-35807	IN	denotes	of
T7771	35808-35812	NN	denotes	type
T7773	35813-35814	CD	denotes	I
T7772	35815-35819	NNS	denotes	AECs
T7774	35820-35823	CC	denotes	but
T7775	35824-35827	RB	denotes	not
T7776	35828-35832	NN	denotes	type
T7778	35833-35835	CD	denotes	II
T7777	35836-35840	NNS	denotes	AECs
T7779	35841-35843	IN	denotes	in
T7780	35844-35849	NN	denotes	Dhcr7
T7782	35849-35850	SYM	denotes	-
T7783	35850-35851	SYM	denotes	/
T7784	35851-35852	SYM	denotes	-
T7781	35853-35858	NNS	denotes	lungs
T7785	35859-35862	VBD	denotes	was
T7767	35863-35870	VBN	denotes	blocked
T7786	35871-35873	CC	denotes	or
T7787	35874-35881	VBN	denotes	delayed
T7788	35881-35883	,	denotes	,
T7789	35883-35885	IN	denotes	as
T7790	35886-35895	VBN	denotes	indicated
T7791	35896-35898	IN	denotes	by
T7792	35899-35904	JJR	denotes	fewer
T7794	35905-35914	VBN	denotes	flattened
T7795	35915-35919	NN	denotes	type
T7796	35920-35921	CD	denotes	I
T7793	35922-35927	NNS	denotes	cells
T7797	35928-35931	CC	denotes	and
T7798	35932-35934	IN	denotes	by
T7799	35935-35942	VBN	denotes	reduced
T7800	35943-35953	NN	denotes	expression
T7801	35954-35956	IN	denotes	of
T7802	35957-35964	NNS	denotes	markers
T7803	35965-35967	IN	denotes	of
T7804	35968-35972	NN	denotes	type
T7806	35973-35974	CD	denotes	I
T7805	35975-35979	NNS	denotes	AECs
T7807	35979-35981	,	denotes	,
T7808	35981-35984	NN	denotes	T1α
T7809	35985-35988	CC	denotes	and
T7810	35989-35993	NN	denotes	AQP5
T7811	35993-35994	.	denotes	.
T7812	35994-36087	sentence	denotes	Delayed vascular development was also involved in the delayed sacculation of Dhcr7-/- lungs.
T7813	35995-36002	VBN	denotes	Delayed
T7815	36003-36011	JJ	denotes	vascular
T7814	36012-36023	NN	denotes	development
T7817	36024-36027	VBD	denotes	was
T7818	36028-36032	RB	denotes	also
T7816	36033-36041	VBN	denotes	involved
T7819	36042-36044	IN	denotes	in
T7820	36045-36048	DT	denotes	the
T7822	36049-36056	VBN	denotes	delayed
T7821	36057-36068	NN	denotes	sacculation
T7823	36069-36071	IN	denotes	of
T7824	36072-36077	NN	denotes	Dhcr7
T7826	36077-36078	SYM	denotes	-
T7827	36078-36079	SYM	denotes	/
T7828	36079-36080	SYM	denotes	-
T7825	36081-36086	NNS	denotes	lungs
T7829	36086-36087	.	denotes	.
T7830	36087-36179	sentence	denotes	Additionally, the mesenchyme separating the AECs from the vascular beds remained thickened.
T7831	36088-36100	RB	denotes	Additionally
T7833	36100-36102	,	denotes	,
T7834	36102-36105	DT	denotes	the
T7835	36106-36116	NN	denotes	mesenchyme
T7836	36117-36127	VBG	denotes	separating
T7837	36128-36131	DT	denotes	the
T7838	36132-36136	NNS	denotes	AECs
T7839	36137-36141	IN	denotes	from
T7840	36142-36145	DT	denotes	the
T7842	36146-36154	JJ	denotes	vascular
T7841	36155-36159	NNS	denotes	beds
T7832	36160-36168	VBD	denotes	remained
T7843	36169-36178	JJ	denotes	thickened
T7844	36178-36179	.	denotes	.
T7845	36179-36411	sentence	denotes	In support of normal differentiation of type II AECs, no alteration in the gene expression patterns for surfactant proteins A, B, C or D was noted, nor was the pattern of distribution of SP-C altered in lungs from knockout embryos.
T7846	36180-36182	IN	denotes	In
T7848	36183-36190	NN	denotes	support
T7849	36191-36193	IN	denotes	of
T7850	36194-36200	JJ	denotes	normal
T7851	36201-36216	NN	denotes	differentiation
T7852	36217-36219	IN	denotes	of
T7853	36220-36224	NN	denotes	type
T7855	36225-36227	CD	denotes	II
T7854	36228-36232	NNS	denotes	AECs
T7856	36232-36234	,	denotes	,
T7857	36234-36236	DT	denotes	no
T7858	36237-36247	NN	denotes	alteration
T7859	36248-36250	IN	denotes	in
T7860	36251-36254	DT	denotes	the
T7862	36255-36259	NN	denotes	gene
T7863	36260-36270	NN	denotes	expression
T7861	36271-36279	NNS	denotes	patterns
T7864	36280-36283	IN	denotes	for
T7865	36284-36294	NN	denotes	surfactant
T7867	36295-36303	NN	denotes	proteins
T7866	36304-36305	NN	denotes	A
T7868	36305-36307	,	denotes	,
T7869	36307-36308	NN	denotes	B
T7870	36308-36310	,	denotes	,
T7871	36310-36311	NN	denotes	C
T7872	36312-36314	CC	denotes	or
T7873	36315-36316	NN	denotes	D
T7874	36317-36320	VBD	denotes	was
T7847	36321-36326	VBN	denotes	noted
T7875	36326-36328	,	denotes	,
T7876	36328-36331	CC	denotes	nor
T7877	36332-36335	VBD	denotes	was
T7879	36336-36339	DT	denotes	the
T7880	36340-36347	NN	denotes	pattern
T7881	36348-36350	IN	denotes	of
T7882	36351-36363	NN	denotes	distribution
T7883	36364-36366	IN	denotes	of
T7884	36367-36369	NN	denotes	SP
T7886	36369-36370	HYPH	denotes	-
T7885	36370-36371	NN	denotes	C
T7878	36372-36379	VBN	denotes	altered
T7887	36380-36382	IN	denotes	in
T7888	36383-36388	NNS	denotes	lungs
T7889	36389-36393	IN	denotes	from
T7890	36394-36402	NN	denotes	knockout
T7891	36403-36410	NNS	denotes	embryos
T7892	36410-36411	.	denotes	.
T7893	36411-36546	sentence	denotes	Thus, impaired gas exchange, as opposed to poor inflation and alveolar tension, may be the cause of respiratory failure in these pups.
T7894	36412-36416	RB	denotes	Thus
T7896	36416-36418	,	denotes	,
T7897	36418-36426	JJ	denotes	impaired
T7899	36427-36430	NN	denotes	gas
T7898	36431-36439	NN	denotes	exchange
T7900	36439-36441	,	denotes	,
T7901	36441-36443	IN	denotes	as
T7902	36444-36451	VBN	denotes	opposed
T7903	36452-36454	IN	denotes	to
T7904	36455-36459	JJ	denotes	poor
T7905	36460-36469	NN	denotes	inflation
T7906	36470-36473	CC	denotes	and
T7907	36474-36482	JJ	denotes	alveolar
T7908	36483-36490	NN	denotes	tension
T7909	36490-36492	,	denotes	,
T7910	36492-36495	MD	denotes	may
T7895	36496-36498	VB	denotes	be
T7911	36499-36502	DT	denotes	the
T7912	36503-36508	NN	denotes	cause
T7913	36509-36511	IN	denotes	of
T7914	36512-36523	JJ	denotes	respiratory
T7915	36524-36531	NN	denotes	failure
T7916	36532-36534	IN	denotes	in
T7917	36535-36540	DT	denotes	these
T7918	36541-36545	NNS	denotes	pups
T7919	36545-36546	.	denotes	.
T7920	36546-36638	sentence	denotes	Loss of functional Dhcr7 led to a retardation of embryonic growth from E14.5~E16.5 onwards.
T7921	36547-36551	NN	denotes	Loss
T7923	36552-36554	IN	denotes	of
T7924	36555-36565	JJ	denotes	functional
T7925	36566-36571	NN	denotes	Dhcr7
T7922	36572-36575	VBD	denotes	led
T7926	36576-36578	IN	denotes	to
T7927	36579-36580	DT	denotes	a
T7928	36581-36592	NN	denotes	retardation
T7929	36593-36595	IN	denotes	of
T7930	36596-36605	JJ	denotes	embryonic
T7931	36606-36612	NN	denotes	growth
T7932	36613-36617	IN	denotes	from
T7933	36618-36623	NN	denotes	E14.5
T7934	36623-36624	SYM	denotes	~
T7935	36624-36629	NN	denotes	E16.5
T7936	36630-36637	RB	denotes	onwards
T7937	36637-36638	.	denotes	.
T7938	36638-36800	sentence	denotes	By this stage, cholesterol levels were already lower and its immediate precursor, 7-DHC, elevated in the lungs (this study) as well as in the whole body [16,17].
T7939	36639-36641	IN	denotes	By
T7941	36642-36646	DT	denotes	this
T7942	36647-36652	NN	denotes	stage
T7943	36652-36654	,	denotes	,
T7944	36654-36665	NN	denotes	cholesterol
T7945	36666-36672	NNS	denotes	levels
T7940	36673-36677	VBD	denotes	were
T7946	36678-36685	RB	denotes	already
T7947	36686-36691	JJR	denotes	lower
T7948	36692-36695	CC	denotes	and
T7949	36696-36699	PRP$	denotes	its
T7951	36700-36709	JJ	denotes	immediate
T7950	36710-36719	NN	denotes	precursor
T7953	36719-36721	,	denotes	,
T7954	36721-36722	CD	denotes	7
T7956	36722-36723	HYPH	denotes	-
T7955	36723-36726	NN	denotes	DHC
T7957	36726-36728	,	denotes	,
T7952	36728-36736	VBN	denotes	elevated
T7958	36737-36739	IN	denotes	in
T7959	36740-36743	DT	denotes	the
T7960	36744-36749	NNS	denotes	lungs
T7961	36750-36751	-LRB-	denotes	(
T7963	36751-36755	DT	denotes	this
T7962	36756-36761	NN	denotes	study
T7964	36761-36762	-RRB-	denotes	)
T7965	36763-36765	RB	denotes	as
T7967	36766-36770	RB	denotes	well
T7966	36771-36773	IN	denotes	as
T7968	36774-36776	IN	denotes	in
T7969	36777-36780	DT	denotes	the
T7971	36781-36786	JJ	denotes	whole
T7970	36787-36791	NN	denotes	body
T7972	36792-36793	-LRB-	denotes	[
T7974	36793-36795	CD	denotes	16
T7975	36795-36796	,	denotes	,
T7973	36796-36798	CD	denotes	17
T7976	36798-36799	-RRB-	denotes	]
T7977	36799-36800	.	denotes	.
T7978	36800-36940	sentence	denotes	Intrauterine growth retardation (IUGR) followed by postnatal failure to thrive is a universally observed phenotype in SLOS patients [2,18].
T7979	36801-36813	JJ	denotes	Intrauterine
T7981	36814-36820	NN	denotes	growth
T7980	36821-36832	NN	denotes	retardation
T7983	36833-36834	-LRB-	denotes	(
T7984	36834-36838	NN	denotes	IUGR
T7985	36838-36839	-RRB-	denotes	)
T7986	36840-36848	VBN	denotes	followed
T7987	36849-36851	IN	denotes	by
T7988	36852-36861	JJ	denotes	postnatal
T7989	36862-36869	NN	denotes	failure
T7990	36870-36872	TO	denotes	to
T7991	36873-36879	VB	denotes	thrive
T7982	36880-36882	VBZ	denotes	is
T7992	36883-36884	DT	denotes	a
T7994	36885-36896	RB	denotes	universally
T7995	36897-36905	VBN	denotes	observed
T7993	36906-36915	NN	denotes	phenotype
T7996	36916-36918	IN	denotes	in
T7997	36919-36923	NN	denotes	SLOS
T7998	36924-36932	NNS	denotes	patients
T7999	36933-36934	-LRB-	denotes	[
T8001	36934-36935	CD	denotes	2
T8002	36935-36936	,	denotes	,
T8000	36936-36938	CD	denotes	18
T8003	36938-36939	-RRB-	denotes	]
T8004	36939-36940	.	denotes	.
T8005	36940-37070	sentence	denotes	We showed here that body length was significantly reduced at later stages of gestation in Dhcr7-/- animals, compared to controls.
T8006	36941-36943	PRP	denotes	We
T8007	36944-36950	VBD	denotes	showed
T8008	36951-36955	RB	denotes	here
T8009	36956-36960	IN	denotes	that
T8011	36961-36965	NN	denotes	body
T8012	36966-36972	NN	denotes	length
T8013	36973-36976	VBD	denotes	was
T8014	36977-36990	RB	denotes	significantly
T8010	36991-36998	VBN	denotes	reduced
T8015	36999-37001	IN	denotes	at
T8016	37002-37007	JJ	denotes	later
T8017	37008-37014	NNS	denotes	stages
T8018	37015-37017	IN	denotes	of
T8019	37018-37027	NN	denotes	gestation
T8020	37028-37030	IN	denotes	in
T8021	37031-37036	NN	denotes	Dhcr7
T8023	37036-37037	SYM	denotes	-
T8024	37037-37038	SYM	denotes	/
T8025	37038-37039	SYM	denotes	-
T8022	37040-37047	NNS	denotes	animals
T8026	37047-37049	,	denotes	,
T8027	37049-37057	VBN	denotes	compared
T8028	37058-37060	IN	denotes	to
T8029	37061-37069	NNS	denotes	controls
T8030	37069-37070	.	denotes	.
T8031	37070-37229	sentence	denotes	Whole body weights and fresh lung weights (as well as brain, heart and kidney, data not shown) at late gestational stages were also lower in Dhcr7-/- embryos.
T8032	37071-37076	JJ	denotes	Whole
T8033	37077-37081	NN	denotes	body
T8034	37082-37089	NNS	denotes	weights
T8036	37090-37093	CC	denotes	and
T8037	37094-37099	JJ	denotes	fresh
T8038	37100-37104	NN	denotes	lung
T8039	37105-37112	NNS	denotes	weights
T8040	37113-37114	-LRB-	denotes	(
T8041	37114-37116	RB	denotes	as
T8043	37117-37121	RB	denotes	well
T8042	37122-37124	IN	denotes	as
T8044	37125-37130	NN	denotes	brain
T8045	37130-37132	,	denotes	,
T8046	37132-37137	NN	denotes	heart
T8047	37138-37141	CC	denotes	and
T8048	37142-37148	NN	denotes	kidney
T8049	37148-37150	,	denotes	,
T8051	37150-37154	NNS	denotes	data
T8052	37155-37158	RB	denotes	not
T8050	37159-37164	VBN	denotes	shown
T8053	37164-37165	-RRB-	denotes	)
T8054	37166-37168	IN	denotes	at
T8055	37169-37173	JJ	denotes	late
T8057	37174-37185	JJ	denotes	gestational
T8056	37186-37192	NNS	denotes	stages
T8035	37193-37197	VBD	denotes	were
T8058	37198-37202	RB	denotes	also
T8059	37203-37208	JJR	denotes	lower
T8060	37209-37211	IN	denotes	in
T8061	37212-37217	NN	denotes	Dhcr7
T8063	37217-37218	SYM	denotes	-
T8064	37218-37219	SYM	denotes	/
T8065	37219-37220	SYM	denotes	-
T8062	37221-37228	NNS	denotes	embryos
T8066	37228-37229	.	denotes	.
T8067	37229-37396	sentence	denotes	Interestingly, the lower lung/body ratios at E18.5 in Dhcr7-/- embryos compared to wild-type suggested a disproportionate inhibition of lung growth than other organs.
T8068	37230-37243	RB	denotes	Interestingly
T8070	37243-37245	,	denotes	,
T8071	37245-37248	DT	denotes	the
T8073	37249-37254	JJR	denotes	lower
T8074	37255-37259	NN	denotes	lung
T8075	37259-37260	SYM	denotes	/
T8076	37260-37264	NN	denotes	body
T8072	37265-37271	NNS	denotes	ratios
T8077	37272-37274	IN	denotes	at
T8078	37275-37280	NN	denotes	E18.5
T8079	37281-37283	IN	denotes	in
T8080	37284-37289	NN	denotes	Dhcr7
T8082	37289-37290	SYM	denotes	-
T8083	37290-37291	SYM	denotes	/
T8084	37291-37292	SYM	denotes	-
T8081	37293-37300	NNS	denotes	embryos
T8085	37301-37309	VBN	denotes	compared
T8086	37310-37312	IN	denotes	to
T8087	37313-37317	JJ	denotes	wild
T8089	37317-37318	HYPH	denotes	-
T8088	37318-37322	NN	denotes	type
T8069	37323-37332	VBD	denotes	suggested
T8090	37333-37334	DT	denotes	a
T8092	37335-37351	JJ	denotes	disproportionate
T8091	37352-37362	NN	denotes	inhibition
T8093	37363-37365	IN	denotes	of
T8094	37366-37370	NN	denotes	lung
T8095	37371-37377	NN	denotes	growth
T8096	37378-37382	IN	denotes	than
T8097	37383-37388	JJ	denotes	other
T8098	37389-37395	NNS	denotes	organs
T8099	37395-37396	.	denotes	.
T8100	37396-37514	sentence	denotes	Cell proliferation and division in Dhcr7-/- lungs were markedly arrested without apparent increase in cell apoptosis.
T8101	37397-37401	NN	denotes	Cell
T8102	37402-37415	NN	denotes	proliferation
T8104	37416-37419	CC	denotes	and
T8105	37420-37428	NN	denotes	division
T8106	37429-37431	IN	denotes	in
T8107	37432-37437	NN	denotes	Dhcr7
T8109	37437-37438	SYM	denotes	-
T8110	37438-37439	SYM	denotes	/
T8111	37439-37440	SYM	denotes	-
T8108	37441-37446	NNS	denotes	lungs
T8112	37447-37451	VBD	denotes	were
T8113	37452-37460	RB	denotes	markedly
T8103	37461-37469	VBN	denotes	arrested
T8114	37470-37477	IN	denotes	without
T8115	37478-37486	JJ	denotes	apparent
T8116	37487-37495	NN	denotes	increase
T8117	37496-37498	IN	denotes	in
T8118	37499-37503	NN	denotes	cell
T8119	37504-37513	NN	denotes	apoptosis
T8120	37513-37514	.	denotes	.
T8121	37514-37705	sentence	denotes	Therefore, it appears that growth retardation caused by cholesterol deficiency was primarily because of an inhibition of cell growth and proliferation, rather than an increase in cell death.
T8122	37515-37524	RB	denotes	Therefore
T8124	37524-37526	,	denotes	,
T8125	37526-37528	PRP	denotes	it
T8123	37529-37536	VBZ	denotes	appears
T8126	37537-37541	IN	denotes	that
T8128	37542-37548	NN	denotes	growth
T8129	37549-37560	NN	denotes	retardation
T8130	37561-37567	VBN	denotes	caused
T8131	37568-37570	IN	denotes	by
T8132	37571-37582	NN	denotes	cholesterol
T8133	37583-37593	NN	denotes	deficiency
T8127	37594-37597	VBD	denotes	was
T8134	37598-37607	RB	denotes	primarily
T8135	37608-37615	IN	denotes	because
T8136	37616-37618	IN	denotes	of
T8137	37619-37621	DT	denotes	an
T8138	37622-37632	NN	denotes	inhibition
T8139	37633-37635	IN	denotes	of
T8140	37636-37640	NN	denotes	cell
T8141	37641-37647	NN	denotes	growth
T8142	37648-37651	CC	denotes	and
T8143	37652-37665	NN	denotes	proliferation
T8144	37665-37667	,	denotes	,
T8145	37667-37673	RB	denotes	rather
T8146	37674-37678	IN	denotes	than
T8147	37679-37681	DT	denotes	an
T8148	37682-37690	NN	denotes	increase
T8149	37691-37693	IN	denotes	in
T8150	37694-37698	NN	denotes	cell
T8151	37699-37704	NN	denotes	death
T8152	37704-37705	.	denotes	.
T8153	37705-37835	sentence	denotes	Cholesterol is present in Dhcr7-/- embryos, as it is in Sc5d-/- (lathosterolosis) and Dhcr24-/-(desmosterolosis) embryos [15,38].
T8154	37706-37717	NN	denotes	Cholesterol
T8155	37718-37720	VBZ	denotes	is
T8156	37721-37728	JJ	denotes	present
T8157	37729-37731	IN	denotes	in
T8158	37732-37737	NN	denotes	Dhcr7
T8160	37737-37738	SYM	denotes	-
T8161	37738-37739	SYM	denotes	/
T8162	37739-37740	SYM	denotes	-
T8159	37741-37748	NNS	denotes	embryos
T8163	37748-37750	,	denotes	,
T8164	37750-37752	IN	denotes	as
T8166	37753-37755	PRP	denotes	it
T8165	37756-37758	VBZ	denotes	is
T8167	37759-37761	IN	denotes	in
T8168	37762-37766	NN	denotes	Sc5d
T8170	37766-37767	SYM	denotes	-
T8171	37767-37768	SYM	denotes	/
T8172	37768-37769	SYM	denotes	-
T8173	37770-37771	-LRB-	denotes	(
T8174	37771-37786	NN	denotes	lathosterolosis
T8175	37786-37787	-RRB-	denotes	)
T8176	37788-37791	CC	denotes	and
T8177	37792-37798	NN	denotes	Dhcr24
T8178	37798-37799	SYM	denotes	-
T8179	37799-37800	SYM	denotes	/
T8180	37800-37801	SYM	denotes	-
T8181	37801-37802	-LRB-	denotes	(
T8182	37802-37817	NN	denotes	desmosterolosis
T8183	37817-37818	-RRB-	denotes	)
T8169	37819-37826	NNS	denotes	embryos
T8184	37827-37828	-LRB-	denotes	[
T8186	37828-37830	CD	denotes	15
T8187	37830-37831	,	denotes	,
T8185	37831-37833	CD	denotes	38
T8188	37833-37834	-RRB-	denotes	]
T8189	37834-37835	.	denotes	.
T8190	37835-38119	sentence	denotes	Total sterols and cholesterol in Dhcr7-/- embryonic lungs (as well as in brain and liver, data not shown) were reduced and 7DHC/8DHC elevated, but a significant amount of cholesterol was detectable, comprising ~60% and ~30% of total tissue sterols at E13.5 and neonate, respectively.
T8191	37836-37841	JJ	denotes	Total
T8192	37842-37849	NNS	denotes	sterols
T8194	37850-37853	CC	denotes	and
T8195	37854-37865	NN	denotes	cholesterol
T8196	37866-37868	IN	denotes	in
T8197	37869-37874	NN	denotes	Dhcr7
T8199	37874-37875	SYM	denotes	-
T8200	37875-37876	SYM	denotes	/
T8201	37876-37877	SYM	denotes	-
T8202	37878-37887	JJ	denotes	embryonic
T8198	37888-37893	NNS	denotes	lungs
T8203	37894-37895	-LRB-	denotes	(
T8204	37895-37897	RB	denotes	as
T8206	37898-37902	RB	denotes	well
T8205	37903-37905	IN	denotes	as
T8207	37906-37908	IN	denotes	in
T8208	37909-37914	NN	denotes	brain
T8209	37915-37918	CC	denotes	and
T8210	37919-37924	NN	denotes	liver
T8211	37924-37926	,	denotes	,
T8213	37926-37930	NNS	denotes	data
T8214	37931-37934	RB	denotes	not
T8212	37935-37940	VBN	denotes	shown
T8215	37940-37941	-RRB-	denotes	)
T8216	37942-37946	VBD	denotes	were
T8193	37947-37954	VBN	denotes	reduced
T8217	37955-37958	CC	denotes	and
T8218	37959-37963	NN	denotes	7DHC
T8220	37963-37964	SYM	denotes	/
T8219	37964-37968	NN	denotes	8DHC
T8221	37969-37977	VBN	denotes	elevated
T8222	37977-37979	,	denotes	,
T8223	37979-37982	CC	denotes	but
T8224	37983-37984	DT	denotes	a
T8226	37985-37996	JJ	denotes	significant
T8225	37997-38003	NN	denotes	amount
T8228	38004-38006	IN	denotes	of
T8229	38007-38018	NN	denotes	cholesterol
T8227	38019-38022	VBD	denotes	was
T8230	38023-38033	JJ	denotes	detectable
T8231	38033-38035	,	denotes	,
T8232	38035-38045	VBG	denotes	comprising
T8233	38046-38047	SYM	denotes	~
T8234	38047-38049	CD	denotes	60
T8235	38049-38050	NN	denotes	%
T8236	38051-38054	CC	denotes	and
T8237	38055-38056	SYM	denotes	~
T8238	38056-38058	CD	denotes	30
T8239	38058-38059	NN	denotes	%
T8240	38060-38062	IN	denotes	of
T8241	38063-38068	JJ	denotes	total
T8243	38069-38075	NN	denotes	tissue
T8242	38076-38083	NNS	denotes	sterols
T8244	38084-38086	IN	denotes	at
T8245	38087-38092	NN	denotes	E13.5
T8246	38093-38096	CC	denotes	and
T8247	38097-38104	NN	denotes	neonate
T8248	38104-38106	,	denotes	,
T8249	38106-38118	RB	denotes	respectively
T8250	38118-38119	.	denotes	.
T8251	38119-38286	sentence	denotes	Fetal cholesterol can either be synthesized endogenously in fetal tissues or accrued from extra-embryonic tissues such as maternal serum, placenta, and yolk sac [39].
T8252	38120-38125	JJ	denotes	Fetal
T8253	38126-38137	NN	denotes	cholesterol
T8255	38138-38141	MD	denotes	can
T8256	38142-38148	CC	denotes	either
T8257	38149-38151	VB	denotes	be
T8254	38152-38163	VBN	denotes	synthesized
T8258	38164-38176	RB	denotes	endogenously
T8259	38177-38179	IN	denotes	in
T8260	38180-38185	JJ	denotes	fetal
T8261	38186-38193	NNS	denotes	tissues
T8262	38194-38196	CC	denotes	or
T8263	38197-38204	VBN	denotes	accrued
T8264	38205-38209	IN	denotes	from
T8265	38210-38225	JJ	denotes	extra-embryonic
T8266	38226-38233	NNS	denotes	tissues
T8267	38234-38238	JJ	denotes	such
T8268	38239-38241	IN	denotes	as
T8269	38242-38250	JJ	denotes	maternal
T8270	38251-38256	NN	denotes	serum
T8271	38256-38258	,	denotes	,
T8272	38258-38266	NN	denotes	placenta
T8273	38266-38268	,	denotes	,
T8274	38268-38271	CC	denotes	and
T8275	38272-38276	NN	denotes	yolk
T8276	38277-38280	NN	denotes	sac
T8277	38281-38282	-LRB-	denotes	[
T8278	38282-38284	CD	denotes	39
T8279	38284-38285	-RRB-	denotes	]
T8280	38285-38286	.	denotes	.
T8281	38286-38424	sentence	denotes	Since these mice theoretically can not synthesize cholesterol, any accumulation of cholesterol must be derived from the maternal sources.
T8282	38287-38292	IN	denotes	Since
T8284	38293-38298	DT	denotes	these
T8285	38299-38303	NNS	denotes	mice
T8286	38304-38317	RB	denotes	theoretically
T8287	38318-38321	MD	denotes	can
T8288	38322-38325	RB	denotes	not
T8283	38326-38336	VB	denotes	synthesize
T8290	38337-38348	NN	denotes	cholesterol
T8291	38348-38350	,	denotes	,
T8292	38350-38353	DT	denotes	any
T8293	38354-38366	NN	denotes	accumulation
T8294	38367-38369	IN	denotes	of
T8295	38370-38381	NN	denotes	cholesterol
T8296	38382-38386	MD	denotes	must
T8297	38387-38389	VB	denotes	be
T8289	38390-38397	VBN	denotes	derived
T8298	38398-38402	IN	denotes	from
T8299	38403-38406	DT	denotes	the
T8301	38407-38415	JJ	denotes	maternal
T8300	38416-38423	NNS	denotes	sources
T8302	38423-38424	.	denotes	.
T8303	38424-38672	sentence	denotes	Interestingly, Dhcr24 null mice are viable but contained almost no cholesterol in plasma and tissues at 3-months, whereas cholesterol content in Dhcr24 null embryo tissues accounts for ~60% at E11.5 and ~30% at E17.5, of total tissue sterols [38].
T8304	38425-38438	RB	denotes	Interestingly
T8306	38438-38440	,	denotes	,
T8307	38440-38446	NN	denotes	Dhcr24
T8308	38447-38451	JJ	denotes	null
T8309	38452-38456	NNS	denotes	mice
T8305	38457-38460	VBP	denotes	are
T8310	38461-38467	JJ	denotes	viable
T8311	38468-38471	CC	denotes	but
T8312	38472-38481	VBD	denotes	contained
T8313	38482-38488	RB	denotes	almost
T8315	38489-38491	DT	denotes	no
T8314	38492-38503	NN	denotes	cholesterol
T8316	38504-38506	IN	denotes	in
T8317	38507-38513	NN	denotes	plasma
T8318	38514-38517	CC	denotes	and
T8319	38518-38525	NNS	denotes	tissues
T8320	38526-38528	IN	denotes	at
T8321	38529-38530	CD	denotes	3
T8323	38530-38531	HYPH	denotes	-
T8322	38531-38537	NNS	denotes	months
T8324	38537-38539	,	denotes	,
T8325	38539-38546	IN	denotes	whereas
T8327	38547-38558	NN	denotes	cholesterol
T8328	38559-38566	NN	denotes	content
T8329	38567-38569	IN	denotes	in
T8330	38570-38576	NN	denotes	Dhcr24
T8331	38577-38581	JJ	denotes	null
T8333	38582-38588	NN	denotes	embryo
T8332	38589-38596	NNS	denotes	tissues
T8326	38597-38605	VBZ	denotes	accounts
T8334	38606-38609	IN	denotes	for
T8335	38610-38611	SYM	denotes	~
T8336	38611-38613	CD	denotes	60
T8337	38613-38614	NN	denotes	%
T8338	38615-38617	IN	denotes	at
T8339	38618-38623	NN	denotes	E11.5
T8340	38624-38627	CC	denotes	and
T8341	38628-38629	SYM	denotes	~
T8342	38629-38631	CD	denotes	30
T8343	38631-38632	NN	denotes	%
T8344	38633-38635	IN	denotes	at
T8345	38636-38641	NN	denotes	E17.5
T8346	38641-38643	,	denotes	,
T8347	38643-38645	IN	denotes	of
T8348	38646-38651	JJ	denotes	total
T8350	38652-38658	NN	denotes	tissue
T8349	38659-38666	NNS	denotes	sterols
T8351	38667-38668	-LRB-	denotes	[
T8352	38668-38670	CD	denotes	38
T8353	38670-38671	-RRB-	denotes	]
T8354	38671-38672	.	denotes	.
T8355	38672-38809	sentence	denotes	These results, as well as this study, suggest that a considerable amount of maternal cholesterol can be transferred to the murine fetus.
T8356	38673-38678	DT	denotes	These
T8357	38679-38686	NNS	denotes	results
T8359	38686-38688	,	denotes	,
T8360	38688-38690	RB	denotes	as
T8362	38691-38695	RB	denotes	well
T8361	38696-38698	IN	denotes	as
T8363	38699-38703	DT	denotes	this
T8364	38704-38709	NN	denotes	study
T8365	38709-38711	,	denotes	,
T8358	38711-38718	VBP	denotes	suggest
T8366	38719-38723	IN	denotes	that
T8368	38724-38725	DT	denotes	a
T8370	38726-38738	JJ	denotes	considerable
T8369	38739-38745	NN	denotes	amount
T8371	38746-38748	IN	denotes	of
T8372	38749-38757	JJ	denotes	maternal
T8373	38758-38769	NN	denotes	cholesterol
T8374	38770-38773	MD	denotes	can
T8375	38774-38776	VB	denotes	be
T8367	38777-38788	VBN	denotes	transferred
T8376	38789-38791	IN	denotes	to
T8377	38792-38795	DT	denotes	the
T8379	38796-38802	JJ	denotes	murine
T8378	38803-38808	NN	denotes	fetus
T8380	38808-38809	.	denotes	.
T8381	38809-39031	sentence	denotes	One explanation of why the Dhcr24-/- mice are developmentally normal but Dhcr7-/- mice are affected may be because desmosterol can functionally substitute for cholesterol, whereas the other cholesterol precursors can not.
T8382	38810-38813	CD	denotes	One
T8383	38814-38825	NN	denotes	explanation
T8385	38826-38828	IN	denotes	of
T8386	38829-38832	WRB	denotes	why
T8388	38833-38836	DT	denotes	the
T8390	38837-38843	NN	denotes	Dhcr24
T8391	38843-38844	SYM	denotes	-
T8392	38844-38845	SYM	denotes	/
T8393	38845-38846	SYM	denotes	-
T8389	38847-38851	NNS	denotes	mice
T8387	38852-38855	VBP	denotes	are
T8394	38856-38871	RB	denotes	developmentally
T8395	38872-38878	JJ	denotes	normal
T8396	38879-38882	CC	denotes	but
T8397	38883-38888	NN	denotes	Dhcr7
T8399	38888-38889	SYM	denotes	-
T8400	38889-38890	SYM	denotes	/
T8401	38890-38891	SYM	denotes	-
T8398	38892-38896	NNS	denotes	mice
T8403	38897-38900	VBP	denotes	are
T8402	38901-38909	VBN	denotes	affected
T8404	38910-38913	MD	denotes	may
T8384	38914-38916	VB	denotes	be
T8405	38917-38924	IN	denotes	because
T8407	38925-38936	NN	denotes	desmosterol
T8408	38937-38940	MD	denotes	can
T8409	38941-38953	RB	denotes	functionally
T8406	38954-38964	VB	denotes	substitute
T8410	38965-38968	IN	denotes	for
T8411	38969-38980	NN	denotes	cholesterol
T8412	38980-38982	,	denotes	,
T8413	38982-38989	IN	denotes	whereas
T8415	38990-38993	DT	denotes	the
T8417	38994-38999	JJ	denotes	other
T8418	39000-39011	NN	denotes	cholesterol
T8416	39012-39022	NNS	denotes	precursors
T8414	39023-39026	MD	denotes	can
T8419	39027-39030	RB	denotes	not
T8420	39030-39031	.	denotes	.
T8421	39031-39293	sentence	denotes	Although a mechanistic understanding of why a deficiency in cholesterol biosynthesis leads to abnormal embryonic development is lacking, a frequently advanced explanation has been that Shh signaling, involved in early developmental patterning, may be disrupted.
T8422	39032-39040	IN	denotes	Although
T8424	39041-39042	DT	denotes	a
T8426	39043-39054	JJ	denotes	mechanistic
T8425	39055-39068	NN	denotes	understanding
T8427	39069-39071	IN	denotes	of
T8428	39072-39075	WRB	denotes	why
T8430	39076-39077	DT	denotes	a
T8431	39078-39088	NN	denotes	deficiency
T8432	39089-39091	IN	denotes	in
T8433	39092-39103	NN	denotes	cholesterol
T8434	39104-39116	NN	denotes	biosynthesis
T8429	39117-39122	VBZ	denotes	leads
T8435	39123-39125	IN	denotes	to
T8436	39126-39134	JJ	denotes	abnormal
T8438	39135-39144	JJ	denotes	embryonic
T8437	39145-39156	NN	denotes	development
T8439	39157-39159	VBZ	denotes	is
T8423	39160-39167	VBG	denotes	lacking
T8441	39167-39169	,	denotes	,
T8442	39169-39170	DT	denotes	a
T8444	39171-39181	RB	denotes	frequently
T8445	39182-39190	VBN	denotes	advanced
T8443	39191-39202	NN	denotes	explanation
T8446	39203-39206	VBZ	denotes	has
T8440	39207-39211	VBN	denotes	been
T8447	39212-39216	IN	denotes	that
T8449	39217-39220	NN	denotes	Shh
T8450	39221-39230	NN	denotes	signaling
T8451	39230-39232	,	denotes	,
T8452	39232-39240	VBN	denotes	involved
T8453	39241-39243	IN	denotes	in
T8454	39244-39249	JJ	denotes	early
T8456	39250-39263	JJ	denotes	developmental
T8455	39264-39274	NN	denotes	patterning
T8457	39274-39276	,	denotes	,
T8458	39276-39279	MD	denotes	may
T8459	39280-39282	VB	denotes	be
T8448	39283-39292	VBN	denotes	disrupted
T8460	39292-39293	.	denotes	.
T8461	39293-39534	sentence	denotes	In the developing lung primordium, Shh is expressed initially at E9 and promotes branching morphogenesis, which is impaired in Shh knockout mice [24], suggesting that Shh, secreted by the epithelium, is critical for branching morphogenesis.
T8462	39294-39296	IN	denotes	In
T8464	39297-39300	DT	denotes	the
T8466	39301-39311	VBG	denotes	developing
T8467	39312-39316	NN	denotes	lung
T8465	39317-39327	NN	denotes	primordium
T8468	39327-39329	,	denotes	,
T8469	39329-39332	NN	denotes	Shh
T8470	39333-39335	VBZ	denotes	is
T8463	39336-39345	VBN	denotes	expressed
T8471	39346-39355	RB	denotes	initially
T8472	39356-39358	IN	denotes	at
T8473	39359-39361	NN	denotes	E9
T8474	39362-39365	CC	denotes	and
T8475	39366-39374	VBZ	denotes	promotes
T8476	39375-39384	VBG	denotes	branching
T8477	39385-39398	NN	denotes	morphogenesis
T8478	39398-39400	,	denotes	,
T8479	39400-39405	WDT	denotes	which
T8481	39406-39408	VBZ	denotes	is
T8480	39409-39417	VBN	denotes	impaired
T8482	39418-39420	IN	denotes	in
T8483	39421-39424	NN	denotes	Shh
T8484	39425-39433	NN	denotes	knockout
T8485	39434-39438	NNS	denotes	mice
T8486	39439-39440	-LRB-	denotes	[
T8487	39440-39442	CD	denotes	24
T8488	39442-39443	-RRB-	denotes	]
T8489	39443-39445	,	denotes	,
T8490	39445-39455	VBG	denotes	suggesting
T8491	39456-39460	IN	denotes	that
T8493	39461-39464	NN	denotes	Shh
T8494	39464-39466	,	denotes	,
T8495	39466-39474	VBN	denotes	secreted
T8496	39475-39477	IN	denotes	by
T8497	39478-39481	DT	denotes	the
T8498	39482-39492	NN	denotes	epithelium
T8499	39492-39494	,	denotes	,
T8492	39494-39496	VBZ	denotes	is
T8500	39497-39505	JJ	denotes	critical
T8501	39506-39509	IN	denotes	for
T8502	39510-39519	VBG	denotes	branching
T8503	39520-39533	NN	denotes	morphogenesis
T8504	39533-39534	.	denotes	.
T8505	39534-39822	sentence	denotes	In this study, the lack of evidence of abnormal lung branching morphogenesis in the early stages, but a consistent delayed maturation of the gas-exchange region of the lung in the saccular period of development of Dhcr7-/- embryos was observed, suggesting early Shh signaling was normal.
T8506	39535-39537	IN	denotes	In
T8508	39538-39542	DT	denotes	this
T8509	39543-39548	NN	denotes	study
T8510	39548-39550	,	denotes	,
T8511	39550-39553	DT	denotes	the
T8512	39554-39558	NN	denotes	lack
T8513	39559-39561	IN	denotes	of
T8514	39562-39570	NN	denotes	evidence
T8515	39571-39573	IN	denotes	of
T8516	39574-39582	JJ	denotes	abnormal
T8518	39583-39587	NN	denotes	lung
T8519	39588-39597	VBG	denotes	branching
T8517	39598-39611	NN	denotes	morphogenesis
T8520	39612-39614	IN	denotes	in
T8521	39615-39618	DT	denotes	the
T8523	39619-39624	JJ	denotes	early
T8522	39625-39631	NNS	denotes	stages
T8524	39631-39633	,	denotes	,
T8525	39633-39636	CC	denotes	but
T8526	39637-39638	DT	denotes	a
T8528	39639-39649	JJ	denotes	consistent
T8529	39650-39657	VBN	denotes	delayed
T8527	39658-39668	NN	denotes	maturation
T8530	39669-39671	IN	denotes	of
T8531	39672-39675	DT	denotes	the
T8533	39676-39679	NN	denotes	gas
T8535	39679-39680	HYPH	denotes	-
T8534	39680-39688	NN	denotes	exchange
T8532	39689-39695	NN	denotes	region
T8536	39696-39698	IN	denotes	of
T8537	39699-39702	DT	denotes	the
T8538	39703-39707	NN	denotes	lung
T8539	39708-39710	IN	denotes	in
T8540	39711-39714	DT	denotes	the
T8542	39715-39723	JJ	denotes	saccular
T8541	39724-39730	NN	denotes	period
T8543	39731-39733	IN	denotes	of
T8544	39734-39745	NN	denotes	development
T8545	39746-39748	IN	denotes	of
T8546	39749-39754	NN	denotes	Dhcr7
T8548	39754-39755	SYM	denotes	-
T8549	39755-39756	SYM	denotes	/
T8550	39756-39757	SYM	denotes	-
T8547	39758-39765	NNS	denotes	embryos
T8551	39766-39769	VBD	denotes	was
T8507	39770-39778	VBN	denotes	observed
T8552	39778-39780	,	denotes	,
T8553	39780-39790	VBG	denotes	suggesting
T8554	39791-39796	JJ	denotes	early
T8556	39797-39800	NN	denotes	Shh
T8555	39801-39810	NN	denotes	signaling
T8557	39811-39814	VBD	denotes	was
T8558	39815-39821	JJ	denotes	normal
T8559	39821-39822	.	denotes	.
T8560	39822-39935	sentence	denotes	Shh protein expression and autoprocessing at E11.5 was indistinguishable between wild-type and Dhcr7-/- embryos.
T8561	39823-39826	NN	denotes	Shh
T8563	39827-39834	NN	denotes	protein
T8562	39835-39845	NN	denotes	expression
T8565	39846-39849	CC	denotes	and
T8566	39850-39864	NN	denotes	autoprocessing
T8567	39865-39867	IN	denotes	at
T8568	39868-39873	NN	denotes	E11.5
T8564	39874-39877	VBD	denotes	was
T8569	39878-39895	JJ	denotes	indistinguishable
T8570	39896-39903	IN	denotes	between
T8571	39904-39908	JJ	denotes	wild
T8573	39908-39909	HYPH	denotes	-
T8572	39909-39913	NN	denotes	type
T8575	39914-39917	CC	denotes	and
T8576	39918-39923	NN	denotes	Dhcr7
T8577	39923-39924	SYM	denotes	-
T8578	39924-39925	SYM	denotes	/
T8579	39925-39926	SYM	denotes	-
T8574	39927-39934	NNS	denotes	embryos
T8580	39934-39935	.	denotes	.
T8581	39935-40064	sentence	denotes	Additionally, although these experiments were not quantitative, the abundance of Shh appeared unchanged between these genotypes.
T8582	39936-39948	RB	denotes	Additionally
T8584	39948-39950	,	denotes	,
T8585	39950-39958	IN	denotes	although
T8587	39959-39964	DT	denotes	these
T8588	39965-39976	NNS	denotes	experiments
T8586	39977-39981	VBD	denotes	were
T8589	39982-39985	RB	denotes	not
T8590	39986-39998	JJ	denotes	quantitative
T8591	39998-40000	,	denotes	,
T8592	40000-40003	DT	denotes	the
T8593	40004-40013	NN	denotes	abundance
T8594	40014-40016	IN	denotes	of
T8595	40017-40020	NN	denotes	Shh
T8583	40021-40029	VBD	denotes	appeared
T8596	40030-40039	JJ	denotes	unchanged
T8597	40040-40047	IN	denotes	between
T8598	40048-40053	DT	denotes	these
T8599	40054-40063	NNS	denotes	genotypes
T8600	40063-40064	.	denotes	.
T8601	40064-40352	sentence	denotes	Thus, it is possible that the cholesterol levels (roughly 50% of normal levels) in Dhcr7-/- embryos were enough for completion of Shh autoprocessing or 7DHC accumulated in Dhcr7-/- embryonic tissues can also participate efficiently as a sterol adduct in the Shh processing reaction [20].
T8602	40065-40069	RB	denotes	Thus
T8604	40069-40071	,	denotes	,
T8605	40071-40073	PRP	denotes	it
T8603	40074-40076	VBZ	denotes	is
T8606	40077-40085	JJ	denotes	possible
T8607	40086-40090	IN	denotes	that
T8609	40091-40094	DT	denotes	the
T8611	40095-40106	NN	denotes	cholesterol
T8610	40107-40113	NNS	denotes	levels
T8612	40114-40115	-LRB-	denotes	(
T8614	40115-40122	RB	denotes	roughly
T8615	40123-40125	CD	denotes	50
T8613	40125-40126	NN	denotes	%
T8616	40127-40129	IN	denotes	of
T8617	40130-40136	JJ	denotes	normal
T8618	40137-40143	NNS	denotes	levels
T8619	40143-40144	-RRB-	denotes	)
T8620	40145-40147	IN	denotes	in
T8621	40148-40153	NN	denotes	Dhcr7
T8623	40153-40154	SYM	denotes	-
T8624	40154-40155	SYM	denotes	/
T8625	40155-40156	SYM	denotes	-
T8622	40157-40164	NNS	denotes	embryos
T8608	40165-40169	VBD	denotes	were
T8626	40170-40176	JJ	denotes	enough
T8627	40177-40180	IN	denotes	for
T8628	40181-40191	NN	denotes	completion
T8629	40192-40194	IN	denotes	of
T8630	40195-40198	NN	denotes	Shh
T8631	40199-40213	NN	denotes	autoprocessing
T8632	40214-40216	CC	denotes	or
T8633	40217-40221	NN	denotes	7DHC
T8635	40222-40233	VBN	denotes	accumulated
T8636	40234-40236	IN	denotes	in
T8637	40237-40242	NN	denotes	Dhcr7
T8639	40242-40243	SYM	denotes	-
T8640	40243-40244	SYM	denotes	/
T8641	40244-40245	SYM	denotes	-
T8642	40246-40255	JJ	denotes	embryonic
T8638	40256-40263	NNS	denotes	tissues
T8643	40264-40267	MD	denotes	can
T8644	40268-40272	RB	denotes	also
T8634	40273-40284	VB	denotes	participate
T8645	40285-40296	RB	denotes	efficiently
T8646	40297-40299	IN	denotes	as
T8647	40300-40301	DT	denotes	a
T8649	40302-40308	NN	denotes	sterol
T8648	40309-40315	NN	denotes	adduct
T8650	40316-40318	IN	denotes	in
T8651	40319-40322	DT	denotes	the
T8653	40323-40326	NN	denotes	Shh
T8654	40327-40337	NN	denotes	processing
T8652	40338-40346	NN	denotes	reaction
T8655	40347-40348	-LRB-	denotes	[
T8656	40348-40350	CD	denotes	20
T8657	40350-40351	-RRB-	denotes	]
T8658	40351-40352	.	denotes	.
T8659	40352-40521	sentence	denotes	In this study, we also examined the Shh signaling pathway by immunohistochemistry of known key components of this pathway, such as patched, smoothened and Gli proteins.
T8660	40353-40355	IN	denotes	In
T8662	40356-40360	DT	denotes	this
T8663	40361-40366	NN	denotes	study
T8664	40366-40368	,	denotes	,
T8665	40368-40370	PRP	denotes	we
T8666	40371-40375	RB	denotes	also
T8661	40376-40384	VBD	denotes	examined
T8667	40385-40388	DT	denotes	the
T8669	40389-40392	NN	denotes	Shh
T8670	40393-40402	NN	denotes	signaling
T8668	40403-40410	NN	denotes	pathway
T8671	40411-40413	IN	denotes	by
T8672	40414-40434	NN	denotes	immunohistochemistry
T8673	40435-40437	IN	denotes	of
T8674	40438-40443	JJ	denotes	known
T8676	40444-40447	JJ	denotes	key
T8675	40448-40458	NNS	denotes	components
T8677	40459-40461	IN	denotes	of
T8678	40462-40466	DT	denotes	this
T8679	40467-40474	NN	denotes	pathway
T8680	40474-40476	,	denotes	,
T8681	40476-40480	JJ	denotes	such
T8682	40481-40483	IN	denotes	as
T8683	40484-40491	VBN	denotes	patched
T8685	40491-40493	,	denotes	,
T8686	40493-40503	VBN	denotes	smoothened
T8687	40504-40507	CC	denotes	and
T8688	40508-40511	NN	denotes	Gli
T8684	40512-40520	NN	denotes	proteins
T8689	40520-40521	.	denotes	.
T8690	40521-40634	sentence	denotes	Shh signaling leads to the activation of transcription and increased protein levels of these components [40-42].
T8691	40522-40525	NN	denotes	Shh
T8692	40526-40535	NN	denotes	signaling
T8693	40536-40541	VBZ	denotes	leads
T8694	40542-40544	IN	denotes	to
T8695	40545-40548	DT	denotes	the
T8696	40549-40559	NN	denotes	activation
T8697	40560-40562	IN	denotes	of
T8698	40563-40576	NN	denotes	transcription
T8699	40577-40580	CC	denotes	and
T8700	40581-40590	VBN	denotes	increased
T8702	40591-40598	NN	denotes	protein
T8701	40599-40605	NNS	denotes	levels
T8703	40606-40608	IN	denotes	of
T8704	40609-40614	DT	denotes	these
T8705	40615-40625	NNS	denotes	components
T8706	40626-40627	-LRB-	denotes	[
T8707	40627-40629	CD	denotes	40
T8708	40629-40630	SYM	denotes	-
T8709	40630-40632	CD	denotes	42
T8710	40632-40633	-RRB-	denotes	]
T8711	40633-40634	.	denotes	.
T8712	40634-40814	sentence	denotes	If Shh signaling were defective in Dhcr7-/- embryos, these could have resulted in abnormalities of the staining patterns of these components, but no such disturbance was detected.
T8713	40635-40637	IN	denotes	If
T8715	40638-40641	NN	denotes	Shh
T8716	40642-40651	NN	denotes	signaling
T8714	40652-40656	VBD	denotes	were
T8718	40657-40666	JJ	denotes	defective
T8719	40667-40669	IN	denotes	in
T8720	40670-40675	NN	denotes	Dhcr7
T8722	40675-40676	SYM	denotes	-
T8723	40676-40677	SYM	denotes	/
T8724	40677-40678	SYM	denotes	-
T8721	40679-40686	NNS	denotes	embryos
T8725	40686-40688	,	denotes	,
T8726	40688-40693	DT	denotes	these
T8727	40694-40699	MD	denotes	could
T8728	40700-40704	VB	denotes	have
T8717	40705-40713	VBD	denotes	resulted
T8729	40714-40716	IN	denotes	in
T8730	40717-40730	NNS	denotes	abnormalities
T8731	40731-40733	IN	denotes	of
T8732	40734-40737	DT	denotes	the
T8734	40738-40746	VBG	denotes	staining
T8733	40747-40755	NNS	denotes	patterns
T8735	40756-40758	IN	denotes	of
T8736	40759-40764	DT	denotes	these
T8737	40765-40775	NNS	denotes	components
T8738	40775-40777	,	denotes	,
T8739	40777-40780	CC	denotes	but
T8740	40781-40783	DT	denotes	no
T8742	40784-40788	JJ	denotes	such
T8741	40789-40800	NN	denotes	disturbance
T8744	40801-40804	VBD	denotes	was
T8743	40805-40813	VBN	denotes	detected
T8745	40813-40814	.	denotes	.
T8746	40814-40964	sentence	denotes	Indeed, the patterns of protein expression seemed comparable and indistinguishable from wild-type embryos at all stages of lung development examined.
T8747	40815-40821	RB	denotes	Indeed
T8749	40821-40823	,	denotes	,
T8750	40823-40826	DT	denotes	the
T8751	40827-40835	NNS	denotes	patterns
T8752	40836-40838	IN	denotes	of
T8753	40839-40846	NN	denotes	protein
T8754	40847-40857	NN	denotes	expression
T8748	40858-40864	VBD	denotes	seemed
T8755	40865-40875	JJ	denotes	comparable
T8756	40876-40879	CC	denotes	and
T8757	40880-40897	JJ	denotes	indistinguishable
T8758	40898-40902	IN	denotes	from
T8759	40903-40907	JJ	denotes	wild
T8761	40907-40908	HYPH	denotes	-
T8760	40908-40912	NN	denotes	type
T8762	40913-40920	NNS	denotes	embryos
T8763	40921-40923	IN	denotes	at
T8764	40924-40927	DT	denotes	all
T8765	40928-40934	NNS	denotes	stages
T8766	40935-40937	IN	denotes	of
T8767	40938-40942	NN	denotes	lung
T8768	40943-40954	NN	denotes	development
T8769	40955-40963	VBN	denotes	examined
T8770	40963-40964	.	denotes	.
T8771	40964-41166	sentence	denotes	In a recent in vitro study [43], mouse embryonic fibroblasts (MEFs) from Dhcr7-/- embryos, grown in lipid-depleted culture and transiently treated with cyclodextrin, showed no affect on Shh processing.
T8772	40965-40967	IN	denotes	In
T8774	40968-40969	DT	denotes	a
T8776	40970-40976	JJ	denotes	recent
T8777	40977-40979	FW	denotes	in
T8778	40980-40985	FW	denotes	vitro
T8775	40986-40991	NN	denotes	study
T8779	40992-40993	-LRB-	denotes	[
T8780	40993-40995	CD	denotes	43
T8781	40995-40996	-RRB-	denotes	]
T8782	40996-40998	,	denotes	,
T8783	40998-41003	NN	denotes	mouse
T8785	41004-41013	JJ	denotes	embryonic
T8784	41014-41025	NNS	denotes	fibroblasts
T8786	41026-41027	-LRB-	denotes	(
T8787	41027-41031	NNS	denotes	MEFs
T8788	41031-41032	-RRB-	denotes	)
T8789	41033-41037	IN	denotes	from
T8790	41038-41043	NN	denotes	Dhcr7
T8792	41043-41044	SYM	denotes	-
T8793	41044-41045	SYM	denotes	/
T8794	41045-41046	SYM	denotes	-
T8791	41047-41054	NNS	denotes	embryos
T8795	41054-41056	,	denotes	,
T8796	41056-41061	VBN	denotes	grown
T8797	41062-41064	IN	denotes	in
T8798	41065-41070	NN	denotes	lipid
T8800	41070-41071	HYPH	denotes	-
T8799	41071-41079	VBN	denotes	depleted
T8801	41080-41087	NN	denotes	culture
T8802	41088-41091	CC	denotes	and
T8803	41092-41103	RB	denotes	transiently
T8804	41104-41111	VBN	denotes	treated
T8805	41112-41116	IN	denotes	with
T8806	41117-41129	NN	denotes	cyclodextrin
T8807	41129-41131	,	denotes	,
T8773	41131-41137	VBD	denotes	showed
T8808	41138-41140	DT	denotes	no
T8809	41141-41147	NN	denotes	affect
T8810	41148-41150	IN	denotes	on
T8811	41151-41154	NN	denotes	Shh
T8812	41155-41165	NN	denotes	processing
T8813	41165-41166	.	denotes	.
T8814	41166-41292	sentence	denotes	However, a deficiency in their ability to respond to Shh activation, as judged by reporter gene expression, was demonstrable.
T8815	41167-41174	RB	denotes	However
T8817	41174-41176	,	denotes	,
T8818	41176-41177	DT	denotes	a
T8819	41178-41188	NN	denotes	deficiency
T8820	41189-41191	IN	denotes	in
T8821	41192-41197	PRP$	denotes	their
T8822	41198-41205	NN	denotes	ability
T8823	41206-41208	TO	denotes	to
T8824	41209-41216	VB	denotes	respond
T8825	41217-41219	IN	denotes	to
T8826	41220-41223	NN	denotes	Shh
T8827	41224-41234	NN	denotes	activation
T8828	41234-41236	,	denotes	,
T8829	41236-41238	IN	denotes	as
T8830	41239-41245	VBN	denotes	judged
T8831	41246-41248	IN	denotes	by
T8832	41249-41257	NN	denotes	reporter
T8834	41258-41262	NN	denotes	gene
T8833	41263-41273	NN	denotes	expression
T8835	41273-41275	,	denotes	,
T8816	41275-41278	VBD	denotes	was
T8836	41279-41291	JJ	denotes	demonstrable
T8837	41291-41292	.	denotes	.
T8838	41292-41449	sentence	denotes	In contrast, when CHO cells, that stably expressed Shh, were subjected to sterol deprivation, an arrest of Shh autoprocessing was clearly demonstrable [29].
T8839	41293-41295	IN	denotes	In
T8841	41296-41304	NN	denotes	contrast
T8842	41304-41306	,	denotes	,
T8843	41306-41310	WRB	denotes	when
T8845	41311-41314	NN	denotes	CHO
T8846	41315-41320	NNS	denotes	cells
T8847	41320-41322	,	denotes	,
T8848	41322-41326	WDT	denotes	that
T8850	41327-41333	RB	denotes	stably
T8849	41334-41343	VBN	denotes	expressed
T8851	41344-41347	NN	denotes	Shh
T8852	41347-41349	,	denotes	,
T8853	41349-41353	VBD	denotes	were
T8844	41354-41363	VBN	denotes	subjected
T8854	41364-41366	IN	denotes	to
T8855	41367-41373	NN	denotes	sterol
T8856	41374-41385	NN	denotes	deprivation
T8857	41385-41387	,	denotes	,
T8858	41387-41389	DT	denotes	an
T8859	41390-41396	NN	denotes	arrest
T8860	41397-41399	IN	denotes	of
T8861	41400-41403	NN	denotes	Shh
T8862	41404-41418	NN	denotes	autoprocessing
T8840	41419-41422	VBD	denotes	was
T8863	41423-41430	RB	denotes	clearly
T8864	41431-41443	JJ	denotes	demonstrable
T8865	41444-41445	-LRB-	denotes	[
T8866	41445-41447	CD	denotes	29
T8867	41447-41448	-RRB-	denotes	]
T8868	41448-41449	.	denotes	.
T8869	41449-41545	sentence	denotes	In the present study, we did not detect unprocessed Shh in Dhcr7-/- embryonic tissues at E11.5.
T8870	41450-41452	IN	denotes	In
T8872	41453-41456	DT	denotes	the
T8874	41457-41464	JJ	denotes	present
T8873	41465-41470	NN	denotes	study
T8875	41470-41472	,	denotes	,
T8876	41472-41474	PRP	denotes	we
T8877	41475-41478	VBD	denotes	did
T8878	41479-41482	RB	denotes	not
T8871	41483-41489	VB	denotes	detect
T8879	41490-41501	JJ	denotes	unprocessed
T8880	41502-41505	NN	denotes	Shh
T8881	41506-41508	IN	denotes	in
T8882	41509-41514	NN	denotes	Dhcr7
T8884	41514-41515	SYM	denotes	-
T8885	41515-41516	SYM	denotes	/
T8886	41516-41517	SYM	denotes	-
T8887	41518-41527	JJ	denotes	embryonic
T8883	41528-41535	NNS	denotes	tissues
T8888	41536-41538	IN	denotes	at
T8889	41539-41544	NN	denotes	E11.5
T8890	41544-41545	.	denotes	.
T8891	41545-41633	sentence	denotes	Subtle differences in Shh signal transduction can not be excluded by the present study.
T8892	41546-41552	JJ	denotes	Subtle
T8893	41553-41564	NNS	denotes	differences
T8895	41565-41567	IN	denotes	in
T8896	41568-41571	NN	denotes	Shh
T8897	41572-41578	NN	denotes	signal
T8898	41579-41591	NN	denotes	transduction
T8899	41592-41595	MD	denotes	can
T8900	41596-41599	RB	denotes	not
T8901	41600-41602	VB	denotes	be
T8894	41603-41611	VBN	denotes	excluded
T8902	41612-41614	IN	denotes	by
T8903	41615-41618	DT	denotes	the
T8905	41619-41626	JJ	denotes	present
T8904	41627-41632	NN	denotes	study
T8906	41632-41633	.	denotes	.
T8907	41633-41814	sentence	denotes	However, we favor a model whereby the loss of normal cholesterol biosynthesis is important, not so much for Shh autocleavage, as for the loss of the correct plasma membrane milieu.
T8908	41634-41641	RB	denotes	However
T8910	41641-41643	,	denotes	,
T8911	41643-41645	PRP	denotes	we
T8909	41646-41651	VBP	denotes	favor
T8912	41652-41653	DT	denotes	a
T8913	41654-41659	NN	denotes	model
T8914	41660-41667	WRB	denotes	whereby
T8916	41668-41671	DT	denotes	the
T8917	41672-41676	NN	denotes	loss
T8918	41677-41679	IN	denotes	of
T8919	41680-41686	JJ	denotes	normal
T8921	41687-41698	NN	denotes	cholesterol
T8920	41699-41711	NN	denotes	biosynthesis
T8915	41712-41714	VBZ	denotes	is
T8922	41715-41724	JJ	denotes	important
T8923	41724-41726	,	denotes	,
T8924	41726-41729	RB	denotes	not
T8926	41730-41732	RB	denotes	so
T8927	41733-41737	JJ	denotes	much
T8925	41738-41741	IN	denotes	for
T8928	41742-41745	NN	denotes	Shh
T8929	41746-41758	NN	denotes	autocleavage
T8930	41758-41760	,	denotes	,
T8931	41760-41762	IN	denotes	as
T8932	41763-41766	IN	denotes	for
T8933	41767-41770	DT	denotes	the
T8934	41771-41775	NN	denotes	loss
T8935	41776-41778	IN	denotes	of
T8936	41779-41782	DT	denotes	the
T8938	41783-41790	JJ	denotes	correct
T8939	41791-41797	NN	denotes	plasma
T8940	41798-41806	NN	denotes	membrane
T8937	41807-41813	NN	denotes	milieu
T8941	41813-41814	.	denotes	.
T8942	41814-42076	sentence	denotes	Since the loss of cholesterol content in the plasma membrane (and perhaps any membrane) is likely to alter the function of receptors more sensitive to this, loss of normal cholesterol synthesis may cause a loss of signal transduction of a host of such pathways.
T8943	41815-41820	IN	denotes	Since
T8945	41821-41824	DT	denotes	the
T8946	41825-41829	NN	denotes	loss
T8947	41830-41832	IN	denotes	of
T8948	41833-41844	NN	denotes	cholesterol
T8949	41845-41852	NN	denotes	content
T8950	41853-41855	IN	denotes	in
T8951	41856-41859	DT	denotes	the
T8953	41860-41866	NN	denotes	plasma
T8952	41867-41875	NN	denotes	membrane
T8954	41876-41877	-LRB-	denotes	(
T8955	41877-41880	CC	denotes	and
T8956	41881-41888	RB	denotes	perhaps
T8958	41889-41892	DT	denotes	any
T8957	41893-41901	NN	denotes	membrane
T8959	41901-41902	-RRB-	denotes	)
T8944	41903-41905	VBZ	denotes	is
T8961	41906-41912	RB	denotes	likely
T8962	41913-41915	TO	denotes	to
T8963	41916-41921	VB	denotes	alter
T8964	41922-41925	DT	denotes	the
T8965	41926-41934	NN	denotes	function
T8966	41935-41937	IN	denotes	of
T8967	41938-41947	NNS	denotes	receptors
T8968	41948-41952	RBR	denotes	more
T8969	41953-41962	JJ	denotes	sensitive
T8970	41963-41965	IN	denotes	to
T8971	41966-41970	DT	denotes	this
T8972	41970-41972	,	denotes	,
T8973	41972-41976	NN	denotes	loss
T8974	41977-41979	IN	denotes	of
T8975	41980-41986	JJ	denotes	normal
T8977	41987-41998	NN	denotes	cholesterol
T8976	41999-42008	NN	denotes	synthesis
T8978	42009-42012	MD	denotes	may
T8960	42013-42018	VB	denotes	cause
T8979	42019-42020	DT	denotes	a
T8980	42021-42025	NN	denotes	loss
T8981	42026-42028	IN	denotes	of
T8982	42029-42035	NN	denotes	signal
T8983	42036-42048	NN	denotes	transduction
T8984	42049-42051	IN	denotes	of
T8985	42052-42053	DT	denotes	a
T8986	42054-42058	NN	denotes	host
T8987	42059-42061	IN	denotes	of
T8988	42062-42066	JJ	denotes	such
T8989	42067-42075	NNS	denotes	pathways
T8990	42075-42076	.	denotes	.
T8991	42076-42173	sentence	denotes	Thus, this effect may not be limited to the Shh pathway and other targets may be more important.
T8992	42077-42081	RB	denotes	Thus
T8994	42081-42083	,	denotes	,
T8995	42083-42087	DT	denotes	this
T8996	42088-42094	NN	denotes	effect
T8997	42095-42098	MD	denotes	may
T8998	42099-42102	RB	denotes	not
T8999	42103-42105	VB	denotes	be
T8993	42106-42113	VBN	denotes	limited
T9000	42114-42116	IN	denotes	to
T9001	42117-42120	DT	denotes	the
T9003	42121-42124	NN	denotes	Shh
T9002	42125-42132	NN	denotes	pathway
T9004	42133-42136	CC	denotes	and
T9005	42137-42142	JJ	denotes	other
T9006	42143-42150	NNS	denotes	targets
T9008	42151-42154	MD	denotes	may
T9007	42155-42157	VB	denotes	be
T9009	42158-42162	RBR	denotes	more
T9010	42163-42172	JJ	denotes	important
T9011	42172-42173	.	denotes	.
T9012	42173-42236	sentence	denotes	This hypothesis is amenable to testing in the Dhcr7 null mice.
T9013	42174-42178	DT	denotes	This
T9014	42179-42189	NN	denotes	hypothesis
T9015	42190-42192	VBZ	denotes	is
T9016	42193-42201	JJ	denotes	amenable
T9017	42202-42204	IN	denotes	to
T9018	42205-42212	NN	denotes	testing
T9019	42213-42215	IN	denotes	in
T9020	42216-42219	DT	denotes	the
T9022	42220-42225	NN	denotes	Dhcr7
T9023	42226-42230	JJ	denotes	null
T9021	42231-42235	NNS	denotes	mice
T9024	42235-42236	.	denotes	.
T9079	42249-42260	NN	denotes	Cholesterol
T9080	42261-42271	NN	denotes	deficiency
T9082	42272-42278	VBN	denotes	caused
T9083	42279-42281	IN	denotes	by
T9084	42282-42287	NN	denotes	Dhcr7
T9085	42288-42298	NN	denotes	deficiency
T9086	42299-42301	IN	denotes	in
T9087	42302-42306	NNS	denotes	mice
T9081	42307-42315	VBD	denotes	resulted
T9088	42316-42318	IN	denotes	in
T9089	42318-42320	,	denotes	,
T9090	42320-42325	IN	denotes	along
T9092	42326-42330	IN	denotes	with
T9093	42331-42335	NN	denotes	IUGR
T9094	42335-42337	,	denotes	,
T9095	42337-42338	DT	denotes	a
T9096	42339-42347	JJ	denotes	distinct
T9097	42348-42352	NN	denotes	lung
T9098	42353-42361	JJ	denotes	saccular
T9091	42362-42372	NN	denotes	hypoplasia
T9099	42373-42377	IN	denotes	with
T9100	42378-42386	JJ	denotes	impaired
T9101	42387-42402	NN	denotes	differentiation
T9102	42403-42405	IN	denotes	of
T9103	42406-42410	NN	denotes	type
T9105	42411-42412	CD	denotes	I
T9106	42413-42416	CC	denotes	but
T9107	42417-42420	RB	denotes	not
T9108	42421-42425	NN	denotes	type
T9109	42426-42428	CD	denotes	II
T9104	42429-42433	NNS	denotes	AECs
T9110	42434-42437	CC	denotes	and
T9111	42438-42445	VBN	denotes	delayed
T9113	42446-42456	VBN	denotes	associated
T9114	42457-42465	JJ	denotes	vascular
T9112	42466-42477	NN	denotes	development
T9115	42477-42478	.	denotes	.
T9116	42478-42630	sentence	denotes	Loss of the correct amounts of cholesterol in the membranes may perturb those receptors that depend upon cholesterol for efficient signal transduction.
T9117	42479-42483	NN	denotes	Loss
T9119	42484-42486	IN	denotes	of
T9120	42487-42490	DT	denotes	the
T9122	42491-42498	JJ	denotes	correct
T9121	42499-42506	NNS	denotes	amounts
T9123	42507-42509	IN	denotes	of
T9124	42510-42521	NN	denotes	cholesterol
T9125	42522-42524	IN	denotes	in
T9126	42525-42528	DT	denotes	the
T9127	42529-42538	NNS	denotes	membranes
T9128	42539-42542	MD	denotes	may
T9118	42543-42550	VB	denotes	perturb
T9129	42551-42556	DT	denotes	those
T9130	42557-42566	NNS	denotes	receptors
T9131	42567-42571	WDT	denotes	that
T9132	42572-42578	VBP	denotes	depend
T9133	42579-42583	IN	denotes	upon
T9134	42584-42595	NN	denotes	cholesterol
T9135	42596-42599	IN	denotes	for
T9136	42600-42609	JJ	denotes	efficient
T9138	42610-42616	NN	denotes	signal
T9137	42617-42629	NN	denotes	transduction
T9139	42629-42630	.	denotes	.
T9140	42630-42902	sentence	denotes	Further investigation of which transcriptional and signal transduction pathways are more significantly affected by the loss of Dhcr7 will be critical to elucidate the mechanism of lung hypoplasia of this model, as well as the role of cholesterol in embryonic development.
T9141	42631-42638	JJ	denotes	Further
T9142	42639-42652	NN	denotes	investigation
T9144	42653-42655	IN	denotes	of
T9145	42656-42661	WDT	denotes	which
T9147	42662-42677	JJ	denotes	transcriptional
T9148	42678-42681	CC	denotes	and
T9149	42682-42688	NN	denotes	signal
T9150	42689-42701	NN	denotes	transduction
T9146	42702-42710	NNS	denotes	pathways
T9152	42711-42714	VBP	denotes	are
T9153	42715-42719	RBR	denotes	more
T9154	42720-42733	RB	denotes	significantly
T9151	42734-42742	VBN	denotes	affected
T9155	42743-42745	IN	denotes	by
T9156	42746-42749	DT	denotes	the
T9157	42750-42754	NN	denotes	loss
T9158	42755-42757	IN	denotes	of
T9159	42758-42763	NN	denotes	Dhcr7
T9160	42764-42768	MD	denotes	will
T9143	42769-42771	VB	denotes	be
T9161	42772-42780	JJ	denotes	critical
T9162	42781-42783	TO	denotes	to
T9163	42784-42793	VB	denotes	elucidate
T9164	42794-42797	DT	denotes	the
T9165	42798-42807	NN	denotes	mechanism
T9166	42808-42810	IN	denotes	of
T9167	42811-42815	NN	denotes	lung
T9168	42816-42826	NN	denotes	hypoplasia
T9169	42827-42829	IN	denotes	of
T9170	42830-42834	DT	denotes	this
T9171	42835-42840	NN	denotes	model
T9172	42840-42842	,	denotes	,
T9173	42842-42844	RB	denotes	as
T9175	42845-42849	RB	denotes	well
T9174	42850-42852	IN	denotes	as
T9176	42853-42856	DT	denotes	the
T9177	42857-42861	NN	denotes	role
T9178	42862-42864	IN	denotes	of
T9179	42865-42876	NN	denotes	cholesterol
T9180	42877-42879	IN	denotes	in
T9181	42880-42889	JJ	denotes	embryonic
T9182	42890-42901	NN	denotes	development
T9183	42901-42902	.	denotes	.
T9246	42913-42920	NNS	denotes	Animals
T9247	42920-43085	sentence	denotes	All mice were housed in an animal facility at 22°C with a 12 h light ~12 h dark cycle and all protocols were approved by the Institutional Animal Welfare Committee.
T9248	42921-42924	DT	denotes	All
T9249	42925-42929	NNS	denotes	mice
T9251	42930-42934	VBD	denotes	were
T9250	42935-42941	VBN	denotes	housed
T9252	42942-42944	IN	denotes	in
T9253	42945-42947	DT	denotes	an
T9255	42948-42954	NN	denotes	animal
T9254	42955-42963	NN	denotes	facility
T9256	42964-42966	IN	denotes	at
T9257	42967-42969	CD	denotes	22
T9258	42969-42971	NN	denotes	°C
T9259	42972-42976	IN	denotes	with
T9260	42977-42978	DT	denotes	a
T9262	42979-42981	CD	denotes	12
T9263	42982-42983	NN	denotes	h
T9264	42984-42989	JJ	denotes	light
T9265	42990-42991	HYPH	denotes	~
T9266	42991-42993	CD	denotes	12
T9267	42994-42995	NN	denotes	h
T9268	42996-43000	JJ	denotes	dark
T9261	43001-43006	NN	denotes	cycle
T9269	43007-43010	CC	denotes	and
T9270	43011-43014	DT	denotes	all
T9271	43015-43024	NNS	denotes	protocols
T9273	43025-43029	VBD	denotes	were
T9272	43030-43038	VBN	denotes	approved
T9274	43039-43041	IN	denotes	by
T9275	43042-43045	DT	denotes	the
T9277	43046-43059	NNP	denotes	Institutional
T9278	43060-43066	NNP	denotes	Animal
T9279	43067-43074	NNP	denotes	Welfare
T9276	43075-43084	NNP	denotes	Committee
T9280	43084-43085	.	denotes	.
T9281	43085-43211	sentence	denotes	Dhcr7 heterozygous animals used in this study have been backcrossed onto a C57BL/6J background for 11 successive generations.
T9282	43086-43091	NN	denotes	Dhcr7
T9283	43092-43104	JJ	denotes	heterozygous
T9284	43105-43112	NNS	denotes	animals
T9286	43113-43117	VBN	denotes	used
T9287	43118-43120	IN	denotes	in
T9288	43121-43125	DT	denotes	this
T9289	43126-43131	NN	denotes	study
T9290	43132-43136	VBP	denotes	have
T9291	43137-43141	VBN	denotes	been
T9285	43142-43153	VBN	denotes	backcrossed
T9292	43154-43158	IN	denotes	onto
T9293	43159-43160	DT	denotes	a
T9295	43161-43166	NN	denotes	C57BL
T9297	43166-43167	SYM	denotes	/
T9296	43167-43169	NN	denotes	6J
T9294	43170-43180	NN	denotes	background
T9298	43181-43184	IN	denotes	for
T9299	43185-43187	CD	denotes	11
T9301	43188-43198	JJ	denotes	successive
T9300	43199-43210	NNS	denotes	generations
T9302	43210-43211	.	denotes	.
T9303	43211-43282	sentence	denotes	The presence of vaginal plugs was considered as embryonic day (E) 0.5.
T9304	43212-43215	DT	denotes	The
T9305	43216-43224	NN	denotes	presence
T9307	43225-43227	IN	denotes	of
T9308	43228-43235	JJ	denotes	vaginal
T9309	43236-43241	NNS	denotes	plugs
T9310	43242-43245	VBD	denotes	was
T9306	43246-43256	VBN	denotes	considered
T9311	43257-43259	IN	denotes	as
T9312	43260-43269	JJ	denotes	embryonic
T9313	43270-43273	NN	denotes	day
T9314	43274-43275	-LRB-	denotes	(
T9315	43275-43276	NN	denotes	E
T9316	43276-43277	-RRB-	denotes	)
T9317	43278-43281	CD	denotes	0.5
T9318	43281-43282	.	denotes	.
T9319	43282-43377	sentence	denotes	Timed pregnant females were sacrificed, embryos dissected from the uteri and placed in 1X PBS.
T9320	43283-43288	VBN	denotes	Timed
T9322	43289-43297	JJ	denotes	pregnant
T9321	43298-43305	NNS	denotes	females
T9324	43306-43310	VBD	denotes	were
T9323	43311-43321	VBN	denotes	sacrificed
T9326	43321-43323	,	denotes	,
T9327	43323-43330	NNS	denotes	embryos
T9325	43331-43340	VBN	denotes	dissected
T9328	43341-43345	IN	denotes	from
T9329	43346-43349	DT	denotes	the
T9330	43350-43355	NNS	denotes	uteri
T9331	43356-43359	CC	denotes	and
T9332	43360-43366	VBN	denotes	placed
T9333	43367-43369	IN	denotes	in
T9334	43370-43372	NN	denotes	1X
T9335	43373-43376	NN	denotes	PBS
T9336	43376-43377	.	denotes	.
T9337	43377-43519	sentence	denotes	Crown-rump lengths of the embryos were measured by use of an electronic digital caliper and embryos weighed, after blotting off excess fluid.
T9338	43378-43383	NN	denotes	Crown
T9340	43383-43384	SYM	denotes	-
T9341	43384-43388	NN	denotes	rump
T9339	43389-43396	NNS	denotes	lengths
T9343	43397-43399	IN	denotes	of
T9344	43400-43403	DT	denotes	the
T9345	43404-43411	NNS	denotes	embryos
T9346	43412-43416	VBD	denotes	were
T9342	43417-43425	VBN	denotes	measured
T9347	43426-43428	IN	denotes	by
T9348	43429-43432	NN	denotes	use
T9349	43433-43435	IN	denotes	of
T9350	43436-43438	DT	denotes	an
T9352	43439-43449	JJ	denotes	electronic
T9353	43450-43457	JJ	denotes	digital
T9351	43458-43465	NN	denotes	caliper
T9354	43466-43469	CC	denotes	and
T9355	43470-43477	NNS	denotes	embryos
T9356	43478-43485	VBD	denotes	weighed
T9357	43485-43487	,	denotes	,
T9358	43487-43492	IN	denotes	after
T9359	43493-43501	VBG	denotes	blotting
T9360	43502-43505	RP	denotes	off
T9361	43506-43512	JJ	denotes	excess
T9362	43513-43518	NN	denotes	fluid
T9363	43518-43519	.	denotes	.
T9364	43519-43617	sentence	denotes	Each lung was removed intact from the thoracic cavity, blotted free of excess fluid, and weighed.
T9365	43520-43524	DT	denotes	Each
T9366	43525-43529	NN	denotes	lung
T9368	43530-43533	VBD	denotes	was
T9367	43534-43541	VBN	denotes	removed
T9369	43542-43548	JJ	denotes	intact
T9370	43549-43553	IN	denotes	from
T9371	43554-43557	DT	denotes	the
T9373	43558-43566	JJ	denotes	thoracic
T9372	43567-43573	NN	denotes	cavity
T9374	43573-43575	,	denotes	,
T9375	43575-43582	VBN	denotes	blotted
T9376	43583-43587	JJ	denotes	free
T9377	43588-43590	IN	denotes	of
T9378	43591-43597	JJ	denotes	excess
T9379	43598-43603	NN	denotes	fluid
T9380	43603-43605	,	denotes	,
T9381	43605-43608	CC	denotes	and
T9382	43609-43616	VBN	denotes	weighed
T9383	43616-43617	.	denotes	.
T9384	43617-43742	sentence	denotes	Mice were classified as P0 when birth was witnessed and their breathing activities visualized under a dissecting microscope.
T9385	43618-43622	NNS	denotes	Mice
T9387	43623-43627	VBD	denotes	were
T9386	43628-43638	VBN	denotes	classified
T9388	43639-43641	IN	denotes	as
T9389	43642-43644	NN	denotes	P0
T9390	43645-43649	WRB	denotes	when
T9392	43650-43655	NN	denotes	birth
T9393	43656-43659	VBD	denotes	was
T9391	43660-43669	VBN	denotes	witnessed
T9394	43670-43673	CC	denotes	and
T9395	43674-43679	PRP$	denotes	their
T9397	43680-43689	NN	denotes	breathing
T9396	43690-43700	NNS	denotes	activities
T9398	43701-43711	VBN	denotes	visualized
T9399	43712-43717	IN	denotes	under
T9400	43718-43719	DT	denotes	a
T9402	43720-43730	VBG	denotes	dissecting
T9401	43731-43741	NN	denotes	microscope
T9403	43741-43742	.	denotes	.
T9404	43742-43833	sentence	denotes	Digital images of embryos or lungs were captured using a Zeiss-1000 dissecting microscope.
T9405	43743-43750	JJ	denotes	Digital
T9406	43751-43757	NNS	denotes	images
T9408	43758-43760	IN	denotes	of
T9409	43761-43768	NNS	denotes	embryos
T9410	43769-43771	CC	denotes	or
T9411	43772-43777	NNS	denotes	lungs
T9412	43778-43782	VBD	denotes	were
T9407	43783-43791	VBN	denotes	captured
T9413	43792-43797	VBG	denotes	using
T9414	43798-43799	DT	denotes	a
T9416	43800-43805	NNP	denotes	Zeiss
T9417	43805-43806	HYPH	denotes	-
T9418	43806-43810	CD	denotes	1000
T9419	43811-43821	VBG	denotes	dissecting
T9415	43822-43832	NN	denotes	microscope
T9420	43832-43833	.	denotes	.
T9421	43833-43896	sentence	denotes	Genotyping was performed by PCR, as described previously [16].
T9422	43834-43844	NN	denotes	Genotyping
T9424	43845-43848	VBD	denotes	was
T9423	43849-43858	VBN	denotes	performed
T9425	43859-43861	IN	denotes	by
T9426	43862-43865	NN	denotes	PCR
T9427	43865-43867	,	denotes	,
T9428	43867-43869	IN	denotes	as
T9429	43870-43879	VBN	denotes	described
T9430	43880-43890	RB	denotes	previously
T9431	43891-43892	-LRB-	denotes	[
T9432	43892-43894	CD	denotes	16
T9433	43894-43895	-RRB-	denotes	]
T9434	43895-43896	.	denotes	.
T9435	43896-43988	sentence	denotes	And in selective cases, Southern blotting was performed for confirmation of Dhcr7 genotype.
T9436	43897-43900	CC	denotes	And
T9438	43901-43903	IN	denotes	in
T9439	43904-43913	JJ	denotes	selective
T9440	43914-43919	NNS	denotes	cases
T9441	43919-43921	,	denotes	,
T9442	43921-43929	NNP	denotes	Southern
T9443	43930-43938	VBG	denotes	blotting
T9444	43939-43942	VBD	denotes	was
T9437	43943-43952	VBN	denotes	performed
T9445	43953-43956	IN	denotes	for
T9446	43957-43969	NN	denotes	confirmation
T9447	43970-43972	IN	denotes	of
T9448	43973-43978	NN	denotes	Dhcr7
T9449	43979-43987	NN	denotes	genotype
T9450	43987-43988	.	denotes	.
T9469	43990-44001	JJ	denotes	Biochemical
T9470	44002-44014	NNS	denotes	measurements
T9471	44014-44135	sentence	denotes	Tissue sterol compositions were analyzed by gas chromatography-mass spectrometry (GC-MS) as described previously [2,44].
T9472	44015-44021	NN	denotes	Tissue
T9473	44022-44028	NN	denotes	sterol
T9474	44029-44041	NNS	denotes	compositions
T9476	44042-44046	VBD	denotes	were
T9475	44047-44055	VBN	denotes	analyzed
T9477	44056-44058	IN	denotes	by
T9478	44059-44062	NN	denotes	gas
T9479	44063-44077	NN	denotes	chromatography
T9480	44077-44078	HYPH	denotes	-
T9481	44078-44082	NN	denotes	mass
T9482	44083-44095	NN	denotes	spectrometry
T9483	44096-44097	-LRB-	denotes	(
T9484	44097-44099	NN	denotes	GC
T9486	44099-44100	HYPH	denotes	-
T9485	44100-44102	NN	denotes	MS
T9487	44102-44103	-RRB-	denotes	)
T9488	44104-44106	IN	denotes	as
T9489	44107-44116	VBN	denotes	described
T9490	44117-44127	RB	denotes	previously
T9491	44128-44129	-LRB-	denotes	[
T9493	44129-44130	CD	denotes	2
T9494	44130-44131	,	denotes	,
T9492	44131-44133	CD	denotes	44
T9495	44133-44134	-RRB-	denotes	]
T9496	44134-44135	.	denotes	.
T9497	44135-44332	sentence	denotes	Serum corticosterone levels were measured by radioimmunoassay (RIA) on the blood obtained from decapitated neonates by a commercial source (DRTC hormone Assay Core Lab, Vanderbilt University, TN).
T9498	44136-44141	NN	denotes	Serum
T9499	44142-44156	NN	denotes	corticosterone
T9500	44157-44163	NNS	denotes	levels
T9502	44164-44168	VBD	denotes	were
T9501	44169-44177	VBN	denotes	measured
T9503	44178-44180	IN	denotes	by
T9504	44181-44197	NN	denotes	radioimmunoassay
T9505	44198-44199	-LRB-	denotes	(
T9506	44199-44202	NN	denotes	RIA
T9507	44202-44203	-RRB-	denotes	)
T9508	44204-44206	IN	denotes	on
T9509	44207-44210	DT	denotes	the
T9510	44211-44216	NN	denotes	blood
T9511	44217-44225	VBN	denotes	obtained
T9512	44226-44230	IN	denotes	from
T9513	44231-44242	VBN	denotes	decapitated
T9514	44243-44251	NNS	denotes	neonates
T9515	44252-44254	IN	denotes	by
T9516	44255-44256	DT	denotes	a
T9518	44257-44267	JJ	denotes	commercial
T9517	44268-44274	NN	denotes	source
T9519	44275-44276	-LRB-	denotes	(
T9521	44276-44280	NN	denotes	DRTC
T9522	44281-44288	NN	denotes	hormone
T9523	44289-44294	NN	denotes	Assay
T9524	44295-44299	JJ	denotes	Core
T9520	44300-44303	NN	denotes	Lab
T9525	44303-44305	,	denotes	,
T9526	44305-44315	NNP	denotes	Vanderbilt
T9527	44316-44326	NNP	denotes	University
T9528	44326-44328	,	denotes	,
T9529	44328-44330	NNP	denotes	TN
T9530	44330-44331	-RRB-	denotes	)
T9531	44331-44332	.	denotes	.
T9618	44334-44343	NN	denotes	Histology
T9619	44344-44347	CC	denotes	and
T9620	44348-44368	NN	denotes	Immunohistochemistry
T9621	44368-44533	sentence	denotes	Thoracic parts from embryos or P0 mice were fixed in either Amsterdam's fixative (methanol:acetone:acetic acid:water, 35:35:5:25 v/v) or 4% paraformaldehyde in PBS.
T9622	44369-44377	JJ	denotes	Thoracic
T9623	44378-44383	NNS	denotes	parts
T9625	44384-44388	IN	denotes	from
T9626	44389-44396	NNS	denotes	embryos
T9627	44397-44399	CC	denotes	or
T9628	44400-44402	NN	denotes	P0
T9629	44403-44407	NNS	denotes	mice
T9630	44408-44412	VBD	denotes	were
T9624	44413-44418	VBN	denotes	fixed
T9631	44419-44421	IN	denotes	in
T9632	44422-44428	CC	denotes	either
T9634	44429-44438	NNP	denotes	Amsterdam
T9635	44438-44440	POS	denotes	's
T9633	44441-44449	NN	denotes	fixative
T9636	44450-44451	-LRB-	denotes	(
T9638	44451-44459	NN	denotes	methanol
T9639	44459-44460	SYM	denotes	:
T9640	44460-44467	NN	denotes	acetone
T9641	44467-44468	SYM	denotes	:
T9643	44468-44474	JJ	denotes	acetic
T9642	44475-44479	NN	denotes	acid
T9644	44479-44480	SYM	denotes	:
T9645	44480-44485	NN	denotes	water
T9646	44485-44487	,	denotes	,
T9637	44487-44489	CD	denotes	35
T9647	44489-44490	SYM	denotes	:
T9648	44490-44492	CD	denotes	35
T9649	44492-44493	SYM	denotes	:
T9650	44493-44494	CD	denotes	5
T9651	44494-44495	SYM	denotes	:
T9652	44495-44497	CD	denotes	25
T9653	44498-44499	NN	denotes	v
T9654	44499-44500	SYM	denotes	/
T9655	44500-44501	NN	denotes	v
T9656	44501-44502	-RRB-	denotes	)
T9657	44503-44505	CC	denotes	or
T9658	44506-44507	CD	denotes	4
T9659	44507-44508	NN	denotes	%
T9660	44509-44525	NN	denotes	paraformaldehyde
T9661	44526-44528	IN	denotes	in
T9662	44529-44532	NN	denotes	PBS
T9663	44532-44533	.	denotes	.
T9664	44533-44687	sentence	denotes	Tissues were fixed overnight at room temperature (RT), rinsed in PBS, dehydrated in a graded ethanol series, cleared in toluene and embedded in paraffin.
T9665	44534-44541	NNS	denotes	Tissues
T9667	44542-44546	VBD	denotes	were
T9666	44547-44552	VBN	denotes	fixed
T9668	44553-44562	RB	denotes	overnight
T9669	44563-44565	IN	denotes	at
T9670	44566-44570	NN	denotes	room
T9671	44571-44582	NN	denotes	temperature
T9672	44583-44584	-LRB-	denotes	(
T9673	44584-44586	NN	denotes	RT
T9674	44586-44587	-RRB-	denotes	)
T9675	44587-44589	,	denotes	,
T9676	44589-44595	VBN	denotes	rinsed
T9677	44596-44598	IN	denotes	in
T9678	44599-44602	NN	denotes	PBS
T9679	44602-44604	,	denotes	,
T9680	44604-44614	VBN	denotes	dehydrated
T9681	44615-44617	IN	denotes	in
T9682	44618-44619	DT	denotes	a
T9684	44620-44626	JJ	denotes	graded
T9685	44627-44634	NN	denotes	ethanol
T9683	44635-44641	NN	denotes	series
T9686	44641-44643	,	denotes	,
T9687	44643-44650	VBN	denotes	cleared
T9688	44651-44653	IN	denotes	in
T9689	44654-44661	NN	denotes	toluene
T9690	44662-44665	CC	denotes	and
T9691	44666-44674	VBN	denotes	embedded
T9692	44675-44677	IN	denotes	in
T9693	44678-44686	NN	denotes	paraffin
T9694	44686-44687	.	denotes	.
T9695	44687-44825	sentence	denotes	Five μ m sections were mounted on Superfrost-Plus slides (VWR, West Chester, PA) for histological or immunohistochemistry (IHC) analyses.
T9696	44688-44692	CD	denotes	Five
T9698	44693-44694	NN	denotes	μ
T9697	44695-44696	NN	denotes	m
T9699	44697-44705	NNS	denotes	sections
T9701	44706-44710	VBD	denotes	were
T9700	44711-44718	VBN	denotes	mounted
T9702	44719-44721	IN	denotes	on
T9703	44722-44732	NNP	denotes	Superfrost
T9705	44732-44733	HYPH	denotes	-
T9704	44733-44737	NNP	denotes	Plus
T9706	44738-44744	NNS	denotes	slides
T9707	44745-44746	-LRB-	denotes	(
T9708	44746-44749	NNP	denotes	VWR
T9709	44749-44751	,	denotes	,
T9710	44751-44755	NNP	denotes	West
T9711	44756-44763	NNP	denotes	Chester
T9712	44763-44765	,	denotes	,
T9713	44765-44767	NNP	denotes	PA
T9714	44767-44768	-RRB-	denotes	)
T9715	44769-44772	IN	denotes	for
T9716	44773-44785	JJ	denotes	histological
T9718	44786-44788	CC	denotes	or
T9719	44789-44809	NN	denotes	immunohistochemistry
T9720	44810-44811	-LRB-	denotes	(
T9721	44811-44814	NN	denotes	IHC
T9722	44814-44815	-RRB-	denotes	)
T9717	44816-44824	NNS	denotes	analyses
T9723	44824-44825	.	denotes	.
T9724	44825-44869	sentence	denotes	IHC was performed using an indirect method.
T9725	44826-44829	NN	denotes	IHC
T9727	44830-44833	VBD	denotes	was
T9726	44834-44843	VBN	denotes	performed
T9728	44844-44849	VBG	denotes	using
T9729	44850-44852	DT	denotes	an
T9731	44853-44861	JJ	denotes	indirect
T9730	44862-44868	NN	denotes	method
T9732	44868-44869	.	denotes	.
T9733	44869-45082	sentence	denotes	After deparaffination in xylene and rehydration through a graded series of ethanol, sections were heated at 95°C in 10 mM sodium citrate (pH 6.0) for an initial 15 min and for three successive 5-min periods [25].
T9734	44870-44875	IN	denotes	After
T9736	44876-44891	NN	denotes	deparaffination
T9737	44892-44894	IN	denotes	in
T9738	44895-44901	NN	denotes	xylene
T9739	44902-44905	CC	denotes	and
T9740	44906-44917	NN	denotes	rehydration
T9741	44918-44925	IN	denotes	through
T9742	44926-44927	DT	denotes	a
T9744	44928-44934	JJ	denotes	graded
T9743	44935-44941	NN	denotes	series
T9745	44942-44944	IN	denotes	of
T9746	44945-44952	NN	denotes	ethanol
T9747	44952-44954	,	denotes	,
T9748	44954-44962	NNS	denotes	sections
T9749	44963-44967	VBD	denotes	were
T9735	44968-44974	VBN	denotes	heated
T9750	44975-44977	IN	denotes	at
T9751	44978-44980	CD	denotes	95
T9752	44980-44982	NN	denotes	°C
T9753	44983-44985	IN	denotes	in
T9754	44986-44988	CD	denotes	10
T9755	44989-44991	NN	denotes	mM
T9757	44992-44998	NN	denotes	sodium
T9756	44999-45006	NN	denotes	citrate
T9758	45007-45008	-LRB-	denotes	(
T9759	45008-45010	NN	denotes	pH
T9760	45011-45014	CD	denotes	6.0
T9761	45014-45015	-RRB-	denotes	)
T9762	45016-45019	IN	denotes	for
T9763	45020-45022	DT	denotes	an
T9765	45023-45030	JJ	denotes	initial
T9766	45031-45033	CD	denotes	15
T9764	45034-45037	NN	denotes	min
T9767	45038-45041	CC	denotes	and
T9768	45042-45045	IN	denotes	for
T9769	45046-45051	CD	denotes	three
T9771	45052-45062	JJ	denotes	successive
T9772	45063-45064	CD	denotes	5
T9774	45064-45065	HYPH	denotes	-
T9773	45065-45068	NN	denotes	min
T9770	45069-45076	NNS	denotes	periods
T9775	45077-45078	-LRB-	denotes	[
T9776	45078-45080	CD	denotes	25
T9777	45080-45081	-RRB-	denotes	]
T9778	45081-45082	.	denotes	.
T9779	45082-45203	sentence	denotes	After cooling, the sections were treated with hydrogen peroxide (3% v/v in PBS, pH7.4) for 10 min and rinsed in the PBS.
T9780	45083-45088	IN	denotes	After
T9782	45089-45096	VBG	denotes	cooling
T9783	45096-45098	,	denotes	,
T9784	45098-45101	DT	denotes	the
T9785	45102-45110	NNS	denotes	sections
T9786	45111-45115	VBD	denotes	were
T9781	45116-45123	VBN	denotes	treated
T9787	45124-45128	IN	denotes	with
T9788	45129-45137	NN	denotes	hydrogen
T9789	45138-45146	NN	denotes	peroxide
T9790	45147-45148	-LRB-	denotes	(
T9792	45148-45149	CD	denotes	3
T9793	45149-45150	NN	denotes	%
T9791	45151-45152	NN	denotes	v
T9794	45152-45153	SYM	denotes	/
T9795	45153-45154	NN	denotes	v
T9796	45155-45157	IN	denotes	in
T9797	45158-45161	NN	denotes	PBS
T9798	45161-45163	,	denotes	,
T9799	45163-45168	NN	denotes	pH7.4
T9800	45168-45169	-RRB-	denotes	)
T9801	45170-45173	IN	denotes	for
T9802	45174-45176	CD	denotes	10
T9803	45177-45180	NN	denotes	min
T9804	45181-45184	CC	denotes	and
T9805	45185-45191	VBD	denotes	rinsed
T9806	45192-45194	IN	denotes	in
T9807	45195-45198	DT	denotes	the
T9808	45199-45202	NN	denotes	PBS
T9809	45202-45203	.	denotes	.
T9810	45203-45668	sentence	denotes	Sections were blocked with 5% normal serum for 2 h, washed in PBS and incubated with antibodies against Shh-N19 (1:100), Patched (1:100), Smo-N19 (1:100), Gli1 (1:100), Gli3-N19 (1:100), SP-C C19 (1:200), caveolin 1α-N20 (1:200), aquaporin 5 (AQP5, 1:200), megalin (1:200), PECAM-1 (1:200) (Santa Cruz Biotechnology, Santa Cruz, CA) or T1α (mAB 8.1.1, 1:1000 dilution, Developmental Studies Hybridoma Bank, Iowa City, IA) overnight at 4° C in a humidified chamber.
T9811	45204-45212	NNS	denotes	Sections
T9813	45213-45217	VBD	denotes	were
T9812	45218-45225	VBN	denotes	blocked
T9814	45226-45230	IN	denotes	with
T9815	45231-45232	CD	denotes	5
T9816	45232-45233	NN	denotes	%
T9818	45234-45240	JJ	denotes	normal
T9817	45241-45246	NN	denotes	serum
T9819	45247-45250	IN	denotes	for
T9820	45251-45252	CD	denotes	2
T9821	45253-45254	NN	denotes	h
T9822	45254-45256	,	denotes	,
T9823	45256-45262	VBN	denotes	washed
T9824	45263-45265	IN	denotes	in
T9825	45266-45269	NN	denotes	PBS
T9826	45270-45273	CC	denotes	and
T9827	45274-45283	VBN	denotes	incubated
T9828	45284-45288	IN	denotes	with
T9829	45289-45299	NNS	denotes	antibodies
T9830	45300-45307	IN	denotes	against
T9831	45308-45311	NN	denotes	Shh
T9833	45311-45312	HYPH	denotes	-
T9832	45312-45315	NN	denotes	N19
T9834	45316-45317	-LRB-	denotes	(
T9835	45317-45318	CD	denotes	1
T9836	45318-45319	SYM	denotes	:
T9837	45319-45322	CD	denotes	100
T9838	45322-45323	-RRB-	denotes	)
T9839	45323-45325	,	denotes	,
T9840	45325-45332	NN	denotes	Patched
T9841	45333-45334	-LRB-	denotes	(
T9842	45334-45335	CD	denotes	1
T9843	45335-45336	SYM	denotes	:
T9844	45336-45339	CD	denotes	100
T9845	45339-45340	-RRB-	denotes	)
T9846	45340-45342	,	denotes	,
T9847	45342-45345	NN	denotes	Smo
T9849	45345-45346	HYPH	denotes	-
T9848	45346-45349	NN	denotes	N19
T9850	45350-45351	-LRB-	denotes	(
T9851	45351-45352	CD	denotes	1
T9852	45352-45353	SYM	denotes	:
T9853	45353-45356	CD	denotes	100
T9854	45356-45357	-RRB-	denotes	)
T9855	45357-45359	,	denotes	,
T9856	45359-45363	NN	denotes	Gli1
T9857	45364-45365	-LRB-	denotes	(
T9858	45365-45366	CD	denotes	1
T9859	45366-45367	SYM	denotes	:
T9860	45367-45370	CD	denotes	100
T9861	45370-45371	-RRB-	denotes	)
T9862	45371-45373	,	denotes	,
T9863	45373-45377	NN	denotes	Gli3
T9865	45377-45378	HYPH	denotes	-
T9864	45378-45381	NN	denotes	N19
T9866	45382-45383	-LRB-	denotes	(
T9867	45383-45384	CD	denotes	1
T9868	45384-45385	SYM	denotes	:
T9869	45385-45388	CD	denotes	100
T9870	45388-45389	-RRB-	denotes	)
T9871	45389-45391	,	denotes	,
T9872	45391-45393	NN	denotes	SP
T9874	45393-45394	HYPH	denotes	-
T9873	45394-45395	NN	denotes	C
T9875	45396-45399	NN	denotes	C19
T9876	45400-45401	-LRB-	denotes	(
T9877	45401-45402	CD	denotes	1
T9878	45402-45403	SYM	denotes	:
T9879	45403-45406	CD	denotes	200
T9880	45406-45407	-RRB-	denotes	)
T9881	45407-45409	,	denotes	,
T9882	45409-45417	NN	denotes	caveolin
T9884	45418-45419	CD	denotes	1
T9885	45419-45420	NN	denotes	α
T9886	45420-45421	HYPH	denotes	-
T9883	45421-45424	NN	denotes	N20
T9887	45425-45426	-LRB-	denotes	(
T9888	45426-45427	CD	denotes	1
T9889	45427-45428	SYM	denotes	:
T9890	45428-45431	CD	denotes	200
T9891	45431-45432	-RRB-	denotes	)
T9892	45432-45434	,	denotes	,
T9893	45434-45443	NN	denotes	aquaporin
T9894	45444-45445	CD	denotes	5
T9895	45446-45447	-LRB-	denotes	(
T9896	45447-45451	NN	denotes	AQP5
T9897	45451-45453	,	denotes	,
T9898	45453-45454	CD	denotes	1
T9899	45454-45455	SYM	denotes	:
T9900	45455-45458	CD	denotes	200
T9901	45458-45459	-RRB-	denotes	)
T9902	45459-45461	,	denotes	,
T9903	45461-45468	NN	denotes	megalin
T9904	45469-45470	-LRB-	denotes	(
T9905	45470-45471	CD	denotes	1
T9906	45471-45472	SYM	denotes	:
T9907	45472-45475	CD	denotes	200
T9908	45475-45476	-RRB-	denotes	)
T9909	45476-45478	,	denotes	,
T9910	45478-45483	NN	denotes	PECAM
T9911	45483-45484	HYPH	denotes	-
T9912	45484-45485	CD	denotes	1
T9913	45486-45487	-LRB-	denotes	(
T9914	45487-45488	CD	denotes	1
T9915	45488-45489	SYM	denotes	:
T9916	45489-45492	CD	denotes	200
T9917	45492-45493	-RRB-	denotes	)
T9918	45494-45495	-LRB-	denotes	(
T9920	45495-45500	NNP	denotes	Santa
T9921	45501-45505	NNP	denotes	Cruz
T9919	45506-45519	NNP	denotes	Biotechnology
T9922	45519-45521	,	denotes	,
T9923	45521-45526	NNP	denotes	Santa
T9924	45527-45531	NNP	denotes	Cruz
T9925	45531-45533	,	denotes	,
T9926	45533-45535	NNP	denotes	CA
T9927	45535-45536	-RRB-	denotes	)
T9928	45537-45539	CC	denotes	or
T9929	45540-45543	NN	denotes	T1α
T9930	45544-45545	-LRB-	denotes	(
T9932	45545-45548	NN	denotes	mAB
T9933	45549-45554	CD	denotes	8.1.1
T9934	45554-45556	,	denotes	,
T9935	45556-45557	CD	denotes	1
T9937	45557-45558	SYM	denotes	:
T9938	45558-45562	CD	denotes	1000
T9936	45563-45571	NN	denotes	dilution
T9939	45571-45573	,	denotes	,
T9940	45573-45586	NNP	denotes	Developmental
T9941	45587-45594	NNPS	denotes	Studies
T9942	45595-45604	NNP	denotes	Hybridoma
T9931	45605-45609	NNP	denotes	Bank
T9943	45609-45611	,	denotes	,
T9944	45611-45615	NNP	denotes	Iowa
T9945	45616-45620	NNP	denotes	City
T9946	45620-45622	,	denotes	,
T9947	45622-45624	NNP	denotes	IA
T9948	45624-45625	-RRB-	denotes	)
T9949	45626-45635	RB	denotes	overnight
T9950	45636-45638	IN	denotes	at
T9951	45639-45640	CD	denotes	4
T9953	45640-45641	NN	denotes	°
T9952	45642-45643	NN	denotes	C
T9954	45644-45646	IN	denotes	in
T9955	45647-45648	DT	denotes	a
T9957	45649-45659	VBN	denotes	humidified
T9956	45660-45667	NN	denotes	chamber
T9958	45667-45668	.	denotes	.
T9959	45668-45979	sentence	denotes	The sections were washed in PBS, incubated with biotinylated secondary antibodies (1:1000~1:5000, Santa Cruz Biotechnology) in PBS for 2 h at RT, incubated with HRP-streptavidin complex for 30 min, rinsed in PBS and visualized using a Metal Enhanced Diaminobenzidine (DAB) Substrate Kit (Pierce, Rockford, IL).
T9960	45669-45672	DT	denotes	The
T9961	45673-45681	NNS	denotes	sections
T9963	45682-45686	VBD	denotes	were
T9962	45687-45693	VBN	denotes	washed
T9964	45694-45696	IN	denotes	in
T9965	45697-45700	NN	denotes	PBS
T9966	45700-45702	,	denotes	,
T9967	45702-45711	VBN	denotes	incubated
T9968	45712-45716	IN	denotes	with
T9969	45717-45729	VBN	denotes	biotinylated
T9971	45730-45739	JJ	denotes	secondary
T9970	45740-45750	NNS	denotes	antibodies
T9972	45751-45752	-LRB-	denotes	(
T9973	45752-45753	CD	denotes	1
T9974	45753-45754	SYM	denotes	:
T9975	45754-45758	CD	denotes	1000
T9976	45758-45759	SYM	denotes	~
T9977	45759-45760	CD	denotes	1
T9978	45760-45761	SYM	denotes	:
T9979	45761-45765	CD	denotes	5000
T9980	45765-45767	,	denotes	,
T9981	45767-45772	NNP	denotes	Santa
T9982	45773-45777	NNP	denotes	Cruz
T9983	45778-45791	NNP	denotes	Biotechnology
T9984	45791-45792	-RRB-	denotes	)
T9985	45793-45795	IN	denotes	in
T9986	45796-45799	NN	denotes	PBS
T9987	45800-45803	IN	denotes	for
T9988	45804-45805	CD	denotes	2
T9989	45806-45807	NN	denotes	h
T9990	45808-45810	IN	denotes	at
T9991	45811-45813	NN	denotes	RT
T9992	45813-45815	,	denotes	,
T9993	45815-45824	VBN	denotes	incubated
T9994	45825-45829	IN	denotes	with
T9995	45830-45833	NN	denotes	HRP
T9997	45833-45834	HYPH	denotes	-
T9996	45834-45846	NN	denotes	streptavidin
T9998	45847-45854	NN	denotes	complex
T9999	45855-45858	IN	denotes	for
T10000	45859-45861	CD	denotes	30
T10001	45862-45865	NN	denotes	min
T10002	45865-45867	,	denotes	,
T10003	45867-45873	VBN	denotes	rinsed
T10004	45874-45876	IN	denotes	in
T10005	45877-45880	NN	denotes	PBS
T10006	45881-45884	CC	denotes	and
T10007	45885-45895	VBN	denotes	visualized
T10008	45896-45901	VBG	denotes	using
T10009	45902-45903	DT	denotes	a
T10011	45904-45909	NN	denotes	Metal
T10012	45910-45918	VBN	denotes	Enhanced
T10013	45919-45935	NN	denotes	Diaminobenzidine
T10014	45936-45937	-LRB-	denotes	(
T10015	45937-45940	NN	denotes	DAB
T10016	45940-45941	-RRB-	denotes	)
T10017	45942-45951	NN	denotes	Substrate
T10010	45952-45955	NN	denotes	Kit
T10018	45956-45957	-LRB-	denotes	(
T10019	45957-45963	NNP	denotes	Pierce
T10020	45963-45965	,	denotes	,
T10021	45965-45973	NNP	denotes	Rockford
T10022	45973-45975	,	denotes	,
T10023	45975-45977	NNP	denotes	IL
T10024	45977-45978	-RRB-	denotes	)
T10025	45978-45979	.	denotes	.
T10026	45979-46091	sentence	denotes	Digital images were collected using a Polaroid Digital Microscope Camera mounted on an Olympus BX40 microscope.
T10027	45980-45987	JJ	denotes	Digital
T10028	45988-45994	NNS	denotes	images
T10030	45995-45999	VBD	denotes	were
T10029	46000-46009	VBN	denotes	collected
T10031	46010-46015	VBG	denotes	using
T10032	46016-46017	DT	denotes	a
T10034	46018-46026	NNP	denotes	Polaroid
T10035	46027-46034	NNP	denotes	Digital
T10036	46035-46045	NNP	denotes	Microscope
T10033	46046-46052	NNP	denotes	Camera
T10037	46053-46060	VBN	denotes	mounted
T10038	46061-46063	IN	denotes	on
T10039	46064-46066	DT	denotes	an
T10041	46067-46074	NNP	denotes	Olympus
T10042	46075-46079	NN	denotes	BX40
T10040	46080-46090	NN	denotes	microscope
T10043	46090-46091	.	denotes	.
T10060	46093-46097	NN	denotes	Lung
T10061	46098-46109	NN	denotes	morphometry
T10062	46109-46228	sentence	denotes	Terminal saccular space areas were measured using NIH Image software (NIH, Bethesda, MD) as previously described [45].
T10063	46110-46118	JJ	denotes	Terminal
T10065	46119-46127	JJ	denotes	saccular
T10064	46128-46133	NN	denotes	space
T10066	46134-46139	NNS	denotes	areas
T10068	46140-46144	VBD	denotes	were
T10067	46145-46153	VBN	denotes	measured
T10069	46154-46159	VBG	denotes	using
T10070	46160-46163	NNP	denotes	NIH
T10072	46164-46169	NN	denotes	Image
T10071	46170-46178	NN	denotes	software
T10073	46179-46180	-LRB-	denotes	(
T10074	46180-46183	NNP	denotes	NIH
T10075	46183-46185	,	denotes	,
T10076	46185-46193	NNP	denotes	Bethesda
T10077	46193-46195	,	denotes	,
T10078	46195-46197	NNP	denotes	MD
T10079	46197-46198	-RRB-	denotes	)
T10080	46199-46201	IN	denotes	as
T10082	46202-46212	RB	denotes	previously
T10081	46213-46222	VBN	denotes	described
T10083	46223-46224	-LRB-	denotes	[
T10084	46224-46226	CD	denotes	45
T10085	46226-46227	-RRB-	denotes	]
T10086	46227-46228	.	denotes	.
T10087	46228-46351	sentence	denotes	Multiple measurements were performed on randomly selected 0.04-mm2 fields located in the distal part of the lung sections.
T10088	46229-46237	JJ	denotes	Multiple
T10089	46238-46250	NNS	denotes	measurements
T10091	46251-46255	VBD	denotes	were
T10090	46256-46265	VBN	denotes	performed
T10092	46266-46268	IN	denotes	on
T10093	46269-46277	RB	denotes	randomly
T10094	46278-46286	VBN	denotes	selected
T10096	46287-46291	CD	denotes	0.04
T10098	46291-46292	HYPH	denotes	-
T10097	46292-46295	NN	denotes	mm2
T10095	46296-46302	NNS	denotes	fields
T10099	46303-46310	VBN	denotes	located
T10100	46311-46313	IN	denotes	in
T10101	46314-46317	DT	denotes	the
T10103	46318-46324	JJ	denotes	distal
T10102	46325-46329	NN	denotes	part
T10104	46330-46332	IN	denotes	of
T10105	46333-46336	DT	denotes	the
T10107	46337-46341	NN	denotes	lung
T10106	46342-46350	NNS	denotes	sections
T10108	46350-46351	.	denotes	.
T10109	46351-46489	sentence	denotes	The proportion of lung comprising terminal saccular spaces was calculated as a percentage of the total examined area of the lung section.
T10110	46352-46355	DT	denotes	The
T10111	46356-46366	NN	denotes	proportion
T10113	46367-46369	IN	denotes	of
T10114	46370-46374	NN	denotes	lung
T10115	46375-46385	VBG	denotes	comprising
T10116	46386-46394	JJ	denotes	terminal
T10118	46395-46403	JJ	denotes	saccular
T10117	46404-46410	NNS	denotes	spaces
T10119	46411-46414	VBD	denotes	was
T10112	46415-46425	VBN	denotes	calculated
T10120	46426-46428	IN	denotes	as
T10121	46429-46430	DT	denotes	a
T10122	46431-46441	NN	denotes	percentage
T10123	46442-46444	IN	denotes	of
T10124	46445-46448	DT	denotes	the
T10126	46449-46454	JJ	denotes	total
T10127	46455-46463	VBN	denotes	examined
T10125	46464-46468	NN	denotes	area
T10128	46469-46471	IN	denotes	of
T10129	46472-46475	DT	denotes	the
T10131	46476-46480	NN	denotes	lung
T10130	46481-46488	NN	denotes	section
T10132	46488-46489	.	denotes	.
T10133	46489-46531	sentence	denotes	Three animals per genotype were examined.
T10134	46490-46495	CD	denotes	Three
T10135	46496-46503	NNS	denotes	animals
T10137	46504-46507	IN	denotes	per
T10138	46508-46516	NN	denotes	genotype
T10139	46517-46521	VBD	denotes	were
T10136	46522-46530	VBN	denotes	examined
T10140	46530-46531	.	denotes	.
T10257	46533-46543	NN	denotes	Assessment
T10258	46544-46546	IN	denotes	of
T10259	46547-46551	NN	denotes	cell
T10261	46552-46565	NN	denotes	proliferation
T10262	46565-46566	SYM	denotes	/
T10263	46566-46574	NN	denotes	Division
T10264	46574-46575	SYM	denotes	/
T10260	46575-46580	NN	denotes	death
T10265	46580-46768	sentence	denotes	Changes in cell proliferation and division were assessed by proliferating cell nuclear antigen (PCNA) IHC, bromodeoxyuridine (BrdU) incorporation and phosphorylated histone H3 (pHH3) IHC.
T10266	46581-46588	NNS	denotes	Changes
T10268	46589-46591	IN	denotes	in
T10269	46592-46596	NN	denotes	cell
T10270	46597-46610	NN	denotes	proliferation
T10271	46611-46614	CC	denotes	and
T10272	46615-46623	NN	denotes	division
T10273	46624-46628	VBD	denotes	were
T10267	46629-46637	VBN	denotes	assessed
T10274	46638-46640	IN	denotes	by
T10275	46641-46654	VBG	denotes	proliferating
T10277	46655-46659	NN	denotes	cell
T10278	46660-46667	JJ	denotes	nuclear
T10276	46668-46675	NN	denotes	antigen
T10280	46676-46677	-LRB-	denotes	(
T10281	46677-46681	NN	denotes	PCNA
T10282	46681-46682	-RRB-	denotes	)
T10279	46683-46686	NN	denotes	IHC
T10283	46686-46688	,	denotes	,
T10284	46688-46705	NN	denotes	bromodeoxyuridine
T10286	46706-46707	-LRB-	denotes	(
T10287	46707-46711	NN	denotes	BrdU
T10288	46711-46712	-RRB-	denotes	)
T10285	46713-46726	NN	denotes	incorporation
T10289	46727-46730	CC	denotes	and
T10290	46731-46745	VBN	denotes	phosphorylated
T10292	46746-46753	NN	denotes	histone
T10291	46754-46756	NN	denotes	H3
T10294	46757-46758	-LRB-	denotes	(
T10295	46758-46762	NN	denotes	pHH3
T10296	46762-46763	-RRB-	denotes	)
T10293	46764-46767	NN	denotes	IHC
T10297	46767-46768	.	denotes	.
T10298	46768-46865	sentence	denotes	The PCNA (1:2000) and pHH3-Ser28 (1:200) antibodies were obtained from Santa Cruz Biotechnology.
T10299	46769-46772	DT	denotes	The
T10300	46773-46777	NN	denotes	PCNA
T10302	46778-46779	-LRB-	denotes	(
T10303	46779-46780	CD	denotes	1
T10304	46780-46781	SYM	denotes	:
T10305	46781-46785	CD	denotes	2000
T10306	46785-46786	-RRB-	denotes	)
T10307	46787-46790	CC	denotes	and
T10308	46791-46795	NN	denotes	pHH3
T10310	46795-46796	HYPH	denotes	-
T10309	46796-46801	NN	denotes	Ser28
T10311	46802-46803	-LRB-	denotes	(
T10312	46803-46804	CD	denotes	1
T10313	46804-46805	SYM	denotes	:
T10314	46805-46808	CD	denotes	200
T10315	46808-46809	-RRB-	denotes	)
T10301	46810-46820	NNS	denotes	antibodies
T10317	46821-46825	VBD	denotes	were
T10316	46826-46834	VBN	denotes	obtained
T10318	46835-46839	IN	denotes	from
T10319	46840-46845	NNP	denotes	Santa
T10320	46846-46850	NNP	denotes	Cruz
T10321	46851-46864	NNP	denotes	Biotechnology
T10322	46864-46865	.	denotes	.
T10323	46865-47095	sentence	denotes	For BrdU incorporation, timed pregnant mice at E18.5 were injected intra-peritoneally with 100 μg/gm body weight of BrdU (Sigma, St Louis, MO) 2 h prior to sacrifice, embryos dissected from uteri and processed as described above.
T10324	46866-46869	IN	denotes	For
T10326	46870-46874	NN	denotes	BrdU
T10327	46875-46888	NN	denotes	incorporation
T10328	46888-46890	,	denotes	,
T10329	46890-46895	VBN	denotes	timed
T10331	46896-46904	JJ	denotes	pregnant
T10330	46905-46909	NNS	denotes	mice
T10332	46910-46912	IN	denotes	at
T10333	46913-46918	NN	denotes	E18.5
T10334	46919-46923	VBD	denotes	were
T10325	46924-46932	VBN	denotes	injected
T10336	46933-46951	RB	denotes	intra-peritoneally
T10337	46952-46956	IN	denotes	with
T10338	46957-46960	CD	denotes	100
T10339	46961-46963	NN	denotes	μg
T10341	46963-46964	SYM	denotes	/
T10342	46964-46966	NN	denotes	gm
T10343	46967-46971	NN	denotes	body
T10340	46972-46978	NN	denotes	weight
T10344	46979-46981	IN	denotes	of
T10345	46982-46986	NNP	denotes	BrdU
T10346	46987-46988	-LRB-	denotes	(
T10347	46988-46993	NNP	denotes	Sigma
T10348	46993-46995	,	denotes	,
T10349	46995-46997	NNP	denotes	St
T10350	46998-47003	NNP	denotes	Louis
T10351	47003-47005	,	denotes	,
T10352	47005-47007	NNP	denotes	MO
T10353	47007-47008	-RRB-	denotes	)
T10354	47009-47010	CD	denotes	2
T10355	47011-47012	NN	denotes	h
T10357	47013-47018	JJ	denotes	prior
T10356	47019-47021	IN	denotes	to
T10358	47022-47031	NN	denotes	sacrifice
T10359	47031-47033	,	denotes	,
T10360	47033-47040	NNS	denotes	embryos
T10335	47041-47050	VBN	denotes	dissected
T10361	47051-47055	IN	denotes	from
T10362	47056-47061	NNS	denotes	uteri
T10363	47062-47065	CC	denotes	and
T10364	47066-47075	VBN	denotes	processed
T10365	47076-47078	IN	denotes	as
T10366	47079-47088	VBN	denotes	described
T10367	47089-47094	RB	denotes	above
T10368	47094-47095	.	denotes	.
T10369	47095-47323	sentence	denotes	Sections were stained with a monoclonal anti-BrdU antibody (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA) and visualized with a Cy2-conjugated rabbit anti-mouse antibody using a laser-scanning confocal microscope.
T10370	47096-47104	NNS	denotes	Sections
T10372	47105-47109	VBD	denotes	were
T10371	47110-47117	VBN	denotes	stained
T10373	47118-47122	IN	denotes	with
T10374	47123-47124	DT	denotes	a
T10376	47125-47135	JJ	denotes	monoclonal
T10377	47136-47145	JJ	denotes	anti-BrdU
T10375	47146-47154	NN	denotes	antibody
T10378	47155-47156	-LRB-	denotes	(
T10380	47156-47157	CD	denotes	1
T10381	47157-47158	SYM	denotes	:
T10382	47158-47161	CD	denotes	100
T10383	47161-47163	,	denotes	,
T10384	47163-47176	NNP	denotes	Developmental
T10385	47177-47184	NNPS	denotes	Studies
T10386	47185-47194	NNP	denotes	Hybridoma
T10379	47195-47199	NNP	denotes	Bank
T10387	47199-47201	,	denotes	,
T10388	47201-47205	NNP	denotes	Iowa
T10389	47206-47210	NNP	denotes	City
T10390	47210-47212	,	denotes	,
T10391	47212-47214	NNP	denotes	IA
T10392	47214-47215	-RRB-	denotes	)
T10393	47216-47219	CC	denotes	and
T10394	47220-47230	VBN	denotes	visualized
T10395	47231-47235	IN	denotes	with
T10396	47236-47237	DT	denotes	a
T10398	47238-47241	NN	denotes	Cy2
T10400	47241-47242	HYPH	denotes	-
T10399	47242-47252	VBN	denotes	conjugated
T10401	47253-47259	NN	denotes	rabbit
T10402	47260-47270	JJ	denotes	anti-mouse
T10397	47271-47279	NN	denotes	antibody
T10403	47280-47285	VBG	denotes	using
T10404	47286-47287	DT	denotes	a
T10406	47288-47293	NN	denotes	laser
T10408	47293-47294	HYPH	denotes	-
T10407	47294-47302	VBG	denotes	scanning
T10409	47303-47311	JJ	denotes	confocal
T10405	47312-47322	NN	denotes	microscope
T10410	47322-47323	.	denotes	.
T10411	47323-47554	sentence	denotes	Cell death was investigated by terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL) using the Fluorescein In Situ Cell Death Detection Kit (Roche, Indianapolis, IN) according to the manufacturer's protocol.
T10412	47324-47328	NN	denotes	Cell
T10413	47329-47334	NN	denotes	death
T10415	47335-47338	VBD	denotes	was
T10414	47339-47351	VBN	denotes	investigated
T10416	47352-47354	IN	denotes	by
T10417	47355-47363	JJ	denotes	terminal
T10419	47364-47391	NN	denotes	deoxynucleotidyltransferase
T10421	47391-47392	HYPH	denotes	-
T10420	47392-47400	VBN	denotes	mediated
T10422	47401-47405	NN	denotes	dUTP
T10423	47406-47410	NN	denotes	nick
T10424	47410-47411	HYPH	denotes	-
T10418	47411-47414	NN	denotes	end
T10425	47415-47423	VBG	denotes	labeling
T10426	47424-47425	-LRB-	denotes	(
T10427	47425-47430	NN	denotes	TUNEL
T10428	47430-47431	-RRB-	denotes	)
T10429	47432-47437	VBG	denotes	using
T10430	47438-47441	DT	denotes	the
T10432	47442-47453	NNP	denotes	Fluorescein
T10433	47454-47456	FW	denotes	In
T10434	47457-47461	FW	denotes	Situ
T10435	47462-47466	NN	denotes	Cell
T10436	47467-47472	NN	denotes	Death
T10437	47473-47482	NN	denotes	Detection
T10431	47483-47486	NN	denotes	Kit
T10438	47487-47488	-LRB-	denotes	(
T10439	47488-47493	NNP	denotes	Roche
T10440	47493-47495	,	denotes	,
T10441	47495-47507	NNP	denotes	Indianapolis
T10442	47507-47509	,	denotes	,
T10443	47509-47511	NNP	denotes	IN
T10444	47511-47512	-RRB-	denotes	)
T10445	47513-47522	VBG	denotes	according
T10446	47523-47525	IN	denotes	to
T10447	47526-47529	DT	denotes	the
T10448	47530-47542	NN	denotes	manufacturer
T10450	47542-47544	POS	denotes	's
T10449	47545-47553	NN	denotes	protocol
T10451	47553-47554	.	denotes	.
T10545	47556-47563	NNP	denotes	Western
T10546	47564-47572	NN	denotes	blotting
T10547	47572-47690	sentence	denotes	Sample preparation and western blotting was performed according to Kawakami et al with some minor modifications [35].
T10548	47573-47579	NN	denotes	Sample
T10549	47580-47591	NN	denotes	preparation
T10551	47592-47595	CC	denotes	and
T10552	47596-47603	NNP	denotes	western
T10553	47604-47612	NN	denotes	blotting
T10554	47613-47616	VBD	denotes	was
T10550	47617-47626	VBN	denotes	performed
T10555	47627-47636	VBG	denotes	according
T10556	47637-47639	IN	denotes	to
T10557	47640-47648	NNP	denotes	Kawakami
T10558	47649-47651	FW	denotes	et
T10559	47652-47654	FW	denotes	al
T10560	47655-47659	IN	denotes	with
T10561	47660-47664	DT	denotes	some
T10563	47665-47670	JJ	denotes	minor
T10562	47671-47684	NNS	denotes	modifications
T10564	47685-47686	-LRB-	denotes	[
T10565	47686-47688	CD	denotes	35
T10566	47688-47689	-RRB-	denotes	]
T10567	47689-47690	.	denotes	.
T10568	47690-48072	sentence	denotes	Briefly, 3 embryos from each genotype were pooled, homogenized in ice cold lysis buffer (50 mM Tris-pH8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.5 mM DTT, 1 mM PMSF and 2 μg/ml each of aprotinin, leupeptin and pepstatin A) with a tissue homogenizer (PRO Scientific Inc., Oxford, CT) using three pulses of 10 seconds each and then subjected to Dounce homogenizer by 10 strokes.
T10569	47691-47698	RB	denotes	Briefly
T10571	47698-47700	,	denotes	,
T10572	47700-47701	CD	denotes	3
T10573	47702-47709	NNS	denotes	embryos
T10574	47710-47714	IN	denotes	from
T10575	47715-47719	DT	denotes	each
T10576	47720-47728	NN	denotes	genotype
T10577	47729-47733	VBD	denotes	were
T10570	47734-47740	VBN	denotes	pooled
T10578	47740-47742	,	denotes	,
T10579	47742-47753	VBN	denotes	homogenized
T10580	47754-47756	IN	denotes	in
T10581	47757-47760	NN	denotes	ice
T10582	47761-47765	JJ	denotes	cold
T10584	47766-47771	NN	denotes	lysis
T10583	47772-47778	NN	denotes	buffer
T10585	47779-47780	-LRB-	denotes	(
T10587	47780-47782	CD	denotes	50
T10588	47783-47785	NN	denotes	mM
T10589	47786-47790	NN	denotes	Tris
T10590	47790-47791	:	denotes	-
T10586	47791-47796	NN	denotes	pH8.0
T10591	47796-47798	,	denotes	,
T10592	47798-47801	CD	denotes	150
T10593	47802-47804	NN	denotes	mM
T10594	47805-47809	NN	denotes	NaCl
T10595	47809-47811	,	denotes	,
T10596	47811-47812	CD	denotes	2
T10597	47813-47815	NN	denotes	mM
T10598	47816-47820	NN	denotes	EDTA
T10599	47820-47822	,	denotes	,
T10600	47822-47823	CD	denotes	1
T10601	47823-47824	NN	denotes	%
T10603	47825-47831	NN	denotes	Triton
T10602	47832-47833	NN	denotes	X
T10604	47833-47834	HYPH	denotes	-
T10605	47834-47837	CD	denotes	100
T10606	47837-47839	,	denotes	,
T10607	47839-47842	CD	denotes	0.5
T10608	47843-47845	NN	denotes	mM
T10609	47846-47849	NN	denotes	DTT
T10610	47849-47851	,	denotes	,
T10611	47851-47852	CD	denotes	1
T10612	47853-47855	NN	denotes	mM
T10613	47856-47860	NN	denotes	PMSF
T10614	47861-47864	CC	denotes	and
T10615	47865-47866	CD	denotes	2
T10616	47867-47869	NN	denotes	μg
T10617	47869-47870	SYM	denotes	/
T10618	47870-47872	NN	denotes	ml
T10619	47873-47877	DT	denotes	each
T10620	47878-47880	IN	denotes	of
T10621	47881-47890	NN	denotes	aprotinin
T10622	47890-47892	,	denotes	,
T10623	47892-47901	NN	denotes	leupeptin
T10624	47902-47905	CC	denotes	and
T10625	47906-47915	NN	denotes	pepstatin
T10626	47916-47917	NN	denotes	A
T10627	47917-47918	-RRB-	denotes	)
T10628	47919-47923	IN	denotes	with
T10629	47924-47925	DT	denotes	a
T10631	47926-47932	NN	denotes	tissue
T10630	47933-47944	NN	denotes	homogenizer
T10632	47945-47946	-LRB-	denotes	(
T10634	47946-47949	NNP	denotes	PRO
T10635	47950-47960	NNP	denotes	Scientific
T10633	47961-47965	NNP	denotes	Inc.
T10636	47965-47967	,	denotes	,
T10637	47967-47973	NNP	denotes	Oxford
T10638	47973-47975	,	denotes	,
T10639	47975-47977	NNP	denotes	CT
T10640	47977-47978	-RRB-	denotes	)
T10641	47979-47984	VBG	denotes	using
T10642	47985-47990	CD	denotes	three
T10643	47991-47997	NNS	denotes	pulses
T10644	47998-48000	IN	denotes	of
T10645	48001-48003	CD	denotes	10
T10646	48004-48011	NNS	denotes	seconds
T10647	48012-48016	DT	denotes	each
T10648	48017-48020	CC	denotes	and
T10649	48021-48025	RB	denotes	then
T10650	48026-48035	VBN	denotes	subjected
T10651	48036-48038	IN	denotes	to
T10652	48039-48045	NNP	denotes	Dounce
T10653	48046-48057	NN	denotes	homogenizer
T10654	48058-48060	IN	denotes	by
T10655	48061-48063	CD	denotes	10
T10656	48064-48071	NNS	denotes	strokes
T10657	48071-48072	.	denotes	.
T10658	48072-48228	sentence	denotes	The homogenates were centrifuged at 10,000 g for 30 minutes at 4°C, supernatants collected, protein concentration determined and used for western blotting.
T10659	48073-48076	DT	denotes	The
T10660	48077-48088	NNS	denotes	homogenates
T10662	48089-48093	VBD	denotes	were
T10661	48094-48105	VBN	denotes	centrifuged
T10664	48106-48108	IN	denotes	at
T10665	48109-48115	CD	denotes	10,000
T10666	48116-48117	NN	denotes	g
T10667	48118-48121	IN	denotes	for
T10668	48122-48124	CD	denotes	30
T10669	48125-48132	NNS	denotes	minutes
T10670	48133-48135	IN	denotes	at
T10671	48136-48137	CD	denotes	4
T10672	48137-48139	NN	denotes	°C
T10673	48139-48141	,	denotes	,
T10674	48141-48153	NNS	denotes	supernatants
T10675	48154-48163	VBN	denotes	collected
T10676	48163-48165	,	denotes	,
T10677	48165-48172	NN	denotes	protein
T10678	48173-48186	NN	denotes	concentration
T10663	48187-48197	VBN	denotes	determined
T10679	48198-48201	CC	denotes	and
T10680	48202-48206	VBN	denotes	used
T10681	48207-48210	IN	denotes	for
T10682	48211-48218	NNP	denotes	western
T10683	48219-48227	NN	denotes	blotting
T10684	48227-48228	.	denotes	.
T10685	48228-48673	sentence	denotes	COS1 cells were transfected using Superfect transfection reagent (Qiagen, Valencia, CA) with expression constructs encoding either the full-length Shh or the N-terminal fragment of Shh without post-translational modifications (constructs kindly provided by Dr. Pao-Tien Chuang, Cardiovascular Research Institute, University of California, San Francisco, CA), harvested 3 days after transfection and protein extracts prepared as described above.
T10686	48229-48233	NN	denotes	COS1
T10687	48234-48239	NNS	denotes	cells
T10689	48240-48244	VBD	denotes	were
T10688	48245-48256	VBN	denotes	transfected
T10690	48257-48262	VBG	denotes	using
T10691	48263-48272	NNP	denotes	Superfect
T10693	48273-48285	NN	denotes	transfection
T10692	48286-48293	NN	denotes	reagent
T10694	48294-48295	-LRB-	denotes	(
T10695	48295-48301	NNP	denotes	Qiagen
T10696	48301-48303	,	denotes	,
T10697	48303-48311	NNP	denotes	Valencia
T10698	48311-48313	,	denotes	,
T10699	48313-48315	NNP	denotes	CA
T10700	48315-48316	-RRB-	denotes	)
T10701	48317-48321	IN	denotes	with
T10702	48322-48332	NN	denotes	expression
T10703	48333-48343	NNS	denotes	constructs
T10704	48344-48352	VBG	denotes	encoding
T10705	48353-48359	CC	denotes	either
T10707	48360-48363	DT	denotes	the
T10708	48364-48368	JJ	denotes	full
T10710	48368-48369	HYPH	denotes	-
T10709	48369-48375	NN	denotes	length
T10706	48376-48379	NN	denotes	Shh
T10711	48380-48382	CC	denotes	or
T10712	48383-48386	DT	denotes	the
T10714	48387-48388	NN	denotes	N
T10716	48388-48389	HYPH	denotes	-
T10715	48389-48397	JJ	denotes	terminal
T10713	48398-48406	NN	denotes	fragment
T10717	48407-48409	IN	denotes	of
T10718	48410-48413	NN	denotes	Shh
T10719	48414-48421	IN	denotes	without
T10720	48422-48440	JJ	denotes	post-translational
T10721	48441-48454	NNS	denotes	modifications
T10722	48455-48456	-LRB-	denotes	(
T10724	48456-48466	NNS	denotes	constructs
T10725	48467-48473	RB	denotes	kindly
T10723	48474-48482	VBN	denotes	provided
T10726	48483-48485	IN	denotes	by
T10727	48486-48489	NNP	denotes	Dr.
T10729	48490-48493	NNP	denotes	Pao
T10731	48493-48494	HYPH	denotes	-
T10730	48494-48498	NNP	denotes	Tien
T10728	48499-48505	NNP	denotes	Chuang
T10732	48505-48507	,	denotes	,
T10734	48507-48521	NNP	denotes	Cardiovascular
T10735	48522-48530	NNP	denotes	Research
T10733	48531-48540	NNP	denotes	Institute
T10736	48540-48542	,	denotes	,
T10737	48542-48552	NNP	denotes	University
T10738	48553-48555	IN	denotes	of
T10739	48556-48566	NNP	denotes	California
T10740	48566-48568	,	denotes	,
T10741	48568-48571	NNP	denotes	San
T10742	48572-48581	NNP	denotes	Francisco
T10743	48581-48583	,	denotes	,
T10744	48583-48585	NNP	denotes	CA
T10745	48585-48586	-RRB-	denotes	)
T10746	48586-48588	,	denotes	,
T10747	48588-48597	VBD	denotes	harvested
T10748	48598-48599	CD	denotes	3
T10749	48600-48604	NNS	denotes	days
T10750	48605-48610	IN	denotes	after
T10751	48611-48623	NN	denotes	transfection
T10753	48624-48627	CC	denotes	and
T10754	48628-48635	NN	denotes	protein
T10752	48636-48644	NNS	denotes	extracts
T10755	48645-48653	VBN	denotes	prepared
T10756	48654-48656	IN	denotes	as
T10757	48657-48666	VBN	denotes	described
T10758	48667-48672	RB	denotes	above
T10759	48672-48673	.	denotes	.
T10760	48673-49133	sentence	denotes	Protein concentration was determined using Bio-Rad protein assay kit (BioRad, Hercules, CA) and 20 μg (COS1 cells) and 75 μg (embryos) of lysates separated by 15 % SDS-PAGE, transferred onto nitrocellulose membrane, incubated with primary antibody (Shh-N19, 1:100) for 2 hours, followed by donkey anti-goat HRP second antibody (1:1000) and visualized by chemiluminescent detection according to manufacturer's protocols (Perkin-Elmer Life Sciences, Boston MA).
T10761	48674-48681	NN	denotes	Protein
T10762	48682-48695	NN	denotes	concentration
T10764	48696-48699	VBD	denotes	was
T10763	48700-48710	VBN	denotes	determined
T10765	48711-48716	VBG	denotes	using
T10766	48717-48720	NNP	denotes	Bio
T10768	48720-48721	HYPH	denotes	-
T10767	48721-48724	NNP	denotes	Rad
T10770	48725-48732	NN	denotes	protein
T10771	48733-48738	NN	denotes	assay
T10769	48739-48742	NN	denotes	kit
T10772	48743-48744	-LRB-	denotes	(
T10773	48744-48750	NNP	denotes	BioRad
T10774	48750-48752	,	denotes	,
T10775	48752-48760	NNP	denotes	Hercules
T10776	48760-48762	,	denotes	,
T10777	48762-48764	NNP	denotes	CA
T10778	48764-48765	-RRB-	denotes	)
T10779	48766-48769	CC	denotes	and
T10780	48770-48772	CD	denotes	20
T10781	48773-48775	NN	denotes	μg
T10782	48776-48777	-LRB-	denotes	(
T10784	48777-48781	NN	denotes	COS1
T10783	48782-48787	NNS	denotes	cells
T10785	48787-48788	-RRB-	denotes	)
T10786	48789-48792	CC	denotes	and
T10787	48793-48795	CD	denotes	75
T10788	48796-48798	NN	denotes	μg
T10789	48799-48800	-LRB-	denotes	(
T10790	48800-48807	NNS	denotes	embryos
T10791	48807-48808	-RRB-	denotes	)
T10792	48809-48811	IN	denotes	of
T10793	48812-48819	NNS	denotes	lysates
T10794	48820-48829	VBN	denotes	separated
T10795	48830-48832	IN	denotes	by
T10796	48833-48835	CD	denotes	15
T10797	48836-48837	NN	denotes	%
T10799	48838-48841	NN	denotes	SDS
T10800	48841-48842	HYPH	denotes	-
T10798	48842-48846	NN	denotes	PAGE
T10801	48846-48848	,	denotes	,
T10802	48848-48859	VBN	denotes	transferred
T10803	48860-48864	IN	denotes	onto
T10804	48865-48879	NN	denotes	nitrocellulose
T10805	48880-48888	NN	denotes	membrane
T10806	48888-48890	,	denotes	,
T10807	48890-48899	VBN	denotes	incubated
T10808	48900-48904	IN	denotes	with
T10809	48905-48912	JJ	denotes	primary
T10810	48913-48921	NN	denotes	antibody
T10811	48922-48923	-LRB-	denotes	(
T10813	48923-48926	NN	denotes	Shh
T10815	48926-48927	HYPH	denotes	-
T10814	48927-48930	NN	denotes	N19
T10816	48930-48932	,	denotes	,
T10812	48932-48933	CD	denotes	1
T10817	48933-48934	SYM	denotes	:
T10818	48934-48937	CD	denotes	100
T10819	48937-48938	-RRB-	denotes	)
T10820	48939-48942	IN	denotes	for
T10821	48943-48944	CD	denotes	2
T10822	48945-48950	NNS	denotes	hours
T10823	48950-48952	,	denotes	,
T10824	48952-48960	VBN	denotes	followed
T10825	48961-48963	IN	denotes	by
T10826	48964-48970	NN	denotes	donkey
T10828	48971-48980	JJ	denotes	anti-goat
T10829	48981-48984	NN	denotes	HRP
T10830	48985-48991	JJ	denotes	second
T10827	48992-49000	NN	denotes	antibody
T10831	49001-49002	-LRB-	denotes	(
T10832	49002-49003	CD	denotes	1
T10833	49003-49004	SYM	denotes	:
T10834	49004-49008	CD	denotes	1000
T10835	49008-49009	-RRB-	denotes	)
T10836	49010-49013	CC	denotes	and
T10837	49014-49024	VBN	denotes	visualized
T10838	49025-49027	IN	denotes	by
T10839	49028-49044	JJ	denotes	chemiluminescent
T10840	49045-49054	NN	denotes	detection
T10841	49055-49064	VBG	denotes	according
T10842	49065-49067	IN	denotes	to
T10843	49068-49080	NN	denotes	manufacturer
T10845	49080-49082	POS	denotes	's
T10844	49083-49092	NNS	denotes	protocols
T10846	49093-49094	-LRB-	denotes	(
T10848	49094-49100	NNP	denotes	Perkin
T10850	49100-49101	HYPH	denotes	-
T10849	49101-49106	NNP	denotes	Elmer
T10851	49107-49111	NNP	denotes	Life
T10847	49112-49120	NNPS	denotes	Sciences
T10852	49120-49122	,	denotes	,
T10853	49122-49128	NNP	denotes	Boston
T10854	49129-49131	NNP	denotes	MA
T10855	49131-49132	-RRB-	denotes	)
T10856	49132-49133	.	denotes	.
T10893	49135-49143	NNP	denotes	Northern
T10894	49144-49152	NN	denotes	blotting
T10895	49152-49258	sentence	denotes	Northern blotting was performed as previously described [46], using total RNA (20 μg) from P0 mice lungs.
T10896	49153-49161	NNP	denotes	Northern
T10897	49162-49170	NN	denotes	blotting
T10899	49171-49174	VBD	denotes	was
T10898	49175-49184	VBN	denotes	performed
T10900	49185-49187	IN	denotes	as
T10902	49188-49198	RB	denotes	previously
T10901	49199-49208	VBN	denotes	described
T10903	49209-49210	-LRB-	denotes	[
T10904	49210-49212	CD	denotes	46
T10905	49212-49213	-RRB-	denotes	]
T10906	49213-49215	,	denotes	,
T10907	49215-49220	VBG	denotes	using
T10908	49221-49226	JJ	denotes	total
T10909	49227-49230	NN	denotes	RNA
T10910	49231-49232	-LRB-	denotes	(
T10912	49232-49234	CD	denotes	20
T10911	49235-49237	NN	denotes	μg
T10913	49237-49238	-RRB-	denotes	)
T10914	49239-49243	IN	denotes	from
T10915	49244-49246	NN	denotes	P0
T10916	49247-49251	NNS	denotes	mice
T10917	49252-49257	NNS	denotes	lungs
T10918	49257-49258	.	denotes	.
T10919	49258-49441	sentence	denotes	The cDNA probes for SP-A, SP-B and SP-C were kindly provided by Dr. Jeffery Whitsett (Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio).
T10920	49259-49262	DT	denotes	The
T10922	49263-49267	NN	denotes	cDNA
T10921	49268-49274	NNS	denotes	probes
T10924	49275-49278	IN	denotes	for
T10925	49279-49281	NN	denotes	SP
T10927	49281-49282	HYPH	denotes	-
T10926	49282-49283	NN	denotes	A
T10928	49283-49285	,	denotes	,
T10929	49285-49287	NN	denotes	SP
T10931	49287-49288	HYPH	denotes	-
T10930	49288-49289	NN	denotes	B
T10932	49290-49293	CC	denotes	and
T10933	49294-49296	NN	denotes	SP
T10935	49296-49297	HYPH	denotes	-
T10934	49297-49298	NN	denotes	C
T10936	49299-49303	VBD	denotes	were
T10937	49304-49310	RB	denotes	kindly
T10923	49311-49319	VBN	denotes	provided
T10938	49320-49322	IN	denotes	by
T10939	49323-49326	NNP	denotes	Dr.
T10941	49327-49334	NNP	denotes	Jeffery
T10940	49335-49343	NNP	denotes	Whitsett
T10942	49344-49345	-LRB-	denotes	(
T10943	49345-49353	NNP	denotes	Division
T10944	49354-49356	IN	denotes	of
T10945	49357-49366	NNP	denotes	Pulmonary
T10946	49367-49374	NNP	denotes	Biology
T10947	49374-49376	,	denotes	,
T10948	49376-49386	NNP	denotes	Cincinnati
T10950	49387-49395	NNPS	denotes	Children
T10951	49395-49397	POS	denotes	's
T10949	49398-49406	NNP	denotes	Hospital
T10953	49407-49414	NNP	denotes	Medical
T10952	49415-49421	NNP	denotes	Center
T10954	49421-49423	,	denotes	,
T10955	49423-49433	NNP	denotes	Cincinnati
T10956	49433-49435	,	denotes	,
T10957	49435-49439	NNP	denotes	Ohio
T10958	49439-49440	-RRB-	denotes	)
T10959	49440-49441	.	denotes	.
T10960	49441-49633	sentence	denotes	A cDNA probe for SP-D was amplified by RT-PCR, using murine SP-D cDNA specific primers (sense 5'-TGCCTGGTCGTGATGGACG-3', and antisense 5'-AGCACTGTCTGGAAGCCCGC-3') and confirmed by sequencing.
T10961	49442-49443	DT	denotes	A
T10963	49444-49448	NN	denotes	cDNA
T10962	49449-49454	NN	denotes	probe
T10965	49455-49458	IN	denotes	for
T10966	49459-49461	NN	denotes	SP
T10968	49461-49462	HYPH	denotes	-
T10967	49462-49463	NN	denotes	D
T10969	49464-49467	VBD	denotes	was
T10964	49468-49477	VBN	denotes	amplified
T10970	49478-49480	IN	denotes	by
T10971	49481-49483	NN	denotes	RT
T10973	49483-49484	HYPH	denotes	-
T10972	49484-49487	NN	denotes	PCR
T10974	49487-49489	,	denotes	,
T10975	49489-49494	VBG	denotes	using
T10976	49495-49501	JJ	denotes	murine
T10978	49502-49504	NN	denotes	SP
T10980	49504-49505	HYPH	denotes	-
T10979	49505-49506	NN	denotes	D
T10981	49507-49511	NN	denotes	cDNA
T10982	49512-49520	JJ	denotes	specific
T10977	49521-49528	NNS	denotes	primers
T10983	49529-49530	-LRB-	denotes	(
T10985	49530-49535	NN	denotes	sense
T10986	49536-49537	CD	denotes	5
T10987	49537-49538	SYM	denotes	'
T10988	49538-49539	HYPH	denotes	-
T10984	49539-49558	NN	denotes	TGCCTGGTCGTGATGGACG
T10989	49558-49559	HYPH	denotes	-
T10990	49559-49560	CD	denotes	3
T10991	49560-49561	SYM	denotes	'
T10992	49561-49563	,	denotes	,
T10993	49563-49566	CC	denotes	and
T10994	49567-49576	JJ	denotes	antisense
T10996	49577-49578	CD	denotes	5
T10997	49578-49579	SYM	denotes	'
T10998	49579-49580	HYPH	denotes	-
T10995	49580-49600	NN	denotes	AGCACTGTCTGGAAGCCCGC
T10999	49600-49601	HYPH	denotes	-
T11000	49601-49602	CD	denotes	3
T11001	49602-49603	SYM	denotes	'
T11002	49603-49604	-RRB-	denotes	)
T11003	49605-49608	CC	denotes	and
T11004	49609-49618	VBN	denotes	confirmed
T11005	49619-49621	IN	denotes	by
T11006	49622-49632	NN	denotes	sequencing
T11007	49632-49633	.	denotes	.
R10	T248	T247	compound	mouse,model
R100	T351	T350	prep	of,development
R1000	T1803	T1801	amod	distal,to
R1001	T1804	T1799	prep	of,differentiation
R1002	T1805	T1806	compound	lung,epithelium
R1003	T1806	T1804	pobj	epithelium,of
R1004	T1807	T1808	punct	[,24
R1005	T1808	T1797	parataxis	24,preserved
R1006	T1809	T1808	punct	],24
R1007	T1810	T1781	punct	.,resulted
R1008	T1865	T1863	pobj	failure,to
R1009	T1812	T1813	nsubj	Overexpression,resulted
R101	T352	T353	det	these,mice
R1010	T1866	T1850	punct	.,died
R1011	T1814	T1812	prep	of,Overexpression
R1012	T1868	T1869	nsubjpass	It,reported
R1013	T1870	T1869	aux	has,reported
R1014	T1815	T1814	pobj	Shh,of
R1015	T1871	T1869	auxpass	been,reported
R1016	T1872	T1873	mark	that,led
R1017	T1816	T1812	prep	in,Overexpression
R1018	T1817	T1818	det	the,epithelium
R1019	T1873	T1869	ccomp	led,reported
R102	T353	T351	pobj	mice,of
R1020	T1874	T1873	nsubj	inhibition,led
R1021	T1818	T1816	pobj	epithelium,in
R1022	T1875	T1874	prep	of,inhibition
R1023	T1876	T1877	compound	cholesterol,biosynthesis
R1024	T1819	T1818	amod	distal,epithelium
R1025	T1877	T1875	pobj	biosynthesis,of
R1026	T1878	T1874	prep	by,inhibition
R1027	T1879	T1880	amod	chemical,inhibitors
R1028	T1820	T1818	compound	lung,epithelium
R1029	T1880	T1878	pobj	inhibitors,by
R103	T354	T337	punct	.,characterized
R1030	T1881	T1880	prep	of,inhibitors
R1031	T1882	T1881	pobj	Dhcr7,of
R1032	T1821	T1813	prep	in,resulted
R1033	T1883	T1873	advmod	also,led
R1034	T1884	T1873	prep	to,led
R1035	T1885	T1886	det	a,disturbance
R1036	T1822	T1823	det	the,absence
R1037	T1886	T1884	pobj	disturbance,to
R1038	T1887	T1886	prep	of,disturbance
R1039	T1823	T1821	pobj	absence,in
R104	T356	T357	amod	Significant,retardation
R1040	T1888	T1889	det	the,signaling
R1041	T1889	T1887	pobj	signaling,of
R1042	T1824	T1823	prep	of,absence
R1043	T1890	T1889	compound	Shh,signaling
R1044	T1891	T1889	prep	with,signaling
R1045	T1892	T1893	amod	abnormal,embryogenesis
R1046	T1825	T1826	amod	functional,alveoli
R1047	T1893	T1891	pobj	embryogenesis,with
R1048	T1894	T1895	punct	[,28
R1049	T1895	T1873	parataxis	28,led
R105	T357	T359	nsubjpass	retardation,observed
R1050	T1896	T1895	nummod	27,28
R1051	T1826	T1824	pobj	alveoli,of
R1052	T1827	T1823	cc	and,absence
R1053	T1897	T1895	punct	",",28
R1054	T1898	T1895	punct	],28
R1055	T1899	T1869	punct	", ",reported
R1056	T1900	T1869	cc	and,reported
R1057	T1901	T1902	amod	severe,deprivation
R1058	T1902	T1904	nsubj	deprivation,inhibit
R1059	T1903	T1902	compound	sterol,deprivation
R106	T358	T357	compound	growth,retardation
R1060	T1828	T1829	det	an,increase
R1061	T1904	T1869	conj	inhibit,reported
R1062	T1905	T1904	aux	can,inhibit
R1063	T1906	T1904	advmod	indeed,inhibit
R1064	T1907	T1908	det	the,processing
R1065	T1829	T1823	conj	increase,absence
R1066	T1908	T1904	dobj	processing,inhibit
R1067	T1909	T1908	prep	of,processing
R1068	T1910	T1911	amod	transfected,Shh
R1069	T1830	T1829	prep	in,increase
R107	T360	T357	punct	", ",retardation
R1070	T1911	T1909	pobj	Shh,of
R1071	T1912	T1908	prep	in,processing
R1072	T1831	T1832	amod	interstitial,tissue
R1073	T1913	T1914	amod	cultured,cells
R1074	T1914	T1912	pobj	cells,in
R1075	T1915	T1914	amod	mammalian,cells
R1076	T1832	T1830	pobj	tissue,in
R1077	T1916	T1917	punct	[,29
R1078	T1917	T1904	parataxis	29,inhibit
R1079	T1833	T1829	acl	caused,increase
R108	T361	T357	acl	beginning,retardation
R1080	T1918	T1917	punct	],29
R1081	T1919	T1904	punct	.,inhibit
R1082	T1834	T1833	agent	by,caused
R1083	T1921	T1922	prep	In,characterized
R1084	T1923	T1924	det	the,studies
R1085	T1835	T1836	det	an,proliferation
R1086	T1924	T1921	pobj	studies,In
R1087	T1836	T1834	pobj	proliferation,by
R1088	T1925	T1924	amod	present,studies
R1089	T1926	T1922	punct	", ",characterized
R109	T362	T361	prep	at,beginning
R1090	T1837	T1836	amod	increased,proliferation
R1091	T1927	T1922	nsubj	we,characterized
R1092	T1928	T1929	compound	lung,development
R1093	T1929	T1922	dobj	development,characterized
R1094	T1838	T1836	prep	of,proliferation
R1095	T1930	T1929	prep	in,development
R1096	T1931	T1932	nmod	Dhcr7,embryos
R1097	T1932	T1930	pobj	embryos,in
R1098	T1839	T1840	preconj	both,epithelial
R1099	T1933	T1931	punct	-,Dhcr7
R11	T249	T247	prep	of,model
R110	T363	T362	pobj	E14.5,at
R1100	T1934	T1931	punct	/,Dhcr7
R1101	T1935	T1931	punct	-,Dhcr7
R1102	T1840	T1841	amod	epithelial,cells
R1103	T1936	T1922	prep	from,characterized
R1104	T1937	T1936	pobj	E10.5,from
R1105	T1841	T1838	pobj	cells,of
R1106	T1938	T1936	prep	to,from
R1107	T1842	T1840	cc	and,epithelial
R1108	T1939	T1938	pobj	birth,to
R1109	T1940	T1922	punct	.,characterized
R111	T364	T365	punct	~,E16.5
R1110	T1942	T1943	poss	Our,results
R1111	T1943	T1944	nsubj	results,showed
R1112	T1945	T1946	mark	that,alter
R1113	T1843	T1840	conj	mesenchyme,epithelial
R1114	T1946	T1944	ccomp	alter,showed
R1115	T1947	T1948	compound	cholesterol,deficiency
R1116	T1948	T1946	nsubj	deficiency,alter
R1117	T1844	T1845	punct	[,26
R1118	T1949	T1948	punct	", ",deficiency
R1119	T1950	T1948	acl	caused,deficiency
R112	T365	T363	prep	E16.5,E14.5
R1120	T1951	T1950	agent	by,caused
R1121	T1845	T1813	parataxis	26,resulted
R1122	T1952	T1951	pobj	loss,by
R1123	T1953	T1952	prep	of,loss
R1124	T1954	T1955	compound	Dhcr7,activity
R1125	T1846	T1845	punct	],26
R1126	T1955	T1953	pobj	activity,of
R1127	T1956	T1946	punct	", ",alter
R1128	T1957	T1946	aux	did,alter
R1129	T1847	T1813	punct	.,resulted
R113	T366	T359	punct	", ",observed
R1130	T1958	T1946	neg	not,alter
R1131	T1959	T1960	compound	lung,branching
R1132	T1960	T1961	compound	branching,morphogenesis
R1133	T1849	T1850	nsubj	Mice,died
R1134	T1961	T1946	dobj	morphogenesis,alter
R1135	T1962	T1963	punct	(,stages
R1136	T1851	T1849	prep	with,Mice
R1137	T1963	T1946	parataxis	stages,alter
R1138	T1964	T1963	amod	early,stages
R1139	T1965	T1963	amod	gestational,stages
R114	T367	T359	auxpass	was,observed
R1140	T1852	T1853	compound	Shh,overexpression
R1141	T1966	T1963	punct	", ",stages
R1142	T1967	T1963	appos	E9.5,stages
R1143	T1968	T1967	prep	to,E9.5
R1144	T1969	T1968	pobj	E14.5,to
R1145	T1853	T1851	pobj	overexpression,with
R1146	T1970	T1963	punct	),stages
R1147	T1854	T1855	amod	less,24
R1148	T1855	T1857	nummod	24,h
R1149	T1856	T1855	quantmod	than,24
R115	T368	T359	prep	in,observed
R1150	T1857	T1858	npadvmod	h,after
R1151	T1971	T1944	punct	.,showed
R1152	T1858	T1850	prep	after,died
R1153	T1973	T1974	advmod	However,impaired
R1154	T1859	T1858	pobj	birth,after
R1155	T1975	T1974	punct	", ",impaired
R1156	T1860	T1850	punct	", ",died
R1157	T1976	T1977	nmod	lung,development
R1158	T1977	T1974	nsubjpass	development,impaired
R1159	T1861	T1862	advmod	probably,owing
R116	T369	T370	nmod	Dhcr7,embryos
R1160	T1978	T1977	amod	saccular,development
R1161	T1979	T1974	auxpass	was,impaired
R1162	T1980	T1974	punct	", ",impaired
R1163	T1981	T1974	prep	with,impaired
R1164	T1982	T1983	amod	arrested,tubules
R1165	T1862	T1850	advcl	owing,died
R1166	T1983	T1981	pobj	tubules,with
R1167	T1984	T1982	cc	or,arrested
R1168	T1985	T1986	advmod	partially,developed
R1169	T1863	T1862	prep	to,owing
R117	T370	T368	pobj	embryos,in
R1170	T1986	T1982	conj	developed,arrested
R1171	T1987	T1983	amod	epithelial,tubules
R1172	T1988	T1983	cc	and,tubules
R1173	T1864	T1865	amod	respiratory,failure
R1174	T1989	T1990	amod	delayed,development
R1175	T1990	T1983	conj	development,tubules
R1176	T1991	T1990	amod	vascular,development
R1177	T2077	T2075	punct	-,Dhcr7
R1178	T1992	T1974	punct	.,impaired
R1179	T1994	T1995	nsubj	Differentiation,appeared
R118	T371	T369	punct	-,Dhcr7
R1180	T2078	T2075	punct	/,Dhcr7
R1181	T1996	T1994	prep	of,Differentiation
R1182	T1997	T1996	pobj	type,of
R1183	T1998	T1997	nummod	II,type
R1184	T1999	T2000	amod	alveolar,cells
R1185	T2079	T2075	punct	-,Dhcr7
R1186	T2000	T1997	appos	cells,type
R1187	T2001	T2000	amod	epithelial,cells
R1188	T2002	T2000	punct	(,cells
R1189	T2080	T2047	punct	.,support
R119	T372	T369	punct	/,Dhcr7
R1190	T2003	T2000	appos	AECs,cells
R1191	T2004	T1994	punct	),Differentiation
R1192	T2082	T2083	nsubjpass	Patterns,altered
R1193	T2005	T1995	oprd	normal,appeared
R1194	T2006	T1995	cc	but,appeared
R1195	T2084	T2082	prep	of,Patterns
R1196	T2007	T2008	nsubjpass	differentiation,impaired
R1197	T2085	T2084	pobj	expression,of
R1198	T2008	T1995	conj	impaired,appeared
R1199	T2009	T2007	prep	of,differentiation
R12	T250	T251	det	the,syndrome
R120	T373	T369	punct	-,Dhcr7
R1200	T2010	T2011	nmod	type,AECs
R1201	T2086	T2085	prep	of,expression
R1202	T2011	T2009	pobj	AECs,of
R1203	T2012	T2010	nummod	I,type
R1204	T2087	T2088	compound	Shh,cascade
R1205	T2013	T2008	auxpass	was,impaired
R1206	T2014	T2008	advmod	severely,impaired
R1207	T2015	T2008	punct	.,impaired
R1208	T2088	T2090	compound	cascade,members
R1209	T2017	T2018	advmod	Therefore,resulted
R121	T374	T359	punct	.,observed
R1210	T2089	T2088	compound	signaling,cascade
R1211	T2019	T2018	punct	", ",resulted
R1212	T2020	T2021	compound	cholesterol,deficiency
R1213	T2021	T2018	nsubj	deficiency,resulted
R1214	T2022	T2018	prep	in,resulted
R1215	T2023	T2024	det	a,hypoplasia
R1216	T2024	T2022	pobj	hypoplasia,in
R1217	T2090	T2086	pobj	members,of
R1218	T2025	T2024	amod	distinct,hypoplasia
R1219	T2026	T2027	amod	late,gestational
R122	T376	T377	amod	Normal,lobation
R1220	T2091	T2083	auxpass	were,altered
R1221	T2027	T2024	amod	gestational,hypoplasia
R1222	T2028	T2024	compound	lung,hypoplasia
R1223	T2029	T2024	punct	", ",hypoplasia
R1224	T2092	T2083	neg	not,altered
R1225	T2030	T2024	acl	characterized,hypoplasia
R1226	T2031	T2030	agent	by,characterized
R1227	T2032	T2033	amod	impaired,sacculation
R1228	T2093	T2083	punct	", ",altered
R1229	T2033	T2031	pobj	sacculation,by
R123	T377	T378	nsubjpass	lobation,noted
R1230	T2034	T2033	prep	with,sacculation
R1231	T2035	T2036	amod	delayed,differentiation
R1232	T2094	T2083	cc	and,altered
R1233	T2036	T2034	pobj	differentiation,with
R1234	T2037	T2038	nmod	type,AECs
R1235	T2038	T2036	compound	AECs,differentiation
R1236	T2039	T2037	nummod	I,type
R1237	T2095	T2096	nsubj	processing,appeared
R1238	T2040	T2036	cc	and,differentiation
R1239	T2041	T2042	amod	immature,development
R124	T379	T377	cc	but,lobation
R1240	T2042	T2036	conj	development,differentiation
R1241	T2096	T2083	conj	appeared,altered
R1242	T2043	T2042	amod	vascular,development
R1243	T2044	T2018	punct	.,resulted
R1244	T2097	T2095	prep	of,processing
R1245	T2046	T2047	advmod	However,support
R1246	T2098	T2097	pobj	Shh,of
R1247	T2048	T2047	punct	", ",support
R1248	T2049	T2050	det	this,study
R1249	T2050	T2047	nsubj	study,support
R125	T380	T381	amod	smaller,lungs
R1250	T2099	T2096	oprd	normal,appeared
R1251	T2051	T2047	aux	does,support
R1252	T2052	T2047	neg	not,support
R1253	T2100	T2096	punct	.,appeared
R1254	T2053	T2054	det	the,concept
R1255	T2054	T2047	dobj	concept,support
R1256	T2055	T2056	mark	that,involved
R1257	T2056	T2054	acl	involved,concept
R1258	T2057	T2058	det	the,perturbation
R1259	T2058	T2056	nsubjpass	perturbation,involved
R126	T381	T377	conj	lungs,lobation
R1260	T2059	T2058	prep	of,perturbation
R1261	T2060	T2061	npadvmod	Shh,Patched
R1262	T2061	T2063	amod	Patched,Gli
R1263	T2062	T2061	punct	-,Patched
R1264	T2063	T2065	compound	Gli,pathway
R1265	T2064	T2063	punct	-,Gli
R1266	T2065	T2059	pobj	pathway,of
R1267	T2066	T2056	auxpass	was,involved
R1268	T2067	T2056	prep	in,involved
R1269	T2068	T2069	det	the,pathogenesis
R127	T382	T381	prep	with,lungs
R1270	T2069	T2067	pobj	pathogenesis,in
R1271	T2070	T2069	prep	of,pathogenesis
R1272	T2071	T2072	amod	developmental,hypoplasia
R1273	T2072	T2070	pobj	hypoplasia,of
R1274	T2073	T2072	compound	lung,hypoplasia
R1275	T2074	T2056	prep	in,involved
R1276	T2075	T2076	nmod	Dhcr7,embryos
R1277	T2076	T2074	pobj	embryos,in
R1278	T2191	T2192	compound	Body,growth
R1279	T2192	T2193	compound	growth,retardation
R128	T383	T384	det	a,decrease
R1280	T2194	T2193	prep	at,retardation
R1281	T2195	T2196	amod	late,stage
R1282	T2196	T2194	pobj	stage,at
R1283	T2197	T2196	compound	gestation,stage
R1284	T2198	T2193	prep	in,retardation
R1285	T2199	T2200	nmod	Dhcr7,embryos
R1286	T2200	T2198	pobj	embryos,in
R1287	T2201	T2199	punct	-,Dhcr7
R1288	T2202	T2199	punct	/,Dhcr7
R1289	T2203	T2199	punct	-,Dhcr7
R129	T384	T382	pobj	decrease,with
R1290	T2205	T2206	nmod	Dhcr7,mice
R1291	T2206	T2210	nsubjpass	mice,born
R1292	T2207	T2205	punct	-,Dhcr7
R1293	T2208	T2205	punct	/,Dhcr7
R1294	T2209	T2205	punct	-,Dhcr7
R1295	T2211	T2210	auxpass	were,born
R1296	T2212	T2210	prep	in,born
R1297	T2213	T2214	det	the,ratios
R1298	T2214	T2212	pobj	ratios,in
R1299	T2215	T2214	amod	expected,ratios
R13	T251	T249	pobj	syndrome,of
R130	T385	T384	amod	significant,decrease
R1300	T2216	T2214	amod	Mendelian,ratios
R1301	T2217	T2210	prep	from,born
R1302	T2218	T2219	amod	heterozygous,parents
R1303	T2219	T2217	pobj	parents,from
R1304	T2220	T2210	punct	", ",born
R1305	T2221	T2210	cc	but,born
R1306	T2222	T2223	nsubj	all,died
R1307	T2223	T2210	conj	died,born
R1308	T2224	T2222	prep	of,all
R1309	T2225	T2226	det	these,pups
R131	T386	T384	prep	in,decrease
R1310	T2226	T2224	pobj	pups,of
R1311	T2227	T2223	prep	within,died
R1312	T2228	T2229	nummod	24,h
R1313	T2229	T2227	pobj	h,within
R1314	T2230	T2223	cc	and,died
R1315	T2231	T2223	conj	most,died
R1316	T2232	T2231	prep	within,most
R1317	T2233	T2234	nummod	14,h
R1318	T2234	T2232	pobj	h,within
R1319	T2235	T2234	prep	of,h
R132	T387	T388	nmod	lung,ratio
R1320	T2236	T2235	pobj	birth,of
R1321	T2237	T2238	punct	[,16
R1322	T2238	T2231	parataxis	16,most
R1323	T2239	T2238	punct	],16
R1324	T2240	T2223	punct	.,died
R1325	T2242	T2243	prep	At,were
R1326	T2244	T2242	pobj	birth,At
R1327	T2245	T2243	punct	", ",were
R1328	T2246	T2243	nsubj	they,were
R1329	T2247	T2243	advmod	clearly,were
R133	T388	T386	pobj	ratio,in
R1330	T2248	T2243	acomp	smaller,were
R1331	T2249	T2248	prep	than,smaller
R1332	T2250	T2251	amod	wild,type
R1333	T2251	T2253	nmod	type,littermates
R1334	T2252	T2251	punct	-,type
R1335	T2253	T2249	pobj	littermates,than
R1336	T2254	T2251	cc	or,type
R1337	T2255	T2251	conj	heterozygous,type
R1338	T2256	T2243	punct	.,were
R1339	T2258	T2259	det	An,investigator
R134	T389	T387	punct	-,lung
R1340	T2259	T2261	nsubj	investigator,pick
R1341	T2260	T2259	amod	experienced,investigator
R1342	T2262	T2261	aux	can,pick
R1343	T2263	T2261	prt	out,pick
R1344	T2264	T2265	compound	knockout,pups
R1345	T2265	T2261	dobj	pups,pick
R1346	T2266	T2261	prep	based,pick
R1347	T2267	T2266	prep	on,based
R1348	T2268	T2267	pobj	this,on
R1349	T2269	T2261	prep	along,pick
R135	T390	T387	prep	to,lung
R1350	T2270	T2269	prep	with,along
R1351	T2271	T2270	pobj	presence,with
R1352	T2272	T2271	prep	of,presence
R1353	T2273	T2272	pobj	hypoxia,of
R1354	T2274	T2271	cc	and,presence
R1355	T2275	T2276	poss	their,lack
R1356	T2276	T2271	conj	lack,presence
R1357	T2277	T2276	prep	of,lack
R1358	T2278	T2279	amod	spontaneous,movement
R1359	T2279	T2277	pobj	movement,of
R136	T391	T390	punct	-,to
R1360	T2280	T2261	punct	.,pick
R1361	T2282	T2283	det	The,frequency
R1362	T2283	T2285	nsubj	frequency,was
R1363	T2284	T2283	compound	breathing,frequency
R1364	T2286	T2285	prep	in,was
R1365	T2287	T2288	nmod	P0,pups
R1366	T2288	T2286	pobj	pups,in
R1367	T2289	T2288	nmod	Dhcr7,pups
R1368	T2290	T2289	punct	-,Dhcr7
R1369	T2291	T2289	punct	/,Dhcr7
R137	T392	T393	compound	body,weight
R1370	T2292	T2289	punct	-,Dhcr7
R1371	T2293	T2294	advmod	markedly,slower
R1372	T2294	T2285	acomp	slower,was
R1373	T2295	T2294	prep	than,slower
R1374	T2296	T2297	compound	control,littermates
R1375	T2297	T2295	pobj	littermates,than
R1376	T2298	T2299	punct	(,breaths
R1377	T2299	T2285	parataxis	breaths,was
R1378	T2300	T2301	quantmod	34,7
R1379	T2301	T2299	nummod	7,breaths
R138	T393	T390	pobj	weight,to
R1380	T2302	T2301	punct	±,7
R1381	T2303	T2304	punct	/,min
R1382	T2304	T2299	prep	min,breaths
R1383	T2305	T2299	prep	in,breaths
R1384	T2306	T2305	pobj	Dhcr7,in
R1385	T2307	T2306	punct	-,Dhcr7
R1386	T2308	T2306	punct	/,Dhcr7
R1387	T2309	T2306	punct	-,Dhcr7
R1388	T2310	T2299	punct	", ",breaths
R1389	T2311	T2312	nsubj	n,breaths
R139	T394	T378	auxpass	was,noted
R1390	T2312	T2299	ccomp	breaths,breaths
R1391	T2313	T2312	punct	=,breaths
R1392	T2314	T2312	nummod	10,breaths
R1393	T2315	T2314	cc	vs.,10
R1394	T2316	T2317	quantmod	92,16
R1395	T2317	T2314	conj	16,10
R1396	T2318	T2317	punct	±,16
R1397	T2319	T2320	punct	/,min
R1398	T2320	T2312	prep	min,breaths
R1399	T2321	T2312	prep	in,breaths
R14	T252	T253	compound	Smith,Opitz
R140	T395	T378	prep	in,noted
R1400	T2322	T2321	pobj	controls,in
R1401	T2323	T2299	punct	", ",breaths
R1402	T2324	T2325	nsubj	n,32
R1403	T2325	T2299	ccomp	32,breaths
R1404	T2326	T2325	punct	=,32
R1405	T2327	T2299	punct	", ",breaths
R1406	T2328	T2329	nsubj	p,0.001
R1407	T2329	T2299	ccomp	0.001,breaths
R1408	T2330	T2329	punct	<,0.001
R1409	T2331	T2299	punct	),breaths
R141	T396	T397	nmod	Dhcr7,embryos
R1410	T2332	T2285	cc	and,was
R1411	T2333	T2334	det	the,breathing
R1412	T2334	T2335	nsubjpass	breathing,observed
R1413	T2335	T2285	conj	observed,was
R1414	T2336	T2335	auxpass	was,observed
R1415	T2337	T2338	aux	to,be
R1416	T2338	T2335	xcomp	be,observed
R1417	T2339	T2340	advmod	more,irregular
R1418	T2340	T2338	acomp	irregular,be
R1419	T2341	T2340	cc	and,irregular
R142	T397	T395	pobj	embryos,in
R1420	T2342	T2340	conj	shallow,irregular
R1421	T2343	T2338	punct	", ",be
R1422	T2344	T2338	prep	with,be
R1423	T2345	T2346	amod	long,periods
R1424	T2346	T2344	pobj	periods,with
R1425	T2347	T2346	prep	of,periods
R1426	T2348	T2347	pobj	apnea,of
R1427	T2349	T2335	punct	.,observed
R1428	T2351	T2352	aux	To,determine
R1429	T2352	T2353	advcl	determine,harvested
R143	T398	T396	punct	-,Dhcr7
R1430	T2354	T2355	det	the,stage
R1431	T2355	T2352	dobj	stage,determine
R1432	T2356	T2355	amod	gestational,stage
R1433	T2357	T2358	prep	at,affected
R1434	T2358	T2355	relcl	affected,stage
R1435	T2359	T2357	pobj	which,at
R1436	T2360	T2358	nsubjpass	growth,affected
R1437	T2361	T2360	prep	of,growth
R1438	T2362	T2363	det	the,embryos
R1439	T2363	T2361	pobj	embryos,of
R144	T399	T396	punct	/,Dhcr7
R1440	T2364	T2363	compound	knockout,embryos
R1441	T2365	T2358	auxpass	was,affected
R1442	T2366	T2353	punct	", ",harvested
R1443	T2367	T2353	nsubjpass	embryos,harvested
R1444	T2368	T2353	auxpass	were,harvested
R1445	T2369	T2353	prep	at,harvested
R1446	T2370	T2371	amod	various,stages
R1447	T2371	T2369	pobj	stages,at
R1448	T2372	T2371	prep	of,stages
R1449	T2373	T2372	pobj	gestation,of
R145	T400	T396	punct	-,Dhcr7
R1450	T2374	T2353	cc	and,harvested
R1451	T2375	T2376	poss	their,lengths
R1452	T2376	T2378	nsubj	lengths,compared
R1453	T2377	T2376	compound	body,lengths
R1454	T2378	T2353	conj	compared,harvested
R1455	T2379	T2378	prep	between,compared
R1456	T2380	T2379	pobj	Dhcr7,between
R1457	T2381	T2380	punct	-,Dhcr7
R1458	T2382	T2380	punct	/,Dhcr7
R1459	T2383	T2380	punct	-,Dhcr7
R146	T401	T378	punct	", ",noted
R1460	T2384	T2380	cc	and,Dhcr7
R1461	T2385	T2380	conj	controls,Dhcr7
R1462	T2386	T2353	punct	.,harvested
R1463	T2388	T2389	det	A,increase
R1464	T2389	T2391	nsubjpass	increase,observed
R1465	T2390	T2389	amod	linear,increase
R1466	T2392	T2389	prep	in,increase
R1467	T2393	T2394	compound	body,length
R1468	T2394	T2392	pobj	length,in
R1469	T2395	T2389	prep	from,increase
R147	T402	T378	prep	compared,noted
R1470	T2396	T2395	pobj	E9.5,from
R1471	T2397	T2395	prep	to,from
R1472	T2398	T2397	pobj	E19.5,to
R1473	T2399	T2389	prep	in,increase
R1474	T2400	T2401	compound	control,embryos
R1475	T2401	T2399	pobj	embryos,in
R1476	T2402	T2391	auxpass	was,observed
R1477	T2403	T2404	punct	(,Fig.
R1478	T2404	T2391	parataxis	Fig.,observed
R1479	T2405	T2404	nummod	1,Fig.
R148	T403	T402	prep	to,compared
R1480	T2406	T2404	punct	),Fig.
R1481	T2407	T2391	punct	.,observed
R1482	T2409	T2410	det	No,differences
R1483	T2410	T2412	nsubjpass	differences,noted
R1484	T2411	T2410	amod	significant,differences
R1485	T2413	T2410	prep	in,differences
R1486	T2414	T2415	compound	body,length
R1487	T2415	T2413	pobj	length,in
R1488	T2416	T2410	prep	between,differences
R1489	T2417	T2418	amod	wild,type
R149	T404	T403	pobj	controls,to
R1490	T2418	T2420	nmod	type,embryos
R1491	T2419	T2418	punct	-,type
R1492	T2420	T2416	pobj	embryos,between
R1493	T2421	T2418	cc	and,type
R1494	T2422	T2418	conj	heterozygous,type
R1495	T2423	T2412	auxpass	were,noted
R1496	T2424	T2425	punct	(,shown
R1497	T2425	T2412	parataxis	shown,noted
R1498	T2426	T2425	nsubj	data,shown
R1499	T2427	T2425	neg	not,shown
R15	T253	T251	compound	Opitz,syndrome
R150	T405	T378	punct	.,noted
R1500	T2428	T2425	punct	),shown
R1501	T2429	T2412	punct	.,noted
R1502	T2431	T2432	prep	In,showed
R1503	T2433	T2431	pobj	contrast,In
R1504	T2434	T2432	punct	", ",showed
R1505	T2435	T2436	compound	knockout,embryos
R1506	T2436	T2432	nsubj	embryos,showed
R1507	T2437	T2438	amod	comparable,growth
R1508	T2438	T2432	dobj	growth,showed
R1509	T2439	T2440	compound	body,length
R151	T407	T408	compound	Lung,branching
R1510	T2440	T2438	compound	length,growth
R1511	T2441	T2432	prep	until,showed
R1512	T2442	T2441	pobj	E14.5,until
R1513	T2443	T2444	punct	~,E16.5
R1514	T2444	T2442	prep	E16.5,E14.5
R1515	T2445	T2432	punct	", ",showed
R1516	T2446	T2447	advmod	then,began
R1517	T2447	T2432	dep	began,showed
R1518	T2448	T2449	aux	to,show
R1519	T2449	T2447	xcomp	show,began
R152	T408	T409	compound	branching,morphogenesis
R1520	T2450	T2451	det	a,increase
R1521	T2451	T2449	dobj	increase,show
R1522	T2452	T2451	amod	slower,increase
R1523	T2453	T2451	prep	in,increase
R1524	T2454	T2453	pobj	length,in
R1525	T2455	T2449	punct	", ",show
R1526	T2456	T2457	amod	such,was
R1527	T2457	T2449	advcl	was,show
R1528	T2458	T2457	mark	that,was
R1529	T2459	T2457	prep	by,was
R153	T409	T410	nsubj	morphogenesis,was
R1530	T2460	T2459	pobj	E17.5,by
R1531	T2461	T2457	punct	", ",was
R1532	T2462	T2457	nsubj	this,was
R1533	T2463	T2464	advmod	statistically,significant
R1534	T2464	T2457	acomp	significant,was
R1535	T2465	T2466	punct	(,Fig.
R1536	T2466	T2449	parataxis	Fig.,show
R1537	T2467	T2468	quantmod	18.87,1.29
R1538	T2468	T2470	nummod	1.29,mm
R1539	T2469	T2468	punct	±,1.29
R154	T411	T410	acomp	comparable,was
R1540	T2470	T2466	dep	mm,Fig.
R1541	T2471	T2470	prep	in,mm
R1542	T2472	T2471	pobj	controls,in
R1543	T2473	T2466	punct	", ",Fig.
R1544	T2474	T2475	nsubj	n,15
R1545	T2475	T2466	ccomp	15,Fig.
R1546	T2476	T2475	punct	=,15
R1547	T2477	T2475	prep	compared,15
R1548	T2478	T2477	prep	to,compared
R1549	T2479	T2480	quantmod	16.69,1.05
R155	T412	T410	prep	between,was
R1550	T2480	T2482	nummod	1.05,mm
R1551	T2481	T2480	punct	±,1.05
R1552	T2482	T2478	pobj	mm,to
R1553	T2483	T2482	prep	in,mm
R1554	T2484	T2483	pobj	Dhcr7,in
R1555	T2485	T2484	punct	-,Dhcr7
R1556	T2486	T2484	punct	/,Dhcr7
R1557	T2487	T2484	punct	-,Dhcr7
R1558	T2488	T2466	punct	", ",Fig.
R1559	T2489	T2490	nsubj	n,7
R156	T413	T412	pobj	Dhcr7,between
R1560	T2490	T2466	ccomp	7,Fig.
R1561	T2491	T2490	punct	=,7
R1562	T2492	T2466	punct	", ",Fig.
R1563	T2493	T2494	nsubj	p,0.05
R1564	T2494	T2466	ccomp	0.05,Fig.
R1565	T2495	T2494	punct	<,0.05
R1566	T2496	T2466	punct	", ",Fig.
R1567	T2497	T2466	nummod	1,Fig.
R1568	T2498	T2466	punct	),Fig.
R1569	T2499	T2432	punct	.,showed
R157	T414	T413	punct	-,Dhcr7
R1570	T2667	T2668	nmod	Dhcr7,embryos
R1571	T2668	T2672	nsubj	embryos,displayed
R1572	T2669	T2667	punct	-,Dhcr7
R1573	T2670	T2667	punct	/,Dhcr7
R1574	T2671	T2667	punct	-,Dhcr7
R1575	T2673	T2668	prep	at,embryos
R1576	T2674	T2675	amod	late,stages
R1577	T2675	T2673	pobj	stages,at
R1578	T2676	T2677	amod	pulmonary,hypoplasia
R1579	T2677	T2672	dobj	hypoplasia,displayed
R158	T415	T413	punct	/,Dhcr7
R1580	T2678	T2677	prep	with,hypoplasia
R1581	T2679	T2680	amod	delayed,development
R1582	T2680	T2678	pobj	development,with
R1583	T2681	T2680	amod	saccular,development
R1584	T2683	T2684	amod	Wet,lung
R1585	T2684	T2685	compound	lung,weights
R1586	T2685	T2686	nsubj	weights,were
R1587	T2687	T2685	punct	", ",weights
R1588	T2688	T2689	advmod	as,as
R1589	T2689	T2685	cc	as,weights
R159	T416	T413	punct	-,Dhcr7
R1590	T2690	T2689	advmod	well,as
R1591	T2691	T2692	nmod	lung,ratios
R1592	T2692	T2685	conj	ratios,weights
R1593	T2693	T2691	prep	to,lung
R1594	T2694	T2695	compound	body,weight
R1595	T2695	T2693	pobj	weight,to
R1596	T2696	T2697	advmod	significantly,lower
R1597	T2697	T2686	acomp	lower,were
R1598	T2698	T2686	prep	in,were
R1599	T2699	T2698	pobj	Dhcr7,in
R16	T254	T253	punct	-,Opitz
R160	T417	T413	cc	and,Dhcr7
R1600	T2700	T2699	punct	-,Dhcr7
R1601	T2701	T2699	punct	/,Dhcr7
R1602	T2702	T2699	punct	-,Dhcr7
R1603	T2703	T2686	prep	at,were
R1604	T2704	T2703	pobj	E18.5,at
R1605	T2705	T2686	prep	compared,were
R1606	T2706	T2705	prep	to,compared
R1607	T2707	T2706	pobj	those,to
R1608	T2708	T2707	prep	in,those
R1609	T2709	T2710	amod	wild,type
R161	T418	T413	conj	controls,Dhcr7
R1610	T2710	T2708	pobj	type,in
R1611	T2711	T2710	punct	-,type
R1612	T2712	T2686	cc	but,were
R1613	T2713	T2712	neg	not,but
R1614	T2714	T2712	prep	at,but
R1615	T2715	T2714	pobj	E13.5,at
R1616	T2716	T2717	punct	(,Table
R1617	T2717	T2686	parataxis	Table,were
R1618	T2718	T2717	nummod	1,Table
R1619	T2719	T2717	punct	),Table
R162	T419	T410	prep	at,was
R1620	T2720	T2686	punct	.,were
R1621	T2722	T2723	preconj	Both,body
R1622	T2723	T2724	nmod	body,weights
R1623	T2724	T2727	nsubjpass	weights,reduced
R1624	T2725	T2723	cc	and,body
R1625	T2726	T2723	conj	lung,body
R1626	T2728	T2727	auxpass	were,reduced
R1627	T2729	T2727	punct	", ",reduced
R1628	T2730	T2727	cc	but,reduced
R1629	T2731	T2732	nsubj	lungs,seemed
R163	T420	T421	amod	early,stages
R1630	T2732	T2727	conj	seemed,reduced
R1631	T2733	T2734	aux	to,be
R1632	T2734	T2732	xcomp	be,seemed
R1633	T2735	T2736	advmod	more,affected
R1634	T2736	T2734	acomp	affected,be
R1635	T2737	T2732	punct	.,seemed
R1636	T2739	T2740	det	A,figure
R1637	T2740	T2742	nsubjpass	figure,shown
R1638	T2741	T2740	amod	representative,figure
R1639	T2743	T2740	prep	of,figure
R164	T421	T419	pobj	stages,at
R1640	T2744	T2743	pobj	embryos,of
R1641	T2745	T2744	prep	at,embryos
R1642	T2746	T2745	pobj	E10.5,at
R1643	T2747	T2743	cc	and,of
R1644	T2748	T2743	conj	of,of
R1645	T2749	T2750	det	the,pups
R1646	T2750	T2748	pobj	pups,of
R1647	T2751	T2750	prep	at,pups
R1648	T2752	T2751	pobj	birth,at
R1649	T2753	T2742	auxpass	was,shown
R165	T422	T410	punct	", ",was
R1650	T2754	T2742	prep	in,shown
R1651	T2755	T2756	nmod	Fig.,2A
R1652	T2756	T2754	pobj	2A,in
R1653	T2757	T2756	cc	and,2A
R1654	T2758	T2756	conj	2B,2A
R1655	T2759	T2742	punct	.,shown
R1656	T2778	T2773	cc	u,e
R1657	T2779	T2780	nsubj	ngs,r
R1658	T2780	T2773	conj	r,e
R1659	T2781	T2779	prep	fo,ngs
R166	T423	T410	cc	but,was
R1660	T2782	T2780	acomp	a,r
R1661	T2783	T2782	prep	n,a
R1662	T2786	T2787	amod	g,r
R1663	T2790	T2789	pobj	abnormalities,o
R1664	T2791	T2790	amod	ss,abnormalities
R1665	T2792	T2790	amod	morphological,abnormalities
R1666	T2795	T2796	det	The,organogenesis
R1667	T2796	T2798	nsubjpass	organogenesis,preserved
R1668	T2797	T2796	amod	overall,organogenesis
R1669	T2798	T2802	ccomp	preserved,seen
R167	T424	T425	amod	delayed,development
R1670	T2799	T2796	prep	of,organogenesis
R1671	T2800	T2799	pobj	lungs,of
R1672	T2801	T2798	auxpass	was,preserved
R1673	T2803	T2798	prep	in,preserved
R1674	T2804	T2805	nmod	Dhcr7,pups
R1675	T2805	T2803	pobj	pups,in
R1676	T2806	T2804	punct	-,Dhcr7
R1677	T2807	T2804	punct	/,Dhcr7
R1678	T2808	T2804	punct	-,Dhcr7
R1679	T2809	T2802	punct	;,seen
R168	T425	T427	nsubj	development,was
R1680	T2810	T2811	nummod	four,lobes
R1681	T2811	T2802	nsubjpass	lobes,seen
R1682	T2812	T2811	amod	right,lobes
R1683	T2813	T2811	compound	lung,lobes
R1684	T2814	T2811	cc	and,lobes
R1685	T2815	T2816	det	a,lobe
R1686	T2816	T2811	conj	lobe,lobes
R1687	T2817	T2816	amod	single,lobe
R1688	T2818	T2816	amod	left,lobe
R1689	T2819	T2816	acl	flanking,lobe
R169	T426	T425	amod	saccular,development
R1690	T2820	T2821	det	the,heart
R1691	T2821	T2819	dobj	heart,flanking
R1692	T2822	T2802	auxpass	were,seen
R1693	T2823	T2802	advmod	easily,seen
R1694	T2824	T2802	prep	on,seen
R1695	T2825	T2826	amod	external,examination
R1696	T2826	T2824	pobj	examination,on
R1697	T2827	T2802	prep	at,seen
R1698	T2828	T2827	pobj	birth,at
R1699	T2829	T2830	punct	(,Fig.
R17	T255	T253	compound	Lemli,Opitz
R170	T427	T410	conj	was,was
R1700	T2830	T2802	parataxis	Fig.,seen
R1701	T2831	T2830	nummod	2D,Fig.
R1702	T2832	T2830	punct	),Fig.
R1703	T2833	T2802	punct	.,seen
R1704	T2835	T2836	nsubj	Examination,showed
R1705	T2837	T2835	prep	for,Examination
R1706	T2838	T2837	pobj	lobation,for
R1707	T2839	T2835	prep	at,Examination
R1708	T2840	T2839	pobj	E13.5,at
R1709	T2841	T2836	advmod	also,showed
R171	T428	T427	acomp	visible,was
R1710	T2842	T2843	amod	comparable,anatomy
R1711	T2843	T2836	dobj	anatomy,showed
R1712	T2844	T2843	amod	gross,anatomy
R1713	T2845	T2846	punct	(,Fig.
R1714	T2846	T2836	parataxis	Fig.,showed
R1715	T2847	T2846	nummod	2C,Fig.
R1716	T2848	T2846	punct	),Fig.
R1717	T2849	T2836	prep	between,showed
R1718	T2850	T2851	amod	wild,type
R1719	T2851	T2853	nmod	type,embryos
R172	T429	T427	prep	in,was
R1720	T2852	T2851	punct	-,type
R1721	T2853	T2849	pobj	embryos,between
R1722	T2854	T2851	cc	and,type
R1723	T2855	T2851	conj	knockout,type
R1724	T2856	T2836	punct	.,showed
R1725	T2858	T2859	amod	Histological,analyses
R1726	T2859	T2860	nsubj	analyses,confirmed
R1727	T2861	T2859	prep	of,analyses
R1728	T2862	T2863	det	the,structures
R1729	T2863	T2861	pobj	structures,of
R173	T430	T431	det	all,embryos
R1730	T2864	T2863	amod	cardiopulmonary,structures
R1731	T2865	T2859	prep	at,analyses
R1732	T2866	T2865	pobj	E20.5,at
R1733	T2867	T2859	punct	", ",analyses
R1734	T2868	T2859	acl	taken,analyses
R1735	T2869	T2870	advmod	just,before
R1736	T2870	T2868	prep	before,taken
R1737	T2871	T2870	pobj	birth,before
R1738	T2872	T2873	aux	to,avoid
R1739	T2873	T2868	advcl	avoid,taken
R174	T431	T429	pobj	embryos,in
R1740	T2874	T2873	dobj	artifacts,avoid
R1741	T2875	T2873	prep	from,avoid
R1742	T2876	T2875	pobj	lack,from
R1743	T2877	T2876	prep	of,lack
R1744	T2878	T2879	compound	lung,inflation
R1745	T2879	T2877	pobj	inflation,of
R1746	T2880	T2860	punct	", ",confirmed
R1747	T2881	T2860	advmod	also,confirmed
R1748	T2882	T2883	amod	normal,development
R1749	T2883	T2860	dobj	development,confirmed
R175	T432	T431	nmod	Dhcr7,embryos
R1750	T2884	T2883	amod	gross,development
R1751	T2885	T2883	amod	anatomic,development
R1752	T2886	T2887	punct	(,2E
R1753	T2887	T2860	parataxis	2E,confirmed
R1754	T2888	T2887	dep	Fig.,2E
R1755	T2889	T2887	cc	and,2E
R1756	T2890	T2887	conj	2F,2E
R1757	T2891	T2887	punct	),2E
R1758	T2892	T2860	punct	.,confirmed
R1759	T2894	T2895	advmod	Additionally,identified
R176	T433	T432	punct	-,Dhcr7
R1760	T2896	T2895	punct	", ",identified
R1761	T2897	T2898	det	no,defects
R1762	T2898	T2895	nsubjpass	defects,identified
R1763	T2899	T2898	amod	cardiac,defects
R1764	T2900	T2895	auxpass	were,identified
R1765	T2901	T2895	cc	and,identified
R1766	T2902	T2903	nsubjpass	this,confirmed
R1767	T2903	T2895	conj	confirmed,identified
R1768	T2904	T2903	auxpass	was,confirmed
R1769	T2905	T2903	agent	by,confirmed
R177	T434	T432	punct	/,Dhcr7
R1770	T2906	T2907	advmod	more,detailed
R1771	T2907	T2908	amod	detailed,analyses
R1772	T2908	T2905	pobj	analyses,by
R1773	T2909	T2908	amod	histological,analyses
R1774	T2910	T2911	punct	(,shown
R1775	T2911	T2903	parataxis	shown,confirmed
R1776	T2912	T2911	nsubj	data,shown
R1777	T2913	T2911	neg	not,shown
R1778	T2914	T2911	punct	),shown
R1779	T2915	T2895	punct	.,identified
R178	T435	T432	punct	-,Dhcr7
R1780	T2917	T2918	advmod	Thus,observed
R1781	T2919	T2918	punct	", ",observed
R1782	T2920	T2918	prep	despite,observed
R1783	T2921	T2922	det	the,hypoxia
R1784	T2922	T2920	pobj	hypoxia,despite
R1785	T2923	T2922	amod	obvious,hypoxia
R1786	T2924	T2925	advmod	frequently,observed
R1787	T2925	T2922	acl	observed,hypoxia
R1788	T2926	T2925	prep	at,observed
R1789	T2927	T2926	pobj	birth,at
R179	T436	T437	prep	from,onwards
R1790	T2928	T2927	prep	of,birth
R1791	T2929	T2930	det	the,pups
R1792	T2930	T2928	pobj	pups,of
R1793	T2931	T2930	compound	knockout,pups
R1794	T2932	T2918	punct	", ",observed
R1795	T2933	T2934	amod	major,abnormalities
R1796	T2934	T2918	nsubjpass	abnormalities,observed
R1797	T2935	T2934	amod	cardiac,abnormalities
R1798	T2936	T2934	amod	structural,abnormalities
R1799	T2937	T2918	auxpass	were,observed
R18	T256	T253	punct	-,Opitz
R180	T437	T427	advmod	onwards,was
R1800	T2938	T2918	neg	not,observed
R1801	T2939	T2940	aux	to,account
R1802	T2940	T2918	xcomp	account,observed
R1803	T2941	T2940	prep	for,account
R1804	T2942	T2943	det	this,phenotype
R1805	T2943	T2941	pobj	phenotype,for
R1806	T2944	T2918	punct	.,observed
R1807	T2946	T2947	det	No,differences
R1808	T2947	T2950	nsubj	differences,were
R1809	T2948	T2947	amod	distinguishable,differences
R181	T438	T436	pobj	E17.5,from
R1810	T2949	T2947	amod	morphological,differences
R1811	T2951	T2950	acomp	discernable,were
R1812	T2952	T2950	prep	between,were
R1813	T2953	T2954	nmod	Dhcr7,lungs
R1814	T2954	T2952	pobj	lungs,between
R1815	T2955	T2953	punct	-,Dhcr7
R1816	T2956	T2953	punct	/,Dhcr7
R1817	T2957	T2953	punct	-,Dhcr7
R1818	T2958	T2953	cc	and,Dhcr7
R1819	T2959	T2960	amod	wild,type
R182	T439	T427	punct	.,was
R1820	T2960	T2953	conj	type,Dhcr7
R1821	T2961	T2960	punct	-,type
R1822	T2962	T2950	prep	at,were
R1823	T2963	T2962	pobj	E10.5,at
R1824	T2964	T2963	punct	", ",E10.5
R1825	T2965	T2963	conj	E13.5,E10.5
R1826	T2966	T2965	punct	", ",E13.5
R1827	T2967	T2965	conj	E14.5,E13.5
R1828	T2968	T2967	cc	and,E14.5
R1829	T2969	T2967	conj	E16.5,E14.5
R183	T441	T442	amod	Impaired,development
R1830	T2970	T2950	prep	at,were
R1831	T2971	T2972	amod	low,magnifications
R1832	T2972	T2970	pobj	magnifications,at
R1833	T2973	T2974	punct	(,10X
R1834	T2974	T2971	parataxis	10X,low
R1835	T2975	T2974	punct	),10X
R1836	T2976	T2971	cc	or,low
R1837	T2977	T2971	conj	high,low
R1838	T2978	T2979	punct	(,40X
R1839	T2979	T2977	parataxis	40X,high
R184	T442	T444	nsubj	development,were
R1840	T2980	T2979	punct	),40X
R1841	T2981	T2982	punct	(,A
R1842	T2982	T2950	parataxis	A,were
R1843	T2983	T2982	dep	Fig.,A
R1844	T2984	T2983	nummod	3,Fig.
R1845	T2985	T2982	punct	", ",A
R1846	T2986	T2982	compound	panel,A
R1847	T2987	T2982	punct	: ,A
R1848	T2988	T2982	meta	a,A
R1849	T2989	T2988	punct	-,a
R185	T443	T442	amod	pre-alveolar,development
R1850	T2990	T2988	dep	h,a
R1851	T2991	T2982	punct	", ",A
R1852	T2992	T2993	nsubj	data,shown
R1853	T2993	T2982	ccomp	shown,A
R1854	T2994	T2992	prep	for,data
R1855	T2995	T2994	pobj	E10.5,for
R1856	T2996	T2995	cc	and,E10.5
R1857	T2997	T2995	conj	E13.5,E10.5
R1858	T2998	T2993	neg	not,shown
R1859	T2999	T2982	punct	),A
R186	T445	T442	prep	of,development
R1860	T3000	T2950	punct	.,were
R1861	T3002	T3003	advmod	However,appeared
R1862	T3004	T3003	punct	", ",appeared
R1863	T3005	T3003	prep	from,appeared
R1864	T3006	T3005	pobj	E17.5,from
R1865	T3007	T3006	advmod	onwards,E17.5
R1866	T3008	T3003	punct	", ",appeared
R1867	T3009	T3003	nsubj	lungs,appeared
R1868	T3010	T3009	prep	from,lungs
R1869	T3011	T3012	compound	knockout,embryos
R187	T446	T447	amod	varying,severity
R1870	T3012	T3010	pobj	embryos,from
R1871	T3013	T3014	aux	to,have
R1872	T3014	T3003	xcomp	have,appeared
R1873	T3015	T3016	advmod	less,space
R1874	T3016	T3014	dobj	space,have
R1875	T3017	T3016	amod	saccular,space
R1876	T3018	T3016	cc	and,space
R1877	T3019	T3020	advmod	relatively,more
R1878	T3020	T3021	amod	more,mesenchyme
R1879	T3021	T3016	conj	mesenchyme,space
R188	T447	T445	pobj	severity,of
R1880	T3022	T3014	prep	compared,have
R1881	T3023	T3022	prep	to,compared
R1882	T3024	T3025	amod	wild,type
R1883	T3025	T3027	compound	type,lungs
R1884	T3026	T3025	punct	-,type
R1885	T3027	T3023	pobj	lungs,to
R1886	T3028	T3029	punct	(,A
R1887	T3029	T3014	parataxis	A,have
R1888	T3030	T3029	dep	Fig.,A
R1889	T3031	T3030	nummod	3,Fig.
R189	T448	T442	punct	", ",development
R1890	T3032	T3029	punct	", ",A
R1891	T3033	T3029	compound	panel,A
R1892	T3034	T3029	punct	: ,A
R1893	T3035	T3029	meta	I,A
R1894	T3036	T3035	punct	-,I
R1895	T3037	T3035	dep	x,I
R1896	T3038	T3029	punct	),A
R1897	T3039	T3003	punct	.,appeared
R1898	T3041	T3042	nsubj	Lungs,showed
R1899	T3043	T3041	prep	from,Lungs
R19	T261	T262	amod	Normal,biosynthesis
R190	T449	T450	amod	inhibited,proliferation
R1900	T3044	T3045	compound	control,animals
R1901	T3045	T3043	pobj	animals,from
R1902	T3046	T3047	amod	normal,progression
R1903	T3047	T3042	dobj	progression,showed
R1904	T3048	T3047	prep	with,progression
R1905	T3049	T3048	pobj	formation,with
R1906	T3050	T3049	prep	of,formation
R1907	T3051	T3052	npadvmod	sac,like
R1908	T3052	T3054	amod	like,structures
R1909	T3053	T3052	punct	-,like
R191	T450	T442	conj	proliferation,development
R1910	T3054	T3050	pobj	structures,of
R1911	T3055	T3054	punct	", ",structures
R1912	T3056	T3057	det	the,precursors
R1913	T3057	T3054	appos	precursors,structures
R1914	T3058	T3057	prep	of,precursors
R1915	T3059	T3060	det	the,alveoli
R1916	T3060	T3058	pobj	alveoli,of
R1917	T3061	T3062	punct	(,A
R1918	T3062	T3042	parataxis	A,showed
R1919	T3063	T3062	dep	Fig.,A
R192	T451	T450	compound	cell,proliferation
R1920	T3064	T3063	nummod	3,Fig.
R1921	T3065	T3062	punct	", ",A
R1922	T3066	T3062	compound	panel,A
R1923	T3067	T3062	punct	: ,A
R1924	T3068	T3062	meta	i,A
R1925	T3069	T3068	punct	", ",i
R1926	T3070	T3068	conj	m,i
R1927	T3071	T3070	punct	", ",m
R1928	T3072	T3070	conj	q,m
R1929	T3073	T3072	cc	and,q
R193	T452	T450	punct	", ",proliferation
R1930	T3074	T3072	conj	u,q
R1931	T3075	T3062	punct	),A
R1932	T3076	T3042	punct	.,showed
R1933	T3078	T3079	prep	In,occurred
R1934	T3080	T3078	pobj	lungs,In
R1935	T3081	T3080	prep	from,lungs
R1936	T3082	T3083	compound	knockout,embryos
R1937	T3083	T3081	pobj	embryos,from
R1938	T3084	T3079	punct	", ",occurred
R1939	T3085	T3079	nsubj	expansion,occurred
R194	T453	T454	amod	delayed,differentiation
R1940	T3086	T3085	prep	of,expansion
R1941	T3087	T3088	amod	epithelial,tubules
R1942	T3088	T3086	pobj	tubules,of
R1943	T3089	T3079	cc	but,occurred
R1944	T3090	T3091	auxpass	was,delayed
R1945	T3091	T3079	conj	delayed,occurred
R1946	T3092	T3091	advmod	significantly,delayed
R1947	T3093	T3094	punct	(,v
R1948	T3094	T3091	parataxis	v,delayed
R1949	T3095	T3094	dep	Fig.,v
R195	T454	T450	conj	differentiation,proliferation
R1950	T3096	T3095	nummod	3,Fig.
R1951	T3097	T3094	punct	", ",v
R1952	T3098	T3099	advmod	cf.,A
R1953	T3099	T3094	dep	A,v
R1954	T3100	T3099	compound	panel,A
R1955	T3101	T3094	punct	: ,v
R1956	T3102	T3094	nmod	j,v
R1957	T3103	T3094	punct	", ",v
R1958	T3104	T3094	nmod	n,v
R1959	T3105	T3094	punct	", ",v
R196	T455	T454	prep	of,differentiation
R1960	T3106	T3094	nmod	r,v
R1961	T3107	T3094	punct	", ",v
R1962	T3108	T3094	prep	with,v
R1963	T3109	T3108	pobj	i,with
R1964	T3110	T3109	punct	", ",i
R1965	T3111	T3109	conj	m,i
R1966	T3112	T3111	punct	", ",m
R1967	T3113	T3111	conj	q,m
R1968	T3114	T3113	cc	and,q
R1969	T3115	T3113	conj	u,q
R197	T456	T455	pobj	type,of
R1970	T3116	T3094	punct	),v
R1971	T3117	T3079	punct	.,occurred
R1972	T3119	T3120	amod	Morphometric,measurements
R1973	T3120	T3121	nsubj	measurements,showed
R1974	T3122	T3120	prep	of,measurements
R1975	T3123	T3124	compound	lung,sections
R1976	T3124	T3122	pobj	sections,of
R1977	T3125	T3120	prep	at,measurements
R1978	T3126	T3127	nmod	E17.5,E20.5
R1979	T3127	T3125	pobj	E20.5,at
R198	T457	T456	nummod	I,type
R1980	T3128	T3127	punct	", ",E20.5
R1981	T3129	T3127	nmod	E18.5,E20.5
R1982	T3130	T3127	punct	", ",E20.5
R1983	T3131	T3120	cc	and,measurements
R1984	T3132	T3120	conj	in,measurements
R1985	T3133	T3132	pobj	lungs,in
R1986	T3134	T3133	prep	from,lungs
R1987	T3135	T3134	pobj	neonates,from
R1988	T3136	T3137	mark	that,decreased
R1989	T3137	T3121	ccomp	decreased,showed
R199	T458	T459	amod	alveolar,cells
R1990	T3138	T3139	det	the,proportion
R1991	T3139	T3137	nsubjpass	proportion,decreased
R1992	T3140	T3139	prep	of,proportion
R1993	T3141	T3142	amod	terminal,space
R1994	T3142	T3144	compound	space,area
R1995	T3143	T3142	compound	sac,space
R1996	T3144	T3140	pobj	area,of
R1997	T3145	T3137	auxpass	was,decreased
R1998	T3146	T3137	advmod	significantly,decreased
R1999	T3147	T3137	prep	in,decreased
R20	T262	T265	nsubj	biosynthesis,is
R200	T459	T456	appos	cells,type
R2000	T3148	T3149	nmod	Dhcr7,lungs
R2001	T3149	T3147	pobj	lungs,in
R2002	T3150	T3148	punct	-,Dhcr7
R2003	T3151	T3148	punct	/,Dhcr7
R2004	T3152	T3148	punct	-,Dhcr7
R2005	T3153	T3137	punct	", ",decreased
R2006	T3154	T3137	prep	compared,decreased
R2007	T3155	T3156	amod	wild,type
R2008	T3156	T3158	compound	type,lungs
R2009	T3157	T3156	punct	-,type
R201	T460	T459	amod	epithelial,cells
R2010	T3158	T3154	pobj	lungs,compared
R2011	T3159	T3160	punct	(,B
R2012	T3160	T3137	parataxis	B,decreased
R2013	T3161	T3160	dep	Fig.,B
R2014	T3162	T3161	nummod	3,Fig.
R2015	T3163	T3160	punct	", ",B
R2016	T3164	T3160	compound	panel,B
R2017	T3165	T3160	punct	),B
R2018	T3166	T3121	punct	.,showed
R2019	T3168	T3169	amod	Interstitial,mesenchyme
R202	T461	T459	punct	(,cells
R2020	T3169	T3170	nsubj	mesenchyme,appeared
R2021	T3171	T3170	oprd	thicker,appeared
R2022	T3172	T3170	cc	and,appeared
R2023	T3173	T3174	det	the,lungs
R2024	T3174	T3175	nsubj	lungs,had
R2025	T3175	T3170	conj	had,appeared
R2026	T3176	T3177	amod	less,septation
R2027	T3177	T3175	dobj	septation,had
R2028	T3178	T3175	prep	compared,had
R2029	T3179	T3178	prep	to,compared
R203	T462	T459	appos	AECs,cells
R2030	T3180	T3181	compound	control,lungs
R2031	T3181	T3179	pobj	lungs,to
R2032	T3182	T3175	punct	.,had
R2033	T3184	T3185	det	This,thickening
R2034	T3185	T3187	nsubj	thickening,explain
R2035	T3186	T3185	punct	',thickening
R2036	T3188	T3185	punct	',thickening
R2037	T3189	T3185	prep	of,thickening
R2038	T3190	T3191	det	the,alveoli
R2039	T3191	T3189	pobj	alveoli,of
R204	T463	T459	punct	),cells
R2040	T3192	T3187	aux	may,explain
R2041	T3193	T3194	det	the,hypoxia
R2042	T3194	T3187	dobj	hypoxia,explain
R2043	T3195	T3196	advmod	frequently,seen
R2044	T3196	T3194	acl	seen,hypoxia
R2045	T3197	T3196	prep	in,seen
R2046	T3198	T3199	nmod	Dhcr7,pups
R2047	T3199	T3197	pobj	pups,in
R2048	T3200	T3198	punct	-,Dhcr7
R2049	T3201	T3198	punct	/,Dhcr7
R205	T464	T454	cc	and,differentiation
R2050	T3202	T3198	punct	-,Dhcr7
R2051	T3203	T3196	prep	at,seen
R2052	T3204	T3203	pobj	birth,at
R2053	T3205	T3187	punct	.,explain
R206	T465	T466	amod	delayed,development
R2060	T3338	T3339	amod	Attenuated,proliferation
R2061	T3340	T3339	compound	cell,proliferation
R2062	T3341	T3339	prep	in,proliferation
R2063	T3342	T3343	amod	late,gestational
R2064	T3343	T3344	amod	gestational,lungs
R2065	T3344	T3341	pobj	lungs,in
R2066	T3345	T3344	nmod	Dhcr7,lungs
R2067	T3346	T3345	punct	-,Dhcr7
R2068	T3347	T3345	punct	/,Dhcr7
R2069	T3348	T3345	punct	-,Dhcr7
R207	T466	T454	conj	development,differentiation
R2070	T3350	T3351	aux	Does,lead
R2071	T3352	T3353	det	the,loss
R2072	T3353	T3351	nsubj	loss,lead
R2073	T3354	T3353	prep	of,loss
R2074	T3355	T3356	amod	normal,biosynthesis
R2075	T3356	T3354	pobj	biosynthesis,of
R2076	T3357	T3356	compound	cholesterol,biosynthesis
R2077	T3358	T3351	prep	to,lead
R2078	T3359	T3358	pobj	abnormalities,to
R2079	T3360	T3359	prep	of,abnormalities
R208	T467	T466	amod	vascular,development
R2080	T3361	T3362	nmod	cell,division
R2081	T3362	T3360	pobj	division,of
R2082	T3363	T3362	nmod	proliferation,division
R2083	T3364	T3362	punct	/,division
R2084	T3365	T3359	cc	or,abnormalities
R2085	T3366	T3367	compound	cell,death
R2086	T3367	T3359	conj	death,abnormalities
R2087	T3368	T3351	punct	?,lead
R2088	T3370	T3371	compound	Cell,proliferation
R2089	T3371	T3372	nsubjpass	proliferation,examined
R209	T468	T444	advmod	all,were
R2090	T3373	T3372	auxpass	was,examined
R2091	T3374	T3372	prep	by,examined
R2092	T3375	T3374	pobj	immunostaining,by
R2093	T3376	T3375	prep	for,immunostaining
R2094	T3377	T3376	pobj	PCNA,for
R2095	T3378	T3374	cc	and,by
R2096	T3379	T3380	advmod	also,by
R2097	T3380	T3374	conj	by,by
R2098	T3381	T3382	compound	BrdU,incorporation
R2099	T3382	T3380	pobj	incorporation,by
R21	T263	T262	amod	post-squalene,biosynthesis
R210	T469	T444	acomp	evident,were
R2100	T3383	T3372	punct	.,examined
R2101	T3385	T3386	preconj	Both,PCNA
R2102	T3386	T3387	nmod	PCNA,staining
R2103	T3387	T3390	nsubj	staining,showed
R2104	T3388	T3386	cc	and,PCNA
R2105	T3389	T3386	conj	BrdU,PCNA
R2106	T3391	T3387	prep	of,staining
R2107	T3392	T3393	amod	wild,type
R2108	T3393	T3395	nmod	type,lungs
R2109	T3394	T3393	punct	-,type
R211	T470	T444	prep	in,were
R2110	T3395	T3391	pobj	lungs,of
R2111	T3396	T3393	cc	and,type
R2112	T3397	T3393	conj	knockout,type
R2113	T3398	T3387	prep	at,staining
R2114	T3399	T3400	amod	early,stages
R2115	T3400	T3398	pobj	stages,at
R2116	T3401	T3400	amod	gestational,stages
R2117	T3402	T3400	punct	(,stages
R2118	T3403	T3400	appos	E13.5,stages
R2119	T3404	T3403	cc	and,E13.5
R212	T471	T472	compound	knockout,lungs
R2120	T3405	T3403	conj	E14.5,E13.5
R2121	T3406	T3400	punct	),stages
R2122	T3407	T3408	amod	comparable,patterns
R2123	T3408	T3390	dobj	patterns,showed
R2124	T3409	T3410	punct	(,A
R2125	T3410	T3390	parataxis	A,showed
R2126	T3411	T3410	dep	Fig.,A
R2127	T3412	T3411	nummod	4,Fig.
R2128	T3413	T3410	punct	", ",A
R2129	T3414	T3410	compound	panels,A
R213	T472	T470	pobj	lungs,in
R2130	T3415	T3416	punct	-,D
R2131	T3416	T3410	prep	D,A
R2132	T3417	T3410	punct	),A
R2133	T3418	T3390	punct	.,showed
R2134	T3420	T3421	advmod	However,visualized
R2135	T3422	T3421	punct	", ",visualized
R2136	T3423	T3424	det	a,reduction
R2137	T3424	T3421	nsubjpass	reduction,visualized
R2138	T3425	T3424	amod	clear,reduction
R2139	T3426	T3424	prep	of,reduction
R214	T473	T444	punct	.,were
R2140	T3427	T3428	nmod	PCNA,labeling
R2141	T3428	T3426	pobj	labeling,of
R2142	T3429	T3427	cc	and,PCNA
R2143	T3430	T3427	conj	BrdU,PCNA
R2144	T3431	T3421	auxpass	was,visualized
R2145	T3432	T3421	prep	in,visualized
R2146	T3433	T3434	amod	distal,lungs
R2147	T3434	T3432	pobj	lungs,in
R2148	T3435	T3434	prep	of,lungs
R2149	T3436	T3437	compound	knockout,embryos
R215	T475	T476	nsubj	Differentiation,was
R2150	T3437	T3435	pobj	embryos,of
R2151	T3438	T3421	prep	at,visualized
R2152	T3439	T3440	amod	late,stages
R2153	T3440	T3438	pobj	stages,at
R2154	T3441	T3440	amod	gestational,stages
R2155	T3442	T3443	punct	(,E
R2156	T3443	T3421	parataxis	E,visualized
R2157	T3444	T3443	npadvmod	E18.5,E
R2158	T3445	T3444	cc	and,E18.5
R2159	T3446	T3444	conj	E20.5,E18.5
R216	T477	T475	prep	of,Differentiation
R2160	T3447	T3443	punct	", ",E
R2161	T3448	T3443	dep	Fig.,E
R2162	T3449	T3448	nummod	4,Fig.
R2163	T3450	T3443	punct	", ",E
R2164	T3451	T3443	compound	panels,E
R2165	T3452	T3453	punct	-,H
R2166	T3453	T3443	prep	H,E
R2167	T3454	T3443	punct	),E
R2168	T3455	T3421	punct	.,visualized
R2169	T3457	T3458	amod	Attenuated,labeling
R217	T478	T479	nmod	type,AECs
R2170	T3458	T3460	nsubjpass	labeling,noted
R2171	T3459	T3458	compound	PCNA,labeling
R2172	T3461	T3460	auxpass	was,noted
R2173	T3462	T3460	advmod	also,noted
R2174	T3463	T3460	prep	in,noted
R2175	T3464	T3465	compound	knockout,hearts
R2176	T3465	T3463	pobj	hearts,in
R2177	T3466	T3460	prep	at,noted
R2178	T3467	T3466	pobj	term,at
R2179	T3468	T3469	punct	(,I
R218	T479	T477	pobj	AECs,of
R2180	T3469	T3460	parataxis	I,noted
R2181	T3470	T3469	dep	Fig.,I
R2182	T3471	T3470	nummod	4,Fig.
R2183	T3472	T3469	punct	", ",I
R2184	T3473	T3469	advmod	cf.,I
R2185	T3474	T3469	compound	panels,I
R2186	T3475	T3469	cc	and,I
R2187	T3476	T3469	conj	J,I
R2188	T3477	T3469	punct	),I
R2189	T3478	T3460	punct	.,noted
R219	T480	T478	nummod	II,type
R2190	T3480	T3481	advmod	When,stained
R2191	T3481	T3484	advcl	stained,observed
R2192	T3482	T3481	nsubjpass	lungs,stained
R2193	T3483	T3481	auxpass	were,stained
R2194	T3485	T3481	prep	for,stained
R2195	T3486	T3485	pobj	pHH3,for
R2196	T3487	T3486	punct	", ",pHH3
R2197	T3488	T3489	det	a,marker
R2198	T3489	T3486	appos	marker,pHH3
R2199	T3490	T3489	prep	of,marker
R22	T264	T262	compound	cholesterol,biosynthesis
R220	T481	T482	advmod	apparently,normal
R2200	T3491	T3492	amod	active,division
R2201	T3492	T3490	pobj	division,of
R2202	T3493	T3492	compound	cell,division
R2203	T3494	T3484	punct	", ",observed
R2204	T3495	T3496	det	a,reduction
R2205	T3496	T3484	nsubjpass	reduction,observed
R2206	T3497	T3496	amod	clear,reduction
R2207	T3498	T3496	prep	of,reduction
R2208	T3499	T3500	compound	pHH3,expression
R2209	T3500	T3498	pobj	expression,of
R221	T482	T476	acomp	normal,was
R2210	T3501	T3496	prep	in,reduction
R2211	T3502	T3503	det	the,epithelium
R2212	T3503	T3501	pobj	epithelium,in
R2213	T3504	T3503	prep	of,epithelium
R2214	T3505	T3506	compound	knockout,lung
R2215	T3506	T3504	pobj	lung,of
R2216	T3507	T3484	auxpass	was,observed
R2217	T3508	T3484	prep	at,observed
R2218	T3509	T3508	pobj	E20.5,at
R2219	T3510	T3508	cc	but,at
R222	T483	T484	mark	as,judged
R2220	T3511	T3510	neg	not,but
R2221	T3512	T3508	conj	at,at
R2222	T3513	T3512	pobj	E14.5,at
R2223	T3514	T3515	punct	(,K
R2224	T3515	T3484	parataxis	K,observed
R2225	T3516	T3515	dep	Fig.,K
R2226	T3517	T3516	nummod	4,Fig.
R2227	T3518	T3515	punct	", ",K
R2228	T3519	T3515	compound	panels,K
R2229	T3520	T3515	punct	", ",K
R223	T484	T476	advcl	judged,was
R2230	T3521	T3515	conj	L,K
R2231	T3522	T3521	punct	", ",L
R2232	T3523	T3521	conj	O,L
R2233	T3524	T3523	cc	and,O
R2234	T3525	T3523	conj	P,O
R2235	T3526	T3515	punct	),K
R2236	T3527	T3484	punct	.,observed
R2237	T3529	T3530	prep	In,observed
R2238	T3531	T3529	pobj	contrast,In
R2239	T3532	T3530	punct	", ",observed
R224	T485	T484	prep	by,judged
R2240	T3533	T3530	advcl	using,observed
R2241	T3534	T3535	det	the,assay
R2242	T3535	T3533	dobj	assay,using
R2243	T3536	T3535	compound	TUNEL,assay
R2244	T3537	T3530	punct	", ",observed
R2245	T3538	T3539	det	no,differences
R2246	T3539	T3530	nsubjpass	differences,observed
R2247	T3540	T3539	amod	apparent,differences
R2248	T3541	T3539	prep	in,differences
R2249	T3542	T3543	compound	cell,apoptosis
R225	T486	T487	nmod	surfactant,protein
R2250	T3543	T3544	compound	apoptosis,rates
R2251	T3544	T3541	pobj	rates,in
R2252	T3545	T3530	auxpass	were,observed
R2253	T3546	T3530	prep	between,observed
R2254	T3547	T3548	amod	wild,type
R2255	T3548	T3550	nmod	type,lungs
R2256	T3549	T3548	punct	-,type
R2257	T3550	T3546	pobj	lungs,between
R2258	T3551	T3548	cc	and,type
R2259	T3552	T3548	conj	knockout,type
R226	T487	T488	nmod	protein,mRNAs
R2260	T3553	T3530	prep	at,observed
R2261	T3554	T3553	pobj	E18.5,at
R2262	T3555	T3556	punct	(,M
R2263	T3556	T3530	parataxis	M,observed
R2264	T3557	T3556	dep	Fig.,M
R2265	T3558	T3557	nummod	4,Fig.
R2266	T3559	T3556	punct	", ",M
R2267	T3560	T3556	advmod	cf.,M
R2268	T3561	T3556	compound	panels,M
R2269	T3562	T3556	cc	and,M
R227	T488	T485	pobj	mRNAs,by
R2270	T3563	T3556	conj	N,M
R2271	T3564	T3556	punct	),M
R2272	T3565	T3530	punct	.,observed
R2273	T3567	T3568	advmod	Thus,impaired
R2274	T3569	T3568	punct	", ",impaired
R2275	T3570	T3568	prep	at,impaired
R2276	T3571	T3572	det	the,stages
R2277	T3572	T3570	pobj	stages,at
R2278	T3573	T3572	amod	late,stages
R2279	T3574	T3572	amod	gestational,stages
R228	T489	T487	punct	(,protein
R2280	T3575	T3568	punct	", ",impaired
R2281	T3576	T3577	nmod	cell,division
R2282	T3577	T3568	nsubjpass	division,impaired
R2283	T3578	T3577	nmod	proliferation,division
R2284	T3579	T3577	punct	/,division
R2285	T3580	T3568	auxpass	was,impaired
R2286	T3581	T3568	prep	in,impaired
R2287	T3582	T3583	compound	knockout,embryos
R2288	T3583	T3581	pobj	embryos,in
R2289	T3584	T3583	prep	with,embryos
R229	T490	T487	appos	SP,protein
R2290	T3585	T3586	det	no,increase
R2291	T3586	T3584	pobj	increase,with
R2292	T3587	T3586	prep	in,increase
R2293	T3588	T3589	compound	cell,death
R2294	T3589	T3590	compound	death,rates
R2295	T3590	T3587	pobj	rates,in
R2296	T3591	T3568	punct	.,impaired
R2297	T3855	T3856	compound	Sterol,content
R2298	T3857	T3856	prep	of,content
R2299	T3858	T3859	amod	developing,lungs
R23	T266	T265	acomp	important,is
R230	T491	T488	punct	),mRNAs
R2300	T3859	T3857	pobj	lungs,of
R2301	T3860	T3856	prep	in,content
R2302	T3861	T3862	npadvmod	Dhcr7,deficient
R2303	T3862	T3863	amod	deficient,mice
R2304	T3863	T3860	pobj	mice,in
R2305	T3865	T3866	advmod	Markedly,elevated
R2306	T3866	T3867	amod	elevated,levels
R2307	T3867	T3875	nsubjpass	levels,reported
R2308	T3868	T3869	nummod	7,DHC
R2309	T3869	T3867	nmod	DHC,levels
R231	T492	T488	cc	and,mRNAs
R2310	T3870	T3869	punct	-,DHC
R2311	T3871	T3869	punct	/,DHC
R2312	T3872	T3873	nummod	8,DHC
R2313	T3873	T3869	appos	DHC,DHC
R2314	T3874	T3873	punct	-,DHC
R2315	T3876	T3867	cc	and,levels
R2316	T3877	T3878	amod	reduced,levels
R2317	T3878	T3867	conj	levels,levels
R2318	T3879	T3878	compound	body,levels
R2319	T3880	T3878	compound	cholesterol,levels
R232	T493	T494	compound	SP,C
R2320	T3881	T3875	aux	have,reported
R2321	T3882	T3875	auxpass	been,reported
R2322	T3883	T3875	advmod	previously,reported
R2323	T3884	T3875	prep	in,reported
R2324	T3885	T3886	nmod	Dhcr7,mice
R2325	T3886	T3884	pobj	mice,in
R2326	T3887	T3885	punct	-,Dhcr7
R2327	T3888	T3885	punct	/,Dhcr7
R2328	T3889	T3885	punct	-,Dhcr7
R2329	T3890	T3886	compound	P0,mice
R233	T494	T496	compound	C,immunostaining
R2330	T3891	T3892	punct	[,17
R2331	T3892	T3875	parataxis	17,reported
R2332	T3893	T3892	nummod	16,17
R2333	T3894	T3892	punct	",",17
R2334	T3895	T3892	punct	],17
R2335	T3896	T3875	punct	.,reported
R2336	T3898	T3899	det	A,amount
R2337	T3899	T3901	nsubj	amount,was
R2338	T3900	T3899	amod	significant,amount
R2339	T3902	T3899	prep	of,amount
R234	T495	T494	punct	-,C
R2340	T3903	T3902	pobj	cholesterol,of
R2341	T3904	T3901	acomp	detectable,was
R2342	T3905	T3901	prep	in,was
R2343	T3906	T3905	pobj	lungs,in
R2344	T3907	T3906	prep	of,lungs
R2345	T3908	T3909	nmod	Dhcr7,mice
R2346	T3909	T3907	pobj	mice,of
R2347	T3910	T3908	punct	-,Dhcr7
R2348	T3911	T3908	punct	/,Dhcr7
R2349	T3912	T3908	punct	-,Dhcr7
R235	T496	T488	conj	immunostaining,mRNAs
R2350	T3913	T3901	punct	", ",was
R2351	T3914	T3901	advcl	comprising,was
R2352	T3915	T3916	punct	~,60
R2353	T3916	T3917	nummod	60,%
R2354	T3917	T3914	dobj	%,comprising
R2355	T3918	T3917	cc	and,%
R2356	T3919	T3920	punct	~,30
R2357	T3920	T3921	nummod	30,%
R2358	T3921	T3917	conj	%,%
R2359	T3922	T3917	prep	of,%
R236	T497	T476	punct	.,was
R2360	T3923	T3924	amod	total,sterols
R2361	T3924	T3922	pobj	sterols,of
R2362	T3925	T3924	compound	tissue,sterols
R2363	T3926	T3914	prep	at,comprising
R2364	T3927	T3926	pobj	E13.5,at
R2365	T3928	T3927	cc	and,E13.5
R2366	T3929	T3927	conj	neonate,E13.5
R2367	T3930	T3914	punct	", ",comprising
R2368	T3931	T3914	advmod	respectively,comprising
R2369	T3932	T3901	punct	.,was
R237	T499	T500	det	A,amount
R2370	T3934	T3935	prep	In,remained
R2371	T3936	T3934	pobj	contrast,In
R2372	T3937	T3936	prep	to,contrast
R2373	T3938	T3939	det	the,doubling
R2374	T3939	T3937	pobj	doubling,to
R2375	T3940	T3939	prep	of,doubling
R2376	T3941	T3940	pobj	cholesterol,of
R2377	T3942	T3939	prep	in,doubling
R2378	T3943	T3944	det	the,lungs
R2379	T3944	T3942	pobj	lungs,in
R238	T500	T502	nsubj	amount,was
R2380	T3945	T3944	prep	of,lungs
R2381	T3946	T3947	amod	wild,type
R2382	T3947	T3948	compound	type,embryos
R2383	T3948	T3945	pobj	embryos,of
R2384	T3949	T3950	punct	(,mg
R2385	T3950	T3939	parataxis	mg,doubling
R2386	T3951	T3950	nummod	1.82,mg
R2387	T3952	T3953	punct	/,tissue
R2388	T3953	T3950	prep	tissue,mg
R2389	T3954	T3953	compound	g,tissue
R239	T501	T500	amod	significant,amount
R2390	T3955	T3950	prep	at,mg
R2391	T3956	T3955	pobj	E13.5,at
R2392	T3957	T3950	prep	to,mg
R2393	T3958	T3959	nummod	3.55,mg
R2394	T3959	T3957	pobj	mg,to
R2395	T3960	T3961	punct	/,tissue
R2396	T3961	T3959	prep	tissue,mg
R2397	T3962	T3961	compound	g,tissue
R2398	T3963	T3959	prep	at,mg
R2399	T3964	T3963	pobj	P0,at
R24	T267	T266	prep	for,important
R240	T503	T500	prep	of,amount
R2400	T3965	T3950	punct	),mg
R2401	T3966	T3935	punct	", ",remained
R2402	T3967	T3968	compound	cholesterol,content
R2403	T3968	T3935	nsubj	content,remained
R2404	T3969	T3968	prep	in,content
R2405	T3970	T3971	det	the,lungs
R2406	T3971	T3969	pobj	lungs,in
R2407	T3972	T3971	prep	of,lungs
R2408	T3973	T3974	npadvmod	Dhcr7,null
R2409	T3974	T3975	amod	null,embryos
R241	T504	T503	pobj	cholesterol,of
R2410	T3975	T3972	pobj	embryos,of
R2411	T3976	T3935	oprd	at,remained
R2412	T3977	T3978	advmod	almost,constant
R2413	T3978	T3979	amod	constant,levels
R2414	T3979	T3976	pobj	levels,at
R2415	T3980	T3979	amod	low,levels
R2416	T3981	T3935	prep	between,remained
R2417	T3982	T3983	nmod	E13.5,stages
R2418	T3983	T3981	pobj	stages,between
R2419	T3984	T3982	cc	and,E13.5
R242	T505	T502	acomp	detectable,was
R2420	T3985	T3982	conj	P0,E13.5
R2421	T3986	T3983	amod	gestational,stages
R2422	T3987	T3988	punct	(,Fig.
R2423	T3988	T3935	parataxis	Fig.,remained
R2424	T3989	T3988	nummod	5,Fig.
R2425	T3990	T3988	punct	),Fig.
R2426	T3991	T3935	punct	.,remained
R2427	T3993	T3994	expl	There,was
R2428	T3994	T3995	ccomp	was,generated
R2429	T3996	T3997	det	a,increase
R243	T506	T502	prep	in,was
R2430	T3997	T3994	attr	increase,was
R2431	T3998	T3997	amod	progressive,increase
R2432	T3999	T3997	prep	in,increase
R2433	T4000	T4001	nummod	7,DHC
R2434	T4001	T3999	pobj	DHC,in
R2435	T4002	T4001	punct	-,DHC
R2436	T4003	T4001	cc	and,DHC
R2437	T4004	T4005	nummod	8,DHC
R2438	T4005	T4001	conj	DHC,DHC
R2439	T4006	T4005	punct	-,DHC
R244	T507	T508	compound	knockout,lungs
R2440	T4007	T3995	punct	;,generated
R2441	T4008	T4009	det	the,latter
R2442	T4009	T3995	nsubjpass	latter,generated
R2443	T4010	T3995	auxpass	is,generated
R2444	T4011	T3995	prep	as,generated
R2445	T4012	T4013	det	a,isomerization
R2446	T4013	T4011	pobj	isomerization,as
R2447	T4014	T4013	amod	spontaneous,isomerization
R2448	T4015	T4013	prep	from,isomerization
R2449	T4016	T4017	det	the,former
R245	T508	T506	pobj	lungs,in
R2450	T4017	T4015	pobj	former,from
R2451	T4018	T3995	punct	.,generated
R2452	T4020	T4021	amod	Total,sterols
R2453	T4021	T4022	nsubjpass	sterols,reduced
R2454	T4023	T4021	prep	at,sterols
R2455	T4024	T4023	pobj	birth,at
R2456	T4025	T4022	auxpass	were,reduced
R2457	T4026	T4022	prep	in,reduced
R2458	T4027	T4026	pobj	lungs,in
R2459	T4028	T4027	prep	of,lungs
R246	T509	T502	punct	", ",was
R2460	T4029	T4030	det	the,mice
R2461	T4030	T4028	pobj	mice,of
R2462	T4031	T4032	npadvmod	Dhcr7,null
R2463	T4032	T4030	amod	null,mice
R2464	T4033	T4022	punct	", ",reduced
R2465	T4034	T4022	cc	but,reduced
R2466	T4035	T4022	conj	were,reduced
R2467	T4036	T4035	advmod	only,were
R2468	T4037	T4035	acomp	lower,were
R2469	T4038	T4035	prep	by,were
R247	T510	T502	advcl	implicating,was
R2470	T4039	T4040	punct	~,30
R2471	T4040	T4041	nummod	30,%
R2472	T4041	T4038	pobj	%,by
R2473	T4042	T4043	punct	(,Fig.
R2474	T4043	T4035	parataxis	Fig.,were
R2475	T4044	T4043	nummod	5,Fig.
R2476	T4045	T4043	punct	", ",Fig.
R2477	T4046	T4043	advcl	see,Fig.
R2478	T4047	T4046	dobj	values,see
R2479	T4048	T4047	prep	for,values
R248	T511	T512	det	some,transfer
R2480	T4049	T4050	compound	P0,mice
R2481	T4050	T4048	pobj	mice,for
R2482	T4051	T4043	punct	),Fig.
R2483	T4052	T4022	punct	.,reduced
R2484	T4058	T4059	amod	l,e
R2485	T4059	T4060	nmod	e,s
R2486	T4060	T4057	pobj	s,o
R2487	T4062	T4063	nsubjpass	t,r
R2488	T4064	T4063	auxpass	e,r
R2489	T4065	T4063	conj	is,r
R249	T512	T510	dobj	transfer,implicating
R2490	T4067	T4066	pobj	l,o
R2491	T4071	T4070	amod	b,s
R2492	T4076	T4075	advmod	utely,required
R2493	T4077	T4075	prep	for,required
R2494	T4078	T4079	compound	steroid,hormone
R2495	T4079	T4080	compound	hormone,synthesis
R2496	T4080	T4077	pobj	synthesis,for
R2497	T4081	T4080	prep	by,synthesis
R2498	T4082	T4083	det	the,glands
R2499	T4083	T4081	pobj	glands,by
R25	T268	T269	amod	mammalian,development
R250	T513	T512	amod	maternal,transfer
R2500	T4084	T4083	amod	adrenal,glands
R2501	T4085	T4075	punct	.,required
R2502	T4087	T4088	amod	Adrenal,insufficiency
R2503	T4088	T4089	nsubj	insufficiency,be
R2504	T4090	T4089	punct	", ",be
R2505	T4091	T4092	mark	as,reported
R2506	T4092	T4089	advcl	reported,be
R2507	T4093	T4092	aux	has,reported
R2508	T4094	T4092	auxpass	been,reported
R2509	T4095	T4092	prep	in,reported
R251	T514	T512	prep	of,transfer
R2510	T4096	T4097	det	some,cases
R2511	T4097	T4095	pobj	cases,in
R2512	T4098	T4097	amod	severe,cases
R2513	T4099	T4097	prep	of,cases
R2514	T4100	T4101	compound	SLOS,patients
R2515	T4101	T4099	pobj	patients,of
R2516	T4102	T4089	punct	", ",be
R2517	T4103	T4089	aux	could,be
R2518	T4104	T4089	acomp	responsible,be
R2519	T4105	T4104	prep	for,responsible
R252	T515	T514	pobj	cholesterol,of
R2520	T4106	T4107	det	the,hypoplasia
R2521	T4107	T4105	pobj	hypoplasia,for
R2522	T4108	T4107	compound	lung,hypoplasia
R2523	T4109	T4107	cc	and,hypoplasia
R2524	T4110	T4111	amod	perinatal,lethality
R2525	T4111	T4107	conj	lethality,hypoplasia
R2526	T4112	T4113	punct	[,30
R2527	T4113	T4089	parataxis	30,be
R2528	T4114	T4113	punct	],30
R2529	T4115	T4089	punct	.,be
R253	T516	T502	punct	.,was
R2530	T4117	T4118	nsubj	Steroids,are
R2531	T4119	T4117	punct	", ",Steroids
R2532	T4120	T4117	cc	but,Steroids
R2533	T4121	T4122	advmod	particularly,corticosteroids
R2534	T4122	T4117	conj	corticosteroids,Steroids
R2535	T4123	T4118	punct	", ",are
R2536	T4124	T4118	acomp	necessary,are
R2537	T4125	T4124	prep	for,necessary
R2538	T4126	T4127	compound	lung,maturation
R2539	T4127	T4125	pobj	maturation,for
R254	T518	T519	det	No,differences
R2540	T4128	T4127	punct	", ",maturation
R2541	T4129	T4130	advmod	especially,synthesis
R2542	T4130	T4127	appos	synthesis,maturation
R2543	T4131	T4130	det	the,synthesis
R2544	T4132	T4130	cc	and,synthesis
R2545	T4133	T4130	conj	secretion,synthesis
R2546	T4134	T4130	prep	of,synthesis
R2547	T4135	T4136	compound	surfactant,components
R2548	T4136	T4134	pobj	components,of
R2549	T4137	T4138	punct	[,31
R255	T519	T521	nsubjpass	differences,detected
R2550	T4138	T4118	parataxis	31,are
R2551	T4139	T4138	punct	],31
R2552	T4140	T4118	punct	.,are
R2553	T4142	T4143	nsubj	We,determined
R2554	T4144	T4145	compound	plasma,corticosterone
R2555	T4145	T4146	compound	corticosterone,levels
R2556	T4146	T4143	dobj	levels,determined
R2557	T4147	T4143	prep	in,determined
R2558	T4148	T4149	compound	P0,mice
R2559	T4149	T4147	pobj	mice,in
R256	T520	T519	amod	significant,differences
R2560	T4150	T4143	prep	within,determined
R2561	T4151	T4152	nummod	12,h
R2562	T4152	T4150	pobj	h,within
R2563	T4153	T4152	prep	of,h
R2564	T4154	T4153	pobj	birth,of
R2565	T4155	T4143	punct	.,determined
R2566	T4157	T4158	amod	Wild,type
R2567	T4158	T4159	compound	type,mice
R2568	T4159	T4161	nsubj	mice,had
R2569	T4160	T4159	compound	P0,mice
R257	T522	T519	prep	of,differences
R2570	T4162	T4163	compound	corticosterone,levels
R2571	T4163	T4161	dobj	levels,had
R2572	T4164	T4163	prep	of,levels
R2573	T4165	T4166	quantmod	158,50
R2574	T4166	T4168	nummod	50,ng
R2575	T4167	T4166	punct	±,50
R2576	T4168	T4164	pobj	ng,of
R2577	T4169	T4170	punct	/,ml
R2578	T4170	T4168	prep	ml,ng
R2579	T4171	T4172	punct	(,11
R258	T523	T524	det	the,localization
R2580	T4172	T4163	parataxis	11,levels
R2581	T4173	T4172	nsubj	n,11
R2582	T4174	T4172	punct	=,11
R2583	T4175	T4172	punct	),11
R2584	T4176	T4161	punct	", ",had
R2585	T4177	T4161	prep	compared,had
R2586	T4178	T4177	prep	with,compared
R2587	T4179	T4180	quantmod	225,50
R2588	T4180	T4182	nummod	50,ng
R2589	T4181	T4180	punct	±,50
R259	T524	T522	pobj	localization,of
R2590	T4182	T4178	pobj	ng,with
R2591	T4183	T4184	punct	/,ml
R2592	T4184	T4182	prep	ml,ng
R2593	T4185	T4186	punct	(,0.01
R2594	T4186	T4182	parataxis	0.01,ng
R2595	T4187	T4188	nsubj	n,7
R2596	T4188	T4186	ccomp	7,0.01
R2597	T4189	T4188	punct	=,7
R2598	T4190	T4186	punct	", ",0.01
R2599	T4191	T4186	nsubj	p,0.01
R26	T269	T267	pobj	development,for
R260	T525	T526	amod	spatial,temporal
R2600	T4192	T4186	punct	<,0.01
R2601	T4193	T4186	punct	),0.01
R2602	T4194	T4182	prep	in,ng
R2603	T4195	T4196	nmod	Dhcr7,mice
R2604	T4196	T4194	pobj	mice,in
R2605	T4197	T4195	punct	-,Dhcr7
R2606	T4198	T4195	punct	/,Dhcr7
R2607	T4199	T4195	punct	-,Dhcr7
R2608	T4200	T4196	compound	P0,mice
R2609	T4201	T4161	punct	.,had
R261	T526	T524	amod	temporal,localization
R2610	T4203	T4204	advmod	Thus,decreased
R2611	T4205	T4204	punct	", ",decreased
R2612	T4206	T4207	det	the,levels
R2613	T4207	T4204	nsubjpass	levels,decreased
R2614	T4208	T4207	compound	corticosterone,levels
R2615	T4209	T4207	prep	in,levels
R2616	T4210	T4211	nmod	Dhcr7,mice
R2617	T4211	T4209	pobj	mice,in
R2618	T4212	T4210	punct	-,Dhcr7
R2619	T4213	T4210	punct	/,Dhcr7
R262	T527	T526	punct	-,temporal
R2620	T4214	T4210	punct	-,Dhcr7
R2621	T4215	T4204	auxpass	were,decreased
R2622	T4216	T4204	neg	not,decreased
R2623	T4217	T4204	punct	", ",decreased
R2624	T4218	T4204	cc	but,decreased
R2625	T4219	T4204	conj	increased,decreased
R2626	T4220	T4219	prep	by,increased
R2627	T4221	T4222	punct	~,40
R2628	T4222	T4223	nummod	40,%
R2629	T4223	T4220	pobj	%,by
R263	T528	T524	prep	of,localization
R2630	T4224	T4204	punct	.,decreased
R2631	T4226	T4227	advmod	However,was
R2632	T4228	T4227	punct	", ",was
R2633	T4229	T4230	mark	since,dying
R2634	T4230	T4227	advcl	dying,was
R2635	T4231	T4232	det	these,mice
R2636	T4232	T4230	nsubj	mice,dying
R2637	T4233	T4232	compound	P0,mice
R2638	T4234	T4230	aux	were,dying
R2639	T4235	T4227	punct	", ",was
R264	T529	T530	amod	sonic,hedgehog
R2640	T4236	T4227	advmod	perhaps,was
R2641	T4237	T4238	det	this,increase
R2642	T4238	T4227	nsubj	increase,was
R2643	T4239	T4227	acomp	sub-optimal,was
R2644	T4240	T4227	prep	given,was
R2645	T4241	T4242	det	the,stress
R2646	T4242	T4240	pobj	stress,given
R2647	T4243	T4227	punct	.,was
R2648	T4245	T4246	advmod	Nevertheless,was
R2649	T4247	T4246	punct	", ",was
R265	T530	T528	pobj	hedgehog,of
R2650	T4248	T4249	det	an,lack
R2651	T4249	T4246	nsubj	lack,was
R2652	T4250	T4249	amod	absolute,lack
R2653	T4251	T4249	prep	of,lack
R2654	T4252	T4251	pobj	corticosterone,of
R2655	T4253	T4246	neg	not,was
R2656	T4254	T4246	acomp	present,was
R2657	T4255	T4246	prep	in,was
R2658	T4256	T4257	det	these,pups
R2659	T4257	T4255	pobj	pups,in
R266	T531	T530	punct	(,hedgehog
R2660	T4258	T4257	compound	knockout,pups
R2661	T4259	T4246	punct	.,was
R2662	T4261	T4262	aux	To,rule
R2663	T4262	T4264	advcl	rule,examined
R2664	T4263	T4262	advmod	further,rule
R2665	T4265	T4262	prt	out,rule
R2666	T4266	T4267	det	any,effects
R2667	T4267	T4262	dobj	effects,rule
R2668	T4268	T4267	prep	of,effects
R2669	T4269	T4270	compound	corticosterone,deficiency
R267	T532	T530	appos	Shh,hedgehog
R2670	T4270	T4268	pobj	deficiency,of
R2671	T4271	T4267	prep	on,effects
R2672	T4272	T4273	compound	lung,immaturity
R2673	T4273	T4271	pobj	immaturity,on
R2674	T4274	T4264	punct	", ",examined
R2675	T4275	T4264	nsubj	we,examined
R2676	T4276	T4264	dobj	lungs,examined
R2677	T4277	T4264	prep	for,examined
R2678	T4278	T4279	compound	surfactant,protein
R2679	T4279	T4280	compound	protein,expression
R268	T533	T530	punct	),hedgehog
R2680	T4280	T4277	pobj	expression,for
R2681	T4281	T4264	punct	.,examined
R2682	T4283	T4284	compound	Surfactant,C
R2683	T4284	T4286	nsubj	C,was
R2684	T4285	T4284	compound	protein,C
R2685	T4287	T4286	punct	", ",was
R2686	T4288	T4289	mark	as,determined
R2687	T4289	T4286	advcl	determined,was
R2688	T4290	T4289	prep	by,determined
R2689	T4291	T4290	pobj	IHC,by
R269	T534	T530	cc	or,hedgehog
R2690	T4292	T4286	punct	", ",was
R2691	T4293	T4286	acomp	comparable,was
R2692	T4294	T4286	prep	in,was
R2693	T4295	T4296	compound	lung,sections
R2694	T4296	T4294	pobj	sections,in
R2695	T4297	T4296	prep	from,sections
R2696	T4298	T4299	amod	wild,type
R2697	T4299	T4301	nmod	type,embryos
R2698	T4300	T4299	punct	-,type
R2699	T4301	T4297	pobj	embryos,from
R27	T270	T269	amod	embryonic,development
R270	T535	T536	poss	its,targets
R2700	T4302	T4299	cc	or,type
R2701	T4303	T4299	conj	Dhcr7,type
R2702	T4304	T4303	amod	null,Dhcr7
R2703	T4305	T4286	prep	at,was
R2704	T4306	T4307	preconj	either,E18.5
R2705	T4307	T4305	pobj	E18.5,at
R2706	T4308	T4305	cc	or,at
R2707	T4309	T4305	conj	at,at
R2708	T4310	T4309	pobj	E20.5,at
R2709	T4311	T4312	amod	gestational,ages
R271	T536	T530	conj	targets,hedgehog
R2710	T4312	T4309	pobj	ages,at
R2711	T4313	T4314	punct	(,A
R2712	T4314	T4286	parataxis	A,was
R2713	T4315	T4314	dep	Fig.,A
R2714	T4316	T4315	nummod	6,Fig.
R2715	T4317	T4314	punct	", ",A
R2716	T4318	T4314	compound	panel,A
R2717	T4319	T4314	punct	),A
R2718	T4320	T4286	punct	.,was
R2719	T4322	T4323	compound	Northern,analyses
R272	T537	T536	amod	downstream,targets
R2720	T4323	T4324	nsubj	analyses,showed
R2721	T4325	T4323	prep	for,analyses
R2722	T4326	T4327	compound	SP,A
R2723	T4327	T4325	pobj	A,for
R2724	T4328	T4327	punct	-,A
R2725	T4329	T4327	punct	", ",A
R2726	T4330	T4331	compound	SP,B
R2727	T4331	T4327	conj	B,A
R2728	T4332	T4331	punct	-,B
R2729	T4333	T4331	punct	", ",B
R273	T538	T524	prep	by,localization
R2730	T4334	T4335	compound	SP,C
R2731	T4335	T4331	conj	C,B
R2732	T4336	T4335	punct	-,C
R2733	T4337	T4335	cc	or,C
R2734	T4338	T4339	compound	SP,D
R2735	T4339	T4335	conj	D,C
R2736	T4340	T4339	punct	-,D
R2737	T4341	T4342	det	no,differences
R2738	T4342	T4324	dobj	differences,showed
R2739	T4343	T4342	advmod	significantly,differences
R274	T539	T538	pobj	immunohistochemistry,by
R2740	T4344	T4342	prep	between,differences
R2741	T4345	T4346	amod	wild,type
R2742	T4346	T4348	nmod	type,mice
R2743	T4347	T4346	punct	-,type
R2744	T4348	T4344	pobj	mice,between
R2745	T4349	T4346	cc	and,type
R2746	T4350	T4351	npadvmod	Dhcr7,null
R2747	T4351	T4346	conj	null,type
R2748	T4352	T4324	prep	at,showed
R2749	T4353	T4352	pobj	term,at
R275	T540	T521	auxpass	were,detected
R2750	T4354	T4355	punct	(,B
R2751	T4355	T4324	parataxis	B,showed
R2752	T4356	T4355	dep	Fig.,B
R2753	T4357	T4356	nummod	6,Fig.
R2754	T4358	T4355	punct	", ",B
R2755	T4359	T4355	compound	panel,B
R2756	T4360	T4355	punct	),B
R2757	T4361	T4324	punct	.,showed
R2758	T4363	T4364	advmod	Thus,altered
R2759	T4365	T4364	punct	", ",altered
R276	T541	T521	prep	between,detected
R2760	T4366	T4367	compound	surfactant,protein
R2761	T4367	T4368	compound	protein,expression
R2762	T4368	T4364	nsubjpass	expression,altered
R2763	T4369	T4364	auxpass	was,altered
R2764	T4370	T4364	neg	not,altered
R2765	T4371	T4364	agent	by,altered
R2766	T4372	T4373	compound	Dhcr7,deficiency
R2767	T4373	T4371	pobj	deficiency,by
R2768	T4374	T4364	punct	.,altered
R277	T542	T543	nmod	knockout,lungs
R2777	T4548	T4549	nmod	Dhcr7,lungs
R2778	T4549	T4553	nsubj	lungs,showed
R2779	T4550	T4548	punct	-,Dhcr7
R278	T543	T541	pobj	lungs,between
R2780	T4551	T4548	punct	/,Dhcr7
R2781	T4552	T4548	punct	-,Dhcr7
R2782	T4554	T4555	amod	abnormal,differentiation
R2783	T4555	T4553	dobj	differentiation,showed
R2784	T4556	T4555	prep	of,differentiation
R2785	T4557	T4556	pobj	type,of
R2786	T4558	T4557	nummod	I,type
R2787	T4559	T4560	amod	alveolar,cells
R2788	T4560	T4557	appos	cells,type
R2789	T4561	T4560	amod	epithelial,cells
R279	T544	T542	cc	and,knockout
R2790	T4562	T4560	punct	(,cells
R2791	T4563	T4560	appos	AECs,cells
R2792	T4564	T4560	punct	),cells
R2793	T4566	T4567	prep	At,consisted
R2794	T4568	T4566	pobj	E20.5,At
R2795	T4569	T4567	punct	", ",consisted
R2796	T4570	T4571	det	the,lung
R2797	T4571	T4567	nsubj	lung,consisted
R2798	T4572	T4571	amod	distal,lung
R2799	T4573	T4571	prep	of,lung
R28	T271	T265	punct	.,is
R280	T545	T546	amod	wild,type
R2800	T4574	T4575	amod	wild,type
R2801	T4575	T4576	compound	type,embryos
R2802	T4576	T4573	pobj	embryos,of
R2803	T4577	T4567	prep	of,consisted
R2804	T4578	T4579	amod	saccular,structures
R2805	T4579	T4577	pobj	structures,of
R2806	T4580	T4579	acl	lined,structures
R2807	T4581	T4580	prep	by,lined
R2808	T4582	T4583	amod	flat,AECs
R2809	T4583	T4581	pobj	AECs,by
R281	T546	T542	conj	type,knockout
R2810	T4584	T4583	nmod	type,AECs
R2811	T4585	T4584	nummod	I,type
R2812	T4586	T4581	punct	", ",by
R2813	T4587	T4581	cc	and,by
R2814	T4588	T4581	conj	by,by
R2815	T4589	T4590	amod	cuboidal,AECs
R2816	T4590	T4588	pobj	AECs,by
R2817	T4591	T4592	npadvmod	surfactant,producing
R2818	T4592	T4590	amod	producing,AECs
R2819	T4593	T4592	punct	-,producing
R282	T547	T546	punct	-,type
R2820	T4594	T4590	nmod	type,AECs
R2821	T4595	T4594	nummod	II,type
R2822	T4596	T4597	punct	(,Fig.
R2823	T4597	T4567	parataxis	Fig.,consisted
R2824	T4598	T4597	nummod	7A,Fig.
R2825	T4599	T4597	punct	),Fig.
R2826	T4600	T4567	punct	.,consisted
R2827	T4602	T4603	prep	In,impaired
R2828	T4604	T4602	pobj	contrast,In
R2829	T4605	T4603	punct	", ",impaired
R283	T548	T521	cc	and,detected
R2830	T4606	T4607	det	the,formation
R2831	T4607	T4603	nsubjpass	formation,impaired
R2832	T4608	T4607	prep	of,formation
R2833	T4609	T4610	det	these,saccules
R2834	T4610	T4608	pobj	saccules,of
R2835	T4611	T4610	amod	typical,saccules
R2836	T4612	T4603	auxpass	was,impaired
R2837	T4613	T4603	prep	in,impaired
R2838	T4614	T4615	npadvmod	Dhcr7,deficient
R2839	T4615	T4616	amod	deficient,lungs
R284	T549	T550	compound	Shh,autoprocessing
R2840	T4616	T4613	pobj	lungs,in
R2841	T4617	T4603	punct	.,impaired
R2842	T4619	T4620	advmod	Instead,lined
R2843	T4621	T4620	punct	", ",lined
R2844	T4622	T4623	det	these,structures
R2845	T4623	T4620	nsubjpass	structures,lined
R2846	T4624	T4623	amod	saccular,structures
R2847	T4625	T4620	auxpass	were,lined
R2848	T4626	T4620	agent	by,lined
R2849	T4627	T4628	det	a,epithelium
R285	T550	T551	nsubj	autoprocessing,occurred
R2850	T4628	T4626	pobj	epithelium,by
R2851	T4629	T4628	amod	low,epithelium
R2852	T4630	T4628	amod	columnar,epithelium
R2853	T4631	T4632	dep	that,lacked
R2854	T4632	T4628	relcl	lacked,epithelium
R2855	T4633	T4634	amod	flattened,cells
R2856	T4634	T4632	dobj	cells,lacked
R2857	T4635	T4636	npadvmod	type,like
R2858	T4636	T4634	amod	like,cells
R2859	T4637	T4635	nummod	I,type
R286	T551	T521	conj	occurred,detected
R2860	T4638	T4636	punct	-,like
R2861	T4639	T4634	prep	with,cells
R2862	T4640	T4641	det	the,presence
R2863	T4641	T4639	pobj	presence,with
R2864	T4642	T4641	prep	of,presence
R2865	T4643	T4644	amod	many,tubules
R2866	T4644	T4642	pobj	tubules,of
R2867	T4645	T4644	amod	undeveloped,tubules
R2868	T4646	T4645	cc	or,undeveloped
R2869	T4647	T4645	conj	arrested,undeveloped
R287	T552	T551	advmod	normally,occurred
R2870	T4648	T4644	amod	epithelial,tubules
R2871	T4649	T4650	punct	(,Fig.
R2872	T4650	T4620	parataxis	Fig.,lined
R2873	T4651	T4650	nummod	7B,Fig.
R2874	T4652	T4650	punct	),Fig.
R2875	T4653	T4620	punct	.,lined
R2876	T4655	T4656	advcl	Consistent,was
R2877	T4657	T4655	prep	with,Consistent
R2878	T4658	T4657	pobj	disruption,with
R2879	T4659	T4658	prep	of,disruption
R288	T553	T551	prep	in,occurred
R2880	T4660	T4661	nmod	type,AECs
R2881	T4661	T4659	pobj	AECs,of
R2882	T4662	T4660	nummod	I,type
R2883	T4663	T4656	nsubj	differentiation,was
R2884	T4664	T4665	det	the,reduction
R2885	T4665	T4656	attr	reduction,was
R2886	T4666	T4665	amod	dramatic,reduction
R2887	T4667	T4665	prep	of,reduction
R2888	T4668	T4667	pobj	expression,of
R2889	T4669	T4668	prep	of,expression
R289	T554	T553	pobj	tissues,in
R2890	T4670	T4669	pobj	T1α,of
R2891	T4671	T4670	punct	", ",T1α
R2892	T4672	T4673	det	an,marker
R2893	T4673	T4670	appos	marker,T1α
R2894	T4674	T4675	amod	apical,membrane
R2895	T4675	T4673	compound	membrane,marker
R2896	T4676	T4673	compound	protein,marker
R2897	T4677	T4673	prep	of,marker
R2898	T4678	T4679	nmod	lung,AECs
R2899	T4679	T4677	pobj	AECs,of
R29	T273	T274	amod	Neonatal,mice
R290	T555	T554	prep	from,tissues
R2900	T4680	T4679	nmod	type,AECs
R2901	T4681	T4680	nummod	I,type
R2902	T4682	T4683	punct	(,C
R2903	T4683	T4656	parataxis	C,was
R2904	T4684	T4683	dep	Fig.,C
R2905	T4685	T4684	nummod	7,Fig.
R2906	T4686	T4683	punct	", ",C
R2907	T4687	T4683	advmod	cf.,C
R2908	T4688	T4683	compound	panels,C
R2909	T4689	T4683	cc	and,C
R291	T556	T557	nmod	Dhcr7,embryos
R2910	T4690	T4683	conj	D,C
R2911	T4691	T4683	punct	),C
R2912	T4692	T4656	punct	.,was
R2913	T4694	T4695	amod	Decreased,immunostaining
R2914	T4695	T4696	nsubjpass	immunostaining,noted
R2915	T4697	T4695	prep	for,immunostaining
R2916	T4698	T4697	pobj	AQP5,for
R2917	T4699	T4698	punct	", ",AQP5
R2918	T4700	T4701	det	another,marker
R2919	T4701	T4698	appos	marker,AQP5
R292	T557	T555	pobj	embryos,from
R2920	T4702	T4701	nmod	type,marker
R2921	T4703	T4702	nummod	I,type
R2922	T4704	T4701	compound	AEC,marker
R2923	T4705	T4696	punct	", ",noted
R2924	T4706	T4696	auxpass	was,noted
R2925	T4707	T4696	advmod	also,noted
R2926	T4708	T4696	prep	in,noted
R2927	T4709	T4710	npadvmod	Dhcr7,deficient
R2928	T4710	T4711	amod	deficient,lungs
R2929	T4711	T4708	pobj	lungs,in
R293	T558	T556	punct	-,Dhcr7
R2930	T4712	T4696	prep	at,noted
R2931	T4713	T4712	pobj	E20.5,at
R2932	T4714	T4696	punct	", ",noted
R2933	T4715	T4696	prep	compared,noted
R2934	T4716	T4715	prep	to,compared
R2935	T4717	T4718	compound	control,lungs
R2936	T4718	T4716	pobj	lungs,to
R2937	T4719	T4720	punct	(,E
R2938	T4720	T4696	parataxis	E,noted
R2939	T4721	T4720	dep	Fig.,E
R294	T559	T556	punct	/,Dhcr7
R2940	T4722	T4721	nummod	7,Fig.
R2941	T4723	T4720	punct	", ",E
R2942	T4724	T4720	advmod	cf.,E
R2943	T4725	T4720	compound	panels,E
R2944	T4726	T4720	cc	and,E
R2945	T4727	T4720	conj	F,E
R2946	T4728	T4720	punct	),E
R2947	T4729	T4696	punct	.,noted
R2948	T4731	T4732	nsubjpass	Megalin,expressed
R2949	T4733	T4731	punct	", ",Megalin
R295	T560	T556	punct	-,Dhcr7
R2950	T4734	T4735	det	a,member
R2951	T4735	T4731	appos	member,Megalin
R2952	T4736	T4737	amod	low,density
R2953	T4737	T4738	nmod	density,receptor
R2954	T4738	T4735	nmod	receptor,member
R2955	T4739	T4738	nmod	lipoprotein,receptor
R2956	T4740	T4738	punct	(,receptor
R2957	T4741	T4738	appos	LDLR,receptor
R2958	T4742	T4735	punct	),member
R2959	T4743	T4744	compound	gene,family
R296	T561	T551	punct	.,occurred
R2960	T4744	T4735	compound	family,member
R2961	T4745	T4732	punct	", ",expressed
R2962	T4746	T4732	auxpass	is,expressed
R2963	T4747	T4732	agent	by,expressed
R2964	T4748	T4749	amod	embryonic,cells
R2965	T4749	T4747	pobj	cells,by
R2966	T4750	T4749	amod	epithelial,cells
R2967	T4751	T4732	cc	and,expressed
R2968	T4752	T4753	aux	can,function
R2969	T4753	T4732	conj	function,expressed
R297	T565	T566	poss	Our,data
R2970	T4754	T4753	prep	as,function
R2971	T4755	T4756	det	an,receptor
R2972	T4756	T4754	pobj	receptor,as
R2973	T4757	T4756	amod	endocytic,receptor
R2974	T4758	T4756	compound	Shh,receptor
R2975	T4759	T4760	punct	[,32
R2976	T4760	T4753	parataxis	32,function
R2977	T4761	T4760	punct	],32
R2978	T4762	T4732	punct	.,expressed
R2979	T4764	T4765	nsubj	Megalin,was
R298	T566	T567	nsubj	data,indicated
R2980	T4766	T4767	advmod	highly,expressed
R2981	T4767	T4765	acomp	expressed,was
R2982	T4768	T4765	prep	on,was
R2983	T4769	T4770	det	the,epithelium
R2984	T4770	T4768	pobj	epithelium,on
R2985	T4771	T4770	amod	distal,epithelium
R2986	T4772	T4765	prep	at,was
R2987	T4773	T4772	pobj	E20.5,at
R2988	T4774	T4765	prep	from,was
R2989	T4775	T4776	amod	wild,type
R299	T568	T569	mark	that,associated
R2990	T4776	T4778	compound	type,lungs
R2991	T4777	T4776	punct	-,type
R2992	T4778	T4774	pobj	lungs,from
R2993	T4779	T4778	punct	", ",lungs
R2994	T4780	T4781	advmod	as,as
R2995	T4781	T4778	cc	as,lungs
R2996	T4782	T4781	advmod	well,as
R2997	T4783	T4784	nmod	type,AECs
R2998	T4784	T4778	conj	AECs,lungs
R2999	T4785	T4783	nummod	I,type
R30	T274	T275	nsubj	mice,die
R300	T569	T567	ccomp	associated,indicated
R3000	T4786	T4784	acl	lining,AECs
R3001	T4787	T4788	det	the,saccules
R3002	T4788	T4786	dobj	saccules,lining
R3003	T4789	T4788	amod	developed,saccules
R3004	T4790	T4791	punct	(,Fig.
R3005	T4791	T4765	parataxis	Fig.,was
R3006	T4792	T4791	nummod	7G,Fig.
R3007	T4793	T4791	punct	),Fig.
R3008	T4794	T4765	punct	.,was
R3009	T4796	T4797	prep	In,confined
R301	T570	T571	compound	cholesterol,deficiency
R3010	T4798	T4796	pobj	contrast,In
R3011	T4799	T4797	punct	", ",confined
R3012	T4800	T4801	mark	though,detected
R3013	T4801	T4797	advcl	detected,confined
R3014	T4802	T4803	compound	megalin,staining
R3015	T4803	T4801	nsubjpass	staining,detected
R3016	T4804	T4801	auxpass	was,detected
R3017	T4805	T4801	prep	in,detected
R3018	T4806	T4807	amod	developing,saccules
R3019	T4807	T4805	pobj	saccules,in
R302	T571	T569	nsubjpass	deficiency,associated
R3020	T4808	T4801	prep	in,detected
R3021	T4809	T4810	nmod	knockout,lungs
R3022	T4810	T4808	pobj	lungs,in
R3023	T4811	T4810	amod	embryonic,lungs
R3024	T4812	T4797	punct	", ",confined
R3025	T4813	T4814	poss	its,expression
R3026	T4814	T4797	nsubjpass	expression,confined
R3027	T4815	T4797	auxpass	was,confined
R3028	T4816	T4797	prep	to,confined
R3029	T4817	T4818	amod	undifferentiated,cells
R303	T572	T571	acl	caused,deficiency
R3030	T4818	T4816	pobj	cells,to
R3031	T4819	T4818	amod	cuboidal,cells
R3032	T4820	T4821	punct	(,Fig.
R3033	T4821	T4797	parataxis	Fig.,confined
R3034	T4822	T4821	nummod	7H,Fig.
R3035	T4823	T4821	punct	),Fig.
R3036	T4824	T4797	punct	", ",confined
R3037	T4825	T4826	advmod	further,indicating
R3038	T4826	T4797	advcl	indicating,confined
R3039	T4827	T4828	det	a,delay
R304	T573	T572	agent	by,caused
R3040	T4828	T4826	dobj	delay,indicating
R3041	T4829	T4828	prep	in,delay
R3042	T4830	T4831	nmod	type,AECs
R3043	T4831	T4833	compound	AECs,differentiation
R3044	T4832	T4830	nummod	I,type
R3045	T4833	T4829	pobj	differentiation,in
R3046	T4834	T4826	prep	in,indicating
R3047	T4835	T4836	det	the,lungs
R3048	T4836	T4834	pobj	lungs,in
R3049	T4837	T4836	compound	knockout,lungs
R305	T574	T575	compound	Dhcr7,null
R3050	T4838	T4797	punct	.,confined
R3057	T5051	T5052	amod	Delayed,development
R3058	T5053	T5052	amod	vascular,development
R3059	T5054	T5052	prep	in,development
R306	T575	T573	pobj	null,by
R3060	T5055	T5056	nmod	Dhcr7,lungs
R3061	T5056	T5054	pobj	lungs,in
R3062	T5057	T5055	punct	-,Dhcr7
R3063	T5058	T5055	punct	/,Dhcr7
R3064	T5059	T5055	punct	-,Dhcr7
R3065	T5061	T5062	expl	There,is
R3066	T5063	T5064	det	a,relationship
R3067	T5064	T5062	attr	relationship,is
R3068	T5065	T5064	amod	close,relationship
R3069	T5066	T5064	prep	between,relationship
R307	T576	T569	auxpass	was,associated
R3070	T5067	T5068	compound	blood,vessel
R3071	T5068	T5069	compound	vessel,development
R3072	T5069	T5066	pobj	development,between
R3073	T5070	T5069	cc	and,development
R3074	T5071	T5072	nmod	lung,development
R3075	T5072	T5069	conj	development,development
R3076	T5073	T5072	amod	structural,development
R3077	T5074	T5062	punct	.,is
R3078	T5076	T5077	prep	At,is
R3079	T5078	T5079	amod	later,stages
R308	T577	T569	prep	with,associated
R3080	T5079	T5076	pobj	stages,At
R3081	T5080	T5079	nmod	lung,stages
R3082	T5081	T5079	amod	developmental,stages
R3083	T5082	T5077	punct	", ",is
R3084	T5083	T5084	amod	timed,development
R3085	T5084	T5077	nsubj	development,is
R3086	T5085	T5086	amod	capillary,bed
R3087	T5086	T5084	compound	bed,development
R3088	T5087	T5077	acomp	essential,is
R3089	T5088	T5087	prep	for,essential
R309	T578	T579	det	a,hypoplasia
R3090	T5089	T5090	det	the,formation
R3091	T5090	T5088	pobj	formation,for
R3092	T5091	T5090	prep	of,formation
R3093	T5092	T5093	amod	mature,exchange
R3094	T5093	T5091	pobj	exchange,of
R3095	T5094	T5093	amod	alveolar,exchange
R3096	T5095	T5093	compound	gas,exchange
R3097	T5096	T5093	punct	-,exchange
R3098	T5097	T5077	punct	", ",is
R3099	T5098	T5077	prep	with,is
R31	T276	T274	acl	lacking,mice
R310	T579	T577	pobj	hypoplasia,with
R3100	T5099	T5100	det	a,loss
R3101	T5100	T5101	nsubj	loss,separating
R3102	T5101	T5098	pcomp	separating,with
R3103	T5102	T5100	prep	of,loss
R3104	T5103	T5102	pobj	mesenchyme,of
R3105	T5104	T5105	det	the,beds
R3106	T5105	T5101	dobj	beds,separating
R3107	T5106	T5105	amod	capillary,beds
R3108	T5107	T5105	cc	and,beds
R3109	T5108	T5109	det	the,AECs
R311	T580	T579	amod	distinct,hypoplasia
R3110	T5109	T5105	conj	AECs,beds
R3111	T5110	T5111	punct	[,19
R3112	T5111	T5077	parataxis	19,is
R3113	T5112	T5111	punct	],19
R3114	T5113	T5077	punct	.,is
R3115	T5115	T5116	prep	In,was
R3116	T5117	T5118	nmod	Dhcr7,lungs
R3117	T5118	T5115	pobj	lungs,In
R3118	T5119	T5117	punct	-,Dhcr7
R3119	T5120	T5117	punct	/,Dhcr7
R312	T581	T579	nmod	lung,hypoplasia
R3120	T5121	T5117	punct	-,Dhcr7
R3121	T5122	T5118	prep	at,lungs
R3122	T5123	T5124	amod	late,stages
R3123	T5124	T5122	pobj	stages,at
R3124	T5125	T5124	amod	gestational,stages
R3125	T5126	T5116	punct	", ",was
R3126	T5127	T5128	det	the,abundance
R3127	T5128	T5116	nsubj	abundance,was
R3128	T5129	T5128	amod	relative,abundance
R3129	T5130	T5128	prep	of,abundance
R313	T582	T579	amod	saccular,hypoplasia
R3130	T5131	T5130	pobj	mesenchyme,of
R3131	T5132	T5116	acomp	consistent,was
R3132	T5133	T5132	prep	with,consistent
R3133	T5134	T5135	det	the,arrest
R3134	T5135	T5133	pobj	arrest,with
R3135	T5136	T5135	amod	general,arrest
R3136	T5137	T5135	prep	in,arrest
R3137	T5138	T5137	pobj	sacculation,in
R3138	T5139	T5116	punct	.,was
R3139	T5141	T5142	aux	To,investigate
R314	T583	T579	punct	", ",hypoplasia
R3140	T5142	T5143	advcl	investigate,performed
R3141	T5144	T5145	mark	whether,involved
R3142	T5145	T5142	ccomp	involved,investigate
R3143	T5146	T5147	det	the,sacculation
R3144	T5147	T5145	nsubj	sacculation,involved
R3145	T5148	T5147	amod	delayed,sacculation
R3146	T5149	T5147	compound	lung,sacculation
R3147	T5150	T5145	advmod	also,involved
R3148	T5151	T5152	amod	abnormal,development
R3149	T5152	T5145	dobj	development,involved
R315	T584	T579	acl	characterized,hypoplasia
R3150	T5153	T5154	compound	blood,vessel
R3151	T5154	T5152	compound	vessel,development
R3152	T5155	T5143	punct	", ",performed
R3153	T5156	T5143	nsubjpass	immunostaining,performed
R3154	T5157	T5156	prep	for,immunostaining
R3155	T5158	T5159	det	the,isoform
R3156	T5159	T5157	pobj	isoform,for
R3157	T5160	T5159	compound	α,isoform
R3158	T5161	T5159	punct	-,isoform
R3159	T5162	T5159	prep	of,isoform
R316	T585	T584	agent	by,characterized
R3160	T5163	T5162	pobj	caveolin,of
R3161	T5164	T5163	punct	-,caveolin
R3162	T5165	T5163	nummod	1,caveolin
R3163	T5166	T5159	punct	(,isoform
R3164	T5167	T5168	compound	cav,1α
R3165	T5168	T5159	appos	1α,isoform
R3166	T5169	T5168	punct	-,1α
R3167	T5170	T5156	punct	),immunostaining
R3168	T5171	T5156	cc	and,immunostaining
R3169	T5172	T5173	nmod	platelet,adhesion
R317	T586	T585	pobj	failure,by
R3170	T5173	T5156	conj	adhesion,immunostaining
R3171	T5174	T5175	amod	endothelial,cell
R3172	T5175	T5173	compound	cell,adhesion
R3173	T5176	T5173	amod	molecular,adhesion
R3174	T5177	T5173	punct	(,adhesion
R3175	T5178	T5173	appos	PECAM,adhesion
R3176	T5179	T5178	punct	-,PECAM
R3177	T5180	T5178	nummod	1,PECAM
R3178	T5181	T5173	punct	),adhesion
R3179	T5182	T5143	auxpass	were,performed
R318	T587	T588	aux	to,differentiate
R3180	T5183	T5143	prep	on,performed
R3181	T5184	T5183	pobj	sections,on
R3182	T5185	T5184	prep	from,sections
R3183	T5186	T5187	amod	wild,type
R3184	T5187	T5189	nmod	type,lungs
R3185	T5188	T5187	punct	-,type
R3186	T5189	T5185	pobj	lungs,from
R3187	T5190	T5187	cc	and,type
R3188	T5191	T5187	conj	Dhcr7,type
R3189	T5192	T5191	punct	-,Dhcr7
R319	T588	T586	acl	differentiate,failure
R3190	T5193	T5191	punct	/,Dhcr7
R3191	T5194	T5191	punct	-,Dhcr7
R3192	T5195	T5143	punct	.,performed
R3193	T5197	T5198	prep	In,expressed
R3194	T5199	T5200	det	the,lung
R3195	T5200	T5197	pobj	lung,In
R3196	T5201	T5200	amod	developing,lung
R3197	T5202	T5198	punct	", ",expressed
R3198	T5203	T5204	compound	cav,1α
R3199	T5204	T5198	nsubjpass	1α,expressed
R32	T277	T278	amod	functional,reductase
R320	T589	T588	advmod	terminally,differentiate
R3200	T5205	T5204	punct	-,1α
R3201	T5206	T5198	auxpass	is,expressed
R3202	T5207	T5198	advmod	strictly,expressed
R3203	T5208	T5198	prep	in,expressed
R3204	T5209	T5210	det	the,endothelium
R3205	T5210	T5208	pobj	endothelium,in
R3206	T5211	T5210	prep	of,endothelium
R3207	T5212	T5213	amod	developing,capillary
R3208	T5213	T5211	pobj	capillary,of
R3209	T5214	T5213	cc	and,capillary
R321	T590	T591	amod	alveolar,sacs
R3210	T5215	T5216	compound	blood,vessels
R3211	T5216	T5213	conj	vessels,capillary
R3212	T5217	T5198	punct	.,expressed
R3213	T5219	T5220	compound	Cav,1α
R3214	T5220	T5222	nsubj	1α,is
R3215	T5221	T5220	punct	-,1α
R3216	T5223	T5222	acomp	present,is
R3217	T5224	T5222	prep	in,is
R3218	T5225	T5226	det	the,AECs
R3219	T5226	T5224	pobj	AECs,in
R322	T591	T588	dobj	sacs,differentiate
R3220	T5227	T5228	advmod	terminally,differentiated
R3221	T5228	T5226	amod	differentiated,AECs
R3222	T5229	T5226	nmod	type,AECs
R3223	T5230	T5229	nummod	I,type
R3224	T5231	T5222	punct	", ",is
R3225	T5232	T5222	cc	but,is
R3226	T5233	T5222	conj	is,is
R3227	T5234	T5233	neg	not,is
R3228	T5235	T5233	acomp	detectable,is
R3229	T5236	T5235	prep	in,detectable
R323	T592	T586	punct	", ",failure
R3230	T5237	T5238	poss	its,progenitors
R3231	T5238	T5236	pobj	progenitors,in
R3232	T5239	T5238	cc	or,progenitors
R3233	T5240	T5241	nmod	type,AECs
R3234	T5241	T5238	conj	AECs,progenitors
R3235	T5242	T5240	nummod	II,type
R3236	T5243	T5244	punct	[,33
R3237	T5244	T5233	parataxis	33,is
R3238	T5245	T5244	punct	],33
R3239	T5246	T5222	punct	.,is
R324	T593	T594	det	a,differentiation
R3240	T5248	T5249	prep	At,were
R3241	T5250	T5248	pobj	E14.5,At
R3242	T5251	T5249	punct	", ",were
R3243	T5252	T5253	preconj	both,epithelium
R3244	T5253	T5249	nsubj	epithelium,were
R3245	T5254	T5253	det	the,epithelium
R3246	T5255	T5253	cc	and,epithelium
R3247	T5256	T5253	conj	mesenchyme,epithelium
R3248	T5257	T5249	acomp	negative,were
R3249	T5258	T5257	prep	for,negative
R325	T594	T586	conj	differentiation,failure
R3250	T5259	T5260	compound	cav,1α
R3251	T5260	T5262	compound	1α,staining
R3252	T5261	T5260	punct	-,1α
R3253	T5262	T5258	pobj	staining,for
R3254	T5263	T5249	punct	", ",were
R3255	T5264	T5249	cc	and,were
R3256	T5265	T5266	compound	cav,1α
R3257	T5266	T5268	nsubjpass	1α,detected
R3258	T5267	T5266	punct	-,1α
R3259	T5268	T5249	conj	detected,were
R326	T595	T594	amod	delayed,differentiation
R3260	T5269	T5268	auxpass	was,detected
R3261	T5270	T5268	prep	in,detected
R3262	T5271	T5272	det	the,vessels
R3263	T5272	T5270	pobj	vessels,in
R3264	T5273	T5272	amod	developing,vessels
R3265	T5274	T5272	compound	blood,vessels
R3266	T5275	T5272	cc	and,vessels
R3267	T5276	T5272	conj	capillaries,vessels
R3268	T5277	T5268	punct	", ",detected
R3269	T5278	T5279	mark	with,observed
R327	T596	T594	prep	of,differentiation
R3270	T5279	T5268	advcl	observed,detected
R3271	T5280	T5281	det	no,differences
R3272	T5281	T5279	nsubj	differences,observed
R3273	T5282	T5279	prep	between,observed
R3274	T5283	T5284	amod	wild,type
R3275	T5284	T5286	nmod	type,lungs
R3276	T5285	T5284	punct	-,type
R3277	T5286	T5282	pobj	lungs,between
R3278	T5287	T5284	cc	and,type
R3279	T5288	T5284	conj	Dhcr7,type
R328	T597	T598	nmod	type,AECs
R3280	T5289	T5288	punct	-,Dhcr7
R3281	T5290	T5288	punct	/,Dhcr7
R3282	T5291	T5288	punct	-,Dhcr7
R3283	T5292	T5293	punct	(,A
R3284	T5293	T5268	parataxis	A,detected
R3285	T5294	T5293	dep	Fig.,A
R3286	T5295	T5294	nummod	8,Fig.
R3287	T5296	T5293	punct	", ",A
R3288	T5297	T5293	compound	panels,A
R3289	T5298	T5293	cc	and,A
R329	T598	T596	pobj	AECs,of
R3290	T5299	T5293	conj	B,A
R3291	T5300	T5293	punct	),A
R3292	T5301	T5249	punct	.,were
R3293	T5303	T5304	compound	Cav,1α
R3294	T5304	T5306	compound	1α,immunostaining
R3295	T5305	T5304	punct	-,1α
R3296	T5306	T5307	nsubj	immunostaining,demonstrated
R3297	T5308	T5309	mark	that,proceeded
R3298	T5309	T5307	ccomp	proceeded,demonstrated
R3299	T5310	T5311	det	the,development
R33	T278	T276	dobj	reductase,lacking
R330	T599	T597	nummod	I,type
R3300	T5311	T5309	nsubj	development,proceeded
R3301	T5312	T5311	amod	vascular,development
R3302	T5313	T5309	advmod	rapidly,proceeded
R3303	T5314	T5309	prep	during,proceeded
R3304	T5315	T5316	amod	saccular,stages
R3305	T5316	T5314	pobj	stages,during
R3306	T5317	T5309	prep	in,proceeded
R3307	T5318	T5319	amod	normal,lungs
R3308	T5319	T5317	pobj	lungs,in
R3309	T5320	T5321	punct	(,E17.5
R331	T600	T594	cc	and,differentiation
R3310	T5321	T5307	parataxis	E17.5,demonstrated
R3311	T5322	T5321	cc	and,E17.5
R3312	T5323	T5321	conj	E20.5,E17.5
R3313	T5324	T5321	punct	),E17.5
R3314	T5325	T5307	punct	.,demonstrated
R3315	T5327	T5328	prep	In,observed
R3316	T5329	T5330	amod	near,term
R3317	T5330	T5332	nmod	term,lungs
R3318	T5331	T5330	punct	-,term
R3319	T5332	T5327	pobj	lungs,In
R332	T601	T602	det	an,network
R3320	T5333	T5332	amod	normal,lungs
R3321	T5334	T5335	punct	(,E20.5
R3322	T5335	T5332	parataxis	E20.5,lungs
R3323	T5336	T5335	punct	),E20.5
R3324	T5337	T5328	punct	", ",observed
R3325	T5338	T5339	det	an,network
R3326	T5339	T5328	nsubjpass	network,observed
R3327	T5340	T5339	amod	extensive,network
R3328	T5341	T5339	amod	vascular,network
R3329	T5342	T5339	punct	", ",network
R333	T602	T594	conj	network,differentiation
R3330	T5343	T5339	acl	associated,network
R3331	T5344	T5343	prep	with,associated
R3332	T5345	T5346	advmod	markedly,thinned
R3333	T5346	T5347	amod	thinned,walls
R3334	T5347	T5344	pobj	walls,with
R3335	T5348	T5347	compound	septation,walls
R3336	T5349	T5347	prep	of,walls
R3337	T5350	T5351	advmod	well,developed
R3338	T5351	T5353	amod	developed,alveoli
R3339	T5352	T5351	punct	-,developed
R334	T603	T602	amod	immature,network
R3340	T5353	T5349	pobj	alveoli,of
R3341	T5354	T5347	cc	and,walls
R3342	T5355	T5356	amod	close,apposition
R3343	T5356	T5347	conj	apposition,walls
R3344	T5357	T5356	prep	to,apposition
R3345	T5358	T5359	amod	flat,AECs
R3346	T5359	T5357	pobj	AECs,to
R3347	T5360	T5359	nmod	type,AECs
R3348	T5361	T5360	nummod	I,type
R3349	T5362	T5328	punct	", ",observed
R335	T604	T602	amod	vascular,network
R3350	T5363	T5328	auxpass	was,observed
R3351	T5364	T5365	punct	(,C
R3352	T5365	T5328	parataxis	C,observed
R3353	T5366	T5365	dep	Fig.,C
R3354	T5367	T5366	nummod	8,Fig.
R3355	T5368	T5365	punct	", ",C
R3356	T5369	T5365	compound	panels,C
R3357	T5370	T5365	cc	and,C
R3358	T5371	T5365	conj	E,C
R3359	T5372	T5365	punct	),C
R336	T605	T602	prep	at,network
R3360	T5373	T5328	punct	.,observed
R3361	T5375	T5376	prep	In,revealed
R3362	T5377	T5375	pobj	contrast,In
R3363	T5378	T5376	punct	", ",revealed
R3364	T5379	T5380	det	the,patterns
R3365	T5380	T5376	nsubj	patterns,revealed
R3366	T5381	T5382	compound	cav,1α
R3367	T5382	T5380	compound	1α,patterns
R3368	T5383	T5382	punct	-,1α
R3369	T5384	T5380	prep	in,patterns
R337	T606	T607	amod	late,stages
R3370	T5385	T5384	pobj	lungs,in
R3371	T5386	T5385	prep	from,lungs
R3372	T5387	T5388	nmod	Dhcr7,embryos
R3373	T5388	T5386	pobj	embryos,from
R3374	T5389	T5387	punct	-,Dhcr7
R3375	T5390	T5387	punct	/,Dhcr7
R3376	T5391	T5387	punct	-,Dhcr7
R3377	T5392	T5393	det	a,bed
R3378	T5393	T5376	dobj	bed,revealed
R3379	T5394	T5395	advmod	relatively,undeveloped
R338	T607	T605	pobj	stages,at
R3380	T5395	T5393	amod	undeveloped,bed
R3381	T5396	T5393	amod	pulmonary,bed
R3382	T5397	T5393	amod	capillary,bed
R3383	T5398	T5399	punct	(,D
R3384	T5399	T5376	parataxis	D,revealed
R3385	T5400	T5399	dep	Fig.,D
R3386	T5401	T5400	nummod	8,Fig.
R3387	T5402	T5399	punct	", ",D
R3388	T5403	T5399	compound	panels,D
R3389	T5404	T5399	cc	and,D
R339	T608	T607	amod	gestational,stages
R3390	T5405	T5399	conj	F,D
R3391	T5406	T5399	punct	),D
R3392	T5407	T5376	punct	.,revealed
R3393	T5409	T5410	prep	At,embedded
R3394	T5411	T5409	pobj	E20.5,At
R3395	T5412	T5410	punct	", ",embedded
R3396	T5413	T5414	compound	cav,1α
R3397	T5414	T5416	npadvmod	1α,stained
R3398	T5415	T5414	punct	-,1α
R3399	T5416	T5417	amod	stained,cells
R34	T279	T278	compound	dehydrocholesterol,reductase
R340	T609	T567	punct	.,indicated
R3400	T5417	T5410	nsubjpass	cells,embedded
R3401	T5418	T5417	prep	in,cells
R3402	T5419	T5420	compound	knockout,lungs
R3403	T5420	T5418	pobj	lungs,in
R3404	T5421	T5410	auxpass	were,embedded
R3405	T5422	T5410	prep	in,embedded
R3406	T5423	T5424	det	a,mesenchyme
R3407	T5424	T5422	pobj	mesenchyme,in
R3408	T5425	T5426	advmod	relatively,thick
R3409	T5426	T5424	amod	thick,mesenchyme
R341	T611	T612	det	The,mechanism
R3410	T5427	T5424	acl	surrounding,mesenchyme
R3411	T5428	T5429	amod	undeveloped,tubules
R3412	T5429	T5427	dobj	tubules,surrounding
R3413	T5430	T5429	amod	epithelial,tubules
R3414	T5431	T5410	punct	", ",embedded
R3415	T5432	T5410	cc	and,embedded
R3416	T5433	T5434	det	these,cells
R3417	T5434	T5436	nsubj	cells,remained
R3418	T5435	T5434	amod	epithelial,cells
R3419	T5436	T5410	conj	remained,embedded
R342	T612	T614	nsubj	mechanism,is
R3420	T5437	T5436	oprd	cuboidal,remained
R3421	T5438	T5436	punct	", ",remained
R3422	T5439	T5440	advmod	instead,of
R3423	T5440	T5436	cc	of,remained
R3424	T5441	T5436	conj	flattening,remained
R3425	T5442	T5441	prt	out,flattening
R3426	T5443	T5444	punct	(,F
R3427	T5444	T5441	parataxis	F,flattening
R3428	T5445	T5444	dep	Fig.,F
R3429	T5446	T5445	nummod	8,Fig.
R343	T613	T612	amod	molecular,mechanism
R3430	T5447	T5444	punct	", ",F
R3431	T5448	T5444	compound	panel,F
R3432	T5449	T5444	punct	),F
R3433	T5450	T5410	punct	.,embedded
R3434	T5452	T5453	nmod	PECAM,staining
R3435	T5453	T5456	nsubj	staining,confirmed
R3436	T5454	T5452	punct	-,PECAM
R3437	T5455	T5452	nummod	1,PECAM
R3438	T5457	T5453	prep	at,staining
R3439	T5458	T5457	pobj	E20.5,at
R344	T615	T612	prep	of,mechanism
R3440	T5459	T5460	det	the,development
R3441	T5460	T5456	dobj	development,confirmed
R3442	T5461	T5460	amod	delayed,development
R3443	T5462	T5460	nmod	lung,development
R3444	T5463	T5460	amod	vascular,development
R3445	T5464	T5456	prep	at,confirmed
R3446	T5465	T5466	det	these,stages
R3447	T5466	T5464	pobj	stages,at
R3448	T5467	T5466	amod	late,stages
R3449	T5468	T5466	amod	gestational,stages
R345	T616	T617	amod	impaired,development
R3450	T5469	T5456	prep	in,confirmed
R3451	T5470	T5471	npadvmod	Dhcr7,deficient
R3452	T5471	T5472	amod	deficient,embryos
R3453	T5472	T5469	pobj	embryos,in
R3454	T5473	T5474	punct	(,G
R3455	T5474	T5456	parataxis	G,confirmed
R3456	T5475	T5474	dep	Fig.,G
R3457	T5476	T5475	nummod	8,Fig.
R3458	T5477	T5474	punct	", ",G
R3459	T5478	T5474	advmod	cf.,G
R346	T617	T615	pobj	development,of
R3460	T5479	T5474	compound	panels,G
R3461	T5480	T5474	cc	and,G
R3462	T5481	T5474	conj	H,G
R3463	T5482	T5474	punct	),G
R3464	T5483	T5456	punct	.,confirmed
R3467	T5726	T5727	auxpass	Is,altered
R3468	T5728	T5729	det	the,pattern
R3469	T5729	T5727	nsubjpass	pattern,altered
R347	T618	T617	compound	lung,development
R3470	T5730	T5729	prep	of,pattern
R3471	T5731	T5732	amod	sonic,expression
R3472	T5732	T5730	pobj	expression,of
R3473	T5733	T5732	compound	hedgehog,expression
R3474	T5734	T5732	cc	or,expression
R3475	T5735	T5732	conj	signaling,expression
R3476	T5736	T5727	prep	in,altered
R3477	T5737	T5738	nmod	Dhcr7,embryos
R3478	T5738	T5736	pobj	embryos,in
R3479	T5739	T5737	punct	-,Dhcr7
R348	T619	T612	acl	associated,mechanism
R3480	T5740	T5737	punct	/,Dhcr7
R3481	T5741	T5737	punct	-,Dhcr7
R3482	T5742	T5727	punct	?,altered
R3483	T5744	T5745	nsubj	Shh,is
R3484	T5746	T5745	acomp	critical,is
R3485	T5747	T5746	prep	for,critical
R3486	T5748	T5749	amod	normal,development
R3487	T5749	T5747	pobj	development,for
R3488	T5750	T5749	amod	embryonic,development
R3489	T5751	T5745	cc	and,is
R349	T620	T619	prep	with,associated
R3490	T5752	T5753	poss	its,autoprocessing
R3491	T5753	T5754	nsubj	autoprocessing,is
R3492	T5754	T5745	conj	is,is
R3493	T5755	T5753	punct	", ",autoprocessing
R3494	T5756	T5753	acl	resulting,autoprocessing
R3495	T5757	T5756	prep	in,resulting
R3496	T5758	T5759	det	a,molecule
R3497	T5759	T5757	pobj	molecule,in
R3498	T5760	T5759	compound	cholesterol,molecule
R3499	T5761	T5762	advmod	covalently,attached
R35	T280	T278	compound	Δ7,reductase
R350	T621	T622	compound	sterol,deficiency
R3500	T5762	T5759	acl	attached,molecule
R3501	T5763	T5762	prep	at,attached
R3502	T5764	T5765	poss	its,terminus
R3503	T5765	T5763	pobj	terminus,at
R3504	T5766	T5765	compound	C,terminus
R3505	T5767	T5765	punct	-,terminus
R3506	T5768	T5754	punct	", ",is
R3507	T5769	T5770	det	a,step
R3508	T5770	T5754	attr	step,is
R3509	T5771	T5770	amod	necessary,step
R351	T622	T620	pobj	deficiency,with
R3510	T5772	T5770	prep	for,step
R3511	T5773	T5774	poss	its,function
R3512	T5774	T5772	pobj	function,for
R3513	T5775	T5774	amod	regulated,function
R3514	T5776	T5774	compound	signaling,function
R3515	T5777	T5778	punct	[,34
R3516	T5778	T5754	parataxis	34,is
R3517	T5779	T5778	nummod	23,34
R3518	T5780	T5778	punct	",",34
R3519	T5781	T5778	punct	],34
R352	T623	T622	prep	by,deficiency
R3520	T5782	T5754	punct	.,is
R3521	T5784	T5785	advmod	Thus,disrupt
R3522	T5786	T5785	punct	", ",disrupt
R3523	T5787	T5785	nsubj	disruption,disrupt
R3524	T5788	T5787	prep	of,disruption
R3525	T5789	T5790	compound	cholesterol,biosynthesis
R3526	T5790	T5788	pobj	biosynthesis,of
R3527	T5791	T5785	aux	could,disrupt
R3528	T5792	T5785	advmod	conceivably,disrupt
R3529	T5793	T5794	poss	its,pattern
R353	T624	T625	compound	Dhcr7,loss
R3530	T5794	T5785	dobj	pattern,disrupt
R3531	T5795	T5794	prep	of,pattern
R3532	T5796	T5795	pobj	distribution,of
R3533	T5797	T5785	cc	and,disrupt
R3534	T5798	T5799	advmod	thus,affect
R3535	T5799	T5785	conj	affect,disrupt
R3536	T5800	T5801	amod	normal,development
R3537	T5801	T5799	dobj	development,affect
R3538	T5802	T5785	punct	.,disrupt
R3539	T5804	T5805	aux	To,determine
R354	T625	T623	pobj	loss,by
R3540	T5805	T5806	advcl	determine,assessed
R3541	T5807	T5808	mark	whether,contributed
R3542	T5808	T5805	ccomp	contributed,determine
R3543	T5809	T5810	amod	aberrant,expression
R3544	T5810	T5808	nsubj	expression,contributed
R3545	T5811	T5810	prep	of,expression
R3546	T5812	T5811	pobj	Shh,of
R3547	T5813	T5808	prep	to,contributed
R3548	T5814	T5815	det	the,phenotype
R3549	T5815	T5813	pobj	phenotype,to
R355	T626	T614	advmod	still,is
R3550	T5816	T5815	nmod	Dhcr7,phenotype
R3551	T5817	T5816	punct	-,Dhcr7
R3552	T5818	T5816	punct	/,Dhcr7
R3553	T5819	T5816	punct	-,Dhcr7
R3554	T5820	T5815	compound	lung,phenotype
R3555	T5821	T5806	punct	", ",assessed
R3556	T5822	T5806	nsubjpass	distribution,assessed
R3557	T5823	T5822	prep	of,distribution
R3558	T5824	T5823	pobj	Shh,of
R3559	T5825	T5822	prep	in,distribution
R356	T627	T614	acomp	unknown,is
R3560	T5826	T5827	det	the,lungs
R3561	T5827	T5825	pobj	lungs,in
R3562	T5828	T5827	nmod	Dhcr7,lungs
R3563	T5829	T5828	punct	-,Dhcr7
R3564	T5830	T5828	punct	/,Dhcr7
R3565	T5831	T5828	punct	-,Dhcr7
R3566	T5832	T5828	cc	and,Dhcr7
R3567	T5833	T5834	amod	wild,type
R3568	T5834	T5828	conj	type,Dhcr7
R3569	T5835	T5834	punct	-,type
R357	T628	T614	punct	", ",is
R3570	T5836	T5806	auxpass	was,assessed
R3571	T5837	T5806	prep	from,assessed
R3572	T5838	T5837	pobj	E10.5,from
R3573	T5839	T5837	prep	to,from
R3574	T5840	T5839	pobj	P0,to
R3575	T5841	T5806	prep	by,assessed
R3576	T5842	T5841	pobj	IHC,by
R3577	T5843	T5806	advcl	using,assessed
R3578	T5844	T5845	compound	Shh,N
R3579	T5845	T5847	compound	N,antibody
R358	T629	T614	cc	but,is
R3580	T5846	T5845	punct	-,N
R3581	T5847	T5843	dobj	antibody,using
R3582	T5848	T5847	compound	peptide,antibody
R3583	T5849	T5806	punct	.,assessed
R3584	T5851	T5852	prep	At,detected
R3585	T5853	T5851	pobj	E10.5,At
R3586	T5854	T5852	punct	", ",detected
R3587	T5855	T5856	det	the,distribution
R3588	T5856	T5852	nsubjpass	distribution,detected
R3589	T5857	T5856	prep	of,distribution
R359	T630	T631	det	these,results
R3590	T5858	T5857	pobj	Shh,of
R3591	T5859	T5852	auxpass	was,detected
R3592	T5860	T5852	prep	in,detected
R3593	T5861	T5862	det	the,cells
R3594	T5862	T5860	pobj	cells,in
R3595	T5863	T5862	amod	epithelial,cells
R3596	T5864	T5862	prep	of,cells
R3597	T5865	T5866	compound	lung,buds
R3598	T5866	T5864	pobj	buds,of
R3599	T5867	T5868	punct	(,panel
R36	T281	T278	punct	-,reductase
R360	T631	T632	nsubj	results,support
R3600	T5868	T5866	parataxis	panel,buds
R3601	T5869	T5868	dep	Fig.,panel
R3602	T5870	T5869	nummod	9,Fig.
R3603	T5871	T5868	punct	", ",panel
R3604	T5872	T5868	amod	left,panel
R3605	T5873	T5868	punct	: ,panel
R3606	T5874	T5868	meta	C,panel
R3607	T5875	T5874	cc	and,C
R3608	T5876	T5874	conj	D,C
R3609	T5877	T5868	punct	),panel
R361	T632	T614	conj	support,is
R3610	T5878	T5860	punct	", ",in
R3611	T5879	T5880	advmod	as,as
R3612	T5880	T5860	cc	as,in
R3613	T5881	T5880	advmod	well,as
R3614	T5882	T5860	conj	in,in
R3615	T5883	T5884	det	the,locations
R3616	T5884	T5882	pobj	locations,in
R3617	T5885	T5886	advmod	more,typical
R3618	T5886	T5884	amod	typical,locations
R3619	T5887	T5888	npadvmod	Shh,expressing
R362	T633	T632	aux	do,support
R3620	T5888	T5884	amod	expressing,locations
R3621	T5889	T5888	punct	-,expressing
R3622	T5890	T5884	punct	", ",locations
R3623	T5891	T5892	amod	such,as
R3624	T5892	T5884	prep	as,locations
R3625	T5893	T5894	det	the,plate
R3626	T5894	T5892	pobj	plate,as
R3627	T5895	T5894	compound	floor,plate
R3628	T5896	T5894	punct	", ",plate
R3629	T5897	T5894	conj	notochord,plate
R363	T634	T632	neg	not,support
R3630	T5898	T5897	cc	and,notochord
R3631	T5899	T5900	amod	mid,gut
R3632	T5900	T5897	conj	gut,notochord
R3633	T5901	T5902	punct	(,panel
R3634	T5902	T5884	parataxis	panel,locations
R3635	T5903	T5902	dep	Fig.,panel
R3636	T5904	T5903	nummod	9,Fig.
R3637	T5905	T5902	punct	", ",panel
R3638	T5906	T5902	amod	left,panel
R3639	T5907	T5902	punct	: ,panel
R364	T635	T636	det	the,involvement
R3640	T5908	T5902	meta	A,panel
R3641	T5909	T5908	punct	-,A
R3642	T5910	T5908	dep	D,A
R3643	T5911	T5902	punct	),panel
R3644	T5912	T5852	punct	.,detected
R3645	T5914	T5915	advmod	Importantly,noted
R3646	T5916	T5915	punct	", ",noted
R3647	T5917	T5918	det	no,differences
R3648	T5918	T5915	nsubjpass	differences,noted
R3649	T5919	T5918	prep	between,differences
R365	T636	T632	dobj	involvement,support
R3650	T5920	T5921	nmod	Dhcr7,embryos
R3651	T5921	T5919	pobj	embryos,between
R3652	T5922	T5920	punct	-,Dhcr7
R3653	T5923	T5920	punct	/,Dhcr7
R3654	T5924	T5920	punct	-,Dhcr7
R3655	T5925	T5920	cc	and,Dhcr7
R3656	T5926	T5920	conj	control,Dhcr7
R3657	T5927	T5915	auxpass	were,noted
R3658	T5928	T5915	punct	.,noted
R3659	T5930	T5931	advmod	Likewise,showed
R366	T637	T636	prep	of,involvement
R3660	T5932	T5931	punct	", ",showed
R3661	T5933	T5931	nsubj	examination,showed
R3662	T5934	T5933	prep	of,examination
R3663	T5935	T5936	compound	lung,sections
R3664	T5936	T5934	pobj	sections,of
R3665	T5937	T5936	prep	from,sections
R3666	T5938	T5939	amod	increasing,ages
R3667	T5939	T5937	pobj	ages,from
R3668	T5940	T5939	amod	gestational,ages
R3669	T5941	T5939	prep	through,ages
R367	T638	T639	amod	dysregulated,pathway
R3670	T5942	T5941	prep	to,through
R3671	T5943	T5942	pobj	birth,to
R3672	T5944	T5945	det	no,differences
R3673	T5945	T5931	dobj	differences,showed
R3674	T5946	T5945	amod	significant,differences
R3675	T5947	T5945	prep	between,differences
R3676	T5948	T5949	amod	wild,type
R3677	T5949	T5951	nmod	type,tissues
R3678	T5950	T5949	punct	-,type
R3679	T5951	T5947	pobj	tissues,between
R368	T639	T637	pobj	pathway,of
R3680	T5952	T5949	cc	and,type
R3681	T5953	T5954	npadvmod	Dhcr7,deficient
R3682	T5954	T5949	conj	deficient,type
R3683	T5955	T5931	punct	.,showed
R3684	T5957	T5958	det	The,patterns
R3685	T5958	T5959	nsubj	patterns,were
R3686	T5960	T5958	prep	of,patterns
R3687	T5961	T5960	pobj	staining,of
R3688	T5962	T5958	acl	observed,patterns
R3689	T5963	T5959	acomp	comparable,were
R369	T640	T641	npadvmod	Shh,Patched
R3690	T5964	T5963	prep	to,comparable
R3691	T5965	T5964	pobj	those,to
R3692	T5966	T5967	advmod	previously,reported
R3693	T5967	T5965	acl	reported,those
R3694	T5968	T5967	prep	for,reported
R3695	T5969	T5968	pobj	Shh,for
R3696	T5970	T5967	prep	in,reported
R3697	T5971	T5972	compound	mouse,lungs
R3698	T5972	T5970	pobj	lungs,in
R3699	T5973	T5974	punct	[,25
R37	T282	T278	punct	(,reductase
R370	T641	T643	amod	Patched,Gli
R3700	T5974	T5959	parataxis	25,were
R3701	T5975	T5974	punct	],25
R3702	T5976	T5959	punct	.,were
R3703	T5978	T5979	aux	To,investigate
R3704	T5979	T5981	advcl	investigate,examined
R3705	T5980	T5979	advmod	further,investigate
R3706	T5982	T5983	compound	Shh,signaling
R3707	T5983	T5979	dobj	signaling,investigate
R3708	T5984	T5979	prep	in,investigate
R3709	T5985	T5986	det	these,embryos
R371	T642	T641	punct	-,Patched
R3710	T5986	T5984	pobj	embryos,in
R3711	T5987	T5981	punct	", ",examined
R3712	T5988	T5981	nsubj	we,examined
R3713	T5989	T5990	det	the,patterns
R3714	T5990	T5981	dobj	patterns,examined
R3715	T5991	T5990	prep	of,patterns
R3716	T5992	T5991	pobj	expression,of
R3717	T5993	T5992	prep	of,expression
R3718	T5994	T5993	pobj	Patched,of
R3719	T5995	T5994	punct	(,Patched
R372	T643	T639	compound	Gli,pathway
R3720	T5996	T5994	appos	Ptch,Patched
R3721	T5997	T5994	punct	),Patched
R3722	T5998	T5994	punct	", ",Patched
R3723	T5999	T6000	det	a,receptor
R3724	T6000	T5994	appos	receptor,Patched
R3725	T6001	T6000	amod	cognate,receptor
R3726	T6002	T6000	prep	for,receptor
R3727	T6003	T6002	pobj	Shh,for
R3728	T6004	T6000	cc	and,receptor
R3729	T6005	T6000	conj	one,receptor
R373	T644	T643	punct	-,Gli
R3730	T6006	T6007	poss	whose,expression
R3731	T6007	T6008	dep	expression,regulated
R3732	T6008	T6005	relcl	regulated,one
R3733	T6009	T6008	auxpass	is,regulated
R3734	T6010	T6008	advmod	also,regulated
R3735	T6011	T6008	agent	by,regulated
R3736	T6012	T6013	compound	Shh,signaling
R3737	T6013	T6011	pobj	signaling,by
R3738	T6014	T5981	punct	.,examined
R3739	T6016	T6017	det	The,patterns
R374	T645	T636	prep	in,involvement
R3740	T6017	T6018	nsubj	patterns,were
R3741	T6019	T6017	prep	of,patterns
R3742	T6020	T6019	pobj	Ptch,of
R3743	T6021	T6017	prep	in,patterns
R3744	T6022	T6023	compound	lung,sections
R3745	T6023	T6021	pobj	sections,in
R3746	T6024	T6023	prep	from,sections
R3747	T6025	T6026	amod	various,stages
R3748	T6026	T6024	pobj	stages,from
R3749	T6027	T6026	amod	gestational,stages
R375	T646	T645	pcomp	causing,in
R3750	T6028	T6018	advmod	also,were
R3751	T6029	T6018	acomp	indistinguishable,were
R3752	T6030	T6018	prep	between,were
R3753	T6031	T6032	amod	wild,type
R3754	T6032	T6034	nmod	type,embryos
R3755	T6033	T6032	punct	-,type
R3756	T6034	T6030	pobj	embryos,between
R3757	T6035	T6032	cc	and,type
R3758	T6036	T6032	conj	knockout,type
R3759	T6037	T6038	punct	(,panels
R376	T647	T648	det	this,defect
R3760	T6038	T6018	parataxis	panels,were
R3761	T6039	T6038	dep	Fig.,panels
R3762	T6040	T6039	nummod	9,Fig.
R3763	T6041	T6038	punct	", ",panels
R3764	T6042	T6038	amod	right,panels
R3765	T6043	T6038	punct	),panels
R3766	T6044	T6018	punct	.,were
R3767	T6046	T6047	det	The,expression
R3768	T6047	T6048	nsubj	expression,was
R3769	T6049	T6047	prep	of,expression
R377	T648	T646	dobj	defect,causing
R3770	T6050	T6049	pobj	Ptch,of
R3771	T6051	T6048	prep	in,was
R3772	T6052	T6051	pobj	concordance,in
R3773	T6053	T6052	prep	with,concordance
R3774	T6054	T6053	pobj	that,with
R3775	T6055	T6054	prep	of,that
R3776	T6056	T6055	pobj	Shh,of
R3777	T6057	T6058	punct	(,panels
R3778	T6058	T6048	parataxis	panels,was
R3779	T6059	T6058	dep	Fig,panels
R378	T649	T632	punct	.,support
R3780	T6060	T6059	nummod	9,Fig
R3781	T6061	T6058	punct	", ",panels
R3782	T6062	T6058	amod	left,panels
R3783	T6063	T6062	cc	and,left
R3784	T6064	T6062	conj	right,left
R3785	T6065	T6058	punct	),panels
R3786	T6066	T6048	punct	", ",was
R3787	T6067	T6048	advcl	showing,was
R3788	T6068	T6069	nmod	lung,cell
R3789	T6069	T6071	compound	cell,expression
R379	T240	T241	amod	Late,gestational
R3790	T6070	T6069	amod	epithelial,cell
R3791	T6071	T6067	dobj	expression,showing
R3792	T6072	T6048	punct	.,was
R3793	T6074	T6075	advmod	Downstream,are
R3794	T6076	T6074	prep	of,Downstream
R3795	T6077	T6078	det	the,cascade
R3796	T6078	T6076	pobj	cascade,of
R3797	T6079	T6080	compound	Shh,Ptch
R3798	T6080	T6078	compound	Ptch,cascade
R3799	T6081	T6080	punct	-,Ptch
R38	T283	T278	appos	Dhcr7,reductase
R3800	T6082	T6078	compound	signaling,cascade
R3801	T6083	T6075	nsubj	proteins,are
R3802	T6084	T6085	amod	such,as
R3803	T6085	T6083	prep	as,proteins
R3804	T6086	T6087	amod	smoothened,proteins
R3805	T6087	T6085	pobj	proteins,as
R3806	T6088	T6089	punct	(,Smo
R3807	T6089	T6086	parataxis	Smo,smoothened
R3808	T6090	T6089	punct	),Smo
R3809	T6091	T6086	punct	", ",smoothened
R3810	T6092	T6086	cc	and,smoothened
R3811	T6093	T6086	conj	Gli,smoothened
R3812	T6094	T6087	punct	", ",proteins
R3813	T6095	T6096	amod	key,regulators
R3814	T6096	T6087	appos	regulators,proteins
R3815	T6097	T6096	prep	of,regulators
R3816	T6098	T6099	compound	target,gene
R3817	T6099	T6101	compound	gene,expression
R3818	T6100	T6099	punct	-,gene
R3819	T6101	T6097	pobj	expression,of
R3820	T6102	T6101	prep	in,expression
R3821	T6103	T6102	pobj	response,in
R3822	T6104	T6103	prep	to,response
R3823	T6105	T6106	compound	Shh,signaling
R3824	T6106	T6104	pobj	signaling,to
R3825	T6107	T6075	punct	.,are
R3826	T6109	T6110	det	No,differences
R3827	T6110	T6111	nsubjpass	differences,noted
R3828	T6112	T6110	prep	in,differences
R3829	T6113	T6114	compound	expression,patterns
R3830	T6114	T6112	pobj	patterns,in
R3831	T6115	T6110	prep	for,differences
R3832	T6116	T6115	pobj	Smo,for
R3833	T6117	T6118	punct	(,panels
R3834	T6118	T6116	parataxis	panels,Smo
R3835	T6119	T6118	dep	Fig.,panels
R3836	T6120	T6119	nummod	10,Fig.
R3837	T6121	T6118	punct	", ",panels
R3838	T6122	T6118	amod	upper,panels
R3839	T6123	T6118	punct	),panels
R3840	T6124	T6116	punct	", ",Smo
R3841	T6125	T6116	conj	Gli1,Smo
R3842	T6126	T6127	punct	(,panels
R3843	T6127	T6125	parataxis	panels,Gli1
R3844	T6128	T6127	dep	Fig.,panels
R3845	T6129	T6128	nummod	10,Fig.
R3846	T6130	T6127	punct	", ",panels
R3847	T6131	T6127	amod	middle,panels
R3848	T6132	T6127	punct	),panels
R3849	T6133	T6125	cc	or,Gli1
R3850	T6134	T6125	conj	Gli3,Gli1
R3851	T6135	T6136	punct	(,panels
R3852	T6136	T6134	parataxis	panels,Gli3
R3853	T6137	T6136	dep	Fig.,panels
R3854	T6138	T6137	nummod	10,Fig.
R3855	T6139	T6136	punct	", ",panels
R3856	T6140	T6136	compound	bottom,panels
R3857	T6141	T6136	punct	),panels
R3858	T6142	T6111	auxpass	were,noted
R3859	T6143	T6111	prep	between,noted
R3860	T6144	T6145	amod	wild,type
R3861	T6145	T6147	nmod	type,lungs
R3862	T6146	T6145	punct	-,type
R3863	T6147	T6143	pobj	lungs,between
R3864	T6148	T6145	cc	and,type
R3865	T6149	T6145	conj	knockout,type
R3866	T6150	T6111	prep	at,noted
R3867	T6151	T6152	nmod	E13.5,days
R3868	T6152	T6150	pobj	days,at
R3869	T6153	T6151	punct	", ",E13.5
R3870	T6154	T6151	conj	E15.5,E13.5
R3871	T6155	T6154	cc	and,E15.5
R3872	T6156	T6154	conj	E17.5,E15.5
R3873	T6157	T6152	amod	gestational,days
R3874	T6158	T6111	punct	.,noted
R3875	T6322	T6323	compound	Shh,processing
R3876	T6324	T6323	compound	protein,processing
R3877	T6325	T6323	prep	in,processing
R3878	T6326	T6327	nmod	Dhcr7,embryos
R3879	T6327	T6325	pobj	embryos,in
R3880	T6328	T6326	punct	-,Dhcr7
R3881	T6329	T6326	punct	/,Dhcr7
R3882	T6330	T6326	punct	-,Dhcr7
R3883	T6332	T6333	mark	Since,appeared
R3884	T6333	T6346	advcl	appeared,evaluated
R3885	T6334	T6335	compound	Shh,expression
R3886	T6335	T6333	nsubj	expression,appeared
R3887	T6336	T6335	punct	", ",expression
R3888	T6337	T6338	advmod	as,as
R3889	T6338	T6335	cc	as,expression
R3890	T6339	T6338	advmod	well,as
R3891	T6340	T6335	conj	some,expression
R3892	T6341	T6340	prep	of,some
R3893	T6342	T6343	det	the,components
R3894	T6343	T6341	pobj	components,of
R3895	T6344	T6343	compound	Shh,components
R3896	T6345	T6343	compound	signaling,components
R3897	T6347	T6348	aux	to,be
R3898	T6348	T6333	xcomp	be,appeared
R3899	T6349	T6348	acomp	normal,be
R39	T284	T278	punct	),reductase
R3900	T6350	T6348	prep	in,be
R3901	T6351	T6352	nmod	Dhcr7,embryos
R3902	T6352	T6350	pobj	embryos,in
R3903	T6353	T6351	punct	-,Dhcr7
R3904	T6354	T6351	punct	/,Dhcr7
R3905	T6355	T6351	punct	-,Dhcr7
R3906	T6356	T6346	punct	", ",evaluated
R3907	T6357	T6358	amod	endogenous,autoprocessing
R3908	T6358	T6346	nsubjpass	autoprocessing,evaluated
R3909	T6359	T6358	prep	of,autoprocessing
R3910	T6360	T6359	pobj	Shh,of
R3911	T6361	T6362	aux	to,generate
R3912	T6362	T6358	advcl	generate,autoprocessing
R3913	T6363	T6364	det	a,fragment
R3914	T6364	T6362	dobj	fragment,generate
R3915	T6365	T6366	npadvmod	cholesterol,modified
R3916	T6366	T6364	amod	modified,fragment
R3917	T6367	T6366	punct	-,modified
R3918	T6368	T6369	npadvmod	N,terminal
R3919	T6369	T6364	amod	terminal,fragment
R3920	T6370	T6369	punct	-,terminal
R3921	T6371	T6364	prep	of,fragment
R3922	T6372	T6371	pobj	Shh,of
R3923	T6373	T6364	punct	(,fragment
R3924	T6374	T6375	compound	Shh,Np
R3925	T6375	T6364	appos	Np,fragment
R3926	T6376	T6375	punct	-,Np
R3927	T6377	T6364	punct	),fragment
R3928	T6378	T6346	auxpass	was,evaluated
R3929	T6379	T6346	punct	.,evaluated
R3930	T6381	T6382	nmod	COS,cells
R3931	T6382	T6385	nsubjpass	cells,used
R3932	T6383	T6381	punct	-,COS
R3933	T6384	T6381	nummod	1,COS
R3934	T6386	T6382	punct	", ",cells
R3935	T6387	T6382	acl	transfected,cells
R3936	T6388	T6387	prep	with,transfected
R3937	T6389	T6390	amod	full,length
R3938	T6390	T6391	compound	length,cDNA
R3939	T6391	T6388	pobj	cDNA,with
R3940	T6392	T6391	compound	Shh,cDNA
R3941	T6393	T6391	punct	(,cDNA
R3942	T6394	T6391	amod	capable,cDNA
R3943	T6395	T6394	prep	of,capable
R3944	T6396	T6397	amod	endogenous,autocleavage
R3945	T6397	T6395	pobj	autocleavage,of
R3946	T6398	T6391	punct	),cDNA
R3947	T6399	T6391	cc	or,cDNA
R3948	T6400	T6401	det	a,cDNA
R3949	T6401	T6391	conj	cDNA,cDNA
R3950	T6402	T6401	amod	truncated,cDNA
R3951	T6403	T6401	compound	Shh,cDNA
R3952	T6404	T6401	punct	(,cDNA
R3953	T6405	T6406	compound	Shh,N
R3954	T6406	T6401	appos	N,cDNA
R3955	T6407	T6406	punct	-,N
R3956	T6408	T6409	punct	", ",expression
R3957	T6409	T6401	parataxis	expression,cDNA
R3958	T6410	T6409	prep	of,expression
R3959	T6411	T6412	det	the,form
R396	T1160	T1161	nsubj	Cholesterol,is
R3960	T6412	T6410	pobj	form,of
R3961	T6413	T6412	amod	shorter,form
R3962	T6414	T6409	advmod	constitutively,expression
R3963	T6415	T6409	punct	),expression
R3964	T6416	T6385	auxpass	were,used
R3965	T6417	T6385	prep	as,used
R3966	T6418	T6417	pobj	controls,as
R3967	T6419	T6420	punct	(,1
R3968	T6420	T6385	parataxis	1,used
R3969	T6421	T6420	dep	Fig.,1
R397	T1162	T1163	det	a,constituent
R3970	T6422	T6421	nummod	11,Fig.
R3971	T6423	T6420	punct	", ",1
R3972	T6424	T6420	nmod	tracks,1
R3973	T6425	T6420	cc	and,1
R3974	T6426	T6420	conj	2,1
R3975	T6427	T6420	punct	),1
R3976	T6428	T6385	punct	.,used
R3977	T6430	T6431	nsubj	Transfection,led
R3978	T6432	T6430	prep	of,Transfection
R3979	T6433	T6434	det	the,cDNA
R398	T1163	T1161	attr	constituent,is
R3980	T6434	T6432	pobj	cDNA,of
R3981	T6435	T6436	amod	full,length
R3982	T6436	T6434	amod	length,cDNA
R3983	T6437	T6436	punct	-,length
R3984	T6438	T6434	prep	for,cDNA
R3985	T6439	T6438	pobj	Shh,for
R3986	T6440	T6431	prep	to,led
R3987	T6441	T6442	det	the,detection
R3988	T6442	T6440	pobj	detection,to
R3989	T6443	T6442	prep	of,detection
R399	T1164	T1163	amod	necessary,constituent
R3990	T6444	T6445	det	an,protein
R3991	T6445	T6443	pobj	protein,of
R3992	T6446	T6447	punct	~,20
R3993	T6447	T6448	nummod	20,kD
R3994	T6448	T6445	compound	kD,protein
R3995	T6449	T6450	dep	that,corresponds
R3996	T6450	T6445	relcl	corresponds,protein
R3997	T6451	T6450	prep	with,corresponds
R3998	T6452	T6453	det	the,Shh
R3999	T6453	T6451	pobj	Shh,with
R40	T285	T278	punct	", ",reductase
R400	T1165	T1163	compound	membrane,constituent
R4000	T6454	T6453	amod	cleaved,Shh
R4001	T6455	T6454	cc	and,cleaved
R4002	T6456	T6454	conj	tailed,cleaved
R4003	T6457	T6458	punct	(,track
R4004	T6458	T6431	parataxis	track,led
R4005	T6459	T6458	dep	Fig.,track
R4006	T6460	T6459	nummod	11,Fig.
R4007	T6461	T6458	punct	", ",track
R4008	T6462	T6458	nummod	1,track
R4009	T6463	T6458	punct	),track
R401	T1166	T1163	prep	of,constituent
R4010	T6464	T6431	punct	", ",led
R4011	T6465	T6466	mark	as,described
R4012	T6466	T6431	advcl	described,led
R4013	T6467	T6466	advmod	previously,described
R4014	T6468	T6469	punct	[,35
R4015	T6469	T6466	parataxis	35,described
R4016	T6470	T6469	punct	],35
R4017	T6471	T6431	punct	.,led
R4018	T6473	T6474	nsubj	Transfection,led
R4019	T6475	T6473	prep	of,Transfection
R402	T1167	T1168	det	all,cells
R4020	T6476	T6477	compound	COS,cells
R4021	T6477	T6475	pobj	cells,of
R4022	T6478	T6473	prep	with,Transfection
R4023	T6479	T6480	det	the,cDNA
R4024	T6480	T6478	pobj	cDNA,with
R4025	T6481	T6480	amod	constitutive,cDNA
R4026	T6482	T6480	amod	truncated,cDNA
R4027	T6483	T6484	compound	Shh,N
R4028	T6484	T6480	compound	N,cDNA
R4029	T6485	T6484	punct	-,N
R403	T1168	T1166	pobj	cells,of
R4030	T6486	T6474	prep	to,led
R4031	T6487	T6488	nummod	two,bands
R4032	T6488	T6486	pobj	bands,to
R4033	T6489	T6488	amod	slower,bands
R4034	T6490	T6488	amod	migrating,bands
R4035	T6491	T6492	dep	that,correspond
R4036	T6492	T6488	advcl	correspond,bands
R4037	T6493	T6492	prep	to,correspond
R4038	T6494	T6495	amod	truncated,Shh
R4039	T6495	T6493	pobj	Shh,to
R404	T1169	T1168	amod	mammalian,cells
R4040	T6496	T6492	punct	", ",correspond
R4041	T6497	T6492	cc	but,correspond
R4042	T6498	T6499	dep	which,thought
R4043	T6499	T6492	conj	thought,correspond
R4044	T6500	T6499	auxpass	are,thought
R4045	T6501	T6502	aux	to,generated
R4046	T6502	T6499	xcomp	generated,thought
R4047	T6503	T6502	auxpass	be,generated
R4048	T6504	T6502	agent	by,generated
R4049	T6505	T6504	pobj	differences,by
R405	T1170	T1169	punct	", ",mammalian
R4050	T6506	T6505	prep	in,differences
R4051	T6507	T6508	compound	lipid,modifications
R4052	T6508	T6506	pobj	modifications,in
R4053	T6509	T6499	punct	", ",thought
R4054	T6510	T6511	mark	as,demonstrated
R4055	T6511	T6499	advcl	demonstrated,thought
R4056	T6512	T6511	advmod	previously,demonstrated
R4057	T6513	T6514	punct	[,35
R4058	T6514	T6511	parataxis	35,demonstrated
R4059	T6515	T6514	punct	],35
R406	T1171	T1169	conj	reptile,mammalian
R4060	T6516	T6474	punct	.,led
R4061	T6518	T6519	prep	In,detected
R4062	T6520	T6521	compound	tissue,lysates
R4063	T6521	T6518	pobj	lysates,In
R4064	T6522	T6521	prep	from,lysates
R4065	T6523	T6524	amod	wild,type
R4066	T6524	T6526	nmod	type,embryos
R4067	T6525	T6524	punct	-,type
R4068	T6526	T6522	pobj	embryos,from
R4069	T6527	T6524	punct	", ",type
R407	T1172	T1171	cc	and,reptile
R4070	T6528	T6529	nmod	Dhcr7,-
R4071	T6529	T6524	appos	-,type
R4072	T6530	T6529	punct	+,-
R4073	T6531	T6529	punct	/,-
R4074	T6532	T6529	cc	and,-
R4075	T6533	T6529	conj	Dhcr7,-
R4076	T6534	T6533	punct	-,Dhcr7
R4077	T6535	T6533	punct	/,Dhcr7
R4078	T6536	T6533	punct	-,Dhcr7
R4079	T6537	T6526	prep	at,embryos
R408	T1173	T1171	conj	avian,reptile
R4080	T6538	T6537	pobj	E11.5,at
R4081	T6539	T6519	punct	", ",detected
R4082	T6540	T6541	det	a,band
R4083	T6541	T6519	nsubjpass	band,detected
R4084	T6542	T6541	acl	running,band
R4085	T6543	T6542	prep	at,running
R4086	T6544	T6545	det	the,position
R4087	T6545	T6543	pobj	position,at
R4088	T6546	T6545	amod	same,position
R4089	T6547	T6545	prep	as,position
R409	T1174	T1168	punct	", ",cells
R4090	T6548	T6549	compound	Shh,Np
R4091	T6549	T6547	pobj	Np,as
R4092	T6550	T6549	punct	-,Np
R4093	T6551	T6549	punct	(,Np
R4094	T6552	T6549	amod	autocleaved,Np
R4095	T6553	T6552	cc	and,autocleaved
R4096	T6554	T6552	conj	tailed,autocleaved
R4097	T6555	T6549	punct	),Np
R4098	T6556	T6519	auxpass	was,detected
R4099	T6557	T6519	prep	in,detected
R41	T286	T287	det	a,model
R410	T1175	T1176	advmod	as,as
R4100	T6558	T6557	pobj	all,in
R4101	T6559	T6558	prep	of,all
R4102	T6560	T6561	det	the,lysates
R4103	T6561	T6559	pobj	lysates,of
R4104	T6562	T6519	punct	.,detected
R4105	T6564	T6565	expl	There,were
R4106	T6566	T6567	det	no,differences
R4107	T6567	T6565	attr	differences,were
R4108	T6568	T6567	amod	distinguishable,differences
R4109	T6569	T6567	prep	between,differences
R411	T1176	T1168	cc	as,cells
R4110	T6570	T6569	pobj	Dhcr7,between
R4111	T6571	T6570	punct	-,Dhcr7
R4112	T6572	T6570	punct	/,Dhcr7
R4113	T6573	T6570	punct	-,Dhcr7
R4114	T6574	T6570	cc	and,Dhcr7
R4115	T6575	T6576	det	the,samples
R4116	T6576	T6570	conj	samples,Dhcr7
R4117	T6577	T6578	amod	wild,type
R4118	T6578	T6576	compound	type,samples
R4119	T6579	T6578	punct	-,type
R412	T1177	T1176	advmod	well,as
R4120	T6580	T6565	punct	", ",were
R4121	T6581	T6565	advcl	suggesting,were
R4122	T6582	T6583	mark	that,occurred
R4123	T6583	T6581	ccomp	occurred,suggesting
R4124	T6584	T6585	compound	Shh,autoprocessing
R4125	T6585	T6583	nsubj	autoprocessing,occurred
R4126	T6586	T6583	advmod	normally,occurred
R4127	T6587	T6583	punct	", ",occurred
R4128	T6588	T6583	prep	despite,occurred
R4129	T6589	T6590	det	an,deficiency
R413	T1178	T1179	det	a,organisms
R4130	T6590	T6588	pobj	deficiency,despite
R4131	T6591	T6590	amod	absolute,deficiency
R4132	T6592	T6590	prep	of,deficiency
R4133	T6593	T6592	pobj	cholesterol,of
R4134	T6594	T6590	prep	in,deficiency
R4135	T6595	T6596	amod	knockout,embryos
R4136	T6596	T6594	pobj	embryos,in
R4137	T6597	T6565	punct	.,were
R4138	T6599	T6600	advmod	More,importantly
R4139	T6600	T6601	advmod	importantly,was
R414	T1179	T1168	conj	organisms,cells
R4140	T6602	T6601	punct	", ",was
R4141	T6603	T6604	det	no,band
R4142	T6604	T6601	nsubj	band,was
R4143	T6605	T6604	acl	corresponding,band
R4144	T6606	T6605	prep	to,corresponding
R4145	T6607	T6608	det	a,Shh
R4146	T6608	T6606	pobj	Shh,to
R4147	T6609	T6610	amod	full,length
R4148	T6610	T6608	compound	length,Shh
R4149	T6611	T6610	punct	-,length
R415	T1180	T1179	amod	few,organisms
R4150	T6612	T6613	punct	(,kD
R4151	T6613	T6608	parataxis	kD,Shh
R4152	T6614	T6615	punct	~,45
R4153	T6615	T6613	nummod	45,kD
R4154	T6616	T6613	punct	),kD
R4155	T6617	T6601	acomp	present,was
R4156	T6618	T6601	prep	in,was
R4157	T6619	T6620	det	the,lysates
R4158	T6620	T6618	pobj	lysates,in
R4159	T6621	T6620	compound	lung,lysates
R416	T1181	T1179	amod	other,organisms
R4160	T6622	T6620	prep	from,lysates
R4161	T6623	T6624	det	the,embryos
R4162	T6624	T6622	pobj	embryos,from
R4163	T6625	T6624	compound	knockout,embryos
R4164	T6626	T6601	punct	", ",was
R4165	T6627	T6601	advcl	suggesting,was
R4166	T6628	T6629	mark	that,impaired
R4167	T6629	T6627	ccomp	impaired,suggesting
R4168	T6630	T6629	nsubjpass	processing,impaired
R4169	T6631	T6630	prep	of,processing
R417	T1182	T1168	punct	", ",cells
R4170	T6632	T6631	pobj	Shh,of
R4171	T6633	T6629	auxpass	was,impaired
R4172	T6634	T6629	neg	not,impaired
R4173	T6635	T6601	punct	.,was
R418	T1183	T1168	cc	but,cells
R4188	T7458	T7459	det	The,elucidation
R4189	T7459	T7460	nsubj	elucidation,improved
R419	T1184	T1183	neg	not,but
R4190	T7461	T7462	mark	that,caused
R4191	T7462	T7459	acl	caused,elucidation
R4192	T7463	T7462	nsubjpass	SLOS,caused
R4193	T7464	T7462	auxpass	is,caused
R4194	T7465	T7462	agent	by,caused
R4195	T7466	T7467	det	a,defect
R4196	T7467	T7465	pobj	defect,by
R4197	T7468	T7467	prep	in,defect
R4198	T7469	T7470	det	an,enzyme
R4199	T7470	T7468	pobj	enzyme,in
R42	T287	T278	appos	model,reductase
R420	T1185	T1186	det	all,organisms
R4200	T7471	T7470	amod	necessary,enzyme
R4201	T7472	T7471	prep	for,necessary
R4202	T7473	T7474	compound	cholesterol,biosynthesis
R4203	T7474	T7472	pobj	biosynthesis,for
R4204	T7475	T7460	aux	has,improved
R4205	T7476	T7477	poss	our,understanding
R4206	T7477	T7460	dobj	understanding,improved
R4207	T7478	T7477	prep	of,understanding
R4208	T7479	T7480	amod	embryonic,development
R4209	T7480	T7478	pobj	development,of
R421	T1186	T1168	conj	organisms,cells
R4210	T7481	T7460	punct	.,improved
R4211	T7483	T7484	det	This,discovery
R4212	T7484	T7485	nsubj	discovery,led
R4213	T7486	T7485	aux	has,led
R4214	T7487	T7485	prep	to,led
R4215	T7488	T7489	det	the,identification
R4216	T7489	T7487	pobj	identification,to
R4217	T7490	T7489	prep	of,identification
R4218	T7491	T7492	amod	other,disorders
R4219	T7492	T7490	pobj	disorders,of
R422	T1187	T1186	amod	eukaryotic,organisms
R4220	T7493	T7492	prep	of,disorders
R4221	T7494	T7495	amod	embryonic,development
R4222	T7495	T7493	pobj	development,of
R4223	T7496	T7492	acl	caused,disorders
R4224	T7497	T7496	agent	by,caused
R4225	T7498	T7497	pobj	mutations,by
R4226	T7499	T7498	prep	in,mutations
R4227	T7500	T7499	pobj	genes,in
R4228	T7501	T7500	acl	encoding,genes
R4229	T7502	T7503	amod	other,enzymes
R423	T1188	T1161	punct	.,is
R4230	T7503	T7501	dobj	enzymes,encoding
R4231	T7504	T7501	prep	in,encoding
R4232	T7505	T7506	det	the,steps
R4233	T7506	T7504	pobj	steps,in
R4234	T7507	T7506	amod	post-squalene,steps
R4235	T7508	T7506	prep	of,steps
R4236	T7509	T7510	compound	cholesterol,biosynthesis
R4237	T7510	T7508	pobj	biosynthesis,of
R4238	T7511	T7485	punct	.,led
R4239	T7513	T7514	mark	Although,implicate
R424	T1190	T1191	prep	In,seem
R4240	T7514	T7519	advcl	implicate,remains
R4241	T7515	T7516	det	these,studies
R4242	T7516	T7514	nsubj	studies,implicate
R4243	T7517	T7516	amod	genetic,studies
R4244	T7518	T7514	advmod	now,implicate
R4245	T7520	T7521	det	the,need
R4246	T7521	T7514	dobj	need,implicate
R4247	T7522	T7521	prep	for,need
R4248	T7523	T7524	amod	normal,synthesis
R4249	T7524	T7522	pobj	synthesis,for
R425	T1192	T1193	det	the,cases
R4250	T7525	T7524	compound	cholesterol,synthesis
R4251	T7526	T7519	punct	", ",remains
R4252	T7527	T7528	det	the,role
R4253	T7528	T7519	nsubj	role,remains
R4254	T7529	T7528	amod	mechanistic,role
R4255	T7530	T7528	punct	(,role
R4256	T7531	T7528	nmod	s,role
R4257	T7532	T7528	punct	),role
R4258	T7533	T7528	prep	of,role
R4259	T7534	T7533	pobj	cholesterol,of
R426	T1193	T1190	pobj	cases,In
R4260	T7535	T7528	prep	in,role
R4261	T7536	T7537	amod	normal,development
R4262	T7537	T7535	pobj	development,in
R4263	T7538	T7537	amod	embryonic,development
R4264	T7539	T7540	aux	to,clarified
R4265	T7540	T7519	xcomp	clarified,remains
R4266	T7541	T7540	auxpass	be,clarified
R4267	T7542	T7519	punct	.,remains
R4268	T7544	T7545	prep	In,report
R4269	T7546	T7547	det	the,study
R427	T1194	T1193	amod	latter,cases
R4270	T7547	T7544	pobj	study,In
R4271	T7548	T7547	amod	present,study
R4272	T7549	T7545	punct	", ",report
R4273	T7550	T7545	nsubj	we,report
R4274	T7551	T7552	det	the,characterization
R4275	T7552	T7545	dobj	characterization,report
R4276	T7553	T7552	prep	of,characterization
R4277	T7554	T7555	compound	lung,development
R4278	T7555	T7553	pobj	development,of
R4279	T7556	T7552	prep	in,characterization
R428	T1195	T1193	punct	", ",cases
R4280	T7557	T7558	nmod	Dhcr7,embryos
R4281	T7558	T7556	pobj	embryos,in
R4282	T7559	T7557	punct	-,Dhcr7
R4283	T7560	T7557	punct	/,Dhcr7
R4284	T7561	T7557	punct	-,Dhcr7
R4285	T7562	T7545	punct	.,report
R4286	T7564	T7565	mark	Although,are
R4287	T7565	T7568	advcl	are,observed
R4288	T7566	T7567	amod	cardiac,abnormalities
R4289	T7567	T7565	nsubj	abnormalities,are
R429	T1196	T1197	amod	such,as
R4290	T7569	T7565	acomp	common,are
R4291	T7570	T7565	prep	in,are
R4292	T7571	T7572	amod	human,SLOS
R4293	T7572	T7570	pobj	SLOS,in
R4294	T7573	T7574	punct	[,36
R4295	T7574	T7565	parataxis	36,are
R4296	T7575	T7574	punct	],36
R4297	T7576	T7568	punct	", ",observed
R4298	T7577	T7578	det	no,abnormalities
R4299	T7578	T7568	nsubjpass	abnormalities,observed
R43	T288	T287	prep	for,model
R430	T1197	T1193	prep	as,cases
R4300	T7579	T7578	amod	structural,abnormalities
R4301	T7580	T7578	prep	of,abnormalities
R4302	T7581	T7582	det	the,heart
R4303	T7582	T7580	pobj	heart,of
R4304	T7583	T7582	cc	or,heart
R4305	T7584	T7585	det	the,vessels
R4306	T7585	T7582	conj	vessels,heart
R4307	T7586	T7585	amod	great,vessels
R4308	T7587	T7568	auxpass	were,observed
R4309	T7588	T7568	prep	in,observed
R431	T1198	T1197	pobj	plants,as
R4310	T7589	T7590	det	the,pups
R4311	T7590	T7588	pobj	pups,in
R4312	T7591	T7590	nmod	Dhcr7,pups
R4313	T7592	T7591	punct	-,Dhcr7
R4314	T7593	T7591	punct	/,Dhcr7
R4315	T7594	T7591	punct	-,Dhcr7
R4316	T7595	T7568	punct	.,observed
R4317	T7597	T7598	compound	Lung,development
R4318	T7598	T7599	nsubj	development,was
R4319	T7600	T7598	prep	in,development
R432	T1199	T1198	cc	and,plants
R4320	T7601	T7602	nmod	Dhcr7,embryos
R4321	T7602	T7600	pobj	embryos,in
R4322	T7603	T7601	punct	-,Dhcr7
R4323	T7604	T7601	punct	/,Dhcr7
R4324	T7605	T7601	punct	-,Dhcr7
R4325	T7606	T7598	prep	at,development
R4326	T7607	T7608	det	the,stages
R4327	T7608	T7606	pobj	stages,at
R4328	T7609	T7608	amod	early,stages
R4329	T7610	T7598	punct	", ",development
R433	T1200	T1198	conj	fungi,plants
R4330	T7611	T7598	prep	from,development
R4331	T7612	T7613	compound	lung,bud
R4332	T7613	T7614	compound	bud,formation
R4333	T7614	T7611	pobj	formation,from
R4334	T7615	T7611	prep	to,from
R4335	T7616	T7617	amod	canalicular,stage
R4336	T7617	T7615	pobj	stage,to
R4337	T7618	T7599	punct	", ",was
R4338	T7619	T7620	advmod	morphologically,similar
R4339	T7620	T7599	acomp	similar,was
R434	T1201	T1191	punct	", ",seem
R4340	T7621	T7620	prep	to,similar
R4341	T7622	T7621	pobj	controls,to
R4342	T7623	T7599	punct	.,was
R4343	T7625	T7626	advmod	Instead,observed
R4344	T7627	T7626	punct	", ",observed
R4345	T7628	T7629	det	a,hypoplasia
R4346	T7629	T7626	nsubjpass	hypoplasia,observed
R4347	T7630	T7629	amod	distinct,hypoplasia
R4348	T7631	T7629	compound	lung,hypoplasia
R4349	T7632	T7629	prep	at,hypoplasia
R435	T1202	T1203	npadvmod	cholesterol,like
R4350	T7633	T7634	det	the,stage
R4351	T7634	T7632	pobj	stage,at
R4352	T7635	T7634	amod	saccular,stage
R4353	T7636	T7629	punct	", ",hypoplasia
R4354	T7637	T7629	acl	characterized,hypoplasia
R4355	T7638	T7637	agent	by,characterized
R4356	T7639	T7640	amod	arrested,tubules
R4357	T7640	T7638	pobj	tubules,by
R4358	T7641	T7639	cc	or,arrested
R4359	T7642	T7643	advmod	partially,developed
R436	T1203	T1205	amod	like,sterols
R4360	T7643	T7639	conj	developed,arrested
R4361	T7644	T7640	amod	distal,tubules
R4362	T7645	T7640	amod	epithelial,tubules
R4363	T7646	T7640	punct	", ",tubules
R4364	T7647	T7648	amod	reduced,space
R4365	T7648	T7640	conj	space,tubules
R4366	T7649	T7648	amod	terminal,space
R4367	T7650	T7648	compound	sac,space
R4368	T7651	T7648	punct	", ",space
R4369	T7652	T7653	amod	delayed,differentiation
R437	T1204	T1203	punct	-,like
R4370	T7653	T7648	conj	differentiation,space
R4371	T7654	T7653	nmod	type,differentiation
R4372	T7655	T7654	nummod	I,type
R4373	T7656	T7653	compound	AECs,differentiation
R4374	T7657	T7653	cc	and,differentiation
R4375	T7658	T7659	amod	delayed,development
R4376	T7659	T7653	conj	development,differentiation
R4377	T7660	T7659	amod	vascular,development
R4378	T7661	T7626	punct	", ",observed
R4379	T7662	T7626	auxpass	was,observed
R438	T1205	T1191	nsubj	sterols,seem
R4380	T7663	T7626	advmod	consistently,observed
R4381	T7664	T7626	prep	at,observed
R4382	T7665	T7666	amod	late,stages
R4383	T7666	T7664	pobj	stages,at
R4384	T7667	T7666	amod	gestational,stages
R4385	T7668	T7626	prep	in,observed
R4386	T7669	T7670	nmod	Dhcr7,embryos
R4387	T7670	T7668	pobj	embryos,in
R4388	T7671	T7669	punct	-,Dhcr7
R4389	T7672	T7669	punct	/,Dhcr7
R439	T1206	T1205	punct	(,sterols
R4390	T7673	T7669	punct	-,Dhcr7
R4391	T7674	T7626	punct	.,observed
R4392	T7676	T7677	prep	At,undergoes
R4393	T7678	T7679	amod	late,stages
R4394	T7679	T7676	pobj	stages,At
R4395	T7680	T7679	prep	of,stages
R4396	T7681	T7682	amod	normal,development
R4397	T7682	T7680	pobj	development,of
R4398	T7683	T7682	amod	fetal,development
R4399	T7684	T7677	punct	", ",undergoes
R44	T289	T290	det	the,disease
R440	T1207	T1205	appos	phytosterols,sterols
R4400	T7685	T7686	det	the,epithelium
R4401	T7686	T7677	nsubj	epithelium,undergoes
R4402	T7687	T7686	amod	mammalian,epithelium
R4403	T7688	T7686	amod	alveolar,epithelium
R4404	T7689	T7690	det	an,differentiation
R4405	T7690	T7677	dobj	differentiation,undergoes
R4406	T7691	T7690	amod	abrupt,differentiation
R4407	T7692	T7677	prep	as,undergoes
R4408	T7693	T7694	det	a,part
R4409	T7694	T7692	pobj	part,as
R441	T1208	T1207	punct	", ",phytosterols
R4410	T7695	T7694	prep	of,part
R4411	T7696	T7697	det	the,preparation
R4412	T7697	T7695	pobj	preparation,of
R4413	T7698	T7697	prep	of,preparation
R4414	T7699	T7700	det	the,lungs
R4415	T7700	T7698	pobj	lungs,of
R4416	T7701	T7697	prep	for,preparation
R4417	T7702	T7703	det	the,demands
R4418	T7703	T7701	pobj	demands,for
R4419	T7704	T7703	amod	postnatal,demands
R442	T1209	T1207	conj	ergosterol,phytosterols
R4420	T7705	T7703	prep	of,demands
R4421	T7706	T7707	compound	gas,exchange
R4422	T7707	T7705	pobj	exchange,of
R4423	T7708	T7709	punct	[,37
R4424	T7709	T7677	parataxis	37,undergoes
R4425	T7710	T7709	punct	],37
R4426	T7711	T7677	punct	.,undergoes
R4427	T7713	T7714	preconj	Both,AECs
R4428	T7714	T7717	nsubj	AECs,are
R4429	T7715	T7714	nmod	type,AECs
R443	T1210	T1209	advmod	etc.,ergosterol
R4430	T7716	T7715	nummod	II,type
R4431	T7718	T7714	punct	", ",AECs
R4432	T7719	T7714	acl	producing,AECs
R4433	T7720	T7721	amod	pulmonary,surfactants
R4434	T7721	T7719	dobj	surfactants,producing
R4435	T7722	T7714	punct	", ",AECs
R4436	T7723	T7714	cc	and,AECs
R4437	T7724	T7725	nmod	type,AECs
R4438	T7725	T7714	conj	AECs,AECs
R4439	T7726	T7724	nummod	I,type
R444	T1211	T1205	punct	),sterols
R4440	T7727	T7725	punct	", ",AECs
R4441	T7728	T7725	acl	lining,AECs
R4442	T7729	T7730	det	the,alveoli
R4443	T7730	T7728	dobj	alveoli,lining
R4444	T7731	T7730	amod	expanded,alveoli
R4445	T7732	T7717	punct	", ",are
R4446	T7733	T7717	acomp	important,are
R4447	T7734	T7733	prep	for,important
R4448	T7735	T7736	amod	alveolar,maturation
R4449	T7736	T7734	pobj	maturation,for
R445	T1212	T1213	aux	to,substitute
R4450	T7737	T7717	punct	.,are
R4451	T7739	T7740	prep	In,is
R4452	T7741	T7739	pobj	addition,In
R4453	T7742	T7740	punct	", ",is
R4454	T7743	T7744	det	the,development
R4455	T7744	T7740	nsubj	development,is
R4456	T7745	T7744	amod	vascular,development
R4457	T7746	T7744	prep	of,development
R4458	T7747	T7746	pobj	alveoli,of
R4459	T7748	T7740	advmod	also,is
R446	T1213	T1191	xcomp	substitute,seem
R4460	T7749	T7740	acomp	essential,is
R4461	T7750	T7749	prep	for,essential
R4462	T7751	T7752	amod	normal,morphogenesis
R4463	T7752	T7750	pobj	morphogenesis,for
R4464	T7753	T7752	compound	lung,morphogenesis
R4465	T7754	T7740	prep	at,is
R4466	T7755	T7756	amod	late,stages
R4467	T7756	T7754	pobj	stages,at
R4468	T7757	T7756	amod	gestational,stages
R4469	T7758	T7759	punct	[,19
R447	T1214	T1213	prep	for,substitute
R4470	T7759	T7740	parataxis	19,is
R4471	T7760	T7759	punct	],19
R4472	T7761	T7740	punct	.,is
R4473	T7763	T7764	nsubj	We,show
R4474	T7765	T7764	advmod	here,show
R4475	T7766	T7767	mark	that,blocked
R4476	T7767	T7764	ccomp	blocked,show
R4477	T7768	T7769	det	the,differentiation
R4478	T7769	T7767	nsubjpass	differentiation,blocked
R4479	T7770	T7769	prep	of,differentiation
R448	T1215	T1214	pobj	cholesterol,for
R4480	T7771	T7772	nmod	type,AECs
R4481	T7772	T7770	pobj	AECs,of
R4482	T7773	T7771	nummod	I,type
R4483	T7774	T7772	cc	but,AECs
R4484	T7775	T7774	neg	not,but
R4485	T7776	T7777	nmod	type,AECs
R4486	T7777	T7772	conj	AECs,AECs
R4487	T7778	T7776	nummod	II,type
R4488	T7779	T7769	prep	in,differentiation
R4489	T7780	T7781	nmod	Dhcr7,lungs
R449	T1216	T1191	punct	.,seem
R4490	T7781	T7779	pobj	lungs,in
R4491	T7782	T7780	punct	-,Dhcr7
R4492	T7783	T7780	punct	/,Dhcr7
R4493	T7784	T7780	punct	-,Dhcr7
R4494	T7785	T7767	auxpass	was,blocked
R4495	T7786	T7767	cc	or,blocked
R4496	T7787	T7767	conj	delayed,blocked
R4497	T7788	T7787	punct	", ",delayed
R4498	T7789	T7790	mark	as,indicated
R4499	T7790	T7787	conj	indicated,delayed
R45	T290	T288	pobj	disease,for
R450	T1218	T1219	det	The,need
R4500	T7791	T7790	prep	by,indicated
R4501	T7792	T7793	amod	fewer,cells
R4502	T7793	T7791	pobj	cells,by
R4503	T7794	T7793	amod	flattened,cells
R4504	T7795	T7793	nmod	type,cells
R4505	T7796	T7795	nummod	I,type
R4506	T7797	T7791	cc	and,by
R4507	T7798	T7791	conj	by,by
R4508	T7799	T7800	amod	reduced,expression
R4509	T7800	T7798	pobj	expression,by
R451	T1219	T1221	nsubj	need,remains
R4510	T7801	T7800	prep	of,expression
R4511	T7802	T7801	pobj	markers,of
R4512	T7803	T7802	prep	of,markers
R4513	T7804	T7805	nmod	type,AECs
R4514	T7805	T7803	pobj	AECs,of
R4515	T7806	T7804	nummod	I,type
R4516	T7807	T7805	punct	", ",AECs
R4517	T7808	T7805	conj	T1α,AECs
R4518	T7809	T7808	cc	and,T1α
R4519	T7810	T7808	conj	AQP5,T1α
R452	T1220	T1219	amod	precise,need
R4520	T7811	T7764	punct	.,show
R4521	T7813	T7814	amod	Delayed,development
R4522	T7814	T7816	nsubjpass	development,involved
R4523	T7815	T7814	amod	vascular,development
R4524	T7817	T7816	auxpass	was,involved
R4525	T7818	T7816	advmod	also,involved
R4526	T7819	T7816	prep	in,involved
R4527	T7820	T7821	det	the,sacculation
R4528	T7821	T7819	pobj	sacculation,in
R4529	T7822	T7821	amod	delayed,sacculation
R453	T1222	T1219	prep	for,need
R4530	T7823	T7821	prep	of,sacculation
R4531	T7824	T7825	nmod	Dhcr7,lungs
R4532	T7825	T7823	pobj	lungs,of
R4533	T7826	T7824	punct	-,Dhcr7
R4534	T7827	T7824	punct	/,Dhcr7
R4535	T7828	T7824	punct	-,Dhcr7
R4536	T7829	T7816	punct	.,involved
R4537	T7831	T7832	advmod	Additionally,remained
R4538	T7833	T7832	punct	", ",remained
R4539	T7834	T7835	det	the,mesenchyme
R454	T1223	T1222	pobj	sterols,for
R4540	T7835	T7832	nsubj	mesenchyme,remained
R4541	T7836	T7835	acl	separating,mesenchyme
R4542	T7837	T7838	det	the,AECs
R4543	T7838	T7836	dobj	AECs,separating
R4544	T7839	T7836	prep	from,separating
R4545	T7840	T7841	det	the,beds
R4546	T7841	T7839	pobj	beds,from
R4547	T7842	T7841	amod	vascular,beds
R4548	T7843	T7832	oprd	thickened,remained
R4549	T7844	T7832	punct	.,remained
R455	T1224	T1219	prep	in,need
R4550	T7846	T7847	prep	In,noted
R4551	T7848	T7846	pobj	support,In
R4552	T7849	T7848	prep	of,support
R4553	T7850	T7851	amod	normal,differentiation
R4554	T7851	T7849	pobj	differentiation,of
R4555	T7852	T7851	prep	of,differentiation
R4556	T7853	T7854	nmod	type,AECs
R4557	T7854	T7852	pobj	AECs,of
R4558	T7855	T7853	nummod	II,type
R4559	T7856	T7847	punct	", ",noted
R456	T1225	T1226	amod	biological,membranes
R4560	T7857	T7858	det	no,alteration
R4561	T7858	T7847	nsubjpass	alteration,noted
R4562	T7859	T7858	prep	in,alteration
R4563	T7860	T7861	det	the,patterns
R4564	T7861	T7859	pobj	patterns,in
R4565	T7862	T7863	compound	gene,expression
R4566	T7863	T7861	compound	expression,patterns
R4567	T7864	T7861	prep	for,patterns
R4568	T7865	T7866	compound	surfactant,A
R4569	T7866	T7864	pobj	A,for
R457	T1226	T1224	pobj	membranes,in
R4570	T7867	T7866	compound	proteins,A
R4571	T7868	T7866	punct	", ",A
R4572	T7869	T7866	conj	B,A
R4573	T7870	T7869	punct	", ",B
R4574	T7871	T7869	conj	C,B
R4575	T7872	T7871	cc	or,C
R4576	T7873	T7871	conj	D,C
R4577	T7874	T7847	auxpass	was,noted
R4578	T7875	T7847	punct	", ",noted
R4579	T7876	T7847	cc	nor,noted
R458	T1227	T1221	advmod	still,remains
R4580	T7877	T7878	auxpass	was,altered
R4581	T7878	T7847	conj	altered,noted
R4582	T7879	T7880	det	the,pattern
R4583	T7880	T7878	nsubjpass	pattern,altered
R4584	T7881	T7880	prep	of,pattern
R4585	T7882	T7881	pobj	distribution,of
R4586	T7883	T7882	prep	of,distribution
R4587	T7884	T7885	compound	SP,C
R4588	T7885	T7883	pobj	C,of
R4589	T7886	T7885	punct	-,C
R459	T1228	T1229	advmod	poorly,defined
R4590	T7887	T7878	prep	in,altered
R4591	T7888	T7887	pobj	lungs,in
R4592	T7889	T7888	prep	from,lungs
R4593	T7890	T7891	compound	knockout,embryos
R4594	T7891	T7889	pobj	embryos,from
R4595	T7892	T7847	punct	.,noted
R4596	T7894	T7895	advmod	Thus,be
R4597	T7896	T7895	punct	", ",be
R4598	T7897	T7898	amod	impaired,exchange
R4599	T7898	T7895	nsubj	exchange,be
R46	T291	T290	amod	human,disease
R460	T1229	T1221	oprd	defined,remains
R4600	T7899	T7898	compound	gas,exchange
R4601	T7900	T7895	punct	", ",be
R4602	T7901	T7902	mark	as,opposed
R4603	T7902	T7895	advcl	opposed,be
R4604	T7903	T7902	prep	to,opposed
R4605	T7904	T7905	amod	poor,inflation
R4606	T7905	T7903	pobj	inflation,to
R4607	T7906	T7905	cc	and,inflation
R4608	T7907	T7908	amod	alveolar,tension
R4609	T7908	T7905	conj	tension,inflation
R461	T1230	T1221	punct	", ",remains
R4610	T7909	T7895	punct	", ",be
R4611	T7910	T7895	aux	may,be
R4612	T7911	T7912	det	the,cause
R4613	T7912	T7895	attr	cause,be
R4614	T7913	T7912	prep	of,cause
R4615	T7914	T7915	amod	respiratory,failure
R4616	T7915	T7913	pobj	failure,of
R4617	T7916	T7895	prep	in,be
R4618	T7917	T7918	det	these,pups
R4619	T7918	T7916	pobj	pups,in
R462	T1231	T1232	mark	though,be
R4620	T7919	T7895	punct	.,be
R4621	T7921	T7922	nsubj	Loss,led
R4622	T7923	T7921	prep	of,Loss
R4623	T7924	T7925	amod	functional,Dhcr7
R4624	T7925	T7923	pobj	Dhcr7,of
R4625	T7926	T7922	prep	to,led
R4626	T7927	T7928	det	a,retardation
R4627	T7928	T7926	pobj	retardation,to
R4628	T7929	T7928	prep	of,retardation
R4629	T7930	T7931	amod	embryonic,growth
R463	T1232	T1221	advcl	be,remains
R4630	T7931	T7929	pobj	growth,of
R4631	T7932	T7928	prep	from,retardation
R4632	T7933	T7932	pobj	E14.5,from
R4633	T7934	T7935	punct	~,E16.5
R4634	T7935	T7933	prep	E16.5,E14.5
R4635	T7936	T7933	advmod	onwards,E14.5
R4636	T7937	T7922	punct	.,led
R4637	T7939	T7940	prep	By,were
R4638	T7941	T7942	det	this,stage
R4639	T7942	T7939	pobj	stage,By
R464	T1233	T1234	poss	its,link
R4640	T7943	T7940	punct	", ",were
R4641	T7944	T7945	compound	cholesterol,levels
R4642	T7945	T7940	nsubj	levels,were
R4643	T7946	T7940	advmod	already,were
R4644	T7947	T7940	acomp	lower,were
R4645	T7948	T7940	cc	and,were
R4646	T7949	T7950	poss	its,precursor
R4647	T7950	T7952	nsubj	precursor,elevated
R4648	T7951	T7950	amod	immediate,precursor
R4649	T7952	T7940	conj	elevated,were
R465	T1234	T1232	nsubj	link,be
R4650	T7953	T7950	punct	", ",precursor
R4651	T7954	T7955	nummod	7,DHC
R4652	T7955	T7950	appos	DHC,precursor
R4653	T7956	T7955	punct	-,DHC
R4654	T7957	T7952	punct	", ",elevated
R4655	T7958	T7940	prep	in,were
R4656	T7959	T7960	det	the,lungs
R4657	T7960	T7958	pobj	lungs,in
R4658	T7961	T7962	punct	(,study
R4659	T7962	T7960	parataxis	study,lungs
R466	T1235	T1234	prep	with,link
R4660	T7963	T7962	det	this,study
R4661	T7964	T7962	punct	),study
R4662	T7965	T7966	advmod	as,as
R4663	T7966	T7958	cc	as,in
R4664	T7967	T7966	advmod	well,as
R4665	T7968	T7958	conj	in,in
R4666	T7969	T7970	det	the,body
R4667	T7970	T7968	pobj	body,in
R4668	T7971	T7970	amod	whole,body
R4669	T7972	T7973	punct	[,17
R467	T1236	T1235	pobj	microdomains,with
R4670	T7973	T7970	parataxis	17,body
R4671	T7974	T7973	nummod	16,17
R4672	T7975	T7973	punct	",",17
R4673	T7976	T7973	punct	],17
R4674	T7977	T7940	punct	.,were
R4675	T7979	T7980	amod	Intrauterine,retardation
R4676	T7980	T7982	nsubj	retardation,is
R4677	T7981	T7980	compound	growth,retardation
R4678	T7983	T7980	punct	(,retardation
R4679	T7984	T7980	appos	IUGR,retardation
R468	T1237	T1236	punct	(,microdomains
R4680	T7985	T7980	punct	),retardation
R4681	T7986	T7980	acl	followed,retardation
R4682	T7987	T7986	agent	by,followed
R4683	T7988	T7989	amod	postnatal,failure
R4684	T7989	T7987	pobj	failure,by
R4685	T7990	T7991	aux	to,thrive
R4686	T7991	T7989	acl	thrive,failure
R4687	T7992	T7993	det	a,phenotype
R4688	T7993	T7982	attr	phenotype,is
R4689	T7994	T7995	advmod	universally,observed
R469	T1238	T1239	advmod	variously,termed
R4690	T7995	T7993	amod	observed,phenotype
R4691	T7996	T7982	prep	in,is
R4692	T7997	T7998	compound	SLOS,patients
R4693	T7998	T7996	pobj	patients,in
R4694	T7999	T8000	punct	[,18
R4695	T8000	T7982	parataxis	18,is
R4696	T8001	T8000	nummod	2,18
R4697	T8002	T8000	punct	",",18
R4698	T8003	T8000	punct	],18
R4699	T8004	T7982	punct	.,is
R47	T292	T290	prep	of,disease
R470	T1239	T1236	acl	termed,microdomains
R4700	T8006	T8007	nsubj	We,showed
R4701	T8008	T8007	advmod	here,showed
R4702	T8009	T8010	mark	that,reduced
R4703	T8010	T8007	ccomp	reduced,showed
R4704	T8011	T8012	compound	body,length
R4705	T8012	T8010	nsubjpass	length,reduced
R4706	T8013	T8010	auxpass	was,reduced
R4707	T8014	T8010	advmod	significantly,reduced
R4708	T8015	T8010	prep	at,reduced
R4709	T8016	T8017	amod	later,stages
R471	T1240	T1239	oprd	rafts,termed
R4710	T8017	T8015	pobj	stages,at
R4711	T8018	T8017	prep	of,stages
R4712	T8019	T8018	pobj	gestation,of
R4713	T8020	T8010	prep	in,reduced
R4714	T8021	T8022	nmod	Dhcr7,animals
R4715	T8022	T8020	pobj	animals,in
R4716	T8023	T8021	punct	-,Dhcr7
R4717	T8024	T8021	punct	/,Dhcr7
R4718	T8025	T8021	punct	-,Dhcr7
R4719	T8026	T8010	punct	", ",reduced
R472	T1241	T1240	punct	", ",rafts
R4720	T8027	T8010	prep	compared,reduced
R4721	T8028	T8027	prep	to,compared
R4722	T8029	T8028	pobj	controls,to
R4723	T8030	T8007	punct	.,showed
R4724	T8032	T8033	amod	Whole,body
R4725	T8033	T8034	compound	body,weights
R4726	T8034	T8035	nsubj	weights,were
R4727	T8036	T8034	cc	and,weights
R4728	T8037	T8038	amod	fresh,lung
R4729	T8038	T8039	compound	lung,weights
R473	T1242	T1240	conj	caveoli,rafts
R4730	T8039	T8034	conj	weights,weights
R4731	T8040	T8034	punct	(,weights
R4732	T8041	T8042	advmod	as,as
R4733	T8042	T8034	cc	as,weights
R4734	T8043	T8042	advmod	well,as
R4735	T8044	T8034	conj	brain,weights
R4736	T8045	T8044	punct	", ",brain
R4737	T8046	T8044	conj	heart,brain
R4738	T8047	T8046	cc	and,heart
R4739	T8048	T8046	conj	kidney,heart
R474	T1243	T1242	advmod	etc.,caveoli
R4740	T8049	T8050	punct	", ",shown
R4741	T8050	T8048	parataxis	shown,kidney
R4742	T8051	T8050	nsubj	data,shown
R4743	T8052	T8050	neg	not,shown
R4744	T8053	T8050	punct	),shown
R4745	T8054	T8034	prep	at,weights
R4746	T8055	T8056	amod	late,stages
R4747	T8056	T8054	pobj	stages,at
R4748	T8057	T8056	amod	gestational,stages
R4749	T8058	T8035	advmod	also,were
R475	T1244	T1236	punct	),microdomains
R4750	T8059	T8035	acomp	lower,were
R4751	T8060	T8035	prep	in,were
R4752	T8061	T8062	nmod	Dhcr7,embryos
R4753	T8062	T8060	pobj	embryos,in
R4754	T8063	T8061	punct	-,Dhcr7
R4755	T8064	T8061	punct	/,Dhcr7
R4756	T8065	T8061	punct	-,Dhcr7
R4757	T8066	T8035	punct	.,were
R4758	T8068	T8069	advmod	Interestingly,suggested
R4759	T8070	T8069	punct	", ",suggested
R476	T1245	T1232	aux	may,be
R4760	T8071	T8072	det	the,ratios
R4761	T8072	T8069	nsubj	ratios,suggested
R4762	T8073	T8072	amod	lower,ratios
R4763	T8074	T8072	nmod	lung,ratios
R4764	T8075	T8076	punct	/,body
R4765	T8076	T8074	prep	body,lung
R4766	T8077	T8072	prep	at,ratios
R4767	T8078	T8077	pobj	E18.5,at
R4768	T8079	T8072	prep	in,ratios
R4769	T8080	T8081	nmod	Dhcr7,embryos
R477	T1246	T1232	attr	part,be
R4770	T8081	T8079	pobj	embryos,in
R4771	T8082	T8080	punct	-,Dhcr7
R4772	T8083	T8080	punct	/,Dhcr7
R4773	T8084	T8080	punct	-,Dhcr7
R4774	T8085	T8081	prep	compared,embryos
R4775	T8086	T8085	prep	to,compared
R4776	T8087	T8088	amod	wild,type
R4777	T8088	T8086	pobj	type,to
R4778	T8089	T8088	punct	-,type
R4779	T8090	T8091	det	a,inhibition
R478	T1247	T1246	prep	of,part
R4780	T8091	T8069	dobj	inhibition,suggested
R4781	T8092	T8091	amod	disproportionate,inhibition
R4782	T8093	T8091	prep	of,inhibition
R4783	T8094	T8095	compound	lung,growth
R4784	T8095	T8093	pobj	growth,of
R4785	T8096	T8091	prep	than,inhibition
R4786	T8097	T8098	amod	other,organs
R4787	T8098	T8096	pobj	organs,than
R4788	T8099	T8069	punct	.,suggested
R4789	T8101	T8102	compound	Cell,proliferation
R479	T1248	T1249	det	the,answer
R4790	T8102	T8103	nsubjpass	proliferation,arrested
R4791	T8104	T8102	cc	and,proliferation
R4792	T8105	T8102	conj	division,proliferation
R4793	T8106	T8102	prep	in,proliferation
R4794	T8107	T8108	nmod	Dhcr7,lungs
R4795	T8108	T8106	pobj	lungs,in
R4796	T8109	T8107	punct	-,Dhcr7
R4797	T8110	T8107	punct	/,Dhcr7
R4798	T8111	T8107	punct	-,Dhcr7
R4799	T8112	T8103	auxpass	were,arrested
R48	T293	T294	compound	Smith,Opitz
R480	T1249	T1247	pobj	answer,of
R4800	T8113	T8103	advmod	markedly,arrested
R4801	T8114	T8103	prep	without,arrested
R4802	T8115	T8116	amod	apparent,increase
R4803	T8116	T8114	pobj	increase,without
R4804	T8117	T8116	prep	in,increase
R4805	T8118	T8119	compound	cell,apoptosis
R4806	T8119	T8117	pobj	apoptosis,in
R4807	T8120	T8103	punct	.,arrested
R4808	T8122	T8123	advmod	Therefore,appears
R4809	T8124	T8123	punct	", ",appears
R481	T1250	T1251	punct	[,1
R4810	T8125	T8123	nsubj	it,appears
R4811	T8126	T8127	mark	that,was
R4812	T8127	T8123	ccomp	was,appears
R4813	T8128	T8129	compound	growth,retardation
R4814	T8129	T8127	nsubj	retardation,was
R4815	T8130	T8129	acl	caused,retardation
R4816	T8131	T8130	agent	by,caused
R4817	T8132	T8133	compound	cholesterol,deficiency
R4818	T8133	T8131	pobj	deficiency,by
R4819	T8134	T8135	advmod	primarily,because
R482	T1251	T1232	parataxis	1,be
R4820	T8135	T8127	prep	because,was
R4821	T8136	T8135	pcomp	of,because
R4822	T8137	T8138	det	an,inhibition
R4823	T8138	T8135	pobj	inhibition,because
R4824	T8139	T8138	prep	of,inhibition
R4825	T8140	T8141	compound	cell,growth
R4826	T8141	T8139	pobj	growth,of
R4827	T8142	T8141	cc	and,growth
R4828	T8143	T8141	conj	proliferation,growth
R4829	T8144	T8138	punct	", ",inhibition
R483	T1252	T1251	punct	],1
R4830	T8145	T8146	advmod	rather,than
R4831	T8146	T8138	prep	than,inhibition
R4832	T8147	T8148	det	an,increase
R4833	T8148	T8146	pobj	increase,than
R4834	T8149	T8148	prep	in,increase
R4835	T8150	T8151	compound	cell,death
R4836	T8151	T8149	pobj	death,in
R4837	T8152	T8123	punct	.,appears
R4838	T8154	T8155	nsubj	Cholesterol,is
R4839	T8156	T8155	acomp	present,is
R484	T1253	T1221	punct	.,remains
R4840	T8157	T8155	prep	in,is
R4841	T8158	T8159	nmod	Dhcr7,embryos
R4842	T8159	T8157	pobj	embryos,in
R4843	T8160	T8158	punct	-,Dhcr7
R4844	T8161	T8158	punct	/,Dhcr7
R4845	T8162	T8158	punct	-,Dhcr7
R4846	T8163	T8155	punct	", ",is
R4847	T8164	T8165	mark	as,is
R4848	T8165	T8155	advcl	is,is
R4849	T8166	T8165	nsubj	it,is
R485	T1255	T1256	mark	Although,led
R4850	T8167	T8165	prep	in,is
R4851	T8168	T8169	nmod	Sc5d,embryos
R4852	T8169	T8167	pobj	embryos,in
R4853	T8170	T8168	punct	-,Sc5d
R4854	T8171	T8168	punct	/,Sc5d
R4855	T8172	T8168	punct	-,Sc5d
R4856	T8173	T8174	punct	(,lathosterolosis
R4857	T8174	T8168	parataxis	lathosterolosis,Sc5d
R4858	T8175	T8174	punct	),lathosterolosis
R4859	T8176	T8168	cc	and,Sc5d
R486	T1256	T1265	advcl	led,proven
R4860	T8177	T8168	conj	Dhcr24,Sc5d
R4861	T8178	T8177	punct	-,Dhcr24
R4862	T8179	T8177	punct	/,Dhcr24
R4863	T8180	T8177	punct	-,Dhcr24
R4864	T8181	T8182	punct	(,desmosterolosis
R4865	T8182	T8177	parataxis	desmosterolosis,Dhcr24
R4866	T8183	T8182	punct	),desmosterolosis
R4867	T8184	T8185	punct	[,38
R4868	T8185	T8155	parataxis	38,is
R4869	T8186	T8185	nummod	15,38
R487	T1257	T1258	det	the,accumulation
R4870	T8187	T8185	punct	",",38
R4871	T8188	T8185	punct	],38
R4872	T8189	T8155	punct	.,is
R4873	T8191	T8192	amod	Total,sterols
R4874	T8192	T8193	nsubjpass	sterols,reduced
R4875	T8194	T8192	cc	and,sterols
R4876	T8195	T8192	conj	cholesterol,sterols
R4877	T8196	T8192	prep	in,sterols
R4878	T8197	T8198	nmod	Dhcr7,lungs
R4879	T8198	T8196	pobj	lungs,in
R488	T1258	T1256	nsubj	accumulation,led
R4880	T8199	T8197	punct	-,Dhcr7
R4881	T8200	T8197	punct	/,Dhcr7
R4882	T8201	T8197	punct	-,Dhcr7
R4883	T8202	T8198	amod	embryonic,lungs
R4884	T8203	T8196	punct	(,in
R4885	T8204	T8205	advmod	as,as
R4886	T8205	T8196	cc	as,in
R4887	T8206	T8205	advmod	well,as
R4888	T8207	T8196	conj	in,in
R4889	T8208	T8207	pobj	brain,in
R489	T1259	T1258	prep	of,accumulation
R4890	T8209	T8208	cc	and,brain
R4891	T8210	T8208	conj	liver,brain
R4892	T8211	T8212	punct	", ",shown
R4893	T8212	T8192	parataxis	shown,sterols
R4894	T8213	T8212	nsubj	data,shown
R4895	T8214	T8212	neg	not,shown
R4896	T8215	T8212	punct	),shown
R4897	T8216	T8193	auxpass	were,reduced
R4898	T8217	T8193	cc	and,reduced
R4899	T8218	T8219	nmod	7DHC,8DHC
R49	T294	T298	compound	Opitz,syndrome
R490	T1260	T1259	pobj	cholesterol,of
R4900	T8219	T8221	nsubj	8DHC,elevated
R4901	T8220	T8219	punct	/,8DHC
R4902	T8221	T8193	conj	elevated,reduced
R4903	T8222	T8193	punct	", ",reduced
R4904	T8223	T8193	cc	but,reduced
R4905	T8224	T8225	det	a,amount
R4906	T8225	T8227	nsubj	amount,was
R4907	T8226	T8225	amod	significant,amount
R4908	T8227	T8193	conj	was,reduced
R4909	T8228	T8225	prep	of,amount
R491	T1261	T1258	prep	in,accumulation
R4910	T8229	T8228	pobj	cholesterol,of
R4911	T8230	T8227	acomp	detectable,was
R4912	T8231	T8227	punct	", ",was
R4913	T8232	T8227	advcl	comprising,was
R4914	T8233	T8234	punct	~,60
R4915	T8234	T8235	nummod	60,%
R4916	T8235	T8232	dobj	%,comprising
R4917	T8236	T8235	cc	and,%
R4918	T8237	T8238	punct	~,30
R4919	T8238	T8239	nummod	30,%
R492	T1262	T1263	amod	atherosclerotic,plaques
R4920	T8239	T8235	conj	%,%
R4921	T8240	T8235	prep	of,%
R4922	T8241	T8242	amod	total,sterols
R4923	T8242	T8240	pobj	sterols,of
R4924	T8243	T8242	compound	tissue,sterols
R4925	T8244	T8232	prep	at,comprising
R4926	T8245	T8244	pobj	E13.5,at
R4927	T8246	T8245	cc	and,E13.5
R4928	T8247	T8245	conj	neonate,E13.5
R4929	T8248	T8232	punct	", ",comprising
R493	T1263	T1261	pobj	plaques,in
R4930	T8249	T8232	advmod	respectively,comprising
R4931	T8250	T8227	punct	.,was
R4932	T8252	T8253	amod	Fetal,cholesterol
R4933	T8253	T8254	nsubjpass	cholesterol,synthesized
R4934	T8255	T8254	aux	can,synthesized
R4935	T8256	T8254	preconj	either,synthesized
R4936	T8257	T8254	auxpass	be,synthesized
R4937	T8258	T8254	advmod	endogenously,synthesized
R4938	T8259	T8254	prep	in,synthesized
R4939	T8260	T8261	amod	fetal,tissues
R494	T1264	T1256	aux	has,led
R4940	T8261	T8259	pobj	tissues,in
R4941	T8262	T8254	cc	or,synthesized
R4942	T8263	T8254	conj	accrued,synthesized
R4943	T8264	T8263	prep	from,accrued
R4944	T8265	T8266	amod	extra-embryonic,tissues
R4945	T8266	T8264	pobj	tissues,from
R4946	T8267	T8268	amod	such,as
R4947	T8268	T8266	prep	as,tissues
R4948	T8269	T8270	amod	maternal,serum
R4949	T8270	T8268	pobj	serum,as
R495	T1266	T1256	prep	to,led
R4950	T8271	T8270	punct	", ",serum
R4951	T8272	T8270	conj	placenta,serum
R4952	T8273	T8272	punct	", ",placenta
R4953	T8274	T8272	cc	and,placenta
R4954	T8275	T8276	compound	yolk,sac
R4955	T8276	T8272	conj	sac,placenta
R4956	T8277	T8278	punct	[,39
R4957	T8278	T8254	parataxis	39,synthesized
R4958	T8279	T8278	punct	],39
R4959	T8280	T8254	punct	.,synthesized
R496	T1267	T1268	det	the,concept
R4960	T8282	T8283	mark	Since,synthesize
R4961	T8283	T8289	advcl	synthesize,derived
R4962	T8284	T8285	det	these,mice
R4963	T8285	T8283	nsubj	mice,synthesize
R4964	T8286	T8283	advmod	theoretically,synthesize
R4965	T8287	T8283	aux	can,synthesize
R4966	T8288	T8283	neg	not,synthesize
R4967	T8290	T8283	dobj	cholesterol,synthesize
R4968	T8291	T8289	punct	", ",derived
R4969	T8292	T8293	det	any,accumulation
R497	T1268	T1266	pobj	concept,to
R4970	T8293	T8289	nsubjpass	accumulation,derived
R4971	T8294	T8293	prep	of,accumulation
R4972	T8295	T8294	pobj	cholesterol,of
R4973	T8296	T8289	aux	must,derived
R4974	T8297	T8289	auxpass	be,derived
R4975	T8298	T8289	prep	from,derived
R4976	T8299	T8300	det	the,sources
R4977	T8300	T8298	pobj	sources,from
R4978	T8301	T8300	amod	maternal,sources
R4979	T8302	T8289	punct	.,derived
R498	T1269	T1270	mark	that,be
R4980	T8304	T8305	advmod	Interestingly,are
R4981	T8306	T8305	punct	", ",are
R4982	T8307	T8308	npadvmod	Dhcr24,null
R4983	T8308	T8309	amod	null,mice
R4984	T8309	T8305	nsubj	mice,are
R4985	T8310	T8305	acomp	viable,are
R4986	T8311	T8305	cc	but,are
R4987	T8312	T8305	conj	contained,are
R4988	T8313	T8314	advmod	almost,cholesterol
R4989	T8314	T8312	dobj	cholesterol,contained
R499	T1270	T1268	acl	be,concept
R4990	T8315	T8314	det	no,cholesterol
R4991	T8316	T8312	prep	in,contained
R4992	T8317	T8316	pobj	plasma,in
R4993	T8318	T8317	cc	and,plasma
R4994	T8319	T8317	conj	tissues,plasma
R4995	T8320	T8312	prep	at,contained
R4996	T8321	T8322	nummod	3,months
R4997	T8322	T8320	pobj	months,at
R4998	T8323	T8322	punct	-,months
R4999	T8324	T8305	punct	", ",are
R5	T241	T243	amod	gestational,hypoplasia
R50	T295	T294	punct	-,Opitz
R500	T1271	T1272	punct	',much
R5000	T8325	T8326	mark	whereas,accounts
R5001	T8326	T8305	advcl	accounts,are
R5002	T8327	T8328	compound	cholesterol,content
R5003	T8328	T8326	nsubj	content,accounts
R5004	T8329	T8328	prep	in,content
R5005	T8330	T8331	npadvmod	Dhcr24,null
R5006	T8331	T8332	amod	null,tissues
R5007	T8332	T8329	pobj	tissues,in
R5008	T8333	T8332	compound	embryo,tissues
R5009	T8334	T8326	prep	for,accounts
R501	T1272	T1274	amod	much,cholesterol
R5010	T8335	T8336	punct	~,60
R5011	T8336	T8337	nummod	60,%
R5012	T8337	T8334	pobj	%,for
R5013	T8338	T8337	prep	at,%
R5014	T8339	T8338	pobj	E11.5,at
R5015	T8340	T8337	cc	and,%
R5016	T8341	T8342	punct	~,30
R5017	T8342	T8343	nummod	30,%
R5018	T8343	T8337	conj	%,%
R5019	T8344	T8343	prep	at,%
R502	T1273	T1272	advmod	too,much
R5020	T8345	T8344	pobj	E17.5,at
R5021	T8346	T8337	punct	", ",%
R5022	T8347	T8337	prep	of,%
R5023	T8348	T8349	amod	total,sterols
R5024	T8349	T8347	pobj	sterols,of
R5025	T8350	T8349	compound	tissue,sterols
R5026	T8351	T8352	punct	[,38
R5027	T8352	T8326	parataxis	38,accounts
R5028	T8353	T8352	punct	],38
R5029	T8354	T8305	punct	.,are
R503	T1274	T1270	nsubj	cholesterol,be
R5030	T8356	T8357	det	These,results
R5031	T8357	T8358	nsubj	results,suggest
R5032	T8359	T8357	punct	", ",results
R5033	T8360	T8361	advmod	as,as
R5034	T8361	T8357	cc	as,results
R5035	T8362	T8361	advmod	well,as
R5036	T8363	T8364	det	this,study
R5037	T8364	T8357	conj	study,results
R5038	T8365	T8358	punct	", ",suggest
R5039	T8366	T8367	mark	that,transferred
R504	T1275	T1272	punct	',much
R5040	T8367	T8358	ccomp	transferred,suggest
R5041	T8368	T8369	det	a,amount
R5042	T8369	T8367	nsubjpass	amount,transferred
R5043	T8370	T8369	amod	considerable,amount
R5044	T8371	T8369	prep	of,amount
R5045	T8372	T8373	amod	maternal,cholesterol
R5046	T8373	T8371	pobj	cholesterol,of
R5047	T8374	T8367	aux	can,transferred
R5048	T8375	T8367	auxpass	be,transferred
R5049	T8376	T8367	prep	to,transferred
R505	T1276	T1270	aux	may,be
R5050	T8377	T8378	det	the,fetus
R5051	T8378	T8376	pobj	fetus,to
R5052	T8379	T8378	amod	murine,fetus
R5053	T8380	T8358	punct	.,suggest
R5054	T8382	T8383	nummod	One,explanation
R5055	T8383	T8384	nsubj	explanation,be
R5056	T8385	T8383	prep	of,explanation
R5057	T8386	T8387	advmod	why,are
R5058	T8387	T8385	pcomp	are,of
R5059	T8388	T8389	det	the,mice
R506	T1277	T1270	acomp	deleterious,be
R5060	T8389	T8387	nsubj	mice,are
R5061	T8390	T8389	nmod	Dhcr24,mice
R5062	T8391	T8390	punct	-,Dhcr24
R5063	T8392	T8390	punct	/,Dhcr24
R5064	T8393	T8390	punct	-,Dhcr24
R5065	T8394	T8395	advmod	developmentally,normal
R5066	T8395	T8387	acomp	normal,are
R5067	T8396	T8387	cc	but,are
R5068	T8397	T8398	nmod	Dhcr7,mice
R5069	T8398	T8402	nsubjpass	mice,affected
R507	T1278	T1265	punct	", ",proven
R5070	T8399	T8397	punct	-,Dhcr7
R5071	T8400	T8397	punct	/,Dhcr7
R5072	T8401	T8397	punct	-,Dhcr7
R5073	T8402	T8387	conj	affected,are
R5074	T8403	T8402	auxpass	are,affected
R5075	T8404	T8384	aux	may,be
R5076	T8405	T8406	mark	because,substitute
R5077	T8406	T8384	advcl	substitute,be
R5078	T8407	T8406	nsubj	desmosterol,substitute
R5079	T8408	T8406	aux	can,substitute
R508	T1279	T1280	advmod	too,cholesterol
R5080	T8409	T8406	advmod	functionally,substitute
R5081	T8410	T8406	prep	for,substitute
R5082	T8411	T8410	pobj	cholesterol,for
R5083	T8412	T8406	punct	", ",substitute
R5084	T8413	T8414	mark	whereas,can
R5085	T8414	T8406	advcl	can,substitute
R5086	T8415	T8416	det	the,precursors
R5087	T8416	T8414	nsubj	precursors,can
R5088	T8417	T8416	amod	other,precursors
R5089	T8418	T8416	compound	cholesterol,precursors
R509	T1280	T1265	nsubjpass	cholesterol,proven
R5090	T8419	T8414	neg	not,can
R5091	T8420	T8384	punct	.,be
R5092	T8422	T8423	mark	Although,lacking
R5093	T8423	T8440	advcl	lacking,been
R5094	T8424	T8425	det	a,understanding
R5095	T8425	T8423	nsubj	understanding,lacking
R5096	T8426	T8425	amod	mechanistic,understanding
R5097	T8427	T8425	prep	of,understanding
R5098	T8428	T8429	advmod	why,leads
R5099	T8429	T8427	pcomp	leads,of
R51	T296	T294	compound	Lemli,Opitz
R510	T1281	T1280	amod	little,cholesterol
R5100	T8430	T8431	det	a,deficiency
R5101	T8431	T8429	nsubj	deficiency,leads
R5102	T8432	T8431	prep	in,deficiency
R5103	T8433	T8434	compound	cholesterol,biosynthesis
R5104	T8434	T8432	pobj	biosynthesis,in
R5105	T8435	T8429	prep	to,leads
R5106	T8436	T8437	amod	abnormal,development
R5107	T8437	T8435	pobj	development,to
R5108	T8438	T8437	amod	embryonic,development
R5109	T8439	T8423	aux	is,lacking
R511	T1282	T1265	aux	has,proven
R5110	T8441	T8440	punct	", ",been
R5111	T8442	T8443	det	a,explanation
R5112	T8443	T8440	nsubj	explanation,been
R5113	T8444	T8445	advmod	frequently,advanced
R5114	T8445	T8443	amod	advanced,explanation
R5115	T8446	T8440	aux	has,been
R5116	T8447	T8448	mark	that,disrupted
R5117	T8448	T8440	ccomp	disrupted,been
R5118	T8449	T8450	compound	Shh,signaling
R5119	T8450	T8448	nsubjpass	signaling,disrupted
R512	T1283	T1265	advmod	now,proven
R5120	T8451	T8450	punct	", ",signaling
R5121	T8452	T8450	acl	involved,signaling
R5122	T8453	T8452	prep	in,involved
R5123	T8454	T8455	amod	early,patterning
R5124	T8455	T8453	pobj	patterning,in
R5125	T8456	T8455	amod	developmental,patterning
R5126	T8457	T8448	punct	", ",disrupted
R5127	T8458	T8448	aux	may,disrupted
R5128	T8459	T8448	auxpass	be,disrupted
R5129	T8460	T8440	punct	.,been
R513	T1284	T1265	auxpass	been,proven
R5130	T8462	T8463	prep	In,expressed
R5131	T8464	T8465	det	the,primordium
R5132	T8465	T8462	pobj	primordium,In
R5133	T8466	T8465	amod	developing,primordium
R5134	T8467	T8465	compound	lung,primordium
R5135	T8468	T8463	punct	", ",expressed
R5136	T8469	T8463	nsubjpass	Shh,expressed
R5137	T8470	T8463	auxpass	is,expressed
R5138	T8471	T8463	advmod	initially,expressed
R5139	T8472	T8463	prep	at,expressed
R514	T1285	T1265	oprd	pathophysiological,proven
R5140	T8473	T8472	pobj	E9,at
R5141	T8474	T8463	cc	and,expressed
R5142	T8475	T8463	conj	promotes,expressed
R5143	T8476	T8477	amod	branching,morphogenesis
R5144	T8477	T8475	dobj	morphogenesis,promotes
R5145	T8478	T8477	punct	", ",morphogenesis
R5146	T8479	T8480	dep	which,impaired
R5147	T8480	T8477	relcl	impaired,morphogenesis
R5148	T8481	T8480	auxpass	is,impaired
R5149	T8482	T8480	prep	in,impaired
R515	T1286	T1265	prep	for,proven
R5150	T8483	T8484	compound	Shh,knockout
R5151	T8484	T8485	compound	knockout,mice
R5152	T8485	T8482	pobj	mice,in
R5153	T8486	T8487	punct	[,24
R5154	T8487	T8480	parataxis	24,impaired
R5155	T8488	T8487	punct	],24
R5156	T8489	T8463	punct	", ",expressed
R5157	T8490	T8463	advcl	suggesting,expressed
R5158	T8491	T8492	mark	that,is
R5159	T8492	T8490	ccomp	is,suggesting
R516	T1287	T1288	amod	abnormal,development
R5160	T8493	T8492	nsubj	Shh,is
R5161	T8494	T8493	punct	", ",Shh
R5162	T8495	T8493	acl	secreted,Shh
R5163	T8496	T8495	agent	by,secreted
R5164	T8497	T8498	det	the,epithelium
R5165	T8498	T8496	pobj	epithelium,by
R5166	T8499	T8492	punct	", ",is
R5167	T8500	T8492	acomp	critical,is
R5168	T8501	T8500	prep	for,critical
R5169	T8502	T8503	amod	branching,morphogenesis
R517	T1288	T1286	pobj	development,for
R5170	T8503	T8501	pobj	morphogenesis,for
R5171	T8504	T8463	punct	.,expressed
R5172	T8506	T8507	prep	In,observed
R5173	T8508	T8509	det	this,study
R5174	T8509	T8506	pobj	study,In
R5175	T8510	T8507	punct	", ",observed
R5176	T8511	T8512	det	the,lack
R5177	T8512	T8507	nsubjpass	lack,observed
R5178	T8513	T8512	prep	of,lack
R5179	T8514	T8513	pobj	evidence,of
R518	T1289	T1288	amod	embryonic,development
R5180	T8515	T8514	prep	of,evidence
R5181	T8516	T8517	amod	abnormal,morphogenesis
R5182	T8517	T8515	pobj	morphogenesis,of
R5183	T8518	T8517	nmod	lung,morphogenesis
R5184	T8519	T8517	amod	branching,morphogenesis
R5185	T8520	T8514	prep	in,evidence
R5186	T8521	T8522	det	the,stages
R5187	T8522	T8520	pobj	stages,in
R5188	T8523	T8522	amod	early,stages
R5189	T8524	T8512	punct	", ",lack
R519	T1290	T1265	punct	.,proven
R5190	T8525	T8512	cc	but,lack
R5191	T8526	T8527	det	a,maturation
R5192	T8527	T8512	conj	maturation,lack
R5193	T8528	T8527	amod	consistent,maturation
R5194	T8529	T8527	amod	delayed,maturation
R5195	T8530	T8527	prep	of,maturation
R5196	T8531	T8532	det	the,region
R5197	T8532	T8530	pobj	region,of
R5198	T8533	T8534	compound	gas,exchange
R5199	T8534	T8532	compound	exchange,region
R52	T297	T294	punct	-,Opitz
R520	T1292	T1293	compound	Smith,Opitz
R5200	T8535	T8534	punct	-,exchange
R5201	T8536	T8532	prep	of,region
R5202	T8537	T8538	det	the,lung
R5203	T8538	T8536	pobj	lung,of
R5204	T8539	T8527	prep	in,maturation
R5205	T8540	T8541	det	the,period
R5206	T8541	T8539	pobj	period,in
R5207	T8542	T8541	amod	saccular,period
R5208	T8543	T8541	prep	of,period
R5209	T8544	T8543	pobj	development,of
R521	T1293	T1297	compound	Opitz,syndrome
R5210	T8545	T8544	prep	of,development
R5211	T8546	T8547	nmod	Dhcr7,embryos
R5212	T8547	T8545	pobj	embryos,of
R5213	T8548	T8546	punct	-,Dhcr7
R5214	T8549	T8546	punct	/,Dhcr7
R5215	T8550	T8546	punct	-,Dhcr7
R5216	T8551	T8507	auxpass	was,observed
R5217	T8552	T8507	punct	", ",observed
R5218	T8553	T8507	advcl	suggesting,observed
R5219	T8554	T8555	amod	early,signaling
R522	T1294	T1293	punct	-,Opitz
R5220	T8555	T8557	nsubj	signaling,was
R5221	T8556	T8555	compound	Shh,signaling
R5222	T8557	T8553	advcl	was,suggesting
R5223	T8558	T8557	acomp	normal,was
R5224	T8559	T8507	punct	.,observed
R5225	T8561	T8562	compound	Shh,expression
R5226	T8562	T8564	nsubj	expression,was
R5227	T8563	T8562	compound	protein,expression
R5228	T8565	T8562	cc	and,expression
R5229	T8602	T8603	advmod	Thus,is
R523	T1295	T1293	compound	Lemli,Opitz
R5230	T8566	T8562	conj	autoprocessing,expression
R5231	T8604	T8603	punct	", ",is
R5232	T8605	T8603	nsubj	it,is
R5233	T8567	T8562	prep	at,expression
R5234	T8606	T8603	acomp	possible,is
R5235	T8607	T8608	mark	that,were
R5236	T8568	T8567	pobj	E11.5,at
R5237	T8569	T8564	acomp	indistinguishable,was
R5238	T8608	T8603	ccomp	were,is
R5239	T8570	T8564	prep	between,was
R524	T1296	T1293	punct	-,Opitz
R5240	T8609	T8610	det	the,levels
R5241	T8610	T8608	nsubj	levels,were
R5242	T8611	T8610	compound	cholesterol,levels
R5243	T8571	T8572	amod	wild,type
R5244	T8612	T8613	punct	(,%
R5245	T8613	T8610	parataxis	%,levels
R5246	T8572	T8574	nmod	type,embryos
R5247	T8614	T8615	advmod	roughly,50
R5248	T8615	T8613	nummod	50,%
R5249	T8616	T8613	prep	of,%
R525	T1297	T1298	nsubjpass	syndrome,caused
R5250	T8617	T8618	amod	normal,levels
R5251	T8573	T8572	punct	-,type
R5252	T8618	T8616	pobj	levels,of
R5253	T8619	T8613	punct	),%
R5254	T8574	T8570	pobj	embryos,between
R5255	T8620	T8610	prep	in,levels
R5256	T8621	T8622	nmod	Dhcr7,embryos
R5257	T8575	T8572	cc	and,type
R5258	T8622	T8620	pobj	embryos,in
R5259	T8623	T8621	punct	-,Dhcr7
R526	T1299	T1297	punct	(,syndrome
R5260	T8624	T8621	punct	/,Dhcr7
R5261	T8576	T8572	conj	Dhcr7,type
R5262	T8625	T8621	punct	-,Dhcr7
R5263	T8626	T8608	acomp	enough,were
R5264	T8627	T8626	prep	for,enough
R5265	T8628	T8627	pobj	completion,for
R5266	T8577	T8576	punct	-,Dhcr7
R5267	T8629	T8628	prep	of,completion
R5268	T8630	T8631	compound	Shh,autoprocessing
R5269	T8631	T8629	pobj	autoprocessing,of
R527	T1300	T1297	appos	SLOS,syndrome
R5270	T8578	T8576	punct	/,Dhcr7
R5271	T8632	T8608	cc	or,were
R5272	T8579	T8576	punct	-,Dhcr7
R5273	T8633	T8634	nsubj	7DHC,participate
R5274	T8634	T8608	conj	participate,were
R5275	T8635	T8633	acl	accumulated,7DHC
R5276	T8636	T8635	prep	in,accumulated
R5277	T8580	T8564	punct	.,was
R5278	T8637	T8638	nmod	Dhcr7,tissues
R5279	T8638	T8636	pobj	tissues,in
R528	T1301	T1302	punct	", ",MIM
R5280	T8639	T8637	punct	-,Dhcr7
R5281	T8582	T8583	advmod	Additionally,appeared
R5282	T8640	T8637	punct	/,Dhcr7
R5283	T8641	T8637	punct	-,Dhcr7
R5284	T8642	T8638	amod	embryonic,tissues
R5285	T8643	T8634	aux	can,participate
R5286	T8584	T8583	punct	", ",appeared
R5287	T8644	T8634	advmod	also,participate
R5288	T8645	T8634	advmod	efficiently,participate
R5289	T8646	T8634	prep	as,participate
R529	T1302	T1297	parataxis	MIM,syndrome
R5290	T8647	T8648	det	a,adduct
R5291	T8648	T8646	pobj	adduct,as
R5292	T8585	T8586	mark	although,were
R5293	T8649	T8648	compound	sterol,adduct
R5294	T8650	T8634	prep	in,participate
R5295	T8586	T8583	advcl	were,appeared
R5296	T8651	T8652	det	the,reaction
R5297	T8652	T8650	pobj	reaction,in
R5298	T8653	T8652	compound	Shh,reaction
R5299	T8587	T8588	det	these,experiments
R53	T298	T292	pobj	syndrome,of
R530	T1303	T1302	nummod	270400,MIM
R5300	T8654	T8652	compound	processing,reaction
R5301	T8655	T8656	punct	[,20
R5302	T8588	T8586	nsubj	experiments,were
R5303	T8656	T8634	parataxis	20,participate
R5304	T8657	T8656	punct	],20
R5305	T8658	T8603	punct	.,is
R5306	T8589	T8586	neg	not,were
R5307	T8660	T8661	prep	In,examined
R5308	T8590	T8586	acomp	quantitative,were
R5309	T8662	T8663	det	this,study
R531	T1304	T1302	punct	),MIM
R5310	T8663	T8660	pobj	study,In
R5311	T8591	T8583	punct	", ",appeared
R5312	T8664	T8661	punct	", ",examined
R5313	T8665	T8661	nsubj	we,examined
R5314	T8666	T8661	advmod	also,examined
R5315	T8667	T8668	det	the,pathway
R5316	T8668	T8661	dobj	pathway,examined
R5317	T8669	T8668	compound	Shh,pathway
R5318	T8592	T8593	det	the,abundance
R5319	T8670	T8668	compound	signaling,pathway
R532	T1305	T1297	punct	", ",syndrome
R5320	T8671	T8661	prep	by,examined
R5321	T8672	T8671	pobj	immunohistochemistry,by
R5322	T8593	T8583	nsubj	abundance,appeared
R5323	T8673	T8672	prep	of,immunohistochemistry
R5324	T8594	T8593	prep	of,abundance
R5325	T8674	T8675	amod	known,components
R5326	T8675	T8673	pobj	components,of
R5327	T8676	T8675	amod	key,components
R5328	T8677	T8675	prep	of,components
R5329	T8595	T8594	pobj	Shh,of
R533	T1306	T1307	det	a,disorder
R5330	T8678	T8679	det	this,pathway
R5331	T8679	T8677	pobj	pathway,of
R5332	T8680	T8675	punct	", ",components
R5333	T8596	T8583	oprd	unchanged,appeared
R5334	T8681	T8682	amod	such,as
R5335	T8682	T8675	prep	as,components
R5336	T8683	T8684	amod	patched,proteins
R5337	T8597	T8583	prep	between,appeared
R5338	T8684	T8682	pobj	proteins,as
R5339	T8685	T8683	punct	", ",patched
R534	T1307	T1297	appos	disorder,syndrome
R5340	T8598	T8599	det	these,genotypes
R5341	T8686	T8683	conj	smoothened,patched
R5342	T8687	T8686	cc	and,smoothened
R5343	T8688	T8686	conj	Gli,smoothened
R5344	T8599	T8597	pobj	genotypes,between
R5345	T8689	T8661	punct	.,examined
R5346	T8691	T8692	compound	Shh,signaling
R5347	T8600	T8583	punct	.,appeared
R5348	T8692	T8693	nsubj	signaling,leads
R5349	T8707	T8693	parataxis	40,leads
R535	T1308	T1309	amod	relative,common
R5350	T8694	T8693	prep	to,leads
R5351	T8695	T8696	det	the,activation
R5352	T8708	T8709	punct	-,42
R5353	T8696	T8694	pobj	activation,to
R5354	T8697	T8696	prep	of,activation
R5355	T8698	T8697	pobj	transcription,of
R5356	T8699	T8696	cc	and,activation
R5357	T8709	T8707	prep	42,40
R5358	T8700	T8701	amod	increased,levels
R5359	T8710	T8707	punct	],40
R536	T1309	T1307	amod	common,disorder
R5360	T8701	T8696	conj	levels,activation
R5361	T8702	T8701	compound	protein,levels
R5362	T8711	T8693	punct	.,leads
R5363	T8703	T8701	prep	of,levels
R5364	T8704	T8705	det	these,components
R5365	T8705	T8703	pobj	components,of
R5366	T8706	T8707	punct	[,40
R5367	T8713	T8714	mark	If,were
R5368	T8714	T8717	advcl	were,resulted
R5369	T8715	T8716	compound	Shh,signaling
R537	T1310	T1307	compound	dysmorphology,disorder
R5370	T8813	T8773	punct	.,showed
R5371	T8716	T8714	nsubj	signaling,were
R5372	T8815	T8816	advmod	However,was
R5373	T8718	T8714	acomp	defective,were
R5374	T8817	T8816	punct	", ",was
R5375	T8818	T8819	det	a,deficiency
R5376	T8819	T8816	nsubj	deficiency,was
R5377	T8820	T8819	prep	in,deficiency
R5378	T8719	T8714	prep	in,were
R5379	T8821	T8822	poss	their,ability
R538	T1311	T1298	punct	", ",caused
R5380	T8822	T8820	pobj	ability,in
R5381	T8720	T8721	nmod	Dhcr7,embryos
R5382	T8823	T8824	aux	to,respond
R5383	T8824	T8822	acl	respond,ability
R5384	T8825	T8824	prep	to,respond
R5385	T8721	T8719	pobj	embryos,in
R5386	T8826	T8827	compound	Shh,activation
R5387	T8827	T8825	pobj	activation,to
R5388	T8828	T8816	punct	", ",was
R5389	T8722	T8720	punct	-,Dhcr7
R539	T1312	T1298	auxpass	is,caused
R5390	T8829	T8830	mark	as,judged
R5391	T8830	T8816	advcl	judged,was
R5392	T8831	T8830	prep	by,judged
R5393	T8723	T8720	punct	/,Dhcr7
R5394	T8832	T8833	compound	reporter,expression
R5395	T8833	T8831	pobj	expression,by
R5396	T8834	T8833	compound	gene,expression
R5397	T8835	T8816	punct	", ",was
R5398	T8724	T8720	punct	-,Dhcr7
R5399	T8836	T8816	acomp	demonstrable,was
R54	T299	T275	punct	", ",die
R540	T1313	T1298	agent	by,caused
R5400	T8837	T8816	punct	.,was
R5401	T8725	T8717	punct	", ",resulted
R5402	T8839	T8840	prep	In,was
R5403	T8726	T8717	nsubj	these,resulted
R5404	T8841	T8839	pobj	contrast,In
R5405	T8842	T8840	punct	", ",was
R5406	T8843	T8844	advmod	when,subjected
R5407	T8727	T8717	aux	could,resulted
R5408	T8844	T8840	advcl	subjected,was
R5409	T8845	T8846	compound	CHO,cells
R541	T1314	T1313	pobj	mutations,by
R5410	T8846	T8844	nsubjpass	cells,subjected
R5411	T8728	T8717	aux	have,resulted
R5412	T8847	T8846	punct	", ",cells
R5413	T8848	T8849	dep	that,expressed
R5414	T8849	T8846	relcl	expressed,cells
R5415	T8729	T8717	prep	in,resulted
R5416	T8850	T8849	advmod	stably,expressed
R5417	T8851	T8849	dobj	Shh,expressed
R5418	T8730	T8729	pobj	abnormalities,in
R5419	T8852	T8844	punct	", ",subjected
R542	T1315	T1314	prep	in,mutations
R5420	T8853	T8844	auxpass	were,subjected
R5421	T8854	T8844	prep	to,subjected
R5422	T8855	T8856	compound	sterol,deprivation
R5423	T8856	T8854	pobj	deprivation,to
R5424	T8857	T8840	punct	", ",was
R5425	T8731	T8730	prep	of,abnormalities
R5426	T8858	T8859	det	an,arrest
R5427	T8859	T8840	nsubj	arrest,was
R5428	T8860	T8859	prep	of,arrest
R5429	T8732	T8733	det	the,patterns
R543	T1316	T1315	pobj	DHCR7,in
R5430	T8861	T8862	compound	Shh,autoprocessing
R5431	T8862	T8860	pobj	autoprocessing,of
R5432	T8863	T8864	advmod	clearly,demonstrable
R5433	T8733	T8731	pobj	patterns,of
R5434	T8864	T8840	acomp	demonstrable,was
R5435	T8865	T8866	punct	[,29
R5436	T8866	T8840	parataxis	29,was
R5437	T8734	T8733	amod	staining,patterns
R5438	T8867	T8866	punct	],29
R5439	T8868	T8840	punct	.,was
R544	T1317	T1318	punct	[,2
R5440	T8735	T8733	prep	of,patterns
R5441	T8870	T8871	prep	In,detect
R5442	T8872	T8873	det	the,study
R5443	T8873	T8870	pobj	study,In
R5444	T8736	T8737	det	these,components
R5445	T8874	T8873	amod	present,study
R5446	T8875	T8871	punct	", ",detect
R5447	T8737	T8735	pobj	components,of
R5448	T8738	T8717	punct	", ",resulted
R5449	T8876	T8871	nsubj	we,detect
R545	T1318	T1316	parataxis	2,DHCR7
R5450	T8877	T8871	aux	did,detect
R5451	T8878	T8871	neg	not,detect
R5452	T8739	T8717	cc	but,resulted
R5453	T8879	T8880	amod	unprocessed,Shh
R5454	T8880	T8871	dobj	Shh,detect
R5455	T8881	T8871	prep	in,detect
R5456	T8740	T8741	det	no,disturbance
R5457	T8882	T8883	nmod	Dhcr7,tissues
R5458	T8883	T8881	pobj	tissues,in
R5459	T8741	T8743	nsubjpass	disturbance,detected
R546	T1319	T1320	punct	-,5
R5460	T8884	T8882	punct	-,Dhcr7
R5461	T8885	T8882	punct	/,Dhcr7
R5462	T8742	T8741	amod	such,disturbance
R5463	T8886	T8882	punct	-,Dhcr7
R5464	T8887	T8883	amod	embryonic,tissues
R5465	T8888	T8871	prep	at,detect
R5466	T8743	T8717	conj	detected,resulted
R5467	T8889	T8888	pobj	E11.5,at
R5468	T8890	T8871	punct	.,detect
R5469	T8744	T8743	auxpass	was,detected
R547	T1320	T1318	prep	5,2
R5470	T8892	T8893	amod	Subtle,differences
R5471	T8893	T8894	nsubjpass	differences,excluded
R5472	T8895	T8893	prep	in,differences
R5473	T8896	T8897	compound	Shh,signal
R5474	T8745	T8743	punct	.,detected
R5475	T8897	T8898	compound	signal,transduction
R5476	T8898	T8895	pobj	transduction,in
R5477	T8899	T8894	aux	can,excluded
R5478	T8747	T8748	advmod	Indeed,seemed
R5479	T8900	T8894	neg	not,excluded
R548	T1321	T1318	punct	],2
R5480	T8901	T8894	auxpass	be,excluded
R5481	T8902	T8894	agent	by,excluded
R5482	T8903	T8904	det	the,study
R5483	T8749	T8748	punct	", ",seemed
R5484	T8904	T8902	pobj	study,by
R5485	T8905	T8904	amod	present,study
R5486	T8906	T8894	punct	.,excluded
R5487	T8750	T8751	det	the,patterns
R5488	T8908	T8909	advmod	However,favor
R5489	T8751	T8748	nsubj	patterns,seemed
R549	T1322	T1316	punct	", ",DHCR7
R5490	T8910	T8909	punct	", ",favor
R5491	T8911	T8909	nsubj	we,favor
R5492	T8912	T8913	det	a,model
R5493	T8752	T8751	prep	of,patterns
R5494	T8913	T8909	dobj	model,favor
R5495	T8914	T8915	advmod	whereby,is
R5496	T8915	T8913	relcl	is,model
R5497	T8753	T8754	compound	protein,expression
R5498	T8916	T8917	det	the,loss
R5499	T8917	T8915	nsubj	loss,is
R55	T300	T275	prep	within,die
R550	T1323	T1324	dep	which,encodes
R5500	T8918	T8917	prep	of,loss
R5501	T8754	T8752	pobj	expression,of
R5502	T8755	T8748	oprd	comparable,seemed
R5503	T8756	T8755	cc	and,comparable
R5504	T8757	T8755	conj	indistinguishable,comparable
R5505	T8919	T8920	amod	normal,biosynthesis
R5506	T8920	T8918	pobj	biosynthesis,of
R5507	T8758	T8757	prep	from,indistinguishable
R5508	T8921	T8920	compound	cholesterol,biosynthesis
R5509	T8922	T8915	acomp	important,is
R551	T1324	T1316	relcl	encodes,DHCR7
R5510	T8759	T8760	amod	wild,type
R5511	T8923	T8915	punct	", ",is
R5512	T8924	T8925	cc	not,for
R5513	T8760	T8762	compound	type,embryos
R5514	T8761	T8760	punct	-,type
R5515	T8925	T8915	prep	for,is
R5516	T8762	T8758	pobj	embryos,from
R5517	T8926	T8924	advmod	so,not
R5518	T8927	T8924	amod	much,not
R5519	T8763	T8748	prep	at,seemed
R552	T1325	T1324	prep	for,encodes
R5520	T8928	T8929	compound	Shh,autocleavage
R5521	T8929	T8925	pobj	autocleavage,for
R5522	T8930	T8925	punct	", ",for
R5523	T8931	T8925	cc	as,for
R5524	T8932	T8925	conj	for,for
R5525	T8933	T8934	det	the,loss
R5526	T8764	T8765	det	all,stages
R5527	T8934	T8932	pobj	loss,for
R5528	T8935	T8934	prep	of,loss
R5529	T8936	T8937	det	the,milieu
R553	T1326	T1327	nummod	7,dehydrocholesterol
R5530	T8937	T8935	pobj	milieu,of
R5531	T8765	T8763	pobj	stages,at
R5532	T8938	T8937	amod	correct,milieu
R5533	T8939	T8940	compound	plasma,membrane
R5534	T8940	T8937	compound	membrane,milieu
R5535	T8766	T8765	prep	of,stages
R5536	T8941	T8909	punct	.,favor
R5537	T8767	T8768	compound	lung,development
R5538	T8943	T8944	mark	Since,is
R5539	T8944	T8960	advcl	is,cause
R554	T1327	T1329	compound	dehydrocholesterol,reductase
R5540	T8945	T8946	det	the,loss
R5541	T8768	T8766	pobj	development,of
R5542	T8946	T8944	nsubj	loss,is
R5543	T8947	T8946	prep	of,loss
R5544	T8948	T8949	compound	cholesterol,content
R5545	T8769	T8765	acl	examined,stages
R5546	T8949	T8947	pobj	content,of
R5547	T8950	T8946	prep	in,loss
R5548	T8770	T8748	punct	.,seemed
R5549	T8951	T8952	det	the,membrane
R555	T1328	T1327	punct	-,dehydrocholesterol
R5550	T8952	T8950	pobj	membrane,in
R5551	T8953	T8952	compound	plasma,membrane
R5552	T8772	T8773	prep	In,showed
R5553	T8954	T8952	punct	(,membrane
R5554	T8955	T8952	cc	and,membrane
R5555	T8956	T8957	advmod	perhaps,membrane
R5556	T8957	T8952	conj	membrane,membrane
R5557	T8774	T8775	det	a,study
R5558	T8958	T8957	det	any,membrane
R5559	T8959	T8957	punct	),membrane
R556	T1329	T1325	pobj	reductase,for
R5560	T8961	T8944	acomp	likely,is
R5561	T8775	T8772	pobj	study,In
R5562	T8962	T8963	aux	to,alter
R5563	T8776	T8775	amod	recent,study
R5564	T8777	T8778	advmod	in,vitro
R5565	T8963	T8961	xcomp	alter,likely
R5566	T8964	T8965	det	the,function
R5567	T8778	T8775	amod	vitro,study
R5568	T8965	T8963	dobj	function,alter
R5569	T8966	T8965	prep	of,function
R557	T1330	T1329	compound	Δ7,reductase
R5570	T8967	T8966	pobj	receptors,of
R5571	T8968	T8969	advmod	more,sensitive
R5572	T8779	T8780	punct	[,43
R5573	T8969	T8967	amod	sensitive,receptors
R5574	T8970	T8969	prep	to,sensitive
R5575	T8971	T8970	pobj	this,to
R5576	T8972	T8960	punct	", ",cause
R5577	T8973	T8960	nsubj	loss,cause
R5578	T8974	T8973	prep	of,loss
R5579	T8780	T8775	parataxis	43,study
R558	T1331	T1329	punct	-,reductase
R5580	T8975	T8976	amod	normal,synthesis
R5581	T8976	T8974	pobj	synthesis,of
R5582	T8977	T8976	compound	cholesterol,synthesis
R5583	T8781	T8780	punct	],43
R5584	T8978	T8960	aux	may,cause
R5585	T8979	T8980	det	a,loss
R5586	T8980	T8960	dobj	loss,cause
R5587	T8782	T8773	punct	", ",showed
R5588	T8981	T8980	prep	of,loss
R5589	T8982	T8983	compound	signal,transduction
R559	T1332	T1324	cc	and,encodes
R5590	T8983	T8981	pobj	transduction,of
R5591	T8783	T8784	nmod	mouse,fibroblasts
R5592	T8984	T8983	prep	of,transduction
R5593	T8985	T8986	det	a,host
R5594	T8986	T8984	pobj	host,of
R5595	T8784	T8773	nsubj	fibroblasts,showed
R5596	T8987	T8986	prep	of,host
R5597	T8988	T8989	amod	such,pathways
R5598	T8989	T8987	pobj	pathways,of
R5599	T8785	T8784	amod	embryonic,fibroblasts
R56	T301	T302	nummod	24,hours
R560	T1333	T1324	conj	catalyzes,encodes
R5600	T8990	T8960	punct	.,cause
R5601	T8786	T8784	punct	(,fibroblasts
R5602	T8992	T8993	advmod	Thus,limited
R5603	T8994	T8993	punct	", ",limited
R5604	T8787	T8784	appos	MEFs,fibroblasts
R5605	T8995	T8996	det	this,effect
R5606	T8996	T8993	nsubjpass	effect,limited
R5607	T8997	T8993	aux	may,limited
R5608	T8788	T8784	punct	),fibroblasts
R5609	T8998	T8993	neg	not,limited
R561	T1334	T1335	det	a,step
R5610	T8999	T8993	auxpass	be,limited
R5611	T9000	T8993	prep	to,limited
R5612	T8789	T8784	prep	from,fibroblasts
R5613	T9001	T9002	det	the,pathway
R5614	T9002	T9000	pobj	pathway,to
R5615	T9003	T9002	compound	Shh,pathway
R5616	T8790	T8791	nmod	Dhcr7,embryos
R5617	T9004	T8993	cc	and,limited
R5618	T9005	T9006	amod	other,targets
R5619	T9006	T9007	nsubj	targets,be
R562	T1335	T1333	dobj	step,catalyzes
R5620	T9007	T8993	conj	be,limited
R5621	T8791	T8789	pobj	embryos,from
R5622	T9008	T9007	aux	may,be
R5623	T9009	T9010	advmod	more,important
R5624	T9010	T9007	acomp	important,be
R5625	T8792	T8790	punct	-,Dhcr7
R5626	T9011	T9007	punct	.,be
R5627	T9013	T9014	det	This,hypothesis
R5628	T9014	T9015	nsubj	hypothesis,is
R5629	T9016	T9015	acomp	amenable,is
R563	T1336	T1335	amod	final,step
R5630	T8793	T8790	punct	/,Dhcr7
R5631	T9017	T9016	prep	to,amenable
R5632	T9018	T9017	pobj	testing,to
R5633	T9019	T9015	prep	in,is
R5634	T8794	T8790	punct	-,Dhcr7
R5635	T9020	T9021	det	the,mice
R5636	T9021	T9019	pobj	mice,in
R5637	T9022	T9023	npadvmod	Dhcr7,null
R5638	T9023	T9021	amod	null,mice
R5639	T8795	T8791	punct	", ",embryos
R564	T1337	T1335	prep	of,step
R5640	T9024	T9015	punct	.,is
R5641	T8796	T8791	acl	grown,embryos
R5642	T8797	T8796	prep	in,grown
R5643	T8798	T8799	npadvmod	lipid,depleted
R5644	T8799	T8801	amod	depleted,culture
R5645	T8800	T8799	punct	-,depleted
R5646	T8801	T8797	pobj	culture,in
R5647	T8802	T8796	cc	and,grown
R5648	T8803	T8804	advmod	transiently,treated
R5649	T8804	T8796	conj	treated,grown
R565	T1338	T1339	compound	cholesterol,biosynthesis
R5650	T8805	T8804	prep	with,treated
R5651	T8806	T8805	pobj	cyclodextrin,with
R5652	T8807	T8773	punct	", ",showed
R5653	T8808	T8809	det	no,affect
R5654	T8809	T8773	dobj	affect,showed
R5655	T8810	T8809	prep	on,affect
R5656	T8811	T8812	compound	Shh,processing
R5657	T8812	T8810	pobj	processing,on
R566	T1339	T1337	pobj	biosynthesis,of
R5660	T9079	T9080	compound	Cholesterol,deficiency
R5661	T9080	T9081	nsubj	deficiency,resulted
R5662	T9082	T9080	acl	caused,deficiency
R5663	T9083	T9082	agent	by,caused
R5664	T9084	T9085	compound	Dhcr7,deficiency
R5665	T9085	T9083	pobj	deficiency,by
R5666	T9086	T9085	prep	in,deficiency
R5667	T9087	T9086	pobj	mice,in
R5668	T9088	T9081	prep	in,resulted
R5669	T9089	T9088	punct	", ",in
R567	T1340	T1298	punct	.,caused
R5670	T9090	T9091	prep	along,hypoplasia
R5671	T9091	T9088	pobj	hypoplasia,in
R5672	T9092	T9090	prep	with,along
R5673	T9093	T9092	pobj	IUGR,with
R5674	T9094	T9091	punct	", ",hypoplasia
R5675	T9095	T9091	det	a,hypoplasia
R5676	T9096	T9091	amod	distinct,hypoplasia
R5677	T9097	T9091	nmod	lung,hypoplasia
R5678	T9098	T9091	amod	saccular,hypoplasia
R5679	T9099	T9091	prep	with,hypoplasia
R568	T1342	T1343	nsubj	Recognition,strengthened
R5680	T9100	T9101	amod	impaired,differentiation
R5681	T9101	T9099	pobj	differentiation,with
R5682	T9102	T9101	prep	of,differentiation
R5683	T9103	T9104	nmod	type,AECs
R5684	T9104	T9102	pobj	AECs,of
R5685	T9105	T9103	nummod	I,type
R5686	T9106	T9103	cc	but,type
R5687	T9107	T9106	neg	not,but
R5688	T9108	T9103	conj	type,type
R5689	T9109	T9108	nummod	II,type
R569	T1344	T1342	prep	of,Recognition
R5690	T9110	T9091	cc	and,hypoplasia
R5691	T9111	T9112	amod	delayed,development
R5692	T9112	T9091	conj	development,hypoplasia
R5693	T9113	T9112	amod	associated,development
R5694	T9114	T9112	amod	vascular,development
R5695	T9115	T9081	punct	.,resulted
R5696	T9117	T9118	nsubj	Loss,perturb
R5697	T9119	T9117	prep	of,Loss
R5698	T9120	T9121	det	the,amounts
R5699	T9121	T9119	pobj	amounts,of
R57	T302	T300	pobj	hours,within
R570	T1345	T1346	amod	other,syndromes
R5700	T9122	T9121	amod	correct,amounts
R5701	T9123	T9121	prep	of,amounts
R5702	T9124	T9123	pobj	cholesterol,of
R5703	T9125	T9117	prep	in,Loss
R5704	T9126	T9127	det	the,membranes
R5705	T9127	T9125	pobj	membranes,in
R5706	T9128	T9118	aux	may,perturb
R5707	T9129	T9130	det	those,receptors
R5708	T9130	T9118	dobj	receptors,perturb
R5709	T9131	T9132	dep	that,depend
R571	T1346	T1344	pobj	syndromes,of
R5710	T9132	T9130	relcl	depend,receptors
R5711	T9133	T9132	prep	upon,depend
R5712	T9134	T9133	pobj	cholesterol,upon
R5713	T9135	T9132	prep	for,depend
R5714	T9136	T9137	amod	efficient,transduction
R5715	T9137	T9135	pobj	transduction,for
R5716	T9138	T9137	compound	signal,transduction
R5717	T9139	T9118	punct	.,perturb
R5718	T9141	T9142	amod	Further,investigation
R5719	T9142	T9143	nsubj	investigation,be
R572	T1347	T1346	compound	dysmorphology,syndromes
R5720	T9144	T9142	prep	of,investigation
R5721	T9145	T9146	det	which,pathways
R5722	T9146	T9151	dep	pathways,affected
R5723	T9147	T9146	amod	transcriptional,pathways
R5724	T9148	T9147	cc	and,transcriptional
R5725	T9149	T9150	compound	signal,transduction
R5726	T9150	T9147	conj	transduction,transcriptional
R5727	T9151	T9144	pcomp	affected,of
R5728	T9152	T9151	auxpass	are,affected
R5729	T9153	T9154	advmod	more,significantly
R573	T1348	T1346	acl	caused,syndromes
R5730	T9154	T9151	advmod	significantly,affected
R5731	T9155	T9151	agent	by,affected
R5732	T9156	T9157	det	the,loss
R5733	T9157	T9155	pobj	loss,by
R5734	T9158	T9157	prep	of,loss
R5735	T9159	T9158	pobj	Dhcr7,of
R5736	T9160	T9143	aux	will,be
R5737	T9161	T9143	acomp	critical,be
R5738	T9162	T9163	aux	to,elucidate
R5739	T9163	T9143	advcl	elucidate,be
R574	T1349	T1348	agent	by,caused
R5740	T9164	T9165	det	the,mechanism
R5741	T9165	T9163	dobj	mechanism,elucidate
R5742	T9166	T9165	prep	of,mechanism
R5743	T9167	T9168	compound	lung,hypoplasia
R5744	T9168	T9166	pobj	hypoplasia,of
R5745	T9169	T9168	prep	of,hypoplasia
R5746	T9170	T9171	det	this,model
R5747	T9171	T9169	pobj	model,of
R5748	T9172	T9165	punct	", ",mechanism
R5749	T9173	T9174	advmod	as,as
R575	T1350	T1349	pobj	defects,by
R5750	T9174	T9165	cc	as,mechanism
R5751	T9175	T9174	advmod	well,as
R5752	T9176	T9177	det	the,role
R5753	T9177	T9165	conj	role,mechanism
R5754	T9178	T9177	prep	of,role
R5755	T9179	T9178	pobj	cholesterol,of
R5756	T9180	T9177	prep	in,role
R5757	T9181	T9182	amod	embryonic,development
R5758	T9182	T9180	pobj	development,in
R5759	T9183	T9143	punct	.,be
R576	T1351	T1350	prep	in,defects
R5760	T9248	T9249	det	All,mice
R5761	T9249	T9250	nsubjpass	mice,housed
R5762	T9251	T9250	auxpass	were,housed
R5763	T9252	T9250	prep	in,housed
R5764	T9253	T9254	det	an,facility
R5765	T9254	T9252	pobj	facility,in
R5766	T9255	T9254	compound	animal,facility
R5767	T9256	T9250	prep	at,housed
R5768	T9257	T9258	nummod	22,°C
R5769	T9258	T9256	pobj	°C,at
R577	T1352	T1353	det	the,pathway
R5770	T9259	T9250	prep	with,housed
R5771	T9260	T9261	det	a,cycle
R5772	T9261	T9259	pobj	cycle,with
R5773	T9262	T9263	nummod	12,h
R5774	T9263	T9261	nmod	h,cycle
R5775	T9264	T9263	amod	light,h
R5776	T9265	T9263	punct	~,h
R5777	T9266	T9267	nummod	12,h
R5778	T9267	T9263	appos	h,h
R5779	T9268	T9267	amod	dark,h
R578	T1353	T1351	pobj	pathway,in
R5780	T9269	T9250	cc	and,housed
R5781	T9270	T9271	det	all,protocols
R5782	T9271	T9272	nsubjpass	protocols,approved
R5783	T9272	T9250	conj	approved,housed
R5784	T9273	T9272	auxpass	were,approved
R5785	T9274	T9272	agent	by,approved
R5786	T9275	T9276	det	the,Committee
R5787	T9276	T9274	pobj	Committee,by
R5788	T9277	T9278	compound	Institutional,Animal
R5789	T9278	T9279	compound	Animal,Welfare
R579	T1354	T1353	amod	post-squalene,pathway
R5790	T9279	T9276	compound	Welfare,Committee
R5791	T9280	T9272	punct	.,approved
R5792	T9282	T9283	npadvmod	Dhcr7,heterozygous
R5793	T9283	T9284	amod	heterozygous,animals
R5794	T9284	T9285	nsubjpass	animals,backcrossed
R5795	T9286	T9284	acl	used,animals
R5796	T9287	T9286	prep	in,used
R5797	T9288	T9289	det	this,study
R5798	T9289	T9287	pobj	study,in
R5799	T9290	T9285	aux	have,backcrossed
R58	T303	T302	prep	of,hours
R580	T1355	T1356	compound	cholesterol,biosynthesis
R5800	T9291	T9285	auxpass	been,backcrossed
R5801	T9292	T9285	prep	onto,backcrossed
R5802	T9293	T9294	det	a,background
R5803	T9294	T9292	pobj	background,onto
R5804	T9295	T9296	nmod	C57BL,6J
R5805	T9296	T9294	compound	6J,background
R5806	T9297	T9296	punct	/,6J
R5807	T9298	T9285	prep	for,backcrossed
R5808	T9299	T9300	nummod	11,generations
R5809	T9300	T9298	pobj	generations,for
R581	T1356	T1353	compound	biosynthesis,pathway
R5810	T9301	T9300	amod	successive,generations
R5811	T9302	T9285	punct	.,backcrossed
R5812	T9304	T9305	det	The,presence
R5813	T9305	T9306	nsubjpass	presence,considered
R5814	T9307	T9305	prep	of,presence
R5815	T9308	T9309	amod	vaginal,plugs
R5816	T9309	T9307	pobj	plugs,of
R5817	T9310	T9306	auxpass	was,considered
R5818	T9311	T9306	prep	as,considered
R5819	T9312	T9313	amod	embryonic,day
R582	T1357	T1350	punct	", ",defects
R5820	T9313	T9311	pobj	day,as
R5821	T9314	T9313	punct	(,day
R5822	T9315	T9313	appos	E,day
R5823	T9316	T9313	punct	),day
R5824	T9317	T9313	nummod	0.5,day
R5825	T9318	T9306	punct	.,considered
R5826	T9320	T9321	amod	Timed,females
R5827	T9321	T9323	nsubjpass	females,sacrificed
R5828	T9322	T9321	amod	pregnant,females
R5829	T9323	T9325	ccomp	sacrificed,dissected
R583	T1358	T1359	amod	such,as
R5830	T9324	T9323	auxpass	were,sacrificed
R5831	T9326	T9325	punct	", ",dissected
R5832	T9327	T9325	nsubj	embryos,dissected
R5833	T9328	T9325	prep	from,dissected
R5834	T9329	T9330	det	the,uteri
R5835	T9330	T9328	pobj	uteri,from
R5836	T9331	T9325	cc	and,dissected
R5837	T9332	T9325	conj	placed,dissected
R5838	T9333	T9332	prep	in,placed
R5839	T9334	T9335	compound	1X,PBS
R584	T1359	T1350	prep	as,defects
R5840	T9335	T9333	pobj	PBS,in
R5841	T9336	T9325	punct	.,dissected
R5842	T9338	T9339	nmod	Crown,lengths
R5843	T9339	T9342	nsubjpass	lengths,measured
R5844	T9340	T9341	punct	-,rump
R5845	T9341	T9338	prep	rump,Crown
R5846	T9343	T9339	prep	of,lengths
R5847	T9344	T9345	det	the,embryos
R5848	T9345	T9343	pobj	embryos,of
R5849	T9346	T9342	auxpass	were,measured
R585	T1360	T1359	pobj	desmosterolosis,as
R5850	T9347	T9342	prep	by,measured
R5851	T9348	T9347	pobj	use,by
R5852	T9349	T9348	prep	of,use
R5853	T9350	T9351	det	an,caliper
R5854	T9351	T9349	pobj	caliper,of
R5855	T9352	T9351	amod	electronic,caliper
R5856	T9353	T9351	amod	digital,caliper
R5857	T9354	T9342	cc	and,measured
R5858	T9355	T9356	nsubj	embryos,weighed
R5859	T9356	T9342	conj	weighed,measured
R586	T1361	T1360	punct	", ",desmosterolosis
R5860	T9357	T9356	punct	", ",weighed
R5861	T9358	T9356	prep	after,weighed
R5862	T9359	T9358	pcomp	blotting,after
R5863	T9360	T9359	prt	off,blotting
R5864	T9361	T9362	amod	excess,fluid
R5865	T9362	T9359	dobj	fluid,blotting
R5866	T9363	T9356	punct	.,weighed
R5867	T9365	T9366	det	Each,lung
R5868	T9366	T9367	nsubjpass	lung,removed
R5869	T9368	T9367	auxpass	was,removed
R587	T1362	T1360	appos	lathosterolosis,desmosterolosis
R5870	T9369	T9367	oprd	intact,removed
R5871	T9370	T9367	prep	from,removed
R5872	T9371	T9372	det	the,cavity
R5873	T9372	T9370	pobj	cavity,from
R5874	T9373	T9372	amod	thoracic,cavity
R5875	T9374	T9367	punct	", ",removed
R5876	T9375	T9367	conj	blotted,removed
R5877	T9376	T9375	oprd	free,blotted
R5878	T9377	T9376	prep	of,free
R5879	T9378	T9379	amod	excess,fluid
R588	T1363	T1360	punct	", ",desmosterolosis
R5880	T9379	T9377	pobj	fluid,of
R5881	T9380	T9375	punct	", ",blotted
R5882	T9381	T9375	cc	and,blotted
R5883	T9382	T9375	conj	weighed,blotted
R5884	T9383	T9367	punct	.,removed
R5885	T9385	T9386	nsubjpass	Mice,classified
R5886	T9387	T9386	auxpass	were,classified
R5887	T9388	T9386	prep	as,classified
R5888	T9389	T9388	pobj	P0,as
R5889	T9390	T9391	advmod	when,witnessed
R589	T1364	T1365	npadvmod	X,linked
R5890	T9391	T9386	advcl	witnessed,classified
R5891	T9392	T9391	nsubjpass	birth,witnessed
R5892	T9393	T9391	auxpass	was,witnessed
R5893	T9394	T9386	cc	and,classified
R5894	T9395	T9396	poss	their,activities
R5895	T9396	T9398	nsubj	activities,visualized
R5896	T9397	T9396	compound	breathing,activities
R5897	T9398	T9386	conj	visualized,classified
R5898	T9399	T9398	prep	under,visualized
R5899	T9400	T9401	det	a,microscope
R59	T304	T303	pobj	birth,of
R590	T1365	T1367	amod	linked,punctanta
R5900	T9401	T9399	pobj	microscope,under
R5901	T9402	T9401	amod	dissecting,microscope
R5902	T9403	T9398	punct	.,visualized
R5903	T9405	T9406	amod	Digital,images
R5904	T9406	T9407	nsubjpass	images,captured
R5905	T9408	T9406	prep	of,images
R5906	T9409	T9408	pobj	embryos,of
R5907	T9410	T9409	cc	or,embryos
R5908	T9411	T9409	conj	lungs,embryos
R5909	T9412	T9407	auxpass	were,captured
R591	T1366	T1365	punct	-,linked
R5910	T9413	T9407	advcl	using,captured
R5911	T9414	T9415	det	a,microscope
R5912	T9415	T9413	dobj	microscope,using
R5913	T9416	T9415	nmod	Zeiss,microscope
R5914	T9417	T9416	punct	-,Zeiss
R5915	T9418	T9416	nummod	1000,Zeiss
R5916	T9419	T9415	amod	dissecting,microscope
R5917	T9420	T9407	punct	.,captured
R5918	T9422	T9423	nsubjpass	Genotyping,performed
R5919	T9424	T9423	auxpass	was,performed
R592	T1367	T1360	appos	punctanta,desmosterolosis
R5920	T9425	T9423	prep	by,performed
R5921	T9426	T9425	pobj	PCR,by
R5922	T9427	T9423	punct	", ",performed
R5923	T9428	T9429	mark	as,described
R5924	T9429	T9423	advcl	described,performed
R5925	T9430	T9429	advmod	previously,described
R5926	T9431	T9432	punct	[,16
R5927	T9432	T9423	parataxis	16,performed
R5928	T9433	T9432	punct	],16
R5929	T9434	T9423	punct	.,performed
R593	T1368	T1367	compound	chondrodysplasia,punctanta
R5930	T9436	T9437	cc	And,performed
R5931	T9438	T9437	prep	in,performed
R5932	T9439	T9440	amod	selective,cases
R5933	T9440	T9438	pobj	cases,in
R5934	T9441	T9437	punct	", ",performed
R5935	T9442	T9437	nsubjpass	Southern,performed
R5936	T9443	T9442	amod	blotting,Southern
R5937	T9444	T9437	auxpass	was,performed
R5938	T9445	T9437	prep	for,performed
R5939	T9446	T9445	pobj	confirmation,for
R594	T1369	T1367	cc	and,punctanta
R5940	T9447	T9446	prep	of,confirmation
R5941	T9448	T9449	compound	Dhcr7,genotype
R5942	T9449	T9447	pobj	genotype,of
R5943	T9450	T9437	punct	.,performed
R5944	T9469	T9470	amod	Biochemical,measurements
R5945	T9472	T9473	compound	Tissue,sterol
R5946	T9473	T9474	compound	sterol,compositions
R5947	T9474	T9475	nsubjpass	compositions,analyzed
R5948	T9476	T9475	auxpass	were,analyzed
R5949	T9477	T9475	prep	by,analyzed
R595	T1370	T1371	compound	CHILD,syndrome
R5950	T9478	T9479	compound	gas,chromatography
R5951	T9479	T9477	pobj	chromatography,by
R5952	T9480	T9479	punct	-,chromatography
R5953	T9481	T9482	compound	mass,spectrometry
R5954	T9482	T9479	appos	spectrometry,chromatography
R5955	T9483	T9479	punct	(,chromatography
R5956	T9484	T9485	compound	GC,MS
R5957	T9485	T9479	appos	MS,chromatography
R5958	T9486	T9485	punct	-,MS
R5959	T9487	T9479	punct	),chromatography
R596	T1371	T1367	conj	syndrome,punctanta
R5960	T9488	T9489	mark	as,described
R5961	T9489	T9475	advcl	described,analyzed
R5962	T9490	T9489	advmod	previously,described
R5963	T9491	T9492	punct	[,44
R5964	T9492	T9489	parataxis	44,described
R5965	T9493	T9492	nummod	2,44
R5966	T9494	T9492	punct	",",44
R5967	T9495	T9492	punct	],44
R5968	T9496	T9475	punct	.,analyzed
R5969	T9498	T9499	compound	Serum,corticosterone
R597	T1372	T1373	punct	[,6
R5970	T9499	T9500	compound	corticosterone,levels
R5971	T9500	T9501	nsubjpass	levels,measured
R5972	T9502	T9501	auxpass	were,measured
R5973	T9503	T9501	prep	by,measured
R5974	T9504	T9503	pobj	radioimmunoassay,by
R5975	T9505	T9504	punct	(,radioimmunoassay
R5976	T9506	T9504	appos	RIA,radioimmunoassay
R5977	T9507	T9504	punct	),radioimmunoassay
R5978	T9508	T9504	prep	on,radioimmunoassay
R5979	T9509	T9510	det	the,blood
R598	T1373	T1348	parataxis	6,caused
R5980	T9510	T9508	pobj	blood,on
R5981	T9511	T9510	acl	obtained,blood
R5982	T9512	T9511	prep	from,obtained
R5983	T9513	T9514	amod	decapitated,neonates
R5984	T9514	T9512	pobj	neonates,from
R5985	T9515	T9511	agent	by,obtained
R5986	T9516	T9517	det	a,source
R5987	T9517	T9515	pobj	source,by
R5988	T9518	T9517	amod	commercial,source
R5989	T9519	T9520	punct	(,Lab
R599	T1374	T1375	punct	-,15
R5990	T9520	T9517	parataxis	Lab,source
R5991	T9521	T9520	nmod	DRTC,Lab
R5992	T9522	T9520	nmod	hormone,Lab
R5993	T9523	T9520	nmod	Assay,Lab
R5994	T9524	T9520	amod	Core,Lab
R5995	T9525	T9520	punct	", ",Lab
R5996	T9526	T9527	compound	Vanderbilt,University
R5997	T9527	T9520	npadvmod	University,Lab
R5998	T9528	T9520	punct	", ",Lab
R5999	T9529	T9520	npadvmod	TN,Lab
R6	T244	T243	compound	lung,hypoplasia
R60	T305	T275	punct	.,die
R600	T1375	T1373	prep	15,6
R6000	T9530	T9520	punct	),Lab
R6001	T9531	T9501	punct	.,measured
R6002	T9619	T9618	cc	and,Histology
R6003	T9620	T9618	conj	Immunohistochemistry,Histology
R6004	T9622	T9623	amod	Thoracic,parts
R6005	T9623	T9624	nsubjpass	parts,fixed
R6006	T9625	T9623	prep	from,parts
R6007	T9626	T9625	pobj	embryos,from
R6008	T9627	T9626	cc	or,embryos
R6009	T9628	T9629	compound	P0,mice
R601	T1376	T1375	punct	],15
R6010	T9629	T9626	conj	mice,embryos
R6011	T9630	T9624	auxpass	were,fixed
R6012	T9631	T9624	prep	in,fixed
R6013	T9632	T9633	preconj	either,fixative
R6014	T9633	T9631	pobj	fixative,in
R6015	T9634	T9633	poss	Amsterdam,fixative
R6016	T9635	T9634	case	's,Amsterdam
R6017	T9636	T9637	punct	(,35
R6018	T9637	T9633	parataxis	35,fixative
R6019	T9638	T9637	dep	methanol,35
R602	T1377	T1343	punct	", ",strengthened
R6020	T9639	T9640	punct	:,acetone
R6021	T9640	T9638	prep	acetone,methanol
R6022	T9641	T9642	punct	:,acid
R6023	T9642	T9640	prep	acid,acetone
R6024	T9643	T9642	amod	acetic,acid
R6025	T9644	T9645	punct	:,water
R6026	T9645	T9640	prep	water,acetone
R6027	T9646	T9637	punct	", ",35
R6028	T9647	T9648	punct	:,35
R6029	T9648	T9637	prep	35,35
R603	T1378	T1343	aux	has,strengthened
R6030	T9649	T9650	punct	:,5
R6031	T9650	T9648	prep	5,35
R6032	T9651	T9652	punct	:,25
R6033	T9652	T9648	prep	25,35
R6034	T9653	T9637	npadvmod	v,35
R6035	T9654	T9655	punct	/,v
R6036	T9655	T9653	prep	v,v
R6037	T9656	T9637	punct	),35
R6038	T9657	T9633	cc	or,fixative
R6039	T9658	T9659	nummod	4,%
R604	T1379	T1380	det	this,concept
R6040	T9659	T9660	compound	%,paraformaldehyde
R6041	T9660	T9633	conj	paraformaldehyde,fixative
R6042	T9661	T9624	prep	in,fixed
R6043	T9662	T9661	pobj	PBS,in
R6044	T9663	T9624	punct	.,fixed
R6045	T9665	T9666	nsubjpass	Tissues,fixed
R6046	T9667	T9666	auxpass	were,fixed
R6047	T9668	T9666	advmod	overnight,fixed
R6048	T9669	T9666	prep	at,fixed
R6049	T9670	T9671	compound	room,temperature
R605	T1380	T1343	dobj	concept,strengthened
R6050	T9671	T9669	pobj	temperature,at
R6051	T9672	T9671	punct	(,temperature
R6052	T9673	T9671	appos	RT,temperature
R6053	T9674	T9671	punct	),temperature
R6054	T9675	T9666	punct	", ",fixed
R6055	T9676	T9666	conj	rinsed,fixed
R6056	T9677	T9676	prep	in,rinsed
R6057	T9678	T9677	pobj	PBS,in
R6058	T9679	T9676	punct	", ",rinsed
R6059	T9680	T9676	conj	dehydrated,rinsed
R606	T1381	T1343	punct	.,strengthened
R6060	T9681	T9680	prep	in,dehydrated
R6061	T9682	T9683	det	a,series
R6062	T9683	T9681	pobj	series,in
R6063	T9684	T9683	amod	graded,series
R6064	T9685	T9683	compound	ethanol,series
R6065	T9686	T9680	punct	", ",dehydrated
R6066	T9687	T9680	conj	cleared,dehydrated
R6067	T9688	T9687	prep	in,cleared
R6068	T9689	T9688	pobj	toluene,in
R6069	T9690	T9687	cc	and,cleared
R607	T1383	T1384	amod	Genetic,disruption
R6070	T9691	T9687	conj	embedded,cleared
R6071	T9692	T9691	prep	in,embedded
R6072	T9693	T9692	pobj	paraffin,in
R6073	T9694	T9666	punct	.,fixed
R6074	T9696	T9697	nummod	Five,m
R6075	T9697	T9699	compound	m,sections
R6076	T9698	T9697	compound	μ,m
R6077	T9699	T9700	nsubjpass	sections,mounted
R6078	T9701	T9700	auxpass	were,mounted
R6079	T9702	T9700	prep	on,mounted
R608	T1384	T1385	nsubj	disruption,results
R6080	T9703	T9704	compound	Superfrost,Plus
R6081	T9704	T9706	compound	Plus,slides
R6082	T9705	T9704	punct	-,Plus
R6083	T9706	T9702	pobj	slides,on
R6084	T9707	T9708	punct	(,VWR
R6085	T9708	T9706	parataxis	VWR,slides
R6086	T9709	T9708	punct	", ",VWR
R6087	T9710	T9711	compound	West,Chester
R6088	T9711	T9708	npadvmod	Chester,VWR
R6089	T9712	T9708	punct	", ",VWR
R609	T1386	T1384	prep	of,disruption
R6090	T9713	T9708	npadvmod	PA,VWR
R6091	T9714	T9708	punct	),VWR
R6092	T9715	T9700	prep	for,mounted
R6093	T9716	T9717	amod	histological,analyses
R6094	T9717	T9715	pobj	analyses,for
R6095	T9718	T9716	cc	or,histological
R6096	T9719	T9716	conj	immunohistochemistry,histological
R6097	T9720	T9719	punct	(,immunohistochemistry
R6098	T9721	T9719	appos	IHC,immunohistochemistry
R6099	T9722	T9719	punct	),immunohistochemistry
R61	T307	T308	mark	Although,have
R610	T1387	T1388	det	the,Dhcr7
R6100	T9723	T9700	punct	.,mounted
R6101	T9725	T9726	nsubjpass	IHC,performed
R6102	T9727	T9726	auxpass	was,performed
R6103	T9728	T9726	advcl	using,performed
R6104	T9729	T9730	det	an,method
R6105	T9730	T9728	dobj	method,using
R6106	T9731	T9730	amod	indirect,method
R6107	T9732	T9726	punct	.,performed
R6108	T9734	T9735	prep	After,heated
R6109	T9736	T9734	pobj	deparaffination,After
R611	T1388	T1386	pobj	Dhcr7,of
R6110	T9737	T9736	prep	in,deparaffination
R6111	T9738	T9737	pobj	xylene,in
R6112	T9739	T9736	cc	and,deparaffination
R6113	T9740	T9736	conj	rehydration,deparaffination
R6114	T9741	T9740	prep	through,rehydration
R6115	T9742	T9743	det	a,series
R6116	T9743	T9741	pobj	series,through
R6117	T9744	T9743	amod	graded,series
R6118	T9745	T9743	prep	of,series
R6119	T9746	T9745	pobj	ethanol,of
R612	T1389	T1385	prep	in,results
R6120	T9747	T9735	punct	", ",heated
R6121	T9748	T9735	nsubjpass	sections,heated
R6122	T9749	T9735	auxpass	were,heated
R6123	T9750	T9735	prep	at,heated
R6124	T9751	T9752	nummod	95,°C
R6125	T9752	T9750	pobj	°C,at
R6126	T9753	T9735	prep	in,heated
R6127	T9754	T9755	nummod	10,mM
R6128	T9755	T9756	compound	mM,citrate
R6129	T9756	T9753	pobj	citrate,in
R613	T1390	T1391	amod	neonatal,lethality
R6130	T9757	T9756	compound	sodium,citrate
R6131	T9758	T9756	punct	(,citrate
R6132	T9759	T9756	npadvmod	pH,citrate
R6133	T9760	T9759	nummod	6.0,pH
R6134	T9761	T9756	punct	),citrate
R6135	T9762	T9735	prep	for,heated
R6136	T9763	T9764	det	an,min
R6137	T9764	T9762	pobj	min,for
R6138	T9765	T9764	amod	initial,min
R6139	T9766	T9764	nummod	15,min
R614	T1391	T1389	pobj	lethality,in
R6140	T9767	T9762	cc	and,for
R6141	T9768	T9762	conj	for,for
R6142	T9769	T9770	nummod	three,periods
R6143	T9770	T9768	pobj	periods,for
R6144	T9771	T9770	amod	successive,periods
R6145	T9772	T9773	nummod	5,min
R6146	T9773	T9770	compound	min,periods
R6147	T9774	T9773	punct	-,min
R6148	T9775	T9776	punct	[,25
R6149	T9776	T9735	parataxis	25,heated
R615	T1392	T1393	punct	[,16
R6150	T9777	T9776	punct	],25
R6151	T9778	T9735	punct	.,heated
R6152	T9780	T9781	prep	After,treated
R6153	T9782	T9780	pobj	cooling,After
R6154	T9783	T9781	punct	", ",treated
R6155	T9784	T9785	det	the,sections
R6156	T9785	T9781	nsubjpass	sections,treated
R6157	T9786	T9781	auxpass	were,treated
R6158	T9787	T9781	prep	with,treated
R6159	T9788	T9789	compound	hydrogen,peroxide
R616	T1393	T1385	parataxis	16,results
R6160	T9789	T9787	pobj	peroxide,with
R6161	T9790	T9791	punct	(,v
R6162	T9791	T9789	parataxis	v,peroxide
R6163	T9792	T9793	nummod	3,%
R6164	T9793	T9791	compound	%,v
R6165	T9794	T9795	punct	/,v
R6166	T9795	T9791	prep	v,v
R6167	T9796	T9791	prep	in,v
R6168	T9797	T9796	pobj	PBS,in
R6169	T9798	T9791	punct	", ",v
R617	T1394	T1393	punct	],16
R6170	T9799	T9791	npadvmod	pH7.4,v
R6171	T9800	T9791	punct	),v
R6172	T9801	T9781	prep	for,treated
R6173	T9802	T9803	nummod	10,min
R6174	T9803	T9801	pobj	min,for
R6175	T9804	T9781	cc	and,treated
R6176	T9805	T9781	conj	rinsed,treated
R6177	T9806	T9805	prep	in,rinsed
R6178	T9807	T9808	det	the,PBS
R6179	T9808	T9806	pobj	PBS,in
R618	T1395	T1385	punct	.,results
R6180	T9809	T9781	punct	.,treated
R6181	T9811	T9812	nsubjpass	Sections,blocked
R6182	T9813	T9812	auxpass	were,blocked
R6183	T9814	T9812	prep	with,blocked
R6184	T9815	T9816	nummod	5,%
R6185	T9816	T9817	nmod	%,serum
R6186	T9817	T9814	pobj	serum,with
R6187	T9818	T9817	amod	normal,serum
R6188	T9819	T9812	prep	for,blocked
R6189	T9820	T9821	nummod	2,h
R619	T1397	T1398	amod	Neonatal,mice
R6190	T9821	T9819	pobj	h,for
R6191	T9822	T9812	punct	", ",blocked
R6192	T9823	T9812	dep	washed,blocked
R6193	T9824	T9823	prep	in,washed
R6194	T9825	T9824	pobj	PBS,in
R6195	T9826	T9823	cc	and,washed
R6196	T9827	T9823	conj	incubated,washed
R6197	T9828	T9827	prep	with,incubated
R6198	T9829	T9828	pobj	antibodies,with
R6199	T9830	T9829	prep	against,antibodies
R62	T308	T310	advcl	have,is
R620	T1398	T1399	nsubj	mice,are
R6200	T9831	T9832	compound	Shh,N19
R6201	T9832	T9830	pobj	N19,against
R6202	T9833	T9832	punct	-,N19
R6203	T9834	T9835	punct	(,1
R6204	T9835	T9832	parataxis	1,N19
R6205	T9836	T9835	punct	:,1
R6206	T9837	T9835	appos	100,1
R6207	T9838	T9835	punct	),1
R6208	T9839	T9832	punct	", ",N19
R6209	T9840	T9832	conj	Patched,N19
R621	T1400	T1398	amod	homozygous,mice
R6210	T9841	T9842	punct	(,1
R6211	T9842	T9840	parataxis	1,Patched
R6212	T9843	T9844	punct	:,100
R6213	T9844	T9842	prep	100,1
R6214	T9845	T9842	punct	),1
R6215	T9846	T9840	punct	", ",Patched
R6216	T9847	T9848	compound	Smo,N19
R6217	T9848	T9840	conj	N19,Patched
R6218	T9849	T9848	punct	-,N19
R6219	T9850	T9851	punct	(,1
R622	T1401	T1400	prep	for,homozygous
R6220	T9851	T9848	parataxis	1,N19
R6221	T9852	T9853	punct	:,100
R6222	T9853	T9851	prep	100,1
R6223	T9854	T9851	punct	),1
R6224	T9855	T9848	punct	", ",N19
R6225	T9856	T9848	conj	Gli1,N19
R6226	T9857	T9858	punct	(,1
R6227	T9858	T9856	parataxis	1,Gli1
R6228	T9859	T9860	punct	:,100
R6229	T9860	T9858	prep	100,1
R623	T1402	T1403	det	the,disruption
R6230	T9861	T9858	punct	),1
R6231	T9862	T9856	punct	", ",Gli1
R6232	T9863	T9864	compound	Gli3,N19
R6233	T9864	T9856	conj	N19,Gli1
R6234	T9865	T9864	punct	-,N19
R6235	T9866	T9867	punct	(,1
R6236	T9867	T9864	parataxis	1,N19
R6237	T9868	T9869	punct	:,100
R6238	T9869	T9867	prep	100,1
R6239	T9870	T9867	punct	),1
R624	T1403	T1401	pobj	disruption,for
R6240	T9871	T9864	punct	", ",N19
R6241	T9872	T9873	compound	SP,C
R6242	T9873	T9875	compound	C,C19
R6243	T9874	T9873	punct	-,C
R6244	T9875	T9864	conj	C19,N19
R6245	T9876	T9877	punct	(,1
R6246	T9877	T9875	parataxis	1,C19
R6247	T9878	T9879	punct	:,200
R6248	T9879	T9877	prep	200,1
R6249	T9880	T9877	punct	),1
R625	T1404	T1405	compound	Dhcr7,gene
R6250	T9881	T9875	punct	", ",C19
R6251	T9882	T9883	nmod	caveolin,N20
R6252	T9883	T9875	conj	N20,C19
R6253	T9884	T9883	nummod	1,N20
R6254	T9885	T9883	compound	α,N20
R6255	T9886	T9883	punct	-,N20
R6256	T9887	T9888	punct	(,1
R6257	T9888	T9883	parataxis	1,N20
R6258	T9889	T9890	punct	:,200
R6259	T9890	T9888	prep	200,1
R626	T1405	T1403	compound	gene,disruption
R6260	T9891	T9888	punct	),1
R6261	T9892	T9883	punct	", ",N20
R6262	T9893	T9883	conj	aquaporin,N20
R6263	T9894	T9893	nummod	5,aquaporin
R6264	T9895	T9893	punct	(,aquaporin
R6265	T9896	T9893	appos	AQP5,aquaporin
R6266	T9897	T9898	punct	", ",1
R6267	T9898	T9893	parataxis	1,aquaporin
R6268	T9899	T9900	punct	:,200
R6269	T9900	T9898	prep	200,1
R627	T1406	T1399	acomp	deficient,are
R6270	T9901	T9898	punct	),1
R6271	T9902	T9893	punct	", ",aquaporin
R6272	T9903	T9893	conj	megalin,aquaporin
R6273	T9904	T9905	punct	(,1
R6274	T9905	T9903	parataxis	1,megalin
R6275	T9906	T9907	punct	:,200
R6276	T9907	T9905	prep	200,1
R6277	T9908	T9905	punct	),1
R6278	T9909	T9903	punct	", ",megalin
R6279	T9910	T9903	conj	PECAM,megalin
R628	T1407	T1406	prep	in,deficient
R6280	T9911	T9910	punct	-,PECAM
R6281	T9912	T9910	nummod	1,PECAM
R6282	T9913	T9914	punct	(,1
R6283	T9914	T9910	parataxis	1,PECAM
R6284	T9915	T9916	punct	:,200
R6285	T9916	T9914	prep	200,1
R6286	T9917	T9914	punct	),1
R6287	T9918	T9919	punct	(,Biotechnology
R6288	T9919	T9910	parataxis	Biotechnology,PECAM
R6289	T9920	T9921	compound	Santa,Cruz
R629	T1408	T1407	pobj	cholesterol,in
R6290	T9921	T9919	compound	Cruz,Biotechnology
R6291	T9922	T9919	punct	", ",Biotechnology
R6292	T9923	T9924	compound	Santa,Cruz
R6293	T9924	T9919	npadvmod	Cruz,Biotechnology
R6294	T9925	T9919	punct	", ",Biotechnology
R6295	T9926	T9919	npadvmod	CA,Biotechnology
R6296	T9927	T9919	punct	),Biotechnology
R6297	T9928	T9910	cc	or,PECAM
R6298	T9929	T9910	conj	T1α,PECAM
R6299	T9930	T9931	punct	(,Bank
R63	T309	T308	nsubj	they,have
R630	T1409	T1399	cc	and,are
R6300	T9931	T9929	parataxis	Bank,T1α
R6301	T9932	T9931	dep	mAB,Bank
R6302	T9933	T9932	nummod	8.1.1,mAB
R6303	T9934	T9931	punct	", ",Bank
R6304	T9935	T9936	nummod	1,dilution
R6305	T9936	T9931	dep	dilution,Bank
R6306	T9937	T9938	punct	:,1000
R6307	T9938	T9935	prep	1000,1
R6308	T9939	T9931	punct	", ",Bank
R6309	T9940	T9941	compound	Developmental,Studies
R631	T1410	T1399	conj	have,are
R6310	T9941	T9931	compound	Studies,Bank
R6311	T9942	T9931	compound	Hybridoma,Bank
R6312	T9943	T9931	punct	", ",Bank
R6313	T9944	T9945	compound	Iowa,City
R6314	T9945	T9931	npadvmod	City,Bank
R6315	T9946	T9931	punct	", ",Bank
R6316	T9947	T9931	npadvmod	IA,Bank
R6317	T9948	T9931	punct	),Bank
R6318	T9949	T9827	advmod	overnight,incubated
R6319	T9950	T9827	prep	at,incubated
R632	T1411	T1412	amod	increased,accumulation
R6320	T9951	T9952	nummod	4,C
R6321	T9952	T9950	pobj	C,at
R6322	T9953	T9952	compound	°,C
R6323	T9954	T9827	prep	in,incubated
R6324	T9955	T9956	det	a,chamber
R6325	T9956	T9954	pobj	chamber,in
R6326	T9957	T9956	amod	humidified,chamber
R6327	T9958	T9812	punct	.,blocked
R6328	T9960	T9961	det	The,sections
R6329	T9961	T9962	nsubjpass	sections,washed
R633	T1412	T1410	dobj	accumulation,have
R6330	T9963	T9962	auxpass	were,washed
R6331	T9964	T9962	prep	in,washed
R6332	T9965	T9964	pobj	PBS,in
R6333	T9966	T9962	punct	", ",washed
R6334	T9967	T9962	conj	incubated,washed
R6335	T9968	T9967	prep	with,incubated
R6336	T9969	T9970	amod	biotinylated,antibodies
R6337	T9970	T9968	pobj	antibodies,with
R6338	T9971	T9970	amod	secondary,antibodies
R6339	T9972	T9973	punct	(,1
R634	T1413	T1412	prep	of,accumulation
R6340	T9973	T9970	parataxis	1,antibodies
R6341	T9974	T9975	punct	:,1000
R6342	T9975	T9973	prep	1000,1
R6343	T9976	T9977	punct	~,1
R6344	T9977	T9973	prep	1,1
R6345	T9978	T9979	punct	:,5000
R6346	T9979	T9977	prep	5000,1
R6347	T9980	T9973	punct	", ",1
R6348	T9981	T9982	compound	Santa,Cruz
R6349	T9982	T9983	compound	Cruz,Biotechnology
R635	T1414	T1415	det	the,precursor
R6350	T9983	T9973	npadvmod	Biotechnology,1
R6351	T9984	T9973	punct	),1
R6352	T9985	T9967	prep	in,incubated
R6353	T9986	T9985	pobj	PBS,in
R6354	T9987	T9967	prep	for,incubated
R6355	T9988	T9989	nummod	2,h
R6356	T9989	T9987	pobj	h,for
R6357	T9990	T9967	prep	at,incubated
R6358	T9991	T9990	pobj	RT,at
R6359	T9992	T9967	punct	", ",incubated
R636	T1415	T1413	pobj	precursor,of
R6360	T9993	T9967	conj	incubated,incubated
R6361	T9994	T9993	prep	with,incubated
R6362	T9995	T9996	compound	HRP,streptavidin
R6363	T9996	T9998	compound	streptavidin,complex
R6364	T9997	T9996	punct	-,streptavidin
R6365	T9998	T9994	pobj	complex,with
R6366	T9999	T9993	prep	for,incubated
R6367	T10000	T10001	nummod	30,min
R6368	T10001	T9999	pobj	min,for
R6369	T10002	T9993	punct	", ",incubated
R637	T1416	T1415	compound	cholesterol,precursor
R6370	T10003	T9993	conj	rinsed,incubated
R6371	T10004	T10003	prep	in,rinsed
R6372	T10005	T10004	pobj	PBS,in
R6373	T10006	T10003	cc	and,rinsed
R6374	T10007	T10003	conj	visualized,rinsed
R6375	T10008	T10007	advcl	using,visualized
R6376	T10009	T10010	det	a,Kit
R6377	T10010	T10008	dobj	Kit,using
R6378	T10011	T10012	npadvmod	Metal,Enhanced
R6379	T10012	T10013	amod	Enhanced,Diaminobenzidine
R638	T1417	T1418	nummod	7,dehydrocholesterol
R6380	T10013	T10010	nmod	Diaminobenzidine,Kit
R6381	T10014	T10013	punct	(,Diaminobenzidine
R6382	T10015	T10013	appos	DAB,Diaminobenzidine
R6383	T10016	T10013	punct	),Diaminobenzidine
R6384	T10017	T10010	compound	Substrate,Kit
R6385	T10018	T10019	punct	(,Pierce
R6386	T10019	T10010	parataxis	Pierce,Kit
R6387	T10020	T10019	punct	", ",Pierce
R6388	T10021	T10019	npadvmod	Rockford,Pierce
R6389	T10022	T10019	punct	", ",Pierce
R639	T1418	T1415	appos	dehydrocholesterol,precursor
R6390	T10023	T10019	npadvmod	IL,Pierce
R6391	T10024	T10019	punct	),Pierce
R6392	T10025	T9962	punct	.,washed
R6393	T10027	T10028	amod	Digital,images
R6394	T10028	T10029	nsubjpass	images,collected
R6395	T10030	T10029	auxpass	were,collected
R6396	T10031	T10029	advcl	using,collected
R6397	T10032	T10033	det	a,Camera
R6398	T10033	T10031	dobj	Camera,using
R6399	T10034	T10033	compound	Polaroid,Camera
R64	T311	T312	det	a,number
R640	T1419	T1418	punct	-,dehydrocholesterol
R6400	T10035	T10033	compound	Digital,Camera
R6401	T10036	T10033	compound	Microscope,Camera
R6402	T10037	T10033	acl	mounted,Camera
R6403	T10038	T10037	prep	on,mounted
R6404	T10039	T10040	det	an,microscope
R6405	T10040	T10038	pobj	microscope,on
R6406	T10041	T10042	compound	Olympus,BX40
R6407	T10042	T10040	compound	BX40,microscope
R6408	T10043	T10029	punct	.,collected
R6409	T10060	T10061	compound	Lung,morphometry
R641	T1420	T1418	punct	(,dehydrocholesterol
R6410	T10063	T10064	amod	Terminal,space
R6411	T10064	T10066	compound	space,areas
R6412	T10065	T10064	amod	saccular,space
R6413	T10066	T10067	nsubjpass	areas,measured
R6414	T10068	T10067	auxpass	were,measured
R6415	T10069	T10067	advcl	using,measured
R6416	T10070	T10071	compound	NIH,software
R6417	T10071	T10069	dobj	software,using
R6418	T10072	T10071	compound	Image,software
R6419	T10073	T10074	punct	(,NIH
R642	T1421	T1418	appos	7DHC,dehydrocholesterol
R6420	T10074	T10071	parataxis	NIH,software
R6421	T10075	T10074	punct	", ",NIH
R6422	T10076	T10074	npadvmod	Bethesda,NIH
R6423	T10077	T10074	punct	", ",NIH
R6424	T10078	T10074	npadvmod	MD,NIH
R6425	T10079	T10074	punct	),NIH
R6426	T10080	T10081	mark	as,described
R6427	T10081	T10069	advcl	described,using
R6428	T10082	T10081	advmod	previously,described
R6429	T10083	T10084	punct	[,45
R643	T1422	T1399	punct	),are
R6430	T10084	T10081	parataxis	45,described
R6431	T10085	T10084	punct	],45
R6432	T10086	T10067	punct	.,measured
R6433	T10088	T10089	amod	Multiple,measurements
R6434	T10089	T10090	nsubjpass	measurements,performed
R6435	T10091	T10090	auxpass	were,performed
R6436	T10092	T10090	prep	on,performed
R6437	T10093	T10094	advmod	randomly,selected
R6438	T10094	T10095	amod	selected,fields
R6439	T10095	T10092	pobj	fields,on
R644	T1423	T1399	punct	.,are
R6440	T10096	T10097	nummod	0.04,mm2
R6441	T10097	T10095	compound	mm2,fields
R6442	T10098	T10097	punct	-,mm2
R6443	T10099	T10095	acl	located,fields
R6444	T10100	T10099	prep	in,located
R6445	T10101	T10102	det	the,part
R6446	T10102	T10100	pobj	part,in
R6447	T10103	T10102	amod	distal,part
R6448	T10104	T10102	prep	of,part
R6449	T10105	T10106	det	the,sections
R645	T1425	T1426	mark	Although,noted
R6450	T10106	T10104	pobj	sections,of
R6451	T10107	T10106	compound	lung,sections
R6452	T10108	T10090	punct	.,performed
R6453	T10110	T10111	det	The,proportion
R6454	T10111	T10112	nsubjpass	proportion,calculated
R6455	T10113	T10111	prep	of,proportion
R6456	T10114	T10113	pobj	lung,of
R6457	T10115	T10111	acl	comprising,proportion
R6458	T10116	T10117	amod	terminal,spaces
R6459	T10117	T10115	dobj	spaces,comprising
R646	T1426	T1431	advcl	noted,exhibited
R6460	T10118	T10117	amod	saccular,spaces
R6461	T10119	T10112	auxpass	was,calculated
R6462	T10120	T10112	prep	as,calculated
R6463	T10121	T10122	det	a,percentage
R6464	T10122	T10120	pobj	percentage,as
R6465	T10123	T10122	prep	of,percentage
R6466	T10124	T10125	det	the,area
R6467	T10125	T10123	pobj	area,of
R6468	T10126	T10125	amod	total,area
R6469	T10127	T10125	amod	examined,area
R647	T1427	T1428	det	no,lethality
R6470	T10128	T10125	prep	of,area
R6471	T10129	T10130	det	the,section
R6472	T10130	T10128	pobj	section,of
R6473	T10131	T10130	compound	lung,section
R6474	T10132	T10112	punct	.,calculated
R6475	T10134	T10135	nummod	Three,animals
R6476	T10135	T10136	nsubjpass	animals,examined
R6477	T10137	T10135	prep	per,animals
R6478	T10138	T10137	pobj	genotype,per
R6479	T10139	T10136	auxpass	were,examined
R648	T1428	T1426	nsubjpass	lethality,noted
R6480	T10140	T10136	punct	.,examined
R6487	T10258	T10257	prep	of,Assessment
R6488	T10259	T10260	nmod	cell,death
R6489	T10260	T10258	pobj	death,of
R649	T1429	T1428	amod	embryonic,lethality
R6490	T10261	T10260	nmod	proliferation,death
R6491	T10262	T10260	punct	/,death
R6492	T10263	T10260	nmod	Division,death
R6493	T10264	T10260	punct	/,death
R6494	T10266	T10267	nsubjpass	Changes,assessed
R6495	T10268	T10266	prep	in,Changes
R6496	T10269	T10270	compound	cell,proliferation
R6497	T10270	T10268	pobj	proliferation,in
R6498	T10271	T10270	cc	and,proliferation
R6499	T10272	T10270	conj	division,proliferation
R65	T312	T308	dobj	number,have
R650	T1430	T1426	auxpass	was,noted
R6500	T10273	T10267	auxpass	were,assessed
R6501	T10274	T10267	prep	by,assessed
R6502	T10275	T10276	amod	proliferating,antigen
R6503	T10276	T10279	nmod	antigen,IHC
R6504	T10277	T10276	nmod	cell,antigen
R6505	T10278	T10276	amod	nuclear,antigen
R6506	T10279	T10274	pobj	IHC,by
R6507	T10280	T10276	punct	(,antigen
R6508	T10281	T10276	appos	PCNA,antigen
R6509	T10282	T10279	punct	),IHC
R651	T1432	T1431	punct	", ",exhibited
R6510	T10283	T10279	punct	", ",IHC
R6511	T10284	T10285	nmod	bromodeoxyuridine,incorporation
R6512	T10285	T10279	conj	incorporation,IHC
R6513	T10286	T10284	punct	(,bromodeoxyuridine
R6514	T10287	T10284	appos	BrdU,bromodeoxyuridine
R6515	T10288	T10285	punct	),incorporation
R6516	T10289	T10285	cc	and,incorporation
R6517	T10290	T10291	amod	phosphorylated,H3
R6518	T10291	T10293	nmod	H3,IHC
R6519	T10292	T10291	nmod	histone,H3
R652	T1433	T1434	det	these,pups
R6520	T10293	T10285	conj	IHC,incorporation
R6521	T10294	T10291	punct	(,H3
R6522	T10295	T10291	appos	pHH3,H3
R6523	T10296	T10293	punct	),IHC
R6524	T10297	T10267	punct	.,assessed
R6525	T10299	T10300	det	The,PCNA
R6526	T10300	T10301	nmod	PCNA,antibodies
R6527	T10301	T10316	nsubjpass	antibodies,obtained
R6528	T10302	T10303	punct	(,1
R6529	T10303	T10300	parataxis	1,PCNA
R653	T1434	T1431	nsubj	pups,exhibited
R6530	T10304	T10305	punct	:,2000
R6531	T10305	T10303	prep	2000,1
R6532	T10306	T10303	punct	),1
R6533	T10307	T10300	cc	and,PCNA
R6534	T10308	T10309	compound	pHH3,Ser28
R6535	T10309	T10300	conj	Ser28,PCNA
R6536	T10310	T10309	punct	-,Ser28
R6537	T10311	T10312	punct	(,1
R6538	T10312	T10309	parataxis	1,Ser28
R6539	T10313	T10314	punct	:,200
R654	T1435	T1434	compound	knockout,pups
R6540	T10314	T10312	prep	200,1
R6541	T10315	T10312	punct	),1
R6542	T10317	T10316	auxpass	were,obtained
R6543	T10318	T10316	prep	from,obtained
R6544	T10319	T10320	compound	Santa,Cruz
R6545	T10320	T10321	compound	Cruz,Biotechnology
R6546	T10321	T10318	pobj	Biotechnology,from
R6547	T10322	T10316	punct	.,obtained
R6548	T10324	T10325	prep	For,injected
R6549	T10325	T10335	ccomp	injected,dissected
R655	T1436	T1437	det	a,number
R6550	T10326	T10327	compound	BrdU,incorporation
R6551	T10327	T10324	pobj	incorporation,For
R6552	T10328	T10325	punct	", ",injected
R6553	T10329	T10330	amod	timed,mice
R6554	T10330	T10325	nsubjpass	mice,injected
R6555	T10331	T10330	amod	pregnant,mice
R6556	T10332	T10330	prep	at,mice
R6557	T10333	T10332	pobj	E18.5,at
R6558	T10334	T10325	auxpass	were,injected
R6559	T10336	T10325	advmod	intra-peritoneally,injected
R656	T1437	T1431	dobj	number,exhibited
R6560	T10337	T10325	prep	with,injected
R6561	T10338	T10339	nummod	100,μg
R6562	T10339	T10340	nmod	μg,weight
R6563	T10340	T10337	pobj	weight,with
R6564	T10341	T10342	punct	/,gm
R6565	T10342	T10339	prep	gm,μg
R6566	T10343	T10340	compound	body,weight
R6567	T10344	T10340	prep	of,weight
R6568	T10345	T10344	pobj	BrdU,of
R6569	T10346	T10347	punct	(,Sigma
R657	T1438	T1437	prep	of,number
R6570	T10347	T10345	parataxis	Sigma,BrdU
R6571	T10348	T10347	punct	", ",Sigma
R6572	T10349	T10350	compound	St,Louis
R6573	T10350	T10347	npadvmod	Louis,Sigma
R6574	T10351	T10347	punct	", ",Sigma
R6575	T10352	T10347	npadvmod	MO,Sigma
R6576	T10353	T10347	punct	),Sigma
R6577	T10354	T10355	nummod	2,h
R6578	T10355	T10356	npadvmod	h,to
R6579	T10356	T10325	prep	to,injected
R658	T1439	T1440	amod	developmental,abnormalities
R6580	T10357	T10356	amod	prior,to
R6581	T10358	T10356	pobj	sacrifice,to
R6582	T10359	T10335	punct	", ",dissected
R6583	T10360	T10335	nsubj	embryos,dissected
R6584	T10361	T10335	prep	from,dissected
R6585	T10362	T10361	pobj	uteri,from
R6586	T10363	T10335	cc	and,dissected
R6587	T10364	T10335	conj	processed,dissected
R6588	T10365	T10366	mark	as,described
R6589	T10366	T10335	advcl	described,dissected
R659	T1440	T1438	pobj	abnormalities,of
R6590	T10367	T10366	advmod	above,described
R6591	T10368	T10335	punct	.,dissected
R6592	T10370	T10371	nsubjpass	Sections,stained
R6593	T10372	T10371	auxpass	were,stained
R6594	T10373	T10371	prep	with,stained
R6595	T10374	T10375	det	a,antibody
R6596	T10375	T10373	pobj	antibody,with
R6597	T10376	T10375	amod	monoclonal,antibody
R6598	T10377	T10375	amod	anti-BrdU,antibody
R6599	T10378	T10379	punct	(,Bank
R66	T313	T312	prep	of,number
R660	T1441	T1431	cc	and,exhibited
R6600	T10379	T10375	parataxis	Bank,antibody
R6601	T10380	T10379	dep	1,Bank
R6602	T10381	T10382	punct	:,100
R6603	T10382	T10380	prep	100,1
R6604	T10383	T10379	punct	", ",Bank
R6605	T10384	T10385	compound	Developmental,Studies
R6606	T10385	T10379	compound	Studies,Bank
R6607	T10386	T10379	compound	Hybridoma,Bank
R6608	T10387	T10379	punct	", ",Bank
R6609	T10388	T10389	compound	Iowa,City
R661	T1442	T1431	conj	died,exhibited
R6610	T10389	T10379	npadvmod	City,Bank
R6611	T10390	T10379	punct	", ",Bank
R6612	T10391	T10379	npadvmod	IA,Bank
R6613	T10392	T10379	punct	),Bank
R6614	T10393	T10371	cc	and,stained
R6615	T10394	T10371	conj	visualized,stained
R6616	T10395	T10394	prep	with,visualized
R6617	T10396	T10397	det	a,antibody
R6618	T10397	T10395	pobj	antibody,with
R6619	T10398	T10399	npadvmod	Cy2,conjugated
R662	T1443	T1442	prep	within,died
R6620	T10399	T10397	amod	conjugated,antibody
R6621	T10400	T10399	punct	-,conjugated
R6622	T10401	T10397	nmod	rabbit,antibody
R6623	T10402	T10397	amod	anti-mouse,antibody
R6624	T10403	T10394	advcl	using,visualized
R6625	T10404	T10405	det	a,microscope
R6626	T10405	T10403	dobj	microscope,using
R6627	T10406	T10407	npadvmod	laser,scanning
R6628	T10407	T10405	amod	scanning,microscope
R6629	T10408	T10407	punct	-,scanning
R663	T1444	T1445	nummod	24,h
R6630	T10409	T10405	amod	confocal,microscope
R6631	T10410	T10371	punct	.,stained
R6632	T10412	T10413	compound	Cell,death
R6633	T10413	T10414	nsubjpass	death,investigated
R6634	T10415	T10414	auxpass	was,investigated
R6635	T10416	T10414	prep	by,investigated
R6636	T10417	T10418	amod	terminal,end
R6637	T10418	T10416	pobj	end,by
R6638	T10419	T10420	npadvmod	deoxynucleotidyltransferase,mediated
R6639	T10420	T10418	amod	mediated,end
R664	T1445	T1443	pobj	h,within
R6640	T10421	T10420	punct	-,mediated
R6641	T10422	T10418	compound	dUTP,end
R6642	T10423	T10418	compound	nick,end
R6643	T10424	T10418	punct	-,end
R6644	T10425	T10418	amod	labeling,end
R6645	T10426	T10418	punct	(,end
R6646	T10427	T10418	appos	TUNEL,end
R6647	T10428	T10414	punct	),investigated
R6648	T10429	T10414	advcl	using,investigated
R6649	T10430	T10431	det	the,Kit
R665	T1446	T1442	prep	after,died
R6650	T10431	T10429	dobj	Kit,using
R6651	T10432	T10431	nmod	Fluorescein,Kit
R6652	T10433	T10434	advmod	In,Situ
R6653	T10434	T10431	amod	Situ,Kit
R6654	T10435	T10436	compound	Cell,Death
R6655	T10436	T10437	compound	Death,Detection
R6656	T10437	T10431	compound	Detection,Kit
R6657	T10438	T10439	punct	(,Roche
R6658	T10439	T10431	parataxis	Roche,Kit
R6659	T10440	T10439	punct	", ",Roche
R666	T1447	T1446	pobj	birth,after
R6660	T10441	T10439	npadvmod	Indianapolis,Roche
R6661	T10442	T10439	punct	", ",Roche
R6662	T10443	T10439	npadvmod	IN,Roche
R6663	T10444	T10439	punct	),Roche
R6664	T10445	T10429	prep	according,using
R6665	T10446	T10445	prep	to,according
R6666	T10447	T10448	det	the,manufacturer
R6667	T10448	T10449	poss	manufacturer,protocol
R6668	T10449	T10446	pobj	protocol,to
R6669	T10450	T10448	case	's,manufacturer
R667	T1448	T1431	punct	.,exhibited
R6670	T10451	T10414	punct	.,investigated
R6671	T10545	T10546	compound	Western,blotting
R6672	T10548	T10549	compound	Sample,preparation
R6673	T10549	T10550	nsubjpass	preparation,performed
R6674	T10551	T10549	cc	and,preparation
R6675	T10552	T10553	compound	western,blotting
R6676	T10553	T10549	conj	blotting,preparation
R6677	T10554	T10550	auxpass	was,performed
R6678	T10555	T10550	prep	according,performed
R6679	T10556	T10555	prep	to,according
R668	T1450	T1451	det	A,model
R6680	T10557	T10556	pobj	Kawakami,to
R6681	T10558	T10559	advmod	et,al
R6682	T10559	T10557	advmod	al,Kawakami
R6683	T10560	T10550	prep	with,performed
R6684	T10561	T10562	det	some,modifications
R6685	T10562	T10560	pobj	modifications,with
R6686	T10563	T10562	amod	minor,modifications
R6687	T10564	T10565	punct	[,35
R6688	T10565	T10550	parataxis	35,performed
R6689	T10566	T10565	punct	],35
R669	T1451	T1455	nsubjpass	model,reported
R6690	T10567	T10550	punct	.,performed
R6691	T10569	T10570	advmod	Briefly,pooled
R6692	T10571	T10570	punct	", ",pooled
R6693	T10572	T10573	nummod	3,embryos
R6694	T10573	T10570	nsubjpass	embryos,pooled
R6695	T10574	T10573	prep	from,embryos
R6696	T10575	T10576	det	each,genotype
R6697	T10576	T10574	pobj	genotype,from
R6698	T10577	T10570	auxpass	were,pooled
R6699	T10578	T10570	punct	", ",pooled
R67	T314	T315	amod	biochemical,abnormalities
R670	T1452	T1451	amod	similar,model
R6700	T10579	T10570	conj	homogenized,pooled
R6701	T10580	T10579	prep	in,homogenized
R6702	T10581	T10582	npadvmod	ice,cold
R6703	T10582	T10583	amod	cold,buffer
R6704	T10583	T10580	pobj	buffer,in
R6705	T10584	T10583	compound	lysis,buffer
R6706	T10585	T10586	punct	(,pH8.0
R6707	T10586	T10583	parataxis	pH8.0,buffer
R6708	T10587	T10588	nummod	50,mM
R6709	T10588	T10586	nmod	mM,pH8.0
R671	T1453	T1451	compound	mouse,model
R6710	T10589	T10586	nmod	Tris,pH8.0
R6711	T10590	T10586	punct	-,pH8.0
R6712	T10591	T10586	punct	", ",pH8.0
R6713	T10592	T10593	nummod	150,mM
R6714	T10593	T10594	compound	mM,NaCl
R6715	T10594	T10586	conj	NaCl,pH8.0
R6716	T10595	T10594	punct	", ",NaCl
R6717	T10596	T10597	nummod	2,mM
R6718	T10597	T10598	compound	mM,EDTA
R6719	T10598	T10594	conj	EDTA,NaCl
R672	T1454	T1451	compound	SLOS,model
R6720	T10599	T10598	punct	", ",EDTA
R6721	T10600	T10601	nummod	1,%
R6722	T10601	T10602	compound	%,X
R6723	T10602	T10598	conj	X,EDTA
R6724	T10603	T10602	compound	Triton,X
R6725	T10604	T10602	punct	-,X
R6726	T10605	T10602	nummod	100,X
R6727	T10606	T10602	punct	", ",X
R6728	T10607	T10608	nummod	0.5,mM
R6729	T10608	T10609	compound	mM,DTT
R673	T1456	T1451	punct	", ",model
R6730	T10609	T10602	conj	DTT,X
R6731	T10610	T10609	punct	", ",DTT
R6732	T10611	T10612	nummod	1,mM
R6733	T10612	T10613	compound	mM,PMSF
R6734	T10613	T10609	conj	PMSF,DTT
R6735	T10614	T10613	cc	and,PMSF
R6736	T10615	T10616	nummod	2,μg
R6737	T10616	T10613	conj	μg,PMSF
R6738	T10617	T10618	punct	/,ml
R6739	T10618	T10616	prep	ml,μg
R674	T1457	T1451	acl	generated,model
R6740	T10619	T10618	appos	each,ml
R6741	T10620	T10618	prep	of,ml
R6742	T10621	T10620	pobj	aprotinin,of
R6743	T10622	T10621	punct	", ",aprotinin
R6744	T10623	T10621	conj	leupeptin,aprotinin
R6745	T10624	T10623	cc	and,leupeptin
R6746	T10625	T10626	compound	pepstatin,A
R6747	T10626	T10623	conj	A,leupeptin
R6748	T10627	T10586	punct	),pH8.0
R6749	T10628	T10579	prep	with,homogenized
R675	T1458	T1457	prep	by,generated
R6750	T10629	T10630	det	a,homogenizer
R6751	T10630	T10628	pobj	homogenizer,with
R6752	T10631	T10630	compound	tissue,homogenizer
R6753	T10632	T10633	punct	(,Inc.
R6754	T10633	T10630	parataxis	Inc.,homogenizer
R6755	T10634	T10633	compound	PRO,Inc.
R6756	T10635	T10633	compound	Scientific,Inc.
R6757	T10636	T10633	punct	", ",Inc.
R6758	T10637	T10633	npadvmod	Oxford,Inc.
R6759	T10638	T10633	punct	", ",Inc.
R676	T1459	T1458	pcomp	replacing,by
R6760	T10639	T10633	npadvmod	CT,Inc.
R6761	T10640	T10633	punct	),Inc.
R6762	T10641	T10579	advcl	using,homogenized
R6763	T10642	T10643	nummod	three,pulses
R6764	T10643	T10641	dobj	pulses,using
R6765	T10644	T10643	prep	of,pulses
R6766	T10645	T10646	nummod	10,seconds
R6767	T10646	T10644	pobj	seconds,of
R6768	T10647	T10646	appos	each,seconds
R6769	T10648	T10579	cc	and,homogenized
R677	T1460	T1461	nmod	exons,4
R6770	T10649	T10650	advmod	then,subjected
R6771	T10650	T10579	conj	subjected,homogenized
R6772	T10651	T10650	prep	to,subjected
R6773	T10652	T10653	compound	Dounce,homogenizer
R6774	T10653	T10651	pobj	homogenizer,to
R6775	T10654	T10650	prep	by,subjected
R6776	T10655	T10656	nummod	10,strokes
R6777	T10656	T10654	pobj	strokes,by
R6778	T10657	T10570	punct	.,pooled
R6779	T10659	T10660	det	The,homogenates
R678	T1461	T1459	dobj	4,replacing
R6780	T10660	T10661	nsubjpass	homogenates,centrifuged
R6781	T10661	T10663	ccomp	centrifuged,determined
R6782	T10662	T10661	auxpass	were,centrifuged
R6783	T10664	T10661	prep	at,centrifuged
R6784	T10665	T10666	nummod	"10,000",g
R6785	T10666	T10664	pobj	g,at
R6786	T10667	T10661	prep	for,centrifuged
R6787	T10668	T10669	nummod	30,minutes
R6788	T10669	T10667	pobj	minutes,for
R6789	T10670	T10661	prep	at,centrifuged
R679	T1462	T1461	nummod	3,4
R6790	T10671	T10672	nummod	4,°C
R6791	T10672	T10670	pobj	°C,at
R6792	T10673	T10663	punct	", ",determined
R6793	T10674	T10675	nsubj	supernatants,collected
R6794	T10675	T10663	ccomp	collected,determined
R6795	T10676	T10663	punct	", ",determined
R6796	T10677	T10678	compound	protein,concentration
R6797	T10678	T10663	nsubj	concentration,determined
R6798	T10679	T10663	cc	and,determined
R6799	T10680	T10663	conj	used,determined
R68	T315	T313	pobj	abnormalities,of
R680	T1463	T1461	punct	", ",4
R6800	T10681	T10680	prep	for,used
R6801	T10682	T10683	compound	western,blotting
R6802	T10683	T10681	pobj	blotting,for
R6803	T10684	T10663	punct	.,determined
R6804	T10686	T10687	compound	COS1,cells
R6805	T10687	T10688	nsubjpass	cells,transfected
R6806	T10689	T10688	auxpass	were,transfected
R6807	T10690	T10688	advcl	using,transfected
R6808	T10691	T10692	compound	Superfect,reagent
R6809	T10692	T10690	dobj	reagent,using
R681	T1464	T1461	cc	and,4
R6810	T10693	T10692	compound	transfection,reagent
R6811	T10694	T10695	punct	(,Qiagen
R6812	T10695	T10692	parataxis	Qiagen,reagent
R6813	T10696	T10695	punct	", ",Qiagen
R6814	T10697	T10695	npadvmod	Valencia,Qiagen
R6815	T10698	T10695	punct	", ",Qiagen
R6816	T10699	T10695	npadvmod	CA,Qiagen
R6817	T10700	T10695	punct	),Qiagen
R6818	T10701	T10688	prep	with,transfected
R6819	T10702	T10703	compound	expression,constructs
R682	T1465	T1461	conj	part,4
R6820	T10703	T10701	pobj	constructs,with
R6821	T10704	T10703	acl	encoding,constructs
R6822	T10705	T10706	preconj	either,Shh
R6823	T10706	T10704	dobj	Shh,encoding
R6824	T10707	T10706	det	the,Shh
R6825	T10708	T10709	amod	full,length
R6826	T10709	T10706	compound	length,Shh
R6827	T10710	T10709	punct	-,length
R6828	T10711	T10706	cc	or,Shh
R6829	T10712	T10713	det	the,fragment
R683	T1466	T1465	prep	of,part
R6830	T10713	T10706	conj	fragment,Shh
R6831	T10714	T10715	npadvmod	N,terminal
R6832	T10715	T10713	amod	terminal,fragment
R6833	T10716	T10715	punct	-,terminal
R6834	T10717	T10713	prep	of,fragment
R6835	T10718	T10717	pobj	Shh,of
R6836	T10719	T10704	prep	without,encoding
R6837	T10720	T10721	amod	post-translational,modifications
R6838	T10721	T10719	pobj	modifications,without
R6839	T10722	T10723	punct	(,provided
R684	T1467	T1466	pobj	exon,of
R6840	T10723	T10721	parataxis	provided,modifications
R6841	T10724	T10723	nsubj	constructs,provided
R6842	T10725	T10723	advmod	kindly,provided
R6843	T10726	T10723	prep	by,provided
R6844	T10727	T10728	compound	Dr.,Chuang
R6845	T10728	T10726	pobj	Chuang,by
R6846	T10729	T10730	compound	Pao,Tien
R6847	T10730	T10728	compound	Tien,Chuang
R6848	T10731	T10730	punct	-,Tien
R6849	T10732	T10733	punct	", ",Institute
R685	T1468	T1467	nummod	5,exon
R6850	T10733	T10728	parataxis	Institute,Chuang
R6851	T10734	T10735	compound	Cardiovascular,Research
R6852	T10735	T10733	compound	Research,Institute
R6853	T10736	T10733	punct	", ",Institute
R6854	T10737	T10733	npadvmod	University,Institute
R6855	T10738	T10737	prep	of,University
R6856	T10739	T10738	pobj	California,of
R6857	T10740	T10733	punct	", ",Institute
R6858	T10741	T10742	compound	San,Francisco
R6859	T10742	T10733	npadvmod	Francisco,Institute
R686	T1469	T1461	prep	of,4
R6860	T10743	T10733	punct	", ",Institute
R6861	T10744	T10733	npadvmod	CA,Institute
R6862	T10745	T10723	punct	),provided
R6863	T10746	T10703	punct	", ",constructs
R6864	T10747	T10703	acl	harvested,constructs
R6865	T10748	T10749	nummod	3,days
R6866	T10749	T10750	npadvmod	days,after
R6867	T10750	T10747	prep	after,harvested
R6868	T10751	T10752	nmod	transfection,extracts
R6869	T10752	T10750	pobj	extracts,after
R687	T1470	T1471	det	the,gene
R6870	T10753	T10751	cc	and,transfection
R6871	T10754	T10751	conj	protein,transfection
R6872	T10755	T10752	acl	prepared,extracts
R6873	T10756	T10757	mark	as,described
R6874	T10757	T10755	advcl	described,prepared
R6875	T10758	T10757	advmod	above,described
R6876	T10759	T10688	punct	.,transfected
R6877	T10761	T10762	compound	Protein,concentration
R6878	T10762	T10763	nsubjpass	concentration,determined
R6879	T10764	T10763	auxpass	was,determined
R688	T1471	T1469	pobj	gene,of
R6880	T10765	T10763	advcl	using,determined
R6881	T10766	T10767	compound	Bio,Rad
R6882	T10767	T10769	compound	Rad,kit
R6883	T10768	T10767	punct	-,Rad
R6884	T10769	T10765	dobj	kit,using
R6885	T10770	T10769	compound	protein,kit
R6886	T10771	T10769	compound	assay,kit
R6887	T10772	T10773	punct	(,BioRad
R6888	T10773	T10769	parataxis	BioRad,kit
R6889	T10774	T10773	punct	", ",BioRad
R689	T1472	T1471	amod	murine,gene
R6890	T10775	T10773	npadvmod	Hercules,BioRad
R6891	T10776	T10773	punct	", ",BioRad
R6892	T10777	T10773	npadvmod	CA,BioRad
R6893	T10778	T10773	punct	),BioRad
R6894	T10779	T10769	cc	and,kit
R6895	T10780	T10781	nummod	20,μg
R6896	T10781	T10769	conj	μg,kit
R6897	T10782	T10783	punct	(,cells
R6898	T10783	T10781	parataxis	cells,μg
R6899	T10784	T10783	compound	COS1,cells
R69	T316	T314	cc	and,biochemical
R690	T1473	T1471	compound	Dhcr7,gene
R6900	T10785	T10783	punct	),cells
R6901	T10786	T10781	cc	and,μg
R6902	T10787	T10788	nummod	75,μg
R6903	T10788	T10781	conj	μg,μg
R6904	T10789	T10790	punct	(,embryos
R6905	T10790	T10788	parataxis	embryos,μg
R6906	T10791	T10790	punct	),embryos
R6907	T10792	T10781	prep	of,μg
R6908	T10793	T10792	pobj	lysates,of
R6909	T10794	T10781	acl	separated,μg
R691	T1474	T1459	punct	", ",replacing
R6910	T10795	T10794	prep	by,separated
R6911	T10796	T10797	nummod	15,%
R6912	T10797	T10798	compound	%,PAGE
R6913	T10798	T10795	pobj	PAGE,by
R6914	T10799	T10798	compound	SDS,PAGE
R6915	T10800	T10798	punct	-,PAGE
R6916	T10801	T10794	punct	", ",separated
R6917	T10802	T10794	conj	transferred,separated
R6918	T10803	T10802	prep	onto,transferred
R6919	T10804	T10805	compound	nitrocellulose,membrane
R692	T1475	T1459	prep	with,replacing
R6920	T10805	T10803	pobj	membrane,onto
R6921	T10806	T10802	punct	", ",transferred
R6922	T10807	T10802	conj	incubated,transferred
R6923	T10808	T10807	prep	with,incubated
R6924	T10809	T10810	amod	primary,antibody
R6925	T10810	T10808	pobj	antibody,with
R6926	T10811	T10812	punct	(,1
R6927	T10812	T10810	parataxis	1,antibody
R6928	T10813	T10814	compound	Shh,N19
R6929	T10814	T10812	dep	N19,1
R693	T1476	T1477	amod	similar,defects
R6930	T10815	T10814	punct	-,N19
R6931	T10816	T10812	punct	", ",1
R6932	T10817	T10818	punct	:,100
R6933	T10818	T10812	prep	100,1
R6934	T10819	T10812	punct	),1
R6935	T10820	T10807	prep	for,incubated
R6936	T10821	T10822	nummod	2,hours
R6937	T10822	T10820	pobj	hours,for
R6938	T10823	T10807	punct	", ",incubated
R6939	T10824	T10807	advcl	followed,incubated
R694	T1477	T1475	pobj	defects,with
R6940	T10825	T10824	prep	by,followed
R6941	T10826	T10827	nmod	donkey,antibody
R6942	T10827	T10825	pobj	antibody,by
R6943	T10828	T10827	amod	anti-goat,antibody
R6944	T10829	T10827	nmod	HRP,antibody
R6945	T10830	T10827	amod	second,antibody
R6946	T10831	T10832	punct	(,1
R6947	T10832	T10827	parataxis	1,antibody
R6948	T10833	T10834	punct	:,1000
R6949	T10834	T10832	prep	1000,1
R695	T1478	T1477	amod	biochemical,defects
R6950	T10835	T10832	punct	),1
R6951	T10836	T10807	cc	and,incubated
R6952	T10837	T10807	conj	visualized,incubated
R6953	T10838	T10837	prep	by,visualized
R6954	T10839	T10840	amod	chemiluminescent,detection
R6955	T10840	T10838	pobj	detection,by
R6956	T10841	T10837	prep	according,visualized
R6957	T10842	T10841	prep	to,according
R6958	T10843	T10844	poss	manufacturer,protocols
R6959	T10844	T10842	pobj	protocols,to
R696	T1479	T1478	cc	and,biochemical
R6960	T10845	T10843	case	's,manufacturer
R6961	T10846	T10847	punct	(,Sciences
R6962	T10847	T10844	parataxis	Sciences,protocols
R6963	T10848	T10849	compound	Perkin,Elmer
R6964	T10849	T10847	compound	Elmer,Sciences
R6965	T10850	T10849	punct	-,Elmer
R6966	T10851	T10847	compound	Life,Sciences
R6967	T10852	T10847	punct	", ",Sciences
R6968	T10853	T10847	npadvmod	Boston,Sciences
R6969	T10854	T10847	npadvmod	MA,Sciences
R697	T1480	T1478	conj	structural,biochemical
R6970	T10855	T10847	punct	),Sciences
R6971	T10856	T10763	punct	.,determined
R6972	T10893	T10894	compound	Northern,blotting
R6973	T10896	T10897	compound	Northern,blotting
R6974	T10897	T10898	nsubjpass	blotting,performed
R6975	T10899	T10898	auxpass	was,performed
R6976	T10900	T10901	mark	as,described
R6977	T10901	T10898	advcl	described,performed
R6978	T10902	T10901	advmod	previously,described
R6979	T10903	T10904	punct	[,46
R698	T1481	T1477	cc	and,defects
R6980	T10904	T10901	parataxis	46,described
R6981	T10905	T10904	punct	],46
R6982	T10906	T10898	punct	", ",performed
R6983	T10907	T10898	advcl	using,performed
R6984	T10908	T10909	amod	total,RNA
R6985	T10909	T10907	dobj	RNA,using
R6986	T10910	T10911	punct	(,μg
R6987	T10911	T10909	parataxis	μg,RNA
R6988	T10912	T10911	nummod	20,μg
R6989	T10913	T10911	punct	),μg
R699	T1482	T1483	det	a,lethality
R6990	T10914	T10909	prep	from,RNA
R6991	T10915	T10916	compound	P0,mice
R6992	T10916	T10917	compound	mice,lungs
R6993	T10917	T10914	pobj	lungs,from
R6994	T10918	T10898	punct	.,performed
R6995	T10920	T10921	det	The,probes
R6996	T10921	T10923	nsubjpass	probes,provided
R6997	T10922	T10921	compound	cDNA,probes
R6998	T10924	T10921	prep	for,probes
R6999	T10925	T10926	compound	SP,A
R7	T245	T243	prep	in,hypoplasia
R70	T317	T314	conj	structural,biochemical
R700	T1483	T1477	conj	lethality,defects
R7000	T10926	T10924	pobj	A,for
R7001	T10927	T10926	punct	-,A
R7002	T10928	T10926	punct	", ",A
R7003	T10929	T10930	compound	SP,B
R7004	T10930	T10926	conj	B,A
R7005	T10931	T10930	punct	-,B
R7006	T10932	T10930	cc	and,B
R7007	T10933	T10934	compound	SP,C
R7008	T10934	T10930	conj	C,B
R7009	T10935	T10934	punct	-,C
R701	T1484	T1485	nummod	100,%
R7010	T10936	T10923	auxpass	were,provided
R7011	T10937	T10923	advmod	kindly,provided
R7012	T10938	T10923	agent	by,provided
R7013	T10939	T10940	compound	Dr.,Whitsett
R7014	T10940	T10938	pobj	Whitsett,by
R7015	T10941	T10940	compound	Jeffery,Whitsett
R7016	T10942	T10943	punct	(,Division
R7017	T10943	T10940	parataxis	Division,Whitsett
R7018	T10944	T10943	prep	of,Division
R7019	T10945	T10946	compound	Pulmonary,Biology
R702	T1485	T1483	nmod	%,lethality
R7020	T10946	T10944	pobj	Biology,of
R7021	T10947	T10943	punct	", ",Division
R7022	T10948	T10949	nmod	Cincinnati,Hospital
R7023	T10949	T10952	compound	Hospital,Center
R7024	T10950	T10949	poss	Children,Hospital
R7025	T10951	T10950	case	's,Children
R7026	T10952	T10943	npadvmod	Center,Division
R7027	T10953	T10952	compound	Medical,Center
R7028	T10954	T10943	punct	", ",Division
R7029	T10955	T10943	npadvmod	Cincinnati,Division
R703	T1486	T1483	amod	neonatal,lethality
R7030	T10956	T10943	punct	", ",Division
R7031	T10957	T10943	npadvmod	Ohio,Division
R7032	T10958	T10943	punct	),Division
R7033	T10959	T10923	punct	.,provided
R7034	T10961	T10962	det	A,probe
R7035	T10962	T10964	nsubjpass	probe,amplified
R7036	T10963	T10962	compound	cDNA,probe
R7037	T10965	T10962	prep	for,probe
R7038	T10966	T10967	compound	SP,D
R7039	T10967	T10965	pobj	D,for
R704	T1487	T1455	punct	", ",reported
R7040	T10968	T10967	punct	-,D
R7041	T10969	T10964	auxpass	was,amplified
R7042	T10970	T10964	prep	by,amplified
R7043	T10971	T10972	compound	RT,PCR
R7044	T10972	T10970	pobj	PCR,by
R7045	T10973	T10972	punct	-,PCR
R7046	T10974	T10964	punct	", ",amplified
R7047	T10975	T10964	advcl	using,amplified
R7048	T10976	T10977	amod	murine,primers
R7049	T10977	T10975	dobj	primers,using
R705	T1488	T1455	aux	has,reported
R7050	T10978	T10979	nmod	SP,D
R7051	T10979	T10977	nmod	D,primers
R7052	T10980	T10979	punct	-,D
R7053	T10981	T10982	npadvmod	cDNA,specific
R7054	T10982	T10977	amod	specific,primers
R7055	T10983	T10984	punct	(,TGCCTGGTCGTGATGGACG
R7056	T10984	T10977	parataxis	TGCCTGGTCGTGATGGACG,primers
R7057	T10985	T10984	nmod	sense,TGCCTGGTCGTGATGGACG
R7058	T10986	T10984	nummod	5,TGCCTGGTCGTGATGGACG
R7059	T10987	T10986	punct	',5
R706	T1489	T1455	advmod	also,reported
R7060	T10988	T10984	punct	-,TGCCTGGTCGTGATGGACG
R7061	T10989	T10984	punct	-,TGCCTGGTCGTGATGGACG
R7062	T10990	T10984	nummod	3,TGCCTGGTCGTGATGGACG
R7063	T10991	T10984	punct	',TGCCTGGTCGTGATGGACG
R7064	T10992	T10984	punct	", ",TGCCTGGTCGTGATGGACG
R7065	T10993	T10984	cc	and,TGCCTGGTCGTGATGGACG
R7066	T10994	T10995	amod	antisense,AGCACTGTCTGGAAGCCCGC
R7067	T10995	T10984	conj	AGCACTGTCTGGAAGCCCGC,TGCCTGGTCGTGATGGACG
R7068	T10996	T10995	nummod	5,AGCACTGTCTGGAAGCCCGC
R7069	T10997	T10996	punct	',5
R707	T1490	T1455	auxpass	been,reported
R7070	T10998	T10995	punct	-,AGCACTGTCTGGAAGCCCGC
R7071	T10999	T10995	punct	-,AGCACTGTCTGGAAGCCCGC
R7072	T11000	T10995	nummod	3,AGCACTGTCTGGAAGCCCGC
R7073	T11001	T10995	punct	',AGCACTGTCTGGAAGCCCGC
R7074	T11002	T10984	punct	),TGCCTGGTCGTGATGGACG
R7075	T11003	T10964	cc	and,amplified
R7076	T11004	T10964	conj	confirmed,amplified
R7077	T11005	T11004	prep	by,confirmed
R7078	T11006	T11005	pobj	sequencing,by
R7079	T11007	T10964	punct	.,amplified
R708	T1491	T1492	punct	[,17
R7080	T11053	T11054	amod	Body,length
R7081	T11054	T11055	compound	length,curves
R7082	T11056	T11055	compound	growth,curves
R7083	T11057	T11055	punct	.,curves
R7084	T11059	T11060	amod	Body,length
R7085	T11060	T11061	compound	length,growth
R7086	T11061	T11062	nsubj	growth,is
R7087	T11063	T11061	cc	vs.,growth
R7088	T11064	T11065	amod	gestational,days
R7089	T11065	T11061	conj	days,growth
R709	T1492	T1455	parataxis	17,reported
R7090	T11066	T11067	mark	as,shown
R7091	T11067	T11062	advcl	shown,is
R7092	T11068	T11062	punct	.,is
R7093	T11070	T11071	compound	Control,embryos
R7094	T11071	T11072	nsubj	embryos,were
R7095	T11073	T11074	punct	(,triangles
R7096	T11074	T11071	parataxis	triangles,embryos
R7097	T11075	T11074	amod	filled,triangles
R7098	T11076	T11074	punct	", ",triangles
R7099	T11077	T11078	amod	wild,type
R71	T318	T310	punct	", ",is
R710	T1493	T1492	punct	],17
R7100	T11078	T11080	nmod	type,embryos
R7101	T11079	T11078	punct	-,type
R7102	T11080	T11083	nsubjpass	embryos,pooled
R7103	T11081	T11078	cc	and,type
R7104	T11082	T11078	conj	heterozygous,type
R7105	T11083	T11074	ccomp	pooled,triangles
R7106	T11084	T11083	auxpass	were,pooled
R7107	T11085	T11083	advmod	together,pooled
R7108	T11086	T11087	mark	as,were
R7109	T11087	T11083	advcl	were,pooled
R711	T1494	T1455	punct	.,reported
R7110	T11088	T11087	expl	there,were
R7111	T11089	T11090	det	no,differences
R7112	T11090	T11087	attr	differences,were
R7113	T11091	T11087	prep	between,were
R7114	T11092	T11093	det	these,groups
R7115	T11093	T11091	pobj	groups,between
R7116	T11094	T11093	nummod	two,groups
R7117	T11095	T11074	punct	),triangles
R7118	T11096	T11071	cc	and,embryos
R7119	T11097	T11098	compound	knockout,embryos
R712	T1496	T1497	amod	Poor,development
R7120	T11098	T11071	conj	embryos,embryos
R7121	T11099	T11100	punct	(,circles
R7122	T11100	T11098	parataxis	circles,embryos
R7123	T11101	T11100	amod	filled,circles
R7124	T11102	T11100	punct	),circles
R7125	T11103	T11104	mark	as,shown
R7126	T11104	T11072	advcl	shown,were
R7127	T11105	T11072	punct	.,were
R7128	T11107	T11108	det	The,curve
R7129	T11108	T11110	nsubj	curve,showed
R713	T1497	T1499	nsubj	development,are
R7130	T11109	T11108	compound	growth,curve
R7131	T11111	T11112	det	a,increase
R7132	T11112	T11110	dobj	increase,showed
R7133	T11113	T11112	amod	linear,increase
R7134	T11114	T11110	prep	from,showed
R7135	T11115	T11114	pobj	E9.5,from
R7136	T11116	T11114	prep	to,from
R7137	T11117	T11116	pobj	E19.5,to
R7138	T11118	T11110	prep	in,showed
R7139	T11119	T11118	pobj	controls,in
R714	T1498	T1497	compound	lung,development
R7140	T11120	T11110	punct	", ",showed
R7141	T11121	T11110	cc	but,showed
R7142	T11122	T11110	conj	deviated,showed
R7143	T11123	T11122	prep	at,deviated
R7144	T11124	T11125	punct	~,E14.5
R7145	T11125	T11123	pobj	E14.5,at
R7146	T11126	T11122	prep	in,deviated
R7147	T11127	T11128	nmod	Dhcr7,embryos
R7148	T11128	T11126	pobj	embryos,in
R7149	T11129	T11127	punct	-,Dhcr7
R715	T1500	T1497	cc	or,development
R7150	T11130	T11127	punct	/,Dhcr7
R7151	T11131	T11127	punct	-,Dhcr7
R7152	T11132	T11110	punct	.,showed
R7153	T11134	T11135	compound	Body,lengths
R7154	T11135	T11136	nsubjpass	lengths,decreased
R7155	T11137	T11135	prep	at,lengths
R7156	T11138	T11137	pobj	E17.5,at
R7157	T11139	T11140	punct	(,mm
R7158	T11140	T11138	parataxis	mm,E17.5
R7159	T11141	T11142	quantmod	18.87,1.29
R716	T1501	T1502	amod	diffuse,atelectasis
R7160	T11142	T11140	nummod	1.29,mm
R7161	T11143	T11142	punct	±,1.29
R7162	T11144	T11140	prep	in,mm
R7163	T11145	T11144	pobj	controls,in
R7164	T11146	T11140	punct	", ",mm
R7165	T11147	T11148	nsubj	n,mm
R7166	T11148	T11140	ccomp	mm,mm
R7167	T11149	T11148	punct	=,mm
R7168	T11150	T11148	nummod	15,mm
R7169	T11151	T11150	cc	vs.,15
R717	T1502	T1497	conj	atelectasis,development
R7170	T11152	T11153	quantmod	16.69,1.05
R7171	T11153	T11150	conj	1.05,15
R7172	T11154	T11153	punct	±,1.05
R7173	T11155	T11148	prep	in,mm
R7174	T11156	T11155	pobj	Dhcr7,in
R7175	T11157	T11156	punct	-,Dhcr7
R7176	T11158	T11156	punct	/,Dhcr7
R7177	T11159	T11156	punct	-,Dhcr7
R7178	T11160	T11140	punct	", ",mm
R7179	T11161	T11162	nsubj	n,7
R718	T1503	T1502	nmod	lung,atelectasis
R7180	T11162	T11140	ccomp	7,mm
R7181	T11163	T11162	punct	=,7
R7182	T11164	T11140	punct	", ",mm
R7183	T11165	T11166	nsubj	p,0.05
R7184	T11166	T11140	ccomp	0.05,mm
R7185	T11167	T11166	punct	<,0.05
R7186	T11168	T11140	punct	),mm
R7187	T11169	T11138	punct	", ",E17.5
R7188	T11170	T11138	appos	E18.5,E17.5
R7189	T11171	T11172	punct	(,mm
R719	T1504	T1502	amod	alveolar,atelectasis
R7190	T11172	T11170	parataxis	mm,E18.5
R7191	T11173	T11174	quantmod	21.35,1.05
R7192	T11174	T11172	nummod	1.05,mm
R7193	T11175	T11174	punct	±,1.05
R7194	T11176	T11172	prep	in,mm
R7195	T11177	T11176	pobj	controls,in
R7196	T11178	T11172	punct	", ",mm
R7197	T11179	T11180	nsubj	n,mm
R7198	T11180	T11172	ccomp	mm,mm
R7199	T11181	T11180	punct	=,mm
R72	T319	T320	nummod	one,cause
R720	T1505	T1502	prep	with,atelectasis
R7200	T11182	T11180	nummod	15,mm
R7201	T11183	T11182	cc	vs.,15
R7202	T11184	T11185	quantmod	19.38,0.9
R7203	T11185	T11182	conj	0.9,15
R7204	T11186	T11185	punct	±,0.9
R7205	T11187	T11180	prep	in,mm
R7206	T11188	T11187	pobj	Dhcr7,in
R7207	T11189	T11188	punct	-,Dhcr7
R7208	T11190	T11188	punct	/,Dhcr7
R7209	T11191	T11188	punct	-,Dhcr7
R721	T1506	T1507	amod	cyanotic,breathing
R7210	T11192	T11172	punct	", ",mm
R7211	T11193	T11194	nsubj	n,5
R7212	T11194	T11172	ccomp	5,mm
R7213	T11195	T11194	punct	=,5
R7214	T11196	T11172	punct	", ",mm
R7215	T11197	T11198	nsubj	p,0.05
R7216	T11198	T11172	ccomp	0.05,mm
R7217	T11199	T11198	punct	<,0.05
R7218	T11200	T11172	punct	),mm
R7219	T11201	T11170	cc	and,E18.5
R722	T1507	T1505	pobj	breathing,with
R7220	T11202	T11170	conj	E19.5,E18.5
R7221	T11203	T11204	punct	(,mm
R7222	T11204	T11202	parataxis	mm,E19.5
R7223	T11205	T11206	quantmod	23.4,0.97
R7224	T11206	T11204	nummod	0.97,mm
R7225	T11207	T11206	punct	±,0.97
R7226	T11208	T11204	prep	in,mm
R7227	T11209	T11208	pobj	controls,in
R7228	T11210	T11204	punct	", ",mm
R7229	T11211	T11212	nsubj	n,mm
R723	T1508	T1506	cc	and,cyanotic
R7230	T11212	T11204	ccomp	mm,mm
R7231	T11213	T11212	punct	=,mm
R7232	T11214	T11212	nummod	13,mm
R7233	T11215	T11214	cc	vs.,13
R7234	T11216	T11217	quantmod	20.3,0.44
R7235	T11217	T11214	conj	0.44,13
R7236	T11218	T11217	punct	±,0.44
R7237	T11219	T11212	prep	in,mm
R7238	T11220	T11219	pobj	Dhcr7,in
R7239	T11221	T11220	punct	-,Dhcr7
R724	T1509	T1506	conj	lethargic,cyanotic
R7240	T11222	T11220	punct	/,Dhcr7
R7241	T11223	T11220	punct	-,Dhcr7
R7242	T11224	T11204	punct	", ",mm
R7243	T11225	T11226	nsubj	n,4
R7244	T11226	T11204	ccomp	4,mm
R7245	T11227	T11226	punct	=,4
R7246	T11228	T11204	punct	", ",mm
R7247	T11229	T11230	nsubj	p,0.05
R7248	T11230	T11204	ccomp	0.05,mm
R7249	T11231	T11230	punct	<,0.05
R725	T1510	T1499	acomp	consistent,are
R7250	T11232	T11204	punct	),mm
R7251	T11233	T11136	auxpass	were,decreased
R7252	T11234	T11136	advmod	significantly,decreased
R7253	T11235	T11136	punct	", ",decreased
R7254	T11236	T11136	prep	compared,decreased
R7255	T11237	T11236	prep	to,compared
R7256	T11238	T11237	pobj	those,to
R7257	T11239	T11238	prep	in,those
R7258	T11240	T11239	pobj	controls,in
R7259	T11241	T11136	punct	.,decreased
R726	T1511	T1499	prep	between,are
R7260	T11243	T11244	nsubjpass	Results,expressed
R7261	T11245	T11244	auxpass	are,expressed
R7262	T11246	T11244	prep	as,expressed
R7263	T11247	T11248	nmod	mean,s.d
R7264	T11248	T11246	pobj	s.d,as
R7265	T11249	T11248	punct	±,s.d
R7266	T11250	T11244	punct	.,expressed
R7267	T11316	T11317	amod	Embryonic,size
R7268	T11318	T11317	punct	", ",size
R7269	T11319	T11320	compound	lung,morphology
R727	T1512	T1513	preconj	both,models
R7270	T11320	T11317	appos	morphology,size
R7271	T11321	T11320	cc	and,morphology
R7272	T11322	T11320	conj	histology,morphology
R7273	T11323	T11317	prep	at,size
R7274	T11324	T11325	amod	various,stages
R7275	T11325	T11323	pobj	stages,at
R7276	T11326	T11325	prep	of,stages
R7277	T11327	T11326	pobj	development,of
R7278	T11328	T11317	punct	.,size
R7279	T11330	T11331	compound	Panel,A
R728	T1513	T1511	pobj	models,between
R7280	T11331	T11332	nsubj	A,shows
R7281	T11333	T11334	det	the,size
R7282	T11334	T11332	dobj	size,shows
R7283	T11335	T11334	cc	and,size
R7284	T11336	T11337	amod	general,morphology
R7285	T11337	T11334	conj	morphology,size
R7286	T11338	T11334	prep	of,size
R7287	T11339	T11340	amod	wild,type
R7288	T11340	T11342	nmod	type,embryos
R7289	T11341	T11340	punct	-,type
R729	T1514	T1515	npadvmod	Dhcr7,null
R7290	T11342	T11338	pobj	embryos,of
R7291	T11343	T11340	punct	(,type
R7292	T11344	T11345	punct	+,+
R7293	T11345	T11340	appos	+,type
R7294	T11346	T11345	punct	/,+
R7295	T11347	T11340	punct	),type
R7296	T11348	T11340	cc	and,type
R7297	T11349	T11340	conj	Dhcr7,type
R7298	T11350	T11349	punct	-,Dhcr7
R7299	T11351	T11349	punct	/,Dhcr7
R73	T320	T310	nsubj	cause,is
R730	T1515	T1513	amod	null,models
R7300	T11352	T11349	punct	-,Dhcr7
R7301	T11353	T11349	punct	(,Dhcr7
R7302	T11354	T11355	punct	-,-
R7303	T11355	T11349	appos	-,Dhcr7
R7304	T11356	T11355	punct	/,-
R7305	T11357	T11349	punct	),Dhcr7
R7306	T11358	T11334	prep	at,size
R7307	T11359	T11358	pobj	E10.5,at
R7308	T11360	T11334	punct	", ",size
R7309	T11361	T11334	prep	without,size
R731	T1516	T1517	punct	[,17
R7310	T11362	T11363	amod	significant,difference
R7311	T11363	T11361	pobj	difference,without
R7312	T11364	T11332	punct	.,shows
R7313	T11366	T11367	prep	At,were
R7314	T11368	T11366	pobj	P0,At
R7315	T11369	T11367	punct	", ",were
R7316	T11370	T11371	det	the,pups
R7317	T11371	T11367	nsubj	pups,were
R7318	T11372	T11371	compound	knockout,pups
R7319	T11373	T11374	advmod	significantly,smaller
R732	T1517	T1499	parataxis	17,are
R7320	T11374	T11367	acomp	smaller,were
R7321	T11375	T11367	cc	and,were
R7322	T11376	T11367	conj	appeared,were
R7323	T11377	T11376	oprd	hypoxic,appeared
R7324	T11378	T11379	punct	(,B
R7325	T11379	T11376	parataxis	B,appeared
R7326	T11380	T11379	compound	panel,B
R7327	T11381	T11379	punct	),B
R7328	T11382	T11367	punct	.,were
R7329	T11384	T11385	compound	Panel,C
R733	T1518	T1517	nummod	16,17
R7330	T11385	T11386	nsubj	C,shows
R7331	T11387	T11388	amod	dorsal,views
R7332	T11388	T11386	dobj	views,shows
R7333	T11389	T11388	prep	of,views
R7334	T11390	T11389	pobj	lungs,of
R7335	T11391	T11390	prep	from,lungs
R7336	T11392	T11391	pobj	knockout,from
R7337	T11393	T11392	cc	and,knockout
R7338	T11394	T11395	amod	wild,type
R7339	T11395	T11392	conj	type,knockout
R734	T1519	T1517	punct	",",17
R7340	T11396	T11395	punct	-,type
R7341	T11397	T11392	prep	at,knockout
R7342	T11398	T11397	pobj	E13.5,at
R7343	T11399	T11386	cc	and,shows
R7344	T11400	T11401	compound	panel,D
R7345	T11401	T11402	nsubj	D,those
R7346	T11402	T11386	conj	those,shows
R7347	T11403	T11402	prep	at,those
R7348	T11404	T11403	pobj	P0,at
R7349	T11405	T11402	punct	.,those
R735	T1520	T1517	punct	],17
R7350	T11407	T11408	amod	Cross,sectional
R7351	T11408	T11410	amod	sectional,examination
R7352	T11409	T11408	punct	-,sectional
R7353	T11410	T11411	nsubj	examination,showed
R7354	T11412	T11410	prep	at,examination
R7355	T11413	T11414	det	the,level
R7356	T11414	T11412	pobj	level,at
R7357	T11415	T11414	amod	cardiopulmonary,level
R7358	T11416	T11410	prep	at,examination
R7359	T11417	T11416	pobj	E,at
R736	T1521	T1499	punct	", ",are
R7360	T11418	T11417	nummod	20.5,E
R7361	T11419	T11420	punct	(,to
R7362	T11420	T11417	parataxis	to,E
R7363	T11421	T11420	amod	prior,to
R7364	T11422	T11420	pobj	birth,to
R7365	T11423	T11420	punct	),to
R7366	T11424	T11425	amod	normal,septation
R7367	T11425	T11411	dobj	septation,showed
R7368	T11426	T11425	compound	lobar,septation
R7369	T11427	T11411	punct	", ",showed
R737	T1522	T1499	cc	and,are
R7370	T11428	T11411	cc	but,showed
R7371	T11429	T11430	nsubj	lungs,were
R7372	T11430	T11411	conj	were,showed
R7373	T11431	T11429	prep	from,lungs
R7374	T11432	T11433	compound	knockout,animals
R7375	T11433	T11431	pobj	animals,from
R7376	T11434	T11430	acomp	smaller,were
R7377	T11435	T11434	prep	in,smaller
R7378	T11436	T11435	pobj	size,in
R7379	T11437	T11430	cc	and,were
R738	T1523	T1524	advmod	therefore,is
R7380	T11438	T11430	conj	had,were
R7381	T11439	T11440	amod	less,distal
R7382	T11440	T11441	amod	distal,space
R7383	T11441	T11438	dobj	space,had
R7384	T11442	T11441	compound	sac,space
R7385	T11443	T11444	punct	(,E
R7386	T11444	T11438	parataxis	E,had
R7387	T11445	T11444	advmod	cf.,E
R7388	T11446	T11444	compound	panels,E
R7389	T11447	T11444	cc	and,E
R739	T1524	T1499	conj	is,are
R7390	T11448	T11444	conj	F,E
R7391	T11449	T11444	punct	),E
R7392	T11450	T11430	punct	.,were
R7393	T11452	T11453	amod	Original,magnification
R7394	T11454	T11453	punct	: ,magnification
R7395	T11455	T11456	compound	Panels,A
R7396	T11456	T11457	dep	A,2X
R7397	T11457	T11461	dep	2X,2X
R7398	T11458	T11456	cc	and,A
R7399	T11459	T11456	conj	C,A
R74	T321	T320	prep	of,cause
R740	T1525	T1526	det	the,reason
R7400	T11460	T11457	punct	", ",2X
R7401	T11461	T11453	dep	2X,magnification
R7402	T11462	T11461	punct	;,2X
R7403	T11463	T11464	compound	panels,B
R7404	T11464	T11465	dep	B,0.7X
R7405	T11465	T11461	dep	0.7X,2X
R7406	T11466	T11464	cc	and,B
R7407	T11467	T11464	conj	D,B
R7408	T11468	T11465	punct	", ",0.7X
R7409	T11469	T11461	punct	;,2X
R741	T1526	T1524	attr	reason,is
R7410	T11470	T11461	dep	E,2X
R7411	T11471	T11470	cc	and,E
R7412	T11472	T11470	conj	F,E
R7413	T11473	T11461	punct	", ",2X
R7414	T11474	T11453	punct	.,magnification
R7415	T11476	T11477	dep	Labels,r
R7416	T11478	T11477	punct	: ,r
R7417	T11479	T11477	punct	", ",r
R7418	T11480	T11477	appos	right,r
R7419	T11481	T11477	punct	;,r
R742	T1527	T1526	amod	likely,reason
R7420	T11482	T11477	appos	l,r
R7421	T11483	T11482	punct	", ",l
R7422	T11484	T11482	appos	left,l
R7423	T11485	T11477	punct	;,r
R7424	T11486	T11477	appos	b,r
R7425	T11487	T11486	punct	", ",b
R7426	T11488	T11486	appos	bronchial,b
R7427	T11489	T11477	punct	;,r
R7428	T11490	T11477	appos	br,r
R7429	T11491	T11490	punct	", ",br
R743	T1528	T1526	prep	for,reason
R7430	T11492	T11490	appos	bronchiole,br
R7431	T11493	T11477	punct	;,r
R7432	T11494	T11477	appos	e,r
R7433	T11495	T11494	punct	", ",e
R7434	T11496	T11494	appos	esophagus,e
R7435	T11497	T11477	punct	;,r
R7436	T11498	T11477	appos	pa,r
R7437	T11499	T11498	punct	", ",pa
R7438	T11500	T11501	amod	pulmonary,artery
R7439	T11501	T11498	appos	artery,pa
R744	T1529	T1530	det	the,insufficiency
R7440	T11502	T11477	punct	;,r
R7441	T11503	T11477	appos	ra,r
R7442	T11504	T11503	punct	", ",ra
R7443	T11505	T11506	amod	right,atrium
R7444	T11506	T11503	appos	atrium,ra
R7445	T11507	T11477	punct	;,r
R7446	T11508	T11477	appos	la,r
R7447	T11509	T11508	punct	", ",la
R7448	T11510	T11511	amod	left,atrium
R7449	T11511	T11508	appos	atrium,la
R745	T1530	T1528	pobj	insufficiency,for
R7450	T11512	T11477	punct	;,r
R7451	T11513	T11477	appos	rv,r
R7452	T11514	T11513	punct	", ",rv
R7453	T11515	T11516	amod	right,ventricle
R7454	T11516	T11513	appos	ventricle,rv
R7455	T11517	T11477	punct	;,r
R7456	T11518	T11477	appos	lv,r
R7457	T11519	T11518	punct	", ",lv
R7458	T11520	T11521	amod	left,ventricle
R7459	T11521	T11518	appos	ventricle,lv
R746	T1531	T1530	amod	respiratory,insufficiency
R7460	T11522	T11477	punct	.,r
R7461	T11605	T11606	amod	Histological,analyses
R7462	T11607	T11606	prep	of,analyses
R7463	T11608	T11607	pobj	lungs,of
R7464	T11609	T11608	prep	at,lungs
R7465	T11610	T11611	amod	various,stages
R7466	T11611	T11609	pobj	stages,at
R7467	T11612	T11611	amod	embryonic,stages
R7468	T11613	T11606	punct	.,analyses
R7469	T11615	T11616	compound	Panel,A
R747	T1532	T1530	cc	and,insufficiency
R7470	T11616	T11617	nsubj	A,indicates
R7471	T11618	T11619	compound	lung,sections
R7472	T11619	T11617	dobj	sections,indicates
R7473	T11620	T11619	acl	stained,sections
R7474	T11621	T11620	prep	with,stained
R7475	T11622	T11621	pobj	H,with
R7476	T11623	T11622	cc	and,H
R7477	T11624	T11622	conj	E,H
R7478	T11625	T11620	punct	", ",stained
R7479	T11626	T11620	cc	and,stained
R748	T1533	T1534	amod	subsequent,death
R7480	T11627	T11620	conj	taken,stained
R7481	T11628	T11627	prep	at,taken
R7482	T11629	T11630	amod	various,stages
R7483	T11630	T11628	pobj	stages,at
R7484	T11631	T11630	amod	gestational,stages
R7485	T11632	T11633	mark	as,indicated
R7486	T11633	T11620	advcl	indicated,stained
R7487	T11634	T11617	punct	.,indicates
R7488	T11636	T11637	det	The,columns
R7489	T11637	T11640	nsubj	columns,are
R749	T1534	T1530	conj	death,insufficiency
R7490	T11638	T11637	amod	first,columns
R7491	T11639	T11637	nummod	two,columns
R7492	T11641	T11640	prep	at,are
R7493	T11642	T11643	amod	lower,magnification
R7494	T11643	T11641	pobj	magnification,at
R7495	T11644	T11643	punct	(,magnification
R7496	T11645	T11643	appos	10X,magnification
R7497	T11646	T11643	punct	),magnification
R7498	T11647	T11640	cc	and,are
R7499	T11648	T11649	det	the,columns
R75	T322	T321	pobj	death,of
R750	T1535	T1530	prep	of,insufficiency
R7500	T11649	T11652	nsubj	columns,at
R7501	T11650	T11649	amod	last,columns
R7502	T11651	T11649	nummod	two,columns
R7503	T11652	T11640	conj	at,are
R7504	T11653	T11654	amod	higher,magnification
R7505	T11654	T11652	pobj	magnification,at
R7506	T11655	T11654	punct	(,magnification
R7507	T11656	T11654	appos	40X,magnification
R7508	T11657	T11654	punct	),magnification
R7509	T11658	T11652	punct	.,at
R751	T1536	T1537	nmod	Dhcr7,pups
R7510	T11660	T11661	det	The,columns
R7511	T11661	T11665	nsubj	columns,are
R7512	T11662	T11661	amod	first,columns
R7513	T11663	T11662	cc	and,first
R7514	T11664	T11662	conj	third,first
R7515	T11666	T11667	amod	representative,sections
R7516	T11667	T11665	attr	sections,are
R7517	T11668	T11667	prep	from,sections
R7518	T11669	T11670	amod	wild,type
R7519	T11670	T11672	nmod	type,embryos
R752	T1537	T1535	pobj	pups,of
R7520	T11671	T11670	punct	-,type
R7521	T11672	T11668	pobj	embryos,from
R7522	T11673	T11670	punct	(,type
R7523	T11674	T11675	punct	+,+
R7524	T11675	T11670	appos	+,type
R7525	T11676	T11675	punct	/,+
R7526	T11677	T11670	punct	),type
R7527	T11678	T11665	cc	and,are
R7528	T11679	T11680	det	the,columns
R7529	T11680	T11684	nsubj	columns,from
R753	T1538	T1536	punct	-,Dhcr7
R7530	T11681	T11680	amod	second,columns
R7531	T11682	T11681	cc	and,second
R7532	T11683	T11681	conj	fourth,second
R7533	T11684	T11665	conj	from,are
R7534	T11685	T11686	nmod	knockout,embryos
R7535	T11686	T11684	pobj	embryos,from
R7536	T11687	T11685	punct	(,knockout
R7537	T11688	T11689	punct	-,-
R7538	T11689	T11685	appos	-,knockout
R7539	T11690	T11689	punct	/,-
R754	T1539	T1536	punct	/,Dhcr7
R7540	T11691	T11686	punct	),embryos
R7541	T11692	T11684	punct	.,from
R7542	T11694	T11695	det	No,differences
R7543	T11695	T11696	nsubj	differences,were
R7544	T11697	T11696	acomp	visible,were
R7545	T11698	T11696	prep	at,were
R7546	T11699	T11698	pobj	E14.5,at
R7547	T11700	T11699	cc	or,E14.5
R7548	T11701	T11699	conj	E16.5,E14.5
R7549	T11702	T11703	punct	(,A
R755	T1540	T1536	punct	-,Dhcr7
R7550	T11703	T11696	parataxis	A,were
R7551	T11704	T11703	compound	panel,A
R7552	T11705	T11703	punct	", ",A
R7553	T11706	T11703	meta	a,A
R7554	T11707	T11706	punct	-,a
R7555	T11708	T11706	dep	h,a
R7556	T11709	T11703	punct	),A
R7557	T11710	T11696	punct	.,were
R7558	T11712	T11713	amod	Significant,differences
R7559	T11713	T11714	nsubj	differences,were
R756	T1541	T1499	punct	.,are
R7560	T11715	T11714	acomp	visible,were
R7561	T11716	T11714	prep	from,were
R7562	T11717	T11716	pobj	E17.5,from
R7563	T11718	T11717	advmod	onwards,E17.5
R7564	T11719	T11720	punct	(,A
R7565	T11720	T11714	parataxis	A,were
R7566	T11721	T11720	compound	panel,A
R7567	T11722	T11720	punct	", ",A
R7568	T11723	T11720	meta	i,A
R7569	T11724	T11723	punct	-,i
R757	T1543	T1544	det	These,mice
R7570	T11725	T11723	dep	x,i
R7571	T11726	T11720	punct	),A
R7572	T11727	T11714	punct	.,were
R7573	T11729	T11730	amod	Distal,tubules
R7574	T11730	T11731	nsubj	tubules,showed
R7575	T11732	T11731	dobj	dilation,showed
R7576	T11733	T11732	cc	and,dilation
R7577	T11734	T11735	compound	mesenchyme,thinning
R7578	T11735	T11732	conj	thinning,dilation
R7579	T11736	T11732	punct	", ",dilation
R758	T1544	T1547	nsubj	mice,are
R7580	T11737	T11732	prep	with,dilation
R7581	T11738	T11737	pobj	progression,with
R7582	T11739	T11738	prep	of,progression
R7583	T11740	T11739	pobj	septation,of
R7584	T11741	T11731	prep	in,showed
R7585	T11742	T11743	amod	wild,type
R7586	T11743	T11745	compound	type,lungs
R7587	T11744	T11743	punct	-,type
R7588	T11745	T11741	pobj	lungs,in
R7589	T11746	T11747	punct	(,A
R759	T1545	T1546	compound	Dhcr7,knockout
R7590	T11747	T11731	parataxis	A,showed
R7591	T11748	T11747	compound	panel,A
R7592	T11749	T11747	punct	", ",A
R7593	T11750	T11747	meta	i,A
R7594	T11751	T11750	punct	", ",i
R7595	T11752	T11750	conj	m,i
R7596	T11753	T11752	cc	and,m
R7597	T11754	T11752	conj	q,m
R7598	T11755	T11747	punct	),A
R7599	T11756	T11731	punct	", ",showed
R76	T323	T310	prep	from,is
R760	T1546	T1544	compound	knockout,mice
R7600	T11757	T11758	mark	whereas,showed
R7601	T11758	T11731	advcl	showed,showed
R7602	T11759	T11760	det	the,lungs
R7603	T11760	T11758	nsubj	lungs,showed
R7604	T11761	T11760	compound	knockout,lungs
R7605	T11762	T11763	advmod	less,saccular
R7606	T11763	T11764	amod	saccular,structures
R7607	T11764	T11758	dobj	structures,showed
R7608	T11765	T11764	cc	and,structures
R7609	T11766	T11767	amod	more,mesenchyme
R761	T1548	T1547	attr	models,are
R7610	T11767	T11764	conj	mesenchyme,structures
R7611	T11768	T11769	punct	(,A
R7612	T11769	T11758	parataxis	A,showed
R7613	T11770	T11769	compound	panel,A
R7614	T11771	T11769	punct	", ",A
R7615	T11772	T11769	meta	j,A
R7616	T11773	T11772	punct	", ",j
R7617	T11774	T11772	conj	n,j
R7618	T11775	T11774	cc	and,n
R7619	T11776	T11774	conj	r,n
R762	T1549	T1548	prep	for,models
R7620	T11777	T11769	punct	),A
R7621	T11778	T11731	punct	.,showed
R7622	T11780	T11781	prep	At,seen
R7623	T11782	T11783	amod	higher,magnifications
R7624	T11783	T11780	pobj	magnifications,At
R7625	T11784	T11783	prep	of,magnifications
R7626	T11785	T11786	amod	wild,type
R7627	T11786	T11788	compound	type,lungs
R7628	T11787	T11786	punct	-,type
R7629	T11788	T11784	pobj	lungs,of
R763	T1550	T1551	amod	human,SLOS
R7630	T11789	T11790	punct	(,A
R7631	T11790	T11783	parataxis	A,magnifications
R7632	T11791	T11790	compound	panel,A
R7633	T11792	T11790	punct	", ",A
R7634	T11793	T11790	meta	k,A
R7635	T11794	T11793	punct	", ",k
R7636	T11795	T11793	conj	o,k
R7637	T11796	T11795	cc	and,o
R7638	T11797	T11795	conj	s,o
R7639	T11798	T11790	punct	),A
R764	T1551	T1549	pobj	SLOS,for
R7640	T11799	T11781	punct	", ",seen
R7641	T11800	T11801	amod	developing,pre-alveoli
R7642	T11801	T11781	nsubjpass	pre-alveoli,seen
R7643	T11802	T11801	cc	and,pre-alveoli
R7644	T11803	T11804	amod	thinning,mesenchyme
R7645	T11804	T11801	conj	mesenchyme,pre-alveoli
R7646	T11805	T11806	punct	(,arrowheads
R7647	T11806	T11804	parataxis	arrowheads,mesenchyme
R7648	T11807	T11806	punct	),arrowheads
R7649	T11808	T11781	auxpass	were,seen
R765	T1552	T1551	punct	", ",SLOS
R7650	T11809	T11781	cc	but,seen
R7651	T11810	T11811	nsubj	sections,showed
R7652	T11811	T11781	conj	showed,seen
R7653	T11812	T11810	prep	from,sections
R7654	T11813	T11814	compound	knockout,embryos
R7655	T11814	T11812	pobj	embryos,from
R7656	T11815	T11816	det	a,delay
R7657	T11816	T11811	dobj	delay,showed
R7658	T11817	T11811	prep	in,showed
R7659	T11818	T11819	det	this,sequence
R766	T1553	T1554	advmod	especially,patients
R7660	T11819	T11817	pobj	sequence,in
R7661	T11820	T11819	prep	of,sequence
R7662	T11821	T11820	pobj	development,of
R7663	T11822	T11823	punct	(,A
R7664	T11823	T11811	parataxis	A,showed
R7665	T11824	T11823	compound	panel,A
R7666	T11825	T11823	punct	", ",A
R7667	T11826	T11823	meta	l,A
R7668	T11827	T11826	punct	", ",l
R7669	T11828	T11826	conj	p,l
R767	T1554	T1551	appos	patients,SLOS
R7670	T11829	T11828	cc	and,p
R7671	T11830	T11828	conj	t,p
R7672	T11831	T11823	punct	),A
R7673	T11832	T11781	punct	.,seen
R7674	T11834	T11835	prep	At,showed
R7675	T11836	T11834	pobj	birth,At
R7676	T11837	T11835	punct	", ",showed
R7677	T11838	T11835	nsubj	lungs,showed
R7678	T11839	T11838	prep	from,lungs
R7679	T11840	T11841	compound	knockout,pups
R768	T1555	T1554	det	the,patients
R7680	T11841	T11839	pobj	pups,from
R7681	T11842	T11843	amod	deficient,septation
R7682	T11843	T11835	dobj	septation,showed
R7683	T11844	T11843	cc	and,septation
R7684	T11845	T11846	amod	thick,walled
R7685	T11846	T11848	amod	walled,mesenchyme
R7686	T11847	T11846	punct	-,walled
R7687	T11848	T11843	conj	mesenchyme,septation
R7688	T11849	T11850	punct	(,arrowheads
R7689	T11850	T11835	parataxis	arrowheads,showed
R769	T1556	T1557	advmod	more,severely
R7690	T11851	T11852	compound	panel,A
R7691	T11852	T11850	dep	A,arrowheads
R7692	T11853	T11850	punct	", ",arrowheads
R7693	T11854	T11850	meta	x,arrowheads
R7694	T11855	T11850	punct	", ",arrowheads
R7695	T11856	T11850	punct	),arrowheads
R7696	T11857	T11835	punct	.,showed
R7697	T11859	T11860	dep	Labels,de
R7698	T11861	T11860	punct	: ,de
R7699	T11862	T11860	punct	", ",de
R77	T324	T325	amod	apparent,failure
R770	T1557	T1558	advmod	severely,affected
R7700	T11863	T11864	amod	distal,epithelium
R7701	T11864	T11860	appos	epithelium,de
R7702	T11865	T11860	punct	;,de
R7703	T11866	T11867	nmod	m,mesenchyme
R7704	T11867	T11860	appos	mesenchyme,de
R7705	T11868	T11867	punct	", ",mesenchyme
R7706	T11869	T11860	punct	;,de
R7707	T11870	T11860	appos	PA,de
R7708	T11871	T11870	punct	", ",PA
R7709	T11872	T11873	amod	pulmonary,artery
R771	T1558	T1554	amod	affected,patients
R7710	T11873	T11870	appos	artery,PA
R7711	T11874	T11860	punct	;,de
R7712	T11875	T11860	appos	a,de
R7713	T11876	T11875	punct	", ",a
R7714	T11877	T11875	appos	pre-alveoli,a
R7715	T11878	T11860	punct	;,de
R7716	T11879	T11860	appos	b,de
R7717	T11880	T11879	punct	", ",b
R7718	T11881	T11879	appos	bronchi,b
R7719	T11882	T11860	punct	.,de
R772	T1559	T1547	punct	", ",are
R7720	T11884	T11885	compound	Panel,B
R7721	T11885	T11886	nsubj	B,shows
R7722	T11887	T11888	det	the,analyses
R7723	T11888	T11886	dobj	analyses,shows
R7724	T11889	T11888	amod	morphometric,analyses
R7725	T11890	T11888	prep	of,analyses
R7726	T11891	T11892	nmod	lung,sac
R7727	T11892	T11894	compound	sac,spaces
R7728	T11893	T11892	amod	terminal,sac
R7729	T11894	T11890	pobj	spaces,of
R773	T1560	T1561	mark	since,reported
R7730	T11895	T11886	prep	in,shows
R7731	T11896	T11895	pobj	lungs,in
R7732	T11897	T11886	prep	at,shows
R7733	T11898	T11899	amod	various,stages
R7734	T11899	T11897	pobj	stages,at
R7735	T11900	T11899	amod	gestational,stages
R7736	T11901	T11886	punct	.,shows
R7737	T11903	T11904	det	A,reduction
R7738	T11904	T11906	nsubjpass	reduction,noted
R7739	T11905	T11904	amod	significant,reduction
R774	T1561	T1547	advcl	reported,are
R7740	T11907	T11904	prep	in,reduction
R7741	T11908	T11909	det	the,space
R7742	T11909	T11907	pobj	space,in
R7743	T11910	T11909	amod	terminal,space
R7744	T11911	T11909	compound	sac,space
R7745	T11912	T11906	auxpass	was,noted
R7746	T11913	T11906	prep	from,noted
R7747	T11914	T11913	pobj	E17.5,from
R7748	T11915	T11914	advmod	onwards,E17.5
R7749	T11916	T11906	punct	.,noted
R775	T1562	T1563	amod	early,postnatal
R7750	T11918	T11919	nsubjpass	Results,expressed
R7751	T11920	T11919	auxpass	are,expressed
R7752	T11921	T11919	prep	as,expressed
R7753	T11922	T11923	nmod	mean,s.d
R7754	T11923	T11921	pobj	s.d,as
R7755	T11924	T11923	punct	±,s.d
R7756	T11925	T11919	punct	.,expressed
R7757	T12045	T12044	prep	of,Analyses
R7758	T12046	T12047	compound	cell,proliferation
R7759	T12047	T12045	pobj	proliferation,of
R776	T1563	T1564	amod	postnatal,lethality
R7760	T12048	T12047	punct	", ",proliferation
R7761	T12049	T12050	compound	cell,division
R7762	T12050	T12047	conj	division,proliferation
R7763	T12051	T12050	cc	and,division
R7764	T12052	T12053	compound	cell,death
R7765	T12053	T12050	conj	death,division
R7766	T12054	T12044	punct	.,Analyses
R7767	T12056	T12057	compound	Cell,proliferation
R7768	T12057	T12058	nsubjpass	proliferation,analyzed
R7769	T12059	T12060	punct	(,C
R777	T1564	T1561	nsubjpass	lethality,reported
R7770	T12060	T12057	parataxis	C,proliferation
R7771	T12061	T12062	dep	PCNA,A
R7772	T12062	T12060	dep	A,C
R7773	T12063	T12062	punct	: ,A
R7774	T12064	T12062	compound	panels,A
R7775	T12065	T12062	punct	", ",A
R7776	T12066	T12062	conj	B,A
R7777	T12067	T12066	punct	", ",B
R7778	T12068	T12066	conj	E,B
R7779	T12069	T12068	cc	and,E
R778	T1565	T1564	prep	with,lethality
R7780	T12070	T12068	conj	F,E
R7781	T12071	T12060	punct	;,C
R7782	T12072	T12060	dep	BrdU,C
R7783	T12073	T12060	punct	: ,C
R7784	T12074	T12060	compound	panels,C
R7785	T12075	T12060	punct	", ",C
R7786	T12076	T12060	conj	D,C
R7787	T12077	T12076	punct	", ",D
R7788	T12078	T12076	conj	G,D
R7789	T12079	T12078	cc	and,G
R779	T1566	T1567	amod	respiratory,failure
R7790	T12080	T12078	conj	H,G
R7791	T12081	T12060	punct	),C
R7792	T12082	T12057	punct	", ",proliferation
R7793	T12083	T12084	compound	cell,division
R7794	T12084	T12057	conj	division,proliferation
R7795	T12085	T12086	punct	(,K
R7796	T12086	T12084	parataxis	K,division
R7797	T12087	T12086	dep	pHH3,K
R7798	T12088	T12086	punct	: ,K
R7799	T12089	T12086	compound	panels,K
R78	T325	T323	pobj	failure,from
R780	T1567	T1565	pobj	failure,with
R7800	T12090	T12086	punct	", ",K
R7801	T12091	T12086	conj	L,K
R7802	T12092	T12091	punct	", ",L
R7803	T12093	T12091	conj	O,L
R7804	T12094	T12093	cc	and,O
R7805	T12095	T12093	conj	P,O
R7806	T12096	T12086	punct	),K
R7807	T12097	T12084	cc	and,division
R7808	T12098	T12099	compound	cell,death
R7809	T12099	T12084	conj	death,division
R781	T1568	T1561	aux	has,reported
R7810	T12100	T12101	punct	(,M
R7811	T12101	T12099	parataxis	M,death
R7812	T12102	T12101	dep	TUNEL,M
R7813	T12103	T12101	punct	: ,M
R7814	T12104	T12101	compound	panels,M
R7815	T12105	T12101	cc	and,M
R7816	T12106	T12101	conj	N,M
R7817	T12107	T12101	punct	),M
R7818	T12108	T12058	auxpass	were,analyzed
R7819	T12109	T12058	prep	by,analyzed
R782	T1569	T1561	auxpass	been,reported
R7820	T12110	T12109	pobj	immunostaining,by
R7821	T12111	T12110	prep	on,immunostaining
R7822	T12112	T12113	compound	lung,sections
R7823	T12113	T12111	pobj	sections,on
R7824	T12114	T12113	prep	at,sections
R7825	T12115	T12116	amod	early,stages
R7826	T12116	T12114	pobj	stages,at
R7827	T12117	T12115	cc	and,early
R7828	T12118	T12115	conj	late,early
R7829	T12119	T12116	amod	gestational,stages
R783	T1570	T1561	prep	in,reported
R7830	T12120	T12058	punct	.,analyzed
R7831	T12122	T12123	preconj	Both,PCNA
R7832	T12123	T12124	npadvmod	PCNA,labeled
R7833	T12124	T12127	amod	labeled,cells
R7834	T12125	T12123	cc	and,PCNA
R7835	T12126	T12123	conj	BrdU,PCNA
R7836	T12127	T12128	nsubj	cells,were
R7837	T12129	T12127	prep	in,cells
R7838	T12130	T12131	preconj	both,epithelial
R7839	T12131	T12132	amod	epithelial,regions
R784	T1571	T1572	amod	severe,cases
R7840	T12132	T12129	pobj	regions,in
R7841	T12133	T12131	cc	and,epithelial
R7842	T12134	T12131	conj	mesenchymal,epithelial
R7843	T12135	T12128	acomp	comparable,were
R7844	T12136	T12128	prep	at,were
R7845	T12137	T12138	amod	early,stages
R7846	T12138	T12136	pobj	stages,at
R7847	T12139	T12140	punct	(,E14.5
R7848	T12140	T12138	parataxis	E14.5,stages
R7849	T12141	T12140	prep	for,E14.5
R785	T1572	T1570	pobj	cases,in
R7850	T12142	T12141	pobj	PCNA,for
R7851	T12143	T12140	cc	and,E14.5
R7852	T12144	T12140	conj	E13.5,E14.5
R7853	T12145	T12144	prep	for,E13.5
R7854	T12146	T12145	pobj	BrdU,for
R7855	T12147	T12140	punct	),E14.5
R7856	T12148	T12128	prep	between,were
R7857	T12149	T12150	amod	wild,type
R7858	T12150	T12152	nmod	type,lungs
R7859	T12151	T12150	punct	-,type
R786	T1573	T1572	compound	SLOS,cases
R7860	T12152	T12148	pobj	lungs,between
R7861	T12153	T12150	punct	(,type
R7862	T12154	T12155	punct	+,+
R7863	T12155	T12150	appos	+,type
R7864	T12156	T12155	punct	/,+
R7865	T12157	T12150	punct	),type
R7866	T12158	T12150	cc	and,type
R7867	T12159	T12150	conj	knockout,type
R7868	T12160	T12159	punct	(,knockout
R7869	T12161	T12162	punct	-,-
R787	T1574	T1575	punct	[,5
R7870	T12162	T12159	appos	-,knockout
R7871	T12163	T12162	punct	/,-
R7872	T12164	T12152	punct	),lungs
R7873	T12165	T12128	punct	.,were
R7874	T12167	T12168	advmod	Positively,stained
R7875	T12168	T12169	amod	stained,cells
R7876	T12169	T12170	nsubjpass	cells,reduced
R7877	T12171	T12169	prep	in,cells
R7878	T12172	T12173	amod	distal,lungs
R7879	T12173	T12171	pobj	lungs,in
R788	T1575	T1561	parataxis	5,reported
R7880	T12174	T12169	prep	at,cells
R7881	T12175	T12176	amod	late,stage
R7882	T12176	T12174	pobj	stage,at
R7883	T12177	T12178	punct	(,E20.5
R7884	T12178	T12176	parataxis	E20.5,stage
R7885	T12179	T12178	prep	for,E20.5
R7886	T12180	T12179	pobj	PCNA,for
R7887	T12181	T12178	cc	and,E20.5
R7888	T12182	T12178	conj	E18.5,E20.5
R7889	T12183	T12182	prep	for,E18.5
R789	T1576	T1575	nummod	4,5
R7890	T12184	T12183	pobj	BrdU,for
R7891	T12185	T12178	punct	),E20.5
R7892	T12186	T12169	prep	from,cells
R7893	T12187	T12188	compound	knockout,lungs
R7894	T12188	T12186	pobj	lungs,from
R7895	T12189	T12170	auxpass	were,reduced
R7896	T12190	T12170	advmod	markedly,reduced
R7897	T12191	T12170	punct	", ",reduced
R7898	T12192	T12170	prep	compared,reduced
R7899	T12193	T12192	prep	with,compared
R79	T326	T325	amod	respiratory,failure
R790	T1577	T1575	punct	",",5
R7900	T12194	T12195	amod	wild,type
R7901	T12195	T12193	pobj	type,with
R7902	T12196	T12195	punct	-,type
R7903	T12197	T12170	punct	.,reduced
R7904	T12199	T12200	compound	PCNA,labeling
R7905	T12200	T12201	nsubjpass	labeling,attenuated
R7906	T12202	T12200	prep	of,labeling
R7907	T12203	T12204	amod	cardiac,muscle
R7908	T12204	T12202	pobj	muscle,of
R7909	T12205	T12201	auxpass	was,attenuated
R791	T1578	T1575	punct	],5
R7910	T12206	T12201	advmod	also,attenuated
R7911	T12207	T12201	prep	in,attenuated
R7912	T12208	T12209	compound	knockout,embryos
R7913	T12209	T12207	pobj	embryos,in
R7914	T12210	T12201	prep	at,attenuated
R7915	T12211	T12210	pobj	E20.5,at
R7916	T12212	T12213	punct	(,I
R7917	T12213	T12201	parataxis	I,attenuated
R7918	T12214	T12213	advmod	cf.,I
R7919	T12215	T12213	compound	panels,I
R792	T1579	T1547	punct	.,are
R7920	T12216	T12213	cc	and,I
R7921	T12217	T12213	conj	J,I
R7922	T12218	T12213	punct	),I
R7923	T12219	T12201	punct	.,attenuated
R7924	T12221	T12222	det	No,differences
R7925	T12222	T12224	nsubjpass	differences,observed
R7926	T12223	T12222	amod	apparent,differences
R7927	T12225	T12222	prep	in,differences
R7928	T12226	T12227	compound	TUNEL,immunostaining
R7929	T12227	T12225	pobj	immunostaining,in
R793	T1581	T1582	amod	Developmental,abnormalities
R7930	T12228	T12224	auxpass	were,observed
R7931	T12229	T12224	prep	in,observed
R7932	T12230	T12231	compound	lung,sections
R7933	T12231	T12229	pobj	sections,in
R7934	T12232	T12224	prep	at,observed
R7935	T12233	T12232	pobj	E18.5,at
R7936	T12234	T12224	prep	between,observed
R7937	T12235	T12236	amod	wild,type
R7938	T12236	T12234	pobj	type,between
R7939	T12237	T12236	punct	-,type
R794	T1582	T1584	nsubj	abnormalities,are
R7940	T12238	T12236	cc	and,type
R7941	T12239	T12236	conj	knockout,type
R7942	T12240	T12224	punct	.,observed
R7943	T12242	T12243	det	The,immunostaining
R7944	T12243	T12245	nsubj	immunostaining,was
R7945	T12244	T12243	compound	pHH3,immunostaining
R7946	T12246	T12245	acomp	comparable,was
R7947	T12247	T12245	prep	between,was
R7948	T12248	T12249	amod	wild,type
R7949	T12249	T12251	nmod	type,lungs
R795	T1583	T1582	compound	lung,abnormalities
R7950	T12250	T12249	punct	-,type
R7951	T12251	T12247	pobj	lungs,between
R7952	T12252	T12249	cc	and,type
R7953	T12253	T12249	conj	knockout,type
R7954	T12254	T12245	prep	at,was
R7955	T12255	T12254	pobj	E14.5,at
R7956	T12256	T12245	punct	", ",was
R7957	T12257	T12245	cc	and,was
R7958	T12258	T12259	det	the,lungs
R7959	T12259	T12260	nsubj	lungs,showed
R796	T1585	T1584	advmod	also,are
R7960	T12260	T12245	conj	showed,was
R7961	T12261	T12259	prep	from,lungs
R7962	T12262	T12263	amod	wild,type
R7963	T12263	T12261	pobj	type,from
R7964	T12264	T12263	punct	-,type
R7965	T12265	T12259	prep	at,lungs
R7966	T12266	T12265	pobj	E20.5,at
R7967	T12267	T12268	det	a,index
R7968	T12268	T12260	dobj	index,showed
R7969	T12269	T12268	amod	high,index
R797	T1586	T1587	advmod	relatively,common
R7970	T12270	T12268	prep	of,index
R7971	T12271	T12272	amod	positive,cells
R7972	T12272	T12270	pobj	cells,of
R7973	T12273	T12260	punct	", ",showed
R7974	T12274	T12275	mark	whereas,showed
R7975	T12275	T12260	advcl	showed,showed
R7976	T12276	T12275	nsubj	lungs,showed
R7977	T12277	T12276	prep	from,lungs
R7978	T12278	T12277	pobj	knockout,from
R7979	T12279	T12280	det	a,pattern
R798	T1587	T1584	acomp	common,are
R7980	T12280	T12275	dobj	pattern,showed
R7981	T12281	T12280	amod	apparent,pattern
R7982	T12282	T12280	amod	reduced,pattern
R7983	T12283	T12280	compound	staining,pattern
R7984	T12284	T12260	punct	.,showed
R7985	T12287	T12288	det	the,cells
R7986	T12288	T12290	nsubj	cells,are
R7987	T12289	T12288	amod	dividing,cells
R7988	T12290	T12286	advcl	are,Note
R7989	T12291	T12290	advmod	mainly,are
R799	T1588	T1584	prep	in,are
R7990	T12292	T12290	acomp	epithelial,are
R7991	T12293	T12286	punct	.,Note
R7992	T12295	T12296	amod	Original,magnification
R7993	T12296	T12297	dep	magnification,are
R7994	T12298	T12297	punct	: ,are
R7995	T12299	T12300	nsubj	A,are
R7996	T12300	T12297	ccomp	are,are
R7997	T12301	T12302	punct	-,D
R7998	T12302	T12299	prep	D,A
R7999	T12303	T12299	cc	and,A
R8	T246	T247	det	a,model
R80	T327	T325	prep	due,failure
R800	T1589	T1590	compound	SLOS,patients
R8000	T12304	T12299	conj	G,A
R8001	T12305	T12306	punct	-,N
R8002	T12306	T12304	prep	N,G
R8003	T12307	T12300	attr	20X,are
R8004	T12308	T12297	punct	;,are
R8005	T12309	T12297	nsubj	E,are
R8006	T12310	T12309	punct	", ",E
R8007	T12311	T12309	conj	F,E
R8008	T12312	T12311	punct	", ",F
R8009	T12313	T12311	conj	O,F
R801	T1590	T1588	pobj	patients,in
R8010	T12314	T12313	cc	and,O
R8011	T12315	T12313	conj	P,O
R8012	T12316	T12297	attr	40X,are
R8013	T12317	T12297	punct	.,are
R8014	T12360	T12361	compound	Sterol,analyses
R8015	T12362	T12361	prep	of,analyses
R8016	T12363	T12364	amod	embryonic,lungs
R8017	T12364	T12362	pobj	lungs,of
R8018	T12365	T12361	punct	.,analyses
R8019	T12367	T12368	compound	Sterol,contents
R802	T1591	T1584	punct	", ",are
R8020	T12368	T12369	nsubjpass	contents,quantitated
R8021	T12370	T12368	punct	", ",contents
R8022	T12371	T12368	prep	including,contents
R8023	T12372	T12371	pobj	cholesterol,including
R8024	T12373	T12374	punct	(,bars
R8025	T12374	T12372	parataxis	bars,cholesterol
R8026	T12375	T12374	amod	black,bars
R8027	T12376	T12374	punct	),bars
R8028	T12377	T12372	cc	and,cholesterol
R8029	T12378	T12379	nmod	7DHC,8DHC
R803	T1592	T1584	prep	including,are
R8030	T12379	T12372	conj	8DHC,cholesterol
R8031	T12380	T12379	punct	/,8DHC
R8032	T12381	T12382	punct	(,bars
R8033	T12382	T12379	parataxis	bars,8DHC
R8034	T12383	T12382	amod	white,bars
R8035	T12384	T12382	punct	),bars
R8036	T12385	T12369	punct	", ",quantitated
R8037	T12386	T12369	auxpass	were,quantitated
R8038	T12387	T12369	prep	by,quantitated
R8039	T12388	T12389	compound	GC,MS
R804	T1593	T1594	amod	pulmonary,hypoplasia
R8040	T12389	T12387	pobj	MS,by
R8041	T12390	T12389	punct	-,MS
R8042	T12391	T12369	prep	in,quantitated
R8043	T12392	T12393	det	the,lungs
R8044	T12393	T12391	pobj	lungs,in
R8045	T12394	T12393	prep	from,lungs
R8046	T12395	T12396	amod	wild,type
R8047	T12396	T12398	nmod	type,embryos
R8048	T12397	T12396	punct	-,type
R8049	T12398	T12394	pobj	embryos,from
R805	T1594	T1592	pobj	hypoplasia,including
R8050	T12399	T12396	punct	(,type
R8051	T12400	T12401	punct	+,+
R8052	T12401	T12396	appos	+,type
R8053	T12402	T12401	punct	/,+
R8054	T12403	T12396	punct	),type
R8055	T12404	T12396	cc	and,type
R8056	T12405	T12396	conj	knockout,type
R8057	T12406	T12405	punct	(,knockout
R8058	T12407	T12408	punct	-,-
R8059	T12408	T12405	appos	-,knockout
R806	T1595	T1594	punct	", ",hypoplasia
R8060	T12409	T12408	punct	/,-
R8061	T12410	T12405	punct	),knockout
R8062	T12411	T12369	punct	.,quantitated
R8063	T12413	T12414	nsubjpass	Results,expressed
R8064	T12415	T12414	auxpass	are,expressed
R8065	T12416	T12414	prep	as,expressed
R8066	T12417	T12418	nmod	mean,s.d
R8067	T12418	T12416	pobj	s.d,as
R8068	T12419	T12418	punct	±,s.d
R8069	T12420	T12414	cc	and,expressed
R807	T1596	T1597	amod	abnormal,lobation
R8070	T12421	T12422	det	the,number
R8071	T12422	T12423	nsubj	number,is
R8072	T12423	T12414	conj	is,expressed
R8073	T12424	T12422	prep	of,number
R8074	T12425	T12424	pobj	lungs,of
R8075	T12426	T12425	acl	analyzed,lungs
R8076	T12427	T12428	mark	as,indicated
R8077	T12428	T12423	advcl	indicated,is
R8078	T12429	T12423	punct	.,is
R8079	T12431	T12432	amod	Total,sterols
R808	T1597	T1594	appos	lobation,hypoplasia
R8080	T12432	T12433	nsubj	sterols,were
R8081	T12434	T12433	acomp	lower,were
R8082	T12435	T12433	prep	at,were
R8083	T12436	T12437	det	all,stages
R8084	T12437	T12435	pobj	stages,at
R8085	T12438	T12433	prep	in,were
R8086	T12439	T12440	compound	knockout,lungs
R8087	T12440	T12438	pobj	lungs,in
R8088	T12441	T12433	punct	", ",were
R8089	T12442	T12433	prep	with,were
R809	T1598	T1597	amod	pulmonary,lobation
R8090	T12443	T12444	advmod	almost,increase
R8091	T12444	T12442	pobj	increase,with
R8092	T12445	T12444	det	no,increase
R8093	T12446	T12444	prep	in,increase
R8094	T12447	T12448	compound	cholesterol,content
R8095	T12448	T12446	pobj	content,in
R8096	T12449	T12444	prep	with,increase
R8097	T12450	T12449	pobj	time,with
R8098	T12451	T12452	punct	(,bars
R8099	T12452	T12433	parataxis	bars,were
R81	T328	T327	pcomp	to,due
R810	T1599	T1597	cc	and,lobation
R8100	T12453	T12452	amod	black,bars
R8101	T12454	T12452	punct	),bars
R8102	T12455	T12433	punct	", ",were
R8103	T12456	T12433	cc	but,were
R8104	T12457	T12458	expl	there,was
R8105	T12458	T12433	conj	was,were
R8106	T12459	T12460	det	a,increment
R8107	T12460	T12458	attr	increment,was
R8108	T12461	T12460	amod	progressive,increment
R8109	T12462	T12458	prep	in,was
R811	T1600	T1601	amod	pulmonary,arteries
R8110	T12463	T12464	det	the,sterols
R8111	T12464	T12462	pobj	sterols,in
R8112	T12465	T12464	compound	precursor,sterols
R8113	T12466	T12464	punct	", ",sterols
R8114	T12467	T12468	nmod	7DHC,8DHC
R8115	T12468	T12464	appos	8DHC,sterols
R8116	T12469	T12468	punct	/,8DHC
R8117	T12470	T12471	punct	(,bars
R8118	T12471	T12464	parataxis	bars,sterols
R8119	T12472	T12471	amod	open,bars
R812	T1601	T1597	conj	arteries,lobation
R8120	T12473	T12471	punct	),bars
R8121	T12474	T12458	punct	", ",was
R8122	T12475	T12458	prep	with,was
R8123	T12476	T12477	amod	increasing,gestation
R8124	T12477	T12475	pobj	gestation,with
R8125	T12478	T12458	punct	.,was
R8126	T12551	T12552	compound	Surfactant,protein
R8127	T12552	T12553	compound	protein,expression
R8128	T12554	T12553	prep	in,expression
R8129	T12555	T12554	pobj	lungs,in
R813	T1602	T1594	punct	", ",hypoplasia
R8130	T12556	T12553	punct	.,expression
R8131	T12558	T12559	compound	Panel,A
R8132	T12559	T12560	nsubj	A,shows
R8133	T12561	T12562	det	the,patterns
R8134	T12562	T12560	dobj	patterns,shows
R8135	T12563	T12562	compound	immunostaining,patterns
R8136	T12564	T12562	prep	for,patterns
R8137	T12565	T12566	compound	SP,C
R8138	T12566	T12564	pobj	C,for
R8139	T12567	T12566	punct	-,C
R814	T1603	T1594	appos	anomalies,hypoplasia
R8140	T12568	T12562	prep	in,patterns
R8141	T12569	T12568	pobj	lungs,in
R8142	T12570	T12562	prep	at,patterns
R8143	T12571	T12570	pobj	E18.5,at
R8144	T12572	T12571	cc	and,E18.5
R8145	T12573	T12571	conj	E20.5,E18.5
R8146	T12574	T12562	prep	from,patterns
R8147	T12575	T12576	amod	wild,type
R8148	T12576	T12578	nmod	type,embryos
R8149	T12577	T12576	punct	-,type
R815	T1604	T1603	prep	of,anomalies
R8150	T12578	T12574	pobj	embryos,from
R8151	T12579	T12576	punct	(,type
R8152	T12580	T12581	punct	+,+
R8153	T12581	T12576	appos	+,type
R8154	T12582	T12581	punct	/,+
R8155	T12583	T12576	punct	),type
R8156	T12584	T12576	cc	and,type
R8157	T12585	T12576	conj	knockout,type
R8158	T12586	T12585	punct	(,knockout
R8159	T12587	T12588	punct	-,-
R816	T1605	T1606	amod	laryngeal,development
R8160	T12588	T12585	appos	-,knockout
R8161	T12589	T12588	punct	/,-
R8162	T12590	T12585	punct	),knockout
R8163	T12591	T12560	punct	.,shows
R8164	T12593	T12594	det	No,differences
R8165	T12594	T12596	nsubj	differences,were
R8166	T12595	T12594	amod	significant,differences
R8167	T12597	T12594	prep	in,differences
R8168	T12598	T12599	det	the,patterns
R8169	T12599	T12597	pobj	patterns,in
R817	T1606	T1604	pobj	development,of
R8170	T12600	T12599	prep	of,patterns
R8171	T12601	T12602	compound	SP,C
R8172	T12602	T12604	compound	C,staining
R8173	T12603	T12602	punct	-,C
R8174	T12604	T12600	pobj	staining,of
R8175	T12605	T12596	acomp	evident,were
R8176	T12606	T12596	prep	between,were
R8177	T12607	T12608	amod	wild,type
R8178	T12608	T12610	nmod	type,lungs
R8179	T12609	T12608	punct	-,type
R818	T1607	T1605	cc	and,laryngeal
R8180	T12610	T12606	pobj	lungs,between
R8181	T12611	T12608	cc	and,type
R8182	T12612	T12608	conj	knockout,type
R8183	T12613	T12614	punct	(,A
R8184	T12614	T12596	parataxis	A,were
R8185	T12615	T12614	compound	panel,A
R8186	T12616	T12614	punct	", ",A
R8187	T12617	T12614	meta	a,A
R8188	T12618	T12617	punct	-,a
R8189	T12619	T12617	dep	d,a
R819	T1608	T1605	conj	tracheal,laryngeal
R8190	T12620	T12614	punct	),A
R8191	T12621	T12596	punct	.,were
R8192	T12624	T12625	mark	that,expressed
R8193	T12625	T12623	ccomp	expressed,Note
R8194	T12626	T12627	det	the,tubules
R8195	T12627	T12625	nsubj	tubules,expressed
R8196	T12628	T12627	amod	underdeveloped,tubules
R8197	T12629	T12627	amod	epithelial,tubules
R8198	T12630	T12627	prep	in,tubules
R8199	T12631	T12632	compound	knockout,lungs
R82	T329	T330	amod	developmental,abnormalities
R820	T1609	T1610	punct	[,18
R8200	T12632	T12630	pobj	lungs,in
R8201	T12633	T12625	advmod	also,expressed
R8202	T12634	T12635	compound	SP,C
R8203	T12635	T12625	dobj	C,expressed
R8204	T12636	T12635	punct	-,C
R8205	T12637	T12638	punct	(,arrowheads
R8206	T12638	T12625	parataxis	arrowheads,expressed
R8207	T12639	T12640	compound	panel,A
R8208	T12640	T12638	dep	A,arrowheads
R8209	T12641	T12638	punct	", ",arrowheads
R821	T1610	T1584	parataxis	18,are
R8210	T12642	T12638	meta	b,arrowheads
R8211	T12643	T12642	cc	and,b
R8212	T12644	T12642	conj	d,b
R8213	T12645	T12638	punct	", ",arrowheads
R8214	T12646	T12638	punct	),arrowheads
R8215	T12647	T12625	punct	", ",expressed
R8216	T12648	T12625	advcl	indicating,expressed
R8217	T12649	T12650	amod	normal,differentiation
R8218	T12650	T12648	dobj	differentiation,indicating
R8219	T12651	T12650	nmod	type,differentiation
R822	T1611	T1610	punct	],18
R8220	T12652	T12651	nummod	II,type
R8221	T12653	T12650	compound	AECs,differentiation
R8222	T12654	T12623	punct	.,Note
R8223	T12656	T12657	amod	Original,magnification
R8224	T12657	T12658	dep	magnification,10X
R8225	T12659	T12658	punct	: ,10X
R8226	T12660	T12658	punct	.,10X
R8227	T12662	T12663	compound	Panel,B
R8228	T12663	T12664	nsubj	B,shows
R8229	T12665	T12663	punct	", ",B
R823	T1612	T1584	punct	.,are
R8230	T12666	T12667	amod	left,hand
R8231	T12667	T12669	compound	hand,panel
R8232	T12668	T12667	punct	-,hand
R8233	T12669	T12663	appos	panel,B
R8234	T12670	T12663	punct	", ",B
R8235	T12671	T12672	det	a,result
R8236	T12672	T12664	dobj	result,shows
R8237	T12673	T12672	amod	representative,result
R8238	T12674	T12672	prep	from,result
R8239	T12675	T12676	compound	Northern,analyses
R824	T1614	T1615	amod	Mammalian,lung
R8240	T12676	T12674	pobj	analyses,from
R8241	T12677	T12676	prep	of,analyses
R8242	T12678	T12679	amod	total,RNA
R8243	T12679	T12677	pobj	RNA,of
R8244	T12680	T12679	prep	from,RNA
R8245	T12681	T12682	amod	wild,type
R8246	T12682	T12684	nmod	type,lungs
R8247	T12683	T12682	punct	-,type
R8248	T12684	T12680	pobj	lungs,from
R8249	T12685	T12682	punct	", ",type
R825	T1615	T1616	nsubj	lung,is
R8250	T12686	T12682	conj	heterozygous,type
R8251	T12687	T12686	cc	and,heterozygous
R8252	T12688	T12686	conj	knockout,heterozygous
R8253	T12689	T12684	compound	P0,lungs
R8254	T12690	T12676	prep	for,analyses
R8255	T12691	T12692	nmod	SP,A
R8256	T12692	T12694	nmod	A,expression
R8257	T12693	T12692	punct	-,A
R8258	T12694	T12690	pobj	expression,for
R8259	T12695	T12692	punct	", ",A
R826	T1616	T1617	ccomp	is,takes
R8260	T12696	T12697	compound	SP,B
R8261	T12697	T12692	conj	B,A
R8262	T12698	T12697	punct	-,B
R8263	T12699	T12697	punct	", ",B
R8264	T12700	T12701	compound	SP,C
R8265	T12701	T12697	conj	C,B
R8266	T12702	T12701	punct	-,C
R8267	T12703	T12701	cc	and,C
R8268	T12704	T12705	compound	SP,D
R8269	T12705	T12701	conj	D,C
R827	T1618	T1616	acomp	unique,is
R8270	T12706	T12705	punct	-,D
R8271	T12707	T12664	punct	.,shows
R8272	T12709	T12710	compound	Densitometry,analyses
R8273	T12710	T12711	nsubj	analyses,showed
R8274	T12712	T12713	det	the,expression
R8275	T12713	T12715	nsubj	expression,were
R8276	T12714	T12713	amod	relative,expression
R8277	T12715	T12711	advcl	were,showed
R8278	T12716	T12717	punct	(,s.d.
R8279	T12717	T12713	parataxis	s.d.,expression
R828	T1619	T1620	mark	in,is
R8280	T12718	T12719	nsubj	n,3
R8281	T12719	T12717	ccomp	3,s.d.
R8282	T12720	T12719	punct	=,3
R8283	T12721	T12717	punct	", ",s.d.
R8284	T12722	T12717	nmod	mean,s.d.
R8285	T12723	T12717	punct	±,s.d.
R8286	T12724	T12717	punct	),s.d.
R8287	T12725	T12713	prep	of,expression
R8288	T12726	T12727	det	these,mRNAs
R8289	T12727	T12725	pobj	mRNAs,of
R829	T1620	T1616	advcl	is,is
R8290	T12728	T12729	compound	surfactant,protein
R8291	T12729	T12727	compound	protein,mRNAs
R8292	T12730	T12715	neg	not,were
R8293	T12731	T12732	advmod	significantly,different
R8294	T12732	T12715	acomp	different,were
R8295	T12733	T12715	prep	between,were
R8296	T12734	T12735	det	these,genotypes
R8297	T12735	T12733	pobj	genotypes,between
R8298	T12736	T12735	nummod	three,genotypes
R8299	T12737	T12711	punct	.,showed
R83	T330	T327	pobj	abnormalities,due
R830	T1621	T1620	mark	that,is
R8308	T12875	T12874	prep	of,Characterization
R8309	T12876	T12877	nmod	type,AECs
R831	T1622	T1620	nsubj	it,is
R8310	T12877	T12879	compound	AECs,differentiation
R8311	T12878	T12876	nummod	I,type
R8312	T12879	T12875	pobj	differentiation,of
R8313	T12880	T12874	punct	.,Characterization
R8314	T12882	T12883	det	The,saccules
R8315	T12883	T12886	nsubj	saccules,showed
R8316	T12884	T12883	amod	normal,saccules
R8317	T12885	T12883	amod	distal,saccules
R8318	T12887	T12883	prep	of,saccules
R8319	T12888	T12889	amod	wild,type
R832	T1623	T1624	advmod	fully,developed
R8320	T12889	T12887	pobj	type,of
R8321	T12890	T12889	punct	-,type
R8322	T12891	T12889	punct	(,type
R8323	T12892	T12893	punct	+,+
R8324	T12893	T12889	appos	+,type
R8325	T12894	T12893	punct	/,+
R8326	T12895	T12889	punct	),type
R8327	T12896	T12883	prep	at,saccules
R8328	T12897	T12898	amod	near,term
R8329	T12898	T12896	pobj	term,at
R833	T1624	T1620	acomp	developed,is
R8330	T12899	T12898	punct	(,term
R8331	T12900	T12898	appos	E20.5,term
R8332	T12901	T12898	punct	),term
R8333	T12902	T12903	amod	typical,type
R8334	T12903	T12886	dobj	type,showed
R8335	T12904	T12903	nummod	I,type
R8336	T12905	T12903	punct	(,type
R8337	T12906	T12903	appos	t1,type
R8338	T12907	T12903	punct	),type
R8339	T12908	T12903	cc	and,type
R834	T1625	T1616	punct	", ",is
R8340	T12909	T12910	nmod	type,cells
R8341	T12910	T12903	conj	cells,type
R8342	T12911	T12909	nummod	II,type
R8343	T12912	T12909	punct	(,type
R8344	T12913	T12909	appos	t2,type
R8345	T12914	T12909	punct	),type
R8346	T12915	T12910	amod	epithelial,cells
R8347	T12916	T12917	punct	(,arrowheads
R8348	T12917	T12886	parataxis	arrowheads,showed
R8349	T12918	T12919	compound	panel,A
R835	T1626	T1616	cc	but,is
R8350	T12919	T12917	dep	A,arrowheads
R8351	T12920	T12917	punct	", ",arrowheads
R8352	T12921	T12917	punct	),arrowheads
R8353	T12922	T12886	punct	", ",showed
R8354	T12923	T12886	cc	but,showed
R8355	T12924	T12925	nmod	knockout,lungs
R8356	T12925	T12932	nsubj	lungs,showed
R8357	T12926	T12924	punct	(,knockout
R8358	T12927	T12928	punct	-,-
R8359	T12928	T12924	appos	-,knockout
R836	T1627	T1628	aux	does,function
R8360	T12929	T12928	punct	/,-
R8361	T12930	T12925	punct	),lungs
R8362	T12931	T12925	compound	littermate,lungs
R8363	T12932	T12886	conj	showed,showed
R8364	T12933	T12934	advmod	less,developed
R8365	T12934	T12935	amod	developed,saccules
R8366	T12935	T12932	dobj	saccules,showed
R8367	T12936	T12935	acl	lined,saccules
R8368	T12937	T12936	agent	by,lined
R8369	T12938	T12939	amod	columnar,cells
R837	T1628	T1616	conj	function,is
R8370	T12939	T12937	pobj	cells,by
R8371	T12940	T12939	amod	epithelial,cells
R8372	T12941	T12942	punct	(,arrowheads
R8373	T12942	T12932	parataxis	arrowheads,showed
R8374	T12943	T12944	compound	panel,B
R8375	T12944	T12942	dep	B,arrowheads
R8376	T12945	T12942	punct	", ",arrowheads
R8377	T12946	T12942	amod	black,arrowheads
R8378	T12947	T12942	punct	),arrowheads
R8379	T12948	T12932	punct	", ",showed
R838	T1629	T1628	neg	not,function
R8380	T12949	T12932	prep	with,showed
R8381	T12950	T12951	advmod	partially,arrested
R8382	T12951	T12952	amod	arrested,tubules
R8383	T12952	T12949	pobj	tubules,with
R8384	T12953	T12952	prep	in,tubules
R8385	T12954	T12955	det	the,lung
R8386	T12955	T12953	pobj	lung,in
R8387	T12956	T12955	amod	distal,lung
R8388	T12957	T12958	punct	(,arrowheads
R8389	T12958	T12932	parataxis	arrowheads,showed
R839	T1630	T1628	prep	for,function
R8390	T12959	T12960	compound	panel,B
R8391	T12960	T12958	dep	B,arrowheads
R8392	T12961	T12958	punct	", ",arrowheads
R8393	T12962	T12958	amod	green,arrowheads
R8394	T12963	T12958	punct	),arrowheads
R8395	T12964	T12932	punct	.,showed
R8396	T12966	T12967	nsubj	Immunostaining,demonstrated
R8397	T12968	T12966	prep	for,Immunostaining
R8398	T12969	T12970	nmod	type,markers
R8399	T12970	T12968	pobj	markers,for
R84	T331	T330	amod	pulmonary,abnormalities
R840	T1631	T1632	compound	gas,exchange
R8400	T12971	T12969	nummod	I,type
R8401	T12972	T12970	compound	cell,markers
R8402	T12973	T12970	appos	T1α,markers
R8403	T12974	T12973	cc	and,T1α
R8404	T12975	T12973	conj	AQP5,T1α
R8405	T12976	T12977	det	an,staining
R8406	T12977	T12967	dobj	staining,demonstrated
R8407	T12978	T12977	amod	intense,staining
R8408	T12979	T12967	prep	in,demonstrated
R8409	T12980	T12981	amod	flat,cells
R841	T1632	T1630	pobj	exchange,for
R8410	T12981	T12979	pobj	cells,in
R8411	T12982	T12981	amod	epithelial,cells
R8412	T12983	T12967	prep	in,demonstrated
R8413	T12984	T12985	amod	wild,type
R8414	T12985	T12987	compound	type,lungs
R8415	T12986	T12985	punct	-,type
R8416	T12987	T12983	pobj	lungs,in
R8417	T12988	T12967	prep	at,demonstrated
R8418	T12989	T12988	pobj	E20.5,at
R8419	T12990	T12991	punct	(,arrowheads
R842	T1633	T1628	prep	until,function
R8420	T12991	T12967	parataxis	arrowheads,demonstrated
R8421	T12992	T12993	compound	panels,C
R8422	T12993	T12991	dep	C,arrowheads
R8423	T12994	T12993	cc	and,C
R8424	T12995	T12993	conj	E,C
R8425	T12996	T12991	punct	", ",arrowheads
R8426	T12997	T12991	punct	),arrowheads
R8427	T12998	T12967	punct	.,demonstrated
R8428	T13000	T13001	prep	In,decreased
R8429	T13002	T13000	pobj	contrast,In
R843	T1634	T1633	prep	at,until
R8430	T13003	T13001	punct	", ",decreased
R8431	T13004	T13001	prep	in,decreased
R8432	T13005	T13006	compound	knockout,lungs
R8433	T13006	T13004	pobj	lungs,in
R8434	T13007	T13006	compound	littermate,lungs
R8435	T13008	T13001	punct	", ",decreased
R8436	T13009	T13010	nmod	T1α,staining
R8437	T13010	T13001	nsubjpass	staining,decreased
R8438	T13011	T13009	cc	and,T1α
R8439	T13012	T13009	conj	AQP5,T1α
R844	T1635	T1636	det	the,moment
R8440	T13013	T13001	auxpass	was,decreased
R8441	T13014	T13001	advmod	dramatically,decreased
R8442	T13015	T13016	punct	(,arrowheads
R8443	T13016	T13001	parataxis	arrowheads,decreased
R8444	T13017	T13018	compound	panels,D
R8445	T13018	T13016	dep	D,arrowheads
R8446	T13019	T13018	cc	and,D
R8447	T13020	T13018	conj	F,D
R8448	T13021	T13016	punct	", ",arrowheads
R8449	T13022	T13016	punct	),arrowheads
R845	T1636	T1634	pobj	moment,at
R8450	T13023	T13001	punct	.,decreased
R8451	T13025	T13026	compound	Megalin,expression
R8452	T13026	T13027	nsubjpass	expression,detected
R8453	T13028	T13026	punct	(,expression
R8454	T13029	T13026	appos	Meg,expression
R8455	T13030	T13026	punct	),expression
R8456	T13031	T13027	auxpass	was,detected
R8457	T13032	T13027	prep	in,detected
R8458	T13033	T13034	det	the,cells
R8459	T13034	T13032	pobj	cells,in
R846	T1637	T1636	prep	of,moment
R8460	T13035	T13034	nmod	airway,cells
R8461	T13036	T13034	amod	epithelial,cells
R8462	T13037	T13032	punct	", ",in
R8463	T13038	T13032	cc	but,in
R8464	T13039	T13038	neg	not,but
R8465	T13040	T13032	conj	in,in
R8466	T13041	T13042	amod	proximal,cells
R8467	T13042	T13040	pobj	cells,in
R8468	T13043	T13042	nmod	bronchiole,cells
R8469	T13044	T13042	amod	epithelial,cells
R847	T1638	T1637	pobj	birth,of
R8470	T13045	T13046	punct	(,shown
R8471	T13046	T13027	parataxis	shown,detected
R8472	T13047	T13046	nsubj	data,shown
R8473	T13048	T13046	neg	not,shown
R8474	T13049	T13046	punct	),shown
R8475	T13050	T13027	prep	in,detected
R8476	T13051	T13052	det	the,lungs
R8477	T13052	T13050	pobj	lungs,in
R8478	T13053	T13052	prep	from,lungs
R8479	T13054	T13055	preconj	both,type
R848	T1639	T1617	punct	;,takes
R8480	T13055	T13053	pobj	type,from
R8481	T13056	T13055	amod	wild,type
R8482	T13057	T13055	punct	-,type
R8483	T13058	T13055	cc	and,type
R8484	T13059	T13055	conj	knockout,type
R8485	T13060	T13027	prep	at,detected
R8486	T13061	T13060	pobj	E20.5,at
R8487	T13062	T13027	punct	.,detected
R8488	T13064	T13065	det	The,cells
R8489	T13065	T13071	nsubj	cells,were
R849	T1640	T1641	det	the,majority
R8490	T13066	T13065	amod	elongated,cells
R8491	T13067	T13068	npadvmod	megalin,positive
R8492	T13068	T13065	amod	positive,cells
R8493	T13069	T13068	punct	-,positive
R8494	T13070	T13065	amod	epithelial,cells
R8495	T13072	T13065	punct	", ",cells
R8496	T13073	T13065	acl	lining,cells
R8497	T13074	T13075	det	the,sacculi
R8498	T13075	T13073	dobj	sacculi,lining
R8499	T13076	T13077	advmod	normally,developed
R85	T332	T310	punct	.,is
R850	T1641	T1617	nsubj	majority,takes
R8500	T13077	T13075	amod	developed,sacculi
R8501	T13078	T13071	punct	", ",were
R8502	T13079	T13071	advmod	readily,were
R8503	T13080	T13071	acomp	evident,were
R8504	T13081	T13071	prep	in,were
R8505	T13082	T13083	amod	many,areas
R8506	T13083	T13081	pobj	areas,in
R8507	T13084	T13083	prep	of,areas
R8508	T13085	T13086	det	the,lungs
R8509	T13086	T13084	pobj	lungs,of
R851	T1642	T1641	prep	of,majority
R8510	T13087	T13088	amod	wild,type
R8511	T13088	T13086	compound	type,lungs
R8512	T13089	T13088	punct	-,type
R8513	T13090	T13071	punct	.,were
R8514	T13092	T13093	prep	In,comprised
R8515	T13094	T13092	pobj	contrast,In
R8516	T13095	T13093	punct	", ",comprised
R8517	T13096	T13097	det	the,cells
R8518	T13097	T13093	nsubjpass	cells,comprised
R8519	T13098	T13097	amod	terminal,cells
R852	T1643	T1644	amod	structural,development
R8520	T13099	T13097	amod	epithelial,cells
R8521	T13100	T13101	advmod	positively,stained
R8522	T13101	T13097	acl	stained,cells
R8523	T13102	T13101	agent	by,stained
R8524	T13103	T13104	compound	megalin,antibodies
R8525	T13104	T13102	pobj	antibodies,by
R8526	T13105	T13101	prep	in,stained
R8527	T13106	T13107	compound	knockout,lungs
R8528	T13107	T13105	pobj	lungs,in
R8529	T13108	T13093	auxpass	were,comprised
R853	T1644	T1642	pobj	development,of
R8530	T13109	T13093	advmod	mainly,comprised
R8531	T13110	T13093	prep	of,comprised
R8532	T13111	T13112	amod	undifferentiated,cells
R8533	T13112	T13110	pobj	cells,of
R8534	T13113	T13112	amod	cuboidal,cells
R8535	T13114	T13115	punct	(,compare
R8536	T13115	T13093	parataxis	compare,comprised
R8537	T13116	T13117	det	the,arrowheads
R8538	T13117	T13115	dobj	arrowheads,compare
R8539	T13118	T13117	prep	in,arrowheads
R854	T1645	T1644	cc	and,development
R8540	T13119	T13118	pobj	G,in
R8541	T13120	T13119	cc	and,G
R8542	T13121	T13119	conj	H,G
R8543	T13122	T13115	punct	),compare
R8544	T13123	T13093	punct	.,comprised
R8545	T13125	T13126	amod	Original,magnification
R8546	T13126	T13127	dep	magnification,are
R8547	T13128	T13127	punct	: ,are
R8548	T13129	T13130	nsubj	A,are
R8549	T13130	T13127	ccomp	are,are
R855	T1646	T1644	conj	maturation,development
R8550	T13131	T13129	punct	", ",A
R8551	T13132	T13129	conj	B,A
R8552	T13133	T13132	punct	", ",B
R8553	T13134	T13132	conj	G,B
R8554	T13135	T13134	cc	and,G
R8555	T13136	T13134	conj	H,G
R8556	T13137	T13130	attr	60X,are
R8557	T13138	T13127	punct	", ",are
R8558	T13139	T13140	compound	C,F
R8559	T13140	T13127	nsubj	F,are
R856	T1647	T1617	dobj	place,takes
R8560	T13141	T13140	punct	-,F
R8561	T13142	T13127	attr	40X,are
R8562	T13143	T13127	punct	.,are
R8567	T13243	T13244	compound	Immunostaining,patterns
R8568	T13245	T13244	prep	for,patterns
R8569	T13246	T13247	nmod	caveolin,α
R857	T1648	T1649	advmod	in,utero
R8570	T13247	T13245	pobj	α,for
R8571	T13248	T13246	punct	-,caveolin
R8572	T13249	T13246	nummod	1,caveolin
R8573	T13250	T13247	cc	and,α
R8574	T13251	T13247	conj	PECAM,α
R8575	T13252	T13251	punct	-,PECAM
R8576	T13253	T13251	nummod	1,PECAM
R8577	T13254	T13244	prep	in,patterns
R8578	T13255	T13256	compound	lung,sections
R8579	T13256	T13254	pobj	sections,in
R858	T1649	T1617	advmod	utero,takes
R8580	T13257	T13244	punct	.,patterns
R8581	T13259	T13260	compound	Cav,1α
R8582	T13260	T13262	compound	1α,staining
R8583	T13261	T13260	punct	-,1α
R8584	T13262	T13263	nsubjpass	staining,compared
R8585	T13264	T13263	auxpass	was,compared
R8586	T13265	T13263	prep	at,compared
R8587	T13266	T13267	nmod	E14.5,stages
R8588	T13267	T13265	pobj	stages,at
R8589	T13268	T13266	punct	", ",E14.5
R859	T1650	T1617	punct	.,takes
R8590	T13269	T13266	conj	E17.5,E14.5
R8591	T13270	T13269	cc	and,E17.5
R8592	T13271	T13269	conj	E20.5,E17.5
R8593	T13272	T13267	prep	of,stages
R8594	T13273	T13272	pobj	development,of
R8595	T13274	T13263	punct	.,compared
R8596	T13276	T13277	preconj	Both,epithelium
R8597	T13277	T13279	nsubj	epithelium,were
R8598	T13278	T13277	det	the,epithelium
R8599	T13280	T13277	cc	and,epithelium
R86	T336	T337	prep	In,characterized
R860	T1652	T1653	compound	Lung,development
R8600	T13281	T13277	conj	mesenchyme,epithelium
R8601	T13282	T13279	acomp	negative,were
R8602	T13283	T13282	prep	for,negative
R8603	T13284	T13285	compound	cav,1α
R8604	T13285	T13283	pobj	1α,for
R8605	T13286	T13285	punct	-,1α
R8606	T13287	T13279	prep	at,were
R8607	T13288	T13287	pobj	E14.5,at
R8608	T13289	T13279	punct	.,were
R8609	T13291	T13292	amod	Developing,vessels
R861	T1653	T1654	nsubj	development,is
R8610	T13292	T13294	nsubjpass	vessels,stained
R8611	T13293	T13292	compound	blood,vessels
R8612	T13295	T13296	punct	(,arrowheads
R8613	T13296	T13292	parataxis	arrowheads,vessels
R8614	T13297	T13296	amod	red,arrowheads
R8615	T13298	T13296	punct	),arrowheads
R8616	T13299	T13292	cc	and,vessels
R8617	T13300	T13292	conj	capillaries,vessels
R8618	T13301	T13302	punct	(,arrows
R8619	T13302	T13300	parataxis	arrows,capillaries
R862	T1655	T1656	det	a,process
R8620	T13303	T13302	punct	),arrows
R8621	T13304	T13305	punct	(,A
R8622	T13305	T13300	parataxis	A,capillaries
R8623	T13306	T13305	compound	panels,A
R8624	T13307	T13305	cc	and,A
R8625	T13308	T13305	conj	B,A
R8626	T13309	T13305	punct	),A
R8627	T13310	T13294	auxpass	were,stained
R8628	T13311	T13294	advmod	positively,stained
R8629	T13312	T13294	prep	for,stained
R863	T1656	T1654	attr	process,is
R8630	T13313	T13314	compound	cav,1α
R8631	T13314	T13312	pobj	1α,for
R8632	T13315	T13314	punct	-,1α
R8633	T13316	T13294	punct	", ",stained
R8634	T13317	T13294	cc	but,stained
R8635	T13318	T13319	det	the,patterns
R8636	T13319	T13321	nsubjpass	patterns,altered
R8637	T13320	T13319	compound	staining,patterns
R8638	T13321	T13294	conj	altered,stained
R8639	T13322	T13319	prep	between,patterns
R864	T1657	T1656	amod	complex,process
R8640	T13323	T13324	amod	wild,type
R8641	T13324	T13326	nmod	type,lungs
R8642	T13325	T13324	punct	-,type
R8643	T13326	T13322	pobj	lungs,between
R8644	T13327	T13324	punct	(,type
R8645	T13328	T13329	punct	+,+
R8646	T13329	T13324	appos	+,type
R8647	T13330	T13329	punct	/,+
R8648	T13331	T13324	punct	),type
R8649	T13332	T13324	cc	and,type
R865	T1658	T1659	dep	that,involves
R8650	T13333	T13324	conj	knockout,type
R8651	T13334	T13333	punct	(,knockout
R8652	T13335	T13336	punct	-,-
R8653	T13336	T13333	appos	-,knockout
R8654	T13337	T13336	punct	/,-
R8655	T13338	T13333	punct	),knockout
R8656	T13339	T13321	auxpass	were,altered
R8657	T13340	T13321	neg	not,altered
R8658	T13341	T13321	punct	.,altered
R8659	T13343	T13344	nmod	Cav,α
R866	T1659	T1656	relcl	involves,process
R8660	T13344	T13347	nsubjpass	α,detected
R8661	T13345	T13343	punct	-,Cav
R8662	T13346	T13343	nummod	1,Cav
R8663	T13348	T13347	auxpass	was,detected
R8664	T13349	T13347	advmod	also,detected
R8665	T13350	T13347	prep	at,detected
R8666	T13351	T13352	det	the,matrices
R8667	T13352	T13350	pobj	matrices,at
R8668	T13353	T13352	amod	proximal,matrices
R8669	T13354	T13352	nmod	bronchiole,matrices
R867	T1660	T1661	compound	branching,morphogenesis
R8670	T13355	T13352	amod	sub-epithelial,matrices
R8671	T13356	T13357	punct	(,arrowheads
R8672	T13357	T13347	parataxis	arrowheads,detected
R8673	T13358	T13359	compound	panels,A
R8674	T13359	T13357	dep	A,arrowheads
R8675	T13360	T13361	punct	-,D
R8676	T13361	T13359	prep	D,A
R8677	T13362	T13357	punct	", ",arrowheads
R8678	T13363	T13357	amod	black,arrowheads
R8679	T13364	T13357	punct	),arrowheads
R868	T1661	T1659	dobj	morphogenesis,involves
R8680	T13365	T13347	punct	.,detected
R8681	T13367	T13368	prep	At,surrounded
R8682	T13369	T13367	pobj	E17.5,At
R8683	T13370	T13368	punct	", ",surrounded
R8684	T13371	T13372	det	the,epithelium
R8685	T13372	T13368	nsubjpass	epithelium,surrounded
R8686	T13373	T13372	compound	airway,epithelium
R8687	T13374	T13372	punct	", ",epithelium
R8688	T13375	T13372	amod	negative,epithelium
R8689	T13376	T13375	prep	for,negative
R869	T1662	T1661	prep	of,morphogenesis
R8690	T13377	T13378	nmod	cav,α
R8691	T13378	T13381	compound	α,staining
R8692	T13379	T13377	punct	-,cav
R8693	T13380	T13377	nummod	1,cav
R8694	T13381	T13376	pobj	staining,for
R8695	T13382	T13368	punct	", ",surrounded
R8696	T13383	T13368	auxpass	was,surrounded
R8697	T13384	T13368	agent	by,surrounded
R8698	T13385	T13384	pobj	rings,by
R8699	T13386	T13385	prep	of,rings
R87	T338	T339	det	this,study
R870	T1663	T1662	pobj	epithelium,of
R8700	T13387	T13388	nmod	cav,α
R8701	T13388	T13391	nmod	α,networks
R8702	T13389	T13387	punct	-,cav
R8703	T13390	T13387	nummod	1,cav
R8704	T13391	T13386	pobj	networks,of
R8705	T13392	T13393	advmod	positively,stained
R8706	T13393	T13391	amod	stained,networks
R8707	T13394	T13391	amod	capillary,networks
R8708	T13395	T13396	punct	(,arrows
R8709	T13396	T13368	parataxis	arrows,surrounded
R871	T1664	T1661	punct	", ",morphogenesis
R8710	T13397	T13398	compound	panels,C
R8711	T13398	T13396	dep	C,arrows
R8712	T13399	T13398	cc	and,C
R8713	T13400	T13398	conj	D,C
R8714	T13401	T13396	punct	", ",arrows
R8715	T13402	T13396	punct	),arrows
R8716	T13403	T13368	punct	.,surrounded
R8717	T13405	T13406	nmod	Cav,α
R8718	T13406	T13409	compound	α,expression
R8719	T13407	T13405	punct	-,Cav
R872	T1665	T1666	amod	interstitial,development
R8720	T13408	T13405	nummod	1,Cav
R8721	T13409	T13410	compound	expression,patterns
R8722	T13410	T13411	nsubj	patterns,were
R8723	T13412	T13410	prep	in,patterns
R8724	T13413	T13412	pobj	lungs,in
R8725	T13414	T13413	prep	from,lungs
R8726	T13415	T13416	compound	knockout,embryos
R8727	T13416	T13414	pobj	embryos,from
R8728	T13417	T13410	prep	at,patterns
R8729	T13418	T13417	pobj	E17.5,at
R873	T1666	T1661	conj	development,morphogenesis
R8730	T13419	T13411	acomp	similar,were
R8731	T13420	T13419	prep	to,similar
R8732	T13421	T13422	amod	wild,type
R8733	T13422	T13424	compound	type,embryos
R8734	T13423	T13422	punct	-,type
R8735	T13424	T13420	pobj	embryos,to
R8736	T13425	T13411	punct	", ",were
R8737	T13426	T13411	cc	but,were
R8738	T13427	T13428	advmod	relative,less
R8739	T13428	T13429	amod	less,bed
R874	T1667	T1666	prep	including,development
R8740	T13429	T13431	nsubjpass	bed,noted
R8741	T13430	T13429	amod	capillary,bed
R8742	T13431	T13411	conj	noted,were
R8743	T13432	T13431	auxpass	was,noted
R8744	T13433	T13431	prep	in,noted
R8745	T13434	T13435	compound	knockout,lungs
R8746	T13435	T13433	pobj	lungs,in
R8747	T13436	T13437	punct	(,C
R8748	T13437	T13431	parataxis	C,noted
R8749	T13438	T13437	advmod	cf.,C
R875	T1668	T1667	pobj	vasculogenesis,including
R8750	T13439	T13437	compound	panels,C
R8751	T13440	T13437	cc	and,C
R8752	T13441	T13437	conj	D,C
R8753	T13442	T13437	punct	),C
R8754	T13443	T13431	punct	.,noted
R8755	T13445	T13446	prep	At,demonstrated
R8756	T13447	T13445	pobj	E20.5,At
R8757	T13448	T13447	prep	of,E20.5
R8758	T13449	T13450	amod	wild,type
R8759	T13450	T13452	compound	type,lungs
R876	T1669	T1666	punct	", ",development
R8760	T13451	T13450	punct	-,type
R8761	T13452	T13448	pobj	lungs,of
R8762	T13453	T13446	punct	", ",demonstrated
R8763	T13454	T13455	preconj	both,α
R8764	T13455	T13459	nmod	α,staining
R8765	T13456	T13455	nmod	cav,α
R8766	T13457	T13456	punct	-,cav
R8767	T13458	T13456	nummod	1,cav
R8768	T13459	T13446	nsubj	staining,demonstrated
R8769	T13460	T13455	cc	and,α
R877	T1670	T1671	amod	cellular,differentiation
R8770	T13461	T13455	conj	PECAM,α
R8771	T13462	T13461	punct	-,PECAM
R8772	T13463	T13461	nummod	1,PECAM
R8773	T13464	T13465	det	an,network
R8774	T13465	T13446	dobj	network,demonstrated
R8775	T13466	T13465	amod	extensive,network
R8776	T13467	T13465	amod	vascular,network
R8777	T13468	T13446	prep	in,demonstrated
R8778	T13469	T13470	det	the,saccules
R8779	T13470	T13468	pobj	saccules,in
R878	T1671	T1666	conj	differentiation,development
R8780	T13471	T13472	advmod	well,developed
R8781	T13472	T13470	amod	developed,saccules
R8782	T13473	T13446	punct	", ",demonstrated
R8783	T13474	T13475	mark	with,observed
R8784	T13475	T13446	advcl	observed,demonstrated
R8785	T13476	T13475	nsubj	staining,observed
R8786	T13477	T13475	prep	in,observed
R8787	T13478	T13479	amod	close,proximity
R8788	T13479	T13477	pobj	proximity,in
R8789	T13480	T13479	prep	to,proximity
R879	T1672	T1671	punct	", ",differentiation
R8790	T13481	T13482	amod	flat,cells
R8791	T13482	T13480	pobj	cells,to
R8792	T13483	T13482	amod	epithelial,cells
R8793	T13484	T13482	acl	lining,cells
R8794	T13485	T13486	det	the,spaces
R8795	T13486	T13484	dobj	spaces,lining
R8796	T13487	T13486	compound	air,spaces
R8797	T13488	T13489	punct	(,arrows
R8798	T13489	T13446	parataxis	arrows,demonstrated
R8799	T13490	T13491	compound	panels,E
R88	T339	T336	pobj	study,In
R880	T1673	T1674	amod	biochemical,maturation
R8800	T13491	T13489	dep	E,arrows
R8801	T13492	T13491	cc	and,E
R8802	T13493	T13491	conj	G,E
R8803	T13494	T13489	punct	", ",arrows
R8804	T13495	T13489	punct	),arrows
R8805	T13496	T13446	punct	.,demonstrated
R8806	T13498	T13499	advmod	However,observed
R8807	T13500	T13499	punct	", ",observed
R8808	T13501	T13502	amod	delayed,development
R8809	T13502	T13499	nsubjpass	development,observed
R881	T1674	T1671	conj	maturation,differentiation
R8810	T13503	T13502	amod	vascular,development
R8811	T13504	T13499	auxpass	was,observed
R8812	T13505	T13499	prep	in,observed
R8813	T13506	T13507	compound	knockout,lungs
R8814	T13507	T13505	pobj	lungs,in
R8815	T13508	T13499	punct	", ",observed
R8816	T13509	T13499	advcl	showing,observed
R8817	T13510	T13511	nsubjpass	capillaries,embedded
R8818	T13511	T13509	advcl	embedded,showing
R8819	T13512	T13513	punct	(,arrows
R882	T1675	T1674	cc	and,maturation
R8820	T13513	T13510	parataxis	arrows,capillaries
R8821	T13514	T13515	compound	panels,F
R8822	T13515	T13513	dep	F,arrows
R8823	T13516	T13515	cc	and,F
R8824	T13517	T13515	conj	H,F
R8825	T13518	T13513	punct	", ",arrows
R8826	T13519	T13513	punct	),arrows
R8827	T13520	T13510	prep	of,capillaries
R8828	T13521	T13522	det	the,lungs
R8829	T13522	T13520	pobj	lungs,of
R883	T1676	T1677	amod	physical,growth
R8830	T13523	T13522	compound	knockout,lungs
R8831	T13524	T13510	prep	at,capillaries
R8832	T13525	T13524	pobj	E20.5,at
R8833	T13526	T13511	auxpass	were,embedded
R8834	T13527	T13511	prep	in,embedded
R8835	T13528	T13529	det	a,mesenchyme
R8836	T13529	T13527	pobj	mesenchyme,in
R8837	T13530	T13531	advmod	relatively,thick
R8838	T13531	T13529	amod	thick,mesenchyme
R8839	T13532	T13511	prep	around,embedded
R884	T1677	T1674	conj	growth,maturation
R8840	T13533	T13534	det	the,tubules
R8841	T13534	T13532	pobj	tubules,around
R8842	T13535	T13534	amod	undeveloped,tubules
R8843	T13536	T13534	amod	epithelial,tubules
R8844	T13537	T13538	prep	in,remained
R8845	T13538	T13534	relcl	remained,tubules
R8846	T13539	T13537	pobj	which,in
R8847	T13540	T13541	det	the,cells
R8848	T13541	T13538	nsubj	cells,remained
R8849	T13542	T13541	amod	epithelial,cells
R885	T1678	T1654	punct	.,is
R8850	T13543	T13538	dep	were,remained
R8851	T13544	T13538	oprd	cuboidal,remained
R8852	T13545	T13546	punct	(,arrowheads
R8853	T13546	T13511	parataxis	arrowheads,embedded
R8854	T13547	T13546	punct	),arrowheads
R8855	T13548	T13499	punct	.,observed
R8856	T13550	T13551	amod	Original,magnification
R8857	T13551	T13552	dep	magnification,are
R8858	T13553	T13552	punct	: ,are
R8859	T13554	T13555	compound	A,D
R886	T1680	T1681	nummod	Four,stages
R8860	T13555	T13557	nsubj	D,are
R8861	T13556	T13555	punct	-,D
R8862	T13557	T13552	ccomp	are,are
R8863	T13558	T13557	attr	20X,are
R8864	T13559	T13552	punct	;,are
R8865	T13560	T13561	compound	E,H
R8866	T13561	T13552	nsubj	H,are
R8867	T13562	T13561	punct	-,H
R8868	T13563	T13552	attr	40X,are
R8869	T13564	T13552	punct	.,are
R887	T1681	T1682	nsubjpass	stages,described
R8870	T13692	T13693	nmod	Shh,patterns
R8871	T13694	T13692	cc	and,Shh
R8872	T13695	T13692	conj	Ptch,Shh
R8873	T13696	T13693	compound	expression,patterns
R8874	T13697	T13693	prep	at,patterns
R8875	T13698	T13699	amod	different,stages
R8876	T13699	T13697	pobj	stages,at
R8877	T13700	T13699	amod	gestational,stages
R8878	T13701	T13693	prep	in,patterns
R8879	T13702	T13703	nmod	Dhcr7,mice
R888	T1683	T1681	prep	of,stages
R8880	T13703	T13701	pobj	mice,in
R8881	T13704	T13702	punct	-,Dhcr7
R8882	T13705	T13702	punct	/,Dhcr7
R8883	T13706	T13702	punct	-,Dhcr7
R8884	T13707	T13702	cc	and,Dhcr7
R8885	T13708	T13709	amod	wild,type
R8886	T13709	T13702	conj	type,Dhcr7
R8887	T13710	T13709	punct	-,type
R8888	T13711	T13693	punct	.,patterns
R8889	T13713	T13714	amod	Left,panel
R889	T1684	T1685	compound	mouse,development
R8890	T13714	T13715	nsubj	panel,shows
R8891	T13716	T13717	det	the,patterns
R8892	T13717	T13715	dobj	patterns,shows
R8893	T13718	T13719	compound	Shh,immunostaining
R8894	T13719	T13717	compound	immunostaining,patterns
R8895	T13720	T13715	advcl	using,shows
R8896	T13721	T13722	det	an,antibody
R8897	T13722	T13720	dobj	antibody,using
R8898	T13723	T13722	prep	to,antibody
R8899	T13724	T13725	compound	Shh,peptide
R89	T340	T337	punct	", ",characterized
R890	T1685	T1683	pobj	development,of
R8900	T13725	T13723	pobj	peptide,to
R8901	T13726	T13725	compound	N,peptide
R8902	T13727	T13725	punct	-,peptide
R8903	T13728	T13720	prep	at,using
R8904	T13729	T13730	amod	various,stages
R8905	T13730	T13728	pobj	stages,at
R8906	T13731	T13730	amod	gestational,stages
R8907	T13732	T13733	mark	as,indicated
R8908	T13733	T13720	advcl	indicated,using
R8909	T13734	T13733	agent	by,indicated
R891	T1686	T1685	compound	lung,development
R8910	T13735	T13736	det	the,legend
R8911	T13736	T13734	pobj	legend,by
R8912	T13737	T13736	prep	on,legend
R8913	T13738	T13739	det	the,axis
R8914	T13739	T13737	pobj	axis,on
R8915	T13740	T13739	compound	y,axis
R8916	T13741	T13739	punct	-,axis
R8917	T13742	T13715	punct	.,shows
R8918	T13744	T13745	expl	There,were
R8919	T13746	T13747	det	no,differences
R892	T1687	T1682	aux	have,described
R8920	T13747	T13745	attr	differences,were
R8921	T13748	T13747	amod	significant,differences
R8922	T13749	T13747	acl	noted,differences
R8923	T13750	T13749	prep	at,noted
R8924	T13751	T13750	pobj	any,at
R8925	T13752	T13751	prep	of,any
R8926	T13753	T13754	det	the,stages
R8927	T13754	T13752	pobj	stages,of
R8928	T13755	T13751	acl	examined,any
R8929	T13756	T13747	prep	between,differences
R893	T1688	T1682	auxpass	been,described
R8930	T13757	T13758	amod	wild,type
R8931	T13758	T13760	nmod	type,embryos
R8932	T13759	T13758	punct	-,type
R8933	T13760	T13756	pobj	embryos,between
R8934	T13761	T13758	punct	(,type
R8935	T13762	T13763	punct	+,+
R8936	T13763	T13758	appos	+,type
R8937	T13764	T13763	punct	/,+
R8938	T13765	T13758	punct	),type
R8939	T13766	T13758	cc	and,type
R894	T1689	T1682	punct	", ",described
R8940	T13767	T13758	conj	knockout,type
R8941	T13768	T13767	punct	(,knockout
R8942	T13769	T13770	punct	-,-
R8943	T13770	T13767	appos	-,knockout
R8944	T13771	T13770	punct	/,-
R8945	T13772	T13767	punct	),knockout
R8946	T13773	T13745	punct	.,were
R8947	T13775	T13776	prep	At,detected
R8948	T13777	T13775	pobj	E10.5,At
R8949	T13778	T13776	punct	", ",detected
R895	T1690	T1682	prep	based,described
R8950	T13779	T13776	nsubjpass	Shh,detected
R8951	T13780	T13776	auxpass	was,detected
R8952	T13781	T13776	prep	in,detected
R8953	T13782	T13783	det	the,plate
R8954	T13783	T13781	pobj	plate,in
R8955	T13784	T13783	compound	floor,plate
R8956	T13785	T13783	punct	(,plate
R8957	T13786	T13783	appos	fp,plate
R8958	T13787	T13783	punct	),plate
R8959	T13788	T13783	punct	", ",plate
R896	T1691	T1690	prep	upon,based
R8960	T13789	T13783	conj	notochord,plate
R8961	T13790	T13789	punct	(,notochord
R8962	T13791	T13789	appos	nc,notochord
R8963	T13792	T13789	punct	),notochord
R8964	T13793	T13794	punct	(,A
R8965	T13794	T13789	parataxis	A,notochord
R8966	T13795	T13794	cc	and,A
R8967	T13796	T13794	conj	B,A
R8968	T13797	T13794	punct	),A
R8969	T13798	T13789	punct	", ",notochord
R897	T1692	T1693	det	the,appearances
R8970	T13799	T13800	amod	mid,gut
R8971	T13800	T13789	conj	gut,notochord
R8972	T13801	T13800	punct	-,gut
R8973	T13802	T13800	punct	(,gut
R8974	T13803	T13800	appos	mg,gut
R8975	T13804	T13800	punct	),gut
R8976	T13805	T13800	cc	and,gut
R8977	T13806	T13807	det	the,buds
R8978	T13807	T13800	conj	buds,gut
R8979	T13808	T13807	amod	epithelial,buds
R898	T1693	T1691	pobj	appearances,upon
R8980	T13809	T13807	prep	of,buds
R8981	T13810	T13809	pobj	lungs,of
R8982	T13811	T13807	punct	(,buds
R8983	T13812	T13807	appos	lb,buds
R8984	T13813	T13807	punct	),buds
R8985	T13814	T13815	punct	(,C
R8986	T13815	T13807	parataxis	C,buds
R8987	T13816	T13815	cc	and,C
R8988	T13817	T13815	conj	D,C
R8989	T13818	T13815	punct	),C
R899	T1694	T1693	amod	histological,appearances
R8990	T13819	T13776	punct	.,detected
R8991	T13821	T13822	compound	Shh,staining
R8992	T13822	T13823	nsubjpass	staining,restricted
R8993	T13824	T13823	auxpass	was,restricted
R8994	T13825	T13823	prep	to,restricted
R8995	T13826	T13827	amod	epithelial,cells
R8996	T13827	T13825	pobj	cells,to
R8997	T13828	T13823	prep	in,restricted
R8998	T13829	T13830	det	the,region
R8999	T13830	T13828	pobj	region,in
R9	T247	T245	pobj	model,in
R90	T341	T337	nsubj	we,characterized
R900	T1695	T1693	prep	of,appearances
R9000	T13831	T13830	amod	distal,region
R9001	T13832	T13830	prep	of,region
R9002	T13833	T13834	det	the,tubes
R9003	T13834	T13832	pobj	tubes,of
R9004	T13835	T13834	amod	primordial,tubes
R9005	T13836	T13834	prep	of,tubes
R9006	T13837	T13836	pobj	lungs,of
R9007	T13838	T13823	prep	at,restricted
R9008	T13839	T13838	pobj	E13.5,at
R9009	T13840	T13839	cc	and,E13.5
R901	T1696	T1695	pobj	lung,of
R9010	T13841	T13839	conj	E15.5,E13.5
R9011	T13842	T13823	punct	.,restricted
R9012	T13845	T13846	mark	that,immunostained
R9013	T13846	T13844	ccomp	immunostained,Note
R9014	T13847	T13848	amod	proximal,cells
R9015	T13848	T13846	nsubjpass	cells,immunostained
R9016	T13849	T13848	amod	epithelial,cells
R9017	T13850	T13848	prep	of,cells
R9018	T13851	T13852	amod	primordial,tubes
R9019	T13852	T13850	pobj	tubes,of
R902	T1697	T1682	punct	;,described
R9020	T13853	T13846	auxpass	were,immunostained
R9021	T13854	T13846	neg	not,immunostained
R9022	T13855	T13846	prep	for,immunostained
R9023	T13856	T13855	pobj	Shh,for
R9024	T13857	T13858	punct	(,arrows
R9025	T13858	T13846	parataxis	arrows,immunostained
R9026	T13859	T13858	dep	E,arrows
R9027	T13860	T13859	cc	and,E
R9028	T13861	T13859	conj	F,E
R9029	T13862	T13858	punct	", ",arrows
R903	T1698	T1699	amod	pseudoglandular,stages
R9030	T13863	T13858	punct	),arrows
R9031	T13864	T13844	punct	.,Note
R9032	T13866	T13867	nsubjpass	Shh,detected
R9033	T13868	T13867	auxpass	was,detected
R9034	T13869	T13867	prep	in,detected
R9035	T13870	T13871	det	the,cells
R9036	T13871	T13869	pobj	cells,in
R9037	T13872	T13871	nmod	lung,cells
R9038	T13873	T13871	amod	epithelial,cells
R9039	T13874	T13867	prep	on,detected
R904	T1699	T1682	dobj	stages,described
R9040	T13875	T13874	pobj	E18.5,on
R9041	T13876	T13867	punct	", ",detected
R9042	T13877	T13867	cc	and,detected
R9043	T13878	T13867	conj	mainly,detected
R9044	T13879	T13878	prep	in,mainly
R9045	T13880	T13881	det	the,airways
R9046	T13881	T13879	pobj	airways,in
R9047	T13882	T13881	amod	conducting,airways
R9048	T13883	T13878	prep	in,mainly
R9049	T13884	T13885	det	the,neonates
R905	T1700	T1698	punct	", ",pseudoglandular
R9050	T13885	T13883	pobj	neonates,in
R9051	T13886	T13867	punct	.,detected
R9052	T13888	T13889	amod	Original,magnifications
R9053	T13889	T13890	dep	magnifications,are
R9054	T13891	T13890	punct	: ,are
R9055	T13892	T13893	nsubj	A,are
R9056	T13893	T13890	ccomp	are,are
R9057	T13894	T13895	punct	~,H
R9058	T13895	T13892	prep	H,A
R9059	T13896	T13892	punct	", ",A
R906	T1701	T1698	conj	canalicular,pseudoglandular
R9060	T13897	T13892	conj	K,A
R9061	T13898	T13897	cc	and,K
R9062	T13899	T13897	conj	L,K
R9063	T13900	T13893	attr	10X,are
R9064	T13901	T13890	punct	", ",are
R9065	T13902	T13890	nsubj	I,are
R9066	T13903	T13902	cc	and,I
R9067	T13904	T13902	conj	J,I
R9068	T13905	T13890	attr	20X,are
R9069	T13906	T13890	punct	.,are
R907	T1702	T1701	punct	", ",canalicular
R9070	T13908	T13909	amod	Right,panel
R9071	T13909	T13910	nsubj	panel,shows
R9072	T13911	T13912	det	the,patterns
R9073	T13912	T13910	dobj	patterns,shows
R9074	T13913	T13912	prep	of,patterns
R9075	T13914	T13915	compound	Ptch,IHC
R9076	T13915	T13913	pobj	IHC,of
R9077	T13916	T13912	prep	at,patterns
R9078	T13917	T13918	amod	various,stages
R9079	T13918	T13916	pobj	stages,at
R908	T1703	T1704	amod	terminal,saccular
R9080	T13919	T13918	amod	gestational,stages
R9081	T13920	T13910	punct	.,shows
R9082	T13922	T13923	nsubj	Immunostaining,confirmed
R9083	T13924	T13922	prep	of,Immunostaining
R9084	T13925	T13924	pobj	Ptch,of
R9085	T13926	T13922	prep	from,Immunostaining
R9086	T13927	T13926	pobj	E13.5,from
R9087	T13928	T13926	prep	to,from
R9088	T13929	T13928	pobj	P,to
R9089	T13930	T13929	nummod	0,P
R909	T1704	T1701	conj	saccular,canalicular
R9090	T13931	T13923	dobj	concordance,confirmed
R9091	T13932	T13931	prep	with,concordance
R9092	T13933	T13934	compound	Shh,expression
R9093	T13934	T13932	pobj	expression,with
R9094	T13935	T13923	cc	and,confirmed
R9095	T13936	T13923	conj	was,confirmed
R9096	T13937	T13936	neg	not,was
R9097	T13938	T13939	advmod	significantly,different
R9098	T13939	T13936	acomp	different,was
R9099	T13940	T13936	prep	between,was
R91	T342	T337	advmod	further,characterized
R910	T1705	T1704	cc	and,saccular
R9100	T13941	T13942	amod	wild,type
R9101	T13942	T13944	nmod	type,embryos
R9102	T13943	T13942	punct	-,type
R9103	T13944	T13940	pobj	embryos,between
R9104	T13945	T13942	cc	and,type
R9105	T13946	T13942	conj	knockout,type
R9106	T13947	T13923	punct	.,confirmed
R9107	T13950	T13951	mark	that,immunostained
R9108	T13951	T13949	ccomp	immunostained,Note
R9109	T13952	T13953	det	the,cells
R911	T1706	T1704	conj	alveolar,saccular
R9110	T13953	T13951	nsubjpass	cells,immunostained
R9111	T13954	T13953	amod	proximal,cells
R9112	T13955	T13953	amod	epithelial,cells
R9113	T13956	T13953	prep	of,cells
R9114	T13957	T13958	amod	primordial,tubes
R9115	T13958	T13956	pobj	tubes,of
R9116	T13959	T13951	auxpass	were,immunostained
R9117	T13960	T13951	advmod	also,immunostained
R9118	T13961	T13951	neg	not,immunostained
R9119	T13962	T13951	prep	for,immunostained
R912	T1707	T1708	punct	[,19
R9120	T13963	T13962	pobj	Ptch,for
R9121	T13964	T13951	prep	at,immunostained
R9122	T13965	T13966	compound	E13.5,lungs
R9123	T13966	T13964	pobj	lungs,at
R9124	T13967	T13968	punct	(,arrows
R9125	T13968	T13951	parataxis	arrows,immunostained
R9126	T13969	T13968	punct	),arrows
R9127	T13970	T13949	punct	.,Note
R9128	T13972	T13973	amod	Original,magnifications
R9129	T13973	T13974	nsubj	magnifications,are
R913	T1708	T1682	parataxis	19,described
R9130	T13975	T13973	punct	: ,magnifications
R9131	T13976	T13973	appos	A,magnifications
R9132	T13977	T13978	punct	~,F
R9133	T13978	T13976	prep	F,A
R9134	T13979	T13976	punct	", ",A
R9135	T13980	T13976	appos	I,A
R9136	T13981	T13980	cc	and,I
R9137	T13982	T13980	conj	J,I
R9138	T13983	T13974	attr	10X,are
R9139	T13984	T13974	punct	.,are
R914	T1709	T1708	punct	],19
R9140	T13986	T13987	nsubj	G,are
R9141	T13988	T13986	cc	and,G
R9142	T13989	T13986	conj	H,G
R9143	T13990	T13987	attr	20X,are
R9144	T13991	T13987	punct	.,are
R9145	T14031	T14030	prep	of,Comparisons
R9146	T14032	T14033	nmod	Smo,expression
R9147	T14033	T14031	pobj	expression,of
R9148	T14034	T14032	punct	", ",Smo
R9149	T14035	T14032	conj	Gli1,Smo
R915	T1710	T1682	punct	.,described
R9150	T14036	T14035	cc	and,Gli1
R9151	T14037	T14035	conj	Gli3,Gli1
R9152	T14038	T14030	prep	in,Comparisons
R9153	T14039	T14040	compound	lung,sections
R9154	T14040	T14038	pobj	sections,in
R9155	T14041	T14030	punct	.,Comparisons
R9156	T14043	T14044	nsubj	Smoothened,showed
R9157	T14045	T14043	punct	(,Smoothened
R9158	T14046	T14043	appos	Smo,Smoothened
R9159	T14047	T14048	punct	", ",panel
R916	T1712	T1713	amod	Impaired,function
R9160	T14048	T14043	parataxis	panel,Smoothened
R9161	T14049	T14048	amod	upper,panel
R9162	T14050	T14048	punct	),panel
R9163	T14051	T14043	punct	", ",Smoothened
R9164	T14052	T14043	conj	Gli1,Smoothened
R9165	T14053	T14054	punct	(,panel
R9166	T14054	T14052	parataxis	panel,Gli1
R9167	T14055	T14054	amod	middle,panel
R9168	T14056	T14054	punct	),panel
R9169	T14057	T14052	cc	and,Gli1
R917	T1713	T1715	nsubjpass	function,proposed
R9170	T14058	T14052	conj	Gli3,Gli1
R9171	T14059	T14060	punct	(,panel
R9172	T14060	T14058	parataxis	panel,Gli3
R9173	T14061	T14060	compound	bottom,panel
R9174	T14062	T14060	punct	),panel
R9175	T14063	T14064	det	a,pattern
R9176	T14064	T14044	dobj	pattern,showed
R9177	T14065	T14064	prep	of,pattern
R9178	T14066	T14065	pobj	distribution,of
R9179	T14067	T14064	amod	similar,pattern
R918	T1714	T1713	compound	hedgehog,function
R9180	T14068	T14067	prep	to,similar
R9181	T14069	T14068	pobj	that,to
R9182	T14070	T14069	acl	seen,that
R9183	T14071	T14070	prep	with,seen
R9184	T14072	T14071	pobj	Shh,with
R9185	T14073	T14072	cc	and,Shh
R9186	T14074	T14072	conj	Ptch,Shh
R9187	T14075	T14076	punct	(,compare
R9188	T14076	T14044	parataxis	compare,showed
R9189	T14077	T14076	prep	to,compare
R919	T1716	T1715	aux	has,proposed
R9190	T14078	T14077	pobj	Fig.,to
R9191	T14079	T14078	nummod	7,Fig.
R9192	T14080	T14076	punct	),compare
R9193	T14081	T14044	punct	.,showed
R9194	T14083	T14084	det	No,differences
R9195	T14084	T14085	nsubj	differences,were
R9196	T14086	T14084	prep	between,differences
R9197	T14087	T14088	amod	wild,type
R9198	T14088	T14090	nmod	type,embryos
R9199	T14089	T14088	punct	-,type
R92	T343	T344	det	the,role
R920	T1717	T1715	auxpass	been,proposed
R9200	T14090	T14086	pobj	embryos,between
R9201	T14091	T14088	punct	(,type
R9202	T14092	T14093	punct	+,+
R9203	T14093	T14088	appos	+,type
R9204	T14094	T14093	punct	/,+
R9205	T14095	T14088	punct	),type
R9206	T14096	T14088	cc	and,type
R9207	T14097	T14088	conj	knockout,type
R9208	T14098	T14097	punct	(,knockout
R9209	T14099	T14100	punct	-,-
R921	T1718	T1715	prep	as,proposed
R9210	T14100	T14097	appos	-,knockout
R9211	T14101	T14100	punct	/,-
R9212	T14102	T14090	punct	),embryos
R9213	T14103	T14085	acomp	evident,were
R9214	T14104	T14085	punct	.,were
R9215	T14106	T14107	amod	Original,magnifications
R9216	T14107	T14108	dep	magnifications,20X
R9217	T14109	T14108	punct	: ,20X
R9218	T14110	T14108	punct	.,20X
R9219	T14213	T14214	compound	Shh,protein
R922	T1719	T1720	det	a,mechanism
R9220	T14214	T14215	compound	protein,autoprocessing
R9221	T14216	T14215	prep	in,autoprocessing
R9222	T14217	T14218	compound	mouse,embryos
R9223	T14218	T14216	pobj	embryos,in
R9224	T14219	T14215	punct	.,autoprocessing
R9225	T14221	T14222	compound	Western,blotting
R9226	T14222	T14223	nsubjpass	blotting,probed
R9227	T14224	T14222	prep	of,blotting
R9228	T14225	T14224	pobj	lysates,of
R9229	T14226	T14225	prep	from,lysates
R923	T1720	T1718	pobj	mechanism,as
R9230	T14227	T14228	amod	wild,type
R9231	T14228	T14230	nmod	type,embryos
R9232	T14229	T14228	punct	-,type
R9233	T14230	T14226	pobj	embryos,from
R9234	T14231	T14232	punct	(,track
R9235	T14232	T14228	parataxis	track,type
R9236	T14233	T14232	nummod	3,track
R9237	T14234	T14232	punct	),track
R9238	T14235	T14228	punct	", ",type
R9239	T14236	T14228	conj	heterozygous,type
R924	T1721	T1720	acl	underlying,mechanism
R9240	T14237	T14238	punct	(,track
R9241	T14238	T14236	parataxis	track,heterozygous
R9242	T14239	T14238	nummod	4,track
R9243	T14240	T14238	punct	),track
R9244	T14241	T14236	cc	and,heterozygous
R9245	T14242	T14236	conj	knockout,heterozygous
R9246	T14243	T14244	punct	(,track
R9247	T14244	T14242	parataxis	track,knockout
R9248	T14245	T14244	nummod	5,track
R9249	T14246	T14244	punct	),track
R925	T1722	T1721	dobj	malformations,underlying
R9250	T14247	T14230	prep	at,embryos
R9251	T14248	T14247	pobj	E11.5,at
R9252	T14249	T14230	punct	", ",embryos
R9253	T14250	T14230	cc	or,embryos
R9254	T14251	T14252	compound	COS1,cells
R9255	T14252	T14230	conj	cells,embryos
R9256	T14253	T14252	acl	transfected,cells
R9257	T14254	T14253	prep	with,transfected
R9258	T14255	T14254	pobj	Shh,with
R9259	T14256	T14257	punct	(,track
R926	T1723	T1722	acl	found,malformations
R9260	T14257	T14255	parataxis	track,Shh
R9261	T14258	T14257	nummod	1,track
R9262	T14259	T14257	punct	),track
R9263	T14260	T14255	cc	or,Shh
R9264	T14261	T14262	compound	Shh,N
R9265	T14262	T14255	conj	N,Shh
R9266	T14263	T14262	punct	-,N
R9267	T14264	T14265	punct	(,track
R9268	T14265	T14262	parataxis	track,N
R9269	T14266	T14265	nummod	2,track
R927	T1724	T1723	prep	in,found
R9270	T14267	T14265	punct	),track
R9271	T14268	T14223	auxpass	were,probed
R9272	T14269	T14223	prep	with,probed
R9273	T14270	T14271	compound	anti-Shh,N19
R9274	T14271	T14273	compound	N19,antibodies
R9275	T14272	T14271	punct	-,N19
R9276	T14273	T14269	pobj	antibodies,with
R9277	T14274	T14223	punct	.,probed
R9278	T14276	T14277	nummod	20,g
R9279	T14277	T14279	nsubjpass	g,loaded
R928	T1725	T1724	pobj	SLOS,in
R9280	T14278	T14276	quantmod	μ,20
R9281	T14280	T14277	prep	of,g
R9282	T14281	T14282	compound	lysate,proteins
R9283	T14282	T14280	pobj	proteins,of
R9284	T14283	T14282	prep	from,proteins
R9285	T14284	T14285	npadvmod	Shh,transfected
R9286	T14285	T14286	amod	transfected,cells
R9287	T14286	T14283	pobj	cells,from
R9288	T14287	T14286	compound	COS1,cells
R9289	T14288	T14277	cc	and,g
R929	T1726	T1715	punct	.,proposed
R9290	T14289	T14290	nummod	75,g
R9291	T14290	T14277	conj	g,g
R9292	T14291	T14289	quantmod	μ,75
R9293	T14292	T14290	prep	from,g
R9294	T14293	T14292	pobj	embryos,from
R9295	T14294	T14279	auxpass	was,loaded
R9296	T14295	T14279	punct	.,loaded
R9297	T14297	T14298	preconj	Both,unprocessed
R9298	T14298	T14299	amod	unprocessed,forms
R9299	T14299	T14328	nsubjpass	forms,detected
R93	T344	T337	dobj	role,characterized
R930	T1728	T1729	compound	Hedgehog,members
R9300	T14300	T14301	punct	(,signal
R9301	T14301	T14298	parataxis	signal,unprocessed
R9302	T14302	T14301	dep	Shh,signal
R9303	T14303	T14301	punct	", ",signal
R9304	T14304	T14305	amod	upper,arrow
R9305	T14305	T14301	dep	arrow,signal
R9306	T14306	T14307	punct	~,45
R9307	T14307	T14308	nummod	45,kDa
R9308	T14308	T14301	dep	kDa,signal
R9309	T14309	T14301	punct	", ",signal
R931	T1729	T1731	nsubj	members,play
R9310	T14310	T14301	amod	faint,signal
R9311	T14311	T14301	punct	),signal
R9312	T14312	T14298	cc	and,unprocessed
R9313	T14313	T14298	conj	processed,unprocessed
R9314	T14314	T14315	punct	(,track
R9315	T14315	T14313	parataxis	track,processed
R9316	T14316	T14317	compound	Shh,Np
R9317	T14317	T14315	dep	Np,track
R9318	T14318	T14317	punct	-,Np
R9319	T14319	T14315	punct	", ",track
R932	T1730	T1729	compound	family,members
R9320	T14320	T14321	amod	lowest,arrow
R9321	T14321	T14315	dep	arrow,track
R9322	T14322	T14315	punct	", ",track
R9323	T14323	T14324	amod	strong,signal
R9324	T14324	T14315	dep	signal,track
R9325	T14325	T14315	punct	", ",track
R9326	T14326	T14315	nummod	1,track
R9327	T14327	T14315	punct	),track
R9328	T14329	T14299	prep	of,forms
R9329	T14330	T14329	pobj	Shh,of
R933	T1732	T1733	amod	diverse,roles
R9330	T14331	T14328	auxpass	were,detected
R9331	T14332	T14328	prep	from,detected
R9332	T14333	T14334	npadvmod	Shh,transfected
R9333	T14334	T14335	amod	transfected,cells
R9334	T14335	T14332	pobj	cells,from
R9335	T14336	T14335	compound	COS1,cells
R9336	T14337	T14328	punct	.,detected
R9337	T14339	T14340	det	The,bands
R9338	T14340	T14344	nsubj	bands,represented
R9339	T14341	T14340	nummod	two,bands
R934	T1733	T1731	dobj	roles,play
R9340	T14342	T14340	amod	slower,bands
R9341	T14343	T14340	amod	migrating,bands
R9342	T14345	T14340	prep	in,bands
R9343	T14346	T14347	compound	Shh,N
R9344	T14347	T14349	npadvmod	N,transfected
R9345	T14348	T14347	punct	-,N
R9346	T14349	T14350	amod	transfected,cells
R9347	T14350	T14345	pobj	cells,in
R9348	T14351	T14350	compound	COS1,cells
R9349	T14352	T14353	punct	(,track
R935	T1734	T1731	prep	in,play
R9350	T14353	T14340	parataxis	track,bands
R9351	T14354	T14353	nummod	2,track
R9352	T14355	T14353	punct	),track
R9353	T14356	T14357	compound	Shh,N
R9354	T14357	T14359	compound	N,proteins
R9355	T14358	T14357	punct	-,N
R9356	T14359	T14344	dobj	proteins,represented
R9357	T14360	T14344	prep	with,represented
R9358	T14361	T14362	amod	different,modifications
R9359	T14362	T14360	pobj	modifications,with
R936	T1735	T1736	amod	embryonic,development
R9360	T14363	T14362	compound	lipid,modifications
R9361	T14364	T14362	prep	at,modifications
R9362	T14365	T14366	poss	its,terminus
R9363	T14366	T14364	pobj	terminus,at
R9364	T14367	T14366	compound	N,terminus
R9365	T14368	T14344	punct	.,represented
R9366	T14370	T14371	det	A,band
R9367	T14371	T14373	nsubjpass	band,detected
R9368	T14372	T14371	amod	major,band
R9369	T14374	T14371	acl	running,band
R937	T1736	T1734	pobj	development,in
R9370	T14375	T14374	prep	at,running
R9371	T14376	T14377	det	the,position
R9372	T14377	T14375	pobj	position,at
R9373	T14378	T14377	amod	same,position
R9374	T14379	T14377	prep	as,position
R9375	T14380	T14381	amod	processed,Shh
R9376	T14381	T14379	pobj	Shh,as
R9377	T14382	T14373	auxpass	was,detected
R9378	T14383	T14373	prep	in,detected
R9379	T14384	T14383	pobj	lysates,in
R938	T1737	T1731	punct	", ",play
R9380	T14385	T14384	prep	from,lysates
R9381	T14386	T14387	predet	all,embryos
R9382	T14387	T14385	pobj	embryos,from
R9383	T14388	T14387	det	the,embryos
R9384	T14389	T14390	punct	(,3
R9385	T14390	T14373	parataxis	3,detected
R9386	T14391	T14390	nmod	tracks,3
R9387	T14392	T14393	punct	–,5
R9388	T14393	T14390	prep	5,3
R9389	T14394	T14390	punct	),3
R939	T1738	T1731	cc	and,play
R9390	T14395	T14373	punct	.,detected
R9391	T14397	T14398	advmod	Importantly,detected
R9392	T14399	T14398	punct	", ",detected
R9393	T14400	T14401	det	no,amounts
R9394	T14401	T14398	nsubjpass	amounts,detected
R9395	T14402	T14401	amod	significant,amounts
R9396	T14403	T14401	prep	of,amounts
R9397	T14404	T14405	amod	unprocessed,proteins
R9398	T14405	T14403	pobj	proteins,of
R9399	T14406	T14404	cc	or,unprocessed
R94	T345	T344	prep	of,role
R940	T1739	T1740	nsubj	cholesterol,is
R9400	T14407	T14408	advmod	aberrantly,migrating
R9401	T14408	T14404	conj	migrating,unprocessed
R9402	T14409	T14405	compound	Shh,proteins
R9403	T14410	T14398	auxpass	were,detected
R9404	T14411	T14398	prep	in,detected
R9405	T14412	T14411	pobj	lysates,in
R9406	T14413	T14412	prep	from,lysates
R9407	T14414	T14415	compound	knockout,embryos
R9408	T14415	T14413	pobj	embryos,from
R9409	T14416	T14398	prep	at,detected
R941	T1740	T1731	conj	is,play
R9410	T14417	T14418	det	this,stage
R9411	T14418	T14416	pobj	stage,at
R9412	T14419	T14398	punct	.,detected
R9413	T14428	T14429	amod	Whole,body
R9414	T14429	T14430	nmod	body,weights
R9415	T14431	T14429	cc	and,body
R9416	T14432	T14429	conj	lung,body
R9417	T14433	T14430	prep	of,weights
R9418	T14434	T14435	compound	mouse,embryos
R9419	T14435	T14433	pobj	embryos,of
R942	T1741	T1740	acomp	necessary,is
R943	T1742	T1741	prep	for,necessary
R944	T1743	T1742	pobj	maturation,for
R945	T1744	T1743	prep	of,maturation
R946	T1745	T1746	det	these,morphogens
R947	T1746	T1744	pobj	morphogens,of
R948	T1747	T1748	punct	[,20
R949	T1748	T1740	parataxis	20,is
R95	T346	T347	compound	cholesterol,deficiency
R950	T1749	T1750	punct	-,23
R951	T1750	T1748	prep	23,20
R952	T1751	T1750	punct	],23
R953	T1752	T1740	punct	.,is
R954	T1754	T1755	det	The,cascade
R955	T1755	T1762	nsubjpass	cascade,shown
R956	T1756	T1757	amod	sonic,hedgehog
R957	T1757	T1755	nmod	hedgehog,cascade
R958	T1758	T1757	punct	(,hedgehog
R959	T1759	T1757	appos	Shh,hedgehog
R96	T347	T345	pobj	deficiency,of
R960	T1760	T1755	punct	),cascade
R961	T1761	T1755	compound	signaling,cascade
R962	T1763	T1762	aux	has,shown
R963	T1764	T1762	auxpass	been,shown
R964	T1765	T1766	aux	to,play
R965	T1766	T1762	xcomp	play,shown
R966	T1767	T1768	det	a,role
R967	T1768	T1766	dobj	role,play
R968	T1769	T1768	amod	central,role
R969	T1770	T1766	prep	in,play
R97	T348	T344	prep	in,role
R970	T1771	T1772	compound	lung,development
R971	T1772	T1770	pobj	development,in
R972	T1773	T1774	punct	[,24
R973	T1774	T1766	parataxis	24,play
R974	T1775	T1776	punct	-,26
R975	T1776	T1774	prep	26,24
R976	T1777	T1774	punct	],24
R977	T1778	T1762	punct	.,shown
R978	T1780	T1781	nsubj	Loss,resulted
R979	T1782	T1780	prep	of,Loss
R98	T349	T350	compound	lung,development
R980	T1783	T1784	compound	Shh,function
R981	T1784	T1782	pobj	function,of
R982	T1785	T1781	prep	in,resulted
R983	T1786	T1787	amod	severe,defects
R984	T1787	T1785	pobj	defects,in
R985	T1788	T1787	compound	lung,defects
R986	T1789	T1787	acl	associated,defects
R987	T1790	T1789	prep	with,associated
R988	T1791	T1790	pobj	loss,with
R989	T1792	T1791	prep	of,loss
R99	T350	T348	pobj	development,in
R990	T1793	T1794	amod	branching,morphogenesis
R991	T1794	T1792	pobj	morphogenesis,of
R992	T1795	T1781	punct	", ",resulted
R993	T1796	T1781	cc	but,resulted
R994	T1797	T1781	conj	preserved,resulted
R995	T1798	T1799	amod	proximal,differentiation
R996	T1799	T1797	dobj	differentiation,preserved
R997	T1800	T1798	punct	-,proximal
R998	T1801	T1798	prep	to,proximal
R999	T1802	T1801	punct	-,to

PMC:341451 JSONTXT 8 Projects

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PMC:341451 JSON TXT 8 Projects