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PMC:340383 / 2336-3691 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T863 0-9 JJ denotes Molecular
T864 10-17 NN denotes cloning
T865 18-20 IN denotes of
T866 21-24 DT denotes the
T868 25-29 NN denotes Acdp
T869 30-34 NN denotes gene
T867 35-41 NN denotes family
T870 41-205 sentence denotes To clone the mouse Acdp genes, the human ACDP cDNA and predicted protein sequences were used to search the mouse EST database with the blastn and tblastn programs.
T871 42-44 TO denotes To
T872 45-50 VB denotes clone
T874 51-54 DT denotes the
T876 55-60 NN denotes mouse
T877 61-65 NN denotes Acdp
T875 66-71 NNS denotes genes
T878 71-73 , denotes ,
T879 73-76 DT denotes the
T881 77-82 JJ denotes human
T882 83-87 NN denotes ACDP
T880 88-92 NN denotes cDNA
T883 93-96 CC denotes and
T884 97-106 JJ denotes predicted
T886 107-114 NN denotes protein
T885 115-124 NNS denotes sequences
T887 125-129 VBD denotes were
T873 130-134 VBN denotes used
T888 135-137 TO denotes to
T889 138-144 VB denotes search
T890 145-148 DT denotes the
T892 149-154 NN denotes mouse
T893 155-158 NN denotes EST
T891 159-167 NN denotes database
T894 168-172 IN denotes with
T895 173-176 DT denotes the
T897 177-183 NN denotes blastn
T898 184-187 CC denotes and
T899 188-195 NN denotes tblastn
T896 196-204 NNS denotes programs
T900 204-205 . denotes .
T901 205-279 sentence denotes Mouse EST markers corresponding to each Acdp member were then identified.
T902 206-211 NN denotes Mouse
T904 212-215 NN denotes EST
T903 216-223 NNS denotes markers
T906 224-237 VBG denotes corresponding
T907 238-240 IN denotes to
T908 241-245 DT denotes each
T910 246-250 NN denotes Acdp
T909 251-257 NN denotes member
T911 258-262 VBD denotes were
T912 263-267 RB denotes then
T905 268-278 VBN denotes identified
T913 278-279 . denotes .
T914 279-393 sentence denotes For example, EST H3086H12-5 corresponds to Acdp1, W98010 for Acdp2, 603299135F1 for Acdp3 and BG083791 for Acdp4.
T915 280-283 IN denotes For
T917 284-291 NN denotes example
T918 291-293 , denotes ,
T919 293-296 NN denotes EST
T920 297-305 NN denotes H3086H12
T921 305-306 HYPH denotes -
T922 306-307 CD denotes 5
T916 308-319 VBZ denotes corresponds
T923 320-322 IN denotes to
T924 323-328 NN denotes Acdp1
T925 328-330 , denotes ,
T926 330-336 NN denotes W98010
T927 337-340 IN denotes for
T928 341-346 NN denotes Acdp2
T929 346-348 , denotes ,
T930 348-359 NN denotes 603299135F1
T931 360-363 IN denotes for
T932 364-369 NN denotes Acdp3
T933 370-373 CC denotes and
T934 374-382 NN denotes BG083791
T935 383-386 IN denotes for
T936 387-392 NN denotes Acdp4
T937 392-393 . denotes .
T938 393-459 sentence denotes A modified oligo-dT with a M13 tail was used for the RT reaction.
T939 394-395 DT denotes A
T941 396-404 VBN denotes modified
T942 405-410 NN denotes oligo
T943 410-411 HYPH denotes -
T940 411-413 NN denotes dT
T945 414-418 IN denotes with
T946 419-420 DT denotes a
T948 421-424 NN denotes M13
T947 425-429 NN denotes tail
T949 430-433 VBD denotes was
T944 434-438 VBN denotes used
T950 439-442 IN denotes for
T951 443-446 DT denotes the
T953 447-449 NN denotes RT
T952 450-458 NN denotes reaction
T954 458-459 . denotes .
T955 459-627 sentence denotes A forward primer from each EST marker and the M13 primer (olig-dT tail) were used to amplify the 3' UTR sequence for each corresponding Acdp gene from the RT products.
T956 460-461 DT denotes A
T958 462-469 JJ denotes forward
T957 470-476 NN denotes primer
T960 477-481 IN denotes from
T961 482-486 DT denotes each
T963 487-490 NN denotes EST
T962 491-497 NN denotes marker
T964 498-501 CC denotes and
T965 502-505 DT denotes the
T967 506-509 NN denotes M13
T966 510-516 NN denotes primer
T968 517-518 -LRB- denotes (
T969 518-522 NN denotes olig
T971 522-523 HYPH denotes -
T970 523-525 NN denotes dT
T972 526-530 NN denotes tail
T973 530-531 -RRB- denotes )
T974 532-536 VBD denotes were
T959 537-541 VBN denotes used
T975 542-544 TO denotes to
T976 545-552 VB denotes amplify
T977 553-556 DT denotes the
T979 557-558 CD denotes 3
T981 558-559 SYM denotes '
T980 560-563 NN denotes UTR
T978 564-572 NN denotes sequence
T982 573-576 IN denotes for
T983 577-581 DT denotes each
T985 582-595 VBG denotes corresponding
T986 596-600 NN denotes Acdp
T984 601-605 NN denotes gene
T987 606-610 IN denotes from
T988 611-614 DT denotes the
T990 615-617 NN denotes RT
T989 618-626 NNS denotes products
T991 626-627 . denotes .
T992 627-760 sentence denotes To obtain 5'-end coding sequences for the Acdp genes, we conducted a series nested PCR with combinations of mouse and human primers.
T993 628-630 TO denotes To
T994 631-637 VB denotes obtain
T996 638-639 CD denotes 5
T998 639-640 SYM denotes '
T999 640-641 HYPH denotes -
T997 641-644 NN denotes end
T1001 645-651 VBG denotes coding
T1000 652-661 NNS denotes sequences
T1002 662-665 IN denotes for
T1003 666-669 DT denotes the
T1005 670-674 NN denotes Acdp
T1004 675-680 NNS denotes genes
T1006 680-682 , denotes ,
T1007 682-684 PRP denotes we
T995 685-694 VBD denotes conducted
T1008 695-696 DT denotes a
T1010 697-703 NN denotes series
T1011 704-710 VBN denotes nested
T1009 711-714 NN denotes PCR
T1012 715-719 IN denotes with
T1013 720-732 NNS denotes combinations
T1014 733-735 IN denotes of
T1015 736-741 NN denotes mouse
T1017 742-745 CC denotes and
T1018 746-751 JJ denotes human
T1016 752-759 NNS denotes primers
T1019 759-760 . denotes .
T1020 760-869 sentence denotes The 5' UTR sequences were identified by directly sequencing BAC DNA containing the corresponding Acdp genes.
T1021 761-764 DT denotes The
T1023 765-766 CD denotes 5
T1024 766-767 SYM denotes '
T1025 768-771 NN denotes UTR
T1022 772-781 NNS denotes sequences
T1027 782-786 VBD denotes were
T1026 787-797 VBN denotes identified
T1028 798-800 IN denotes by
T1029 801-809 RB denotes directly
T1030 810-820 VBG denotes sequencing
T1031 821-824 NN denotes BAC
T1032 825-828 NN denotes DNA
T1033 829-839 VBG denotes containing
T1034 840-843 DT denotes the
T1036 844-857 VBG denotes corresponding
T1037 858-862 NN denotes Acdp
T1035 863-868 NNS denotes genes
T1038 868-869 . denotes .
T1039 869-963 sentence denotes The BAC clones were identified by screening a CITB mouse BAC DNA library (Research Genetics).
T1040 870-873 DT denotes The
T1042 874-877 NN denotes BAC
T1041 878-884 NNS denotes clones
T1044 885-889 VBD denotes were
T1043 890-900 VBN denotes identified
T1045 901-903 IN denotes by
T1046 904-913 VBG denotes screening
T1047 914-915 DT denotes a
T1049 916-920 NN denotes CITB
T1051 921-926 NN denotes mouse
T1052 927-930 NN denotes BAC
T1050 931-934 NN denotes DNA
T1048 935-942 NN denotes library
T1053 943-944 -LRB- denotes (
T1055 944-952 NNP denotes Research
T1054 953-961 NNP denotes Genetics
T1056 961-962 -RRB- denotes )
T1057 962-963 . denotes .
T1058 963-1038 sentence denotes The 5' UTR sequences obtained from above were further confirmed by RT-PCR.
T1059 964-967 DT denotes The
T1061 968-969 CD denotes 5
T1062 969-970 SYM denotes '
T1063 971-974 NN denotes UTR
T1060 975-984 NNS denotes sequences
T1065 985-993 VBN denotes obtained
T1066 994-998 IN denotes from
T1067 999-1004 RB denotes above
T1068 1005-1009 VBD denotes were
T1069 1010-1017 RB denotes further
T1064 1018-1027 VBN denotes confirmed
T1070 1028-1030 IN denotes by
T1071 1031-1033 NN denotes RT
T1073 1033-1034 HYPH denotes -
T1072 1034-1037 NN denotes PCR
T1074 1037-1038 . denotes .
T1075 1038-1153 sentence denotes The Acdp1 gene contains 3,631 bp of nucleotide sequence and encodes a predicted protein with 951 amino acids (AA).
T1076 1039-1042 DT denotes The
T1078 1043-1048 NN denotes Acdp1
T1077 1049-1053 NN denotes gene
T1079 1054-1062 VBZ denotes contains
T1080 1063-1068 CD denotes 3,631
T1081 1069-1071 NNS denotes bp
T1082 1072-1074 IN denotes of
T1083 1075-1085 NN denotes nucleotide
T1084 1086-1094 NN denotes sequence
T1085 1095-1098 CC denotes and
T1086 1099-1106 VBZ denotes encodes
T1087 1107-1108 DT denotes a
T1089 1109-1118 VBN denotes predicted
T1088 1119-1126 NN denotes protein
T1090 1127-1131 IN denotes with
T1091 1132-1135 CD denotes 951
T1093 1136-1141 NN denotes amino
T1092 1142-1147 NNS denotes acids
T1094 1148-1149 -LRB- denotes (
T1095 1149-1151 NN denotes AA
T1096 1151-1152 -RRB- denotes )
T1097 1152-1153 . denotes .
T1098 1153-1355 sentence denotes The other three Acdp genes (Acdp2, 3 and 4) contain 3,244 bp, 2,684 bp and 2,743 bp of cDNA sequences, and encode deduced proteins of 874 amino acids, 713 amino acids and 771 amino acids, respectively.
T1099 1154-1157 DT denotes The
T1101 1158-1163 JJ denotes other
T1102 1164-1169 CD denotes three
T1103 1170-1174 NN denotes Acdp
T1100 1175-1180 NNS denotes genes
T1105 1181-1182 -LRB- denotes (
T1106 1182-1187 NN denotes Acdp2
T1107 1187-1189 , denotes ,
T1108 1189-1190 CD denotes 3
T1109 1191-1194 CC denotes and
T1110 1195-1196 CD denotes 4
T1111 1196-1197 -RRB- denotes )
T1104 1198-1205 VBP denotes contain
T1112 1206-1211 CD denotes 3,244
T1113 1212-1214 NNS denotes bp
T1114 1214-1216 , denotes ,
T1115 1216-1221 CD denotes 2,684
T1116 1222-1224 NNS denotes bp
T1117 1225-1228 CC denotes and
T1118 1229-1234 CD denotes 2,743
T1119 1235-1237 NNS denotes bp
T1120 1238-1240 IN denotes of
T1121 1241-1245 NN denotes cDNA
T1122 1246-1255 NNS denotes sequences
T1123 1255-1257 , denotes ,
T1124 1257-1260 CC denotes and
T1125 1261-1267 VBP denotes encode
T1126 1268-1275 VBN denotes deduced
T1127 1276-1284 NN denotes proteins
T1128 1285-1287 IN denotes of
T1129 1288-1291 CD denotes 874
T1131 1292-1297 NN denotes amino
T1130 1298-1303 NNS denotes acids
T1132 1303-1305 , denotes ,
T1133 1305-1308 CD denotes 713
T1135 1309-1314 NN denotes amino
T1134 1315-1320 NNS denotes acids
T1136 1321-1324 CC denotes and
T1137 1325-1328 CD denotes 771
T1139 1329-1334 NN denotes amino
T1138 1335-1340 NNS denotes acids
T1140 1340-1342 , denotes ,
T1141 1342-1354 RB denotes respectively
T1142 1354-1355 . denotes .
R388 T863 T864 amod Molecular,cloning
R389 T865 T864 prep of,cloning
R390 T866 T867 det the,family
R391 T867 T865 pobj family,of
R392 T868 T867 compound Acdp,family
R393 T869 T867 compound gene,family
R394 T871 T872 aux To,clone
R395 T872 T873 advcl clone,used
R396 T874 T875 det the,genes
R397 T875 T872 dobj genes,clone
R398 T876 T875 compound mouse,genes
R399 T877 T875 compound Acdp,genes
R400 T878 T873 punct ", ",used
R401 T879 T880 det the,cDNA
R402 T880 T873 nsubjpass cDNA,used
R403 T881 T880 amod human,cDNA
R404 T882 T880 compound ACDP,cDNA
R405 T883 T880 cc and,cDNA
R406 T884 T885 amod predicted,sequences
R407 T885 T880 conj sequences,cDNA
R408 T886 T885 compound protein,sequences
R409 T887 T873 auxpass were,used
R410 T888 T889 aux to,search
R411 T889 T873 advcl search,used
R412 T890 T891 det the,database
R413 T891 T889 dobj database,search
R414 T892 T891 compound mouse,database
R415 T893 T891 compound EST,database
R416 T894 T889 prep with,search
R417 T895 T896 det the,programs
R418 T896 T894 pobj programs,with
R419 T897 T896 nmod blastn,programs
R420 T898 T897 cc and,blastn
R421 T899 T897 conj tblastn,blastn
R422 T900 T873 punct .,used
R423 T902 T903 compound Mouse,markers
R424 T903 T905 nsubjpass markers,identified
R425 T904 T903 compound EST,markers
R426 T906 T903 acl corresponding,markers
R427 T907 T906 prep to,corresponding
R428 T908 T909 det each,member
R429 T909 T907 pobj member,to
R430 T910 T909 compound Acdp,member
R431 T911 T905 auxpass were,identified
R432 T912 T905 advmod then,identified
R433 T913 T905 punct .,identified
R434 T915 T916 prep For,corresponds
R435 T917 T915 pobj example,For
R436 T918 T916 punct ", ",corresponds
R437 T919 T920 compound EST,H3086H12
R438 T920 T916 nsubj H3086H12,corresponds
R439 T921 T920 punct -,H3086H12
R440 T922 T920 nummod 5,H3086H12
R441 T923 T916 prep to,corresponds
R442 T924 T923 pobj Acdp1,to
R443 T925 T916 punct ", ",corresponds
R444 T926 T916 conj W98010,corresponds
R445 T927 T926 prep for,W98010
R446 T928 T927 pobj Acdp2,for
R447 T929 T926 punct ", ",W98010
R448 T930 T926 conj 603299135F1,W98010
R449 T931 T930 prep for,603299135F1
R450 T932 T931 pobj Acdp3,for
R451 T933 T930 cc and,603299135F1
R452 T934 T930 conj BG083791,603299135F1
R453 T935 T934 prep for,BG083791
R454 T936 T935 pobj Acdp4,for
R455 T937 T916 punct .,corresponds
R456 T939 T940 det A,dT
R457 T940 T944 nsubjpass dT,used
R458 T941 T940 amod modified,dT
R459 T942 T940 compound oligo,dT
R460 T943 T940 punct -,dT
R461 T945 T940 prep with,dT
R462 T946 T947 det a,tail
R463 T947 T945 pobj tail,with
R464 T948 T947 compound M13,tail
R465 T949 T944 auxpass was,used
R466 T950 T944 prep for,used
R467 T951 T952 det the,reaction
R468 T952 T950 pobj reaction,for
R469 T953 T952 compound RT,reaction
R470 T954 T944 punct .,used
R471 T956 T957 det A,primer
R472 T957 T959 nsubjpass primer,used
R473 T958 T957 amod forward,primer
R474 T960 T957 prep from,primer
R475 T961 T962 det each,marker
R476 T962 T960 pobj marker,from
R477 T963 T962 compound EST,marker
R478 T964 T962 cc and,marker
R479 T965 T966 det the,primer
R480 T966 T962 conj primer,marker
R481 T967 T966 compound M13,primer
R482 T968 T966 punct (,primer
R483 T969 T970 compound olig,dT
R484 T970 T972 compound dT,tail
R485 T971 T970 punct -,dT
R486 T972 T966 appos tail,primer
R487 T973 T966 punct ),primer
R488 T974 T959 auxpass were,used
R489 T975 T976 aux to,amplify
R490 T976 T959 advcl amplify,used
R491 T977 T978 det the,sequence
R492 T978 T976 dobj sequence,amplify
R493 T979 T980 nummod 3,UTR
R494 T980 T978 compound UTR,sequence
R495 T981 T979 punct ',3
R496 T982 T976 prep for,amplify
R497 T983 T984 det each,gene
R498 T984 T982 pobj gene,for
R499 T985 T984 amod corresponding,gene
R500 T986 T984 compound Acdp,gene
R501 T987 T984 prep from,gene
R502 T988 T989 det the,products
R503 T989 T987 pobj products,from
R504 T990 T989 compound RT,products
R505 T991 T959 punct .,used
R506 T993 T994 aux To,obtain
R507 T994 T995 advcl obtain,conducted
R508 T996 T997 nummod 5,end
R509 T997 T1000 nmod end,sequences
R510 T998 T996 punct ',5
R511 T999 T997 punct -,end
R512 T1000 T994 dobj sequences,obtain
R513 T1001 T1000 amod coding,sequences
R514 T1002 T994 prep for,obtain
R515 T1003 T1004 det the,genes
R516 T1004 T1002 pobj genes,for
R517 T1005 T1004 compound Acdp,genes
R518 T1006 T995 punct ", ",conducted
R519 T1007 T995 nsubj we,conducted
R520 T1008 T1009 det a,PCR
R521 T1009 T995 dobj PCR,conducted
R522 T1010 T1011 npadvmod series,nested
R523 T1011 T1009 amod nested,PCR
R524 T1012 T995 prep with,conducted
R525 T1013 T1012 pobj combinations,with
R526 T1014 T1013 prep of,combinations
R527 T1015 T1016 nmod mouse,primers
R528 T1016 T1014 pobj primers,of
R529 T1017 T1015 cc and,mouse
R530 T1018 T1015 conj human,mouse
R531 T1019 T995 punct .,conducted
R532 T1021 T1022 det The,sequences
R533 T1022 T1026 nsubjpass sequences,identified
R534 T1023 T1022 nummod 5,sequences
R535 T1024 T1023 punct ',5
R536 T1025 T1022 compound UTR,sequences
R537 T1027 T1026 auxpass were,identified
R538 T1028 T1026 prep by,identified
R539 T1029 T1030 advmod directly,sequencing
R540 T1030 T1028 pcomp sequencing,by
R541 T1031 T1032 compound BAC,DNA
R542 T1032 T1030 dobj DNA,sequencing
R543 T1033 T1032 acl containing,DNA
R544 T1034 T1035 det the,genes
R545 T1035 T1033 dobj genes,containing
R546 T1036 T1035 amod corresponding,genes
R547 T1037 T1035 compound Acdp,genes
R548 T1038 T1026 punct .,identified
R549 T1040 T1041 det The,clones
R550 T1041 T1043 nsubjpass clones,identified
R551 T1042 T1041 compound BAC,clones
R552 T1044 T1043 auxpass were,identified
R553 T1045 T1043 prep by,identified
R554 T1046 T1045 pcomp screening,by
R555 T1047 T1048 det a,library
R556 T1048 T1046 dobj library,screening
R557 T1049 T1050 compound CITB,DNA
R558 T1050 T1048 compound DNA,library
R559 T1051 T1050 compound mouse,DNA
R560 T1052 T1050 compound BAC,DNA
R561 T1053 T1054 punct (,Genetics
R562 T1054 T1048 parataxis Genetics,library
R563 T1055 T1054 compound Research,Genetics
R564 T1056 T1054 punct ),Genetics
R565 T1057 T1043 punct .,identified
R566 T1059 T1060 det The,sequences
R567 T1060 T1064 nsubjpass sequences,confirmed
R568 T1061 T1060 nummod 5,sequences
R569 T1062 T1061 punct ',5
R570 T1063 T1060 compound UTR,sequences
R571 T1065 T1060 acl obtained,sequences
R572 T1066 T1065 prep from,obtained
R573 T1067 T1066 pcomp above,from
R574 T1068 T1064 auxpass were,confirmed
R575 T1069 T1064 advmod further,confirmed
R576 T1070 T1064 prep by,confirmed
R577 T1071 T1072 compound RT,PCR
R578 T1072 T1070 pobj PCR,by
R579 T1073 T1072 punct -,PCR
R580 T1074 T1064 punct .,confirmed
R581 T1076 T1077 det The,gene
R582 T1077 T1079 nsubj gene,contains
R583 T1078 T1077 compound Acdp1,gene
R584 T1080 T1081 nummod "3,631",bp
R585 T1081 T1079 dobj bp,contains
R586 T1082 T1081 prep of,bp
R587 T1083 T1084 compound nucleotide,sequence
R588 T1084 T1082 pobj sequence,of
R589 T1085 T1079 cc and,contains
R590 T1086 T1079 conj encodes,contains
R591 T1087 T1088 det a,protein
R592 T1088 T1086 dobj protein,encodes
R593 T1089 T1088 amod predicted,protein
R594 T1090 T1086 prep with,encodes
R595 T1091 T1092 nummod 951,acids
R596 T1092 T1090 pobj acids,with
R597 T1093 T1092 compound amino,acids
R598 T1094 T1095 punct (,AA
R599 T1095 T1086 parataxis AA,encodes
R600 T1096 T1095 punct ),AA
R601 T1097 T1079 punct .,contains
R602 T1099 T1100 det The,genes
R603 T1100 T1104 nsubj genes,contain
R604 T1101 T1100 amod other,genes
R605 T1102 T1100 nummod three,genes
R606 T1103 T1100 compound Acdp,genes
R607 T1105 T1100 punct (,genes
R608 T1106 T1100 appos Acdp2,genes
R609 T1107 T1106 punct ", ",Acdp2
R610 T1108 T1106 nummod 3,Acdp2
R611 T1109 T1106 cc and,Acdp2
R612 T1110 T1106 conj 4,Acdp2
R613 T1111 T1100 punct ),genes
R614 T1112 T1113 nummod "3,244",bp
R615 T1113 T1104 dobj bp,contain
R616 T1114 T1113 punct ", ",bp
R617 T1115 T1116 nummod "2,684",bp
R618 T1116 T1113 conj bp,bp
R619 T1117 T1116 cc and,bp
R620 T1118 T1119 nummod "2,743",bp
R621 T1119 T1116 conj bp,bp
R622 T1120 T1113 prep of,bp
R623 T1121 T1122 compound cDNA,sequences
R624 T1122 T1120 pobj sequences,of
R625 T1123 T1104 punct ", ",contain
R626 T1124 T1104 cc and,contain
R627 T1125 T1104 conj encode,contain
R628 T1126 T1127 amod deduced,proteins
R629 T1127 T1125 dobj proteins,encode
R630 T1128 T1127 prep of,proteins
R631 T1129 T1130 nummod 874,acids
R632 T1130 T1128 pobj acids,of
R633 T1131 T1130 compound amino,acids
R634 T1132 T1130 punct ", ",acids
R635 T1133 T1134 nummod 713,acids
R636 T1134 T1130 conj acids,acids
R637 T1135 T1134 compound amino,acids
R638 T1136 T1134 cc and,acids
R639 T1137 T1138 nummod 771,acids
R640 T1138 T1134 conj acids,acids
R641 T1139 T1138 compound amino,acids
R642 T1140 T1125 punct ", ",encode
R643 T1141 T1125 advmod respectively,encode
R644 T1142 T1104 punct .,contain