PMC:340383 / 22120-23115 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5450 0-3 IN denotes For
T5452 4-18 NN denotes immunostaining
T5453 18-20 , denotes ,
T5454 20-28 JJ denotes neuronal
T5455 29-34 NNS denotes cells
T5456 35-37 IN denotes on
T5457 38-41 DT denotes the
T5458 42-52 NNS denotes coverslips
T5459 53-57 VBD denotes were
T5460 58-63 RB denotes first
T5451 64-69 VBN denotes fixed
T5461 70-72 IN denotes in
T5462 73-76 NN denotes PBS
T5463 77-87 VBG denotes containing
T5464 88-89 CD denotes 4
T5465 89-90 NN denotes %
T5466 91-107 NN denotes paraformaldehyde
T5467 108-109 -LRB- denotes (
T5468 109-112 NN denotes PFA
T5469 112-113 -RRB- denotes )
T5470 114-117 IN denotes for
T5471 118-120 CD denotes 12
T5472 121-124 NNS denotes hrs
T5473 125-127 IN denotes at
T5474 128-129 CD denotes 4
T5475 129-131 NN denotes °C
T5476 132-135 CC denotes and
T5477 136-140 RB denotes then
T5478 141-150 VBN denotes incubated
T5479 151-153 IN denotes in
T5480 154-155 DT denotes a
T5481 156-164 NN denotes solution
T5482 165-175 VBG denotes containing
T5483 176-177 CD denotes 4
T5484 177-178 NN denotes %
T5485 179-182 NN denotes PFA
T5486 183-186 CC denotes and
T5487 187-190 CD denotes 0.4
T5488 190-191 NN denotes %
T5490 192-198 NN denotes Triton
T5491 199-200 NN denotes X
T5492 200-201 HYPH denotes -
T5489 201-204 NN denotes 100
T5493 205-207 IN denotes at
T5494 208-209 CD denotes 4
T5495 209-211 NN denotes °C
T5496 212-215 IN denotes for
T5497 216-217 CD denotes 1
T5498 218-220 NN denotes hr
T5499 220-221 . denotes .
T5500 221-440 sentence denotes After washing with PBS three times, the cells were incubated with a blocking solution containing 1:30 normal goat serum, and subsequently incubated with a rabbit polyclonal anti-ACDP antibody (1:3000) overnight at 4°C.
T5501 222-227 IN denotes After
T5503 228-235 VBG denotes washing
T5504 236-240 IN denotes with
T5505 241-244 NN denotes PBS
T5506 245-250 CD denotes three
T5507 251-256 NNS denotes times
T5508 256-258 , denotes ,
T5509 258-261 DT denotes the
T5510 262-267 NNS denotes cells
T5511 268-272 VBD denotes were
T5502 273-282 VBN denotes incubated
T5512 283-287 IN denotes with
T5513 288-289 DT denotes a
T5515 290-298 VBG denotes blocking
T5514 299-307 NN denotes solution
T5516 308-318 VBG denotes containing
T5517 319-320 CD denotes 1
T5519 320-321 SYM denotes :
T5520 321-323 CD denotes 30
T5521 324-330 JJ denotes normal
T5522 331-335 NN denotes goat
T5518 336-341 NN denotes serum
T5523 341-343 , denotes ,
T5524 343-346 CC denotes and
T5525 347-359 RB denotes subsequently
T5526 360-369 VBN denotes incubated
T5527 370-374 IN denotes with
T5528 375-376 DT denotes a
T5530 377-383 NN denotes rabbit
T5531 384-394 JJ denotes polyclonal
T5532 395-404 JJ denotes anti-ACDP
T5529 405-413 NN denotes antibody
T5533 414-415 -LRB- denotes (
T5534 415-416 CD denotes 1
T5535 416-417 SYM denotes :
T5536 417-421 CD denotes 3000
T5537 421-422 -RRB- denotes )
T5538 423-432 RB denotes overnight
T5539 433-435 IN denotes at
T5540 436-437 CD denotes 4
T5541 437-439 NN denotes °C
T5542 439-440 . denotes .
T5543 440-656 sentence denotes After extensive washing with 1% goat serum PBS solution, the cells were incubated with an Alex 488 conjugated secondary antibody (1:100 in 1% goat serum PBS solution, Molecular Probes) for 3 hrs at room temperature.
T5544 441-446 IN denotes After
T5546 447-456 JJ denotes extensive
T5547 457-464 NN denotes washing
T5548 465-469 IN denotes with
T5549 470-471 CD denotes 1
T5550 471-472 NN denotes %
T5552 473-477 NN denotes goat
T5551 478-483 NN denotes serum
T5554 484-487 NN denotes PBS
T5553 488-496 NN denotes solution
T5555 496-498 , denotes ,
T5556 498-501 DT denotes the
T5557 502-507 NNS denotes cells
T5558 508-512 VBD denotes were
T5545 513-522 VBN denotes incubated
T5559 523-527 IN denotes with
T5560 528-530 DT denotes an
T5562 531-535 NNP denotes Alex
T5563 536-539 CD denotes 488
T5564 540-550 JJ denotes conjugated
T5565 551-560 JJ denotes secondary
T5561 561-569 NN denotes antibody
T5566 570-571 -LRB- denotes (
T5568 571-572 CD denotes 1
T5569 572-573 SYM denotes :
T5570 573-576 CD denotes 100
T5571 577-579 IN denotes in
T5572 580-581 CD denotes 1
T5573 581-582 NN denotes %
T5575 583-587 NN denotes goat
T5574 588-593 NN denotes serum
T5577 594-597 NN denotes PBS
T5576 598-606 NN denotes solution
T5578 606-608 , denotes ,
T5579 608-617 NNP denotes Molecular
T5567 618-624 NNP denotes Probes
T5580 624-625 -RRB- denotes )
T5581 626-629 IN denotes for
T5582 630-631 CD denotes 3
T5583 632-635 NNS denotes hrs
T5584 636-638 IN denotes at
T5585 639-643 NN denotes room
T5586 644-655 NN denotes temperature
T5587 655-656 . denotes .
T5588 656-815 sentence denotes Following final washes with 1% goat serum PBS solution, the neuronal cells on the coverslips were cover-slipped with a glycerol-based anti-photobleach medium.
T5589 657-666 IN denotes Following
T5591 667-672 JJ denotes final
T5592 673-679 NNS denotes washes
T5593 680-684 IN denotes with
T5594 685-686 CD denotes 1
T5595 686-687 NN denotes %
T5597 688-692 NN denotes goat
T5596 693-698 NN denotes serum
T5599 699-702 NN denotes PBS
T5598 703-711 NN denotes solution
T5600 711-713 , denotes ,
T5601 713-716 DT denotes the
T5603 717-725 JJ denotes neuronal
T5602 726-731 NNS denotes cells
T5604 732-734 IN denotes on
T5605 735-738 DT denotes the
T5606 739-749 NNS denotes coverslips
T5607 750-754 VBD denotes were
T5608 755-760 NN denotes cover
T5609 760-761 HYPH denotes -
T5590 761-768 VBN denotes slipped
T5610 769-773 IN denotes with
T5611 774-775 DT denotes a
T5613 776-784 NN denotes glycerol
T5615 784-785 HYPH denotes -
T5614 785-790 VBN denotes based
T5616 791-807 JJ denotes anti-photobleach
T5612 808-814 NN denotes medium
T5617 814-815 . denotes .
T5618 815-879 sentence denotes The cells were viewed under a confocal microscope (Carl Zeiss).
T5619 816-819 DT denotes The
T5620 820-825 NNS denotes cells
T5622 826-830 VBD denotes were
T5621 831-837 VBN denotes viewed
T5623 838-843 IN denotes under
T5624 844-845 DT denotes a
T5626 846-854 JJ denotes confocal
T5625 855-865 NN denotes microscope
T5627 866-867 -LRB- denotes (
T5629 867-871 NNP denotes Carl
T5628 872-877 NNP denotes Zeiss
T5630 877-878 -RRB- denotes )
T5631 878-879 . denotes .
T5632 879-995 sentence denotes Images were captured with a CCD camera and acquired by the Scion Image software (Scion Corporation, Frederick, MD).
T5633 880-886 NNS denotes Images
T5635 887-891 VBD denotes were
T5634 892-900 VBN denotes captured
T5636 901-905 IN denotes with
T5637 906-907 DT denotes a
T5639 908-911 NN denotes CCD
T5638 912-918 NN denotes camera
T5640 919-922 CC denotes and
T5641 923-931 VBN denotes acquired
T5642 932-934 IN denotes by
T5643 935-938 DT denotes the
T5645 939-944 NNP denotes Scion
T5646 945-950 NN denotes Image
T5644 951-959 NN denotes software
T5647 960-961 -LRB- denotes (
T5649 961-966 NNP denotes Scion
T5648 967-978 NNP denotes Corporation
T5650 978-980 , denotes ,
T5651 980-989 NNP denotes Frederick
T5652 989-991 , denotes ,
T5653 991-993 NNP denotes MD
T5654 993-994 -RRB- denotes )
T5655 994-995 . denotes .
R3309 T5450 T5451 prep For,fixed
R3310 T5452 T5450 pobj immunostaining,For
R3311 T5453 T5451 punct ", ",fixed
R3312 T5454 T5455 amod neuronal,cells
R3313 T5455 T5451 nsubjpass cells,fixed
R3314 T5456 T5455 prep on,cells
R3315 T5457 T5458 det the,coverslips
R3316 T5458 T5456 pobj coverslips,on
R3317 T5459 T5451 auxpass were,fixed
R3318 T5460 T5451 advmod first,fixed
R3319 T5461 T5451 prep in,fixed
R3320 T5462 T5461 pobj PBS,in
R3321 T5463 T5462 acl containing,PBS
R3322 T5464 T5465 nummod 4,%
R3323 T5465 T5466 compound %,paraformaldehyde
R3324 T5466 T5463 dobj paraformaldehyde,containing
R3325 T5467 T5466 punct (,paraformaldehyde
R3326 T5468 T5466 appos PFA,paraformaldehyde
R3327 T5469 T5463 punct ),containing
R3328 T5470 T5463 prep for,containing
R3329 T5471 T5472 nummod 12,hrs
R3330 T5472 T5470 pobj hrs,for
R3331 T5473 T5463 prep at,containing
R3332 T5474 T5475 nummod 4,°C
R3333 T5475 T5473 pobj °C,at
R3334 T5476 T5451 cc and,fixed
R3335 T5477 T5478 advmod then,incubated
R3336 T5478 T5451 conj incubated,fixed
R3337 T5479 T5478 prep in,incubated
R3338 T5480 T5481 det a,solution
R3339 T5481 T5479 pobj solution,in
R3340 T5482 T5481 acl containing,solution
R3341 T5483 T5484 nummod 4,%
R3342 T5484 T5485 compound %,PFA
R3343 T5485 T5482 dobj PFA,containing
R3344 T5486 T5485 cc and,PFA
R3345 T5487 T5488 nummod 0.4,%
R3346 T5488 T5489 compound %,100
R3347 T5489 T5485 conj 100,PFA
R3348 T5490 T5489 compound Triton,100
R3349 T5491 T5489 compound X,100
R3350 T5492 T5489 punct -,100
R3351 T5493 T5478 prep at,incubated
R3352 T5494 T5495 nummod 4,°C
R3353 T5495 T5493 pobj °C,at
R3354 T5496 T5478 prep for,incubated
R3355 T5497 T5498 nummod 1,hr
R3356 T5498 T5496 pobj hr,for
R3357 T5499 T5451 punct .,fixed
R3358 T5501 T5502 prep After,incubated
R3359 T5503 T5501 pcomp washing,After
R3360 T5504 T5503 prep with,washing
R3361 T5505 T5504 pobj PBS,with
R3362 T5506 T5507 nummod three,times
R3363 T5507 T5503 npadvmod times,washing
R3364 T5508 T5502 punct ", ",incubated
R3365 T5509 T5510 det the,cells
R3366 T5510 T5502 nsubjpass cells,incubated
R3367 T5511 T5502 auxpass were,incubated
R3368 T5512 T5502 prep with,incubated
R3369 T5513 T5514 det a,solution
R3370 T5514 T5512 pobj solution,with
R3371 T5515 T5514 amod blocking,solution
R3372 T5516 T5514 acl containing,solution
R3373 T5517 T5518 nummod 1,serum
R3374 T5518 T5516 dobj serum,containing
R3375 T5519 T5520 punct :,30
R3376 T5520 T5517 prep 30,1
R3377 T5521 T5518 amod normal,serum
R3378 T5522 T5518 compound goat,serum
R3379 T5523 T5502 punct ", ",incubated
R3380 T5524 T5502 cc and,incubated
R3381 T5525 T5526 advmod subsequently,incubated
R3382 T5526 T5502 conj incubated,incubated
R3383 T5527 T5526 prep with,incubated
R3384 T5528 T5529 det a,antibody
R3385 T5529 T5527 pobj antibody,with
R3386 T5530 T5529 nmod rabbit,antibody
R3387 T5531 T5529 amod polyclonal,antibody
R3388 T5532 T5529 amod anti-ACDP,antibody
R3389 T5533 T5534 punct (,1
R3390 T5534 T5529 parataxis 1,antibody
R3391 T5535 T5536 punct :,3000
R3392 T5536 T5534 prep 3000,1
R3393 T5537 T5534 punct ),1
R3394 T5538 T5526 advmod overnight,incubated
R3395 T5539 T5526 prep at,incubated
R3396 T5540 T5541 nummod 4,°C
R3397 T5541 T5539 pobj °C,at
R3398 T5542 T5502 punct .,incubated
R3399 T5544 T5545 prep After,incubated
R3400 T5546 T5547 amod extensive,washing
R3401 T5547 T5544 pobj washing,After
R3402 T5548 T5547 prep with,washing
R3403 T5549 T5550 nummod 1,%
R3404 T5550 T5551 compound %,serum
R3405 T5551 T5553 compound serum,solution
R3406 T5552 T5551 compound goat,serum
R3407 T5553 T5548 pobj solution,with
R3408 T5554 T5553 compound PBS,solution
R3409 T5555 T5545 punct ", ",incubated
R3410 T5556 T5557 det the,cells
R3411 T5557 T5545 nsubjpass cells,incubated
R3412 T5558 T5545 auxpass were,incubated
R3413 T5559 T5545 prep with,incubated
R3414 T5560 T5561 det an,antibody
R3415 T5561 T5559 pobj antibody,with
R3416 T5562 T5561 nmod Alex,antibody
R3417 T5563 T5562 nummod 488,Alex
R3418 T5564 T5561 amod conjugated,antibody
R3419 T5565 T5561 amod secondary,antibody
R3420 T5566 T5567 punct (,Probes
R3421 T5567 T5561 parataxis Probes,antibody
R3422 T5568 T5567 dep 1,Probes
R3423 T5569 T5570 punct :,100
R3424 T5570 T5568 prep 100,1
R3425 T5571 T5568 prep in,1
R3426 T5572 T5573 nummod 1,%
R3427 T5573 T5574 compound %,serum
R3428 T5574 T5576 compound serum,solution
R3429 T5575 T5574 compound goat,serum
R3430 T5576 T5571 pobj solution,in
R3431 T5577 T5576 compound PBS,solution
R3432 T5578 T5567 punct ", ",Probes
R3433 T5579 T5567 compound Molecular,Probes
R3434 T5580 T5567 punct ),Probes
R3435 T5581 T5545 prep for,incubated
R3436 T5582 T5583 nummod 3,hrs
R3437 T5583 T5581 pobj hrs,for
R3438 T5584 T5545 prep at,incubated
R3439 T5585 T5586 compound room,temperature
R3440 T5586 T5584 pobj temperature,at
R3441 T5587 T5545 punct .,incubated
R3442 T5589 T5590 prep Following,slipped
R3443 T5591 T5592 amod final,washes
R3444 T5592 T5589 pobj washes,Following
R3445 T5593 T5592 prep with,washes
R3446 T5594 T5595 nummod 1,%
R3447 T5595 T5596 compound %,serum
R3448 T5596 T5598 compound serum,solution
R3449 T5597 T5596 compound goat,serum
R3450 T5598 T5593 pobj solution,with
R3451 T5599 T5598 compound PBS,solution
R3452 T5600 T5590 punct ", ",slipped
R3453 T5601 T5602 det the,cells
R3454 T5602 T5590 nsubjpass cells,slipped
R3455 T5603 T5602 amod neuronal,cells
R3456 T5604 T5602 prep on,cells
R3457 T5605 T5606 det the,coverslips
R3458 T5606 T5604 pobj coverslips,on
R3459 T5607 T5590 auxpass were,slipped
R3460 T5608 T5590 dep cover,slipped
R3461 T5609 T5590 punct -,slipped
R3462 T5610 T5590 prep with,slipped
R3463 T5611 T5612 det a,medium
R3464 T5612 T5610 pobj medium,with
R3465 T5613 T5614 npadvmod glycerol,based
R3466 T5614 T5612 amod based,medium
R3467 T5615 T5614 punct -,based
R3468 T5616 T5612 amod anti-photobleach,medium
R3469 T5617 T5590 punct .,slipped
R3470 T5619 T5620 det The,cells
R3471 T5620 T5621 nsubjpass cells,viewed
R3472 T5622 T5621 auxpass were,viewed
R3473 T5623 T5621 prep under,viewed
R3474 T5624 T5625 det a,microscope
R3475 T5625 T5623 pobj microscope,under
R3476 T5626 T5625 amod confocal,microscope
R3477 T5627 T5628 punct (,Zeiss
R3478 T5628 T5625 parataxis Zeiss,microscope
R3479 T5629 T5628 compound Carl,Zeiss
R3480 T5630 T5628 punct ),Zeiss
R3481 T5631 T5621 punct .,viewed
R3482 T5633 T5634 nsubjpass Images,captured
R3483 T5635 T5634 auxpass were,captured
R3484 T5636 T5634 prep with,captured
R3485 T5637 T5638 det a,camera
R3486 T5638 T5636 pobj camera,with
R3487 T5639 T5638 compound CCD,camera
R3488 T5640 T5634 cc and,captured
R3489 T5641 T5634 conj acquired,captured
R3490 T5642 T5641 prep by,acquired
R3491 T5643 T5644 det the,software
R3492 T5644 T5642 pobj software,by
R3493 T5645 T5644 compound Scion,software
R3494 T5646 T5644 compound Image,software
R3495 T5647 T5648 punct (,Corporation
R3496 T5648 T5644 parataxis Corporation,software
R3497 T5649 T5648 compound Scion,Corporation
R3498 T5650 T5648 punct ", ",Corporation
R3499 T5651 T5648 npadvmod Frederick,Corporation
R3500 T5652 T5648 punct ", ",Corporation
R3501 T5653 T5648 npadvmod MD,Corporation
R3502 T5654 T5648 punct ),Corporation
R3503 T5655 T5634 punct .,captured