| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T5270 |
0-11 |
JJ |
denotes |
Hippocampal |
| T5272 |
12-18 |
NN |
denotes |
neuron |
| T5271 |
19-27 |
NNS |
denotes |
cultures |
| T5274 |
28-32 |
VBD |
denotes |
were |
| T5273 |
33-41 |
VBN |
denotes |
prepared |
| T5275 |
42-44 |
IN |
denotes |
as |
| T5277 |
45-55 |
RB |
denotes |
previously |
| T5276 |
56-64 |
VBN |
denotes |
reported |
| T5278 |
65-66 |
-LRB- |
denotes |
[ |
| T5279 |
66-67 |
CD |
denotes |
6 |
| T5280 |
67-68 |
-RRB- |
denotes |
] |
| T5281 |
68-69 |
. |
denotes |
. |
| T5282 |
69-156 |
sentence |
denotes |
In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero. |
| T5283 |
70-72 |
IN |
denotes |
In |
| T5285 |
73-78 |
NN |
denotes |
brief |
| T5286 |
78-80 |
, |
denotes |
, |
| T5287 |
80-83 |
DT |
denotes |
the |
| T5288 |
84-97 |
NNS |
denotes |
hippocampuses |
| T5289 |
98-102 |
VBD |
denotes |
were |
| T5284 |
103-112 |
VBN |
denotes |
dissected |
| T5290 |
113-116 |
RP |
denotes |
out |
| T5291 |
117-121 |
IN |
denotes |
from |
| T5292 |
122-127 |
NN |
denotes |
mouse |
| T5293 |
128-135 |
NNS |
denotes |
embryos |
| T5294 |
136-138 |
IN |
denotes |
at |
| T5295 |
139-141 |
CD |
denotes |
16 |
| T5296 |
142-146 |
NNS |
denotes |
days |
| T5297 |
147-149 |
FW |
denotes |
in |
| T5298 |
150-155 |
FW |
denotes |
utero |
| T5299 |
155-156 |
. |
denotes |
. |
| T5300 |
156-333 |
sentence |
denotes |
The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies). |
| T5301 |
157-160 |
DT |
denotes |
The |
| T5302 |
161-168 |
NNS |
denotes |
tissues |
| T5304 |
169-173 |
VBD |
denotes |
were |
| T5305 |
174-178 |
RB |
denotes |
then |
| T5303 |
179-188 |
VBN |
denotes |
incubated |
| T5306 |
189-192 |
IN |
denotes |
for |
| T5307 |
193-195 |
CD |
denotes |
20 |
| T5308 |
196-199 |
NN |
denotes |
min |
| T5309 |
200-202 |
IN |
denotes |
at |
| T5310 |
203-205 |
CD |
denotes |
37 |
| T5311 |
205-207 |
NN |
denotes |
°C |
| T5312 |
208-210 |
IN |
denotes |
in |
| T5313 |
211-214 |
NN |
denotes |
MEM |
| T5314 |
215-216 |
-LRB- |
denotes |
( |
| T5315 |
216-223 |
JJ |
denotes |
minimum |
| T5317 |
224-233 |
JJ |
denotes |
essential |
| T5316 |
234-240 |
NN |
denotes |
medium |
| T5318 |
240-241 |
-RRB- |
denotes |
) |
| T5319 |
242-250 |
VBN |
denotes |
modified |
| T5320 |
251-254 |
IN |
denotes |
for |
| T5321 |
255-265 |
NN |
denotes |
suspension |
| T5322 |
266-273 |
NN |
denotes |
culture |
| T5323 |
274-275 |
-LRB- |
denotes |
( |
| T5325 |
275-279 |
NNP |
denotes |
Life |
| T5324 |
280-292 |
NNP |
denotes |
Technologies |
| T5326 |
292-293 |
-RRB- |
denotes |
) |
| T5327 |
294-298 |
CC |
denotes |
plus |
| T5328 |
299-303 |
CD |
denotes |
0.25 |
| T5329 |
303-304 |
NN |
denotes |
% |
| T5330 |
305-312 |
NN |
denotes |
trypsin |
| T5331 |
313-314 |
-LRB- |
denotes |
( |
| T5333 |
314-318 |
NNP |
denotes |
Life |
| T5332 |
319-331 |
NNP |
denotes |
Technologies |
| T5334 |
331-332 |
-RRB- |
denotes |
) |
| T5335 |
332-333 |
. |
denotes |
. |
| T5336 |
333-493 |
sentence |
denotes |
The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below. |
| T5337 |
334-337 |
DT |
denotes |
The |
| T5339 |
338-349 |
JJ |
denotes |
dissociated |
| T5340 |
350-361 |
JJ |
denotes |
hippocampal |
| T5338 |
362-369 |
NNS |
denotes |
neurons |
| T5342 |
370-374 |
VBD |
denotes |
were |
| T5341 |
375-381 |
VBN |
denotes |
plated |
| T5343 |
382-384 |
IN |
denotes |
on |
| T5344 |
385-390 |
NN |
denotes |
glass |
| T5345 |
391-401 |
NNS |
denotes |
coverslips |
| T5346 |
402-408 |
VBN |
denotes |
coated |
| T5347 |
409-413 |
IN |
denotes |
with |
| T5348 |
414-415 |
DT |
denotes |
a |
| T5350 |
416-425 |
JJ |
denotes |
confluent |
| T5349 |
426-435 |
NN |
denotes |
monolayer |
| T5351 |
436-438 |
IN |
denotes |
of |
| T5352 |
439-444 |
NN |
denotes |
mouse |
| T5354 |
445-453 |
JJ |
denotes |
cortical |
| T5353 |
454-464 |
NNS |
denotes |
astrocytes |
| T5355 |
465-473 |
VBN |
denotes |
obtained |
| T5356 |
474-476 |
IN |
denotes |
as |
| T5357 |
477-486 |
VBN |
denotes |
described |
| T5358 |
487-492 |
RB |
denotes |
below |
| T5359 |
492-493 |
. |
denotes |
. |
| T5360 |
493-569 |
sentence |
denotes |
The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2. |
| T5361 |
494-497 |
DT |
denotes |
The |
| T5362 |
498-505 |
NNS |
denotes |
neurons |
| T5364 |
506-510 |
VBD |
denotes |
were |
| T5363 |
511-521 |
VBN |
denotes |
maintained |
| T5365 |
522-524 |
IN |
denotes |
at |
| T5366 |
525-527 |
CD |
denotes |
37 |
| T5367 |
527-529 |
NN |
denotes |
°C |
| T5368 |
530-532 |
IN |
denotes |
in |
| T5369 |
533-534 |
DT |
denotes |
a |
| T5371 |
535-545 |
JJ |
denotes |
humidified |
| T5370 |
546-556 |
NN |
denotes |
atmosphere |
| T5372 |
557-561 |
IN |
denotes |
with |
| T5373 |
562-563 |
CD |
denotes |
5 |
| T5374 |
563-564 |
NN |
denotes |
% |
| T5375 |
565-568 |
NN |
denotes |
CO2 |
| T5376 |
568-569 |
. |
denotes |
. |
| T5377 |
569-722 |
sentence |
denotes |
Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent. |
| T5378 |
570-578 |
JJ |
denotes |
Cortical |
| T5379 |
579-589 |
NNS |
denotes |
astrocytes |
| T5381 |
590-601 |
VBN |
denotes |
dissociated |
| T5382 |
602-606 |
IN |
denotes |
from |
| T5383 |
607-614 |
JJ |
denotes |
newborn |
| T5384 |
615-620 |
NN |
denotes |
mouse |
| T5385 |
621-629 |
NNS |
denotes |
cortices |
| T5386 |
630-634 |
VBD |
denotes |
were |
| T5380 |
635-640 |
VBN |
denotes |
grown |
| T5387 |
641-643 |
IN |
denotes |
in |
| T5388 |
644-651 |
NN |
denotes |
culture |
| T5389 |
652-658 |
NNS |
denotes |
flasks |
| T5390 |
659-661 |
IN |
denotes |
at |
| T5391 |
662-664 |
CD |
denotes |
37 |
| T5392 |
664-666 |
NN |
denotes |
°C |
| T5393 |
667-669 |
IN |
denotes |
in |
| T5394 |
670-671 |
DT |
denotes |
a |
| T5396 |
672-682 |
JJ |
denotes |
humidified |
| T5395 |
683-693 |
NN |
denotes |
atmosphere |
| T5397 |
694-698 |
IN |
denotes |
with |
| T5398 |
699-700 |
CD |
denotes |
5 |
| T5399 |
700-701 |
NN |
denotes |
% |
| T5400 |
702-705 |
NN |
denotes |
CO2 |
| T5401 |
706-711 |
IN |
denotes |
until |
| T5402 |
712-721 |
JJ |
denotes |
confluent |
| T5403 |
721-722 |
. |
denotes |
. |
| T5404 |
722-831 |
sentence |
denotes |
The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C. |
| T5405 |
723-726 |
DT |
denotes |
The |
| T5406 |
727-732 |
NNS |
denotes |
cells |
| T5408 |
733-737 |
VBD |
denotes |
were |
| T5407 |
738-745 |
VBN |
denotes |
exposed |
| T5409 |
746-748 |
IN |
denotes |
to |
| T5410 |
749-751 |
CD |
denotes |
10 |
| T5412 |
751-752 |
SYM |
denotes |
- |
| T5411 |
752-753 |
CD |
denotes |
5 |
| T5413 |
754-755 |
NN |
denotes |
M |
| T5415 |
756-764 |
NN |
denotes |
cytosine |
| T5414 |
765-776 |
NN |
denotes |
arabinoside |
| T5416 |
777-778 |
-LRB- |
denotes |
( |
| T5417 |
778-783 |
NNP |
denotes |
Sigma |
| T5418 |
783-784 |
-RRB- |
denotes |
) |
| T5419 |
785-788 |
CC |
denotes |
and |
| T5420 |
789-797 |
VBN |
denotes |
cultured |
| T5421 |
798-801 |
IN |
denotes |
for |
| T5422 |
802-812 |
JJ |
denotes |
additional |
| T5424 |
813-815 |
CD |
denotes |
12 |
| T5426 |
815-816 |
SYM |
denotes |
– |
| T5425 |
816-818 |
CD |
denotes |
24 |
| T5423 |
819-822 |
NNS |
denotes |
hrs |
| T5427 |
823-825 |
IN |
denotes |
at |
| T5428 |
826-828 |
CD |
denotes |
37 |
| T5429 |
828-830 |
NN |
denotes |
°C |
| T5430 |
830-831 |
. |
denotes |
. |
| T5431 |
831-923 |
sentence |
denotes |
After remove of the media with cellular debris, the cells were used for coating coverslips. |
| T5432 |
832-837 |
IN |
denotes |
After |
| T5434 |
838-844 |
NN |
denotes |
remove |
| T5435 |
845-847 |
IN |
denotes |
of |
| T5436 |
848-851 |
DT |
denotes |
the |
| T5437 |
852-857 |
NNS |
denotes |
media |
| T5438 |
858-862 |
IN |
denotes |
with |
| T5439 |
863-871 |
JJ |
denotes |
cellular |
| T5440 |
872-878 |
NN |
denotes |
debris |
| T5441 |
878-880 |
, |
denotes |
, |
| T5442 |
880-883 |
DT |
denotes |
the |
| T5443 |
884-889 |
NNS |
denotes |
cells |
| T5444 |
890-894 |
VBD |
denotes |
were |
| T5433 |
895-899 |
VBN |
denotes |
used |
| T5445 |
900-903 |
IN |
denotes |
for |
| T5446 |
904-911 |
VBG |
denotes |
coating |
| T5447 |
912-922 |
NNS |
denotes |
coverslips |
| T5448 |
922-923 |
. |
denotes |
. |
| R3145 |
T5270 |
T5271 |
amod |
Hippocampal,cultures |
| R3146 |
T5271 |
T5273 |
nsubjpass |
cultures,prepared |
| R3147 |
T5272 |
T5271 |
compound |
neuron,cultures |
| R3148 |
T5274 |
T5273 |
auxpass |
were,prepared |
| R3149 |
T5275 |
T5276 |
mark |
as,reported |
| R3150 |
T5276 |
T5273 |
advcl |
reported,prepared |
| R3151 |
T5277 |
T5276 |
advmod |
previously,reported |
| R3152 |
T5278 |
T5279 |
punct |
[,6 |
| R3153 |
T5279 |
T5273 |
parataxis |
6,prepared |
| R3154 |
T5280 |
T5279 |
punct |
],6 |
| R3155 |
T5281 |
T5273 |
punct |
.,prepared |
| R3156 |
T5283 |
T5284 |
prep |
In,dissected |
| R3157 |
T5285 |
T5283 |
pobj |
brief,In |
| R3158 |
T5286 |
T5284 |
punct |
", ",dissected |
| R3159 |
T5287 |
T5288 |
det |
the,hippocampuses |
| R3160 |
T5288 |
T5284 |
nsubjpass |
hippocampuses,dissected |
| R3161 |
T5289 |
T5284 |
auxpass |
were,dissected |
| R3162 |
T5290 |
T5284 |
prt |
out,dissected |
| R3163 |
T5291 |
T5284 |
prep |
from,dissected |
| R3164 |
T5292 |
T5293 |
compound |
mouse,embryos |
| R3165 |
T5293 |
T5291 |
pobj |
embryos,from |
| R3166 |
T5294 |
T5284 |
prep |
at,dissected |
| R3167 |
T5295 |
T5296 |
nummod |
16,days |
| R3168 |
T5296 |
T5294 |
pobj |
days,at |
| R3169 |
T5297 |
T5298 |
advmod |
in,utero |
| R3170 |
T5298 |
T5296 |
advmod |
utero,days |
| R3171 |
T5299 |
T5284 |
punct |
.,dissected |
| R3172 |
T5301 |
T5302 |
det |
The,tissues |
| R3173 |
T5302 |
T5303 |
nsubjpass |
tissues,incubated |
| R3174 |
T5304 |
T5303 |
auxpass |
were,incubated |
| R3175 |
T5305 |
T5303 |
advmod |
then,incubated |
| R3176 |
T5306 |
T5303 |
prep |
for,incubated |
| R3177 |
T5307 |
T5308 |
nummod |
20,min |
| R3178 |
T5308 |
T5306 |
pobj |
min,for |
| R3179 |
T5309 |
T5303 |
prep |
at,incubated |
| R3180 |
T5310 |
T5311 |
nummod |
37,°C |
| R3181 |
T5311 |
T5309 |
pobj |
°C,at |
| R3182 |
T5312 |
T5303 |
prep |
in,incubated |
| R3183 |
T5313 |
T5312 |
pobj |
MEM,in |
| R3184 |
T5314 |
T5313 |
punct |
(,MEM |
| R3185 |
T5315 |
T5316 |
amod |
minimum,medium |
| R3186 |
T5316 |
T5313 |
appos |
medium,MEM |
| R3187 |
T5317 |
T5316 |
amod |
essential,medium |
| R3188 |
T5318 |
T5313 |
punct |
),MEM |
| R3189 |
T5319 |
T5313 |
acl |
modified,MEM |
| R3190 |
T5320 |
T5319 |
prep |
for,modified |
| R3191 |
T5321 |
T5322 |
compound |
suspension,culture |
| R3192 |
T5322 |
T5320 |
pobj |
culture,for |
| R3193 |
T5323 |
T5324 |
punct |
(,Technologies |
| R3194 |
T5324 |
T5313 |
parataxis |
Technologies,MEM |
| R3195 |
T5325 |
T5324 |
compound |
Life,Technologies |
| R3196 |
T5326 |
T5324 |
punct |
),Technologies |
| R3197 |
T5327 |
T5313 |
cc |
plus,MEM |
| R3198 |
T5328 |
T5329 |
nummod |
0.25,% |
| R3199 |
T5329 |
T5330 |
compound |
%,trypsin |
| R3200 |
T5330 |
T5313 |
conj |
trypsin,MEM |
| R3201 |
T5331 |
T5332 |
punct |
(,Technologies |
| R3202 |
T5332 |
T5330 |
parataxis |
Technologies,trypsin |
| R3203 |
T5333 |
T5332 |
compound |
Life,Technologies |
| R3204 |
T5334 |
T5332 |
punct |
),Technologies |
| R3205 |
T5335 |
T5303 |
punct |
.,incubated |
| R3206 |
T5337 |
T5338 |
det |
The,neurons |
| R3207 |
T5338 |
T5341 |
nsubjpass |
neurons,plated |
| R3208 |
T5339 |
T5338 |
amod |
dissociated,neurons |
| R3209 |
T5340 |
T5338 |
amod |
hippocampal,neurons |
| R3210 |
T5342 |
T5341 |
auxpass |
were,plated |
| R3211 |
T5343 |
T5341 |
prep |
on,plated |
| R3212 |
T5344 |
T5345 |
compound |
glass,coverslips |
| R3213 |
T5345 |
T5343 |
pobj |
coverslips,on |
| R3214 |
T5346 |
T5345 |
acl |
coated,coverslips |
| R3215 |
T5347 |
T5346 |
prep |
with,coated |
| R3216 |
T5348 |
T5349 |
det |
a,monolayer |
| R3217 |
T5349 |
T5347 |
pobj |
monolayer,with |
| R3218 |
T5350 |
T5349 |
amod |
confluent,monolayer |
| R3219 |
T5351 |
T5349 |
prep |
of,monolayer |
| R3220 |
T5352 |
T5353 |
nmod |
mouse,astrocytes |
| R3221 |
T5353 |
T5351 |
pobj |
astrocytes,of |
| R3222 |
T5354 |
T5353 |
amod |
cortical,astrocytes |
| R3223 |
T5355 |
T5353 |
acl |
obtained,astrocytes |
| R3224 |
T5356 |
T5357 |
mark |
as,described |
| R3225 |
T5357 |
T5355 |
advcl |
described,obtained |
| R3226 |
T5358 |
T5357 |
advmod |
below,described |
| R3227 |
T5359 |
T5341 |
punct |
.,plated |
| R3228 |
T5361 |
T5362 |
det |
The,neurons |
| R3229 |
T5362 |
T5363 |
nsubjpass |
neurons,maintained |
| R3230 |
T5364 |
T5363 |
auxpass |
were,maintained |
| R3231 |
T5365 |
T5363 |
prep |
at,maintained |
| R3232 |
T5366 |
T5367 |
nummod |
37,°C |
| R3233 |
T5367 |
T5365 |
pobj |
°C,at |
| R3234 |
T5368 |
T5363 |
prep |
in,maintained |
| R3235 |
T5369 |
T5370 |
det |
a,atmosphere |
| R3236 |
T5370 |
T5368 |
pobj |
atmosphere,in |
| R3237 |
T5371 |
T5370 |
amod |
humidified,atmosphere |
| R3238 |
T5372 |
T5370 |
prep |
with,atmosphere |
| R3239 |
T5373 |
T5374 |
nummod |
5,% |
| R3240 |
T5374 |
T5375 |
compound |
%,CO2 |
| R3241 |
T5375 |
T5372 |
pobj |
CO2,with |
| R3242 |
T5376 |
T5363 |
punct |
.,maintained |
| R3243 |
T5378 |
T5379 |
amod |
Cortical,astrocytes |
| R3244 |
T5379 |
T5380 |
nsubjpass |
astrocytes,grown |
| R3245 |
T5381 |
T5379 |
acl |
dissociated,astrocytes |
| R3246 |
T5382 |
T5381 |
prep |
from,dissociated |
| R3247 |
T5383 |
T5384 |
amod |
newborn,mouse |
| R3248 |
T5384 |
T5385 |
compound |
mouse,cortices |
| R3249 |
T5385 |
T5382 |
pobj |
cortices,from |
| R3250 |
T5386 |
T5380 |
auxpass |
were,grown |
| R3251 |
T5387 |
T5380 |
prep |
in,grown |
| R3252 |
T5388 |
T5389 |
compound |
culture,flasks |
| R3253 |
T5389 |
T5387 |
pobj |
flasks,in |
| R3254 |
T5390 |
T5380 |
prep |
at,grown |
| R3255 |
T5391 |
T5392 |
nummod |
37,°C |
| R3256 |
T5392 |
T5390 |
pobj |
°C,at |
| R3257 |
T5393 |
T5380 |
prep |
in,grown |
| R3258 |
T5394 |
T5395 |
det |
a,atmosphere |
| R3259 |
T5395 |
T5393 |
pobj |
atmosphere,in |
| R3260 |
T5396 |
T5395 |
amod |
humidified,atmosphere |
| R3261 |
T5397 |
T5395 |
prep |
with,atmosphere |
| R3262 |
T5398 |
T5399 |
nummod |
5,% |
| R3263 |
T5399 |
T5400 |
compound |
%,CO2 |
| R3264 |
T5400 |
T5397 |
pobj |
CO2,with |
| R3265 |
T5401 |
T5380 |
prep |
until,grown |
| R3266 |
T5402 |
T5401 |
pcomp |
confluent,until |
| R3267 |
T5403 |
T5380 |
punct |
.,grown |
| R3268 |
T5405 |
T5406 |
det |
The,cells |
| R3269 |
T5406 |
T5407 |
nsubjpass |
cells,exposed |
| R3270 |
T5408 |
T5407 |
auxpass |
were,exposed |
| R3271 |
T5409 |
T5407 |
prep |
to,exposed |
| R3272 |
T5410 |
T5411 |
quantmod |
10,5 |
| R3273 |
T5411 |
T5413 |
nummod |
5,M |
| R3274 |
T5412 |
T5411 |
punct |
-,5 |
| R3275 |
T5413 |
T5414 |
compound |
M,arabinoside |
| R3276 |
T5414 |
T5409 |
pobj |
arabinoside,to |
| R3277 |
T5415 |
T5414 |
compound |
cytosine,arabinoside |
| R3278 |
T5416 |
T5417 |
punct |
(,Sigma |
| R3279 |
T5417 |
T5414 |
parataxis |
Sigma,arabinoside |
| R3280 |
T5418 |
T5417 |
punct |
),Sigma |
| R3281 |
T5419 |
T5407 |
cc |
and,exposed |
| R3282 |
T5420 |
T5407 |
conj |
cultured,exposed |
| R3283 |
T5421 |
T5420 |
prep |
for,cultured |
| R3284 |
T5422 |
T5423 |
amod |
additional,hrs |
| R3285 |
T5423 |
T5421 |
pobj |
hrs,for |
| R3286 |
T5424 |
T5425 |
quantmod |
12,24 |
| R3287 |
T5425 |
T5423 |
nummod |
24,hrs |
| R3288 |
T5426 |
T5425 |
punct |
–,24 |
| R3289 |
T5427 |
T5420 |
prep |
at,cultured |
| R3290 |
T5428 |
T5429 |
nummod |
37,°C |
| R3291 |
T5429 |
T5427 |
pobj |
°C,at |
| R3292 |
T5430 |
T5407 |
punct |
.,exposed |
| R3293 |
T5432 |
T5433 |
prep |
After,used |
| R3294 |
T5434 |
T5432 |
pobj |
remove,After |
| R3295 |
T5435 |
T5434 |
prep |
of,remove |
| R3296 |
T5436 |
T5437 |
det |
the,media |
| R3297 |
T5437 |
T5435 |
pobj |
media,of |
| R3298 |
T5438 |
T5434 |
prep |
with,remove |
| R3299 |
T5439 |
T5440 |
amod |
cellular,debris |
| R3300 |
T5440 |
T5438 |
pobj |
debris,with |
| R3301 |
T5441 |
T5433 |
punct |
", ",used |
| R3302 |
T5442 |
T5443 |
det |
the,cells |
| R3303 |
T5443 |
T5433 |
nsubjpass |
cells,used |
| R3304 |
T5444 |
T5433 |
auxpass |
were,used |
| R3305 |
T5445 |
T5433 |
prep |
for,used |
| R3306 |
T5446 |
T5445 |
pcomp |
coating,for |
| R3307 |
T5447 |
T5446 |
dobj |
coverslips,coating |
| R3308 |
T5448 |
T5433 |
punct |
.,used |
