Id |
Subject |
Object |
Predicate |
Lexical cue |
T4823 |
0-8 |
NN |
denotes |
Antibody |
T4824 |
9-19 |
NN |
denotes |
production |
T4825 |
20-23 |
CC |
denotes |
and |
T4826 |
24-31 |
NNP |
denotes |
Western |
T4827 |
32-36 |
NN |
denotes |
blot |
T4828 |
37-45 |
NNS |
denotes |
analyses |
T4829 |
45-271 |
sentence |
denotes |
Peptides linked to KLH (keyhole limpet hemacyanin) from Acdp1 N- and C-terminals and the conserved domain (ACD) were used for generation of antibodies specifically for Acdp1 and all Acdp members as reported, respectively [5]. |
T4830 |
46-54 |
NNS |
denotes |
Peptides |
T4832 |
55-61 |
VBN |
denotes |
linked |
T4833 |
62-64 |
IN |
denotes |
to |
T4834 |
65-68 |
NN |
denotes |
KLH |
T4835 |
69-70 |
-LRB- |
denotes |
( |
T4836 |
70-77 |
NN |
denotes |
keyhole |
T4838 |
78-84 |
NN |
denotes |
limpet |
T4837 |
85-95 |
NN |
denotes |
hemacyanin |
T4839 |
95-96 |
-RRB- |
denotes |
) |
T4840 |
97-101 |
IN |
denotes |
from |
T4841 |
102-107 |
NN |
denotes |
Acdp1 |
T4843 |
108-109 |
NN |
denotes |
N |
T4844 |
109-110 |
HYPH |
denotes |
- |
T4845 |
111-114 |
CC |
denotes |
and |
T4846 |
115-116 |
NN |
denotes |
C |
T4847 |
116-117 |
HYPH |
denotes |
- |
T4842 |
117-126 |
NNS |
denotes |
terminals |
T4848 |
127-130 |
CC |
denotes |
and |
T4849 |
131-134 |
DT |
denotes |
the |
T4851 |
135-144 |
VBN |
denotes |
conserved |
T4850 |
145-151 |
NN |
denotes |
domain |
T4852 |
152-153 |
-LRB- |
denotes |
( |
T4853 |
153-156 |
NN |
denotes |
ACD |
T4854 |
156-157 |
-RRB- |
denotes |
) |
T4855 |
158-162 |
VBD |
denotes |
were |
T4831 |
163-167 |
VBN |
denotes |
used |
T4856 |
168-171 |
IN |
denotes |
for |
T4857 |
172-182 |
NN |
denotes |
generation |
T4858 |
183-185 |
IN |
denotes |
of |
T4859 |
186-196 |
NNS |
denotes |
antibodies |
T4860 |
197-209 |
RB |
denotes |
specifically |
T4861 |
210-213 |
IN |
denotes |
for |
T4862 |
214-219 |
NN |
denotes |
Acdp1 |
T4863 |
220-223 |
CC |
denotes |
and |
T4864 |
224-227 |
DT |
denotes |
all |
T4866 |
228-232 |
NN |
denotes |
Acdp |
T4865 |
233-240 |
NNS |
denotes |
members |
T4867 |
241-243 |
IN |
denotes |
as |
T4868 |
244-252 |
VBN |
denotes |
reported |
T4869 |
252-254 |
, |
denotes |
, |
T4870 |
254-266 |
RB |
denotes |
respectively |
T4871 |
267-268 |
-LRB- |
denotes |
[ |
T4872 |
268-269 |
CD |
denotes |
5 |
T4873 |
269-270 |
-RRB- |
denotes |
] |
T4874 |
270-271 |
. |
denotes |
. |
T4875 |
271-350 |
sentence |
denotes |
Western blots were carried out Using ECL (PIERCE) as described previously [1]. |
T4876 |
272-279 |
NNP |
denotes |
Western |
T4877 |
280-285 |
NNS |
denotes |
blots |
T4879 |
286-290 |
VBD |
denotes |
were |
T4878 |
291-298 |
VBN |
denotes |
carried |
T4880 |
299-302 |
RP |
denotes |
out |
T4881 |
303-308 |
VBG |
denotes |
Using |
T4882 |
309-312 |
NN |
denotes |
ECL |
T4883 |
313-314 |
-LRB- |
denotes |
( |
T4884 |
314-320 |
NN |
denotes |
PIERCE |
T4885 |
320-321 |
-RRB- |
denotes |
) |
T4886 |
322-324 |
IN |
denotes |
as |
T4887 |
325-334 |
VBN |
denotes |
described |
T4888 |
335-345 |
RB |
denotes |
previously |
T4889 |
346-347 |
-LRB- |
denotes |
[ |
T4890 |
347-348 |
CD |
denotes |
1 |
T4891 |
348-349 |
-RRB- |
denotes |
] |
T4892 |
349-350 |
. |
denotes |
. |
T4893 |
350-512 |
sentence |
denotes |
The membranes were washed extensively after incubation with primary and secondary antibodies and were then developed with X-ray films with optimal exposure time. |
T4894 |
351-354 |
DT |
denotes |
The |
T4895 |
355-364 |
NNS |
denotes |
membranes |
T4897 |
365-369 |
VBD |
denotes |
were |
T4896 |
370-376 |
VBN |
denotes |
washed |
T4898 |
377-388 |
RB |
denotes |
extensively |
T4899 |
389-394 |
IN |
denotes |
after |
T4900 |
395-405 |
NN |
denotes |
incubation |
T4901 |
406-410 |
IN |
denotes |
with |
T4902 |
411-418 |
JJ |
denotes |
primary |
T4904 |
419-422 |
CC |
denotes |
and |
T4905 |
423-432 |
JJ |
denotes |
secondary |
T4903 |
433-443 |
NNS |
denotes |
antibodies |
T4906 |
444-447 |
CC |
denotes |
and |
T4907 |
448-452 |
VBD |
denotes |
were |
T4909 |
453-457 |
RB |
denotes |
then |
T4908 |
458-467 |
VBN |
denotes |
developed |
T4910 |
468-472 |
IN |
denotes |
with |
T4911 |
473-478 |
NN |
denotes |
X-ray |
T4912 |
479-484 |
NNS |
denotes |
films |
T4913 |
485-489 |
IN |
denotes |
with |
T4914 |
490-497 |
JJ |
denotes |
optimal |
T4916 |
498-506 |
NN |
denotes |
exposure |
T4915 |
507-511 |
NN |
denotes |
time |
T4917 |
511-512 |
. |
denotes |
. |
R2904 |
T4823 |
T4824 |
compound |
Antibody,production |
R2905 |
T4825 |
T4824 |
cc |
and,production |
R2906 |
T4826 |
T4827 |
compound |
Western,blot |
R2907 |
T4827 |
T4828 |
compound |
blot,analyses |
R2908 |
T4828 |
T4824 |
conj |
analyses,production |
R2909 |
T4830 |
T4831 |
nsubjpass |
Peptides,used |
R2910 |
T4832 |
T4830 |
acl |
linked,Peptides |
R2911 |
T4833 |
T4832 |
prep |
to,linked |
R2912 |
T4834 |
T4833 |
pobj |
KLH,to |
R2913 |
T4835 |
T4834 |
punct |
(,KLH |
R2914 |
T4836 |
T4837 |
compound |
keyhole,hemacyanin |
R2915 |
T4837 |
T4834 |
appos |
hemacyanin,KLH |
R2916 |
T4838 |
T4837 |
compound |
limpet,hemacyanin |
R2917 |
T4839 |
T4833 |
punct |
),to |
R2918 |
T4840 |
T4833 |
prep |
from,to |
R2919 |
T4841 |
T4842 |
nmod |
Acdp1,terminals |
R2920 |
T4842 |
T4840 |
pobj |
terminals,from |
R2921 |
T4843 |
T4842 |
nmod |
N,terminals |
R2922 |
T4844 |
T4843 |
punct |
-,N |
R2923 |
T4845 |
T4843 |
cc |
and,N |
R2924 |
T4846 |
T4843 |
conj |
C,N |
R2925 |
T4847 |
T4842 |
punct |
-,terminals |
R2926 |
T4848 |
T4842 |
cc |
and,terminals |
R2927 |
T4849 |
T4850 |
det |
the,domain |
R2928 |
T4850 |
T4842 |
conj |
domain,terminals |
R2929 |
T4851 |
T4850 |
amod |
conserved,domain |
R2930 |
T4852 |
T4850 |
punct |
(,domain |
R2931 |
T4853 |
T4850 |
appos |
ACD,domain |
R2932 |
T4854 |
T4831 |
punct |
),used |
R2933 |
T4855 |
T4831 |
auxpass |
were,used |
R2934 |
T4856 |
T4831 |
prep |
for,used |
R2935 |
T4857 |
T4856 |
pobj |
generation,for |
R2936 |
T4858 |
T4857 |
prep |
of,generation |
R2937 |
T4859 |
T4858 |
pobj |
antibodies,of |
R2938 |
T4860 |
T4861 |
advmod |
specifically,for |
R2939 |
T4861 |
T4856 |
prep |
for,for |
R2940 |
T4862 |
T4861 |
pobj |
Acdp1,for |
R2941 |
T4863 |
T4862 |
cc |
and,Acdp1 |
R2942 |
T4864 |
T4865 |
det |
all,members |
R2943 |
T4865 |
T4862 |
conj |
members,Acdp1 |
R2944 |
T4866 |
T4865 |
compound |
Acdp,members |
R2945 |
T4867 |
T4868 |
mark |
as,reported |
R2946 |
T4868 |
T4831 |
advcl |
reported,used |
R2947 |
T4869 |
T4831 |
punct |
", ",used |
R2948 |
T4870 |
T4831 |
advmod |
respectively,used |
R2949 |
T4871 |
T4872 |
punct |
[,5 |
R2950 |
T4872 |
T4831 |
parataxis |
5,used |
R2951 |
T4873 |
T4872 |
punct |
],5 |
R2952 |
T4874 |
T4831 |
punct |
.,used |
R2953 |
T4876 |
T4877 |
compound |
Western,blots |
R2954 |
T4877 |
T4878 |
nsubjpass |
blots,carried |
R2955 |
T4879 |
T4878 |
auxpass |
were,carried |
R2956 |
T4880 |
T4878 |
prt |
out,carried |
R2957 |
T4881 |
T4878 |
advcl |
Using,carried |
R2958 |
T4882 |
T4881 |
dobj |
ECL,Using |
R2959 |
T4883 |
T4884 |
punct |
(,PIERCE |
R2960 |
T4884 |
T4882 |
parataxis |
PIERCE,ECL |
R2961 |
T4885 |
T4884 |
punct |
),PIERCE |
R2962 |
T4886 |
T4887 |
mark |
as,described |
R2963 |
T4887 |
T4878 |
advcl |
described,carried |
R2964 |
T4888 |
T4887 |
advmod |
previously,described |
R2965 |
T4889 |
T4890 |
punct |
[,1 |
R2966 |
T4890 |
T4878 |
parataxis |
1,carried |
R2967 |
T4891 |
T4890 |
punct |
],1 |
R2968 |
T4892 |
T4878 |
punct |
.,carried |
R2969 |
T4894 |
T4895 |
det |
The,membranes |
R2970 |
T4895 |
T4896 |
nsubjpass |
membranes,washed |
R2971 |
T4897 |
T4896 |
auxpass |
were,washed |
R2972 |
T4898 |
T4896 |
advmod |
extensively,washed |
R2973 |
T4899 |
T4896 |
prep |
after,washed |
R2974 |
T4900 |
T4899 |
pobj |
incubation,after |
R2975 |
T4901 |
T4900 |
prep |
with,incubation |
R2976 |
T4902 |
T4903 |
amod |
primary,antibodies |
R2977 |
T4903 |
T4901 |
pobj |
antibodies,with |
R2978 |
T4904 |
T4902 |
cc |
and,primary |
R2979 |
T4905 |
T4902 |
conj |
secondary,primary |
R2980 |
T4906 |
T4896 |
cc |
and,washed |
R2981 |
T4907 |
T4908 |
auxpass |
were,developed |
R2982 |
T4908 |
T4896 |
conj |
developed,washed |
R2983 |
T4909 |
T4908 |
advmod |
then,developed |
R2984 |
T4910 |
T4908 |
prep |
with,developed |
R2985 |
T4911 |
T4912 |
compound |
X-ray,films |
R2986 |
T4912 |
T4910 |
pobj |
films,with |
R2987 |
T4913 |
T4912 |
prep |
with,films |
R2988 |
T4914 |
T4915 |
amod |
optimal,time |
R2989 |
T4915 |
T4913 |
pobj |
time,with |
R2990 |
T4916 |
T4915 |
compound |
exposure,time |
R2991 |
T4917 |
T4896 |
punct |
.,washed |