PMC:340383 / 1718-23691 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T593	11-13	PRP	denotes	We
T595	14-18	VBP	denotes	have
T596	19-27	RB	denotes	recently
T594	28-34	VBN	denotes	cloned
T597	35-38	CC	denotes	and
T598	39-52	VBN	denotes	characterized
T599	53-54	DT	denotes	a
T601	55-60	JJ	denotes	novel
T602	61-65	NN	denotes	gene
T600	66-72	NN	denotes	family
T603	73-78	VBN	denotes	named
T604	79-86	JJ	denotes	ancient
T606	87-96	VBN	denotes	conserved
T607	97-103	NN	denotes	domain
T605	104-111	NN	denotes	protein
T608	112-113	-LRB-	denotes	(
T609	113-117	NN	denotes	ACDP
T610	117-118	-RRB-	denotes	)
T611	119-124	WDT	denotes	which
T612	125-132	VBZ	denotes	encodes
T613	133-137	CD	denotes	four
T615	138-145	NN	denotes	protein
T614	146-153	NNS	denotes	members
T616	154-156	IN	denotes	in
T617	157-163	NNS	denotes	humans
T618	164-165	-LRB-	denotes	[
T619	165-166	CD	denotes	1
T620	166-167	-RRB-	denotes	]
T621	167-168	.	denotes	.
T622	168-320	sentence	denotes	We found that this gene family is evolutionarily conserved in diverse species ranging from bacteria, yeast, C. elegans, and D. melanogaster to mammals.
T623	169-171	PRP	denotes	We
T624	172-177	VBD	denotes	found
T625	178-182	IN	denotes	that
T627	183-187	DT	denotes	this
T629	188-192	NN	denotes	gene
T628	193-199	NN	denotes	family
T626	200-202	VBZ	denotes	is
T630	203-217	RB	denotes	evolutionarily
T631	218-227	JJ	denotes	conserved
T632	228-230	IN	denotes	in
T633	231-238	JJ	denotes	diverse
T634	239-246	NNS	denotes	species
T635	247-254	VBG	denotes	ranging
T636	255-259	IN	denotes	from
T637	260-268	NNS	denotes	bacteria
T638	268-270	,	denotes	,
T639	270-275	NN	denotes	yeast
T640	275-277	,	denotes	,
T641	277-279	NNP	denotes	C.
T642	280-287	NNP	denotes	elegans
T643	287-289	,	denotes	,
T644	289-292	CC	denotes	and
T645	293-295	NNP	denotes	D.
T646	296-308	NNP	denotes	melanogaster
T647	309-311	IN	denotes	to
T648	312-319	NNS	denotes	mammals
T649	319-320	.	denotes	.
T650	320-459	sentence	denotes	The sequence conservation and the presence of multiple members within a species may imply functional importance associated with the genes.
T651	321-324	DT	denotes	The
T653	325-333	NN	denotes	sequence
T652	334-346	NN	denotes	conservation
T655	347-350	CC	denotes	and
T656	351-354	DT	denotes	the
T657	355-363	NN	denotes	presence
T658	364-366	IN	denotes	of
T659	367-375	JJ	denotes	multiple
T660	376-383	NNS	denotes	members
T661	384-390	IN	denotes	within
T662	391-392	DT	denotes	a
T663	393-400	NN	denotes	species
T664	401-404	MD	denotes	may
T654	405-410	VB	denotes	imply
T665	411-421	JJ	denotes	functional
T666	422-432	NN	denotes	importance
T667	433-443	VBN	denotes	associated
T668	444-448	IN	denotes	with
T669	449-452	DT	denotes	the
T670	453-458	NNS	denotes	genes
T671	458-459	.	denotes	.
T672	459-607	sentence	denotes	To facilitate the functional analysis of the ACDP gene family, we cloned and characterized Acdp, the mouse homologue of the human ACDP gene family.
T673	460-462	TO	denotes	To
T674	463-473	VB	denotes	facilitate
T676	474-477	DT	denotes	the
T678	478-488	JJ	denotes	functional
T677	489-497	NN	denotes	analysis
T679	498-500	IN	denotes	of
T680	501-504	DT	denotes	the
T682	505-509	NN	denotes	ACDP
T683	510-514	NN	denotes	gene
T681	515-521	NN	denotes	family
T684	521-523	,	denotes	,
T685	523-525	PRP	denotes	we
T675	526-532	VBD	denotes	cloned
T686	533-536	CC	denotes	and
T687	537-550	VBD	denotes	characterized
T688	551-555	NN	denotes	Acdp
T689	555-557	,	denotes	,
T690	557-560	DT	denotes	the
T692	561-566	NN	denotes	mouse
T691	567-576	NN	denotes	homologue
T693	577-579	IN	denotes	of
T694	580-583	DT	denotes	the
T696	584-589	JJ	denotes	human
T697	590-594	NN	denotes	ACDP
T698	595-599	NN	denotes	gene
T695	600-606	NN	denotes	family
T699	606-607	.	denotes	.
T863	618-627	JJ	denotes	Molecular
T864	628-635	NN	denotes	cloning
T865	636-638	IN	denotes	of
T866	639-642	DT	denotes	the
T868	643-647	NN	denotes	Acdp
T869	648-652	NN	denotes	gene
T867	653-659	NN	denotes	family
T870	659-823	sentence	denotes	To clone the mouse Acdp genes, the human ACDP cDNA and predicted protein sequences were used to search the mouse EST database with the blastn and tblastn programs.
T871	660-662	TO	denotes	To
T872	663-668	VB	denotes	clone
T874	669-672	DT	denotes	the
T876	673-678	NN	denotes	mouse
T877	679-683	NN	denotes	Acdp
T875	684-689	NNS	denotes	genes
T878	689-691	,	denotes	,
T879	691-694	DT	denotes	the
T881	695-700	JJ	denotes	human
T882	701-705	NN	denotes	ACDP
T880	706-710	NN	denotes	cDNA
T883	711-714	CC	denotes	and
T884	715-724	JJ	denotes	predicted
T886	725-732	NN	denotes	protein
T885	733-742	NNS	denotes	sequences
T887	743-747	VBD	denotes	were
T873	748-752	VBN	denotes	used
T888	753-755	TO	denotes	to
T889	756-762	VB	denotes	search
T890	763-766	DT	denotes	the
T892	767-772	NN	denotes	mouse
T893	773-776	NN	denotes	EST
T891	777-785	NN	denotes	database
T894	786-790	IN	denotes	with
T895	791-794	DT	denotes	the
T897	795-801	NN	denotes	blastn
T898	802-805	CC	denotes	and
T899	806-813	NN	denotes	tblastn
T896	814-822	NNS	denotes	programs
T900	822-823	.	denotes	.
T901	823-897	sentence	denotes	Mouse EST markers corresponding to each Acdp member were then identified.
T902	824-829	NN	denotes	Mouse
T904	830-833	NN	denotes	EST
T903	834-841	NNS	denotes	markers
T906	842-855	VBG	denotes	corresponding
T907	856-858	IN	denotes	to
T908	859-863	DT	denotes	each
T910	864-868	NN	denotes	Acdp
T909	869-875	NN	denotes	member
T911	876-880	VBD	denotes	were
T912	881-885	RB	denotes	then
T905	886-896	VBN	denotes	identified
T913	896-897	.	denotes	.
T914	897-1011	sentence	denotes	For example, EST H3086H12-5 corresponds to Acdp1, W98010 for Acdp2, 603299135F1 for Acdp3 and BG083791 for Acdp4.
T915	898-901	IN	denotes	For
T917	902-909	NN	denotes	example
T918	909-911	,	denotes	,
T919	911-914	NN	denotes	EST
T920	915-923	NN	denotes	H3086H12
T921	923-924	HYPH	denotes	-
T922	924-925	CD	denotes	5
T916	926-937	VBZ	denotes	corresponds
T923	938-940	IN	denotes	to
T924	941-946	NN	denotes	Acdp1
T925	946-948	,	denotes	,
T926	948-954	NN	denotes	W98010
T927	955-958	IN	denotes	for
T928	959-964	NN	denotes	Acdp2
T929	964-966	,	denotes	,
T930	966-977	NN	denotes	603299135F1
T931	978-981	IN	denotes	for
T932	982-987	NN	denotes	Acdp3
T933	988-991	CC	denotes	and
T934	992-1000	NN	denotes	BG083791
T935	1001-1004	IN	denotes	for
T936	1005-1010	NN	denotes	Acdp4
T937	1010-1011	.	denotes	.
T938	1011-1077	sentence	denotes	A modified oligo-dT with a M13 tail was used for the RT reaction.
T939	1012-1013	DT	denotes	A
T941	1014-1022	VBN	denotes	modified
T942	1023-1028	NN	denotes	oligo
T943	1028-1029	HYPH	denotes	-
T940	1029-1031	NN	denotes	dT
T945	1032-1036	IN	denotes	with
T946	1037-1038	DT	denotes	a
T948	1039-1042	NN	denotes	M13
T947	1043-1047	NN	denotes	tail
T949	1048-1051	VBD	denotes	was
T944	1052-1056	VBN	denotes	used
T950	1057-1060	IN	denotes	for
T951	1061-1064	DT	denotes	the
T953	1065-1067	NN	denotes	RT
T952	1068-1076	NN	denotes	reaction
T954	1076-1077	.	denotes	.
T955	1077-1245	sentence	denotes	A forward primer from each EST marker and the M13 primer (olig-dT tail) were used to amplify the 3' UTR sequence for each corresponding Acdp gene from the RT products.
T956	1078-1079	DT	denotes	A
T958	1080-1087	JJ	denotes	forward
T957	1088-1094	NN	denotes	primer
T960	1095-1099	IN	denotes	from
T961	1100-1104	DT	denotes	each
T963	1105-1108	NN	denotes	EST
T962	1109-1115	NN	denotes	marker
T964	1116-1119	CC	denotes	and
T965	1120-1123	DT	denotes	the
T967	1124-1127	NN	denotes	M13
T966	1128-1134	NN	denotes	primer
T968	1135-1136	-LRB-	denotes	(
T969	1136-1140	NN	denotes	olig
T971	1140-1141	HYPH	denotes	-
T970	1141-1143	NN	denotes	dT
T972	1144-1148	NN	denotes	tail
T973	1148-1149	-RRB-	denotes	)
T974	1150-1154	VBD	denotes	were
T959	1155-1159	VBN	denotes	used
T975	1160-1162	TO	denotes	to
T976	1163-1170	VB	denotes	amplify
T977	1171-1174	DT	denotes	the
T979	1175-1176	CD	denotes	3
T981	1176-1177	SYM	denotes	'
T980	1178-1181	NN	denotes	UTR
T978	1182-1190	NN	denotes	sequence
T982	1191-1194	IN	denotes	for
T983	1195-1199	DT	denotes	each
T985	1200-1213	VBG	denotes	corresponding
T986	1214-1218	NN	denotes	Acdp
T984	1219-1223	NN	denotes	gene
T987	1224-1228	IN	denotes	from
T988	1229-1232	DT	denotes	the
T990	1233-1235	NN	denotes	RT
T989	1236-1244	NNS	denotes	products
T991	1244-1245	.	denotes	.
T992	1245-1378	sentence	denotes	To obtain 5'-end coding sequences for the Acdp genes, we conducted a series nested PCR with combinations of mouse and human primers.
T993	1246-1248	TO	denotes	To
T994	1249-1255	VB	denotes	obtain
T996	1256-1257	CD	denotes	5
T998	1257-1258	SYM	denotes	'
T999	1258-1259	HYPH	denotes	-
T997	1259-1262	NN	denotes	end
T1001	1263-1269	VBG	denotes	coding
T1000	1270-1279	NNS	denotes	sequences
T1002	1280-1283	IN	denotes	for
T1003	1284-1287	DT	denotes	the
T1005	1288-1292	NN	denotes	Acdp
T1004	1293-1298	NNS	denotes	genes
T1006	1298-1300	,	denotes	,
T1007	1300-1302	PRP	denotes	we
T995	1303-1312	VBD	denotes	conducted
T1008	1313-1314	DT	denotes	a
T1010	1315-1321	NN	denotes	series
T1011	1322-1328	VBN	denotes	nested
T1009	1329-1332	NN	denotes	PCR
T1012	1333-1337	IN	denotes	with
T1013	1338-1350	NNS	denotes	combinations
T1014	1351-1353	IN	denotes	of
T1015	1354-1359	NN	denotes	mouse
T1017	1360-1363	CC	denotes	and
T1018	1364-1369	JJ	denotes	human
T1016	1370-1377	NNS	denotes	primers
T1019	1377-1378	.	denotes	.
T1020	1378-1487	sentence	denotes	The 5' UTR sequences were identified by directly sequencing BAC DNA containing the corresponding Acdp genes.
T1021	1379-1382	DT	denotes	The
T1023	1383-1384	CD	denotes	5
T1024	1384-1385	SYM	denotes	'
T1025	1386-1389	NN	denotes	UTR
T1022	1390-1399	NNS	denotes	sequences
T1027	1400-1404	VBD	denotes	were
T1026	1405-1415	VBN	denotes	identified
T1028	1416-1418	IN	denotes	by
T1029	1419-1427	RB	denotes	directly
T1030	1428-1438	VBG	denotes	sequencing
T1031	1439-1442	NN	denotes	BAC
T1032	1443-1446	NN	denotes	DNA
T1033	1447-1457	VBG	denotes	containing
T1034	1458-1461	DT	denotes	the
T1036	1462-1475	VBG	denotes	corresponding
T1037	1476-1480	NN	denotes	Acdp
T1035	1481-1486	NNS	denotes	genes
T1038	1486-1487	.	denotes	.
T1039	1487-1581	sentence	denotes	The BAC clones were identified by screening a CITB mouse BAC DNA library (Research Genetics).
T1040	1488-1491	DT	denotes	The
T1042	1492-1495	NN	denotes	BAC
T1041	1496-1502	NNS	denotes	clones
T1044	1503-1507	VBD	denotes	were
T1043	1508-1518	VBN	denotes	identified
T1045	1519-1521	IN	denotes	by
T1046	1522-1531	VBG	denotes	screening
T1047	1532-1533	DT	denotes	a
T1049	1534-1538	NN	denotes	CITB
T1051	1539-1544	NN	denotes	mouse
T1052	1545-1548	NN	denotes	BAC
T1050	1549-1552	NN	denotes	DNA
T1048	1553-1560	NN	denotes	library
T1053	1561-1562	-LRB-	denotes	(
T1055	1562-1570	NNP	denotes	Research
T1054	1571-1579	NNP	denotes	Genetics
T1056	1579-1580	-RRB-	denotes	)
T1057	1580-1581	.	denotes	.
T1058	1581-1656	sentence	denotes	The 5' UTR sequences obtained from above were further confirmed by RT-PCR.
T1059	1582-1585	DT	denotes	The
T1061	1586-1587	CD	denotes	5
T1062	1587-1588	SYM	denotes	'
T1063	1589-1592	NN	denotes	UTR
T1060	1593-1602	NNS	denotes	sequences
T1065	1603-1611	VBN	denotes	obtained
T1066	1612-1616	IN	denotes	from
T1067	1617-1622	RB	denotes	above
T1068	1623-1627	VBD	denotes	were
T1069	1628-1635	RB	denotes	further
T1064	1636-1645	VBN	denotes	confirmed
T1070	1646-1648	IN	denotes	by
T1071	1649-1651	NN	denotes	RT
T1073	1651-1652	HYPH	denotes	-
T1072	1652-1655	NN	denotes	PCR
T1074	1655-1656	.	denotes	.
T1075	1656-1771	sentence	denotes	The Acdp1 gene contains 3,631 bp of nucleotide sequence and encodes a predicted protein with 951 amino acids (AA).
T1076	1657-1660	DT	denotes	The
T1078	1661-1666	NN	denotes	Acdp1
T1077	1667-1671	NN	denotes	gene
T1079	1672-1680	VBZ	denotes	contains
T1080	1681-1686	CD	denotes	3,631
T1081	1687-1689	NNS	denotes	bp
T1082	1690-1692	IN	denotes	of
T1083	1693-1703	NN	denotes	nucleotide
T1084	1704-1712	NN	denotes	sequence
T1085	1713-1716	CC	denotes	and
T1086	1717-1724	VBZ	denotes	encodes
T1087	1725-1726	DT	denotes	a
T1089	1727-1736	VBN	denotes	predicted
T1088	1737-1744	NN	denotes	protein
T1090	1745-1749	IN	denotes	with
T1091	1750-1753	CD	denotes	951
T1093	1754-1759	NN	denotes	amino
T1092	1760-1765	NNS	denotes	acids
T1094	1766-1767	-LRB-	denotes	(
T1095	1767-1769	NN	denotes	AA
T1096	1769-1770	-RRB-	denotes	)
T1097	1770-1771	.	denotes	.
T1098	1771-1973	sentence	denotes	The other three Acdp genes (Acdp2, 3 and 4) contain 3,244 bp, 2,684 bp and 2,743 bp of cDNA sequences, and encode deduced proteins of 874 amino acids, 713 amino acids and 771 amino acids, respectively.
T1099	1772-1775	DT	denotes	The
T1101	1776-1781	JJ	denotes	other
T1102	1782-1787	CD	denotes	three
T1103	1788-1792	NN	denotes	Acdp
T1100	1793-1798	NNS	denotes	genes
T1105	1799-1800	-LRB-	denotes	(
T1106	1800-1805	NN	denotes	Acdp2
T1107	1805-1807	,	denotes	,
T1108	1807-1808	CD	denotes	3
T1109	1809-1812	CC	denotes	and
T1110	1813-1814	CD	denotes	4
T1111	1814-1815	-RRB-	denotes	)
T1104	1816-1823	VBP	denotes	contain
T1112	1824-1829	CD	denotes	3,244
T1113	1830-1832	NNS	denotes	bp
T1114	1832-1834	,	denotes	,
T1115	1834-1839	CD	denotes	2,684
T1116	1840-1842	NNS	denotes	bp
T1117	1843-1846	CC	denotes	and
T1118	1847-1852	CD	denotes	2,743
T1119	1853-1855	NNS	denotes	bp
T1120	1856-1858	IN	denotes	of
T1121	1859-1863	NN	denotes	cDNA
T1122	1864-1873	NNS	denotes	sequences
T1123	1873-1875	,	denotes	,
T1124	1875-1878	CC	denotes	and
T1125	1879-1885	VBP	denotes	encode
T1126	1886-1893	VBN	denotes	deduced
T1127	1894-1902	NN	denotes	proteins
T1128	1903-1905	IN	denotes	of
T1129	1906-1909	CD	denotes	874
T1131	1910-1915	NN	denotes	amino
T1130	1916-1921	NNS	denotes	acids
T1132	1921-1923	,	denotes	,
T1133	1923-1926	CD	denotes	713
T1135	1927-1932	NN	denotes	amino
T1134	1933-1938	NNS	denotes	acids
T1136	1939-1942	CC	denotes	and
T1137	1943-1946	CD	denotes	771
T1139	1947-1952	NN	denotes	amino
T1138	1953-1958	NNS	denotes	acids
T1140	1958-1960	,	denotes	,
T1141	1960-1972	RB	denotes	respectively
T1142	1972-1973	.	denotes	.
T1296	1975-1981	NN	denotes	Tissue
T1297	1982-1994	NN	denotes	distribution
T1298	1994-2098	sentence	denotes	Northern blot analyses of the Acdp gene family were carried out using membranes purchased from Origene.
T1299	1995-2003	NNP	denotes	Northern
T1300	2004-2008	NN	denotes	blot
T1301	2009-2017	NNS	denotes	analyses
T1303	2018-2020	IN	denotes	of
T1304	2021-2024	DT	denotes	the
T1306	2025-2029	NN	denotes	Acdp
T1307	2030-2034	NN	denotes	gene
T1305	2035-2041	NN	denotes	family
T1308	2042-2046	VBD	denotes	were
T1302	2047-2054	VBN	denotes	carried
T1309	2055-2058	RP	denotes	out
T1310	2059-2064	VBG	denotes	using
T1311	2065-2074	NNS	denotes	membranes
T1312	2075-2084	VBN	denotes	purchased
T1313	2085-2089	IN	denotes	from
T1314	2090-2097	NNP	denotes	Origene
T1315	2097-2098	.	denotes	.
T1316	2098-2163	sentence	denotes	A total of 12 mouse tissues were included in the study (Fig. 1).
T1317	2099-2100	DT	denotes	A
T1318	2101-2106	NN	denotes	total
T1320	2107-2109	IN	denotes	of
T1321	2110-2112	CD	denotes	12
T1323	2113-2118	NN	denotes	mouse
T1322	2119-2126	NNS	denotes	tissues
T1324	2127-2131	VBD	denotes	were
T1319	2132-2140	VBN	denotes	included
T1325	2141-2143	IN	denotes	in
T1326	2144-2147	DT	denotes	the
T1327	2148-2153	NN	denotes	study
T1328	2154-2155	-LRB-	denotes	(
T1329	2155-2159	NN	denotes	Fig.
T1330	2160-2161	CD	denotes	1
T1331	2161-2162	-RRB-	denotes	)
T1332	2162-2163	.	denotes	.
T1333	2163-2350	sentence	denotes	Due to sequence homologies between each Acdp member within the conserved domain, probes for Northern bolts were PCR fragments from the last exon and the 3' untranslated region sequences.
T1334	2164-2167	IN	denotes	Due
T1336	2168-2170	IN	denotes	to
T1337	2171-2179	NN	denotes	sequence
T1338	2180-2190	NNS	denotes	homologies
T1339	2191-2198	IN	denotes	between
T1340	2199-2203	DT	denotes	each
T1342	2204-2208	NN	denotes	Acdp
T1341	2209-2215	NN	denotes	member
T1343	2216-2222	IN	denotes	within
T1344	2223-2226	DT	denotes	the
T1346	2227-2236	VBN	denotes	conserved
T1345	2237-2243	NN	denotes	domain
T1347	2243-2245	,	denotes	,
T1348	2245-2251	NNS	denotes	probes
T1349	2252-2255	IN	denotes	for
T1350	2256-2264	NNP	denotes	Northern
T1351	2265-2270	NNS	denotes	bolts
T1335	2271-2275	VBD	denotes	were
T1352	2276-2279	NN	denotes	PCR
T1353	2280-2289	NNS	denotes	fragments
T1354	2290-2294	IN	denotes	from
T1355	2295-2298	DT	denotes	the
T1357	2299-2303	JJ	denotes	last
T1356	2304-2308	NN	denotes	exon
T1358	2309-2312	CC	denotes	and
T1359	2313-2316	DT	denotes	the
T1361	2317-2318	CD	denotes	3
T1363	2318-2319	SYM	denotes	'
T1364	2320-2332	JJ	denotes	untranslated
T1362	2333-2339	NN	denotes	region
T1360	2340-2349	NNS	denotes	sequences
T1365	2349-2350	.	denotes	.
T1366	2350-2443	sentence	denotes	The mouse Acdp messages showed almost the same tissue distributions as the human ACDP genes.
T1367	2351-2354	DT	denotes	The
T1369	2355-2360	NN	denotes	mouse
T1370	2361-2365	NN	denotes	Acdp
T1368	2366-2374	NNS	denotes	messages
T1371	2375-2381	VBD	denotes	showed
T1372	2382-2388	RB	denotes	almost
T1374	2389-2392	DT	denotes	the
T1375	2393-2397	JJ	denotes	same
T1376	2398-2404	NN	denotes	tissue
T1373	2405-2418	NNS	denotes	distributions
T1377	2419-2421	IN	denotes	as
T1378	2422-2425	DT	denotes	the
T1380	2426-2431	JJ	denotes	human
T1381	2432-2436	NN	denotes	ACDP
T1379	2437-2442	NNS	denotes	genes
T1382	2442-2443	.	denotes	.
T1383	2443-2553	sentence	denotes	Acdp1 message is highly expressed in the brain, while kidney and testis also showed low levels of expression.
T1384	2444-2449	NN	denotes	Acdp1
T1385	2450-2457	NN	denotes	message
T1387	2458-2460	VBZ	denotes	is
T1388	2461-2467	RB	denotes	highly
T1386	2468-2477	VBN	denotes	expressed
T1389	2478-2480	IN	denotes	in
T1390	2481-2484	DT	denotes	the
T1391	2485-2490	NN	denotes	brain
T1392	2490-2492	,	denotes	,
T1393	2492-2497	IN	denotes	while
T1395	2498-2504	NN	denotes	kidney
T1396	2505-2508	CC	denotes	and
T1397	2509-2515	NN	denotes	testis
T1398	2516-2520	RB	denotes	also
T1394	2521-2527	VBD	denotes	showed
T1399	2528-2531	JJ	denotes	low
T1400	2532-2538	NNS	denotes	levels
T1401	2539-2541	IN	denotes	of
T1402	2542-2552	NN	denotes	expression
T1403	2552-2553	.	denotes	.
T1404	2553-2617	sentence	denotes	Acdp2 showed higher expressions in the brain, kidney and liver.
T1405	2554-2559	NN	denotes	Acdp2
T1406	2560-2566	VBD	denotes	showed
T1407	2567-2573	JJR	denotes	higher
T1408	2574-2585	NNS	denotes	expressions
T1409	2586-2588	IN	denotes	in
T1410	2589-2592	DT	denotes	the
T1411	2593-2598	NN	denotes	brain
T1412	2598-2600	,	denotes	,
T1413	2600-2606	NN	denotes	kidney
T1414	2607-2610	CC	denotes	and
T1415	2611-2616	NN	denotes	liver
T1416	2616-2617	.	denotes	.
T1417	2617-2764	sentence	denotes	However, the Acdp2 transcript was not present in the skeleton muscle and skin, and it showed very low levels of expression in the rest of tissues.
T1418	2618-2625	RB	denotes	However
T1420	2625-2627	,	denotes	,
T1421	2627-2630	DT	denotes	the
T1423	2631-2636	NN	denotes	Acdp2
T1422	2637-2647	NN	denotes	transcript
T1419	2648-2651	VBD	denotes	was
T1424	2652-2655	RB	denotes	not
T1425	2656-2663	JJ	denotes	present
T1426	2664-2666	IN	denotes	in
T1427	2667-2670	DT	denotes	the
T1429	2671-2679	NN	denotes	skeleton
T1428	2680-2686	NN	denotes	muscle
T1430	2687-2690	CC	denotes	and
T1431	2691-2695	NN	denotes	skin
T1432	2695-2697	,	denotes	,
T1433	2697-2700	CC	denotes	and
T1434	2701-2703	PRP	denotes	it
T1435	2704-2710	VBD	denotes	showed
T1436	2711-2715	RB	denotes	very
T1437	2716-2719	JJ	denotes	low
T1438	2720-2726	NNS	denotes	levels
T1439	2727-2729	IN	denotes	of
T1440	2730-2740	NN	denotes	expression
T1441	2741-2743	IN	denotes	in
T1442	2744-2747	DT	denotes	the
T1443	2748-2752	NN	denotes	rest
T1444	2753-2755	IN	denotes	of
T1445	2756-2763	NNS	denotes	tissues
T1446	2763-2764	.	denotes	.
T1447	2764-3023	sentence	denotes	Acdp3 and Acdp4 showed different levels of expression in all tissues tested; the highest expressions for Acdp3 were observed in the brain, kidney, liver and heart, and the highest expressions for Acdp4 were observed in the kidney, small intestine and testis.
T1448	2765-2770	NN	denotes	Acdp3
T1450	2771-2774	CC	denotes	and
T1451	2775-2780	NN	denotes	Acdp4
T1449	2781-2787	VBD	denotes	showed
T1453	2788-2797	JJ	denotes	different
T1454	2798-2804	NNS	denotes	levels
T1455	2805-2807	IN	denotes	of
T1456	2808-2818	NN	denotes	expression
T1457	2819-2821	IN	denotes	in
T1458	2822-2825	DT	denotes	all
T1459	2826-2833	NNS	denotes	tissues
T1460	2834-2840	VBN	denotes	tested
T1461	2840-2841	:	denotes	;
T1462	2842-2845	DT	denotes	the
T1464	2846-2853	JJS	denotes	highest
T1463	2854-2865	NNS	denotes	expressions
T1465	2866-2869	IN	denotes	for
T1466	2870-2875	NN	denotes	Acdp3
T1467	2876-2880	VBD	denotes	were
T1452	2881-2889	VBN	denotes	observed
T1468	2890-2892	IN	denotes	in
T1469	2893-2896	DT	denotes	the
T1470	2897-2902	NN	denotes	brain
T1471	2902-2904	,	denotes	,
T1472	2904-2910	NN	denotes	kidney
T1473	2910-2912	,	denotes	,
T1474	2912-2917	NN	denotes	liver
T1475	2918-2921	CC	denotes	and
T1476	2922-2927	NN	denotes	heart
T1477	2927-2929	,	denotes	,
T1478	2929-2932	CC	denotes	and
T1479	2933-2936	DT	denotes	the
T1481	2937-2944	JJS	denotes	highest
T1480	2945-2956	NNS	denotes	expressions
T1483	2957-2960	IN	denotes	for
T1484	2961-2966	NN	denotes	Acdp4
T1485	2967-2971	VBD	denotes	were
T1482	2972-2980	VBN	denotes	observed
T1486	2981-2983	IN	denotes	in
T1487	2984-2987	DT	denotes	the
T1488	2988-2994	NN	denotes	kidney
T1489	2994-2996	,	denotes	,
T1490	2996-3001	JJ	denotes	small
T1491	3002-3011	NN	denotes	intestine
T1492	3012-3015	CC	denotes	and
T1493	3016-3022	NN	denotes	testis
T1494	3022-3023	.	denotes	.
T1495	3023-3234	sentence	denotes	The expression levels for Acdp3 and 4 in skeleton muscle were barely detectable; however, β-actin showed normal expression suggesting that the results were not a consequence of bad RNA quality (data not shown).
T1496	3024-3027	DT	denotes	The
T1498	3028-3038	NN	denotes	expression
T1497	3039-3045	NNS	denotes	levels
T1500	3046-3049	IN	denotes	for
T1501	3050-3055	NN	denotes	Acdp3
T1502	3056-3059	CC	denotes	and
T1503	3060-3061	CD	denotes	4
T1504	3062-3064	IN	denotes	in
T1505	3065-3073	NN	denotes	skeleton
T1506	3074-3080	NN	denotes	muscle
T1499	3081-3085	VBD	denotes	were
T1508	3086-3092	RB	denotes	barely
T1509	3093-3103	JJ	denotes	detectable
T1510	3103-3104	:	denotes	;
T1511	3105-3112	RB	denotes	however
T1512	3112-3114	,	denotes	,
T1513	3114-3115	NN	denotes	β
T1515	3115-3116	HYPH	denotes	-
T1514	3116-3121	NN	denotes	actin
T1507	3122-3128	VBD	denotes	showed
T1516	3129-3135	JJ	denotes	normal
T1517	3136-3146	NN	denotes	expression
T1518	3147-3157	VBG	denotes	suggesting
T1519	3158-3162	IN	denotes	that
T1521	3163-3166	DT	denotes	the
T1522	3167-3174	NNS	denotes	results
T1520	3175-3179	VBD	denotes	were
T1523	3180-3183	RB	denotes	not
T1524	3184-3185	DT	denotes	a
T1525	3186-3197	NN	denotes	consequence
T1526	3198-3200	IN	denotes	of
T1527	3201-3204	JJ	denotes	bad
T1529	3205-3208	NN	denotes	RNA
T1528	3209-3216	NN	denotes	quality
T1530	3217-3218	-LRB-	denotes	(
T1532	3218-3222	NNS	denotes	data
T1533	3223-3226	RB	denotes	not
T1531	3227-3232	VBN	denotes	shown
T1534	3232-3233	-RRB-	denotes	)
T1535	3233-3234	.	denotes	.
T1536	3234-3463	sentence	denotes	The ubiquitous expression pattern may be taken as another indication of the functional importance of Acdp proteins in fundamental biological processes in addition to the sequence conservation in evolutionarily divergent species.
T1537	3235-3238	DT	denotes	The
T1539	3239-3249	JJ	denotes	ubiquitous
T1540	3250-3260	NN	denotes	expression
T1538	3261-3268	NN	denotes	pattern
T1542	3269-3272	MD	denotes	may
T1543	3273-3275	VB	denotes	be
T1541	3276-3281	VBN	denotes	taken
T1544	3282-3284	IN	denotes	as
T1545	3285-3292	DT	denotes	another
T1546	3293-3303	NN	denotes	indication
T1547	3304-3306	IN	denotes	of
T1548	3307-3310	DT	denotes	the
T1550	3311-3321	JJ	denotes	functional
T1549	3322-3332	NN	denotes	importance
T1551	3333-3335	IN	denotes	of
T1552	3336-3340	NN	denotes	Acdp
T1553	3341-3349	NN	denotes	proteins
T1554	3350-3352	IN	denotes	in
T1555	3353-3364	JJ	denotes	fundamental
T1557	3365-3375	JJ	denotes	biological
T1556	3376-3385	NNS	denotes	processes
T1558	3386-3388	IN	denotes	in
T1559	3389-3397	NN	denotes	addition
T1560	3398-3400	IN	denotes	to
T1561	3401-3404	DT	denotes	the
T1563	3405-3413	NN	denotes	sequence
T1562	3414-3426	NN	denotes	conservation
T1564	3427-3429	IN	denotes	in
T1565	3430-3444	RB	denotes	evolutionarily
T1566	3445-3454	JJ	denotes	divergent
T1567	3455-3462	NNS	denotes	species
T1568	3462-3463	.	denotes	.
T5731	3474-3482	NNP	denotes	Northern
T5732	3483-3487	NN	denotes	blot
T5733	3488-3496	NNS	denotes	analyses
T5734	3497-3499	IN	denotes	of
T5735	3500-3503	DT	denotes	the
T5737	3504-3508	NN	denotes	Acdp
T5738	3509-3513	NN	denotes	gene
T5736	3514-3520	NN	denotes	family
T5739	3520-3521	.	denotes	.
T5740	3521-3563	sentence	denotes	S. muscle represents skeletal muscle, Sm.
T5741	3522-3524	NN	denotes	S.
T5742	3525-3531	NN	denotes	muscle
T5743	3532-3542	VBZ	denotes	represents
T5744	3543-3551	JJ	denotes	skeletal
T5745	3552-3558	NN	denotes	muscle
T5746	3558-3560	,	denotes	,
T5747	3560-3562	NN	denotes	Sm
T5748	3562-3563	.	denotes	.
T5749	3563-3596	sentence	denotes	Int. represents small intestine.
T5750	3564-3568	NN	denotes	Int.
T5751	3569-3579	VBZ	denotes	represents
T5752	3580-3585	JJ	denotes	small
T5753	3586-3595	NN	denotes	intestine
T5754	3595-3596	.	denotes	.
T5755	3596-3663	sentence	denotes	Multiple Choice Northern Blot filters were purchased from Origene.
T5756	3597-3605	JJ	denotes	Multiple
T5757	3606-3612	NN	denotes	Choice
T5759	3613-3621	NNP	denotes	Northern
T5758	3622-3626	NN	denotes	Blot
T5760	3627-3634	NNS	denotes	filters
T5762	3635-3639	VBD	denotes	were
T5761	3640-3649	VBN	denotes	purchased
T5763	3650-3654	IN	denotes	from
T5764	3655-3662	NNP	denotes	Origene
T5765	3662-3663	.	denotes	.
T1609	3665-3676	JJ	denotes	Chromosomal
T1610	3677-3685	NN	denotes	location
T1611	3685-3838	sentence	denotes	Radiation hybrid mapping indicated that the Acdp1 gene maps to chromosome 19 between markers D19Mit119 (34.3 cR proximal)and D19Mit112 (13.6 cR distal).
T1612	3686-3695	NN	denotes	Radiation
T1614	3696-3702	NN	denotes	hybrid
T1613	3703-3710	NN	denotes	mapping
T1615	3711-3720	VBD	denotes	indicated
T1616	3721-3725	IN	denotes	that
T1618	3726-3729	DT	denotes	the
T1620	3730-3735	NN	denotes	Acdp1
T1619	3736-3740	NN	denotes	gene
T1617	3741-3745	VBZ	denotes	maps
T1621	3746-3748	IN	denotes	to
T1622	3749-3759	NN	denotes	chromosome
T1623	3760-3762	CD	denotes	19
T1624	3763-3770	IN	denotes	between
T1625	3771-3778	NNS	denotes	markers
T1626	3779-3788	NN	denotes	D19Mit119
T1627	3789-3790	-LRB-	denotes	(
T1629	3790-3794	CD	denotes	34.3
T1628	3795-3797	NN	denotes	cR
T1630	3798-3806	JJ	denotes	proximal
T1631	3806-3807	-RRB-	denotes	)
T1632	3807-3810	CC	denotes	and
T1633	3811-3820	NN	denotes	D19Mit112
T1634	3821-3822	-LRB-	denotes	(
T1636	3822-3826	CD	denotes	13.6
T1635	3827-3829	NN	denotes	cR
T1637	3830-3836	JJ	denotes	distal
T1638	3836-3837	-RRB-	denotes	)
T1639	3837-3838	.	denotes	.
T1640	3838-3974	sentence	denotes	The Acdp2 gene maps slightly more distal to the Acdp1 on chromosome 19 between D19Mit9 (2.4 cR proximal) and D19Mit38 (15.1 cR distal).
T1641	3839-3842	DT	denotes	The
T1643	3843-3848	NN	denotes	Acdp2
T1642	3849-3853	NN	denotes	gene
T1644	3854-3858	VBZ	denotes	maps
T1645	3859-3867	RB	denotes	slightly
T1646	3868-3872	RBR	denotes	more
T1647	3873-3879	JJ	denotes	distal
T1648	3880-3882	IN	denotes	to
T1649	3883-3886	DT	denotes	the
T1650	3887-3892	NN	denotes	Acdp1
T1651	3893-3895	IN	denotes	on
T1652	3896-3906	NN	denotes	chromosome
T1653	3907-3909	CD	denotes	19
T1654	3910-3917	IN	denotes	between
T1655	3918-3925	NN	denotes	D19Mit9
T1656	3926-3927	-LRB-	denotes	(
T1658	3927-3930	CD	denotes	2.4
T1657	3931-3933	NN	denotes	cR
T1659	3934-3942	JJ	denotes	proximal
T1660	3942-3943	-RRB-	denotes	)
T1661	3944-3947	CC	denotes	and
T1662	3948-3956	NN	denotes	D19Mit38
T1663	3957-3958	-LRB-	denotes	(
T1665	3958-3962	CD	denotes	15.1
T1664	3963-3965	NN	denotes	cR
T1666	3966-3972	JJ	denotes	distal
T1667	3972-3973	-RRB-	denotes	)
T1668	3973-3974	.	denotes	.
T1669	3974-4095	sentence	denotes	The Acdp3 and Acdp4 genes map to chromosome 1 within one BAC clone (RP23-294I17), proximal to marker D1Mit171 (17.4 cR).
T1670	3975-3978	DT	denotes	The
T1672	3979-3984	NN	denotes	Acdp3
T1673	3985-3988	CC	denotes	and
T1674	3989-3994	NN	denotes	Acdp4
T1671	3995-4000	NNS	denotes	genes
T1675	4001-4004	VBP	denotes	map
T1676	4005-4007	IN	denotes	to
T1677	4008-4018	NN	denotes	chromosome
T1678	4019-4020	CD	denotes	1
T1679	4021-4027	IN	denotes	within
T1680	4028-4031	CD	denotes	one
T1682	4032-4035	NN	denotes	BAC
T1681	4036-4041	NN	denotes	clone
T1683	4042-4043	-LRB-	denotes	(
T1685	4043-4047	NN	denotes	RP23
T1686	4047-4048	HYPH	denotes	-
T1684	4048-4054	NN	denotes	294I17
T1687	4054-4055	-RRB-	denotes	)
T1688	4055-4057	,	denotes	,
T1689	4057-4065	JJ	denotes	proximal
T1690	4066-4068	IN	denotes	to
T1691	4069-4075	NN	denotes	marker
T1692	4076-4084	NN	denotes	D1Mit171
T1693	4085-4086	-LRB-	denotes	(
T1695	4086-4090	CD	denotes	17.4
T1694	4091-4093	NN	denotes	cR
T1696	4093-4094	-RRB-	denotes	)
T1697	4094-4095	.	denotes	.
T1698	4095-4149	sentence	denotes	These regions are syntenic to the human counterparts.
T1699	4096-4101	DT	denotes	These
T1700	4102-4109	NNS	denotes	regions
T1701	4110-4113	VBP	denotes	are
T1702	4114-4122	JJ	denotes	syntenic
T1703	4123-4125	IN	denotes	to
T1704	4126-4129	DT	denotes	the
T1706	4130-4135	JJ	denotes	human
T1705	4136-4148	NNS	denotes	counterparts
T1707	4148-4149	.	denotes	.
T2019	4151-4159	NN	denotes	Sequence
T2020	4160-4168	NN	denotes	homology
T2021	4169-4172	CC	denotes	and
T2022	4173-4182	JJ	denotes	molecular
T2023	4183-4198	NNS	denotes	characteristics
T2024	4198-4320	sentence	denotes	The mouse Acdp genes showed very strong homologies of both nucleotide and AA sequences to the human ACDP genes (Table 1).
T2025	4199-4202	DT	denotes	The
T2027	4203-4208	NN	denotes	mouse
T2028	4209-4213	NN	denotes	Acdp
T2026	4214-4219	NNS	denotes	genes
T2029	4220-4226	VBD	denotes	showed
T2030	4227-4231	RB	denotes	very
T2031	4232-4238	JJ	denotes	strong
T2032	4239-4249	NNS	denotes	homologies
T2033	4250-4252	IN	denotes	of
T2034	4253-4257	CC	denotes	both
T2035	4258-4268	NN	denotes	nucleotide
T2037	4269-4272	CC	denotes	and
T2038	4273-4275	NN	denotes	AA
T2036	4276-4285	NNS	denotes	sequences
T2039	4286-4288	IN	denotes	to
T2040	4289-4292	DT	denotes	the
T2042	4293-4298	JJ	denotes	human
T2043	4299-4303	NN	denotes	ACDP
T2041	4304-4309	NNS	denotes	genes
T2044	4310-4311	-LRB-	denotes	(
T2045	4311-4316	NN	denotes	Table
T2046	4317-4318	CD	denotes	1
T2047	4318-4319	-RRB-	denotes	)
T2048	4319-4320	.	denotes	.
T2049	4320-4481	sentence	denotes	The highest homologies were observed between the human ACDP2 and the mouse Acdp2 gene (91% of nucleotide identity, 97% of AA identity and 99.4% of AA homology).
T2050	4321-4324	DT	denotes	The
T2052	4325-4332	JJS	denotes	highest
T2051	4333-4343	NNS	denotes	homologies
T2054	4344-4348	VBD	denotes	were
T2053	4349-4357	VBN	denotes	observed
T2055	4358-4365	IN	denotes	between
T2056	4366-4369	DT	denotes	the
T2058	4370-4375	JJ	denotes	human
T2057	4376-4381	NN	denotes	ACDP2
T2059	4382-4385	CC	denotes	and
T2060	4386-4389	DT	denotes	the
T2062	4390-4395	NN	denotes	mouse
T2061	4396-4401	NN	denotes	Acdp2
T2063	4402-4406	NN	denotes	gene
T2064	4407-4408	-LRB-	denotes	(
T2066	4408-4410	CD	denotes	91
T2065	4410-4411	NN	denotes	%
T2067	4412-4414	IN	denotes	of
T2068	4415-4425	NN	denotes	nucleotide
T2069	4426-4434	NN	denotes	identity
T2070	4434-4436	,	denotes	,
T2071	4436-4438	CD	denotes	97
T2072	4438-4439	NN	denotes	%
T2073	4440-4442	IN	denotes	of
T2074	4443-4445	NN	denotes	AA
T2075	4446-4454	NN	denotes	identity
T2076	4455-4458	CC	denotes	and
T2077	4459-4463	CD	denotes	99.4
T2078	4463-4464	NN	denotes	%
T2079	4465-4467	IN	denotes	of
T2080	4468-4470	NN	denotes	AA
T2081	4471-4479	NN	denotes	homology
T2082	4479-4480	-RRB-	denotes	)
T2083	4480-4481	.	denotes	.
T2084	4481-4702	sentence	denotes	In addition, the 5' UTR nucleotide sequences (20 bp of nucleotides before start codon) also showed high homologies to the human homologs, for example, the Acdp2 5' UTR sequence showed 95% identities to its human homolog.
T2085	4482-4484	IN	denotes	In
T2087	4485-4493	NN	denotes	addition
T2088	4493-4495	,	denotes	,
T2089	4495-4498	DT	denotes	the
T2091	4499-4500	CD	denotes	5
T2093	4500-4501	SYM	denotes	'
T2092	4502-4505	NN	denotes	UTR
T2094	4506-4516	NN	denotes	nucleotide
T2090	4517-4526	NNS	denotes	sequences
T2095	4527-4528	-LRB-	denotes	(
T2097	4528-4530	CD	denotes	20
T2096	4531-4533	NNS	denotes	bp
T2098	4534-4536	IN	denotes	of
T2099	4537-4548	NNS	denotes	nucleotides
T2100	4549-4555	IN	denotes	before
T2101	4556-4561	NN	denotes	start
T2102	4562-4567	NN	denotes	codon
T2103	4567-4568	-RRB-	denotes	)
T2104	4569-4573	RB	denotes	also
T2086	4574-4580	VBD	denotes	showed
T2106	4581-4585	JJ	denotes	high
T2107	4586-4596	NNS	denotes	homologies
T2108	4597-4599	IN	denotes	to
T2109	4600-4603	DT	denotes	the
T2111	4604-4609	JJ	denotes	human
T2110	4610-4618	NNS	denotes	homologs
T2112	4618-4620	,	denotes	,
T2113	4620-4623	IN	denotes	for
T2114	4624-4631	NN	denotes	example
T2115	4631-4633	,	denotes	,
T2116	4633-4636	DT	denotes	the
T2118	4637-4642	NN	denotes	Acdp2
T2119	4643-4644	CD	denotes	5
T2121	4644-4645	SYM	denotes	'
T2120	4646-4649	NN	denotes	UTR
T2117	4650-4658	NN	denotes	sequence
T2105	4659-4665	VBD	denotes	showed
T2122	4666-4668	CD	denotes	95
T2123	4668-4669	NN	denotes	%
T2124	4670-4680	NNS	denotes	identities
T2125	4681-4683	IN	denotes	to
T2126	4684-4687	PRP$	denotes	its
T2128	4688-4693	JJ	denotes	human
T2127	4694-4701	NN	denotes	homolog
T2129	4701-4702	.	denotes	.
T2130	4702-4884	sentence	denotes	However, the homologies in the 3' UTR sequences (20 bp of nucleotides after stop codon) were much lower (40–55%) for all Acdp genes except Acdp4 (90% identity to its human homolog).
T2131	4703-4710	RB	denotes	However
T2133	4710-4712	,	denotes	,
T2134	4712-4715	DT	denotes	the
T2135	4716-4726	NNS	denotes	homologies
T2136	4727-4729	IN	denotes	in
T2137	4730-4733	DT	denotes	the
T2139	4734-4735	CD	denotes	3
T2141	4735-4736	SYM	denotes	'
T2140	4737-4740	NN	denotes	UTR
T2138	4741-4750	NNS	denotes	sequences
T2142	4751-4752	-LRB-	denotes	(
T2144	4752-4754	CD	denotes	20
T2143	4755-4757	NNS	denotes	bp
T2145	4758-4760	IN	denotes	of
T2146	4761-4772	NNS	denotes	nucleotides
T2147	4773-4778	IN	denotes	after
T2148	4779-4783	NN	denotes	stop
T2149	4784-4789	NN	denotes	codon
T2150	4789-4790	-RRB-	denotes	)
T2132	4791-4795	VBD	denotes	were
T2151	4796-4800	RB	denotes	much
T2152	4801-4806	JJR	denotes	lower
T2153	4807-4808	-LRB-	denotes	(
T2155	4808-4810	CD	denotes	40
T2157	4810-4811	SYM	denotes	–
T2156	4811-4813	CD	denotes	55
T2154	4813-4814	NN	denotes	%
T2158	4814-4815	-RRB-	denotes	)
T2159	4816-4819	IN	denotes	for
T2160	4820-4823	DT	denotes	all
T2162	4824-4828	NN	denotes	Acdp
T2161	4829-4834	NNS	denotes	genes
T2163	4835-4841	IN	denotes	except
T2164	4842-4847	NN	denotes	Acdp4
T2165	4848-4849	-LRB-	denotes	(
T2167	4849-4851	CD	denotes	90
T2168	4851-4852	NN	denotes	%
T2166	4853-4861	NN	denotes	identity
T2169	4862-4864	IN	denotes	to
T2170	4865-4868	PRP$	denotes	its
T2172	4869-4874	JJ	denotes	human
T2171	4875-4882	NN	denotes	homolog
T2173	4882-4883	-RRB-	denotes	)
T2174	4883-4884	.	denotes	.
T2175	4884-5018	sentence	denotes	The ancient conserved domain (ACD) has 55.3% of AA identity and 83.3% of homology between all mouse and human ACDP proteins (Fig. 2).
T2176	4885-4888	DT	denotes	The
T2178	4889-4896	JJ	denotes	ancient
T2179	4897-4906	VBN	denotes	conserved
T2177	4907-4913	NN	denotes	domain
T2181	4914-4915	-LRB-	denotes	(
T2182	4915-4918	NN	denotes	ACD
T2183	4918-4919	-RRB-	denotes	)
T2180	4920-4923	VBZ	denotes	has
T2184	4924-4928	CD	denotes	55.3
T2185	4928-4929	NN	denotes	%
T2186	4930-4932	IN	denotes	of
T2187	4933-4935	NN	denotes	AA
T2188	4936-4944	NN	denotes	identity
T2189	4945-4948	CC	denotes	and
T2190	4949-4953	CD	denotes	83.3
T2191	4953-4954	NN	denotes	%
T2192	4955-4957	IN	denotes	of
T2193	4958-4966	NN	denotes	homology
T2194	4967-4974	IN	denotes	between
T2195	4975-4978	DT	denotes	all
T2197	4979-4984	NN	denotes	mouse
T2198	4985-4988	CC	denotes	and
T2199	4989-4994	JJ	denotes	human
T2200	4995-4999	NN	denotes	ACDP
T2196	5000-5008	NN	denotes	proteins
T2201	5009-5010	-LRB-	denotes	(
T2202	5010-5014	NN	denotes	Fig.
T2203	5015-5016	CD	denotes	2
T2204	5016-5017	-RRB-	denotes	)
T2205	5017-5018	.	denotes	.
T2206	5018-5166	sentence	denotes	The ACD domain is evolutionarily conserved in divergent species ranging from bacteria, yeast, C. elegans, D. melanogaster, mouse to human (Fig. 3).
T2207	5019-5022	DT	denotes	The
T2209	5023-5026	NN	denotes	ACD
T2208	5027-5033	NN	denotes	domain
T2211	5034-5036	VBZ	denotes	is
T2212	5037-5051	RB	denotes	evolutionarily
T2210	5052-5061	VBN	denotes	conserved
T2213	5062-5064	IN	denotes	in
T2214	5065-5074	JJ	denotes	divergent
T2215	5075-5082	NNS	denotes	species
T2216	5083-5090	VBG	denotes	ranging
T2217	5091-5095	IN	denotes	from
T2218	5096-5104	NNS	denotes	bacteria
T2219	5104-5106	,	denotes	,
T2220	5106-5111	NN	denotes	yeast
T2221	5111-5113	,	denotes	,
T2222	5113-5115	NNP	denotes	C.
T2223	5116-5123	NNP	denotes	elegans
T2224	5123-5125	,	denotes	,
T2225	5125-5127	NNP	denotes	D.
T2226	5128-5140	NNP	denotes	melanogaster
T2227	5140-5142	,	denotes	,
T2228	5142-5147	NN	denotes	mouse
T2229	5148-5150	IN	denotes	to
T2230	5151-5156	JJ	denotes	human
T2231	5157-5158	-LRB-	denotes	(
T2232	5158-5162	NN	denotes	Fig.
T2233	5163-5164	CD	denotes	3
T2234	5164-5165	-RRB-	denotes	)
T2235	5165-5166	.	denotes	.
T2236	5166-5361	sentence	denotes	Particularly, as shown in Fig. 3, Acdp proteins showed very strong AA homology to bacteria CorC protein (35% AA identity with 55% homology), which is involved in magnesium and cobalt efflux [7].
T2237	5167-5179	RB	denotes	Particularly
T2239	5179-5181	,	denotes	,
T2240	5181-5183	IN	denotes	as
T2241	5184-5189	VBN	denotes	shown
T2242	5190-5192	IN	denotes	in
T2243	5193-5197	NN	denotes	Fig.
T2244	5198-5199	CD	denotes	3
T2245	5199-5201	,	denotes	,
T2246	5201-5205	NN	denotes	Acdp
T2247	5206-5214	NN	denotes	proteins
T2238	5215-5221	VBD	denotes	showed
T2248	5222-5226	RB	denotes	very
T2249	5227-5233	JJ	denotes	strong
T2251	5234-5236	NN	denotes	AA
T2250	5237-5245	NN	denotes	homology
T2252	5246-5248	IN	denotes	to
T2253	5249-5257	NNS	denotes	bacteria
T2255	5258-5262	NN	denotes	CorC
T2254	5263-5270	NN	denotes	protein
T2256	5271-5272	-LRB-	denotes	(
T2258	5272-5274	CD	denotes	35
T2259	5274-5275	NN	denotes	%
T2260	5276-5278	NN	denotes	AA
T2257	5279-5287	NN	denotes	identity
T2261	5288-5292	IN	denotes	with
T2262	5293-5295	CD	denotes	55
T2263	5295-5296	NN	denotes	%
T2264	5297-5305	NN	denotes	homology
T2265	5305-5306	-RRB-	denotes	)
T2266	5306-5308	,	denotes	,
T2267	5308-5313	WDT	denotes	which
T2269	5314-5316	VBZ	denotes	is
T2268	5317-5325	VBN	denotes	involved
T2270	5326-5328	IN	denotes	in
T2271	5329-5338	NN	denotes	magnesium
T2273	5339-5342	CC	denotes	and
T2274	5343-5349	NN	denotes	cobalt
T2272	5350-5356	NN	denotes	efflux
T2275	5357-5358	-LRB-	denotes	[
T2276	5358-5359	CD	denotes	7
T2277	5359-5360	-RRB-	denotes	]
T2278	5360-5361	.	denotes	.
T2279	5361-5487	sentence	denotes	High AA homology was also observed between the Acdp proteins and the yeast Amip3 protein (35% AA identity with 56% homology).
T2280	5362-5366	JJ	denotes	High
T2282	5367-5369	NN	denotes	AA
T2281	5370-5378	NN	denotes	homology
T2284	5379-5382	VBD	denotes	was
T2285	5383-5387	RB	denotes	also
T2283	5388-5396	VBN	denotes	observed
T2286	5397-5404	IN	denotes	between
T2287	5405-5408	DT	denotes	the
T2289	5409-5413	NN	denotes	Acdp
T2288	5414-5422	NN	denotes	proteins
T2290	5423-5426	CC	denotes	and
T2291	5427-5430	DT	denotes	the
T2293	5431-5436	NN	denotes	yeast
T2294	5437-5442	NN	denotes	Amip3
T2292	5443-5450	NN	denotes	protein
T2295	5451-5452	-LRB-	denotes	(
T2297	5452-5454	CD	denotes	35
T2298	5454-5455	NN	denotes	%
T2299	5456-5458	NN	denotes	AA
T2296	5459-5467	NN	denotes	identity
T2300	5468-5472	IN	denotes	with
T2301	5473-5475	CD	denotes	56
T2303	5475-5476	NN	denotes	%
T2302	5477-5485	NN	denotes	homology
T2304	5485-5486	-RRB-	denotes	)
T2305	5486-5487	.	denotes	.
T2306	5487-5556	sentence	denotes	The Amip3 is likely to be a homologous to the bacteria CorC protein.
T2307	5488-5491	DT	denotes	The
T2308	5492-5497	NN	denotes	Amip3
T2309	5498-5500	VBZ	denotes	is
T2310	5501-5507	JJ	denotes	likely
T2311	5508-5510	TO	denotes	to
T2312	5511-5513	VB	denotes	be
T2313	5514-5515	DT	denotes	a
T2314	5516-5526	JJ	denotes	homologous
T2315	5527-5529	IN	denotes	to
T2316	5530-5533	DT	denotes	the
T2318	5534-5542	NNS	denotes	bacteria
T2319	5543-5547	NN	denotes	CorC
T2317	5548-5555	NN	denotes	protein
T2320	5555-5556	.	denotes	.
T2321	5556-5708	sentence	denotes	The Amip3 mutants confer resistance to copper toxicity (Personal communication with Dr. V.C. Culotte, John Hopkins Bloomberg, School of Public Health).
T2322	5557-5560	DT	denotes	The
T2324	5561-5566	NN	denotes	Amip3
T2323	5567-5574	NNS	denotes	mutants
T2325	5575-5581	VBP	denotes	confer
T2326	5582-5592	NN	denotes	resistance
T2327	5593-5595	IN	denotes	to
T2328	5596-5602	NN	denotes	copper
T2329	5603-5611	NN	denotes	toxicity
T2330	5612-5613	-LRB-	denotes	(
T2332	5613-5621	JJ	denotes	Personal
T2333	5622-5635	NN	denotes	communication
T2334	5636-5640	IN	denotes	with
T2331	5641-5644	NNP	denotes	Dr.
T2335	5645-5649	NNP	denotes	V.C.
T2336	5650-5657	NNP	denotes	Culotte
T2337	5657-5659	,	denotes	,
T2338	5659-5663	NNP	denotes	John
T2339	5664-5671	NNP	denotes	Hopkins
T2340	5672-5681	NNP	denotes	Bloomberg
T2341	5681-5683	,	denotes	,
T2342	5683-5689	NNP	denotes	School
T2343	5690-5692	IN	denotes	of
T2344	5693-5699	NNP	denotes	Public
T2345	5700-5706	NNP	denotes	Health
T2346	5706-5707	-RRB-	denotes	)
T2347	5707-5708	.	denotes	.
T2348	5708-5858	sentence	denotes	The evolutionary relationships among those proteins are illustrated by a phylogenetic tree constructed based on the AA homology of proteins (Fig. 4).
T2349	5709-5712	DT	denotes	The
T2351	5713-5725	JJ	denotes	evolutionary
T2350	5726-5739	NNS	denotes	relationships
T2353	5740-5745	IN	denotes	among
T2354	5746-5751	DT	denotes	those
T2355	5752-5760	NN	denotes	proteins
T2356	5761-5764	VBP	denotes	are
T2352	5765-5776	VBN	denotes	illustrated
T2357	5777-5779	IN	denotes	by
T2358	5780-5781	DT	denotes	a
T2360	5782-5794	JJ	denotes	phylogenetic
T2359	5795-5799	NN	denotes	tree
T2361	5800-5811	VBN	denotes	constructed
T2362	5812-5817	VBN	denotes	based
T2363	5818-5820	IN	denotes	on
T2364	5821-5824	DT	denotes	the
T2366	5825-5827	NN	denotes	AA
T2365	5828-5836	NN	denotes	homology
T2367	5837-5839	IN	denotes	of
T2368	5840-5848	NN	denotes	proteins
T2369	5849-5850	-LRB-	denotes	(
T2370	5850-5854	NN	denotes	Fig.
T2371	5855-5856	CD	denotes	4
T2372	5856-5857	-RRB-	denotes	)
T2373	5857-5858	.	denotes	.
T2374	5858-5859	sentence	denotes
T6623	5868-5878	NN	denotes	Nucleotide
T6625	5879-5882	CC	denotes	and
T6626	5883-5888	NN	denotes	amino
T6627	5889-5893	NN	denotes	acid
T6624	5894-5904	NNS	denotes	homologies
T6628	5905-5906	-LRB-	denotes	(
T6629	5906-5907	NN	denotes	%
T6630	5907-5908	-RRB-	denotes	)
T6631	5909-5916	IN	denotes	between
T6632	5917-5922	JJ	denotes	human
T6633	5923-5927	NN	denotes	ACDP
T6635	5928-5931	CC	denotes	and
T6636	5932-5937	NN	denotes	mouse
T6637	5938-5942	NN	denotes	Acdp
T6634	5943-5950	NNS	denotes	members
T6638	5950-5951	.	denotes	.
T5826	6138-6143	NN	denotes	Amino
T5827	6144-6148	NN	denotes	acid
T5828	6149-6157	NN	denotes	sequence
T5830	6158-6166	NN	denotes	homology
T5829	6167-6176	NN	denotes	alignment
T5831	6177-6180	IN	denotes	for
T5832	6181-6184	DT	denotes	all
T5833	6185-6187	IN	denotes	of
T5834	6188-6191	DT	denotes	the
T5836	6192-6196	NN	denotes	ACDP
T5837	6197-6200	CC	denotes	and
T5838	6201-6205	NN	denotes	Acdp
T5835	6206-6211	NNS	denotes	genes
T5839	6212-6218	IN	denotes	within
T5840	6219-6222	DT	denotes	the
T5842	6223-6226	NN	denotes	ACD
T5841	6227-6233	NN	denotes	domain
T5843	6233-6234	.	denotes	.
T5844	6234-6400	sentence	denotes	The sequence data for the Acdp genes have been deposited in GenBank under accession number AF202994 (Acdp1), AF216961 (Acdp2), AF216964 (Acdp3) and AF216963 (Acdp4).
T5845	6235-6238	DT	denotes	The
T5847	6239-6247	NN	denotes	sequence
T5846	6248-6252	NNS	denotes	data
T5849	6253-6256	IN	denotes	for
T5850	6257-6260	DT	denotes	the
T5852	6261-6265	NN	denotes	Acdp
T5851	6266-6271	NNS	denotes	genes
T5853	6272-6276	VBP	denotes	have
T5854	6277-6281	VBN	denotes	been
T5848	6282-6291	VBN	denotes	deposited
T5855	6292-6294	IN	denotes	in
T5856	6295-6302	NNP	denotes	GenBank
T5857	6303-6308	IN	denotes	under
T5858	6309-6318	NN	denotes	accession
T5860	6319-6325	NN	denotes	number
T5859	6326-6334	NN	denotes	AF202994
T5861	6335-6336	-LRB-	denotes	(
T5862	6336-6341	NN	denotes	Acdp1
T5863	6341-6342	-RRB-	denotes	)
T5864	6342-6344	,	denotes	,
T5865	6344-6352	NN	denotes	AF216961
T5866	6353-6354	-LRB-	denotes	(
T5867	6354-6359	NN	denotes	Acdp2
T5868	6359-6360	-RRB-	denotes	)
T5869	6360-6362	,	denotes	,
T5870	6362-6370	NN	denotes	AF216964
T5871	6371-6372	-LRB-	denotes	(
T5872	6372-6377	NN	denotes	Acdp3
T5873	6377-6378	-RRB-	denotes	)
T5874	6379-6382	CC	denotes	and
T5875	6383-6391	NN	denotes	AF216963
T5876	6392-6393	-LRB-	denotes	(
T5877	6393-6398	NN	denotes	Acdp4
T5878	6398-6399	-RRB-	denotes	)
T5879	6399-6400	.	denotes	.
T5880	6400-6503	sentence	denotes	Identical amino acids or amino acids with very strong homologies among all proteins were shaded black.
T5881	6401-6410	JJ	denotes	Identical
T5883	6411-6416	NN	denotes	amino
T5882	6417-6422	NNS	denotes	acids
T5885	6423-6425	CC	denotes	or
T5886	6426-6431	NN	denotes	amino
T5887	6432-6437	NNS	denotes	acids
T5888	6438-6442	IN	denotes	with
T5889	6443-6447	RB	denotes	very
T5890	6448-6454	JJ	denotes	strong
T5891	6455-6465	NNS	denotes	homologies
T5892	6466-6471	IN	denotes	among
T5893	6472-6475	DT	denotes	all
T5894	6476-6484	NN	denotes	proteins
T5895	6485-6489	VBD	denotes	were
T5884	6490-6496	VBN	denotes	shaded
T5896	6497-6502	JJ	denotes	black
T5897	6502-6503	.	denotes	.
T5898	6503-6610	sentence	denotes	Identical amino acids or amino acids with very strong homologies in most of the proteins were shaded grey.
T5899	6504-6513	JJ	denotes	Identical
T5901	6514-6519	NN	denotes	amino
T5900	6520-6525	NNS	denotes	acids
T5903	6526-6528	CC	denotes	or
T5904	6529-6534	NN	denotes	amino
T5905	6535-6540	NNS	denotes	acids
T5906	6541-6545	IN	denotes	with
T5907	6546-6550	RB	denotes	very
T5908	6551-6557	JJ	denotes	strong
T5909	6558-6568	NNS	denotes	homologies
T5910	6569-6571	IN	denotes	in
T5911	6572-6576	JJS	denotes	most
T5912	6577-6579	IN	denotes	of
T5913	6580-6583	DT	denotes	the
T5914	6584-6592	NN	denotes	proteins
T5915	6593-6597	VBD	denotes	were
T5902	6598-6604	VBN	denotes	shaded
T5916	6605-6609	JJ	denotes	grey
T5917	6609-6610	.	denotes	.
T5918	6610-6654	sentence	denotes	Dot lines represent gaps for the alignment.
T5919	6611-6614	NN	denotes	Dot
T5920	6615-6620	NNS	denotes	lines
T5921	6621-6630	VBP	denotes	represent
T5922	6631-6635	NNS	denotes	gaps
T5923	6636-6639	IN	denotes	for
T5924	6640-6643	DT	denotes	the
T5925	6644-6653	NN	denotes	alignment
T5926	6653-6654	.	denotes	.
T6010	6665-6670	NN	denotes	Amino
T6011	6671-6675	NN	denotes	acid
T6013	6676-6684	NN	denotes	sequence
T6012	6685-6694	NN	denotes	alignment
T6014	6695-6702	VBG	denotes	showing
T6015	6703-6706	DT	denotes	the
T6016	6707-6719	NN	denotes	conservation
T6017	6720-6722	IN	denotes	of
T6018	6723-6726	NN	denotes	ACD
T6019	6727-6733	NN	denotes	domain
T6020	6734-6736	IN	denotes	in
T6021	6737-6744	JJ	denotes	various
T6022	6745-6752	NNS	denotes	species
T6023	6752-6753	.	denotes	.
T6024	6753-6815	sentence	denotes	Amip3 is a protein from Saccharomyces cerevisiae (NP_014581).
T6025	6754-6759	NN	denotes	Amip3
T6026	6760-6762	VBZ	denotes	is
T6027	6763-6764	DT	denotes	a
T6028	6765-6772	NN	denotes	protein
T6029	6773-6777	IN	denotes	from
T6030	6778-6791	NNP	denotes	Saccharomyces
T6031	6792-6802	NNP	denotes	cerevisiae
T6032	6803-6804	-LRB-	denotes	(
T6033	6804-6813	NN	denotes	NP_014581
T6034	6813-6814	-RRB-	denotes	)
T6035	6814-6815	.	denotes	.
T6036	6815-6867	sentence	denotes	CanG is a protein from Candida glabrata (AAF33142).
T6037	6816-6820	NN	denotes	CanG
T6038	6821-6823	VBZ	denotes	is
T6039	6824-6825	DT	denotes	a
T6040	6826-6833	NN	denotes	protein
T6041	6834-6838	IN	denotes	from
T6042	6839-6846	NNP	denotes	Candida
T6043	6847-6855	NNP	denotes	glabrata
T6044	6856-6857	-LRB-	denotes	(
T6045	6857-6865	NN	denotes	AAF33142
T6046	6865-6866	-RRB-	denotes	)
T6047	6866-6867	.	denotes	.
T6048	6867-6933	sentence	denotes	NeuC (EAA31204) is a hypothetical protein from Neurospora crassa.
T6049	6868-6872	NN	denotes	NeuC
T6051	6873-6874	-LRB-	denotes	(
T6052	6874-6882	NN	denotes	EAA31204
T6053	6882-6883	-RRB-	denotes	)
T6050	6884-6886	VBZ	denotes	is
T6054	6887-6888	DT	denotes	a
T6056	6889-6901	JJ	denotes	hypothetical
T6055	6902-6909	NN	denotes	protein
T6057	6910-6914	IN	denotes	from
T6058	6915-6925	NNP	denotes	Neurospora
T6059	6926-6932	NNP	denotes	crassa
T6060	6932-6933	.	denotes	.
T6061	6933-6978	sentence	denotes	DroM is a gene product from D. melanogaster.
T6062	6934-6938	NN	denotes	DroM
T6063	6939-6941	VBZ	denotes	is
T6064	6942-6943	DT	denotes	a
T6066	6944-6948	NN	denotes	gene
T6065	6949-6956	NN	denotes	product
T6067	6957-6961	IN	denotes	from
T6068	6962-6964	NNP	denotes	D.
T6069	6965-6977	NNP	denotes	melanogaster
T6070	6977-6978	.	denotes	.
T6071	6978-7070	sentence	denotes	The accession number for this gene is CG40084 in BDGP (Berkeley Drosophila Genome Project).
T6072	6979-6982	DT	denotes	The
T6074	6983-6992	NN	denotes	accession
T6073	6993-6999	NN	denotes	number
T6076	7000-7003	IN	denotes	for
T6077	7004-7008	DT	denotes	this
T6078	7009-7013	NN	denotes	gene
T6075	7014-7016	VBZ	denotes	is
T6079	7017-7024	NN	denotes	CG40084
T6080	7025-7027	IN	denotes	in
T6081	7028-7032	NN	denotes	BDGP
T6082	7033-7034	-LRB-	denotes	(
T6083	7034-7042	NNP	denotes	Berkeley
T6085	7043-7053	NNP	denotes	Drosophila
T6086	7054-7060	NNP	denotes	Genome
T6084	7061-7068	NNP	denotes	Project
T6087	7068-7069	-RRB-	denotes	)
T6088	7069-7070	.	denotes	.
T6089	7070-7140	sentence	denotes	AnoG represents a protein from the anopheles gambiae str. (EAA01004).
T6090	7071-7075	NN	denotes	AnoG
T6091	7076-7086	VBZ	denotes	represents
T6092	7087-7088	DT	denotes	a
T6093	7089-7096	NN	denotes	protein
T6094	7097-7101	IN	denotes	from
T6095	7102-7105	DT	denotes	the
T6097	7106-7115	NNP	denotes	anopheles
T6098	7116-7123	NNP	denotes	gambiae
T6096	7124-7128	NN	denotes	str.
T6099	7129-7130	-LRB-	denotes	(
T6100	7130-7138	NN	denotes	EAA01004
T6101	7138-7139	-RRB-	denotes	)
T6102	7139-7140	.	denotes	.
T6103	7140-7214	sentence	denotes	CaeE (AAK77203) is a hypothetical protein from the Caenohabditis elegans.
T6104	7141-7145	NN	denotes	CaeE
T6106	7146-7147	-LRB-	denotes	(
T6107	7147-7155	NN	denotes	AAK77203
T6108	7155-7156	-RRB-	denotes	)
T6105	7157-7159	VBZ	denotes	is
T6109	7160-7161	DT	denotes	a
T6111	7162-7174	JJ	denotes	hypothetical
T6110	7175-7182	NN	denotes	protein
T6112	7183-7187	IN	denotes	from
T6113	7188-7191	DT	denotes	the
T6115	7192-7205	NNP	denotes	Caenohabditis
T6114	7206-7213	NNP	denotes	elegans
T6116	7213-7214	.	denotes	.
T6117	7214-7307	sentence	denotes	CorC represents bacteria magnesium and cobalt efflux protein from the Shewanella oneidensis.
T6118	7215-7219	NN	denotes	CorC
T6119	7220-7230	VBZ	denotes	represents
T6120	7231-7239	NNS	denotes	bacteria
T6121	7240-7249	NN	denotes	magnesium
T6122	7250-7253	CC	denotes	and
T6123	7254-7260	NN	denotes	cobalt
T6125	7261-7267	NN	denotes	efflux
T6124	7268-7275	NN	denotes	protein
T6126	7276-7280	IN	denotes	from
T6127	7281-7284	DT	denotes	the
T6129	7285-7295	NNP	denotes	Shewanella
T6128	7296-7306	NNP	denotes	oneidensis
T6130	7306-7307	.	denotes	.
T6131	7307-7387	sentence	denotes	XyFD is a hypothetical protein from the Xylella fastidiosa Dixon (ZP_00038107).
T6132	7308-7312	NN	denotes	XyFD
T6133	7313-7315	VBZ	denotes	is
T6134	7316-7317	DT	denotes	a
T6136	7318-7330	JJ	denotes	hypothetical
T6135	7331-7338	NN	denotes	protein
T6137	7339-7343	IN	denotes	from
T6138	7344-7347	DT	denotes	the
T6140	7348-7355	NNP	denotes	Xylella
T6141	7356-7366	NNP	denotes	fastidiosa
T6139	7367-7372	NNP	denotes	Dixon
T6142	7373-7374	-LRB-	denotes	(
T6143	7374-7385	NN	denotes	ZP_00038107
T6144	7385-7386	-RRB-	denotes	)
T6145	7386-7387	.	denotes	.
T6153	7398-7410	JJ	denotes	Phylogenetic
T6154	7411-7415	NN	denotes	tree
T6155	7416-7423	VBG	denotes	showing
T6156	7424-7437	NNS	denotes	relationships
T6157	7438-7443	IN	denotes	among
T6158	7444-7452	NN	denotes	proteins
T6159	7453-7463	VBG	denotes	containing
T6160	7464-7467	DT	denotes	the
T6162	7468-7471	NN	denotes	ACD
T6161	7472-7478	NN	denotes	domain
T6163	7479-7483	IN	denotes	from
T6164	7484-7490	NN	denotes	figure
T6165	7491-7492	CD	denotes	2
T6166	7493-7496	CC	denotes	and
T6167	7497-7498	CD	denotes	3
T6168	7498-7499	.	denotes	.
T6169	7499-7612	sentence	denotes	The phylogenetic tree was constructed according to the calculation of the best match for the selected sequences.
T6170	7500-7503	DT	denotes	The
T6172	7504-7516	JJ	denotes	phylogenetic
T6171	7517-7521	NN	denotes	tree
T6174	7522-7525	VBD	denotes	was
T6173	7526-7537	VBN	denotes	constructed
T6175	7538-7547	VBG	denotes	according
T6176	7548-7550	IN	denotes	to
T6177	7551-7554	DT	denotes	the
T6178	7555-7566	NN	denotes	calculation
T6179	7567-7569	IN	denotes	of
T6180	7570-7573	DT	denotes	the
T6182	7574-7578	JJS	denotes	best
T6181	7579-7584	NN	denotes	match
T6183	7585-7588	IN	denotes	for
T6184	7589-7592	DT	denotes	the
T6186	7593-7601	JJ	denotes	selected
T6185	7602-7611	NNS	denotes	sequences
T6187	7611-7612	.	denotes	.
T6188	7612-7682	sentence	denotes	Abbreviations for each protein are the same as presented in figure 3.
T6189	7613-7626	NNS	denotes	Abbreviations
T6191	7627-7630	IN	denotes	for
T6192	7631-7635	DT	denotes	each
T6193	7636-7643	NN	denotes	protein
T6190	7644-7647	VBP	denotes	are
T6194	7648-7651	DT	denotes	the
T6195	7652-7656	JJ	denotes	same
T6196	7657-7659	IN	denotes	as
T6197	7660-7669	VBN	denotes	presented
T6198	7670-7672	IN	denotes	in
T6199	7673-7679	NN	denotes	figure
T6200	7680-7681	CD	denotes	3
T6201	7681-7682	.	denotes	.
T2376	7683-7685	PRP	denotes	We
T2375	7683-7866	sentence	denotes	We found that all mouse Acdp members contain four distinct transmembrane domains (Fig. 5), two CBS domains and a DUF21 domain that are found in bacteria CorC and yeast Amip3 proteins.
T2377	7686-7691	VBD	denotes	found
T2378	7692-7696	IN	denotes	that
T2380	7697-7700	DT	denotes	all
T2382	7701-7706	NN	denotes	mouse
T2383	7707-7711	NN	denotes	Acdp
T2381	7712-7719	NNS	denotes	members
T2379	7720-7727	VBP	denotes	contain
T2384	7728-7732	CD	denotes	four
T2386	7733-7741	JJ	denotes	distinct
T2387	7742-7755	NN	denotes	transmembrane
T2385	7756-7763	NNS	denotes	domains
T2388	7764-7765	-LRB-	denotes	(
T2389	7765-7769	NN	denotes	Fig.
T2390	7770-7771	CD	denotes	5
T2391	7771-7772	-RRB-	denotes	)
T2392	7772-7774	,	denotes	,
T2393	7774-7777	CD	denotes	two
T2395	7778-7781	NN	denotes	CBS
T2394	7782-7789	NNS	denotes	domains
T2396	7790-7793	CC	denotes	and
T2397	7794-7795	DT	denotes	a
T2399	7796-7801	NN	denotes	DUF21
T2398	7802-7808	NN	denotes	domain
T2400	7809-7813	WDT	denotes	that
T2402	7814-7817	VBP	denotes	are
T2401	7818-7823	VBN	denotes	found
T2403	7824-7826	IN	denotes	in
T2404	7827-7835	NNS	denotes	bacteria
T2405	7836-7840	NN	denotes	CorC
T2407	7841-7844	CC	denotes	and
T2408	7845-7850	NN	denotes	yeast
T2409	7851-7856	NN	denotes	Amip3
T2406	7857-7865	NN	denotes	proteins
T2410	7865-7866	.	denotes	.
T2411	7866-7970	sentence	denotes	CBS domains are small intracellular modules that are mostly found in 2 or four copies within a protein.
T2412	7867-7870	NN	denotes	CBS
T2413	7871-7878	NNS	denotes	domains
T2414	7879-7882	VBP	denotes	are
T2415	7883-7888	JJ	denotes	small
T2417	7889-7902	JJ	denotes	intracellular
T2416	7903-7910	NNS	denotes	modules
T2418	7911-7915	WDT	denotes	that
T2420	7916-7919	VBP	denotes	are
T2421	7920-7926	RB	denotes	mostly
T2419	7927-7932	VBN	denotes	found
T2422	7933-7935	IN	denotes	in
T2423	7936-7937	CD	denotes	2
T2425	7938-7940	CC	denotes	or
T2426	7941-7945	CD	denotes	four
T2424	7946-7952	NNS	denotes	copies
T2427	7953-7959	IN	denotes	within
T2428	7960-7961	DT	denotes	a
T2429	7962-7969	NN	denotes	protein
T2430	7969-7970	.	denotes	.
T2431	7970-8038	sentence	denotes	Pairs of CBS domains dimerise to form a stable globular domain [8].
T2432	7971-7976	NNS	denotes	Pairs
T2434	7977-7979	IN	denotes	of
T2435	7980-7983	NN	denotes	CBS
T2436	7984-7991	NNS	denotes	domains
T2433	7992-8000	VBP	denotes	dimerise
T2437	8001-8003	TO	denotes	to
T2438	8004-8008	VB	denotes	form
T2439	8009-8010	DT	denotes	a
T2441	8011-8017	JJ	denotes	stable
T2442	8018-8026	JJ	denotes	globular
T2440	8027-8033	NN	denotes	domain
T2443	8034-8035	-LRB-	denotes	[
T2444	8035-8036	CD	denotes	8
T2445	8036-8037	-RRB-	denotes	]
T2446	8037-8038	.	denotes	.
T2447	8038-8111	sentence	denotes	DUF21 (CD: pfam01959.9) is a newly defined domain with unknown function.
T2448	8039-8044	NN	denotes	DUF21
T2450	8045-8046	-LRB-	denotes	(
T2452	8046-8048	NN	denotes	CD
T2453	8048-8050	:	denotes	:
T2451	8050-8061	NN	denotes	pfam01959.9
T2454	8061-8062	-RRB-	denotes	)
T2449	8063-8065	VBZ	denotes	is
T2455	8066-8067	DT	denotes	a
T2457	8068-8073	RB	denotes	newly
T2458	8074-8081	VBN	denotes	defined
T2456	8082-8088	NN	denotes	domain
T2459	8089-8093	IN	denotes	with
T2460	8094-8101	JJ	denotes	unknown
T2461	8102-8110	NN	denotes	function
T2462	8110-8111	.	denotes	.
T2463	8111-8251	sentence	denotes	This domain is a transmembrane region and found to be located in the N-terminus of the proteins adjacent to two intracellular CBS domains .
T2464	8112-8116	DT	denotes	This
T2465	8117-8123	NN	denotes	domain
T2466	8124-8126	VBZ	denotes	is
T2467	8127-8128	DT	denotes	a
T2469	8129-8142	JJ	denotes	transmembrane
T2468	8143-8149	NN	denotes	region
T2470	8150-8153	CC	denotes	and
T2471	8154-8159	VBN	denotes	found
T2472	8160-8162	TO	denotes	to
T2474	8163-8165	VB	denotes	be
T2473	8166-8173	VBN	denotes	located
T2475	8174-8176	IN	denotes	in
T2476	8177-8180	DT	denotes	the
T2478	8181-8182	NN	denotes	N
T2479	8182-8183	HYPH	denotes	-
T2477	8183-8191	NN	denotes	terminus
T2480	8192-8194	IN	denotes	of
T2481	8195-8198	DT	denotes	the
T2482	8199-8207	NN	denotes	proteins
T2483	8208-8216	JJ	denotes	adjacent
T2484	8217-8219	IN	denotes	to
T2485	8220-8223	CD	denotes	two
T2487	8224-8237	JJ	denotes	intracellular
T2488	8238-8241	NN	denotes	CBS
T2486	8242-8249	NNS	denotes	domains
T2489	8250-8251	.	denotes	.
T2490	8251-8353	sentence	denotes	A cNMP-binding domain (cyclic nucleotide-monophosphate-binding domain) was found in all Acdp members.
T2491	8252-8253	DT	denotes	A
T2493	8254-8258	NN	denotes	cNMP
T2495	8258-8259	HYPH	denotes	-
T2494	8259-8266	VBG	denotes	binding
T2492	8267-8273	NN	denotes	domain
T2497	8274-8275	-LRB-	denotes	(
T2499	8275-8281	JJ	denotes	cyclic
T2501	8282-8292	NN	denotes	nucleotide
T2502	8292-8293	HYPH	denotes	-
T2500	8293-8306	NN	denotes	monophosphate
T2503	8306-8307	HYPH	denotes	-
T2504	8307-8314	VBG	denotes	binding
T2498	8315-8321	NN	denotes	domain
T2505	8321-8322	-RRB-	denotes	)
T2506	8323-8326	VBD	denotes	was
T2496	8327-8332	VBN	denotes	found
T2507	8333-8335	IN	denotes	in
T2508	8336-8339	DT	denotes	all
T2510	8340-8344	NN	denotes	Acdp
T2509	8345-8352	NNS	denotes	members
T2511	8352-8353	.	denotes	.
T2512	8353-9080	sentence	denotes	Figure 5 Four transmembrane domains within Acdp4 protein. Transmembrane domains were predicted by the TMHMM program . The plot shows the posterior probabilities of inside /outside/TM helix. At the top of the plot (between 1 and 1.2) the N-best prediction is shown. The plot is obtained by calculating the total probability that a residue sits in helix, inside, or outside summed over all possible paths through the model. In addition, Acdp1 contains an Alanine-rich region (2–10: AAAAAAAAA), a Leucine-rich region (204–257: LLRVRPRLYGPGGDLLPPAWLRALGALLLLALSALF SGLRLSLLSLDPVELRVL), a Proline-rich region (78–130: PGPPVPAAPVPAPSLA PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP), and two amidation sites (917–920: MGKK; 926–929: SGRK).
T6228	8364-8368	CD	denotes	Four
T6230	8369-8382	NN	denotes	transmembrane
T6229	8383-8390	NNS	denotes	domains
T6231	8391-8397	IN	denotes	within
T6232	8398-8403	NN	denotes	Acdp4
T6233	8404-8411	NN	denotes	protein
T6234	8411-8412	.	denotes	.
T6235	8412-8472	sentence	denotes	Transmembrane domains were predicted by the TMHMM program .
T6236	8413-8426	NN	denotes	Transmembrane
T6237	8427-8434	NNS	denotes	domains
T6239	8435-8439	VBD	denotes	were
T6238	8440-8449	VBN	denotes	predicted
T6240	8450-8452	IN	denotes	by
T6241	8453-8456	DT	denotes	the
T6243	8457-8462	NN	denotes	TMHMM
T6242	8463-8470	NN	denotes	program
T6244	8471-8472	.	denotes	.
T6245	8472-8544	sentence	denotes	The plot shows the posterior probabilities of inside /outside/TM helix.
T6246	8473-8476	DT	denotes	The
T6247	8477-8481	NN	denotes	plot
T6248	8482-8487	VBZ	denotes	shows
T6249	8488-8491	DT	denotes	the
T6251	8492-8501	JJ	denotes	posterior
T6250	8502-8515	NNS	denotes	probabilities
T6252	8516-8518	IN	denotes	of
T6253	8519-8525	JJ	denotes	inside
T6255	8526-8527	HYPH	denotes	/
T6256	8527-8534	JJ	denotes	outside
T6257	8534-8535	HYPH	denotes	/
T6254	8535-8537	NN	denotes	TM
T6258	8538-8543	NN	denotes	helix
T6259	8543-8544	.	denotes	.
T6260	8544-8619	sentence	denotes	At the top of the plot (between 1 and 1.2) the N-best prediction is shown.
T6261	8545-8547	IN	denotes	At
T6263	8548-8551	DT	denotes	the
T6264	8552-8555	NN	denotes	top
T6265	8556-8558	IN	denotes	of
T6266	8559-8562	DT	denotes	the
T6267	8563-8567	NN	denotes	plot
T6268	8568-8569	-LRB-	denotes	(
T6269	8569-8576	IN	denotes	between
T6270	8577-8578	CD	denotes	1
T6271	8579-8582	CC	denotes	and
T6272	8583-8586	CD	denotes	1.2
T6273	8586-8587	-RRB-	denotes	)
T6274	8588-8591	DT	denotes	the
T6276	8592-8593	NN	denotes	N
T6278	8593-8594	HYPH	denotes	-
T6277	8594-8598	JJS	denotes	best
T6275	8599-8609	NN	denotes	prediction
T6279	8610-8612	VBZ	denotes	is
T6262	8613-8618	VBN	denotes	shown
T6280	8618-8619	.	denotes	.
T6281	8619-8776	sentence	denotes	The plot is obtained by calculating the total probability that a residue sits in helix, inside, or outside summed over all possible paths through the model.
T6282	8620-8623	DT	denotes	The
T6283	8624-8628	NN	denotes	plot
T6285	8629-8631	VBZ	denotes	is
T6284	8632-8640	VBN	denotes	obtained
T6286	8641-8643	IN	denotes	by
T6287	8644-8655	VBG	denotes	calculating
T6288	8656-8659	DT	denotes	the
T6290	8660-8665	JJ	denotes	total
T6289	8666-8677	NN	denotes	probability
T6291	8678-8682	IN	denotes	that
T6293	8683-8684	DT	denotes	a
T6294	8685-8692	NN	denotes	residue
T6292	8693-8697	VBZ	denotes	sits
T6295	8698-8700	IN	denotes	in
T6296	8701-8706	NN	denotes	helix
T6297	8706-8708	,	denotes	,
T6298	8708-8714	RB	denotes	inside
T6299	8714-8716	,	denotes	,
T6300	8716-8718	CC	denotes	or
T6301	8719-8726	RB	denotes	outside
T6302	8727-8733	VBD	denotes	summed
T6303	8734-8738	IN	denotes	over
T6304	8739-8742	DT	denotes	all
T6306	8743-8751	JJ	denotes	possible
T6305	8752-8757	NNS	denotes	paths
T6307	8758-8765	IN	denotes	through
T6308	8766-8769	DT	denotes	the
T6309	8770-8775	NN	denotes	model
T6310	8775-8776	.	denotes	.
T2513	8777-8779	IN	denotes	In
T2515	8780-8788	NN	denotes	addition
T2516	8788-8790	,	denotes	,
T2517	8790-8795	NN	denotes	Acdp1
T2514	8796-8804	VBZ	denotes	contains
T2518	8805-8807	DT	denotes	an
T2520	8808-8815	NN	denotes	Alanine
T2522	8815-8816	HYPH	denotes	-
T2521	8816-8820	JJ	denotes	rich
T2519	8821-8827	NN	denotes	region
T2523	8828-8829	-LRB-	denotes	(
T2525	8829-8830	CD	denotes	2
T2526	8830-8831	SYM	denotes	–
T2527	8831-8833	CD	denotes	10
T2528	8833-8835	:	denotes	:
T2524	8835-8844	NN	denotes	AAAAAAAAA
T2529	8844-8845	-RRB-	denotes	)
T2530	8845-8847	,	denotes	,
T2531	8847-8848	DT	denotes	a
T2533	8849-8856	NN	denotes	Leucine
T2535	8856-8857	HYPH	denotes	-
T2534	8857-8861	JJ	denotes	rich
T2532	8862-8868	NN	denotes	region
T2536	8869-8870	-LRB-	denotes	(
T2538	8870-8873	CD	denotes	204
T2539	8873-8874	SYM	denotes	–
T2540	8874-8877	CD	denotes	257
T2541	8877-8879	:	denotes	:
T2542	8879-8915	NN	denotes	LLRVRPRLYGPGGDLLPPAWLRALGALLLLALSALF
T2537	8916-8934	NN	denotes	SGLRLSLLSLDPVELRVL
T2543	8934-8935	-RRB-	denotes	)
T2544	8935-8937	,	denotes	,
T2545	8937-8938	DT	denotes	a
T2547	8939-8946	NN	denotes	Proline
T2549	8946-8947	HYPH	denotes	-
T2548	8947-8951	JJ	denotes	rich
T2546	8952-8958	NN	denotes	region
T2550	8959-8960	-LRB-	denotes	(
T2552	8960-8962	CD	denotes	78
T2553	8962-8963	SYM	denotes	–
T2554	8963-8966	CD	denotes	130
T2555	8966-8968	:	denotes	:
T2556	8968-8984	NN	denotes	PGPPVPAAPVPAPSLA
T2551	8985-9022	NN	denotes	PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
T2557	9022-9023	-RRB-	denotes	)
T2558	9023-9025	,	denotes	,
T2559	9025-9028	CC	denotes	and
T2560	9029-9032	CD	denotes	two
T2562	9033-9042	NN	denotes	amidation
T2561	9043-9048	NNS	denotes	sites
T2563	9049-9050	-LRB-	denotes	(
T2565	9050-9053	CD	denotes	917
T2567	9053-9054	SYM	denotes	–
T2568	9054-9057	CD	denotes	920
T2569	9057-9059	:	denotes	:
T2566	9059-9063	NN	denotes	MGKK
T2570	9063-9064	:	denotes	;
T2571	9065-9068	CD	denotes	926
T2572	9068-9069	SYM	denotes	–
T2573	9069-9072	CD	denotes	929
T2574	9072-9074	:	denotes	:
T2564	9074-9078	NN	denotes	SGRK
T2575	9078-9079	-RRB-	denotes	)
T2576	9079-9080	.	denotes	.
T2577	9080-9147	sentence	denotes	Acdp2 has a glycine-rich region (201–222: GAGGSGSASGTVGGKGGAGVAG).
T2578	9081-9086	NN	denotes	Acdp2
T2579	9087-9090	VBZ	denotes	has
T2580	9091-9092	DT	denotes	a
T2582	9093-9100	NN	denotes	glycine
T2584	9100-9101	HYPH	denotes	-
T2583	9101-9105	JJ	denotes	rich
T2581	9106-9112	NN	denotes	region
T2585	9113-9114	-LRB-	denotes	(
T2587	9114-9117	CD	denotes	201
T2588	9117-9118	SYM	denotes	–
T2589	9118-9121	CD	denotes	222
T2590	9121-9123	:	denotes	:
T2586	9123-9145	NN	denotes	GAGGSGSASGTVGGKGGAGVAG
T2591	9145-9146	-RRB-	denotes	)
T2592	9146-9147	.	denotes	.
T2593	9147-9242	sentence	denotes	Acdp3 possesses a large alanine-rich region (2–261) and a large leucine-rich region (201–299).
T2594	9148-9153	NN	denotes	Acdp3
T2595	9154-9163	VBZ	denotes	possesses
T2596	9164-9165	DT	denotes	a
T2598	9166-9171	JJ	denotes	large
T2599	9172-9179	NN	denotes	alanine
T2601	9179-9180	HYPH	denotes	-
T2600	9180-9184	JJ	denotes	rich
T2597	9185-9191	NN	denotes	region
T2602	9192-9193	-LRB-	denotes	(
T2603	9193-9194	CD	denotes	2
T2604	9194-9195	SYM	denotes	–
T2605	9195-9198	CD	denotes	261
T2606	9198-9199	-RRB-	denotes	)
T2607	9200-9203	CC	denotes	and
T2608	9204-9205	DT	denotes	a
T2610	9206-9211	JJ	denotes	large
T2611	9212-9219	NN	denotes	leucine
T2613	9219-9220	HYPH	denotes	-
T2612	9220-9224	JJ	denotes	rich
T2609	9225-9231	NN	denotes	region
T2614	9232-9233	-LRB-	denotes	(
T2615	9233-9236	CD	denotes	201
T2616	9236-9237	SYM	denotes	–
T2617	9237-9240	CD	denotes	299
T2618	9240-9241	-RRB-	denotes	)
T2619	9241-9242	.	denotes	.
T2620	9242-9352	sentence	denotes	Acdp4 contains a leucine zipper pattern (185–206: LVMVLLVLSGIFSGLNLGLMAL) and an amidation site (7–10: GGRR).
T2621	9243-9248	NN	denotes	Acdp4
T2622	9249-9257	VBZ	denotes	contains
T2623	9258-9259	DT	denotes	a
T2625	9260-9267	NN	denotes	leucine
T2626	9268-9274	NN	denotes	zipper
T2624	9275-9282	NN	denotes	pattern
T2627	9283-9284	-LRB-	denotes	(
T2629	9284-9287	CD	denotes	185
T2630	9287-9288	SYM	denotes	–
T2631	9288-9291	CD	denotes	206
T2632	9291-9293	:	denotes	:
T2628	9293-9315	NN	denotes	LVMVLLVLSGIFSGLNLGLMAL
T2633	9315-9316	-RRB-	denotes	)
T2634	9317-9320	CC	denotes	and
T2635	9321-9323	DT	denotes	an
T2637	9324-9333	NN	denotes	amidation
T2636	9334-9338	NN	denotes	site
T2638	9339-9340	-LRB-	denotes	(
T2640	9340-9341	CD	denotes	7
T2641	9341-9342	SYM	denotes	–
T2642	9342-9344	CD	denotes	10
T2643	9344-9346	:	denotes	:
T2639	9346-9350	NN	denotes	GGRR
T2644	9350-9351	-RRB-	denotes	)
T2645	9351-9352	.	denotes	.
T2862	9354-9362	NN	denotes	Antibody
T2863	9363-9373	NN	denotes	production
T2864	9373-9375	,	denotes	,
T2865	9375-9382	NNP	denotes	Western
T2866	9383-9390	NNS	denotes	results
T2867	9391-9394	CC	denotes	and
T2868	9395-9406	JJ	denotes	subcellular
T2869	9407-9419	NN	denotes	localization
T2870	9419-9627	sentence	denotes	Peptides from Acdp1 N- (TSFLLRVYFQPGPPATAAPVPSPT) and C- (TQQLTLSPAAVPTR) terminuses, conserved peptide from ACD domain of Acdp1 (HNIVDILFVKDLAFVDPDDCTPLLTVTRF) were commercially synthesized (Sigma Genosys).
T2871	9420-9428	NNS	denotes	Peptides
T2873	9429-9433	IN	denotes	from
T2874	9434-9439	NN	denotes	Acdp1
T2876	9440-9441	NN	denotes	N
T2877	9441-9442	HYPH	denotes	-
T2878	9443-9444	-LRB-	denotes	(
T2879	9444-9468	NN	denotes	TSFLLRVYFQPGPPATAAPVPSPT
T2880	9468-9469	-RRB-	denotes	)
T2881	9470-9473	CC	denotes	and
T2882	9474-9475	NN	denotes	C
T2883	9475-9476	HYPH	denotes	-
T2884	9477-9478	-LRB-	denotes	(
T2885	9478-9492	NN	denotes	TQQLTLSPAAVPTR
T2886	9492-9493	-RRB-	denotes	)
T2875	9494-9504	NNS	denotes	terminuses
T2887	9504-9506	,	denotes	,
T2888	9506-9515	JJ	denotes	conserved
T2889	9516-9523	NN	denotes	peptide
T2890	9524-9528	IN	denotes	from
T2891	9529-9532	NN	denotes	ACD
T2892	9533-9539	NN	denotes	domain
T2893	9540-9542	IN	denotes	of
T2894	9543-9548	NN	denotes	Acdp1
T2895	9549-9550	-LRB-	denotes	(
T2896	9550-9579	NN	denotes	HNIVDILFVKDLAFVDPDDCTPLLTVTRF
T2897	9579-9580	-RRB-	denotes	)
T2898	9581-9585	VBD	denotes	were
T2899	9586-9598	RB	denotes	commercially
T2872	9599-9610	VBN	denotes	synthesized
T2900	9611-9612	-LRB-	denotes	(
T2902	9612-9617	NNP	denotes	Sigma
T2901	9618-9625	NNP	denotes	Genosys
T2903	9625-9626	-RRB-	denotes	)
T2904	9626-9627	.	denotes	.
T2905	9627-9775	sentence	denotes	These antigenic sites were predicted by software from Sigma Genosys and polyclonal antibodies for each peptide were produced by immunizing rabbits.
T2906	9628-9633	DT	denotes	These
T2908	9634-9643	JJ	denotes	antigenic
T2907	9644-9649	NNS	denotes	sites
T2910	9650-9654	VBD	denotes	were
T2909	9655-9664	VBN	denotes	predicted
T2911	9665-9667	IN	denotes	by
T2912	9668-9676	NN	denotes	software
T2913	9677-9681	IN	denotes	from
T2914	9682-9687	NNP	denotes	Sigma
T2915	9688-9695	NNP	denotes	Genosys
T2916	9696-9699	CC	denotes	and
T2917	9700-9710	JJ	denotes	polyclonal
T2918	9711-9721	NNS	denotes	antibodies
T2920	9722-9725	IN	denotes	for
T2921	9726-9730	DT	denotes	each
T2922	9731-9738	NN	denotes	peptide
T2923	9739-9743	VBD	denotes	were
T2919	9744-9752	VBN	denotes	produced
T2924	9753-9755	IN	denotes	by
T2925	9756-9766	VBG	denotes	immunizing
T2926	9767-9774	NNS	denotes	rabbits
T2927	9774-9775	.	denotes	.
T2928	9775-9885	sentence	denotes	To test the specificity of the antibodies, we conducted Western-blot analysis of mouse brain tissue extracts.
T2929	9776-9778	TO	denotes	To
T2930	9779-9783	VB	denotes	test
T2932	9784-9787	DT	denotes	the
T2933	9788-9799	NN	denotes	specificity
T2934	9800-9802	IN	denotes	of
T2935	9803-9806	DT	denotes	the
T2936	9807-9817	NNS	denotes	antibodies
T2937	9817-9819	,	denotes	,
T2938	9819-9821	PRP	denotes	we
T2931	9822-9831	VBD	denotes	conducted
T2939	9832-9839	NNP	denotes	Western
T2941	9839-9840	HYPH	denotes	-
T2940	9840-9844	NN	denotes	blot
T2942	9845-9853	NN	denotes	analysis
T2943	9854-9856	IN	denotes	of
T2944	9857-9862	NN	denotes	mouse
T2945	9863-9868	NN	denotes	brain
T2946	9869-9875	NN	denotes	tissue
T2947	9876-9884	NNS	denotes	extracts
T2948	9884-9885	.	denotes	.
T2949	9885-9988	sentence	denotes	As shown in Fig. 6A, the antibody produced by C-terminal peptide specifically detected Acdp1 (lane 3).
T2950	9886-9888	IN	denotes	As
T2951	9889-9894	VBN	denotes	shown
T2953	9895-9897	IN	denotes	in
T2954	9898-9902	NN	denotes	Fig.
T2955	9903-9905	CD	denotes	6A
T2956	9905-9907	,	denotes	,
T2957	9907-9910	DT	denotes	the
T2958	9911-9919	NN	denotes	antibody
T2959	9920-9928	VBN	denotes	produced
T2960	9929-9931	IN	denotes	by
T2961	9932-9933	NN	denotes	C
T2963	9933-9934	HYPH	denotes	-
T2964	9934-9942	JJ	denotes	terminal
T2962	9943-9950	NN	denotes	peptide
T2965	9951-9963	RB	denotes	specifically
T2952	9964-9972	VBD	denotes	detected
T2966	9973-9978	NN	denotes	Acdp1
T2967	9979-9980	-LRB-	denotes	(
T2968	9980-9984	NN	denotes	lane
T2969	9985-9986	CD	denotes	3
T2970	9986-9987	-RRB-	denotes	)
T2971	9987-9988	.	denotes	.
T2972	9988-10150	sentence	denotes	The antibody generated by N-terminal peptide recognized Acdp4 in addition to Acdp1, although the reactivity to latter was significantly higher (Fig. 6A, lane 2).
T2973	9989-9992	DT	denotes	The
T2974	9993-10001	NN	denotes	antibody
T2976	10002-10011	VBN	denotes	generated
T2977	10012-10014	IN	denotes	by
T2978	10015-10016	NN	denotes	N
T2980	10016-10017	HYPH	denotes	-
T2981	10017-10025	JJ	denotes	terminal
T2979	10026-10033	NN	denotes	peptide
T2975	10034-10044	VBD	denotes	recognized
T2982	10045-10050	NN	denotes	Acdp4
T2983	10051-10053	IN	denotes	in
T2984	10054-10062	NN	denotes	addition
T2985	10063-10065	IN	denotes	to
T2986	10066-10071	NN	denotes	Acdp1
T2987	10071-10073	,	denotes	,
T2988	10073-10081	IN	denotes	although
T2990	10082-10085	DT	denotes	the
T2991	10086-10096	NN	denotes	reactivity
T2992	10097-10099	IN	denotes	to
T2993	10100-10106	NN	denotes	latter
T2989	10107-10110	VBD	denotes	was
T2994	10111-10124	RB	denotes	significantly
T2995	10125-10131	JJR	denotes	higher
T2996	10132-10133	-LRB-	denotes	(
T2998	10133-10137	NN	denotes	Fig.
T2999	10138-10140	CD	denotes	6A
T3000	10140-10142	,	denotes	,
T2997	10142-10146	NN	denotes	lane
T3001	10147-10148	CD	denotes	2
T3002	10148-10149	-RRB-	denotes	)
T3003	10149-10150	.	denotes	.
T3004	10150-10265	sentence	denotes	As expected, the antibody produced by the conserved sequence peptide detected all Acdp proteins (Fig. 6A, lane 1).
T3005	10151-10153	IN	denotes	As
T3006	10154-10162	VBN	denotes	expected
T3008	10162-10164	,	denotes	,
T3009	10164-10167	DT	denotes	the
T3010	10168-10176	NN	denotes	antibody
T3011	10177-10185	VBN	denotes	produced
T3012	10186-10188	IN	denotes	by
T3013	10189-10192	DT	denotes	the
T3015	10193-10202	VBN	denotes	conserved
T3016	10203-10211	NN	denotes	sequence
T3014	10212-10219	NN	denotes	peptide
T3007	10220-10228	VBD	denotes	detected
T3017	10229-10232	DT	denotes	all
T3019	10233-10237	NN	denotes	Acdp
T3018	10238-10246	NN	denotes	proteins
T3020	10247-10248	-LRB-	denotes	(
T3022	10248-10252	NN	denotes	Fig.
T3023	10253-10255	CD	denotes	6A
T3024	10255-10257	,	denotes	,
T3021	10257-10261	NN	denotes	lane
T3025	10262-10263	CD	denotes	1
T3026	10263-10264	-RRB-	denotes	)
T3027	10264-10265	.	denotes	.
T3028	10265-10400	sentence	denotes	To further determine the specificity of the antibody against the Acdp1 C-terminus, we analyzed extracts of HEK293, 3T3 and PC12 cells.
T3029	10266-10268	TO	denotes	To
T3031	10269-10276	RB	denotes	further
T3030	10277-10286	VB	denotes	determine
T3033	10287-10290	DT	denotes	the
T3034	10291-10302	NN	denotes	specificity
T3035	10303-10305	IN	denotes	of
T3036	10306-10309	DT	denotes	the
T3037	10310-10318	NN	denotes	antibody
T3038	10319-10326	IN	denotes	against
T3039	10327-10330	DT	denotes	the
T3041	10331-10336	NN	denotes	Acdp1
T3042	10337-10338	NN	denotes	C
T3043	10338-10339	HYPH	denotes	-
T3040	10339-10347	NN	denotes	terminus
T3044	10347-10349	,	denotes	,
T3045	10349-10351	PRP	denotes	we
T3032	10352-10360	VBD	denotes	analyzed
T3046	10361-10369	NNS	denotes	extracts
T3047	10370-10372	IN	denotes	of
T3048	10373-10379	NN	denotes	HEK293
T3050	10379-10381	,	denotes	,
T3051	10381-10384	NN	denotes	3T3
T3052	10385-10388	CC	denotes	and
T3053	10389-10393	NN	denotes	PC12
T3049	10394-10399	NNS	denotes	cells
T3054	10399-10400	.	denotes	.
T3055	10400-10434	sentence	denotes	The results are shown in Fig. 6B.
T3056	10401-10404	DT	denotes	The
T3057	10405-10412	NNS	denotes	results
T3059	10413-10416	VBP	denotes	are
T3058	10417-10422	VBN	denotes	shown
T3060	10423-10425	IN	denotes	in
T3061	10426-10430	NN	denotes	Fig.
T3062	10431-10433	CD	denotes	6B
T3063	10433-10434	.	denotes	.
T3064	10434-10515	sentence	denotes	Apparently, this antibody detected a specific band of Acdp1 in all cell lysates.
T3065	10435-10445	RB	denotes	Apparently
T3067	10445-10447	,	denotes	,
T3068	10447-10451	DT	denotes	this
T3069	10452-10460	NN	denotes	antibody
T3066	10461-10469	VBD	denotes	detected
T3070	10470-10471	DT	denotes	a
T3072	10472-10480	JJ	denotes	specific
T3071	10481-10485	NN	denotes	band
T3073	10486-10488	IN	denotes	of
T3074	10489-10494	NN	denotes	Acdp1
T3075	10495-10497	IN	denotes	in
T3076	10498-10501	DT	denotes	all
T3078	10502-10506	NN	denotes	cell
T3077	10507-10514	NNS	denotes	lysates
T3079	10514-10515	.	denotes	.
T3080	10515-10641	sentence	denotes	Of note, shown in Fig. 6B are signals of 10 μg extracts of HEK293 cell lysates, 100 μg extracts of 3T3 and PC12 cell lysates.
T3081	10516-10518	IN	denotes	Of
T3083	10519-10523	NN	denotes	note
T3084	10523-10525	,	denotes	,
T3085	10525-10530	VBN	denotes	shown
T3086	10531-10533	IN	denotes	in
T3087	10534-10538	NN	denotes	Fig.
T3088	10539-10541	CD	denotes	6B
T3082	10542-10545	VBP	denotes	are
T3089	10546-10553	NNS	denotes	signals
T3090	10554-10556	IN	denotes	of
T3091	10557-10559	CD	denotes	10
T3092	10560-10562	NN	denotes	μg
T3093	10563-10571	NNS	denotes	extracts
T3094	10572-10574	IN	denotes	of
T3095	10575-10581	NN	denotes	HEK293
T3097	10582-10586	NN	denotes	cell
T3096	10587-10594	NNS	denotes	lysates
T3098	10594-10596	,	denotes	,
T3099	10596-10599	CD	denotes	100
T3100	10600-10602	NN	denotes	μg
T3101	10603-10611	NNS	denotes	extracts
T3102	10612-10614	IN	denotes	of
T3103	10615-10618	NN	denotes	3T3
T3105	10619-10622	CC	denotes	and
T3106	10623-10627	NN	denotes	PC12
T3107	10628-10632	NN	denotes	cell
T3104	10633-10640	NNS	denotes	lysates
T3108	10640-10641	.	denotes	.
T3109	10641-10755	sentence	denotes	Thus, the expression levels of Acdp1 in these cell types vary a lot, with the highest expression in HEK293 cells.
T3110	10642-10646	RB	denotes	Thus
T3112	10646-10648	,	denotes	,
T3113	10648-10651	DT	denotes	the
T3115	10652-10662	NN	denotes	expression
T3114	10663-10669	NNS	denotes	levels
T3116	10670-10672	IN	denotes	of
T3117	10673-10678	NN	denotes	Acdp1
T3118	10679-10681	IN	denotes	in
T3119	10682-10687	DT	denotes	these
T3121	10688-10692	NN	denotes	cell
T3120	10693-10698	NNS	denotes	types
T3111	10699-10703	VBP	denotes	vary
T3122	10704-10705	DT	denotes	a
T3123	10706-10709	NN	denotes	lot
T3124	10709-10711	,	denotes	,
T3125	10711-10715	IN	denotes	with
T3126	10716-10719	DT	denotes	the
T3128	10720-10727	JJS	denotes	highest
T3127	10728-10738	NN	denotes	expression
T3129	10739-10741	IN	denotes	in
T3130	10742-10748	NN	denotes	HEK293
T3131	10749-10754	NNS	denotes	cells
T3132	10754-10755	.	denotes	.
T3133	10755-10915	sentence	denotes	Nevertheless, these immunoblot results support our analysis of brain tissue extracts that the antibody against Acdp1 C-terminus specifically recognizes Acdp-1.
T3134	10756-10768	RB	denotes	Nevertheless
T3136	10768-10770	,	denotes	,
T3137	10770-10775	DT	denotes	these
T3139	10776-10786	NN	denotes	immunoblot
T3138	10787-10794	NNS	denotes	results
T3135	10795-10802	VBP	denotes	support
T3140	10803-10806	PRP$	denotes	our
T3141	10807-10815	NN	denotes	analysis
T3142	10816-10818	IN	denotes	of
T3143	10819-10824	NN	denotes	brain
T3145	10825-10831	NN	denotes	tissue
T3144	10832-10840	NNS	denotes	extracts
T3146	10841-10845	IN	denotes	that
T3148	10846-10849	DT	denotes	the
T3149	10850-10858	NN	denotes	antibody
T3150	10859-10866	IN	denotes	against
T3151	10867-10872	NN	denotes	Acdp1
T3153	10873-10874	NN	denotes	C
T3154	10874-10875	HYPH	denotes	-
T3152	10875-10883	NN	denotes	terminus
T3155	10884-10896	RB	denotes	specifically
T3147	10897-10907	VBZ	denotes	recognizes
T3156	10908-10912	NN	denotes	Acdp
T3157	10912-10913	HYPH	denotes	-
T3158	10913-10914	CD	denotes	1
T3159	10914-10915	.	denotes	.
T3160	10915-11034	sentence	denotes	The specificity of the Acdp1 C-terminus antibody suggests the possibility of using it to localize Acdp-1 within cells.
T3161	10916-10919	DT	denotes	The
T3162	10920-10931	NN	denotes	specificity
T3164	10932-10934	IN	denotes	of
T3165	10935-10938	DT	denotes	the
T3167	10939-10944	NN	denotes	Acdp1
T3168	10945-10946	NN	denotes	C
T3170	10946-10947	HYPH	denotes	-
T3169	10947-10955	NN	denotes	terminus
T3166	10956-10964	NN	denotes	antibody
T3163	10965-10973	VBZ	denotes	suggests
T3171	10974-10977	DT	denotes	the
T3172	10978-10989	NN	denotes	possibility
T3173	10990-10992	IN	denotes	of
T3174	10993-10998	VBG	denotes	using
T3175	10999-11001	PRP	denotes	it
T3176	11002-11004	TO	denotes	to
T3177	11005-11013	VB	denotes	localize
T3178	11014-11018	NN	denotes	Acdp
T3179	11018-11019	HYPH	denotes	-
T3180	11019-11020	CD	denotes	1
T3181	11021-11027	IN	denotes	within
T3182	11028-11033	NNS	denotes	cells
T3183	11033-11034	.	denotes	.
T3184	11034-11207	sentence	denotes	Since Northern blot revealed almost exclusive expression of Acdp1 in the brain, we examined its subcellular localization in hippocampus neurons isolated from mouse embryos.
T3185	11035-11040	IN	denotes	Since
T3187	11041-11049	NNP	denotes	Northern
T3188	11050-11054	NN	denotes	blot
T3186	11055-11063	VBD	denotes	revealed
T3190	11064-11070	RB	denotes	almost
T3191	11071-11080	JJ	denotes	exclusive
T3192	11081-11091	NN	denotes	expression
T3193	11092-11094	IN	denotes	of
T3194	11095-11100	NN	denotes	Acdp1
T3195	11101-11103	IN	denotes	in
T3196	11104-11107	DT	denotes	the
T3197	11108-11113	NN	denotes	brain
T3198	11113-11115	,	denotes	,
T3199	11115-11117	PRP	denotes	we
T3189	11118-11126	VBD	denotes	examined
T3200	11127-11130	PRP$	denotes	its
T3202	11131-11142	JJ	denotes	subcellular
T3201	11143-11155	NN	denotes	localization
T3203	11156-11158	IN	denotes	in
T3204	11159-11170	NN	denotes	hippocampus
T3205	11171-11178	NNS	denotes	neurons
T3206	11179-11187	VBN	denotes	isolated
T3207	11188-11192	IN	denotes	from
T3208	11193-11198	NN	denotes	mouse
T3209	11199-11206	NNS	denotes	embryos
T3210	11206-11207	.	denotes	.
T3211	11207-11327	sentence	denotes	The neurons were cultured on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes in dishes.
T3212	11208-11211	DT	denotes	The
T3213	11212-11219	NNS	denotes	neurons
T3215	11220-11224	VBD	denotes	were
T3214	11225-11233	VBN	denotes	cultured
T3216	11234-11236	IN	denotes	on
T3217	11237-11242	NN	denotes	glass
T3218	11243-11253	NNS	denotes	coverslips
T3219	11254-11260	VBN	denotes	coated
T3220	11261-11265	IN	denotes	with
T3221	11266-11267	DT	denotes	a
T3223	11268-11277	JJ	denotes	confluent
T3222	11278-11287	NN	denotes	monolayer
T3224	11288-11290	IN	denotes	of
T3225	11291-11296	NN	denotes	mouse
T3227	11297-11305	JJ	denotes	cortical
T3226	11306-11316	NNS	denotes	astrocytes
T3228	11317-11319	IN	denotes	in
T3229	11320-11326	NNS	denotes	dishes
T3230	11326-11327	.	denotes	.
T3231	11327-11392	sentence	denotes	Immunostaining was using the Acdp1 C-terminus specific antibody.
T3232	11328-11342	NN	denotes	Immunostaining
T3234	11343-11346	VBD	denotes	was
T3233	11347-11352	VBG	denotes	using
T3235	11353-11356	DT	denotes	the
T3237	11357-11362	NN	denotes	Acdp1
T3238	11363-11364	NN	denotes	C
T3240	11364-11365	HYPH	denotes	-
T3239	11365-11373	NN	denotes	terminus
T3241	11374-11382	JJ	denotes	specific
T3236	11383-11391	NN	denotes	antibody
T3242	11391-11392	.	denotes	.
T3243	11392-11480	sentence	denotes	Confocal imaging revealed that Acdp1 is predominantly localized on the plasma membrane.
T3244	11393-11401	JJ	denotes	Confocal
T3245	11402-11409	NN	denotes	imaging
T3246	11410-11418	VBD	denotes	revealed
T3247	11419-11423	IN	denotes	that
T3249	11424-11429	NN	denotes	Acdp1
T3250	11430-11432	VBZ	denotes	is
T3251	11433-11446	RB	denotes	predominantly
T3248	11447-11456	VBN	denotes	localized
T3252	11457-11459	IN	denotes	on
T3253	11460-11463	DT	denotes	the
T3255	11464-11470	NN	denotes	plasma
T3254	11471-11479	NN	denotes	membrane
T3256	11479-11480	.	denotes	.
T3257	11480-11707	sentence	denotes	A series of sections of a cell at the thickness of 0.5 micrometer clearly showed membrane location of Acdp1-immunoreactivity (Fig. 7), which is consistent with the observation of transmembrane domains within the Acdp proteins.
T3258	11481-11482	DT	denotes	A
T3259	11483-11489	NN	denotes	series
T3261	11490-11492	IN	denotes	of
T3262	11493-11501	NNS	denotes	sections
T3263	11502-11504	IN	denotes	of
T3264	11505-11506	DT	denotes	a
T3265	11507-11511	NN	denotes	cell
T3266	11512-11514	IN	denotes	at
T3267	11515-11518	DT	denotes	the
T3268	11519-11528	NN	denotes	thickness
T3269	11529-11531	IN	denotes	of
T3270	11532-11535	CD	denotes	0.5
T3271	11536-11546	NN	denotes	micrometer
T3272	11547-11554	RB	denotes	clearly
T3260	11555-11561	VBD	denotes	showed
T3273	11562-11570	NN	denotes	membrane
T3274	11571-11579	NN	denotes	location
T3275	11580-11582	IN	denotes	of
T3276	11583-11588	NN	denotes	Acdp1
T3278	11588-11589	HYPH	denotes	-
T3277	11589-11605	NN	denotes	immunoreactivity
T3279	11606-11607	-LRB-	denotes	(
T3280	11607-11611	NN	denotes	Fig.
T3281	11612-11613	CD	denotes	7
T3282	11613-11614	-RRB-	denotes	)
T3283	11614-11616	,	denotes	,
T3284	11616-11621	WDT	denotes	which
T3285	11622-11624	VBZ	denotes	is
T3286	11625-11635	JJ	denotes	consistent
T3287	11636-11640	IN	denotes	with
T3288	11641-11644	DT	denotes	the
T3289	11645-11656	NN	denotes	observation
T3290	11657-11659	IN	denotes	of
T3291	11660-11673	NN	denotes	transmembrane
T3292	11674-11681	NNS	denotes	domains
T3293	11682-11688	IN	denotes	within
T3294	11689-11692	DT	denotes	the
T3296	11693-11697	NN	denotes	Acdp
T3295	11698-11706	NN	denotes	proteins
T3297	11706-11707	.	denotes	.
T6368	11718-11722	NN	denotes	Fig.
T6370	11723-11725	CD	denotes	6A
T6371	11725-11727	:	denotes	:
T6372	11727-11737	NN	denotes	Immunoblot
T6369	11738-11746	NN	denotes	analysis
T6373	11747-11749	IN	denotes	of
T6374	11750-11754	NN	denotes	Acdp
T6375	11755-11763	NN	denotes	proteins
T6376	11764-11766	IN	denotes	in
T6377	11767-11772	NN	denotes	brain
T6378	11773-11779	NN	denotes	tissue
T6379	11780-11788	NNS	denotes	extracts
T6380	11788-11789	.	denotes	.
T6381	11789-11879	sentence	denotes	Immunoblotting were carried out using a Western blotting detection system (ECL) (PIERCE).
T6382	11790-11804	NN	denotes	Immunoblotting
T6384	11805-11809	VBD	denotes	were
T6383	11810-11817	VBN	denotes	carried
T6385	11818-11821	RP	denotes	out
T6386	11822-11827	VBG	denotes	using
T6387	11828-11829	DT	denotes	a
T6389	11830-11837	NNP	denotes	Western
T6390	11838-11846	NN	denotes	blotting
T6391	11847-11856	NN	denotes	detection
T6388	11857-11863	NN	denotes	system
T6392	11864-11865	-LRB-	denotes	(
T6393	11865-11868	NN	denotes	ECL
T6394	11868-11869	-RRB-	denotes	)
T6395	11870-11871	-LRB-	denotes	(
T6396	11871-11877	NN	denotes	PIERCE
T6397	11877-11878	-RRB-	denotes	)
T6398	11878-11879	.	denotes	.
T6399	11879-11940	sentence	denotes	Lane 1, probed with antibody generated by conserved peptide.
T6400	11880-11884	NN	denotes	Lane
T6402	11885-11886	CD	denotes	1
T6403	11886-11888	,	denotes	,
T6401	11888-11894	VBN	denotes	probed
T6404	11895-11899	IN	denotes	with
T6405	11900-11908	NN	denotes	antibody
T6406	11909-11918	VBN	denotes	generated
T6407	11919-11921	IN	denotes	by
T6408	11922-11931	JJ	denotes	conserved
T6409	11932-11939	NN	denotes	peptide
T6410	11939-11940	.	denotes	.
T6411	11940-12052	sentence	denotes	From top to bottom, each band corresponding to Acdp1 (115 kD), Acdp2 (100 kD), Acdp4 (90 kD) and Acdp3 (80 kD).
T6412	11941-11945	IN	denotes	From
T6414	11946-11949	NN	denotes	top
T6415	11950-11952	IN	denotes	to
T6416	11953-11959	NN	denotes	bottom
T6417	11959-11961	,	denotes	,
T6418	11961-11965	DT	denotes	each
T6413	11966-11970	NN	denotes	band
T6419	11971-11984	VBG	denotes	corresponding
T6420	11985-11987	IN	denotes	to
T6421	11988-11993	NN	denotes	Acdp1
T6422	11994-11995	-LRB-	denotes	(
T6424	11995-11998	CD	denotes	115
T6423	11999-12001	NN	denotes	kD
T6425	12001-12002	-RRB-	denotes	)
T6426	12002-12004	,	denotes	,
T6427	12004-12009	NN	denotes	Acdp2
T6428	12010-12011	-LRB-	denotes	(
T6430	12011-12014	CD	denotes	100
T6429	12015-12017	NN	denotes	kD
T6431	12017-12018	-RRB-	denotes	)
T6432	12018-12020	,	denotes	,
T6433	12020-12025	NN	denotes	Acdp4
T6434	12026-12027	-LRB-	denotes	(
T6436	12027-12029	CD	denotes	90
T6435	12030-12032	NN	denotes	kD
T6437	12032-12033	-RRB-	denotes	)
T6438	12034-12037	CC	denotes	and
T6439	12038-12043	NN	denotes	Acdp3
T6440	12044-12045	-LRB-	denotes	(
T6442	12045-12047	CD	denotes	80
T6441	12048-12050	NN	denotes	kD
T6443	12050-12051	-RRB-	denotes	)
T6444	12051-12052	.	denotes	.
T6445	12052-12162	sentence	denotes	Lane 2 and 3, probed with the Acdp1 antibodies generated by N-terminal and C-terminal peptides, respectively.
T6446	12053-12057	NN	denotes	Lane
T6447	12058-12059	CD	denotes	2
T6449	12060-12063	CC	denotes	and
T6450	12064-12065	CD	denotes	3
T6451	12065-12067	,	denotes	,
T6448	12067-12073	VBN	denotes	probed
T6452	12074-12078	IN	denotes	with
T6453	12079-12082	DT	denotes	the
T6455	12083-12088	NN	denotes	Acdp1
T6454	12089-12099	NNS	denotes	antibodies
T6456	12100-12109	VBN	denotes	generated
T6457	12110-12112	IN	denotes	by
T6458	12113-12114	NN	denotes	N
T6460	12114-12115	HYPH	denotes	-
T6459	12115-12123	JJ	denotes	terminal
T6462	12124-12127	CC	denotes	and
T6463	12128-12129	NN	denotes	C
T6465	12129-12130	HYPH	denotes	-
T6464	12130-12138	JJ	denotes	terminal
T6461	12139-12147	NNS	denotes	peptides
T6466	12147-12149	,	denotes	,
T6467	12149-12161	RB	denotes	respectively
T6468	12161-12162	.	denotes	.
T6469	12162-12231	sentence	denotes	Fig. 6B: Immunoblot analysis of Acdp1 in HEK293, 3T3 and PC12 cells.
T6470	12163-12167	NN	denotes	Fig.
T6472	12168-12170	CD	denotes	6B
T6473	12170-12172	:	denotes	:
T6474	12172-12182	NN	denotes	Immunoblot
T6471	12183-12191	NN	denotes	analysis
T6475	12192-12194	IN	denotes	of
T6476	12195-12200	NN	denotes	Acdp1
T6477	12201-12203	IN	denotes	in
T6478	12204-12210	NN	denotes	HEK293
T6480	12210-12212	,	denotes	,
T6481	12212-12215	NN	denotes	3T3
T6482	12216-12219	CC	denotes	and
T6483	12220-12224	NN	denotes	PC12
T6479	12225-12230	NNS	denotes	cells
T6484	12230-12231	.	denotes	.
T6485	12231-12305	sentence	denotes	The blots were probed with the antibody against the C-terminus of Acdp-1.
T6486	12232-12235	DT	denotes	The
T6487	12236-12241	NNS	denotes	blots
T6489	12242-12246	VBD	denotes	were
T6488	12247-12253	VBN	denotes	probed
T6490	12254-12258	IN	denotes	with
T6491	12259-12262	DT	denotes	the
T6492	12263-12271	NN	denotes	antibody
T6493	12272-12279	IN	denotes	against
T6494	12280-12283	DT	denotes	the
T6496	12284-12285	NN	denotes	C
T6497	12285-12286	HYPH	denotes	-
T6495	12286-12294	NN	denotes	terminus
T6498	12295-12297	IN	denotes	of
T6499	12298-12302	NN	denotes	Acdp
T6500	12302-12303	HYPH	denotes	-
T6501	12303-12304	CD	denotes	1
T6502	12304-12305	.	denotes	.
T6503	12305-12343	sentence	denotes	Lane 1, 10 μg of HEK293 cell lysates.
T6504	12306-12310	NN	denotes	Lane
T6506	12311-12312	CD	denotes	1
T6507	12312-12314	,	denotes	,
T6508	12314-12316	CD	denotes	10
T6505	12317-12319	NNS	denotes	μg
T6509	12320-12322	IN	denotes	of
T6510	12323-12329	NN	denotes	HEK293
T6512	12330-12334	NN	denotes	cell
T6511	12335-12342	NNS	denotes	lysates
T6513	12342-12343	.	denotes	.
T6514	12343-12394	sentence	denotes	Lane 2 and 3, 100 μg of 3T3 and PC12 cell lysates.
T6515	12344-12348	NN	denotes	Lane
T6516	12349-12350	CD	denotes	2
T6518	12351-12354	CC	denotes	and
T6519	12355-12356	CD	denotes	3
T6520	12356-12358	,	denotes	,
T6521	12358-12361	CD	denotes	100
T6517	12362-12364	NNS	denotes	μg
T6522	12365-12367	IN	denotes	of
T6523	12368-12371	NN	denotes	3T3
T6525	12372-12375	CC	denotes	and
T6526	12376-12380	NN	denotes	PC12
T6527	12381-12385	NN	denotes	cell
T6524	12386-12393	NNS	denotes	lysates
T6528	12393-12394	.	denotes	.
T6553	12405-12416	JJ	denotes	Subcellular
T6554	12417-12429	NN	denotes	localization
T6555	12430-12432	IN	denotes	of
T6556	12433-12438	NN	denotes	Acdp1
T6557	12439-12441	IN	denotes	in
T6558	12442-12453	NN	denotes	hippocampus
T6559	12454-12461	NNS	denotes	neurons
T6560	12461-12462	.	denotes	.
T6561	12462-12550	sentence	denotes	A series of confocal images from a cultured neuron stained with an anti-Acdp1 antibody.
T6562	12463-12464	DT	denotes	A
T6563	12465-12471	NN	denotes	series
T6564	12472-12474	IN	denotes	of
T6565	12475-12483	JJ	denotes	confocal
T6566	12484-12490	NNS	denotes	images
T6567	12491-12495	IN	denotes	from
T6568	12496-12497	DT	denotes	a
T6570	12498-12506	VBN	denotes	cultured
T6569	12507-12513	NN	denotes	neuron
T6571	12514-12521	VBN	denotes	stained
T6572	12522-12526	IN	denotes	with
T6573	12527-12529	DT	denotes	an
T6575	12530-12540	JJ	denotes	anti-Acdp1
T6574	12541-12549	NN	denotes	antibody
T6576	12549-12550	.	denotes	.
T6577	12550-12661	sentence	denotes	The step of each imaging section is 0.5 μm, from the surface of the neuron (0 μm) to the middle plan (4.5 μm).
T6578	12551-12554	DT	denotes	The
T6579	12555-12559	NN	denotes	step
T6581	12560-12562	IN	denotes	of
T6582	12563-12567	DT	denotes	each
T6584	12568-12575	NN	denotes	imaging
T6583	12576-12583	NN	denotes	section
T6580	12584-12586	VBZ	denotes	is
T6585	12587-12590	CD	denotes	0.5
T6586	12591-12593	NNS	denotes	μm
T6587	12593-12595	,	denotes	,
T6588	12595-12599	IN	denotes	from
T6589	12600-12603	DT	denotes	the
T6590	12604-12611	NN	denotes	surface
T6591	12612-12614	IN	denotes	of
T6592	12615-12618	DT	denotes	the
T6593	12619-12625	NN	denotes	neuron
T6594	12626-12627	-LRB-	denotes	(
T6596	12627-12628	CD	denotes	0
T6595	12629-12631	NNS	denotes	μm
T6597	12631-12632	-RRB-	denotes	)
T6598	12633-12635	IN	denotes	to
T6599	12636-12639	DT	denotes	the
T6601	12640-12646	JJ	denotes	middle
T6600	12647-12651	NN	denotes	plan
T6602	12652-12653	-LRB-	denotes	(
T6604	12653-12656	CD	denotes	4.5
T6603	12657-12659	NNS	denotes	μm
T6605	12659-12660	-RRB-	denotes	)
T6606	12660-12661	.	denotes	.
T3529	12674-12682	IN	denotes	Although
T3531	12683-12686	DT	denotes	the
T3533	12687-12692	JJ	denotes	exact
T3532	12693-12702	NNS	denotes	functions
T3534	12703-12705	IN	denotes	of
T3535	12706-12709	DT	denotes	the
T3537	12710-12714	NN	denotes	ACDP
T3538	12715-12719	NN	denotes	gene
T3536	12720-12726	NN	denotes	family
T3539	12727-12730	VBP	denotes	are
T3540	12731-12734	RB	denotes	not
T3541	12735-12738	RB	denotes	yet
T3530	12739-12749	VBN	denotes	elucidated
T3543	12749-12751	,	denotes	,
T3544	12751-12758	JJ	denotes	several
T3545	12759-12764	NNS	denotes	lines
T3546	12765-12767	IN	denotes	of
T3547	12768-12776	NN	denotes	evidence
T3542	12777-12784	VBP	denotes	suggest
T3548	12785-12789	IN	denotes	that
T3550	12790-12794	NN	denotes	ACDP
T3551	12795-12800	NNS	denotes	genes
T3552	12801-12804	MD	denotes	may
T3549	12805-12809	VB	denotes	play
T3553	12810-12812	DT	denotes	an
T3555	12813-12822	JJ	denotes	important
T3554	12823-12827	NN	denotes	role
T3556	12828-12830	IN	denotes	in
T3557	12831-12841	JJ	denotes	biological
T3558	12842-12851	NNS	denotes	processes
T3559	12851-12852	.	denotes	.
T3560	12852-13100	sentence	denotes	First, these genes are evolutionarily conserved in many phylogenetically divergent species; Second, multiple genes are present in a species; Third, these genes are ubiquitously expressed throughout development and adult tissues (unpublished data).
T3561	12853-12858	RB	denotes	First
T3563	12858-12860	,	denotes	,
T3564	12860-12865	DT	denotes	these
T3565	12866-12871	NNS	denotes	genes
T3566	12872-12875	VBP	denotes	are
T3567	12876-12890	RB	denotes	evolutionarily
T3562	12891-12900	VBN	denotes	conserved
T3569	12901-12903	IN	denotes	in
T3570	12904-12908	JJ	denotes	many
T3572	12909-12925	RB	denotes	phylogenetically
T3573	12926-12935	JJ	denotes	divergent
T3571	12936-12943	NNS	denotes	species
T3574	12943-12944	:	denotes	;
T3575	12945-12951	RB	denotes	Second
T3577	12951-12953	,	denotes	,
T3578	12953-12961	JJ	denotes	multiple
T3579	12962-12967	NNS	denotes	genes
T3576	12968-12971	VBP	denotes	are
T3580	12972-12979	JJ	denotes	present
T3581	12980-12982	IN	denotes	in
T3582	12983-12984	DT	denotes	a
T3583	12985-12992	NN	denotes	species
T3584	12992-12993	:	denotes	;
T3585	12994-12999	RB	denotes	Third
T3586	12999-13001	,	denotes	,
T3587	13001-13006	DT	denotes	these
T3588	13007-13012	NNS	denotes	genes
T3589	13013-13016	VBP	denotes	are
T3590	13017-13029	RB	denotes	ubiquitously
T3568	13030-13039	VBN	denotes	expressed
T3591	13040-13050	IN	denotes	throughout
T3592	13051-13062	NN	denotes	development
T3594	13063-13066	CC	denotes	and
T3595	13067-13072	JJ	denotes	adult
T3593	13073-13080	NNS	denotes	tissues
T3596	13081-13082	-LRB-	denotes	(
T3598	13082-13093	JJ	denotes	unpublished
T3597	13094-13098	NNS	denotes	data
T3599	13098-13099	-RRB-	denotes	)
T3600	13099-13100	.	denotes	.
T3601	13100-13256	sentence	denotes	The cloning and characterization of the mouse Acdp gene family are a very important step towards the elucidation of the functions of this multigene family.
T3602	13101-13104	DT	denotes	The
T3603	13105-13112	NN	denotes	cloning
T3605	13113-13116	CC	denotes	and
T3606	13117-13133	NN	denotes	characterization
T3607	13134-13136	IN	denotes	of
T3608	13137-13140	DT	denotes	the
T3610	13141-13146	NN	denotes	mouse
T3611	13147-13151	NN	denotes	Acdp
T3612	13152-13156	NN	denotes	gene
T3609	13157-13163	NN	denotes	family
T3604	13164-13167	VBP	denotes	are
T3613	13168-13169	DT	denotes	a
T3615	13170-13174	RB	denotes	very
T3616	13175-13184	JJ	denotes	important
T3614	13185-13189	NN	denotes	step
T3617	13190-13197	IN	denotes	towards
T3618	13198-13201	DT	denotes	the
T3619	13202-13213	NN	denotes	elucidation
T3620	13214-13216	IN	denotes	of
T3621	13217-13220	DT	denotes	the
T3622	13221-13230	NNS	denotes	functions
T3623	13231-13233	IN	denotes	of
T3624	13234-13238	DT	denotes	this
T3626	13239-13248	JJ	denotes	multigene
T3625	13249-13255	NN	denotes	family
T3627	13255-13256	.	denotes	.
T3628	13256-13398	sentence	denotes	Sequence homology analyses revealed that Acdp proteins shared very strong AA homologies to the bacteria CorC protein and yeast Amip3 protein.
T3629	13257-13265	NN	denotes	Sequence
T3631	13266-13274	NN	denotes	homology
T3630	13275-13283	NNS	denotes	analyses
T3632	13284-13292	VBD	denotes	revealed
T3633	13293-13297	IN	denotes	that
T3635	13298-13302	NN	denotes	Acdp
T3636	13303-13311	NN	denotes	proteins
T3634	13312-13318	VBD	denotes	shared
T3637	13319-13323	RB	denotes	very
T3638	13324-13330	JJ	denotes	strong
T3640	13331-13333	NN	denotes	AA
T3639	13334-13344	NNS	denotes	homologies
T3641	13345-13347	IN	denotes	to
T3642	13348-13351	DT	denotes	the
T3644	13352-13360	NNS	denotes	bacteria
T3645	13361-13365	NN	denotes	CorC
T3643	13366-13373	NN	denotes	protein
T3646	13374-13377	CC	denotes	and
T3647	13378-13383	NN	denotes	yeast
T3649	13384-13389	NN	denotes	Amip3
T3648	13390-13397	NN	denotes	protein
T3650	13397-13398	.	denotes	.
T3651	13398-13502	sentence	denotes	CorA, B, C and D belong to a protein family involved in both influx and efflux of magnesium and cobalt.
T3652	13399-13403	NN	denotes	CorA
T3654	13403-13405	,	denotes	,
T3655	13405-13406	NN	denotes	B
T3656	13406-13408	,	denotes	,
T3657	13408-13409	NN	denotes	C
T3658	13410-13413	CC	denotes	and
T3659	13414-13415	NN	denotes	D
T3653	13416-13422	VBP	denotes	belong
T3660	13423-13425	IN	denotes	to
T3661	13426-13427	DT	denotes	a
T3663	13428-13435	NN	denotes	protein
T3662	13436-13442	NN	denotes	family
T3664	13443-13451	VBN	denotes	involved
T3665	13452-13454	IN	denotes	in
T3666	13455-13459	CC	denotes	both
T3667	13460-13466	NN	denotes	influx
T3668	13467-13470	CC	denotes	and
T3669	13471-13477	NN	denotes	efflux
T3670	13478-13480	IN	denotes	of
T3671	13481-13490	NN	denotes	magnesium
T3672	13491-13494	CC	denotes	and
T3673	13495-13501	NN	denotes	cobalt
T3674	13501-13502	.	denotes	.
T3675	13502-13580	sentence	denotes	It has been shown that CorA mutants confer resistance to cobalt toxicity [9].
T3676	13503-13505	PRP	denotes	It
T3678	13506-13509	VBZ	denotes	has
T3679	13510-13514	VBN	denotes	been
T3677	13515-13520	VBN	denotes	shown
T3680	13521-13525	IN	denotes	that
T3682	13526-13530	NN	denotes	CorA
T3683	13531-13538	NNS	denotes	mutants
T3681	13539-13545	VBP	denotes	confer
T3684	13546-13556	NN	denotes	resistance
T3685	13557-13559	IN	denotes	to
T3686	13560-13566	NN	denotes	cobalt
T3687	13567-13575	NN	denotes	toxicity
T3688	13576-13577	-LRB-	denotes	[
T3689	13577-13578	CD	denotes	9
T3690	13578-13579	-RRB-	denotes	]
T3691	13579-13580	.	denotes	.
T3692	13580-13694	sentence	denotes	Amip3 appears to be a homologous to CorC protein which is involved in efflux of magnesium and cobalt in bacteria.
T3693	13581-13586	NN	denotes	Amip3
T3694	13587-13594	VBZ	denotes	appears
T3695	13595-13597	TO	denotes	to
T3696	13598-13600	VB	denotes	be
T3697	13601-13602	DT	denotes	a
T3698	13603-13613	JJ	denotes	homologous
T3699	13614-13616	IN	denotes	to
T3700	13617-13621	NN	denotes	CorC
T3701	13622-13629	NN	denotes	protein
T3702	13630-13635	WDT	denotes	which
T3704	13636-13638	VBZ	denotes	is
T3703	13639-13647	VBN	denotes	involved
T3705	13648-13650	IN	denotes	in
T3706	13651-13657	NN	denotes	efflux
T3707	13658-13660	IN	denotes	of
T3708	13661-13670	NN	denotes	magnesium
T3709	13671-13674	CC	denotes	and
T3710	13675-13681	NN	denotes	cobalt
T3711	13682-13684	IN	denotes	in
T3712	13685-13693	NNS	denotes	bacteria
T3713	13693-13694	.	denotes	.
T3714	13694-13745	sentence	denotes	Amip3 mutants confer resistant to copper toxicity.
T3715	13695-13700	NN	denotes	Amip3
T3716	13701-13708	NNS	denotes	mutants
T3717	13709-13715	VBP	denotes	confer
T3718	13716-13725	NN	denotes	resistant
T3719	13726-13728	IN	denotes	to
T3720	13729-13735	NN	denotes	copper
T3721	13736-13744	NN	denotes	toxicity
T3722	13744-13745	.	denotes	.
T3723	13745-13907	sentence	denotes	Acdp proteins also possess the domains that are found in bacteria CorC and yeast Amip3 proteins, such as the CBS domains, DUF21 domain and transmembrane domains.
T3724	13746-13750	NN	denotes	Acdp
T3726	13751-13759	NN	denotes	proteins
T3727	13760-13764	RB	denotes	also
T3725	13765-13772	VBP	denotes	possess
T3728	13773-13776	DT	denotes	the
T3729	13777-13784	NNS	denotes	domains
T3730	13785-13789	WDT	denotes	that
T3732	13790-13793	VBP	denotes	are
T3731	13794-13799	VBN	denotes	found
T3733	13800-13802	IN	denotes	in
T3734	13803-13811	NNS	denotes	bacteria
T3735	13812-13816	NN	denotes	CorC
T3737	13817-13820	CC	denotes	and
T3738	13821-13826	NN	denotes	yeast
T3739	13827-13832	NN	denotes	Amip3
T3736	13833-13841	NN	denotes	proteins
T3740	13841-13843	,	denotes	,
T3741	13843-13847	JJ	denotes	such
T3742	13848-13850	IN	denotes	as
T3743	13851-13854	DT	denotes	the
T3745	13855-13858	NN	denotes	CBS
T3744	13859-13866	NNS	denotes	domains
T3746	13866-13868	,	denotes	,
T3747	13868-13873	NN	denotes	DUF21
T3748	13874-13880	NN	denotes	domain
T3749	13881-13884	CC	denotes	and
T3750	13885-13898	NN	denotes	transmembrane
T3751	13899-13906	NNS	denotes	domains
T3752	13906-13907	.	denotes	.
T3753	13907-14051	sentence	denotes	In addition, a cNMP-binding domain was found in all Acdp proteins, which is usually present in ion channels and cNMP-dependent kinases [10-13].
T3754	13908-13910	IN	denotes	In
T3756	13911-13919	NN	denotes	addition
T3757	13919-13921	,	denotes	,
T3758	13921-13922	DT	denotes	a
T3760	13923-13927	NN	denotes	cNMP
T3762	13927-13928	HYPH	denotes	-
T3761	13928-13935	VBG	denotes	binding
T3759	13936-13942	NN	denotes	domain
T3763	13943-13946	VBD	denotes	was
T3755	13947-13952	VBN	denotes	found
T3764	13953-13955	IN	denotes	in
T3765	13956-13959	DT	denotes	all
T3767	13960-13964	NN	denotes	Acdp
T3766	13965-13973	NN	denotes	proteins
T3768	13973-13975	,	denotes	,
T3769	13975-13980	WDT	denotes	which
T3770	13981-13983	VBZ	denotes	is
T3771	13984-13991	RB	denotes	usually
T3772	13992-13999	JJ	denotes	present
T3773	14000-14002	IN	denotes	in
T3774	14003-14006	NN	denotes	ion
T3775	14007-14015	NNS	denotes	channels
T3776	14016-14019	CC	denotes	and
T3777	14020-14024	NN	denotes	cNMP
T3779	14024-14025	HYPH	denotes	-
T3780	14025-14034	JJ	denotes	dependent
T3778	14035-14042	NNS	denotes	kinases
T3781	14043-14044	-LRB-	denotes	[
T3782	14044-14046	CD	denotes	10
T3783	14046-14047	SYM	denotes	-
T3784	14047-14049	CD	denotes	13
T3785	14049-14050	-RRB-	denotes	]
T3786	14050-14051	.	denotes	.
T3787	14051-14203	sentence	denotes	Using antibody produced by Acdp1 C-terminal peptide, we have shown that Acdp1 is predominantly localized on the plasma membrane in hippocampus neurons.
T3788	14052-14057	VBG	denotes	Using
T3790	14058-14066	NN	denotes	antibody
T3791	14067-14075	VBN	denotes	produced
T3792	14076-14078	IN	denotes	by
T3793	14079-14084	NN	denotes	Acdp1
T3795	14085-14086	NN	denotes	C
T3797	14086-14087	HYPH	denotes	-
T3796	14087-14095	JJ	denotes	terminal
T3794	14096-14103	NN	denotes	peptide
T3798	14103-14105	,	denotes	,
T3799	14105-14107	PRP	denotes	we
T3800	14108-14112	VBP	denotes	have
T3789	14113-14118	VBN	denotes	shown
T3801	14119-14123	IN	denotes	that
T3803	14124-14129	NN	denotes	Acdp1
T3804	14130-14132	VBZ	denotes	is
T3805	14133-14146	RB	denotes	predominantly
T3802	14147-14156	VBN	denotes	localized
T3806	14157-14159	IN	denotes	on
T3807	14160-14163	DT	denotes	the
T3809	14164-14170	NN	denotes	plasma
T3808	14171-14179	NN	denotes	membrane
T3810	14180-14182	IN	denotes	in
T3811	14183-14194	NN	denotes	hippocampus
T3812	14195-14202	NNS	denotes	neurons
T3813	14202-14203	.	denotes	.
T3814	14203-14317	sentence	denotes	In our previous study, we found human ACDP proteins are predominantly localized in the nucleus in HeLa cells [1].
T3815	14204-14206	IN	denotes	In
T3817	14207-14210	PRP$	denotes	our
T3819	14211-14219	JJ	denotes	previous
T3818	14220-14225	NN	denotes	study
T3820	14225-14227	,	denotes	,
T3821	14227-14229	PRP	denotes	we
T3816	14230-14235	VBD	denotes	found
T3822	14236-14241	JJ	denotes	human
T3824	14242-14246	NN	denotes	ACDP
T3823	14247-14255	NN	denotes	proteins
T3826	14256-14259	VBP	denotes	are
T3827	14260-14273	RB	denotes	predominantly
T3825	14274-14283	VBN	denotes	localized
T3828	14284-14286	IN	denotes	in
T3829	14287-14290	DT	denotes	the
T3830	14291-14298	NN	denotes	nucleus
T3831	14299-14301	IN	denotes	in
T3832	14302-14306	NN	denotes	HeLa
T3833	14307-14312	NNS	denotes	cells
T3834	14313-14314	-LRB-	denotes	[
T3835	14314-14315	CD	denotes	1
T3836	14315-14316	-RRB-	denotes	]
T3837	14316-14317	.	denotes	.
T3838	14317-14505	sentence	denotes	The discrepancy for Acdp localization in neuronal cells could be caused by non-specificity for previous antibody which was produced by the recombinant ACD domain, or different cells used.
T3839	14318-14321	DT	denotes	The
T3840	14322-14333	NN	denotes	discrepancy
T3842	14334-14337	IN	denotes	for
T3843	14338-14342	NN	denotes	Acdp
T3844	14343-14355	NN	denotes	localization
T3845	14356-14358	IN	denotes	in
T3846	14359-14367	JJ	denotes	neuronal
T3847	14368-14373	NNS	denotes	cells
T3848	14374-14379	MD	denotes	could
T3849	14380-14382	VB	denotes	be
T3841	14383-14389	VBN	denotes	caused
T3850	14390-14392	IN	denotes	by
T3851	14393-14408	NN	denotes	non-specificity
T3852	14409-14412	IN	denotes	for
T3853	14413-14421	JJ	denotes	previous
T3854	14422-14430	NN	denotes	antibody
T3855	14431-14436	WDT	denotes	which
T3857	14437-14440	VBD	denotes	was
T3856	14441-14449	VBN	denotes	produced
T3858	14450-14452	IN	denotes	by
T3859	14453-14456	DT	denotes	the
T3861	14457-14468	JJ	denotes	recombinant
T3862	14469-14472	NN	denotes	ACD
T3860	14473-14479	NN	denotes	domain
T3863	14479-14481	,	denotes	,
T3864	14481-14483	CC	denotes	or
T3865	14484-14493	JJ	denotes	different
T3866	14494-14499	NNS	denotes	cells
T3867	14500-14504	VBN	denotes	used
T3868	14504-14505	.	denotes	.
T3869	14505-14693	sentence	denotes	In current study, the Acdp1 C-terminus antibody only recognizes Acdp1 in brain tissue extracts as well as in HEK293, 3T3 and PC12 cell lysates, suggesting the specificity of the antibody.
T3870	14506-14508	IN	denotes	In
T3872	14509-14516	JJ	denotes	current
T3873	14517-14522	NN	denotes	study
T3874	14522-14524	,	denotes	,
T3875	14524-14527	DT	denotes	the
T3877	14528-14533	NN	denotes	Acdp1
T3878	14534-14535	NN	denotes	C
T3880	14535-14536	HYPH	denotes	-
T3879	14536-14544	NN	denotes	terminus
T3876	14545-14553	NN	denotes	antibody
T3881	14554-14558	RB	denotes	only
T3871	14559-14569	VBZ	denotes	recognizes
T3882	14570-14575	NN	denotes	Acdp1
T3883	14576-14578	IN	denotes	in
T3884	14579-14584	NN	denotes	brain
T3885	14585-14591	NN	denotes	tissue
T3886	14592-14600	NNS	denotes	extracts
T3887	14601-14603	RB	denotes	as
T3889	14604-14608	RB	denotes	well
T3888	14609-14611	IN	denotes	as
T3890	14612-14614	IN	denotes	in
T3891	14615-14621	NN	denotes	HEK293
T3893	14621-14623	,	denotes	,
T3894	14623-14626	NN	denotes	3T3
T3895	14627-14630	CC	denotes	and
T3896	14631-14635	NN	denotes	PC12
T3897	14636-14640	NN	denotes	cell
T3892	14641-14648	NNS	denotes	lysates
T3898	14648-14650	,	denotes	,
T3899	14650-14660	VBG	denotes	suggesting
T3900	14661-14664	DT	denotes	the
T3901	14665-14676	NN	denotes	specificity
T3902	14677-14679	IN	denotes	of
T3903	14680-14683	DT	denotes	the
T3904	14684-14692	NN	denotes	antibody
T3905	14692-14693	.	denotes	.
T3906	14693-14785	sentence	denotes	Our observations suggested that Acdp might be involved in ion transport in mammalian cells.
T3907	14694-14697	PRP$	denotes	Our
T3908	14698-14710	NNS	denotes	observations
T3909	14711-14720	VBD	denotes	suggested
T3910	14721-14725	IN	denotes	that
T3912	14726-14730	NN	denotes	Acdp
T3913	14731-14736	MD	denotes	might
T3914	14737-14739	VB	denotes	be
T3911	14740-14748	VBN	denotes	involved
T3915	14749-14751	IN	denotes	in
T3916	14752-14755	NN	denotes	ion
T3917	14756-14765	NN	denotes	transport
T3918	14766-14768	IN	denotes	in
T3919	14769-14778	JJ	denotes	mammalian
T3920	14779-14784	NNS	denotes	cells
T3921	14784-14785	.	denotes	.
T3922	14785-14891	sentence	denotes	However, more detailed functional studies are needed to demonstrate the real functions of these proteins.
T3923	14786-14793	RB	denotes	However
T3925	14793-14795	,	denotes	,
T3926	14795-14799	RBR	denotes	more
T3927	14800-14808	JJ	denotes	detailed
T3929	14809-14819	JJ	denotes	functional
T3928	14820-14827	NNS	denotes	studies
T3930	14828-14831	VBP	denotes	are
T3924	14832-14838	VBN	denotes	needed
T3931	14839-14841	TO	denotes	to
T3932	14842-14853	VB	denotes	demonstrate
T3933	14854-14857	DT	denotes	the
T3935	14858-14862	JJ	denotes	real
T3934	14863-14872	NNS	denotes	functions
T3936	14873-14875	IN	denotes	of
T3937	14876-14881	DT	denotes	these
T3938	14882-14890	NN	denotes	proteins
T3939	14890-14891	.	denotes	.
T4051	14905-14908	PRP$	denotes	Our
T4053	14909-14917	JJ	denotes	previous
T4052	14918-14922	NN	denotes	work
T4055	14923-14926	VBZ	denotes	has
T4054	14927-14936	VBN	denotes	described
T4056	14937-14942	JJ	denotes	human
T4058	14943-14947	NN	denotes	ACDP
T4057	14948-14953	NNS	denotes	genes
T4059	14954-14955	-LRB-	denotes	[
T4060	14955-14956	CD	denotes	1
T4061	14956-14957	-RRB-	denotes	]
T4062	14957-14958	.	denotes	.
T4063	14958-15226	sentence	denotes	The new information of the current work includes: 1). It is the first time to report the existence of multiple Acdp genes in other mammals in addition to human, while Acdp appears to be a single copy gene in lower organisms such as in C. elegans, yeasts and bacteria.
T4064	14959-14962	DT	denotes	The
T4066	14963-14966	JJ	denotes	new
T4065	14967-14978	NN	denotes	information
T4068	14979-14981	IN	denotes	of
T4069	14982-14985	DT	denotes	the
T4071	14986-14993	JJ	denotes	current
T4070	14994-14998	NN	denotes	work
T4067	14999-15007	VBZ	denotes	includes
T4073	15007-15009	:	denotes	:
T4074	15009-15010	LS	denotes	1
T4075	15010-15011	-RRB-	denotes	)
T4076	15011-15012	,	denotes	.
T4077	15013-15015	PRP	denotes	It
T4072	15016-15018	VBZ	denotes	is
T4078	15019-15022	DT	denotes	the
T4080	15023-15028	JJ	denotes	first
T4079	15029-15033	NN	denotes	time
T4081	15034-15036	TO	denotes	to
T4082	15037-15043	VB	denotes	report
T4083	15044-15047	DT	denotes	the
T4084	15048-15057	NN	denotes	existence
T4085	15058-15060	IN	denotes	of
T4086	15061-15069	JJ	denotes	multiple
T4088	15070-15074	NN	denotes	Acdp
T4087	15075-15080	NNS	denotes	genes
T4089	15081-15083	IN	denotes	in
T4090	15084-15089	JJ	denotes	other
T4091	15090-15097	NNS	denotes	mammals
T4092	15098-15100	IN	denotes	in
T4093	15101-15109	NN	denotes	addition
T4094	15110-15112	IN	denotes	to
T4095	15113-15118	NN	denotes	human
T4096	15118-15120	,	denotes	,
T4097	15120-15125	IN	denotes	while
T4099	15126-15130	NN	denotes	Acdp
T4098	15131-15138	VBZ	denotes	appears
T4100	15139-15141	TO	denotes	to
T4101	15142-15144	VB	denotes	be
T4102	15145-15146	DT	denotes	a
T4104	15147-15153	JJ	denotes	single
T4105	15154-15158	NN	denotes	copy
T4103	15159-15163	NN	denotes	gene
T4106	15164-15166	IN	denotes	in
T4107	15167-15172	JJR	denotes	lower
T4108	15173-15182	NNS	denotes	organisms
T4109	15183-15187	JJ	denotes	such
T4110	15188-15190	IN	denotes	as
T4111	15191-15193	IN	denotes	in
T4112	15194-15196	NNP	denotes	C.
T4113	15197-15204	NNP	denotes	elegans
T4114	15204-15206	,	denotes	,
T4115	15206-15212	NNS	denotes	yeasts
T4116	15213-15216	CC	denotes	and
T4117	15217-15225	NNS	denotes	bacteria
T4118	15225-15226	.	denotes	.
T4119	15226-15854	sentence	denotes	We have also suggested the evolutionary relationships of Acdp genes in different species (phylogenetic tree); 2). Molecular cloning and characterization of murine Acdp gene family are essential for study of this novel gene family in animal model, e.g. for generation of knockout or transgenic models; 3). We have demonstrated both DNA and amino acid conservations between mouse and human for each Acdp gene and the whole Acdp gene family, which provide important information for the possibility of functional redundancy or overlap between Acdp members; 4). We have generated antibodies specific for Acdp1 and all Acdp proteins.
T4120	15227-15229	PRP	denotes	We
T4122	15230-15234	VBP	denotes	have
T4123	15235-15239	RB	denotes	also
T4121	15240-15249	VBN	denotes	suggested
T4125	15250-15253	DT	denotes	the
T4127	15254-15266	JJ	denotes	evolutionary
T4126	15267-15280	NNS	denotes	relationships
T4128	15281-15283	IN	denotes	of
T4129	15284-15288	NN	denotes	Acdp
T4130	15289-15294	NNS	denotes	genes
T4131	15295-15297	IN	denotes	in
T4132	15298-15307	JJ	denotes	different
T4133	15308-15315	NNS	denotes	species
T4134	15316-15317	-LRB-	denotes	(
T4136	15317-15329	JJ	denotes	phylogenetic
T4135	15330-15334	NN	denotes	tree
T4137	15334-15335	-RRB-	denotes	)
T4138	15335-15336	:	denotes	;
T4139	15337-15338	LS	denotes	2
T4141	15338-15339	-RRB-	denotes	)
T4142	15339-15340	,	denotes	.
T4143	15341-15350	JJ	denotes	Molecular
T4144	15351-15358	NN	denotes	cloning
T4145	15359-15362	CC	denotes	and
T4146	15363-15379	NN	denotes	characterization
T4147	15380-15382	IN	denotes	of
T4148	15383-15389	JJ	denotes	murine
T4150	15390-15394	NN	denotes	Acdp
T4151	15395-15399	NN	denotes	gene
T4149	15400-15406	NN	denotes	family
T4140	15407-15410	VBP	denotes	are
T4152	15411-15420	JJ	denotes	essential
T4153	15421-15424	IN	denotes	for
T4154	15425-15430	NN	denotes	study
T4155	15431-15433	IN	denotes	of
T4156	15434-15438	DT	denotes	this
T4158	15439-15444	JJ	denotes	novel
T4159	15445-15449	NN	denotes	gene
T4157	15450-15456	NN	denotes	family
T4160	15457-15459	IN	denotes	in
T4161	15460-15466	NN	denotes	animal
T4162	15467-15472	NN	denotes	model
T4163	15472-15474	,	denotes	,
T4164	15474-15478	FW	denotes	e.g.
T4165	15479-15482	IN	denotes	for
T4166	15483-15493	NN	denotes	generation
T4167	15494-15496	IN	denotes	of
T4168	15497-15505	NN	denotes	knockout
T4170	15506-15508	CC	denotes	or
T4171	15509-15519	JJ	denotes	transgenic
T4169	15520-15526	NNS	denotes	models
T4172	15526-15527	:	denotes	;
T4173	15528-15529	LS	denotes	3
T4175	15529-15530	-RRB-	denotes	)
T4176	15530-15531	,	denotes	.
T4177	15532-15534	PRP	denotes	We
T4178	15535-15539	VBP	denotes	have
T4174	15540-15552	VBN	denotes	demonstrated
T4179	15553-15557	CC	denotes	both
T4180	15558-15561	NN	denotes	DNA
T4182	15562-15565	CC	denotes	and
T4183	15566-15571	NN	denotes	amino
T4184	15572-15576	NN	denotes	acid
T4181	15577-15590	NNS	denotes	conservations
T4185	15591-15598	IN	denotes	between
T4186	15599-15604	NN	denotes	mouse
T4187	15605-15608	CC	denotes	and
T4188	15609-15614	NN	denotes	human
T4189	15615-15618	IN	denotes	for
T4190	15619-15623	DT	denotes	each
T4192	15624-15628	NN	denotes	Acdp
T4191	15629-15633	NN	denotes	gene
T4193	15634-15637	CC	denotes	and
T4194	15638-15641	DT	denotes	the
T4196	15642-15647	JJ	denotes	whole
T4197	15648-15652	NN	denotes	Acdp
T4198	15653-15657	NN	denotes	gene
T4195	15658-15664	NN	denotes	family
T4199	15664-15666	,	denotes	,
T4200	15666-15671	WDT	denotes	which
T4201	15672-15679	VBP	denotes	provide
T4202	15680-15689	JJ	denotes	important
T4203	15690-15701	NN	denotes	information
T4204	15702-15705	IN	denotes	for
T4205	15706-15709	DT	denotes	the
T4206	15710-15721	NN	denotes	possibility
T4207	15722-15724	IN	denotes	of
T4208	15725-15735	JJ	denotes	functional
T4209	15736-15746	NN	denotes	redundancy
T4210	15747-15749	CC	denotes	or
T4211	15750-15757	NN	denotes	overlap
T4212	15758-15765	IN	denotes	between
T4213	15766-15770	NN	denotes	Acdp
T4214	15771-15778	NNS	denotes	members
T4215	15778-15779	:	denotes	;
T4216	15780-15781	LS	denotes	4
T4217	15781-15782	-RRB-	denotes	)
T4218	15782-15783	,	denotes	.
T4219	15784-15786	PRP	denotes	We
T4220	15787-15791	VBP	denotes	have
T4124	15792-15801	VBN	denotes	generated
T4221	15802-15812	NNS	denotes	antibodies
T4222	15813-15821	JJ	denotes	specific
T4223	15822-15825	IN	denotes	for
T4224	15826-15831	NN	denotes	Acdp1
T4225	15832-15835	CC	denotes	and
T4226	15836-15839	DT	denotes	all
T4228	15840-15844	NN	denotes	Acdp
T4227	15845-15853	NN	denotes	proteins
T4229	15853-15854	.	denotes	.
T4230	15854-15925	sentence	denotes	The Acdp1 C-terminus antibody appears to specifically recognize Acdp1.
T4231	15855-15858	DT	denotes	The
T4233	15859-15864	NN	denotes	Acdp1
T4234	15865-15866	NN	denotes	C
T4236	15866-15867	HYPH	denotes	-
T4235	15867-15875	NN	denotes	terminus
T4232	15876-15884	NN	denotes	antibody
T4237	15885-15892	VBZ	denotes	appears
T4238	15893-15895	TO	denotes	to
T4240	15896-15908	RB	denotes	specifically
T4239	15909-15918	VB	denotes	recognize
T4241	15919-15924	NN	denotes	Acdp1
T4242	15924-15925	.	denotes	.
T4243	15925-16052	sentence	denotes	Using this antibody, we have demonstrated that Acdp1 is predominantly localized on the plasma membrane in hippocampus neurons.
T4244	15926-15931	VBG	denotes	Using
T4246	15932-15936	DT	denotes	this
T4247	15937-15945	NN	denotes	antibody
T4248	15945-15947	,	denotes	,
T4249	15947-15949	PRP	denotes	we
T4250	15950-15954	VBP	denotes	have
T4245	15955-15967	VBN	denotes	demonstrated
T4251	15968-15972	IN	denotes	that
T4253	15973-15978	NN	denotes	Acdp1
T4254	15979-15981	VBZ	denotes	is
T4255	15982-15995	RB	denotes	predominantly
T4252	15996-16005	VBN	denotes	localized
T4256	16006-16008	IN	denotes	on
T4257	16009-16012	DT	denotes	the
T4259	16013-16019	NN	denotes	plasma
T4258	16020-16028	NN	denotes	membrane
T4260	16029-16031	IN	denotes	in
T4261	16032-16043	NN	denotes	hippocampus
T4262	16044-16051	NNS	denotes	neurons
T4263	16051-16052	.	denotes	.
T4264	16052-16162	sentence	denotes	These results represent an important step towards the characterization of functions for the Acdp gene family.
T4265	16053-16058	DT	denotes	These
T4266	16059-16066	NNS	denotes	results
T4267	16067-16076	VBP	denotes	represent
T4268	16077-16079	DT	denotes	an
T4270	16080-16089	JJ	denotes	important
T4269	16090-16094	NN	denotes	step
T4271	16095-16102	IN	denotes	towards
T4272	16103-16106	DT	denotes	the
T4273	16107-16123	NN	denotes	characterization
T4274	16124-16126	IN	denotes	of
T4275	16127-16136	NNS	denotes	functions
T4276	16137-16140	IN	denotes	for
T4277	16141-16144	DT	denotes	the
T4279	16145-16149	NN	denotes	Acdp
T4280	16150-16154	NN	denotes	gene
T4278	16155-16161	NN	denotes	family
T4281	16161-16162	.	denotes	.
T4392	16173-16177	NN	denotes	cDNA
T4393	16178-16185	NN	denotes	cloning
T4394	16185-16299	sentence	denotes	cDNA cloning of the Acdp gene family was performed based on human homologue sequences as previously reported [2].
T4395	16186-16190	NN	denotes	cDNA
T4396	16191-16198	NN	denotes	cloning
T4398	16199-16201	IN	denotes	of
T4399	16202-16205	DT	denotes	the
T4401	16206-16210	NN	denotes	Acdp
T4402	16211-16215	NN	denotes	gene
T4400	16216-16222	NN	denotes	family
T4403	16223-16226	VBD	denotes	was
T4397	16227-16236	VBN	denotes	performed
T4404	16237-16242	VBN	denotes	based
T4405	16243-16245	IN	denotes	on
T4406	16246-16251	JJ	denotes	human
T4408	16252-16261	NN	denotes	homologue
T4407	16262-16271	NNS	denotes	sequences
T4409	16272-16274	IN	denotes	as
T4411	16275-16285	RB	denotes	previously
T4410	16286-16294	VBN	denotes	reported
T4412	16295-16296	-LRB-	denotes	[
T4413	16296-16297	CD	denotes	2
T4414	16297-16298	-RRB-	denotes	]
T4415	16298-16299	.	denotes	.
T4416	16299-16450	sentence	denotes	Briefly, the human ACDP cDNAs and predicted AA sequences were used to search the mouse EST database for EST markers corresponding to each Acdp member.
T4417	16300-16307	RB	denotes	Briefly
T4419	16307-16309	,	denotes	,
T4420	16309-16312	DT	denotes	the
T4422	16313-16318	JJ	denotes	human
T4423	16319-16323	NN	denotes	ACDP
T4421	16324-16329	NNS	denotes	cDNAs
T4424	16330-16333	CC	denotes	and
T4425	16334-16343	VBN	denotes	predicted
T4427	16344-16346	NN	denotes	AA
T4426	16347-16356	NNS	denotes	sequences
T4428	16357-16361	VBD	denotes	were
T4418	16362-16366	VBN	denotes	used
T4429	16367-16369	TO	denotes	to
T4430	16370-16376	VB	denotes	search
T4431	16377-16380	DT	denotes	the
T4433	16381-16386	NN	denotes	mouse
T4434	16387-16390	NN	denotes	EST
T4432	16391-16399	NN	denotes	database
T4435	16400-16403	IN	denotes	for
T4436	16404-16407	NN	denotes	EST
T4437	16408-16415	NNS	denotes	markers
T4438	16416-16429	VBG	denotes	corresponding
T4439	16430-16432	IN	denotes	to
T4440	16433-16437	DT	denotes	each
T4442	16438-16442	NN	denotes	Acdp
T4441	16443-16449	NN	denotes	member
T4443	16449-16450	.	denotes	.
T4444	16450-16689	sentence	denotes	A forward primer within the human ACDP 5' cDNA coding region (after start codon) and a mouse reverse primer from the mouse EST marker were used to amplify the homologue sequence from mouse cDNA at very low annealing temperature (45–50°C).
T4445	16451-16452	DT	denotes	A
T4447	16453-16460	JJ	denotes	forward
T4446	16461-16467	NN	denotes	primer
T4449	16468-16474	IN	denotes	within
T4450	16475-16478	DT	denotes	the
T4452	16479-16484	JJ	denotes	human
T4453	16485-16489	NN	denotes	ACDP
T4454	16490-16491	CD	denotes	5
T4455	16491-16492	SYM	denotes	'
T4456	16493-16497	NN	denotes	cDNA
T4457	16498-16504	NN	denotes	coding
T4451	16505-16511	NN	denotes	region
T4458	16512-16513	-LRB-	denotes	(
T4459	16513-16518	IN	denotes	after
T4460	16519-16524	NN	denotes	start
T4461	16525-16530	NN	denotes	codon
T4462	16530-16531	-RRB-	denotes	)
T4463	16532-16535	CC	denotes	and
T4464	16536-16537	DT	denotes	a
T4466	16538-16543	NN	denotes	mouse
T4467	16544-16551	JJ	denotes	reverse
T4465	16552-16558	NN	denotes	primer
T4468	16559-16563	IN	denotes	from
T4469	16564-16567	DT	denotes	the
T4471	16568-16573	NN	denotes	mouse
T4472	16574-16577	NN	denotes	EST
T4470	16578-16584	NN	denotes	marker
T4473	16585-16589	VBD	denotes	were
T4448	16590-16594	VBN	denotes	used
T4474	16595-16597	TO	denotes	to
T4475	16598-16605	VB	denotes	amplify
T4476	16606-16609	DT	denotes	the
T4478	16610-16619	NN	denotes	homologue
T4477	16620-16628	NN	denotes	sequence
T4479	16629-16633	IN	denotes	from
T4480	16634-16639	NN	denotes	mouse
T4481	16640-16644	NN	denotes	cDNA
T4482	16645-16647	IN	denotes	at
T4483	16648-16652	RB	denotes	very
T4484	16653-16656	JJ	denotes	low
T4486	16657-16666	VBG	denotes	annealing
T4485	16667-16678	NN	denotes	temperature
T4487	16679-16680	-LRB-	denotes	(
T4488	16680-16682	CD	denotes	45
T4490	16682-16683	SYM	denotes	–
T4489	16683-16685	CD	denotes	50
T4491	16685-16687	NN	denotes	°C
T4492	16687-16688	-RRB-	denotes	)
T4493	16688-16689	.	denotes	.
T4494	16689-16923	sentence	denotes	A nested PCR using an inside reverse primer from the mouse EST sequence and the same human forward primer was then carried out to amplify the specific mouse gene from the first round PCR products at high annealing temperature (62°C).
T4495	16690-16691	DT	denotes	A
T4497	16692-16698	JJ	denotes	nested
T4496	16699-16702	NN	denotes	PCR
T4499	16703-16708	VBG	denotes	using
T4500	16709-16711	DT	denotes	an
T4502	16712-16718	JJ	denotes	inside
T4503	16719-16726	JJ	denotes	reverse
T4501	16727-16733	NN	denotes	primer
T4504	16734-16738	IN	denotes	from
T4505	16739-16742	DT	denotes	the
T4507	16743-16748	NN	denotes	mouse
T4508	16749-16752	NN	denotes	EST
T4506	16753-16761	NN	denotes	sequence
T4509	16762-16765	CC	denotes	and
T4510	16766-16769	DT	denotes	the
T4512	16770-16774	JJ	denotes	same
T4513	16775-16780	JJ	denotes	human
T4514	16781-16788	JJ	denotes	forward
T4511	16789-16795	NN	denotes	primer
T4515	16796-16799	VBD	denotes	was
T4516	16800-16804	RB	denotes	then
T4498	16805-16812	VBN	denotes	carried
T4517	16813-16816	RP	denotes	out
T4518	16817-16819	TO	denotes	to
T4519	16820-16827	VB	denotes	amplify
T4520	16828-16831	DT	denotes	the
T4522	16832-16840	JJ	denotes	specific
T4523	16841-16846	NN	denotes	mouse
T4521	16847-16851	NN	denotes	gene
T4524	16852-16856	IN	denotes	from
T4525	16857-16860	DT	denotes	the
T4527	16861-16866	JJ	denotes	first
T4528	16867-16872	NN	denotes	round
T4529	16873-16876	NN	denotes	PCR
T4526	16877-16885	NNS	denotes	products
T4530	16886-16888	IN	denotes	at
T4531	16889-16893	JJ	denotes	high
T4533	16894-16903	VBG	denotes	annealing
T4532	16904-16915	NN	denotes	temperature
T4534	16916-16917	-LRB-	denotes	(
T4535	16917-16919	CD	denotes	62
T4536	16919-16921	NN	denotes	°C
T4537	16921-16922	-RRB-	denotes	)
T4538	16922-16923	.	denotes	.
T4539	16923-17036	sentence	denotes	The expected PCR products were directly excised from agarose gel and sequenced by an ABI377 automatic sequencer.
T4540	16924-16927	DT	denotes	The
T4542	16928-16936	JJ	denotes	expected
T4543	16937-16940	NN	denotes	PCR
T4541	16941-16949	NNS	denotes	products
T4545	16950-16954	VBD	denotes	were
T4546	16955-16963	RB	denotes	directly
T4544	16964-16971	VBN	denotes	excised
T4547	16972-16976	IN	denotes	from
T4548	16977-16984	NN	denotes	agarose
T4549	16985-16988	NN	denotes	gel
T4550	16989-16992	CC	denotes	and
T4551	16993-17002	VBN	denotes	sequenced
T4552	17003-17005	IN	denotes	by
T4553	17006-17008	DT	denotes	an
T4555	17009-17015	NN	denotes	ABI377
T4556	17016-17025	JJ	denotes	automatic
T4554	17026-17035	NN	denotes	sequencer
T4557	17035-17036	.	denotes	.
T4558	17036-17167	sentence	denotes	The sequence was further confirmed using a forward primer from newly identified sequence and a reverse primer from known sequence.
T4559	17037-17040	DT	denotes	The
T4560	17041-17049	NN	denotes	sequence
T4562	17050-17053	VBD	denotes	was
T4563	17054-17061	RB	denotes	further
T4561	17062-17071	VBN	denotes	confirmed
T4564	17072-17077	VBG	denotes	using
T4565	17078-17079	DT	denotes	a
T4567	17080-17087	JJ	denotes	forward
T4566	17088-17094	NN	denotes	primer
T4568	17095-17099	IN	denotes	from
T4569	17100-17105	RB	denotes	newly
T4570	17106-17116	VBN	denotes	identified
T4571	17117-17125	NN	denotes	sequence
T4572	17126-17129	CC	denotes	and
T4573	17130-17131	DT	denotes	a
T4575	17132-17139	JJ	denotes	reverse
T4574	17140-17146	NN	denotes	primer
T4576	17147-17151	IN	denotes	from
T4577	17152-17157	JJ	denotes	known
T4578	17158-17166	NN	denotes	sequence
T4579	17166-17167	.	denotes	.
T4580	17167-17307	sentence	denotes	Once most of the coding sequences were identified, partial sequence of exon 1 and the 5' UTR sequences were obtained by BAC DNA sequencing.
T4581	17168-17172	IN	denotes	Once
T4583	17173-17177	JJS	denotes	most
T4584	17178-17180	IN	denotes	of
T4585	17181-17184	DT	denotes	the
T4587	17185-17191	VBG	denotes	coding
T4586	17192-17201	NNS	denotes	sequences
T4588	17202-17206	VBD	denotes	were
T4582	17207-17217	VBN	denotes	identified
T4590	17217-17219	,	denotes	,
T4591	17219-17226	JJ	denotes	partial
T4592	17227-17235	NN	denotes	sequence
T4593	17236-17238	IN	denotes	of
T4594	17239-17243	NN	denotes	exon
T4596	17244-17245	CD	denotes	1
T4597	17246-17249	CC	denotes	and
T4598	17250-17253	DT	denotes	the
T4600	17254-17255	CD	denotes	5
T4601	17255-17256	SYM	denotes	'
T4599	17257-17260	NN	denotes	UTR
T4595	17261-17270	NNS	denotes	sequences
T4602	17271-17275	VBD	denotes	were
T4589	17276-17284	VBN	denotes	obtained
T4603	17285-17287	IN	denotes	by
T4604	17288-17291	NN	denotes	BAC
T4606	17292-17295	NN	denotes	DNA
T4605	17296-17306	NN	denotes	sequencing
T4607	17306-17307	.	denotes	.
T4647	17309-17317	NNP	denotes	Northern
T4648	17318-17322	NN	denotes	blot
T4649	17323-17331	NNS	denotes	analyses
T4650	17331-17436	sentence	denotes	Multiple Choice Northern Blot filters containing 12 different mouse tissues were purchased from Origene.
T4651	17332-17340	JJ	denotes	Multiple
T4653	17341-17347	NN	denotes	Choice
T4654	17348-17356	NNP	denotes	Northern
T4655	17357-17361	NN	denotes	Blot
T4652	17362-17369	NNS	denotes	filters
T4657	17370-17380	VBG	denotes	containing
T4658	17381-17383	CD	denotes	12
T4660	17384-17393	JJ	denotes	different
T4661	17394-17399	NN	denotes	mouse
T4659	17400-17407	NNS	denotes	tissues
T4662	17408-17412	VBD	denotes	were
T4656	17413-17422	VBN	denotes	purchased
T4663	17423-17427	IN	denotes	from
T4664	17428-17435	NNP	denotes	Origene
T4665	17435-17436	.	denotes	.
T4666	17436-17647	sentence	denotes	The filters were probed for each Acdp gene with a PCR fragment (around 350 bp) from last exon and the 3' untranslated region labeled with α-32P dCTP using the random primer extension system (Life Technologies).
T4667	17437-17440	DT	denotes	The
T4668	17441-17448	NNS	denotes	filters
T4670	17449-17453	VBD	denotes	were
T4669	17454-17460	VBN	denotes	probed
T4671	17461-17464	IN	denotes	for
T4672	17465-17469	DT	denotes	each
T4674	17470-17474	NN	denotes	Acdp
T4673	17475-17479	NN	denotes	gene
T4675	17480-17484	IN	denotes	with
T4676	17485-17486	DT	denotes	a
T4678	17487-17490	NN	denotes	PCR
T4677	17491-17499	NN	denotes	fragment
T4679	17500-17501	-LRB-	denotes	(
T4681	17501-17507	IN	denotes	around
T4682	17508-17511	CD	denotes	350
T4680	17512-17514	NN	denotes	bp
T4683	17514-17515	-RRB-	denotes	)
T4684	17516-17520	IN	denotes	from
T4685	17521-17525	JJ	denotes	last
T4686	17526-17530	NN	denotes	exon
T4687	17531-17534	CC	denotes	and
T4688	17535-17538	DT	denotes	the
T4689	17539-17540	CD	denotes	3
T4691	17540-17541	SYM	denotes	'
T4692	17542-17554	JJ	denotes	untranslated
T4690	17555-17561	NN	denotes	region
T4693	17562-17569	VBN	denotes	labeled
T4694	17570-17574	IN	denotes	with
T4695	17575-17576	NN	denotes	α
T4697	17576-17577	HYPH	denotes	-
T4696	17577-17580	NN	denotes	32P
T4698	17581-17585	NN	denotes	dCTP
T4699	17586-17591	VBG	denotes	using
T4700	17592-17595	DT	denotes	the
T4702	17596-17602	JJ	denotes	random
T4703	17603-17609	NN	denotes	primer
T4704	17610-17619	NN	denotes	extension
T4701	17620-17626	NN	denotes	system
T4705	17627-17628	-LRB-	denotes	(
T4707	17628-17632	NNP	denotes	Life
T4706	17633-17645	NNP	denotes	Technologies
T4708	17645-17646	-RRB-	denotes	)
T4709	17646-17647	.	denotes	.
T4710	17647-17690	sentence	denotes	Hybridizations were carried out overnight.
T4711	17648-17662	NNS	denotes	Hybridizations
T4713	17663-17667	VBD	denotes	were
T4712	17668-17675	VBN	denotes	carried
T4714	17676-17679	RP	denotes	out
T4715	17680-17689	RB	denotes	overnight
T4716	17689-17690	.	denotes	.
T4717	17690-17866	sentence	denotes	The filters were washed twice with washing buffer I (2×SSC, 0.1% SDS) at 42°C for 15-min, and then washed twice with washing buffer II (0.25×SSC, 0.1% SDS) at 65°C for 15-min.
T4718	17691-17694	DT	denotes	The
T4719	17695-17702	NNS	denotes	filters
T4721	17703-17707	VBD	denotes	were
T4720	17708-17714	VBN	denotes	washed
T4722	17715-17720	RB	denotes	twice
T4723	17721-17725	IN	denotes	with
T4724	17726-17733	VBG	denotes	washing
T4725	17734-17740	NN	denotes	buffer
T4726	17741-17742	CD	denotes	I
T4727	17743-17744	-LRB-	denotes	(
T4729	17744-17745	CD	denotes	2
T4731	17745-17746	SYM	denotes	×
T4730	17746-17749	NN	denotes	SSC
T4732	17749-17751	,	denotes	,
T4733	17751-17754	CD	denotes	0.1
T4734	17754-17755	NN	denotes	%
T4728	17756-17759	NN	denotes	SDS
T4735	17759-17760	-RRB-	denotes	)
T4736	17761-17763	IN	denotes	at
T4737	17764-17766	CD	denotes	42
T4738	17766-17768	NN	denotes	°C
T4739	17769-17772	IN	denotes	for
T4740	17773-17775	CD	denotes	15
T4742	17775-17776	HYPH	denotes	-
T4741	17776-17779	NN	denotes	min
T4743	17779-17781	,	denotes	,
T4744	17781-17784	CC	denotes	and
T4745	17785-17789	RB	denotes	then
T4746	17790-17796	VBD	denotes	washed
T4747	17797-17802	RB	denotes	twice
T4748	17803-17807	IN	denotes	with
T4749	17808-17815	NN	denotes	washing
T4750	17816-17822	NN	denotes	buffer
T4751	17823-17825	CD	denotes	II
T4752	17826-17827	-LRB-	denotes	(
T4754	17827-17831	CD	denotes	0.25
T4756	17831-17832	SYM	denotes	×
T4755	17832-17835	NN	denotes	SSC
T4757	17835-17837	,	denotes	,
T4758	17837-17840	CD	denotes	0.1
T4759	17840-17841	NN	denotes	%
T4753	17842-17845	NN	denotes	SDS
T4760	17845-17846	-RRB-	denotes	)
T4761	17847-17849	IN	denotes	at
T4762	17850-17852	CD	denotes	65
T4763	17852-17854	NN	denotes	°C
T4764	17855-17858	IN	denotes	for
T4765	17859-17861	CD	denotes	15
T4767	17861-17862	HYPH	denotes	-
T4766	17862-17865	NN	denotes	min
T4768	17865-17866	.	denotes	.
T4769	17866-17940	sentence	denotes	Washed filters were exposed to X-ray films for overnight or longer [3,4].
T4770	17867-17873	JJ	denotes	Washed
T4771	17874-17881	NNS	denotes	filters
T4773	17882-17886	VBD	denotes	were
T4772	17887-17894	VBN	denotes	exposed
T4774	17895-17897	IN	denotes	to
T4775	17898-17903	NN	denotes	X-ray
T4776	17904-17909	NNS	denotes	films
T4777	17910-17913	IN	denotes	for
T4778	17914-17923	NN	denotes	overnight
T4779	17924-17926	CC	denotes	or
T4780	17927-17933	RBR	denotes	longer
T4781	17934-17935	-LRB-	denotes	[
T4783	17935-17936	CD	denotes	3
T4784	17936-17937	,	denotes	,
T4782	17937-17938	CD	denotes	4
T4785	17938-17939	-RRB-	denotes	]
T4786	17939-17940	.	denotes	.
T4823	17942-17950	NN	denotes	Antibody
T4824	17951-17961	NN	denotes	production
T4825	17962-17965	CC	denotes	and
T4826	17966-17973	NNP	denotes	Western
T4827	17974-17978	NN	denotes	blot
T4828	17979-17987	NNS	denotes	analyses
T4829	17987-18213	sentence	denotes	Peptides linked to KLH (keyhole limpet hemacyanin) from Acdp1 N- and C-terminals and the conserved domain (ACD) were used for generation of antibodies specifically for Acdp1 and all Acdp members as reported, respectively [5].
T4830	17988-17996	NNS	denotes	Peptides
T4832	17997-18003	VBN	denotes	linked
T4833	18004-18006	IN	denotes	to
T4834	18007-18010	NN	denotes	KLH
T4835	18011-18012	-LRB-	denotes	(
T4836	18012-18019	NN	denotes	keyhole
T4838	18020-18026	NN	denotes	limpet
T4837	18027-18037	NN	denotes	hemacyanin
T4839	18037-18038	-RRB-	denotes	)
T4840	18039-18043	IN	denotes	from
T4841	18044-18049	NN	denotes	Acdp1
T4843	18050-18051	NN	denotes	N
T4844	18051-18052	HYPH	denotes	-
T4845	18053-18056	CC	denotes	and
T4846	18057-18058	NN	denotes	C
T4847	18058-18059	HYPH	denotes	-
T4842	18059-18068	NNS	denotes	terminals
T4848	18069-18072	CC	denotes	and
T4849	18073-18076	DT	denotes	the
T4851	18077-18086	VBN	denotes	conserved
T4850	18087-18093	NN	denotes	domain
T4852	18094-18095	-LRB-	denotes	(
T4853	18095-18098	NN	denotes	ACD
T4854	18098-18099	-RRB-	denotes	)
T4855	18100-18104	VBD	denotes	were
T4831	18105-18109	VBN	denotes	used
T4856	18110-18113	IN	denotes	for
T4857	18114-18124	NN	denotes	generation
T4858	18125-18127	IN	denotes	of
T4859	18128-18138	NNS	denotes	antibodies
T4860	18139-18151	RB	denotes	specifically
T4861	18152-18155	IN	denotes	for
T4862	18156-18161	NN	denotes	Acdp1
T4863	18162-18165	CC	denotes	and
T4864	18166-18169	DT	denotes	all
T4866	18170-18174	NN	denotes	Acdp
T4865	18175-18182	NNS	denotes	members
T4867	18183-18185	IN	denotes	as
T4868	18186-18194	VBN	denotes	reported
T4869	18194-18196	,	denotes	,
T4870	18196-18208	RB	denotes	respectively
T4871	18209-18210	-LRB-	denotes	[
T4872	18210-18211	CD	denotes	5
T4873	18211-18212	-RRB-	denotes	]
T4874	18212-18213	.	denotes	.
T4875	18213-18292	sentence	denotes	Western blots were carried out Using ECL (PIERCE) as described previously [1].
T4876	18214-18221	NNP	denotes	Western
T4877	18222-18227	NNS	denotes	blots
T4879	18228-18232	VBD	denotes	were
T4878	18233-18240	VBN	denotes	carried
T4880	18241-18244	RP	denotes	out
T4881	18245-18250	VBG	denotes	Using
T4882	18251-18254	NN	denotes	ECL
T4883	18255-18256	-LRB-	denotes	(
T4884	18256-18262	NN	denotes	PIERCE
T4885	18262-18263	-RRB-	denotes	)
T4886	18264-18266	IN	denotes	as
T4887	18267-18276	VBN	denotes	described
T4888	18277-18287	RB	denotes	previously
T4889	18288-18289	-LRB-	denotes	[
T4890	18289-18290	CD	denotes	1
T4891	18290-18291	-RRB-	denotes	]
T4892	18291-18292	.	denotes	.
T4893	18292-18454	sentence	denotes	The membranes were washed extensively after incubation with primary and secondary antibodies and were then developed with X-ray films with optimal exposure time.
T4894	18293-18296	DT	denotes	The
T4895	18297-18306	NNS	denotes	membranes
T4897	18307-18311	VBD	denotes	were
T4896	18312-18318	VBN	denotes	washed
T4898	18319-18330	RB	denotes	extensively
T4899	18331-18336	IN	denotes	after
T4900	18337-18347	NN	denotes	incubation
T4901	18348-18352	IN	denotes	with
T4902	18353-18360	JJ	denotes	primary
T4904	18361-18364	CC	denotes	and
T4905	18365-18374	JJ	denotes	secondary
T4903	18375-18385	NNS	denotes	antibodies
T4906	18386-18389	CC	denotes	and
T4907	18390-18394	VBD	denotes	were
T4909	18395-18399	RB	denotes	then
T4908	18400-18409	VBN	denotes	developed
T4910	18410-18414	IN	denotes	with
T4911	18415-18420	NN	denotes	X-ray
T4912	18421-18426	NNS	denotes	films
T4913	18427-18431	IN	denotes	with
T4914	18432-18439	JJ	denotes	optimal
T4916	18440-18448	NN	denotes	exposure
T4915	18449-18453	NN	denotes	time
T4917	18453-18454	.	denotes	.
T4941	18456-18466	NN	denotes	Chromosome
T4942	18467-18479	NN	denotes	localization
T4943	18479-18600	sentence	denotes	The T31 mouse radiation hybrid panel from Research Genetics was used to map the chromosome location of each Acdp member.
T4944	18480-18483	DT	denotes	The
T4946	18484-18487	NN	denotes	T31
T4947	18488-18493	NN	denotes	mouse
T4948	18494-18503	NN	denotes	radiation
T4949	18504-18510	NN	denotes	hybrid
T4945	18511-18516	NN	denotes	panel
T4951	18517-18521	IN	denotes	from
T4952	18522-18530	NNP	denotes	Research
T4953	18531-18539	NNP	denotes	Genetics
T4954	18540-18543	VBD	denotes	was
T4950	18544-18548	VBN	denotes	used
T4955	18549-18551	TO	denotes	to
T4956	18552-18555	VB	denotes	map
T4957	18556-18559	DT	denotes	the
T4959	18560-18570	NN	denotes	chromosome
T4958	18571-18579	NN	denotes	location
T4960	18580-18582	IN	denotes	of
T4961	18583-18587	DT	denotes	each
T4963	18588-18592	NN	denotes	Acdp
T4962	18593-18599	NN	denotes	member
T4964	18599-18600	.	denotes	.
T4965	18600-18724	sentence	denotes	Primers from 3' UTR of each Acdp member were used to amplify the 100 radiation hybrid clones representing the mouse genome.
T4966	18601-18608	NNS	denotes	Primers
T4968	18609-18613	IN	denotes	from
T4969	18614-18615	CD	denotes	3
T4971	18615-18616	SYM	denotes	'
T4970	18617-18620	NN	denotes	UTR
T4972	18621-18623	IN	denotes	of
T4973	18624-18628	DT	denotes	each
T4975	18629-18633	NN	denotes	Acdp
T4974	18634-18640	NN	denotes	member
T4976	18641-18645	VBD	denotes	were
T4967	18646-18650	VBN	denotes	used
T4977	18651-18653	TO	denotes	to
T4978	18654-18661	VB	denotes	amplify
T4979	18662-18665	DT	denotes	the
T4981	18666-18669	CD	denotes	100
T4982	18670-18679	NN	denotes	radiation
T4983	18680-18686	JJ	denotes	hybrid
T4980	18687-18693	NNS	denotes	clones
T4984	18694-18706	VBG	denotes	representing
T4985	18707-18710	DT	denotes	the
T4987	18711-18716	NN	denotes	mouse
T4986	18717-18723	NN	denotes	genome
T4988	18723-18724	.	denotes	.
T4989	18724-18820	sentence	denotes	The data were submitted to the Jackson Laboratory Mouse Radiation Hybrid Database for analysis.
T4990	18725-18728	DT	denotes	The
T4991	18729-18733	NNS	denotes	data
T4993	18734-18738	VBD	denotes	were
T4992	18739-18748	VBN	denotes	submitted
T4994	18749-18751	IN	denotes	to
T4995	18752-18755	DT	denotes	the
T4997	18756-18763	NNP	denotes	Jackson
T4998	18764-18774	NNP	denotes	Laboratory
T4999	18775-18780	NNP	denotes	Mouse
T5001	18781-18790	NNP	denotes	Radiation
T5000	18791-18797	NNP	denotes	Hybrid
T4996	18798-18806	NNP	denotes	Database
T5002	18807-18810	IN	denotes	for
T5003	18811-18819	NN	denotes	analysis
T5004	18819-18820	.	denotes	.
T5023	18822-18830	NN	denotes	Sequence
T5024	18831-18839	NNS	denotes	analyses
T5025	18839-18914	sentence	denotes	Sequence assembly was performed with program Sequencher (Gene Codes Corp).
T5026	18840-18848	NN	denotes	Sequence
T5027	18849-18857	NN	denotes	assembly
T5029	18858-18861	VBD	denotes	was
T5028	18862-18871	VBN	denotes	performed
T5030	18872-18876	IN	denotes	with
T5031	18877-18884	NN	denotes	program
T5032	18885-18895	NN	denotes	Sequencher
T5033	18896-18897	-LRB-	denotes	(
T5035	18897-18901	NNP	denotes	Gene
T5036	18902-18907	NNPS	denotes	Codes
T5034	18908-18912	NNP	denotes	Corp
T5037	18912-18913	-RRB-	denotes	)
T5038	18913-18914	.	denotes	.
T5039	18914-19017	sentence	denotes	Protein and DNA homology searches were carried out with tblastn, tblastx, blastp and blastn programs .
T5040	18915-18922	NN	denotes	Protein
T5042	18923-18926	CC	denotes	and
T5043	18927-18930	NN	denotes	DNA
T5041	18931-18939	NN	denotes	homology
T5044	18940-18948	NNS	denotes	searches
T5046	18949-18953	VBD	denotes	were
T5045	18954-18961	VBN	denotes	carried
T5047	18962-18965	RP	denotes	out
T5048	18966-18970	IN	denotes	with
T5049	18971-18978	NN	denotes	tblastn
T5051	18978-18980	,	denotes	,
T5052	18980-18987	NN	denotes	tblastx
T5053	18987-18989	,	denotes	,
T5054	18989-18995	NN	denotes	blastp
T5055	18996-18999	CC	denotes	and
T5056	19000-19006	NN	denotes	blastn
T5050	19007-19015	NNS	denotes	programs
T5057	19016-19017	.	denotes	.
T5058	19017-19108	sentence	denotes	Multiple sequence alignments were performed with GeneDoc and pairwise sequence alignment .
T5059	19018-19026	JJ	denotes	Multiple
T5061	19027-19035	NN	denotes	sequence
T5060	19036-19046	NNS	denotes	alignments
T5063	19047-19051	VBD	denotes	were
T5062	19052-19061	VBN	denotes	performed
T5064	19062-19066	IN	denotes	with
T5065	19067-19074	NN	denotes	GeneDoc
T5067	19075-19078	CC	denotes	and
T5068	19079-19087	JJ	denotes	pairwise
T5069	19088-19096	NN	denotes	sequence
T5066	19097-19106	NN	denotes	alignment
T5070	19107-19108	.	denotes	.
T5071	19108-19276	sentence	denotes	Multiple programs including BCM Search Launcher , ProfileScan , sequence motif search , ExPASy and 3Dpssm were used for searching sequence features of known protein.
T5072	19109-19117	JJ	denotes	Multiple
T5073	19118-19126	NNS	denotes	programs
T5075	19127-19136	VBG	denotes	including
T5076	19137-19140	NN	denotes	BCM
T5078	19141-19147	NN	denotes	Search
T5077	19148-19156	NN	denotes	Launcher
T5079	19157-19159	,	denotes	,
T5080	19159-19170	NN	denotes	ProfileScan
T5081	19171-19173	,	denotes	,
T5082	19173-19181	NN	denotes	sequence
T5084	19182-19187	NN	denotes	motif
T5083	19188-19194	NN	denotes	search
T5085	19195-19197	,	denotes	,
T5086	19197-19203	NN	denotes	ExPASy
T5087	19205-19208	CC	denotes	and
T5088	19209-19215	NN	denotes	3Dpssm
T5089	19217-19221	VBD	denotes	were
T5074	19222-19226	VBN	denotes	used
T5090	19227-19230	IN	denotes	for
T5091	19231-19240	VBG	denotes	searching
T5092	19241-19249	NN	denotes	sequence
T5093	19250-19258	NNS	denotes	features
T5094	19259-19261	IN	denotes	of
T5095	19262-19267	JJ	denotes	known
T5096	19268-19275	NN	denotes	protein
T5097	19275-19276	.	denotes	.
T5098	19276-19430	sentence	denotes	Phylogenetic tree was constructed by Clustalw program (version 1.81) using UPGMA (Unweighted Pair Group Method using Arithmetic averages) algorithm [14].
T5099	19277-19289	JJ	denotes	Phylogenetic
T5100	19290-19294	NN	denotes	tree
T5102	19295-19298	VBD	denotes	was
T5101	19299-19310	VBN	denotes	constructed
T5103	19311-19313	IN	denotes	by
T5104	19314-19322	NN	denotes	Clustalw
T5105	19323-19330	NN	denotes	program
T5106	19331-19332	-LRB-	denotes	(
T5107	19332-19339	NN	denotes	version
T5108	19340-19344	CD	denotes	1.81
T5109	19344-19345	-RRB-	denotes	)
T5110	19346-19351	VBG	denotes	using
T5111	19352-19357	NN	denotes	UPGMA
T5113	19358-19359	-LRB-	denotes	(
T5114	19359-19369	JJ	denotes	Unweighted
T5116	19370-19374	NN	denotes	Pair
T5117	19375-19380	NN	denotes	Group
T5115	19381-19387	NN	denotes	Method
T5118	19388-19393	VBG	denotes	using
T5119	19394-19404	NN	denotes	Arithmetic
T5120	19405-19413	NNS	denotes	averages
T5121	19413-19414	-RRB-	denotes	)
T5112	19415-19424	NN	denotes	algorithm
T5122	19425-19426	-LRB-	denotes	[
T5123	19426-19428	CD	denotes	14
T5124	19428-19429	-RRB-	denotes	]
T5125	19429-19430	.	denotes	.
T5264	19432-19440	JJ	denotes	Neuronal
T5266	19441-19445	NN	denotes	cell
T5265	19446-19457	NN	denotes	preparation
T5267	19458-19461	CC	denotes	and
T5268	19462-19477	NN	denotes	immunostanining
T5269	19477-19547	sentence	denotes	Hippocampal neuron cultures were prepared as previously reported [6].
T5270	19478-19489	JJ	denotes	Hippocampal
T5272	19490-19496	NN	denotes	neuron
T5271	19497-19505	NNS	denotes	cultures
T5274	19506-19510	VBD	denotes	were
T5273	19511-19519	VBN	denotes	prepared
T5275	19520-19522	IN	denotes	as
T5277	19523-19533	RB	denotes	previously
T5276	19534-19542	VBN	denotes	reported
T5278	19543-19544	-LRB-	denotes	[
T5279	19544-19545	CD	denotes	6
T5280	19545-19546	-RRB-	denotes	]
T5281	19546-19547	.	denotes	.
T5282	19547-19634	sentence	denotes	In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero.
T5283	19548-19550	IN	denotes	In
T5285	19551-19556	NN	denotes	brief
T5286	19556-19558	,	denotes	,
T5287	19558-19561	DT	denotes	the
T5288	19562-19575	NNS	denotes	hippocampuses
T5289	19576-19580	VBD	denotes	were
T5284	19581-19590	VBN	denotes	dissected
T5290	19591-19594	RP	denotes	out
T5291	19595-19599	IN	denotes	from
T5292	19600-19605	NN	denotes	mouse
T5293	19606-19613	NNS	denotes	embryos
T5294	19614-19616	IN	denotes	at
T5295	19617-19619	CD	denotes	16
T5296	19620-19624	NNS	denotes	days
T5297	19625-19627	FW	denotes	in
T5298	19628-19633	FW	denotes	utero
T5299	19633-19634	.	denotes	.
T5300	19634-19811	sentence	denotes	The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies).
T5301	19635-19638	DT	denotes	The
T5302	19639-19646	NNS	denotes	tissues
T5304	19647-19651	VBD	denotes	were
T5305	19652-19656	RB	denotes	then
T5303	19657-19666	VBN	denotes	incubated
T5306	19667-19670	IN	denotes	for
T5307	19671-19673	CD	denotes	20
T5308	19674-19677	NN	denotes	min
T5309	19678-19680	IN	denotes	at
T5310	19681-19683	CD	denotes	37
T5311	19683-19685	NN	denotes	°C
T5312	19686-19688	IN	denotes	in
T5313	19689-19692	NN	denotes	MEM
T5314	19693-19694	-LRB-	denotes	(
T5315	19694-19701	JJ	denotes	minimum
T5317	19702-19711	JJ	denotes	essential
T5316	19712-19718	NN	denotes	medium
T5318	19718-19719	-RRB-	denotes	)
T5319	19720-19728	VBN	denotes	modified
T5320	19729-19732	IN	denotes	for
T5321	19733-19743	NN	denotes	suspension
T5322	19744-19751	NN	denotes	culture
T5323	19752-19753	-LRB-	denotes	(
T5325	19753-19757	NNP	denotes	Life
T5324	19758-19770	NNP	denotes	Technologies
T5326	19770-19771	-RRB-	denotes	)
T5327	19772-19776	CC	denotes	plus
T5328	19777-19781	CD	denotes	0.25
T5329	19781-19782	NN	denotes	%
T5330	19783-19790	NN	denotes	trypsin
T5331	19791-19792	-LRB-	denotes	(
T5333	19792-19796	NNP	denotes	Life
T5332	19797-19809	NNP	denotes	Technologies
T5334	19809-19810	-RRB-	denotes	)
T5335	19810-19811	.	denotes	.
T5336	19811-19971	sentence	denotes	The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below.
T5337	19812-19815	DT	denotes	The
T5339	19816-19827	JJ	denotes	dissociated
T5340	19828-19839	JJ	denotes	hippocampal
T5338	19840-19847	NNS	denotes	neurons
T5342	19848-19852	VBD	denotes	were
T5341	19853-19859	VBN	denotes	plated
T5343	19860-19862	IN	denotes	on
T5344	19863-19868	NN	denotes	glass
T5345	19869-19879	NNS	denotes	coverslips
T5346	19880-19886	VBN	denotes	coated
T5347	19887-19891	IN	denotes	with
T5348	19892-19893	DT	denotes	a
T5350	19894-19903	JJ	denotes	confluent
T5349	19904-19913	NN	denotes	monolayer
T5351	19914-19916	IN	denotes	of
T5352	19917-19922	NN	denotes	mouse
T5354	19923-19931	JJ	denotes	cortical
T5353	19932-19942	NNS	denotes	astrocytes
T5355	19943-19951	VBN	denotes	obtained
T5356	19952-19954	IN	denotes	as
T5357	19955-19964	VBN	denotes	described
T5358	19965-19970	RB	denotes	below
T5359	19970-19971	.	denotes	.
T5360	19971-20047	sentence	denotes	The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2.
T5361	19972-19975	DT	denotes	The
T5362	19976-19983	NNS	denotes	neurons
T5364	19984-19988	VBD	denotes	were
T5363	19989-19999	VBN	denotes	maintained
T5365	20000-20002	IN	denotes	at
T5366	20003-20005	CD	denotes	37
T5367	20005-20007	NN	denotes	°C
T5368	20008-20010	IN	denotes	in
T5369	20011-20012	DT	denotes	a
T5371	20013-20023	JJ	denotes	humidified
T5370	20024-20034	NN	denotes	atmosphere
T5372	20035-20039	IN	denotes	with
T5373	20040-20041	CD	denotes	5
T5374	20041-20042	NN	denotes	%
T5375	20043-20046	NN	denotes	CO2
T5376	20046-20047	.	denotes	.
T5377	20047-20200	sentence	denotes	Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent.
T5378	20048-20056	JJ	denotes	Cortical
T5379	20057-20067	NNS	denotes	astrocytes
T5381	20068-20079	VBN	denotes	dissociated
T5382	20080-20084	IN	denotes	from
T5383	20085-20092	JJ	denotes	newborn
T5384	20093-20098	NN	denotes	mouse
T5385	20099-20107	NNS	denotes	cortices
T5386	20108-20112	VBD	denotes	were
T5380	20113-20118	VBN	denotes	grown
T5387	20119-20121	IN	denotes	in
T5388	20122-20129	NN	denotes	culture
T5389	20130-20136	NNS	denotes	flasks
T5390	20137-20139	IN	denotes	at
T5391	20140-20142	CD	denotes	37
T5392	20142-20144	NN	denotes	°C
T5393	20145-20147	IN	denotes	in
T5394	20148-20149	DT	denotes	a
T5396	20150-20160	JJ	denotes	humidified
T5395	20161-20171	NN	denotes	atmosphere
T5397	20172-20176	IN	denotes	with
T5398	20177-20178	CD	denotes	5
T5399	20178-20179	NN	denotes	%
T5400	20180-20183	NN	denotes	CO2
T5401	20184-20189	IN	denotes	until
T5402	20190-20199	JJ	denotes	confluent
T5403	20199-20200	.	denotes	.
T5404	20200-20309	sentence	denotes	The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C.
T5405	20201-20204	DT	denotes	The
T5406	20205-20210	NNS	denotes	cells
T5408	20211-20215	VBD	denotes	were
T5407	20216-20223	VBN	denotes	exposed
T5409	20224-20226	IN	denotes	to
T5410	20227-20229	CD	denotes	10
T5412	20229-20230	SYM	denotes	-
T5411	20230-20231	CD	denotes	5
T5413	20232-20233	NN	denotes	M
T5415	20234-20242	NN	denotes	cytosine
T5414	20243-20254	NN	denotes	arabinoside
T5416	20255-20256	-LRB-	denotes	(
T5417	20256-20261	NNP	denotes	Sigma
T5418	20261-20262	-RRB-	denotes	)
T5419	20263-20266	CC	denotes	and
T5420	20267-20275	VBN	denotes	cultured
T5421	20276-20279	IN	denotes	for
T5422	20280-20290	JJ	denotes	additional
T5424	20291-20293	CD	denotes	12
T5426	20293-20294	SYM	denotes	–
T5425	20294-20296	CD	denotes	24
T5423	20297-20300	NNS	denotes	hrs
T5427	20301-20303	IN	denotes	at
T5428	20304-20306	CD	denotes	37
T5429	20306-20308	NN	denotes	°C
T5430	20308-20309	.	denotes	.
T5431	20309-20401	sentence	denotes	After remove of the media with cellular debris, the cells were used for coating coverslips.
T5432	20310-20315	IN	denotes	After
T5434	20316-20322	NN	denotes	remove
T5435	20323-20325	IN	denotes	of
T5436	20326-20329	DT	denotes	the
T5437	20330-20335	NNS	denotes	media
T5438	20336-20340	IN	denotes	with
T5439	20341-20349	JJ	denotes	cellular
T5440	20350-20356	NN	denotes	debris
T5441	20356-20358	,	denotes	,
T5442	20358-20361	DT	denotes	the
T5443	20362-20367	NNS	denotes	cells
T5444	20368-20372	VBD	denotes	were
T5433	20373-20377	VBN	denotes	used
T5445	20378-20381	IN	denotes	for
T5446	20382-20389	VBG	denotes	coating
T5447	20390-20400	NNS	denotes	coverslips
T5448	20400-20401	.	denotes	.
T5449	20401-20623	sentence	denotes	For immunostaining, neuronal cells on the coverslips were first fixed in PBS containing 4% paraformaldehyde (PFA) for 12 hrs at 4°C and then incubated in a solution containing 4% PFA and 0.4% Triton X-100 at 4°C for 1 hr.
T5450	20402-20405	IN	denotes	For
T5452	20406-20420	NN	denotes	immunostaining
T5453	20420-20422	,	denotes	,
T5454	20422-20430	JJ	denotes	neuronal
T5455	20431-20436	NNS	denotes	cells
T5456	20437-20439	IN	denotes	on
T5457	20440-20443	DT	denotes	the
T5458	20444-20454	NNS	denotes	coverslips
T5459	20455-20459	VBD	denotes	were
T5460	20460-20465	RB	denotes	first
T5451	20466-20471	VBN	denotes	fixed
T5461	20472-20474	IN	denotes	in
T5462	20475-20478	NN	denotes	PBS
T5463	20479-20489	VBG	denotes	containing
T5464	20490-20491	CD	denotes	4
T5465	20491-20492	NN	denotes	%
T5466	20493-20509	NN	denotes	paraformaldehyde
T5467	20510-20511	-LRB-	denotes	(
T5468	20511-20514	NN	denotes	PFA
T5469	20514-20515	-RRB-	denotes	)
T5470	20516-20519	IN	denotes	for
T5471	20520-20522	CD	denotes	12
T5472	20523-20526	NNS	denotes	hrs
T5473	20527-20529	IN	denotes	at
T5474	20530-20531	CD	denotes	4
T5475	20531-20533	NN	denotes	°C
T5476	20534-20537	CC	denotes	and
T5477	20538-20542	RB	denotes	then
T5478	20543-20552	VBN	denotes	incubated
T5479	20553-20555	IN	denotes	in
T5480	20556-20557	DT	denotes	a
T5481	20558-20566	NN	denotes	solution
T5482	20567-20577	VBG	denotes	containing
T5483	20578-20579	CD	denotes	4
T5484	20579-20580	NN	denotes	%
T5485	20581-20584	NN	denotes	PFA
T5486	20585-20588	CC	denotes	and
T5487	20589-20592	CD	denotes	0.4
T5488	20592-20593	NN	denotes	%
T5490	20594-20600	NN	denotes	Triton
T5491	20601-20602	NN	denotes	X
T5492	20602-20603	HYPH	denotes	-
T5489	20603-20606	NN	denotes	100
T5493	20607-20609	IN	denotes	at
T5494	20610-20611	CD	denotes	4
T5495	20611-20613	NN	denotes	°C
T5496	20614-20617	IN	denotes	for
T5497	20618-20619	CD	denotes	1
T5498	20620-20622	NN	denotes	hr
T5499	20622-20623	.	denotes	.
T5500	20623-20842	sentence	denotes	After washing with PBS three times, the cells were incubated with a blocking solution containing 1:30 normal goat serum, and subsequently incubated with a rabbit polyclonal anti-ACDP antibody (1:3000) overnight at 4°C.
T5501	20624-20629	IN	denotes	After
T5503	20630-20637	VBG	denotes	washing
T5504	20638-20642	IN	denotes	with
T5505	20643-20646	NN	denotes	PBS
T5506	20647-20652	CD	denotes	three
T5507	20653-20658	NNS	denotes	times
T5508	20658-20660	,	denotes	,
T5509	20660-20663	DT	denotes	the
T5510	20664-20669	NNS	denotes	cells
T5511	20670-20674	VBD	denotes	were
T5502	20675-20684	VBN	denotes	incubated
T5512	20685-20689	IN	denotes	with
T5513	20690-20691	DT	denotes	a
T5515	20692-20700	VBG	denotes	blocking
T5514	20701-20709	NN	denotes	solution
T5516	20710-20720	VBG	denotes	containing
T5517	20721-20722	CD	denotes	1
T5519	20722-20723	SYM	denotes	:
T5520	20723-20725	CD	denotes	30
T5521	20726-20732	JJ	denotes	normal
T5522	20733-20737	NN	denotes	goat
T5518	20738-20743	NN	denotes	serum
T5523	20743-20745	,	denotes	,
T5524	20745-20748	CC	denotes	and
T5525	20749-20761	RB	denotes	subsequently
T5526	20762-20771	VBN	denotes	incubated
T5527	20772-20776	IN	denotes	with
T5528	20777-20778	DT	denotes	a
T5530	20779-20785	NN	denotes	rabbit
T5531	20786-20796	JJ	denotes	polyclonal
T5532	20797-20806	JJ	denotes	anti-ACDP
T5529	20807-20815	NN	denotes	antibody
T5533	20816-20817	-LRB-	denotes	(
T5534	20817-20818	CD	denotes	1
T5535	20818-20819	SYM	denotes	:
T5536	20819-20823	CD	denotes	3000
T5537	20823-20824	-RRB-	denotes	)
T5538	20825-20834	RB	denotes	overnight
T5539	20835-20837	IN	denotes	at
T5540	20838-20839	CD	denotes	4
T5541	20839-20841	NN	denotes	°C
T5542	20841-20842	.	denotes	.
T5543	20842-21058	sentence	denotes	After extensive washing with 1% goat serum PBS solution, the cells were incubated with an Alex 488 conjugated secondary antibody (1:100 in 1% goat serum PBS solution, Molecular Probes) for 3 hrs at room temperature.
T5544	20843-20848	IN	denotes	After
T5546	20849-20858	JJ	denotes	extensive
T5547	20859-20866	NN	denotes	washing
T5548	20867-20871	IN	denotes	with
T5549	20872-20873	CD	denotes	1
T5550	20873-20874	NN	denotes	%
T5552	20875-20879	NN	denotes	goat
T5551	20880-20885	NN	denotes	serum
T5554	20886-20889	NN	denotes	PBS
T5553	20890-20898	NN	denotes	solution
T5555	20898-20900	,	denotes	,
T5556	20900-20903	DT	denotes	the
T5557	20904-20909	NNS	denotes	cells
T5558	20910-20914	VBD	denotes	were
T5545	20915-20924	VBN	denotes	incubated
T5559	20925-20929	IN	denotes	with
T5560	20930-20932	DT	denotes	an
T5562	20933-20937	NNP	denotes	Alex
T5563	20938-20941	CD	denotes	488
T5564	20942-20952	JJ	denotes	conjugated
T5565	20953-20962	JJ	denotes	secondary
T5561	20963-20971	NN	denotes	antibody
T5566	20972-20973	-LRB-	denotes	(
T5568	20973-20974	CD	denotes	1
T5569	20974-20975	SYM	denotes	:
T5570	20975-20978	CD	denotes	100
T5571	20979-20981	IN	denotes	in
T5572	20982-20983	CD	denotes	1
T5573	20983-20984	NN	denotes	%
T5575	20985-20989	NN	denotes	goat
T5574	20990-20995	NN	denotes	serum
T5577	20996-20999	NN	denotes	PBS
T5576	21000-21008	NN	denotes	solution
T5578	21008-21010	,	denotes	,
T5579	21010-21019	NNP	denotes	Molecular
T5567	21020-21026	NNP	denotes	Probes
T5580	21026-21027	-RRB-	denotes	)
T5581	21028-21031	IN	denotes	for
T5582	21032-21033	CD	denotes	3
T5583	21034-21037	NNS	denotes	hrs
T5584	21038-21040	IN	denotes	at
T5585	21041-21045	NN	denotes	room
T5586	21046-21057	NN	denotes	temperature
T5587	21057-21058	.	denotes	.
T5588	21058-21217	sentence	denotes	Following final washes with 1% goat serum PBS solution, the neuronal cells on the coverslips were cover-slipped with a glycerol-based anti-photobleach medium.
T5589	21059-21068	IN	denotes	Following
T5591	21069-21074	JJ	denotes	final
T5592	21075-21081	NNS	denotes	washes
T5593	21082-21086	IN	denotes	with
T5594	21087-21088	CD	denotes	1
T5595	21088-21089	NN	denotes	%
T5597	21090-21094	NN	denotes	goat
T5596	21095-21100	NN	denotes	serum
T5599	21101-21104	NN	denotes	PBS
T5598	21105-21113	NN	denotes	solution
T5600	21113-21115	,	denotes	,
T5601	21115-21118	DT	denotes	the
T5603	21119-21127	JJ	denotes	neuronal
T5602	21128-21133	NNS	denotes	cells
T5604	21134-21136	IN	denotes	on
T5605	21137-21140	DT	denotes	the
T5606	21141-21151	NNS	denotes	coverslips
T5607	21152-21156	VBD	denotes	were
T5608	21157-21162	NN	denotes	cover
T5609	21162-21163	HYPH	denotes	-
T5590	21163-21170	VBN	denotes	slipped
T5610	21171-21175	IN	denotes	with
T5611	21176-21177	DT	denotes	a
T5613	21178-21186	NN	denotes	glycerol
T5615	21186-21187	HYPH	denotes	-
T5614	21187-21192	VBN	denotes	based
T5616	21193-21209	JJ	denotes	anti-photobleach
T5612	21210-21216	NN	denotes	medium
T5617	21216-21217	.	denotes	.
T5618	21217-21281	sentence	denotes	The cells were viewed under a confocal microscope (Carl Zeiss).
T5619	21218-21221	DT	denotes	The
T5620	21222-21227	NNS	denotes	cells
T5622	21228-21232	VBD	denotes	were
T5621	21233-21239	VBN	denotes	viewed
T5623	21240-21245	IN	denotes	under
T5624	21246-21247	DT	denotes	a
T5626	21248-21256	JJ	denotes	confocal
T5625	21257-21267	NN	denotes	microscope
T5627	21268-21269	-LRB-	denotes	(
T5629	21269-21273	NNP	denotes	Carl
T5628	21274-21279	NNP	denotes	Zeiss
T5630	21279-21280	-RRB-	denotes	)
T5631	21280-21281	.	denotes	.
T5632	21281-21397	sentence	denotes	Images were captured with a CCD camera and acquired by the Scion Image software (Scion Corporation, Frederick, MD).
T5633	21282-21288	NNS	denotes	Images
T5635	21289-21293	VBD	denotes	were
T5634	21294-21302	VBN	denotes	captured
T5636	21303-21307	IN	denotes	with
T5637	21308-21309	DT	denotes	a
T5639	21310-21313	NN	denotes	CCD
T5638	21314-21320	NN	denotes	camera
T5640	21321-21324	CC	denotes	and
T5641	21325-21333	VBN	denotes	acquired
T5642	21334-21336	IN	denotes	by
T5643	21337-21340	DT	denotes	the
T5645	21341-21346	NNP	denotes	Scion
T5646	21347-21352	NN	denotes	Image
T5644	21353-21361	NN	denotes	software
T5647	21362-21363	-LRB-	denotes	(
T5649	21363-21368	NNP	denotes	Scion
T5648	21369-21380	NNP	denotes	Corporation
T5650	21380-21382	,	denotes	,
T5651	21382-21391	NNP	denotes	Frederick
T5652	21391-21393	,	denotes	,
T5653	21393-21395	NNP	denotes	MD
T5654	21395-21396	-RRB-	denotes	)
T5655	21396-21397	.	denotes	.
T5677	21399-21403	NN	denotes	Gene
T5678	21404-21408	NN	denotes	bank
T5680	21409-21418	NN	denotes	accession
T5679	21419-21425	NN	denotes	number
T5681	21425-21608	sentence	denotes	The cDNA sequences for the Acdp gene family have already been deposited in gene bank with accession numbers AF202994 (Acdp1), AF216961 (Acdp2), AF216964 (Acdp3) and AF216963 (Acdp4).
T5682	21426-21429	DT	denotes	The
T5684	21430-21434	NN	denotes	cDNA
T5683	21435-21444	NNS	denotes	sequences
T5686	21445-21448	IN	denotes	for
T5687	21449-21452	DT	denotes	the
T5689	21453-21457	NN	denotes	Acdp
T5690	21458-21462	NN	denotes	gene
T5688	21463-21469	NN	denotes	family
T5691	21470-21474	VBP	denotes	have
T5692	21475-21482	RB	denotes	already
T5693	21483-21487	VBN	denotes	been
T5685	21488-21497	VBN	denotes	deposited
T5694	21498-21500	IN	denotes	in
T5695	21501-21505	NN	denotes	gene
T5696	21506-21510	NN	denotes	bank
T5697	21511-21515	IN	denotes	with
T5698	21516-21525	NN	denotes	accession
T5700	21526-21533	NNS	denotes	numbers
T5699	21534-21542	NN	denotes	AF202994
T5701	21543-21544	-LRB-	denotes	(
T5702	21544-21549	NN	denotes	Acdp1
T5703	21549-21550	-RRB-	denotes	)
T5704	21550-21552	,	denotes	,
T5705	21552-21560	NN	denotes	AF216961
T5706	21561-21562	-LRB-	denotes	(
T5707	21562-21567	NN	denotes	Acdp2
T5708	21567-21568	-RRB-	denotes	)
T5709	21568-21570	,	denotes	,
T5710	21570-21578	NN	denotes	AF216964
T5711	21579-21580	-LRB-	denotes	(
T5712	21580-21585	NN	denotes	Acdp3
T5713	21585-21586	-RRB-	denotes	)
T5714	21587-21590	CC	denotes	and
T5715	21591-21599	NN	denotes	AF216963
T5716	21600-21601	-LRB-	denotes	(
T5717	21601-21606	NN	denotes	Acdp4
T5718	21606-21607	-RRB-	denotes	)
T5719	21607-21608	.	denotes	.
R1000	T2035	T2036	nmod	nucleotide,sequences
R1001	T2036	T2033	pobj	sequences,of
R1002	T2037	T2035	cc	and,nucleotide
R1003	T2038	T2035	conj	AA,nucleotide
R1004	T2039	T2032	prep	to,homologies
R1005	T2040	T2041	det	the,genes
R1006	T2041	T2039	pobj	genes,to
R1007	T2042	T2041	amod	human,genes
R1008	T2043	T2041	compound	ACDP,genes
R1009	T2044	T2045	punct	(,Table
R1010	T2045	T2029	parataxis	Table,showed
R1011	T2046	T2045	nummod	1,Table
R1012	T2047	T2045	punct	),Table
R1013	T2048	T2029	punct	.,showed
R1014	T2050	T2051	det	The,homologies
R1015	T2051	T2053	nsubjpass	homologies,observed
R1016	T2052	T2051	amod	highest,homologies
R1017	T2054	T2053	auxpass	were,observed
R1018	T2055	T2053	prep	between,observed
R1019	T2056	T2057	det	the,ACDP2
R1020	T2057	T2055	pobj	ACDP2,between
R1021	T2058	T2057	amod	human,ACDP2
R1022	T2059	T2057	cc	and,ACDP2
R1023	T2060	T2061	det	the,Acdp2
R1024	T2061	T2063	compound	Acdp2,gene
R1025	T2062	T2061	compound	mouse,Acdp2
R1026	T2063	T2057	conj	gene,ACDP2
R1027	T2064	T2065	punct	(,%
R1028	T2065	T2053	parataxis	%,observed
R1029	T2066	T2065	nummod	91,%
R1030	T2067	T2065	prep	of,%
R1031	T2068	T2069	compound	nucleotide,identity
R1032	T2069	T2067	pobj	identity,of
R1033	T2070	T2065	punct	", ",%
R1034	T2071	T2072	nummod	97,%
R1035	T2072	T2065	conj	%,%
R1036	T2073	T2072	prep	of,%
R1037	T2074	T2075	compound	AA,identity
R1038	T2075	T2073	pobj	identity,of
R1039	T2076	T2072	cc	and,%
R1040	T2077	T2078	nummod	99.4,%
R1041	T2078	T2072	conj	%,%
R1042	T2079	T2078	prep	of,%
R1043	T2080	T2081	compound	AA,homology
R1044	T2081	T2079	pobj	homology,of
R1045	T2082	T2065	punct	),%
R1046	T2083	T2053	punct	.,observed
R1047	T2085	T2086	prep	In,showed
R1048	T2086	T2105	ccomp	showed,showed
R1049	T2087	T2085	pobj	addition,In
R1050	T2088	T2086	punct	", ",showed
R1051	T2089	T2090	det	the,sequences
R1052	T2090	T2086	nsubj	sequences,showed
R1053	T2091	T2092	nummod	5,UTR
R1054	T2092	T2090	compound	UTR,sequences
R1055	T2093	T2091	punct	',5
R1056	T2094	T2090	compound	nucleotide,sequences
R1057	T2095	T2096	punct	(,bp
R1058	T2096	T2090	parataxis	bp,sequences
R1059	T2097	T2096	nummod	20,bp
R1060	T2098	T2096	prep	of,bp
R1061	T2099	T2098	pobj	nucleotides,of
R1062	T2100	T2096	prep	before,bp
R1063	T2101	T2102	compound	start,codon
R1064	T2102	T2100	pobj	codon,before
R1065	T2103	T2096	punct	),bp
R1066	T2104	T2086	advmod	also,showed
R1067	T2106	T2107	amod	high,homologies
R1068	T2107	T2086	dobj	homologies,showed
R1069	T2108	T2107	prep	to,homologies
R1070	T2109	T2110	det	the,homologs
R1071	T2110	T2108	pobj	homologs,to
R1072	T2111	T2110	amod	human,homologs
R1073	T2112	T2105	punct	", ",showed
R1074	T2113	T2105	prep	for,showed
R1075	T2114	T2113	pobj	example,for
R1076	T2115	T2105	punct	", ",showed
R1077	T2116	T2117	det	the,sequence
R1078	T2117	T2105	nsubj	sequence,showed
R1079	T2118	T2117	nmod	Acdp2,sequence
R1080	T2119	T2120	nummod	5,UTR
R1081	T2120	T2117	compound	UTR,sequence
R1082	T2121	T2119	punct	',5
R1083	T2122	T2123	nummod	95,%
R1084	T2123	T2124	compound	%,identities
R1085	T2124	T2105	dobj	identities,showed
R1086	T2125	T2105	prep	to,showed
R1087	T2126	T2127	poss	its,homolog
R1088	T2127	T2125	pobj	homolog,to
R1089	T2128	T2127	amod	human,homolog
R1090	T2129	T2105	punct	.,showed
R1091	T2131	T2132	advmod	However,were
R1092	T2133	T2132	punct	", ",were
R1093	T2134	T2135	det	the,homologies
R1094	T2135	T2132	nsubj	homologies,were
R1095	T2136	T2135	prep	in,homologies
R1096	T2137	T2138	det	the,sequences
R1097	T2138	T2136	pobj	sequences,in
R1098	T2139	T2140	nummod	3,UTR
R1099	T2140	T2138	compound	UTR,sequences
R1100	T2141	T2139	punct	',3
R1101	T2142	T2143	punct	(,bp
R1102	T2143	T2135	parataxis	bp,homologies
R1103	T2144	T2143	nummod	20,bp
R1104	T2145	T2143	prep	of,bp
R1105	T2146	T2145	pobj	nucleotides,of
R1106	T2147	T2143	prep	after,bp
R1107	T2148	T2149	compound	stop,codon
R1108	T2149	T2147	pobj	codon,after
R1109	T2150	T2143	punct	),bp
R1110	T2151	T2152	advmod	much,lower
R1111	T2152	T2132	acomp	lower,were
R1112	T2153	T2154	punct	(,%
R1113	T2154	T2132	parataxis	%,were
R1114	T2155	T2156	quantmod	40,55
R1115	T2156	T2154	nummod	55,%
R1116	T2157	T2156	punct	–,55
R1117	T2158	T2154	punct	),%
R1118	T2159	T2132	prep	for,were
R1119	T2160	T2161	det	all,genes
R1120	T2161	T2159	pobj	genes,for
R1121	T2162	T2161	compound	Acdp,genes
R1122	T2163	T2161	prep	except,genes
R1123	T2164	T2163	pobj	Acdp4,except
R1124	T2165	T2166	punct	(,identity
R1125	T2166	T2164	parataxis	identity,Acdp4
R1126	T2167	T2166	nummod	90,identity
R1127	T2168	T2167	quantmod	%,90
R1128	T2169	T2166	prep	to,identity
R1129	T2170	T2171	poss	its,homolog
R1130	T2171	T2169	pobj	homolog,to
R1131	T2172	T2171	amod	human,homolog
R1132	T2173	T2166	punct	),identity
R1133	T2174	T2132	punct	.,were
R1134	T2176	T2177	det	The,domain
R1135	T2177	T2180	nsubj	domain,has
R1136	T2178	T2177	amod	ancient,domain
R1137	T2179	T2177	amod	conserved,domain
R1138	T2181	T2177	punct	(,domain
R1139	T2182	T2177	appos	ACD,domain
R1140	T2183	T2180	punct	),has
R1141	T2184	T2185	nummod	55.3,%
R1142	T2185	T2180	dobj	%,has
R1143	T2186	T2185	prep	of,%
R1144	T2187	T2188	compound	AA,identity
R1145	T2188	T2186	pobj	identity,of
R1146	T2189	T2185	cc	and,%
R1147	T2190	T2191	nummod	83.3,%
R1148	T2191	T2185	conj	%,%
R1149	T2192	T2191	prep	of,%
R1150	T2193	T2192	pobj	homology,of
R1151	T2194	T2180	prep	between,has
R1152	T2195	T2196	det	all,proteins
R1153	T2196	T2194	pobj	proteins,between
R1154	T2197	T2196	nmod	mouse,proteins
R1155	T2198	T2197	cc	and,mouse
R1156	T2199	T2197	conj	human,mouse
R1157	T2200	T2196	compound	ACDP,proteins
R1158	T2201	T2202	punct	(,Fig.
R1159	T2202	T2180	parataxis	Fig.,has
R1160	T2203	T2202	nummod	2,Fig.
R1161	T2204	T2202	punct	),Fig.
R1162	T2205	T2180	punct	.,has
R1163	T2207	T2208	det	The,domain
R1164	T2208	T2210	nsubjpass	domain,conserved
R1165	T2209	T2208	compound	ACD,domain
R1166	T2211	T2210	auxpass	is,conserved
R1167	T2212	T2210	advmod	evolutionarily,conserved
R1168	T2213	T2210	prep	in,conserved
R1169	T2214	T2215	amod	divergent,species
R1170	T2215	T2213	pobj	species,in
R1171	T2216	T2215	acl	ranging,species
R1172	T2217	T2216	prep	from,ranging
R1173	T2218	T2217	pobj	bacteria,from
R1174	T2219	T2218	punct	", ",bacteria
R1175	T2220	T2218	appos	yeast,bacteria
R1176	T2221	T2218	punct	", ",bacteria
R1177	T2222	T2223	compound	C.,elegans
R1178	T2223	T2218	appos	elegans,bacteria
R1179	T2224	T2218	punct	", ",bacteria
R1180	T2225	T2226	compound	D.,melanogaster
R1181	T2226	T2218	appos	melanogaster,bacteria
R1182	T2227	T2218	punct	", ",bacteria
R1183	T2228	T2218	appos	mouse,bacteria
R1184	T2229	T2217	prep	to,from
R1185	T2230	T2229	pobj	human,to
R1186	T2231	T2232	punct	(,Fig.
R1187	T2232	T2210	parataxis	Fig.,conserved
R1188	T2233	T2232	nummod	3,Fig.
R1189	T2234	T2232	punct	),Fig.
R1190	T2235	T2210	punct	.,conserved
R1191	T2237	T2238	advmod	Particularly,showed
R1192	T2239	T2238	punct	", ",showed
R1193	T2240	T2241	mark	as,shown
R1194	T2241	T2238	advcl	shown,showed
R1195	T2242	T2241	prep	in,shown
R1196	T2243	T2242	pobj	Fig.,in
R1197	T2244	T2243	nummod	3,Fig.
R1198	T2245	T2238	punct	", ",showed
R1199	T2246	T2247	compound	Acdp,proteins
R1200	T2247	T2238	nsubj	proteins,showed
R1201	T2248	T2249	advmod	very,strong
R1202	T2249	T2250	amod	strong,homology
R1203	T2250	T2238	dobj	homology,showed
R1204	T2251	T2250	compound	AA,homology
R1205	T2252	T2250	prep	to,homology
R1206	T2253	T2254	compound	bacteria,protein
R1207	T2254	T2252	pobj	protein,to
R1208	T2255	T2254	compound	CorC,protein
R1209	T2256	T2257	punct	(,identity
R1210	T2257	T2254	parataxis	identity,protein
R1211	T2258	T2259	nummod	35,%
R1212	T2259	T2257	compound	%,identity
R1213	T2260	T2257	compound	AA,identity
R1214	T2261	T2257	prep	with,identity
R1215	T2262	T2263	nummod	55,%
R1216	T2263	T2264	compound	%,homology
R1217	T2264	T2261	pobj	homology,with
R1218	T2265	T2257	punct	),identity
R1219	T2266	T2254	punct	", ",protein
R1220	T2267	T2268	dep	which,involved
R1221	T2268	T2254	relcl	involved,protein
R1222	T2269	T2268	auxpass	is,involved
R1223	T2270	T2268	prep	in,involved
R1224	T2271	T2272	nmod	magnesium,efflux
R1225	T2272	T2270	pobj	efflux,in
R1226	T2273	T2271	cc	and,magnesium
R1227	T2274	T2271	conj	cobalt,magnesium
R1228	T2275	T2276	punct	[,7
R1229	T2276	T2238	parataxis	7,showed
R1230	T2277	T2276	punct	],7
R1231	T2278	T2238	punct	.,showed
R1232	T2280	T2281	amod	High,homology
R1233	T2281	T2283	nsubjpass	homology,observed
R1234	T2282	T2281	compound	AA,homology
R1235	T2284	T2283	auxpass	was,observed
R1236	T2285	T2283	advmod	also,observed
R1237	T2286	T2283	prep	between,observed
R1238	T2287	T2288	det	the,proteins
R1239	T2288	T2286	pobj	proteins,between
R1240	T2289	T2288	compound	Acdp,proteins
R1241	T2290	T2288	cc	and,proteins
R1242	T2291	T2292	det	the,protein
R1243	T2292	T2288	conj	protein,proteins
R1244	T2293	T2292	compound	yeast,protein
R1245	T2294	T2292	compound	Amip3,protein
R1246	T2295	T2296	punct	(,identity
R1247	T2296	T2283	parataxis	identity,observed
R1248	T2297	T2296	nummod	35,identity
R1249	T2298	T2297	quantmod	%,35
R1250	T2299	T2296	compound	AA,identity
R1251	T2300	T2296	prep	with,identity
R1252	T2301	T2302	nummod	56,homology
R1253	T2302	T2300	pobj	homology,with
R1254	T2303	T2301	quantmod	%,56
R1255	T2304	T2296	punct	),identity
R1256	T2305	T2283	punct	.,observed
R1257	T2307	T2308	det	The,Amip3
R1258	T2308	T2309	nsubj	Amip3,is
R1259	T2310	T2309	acomp	likely,is
R1260	T2311	T2312	aux	to,be
R1261	T2312	T2310	xcomp	be,likely
R1262	T2313	T2314	det	a,homologous
R1263	T2314	T2312	attr	homologous,be
R1264	T2315	T2314	prep	to,homologous
R1265	T2316	T2317	det	the,protein
R1266	T2317	T2315	pobj	protein,to
R1267	T2318	T2317	compound	bacteria,protein
R1268	T2319	T2317	compound	CorC,protein
R1269	T2320	T2309	punct	.,is
R1270	T2322	T2323	det	The,mutants
R1271	T2323	T2325	nsubj	mutants,confer
R1272	T2324	T2323	compound	Amip3,mutants
R1273	T2326	T2325	dobj	resistance,confer
R1274	T2327	T2326	prep	to,resistance
R1275	T2328	T2329	compound	copper,toxicity
R1276	T2329	T2327	pobj	toxicity,to
R1277	T2330	T2331	punct	(,Dr.
R1278	T2331	T2325	meta	Dr.,confer
R1279	T2332	T2331	amod	Personal,Dr.
R1280	T2333	T2331	nmod	communication,Dr.
R1281	T2334	T2331	nmod	with,Dr.
R1282	T2335	T2331	nmod	V.C.,Dr.
R1283	T2336	T2331	nmod	Culotte,Dr.
R1284	T2337	T2331	punct	", ",Dr.
R1285	T2338	T2331	nmod	John,Dr.
R1286	T2339	T2331	nmod	Hopkins,Dr.
R1287	T2340	T2331	nmod	Bloomberg,Dr.
R1288	T2341	T2331	punct	", ",Dr.
R1289	T2342	T2331	nmod	School,Dr.
R1290	T2343	T2331	nmod	of,Dr.
R1291	T2344	T2331	nmod	Public,Dr.
R1292	T2345	T2331	nmod	Health,Dr.
R1293	T2346	T2331	punct	),Dr.
R1294	T2347	T2325	punct	.,confer
R1295	T2349	T2350	det	The,relationships
R1296	T2350	T2352	nsubjpass	relationships,illustrated
R1297	T2351	T2350	amod	evolutionary,relationships
R1298	T2353	T2350	prep	among,relationships
R1299	T2354	T2355	det	those,proteins
R1300	T2355	T2353	pobj	proteins,among
R1301	T2356	T2352	auxpass	are,illustrated
R1302	T2357	T2352	prep	by,illustrated
R1303	T2358	T2359	det	a,tree
R1304	T2359	T2357	pobj	tree,by
R1305	T2360	T2359	amod	phylogenetic,tree
R1306	T2361	T2359	acl	constructed,tree
R1307	T2362	T2361	prep	based,constructed
R1308	T2363	T2362	prep	on,based
R1309	T2364	T2365	det	the,homology
R1310	T2365	T2363	pobj	homology,on
R1311	T2366	T2365	compound	AA,homology
R1312	T2367	T2365	prep	of,homology
R1313	T2368	T2367	pobj	proteins,of
R1314	T2369	T2370	punct	(,Fig.
R1315	T2370	T2352	parataxis	Fig.,illustrated
R1316	T2371	T2370	nummod	4,Fig.
R1317	T2372	T2370	punct	),Fig.
R1318	T2373	T2352	punct	.,illustrated
R1319	T2376	T2377	nsubj	We,found
R1320	T2378	T2379	mark	that,contain
R1321	T2379	T2377	ccomp	contain,found
R1322	T2380	T2381	det	all,members
R1323	T2381	T2379	nsubj	members,contain
R1324	T2382	T2381	compound	mouse,members
R1325	T2383	T2381	compound	Acdp,members
R1326	T2384	T2385	nummod	four,domains
R1327	T2385	T2379	dobj	domains,contain
R1328	T2386	T2385	amod	distinct,domains
R1329	T2387	T2385	compound	transmembrane,domains
R1330	T2388	T2389	punct	(,Fig.
R1331	T2389	T2385	parataxis	Fig.,domains
R1332	T2390	T2389	nummod	5,Fig.
R1333	T2391	T2389	punct	),Fig.
R1334	T2392	T2385	punct	", ",domains
R1335	T2393	T2394	nummod	two,domains
R1336	T2394	T2385	conj	domains,domains
R1337	T2395	T2394	compound	CBS,domains
R1338	T2396	T2394	cc	and,domains
R1339	T2397	T2398	det	a,domain
R1340	T2398	T2394	conj	domain,domains
R1341	T2399	T2398	compound	DUF21,domain
R1342	T2400	T2401	dep	that,found
R1343	T2401	T2385	relcl	found,domains
R1344	T2402	T2401	auxpass	are,found
R1345	T2403	T2401	prep	in,found
R1346	T2404	T2405	nmod	bacteria,CorC
R1347	T2405	T2406	nmod	CorC,proteins
R1348	T2406	T2403	pobj	proteins,in
R1349	T2407	T2405	cc	and,CorC
R1350	T2408	T2409	compound	yeast,Amip3
R1351	T2409	T2405	conj	Amip3,CorC
R1352	T2410	T2377	punct	.,found
R1353	T2412	T2413	compound	CBS,domains
R1354	T2413	T2414	nsubj	domains,are
R1355	T2415	T2416	amod	small,modules
R1356	T2416	T2414	attr	modules,are
R1357	T2417	T2416	amod	intracellular,modules
R1358	T2418	T2419	dep	that,found
R1359	T2419	T2416	relcl	found,modules
R1360	T2420	T2419	auxpass	are,found
R1361	T2421	T2419	advmod	mostly,found
R1362	T2422	T2419	prep	in,found
R1363	T2423	T2424	nummod	2,copies
R1364	T2424	T2422	pobj	copies,in
R1365	T2425	T2423	cc	or,2
R1366	T2426	T2423	conj	four,2
R1367	T2427	T2419	prep	within,found
R1368	T2428	T2429	det	a,protein
R1369	T2429	T2427	pobj	protein,within
R1370	T2430	T2414	punct	.,are
R1371	T2432	T2433	nsubj	Pairs,dimerise
R1372	T2434	T2432	prep	of,Pairs
R1373	T2435	T2436	compound	CBS,domains
R1374	T2436	T2434	pobj	domains,of
R1375	T2437	T2438	aux	to,form
R1376	T2438	T2433	advcl	form,dimerise
R1377	T2439	T2440	det	a,domain
R1378	T2440	T2438	dobj	domain,form
R1379	T2441	T2440	amod	stable,domain
R1380	T2442	T2440	amod	globular,domain
R1381	T2443	T2444	punct	[,8
R1382	T2444	T2433	parataxis	8,dimerise
R1383	T2445	T2444	punct	],8
R1384	T2446	T2433	punct	.,dimerise
R1385	T2448	T2449	nsubj	DUF21,is
R1386	T2450	T2451	punct	(,pfam01959.9
R1387	T2451	T2448	parataxis	pfam01959.9,DUF21
R1388	T2452	T2451	dep	CD,pfam01959.9
R1389	T2453	T2451	punct	: ,pfam01959.9
R1390	T2454	T2451	punct	),pfam01959.9
R1391	T2455	T2456	det	a,domain
R1392	T2456	T2449	attr	domain,is
R1393	T2457	T2458	advmod	newly,defined
R1394	T2458	T2456	amod	defined,domain
R1395	T2459	T2456	prep	with,domain
R1396	T2460	T2461	amod	unknown,function
R1397	T2461	T2459	pobj	function,with
R1398	T2462	T2449	punct	.,is
R1399	T2464	T2465	det	This,domain
R1400	T2465	T2466	nsubj	domain,is
R1401	T2467	T2468	det	a,region
R1402	T2468	T2466	attr	region,is
R1403	T2469	T2468	amod	transmembrane,region
R1404	T2470	T2468	cc	and,region
R1405	T2471	T2468	conj	found,region
R1406	T2472	T2473	aux	to,located
R1407	T2473	T2471	xcomp	located,found
R1408	T2474	T2473	auxpass	be,located
R1409	T2475	T2473	prep	in,located
R1410	T2476	T2477	det	the,terminus
R1411	T2477	T2475	pobj	terminus,in
R1412	T2478	T2477	compound	N,terminus
R1413	T2479	T2477	punct	-,terminus
R1414	T2480	T2477	prep	of,terminus
R1415	T2481	T2482	det	the,proteins
R1416	T2482	T2480	pobj	proteins,of
R1417	T2483	T2482	amod	adjacent,proteins
R1418	T2484	T2483	prep	to,adjacent
R1419	T2485	T2486	nummod	two,domains
R1420	T2486	T2484	pobj	domains,to
R1421	T2487	T2486	amod	intracellular,domains
R1422	T2488	T2486	compound	CBS,domains
R1423	T2489	T2466	punct	.,is
R1424	T2491	T2492	det	A,domain
R1425	T2492	T2496	nsubjpass	domain,found
R1426	T2493	T2494	npadvmod	cNMP,binding
R1427	T2494	T2492	amod	binding,domain
R1428	T2495	T2494	punct	-,binding
R1429	T2497	T2498	punct	(,domain
R1430	T2498	T2492	parataxis	domain,domain
R1431	T2499	T2500	amod	cyclic,monophosphate
R1432	T2500	T2498	nmod	monophosphate,domain
R1433	T2501	T2500	nmod	nucleotide,monophosphate
R1434	T2502	T2500	punct	-,monophosphate
R1435	T2503	T2500	punct	-,monophosphate
R1436	T2504	T2500	amod	binding,monophosphate
R1437	T2505	T2498	punct	),domain
R1438	T2506	T2496	auxpass	was,found
R1439	T2507	T2496	prep	in,found
R1440	T2508	T2509	det	all,members
R1441	T2509	T2507	pobj	members,in
R1442	T2510	T2509	compound	Acdp,members
R1443	T2511	T2496	punct	.,found
R1444	T2513	T2514	prep	In,contains
R1445	T2515	T2513	pobj	addition,In
R1446	T2516	T2514	punct	", ",contains
R1447	T2517	T2514	nsubj	Acdp1,contains
R1448	T2518	T2519	det	an,region
R1449	T2519	T2514	dobj	region,contains
R1450	T2520	T2521	npadvmod	Alanine,rich
R1451	T2521	T2519	amod	rich,region
R1452	T2522	T2521	punct	-,rich
R1453	T2523	T2524	punct	(,AAAAAAAAA
R1454	T2524	T2519	parataxis	AAAAAAAAA,region
R1455	T2525	T2524	dep	2,AAAAAAAAA
R1456	T2526	T2527	punct	–,10
R1457	T2527	T2525	prep	10,2
R1458	T2528	T2524	punct	: ,AAAAAAAAA
R1459	T2529	T2524	punct	),AAAAAAAAA
R1460	T2530	T2519	punct	", ",region
R1461	T2531	T2532	det	a,region
R1462	T2532	T2519	conj	region,region
R1463	T2533	T2534	npadvmod	Leucine,rich
R1464	T2534	T2532	amod	rich,region
R1465	T2535	T2534	punct	-,rich
R1466	T2536	T2537	punct	(,SGLRLSLLSLDPVELRVL
R1467	T2537	T2532	parataxis	SGLRLSLLSLDPVELRVL,region
R1468	T2538	T2537	dep	204,SGLRLSLLSLDPVELRVL
R1469	T2539	T2540	punct	–,257
R1470	T2540	T2538	prep	257,204
R1471	T2541	T2537	punct	: ,SGLRLSLLSLDPVELRVL
R1472	T2542	T2537	compound	LLRVRPRLYGPGGDLLPPAWLRALGALLLLALSALF,SGLRLSLLSLDPVELRVL
R1473	T2543	T2537	punct	),SGLRLSLLSLDPVELRVL
R1474	T2544	T2532	punct	", ",region
R1475	T2545	T2546	det	a,region
R1476	T2546	T2532	conj	region,region
R1477	T2547	T2548	npadvmod	Proline,rich
R1478	T2548	T2546	amod	rich,region
R1479	T2549	T2548	punct	-,rich
R1480	T2550	T2551	punct	(,PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1481	T2551	T2546	parataxis	PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP,region
R1482	T2552	T2551	dep	78,PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1483	T2553	T2554	punct	–,130
R1484	T2554	T2552	prep	130,78
R1485	T2555	T2551	punct	: ,PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1486	T2556	T2551	compound	PGPPVPAAPVPAPSLA,PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1487	T2557	T2551	punct	),PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1488	T2558	T2546	punct	", ",region
R1489	T2559	T2546	cc	and,region
R1490	T2560	T2561	nummod	two,sites
R1491	T2561	T2546	conj	sites,region
R1492	T2562	T2561	compound	amidation,sites
R1493	T2563	T2564	punct	(,SGRK
R1494	T2564	T2561	parataxis	SGRK,sites
R1495	T2565	T2566	dep	917,MGKK
R1496	T2566	T2564	dep	MGKK,SGRK
R1497	T2567	T2568	punct	–,920
R1498	T2568	T2565	prep	920,917
R1499	T2569	T2566	punct	: ,MGKK
R1500	T2570	T2564	punct	;,SGRK
R1501	T2571	T2564	dep	926,SGRK
R1502	T2572	T2573	punct	–,929
R1503	T2573	T2571	prep	929,926
R1504	T2574	T2564	punct	: ,SGRK
R1505	T2575	T2564	punct	),SGRK
R1506	T2576	T2514	punct	.,contains
R1507	T2578	T2579	nsubj	Acdp2,has
R1508	T2580	T2581	det	a,region
R1509	T2581	T2579	dobj	region,has
R1510	T2582	T2583	npadvmod	glycine,rich
R1511	T2583	T2581	amod	rich,region
R1512	T2584	T2583	punct	-,rich
R1513	T2585	T2586	punct	(,GAGGSGSASGTVGGKGGAGVAG
R1514	T2586	T2581	parataxis	GAGGSGSASGTVGGKGGAGVAG,region
R1515	T2587	T2586	dep	201,GAGGSGSASGTVGGKGGAGVAG
R1516	T2588	T2589	punct	–,222
R1517	T2589	T2587	prep	222,201
R1518	T2590	T2586	punct	: ,GAGGSGSASGTVGGKGGAGVAG
R1519	T2591	T2586	punct	),GAGGSGSASGTVGGKGGAGVAG
R1520	T2592	T2579	punct	.,has
R1521	T2594	T2595	nsubj	Acdp3,possesses
R1522	T2596	T2597	det	a,region
R1523	T2597	T2595	dobj	region,possesses
R1524	T2598	T2597	amod	large,region
R1525	T2599	T2600	npadvmod	alanine,rich
R1526	T2600	T2597	amod	rich,region
R1527	T2601	T2600	punct	-,rich
R1528	T2602	T2603	punct	(,2
R1529	T2603	T2597	parataxis	2,region
R1530	T2604	T2605	punct	–,261
R1531	T2605	T2603	prep	261,2
R1532	T2606	T2603	punct	),2
R1533	T2607	T2597	cc	and,region
R1534	T2608	T2609	det	a,region
R1535	T2609	T2597	conj	region,region
R1536	T2610	T2609	amod	large,region
R1537	T2611	T2612	npadvmod	leucine,rich
R1538	T2612	T2609	amod	rich,region
R1539	T2613	T2612	punct	-,rich
R1540	T2614	T2615	punct	(,201
R1541	T2615	T2609	parataxis	201,region
R1542	T2616	T2617	punct	–,299
R1543	T2617	T2615	prep	299,201
R1544	T2618	T2615	punct	),201
R1545	T2619	T2595	punct	.,possesses
R1546	T2621	T2622	nsubj	Acdp4,contains
R1547	T2623	T2624	det	a,pattern
R1548	T2624	T2622	dobj	pattern,contains
R1549	T2625	T2624	compound	leucine,pattern
R1550	T2626	T2624	compound	zipper,pattern
R1551	T2627	T2628	punct	(,LVMVLLVLSGIFSGLNLGLMAL
R1552	T2628	T2624	parataxis	LVMVLLVLSGIFSGLNLGLMAL,pattern
R1553	T2629	T2628	dep	185,LVMVLLVLSGIFSGLNLGLMAL
R1554	T2630	T2631	punct	–,206
R1555	T2631	T2629	prep	206,185
R1556	T2632	T2628	punct	: ,LVMVLLVLSGIFSGLNLGLMAL
R1557	T2633	T2628	punct	),LVMVLLVLSGIFSGLNLGLMAL
R1558	T2634	T2624	cc	and,pattern
R1559	T2635	T2636	det	an,site
R1560	T2636	T2624	conj	site,pattern
R1561	T2637	T2636	compound	amidation,site
R1562	T2638	T2639	punct	(,GGRR
R1563	T2639	T2636	parataxis	GGRR,site
R1564	T2640	T2639	dep	7,GGRR
R1565	T2641	T2642	punct	–,10
R1566	T2642	T2640	prep	10,7
R1567	T2643	T2639	punct	: ,GGRR
R1568	T2644	T2639	punct	),GGRR
R1569	T2645	T2622	punct	.,contains
R1572	T2862	T2863	compound	Antibody,production
R1573	T2864	T2863	punct	", ",production
R1574	T2865	T2866	compound	Western,results
R1575	T2866	T2863	conj	results,production
R1576	T2867	T2866	cc	and,results
R1577	T2868	T2869	amod	subcellular,localization
R1578	T2869	T2866	conj	localization,results
R1579	T2871	T2872	nsubjpass	Peptides,synthesized
R1580	T2873	T2871	prep	from,Peptides
R1581	T2874	T2875	nmod	Acdp1,terminuses
R1582	T2875	T2873	pobj	terminuses,from
R1583	T2876	T2875	nmod	N,terminuses
R1584	T2877	T2876	punct	-,N
R1585	T2878	T2876	punct	(,N
R1586	T2879	T2876	appos	TSFLLRVYFQPGPPATAAPVPSPT,N
R1587	T2880	T2876	punct	),N
R1588	T2881	T2876	cc	and,N
R1589	T2882	T2876	conj	C,N
R1590	T2883	T2882	punct	-,C
R1591	T2884	T2882	punct	(,C
R1592	T2885	T2882	appos	TQQLTLSPAAVPTR,C
R1593	T2886	T2875	punct	),terminuses
R1594	T2887	T2871	punct	", ",Peptides
R1595	T2888	T2889	amod	conserved,peptide
R1596	T2889	T2871	appos	peptide,Peptides
R1597	T2890	T2889	prep	from,peptide
R1598	T2891	T2892	compound	ACD,domain
R1599	T2892	T2890	pobj	domain,from
R1600	T2893	T2892	prep	of,domain
R1601	T2894	T2893	pobj	Acdp1,of
R1602	T2895	T2894	punct	(,Acdp1
R1603	T2896	T2894	appos	HNIVDILFVKDLAFVDPDDCTPLLTVTRF,Acdp1
R1604	T2897	T2872	punct	),synthesized
R1605	T2898	T2872	auxpass	were,synthesized
R1606	T2899	T2872	advmod	commercially,synthesized
R1607	T2900	T2901	punct	(,Genosys
R1608	T2901	T2872	parataxis	Genosys,synthesized
R1609	T2902	T2901	compound	Sigma,Genosys
R1610	T2903	T2901	punct	),Genosys
R1611	T2904	T2872	punct	.,synthesized
R1612	T2906	T2907	det	These,sites
R1613	T2907	T2909	nsubjpass	sites,predicted
R1614	T2908	T2907	amod	antigenic,sites
R1615	T2910	T2909	auxpass	were,predicted
R1616	T2911	T2909	prep	by,predicted
R1617	T2912	T2911	pobj	software,by
R1618	T2913	T2912	prep	from,software
R1619	T2914	T2915	compound	Sigma,Genosys
R1620	T2915	T2913	pobj	Genosys,from
R1621	T2916	T2909	cc	and,predicted
R1622	T2917	T2918	amod	polyclonal,antibodies
R1623	T2918	T2919	nsubjpass	antibodies,produced
R1624	T2919	T2909	conj	produced,predicted
R1625	T2920	T2918	prep	for,antibodies
R1626	T2921	T2922	det	each,peptide
R1627	T2922	T2920	pobj	peptide,for
R1628	T2923	T2919	auxpass	were,produced
R1629	T2924	T2919	prep	by,produced
R1630	T2925	T2924	pcomp	immunizing,by
R1631	T2926	T2925	dobj	rabbits,immunizing
R1632	T2927	T2919	punct	.,produced
R1633	T2929	T2930	aux	To,test
R1634	T2930	T2931	advcl	test,conducted
R1635	T2932	T2933	det	the,specificity
R1636	T2933	T2930	dobj	specificity,test
R1637	T2934	T2933	prep	of,specificity
R1638	T2935	T2936	det	the,antibodies
R1639	T2936	T2934	pobj	antibodies,of
R1640	T2937	T2931	punct	", ",conducted
R1641	T2938	T2931	nsubj	we,conducted
R1642	T2939	T2940	compound	Western,blot
R1643	T2940	T2942	compound	blot,analysis
R1644	T2941	T2940	punct	-,blot
R1645	T2942	T2931	dobj	analysis,conducted
R1646	T2943	T2942	prep	of,analysis
R1647	T2944	T2945	compound	mouse,brain
R1648	T2945	T2946	compound	brain,tissue
R1649	T2946	T2947	compound	tissue,extracts
R1650	T2947	T2943	pobj	extracts,of
R1651	T2948	T2931	punct	.,conducted
R1652	T2950	T2951	mark	As,shown
R1653	T2951	T2952	advcl	shown,detected
R1654	T2953	T2951	prep	in,shown
R1655	T2954	T2953	pobj	Fig.,in
R1656	T2955	T2954	nummod	6A,Fig.
R1657	T2956	T2952	punct	", ",detected
R1658	T2957	T2958	det	the,antibody
R1659	T2958	T2952	nsubj	antibody,detected
R1660	T2959	T2958	acl	produced,antibody
R1661	T2960	T2959	prep	by,produced
R1662	T2961	T2962	nmod	C,peptide
R1663	T2962	T2960	pobj	peptide,by
R1664	T2963	T2961	punct	-,C
R1665	T2964	T2961	amod	terminal,C
R1666	T2965	T2952	advmod	specifically,detected
R1667	T2966	T2952	dobj	Acdp1,detected
R1668	T2967	T2968	punct	(,lane
R1669	T2968	T2952	parataxis	lane,detected
R1670	T2969	T2968	nummod	3,lane
R1671	T2970	T2968	punct	),lane
R1672	T2971	T2952	punct	.,detected
R1673	T2973	T2974	det	The,antibody
R1674	T2974	T2975	nsubj	antibody,recognized
R1675	T2976	T2974	acl	generated,antibody
R1676	T2977	T2976	agent	by,generated
R1677	T2978	T2979	nmod	N,peptide
R1678	T2979	T2977	pobj	peptide,by
R1679	T2980	T2978	punct	-,N
R1680	T2981	T2978	amod	terminal,N
R1681	T2982	T2975	dobj	Acdp4,recognized
R1682	T2983	T2975	prep	in,recognized
R1683	T2984	T2983	pobj	addition,in
R1684	T2985	T2984	prep	to,addition
R1685	T2986	T2985	pobj	Acdp1,to
R1686	T2987	T2975	punct	", ",recognized
R1687	T2988	T2989	mark	although,was
R1688	T2989	T2975	advcl	was,recognized
R1689	T2990	T2991	det	the,reactivity
R1690	T2991	T2989	nsubj	reactivity,was
R1691	T2992	T2991	prep	to,reactivity
R1692	T2993	T2992	pobj	latter,to
R1693	T2994	T2995	advmod	significantly,higher
R1694	T2995	T2989	acomp	higher,was
R1695	T2996	T2997	punct	(,lane
R1696	T2997	T2975	parataxis	lane,recognized
R1697	T2998	T2997	dep	Fig.,lane
R1698	T2999	T2998	nummod	6A,Fig.
R1699	T3000	T2997	punct	", ",lane
R1700	T3001	T2997	nummod	2,lane
R1701	T3002	T2997	punct	),lane
R1702	T3003	T2975	punct	.,recognized
R1703	T3005	T3006	mark	As,expected
R1704	T3006	T3007	advcl	expected,detected
R1705	T3008	T3007	punct	", ",detected
R1706	T3009	T3010	det	the,antibody
R1707	T3010	T3007	nsubj	antibody,detected
R1708	T3011	T3010	acl	produced,antibody
R1709	T3012	T3011	agent	by,produced
R1710	T3013	T3014	det	the,peptide
R1711	T3014	T3012	pobj	peptide,by
R1712	T3015	T3014	amod	conserved,peptide
R1713	T3016	T3014	compound	sequence,peptide
R1714	T3017	T3018	det	all,proteins
R1715	T3018	T3007	dobj	proteins,detected
R1716	T3019	T3018	compound	Acdp,proteins
R1717	T3020	T3021	punct	(,lane
R1718	T3021	T3007	parataxis	lane,detected
R1719	T3022	T3021	dep	Fig.,lane
R1720	T3023	T3022	nummod	6A,Fig.
R1721	T3024	T3021	punct	", ",lane
R1722	T3025	T3021	nummod	1,lane
R1723	T3026	T3021	punct	),lane
R1724	T3027	T3007	punct	.,detected
R1725	T3029	T3030	aux	To,determine
R1726	T3030	T3032	advcl	determine,analyzed
R1727	T3031	T3030	advmod	further,determine
R1728	T3033	T3034	det	the,specificity
R1729	T3034	T3030	dobj	specificity,determine
R1730	T3035	T3034	prep	of,specificity
R1731	T3036	T3037	det	the,antibody
R1732	T3037	T3035	pobj	antibody,of
R1733	T3038	T3034	prep	against,specificity
R1734	T3039	T3040	det	the,terminus
R1735	T3040	T3038	pobj	terminus,against
R1736	T3041	T3040	compound	Acdp1,terminus
R1737	T3042	T3040	compound	C,terminus
R1738	T3043	T3040	punct	-,terminus
R1739	T3044	T3032	punct	", ",analyzed
R1740	T3045	T3032	nsubj	we,analyzed
R1741	T3046	T3032	dobj	extracts,analyzed
R1742	T3047	T3046	prep	of,extracts
R1743	T3048	T3049	nmod	HEK293,cells
R1744	T3049	T3047	pobj	cells,of
R1745	T3050	T3048	punct	", ",HEK293
R1746	T3051	T3048	conj	3T3,HEK293
R1747	T3052	T3051	cc	and,3T3
R1748	T3053	T3051	conj	PC12,3T3
R1749	T3054	T3032	punct	.,analyzed
R1750	T3056	T3057	det	The,results
R1751	T3057	T3058	nsubjpass	results,shown
R1752	T3059	T3058	auxpass	are,shown
R1753	T3060	T3058	prep	in,shown
R1754	T3061	T3060	pobj	Fig.,in
R1755	T3062	T3061	nummod	6B,Fig.
R1756	T3063	T3058	punct	.,shown
R1757	T3065	T3066	advmod	Apparently,detected
R1758	T3067	T3066	punct	", ",detected
R1759	T3068	T3069	det	this,antibody
R1760	T3069	T3066	nsubj	antibody,detected
R1761	T3070	T3071	det	a,band
R1762	T3071	T3066	dobj	band,detected
R1763	T3072	T3071	amod	specific,band
R1764	T3073	T3071	prep	of,band
R1765	T3074	T3073	pobj	Acdp1,of
R1766	T3075	T3066	prep	in,detected
R1767	T3076	T3077	det	all,lysates
R1768	T3077	T3075	pobj	lysates,in
R1769	T3078	T3077	compound	cell,lysates
R1770	T3079	T3066	punct	.,detected
R1771	T3081	T3082	prep	Of,are
R1772	T3083	T3081	pobj	note,Of
R1773	T3084	T3082	punct	", ",are
R1774	T3085	T3082	dep	shown,are
R1775	T3086	T3085	prep	in,shown
R1776	T3087	T3086	pobj	Fig.,in
R1777	T3088	T3087	nummod	6B,Fig.
R1778	T3089	T3082	nsubj	signals,are
R1779	T3090	T3089	prep	of,signals
R1780	T3091	T3092	nummod	10,μg
R1781	T3092	T3093	compound	μg,extracts
R1782	T3093	T3090	pobj	extracts,of
R1783	T3094	T3093	prep	of,extracts
R1784	T3095	T3096	compound	HEK293,lysates
R1785	T3096	T3094	pobj	lysates,of
R1786	T3097	T3096	compound	cell,lysates
R1787	T3098	T3093	punct	", ",extracts
R1788	T3099	T3100	nummod	100,μg
R1789	T3100	T3101	compound	μg,extracts
R1790	T3101	T3093	appos	extracts,extracts
R1791	T3102	T3101	prep	of,extracts
R1792	T3103	T3104	nmod	3T3,lysates
R1793	T3104	T3102	pobj	lysates,of
R1794	T3105	T3103	cc	and,3T3
R1795	T3106	T3103	conj	PC12,3T3
R1796	T3107	T3104	compound	cell,lysates
R1797	T3108	T3082	punct	.,are
R1798	T3110	T3111	advmod	Thus,vary
R1799	T3112	T3111	punct	", ",vary
R1800	T3113	T3114	det	the,levels
R1801	T3114	T3111	nsubj	levels,vary
R1802	T3115	T3114	compound	expression,levels
R1803	T3116	T3114	prep	of,levels
R1804	T3117	T3116	pobj	Acdp1,of
R1805	T3118	T3114	prep	in,levels
R1806	T3119	T3120	det	these,types
R1807	T3120	T3118	pobj	types,in
R1808	T3121	T3120	compound	cell,types
R1809	T3122	T3123	det	a,lot
R1810	T3123	T3111	dobj	lot,vary
R1811	T3124	T3111	punct	", ",vary
R1812	T3125	T3111	prep	with,vary
R1813	T3126	T3127	det	the,expression
R1814	T3127	T3125	pobj	expression,with
R1815	T3128	T3127	amod	highest,expression
R1816	T3129	T3127	prep	in,expression
R1817	T3130	T3131	compound	HEK293,cells
R1818	T3131	T3129	pobj	cells,in
R1819	T3132	T3111	punct	.,vary
R1820	T3134	T3135	advmod	Nevertheless,support
R1821	T3136	T3135	punct	", ",support
R1822	T3137	T3138	det	these,results
R1823	T3138	T3135	nsubj	results,support
R1824	T3139	T3138	compound	immunoblot,results
R1825	T3140	T3141	poss	our,analysis
R1826	T3141	T3135	dobj	analysis,support
R1827	T3142	T3141	prep	of,analysis
R1828	T3143	T3144	compound	brain,extracts
R1829	T3144	T3142	pobj	extracts,of
R1830	T3145	T3144	compound	tissue,extracts
R1831	T3146	T3147	mark	that,recognizes
R1832	T3147	T3141	acl	recognizes,analysis
R1833	T3148	T3149	det	the,antibody
R1834	T3149	T3147	nsubj	antibody,recognizes
R1835	T3150	T3149	prep	against,antibody
R1836	T3151	T3152	compound	Acdp1,terminus
R1837	T3152	T3150	pobj	terminus,against
R1838	T3153	T3152	compound	C,terminus
R1839	T3154	T3152	punct	-,terminus
R1840	T3155	T3147	advmod	specifically,recognizes
R1841	T3156	T3147	dobj	Acdp,recognizes
R1842	T3157	T3156	punct	-,Acdp
R1843	T3158	T3156	nummod	1,Acdp
R1844	T3159	T3135	punct	.,support
R1845	T3161	T3162	det	The,specificity
R1846	T3162	T3163	nsubj	specificity,suggests
R1847	T3164	T3162	prep	of,specificity
R1848	T3165	T3166	det	the,antibody
R1849	T3166	T3164	pobj	antibody,of
R1850	T3167	T3166	compound	Acdp1,antibody
R1851	T3168	T3169	compound	C,terminus
R1852	T3169	T3166	compound	terminus,antibody
R1853	T3170	T3169	punct	-,terminus
R1854	T3171	T3172	det	the,possibility
R1855	T3172	T3163	dobj	possibility,suggests
R1856	T3173	T3172	prep	of,possibility
R1857	T3174	T3173	pcomp	using,of
R1858	T3175	T3174	dobj	it,using
R1859	T3176	T3177	aux	to,localize
R1860	T3177	T3174	advcl	localize,using
R1861	T3178	T3177	dobj	Acdp,localize
R1862	T3179	T3178	punct	-,Acdp
R1863	T3180	T3178	nummod	1,Acdp
R1864	T3181	T3177	prep	within,localize
R1865	T3182	T3181	pobj	cells,within
R1866	T3183	T3163	punct	.,suggests
R1867	T3185	T3186	mark	Since,revealed
R1868	T3186	T3189	advcl	revealed,examined
R1869	T3187	T3188	compound	Northern,blot
R1870	T3188	T3186	nsubj	blot,revealed
R1871	T3190	T3191	advmod	almost,exclusive
R1872	T3191	T3192	amod	exclusive,expression
R1873	T3192	T3186	dobj	expression,revealed
R1874	T3193	T3192	prep	of,expression
R1875	T3194	T3193	pobj	Acdp1,of
R1876	T3195	T3186	prep	in,revealed
R1877	T3196	T3197	det	the,brain
R1878	T3197	T3195	pobj	brain,in
R1879	T3198	T3189	punct	", ",examined
R1880	T3199	T3189	nsubj	we,examined
R1881	T3200	T3201	poss	its,localization
R1882	T3201	T3189	dobj	localization,examined
R1883	T3202	T3201	amod	subcellular,localization
R1884	T3203	T3189	prep	in,examined
R1885	T3204	T3205	compound	hippocampus,neurons
R1886	T3205	T3203	pobj	neurons,in
R1887	T3206	T3205	acl	isolated,neurons
R1888	T3207	T3206	prep	from,isolated
R1889	T3208	T3209	compound	mouse,embryos
R1890	T3209	T3207	pobj	embryos,from
R1891	T3210	T3189	punct	.,examined
R1892	T3212	T3213	det	The,neurons
R1893	T3213	T3214	nsubjpass	neurons,cultured
R1894	T3215	T3214	auxpass	were,cultured
R1895	T3216	T3214	prep	on,cultured
R1896	T3217	T3218	compound	glass,coverslips
R1897	T3218	T3216	pobj	coverslips,on
R1898	T3219	T3218	acl	coated,coverslips
R1899	T3220	T3219	prep	with,coated
R1900	T3221	T3222	det	a,monolayer
R1901	T3222	T3220	pobj	monolayer,with
R1902	T3223	T3222	amod	confluent,monolayer
R1903	T3224	T3222	prep	of,monolayer
R1904	T3225	T3226	nmod	mouse,astrocytes
R1905	T3226	T3224	pobj	astrocytes,of
R1906	T3227	T3226	amod	cortical,astrocytes
R1907	T3228	T3214	prep	in,cultured
R1908	T3229	T3228	pobj	dishes,in
R1909	T3230	T3214	punct	.,cultured
R1910	T3232	T3233	nsubj	Immunostaining,using
R1911	T3234	T3233	aux	was,using
R1912	T3235	T3236	det	the,antibody
R1913	T3236	T3233	dobj	antibody,using
R1914	T3237	T3236	nmod	Acdp1,antibody
R1915	T3238	T3239	compound	C,terminus
R1916	T3239	T3241	npadvmod	terminus,specific
R1917	T3240	T3239	punct	-,terminus
R1918	T3241	T3236	amod	specific,antibody
R1919	T3242	T3233	punct	.,using
R1920	T3244	T3245	amod	Confocal,imaging
R1921	T3245	T3246	nsubj	imaging,revealed
R1922	T3247	T3248	mark	that,localized
R1923	T3248	T3246	ccomp	localized,revealed
R1924	T3249	T3248	nsubjpass	Acdp1,localized
R1925	T3250	T3248	auxpass	is,localized
R1926	T3251	T3248	advmod	predominantly,localized
R1927	T3252	T3248	prep	on,localized
R1928	T3253	T3254	det	the,membrane
R1929	T3254	T3252	pobj	membrane,on
R1930	T3255	T3254	compound	plasma,membrane
R1931	T3256	T3246	punct	.,revealed
R1932	T3258	T3259	det	A,series
R1933	T3259	T3260	nsubj	series,showed
R1934	T3261	T3259	prep	of,series
R1935	T3262	T3261	pobj	sections,of
R1936	T3263	T3262	prep	of,sections
R1937	T3264	T3265	det	a,cell
R1938	T3265	T3263	pobj	cell,of
R1939	T3266	T3259	prep	at,series
R1940	T3267	T3268	det	the,thickness
R1941	T3268	T3266	pobj	thickness,at
R1942	T3269	T3268	prep	of,thickness
R1943	T3270	T3271	nummod	0.5,micrometer
R1944	T3271	T3269	pobj	micrometer,of
R1945	T3272	T3260	advmod	clearly,showed
R1946	T3273	T3274	compound	membrane,location
R1947	T3274	T3260	dobj	location,showed
R1948	T3275	T3274	prep	of,location
R1949	T3276	T3277	compound	Acdp1,immunoreactivity
R1950	T3277	T3275	pobj	immunoreactivity,of
R1951	T3278	T3277	punct	-,immunoreactivity
R1952	T3279	T3280	punct	(,Fig.
R1953	T3280	T3277	parataxis	Fig.,immunoreactivity
R1954	T3281	T3280	nummod	7,Fig.
R1955	T3282	T3280	punct	),Fig.
R1956	T3283	T3260	punct	", ",showed
R1957	T3284	T3285	dep	which,is
R1958	T3285	T3260	advcl	is,showed
R1959	T3286	T3285	acomp	consistent,is
R1960	T3287	T3286	prep	with,consistent
R1961	T3288	T3289	det	the,observation
R1962	T3289	T3287	pobj	observation,with
R1963	T3290	T3289	prep	of,observation
R1964	T3291	T3292	compound	transmembrane,domains
R1965	T3292	T3290	pobj	domains,of
R1966	T3293	T3289	prep	within,observation
R1967	T3294	T3295	det	the,proteins
R1968	T3295	T3293	pobj	proteins,within
R1969	T3296	T3295	compound	Acdp,proteins
R1970	T3297	T3260	punct	.,showed
R1973	T3529	T3530	mark	Although,elucidated
R1974	T3530	T3542	advcl	elucidated,suggest
R1975	T3531	T3532	det	the,functions
R1976	T3532	T3530	nsubjpass	functions,elucidated
R1977	T3533	T3532	amod	exact,functions
R1978	T3534	T3532	prep	of,functions
R1979	T3535	T3536	det	the,family
R1980	T3536	T3534	pobj	family,of
R1981	T3537	T3536	compound	ACDP,family
R1982	T3538	T3536	compound	gene,family
R1983	T3539	T3530	auxpass	are,elucidated
R1984	T3540	T3530	neg	not,elucidated
R1985	T3541	T3530	advmod	yet,elucidated
R1986	T3543	T3542	punct	", ",suggest
R1987	T3544	T3545	amod	several,lines
R1988	T3545	T3542	nsubj	lines,suggest
R1989	T3546	T3545	prep	of,lines
R1990	T3547	T3546	pobj	evidence,of
R1991	T3548	T3549	mark	that,play
R1992	T3549	T3542	ccomp	play,suggest
R1993	T3550	T3551	compound	ACDP,genes
R1994	T3551	T3549	nsubj	genes,play
R1995	T3552	T3549	aux	may,play
R1996	T3553	T3554	det	an,role
R1997	T3554	T3549	dobj	role,play
R1998	T3555	T3554	amod	important,role
R1999	T3556	T3549	prep	in,play
R2000	T3557	T3558	amod	biological,processes
R2001	T3558	T3556	pobj	processes,in
R2002	T3559	T3542	punct	.,suggest
R2003	T3561	T3562	advmod	First,conserved
R2004	T3562	T3568	ccomp	conserved,expressed
R2005	T3563	T3562	punct	", ",conserved
R2006	T3564	T3565	det	these,genes
R2007	T3565	T3562	nsubjpass	genes,conserved
R2008	T3566	T3562	auxpass	are,conserved
R2009	T3567	T3562	advmod	evolutionarily,conserved
R2010	T3569	T3562	prep	in,conserved
R2011	T3570	T3571	amod	many,species
R2012	T3571	T3569	pobj	species,in
R2013	T3572	T3573	advmod	phylogenetically,divergent
R2014	T3573	T3571	amod	divergent,species
R2015	T3574	T3568	punct	;,expressed
R2016	T3575	T3576	advmod	Second,are
R2017	T3576	T3568	ccomp	are,expressed
R2018	T3577	T3576	punct	", ",are
R2019	T3578	T3579	amod	multiple,genes
R2020	T3579	T3576	nsubj	genes,are
R2021	T3580	T3576	acomp	present,are
R2022	T3581	T3576	prep	in,are
R2023	T3582	T3583	det	a,species
R2024	T3583	T3581	pobj	species,in
R2025	T3584	T3568	punct	;,expressed
R2026	T3585	T3568	advmod	Third,expressed
R2027	T3586	T3568	punct	", ",expressed
R2028	T3587	T3588	det	these,genes
R2029	T3588	T3568	nsubjpass	genes,expressed
R2030	T3589	T3568	auxpass	are,expressed
R2031	T3590	T3568	advmod	ubiquitously,expressed
R2032	T3591	T3568	prep	throughout,expressed
R2033	T3592	T3593	nmod	development,tissues
R2034	T3593	T3591	pobj	tissues,throughout
R2035	T3594	T3592	cc	and,development
R2036	T3595	T3592	conj	adult,development
R2037	T3596	T3597	punct	(,data
R2038	T3597	T3568	meta	data,expressed
R2039	T3598	T3597	amod	unpublished,data
R2040	T3599	T3597	punct	),data
R2041	T3600	T3568	punct	.,expressed
R2042	T3602	T3603	det	The,cloning
R2043	T3603	T3604	nsubj	cloning,are
R2044	T3605	T3603	cc	and,cloning
R2045	T3606	T3603	conj	characterization,cloning
R2046	T3607	T3603	prep	of,cloning
R2047	T3608	T3609	det	the,family
R2048	T3609	T3607	pobj	family,of
R2049	T3610	T3609	compound	mouse,family
R2050	T3611	T3609	compound	Acdp,family
R2051	T3612	T3609	compound	gene,family
R2052	T3613	T3614	det	a,step
R2053	T3614	T3604	attr	step,are
R2054	T3615	T3616	advmod	very,important
R2055	T3616	T3614	amod	important,step
R2056	T3617	T3614	prep	towards,step
R2057	T3618	T3619	det	the,elucidation
R2058	T3619	T3617	pobj	elucidation,towards
R2059	T3620	T3619	prep	of,elucidation
R2060	T3621	T3622	det	the,functions
R2061	T3622	T3620	pobj	functions,of
R2062	T3623	T3622	prep	of,functions
R2063	T3624	T3625	det	this,family
R2064	T3625	T3623	pobj	family,of
R2065	T3626	T3625	amod	multigene,family
R2066	T3627	T3604	punct	.,are
R2067	T3629	T3630	compound	Sequence,analyses
R2068	T3630	T3632	nsubj	analyses,revealed
R2069	T3631	T3630	compound	homology,analyses
R2070	T3633	T3634	mark	that,shared
R2071	T3634	T3632	ccomp	shared,revealed
R2072	T3635	T3636	compound	Acdp,proteins
R2073	T3636	T3634	nsubj	proteins,shared
R2074	T3637	T3638	advmod	very,strong
R2075	T3638	T3639	amod	strong,homologies
R2076	T3639	T3634	dobj	homologies,shared
R2077	T3640	T3639	compound	AA,homologies
R2078	T3641	T3639	prep	to,homologies
R2079	T3642	T3643	det	the,protein
R2080	T3643	T3641	pobj	protein,to
R2081	T3644	T3643	compound	bacteria,protein
R2082	T3645	T3643	compound	CorC,protein
R2083	T3646	T3643	cc	and,protein
R2084	T3647	T3648	compound	yeast,protein
R2085	T3648	T3643	conj	protein,protein
R2086	T3649	T3648	compound	Amip3,protein
R2087	T3650	T3632	punct	.,revealed
R2088	T3652	T3653	nsubj	CorA,belong
R2089	T3654	T3652	punct	", ",CorA
R2090	T3655	T3652	conj	B,CorA
R2091	T3656	T3655	punct	", ",B
R2092	T3657	T3655	conj	C,B
R2093	T3658	T3657	cc	and,C
R2094	T3659	T3657	conj	D,C
R2095	T3660	T3653	prep	to,belong
R2096	T3661	T3662	det	a,family
R2097	T3662	T3660	pobj	family,to
R2098	T3663	T3662	compound	protein,family
R2099	T3664	T3662	acl	involved,family
R2100	T3665	T3664	prep	in,involved
R2101	T3666	T3667	preconj	both,influx
R2102	T3667	T3665	pobj	influx,in
R2103	T3668	T3667	cc	and,influx
R2104	T3669	T3667	conj	efflux,influx
R2105	T3670	T3667	prep	of,influx
R2106	T3671	T3670	pobj	magnesium,of
R2107	T3672	T3671	cc	and,magnesium
R2108	T3673	T3671	conj	cobalt,magnesium
R2109	T3674	T3653	punct	.,belong
R2110	T3676	T3677	nsubjpass	It,shown
R2111	T3678	T3677	aux	has,shown
R2112	T3679	T3677	auxpass	been,shown
R2113	T3680	T3681	mark	that,confer
R2114	T3681	T3677	ccomp	confer,shown
R2115	T3682	T3683	compound	CorA,mutants
R2116	T3683	T3681	nsubj	mutants,confer
R2117	T3684	T3681	dobj	resistance,confer
R2118	T3685	T3684	prep	to,resistance
R2119	T3686	T3687	compound	cobalt,toxicity
R2120	T3687	T3685	pobj	toxicity,to
R2121	T3688	T3689	punct	[,9
R2122	T3689	T3677	parataxis	9,shown
R2123	T3690	T3689	punct	],9
R2124	T3691	T3677	punct	.,shown
R2125	T3693	T3694	nsubj	Amip3,appears
R2126	T3695	T3696	aux	to,be
R2127	T3696	T3694	xcomp	be,appears
R2128	T3697	T3698	det	a,homologous
R2129	T3698	T3696	attr	homologous,be
R2130	T3699	T3698	prep	to,homologous
R2131	T3700	T3701	compound	CorC,protein
R2132	T3701	T3699	pobj	protein,to
R2133	T3702	T3703	dep	which,involved
R2134	T3703	T3701	relcl	involved,protein
R2135	T3704	T3703	auxpass	is,involved
R2136	T3705	T3703	prep	in,involved
R2137	T3706	T3705	pobj	efflux,in
R2138	T3707	T3706	prep	of,efflux
R2139	T3708	T3707	pobj	magnesium,of
R2140	T3709	T3708	cc	and,magnesium
R2141	T3710	T3708	conj	cobalt,magnesium
R2142	T3711	T3703	prep	in,involved
R2143	T3712	T3711	pobj	bacteria,in
R2144	T3713	T3694	punct	.,appears
R2145	T3715	T3716	compound	Amip3,mutants
R2146	T3716	T3717	nsubj	mutants,confer
R2147	T3718	T3717	dobj	resistant,confer
R2148	T3719	T3718	prep	to,resistant
R2149	T3720	T3721	compound	copper,toxicity
R2150	T3721	T3719	pobj	toxicity,to
R2151	T3722	T3717	punct	.,confer
R2152	T3724	T3725	nsubj	Acdp,possess
R2153	T3726	T3725	dep	proteins,possess
R2154	T3727	T3725	advmod	also,possess
R2155	T3728	T3729	det	the,domains
R2156	T3729	T3725	dobj	domains,possess
R2157	T3730	T3731	dep	that,found
R2158	T3731	T3729	relcl	found,domains
R2159	T3732	T3731	auxpass	are,found
R2160	T3733	T3731	prep	in,found
R2161	T3734	T3735	nmod	bacteria,CorC
R2162	T3735	T3736	nmod	CorC,proteins
R2163	T3736	T3733	pobj	proteins,in
R2164	T3737	T3735	cc	and,CorC
R2165	T3738	T3739	compound	yeast,Amip3
R2166	T3739	T3735	conj	Amip3,CorC
R2167	T3740	T3729	punct	", ",domains
R2168	T3741	T3742	amod	such,as
R2169	T3742	T3729	prep	as,domains
R2170	T3743	T3744	det	the,domains
R2171	T3744	T3742	pobj	domains,as
R2172	T3745	T3744	compound	CBS,domains
R2173	T3746	T3744	punct	", ",domains
R2174	T3747	T3748	compound	DUF21,domain
R2175	T3748	T3744	conj	domain,domains
R2176	T3749	T3748	cc	and,domain
R2177	T3750	T3751	compound	transmembrane,domains
R2178	T3751	T3748	conj	domains,domain
R2179	T3752	T3725	punct	.,possess
R2180	T3754	T3755	prep	In,found
R2181	T3756	T3754	pobj	addition,In
R2182	T3757	T3755	punct	", ",found
R2183	T3758	T3759	det	a,domain
R2184	T3759	T3755	nsubjpass	domain,found
R2185	T3760	T3761	npadvmod	cNMP,binding
R2186	T3761	T3759	amod	binding,domain
R2187	T3762	T3761	punct	-,binding
R2188	T3763	T3755	auxpass	was,found
R2189	T3764	T3755	prep	in,found
R2190	T3765	T3766	det	all,proteins
R2191	T3766	T3764	pobj	proteins,in
R2192	T3767	T3766	compound	Acdp,proteins
R2193	T3768	T3755	punct	", ",found
R2194	T3769	T3770	dep	which,is
R2195	T3770	T3755	ccomp	is,found
R2196	T3771	T3770	advmod	usually,is
R2197	T3772	T3770	acomp	present,is
R2198	T3773	T3770	prep	in,is
R2199	T3774	T3775	compound	ion,channels
R2200	T3775	T3773	pobj	channels,in
R2201	T3776	T3775	cc	and,channels
R2202	T3777	T3778	nmod	cNMP,kinases
R2203	T3778	T3775	conj	kinases,channels
R2204	T3779	T3777	punct	-,cNMP
R2205	T3780	T3777	amod	dependent,cNMP
R2206	T3781	T3782	punct	[,10
R2207	T3782	T3755	parataxis	10,found
R2208	T3783	T3784	punct	-,13
R2209	T3784	T3782	prep	13,10
R2210	T3785	T3782	punct	],10
R2211	T3786	T3755	punct	.,found
R2212	T3788	T3789	advcl	Using,shown
R2213	T3790	T3788	dobj	antibody,Using
R2214	T3791	T3790	acl	produced,antibody
R2215	T3792	T3791	agent	by,produced
R2216	T3793	T3794	nmod	Acdp1,peptide
R2217	T3794	T3792	pobj	peptide,by
R2218	T3795	T3796	npadvmod	C,terminal
R2219	T3796	T3794	amod	terminal,peptide
R2220	T3797	T3796	punct	-,terminal
R2221	T3798	T3789	punct	", ",shown
R2222	T3799	T3789	nsubj	we,shown
R2223	T3800	T3789	aux	have,shown
R2224	T3801	T3802	mark	that,localized
R2225	T3802	T3789	ccomp	localized,shown
R2226	T3803	T3802	nsubjpass	Acdp1,localized
R2227	T3804	T3802	auxpass	is,localized
R2228	T3805	T3802	advmod	predominantly,localized
R2229	T3806	T3802	prep	on,localized
R2230	T3807	T3808	det	the,membrane
R2231	T3808	T3806	pobj	membrane,on
R2232	T3809	T3808	compound	plasma,membrane
R2233	T3810	T3802	prep	in,localized
R2234	T3811	T3812	compound	hippocampus,neurons
R2235	T3812	T3810	pobj	neurons,in
R2236	T3813	T3789	punct	.,shown
R2237	T3815	T3816	prep	In,found
R2238	T3817	T3818	poss	our,study
R2239	T3818	T3815	pobj	study,In
R2240	T3819	T3818	amod	previous,study
R2241	T3820	T3816	punct	", ",found
R2242	T3821	T3816	nsubj	we,found
R2243	T3822	T3823	amod	human,proteins
R2244	T3823	T3825	nsubjpass	proteins,localized
R2245	T3824	T3823	compound	ACDP,proteins
R2246	T3825	T3816	advcl	localized,found
R2247	T3826	T3825	auxpass	are,localized
R2248	T3827	T3825	advmod	predominantly,localized
R2249	T3828	T3825	prep	in,localized
R2250	T3829	T3830	det	the,nucleus
R2251	T3830	T3828	pobj	nucleus,in
R2252	T3831	T3825	prep	in,localized
R2253	T3832	T3833	compound	HeLa,cells
R2254	T3833	T3831	pobj	cells,in
R2255	T3834	T3835	punct	[,1
R2256	T3835	T3816	parataxis	1,found
R2257	T3836	T3835	punct	],1
R2258	T3837	T3816	punct	.,found
R2259	T3839	T3840	det	The,discrepancy
R2260	T3840	T3841	nsubjpass	discrepancy,caused
R2261	T3842	T3840	prep	for,discrepancy
R2262	T3843	T3844	compound	Acdp,localization
R2263	T3844	T3842	pobj	localization,for
R2264	T3845	T3844	prep	in,localization
R2265	T3846	T3847	amod	neuronal,cells
R2266	T3847	T3845	pobj	cells,in
R2267	T3848	T3841	aux	could,caused
R2268	T3849	T3841	auxpass	be,caused
R2269	T3850	T3841	agent	by,caused
R2270	T3851	T3850	pobj	non-specificity,by
R2271	T3852	T3851	prep	for,non-specificity
R2272	T3853	T3854	amod	previous,antibody
R2273	T3854	T3852	pobj	antibody,for
R2274	T3855	T3856	dep	which,produced
R2275	T3856	T3854	relcl	produced,antibody
R2276	T3857	T3856	auxpass	was,produced
R2277	T3858	T3856	prep	by,produced
R2278	T3859	T3860	det	the,domain
R2279	T3860	T3858	pobj	domain,by
R2280	T3861	T3860	amod	recombinant,domain
R2281	T3862	T3860	compound	ACD,domain
R2282	T3863	T3851	punct	", ",non-specificity
R2283	T3864	T3851	cc	or,non-specificity
R2284	T3865	T3866	amod	different,cells
R2285	T3866	T3851	conj	cells,non-specificity
R2286	T3867	T3866	acl	used,cells
R2287	T3868	T3841	punct	.,caused
R2288	T3870	T3871	prep	In,recognizes
R2289	T3872	T3873	amod	current,study
R2290	T3873	T3870	pobj	study,In
R2291	T3874	T3871	punct	", ",recognizes
R2292	T3875	T3876	det	the,antibody
R2293	T3876	T3871	nsubj	antibody,recognizes
R2294	T3877	T3876	compound	Acdp1,antibody
R2295	T3878	T3879	compound	C,terminus
R2296	T3879	T3876	compound	terminus,antibody
R2297	T3880	T3879	punct	-,terminus
R2298	T3881	T3871	advmod	only,recognizes
R2299	T3882	T3871	dobj	Acdp1,recognizes
R2300	T3883	T3871	prep	in,recognizes
R2301	T3884	T3885	compound	brain,tissue
R2302	T3885	T3886	compound	tissue,extracts
R2303	T3886	T3883	pobj	extracts,in
R2304	T3887	T3888	advmod	as,as
R2305	T3888	T3883	cc	as,in
R2306	T3889	T3888	advmod	well,as
R2307	T3890	T3883	conj	in,in
R2308	T3891	T3892	nmod	HEK293,lysates
R2309	T3892	T3890	pobj	lysates,in
R2310	T3893	T3891	punct	", ",HEK293
R2311	T3894	T3891	conj	3T3,HEK293
R2312	T3895	T3894	cc	and,3T3
R2313	T3896	T3894	conj	PC12,3T3
R2314	T3897	T3892	compound	cell,lysates
R2315	T3898	T3871	punct	", ",recognizes
R2316	T3899	T3871	advcl	suggesting,recognizes
R2317	T3900	T3901	det	the,specificity
R2318	T3901	T3899	dobj	specificity,suggesting
R2319	T3902	T3901	prep	of,specificity
R2320	T3903	T3904	det	the,antibody
R2321	T3904	T3902	pobj	antibody,of
R2322	T3905	T3871	punct	.,recognizes
R2323	T3907	T3908	poss	Our,observations
R2324	T3908	T3909	nsubj	observations,suggested
R2325	T3910	T3911	mark	that,involved
R2326	T3911	T3909	ccomp	involved,suggested
R2327	T3912	T3911	nsubjpass	Acdp,involved
R2328	T3913	T3911	aux	might,involved
R2329	T3914	T3911	auxpass	be,involved
R2330	T3915	T3911	prep	in,involved
R2331	T3916	T3917	compound	ion,transport
R2332	T3917	T3915	pobj	transport,in
R2333	T3918	T3911	prep	in,involved
R2334	T3919	T3920	amod	mammalian,cells
R2335	T3920	T3918	pobj	cells,in
R2336	T3921	T3909	punct	.,suggested
R2337	T3923	T3924	advmod	However,needed
R2338	T3925	T3924	punct	", ",needed
R2339	T3926	T3927	advmod	more,detailed
R2340	T3927	T3928	amod	detailed,studies
R2341	T3928	T3924	nsubjpass	studies,needed
R2342	T3929	T3928	amod	functional,studies
R2343	T3930	T3924	auxpass	are,needed
R2344	T3931	T3932	aux	to,demonstrate
R2345	T3932	T3924	advcl	demonstrate,needed
R2346	T3933	T3934	det	the,functions
R2347	T3934	T3932	dobj	functions,demonstrate
R2348	T3935	T3934	amod	real,functions
R2349	T3936	T3934	prep	of,functions
R2350	T3937	T3938	det	these,proteins
R2351	T3938	T3936	pobj	proteins,of
R2352	T3939	T3924	punct	.,needed
R2353	T4051	T4052	poss	Our,work
R2354	T4052	T4054	nsubj	work,described
R2355	T4053	T4052	amod	previous,work
R2356	T4055	T4054	aux	has,described
R2357	T4056	T4057	amod	human,genes
R2358	T4057	T4054	dobj	genes,described
R2359	T4058	T4057	compound	ACDP,genes
R2360	T4059	T4060	punct	[,1
R2361	T4060	T4054	parataxis	1,described
R2362	T4061	T4060	punct	],1
R2363	T4062	T4054	punct	.,described
R2364	T4064	T4065	det	The,information
R2365	T4065	T4067	nsubj	information,includes
R2366	T4066	T4065	amod	new,information
R2367	T4067	T4072	ccomp	includes,is
R2368	T4068	T4065	prep	of,information
R2369	T4069	T4070	det	the,work
R2370	T4070	T4068	pobj	work,of
R2371	T4071	T4070	amod	current,work
R2372	T4073	T4067	punct	: ,includes
R2373	T4074	T4072	meta	1,is
R2374	T4075	T4074	punct	),1
R2375	T4076	T4074	punct	.,1
R2376	T4077	T4072	nsubj	It,is
R2377	T4078	T4079	det	the,time
R2378	T4079	T4072	attr	time,is
R2379	T4080	T4079	amod	first,time
R2380	T4081	T4082	aux	to,report
R2381	T4082	T4079	advcl	report,time
R2382	T4083	T4084	det	the,existence
R2383	T4084	T4082	dobj	existence,report
R2384	T4085	T4084	prep	of,existence
R2385	T4086	T4087	amod	multiple,genes
R2386	T4087	T4085	pobj	genes,of
R2387	T4088	T4087	compound	Acdp,genes
R2388	T4089	T4082	prep	in,report
R2389	T4090	T4091	amod	other,mammals
R2390	T4091	T4089	pobj	mammals,in
R2391	T4092	T4091	prep	in,mammals
R2392	T4093	T4092	pobj	addition,in
R2393	T4094	T4093	prep	to,addition
R2394	T4095	T4094	pobj	human,to
R2395	T4096	T4072	punct	", ",is
R2396	T4097	T4098	mark	while,appears
R2397	T4098	T4072	advcl	appears,is
R2398	T4099	T4098	nsubj	Acdp,appears
R2399	T4100	T4101	aux	to,be
R2400	T4101	T4098	xcomp	be,appears
R2401	T4102	T4103	det	a,gene
R2402	T4103	T4101	attr	gene,be
R2403	T4104	T4103	amod	single,gene
R2404	T4105	T4103	compound	copy,gene
R2405	T4106	T4103	prep	in,gene
R2406	T4107	T4108	amod	lower,organisms
R2407	T4108	T4106	pobj	organisms,in
R2408	T4109	T4110	amod	such,as
R2409	T4110	T4101	prep	as,be
R2410	T4111	T4110	prep	in,as
R2411	T4112	T4113	compound	C.,elegans
R2412	T4113	T4111	pobj	elegans,in
R2413	T4114	T4113	punct	", ",elegans
R2414	T4115	T4113	conj	yeasts,elegans
R2415	T4116	T4115	cc	and,yeasts
R2416	T4117	T4115	conj	bacteria,yeasts
R2417	T4118	T4072	punct	.,is
R2418	T4120	T4121	nsubj	We,suggested
R2419	T4121	T4124	ccomp	suggested,generated
R2420	T4122	T4121	aux	have,suggested
R2421	T4123	T4121	advmod	also,suggested
R2422	T4125	T4126	det	the,relationships
R2423	T4126	T4121	dobj	relationships,suggested
R2424	T4127	T4126	amod	evolutionary,relationships
R2425	T4128	T4126	prep	of,relationships
R2426	T4129	T4130	compound	Acdp,genes
R2427	T4130	T4128	pobj	genes,of
R2428	T4131	T4130	prep	in,genes
R2429	T4132	T4133	amod	different,species
R2430	T4133	T4131	pobj	species,in
R2431	T4134	T4135	punct	(,tree
R2432	T4135	T4121	parataxis	tree,suggested
R2433	T4136	T4135	amod	phylogenetic,tree
R2434	T4137	T4135	punct	),tree
R2435	T4138	T4124	punct	;,generated
R2436	T4139	T4140	meta	2,are
R2437	T4140	T4124	ccomp	are,generated
R2438	T4141	T4139	punct	),2
R2439	T4142	T4139	punct	.,2
R2440	T4143	T4144	amod	Molecular,cloning
R2441	T4144	T4140	nsubj	cloning,are
R2442	T4145	T4144	cc	and,cloning
R2443	T4146	T4144	conj	characterization,cloning
R2444	T4147	T4144	prep	of,cloning
R2445	T4148	T4149	amod	murine,family
R2446	T4149	T4147	pobj	family,of
R2447	T4150	T4149	compound	Acdp,family
R2448	T4151	T4149	compound	gene,family
R2449	T4152	T4140	acomp	essential,are
R2450	T4153	T4140	prep	for,are
R2451	T4154	T4153	pobj	study,for
R2452	T4155	T4154	prep	of,study
R2453	T4156	T4157	det	this,family
R2454	T4157	T4155	pobj	family,of
R2455	T4158	T4157	amod	novel,family
R2456	T4159	T4157	compound	gene,family
R2457	T4160	T4154	prep	in,study
R2458	T4161	T4162	compound	animal,model
R2459	T4162	T4160	pobj	model,in
R2460	T4163	T4153	punct	", ",for
R2461	T4164	T4165	advmod	e.g.,for
R2462	T4165	T4153	prep	for,for
R2463	T4166	T4165	pobj	generation,for
R2464	T4167	T4166	prep	of,generation
R2465	T4168	T4169	nmod	knockout,models
R2466	T4169	T4167	pobj	models,of
R2467	T4170	T4168	cc	or,knockout
R2468	T4171	T4168	conj	transgenic,knockout
R2469	T4172	T4124	punct	;,generated
R2470	T4173	T4174	meta	3,demonstrated
R2471	T4174	T4124	ccomp	demonstrated,generated
R2472	T4175	T4173	punct	),3
R2473	T4176	T4173	punct	.,3
R2474	T4177	T4174	nsubj	We,demonstrated
R2475	T4178	T4174	aux	have,demonstrated
R2476	T4179	T4180	preconj	both,DNA
R2477	T4180	T4181	nmod	DNA,conservations
R2478	T4181	T4174	dobj	conservations,demonstrated
R2479	T4182	T4180	cc	and,DNA
R2480	T4183	T4184	compound	amino,acid
R2481	T4184	T4180	conj	acid,DNA
R2482	T4185	T4181	prep	between,conservations
R2483	T4186	T4185	pobj	mouse,between
R2484	T4187	T4186	cc	and,mouse
R2485	T4188	T4186	conj	human,mouse
R2486	T4189	T4174	prep	for,demonstrated
R2487	T4190	T4191	det	each,gene
R2488	T4191	T4189	pobj	gene,for
R2489	T4192	T4191	compound	Acdp,gene
R2490	T4193	T4191	cc	and,gene
R2491	T4194	T4195	det	the,family
R2492	T4195	T4191	conj	family,gene
R2493	T4196	T4195	amod	whole,family
R2494	T4197	T4195	compound	Acdp,family
R2495	T4198	T4195	compound	gene,family
R2496	T4199	T4191	punct	", ",gene
R2497	T4200	T4201	dep	which,provide
R2498	T4201	T4191	relcl	provide,gene
R2499	T4202	T4203	amod	important,information
R2500	T4203	T4201	dobj	information,provide
R2501	T4204	T4203	prep	for,information
R2502	T4205	T4206	det	the,possibility
R2503	T4206	T4204	pobj	possibility,for
R2504	T4207	T4206	prep	of,possibility
R2505	T4208	T4209	amod	functional,redundancy
R2506	T4209	T4207	pobj	redundancy,of
R2507	T4210	T4209	cc	or,redundancy
R2508	T4211	T4209	conj	overlap,redundancy
R2509	T4212	T4209	prep	between,redundancy
R2510	T4213	T4214	compound	Acdp,members
R2511	T4214	T4212	pobj	members,between
R2512	T4215	T4124	punct	;,generated
R2513	T4216	T4124	meta	4,generated
R2514	T4217	T4216	punct	),4
R2515	T4218	T4216	punct	.,4
R2516	T4219	T4124	nsubj	We,generated
R2517	T4220	T4124	aux	have,generated
R2518	T4221	T4124	dobj	antibodies,generated
R2519	T4222	T4221	amod	specific,antibodies
R2520	T4223	T4222	prep	for,specific
R2521	T4224	T4223	pobj	Acdp1,for
R2522	T4225	T4224	cc	and,Acdp1
R2523	T4226	T4227	det	all,proteins
R2524	T4227	T4224	conj	proteins,Acdp1
R2525	T4228	T4227	compound	Acdp,proteins
R2526	T4229	T4124	punct	.,generated
R2527	T4231	T4232	det	The,antibody
R2528	T4232	T4237	nsubj	antibody,appears
R2529	T4233	T4232	compound	Acdp1,antibody
R2530	T4234	T4235	compound	C,terminus
R2531	T4235	T4232	compound	terminus,antibody
R2532	T4236	T4235	punct	-,terminus
R2533	T4238	T4239	aux	to,recognize
R2534	T4239	T4237	xcomp	recognize,appears
R2535	T4240	T4239	advmod	specifically,recognize
R2536	T4241	T4239	dobj	Acdp1,recognize
R2537	T4242	T4237	punct	.,appears
R2538	T4244	T4245	advcl	Using,demonstrated
R2539	T4246	T4247	det	this,antibody
R2540	T4247	T4244	dobj	antibody,Using
R2541	T4248	T4245	punct	", ",demonstrated
R2542	T4249	T4245	nsubj	we,demonstrated
R2543	T4250	T4245	aux	have,demonstrated
R2544	T4251	T4252	mark	that,localized
R2545	T4252	T4245	ccomp	localized,demonstrated
R2546	T4253	T4252	nsubjpass	Acdp1,localized
R2547	T4254	T4252	auxpass	is,localized
R2548	T4255	T4252	advmod	predominantly,localized
R2549	T4256	T4252	prep	on,localized
R2550	T4257	T4258	det	the,membrane
R2551	T4258	T4256	pobj	membrane,on
R2552	T4259	T4258	compound	plasma,membrane
R2553	T4260	T4252	prep	in,localized
R2554	T4261	T4262	compound	hippocampus,neurons
R2555	T4262	T4260	pobj	neurons,in
R2556	T4263	T4245	punct	.,demonstrated
R2557	T4265	T4266	det	These,results
R2558	T4266	T4267	nsubj	results,represent
R2559	T4268	T4269	det	an,step
R2560	T4269	T4267	dobj	step,represent
R2561	T4270	T4269	amod	important,step
R2562	T4271	T4269	prep	towards,step
R2563	T4272	T4273	det	the,characterization
R2564	T4273	T4271	pobj	characterization,towards
R2565	T4274	T4273	prep	of,characterization
R2566	T4275	T4274	pobj	functions,of
R2567	T4276	T4273	prep	for,characterization
R2568	T4277	T4278	det	the,family
R2569	T4278	T4276	pobj	family,for
R2570	T4279	T4278	compound	Acdp,family
R2571	T4280	T4278	compound	gene,family
R2572	T4281	T4267	punct	.,represent
R2573	T4392	T4393	compound	cDNA,cloning
R2574	T4395	T4396	compound	cDNA,cloning
R2575	T4396	T4397	nsubjpass	cloning,performed
R2576	T4398	T4396	prep	of,cloning
R2577	T4399	T4400	det	the,family
R2578	T4400	T4398	pobj	family,of
R2579	T4401	T4400	compound	Acdp,family
R2580	T4402	T4400	compound	gene,family
R2581	T4403	T4397	auxpass	was,performed
R2582	T4404	T4397	prep	based,performed
R2583	T4405	T4404	prep	on,based
R2584	T4406	T4407	amod	human,sequences
R2585	T4407	T4405	pobj	sequences,on
R2586	T4408	T4407	compound	homologue,sequences
R2587	T4409	T4410	mark	as,reported
R2588	T4410	T4397	advcl	reported,performed
R2589	T4411	T4410	advmod	previously,reported
R2590	T4412	T4413	punct	[,2
R2591	T4413	T4397	parataxis	2,performed
R2592	T4414	T4413	punct	],2
R2593	T4415	T4397	punct	.,performed
R2594	T4417	T4418	advmod	Briefly,used
R2595	T4419	T4418	punct	", ",used
R2596	T4420	T4421	det	the,cDNAs
R2597	T4421	T4418	nsubjpass	cDNAs,used
R2598	T4422	T4421	amod	human,cDNAs
R2599	T4423	T4421	compound	ACDP,cDNAs
R2600	T4424	T4421	cc	and,cDNAs
R2601	T4425	T4426	amod	predicted,sequences
R2602	T4426	T4421	conj	sequences,cDNAs
R2603	T4427	T4426	compound	AA,sequences
R2604	T4428	T4418	auxpass	were,used
R2605	T4429	T4430	aux	to,search
R2606	T4430	T4418	advcl	search,used
R2607	T4431	T4432	det	the,database
R2608	T4432	T4430	dobj	database,search
R2609	T4433	T4432	compound	mouse,database
R2610	T4434	T4432	compound	EST,database
R2611	T4435	T4430	prep	for,search
R2612	T4436	T4437	compound	EST,markers
R2613	T4437	T4435	pobj	markers,for
R2614	T4438	T4437	acl	corresponding,markers
R2615	T4439	T4438	prep	to,corresponding
R2616	T4440	T4441	det	each,member
R2617	T4441	T4439	pobj	member,to
R2618	T4442	T4441	compound	Acdp,member
R2619	T4443	T4418	punct	.,used
R2620	T4445	T4446	det	A,primer
R2621	T4446	T4448	nsubjpass	primer,used
R2622	T4447	T4446	amod	forward,primer
R2623	T4449	T4446	prep	within,primer
R2624	T4450	T4451	det	the,region
R2625	T4451	T4449	pobj	region,within
R2626	T4452	T4451	amod	human,region
R2627	T4453	T4451	nmod	ACDP,region
R2628	T4454	T4451	nummod	5,region
R2629	T4455	T4454	punct	',5
R2630	T4456	T4451	compound	cDNA,region
R2631	T4457	T4451	compound	coding,region
R2632	T4458	T4446	punct	(,primer
R2633	T4459	T4446	prep	after,primer
R2634	T4460	T4461	compound	start,codon
R2635	T4461	T4459	pobj	codon,after
R2636	T4462	T4446	punct	),primer
R2637	T4463	T4446	cc	and,primer
R2638	T4464	T4465	det	a,primer
R2639	T4465	T4446	conj	primer,primer
R2640	T4466	T4465	nmod	mouse,primer
R2641	T4467	T4465	amod	reverse,primer
R2642	T4468	T4465	prep	from,primer
R2643	T4469	T4470	det	the,marker
R2644	T4470	T4468	pobj	marker,from
R2645	T4471	T4470	compound	mouse,marker
R2646	T4472	T4470	compound	EST,marker
R2647	T4473	T4448	auxpass	were,used
R2648	T4474	T4475	aux	to,amplify
R2649	T4475	T4448	advcl	amplify,used
R2650	T4476	T4477	det	the,sequence
R2651	T4477	T4475	dobj	sequence,amplify
R2652	T4478	T4477	compound	homologue,sequence
R2653	T4479	T4475	prep	from,amplify
R2654	T4480	T4481	compound	mouse,cDNA
R2655	T4481	T4479	pobj	cDNA,from
R2656	T4482	T4475	prep	at,amplify
R2657	T4483	T4484	advmod	very,low
R2658	T4484	T4485	amod	low,temperature
R2659	T4485	T4482	pobj	temperature,at
R2660	T4486	T4485	amod	annealing,temperature
R2661	T4487	T4485	punct	(,temperature
R2662	T4488	T4489	quantmod	45,50
R2663	T4489	T4491	nummod	50,°C
R2664	T4490	T4489	punct	–,50
R2665	T4491	T4485	appos	°C,temperature
R2666	T4492	T4448	punct	),used
R2667	T4493	T4448	punct	.,used
R2668	T4495	T4496	det	A,PCR
R2669	T4496	T4498	nsubjpass	PCR,carried
R2670	T4497	T4496	amod	nested,PCR
R2671	T4499	T4496	acl	using,PCR
R2672	T4500	T4501	det	an,primer
R2673	T4501	T4499	dobj	primer,using
R2674	T4502	T4501	amod	inside,primer
R2675	T4503	T4501	amod	reverse,primer
R2676	T4504	T4501	prep	from,primer
R2677	T4505	T4506	det	the,sequence
R2678	T4506	T4504	pobj	sequence,from
R2679	T4507	T4506	compound	mouse,sequence
R2680	T4508	T4506	compound	EST,sequence
R2681	T4509	T4501	cc	and,primer
R2682	T4510	T4511	det	the,primer
R2683	T4511	T4501	conj	primer,primer
R2684	T4512	T4511	amod	same,primer
R2685	T4513	T4511	amod	human,primer
R2686	T4514	T4511	amod	forward,primer
R2687	T4515	T4498	auxpass	was,carried
R2688	T4516	T4498	advmod	then,carried
R2689	T4517	T4498	prt	out,carried
R2690	T4518	T4519	aux	to,amplify
R2691	T4519	T4498	advcl	amplify,carried
R2692	T4520	T4521	det	the,gene
R2693	T4521	T4519	dobj	gene,amplify
R2694	T4522	T4521	amod	specific,gene
R2695	T4523	T4521	compound	mouse,gene
R2696	T4524	T4521	prep	from,gene
R2697	T4525	T4526	det	the,products
R2698	T4526	T4524	pobj	products,from
R2699	T4527	T4528	amod	first,round
R2700	T4528	T4526	compound	round,products
R2701	T4529	T4526	compound	PCR,products
R2702	T4530	T4519	prep	at,amplify
R2703	T4531	T4532	amod	high,temperature
R2704	T4532	T4530	pobj	temperature,at
R2705	T4533	T4532	amod	annealing,temperature
R2706	T4534	T4532	punct	(,temperature
R2707	T4535	T4536	nummod	62,°C
R2708	T4536	T4532	appos	°C,temperature
R2709	T4537	T4498	punct	),carried
R2710	T4538	T4498	punct	.,carried
R2711	T4540	T4541	det	The,products
R2712	T4541	T4544	nsubjpass	products,excised
R2713	T4542	T4541	amod	expected,products
R2714	T4543	T4541	compound	PCR,products
R2715	T4545	T4544	auxpass	were,excised
R2716	T4546	T4544	advmod	directly,excised
R2717	T4547	T4544	prep	from,excised
R2718	T4548	T4549	compound	agarose,gel
R2719	T4549	T4547	pobj	gel,from
R2720	T4550	T4544	cc	and,excised
R2721	T4551	T4544	conj	sequenced,excised
R2722	T4552	T4551	prep	by,sequenced
R2723	T4553	T4554	det	an,sequencer
R2724	T4554	T4552	pobj	sequencer,by
R2725	T4555	T4554	nmod	ABI377,sequencer
R2726	T4556	T4554	amod	automatic,sequencer
R2727	T4557	T4544	punct	.,excised
R2728	T4559	T4560	det	The,sequence
R2729	T4560	T4561	nsubjpass	sequence,confirmed
R2730	T4562	T4561	auxpass	was,confirmed
R2731	T4563	T4561	advmod	further,confirmed
R2732	T4564	T4561	advcl	using,confirmed
R2733	T4565	T4566	det	a,primer
R2734	T4566	T4564	dobj	primer,using
R2735	T4567	T4566	amod	forward,primer
R2736	T4568	T4566	prep	from,primer
R2737	T4569	T4570	advmod	newly,identified
R2738	T4570	T4571	amod	identified,sequence
R2739	T4571	T4568	pobj	sequence,from
R2740	T4572	T4566	cc	and,primer
R2741	T4573	T4574	det	a,primer
R2742	T4574	T4566	conj	primer,primer
R2743	T4575	T4574	amod	reverse,primer
R2744	T4576	T4574	prep	from,primer
R2745	T4577	T4578	amod	known,sequence
R2746	T4578	T4576	pobj	sequence,from
R2747	T4579	T4561	punct	.,confirmed
R2748	T4581	T4582	mark	Once,identified
R2749	T4582	T4589	advcl	identified,obtained
R2750	T4583	T4582	nsubjpass	most,identified
R2751	T4584	T4583	prep	of,most
R2752	T4585	T4586	det	the,sequences
R2753	T4586	T4584	pobj	sequences,of
R2754	T4587	T4586	amod	coding,sequences
R2755	T4588	T4582	auxpass	were,identified
R2756	T4590	T4589	punct	", ",obtained
R2757	T4591	T4592	amod	partial,sequence
R2758	T4592	T4589	nsubjpass	sequence,obtained
R2759	T4593	T4592	prep	of,sequence
R2760	T4594	T4595	nmod	exon,sequences
R2761	T4595	T4593	pobj	sequences,of
R2762	T4596	T4594	nummod	1,exon
R2763	T4597	T4594	cc	and,exon
R2764	T4598	T4599	det	the,UTR
R2765	T4599	T4594	conj	UTR,exon
R2766	T4600	T4599	nummod	5,UTR
R2767	T4601	T4600	punct	',5
R2768	T4602	T4589	auxpass	were,obtained
R2769	T4603	T4589	prep	by,obtained
R2770	T4604	T4605	compound	BAC,sequencing
R2771	T4605	T4603	pobj	sequencing,by
R2772	T4606	T4605	compound	DNA,sequencing
R2773	T4607	T4589	punct	.,obtained
R2774	T4647	T4648	compound	Northern,blot
R2775	T4648	T4649	compound	blot,analyses
R2776	T4651	T4652	amod	Multiple,filters
R2777	T4652	T4656	nsubjpass	filters,purchased
R2778	T4653	T4652	compound	Choice,filters
R2779	T4654	T4655	compound	Northern,Blot
R2780	T4655	T4652	compound	Blot,filters
R2781	T4657	T4652	acl	containing,filters
R2782	T4658	T4659	nummod	12,tissues
R2783	T4659	T4657	dobj	tissues,containing
R2784	T4660	T4659	amod	different,tissues
R2785	T4661	T4659	compound	mouse,tissues
R2786	T4662	T4656	auxpass	were,purchased
R2787	T4663	T4656	prep	from,purchased
R2788	T4664	T4663	pobj	Origene,from
R2789	T4665	T4656	punct	.,purchased
R2790	T4667	T4668	det	The,filters
R2791	T4668	T4669	nsubjpass	filters,probed
R2792	T4670	T4669	auxpass	were,probed
R2793	T4671	T4669	prep	for,probed
R2794	T4672	T4673	det	each,gene
R2795	T4673	T4671	pobj	gene,for
R2796	T4674	T4673	compound	Acdp,gene
R2797	T4675	T4669	prep	with,probed
R2798	T4676	T4677	det	a,fragment
R2799	T4677	T4675	pobj	fragment,with
R2800	T4678	T4677	compound	PCR,fragment
R2801	T4679	T4680	punct	(,bp
R2802	T4680	T4677	parataxis	bp,fragment
R2803	T4681	T4682	quantmod	around,350
R2804	T4682	T4680	nummod	350,bp
R2805	T4683	T4680	punct	),bp
R2806	T4684	T4677	prep	from,fragment
R2807	T4685	T4686	amod	last,exon
R2808	T4686	T4684	pobj	exon,from
R2809	T4687	T4669	cc	and,probed
R2810	T4688	T4689	det	the,3
R2811	T4689	T4690	nummod	3,region
R2812	T4690	T4693	nsubj	region,labeled
R2813	T4691	T4689	punct	',3
R2814	T4692	T4689	amod	untranslated,3
R2815	T4693	T4669	conj	labeled,probed
R2816	T4694	T4693	prep	with,labeled
R2817	T4695	T4696	compound	α,32P
R2818	T4696	T4698	compound	32P,dCTP
R2819	T4697	T4696	punct	-,32P
R2820	T4698	T4694	pobj	dCTP,with
R2821	T4699	T4693	advcl	using,labeled
R2822	T4700	T4701	det	the,system
R2823	T4701	T4699	dobj	system,using
R2824	T4702	T4701	amod	random,system
R2825	T4703	T4701	compound	primer,system
R2826	T4704	T4701	compound	extension,system
R2827	T4705	T4706	punct	(,Technologies
R2828	T4706	T4701	parataxis	Technologies,system
R2829	T4707	T4706	compound	Life,Technologies
R2830	T4708	T4706	punct	),Technologies
R2831	T4709	T4693	punct	.,labeled
R2832	T4711	T4712	nsubjpass	Hybridizations,carried
R2833	T4713	T4712	auxpass	were,carried
R2834	T4714	T4712	prt	out,carried
R2835	T4715	T4712	advmod	overnight,carried
R2836	T4716	T4712	punct	.,carried
R2837	T4718	T4719	det	The,filters
R2838	T4719	T4720	nsubjpass	filters,washed
R2839	T4721	T4720	auxpass	were,washed
R2840	T4722	T4720	advmod	twice,washed
R2841	T4723	T4720	prep	with,washed
R2842	T4724	T4725	amod	washing,buffer
R2843	T4725	T4723	pobj	buffer,with
R2844	T4726	T4725	nummod	I,buffer
R2845	T4727	T4728	punct	(,SDS
R2846	T4728	T4725	parataxis	SDS,buffer
R2847	T4729	T4730	nummod	2,SSC
R2848	T4730	T4728	dep	SSC,SDS
R2849	T4731	T4730	punct	×,SSC
R2850	T4732	T4728	punct	", ",SDS
R2851	T4733	T4734	nummod	0.1,%
R2852	T4734	T4728	compound	%,SDS
R2853	T4735	T4728	punct	),SDS
R2854	T4736	T4720	prep	at,washed
R2855	T4737	T4738	nummod	42,°C
R2856	T4738	T4736	pobj	°C,at
R2857	T4739	T4720	prep	for,washed
R2858	T4740	T4741	nummod	15,min
R2859	T4741	T4739	pobj	min,for
R2860	T4742	T4741	punct	-,min
R2861	T4743	T4720	punct	", ",washed
R2862	T4744	T4720	cc	and,washed
R2863	T4745	T4746	advmod	then,washed
R2864	T4746	T4720	conj	washed,washed
R2865	T4747	T4746	advmod	twice,washed
R2866	T4748	T4746	prep	with,washed
R2867	T4749	T4750	compound	washing,buffer
R2868	T4750	T4748	pobj	buffer,with
R2869	T4751	T4750	nummod	II,buffer
R2870	T4752	T4753	punct	(,SDS
R2871	T4753	T4750	parataxis	SDS,buffer
R2872	T4754	T4755	nummod	0.25,SSC
R2873	T4755	T4753	dep	SSC,SDS
R2874	T4756	T4755	punct	×,SSC
R2875	T4757	T4753	punct	", ",SDS
R2876	T4758	T4759	nummod	0.1,%
R2877	T4759	T4753	compound	%,SDS
R2878	T4760	T4753	punct	),SDS
R2879	T4761	T4746	prep	at,washed
R288	T593	T594	nsubj	We,cloned
R2880	T4762	T4763	nummod	65,°C
R2881	T4763	T4761	pobj	°C,at
R2882	T4764	T4746	prep	for,washed
R2883	T4765	T4766	nummod	15,min
R2884	T4766	T4764	pobj	min,for
R2885	T4767	T4766	punct	-,min
R2886	T4768	T4720	punct	.,washed
R2887	T4770	T4771	amod	Washed,filters
R2888	T4771	T4772	nsubjpass	filters,exposed
R2889	T4773	T4772	auxpass	were,exposed
R289	T595	T594	aux	have,cloned
R2890	T4774	T4772	prep	to,exposed
R2891	T4775	T4776	compound	X-ray,films
R2892	T4776	T4774	pobj	films,to
R2893	T4777	T4772	prep	for,exposed
R2894	T4778	T4777	pcomp	overnight,for
R2895	T4779	T4778	cc	or,overnight
R2896	T4780	T4778	conj	longer,overnight
R2897	T4781	T4782	punct	[,4
R2898	T4782	T4772	parataxis	4,exposed
R2899	T4783	T4782	nummod	3,4
R290	T596	T594	advmod	recently,cloned
R2900	T4784	T4782	punct	",",4
R2901	T4785	T4782	punct	],4
R2902	T4786	T4772	punct	.,exposed
R2904	T4823	T4824	compound	Antibody,production
R2905	T4825	T4824	cc	and,production
R2906	T4826	T4827	compound	Western,blot
R2907	T4827	T4828	compound	blot,analyses
R2908	T4828	T4824	conj	analyses,production
R2909	T4830	T4831	nsubjpass	Peptides,used
R291	T597	T594	cc	and,cloned
R2910	T4832	T4830	acl	linked,Peptides
R2911	T4833	T4832	prep	to,linked
R2912	T4834	T4833	pobj	KLH,to
R2913	T4835	T4834	punct	(,KLH
R2914	T4836	T4837	compound	keyhole,hemacyanin
R2915	T4837	T4834	appos	hemacyanin,KLH
R2916	T4838	T4837	compound	limpet,hemacyanin
R2917	T4839	T4833	punct	),to
R2918	T4840	T4833	prep	from,to
R2919	T4841	T4842	nmod	Acdp1,terminals
R292	T598	T594	conj	characterized,cloned
R2920	T4842	T4840	pobj	terminals,from
R2921	T4843	T4842	nmod	N,terminals
R2922	T4844	T4843	punct	-,N
R2923	T4845	T4843	cc	and,N
R2924	T4846	T4843	conj	C,N
R2925	T4847	T4842	punct	-,terminals
R2926	T4848	T4842	cc	and,terminals
R2927	T4849	T4850	det	the,domain
R2928	T4850	T4842	conj	domain,terminals
R2929	T4851	T4850	amod	conserved,domain
R293	T599	T600	det	a,family
R2930	T4852	T4850	punct	(,domain
R2931	T4853	T4850	appos	ACD,domain
R2932	T4854	T4831	punct	),used
R2933	T4855	T4831	auxpass	were,used
R2934	T4856	T4831	prep	for,used
R2935	T4857	T4856	pobj	generation,for
R2936	T4858	T4857	prep	of,generation
R2937	T4859	T4858	pobj	antibodies,of
R2938	T4860	T4861	advmod	specifically,for
R2939	T4861	T4856	prep	for,for
R294	T600	T598	dobj	family,characterized
R2940	T4862	T4861	pobj	Acdp1,for
R2941	T4863	T4862	cc	and,Acdp1
R2942	T4864	T4865	det	all,members
R2943	T4865	T4862	conj	members,Acdp1
R2944	T4866	T4865	compound	Acdp,members
R2945	T4867	T4868	mark	as,reported
R2946	T4868	T4831	advcl	reported,used
R2947	T4869	T4831	punct	", ",used
R2948	T4870	T4831	advmod	respectively,used
R2949	T4871	T4872	punct	[,5
R295	T601	T600	amod	novel,family
R2950	T4872	T4831	parataxis	5,used
R2951	T4873	T4872	punct	],5
R2952	T4874	T4831	punct	.,used
R2953	T4876	T4877	compound	Western,blots
R2954	T4877	T4878	nsubjpass	blots,carried
R2955	T4879	T4878	auxpass	were,carried
R2956	T4880	T4878	prt	out,carried
R2957	T4881	T4878	advcl	Using,carried
R2958	T4882	T4881	dobj	ECL,Using
R2959	T4883	T4884	punct	(,PIERCE
R296	T602	T600	compound	gene,family
R2960	T4884	T4882	parataxis	PIERCE,ECL
R2961	T4885	T4884	punct	),PIERCE
R2962	T4886	T4887	mark	as,described
R2963	T4887	T4878	advcl	described,carried
R2964	T4888	T4887	advmod	previously,described
R2965	T4889	T4890	punct	[,1
R2966	T4890	T4878	parataxis	1,carried
R2967	T4891	T4890	punct	],1
R2968	T4892	T4878	punct	.,carried
R2969	T4894	T4895	det	The,membranes
R297	T603	T600	acl	named,family
R2970	T4895	T4896	nsubjpass	membranes,washed
R2971	T4897	T4896	auxpass	were,washed
R2972	T4898	T4896	advmod	extensively,washed
R2973	T4899	T4896	prep	after,washed
R2974	T4900	T4899	pobj	incubation,after
R2975	T4901	T4900	prep	with,incubation
R2976	T4902	T4903	amod	primary,antibodies
R2977	T4903	T4901	pobj	antibodies,with
R2978	T4904	T4902	cc	and,primary
R2979	T4905	T4902	conj	secondary,primary
R298	T604	T605	amod	ancient,protein
R2980	T4906	T4896	cc	and,washed
R2981	T4907	T4908	auxpass	were,developed
R2982	T4908	T4896	conj	developed,washed
R2983	T4909	T4908	advmod	then,developed
R2984	T4910	T4908	prep	with,developed
R2985	T4911	T4912	compound	X-ray,films
R2986	T4912	T4910	pobj	films,with
R2987	T4913	T4912	prep	with,films
R2988	T4914	T4915	amod	optimal,time
R2989	T4915	T4913	pobj	time,with
R299	T605	T603	oprd	protein,named
R2990	T4916	T4915	compound	exposure,time
R2991	T4917	T4896	punct	.,washed
R2992	T4941	T4942	compound	Chromosome,localization
R2993	T4944	T4945	det	The,panel
R2994	T4945	T4950	nsubjpass	panel,used
R2995	T4946	T4945	compound	T31,panel
R2996	T4947	T4945	compound	mouse,panel
R2997	T4948	T4945	compound	radiation,panel
R2998	T4949	T4945	compound	hybrid,panel
R2999	T4951	T4945	prep	from,panel
R300	T606	T605	amod	conserved,protein
R3000	T4952	T4953	compound	Research,Genetics
R3001	T4953	T4951	pobj	Genetics,from
R3002	T4954	T4950	auxpass	was,used
R3003	T4955	T4956	aux	to,map
R3004	T4956	T4950	advcl	map,used
R3005	T4957	T4958	det	the,location
R3006	T4958	T4956	dobj	location,map
R3007	T4959	T4958	compound	chromosome,location
R3008	T4960	T4958	prep	of,location
R3009	T4961	T4962	det	each,member
R301	T607	T605	compound	domain,protein
R3010	T4962	T4960	pobj	member,of
R3011	T4963	T4962	compound	Acdp,member
R3012	T4964	T4950	punct	.,used
R3013	T4966	T4967	nsubjpass	Primers,used
R3014	T4968	T4966	prep	from,Primers
R3015	T4969	T4970	nummod	3,UTR
R3016	T4970	T4968	pobj	UTR,from
R3017	T4971	T4969	punct	',3
R3018	T4972	T4970	prep	of,UTR
R3019	T4973	T4974	det	each,member
R302	T608	T605	punct	(,protein
R3020	T4974	T4972	pobj	member,of
R3021	T4975	T4974	compound	Acdp,member
R3022	T4976	T4967	auxpass	were,used
R3023	T4977	T4978	aux	to,amplify
R3024	T4978	T4967	advcl	amplify,used
R3025	T4979	T4980	det	the,clones
R3026	T4980	T4978	dobj	clones,amplify
R3027	T4981	T4980	nummod	100,clones
R3028	T4982	T4980	nmod	radiation,clones
R3029	T4983	T4980	amod	hybrid,clones
R303	T609	T605	appos	ACDP,protein
R3030	T4984	T4980	acl	representing,clones
R3031	T4985	T4986	det	the,genome
R3032	T4986	T4984	dobj	genome,representing
R3033	T4987	T4986	compound	mouse,genome
R3034	T4988	T4967	punct	.,used
R3035	T4990	T4991	det	The,data
R3036	T4991	T4992	nsubjpass	data,submitted
R3037	T4993	T4992	auxpass	were,submitted
R3038	T4994	T4992	prep	to,submitted
R3039	T4995	T4996	det	the,Database
R304	T610	T605	punct	),protein
R3040	T4996	T4994	pobj	Database,to
R3041	T4997	T4998	compound	Jackson,Laboratory
R3042	T4998	T4996	compound	Laboratory,Database
R3043	T4999	T5000	compound	Mouse,Hybrid
R3044	T5000	T4996	compound	Hybrid,Database
R3045	T5001	T5000	compound	Radiation,Hybrid
R3046	T5002	T4992	prep	for,submitted
R3047	T5003	T5002	pobj	analysis,for
R3048	T5004	T4992	punct	.,submitted
R3049	T5023	T5024	compound	Sequence,analyses
R305	T611	T612	dep	which,encodes
R3050	T5026	T5027	compound	Sequence,assembly
R3051	T5027	T5028	nsubjpass	assembly,performed
R3052	T5029	T5028	auxpass	was,performed
R3053	T5030	T5028	prep	with,performed
R3054	T5031	T5032	compound	program,Sequencher
R3055	T5032	T5030	pobj	Sequencher,with
R3056	T5033	T5034	punct	(,Corp
R3057	T5034	T5032	parataxis	Corp,Sequencher
R3058	T5035	T5034	compound	Gene,Corp
R3059	T5036	T5034	compound	Codes,Corp
R306	T612	T600	relcl	encodes,family
R3060	T5037	T5034	punct	),Corp
R3061	T5038	T5028	punct	.,performed
R3062	T5040	T5041	nmod	Protein,homology
R3063	T5041	T5044	compound	homology,searches
R3064	T5042	T5040	cc	and,Protein
R3065	T5043	T5040	conj	DNA,Protein
R3066	T5044	T5045	nsubjpass	searches,carried
R3067	T5046	T5045	auxpass	were,carried
R3068	T5047	T5045	prt	out,carried
R3069	T5048	T5045	prep	with,carried
R307	T613	T614	nummod	four,members
R3070	T5049	T5050	nmod	tblastn,programs
R3071	T5050	T5048	pobj	programs,with
R3072	T5051	T5049	punct	", ",tblastn
R3073	T5052	T5049	conj	tblastx,tblastn
R3074	T5053	T5052	punct	", ",tblastx
R3075	T5054	T5052	conj	blastp,tblastx
R3076	T5055	T5054	cc	and,blastp
R3077	T5056	T5054	conj	blastn,blastp
R3078	T5057	T5045	punct	.,carried
R3079	T5059	T5060	amod	Multiple,alignments
R308	T614	T612	dobj	members,encodes
R3080	T5060	T5062	nsubjpass	alignments,performed
R3081	T5061	T5060	compound	sequence,alignments
R3082	T5063	T5062	auxpass	were,performed
R3083	T5064	T5062	prep	with,performed
R3084	T5065	T5066	nmod	GeneDoc,alignment
R3085	T5066	T5064	pobj	alignment,with
R3086	T5067	T5065	cc	and,GeneDoc
R3087	T5068	T5065	conj	pairwise,GeneDoc
R3088	T5069	T5066	compound	sequence,alignment
R3089	T5070	T5062	punct	.,performed
R309	T615	T614	compound	protein,members
R3090	T5072	T5073	amod	Multiple,programs
R3091	T5073	T5074	nsubjpass	programs,used
R3092	T5075	T5073	prep	including,programs
R3093	T5076	T5077	compound	BCM,Launcher
R3094	T5077	T5075	pobj	Launcher,including
R3095	T5078	T5077	compound	Search,Launcher
R3096	T5079	T5077	punct	", ",Launcher
R3097	T5080	T5077	conj	ProfileScan,Launcher
R3098	T5081	T5080	punct	", ",ProfileScan
R3099	T5082	T5083	compound	sequence,search
R310	T616	T612	prep	in,encodes
R3100	T5083	T5080	conj	search,ProfileScan
R3101	T5084	T5083	compound	motif,search
R3102	T5085	T5083	punct	", ",search
R3103	T5086	T5083	conj	ExPASy,search
R3104	T5087	T5086	cc	and,ExPASy
R3105	T5088	T5086	conj	3Dpssm,ExPASy
R3106	T5089	T5074	auxpass	were,used
R3107	T5090	T5074	prep	for,used
R3108	T5091	T5090	pcomp	searching,for
R3109	T5092	T5093	compound	sequence,features
R311	T617	T616	pobj	humans,in
R3110	T5093	T5091	dobj	features,searching
R3111	T5094	T5093	prep	of,features
R3112	T5095	T5096	amod	known,protein
R3113	T5096	T5094	pobj	protein,of
R3114	T5097	T5074	punct	.,used
R3115	T5099	T5100	amod	Phylogenetic,tree
R3116	T5100	T5101	nsubjpass	tree,constructed
R3117	T5102	T5101	auxpass	was,constructed
R3118	T5103	T5101	agent	by,constructed
R3119	T5104	T5105	compound	Clustalw,program
R312	T618	T619	punct	[,1
R3120	T5105	T5103	pobj	program,by
R3121	T5106	T5107	punct	(,version
R3122	T5107	T5105	parataxis	version,program
R3123	T5108	T5107	nummod	1.81,version
R3124	T5109	T5107	punct	),version
R3125	T5110	T5101	advcl	using,constructed
R3126	T5111	T5112	nmod	UPGMA,algorithm
R3127	T5112	T5110	dobj	algorithm,using
R3128	T5113	T5111	punct	(,UPGMA
R3129	T5114	T5115	amod	Unweighted,Method
R313	T619	T598	parataxis	1,characterized
R3130	T5115	T5111	appos	Method,UPGMA
R3131	T5116	T5115	compound	Pair,Method
R3132	T5117	T5115	compound	Group,Method
R3133	T5118	T5115	acl	using,Method
R3134	T5119	T5120	compound	Arithmetic,averages
R3135	T5120	T5118	dobj	averages,using
R3136	T5121	T5112	punct	),algorithm
R3137	T5122	T5123	punct	[,14
R3138	T5123	T5101	parataxis	14,constructed
R3139	T5124	T5123	punct	],14
R314	T620	T619	punct	],1
R3140	T5125	T5101	punct	.,constructed
R3141	T5264	T5265	amod	Neuronal,preparation
R3142	T5266	T5265	compound	cell,preparation
R3143	T5267	T5265	cc	and,preparation
R3144	T5268	T5265	conj	immunostanining,preparation
R3145	T5270	T5271	amod	Hippocampal,cultures
R3146	T5271	T5273	nsubjpass	cultures,prepared
R3147	T5272	T5271	compound	neuron,cultures
R3148	T5274	T5273	auxpass	were,prepared
R3149	T5275	T5276	mark	as,reported
R315	T621	T594	punct	.,cloned
R3150	T5276	T5273	advcl	reported,prepared
R3151	T5277	T5276	advmod	previously,reported
R3152	T5278	T5279	punct	[,6
R3153	T5279	T5273	parataxis	6,prepared
R3154	T5280	T5279	punct	],6
R3155	T5281	T5273	punct	.,prepared
R3156	T5283	T5284	prep	In,dissected
R3157	T5285	T5283	pobj	brief,In
R3158	T5286	T5284	punct	", ",dissected
R3159	T5287	T5288	det	the,hippocampuses
R316	T623	T624	nsubj	We,found
R3160	T5288	T5284	nsubjpass	hippocampuses,dissected
R3161	T5289	T5284	auxpass	were,dissected
R3162	T5290	T5284	prt	out,dissected
R3163	T5291	T5284	prep	from,dissected
R3164	T5292	T5293	compound	mouse,embryos
R3165	T5293	T5291	pobj	embryos,from
R3166	T5294	T5284	prep	at,dissected
R3167	T5295	T5296	nummod	16,days
R3168	T5296	T5294	pobj	days,at
R3169	T5297	T5298	advmod	in,utero
R317	T625	T626	mark	that,is
R3170	T5298	T5296	advmod	utero,days
R3171	T5299	T5284	punct	.,dissected
R3172	T5301	T5302	det	The,tissues
R3173	T5302	T5303	nsubjpass	tissues,incubated
R3174	T5304	T5303	auxpass	were,incubated
R3175	T5305	T5303	advmod	then,incubated
R3176	T5306	T5303	prep	for,incubated
R3177	T5307	T5308	nummod	20,min
R3178	T5308	T5306	pobj	min,for
R3179	T5309	T5303	prep	at,incubated
R318	T626	T624	ccomp	is,found
R3180	T5310	T5311	nummod	37,°C
R3181	T5311	T5309	pobj	°C,at
R3182	T5312	T5303	prep	in,incubated
R3183	T5313	T5312	pobj	MEM,in
R3184	T5314	T5313	punct	(,MEM
R3185	T5315	T5316	amod	minimum,medium
R3186	T5316	T5313	appos	medium,MEM
R3187	T5317	T5316	amod	essential,medium
R3188	T5318	T5313	punct	),MEM
R3189	T5319	T5313	acl	modified,MEM
R319	T627	T628	det	this,family
R3190	T5320	T5319	prep	for,modified
R3191	T5321	T5322	compound	suspension,culture
R3192	T5322	T5320	pobj	culture,for
R3193	T5323	T5324	punct	(,Technologies
R3194	T5324	T5313	parataxis	Technologies,MEM
R3195	T5325	T5324	compound	Life,Technologies
R3196	T5326	T5324	punct	),Technologies
R3197	T5327	T5313	cc	plus,MEM
R3198	T5328	T5329	nummod	0.25,%
R3199	T5329	T5330	compound	%,trypsin
R320	T628	T626	nsubj	family,is
R3200	T5330	T5313	conj	trypsin,MEM
R3201	T5331	T5332	punct	(,Technologies
R3202	T5332	T5330	parataxis	Technologies,trypsin
R3203	T5333	T5332	compound	Life,Technologies
R3204	T5334	T5332	punct	),Technologies
R3205	T5335	T5303	punct	.,incubated
R3206	T5337	T5338	det	The,neurons
R3207	T5338	T5341	nsubjpass	neurons,plated
R3208	T5339	T5338	amod	dissociated,neurons
R3209	T5340	T5338	amod	hippocampal,neurons
R321	T629	T628	compound	gene,family
R3210	T5342	T5341	auxpass	were,plated
R3211	T5343	T5341	prep	on,plated
R3212	T5344	T5345	compound	glass,coverslips
R3213	T5345	T5343	pobj	coverslips,on
R3214	T5346	T5345	acl	coated,coverslips
R3215	T5347	T5346	prep	with,coated
R3216	T5348	T5349	det	a,monolayer
R3217	T5349	T5347	pobj	monolayer,with
R3218	T5350	T5349	amod	confluent,monolayer
R3219	T5351	T5349	prep	of,monolayer
R322	T630	T631	advmod	evolutionarily,conserved
R3220	T5352	T5353	nmod	mouse,astrocytes
R3221	T5353	T5351	pobj	astrocytes,of
R3222	T5354	T5353	amod	cortical,astrocytes
R3223	T5355	T5353	acl	obtained,astrocytes
R3224	T5356	T5357	mark	as,described
R3225	T5357	T5355	advcl	described,obtained
R3226	T5358	T5357	advmod	below,described
R3227	T5359	T5341	punct	.,plated
R3228	T5361	T5362	det	The,neurons
R3229	T5362	T5363	nsubjpass	neurons,maintained
R323	T631	T626	acomp	conserved,is
R3230	T5364	T5363	auxpass	were,maintained
R3231	T5365	T5363	prep	at,maintained
R3232	T5366	T5367	nummod	37,°C
R3233	T5367	T5365	pobj	°C,at
R3234	T5368	T5363	prep	in,maintained
R3235	T5369	T5370	det	a,atmosphere
R3236	T5370	T5368	pobj	atmosphere,in
R3237	T5371	T5370	amod	humidified,atmosphere
R3238	T5372	T5370	prep	with,atmosphere
R3239	T5373	T5374	nummod	5,%
R324	T632	T626	prep	in,is
R3240	T5374	T5375	compound	%,CO2
R3241	T5375	T5372	pobj	CO2,with
R3242	T5376	T5363	punct	.,maintained
R3243	T5378	T5379	amod	Cortical,astrocytes
R3244	T5379	T5380	nsubjpass	astrocytes,grown
R3245	T5381	T5379	acl	dissociated,astrocytes
R3246	T5382	T5381	prep	from,dissociated
R3247	T5383	T5384	amod	newborn,mouse
R3248	T5384	T5385	compound	mouse,cortices
R3249	T5385	T5382	pobj	cortices,from
R325	T633	T634	amod	diverse,species
R3250	T5386	T5380	auxpass	were,grown
R3251	T5387	T5380	prep	in,grown
R3252	T5388	T5389	compound	culture,flasks
R3253	T5389	T5387	pobj	flasks,in
R3254	T5390	T5380	prep	at,grown
R3255	T5391	T5392	nummod	37,°C
R3256	T5392	T5390	pobj	°C,at
R3257	T5393	T5380	prep	in,grown
R3258	T5394	T5395	det	a,atmosphere
R3259	T5395	T5393	pobj	atmosphere,in
R326	T634	T632	pobj	species,in
R3260	T5396	T5395	amod	humidified,atmosphere
R3261	T5397	T5395	prep	with,atmosphere
R3262	T5398	T5399	nummod	5,%
R3263	T5399	T5400	compound	%,CO2
R3264	T5400	T5397	pobj	CO2,with
R3265	T5401	T5380	prep	until,grown
R3266	T5402	T5401	pcomp	confluent,until
R3267	T5403	T5380	punct	.,grown
R3268	T5405	T5406	det	The,cells
R3269	T5406	T5407	nsubjpass	cells,exposed
R327	T635	T634	acl	ranging,species
R3270	T5408	T5407	auxpass	were,exposed
R3271	T5409	T5407	prep	to,exposed
R3272	T5410	T5411	quantmod	10,5
R3273	T5411	T5413	nummod	5,M
R3274	T5412	T5411	punct	-,5
R3275	T5413	T5414	compound	M,arabinoside
R3276	T5414	T5409	pobj	arabinoside,to
R3277	T5415	T5414	compound	cytosine,arabinoside
R3278	T5416	T5417	punct	(,Sigma
R3279	T5417	T5414	parataxis	Sigma,arabinoside
R328	T636	T635	prep	from,ranging
R3280	T5418	T5417	punct	),Sigma
R3281	T5419	T5407	cc	and,exposed
R3282	T5420	T5407	conj	cultured,exposed
R3283	T5421	T5420	prep	for,cultured
R3284	T5422	T5423	amod	additional,hrs
R3285	T5423	T5421	pobj	hrs,for
R3286	T5424	T5425	quantmod	12,24
R3287	T5425	T5423	nummod	24,hrs
R3288	T5426	T5425	punct	–,24
R3289	T5427	T5420	prep	at,cultured
R329	T637	T636	pobj	bacteria,from
R3290	T5428	T5429	nummod	37,°C
R3291	T5429	T5427	pobj	°C,at
R3292	T5430	T5407	punct	.,exposed
R3293	T5432	T5433	prep	After,used
R3294	T5434	T5432	pobj	remove,After
R3295	T5435	T5434	prep	of,remove
R3296	T5436	T5437	det	the,media
R3297	T5437	T5435	pobj	media,of
R3298	T5438	T5434	prep	with,remove
R3299	T5439	T5440	amod	cellular,debris
R330	T638	T637	punct	", ",bacteria
R3300	T5440	T5438	pobj	debris,with
R3301	T5441	T5433	punct	", ",used
R3302	T5442	T5443	det	the,cells
R3303	T5443	T5433	nsubjpass	cells,used
R3304	T5444	T5433	auxpass	were,used
R3305	T5445	T5433	prep	for,used
R3306	T5446	T5445	pcomp	coating,for
R3307	T5447	T5446	dobj	coverslips,coating
R3308	T5448	T5433	punct	.,used
R3309	T5450	T5451	prep	For,fixed
R331	T639	T637	conj	yeast,bacteria
R3310	T5452	T5450	pobj	immunostaining,For
R3311	T5453	T5451	punct	", ",fixed
R3312	T5454	T5455	amod	neuronal,cells
R3313	T5455	T5451	nsubjpass	cells,fixed
R3314	T5456	T5455	prep	on,cells
R3315	T5457	T5458	det	the,coverslips
R3316	T5458	T5456	pobj	coverslips,on
R3317	T5459	T5451	auxpass	were,fixed
R3318	T5460	T5451	advmod	first,fixed
R3319	T5461	T5451	prep	in,fixed
R332	T640	T639	punct	", ",yeast
R3320	T5462	T5461	pobj	PBS,in
R3321	T5463	T5462	acl	containing,PBS
R3322	T5464	T5465	nummod	4,%
R3323	T5465	T5466	compound	%,paraformaldehyde
R3324	T5466	T5463	dobj	paraformaldehyde,containing
R3325	T5467	T5466	punct	(,paraformaldehyde
R3326	T5468	T5466	appos	PFA,paraformaldehyde
R3327	T5469	T5463	punct	),containing
R3328	T5470	T5463	prep	for,containing
R3329	T5471	T5472	nummod	12,hrs
R333	T641	T642	compound	C.,elegans
R3330	T5472	T5470	pobj	hrs,for
R3331	T5473	T5463	prep	at,containing
R3332	T5474	T5475	nummod	4,°C
R3333	T5475	T5473	pobj	°C,at
R3334	T5476	T5451	cc	and,fixed
R3335	T5477	T5478	advmod	then,incubated
R3336	T5478	T5451	conj	incubated,fixed
R3337	T5479	T5478	prep	in,incubated
R3338	T5480	T5481	det	a,solution
R3339	T5481	T5479	pobj	solution,in
R334	T642	T639	conj	elegans,yeast
R3340	T5482	T5481	acl	containing,solution
R3341	T5483	T5484	nummod	4,%
R3342	T5484	T5485	compound	%,PFA
R3343	T5485	T5482	dobj	PFA,containing
R3344	T5486	T5485	cc	and,PFA
R3345	T5487	T5488	nummod	0.4,%
R3346	T5488	T5489	compound	%,100
R3347	T5489	T5485	conj	100,PFA
R3348	T5490	T5489	compound	Triton,100
R3349	T5491	T5489	compound	X,100
R335	T643	T642	punct	", ",elegans
R3350	T5492	T5489	punct	-,100
R3351	T5493	T5478	prep	at,incubated
R3352	T5494	T5495	nummod	4,°C
R3353	T5495	T5493	pobj	°C,at
R3354	T5496	T5478	prep	for,incubated
R3355	T5497	T5498	nummod	1,hr
R3356	T5498	T5496	pobj	hr,for
R3357	T5499	T5451	punct	.,fixed
R3358	T5501	T5502	prep	After,incubated
R3359	T5503	T5501	pcomp	washing,After
R336	T644	T642	cc	and,elegans
R3360	T5504	T5503	prep	with,washing
R3361	T5505	T5504	pobj	PBS,with
R3362	T5506	T5507	nummod	three,times
R3363	T5507	T5503	npadvmod	times,washing
R3364	T5508	T5502	punct	", ",incubated
R3365	T5509	T5510	det	the,cells
R3366	T5510	T5502	nsubjpass	cells,incubated
R3367	T5511	T5502	auxpass	were,incubated
R3368	T5512	T5502	prep	with,incubated
R3369	T5513	T5514	det	a,solution
R337	T645	T646	compound	D.,melanogaster
R3370	T5514	T5512	pobj	solution,with
R3371	T5515	T5514	amod	blocking,solution
R3372	T5516	T5514	acl	containing,solution
R3373	T5517	T5518	nummod	1,serum
R3374	T5518	T5516	dobj	serum,containing
R3375	T5519	T5520	punct	:,30
R3376	T5520	T5517	prep	30,1
R3377	T5521	T5518	amod	normal,serum
R3378	T5522	T5518	compound	goat,serum
R3379	T5523	T5502	punct	", ",incubated
R338	T646	T642	conj	melanogaster,elegans
R3380	T5524	T5502	cc	and,incubated
R3381	T5525	T5526	advmod	subsequently,incubated
R3382	T5526	T5502	conj	incubated,incubated
R3383	T5527	T5526	prep	with,incubated
R3384	T5528	T5529	det	a,antibody
R3385	T5529	T5527	pobj	antibody,with
R3386	T5530	T5529	nmod	rabbit,antibody
R3387	T5531	T5529	amod	polyclonal,antibody
R3388	T5532	T5529	amod	anti-ACDP,antibody
R3389	T5533	T5534	punct	(,1
R339	T647	T636	prep	to,from
R3390	T5534	T5529	parataxis	1,antibody
R3391	T5535	T5536	punct	:,3000
R3392	T5536	T5534	prep	3000,1
R3393	T5537	T5534	punct	),1
R3394	T5538	T5526	advmod	overnight,incubated
R3395	T5539	T5526	prep	at,incubated
R3396	T5540	T5541	nummod	4,°C
R3397	T5541	T5539	pobj	°C,at
R3398	T5542	T5502	punct	.,incubated
R3399	T5544	T5545	prep	After,incubated
R340	T648	T647	pobj	mammals,to
R3400	T5546	T5547	amod	extensive,washing
R3401	T5547	T5544	pobj	washing,After
R3402	T5548	T5547	prep	with,washing
R3403	T5549	T5550	nummod	1,%
R3404	T5550	T5551	compound	%,serum
R3405	T5551	T5553	compound	serum,solution
R3406	T5552	T5551	compound	goat,serum
R3407	T5553	T5548	pobj	solution,with
R3408	T5554	T5553	compound	PBS,solution
R3409	T5555	T5545	punct	", ",incubated
R341	T649	T624	punct	.,found
R3410	T5556	T5557	det	the,cells
R3411	T5557	T5545	nsubjpass	cells,incubated
R3412	T5558	T5545	auxpass	were,incubated
R3413	T5559	T5545	prep	with,incubated
R3414	T5560	T5561	det	an,antibody
R3415	T5561	T5559	pobj	antibody,with
R3416	T5562	T5561	nmod	Alex,antibody
R3417	T5563	T5562	nummod	488,Alex
R3418	T5564	T5561	amod	conjugated,antibody
R3419	T5565	T5561	amod	secondary,antibody
R342	T651	T652	det	The,conservation
R3420	T5566	T5567	punct	(,Probes
R3421	T5567	T5561	parataxis	Probes,antibody
R3422	T5568	T5567	dep	1,Probes
R3423	T5569	T5570	punct	:,100
R3424	T5570	T5568	prep	100,1
R3425	T5571	T5568	prep	in,1
R3426	T5572	T5573	nummod	1,%
R3427	T5573	T5574	compound	%,serum
R3428	T5574	T5576	compound	serum,solution
R3429	T5575	T5574	compound	goat,serum
R343	T652	T654	nsubj	conservation,imply
R3430	T5576	T5571	pobj	solution,in
R3431	T5577	T5576	compound	PBS,solution
R3432	T5578	T5567	punct	", ",Probes
R3433	T5579	T5567	compound	Molecular,Probes
R3434	T5580	T5567	punct	),Probes
R3435	T5581	T5545	prep	for,incubated
R3436	T5582	T5583	nummod	3,hrs
R3437	T5583	T5581	pobj	hrs,for
R3438	T5584	T5545	prep	at,incubated
R3439	T5585	T5586	compound	room,temperature
R344	T653	T652	compound	sequence,conservation
R3440	T5586	T5584	pobj	temperature,at
R3441	T5587	T5545	punct	.,incubated
R3442	T5589	T5590	prep	Following,slipped
R3443	T5591	T5592	amod	final,washes
R3444	T5592	T5589	pobj	washes,Following
R3445	T5593	T5592	prep	with,washes
R3446	T5594	T5595	nummod	1,%
R3447	T5595	T5596	compound	%,serum
R3448	T5596	T5598	compound	serum,solution
R3449	T5597	T5596	compound	goat,serum
R345	T655	T652	cc	and,conservation
R3450	T5598	T5593	pobj	solution,with
R3451	T5599	T5598	compound	PBS,solution
R3452	T5600	T5590	punct	", ",slipped
R3453	T5601	T5602	det	the,cells
R3454	T5602	T5590	nsubjpass	cells,slipped
R3455	T5603	T5602	amod	neuronal,cells
R3456	T5604	T5602	prep	on,cells
R3457	T5605	T5606	det	the,coverslips
R3458	T5606	T5604	pobj	coverslips,on
R3459	T5607	T5590	auxpass	were,slipped
R346	T656	T657	det	the,presence
R3460	T5608	T5590	dep	cover,slipped
R3461	T5609	T5590	punct	-,slipped
R3462	T5610	T5590	prep	with,slipped
R3463	T5611	T5612	det	a,medium
R3464	T5612	T5610	pobj	medium,with
R3465	T5613	T5614	npadvmod	glycerol,based
R3466	T5614	T5612	amod	based,medium
R3467	T5615	T5614	punct	-,based
R3468	T5616	T5612	amod	anti-photobleach,medium
R3469	T5617	T5590	punct	.,slipped
R347	T657	T652	conj	presence,conservation
R3470	T5619	T5620	det	The,cells
R3471	T5620	T5621	nsubjpass	cells,viewed
R3472	T5622	T5621	auxpass	were,viewed
R3473	T5623	T5621	prep	under,viewed
R3474	T5624	T5625	det	a,microscope
R3475	T5625	T5623	pobj	microscope,under
R3476	T5626	T5625	amod	confocal,microscope
R3477	T5627	T5628	punct	(,Zeiss
R3478	T5628	T5625	parataxis	Zeiss,microscope
R3479	T5629	T5628	compound	Carl,Zeiss
R348	T658	T657	prep	of,presence
R3480	T5630	T5628	punct	),Zeiss
R3481	T5631	T5621	punct	.,viewed
R3482	T5633	T5634	nsubjpass	Images,captured
R3483	T5635	T5634	auxpass	were,captured
R3484	T5636	T5634	prep	with,captured
R3485	T5637	T5638	det	a,camera
R3486	T5638	T5636	pobj	camera,with
R3487	T5639	T5638	compound	CCD,camera
R3488	T5640	T5634	cc	and,captured
R3489	T5641	T5634	conj	acquired,captured
R349	T659	T660	amod	multiple,members
R3490	T5642	T5641	prep	by,acquired
R3491	T5643	T5644	det	the,software
R3492	T5644	T5642	pobj	software,by
R3493	T5645	T5644	compound	Scion,software
R3494	T5646	T5644	compound	Image,software
R3495	T5647	T5648	punct	(,Corporation
R3496	T5648	T5644	parataxis	Corporation,software
R3497	T5649	T5648	compound	Scion,Corporation
R3498	T5650	T5648	punct	", ",Corporation
R3499	T5651	T5648	npadvmod	Frederick,Corporation
R350	T660	T658	pobj	members,of
R3500	T5652	T5648	punct	", ",Corporation
R3501	T5653	T5648	npadvmod	MD,Corporation
R3502	T5654	T5648	punct	),Corporation
R3503	T5655	T5634	punct	.,captured
R3504	T5677	T5678	compound	Gene,bank
R3505	T5678	T5679	compound	bank,number
R3506	T5680	T5679	compound	accession,number
R3507	T5682	T5683	det	The,sequences
R3508	T5683	T5685	nsubjpass	sequences,deposited
R3509	T5684	T5683	compound	cDNA,sequences
R351	T661	T657	prep	within,presence
R3510	T5686	T5683	prep	for,sequences
R3511	T5687	T5688	det	the,family
R3512	T5688	T5686	pobj	family,for
R3513	T5689	T5688	compound	Acdp,family
R3514	T5690	T5688	compound	gene,family
R3515	T5691	T5685	aux	have,deposited
R3516	T5692	T5685	advmod	already,deposited
R3517	T5693	T5685	auxpass	been,deposited
R3518	T5694	T5685	prep	in,deposited
R3519	T5695	T5696	compound	gene,bank
R352	T662	T663	det	a,species
R3520	T5696	T5694	pobj	bank,in
R3521	T5697	T5685	prep	with,deposited
R3522	T5698	T5699	compound	accession,AF202994
R3523	T5699	T5697	pobj	AF202994,with
R3524	T5700	T5699	compound	numbers,AF202994
R3525	T5701	T5699	punct	(,AF202994
R3526	T5702	T5699	appos	Acdp1,AF202994
R3527	T5703	T5699	punct	),AF202994
R3528	T5704	T5699	punct	", ",AF202994
R3529	T5705	T5699	conj	AF216961,AF202994
R353	T663	T661	pobj	species,within
R3530	T5706	T5705	punct	(,AF216961
R3531	T5707	T5705	appos	Acdp2,AF216961
R3532	T5708	T5705	punct	),AF216961
R3533	T5709	T5705	punct	", ",AF216961
R3534	T5710	T5705	conj	AF216964,AF216961
R3535	T5711	T5710	punct	(,AF216964
R3536	T5712	T5710	appos	Acdp3,AF216964
R3537	T5713	T5710	punct	),AF216964
R3538	T5714	T5710	cc	and,AF216964
R3539	T5715	T5710	conj	AF216963,AF216964
R354	T664	T654	aux	may,imply
R3540	T5716	T5715	punct	(,AF216963
R3541	T5717	T5715	appos	Acdp4,AF216963
R3542	T5718	T5699	punct	),AF202994
R3543	T5719	T5685	punct	.,deposited
R3544	T5731	T5732	compound	Northern,blot
R3545	T5732	T5733	compound	blot,analyses
R3546	T5734	T5733	prep	of,analyses
R3547	T5735	T5736	det	the,family
R3548	T5736	T5734	pobj	family,of
R3549	T5737	T5736	compound	Acdp,family
R355	T665	T666	amod	functional,importance
R3550	T5738	T5736	compound	gene,family
R3551	T5739	T5733	punct	.,analyses
R3552	T5741	T5742	compound	S.,muscle
R3553	T5742	T5743	nsubj	muscle,represents
R3554	T5744	T5745	amod	skeletal,muscle
R3555	T5745	T5743	dobj	muscle,represents
R3556	T5746	T5745	punct	", ",muscle
R3557	T5747	T5745	appos	Sm,muscle
R3558	T5748	T5743	punct	.,represents
R3559	T5750	T5751	nsubj	Int.,represents
R356	T666	T654	dobj	importance,imply
R3560	T5752	T5753	amod	small,intestine
R3561	T5753	T5751	dobj	intestine,represents
R3562	T5754	T5751	punct	.,represents
R3563	T5756	T5757	amod	Multiple,Choice
R3564	T5757	T5758	compound	Choice,Blot
R3565	T5758	T5760	compound	Blot,filters
R3566	T5759	T5758	compound	Northern,Blot
R3567	T5760	T5761	nsubjpass	filters,purchased
R3568	T5762	T5761	auxpass	were,purchased
R3569	T5763	T5761	prep	from,purchased
R357	T667	T666	acl	associated,importance
R3570	T5764	T5763	pobj	Origene,from
R3571	T5765	T5761	punct	.,purchased
R3572	T5826	T5827	compound	Amino,acid
R3573	T5827	T5828	compound	acid,sequence
R3574	T5828	T5829	compound	sequence,alignment
R3575	T5830	T5829	compound	homology,alignment
R3576	T5831	T5829	prep	for,alignment
R3577	T5832	T5831	pobj	all,for
R3578	T5833	T5832	prep	of,all
R3579	T5834	T5835	det	the,genes
R358	T668	T667	prep	with,associated
R3580	T5835	T5833	pobj	genes,of
R3581	T5836	T5835	nmod	ACDP,genes
R3582	T5837	T5836	cc	and,ACDP
R3583	T5838	T5836	conj	Acdp,ACDP
R3584	T5839	T5835	prep	within,genes
R3585	T5840	T5841	det	the,domain
R3586	T5841	T5839	pobj	domain,within
R3587	T5842	T5841	compound	ACD,domain
R3588	T5843	T5829	punct	.,alignment
R3589	T5845	T5846	det	The,data
R359	T669	T670	det	the,genes
R3590	T5846	T5848	nsubjpass	data,deposited
R3591	T5847	T5846	compound	sequence,data
R3592	T5849	T5846	prep	for,data
R3593	T5850	T5851	det	the,genes
R3594	T5851	T5849	pobj	genes,for
R3595	T5852	T5851	compound	Acdp,genes
R3596	T5853	T5848	aux	have,deposited
R3597	T5854	T5848	auxpass	been,deposited
R3598	T5855	T5848	prep	in,deposited
R3599	T5856	T5855	pobj	GenBank,in
R360	T670	T668	pobj	genes,with
R3600	T5857	T5848	prep	under,deposited
R3601	T5858	T5859	compound	accession,AF202994
R3602	T5859	T5857	pobj	AF202994,under
R3603	T5860	T5859	compound	number,AF202994
R3604	T5861	T5859	punct	(,AF202994
R3605	T5862	T5859	appos	Acdp1,AF202994
R3606	T5863	T5859	punct	),AF202994
R3607	T5864	T5859	punct	", ",AF202994
R3608	T5865	T5859	conj	AF216961,AF202994
R3609	T5866	T5865	punct	(,AF216961
R361	T671	T654	punct	.,imply
R3610	T5867	T5865	appos	Acdp2,AF216961
R3611	T5868	T5865	punct	),AF216961
R3612	T5869	T5865	punct	", ",AF216961
R3613	T5870	T5865	conj	AF216964,AF216961
R3614	T5871	T5870	punct	(,AF216964
R3615	T5872	T5870	appos	Acdp3,AF216964
R3616	T5873	T5870	punct	),AF216964
R3617	T5874	T5870	cc	and,AF216964
R3618	T5875	T5870	conj	AF216963,AF216964
R3619	T5876	T5875	punct	(,AF216963
R362	T673	T674	aux	To,facilitate
R3620	T5877	T5875	appos	Acdp4,AF216963
R3621	T5878	T5859	punct	),AF202994
R3622	T5879	T5848	punct	.,deposited
R3623	T5881	T5882	amod	Identical,acids
R3624	T5882	T5884	nsubjpass	acids,shaded
R3625	T5883	T5882	compound	amino,acids
R3626	T5885	T5882	cc	or,acids
R3627	T5886	T5887	compound	amino,acids
R3628	T5887	T5882	conj	acids,acids
R3629	T5888	T5887	prep	with,acids
R363	T674	T675	advcl	facilitate,cloned
R3630	T5889	T5890	advmod	very,strong
R3631	T5890	T5891	amod	strong,homologies
R3632	T5891	T5888	pobj	homologies,with
R3633	T5892	T5891	prep	among,homologies
R3634	T5893	T5894	det	all,proteins
R3635	T5894	T5892	pobj	proteins,among
R3636	T5895	T5884	auxpass	were,shaded
R3637	T5896	T5884	oprd	black,shaded
R3638	T5897	T5884	punct	.,shaded
R3639	T5899	T5900	amod	Identical,acids
R364	T676	T677	det	the,analysis
R3640	T5900	T5902	nsubjpass	acids,shaded
R3641	T5901	T5900	compound	amino,acids
R3642	T5903	T5900	cc	or,acids
R3643	T5904	T5905	compound	amino,acids
R3644	T5905	T5900	conj	acids,acids
R3645	T5906	T5905	prep	with,acids
R3646	T5907	T5908	advmod	very,strong
R3647	T5908	T5909	amod	strong,homologies
R3648	T5909	T5906	pobj	homologies,with
R3649	T5910	T5909	prep	in,homologies
R365	T677	T674	dobj	analysis,facilitate
R3650	T5911	T5910	pobj	most,in
R3651	T5912	T5911	prep	of,most
R3652	T5913	T5914	det	the,proteins
R3653	T5914	T5912	pobj	proteins,of
R3654	T5915	T5902	auxpass	were,shaded
R3655	T5916	T5902	oprd	grey,shaded
R3656	T5917	T5902	punct	.,shaded
R3657	T5919	T5920	compound	Dot,lines
R3658	T5920	T5921	nsubj	lines,represent
R3659	T5922	T5921	dobj	gaps,represent
R366	T678	T677	amod	functional,analysis
R3660	T5923	T5922	prep	for,gaps
R3661	T5924	T5925	det	the,alignment
R3662	T5925	T5923	pobj	alignment,for
R3663	T5926	T5921	punct	.,represent
R3664	T6010	T6011	compound	Amino,acid
R3665	T6011	T6012	compound	acid,alignment
R3666	T6013	T6012	compound	sequence,alignment
R3667	T6014	T6012	acl	showing,alignment
R3668	T6015	T6016	det	the,conservation
R3669	T6016	T6014	dobj	conservation,showing
R367	T679	T677	prep	of,analysis
R3670	T6017	T6016	prep	of,conservation
R3671	T6018	T6019	compound	ACD,domain
R3672	T6019	T6017	pobj	domain,of
R3673	T6020	T6014	prep	in,showing
R3674	T6021	T6022	amod	various,species
R3675	T6022	T6020	pobj	species,in
R3676	T6023	T6012	punct	.,alignment
R3677	T6025	T6026	nsubj	Amip3,is
R3678	T6027	T6028	det	a,protein
R3679	T6028	T6026	attr	protein,is
R368	T680	T681	det	the,family
R3680	T6029	T6028	prep	from,protein
R3681	T6030	T6031	compound	Saccharomyces,cerevisiae
R3682	T6031	T6029	pobj	cerevisiae,from
R3683	T6032	T6028	punct	(,protein
R3684	T6033	T6028	appos	NP_014581,protein
R3685	T6034	T6026	punct	),is
R3686	T6035	T6026	punct	.,is
R3687	T6037	T6038	nsubj	CanG,is
R3688	T6039	T6040	det	a,protein
R3689	T6040	T6038	attr	protein,is
R369	T681	T679	pobj	family,of
R3690	T6041	T6040	prep	from,protein
R3691	T6042	T6043	compound	Candida,glabrata
R3692	T6043	T6041	pobj	glabrata,from
R3693	T6044	T6040	punct	(,protein
R3694	T6045	T6040	appos	AAF33142,protein
R3695	T6046	T6038	punct	),is
R3696	T6047	T6038	punct	.,is
R3697	T6049	T6050	nsubj	NeuC,is
R3698	T6051	T6049	punct	(,NeuC
R3699	T6052	T6049	appos	EAA31204,NeuC
R370	T682	T681	compound	ACDP,family
R3700	T6053	T6050	punct	),is
R3701	T6054	T6055	det	a,protein
R3702	T6055	T6050	attr	protein,is
R3703	T6056	T6055	amod	hypothetical,protein
R3704	T6057	T6055	prep	from,protein
R3705	T6058	T6059	compound	Neurospora,crassa
R3706	T6059	T6057	pobj	crassa,from
R3707	T6060	T6050	punct	.,is
R3708	T6062	T6063	nsubj	DroM,is
R3709	T6064	T6065	det	a,product
R371	T683	T681	compound	gene,family
R3710	T6065	T6063	attr	product,is
R3711	T6066	T6065	compound	gene,product
R3712	T6067	T6065	prep	from,product
R3713	T6068	T6069	compound	D.,melanogaster
R3714	T6069	T6067	pobj	melanogaster,from
R3715	T6070	T6063	punct	.,is
R3716	T6072	T6073	det	The,number
R3717	T6073	T6075	nsubj	number,is
R3718	T6074	T6073	compound	accession,number
R3719	T6076	T6073	prep	for,number
R372	T684	T675	punct	", ",cloned
R3720	T6077	T6078	det	this,gene
R3721	T6078	T6076	pobj	gene,for
R3722	T6079	T6075	attr	CG40084,is
R3723	T6080	T6075	prep	in,is
R3724	T6081	T6080	pobj	BDGP,in
R3725	T6082	T6081	punct	(,BDGP
R3726	T6083	T6084	compound	Berkeley,Project
R3727	T6084	T6081	appos	Project,BDGP
R3728	T6085	T6084	compound	Drosophila,Project
R3729	T6086	T6084	compound	Genome,Project
R373	T685	T675	nsubj	we,cloned
R3730	T6087	T6075	punct	),is
R3731	T6088	T6075	punct	.,is
R3732	T6090	T6091	nsubj	AnoG,represents
R3733	T6092	T6093	det	a,protein
R3734	T6093	T6091	dobj	protein,represents
R3735	T6094	T6093	prep	from,protein
R3736	T6095	T6096	det	the,str.
R3737	T6096	T6094	pobj	str.,from
R3738	T6097	T6096	compound	anopheles,str.
R3739	T6098	T6096	compound	gambiae,str.
R374	T686	T675	cc	and,cloned
R3740	T6099	T6100	punct	(,EAA01004
R3741	T6100	T6094	parataxis	EAA01004,from
R3742	T6101	T6100	punct	),EAA01004
R3743	T6102	T6091	punct	.,represents
R3744	T6104	T6105	nsubj	CaeE,is
R3745	T6106	T6104	punct	(,CaeE
R3746	T6107	T6104	appos	AAK77203,CaeE
R3747	T6108	T6105	punct	),is
R3748	T6109	T6110	det	a,protein
R3749	T6110	T6105	attr	protein,is
R375	T687	T675	conj	characterized,cloned
R3750	T6111	T6110	amod	hypothetical,protein
R3751	T6112	T6110	prep	from,protein
R3752	T6113	T6114	det	the,elegans
R3753	T6114	T6112	pobj	elegans,from
R3754	T6115	T6114	compound	Caenohabditis,elegans
R3755	T6116	T6105	punct	.,is
R3756	T6118	T6119	nsubj	CorC,represents
R3757	T6120	T6121	compound	bacteria,magnesium
R3758	T6121	T6119	dobj	magnesium,represents
R3759	T6122	T6121	cc	and,magnesium
R376	T688	T687	dobj	Acdp,characterized
R3760	T6123	T6124	compound	cobalt,protein
R3761	T6124	T6121	conj	protein,magnesium
R3762	T6125	T6124	compound	efflux,protein
R3763	T6126	T6121	prep	from,magnesium
R3764	T6127	T6128	det	the,oneidensis
R3765	T6128	T6126	pobj	oneidensis,from
R3766	T6129	T6128	compound	Shewanella,oneidensis
R3767	T6130	T6119	punct	.,represents
R3768	T6132	T6133	nsubj	XyFD,is
R3769	T6134	T6135	det	a,protein
R377	T689	T688	punct	", ",Acdp
R3770	T6135	T6133	attr	protein,is
R3771	T6136	T6135	amod	hypothetical,protein
R3772	T6137	T6135	prep	from,protein
R3773	T6138	T6139	det	the,Dixon
R3774	T6139	T6137	pobj	Dixon,from
R3775	T6140	T6139	compound	Xylella,Dixon
R3776	T6141	T6139	compound	fastidiosa,Dixon
R3777	T6142	T6135	punct	(,protein
R3778	T6143	T6135	appos	ZP_00038107,protein
R3779	T6144	T6133	punct	),is
R378	T690	T691	det	the,homologue
R3780	T6145	T6133	punct	.,is
R3781	T6153	T6154	amod	Phylogenetic,tree
R3782	T6155	T6154	acl	showing,tree
R3783	T6156	T6155	dobj	relationships,showing
R3784	T6157	T6156	prep	among,relationships
R3785	T6158	T6157	pobj	proteins,among
R3786	T6159	T6158	acl	containing,proteins
R3787	T6160	T6161	det	the,domain
R3788	T6161	T6159	dobj	domain,containing
R3789	T6162	T6161	compound	ACD,domain
R379	T691	T688	appos	homologue,Acdp
R3790	T6163	T6161	prep	from,domain
R3791	T6164	T6165	nmod	figure,2
R3792	T6165	T6163	pobj	2,from
R3793	T6166	T6165	cc	and,2
R3794	T6167	T6165	conj	3,2
R3795	T6168	T6154	punct	.,tree
R3796	T6170	T6171	det	The,tree
R3797	T6171	T6173	nsubjpass	tree,constructed
R3798	T6172	T6171	amod	phylogenetic,tree
R3799	T6174	T6173	auxpass	was,constructed
R380	T692	T691	compound	mouse,homologue
R3800	T6175	T6173	prep	according,constructed
R3801	T6176	T6175	prep	to,according
R3802	T6177	T6178	det	the,calculation
R3803	T6178	T6176	pobj	calculation,to
R3804	T6179	T6178	prep	of,calculation
R3805	T6180	T6181	det	the,match
R3806	T6181	T6179	pobj	match,of
R3807	T6182	T6181	amod	best,match
R3808	T6183	T6181	prep	for,match
R3809	T6184	T6185	det	the,sequences
R381	T693	T691	prep	of,homologue
R3810	T6185	T6183	pobj	sequences,for
R3811	T6186	T6185	amod	selected,sequences
R3812	T6187	T6173	punct	.,constructed
R3813	T6189	T6190	nsubj	Abbreviations,are
R3814	T6191	T6189	prep	for,Abbreviations
R3815	T6192	T6193	det	each,protein
R3816	T6193	T6191	pobj	protein,for
R3817	T6194	T6195	det	the,same
R3818	T6195	T6190	attr	same,are
R3819	T6196	T6197	mark	as,presented
R382	T694	T695	det	the,family
R3820	T6197	T6195	advcl	presented,same
R3821	T6198	T6197	prep	in,presented
R3822	T6199	T6198	pobj	figure,in
R3823	T6200	T6199	nummod	3,figure
R3824	T6201	T6190	punct	.,are
R3825	T6228	T6229	nummod	Four,domains
R3826	T6230	T6229	compound	transmembrane,domains
R3827	T6231	T6229	prep	within,domains
R3828	T6232	T6233	compound	Acdp4,protein
R3829	T6233	T6231	pobj	protein,within
R383	T695	T693	pobj	family,of
R3830	T6234	T6229	punct	.,domains
R3831	T6236	T6237	compound	Transmembrane,domains
R3832	T6237	T6238	nsubjpass	domains,predicted
R3833	T6239	T6238	auxpass	were,predicted
R3834	T6240	T6238	prep	by,predicted
R3835	T6241	T6242	det	the,program
R3836	T6242	T6240	pobj	program,by
R3837	T6243	T6242	compound	TMHMM,program
R3838	T6244	T6238	punct	.,predicted
R3839	T6246	T6247	det	The,plot
R384	T696	T695	amod	human,family
R3840	T6247	T6248	nsubj	plot,shows
R3841	T6249	T6250	det	the,probabilities
R3842	T6250	T6248	dobj	probabilities,shows
R3843	T6251	T6250	amod	posterior,probabilities
R3844	T6252	T6250	prep	of,probabilities
R3845	T6253	T6254	amod	inside,TM
R3846	T6254	T6258	compound	TM,helix
R3847	T6255	T6254	punct	/,TM
R3848	T6256	T6254	amod	outside,TM
R3849	T6257	T6254	punct	/,TM
R385	T697	T695	compound	ACDP,family
R3850	T6258	T6252	pobj	helix,of
R3851	T6259	T6248	punct	.,shows
R3852	T6261	T6262	prep	At,shown
R3853	T6263	T6264	det	the,top
R3854	T6264	T6261	pobj	top,At
R3855	T6265	T6264	prep	of,top
R3856	T6266	T6267	det	the,plot
R3857	T6267	T6265	pobj	plot,of
R3858	T6268	T6261	punct	(,At
R3859	T6269	T6261	prep	between,At
R386	T698	T695	compound	gene,family
R3860	T6270	T6269	pobj	1,between
R3861	T6271	T6270	cc	and,1
R3862	T6272	T6270	conj	1.2,1
R3863	T6273	T6262	punct	),shown
R3864	T6274	T6275	det	the,prediction
R3865	T6275	T6262	nsubjpass	prediction,shown
R3866	T6276	T6277	npadvmod	N,best
R3867	T6277	T6275	amod	best,prediction
R3868	T6278	T6277	punct	-,best
R3869	T6279	T6262	auxpass	is,shown
R387	T699	T675	punct	.,cloned
R3870	T6280	T6262	punct	.,shown
R3871	T6282	T6283	det	The,plot
R3872	T6283	T6284	nsubjpass	plot,obtained
R3873	T6285	T6284	auxpass	is,obtained
R3874	T6286	T6284	prep	by,obtained
R3875	T6287	T6286	pcomp	calculating,by
R3876	T6288	T6289	det	the,probability
R3877	T6289	T6287	dobj	probability,calculating
R3878	T6290	T6289	amod	total,probability
R3879	T6291	T6292	mark	that,sits
R388	T863	T864	amod	Molecular,cloning
R3880	T6292	T6289	acl	sits,probability
R3881	T6293	T6294	det	a,residue
R3882	T6294	T6292	nsubj	residue,sits
R3883	T6295	T6292	prep	in,sits
R3884	T6296	T6295	pobj	helix,in
R3885	T6297	T6292	punct	", ",sits
R3886	T6298	T6292	advmod	inside,sits
R3887	T6299	T6298	punct	", ",inside
R3888	T6300	T6298	cc	or,inside
R3889	T6301	T6298	conj	outside,inside
R389	T865	T864	prep	of,cloning
R3890	T6302	T6289	acl	summed,probability
R3891	T6303	T6302	prep	over,summed
R3892	T6304	T6305	det	all,paths
R3893	T6305	T6303	pobj	paths,over
R3894	T6306	T6305	amod	possible,paths
R3895	T6307	T6305	prep	through,paths
R3896	T6308	T6309	det	the,model
R3897	T6309	T6307	pobj	model,through
R3898	T6310	T6284	punct	.,obtained
R3899	T6368	T6369	dep	Fig.,analysis
R390	T866	T867	det	the,family
R3900	T6370	T6368	nummod	6A,Fig.
R3901	T6371	T6369	punct	: ,analysis
R3902	T6372	T6369	compound	Immunoblot,analysis
R3903	T6373	T6369	prep	of,analysis
R3904	T6374	T6375	compound	Acdp,proteins
R3905	T6375	T6373	pobj	proteins,of
R3906	T6376	T6375	prep	in,proteins
R3907	T6377	T6378	compound	brain,tissue
R3908	T6378	T6379	compound	tissue,extracts
R3909	T6379	T6376	pobj	extracts,in
R391	T867	T865	pobj	family,of
R3910	T6380	T6369	punct	.,analysis
R3911	T6382	T6383	nsubjpass	Immunoblotting,carried
R3912	T6384	T6383	auxpass	were,carried
R3913	T6385	T6383	prt	out,carried
R3914	T6386	T6383	advcl	using,carried
R3915	T6387	T6388	det	a,system
R3916	T6388	T6386	dobj	system,using
R3917	T6389	T6390	compound	Western,blotting
R3918	T6390	T6388	compound	blotting,system
R3919	T6391	T6388	compound	detection,system
R392	T868	T867	compound	Acdp,family
R3920	T6392	T6393	punct	(,ECL
R3921	T6393	T6386	parataxis	ECL,using
R3922	T6394	T6393	punct	),ECL
R3923	T6395	T6396	punct	(,PIERCE
R3924	T6396	T6386	parataxis	PIERCE,using
R3925	T6397	T6396	punct	),PIERCE
R3926	T6398	T6383	punct	.,carried
R3927	T6400	T6401	nsubj	Lane,probed
R3928	T6402	T6400	nummod	1,Lane
R3929	T6403	T6401	punct	", ",probed
R393	T869	T867	compound	gene,family
R3930	T6404	T6401	prep	with,probed
R3931	T6405	T6404	pobj	antibody,with
R3932	T6406	T6405	acl	generated,antibody
R3933	T6407	T6406	agent	by,generated
R3934	T6408	T6409	amod	conserved,peptide
R3935	T6409	T6407	pobj	peptide,by
R3936	T6410	T6401	punct	.,probed
R3937	T6412	T6413	prep	From,band
R3938	T6414	T6412	pobj	top,From
R3939	T6415	T6412	prep	to,From
R394	T871	T872	aux	To,clone
R3940	T6416	T6415	pobj	bottom,to
R3941	T6417	T6413	punct	", ",band
R3942	T6418	T6413	det	each,band
R3943	T6419	T6413	acl	corresponding,band
R3944	T6420	T6419	prep	to,corresponding
R3945	T6421	T6420	pobj	Acdp1,to
R3946	T6422	T6423	punct	(,kD
R3947	T6423	T6421	parataxis	kD,Acdp1
R3948	T6424	T6423	nummod	115,kD
R3949	T6425	T6423	punct	),kD
R395	T872	T873	advcl	clone,used
R3950	T6426	T6421	punct	", ",Acdp1
R3951	T6427	T6421	conj	Acdp2,Acdp1
R3952	T6428	T6429	punct	(,kD
R3953	T6429	T6427	parataxis	kD,Acdp2
R3954	T6430	T6429	nummod	100,kD
R3955	T6431	T6429	punct	),kD
R3956	T6432	T6427	punct	", ",Acdp2
R3957	T6433	T6427	conj	Acdp4,Acdp2
R3958	T6434	T6435	punct	(,kD
R3959	T6435	T6433	parataxis	kD,Acdp4
R396	T874	T875	det	the,genes
R3960	T6436	T6435	nummod	90,kD
R3961	T6437	T6435	punct	),kD
R3962	T6438	T6433	cc	and,Acdp4
R3963	T6439	T6433	conj	Acdp3,Acdp4
R3964	T6440	T6441	punct	(,kD
R3965	T6441	T6439	parataxis	kD,Acdp3
R3966	T6442	T6441	nummod	80,kD
R3967	T6443	T6441	punct	),kD
R3968	T6444	T6413	punct	.,band
R3969	T6446	T6447	nmod	Lane,2
R397	T875	T872	dobj	genes,clone
R3970	T6447	T6448	nsubj	2,probed
R3971	T6449	T6447	cc	and,2
R3972	T6450	T6447	conj	3,2
R3973	T6451	T6448	punct	", ",probed
R3974	T6452	T6448	prep	with,probed
R3975	T6453	T6454	det	the,antibodies
R3976	T6454	T6452	pobj	antibodies,with
R3977	T6455	T6454	compound	Acdp1,antibodies
R3978	T6456	T6454	acl	generated,antibodies
R3979	T6457	T6456	agent	by,generated
R398	T876	T875	compound	mouse,genes
R3980	T6458	T6459	npadvmod	N,terminal
R3981	T6459	T6461	amod	terminal,peptides
R3982	T6460	T6459	punct	-,terminal
R3983	T6461	T6457	pobj	peptides,by
R3984	T6462	T6459	cc	and,terminal
R3985	T6463	T6464	npadvmod	C,terminal
R3986	T6464	T6459	conj	terminal,terminal
R3987	T6465	T6464	punct	-,terminal
R3988	T6466	T6456	punct	", ",generated
R3989	T6467	T6456	advmod	respectively,generated
R399	T877	T875	compound	Acdp,genes
R3990	T6468	T6448	punct	.,probed
R3991	T6470	T6471	dep	Fig.,analysis
R3992	T6472	T6470	nummod	6B,Fig.
R3993	T6473	T6471	punct	: ,analysis
R3994	T6474	T6471	compound	Immunoblot,analysis
R3995	T6475	T6471	prep	of,analysis
R3996	T6476	T6475	pobj	Acdp1,of
R3997	T6477	T6471	prep	in,analysis
R3998	T6478	T6479	nmod	HEK293,cells
R3999	T6479	T6477	pobj	cells,in
R400	T878	T873	punct	", ",used
R4000	T6480	T6478	punct	", ",HEK293
R4001	T6481	T6478	conj	3T3,HEK293
R4002	T6482	T6481	cc	and,3T3
R4003	T6483	T6481	conj	PC12,3T3
R4004	T6484	T6471	punct	.,analysis
R4005	T6486	T6487	det	The,blots
R4006	T6487	T6488	nsubjpass	blots,probed
R4007	T6489	T6488	auxpass	were,probed
R4008	T6490	T6488	prep	with,probed
R4009	T6491	T6492	det	the,antibody
R401	T879	T880	det	the,cDNA
R4010	T6492	T6490	pobj	antibody,with
R4011	T6493	T6492	prep	against,antibody
R4012	T6494	T6495	det	the,terminus
R4013	T6495	T6493	pobj	terminus,against
R4014	T6496	T6495	compound	C,terminus
R4015	T6497	T6495	punct	-,terminus
R4016	T6498	T6495	prep	of,terminus
R4017	T6499	T6498	pobj	Acdp,of
R4018	T6500	T6499	punct	-,Acdp
R4019	T6501	T6499	nummod	1,Acdp
R402	T880	T873	nsubjpass	cDNA,used
R4020	T6502	T6488	punct	.,probed
R4021	T6504	T6505	dep	Lane,μg
R4022	T6506	T6504	nummod	1,Lane
R4023	T6507	T6505	punct	", ",μg
R4024	T6508	T6505	nummod	10,μg
R4025	T6509	T6505	prep	of,μg
R4026	T6510	T6511	compound	HEK293,lysates
R4027	T6511	T6509	pobj	lysates,of
R4028	T6512	T6511	compound	cell,lysates
R4029	T6513	T6505	punct	.,μg
R403	T881	T880	amod	human,cDNA
R4030	T6515	T6516	nmod	Lane,2
R4031	T6516	T6517	dep	2,μg
R4032	T6518	T6516	cc	and,2
R4033	T6519	T6516	conj	3,2
R4034	T6520	T6517	punct	", ",μg
R4035	T6521	T6517	nummod	100,μg
R4036	T6522	T6517	prep	of,μg
R4037	T6523	T6524	nmod	3T3,lysates
R4038	T6524	T6522	pobj	lysates,of
R4039	T6525	T6523	cc	and,3T3
R404	T882	T880	compound	ACDP,cDNA
R4040	T6526	T6523	conj	PC12,3T3
R4041	T6527	T6524	compound	cell,lysates
R4042	T6528	T6517	punct	.,μg
R4043	T6553	T6554	amod	Subcellular,localization
R4044	T6555	T6554	prep	of,localization
R4045	T6556	T6555	pobj	Acdp1,of
R4046	T6557	T6554	prep	in,localization
R4047	T6558	T6559	compound	hippocampus,neurons
R4048	T6559	T6557	pobj	neurons,in
R4049	T6560	T6554	punct	.,localization
R405	T883	T880	cc	and,cDNA
R4050	T6562	T6563	det	A,series
R4051	T6564	T6563	prep	of,series
R4052	T6565	T6566	amod	confocal,images
R4053	T6566	T6564	pobj	images,of
R4054	T6567	T6563	prep	from,series
R4055	T6568	T6569	det	a,neuron
R4056	T6569	T6567	pobj	neuron,from
R4057	T6570	T6569	amod	cultured,neuron
R4058	T6571	T6569	acl	stained,neuron
R4059	T6572	T6571	prep	with,stained
R406	T884	T885	amod	predicted,sequences
R4060	T6573	T6574	det	an,antibody
R4061	T6574	T6572	pobj	antibody,with
R4062	T6575	T6574	amod	anti-Acdp1,antibody
R4063	T6576	T6563	punct	.,series
R4064	T6578	T6579	det	The,step
R4065	T6579	T6580	nsubj	step,is
R4066	T6581	T6579	prep	of,step
R4067	T6582	T6583	det	each,section
R4068	T6583	T6581	pobj	section,of
R4069	T6584	T6583	compound	imaging,section
R407	T885	T880	conj	sequences,cDNA
R4070	T6585	T6586	nummod	0.5,μm
R4071	T6586	T6580	attr	μm,is
R4072	T6587	T6580	punct	", ",is
R4073	T6588	T6580	prep	from,is
R4074	T6589	T6590	det	the,surface
R4075	T6590	T6588	pobj	surface,from
R4076	T6591	T6590	prep	of,surface
R4077	T6592	T6593	det	the,neuron
R4078	T6593	T6591	pobj	neuron,of
R4079	T6594	T6595	punct	(,μm
R408	T886	T885	compound	protein,sequences
R4080	T6595	T6590	parataxis	μm,surface
R4081	T6596	T6595	nummod	0,μm
R4082	T6597	T6595	punct	),μm
R4083	T6598	T6588	prep	to,from
R4084	T6599	T6600	det	the,plan
R4085	T6600	T6598	pobj	plan,to
R4086	T6601	T6600	amod	middle,plan
R4087	T6602	T6603	punct	(,μm
R4088	T6603	T6600	parataxis	μm,plan
R4089	T6604	T6603	nummod	4.5,μm
R409	T887	T873	auxpass	were,used
R4090	T6605	T6603	punct	),μm
R4091	T6606	T6580	punct	.,is
R4092	T6623	T6624	nmod	Nucleotide,homologies
R4093	T6625	T6623	cc	and,Nucleotide
R4094	T6626	T6627	compound	amino,acid
R4095	T6627	T6623	conj	acid,Nucleotide
R4096	T6628	T6629	punct	(,%
R4097	T6629	T6624	parataxis	%,homologies
R4098	T6630	T6629	punct	),%
R4099	T6631	T6624	prep	between,homologies
R410	T888	T889	aux	to,search
R4100	T6632	T6633	amod	human,ACDP
R4101	T6633	T6634	nmod	ACDP,members
R4102	T6634	T6631	pobj	members,between
R4103	T6635	T6633	cc	and,ACDP
R4104	T6636	T6637	compound	mouse,Acdp
R4105	T6637	T6633	conj	Acdp,ACDP
R4106	T6638	T6624	punct	.,homologies
R411	T889	T873	advcl	search,used
R412	T890	T891	det	the,database
R413	T891	T889	dobj	database,search
R414	T892	T891	compound	mouse,database
R415	T893	T891	compound	EST,database
R416	T894	T889	prep	with,search
R417	T895	T896	det	the,programs
R418	T896	T894	pobj	programs,with
R419	T897	T896	nmod	blastn,programs
R420	T898	T897	cc	and,blastn
R421	T899	T897	conj	tblastn,blastn
R422	T900	T873	punct	.,used
R423	T902	T903	compound	Mouse,markers
R424	T903	T905	nsubjpass	markers,identified
R425	T904	T903	compound	EST,markers
R426	T906	T903	acl	corresponding,markers
R427	T907	T906	prep	to,corresponding
R428	T908	T909	det	each,member
R429	T909	T907	pobj	member,to
R430	T910	T909	compound	Acdp,member
R431	T911	T905	auxpass	were,identified
R432	T912	T905	advmod	then,identified
R433	T913	T905	punct	.,identified
R434	T915	T916	prep	For,corresponds
R435	T917	T915	pobj	example,For
R436	T918	T916	punct	", ",corresponds
R437	T919	T920	compound	EST,H3086H12
R438	T920	T916	nsubj	H3086H12,corresponds
R439	T921	T920	punct	-,H3086H12
R440	T922	T920	nummod	5,H3086H12
R441	T923	T916	prep	to,corresponds
R442	T924	T923	pobj	Acdp1,to
R443	T925	T916	punct	", ",corresponds
R444	T926	T916	conj	W98010,corresponds
R445	T927	T926	prep	for,W98010
R446	T928	T927	pobj	Acdp2,for
R447	T929	T926	punct	", ",W98010
R448	T930	T926	conj	603299135F1,W98010
R449	T931	T930	prep	for,603299135F1
R450	T932	T931	pobj	Acdp3,for
R451	T933	T930	cc	and,603299135F1
R452	T934	T930	conj	BG083791,603299135F1
R453	T935	T934	prep	for,BG083791
R454	T936	T935	pobj	Acdp4,for
R455	T937	T916	punct	.,corresponds
R456	T939	T940	det	A,dT
R457	T940	T944	nsubjpass	dT,used
R458	T941	T940	amod	modified,dT
R459	T942	T940	compound	oligo,dT
R460	T943	T940	punct	-,dT
R461	T945	T940	prep	with,dT
R462	T946	T947	det	a,tail
R463	T947	T945	pobj	tail,with
R464	T948	T947	compound	M13,tail
R465	T949	T944	auxpass	was,used
R466	T950	T944	prep	for,used
R467	T951	T952	det	the,reaction
R468	T952	T950	pobj	reaction,for
R469	T953	T952	compound	RT,reaction
R470	T954	T944	punct	.,used
R471	T956	T957	det	A,primer
R472	T957	T959	nsubjpass	primer,used
R473	T958	T957	amod	forward,primer
R474	T960	T957	prep	from,primer
R475	T961	T962	det	each,marker
R476	T962	T960	pobj	marker,from
R477	T963	T962	compound	EST,marker
R478	T964	T962	cc	and,marker
R479	T965	T966	det	the,primer
R480	T966	T962	conj	primer,marker
R481	T967	T966	compound	M13,primer
R482	T968	T966	punct	(,primer
R483	T969	T970	compound	olig,dT
R484	T970	T972	compound	dT,tail
R485	T971	T970	punct	-,dT
R486	T972	T966	appos	tail,primer
R487	T973	T966	punct	),primer
R488	T974	T959	auxpass	were,used
R489	T975	T976	aux	to,amplify
R490	T976	T959	advcl	amplify,used
R491	T977	T978	det	the,sequence
R492	T978	T976	dobj	sequence,amplify
R493	T979	T980	nummod	3,UTR
R494	T980	T978	compound	UTR,sequence
R495	T981	T979	punct	',3
R496	T982	T976	prep	for,amplify
R497	T983	T984	det	each,gene
R498	T984	T982	pobj	gene,for
R499	T985	T984	amod	corresponding,gene
R500	T986	T984	compound	Acdp,gene
R501	T987	T984	prep	from,gene
R502	T988	T989	det	the,products
R503	T989	T987	pobj	products,from
R504	T990	T989	compound	RT,products
R505	T991	T959	punct	.,used
R506	T993	T994	aux	To,obtain
R507	T994	T995	advcl	obtain,conducted
R508	T996	T997	nummod	5,end
R509	T997	T1000	nmod	end,sequences
R510	T998	T996	punct	',5
R511	T999	T997	punct	-,end
R512	T1000	T994	dobj	sequences,obtain
R513	T1001	T1000	amod	coding,sequences
R514	T1002	T994	prep	for,obtain
R515	T1003	T1004	det	the,genes
R516	T1004	T1002	pobj	genes,for
R517	T1005	T1004	compound	Acdp,genes
R518	T1006	T995	punct	", ",conducted
R519	T1007	T995	nsubj	we,conducted
R520	T1008	T1009	det	a,PCR
R521	T1009	T995	dobj	PCR,conducted
R522	T1010	T1011	npadvmod	series,nested
R523	T1011	T1009	amod	nested,PCR
R524	T1012	T995	prep	with,conducted
R525	T1013	T1012	pobj	combinations,with
R526	T1014	T1013	prep	of,combinations
R527	T1015	T1016	nmod	mouse,primers
R528	T1016	T1014	pobj	primers,of
R529	T1017	T1015	cc	and,mouse
R530	T1018	T1015	conj	human,mouse
R531	T1019	T995	punct	.,conducted
R532	T1021	T1022	det	The,sequences
R533	T1022	T1026	nsubjpass	sequences,identified
R534	T1023	T1022	nummod	5,sequences
R535	T1024	T1023	punct	',5
R536	T1025	T1022	compound	UTR,sequences
R537	T1027	T1026	auxpass	were,identified
R538	T1028	T1026	prep	by,identified
R539	T1029	T1030	advmod	directly,sequencing
R540	T1030	T1028	pcomp	sequencing,by
R541	T1031	T1032	compound	BAC,DNA
R542	T1032	T1030	dobj	DNA,sequencing
R543	T1033	T1032	acl	containing,DNA
R544	T1034	T1035	det	the,genes
R545	T1035	T1033	dobj	genes,containing
R546	T1036	T1035	amod	corresponding,genes
R547	T1037	T1035	compound	Acdp,genes
R548	T1038	T1026	punct	.,identified
R549	T1040	T1041	det	The,clones
R550	T1041	T1043	nsubjpass	clones,identified
R551	T1042	T1041	compound	BAC,clones
R552	T1044	T1043	auxpass	were,identified
R553	T1045	T1043	prep	by,identified
R554	T1046	T1045	pcomp	screening,by
R555	T1047	T1048	det	a,library
R556	T1048	T1046	dobj	library,screening
R557	T1049	T1050	compound	CITB,DNA
R558	T1050	T1048	compound	DNA,library
R559	T1051	T1050	compound	mouse,DNA
R560	T1052	T1050	compound	BAC,DNA
R561	T1053	T1054	punct	(,Genetics
R562	T1054	T1048	parataxis	Genetics,library
R563	T1055	T1054	compound	Research,Genetics
R564	T1056	T1054	punct	),Genetics
R565	T1057	T1043	punct	.,identified
R566	T1059	T1060	det	The,sequences
R567	T1060	T1064	nsubjpass	sequences,confirmed
R568	T1061	T1060	nummod	5,sequences
R569	T1062	T1061	punct	',5
R570	T1063	T1060	compound	UTR,sequences
R571	T1065	T1060	acl	obtained,sequences
R572	T1066	T1065	prep	from,obtained
R573	T1067	T1066	pcomp	above,from
R574	T1068	T1064	auxpass	were,confirmed
R575	T1069	T1064	advmod	further,confirmed
R576	T1070	T1064	prep	by,confirmed
R577	T1071	T1072	compound	RT,PCR
R578	T1072	T1070	pobj	PCR,by
R579	T1073	T1072	punct	-,PCR
R580	T1074	T1064	punct	.,confirmed
R581	T1076	T1077	det	The,gene
R582	T1077	T1079	nsubj	gene,contains
R583	T1078	T1077	compound	Acdp1,gene
R584	T1080	T1081	nummod	"3,631",bp
R585	T1081	T1079	dobj	bp,contains
R586	T1082	T1081	prep	of,bp
R587	T1083	T1084	compound	nucleotide,sequence
R588	T1084	T1082	pobj	sequence,of
R589	T1085	T1079	cc	and,contains
R590	T1086	T1079	conj	encodes,contains
R591	T1087	T1088	det	a,protein
R592	T1088	T1086	dobj	protein,encodes
R593	T1089	T1088	amod	predicted,protein
R594	T1090	T1086	prep	with,encodes
R595	T1091	T1092	nummod	951,acids
R596	T1092	T1090	pobj	acids,with
R597	T1093	T1092	compound	amino,acids
R598	T1094	T1095	punct	(,AA
R599	T1095	T1086	parataxis	AA,encodes
R600	T1096	T1095	punct	),AA
R601	T1097	T1079	punct	.,contains
R602	T1099	T1100	det	The,genes
R603	T1100	T1104	nsubj	genes,contain
R604	T1101	T1100	amod	other,genes
R605	T1102	T1100	nummod	three,genes
R606	T1103	T1100	compound	Acdp,genes
R607	T1105	T1100	punct	(,genes
R608	T1106	T1100	appos	Acdp2,genes
R609	T1107	T1106	punct	", ",Acdp2
R610	T1108	T1106	nummod	3,Acdp2
R611	T1109	T1106	cc	and,Acdp2
R612	T1110	T1106	conj	4,Acdp2
R613	T1111	T1100	punct	),genes
R614	T1112	T1113	nummod	"3,244",bp
R615	T1113	T1104	dobj	bp,contain
R616	T1114	T1113	punct	", ",bp
R617	T1115	T1116	nummod	"2,684",bp
R618	T1116	T1113	conj	bp,bp
R619	T1117	T1116	cc	and,bp
R620	T1118	T1119	nummod	"2,743",bp
R621	T1119	T1116	conj	bp,bp
R622	T1120	T1113	prep	of,bp
R623	T1121	T1122	compound	cDNA,sequences
R624	T1122	T1120	pobj	sequences,of
R625	T1123	T1104	punct	", ",contain
R626	T1124	T1104	cc	and,contain
R627	T1125	T1104	conj	encode,contain
R628	T1126	T1127	amod	deduced,proteins
R629	T1127	T1125	dobj	proteins,encode
R630	T1128	T1127	prep	of,proteins
R631	T1129	T1130	nummod	874,acids
R632	T1130	T1128	pobj	acids,of
R633	T1131	T1130	compound	amino,acids
R634	T1132	T1130	punct	", ",acids
R635	T1133	T1134	nummod	713,acids
R636	T1134	T1130	conj	acids,acids
R637	T1135	T1134	compound	amino,acids
R638	T1136	T1134	cc	and,acids
R639	T1137	T1138	nummod	771,acids
R640	T1138	T1134	conj	acids,acids
R641	T1139	T1138	compound	amino,acids
R642	T1140	T1125	punct	", ",encode
R643	T1141	T1125	advmod	respectively,encode
R644	T1142	T1104	punct	.,contain
R645	T1296	T1297	compound	Tissue,distribution
R646	T1299	T1300	compound	Northern,blot
R647	T1300	T1301	compound	blot,analyses
R648	T1301	T1302	nsubjpass	analyses,carried
R649	T1303	T1301	prep	of,analyses
R650	T1304	T1305	det	the,family
R651	T1305	T1303	pobj	family,of
R652	T1306	T1305	compound	Acdp,family
R653	T1307	T1305	compound	gene,family
R654	T1308	T1302	auxpass	were,carried
R655	T1309	T1302	prt	out,carried
R656	T1310	T1302	advcl	using,carried
R657	T1311	T1310	dobj	membranes,using
R658	T1312	T1311	acl	purchased,membranes
R659	T1313	T1312	prep	from,purchased
R660	T1314	T1313	pobj	Origene,from
R661	T1315	T1302	punct	.,carried
R662	T1317	T1318	det	A,total
R663	T1318	T1319	nsubjpass	total,included
R664	T1320	T1318	prep	of,total
R665	T1321	T1322	nummod	12,tissues
R666	T1322	T1320	pobj	tissues,of
R667	T1323	T1322	compound	mouse,tissues
R668	T1324	T1319	auxpass	were,included
R669	T1325	T1319	prep	in,included
R670	T1326	T1327	det	the,study
R671	T1327	T1325	pobj	study,in
R672	T1328	T1329	punct	(,Fig.
R673	T1329	T1319	parataxis	Fig.,included
R674	T1330	T1329	nummod	1,Fig.
R675	T1331	T1329	punct	),Fig.
R676	T1332	T1319	punct	.,included
R677	T1334	T1335	prep	Due,were
R678	T1336	T1334	pcomp	to,Due
R679	T1337	T1338	compound	sequence,homologies
R680	T1338	T1334	pobj	homologies,Due
R681	T1339	T1338	prep	between,homologies
R682	T1340	T1341	det	each,member
R683	T1341	T1339	pobj	member,between
R684	T1342	T1341	compound	Acdp,member
R685	T1343	T1341	prep	within,member
R686	T1344	T1345	det	the,domain
R687	T1345	T1343	pobj	domain,within
R688	T1346	T1345	amod	conserved,domain
R689	T1347	T1335	punct	", ",were
R690	T1348	T1335	nsubj	probes,were
R691	T1349	T1348	prep	for,probes
R692	T1350	T1351	compound	Northern,bolts
R693	T1351	T1349	pobj	bolts,for
R694	T1352	T1353	compound	PCR,fragments
R695	T1353	T1335	attr	fragments,were
R696	T1354	T1353	prep	from,fragments
R697	T1355	T1356	det	the,exon
R698	T1356	T1354	pobj	exon,from
R699	T1357	T1356	amod	last,exon
R700	T1358	T1356	cc	and,exon
R701	T1359	T1360	det	the,sequences
R702	T1360	T1356	conj	sequences,exon
R703	T1361	T1362	nummod	3,region
R704	T1362	T1360	compound	region,sequences
R705	T1363	T1361	punct	',3
R706	T1364	T1362	amod	untranslated,region
R707	T1365	T1335	punct	.,were
R708	T1367	T1368	det	The,messages
R709	T1368	T1371	nsubj	messages,showed
R710	T1369	T1368	compound	mouse,messages
R711	T1370	T1368	compound	Acdp,messages
R712	T1372	T1373	advmod	almost,distributions
R713	T1373	T1371	dobj	distributions,showed
R714	T1374	T1373	det	the,distributions
R715	T1375	T1373	amod	same,distributions
R716	T1376	T1373	compound	tissue,distributions
R717	T1377	T1373	prep	as,distributions
R718	T1378	T1379	det	the,genes
R719	T1379	T1377	pobj	genes,as
R720	T1380	T1379	amod	human,genes
R721	T1381	T1379	compound	ACDP,genes
R722	T1382	T1371	punct	.,showed
R723	T1384	T1385	compound	Acdp1,message
R724	T1385	T1386	nsubjpass	message,expressed
R725	T1387	T1386	auxpass	is,expressed
R726	T1388	T1386	advmod	highly,expressed
R727	T1389	T1386	prep	in,expressed
R728	T1390	T1391	det	the,brain
R729	T1391	T1389	pobj	brain,in
R730	T1392	T1386	punct	", ",expressed
R731	T1393	T1394	mark	while,showed
R732	T1394	T1386	advcl	showed,expressed
R733	T1395	T1394	nsubj	kidney,showed
R734	T1396	T1395	cc	and,kidney
R735	T1397	T1395	conj	testis,kidney
R736	T1398	T1394	advmod	also,showed
R737	T1399	T1400	amod	low,levels
R738	T1400	T1394	dobj	levels,showed
R739	T1401	T1400	prep	of,levels
R740	T1402	T1401	pobj	expression,of
R741	T1403	T1386	punct	.,expressed
R742	T1405	T1406	nsubj	Acdp2,showed
R743	T1407	T1408	amod	higher,expressions
R744	T1408	T1406	dobj	expressions,showed
R745	T1409	T1406	prep	in,showed
R746	T1410	T1411	det	the,brain
R747	T1411	T1409	pobj	brain,in
R748	T1412	T1411	punct	", ",brain
R749	T1413	T1411	conj	kidney,brain
R750	T1414	T1413	cc	and,kidney
R751	T1415	T1413	conj	liver,kidney
R752	T1416	T1406	punct	.,showed
R753	T1418	T1419	advmod	However,was
R754	T1420	T1419	punct	", ",was
R755	T1421	T1422	det	the,transcript
R756	T1422	T1419	nsubj	transcript,was
R757	T1423	T1422	compound	Acdp2,transcript
R758	T1424	T1419	neg	not,was
R759	T1425	T1419	acomp	present,was
R760	T1426	T1419	prep	in,was
R761	T1427	T1428	det	the,muscle
R762	T1428	T1426	pobj	muscle,in
R763	T1429	T1428	compound	skeleton,muscle
R764	T1430	T1428	cc	and,muscle
R765	T1431	T1428	conj	skin,muscle
R766	T1432	T1419	punct	", ",was
R767	T1433	T1419	cc	and,was
R768	T1434	T1435	nsubj	it,showed
R769	T1435	T1419	conj	showed,was
R770	T1436	T1437	advmod	very,low
R771	T1437	T1438	amod	low,levels
R772	T1438	T1435	dobj	levels,showed
R773	T1439	T1438	prep	of,levels
R774	T1440	T1439	pobj	expression,of
R775	T1441	T1435	prep	in,showed
R776	T1442	T1443	det	the,rest
R777	T1443	T1441	pobj	rest,in
R778	T1444	T1443	prep	of,rest
R779	T1445	T1444	pobj	tissues,of
R780	T1446	T1435	punct	.,showed
R781	T1448	T1449	nsubj	Acdp3,showed
R782	T1449	T1452	ccomp	showed,observed
R783	T1450	T1448	cc	and,Acdp3
R784	T1451	T1448	conj	Acdp4,Acdp3
R785	T1453	T1454	amod	different,levels
R786	T1454	T1449	dobj	levels,showed
R787	T1455	T1454	prep	of,levels
R788	T1456	T1455	pobj	expression,of
R789	T1457	T1449	prep	in,showed
R790	T1458	T1459	det	all,tissues
R791	T1459	T1457	pobj	tissues,in
R792	T1460	T1459	acl	tested,tissues
R793	T1461	T1452	punct	;,observed
R794	T1462	T1463	det	the,expressions
R795	T1463	T1452	nsubjpass	expressions,observed
R796	T1464	T1463	amod	highest,expressions
R797	T1465	T1463	prep	for,expressions
R798	T1466	T1465	pobj	Acdp3,for
R799	T1467	T1452	auxpass	were,observed
R800	T1468	T1452	prep	in,observed
R801	T1469	T1470	det	the,brain
R802	T1470	T1468	pobj	brain,in
R803	T1471	T1470	punct	", ",brain
R804	T1472	T1470	conj	kidney,brain
R805	T1473	T1472	punct	", ",kidney
R806	T1474	T1472	conj	liver,kidney
R807	T1475	T1474	cc	and,liver
R808	T1476	T1474	conj	heart,liver
R809	T1477	T1452	punct	", ",observed
R810	T1478	T1452	cc	and,observed
R811	T1479	T1480	det	the,expressions
R812	T1480	T1482	nsubjpass	expressions,observed
R813	T1481	T1480	amod	highest,expressions
R814	T1482	T1452	conj	observed,observed
R815	T1483	T1480	prep	for,expressions
R816	T1484	T1483	pobj	Acdp4,for
R817	T1485	T1482	auxpass	were,observed
R818	T1486	T1482	prep	in,observed
R819	T1487	T1488	det	the,kidney
R820	T1488	T1486	pobj	kidney,in
R821	T1489	T1488	punct	", ",kidney
R822	T1490	T1491	amod	small,intestine
R823	T1491	T1488	conj	intestine,kidney
R824	T1492	T1491	cc	and,intestine
R825	T1493	T1491	conj	testis,intestine
R826	T1494	T1452	punct	.,observed
R827	T1496	T1497	det	The,levels
R828	T1497	T1499	nsubj	levels,were
R829	T1498	T1497	compound	expression,levels
R830	T1499	T1507	ccomp	were,showed
R831	T1500	T1497	prep	for,levels
R832	T1501	T1500	pobj	Acdp3,for
R833	T1502	T1501	cc	and,Acdp3
R834	T1503	T1501	conj	4,Acdp3
R835	T1504	T1497	prep	in,levels
R836	T1505	T1506	compound	skeleton,muscle
R837	T1506	T1504	pobj	muscle,in
R838	T1508	T1509	advmod	barely,detectable
R839	T1509	T1499	acomp	detectable,were
R840	T1510	T1507	punct	;,showed
R841	T1511	T1507	advmod	however,showed
R842	T1512	T1507	punct	", ",showed
R843	T1513	T1514	compound	β,actin
R844	T1514	T1507	nsubj	actin,showed
R845	T1515	T1514	punct	-,actin
R846	T1516	T1517	amod	normal,expression
R847	T1517	T1507	dobj	expression,showed
R848	T1518	T1507	advcl	suggesting,showed
R849	T1519	T1520	mark	that,were
R850	T1520	T1518	ccomp	were,suggesting
R851	T1521	T1522	det	the,results
R852	T1522	T1520	nsubj	results,were
R853	T1523	T1520	neg	not,were
R854	T1524	T1525	det	a,consequence
R855	T1525	T1520	attr	consequence,were
R856	T1526	T1525	prep	of,consequence
R857	T1527	T1528	amod	bad,quality
R858	T1528	T1526	pobj	quality,of
R859	T1529	T1528	compound	RNA,quality
R860	T1530	T1531	punct	(,shown
R861	T1531	T1507	parataxis	shown,showed
R862	T1532	T1531	nsubj	data,shown
R863	T1533	T1531	neg	not,shown
R864	T1534	T1531	punct	),shown
R865	T1535	T1507	punct	.,showed
R866	T1537	T1538	det	The,pattern
R867	T1538	T1541	nsubjpass	pattern,taken
R868	T1539	T1538	amod	ubiquitous,pattern
R869	T1540	T1538	compound	expression,pattern
R870	T1542	T1541	aux	may,taken
R871	T1543	T1541	auxpass	be,taken
R872	T1544	T1541	prep	as,taken
R873	T1545	T1546	det	another,indication
R874	T1546	T1544	pobj	indication,as
R875	T1547	T1546	prep	of,indication
R876	T1548	T1549	det	the,importance
R877	T1549	T1547	pobj	importance,of
R878	T1550	T1549	amod	functional,importance
R879	T1551	T1549	prep	of,importance
R880	T1552	T1553	compound	Acdp,proteins
R881	T1553	T1551	pobj	proteins,of
R882	T1554	T1549	prep	in,importance
R883	T1555	T1556	amod	fundamental,processes
R884	T1556	T1554	pobj	processes,in
R885	T1557	T1556	amod	biological,processes
R886	T1558	T1541	prep	in,taken
R887	T1559	T1558	pobj	addition,in
R888	T1560	T1559	prep	to,addition
R889	T1561	T1562	det	the,conservation
R890	T1562	T1560	pobj	conservation,to
R891	T1563	T1562	compound	sequence,conservation
R892	T1564	T1562	prep	in,conservation
R893	T1565	T1566	advmod	evolutionarily,divergent
R894	T1566	T1567	amod	divergent,species
R895	T1567	T1564	pobj	species,in
R896	T1568	T1541	punct	.,taken
R897	T1609	T1610	amod	Chromosomal,location
R898	T1612	T1613	compound	Radiation,mapping
R899	T1613	T1615	nsubj	mapping,indicated
R900	T1614	T1613	compound	hybrid,mapping
R901	T1616	T1617	mark	that,maps
R902	T1617	T1615	ccomp	maps,indicated
R903	T1618	T1619	det	the,gene
R904	T1619	T1617	nsubj	gene,maps
R905	T1620	T1619	compound	Acdp1,gene
R906	T1621	T1617	prep	to,maps
R907	T1622	T1621	pobj	chromosome,to
R908	T1623	T1622	nummod	19,chromosome
R909	T1624	T1617	prep	between,maps
R910	T1625	T1626	compound	markers,D19Mit119
R911	T1626	T1624	pobj	D19Mit119,between
R912	T1627	T1628	punct	(,cR
R913	T1628	T1626	parataxis	cR,D19Mit119
R914	T1629	T1628	nummod	34.3,cR
R915	T1630	T1628	amod	proximal,cR
R916	T1631	T1628	punct	),cR
R917	T1632	T1626	cc	and,D19Mit119
R918	T1633	T1626	conj	D19Mit112,D19Mit119
R919	T1634	T1635	punct	(,cR
R920	T1635	T1633	parataxis	cR,D19Mit112
R921	T1636	T1635	nummod	13.6,cR
R922	T1637	T1635	amod	distal,cR
R923	T1638	T1635	punct	),cR
R924	T1639	T1615	punct	.,indicated
R925	T1641	T1642	det	The,gene
R926	T1642	T1644	nsubj	gene,maps
R927	T1643	T1642	compound	Acdp2,gene
R928	T1645	T1646	advmod	slightly,more
R929	T1646	T1647	advmod	more,distal
R930	T1647	T1644	advcl	distal,maps
R931	T1648	T1644	prep	to,maps
R932	T1649	T1650	det	the,Acdp1
R933	T1650	T1648	pobj	Acdp1,to
R934	T1651	T1650	prep	on,Acdp1
R935	T1652	T1651	pobj	chromosome,on
R936	T1653	T1652	nummod	19,chromosome
R937	T1654	T1644	prep	between,maps
R938	T1655	T1654	pobj	D19Mit9,between
R939	T1656	T1657	punct	(,cR
R940	T1657	T1655	parataxis	cR,D19Mit9
R941	T1658	T1657	nummod	2.4,cR
R942	T1659	T1657	amod	proximal,cR
R943	T1660	T1657	punct	),cR
R944	T1661	T1655	cc	and,D19Mit9
R945	T1662	T1655	conj	D19Mit38,D19Mit9
R946	T1663	T1664	punct	(,cR
R947	T1664	T1662	parataxis	cR,D19Mit38
R948	T1665	T1664	nummod	15.1,cR
R949	T1666	T1664	amod	distal,cR
R950	T1667	T1664	punct	),cR
R951	T1668	T1644	punct	.,maps
R952	T1670	T1671	det	The,genes
R953	T1671	T1675	nsubj	genes,map
R954	T1672	T1671	nmod	Acdp3,genes
R955	T1673	T1672	cc	and,Acdp3
R956	T1674	T1672	conj	Acdp4,Acdp3
R957	T1676	T1675	prep	to,map
R958	T1677	T1676	pobj	chromosome,to
R959	T1678	T1677	nummod	1,chromosome
R960	T1679	T1675	prep	within,map
R961	T1680	T1681	nummod	one,clone
R962	T1681	T1679	pobj	clone,within
R963	T1682	T1681	compound	BAC,clone
R964	T1683	T1684	punct	(,294I17
R965	T1684	T1681	parataxis	294I17,clone
R966	T1685	T1684	compound	RP23,294I17
R967	T1686	T1684	punct	-,294I17
R968	T1687	T1684	punct	),294I17
R969	T1688	T1681	punct	", ",clone
R970	T1689	T1681	amod	proximal,clone
R971	T1690	T1689	prep	to,proximal
R972	T1691	T1692	compound	marker,D1Mit171
R973	T1692	T1690	pobj	D1Mit171,to
R974	T1693	T1694	punct	(,cR
R975	T1694	T1692	parataxis	cR,D1Mit171
R976	T1695	T1694	nummod	17.4,cR
R977	T1696	T1694	punct	),cR
R978	T1697	T1675	punct	.,map
R979	T1699	T1700	det	These,regions
R980	T1700	T1701	nsubj	regions,are
R981	T1702	T1701	acomp	syntenic,are
R982	T1703	T1702	prep	to,syntenic
R983	T1704	T1705	det	the,counterparts
R984	T1705	T1703	pobj	counterparts,to
R985	T1706	T1705	amod	human,counterparts
R986	T1707	T1701	punct	.,are
R987	T2019	T2020	compound	Sequence,homology
R988	T2021	T2020	cc	and,homology
R989	T2022	T2023	amod	molecular,characteristics
R990	T2023	T2020	conj	characteristics,homology
R991	T2025	T2026	det	The,genes
R992	T2026	T2029	nsubj	genes,showed
R993	T2027	T2026	compound	mouse,genes
R994	T2028	T2026	compound	Acdp,genes
R995	T2030	T2031	advmod	very,strong
R996	T2031	T2032	amod	strong,homologies
R997	T2032	T2029	dobj	homologies,showed
R998	T2033	T2032	prep	of,homologies
R999	T2034	T2035	preconj	both,nucleotide

PMC:340383 / 1718-23691 JSONTXT 5 Projects

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PMC:340383 / 1718-23691 JSON TXT 5 Projects