PMC:340383 JSON TXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T216	0-9	JJ	denotes	Molecular
T218	0-68	sentence	denotes	Molecular cloning and characterization of the mouse Acdp gene family
T217	10-17	NN	denotes	cloning
T219	18-21	CC	denotes	and
T220	22-38	NN	denotes	characterization
T221	39-41	IN	denotes	of
T222	42-45	DT	denotes	the
T224	46-51	NN	denotes	mouse
T225	52-56	NN	denotes	Acdp
T226	57-61	NN	denotes	gene
T223	62-68	NN	denotes	family
T227	68-69	sentence	denotes
T228	79-89	sentence	denotes	Background
T229	79-89	NN	denotes	Background
T230	89-208	sentence	denotes	We have recently cloned and characterized a novel gene family named ancient conserved domain protein (ACDP) in humans.
T231	90-92	PRP	denotes	We
T233	93-97	VBP	denotes	have
T234	98-106	RB	denotes	recently
T232	107-113	VBN	denotes	cloned
T235	114-117	CC	denotes	and
T236	118-131	VBN	denotes	characterized
T237	132-133	DT	denotes	a
T239	134-139	JJ	denotes	novel
T240	140-144	NN	denotes	gene
T238	145-151	NN	denotes	family
T241	152-157	VBN	denotes	named
T242	158-165	JJ	denotes	ancient
T244	166-175	VBN	denotes	conserved
T245	176-182	NN	denotes	domain
T243	183-190	NN	denotes	protein
T246	191-192	-LRB-	denotes	(
T247	192-196	NN	denotes	ACDP
T248	196-197	-RRB-	denotes	)
T249	198-200	IN	denotes	in
T250	201-207	NNS	denotes	humans
T251	207-208	.	denotes	.
T252	208-360	sentence	denotes	To facilitate the functional study of this novel gene family, we have cloned and characterized Acdp, the mouse homologue of the human ACDP gene family.
T253	209-211	TO	denotes	To
T254	212-222	VB	denotes	facilitate
T256	223-226	DT	denotes	the
T258	227-237	JJ	denotes	functional
T257	238-243	NN	denotes	study
T259	244-246	IN	denotes	of
T260	247-251	DT	denotes	this
T262	252-257	JJ	denotes	novel
T263	258-262	NN	denotes	gene
T261	263-269	NN	denotes	family
T264	269-271	,	denotes	,
T265	271-273	PRP	denotes	we
T266	274-278	VBP	denotes	have
T255	279-285	VBN	denotes	cloned
T267	286-289	CC	denotes	and
T268	290-303	VBN	denotes	characterized
T269	304-308	NN	denotes	Acdp
T270	308-310	,	denotes	,
T271	310-313	DT	denotes	the
T273	314-319	NN	denotes	mouse
T272	320-329	NN	denotes	homologue
T274	330-332	IN	denotes	of
T275	333-336	DT	denotes	the
T277	337-342	JJ	denotes	human
T278	343-347	NN	denotes	ACDP
T279	348-352	NN	denotes	gene
T276	353-359	NN	denotes	family
T280	359-360	.	denotes	.
T281	360-369	sentence	denotes	Results
T282	362-369	NNS	denotes	Results
T283	369-570	sentence	denotes	The four Acdp genes (Acdp1, Acdp2, Acdp3 and Acdp4) contain 3,631 bp, 3,244 bp, 2,684 bp and 2,743 bp of cDNA sequences, and encode deduced proteins of 951, 874, 713 and 771 amino acids, respectively.
T284	370-373	DT	denotes	The
T286	374-378	CD	denotes	four
T287	379-383	NN	denotes	Acdp
T285	384-389	NNS	denotes	genes
T289	390-391	-LRB-	denotes	(
T290	391-396	NN	denotes	Acdp1
T291	396-398	,	denotes	,
T292	398-403	NN	denotes	Acdp2
T293	403-405	,	denotes	,
T294	405-410	NN	denotes	Acdp3
T295	411-414	CC	denotes	and
T296	415-420	NN	denotes	Acdp4
T297	420-421	-RRB-	denotes	)
T288	422-429	VBP	denotes	contain
T298	430-435	CD	denotes	3,631
T299	436-438	NNS	denotes	bp
T300	438-440	,	denotes	,
T301	440-445	CD	denotes	3,244
T302	446-448	NNS	denotes	bp
T303	448-450	,	denotes	,
T304	450-455	CD	denotes	2,684
T305	456-458	NNS	denotes	bp
T306	459-462	CC	denotes	and
T307	463-468	CD	denotes	2,743
T308	469-471	NNS	denotes	bp
T309	472-474	IN	denotes	of
T310	475-479	NN	denotes	cDNA
T311	480-489	NNS	denotes	sequences
T312	489-491	,	denotes	,
T313	491-494	CC	denotes	and
T314	495-501	VBP	denotes	encode
T315	502-509	VBN	denotes	deduced
T316	510-518	NN	denotes	proteins
T317	519-521	IN	denotes	of
T318	522-525	CD	denotes	951
T320	525-527	,	denotes	,
T321	527-530	CD	denotes	874
T322	530-532	,	denotes	,
T323	532-535	CD	denotes	713
T324	536-539	CC	denotes	and
T325	540-543	CD	denotes	771
T326	544-549	NN	denotes	amino
T319	550-555	NNS	denotes	acids
T327	555-557	,	denotes	,
T328	557-569	RB	denotes	respectively
T329	569-570	.	denotes	.
T330	570-701	sentence	denotes	The mouse Acdp genes showed very strong homologies (>90%) in both nucleotide and amino acid sequences to their human counterparts.
T331	571-574	DT	denotes	The
T333	575-580	NN	denotes	mouse
T334	581-585	NN	denotes	Acdp
T332	586-591	NNS	denotes	genes
T335	592-598	VBD	denotes	showed
T336	599-603	RB	denotes	very
T337	604-610	JJ	denotes	strong
T338	611-621	NNS	denotes	homologies
T339	622-623	-LRB-	denotes	(
T341	623-624	SYM	denotes	>
T342	624-626	CD	denotes	90
T340	626-627	NN	denotes	%
T343	627-628	-RRB-	denotes	)
T344	629-631	IN	denotes	in
T345	632-636	CC	denotes	both
T346	637-647	NN	denotes	nucleotide
T348	648-651	CC	denotes	and
T349	652-657	NN	denotes	amino
T350	658-662	NN	denotes	acid
T347	663-672	NNS	denotes	sequences
T351	673-675	IN	denotes	to
T352	676-681	PRP$	denotes	their
T354	682-687	JJ	denotes	human
T353	688-700	NNS	denotes	counterparts
T355	700-701	.	denotes	.
T356	701-855	sentence	denotes	In addition, both nucleotide and amino acid sequences within the Ancient Conserved Domain (ACD) are highly conserved in many different taxonomic species.
T357	702-704	IN	denotes	In
T359	705-713	NN	denotes	addition
T360	713-715	,	denotes	,
T361	715-719	CC	denotes	both
T362	720-730	NN	denotes	nucleotide
T364	731-734	CC	denotes	and
T365	735-740	NN	denotes	amino
T366	741-745	NN	denotes	acid
T363	746-755	NNS	denotes	sequences
T367	756-762	IN	denotes	within
T368	763-766	DT	denotes	the
T370	767-774	JJ	denotes	Ancient
T371	775-784	VBN	denotes	Conserved
T369	785-791	NN	denotes	Domain
T372	792-793	-LRB-	denotes	(
T373	793-796	NN	denotes	ACD
T374	796-797	-RRB-	denotes	)
T375	798-801	VBP	denotes	are
T376	802-808	RB	denotes	highly
T358	809-818	VBN	denotes	conserved
T377	819-821	IN	denotes	in
T378	822-826	JJ	denotes	many
T380	827-836	JJ	denotes	different
T381	837-846	JJ	denotes	taxonomic
T379	847-854	NNS	denotes	species
T382	854-855	.	denotes	.
T383	855-1032	sentence	denotes	Particularly, Acdp proteins showed very strong AA homologies to the bacteria CorC protein (35% AA identity with 55% homology), which is involved in magnesium and cobalt efflux.
T384	856-868	RB	denotes	Particularly
T386	868-870	,	denotes	,
T387	870-874	NN	denotes	Acdp
T388	875-883	NN	denotes	proteins
T385	884-890	VBD	denotes	showed
T389	891-895	RB	denotes	very
T390	896-902	JJ	denotes	strong
T392	903-905	NN	denotes	AA
T391	906-916	NNS	denotes	homologies
T393	917-919	IN	denotes	to
T394	920-923	DT	denotes	the
T396	924-932	NNS	denotes	bacteria
T397	933-937	NN	denotes	CorC
T395	938-945	NN	denotes	protein
T398	946-947	-LRB-	denotes	(
T400	947-949	CD	denotes	35
T401	949-950	NN	denotes	%
T402	951-953	NN	denotes	AA
T399	954-962	NN	denotes	identity
T403	963-967	IN	denotes	with
T404	968-970	CD	denotes	55
T405	970-971	NN	denotes	%
T406	972-980	NN	denotes	homology
T407	980-981	-RRB-	denotes	)
T408	981-983	,	denotes	,
T409	983-988	WDT	denotes	which
T411	989-991	VBZ	denotes	is
T410	992-1000	VBN	denotes	involved
T412	1001-1003	IN	denotes	in
T413	1004-1013	NN	denotes	magnesium
T415	1014-1017	CC	denotes	and
T416	1018-1024	NN	denotes	cobalt
T414	1025-1031	NN	denotes	efflux
T417	1031-1032	.	denotes	.
T418	1032-1204	sentence	denotes	The Acdp genes are widely expressed in all tissues tested except for Acdp1, which is only highly expressed in the brain with low levels of expression in kidney and testis.
T419	1033-1036	DT	denotes	The
T421	1037-1041	NN	denotes	Acdp
T420	1042-1047	NNS	denotes	genes
T423	1048-1051	VBP	denotes	are
T424	1052-1058	RB	denotes	widely
T422	1059-1068	VBN	denotes	expressed
T425	1069-1071	IN	denotes	in
T426	1072-1075	DT	denotes	all
T427	1076-1083	NNS	denotes	tissues
T428	1084-1090	VBN	denotes	tested
T429	1091-1097	IN	denotes	except
T430	1098-1101	IN	denotes	for
T431	1102-1107	NN	denotes	Acdp1
T432	1107-1109	,	denotes	,
T433	1109-1114	WDT	denotes	which
T435	1115-1117	VBZ	denotes	is
T436	1118-1122	RB	denotes	only
T437	1123-1129	RB	denotes	highly
T434	1130-1139	VBN	denotes	expressed
T438	1140-1142	IN	denotes	in
T439	1143-1146	DT	denotes	the
T440	1147-1152	NN	denotes	brain
T441	1153-1157	IN	denotes	with
T443	1158-1161	JJ	denotes	low
T442	1162-1168	NNS	denotes	levels
T444	1169-1171	IN	denotes	of
T445	1172-1182	NN	denotes	expression
T446	1183-1185	IN	denotes	in
T447	1186-1192	NN	denotes	kidney
T448	1193-1196	CC	denotes	and
T449	1197-1203	NN	denotes	testis
T450	1203-1204	.	denotes	.
T451	1204-1311	sentence	denotes	Immunostaining of Acdp1 in hippocampus neurons revealed a predominant localization on the plasma membrane.
T452	1205-1219	NN	denotes	Immunostaining
T454	1220-1222	IN	denotes	of
T455	1223-1228	NN	denotes	Acdp1
T456	1229-1231	IN	denotes	in
T457	1232-1243	NN	denotes	hippocampus
T458	1244-1251	NNS	denotes	neurons
T453	1252-1260	VBD	denotes	revealed
T459	1261-1262	DT	denotes	a
T461	1263-1274	JJ	denotes	predominant
T460	1275-1287	NN	denotes	localization
T462	1288-1290	IN	denotes	on
T463	1291-1294	DT	denotes	the
T465	1295-1301	NN	denotes	plasma
T464	1302-1310	NN	denotes	membrane
T466	1310-1311	.	denotes	.
T467	1311-1323	sentence	denotes	Conclusion
T468	1313-1323	NN	denotes	Conclusion
T469	1323-1501	sentence	denotes	The Acdp genes are evolutionarily conserved in diverse species and ubiquitously expressed throughout development and adult tissues suggesting that Acdp may be an essential gene.
T470	1324-1327	DT	denotes	The
T472	1328-1332	NN	denotes	Acdp
T471	1333-1338	NNS	denotes	genes
T474	1339-1342	VBP	denotes	are
T475	1343-1357	RB	denotes	evolutionarily
T473	1358-1367	VBN	denotes	conserved
T476	1368-1370	IN	denotes	in
T477	1371-1378	JJ	denotes	diverse
T478	1379-1386	NNS	denotes	species
T479	1387-1390	CC	denotes	and
T480	1391-1403	RB	denotes	ubiquitously
T481	1404-1413	VBN	denotes	expressed
T482	1414-1424	IN	denotes	throughout
T483	1425-1436	NN	denotes	development
T484	1437-1440	CC	denotes	and
T485	1441-1446	JJ	denotes	adult
T486	1447-1454	NNS	denotes	tissues
T487	1455-1465	VBG	denotes	suggesting
T488	1466-1470	IN	denotes	that
T490	1471-1475	NN	denotes	Acdp
T491	1476-1479	MD	denotes	may
T489	1480-1482	VB	denotes	be
T492	1483-1485	DT	denotes	an
T494	1486-1495	JJ	denotes	essential
T493	1496-1500	NN	denotes	gene
T495	1500-1501	.	denotes	.
T496	1501-1606	sentence	denotes	Acdp showed strong homology to bacteria CorC protein and predominantly localized on the plasma membrane.
T497	1502-1506	NN	denotes	Acdp
T498	1507-1513	VBD	denotes	showed
T499	1514-1520	JJ	denotes	strong
T500	1521-1529	NN	denotes	homology
T501	1530-1532	IN	denotes	to
T502	1533-1541	NNS	denotes	bacteria
T504	1542-1546	NN	denotes	CorC
T503	1547-1554	NN	denotes	protein
T505	1555-1558	CC	denotes	and
T506	1559-1572	RB	denotes	predominantly
T507	1573-1582	VBD	denotes	localized
T508	1583-1585	IN	denotes	on
T509	1586-1589	DT	denotes	the
T511	1590-1596	NN	denotes	plasma
T510	1597-1605	NN	denotes	membrane
T512	1605-1606	.	denotes	.
T513	1606-1716	sentence	denotes	These results suggest that Acdp is probably a family of proteins involved in ion transport in mammalian cells
T514	1607-1612	DT	denotes	These
T515	1613-1620	NNS	denotes	results
T516	1621-1628	VBP	denotes	suggest
T517	1629-1633	IN	denotes	that
T519	1634-1638	NN	denotes	Acdp
T518	1639-1641	VBZ	denotes	is
T520	1642-1650	RB	denotes	probably
T521	1651-1652	DT	denotes	a
T522	1653-1659	NN	denotes	family
T523	1660-1662	IN	denotes	of
T524	1663-1671	NN	denotes	proteins
T525	1672-1680	VBN	denotes	involved
T526	1681-1683	IN	denotes	in
T527	1684-1687	NN	denotes	ion
T528	1688-1697	NN	denotes	transport
T529	1698-1700	IN	denotes	in
T530	1701-1710	JJ	denotes	mammalian
T531	1711-1716	NNS	denotes	cells
T593	1729-1731	PRP	denotes	We
T595	1732-1736	VBP	denotes	have
T596	1737-1745	RB	denotes	recently
T594	1746-1752	VBN	denotes	cloned
T597	1753-1756	CC	denotes	and
T598	1757-1770	VBN	denotes	characterized
T599	1771-1772	DT	denotes	a
T601	1773-1778	JJ	denotes	novel
T602	1779-1783	NN	denotes	gene
T600	1784-1790	NN	denotes	family
T603	1791-1796	VBN	denotes	named
T604	1797-1804	JJ	denotes	ancient
T606	1805-1814	VBN	denotes	conserved
T607	1815-1821	NN	denotes	domain
T605	1822-1829	NN	denotes	protein
T608	1830-1831	-LRB-	denotes	(
T609	1831-1835	NN	denotes	ACDP
T610	1835-1836	-RRB-	denotes	)
T611	1837-1842	WDT	denotes	which
T612	1843-1850	VBZ	denotes	encodes
T613	1851-1855	CD	denotes	four
T615	1856-1863	NN	denotes	protein
T614	1864-1871	NNS	denotes	members
T616	1872-1874	IN	denotes	in
T617	1875-1881	NNS	denotes	humans
T618	1882-1883	-LRB-	denotes	[
T619	1883-1884	CD	denotes	1
T620	1884-1885	-RRB-	denotes	]
T621	1885-1886	.	denotes	.
T622	1886-2038	sentence	denotes	We found that this gene family is evolutionarily conserved in diverse species ranging from bacteria, yeast, C. elegans, and D. melanogaster to mammals.
T623	1887-1889	PRP	denotes	We
T624	1890-1895	VBD	denotes	found
T625	1896-1900	IN	denotes	that
T627	1901-1905	DT	denotes	this
T629	1906-1910	NN	denotes	gene
T628	1911-1917	NN	denotes	family
T626	1918-1920	VBZ	denotes	is
T630	1921-1935	RB	denotes	evolutionarily
T631	1936-1945	JJ	denotes	conserved
T632	1946-1948	IN	denotes	in
T633	1949-1956	JJ	denotes	diverse
T634	1957-1964	NNS	denotes	species
T635	1965-1972	VBG	denotes	ranging
T636	1973-1977	IN	denotes	from
T637	1978-1986	NNS	denotes	bacteria
T638	1986-1988	,	denotes	,
T639	1988-1993	NN	denotes	yeast
T640	1993-1995	,	denotes	,
T641	1995-1997	NNP	denotes	C.
T642	1998-2005	NNP	denotes	elegans
T643	2005-2007	,	denotes	,
T644	2007-2010	CC	denotes	and
T645	2011-2013	NNP	denotes	D.
T646	2014-2026	NNP	denotes	melanogaster
T647	2027-2029	IN	denotes	to
T648	2030-2037	NNS	denotes	mammals
T649	2037-2038	.	denotes	.
T650	2038-2177	sentence	denotes	The sequence conservation and the presence of multiple members within a species may imply functional importance associated with the genes.
T651	2039-2042	DT	denotes	The
T653	2043-2051	NN	denotes	sequence
T652	2052-2064	NN	denotes	conservation
T655	2065-2068	CC	denotes	and
T656	2069-2072	DT	denotes	the
T657	2073-2081	NN	denotes	presence
T658	2082-2084	IN	denotes	of
T659	2085-2093	JJ	denotes	multiple
T660	2094-2101	NNS	denotes	members
T661	2102-2108	IN	denotes	within
T662	2109-2110	DT	denotes	a
T663	2111-2118	NN	denotes	species
T664	2119-2122	MD	denotes	may
T654	2123-2128	VB	denotes	imply
T665	2129-2139	JJ	denotes	functional
T666	2140-2150	NN	denotes	importance
T667	2151-2161	VBN	denotes	associated
T668	2162-2166	IN	denotes	with
T669	2167-2170	DT	denotes	the
T670	2171-2176	NNS	denotes	genes
T671	2176-2177	.	denotes	.
T672	2177-2325	sentence	denotes	To facilitate the functional analysis of the ACDP gene family, we cloned and characterized Acdp, the mouse homologue of the human ACDP gene family.
T673	2178-2180	TO	denotes	To
T674	2181-2191	VB	denotes	facilitate
T676	2192-2195	DT	denotes	the
T678	2196-2206	JJ	denotes	functional
T677	2207-2215	NN	denotes	analysis
T679	2216-2218	IN	denotes	of
T680	2219-2222	DT	denotes	the
T682	2223-2227	NN	denotes	ACDP
T683	2228-2232	NN	denotes	gene
T681	2233-2239	NN	denotes	family
T684	2239-2241	,	denotes	,
T685	2241-2243	PRP	denotes	we
T675	2244-2250	VBD	denotes	cloned
T686	2251-2254	CC	denotes	and
T687	2255-2268	VBD	denotes	characterized
T688	2269-2273	NN	denotes	Acdp
T689	2273-2275	,	denotes	,
T690	2275-2278	DT	denotes	the
T692	2279-2284	NN	denotes	mouse
T691	2285-2294	NN	denotes	homologue
T693	2295-2297	IN	denotes	of
T694	2298-2301	DT	denotes	the
T696	2302-2307	JJ	denotes	human
T697	2308-2312	NN	denotes	ACDP
T698	2313-2317	NN	denotes	gene
T695	2318-2324	NN	denotes	family
T699	2324-2325	.	denotes	.
T863	2336-2345	JJ	denotes	Molecular
T864	2346-2353	NN	denotes	cloning
T865	2354-2356	IN	denotes	of
T866	2357-2360	DT	denotes	the
T868	2361-2365	NN	denotes	Acdp
T869	2366-2370	NN	denotes	gene
T867	2371-2377	NN	denotes	family
T870	2377-2541	sentence	denotes	To clone the mouse Acdp genes, the human ACDP cDNA and predicted protein sequences were used to search the mouse EST database with the blastn and tblastn programs.
T871	2378-2380	TO	denotes	To
T872	2381-2386	VB	denotes	clone
T874	2387-2390	DT	denotes	the
T876	2391-2396	NN	denotes	mouse
T877	2397-2401	NN	denotes	Acdp
T875	2402-2407	NNS	denotes	genes
T878	2407-2409	,	denotes	,
T879	2409-2412	DT	denotes	the
T881	2413-2418	JJ	denotes	human
T882	2419-2423	NN	denotes	ACDP
T880	2424-2428	NN	denotes	cDNA
T883	2429-2432	CC	denotes	and
T884	2433-2442	JJ	denotes	predicted
T886	2443-2450	NN	denotes	protein
T885	2451-2460	NNS	denotes	sequences
T887	2461-2465	VBD	denotes	were
T873	2466-2470	VBN	denotes	used
T888	2471-2473	TO	denotes	to
T889	2474-2480	VB	denotes	search
T890	2481-2484	DT	denotes	the
T892	2485-2490	NN	denotes	mouse
T893	2491-2494	NN	denotes	EST
T891	2495-2503	NN	denotes	database
T894	2504-2508	IN	denotes	with
T895	2509-2512	DT	denotes	the
T897	2513-2519	NN	denotes	blastn
T898	2520-2523	CC	denotes	and
T899	2524-2531	NN	denotes	tblastn
T896	2532-2540	NNS	denotes	programs
T900	2540-2541	.	denotes	.
T901	2541-2615	sentence	denotes	Mouse EST markers corresponding to each Acdp member were then identified.
T902	2542-2547	NN	denotes	Mouse
T904	2548-2551	NN	denotes	EST
T903	2552-2559	NNS	denotes	markers
T906	2560-2573	VBG	denotes	corresponding
T907	2574-2576	IN	denotes	to
T908	2577-2581	DT	denotes	each
T910	2582-2586	NN	denotes	Acdp
T909	2587-2593	NN	denotes	member
T911	2594-2598	VBD	denotes	were
T912	2599-2603	RB	denotes	then
T905	2604-2614	VBN	denotes	identified
T913	2614-2615	.	denotes	.
T914	2615-2729	sentence	denotes	For example, EST H3086H12-5 corresponds to Acdp1, W98010 for Acdp2, 603299135F1 for Acdp3 and BG083791 for Acdp4.
T915	2616-2619	IN	denotes	For
T917	2620-2627	NN	denotes	example
T918	2627-2629	,	denotes	,
T919	2629-2632	NN	denotes	EST
T920	2633-2641	NN	denotes	H3086H12
T921	2641-2642	HYPH	denotes	-
T922	2642-2643	CD	denotes	5
T916	2644-2655	VBZ	denotes	corresponds
T923	2656-2658	IN	denotes	to
T924	2659-2664	NN	denotes	Acdp1
T925	2664-2666	,	denotes	,
T926	2666-2672	NN	denotes	W98010
T927	2673-2676	IN	denotes	for
T928	2677-2682	NN	denotes	Acdp2
T929	2682-2684	,	denotes	,
T930	2684-2695	NN	denotes	603299135F1
T931	2696-2699	IN	denotes	for
T932	2700-2705	NN	denotes	Acdp3
T933	2706-2709	CC	denotes	and
T934	2710-2718	NN	denotes	BG083791
T935	2719-2722	IN	denotes	for
T936	2723-2728	NN	denotes	Acdp4
T937	2728-2729	.	denotes	.
T938	2729-2795	sentence	denotes	A modified oligo-dT with a M13 tail was used for the RT reaction.
T939	2730-2731	DT	denotes	A
T941	2732-2740	VBN	denotes	modified
T942	2741-2746	NN	denotes	oligo
T943	2746-2747	HYPH	denotes	-
T940	2747-2749	NN	denotes	dT
T945	2750-2754	IN	denotes	with
T946	2755-2756	DT	denotes	a
T948	2757-2760	NN	denotes	M13
T947	2761-2765	NN	denotes	tail
T949	2766-2769	VBD	denotes	was
T944	2770-2774	VBN	denotes	used
T950	2775-2778	IN	denotes	for
T951	2779-2782	DT	denotes	the
T953	2783-2785	NN	denotes	RT
T952	2786-2794	NN	denotes	reaction
T954	2794-2795	.	denotes	.
T955	2795-2963	sentence	denotes	A forward primer from each EST marker and the M13 primer (olig-dT tail) were used to amplify the 3' UTR sequence for each corresponding Acdp gene from the RT products.
T956	2796-2797	DT	denotes	A
T958	2798-2805	JJ	denotes	forward
T957	2806-2812	NN	denotes	primer
T960	2813-2817	IN	denotes	from
T961	2818-2822	DT	denotes	each
T963	2823-2826	NN	denotes	EST
T962	2827-2833	NN	denotes	marker
T964	2834-2837	CC	denotes	and
T965	2838-2841	DT	denotes	the
T967	2842-2845	NN	denotes	M13
T966	2846-2852	NN	denotes	primer
T968	2853-2854	-LRB-	denotes	(
T969	2854-2858	NN	denotes	olig
T971	2858-2859	HYPH	denotes	-
T970	2859-2861	NN	denotes	dT
T972	2862-2866	NN	denotes	tail
T973	2866-2867	-RRB-	denotes	)
T974	2868-2872	VBD	denotes	were
T959	2873-2877	VBN	denotes	used
T975	2878-2880	TO	denotes	to
T976	2881-2888	VB	denotes	amplify
T977	2889-2892	DT	denotes	the
T979	2893-2894	CD	denotes	3
T981	2894-2895	SYM	denotes	'
T980	2896-2899	NN	denotes	UTR
T978	2900-2908	NN	denotes	sequence
T982	2909-2912	IN	denotes	for
T983	2913-2917	DT	denotes	each
T985	2918-2931	VBG	denotes	corresponding
T986	2932-2936	NN	denotes	Acdp
T984	2937-2941	NN	denotes	gene
T987	2942-2946	IN	denotes	from
T988	2947-2950	DT	denotes	the
T990	2951-2953	NN	denotes	RT
T989	2954-2962	NNS	denotes	products
T991	2962-2963	.	denotes	.
T992	2963-3096	sentence	denotes	To obtain 5'-end coding sequences for the Acdp genes, we conducted a series nested PCR with combinations of mouse and human primers.
T993	2964-2966	TO	denotes	To
T994	2967-2973	VB	denotes	obtain
T996	2974-2975	CD	denotes	5
T998	2975-2976	SYM	denotes	'
T999	2976-2977	HYPH	denotes	-
T997	2977-2980	NN	denotes	end
T1001	2981-2987	VBG	denotes	coding
T1000	2988-2997	NNS	denotes	sequences
T1002	2998-3001	IN	denotes	for
T1003	3002-3005	DT	denotes	the
T1005	3006-3010	NN	denotes	Acdp
T1004	3011-3016	NNS	denotes	genes
T1006	3016-3018	,	denotes	,
T1007	3018-3020	PRP	denotes	we
T995	3021-3030	VBD	denotes	conducted
T1008	3031-3032	DT	denotes	a
T1010	3033-3039	NN	denotes	series
T1011	3040-3046	VBN	denotes	nested
T1009	3047-3050	NN	denotes	PCR
T1012	3051-3055	IN	denotes	with
T1013	3056-3068	NNS	denotes	combinations
T1014	3069-3071	IN	denotes	of
T1015	3072-3077	NN	denotes	mouse
T1017	3078-3081	CC	denotes	and
T1018	3082-3087	JJ	denotes	human
T1016	3088-3095	NNS	denotes	primers
T1019	3095-3096	.	denotes	.
T1020	3096-3205	sentence	denotes	The 5' UTR sequences were identified by directly sequencing BAC DNA containing the corresponding Acdp genes.
T1021	3097-3100	DT	denotes	The
T1023	3101-3102	CD	denotes	5
T1024	3102-3103	SYM	denotes	'
T1025	3104-3107	NN	denotes	UTR
T1022	3108-3117	NNS	denotes	sequences
T1027	3118-3122	VBD	denotes	were
T1026	3123-3133	VBN	denotes	identified
T1028	3134-3136	IN	denotes	by
T1029	3137-3145	RB	denotes	directly
T1030	3146-3156	VBG	denotes	sequencing
T1031	3157-3160	NN	denotes	BAC
T1032	3161-3164	NN	denotes	DNA
T1033	3165-3175	VBG	denotes	containing
T1034	3176-3179	DT	denotes	the
T1036	3180-3193	VBG	denotes	corresponding
T1037	3194-3198	NN	denotes	Acdp
T1035	3199-3204	NNS	denotes	genes
T1038	3204-3205	.	denotes	.
T1039	3205-3299	sentence	denotes	The BAC clones were identified by screening a CITB mouse BAC DNA library (Research Genetics).
T1040	3206-3209	DT	denotes	The
T1042	3210-3213	NN	denotes	BAC
T1041	3214-3220	NNS	denotes	clones
T1044	3221-3225	VBD	denotes	were
T1043	3226-3236	VBN	denotes	identified
T1045	3237-3239	IN	denotes	by
T1046	3240-3249	VBG	denotes	screening
T1047	3250-3251	DT	denotes	a
T1049	3252-3256	NN	denotes	CITB
T1051	3257-3262	NN	denotes	mouse
T1052	3263-3266	NN	denotes	BAC
T1050	3267-3270	NN	denotes	DNA
T1048	3271-3278	NN	denotes	library
T1053	3279-3280	-LRB-	denotes	(
T1055	3280-3288	NNP	denotes	Research
T1054	3289-3297	NNP	denotes	Genetics
T1056	3297-3298	-RRB-	denotes	)
T1057	3298-3299	.	denotes	.
T1058	3299-3374	sentence	denotes	The 5' UTR sequences obtained from above were further confirmed by RT-PCR.
T1059	3300-3303	DT	denotes	The
T1061	3304-3305	CD	denotes	5
T1062	3305-3306	SYM	denotes	'
T1063	3307-3310	NN	denotes	UTR
T1060	3311-3320	NNS	denotes	sequences
T1065	3321-3329	VBN	denotes	obtained
T1066	3330-3334	IN	denotes	from
T1067	3335-3340	RB	denotes	above
T1068	3341-3345	VBD	denotes	were
T1069	3346-3353	RB	denotes	further
T1064	3354-3363	VBN	denotes	confirmed
T1070	3364-3366	IN	denotes	by
T1071	3367-3369	NN	denotes	RT
T1073	3369-3370	HYPH	denotes	-
T1072	3370-3373	NN	denotes	PCR
T1074	3373-3374	.	denotes	.
T1075	3374-3489	sentence	denotes	The Acdp1 gene contains 3,631 bp of nucleotide sequence and encodes a predicted protein with 951 amino acids (AA).
T1076	3375-3378	DT	denotes	The
T1078	3379-3384	NN	denotes	Acdp1
T1077	3385-3389	NN	denotes	gene
T1079	3390-3398	VBZ	denotes	contains
T1080	3399-3404	CD	denotes	3,631
T1081	3405-3407	NNS	denotes	bp
T1082	3408-3410	IN	denotes	of
T1083	3411-3421	NN	denotes	nucleotide
T1084	3422-3430	NN	denotes	sequence
T1085	3431-3434	CC	denotes	and
T1086	3435-3442	VBZ	denotes	encodes
T1087	3443-3444	DT	denotes	a
T1089	3445-3454	VBN	denotes	predicted
T1088	3455-3462	NN	denotes	protein
T1090	3463-3467	IN	denotes	with
T1091	3468-3471	CD	denotes	951
T1093	3472-3477	NN	denotes	amino
T1092	3478-3483	NNS	denotes	acids
T1094	3484-3485	-LRB-	denotes	(
T1095	3485-3487	NN	denotes	AA
T1096	3487-3488	-RRB-	denotes	)
T1097	3488-3489	.	denotes	.
T1098	3489-3691	sentence	denotes	The other three Acdp genes (Acdp2, 3 and 4) contain 3,244 bp, 2,684 bp and 2,743 bp of cDNA sequences, and encode deduced proteins of 874 amino acids, 713 amino acids and 771 amino acids, respectively.
T1099	3490-3493	DT	denotes	The
T1101	3494-3499	JJ	denotes	other
T1102	3500-3505	CD	denotes	three
T1103	3506-3510	NN	denotes	Acdp
T1100	3511-3516	NNS	denotes	genes
T1105	3517-3518	-LRB-	denotes	(
T1106	3518-3523	NN	denotes	Acdp2
T1107	3523-3525	,	denotes	,
T1108	3525-3526	CD	denotes	3
T1109	3527-3530	CC	denotes	and
T1110	3531-3532	CD	denotes	4
T1111	3532-3533	-RRB-	denotes	)
T1104	3534-3541	VBP	denotes	contain
T1112	3542-3547	CD	denotes	3,244
T1113	3548-3550	NNS	denotes	bp
T1114	3550-3552	,	denotes	,
T1115	3552-3557	CD	denotes	2,684
T1116	3558-3560	NNS	denotes	bp
T1117	3561-3564	CC	denotes	and
T1118	3565-3570	CD	denotes	2,743
T1119	3571-3573	NNS	denotes	bp
T1120	3574-3576	IN	denotes	of
T1121	3577-3581	NN	denotes	cDNA
T1122	3582-3591	NNS	denotes	sequences
T1123	3591-3593	,	denotes	,
T1124	3593-3596	CC	denotes	and
T1125	3597-3603	VBP	denotes	encode
T1126	3604-3611	VBN	denotes	deduced
T1127	3612-3620	NN	denotes	proteins
T1128	3621-3623	IN	denotes	of
T1129	3624-3627	CD	denotes	874
T1131	3628-3633	NN	denotes	amino
T1130	3634-3639	NNS	denotes	acids
T1132	3639-3641	,	denotes	,
T1133	3641-3644	CD	denotes	713
T1135	3645-3650	NN	denotes	amino
T1134	3651-3656	NNS	denotes	acids
T1136	3657-3660	CC	denotes	and
T1137	3661-3664	CD	denotes	771
T1139	3665-3670	NN	denotes	amino
T1138	3671-3676	NNS	denotes	acids
T1140	3676-3678	,	denotes	,
T1141	3678-3690	RB	denotes	respectively
T1142	3690-3691	.	denotes	.
T1296	3693-3699	NN	denotes	Tissue
T1297	3700-3712	NN	denotes	distribution
T1298	3712-3816	sentence	denotes	Northern blot analyses of the Acdp gene family were carried out using membranes purchased from Origene.
T1299	3713-3721	NNP	denotes	Northern
T1300	3722-3726	NN	denotes	blot
T1301	3727-3735	NNS	denotes	analyses
T1303	3736-3738	IN	denotes	of
T1304	3739-3742	DT	denotes	the
T1306	3743-3747	NN	denotes	Acdp
T1307	3748-3752	NN	denotes	gene
T1305	3753-3759	NN	denotes	family
T1308	3760-3764	VBD	denotes	were
T1302	3765-3772	VBN	denotes	carried
T1309	3773-3776	RP	denotes	out
T1310	3777-3782	VBG	denotes	using
T1311	3783-3792	NNS	denotes	membranes
T1312	3793-3802	VBN	denotes	purchased
T1313	3803-3807	IN	denotes	from
T1314	3808-3815	NNP	denotes	Origene
T1315	3815-3816	.	denotes	.
T1316	3816-3881	sentence	denotes	A total of 12 mouse tissues were included in the study (Fig. 1).
T1317	3817-3818	DT	denotes	A
T1318	3819-3824	NN	denotes	total
T1320	3825-3827	IN	denotes	of
T1321	3828-3830	CD	denotes	12
T1323	3831-3836	NN	denotes	mouse
T1322	3837-3844	NNS	denotes	tissues
T1324	3845-3849	VBD	denotes	were
T1319	3850-3858	VBN	denotes	included
T1325	3859-3861	IN	denotes	in
T1326	3862-3865	DT	denotes	the
T1327	3866-3871	NN	denotes	study
T1328	3872-3873	-LRB-	denotes	(
T1329	3873-3877	NN	denotes	Fig.
T1330	3878-3879	CD	denotes	1
T1331	3879-3880	-RRB-	denotes	)
T1332	3880-3881	.	denotes	.
T1333	3881-4068	sentence	denotes	Due to sequence homologies between each Acdp member within the conserved domain, probes for Northern bolts were PCR fragments from the last exon and the 3' untranslated region sequences.
T1334	3882-3885	IN	denotes	Due
T1336	3886-3888	IN	denotes	to
T1337	3889-3897	NN	denotes	sequence
T1338	3898-3908	NNS	denotes	homologies
T1339	3909-3916	IN	denotes	between
T1340	3917-3921	DT	denotes	each
T1342	3922-3926	NN	denotes	Acdp
T1341	3927-3933	NN	denotes	member
T1343	3934-3940	IN	denotes	within
T1344	3941-3944	DT	denotes	the
T1346	3945-3954	VBN	denotes	conserved
T1345	3955-3961	NN	denotes	domain
T1347	3961-3963	,	denotes	,
T1348	3963-3969	NNS	denotes	probes
T1349	3970-3973	IN	denotes	for
T1350	3974-3982	NNP	denotes	Northern
T1351	3983-3988	NNS	denotes	bolts
T1335	3989-3993	VBD	denotes	were
T1352	3994-3997	NN	denotes	PCR
T1353	3998-4007	NNS	denotes	fragments
T1354	4008-4012	IN	denotes	from
T1355	4013-4016	DT	denotes	the
T1357	4017-4021	JJ	denotes	last
T1356	4022-4026	NN	denotes	exon
T1358	4027-4030	CC	denotes	and
T1359	4031-4034	DT	denotes	the
T1361	4035-4036	CD	denotes	3
T1363	4036-4037	SYM	denotes	'
T1364	4038-4050	JJ	denotes	untranslated
T1362	4051-4057	NN	denotes	region
T1360	4058-4067	NNS	denotes	sequences
T1365	4067-4068	.	denotes	.
T1366	4068-4161	sentence	denotes	The mouse Acdp messages showed almost the same tissue distributions as the human ACDP genes.
T1367	4069-4072	DT	denotes	The
T1369	4073-4078	NN	denotes	mouse
T1370	4079-4083	NN	denotes	Acdp
T1368	4084-4092	NNS	denotes	messages
T1371	4093-4099	VBD	denotes	showed
T1372	4100-4106	RB	denotes	almost
T1374	4107-4110	DT	denotes	the
T1375	4111-4115	JJ	denotes	same
T1376	4116-4122	NN	denotes	tissue
T1373	4123-4136	NNS	denotes	distributions
T1377	4137-4139	IN	denotes	as
T1378	4140-4143	DT	denotes	the
T1380	4144-4149	JJ	denotes	human
T1381	4150-4154	NN	denotes	ACDP
T1379	4155-4160	NNS	denotes	genes
T1382	4160-4161	.	denotes	.
T1383	4161-4271	sentence	denotes	Acdp1 message is highly expressed in the brain, while kidney and testis also showed low levels of expression.
T1384	4162-4167	NN	denotes	Acdp1
T1385	4168-4175	NN	denotes	message
T1387	4176-4178	VBZ	denotes	is
T1388	4179-4185	RB	denotes	highly
T1386	4186-4195	VBN	denotes	expressed
T1389	4196-4198	IN	denotes	in
T1390	4199-4202	DT	denotes	the
T1391	4203-4208	NN	denotes	brain
T1392	4208-4210	,	denotes	,
T1393	4210-4215	IN	denotes	while
T1395	4216-4222	NN	denotes	kidney
T1396	4223-4226	CC	denotes	and
T1397	4227-4233	NN	denotes	testis
T1398	4234-4238	RB	denotes	also
T1394	4239-4245	VBD	denotes	showed
T1399	4246-4249	JJ	denotes	low
T1400	4250-4256	NNS	denotes	levels
T1401	4257-4259	IN	denotes	of
T1402	4260-4270	NN	denotes	expression
T1403	4270-4271	.	denotes	.
T1404	4271-4335	sentence	denotes	Acdp2 showed higher expressions in the brain, kidney and liver.
T1405	4272-4277	NN	denotes	Acdp2
T1406	4278-4284	VBD	denotes	showed
T1407	4285-4291	JJR	denotes	higher
T1408	4292-4303	NNS	denotes	expressions
T1409	4304-4306	IN	denotes	in
T1410	4307-4310	DT	denotes	the
T1411	4311-4316	NN	denotes	brain
T1412	4316-4318	,	denotes	,
T1413	4318-4324	NN	denotes	kidney
T1414	4325-4328	CC	denotes	and
T1415	4329-4334	NN	denotes	liver
T1416	4334-4335	.	denotes	.
T1417	4335-4482	sentence	denotes	However, the Acdp2 transcript was not present in the skeleton muscle and skin, and it showed very low levels of expression in the rest of tissues.
T1418	4336-4343	RB	denotes	However
T1420	4343-4345	,	denotes	,
T1421	4345-4348	DT	denotes	the
T1423	4349-4354	NN	denotes	Acdp2
T1422	4355-4365	NN	denotes	transcript
T1419	4366-4369	VBD	denotes	was
T1424	4370-4373	RB	denotes	not
T1425	4374-4381	JJ	denotes	present
T1426	4382-4384	IN	denotes	in
T1427	4385-4388	DT	denotes	the
T1429	4389-4397	NN	denotes	skeleton
T1428	4398-4404	NN	denotes	muscle
T1430	4405-4408	CC	denotes	and
T1431	4409-4413	NN	denotes	skin
T1432	4413-4415	,	denotes	,
T1433	4415-4418	CC	denotes	and
T1434	4419-4421	PRP	denotes	it
T1435	4422-4428	VBD	denotes	showed
T1436	4429-4433	RB	denotes	very
T1437	4434-4437	JJ	denotes	low
T1438	4438-4444	NNS	denotes	levels
T1439	4445-4447	IN	denotes	of
T1440	4448-4458	NN	denotes	expression
T1441	4459-4461	IN	denotes	in
T1442	4462-4465	DT	denotes	the
T1443	4466-4470	NN	denotes	rest
T1444	4471-4473	IN	denotes	of
T1445	4474-4481	NNS	denotes	tissues
T1446	4481-4482	.	denotes	.
T1447	4482-4741	sentence	denotes	Acdp3 and Acdp4 showed different levels of expression in all tissues tested; the highest expressions for Acdp3 were observed in the brain, kidney, liver and heart, and the highest expressions for Acdp4 were observed in the kidney, small intestine and testis.
T1448	4483-4488	NN	denotes	Acdp3
T1450	4489-4492	CC	denotes	and
T1451	4493-4498	NN	denotes	Acdp4
T1449	4499-4505	VBD	denotes	showed
T1453	4506-4515	JJ	denotes	different
T1454	4516-4522	NNS	denotes	levels
T1455	4523-4525	IN	denotes	of
T1456	4526-4536	NN	denotes	expression
T1457	4537-4539	IN	denotes	in
T1458	4540-4543	DT	denotes	all
T1459	4544-4551	NNS	denotes	tissues
T1460	4552-4558	VBN	denotes	tested
T1461	4558-4559	:	denotes	;
T1462	4560-4563	DT	denotes	the
T1464	4564-4571	JJS	denotes	highest
T1463	4572-4583	NNS	denotes	expressions
T1465	4584-4587	IN	denotes	for
T1466	4588-4593	NN	denotes	Acdp3
T1467	4594-4598	VBD	denotes	were
T1452	4599-4607	VBN	denotes	observed
T1468	4608-4610	IN	denotes	in
T1469	4611-4614	DT	denotes	the
T1470	4615-4620	NN	denotes	brain
T1471	4620-4622	,	denotes	,
T1472	4622-4628	NN	denotes	kidney
T1473	4628-4630	,	denotes	,
T1474	4630-4635	NN	denotes	liver
T1475	4636-4639	CC	denotes	and
T1476	4640-4645	NN	denotes	heart
T1477	4645-4647	,	denotes	,
T1478	4647-4650	CC	denotes	and
T1479	4651-4654	DT	denotes	the
T1481	4655-4662	JJS	denotes	highest
T1480	4663-4674	NNS	denotes	expressions
T1483	4675-4678	IN	denotes	for
T1484	4679-4684	NN	denotes	Acdp4
T1485	4685-4689	VBD	denotes	were
T1482	4690-4698	VBN	denotes	observed
T1486	4699-4701	IN	denotes	in
T1487	4702-4705	DT	denotes	the
T1488	4706-4712	NN	denotes	kidney
T1489	4712-4714	,	denotes	,
T1490	4714-4719	JJ	denotes	small
T1491	4720-4729	NN	denotes	intestine
T1492	4730-4733	CC	denotes	and
T1493	4734-4740	NN	denotes	testis
T1494	4740-4741	.	denotes	.
T1495	4741-4952	sentence	denotes	The expression levels for Acdp3 and 4 in skeleton muscle were barely detectable; however, β-actin showed normal expression suggesting that the results were not a consequence of bad RNA quality (data not shown).
T1496	4742-4745	DT	denotes	The
T1498	4746-4756	NN	denotes	expression
T1497	4757-4763	NNS	denotes	levels
T1500	4764-4767	IN	denotes	for
T1501	4768-4773	NN	denotes	Acdp3
T1502	4774-4777	CC	denotes	and
T1503	4778-4779	CD	denotes	4
T1504	4780-4782	IN	denotes	in
T1505	4783-4791	NN	denotes	skeleton
T1506	4792-4798	NN	denotes	muscle
T1499	4799-4803	VBD	denotes	were
T1508	4804-4810	RB	denotes	barely
T1509	4811-4821	JJ	denotes	detectable
T1510	4821-4822	:	denotes	;
T1511	4823-4830	RB	denotes	however
T1512	4830-4832	,	denotes	,
T1513	4832-4833	NN	denotes	β
T1515	4833-4834	HYPH	denotes	-
T1514	4834-4839	NN	denotes	actin
T1507	4840-4846	VBD	denotes	showed
T1516	4847-4853	JJ	denotes	normal
T1517	4854-4864	NN	denotes	expression
T1518	4865-4875	VBG	denotes	suggesting
T1519	4876-4880	IN	denotes	that
T1521	4881-4884	DT	denotes	the
T1522	4885-4892	NNS	denotes	results
T1520	4893-4897	VBD	denotes	were
T1523	4898-4901	RB	denotes	not
T1524	4902-4903	DT	denotes	a
T1525	4904-4915	NN	denotes	consequence
T1526	4916-4918	IN	denotes	of
T1527	4919-4922	JJ	denotes	bad
T1529	4923-4926	NN	denotes	RNA
T1528	4927-4934	NN	denotes	quality
T1530	4935-4936	-LRB-	denotes	(
T1532	4936-4940	NNS	denotes	data
T1533	4941-4944	RB	denotes	not
T1531	4945-4950	VBN	denotes	shown
T1534	4950-4951	-RRB-	denotes	)
T1535	4951-4952	.	denotes	.
T1536	4952-5181	sentence	denotes	The ubiquitous expression pattern may be taken as another indication of the functional importance of Acdp proteins in fundamental biological processes in addition to the sequence conservation in evolutionarily divergent species.
T1537	4953-4956	DT	denotes	The
T1539	4957-4967	JJ	denotes	ubiquitous
T1540	4968-4978	NN	denotes	expression
T1538	4979-4986	NN	denotes	pattern
T1542	4987-4990	MD	denotes	may
T1543	4991-4993	VB	denotes	be
T1541	4994-4999	VBN	denotes	taken
T1544	5000-5002	IN	denotes	as
T1545	5003-5010	DT	denotes	another
T1546	5011-5021	NN	denotes	indication
T1547	5022-5024	IN	denotes	of
T1548	5025-5028	DT	denotes	the
T1550	5029-5039	JJ	denotes	functional
T1549	5040-5050	NN	denotes	importance
T1551	5051-5053	IN	denotes	of
T1552	5054-5058	NN	denotes	Acdp
T1553	5059-5067	NN	denotes	proteins
T1554	5068-5070	IN	denotes	in
T1555	5071-5082	JJ	denotes	fundamental
T1557	5083-5093	JJ	denotes	biological
T1556	5094-5103	NNS	denotes	processes
T1558	5104-5106	IN	denotes	in
T1559	5107-5115	NN	denotes	addition
T1560	5116-5118	IN	denotes	to
T1561	5119-5122	DT	denotes	the
T1563	5123-5131	NN	denotes	sequence
T1562	5132-5144	NN	denotes	conservation
T1564	5145-5147	IN	denotes	in
T1565	5148-5162	RB	denotes	evolutionarily
T1566	5163-5172	JJ	denotes	divergent
T1567	5173-5180	NNS	denotes	species
T1568	5180-5181	.	denotes	.
T5731	5192-5200	NNP	denotes	Northern
T5732	5201-5205	NN	denotes	blot
T5733	5206-5214	NNS	denotes	analyses
T5734	5215-5217	IN	denotes	of
T5735	5218-5221	DT	denotes	the
T5737	5222-5226	NN	denotes	Acdp
T5738	5227-5231	NN	denotes	gene
T5736	5232-5238	NN	denotes	family
T5739	5238-5239	.	denotes	.
T5740	5239-5281	sentence	denotes	S. muscle represents skeletal muscle, Sm.
T5741	5240-5242	NN	denotes	S.
T5742	5243-5249	NN	denotes	muscle
T5743	5250-5260	VBZ	denotes	represents
T5744	5261-5269	JJ	denotes	skeletal
T5745	5270-5276	NN	denotes	muscle
T5746	5276-5278	,	denotes	,
T5747	5278-5280	NN	denotes	Sm
T5748	5280-5281	.	denotes	.
T5749	5281-5314	sentence	denotes	Int. represents small intestine.
T5750	5282-5286	NN	denotes	Int.
T5751	5287-5297	VBZ	denotes	represents
T5752	5298-5303	JJ	denotes	small
T5753	5304-5313	NN	denotes	intestine
T5754	5313-5314	.	denotes	.
T5755	5314-5381	sentence	denotes	Multiple Choice Northern Blot filters were purchased from Origene.
T5756	5315-5323	JJ	denotes	Multiple
T5757	5324-5330	NN	denotes	Choice
T5759	5331-5339	NNP	denotes	Northern
T5758	5340-5344	NN	denotes	Blot
T5760	5345-5352	NNS	denotes	filters
T5762	5353-5357	VBD	denotes	were
T5761	5358-5367	VBN	denotes	purchased
T5763	5368-5372	IN	denotes	from
T5764	5373-5380	NNP	denotes	Origene
T5765	5380-5381	.	denotes	.
T1609	5383-5394	JJ	denotes	Chromosomal
T1610	5395-5403	NN	denotes	location
T1611	5403-5556	sentence	denotes	Radiation hybrid mapping indicated that the Acdp1 gene maps to chromosome 19 between markers D19Mit119 (34.3 cR proximal)and D19Mit112 (13.6 cR distal).
T1612	5404-5413	NN	denotes	Radiation
T1614	5414-5420	NN	denotes	hybrid
T1613	5421-5428	NN	denotes	mapping
T1615	5429-5438	VBD	denotes	indicated
T1616	5439-5443	IN	denotes	that
T1618	5444-5447	DT	denotes	the
T1620	5448-5453	NN	denotes	Acdp1
T1619	5454-5458	NN	denotes	gene
T1617	5459-5463	VBZ	denotes	maps
T1621	5464-5466	IN	denotes	to
T1622	5467-5477	NN	denotes	chromosome
T1623	5478-5480	CD	denotes	19
T1624	5481-5488	IN	denotes	between
T1625	5489-5496	NNS	denotes	markers
T1626	5497-5506	NN	denotes	D19Mit119
T1627	5507-5508	-LRB-	denotes	(
T1629	5508-5512	CD	denotes	34.3
T1628	5513-5515	NN	denotes	cR
T1630	5516-5524	JJ	denotes	proximal
T1631	5524-5525	-RRB-	denotes	)
T1632	5525-5528	CC	denotes	and
T1633	5529-5538	NN	denotes	D19Mit112
T1634	5539-5540	-LRB-	denotes	(
T1636	5540-5544	CD	denotes	13.6
T1635	5545-5547	NN	denotes	cR
T1637	5548-5554	JJ	denotes	distal
T1638	5554-5555	-RRB-	denotes	)
T1639	5555-5556	.	denotes	.
T1640	5556-5692	sentence	denotes	The Acdp2 gene maps slightly more distal to the Acdp1 on chromosome 19 between D19Mit9 (2.4 cR proximal) and D19Mit38 (15.1 cR distal).
T1641	5557-5560	DT	denotes	The
T1643	5561-5566	NN	denotes	Acdp2
T1642	5567-5571	NN	denotes	gene
T1644	5572-5576	VBZ	denotes	maps
T1645	5577-5585	RB	denotes	slightly
T1646	5586-5590	RBR	denotes	more
T1647	5591-5597	JJ	denotes	distal
T1648	5598-5600	IN	denotes	to
T1649	5601-5604	DT	denotes	the
T1650	5605-5610	NN	denotes	Acdp1
T1651	5611-5613	IN	denotes	on
T1652	5614-5624	NN	denotes	chromosome
T1653	5625-5627	CD	denotes	19
T1654	5628-5635	IN	denotes	between
T1655	5636-5643	NN	denotes	D19Mit9
T1656	5644-5645	-LRB-	denotes	(
T1658	5645-5648	CD	denotes	2.4
T1657	5649-5651	NN	denotes	cR
T1659	5652-5660	JJ	denotes	proximal
T1660	5660-5661	-RRB-	denotes	)
T1661	5662-5665	CC	denotes	and
T1662	5666-5674	NN	denotes	D19Mit38
T1663	5675-5676	-LRB-	denotes	(
T1665	5676-5680	CD	denotes	15.1
T1664	5681-5683	NN	denotes	cR
T1666	5684-5690	JJ	denotes	distal
T1667	5690-5691	-RRB-	denotes	)
T1668	5691-5692	.	denotes	.
T1669	5692-5813	sentence	denotes	The Acdp3 and Acdp4 genes map to chromosome 1 within one BAC clone (RP23-294I17), proximal to marker D1Mit171 (17.4 cR).
T1670	5693-5696	DT	denotes	The
T1672	5697-5702	NN	denotes	Acdp3
T1673	5703-5706	CC	denotes	and
T1674	5707-5712	NN	denotes	Acdp4
T1671	5713-5718	NNS	denotes	genes
T1675	5719-5722	VBP	denotes	map
T1676	5723-5725	IN	denotes	to
T1677	5726-5736	NN	denotes	chromosome
T1678	5737-5738	CD	denotes	1
T1679	5739-5745	IN	denotes	within
T1680	5746-5749	CD	denotes	one
T1682	5750-5753	NN	denotes	BAC
T1681	5754-5759	NN	denotes	clone
T1683	5760-5761	-LRB-	denotes	(
T1685	5761-5765	NN	denotes	RP23
T1686	5765-5766	HYPH	denotes	-
T1684	5766-5772	NN	denotes	294I17
T1687	5772-5773	-RRB-	denotes	)
T1688	5773-5775	,	denotes	,
T1689	5775-5783	JJ	denotes	proximal
T1690	5784-5786	IN	denotes	to
T1691	5787-5793	NN	denotes	marker
T1692	5794-5802	NN	denotes	D1Mit171
T1693	5803-5804	-LRB-	denotes	(
T1695	5804-5808	CD	denotes	17.4
T1694	5809-5811	NN	denotes	cR
T1696	5811-5812	-RRB-	denotes	)
T1697	5812-5813	.	denotes	.
T1698	5813-5867	sentence	denotes	These regions are syntenic to the human counterparts.
T1699	5814-5819	DT	denotes	These
T1700	5820-5827	NNS	denotes	regions
T1701	5828-5831	VBP	denotes	are
T1702	5832-5840	JJ	denotes	syntenic
T1703	5841-5843	IN	denotes	to
T1704	5844-5847	DT	denotes	the
T1706	5848-5853	JJ	denotes	human
T1705	5854-5866	NNS	denotes	counterparts
T1707	5866-5867	.	denotes	.
T2019	5869-5877	NN	denotes	Sequence
T2020	5878-5886	NN	denotes	homology
T2021	5887-5890	CC	denotes	and
T2022	5891-5900	JJ	denotes	molecular
T2023	5901-5916	NNS	denotes	characteristics
T2024	5916-6038	sentence	denotes	The mouse Acdp genes showed very strong homologies of both nucleotide and AA sequences to the human ACDP genes (Table 1).
T2025	5917-5920	DT	denotes	The
T2027	5921-5926	NN	denotes	mouse
T2028	5927-5931	NN	denotes	Acdp
T2026	5932-5937	NNS	denotes	genes
T2029	5938-5944	VBD	denotes	showed
T2030	5945-5949	RB	denotes	very
T2031	5950-5956	JJ	denotes	strong
T2032	5957-5967	NNS	denotes	homologies
T2033	5968-5970	IN	denotes	of
T2034	5971-5975	CC	denotes	both
T2035	5976-5986	NN	denotes	nucleotide
T2037	5987-5990	CC	denotes	and
T2038	5991-5993	NN	denotes	AA
T2036	5994-6003	NNS	denotes	sequences
T2039	6004-6006	IN	denotes	to
T2040	6007-6010	DT	denotes	the
T2042	6011-6016	JJ	denotes	human
T2043	6017-6021	NN	denotes	ACDP
T2041	6022-6027	NNS	denotes	genes
T2044	6028-6029	-LRB-	denotes	(
T2045	6029-6034	NN	denotes	Table
T2046	6035-6036	CD	denotes	1
T2047	6036-6037	-RRB-	denotes	)
T2048	6037-6038	.	denotes	.
T2049	6038-6199	sentence	denotes	The highest homologies were observed between the human ACDP2 and the mouse Acdp2 gene (91% of nucleotide identity, 97% of AA identity and 99.4% of AA homology).
T2050	6039-6042	DT	denotes	The
T2052	6043-6050	JJS	denotes	highest
T2051	6051-6061	NNS	denotes	homologies
T2054	6062-6066	VBD	denotes	were
T2053	6067-6075	VBN	denotes	observed
T2055	6076-6083	IN	denotes	between
T2056	6084-6087	DT	denotes	the
T2058	6088-6093	JJ	denotes	human
T2057	6094-6099	NN	denotes	ACDP2
T2059	6100-6103	CC	denotes	and
T2060	6104-6107	DT	denotes	the
T2062	6108-6113	NN	denotes	mouse
T2061	6114-6119	NN	denotes	Acdp2
T2063	6120-6124	NN	denotes	gene
T2064	6125-6126	-LRB-	denotes	(
T2066	6126-6128	CD	denotes	91
T2065	6128-6129	NN	denotes	%
T2067	6130-6132	IN	denotes	of
T2068	6133-6143	NN	denotes	nucleotide
T2069	6144-6152	NN	denotes	identity
T2070	6152-6154	,	denotes	,
T2071	6154-6156	CD	denotes	97
T2072	6156-6157	NN	denotes	%
T2073	6158-6160	IN	denotes	of
T2074	6161-6163	NN	denotes	AA
T2075	6164-6172	NN	denotes	identity
T2076	6173-6176	CC	denotes	and
T2077	6177-6181	CD	denotes	99.4
T2078	6181-6182	NN	denotes	%
T2079	6183-6185	IN	denotes	of
T2080	6186-6188	NN	denotes	AA
T2081	6189-6197	NN	denotes	homology
T2082	6197-6198	-RRB-	denotes	)
T2083	6198-6199	.	denotes	.
T2084	6199-6420	sentence	denotes	In addition, the 5' UTR nucleotide sequences (20 bp of nucleotides before start codon) also showed high homologies to the human homologs, for example, the Acdp2 5' UTR sequence showed 95% identities to its human homolog.
T2085	6200-6202	IN	denotes	In
T2087	6203-6211	NN	denotes	addition
T2088	6211-6213	,	denotes	,
T2089	6213-6216	DT	denotes	the
T2091	6217-6218	CD	denotes	5
T2093	6218-6219	SYM	denotes	'
T2092	6220-6223	NN	denotes	UTR
T2094	6224-6234	NN	denotes	nucleotide
T2090	6235-6244	NNS	denotes	sequences
T2095	6245-6246	-LRB-	denotes	(
T2097	6246-6248	CD	denotes	20
T2096	6249-6251	NNS	denotes	bp
T2098	6252-6254	IN	denotes	of
T2099	6255-6266	NNS	denotes	nucleotides
T2100	6267-6273	IN	denotes	before
T2101	6274-6279	NN	denotes	start
T2102	6280-6285	NN	denotes	codon
T2103	6285-6286	-RRB-	denotes	)
T2104	6287-6291	RB	denotes	also
T2086	6292-6298	VBD	denotes	showed
T2106	6299-6303	JJ	denotes	high
T2107	6304-6314	NNS	denotes	homologies
T2108	6315-6317	IN	denotes	to
T2109	6318-6321	DT	denotes	the
T2111	6322-6327	JJ	denotes	human
T2110	6328-6336	NNS	denotes	homologs
T2112	6336-6338	,	denotes	,
T2113	6338-6341	IN	denotes	for
T2114	6342-6349	NN	denotes	example
T2115	6349-6351	,	denotes	,
T2116	6351-6354	DT	denotes	the
T2118	6355-6360	NN	denotes	Acdp2
T2119	6361-6362	CD	denotes	5
T2121	6362-6363	SYM	denotes	'
T2120	6364-6367	NN	denotes	UTR
T2117	6368-6376	NN	denotes	sequence
T2105	6377-6383	VBD	denotes	showed
T2122	6384-6386	CD	denotes	95
T2123	6386-6387	NN	denotes	%
T2124	6388-6398	NNS	denotes	identities
T2125	6399-6401	IN	denotes	to
T2126	6402-6405	PRP$	denotes	its
T2128	6406-6411	JJ	denotes	human
T2127	6412-6419	NN	denotes	homolog
T2129	6419-6420	.	denotes	.
T2130	6420-6602	sentence	denotes	However, the homologies in the 3' UTR sequences (20 bp of nucleotides after stop codon) were much lower (40–55%) for all Acdp genes except Acdp4 (90% identity to its human homolog).
T2131	6421-6428	RB	denotes	However
T2133	6428-6430	,	denotes	,
T2134	6430-6433	DT	denotes	the
T2135	6434-6444	NNS	denotes	homologies
T2136	6445-6447	IN	denotes	in
T2137	6448-6451	DT	denotes	the
T2139	6452-6453	CD	denotes	3
T2141	6453-6454	SYM	denotes	'
T2140	6455-6458	NN	denotes	UTR
T2138	6459-6468	NNS	denotes	sequences
T2142	6469-6470	-LRB-	denotes	(
T2144	6470-6472	CD	denotes	20
T2143	6473-6475	NNS	denotes	bp
T2145	6476-6478	IN	denotes	of
T2146	6479-6490	NNS	denotes	nucleotides
T2147	6491-6496	IN	denotes	after
T2148	6497-6501	NN	denotes	stop
T2149	6502-6507	NN	denotes	codon
T2150	6507-6508	-RRB-	denotes	)
T2132	6509-6513	VBD	denotes	were
T2151	6514-6518	RB	denotes	much
T2152	6519-6524	JJR	denotes	lower
T2153	6525-6526	-LRB-	denotes	(
T2155	6526-6528	CD	denotes	40
T2157	6528-6529	SYM	denotes	–
T2156	6529-6531	CD	denotes	55
T2154	6531-6532	NN	denotes	%
T2158	6532-6533	-RRB-	denotes	)
T2159	6534-6537	IN	denotes	for
T2160	6538-6541	DT	denotes	all
T2162	6542-6546	NN	denotes	Acdp
T2161	6547-6552	NNS	denotes	genes
T2163	6553-6559	IN	denotes	except
T2164	6560-6565	NN	denotes	Acdp4
T2165	6566-6567	-LRB-	denotes	(
T2167	6567-6569	CD	denotes	90
T2168	6569-6570	NN	denotes	%
T2166	6571-6579	NN	denotes	identity
T2169	6580-6582	IN	denotes	to
T2170	6583-6586	PRP$	denotes	its
T2172	6587-6592	JJ	denotes	human
T2171	6593-6600	NN	denotes	homolog
T2173	6600-6601	-RRB-	denotes	)
T2174	6601-6602	.	denotes	.
T2175	6602-6736	sentence	denotes	The ancient conserved domain (ACD) has 55.3% of AA identity and 83.3% of homology between all mouse and human ACDP proteins (Fig. 2).
T2176	6603-6606	DT	denotes	The
T2178	6607-6614	JJ	denotes	ancient
T2179	6615-6624	VBN	denotes	conserved
T2177	6625-6631	NN	denotes	domain
T2181	6632-6633	-LRB-	denotes	(
T2182	6633-6636	NN	denotes	ACD
T2183	6636-6637	-RRB-	denotes	)
T2180	6638-6641	VBZ	denotes	has
T2184	6642-6646	CD	denotes	55.3
T2185	6646-6647	NN	denotes	%
T2186	6648-6650	IN	denotes	of
T2187	6651-6653	NN	denotes	AA
T2188	6654-6662	NN	denotes	identity
T2189	6663-6666	CC	denotes	and
T2190	6667-6671	CD	denotes	83.3
T2191	6671-6672	NN	denotes	%
T2192	6673-6675	IN	denotes	of
T2193	6676-6684	NN	denotes	homology
T2194	6685-6692	IN	denotes	between
T2195	6693-6696	DT	denotes	all
T2197	6697-6702	NN	denotes	mouse
T2198	6703-6706	CC	denotes	and
T2199	6707-6712	JJ	denotes	human
T2200	6713-6717	NN	denotes	ACDP
T2196	6718-6726	NN	denotes	proteins
T2201	6727-6728	-LRB-	denotes	(
T2202	6728-6732	NN	denotes	Fig.
T2203	6733-6734	CD	denotes	2
T2204	6734-6735	-RRB-	denotes	)
T2205	6735-6736	.	denotes	.
T2206	6736-6884	sentence	denotes	The ACD domain is evolutionarily conserved in divergent species ranging from bacteria, yeast, C. elegans, D. melanogaster, mouse to human (Fig. 3).
T2207	6737-6740	DT	denotes	The
T2209	6741-6744	NN	denotes	ACD
T2208	6745-6751	NN	denotes	domain
T2211	6752-6754	VBZ	denotes	is
T2212	6755-6769	RB	denotes	evolutionarily
T2210	6770-6779	VBN	denotes	conserved
T2213	6780-6782	IN	denotes	in
T2214	6783-6792	JJ	denotes	divergent
T2215	6793-6800	NNS	denotes	species
T2216	6801-6808	VBG	denotes	ranging
T2217	6809-6813	IN	denotes	from
T2218	6814-6822	NNS	denotes	bacteria
T2219	6822-6824	,	denotes	,
T2220	6824-6829	NN	denotes	yeast
T2221	6829-6831	,	denotes	,
T2222	6831-6833	NNP	denotes	C.
T2223	6834-6841	NNP	denotes	elegans
T2224	6841-6843	,	denotes	,
T2225	6843-6845	NNP	denotes	D.
T2226	6846-6858	NNP	denotes	melanogaster
T2227	6858-6860	,	denotes	,
T2228	6860-6865	NN	denotes	mouse
T2229	6866-6868	IN	denotes	to
T2230	6869-6874	JJ	denotes	human
T2231	6875-6876	-LRB-	denotes	(
T2232	6876-6880	NN	denotes	Fig.
T2233	6881-6882	CD	denotes	3
T2234	6882-6883	-RRB-	denotes	)
T2235	6883-6884	.	denotes	.
T2236	6884-7079	sentence	denotes	Particularly, as shown in Fig. 3, Acdp proteins showed very strong AA homology to bacteria CorC protein (35% AA identity with 55% homology), which is involved in magnesium and cobalt efflux [7].
T2237	6885-6897	RB	denotes	Particularly
T2239	6897-6899	,	denotes	,
T2240	6899-6901	IN	denotes	as
T2241	6902-6907	VBN	denotes	shown
T2242	6908-6910	IN	denotes	in
T2243	6911-6915	NN	denotes	Fig.
T2244	6916-6917	CD	denotes	3
T2245	6917-6919	,	denotes	,
T2246	6919-6923	NN	denotes	Acdp
T2247	6924-6932	NN	denotes	proteins
T2238	6933-6939	VBD	denotes	showed
T2248	6940-6944	RB	denotes	very
T2249	6945-6951	JJ	denotes	strong
T2251	6952-6954	NN	denotes	AA
T2250	6955-6963	NN	denotes	homology
T2252	6964-6966	IN	denotes	to
T2253	6967-6975	NNS	denotes	bacteria
T2255	6976-6980	NN	denotes	CorC
T2254	6981-6988	NN	denotes	protein
T2256	6989-6990	-LRB-	denotes	(
T2258	6990-6992	CD	denotes	35
T2259	6992-6993	NN	denotes	%
T2260	6994-6996	NN	denotes	AA
T2257	6997-7005	NN	denotes	identity
T2261	7006-7010	IN	denotes	with
T2262	7011-7013	CD	denotes	55
T2263	7013-7014	NN	denotes	%
T2264	7015-7023	NN	denotes	homology
T2265	7023-7024	-RRB-	denotes	)
T2266	7024-7026	,	denotes	,
T2267	7026-7031	WDT	denotes	which
T2269	7032-7034	VBZ	denotes	is
T2268	7035-7043	VBN	denotes	involved
T2270	7044-7046	IN	denotes	in
T2271	7047-7056	NN	denotes	magnesium
T2273	7057-7060	CC	denotes	and
T2274	7061-7067	NN	denotes	cobalt
T2272	7068-7074	NN	denotes	efflux
T2275	7075-7076	-LRB-	denotes	[
T2276	7076-7077	CD	denotes	7
T2277	7077-7078	-RRB-	denotes	]
T2278	7078-7079	.	denotes	.
T2279	7079-7205	sentence	denotes	High AA homology was also observed between the Acdp proteins and the yeast Amip3 protein (35% AA identity with 56% homology).
T2280	7080-7084	JJ	denotes	High
T2282	7085-7087	NN	denotes	AA
T2281	7088-7096	NN	denotes	homology
T2284	7097-7100	VBD	denotes	was
T2285	7101-7105	RB	denotes	also
T2283	7106-7114	VBN	denotes	observed
T2286	7115-7122	IN	denotes	between
T2287	7123-7126	DT	denotes	the
T2289	7127-7131	NN	denotes	Acdp
T2288	7132-7140	NN	denotes	proteins
T2290	7141-7144	CC	denotes	and
T2291	7145-7148	DT	denotes	the
T2293	7149-7154	NN	denotes	yeast
T2294	7155-7160	NN	denotes	Amip3
T2292	7161-7168	NN	denotes	protein
T2295	7169-7170	-LRB-	denotes	(
T2297	7170-7172	CD	denotes	35
T2298	7172-7173	NN	denotes	%
T2299	7174-7176	NN	denotes	AA
T2296	7177-7185	NN	denotes	identity
T2300	7186-7190	IN	denotes	with
T2301	7191-7193	CD	denotes	56
T2303	7193-7194	NN	denotes	%
T2302	7195-7203	NN	denotes	homology
T2304	7203-7204	-RRB-	denotes	)
T2305	7204-7205	.	denotes	.
T2306	7205-7274	sentence	denotes	The Amip3 is likely to be a homologous to the bacteria CorC protein.
T2307	7206-7209	DT	denotes	The
T2308	7210-7215	NN	denotes	Amip3
T2309	7216-7218	VBZ	denotes	is
T2310	7219-7225	JJ	denotes	likely
T2311	7226-7228	TO	denotes	to
T2312	7229-7231	VB	denotes	be
T2313	7232-7233	DT	denotes	a
T2314	7234-7244	JJ	denotes	homologous
T2315	7245-7247	IN	denotes	to
T2316	7248-7251	DT	denotes	the
T2318	7252-7260	NNS	denotes	bacteria
T2319	7261-7265	NN	denotes	CorC
T2317	7266-7273	NN	denotes	protein
T2320	7273-7274	.	denotes	.
T2321	7274-7426	sentence	denotes	The Amip3 mutants confer resistance to copper toxicity (Personal communication with Dr. V.C. Culotte, John Hopkins Bloomberg, School of Public Health).
T2322	7275-7278	DT	denotes	The
T2324	7279-7284	NN	denotes	Amip3
T2323	7285-7292	NNS	denotes	mutants
T2325	7293-7299	VBP	denotes	confer
T2326	7300-7310	NN	denotes	resistance
T2327	7311-7313	IN	denotes	to
T2328	7314-7320	NN	denotes	copper
T2329	7321-7329	NN	denotes	toxicity
T2330	7330-7331	-LRB-	denotes	(
T2332	7331-7339	JJ	denotes	Personal
T2333	7340-7353	NN	denotes	communication
T2334	7354-7358	IN	denotes	with
T2331	7359-7362	NNP	denotes	Dr.
T2335	7363-7367	NNP	denotes	V.C.
T2336	7368-7375	NNP	denotes	Culotte
T2337	7375-7377	,	denotes	,
T2338	7377-7381	NNP	denotes	John
T2339	7382-7389	NNP	denotes	Hopkins
T2340	7390-7399	NNP	denotes	Bloomberg
T2341	7399-7401	,	denotes	,
T2342	7401-7407	NNP	denotes	School
T2343	7408-7410	IN	denotes	of
T2344	7411-7417	NNP	denotes	Public
T2345	7418-7424	NNP	denotes	Health
T2346	7424-7425	-RRB-	denotes	)
T2347	7425-7426	.	denotes	.
T2348	7426-7576	sentence	denotes	The evolutionary relationships among those proteins are illustrated by a phylogenetic tree constructed based on the AA homology of proteins (Fig. 4).
T2349	7427-7430	DT	denotes	The
T2351	7431-7443	JJ	denotes	evolutionary
T2350	7444-7457	NNS	denotes	relationships
T2353	7458-7463	IN	denotes	among
T2354	7464-7469	DT	denotes	those
T2355	7470-7478	NN	denotes	proteins
T2356	7479-7482	VBP	denotes	are
T2352	7483-7494	VBN	denotes	illustrated
T2357	7495-7497	IN	denotes	by
T2358	7498-7499	DT	denotes	a
T2360	7500-7512	JJ	denotes	phylogenetic
T2359	7513-7517	NN	denotes	tree
T2361	7518-7529	VBN	denotes	constructed
T2362	7530-7535	VBN	denotes	based
T2363	7536-7538	IN	denotes	on
T2364	7539-7542	DT	denotes	the
T2366	7543-7545	NN	denotes	AA
T2365	7546-7554	NN	denotes	homology
T2367	7555-7557	IN	denotes	of
T2368	7558-7566	NN	denotes	proteins
T2369	7567-7568	-LRB-	denotes	(
T2370	7568-7572	NN	denotes	Fig.
T2371	7573-7574	CD	denotes	4
T2372	7574-7575	-RRB-	denotes	)
T2373	7575-7576	.	denotes	.
T2374	7576-7577	sentence	denotes
T6623	7586-7596	NN	denotes	Nucleotide
T6625	7597-7600	CC	denotes	and
T6626	7601-7606	NN	denotes	amino
T6627	7607-7611	NN	denotes	acid
T6624	7612-7622	NNS	denotes	homologies
T6628	7623-7624	-LRB-	denotes	(
T6629	7624-7625	NN	denotes	%
T6630	7625-7626	-RRB-	denotes	)
T6631	7627-7634	IN	denotes	between
T6632	7635-7640	JJ	denotes	human
T6633	7641-7645	NN	denotes	ACDP
T6635	7646-7649	CC	denotes	and
T6636	7650-7655	NN	denotes	mouse
T6637	7656-7660	NN	denotes	Acdp
T6634	7661-7668	NNS	denotes	members
T6638	7668-7669	.	denotes	.
T5826	7856-7861	NN	denotes	Amino
T5827	7862-7866	NN	denotes	acid
T5828	7867-7875	NN	denotes	sequence
T5830	7876-7884	NN	denotes	homology
T5829	7885-7894	NN	denotes	alignment
T5831	7895-7898	IN	denotes	for
T5832	7899-7902	DT	denotes	all
T5833	7903-7905	IN	denotes	of
T5834	7906-7909	DT	denotes	the
T5836	7910-7914	NN	denotes	ACDP
T5837	7915-7918	CC	denotes	and
T5838	7919-7923	NN	denotes	Acdp
T5835	7924-7929	NNS	denotes	genes
T5839	7930-7936	IN	denotes	within
T5840	7937-7940	DT	denotes	the
T5842	7941-7944	NN	denotes	ACD
T5841	7945-7951	NN	denotes	domain
T5843	7951-7952	.	denotes	.
T5844	7952-8118	sentence	denotes	The sequence data for the Acdp genes have been deposited in GenBank under accession number AF202994 (Acdp1), AF216961 (Acdp2), AF216964 (Acdp3) and AF216963 (Acdp4).
T5845	7953-7956	DT	denotes	The
T5847	7957-7965	NN	denotes	sequence
T5846	7966-7970	NNS	denotes	data
T5849	7971-7974	IN	denotes	for
T5850	7975-7978	DT	denotes	the
T5852	7979-7983	NN	denotes	Acdp
T5851	7984-7989	NNS	denotes	genes
T5853	7990-7994	VBP	denotes	have
T5854	7995-7999	VBN	denotes	been
T5848	8000-8009	VBN	denotes	deposited
T5855	8010-8012	IN	denotes	in
T5856	8013-8020	NNP	denotes	GenBank
T5857	8021-8026	IN	denotes	under
T5858	8027-8036	NN	denotes	accession
T5860	8037-8043	NN	denotes	number
T5859	8044-8052	NN	denotes	AF202994
T5861	8053-8054	-LRB-	denotes	(
T5862	8054-8059	NN	denotes	Acdp1
T5863	8059-8060	-RRB-	denotes	)
T5864	8060-8062	,	denotes	,
T5865	8062-8070	NN	denotes	AF216961
T5866	8071-8072	-LRB-	denotes	(
T5867	8072-8077	NN	denotes	Acdp2
T5868	8077-8078	-RRB-	denotes	)
T5869	8078-8080	,	denotes	,
T5870	8080-8088	NN	denotes	AF216964
T5871	8089-8090	-LRB-	denotes	(
T5872	8090-8095	NN	denotes	Acdp3
T5873	8095-8096	-RRB-	denotes	)
T5874	8097-8100	CC	denotes	and
T5875	8101-8109	NN	denotes	AF216963
T5876	8110-8111	-LRB-	denotes	(
T5877	8111-8116	NN	denotes	Acdp4
T5878	8116-8117	-RRB-	denotes	)
T5879	8117-8118	.	denotes	.
T5880	8118-8221	sentence	denotes	Identical amino acids or amino acids with very strong homologies among all proteins were shaded black.
T5881	8119-8128	JJ	denotes	Identical
T5883	8129-8134	NN	denotes	amino
T5882	8135-8140	NNS	denotes	acids
T5885	8141-8143	CC	denotes	or
T5886	8144-8149	NN	denotes	amino
T5887	8150-8155	NNS	denotes	acids
T5888	8156-8160	IN	denotes	with
T5889	8161-8165	RB	denotes	very
T5890	8166-8172	JJ	denotes	strong
T5891	8173-8183	NNS	denotes	homologies
T5892	8184-8189	IN	denotes	among
T5893	8190-8193	DT	denotes	all
T5894	8194-8202	NN	denotes	proteins
T5895	8203-8207	VBD	denotes	were
T5884	8208-8214	VBN	denotes	shaded
T5896	8215-8220	JJ	denotes	black
T5897	8220-8221	.	denotes	.
T5898	8221-8328	sentence	denotes	Identical amino acids or amino acids with very strong homologies in most of the proteins were shaded grey.
T5899	8222-8231	JJ	denotes	Identical
T5901	8232-8237	NN	denotes	amino
T5900	8238-8243	NNS	denotes	acids
T5903	8244-8246	CC	denotes	or
T5904	8247-8252	NN	denotes	amino
T5905	8253-8258	NNS	denotes	acids
T5906	8259-8263	IN	denotes	with
T5907	8264-8268	RB	denotes	very
T5908	8269-8275	JJ	denotes	strong
T5909	8276-8286	NNS	denotes	homologies
T5910	8287-8289	IN	denotes	in
T5911	8290-8294	JJS	denotes	most
T5912	8295-8297	IN	denotes	of
T5913	8298-8301	DT	denotes	the
T5914	8302-8310	NN	denotes	proteins
T5915	8311-8315	VBD	denotes	were
T5902	8316-8322	VBN	denotes	shaded
T5916	8323-8327	JJ	denotes	grey
T5917	8327-8328	.	denotes	.
T5918	8328-8372	sentence	denotes	Dot lines represent gaps for the alignment.
T5919	8329-8332	NN	denotes	Dot
T5920	8333-8338	NNS	denotes	lines
T5921	8339-8348	VBP	denotes	represent
T5922	8349-8353	NNS	denotes	gaps
T5923	8354-8357	IN	denotes	for
T5924	8358-8361	DT	denotes	the
T5925	8362-8371	NN	denotes	alignment
T5926	8371-8372	.	denotes	.
T6010	8383-8388	NN	denotes	Amino
T6011	8389-8393	NN	denotes	acid
T6013	8394-8402	NN	denotes	sequence
T6012	8403-8412	NN	denotes	alignment
T6014	8413-8420	VBG	denotes	showing
T6015	8421-8424	DT	denotes	the
T6016	8425-8437	NN	denotes	conservation
T6017	8438-8440	IN	denotes	of
T6018	8441-8444	NN	denotes	ACD
T6019	8445-8451	NN	denotes	domain
T6020	8452-8454	IN	denotes	in
T6021	8455-8462	JJ	denotes	various
T6022	8463-8470	NNS	denotes	species
T6023	8470-8471	.	denotes	.
T6024	8471-8533	sentence	denotes	Amip3 is a protein from Saccharomyces cerevisiae (NP_014581).
T6025	8472-8477	NN	denotes	Amip3
T6026	8478-8480	VBZ	denotes	is
T6027	8481-8482	DT	denotes	a
T6028	8483-8490	NN	denotes	protein
T6029	8491-8495	IN	denotes	from
T6030	8496-8509	NNP	denotes	Saccharomyces
T6031	8510-8520	NNP	denotes	cerevisiae
T6032	8521-8522	-LRB-	denotes	(
T6033	8522-8531	NN	denotes	NP_014581
T6034	8531-8532	-RRB-	denotes	)
T6035	8532-8533	.	denotes	.
T6036	8533-8585	sentence	denotes	CanG is a protein from Candida glabrata (AAF33142).
T6037	8534-8538	NN	denotes	CanG
T6038	8539-8541	VBZ	denotes	is
T6039	8542-8543	DT	denotes	a
T6040	8544-8551	NN	denotes	protein
T6041	8552-8556	IN	denotes	from
T6042	8557-8564	NNP	denotes	Candida
T6043	8565-8573	NNP	denotes	glabrata
T6044	8574-8575	-LRB-	denotes	(
T6045	8575-8583	NN	denotes	AAF33142
T6046	8583-8584	-RRB-	denotes	)
T6047	8584-8585	.	denotes	.
T6048	8585-8651	sentence	denotes	NeuC (EAA31204) is a hypothetical protein from Neurospora crassa.
T6049	8586-8590	NN	denotes	NeuC
T6051	8591-8592	-LRB-	denotes	(
T6052	8592-8600	NN	denotes	EAA31204
T6053	8600-8601	-RRB-	denotes	)
T6050	8602-8604	VBZ	denotes	is
T6054	8605-8606	DT	denotes	a
T6056	8607-8619	JJ	denotes	hypothetical
T6055	8620-8627	NN	denotes	protein
T6057	8628-8632	IN	denotes	from
T6058	8633-8643	NNP	denotes	Neurospora
T6059	8644-8650	NNP	denotes	crassa
T6060	8650-8651	.	denotes	.
T6061	8651-8696	sentence	denotes	DroM is a gene product from D. melanogaster.
T6062	8652-8656	NN	denotes	DroM
T6063	8657-8659	VBZ	denotes	is
T6064	8660-8661	DT	denotes	a
T6066	8662-8666	NN	denotes	gene
T6065	8667-8674	NN	denotes	product
T6067	8675-8679	IN	denotes	from
T6068	8680-8682	NNP	denotes	D.
T6069	8683-8695	NNP	denotes	melanogaster
T6070	8695-8696	.	denotes	.
T6071	8696-8788	sentence	denotes	The accession number for this gene is CG40084 in BDGP (Berkeley Drosophila Genome Project).
T6072	8697-8700	DT	denotes	The
T6074	8701-8710	NN	denotes	accession
T6073	8711-8717	NN	denotes	number
T6076	8718-8721	IN	denotes	for
T6077	8722-8726	DT	denotes	this
T6078	8727-8731	NN	denotes	gene
T6075	8732-8734	VBZ	denotes	is
T6079	8735-8742	NN	denotes	CG40084
T6080	8743-8745	IN	denotes	in
T6081	8746-8750	NN	denotes	BDGP
T6082	8751-8752	-LRB-	denotes	(
T6083	8752-8760	NNP	denotes	Berkeley
T6085	8761-8771	NNP	denotes	Drosophila
T6086	8772-8778	NNP	denotes	Genome
T6084	8779-8786	NNP	denotes	Project
T6087	8786-8787	-RRB-	denotes	)
T6088	8787-8788	.	denotes	.
T6089	8788-8858	sentence	denotes	AnoG represents a protein from the anopheles gambiae str. (EAA01004).
T6090	8789-8793	NN	denotes	AnoG
T6091	8794-8804	VBZ	denotes	represents
T6092	8805-8806	DT	denotes	a
T6093	8807-8814	NN	denotes	protein
T6094	8815-8819	IN	denotes	from
T6095	8820-8823	DT	denotes	the
T6097	8824-8833	NNP	denotes	anopheles
T6098	8834-8841	NNP	denotes	gambiae
T6096	8842-8846	NN	denotes	str.
T6099	8847-8848	-LRB-	denotes	(
T6100	8848-8856	NN	denotes	EAA01004
T6101	8856-8857	-RRB-	denotes	)
T6102	8857-8858	.	denotes	.
T6103	8858-8932	sentence	denotes	CaeE (AAK77203) is a hypothetical protein from the Caenohabditis elegans.
T6104	8859-8863	NN	denotes	CaeE
T6106	8864-8865	-LRB-	denotes	(
T6107	8865-8873	NN	denotes	AAK77203
T6108	8873-8874	-RRB-	denotes	)
T6105	8875-8877	VBZ	denotes	is
T6109	8878-8879	DT	denotes	a
T6111	8880-8892	JJ	denotes	hypothetical
T6110	8893-8900	NN	denotes	protein
T6112	8901-8905	IN	denotes	from
T6113	8906-8909	DT	denotes	the
T6115	8910-8923	NNP	denotes	Caenohabditis
T6114	8924-8931	NNP	denotes	elegans
T6116	8931-8932	.	denotes	.
T6117	8932-9025	sentence	denotes	CorC represents bacteria magnesium and cobalt efflux protein from the Shewanella oneidensis.
T6118	8933-8937	NN	denotes	CorC
T6119	8938-8948	VBZ	denotes	represents
T6120	8949-8957	NNS	denotes	bacteria
T6121	8958-8967	NN	denotes	magnesium
T6122	8968-8971	CC	denotes	and
T6123	8972-8978	NN	denotes	cobalt
T6125	8979-8985	NN	denotes	efflux
T6124	8986-8993	NN	denotes	protein
T6126	8994-8998	IN	denotes	from
T6127	8999-9002	DT	denotes	the
T6129	9003-9013	NNP	denotes	Shewanella
T6128	9014-9024	NNP	denotes	oneidensis
T6130	9024-9025	.	denotes	.
T6131	9025-9105	sentence	denotes	XyFD is a hypothetical protein from the Xylella fastidiosa Dixon (ZP_00038107).
T6132	9026-9030	NN	denotes	XyFD
T6133	9031-9033	VBZ	denotes	is
T6134	9034-9035	DT	denotes	a
T6136	9036-9048	JJ	denotes	hypothetical
T6135	9049-9056	NN	denotes	protein
T6137	9057-9061	IN	denotes	from
T6138	9062-9065	DT	denotes	the
T6140	9066-9073	NNP	denotes	Xylella
T6141	9074-9084	NNP	denotes	fastidiosa
T6139	9085-9090	NNP	denotes	Dixon
T6142	9091-9092	-LRB-	denotes	(
T6143	9092-9103	NN	denotes	ZP_00038107
T6144	9103-9104	-RRB-	denotes	)
T6145	9104-9105	.	denotes	.
T6153	9116-9128	JJ	denotes	Phylogenetic
T6154	9129-9133	NN	denotes	tree
T6155	9134-9141	VBG	denotes	showing
T6156	9142-9155	NNS	denotes	relationships
T6157	9156-9161	IN	denotes	among
T6158	9162-9170	NN	denotes	proteins
T6159	9171-9181	VBG	denotes	containing
T6160	9182-9185	DT	denotes	the
T6162	9186-9189	NN	denotes	ACD
T6161	9190-9196	NN	denotes	domain
T6163	9197-9201	IN	denotes	from
T6164	9202-9208	NN	denotes	figure
T6165	9209-9210	CD	denotes	2
T6166	9211-9214	CC	denotes	and
T6167	9215-9216	CD	denotes	3
T6168	9216-9217	.	denotes	.
T6169	9217-9330	sentence	denotes	The phylogenetic tree was constructed according to the calculation of the best match for the selected sequences.
T6170	9218-9221	DT	denotes	The
T6172	9222-9234	JJ	denotes	phylogenetic
T6171	9235-9239	NN	denotes	tree
T6174	9240-9243	VBD	denotes	was
T6173	9244-9255	VBN	denotes	constructed
T6175	9256-9265	VBG	denotes	according
T6176	9266-9268	IN	denotes	to
T6177	9269-9272	DT	denotes	the
T6178	9273-9284	NN	denotes	calculation
T6179	9285-9287	IN	denotes	of
T6180	9288-9291	DT	denotes	the
T6182	9292-9296	JJS	denotes	best
T6181	9297-9302	NN	denotes	match
T6183	9303-9306	IN	denotes	for
T6184	9307-9310	DT	denotes	the
T6186	9311-9319	JJ	denotes	selected
T6185	9320-9329	NNS	denotes	sequences
T6187	9329-9330	.	denotes	.
T6188	9330-9400	sentence	denotes	Abbreviations for each protein are the same as presented in figure 3.
T6189	9331-9344	NNS	denotes	Abbreviations
T6191	9345-9348	IN	denotes	for
T6192	9349-9353	DT	denotes	each
T6193	9354-9361	NN	denotes	protein
T6190	9362-9365	VBP	denotes	are
T6194	9366-9369	DT	denotes	the
T6195	9370-9374	JJ	denotes	same
T6196	9375-9377	IN	denotes	as
T6197	9378-9387	VBN	denotes	presented
T6198	9388-9390	IN	denotes	in
T6199	9391-9397	NN	denotes	figure
T6200	9398-9399	CD	denotes	3
T6201	9399-9400	.	denotes	.
T2376	9401-9403	PRP	denotes	We
T2375	9401-9584	sentence	denotes	We found that all mouse Acdp members contain four distinct transmembrane domains (Fig. 5), two CBS domains and a DUF21 domain that are found in bacteria CorC and yeast Amip3 proteins.
T2377	9404-9409	VBD	denotes	found
T2378	9410-9414	IN	denotes	that
T2380	9415-9418	DT	denotes	all
T2382	9419-9424	NN	denotes	mouse
T2383	9425-9429	NN	denotes	Acdp
T2381	9430-9437	NNS	denotes	members
T2379	9438-9445	VBP	denotes	contain
T2384	9446-9450	CD	denotes	four
T2386	9451-9459	JJ	denotes	distinct
T2387	9460-9473	NN	denotes	transmembrane
T2385	9474-9481	NNS	denotes	domains
T2388	9482-9483	-LRB-	denotes	(
T2389	9483-9487	NN	denotes	Fig.
T2390	9488-9489	CD	denotes	5
T2391	9489-9490	-RRB-	denotes	)
T2392	9490-9492	,	denotes	,
T2393	9492-9495	CD	denotes	two
T2395	9496-9499	NN	denotes	CBS
T2394	9500-9507	NNS	denotes	domains
T2396	9508-9511	CC	denotes	and
T2397	9512-9513	DT	denotes	a
T2399	9514-9519	NN	denotes	DUF21
T2398	9520-9526	NN	denotes	domain
T2400	9527-9531	WDT	denotes	that
T2402	9532-9535	VBP	denotes	are
T2401	9536-9541	VBN	denotes	found
T2403	9542-9544	IN	denotes	in
T2404	9545-9553	NNS	denotes	bacteria
T2405	9554-9558	NN	denotes	CorC
T2407	9559-9562	CC	denotes	and
T2408	9563-9568	NN	denotes	yeast
T2409	9569-9574	NN	denotes	Amip3
T2406	9575-9583	NN	denotes	proteins
T2410	9583-9584	.	denotes	.
T2411	9584-9688	sentence	denotes	CBS domains are small intracellular modules that are mostly found in 2 or four copies within a protein.
T2412	9585-9588	NN	denotes	CBS
T2413	9589-9596	NNS	denotes	domains
T2414	9597-9600	VBP	denotes	are
T2415	9601-9606	JJ	denotes	small
T2417	9607-9620	JJ	denotes	intracellular
T2416	9621-9628	NNS	denotes	modules
T2418	9629-9633	WDT	denotes	that
T2420	9634-9637	VBP	denotes	are
T2421	9638-9644	RB	denotes	mostly
T2419	9645-9650	VBN	denotes	found
T2422	9651-9653	IN	denotes	in
T2423	9654-9655	CD	denotes	2
T2425	9656-9658	CC	denotes	or
T2426	9659-9663	CD	denotes	four
T2424	9664-9670	NNS	denotes	copies
T2427	9671-9677	IN	denotes	within
T2428	9678-9679	DT	denotes	a
T2429	9680-9687	NN	denotes	protein
T2430	9687-9688	.	denotes	.
T2431	9688-9756	sentence	denotes	Pairs of CBS domains dimerise to form a stable globular domain [8].
T2432	9689-9694	NNS	denotes	Pairs
T2434	9695-9697	IN	denotes	of
T2435	9698-9701	NN	denotes	CBS
T2436	9702-9709	NNS	denotes	domains
T2433	9710-9718	VBP	denotes	dimerise
T2437	9719-9721	TO	denotes	to
T2438	9722-9726	VB	denotes	form
T2439	9727-9728	DT	denotes	a
T2441	9729-9735	JJ	denotes	stable
T2442	9736-9744	JJ	denotes	globular
T2440	9745-9751	NN	denotes	domain
T2443	9752-9753	-LRB-	denotes	[
T2444	9753-9754	CD	denotes	8
T2445	9754-9755	-RRB-	denotes	]
T2446	9755-9756	.	denotes	.
T2447	9756-9829	sentence	denotes	DUF21 (CD: pfam01959.9) is a newly defined domain with unknown function.
T2448	9757-9762	NN	denotes	DUF21
T2450	9763-9764	-LRB-	denotes	(
T2452	9764-9766	NN	denotes	CD
T2453	9766-9768	:	denotes	:
T2451	9768-9779	NN	denotes	pfam01959.9
T2454	9779-9780	-RRB-	denotes	)
T2449	9781-9783	VBZ	denotes	is
T2455	9784-9785	DT	denotes	a
T2457	9786-9791	RB	denotes	newly
T2458	9792-9799	VBN	denotes	defined
T2456	9800-9806	NN	denotes	domain
T2459	9807-9811	IN	denotes	with
T2460	9812-9819	JJ	denotes	unknown
T2461	9820-9828	NN	denotes	function
T2462	9828-9829	.	denotes	.
T2463	9829-9969	sentence	denotes	This domain is a transmembrane region and found to be located in the N-terminus of the proteins adjacent to two intracellular CBS domains .
T2464	9830-9834	DT	denotes	This
T2465	9835-9841	NN	denotes	domain
T2466	9842-9844	VBZ	denotes	is
T2467	9845-9846	DT	denotes	a
T2469	9847-9860	JJ	denotes	transmembrane
T2468	9861-9867	NN	denotes	region
T2470	9868-9871	CC	denotes	and
T2471	9872-9877	VBN	denotes	found
T2472	9878-9880	TO	denotes	to
T2474	9881-9883	VB	denotes	be
T2473	9884-9891	VBN	denotes	located
T2475	9892-9894	IN	denotes	in
T2476	9895-9898	DT	denotes	the
T2478	9899-9900	NN	denotes	N
T2479	9900-9901	HYPH	denotes	-
T2477	9901-9909	NN	denotes	terminus
T2480	9910-9912	IN	denotes	of
T2481	9913-9916	DT	denotes	the
T2482	9917-9925	NN	denotes	proteins
T2483	9926-9934	JJ	denotes	adjacent
T2484	9935-9937	IN	denotes	to
T2485	9938-9941	CD	denotes	two
T2487	9942-9955	JJ	denotes	intracellular
T2488	9956-9959	NN	denotes	CBS
T2486	9960-9967	NNS	denotes	domains
T2489	9968-9969	.	denotes	.
T2490	9969-10071	sentence	denotes	A cNMP-binding domain (cyclic nucleotide-monophosphate-binding domain) was found in all Acdp members.
T2491	9970-9971	DT	denotes	A
T2493	9972-9976	NN	denotes	cNMP
T2495	9976-9977	HYPH	denotes	-
T2494	9977-9984	VBG	denotes	binding
T2492	9985-9991	NN	denotes	domain
T2497	9992-9993	-LRB-	denotes	(
T2499	9993-9999	JJ	denotes	cyclic
T2501	10000-10010	NN	denotes	nucleotide
T2502	10010-10011	HYPH	denotes	-
T2500	10011-10024	NN	denotes	monophosphate
T2503	10024-10025	HYPH	denotes	-
T2504	10025-10032	VBG	denotes	binding
T2498	10033-10039	NN	denotes	domain
T2505	10039-10040	-RRB-	denotes	)
T2506	10041-10044	VBD	denotes	was
T2496	10045-10050	VBN	denotes	found
T2507	10051-10053	IN	denotes	in
T2508	10054-10057	DT	denotes	all
T2510	10058-10062	NN	denotes	Acdp
T2509	10063-10070	NNS	denotes	members
T2511	10070-10071	.	denotes	.
T2512	10071-10798	sentence	denotes	Figure 5 Four transmembrane domains within Acdp4 protein. Transmembrane domains were predicted by the TMHMM program . The plot shows the posterior probabilities of inside /outside/TM helix. At the top of the plot (between 1 and 1.2) the N-best prediction is shown. The plot is obtained by calculating the total probability that a residue sits in helix, inside, or outside summed over all possible paths through the model. In addition, Acdp1 contains an Alanine-rich region (2–10: AAAAAAAAA), a Leucine-rich region (204–257: LLRVRPRLYGPGGDLLPPAWLRALGALLLLALSALF SGLRLSLLSLDPVELRVL), a Proline-rich region (78–130: PGPPVPAAPVPAPSLA PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP), and two amidation sites (917–920: MGKK; 926–929: SGRK).
T6228	10082-10086	CD	denotes	Four
T6230	10087-10100	NN	denotes	transmembrane
T6229	10101-10108	NNS	denotes	domains
T6231	10109-10115	IN	denotes	within
T6232	10116-10121	NN	denotes	Acdp4
T6233	10122-10129	NN	denotes	protein
T6234	10129-10130	.	denotes	.
T6235	10130-10190	sentence	denotes	Transmembrane domains were predicted by the TMHMM program .
T6236	10131-10144	NN	denotes	Transmembrane
T6237	10145-10152	NNS	denotes	domains
T6239	10153-10157	VBD	denotes	were
T6238	10158-10167	VBN	denotes	predicted
T6240	10168-10170	IN	denotes	by
T6241	10171-10174	DT	denotes	the
T6243	10175-10180	NN	denotes	TMHMM
T6242	10181-10188	NN	denotes	program
T6244	10189-10190	.	denotes	.
T6245	10190-10262	sentence	denotes	The plot shows the posterior probabilities of inside /outside/TM helix.
T6246	10191-10194	DT	denotes	The
T6247	10195-10199	NN	denotes	plot
T6248	10200-10205	VBZ	denotes	shows
T6249	10206-10209	DT	denotes	the
T6251	10210-10219	JJ	denotes	posterior
T6250	10220-10233	NNS	denotes	probabilities
T6252	10234-10236	IN	denotes	of
T6253	10237-10243	JJ	denotes	inside
T6255	10244-10245	HYPH	denotes	/
T6256	10245-10252	JJ	denotes	outside
T6257	10252-10253	HYPH	denotes	/
T6254	10253-10255	NN	denotes	TM
T6258	10256-10261	NN	denotes	helix
T6259	10261-10262	.	denotes	.
T6260	10262-10337	sentence	denotes	At the top of the plot (between 1 and 1.2) the N-best prediction is shown.
T6261	10263-10265	IN	denotes	At
T6263	10266-10269	DT	denotes	the
T6264	10270-10273	NN	denotes	top
T6265	10274-10276	IN	denotes	of
T6266	10277-10280	DT	denotes	the
T6267	10281-10285	NN	denotes	plot
T6268	10286-10287	-LRB-	denotes	(
T6269	10287-10294	IN	denotes	between
T6270	10295-10296	CD	denotes	1
T6271	10297-10300	CC	denotes	and
T6272	10301-10304	CD	denotes	1.2
T6273	10304-10305	-RRB-	denotes	)
T6274	10306-10309	DT	denotes	the
T6276	10310-10311	NN	denotes	N
T6278	10311-10312	HYPH	denotes	-
T6277	10312-10316	JJS	denotes	best
T6275	10317-10327	NN	denotes	prediction
T6279	10328-10330	VBZ	denotes	is
T6262	10331-10336	VBN	denotes	shown
T6280	10336-10337	.	denotes	.
T6281	10337-10494	sentence	denotes	The plot is obtained by calculating the total probability that a residue sits in helix, inside, or outside summed over all possible paths through the model.
T6282	10338-10341	DT	denotes	The
T6283	10342-10346	NN	denotes	plot
T6285	10347-10349	VBZ	denotes	is
T6284	10350-10358	VBN	denotes	obtained
T6286	10359-10361	IN	denotes	by
T6287	10362-10373	VBG	denotes	calculating
T6288	10374-10377	DT	denotes	the
T6290	10378-10383	JJ	denotes	total
T6289	10384-10395	NN	denotes	probability
T6291	10396-10400	IN	denotes	that
T6293	10401-10402	DT	denotes	a
T6294	10403-10410	NN	denotes	residue
T6292	10411-10415	VBZ	denotes	sits
T6295	10416-10418	IN	denotes	in
T6296	10419-10424	NN	denotes	helix
T6297	10424-10426	,	denotes	,
T6298	10426-10432	RB	denotes	inside
T6299	10432-10434	,	denotes	,
T6300	10434-10436	CC	denotes	or
T6301	10437-10444	RB	denotes	outside
T6302	10445-10451	VBD	denotes	summed
T6303	10452-10456	IN	denotes	over
T6304	10457-10460	DT	denotes	all
T6306	10461-10469	JJ	denotes	possible
T6305	10470-10475	NNS	denotes	paths
T6307	10476-10483	IN	denotes	through
T6308	10484-10487	DT	denotes	the
T6309	10488-10493	NN	denotes	model
T6310	10493-10494	.	denotes	.
T2513	10495-10497	IN	denotes	In
T2515	10498-10506	NN	denotes	addition
T2516	10506-10508	,	denotes	,
T2517	10508-10513	NN	denotes	Acdp1
T2514	10514-10522	VBZ	denotes	contains
T2518	10523-10525	DT	denotes	an
T2520	10526-10533	NN	denotes	Alanine
T2522	10533-10534	HYPH	denotes	-
T2521	10534-10538	JJ	denotes	rich
T2519	10539-10545	NN	denotes	region
T2523	10546-10547	-LRB-	denotes	(
T2525	10547-10548	CD	denotes	2
T2526	10548-10549	SYM	denotes	–
T2527	10549-10551	CD	denotes	10
T2528	10551-10553	:	denotes	:
T2524	10553-10562	NN	denotes	AAAAAAAAA
T2529	10562-10563	-RRB-	denotes	)
T2530	10563-10565	,	denotes	,
T2531	10565-10566	DT	denotes	a
T2533	10567-10574	NN	denotes	Leucine
T2535	10574-10575	HYPH	denotes	-
T2534	10575-10579	JJ	denotes	rich
T2532	10580-10586	NN	denotes	region
T2536	10587-10588	-LRB-	denotes	(
T2538	10588-10591	CD	denotes	204
T2539	10591-10592	SYM	denotes	–
T2540	10592-10595	CD	denotes	257
T2541	10595-10597	:	denotes	:
T2542	10597-10633	NN	denotes	LLRVRPRLYGPGGDLLPPAWLRALGALLLLALSALF
T2537	10634-10652	NN	denotes	SGLRLSLLSLDPVELRVL
T2543	10652-10653	-RRB-	denotes	)
T2544	10653-10655	,	denotes	,
T2545	10655-10656	DT	denotes	a
T2547	10657-10664	NN	denotes	Proline
T2549	10664-10665	HYPH	denotes	-
T2548	10665-10669	JJ	denotes	rich
T2546	10670-10676	NN	denotes	region
T2550	10677-10678	-LRB-	denotes	(
T2552	10678-10680	CD	denotes	78
T2553	10680-10681	SYM	denotes	–
T2554	10681-10684	CD	denotes	130
T2555	10684-10686	:	denotes	:
T2556	10686-10702	NN	denotes	PGPPVPAAPVPAPSLA
T2551	10703-10740	NN	denotes	PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
T2557	10740-10741	-RRB-	denotes	)
T2558	10741-10743	,	denotes	,
T2559	10743-10746	CC	denotes	and
T2560	10747-10750	CD	denotes	two
T2562	10751-10760	NN	denotes	amidation
T2561	10761-10766	NNS	denotes	sites
T2563	10767-10768	-LRB-	denotes	(
T2565	10768-10771	CD	denotes	917
T2567	10771-10772	SYM	denotes	–
T2568	10772-10775	CD	denotes	920
T2569	10775-10777	:	denotes	:
T2566	10777-10781	NN	denotes	MGKK
T2570	10781-10782	:	denotes	;
T2571	10783-10786	CD	denotes	926
T2572	10786-10787	SYM	denotes	–
T2573	10787-10790	CD	denotes	929
T2574	10790-10792	:	denotes	:
T2564	10792-10796	NN	denotes	SGRK
T2575	10796-10797	-RRB-	denotes	)
T2576	10797-10798	.	denotes	.
T2577	10798-10865	sentence	denotes	Acdp2 has a glycine-rich region (201–222: GAGGSGSASGTVGGKGGAGVAG).
T2578	10799-10804	NN	denotes	Acdp2
T2579	10805-10808	VBZ	denotes	has
T2580	10809-10810	DT	denotes	a
T2582	10811-10818	NN	denotes	glycine
T2584	10818-10819	HYPH	denotes	-
T2583	10819-10823	JJ	denotes	rich
T2581	10824-10830	NN	denotes	region
T2585	10831-10832	-LRB-	denotes	(
T2587	10832-10835	CD	denotes	201
T2588	10835-10836	SYM	denotes	–
T2589	10836-10839	CD	denotes	222
T2590	10839-10841	:	denotes	:
T2586	10841-10863	NN	denotes	GAGGSGSASGTVGGKGGAGVAG
T2591	10863-10864	-RRB-	denotes	)
T2592	10864-10865	.	denotes	.
T2593	10865-10960	sentence	denotes	Acdp3 possesses a large alanine-rich region (2–261) and a large leucine-rich region (201–299).
T2594	10866-10871	NN	denotes	Acdp3
T2595	10872-10881	VBZ	denotes	possesses
T2596	10882-10883	DT	denotes	a
T2598	10884-10889	JJ	denotes	large
T2599	10890-10897	NN	denotes	alanine
T2601	10897-10898	HYPH	denotes	-
T2600	10898-10902	JJ	denotes	rich
T2597	10903-10909	NN	denotes	region
T2602	10910-10911	-LRB-	denotes	(
T2603	10911-10912	CD	denotes	2
T2604	10912-10913	SYM	denotes	–
T2605	10913-10916	CD	denotes	261
T2606	10916-10917	-RRB-	denotes	)
T2607	10918-10921	CC	denotes	and
T2608	10922-10923	DT	denotes	a
T2610	10924-10929	JJ	denotes	large
T2611	10930-10937	NN	denotes	leucine
T2613	10937-10938	HYPH	denotes	-
T2612	10938-10942	JJ	denotes	rich
T2609	10943-10949	NN	denotes	region
T2614	10950-10951	-LRB-	denotes	(
T2615	10951-10954	CD	denotes	201
T2616	10954-10955	SYM	denotes	–
T2617	10955-10958	CD	denotes	299
T2618	10958-10959	-RRB-	denotes	)
T2619	10959-10960	.	denotes	.
T2620	10960-11070	sentence	denotes	Acdp4 contains a leucine zipper pattern (185–206: LVMVLLVLSGIFSGLNLGLMAL) and an amidation site (7–10: GGRR).
T2621	10961-10966	NN	denotes	Acdp4
T2622	10967-10975	VBZ	denotes	contains
T2623	10976-10977	DT	denotes	a
T2625	10978-10985	NN	denotes	leucine
T2626	10986-10992	NN	denotes	zipper
T2624	10993-11000	NN	denotes	pattern
T2627	11001-11002	-LRB-	denotes	(
T2629	11002-11005	CD	denotes	185
T2630	11005-11006	SYM	denotes	–
T2631	11006-11009	CD	denotes	206
T2632	11009-11011	:	denotes	:
T2628	11011-11033	NN	denotes	LVMVLLVLSGIFSGLNLGLMAL
T2633	11033-11034	-RRB-	denotes	)
T2634	11035-11038	CC	denotes	and
T2635	11039-11041	DT	denotes	an
T2637	11042-11051	NN	denotes	amidation
T2636	11052-11056	NN	denotes	site
T2638	11057-11058	-LRB-	denotes	(
T2640	11058-11059	CD	denotes	7
T2641	11059-11060	SYM	denotes	–
T2642	11060-11062	CD	denotes	10
T2643	11062-11064	:	denotes	:
T2639	11064-11068	NN	denotes	GGRR
T2644	11068-11069	-RRB-	denotes	)
T2645	11069-11070	.	denotes	.
T2862	11072-11080	NN	denotes	Antibody
T2863	11081-11091	NN	denotes	production
T2864	11091-11093	,	denotes	,
T2865	11093-11100	NNP	denotes	Western
T2866	11101-11108	NNS	denotes	results
T2867	11109-11112	CC	denotes	and
T2868	11113-11124	JJ	denotes	subcellular
T2869	11125-11137	NN	denotes	localization
T2870	11137-11345	sentence	denotes	Peptides from Acdp1 N- (TSFLLRVYFQPGPPATAAPVPSPT) and C- (TQQLTLSPAAVPTR) terminuses, conserved peptide from ACD domain of Acdp1 (HNIVDILFVKDLAFVDPDDCTPLLTVTRF) were commercially synthesized (Sigma Genosys).
T2871	11138-11146	NNS	denotes	Peptides
T2873	11147-11151	IN	denotes	from
T2874	11152-11157	NN	denotes	Acdp1
T2876	11158-11159	NN	denotes	N
T2877	11159-11160	HYPH	denotes	-
T2878	11161-11162	-LRB-	denotes	(
T2879	11162-11186	NN	denotes	TSFLLRVYFQPGPPATAAPVPSPT
T2880	11186-11187	-RRB-	denotes	)
T2881	11188-11191	CC	denotes	and
T2882	11192-11193	NN	denotes	C
T2883	11193-11194	HYPH	denotes	-
T2884	11195-11196	-LRB-	denotes	(
T2885	11196-11210	NN	denotes	TQQLTLSPAAVPTR
T2886	11210-11211	-RRB-	denotes	)
T2875	11212-11222	NNS	denotes	terminuses
T2887	11222-11224	,	denotes	,
T2888	11224-11233	JJ	denotes	conserved
T2889	11234-11241	NN	denotes	peptide
T2890	11242-11246	IN	denotes	from
T2891	11247-11250	NN	denotes	ACD
T2892	11251-11257	NN	denotes	domain
T2893	11258-11260	IN	denotes	of
T2894	11261-11266	NN	denotes	Acdp1
T2895	11267-11268	-LRB-	denotes	(
T2896	11268-11297	NN	denotes	HNIVDILFVKDLAFVDPDDCTPLLTVTRF
T2897	11297-11298	-RRB-	denotes	)
T2898	11299-11303	VBD	denotes	were
T2899	11304-11316	RB	denotes	commercially
T2872	11317-11328	VBN	denotes	synthesized
T2900	11329-11330	-LRB-	denotes	(
T2902	11330-11335	NNP	denotes	Sigma
T2901	11336-11343	NNP	denotes	Genosys
T2903	11343-11344	-RRB-	denotes	)
T2904	11344-11345	.	denotes	.
T2905	11345-11493	sentence	denotes	These antigenic sites were predicted by software from Sigma Genosys and polyclonal antibodies for each peptide were produced by immunizing rabbits.
T2906	11346-11351	DT	denotes	These
T2908	11352-11361	JJ	denotes	antigenic
T2907	11362-11367	NNS	denotes	sites
T2910	11368-11372	VBD	denotes	were
T2909	11373-11382	VBN	denotes	predicted
T2911	11383-11385	IN	denotes	by
T2912	11386-11394	NN	denotes	software
T2913	11395-11399	IN	denotes	from
T2914	11400-11405	NNP	denotes	Sigma
T2915	11406-11413	NNP	denotes	Genosys
T2916	11414-11417	CC	denotes	and
T2917	11418-11428	JJ	denotes	polyclonal
T2918	11429-11439	NNS	denotes	antibodies
T2920	11440-11443	IN	denotes	for
T2921	11444-11448	DT	denotes	each
T2922	11449-11456	NN	denotes	peptide
T2923	11457-11461	VBD	denotes	were
T2919	11462-11470	VBN	denotes	produced
T2924	11471-11473	IN	denotes	by
T2925	11474-11484	VBG	denotes	immunizing
T2926	11485-11492	NNS	denotes	rabbits
T2927	11492-11493	.	denotes	.
T2928	11493-11603	sentence	denotes	To test the specificity of the antibodies, we conducted Western-blot analysis of mouse brain tissue extracts.
T2929	11494-11496	TO	denotes	To
T2930	11497-11501	VB	denotes	test
T2932	11502-11505	DT	denotes	the
T2933	11506-11517	NN	denotes	specificity
T2934	11518-11520	IN	denotes	of
T2935	11521-11524	DT	denotes	the
T2936	11525-11535	NNS	denotes	antibodies
T2937	11535-11537	,	denotes	,
T2938	11537-11539	PRP	denotes	we
T2931	11540-11549	VBD	denotes	conducted
T2939	11550-11557	NNP	denotes	Western
T2941	11557-11558	HYPH	denotes	-
T2940	11558-11562	NN	denotes	blot
T2942	11563-11571	NN	denotes	analysis
T2943	11572-11574	IN	denotes	of
T2944	11575-11580	NN	denotes	mouse
T2945	11581-11586	NN	denotes	brain
T2946	11587-11593	NN	denotes	tissue
T2947	11594-11602	NNS	denotes	extracts
T2948	11602-11603	.	denotes	.
T2949	11603-11706	sentence	denotes	As shown in Fig. 6A, the antibody produced by C-terminal peptide specifically detected Acdp1 (lane 3).
T2950	11604-11606	IN	denotes	As
T2951	11607-11612	VBN	denotes	shown
T2953	11613-11615	IN	denotes	in
T2954	11616-11620	NN	denotes	Fig.
T2955	11621-11623	CD	denotes	6A
T2956	11623-11625	,	denotes	,
T2957	11625-11628	DT	denotes	the
T2958	11629-11637	NN	denotes	antibody
T2959	11638-11646	VBN	denotes	produced
T2960	11647-11649	IN	denotes	by
T2961	11650-11651	NN	denotes	C
T2963	11651-11652	HYPH	denotes	-
T2964	11652-11660	JJ	denotes	terminal
T2962	11661-11668	NN	denotes	peptide
T2965	11669-11681	RB	denotes	specifically
T2952	11682-11690	VBD	denotes	detected
T2966	11691-11696	NN	denotes	Acdp1
T2967	11697-11698	-LRB-	denotes	(
T2968	11698-11702	NN	denotes	lane
T2969	11703-11704	CD	denotes	3
T2970	11704-11705	-RRB-	denotes	)
T2971	11705-11706	.	denotes	.
T2972	11706-11868	sentence	denotes	The antibody generated by N-terminal peptide recognized Acdp4 in addition to Acdp1, although the reactivity to latter was significantly higher (Fig. 6A, lane 2).
T2973	11707-11710	DT	denotes	The
T2974	11711-11719	NN	denotes	antibody
T2976	11720-11729	VBN	denotes	generated
T2977	11730-11732	IN	denotes	by
T2978	11733-11734	NN	denotes	N
T2980	11734-11735	HYPH	denotes	-
T2981	11735-11743	JJ	denotes	terminal
T2979	11744-11751	NN	denotes	peptide
T2975	11752-11762	VBD	denotes	recognized
T2982	11763-11768	NN	denotes	Acdp4
T2983	11769-11771	IN	denotes	in
T2984	11772-11780	NN	denotes	addition
T2985	11781-11783	IN	denotes	to
T2986	11784-11789	NN	denotes	Acdp1
T2987	11789-11791	,	denotes	,
T2988	11791-11799	IN	denotes	although
T2990	11800-11803	DT	denotes	the
T2991	11804-11814	NN	denotes	reactivity
T2992	11815-11817	IN	denotes	to
T2993	11818-11824	NN	denotes	latter
T2989	11825-11828	VBD	denotes	was
T2994	11829-11842	RB	denotes	significantly
T2995	11843-11849	JJR	denotes	higher
T2996	11850-11851	-LRB-	denotes	(
T2998	11851-11855	NN	denotes	Fig.
T2999	11856-11858	CD	denotes	6A
T3000	11858-11860	,	denotes	,
T2997	11860-11864	NN	denotes	lane
T3001	11865-11866	CD	denotes	2
T3002	11866-11867	-RRB-	denotes	)
T3003	11867-11868	.	denotes	.
T3004	11868-11983	sentence	denotes	As expected, the antibody produced by the conserved sequence peptide detected all Acdp proteins (Fig. 6A, lane 1).
T3005	11869-11871	IN	denotes	As
T3006	11872-11880	VBN	denotes	expected
T3008	11880-11882	,	denotes	,
T3009	11882-11885	DT	denotes	the
T3010	11886-11894	NN	denotes	antibody
T3011	11895-11903	VBN	denotes	produced
T3012	11904-11906	IN	denotes	by
T3013	11907-11910	DT	denotes	the
T3015	11911-11920	VBN	denotes	conserved
T3016	11921-11929	NN	denotes	sequence
T3014	11930-11937	NN	denotes	peptide
T3007	11938-11946	VBD	denotes	detected
T3017	11947-11950	DT	denotes	all
T3019	11951-11955	NN	denotes	Acdp
T3018	11956-11964	NN	denotes	proteins
T3020	11965-11966	-LRB-	denotes	(
T3022	11966-11970	NN	denotes	Fig.
T3023	11971-11973	CD	denotes	6A
T3024	11973-11975	,	denotes	,
T3021	11975-11979	NN	denotes	lane
T3025	11980-11981	CD	denotes	1
T3026	11981-11982	-RRB-	denotes	)
T3027	11982-11983	.	denotes	.
T3028	11983-12118	sentence	denotes	To further determine the specificity of the antibody against the Acdp1 C-terminus, we analyzed extracts of HEK293, 3T3 and PC12 cells.
T3029	11984-11986	TO	denotes	To
T3031	11987-11994	RB	denotes	further
T3030	11995-12004	VB	denotes	determine
T3033	12005-12008	DT	denotes	the
T3034	12009-12020	NN	denotes	specificity
T3035	12021-12023	IN	denotes	of
T3036	12024-12027	DT	denotes	the
T3037	12028-12036	NN	denotes	antibody
T3038	12037-12044	IN	denotes	against
T3039	12045-12048	DT	denotes	the
T3041	12049-12054	NN	denotes	Acdp1
T3042	12055-12056	NN	denotes	C
T3043	12056-12057	HYPH	denotes	-
T3040	12057-12065	NN	denotes	terminus
T3044	12065-12067	,	denotes	,
T3045	12067-12069	PRP	denotes	we
T3032	12070-12078	VBD	denotes	analyzed
T3046	12079-12087	NNS	denotes	extracts
T3047	12088-12090	IN	denotes	of
T3048	12091-12097	NN	denotes	HEK293
T3050	12097-12099	,	denotes	,
T3051	12099-12102	NN	denotes	3T3
T3052	12103-12106	CC	denotes	and
T3053	12107-12111	NN	denotes	PC12
T3049	12112-12117	NNS	denotes	cells
T3054	12117-12118	.	denotes	.
T3055	12118-12152	sentence	denotes	The results are shown in Fig. 6B.
T3056	12119-12122	DT	denotes	The
T3057	12123-12130	NNS	denotes	results
T3059	12131-12134	VBP	denotes	are
T3058	12135-12140	VBN	denotes	shown
T3060	12141-12143	IN	denotes	in
T3061	12144-12148	NN	denotes	Fig.
T3062	12149-12151	CD	denotes	6B
T3063	12151-12152	.	denotes	.
T3064	12152-12233	sentence	denotes	Apparently, this antibody detected a specific band of Acdp1 in all cell lysates.
T3065	12153-12163	RB	denotes	Apparently
T3067	12163-12165	,	denotes	,
T3068	12165-12169	DT	denotes	this
T3069	12170-12178	NN	denotes	antibody
T3066	12179-12187	VBD	denotes	detected
T3070	12188-12189	DT	denotes	a
T3072	12190-12198	JJ	denotes	specific
T3071	12199-12203	NN	denotes	band
T3073	12204-12206	IN	denotes	of
T3074	12207-12212	NN	denotes	Acdp1
T3075	12213-12215	IN	denotes	in
T3076	12216-12219	DT	denotes	all
T3078	12220-12224	NN	denotes	cell
T3077	12225-12232	NNS	denotes	lysates
T3079	12232-12233	.	denotes	.
T3080	12233-12359	sentence	denotes	Of note, shown in Fig. 6B are signals of 10 μg extracts of HEK293 cell lysates, 100 μg extracts of 3T3 and PC12 cell lysates.
T3081	12234-12236	IN	denotes	Of
T3083	12237-12241	NN	denotes	note
T3084	12241-12243	,	denotes	,
T3085	12243-12248	VBN	denotes	shown
T3086	12249-12251	IN	denotes	in
T3087	12252-12256	NN	denotes	Fig.
T3088	12257-12259	CD	denotes	6B
T3082	12260-12263	VBP	denotes	are
T3089	12264-12271	NNS	denotes	signals
T3090	12272-12274	IN	denotes	of
T3091	12275-12277	CD	denotes	10
T3092	12278-12280	NN	denotes	μg
T3093	12281-12289	NNS	denotes	extracts
T3094	12290-12292	IN	denotes	of
T3095	12293-12299	NN	denotes	HEK293
T3097	12300-12304	NN	denotes	cell
T3096	12305-12312	NNS	denotes	lysates
T3098	12312-12314	,	denotes	,
T3099	12314-12317	CD	denotes	100
T3100	12318-12320	NN	denotes	μg
T3101	12321-12329	NNS	denotes	extracts
T3102	12330-12332	IN	denotes	of
T3103	12333-12336	NN	denotes	3T3
T3105	12337-12340	CC	denotes	and
T3106	12341-12345	NN	denotes	PC12
T3107	12346-12350	NN	denotes	cell
T3104	12351-12358	NNS	denotes	lysates
T3108	12358-12359	.	denotes	.
T3109	12359-12473	sentence	denotes	Thus, the expression levels of Acdp1 in these cell types vary a lot, with the highest expression in HEK293 cells.
T3110	12360-12364	RB	denotes	Thus
T3112	12364-12366	,	denotes	,
T3113	12366-12369	DT	denotes	the
T3115	12370-12380	NN	denotes	expression
T3114	12381-12387	NNS	denotes	levels
T3116	12388-12390	IN	denotes	of
T3117	12391-12396	NN	denotes	Acdp1
T3118	12397-12399	IN	denotes	in
T3119	12400-12405	DT	denotes	these
T3121	12406-12410	NN	denotes	cell
T3120	12411-12416	NNS	denotes	types
T3111	12417-12421	VBP	denotes	vary
T3122	12422-12423	DT	denotes	a
T3123	12424-12427	NN	denotes	lot
T3124	12427-12429	,	denotes	,
T3125	12429-12433	IN	denotes	with
T3126	12434-12437	DT	denotes	the
T3128	12438-12445	JJS	denotes	highest
T3127	12446-12456	NN	denotes	expression
T3129	12457-12459	IN	denotes	in
T3130	12460-12466	NN	denotes	HEK293
T3131	12467-12472	NNS	denotes	cells
T3132	12472-12473	.	denotes	.
T3133	12473-12633	sentence	denotes	Nevertheless, these immunoblot results support our analysis of brain tissue extracts that the antibody against Acdp1 C-terminus specifically recognizes Acdp-1.
T3134	12474-12486	RB	denotes	Nevertheless
T3136	12486-12488	,	denotes	,
T3137	12488-12493	DT	denotes	these
T3139	12494-12504	NN	denotes	immunoblot
T3138	12505-12512	NNS	denotes	results
T3135	12513-12520	VBP	denotes	support
T3140	12521-12524	PRP$	denotes	our
T3141	12525-12533	NN	denotes	analysis
T3142	12534-12536	IN	denotes	of
T3143	12537-12542	NN	denotes	brain
T3145	12543-12549	NN	denotes	tissue
T3144	12550-12558	NNS	denotes	extracts
T3146	12559-12563	IN	denotes	that
T3148	12564-12567	DT	denotes	the
T3149	12568-12576	NN	denotes	antibody
T3150	12577-12584	IN	denotes	against
T3151	12585-12590	NN	denotes	Acdp1
T3153	12591-12592	NN	denotes	C
T3154	12592-12593	HYPH	denotes	-
T3152	12593-12601	NN	denotes	terminus
T3155	12602-12614	RB	denotes	specifically
T3147	12615-12625	VBZ	denotes	recognizes
T3156	12626-12630	NN	denotes	Acdp
T3157	12630-12631	HYPH	denotes	-
T3158	12631-12632	CD	denotes	1
T3159	12632-12633	.	denotes	.
T3160	12633-12752	sentence	denotes	The specificity of the Acdp1 C-terminus antibody suggests the possibility of using it to localize Acdp-1 within cells.
T3161	12634-12637	DT	denotes	The
T3162	12638-12649	NN	denotes	specificity
T3164	12650-12652	IN	denotes	of
T3165	12653-12656	DT	denotes	the
T3167	12657-12662	NN	denotes	Acdp1
T3168	12663-12664	NN	denotes	C
T3170	12664-12665	HYPH	denotes	-
T3169	12665-12673	NN	denotes	terminus
T3166	12674-12682	NN	denotes	antibody
T3163	12683-12691	VBZ	denotes	suggests
T3171	12692-12695	DT	denotes	the
T3172	12696-12707	NN	denotes	possibility
T3173	12708-12710	IN	denotes	of
T3174	12711-12716	VBG	denotes	using
T3175	12717-12719	PRP	denotes	it
T3176	12720-12722	TO	denotes	to
T3177	12723-12731	VB	denotes	localize
T3178	12732-12736	NN	denotes	Acdp
T3179	12736-12737	HYPH	denotes	-
T3180	12737-12738	CD	denotes	1
T3181	12739-12745	IN	denotes	within
T3182	12746-12751	NNS	denotes	cells
T3183	12751-12752	.	denotes	.
T3184	12752-12925	sentence	denotes	Since Northern blot revealed almost exclusive expression of Acdp1 in the brain, we examined its subcellular localization in hippocampus neurons isolated from mouse embryos.
T3185	12753-12758	IN	denotes	Since
T3187	12759-12767	NNP	denotes	Northern
T3188	12768-12772	NN	denotes	blot
T3186	12773-12781	VBD	denotes	revealed
T3190	12782-12788	RB	denotes	almost
T3191	12789-12798	JJ	denotes	exclusive
T3192	12799-12809	NN	denotes	expression
T3193	12810-12812	IN	denotes	of
T3194	12813-12818	NN	denotes	Acdp1
T3195	12819-12821	IN	denotes	in
T3196	12822-12825	DT	denotes	the
T3197	12826-12831	NN	denotes	brain
T3198	12831-12833	,	denotes	,
T3199	12833-12835	PRP	denotes	we
T3189	12836-12844	VBD	denotes	examined
T3200	12845-12848	PRP$	denotes	its
T3202	12849-12860	JJ	denotes	subcellular
T3201	12861-12873	NN	denotes	localization
T3203	12874-12876	IN	denotes	in
T3204	12877-12888	NN	denotes	hippocampus
T3205	12889-12896	NNS	denotes	neurons
T3206	12897-12905	VBN	denotes	isolated
T3207	12906-12910	IN	denotes	from
T3208	12911-12916	NN	denotes	mouse
T3209	12917-12924	NNS	denotes	embryos
T3210	12924-12925	.	denotes	.
T3211	12925-13045	sentence	denotes	The neurons were cultured on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes in dishes.
T3212	12926-12929	DT	denotes	The
T3213	12930-12937	NNS	denotes	neurons
T3215	12938-12942	VBD	denotes	were
T3214	12943-12951	VBN	denotes	cultured
T3216	12952-12954	IN	denotes	on
T3217	12955-12960	NN	denotes	glass
T3218	12961-12971	NNS	denotes	coverslips
T3219	12972-12978	VBN	denotes	coated
T3220	12979-12983	IN	denotes	with
T3221	12984-12985	DT	denotes	a
T3223	12986-12995	JJ	denotes	confluent
T3222	12996-13005	NN	denotes	monolayer
T3224	13006-13008	IN	denotes	of
T3225	13009-13014	NN	denotes	mouse
T3227	13015-13023	JJ	denotes	cortical
T3226	13024-13034	NNS	denotes	astrocytes
T3228	13035-13037	IN	denotes	in
T3229	13038-13044	NNS	denotes	dishes
T3230	13044-13045	.	denotes	.
T3231	13045-13110	sentence	denotes	Immunostaining was using the Acdp1 C-terminus specific antibody.
T3232	13046-13060	NN	denotes	Immunostaining
T3234	13061-13064	VBD	denotes	was
T3233	13065-13070	VBG	denotes	using
T3235	13071-13074	DT	denotes	the
T3237	13075-13080	NN	denotes	Acdp1
T3238	13081-13082	NN	denotes	C
T3240	13082-13083	HYPH	denotes	-
T3239	13083-13091	NN	denotes	terminus
T3241	13092-13100	JJ	denotes	specific
T3236	13101-13109	NN	denotes	antibody
T3242	13109-13110	.	denotes	.
T3243	13110-13198	sentence	denotes	Confocal imaging revealed that Acdp1 is predominantly localized on the plasma membrane.
T3244	13111-13119	JJ	denotes	Confocal
T3245	13120-13127	NN	denotes	imaging
T3246	13128-13136	VBD	denotes	revealed
T3247	13137-13141	IN	denotes	that
T3249	13142-13147	NN	denotes	Acdp1
T3250	13148-13150	VBZ	denotes	is
T3251	13151-13164	RB	denotes	predominantly
T3248	13165-13174	VBN	denotes	localized
T3252	13175-13177	IN	denotes	on
T3253	13178-13181	DT	denotes	the
T3255	13182-13188	NN	denotes	plasma
T3254	13189-13197	NN	denotes	membrane
T3256	13197-13198	.	denotes	.
T3257	13198-13425	sentence	denotes	A series of sections of a cell at the thickness of 0.5 micrometer clearly showed membrane location of Acdp1-immunoreactivity (Fig. 7), which is consistent with the observation of transmembrane domains within the Acdp proteins.
T3258	13199-13200	DT	denotes	A
T3259	13201-13207	NN	denotes	series
T3261	13208-13210	IN	denotes	of
T3262	13211-13219	NNS	denotes	sections
T3263	13220-13222	IN	denotes	of
T3264	13223-13224	DT	denotes	a
T3265	13225-13229	NN	denotes	cell
T3266	13230-13232	IN	denotes	at
T3267	13233-13236	DT	denotes	the
T3268	13237-13246	NN	denotes	thickness
T3269	13247-13249	IN	denotes	of
T3270	13250-13253	CD	denotes	0.5
T3271	13254-13264	NN	denotes	micrometer
T3272	13265-13272	RB	denotes	clearly
T3260	13273-13279	VBD	denotes	showed
T3273	13280-13288	NN	denotes	membrane
T3274	13289-13297	NN	denotes	location
T3275	13298-13300	IN	denotes	of
T3276	13301-13306	NN	denotes	Acdp1
T3278	13306-13307	HYPH	denotes	-
T3277	13307-13323	NN	denotes	immunoreactivity
T3279	13324-13325	-LRB-	denotes	(
T3280	13325-13329	NN	denotes	Fig.
T3281	13330-13331	CD	denotes	7
T3282	13331-13332	-RRB-	denotes	)
T3283	13332-13334	,	denotes	,
T3284	13334-13339	WDT	denotes	which
T3285	13340-13342	VBZ	denotes	is
T3286	13343-13353	JJ	denotes	consistent
T3287	13354-13358	IN	denotes	with
T3288	13359-13362	DT	denotes	the
T3289	13363-13374	NN	denotes	observation
T3290	13375-13377	IN	denotes	of
T3291	13378-13391	NN	denotes	transmembrane
T3292	13392-13399	NNS	denotes	domains
T3293	13400-13406	IN	denotes	within
T3294	13407-13410	DT	denotes	the
T3296	13411-13415	NN	denotes	Acdp
T3295	13416-13424	NN	denotes	proteins
T3297	13424-13425	.	denotes	.
T6368	13436-13440	NN	denotes	Fig.
T6370	13441-13443	CD	denotes	6A
T6371	13443-13445	:	denotes	:
T6372	13445-13455	NN	denotes	Immunoblot
T6369	13456-13464	NN	denotes	analysis
T6373	13465-13467	IN	denotes	of
T6374	13468-13472	NN	denotes	Acdp
T6375	13473-13481	NN	denotes	proteins
T6376	13482-13484	IN	denotes	in
T6377	13485-13490	NN	denotes	brain
T6378	13491-13497	NN	denotes	tissue
T6379	13498-13506	NNS	denotes	extracts
T6380	13506-13507	.	denotes	.
T6381	13507-13597	sentence	denotes	Immunoblotting were carried out using a Western blotting detection system (ECL) (PIERCE).
T6382	13508-13522	NN	denotes	Immunoblotting
T6384	13523-13527	VBD	denotes	were
T6383	13528-13535	VBN	denotes	carried
T6385	13536-13539	RP	denotes	out
T6386	13540-13545	VBG	denotes	using
T6387	13546-13547	DT	denotes	a
T6389	13548-13555	NNP	denotes	Western
T6390	13556-13564	NN	denotes	blotting
T6391	13565-13574	NN	denotes	detection
T6388	13575-13581	NN	denotes	system
T6392	13582-13583	-LRB-	denotes	(
T6393	13583-13586	NN	denotes	ECL
T6394	13586-13587	-RRB-	denotes	)
T6395	13588-13589	-LRB-	denotes	(
T6396	13589-13595	NN	denotes	PIERCE
T6397	13595-13596	-RRB-	denotes	)
T6398	13596-13597	.	denotes	.
T6399	13597-13658	sentence	denotes	Lane 1, probed with antibody generated by conserved peptide.
T6400	13598-13602	NN	denotes	Lane
T6402	13603-13604	CD	denotes	1
T6403	13604-13606	,	denotes	,
T6401	13606-13612	VBN	denotes	probed
T6404	13613-13617	IN	denotes	with
T6405	13618-13626	NN	denotes	antibody
T6406	13627-13636	VBN	denotes	generated
T6407	13637-13639	IN	denotes	by
T6408	13640-13649	JJ	denotes	conserved
T6409	13650-13657	NN	denotes	peptide
T6410	13657-13658	.	denotes	.
T6411	13658-13770	sentence	denotes	From top to bottom, each band corresponding to Acdp1 (115 kD), Acdp2 (100 kD), Acdp4 (90 kD) and Acdp3 (80 kD).
T6412	13659-13663	IN	denotes	From
T6414	13664-13667	NN	denotes	top
T6415	13668-13670	IN	denotes	to
T6416	13671-13677	NN	denotes	bottom
T6417	13677-13679	,	denotes	,
T6418	13679-13683	DT	denotes	each
T6413	13684-13688	NN	denotes	band
T6419	13689-13702	VBG	denotes	corresponding
T6420	13703-13705	IN	denotes	to
T6421	13706-13711	NN	denotes	Acdp1
T6422	13712-13713	-LRB-	denotes	(
T6424	13713-13716	CD	denotes	115
T6423	13717-13719	NN	denotes	kD
T6425	13719-13720	-RRB-	denotes	)
T6426	13720-13722	,	denotes	,
T6427	13722-13727	NN	denotes	Acdp2
T6428	13728-13729	-LRB-	denotes	(
T6430	13729-13732	CD	denotes	100
T6429	13733-13735	NN	denotes	kD
T6431	13735-13736	-RRB-	denotes	)
T6432	13736-13738	,	denotes	,
T6433	13738-13743	NN	denotes	Acdp4
T6434	13744-13745	-LRB-	denotes	(
T6436	13745-13747	CD	denotes	90
T6435	13748-13750	NN	denotes	kD
T6437	13750-13751	-RRB-	denotes	)
T6438	13752-13755	CC	denotes	and
T6439	13756-13761	NN	denotes	Acdp3
T6440	13762-13763	-LRB-	denotes	(
T6442	13763-13765	CD	denotes	80
T6441	13766-13768	NN	denotes	kD
T6443	13768-13769	-RRB-	denotes	)
T6444	13769-13770	.	denotes	.
T6445	13770-13880	sentence	denotes	Lane 2 and 3, probed with the Acdp1 antibodies generated by N-terminal and C-terminal peptides, respectively.
T6446	13771-13775	NN	denotes	Lane
T6447	13776-13777	CD	denotes	2
T6449	13778-13781	CC	denotes	and
T6450	13782-13783	CD	denotes	3
T6451	13783-13785	,	denotes	,
T6448	13785-13791	VBN	denotes	probed
T6452	13792-13796	IN	denotes	with
T6453	13797-13800	DT	denotes	the
T6455	13801-13806	NN	denotes	Acdp1
T6454	13807-13817	NNS	denotes	antibodies
T6456	13818-13827	VBN	denotes	generated
T6457	13828-13830	IN	denotes	by
T6458	13831-13832	NN	denotes	N
T6460	13832-13833	HYPH	denotes	-
T6459	13833-13841	JJ	denotes	terminal
T6462	13842-13845	CC	denotes	and
T6463	13846-13847	NN	denotes	C
T6465	13847-13848	HYPH	denotes	-
T6464	13848-13856	JJ	denotes	terminal
T6461	13857-13865	NNS	denotes	peptides
T6466	13865-13867	,	denotes	,
T6467	13867-13879	RB	denotes	respectively
T6468	13879-13880	.	denotes	.
T6469	13880-13949	sentence	denotes	Fig. 6B: Immunoblot analysis of Acdp1 in HEK293, 3T3 and PC12 cells.
T6470	13881-13885	NN	denotes	Fig.
T6472	13886-13888	CD	denotes	6B
T6473	13888-13890	:	denotes	:
T6474	13890-13900	NN	denotes	Immunoblot
T6471	13901-13909	NN	denotes	analysis
T6475	13910-13912	IN	denotes	of
T6476	13913-13918	NN	denotes	Acdp1
T6477	13919-13921	IN	denotes	in
T6478	13922-13928	NN	denotes	HEK293
T6480	13928-13930	,	denotes	,
T6481	13930-13933	NN	denotes	3T3
T6482	13934-13937	CC	denotes	and
T6483	13938-13942	NN	denotes	PC12
T6479	13943-13948	NNS	denotes	cells
T6484	13948-13949	.	denotes	.
T6485	13949-14023	sentence	denotes	The blots were probed with the antibody against the C-terminus of Acdp-1.
T6486	13950-13953	DT	denotes	The
T6487	13954-13959	NNS	denotes	blots
T6489	13960-13964	VBD	denotes	were
T6488	13965-13971	VBN	denotes	probed
T6490	13972-13976	IN	denotes	with
T6491	13977-13980	DT	denotes	the
T6492	13981-13989	NN	denotes	antibody
T6493	13990-13997	IN	denotes	against
T6494	13998-14001	DT	denotes	the
T6496	14002-14003	NN	denotes	C
T6497	14003-14004	HYPH	denotes	-
T6495	14004-14012	NN	denotes	terminus
T6498	14013-14015	IN	denotes	of
T6499	14016-14020	NN	denotes	Acdp
T6500	14020-14021	HYPH	denotes	-
T6501	14021-14022	CD	denotes	1
T6502	14022-14023	.	denotes	.
T6503	14023-14061	sentence	denotes	Lane 1, 10 μg of HEK293 cell lysates.
T6504	14024-14028	NN	denotes	Lane
T6506	14029-14030	CD	denotes	1
T6507	14030-14032	,	denotes	,
T6508	14032-14034	CD	denotes	10
T6505	14035-14037	NNS	denotes	μg
T6509	14038-14040	IN	denotes	of
T6510	14041-14047	NN	denotes	HEK293
T6512	14048-14052	NN	denotes	cell
T6511	14053-14060	NNS	denotes	lysates
T6513	14060-14061	.	denotes	.
T6514	14061-14112	sentence	denotes	Lane 2 and 3, 100 μg of 3T3 and PC12 cell lysates.
T6515	14062-14066	NN	denotes	Lane
T6516	14067-14068	CD	denotes	2
T6518	14069-14072	CC	denotes	and
T6519	14073-14074	CD	denotes	3
T6520	14074-14076	,	denotes	,
T6521	14076-14079	CD	denotes	100
T6517	14080-14082	NNS	denotes	μg
T6522	14083-14085	IN	denotes	of
T6523	14086-14089	NN	denotes	3T3
T6525	14090-14093	CC	denotes	and
T6526	14094-14098	NN	denotes	PC12
T6527	14099-14103	NN	denotes	cell
T6524	14104-14111	NNS	denotes	lysates
T6528	14111-14112	.	denotes	.
T6553	14123-14134	JJ	denotes	Subcellular
T6554	14135-14147	NN	denotes	localization
T6555	14148-14150	IN	denotes	of
T6556	14151-14156	NN	denotes	Acdp1
T6557	14157-14159	IN	denotes	in
T6558	14160-14171	NN	denotes	hippocampus
T6559	14172-14179	NNS	denotes	neurons
T6560	14179-14180	.	denotes	.
T6561	14180-14268	sentence	denotes	A series of confocal images from a cultured neuron stained with an anti-Acdp1 antibody.
T6562	14181-14182	DT	denotes	A
T6563	14183-14189	NN	denotes	series
T6564	14190-14192	IN	denotes	of
T6565	14193-14201	JJ	denotes	confocal
T6566	14202-14208	NNS	denotes	images
T6567	14209-14213	IN	denotes	from
T6568	14214-14215	DT	denotes	a
T6570	14216-14224	VBN	denotes	cultured
T6569	14225-14231	NN	denotes	neuron
T6571	14232-14239	VBN	denotes	stained
T6572	14240-14244	IN	denotes	with
T6573	14245-14247	DT	denotes	an
T6575	14248-14258	JJ	denotes	anti-Acdp1
T6574	14259-14267	NN	denotes	antibody
T6576	14267-14268	.	denotes	.
T6577	14268-14379	sentence	denotes	The step of each imaging section is 0.5 μm, from the surface of the neuron (0 μm) to the middle plan (4.5 μm).
T6578	14269-14272	DT	denotes	The
T6579	14273-14277	NN	denotes	step
T6581	14278-14280	IN	denotes	of
T6582	14281-14285	DT	denotes	each
T6584	14286-14293	NN	denotes	imaging
T6583	14294-14301	NN	denotes	section
T6580	14302-14304	VBZ	denotes	is
T6585	14305-14308	CD	denotes	0.5
T6586	14309-14311	NNS	denotes	μm
T6587	14311-14313	,	denotes	,
T6588	14313-14317	IN	denotes	from
T6589	14318-14321	DT	denotes	the
T6590	14322-14329	NN	denotes	surface
T6591	14330-14332	IN	denotes	of
T6592	14333-14336	DT	denotes	the
T6593	14337-14343	NN	denotes	neuron
T6594	14344-14345	-LRB-	denotes	(
T6596	14345-14346	CD	denotes	0
T6595	14347-14349	NNS	denotes	μm
T6597	14349-14350	-RRB-	denotes	)
T6598	14351-14353	IN	denotes	to
T6599	14354-14357	DT	denotes	the
T6601	14358-14364	JJ	denotes	middle
T6600	14365-14369	NN	denotes	plan
T6602	14370-14371	-LRB-	denotes	(
T6604	14371-14374	CD	denotes	4.5
T6603	14375-14377	NNS	denotes	μm
T6605	14377-14378	-RRB-	denotes	)
T6606	14378-14379	.	denotes	.
T3529	14392-14400	IN	denotes	Although
T3531	14401-14404	DT	denotes	the
T3533	14405-14410	JJ	denotes	exact
T3532	14411-14420	NNS	denotes	functions
T3534	14421-14423	IN	denotes	of
T3535	14424-14427	DT	denotes	the
T3537	14428-14432	NN	denotes	ACDP
T3538	14433-14437	NN	denotes	gene
T3536	14438-14444	NN	denotes	family
T3539	14445-14448	VBP	denotes	are
T3540	14449-14452	RB	denotes	not
T3541	14453-14456	RB	denotes	yet
T3530	14457-14467	VBN	denotes	elucidated
T3543	14467-14469	,	denotes	,
T3544	14469-14476	JJ	denotes	several
T3545	14477-14482	NNS	denotes	lines
T3546	14483-14485	IN	denotes	of
T3547	14486-14494	NN	denotes	evidence
T3542	14495-14502	VBP	denotes	suggest
T3548	14503-14507	IN	denotes	that
T3550	14508-14512	NN	denotes	ACDP
T3551	14513-14518	NNS	denotes	genes
T3552	14519-14522	MD	denotes	may
T3549	14523-14527	VB	denotes	play
T3553	14528-14530	DT	denotes	an
T3555	14531-14540	JJ	denotes	important
T3554	14541-14545	NN	denotes	role
T3556	14546-14548	IN	denotes	in
T3557	14549-14559	JJ	denotes	biological
T3558	14560-14569	NNS	denotes	processes
T3559	14569-14570	.	denotes	.
T3560	14570-14818	sentence	denotes	First, these genes are evolutionarily conserved in many phylogenetically divergent species; Second, multiple genes are present in a species; Third, these genes are ubiquitously expressed throughout development and adult tissues (unpublished data).
T3561	14571-14576	RB	denotes	First
T3563	14576-14578	,	denotes	,
T3564	14578-14583	DT	denotes	these
T3565	14584-14589	NNS	denotes	genes
T3566	14590-14593	VBP	denotes	are
T3567	14594-14608	RB	denotes	evolutionarily
T3562	14609-14618	VBN	denotes	conserved
T3569	14619-14621	IN	denotes	in
T3570	14622-14626	JJ	denotes	many
T3572	14627-14643	RB	denotes	phylogenetically
T3573	14644-14653	JJ	denotes	divergent
T3571	14654-14661	NNS	denotes	species
T3574	14661-14662	:	denotes	;
T3575	14663-14669	RB	denotes	Second
T3577	14669-14671	,	denotes	,
T3578	14671-14679	JJ	denotes	multiple
T3579	14680-14685	NNS	denotes	genes
T3576	14686-14689	VBP	denotes	are
T3580	14690-14697	JJ	denotes	present
T3581	14698-14700	IN	denotes	in
T3582	14701-14702	DT	denotes	a
T3583	14703-14710	NN	denotes	species
T3584	14710-14711	:	denotes	;
T3585	14712-14717	RB	denotes	Third
T3586	14717-14719	,	denotes	,
T3587	14719-14724	DT	denotes	these
T3588	14725-14730	NNS	denotes	genes
T3589	14731-14734	VBP	denotes	are
T3590	14735-14747	RB	denotes	ubiquitously
T3568	14748-14757	VBN	denotes	expressed
T3591	14758-14768	IN	denotes	throughout
T3592	14769-14780	NN	denotes	development
T3594	14781-14784	CC	denotes	and
T3595	14785-14790	JJ	denotes	adult
T3593	14791-14798	NNS	denotes	tissues
T3596	14799-14800	-LRB-	denotes	(
T3598	14800-14811	JJ	denotes	unpublished
T3597	14812-14816	NNS	denotes	data
T3599	14816-14817	-RRB-	denotes	)
T3600	14817-14818	.	denotes	.
T3601	14818-14974	sentence	denotes	The cloning and characterization of the mouse Acdp gene family are a very important step towards the elucidation of the functions of this multigene family.
T3602	14819-14822	DT	denotes	The
T3603	14823-14830	NN	denotes	cloning
T3605	14831-14834	CC	denotes	and
T3606	14835-14851	NN	denotes	characterization
T3607	14852-14854	IN	denotes	of
T3608	14855-14858	DT	denotes	the
T3610	14859-14864	NN	denotes	mouse
T3611	14865-14869	NN	denotes	Acdp
T3612	14870-14874	NN	denotes	gene
T3609	14875-14881	NN	denotes	family
T3604	14882-14885	VBP	denotes	are
T3613	14886-14887	DT	denotes	a
T3615	14888-14892	RB	denotes	very
T3616	14893-14902	JJ	denotes	important
T3614	14903-14907	NN	denotes	step
T3617	14908-14915	IN	denotes	towards
T3618	14916-14919	DT	denotes	the
T3619	14920-14931	NN	denotes	elucidation
T3620	14932-14934	IN	denotes	of
T3621	14935-14938	DT	denotes	the
T3622	14939-14948	NNS	denotes	functions
T3623	14949-14951	IN	denotes	of
T3624	14952-14956	DT	denotes	this
T3626	14957-14966	JJ	denotes	multigene
T3625	14967-14973	NN	denotes	family
T3627	14973-14974	.	denotes	.
T3628	14974-15116	sentence	denotes	Sequence homology analyses revealed that Acdp proteins shared very strong AA homologies to the bacteria CorC protein and yeast Amip3 protein.
T3629	14975-14983	NN	denotes	Sequence
T3631	14984-14992	NN	denotes	homology
T3630	14993-15001	NNS	denotes	analyses
T3632	15002-15010	VBD	denotes	revealed
T3633	15011-15015	IN	denotes	that
T3635	15016-15020	NN	denotes	Acdp
T3636	15021-15029	NN	denotes	proteins
T3634	15030-15036	VBD	denotes	shared
T3637	15037-15041	RB	denotes	very
T3638	15042-15048	JJ	denotes	strong
T3640	15049-15051	NN	denotes	AA
T3639	15052-15062	NNS	denotes	homologies
T3641	15063-15065	IN	denotes	to
T3642	15066-15069	DT	denotes	the
T3644	15070-15078	NNS	denotes	bacteria
T3645	15079-15083	NN	denotes	CorC
T3643	15084-15091	NN	denotes	protein
T3646	15092-15095	CC	denotes	and
T3647	15096-15101	NN	denotes	yeast
T3649	15102-15107	NN	denotes	Amip3
T3648	15108-15115	NN	denotes	protein
T3650	15115-15116	.	denotes	.
T3651	15116-15220	sentence	denotes	CorA, B, C and D belong to a protein family involved in both influx and efflux of magnesium and cobalt.
T3652	15117-15121	NN	denotes	CorA
T3654	15121-15123	,	denotes	,
T3655	15123-15124	NN	denotes	B
T3656	15124-15126	,	denotes	,
T3657	15126-15127	NN	denotes	C
T3658	15128-15131	CC	denotes	and
T3659	15132-15133	NN	denotes	D
T3653	15134-15140	VBP	denotes	belong
T3660	15141-15143	IN	denotes	to
T3661	15144-15145	DT	denotes	a
T3663	15146-15153	NN	denotes	protein
T3662	15154-15160	NN	denotes	family
T3664	15161-15169	VBN	denotes	involved
T3665	15170-15172	IN	denotes	in
T3666	15173-15177	CC	denotes	both
T3667	15178-15184	NN	denotes	influx
T3668	15185-15188	CC	denotes	and
T3669	15189-15195	NN	denotes	efflux
T3670	15196-15198	IN	denotes	of
T3671	15199-15208	NN	denotes	magnesium
T3672	15209-15212	CC	denotes	and
T3673	15213-15219	NN	denotes	cobalt
T3674	15219-15220	.	denotes	.
T3675	15220-15298	sentence	denotes	It has been shown that CorA mutants confer resistance to cobalt toxicity [9].
T3676	15221-15223	PRP	denotes	It
T3678	15224-15227	VBZ	denotes	has
T3679	15228-15232	VBN	denotes	been
T3677	15233-15238	VBN	denotes	shown
T3680	15239-15243	IN	denotes	that
T3682	15244-15248	NN	denotes	CorA
T3683	15249-15256	NNS	denotes	mutants
T3681	15257-15263	VBP	denotes	confer
T3684	15264-15274	NN	denotes	resistance
T3685	15275-15277	IN	denotes	to
T3686	15278-15284	NN	denotes	cobalt
T3687	15285-15293	NN	denotes	toxicity
T3688	15294-15295	-LRB-	denotes	[
T3689	15295-15296	CD	denotes	9
T3690	15296-15297	-RRB-	denotes	]
T3691	15297-15298	.	denotes	.
T3692	15298-15412	sentence	denotes	Amip3 appears to be a homologous to CorC protein which is involved in efflux of magnesium and cobalt in bacteria.
T3693	15299-15304	NN	denotes	Amip3
T3694	15305-15312	VBZ	denotes	appears
T3695	15313-15315	TO	denotes	to
T3696	15316-15318	VB	denotes	be
T3697	15319-15320	DT	denotes	a
T3698	15321-15331	JJ	denotes	homologous
T3699	15332-15334	IN	denotes	to
T3700	15335-15339	NN	denotes	CorC
T3701	15340-15347	NN	denotes	protein
T3702	15348-15353	WDT	denotes	which
T3704	15354-15356	VBZ	denotes	is
T3703	15357-15365	VBN	denotes	involved
T3705	15366-15368	IN	denotes	in
T3706	15369-15375	NN	denotes	efflux
T3707	15376-15378	IN	denotes	of
T3708	15379-15388	NN	denotes	magnesium
T3709	15389-15392	CC	denotes	and
T3710	15393-15399	NN	denotes	cobalt
T3711	15400-15402	IN	denotes	in
T3712	15403-15411	NNS	denotes	bacteria
T3713	15411-15412	.	denotes	.
T3714	15412-15463	sentence	denotes	Amip3 mutants confer resistant to copper toxicity.
T3715	15413-15418	NN	denotes	Amip3
T3716	15419-15426	NNS	denotes	mutants
T3717	15427-15433	VBP	denotes	confer
T3718	15434-15443	NN	denotes	resistant
T3719	15444-15446	IN	denotes	to
T3720	15447-15453	NN	denotes	copper
T3721	15454-15462	NN	denotes	toxicity
T3722	15462-15463	.	denotes	.
T3723	15463-15625	sentence	denotes	Acdp proteins also possess the domains that are found in bacteria CorC and yeast Amip3 proteins, such as the CBS domains, DUF21 domain and transmembrane domains.
T3724	15464-15468	NN	denotes	Acdp
T3726	15469-15477	NN	denotes	proteins
T3727	15478-15482	RB	denotes	also
T3725	15483-15490	VBP	denotes	possess
T3728	15491-15494	DT	denotes	the
T3729	15495-15502	NNS	denotes	domains
T3730	15503-15507	WDT	denotes	that
T3732	15508-15511	VBP	denotes	are
T3731	15512-15517	VBN	denotes	found
T3733	15518-15520	IN	denotes	in
T3734	15521-15529	NNS	denotes	bacteria
T3735	15530-15534	NN	denotes	CorC
T3737	15535-15538	CC	denotes	and
T3738	15539-15544	NN	denotes	yeast
T3739	15545-15550	NN	denotes	Amip3
T3736	15551-15559	NN	denotes	proteins
T3740	15559-15561	,	denotes	,
T3741	15561-15565	JJ	denotes	such
T3742	15566-15568	IN	denotes	as
T3743	15569-15572	DT	denotes	the
T3745	15573-15576	NN	denotes	CBS
T3744	15577-15584	NNS	denotes	domains
T3746	15584-15586	,	denotes	,
T3747	15586-15591	NN	denotes	DUF21
T3748	15592-15598	NN	denotes	domain
T3749	15599-15602	CC	denotes	and
T3750	15603-15616	NN	denotes	transmembrane
T3751	15617-15624	NNS	denotes	domains
T3752	15624-15625	.	denotes	.
T3753	15625-15769	sentence	denotes	In addition, a cNMP-binding domain was found in all Acdp proteins, which is usually present in ion channels and cNMP-dependent kinases [10-13].
T3754	15626-15628	IN	denotes	In
T3756	15629-15637	NN	denotes	addition
T3757	15637-15639	,	denotes	,
T3758	15639-15640	DT	denotes	a
T3760	15641-15645	NN	denotes	cNMP
T3762	15645-15646	HYPH	denotes	-
T3761	15646-15653	VBG	denotes	binding
T3759	15654-15660	NN	denotes	domain
T3763	15661-15664	VBD	denotes	was
T3755	15665-15670	VBN	denotes	found
T3764	15671-15673	IN	denotes	in
T3765	15674-15677	DT	denotes	all
T3767	15678-15682	NN	denotes	Acdp
T3766	15683-15691	NN	denotes	proteins
T3768	15691-15693	,	denotes	,
T3769	15693-15698	WDT	denotes	which
T3770	15699-15701	VBZ	denotes	is
T3771	15702-15709	RB	denotes	usually
T3772	15710-15717	JJ	denotes	present
T3773	15718-15720	IN	denotes	in
T3774	15721-15724	NN	denotes	ion
T3775	15725-15733	NNS	denotes	channels
T3776	15734-15737	CC	denotes	and
T3777	15738-15742	NN	denotes	cNMP
T3779	15742-15743	HYPH	denotes	-
T3780	15743-15752	JJ	denotes	dependent
T3778	15753-15760	NNS	denotes	kinases
T3781	15761-15762	-LRB-	denotes	[
T3782	15762-15764	CD	denotes	10
T3783	15764-15765	SYM	denotes	-
T3784	15765-15767	CD	denotes	13
T3785	15767-15768	-RRB-	denotes	]
T3786	15768-15769	.	denotes	.
T3787	15769-15921	sentence	denotes	Using antibody produced by Acdp1 C-terminal peptide, we have shown that Acdp1 is predominantly localized on the plasma membrane in hippocampus neurons.
T3788	15770-15775	VBG	denotes	Using
T3790	15776-15784	NN	denotes	antibody
T3791	15785-15793	VBN	denotes	produced
T3792	15794-15796	IN	denotes	by
T3793	15797-15802	NN	denotes	Acdp1
T3795	15803-15804	NN	denotes	C
T3797	15804-15805	HYPH	denotes	-
T3796	15805-15813	JJ	denotes	terminal
T3794	15814-15821	NN	denotes	peptide
T3798	15821-15823	,	denotes	,
T3799	15823-15825	PRP	denotes	we
T3800	15826-15830	VBP	denotes	have
T3789	15831-15836	VBN	denotes	shown
T3801	15837-15841	IN	denotes	that
T3803	15842-15847	NN	denotes	Acdp1
T3804	15848-15850	VBZ	denotes	is
T3805	15851-15864	RB	denotes	predominantly
T3802	15865-15874	VBN	denotes	localized
T3806	15875-15877	IN	denotes	on
T3807	15878-15881	DT	denotes	the
T3809	15882-15888	NN	denotes	plasma
T3808	15889-15897	NN	denotes	membrane
T3810	15898-15900	IN	denotes	in
T3811	15901-15912	NN	denotes	hippocampus
T3812	15913-15920	NNS	denotes	neurons
T3813	15920-15921	.	denotes	.
T3814	15921-16035	sentence	denotes	In our previous study, we found human ACDP proteins are predominantly localized in the nucleus in HeLa cells [1].
T3815	15922-15924	IN	denotes	In
T3817	15925-15928	PRP$	denotes	our
T3819	15929-15937	JJ	denotes	previous
T3818	15938-15943	NN	denotes	study
T3820	15943-15945	,	denotes	,
T3821	15945-15947	PRP	denotes	we
T3816	15948-15953	VBD	denotes	found
T3822	15954-15959	JJ	denotes	human
T3824	15960-15964	NN	denotes	ACDP
T3823	15965-15973	NN	denotes	proteins
T3826	15974-15977	VBP	denotes	are
T3827	15978-15991	RB	denotes	predominantly
T3825	15992-16001	VBN	denotes	localized
T3828	16002-16004	IN	denotes	in
T3829	16005-16008	DT	denotes	the
T3830	16009-16016	NN	denotes	nucleus
T3831	16017-16019	IN	denotes	in
T3832	16020-16024	NN	denotes	HeLa
T3833	16025-16030	NNS	denotes	cells
T3834	16031-16032	-LRB-	denotes	[
T3835	16032-16033	CD	denotes	1
T3836	16033-16034	-RRB-	denotes	]
T3837	16034-16035	.	denotes	.
T3838	16035-16223	sentence	denotes	The discrepancy for Acdp localization in neuronal cells could be caused by non-specificity for previous antibody which was produced by the recombinant ACD domain, or different cells used.
T3839	16036-16039	DT	denotes	The
T3840	16040-16051	NN	denotes	discrepancy
T3842	16052-16055	IN	denotes	for
T3843	16056-16060	NN	denotes	Acdp
T3844	16061-16073	NN	denotes	localization
T3845	16074-16076	IN	denotes	in
T3846	16077-16085	JJ	denotes	neuronal
T3847	16086-16091	NNS	denotes	cells
T3848	16092-16097	MD	denotes	could
T3849	16098-16100	VB	denotes	be
T3841	16101-16107	VBN	denotes	caused
T3850	16108-16110	IN	denotes	by
T3851	16111-16126	NN	denotes	non-specificity
T3852	16127-16130	IN	denotes	for
T3853	16131-16139	JJ	denotes	previous
T3854	16140-16148	NN	denotes	antibody
T3855	16149-16154	WDT	denotes	which
T3857	16155-16158	VBD	denotes	was
T3856	16159-16167	VBN	denotes	produced
T3858	16168-16170	IN	denotes	by
T3859	16171-16174	DT	denotes	the
T3861	16175-16186	JJ	denotes	recombinant
T3862	16187-16190	NN	denotes	ACD
T3860	16191-16197	NN	denotes	domain
T3863	16197-16199	,	denotes	,
T3864	16199-16201	CC	denotes	or
T3865	16202-16211	JJ	denotes	different
T3866	16212-16217	NNS	denotes	cells
T3867	16218-16222	VBN	denotes	used
T3868	16222-16223	.	denotes	.
T3869	16223-16411	sentence	denotes	In current study, the Acdp1 C-terminus antibody only recognizes Acdp1 in brain tissue extracts as well as in HEK293, 3T3 and PC12 cell lysates, suggesting the specificity of the antibody.
T3870	16224-16226	IN	denotes	In
T3872	16227-16234	JJ	denotes	current
T3873	16235-16240	NN	denotes	study
T3874	16240-16242	,	denotes	,
T3875	16242-16245	DT	denotes	the
T3877	16246-16251	NN	denotes	Acdp1
T3878	16252-16253	NN	denotes	C
T3880	16253-16254	HYPH	denotes	-
T3879	16254-16262	NN	denotes	terminus
T3876	16263-16271	NN	denotes	antibody
T3881	16272-16276	RB	denotes	only
T3871	16277-16287	VBZ	denotes	recognizes
T3882	16288-16293	NN	denotes	Acdp1
T3883	16294-16296	IN	denotes	in
T3884	16297-16302	NN	denotes	brain
T3885	16303-16309	NN	denotes	tissue
T3886	16310-16318	NNS	denotes	extracts
T3887	16319-16321	RB	denotes	as
T3889	16322-16326	RB	denotes	well
T3888	16327-16329	IN	denotes	as
T3890	16330-16332	IN	denotes	in
T3891	16333-16339	NN	denotes	HEK293
T3893	16339-16341	,	denotes	,
T3894	16341-16344	NN	denotes	3T3
T3895	16345-16348	CC	denotes	and
T3896	16349-16353	NN	denotes	PC12
T3897	16354-16358	NN	denotes	cell
T3892	16359-16366	NNS	denotes	lysates
T3898	16366-16368	,	denotes	,
T3899	16368-16378	VBG	denotes	suggesting
T3900	16379-16382	DT	denotes	the
T3901	16383-16394	NN	denotes	specificity
T3902	16395-16397	IN	denotes	of
T3903	16398-16401	DT	denotes	the
T3904	16402-16410	NN	denotes	antibody
T3905	16410-16411	.	denotes	.
T3906	16411-16503	sentence	denotes	Our observations suggested that Acdp might be involved in ion transport in mammalian cells.
T3907	16412-16415	PRP$	denotes	Our
T3908	16416-16428	NNS	denotes	observations
T3909	16429-16438	VBD	denotes	suggested
T3910	16439-16443	IN	denotes	that
T3912	16444-16448	NN	denotes	Acdp
T3913	16449-16454	MD	denotes	might
T3914	16455-16457	VB	denotes	be
T3911	16458-16466	VBN	denotes	involved
T3915	16467-16469	IN	denotes	in
T3916	16470-16473	NN	denotes	ion
T3917	16474-16483	NN	denotes	transport
T3918	16484-16486	IN	denotes	in
T3919	16487-16496	JJ	denotes	mammalian
T3920	16497-16502	NNS	denotes	cells
T3921	16502-16503	.	denotes	.
T3922	16503-16609	sentence	denotes	However, more detailed functional studies are needed to demonstrate the real functions of these proteins.
T3923	16504-16511	RB	denotes	However
T3925	16511-16513	,	denotes	,
T3926	16513-16517	RBR	denotes	more
T3927	16518-16526	JJ	denotes	detailed
T3929	16527-16537	JJ	denotes	functional
T3928	16538-16545	NNS	denotes	studies
T3930	16546-16549	VBP	denotes	are
T3924	16550-16556	VBN	denotes	needed
T3931	16557-16559	TO	denotes	to
T3932	16560-16571	VB	denotes	demonstrate
T3933	16572-16575	DT	denotes	the
T3935	16576-16580	JJ	denotes	real
T3934	16581-16590	NNS	denotes	functions
T3936	16591-16593	IN	denotes	of
T3937	16594-16599	DT	denotes	these
T3938	16600-16608	NN	denotes	proteins
T3939	16608-16609	.	denotes	.
T4051	16623-16626	PRP$	denotes	Our
T4053	16627-16635	JJ	denotes	previous
T4052	16636-16640	NN	denotes	work
T4055	16641-16644	VBZ	denotes	has
T4054	16645-16654	VBN	denotes	described
T4056	16655-16660	JJ	denotes	human
T4058	16661-16665	NN	denotes	ACDP
T4057	16666-16671	NNS	denotes	genes
T4059	16672-16673	-LRB-	denotes	[
T4060	16673-16674	CD	denotes	1
T4061	16674-16675	-RRB-	denotes	]
T4062	16675-16676	.	denotes	.
T4063	16676-16944	sentence	denotes	The new information of the current work includes: 1). It is the first time to report the existence of multiple Acdp genes in other mammals in addition to human, while Acdp appears to be a single copy gene in lower organisms such as in C. elegans, yeasts and bacteria.
T4064	16677-16680	DT	denotes	The
T4066	16681-16684	JJ	denotes	new
T4065	16685-16696	NN	denotes	information
T4068	16697-16699	IN	denotes	of
T4069	16700-16703	DT	denotes	the
T4071	16704-16711	JJ	denotes	current
T4070	16712-16716	NN	denotes	work
T4067	16717-16725	VBZ	denotes	includes
T4073	16725-16727	:	denotes	:
T4074	16727-16728	LS	denotes	1
T4075	16728-16729	-RRB-	denotes	)
T4076	16729-16730	,	denotes	.
T4077	16731-16733	PRP	denotes	It
T4072	16734-16736	VBZ	denotes	is
T4078	16737-16740	DT	denotes	the
T4080	16741-16746	JJ	denotes	first
T4079	16747-16751	NN	denotes	time
T4081	16752-16754	TO	denotes	to
T4082	16755-16761	VB	denotes	report
T4083	16762-16765	DT	denotes	the
T4084	16766-16775	NN	denotes	existence
T4085	16776-16778	IN	denotes	of
T4086	16779-16787	JJ	denotes	multiple
T4088	16788-16792	NN	denotes	Acdp
T4087	16793-16798	NNS	denotes	genes
T4089	16799-16801	IN	denotes	in
T4090	16802-16807	JJ	denotes	other
T4091	16808-16815	NNS	denotes	mammals
T4092	16816-16818	IN	denotes	in
T4093	16819-16827	NN	denotes	addition
T4094	16828-16830	IN	denotes	to
T4095	16831-16836	NN	denotes	human
T4096	16836-16838	,	denotes	,
T4097	16838-16843	IN	denotes	while
T4099	16844-16848	NN	denotes	Acdp
T4098	16849-16856	VBZ	denotes	appears
T4100	16857-16859	TO	denotes	to
T4101	16860-16862	VB	denotes	be
T4102	16863-16864	DT	denotes	a
T4104	16865-16871	JJ	denotes	single
T4105	16872-16876	NN	denotes	copy
T4103	16877-16881	NN	denotes	gene
T4106	16882-16884	IN	denotes	in
T4107	16885-16890	JJR	denotes	lower
T4108	16891-16900	NNS	denotes	organisms
T4109	16901-16905	JJ	denotes	such
T4110	16906-16908	IN	denotes	as
T4111	16909-16911	IN	denotes	in
T4112	16912-16914	NNP	denotes	C.
T4113	16915-16922	NNP	denotes	elegans
T4114	16922-16924	,	denotes	,
T4115	16924-16930	NNS	denotes	yeasts
T4116	16931-16934	CC	denotes	and
T4117	16935-16943	NNS	denotes	bacteria
T4118	16943-16944	.	denotes	.
T4119	16944-17572	sentence	denotes	We have also suggested the evolutionary relationships of Acdp genes in different species (phylogenetic tree); 2). Molecular cloning and characterization of murine Acdp gene family are essential for study of this novel gene family in animal model, e.g. for generation of knockout or transgenic models; 3). We have demonstrated both DNA and amino acid conservations between mouse and human for each Acdp gene and the whole Acdp gene family, which provide important information for the possibility of functional redundancy or overlap between Acdp members; 4). We have generated antibodies specific for Acdp1 and all Acdp proteins.
T4120	16945-16947	PRP	denotes	We
T4122	16948-16952	VBP	denotes	have
T4123	16953-16957	RB	denotes	also
T4121	16958-16967	VBN	denotes	suggested
T4125	16968-16971	DT	denotes	the
T4127	16972-16984	JJ	denotes	evolutionary
T4126	16985-16998	NNS	denotes	relationships
T4128	16999-17001	IN	denotes	of
T4129	17002-17006	NN	denotes	Acdp
T4130	17007-17012	NNS	denotes	genes
T4131	17013-17015	IN	denotes	in
T4132	17016-17025	JJ	denotes	different
T4133	17026-17033	NNS	denotes	species
T4134	17034-17035	-LRB-	denotes	(
T4136	17035-17047	JJ	denotes	phylogenetic
T4135	17048-17052	NN	denotes	tree
T4137	17052-17053	-RRB-	denotes	)
T4138	17053-17054	:	denotes	;
T4139	17055-17056	LS	denotes	2
T4141	17056-17057	-RRB-	denotes	)
T4142	17057-17058	,	denotes	.
T4143	17059-17068	JJ	denotes	Molecular
T4144	17069-17076	NN	denotes	cloning
T4145	17077-17080	CC	denotes	and
T4146	17081-17097	NN	denotes	characterization
T4147	17098-17100	IN	denotes	of
T4148	17101-17107	JJ	denotes	murine
T4150	17108-17112	NN	denotes	Acdp
T4151	17113-17117	NN	denotes	gene
T4149	17118-17124	NN	denotes	family
T4140	17125-17128	VBP	denotes	are
T4152	17129-17138	JJ	denotes	essential
T4153	17139-17142	IN	denotes	for
T4154	17143-17148	NN	denotes	study
T4155	17149-17151	IN	denotes	of
T4156	17152-17156	DT	denotes	this
T4158	17157-17162	JJ	denotes	novel
T4159	17163-17167	NN	denotes	gene
T4157	17168-17174	NN	denotes	family
T4160	17175-17177	IN	denotes	in
T4161	17178-17184	NN	denotes	animal
T4162	17185-17190	NN	denotes	model
T4163	17190-17192	,	denotes	,
T4164	17192-17196	FW	denotes	e.g.
T4165	17197-17200	IN	denotes	for
T4166	17201-17211	NN	denotes	generation
T4167	17212-17214	IN	denotes	of
T4168	17215-17223	NN	denotes	knockout
T4170	17224-17226	CC	denotes	or
T4171	17227-17237	JJ	denotes	transgenic
T4169	17238-17244	NNS	denotes	models
T4172	17244-17245	:	denotes	;
T4173	17246-17247	LS	denotes	3
T4175	17247-17248	-RRB-	denotes	)
T4176	17248-17249	,	denotes	.
T4177	17250-17252	PRP	denotes	We
T4178	17253-17257	VBP	denotes	have
T4174	17258-17270	VBN	denotes	demonstrated
T4179	17271-17275	CC	denotes	both
T4180	17276-17279	NN	denotes	DNA
T4182	17280-17283	CC	denotes	and
T4183	17284-17289	NN	denotes	amino
T4184	17290-17294	NN	denotes	acid
T4181	17295-17308	NNS	denotes	conservations
T4185	17309-17316	IN	denotes	between
T4186	17317-17322	NN	denotes	mouse
T4187	17323-17326	CC	denotes	and
T4188	17327-17332	NN	denotes	human
T4189	17333-17336	IN	denotes	for
T4190	17337-17341	DT	denotes	each
T4192	17342-17346	NN	denotes	Acdp
T4191	17347-17351	NN	denotes	gene
T4193	17352-17355	CC	denotes	and
T4194	17356-17359	DT	denotes	the
T4196	17360-17365	JJ	denotes	whole
T4197	17366-17370	NN	denotes	Acdp
T4198	17371-17375	NN	denotes	gene
T4195	17376-17382	NN	denotes	family
T4199	17382-17384	,	denotes	,
T4200	17384-17389	WDT	denotes	which
T4201	17390-17397	VBP	denotes	provide
T4202	17398-17407	JJ	denotes	important
T4203	17408-17419	NN	denotes	information
T4204	17420-17423	IN	denotes	for
T4205	17424-17427	DT	denotes	the
T4206	17428-17439	NN	denotes	possibility
T4207	17440-17442	IN	denotes	of
T4208	17443-17453	JJ	denotes	functional
T4209	17454-17464	NN	denotes	redundancy
T4210	17465-17467	CC	denotes	or
T4211	17468-17475	NN	denotes	overlap
T4212	17476-17483	IN	denotes	between
T4213	17484-17488	NN	denotes	Acdp
T4214	17489-17496	NNS	denotes	members
T4215	17496-17497	:	denotes	;
T4216	17498-17499	LS	denotes	4
T4217	17499-17500	-RRB-	denotes	)
T4218	17500-17501	,	denotes	.
T4219	17502-17504	PRP	denotes	We
T4220	17505-17509	VBP	denotes	have
T4124	17510-17519	VBN	denotes	generated
T4221	17520-17530	NNS	denotes	antibodies
T4222	17531-17539	JJ	denotes	specific
T4223	17540-17543	IN	denotes	for
T4224	17544-17549	NN	denotes	Acdp1
T4225	17550-17553	CC	denotes	and
T4226	17554-17557	DT	denotes	all
T4228	17558-17562	NN	denotes	Acdp
T4227	17563-17571	NN	denotes	proteins
T4229	17571-17572	.	denotes	.
T4230	17572-17643	sentence	denotes	The Acdp1 C-terminus antibody appears to specifically recognize Acdp1.
T4231	17573-17576	DT	denotes	The
T4233	17577-17582	NN	denotes	Acdp1
T4234	17583-17584	NN	denotes	C
T4236	17584-17585	HYPH	denotes	-
T4235	17585-17593	NN	denotes	terminus
T4232	17594-17602	NN	denotes	antibody
T4237	17603-17610	VBZ	denotes	appears
T4238	17611-17613	TO	denotes	to
T4240	17614-17626	RB	denotes	specifically
T4239	17627-17636	VB	denotes	recognize
T4241	17637-17642	NN	denotes	Acdp1
T4242	17642-17643	.	denotes	.
T4243	17643-17770	sentence	denotes	Using this antibody, we have demonstrated that Acdp1 is predominantly localized on the plasma membrane in hippocampus neurons.
T4244	17644-17649	VBG	denotes	Using
T4246	17650-17654	DT	denotes	this
T4247	17655-17663	NN	denotes	antibody
T4248	17663-17665	,	denotes	,
T4249	17665-17667	PRP	denotes	we
T4250	17668-17672	VBP	denotes	have
T4245	17673-17685	VBN	denotes	demonstrated
T4251	17686-17690	IN	denotes	that
T4253	17691-17696	NN	denotes	Acdp1
T4254	17697-17699	VBZ	denotes	is
T4255	17700-17713	RB	denotes	predominantly
T4252	17714-17723	VBN	denotes	localized
T4256	17724-17726	IN	denotes	on
T4257	17727-17730	DT	denotes	the
T4259	17731-17737	NN	denotes	plasma
T4258	17738-17746	NN	denotes	membrane
T4260	17747-17749	IN	denotes	in
T4261	17750-17761	NN	denotes	hippocampus
T4262	17762-17769	NNS	denotes	neurons
T4263	17769-17770	.	denotes	.
T4264	17770-17880	sentence	denotes	These results represent an important step towards the characterization of functions for the Acdp gene family.
T4265	17771-17776	DT	denotes	These
T4266	17777-17784	NNS	denotes	results
T4267	17785-17794	VBP	denotes	represent
T4268	17795-17797	DT	denotes	an
T4270	17798-17807	JJ	denotes	important
T4269	17808-17812	NN	denotes	step
T4271	17813-17820	IN	denotes	towards
T4272	17821-17824	DT	denotes	the
T4273	17825-17841	NN	denotes	characterization
T4274	17842-17844	IN	denotes	of
T4275	17845-17854	NNS	denotes	functions
T4276	17855-17858	IN	denotes	for
T4277	17859-17862	DT	denotes	the
T4279	17863-17867	NN	denotes	Acdp
T4280	17868-17872	NN	denotes	gene
T4278	17873-17879	NN	denotes	family
T4281	17879-17880	.	denotes	.
T4392	17891-17895	NN	denotes	cDNA
T4393	17896-17903	NN	denotes	cloning
T4394	17903-18017	sentence	denotes	cDNA cloning of the Acdp gene family was performed based on human homologue sequences as previously reported [2].
T4395	17904-17908	NN	denotes	cDNA
T4396	17909-17916	NN	denotes	cloning
T4398	17917-17919	IN	denotes	of
T4399	17920-17923	DT	denotes	the
T4401	17924-17928	NN	denotes	Acdp
T4402	17929-17933	NN	denotes	gene
T4400	17934-17940	NN	denotes	family
T4403	17941-17944	VBD	denotes	was
T4397	17945-17954	VBN	denotes	performed
T4404	17955-17960	VBN	denotes	based
T4405	17961-17963	IN	denotes	on
T4406	17964-17969	JJ	denotes	human
T4408	17970-17979	NN	denotes	homologue
T4407	17980-17989	NNS	denotes	sequences
T4409	17990-17992	IN	denotes	as
T4411	17993-18003	RB	denotes	previously
T4410	18004-18012	VBN	denotes	reported
T4412	18013-18014	-LRB-	denotes	[
T4413	18014-18015	CD	denotes	2
T4414	18015-18016	-RRB-	denotes	]
T4415	18016-18017	.	denotes	.
T4416	18017-18168	sentence	denotes	Briefly, the human ACDP cDNAs and predicted AA sequences were used to search the mouse EST database for EST markers corresponding to each Acdp member.
T4417	18018-18025	RB	denotes	Briefly
T4419	18025-18027	,	denotes	,
T4420	18027-18030	DT	denotes	the
T4422	18031-18036	JJ	denotes	human
T4423	18037-18041	NN	denotes	ACDP
T4421	18042-18047	NNS	denotes	cDNAs
T4424	18048-18051	CC	denotes	and
T4425	18052-18061	VBN	denotes	predicted
T4427	18062-18064	NN	denotes	AA
T4426	18065-18074	NNS	denotes	sequences
T4428	18075-18079	VBD	denotes	were
T4418	18080-18084	VBN	denotes	used
T4429	18085-18087	TO	denotes	to
T4430	18088-18094	VB	denotes	search
T4431	18095-18098	DT	denotes	the
T4433	18099-18104	NN	denotes	mouse
T4434	18105-18108	NN	denotes	EST
T4432	18109-18117	NN	denotes	database
T4435	18118-18121	IN	denotes	for
T4436	18122-18125	NN	denotes	EST
T4437	18126-18133	NNS	denotes	markers
T4438	18134-18147	VBG	denotes	corresponding
T4439	18148-18150	IN	denotes	to
T4440	18151-18155	DT	denotes	each
T4442	18156-18160	NN	denotes	Acdp
T4441	18161-18167	NN	denotes	member
T4443	18167-18168	.	denotes	.
T4444	18168-18407	sentence	denotes	A forward primer within the human ACDP 5' cDNA coding region (after start codon) and a mouse reverse primer from the mouse EST marker were used to amplify the homologue sequence from mouse cDNA at very low annealing temperature (45–50°C).
T4445	18169-18170	DT	denotes	A
T4447	18171-18178	JJ	denotes	forward
T4446	18179-18185	NN	denotes	primer
T4449	18186-18192	IN	denotes	within
T4450	18193-18196	DT	denotes	the
T4452	18197-18202	JJ	denotes	human
T4453	18203-18207	NN	denotes	ACDP
T4454	18208-18209	CD	denotes	5
T4455	18209-18210	SYM	denotes	'
T4456	18211-18215	NN	denotes	cDNA
T4457	18216-18222	NN	denotes	coding
T4451	18223-18229	NN	denotes	region
T4458	18230-18231	-LRB-	denotes	(
T4459	18231-18236	IN	denotes	after
T4460	18237-18242	NN	denotes	start
T4461	18243-18248	NN	denotes	codon
T4462	18248-18249	-RRB-	denotes	)
T4463	18250-18253	CC	denotes	and
T4464	18254-18255	DT	denotes	a
T4466	18256-18261	NN	denotes	mouse
T4467	18262-18269	JJ	denotes	reverse
T4465	18270-18276	NN	denotes	primer
T4468	18277-18281	IN	denotes	from
T4469	18282-18285	DT	denotes	the
T4471	18286-18291	NN	denotes	mouse
T4472	18292-18295	NN	denotes	EST
T4470	18296-18302	NN	denotes	marker
T4473	18303-18307	VBD	denotes	were
T4448	18308-18312	VBN	denotes	used
T4474	18313-18315	TO	denotes	to
T4475	18316-18323	VB	denotes	amplify
T4476	18324-18327	DT	denotes	the
T4478	18328-18337	NN	denotes	homologue
T4477	18338-18346	NN	denotes	sequence
T4479	18347-18351	IN	denotes	from
T4480	18352-18357	NN	denotes	mouse
T4481	18358-18362	NN	denotes	cDNA
T4482	18363-18365	IN	denotes	at
T4483	18366-18370	RB	denotes	very
T4484	18371-18374	JJ	denotes	low
T4486	18375-18384	VBG	denotes	annealing
T4485	18385-18396	NN	denotes	temperature
T4487	18397-18398	-LRB-	denotes	(
T4488	18398-18400	CD	denotes	45
T4490	18400-18401	SYM	denotes	–
T4489	18401-18403	CD	denotes	50
T4491	18403-18405	NN	denotes	°C
T4492	18405-18406	-RRB-	denotes	)
T4493	18406-18407	.	denotes	.
T4494	18407-18641	sentence	denotes	A nested PCR using an inside reverse primer from the mouse EST sequence and the same human forward primer was then carried out to amplify the specific mouse gene from the first round PCR products at high annealing temperature (62°C).
T4495	18408-18409	DT	denotes	A
T4497	18410-18416	JJ	denotes	nested
T4496	18417-18420	NN	denotes	PCR
T4499	18421-18426	VBG	denotes	using
T4500	18427-18429	DT	denotes	an
T4502	18430-18436	JJ	denotes	inside
T4503	18437-18444	JJ	denotes	reverse
T4501	18445-18451	NN	denotes	primer
T4504	18452-18456	IN	denotes	from
T4505	18457-18460	DT	denotes	the
T4507	18461-18466	NN	denotes	mouse
T4508	18467-18470	NN	denotes	EST
T4506	18471-18479	NN	denotes	sequence
T4509	18480-18483	CC	denotes	and
T4510	18484-18487	DT	denotes	the
T4512	18488-18492	JJ	denotes	same
T4513	18493-18498	JJ	denotes	human
T4514	18499-18506	JJ	denotes	forward
T4511	18507-18513	NN	denotes	primer
T4515	18514-18517	VBD	denotes	was
T4516	18518-18522	RB	denotes	then
T4498	18523-18530	VBN	denotes	carried
T4517	18531-18534	RP	denotes	out
T4518	18535-18537	TO	denotes	to
T4519	18538-18545	VB	denotes	amplify
T4520	18546-18549	DT	denotes	the
T4522	18550-18558	JJ	denotes	specific
T4523	18559-18564	NN	denotes	mouse
T4521	18565-18569	NN	denotes	gene
T4524	18570-18574	IN	denotes	from
T4525	18575-18578	DT	denotes	the
T4527	18579-18584	JJ	denotes	first
T4528	18585-18590	NN	denotes	round
T4529	18591-18594	NN	denotes	PCR
T4526	18595-18603	NNS	denotes	products
T4530	18604-18606	IN	denotes	at
T4531	18607-18611	JJ	denotes	high
T4533	18612-18621	VBG	denotes	annealing
T4532	18622-18633	NN	denotes	temperature
T4534	18634-18635	-LRB-	denotes	(
T4535	18635-18637	CD	denotes	62
T4536	18637-18639	NN	denotes	°C
T4537	18639-18640	-RRB-	denotes	)
T4538	18640-18641	.	denotes	.
T4539	18641-18754	sentence	denotes	The expected PCR products were directly excised from agarose gel and sequenced by an ABI377 automatic sequencer.
T4540	18642-18645	DT	denotes	The
T4542	18646-18654	JJ	denotes	expected
T4543	18655-18658	NN	denotes	PCR
T4541	18659-18667	NNS	denotes	products
T4545	18668-18672	VBD	denotes	were
T4546	18673-18681	RB	denotes	directly
T4544	18682-18689	VBN	denotes	excised
T4547	18690-18694	IN	denotes	from
T4548	18695-18702	NN	denotes	agarose
T4549	18703-18706	NN	denotes	gel
T4550	18707-18710	CC	denotes	and
T4551	18711-18720	VBN	denotes	sequenced
T4552	18721-18723	IN	denotes	by
T4553	18724-18726	DT	denotes	an
T4555	18727-18733	NN	denotes	ABI377
T4556	18734-18743	JJ	denotes	automatic
T4554	18744-18753	NN	denotes	sequencer
T4557	18753-18754	.	denotes	.
T4558	18754-18885	sentence	denotes	The sequence was further confirmed using a forward primer from newly identified sequence and a reverse primer from known sequence.
T4559	18755-18758	DT	denotes	The
T4560	18759-18767	NN	denotes	sequence
T4562	18768-18771	VBD	denotes	was
T4563	18772-18779	RB	denotes	further
T4561	18780-18789	VBN	denotes	confirmed
T4564	18790-18795	VBG	denotes	using
T4565	18796-18797	DT	denotes	a
T4567	18798-18805	JJ	denotes	forward
T4566	18806-18812	NN	denotes	primer
T4568	18813-18817	IN	denotes	from
T4569	18818-18823	RB	denotes	newly
T4570	18824-18834	VBN	denotes	identified
T4571	18835-18843	NN	denotes	sequence
T4572	18844-18847	CC	denotes	and
T4573	18848-18849	DT	denotes	a
T4575	18850-18857	JJ	denotes	reverse
T4574	18858-18864	NN	denotes	primer
T4576	18865-18869	IN	denotes	from
T4577	18870-18875	JJ	denotes	known
T4578	18876-18884	NN	denotes	sequence
T4579	18884-18885	.	denotes	.
T4580	18885-19025	sentence	denotes	Once most of the coding sequences were identified, partial sequence of exon 1 and the 5' UTR sequences were obtained by BAC DNA sequencing.
T4581	18886-18890	IN	denotes	Once
T4583	18891-18895	JJS	denotes	most
T4584	18896-18898	IN	denotes	of
T4585	18899-18902	DT	denotes	the
T4587	18903-18909	VBG	denotes	coding
T4586	18910-18919	NNS	denotes	sequences
T4588	18920-18924	VBD	denotes	were
T4582	18925-18935	VBN	denotes	identified
T4590	18935-18937	,	denotes	,
T4591	18937-18944	JJ	denotes	partial
T4592	18945-18953	NN	denotes	sequence
T4593	18954-18956	IN	denotes	of
T4594	18957-18961	NN	denotes	exon
T4596	18962-18963	CD	denotes	1
T4597	18964-18967	CC	denotes	and
T4598	18968-18971	DT	denotes	the
T4600	18972-18973	CD	denotes	5
T4601	18973-18974	SYM	denotes	'
T4599	18975-18978	NN	denotes	UTR
T4595	18979-18988	NNS	denotes	sequences
T4602	18989-18993	VBD	denotes	were
T4589	18994-19002	VBN	denotes	obtained
T4603	19003-19005	IN	denotes	by
T4604	19006-19009	NN	denotes	BAC
T4606	19010-19013	NN	denotes	DNA
T4605	19014-19024	NN	denotes	sequencing
T4607	19024-19025	.	denotes	.
T4647	19027-19035	NNP	denotes	Northern
T4648	19036-19040	NN	denotes	blot
T4649	19041-19049	NNS	denotes	analyses
T4650	19049-19154	sentence	denotes	Multiple Choice Northern Blot filters containing 12 different mouse tissues were purchased from Origene.
T4651	19050-19058	JJ	denotes	Multiple
T4653	19059-19065	NN	denotes	Choice
T4654	19066-19074	NNP	denotes	Northern
T4655	19075-19079	NN	denotes	Blot
T4652	19080-19087	NNS	denotes	filters
T4657	19088-19098	VBG	denotes	containing
T4658	19099-19101	CD	denotes	12
T4660	19102-19111	JJ	denotes	different
T4661	19112-19117	NN	denotes	mouse
T4659	19118-19125	NNS	denotes	tissues
T4662	19126-19130	VBD	denotes	were
T4656	19131-19140	VBN	denotes	purchased
T4663	19141-19145	IN	denotes	from
T4664	19146-19153	NNP	denotes	Origene
T4665	19153-19154	.	denotes	.
T4666	19154-19365	sentence	denotes	The filters were probed for each Acdp gene with a PCR fragment (around 350 bp) from last exon and the 3' untranslated region labeled with α-32P dCTP using the random primer extension system (Life Technologies).
T4667	19155-19158	DT	denotes	The
T4668	19159-19166	NNS	denotes	filters
T4670	19167-19171	VBD	denotes	were
T4669	19172-19178	VBN	denotes	probed
T4671	19179-19182	IN	denotes	for
T4672	19183-19187	DT	denotes	each
T4674	19188-19192	NN	denotes	Acdp
T4673	19193-19197	NN	denotes	gene
T4675	19198-19202	IN	denotes	with
T4676	19203-19204	DT	denotes	a
T4678	19205-19208	NN	denotes	PCR
T4677	19209-19217	NN	denotes	fragment
T4679	19218-19219	-LRB-	denotes	(
T4681	19219-19225	IN	denotes	around
T4682	19226-19229	CD	denotes	350
T4680	19230-19232	NN	denotes	bp
T4683	19232-19233	-RRB-	denotes	)
T4684	19234-19238	IN	denotes	from
T4685	19239-19243	JJ	denotes	last
T4686	19244-19248	NN	denotes	exon
T4687	19249-19252	CC	denotes	and
T4688	19253-19256	DT	denotes	the
T4689	19257-19258	CD	denotes	3
T4691	19258-19259	SYM	denotes	'
T4692	19260-19272	JJ	denotes	untranslated
T4690	19273-19279	NN	denotes	region
T4693	19280-19287	VBN	denotes	labeled
T4694	19288-19292	IN	denotes	with
T4695	19293-19294	NN	denotes	α
T4697	19294-19295	HYPH	denotes	-
T4696	19295-19298	NN	denotes	32P
T4698	19299-19303	NN	denotes	dCTP
T4699	19304-19309	VBG	denotes	using
T4700	19310-19313	DT	denotes	the
T4702	19314-19320	JJ	denotes	random
T4703	19321-19327	NN	denotes	primer
T4704	19328-19337	NN	denotes	extension
T4701	19338-19344	NN	denotes	system
T4705	19345-19346	-LRB-	denotes	(
T4707	19346-19350	NNP	denotes	Life
T4706	19351-19363	NNP	denotes	Technologies
T4708	19363-19364	-RRB-	denotes	)
T4709	19364-19365	.	denotes	.
T4710	19365-19408	sentence	denotes	Hybridizations were carried out overnight.
T4711	19366-19380	NNS	denotes	Hybridizations
T4713	19381-19385	VBD	denotes	were
T4712	19386-19393	VBN	denotes	carried
T4714	19394-19397	RP	denotes	out
T4715	19398-19407	RB	denotes	overnight
T4716	19407-19408	.	denotes	.
T4717	19408-19584	sentence	denotes	The filters were washed twice with washing buffer I (2×SSC, 0.1% SDS) at 42°C for 15-min, and then washed twice with washing buffer II (0.25×SSC, 0.1% SDS) at 65°C for 15-min.
T4718	19409-19412	DT	denotes	The
T4719	19413-19420	NNS	denotes	filters
T4721	19421-19425	VBD	denotes	were
T4720	19426-19432	VBN	denotes	washed
T4722	19433-19438	RB	denotes	twice
T4723	19439-19443	IN	denotes	with
T4724	19444-19451	VBG	denotes	washing
T4725	19452-19458	NN	denotes	buffer
T4726	19459-19460	CD	denotes	I
T4727	19461-19462	-LRB-	denotes	(
T4729	19462-19463	CD	denotes	2
T4731	19463-19464	SYM	denotes	×
T4730	19464-19467	NN	denotes	SSC
T4732	19467-19469	,	denotes	,
T4733	19469-19472	CD	denotes	0.1
T4734	19472-19473	NN	denotes	%
T4728	19474-19477	NN	denotes	SDS
T4735	19477-19478	-RRB-	denotes	)
T4736	19479-19481	IN	denotes	at
T4737	19482-19484	CD	denotes	42
T4738	19484-19486	NN	denotes	°C
T4739	19487-19490	IN	denotes	for
T4740	19491-19493	CD	denotes	15
T4742	19493-19494	HYPH	denotes	-
T4741	19494-19497	NN	denotes	min
T4743	19497-19499	,	denotes	,
T4744	19499-19502	CC	denotes	and
T4745	19503-19507	RB	denotes	then
T4746	19508-19514	VBD	denotes	washed
T4747	19515-19520	RB	denotes	twice
T4748	19521-19525	IN	denotes	with
T4749	19526-19533	NN	denotes	washing
T4750	19534-19540	NN	denotes	buffer
T4751	19541-19543	CD	denotes	II
T4752	19544-19545	-LRB-	denotes	(
T4754	19545-19549	CD	denotes	0.25
T4756	19549-19550	SYM	denotes	×
T4755	19550-19553	NN	denotes	SSC
T4757	19553-19555	,	denotes	,
T4758	19555-19558	CD	denotes	0.1
T4759	19558-19559	NN	denotes	%
T4753	19560-19563	NN	denotes	SDS
T4760	19563-19564	-RRB-	denotes	)
T4761	19565-19567	IN	denotes	at
T4762	19568-19570	CD	denotes	65
T4763	19570-19572	NN	denotes	°C
T4764	19573-19576	IN	denotes	for
T4765	19577-19579	CD	denotes	15
T4767	19579-19580	HYPH	denotes	-
T4766	19580-19583	NN	denotes	min
T4768	19583-19584	.	denotes	.
T4769	19584-19658	sentence	denotes	Washed filters were exposed to X-ray films for overnight or longer [3,4].
T4770	19585-19591	JJ	denotes	Washed
T4771	19592-19599	NNS	denotes	filters
T4773	19600-19604	VBD	denotes	were
T4772	19605-19612	VBN	denotes	exposed
T4774	19613-19615	IN	denotes	to
T4775	19616-19621	NN	denotes	X-ray
T4776	19622-19627	NNS	denotes	films
T4777	19628-19631	IN	denotes	for
T4778	19632-19641	NN	denotes	overnight
T4779	19642-19644	CC	denotes	or
T4780	19645-19651	RBR	denotes	longer
T4781	19652-19653	-LRB-	denotes	[
T4783	19653-19654	CD	denotes	3
T4784	19654-19655	,	denotes	,
T4782	19655-19656	CD	denotes	4
T4785	19656-19657	-RRB-	denotes	]
T4786	19657-19658	.	denotes	.
T4823	19660-19668	NN	denotes	Antibody
T4824	19669-19679	NN	denotes	production
T4825	19680-19683	CC	denotes	and
T4826	19684-19691	NNP	denotes	Western
T4827	19692-19696	NN	denotes	blot
T4828	19697-19705	NNS	denotes	analyses
T4829	19705-19931	sentence	denotes	Peptides linked to KLH (keyhole limpet hemacyanin) from Acdp1 N- and C-terminals and the conserved domain (ACD) were used for generation of antibodies specifically for Acdp1 and all Acdp members as reported, respectively [5].
T4830	19706-19714	NNS	denotes	Peptides
T4832	19715-19721	VBN	denotes	linked
T4833	19722-19724	IN	denotes	to
T4834	19725-19728	NN	denotes	KLH
T4835	19729-19730	-LRB-	denotes	(
T4836	19730-19737	NN	denotes	keyhole
T4838	19738-19744	NN	denotes	limpet
T4837	19745-19755	NN	denotes	hemacyanin
T4839	19755-19756	-RRB-	denotes	)
T4840	19757-19761	IN	denotes	from
T4841	19762-19767	NN	denotes	Acdp1
T4843	19768-19769	NN	denotes	N
T4844	19769-19770	HYPH	denotes	-
T4845	19771-19774	CC	denotes	and
T4846	19775-19776	NN	denotes	C
T4847	19776-19777	HYPH	denotes	-
T4842	19777-19786	NNS	denotes	terminals
T4848	19787-19790	CC	denotes	and
T4849	19791-19794	DT	denotes	the
T4851	19795-19804	VBN	denotes	conserved
T4850	19805-19811	NN	denotes	domain
T4852	19812-19813	-LRB-	denotes	(
T4853	19813-19816	NN	denotes	ACD
T4854	19816-19817	-RRB-	denotes	)
T4855	19818-19822	VBD	denotes	were
T4831	19823-19827	VBN	denotes	used
T4856	19828-19831	IN	denotes	for
T4857	19832-19842	NN	denotes	generation
T4858	19843-19845	IN	denotes	of
T4859	19846-19856	NNS	denotes	antibodies
T4860	19857-19869	RB	denotes	specifically
T4861	19870-19873	IN	denotes	for
T4862	19874-19879	NN	denotes	Acdp1
T4863	19880-19883	CC	denotes	and
T4864	19884-19887	DT	denotes	all
T4866	19888-19892	NN	denotes	Acdp
T4865	19893-19900	NNS	denotes	members
T4867	19901-19903	IN	denotes	as
T4868	19904-19912	VBN	denotes	reported
T4869	19912-19914	,	denotes	,
T4870	19914-19926	RB	denotes	respectively
T4871	19927-19928	-LRB-	denotes	[
T4872	19928-19929	CD	denotes	5
T4873	19929-19930	-RRB-	denotes	]
T4874	19930-19931	.	denotes	.
T4875	19931-20010	sentence	denotes	Western blots were carried out Using ECL (PIERCE) as described previously [1].
T4876	19932-19939	NNP	denotes	Western
T4877	19940-19945	NNS	denotes	blots
T4879	19946-19950	VBD	denotes	were
T4878	19951-19958	VBN	denotes	carried
T4880	19959-19962	RP	denotes	out
T4881	19963-19968	VBG	denotes	Using
T4882	19969-19972	NN	denotes	ECL
T4883	19973-19974	-LRB-	denotes	(
T4884	19974-19980	NN	denotes	PIERCE
T4885	19980-19981	-RRB-	denotes	)
T4886	19982-19984	IN	denotes	as
T4887	19985-19994	VBN	denotes	described
T4888	19995-20005	RB	denotes	previously
T4889	20006-20007	-LRB-	denotes	[
T4890	20007-20008	CD	denotes	1
T4891	20008-20009	-RRB-	denotes	]
T4892	20009-20010	.	denotes	.
T4893	20010-20172	sentence	denotes	The membranes were washed extensively after incubation with primary and secondary antibodies and were then developed with X-ray films with optimal exposure time.
T4894	20011-20014	DT	denotes	The
T4895	20015-20024	NNS	denotes	membranes
T4897	20025-20029	VBD	denotes	were
T4896	20030-20036	VBN	denotes	washed
T4898	20037-20048	RB	denotes	extensively
T4899	20049-20054	IN	denotes	after
T4900	20055-20065	NN	denotes	incubation
T4901	20066-20070	IN	denotes	with
T4902	20071-20078	JJ	denotes	primary
T4904	20079-20082	CC	denotes	and
T4905	20083-20092	JJ	denotes	secondary
T4903	20093-20103	NNS	denotes	antibodies
T4906	20104-20107	CC	denotes	and
T4907	20108-20112	VBD	denotes	were
T4909	20113-20117	RB	denotes	then
T4908	20118-20127	VBN	denotes	developed
T4910	20128-20132	IN	denotes	with
T4911	20133-20138	NN	denotes	X-ray
T4912	20139-20144	NNS	denotes	films
T4913	20145-20149	IN	denotes	with
T4914	20150-20157	JJ	denotes	optimal
T4916	20158-20166	NN	denotes	exposure
T4915	20167-20171	NN	denotes	time
T4917	20171-20172	.	denotes	.
T4941	20174-20184	NN	denotes	Chromosome
T4942	20185-20197	NN	denotes	localization
T4943	20197-20318	sentence	denotes	The T31 mouse radiation hybrid panel from Research Genetics was used to map the chromosome location of each Acdp member.
T4944	20198-20201	DT	denotes	The
T4946	20202-20205	NN	denotes	T31
T4947	20206-20211	NN	denotes	mouse
T4948	20212-20221	NN	denotes	radiation
T4949	20222-20228	NN	denotes	hybrid
T4945	20229-20234	NN	denotes	panel
T4951	20235-20239	IN	denotes	from
T4952	20240-20248	NNP	denotes	Research
T4953	20249-20257	NNP	denotes	Genetics
T4954	20258-20261	VBD	denotes	was
T4950	20262-20266	VBN	denotes	used
T4955	20267-20269	TO	denotes	to
T4956	20270-20273	VB	denotes	map
T4957	20274-20277	DT	denotes	the
T4959	20278-20288	NN	denotes	chromosome
T4958	20289-20297	NN	denotes	location
T4960	20298-20300	IN	denotes	of
T4961	20301-20305	DT	denotes	each
T4963	20306-20310	NN	denotes	Acdp
T4962	20311-20317	NN	denotes	member
T4964	20317-20318	.	denotes	.
T4965	20318-20442	sentence	denotes	Primers from 3' UTR of each Acdp member were used to amplify the 100 radiation hybrid clones representing the mouse genome.
T4966	20319-20326	NNS	denotes	Primers
T4968	20327-20331	IN	denotes	from
T4969	20332-20333	CD	denotes	3
T4971	20333-20334	SYM	denotes	'
T4970	20335-20338	NN	denotes	UTR
T4972	20339-20341	IN	denotes	of
T4973	20342-20346	DT	denotes	each
T4975	20347-20351	NN	denotes	Acdp
T4974	20352-20358	NN	denotes	member
T4976	20359-20363	VBD	denotes	were
T4967	20364-20368	VBN	denotes	used
T4977	20369-20371	TO	denotes	to
T4978	20372-20379	VB	denotes	amplify
T4979	20380-20383	DT	denotes	the
T4981	20384-20387	CD	denotes	100
T4982	20388-20397	NN	denotes	radiation
T4983	20398-20404	JJ	denotes	hybrid
T4980	20405-20411	NNS	denotes	clones
T4984	20412-20424	VBG	denotes	representing
T4985	20425-20428	DT	denotes	the
T4987	20429-20434	NN	denotes	mouse
T4986	20435-20441	NN	denotes	genome
T4988	20441-20442	.	denotes	.
T4989	20442-20538	sentence	denotes	The data were submitted to the Jackson Laboratory Mouse Radiation Hybrid Database for analysis.
T4990	20443-20446	DT	denotes	The
T4991	20447-20451	NNS	denotes	data
T4993	20452-20456	VBD	denotes	were
T4992	20457-20466	VBN	denotes	submitted
T4994	20467-20469	IN	denotes	to
T4995	20470-20473	DT	denotes	the
T4997	20474-20481	NNP	denotes	Jackson
T4998	20482-20492	NNP	denotes	Laboratory
T4999	20493-20498	NNP	denotes	Mouse
T5001	20499-20508	NNP	denotes	Radiation
T5000	20509-20515	NNP	denotes	Hybrid
T4996	20516-20524	NNP	denotes	Database
T5002	20525-20528	IN	denotes	for
T5003	20529-20537	NN	denotes	analysis
T5004	20537-20538	.	denotes	.
T5023	20540-20548	NN	denotes	Sequence
T5024	20549-20557	NNS	denotes	analyses
T5025	20557-20632	sentence	denotes	Sequence assembly was performed with program Sequencher (Gene Codes Corp).
T5026	20558-20566	NN	denotes	Sequence
T5027	20567-20575	NN	denotes	assembly
T5029	20576-20579	VBD	denotes	was
T5028	20580-20589	VBN	denotes	performed
T5030	20590-20594	IN	denotes	with
T5031	20595-20602	NN	denotes	program
T5032	20603-20613	NN	denotes	Sequencher
T5033	20614-20615	-LRB-	denotes	(
T5035	20615-20619	NNP	denotes	Gene
T5036	20620-20625	NNPS	denotes	Codes
T5034	20626-20630	NNP	denotes	Corp
T5037	20630-20631	-RRB-	denotes	)
T5038	20631-20632	.	denotes	.
T5039	20632-20735	sentence	denotes	Protein and DNA homology searches were carried out with tblastn, tblastx, blastp and blastn programs .
T5040	20633-20640	NN	denotes	Protein
T5042	20641-20644	CC	denotes	and
T5043	20645-20648	NN	denotes	DNA
T5041	20649-20657	NN	denotes	homology
T5044	20658-20666	NNS	denotes	searches
T5046	20667-20671	VBD	denotes	were
T5045	20672-20679	VBN	denotes	carried
T5047	20680-20683	RP	denotes	out
T5048	20684-20688	IN	denotes	with
T5049	20689-20696	NN	denotes	tblastn
T5051	20696-20698	,	denotes	,
T5052	20698-20705	NN	denotes	tblastx
T5053	20705-20707	,	denotes	,
T5054	20707-20713	NN	denotes	blastp
T5055	20714-20717	CC	denotes	and
T5056	20718-20724	NN	denotes	blastn
T5050	20725-20733	NNS	denotes	programs
T5057	20734-20735	.	denotes	.
T5058	20735-20826	sentence	denotes	Multiple sequence alignments were performed with GeneDoc and pairwise sequence alignment .
T5059	20736-20744	JJ	denotes	Multiple
T5061	20745-20753	NN	denotes	sequence
T5060	20754-20764	NNS	denotes	alignments
T5063	20765-20769	VBD	denotes	were
T5062	20770-20779	VBN	denotes	performed
T5064	20780-20784	IN	denotes	with
T5065	20785-20792	NN	denotes	GeneDoc
T5067	20793-20796	CC	denotes	and
T5068	20797-20805	JJ	denotes	pairwise
T5069	20806-20814	NN	denotes	sequence
T5066	20815-20824	NN	denotes	alignment
T5070	20825-20826	.	denotes	.
T5071	20826-20994	sentence	denotes	Multiple programs including BCM Search Launcher , ProfileScan , sequence motif search , ExPASy and 3Dpssm were used for searching sequence features of known protein.
T5072	20827-20835	JJ	denotes	Multiple
T5073	20836-20844	NNS	denotes	programs
T5075	20845-20854	VBG	denotes	including
T5076	20855-20858	NN	denotes	BCM
T5078	20859-20865	NN	denotes	Search
T5077	20866-20874	NN	denotes	Launcher
T5079	20875-20877	,	denotes	,
T5080	20877-20888	NN	denotes	ProfileScan
T5081	20889-20891	,	denotes	,
T5082	20891-20899	NN	denotes	sequence
T5084	20900-20905	NN	denotes	motif
T5083	20906-20912	NN	denotes	search
T5085	20913-20915	,	denotes	,
T5086	20915-20921	NN	denotes	ExPASy
T5087	20923-20926	CC	denotes	and
T5088	20927-20933	NN	denotes	3Dpssm
T5089	20935-20939	VBD	denotes	were
T5074	20940-20944	VBN	denotes	used
T5090	20945-20948	IN	denotes	for
T5091	20949-20958	VBG	denotes	searching
T5092	20959-20967	NN	denotes	sequence
T5093	20968-20976	NNS	denotes	features
T5094	20977-20979	IN	denotes	of
T5095	20980-20985	JJ	denotes	known
T5096	20986-20993	NN	denotes	protein
T5097	20993-20994	.	denotes	.
T5098	20994-21148	sentence	denotes	Phylogenetic tree was constructed by Clustalw program (version 1.81) using UPGMA (Unweighted Pair Group Method using Arithmetic averages) algorithm [14].
T5099	20995-21007	JJ	denotes	Phylogenetic
T5100	21008-21012	NN	denotes	tree
T5102	21013-21016	VBD	denotes	was
T5101	21017-21028	VBN	denotes	constructed
T5103	21029-21031	IN	denotes	by
T5104	21032-21040	NN	denotes	Clustalw
T5105	21041-21048	NN	denotes	program
T5106	21049-21050	-LRB-	denotes	(
T5107	21050-21057	NN	denotes	version
T5108	21058-21062	CD	denotes	1.81
T5109	21062-21063	-RRB-	denotes	)
T5110	21064-21069	VBG	denotes	using
T5111	21070-21075	NN	denotes	UPGMA
T5113	21076-21077	-LRB-	denotes	(
T5114	21077-21087	JJ	denotes	Unweighted
T5116	21088-21092	NN	denotes	Pair
T5117	21093-21098	NN	denotes	Group
T5115	21099-21105	NN	denotes	Method
T5118	21106-21111	VBG	denotes	using
T5119	21112-21122	NN	denotes	Arithmetic
T5120	21123-21131	NNS	denotes	averages
T5121	21131-21132	-RRB-	denotes	)
T5112	21133-21142	NN	denotes	algorithm
T5122	21143-21144	-LRB-	denotes	[
T5123	21144-21146	CD	denotes	14
T5124	21146-21147	-RRB-	denotes	]
T5125	21147-21148	.	denotes	.
T5264	21150-21158	JJ	denotes	Neuronal
T5266	21159-21163	NN	denotes	cell
T5265	21164-21175	NN	denotes	preparation
T5267	21176-21179	CC	denotes	and
T5268	21180-21195	NN	denotes	immunostanining
T5269	21195-21265	sentence	denotes	Hippocampal neuron cultures were prepared as previously reported [6].
T5270	21196-21207	JJ	denotes	Hippocampal
T5272	21208-21214	NN	denotes	neuron
T5271	21215-21223	NNS	denotes	cultures
T5274	21224-21228	VBD	denotes	were
T5273	21229-21237	VBN	denotes	prepared
T5275	21238-21240	IN	denotes	as
T5277	21241-21251	RB	denotes	previously
T5276	21252-21260	VBN	denotes	reported
T5278	21261-21262	-LRB-	denotes	[
T5279	21262-21263	CD	denotes	6
T5280	21263-21264	-RRB-	denotes	]
T5281	21264-21265	.	denotes	.
T5282	21265-21352	sentence	denotes	In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero.
T5283	21266-21268	IN	denotes	In
T5285	21269-21274	NN	denotes	brief
T5286	21274-21276	,	denotes	,
T5287	21276-21279	DT	denotes	the
T5288	21280-21293	NNS	denotes	hippocampuses
T5289	21294-21298	VBD	denotes	were
T5284	21299-21308	VBN	denotes	dissected
T5290	21309-21312	RP	denotes	out
T5291	21313-21317	IN	denotes	from
T5292	21318-21323	NN	denotes	mouse
T5293	21324-21331	NNS	denotes	embryos
T5294	21332-21334	IN	denotes	at
T5295	21335-21337	CD	denotes	16
T5296	21338-21342	NNS	denotes	days
T5297	21343-21345	FW	denotes	in
T5298	21346-21351	FW	denotes	utero
T5299	21351-21352	.	denotes	.
T5300	21352-21529	sentence	denotes	The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies).
T5301	21353-21356	DT	denotes	The
T5302	21357-21364	NNS	denotes	tissues
T5304	21365-21369	VBD	denotes	were
T5305	21370-21374	RB	denotes	then
T5303	21375-21384	VBN	denotes	incubated
T5306	21385-21388	IN	denotes	for
T5307	21389-21391	CD	denotes	20
T5308	21392-21395	NN	denotes	min
T5309	21396-21398	IN	denotes	at
T5310	21399-21401	CD	denotes	37
T5311	21401-21403	NN	denotes	°C
T5312	21404-21406	IN	denotes	in
T5313	21407-21410	NN	denotes	MEM
T5314	21411-21412	-LRB-	denotes	(
T5315	21412-21419	JJ	denotes	minimum
T5317	21420-21429	JJ	denotes	essential
T5316	21430-21436	NN	denotes	medium
T5318	21436-21437	-RRB-	denotes	)
T5319	21438-21446	VBN	denotes	modified
T5320	21447-21450	IN	denotes	for
T5321	21451-21461	NN	denotes	suspension
T5322	21462-21469	NN	denotes	culture
T5323	21470-21471	-LRB-	denotes	(
T5325	21471-21475	NNP	denotes	Life
T5324	21476-21488	NNP	denotes	Technologies
T5326	21488-21489	-RRB-	denotes	)
T5327	21490-21494	CC	denotes	plus
T5328	21495-21499	CD	denotes	0.25
T5329	21499-21500	NN	denotes	%
T5330	21501-21508	NN	denotes	trypsin
T5331	21509-21510	-LRB-	denotes	(
T5333	21510-21514	NNP	denotes	Life
T5332	21515-21527	NNP	denotes	Technologies
T5334	21527-21528	-RRB-	denotes	)
T5335	21528-21529	.	denotes	.
T5336	21529-21689	sentence	denotes	The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below.
T5337	21530-21533	DT	denotes	The
T5339	21534-21545	JJ	denotes	dissociated
T5340	21546-21557	JJ	denotes	hippocampal
T5338	21558-21565	NNS	denotes	neurons
T5342	21566-21570	VBD	denotes	were
T5341	21571-21577	VBN	denotes	plated
T5343	21578-21580	IN	denotes	on
T5344	21581-21586	NN	denotes	glass
T5345	21587-21597	NNS	denotes	coverslips
T5346	21598-21604	VBN	denotes	coated
T5347	21605-21609	IN	denotes	with
T5348	21610-21611	DT	denotes	a
T5350	21612-21621	JJ	denotes	confluent
T5349	21622-21631	NN	denotes	monolayer
T5351	21632-21634	IN	denotes	of
T5352	21635-21640	NN	denotes	mouse
T5354	21641-21649	JJ	denotes	cortical
T5353	21650-21660	NNS	denotes	astrocytes
T5355	21661-21669	VBN	denotes	obtained
T5356	21670-21672	IN	denotes	as
T5357	21673-21682	VBN	denotes	described
T5358	21683-21688	RB	denotes	below
T5359	21688-21689	.	denotes	.
T5360	21689-21765	sentence	denotes	The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2.
T5361	21690-21693	DT	denotes	The
T5362	21694-21701	NNS	denotes	neurons
T5364	21702-21706	VBD	denotes	were
T5363	21707-21717	VBN	denotes	maintained
T5365	21718-21720	IN	denotes	at
T5366	21721-21723	CD	denotes	37
T5367	21723-21725	NN	denotes	°C
T5368	21726-21728	IN	denotes	in
T5369	21729-21730	DT	denotes	a
T5371	21731-21741	JJ	denotes	humidified
T5370	21742-21752	NN	denotes	atmosphere
T5372	21753-21757	IN	denotes	with
T5373	21758-21759	CD	denotes	5
T5374	21759-21760	NN	denotes	%
T5375	21761-21764	NN	denotes	CO2
T5376	21764-21765	.	denotes	.
T5377	21765-21918	sentence	denotes	Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent.
T5378	21766-21774	JJ	denotes	Cortical
T5379	21775-21785	NNS	denotes	astrocytes
T5381	21786-21797	VBN	denotes	dissociated
T5382	21798-21802	IN	denotes	from
T5383	21803-21810	JJ	denotes	newborn
T5384	21811-21816	NN	denotes	mouse
T5385	21817-21825	NNS	denotes	cortices
T5386	21826-21830	VBD	denotes	were
T5380	21831-21836	VBN	denotes	grown
T5387	21837-21839	IN	denotes	in
T5388	21840-21847	NN	denotes	culture
T5389	21848-21854	NNS	denotes	flasks
T5390	21855-21857	IN	denotes	at
T5391	21858-21860	CD	denotes	37
T5392	21860-21862	NN	denotes	°C
T5393	21863-21865	IN	denotes	in
T5394	21866-21867	DT	denotes	a
T5396	21868-21878	JJ	denotes	humidified
T5395	21879-21889	NN	denotes	atmosphere
T5397	21890-21894	IN	denotes	with
T5398	21895-21896	CD	denotes	5
T5399	21896-21897	NN	denotes	%
T5400	21898-21901	NN	denotes	CO2
T5401	21902-21907	IN	denotes	until
T5402	21908-21917	JJ	denotes	confluent
T5403	21917-21918	.	denotes	.
T5404	21918-22027	sentence	denotes	The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C.
T5405	21919-21922	DT	denotes	The
T5406	21923-21928	NNS	denotes	cells
T5408	21929-21933	VBD	denotes	were
T5407	21934-21941	VBN	denotes	exposed
T5409	21942-21944	IN	denotes	to
T5410	21945-21947	CD	denotes	10
T5412	21947-21948	SYM	denotes	-
T5411	21948-21949	CD	denotes	5
T5413	21950-21951	NN	denotes	M
T5415	21952-21960	NN	denotes	cytosine
T5414	21961-21972	NN	denotes	arabinoside
T5416	21973-21974	-LRB-	denotes	(
T5417	21974-21979	NNP	denotes	Sigma
T5418	21979-21980	-RRB-	denotes	)
T5419	21981-21984	CC	denotes	and
T5420	21985-21993	VBN	denotes	cultured
T5421	21994-21997	IN	denotes	for
T5422	21998-22008	JJ	denotes	additional
T5424	22009-22011	CD	denotes	12
T5426	22011-22012	SYM	denotes	–
T5425	22012-22014	CD	denotes	24
T5423	22015-22018	NNS	denotes	hrs
T5427	22019-22021	IN	denotes	at
T5428	22022-22024	CD	denotes	37
T5429	22024-22026	NN	denotes	°C
T5430	22026-22027	.	denotes	.
T5431	22027-22119	sentence	denotes	After remove of the media with cellular debris, the cells were used for coating coverslips.
T5432	22028-22033	IN	denotes	After
T5434	22034-22040	NN	denotes	remove
T5435	22041-22043	IN	denotes	of
T5436	22044-22047	DT	denotes	the
T5437	22048-22053	NNS	denotes	media
T5438	22054-22058	IN	denotes	with
T5439	22059-22067	JJ	denotes	cellular
T5440	22068-22074	NN	denotes	debris
T5441	22074-22076	,	denotes	,
T5442	22076-22079	DT	denotes	the
T5443	22080-22085	NNS	denotes	cells
T5444	22086-22090	VBD	denotes	were
T5433	22091-22095	VBN	denotes	used
T5445	22096-22099	IN	denotes	for
T5446	22100-22107	VBG	denotes	coating
T5447	22108-22118	NNS	denotes	coverslips
T5448	22118-22119	.	denotes	.
T5449	22119-22341	sentence	denotes	For immunostaining, neuronal cells on the coverslips were first fixed in PBS containing 4% paraformaldehyde (PFA) for 12 hrs at 4°C and then incubated in a solution containing 4% PFA and 0.4% Triton X-100 at 4°C for 1 hr.
T5450	22120-22123	IN	denotes	For
T5452	22124-22138	NN	denotes	immunostaining
T5453	22138-22140	,	denotes	,
T5454	22140-22148	JJ	denotes	neuronal
T5455	22149-22154	NNS	denotes	cells
T5456	22155-22157	IN	denotes	on
T5457	22158-22161	DT	denotes	the
T5458	22162-22172	NNS	denotes	coverslips
T5459	22173-22177	VBD	denotes	were
T5460	22178-22183	RB	denotes	first
T5451	22184-22189	VBN	denotes	fixed
T5461	22190-22192	IN	denotes	in
T5462	22193-22196	NN	denotes	PBS
T5463	22197-22207	VBG	denotes	containing
T5464	22208-22209	CD	denotes	4
T5465	22209-22210	NN	denotes	%
T5466	22211-22227	NN	denotes	paraformaldehyde
T5467	22228-22229	-LRB-	denotes	(
T5468	22229-22232	NN	denotes	PFA
T5469	22232-22233	-RRB-	denotes	)
T5470	22234-22237	IN	denotes	for
T5471	22238-22240	CD	denotes	12
T5472	22241-22244	NNS	denotes	hrs
T5473	22245-22247	IN	denotes	at
T5474	22248-22249	CD	denotes	4
T5475	22249-22251	NN	denotes	°C
T5476	22252-22255	CC	denotes	and
T5477	22256-22260	RB	denotes	then
T5478	22261-22270	VBN	denotes	incubated
T5479	22271-22273	IN	denotes	in
T5480	22274-22275	DT	denotes	a
T5481	22276-22284	NN	denotes	solution
T5482	22285-22295	VBG	denotes	containing
T5483	22296-22297	CD	denotes	4
T5484	22297-22298	NN	denotes	%
T5485	22299-22302	NN	denotes	PFA
T5486	22303-22306	CC	denotes	and
T5487	22307-22310	CD	denotes	0.4
T5488	22310-22311	NN	denotes	%
T5490	22312-22318	NN	denotes	Triton
T5491	22319-22320	NN	denotes	X
T5492	22320-22321	HYPH	denotes	-
T5489	22321-22324	NN	denotes	100
T5493	22325-22327	IN	denotes	at
T5494	22328-22329	CD	denotes	4
T5495	22329-22331	NN	denotes	°C
T5496	22332-22335	IN	denotes	for
T5497	22336-22337	CD	denotes	1
T5498	22338-22340	NN	denotes	hr
T5499	22340-22341	.	denotes	.
T5500	22341-22560	sentence	denotes	After washing with PBS three times, the cells were incubated with a blocking solution containing 1:30 normal goat serum, and subsequently incubated with a rabbit polyclonal anti-ACDP antibody (1:3000) overnight at 4°C.
T5501	22342-22347	IN	denotes	After
T5503	22348-22355	VBG	denotes	washing
T5504	22356-22360	IN	denotes	with
T5505	22361-22364	NN	denotes	PBS
T5506	22365-22370	CD	denotes	three
T5507	22371-22376	NNS	denotes	times
T5508	22376-22378	,	denotes	,
T5509	22378-22381	DT	denotes	the
T5510	22382-22387	NNS	denotes	cells
T5511	22388-22392	VBD	denotes	were
T5502	22393-22402	VBN	denotes	incubated
T5512	22403-22407	IN	denotes	with
T5513	22408-22409	DT	denotes	a
T5515	22410-22418	VBG	denotes	blocking
T5514	22419-22427	NN	denotes	solution
T5516	22428-22438	VBG	denotes	containing
T5517	22439-22440	CD	denotes	1
T5519	22440-22441	SYM	denotes	:
T5520	22441-22443	CD	denotes	30
T5521	22444-22450	JJ	denotes	normal
T5522	22451-22455	NN	denotes	goat
T5518	22456-22461	NN	denotes	serum
T5523	22461-22463	,	denotes	,
T5524	22463-22466	CC	denotes	and
T5525	22467-22479	RB	denotes	subsequently
T5526	22480-22489	VBN	denotes	incubated
T5527	22490-22494	IN	denotes	with
T5528	22495-22496	DT	denotes	a
T5530	22497-22503	NN	denotes	rabbit
T5531	22504-22514	JJ	denotes	polyclonal
T5532	22515-22524	JJ	denotes	anti-ACDP
T5529	22525-22533	NN	denotes	antibody
T5533	22534-22535	-LRB-	denotes	(
T5534	22535-22536	CD	denotes	1
T5535	22536-22537	SYM	denotes	:
T5536	22537-22541	CD	denotes	3000
T5537	22541-22542	-RRB-	denotes	)
T5538	22543-22552	RB	denotes	overnight
T5539	22553-22555	IN	denotes	at
T5540	22556-22557	CD	denotes	4
T5541	22557-22559	NN	denotes	°C
T5542	22559-22560	.	denotes	.
T5543	22560-22776	sentence	denotes	After extensive washing with 1% goat serum PBS solution, the cells were incubated with an Alex 488 conjugated secondary antibody (1:100 in 1% goat serum PBS solution, Molecular Probes) for 3 hrs at room temperature.
T5544	22561-22566	IN	denotes	After
T5546	22567-22576	JJ	denotes	extensive
T5547	22577-22584	NN	denotes	washing
T5548	22585-22589	IN	denotes	with
T5549	22590-22591	CD	denotes	1
T5550	22591-22592	NN	denotes	%
T5552	22593-22597	NN	denotes	goat
T5551	22598-22603	NN	denotes	serum
T5554	22604-22607	NN	denotes	PBS
T5553	22608-22616	NN	denotes	solution
T5555	22616-22618	,	denotes	,
T5556	22618-22621	DT	denotes	the
T5557	22622-22627	NNS	denotes	cells
T5558	22628-22632	VBD	denotes	were
T5545	22633-22642	VBN	denotes	incubated
T5559	22643-22647	IN	denotes	with
T5560	22648-22650	DT	denotes	an
T5562	22651-22655	NNP	denotes	Alex
T5563	22656-22659	CD	denotes	488
T5564	22660-22670	JJ	denotes	conjugated
T5565	22671-22680	JJ	denotes	secondary
T5561	22681-22689	NN	denotes	antibody
T5566	22690-22691	-LRB-	denotes	(
T5568	22691-22692	CD	denotes	1
T5569	22692-22693	SYM	denotes	:
T5570	22693-22696	CD	denotes	100
T5571	22697-22699	IN	denotes	in
T5572	22700-22701	CD	denotes	1
T5573	22701-22702	NN	denotes	%
T5575	22703-22707	NN	denotes	goat
T5574	22708-22713	NN	denotes	serum
T5577	22714-22717	NN	denotes	PBS
T5576	22718-22726	NN	denotes	solution
T5578	22726-22728	,	denotes	,
T5579	22728-22737	NNP	denotes	Molecular
T5567	22738-22744	NNP	denotes	Probes
T5580	22744-22745	-RRB-	denotes	)
T5581	22746-22749	IN	denotes	for
T5582	22750-22751	CD	denotes	3
T5583	22752-22755	NNS	denotes	hrs
T5584	22756-22758	IN	denotes	at
T5585	22759-22763	NN	denotes	room
T5586	22764-22775	NN	denotes	temperature
T5587	22775-22776	.	denotes	.
T5588	22776-22935	sentence	denotes	Following final washes with 1% goat serum PBS solution, the neuronal cells on the coverslips were cover-slipped with a glycerol-based anti-photobleach medium.
T5589	22777-22786	IN	denotes	Following
T5591	22787-22792	JJ	denotes	final
T5592	22793-22799	NNS	denotes	washes
T5593	22800-22804	IN	denotes	with
T5594	22805-22806	CD	denotes	1
T5595	22806-22807	NN	denotes	%
T5597	22808-22812	NN	denotes	goat
T5596	22813-22818	NN	denotes	serum
T5599	22819-22822	NN	denotes	PBS
T5598	22823-22831	NN	denotes	solution
T5600	22831-22833	,	denotes	,
T5601	22833-22836	DT	denotes	the
T5603	22837-22845	JJ	denotes	neuronal
T5602	22846-22851	NNS	denotes	cells
T5604	22852-22854	IN	denotes	on
T5605	22855-22858	DT	denotes	the
T5606	22859-22869	NNS	denotes	coverslips
T5607	22870-22874	VBD	denotes	were
T5608	22875-22880	NN	denotes	cover
T5609	22880-22881	HYPH	denotes	-
T5590	22881-22888	VBN	denotes	slipped
T5610	22889-22893	IN	denotes	with
T5611	22894-22895	DT	denotes	a
T5613	22896-22904	NN	denotes	glycerol
T5615	22904-22905	HYPH	denotes	-
T5614	22905-22910	VBN	denotes	based
T5616	22911-22927	JJ	denotes	anti-photobleach
T5612	22928-22934	NN	denotes	medium
T5617	22934-22935	.	denotes	.
T5618	22935-22999	sentence	denotes	The cells were viewed under a confocal microscope (Carl Zeiss).
T5619	22936-22939	DT	denotes	The
T5620	22940-22945	NNS	denotes	cells
T5622	22946-22950	VBD	denotes	were
T5621	22951-22957	VBN	denotes	viewed
T5623	22958-22963	IN	denotes	under
T5624	22964-22965	DT	denotes	a
T5626	22966-22974	JJ	denotes	confocal
T5625	22975-22985	NN	denotes	microscope
T5627	22986-22987	-LRB-	denotes	(
T5629	22987-22991	NNP	denotes	Carl
T5628	22992-22997	NNP	denotes	Zeiss
T5630	22997-22998	-RRB-	denotes	)
T5631	22998-22999	.	denotes	.
T5632	22999-23115	sentence	denotes	Images were captured with a CCD camera and acquired by the Scion Image software (Scion Corporation, Frederick, MD).
T5633	23000-23006	NNS	denotes	Images
T5635	23007-23011	VBD	denotes	were
T5634	23012-23020	VBN	denotes	captured
T5636	23021-23025	IN	denotes	with
T5637	23026-23027	DT	denotes	a
T5639	23028-23031	NN	denotes	CCD
T5638	23032-23038	NN	denotes	camera
T5640	23039-23042	CC	denotes	and
T5641	23043-23051	VBN	denotes	acquired
T5642	23052-23054	IN	denotes	by
T5643	23055-23058	DT	denotes	the
T5645	23059-23064	NNP	denotes	Scion
T5646	23065-23070	NN	denotes	Image
T5644	23071-23079	NN	denotes	software
T5647	23080-23081	-LRB-	denotes	(
T5649	23081-23086	NNP	denotes	Scion
T5648	23087-23098	NNP	denotes	Corporation
T5650	23098-23100	,	denotes	,
T5651	23100-23109	NNP	denotes	Frederick
T5652	23109-23111	,	denotes	,
T5653	23111-23113	NNP	denotes	MD
T5654	23113-23114	-RRB-	denotes	)
T5655	23114-23115	.	denotes	.
T5677	23117-23121	NN	denotes	Gene
T5678	23122-23126	NN	denotes	bank
T5680	23127-23136	NN	denotes	accession
T5679	23137-23143	NN	denotes	number
T5681	23143-23326	sentence	denotes	The cDNA sequences for the Acdp gene family have already been deposited in gene bank with accession numbers AF202994 (Acdp1), AF216961 (Acdp2), AF216964 (Acdp3) and AF216963 (Acdp4).
T5682	23144-23147	DT	denotes	The
T5684	23148-23152	NN	denotes	cDNA
T5683	23153-23162	NNS	denotes	sequences
T5686	23163-23166	IN	denotes	for
T5687	23167-23170	DT	denotes	the
T5689	23171-23175	NN	denotes	Acdp
T5690	23176-23180	NN	denotes	gene
T5688	23181-23187	NN	denotes	family
T5691	23188-23192	VBP	denotes	have
T5692	23193-23200	RB	denotes	already
T5693	23201-23205	VBN	denotes	been
T5685	23206-23215	VBN	denotes	deposited
T5694	23216-23218	IN	denotes	in
T5695	23219-23223	NN	denotes	gene
T5696	23224-23228	NN	denotes	bank
T5697	23229-23233	IN	denotes	with
T5698	23234-23243	NN	denotes	accession
T5700	23244-23251	NNS	denotes	numbers
T5699	23252-23260	NN	denotes	AF202994
T5701	23261-23262	-LRB-	denotes	(
T5702	23262-23267	NN	denotes	Acdp1
T5703	23267-23268	-RRB-	denotes	)
T5704	23268-23270	,	denotes	,
T5705	23270-23278	NN	denotes	AF216961
T5706	23279-23280	-LRB-	denotes	(
T5707	23280-23285	NN	denotes	Acdp2
T5708	23285-23286	-RRB-	denotes	)
T5709	23286-23288	,	denotes	,
T5710	23288-23296	NN	denotes	AF216964
T5711	23297-23298	-LRB-	denotes	(
T5712	23298-23303	NN	denotes	Acdp3
T5713	23303-23304	-RRB-	denotes	)
T5714	23305-23308	CC	denotes	and
T5715	23309-23317	NN	denotes	AF216963
T5716	23318-23319	-LRB-	denotes	(
T5717	23319-23324	NN	denotes	Acdp4
T5718	23324-23325	-RRB-	denotes	)
T5719	23325-23326	.	denotes	.
R10	T226	T223	compound	gene,family
R100	T327	T314	punct	", ",encode
R1000	T2035	T2036	nmod	nucleotide,sequences
R1001	T2036	T2033	pobj	sequences,of
R1002	T2037	T2035	cc	and,nucleotide
R1003	T2038	T2035	conj	AA,nucleotide
R1004	T2039	T2032	prep	to,homologies
R1005	T2040	T2041	det	the,genes
R1006	T2041	T2039	pobj	genes,to
R1007	T2042	T2041	amod	human,genes
R1008	T2043	T2041	compound	ACDP,genes
R1009	T2044	T2045	punct	(,Table
R101	T328	T314	advmod	respectively,encode
R1010	T2045	T2029	parataxis	Table,showed
R1011	T2046	T2045	nummod	1,Table
R1012	T2047	T2045	punct	),Table
R1013	T2048	T2029	punct	.,showed
R1014	T2050	T2051	det	The,homologies
R1015	T2051	T2053	nsubjpass	homologies,observed
R1016	T2052	T2051	amod	highest,homologies
R1017	T2054	T2053	auxpass	were,observed
R1018	T2055	T2053	prep	between,observed
R1019	T2056	T2057	det	the,ACDP2
R102	T329	T288	punct	.,contain
R1020	T2057	T2055	pobj	ACDP2,between
R1021	T2058	T2057	amod	human,ACDP2
R1022	T2059	T2057	cc	and,ACDP2
R1023	T2060	T2061	det	the,Acdp2
R1024	T2061	T2063	compound	Acdp2,gene
R1025	T2062	T2061	compound	mouse,Acdp2
R1026	T2063	T2057	conj	gene,ACDP2
R1027	T2064	T2065	punct	(,%
R1028	T2065	T2053	parataxis	%,observed
R1029	T2066	T2065	nummod	91,%
R103	T331	T332	det	The,genes
R1030	T2067	T2065	prep	of,%
R1031	T2068	T2069	compound	nucleotide,identity
R1032	T2069	T2067	pobj	identity,of
R1033	T2070	T2065	punct	", ",%
R1034	T2071	T2072	nummod	97,%
R1035	T2072	T2065	conj	%,%
R1036	T2073	T2072	prep	of,%
R1037	T2074	T2075	compound	AA,identity
R1038	T2075	T2073	pobj	identity,of
R1039	T2076	T2072	cc	and,%
R104	T332	T335	nsubj	genes,showed
R1040	T2077	T2078	nummod	99.4,%
R1041	T2078	T2072	conj	%,%
R1042	T2079	T2078	prep	of,%
R1043	T2080	T2081	compound	AA,homology
R1044	T2081	T2079	pobj	homology,of
R1045	T2082	T2065	punct	),%
R1046	T2083	T2053	punct	.,observed
R1047	T2085	T2086	prep	In,showed
R1048	T2086	T2105	ccomp	showed,showed
R1049	T2087	T2085	pobj	addition,In
R105	T333	T332	compound	mouse,genes
R1050	T2088	T2086	punct	", ",showed
R1051	T2089	T2090	det	the,sequences
R1052	T2090	T2086	nsubj	sequences,showed
R1053	T2091	T2092	nummod	5,UTR
R1054	T2092	T2090	compound	UTR,sequences
R1055	T2093	T2091	punct	',5
R1056	T2094	T2090	compound	nucleotide,sequences
R1057	T2095	T2096	punct	(,bp
R1058	T2096	T2090	parataxis	bp,sequences
R1059	T2097	T2096	nummod	20,bp
R106	T334	T332	compound	Acdp,genes
R1060	T2098	T2096	prep	of,bp
R1061	T2099	T2098	pobj	nucleotides,of
R1062	T2100	T2096	prep	before,bp
R1063	T2101	T2102	compound	start,codon
R1064	T2102	T2100	pobj	codon,before
R1065	T2103	T2096	punct	),bp
R1066	T2104	T2086	advmod	also,showed
R1067	T2106	T2107	amod	high,homologies
R1068	T2107	T2086	dobj	homologies,showed
R1069	T2108	T2107	prep	to,homologies
R107	T336	T337	advmod	very,strong
R1070	T2109	T2110	det	the,homologs
R1071	T2110	T2108	pobj	homologs,to
R1072	T2111	T2110	amod	human,homologs
R1073	T2112	T2105	punct	", ",showed
R1074	T2113	T2105	prep	for,showed
R1075	T2114	T2113	pobj	example,for
R1076	T2115	T2105	punct	", ",showed
R1077	T2116	T2117	det	the,sequence
R1078	T2117	T2105	nsubj	sequence,showed
R1079	T2118	T2117	nmod	Acdp2,sequence
R108	T337	T338	amod	strong,homologies
R1080	T2119	T2120	nummod	5,UTR
R1081	T2120	T2117	compound	UTR,sequence
R1082	T2121	T2119	punct	',5
R1083	T2122	T2123	nummod	95,%
R1084	T2123	T2124	compound	%,identities
R1085	T2124	T2105	dobj	identities,showed
R1086	T2125	T2105	prep	to,showed
R1087	T2126	T2127	poss	its,homolog
R1088	T2127	T2125	pobj	homolog,to
R1089	T2128	T2127	amod	human,homolog
R109	T338	T335	dobj	homologies,showed
R1090	T2129	T2105	punct	.,showed
R1091	T2131	T2132	advmod	However,were
R1092	T2133	T2132	punct	", ",were
R1093	T2134	T2135	det	the,homologies
R1094	T2135	T2132	nsubj	homologies,were
R1095	T2136	T2135	prep	in,homologies
R1096	T2137	T2138	det	the,sequences
R1097	T2138	T2136	pobj	sequences,in
R1098	T2139	T2140	nummod	3,UTR
R1099	T2140	T2138	compound	UTR,sequences
R11	T231	T232	nsubj	We,cloned
R110	T339	T340	punct	(,%
R1100	T2141	T2139	punct	',3
R1101	T2142	T2143	punct	(,bp
R1102	T2143	T2135	parataxis	bp,homologies
R1103	T2144	T2143	nummod	20,bp
R1104	T2145	T2143	prep	of,bp
R1105	T2146	T2145	pobj	nucleotides,of
R1106	T2147	T2143	prep	after,bp
R1107	T2148	T2149	compound	stop,codon
R1108	T2149	T2147	pobj	codon,after
R1109	T2150	T2143	punct	),bp
R111	T340	T338	parataxis	%,homologies
R1110	T2151	T2152	advmod	much,lower
R1111	T2152	T2132	acomp	lower,were
R1112	T2153	T2154	punct	(,%
R1113	T2154	T2132	parataxis	%,were
R1114	T2155	T2156	quantmod	40,55
R1115	T2156	T2154	nummod	55,%
R1116	T2157	T2156	punct	–,55
R1117	T2158	T2154	punct	),%
R1118	T2159	T2132	prep	for,were
R1119	T2160	T2161	det	all,genes
R112	T341	T342	punct	>,90
R1120	T2161	T2159	pobj	genes,for
R1121	T2162	T2161	compound	Acdp,genes
R1122	T2163	T2161	prep	except,genes
R1123	T2164	T2163	pobj	Acdp4,except
R1124	T2165	T2166	punct	(,identity
R1125	T2166	T2164	parataxis	identity,Acdp4
R1126	T2167	T2166	nummod	90,identity
R1127	T2168	T2167	quantmod	%,90
R1128	T2169	T2166	prep	to,identity
R1129	T2170	T2171	poss	its,homolog
R113	T342	T340	nummod	90,%
R1130	T2171	T2169	pobj	homolog,to
R1131	T2172	T2171	amod	human,homolog
R1132	T2173	T2166	punct	),identity
R1133	T2174	T2132	punct	.,were
R1134	T2176	T2177	det	The,domain
R1135	T2177	T2180	nsubj	domain,has
R1136	T2178	T2177	amod	ancient,domain
R1137	T2179	T2177	amod	conserved,domain
R1138	T2181	T2177	punct	(,domain
R1139	T2182	T2177	appos	ACD,domain
R114	T343	T340	punct	),%
R1140	T2183	T2180	punct	),has
R1141	T2184	T2185	nummod	55.3,%
R1142	T2185	T2180	dobj	%,has
R1143	T2186	T2185	prep	of,%
R1144	T2187	T2188	compound	AA,identity
R1145	T2188	T2186	pobj	identity,of
R1146	T2189	T2185	cc	and,%
R1147	T2190	T2191	nummod	83.3,%
R1148	T2191	T2185	conj	%,%
R1149	T2192	T2191	prep	of,%
R115	T344	T338	prep	in,homologies
R1150	T2193	T2192	pobj	homology,of
R1151	T2194	T2180	prep	between,has
R1152	T2195	T2196	det	all,proteins
R1153	T2196	T2194	pobj	proteins,between
R1154	T2197	T2196	nmod	mouse,proteins
R1155	T2198	T2197	cc	and,mouse
R1156	T2199	T2197	conj	human,mouse
R1157	T2200	T2196	compound	ACDP,proteins
R1158	T2201	T2202	punct	(,Fig.
R1159	T2202	T2180	parataxis	Fig.,has
R116	T345	T346	preconj	both,nucleotide
R1160	T2203	T2202	nummod	2,Fig.
R1161	T2204	T2202	punct	),Fig.
R1162	T2205	T2180	punct	.,has
R1163	T2207	T2208	det	The,domain
R1164	T2208	T2210	nsubjpass	domain,conserved
R1165	T2209	T2208	compound	ACD,domain
R1166	T2211	T2210	auxpass	is,conserved
R1167	T2212	T2210	advmod	evolutionarily,conserved
R1168	T2213	T2210	prep	in,conserved
R1169	T2214	T2215	amod	divergent,species
R117	T346	T347	nmod	nucleotide,sequences
R1170	T2215	T2213	pobj	species,in
R1171	T2216	T2215	acl	ranging,species
R1172	T2217	T2216	prep	from,ranging
R1173	T2218	T2217	pobj	bacteria,from
R1174	T2219	T2218	punct	", ",bacteria
R1175	T2220	T2218	appos	yeast,bacteria
R1176	T2221	T2218	punct	", ",bacteria
R1177	T2222	T2223	compound	C.,elegans
R1178	T2223	T2218	appos	elegans,bacteria
R1179	T2224	T2218	punct	", ",bacteria
R118	T347	T344	pobj	sequences,in
R1180	T2225	T2226	compound	D.,melanogaster
R1181	T2226	T2218	appos	melanogaster,bacteria
R1182	T2227	T2218	punct	", ",bacteria
R1183	T2228	T2218	appos	mouse,bacteria
R1184	T2229	T2217	prep	to,from
R1185	T2230	T2229	pobj	human,to
R1186	T2231	T2232	punct	(,Fig.
R1187	T2232	T2210	parataxis	Fig.,conserved
R1188	T2233	T2232	nummod	3,Fig.
R1189	T2234	T2232	punct	),Fig.
R119	T348	T346	cc	and,nucleotide
R1190	T2235	T2210	punct	.,conserved
R1191	T2237	T2238	advmod	Particularly,showed
R1192	T2239	T2238	punct	", ",showed
R1193	T2240	T2241	mark	as,shown
R1194	T2241	T2238	advcl	shown,showed
R1195	T2242	T2241	prep	in,shown
R1196	T2243	T2242	pobj	Fig.,in
R1197	T2244	T2243	nummod	3,Fig.
R1198	T2245	T2238	punct	", ",showed
R1199	T2246	T2247	compound	Acdp,proteins
R12	T233	T232	aux	have,cloned
R120	T349	T350	compound	amino,acid
R1200	T2247	T2238	nsubj	proteins,showed
R1201	T2248	T2249	advmod	very,strong
R1202	T2249	T2250	amod	strong,homology
R1203	T2250	T2238	dobj	homology,showed
R1204	T2251	T2250	compound	AA,homology
R1205	T2252	T2250	prep	to,homology
R1206	T2253	T2254	compound	bacteria,protein
R1207	T2254	T2252	pobj	protein,to
R1208	T2255	T2254	compound	CorC,protein
R1209	T2256	T2257	punct	(,identity
R121	T350	T346	conj	acid,nucleotide
R1210	T2257	T2254	parataxis	identity,protein
R1211	T2258	T2259	nummod	35,%
R1212	T2259	T2257	compound	%,identity
R1213	T2260	T2257	compound	AA,identity
R1214	T2261	T2257	prep	with,identity
R1215	T2262	T2263	nummod	55,%
R1216	T2263	T2264	compound	%,homology
R1217	T2264	T2261	pobj	homology,with
R1218	T2265	T2257	punct	),identity
R1219	T2266	T2254	punct	", ",protein
R122	T351	T338	prep	to,homologies
R1220	T2267	T2268	dep	which,involved
R1221	T2268	T2254	relcl	involved,protein
R1222	T2269	T2268	auxpass	is,involved
R1223	T2270	T2268	prep	in,involved
R1224	T2271	T2272	nmod	magnesium,efflux
R1225	T2272	T2270	pobj	efflux,in
R1226	T2273	T2271	cc	and,magnesium
R1227	T2274	T2271	conj	cobalt,magnesium
R1228	T2275	T2276	punct	[,7
R1229	T2276	T2238	parataxis	7,showed
R123	T352	T353	poss	their,counterparts
R1230	T2277	T2276	punct	],7
R1231	T2278	T2238	punct	.,showed
R1232	T2280	T2281	amod	High,homology
R1233	T2281	T2283	nsubjpass	homology,observed
R1234	T2282	T2281	compound	AA,homology
R1235	T2284	T2283	auxpass	was,observed
R1236	T2285	T2283	advmod	also,observed
R1237	T2286	T2283	prep	between,observed
R1238	T2287	T2288	det	the,proteins
R1239	T2288	T2286	pobj	proteins,between
R124	T353	T351	pobj	counterparts,to
R1240	T2289	T2288	compound	Acdp,proteins
R1241	T2290	T2288	cc	and,proteins
R1242	T2291	T2292	det	the,protein
R1243	T2292	T2288	conj	protein,proteins
R1244	T2293	T2292	compound	yeast,protein
R1245	T2294	T2292	compound	Amip3,protein
R1246	T2295	T2296	punct	(,identity
R1247	T2296	T2283	parataxis	identity,observed
R1248	T2297	T2296	nummod	35,identity
R1249	T2298	T2297	quantmod	%,35
R125	T354	T353	amod	human,counterparts
R1250	T2299	T2296	compound	AA,identity
R1251	T2300	T2296	prep	with,identity
R1252	T2301	T2302	nummod	56,homology
R1253	T2302	T2300	pobj	homology,with
R1254	T2303	T2301	quantmod	%,56
R1255	T2304	T2296	punct	),identity
R1256	T2305	T2283	punct	.,observed
R1257	T2307	T2308	det	The,Amip3
R1258	T2308	T2309	nsubj	Amip3,is
R1259	T2310	T2309	acomp	likely,is
R126	T355	T335	punct	.,showed
R1260	T2311	T2312	aux	to,be
R1261	T2312	T2310	xcomp	be,likely
R1262	T2313	T2314	det	a,homologous
R1263	T2314	T2312	attr	homologous,be
R1264	T2315	T2314	prep	to,homologous
R1265	T2316	T2317	det	the,protein
R1266	T2317	T2315	pobj	protein,to
R1267	T2318	T2317	compound	bacteria,protein
R1268	T2319	T2317	compound	CorC,protein
R1269	T2320	T2309	punct	.,is
R127	T357	T358	prep	In,conserved
R1270	T2322	T2323	det	The,mutants
R1271	T2323	T2325	nsubj	mutants,confer
R1272	T2324	T2323	compound	Amip3,mutants
R1273	T2326	T2325	dobj	resistance,confer
R1274	T2327	T2326	prep	to,resistance
R1275	T2328	T2329	compound	copper,toxicity
R1276	T2329	T2327	pobj	toxicity,to
R1277	T2330	T2331	punct	(,Dr.
R1278	T2331	T2325	meta	Dr.,confer
R1279	T2332	T2331	amod	Personal,Dr.
R128	T359	T357	pobj	addition,In
R1280	T2333	T2331	nmod	communication,Dr.
R1281	T2334	T2331	nmod	with,Dr.
R1282	T2335	T2331	nmod	V.C.,Dr.
R1283	T2336	T2331	nmod	Culotte,Dr.
R1284	T2337	T2331	punct	", ",Dr.
R1285	T2338	T2331	nmod	John,Dr.
R1286	T2339	T2331	nmod	Hopkins,Dr.
R1287	T2340	T2331	nmod	Bloomberg,Dr.
R1288	T2341	T2331	punct	", ",Dr.
R1289	T2342	T2331	nmod	School,Dr.
R129	T360	T358	punct	", ",conserved
R1290	T2343	T2331	nmod	of,Dr.
R1291	T2344	T2331	nmod	Public,Dr.
R1292	T2345	T2331	nmod	Health,Dr.
R1293	T2346	T2331	punct	),Dr.
R1294	T2347	T2325	punct	.,confer
R1295	T2349	T2350	det	The,relationships
R1296	T2350	T2352	nsubjpass	relationships,illustrated
R1297	T2351	T2350	amod	evolutionary,relationships
R1298	T2353	T2350	prep	among,relationships
R1299	T2354	T2355	det	those,proteins
R13	T234	T232	advmod	recently,cloned
R130	T361	T362	preconj	both,nucleotide
R1300	T2355	T2353	pobj	proteins,among
R1301	T2356	T2352	auxpass	are,illustrated
R1302	T2357	T2352	prep	by,illustrated
R1303	T2358	T2359	det	a,tree
R1304	T2359	T2357	pobj	tree,by
R1305	T2360	T2359	amod	phylogenetic,tree
R1306	T2361	T2359	acl	constructed,tree
R1307	T2362	T2361	prep	based,constructed
R1308	T2363	T2362	prep	on,based
R1309	T2364	T2365	det	the,homology
R131	T362	T363	nmod	nucleotide,sequences
R1310	T2365	T2363	pobj	homology,on
R1311	T2366	T2365	compound	AA,homology
R1312	T2367	T2365	prep	of,homology
R1313	T2368	T2367	pobj	proteins,of
R1314	T2369	T2370	punct	(,Fig.
R1315	T2370	T2352	parataxis	Fig.,illustrated
R1316	T2371	T2370	nummod	4,Fig.
R1317	T2372	T2370	punct	),Fig.
R1318	T2373	T2352	punct	.,illustrated
R1319	T2376	T2377	nsubj	We,found
R132	T363	T358	nsubjpass	sequences,conserved
R1320	T2378	T2379	mark	that,contain
R1321	T2379	T2377	ccomp	contain,found
R1322	T2380	T2381	det	all,members
R1323	T2381	T2379	nsubj	members,contain
R1324	T2382	T2381	compound	mouse,members
R1325	T2383	T2381	compound	Acdp,members
R1326	T2384	T2385	nummod	four,domains
R1327	T2385	T2379	dobj	domains,contain
R1328	T2386	T2385	amod	distinct,domains
R1329	T2387	T2385	compound	transmembrane,domains
R133	T364	T362	cc	and,nucleotide
R1330	T2388	T2389	punct	(,Fig.
R1331	T2389	T2385	parataxis	Fig.,domains
R1332	T2390	T2389	nummod	5,Fig.
R1333	T2391	T2389	punct	),Fig.
R1334	T2392	T2385	punct	", ",domains
R1335	T2393	T2394	nummod	two,domains
R1336	T2394	T2385	conj	domains,domains
R1337	T2395	T2394	compound	CBS,domains
R1338	T2396	T2394	cc	and,domains
R1339	T2397	T2398	det	a,domain
R134	T365	T366	compound	amino,acid
R1340	T2398	T2394	conj	domain,domains
R1341	T2399	T2398	compound	DUF21,domain
R1342	T2400	T2401	dep	that,found
R1343	T2401	T2385	relcl	found,domains
R1344	T2402	T2401	auxpass	are,found
R1345	T2403	T2401	prep	in,found
R1346	T2404	T2405	nmod	bacteria,CorC
R1347	T2405	T2406	nmod	CorC,proteins
R1348	T2406	T2403	pobj	proteins,in
R1349	T2407	T2405	cc	and,CorC
R135	T366	T362	conj	acid,nucleotide
R1350	T2408	T2409	compound	yeast,Amip3
R1351	T2409	T2405	conj	Amip3,CorC
R1352	T2410	T2377	punct	.,found
R1353	T2412	T2413	compound	CBS,domains
R1354	T2413	T2414	nsubj	domains,are
R1355	T2415	T2416	amod	small,modules
R1356	T2416	T2414	attr	modules,are
R1357	T2417	T2416	amod	intracellular,modules
R1358	T2418	T2419	dep	that,found
R1359	T2419	T2416	relcl	found,modules
R136	T367	T363	prep	within,sequences
R1360	T2420	T2419	auxpass	are,found
R1361	T2421	T2419	advmod	mostly,found
R1362	T2422	T2419	prep	in,found
R1363	T2423	T2424	nummod	2,copies
R1364	T2424	T2422	pobj	copies,in
R1365	T2425	T2423	cc	or,2
R1366	T2426	T2423	conj	four,2
R1367	T2427	T2419	prep	within,found
R1368	T2428	T2429	det	a,protein
R1369	T2429	T2427	pobj	protein,within
R137	T368	T369	det	the,Domain
R1370	T2430	T2414	punct	.,are
R1371	T2432	T2433	nsubj	Pairs,dimerise
R1372	T2434	T2432	prep	of,Pairs
R1373	T2435	T2436	compound	CBS,domains
R1374	T2436	T2434	pobj	domains,of
R1375	T2437	T2438	aux	to,form
R1376	T2438	T2433	advcl	form,dimerise
R1377	T2439	T2440	det	a,domain
R1378	T2440	T2438	dobj	domain,form
R1379	T2441	T2440	amod	stable,domain
R138	T369	T367	pobj	Domain,within
R1380	T2442	T2440	amod	globular,domain
R1381	T2443	T2444	punct	[,8
R1382	T2444	T2433	parataxis	8,dimerise
R1383	T2445	T2444	punct	],8
R1384	T2446	T2433	punct	.,dimerise
R1385	T2448	T2449	nsubj	DUF21,is
R1386	T2450	T2451	punct	(,pfam01959.9
R1387	T2451	T2448	parataxis	pfam01959.9,DUF21
R1388	T2452	T2451	dep	CD,pfam01959.9
R1389	T2453	T2451	punct	: ,pfam01959.9
R139	T370	T369	amod	Ancient,Domain
R1390	T2454	T2451	punct	),pfam01959.9
R1391	T2455	T2456	det	a,domain
R1392	T2456	T2449	attr	domain,is
R1393	T2457	T2458	advmod	newly,defined
R1394	T2458	T2456	amod	defined,domain
R1395	T2459	T2456	prep	with,domain
R1396	T2460	T2461	amod	unknown,function
R1397	T2461	T2459	pobj	function,with
R1398	T2462	T2449	punct	.,is
R1399	T2464	T2465	det	This,domain
R14	T235	T232	cc	and,cloned
R140	T371	T369	amod	Conserved,Domain
R1400	T2465	T2466	nsubj	domain,is
R1401	T2467	T2468	det	a,region
R1402	T2468	T2466	attr	region,is
R1403	T2469	T2468	amod	transmembrane,region
R1404	T2470	T2468	cc	and,region
R1405	T2471	T2468	conj	found,region
R1406	T2472	T2473	aux	to,located
R1407	T2473	T2471	xcomp	located,found
R1408	T2474	T2473	auxpass	be,located
R1409	T2475	T2473	prep	in,located
R141	T372	T369	punct	(,Domain
R1410	T2476	T2477	det	the,terminus
R1411	T2477	T2475	pobj	terminus,in
R1412	T2478	T2477	compound	N,terminus
R1413	T2479	T2477	punct	-,terminus
R1414	T2480	T2477	prep	of,terminus
R1415	T2481	T2482	det	the,proteins
R1416	T2482	T2480	pobj	proteins,of
R1417	T2483	T2482	amod	adjacent,proteins
R1418	T2484	T2483	prep	to,adjacent
R1419	T2485	T2486	nummod	two,domains
R142	T373	T369	appos	ACD,Domain
R1420	T2486	T2484	pobj	domains,to
R1421	T2487	T2486	amod	intracellular,domains
R1422	T2488	T2486	compound	CBS,domains
R1423	T2489	T2466	punct	.,is
R1424	T2491	T2492	det	A,domain
R1425	T2492	T2496	nsubjpass	domain,found
R1426	T2493	T2494	npadvmod	cNMP,binding
R1427	T2494	T2492	amod	binding,domain
R1428	T2495	T2494	punct	-,binding
R1429	T2497	T2498	punct	(,domain
R143	T374	T358	punct	),conserved
R1430	T2498	T2492	parataxis	domain,domain
R1431	T2499	T2500	amod	cyclic,monophosphate
R1432	T2500	T2498	nmod	monophosphate,domain
R1433	T2501	T2500	nmod	nucleotide,monophosphate
R1434	T2502	T2500	punct	-,monophosphate
R1435	T2503	T2500	punct	-,monophosphate
R1436	T2504	T2500	amod	binding,monophosphate
R1437	T2505	T2498	punct	),domain
R1438	T2506	T2496	auxpass	was,found
R1439	T2507	T2496	prep	in,found
R144	T375	T358	auxpass	are,conserved
R1440	T2508	T2509	det	all,members
R1441	T2509	T2507	pobj	members,in
R1442	T2510	T2509	compound	Acdp,members
R1443	T2511	T2496	punct	.,found
R1444	T2513	T2514	prep	In,contains
R1445	T2515	T2513	pobj	addition,In
R1446	T2516	T2514	punct	", ",contains
R1447	T2517	T2514	nsubj	Acdp1,contains
R1448	T2518	T2519	det	an,region
R1449	T2519	T2514	dobj	region,contains
R145	T376	T358	advmod	highly,conserved
R1450	T2520	T2521	npadvmod	Alanine,rich
R1451	T2521	T2519	amod	rich,region
R1452	T2522	T2521	punct	-,rich
R1453	T2523	T2524	punct	(,AAAAAAAAA
R1454	T2524	T2519	parataxis	AAAAAAAAA,region
R1455	T2525	T2524	dep	2,AAAAAAAAA
R1456	T2526	T2527	punct	–,10
R1457	T2527	T2525	prep	10,2
R1458	T2528	T2524	punct	: ,AAAAAAAAA
R1459	T2529	T2524	punct	),AAAAAAAAA
R146	T377	T358	prep	in,conserved
R1460	T2530	T2519	punct	", ",region
R1461	T2531	T2532	det	a,region
R1462	T2532	T2519	conj	region,region
R1463	T2533	T2534	npadvmod	Leucine,rich
R1464	T2534	T2532	amod	rich,region
R1465	T2535	T2534	punct	-,rich
R1466	T2536	T2537	punct	(,SGLRLSLLSLDPVELRVL
R1467	T2537	T2532	parataxis	SGLRLSLLSLDPVELRVL,region
R1468	T2538	T2537	dep	204,SGLRLSLLSLDPVELRVL
R1469	T2539	T2540	punct	–,257
R147	T378	T379	amod	many,species
R1470	T2540	T2538	prep	257,204
R1471	T2541	T2537	punct	: ,SGLRLSLLSLDPVELRVL
R1472	T2542	T2537	compound	LLRVRPRLYGPGGDLLPPAWLRALGALLLLALSALF,SGLRLSLLSLDPVELRVL
R1473	T2543	T2537	punct	),SGLRLSLLSLDPVELRVL
R1474	T2544	T2532	punct	", ",region
R1475	T2545	T2546	det	a,region
R1476	T2546	T2532	conj	region,region
R1477	T2547	T2548	npadvmod	Proline,rich
R1478	T2548	T2546	amod	rich,region
R1479	T2549	T2548	punct	-,rich
R148	T379	T377	pobj	species,in
R1480	T2550	T2551	punct	(,PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1481	T2551	T2546	parataxis	PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP,region
R1482	T2552	T2551	dep	78,PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1483	T2553	T2554	punct	–,130
R1484	T2554	T2552	prep	130,78
R1485	T2555	T2551	punct	: ,PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1486	T2556	T2551	compound	PGPPVPAAPVPAPSLA,PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1487	T2557	T2551	punct	),PGENGTGDWAPRLVFIEEPPGAGGAAPSAVPTRPPGP
R1488	T2558	T2546	punct	", ",region
R1489	T2559	T2546	cc	and,region
R149	T380	T379	amod	different,species
R1490	T2560	T2561	nummod	two,sites
R1491	T2561	T2546	conj	sites,region
R1492	T2562	T2561	compound	amidation,sites
R1493	T2563	T2564	punct	(,SGRK
R1494	T2564	T2561	parataxis	SGRK,sites
R1495	T2565	T2566	dep	917,MGKK
R1496	T2566	T2564	dep	MGKK,SGRK
R1497	T2567	T2568	punct	–,920
R1498	T2568	T2565	prep	920,917
R1499	T2569	T2566	punct	: ,MGKK
R15	T236	T232	conj	characterized,cloned
R150	T381	T379	amod	taxonomic,species
R1500	T2570	T2564	punct	;,SGRK
R1501	T2571	T2564	dep	926,SGRK
R1502	T2572	T2573	punct	–,929
R1503	T2573	T2571	prep	929,926
R1504	T2574	T2564	punct	: ,SGRK
R1505	T2575	T2564	punct	),SGRK
R1506	T2576	T2514	punct	.,contains
R1507	T2578	T2579	nsubj	Acdp2,has
R1508	T2580	T2581	det	a,region
R1509	T2581	T2579	dobj	region,has
R151	T382	T358	punct	.,conserved
R1510	T2582	T2583	npadvmod	glycine,rich
R1511	T2583	T2581	amod	rich,region
R1512	T2584	T2583	punct	-,rich
R1513	T2585	T2586	punct	(,GAGGSGSASGTVGGKGGAGVAG
R1514	T2586	T2581	parataxis	GAGGSGSASGTVGGKGGAGVAG,region
R1515	T2587	T2586	dep	201,GAGGSGSASGTVGGKGGAGVAG
R1516	T2588	T2589	punct	–,222
R1517	T2589	T2587	prep	222,201
R1518	T2590	T2586	punct	: ,GAGGSGSASGTVGGKGGAGVAG
R1519	T2591	T2586	punct	),GAGGSGSASGTVGGKGGAGVAG
R152	T384	T385	advmod	Particularly,showed
R1520	T2592	T2579	punct	.,has
R1521	T2594	T2595	nsubj	Acdp3,possesses
R1522	T2596	T2597	det	a,region
R1523	T2597	T2595	dobj	region,possesses
R1524	T2598	T2597	amod	large,region
R1525	T2599	T2600	npadvmod	alanine,rich
R1526	T2600	T2597	amod	rich,region
R1527	T2601	T2600	punct	-,rich
R1528	T2602	T2603	punct	(,2
R1529	T2603	T2597	parataxis	2,region
R153	T386	T385	punct	", ",showed
R1530	T2604	T2605	punct	–,261
R1531	T2605	T2603	prep	261,2
R1532	T2606	T2603	punct	),2
R1533	T2607	T2597	cc	and,region
R1534	T2608	T2609	det	a,region
R1535	T2609	T2597	conj	region,region
R1536	T2610	T2609	amod	large,region
R1537	T2611	T2612	npadvmod	leucine,rich
R1538	T2612	T2609	amod	rich,region
R1539	T2613	T2612	punct	-,rich
R154	T387	T388	compound	Acdp,proteins
R1540	T2614	T2615	punct	(,201
R1541	T2615	T2609	parataxis	201,region
R1542	T2616	T2617	punct	–,299
R1543	T2617	T2615	prep	299,201
R1544	T2618	T2615	punct	),201
R1545	T2619	T2595	punct	.,possesses
R1546	T2621	T2622	nsubj	Acdp4,contains
R1547	T2623	T2624	det	a,pattern
R1548	T2624	T2622	dobj	pattern,contains
R1549	T2625	T2624	compound	leucine,pattern
R155	T388	T385	nsubj	proteins,showed
R1550	T2626	T2624	compound	zipper,pattern
R1551	T2627	T2628	punct	(,LVMVLLVLSGIFSGLNLGLMAL
R1552	T2628	T2624	parataxis	LVMVLLVLSGIFSGLNLGLMAL,pattern
R1553	T2629	T2628	dep	185,LVMVLLVLSGIFSGLNLGLMAL
R1554	T2630	T2631	punct	–,206
R1555	T2631	T2629	prep	206,185
R1556	T2632	T2628	punct	: ,LVMVLLVLSGIFSGLNLGLMAL
R1557	T2633	T2628	punct	),LVMVLLVLSGIFSGLNLGLMAL
R1558	T2634	T2624	cc	and,pattern
R1559	T2635	T2636	det	an,site
R156	T389	T390	advmod	very,strong
R1560	T2636	T2624	conj	site,pattern
R1561	T2637	T2636	compound	amidation,site
R1562	T2638	T2639	punct	(,GGRR
R1563	T2639	T2636	parataxis	GGRR,site
R1564	T2640	T2639	dep	7,GGRR
R1565	T2641	T2642	punct	–,10
R1566	T2642	T2640	prep	10,7
R1567	T2643	T2639	punct	: ,GGRR
R1568	T2644	T2639	punct	),GGRR
R1569	T2645	T2622	punct	.,contains
R157	T390	T391	amod	strong,homologies
R1572	T2862	T2863	compound	Antibody,production
R1573	T2864	T2863	punct	", ",production
R1574	T2865	T2866	compound	Western,results
R1575	T2866	T2863	conj	results,production
R1576	T2867	T2866	cc	and,results
R1577	T2868	T2869	amod	subcellular,localization
R1578	T2869	T2866	conj	localization,results
R1579	T2871	T2872	nsubjpass	Peptides,synthesized
R158	T391	T385	dobj	homologies,showed
R1580	T2873	T2871	prep	from,Peptides
R1581	T2874	T2875	nmod	Acdp1,terminuses
R1582	T2875	T2873	pobj	terminuses,from
R1583	T2876	T2875	nmod	N,terminuses
R1584	T2877	T2876	punct	-,N
R1585	T2878	T2876	punct	(,N
R1586	T2879	T2876	appos	TSFLLRVYFQPGPPATAAPVPSPT,N
R1587	T2880	T2876	punct	),N
R1588	T2881	T2876	cc	and,N
R1589	T2882	T2876	conj	C,N
R159	T392	T391	compound	AA,homologies
R1590	T2883	T2882	punct	-,C
R1591	T2884	T2882	punct	(,C
R1592	T2885	T2882	appos	TQQLTLSPAAVPTR,C
R1593	T2886	T2875	punct	),terminuses
R1594	T2887	T2871	punct	", ",Peptides
R1595	T2888	T2889	amod	conserved,peptide
R1596	T2889	T2871	appos	peptide,Peptides
R1597	T2890	T2889	prep	from,peptide
R1598	T2891	T2892	compound	ACD,domain
R1599	T2892	T2890	pobj	domain,from
R16	T237	T238	det	a,family
R160	T393	T391	prep	to,homologies
R1600	T2893	T2892	prep	of,domain
R1601	T2894	T2893	pobj	Acdp1,of
R1602	T2895	T2894	punct	(,Acdp1
R1603	T2896	T2894	appos	HNIVDILFVKDLAFVDPDDCTPLLTVTRF,Acdp1
R1604	T2897	T2872	punct	),synthesized
R1605	T2898	T2872	auxpass	were,synthesized
R1606	T2899	T2872	advmod	commercially,synthesized
R1607	T2900	T2901	punct	(,Genosys
R1608	T2901	T2872	parataxis	Genosys,synthesized
R1609	T2902	T2901	compound	Sigma,Genosys
R161	T394	T395	det	the,protein
R1610	T2903	T2901	punct	),Genosys
R1611	T2904	T2872	punct	.,synthesized
R1612	T2906	T2907	det	These,sites
R1613	T2907	T2909	nsubjpass	sites,predicted
R1614	T2908	T2907	amod	antigenic,sites
R1615	T2910	T2909	auxpass	were,predicted
R1616	T2911	T2909	prep	by,predicted
R1617	T2912	T2911	pobj	software,by
R1618	T2913	T2912	prep	from,software
R1619	T2914	T2915	compound	Sigma,Genosys
R162	T395	T393	pobj	protein,to
R1620	T2915	T2913	pobj	Genosys,from
R1621	T2916	T2909	cc	and,predicted
R1622	T2917	T2918	amod	polyclonal,antibodies
R1623	T2918	T2919	nsubjpass	antibodies,produced
R1624	T2919	T2909	conj	produced,predicted
R1625	T2920	T2918	prep	for,antibodies
R1626	T2921	T2922	det	each,peptide
R1627	T2922	T2920	pobj	peptide,for
R1628	T2923	T2919	auxpass	were,produced
R1629	T2924	T2919	prep	by,produced
R163	T396	T395	compound	bacteria,protein
R1630	T2925	T2924	pcomp	immunizing,by
R1631	T2926	T2925	dobj	rabbits,immunizing
R1632	T2927	T2919	punct	.,produced
R1633	T2929	T2930	aux	To,test
R1634	T2930	T2931	advcl	test,conducted
R1635	T2932	T2933	det	the,specificity
R1636	T2933	T2930	dobj	specificity,test
R1637	T2934	T2933	prep	of,specificity
R1638	T2935	T2936	det	the,antibodies
R1639	T2936	T2934	pobj	antibodies,of
R164	T397	T395	compound	CorC,protein
R1640	T2937	T2931	punct	", ",conducted
R1641	T2938	T2931	nsubj	we,conducted
R1642	T2939	T2940	compound	Western,blot
R1643	T2940	T2942	compound	blot,analysis
R1644	T2941	T2940	punct	-,blot
R1645	T2942	T2931	dobj	analysis,conducted
R1646	T2943	T2942	prep	of,analysis
R1647	T2944	T2945	compound	mouse,brain
R1648	T2945	T2946	compound	brain,tissue
R1649	T2946	T2947	compound	tissue,extracts
R165	T398	T399	punct	(,identity
R1650	T2947	T2943	pobj	extracts,of
R1651	T2948	T2931	punct	.,conducted
R1652	T2950	T2951	mark	As,shown
R1653	T2951	T2952	advcl	shown,detected
R1654	T2953	T2951	prep	in,shown
R1655	T2954	T2953	pobj	Fig.,in
R1656	T2955	T2954	nummod	6A,Fig.
R1657	T2956	T2952	punct	", ",detected
R1658	T2957	T2958	det	the,antibody
R1659	T2958	T2952	nsubj	antibody,detected
R166	T399	T395	parataxis	identity,protein
R1660	T2959	T2958	acl	produced,antibody
R1661	T2960	T2959	prep	by,produced
R1662	T2961	T2962	nmod	C,peptide
R1663	T2962	T2960	pobj	peptide,by
R1664	T2963	T2961	punct	-,C
R1665	T2964	T2961	amod	terminal,C
R1666	T2965	T2952	advmod	specifically,detected
R1667	T2966	T2952	dobj	Acdp1,detected
R1668	T2967	T2968	punct	(,lane
R1669	T2968	T2952	parataxis	lane,detected
R167	T400	T401	nummod	35,%
R1670	T2969	T2968	nummod	3,lane
R1671	T2970	T2968	punct	),lane
R1672	T2971	T2952	punct	.,detected
R1673	T2973	T2974	det	The,antibody
R1674	T2974	T2975	nsubj	antibody,recognized
R1675	T2976	T2974	acl	generated,antibody
R1676	T2977	T2976	agent	by,generated
R1677	T2978	T2979	nmod	N,peptide
R1678	T2979	T2977	pobj	peptide,by
R1679	T2980	T2978	punct	-,N
R168	T401	T399	compound	%,identity
R1680	T2981	T2978	amod	terminal,N
R1681	T2982	T2975	dobj	Acdp4,recognized
R1682	T2983	T2975	prep	in,recognized
R1683	T2984	T2983	pobj	addition,in
R1684	T2985	T2984	prep	to,addition
R1685	T2986	T2985	pobj	Acdp1,to
R1686	T2987	T2975	punct	", ",recognized
R1687	T2988	T2989	mark	although,was
R1688	T2989	T2975	advcl	was,recognized
R1689	T2990	T2991	det	the,reactivity
R169	T402	T399	compound	AA,identity
R1690	T2991	T2989	nsubj	reactivity,was
R1691	T2992	T2991	prep	to,reactivity
R1692	T2993	T2992	pobj	latter,to
R1693	T2994	T2995	advmod	significantly,higher
R1694	T2995	T2989	acomp	higher,was
R1695	T2996	T2997	punct	(,lane
R1696	T2997	T2975	parataxis	lane,recognized
R1697	T2998	T2997	dep	Fig.,lane
R1698	T2999	T2998	nummod	6A,Fig.
R1699	T3000	T2997	punct	", ",lane
R17	T238	T236	dobj	family,characterized
R170	T403	T399	prep	with,identity
R1700	T3001	T2997	nummod	2,lane
R1701	T3002	T2997	punct	),lane
R1702	T3003	T2975	punct	.,recognized
R1703	T3005	T3006	mark	As,expected
R1704	T3006	T3007	advcl	expected,detected
R1705	T3008	T3007	punct	", ",detected
R1706	T3009	T3010	det	the,antibody
R1707	T3010	T3007	nsubj	antibody,detected
R1708	T3011	T3010	acl	produced,antibody
R1709	T3012	T3011	agent	by,produced
R171	T404	T405	nummod	55,%
R1710	T3013	T3014	det	the,peptide
R1711	T3014	T3012	pobj	peptide,by
R1712	T3015	T3014	amod	conserved,peptide
R1713	T3016	T3014	compound	sequence,peptide
R1714	T3017	T3018	det	all,proteins
R1715	T3018	T3007	dobj	proteins,detected
R1716	T3019	T3018	compound	Acdp,proteins
R1717	T3020	T3021	punct	(,lane
R1718	T3021	T3007	parataxis	lane,detected
R1719	T3022	T3021	dep	Fig.,lane
R172	T405	T406	compound	%,homology
R1720	T3023	T3022	nummod	6A,Fig.
R1721	T3024	T3021	punct	", ",lane
R1722	T3025	T3021	nummod	1,lane
R1723	T3026	T3021	punct	),lane
R1724	T3027	T3007	punct	.,detected
R1725	T3029	T3030	aux	To,determine
R1726	T3030	T3032	advcl	determine,analyzed
R1727	T3031	T3030	advmod	further,determine
R1728	T3033	T3034	det	the,specificity
R1729	T3034	T3030	dobj	specificity,determine
R173	T406	T403	pobj	homology,with
R1730	T3035	T3034	prep	of,specificity
R1731	T3036	T3037	det	the,antibody
R1732	T3037	T3035	pobj	antibody,of
R1733	T3038	T3034	prep	against,specificity
R1734	T3039	T3040	det	the,terminus
R1735	T3040	T3038	pobj	terminus,against
R1736	T3041	T3040	compound	Acdp1,terminus
R1737	T3042	T3040	compound	C,terminus
R1738	T3043	T3040	punct	-,terminus
R1739	T3044	T3032	punct	", ",analyzed
R174	T407	T399	punct	),identity
R1740	T3045	T3032	nsubj	we,analyzed
R1741	T3046	T3032	dobj	extracts,analyzed
R1742	T3047	T3046	prep	of,extracts
R1743	T3048	T3049	nmod	HEK293,cells
R1744	T3049	T3047	pobj	cells,of
R1745	T3050	T3048	punct	", ",HEK293
R1746	T3051	T3048	conj	3T3,HEK293
R1747	T3052	T3051	cc	and,3T3
R1748	T3053	T3051	conj	PC12,3T3
R1749	T3054	T3032	punct	.,analyzed
R175	T408	T395	punct	", ",protein
R1750	T3056	T3057	det	The,results
R1751	T3057	T3058	nsubjpass	results,shown
R1752	T3059	T3058	auxpass	are,shown
R1753	T3060	T3058	prep	in,shown
R1754	T3061	T3060	pobj	Fig.,in
R1755	T3062	T3061	nummod	6B,Fig.
R1756	T3063	T3058	punct	.,shown
R1757	T3065	T3066	advmod	Apparently,detected
R1758	T3067	T3066	punct	", ",detected
R1759	T3068	T3069	det	this,antibody
R176	T409	T410	dep	which,involved
R1760	T3069	T3066	nsubj	antibody,detected
R1761	T3070	T3071	det	a,band
R1762	T3071	T3066	dobj	band,detected
R1763	T3072	T3071	amod	specific,band
R1764	T3073	T3071	prep	of,band
R1765	T3074	T3073	pobj	Acdp1,of
R1766	T3075	T3066	prep	in,detected
R1767	T3076	T3077	det	all,lysates
R1768	T3077	T3075	pobj	lysates,in
R1769	T3078	T3077	compound	cell,lysates
R177	T410	T395	relcl	involved,protein
R1770	T3079	T3066	punct	.,detected
R1771	T3081	T3082	prep	Of,are
R1772	T3083	T3081	pobj	note,Of
R1773	T3084	T3082	punct	", ",are
R1774	T3085	T3082	dep	shown,are
R1775	T3086	T3085	prep	in,shown
R1776	T3087	T3086	pobj	Fig.,in
R1777	T3088	T3087	nummod	6B,Fig.
R1778	T3089	T3082	nsubj	signals,are
R1779	T3090	T3089	prep	of,signals
R178	T411	T410	auxpass	is,involved
R1780	T3091	T3092	nummod	10,μg
R1781	T3092	T3093	compound	μg,extracts
R1782	T3093	T3090	pobj	extracts,of
R1783	T3094	T3093	prep	of,extracts
R1784	T3095	T3096	compound	HEK293,lysates
R1785	T3096	T3094	pobj	lysates,of
R1786	T3097	T3096	compound	cell,lysates
R1787	T3098	T3093	punct	", ",extracts
R1788	T3099	T3100	nummod	100,μg
R1789	T3100	T3101	compound	μg,extracts
R179	T412	T410	prep	in,involved
R1790	T3101	T3093	appos	extracts,extracts
R1791	T3102	T3101	prep	of,extracts
R1792	T3103	T3104	nmod	3T3,lysates
R1793	T3104	T3102	pobj	lysates,of
R1794	T3105	T3103	cc	and,3T3
R1795	T3106	T3103	conj	PC12,3T3
R1796	T3107	T3104	compound	cell,lysates
R1797	T3108	T3082	punct	.,are
R1798	T3110	T3111	advmod	Thus,vary
R1799	T3112	T3111	punct	", ",vary
R18	T239	T238	amod	novel,family
R180	T413	T414	nmod	magnesium,efflux
R1800	T3113	T3114	det	the,levels
R1801	T3114	T3111	nsubj	levels,vary
R1802	T3115	T3114	compound	expression,levels
R1803	T3116	T3114	prep	of,levels
R1804	T3117	T3116	pobj	Acdp1,of
R1805	T3118	T3114	prep	in,levels
R1806	T3119	T3120	det	these,types
R1807	T3120	T3118	pobj	types,in
R1808	T3121	T3120	compound	cell,types
R1809	T3122	T3123	det	a,lot
R181	T414	T412	pobj	efflux,in
R1810	T3123	T3111	dobj	lot,vary
R1811	T3124	T3111	punct	", ",vary
R1812	T3125	T3111	prep	with,vary
R1813	T3126	T3127	det	the,expression
R1814	T3127	T3125	pobj	expression,with
R1815	T3128	T3127	amod	highest,expression
R1816	T3129	T3127	prep	in,expression
R1817	T3130	T3131	compound	HEK293,cells
R1818	T3131	T3129	pobj	cells,in
R1819	T3132	T3111	punct	.,vary
R182	T415	T413	cc	and,magnesium
R1820	T3134	T3135	advmod	Nevertheless,support
R1821	T3136	T3135	punct	", ",support
R1822	T3137	T3138	det	these,results
R1823	T3138	T3135	nsubj	results,support
R1824	T3139	T3138	compound	immunoblot,results
R1825	T3140	T3141	poss	our,analysis
R1826	T3141	T3135	dobj	analysis,support
R1827	T3142	T3141	prep	of,analysis
R1828	T3143	T3144	compound	brain,extracts
R1829	T3144	T3142	pobj	extracts,of
R183	T416	T413	conj	cobalt,magnesium
R1830	T3145	T3144	compound	tissue,extracts
R1831	T3146	T3147	mark	that,recognizes
R1832	T3147	T3141	acl	recognizes,analysis
R1833	T3148	T3149	det	the,antibody
R1834	T3149	T3147	nsubj	antibody,recognizes
R1835	T3150	T3149	prep	against,antibody
R1836	T3151	T3152	compound	Acdp1,terminus
R1837	T3152	T3150	pobj	terminus,against
R1838	T3153	T3152	compound	C,terminus
R1839	T3154	T3152	punct	-,terminus
R184	T417	T385	punct	.,showed
R1840	T3155	T3147	advmod	specifically,recognizes
R1841	T3156	T3147	dobj	Acdp,recognizes
R1842	T3157	T3156	punct	-,Acdp
R1843	T3158	T3156	nummod	1,Acdp
R1844	T3159	T3135	punct	.,support
R1845	T3161	T3162	det	The,specificity
R1846	T3162	T3163	nsubj	specificity,suggests
R1847	T3164	T3162	prep	of,specificity
R1848	T3165	T3166	det	the,antibody
R1849	T3166	T3164	pobj	antibody,of
R185	T419	T420	det	The,genes
R1850	T3167	T3166	compound	Acdp1,antibody
R1851	T3168	T3169	compound	C,terminus
R1852	T3169	T3166	compound	terminus,antibody
R1853	T3170	T3169	punct	-,terminus
R1854	T3171	T3172	det	the,possibility
R1855	T3172	T3163	dobj	possibility,suggests
R1856	T3173	T3172	prep	of,possibility
R1857	T3174	T3173	pcomp	using,of
R1858	T3175	T3174	dobj	it,using
R1859	T3176	T3177	aux	to,localize
R186	T420	T422	nsubjpass	genes,expressed
R1860	T3177	T3174	advcl	localize,using
R1861	T3178	T3177	dobj	Acdp,localize
R1862	T3179	T3178	punct	-,Acdp
R1863	T3180	T3178	nummod	1,Acdp
R1864	T3181	T3177	prep	within,localize
R1865	T3182	T3181	pobj	cells,within
R1866	T3183	T3163	punct	.,suggests
R1867	T3185	T3186	mark	Since,revealed
R1868	T3186	T3189	advcl	revealed,examined
R1869	T3187	T3188	compound	Northern,blot
R187	T421	T420	compound	Acdp,genes
R1870	T3188	T3186	nsubj	blot,revealed
R1871	T3190	T3191	advmod	almost,exclusive
R1872	T3191	T3192	amod	exclusive,expression
R1873	T3192	T3186	dobj	expression,revealed
R1874	T3193	T3192	prep	of,expression
R1875	T3194	T3193	pobj	Acdp1,of
R1876	T3195	T3186	prep	in,revealed
R1877	T3196	T3197	det	the,brain
R1878	T3197	T3195	pobj	brain,in
R1879	T3198	T3189	punct	", ",examined
R188	T423	T422	auxpass	are,expressed
R1880	T3199	T3189	nsubj	we,examined
R1881	T3200	T3201	poss	its,localization
R1882	T3201	T3189	dobj	localization,examined
R1883	T3202	T3201	amod	subcellular,localization
R1884	T3203	T3189	prep	in,examined
R1885	T3204	T3205	compound	hippocampus,neurons
R1886	T3205	T3203	pobj	neurons,in
R1887	T3206	T3205	acl	isolated,neurons
R1888	T3207	T3206	prep	from,isolated
R1889	T3208	T3209	compound	mouse,embryos
R189	T424	T422	advmod	widely,expressed
R1890	T3209	T3207	pobj	embryos,from
R1891	T3210	T3189	punct	.,examined
R1892	T3212	T3213	det	The,neurons
R1893	T3213	T3214	nsubjpass	neurons,cultured
R1894	T3215	T3214	auxpass	were,cultured
R1895	T3216	T3214	prep	on,cultured
R1896	T3217	T3218	compound	glass,coverslips
R1897	T3218	T3216	pobj	coverslips,on
R1898	T3219	T3218	acl	coated,coverslips
R1899	T3220	T3219	prep	with,coated
R19	T240	T238	compound	gene,family
R190	T425	T422	prep	in,expressed
R1900	T3221	T3222	det	a,monolayer
R1901	T3222	T3220	pobj	monolayer,with
R1902	T3223	T3222	amod	confluent,monolayer
R1903	T3224	T3222	prep	of,monolayer
R1904	T3225	T3226	nmod	mouse,astrocytes
R1905	T3226	T3224	pobj	astrocytes,of
R1906	T3227	T3226	amod	cortical,astrocytes
R1907	T3228	T3214	prep	in,cultured
R1908	T3229	T3228	pobj	dishes,in
R1909	T3230	T3214	punct	.,cultured
R191	T426	T427	det	all,tissues
R1910	T3232	T3233	nsubj	Immunostaining,using
R1911	T3234	T3233	aux	was,using
R1912	T3235	T3236	det	the,antibody
R1913	T3236	T3233	dobj	antibody,using
R1914	T3237	T3236	nmod	Acdp1,antibody
R1915	T3238	T3239	compound	C,terminus
R1916	T3239	T3241	npadvmod	terminus,specific
R1917	T3240	T3239	punct	-,terminus
R1918	T3241	T3236	amod	specific,antibody
R1919	T3242	T3233	punct	.,using
R192	T427	T425	pobj	tissues,in
R1920	T3244	T3245	amod	Confocal,imaging
R1921	T3245	T3246	nsubj	imaging,revealed
R1922	T3247	T3248	mark	that,localized
R1923	T3248	T3246	ccomp	localized,revealed
R1924	T3249	T3248	nsubjpass	Acdp1,localized
R1925	T3250	T3248	auxpass	is,localized
R1926	T3251	T3248	advmod	predominantly,localized
R1927	T3252	T3248	prep	on,localized
R1928	T3253	T3254	det	the,membrane
R1929	T3254	T3252	pobj	membrane,on
R193	T428	T427	acl	tested,tissues
R1930	T3255	T3254	compound	plasma,membrane
R1931	T3256	T3246	punct	.,revealed
R1932	T3258	T3259	det	A,series
R1933	T3259	T3260	nsubj	series,showed
R1934	T3261	T3259	prep	of,series
R1935	T3262	T3261	pobj	sections,of
R1936	T3263	T3262	prep	of,sections
R1937	T3264	T3265	det	a,cell
R1938	T3265	T3263	pobj	cell,of
R1939	T3266	T3259	prep	at,series
R194	T429	T422	prep	except,expressed
R1940	T3267	T3268	det	the,thickness
R1941	T3268	T3266	pobj	thickness,at
R1942	T3269	T3268	prep	of,thickness
R1943	T3270	T3271	nummod	0.5,micrometer
R1944	T3271	T3269	pobj	micrometer,of
R1945	T3272	T3260	advmod	clearly,showed
R1946	T3273	T3274	compound	membrane,location
R1947	T3274	T3260	dobj	location,showed
R1948	T3275	T3274	prep	of,location
R1949	T3276	T3277	compound	Acdp1,immunoreactivity
R195	T430	T429	prep	for,except
R1950	T3277	T3275	pobj	immunoreactivity,of
R1951	T3278	T3277	punct	-,immunoreactivity
R1952	T3279	T3280	punct	(,Fig.
R1953	T3280	T3277	parataxis	Fig.,immunoreactivity
R1954	T3281	T3280	nummod	7,Fig.
R1955	T3282	T3280	punct	),Fig.
R1956	T3283	T3260	punct	", ",showed
R1957	T3284	T3285	dep	which,is
R1958	T3285	T3260	advcl	is,showed
R1959	T3286	T3285	acomp	consistent,is
R196	T431	T430	pobj	Acdp1,for
R1960	T3287	T3286	prep	with,consistent
R1961	T3288	T3289	det	the,observation
R1962	T3289	T3287	pobj	observation,with
R1963	T3290	T3289	prep	of,observation
R1964	T3291	T3292	compound	transmembrane,domains
R1965	T3292	T3290	pobj	domains,of
R1966	T3293	T3289	prep	within,observation
R1967	T3294	T3295	det	the,proteins
R1968	T3295	T3293	pobj	proteins,within
R1969	T3296	T3295	compound	Acdp,proteins
R197	T432	T431	punct	", ",Acdp1
R1970	T3297	T3260	punct	.,showed
R1973	T3529	T3530	mark	Although,elucidated
R1974	T3530	T3542	advcl	elucidated,suggest
R1975	T3531	T3532	det	the,functions
R1976	T3532	T3530	nsubjpass	functions,elucidated
R1977	T3533	T3532	amod	exact,functions
R1978	T3534	T3532	prep	of,functions
R1979	T3535	T3536	det	the,family
R198	T433	T434	dep	which,expressed
R1980	T3536	T3534	pobj	family,of
R1981	T3537	T3536	compound	ACDP,family
R1982	T3538	T3536	compound	gene,family
R1983	T3539	T3530	auxpass	are,elucidated
R1984	T3540	T3530	neg	not,elucidated
R1985	T3541	T3530	advmod	yet,elucidated
R1986	T3543	T3542	punct	", ",suggest
R1987	T3544	T3545	amod	several,lines
R1988	T3545	T3542	nsubj	lines,suggest
R1989	T3546	T3545	prep	of,lines
R199	T434	T431	relcl	expressed,Acdp1
R1990	T3547	T3546	pobj	evidence,of
R1991	T3548	T3549	mark	that,play
R1992	T3549	T3542	ccomp	play,suggest
R1993	T3550	T3551	compound	ACDP,genes
R1994	T3551	T3549	nsubj	genes,play
R1995	T3552	T3549	aux	may,play
R1996	T3553	T3554	det	an,role
R1997	T3554	T3549	dobj	role,play
R1998	T3555	T3554	amod	important,role
R1999	T3556	T3549	prep	in,play
R20	T241	T238	acl	named,family
R200	T435	T434	auxpass	is,expressed
R2000	T3557	T3558	amod	biological,processes
R2001	T3558	T3556	pobj	processes,in
R2002	T3559	T3542	punct	.,suggest
R2003	T3561	T3562	advmod	First,conserved
R2004	T3562	T3568	ccomp	conserved,expressed
R2005	T3563	T3562	punct	", ",conserved
R2006	T3564	T3565	det	these,genes
R2007	T3565	T3562	nsubjpass	genes,conserved
R2008	T3566	T3562	auxpass	are,conserved
R2009	T3567	T3562	advmod	evolutionarily,conserved
R201	T436	T434	advmod	only,expressed
R2010	T3569	T3562	prep	in,conserved
R2011	T3570	T3571	amod	many,species
R2012	T3571	T3569	pobj	species,in
R2013	T3572	T3573	advmod	phylogenetically,divergent
R2014	T3573	T3571	amod	divergent,species
R2015	T3574	T3568	punct	;,expressed
R2016	T3575	T3576	advmod	Second,are
R2017	T3576	T3568	ccomp	are,expressed
R2018	T3577	T3576	punct	", ",are
R2019	T3578	T3579	amod	multiple,genes
R202	T437	T434	advmod	highly,expressed
R2020	T3579	T3576	nsubj	genes,are
R2021	T3580	T3576	acomp	present,are
R2022	T3581	T3576	prep	in,are
R2023	T3582	T3583	det	a,species
R2024	T3583	T3581	pobj	species,in
R2025	T3584	T3568	punct	;,expressed
R2026	T3585	T3568	advmod	Third,expressed
R2027	T3586	T3568	punct	", ",expressed
R2028	T3587	T3588	det	these,genes
R2029	T3588	T3568	nsubjpass	genes,expressed
R203	T438	T434	prep	in,expressed
R2030	T3589	T3568	auxpass	are,expressed
R2031	T3590	T3568	advmod	ubiquitously,expressed
R2032	T3591	T3568	prep	throughout,expressed
R2033	T3592	T3593	nmod	development,tissues
R2034	T3593	T3591	pobj	tissues,throughout
R2035	T3594	T3592	cc	and,development
R2036	T3595	T3592	conj	adult,development
R2037	T3596	T3597	punct	(,data
R2038	T3597	T3568	meta	data,expressed
R2039	T3598	T3597	amod	unpublished,data
R204	T439	T440	det	the,brain
R2040	T3599	T3597	punct	),data
R2041	T3600	T3568	punct	.,expressed
R2042	T3602	T3603	det	The,cloning
R2043	T3603	T3604	nsubj	cloning,are
R2044	T3605	T3603	cc	and,cloning
R2045	T3606	T3603	conj	characterization,cloning
R2046	T3607	T3603	prep	of,cloning
R2047	T3608	T3609	det	the,family
R2048	T3609	T3607	pobj	family,of
R2049	T3610	T3609	compound	mouse,family
R205	T440	T438	pobj	brain,in
R2050	T3611	T3609	compound	Acdp,family
R2051	T3612	T3609	compound	gene,family
R2052	T3613	T3614	det	a,step
R2053	T3614	T3604	attr	step,are
R2054	T3615	T3616	advmod	very,important
R2055	T3616	T3614	amod	important,step
R2056	T3617	T3614	prep	towards,step
R2057	T3618	T3619	det	the,elucidation
R2058	T3619	T3617	pobj	elucidation,towards
R2059	T3620	T3619	prep	of,elucidation
R206	T441	T442	mark	with,levels
R2060	T3621	T3622	det	the,functions
R2061	T3622	T3620	pobj	functions,of
R2062	T3623	T3622	prep	of,functions
R2063	T3624	T3625	det	this,family
R2064	T3625	T3623	pobj	family,of
R2065	T3626	T3625	amod	multigene,family
R2066	T3627	T3604	punct	.,are
R2067	T3629	T3630	compound	Sequence,analyses
R2068	T3630	T3632	nsubj	analyses,revealed
R2069	T3631	T3630	compound	homology,analyses
R207	T442	T434	advcl	levels,expressed
R2070	T3633	T3634	mark	that,shared
R2071	T3634	T3632	ccomp	shared,revealed
R2072	T3635	T3636	compound	Acdp,proteins
R2073	T3636	T3634	nsubj	proteins,shared
R2074	T3637	T3638	advmod	very,strong
R2075	T3638	T3639	amod	strong,homologies
R2076	T3639	T3634	dobj	homologies,shared
R2077	T3640	T3639	compound	AA,homologies
R2078	T3641	T3639	prep	to,homologies
R2079	T3642	T3643	det	the,protein
R208	T443	T442	amod	low,levels
R2080	T3643	T3641	pobj	protein,to
R2081	T3644	T3643	compound	bacteria,protein
R2082	T3645	T3643	compound	CorC,protein
R2083	T3646	T3643	cc	and,protein
R2084	T3647	T3648	compound	yeast,protein
R2085	T3648	T3643	conj	protein,protein
R2086	T3649	T3648	compound	Amip3,protein
R2087	T3650	T3632	punct	.,revealed
R2088	T3652	T3653	nsubj	CorA,belong
R2089	T3654	T3652	punct	", ",CorA
R209	T444	T442	prep	of,levels
R2090	T3655	T3652	conj	B,CorA
R2091	T3656	T3655	punct	", ",B
R2092	T3657	T3655	conj	C,B
R2093	T3658	T3657	cc	and,C
R2094	T3659	T3657	conj	D,C
R2095	T3660	T3653	prep	to,belong
R2096	T3661	T3662	det	a,family
R2097	T3662	T3660	pobj	family,to
R2098	T3663	T3662	compound	protein,family
R2099	T3664	T3662	acl	involved,family
R21	T242	T243	amod	ancient,protein
R210	T445	T444	pobj	expression,of
R2100	T3665	T3664	prep	in,involved
R2101	T3666	T3667	preconj	both,influx
R2102	T3667	T3665	pobj	influx,in
R2103	T3668	T3667	cc	and,influx
R2104	T3669	T3667	conj	efflux,influx
R2105	T3670	T3667	prep	of,influx
R2106	T3671	T3670	pobj	magnesium,of
R2107	T3672	T3671	cc	and,magnesium
R2108	T3673	T3671	conj	cobalt,magnesium
R2109	T3674	T3653	punct	.,belong
R211	T446	T442	prep	in,levels
R2110	T3676	T3677	nsubjpass	It,shown
R2111	T3678	T3677	aux	has,shown
R2112	T3679	T3677	auxpass	been,shown
R2113	T3680	T3681	mark	that,confer
R2114	T3681	T3677	ccomp	confer,shown
R2115	T3682	T3683	compound	CorA,mutants
R2116	T3683	T3681	nsubj	mutants,confer
R2117	T3684	T3681	dobj	resistance,confer
R2118	T3685	T3684	prep	to,resistance
R2119	T3686	T3687	compound	cobalt,toxicity
R212	T447	T446	pobj	kidney,in
R2120	T3687	T3685	pobj	toxicity,to
R2121	T3688	T3689	punct	[,9
R2122	T3689	T3677	parataxis	9,shown
R2123	T3690	T3689	punct	],9
R2124	T3691	T3677	punct	.,shown
R2125	T3693	T3694	nsubj	Amip3,appears
R2126	T3695	T3696	aux	to,be
R2127	T3696	T3694	xcomp	be,appears
R2128	T3697	T3698	det	a,homologous
R2129	T3698	T3696	attr	homologous,be
R213	T448	T447	cc	and,kidney
R2130	T3699	T3698	prep	to,homologous
R2131	T3700	T3701	compound	CorC,protein
R2132	T3701	T3699	pobj	protein,to
R2133	T3702	T3703	dep	which,involved
R2134	T3703	T3701	relcl	involved,protein
R2135	T3704	T3703	auxpass	is,involved
R2136	T3705	T3703	prep	in,involved
R2137	T3706	T3705	pobj	efflux,in
R2138	T3707	T3706	prep	of,efflux
R2139	T3708	T3707	pobj	magnesium,of
R214	T449	T447	conj	testis,kidney
R2140	T3709	T3708	cc	and,magnesium
R2141	T3710	T3708	conj	cobalt,magnesium
R2142	T3711	T3703	prep	in,involved
R2143	T3712	T3711	pobj	bacteria,in
R2144	T3713	T3694	punct	.,appears
R2145	T3715	T3716	compound	Amip3,mutants
R2146	T3716	T3717	nsubj	mutants,confer
R2147	T3718	T3717	dobj	resistant,confer
R2148	T3719	T3718	prep	to,resistant
R2149	T3720	T3721	compound	copper,toxicity
R215	T450	T422	punct	.,expressed
R2150	T3721	T3719	pobj	toxicity,to
R2151	T3722	T3717	punct	.,confer
R2152	T3724	T3725	nsubj	Acdp,possess
R2153	T3726	T3725	dep	proteins,possess
R2154	T3727	T3725	advmod	also,possess
R2155	T3728	T3729	det	the,domains
R2156	T3729	T3725	dobj	domains,possess
R2157	T3730	T3731	dep	that,found
R2158	T3731	T3729	relcl	found,domains
R2159	T3732	T3731	auxpass	are,found
R216	T452	T453	nsubj	Immunostaining,revealed
R2160	T3733	T3731	prep	in,found
R2161	T3734	T3735	nmod	bacteria,CorC
R2162	T3735	T3736	nmod	CorC,proteins
R2163	T3736	T3733	pobj	proteins,in
R2164	T3737	T3735	cc	and,CorC
R2165	T3738	T3739	compound	yeast,Amip3
R2166	T3739	T3735	conj	Amip3,CorC
R2167	T3740	T3729	punct	", ",domains
R2168	T3741	T3742	amod	such,as
R2169	T3742	T3729	prep	as,domains
R217	T454	T452	prep	of,Immunostaining
R2170	T3743	T3744	det	the,domains
R2171	T3744	T3742	pobj	domains,as
R2172	T3745	T3744	compound	CBS,domains
R2173	T3746	T3744	punct	", ",domains
R2174	T3747	T3748	compound	DUF21,domain
R2175	T3748	T3744	conj	domain,domains
R2176	T3749	T3748	cc	and,domain
R2177	T3750	T3751	compound	transmembrane,domains
R2178	T3751	T3748	conj	domains,domain
R2179	T3752	T3725	punct	.,possess
R218	T455	T454	pobj	Acdp1,of
R2180	T3754	T3755	prep	In,found
R2181	T3756	T3754	pobj	addition,In
R2182	T3757	T3755	punct	", ",found
R2183	T3758	T3759	det	a,domain
R2184	T3759	T3755	nsubjpass	domain,found
R2185	T3760	T3761	npadvmod	cNMP,binding
R2186	T3761	T3759	amod	binding,domain
R2187	T3762	T3761	punct	-,binding
R2188	T3763	T3755	auxpass	was,found
R2189	T3764	T3755	prep	in,found
R219	T456	T455	prep	in,Acdp1
R2190	T3765	T3766	det	all,proteins
R2191	T3766	T3764	pobj	proteins,in
R2192	T3767	T3766	compound	Acdp,proteins
R2193	T3768	T3755	punct	", ",found
R2194	T3769	T3770	dep	which,is
R2195	T3770	T3755	ccomp	is,found
R2196	T3771	T3770	advmod	usually,is
R2197	T3772	T3770	acomp	present,is
R2198	T3773	T3770	prep	in,is
R2199	T3774	T3775	compound	ion,channels
R22	T243	T241	oprd	protein,named
R220	T457	T458	compound	hippocampus,neurons
R2200	T3775	T3773	pobj	channels,in
R2201	T3776	T3775	cc	and,channels
R2202	T3777	T3778	nmod	cNMP,kinases
R2203	T3778	T3775	conj	kinases,channels
R2204	T3779	T3777	punct	-,cNMP
R2205	T3780	T3777	amod	dependent,cNMP
R2206	T3781	T3782	punct	[,10
R2207	T3782	T3755	parataxis	10,found
R2208	T3783	T3784	punct	-,13
R2209	T3784	T3782	prep	13,10
R221	T458	T456	pobj	neurons,in
R2210	T3785	T3782	punct	],10
R2211	T3786	T3755	punct	.,found
R2212	T3788	T3789	advcl	Using,shown
R2213	T3790	T3788	dobj	antibody,Using
R2214	T3791	T3790	acl	produced,antibody
R2215	T3792	T3791	agent	by,produced
R2216	T3793	T3794	nmod	Acdp1,peptide
R2217	T3794	T3792	pobj	peptide,by
R2218	T3795	T3796	npadvmod	C,terminal
R2219	T3796	T3794	amod	terminal,peptide
R222	T459	T460	det	a,localization
R2220	T3797	T3796	punct	-,terminal
R2221	T3798	T3789	punct	", ",shown
R2222	T3799	T3789	nsubj	we,shown
R2223	T3800	T3789	aux	have,shown
R2224	T3801	T3802	mark	that,localized
R2225	T3802	T3789	ccomp	localized,shown
R2226	T3803	T3802	nsubjpass	Acdp1,localized
R2227	T3804	T3802	auxpass	is,localized
R2228	T3805	T3802	advmod	predominantly,localized
R2229	T3806	T3802	prep	on,localized
R223	T460	T453	dobj	localization,revealed
R2230	T3807	T3808	det	the,membrane
R2231	T3808	T3806	pobj	membrane,on
R2232	T3809	T3808	compound	plasma,membrane
R2233	T3810	T3802	prep	in,localized
R2234	T3811	T3812	compound	hippocampus,neurons
R2235	T3812	T3810	pobj	neurons,in
R2236	T3813	T3789	punct	.,shown
R2237	T3815	T3816	prep	In,found
R2238	T3817	T3818	poss	our,study
R2239	T3818	T3815	pobj	study,In
R224	T461	T460	amod	predominant,localization
R2240	T3819	T3818	amod	previous,study
R2241	T3820	T3816	punct	", ",found
R2242	T3821	T3816	nsubj	we,found
R2243	T3822	T3823	amod	human,proteins
R2244	T3823	T3825	nsubjpass	proteins,localized
R2245	T3824	T3823	compound	ACDP,proteins
R2246	T3825	T3816	advcl	localized,found
R2247	T3826	T3825	auxpass	are,localized
R2248	T3827	T3825	advmod	predominantly,localized
R2249	T3828	T3825	prep	in,localized
R225	T462	T453	prep	on,revealed
R2250	T3829	T3830	det	the,nucleus
R2251	T3830	T3828	pobj	nucleus,in
R2252	T3831	T3825	prep	in,localized
R2253	T3832	T3833	compound	HeLa,cells
R2254	T3833	T3831	pobj	cells,in
R2255	T3834	T3835	punct	[,1
R2256	T3835	T3816	parataxis	1,found
R2257	T3836	T3835	punct	],1
R2258	T3837	T3816	punct	.,found
R2259	T3839	T3840	det	The,discrepancy
R226	T463	T464	det	the,membrane
R2260	T3840	T3841	nsubjpass	discrepancy,caused
R2261	T3842	T3840	prep	for,discrepancy
R2262	T3843	T3844	compound	Acdp,localization
R2263	T3844	T3842	pobj	localization,for
R2264	T3845	T3844	prep	in,localization
R2265	T3846	T3847	amod	neuronal,cells
R2266	T3847	T3845	pobj	cells,in
R2267	T3848	T3841	aux	could,caused
R2268	T3849	T3841	auxpass	be,caused
R2269	T3850	T3841	agent	by,caused
R227	T464	T462	pobj	membrane,on
R2270	T3851	T3850	pobj	non-specificity,by
R2271	T3852	T3851	prep	for,non-specificity
R2272	T3853	T3854	amod	previous,antibody
R2273	T3854	T3852	pobj	antibody,for
R2274	T3855	T3856	dep	which,produced
R2275	T3856	T3854	relcl	produced,antibody
R2276	T3857	T3856	auxpass	was,produced
R2277	T3858	T3856	prep	by,produced
R2278	T3859	T3860	det	the,domain
R2279	T3860	T3858	pobj	domain,by
R228	T465	T464	compound	plasma,membrane
R2280	T3861	T3860	amod	recombinant,domain
R2281	T3862	T3860	compound	ACD,domain
R2282	T3863	T3851	punct	", ",non-specificity
R2283	T3864	T3851	cc	or,non-specificity
R2284	T3865	T3866	amod	different,cells
R2285	T3866	T3851	conj	cells,non-specificity
R2286	T3867	T3866	acl	used,cells
R2287	T3868	T3841	punct	.,caused
R2288	T3870	T3871	prep	In,recognizes
R2289	T3872	T3873	amod	current,study
R229	T466	T453	punct	.,revealed
R2290	T3873	T3870	pobj	study,In
R2291	T3874	T3871	punct	", ",recognizes
R2292	T3875	T3876	det	the,antibody
R2293	T3876	T3871	nsubj	antibody,recognizes
R2294	T3877	T3876	compound	Acdp1,antibody
R2295	T3878	T3879	compound	C,terminus
R2296	T3879	T3876	compound	terminus,antibody
R2297	T3880	T3879	punct	-,terminus
R2298	T3881	T3871	advmod	only,recognizes
R2299	T3882	T3871	dobj	Acdp1,recognizes
R23	T244	T243	amod	conserved,protein
R230	T470	T471	det	The,genes
R2300	T3883	T3871	prep	in,recognizes
R2301	T3884	T3885	compound	brain,tissue
R2302	T3885	T3886	compound	tissue,extracts
R2303	T3886	T3883	pobj	extracts,in
R2304	T3887	T3888	advmod	as,as
R2305	T3888	T3883	cc	as,in
R2306	T3889	T3888	advmod	well,as
R2307	T3890	T3883	conj	in,in
R2308	T3891	T3892	nmod	HEK293,lysates
R2309	T3892	T3890	pobj	lysates,in
R231	T471	T473	nsubjpass	genes,conserved
R2310	T3893	T3891	punct	", ",HEK293
R2311	T3894	T3891	conj	3T3,HEK293
R2312	T3895	T3894	cc	and,3T3
R2313	T3896	T3894	conj	PC12,3T3
R2314	T3897	T3892	compound	cell,lysates
R2315	T3898	T3871	punct	", ",recognizes
R2316	T3899	T3871	advcl	suggesting,recognizes
R2317	T3900	T3901	det	the,specificity
R2318	T3901	T3899	dobj	specificity,suggesting
R2319	T3902	T3901	prep	of,specificity
R232	T472	T471	compound	Acdp,genes
R2320	T3903	T3904	det	the,antibody
R2321	T3904	T3902	pobj	antibody,of
R2322	T3905	T3871	punct	.,recognizes
R2323	T3907	T3908	poss	Our,observations
R2324	T3908	T3909	nsubj	observations,suggested
R2325	T3910	T3911	mark	that,involved
R2326	T3911	T3909	ccomp	involved,suggested
R2327	T3912	T3911	nsubjpass	Acdp,involved
R2328	T3913	T3911	aux	might,involved
R2329	T3914	T3911	auxpass	be,involved
R233	T474	T473	auxpass	are,conserved
R2330	T3915	T3911	prep	in,involved
R2331	T3916	T3917	compound	ion,transport
R2332	T3917	T3915	pobj	transport,in
R2333	T3918	T3911	prep	in,involved
R2334	T3919	T3920	amod	mammalian,cells
R2335	T3920	T3918	pobj	cells,in
R2336	T3921	T3909	punct	.,suggested
R2337	T3923	T3924	advmod	However,needed
R2338	T3925	T3924	punct	", ",needed
R2339	T3926	T3927	advmod	more,detailed
R234	T475	T473	advmod	evolutionarily,conserved
R2340	T3927	T3928	amod	detailed,studies
R2341	T3928	T3924	nsubjpass	studies,needed
R2342	T3929	T3928	amod	functional,studies
R2343	T3930	T3924	auxpass	are,needed
R2344	T3931	T3932	aux	to,demonstrate
R2345	T3932	T3924	advcl	demonstrate,needed
R2346	T3933	T3934	det	the,functions
R2347	T3934	T3932	dobj	functions,demonstrate
R2348	T3935	T3934	amod	real,functions
R2349	T3936	T3934	prep	of,functions
R235	T476	T473	prep	in,conserved
R2350	T3937	T3938	det	these,proteins
R2351	T3938	T3936	pobj	proteins,of
R2352	T3939	T3924	punct	.,needed
R2353	T4051	T4052	poss	Our,work
R2354	T4052	T4054	nsubj	work,described
R2355	T4053	T4052	amod	previous,work
R2356	T4055	T4054	aux	has,described
R2357	T4056	T4057	amod	human,genes
R2358	T4057	T4054	dobj	genes,described
R2359	T4058	T4057	compound	ACDP,genes
R236	T477	T478	amod	diverse,species
R2360	T4059	T4060	punct	[,1
R2361	T4060	T4054	parataxis	1,described
R2362	T4061	T4060	punct	],1
R2363	T4062	T4054	punct	.,described
R2364	T4064	T4065	det	The,information
R2365	T4065	T4067	nsubj	information,includes
R2366	T4066	T4065	amod	new,information
R2367	T4067	T4072	ccomp	includes,is
R2368	T4068	T4065	prep	of,information
R2369	T4069	T4070	det	the,work
R237	T478	T476	pobj	species,in
R2370	T4070	T4068	pobj	work,of
R2371	T4071	T4070	amod	current,work
R2372	T4073	T4067	punct	: ,includes
R2373	T4074	T4072	meta	1,is
R2374	T4075	T4074	punct	),1
R2375	T4076	T4074	punct	.,1
R2376	T4077	T4072	nsubj	It,is
R2377	T4078	T4079	det	the,time
R2378	T4079	T4072	attr	time,is
R2379	T4080	T4079	amod	first,time
R238	T479	T473	cc	and,conserved
R2380	T4081	T4082	aux	to,report
R2381	T4082	T4079	advcl	report,time
R2382	T4083	T4084	det	the,existence
R2383	T4084	T4082	dobj	existence,report
R2384	T4085	T4084	prep	of,existence
R2385	T4086	T4087	amod	multiple,genes
R2386	T4087	T4085	pobj	genes,of
R2387	T4088	T4087	compound	Acdp,genes
R2388	T4089	T4082	prep	in,report
R2389	T4090	T4091	amod	other,mammals
R239	T480	T481	advmod	ubiquitously,expressed
R2390	T4091	T4089	pobj	mammals,in
R2391	T4092	T4091	prep	in,mammals
R2392	T4093	T4092	pobj	addition,in
R2393	T4094	T4093	prep	to,addition
R2394	T4095	T4094	pobj	human,to
R2395	T4096	T4072	punct	", ",is
R2396	T4097	T4098	mark	while,appears
R2397	T4098	T4072	advcl	appears,is
R2398	T4099	T4098	nsubj	Acdp,appears
R2399	T4100	T4101	aux	to,be
R24	T245	T243	compound	domain,protein
R240	T481	T473	conj	expressed,conserved
R2400	T4101	T4098	xcomp	be,appears
R2401	T4102	T4103	det	a,gene
R2402	T4103	T4101	attr	gene,be
R2403	T4104	T4103	amod	single,gene
R2404	T4105	T4103	compound	copy,gene
R2405	T4106	T4103	prep	in,gene
R2406	T4107	T4108	amod	lower,organisms
R2407	T4108	T4106	pobj	organisms,in
R2408	T4109	T4110	amod	such,as
R2409	T4110	T4101	prep	as,be
R241	T482	T481	prep	throughout,expressed
R2410	T4111	T4110	prep	in,as
R2411	T4112	T4113	compound	C.,elegans
R2412	T4113	T4111	pobj	elegans,in
R2413	T4114	T4113	punct	", ",elegans
R2414	T4115	T4113	conj	yeasts,elegans
R2415	T4116	T4115	cc	and,yeasts
R2416	T4117	T4115	conj	bacteria,yeasts
R2417	T4118	T4072	punct	.,is
R2418	T4120	T4121	nsubj	We,suggested
R2419	T4121	T4124	ccomp	suggested,generated
R242	T483	T482	pobj	development,throughout
R2420	T4122	T4121	aux	have,suggested
R2421	T4123	T4121	advmod	also,suggested
R2422	T4125	T4126	det	the,relationships
R2423	T4126	T4121	dobj	relationships,suggested
R2424	T4127	T4126	amod	evolutionary,relationships
R2425	T4128	T4126	prep	of,relationships
R2426	T4129	T4130	compound	Acdp,genes
R2427	T4130	T4128	pobj	genes,of
R2428	T4131	T4130	prep	in,genes
R2429	T4132	T4133	amod	different,species
R243	T484	T483	cc	and,development
R2430	T4133	T4131	pobj	species,in
R2431	T4134	T4135	punct	(,tree
R2432	T4135	T4121	parataxis	tree,suggested
R2433	T4136	T4135	amod	phylogenetic,tree
R2434	T4137	T4135	punct	),tree
R2435	T4138	T4124	punct	;,generated
R2436	T4139	T4140	meta	2,are
R2437	T4140	T4124	ccomp	are,generated
R2438	T4141	T4139	punct	),2
R2439	T4142	T4139	punct	.,2
R244	T485	T486	amod	adult,tissues
R2440	T4143	T4144	amod	Molecular,cloning
R2441	T4144	T4140	nsubj	cloning,are
R2442	T4145	T4144	cc	and,cloning
R2443	T4146	T4144	conj	characterization,cloning
R2444	T4147	T4144	prep	of,cloning
R2445	T4148	T4149	amod	murine,family
R2446	T4149	T4147	pobj	family,of
R2447	T4150	T4149	compound	Acdp,family
R2448	T4151	T4149	compound	gene,family
R2449	T4152	T4140	acomp	essential,are
R245	T486	T483	conj	tissues,development
R2450	T4153	T4140	prep	for,are
R2451	T4154	T4153	pobj	study,for
R2452	T4155	T4154	prep	of,study
R2453	T4156	T4157	det	this,family
R2454	T4157	T4155	pobj	family,of
R2455	T4158	T4157	amod	novel,family
R2456	T4159	T4157	compound	gene,family
R2457	T4160	T4154	prep	in,study
R2458	T4161	T4162	compound	animal,model
R2459	T4162	T4160	pobj	model,in
R246	T487	T473	advcl	suggesting,conserved
R2460	T4163	T4153	punct	", ",for
R2461	T4164	T4165	advmod	e.g.,for
R2462	T4165	T4153	prep	for,for
R2463	T4166	T4165	pobj	generation,for
R2464	T4167	T4166	prep	of,generation
R2465	T4168	T4169	nmod	knockout,models
R2466	T4169	T4167	pobj	models,of
R2467	T4170	T4168	cc	or,knockout
R2468	T4171	T4168	conj	transgenic,knockout
R2469	T4172	T4124	punct	;,generated
R247	T488	T489	mark	that,be
R2470	T4173	T4174	meta	3,demonstrated
R2471	T4174	T4124	ccomp	demonstrated,generated
R2472	T4175	T4173	punct	),3
R2473	T4176	T4173	punct	.,3
R2474	T4177	T4174	nsubj	We,demonstrated
R2475	T4178	T4174	aux	have,demonstrated
R2476	T4179	T4180	preconj	both,DNA
R2477	T4180	T4181	nmod	DNA,conservations
R2478	T4181	T4174	dobj	conservations,demonstrated
R2479	T4182	T4180	cc	and,DNA
R248	T489	T487	ccomp	be,suggesting
R2480	T4183	T4184	compound	amino,acid
R2481	T4184	T4180	conj	acid,DNA
R2482	T4185	T4181	prep	between,conservations
R2483	T4186	T4185	pobj	mouse,between
R2484	T4187	T4186	cc	and,mouse
R2485	T4188	T4186	conj	human,mouse
R2486	T4189	T4174	prep	for,demonstrated
R2487	T4190	T4191	det	each,gene
R2488	T4191	T4189	pobj	gene,for
R2489	T4192	T4191	compound	Acdp,gene
R249	T490	T489	nsubj	Acdp,be
R2490	T4193	T4191	cc	and,gene
R2491	T4194	T4195	det	the,family
R2492	T4195	T4191	conj	family,gene
R2493	T4196	T4195	amod	whole,family
R2494	T4197	T4195	compound	Acdp,family
R2495	T4198	T4195	compound	gene,family
R2496	T4199	T4191	punct	", ",gene
R2497	T4200	T4201	dep	which,provide
R2498	T4201	T4191	relcl	provide,gene
R2499	T4202	T4203	amod	important,information
R25	T246	T243	punct	(,protein
R250	T491	T489	aux	may,be
R2500	T4203	T4201	dobj	information,provide
R2501	T4204	T4203	prep	for,information
R2502	T4205	T4206	det	the,possibility
R2503	T4206	T4204	pobj	possibility,for
R2504	T4207	T4206	prep	of,possibility
R2505	T4208	T4209	amod	functional,redundancy
R2506	T4209	T4207	pobj	redundancy,of
R2507	T4210	T4209	cc	or,redundancy
R2508	T4211	T4209	conj	overlap,redundancy
R2509	T4212	T4209	prep	between,redundancy
R251	T492	T493	det	an,gene
R2510	T4213	T4214	compound	Acdp,members
R2511	T4214	T4212	pobj	members,between
R2512	T4215	T4124	punct	;,generated
R2513	T4216	T4124	meta	4,generated
R2514	T4217	T4216	punct	),4
R2515	T4218	T4216	punct	.,4
R2516	T4219	T4124	nsubj	We,generated
R2517	T4220	T4124	aux	have,generated
R2518	T4221	T4124	dobj	antibodies,generated
R2519	T4222	T4221	amod	specific,antibodies
R252	T493	T489	attr	gene,be
R2520	T4223	T4222	prep	for,specific
R2521	T4224	T4223	pobj	Acdp1,for
R2522	T4225	T4224	cc	and,Acdp1
R2523	T4226	T4227	det	all,proteins
R2524	T4227	T4224	conj	proteins,Acdp1
R2525	T4228	T4227	compound	Acdp,proteins
R2526	T4229	T4124	punct	.,generated
R2527	T4231	T4232	det	The,antibody
R2528	T4232	T4237	nsubj	antibody,appears
R2529	T4233	T4232	compound	Acdp1,antibody
R253	T494	T493	amod	essential,gene
R2530	T4234	T4235	compound	C,terminus
R2531	T4235	T4232	compound	terminus,antibody
R2532	T4236	T4235	punct	-,terminus
R2533	T4238	T4239	aux	to,recognize
R2534	T4239	T4237	xcomp	recognize,appears
R2535	T4240	T4239	advmod	specifically,recognize
R2536	T4241	T4239	dobj	Acdp1,recognize
R2537	T4242	T4237	punct	.,appears
R2538	T4244	T4245	advcl	Using,demonstrated
R2539	T4246	T4247	det	this,antibody
R254	T495	T473	punct	.,conserved
R2540	T4247	T4244	dobj	antibody,Using
R2541	T4248	T4245	punct	", ",demonstrated
R2542	T4249	T4245	nsubj	we,demonstrated
R2543	T4250	T4245	aux	have,demonstrated
R2544	T4251	T4252	mark	that,localized
R2545	T4252	T4245	ccomp	localized,demonstrated
R2546	T4253	T4252	nsubjpass	Acdp1,localized
R2547	T4254	T4252	auxpass	is,localized
R2548	T4255	T4252	advmod	predominantly,localized
R2549	T4256	T4252	prep	on,localized
R255	T497	T498	nsubj	Acdp,showed
R2550	T4257	T4258	det	the,membrane
R2551	T4258	T4256	pobj	membrane,on
R2552	T4259	T4258	compound	plasma,membrane
R2553	T4260	T4252	prep	in,localized
R2554	T4261	T4262	compound	hippocampus,neurons
R2555	T4262	T4260	pobj	neurons,in
R2556	T4263	T4245	punct	.,demonstrated
R2557	T4265	T4266	det	These,results
R2558	T4266	T4267	nsubj	results,represent
R2559	T4268	T4269	det	an,step
R256	T499	T500	amod	strong,homology
R2560	T4269	T4267	dobj	step,represent
R2561	T4270	T4269	amod	important,step
R2562	T4271	T4269	prep	towards,step
R2563	T4272	T4273	det	the,characterization
R2564	T4273	T4271	pobj	characterization,towards
R2565	T4274	T4273	prep	of,characterization
R2566	T4275	T4274	pobj	functions,of
R2567	T4276	T4273	prep	for,characterization
R2568	T4277	T4278	det	the,family
R2569	T4278	T4276	pobj	family,for
R257	T500	T498	dobj	homology,showed
R2570	T4279	T4278	compound	Acdp,family
R2571	T4280	T4278	compound	gene,family
R2572	T4281	T4267	punct	.,represent
R2573	T4392	T4393	compound	cDNA,cloning
R2574	T4395	T4396	compound	cDNA,cloning
R2575	T4396	T4397	nsubjpass	cloning,performed
R2576	T4398	T4396	prep	of,cloning
R2577	T4399	T4400	det	the,family
R2578	T4400	T4398	pobj	family,of
R2579	T4401	T4400	compound	Acdp,family
R258	T501	T498	prep	to,showed
R2580	T4402	T4400	compound	gene,family
R2581	T4403	T4397	auxpass	was,performed
R2582	T4404	T4397	prep	based,performed
R2583	T4405	T4404	prep	on,based
R2584	T4406	T4407	amod	human,sequences
R2585	T4407	T4405	pobj	sequences,on
R2586	T4408	T4407	compound	homologue,sequences
R2587	T4409	T4410	mark	as,reported
R2588	T4410	T4397	advcl	reported,performed
R2589	T4411	T4410	advmod	previously,reported
R259	T502	T503	compound	bacteria,protein
R2590	T4412	T4413	punct	[,2
R2591	T4413	T4397	parataxis	2,performed
R2592	T4414	T4413	punct	],2
R2593	T4415	T4397	punct	.,performed
R2594	T4417	T4418	advmod	Briefly,used
R2595	T4419	T4418	punct	", ",used
R2596	T4420	T4421	det	the,cDNAs
R2597	T4421	T4418	nsubjpass	cDNAs,used
R2598	T4422	T4421	amod	human,cDNAs
R2599	T4423	T4421	compound	ACDP,cDNAs
R26	T247	T243	appos	ACDP,protein
R260	T503	T501	pobj	protein,to
R2600	T4424	T4421	cc	and,cDNAs
R2601	T4425	T4426	amod	predicted,sequences
R2602	T4426	T4421	conj	sequences,cDNAs
R2603	T4427	T4426	compound	AA,sequences
R2604	T4428	T4418	auxpass	were,used
R2605	T4429	T4430	aux	to,search
R2606	T4430	T4418	advcl	search,used
R2607	T4431	T4432	det	the,database
R2608	T4432	T4430	dobj	database,search
R2609	T4433	T4432	compound	mouse,database
R261	T504	T503	compound	CorC,protein
R2610	T4434	T4432	compound	EST,database
R2611	T4435	T4430	prep	for,search
R2612	T4436	T4437	compound	EST,markers
R2613	T4437	T4435	pobj	markers,for
R2614	T4438	T4437	acl	corresponding,markers
R2615	T4439	T4438	prep	to,corresponding
R2616	T4440	T4441	det	each,member
R2617	T4441	T4439	pobj	member,to
R2618	T4442	T4441	compound	Acdp,member
R2619	T4443	T4418	punct	.,used
R262	T505	T498	cc	and,showed
R2620	T4445	T4446	det	A,primer
R2621	T4446	T4448	nsubjpass	primer,used
R2622	T4447	T4446	amod	forward,primer
R2623	T4449	T4446	prep	within,primer
R2624	T4450	T4451	det	the,region
R2625	T4451	T4449	pobj	region,within
R2626	T4452	T4451	amod	human,region
R2627	T4453	T4451	nmod	ACDP,region
R2628	T4454	T4451	nummod	5,region
R2629	T4455	T4454	punct	',5
R263	T506	T507	advmod	predominantly,localized
R2630	T4456	T4451	compound	cDNA,region
R2631	T4457	T4451	compound	coding,region
R2632	T4458	T4446	punct	(,primer
R2633	T4459	T4446	prep	after,primer
R2634	T4460	T4461	compound	start,codon
R2635	T4461	T4459	pobj	codon,after
R2636	T4462	T4446	punct	),primer
R2637	T4463	T4446	cc	and,primer
R2638	T4464	T4465	det	a,primer
R2639	T4465	T4446	conj	primer,primer
R264	T507	T498	conj	localized,showed
R2640	T4466	T4465	nmod	mouse,primer
R2641	T4467	T4465	amod	reverse,primer
R2642	T4468	T4465	prep	from,primer
R2643	T4469	T4470	det	the,marker
R2644	T4470	T4468	pobj	marker,from
R2645	T4471	T4470	compound	mouse,marker
R2646	T4472	T4470	compound	EST,marker
R2647	T4473	T4448	auxpass	were,used
R2648	T4474	T4475	aux	to,amplify
R2649	T4475	T4448	advcl	amplify,used
R265	T508	T507	prep	on,localized
R2650	T4476	T4477	det	the,sequence
R2651	T4477	T4475	dobj	sequence,amplify
R2652	T4478	T4477	compound	homologue,sequence
R2653	T4479	T4475	prep	from,amplify
R2654	T4480	T4481	compound	mouse,cDNA
R2655	T4481	T4479	pobj	cDNA,from
R2656	T4482	T4475	prep	at,amplify
R2657	T4483	T4484	advmod	very,low
R2658	T4484	T4485	amod	low,temperature
R2659	T4485	T4482	pobj	temperature,at
R266	T509	T510	det	the,membrane
R2660	T4486	T4485	amod	annealing,temperature
R2661	T4487	T4485	punct	(,temperature
R2662	T4488	T4489	quantmod	45,50
R2663	T4489	T4491	nummod	50,°C
R2664	T4490	T4489	punct	–,50
R2665	T4491	T4485	appos	°C,temperature
R2666	T4492	T4448	punct	),used
R2667	T4493	T4448	punct	.,used
R2668	T4495	T4496	det	A,PCR
R2669	T4496	T4498	nsubjpass	PCR,carried
R267	T510	T508	pobj	membrane,on
R2670	T4497	T4496	amod	nested,PCR
R2671	T4499	T4496	acl	using,PCR
R2672	T4500	T4501	det	an,primer
R2673	T4501	T4499	dobj	primer,using
R2674	T4502	T4501	amod	inside,primer
R2675	T4503	T4501	amod	reverse,primer
R2676	T4504	T4501	prep	from,primer
R2677	T4505	T4506	det	the,sequence
R2678	T4506	T4504	pobj	sequence,from
R2679	T4507	T4506	compound	mouse,sequence
R268	T511	T510	compound	plasma,membrane
R2680	T4508	T4506	compound	EST,sequence
R2681	T4509	T4501	cc	and,primer
R2682	T4510	T4511	det	the,primer
R2683	T4511	T4501	conj	primer,primer
R2684	T4512	T4511	amod	same,primer
R2685	T4513	T4511	amod	human,primer
R2686	T4514	T4511	amod	forward,primer
R2687	T4515	T4498	auxpass	was,carried
R2688	T4516	T4498	advmod	then,carried
R2689	T4517	T4498	prt	out,carried
R269	T512	T498	punct	.,showed
R2690	T4518	T4519	aux	to,amplify
R2691	T4519	T4498	advcl	amplify,carried
R2692	T4520	T4521	det	the,gene
R2693	T4521	T4519	dobj	gene,amplify
R2694	T4522	T4521	amod	specific,gene
R2695	T4523	T4521	compound	mouse,gene
R2696	T4524	T4521	prep	from,gene
R2697	T4525	T4526	det	the,products
R2698	T4526	T4524	pobj	products,from
R2699	T4527	T4528	amod	first,round
R27	T248	T241	punct	),named
R270	T514	T515	det	These,results
R2700	T4528	T4526	compound	round,products
R2701	T4529	T4526	compound	PCR,products
R2702	T4530	T4519	prep	at,amplify
R2703	T4531	T4532	amod	high,temperature
R2704	T4532	T4530	pobj	temperature,at
R2705	T4533	T4532	amod	annealing,temperature
R2706	T4534	T4532	punct	(,temperature
R2707	T4535	T4536	nummod	62,°C
R2708	T4536	T4532	appos	°C,temperature
R2709	T4537	T4498	punct	),carried
R271	T515	T516	nsubj	results,suggest
R2710	T4538	T4498	punct	.,carried
R2711	T4540	T4541	det	The,products
R2712	T4541	T4544	nsubjpass	products,excised
R2713	T4542	T4541	amod	expected,products
R2714	T4543	T4541	compound	PCR,products
R2715	T4545	T4544	auxpass	were,excised
R2716	T4546	T4544	advmod	directly,excised
R2717	T4547	T4544	prep	from,excised
R2718	T4548	T4549	compound	agarose,gel
R2719	T4549	T4547	pobj	gel,from
R272	T517	T518	mark	that,is
R2720	T4550	T4544	cc	and,excised
R2721	T4551	T4544	conj	sequenced,excised
R2722	T4552	T4551	prep	by,sequenced
R2723	T4553	T4554	det	an,sequencer
R2724	T4554	T4552	pobj	sequencer,by
R2725	T4555	T4554	nmod	ABI377,sequencer
R2726	T4556	T4554	amod	automatic,sequencer
R2727	T4557	T4544	punct	.,excised
R2728	T4559	T4560	det	The,sequence
R2729	T4560	T4561	nsubjpass	sequence,confirmed
R273	T518	T516	ccomp	is,suggest
R2730	T4562	T4561	auxpass	was,confirmed
R2731	T4563	T4561	advmod	further,confirmed
R2732	T4564	T4561	advcl	using,confirmed
R2733	T4565	T4566	det	a,primer
R2734	T4566	T4564	dobj	primer,using
R2735	T4567	T4566	amod	forward,primer
R2736	T4568	T4566	prep	from,primer
R2737	T4569	T4570	advmod	newly,identified
R2738	T4570	T4571	amod	identified,sequence
R2739	T4571	T4568	pobj	sequence,from
R274	T519	T518	nsubj	Acdp,is
R2740	T4572	T4566	cc	and,primer
R2741	T4573	T4574	det	a,primer
R2742	T4574	T4566	conj	primer,primer
R2743	T4575	T4574	amod	reverse,primer
R2744	T4576	T4574	prep	from,primer
R2745	T4577	T4578	amod	known,sequence
R2746	T4578	T4576	pobj	sequence,from
R2747	T4579	T4561	punct	.,confirmed
R2748	T4581	T4582	mark	Once,identified
R2749	T4582	T4589	advcl	identified,obtained
R275	T520	T518	advmod	probably,is
R2750	T4583	T4582	nsubjpass	most,identified
R2751	T4584	T4583	prep	of,most
R2752	T4585	T4586	det	the,sequences
R2753	T4586	T4584	pobj	sequences,of
R2754	T4587	T4586	amod	coding,sequences
R2755	T4588	T4582	auxpass	were,identified
R2756	T4590	T4589	punct	", ",obtained
R2757	T4591	T4592	amod	partial,sequence
R2758	T4592	T4589	nsubjpass	sequence,obtained
R2759	T4593	T4592	prep	of,sequence
R276	T521	T522	det	a,family
R2760	T4594	T4595	nmod	exon,sequences
R2761	T4595	T4593	pobj	sequences,of
R2762	T4596	T4594	nummod	1,exon
R2763	T4597	T4594	cc	and,exon
R2764	T4598	T4599	det	the,UTR
R2765	T4599	T4594	conj	UTR,exon
R2766	T4600	T4599	nummod	5,UTR
R2767	T4601	T4600	punct	',5
R2768	T4602	T4589	auxpass	were,obtained
R2769	T4603	T4589	prep	by,obtained
R277	T522	T518	attr	family,is
R2770	T4604	T4605	compound	BAC,sequencing
R2771	T4605	T4603	pobj	sequencing,by
R2772	T4606	T4605	compound	DNA,sequencing
R2773	T4607	T4589	punct	.,obtained
R2774	T4647	T4648	compound	Northern,blot
R2775	T4648	T4649	compound	blot,analyses
R2776	T4651	T4652	amod	Multiple,filters
R2777	T4652	T4656	nsubjpass	filters,purchased
R2778	T4653	T4652	compound	Choice,filters
R2779	T4654	T4655	compound	Northern,Blot
R278	T523	T522	prep	of,family
R2780	T4655	T4652	compound	Blot,filters
R2781	T4657	T4652	acl	containing,filters
R2782	T4658	T4659	nummod	12,tissues
R2783	T4659	T4657	dobj	tissues,containing
R2784	T4660	T4659	amod	different,tissues
R2785	T4661	T4659	compound	mouse,tissues
R2786	T4662	T4656	auxpass	were,purchased
R2787	T4663	T4656	prep	from,purchased
R2788	T4664	T4663	pobj	Origene,from
R2789	T4665	T4656	punct	.,purchased
R279	T524	T523	pobj	proteins,of
R2790	T4667	T4668	det	The,filters
R2791	T4668	T4669	nsubjpass	filters,probed
R2792	T4670	T4669	auxpass	were,probed
R2793	T4671	T4669	prep	for,probed
R2794	T4672	T4673	det	each,gene
R2795	T4673	T4671	pobj	gene,for
R2796	T4674	T4673	compound	Acdp,gene
R2797	T4675	T4669	prep	with,probed
R2798	T4676	T4677	det	a,fragment
R2799	T4677	T4675	pobj	fragment,with
R28	T249	T236	prep	in,characterized
R280	T525	T524	acl	involved,proteins
R2800	T4678	T4677	compound	PCR,fragment
R2801	T4679	T4680	punct	(,bp
R2802	T4680	T4677	parataxis	bp,fragment
R2803	T4681	T4682	quantmod	around,350
R2804	T4682	T4680	nummod	350,bp
R2805	T4683	T4680	punct	),bp
R2806	T4684	T4677	prep	from,fragment
R2807	T4685	T4686	amod	last,exon
R2808	T4686	T4684	pobj	exon,from
R2809	T4687	T4669	cc	and,probed
R281	T526	T525	prep	in,involved
R2810	T4688	T4689	det	the,3
R2811	T4689	T4690	nummod	3,region
R2812	T4690	T4693	nsubj	region,labeled
R2813	T4691	T4689	punct	',3
R2814	T4692	T4689	amod	untranslated,3
R2815	T4693	T4669	conj	labeled,probed
R2816	T4694	T4693	prep	with,labeled
R2817	T4695	T4696	compound	α,32P
R2818	T4696	T4698	compound	32P,dCTP
R2819	T4697	T4696	punct	-,32P
R282	T527	T528	compound	ion,transport
R2820	T4698	T4694	pobj	dCTP,with
R2821	T4699	T4693	advcl	using,labeled
R2822	T4700	T4701	det	the,system
R2823	T4701	T4699	dobj	system,using
R2824	T4702	T4701	amod	random,system
R2825	T4703	T4701	compound	primer,system
R2826	T4704	T4701	compound	extension,system
R2827	T4705	T4706	punct	(,Technologies
R2828	T4706	T4701	parataxis	Technologies,system
R2829	T4707	T4706	compound	Life,Technologies
R283	T528	T526	pobj	transport,in
R2830	T4708	T4706	punct	),Technologies
R2831	T4709	T4693	punct	.,labeled
R2832	T4711	T4712	nsubjpass	Hybridizations,carried
R2833	T4713	T4712	auxpass	were,carried
R2834	T4714	T4712	prt	out,carried
R2835	T4715	T4712	advmod	overnight,carried
R2836	T4716	T4712	punct	.,carried
R2837	T4718	T4719	det	The,filters
R2838	T4719	T4720	nsubjpass	filters,washed
R2839	T4721	T4720	auxpass	were,washed
R284	T529	T525	prep	in,involved
R2840	T4722	T4720	advmod	twice,washed
R2841	T4723	T4720	prep	with,washed
R2842	T4724	T4725	amod	washing,buffer
R2843	T4725	T4723	pobj	buffer,with
R2844	T4726	T4725	nummod	I,buffer
R2845	T4727	T4728	punct	(,SDS
R2846	T4728	T4725	parataxis	SDS,buffer
R2847	T4729	T4730	nummod	2,SSC
R2848	T4730	T4728	dep	SSC,SDS
R2849	T4731	T4730	punct	×,SSC
R285	T530	T531	amod	mammalian,cells
R2850	T4732	T4728	punct	", ",SDS
R2851	T4733	T4734	nummod	0.1,%
R2852	T4734	T4728	compound	%,SDS
R2853	T4735	T4728	punct	),SDS
R2854	T4736	T4720	prep	at,washed
R2855	T4737	T4738	nummod	42,°C
R2856	T4738	T4736	pobj	°C,at
R2857	T4739	T4720	prep	for,washed
R2858	T4740	T4741	nummod	15,min
R2859	T4741	T4739	pobj	min,for
R286	T531	T529	pobj	cells,in
R2860	T4742	T4741	punct	-,min
R2861	T4743	T4720	punct	", ",washed
R2862	T4744	T4720	cc	and,washed
R2863	T4745	T4746	advmod	then,washed
R2864	T4746	T4720	conj	washed,washed
R2865	T4747	T4746	advmod	twice,washed
R2866	T4748	T4746	prep	with,washed
R2867	T4749	T4750	compound	washing,buffer
R2868	T4750	T4748	pobj	buffer,with
R2869	T4751	T4750	nummod	II,buffer
R287	T216	T217	amod	Molecular,cloning
R2870	T4752	T4753	punct	(,SDS
R2871	T4753	T4750	parataxis	SDS,buffer
R2872	T4754	T4755	nummod	0.25,SSC
R2873	T4755	T4753	dep	SSC,SDS
R2874	T4756	T4755	punct	×,SSC
R2875	T4757	T4753	punct	", ",SDS
R2876	T4758	T4759	nummod	0.1,%
R2877	T4759	T4753	compound	%,SDS
R2878	T4760	T4753	punct	),SDS
R2879	T4761	T4746	prep	at,washed
R288	T593	T594	nsubj	We,cloned
R2880	T4762	T4763	nummod	65,°C
R2881	T4763	T4761	pobj	°C,at
R2882	T4764	T4746	prep	for,washed
R2883	T4765	T4766	nummod	15,min
R2884	T4766	T4764	pobj	min,for
R2885	T4767	T4766	punct	-,min
R2886	T4768	T4720	punct	.,washed
R2887	T4770	T4771	amod	Washed,filters
R2888	T4771	T4772	nsubjpass	filters,exposed
R2889	T4773	T4772	auxpass	were,exposed
R289	T595	T594	aux	have,cloned
R2890	T4774	T4772	prep	to,exposed
R2891	T4775	T4776	compound	X-ray,films
R2892	T4776	T4774	pobj	films,to
R2893	T4777	T4772	prep	for,exposed
R2894	T4778	T4777	pcomp	overnight,for
R2895	T4779	T4778	cc	or,overnight
R2896	T4780	T4778	conj	longer,overnight
R2897	T4781	T4782	punct	[,4
R2898	T4782	T4772	parataxis	4,exposed
R2899	T4783	T4782	nummod	3,4
R29	T250	T249	pobj	humans,in
R290	T596	T594	advmod	recently,cloned
R2900	T4784	T4782	punct	",",4
R2901	T4785	T4782	punct	],4
R2902	T4786	T4772	punct	.,exposed
R2904	T4823	T4824	compound	Antibody,production
R2905	T4825	T4824	cc	and,production
R2906	T4826	T4827	compound	Western,blot
R2907	T4827	T4828	compound	blot,analyses
R2908	T4828	T4824	conj	analyses,production
R2909	T4830	T4831	nsubjpass	Peptides,used
R291	T597	T594	cc	and,cloned
R2910	T4832	T4830	acl	linked,Peptides
R2911	T4833	T4832	prep	to,linked
R2912	T4834	T4833	pobj	KLH,to
R2913	T4835	T4834	punct	(,KLH
R2914	T4836	T4837	compound	keyhole,hemacyanin
R2915	T4837	T4834	appos	hemacyanin,KLH
R2916	T4838	T4837	compound	limpet,hemacyanin
R2917	T4839	T4833	punct	),to
R2918	T4840	T4833	prep	from,to
R2919	T4841	T4842	nmod	Acdp1,terminals
R292	T598	T594	conj	characterized,cloned
R2920	T4842	T4840	pobj	terminals,from
R2921	T4843	T4842	nmod	N,terminals
R2922	T4844	T4843	punct	-,N
R2923	T4845	T4843	cc	and,N
R2924	T4846	T4843	conj	C,N
R2925	T4847	T4842	punct	-,terminals
R2926	T4848	T4842	cc	and,terminals
R2927	T4849	T4850	det	the,domain
R2928	T4850	T4842	conj	domain,terminals
R2929	T4851	T4850	amod	conserved,domain
R293	T599	T600	det	a,family
R2930	T4852	T4850	punct	(,domain
R2931	T4853	T4850	appos	ACD,domain
R2932	T4854	T4831	punct	),used
R2933	T4855	T4831	auxpass	were,used
R2934	T4856	T4831	prep	for,used
R2935	T4857	T4856	pobj	generation,for
R2936	T4858	T4857	prep	of,generation
R2937	T4859	T4858	pobj	antibodies,of
R2938	T4860	T4861	advmod	specifically,for
R2939	T4861	T4856	prep	for,for
R294	T600	T598	dobj	family,characterized
R2940	T4862	T4861	pobj	Acdp1,for
R2941	T4863	T4862	cc	and,Acdp1
R2942	T4864	T4865	det	all,members
R2943	T4865	T4862	conj	members,Acdp1
R2944	T4866	T4865	compound	Acdp,members
R2945	T4867	T4868	mark	as,reported
R2946	T4868	T4831	advcl	reported,used
R2947	T4869	T4831	punct	", ",used
R2948	T4870	T4831	advmod	respectively,used
R2949	T4871	T4872	punct	[,5
R295	T601	T600	amod	novel,family
R2950	T4872	T4831	parataxis	5,used
R2951	T4873	T4872	punct	],5
R2952	T4874	T4831	punct	.,used
R2953	T4876	T4877	compound	Western,blots
R2954	T4877	T4878	nsubjpass	blots,carried
R2955	T4879	T4878	auxpass	were,carried
R2956	T4880	T4878	prt	out,carried
R2957	T4881	T4878	advcl	Using,carried
R2958	T4882	T4881	dobj	ECL,Using
R2959	T4883	T4884	punct	(,PIERCE
R296	T602	T600	compound	gene,family
R2960	T4884	T4882	parataxis	PIERCE,ECL
R2961	T4885	T4884	punct	),PIERCE
R2962	T4886	T4887	mark	as,described
R2963	T4887	T4878	advcl	described,carried
R2964	T4888	T4887	advmod	previously,described
R2965	T4889	T4890	punct	[,1
R2966	T4890	T4878	parataxis	1,carried
R2967	T4891	T4890	punct	],1
R2968	T4892	T4878	punct	.,carried
R2969	T4894	T4895	det	The,membranes
R297	T603	T600	acl	named,family
R2970	T4895	T4896	nsubjpass	membranes,washed
R2971	T4897	T4896	auxpass	were,washed
R2972	T4898	T4896	advmod	extensively,washed
R2973	T4899	T4896	prep	after,washed
R2974	T4900	T4899	pobj	incubation,after
R2975	T4901	T4900	prep	with,incubation
R2976	T4902	T4903	amod	primary,antibodies
R2977	T4903	T4901	pobj	antibodies,with
R2978	T4904	T4902	cc	and,primary
R2979	T4905	T4902	conj	secondary,primary
R298	T604	T605	amod	ancient,protein
R2980	T4906	T4896	cc	and,washed
R2981	T4907	T4908	auxpass	were,developed
R2982	T4908	T4896	conj	developed,washed
R2983	T4909	T4908	advmod	then,developed
R2984	T4910	T4908	prep	with,developed
R2985	T4911	T4912	compound	X-ray,films
R2986	T4912	T4910	pobj	films,with
R2987	T4913	T4912	prep	with,films
R2988	T4914	T4915	amod	optimal,time
R2989	T4915	T4913	pobj	time,with
R299	T605	T603	oprd	protein,named
R2990	T4916	T4915	compound	exposure,time
R2991	T4917	T4896	punct	.,washed
R2992	T4941	T4942	compound	Chromosome,localization
R2993	T4944	T4945	det	The,panel
R2994	T4945	T4950	nsubjpass	panel,used
R2995	T4946	T4945	compound	T31,panel
R2996	T4947	T4945	compound	mouse,panel
R2997	T4948	T4945	compound	radiation,panel
R2998	T4949	T4945	compound	hybrid,panel
R2999	T4951	T4945	prep	from,panel
R3	T219	T217	cc	and,cloning
R30	T251	T232	punct	.,cloned
R300	T606	T605	amod	conserved,protein
R3000	T4952	T4953	compound	Research,Genetics
R3001	T4953	T4951	pobj	Genetics,from
R3002	T4954	T4950	auxpass	was,used
R3003	T4955	T4956	aux	to,map
R3004	T4956	T4950	advcl	map,used
R3005	T4957	T4958	det	the,location
R3006	T4958	T4956	dobj	location,map
R3007	T4959	T4958	compound	chromosome,location
R3008	T4960	T4958	prep	of,location
R3009	T4961	T4962	det	each,member
R301	T607	T605	compound	domain,protein
R3010	T4962	T4960	pobj	member,of
R3011	T4963	T4962	compound	Acdp,member
R3012	T4964	T4950	punct	.,used
R3013	T4966	T4967	nsubjpass	Primers,used
R3014	T4968	T4966	prep	from,Primers
R3015	T4969	T4970	nummod	3,UTR
R3016	T4970	T4968	pobj	UTR,from
R3017	T4971	T4969	punct	',3
R3018	T4972	T4970	prep	of,UTR
R3019	T4973	T4974	det	each,member
R302	T608	T605	punct	(,protein
R3020	T4974	T4972	pobj	member,of
R3021	T4975	T4974	compound	Acdp,member
R3022	T4976	T4967	auxpass	were,used
R3023	T4977	T4978	aux	to,amplify
R3024	T4978	T4967	advcl	amplify,used
R3025	T4979	T4980	det	the,clones
R3026	T4980	T4978	dobj	clones,amplify
R3027	T4981	T4980	nummod	100,clones
R3028	T4982	T4980	nmod	radiation,clones
R3029	T4983	T4980	amod	hybrid,clones
R303	T609	T605	appos	ACDP,protein
R3030	T4984	T4980	acl	representing,clones
R3031	T4985	T4986	det	the,genome
R3032	T4986	T4984	dobj	genome,representing
R3033	T4987	T4986	compound	mouse,genome
R3034	T4988	T4967	punct	.,used
R3035	T4990	T4991	det	The,data
R3036	T4991	T4992	nsubjpass	data,submitted
R3037	T4993	T4992	auxpass	were,submitted
R3038	T4994	T4992	prep	to,submitted
R3039	T4995	T4996	det	the,Database
R304	T610	T605	punct	),protein
R3040	T4996	T4994	pobj	Database,to
R3041	T4997	T4998	compound	Jackson,Laboratory
R3042	T4998	T4996	compound	Laboratory,Database
R3043	T4999	T5000	compound	Mouse,Hybrid
R3044	T5000	T4996	compound	Hybrid,Database
R3045	T5001	T5000	compound	Radiation,Hybrid
R3046	T5002	T4992	prep	for,submitted
R3047	T5003	T5002	pobj	analysis,for
R3048	T5004	T4992	punct	.,submitted
R3049	T5023	T5024	compound	Sequence,analyses
R305	T611	T612	dep	which,encodes
R3050	T5026	T5027	compound	Sequence,assembly
R3051	T5027	T5028	nsubjpass	assembly,performed
R3052	T5029	T5028	auxpass	was,performed
R3053	T5030	T5028	prep	with,performed
R3054	T5031	T5032	compound	program,Sequencher
R3055	T5032	T5030	pobj	Sequencher,with
R3056	T5033	T5034	punct	(,Corp
R3057	T5034	T5032	parataxis	Corp,Sequencher
R3058	T5035	T5034	compound	Gene,Corp
R3059	T5036	T5034	compound	Codes,Corp
R306	T612	T600	relcl	encodes,family
R3060	T5037	T5034	punct	),Corp
R3061	T5038	T5028	punct	.,performed
R3062	T5040	T5041	nmod	Protein,homology
R3063	T5041	T5044	compound	homology,searches
R3064	T5042	T5040	cc	and,Protein
R3065	T5043	T5040	conj	DNA,Protein
R3066	T5044	T5045	nsubjpass	searches,carried
R3067	T5046	T5045	auxpass	were,carried
R3068	T5047	T5045	prt	out,carried
R3069	T5048	T5045	prep	with,carried
R307	T613	T614	nummod	four,members
R3070	T5049	T5050	nmod	tblastn,programs
R3071	T5050	T5048	pobj	programs,with
R3072	T5051	T5049	punct	", ",tblastn
R3073	T5052	T5049	conj	tblastx,tblastn
R3074	T5053	T5052	punct	", ",tblastx
R3075	T5054	T5052	conj	blastp,tblastx
R3076	T5055	T5054	cc	and,blastp
R3077	T5056	T5054	conj	blastn,blastp
R3078	T5057	T5045	punct	.,carried
R3079	T5059	T5060	amod	Multiple,alignments
R308	T614	T612	dobj	members,encodes
R3080	T5060	T5062	nsubjpass	alignments,performed
R3081	T5061	T5060	compound	sequence,alignments
R3082	T5063	T5062	auxpass	were,performed
R3083	T5064	T5062	prep	with,performed
R3084	T5065	T5066	nmod	GeneDoc,alignment
R3085	T5066	T5064	pobj	alignment,with
R3086	T5067	T5065	cc	and,GeneDoc
R3087	T5068	T5065	conj	pairwise,GeneDoc
R3088	T5069	T5066	compound	sequence,alignment
R3089	T5070	T5062	punct	.,performed
R309	T615	T614	compound	protein,members
R3090	T5072	T5073	amod	Multiple,programs
R3091	T5073	T5074	nsubjpass	programs,used
R3092	T5075	T5073	prep	including,programs
R3093	T5076	T5077	compound	BCM,Launcher
R3094	T5077	T5075	pobj	Launcher,including
R3095	T5078	T5077	compound	Search,Launcher
R3096	T5079	T5077	punct	", ",Launcher
R3097	T5080	T5077	conj	ProfileScan,Launcher
R3098	T5081	T5080	punct	", ",ProfileScan
R3099	T5082	T5083	compound	sequence,search
R31	T253	T254	aux	To,facilitate
R310	T616	T612	prep	in,encodes
R3100	T5083	T5080	conj	search,ProfileScan
R3101	T5084	T5083	compound	motif,search
R3102	T5085	T5083	punct	", ",search
R3103	T5086	T5083	conj	ExPASy,search
R3104	T5087	T5086	cc	and,ExPASy
R3105	T5088	T5086	conj	3Dpssm,ExPASy
R3106	T5089	T5074	auxpass	were,used
R3107	T5090	T5074	prep	for,used
R3108	T5091	T5090	pcomp	searching,for
R3109	T5092	T5093	compound	sequence,features
R311	T617	T616	pobj	humans,in
R3110	T5093	T5091	dobj	features,searching
R3111	T5094	T5093	prep	of,features
R3112	T5095	T5096	amod	known,protein
R3113	T5096	T5094	pobj	protein,of
R3114	T5097	T5074	punct	.,used
R3115	T5099	T5100	amod	Phylogenetic,tree
R3116	T5100	T5101	nsubjpass	tree,constructed
R3117	T5102	T5101	auxpass	was,constructed
R3118	T5103	T5101	agent	by,constructed
R3119	T5104	T5105	compound	Clustalw,program
R312	T618	T619	punct	[,1
R3120	T5105	T5103	pobj	program,by
R3121	T5106	T5107	punct	(,version
R3122	T5107	T5105	parataxis	version,program
R3123	T5108	T5107	nummod	1.81,version
R3124	T5109	T5107	punct	),version
R3125	T5110	T5101	advcl	using,constructed
R3126	T5111	T5112	nmod	UPGMA,algorithm
R3127	T5112	T5110	dobj	algorithm,using
R3128	T5113	T5111	punct	(,UPGMA
R3129	T5114	T5115	amod	Unweighted,Method
R313	T619	T598	parataxis	1,characterized
R3130	T5115	T5111	appos	Method,UPGMA
R3131	T5116	T5115	compound	Pair,Method
R3132	T5117	T5115	compound	Group,Method
R3133	T5118	T5115	acl	using,Method
R3134	T5119	T5120	compound	Arithmetic,averages
R3135	T5120	T5118	dobj	averages,using
R3136	T5121	T5112	punct	),algorithm
R3137	T5122	T5123	punct	[,14
R3138	T5123	T5101	parataxis	14,constructed
R3139	T5124	T5123	punct	],14
R314	T620	T619	punct	],1
R3140	T5125	T5101	punct	.,constructed
R3141	T5264	T5265	amod	Neuronal,preparation
R3142	T5266	T5265	compound	cell,preparation
R3143	T5267	T5265	cc	and,preparation
R3144	T5268	T5265	conj	immunostanining,preparation
R3145	T5270	T5271	amod	Hippocampal,cultures
R3146	T5271	T5273	nsubjpass	cultures,prepared
R3147	T5272	T5271	compound	neuron,cultures
R3148	T5274	T5273	auxpass	were,prepared
R3149	T5275	T5276	mark	as,reported
R315	T621	T594	punct	.,cloned
R3150	T5276	T5273	advcl	reported,prepared
R3151	T5277	T5276	advmod	previously,reported
R3152	T5278	T5279	punct	[,6
R3153	T5279	T5273	parataxis	6,prepared
R3154	T5280	T5279	punct	],6
R3155	T5281	T5273	punct	.,prepared
R3156	T5283	T5284	prep	In,dissected
R3157	T5285	T5283	pobj	brief,In
R3158	T5286	T5284	punct	", ",dissected
R3159	T5287	T5288	det	the,hippocampuses
R316	T623	T624	nsubj	We,found
R3160	T5288	T5284	nsubjpass	hippocampuses,dissected
R3161	T5289	T5284	auxpass	were,dissected
R3162	T5290	T5284	prt	out,dissected
R3163	T5291	T5284	prep	from,dissected
R3164	T5292	T5293	compound	mouse,embryos
R3165	T5293	T5291	pobj	embryos,from
R3166	T5294	T5284	prep	at,dissected
R3167	T5295	T5296	nummod	16,days
R3168	T5296	T5294	pobj	days,at
R3169	T5297	T5298	advmod	in,utero
R317	T625	T626	mark	that,is
R3170	T5298	T5296	advmod	utero,days
R3171	T5299	T5284	punct	.,dissected
R3172	T5301	T5302	det	The,tissues
R3173	T5302	T5303	nsubjpass	tissues,incubated
R3174	T5304	T5303	auxpass	were,incubated
R3175	T5305	T5303	advmod	then,incubated
R3176	T5306	T5303	prep	for,incubated
R3177	T5307	T5308	nummod	20,min
R3178	T5308	T5306	pobj	min,for
R3179	T5309	T5303	prep	at,incubated
R318	T626	T624	ccomp	is,found
R3180	T5310	T5311	nummod	37,°C
R3181	T5311	T5309	pobj	°C,at
R3182	T5312	T5303	prep	in,incubated
R3183	T5313	T5312	pobj	MEM,in
R3184	T5314	T5313	punct	(,MEM
R3185	T5315	T5316	amod	minimum,medium
R3186	T5316	T5313	appos	medium,MEM
R3187	T5317	T5316	amod	essential,medium
R3188	T5318	T5313	punct	),MEM
R3189	T5319	T5313	acl	modified,MEM
R319	T627	T628	det	this,family
R3190	T5320	T5319	prep	for,modified
R3191	T5321	T5322	compound	suspension,culture
R3192	T5322	T5320	pobj	culture,for
R3193	T5323	T5324	punct	(,Technologies
R3194	T5324	T5313	parataxis	Technologies,MEM
R3195	T5325	T5324	compound	Life,Technologies
R3196	T5326	T5324	punct	),Technologies
R3197	T5327	T5313	cc	plus,MEM
R3198	T5328	T5329	nummod	0.25,%
R3199	T5329	T5330	compound	%,trypsin
R32	T254	T255	advcl	facilitate,cloned
R320	T628	T626	nsubj	family,is
R3200	T5330	T5313	conj	trypsin,MEM
R3201	T5331	T5332	punct	(,Technologies
R3202	T5332	T5330	parataxis	Technologies,trypsin
R3203	T5333	T5332	compound	Life,Technologies
R3204	T5334	T5332	punct	),Technologies
R3205	T5335	T5303	punct	.,incubated
R3206	T5337	T5338	det	The,neurons
R3207	T5338	T5341	nsubjpass	neurons,plated
R3208	T5339	T5338	amod	dissociated,neurons
R3209	T5340	T5338	amod	hippocampal,neurons
R321	T629	T628	compound	gene,family
R3210	T5342	T5341	auxpass	were,plated
R3211	T5343	T5341	prep	on,plated
R3212	T5344	T5345	compound	glass,coverslips
R3213	T5345	T5343	pobj	coverslips,on
R3214	T5346	T5345	acl	coated,coverslips
R3215	T5347	T5346	prep	with,coated
R3216	T5348	T5349	det	a,monolayer
R3217	T5349	T5347	pobj	monolayer,with
R3218	T5350	T5349	amod	confluent,monolayer
R3219	T5351	T5349	prep	of,monolayer
R322	T630	T631	advmod	evolutionarily,conserved
R3220	T5352	T5353	nmod	mouse,astrocytes
R3221	T5353	T5351	pobj	astrocytes,of
R3222	T5354	T5353	amod	cortical,astrocytes
R3223	T5355	T5353	acl	obtained,astrocytes
R3224	T5356	T5357	mark	as,described
R3225	T5357	T5355	advcl	described,obtained
R3226	T5358	T5357	advmod	below,described
R3227	T5359	T5341	punct	.,plated
R3228	T5361	T5362	det	The,neurons
R3229	T5362	T5363	nsubjpass	neurons,maintained
R323	T631	T626	acomp	conserved,is
R3230	T5364	T5363	auxpass	were,maintained
R3231	T5365	T5363	prep	at,maintained
R3232	T5366	T5367	nummod	37,°C
R3233	T5367	T5365	pobj	°C,at
R3234	T5368	T5363	prep	in,maintained
R3235	T5369	T5370	det	a,atmosphere
R3236	T5370	T5368	pobj	atmosphere,in
R3237	T5371	T5370	amod	humidified,atmosphere
R3238	T5372	T5370	prep	with,atmosphere
R3239	T5373	T5374	nummod	5,%
R324	T632	T626	prep	in,is
R3240	T5374	T5375	compound	%,CO2
R3241	T5375	T5372	pobj	CO2,with
R3242	T5376	T5363	punct	.,maintained
R3243	T5378	T5379	amod	Cortical,astrocytes
R3244	T5379	T5380	nsubjpass	astrocytes,grown
R3245	T5381	T5379	acl	dissociated,astrocytes
R3246	T5382	T5381	prep	from,dissociated
R3247	T5383	T5384	amod	newborn,mouse
R3248	T5384	T5385	compound	mouse,cortices
R3249	T5385	T5382	pobj	cortices,from
R325	T633	T634	amod	diverse,species
R3250	T5386	T5380	auxpass	were,grown
R3251	T5387	T5380	prep	in,grown
R3252	T5388	T5389	compound	culture,flasks
R3253	T5389	T5387	pobj	flasks,in
R3254	T5390	T5380	prep	at,grown
R3255	T5391	T5392	nummod	37,°C
R3256	T5392	T5390	pobj	°C,at
R3257	T5393	T5380	prep	in,grown
R3258	T5394	T5395	det	a,atmosphere
R3259	T5395	T5393	pobj	atmosphere,in
R326	T634	T632	pobj	species,in
R3260	T5396	T5395	amod	humidified,atmosphere
R3261	T5397	T5395	prep	with,atmosphere
R3262	T5398	T5399	nummod	5,%
R3263	T5399	T5400	compound	%,CO2
R3264	T5400	T5397	pobj	CO2,with
R3265	T5401	T5380	prep	until,grown
R3266	T5402	T5401	pcomp	confluent,until
R3267	T5403	T5380	punct	.,grown
R3268	T5405	T5406	det	The,cells
R3269	T5406	T5407	nsubjpass	cells,exposed
R327	T635	T634	acl	ranging,species
R3270	T5408	T5407	auxpass	were,exposed
R3271	T5409	T5407	prep	to,exposed
R3272	T5410	T5411	quantmod	10,5
R3273	T5411	T5413	nummod	5,M
R3274	T5412	T5411	punct	-,5
R3275	T5413	T5414	compound	M,arabinoside
R3276	T5414	T5409	pobj	arabinoside,to
R3277	T5415	T5414	compound	cytosine,arabinoside
R3278	T5416	T5417	punct	(,Sigma
R3279	T5417	T5414	parataxis	Sigma,arabinoside
R328	T636	T635	prep	from,ranging
R3280	T5418	T5417	punct	),Sigma
R3281	T5419	T5407	cc	and,exposed
R3282	T5420	T5407	conj	cultured,exposed
R3283	T5421	T5420	prep	for,cultured
R3284	T5422	T5423	amod	additional,hrs
R3285	T5423	T5421	pobj	hrs,for
R3286	T5424	T5425	quantmod	12,24
R3287	T5425	T5423	nummod	24,hrs
R3288	T5426	T5425	punct	–,24
R3289	T5427	T5420	prep	at,cultured
R329	T637	T636	pobj	bacteria,from
R3290	T5428	T5429	nummod	37,°C
R3291	T5429	T5427	pobj	°C,at
R3292	T5430	T5407	punct	.,exposed
R3293	T5432	T5433	prep	After,used
R3294	T5434	T5432	pobj	remove,After
R3295	T5435	T5434	prep	of,remove
R3296	T5436	T5437	det	the,media
R3297	T5437	T5435	pobj	media,of
R3298	T5438	T5434	prep	with,remove
R3299	T5439	T5440	amod	cellular,debris
R33	T256	T257	det	the,study
R330	T638	T637	punct	", ",bacteria
R3300	T5440	T5438	pobj	debris,with
R3301	T5441	T5433	punct	", ",used
R3302	T5442	T5443	det	the,cells
R3303	T5443	T5433	nsubjpass	cells,used
R3304	T5444	T5433	auxpass	were,used
R3305	T5445	T5433	prep	for,used
R3306	T5446	T5445	pcomp	coating,for
R3307	T5447	T5446	dobj	coverslips,coating
R3308	T5448	T5433	punct	.,used
R3309	T5450	T5451	prep	For,fixed
R331	T639	T637	conj	yeast,bacteria
R3310	T5452	T5450	pobj	immunostaining,For
R3311	T5453	T5451	punct	", ",fixed
R3312	T5454	T5455	amod	neuronal,cells
R3313	T5455	T5451	nsubjpass	cells,fixed
R3314	T5456	T5455	prep	on,cells
R3315	T5457	T5458	det	the,coverslips
R3316	T5458	T5456	pobj	coverslips,on
R3317	T5459	T5451	auxpass	were,fixed
R3318	T5460	T5451	advmod	first,fixed
R3319	T5461	T5451	prep	in,fixed
R332	T640	T639	punct	", ",yeast
R3320	T5462	T5461	pobj	PBS,in
R3321	T5463	T5462	acl	containing,PBS
R3322	T5464	T5465	nummod	4,%
R3323	T5465	T5466	compound	%,paraformaldehyde
R3324	T5466	T5463	dobj	paraformaldehyde,containing
R3325	T5467	T5466	punct	(,paraformaldehyde
R3326	T5468	T5466	appos	PFA,paraformaldehyde
R3327	T5469	T5463	punct	),containing
R3328	T5470	T5463	prep	for,containing
R3329	T5471	T5472	nummod	12,hrs
R333	T641	T642	compound	C.,elegans
R3330	T5472	T5470	pobj	hrs,for
R3331	T5473	T5463	prep	at,containing
R3332	T5474	T5475	nummod	4,°C
R3333	T5475	T5473	pobj	°C,at
R3334	T5476	T5451	cc	and,fixed
R3335	T5477	T5478	advmod	then,incubated
R3336	T5478	T5451	conj	incubated,fixed
R3337	T5479	T5478	prep	in,incubated
R3338	T5480	T5481	det	a,solution
R3339	T5481	T5479	pobj	solution,in
R334	T642	T639	conj	elegans,yeast
R3340	T5482	T5481	acl	containing,solution
R3341	T5483	T5484	nummod	4,%
R3342	T5484	T5485	compound	%,PFA
R3343	T5485	T5482	dobj	PFA,containing
R3344	T5486	T5485	cc	and,PFA
R3345	T5487	T5488	nummod	0.4,%
R3346	T5488	T5489	compound	%,100
R3347	T5489	T5485	conj	100,PFA
R3348	T5490	T5489	compound	Triton,100
R3349	T5491	T5489	compound	X,100
R335	T643	T642	punct	", ",elegans
R3350	T5492	T5489	punct	-,100
R3351	T5493	T5478	prep	at,incubated
R3352	T5494	T5495	nummod	4,°C
R3353	T5495	T5493	pobj	°C,at
R3354	T5496	T5478	prep	for,incubated
R3355	T5497	T5498	nummod	1,hr
R3356	T5498	T5496	pobj	hr,for
R3357	T5499	T5451	punct	.,fixed
R3358	T5501	T5502	prep	After,incubated
R3359	T5503	T5501	pcomp	washing,After
R336	T644	T642	cc	and,elegans
R3360	T5504	T5503	prep	with,washing
R3361	T5505	T5504	pobj	PBS,with
R3362	T5506	T5507	nummod	three,times
R3363	T5507	T5503	npadvmod	times,washing
R3364	T5508	T5502	punct	", ",incubated
R3365	T5509	T5510	det	the,cells
R3366	T5510	T5502	nsubjpass	cells,incubated
R3367	T5511	T5502	auxpass	were,incubated
R3368	T5512	T5502	prep	with,incubated
R3369	T5513	T5514	det	a,solution
R337	T645	T646	compound	D.,melanogaster
R3370	T5514	T5512	pobj	solution,with
R3371	T5515	T5514	amod	blocking,solution
R3372	T5516	T5514	acl	containing,solution
R3373	T5517	T5518	nummod	1,serum
R3374	T5518	T5516	dobj	serum,containing
R3375	T5519	T5520	punct	:,30
R3376	T5520	T5517	prep	30,1
R3377	T5521	T5518	amod	normal,serum
R3378	T5522	T5518	compound	goat,serum
R3379	T5523	T5502	punct	", ",incubated
R338	T646	T642	conj	melanogaster,elegans
R3380	T5524	T5502	cc	and,incubated
R3381	T5525	T5526	advmod	subsequently,incubated
R3382	T5526	T5502	conj	incubated,incubated
R3383	T5527	T5526	prep	with,incubated
R3384	T5528	T5529	det	a,antibody
R3385	T5529	T5527	pobj	antibody,with
R3386	T5530	T5529	nmod	rabbit,antibody
R3387	T5531	T5529	amod	polyclonal,antibody
R3388	T5532	T5529	amod	anti-ACDP,antibody
R3389	T5533	T5534	punct	(,1
R339	T647	T636	prep	to,from
R3390	T5534	T5529	parataxis	1,antibody
R3391	T5535	T5536	punct	:,3000
R3392	T5536	T5534	prep	3000,1
R3393	T5537	T5534	punct	),1
R3394	T5538	T5526	advmod	overnight,incubated
R3395	T5539	T5526	prep	at,incubated
R3396	T5540	T5541	nummod	4,°C
R3397	T5541	T5539	pobj	°C,at
R3398	T5542	T5502	punct	.,incubated
R3399	T5544	T5545	prep	After,incubated
R34	T257	T254	dobj	study,facilitate
R340	T648	T647	pobj	mammals,to
R3400	T5546	T5547	amod	extensive,washing
R3401	T5547	T5544	pobj	washing,After
R3402	T5548	T5547	prep	with,washing
R3403	T5549	T5550	nummod	1,%
R3404	T5550	T5551	compound	%,serum
R3405	T5551	T5553	compound	serum,solution
R3406	T5552	T5551	compound	goat,serum
R3407	T5553	T5548	pobj	solution,with
R3408	T5554	T5553	compound	PBS,solution
R3409	T5555	T5545	punct	", ",incubated
R341	T649	T624	punct	.,found
R3410	T5556	T5557	det	the,cells
R3411	T5557	T5545	nsubjpass	cells,incubated
R3412	T5558	T5545	auxpass	were,incubated
R3413	T5559	T5545	prep	with,incubated
R3414	T5560	T5561	det	an,antibody
R3415	T5561	T5559	pobj	antibody,with
R3416	T5562	T5561	nmod	Alex,antibody
R3417	T5563	T5562	nummod	488,Alex
R3418	T5564	T5561	amod	conjugated,antibody
R3419	T5565	T5561	amod	secondary,antibody
R342	T651	T652	det	The,conservation
R3420	T5566	T5567	punct	(,Probes
R3421	T5567	T5561	parataxis	Probes,antibody
R3422	T5568	T5567	dep	1,Probes
R3423	T5569	T5570	punct	:,100
R3424	T5570	T5568	prep	100,1
R3425	T5571	T5568	prep	in,1
R3426	T5572	T5573	nummod	1,%
R3427	T5573	T5574	compound	%,serum
R3428	T5574	T5576	compound	serum,solution
R3429	T5575	T5574	compound	goat,serum
R343	T652	T654	nsubj	conservation,imply
R3430	T5576	T5571	pobj	solution,in
R3431	T5577	T5576	compound	PBS,solution
R3432	T5578	T5567	punct	", ",Probes
R3433	T5579	T5567	compound	Molecular,Probes
R3434	T5580	T5567	punct	),Probes
R3435	T5581	T5545	prep	for,incubated
R3436	T5582	T5583	nummod	3,hrs
R3437	T5583	T5581	pobj	hrs,for
R3438	T5584	T5545	prep	at,incubated
R3439	T5585	T5586	compound	room,temperature
R344	T653	T652	compound	sequence,conservation
R3440	T5586	T5584	pobj	temperature,at
R3441	T5587	T5545	punct	.,incubated
R3442	T5589	T5590	prep	Following,slipped
R3443	T5591	T5592	amod	final,washes
R3444	T5592	T5589	pobj	washes,Following
R3445	T5593	T5592	prep	with,washes
R3446	T5594	T5595	nummod	1,%
R3447	T5595	T5596	compound	%,serum
R3448	T5596	T5598	compound	serum,solution
R3449	T5597	T5596	compound	goat,serum
R345	T655	T652	cc	and,conservation
R3450	T5598	T5593	pobj	solution,with
R3451	T5599	T5598	compound	PBS,solution
R3452	T5600	T5590	punct	", ",slipped
R3453	T5601	T5602	det	the,cells
R3454	T5602	T5590	nsubjpass	cells,slipped
R3455	T5603	T5602	amod	neuronal,cells
R3456	T5604	T5602	prep	on,cells
R3457	T5605	T5606	det	the,coverslips
R3458	T5606	T5604	pobj	coverslips,on
R3459	T5607	T5590	auxpass	were,slipped
R346	T656	T657	det	the,presence
R3460	T5608	T5590	dep	cover,slipped
R3461	T5609	T5590	punct	-,slipped
R3462	T5610	T5590	prep	with,slipped
R3463	T5611	T5612	det	a,medium
R3464	T5612	T5610	pobj	medium,with
R3465	T5613	T5614	npadvmod	glycerol,based
R3466	T5614	T5612	amod	based,medium
R3467	T5615	T5614	punct	-,based
R3468	T5616	T5612	amod	anti-photobleach,medium
R3469	T5617	T5590	punct	.,slipped
R347	T657	T652	conj	presence,conservation
R3470	T5619	T5620	det	The,cells
R3471	T5620	T5621	nsubjpass	cells,viewed
R3472	T5622	T5621	auxpass	were,viewed
R3473	T5623	T5621	prep	under,viewed
R3474	T5624	T5625	det	a,microscope
R3475	T5625	T5623	pobj	microscope,under
R3476	T5626	T5625	amod	confocal,microscope
R3477	T5627	T5628	punct	(,Zeiss
R3478	T5628	T5625	parataxis	Zeiss,microscope
R3479	T5629	T5628	compound	Carl,Zeiss
R348	T658	T657	prep	of,presence
R3480	T5630	T5628	punct	),Zeiss
R3481	T5631	T5621	punct	.,viewed
R3482	T5633	T5634	nsubjpass	Images,captured
R3483	T5635	T5634	auxpass	were,captured
R3484	T5636	T5634	prep	with,captured
R3485	T5637	T5638	det	a,camera
R3486	T5638	T5636	pobj	camera,with
R3487	T5639	T5638	compound	CCD,camera
R3488	T5640	T5634	cc	and,captured
R3489	T5641	T5634	conj	acquired,captured
R349	T659	T660	amod	multiple,members
R3490	T5642	T5641	prep	by,acquired
R3491	T5643	T5644	det	the,software
R3492	T5644	T5642	pobj	software,by
R3493	T5645	T5644	compound	Scion,software
R3494	T5646	T5644	compound	Image,software
R3495	T5647	T5648	punct	(,Corporation
R3496	T5648	T5644	parataxis	Corporation,software
R3497	T5649	T5648	compound	Scion,Corporation
R3498	T5650	T5648	punct	", ",Corporation
R3499	T5651	T5648	npadvmod	Frederick,Corporation
R35	T258	T257	amod	functional,study
R350	T660	T658	pobj	members,of
R3500	T5652	T5648	punct	", ",Corporation
R3501	T5653	T5648	npadvmod	MD,Corporation
R3502	T5654	T5648	punct	),Corporation
R3503	T5655	T5634	punct	.,captured
R3504	T5677	T5678	compound	Gene,bank
R3505	T5678	T5679	compound	bank,number
R3506	T5680	T5679	compound	accession,number
R3507	T5682	T5683	det	The,sequences
R3508	T5683	T5685	nsubjpass	sequences,deposited
R3509	T5684	T5683	compound	cDNA,sequences
R351	T661	T657	prep	within,presence
R3510	T5686	T5683	prep	for,sequences
R3511	T5687	T5688	det	the,family
R3512	T5688	T5686	pobj	family,for
R3513	T5689	T5688	compound	Acdp,family
R3514	T5690	T5688	compound	gene,family
R3515	T5691	T5685	aux	have,deposited
R3516	T5692	T5685	advmod	already,deposited
R3517	T5693	T5685	auxpass	been,deposited
R3518	T5694	T5685	prep	in,deposited
R3519	T5695	T5696	compound	gene,bank
R352	T662	T663	det	a,species
R3520	T5696	T5694	pobj	bank,in
R3521	T5697	T5685	prep	with,deposited
R3522	T5698	T5699	compound	accession,AF202994
R3523	T5699	T5697	pobj	AF202994,with
R3524	T5700	T5699	compound	numbers,AF202994
R3525	T5701	T5699	punct	(,AF202994
R3526	T5702	T5699	appos	Acdp1,AF202994
R3527	T5703	T5699	punct	),AF202994
R3528	T5704	T5699	punct	", ",AF202994
R3529	T5705	T5699	conj	AF216961,AF202994
R353	T663	T661	pobj	species,within
R3530	T5706	T5705	punct	(,AF216961
R3531	T5707	T5705	appos	Acdp2,AF216961
R3532	T5708	T5705	punct	),AF216961
R3533	T5709	T5705	punct	", ",AF216961
R3534	T5710	T5705	conj	AF216964,AF216961
R3535	T5711	T5710	punct	(,AF216964
R3536	T5712	T5710	appos	Acdp3,AF216964
R3537	T5713	T5710	punct	),AF216964
R3538	T5714	T5710	cc	and,AF216964
R3539	T5715	T5710	conj	AF216963,AF216964
R354	T664	T654	aux	may,imply
R3540	T5716	T5715	punct	(,AF216963
R3541	T5717	T5715	appos	Acdp4,AF216963
R3542	T5718	T5699	punct	),AF202994
R3543	T5719	T5685	punct	.,deposited
R3544	T5731	T5732	compound	Northern,blot
R3545	T5732	T5733	compound	blot,analyses
R3546	T5734	T5733	prep	of,analyses
R3547	T5735	T5736	det	the,family
R3548	T5736	T5734	pobj	family,of
R3549	T5737	T5736	compound	Acdp,family
R355	T665	T666	amod	functional,importance
R3550	T5738	T5736	compound	gene,family
R3551	T5739	T5733	punct	.,analyses
R3552	T5741	T5742	compound	S.,muscle
R3553	T5742	T5743	nsubj	muscle,represents
R3554	T5744	T5745	amod	skeletal,muscle
R3555	T5745	T5743	dobj	muscle,represents
R3556	T5746	T5745	punct	", ",muscle
R3557	T5747	T5745	appos	Sm,muscle
R3558	T5748	T5743	punct	.,represents
R3559	T5750	T5751	nsubj	Int.,represents
R356	T666	T654	dobj	importance,imply
R3560	T5752	T5753	amod	small,intestine
R3561	T5753	T5751	dobj	intestine,represents
R3562	T5754	T5751	punct	.,represents
R3563	T5756	T5757	amod	Multiple,Choice
R3564	T5757	T5758	compound	Choice,Blot
R3565	T5758	T5760	compound	Blot,filters
R3566	T5759	T5758	compound	Northern,Blot
R3567	T5760	T5761	nsubjpass	filters,purchased
R3568	T5762	T5761	auxpass	were,purchased
R3569	T5763	T5761	prep	from,purchased
R357	T667	T666	acl	associated,importance
R3570	T5764	T5763	pobj	Origene,from
R3571	T5765	T5761	punct	.,purchased
R3572	T5826	T5827	compound	Amino,acid
R3573	T5827	T5828	compound	acid,sequence
R3574	T5828	T5829	compound	sequence,alignment
R3575	T5830	T5829	compound	homology,alignment
R3576	T5831	T5829	prep	for,alignment
R3577	T5832	T5831	pobj	all,for
R3578	T5833	T5832	prep	of,all
R3579	T5834	T5835	det	the,genes
R358	T668	T667	prep	with,associated
R3580	T5835	T5833	pobj	genes,of
R3581	T5836	T5835	nmod	ACDP,genes
R3582	T5837	T5836	cc	and,ACDP
R3583	T5838	T5836	conj	Acdp,ACDP
R3584	T5839	T5835	prep	within,genes
R3585	T5840	T5841	det	the,domain
R3586	T5841	T5839	pobj	domain,within
R3587	T5842	T5841	compound	ACD,domain
R3588	T5843	T5829	punct	.,alignment
R3589	T5845	T5846	det	The,data
R359	T669	T670	det	the,genes
R3590	T5846	T5848	nsubjpass	data,deposited
R3591	T5847	T5846	compound	sequence,data
R3592	T5849	T5846	prep	for,data
R3593	T5850	T5851	det	the,genes
R3594	T5851	T5849	pobj	genes,for
R3595	T5852	T5851	compound	Acdp,genes
R3596	T5853	T5848	aux	have,deposited
R3597	T5854	T5848	auxpass	been,deposited
R3598	T5855	T5848	prep	in,deposited
R3599	T5856	T5855	pobj	GenBank,in
R36	T259	T257	prep	of,study
R360	T670	T668	pobj	genes,with
R3600	T5857	T5848	prep	under,deposited
R3601	T5858	T5859	compound	accession,AF202994
R3602	T5859	T5857	pobj	AF202994,under
R3603	T5860	T5859	compound	number,AF202994
R3604	T5861	T5859	punct	(,AF202994
R3605	T5862	T5859	appos	Acdp1,AF202994
R3606	T5863	T5859	punct	),AF202994
R3607	T5864	T5859	punct	", ",AF202994
R3608	T5865	T5859	conj	AF216961,AF202994
R3609	T5866	T5865	punct	(,AF216961
R361	T671	T654	punct	.,imply
R3610	T5867	T5865	appos	Acdp2,AF216961
R3611	T5868	T5865	punct	),AF216961
R3612	T5869	T5865	punct	", ",AF216961
R3613	T5870	T5865	conj	AF216964,AF216961
R3614	T5871	T5870	punct	(,AF216964
R3615	T5872	T5870	appos	Acdp3,AF216964
R3616	T5873	T5870	punct	),AF216964
R3617	T5874	T5870	cc	and,AF216964
R3618	T5875	T5870	conj	AF216963,AF216964
R3619	T5876	T5875	punct	(,AF216963
R362	T673	T674	aux	To,facilitate
R3620	T5877	T5875	appos	Acdp4,AF216963
R3621	T5878	T5859	punct	),AF202994
R3622	T5879	T5848	punct	.,deposited
R3623	T5881	T5882	amod	Identical,acids
R3624	T5882	T5884	nsubjpass	acids,shaded
R3625	T5883	T5882	compound	amino,acids
R3626	T5885	T5882	cc	or,acids
R3627	T5886	T5887	compound	amino,acids
R3628	T5887	T5882	conj	acids,acids
R3629	T5888	T5887	prep	with,acids
R363	T674	T675	advcl	facilitate,cloned
R3630	T5889	T5890	advmod	very,strong
R3631	T5890	T5891	amod	strong,homologies
R3632	T5891	T5888	pobj	homologies,with
R3633	T5892	T5891	prep	among,homologies
R3634	T5893	T5894	det	all,proteins
R3635	T5894	T5892	pobj	proteins,among
R3636	T5895	T5884	auxpass	were,shaded
R3637	T5896	T5884	oprd	black,shaded
R3638	T5897	T5884	punct	.,shaded
R3639	T5899	T5900	amod	Identical,acids
R364	T676	T677	det	the,analysis
R3640	T5900	T5902	nsubjpass	acids,shaded
R3641	T5901	T5900	compound	amino,acids
R3642	T5903	T5900	cc	or,acids
R3643	T5904	T5905	compound	amino,acids
R3644	T5905	T5900	conj	acids,acids
R3645	T5906	T5905	prep	with,acids
R3646	T5907	T5908	advmod	very,strong
R3647	T5908	T5909	amod	strong,homologies
R3648	T5909	T5906	pobj	homologies,with
R3649	T5910	T5909	prep	in,homologies
R365	T677	T674	dobj	analysis,facilitate
R3650	T5911	T5910	pobj	most,in
R3651	T5912	T5911	prep	of,most
R3652	T5913	T5914	det	the,proteins
R3653	T5914	T5912	pobj	proteins,of
R3654	T5915	T5902	auxpass	were,shaded
R3655	T5916	T5902	oprd	grey,shaded
R3656	T5917	T5902	punct	.,shaded
R3657	T5919	T5920	compound	Dot,lines
R3658	T5920	T5921	nsubj	lines,represent
R3659	T5922	T5921	dobj	gaps,represent
R366	T678	T677	amod	functional,analysis
R3660	T5923	T5922	prep	for,gaps
R3661	T5924	T5925	det	the,alignment
R3662	T5925	T5923	pobj	alignment,for
R3663	T5926	T5921	punct	.,represent
R3664	T6010	T6011	compound	Amino,acid
R3665	T6011	T6012	compound	acid,alignment
R3666	T6013	T6012	compound	sequence,alignment
R3667	T6014	T6012	acl	showing,alignment
R3668	T6015	T6016	det	the,conservation
R3669	T6016	T6014	dobj	conservation,showing
R367	T679	T677	prep	of,analysis
R3670	T6017	T6016	prep	of,conservation
R3671	T6018	T6019	compound	ACD,domain
R3672	T6019	T6017	pobj	domain,of
R3673	T6020	T6014	prep	in,showing
R3674	T6021	T6022	amod	various,species
R3675	T6022	T6020	pobj	species,in
R3676	T6023	T6012	punct	.,alignment
R3677	T6025	T6026	nsubj	Amip3,is
R3678	T6027	T6028	det	a,protein
R3679	T6028	T6026	attr	protein,is
R368	T680	T681	det	the,family
R3680	T6029	T6028	prep	from,protein
R3681	T6030	T6031	compound	Saccharomyces,cerevisiae
R3682	T6031	T6029	pobj	cerevisiae,from
R3683	T6032	T6028	punct	(,protein
R3684	T6033	T6028	appos	NP_014581,protein
R3685	T6034	T6026	punct	),is
R3686	T6035	T6026	punct	.,is
R3687	T6037	T6038	nsubj	CanG,is
R3688	T6039	T6040	det	a,protein
R3689	T6040	T6038	attr	protein,is
R369	T681	T679	pobj	family,of
R3690	T6041	T6040	prep	from,protein
R3691	T6042	T6043	compound	Candida,glabrata
R3692	T6043	T6041	pobj	glabrata,from
R3693	T6044	T6040	punct	(,protein
R3694	T6045	T6040	appos	AAF33142,protein
R3695	T6046	T6038	punct	),is
R3696	T6047	T6038	punct	.,is
R3697	T6049	T6050	nsubj	NeuC,is
R3698	T6051	T6049	punct	(,NeuC
R3699	T6052	T6049	appos	EAA31204,NeuC
R37	T260	T261	det	this,family
R370	T682	T681	compound	ACDP,family
R3700	T6053	T6050	punct	),is
R3701	T6054	T6055	det	a,protein
R3702	T6055	T6050	attr	protein,is
R3703	T6056	T6055	amod	hypothetical,protein
R3704	T6057	T6055	prep	from,protein
R3705	T6058	T6059	compound	Neurospora,crassa
R3706	T6059	T6057	pobj	crassa,from
R3707	T6060	T6050	punct	.,is
R3708	T6062	T6063	nsubj	DroM,is
R3709	T6064	T6065	det	a,product
R371	T683	T681	compound	gene,family
R3710	T6065	T6063	attr	product,is
R3711	T6066	T6065	compound	gene,product
R3712	T6067	T6065	prep	from,product
R3713	T6068	T6069	compound	D.,melanogaster
R3714	T6069	T6067	pobj	melanogaster,from
R3715	T6070	T6063	punct	.,is
R3716	T6072	T6073	det	The,number
R3717	T6073	T6075	nsubj	number,is
R3718	T6074	T6073	compound	accession,number
R3719	T6076	T6073	prep	for,number
R372	T684	T675	punct	", ",cloned
R3720	T6077	T6078	det	this,gene
R3721	T6078	T6076	pobj	gene,for
R3722	T6079	T6075	attr	CG40084,is
R3723	T6080	T6075	prep	in,is
R3724	T6081	T6080	pobj	BDGP,in
R3725	T6082	T6081	punct	(,BDGP
R3726	T6083	T6084	compound	Berkeley,Project
R3727	T6084	T6081	appos	Project,BDGP
R3728	T6085	T6084	compound	Drosophila,Project
R3729	T6086	T6084	compound	Genome,Project
R373	T685	T675	nsubj	we,cloned
R3730	T6087	T6075	punct	),is
R3731	T6088	T6075	punct	.,is
R3732	T6090	T6091	nsubj	AnoG,represents
R3733	T6092	T6093	det	a,protein
R3734	T6093	T6091	dobj	protein,represents
R3735	T6094	T6093	prep	from,protein
R3736	T6095	T6096	det	the,str.
R3737	T6096	T6094	pobj	str.,from
R3738	T6097	T6096	compound	anopheles,str.
R3739	T6098	T6096	compound	gambiae,str.
R374	T686	T675	cc	and,cloned
R3740	T6099	T6100	punct	(,EAA01004
R3741	T6100	T6094	parataxis	EAA01004,from
R3742	T6101	T6100	punct	),EAA01004
R3743	T6102	T6091	punct	.,represents
R3744	T6104	T6105	nsubj	CaeE,is
R3745	T6106	T6104	punct	(,CaeE
R3746	T6107	T6104	appos	AAK77203,CaeE
R3747	T6108	T6105	punct	),is
R3748	T6109	T6110	det	a,protein
R3749	T6110	T6105	attr	protein,is
R375	T687	T675	conj	characterized,cloned
R3750	T6111	T6110	amod	hypothetical,protein
R3751	T6112	T6110	prep	from,protein
R3752	T6113	T6114	det	the,elegans
R3753	T6114	T6112	pobj	elegans,from
R3754	T6115	T6114	compound	Caenohabditis,elegans
R3755	T6116	T6105	punct	.,is
R3756	T6118	T6119	nsubj	CorC,represents
R3757	T6120	T6121	compound	bacteria,magnesium
R3758	T6121	T6119	dobj	magnesium,represents
R3759	T6122	T6121	cc	and,magnesium
R376	T688	T687	dobj	Acdp,characterized
R3760	T6123	T6124	compound	cobalt,protein
R3761	T6124	T6121	conj	protein,magnesium
R3762	T6125	T6124	compound	efflux,protein
R3763	T6126	T6121	prep	from,magnesium
R3764	T6127	T6128	det	the,oneidensis
R3765	T6128	T6126	pobj	oneidensis,from
R3766	T6129	T6128	compound	Shewanella,oneidensis
R3767	T6130	T6119	punct	.,represents
R3768	T6132	T6133	nsubj	XyFD,is
R3769	T6134	T6135	det	a,protein
R377	T689	T688	punct	", ",Acdp
R3770	T6135	T6133	attr	protein,is
R3771	T6136	T6135	amod	hypothetical,protein
R3772	T6137	T6135	prep	from,protein
R3773	T6138	T6139	det	the,Dixon
R3774	T6139	T6137	pobj	Dixon,from
R3775	T6140	T6139	compound	Xylella,Dixon
R3776	T6141	T6139	compound	fastidiosa,Dixon
R3777	T6142	T6135	punct	(,protein
R3778	T6143	T6135	appos	ZP_00038107,protein
R3779	T6144	T6133	punct	),is
R378	T690	T691	det	the,homologue
R3780	T6145	T6133	punct	.,is
R3781	T6153	T6154	amod	Phylogenetic,tree
R3782	T6155	T6154	acl	showing,tree
R3783	T6156	T6155	dobj	relationships,showing
R3784	T6157	T6156	prep	among,relationships
R3785	T6158	T6157	pobj	proteins,among
R3786	T6159	T6158	acl	containing,proteins
R3787	T6160	T6161	det	the,domain
R3788	T6161	T6159	dobj	domain,containing
R3789	T6162	T6161	compound	ACD,domain
R379	T691	T688	appos	homologue,Acdp
R3790	T6163	T6161	prep	from,domain
R3791	T6164	T6165	nmod	figure,2
R3792	T6165	T6163	pobj	2,from
R3793	T6166	T6165	cc	and,2
R3794	T6167	T6165	conj	3,2
R3795	T6168	T6154	punct	.,tree
R3796	T6170	T6171	det	The,tree
R3797	T6171	T6173	nsubjpass	tree,constructed
R3798	T6172	T6171	amod	phylogenetic,tree
R3799	T6174	T6173	auxpass	was,constructed
R38	T261	T259	pobj	family,of
R380	T692	T691	compound	mouse,homologue
R3800	T6175	T6173	prep	according,constructed
R3801	T6176	T6175	prep	to,according
R3802	T6177	T6178	det	the,calculation
R3803	T6178	T6176	pobj	calculation,to
R3804	T6179	T6178	prep	of,calculation
R3805	T6180	T6181	det	the,match
R3806	T6181	T6179	pobj	match,of
R3807	T6182	T6181	amod	best,match
R3808	T6183	T6181	prep	for,match
R3809	T6184	T6185	det	the,sequences
R381	T693	T691	prep	of,homologue
R3810	T6185	T6183	pobj	sequences,for
R3811	T6186	T6185	amod	selected,sequences
R3812	T6187	T6173	punct	.,constructed
R3813	T6189	T6190	nsubj	Abbreviations,are
R3814	T6191	T6189	prep	for,Abbreviations
R3815	T6192	T6193	det	each,protein
R3816	T6193	T6191	pobj	protein,for
R3817	T6194	T6195	det	the,same
R3818	T6195	T6190	attr	same,are
R3819	T6196	T6197	mark	as,presented
R382	T694	T695	det	the,family
R3820	T6197	T6195	advcl	presented,same
R3821	T6198	T6197	prep	in,presented
R3822	T6199	T6198	pobj	figure,in
R3823	T6200	T6199	nummod	3,figure
R3824	T6201	T6190	punct	.,are
R3825	T6228	T6229	nummod	Four,domains
R3826	T6230	T6229	compound	transmembrane,domains
R3827	T6231	T6229	prep	within,domains
R3828	T6232	T6233	compound	Acdp4,protein
R3829	T6233	T6231	pobj	protein,within
R383	T695	T693	pobj	family,of
R3830	T6234	T6229	punct	.,domains
R3831	T6236	T6237	compound	Transmembrane,domains
R3832	T6237	T6238	nsubjpass	domains,predicted
R3833	T6239	T6238	auxpass	were,predicted
R3834	T6240	T6238	prep	by,predicted
R3835	T6241	T6242	det	the,program
R3836	T6242	T6240	pobj	program,by
R3837	T6243	T6242	compound	TMHMM,program
R3838	T6244	T6238	punct	.,predicted
R3839	T6246	T6247	det	The,plot
R384	T696	T695	amod	human,family
R3840	T6247	T6248	nsubj	plot,shows
R3841	T6249	T6250	det	the,probabilities
R3842	T6250	T6248	dobj	probabilities,shows
R3843	T6251	T6250	amod	posterior,probabilities
R3844	T6252	T6250	prep	of,probabilities
R3845	T6253	T6254	amod	inside,TM
R3846	T6254	T6258	compound	TM,helix
R3847	T6255	T6254	punct	/,TM
R3848	T6256	T6254	amod	outside,TM
R3849	T6257	T6254	punct	/,TM
R385	T697	T695	compound	ACDP,family
R3850	T6258	T6252	pobj	helix,of
R3851	T6259	T6248	punct	.,shows
R3852	T6261	T6262	prep	At,shown
R3853	T6263	T6264	det	the,top
R3854	T6264	T6261	pobj	top,At
R3855	T6265	T6264	prep	of,top
R3856	T6266	T6267	det	the,plot
R3857	T6267	T6265	pobj	plot,of
R3858	T6268	T6261	punct	(,At
R3859	T6269	T6261	prep	between,At
R386	T698	T695	compound	gene,family
R3860	T6270	T6269	pobj	1,between
R3861	T6271	T6270	cc	and,1
R3862	T6272	T6270	conj	1.2,1
R3863	T6273	T6262	punct	),shown
R3864	T6274	T6275	det	the,prediction
R3865	T6275	T6262	nsubjpass	prediction,shown
R3866	T6276	T6277	npadvmod	N,best
R3867	T6277	T6275	amod	best,prediction
R3868	T6278	T6277	punct	-,best
R3869	T6279	T6262	auxpass	is,shown
R387	T699	T675	punct	.,cloned
R3870	T6280	T6262	punct	.,shown
R3871	T6282	T6283	det	The,plot
R3872	T6283	T6284	nsubjpass	plot,obtained
R3873	T6285	T6284	auxpass	is,obtained
R3874	T6286	T6284	prep	by,obtained
R3875	T6287	T6286	pcomp	calculating,by
R3876	T6288	T6289	det	the,probability
R3877	T6289	T6287	dobj	probability,calculating
R3878	T6290	T6289	amod	total,probability
R3879	T6291	T6292	mark	that,sits
R388	T863	T864	amod	Molecular,cloning
R3880	T6292	T6289	acl	sits,probability
R3881	T6293	T6294	det	a,residue
R3882	T6294	T6292	nsubj	residue,sits
R3883	T6295	T6292	prep	in,sits
R3884	T6296	T6295	pobj	helix,in
R3885	T6297	T6292	punct	", ",sits
R3886	T6298	T6292	advmod	inside,sits
R3887	T6299	T6298	punct	", ",inside
R3888	T6300	T6298	cc	or,inside
R3889	T6301	T6298	conj	outside,inside
R389	T865	T864	prep	of,cloning
R3890	T6302	T6289	acl	summed,probability
R3891	T6303	T6302	prep	over,summed
R3892	T6304	T6305	det	all,paths
R3893	T6305	T6303	pobj	paths,over
R3894	T6306	T6305	amod	possible,paths
R3895	T6307	T6305	prep	through,paths
R3896	T6308	T6309	det	the,model
R3897	T6309	T6307	pobj	model,through
R3898	T6310	T6284	punct	.,obtained
R3899	T6368	T6369	dep	Fig.,analysis
R39	T262	T261	amod	novel,family
R390	T866	T867	det	the,family
R3900	T6370	T6368	nummod	6A,Fig.
R3901	T6371	T6369	punct	: ,analysis
R3902	T6372	T6369	compound	Immunoblot,analysis
R3903	T6373	T6369	prep	of,analysis
R3904	T6374	T6375	compound	Acdp,proteins
R3905	T6375	T6373	pobj	proteins,of
R3906	T6376	T6375	prep	in,proteins
R3907	T6377	T6378	compound	brain,tissue
R3908	T6378	T6379	compound	tissue,extracts
R3909	T6379	T6376	pobj	extracts,in
R391	T867	T865	pobj	family,of
R3910	T6380	T6369	punct	.,analysis
R3911	T6382	T6383	nsubjpass	Immunoblotting,carried
R3912	T6384	T6383	auxpass	were,carried
R3913	T6385	T6383	prt	out,carried
R3914	T6386	T6383	advcl	using,carried
R3915	T6387	T6388	det	a,system
R3916	T6388	T6386	dobj	system,using
R3917	T6389	T6390	compound	Western,blotting
R3918	T6390	T6388	compound	blotting,system
R3919	T6391	T6388	compound	detection,system
R392	T868	T867	compound	Acdp,family
R3920	T6392	T6393	punct	(,ECL
R3921	T6393	T6386	parataxis	ECL,using
R3922	T6394	T6393	punct	),ECL
R3923	T6395	T6396	punct	(,PIERCE
R3924	T6396	T6386	parataxis	PIERCE,using
R3925	T6397	T6396	punct	),PIERCE
R3926	T6398	T6383	punct	.,carried
R3927	T6400	T6401	nsubj	Lane,probed
R3928	T6402	T6400	nummod	1,Lane
R3929	T6403	T6401	punct	", ",probed
R393	T869	T867	compound	gene,family
R3930	T6404	T6401	prep	with,probed
R3931	T6405	T6404	pobj	antibody,with
R3932	T6406	T6405	acl	generated,antibody
R3933	T6407	T6406	agent	by,generated
R3934	T6408	T6409	amod	conserved,peptide
R3935	T6409	T6407	pobj	peptide,by
R3936	T6410	T6401	punct	.,probed
R3937	T6412	T6413	prep	From,band
R3938	T6414	T6412	pobj	top,From
R3939	T6415	T6412	prep	to,From
R394	T871	T872	aux	To,clone
R3940	T6416	T6415	pobj	bottom,to
R3941	T6417	T6413	punct	", ",band
R3942	T6418	T6413	det	each,band
R3943	T6419	T6413	acl	corresponding,band
R3944	T6420	T6419	prep	to,corresponding
R3945	T6421	T6420	pobj	Acdp1,to
R3946	T6422	T6423	punct	(,kD
R3947	T6423	T6421	parataxis	kD,Acdp1
R3948	T6424	T6423	nummod	115,kD
R3949	T6425	T6423	punct	),kD
R395	T872	T873	advcl	clone,used
R3950	T6426	T6421	punct	", ",Acdp1
R3951	T6427	T6421	conj	Acdp2,Acdp1
R3952	T6428	T6429	punct	(,kD
R3953	T6429	T6427	parataxis	kD,Acdp2
R3954	T6430	T6429	nummod	100,kD
R3955	T6431	T6429	punct	),kD
R3956	T6432	T6427	punct	", ",Acdp2
R3957	T6433	T6427	conj	Acdp4,Acdp2
R3958	T6434	T6435	punct	(,kD
R3959	T6435	T6433	parataxis	kD,Acdp4
R396	T874	T875	det	the,genes
R3960	T6436	T6435	nummod	90,kD
R3961	T6437	T6435	punct	),kD
R3962	T6438	T6433	cc	and,Acdp4
R3963	T6439	T6433	conj	Acdp3,Acdp4
R3964	T6440	T6441	punct	(,kD
R3965	T6441	T6439	parataxis	kD,Acdp3
R3966	T6442	T6441	nummod	80,kD
R3967	T6443	T6441	punct	),kD
R3968	T6444	T6413	punct	.,band
R3969	T6446	T6447	nmod	Lane,2
R397	T875	T872	dobj	genes,clone
R3970	T6447	T6448	nsubj	2,probed
R3971	T6449	T6447	cc	and,2
R3972	T6450	T6447	conj	3,2
R3973	T6451	T6448	punct	", ",probed
R3974	T6452	T6448	prep	with,probed
R3975	T6453	T6454	det	the,antibodies
R3976	T6454	T6452	pobj	antibodies,with
R3977	T6455	T6454	compound	Acdp1,antibodies
R3978	T6456	T6454	acl	generated,antibodies
R3979	T6457	T6456	agent	by,generated
R398	T876	T875	compound	mouse,genes
R3980	T6458	T6459	npadvmod	N,terminal
R3981	T6459	T6461	amod	terminal,peptides
R3982	T6460	T6459	punct	-,terminal
R3983	T6461	T6457	pobj	peptides,by
R3984	T6462	T6459	cc	and,terminal
R3985	T6463	T6464	npadvmod	C,terminal
R3986	T6464	T6459	conj	terminal,terminal
R3987	T6465	T6464	punct	-,terminal
R3988	T6466	T6456	punct	", ",generated
R3989	T6467	T6456	advmod	respectively,generated
R399	T877	T875	compound	Acdp,genes
R3990	T6468	T6448	punct	.,probed
R3991	T6470	T6471	dep	Fig.,analysis
R3992	T6472	T6470	nummod	6B,Fig.
R3993	T6473	T6471	punct	: ,analysis
R3994	T6474	T6471	compound	Immunoblot,analysis
R3995	T6475	T6471	prep	of,analysis
R3996	T6476	T6475	pobj	Acdp1,of
R3997	T6477	T6471	prep	in,analysis
R3998	T6478	T6479	nmod	HEK293,cells
R3999	T6479	T6477	pobj	cells,in
R4	T220	T217	conj	characterization,cloning
R40	T263	T261	compound	gene,family
R400	T878	T873	punct	", ",used
R4000	T6480	T6478	punct	", ",HEK293
R4001	T6481	T6478	conj	3T3,HEK293
R4002	T6482	T6481	cc	and,3T3
R4003	T6483	T6481	conj	PC12,3T3
R4004	T6484	T6471	punct	.,analysis
R4005	T6486	T6487	det	The,blots
R4006	T6487	T6488	nsubjpass	blots,probed
R4007	T6489	T6488	auxpass	were,probed
R4008	T6490	T6488	prep	with,probed
R4009	T6491	T6492	det	the,antibody
R401	T879	T880	det	the,cDNA
R4010	T6492	T6490	pobj	antibody,with
R4011	T6493	T6492	prep	against,antibody
R4012	T6494	T6495	det	the,terminus
R4013	T6495	T6493	pobj	terminus,against
R4014	T6496	T6495	compound	C,terminus
R4015	T6497	T6495	punct	-,terminus
R4016	T6498	T6495	prep	of,terminus
R4017	T6499	T6498	pobj	Acdp,of
R4018	T6500	T6499	punct	-,Acdp
R4019	T6501	T6499	nummod	1,Acdp
R402	T880	T873	nsubjpass	cDNA,used
R4020	T6502	T6488	punct	.,probed
R4021	T6504	T6505	dep	Lane,μg
R4022	T6506	T6504	nummod	1,Lane
R4023	T6507	T6505	punct	", ",μg
R4024	T6508	T6505	nummod	10,μg
R4025	T6509	T6505	prep	of,μg
R4026	T6510	T6511	compound	HEK293,lysates
R4027	T6511	T6509	pobj	lysates,of
R4028	T6512	T6511	compound	cell,lysates
R4029	T6513	T6505	punct	.,μg
R403	T881	T880	amod	human,cDNA
R4030	T6515	T6516	nmod	Lane,2
R4031	T6516	T6517	dep	2,μg
R4032	T6518	T6516	cc	and,2
R4033	T6519	T6516	conj	3,2
R4034	T6520	T6517	punct	", ",μg
R4035	T6521	T6517	nummod	100,μg
R4036	T6522	T6517	prep	of,μg
R4037	T6523	T6524	nmod	3T3,lysates
R4038	T6524	T6522	pobj	lysates,of
R4039	T6525	T6523	cc	and,3T3
R404	T882	T880	compound	ACDP,cDNA
R4040	T6526	T6523	conj	PC12,3T3
R4041	T6527	T6524	compound	cell,lysates
R4042	T6528	T6517	punct	.,μg
R4043	T6553	T6554	amod	Subcellular,localization
R4044	T6555	T6554	prep	of,localization
R4045	T6556	T6555	pobj	Acdp1,of
R4046	T6557	T6554	prep	in,localization
R4047	T6558	T6559	compound	hippocampus,neurons
R4048	T6559	T6557	pobj	neurons,in
R4049	T6560	T6554	punct	.,localization
R405	T883	T880	cc	and,cDNA
R4050	T6562	T6563	det	A,series
R4051	T6564	T6563	prep	of,series
R4052	T6565	T6566	amod	confocal,images
R4053	T6566	T6564	pobj	images,of
R4054	T6567	T6563	prep	from,series
R4055	T6568	T6569	det	a,neuron
R4056	T6569	T6567	pobj	neuron,from
R4057	T6570	T6569	amod	cultured,neuron
R4058	T6571	T6569	acl	stained,neuron
R4059	T6572	T6571	prep	with,stained
R406	T884	T885	amod	predicted,sequences
R4060	T6573	T6574	det	an,antibody
R4061	T6574	T6572	pobj	antibody,with
R4062	T6575	T6574	amod	anti-Acdp1,antibody
R4063	T6576	T6563	punct	.,series
R4064	T6578	T6579	det	The,step
R4065	T6579	T6580	nsubj	step,is
R4066	T6581	T6579	prep	of,step
R4067	T6582	T6583	det	each,section
R4068	T6583	T6581	pobj	section,of
R4069	T6584	T6583	compound	imaging,section
R407	T885	T880	conj	sequences,cDNA
R4070	T6585	T6586	nummod	0.5,μm
R4071	T6586	T6580	attr	μm,is
R4072	T6587	T6580	punct	", ",is
R4073	T6588	T6580	prep	from,is
R4074	T6589	T6590	det	the,surface
R4075	T6590	T6588	pobj	surface,from
R4076	T6591	T6590	prep	of,surface
R4077	T6592	T6593	det	the,neuron
R4078	T6593	T6591	pobj	neuron,of
R4079	T6594	T6595	punct	(,μm
R408	T886	T885	compound	protein,sequences
R4080	T6595	T6590	parataxis	μm,surface
R4081	T6596	T6595	nummod	0,μm
R4082	T6597	T6595	punct	),μm
R4083	T6598	T6588	prep	to,from
R4084	T6599	T6600	det	the,plan
R4085	T6600	T6598	pobj	plan,to
R4086	T6601	T6600	amod	middle,plan
R4087	T6602	T6603	punct	(,μm
R4088	T6603	T6600	parataxis	μm,plan
R4089	T6604	T6603	nummod	4.5,μm
R409	T887	T873	auxpass	were,used
R4090	T6605	T6603	punct	),μm
R4091	T6606	T6580	punct	.,is
R4092	T6623	T6624	nmod	Nucleotide,homologies
R4093	T6625	T6623	cc	and,Nucleotide
R4094	T6626	T6627	compound	amino,acid
R4095	T6627	T6623	conj	acid,Nucleotide
R4096	T6628	T6629	punct	(,%
R4097	T6629	T6624	parataxis	%,homologies
R4098	T6630	T6629	punct	),%
R4099	T6631	T6624	prep	between,homologies
R41	T264	T255	punct	", ",cloned
R410	T888	T889	aux	to,search
R4100	T6632	T6633	amod	human,ACDP
R4101	T6633	T6634	nmod	ACDP,members
R4102	T6634	T6631	pobj	members,between
R4103	T6635	T6633	cc	and,ACDP
R4104	T6636	T6637	compound	mouse,Acdp
R4105	T6637	T6633	conj	Acdp,ACDP
R4106	T6638	T6624	punct	.,homologies
R411	T889	T873	advcl	search,used
R412	T890	T891	det	the,database
R413	T891	T889	dobj	database,search
R414	T892	T891	compound	mouse,database
R415	T893	T891	compound	EST,database
R416	T894	T889	prep	with,search
R417	T895	T896	det	the,programs
R418	T896	T894	pobj	programs,with
R419	T897	T896	nmod	blastn,programs
R42	T265	T255	nsubj	we,cloned
R420	T898	T897	cc	and,blastn
R421	T899	T897	conj	tblastn,blastn
R422	T900	T873	punct	.,used
R423	T902	T903	compound	Mouse,markers
R424	T903	T905	nsubjpass	markers,identified
R425	T904	T903	compound	EST,markers
R426	T906	T903	acl	corresponding,markers
R427	T907	T906	prep	to,corresponding
R428	T908	T909	det	each,member
R429	T909	T907	pobj	member,to
R43	T266	T255	aux	have,cloned
R430	T910	T909	compound	Acdp,member
R431	T911	T905	auxpass	were,identified
R432	T912	T905	advmod	then,identified
R433	T913	T905	punct	.,identified
R434	T915	T916	prep	For,corresponds
R435	T917	T915	pobj	example,For
R436	T918	T916	punct	", ",corresponds
R437	T919	T920	compound	EST,H3086H12
R438	T920	T916	nsubj	H3086H12,corresponds
R439	T921	T920	punct	-,H3086H12
R44	T267	T255	cc	and,cloned
R440	T922	T920	nummod	5,H3086H12
R441	T923	T916	prep	to,corresponds
R442	T924	T923	pobj	Acdp1,to
R443	T925	T916	punct	", ",corresponds
R444	T926	T916	conj	W98010,corresponds
R445	T927	T926	prep	for,W98010
R446	T928	T927	pobj	Acdp2,for
R447	T929	T926	punct	", ",W98010
R448	T930	T926	conj	603299135F1,W98010
R449	T931	T930	prep	for,603299135F1
R45	T268	T255	conj	characterized,cloned
R450	T932	T931	pobj	Acdp3,for
R451	T933	T930	cc	and,603299135F1
R452	T934	T930	conj	BG083791,603299135F1
R453	T935	T934	prep	for,BG083791
R454	T936	T935	pobj	Acdp4,for
R455	T937	T916	punct	.,corresponds
R456	T939	T940	det	A,dT
R457	T940	T944	nsubjpass	dT,used
R458	T941	T940	amod	modified,dT
R459	T942	T940	compound	oligo,dT
R46	T269	T268	dobj	Acdp,characterized
R460	T943	T940	punct	-,dT
R461	T945	T940	prep	with,dT
R462	T946	T947	det	a,tail
R463	T947	T945	pobj	tail,with
R464	T948	T947	compound	M13,tail
R465	T949	T944	auxpass	was,used
R466	T950	T944	prep	for,used
R467	T951	T952	det	the,reaction
R468	T952	T950	pobj	reaction,for
R469	T953	T952	compound	RT,reaction
R47	T270	T269	punct	", ",Acdp
R470	T954	T944	punct	.,used
R471	T956	T957	det	A,primer
R472	T957	T959	nsubjpass	primer,used
R473	T958	T957	amod	forward,primer
R474	T960	T957	prep	from,primer
R475	T961	T962	det	each,marker
R476	T962	T960	pobj	marker,from
R477	T963	T962	compound	EST,marker
R478	T964	T962	cc	and,marker
R479	T965	T966	det	the,primer
R48	T271	T272	det	the,homologue
R480	T966	T962	conj	primer,marker
R481	T967	T966	compound	M13,primer
R482	T968	T966	punct	(,primer
R483	T969	T970	compound	olig,dT
R484	T970	T972	compound	dT,tail
R485	T971	T970	punct	-,dT
R486	T972	T966	appos	tail,primer
R487	T973	T966	punct	),primer
R488	T974	T959	auxpass	were,used
R489	T975	T976	aux	to,amplify
R49	T272	T269	appos	homologue,Acdp
R490	T976	T959	advcl	amplify,used
R491	T977	T978	det	the,sequence
R492	T978	T976	dobj	sequence,amplify
R493	T979	T980	nummod	3,UTR
R494	T980	T978	compound	UTR,sequence
R495	T981	T979	punct	',3
R496	T982	T976	prep	for,amplify
R497	T983	T984	det	each,gene
R498	T984	T982	pobj	gene,for
R499	T985	T984	amod	corresponding,gene
R5	T221	T220	prep	of,characterization
R50	T273	T272	compound	mouse,homologue
R500	T986	T984	compound	Acdp,gene
R501	T987	T984	prep	from,gene
R502	T988	T989	det	the,products
R503	T989	T987	pobj	products,from
R504	T990	T989	compound	RT,products
R505	T991	T959	punct	.,used
R506	T993	T994	aux	To,obtain
R507	T994	T995	advcl	obtain,conducted
R508	T996	T997	nummod	5,end
R509	T997	T1000	nmod	end,sequences
R51	T274	T272	prep	of,homologue
R510	T998	T996	punct	',5
R511	T999	T997	punct	-,end
R512	T1000	T994	dobj	sequences,obtain
R513	T1001	T1000	amod	coding,sequences
R514	T1002	T994	prep	for,obtain
R515	T1003	T1004	det	the,genes
R516	T1004	T1002	pobj	genes,for
R517	T1005	T1004	compound	Acdp,genes
R518	T1006	T995	punct	", ",conducted
R519	T1007	T995	nsubj	we,conducted
R52	T275	T276	det	the,family
R520	T1008	T1009	det	a,PCR
R521	T1009	T995	dobj	PCR,conducted
R522	T1010	T1011	npadvmod	series,nested
R523	T1011	T1009	amod	nested,PCR
R524	T1012	T995	prep	with,conducted
R525	T1013	T1012	pobj	combinations,with
R526	T1014	T1013	prep	of,combinations
R527	T1015	T1016	nmod	mouse,primers
R528	T1016	T1014	pobj	primers,of
R529	T1017	T1015	cc	and,mouse
R53	T276	T274	pobj	family,of
R530	T1018	T1015	conj	human,mouse
R531	T1019	T995	punct	.,conducted
R532	T1021	T1022	det	The,sequences
R533	T1022	T1026	nsubjpass	sequences,identified
R534	T1023	T1022	nummod	5,sequences
R535	T1024	T1023	punct	',5
R536	T1025	T1022	compound	UTR,sequences
R537	T1027	T1026	auxpass	were,identified
R538	T1028	T1026	prep	by,identified
R539	T1029	T1030	advmod	directly,sequencing
R54	T277	T276	amod	human,family
R540	T1030	T1028	pcomp	sequencing,by
R541	T1031	T1032	compound	BAC,DNA
R542	T1032	T1030	dobj	DNA,sequencing
R543	T1033	T1032	acl	containing,DNA
R544	T1034	T1035	det	the,genes
R545	T1035	T1033	dobj	genes,containing
R546	T1036	T1035	amod	corresponding,genes
R547	T1037	T1035	compound	Acdp,genes
R548	T1038	T1026	punct	.,identified
R549	T1040	T1041	det	The,clones
R55	T278	T276	compound	ACDP,family
R550	T1041	T1043	nsubjpass	clones,identified
R551	T1042	T1041	compound	BAC,clones
R552	T1044	T1043	auxpass	were,identified
R553	T1045	T1043	prep	by,identified
R554	T1046	T1045	pcomp	screening,by
R555	T1047	T1048	det	a,library
R556	T1048	T1046	dobj	library,screening
R557	T1049	T1050	compound	CITB,DNA
R558	T1050	T1048	compound	DNA,library
R559	T1051	T1050	compound	mouse,DNA
R56	T279	T276	compound	gene,family
R560	T1052	T1050	compound	BAC,DNA
R561	T1053	T1054	punct	(,Genetics
R562	T1054	T1048	parataxis	Genetics,library
R563	T1055	T1054	compound	Research,Genetics
R564	T1056	T1054	punct	),Genetics
R565	T1057	T1043	punct	.,identified
R566	T1059	T1060	det	The,sequences
R567	T1060	T1064	nsubjpass	sequences,confirmed
R568	T1061	T1060	nummod	5,sequences
R569	T1062	T1061	punct	',5
R57	T280	T255	punct	.,cloned
R570	T1063	T1060	compound	UTR,sequences
R571	T1065	T1060	acl	obtained,sequences
R572	T1066	T1065	prep	from,obtained
R573	T1067	T1066	pcomp	above,from
R574	T1068	T1064	auxpass	were,confirmed
R575	T1069	T1064	advmod	further,confirmed
R576	T1070	T1064	prep	by,confirmed
R577	T1071	T1072	compound	RT,PCR
R578	T1072	T1070	pobj	PCR,by
R579	T1073	T1072	punct	-,PCR
R58	T284	T285	det	The,genes
R580	T1074	T1064	punct	.,confirmed
R581	T1076	T1077	det	The,gene
R582	T1077	T1079	nsubj	gene,contains
R583	T1078	T1077	compound	Acdp1,gene
R584	T1080	T1081	nummod	"3,631",bp
R585	T1081	T1079	dobj	bp,contains
R586	T1082	T1081	prep	of,bp
R587	T1083	T1084	compound	nucleotide,sequence
R588	T1084	T1082	pobj	sequence,of
R589	T1085	T1079	cc	and,contains
R59	T285	T288	nsubj	genes,contain
R590	T1086	T1079	conj	encodes,contains
R591	T1087	T1088	det	a,protein
R592	T1088	T1086	dobj	protein,encodes
R593	T1089	T1088	amod	predicted,protein
R594	T1090	T1086	prep	with,encodes
R595	T1091	T1092	nummod	951,acids
R596	T1092	T1090	pobj	acids,with
R597	T1093	T1092	compound	amino,acids
R598	T1094	T1095	punct	(,AA
R599	T1095	T1086	parataxis	AA,encodes
R6	T222	T223	det	the,family
R60	T286	T285	nummod	four,genes
R600	T1096	T1095	punct	),AA
R601	T1097	T1079	punct	.,contains
R602	T1099	T1100	det	The,genes
R603	T1100	T1104	nsubj	genes,contain
R604	T1101	T1100	amod	other,genes
R605	T1102	T1100	nummod	three,genes
R606	T1103	T1100	compound	Acdp,genes
R607	T1105	T1100	punct	(,genes
R608	T1106	T1100	appos	Acdp2,genes
R609	T1107	T1106	punct	", ",Acdp2
R61	T287	T285	compound	Acdp,genes
R610	T1108	T1106	nummod	3,Acdp2
R611	T1109	T1106	cc	and,Acdp2
R612	T1110	T1106	conj	4,Acdp2
R613	T1111	T1100	punct	),genes
R614	T1112	T1113	nummod	"3,244",bp
R615	T1113	T1104	dobj	bp,contain
R616	T1114	T1113	punct	", ",bp
R617	T1115	T1116	nummod	"2,684",bp
R618	T1116	T1113	conj	bp,bp
R619	T1117	T1116	cc	and,bp
R62	T289	T285	punct	(,genes
R620	T1118	T1119	nummod	"2,743",bp
R621	T1119	T1116	conj	bp,bp
R622	T1120	T1113	prep	of,bp
R623	T1121	T1122	compound	cDNA,sequences
R624	T1122	T1120	pobj	sequences,of
R625	T1123	T1104	punct	", ",contain
R626	T1124	T1104	cc	and,contain
R627	T1125	T1104	conj	encode,contain
R628	T1126	T1127	amod	deduced,proteins
R629	T1127	T1125	dobj	proteins,encode
R63	T290	T285	appos	Acdp1,genes
R630	T1128	T1127	prep	of,proteins
R631	T1129	T1130	nummod	874,acids
R632	T1130	T1128	pobj	acids,of
R633	T1131	T1130	compound	amino,acids
R634	T1132	T1130	punct	", ",acids
R635	T1133	T1134	nummod	713,acids
R636	T1134	T1130	conj	acids,acids
R637	T1135	T1134	compound	amino,acids
R638	T1136	T1134	cc	and,acids
R639	T1137	T1138	nummod	771,acids
R64	T291	T290	punct	", ",Acdp1
R640	T1138	T1134	conj	acids,acids
R641	T1139	T1138	compound	amino,acids
R642	T1140	T1125	punct	", ",encode
R643	T1141	T1125	advmod	respectively,encode
R644	T1142	T1104	punct	.,contain
R645	T1296	T1297	compound	Tissue,distribution
R646	T1299	T1300	compound	Northern,blot
R647	T1300	T1301	compound	blot,analyses
R648	T1301	T1302	nsubjpass	analyses,carried
R649	T1303	T1301	prep	of,analyses
R65	T292	T290	conj	Acdp2,Acdp1
R650	T1304	T1305	det	the,family
R651	T1305	T1303	pobj	family,of
R652	T1306	T1305	compound	Acdp,family
R653	T1307	T1305	compound	gene,family
R654	T1308	T1302	auxpass	were,carried
R655	T1309	T1302	prt	out,carried
R656	T1310	T1302	advcl	using,carried
R657	T1311	T1310	dobj	membranes,using
R658	T1312	T1311	acl	purchased,membranes
R659	T1313	T1312	prep	from,purchased
R66	T293	T292	punct	", ",Acdp2
R660	T1314	T1313	pobj	Origene,from
R661	T1315	T1302	punct	.,carried
R662	T1317	T1318	det	A,total
R663	T1318	T1319	nsubjpass	total,included
R664	T1320	T1318	prep	of,total
R665	T1321	T1322	nummod	12,tissues
R666	T1322	T1320	pobj	tissues,of
R667	T1323	T1322	compound	mouse,tissues
R668	T1324	T1319	auxpass	were,included
R669	T1325	T1319	prep	in,included
R67	T294	T292	conj	Acdp3,Acdp2
R670	T1326	T1327	det	the,study
R671	T1327	T1325	pobj	study,in
R672	T1328	T1329	punct	(,Fig.
R673	T1329	T1319	parataxis	Fig.,included
R674	T1330	T1329	nummod	1,Fig.
R675	T1331	T1329	punct	),Fig.
R676	T1332	T1319	punct	.,included
R677	T1334	T1335	prep	Due,were
R678	T1336	T1334	pcomp	to,Due
R679	T1337	T1338	compound	sequence,homologies
R68	T295	T294	cc	and,Acdp3
R680	T1338	T1334	pobj	homologies,Due
R681	T1339	T1338	prep	between,homologies
R682	T1340	T1341	det	each,member
R683	T1341	T1339	pobj	member,between
R684	T1342	T1341	compound	Acdp,member
R685	T1343	T1341	prep	within,member
R686	T1344	T1345	det	the,domain
R687	T1345	T1343	pobj	domain,within
R688	T1346	T1345	amod	conserved,domain
R689	T1347	T1335	punct	", ",were
R69	T296	T294	conj	Acdp4,Acdp3
R690	T1348	T1335	nsubj	probes,were
R691	T1349	T1348	prep	for,probes
R692	T1350	T1351	compound	Northern,bolts
R693	T1351	T1349	pobj	bolts,for
R694	T1352	T1353	compound	PCR,fragments
R695	T1353	T1335	attr	fragments,were
R696	T1354	T1353	prep	from,fragments
R697	T1355	T1356	det	the,exon
R698	T1356	T1354	pobj	exon,from
R699	T1357	T1356	amod	last,exon
R7	T223	T221	pobj	family,of
R70	T297	T288	punct	),contain
R700	T1358	T1356	cc	and,exon
R701	T1359	T1360	det	the,sequences
R702	T1360	T1356	conj	sequences,exon
R703	T1361	T1362	nummod	3,region
R704	T1362	T1360	compound	region,sequences
R705	T1363	T1361	punct	',3
R706	T1364	T1362	amod	untranslated,region
R707	T1365	T1335	punct	.,were
R708	T1367	T1368	det	The,messages
R709	T1368	T1371	nsubj	messages,showed
R71	T298	T299	nummod	"3,631",bp
R710	T1369	T1368	compound	mouse,messages
R711	T1370	T1368	compound	Acdp,messages
R712	T1372	T1373	advmod	almost,distributions
R713	T1373	T1371	dobj	distributions,showed
R714	T1374	T1373	det	the,distributions
R715	T1375	T1373	amod	same,distributions
R716	T1376	T1373	compound	tissue,distributions
R717	T1377	T1373	prep	as,distributions
R718	T1378	T1379	det	the,genes
R719	T1379	T1377	pobj	genes,as
R72	T299	T288	dobj	bp,contain
R720	T1380	T1379	amod	human,genes
R721	T1381	T1379	compound	ACDP,genes
R722	T1382	T1371	punct	.,showed
R723	T1384	T1385	compound	Acdp1,message
R724	T1385	T1386	nsubjpass	message,expressed
R725	T1387	T1386	auxpass	is,expressed
R726	T1388	T1386	advmod	highly,expressed
R727	T1389	T1386	prep	in,expressed
R728	T1390	T1391	det	the,brain
R729	T1391	T1389	pobj	brain,in
R73	T300	T299	punct	", ",bp
R730	T1392	T1386	punct	", ",expressed
R731	T1393	T1394	mark	while,showed
R732	T1394	T1386	advcl	showed,expressed
R733	T1395	T1394	nsubj	kidney,showed
R734	T1396	T1395	cc	and,kidney
R735	T1397	T1395	conj	testis,kidney
R736	T1398	T1394	advmod	also,showed
R737	T1399	T1400	amod	low,levels
R738	T1400	T1394	dobj	levels,showed
R739	T1401	T1400	prep	of,levels
R74	T301	T302	nummod	"3,244",bp
R740	T1402	T1401	pobj	expression,of
R741	T1403	T1386	punct	.,expressed
R742	T1405	T1406	nsubj	Acdp2,showed
R743	T1407	T1408	amod	higher,expressions
R744	T1408	T1406	dobj	expressions,showed
R745	T1409	T1406	prep	in,showed
R746	T1410	T1411	det	the,brain
R747	T1411	T1409	pobj	brain,in
R748	T1412	T1411	punct	", ",brain
R749	T1413	T1411	conj	kidney,brain
R75	T302	T299	conj	bp,bp
R750	T1414	T1413	cc	and,kidney
R751	T1415	T1413	conj	liver,kidney
R752	T1416	T1406	punct	.,showed
R753	T1418	T1419	advmod	However,was
R754	T1420	T1419	punct	", ",was
R755	T1421	T1422	det	the,transcript
R756	T1422	T1419	nsubj	transcript,was
R757	T1423	T1422	compound	Acdp2,transcript
R758	T1424	T1419	neg	not,was
R759	T1425	T1419	acomp	present,was
R76	T303	T302	punct	", ",bp
R760	T1426	T1419	prep	in,was
R761	T1427	T1428	det	the,muscle
R762	T1428	T1426	pobj	muscle,in
R763	T1429	T1428	compound	skeleton,muscle
R764	T1430	T1428	cc	and,muscle
R765	T1431	T1428	conj	skin,muscle
R766	T1432	T1419	punct	", ",was
R767	T1433	T1419	cc	and,was
R768	T1434	T1435	nsubj	it,showed
R769	T1435	T1419	conj	showed,was
R77	T304	T305	nummod	"2,684",bp
R770	T1436	T1437	advmod	very,low
R771	T1437	T1438	amod	low,levels
R772	T1438	T1435	dobj	levels,showed
R773	T1439	T1438	prep	of,levels
R774	T1440	T1439	pobj	expression,of
R775	T1441	T1435	prep	in,showed
R776	T1442	T1443	det	the,rest
R777	T1443	T1441	pobj	rest,in
R778	T1444	T1443	prep	of,rest
R779	T1445	T1444	pobj	tissues,of
R78	T305	T302	conj	bp,bp
R780	T1446	T1435	punct	.,showed
R781	T1448	T1449	nsubj	Acdp3,showed
R782	T1449	T1452	ccomp	showed,observed
R783	T1450	T1448	cc	and,Acdp3
R784	T1451	T1448	conj	Acdp4,Acdp3
R785	T1453	T1454	amod	different,levels
R786	T1454	T1449	dobj	levels,showed
R787	T1455	T1454	prep	of,levels
R788	T1456	T1455	pobj	expression,of
R789	T1457	T1449	prep	in,showed
R79	T306	T305	cc	and,bp
R790	T1458	T1459	det	all,tissues
R791	T1459	T1457	pobj	tissues,in
R792	T1460	T1459	acl	tested,tissues
R793	T1461	T1452	punct	;,observed
R794	T1462	T1463	det	the,expressions
R795	T1463	T1452	nsubjpass	expressions,observed
R796	T1464	T1463	amod	highest,expressions
R797	T1465	T1463	prep	for,expressions
R798	T1466	T1465	pobj	Acdp3,for
R799	T1467	T1452	auxpass	were,observed
R8	T224	T223	compound	mouse,family
R80	T307	T308	nummod	"2,743",bp
R800	T1468	T1452	prep	in,observed
R801	T1469	T1470	det	the,brain
R802	T1470	T1468	pobj	brain,in
R803	T1471	T1470	punct	", ",brain
R804	T1472	T1470	conj	kidney,brain
R805	T1473	T1472	punct	", ",kidney
R806	T1474	T1472	conj	liver,kidney
R807	T1475	T1474	cc	and,liver
R808	T1476	T1474	conj	heart,liver
R809	T1477	T1452	punct	", ",observed
R81	T308	T305	conj	bp,bp
R810	T1478	T1452	cc	and,observed
R811	T1479	T1480	det	the,expressions
R812	T1480	T1482	nsubjpass	expressions,observed
R813	T1481	T1480	amod	highest,expressions
R814	T1482	T1452	conj	observed,observed
R815	T1483	T1480	prep	for,expressions
R816	T1484	T1483	pobj	Acdp4,for
R817	T1485	T1482	auxpass	were,observed
R818	T1486	T1482	prep	in,observed
R819	T1487	T1488	det	the,kidney
R82	T309	T299	prep	of,bp
R820	T1488	T1486	pobj	kidney,in
R821	T1489	T1488	punct	", ",kidney
R822	T1490	T1491	amod	small,intestine
R823	T1491	T1488	conj	intestine,kidney
R824	T1492	T1491	cc	and,intestine
R825	T1493	T1491	conj	testis,intestine
R826	T1494	T1452	punct	.,observed
R827	T1496	T1497	det	The,levels
R828	T1497	T1499	nsubj	levels,were
R829	T1498	T1497	compound	expression,levels
R83	T310	T311	compound	cDNA,sequences
R830	T1499	T1507	ccomp	were,showed
R831	T1500	T1497	prep	for,levels
R832	T1501	T1500	pobj	Acdp3,for
R833	T1502	T1501	cc	and,Acdp3
R834	T1503	T1501	conj	4,Acdp3
R835	T1504	T1497	prep	in,levels
R836	T1505	T1506	compound	skeleton,muscle
R837	T1506	T1504	pobj	muscle,in
R838	T1508	T1509	advmod	barely,detectable
R839	T1509	T1499	acomp	detectable,were
R84	T311	T309	pobj	sequences,of
R840	T1510	T1507	punct	;,showed
R841	T1511	T1507	advmod	however,showed
R842	T1512	T1507	punct	", ",showed
R843	T1513	T1514	compound	β,actin
R844	T1514	T1507	nsubj	actin,showed
R845	T1515	T1514	punct	-,actin
R846	T1516	T1517	amod	normal,expression
R847	T1517	T1507	dobj	expression,showed
R848	T1518	T1507	advcl	suggesting,showed
R849	T1519	T1520	mark	that,were
R85	T312	T288	punct	", ",contain
R850	T1520	T1518	ccomp	were,suggesting
R851	T1521	T1522	det	the,results
R852	T1522	T1520	nsubj	results,were
R853	T1523	T1520	neg	not,were
R854	T1524	T1525	det	a,consequence
R855	T1525	T1520	attr	consequence,were
R856	T1526	T1525	prep	of,consequence
R857	T1527	T1528	amod	bad,quality
R858	T1528	T1526	pobj	quality,of
R859	T1529	T1528	compound	RNA,quality
R86	T313	T288	cc	and,contain
R860	T1530	T1531	punct	(,shown
R861	T1531	T1507	parataxis	shown,showed
R862	T1532	T1531	nsubj	data,shown
R863	T1533	T1531	neg	not,shown
R864	T1534	T1531	punct	),shown
R865	T1535	T1507	punct	.,showed
R866	T1537	T1538	det	The,pattern
R867	T1538	T1541	nsubjpass	pattern,taken
R868	T1539	T1538	amod	ubiquitous,pattern
R869	T1540	T1538	compound	expression,pattern
R87	T314	T288	conj	encode,contain
R870	T1542	T1541	aux	may,taken
R871	T1543	T1541	auxpass	be,taken
R872	T1544	T1541	prep	as,taken
R873	T1545	T1546	det	another,indication
R874	T1546	T1544	pobj	indication,as
R875	T1547	T1546	prep	of,indication
R876	T1548	T1549	det	the,importance
R877	T1549	T1547	pobj	importance,of
R878	T1550	T1549	amod	functional,importance
R879	T1551	T1549	prep	of,importance
R88	T315	T316	amod	deduced,proteins
R880	T1552	T1553	compound	Acdp,proteins
R881	T1553	T1551	pobj	proteins,of
R882	T1554	T1549	prep	in,importance
R883	T1555	T1556	amod	fundamental,processes
R884	T1556	T1554	pobj	processes,in
R885	T1557	T1556	amod	biological,processes
R886	T1558	T1541	prep	in,taken
R887	T1559	T1558	pobj	addition,in
R888	T1560	T1559	prep	to,addition
R889	T1561	T1562	det	the,conservation
R89	T316	T314	dobj	proteins,encode
R890	T1562	T1560	pobj	conservation,to
R891	T1563	T1562	compound	sequence,conservation
R892	T1564	T1562	prep	in,conservation
R893	T1565	T1566	advmod	evolutionarily,divergent
R894	T1566	T1567	amod	divergent,species
R895	T1567	T1564	pobj	species,in
R896	T1568	T1541	punct	.,taken
R897	T1609	T1610	amod	Chromosomal,location
R898	T1612	T1613	compound	Radiation,mapping
R899	T1613	T1615	nsubj	mapping,indicated
R9	T225	T223	compound	Acdp,family
R90	T317	T316	prep	of,proteins
R900	T1614	T1613	compound	hybrid,mapping
R901	T1616	T1617	mark	that,maps
R902	T1617	T1615	ccomp	maps,indicated
R903	T1618	T1619	det	the,gene
R904	T1619	T1617	nsubj	gene,maps
R905	T1620	T1619	compound	Acdp1,gene
R906	T1621	T1617	prep	to,maps
R907	T1622	T1621	pobj	chromosome,to
R908	T1623	T1622	nummod	19,chromosome
R909	T1624	T1617	prep	between,maps
R91	T318	T319	nummod	951,acids
R910	T1625	T1626	compound	markers,D19Mit119
R911	T1626	T1624	pobj	D19Mit119,between
R912	T1627	T1628	punct	(,cR
R913	T1628	T1626	parataxis	cR,D19Mit119
R914	T1629	T1628	nummod	34.3,cR
R915	T1630	T1628	amod	proximal,cR
R916	T1631	T1628	punct	),cR
R917	T1632	T1626	cc	and,D19Mit119
R918	T1633	T1626	conj	D19Mit112,D19Mit119
R919	T1634	T1635	punct	(,cR
R92	T319	T317	pobj	acids,of
R920	T1635	T1633	parataxis	cR,D19Mit112
R921	T1636	T1635	nummod	13.6,cR
R922	T1637	T1635	amod	distal,cR
R923	T1638	T1635	punct	),cR
R924	T1639	T1615	punct	.,indicated
R925	T1641	T1642	det	The,gene
R926	T1642	T1644	nsubj	gene,maps
R927	T1643	T1642	compound	Acdp2,gene
R928	T1645	T1646	advmod	slightly,more
R929	T1646	T1647	advmod	more,distal
R93	T320	T318	punct	", ",951
R930	T1647	T1644	advcl	distal,maps
R931	T1648	T1644	prep	to,maps
R932	T1649	T1650	det	the,Acdp1
R933	T1650	T1648	pobj	Acdp1,to
R934	T1651	T1650	prep	on,Acdp1
R935	T1652	T1651	pobj	chromosome,on
R936	T1653	T1652	nummod	19,chromosome
R937	T1654	T1644	prep	between,maps
R938	T1655	T1654	pobj	D19Mit9,between
R939	T1656	T1657	punct	(,cR
R94	T321	T318	conj	874,951
R940	T1657	T1655	parataxis	cR,D19Mit9
R941	T1658	T1657	nummod	2.4,cR
R942	T1659	T1657	amod	proximal,cR
R943	T1660	T1657	punct	),cR
R944	T1661	T1655	cc	and,D19Mit9
R945	T1662	T1655	conj	D19Mit38,D19Mit9
R946	T1663	T1664	punct	(,cR
R947	T1664	T1662	parataxis	cR,D19Mit38
R948	T1665	T1664	nummod	15.1,cR
R949	T1666	T1664	amod	distal,cR
R95	T322	T321	punct	", ",874
R950	T1667	T1664	punct	),cR
R951	T1668	T1644	punct	.,maps
R952	T1670	T1671	det	The,genes
R953	T1671	T1675	nsubj	genes,map
R954	T1672	T1671	nmod	Acdp3,genes
R955	T1673	T1672	cc	and,Acdp3
R956	T1674	T1672	conj	Acdp4,Acdp3
R957	T1676	T1675	prep	to,map
R958	T1677	T1676	pobj	chromosome,to
R959	T1678	T1677	nummod	1,chromosome
R96	T323	T321	conj	713,874
R960	T1679	T1675	prep	within,map
R961	T1680	T1681	nummod	one,clone
R962	T1681	T1679	pobj	clone,within
R963	T1682	T1681	compound	BAC,clone
R964	T1683	T1684	punct	(,294I17
R965	T1684	T1681	parataxis	294I17,clone
R966	T1685	T1684	compound	RP23,294I17
R967	T1686	T1684	punct	-,294I17
R968	T1687	T1684	punct	),294I17
R969	T1688	T1681	punct	", ",clone
R97	T324	T323	cc	and,713
R970	T1689	T1681	amod	proximal,clone
R971	T1690	T1689	prep	to,proximal
R972	T1691	T1692	compound	marker,D1Mit171
R973	T1692	T1690	pobj	D1Mit171,to
R974	T1693	T1694	punct	(,cR
R975	T1694	T1692	parataxis	cR,D1Mit171
R976	T1695	T1694	nummod	17.4,cR
R977	T1696	T1694	punct	),cR
R978	T1697	T1675	punct	.,map
R979	T1699	T1700	det	These,regions
R98	T325	T323	conj	771,713
R980	T1700	T1701	nsubj	regions,are
R981	T1702	T1701	acomp	syntenic,are
R982	T1703	T1702	prep	to,syntenic
R983	T1704	T1705	det	the,counterparts
R984	T1705	T1703	pobj	counterparts,to
R985	T1706	T1705	amod	human,counterparts
R986	T1707	T1701	punct	.,are
R987	T2019	T2020	compound	Sequence,homology
R988	T2021	T2020	cc	and,homology
R989	T2022	T2023	amod	molecular,characteristics
R99	T326	T319	compound	amino,acids
R990	T2023	T2020	conj	characteristics,homology
R991	T2025	T2026	det	The,genes
R992	T2026	T2029	nsubj	genes,showed
R993	T2027	T2026	compound	mouse,genes
R994	T2028	T2026	compound	Acdp,genes
R995	T2030	T2031	advmod	very,strong
R996	T2031	T2032	amod	strong,homologies
R997	T2032	T2029	dobj	homologies,showed
R998	T2033	T2032	prep	of,homologies
R999	T2034	T2035	preconj	both,nucleotide

PMC:340383 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

PMC:340383 JSON TXT 9 Projects